LOCUS       DAZFRE010000001       129951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFRE010000001 DAZFRE010000000
VERSION     DAZFRE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 129951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.001"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            134..352
                     /gene="tdcR"
                     /locus_tag="ACHKUI_000001"
     CDS             134..352
                     /gene="tdcR"
                     /locus_tag="ACHKUI_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO6596292.1"
                     /translation="MSKFSNFIINKPFSAINNAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            608..700
                     /locus_tag="ACHKUI_000002"
                     /pseudo
     CDS             608..700
                     /locus_tag="ACHKUI_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            703..765
                     /locus_tag="ACHKUI_000003"
                     /pseudo
     CDS             703..765
                     /locus_tag="ACHKUI_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(909..1285)
                     /gene="rnpB"
                     /locus_tag="ACHKUI_000004"
     ncRNA           complement(909..1285)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKUI_000004"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1318..2463)
                     /gene="garK"
                     /locus_tag="ACHKUI_000005"
     CDS             complement(1318..2463)
                     /gene="garK"
                     /locus_tag="ACHKUI_000005"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO6596293.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2560..3450)
                     /gene="garR"
                     /locus_tag="ACHKUI_000006"
     CDS             complement(2560..3450)
                     /gene="garR"
                     /locus_tag="ACHKUI_000006"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6596294.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGM
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3480..4250)
                     /gene="garL"
                     /locus_tag="ACHKUI_000007"
     CDS             complement(3480..4250)
                     /gene="garL"
                     /locus_tag="ACHKUI_000007"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6596295.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGIFRSATQKLADTFKK"
     gene            complement(4266..5600)
                     /gene="garP"
                     /locus_tag="ACHKUI_000008"
     CDS             complement(4266..5600)
                     /gene="garP"
                     /locus_tag="ACHKUI_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6596296.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5975..7546
                     /gene="garD"
                     /locus_tag="ACHKUI_000009"
     CDS             5975..7546
                     /gene="garD"
                     /locus_tag="ACHKUI_000009"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6596297.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTNDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7695..8030
                     /gene="prlF"
                     /locus_tag="ACHKUI_000010"
     CDS             7695..8030
                     /gene="prlF"
                     /locus_tag="ACHKUI_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6596298.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            8030..8494
                     /gene="yhaV"
                     /locus_tag="ACHKUI_000011"
     CDS             8030..8494
                     /gene="yhaV"
                     /locus_tag="ACHKUI_000011"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6596299.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8549..9358)
                     /gene="agaR"
                     /locus_tag="ACHKUI_000012"
     CDS             complement(8549..9358)
                     /gene="agaR"
                     /locus_tag="ACHKUI_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6596300.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9607..10887
                     /gene="kbaZ"
                     /locus_tag="ACHKUI_000013"
     CDS             9607..10887
                     /gene="kbaZ"
                     /locus_tag="ACHKUI_000013"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6596301.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKAAALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10910..11383
                     /gene="agaV"
                     /locus_tag="ACHKUI_000014"
     CDS             10910..11383
                     /gene="agaV"
                     /locus_tag="ACHKUI_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6596302.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11394..12173
                     /gene="agaW"
                     /locus_tag="ACHKUI_000015"
     CDS             11394..12173
                     /gene="agaW"
                     /locus_tag="ACHKUI_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6596303.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            12163..13041
                     /gene="agaE"
                     /locus_tag="ACHKUI_000016"
     CDS             12163..13041
                     /gene="agaE"
                     /locus_tag="ACHKUI_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6596304.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AITVVLGIVGKFCHFL"
     gene            13059..13493
                     /gene="agaF"
                     /locus_tag="ACHKUI_000017"
     CDS             13059..13493
                     /gene="agaF"
                     /locus_tag="ACHKUI_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6596305.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13490..14623
                     /gene="nagA"
                     /locus_tag="ACHKUI_000018"
     CDS             13490..14623
                     /gene="nagA"
                     /locus_tag="ACHKUI_000018"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6596306.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14973..16127
                     /gene="agaS"
                     /locus_tag="ACHKUI_000019"
     CDS             14973..16127
                     /gene="agaS"
                     /locus_tag="ACHKUI_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6596307.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNSF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQSAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            16140..17000
                     /gene="kbaY"
                     /locus_tag="ACHKUI_000020"
     CDS             16140..17000
                     /gene="kbaY"
                     /locus_tag="ACHKUI_000020"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6596308.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSMTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            17167..17643
                     /gene="agaB"
                     /locus_tag="ACHKUI_000021"
     CDS             17167..17643
                     /gene="agaB"
                     /locus_tag="ACHKUI_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6596309.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17682..18485
                     /gene="agaC"
                     /locus_tag="ACHKUI_000022"
                     /pseudo
     CDS             17682..18485
                     /gene="agaC"
                     /locus_tag="ACHKUI_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
     gene            18475..19266
                     /gene="agaD"
                     /locus_tag="ACHKUI_000023"
     CDS             18475..19266
                     /gene="agaD"
                     /locus_tag="ACHKUI_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6596310.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            19267..20034
                     /gene="agaI"
                     /locus_tag="ACHKUI_000024"
     CDS             19267..20034
                     /gene="agaI"
                     /locus_tag="ACHKUI_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO6596311.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQIENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(20084..20947)
                     /gene="rsmI"
                     /locus_tag="ACHKUI_000025"
     CDS             complement(20084..20947)
                     /gene="rsmI"
                     /locus_tag="ACHKUI_000025"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6596312.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            21012..23048
                     /gene="lpoA"
                     /locus_tag="ACHKUI_000026"
     CDS             21012..23048
                     /gene="lpoA"
                     /locus_tag="ACHKUI_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6596313.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQSQPQTVDDVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGPVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGSRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            23006..23401
                     /gene="yraN"
                     /locus_tag="ACHKUI_000027"
     CDS             23006..23401
                     /gene="yraN"
                     /locus_tag="ACHKUI_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6596314.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            23421..24011
                     /gene="diaA"
                     /locus_tag="ACHKUI_000028"
     CDS             23421..24011
                     /gene="diaA"
                     /locus_tag="ACHKUI_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6596315.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            24021..24596
                     /gene="dolP"
                     /locus_tag="ACHKUI_000029"
     CDS             24021..24596
                     /gene="dolP"
                     /locus_tag="ACHKUI_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6596316.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(24709..25749)
                     /gene="yraQ"
                     /locus_tag="ACHKUI_000030"
     CDS             complement(24709..25749)
                     /gene="yraQ"
                     /locus_tag="ACHKUI_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6596317.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMVYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(25822..26457)
                     /gene="yraR"
                     /locus_tag="ACHKUI_000031"
     CDS             complement(25822..26457)
                     /gene="yraR"
                     /locus_tag="ACHKUI_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6596318.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            26585..27103
                     /gene="yhbO"
                     /locus_tag="ACHKUI_000032"
     CDS             26585..27103
                     /gene="yhbO"
                     /locus_tag="ACHKUI_000032"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6596319.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(27083..27526)
                     /gene="yhbP"
                     /locus_tag="ACHKUI_000033"
     CDS             complement(27083..27526)
                     /gene="yhbP"
                     /locus_tag="ACHKUI_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6596320.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            27577..27879
                     /gene="yhbQ"
                     /locus_tag="ACHKUI_000034"
     CDS             27577..27879
                     /gene="yhbQ"
                     /locus_tag="ACHKUI_000034"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6596321.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAECAVFAELLNSLQTPEIKSD"
     gene            complement(27866..28369)
                     /gene="yhbS"
                     /locus_tag="ACHKUI_000035"
     CDS             complement(27866..28369)
                     /gene="yhbS"
                     /locus_tag="ACHKUI_000035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6596322.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(28363..28887)
                     /gene="ubiT"
                     /locus_tag="ACHKUI_000036"
     CDS             complement(28363..28887)
                     /gene="ubiT"
                     /locus_tag="ACHKUI_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6596323.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            29096..30091
                     /gene="ubiU"
                     /locus_tag="ACHKUI_000037"
     CDS             29096..30091
                     /gene="ubiU"
                     /locus_tag="ACHKUI_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6596324.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            30100..30978
                     /gene="ubiV"
                     /locus_tag="ACHKUI_000038"
     CDS             30100..30978
                     /gene="ubiV"
                     /locus_tag="ACHKUI_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6596325.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            31059..32066
                     /gene="yhbW"
                     /locus_tag="ACHKUI_000039"
     CDS             31059..32066
                     /gene="yhbW"
                     /locus_tag="ACHKUI_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO6596326.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKDELL
                     G"
     gene            complement(32184..33428)
                     /gene="mtr"
                     /locus_tag="ACHKUI_000040"
     CDS             complement(32184..33428)
                     /gene="mtr"
                     /locus_tag="ACHKUI_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6596327.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(33582..35471)
                     /gene="deaD"
                     /locus_tag="ACHKUI_000041"
     CDS             complement(33582..35471)
                     /gene="deaD"
                     /locus_tag="ACHKUI_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6596328.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLTAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(35464..35544)
                     /gene="yrbN"
                     /locus_tag="ACHKUI_000042"
     CDS             complement(35464..35544)
                     /gene="yrbN"
                     /locus_tag="ACHKUI_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6596329.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(35651..36535)
                     /gene="nlpI"
                     /locus_tag="ACHKUI_000043"
     CDS             complement(35651..36535)
                     /gene="nlpI"
                     /locus_tag="ACHKUI_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6596330.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLACYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(36644..38779)
                     /gene="pnp"
                     /locus_tag="ACHKUI_000044"
     CDS             complement(36644..38779)
                     /gene="pnp"
                     /locus_tag="ACHKUI_000044"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6596331.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATGGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(39026..39295)
                     /gene="rpsO"
                     /locus_tag="ACHKUI_000045"
     CDS             complement(39026..39295)
                     /gene="rpsO"
                     /locus_tag="ACHKUI_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6596332.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(39444..40388)
                     /gene="truB"
                     /locus_tag="ACHKUI_000046"
     CDS             complement(39444..40388)
                     /gene="truB"
                     /locus_tag="ACHKUI_000046"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6596333.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(40388..40789)
                     /gene="rbfA"
                     /locus_tag="ACHKUI_000047"
     CDS             complement(40388..40789)
                     /gene="rbfA"
                     /locus_tag="ACHKUI_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6596334.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(40953..43625)
                     /gene="infB"
                     /locus_tag="ACHKUI_000048"
     CDS             complement(40953..43625)
                     /gene="infB"
                     /locus_tag="ACHKUI_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6596335.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(43650..45137)
                     /gene="nusA"
                     /locus_tag="ACHKUI_000049"
     CDS             complement(43650..45137)
                     /gene="nusA"
                     /locus_tag="ACHKUI_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6596336.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(45165..45617)
                     /gene="rimP"
                     /locus_tag="ACHKUI_000050"
     CDS             complement(45165..45617)
                     /gene="rimP"
                     /locus_tag="ACHKUI_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6596337.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(45824..45900)
                     /locus_tag="ACHKUI_000051"
     tRNA            complement(45824..45900)
                     /locus_tag="ACHKUI_000051"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45864..45866),aa:Met,seq:cat)
     gene            46248..47591
                     /gene="argG"
                     /locus_tag="ACHKUI_000052"
     CDS             46248..47591
                     /gene="argG"
                     /locus_tag="ACHKUI_000052"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6596338.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(47599..49224)
                     /gene="yhbX"
                     /locus_tag="ACHKUI_000053"
     CDS             complement(47599..49224)
                     /gene="yhbX"
                     /locus_tag="ACHKUI_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6596339.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLIAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(49681..49767)
                     /locus_tag="ACHKUI_000054"
     tRNA            complement(49681..49767)
                     /locus_tag="ACHKUI_000054"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49731..49733),aa:Leu,seq:gag)
     gene            complement(49782..50114)
                     /gene="secG"
                     /locus_tag="ACHKUI_000055"
     CDS             complement(49782..50114)
                     /gene="secG"
                     /locus_tag="ACHKUI_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6596340.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(50342..51679)
                     /gene="glmM"
                     /locus_tag="ACHKUI_000056"
     CDS             complement(50342..51679)
                     /gene="glmM"
                     /locus_tag="ACHKUI_000056"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6596341.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(51672..52520)
                     /gene="folP"
                     /locus_tag="ACHKUI_000057"
     CDS             complement(51672..52520)
                     /gene="folP"
                     /locus_tag="ACHKUI_000057"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6596342.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(52610..54544)
                     /gene="ftsH"
                     /locus_tag="ACHKUI_000058"
     CDS             complement(52610..54544)
                     /gene="ftsH"
                     /locus_tag="ACHKUI_000058"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6596343.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(54644..55273)
                     /gene="rlmE"
                     /locus_tag="ACHKUI_000059"
     CDS             complement(54644..55273)
                     /gene="rlmE"
                     /locus_tag="ACHKUI_000059"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6596344.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVNQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            55399..55692
                     /gene="yhbY"
                     /locus_tag="ACHKUI_000060"
     CDS             55399..55692
                     /gene="yhbY"
                     /locus_tag="ACHKUI_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6596345.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(55848..56324)
                     /gene="greA"
                     /locus_tag="ACHKUI_000061"
     CDS             complement(55848..56324)
                     /gene="greA"
                     /locus_tag="ACHKUI_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6596346.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            56572..58005
                     /gene="dacB"
                     /locus_tag="ACHKUI_000062"
     CDS             56572..58005
                     /gene="dacB"
                     /locus_tag="ACHKUI_000062"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6596347.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(58046..59218)
                     /gene="cgtA"
                     /locus_tag="ACHKUI_000063"
     CDS             complement(58046..59218)
                     /gene="cgtA"
                     /locus_tag="ACHKUI_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6596348.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(59234..60199)
                     /gene="yhbE"
                     /locus_tag="ACHKUI_000064"
     CDS             complement(59234..60199)
                     /gene="yhbE"
                     /locus_tag="ACHKUI_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6596349.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLADPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(60326..60583)
                     /gene="rpmA"
                     /locus_tag="ACHKUI_000065"
     CDS             complement(60326..60583)
                     /gene="rpmA"
                     /locus_tag="ACHKUI_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6596350.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(60604..60915)
                     /gene="rplU"
                     /locus_tag="ACHKUI_000066"
     CDS             complement(60604..60915)
                     /gene="rplU"
                     /locus_tag="ACHKUI_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6596351.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            61174..62145
                     /gene="ispB"
                     /locus_tag="ACHKUI_000067"
     CDS             61174..62145
                     /gene="ispB"
                     /locus_tag="ACHKUI_000067"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6596352.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALVGLAHIAVQRDR"
     gene            62378..62656
                     /gene="sfsB"
                     /locus_tag="ACHKUI_000068"
     CDS             62378..62656
                     /gene="sfsB"
                     /locus_tag="ACHKUI_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6596353.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(62704..63963)
                     /gene="murA"
                     /locus_tag="ACHKUI_000069"
     CDS             complement(62704..63963)
                     /gene="murA"
                     /locus_tag="ACHKUI_000069"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6596354.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(64018..64272)
                     /gene="ibaG"
                     /locus_tag="ACHKUI_000070"
     CDS             complement(64018..64272)
                     /gene="ibaG"
                     /locus_tag="ACHKUI_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6596355.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(64432..64725)
                     /gene="mlaB"
                     /locus_tag="ACHKUI_000071"
     CDS             complement(64432..64725)
                     /gene="mlaB"
                     /locus_tag="ACHKUI_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6596356.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(64725..65360)
                     /gene="mlaC"
                     /locus_tag="ACHKUI_000072"
     CDS             complement(64725..65360)
                     /gene="mlaC"
                     /locus_tag="ACHKUI_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6596357.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(65379..65930)
                     /gene="mlaD"
                     /locus_tag="ACHKUI_000073"
     CDS             complement(65379..65930)
                     /gene="mlaD"
                     /locus_tag="ACHKUI_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6596358.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(65935..66717)
                     /gene="mlaE"
                     /locus_tag="ACHKUI_000074"
     CDS             complement(65935..66717)
                     /gene="mlaE"
                     /locus_tag="ACHKUI_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6596359.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(66725..67534)
                     /gene="mlaF"
                     /locus_tag="ACHKUI_000075"
     CDS             complement(66725..67534)
                     /gene="mlaF"
                     /locus_tag="ACHKUI_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6596360.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVSRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            67744..68721
                     /gene="yrbG"
                     /locus_tag="ACHKUI_000076"
     CDS             67744..68721
                     /gene="yrbG"
                     /locus_tag="ACHKUI_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6596361.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVTTRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            68735..69721
                     /gene="kdsD"
                     /locus_tag="ACHKUI_000077"
     CDS             68735..69721
                     /gene="kdsD"
                     /locus_tag="ACHKUI_000077"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6596362.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            69742..70308
                     /gene="kdsC"
                     /locus_tag="ACHKUI_000078"
     CDS             69742..70308
                     /gene="kdsC"
                     /locus_tag="ACHKUI_000078"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6596363.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTHIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            70305..70880
                     /gene="lptC"
                     /locus_tag="ACHKUI_000079"
     CDS             70305..70880
                     /gene="lptC"
                     /locus_tag="ACHKUI_000079"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6596364.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRNKNAELIEKVRTSYEIQNKQTQP"
     gene            70849..71406
                     /gene="lptA"
                     /locus_tag="ACHKUI_000080"
     CDS             70849..71406
                     /gene="lptA"
                     /locus_tag="ACHKUI_000080"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6596365.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            71413..72138
                     /gene="lptB"
                     /locus_tag="ACHKUI_000081"
     CDS             71413..72138
                     /gene="lptB"
                     /locus_tag="ACHKUI_000081"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6596366.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            72186..73619
                     /gene="rpoN"
                     /locus_tag="ACHKUI_000082"
     CDS             72186..73619
                     /gene="rpoN"
                     /locus_tag="ACHKUI_000082"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6596367.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            73642..73929
                     /gene="hpf"
                     /locus_tag="ACHKUI_000083"
     CDS             73642..73929
                     /gene="hpf"
                     /locus_tag="ACHKUI_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6596368.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            74047..74538
                     /gene="ptsN"
                     /locus_tag="ACHKUI_000084"
     CDS             74047..74538
                     /gene="ptsN"
                     /locus_tag="ACHKUI_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6596369.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            74584..75438
                     /gene="rapZ"
                     /locus_tag="ACHKUI_000085"
     CDS             74584..75438
                     /gene="rapZ"
                     /locus_tag="ACHKUI_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6596370.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            75435..75707
                     /gene="npr"
                     /locus_tag="ACHKUI_000086"
     CDS             75435..75707
                     /gene="npr"
                     /locus_tag="ACHKUI_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6596371.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            75921..76553
                     /gene="yrbL"
                     /locus_tag="ACHKUI_000087"
     CDS             75921..76553
                     /gene="yrbL"
                     /locus_tag="ACHKUI_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6596372.1"
                     /translation="MIRLSEQTPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(76550..77278)
                     /gene="mtgA"
                     /locus_tag="ACHKUI_000088"
     CDS             complement(76550..77278)
                     /gene="mtgA"
                     /locus_tag="ACHKUI_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6596373.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(77275..77928)
                     /gene="elbB"
                     /locus_tag="ACHKUI_000089"
     CDS             complement(77275..77928)
                     /gene="elbB"
                     /locus_tag="ACHKUI_000089"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6596374.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(78158..80494)
                     /gene="arcB"
                     /locus_tag="ACHKUI_000090"
     CDS             complement(78158..80494)
                     /gene="arcB"
                     /locus_tag="ACHKUI_000090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6596375.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(80590..81519)
                     /gene="yhcC"
                     /locus_tag="ACHKUI_000091"
     CDS             complement(80590..81519)
                     /gene="yhcC"
                     /locus_tag="ACHKUI_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6596376.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            82194..86654
                     /gene="gltB"
                     /locus_tag="ACHKUI_000092"
     CDS             82194..86654
                     /gene="gltB"
                     /locus_tag="ACHKUI_000092"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6596377.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEK
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            86667..88085
                     /gene="gltD"
                     /locus_tag="ACHKUI_000093"
     CDS             86667..88085
                     /gene="gltD"
                     /locus_tag="ACHKUI_000093"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6596378.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNIQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(88153..88617)
                     /gene="nanQ"
                     /locus_tag="ACHKUI_000094"
     CDS             complement(88153..88617)
                     /gene="nanQ"
                     /locus_tag="ACHKUI_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6596379.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKIKADLMA"
     gene            complement(88614..89489)
                     /gene="nanK"
                     /locus_tag="ACHKUI_000095"
     CDS             complement(88614..89489)
                     /gene="nanK"
                     /locus_tag="ACHKUI_000095"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6596380.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(89486..90175)
                     /gene="nanE"
                     /locus_tag="ACHKUI_000096"
     CDS             complement(89486..90175)
                     /gene="nanE"
                     /locus_tag="ACHKUI_000096"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6596381.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(90223..91713)
                     /gene="nanT"
                     /locus_tag="ACHKUI_000097"
     CDS             complement(90223..91713)
                     /gene="nanT"
                     /locus_tag="ACHKUI_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6596382.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNNLVKTKS"
     gene            complement(91822..92715)
                     /gene="nanA"
                     /locus_tag="ACHKUI_000098"
     CDS             complement(91822..92715)
                     /gene="nanA"
                     /locus_tag="ACHKUI_000098"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6596383.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(92837..93628)
                     /gene="nanR"
                     /locus_tag="ACHKUI_000099"
     CDS             complement(92837..93628)
                     /gene="nanR"
                     /locus_tag="ACHKUI_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6596384.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(93736..94233)
                     /gene="sspB"
                     /locus_tag="ACHKUI_000100"
     CDS             complement(93736..94233)
                     /gene="sspB"
                     /locus_tag="ACHKUI_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6596385.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(94239..94877)
                     /gene="sspA"
                     /locus_tag="ACHKUI_000101"
     CDS             complement(94239..94877)
                     /gene="sspA"
                     /locus_tag="ACHKUI_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6596386.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(95271..95663)
                     /gene="rpsI"
                     /locus_tag="ACHKUI_000102"
     CDS             complement(95271..95663)
                     /gene="rpsI"
                     /locus_tag="ACHKUI_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6596387.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(95679..96107)
                     /gene="rplM"
                     /locus_tag="ACHKUI_000103"
     CDS             complement(95679..96107)
                     /gene="rplM"
                     /locus_tag="ACHKUI_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6596388.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(96327..97454)
                     /gene="zapE"
                     /locus_tag="ACHKUI_000104"
     CDS             complement(96327..97454)
                     /gene="zapE"
                     /locus_tag="ACHKUI_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6596389.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRFFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            97648..98046
                     /gene="zapG"
                     /locus_tag="ACHKUI_000105"
     CDS             97648..98046
                     /gene="zapG"
                     /locus_tag="ACHKUI_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6596390.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            98200..99567
                     /gene="degQ"
                     /locus_tag="ACHKUI_000106"
     CDS             98200..99567
                     /gene="degQ"
                     /locus_tag="ACHKUI_000106"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6596391.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            99657..100724
                     /gene="degS"
                     /locus_tag="ACHKUI_000107"
     CDS             99657..100724
                     /gene="degS"
                     /locus_tag="ACHKUI_000107"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6596392.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLILQLTIQEYPATN"
     gene            complement(100787..101725)
                     /gene="mdh"
                     /locus_tag="ACHKUI_000108"
     CDS             complement(100787..101725)
                     /gene="mdh"
                     /locus_tag="ACHKUI_000108"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6596393.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            102160..102630
                     /gene="argR"
                     /locus_tag="ACHKUI_000109"
     CDS             102160..102630
                     /gene="argR"
                     /locus_tag="ACHKUI_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6596394.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
     gene            102994..103257
                     /gene="yhcN"
                     /locus_tag="ACHKUI_000110"
     CDS             102994..103257
                     /gene="yhcN"
                     /locus_tag="ACHKUI_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6596395.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(103312..103584)
                     /gene="yhcO"
                     /locus_tag="ACHKUI_000111"
     CDS             complement(103312..103584)
                     /gene="yhcO"
                     /locus_tag="ACHKUI_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6596396.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(103676..105643)
                     /gene="aaeB"
                     /locus_tag="ACHKUI_000112"
     CDS             complement(103676..105643)
                     /gene="aaeB"
                     /locus_tag="ACHKUI_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6596397.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(105649..106581)
                     /gene="aaeA"
                     /locus_tag="ACHKUI_000113"
     CDS             complement(105649..106581)
                     /gene="aaeA"
                     /locus_tag="ACHKUI_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6596398.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(106589..106792)
                     /gene="aaeX"
                     /locus_tag="ACHKUI_000114"
     CDS             complement(106589..106792)
                     /gene="aaeX"
                     /locus_tag="ACHKUI_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6596399.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            106975..107904
                     /gene="aaeR"
                     /locus_tag="ACHKUI_000115"
     CDS             106975..107904
                     /gene="aaeR"
                     /locus_tag="ACHKUI_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6596400.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(108032..109477)
                     /gene="tldD"
                     /locus_tag="ACHKUI_000116"
     CDS             complement(108032..109477)
                     /gene="tldD"
                     /locus_tag="ACHKUI_000116"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6596401.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(109633..113433)
                     /gene="yhdP"
                     /locus_tag="ACHKUI_000117"
     CDS             complement(109633..113433)
                     /gene="yhdP"
                     /locus_tag="ACHKUI_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6596402.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLLAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELKLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKIGFLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPVRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(113501..114970)
                     /gene="rng"
                     /locus_tag="ACHKUI_000118"
     CDS             complement(113501..114970)
                     /gene="rng"
                     /locus_tag="ACHKUI_000118"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6596403.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(114960..115553)
                     /gene="yhdE"
                     /locus_tag="ACHKUI_000119"
     CDS             complement(114960..115553)
                     /gene="yhdE"
                     /locus_tag="ACHKUI_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6596404.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTEQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(115562..116050)
                     /gene="mreD"
                     /locus_tag="ACHKUI_000120"
     CDS             complement(115562..116050)
                     /gene="mreD"
                     /locus_tag="ACHKUI_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6596405.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(116053..117153)
                     /gene="mreC"
                     /locus_tag="ACHKUI_000121"
     CDS             complement(116053..117153)
                     /gene="mreC"
                     /locus_tag="ACHKUI_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6596406.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGG"
     gene            complement(117219..118262)
                     /gene="mreB"
                     /locus_tag="ACHKUI_000122"
     CDS             complement(117219..118262)
                     /gene="mreB"
                     /locus_tag="ACHKUI_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6596407.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(118567..120507)
                     /gene="csrD"
                     /locus_tag="ACHKUI_000123"
     CDS             complement(118567..120507)
                     /gene="csrD"
                     /locus_tag="ACHKUI_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6596408.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTYLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            120659..121633
                     /gene="yhdH"
                     /locus_tag="ACHKUI_000124"
     CDS             120659..121633
                     /gene="yhdH"
                     /locus_tag="ACHKUI_000124"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6596409.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            121819..122206
                     /locus_tag="ACHKUI_000125"
                     /pseudo
     CDS             121819..122206
                     /locus_tag="ACHKUI_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            122611..123081
                     /gene="accB"
                     /locus_tag="ACHKUI_000126"
     CDS             122611..123081
                     /gene="accB"
                     /locus_tag="ACHKUI_000126"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6596410.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            123092..124441
                     /gene="accC"
                     /locus_tag="ACHKUI_000127"
     CDS             123092..124441
                     /gene="accC"
                     /locus_tag="ACHKUI_000127"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6596411.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(124533..125579)
                     /locus_tag="ACHKUI_000128"
     CDS             complement(124533..125579)
                     /locus_tag="ACHKUI_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO6596412.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDNIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTL
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(125576..126538)
                     /locus_tag="ACHKUI_000129"
     CDS             complement(125576..126538)
                     /locus_tag="ACHKUI_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6596413.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKENGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIETFIQRHDMSIREAAQ"
     gene            complement(126560..127549)
                     /locus_tag="ACHKUI_000130"
     CDS             complement(126560..127549)
                     /locus_tag="ACHKUI_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6596414.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(127550..129049)
                     /locus_tag="ACHKUI_000131"
     CDS             complement(127550..129049)
                     /locus_tag="ACHKUI_000131"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6596415.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKTDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(129110..>129951)
                     /locus_tag="ACHKUI_000132"
     CDS             complement(129110..>129951)
                     /locus_tag="ACHKUI_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6596416.1"
                     /translation="FSQSSLAKEYKIGASLLTQQHPFYIDLANAMKEEAKKDNVNLSV
                     SIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADKAGIPVITVDVAAEG
                     VPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQSVVARVDGFKKGLSDTP
                     DIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKI
                     IGFDGMKEARDAVDSEKTFAAVIRQYPDQMEAKAIDAAVDHLNGKPVEKMIPVQPGVY
                     TGK"
BASE COUNT        32011 a        34924 c        32091 g        30925 t
ORIGIN      
        1 aaaaaaaatg acggggataa ccatatttta tggcgataac atcattcgtt atgtggttaa
       61 tacaaaaaag gggctgagac tatatttcaa gcaattaccg gataattacc aggcaaaatt
      121 tgagttaaat ttaatgagta agttctcaaa ttttattata aacaaaccat tttcagcgat
      181 aaataatgcg gcacgtcaca ttttttcacg ctatttgttg gagaacaaac atttatttta
      241 tcaatatttt aaaatttcga atacaggtat tgatcatctc gaacaattga ttaacgtcaa
      301 ctttttctct tctgacagga cgtcattttg cgaatgcaat cgttttccat aaattcttct
      361 cccctcatag gcaacgaata gcattttgtg ttgaggatca caaaacgaat aattgctggt
      421 cgccgcgata aggtcagacg aagtcaacaa ggaggatttt cgctgtgctt tacatcagcg
      481 taggccatag aatgttgctt cgttgactgt aatgccgggt aaatgagcat tttttgatag
      541 tgcgaaagaa ccctgcgagg aaaataagca tctattattg tttgtcatga tgacaaggca
      601 ggcatcaatg aaaggcttct caattgcaca tatttttcac ccttcaatgc acgcagtggt
      661 taacaatcac aatagaaata ttgattattg gacggtaaaa atttaggtta taacgcccaa
      721 atgattcaac gatcggacaa tacctgcgaa cttacaatta atgagagaaa tatatgtata
      781 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct gttattaata
      841 ccgtagatat tcatcttacc ctgggcacca aaagcaaaaa cccgccgaag cgggttttta
      901 cgtaaatcag gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag
      961 gccagcaatc gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat
     1021 gaacccctat ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg
     1081 cgcggtgcgc tcttaccgca ccctttcacc cttacctgat cccgcttacg cgggccatcg
     1141 gcggtttgct ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct
     1201 gccctatgga gcccggactt tcctcccctc cgcccgtctc ccccgaagag gacgacgacg
     1261 aagcagcgac tgtctggtca gcttcggcgc gcagtataga gggtttacgc gcccttgtca
     1321 ccccgcgttg cgcattccaa tcgccagtgt cgcggcgata ttacgtgaag cacggcagat
     1381 attgtcataa gctccgcgga gtgcttcgtc taacgtacca atgctggtca gtacgctgaa
     1441 gaccgcatca atgccatgct gatgtacaac gccaacatca ttggtcaggc tacccgcaat
     1501 gccaatcacc ggtttatggt acttcttcgc cacgtttgcg acaccaatcg gcaccttgcc
     1561 gtgaatactc tggctatcaa tacgcccttc accagtgatc accagcgtac aatcgtgaat
     1621 atgttcctcc agattcagcg ccgtcgtgac gatttcaata ccacttttca gttctgcacc
     1681 aagaaacgcc atcagcgccg cgcccatacc gcccgcagct cctgcgccgg ggacatcttt
     1741 cacatcaaca tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattatccag
     1801 ctcgacaatc atcgcctcac tggctccctt ttgtgggcca aagatgcgcg aagcaccact
     1861 gtcgccgact aacgggttgg tgacatcaca agcgacgcgg atgacacaat cttttaagcg
     1921 cggatcgaga ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc
     1981 aatttcattg ccgttggcat cgcataattt cgcccccagc gcctgtacca tgcctgcgcc
     2041 gccgtcgttg gtggcgctgc cgccaatgcc gataataatg tttgtcgcac cgctctccag
     2101 cgcctgcagg attaactcac cagtaccgcg tgaagtggtc acgagtggat cgcgtttttc
     2161 cgcaggtacc agttccagcc cactggccgc cgccatttcg ataaacgcgg ttttgccatc
     2221 gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg
     2281 acgttcagcc ccctgggtgg ctgcaatcat cgcttccacc gttccttcac caccgtcggc
     2341 aaccggaaca gaaacatact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc
     2401 ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt
     2461 catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc
     2521 caggcccgga ttgcaatacg ccatccgggc acgacgtcat tagcgagtaa cttcgacttt
     2581 cgccagtttt tcgtagtagc acgccagggc gctatgatcc gccattccta aaccatctgc
     2641 acgcagtgcc tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc
     2701 gtgagaagta tccagcgcat tcgccagatc cttaatatgc agatcaatac ggaagcccgg
     2761 tttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag
     2821 cccaccgcga attgcctgat acaccagatc cgggttaacg cccgctttgg ttgccagcgt
     2881 taacgcttct gacatcgcag caatattcag cgccacaatg acctgatttg ccagtttggt
     2941 gacgttacct gcaccgattt ccccggtatg caccacggaa cccgccatcg ctttcatcaa
     3001 atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc
     3061 gatggctttc ggttcaccgc cgctcaccgg ggcgtccagc atatcaatgc ctttcgcttt
     3121 cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac
     3181 cgtacctggc ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg
     3241 aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt
     3301 agaggctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc
     3361 aaccaccagc gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc
     3421 caggccaata aaaccaactt tcatagtcat atcaatcatc tctcttgttg cggtggtggt
     3481 tattttttaa aggtatcagc cagtttctga gtggcagagc ggaagatgcc gagatcgctg
     3541 ccgacagcca caaacgtcgc accccattcc agataacgac gcgcatcagc ctcgactggt
     3601 gcgaggatgc cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg ctgaattgct
     3661 ttttgtacat ccgggtgcga ggcattgccg agatggccta atgccgcagc cagatcgctg
     3721 gggccgacga agatgccgtc tacaccttcg gtagcggcaa tagcatcgac gttatctacg
     3781 ccctgctgac tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa
     3841 tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc
     3901 ggtgggtaac gagttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga
     3961 atcaggaagt tatagaaacc gatatccaga agacgcttaa taattaccgg ctcgttggtc
     4021 ggcactcgca ctactggcgc gctggcgctg cctttcaggg ccattaactg cggaataaac
     4081 gtggagatat cgtttggcgc atgttcacca tccagcacca gccagtcaaa cccagccaaa
     4141 ccaagaactt cagtgctaat cgggttagag agagctgacc agcaaccaat ttgtacctgt
     4201 ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc
     4261 tttgcttatt tctgcaattc catacgttta atgtcgccga ctacgaagag gtagcagacc
     4321 atcgccatca gcgctgaaca tcccacaaaa accagtgctg cattgaagga gtgcagttca
     4381 cttaccaggt agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta
     4441 aagacgccgc cgcagaggcc aacaatctct ttcggcgcgg tgtcagaaat cactggccag
     4501 cccagcgcac caaatccttt accaaagaaa gccagcgcca tcagcatgac caccagcgtg
     4561 gtgttgttgg tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatt
     4621 ggtagcttac gtgccagggt cagggacaaa ccgcgtttga tcagataatc cgagaagaca
     4681 cctcccagca cgccgcccgc aaagccacac agtgccggaa tcgaggcgac cagacccact
     4741 ttcagaatcg acatgccttt ttcctgcacc agataaattg ggaaccaggt gaggaagaac
     4801 caggtgatgg tgttgataaa atattgtccg aaaaatacgc ccagcatcat gcggttagag
     4861 agcaattgct tgatgtaatg cagtttgggt ccgcttgctg ccgcactgcc cggctttttg
     4921 tggtccatat cgaccaccgc gccattttcc gagataaact tcagctcttc cgcagacata
     4981 cgtgggtgat ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacca
     5041 atcaccccca taacggtaaa gacgtgctcc cagccccagg cgaaagtcag ccagccaagc
     5101 agcggcgaaa agagcgccaa cgagaaatat tgcgccgagt taaagatggc ggaggcagta
     5161 ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatggc
     5221 gcttccgaga accccagcat aaagcgcata aagaacatgg agatcccagc ccaggccagc
     5281 ggaaacatat cgacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtaa
     5341 gtgtaaactt tcttcgagcc aaacttatca agcagccagc cgccgggaat ttgcatcagc
     5401 aagtaggccc agccaaaagc agagaagatg taacccatcg aaaccgcact taactgcaac
     5461 tcttttgcca cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg
     5521 gtaacaataa aaataatcag taatattaaa tagcgggtat gcacgccttt ctttttttcg
     5581 tcaacggtgt ccagcatcat tttatttacc tcgggtactt atgctgattt ttattattat
     5641 ggggaaggtg ttatttatga gtttcattta tgccgttacg acaatgaact cgggaattag
     5701 tataagcagc gcgagaataa taatcattgt gcaaatgcta atttaattaa tactatttaa
     5761 atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt gcgctttacc
     5821 ttgccgggag gtactgcaat ccctgaaatg attgacattg atcacatttc tgcatttaaa
     5881 ctcctgacat tcttatttca cccaatgaag ttatttattt ttaaatgaga ccaggtcctc
     5941 attttaatag tccctggctg gagaatattg cacaatggcc aacatcaaaa tcagacaaga
     6001 aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa ttattgttaa
     6061 tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa ttgaacatat
     6121 tccccagggg cataaagtcg cattgctgga cattccggct aatggtgaaa ttattcgtta
     6181 tggcgaggtg attggttacg ccgtgcgcgc aatcccacgc ggaagctgga tcgacgaatc
     6241 aatggttgta ctaccggaag cgccgccgtt acatacgctg ccactggcaa ccaaagtccc
     6301 ggaaccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg ccgatggcag
     6361 cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg caggcgtggt
     6421 ggactacgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga acgtcgatgg
     6481 cgtggtgggg ctgaatcatt tgtacggttg tggcgtggcg attaacgcac ccgcggcagt
     6541 tgtacctatt cgtaccattc acaatatttc gctgaaccct aactttggcg gcgaagtaat
     6601 ggtgattggt ctgggttgtg aaaagttgca gcctgaacgc ctactgactg gaacgaatga
     6661 tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt
     6721 cggttttcag tccatggtcg aggatatttt gcaggtcgcc gaatgccatc tacaaaaact
     6781 gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg
     6841 tggcagcgat gcgttttctg gcgtaacggc aaacccggcg gttgggtatg cgtctgatct
     6901 attggtgcgc tgcggcgcaa cggtgatgtt ttcagaagtc acagaagtgc gtgacgcgat
     6961 acatttgctg acaccacgcg ctgtgaacga agaggtcggc aaacggctgc tggaggagat
     7021 ggagtggtac gataactatc tcaatatggg aaaaaccgat cgcagcgcca acccttcgcc
     7081 ggggaacaag aaaggcggtc tggcaaatgt ggtggagaag gcactcggct ccattgctaa
     7141 atcgggtaaa agtgcaattg ttgaagtgct gtcgcccggc caacgcccga ctaaacgcgg
     7201 attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac aggtggcttc
     7261 gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt
     7321 acccgtcatt aaaatggcga cccgcaccga gctggcgaac cgctggtttg atttaatgga
     7381 tattaacgcg ggcactatcg ctaccggcga agaaaccatt gaagaagtgg gctggaagtt
     7441 gtttcacttt attctcgacg tcgccagcgg gaagaagaaa actttctcgg atcaatgggg
     7501 actgcataac cagctggcgg tgtttaaccc ggcaccggtg acctgatttc ttttggaacg
     7561 tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagtcca cacatccatt
     7621 aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa caggctgtaa
     7681 aaggacagta aataatgccc gctaatgctc gctctaacgc tgtactgacc actgaatcaa
     7741 aggtcactat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga
     7801 agcctggcct ggacagcatt cattacgaaa ttctgcctgg tgggcaagta tttatgtgcc
     7861 gactgggaga tgaacaggag gatcatacta tgaatgcatt tttgcgtttt ctggatgcag
     7921 atatccagaa caacccgcaa aaaactcgtc cattcgacat tcaacaagga aagaaacttg
     7981 tcgctggcat ggacgtcaac attgacgatg agattggcga cgacgaataa tggattttcc
     8041 acaaagggtt aatggttggg cgctatatgc tcatccctgt tttcaggaaa cctacgatgc
     8101 tttagttgcc gaagtcgagg cattaaaggg aaaagatcct gaaaattatc agagaaaagc
     8161 tgccacaaag ttattggcgg tggtccataa agtgattgag gagcatatca cggtcaatcc
     8221 atcatcaccg gcattccgtc atggcaagtc gttaggctct gggaaaaata aagactggtc
     8281 acgggtaaaa tttggcgctg gtcgctatcg tctcttcttt cgttacagtg aaaaagagaa
     8341 agtcatcatt ctgggatgga tgaacgatga aaacacgctg cgcacctacg gtaaaaaaac
     8401 agatgcctat accgtattca gtaaaatgtt aaaaagagga catcctcctg ccgactggga
     8461 aagcctcacc caagagacag aagaaaacca ttgatggtgt tcacaatgcc cgccgaacgc
     8521 attggtgttg accagcgggc atcctttctc attccccgac cagaatcact tcaaccccag
     8581 cctttctcag tccttccaga ctatccgcag gaatgccttc atcaacaatg atcatgtcga
     8641 tacgttgagt atcaatgatc ttatgtaaac tagaacgatt gaacttactg gaatcggtga
     8701 ccacgatgat ccgttccgca acttcgcaca tccgacggtt taaacgggct tcatcttcat
     8761 tatgcgtgct gacgccgcgc tccagatcga tcgcatctac accaagaaac agcatatcga
     8821 agtggtaatt ttgcagcgat tgctcagcct gatcaccgta aaaagattgc gactgacggc
     8881 gcaaatgccc cccggtcatc agcagctcaa cgccttccgc ttccagcaac gcattagcca
     8941 cgttcatacc gttggtcatc gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct
     9001 caaaagtggt ggtcccggag tcgaggatca cccgatgacc aggctgaatc aactcaacgg
     9061 cagctttcgc aacgctgcgt ttcatcgcgg tgttcagcgc gcttttatct tccactgatg
     9121 gctcgactga cggcgttgtg ctatcgcaga tcaacgcgcc accataggca cgcacagcga
     9181 tcccctgctt ttccagaaac gccagatcgt tgcggatcgt cacagtagat acaccataca
     9241 acgccgatag atcattaact tgcacacttc cttgctggcg caggcgttga atgatctgct
     9301 ctcgtcgctc actggtgcct gtcactcgct tctcacctga agcgtcggta ttactcatag
     9361 taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc tattaacgcc tttctattaa
     9421 gcaaatgcaa gcccacctta cccattggcg caagctactc tcgtttcagt ggctttcatt
     9481 atgtttcttt tgtgaatcag atcagaaaac tattatcttt cgttttattt ttatctcacc
     9541 atgacgcagt atcaactgaa acaaaacgaa agattaatgt cgcagtaatc tgaactggag
     9601 aggaaagtga aacatctgac agaaatggtg agacagcaca aagcgggcaa aacaaatggg
     9661 atttatgccg tttgttccgc acatccgctg gtgctggaag ctgcaatccg ctacgccagt
     9721 gcaaaccaaa cgccgttact gattgaagca acctccaatc aggtggacca gttcggcggt
     9781 tataccggaa tgacgcccgc cgattttcgc ggctttgttt gtcagctcgc cgactcgttg
     9841 aatttcccgc aggatgcgct gattctgggt ggcgaccatc tggggccaaa ccgctggcaa
     9901 aacctgcctg ccgctcaggc aatggccaat gccgatgatt tgattaaaag ctacgtggcg
     9961 gcaggattca aaaaaattca ccttgattgc agcatgtcct gtcaggacga tccgattccc
    10021 ttaactgatg acatcgtggc tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc
    10081 tgtcgtgaac actttggcga agccgatctg gagtatgtca ttggtaccga agtgccggta
    10141 cctggcggcg cgcatgaaac cttaagcgag ctggcggtca ccacgccgga tgccgcccgc
    10201 gccacgctgg aagcccatcg tcacgccttt gaaaagcaag gtctgaatgc catctggcca
    10261 cgcatcattg ccctggtggt tcaacccggc gtcgaattcg atcacaccaa cgttattgat
    10321 tatcagccag ccaaagccgc tgccttaagc cagatggtcg aaaactacga aacgctgatt
    10381 ttcgaagcgc actctaccga ttatcaaaca ccgcaatcgc tgcgccagct ggtgattgac
    10441 cactttgcca ttctgaaagt tggcccggcg ctgacctttg ctctgcgtga agctctgttc
    10501 tctctggcgg cgattgaaga agaactggtg ccagcaaaag cctgttctgg tctgcgtcag
    10561 gtgctggaaa acgtgatgct cgaccgcccg gaatactggc aaagccacta ccacggtgac
    10621 ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg atcgcgtgcg ctattactgg
    10681 ccggacagcc agattgatga cgctttcgct catctggttc gtaatctggc ggattcacca
    10741 attccgctgc cgctgatcag tcagtatctg ccgctgcagt acgtgaaagt tcgctccggc
    10801 gagctgcagc caacgccacg ggaactcatt atcaaccata ttcaggacat cctggcgcag
    10861 taccacacag cctgtgaagg ccaataagca aaacaaagag gaacacgcta tgccaaatat
    10921 tgttttaagt cggattgatg aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg
    10981 atttgcgggg gcaaatctgg tgctggtagc caacgatgag gttgccgaag atctggtaca
    11041 acaaaacctg atggaaatgg tactggcaga agggatcgcc gtacgtttct ggacgctgca
    11101 aaaagtcatc gacaacattc atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa
    11161 aacaccagcc gatttcctga cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt
    11221 tggcaatatg cactacgcca atggcaagca acaaatcgcc aaaacggttt ctgtggatgc
    11281 gggtgatatc gcagcattta acgacctgaa agccgctggg gtggaatgct tcgttcaggg
    11341 cgtcccgaca gagcctgctg tagacctctt taaattactt tgagggattc atcatggaaa
    11401 tcagtctgtt gcaggcattt gcgttgggca ttatcgcctt tatcgctggc ctggatatgt
    11461 ttaacggctt aacccatatg caccgcccgg tggttctcgg cccgttggtc gggctggtac
    11521 ttggcgatct gcataccggt attttaaccg gcggtacgct ggaactggtg tggatggggc
    11581 tggcccctct ggcgggcgca cagccgccta acgtgattat cggtactatc gtcggcacgg
    11641 cgtttgccat tactactggc gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg
    11701 ctgtcgcagt acagatgggg attaccttcc tgttctcggt gatgtctggc gtaatgtccc
    11761 gctgcgaccg gatggcggag aacgccgata ctcgcggcat tgaacgcgtg aactatctgg
    11821 cattgctggc actcggcact ttctattttc tctgcgcttt cctacctatc tacttcggcg
    11881 cagaacatgc caaaaccatc attgatgtcc tgccgcaacg attaatcgac ggcctcggcg
    11941 tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa
    12001 acgtctacat cccctacttc atcctgggct ttgttgccgc agcctggctc aagttaccgg
    12061 tgttggctat tgctgccgct gcgctggcga tggcgctgat cgacctgctg cgtaaatctc
    12121 ctgaaccgac tcaacctgcg gcacagaaag aggaatttga agatggcatc taatcaaacc
    12181 accctgccga acgtcagcga aaatgaagaa acactgctga ctggcgtcaa tgaaaacgtg
    12241 tatgaagatc agagcattgg cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg
    12301 cgttccatgt tgttacaggc ttctttcaac tacgaacgta tgcaggcttc cggctggctg
    12361 tacggcctgc tgccagcact gaaaaagatc cacactaata aacgcgacct ggcgcgcgct
    12421 atgaaggggc atatgggttt cttcaatacc catccgtttc tggtgacgtt tgttatcggc
    12481 attatccttg cgatggagcg ttctaagcag gacgttaaca gtattcagag caccaaaatt
    12541 gccgtcggtg cgccgctcgg cgggattggc gatgcgatgt tctggctaac gctactgccg
    12601 atttgtggcg ggataggtgc aagcctcgcg ctacaaggct ctattcttgg cgctgtcgtc
    12661 tttattgtgc tgttcaacgt ggtgcacctg gggctgcgtt ttggtctggc gcattatgct
    12721 taccgcatgg gcgtggcggc gattccactc attaaagcaa ataccaaaaa agtcggccat
    12781 gcggcatcta tcgttgggat gacggtaatc ggcgcgctgg tggcaaccta tgttcgttta
    12841 agcaccacgc tggaaatcac cgcgggcgac gcagtggtta agttacaggc tgatgttatc
    12901 gacaaactga tgccagcctt cttaccgctg gtctacaccc tgaccatgtt ctggctggta
    12961 cgccgcggct ggagtccgct gcgcctgatt gcgattaccg tggttctcgg catcgtcggt
    13021 aaattctgcc atttccttta aatacaatga ggtattagat gttaagtatt attttgacag
    13081 ggcatggcgg atttgccagc ggcatggaaa aagcaatgaa gcaaattctc ggtgagcaat
    13141 cgcagtttat cgccatcgat tttccggaaa cctcaagcac cgcgctgctt acatcgcagc
    13201 ttgaagaggc catcgcccaa ctggattgtg aagacggcat tgtttttctg acggatttgc
    13261 tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag
    13321 tgatcaccgg caccaatttg caactgctac tggaaatggt gctggagcgc gaagggttaa
    13381 gcggtgaaga gttccgtgtg caggcgctgg agtgtggaca ccgtgggctg accagtctgg
    13441 tggacgagtt aggccgctgc catgaagaat gtccggtcga ggaaggaata tgacacacgt
    13501 tctgcgcgcc agaaggctgc tgactgaaga gggatggctc gatgaccatc agttgcgtat
    13561 tgctgacggt gtaatcgcag caatcgaacc gattccagtg ggcgtgactg aacgcgatgc
    13621 ggaactgctc tgtcccgctt acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt
    13681 aatggatgac gcgccggatg tgctcgacaa gctggcaatg cacaaagcac gcgaaggtgt
    13741 cggcagttgg ttaccgacca ccgtaaccgc gccgcttaat accattcatg cggcgctgga
    13801 acgtattgct caacgttgcc aacgcggcgg acctggtgcg caagtgctgg ggagttatct
    13861 cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat ccaccggagc tgttccgcga
    13921 gctggaaatt gccgagctgg atcaattgat tgccgtttct cagcatacct tgcgcgtggt
    13981 ggcgctggca ccggaaaaag aaggggcatt gcaggccatc cgccatctta aacagcaaaa
    14041 cgtacgggtg atgctggggc atagcgcggc gacctggcaa caaacccgtg ccgcgtttga
    14101 tgctggtgcc gacggcctgg tgcattgcta taacgggatg acaggtttac atcaccgcga
    14161 accgggaatg gttggcgcgg gattaacaga caagcgcgcc tggctggaac tgatagccga
    14221 tggtcatcat gtacatccgg cggcaatgtc gctctgttgt tgctgtgcga aagagaggat
    14281 cgtactgatc accgacgcga tgcaggcagc cgggatgccg gatggtcact atacgttatg
    14341 tggtgaagaa gtgcagatgc gcggtggcgt tgtccgtacc gcgtccggtg ggctggcggg
    14401 cagtacgctg tctgtcgatg cggcagtgcg caacatggtc gagttgacgg gcgtaacgcc
    14461 tgcggaagcc atccatatgg cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt
    14521 tctgggatcg cttaaaccgg gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca
    14581 tgtgcaacaa atctggattc agggtcaatt agcttcgttt tgatggtttg ttcctttatt
    14641 aggccttcac ttcccccgta aggcctttct ttttctttcg ttttgatctg tgcagcggtg
    14701 tcggatgcga cgctaatgcg tcttatccga cctacggttg gtgagcgtaa ggccggataa
    14761 agtatttacg ccgcatccag caatcccttt tacttccctt accttttctt tcaacgatca
    14821 cagatttcgt tttatttcct ttttctccat tgaactttca gtttcttttc tatagatttt
    14881 aaccaacgaa agaaatcacc aagtgaaatg aaacgaaagg caagcgaaag cgacaacgcc
    14941 gacgtcaagt tcatcagact aaggattgag ttatgccaga aaattacacc cctgctgctg
    15001 ccgcaaccgg tacatggact gaagaagaga tccgccatca gcctcgcgca tggatccgtt
    15061 cactcaccaa cattgacgcg ctacgttccg cgctcaatag cttccttgaa ccgttactgc
    15121 gcaaagagaa tctgcaaatc atcctgaccg gagccggaac atcggcattt atcggtgaca
    15181 tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt cagcgccgta ccgaccaccg
    15241 atctggtcac caatccaatg gactacctga acccagctca tccactactg ttgatctcct
    15301 tcggtcgatc cggcaacagc ccggaaagcg tcgccgccgt ggaactggca aatcaatttg
    15361 taccagaatg ctatcacctg ccgatcacct gcaacgaagc gggcgctctt taccaaaacg
    15421 cgatcaacag cgacaacgcg tttgccctgc tgatgcccgc agaaacgcac gatcgcggct
    15481 tcgcgatgac cagcagcatt accaccatga tggccagctg cctcgcggtt ttcgcacctg
    15541 agatgatcaa cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg atcctgacct
    15601 cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc
    15661 tcggcagcgg tggcttacag agcgcagcac gcgagtcggc gctgaaagtg ctggaactga
    15721 cagcgggtaa actggcggcc ttctatgatt ccccgaccgg attccgtcat ggaccgaaat
    15781 cgctggtcga taacgaaacg ctggtggtgg tgtttgtctc cagccaccct tacacccgtc
    15841 agtatgatct tgatctgctg gcagaactcc gccgcgataa ccaggcaatg cgcgtaatcg
    15901 ccatcgccgc ggaaagcaac gacgttatta ccgccggtcc acatatcatc ctgccgccgt
    15961 cccgtcactt tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc cagacatttg
    16021 cactgatgca gtcgctgcac atgggtaata cgccggatac cccatcagcc agtggcaccg
    16081 ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca ggcataagag gatcgcatta
    16141 tgagcattat ctccaccaaa tatctgttac aggacgccca ggccaatggc tacgcggtgc
    16201 ctgcctttaa cattcataac gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa
    16261 tgcgatcgcc ggtgatcctc gccgggacgc cggggacctt taagcatatc gcgctggaag
    16321 agatctacgc cctgtgtagc gcctattcca tgacctataa catgccactg gcactgcatc
    16381 ttgatcacca cgaatcgctg gatgatattc gccgtaaagt ccacgcaggc gtgcgcagcg
    16441 cgatgatcga cggcagtcat ttcccgtttg ccgagaacgt gaagctggtg aaatcggttg
    16501 tcgacttctg ccactcccag gattgcagcg tggaagcaga actgggccgc ctcggcggtg
    16561 ttgaagatga catgagcgtt gacgccgaaa gtgcattcct gaccgatcca caagaagcta
    16621 aacgctttgt cgaactgact ggcgtcgaca gcctggcggt agcgattggt acggcgcacg
    16681 gcttatacag caaaacgccg aagattgatt tccagcggct ggcggaaatt cgtgaagtgg
    16741 tcgatgttcc tctggtgctg catggtgcca gcgatgtccc ggatgaattt gtccgtcgca
    16801 ctattgaact tggcgtaaca aaagtgaacg ttgccacaga attaaaaata gccttcgctg
    16861 gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc
    16921 gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa aattaatgtc tgtggttcag
    16981 cgaatcgaat ttcagcataa tcattagttt tttatgattt atcccaatgt acttcccgat
    17041 taatatcggg gagtgcctta atggtaaagg agataactaa accttaataa atacatcagt
    17101 acaatatcgc aacaataata tatttaaaaa gattatatta ttcaacttta tggtgaggat
    17161 tacacaatga gcagtccaaa tattctctta acccgtattg ataaccgtct ggttcacggt
    17221 caggttggcg tgacctggac atccaccatc ggtgcgaatc tgttagtagt cgtggatgat
    17281 gttgtcgcta acgatgatat tcaacagaaa ttaatgggta ttaccgcaga aacctacggc
    17341 tttggcattc gtttctttac tatcgaaaaa accattaacg tcatcggcaa agctgcacca
    17401 catcagaaga tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt
    17461 ggtattgacc tgaaagatgt caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa
    17521 atcagcagta aagtttatgt cgatgaccag gatctcacgg acttacgttt tattaaacaa
    17581 cgtggcgtga atgttttcat tcaggacgtc cctggcgatc aaaaagaaca aatccctgac
    17641 taaatctaaa atcgccttaa tattgatttg aggtaataaa aatgcatgaa ataaccctac
    17701 tccagggatt atccctggcg gcgttagttt tctttctggg gattgatttt tggctggaag
    17761 ccttattttt attccgcccg ataatcgttt gtacccttac aggtgctatt ctcggtgata
    17821 ttcagactgg cttaattacc ggtggcctga cagagttggc tttcgccgga ttaacccctg
    17881 caggtggtgt tcagccgccc aaccctatta tggcgggtct gatgaccact gtcattgcat
    17941 ggtctacggg cgttgatgcc aaaacggcaa ttggtcttgg cctgccgttt agtttgttaa
    18001 tgcagtacgt cattctgttc ttctattccg ctttctcatt atttatgacc aaagccgata
    18061 aatgcgcaaa agaggcggat acggcagcgt tttcccgact taactggaca acgatgctca
    18121 tcgtcgcttc agcgtatgcg gtgattgctt tcctctgtac ttacctggca caaggggcga
    18181 tgcaggcgct ggtgaaagcg atgcccgcct agctgaccca cggctttgaa gtggctggcg
    18241 gtattctgcc tgctgttggt tttggcttgc tgctgcgcgt gatgttcaaa gcacaatata
    18301 tcccttacct gatcgccggt ttcctgtttg tttgctacat ccaggtcagc aacctgttgc
    18361 cggttgccgt actgggcgca ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc
    18421 agcaagcgca accgcagccc gttgccagta aaaatgaaga agaggactac agcaatggga
    18481 tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc gttcgtcact gctacaagca
    18541 agctttaact acgaaaggat gcaggcgggc ggttttacct gggcgatgtt gccgatcctg
    18601 aaaaagattt ataaggacga caaaccgggc ctaagcgcgg cgatgaaaga taacctcgaa
    18661 tttattaata cccaccctaa tctggtcgga ttcctgatgg ggttattaat ttcgatggaa
    18721 gaaaaaggag aaaaccgcga caccattaaa ggcctcaaag tggcgctgtt tggcccaatc
    18781 gccgggattg gcgatgcgat tttctggttt accttgttgc caattatggc ggggatttgc
    18841 tcatcgtttg ccagtcaggg gaacctgttg ggaccgattt tgtttttcgc cgtttacctg
    18901 cttatctttt tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg cgtgaaggct
    18961 atcgataaag tgcgagagaa ctcgcagatg attgcccgtt cggcaaccat cctcgggatc
    19021 acggtaatcg gcgggctgat cgcttcgtat gtgcatatta acgtggtgac gtcgtttgcc
    19081 atcgacagta cccacagcgt cgcactgcaa caggatttct tcgataaagt cttcccgaat
    19141 attttaccga tggcctacac cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat
    19201 ccggtgctgt taatcggcgt gacgtttgtt ctttctattg tttgttctgc attcggcatt
    19261 ttgtaaatgg aacgaggcac cgcgtctggc ggtgcctctt tacttaagga atttcatcct
    19321 gtgcaaaccc ttcaacaaat tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat
    19381 ttattggccg tgatccgtcg caaaccggat gccgtgattt gcctggcgac cggagccacg
    19441 ccattactga cgtatcatta tctggtagaa aaaatccacc agcagcaggt tgatgtcagc
    19501 cagctcacct tcgtgaagct cgacgaatgg gtggatctgc cattaacgat gcctggtacc
    19561 tgcgaaactt tccttcaaca gcatatcgtg cagccgctgg ggctacgtga agaccagctc
    19621 atcagctttc actccgaaga gataaatgag acagagtgcg aacgggtaac gaacctgatt
    19681 gcgcgcaaag gcggtctgga cttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg
    19741 ctgaacgaac cgggagaaag cctgcaaccg gcctgccata tcagtcaact tgatgccaga
    19801 acacagcaac atgagatgtt aaaaaccgcg ggccgtcccg tgactcgtgg gatcacctta
    19861 ggcctgaagg atattctcaa tacccgcgaa gttttgctac tggtgactgg cgaaggaaag
    19921 caggatgcga cagaacgttt tctcacggct aaagtctcta ccgctatccc ggcttcgttt
    19981 ttatggctgc ataaccattt cacatgcgtg attgatgaaa tgtgtcgtcg ttaactcgcc
    20041 tggtaaaggc gttgtagcgc tattcctgct gcaacgcctg taatcactct ccctgctgct
    20101 ccagtgcata cttatacagc gcatttttct tcacgccgtg aatttctgct gccagcgccg
    20161 ccgctttttt cagtggcagt tctgcctgca acagcgccag cgtgcgcagg gcatcggcgg
    20221 gtaagtcttc ttcctgtgct ttatggcctt cgacaatcag taccatttcg cctttgcgac
    20281 ggttttcatc ttctttgacc cacgccagca gctcgccaac gggcgcgccg tgaatggttt
    20341 cccaggtttt ggtcagctca cgcgccagaa cgacgtagcg ggattcgcct aataccgcaa
    20401 cgatatcttc caggctatct aacagacggt gggtagattc ataaaaaatc agcgtgcgcg
    20461 gctccgcttc aatggctttt agcgcatcac ggcggccttt tgatttggca ggtaaaaagc
    20521 cttcataaca aaaacgatcg gatggtaaac ccgcggcgct taacgcagtg atagcagcac
    20581 acggcccggg tagcggcacc acgcgaatcc ccgcttcacg gcaggtacgc accagatggt
    20641 agccaggatc gttaattagc ggcgttccgg catcggaaac cagcgcaatg ttttgcccct
    20701 cttgcagctt cgccagcagc gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa
    20761 acaaccgggc attaatccca aaatgttgca gcaataaacc ggtgtgacgc gtatcctcgg
    20821 cggcaatcag atcaacggcc tgtaatacct ctaatgcacg ctgggtgata tcagccagat
    20881 tgccgattgg cgtcggtaca atgtaaagct ggccctgaga attatccgcc gattggtgtt
    20941 gtttcattgt gtcgtccgta ttgccgattt aatattgagc attgcgtaaa aaaaatatca
    21001 ctggatacat tatggtaccc tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg
    21061 ttgttctggc agccctgatt ttcgccggtt gtggcaccca tactcccgat cagtccactg
    21121 cttatatgca gggcacggcg caggctgatt ctgcctttta cctgcaacag atgcagcaaa
    21181 gttctgatga taccaggatc aactggcaat tactcgccat tcgtgcactg gtgaaagaag
    21241 gtaaaaccgg acaggcggtg gagttgttta accaactacc gcaagaactg aacgattctc
    21301 agcgtcgcga gaaaacactg ctggcggcag agattaaact ggcgcagaaa gattttgctg
    21361 gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt agaacaaaac cagcaagccc
    21421 gttactggca ggcaaaaatc gatgccagcc aggggcgtcc ttccattgat ttactgcgcg
    21481 cgttaattgc tcaggaaccg ctgctcggtg cgaaagaaaa gcagcagaat attgacgcca
    21541 catggcaggc gctctcctcc atgactcagg agcaggcgaa tacgctggtg atcaacgccg
    21601 acgaaaatat tctgcaaggc tggctggatt tgcagcgcgt ctggtttgat aaccgtaacg
    21661 atcctgacat gatgaaagct gggatcgccg actggcagaa acgttatccg aacaacccgg
    21721 gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc gtttaaacca gcatcgacca
    21781 acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc ggtatttggt cgcactattc
    21841 agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc agtggcggcc caggtagctg
    21901 ccgcacctgc cgcagacgta gcagaacaat ctcagccgca aaccgtggat gacgttgcca
    21961 gcccggcaca agcctcggtt agcgatctga ccggtgaaca gcctgcggcc cagccggtgc
    22021 ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc acccgcaaat ccatccgcag
    22081 agctgaaaat ctacgatacc tcatcacaac cacttagcca gatcttaagc caggttcagc
    22141 aggatggcgc gagtattgtg gtcggtccgt tgctgaaaaa taacgttgaa gagttgctga
    22201 agagcaacac tccgctgaac gtgctggcac tgaaccagcc ggagaacatc gaaaaccgcg
    22261 tcaatatttg ttacttcgcg ctttcaccgg aagacgaagc gcgcgatgca gcgcgtcata
    22321 ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc acgcagttca ttgggcgatc
    22381 gcgtagccaa tgcgtttgcg caagagtggc agaaactggg cggcggcccc gttctgcaac
    22441 aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa cggcggttcg ggtatcgctt
    22501 taacgggtag cccgattact cccagagaaa caaccgactc cggcatgacg accaacaatc
    22561 caacgctgca aaccacgcca accgatgatc agttcaccaa taatggcagt cgtgtcgatg
    22621 cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat caaaccaatg atcgccatgc
    22681 gtaacggtag ccagagcggt gcaacgctgt acgccagctc ccgcagtgcg caagggaccg
    22741 ctggcccgga tttccgcctg gagatggaag gcttgcagta cagcgaaatc ccgatgctgg
    22801 caggcggtaa tctgccgtta atgcagcagg cgctcagcgc ggtgaacaac gattattcgc
    22861 tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgtt ggcaaatcat ttctcacaaa
    22921 tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg aagcctgacg gcaaacccgg
    22981 attgcgtgat taacaggaag ttatcatggc tacagtacca acaaggtcag gtagtccccg
    23041 ccagttaacc accaaacaga ccggcgatgc gtgggaagca caagcgcgtc gctggctgga
    23101 aggcaaagga ctacggttta tcgccgctaa cgtgaacgag cgtggcggcg agatcgatct
    23161 gataatgcgt gaaggccgga ccaccatttt tatcgaggtg cgttaccgcc gctctgcgct
    23221 ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa cacaaattat tacagactgc
    23281 ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact gtggattgcc ggttcgatgt
    23341 ggtagccttc accgggaatg aggttgagtg gattaaggat gcctttaatg accactcata
    23401 attaaggttt aaggattagc gtgcaagaaa gaattaaagc ttgcttcact gaaagcattc
    23461 aaactcaaat tgcggcggca gaggcgcttc cggatgccat ctcccgtgca gccatgacgc
    23521 tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg tggtaatgga acttccgctg
    23581 ccaatgcaca gcattttgct gccagcatga tcaaccgttt cgaaacggag cggcccagct
    23641 tacctgccat tgcactaaat actgataatg ttgtcttaac ggcaattgcc aacgatcgct
    23701 tacatgatga agtgtatgca aaacaggtgc gggcgctggg tcatgcggga gatgtattgt
    23761 tagccatttc cacccgtggc aacagccgcg atattgtaaa agcggtagaa gctgccgtta
    23821 cgcgtgatat gaccattgtg gcattaaccg gctatgacgg cggtgaactt gcaggtttgt
    23881 tagggccaca ggatgtggag atccgcattc cttcgcatcg tagtgctcgc attcaggaaa
    23941 tgcatatgct gacggtaaat tgcctgtgcg atctgatcga taacacgctt ttccctcacc
    24001 aggatgttta aggagaatac atgaaggcat tatcgccaat cgcagtcctt atttccgcgc
    24061 tgctgttgca aggttgtgtt gccgctgccg tagtgggtac tgctgctgtg ggtaccaaag
    24121 ccgcaactga cccacgcagt gtcggcaccc aggtagacga tggtaccctg gaagtgcgcg
    24181 tgaacagcgc attgtcgaaa gacgaacaaa ttaagaaaga agcgcgcatt aatgtaacgg
    24241 cctatcaggg caaagtgctg ctggttgggc agtcaccaaa cgctgaactt tcggctcgcg
    24301 ccaaacagat tgctatgggc gtagacggtg ccaacgaagt gtataacgag attcgtcagg
    24361 gccagccgat tggtctgggc gaagcgtcta acgatacgtg gatcaccacc aaagtacgtt
    24421 cgcagctgtt aaccagcgac ctggtgaaat cgtccaacgt gaaagtgacc accgaaaacg
    24481 gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc gaaagcggcg gcagatattg
    24541 ccagccgggt aagcggcgtg aagcgggtaa ctacggcgtt tacgtttatt aaatagcagc
    24601 ccttgtaatg cctgatgcga cgcttgcagc gtcttatcag gcctacaacg atgcaaaccg
    24661 taggtcggat aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc aaaccaacag
    24721 cgccagcccg ccgacaatca caccagacac tgccaccatc gcccccgtca gccataaggc
    24781 tttcgccggg aacgctttgc gcagcataat cagtgacggc aaactcaccg ccgggagcgt
    24841 catcaacaat gccagcgccg gagcagttcc catacctgcc agcatcatcg tttgtacaat
    24901 cggaatttct gctgccgtgg gaatgacaaa caagcatcct gccaccgcca tcgccaccac
    24961 ccacatcagg ctgttatcga cagcaccatc ggcatggggg aataaccaga cgcgagcggc
    25021 acccaacacc agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa gcgtccatag
    25081 cgccctgccc cagcggctaa aaaaaccgcc ttgtgattcc ggtatgtcaa tttcgaccgg
    25141 tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac
    25201 catcaccagc cccgccacca gacgaatcgc cgcaaaaccc cagccgagga cgaaccccat
    25261 aaacaccagc gtcgccgggt ttaacaacgg attccccatc cagaatgcca gcgcaccgcc
    25321 catcgacacc tgttggcgac gcatccccgc cgcgaccgga gccgcacagc aggtacacat
    25381 catacccggc agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag
    25441 cgtacgcagc aaccagtcac gcgggatcag cacctgaatc aacgaaccga ggatcacccc
    25501 cagcaccgcc gctttccata ccgcgaggaa atagaccatc gcgtaatcca acgccgcctg
    25561 ccacggatta gcatccgcct gcgcaaggat agatttaccg atactgtgag tttcggcggc
    25621 agtaaaggct ttaccgtagt aaggttgcca tttcacatac cagagaccgg caatgacaac
    25681 gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt gccgcctgag atgaagactg
    25741 accagtcata gcattcccca ggaataagtt atgataattg agcgcgtgaa tattacgctc
    25801 actatcaatt cttgggaata attattcagc tcttttgcgt aattctgaag agcttaaaat
    25861 cgtcacacct tcatgctcag gccgcatcgc ctctgccagc atgacccgcg ccacgtctct
    25921 cgcatcaatg gatttccagt taccgggtaa caaacggaac agcggcgcaa aaagcgtttc
    25981 gttcatccgc tgtttgctac gatcgcccag taacatcgac gggcgagcaa tggtcaattt
    26041 cggccagttc tgggcgatta atgcttcttc catctcccct ttgacgcggt tatagaaaaa
    26101 cggcgagtgg gcattagcac ccatagcact gaccaccagc atatgctgcg caccgagccg
    26161 ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg gcatgaataa acgcctcttt
    26221 actccccgct tctcgccgcg tggtgccgag acaacaaaac acaatgtcga tgggatcggt
    26281 gacctgcgcc agcgcgtcgg tcagttgcgg atcatgggga ttgaacactc ctggcatatc
    26341 tcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt taatcaacat
    26401 ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta atcagtacct gactcatctt
    26461 cgctccttta cagaattgtc tgccttgcgc tccacggctc atgcaccagg cttaatagat
    26521 catgaggtaa ttatccccga ttgtggaaaa ttcgcctcat ccaatgcaac aacgcggagg
    26581 aagcatgagt aagaaaatcg ccgttttaat cactgatgaa tttgaggatt cagaatttac
    26641 ttcacccgca gacgagttcc gtaaagccgg acacgaagtg attaccattg aaaaacaagc
    26701 gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg gccatcgata aatccatcga
    26761 tgaagtgacg cccgcagagt tcgatgccct gctgctaccg ggcggtcatt caccagatta
    26821 tctgcgtggt gacaaccgtt ttgtcacctt cacacgcgat tttgtgaaca gcggcaaacc
    26881 tgttttcgcc atctgtcacg gtccgcagct gctgatcagc gccgatgtga ttcgggggcg
    26941 caaactgact gccgttaaac caatcattat tgatgttaaa aacgcgggcg cggaatttta
    27001 cgatcaggaa gtggtggtcg ataaagatca gttggttacc agccggacac cggacgatct
    27061 gcccgcgttt aaccgcgagg cgttacgcct gctcggtgcc tgagttacgt agccaaatca
    27121 tttttttacc aaagcccagc gtgttgtcgg tgaatttgat ttcatcgagc cggatttccc
    27181 ataccggggc cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc ttgcgtgcga
    27241 ggtcgctttc ctcgccttcc agtctgcgga tctcaccttt aaactgcacg ccgcgaatta
    27301 acgctaccgt tttcggctga ccgtttaccg ttccggcaat tgccgcctgc ggcccgctca
    27361 tctgcgcatg acgcgttttt tcttccgtca aaatgtagaa ggcgactttc tgcgcatcaa
    27421 aaagataaaa ggcattggcg caccacagtt ccccttcctg ctgcacacac caggtgacaa
    27481 catgttgttt tgccagccaa cggctgatgg cggtgagtgt ttccattgct gttctctttt
    27541 atactgtggg cctgacctta acatgcgacg cagacgatga caccctggtt tctttacttg
    27601 atccgtaccg ccgacaataa gctttatacc gggatcacca cggatgtcga acgccgctat
    27661 cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg ggaagggaga actaacgctg
    27721 gcgttttccg caccagtggg cgatcgttcg ctggcgttac gggcagaata tcgcgttaag
    27781 caactgacca aacggcaaaa agagcgtctg gtagcagaat gtgcagtgtt tgcagaactg
    27841 ttgaacagcc tgcaaacccc ggagattaaa agcgattgaa gtgctcgtga tactcaacca
    27901 gaccagttac accgttcagc gcgtcatccg ctaaacgatg tacctggaag gcactttcag
    27961 tgcccggcca gcggcaacgc agatcgtgat gtgccgccag ttcaaagccg aaacggctgt
    28021 acagcgccgg atcgcccagc gtcaccactg cggcatagcc gaactcatta agcgaatcga
    28081 gtccttcata gaccagttgg cgtgccagcc cctgtccacg gtatttttca tctaccgcca
    28141 acggtgccat gccgacccat tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca
    28201 catagccaat gacctgacct tcgtcatctg tcgccaccag ccccagcgtc agaaagccat
    28261 cttcacgcag atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca
    28321 gggcatcaat acccggcgca tcaatgggaa tttctactcg aattagcatg gttcacctac
    28381 cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg
    28441 cagcagcatc atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt
    28501 tttcacatac agccccagct ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag
    28561 cgtatccgga tcttgtttac gcgccgcaat catcagcaga tcgctggcgt cggcactaaa
    28621 actcacatca gcttgcgcgt tctggctaac gaccagtttg ccattcacca ccgaggtaaa
    28681 ccattgcagg tcaatatcac gcacatgaat acttaaccag cgaccttcaa gaaactccag
    28741 ctcgccatca tccagcgcct gacggaattg ccagcttaag acctgctcaa gaacctggcg
    28801 ttttagcgca aatggcgtca gttttaccgg tacactcaac agagatggcc ccaaatgcac
    28861 aatacgggaa cgcagtttat ccaacacgag ttttactccc tgtttcaaca atcatcctat
    28921 tttgccatat cagaaaaata acatagcggt ataaatcaac aattccatat gaaattgctg
    28981 ctaccaccaa tacaacttta actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa
    29041 aatcccagtt cgttaacatt tttgcgtttt gataacgcaa ccttcaggaa aaattatgga
    29101 gctgctctgc cctgccggaa atctcccggc gcttaaggcg gccatcgaaa acggcgcaga
    29161 tgctgtttat atcgggctaa aagatgatac caatgcccgt cacttcgccg gccttaactt
    29221 taccgagaaa aaattgcagg aagcggtgag ttttgtccat caacatcgcc gcaaacttca
    29281 catcgcgatt aacacttttg cgcatccgga cggttacgcc cgttggcagc gcgccgtgga
    29341 tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca tgctggagta
    29401 cgccgccgag cgttatccgc atattgagcg ccacgtatcg gtgcaggctt cggcgaccaa
    29461 tgaagaggcg attaactttt atcatcgcca ttttgacgtt gcccgcgtgg tgctgccgcg
    29521 cgtgttgtcg attcatcagg tgaaacaact ggcacgggtc acacctgtac cactggaagt
    29581 ctttgctttc ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt cgtcgtattt
    29641 gacgggtgaa tcgcccaaca ccgtgggtgc gtgttctccg gcccgtttcg tgcgctggca
    29701 acaaacgccg caggggctgg aatcccgcct gaacgaagtg ctgatcgacc gttatcagga
    29761 cggcgaaaac gcaggttatc cgacgctgtg taaagggcgt tatctggtgg acggcgagcg
    29821 ctatcacgcg ctggaagaac caaccagtct caataccctt gagctgctgc cggagttaat
    29881 ggcggcgaat atcgcttcgg tgaaaattga aggccgccag cgtagcccgg cgtatgtcag
    29941 ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt
    30001 cgtgccgcaa agcgcgtgga tggagacgct cgggtcgatg tccgaaggca cgcaaactac
    30061 ccttggcgcg tatcaccgta aatggcagtg agaaaagcaa tgaaatattc cttagggccg
    30121 gtgctatggt actggccaaa agagacgctg gaagaatttt atcagcaggc cgccgccagc
    30181 agcgcagacg tgatttatct tggtgaagcg gtctgtagca aacgccgggc aaccaaagtt
    30241 ggcgactggc tggagatggc aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc
    30301 acgctggcgc tggtgcaggc atcctccgaa ctgggcgaac tgaaacgcta tgttgagaac
    30361 ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa
    30421 ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg cggtgacgct gaaaatattg
    30481 ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga ctggctggtg
    30541 aatctgctta atcagtgcga tgagttgggc attcgcaacc agtttgaagt ggaagtcctg
    30601 agctacggtc atctacctct ggcctactcc gcccgctgct ttaccgcgcg ttcggaagac
    30661 cgcccgaaag atgagtgtga aacctgctgc attaaatatc cgaacgggcg caacgtactg
    30721 tcgcaggaaa accaacaagt gtttgtactc aacggcattc agaccatgag cggctacgtt
    30781 tacaacctcg gtaacgagct ggcatccatg caggggctgg tcgatgtggt ccgcctgtca
    30841 ccgcagggta ccgacacttt cgcgatgctc gacgccttcc gcgctaatga aaatggcgct
    30901 gcgccactcc cgttgacggc aaacagcgac tgtaacggct actggcggcg gctggccggg
    30961 ctggagctgc aagcgtaagt atatagctca ctttgttaac aactttaact actctttaat
    31021 gcagtattaa agactaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt
    31081 tttcgctact cgatctggcc cccatccccg aaggttcttc agcgcgggaa gcgttctccc
    31141 actctctcga cctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag
    31201 aacaccacaa tatgaccggc attgccagtg ctgcaacgtc ggtgttaatt ggctatctgg
    31261 cggcgaatac caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac
    31321 cattggtgat cgccgaacag ttcggcacgc tcaatacgct ctatccgggg cgaatcgatt
    31381 tggggctggg tcgtgcaccg ggtagcgatc agcgaaccat gatggcgctg cgtcgtcata
    31441 tgagcggcga tattgataat ttcccccgcg atgtcgcgga actggtggac tggtttgacg
    31501 cccgcgatcc caatccgcat gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt
    31561 ggttgttagg ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgtttg
    31621 cgtttgcctc acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca
    31681 acttcaaacc gtcggcacgg ttggagaaac cgtatgcgat ggtgtgcatc aatattatcg
    31741 ccgccgacag caaccgcgat gccgaattcc tgtttacctc aatgcagcaa gcctttgtga
    31801 agctgcgccg cggcgaaacc gggcaactgc cgccaccgat tcaaaatatg gatcagttct
    31861 ggtcgccgtc cgaacagtat ggtgtgcagc aggcgctgag tatgtcgctg gtgggcgata
    31921 aagcgaaagt gcgtcatggc ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta
    31981 tggttaacgg gcagattttc gaccaccagg cacgcctgca ttcatttgag ctggcgatgg
    32041 atgtgaagga cgagttgttg gggtagtgtg ttttaacgta ggaagcctta tccgagatgg
    32101 caacgctgtc ctacgtaggc ctgataagcg aagcgcatca ggcattgtgt aggcagcaga
    32161 aatgtcggat aaggcaccgc tgattactga tacaccggca gcacattaaa gctcgataag
    32221 atatggacca gtgcgttgcc aacgccaaat accagaatca gcataatcat cggcttgccg
    32281 ccccagacac ggaatttcgg gctaccaaag cgtttacgag atgcgcgggc caacagcgcc
    32341 ggaacaattg ccgcccagat ggtagccgct aagccagcat aaccaatggc gtacaggaat
    32401 ccgttaggaa acagcaggcc acccacaaca ggcggggcaa aggtcagcaa cgctgttttc
    32461 aaacggccca tagccgagtc gtcaaaacca aacagatctg ccagatagtc aaacaaaccc
    32521 agcgttacgc caaggaacga actcgctacc gcaaagtttg agaacacgac cagtagaaga
    32581 tccagactac ggctgttcag tacgccgctt aacgcctgta ccagcacatc aatattaccg
    32641 cccttctcgg cgatgccgat aaactccgga cgcgggatgt tacccatcgt cgccagcaac
    32701 cagatggtat acagcgccag cgccatcagc gtaccgtaca ccaggcattt cacgatggtt
    32761 ttcggatctt tgccgtaata cttcatcagg ctcggtacgt taccgtgata accaaacgat
    32821 gccagacaga acggcagcgt catcaacaga tacggtgcat aagacgcatt gctttcggcg
    32881 acgttgaaca atgtcgcagg ctgcacatgc cccagcaggc taccgaaggt gaggaagaag
    32941 gtaatgactt tcgcccccag cacaatcgct gtcatgcgac tgacggcttt ggtgctcaac
    33001 cacaccacaa acgccaccaa cagtgcaaaa ccaaaacccg ctgcccgtgc cgggacgttt
    33061 agcgacatct ctgcgaaggt gtgatgcaga atcgaaccac tggcagaaat ataggcatag
    33121 gtcaggatgt agagcacaaa ggcaatagaa atgccgttga ccacgttcca gcctttgccg
    33181 agcaggtctt tggtgatggt gtcaaagctc gaaccgattc ggtaattcag gttagcttcc
    33241 agaatcatca agccggaatg cagcatacag aaccaggtga aaatcagcgc cgccattgac
    33301 cagaagaacc acgccccgga catgaccact ggcagagaaa acatccctgc gccaataatg
    33361 gtgccgccga taatcaccac gccgccaagc agcgacggtg acgtttgggt ggtggttagt
    33421 gttgccatga gggcttctct ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac
    33481 tgtaccagta catgagtaca aaagacagaa aaaaagcccc gatggttaaa atcggggctg
    33541 tatatattat tttacagact gtgttcgctg ttcagcgatg attacgcatc accaccgaaa
    33601 cgacgacgac cggtagagtc atcacgacgc ggtgcgcggc cttcacgacg ttcgccgcta
    33661 aaacgacgcc catcaccacg acccccttca cggcgttcac cgctgaagtt acgaccgcct
    33721 tcacgacgtt cgccaccgaa accacgacca ccgccacgac gctcaccgcc agtatgcggc
    33781 tgtgcatcgc ccagtaactg catgttcatc ggcttgttga gaatgcgagt acgcgtaaag
    33841 tgttgcagca cttcacccgg catacctttc ggcagttcga tggtggagtg agaagcaaac
    33901 agcttgatgt taccgatgta acggctgctg atgtcgcctt cgttagcaat cgcgccaacg
    33961 atatgacgaa cttcaacacc atcatcgcgg cccacttcaa tacggtacaa ctgcatatcg
    34021 ccaacatcac gacgttcgcg acgcggacga tcttcacggt cgccacgcgg gccacggtcg
    34081 ttacgatcgc gcggaccacg gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc
    34141 gcatctggcg gaacaatcag agtacgttca ccctgtgcca ttttcagcag tgcggcggtc
    34201 agagtttcga gatccagctc ttcaccttct gccgtcggct gaattttgct cagcagtgca
    34261 cggtattgat ccagatcgct gctttccagc tgctgctgta ctttagcggc gaatttttcc
    34321 agacgacgtt tgcccagcag ttctgcgttc ggcagttcaa cttccggaat ggtcagcttc
    34381 atggtgcgtt caatgttacg cagcagacga cgctcacggt tctcaacgaa cagcagcgca
    34441 cggccagcac gacccgcacg accggtacga ccgatacggt gaacgtaaga ctcagaatcc
    34501 atcgggatat cgtagttaac caccaggctg atacgctcaa cgtccaggcc acgggccgca
    34561 acgtcggtcg caatcaggat gtccagacga ccatctttca gtcgttccag tgtctgctca
    34621 cgcagcgcct ggttcatgtc accgttcagc gcggcgctgt tgtagccgtt acgctcaaga
    34681 gcttctgcca cttccagagt cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa
    34741 tcttccgctt ccaggaaacg taccagtgct tcgtttttac gcatacccca gacagtccag
    34801 tagctctggc tgatgtcagg acgggtagtc acgctggact gaatgcgcac ttcctgcggc
    34861 tctttcataa agcggcgggt aatgcgacga atcgcttccg gcatggttgc agagaacaga
    34921 gcggtctgat gaccttccgg gatctgcgcc ataatggttt caacgtcttc gatgaagccc
    34981 atgcgcagca tttcgtcagc ttcatccaga accagaccgc tcagtttaga gaggtccaga
    35041 gtgccacgtt tcaggtggtc cagcagacgg cccggagtac cgacaacgat ctgcggcccc
    35101 tgacgcaggg cgcgtaattg cacgtcataa cgttggccgc cgtacagggc aaccacgttt
    35161 acgccgcgca tgtgtttaga gaaatccgtc attgcttcag caacctgtac cgccagttcg
    35221 cgggtcggtg ccagcaccag aatctgtggt gctttcagct caggatcaag attctgcagc
    35281 agaggtagag agaatgctgc agtttttccg ctccccgtct gggccatacc cagaacgtcg
    35341 cggccattca gcagatgtgg aatacactct gcctgaattg gagatggttt ttcgtaaccc
    35401 agatcgttaa gggcttcaag gataggagcc ttcaggccca gatctgcaaa agtggtttcg
    35461 aattcagcca tgtagtacgt gtgcctcaaa attaatggcg gccagtctac ataactcatc
    35521 atgaaattga tcagcaattt tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa
    35581 cgaacaacaa cgccctcacc cgttaaggtg atggcaatca aaaaagatta cgggctgatg
    35641 tgtacgtcag ctattgctgg tccgattctg ccaggtcatc ttggtcctgg cccaggagcg
    35701 ataattccaa caatgcgtat cggtgctcaa caaagttatg aacgttgttg gcaaccgcca
    35761 gtttgaacag tgccgtggcg ctgtccaaat cccccagact taggtagtac ttacctaaat
    35821 agaagttggt ttcactgaga tgctcagcga gcgaggtgtt atccgttgcg tccgccttga
    35881 gcctttccat taacgtttgt tcgctaatgt tgcccaggta gaactcgaca atgttccatc
    35941 cccactgttc cttatccgat ttttcgaagt gctgtttcaa cacttcttta gcctgcttct
    36001 catcgagctt ctgctcggcg agataaagcc acagactacg gaaaggatca ttgggatcgt
    36061 cttgataaca cgccagcaga tcatcttgcg ctaacttgtc acgaccgccg taatataatg
    36121 cgatcccgcg attcaagtgc gcgtagttgt aagttggatc aagctcaagt acagaatcaa
    36181 acgcttcata ggcagcatca aaattgcctg cctgcgttaa atatatgcct aagtaattga
    36241 atacttcagg catatccggt cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg
    36301 ccctcagacc gagactatca tacaacactc cgcgctcata taaaagctgt gcgcgttcgt
    36361 catcggttaa agcccgactg gcaaggatct gttccatacg tgccagaatc acttcctgct
    36421 gtaaagtcgg ttgcaatggt accgcgagga cttcactttt acgccaggaa gtattactgc
    36481 atcctgcaag cgtaagtgct gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc
    36541 actcccgaag accacggttg aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc
    36601 ccggttaaaa gccccccgcc gcagcggagg gcaaatggca accttactcg ccctgttcag
    36661 cagccggagc ttccggtgct gcagcaggtt gagactgctc agtcgcttct ttaatgctca
    36721 gacggatacg gccctggcga tcaacttcca gcactttcac cggtacttcc tgacccatct
    36781 gcaggtaatc ggtcactttc tcaacgcgtt tatcagcgat ttgagagatg tggaccagac
    36841 cttctttacc gccaccaatg gcaacaaatg cgccaaagtc aacgatacgg gtcactttac
    36901 cattgtagac gcggcccact tcgatttctg cagtgatctc ttcgatacga cgaatcgcgt
    36961 gtttcgcttt ctcgccgccg gtcgctgcga tcttcacagt accgtcatct tcaatttcga
    37021 tggtggtgcc ggtttcttcg gtcagagcac ggattacaga accgccttta ccgataacat
    37081 ctttgatctt gtccgggttg atcttgatgg tatggatacg cggtgcgaac tcagagatat
    37141 cgccacgcgg cgcgttgatc gcctgttcca ttacccccag gatatgcaga cgcgcacctt
    37201 tagcctggtt cagtgcaacc tgcatgatct ctttggtgat accttcaatt ttgatatcca
    37261 tctgcaatgc agatataccg tcacgggaac ccgcaacttt gaagtccata tcgcccaggt
    37321 gatcttcgtc gcccaaaatg tcagacagta caacgtagtt gtcgccttct ttcaccagac
    37381 ccattgcgat acccgcaacg gcagctttga tcggcacacc tgcgtccatc agcgccagag
    37441 acgcgccgca cacggaagcc atagaagagg aaccgttgga ttcggtaatt tcagacacaa
    37501 cacgtacggt gtacgggaat ttgtccatat ccggcattac tgccagcacg ccgcgcttcg
    37561 ccagacgacc gtgaccaatt tcacgacgct tcggagaacc gaccatgccg gtttcgccta
    37621 cggagtacgg agggaagttg tagtgaaaca ggaaggtatc ggtacgttcg cccatcagtt
    37681 catcaagaac ctgcgcgtca cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt
    37741 caccacgggt gaacagcgca gaaccgtgag tacgcggcag cacaccagta cgcacatcca
    37801 gaccacggat catatctttt tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc
    37861 tacgaacaac atttttctcg atagcgtgca gaatttcacc cagttcgttt tcgtccaggg
    37921 tttcgtcttc agcaagcagt gtcgcgatgg tttcagattt gatgacatca acctgcgcat
    37981 aacgctcttg tttgtcggtg atgcggtaag catcgctcag acgagcttca gccagcgcag
    38041 caacgcgcgc gttcagcgct tcgtttaccg gctccggctg ccagtcccaa cgcggtttac
    38101 cggcttcttt caccagttca ttgatgttct gaataacaac ctgctgttgt tcatgaccga
    38161 acactactgc gcccagcatc tgatcttcgc tcagcagttc agcttcagat tcaaccatca
    38221 gcacggcagc ttctgtaccg gcaacaacca agtccagttt gctctctttc agttcatcct
    38281 gagtcgggtt cagtacgtac tggtcattga tgtaacctac acgggcagca ccaatcgggc
    38341 cattgaacgg aataccagac agagacagtg ctgcggaagc accaatcatc gcgacgatat
    38401 ccgggttaac ctgcgggtta acagaaacca cggtggcgat aacctgaact tcgttgacga
    38461 agccttccgg gaacagcggg cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc
    38521 cttcgcttgg gcggccttca cgacggaaga agctacccgg gatacgacca gcagcgtagg
    38581 tacgctcctg atagttaacg gtcagcggga agaagtcctg acctggtttg gcttttttct
    38641 gaccaacaac ggtaacaaat accgcggtgt catccatgct aaccataaca gcggcagtag
    38701 cctgacgagc catcatgccg gtttccagag tcacggtgtg ttggccgtac tggaatttac
    38761 gaacgatcgg attaagcaat gtaatatcct ttctctttct tggacagtac cttacggtac
    38821 tggtgttaat acccgatctt ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc
    38881 tatgtgggta aagcctctca ttagccgcgc gaacctctgc aacggaagat cattcatagc
    38941 aacaatacat tagtttccag tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg
    39001 cccccttttc tgaaactcgc aagaattagc gacgcagacc caggcgctcg atgagctggg
    39061 tgtaacgtgc tacgtcttta cgtttcaggt agtcgagcag tttacgacgc tgagaaacca
    39121 tgcgcagcag accacgacgg ctgtggtgat cttttttgtg ctctgcaaag tggccctgca
    39181 ggtggttgat ctgtgcagtc agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg
    39241 cgtcacgacc aaactcagaa acgattttag ctgttgcttc agtacttaga gacattttaa
    39301 aactccaaag tatatagaat gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg
    39361 ttacgcaaag tgttaaacaa tttacgcgac gttaagcggc agtattctac tcgtagcgac
    39421 ctgttatcgc aagacggtta acattacgcc gggtattcaa ccaccaggcg acgaggcgca
    39481 acgcggcctt catcgtcaat ttcgcccata ccgataaact tgccgttctc accctctgta
    39541 acgcgaacca gtccttccag cggcgcaccg gatgtacgaa ctgggttacc atttttgaag
    39601 taaacagaag acgttaacgg aagattcacc accgggtagt ccgaagctgg actgtccatt
    39661 ggcatcagta atggatcaag taactccgca gccggaatat cctgctgttc ggcttgctca
    39721 accagttcac gcagatgttc cagggtcacc atccgttcaa ccggatattt acttaccgcc
    39781 agacgacgca agtaaataac atgcgcgcca caaccgagtt tttcgccgag atcgtcaatg
    39841 atagtgcgga tataagtgcc ttttgagcag tgaatttcca gctccagctc attgccttca
    39901 tggcgaataa acagcaattc ataaacggta atcgggcgcg cttcacgtgg aacttcaatg
    39961 ccctgacgcg cgtattcgta cagttttttg ccctgatatt tcagtgctga atacatcgaa
    40021 gggatctgtt cgatatcgcc acggaaggta tccagcgccg ccgccagttg ctctgcacta
    40081 aaggttaccg gacgttcttc aacgatttgt ccgtcggcat cagaagtatc ggtacgctgc
    40141 ccaagacgcg caatgacccg atagcgtttg tcggagtcca gcagatactg ggaaaacttc
    40201 gtcgcttccc cgaggcaaat tggcaacatg ccggtcgcca gcgggtccag cgcaccggta
    40261 tgcccggcac ggttggcgtt ataaatccgt ttcacttttt gcagcgcatc gttgctggac
    40321 atcccctgcg gtttatccag caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga
    40381 cgactcatta gtcctccttg ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga
    40441 ccacgctggt caccaggttt gacatgcgca tcccttcaac cagagagttg tcgtagaaga
    40501 aggtcagttc cggcacgata cgcaggcgca tcgctttccc cagcaggctg cggatgaaac
    40561 cagaagcttc ctgcaacgct ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga
    40621 ggaacgtcac atatactttg gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg
    40681 tggtcatcat gcccagacga ggatctttaa tttcacgctg caggatgaga gcgatctctt
    40741 tttgcatttc ctgcgctacg cgctgcgggc gaccaaattc tttcgccata ataaattctc
    40801 ctgacaaaaa aggggctgtt agcccctttt taaaattaat ttcaggtgga agggctgttc
    40861 acgttggcct gataagacgc gccagcgtcg catcaggcaa tccatgcagg atgcgacata
    40921 aacgccttat cccgcatgga accctaaaaa ccttaagcga tagtacgttg gatctcgatg
    40981 atttcgaata cttcgatcac atcgccagtg cggacatcgt tgtagttctt aacgccgata
    41041 ccacattcca taccgttacg gacttcgtta acgtcatctt tgaagcgtcg cagggactcc
    41101 agctcgcctt cgtagataac cacgttgtcg cgcagaacgc ggatcgggtt gtgacgttta
    41161 accacacctt cggtaaccat acaacctgcg atagcaccaa atttcggtga tttgaacacg
    41221 tcacgaactt ccgccagacc gataatctgc tgtttcagtt ccggagacag cataccgctc
    41281 atcgccgctt tcacttcgtc gatcaggtta tagatgacgg agtagtaacg cagatccagg
    41341 ctttccgctt caatcacttt acgtgcagag gcatcagcac gtacgttgaa gccaaccagg
    41401 atggcgttgg acgccgcagc cagggtagcg tcggtttcgg tgataccacc tacgccagaa
    41461 ccgatgatct tcactttaac ttcgtcagta gacagtttca gcaaggagtc ggaaattgct
    41521 tccacagaac cctgtacgtc tgccttcagg acgatattca cttcgtgaac ttcgccttcg
    41581 gtcatgttgg cgaacatgtt ctcgagttta gatttctgct gacgcgccag tttaacttcg
    41641 cggaatttac cctgacgata gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg
    41701 gtaacttcat cacccgcagc cggtacgccg gacaggccaa ggatttccac cggaatggac
    41761 ggacccgctt ccagcacttc ctgccccagt tcgttacgca ttgcacgaac acgaccgtat
    41821 tcgaagccac acagaacgat atcgcccttg tgcagagtac cttcacgtac cagaacggta
    41881 gcaaccggac cacgaccttt atcgaggaag gattcgataa ccgcaccgct cgccatacct
    41941 ttacgtaccg ctttcagctc cagaacttcc gcctgcagca ggatagcatc cagcagttca
    42001 tcaataccgg tacccgcttt tgcagatacg tgtacgaact ggctttcgcc gccccactct
    42061 tctggcagga tgccgtactg ggagagttcg ttcttaacgc gatccggatc ggcttctggt
    42121 ttatcgattt tgttcacagc aaccaccacc ggaacacccg ccgctttcgc gtgctggata
    42181 gcttcgatgg tctgcggcat tacaccgtcg tctgcagcaa caaccaggac tacgatgtcc
    42241 gttgcctgcg caccacgtgc acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg
    42301 aaggtgatca tgccgttttc agtttcaacg tggtatgcac caatgtgctg ggtaatgccg
    42361 cccgcttcgc cagaggccac tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg
    42421 tggtcaacgt gacccatgat ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg
    42481 tcacggtcgc tcattaccgc ctcttccagc tcgttttcac gacgcaggat aactttgtgg
    42541 cccatctctt cagcaaccag ctgtgcggtt tcctgatcga taacctggtt gatggttgcc
    42601 attgcgccca gtttcatcat cgctttgatg acctgagagc ctttaaccgc catcttgttc
    42661 gccagttcgc caacggtgat ggtttcgccg atcacaacgt cacggttaac ggcctgagct
    42721 ggcttctgga agccttgctg cagcgaagaa cctttacgtt ttccgccctt accgccacgt
    42781 actgctgcgc gagcttcttc acgatcagct tttgattcag cgtgtttgtt gcctttcttc
    42841 ggacgcgctg ctttcgcgtt acgaccacgg ccacggccgc cttcgacttc acgatcgctt
    42901 tcgtcttctg cctggcgagc atgttgagaa gtagtgacgt gataatcgct ggaatcttca
    42961 gtcggttccg cgttatcagt ccatttgttt tcttccgcca tacgacgtgc ttcttcagca
    43021 acgcgacgtg cttcttcttc gagtttacga cgcgcttctt cttcggcttt acgcttgagc
    43081 tctgcagctt cctgctcacg gcgggctttt tcagcctggg cgtttttagt catatcgtct
    43141 tgttgattgc tcactttgtc tttttccgca gcttcacgtt tcgcttgttc agcagcttca
    43201 cgcttagctt gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg tttagccgat
    43261 tcttctgcct cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca
    43321 aggcgctcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt
    43381 tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt
    43441 tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt
    43501 ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc
    43561 tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct
    43621 gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta
    43681 cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga
    43741 tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg
    43801 aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca
    43861 ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc
    43921 agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca
    43981 taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc gaagtcttca
    44041 tcgatatcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta
    44101 gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg cgaagccaga
    44161 cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc aacggcgata
    44221 tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg
    44281 ttaatcacaa actgcgccgg gttatcatcc cacaggacaa tatcgatacg ctcgccgccc
    44341 agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga
    44401 tcgatacgtt tatcgttggt tttcaccgca attttcgcac gagatcccgg gtcgcgagcc
    44461 gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg gaacagttcg
    44521 atcagcattt ccggcttgga gcgggtgacg aacagctgcg cgccacgcgc ttccgggcga
    44581 acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata
    44641 tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg
    44701 tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc
    44761 accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggta
    44821 gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg
    44881 ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca
    44941 tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg
    45001 cgaacgtcga tttcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat
    45061 gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc
    45121 aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc
    45181 aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact
    45241 gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc
    45301 gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg
    45361 aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatgggg
    45421 tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg
    45481 atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg
    45541 atgccaacca gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt
    45601 tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat
    45661 tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata
    45721 accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc
    45781 cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga
    45841 tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg
    45901 cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg
    45961 gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccacgcc acattttgtc
    46021 aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt tgaatacttt
    46081 taccttctcc tgctttccct taagcgcatt attttacaaa aaacacacta aactcttcct
    46141 gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga
    46201 gcagaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc
    46261 tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca
    46321 gtgccgcact gctgtggatg cgacaaaagg gagctgttcc ttatgcatat actgcaaacc
    46381 tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg
    46441 cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta
    46501 ttcagtgtgg cgcatttcat aacaccactg gtggactgac ctatttcaac acgacgccgc
    46561 tgggccgcgc cgtgaccggc accatgctgg ttgctgctat gaaagaagat ggcgtgaata
    46621 tctggggtga cggcagcacc tataaaggaa acgatatcga acgtttctac cgttacggtc
    46681 tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg
    46741 aactgggtgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc gactacaaaa
    46801 tgtctgtcga aaaagcttac tccacggact ccaacatgct tggtgcaacg catgaagcga
    46861 aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccaattatg ggcgtgaagt
    46921 tttgggatga gagcgtgaaa atcccggcag aagaagtcac agtacgcttt gagcaaggtc
    46981 atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta
    47041 accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgaaaac cgtatcatcg
    47101 aagcaaaaag ccgcggcatt tatgaagccc ctgggatggc actgctgcac attgcgtatg
    47161 aacgtttgtt gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggtc
    47221 gtcagttggg ccgcctgctg taccagggac gttggtttga ttcccaggcg ctgatgctgc
    47281 gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttact ctggagctgc
    47341 gccgcgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag
    47401 agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt atcggtcaac
    47461 tgaccatgcg taacctggat atcaccgata cccgcgagaa acttttcggt tatgccaaaa
    47521 ctggcctgct ctcctcctct gccgcttcag gggtgccgca ggtggagaat ctggaaaata
    47581 aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt gattatcctc
    47641 tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaacccg
    47701 tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt
    47761 tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tgttgttttc
    47821 cgttgtgcga tccacgccat cccctagaac ggggctatac cagataaaca tcgggacatg
    47881 atacgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc
    47941 gcgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc
    48001 aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg
    48061 atcgtcctgc ggttgaaaca cagcgctgga ttgcggatag gcgctacaag ccggttcatg
    48121 gcttccattt aaatgaagaa caatcagttt cttttgctgc gtcttttgct gtaacgcttg
    48181 cgataaatgc ggcaacaaca attcatcaaa tcctctgaca tagactgttt ccatggcgcg
    48241 catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca
    48301 gaaagtctga aatcctgctt gattagccat attaataatg ttgtccgggt aattatgaat
    48361 gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg
    48421 tgcaccgctt attgcttgat taaacagttt gatctgcttt ctttgtgctt cgacttgcgg
    48481 tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac
    48541 aatcaacacg taggtatcaa tacctgtatc cctgactgat agttgaaaat acggcaccgt
    48601 gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat aattgagatt
    48661 gaaaaacggc gtataggtag caaatcgcga cgctagtata tatggactga acgcctcttt
    48721 attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg aaatgacaat
    48781 cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac
    48841 tgcaaaaaaa agaagggata aaaaggcaaa agcacataga taagggatat acattcccag
    48901 cattttgaca acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa
    48961 agttgtacca aagatacacc atgaatagag gctaatactc atatctgccg caatcagtaa
    49021 cacaaatggt acggcaataa gcaggcgaag cggaagggat gatatacgtt taacaaccag
    49081 gatggtcaga acgaagaaca gcaatcgctg aaccatatgc gctccggaag aagcgactaa
    49141 gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt taaaactttt
    49201 aacaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta
    49261 tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata tcagaccgaa
    49321 acactgttag attttttctt tttaaatgat gcggccattg cgttctcagt aattaaataa
    49381 cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt atactgacag
    49441 gggcaggatg gtttatcgag gagtaaacat atccgtattc gtaccggaaa tggagttgac
    49501 acaataaagt gccaattatg tcagcagaag ggaaaagctt ataactaagg cataccggca
    49561 ccatcgtttc aaggtaccag ctacgagtaa aacaactgga cgagatacag atacctgaca
    49621 accattcctc agaccaggac caaaacgaaa aaagacgctt ttcagcgtct ttctggaatt
    49681 tggtaccgag gacgggactt gaacccgtaa gccctattgg gcactaccac ctcaaggtag
    49741 cgtgtctacc aattccacca cctcggcacg gatactactt tttagttcgg gatatcgctg
    49801 gtcggcttag ccggagcagc tggctgagtt tgttcggttt tcgccggcgc actcaggttt
    49861 tcccattcgc tacctttatt ggttttgttg ctattgatgt tacccagcac cagactgatg
    49921 atgaagaata acgttgccag cagcgctgtc atgcgggtca tgaagttacc agaaccactt
    49981 gaaccaaaca gcgtagcgga agcgcctgct ccgaaggagg ctcccatatc agcgccttta
    50041 ccttgctgca gcatgatcag accaacaagg ccaattgcca caataaggaa aactactaaa
    50101 agagcttcgt acataatcaa cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc
    50161 aataaagcgg gagtttttaa tctttcccac tgaagcgggt gtgaatacta accaaagcga
    50221 atgaccttcg caagggcaat tttattgcat tgtatcaact gcggaaaaaa acagcaaaac
    50281 cgatgtgttc gctgagaaaa aaggcgacga tcgccgcctt tttagccagt tatctaacgc
    50341 tttaaacggc ttttactgca tcggcgatgc ggtgtgcaaa ttcagtcacc tgcgcttcgt
    50401 cttcgccttc caccatcacg cgaattaacg gttcggtgcc ggatttacgc aacagcacac
    50461 gtccacggct acccagcgcc gcttcaactt ctgcggtcac agctttgact gattcatgct
    50521 ccagcggatc gccgctacct gcggtgtaac gcacgtttac caggatttgc gggaacattt
    50581 tcatgccgct gcaaaggtcg tgcaggctca tatgattacg cgccatcgcc gccagcacct
    50641 gcaaaccagc aacgatgccg tcaccggtag tggttttatc cagcaggatc acatgaccgg
    50701 aattctctgc accgatacgc cagcctttct cctgcatttt ttccagtacg tagcggtcac
    50761 ccactttcgc gcgcgcaaat ggaattccta actgtttcag cgccagttca agccccatgt
    50821 tgctcatcaa tgtacccaca gcgccaccac gcagctggcc ctgacgaaga ccttcacgcg
    50881 cgatgatata catgatctga tcgccatcga ctttattgcc ttcatggtca accataatca
    50941 cgcgatcgcc gtcgccgtcg aaggcaatac cgagatccgc tttttcagcc agcacacgag
    51001 cctggagcgc gcgaacgtcg gtagccccca cttcggcatt gatgtttaca ccgttcggct
    51061 cacaaccgat agcgataacg ttcgctccca gttcacgcag cacgttcggc gcgatgtgat
    51121 aagtcgcacc gtttgcacaa tccaccacaa tcttcagttc actgaggcta agttcgtttg
    51181 ggaacgtggc tttgcaaaac tcgatatagc gacccgcagc atcaacgata cggctggctt
    51241 tacccagttc tgccgaatca acacagctga tctctttttc catttccgct tcgatggcct
    51301 cttctactgc atccggtagt ttggtgccgt cgatagagaa aaatttaatg ccattatcgt
    51361 agaacgggtt atgcgatgca gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca
    51421 gataagccac ggccggtgtt ggcatcggac cagtgaagag tgcggaaagg cccgcagccg
    51481 ccagacccgc ttccagtgct gactccagca tgtagccaga aatacgcgtg tctttaccaa
    51541 tgatgatctt acgggagccg tggcgcgcca gcactttacc cgcggcccaa cccagcttaa
    51601 gcacaaaatc aggggtgatc ggcgcatccc ctacacgacc acgaatccca tcggtaccga
    51661 aatatttacg attactcata gcgtttgttt tcctttgcag acagagtggc ttccaccacc
    51721 cgcatcgctt ctacggtttc tttgacgtca tgaacacgaa tgatatgcgc gccttgcata
    51781 gcggcaatga ccgcacaagc cagactaccg ctcagacgct cggacggccc cacgttcagc
    51841 agctgcccaa tcatcgattt tcgtgacata cccaccaaca gcggcaggtt gaaatggtga
    51901 aattcagcca ggcgcgccag taatgaatag ttatgggaga gatttttacc gaaaccgaat
    51961 ccggggtcga gcaacaattt ctcttttgcg atacccgcct gctcgcaacg tgctatttgc
    52021 tcaataaaat agcgattcac ttctgcaaag acatcgtcat acttcggggc ttcctgcatg
    52081 gtctttggat ttccctgcat atgcatcaga caaaccggta aaccggtttc tgcagctgcc
    52141 tccagagccc ctggttcgga aagggagcgg atatcgttaa taatgtgagc gccaactttc
    52201 gctgactcac ggatgacttc tggtttggat gtatcgaccg agatccagac ttcgaagcgt
    52261 tgagcaattg cctcaaccac aggaataaca cgttgcaact cttcttcaac gctaacttcc
    52321 gccgctcctg ggcgcgtgga ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc
    52381 atcagattcg catgtttcac cgcatctatc agcgagttat gcgtaccgcc atccgaaaag
    52441 gaatcaggcg tgacgttgag gatccccatt acgtgaggat ggctaaggtc cagtgaagtg
    52501 ccctgggcaa agagtttcat ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg
    52561 agcacgcccc ggggttttcg gtacaaatac agtcatctga tgcgggaact tatttgtcgc
    52621 ctaactgctc tgacatggtg ttacccgggt tcggcgtacg cggttcatca accggacgag
    52681 gagcctttgg actaccatta tcgccagaat tgttagaagc gcctggttct tcccagcccg
    52741 ctggcggacg tacatcgcga cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct
    52801 catatttcat gagagcatct ttcatcgcat gcagaatatc catattgtcg gtcagaagct
    52861 gacgcgcacg attatagtta cgctcaatca gtgctttcac ttcctggtcg atgatacgtg
    52921 cagtttcatc ggacatatgt ttcgctttcg ctacgctacg gccgaggaac acttcacctt
    52981 cttcttccgc gtacagcagt ggacccaatt tctcagagaa gccccactga gtcaccatgt
    53041 tacgtgccag gttggtcgca actttaatat cgttggacgc accggtagaa acatgttccg
    53101 gcccgtagat gatctcttcc gccagacgac caccgtacag cgtagaaatc tggctttcca
    53161 gtttctgacg gctggcgctg attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg
    53221 cacgaccgcg tgggataatc gtcactttgt gcaccggatc gtgttccggc accaggcgac
    53281 cgataattgc gtgacccgct tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca
    53341 ccatggagcg acgttccgca cccatcatga ttttgtcttt cgctttctcg aactcaacca
    53401 tcgacacaac gcgtttgttg ccacgagcag cgaacagtgc cgcttcgttc accaggttcg
    53461 ccaggtcagc accggagaaa ccaggagtac cacgggcaat gattgccgcg tcgatatcgg
    53521 gtgccaatgg tacgcgacgc atgtgaacct tcaggatctg ctcacgaccg cgaacatctg
    53581 gcaagccaac cacaacctga cggtcgaaac ggccaggacg cagcagcgct gggtcgagaa
    53641 cgtccggacg gttagtcgcg gcgataacga tgataccttc gttaccttcg aagccatcca
    53701 tctcaaccag catctggttc agagtctgtt cacgttcatc gtgaccaccg cccagacctg
    53761 cgccacgctg gcggcctacg gcgtcgattt catcgataaa gatgatgcac ggtgccgctt
    53821 tcttcgcctg ttcgaacatg tcacgaacac gggatgcacc cacaccgacg aacatttcta
    53881 cgaagtcaga accggagata gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg
    53941 ccagcagcgt tttaccggta cccggaggac cgaccatcag gacgcctttc gggatcttac
    54001 cgccgagttt ctggaagcgg ctcggctcgc gcagatactc aaccagttca gcaacttctt
    54061 cttttgcttc gtcgcagccc gcaacgtcag caaaggtcgt tttgatctga tcttcagtca
    54121 gcatgcgcgc tttgctctta ccaaacgaca tggcaccttt gccaccgccg ccctgcattt
    54181 gacgcatgaa gaagatccag acaccaatca gcaacaacat cgggaaccag gagatgaaga
    54241 tagaagccag caggcttggt tcttcaggcg gttcaccgac aaccttgacg ttcttggtca
    54301 acaggttatc cagtaatttc ggatcctgaa ccggaatgta agtggtataa cggttactat
    54361 ctttcttggt aacgttgatt tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat
    54421 tgacctcttg taggaaggta gagtaatcca ccttacggcc attagactcg ctgggcccaa
    54481 agctctggaa tactgacatc agcacaacgg caatgaccag ccagagtatt aggtttttcg
    54541 ccatgtcact caagggatta acctcttatt acaactgtgt taaaaacagc gtcaggatac
    54601 tctatatcca gcatctttca aactttcgtc tgaaatctcc cggttagggt ttacgcccgg
    54661 tcgctacaat atacacttcc cgcgaacgtg cacgagaaga gtccggctta cgaactttga
    54721 ccttcgtaaa cagggagcga atttccctta gatactcatc gaaaccttcg ccctggaaca
    54781 ccttcactac aaaactgcca cctggcgcta atacatcacg acacatttct agcgccagtt
    54841 ccaccagata catggcacgg gggatatcca ccgccggtgt tccgctcatg tttggtgcca
    54901 tatcggacat gacaacctgg actttgctgt cgccaacgcg ctccagcagt gctttcatca
    54961 ccagttcatc acgaaaatcg ccctgaagaa agtccacacc aacgatagga tccataggta
    55021 aaagatcgca agcgatgatg cggcctttgc cgccaatttg gttgaccaca tattgtgacc
    55081 aaccacccgg agcagcacca aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac
    55141 tttgctgtat ttcatcaagt ttaaaccagg cacgggaacg taaccccttt ttctgtgcct
    55201 gttgaacata tttatcgcta aagtgttcct gaagccagcg gctggagctg gcagaacgct
    55261 tcttacctgt catttaactt tcccatgggg ataactcatc gtaaccaatt gcgtaaattt
    55321 ttacgcgcct atttggtgat atatgggaga tggcggtaga atgacccgtt ttcaatccca
    55381 acgtaagcaa aaaatacgat gaatctgagt actaaacaaa aacagcacct gaaaggtctg
    55441 gcacatccgc tcaagccagt tgttctgctt ggcagtaatg gtttgaccga aggggtgctg
    55501 gccgagattg aacaagcgtt agagcaccat gaactcatca aggtgaaaat cgccaccgaa
    55561 gatcgcgaaa ctaaaacctt gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat
    55621 gtacaggtca tcggtaaaac gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg
    55681 ctgccacgct aagattatcc taaagttaca cacattcgct gtgtaaaacg aggggttttc
    55741 cgcaggcagg agagcaaaat gccacgctct gttcgttgat aaaaggccgc atagcggcct
    55801 ttttcctttc tttacaatac atcaacatct tgagtattgg gtaattctta caggtattcc
    55861 accttaatta cttcaaattc tacttcgccg cccggcgttt tgatgaccac aacatcatct
    55921 tcttctttgc cgatcaggcc acgagcaata ggcgagttta cggaaatcag gttttgttta
    55981 aagtcagctt cgtcatcgcc aacgatgcga taagtctgtt cttcgtcaga atcgagattc
    56041 agcaccgtta cggtagcacc aaaaataacg cgcccattgt tgggcatttt ggtgacatca
    56101 atcacctgcg cgttcgacag cttggcttcg atgtctttga tacggccttc gcaaaagccc
    56161 tgctgttcac gagctgcgtg gtattcggcg ttttctttca ggtcgccatg ctcacgcgct
    56221 tccgcgatag cagcaatgat ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg
    56281 cgtaattttt cagcgccgcg taaggtcatc ggaatagctt gcatttgtta tacctcttga
    56341 atattcctga tggggcaagt cttcacccac cccggctgtt cggcctgccc ggcataactc
    56401 ctcaccggga ccagaagcaa aaaaataccg acccgggtac aagtcccagg tcagctacaa
    56461 ttcacatttt gatagtcatt ttaccctgaa gttcccgaag ggtcatcgtt tactttatag
    56521 ggcgttgcgc cgtagtatga cggctcgatt ccaggttgtt agcgcgagat tatgcgattt
    56581 tccagattta tcatcggatt gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt
    56641 gatgagtaca ttactcaact ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc
    56701 ggcgcgtcgg cccccgctat tgattaccac agtcagcaga tggcgctgcc cgccagtacg
    56761 cagaaagtga ttactgcact ggcggcgttg attcaactcg gccccgattt tcgttttacc
    56821 acgacgcttg aaaccaaagg caatgtggaa aacggcgtac ttaagggtga cttagtggcg
    56881 cgatttggtg ccgatccgac gttaagacgt caggatattc gcaatatggt cgcgactttg
    56941 aaaaaatctg gcgtcaacca aatcgatggc aatgtgttga tagatacctc cattttcgcc
    57001 agccacgata aagcccccgg ctggccatgg aatgacatga cacaatgctt tagcgctccg
    57061 cctgccgccg ccatagttga ccgcaactgt ttctccgtct cgctctacag tgccccaaaa
    57121 cctggtgata tggcttttat acgcgtggca tcttattacc ccgttacgat gttcagccag
    57181 gtacgcaccc tcccccgtgg ttctgccgaa gcgcaatact gcgagctgga tgtggtgcca
    57241 ggcgacctga accgctttac gctgaccgga tgcctgccac aacgttctga gccgctcccg
    57301 ttggcttttg ccgtgcagga tggagccagc tatgccggtg caattctgaa agatgagtta
    57361 aaacaggcag gtatcacctg gagcggaaca ctgctgcgcc agactcaggt taacgaacct
    57421 ggaacggtag ttgccagtaa acagtcggcc ccgctgcacg atctgcttaa gattatgctg
    57481 aaaaagtcgg acaacatgat cgccgatacg gttttccgca tgataggcca tgcgcgcttc
    57541 aatgtgcctg gaacatggcg ggccgggtcg gacgccgtgc gtcagatcct gcgccagcaa
    57601 gccggtgtcg atattggaaa caccattatt gccgatggtt cagggctttc gcggcataac
    57661 ctgattgccc ccgccaccat gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa
    57721 cttaacttta tctccatgct gccgctggcg ggctatgacg gctctttgca gtaccgtgca
    57781 ggtctgcatc aggcgggcgt ggatggaaaa gtctcagcga aaaccggttc gttgcagggg
    57841 gtatataacc tggcgggatt cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa
    57901 tatctttctg gctatgcagt agaaccggcg gatcagcgta atcgccgtat tccgttagtg
    57961 cgttttgaaa gccgtttgta taaagatatt tatcagaata attagcctaa agagaaaccc
    58021 cggcacatgg ccggggtttt gctgattaac gcttgtaaat gaactcaacg ccttcttcgt
    58081 cgtcttcgtc ccagtcgtca tcccagtcct catcatcctc ttcagcaatc tcttcaagct
    58141 gctgacggtg ataatcatcc cacatgaatt cgactttctc tggctgtttc gcttcttcag
    58201 cctgcacgac cgggttttca atgataaagg tcatcacatc ccagcagaga tctttcacgc
    58261 ccagtccgct cgccgcagag atcagataat atttatcttc ccaacccagc gcctcagcga
    58321 tcgctttcgc tttctcttcg gcttctgcct tatccagcag gtcgatcttg ttgaagacta
    58381 accaacgcgg tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac
    58441 gcgcgttttc aaccggatcg gtgccatcaa tcggatcgat atcgatgagg tgcaacagga
    58501 cgcggcaacg ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct tctgcagcgc
    58561 cttcaatcag tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc atacgcacca
    58621 cgcccaggct tggtaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg
    58681 ataccgcacg aataaaggtc gatttacccg cgtttggcat ccccaacata ccgacgtcag
    58741 ccagcagcat cagctccagc agcagctcac gcttatcgcc cggcgtaccg ttagttttct
    58801 gccgtggtgt acggttaact gacgacttga aacgggtatt gcccagaccg tgccagcctc
    58861 ccttagcaac cagcagacgc tgaccgtgtt tggtcatatc gcccatggtt tcaccggtac
    58921 cctggtcgat tacacgcgta cctaccggta ctttaatcgt gacgtcttta ccgcgcttac
    58981 cggtacagtc tcggcttgca ccgttctgac cgcgctctgc acggaaagat ttttcaaaac
    59041 gataatcgat aagcgtgttc aggttctcgt cggcttccat ccatacgtca ccaccatcac
    59101 cgccgtcgcc gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc
    59161 aaccattacc gccatcacct gcaacgacca gaatcgatgc ctcatcaaca aacttcattt
    59221 tattctccgt aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta
    59281 atccgcccca aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg
    59341 caccgagata acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg
    59401 ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct
    59461 gcgctgcctg ccagcgagcc atcgcttccg ccagggcgcc atatcctacc aatgtattca
    59521 gtccgcaaaa aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggat
    59581 ctgccagagg gaagagcgca attgtacata aagtgtacag taaaaacagg atctgcggtg
    59641 aggccagccg acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgccg
    59701 caccgacccc aaagataact ccccaggtgt aatcggtgag ctttgtaaat atctcgacca
    59761 gactggtgtt aaaaaacatc accagaccgc tcaggagcat caatgcccct acaacctgag
    59821 tactgcgcat tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga
    59881 gttgcccaat cacctgcgaa gcggtcggac tcaagtattg caaggatgag ctgaacagga
    59941 tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac
    60001 gaaacacgcg taatggcggc aaccgcttct tcaccgcaag aatggcacca aggccaatac
    60061 tcgccatcaa gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct
    60121 tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa
    60181 tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca
    60241 ccatgcgcat ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt
    60301 catccgttac cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt
    60361 tcgggccttt aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac
    60421 gaccgcaacc tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc
    60481 tacccgccag aacggattcg ccaccgaaac gcttaacgcc caggcgttta gcttctgaat
    60541 cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag
    60601 gtcttaggcg ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg
    60661 cttacgatag tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg
    60721 agcaacaact tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc
    60781 ttcaccgttt gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc
    60841 cagcttttcc aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg
    60901 gaaaaccgcg tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc
    60961 gctataaata ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc
    61021 tacagtcaat acacgaagaa aaaaaacaca acttgtacgg taacgtttat ctgtgccatt
    61081 ttttcagtac aatcacccta tattcctaac cataaaccct aagttgcctt tgttcacagt
    61141 aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat caatgagtta
    61201 accgcgcaag atatggcggg tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc
    61261 caactgatca atcagttagg ctattacatc gtcagcggcg gcggtaaacg tattcgtccg
    61321 atgattgctg tactggctgc acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt
    61381 gctgccctga tcgagtttat ccacacggcg actctgctac acgacgacgt tgtggatgaa
    61441 tcagatatgc gcaggggtaa agctaccgcc aatgccgcat ttggcaatgc cgccagcgtg
    61501 ctggtaggcg attttattta tacccgcgcg ttccagatga tgaccagcct cggttcactc
    61561 aaagtgctgg aagtcatgtc agaagccgta aacgtcatcg cagaaggtga agttctgcaa
    61621 ctgatgaacg ttaacgatcc ggacatcact gaagaaaact acatgcgcgt tatctacagc
    61681 aagactgcgc gtctgtttga ggctgccgcg cagtgttccg ggattctggc tggctgtacg
    61741 ccggaggagg agaaaggcct acaagattat gggcgctatc tcggcactgc tttccagttg
    61801 atcgacgatt tactcgatta caatgccgat ggcgaacagt taggtaaaaa cgtcggcgac
    61861 gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca tggcacacca
    61921 gaacaggcac agatgattcg taccgccatc gaacagggta acggtcgcca tcttctggaa
    61981 ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca gcgtgccgag
    62041 gaagaagcag acaaagccat cgcagcgtta caggtgctcc cggacacccc gtggagagaa
    62101 gcactcgtcg gcctcgcgca catcgctgtt caacgcgatc gttaatcccc tcccctcatc
    62161 ccgcgcagtg cgcgggatga ttccagtaaa ttccataaaa cacttattca gctctataac
    62221 tcctgaaaaa cggggctgtg tatctttata tattctgaat attcacactc tttacaggaa
    62281 ctttttagag caataggcca tcaggagtat agtgatgctc gactgaaaca gaagaagtgt
    62341 tctgaatgaa agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg
    62401 gcatcccgct gacatcattg caggtttgcg caagaaggga acgtcaatgg cggcggagtc
    62461 tcgcagaaat ggtttgagtt cctcaacgct ggcgaacgca ttatcacgcc catggccgaa
    62521 aggagagatg attattgcga aagccctggg aactgacccc tgggttatct ggccatcacg
    62581 ctaccatgat ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac
    62641 taaaccgaaa aaatgagtgg cctggcggta gccccgcgaa cggggctgcc agctctcaga
    62701 caattactcg cctttcacac gctcaatatt tgcacctaaa gcgcgtagtt tgtcttcaat
    62761 gcgttcgtag ccacgatcga tgtgataaat acgatcaacc accgtcgtcc cttccgcaat
    62821 acagccagcc agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc
    62881 agaaagtttt tcaacaccgt gacaaataac ggtattgctt tcgatttcgg cgtgcgcgcc
    62941 catacggctc agctctggca catgcataaa gcggttttca aagaccgttt cggtgatgaa
    63001 tccggtccct tctgccacca gattcaacag cgtgaactgg gcctgcatat cggtagggaa
    63061 tgccggatgc ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag
    63121 gctaatccag tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac
    63181 ggcgtccaga gtatctggct gcgcgttacg gcagataatt ttgccgcgag agatcgccgc
    63241 cgccaccagg aaagtaccgg tttcgatacg atccggcagc acgcgataga caccgccgcc
    63301 taaacgttcc acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc
    63361 cagcgtaatc aggaagttcg cggtatcgac gatttccggt tcacgcgctg cgttttcaat
    63421 aatcgtggtg ccttccgcaa gggttgcagc acacatgatg gtcaccgttg cgccaacgct
    63481 gactttatcc atcacgatat gtgcgccttt caaacgacca tcgacggaag ctttaacgta
    63541 accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac
    63601 cggacgtgcg ccgatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc
    63661 taccagcggc cccagcgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc
    63721 gcagaataca ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc
    63781 acccagctgg cttagcagct tcattgatgt atcgacgtct tttagtttcg ggacgttctg
    63841 gatctctacc ggttcttccg ccagtagtgc ggcaaaaagg ataggcagag cagcattttt
    63901 agcgccggaa attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc
    63961 catttagttt gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca
    64021 aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac
    64081 agcatgaatg cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt
    64141 tttaacccga ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc
    64201 atcgccggaa acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc
    64261 attattttcc atgggatctt caatcatcag ttaataaatc agcgaaatat cttagagcaa
    64321 agttgcgctg gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg
    64381 caggcaggtt tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg
    64441 cagaacatca gcaggcaaat tatacaattt cgccagggta tacactttgt cgtttacccc
    64501 ctgaagcgtc acattgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc
    64561 cagtccaccc gtatccacgc gagacacacg gctaagatcg atgctggtaa tccccttcac
    64621 cgcttcctca cgtatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa
    64681 cgccagcgtg tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc
    64741 agagtgattt tctgttgaga aatcgatttc agttgcgcag tcaggccgtc gatacctttg
    64801 gtacgcagca gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca
    64861 atcatgtcgt aagcctgcca gttgcccgtc tgggagtttt tacgccactg gaagtccaga
    64921 cgcaccggcg ggcggccatt cgggtcaata atggtaacgc gaatcggcac aatggtttta
    64981 tcacccagcg gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc
    65041 tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa agtaagcatc acgttgagct
    65101 ggggtcgcac tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg
    65161 tatggcaaca gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgc
    65221 ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttatac
    65281 ggattggtct ggtctgccgc ggttgccgca ctcagcggtg caatcaccag caaagcgacc
    65341 atcattaaac gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt
    65401 tcagtggttt cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca
    65461 tcgcctttac taccgtaaag gaactgacca atgagatctt ccagcaccat cgcagacttg
    65521 gtgtcctgaa ttgtatcgcc atccttcagg atagcagttc ccagttccgg gtcttcaaaa
    65581 ccgacgttta atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa
    65641 ctggtatcag gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag
    65701 gttttcgggt ccagcgtaat atccgccacc cggcccacaa caacgccacc aatgctgacc
    65761 ggagagcggg ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt ataggtcggt
    65821 tcagtacgta tagacgtcac gttcgctgcc ttcaggcaaa caaacagcgc cgcaagcagt
    65881 gctgctaata aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt
    65941 cccaaacatc aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac
    66001 aacggtgcga gtggttgccc ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt
    66061 aaacaacgaa atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac
    66121 gagatccata cgccagtcga cggcattttg cattgccgac cagaagaacc cactatcaat
    66181 gcctttccaa ctgacgccga ccagcgatcc gccccagatc cccacggcga cgaagataac
    66241 cgtcaacagt ggtaatgaaa taactccagc ccagaaacgg ggagaaataa cccgacgcag
    66301 cggatccacc gccatcatct ccatactaga gagttgctct gtagcgcgca tcaggccgat
    66361 ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc
    66421 cagttcacgc agtagcgata acgccaccag catacccaga ctggtttccg cactataagt
    66481 ggtcagaacc agataacctt gcagccccaa caccattccg atgaacacgc cagaaacaac
    66541 aataatcagc atcgacagga cgccgacatt atagagctgg cgcaccagca gcggtgcatg
    66601 tttgcgaaat tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa
    66661 cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag
    66721 tggcttaact ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa
    66781 caggcccgtc agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca
    66841 acgcctgggc actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatccg
    66901 caatacttaa cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc
    66961 tgttcagctc agaaatcagc ttcaccagca cgcccatggt aatgggatct tgcccaacaa
    67021 agggctcatc aaacatgatg agatccggct ccagcgcaat cgcacgtgcc agcgctgcac
    67081 gccgcgccat cccaccggaa agttcagaag gcataagttt agccgctcca cgcagcccca
    67141 cggcctccag cttcatcatc accgtactat gcaacaatgg cgcgggaagt tgggtatgtt
    67201 cccgcagtgg ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gccccggact
    67261 gaaataacat gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa
    67321 tattctcacc atcgaaaagg atctcaccat gatctggtgc gatttgcccg ccaatcagac
    67381 ggagtagcgt cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg
    67441 acacggtcag ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc
    67501 gcatatcgac taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt
    67561 ttctaagcat ggcgctcaat ttaaccttga acccaacata tttacagaat attacctgcc
    67621 gtggttagcg aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa
    67681 tctgttacgg ctttctgaaa atcttcagcg gaccggcgag tatacctgaa gaaaggacgt
    67741 tagatgcttt tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc
    67801 gaccgcctgg tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgttgatc
    67861 atcggcatga cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc
    67921 gcgtctttgc acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc
    67981 aatatattgc tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattctgat
    68041 gttctacgcc gtgaattacc cttaatgttg ttggtcagcg tggtggccgg ttccgtactc
    68101 tatgacggac aacttagtcg cagcgatggt atctttctcc tctttctggc tgtgctatgg
    68161 ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc
    68221 agagagcagc ttgcagagct gccgcgtgaa ggtggattgc ccgtcgcatt tttatggctc
    68281 ggcatcgcac tgatcattat gccagtgacc acgcggatgg tagttgataa cgccacggtg
    68341 ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga
    68401 accagcctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt
    68461 gcggtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc
    68521 gcgctgataa cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg
    68581 ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt
    68641 ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta
    68701 tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg
    68761 ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga
    68821 gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa
    68881 agggaaagtt gtcgtcatgg ggatgggcaa atcggggcac atcgggcgca aaatggcagc
    68941 aacatttgcc agcaccggta caccttcatt tttcgtccat cctggcgaag ccgcgcatgg
    69001 tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc
    69061 cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat
    69121 caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt
    69181 ggcgaaagaa gcctgtccgt tagggctggc accgaccagc agcacaaccg ccacgctggt
    69241 tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt
    69301 tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat
    69361 tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt
    69421 gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat
    69481 tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg
    69541 tcagttaagt attgccgatg tgatgacgcc ggggggaata cgcgtgcgcc caggcattct
    69601 ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga
    69661 tggcgaccat ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta
    69721 aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc
    69781 cctgtcagcg ccgacgttat ggcaaaagca gagaacattc gtctgctgat cctcgacgtt
    69841 gatggcgtac tgtcagatgg cctgatttat atgggcaata acggcgaaga gctgaaagcg
    69901 ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct
    69961 atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact
    70021 cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg
    70081 gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg
    70141 gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc
    70201 gattacgtaa cgcacattgc tggcggtcgt ggcgcagtgc gagaagtttg cgacttatta
    70261 ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac
    70321 gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt aatatggccg
    70381 aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc
    70441 atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac
    70501 acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt
    70561 ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg
    70621 accggatgct ctatttatat ggacacgttg aagtcaatgc actcgtgccg gactctcaac
    70681 ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg
    70741 aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca
    70801 acttacgcaa caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa
    70861 acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg
    70921 gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct
    70981 cttgatatgc aaggcaacgt ggttaccttt accggcaatg tcatcgtcac ccagggcacc
    71041 atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa
    71101 gtgattgacg gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc
    71161 gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg
    71221 ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg
    71281 gtgaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg
    71341 gtgccgtcgc agctgcagga caaaaacaat aaaggccaga ccccggcaca gaagaagggt
    71401 aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt
    71461 cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg
    71521 ccaaacggtg ccggtaagac caccactttc tacatggttg ttggcattgt gccgcgcgat
    71581 gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg
    71641 cgccgcggta tcggctatct gccgcaggaa gcctccattt tccgtcgcct cagcgtttac
    71701 gataacctga tggcggtact gcaaattcgt gatgacttgt ctgctgaaca acgtgaagac
    71761 cgcgcgaacg agttgatgga agagtttcac attgagcacc tgcgtgacag catggggcag
    71821 tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg
    71881 aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt
    71941 aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg tactgatcac tgaccacaac
    72001 gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc
    72061 gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg
    72121 gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc
    72181 tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc
    72241 tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc
    72301 aggcgctgga gagtaatccg ctgcttgagc aaatcgatac tcatgaagaa atcgacaccc
    72361 gcgaaacgca agacagtgaa acactggaca ccgccgacgc gctcgaacaa aaagagatgc
    72421 cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg
    72481 gcaccagcgg tgactacatt gacgacgagc tgccggtcta tcagggcgaa acgacacaga
    72541 ccttacagga ttacctgatg tggcaggtcg agttgacacc gttttccgac actgaccgcg
    72601 ctattgctac ctctatcgtc gatgccgttg atgacaccgg ttatctgact gtcccgctgg
    72661 aagatattct cgaaagtatg ggcgatgaag agattgacat cgacgaagtt gaagccgtcc
    72721 ttaagcggat ccaaaggttt gatccggtcg gtgtggcggc aaaagatctg cgtgactgcc
    72781 tgctgatcca actctcccaa ttcgataaga ccacgccatg gctggaagag gccagactga
    72841 tcattagcga tcatctcgat ctattagcca atcacgactt ccgcacttta atgcgcgtca
    72901 cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc
    72961 gccccgggca gtcgatccag actggcgaac ctgaatatgt cattccagat gtgctggtgc
    73021 gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg cgtttgcaaa
    73081 tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgat agccagttta
    73141 tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata
    73201 cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggcg
    73261 aagaatatat gaaaccgatg gtactggctg atatcgccca ggccgtcgag atgcatgaat
    73321 cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac
    73381 tgaagtattt cttttccagt cacgtcaata ctgaaggcgg gggtgaagcc tcttccacag
    73441 cgattcgcgc actggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg
    73501 acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg
    73561 cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac
    73621 ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc
    73681 gaggcactgc gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga
    73741 atcaaccagg tctatgttgt tctgaaagtg gagaaggtca cccacacctc agatgcaaca
    73801 ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc
    73861 attgatggct taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa
    73921 caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg
    73981 ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt
    74041 gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt
    74101 acgcgaagcc gcgtccactg ccagagcaaa aaacgcgccc tggaaatcat cagcgagctg
    74161 gcggcgaaac aacttagcct ggcgcctcag gtggtttttg aagctatcct gacgcgggaa
    74221 aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa
    74281 gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa cacctatagc tttcgatgcc
    74341 atcgacaacc aaccggtgga cttacttttt gccctgctgg tgccggcaga ccaaactaaa
    74401 acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc
    74461 cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt
    74521 actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag
    74581 tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt
    74641 gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat
    74701 ctggctcgaa ctctggccga tcgagagatt tctgccgccg tcagcattga tgttcgtaat
    74761 atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca
    74821 ccgcaactac tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg
    74881 cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa
    74941 agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg
    75001 tccgttcacg agctggcaga aatgctgcgt acccgtctgc tgggtaaacg tgaacgtgaa
    75061 ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac
    75121 gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg cccaatgaca
    75181 ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc
    75241 taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc
    75301 tacctgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag
    75361 caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcggacgctg
    75421 gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc
    75481 atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt
    75541 tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt tattgcgctg ctgatgttgg
    75601 attctgccaa aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac
    75661 tggccgccgt tatcgccctc tttaactctg gttttgatga agattaatct tcatcacttt
    75721 gacattcaaa tcccttcaag ctcccccggc aataagaaaa gcgcgtactg aaatcgttat
    75781 caatcaacgt cgattgtaca tttcgtgcgc ttttttactt tcagaagaac cttaagaaaa
    75841 ccttaagaag cattgtttag atttcgttta agttaatcga ccatactgga gatcgtcaga
    75901 aaatatttcc aggagatggc atgattcgct tatctgaaca aactcccctg ggcaccggaa
    75961 gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc
    76021 atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg
    76081 gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt
    76141 gcggaaccgg ttatgtctac gatgtgattg ccgatttcga cggcaagcct tccattacgc
    76201 tgaccgaatt tgcagaacaa tgccgttacg aagaagacat tgcacaactg cgccagttac
    76261 tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc
    76321 agaatatcct ctgtcaccgc atcagtgagt cagaggttac ccccgtcgtc tgcgacaaca
    76381 ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc
    76441 aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag
    76501 atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatcaagct
    76561 ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct
    76621 gacggctacg cacgtagccc gatggcgcgg agactttgaa acgaagtgga ttaggtaata
    76681 cagctgccag caatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac
    76741 gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa
    76801 ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat ccggcttcca
    76861 gcccttttcg aacccagcta cgcccatccc ataaaaagag atttttggct gtctgctgag
    76921 aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggcttgct
    76981 caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct gcggcaatca
    77041 ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt
    77101 aacgaaaatt gccatgcagc caggcgctga cttgccgctc gaccattacc gctgagaaag
    77161 gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaatggcg aggacaacca
    77221 ttaaccgcag aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc
    77281 agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt
    77341 ctgcgccagc atatatgccg gggtggtgac aatcttattg tcttcatcaa ccacgatatc
    77401 atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg cggtatcgat
    77461 atcagtaccg atggtcaaac gtagcgggaa atcgaaaatt ttcggcagta tcgccggtgc
    77521 aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa
    77581 ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat tcttcgccgc
    77641 gccaaaacct cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg
    77701 acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg tttccgtcat
    77761 cgcttcacca gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac
    77821 cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaat accgcttcat gaatttcaga
    77881 accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcttcct
    77941 ttcgcaattg actgaaacac attaattttt ttaataaaaa tgctacgcat cacacattta
    78001 actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa
    78061 cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt
    78121 gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca
    78181 cccacgcttt cagtacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt
    78241 caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga cccagctgtt
    78301 gcagatggcg taaccccact gaccccgctg cacctttaat tttatgccct tcctcaacaa
    78361 tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca
    78421 tcctctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact
    78481 gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg
    78541 tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa
    78601 cagaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct tgcttgtctt
    78661 tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga taacgcttcg
    78721 tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc agcaacacca
    78781 ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg gcgacatcaa
    78841 cgctgttacc taacttttcc agcacagagc gcgcgacaat cacgttcagt tcaatgtctt
    78901 ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcaa
    78961 cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt
    79021 cgctggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac
    79081 caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg
    79141 cgaagatttt atccagctca tcctgcggaa tgccgatacc agagtcttcc acttcaaaat
    79201 gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgagtgaatt
    79261 tgacggcgtt actgatgagg ttccacaaga tctgccgtaa acgcgtcccg tcggtaatga
    79321 cctgatgcgg taatggcagc gtcggctcca gattaaagcg caatcctttt tgttgcgcct
    79381 gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggctgattat
    79441 caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgttg aagatattcc
    79501 ccaacgtgac agccgaaaca tggatggttt tgagatattt ttcctgctcg gcggtgagtt
    79561 cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta cgcaattcgt
    79621 gactgatggt ggagataaac gtcgttttgt cgcggctggc gcgctcaagc gcatcctgat
    79681 accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt ttacccacgc
    79741 ggtcgtagta cggtacttta cggatttcaa agcaggcttt gcgtccgtcg ggataatcca
    79801 gccactgctc ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga
    79861 ctttcgcagc ggcttccggt gagtaaacat cagcaggttt caggtgaacc agttgttttt
    79921 cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt
    79981 tacgataaaa aaccaggtcg ggtgaagcgt caaggaagga acgtaagaat gaggattgct
    80041 gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg
    80101 tttcctgcag ttctgcttcc gctttttcac gaacggcgat ttcctgattt agctgggcaa
    80161 tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt
    80221 gcaccagtcg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa
    80281 agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata acatcaatgc
    80341 tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga
    80401 cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa
    80461 cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc
    80521 cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc
    80581 ctcaatggcc tacgccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc
    80641 atgttcattc agataacgat ccagctcgac cattcccgtc catcgatttt cgcaccacag
    80701 cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg
    80761 tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa tcctccagtt caattccgtt
    80821 caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcactatat gcagcggatg
    80881 cagctttatg ccgtccacgc cggtttcaac cactcgctcc agcgtttgca aacattcagc
    80941 ctgcccttcg ccaggcaggc caacaattaa gtgagaacac accttcagcc cacgctcacg
    81001 cgccagctgc gtcgtgcgct gatagcaggc aaaatcatga ccgcgattga tgcgatgcag
    81061 tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtagc cctggtcctt
    81121 atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg taccaacaca
    81181 caaaccgaca atattggcct ggctcaccgc ctgctgatac atcgaacgca gcacctgaac
    81241 ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag cgcggttaac
    81301 taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg cttcatcggc
    81361 aaacgaggca acattacaga atgtgcagcc gccacgcccg atggtaccgt cacggttagg
    81421 gcagctaaaa ccgccatgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag
    81481 atcaccacca aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg
    81541 aaaagaggcc aaagcctgcc atttttagcc caattcggcg atgacctgga tcaatcgtcc
    81601 cggcctgctt ttatcaaatg cataataaat caaaattacc gaaatttcat gcacaatcac
    81661 ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa
    81721 aaacagtagc atgaaacgtt attaccaatt aaggcagtat aaaatgctgg ctttctcgtc
    81781 aattcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaactg gctgtttctc
    81841 taacgacgcc ccttttagcc ttaaatagaa aatccaattt aatttcagat aattaataaa
    81901 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
    81961 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
    82021 tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg
    82081 ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt actgtgcccg
    82141 tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
    82201 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
    82261 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
    82321 gcgcgattct cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg
    82381 atcgtttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa aactacgccg
    82441 tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg
    82501 aagaagagct gcaacgcgaa acgttgtcga ttgtgggctg gcgtgatgtc cccactaacg
    82561 aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga
    82621 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
    82681 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg
    82741 tcaatattta taaaggtctg tgtatgccgg cggatctgcc gcgcttctac ctggacctcg
    82801 ctgacctgcg tctggaatcg gccatctgcc tgtttcacca gcgcttttcc actaacaccg
    82861 taccgcgctg gccgctggcg cagccattcc gctacctcgc gcataacggt gaaatcaaca
    82921 ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctga
    82981 tccctgacct gcacgacgcc gcgccgttcg tcaacgaaac cggctctgac tccagttcga
    83041 tggataacat gctggaactc ctgctggcag gcgggatgga tatcatccgt gccatgcgtc
    83101 tgttagtgcc accagcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
    83161 ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcaggcatc gtgatgtctg
    83221 acggtcgttt tgccgcctgt aacctcgacc gtaacggtct gcgtccggca cgctacgtca
    83281 tcaccaaaga taaactgatc acctgcgcct ctgaagtcgg tatctgggat tatcagcctg
    83341 acgaagtggt cgaaaaaggc cgcgtagggc caggcgaact gatggtgatc gacactcgca
    83401 gtgggcgcat tctgcactcg gcagaaaccg atgatgacct gaaaagccgc catccctata
    83461 aagagtggat ggagaaaaac gtccgccgtc tggtaccgtt tgaagatctg cccgatgaag
    83521 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
    83581 acagcgcgga agagctggat tcggtgatcc gcgtactggg tgaaaacggt caggaagcgg
    83641 tcggttcgat gggcgacgac accccattcg ccgtgctctc cagtcagccg cgcattattt
    83701 acgactactt ccgccagcaa tttgcccagg tgactaaccc gccaatcgac ccgctgcgcg
    83761 aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttctgcgaag
    83821 cagaaggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctat tccgatttca
    83881 aacagctcac gacgatgaaa gaggatcact accgcgcaga tacgctggat atcacctttg
    83941 acgtcactaa aaccacgctc gaagcgacgg tgaaagaact gtgtgacaaa gccgaaaaaa
    84001 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
    84061 tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
    84121 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccatttcg
    84181 ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat gaaacgctgg
    84241 gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg ctcaactacc
    84301 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
    84361 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct gcacgatgat gtcgtgggcc
    84421 tgtgcttcca gggggcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg
    84481 atctgctgaa cctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc
    84541 tgctgaaata tgtgcacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc
    84601 tgcaacaggc ggtacagagc ggcgagtata gcgactatca ggaatacgcg aagctggtta
    84661 atgagcgtcc ggcaaccacg ctacgcgatc tgctggcaat tacgccgggt gaaaacgcgg
    84721 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga
    84781 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggctgaagcg atgaacagca
    84841 tcggcggcaa ctctaactcc ggtgaaggcg gcgaagatcc ggcgcgctac ggcactaaca
    84901 aagtgtcgcg catcaagcag gtggcttccg gtcgcttcgg tgttactccg gcgtatctgg
    84961 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaaaccgggt gaaggcggtc
    85021 agttaccggg cgataaagtc acgccttaca ttgccaaact gcgctattcg gtgcctggag
    85081 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc
    85141 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
    85201 aaccgggcgt tggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca
    85261 tcgcaggcta tgacggcggc accggcgcaa gtccgctttc atcggtgaaa tacgcaggct
    85321 gtccgtggga gctggggctt gttgaaaccc aacaggcgct ggttgccaat ggtctgcgtc
    85381 ataagatccg tttgcaggtt gatggcggcc tgaaaaccgg cgttgatatc atcaaagcgg
    85441 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc gatggtggcg ctcggctgta
    85501 aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact caggatgaca
    85561 aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac tttgagttta
    85621 tcgcacgtga aacccgcgag ctgatggcac agctgggggt aacgcgtctg gtggatctga
    85681 ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg
    85741 cgctgtcgaa gctgctggaa actgccgagc cacatgcagg taaggcactc tactgcaccg
    85801 aaaaaaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa caggcaaaac
    85861 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac accgaccgtt
    85921 ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag ggtctggcag
    85981 ccgatccaat caaagcgtac ttcaacggca ctgcaggcca gagcttcggc gtgtggaacg
    86041 cgggcggcgt ggaactgtac ctgaccggtg atgccaacga ctatgttggt aaaggcatgg
    86101 cgggtggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
    86161 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
    86221 gcgcgggtga acgtttcggc gtgcgtaact ccggtgctat caccgtggta gaaggcattg
    86281 gcgacaacgg ctgtgaatat atgacgggcg gtatcgtctg tattctgggt aaaaccggcg
    86341 ttaacttcgg cgcaggcatg actggcggct tcgcttacgt tctcgatgaa agcggcgatt
    86401 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
    86461 atgaagagca tctgcgtggt cttatcaccg agcatgttca gcataccggt tcccagcgtg
    86521 gtgaagagat tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga
    86581 agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca gagttgcgcg
    86641 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
    86701 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
    86761 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
    86821 acccatactg cgagtggaaa tgcccggtac acaactacat cccgaactgg ctgaagctcg
    86881 ccaacgaggg gcgtattttt gaagcggcgg aactgtcgca tcagaccaat accctgccgg
    86941 aagtgtgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc accctgaacg
    87001 atgagtttgg cgcagtgacc atcggcaaca ttgagcgcta tatcaacgat aaagcgttcg
    87061 agatgggctg gcgtccggat atgtccggcg tgaaacaaac cggtaaaaaa gtggcgatta
    87121 tcggcgctgg cccggcgggt ctggcgtgcg cggatgtcct gacgcgcaac ggcgtaaaag
    87181 cggtcgtctt cgaccgtcac ccggaaattg gcggattgct gaccttcggt attccggcct
    87241 tcaagttaga aaaagaggta atgacgcgcc gccgtgaaat cttcaccggt atgggtattg
    87301 aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgacctg ctgagtgatt
    87361 acgatgccgt gttccttggc gtcgggactt atcagtcaat gcgcggcggg ttggaaaacg
    87421 aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa
    87481 tgggctttgg tgaaaccaac gacgaaccgt tcgtcagcat ggaaggtaaa cgcgtggtag
    87541 tacttggcgg tggcgatacc gcgatggact gcgtgcgcac gtctgtacgc cagggtgcaa
    87601 agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccaggttcc cgccgcgaag
    87661 tgaaaaacgc gcgggaagaa ggtgtagagt tcaaattcaa catccagcca ctggggattg
    87721 aagtgaacgg taacggcaaa gtcagcggcg tgaaaatggt gcgcacagaa atgggcgaac
    87781 cggatgccaa aggccgtcgc cgcgcggaga tcgtggcagg gtcagaacat atcgttccgg
    87841 cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac
    87901 acagcgtcga gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct
    87961 tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg
    88021 tcgttaccgc cattgccgaa ggtcgcaaag cggcagacgg cattatgaac tggctggaag
    88081 tttaagcgag gtaacaataa attcgccaga cagtgtctgg cgaatttata aaacgagcta
    88141 tccaactaaa ttttaagcca ttaaatcagc cttaatcttc actaccacct ttttaatctc
    88201 accaggttcg ccaacaacgc atcccggttt atgcggttct cctggcataa acacggcgaa
    88261 cattcccggt ttcaggatga tggcttgctc gttttcaatg gcgctgcaaa gctgataatc
    88321 atcttcatgg tggaactctt cacactgacg cgcagtgcct gccatgccaa acagaatccg
    88381 ttcctcaccg tttaataaca gctggatatc aatgtattgt tcgtgcaatt cggctttttt
    88441 ctcaacgggc gattgagtat taaatgtcat gacattcata aagatattgt cgccctgtaa
    88501 ttcgtaacgg ccaggcgctt tttcttgcgg tctggcagct aatgccaacg ttaacgcgtc
    88561 ctgtaacgca ggatgtaatc cggcagacgg taatgactgt acttcaccca tcatcataat
    88621 atttctccct gggccaacag cgcagcccca agtaaacctg catcgtggcg gtagtgcgcc
    88681 gccagtaaat caacatgaaa tgccgctggc tcctgcgcca gatacgtttc caccagcgcc
    88741 agataccctt ctgccagacc aacgctgcca ccgaccacca cgcactggca atcagttgtg
    88801 gctttaatat cagcgatcag ccttgcaagc gtacgtgcgg agcggtgaat cagttgctgc
    88861 gcctgctcat caccctgccc ggcgcgcgtg aaaatagttt tcgcattcgc accagccaac
    88921 tccccctgcg ctgccgctgc aatgccgcga ccagaagcaa tcgcttccac gcaacctgtg
    88981 cgtccacagc cgcagactgg accatgcgga tcggcaagcg tatgcccgat atgccccgcc
    89041 agaccgccag ggccggtacg cagtttaccg ccgctcacta caccgccgcc aacgccggtg
    89101 gaaacagtga taaacaccat atcggttata tcgccatcca gcgcctgata ctccgcccat
    89161 gctgcggcct gcgcatcatt aatggcaatg gtcggcaaat tggtaagttg ctccagcgtt
    89221 ttgactaacg gaaagtgtaa caatccaccg agattatgcg gattcagcgc cagcaagctg
    89281 ccgtcgcgaa taattccggt tgaagcgatg gcaacccgct gcgcatgtgc ttgcaacgga
    89341 gagactaatg cggataaggc atcacgcagc gcctgcggag tctggctggc aggcgtagga
    89401 agttctcggc gatcgcggat ctgcccgtca gcgccaatca gcgcggcggc aagtttagta
    89461 ccgccgatat caatcgccag tgtggtcata gcaccgcctt tttcatcgct gtgttgtacc
    89521 actgacaaat gtgttcaaga cgcgtaatgg cagaaccgac cgtcaccgcc cacgcgccgt
    89581 ggcgcatcgc atccgccgcc tgagcaggcg tgttgtaacg cccttcggca atcacccggc
    89641 atccagcgtc actcaacgtt ttcaccagcg ccagatccgg ctcttctggc gtttcaggcg
    89701 tagtatagcc agaaagcgta gtgccaataa tttcggctcc cagcttttgg catgccaggc
    89761 cgtcttccgg cgttgagcag tcggtcatcg ccagtaaacc gtgatggtga atacgtgcca
    89821 gcagcgtttc aactggcacc ggacgcggtc ggtcggtgcc gtcaatcgcg ataatgtccg
    89881 cgcccgcctg cgccagcgca tcaacatctt caatataggc cgtgatgcgt accggagaat
    89941 cctccagatc gcgcttcaca atcccaataa tcggcacgct caccaccgca cgcgtggctt
    90001 gcagatttgc cacaccttca atacgaatgg cgaccgcgcc cgcctgctct gccgctaatg
    90061 ccatggcagc gacgatttcg ggtttatcga gcgggctgtc cggaaccggc tggcaggaga
    90121 caatcaggcc accgttagca gcgatttttt gatccagttg tgcaagtaac gacatacatc
    90181 ttcccttagc gaaaggcccg gtacagagac cgggcaacag gattaacttt tggttttgac
    90241 taaattgttt ttggcgctgc caaatggcac ggcaccgcta aatggtttgc cgtcgatagc
    90301 gtcatgagta cgcaatgctt ccgggcgcag ccaacgctga acacgagaag gcatatccag
    90361 accaatcagc aggatcacca caaatgtcag actgaacgag agcgatgcca gcgcagtacc
    90421 cagatccaga cgttgagcga tcaacgcgcc gatgattggg gccagtgcac cgcccaatgc
    90481 gccaacgttg taggtaaagc ccaggcccgc tgcacgctgg tcggtatcga aataaccgcc
    90541 aatcagtttc ggtaagatcc cggcgatccc ttgtccaagc atttgctgga agaacagtaa
    90601 cagaccgagc acccagacgt ttgcgccgcc aatcgcaaat accggaataa tcagcagctg
    90661 cgaggccagc agactacaaa cgtatgcttt gcgggttccc agccagtcac cgaggaagcc
    90721 cccgacgcag caccccaccg ccgcgccaaa gccactaaag aacagcacat tggcgacagt
    90781 atgcgggtca taagccagat cggttttcag atacgttggc agcagcgcct gaatcggcca
    90841 tgagtagagg aaagcgaaca acacgaccac catcagcatt acgcccgttg gccagcgttt
    90901 gcccgtactc tgcaccataa agctgataaa aattgcggcg cataacagcc caagaacagc
    90961 gacgatcgcg gcattttgca ggttaccggc gaagcagaac cacagcgcag tagccgccgc
    91021 cagtgtcatt acgatattgg caatgcgatg ttcaccacgg tagagaatat ccaccattgt
    91081 gcgtaccggt gctttacccc cgtgtttctc tttccagtct tccgcttccg ggatgttttt
    91141 acgcagccag agagcaaaga tgattggcaa aatgccgata aagaacagcg cacgccagcc
    91201 ccagaccgga accaccagac tatagacctg agcggcaacg acggccccca ctgagaagcc
    91261 tgaaatcaga aaaccactgg ctttgttacg cagatgcttt ggccagcttt caatgacata
    91321 ggtggcgctg gaaccgtatt cacccgccat ccccatgcca atgaccagac gagcgataaa
    91381 catggtgatg tagcctggcg caaagccgca ggccagcgtc ccggccgaga agagaacgat
    91441 gctggtgacc attgccagac gacgcccgta gcggtcaccc atagcgccga gcatcaggcc
    91501 gccgaaccag cgagagataa aggctgcgga gatcagactt gccgcctgca ccgtcgtcag
    91561 cccgaattca ccctgtactt cggtgagtac cagggcgatt aaaacgaaat caaaaccgtc
    91621 aagcagatat cccaaccagg ctgcggaaaa tgcccgccat tgtgcgcggt taagatggcg
    91681 ataccacggg atattctggg ttgtagtact cattgtgagt ctcccgcggt gggcgatgcc
    91741 cacacgcttt ggtatgaaaa ttgtagggta cagatgcgtt tatttcccct cacccggtag
    91801 gggcgagcga ggggaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    91861 cagttctggc agatattttt catctaccgg accaaacggt ttgcggcaca gcggcacaga
    91921 aacgacatcc atataatgca ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    91981 taaatcaatg actttgttgc actcagtttg cagtttctgc gcggtctgga tatcgccttc
    92041 tttcagcgcc ttcacgatcc cctgataacg ccagcccatg atgttgtaag tgctgccgat
    92101 accgccgtct gcgcccgcca gaagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    92161 caccagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaggtctg
    92221 tttcagcgca cctacaccag gcagcgtaac cagggtgttg atctgatcca gggtcagttt
    92281 taccccgctc agggctggaa tgttgtacac caccatcggc aacccatccg ccgaatcaat
    92341 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    92401 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    92461 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    92521 gatttccagt acctgttcgc gctcggaaag gctttgtaca aaggcttcgc ctgtcgaacc
    92581 acccacgtac aaaccgtcga tgccctgctg aatattgaac tgaaccaggc ggcgcagact
    92641 cgctttatcc agtgcttgtt gttggtcaaa aggagtcagg agtgcagcca ttacgccacg
    92701 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    92761 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    92821 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaggcg tgccaggtag
    92881 cagagacgct gttgagatgc gattgcaacg cgcgatcggc ttcgtcagga tcatgacggc
    92941 ggatcgcgtc aacgatcgca atatgctgtt gatagctaac gttgttatgc tcatgcagag
    93001 cctgatcggc cacggttgga cgtgcggcga ttagccagtc gagcagggca acgtggatcg
    93061 ccatgaagat tgggttacca gggatctccg ccagaacgcg gtggaaatca acgtctgagc
    93121 gaatgaatgc cgcgttgtta tccagcgact gactgttgat ttccagtgct tttgccagca
    93181 aatcgatttg ctcatcggtg gcgtgttcag ccgcatagcg caccaggctg gattcaaaga
    93241 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    93301 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    93361 cgttgtttat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcaccgaag
    93421 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcac
    93481 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    93541 tacgcgccag cgggcggctg cgcaagttgc gcccgatagt ggtagtagaa tcttcagctt
    93601 gcggatcaaa tgcgttcata ggactcattc aggaaaatgc gttagcggat aataacgacg
    93661 ctatcgtagc acagtatcaa agtcaggcga aaagatgaca gatagcaaaa caggccacat
    93721 gggcctgttt tcattttact tcacaacgcg taatgccggt cgaccaccgc gtggtggctg
    93781 cggaggttca tcgtcaggat gagtttcatc atcgtgatct ggcttgtcgc catcgataac
    93841 cgacataacg gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggcatcttc
    93901 atcgtaggca gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc
    93961 tagcacagca gccagcggca cagaaacctg acgcggaatg ccaccaaagc gcgcgttaaa
    94021 gcgcacctca tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac
    94081 gatttgcccg tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac
    94141 caccaggtgc ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata
    94201 gggacgacgt ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt
    94261 tcacgttctg cttcagttaa agaagcaagg aaagagtcac gttcaaagac gcgggtcata
    94321 tagcctttca gctctttcgc acccgggccg ctgaactcga tgcccagttg cggcagacgc
    94381 cacagcagag gagcaaggta gcaatcgacc aggctgaact catcgctcag gaagtacggc
    94441 ttctgaccga agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca
    94501 tcggcttcag aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg
    94561 atgcgatgca tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc
    94621 ggatgcggga aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc
    94681 tcacgatcca ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc
    94741 ggattgtcct tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg
    94801 cggacctgat ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg
    94861 ttggcagcga cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca
    94921 ccaggtggcc agtcagaagt tgtgttaccc agtaaggaac gactctcttt gttcgaaaat
    94981 caaacaaaaa atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga
    95041 ttttgtgacc tttgtggtga gtcgatgccg gaaatgggga aaaagagatg cgctttagtc
    95101 tgaaatagtt gacttagtcc cttattggcg atgtggtttt tgttttgcct gttgtcagac
    95161 ggcagcaaaa agcaactttc cattttttac gctgatgcag attttagcca taaaaaaacc
    95221 cgccgaagcg ggttttttcg caaattgttt tctgccggag cagaagccaa ttaacgtttg
    95281 gagaactgcg gacgacgacg tgctttacgc agaccgactt tcttacgttc aacctgacga
    95341 gcgtcacgag taacgaagcc agctttacgc agttcagaac gcagggactc gtcgtattcc
    95401 atcagagcgc gggtgatacc gtgacggatc gcaccagcct gaccagagat accaccacct
    95461 ttaacggtga tgtacaggtc cagtttctca accatgtcga ccagttccag cggctgacga
    95521 actaccatgc gagcagtttc acgaccgaag tactgttcca gagaacgttg gttgattacg
    95581 attttaccgt tgcccggttt gatgaaaacg cgagctgcgg aacttttgcg gcgaccagtg
    95641 ccgtagtatt gattttcagc cattgcctat aatcccgatt agatgtcaag aacttgcggt
    95701 tgctgtgccg cgtggttgtg ctcgttaccc gcgtaaactt tcagtttacg gaacatagca
    95761 cgacccagcg ggccttttgg caacatgcct ttaaccgcga tttcaatcac acgctcagga
    95821 cggcgagcaa tcatctcttc aaaggtcgct tgtttgatac caccgatgtg gccggtgtgg
    95881 tgatagtaca ctttgtcagt acgcttgttg ccggttacag caactttgtc agcgttcaga
    95941 acgatgatgt aatcaccggt atctacgtgc ggagtgtatt ccgctttgtg cttaccgcgc
    96001 aggcgacgag ccagttcagt agccagacgg cccagagttt taccggtcgc gtcaacaaca
    96061 taccagtcgc gttttacggt ttctggttta gctgtaaaag ttttcattaa aagcttaccc
    96121 aataaatagt tacacgttgg tgaacaccca aacgtcttca attgttgagg ttcacacgac
    96181 aaagtccggc aaacctaccc cttcgaatag cctatgccag cacacaaaaa gtttcgggaa
    96241 aaaaaacttt cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga
    96301 cccctttttg tgatttgtgg caggttttac cccgccaaat gctcgcgctt caaatactct
    96361 tcgctttgca tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgctcg
    96421 ccctgataaa tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg
    96481 taaaactcat ccaccagcgc aataaagcgt cgcgcttcgc tctccatcaa ccgcgtcata
    96541 actggtacat caaacaacat gaccgtgtga aagaagcgtg agagcgcaat atagtcatgc
    96601 tgactgcggg cgtcgacgca cagcgtagta aaagaaaccg ccagcgtctg gttctcgacg
    96661 cccattgtcg ccaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcgcc
    96721 cccgccagcg ccaaccatag tttatccatt tgcgcccggg tttcatcgtt gaggggcgaa
    96781 agccacagat gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc
    96841 attacatcac aatgctgttt aatagcatcg attgcaggca aaaaacgcgc acgttgcagg
    96901 ccatttcgat aaagttcgtc cggcggaata tttgacgtcg ctaccagcgt tattccgcga
    96961 gcgaacaggg ctttcatcag gccgccaagt agcatggcat cggtaatatc agaaacaaaa
    97021 aattcgtcaa aacagagcac gtcagtttct gctttaaagc gatcggcaat aatttccagc
    97081 ggatcggtct gcccctgcaa agcagttagc tcttcatgta cccgcagcat aaaacggtga
    97141 aagtgcagac gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag
    97201 gttttcccgc gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg
    97261 tcttcgcgtt taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct
    97321 ggaggcgtgc tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt
    97381 tgaacgtcgt cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg
    97441 gtaatgcttt gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg
    97501 ttgacgaaaa aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg
    97561 aattaacggt tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac
    97621 ggaaacaaaa gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc
    97681 gtcggtatca ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa
    97741 caggcgttgc agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag
    97801 ctggttagcc actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc
    97861 cagctgtatc agcacatggc gaaaagctcc agtagcctgc tgccggaact gtctgcggaa
    97921 gcaaacccgt tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg
    97981 cagatgcctc gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc
    98041 gactaattta tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat
    98101 catttaatct ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg
    98161 aatctctttt cttatcattc aggtacgaga gcaggaataa tgaaaaaaca aacccagctg
    98221 ttgagtgcat tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg
    98281 tcgattccag gccaggttgc cgatcaggcc cccctcccca gtctggctcc aatgctggaa
    98341 aaagtgcttc cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa
    98401 atcccggaag aattcaaaaa gttttttggt gatgatttac cggatcaacc tgcacaaccc
    98461 ttcgaaggtt taggctccgg tgtcatcatc aacgccaata aaggctatgt gctgaccaac
    98521 aaccatgtga ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt
    98581 gatgcaaaac tgattggtag cgatgaccag agcgatatcg ccctgttgca aattcaaaac
    98641 ccgagcaaat taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt
    98701 gccgtggcgg tcggtaaccc atttggcctt gggcaaaccg ctacctccgg gattatatcc
    98761 gcattaggcc gcagcggtct gaatctcgaa ggtctggaaa actttattca aactgatgcc
    98821 tccattaacc gcggcaattc cggtggtgcg ctgttaaacc ttaacggtga gttaattggc
    98881 atcaacactg caatccttgc gcctggcggc ggtagcgtcg ggattggatt tgccattccc
    98941 agtaatatgg cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt
    99001 ctgttaggca tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac
    99061 gtgcagcgtg gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc gaaagcgggc
    99121 gtcaaagcgg gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag
    99181 ttgcgctctc gtatcgcgac caccgagccg ggcacaaaag tgaagcttgg cctgctgcgt
    99241 aacggcaaac cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc
    99301 gctgaaatga tcacgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat
    99361 ggtggcaaag gtattaagat cgatgaggtt gtcaaaggaa gcccagctgc tcaggctggc
    99421 ttgcaaaaag acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa
    99481 atgcgtaaag tgctggcggc aaaaccggcc ataatcgcct tgcaaatcat acgcggcaat
    99541 gaaagcctct acctactaat gcgttaatgt cgtaaaccgg gcatcaggct gacgtgtgat
    99601 gtccggttaa ctcgtggtat gctgctgccg ttcccttttt taacgatgcc tccatcatgt
    99661 ttgtgaagct cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg
    99721 ccatgccttc gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg
    99781 agacgcctgc cagctataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt
    99841 ataaccgcgg tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatctg
    99901 gtgtaatcat ggatcaacgc gggtatatca tcaccaataa acacgtgatc aacgacgccg
    99961 atcagatcat cgtcgcctta caggatggac gtgtttttga agcgttactg gttggctctg
   100021 actctctaac cgatctggcg gtgctgaaaa ttaacgccac aggcggttta ccaacgattc
   100081 cgatcaatcc acgccgcgta ccgcacattg gcgatgtggt tctggcgatc ggtaacccgt
   100141 acaacctcgg gcagaccatt acccagggga ttatcagtgc cacgggtcga atcggtctga
   100201 acccgaccgg gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg
   100261 gcggcgcgct ggtgaactcg ctgggcgaac tgatgggcat taacacgctg tcgtttgata
   100321 agagtaacga tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcga
   100381 ccaaaattat ggataaactg atccgtgatg gccgtgtgat ccgtggctac attggcattg
   100441 gtggacgtga gatcgcccca ttacatgcgc agagtggcgg catggatcaa ctgcaaggga
   100501 tcgtggttaa cgaagtgtcg cctgatggcc cagcggcaaa tgcaggcatc caggtcaacg
   100561 atctgattat ctctgtgaat aacaaacccg ctatttccgc cctggagaca atggatcagg
   100621 tcgcggaaat tcgccctggt tcggtgatcc cggttgtcgt gatgcgtgac gataaacagt
   100681 taatattgca gctcaccatt caggaatatc cggcgaccaa ctaaggtcag cgtaaaaaaa
   100741 caaaaaccgg agtctgtgct ccggtttttt attatccgct aatcaattac ttattaacga
   100801 actcttcgcc cagggcgata tctttcttca gcgtatccag cataccttcc agcgcgttct
   100861 gttcaaatgc gctcagagta ccgatagatt tacgctcttc cacgccgttt ttacccagca
   100921 gcagcggttg agagaagaaa cgagcgtact gaccgtcgcc ttcaacgtag gcacattcga
   100981 caacgccttg ttcgccctgc agtgcgcgaa ccagagacag accaaaacgt gcagctgcct
   101041 ggcccataga cagggttgca gacccgccac cggctttcgc ttcaaccacc tcagtacccg
   101101 cgttctggat acgtttggtc agatcagcca cttcctgctc ggtaaaacta acgccaggaa
   101161 cctgtgacag cagcggcaga atggtaacac cagagtgacc accaataacc ggcacttcaa
   101221 cttcgcctgg ctgtttgcct ttcagttccg caacaaaggt gttggaacga atgatatcca
   101281 gcgtggtaac gccgaacagt ttgtttttgt cataaacacc ggcttttttc agcacttcag
   101341 cagcaatcgc aactgtagtg ttaaccgggt tagtgataat accaatgcac gctttcgggc
   101401 aggttttcgc aacttgctgt accaggtttt tcacgatgcc ggcgttaacg ttaaacaggt
   101461 cggaacgatc catacccggt ttacgcgcta cgcctgcaga gataagaacg acatctgcac
   101521 cttccagcgc cggagtcgca tcttcacctg aaaaaccttt gattttcaca gcagtaggga
   101581 tatggctcag atcgacagcc acaccgggag tcactggagc gatatcatac agagagagtt
   101641 ctgaacctga aggcagttgg gtttttaaca gtagtgcaag cgcctggcca ataccgccag
   101701 cagcgccgag gactgcgact ttcatcctaa actccttatt atattgataa actaagatat
   101761 gttgctccgc tgccgcgacc ttaatccaca aaattgccgt ttacaatgac cacatctcaa
   101821 gaatgtgtag tcacgcaagt ttagcgttta tgcatttaat tgccgtaatc aggaacctaa
   101881 cttacgtaat taacagtcac ttatgcatta gcgcaacatt ccaacaggtg gtgacaatat
   101941 accctaccgc tcagccaaaa caatatcaat ttgataacaa ttaatttact tttaagcaga
   102001 atttgcaggc cgtgacgcag gcatgtttct caataacgaa atttgataaa atcccgctct
   102061 ttcataacat tatttcagcc ttcttcaggg ctgactgttt gcataaaaat tcatctgtat
   102121 gcacaataat gttgtatcaa ccaccatatc gggtgactta tgcgaagctc ggctaagcaa
   102181 gaagaactag ttaaagcatt taaagcatta cttaaagaag agaaatttag ctcccagggc
   102241 gaaatcgtcg ccgcgttgca ggagcaaggc tttgacaata ttaatcagtc taaagtctcg
   102301 cggatgttga ccaagtttgg tgctgtacgt acacgcaatg ccaaaatgga aatggtttac
   102361 tgcctgccag ctgaactggg tgtaccaacc acctccagtc cattgaagaa tctggtactg
   102421 gatatcgact acaacgatgc agttgtcgtg attcatacca gccctggtgc ggcgcagtta
   102481 attgctcgcc tgctggactc actgggcaaa gcagaaggta ttctgggcac catcgctggc
   102541 gatgacacca tctttactac ccctgctaac ggtttcaccg tcaaagagct gtacgaagcg
   102601 attttagagc tgttcgacca ggagctttaa tctctgcccc gtcgcttctg gcggcgggga
   102661 aaatgttgct tttgcctatc aaccccctgc tttcccctgc gattcattta acgaatagtg
   102721 cgttttactg cgacatatca ttcacacaat gaatacatat agtaaaaaag cacattatgc
   102781 aaaattcatt atctaattga aaatactata attaacaata aatgatcgtg tttttaattc
   102841 ttttttgtta ttaaaattca catttttaac acttagtatc aactgaaaca gttagcgtgg
   102901 tattaattag ttcaataatt agtgtatact tgattttgtg atatgggtca cgaaacaaag
   102961 gcccagctaa aagattatgt cgaggtaaaa atcatgaaaa tcaaaaccac tgttgctgca
   103021 ttaagtgtgc tttctgttct ctctttcggt gcattcgctg ccgactccat tgatgctgca
   103081 caagcacaaa atcgtgaagc aatcgggacc gtatccgtaa gtggtgtggc gtcttcgcca
   103141 atggatatgc gtgaaatgct gaacaaaaaa gcggaagaga aaggcgcaac ggcctaccag
   103201 attactgaag ctcgtagtgg tgacacctgg cacgctacgg ctgaactgta caaataaacc
   103261 ctcatcgtct tgtccgacga tattgccccc ggttcggggg ctttttttgc gctaatgacg
   103321 aacattaaaa cgcaaatgcc cttccagctc ttcctctgcc tcatcaaaca gcaatattaa
   103381 cgcgccaaaa cggcgacgcg ttttctctcc cagatgaaca aattcaatct caagtggtag
   103441 cggcaggaca tcgttcatta acacatccca tagtgagtcg agatcgcgta ccttatcttt
   103501 cgccagacca aaggtttggc taaagtcacg ataaaaatcc tcctgactct caatctcatc
   103561 aaaatcaaag gtataaatat tcatctgttg ccaccgtcac gtttcgcggc gagttctgcc
   103621 gttcatcgct aagtatagcc atgaaaaaac cgacgcttcg ggcgccggtt ttgacttaac
   103681 tatcggtcaa cgcatgttga tacttatgga gcatacccgt aagccgatgt actggttccg
   103741 tcacctgcgg tggcgcttgc cagatgcgta gcttttcctg gtagatttcc agttcttcca
   103801 gcaactggcc aaagtaccga cgacgtttat catcgctacg ggcagatatc acatggtctg
   103861 ctgtgcggcg catttgccgg tgaaacgccg ataaatcctc gttaaccgga atcggcgcat
   103921 cacgcaggcg ctggtgcgcg ataatcatcg tcagcgccag gcgaaatttc ggcaaatccc
   103981 ccgggaactt attcatcagc aaaaacagct gctgataaag tgccgggagg tgattctctt
   104041 tacgacgtgc cacattggta gtcatcgcgg aaacagcggc agaaacaaac tgattaagca
   104101 gtacacgtcc agtcctgtcg cgcgatttat cccgcaccag caaaataacg gtgaacgcga
   104161 gcacacagcc gacgatttgc cctaatgcgc tgtcgagaaa ctgactgaaa tggaaagtca
   104221 tcgggttatc cagcacgata atgtttatgg tgctggccaa cgcccccatc gagcccaatc
   104281 tccgtttctg tacttctata ccgaggaaga atcccagcac tgccaggcta atacacagca
   104341 gcaacatgct ctgttgggta ttagggataa tcaccaaaaa gtagagcagc cctaacggta
   104401 gcgctgccag tgtcccgtag ataaagtcga tcgccaccat gcgtggattc ggcaaacgca
   104461 ttgccagtga cgtcactacc gcaatcatca ccattgcacc actgccggaa gtccagcccg
   104521 tccacagcca gaaaagcgtg cccagaatgc aagaaagtgt ggttcgccag aagttaacca
   104581 ttgcatgatg acgttcggct gactctactt ttacttccgg ttcgccttgc aggatctctt
   104641 cttcggtggc gttgattttt gtgttactga taacgccgcg cttgagaagc tgataacgcg
   104701 ttgccgccgc gacccagcta taaatggtga caggcgtttc ccgttccccg gtccaggcga
   104761 tcactctccg caggcgtttg agctgcttgt ggacgtcctg cgcagtttct accggcgtgt
   104821 caaaaaattc gcggaaagta tcagtgatca attccgggcg cgtattctga ataagataag
   104881 tttcgcagga ttgggtaatc agcgtcagcg atagcgtatt gatcgctttt aaacgtcgat
   104941 tggcccgcgc ccagcgggaa gattccatat tcaggttgct gcgcatacct tgtaacgccg
   105001 tagtacgtcg caccagatcg ccccaggctt tatcgacaac ttcaccatcg ccatgcttga
   105061 tacagagttg cattaattga tattgcgcaa ccagcaaact ttccagctct cgatccactt
   105121 cttgtttgat cgatcgcgga gaaaagagca aatccgccat aattgcacac acaataccga
   105181 taacgatctc gctacaacgt tcgacggcaa actgcggcgt aagcaatggt tccggctgaa
   105241 tggtgatcac aatgatcagc gcggtataac cggccagccc ccacgcatac gagttttcta
   105301 ttcgtaccag cgaggatatc caggtacaaa aaccggccca gatacagcac accagaatca
   105361 tcaataatgg tgctcggatc atcgcaatga tgattaccag tccggcaata cagccaataa
   105421 atgtgccgat gatgcgcaaa aagccacgat agcgaatggc gccagaatac ggttcacctc
   105481 ccgcagcaaa ggccggaccg gcggcaacaa tcgccgctgt cagtaccgcc cagcgtggcg
   105541 tttccagctg gaagtgaaag ccaacaaaca gcgccagtac aatggcggtc gccagtttta
   105601 ccgcaaagcg aatatgttga ttagcaatgg agaaaatacc catcgtgatt aaccaaactc
   105661 acgcaggcga tgagccattt tacggaagaa cgaatcctga ctttcgtcgc gatcttgttt
   105721 gccagtgact accaccgtag cagtggtgcc cgcaggccag atgttctctt gctggttgtc
   105781 gagacgaata cgtaccggaa cacgttgcgc caaacgtacc cattcaaggt tagagtcgat
   105841 ggtcgccatc cctttgtcgt cacgcgtgct gctggcattg gtgacccctg cggcaacact
   105901 atcaacagtc cctttcagca ctttgttact gccaagcggc gtgatctccg cacgataccc
   105961 agggcgcacc ccttccagct tagtttcttc catataggcc agtacataga aggagttctg
   106021 tttcaccagc gcaaccgccg ttgatcctcg agtaataaac tcaccggtat agacgttgag
   106081 gttggtcacc cagccatctg ctggcgcgcg gatgactgta cgttcaagat ccagttttgc
   106141 cagatcgcgg gtcgcctgcg ctttcgctaa ctgatgcaga actgtttgta gtacgttgtt
   106201 ggcctggtcg atctcttcgc gagacatcgc ctgcacaccg agacggttac gacgtccggc
   106261 ctcctggcgt ttctcctgcg ccagtacctg ataataagca acatcggctt gcgcttcctc
   106321 aagcgccttt tgataacgcg gctggtcgat ggtgaacagt acctgtcctt ttttcaccag
   106381 ctggttatca tgaacattca cctgggtaat gagtccagaa acgtccggcg cgatcgcaac
   106441 gacgtcagcg ctaaagcgcg cgtcacgcgt ccacggggat tcggtgtaat agacccaggc
   106501 attaaaaatt gcgatgaagg ccagaatgac taatacgacc gtgatggccg tacgggagaa
   106561 ttttcttatt agtgttttca cttcaacctc aaacgaacag tcgcgatatc aaataaaaca
   106621 agcagcaata gagcgcggtg ttgaacaacg ccggatgcca gacaaagtcg taaatacctg
   106681 ttggcacaag tacccggcgc accagccaga aaatcgccag tgataaaagc aattcgaaaa
   106741 atatcggtgg gaaggacagc ccaaacacca cgataacggg aaacagactc atgttgacct
   106801 tggttgtata gagagagcag gcgttattat tttcagcatc tgtcgccgca gagaagggca
   106861 tggaaagccg ggcgagagca acattgctgt agattgatat ttaatatatt aacgtaactg
   106921 ttatgctgtt atctatatta tgtgatctaa atcactttta agtcagagtg aataatggaa
   106981 cgactaaaac gcatgtcggt gtttgccaaa gtagttgaat ttggctcttt taccgccgcc
   107041 gccagacagc tacagatgag cgtttcgtcc atcagtcaga cggtgtcaaa actggaagat
   107101 gagttgcagg taaagctgtt aaaccgtagc acacgcagca ttggcctgac cgaagccggt
   107161 agaatttact accagggctg ccgtcggatg ctccatgaag tgcaggatgt tcatgagcaa
   107221 ctgtatgcct tcaataacac ccccatcggg acgctgcgca ttggctgttc ttcaactatg
   107281 gcacaaaatg ttctcgccgg gctgacagca aaaatgctga aagaataccc aggtttgagc
   107341 gtcaatctgg ttaccggaat tcctgccccc gacctgattg ccgacggtct ggatgtggtg
   107401 atccgcgtcg gcgcgttgca agattccagc ctgttttccc gccgtctggg cgcgatgcca
   107461 atggtggtgt gcgccgcaaa aagctatctc acacaatacg gcataccaga aaaacccgcc
   107521 gatttaagta gtcattcatg gcttgaatac agcgtgcgac ccgacaatga atttgaactg
   107581 atcgcaccgg aagggatctc gactcgcctg atcccgcaag ggagatttgt gactaatgat
   107641 ccgatgacgc tggtgcgctg gctgactgca ggcgctggga tcgcctacgt gccgttgatg
   107701 tgggtgatca acgagatcaa tcgcggggaa ctggagatcc tgctgccacg ttaccagtca
   107761 gacccacgcc cggtttatgc gttatatacc gaaaaagata agctgccgct gaaggtacaa
   107821 gtcgtgatca actcgctaac ggattatttt gttgaggtcg gtaaattgtt tcaggagatg
   107881 cacgggcgcg ggaaagagaa gtaattaata ttgtactgtt acgttgtaca aacctgtgcc
   107941 aacgggttcc cctcacccta accccagaag ggcgagggga ccgttcgtac acggagaagg
   108001 attaattatc cttctgaaaa tactgaaatt attacgcagt accgccaaca gtcaggttat
   108061 caactttcag cgttggctgg cccacgccaa ccggcaaact ttgcccttct ttaccgcaga
   108121 cacccacgcc gttatccagt ttcaggtcgt tgccaaccat cgaaatctgc tgcatggttt
   108181 cgataccgga accaatcaac gttgcgcctt tcaccggctt agttacttta ccgttttcaa
   108241 taagatacgc ttctgaggtg gagaaaacga atttgccgga ggtgatatcc acctggccgc
   108301 caccaaagtt cggtgcatag ataccgtact caacggattc aataatttcc tgcggggtcg
   108361 atttacccgg cagcatatag gtgttggtca tacgcggcat gggcagatga gcgtaggatt
   108421 cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg cgcgttgagt ttatcctgca
   108481 tgtagccttt cagaatgccg ttctcaatca gcacgttgta ctggcctggc gtaccttcgt
   108541 catcaatcgc caccgaacca cggcgatcga ccatcgtgcc atcatcaacc acggtacaca
   108601 gttctgaagc caccaactcc ccgacctgtc cactaaatac tgaagtgcca cggcggttga
   108661 agtcgccttc cagaccgtga cccaccgctt catgcaacag cacgcccggc caacctgcgc
   108721 caagtactac cggcatggtg cccgctggtg cagcaacggc agaaagattg accagcgcca
   108781 tacgcactgc ttcttttgcc cacgcatccg cacggacttc gccgtcgaga tcagcaagga
   108841 agaactcata accaaaacga ccgccgccac cactggcacc gcgttcgcgt ttgccatctt
   108901 cttcgaccaa aacgctcacg gaaagacgca ccagcggacg gacatccgcc gctagtgtgc
   108961 cgtcggtggc cgcaaccaga attaattcat agacaccact gaggctggca gtcacttcct
   109021 gtacgcgctt gtccgcttcg cgggcaacct tatcgacgcg acgcagaata tccaacttct
   109081 cttcacggct catgctttgc agcggatcta ccgaggtata taacgggcta tgctctaccg
   109141 cgcccagcgt ctgtactttg ccatcaccac tatcacggac gatggtgcgc gccgcttgtg
   109201 cactctgttc cagcgccagc aggctgattt ggtcagcata agcaaatccg gttttttcac
   109261 cactgattgc acgcacgcca acgccctgat cgatgttgta agaaccatct ttaataatac
   109321 ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa atagagatcg ccataatcaa
   109381 ggcgacgttc ggccagttga ccgaggatcg cgaacaagtc ctgatgtttc aggccgttcg
   109441 ccgctagcaa ttgttcactt accaggttca gactcatcgt ttttgctact cgttagttac
   109501 tgcagtagag gattttttac ggctgccgga tgcggcgtga acgccctgtc cggcctacgg
   109561 ttatgttctg gctcgttggc ctttggcaac gattatcata tgagattggg gcaattacgc
   109621 gccctcgtca aatcattgcg ctttttcttt acgcggttgt cgcaacactt cgttgatttg
   109681 cggatcgtcc agcggacccg aaatgtgata gcgcaaaatg gagactttgc tccacagcgg
   109741 acccagcact ttactggcgg caaacactgc cgcgccaaca atggggttaa ccgcaaaggc
   109801 cgcagccaca cccaccgtcg cagaaatctc tggtgcgaca accgcttcca tattcaggtc
   109861 gcgacgtacc agatttaccg accctttcat agcgatgtcc gcttccaggc catccaccag
   109921 cgtgtcgtcg gtgtgcataa cgccgtcttt aatccacgcg gtgctgcgaa tggagtcaaa
   109981 atagaaccct tcgccaaaag tgtctctgaa atcaaaacgc agcttacgca tcagggcatc
   110041 tacgctcaat aagcgcagca attgcccggc atgtccggta ttgatttcgg taatttcgcc
   110101 tttacccagt tgagtatgaa tgatgccatt caacgtcgcc tcatctggct gccagggtgc
   110161 tttgcgccag tgtaaatcgt aatccacatt aaatgacgac tggcgtacgg gcgtcgtgac
   110221 accaaaaaat tctgcggcgg catcaatttt ctgcccgcgc agttttcctt tcagcgaggt
   110281 acgttcattc cccggattgt taacccattc accatcggca gtgagccgcg aaaaaccagt
   110341 atcaatcagt ccattagtca gcgttaacgt atcgccagaa atggtgatat cactgtcaat
   110401 acgaccgaat ttttgccccc agaaccagca ctctgcgcat cgtatttgtg cgtctggcca
   110461 gccacggaag ttaatgcgtt ccgttgtcgg gaaaggtgat gactgtgttg aatcaccacg
   110521 agttttcgcc acgcttgggt tgtaataaag atatttgata ttcgccagcc acggtgcgtt
   110581 attacgcatc gccagcgtgg cgttgatttc acgcccttgc gcctcaacca gggtgccatt
   110641 tgccgtcggt tgcgaaacaa tactcaggtt attccattgc tgatttccca gcgacaacat
   110701 aggcgtacgt aacgttatgt gttgtgggaa actcgctgca ccaccgacac tctccgccgc
   110761 gcctttctgg aacagggcca gccactcggc accattcatc ggcggcatat tgagttcaac
   110821 gccactttgt tccggcaacg gcgggagcgt tttactgtct gccgcccaaa tagcacgatc
   110881 gagcgtcagc ttttgaccga gcaaccagcg gctattgaaa tggttatccg caccagcctg
   110941 tccggttaat tcaaagctgt tgagattgcc atcaacctta acgtttaccg gtagtggttc
   111001 acccgcaggt ttggctaacg gtgaaggtaa gtgactgctc acattcttca aatcgccgtt
   111061 cagctctacg ttatacgtcg cgccagcatg ataaggcagc tcaatgccca ctttaccatc
   111121 ccacgccacg ctgccactta atgcttcgtt caccgctgca ggcagaaagc cgattttcgc
   111181 tggttgccag ttaccgttga gattcaccgc gacctggtag gcttttgccc cttctttggt
   111241 ggaaaaatcc acgttcaacg gctgattaaa ccagcttgct gtcagtggtt cactttgcag
   111301 atcgccattg ataaagctga atttaccgct caaatttttc agggtgctgt cgagtggctt
   111361 gataaacaga ctgttattac gcagcgtcac ttcccctttc gcggttacca actcgccgtt
   111421 cagcgggata tcaagatgta agcgagcatt cacatcgccg tcgagcttga gttcttgcag
   111481 ggtcgcaccc agggaatctt tcagcggtgt ctcatcaaag taagggccaa cggctttacc
   111541 cggaccttta atgtcagcgt caatcagcag tttttctttt gagtagtcag ggatcactgc
   111601 ggtgagatta ctcgcgcgca cgccgcccag attaacgcca tcggttttca tccataaacc
   111661 gtcgttaata aagtccagtt caatatcaag gttagttaat gcaggccagt ccggctggaa
   111721 ggcaaacttc gcgttgcgca gcggcaccag cacttcaaac tgaccttcgt tgtgtttata
   111781 ggggaagagt tgcggattgc caccataaac cagcgtcgcg ttatccgctt caccgccctg
   111841 aatcgcgcca cttaagtaat caaccaggtc tttacccatc aagttttccg ggaaatagcg
   111901 ccaggcttgt gaaccatcat cggtactgat gccagccaga atacccagcc agggttcatc
   111961 gttagcaggt tgcaggtaac gaaaaccgcc gcgcgcatgg acggctttgg ctttaacgtc
   112021 aatattacgc ccatccagct ggaaaccttt gtcattgttc agccagctta tcgttgcctg
   112081 gccgtcggcg atttccagtg gcgcacggaa taccgtttcg taaggcatct ttgcctgctt
   112141 catcgacgca gtaagcaaac cattttctac gctgccggaa agcgtcccgg agaagtgttc
   112201 cgcacccggt aataatttcc attgcttcca ggccagatcg ctccacgatg cctgaaaacg
   112261 ggtcttgtct gccgccagaa gcgggatatc cagcgccaga gtgttaatct tgccgctcgg
   112321 ttgtgtggag cgccaaacat cacccagtgc aggtgaaagt ttcgcgacca gcgggcgtac
   112381 gccctccagg cctgccagct ccagattact ggcgcgaatc cggagttcgt cactgcgttt
   112441 attgtctttg ccgccaacgt cctgttccgg tatccaggcc agcgtcaatg ctccgctcgg
   112501 ccagggtttg ccgtccatcg tgatccgtgt atcgggaata gagaactgcc atcccggatt
   112561 ttcacgcgta atatgcgcag tcagattatc caccgacagc gtatgtgttt ccttctcgcc
   112621 caaccagctg gcaccgccct gtttcagcca gacgtcaccg ccggttacat cgcctttgtc
   112681 gatcgtcatc cagccttcaa gggagaactg tgccgtttcc agcgcaatat tgtcctgcat
   112741 ccatttaccg agccacggct tcaggtcgat gtcatccgcc tggagccaga cgcgaccatt
   112801 gcttaacaac ccctcatcat cgcgcaaatc catacgcacc tgcatcacgc cgtgctgtcc
   112861 ggtaaggctg gagaggctta ccaggccttc cgcacggtgt cgacgtggat cgttcagcca
   112921 ggtgagctgt gggatcgcca gctcggcgcg ctgaccggat ggcgtcagga aactgacttc
   112981 actgtcgcgg agatcgaaat ggtcaaattg acgaagaaac agatcgctga tgtgactggc
   113041 ttccaggcta tcattaccac cgccgctggt gataggagtg ttggtgcgaa agcgcagctg
   113101 ccagaaagtg aggtcgcgaa actgccagcg catatgtaac aggctctgcc agacatccag
   113161 cgcaagagta acgcgtttaa ccgaaaattc gccgccatct tttagttctg cacggatgtc
   113221 gtgcgcttca agcgtcggac caaaattctg ccagctggct gagagctgac tggcttctac
   113281 cggcatgcca gtcgcggatt ctattttgtt gaggatttcc ggacgccagg cgtcaagatg
   113341 cggtaaagca atacgcaggc cgctaaccag cagggcagcg atcacaacga gcgcggctcc
   113401 agtaagcagt aaaatccccg gcaatcgcct cacccgtcac tccttgtctg ctaaaaatgt
   113461 gactcaaaaa ccctttgccg gatggcggcc cagcatctgt ttacatcatt acgacgtcaa
   113521 actgctcctg gttatagagc ggttcaattt gtactttaac ctgtttgcca acgaaaattt
   113581 ccacttccgc cagcgagtgt gactcttcgc ctttcaaggc ttcggctact gccggagaag
   113641 catagaccag gaaacggtcg gagtcgtaag catggtggac acgaacaatc tcgcgcatga
   113701 tttcatagca taccgtttcc acggttttca ccgttccgcg accgtggcag gttgggcatt
   113761 cgttacacag tacgtgctca atgctttcgc gggtgcgttt acgcgtcatc tccaccagcc
   113821 ccagcgccga aaaaccatta acgctggttt tcacccgatc tttgctcagc gcctgctcca
   113881 gcgagtgcag cacgcggcga cggtgatctt cattattcat atcgatgaaa tcaataatga
   113941 taatcccgcc cagattgcgc aaccgtaact ggcgagcgat agcctgcgtc gcttcaatat
   114001 tggtattgaa aatggtgtcg tccagattgc gatgaccgac aaacgctccg gtattgatgt
   114061 ccacggtggt catcgcttcg gtctggtcga taatcagata accaccggat ttcagttcta
   114121 ctttgcgttc cagcgctcgc tgaatttcgt tttcgacatc aaagagatcg aaaatcggct
   114181 ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg aatgtactcc gaggtaaact
   114241 ccagcaatgc ttcgtaagtc aggcgtgagt caacgcgaat gcggtcgagt tcggcgtcgg
   114301 cgaaatcacg cagaacacgc tgcgccagcg ccagttctcc gtacagctga taacgggtct
   114361 gcgggcgttt tttacgctcc ataactttgg tccagacgcg tttcagataa gcggcatcgg
   114421 aggccagttc agcctcgcca accccttccg ctgcagtacg gatgataaac ccgccctgct
   114481 cgtcgcaata ctctgcgacc acttttttca ggcgttcacg ttctgattcg ctttcaattc
   114541 gttgggatac cccaacgtga gaagcccctg gcataaacac cagatagcga gaaggcagcg
   114601 tgatatcggt ggtcaggcgc gcacctttag tgccaagcgg atctttcacc acctgcacca
   114661 tcagatcttg cccctgacgg accagttccg agatgtcgcg caccgtgaat tgcttttgtt
   114721 cttcacccgc cacacattca gtgtgcggca tgatgtcaga tgcatgaaga aacgcggctt
   114781 tatccagccc aatatctaca aaagccgcct gcatacccgg aagtacacga cttacacgac
   114841 ccttgtagat attgcctact attccgcgtc gcgcctcacg ttcaatatga atttcctgca
   114901 gaataccgcc atcaatatac gccactcgag tttccgaagg cgttacgttt accaacaatt
   114961 cagccgtcat gtttatctct tttctcacgc agtgcgttaa aattacttaa taattcatac
   115021 gtttcaacca gcggtaagcc gactacggcg tgaaagctgc catttatctt cctgacaaaa
   115081 cagccaccca gcccctgaat accgtatgca cctgctttat ctaacggttc gccgctggcg
   115141 acatagcccg cgatgtcttc gtctgttaac gttctgaaag tcacatcggt gaccaccagg
   115201 caatcgagaa tgtgctggct gtcagccaac gccactgccg tcatcacctg atgggtctga
   115261 cccgataatt tgcgcaacat ctgcgccgca tgctctgcgt cgcgcggttt ctcgagcact
   115321 tctccgttca ggataacgat agtatccgca cccagcaccg ggagatcctg ctccgtttgc
   115381 gcgacacccg cctgtgcttt ctcgcgcgcc agacgcacaa catactgctg cgcgctctcc
   115441 tgcggctgac gctgctcctc aatgcccgta acaatacgtt caaaggtcac gccaagttgc
   115501 gcaagtaact cctgacgacg cggagaaccg gaagctaaat acagagaagt catagaaacc
   115561 tttattgcac tgcaaactgc tgacggactt tacgcatcag caagaaaatc cacggccaga
   115621 gcaccccatt gactacacta ctccagaaca cttccggtct gaaagagacg ttaatcacta
   115681 aaaactctgc ccagaaaaca ataatatcca ccaccagcga aagcaacatg acgaccagcg
   115741 cctgctgcca taatgcgagg ttgcggaaaa gctggtattt cagcgccacc aggtaagcaa
   115801 tgatgctcat cgccaatacg cgtacgccaa gcgtcgagcc gctgatcaga tccagtatgg
   115861 cacccatcac aaaacctgtg cccacattta cgcgatgggg caaggccagg atccaataca
   115921 acaagatgag taacacccag tttggccgga aaacaatcag attatccggc cagggcatga
   115981 tttgcagcaa cagcgcaatg aggaaagaga gccagattac ccagcgtccc tggctacgat
   116041 agctcgccac tattatcctc ccggcgcacg cgcaggcggt tgagcaccac tttgcggcgg
   116101 cgtagccctt tgtggagagc gattagctgc gggctgtgtg ggcgcagcag gcgcagtaac
   116161 tgcatttcct gtcgccggtt gctgcggagt cggctgagtg atccccgttg ccggttcagg
   116221 taactttggc cccatcgcgt ctggcgaagg caatacctgc ggcatcatct gcatcaaacg
   116281 ttcattagca acacgatgca cctcttccgg cgtcatcggg ttagcgccgt tacgatccgc
   116341 gccccacagc agcagcagat aacgcaaacg ttgcagtcct gcagtcggac gcgcctgaat
   116401 cacagtataa gcgcgctggg tatcgagttt tacggaagag acaaccgcga ccggatagcc
   116461 ttccgggaaa cgaccgccca gaccggaagt caccagcaca tcaccaacac gaatatccgt
   116521 attcgctggc agatgctcaa gctgcaaatc atccgtacaa ccgttaccgg ctgcaattac
   116581 gcggatatcg ttgcgcagca cctggattgg cagcgcgtgg gtcgcatcac aaatcagcag
   116641 cacgcggctg gtcagtttag cgacggccac cacctgacca acaacacctt tgtcgctgat
   116701 aaccggctgg ccttcataaa cgccattaac gctaccttta tcgataacaa cttgatcgct
   116761 ataaggatcg ttaaccgtgg agataacctg agtcaccatt ttctgctcat cctgacgcag
   116821 cggggaaccc agtagctcgc gcagacgcgc gttctcctgt ttgtattgtc caagcatcag
   116881 cagttcactg tttttcagca acagttcctg acgtaacgcc cggttttcaa gttctagttg
   116941 gtcacgcgag gccagcgtct gcgatacgcc atccagcaat tcacgaggag cattggaaac
   117001 aaagtagaaa ggactgacgg cggtatccat ataagtacgg atttgactga acgtccccag
   117061 gcggctgtcg gcaataataa tgccgagcgc caccagcacc gccagaataa ggcgaatctg
   117121 tagcgacggg ccacggctaa aaattggctt cataagttat gcgtattctc gtatcagacc
   117181 aggcagggta aacagacact tcccctgcct gcatccgatt actcttcgct gaacaggtcg
   117241 ccgccgtgca tgtcgatcat ttccagcgct ttgccgccac cgcgcgccac acaggtcagc
   117301 gggtcttcag caacaacgac tggaatgccg gtttcttcca ttaacaaacg gtcaaggtta
   117361 cgcagcagtg cgccaccacc ggtgagcacc atgccgcgct cggagatatc ggaagccagt
   117421 tccggcgggc actgttccag tgcaaccatt accgcgctca caataccggt cagcggttcc
   117481 tgcagtgctt cgaggatttc gttggagttc agggtaaaac cgcgtggaac accttctgca
   117541 aggttacggc cacgaacttc gatttcacgg acttcatcgc ccgggtaagc cgaaccgatt
   117601 tcgtgcttga tacgttctgc ggtggcttca ccgatcagag aaccgtaatt acgacgtaca
   117661 tagttgatga tagcttcgtc gaaacggtca ccaccaatgc gcacggaaga ggagtaaacc
   117721 acgccgttca aggagataac agcaacttca gtggtaccac caccgatatc aaccaccata
   117781 gaaccggtcg cttcagaaac cggcaggcca gcaccaattg cggcagccat cggttcttca
   117841 atcaggaaga cttcacgggc accagcgccc tgcgcggatt cacgaattgc gcggcgttca
   117901 acctgggtcg cgccaaccgg cacacaaacc agaacgcgcg ggcttggacg cataaagctg
   117961 ttgctgtgca cttgtttgat gaagtgctgg agcatttttt cagtcacgaa gaagtcggcg
   118021 ataacgccgt ctttcattgg gcgaatggca gcaatattgc ccggcgtacg gcccagcatc
   118081 tgcttcgcgt catgacctac tgcagctacg cttttcggtg aaccggcacg atcctgacga
   118141 atggccacca cggaaggctc attcaatacg atgccttgtc cttttacata aatgagggta
   118201 ttcgcagtac ccaggtcaat ggacaagtca ttggaaaaca tgccacgaaa ttttttcaac
   118261 atactaaggg ataatcctga aagctggggc ggaaaagaaa atccgcttac tttaccaacc
   118321 acacgcagca gcgacaaggc gcaaaaatca tctgctacgg tgaaaattag tgcagttcgt
   118381 ttcctttgtt acaaatctct gcctgagtcc agaaaggctt aatgcatcag cagcattcct
   118441 cgcctgtttg caaccgcgta aggtcattca tctgcatatg tgctgcaaca atctggcgag
   118501 cagacaagca cactcccatg agacgcagcg cgcgttattc tacgtgaaaa cggattaaac
   118561 ggcaggttaa accgagtatc tttgtgaata ttttttcacg ttagtatcaa gtggctgtga
   118621 ggacgcgaaa aaatcccctt gcccacctgt aacaccgcgc tgaatcaggg tctgccactc
   118681 gcttcgcgaa cgtacgccgg tggcgtaaac ctgggtgctg gtcccggagc aggcttccac
   118741 caggctttga accagcagct ggttctccgt tcgcttctca atgtttctga ccagccccgg
   118801 atggagcttg agtaactcaa cattaagttc tttgatccaa ctggtgctta ccagcgtcaa
   118861 accagcctgg ttgacggcta cccgtacccc taaagcattc actaaacgaa taacaggttg
   118921 taagcgactg atatgttgac caacatcggc ctctgcaagt tcaataatta tgcgtcttcg
   118981 ttgcgatttt tcacattgca ttaacgtatc gcgcagccaa cgctgaaaac gcgggcgaat
   119041 cagcgactca acggtaacct gaatcgccag attttcctct ggccagtaac gcaatagtgg
   119101 aataagacgg ctgatttgca ggcggtcata ctcttccgat aaaccaaact gcaagaccat
   119161 cggcatatac tctgccgagc taacctcttc attaccatcg aagatgcggc acatgagttc
   119221 acgatgatga acccggcctt cgcgagtaac cgccggtttt tgataaaggc gcgggccgcc
   119281 gcgactgagc atttgctcga taagcgtacg ccagcgaaca ttaccgcgtc ctttttcagg
   119341 caacgagtca tcgtaaatag cccagctatt gccgccctgc aatcccgcat tacgcgtggc
   119401 agactctgca tgttccatta cctgctcggt atcctgacca ctacgccagg cacagatacc
   119461 aatgtggatc atatcgtcgc gatcgagcat tttattgttc ggcaaggtat cgacggcttt
   119521 gattaactga ctggcgatgc tctctgcctc ttttaacgtc cggtgcggta acagcgcagc
   119581 aaaatcactg cggtggtaac gcgccagcag tgcgccaggg tagcgcatca taaatgtcga
   119641 tagcagattc gtcagagaga agaactgttc ttcaacctgg ctgtgccccc aggtatcact
   119701 caacatattg aaatccggca gacgaatcat catcacgatc ccgtgggtac ctactttctc
   119761 ttgatcttcc agtaacgttg ctaactgatt atcgaaaaag agtcggttat tgaggccggt
   119821 tttcatgtcc tgggcggcat aagagcggat cagcgtatca agacggctgt gttgttcgcg
   119881 tgcgttctga atttcacaaa gcagcgtatc cagcgcactg ctggttctgg gcggccattc
   119941 atagatggtt cccaacacat tagagccacg ctcaccgttt aagatacgag tagcccgggt
   120001 ttccagcaat tcttgcccgg caagttgccg ttgtaaccag cgtaccgcca ggaagagcat
   120061 aagaatgata aagccaatcg cccccgtgag cggcgcggta gtcattaaag aatggaaata
   120121 gttgcccatc ggatcctgat aaaccagacg cagcgacatc cccggatgct ttatcaacgg
   120181 aacgctcagt tcacgaaaca gatcgttggt gccaaccgga cgataactac catttctggc
   120241 cagggtataa acctgcttat caccatggag caggtctaca cgaacgatat ctgccgacat
   120301 cattaattcg gtaatttgtg gccttaatgt gctgaagtca ttcgacacaa ggtaggtatc
   120361 aatcgccgtc gccaccgcct gaacacgatg actaaactta tactgaatgg cgttgtagaa
   120421 acttagcgaa cagcccagca aagtcacaaa aattgttaac ccggtgagca gcgtgacaaa
   120481 ggccgaaaat ttcgtcgtta atctcatcct tgtgttaact ccgatagtga ggaagcgggc
   120541 atactagcaa atcagattta tctcgcaatt tattgcgctt catcggcttt gcttttccat
   120601 tagcgagtat agtcttcaga aattattttc caatccatca tgcacatgag gaccacttat
   120661 gcaggcgtta cttttagaac agcaggacgg caaaactctc gcatcagtac agactctgga
   120721 cgaaagtcgc ctgccggaag gcgatgtcac ggttgatgtt cactggtcga gcctgaacta
   120781 caaagatgcg ctggcgatta ccggtaaggg aaaaatcatc cgtaattttc cgatgattcc
   120841 tgggatcgat tttgccggaa ctgtacgcac cagcgaagat ccgcgttttc atgccggtca
   120901 ggaggtgtta ctcactggct ggggcgttgg tgaaaaccac tggggtgggc tggcggaaca
   120961 ggcgcgagtg aaaggtgact ggctggttgc catgccgcaa gggctggacg cgcgtaaagc
   121021 aatgattatc ggtactgccg gttttaccgc catgctgtgt gtgatggcgc tggaagatgc
   121081 cggtgttcgc ccgcaggatg gggagattgt cgtgacgggt gccagtggtg gcgtcggcag
   121141 taccgccgtg gcgctgctgc ataagttggg ttatcaggtc gttgctgttt ccggtcgcga
   121201 aagtacccat gaatatctga aaagtttagg tgccagccgt attcttcctc gcgatgagtt
   121261 tgctgaatcc cgtcctctgg aaaaacaagt ctgggctggg gcaattgaca ccgttggcga
   121321 caaagtgctg gcaaaagtgc tggcgcaaat gaattacggc ggctgcgtgg cggcctgtgg
   121381 tctggcgggt ggttttactc tgccaaccac ggtcatgcca tttattctgc gtaatgtccg
   121441 tttgcaaggg gtggattcag taatgacgcc accagcacgc cgcgcacaag cctggcagcg
   121501 actggtcgcc gatttaccgg aatcattcta tacccaggcg gcaaaagaga tatctctggc
   121561 agaggcaccg aagtttgccg aggccatcat taataaccag atccagggtc gcacgctggt
   121621 gaaggttaac taaccattta gcagggaata ataagagagg gaactcattt tgaaatcatt
   121681 attcaatcgg ttaacgggaa aagcggttag ccggacagct ttcgtcgaac atcttggtca
   121741 tgaagttgta caacatcatc caaactggaa agtcatgatt tcgactgacc ataaattgat
   121801 gcgcattgat gctccaccaa acagccatta ttgatacgcc tccgtcgcct gttaggttta
   121861 tggtgttttg ccttggcgac gctacgctta atctgttact gatttctggt accatggggg
   121921 gaataatctg attttatttg attgcaaatt aaccacttaa acctattgaa tgcggaacat
   121981 tgtcagtcgg ttaattttgc gtgctttagc attcacatct ttccggacga tgcagtgaaa
   122041 attgggcaat ccccagcaac cgctgcgtaa tgtcgtctat cttgtcgcga tcctggcatc
   122101 cctacattat ttgtggtctg tgaagattat ctcattgcag ccccccatct tcgcagggct
   122161 ggctttacag cttttcacct tacgttataa gaagttccgt cgatgatggc gctaatttcg
   122221 tgaattgtgc ggcttgttgc aaattacacg gtgttgaagg ttatttacat gttagctgtt
   122281 gattatcttc cctgataaga ccagtattta gctgccaatt gctacgaaat cgttataatg
   122341 tgcgacctcg tcctccctga cgcagttttt gcgctgcgga aaaggtgaca ttggcgcaac
   122401 gaaggtatat tttgtttttt gccggaggat agcagcagat cgctgcacaa tgtccgtcaa
   122461 gtctaacatt gacactctgg ggcaaaatag accggcgtcc cggcctgctg gaatttatcg
   122521 ctatgcatac agctgtcggg gcatacgctt tacagacggc ggtgaaacgc ctgtcacaat
   122581 cacactaaac aaagagtacg gaacccactc atggatattc gtaagattaa aaaactgatc
   122641 gagctggttg aagaatcagg catctccgaa ctggaaattt ctgaaggcga agagtcagta
   122701 cgcattagcc gtgcagctcc tgccgcaagt ttccctgtga tgcaacaagc ttacgctgca
   122761 cctatgatgc agcaaccagc tcaatctaac gcagccgctc cggcgaccgt tccttccatg
   122821 gaagcgccag cagcagcgga aatcagtggt cacatcgtac gttccccgat ggttggtact
   122881 ttctaccgca ccccaagccc ggacgcaaaa gcgttcatcg aagtgggtca gaaagtcaac
   122941 gtgggcgata ccctgtgcat cgttgaagcc atgaaaatga tgaaccagat cgaagcggac
   123001 aaatccggta ccgtgaaagc aattctggtc gaaagtggac aaccggtaga atttgacgag
   123061 ccgctggtcg tcatcgagta acgaggcgaa catgctggat aaaattgtta ttgccaaccg
   123121 cggcgagatt gcattgcgta ttcttcgtgc ctgtaaagaa ctgggcatca agactgtcgc
   123181 tgtgcactcc agtgcggatc gcgatctaaa acacgtatta ctggcagatg aaacggtctg
   123241 tatcggccct gctccgtcag taaaaagtta tctgaacatc ccggcaatca tcagcgccgc
   123301 tgaaatcacc ggcgcagtag caatccatcc gggttacggc ttcctctccg agaacgccaa
   123361 ttttgccgag caggttgaac gctccggctt tatcttcatc ggcccgaaag cagaaaccat
   123421 tcgcctgatg ggcgacaaag tatccgcaat cgcggcgatg aaaaaagcag gcgttccttg
   123481 cgtaccgggt tctgacggcc cgctgggcga cgatatggat aaaaaccgcg ccattgctaa
   123541 acgcattggt tatccggtaa ttatcaaagc ctccggcggc ggcggcggtc gcggtatgcg
   123601 cgtagtgcgc ggcgacgctg aactggcaca atccatctcc atgacccgtg cggaagcgaa
   123661 agctgctttc agcaacgata tggtttacat ggagaaatac ctggaaaatc ctcgccacgt
   123721 cgagattcag gtactggctg acggtcaggg caacgctatc tatctggcgg aacgtgactg
   123781 ctccatgcag cgccgccacc agaaagtggt cgaagaagca ccggctccgg gcattacccc
   123841 ggaactgcgt cgctacatcg gcgaacgttg cgctaaagcg tgtgttgata tcggctatcg
   123901 cggtgcaggt actttcgagt tcctgttcga aaacggcgag ttctatttca tcgaaatgaa
   123961 cacccgtatt caggtagaac acccggttac tgaaatgatc accggcgttg acctgatcaa
   124021 agaacagctg cgtatcgctg ccggtcaacc gctgtcgatc aagcaagaag aagttcacgt
   124081 tcgcggccat gcggtggaat gccgtatcaa cgccgaagat ccgaacacct tcctgccaag
   124141 tccgggcaaa atcacccgtt tccacgcacc tggtggtttt ggcgtacgtt gggagtctca
   124201 tatctacgcg ggctacaccg taccgccgta ctatgactca atgatcggta agctgatttg
   124261 ctacggtgaa aaccgtgacg tggcgattgc tcgcatgaag aatgcgctgc aggagctgat
   124321 catcgacggt atcaaaacca acgttgatct gcagatccgc atcatgaatg acgagaactt
   124381 ccagcatggt ggcactaaca tccactatct ggagaaaaaa ctcggtcttc aggaaaaata
   124441 atttgtgacg cgcggtgttt tcatcgcgct tagcaaagaa cgtaatcaac gccgggcctg
   124501 ttaatgctac ccggcgtttt tattgccgtt aactaacggt agcctgcaat ttccgctttc
   124561 aacgcgctaa tttcagcatc gccatcaaca attcccagct caaagcagta tttctttgtt
   124621 tcccctggct caataaaggg cagacgcccc tgcgctcgtt ctttatcacg accgtcagga
   124681 aaactattac tgacctccag tcccataaca tattcacccg cgcctaaatt tttccactgg
   124741 ataaagttgg gtagctgcat ggtgtcgaat ttctcgtaaa ttccccaccc cagttcggcg
   124801 ttcaccagcg caactttact catcccctga ctgtcgctat tcaacgtgag atagtaaacc
   124861 tgctcgtcaa aaccgggctg tggggtacaa atctccagat gactggttaa cccttccgcc
   124921 gcatgcagag ttcgtggcgt tatgctttta gccgacagca agatttctgc ctgtggcgat
   124981 aataaaggcc agccgtaatt aaagtgataa agaatttgca gcggctggcg ggtgaaacca
   125041 tgattagtca ccttgtcttc aatgcgcatt acattttcgc cataacggca gcgaatggtc
   125101 ctttccagcg tcagattttc accgaacaat cgtgtttccc gtactttgcc attgatgaca
   125161 aattcaatgt tgtcgtcggt ccgttctgtg cgatacccca cctcttctgc tggagtcgca
   125221 gccaaacgcc cgtgtaaccc cagcgtttct ccttcatcct cacagggcgt gccaatgtag
   125281 ctaagtccgc aggtagtcag taaccccgcg taaaagctac gtaaaaaccc gttgccctga
   125341 gactcaaaaa aagcgggggc gacaacaccg ttgggggtga taaaagagag cgcagtgtcc
   125401 ttgtagctgg cccatgcaat atccagcgcg cgatctttta ccaccgtaaa tgccagtccc
   125461 gcaccagtac ggacatcaaa cgcttctgta ccctttgctc tgccactacg taactcatag
   125521 tgtttaattc cggcaacctg cgagagatcg ccaatccacg accaaatttc gtttttcatt
   125581 gtgctgcttc ccgaatggac atgtcatggc gctggatgaa cgtttcgatt tccgcaaggc
   125641 gagacgttcc ctccattggg ccacgtcggg ttaccgccag tgcaccgcag gcgttggcat
   125701 attgtaatgc ccgatgggcg tcaaacccta gctggcggca ggcaatccat gcgccaccaa
   125761 aacagtctcc cgcaccggtc gggtcaactt cttccaccgg atagctctca acatgaaact
   125821 gctcattcgc ggagtagtaa ctggctcctt gattgccgcg cttcacaatc acctctttaa
   125881 caccttcttc aagaaaacca gcgattgcac gctccggcgt cgagtgtggc gacaagagca
   125941 acacttctcc ctcactgggc atatagatat ccgttaactc aagaacaaag tggagggcgt
   126001 cacgcatttc tggaatatcg agcatctctt tccgaatatt agggtcaaaa gaaataacgc
   126061 caccattctc ttttacaatg gttaccgctt tctttactgc gtctaccata tggaatgaaa
   126121 acagggatga acccataata tgaaaatggg tacagtcctt cagaatattt tcatcaacat
   126181 gctgcgccga taatttaccg caagcggcat tcttgatatt aaaaataaaa tcacgatcgc
   126241 cagagttatg ataagtaaca aaggcactcc cggtagcttc cagaggtaag actgaaatcc
   126301 ccctgatatc cacaccgtca gcagccagac gatgtatatt aatatcacca aagccatcat
   126361 tccccacaca actaatgatg ccgcacggaa cgccaagacg tgtcacctgg tcgataaaaa
   126421 tagcgggcgc accactggga taagggccgt tccagatacc tggttgatca aatggctggc
   126481 cgattttact cgccataatt tcaaccagaa tttctccaat tgtgaagacc ttactcatat
   126541 gattaattcc ttgaaatgat tagctgcgtt ttcttttatt tcttacgtca aaataaacgg
   126601 ctacaagaat gactaatcct ttaaccacca tctggtaaaa ataaggaaca ttcattaagt
   126661 tcatgccatt attaataacg ccgatggtta atgcgccgat caccgtcccc accacggttc
   126721 catagccgcc gttcaggctg gtgcctccca gtacagctgc ggcaatagca tccagttcgt
   126781 agctcaacgc ggaattcggt tgcccggaat agaggcgtga ggcaaggatc atcccgctga
   126841 tacctgccag cacggctgta atcataaaga ctttaatttt caagcgggtg acatttatcc
   126901 ctgaataaat tgctgcttct ttattaccac cagtgatata aatttttcgt ccaaaggttg
   126961 ttttagtaag aatgaagtgg tttatcagca ataagatgat taatatccat ataggaatag
   127021 gtatatagag aaattctcca ttccccagcg ccagaaaagc atcatcttta atcatgatgg
   127081 gcataccatc ggtcactata taagcgatac cacggaagat ccccatagta gcaacggtga
   127141 caataaacga agggatcccc gctatagccg tcaagccacc atttatcatc cccagagcca
   127201 gtgctgcaat aatggataat gggaccaata aaacaaatgg aattccccat gaagccccaa
   127261 gcgcagcaaa tgtgccaatc aatgcaatgg ttgaaccgac cgataaatca atatcgccaa
   127321 gcaaaagcac ataagtcatt ccataggcgg taattgcaat tatcgaaacc tgaagaataa
   127381 tattagtaat gttattggcg gtaaaaaaac tgtcattaat aaaactgaac agaatgatta
   127441 acaccaccat tccactgagg ataccaccat attgcctgaa gactttttta aaattatttg
   127501 caatgggaat ttccgaaggt ttcggactgg caagagtttt ctctgtcatt taacgtactc
   127561 ctgttgcagc ggtcattacc tgtgtctgcg ttaaattttc acatgaataa atatcggcaa
   127621 tcgttttatt acgcattacc accagacgat cgctaagtgc catgacttct ggcagttcag
   127681 aggacaccag aataattgct gtccctgccg cagccagttg acgtatcacg ttatagattt
   127741 caaattttgc gccgacatcc actccgcgcg ttggctcatc gagcaataaa atacgcggag
   127801 ttttctccag ccacttcgat aacacgattt tctgttgatt accgccgctc agtgctccca
   127861 ctgcctgatg cacagaactc actttagtgt tcattctggc aattgattgc agcgtcttcg
   127921 cctcttcttg tcggtgattg atgagtccca tccgcgaagc gttatattga aacgccacat
   127981 ggtgcgtgtt catattgacg tcgtgcatca gcactaaccc ttcttccttg cggttttcgg
   128041 tcacaaaagc aatgccatga tcaatagcct gtttgggatt agcgatcctg acttccttac
   128101 catcaatcaa cacagtcccg tgatacgaaa cgaggccaaa tagcgccttc atcacatcgg
   128161 aacgcccggc ccccaccagg ccaaaaaatc cgaggacttc ccccggacgc acagcgaaac
   128221 tgacctcttt aaaaagcgaa ggatgggaaa gattttttac ttccagtttt gcaggaacat
   128281 tttcatccgt tttttcccca agacgtgctg gccaaatatt tttcatttcc cgaccaacca
   128341 tttgggcaat caagtgctgg atatcactgt ttttcttttc atgcgtagcg atatactcac
   128401 cgtcgcggat caccgaaata tcatctgaaa tccgcataat ttcatccatg cgatgagaga
   128461 tataaataac cgcacagcct cgctgcttta atttttcaat aatgttaaac agaatctccg
   128521 cttcattatc acttagcgag gatgtcggtt catccataat cacgacatcc gctgcatagc
   128581 ttaaggcttt ggcaatttct accagttgcc tatgtgccat cgacatattg ccgaccattt
   128641 ccagagggtc tagtgggatg cccagttccg cgaggaggtt ctggcaatct gcgagcattt
   128701 ttttatcgtt aatgatgcca aaacgtcgcg gttcattatt ggcataaata ttttccgcca
   128761 cggtcatatt attactcagg ctcagttcct gaaaaataat cgcaatccct aactggcgag
   128821 aatgctgcgg attattaatg gtgatggttt caccacgtag tgttatcgtt ccctgggttg
   128881 gcggataaac accggccagt attttcatta atgtcgattt accggcacca ttctcgccta
   128941 ataaagtatg cacccgccct ttacgtaaag agagtgtaat atcttttaat gcctgaacat
   129001 gaccaaatgt cttcgcaata tgcgaaacat caagaataac atccgtcata atgacctcgg
   129061 tgtcacaaca aaacgccaat aacgttttgt tgtgaggtga agatatttac tatttacctg
   129121 tataaacgcc gggttgtacc gggatcattt tttctaccgg tttaccattg aggtgatcaa
   129181 cagcggcatc aatggcttta gcctccattt gatcgggata ttgccgaatc acggccgcaa
   129241 acgttttttc actgtcaacg gcatcacgcg cttctttcat gccgtcaaaa ccaatgattt
   129301 tgatgttgtc gttatgcgca gattttgctg cgataaccgc tgccagcgcc gcatcatcac
   129361 caaagccaaa cagaccattc aggttggcgt ttgcctgcat aatgttttgc gcagtcgtta
   129421 acgcttcagc ccgcgtaata ccaggctgtt cagcaacaat tttaatatcc ggtgtatcgc
   129481 ttaacccttt cttaaaacca tcaacgcgcg caaccaccga ttgtagtgcc ggataagaaa
   129541 taatggctac agtaccttta ccatgcaaaa gtttacccat taacttgcct gcttcaacgc
   129601 ctcccgcata gttatcggtc gcaacatgcg atacgaccgg aacgccttcc gcagcgacgt
   129661 caacggtgat aacgggaata ccggccttat cggctttaat tatcgctgcc tggacgcctt
   129721 tcgaatcaac cggagagata ataatggcat ccactttttt agtaataaaa tcctcaacat
   129781 cagaaagttg tttgtttaaa tcctgattgg caatagagac acttaaattg acattgtctt
   129841 tttttgcctc ttctttcatg gcattcgcaa gatcaatata gaaaggatgt tgttgtgtta
   129901 ataatgacgc accaatttta tactcttttg ccaacgaact ttgcgagaac a
//
LOCUS       DAZFRE010000002        99167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFRE010000002 DAZFRE010000000
VERSION     DAZFRE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 99167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.002"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            113..322
                     /gene="fumD"
                     /locus_tag="ACHKUI_000133"
     CDS             113..322
                     /gene="fumD"
                     /locus_tag="ACHKUI_000133"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6596417.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            778..1404
                     /gene="ydhY"
                     /locus_tag="ACHKUI_000134"
     CDS             778..1404
                     /gene="ydhY"
                     /locus_tag="ACHKUI_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6596418.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKNITV"
     gene            1425..3527
                     /gene="ydhV"
                     /locus_tag="ACHKUI_000135"
     CDS             1425..3527
                     /gene="ydhV"
                     /locus_tag="ACHKUI_000135"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6596419.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYTIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLVAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQNPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3540..4178
                     /gene="ydhW"
                     /locus_tag="ACHKUI_000136"
     CDS             3540..4178
                     /gene="ydhW"
                     /locus_tag="ACHKUI_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6596420.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4242..4910
                     /gene="ydhX"
                     /locus_tag="ACHKUI_000137"
     CDS             4242..4910
                     /gene="ydhX"
                     /locus_tag="ACHKUI_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6596421.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTAASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4907..5692
                     /gene="phsC"
                     /locus_tag="ACHKUI_000138"
     CDS             4907..5692
                     /gene="phsC"
                     /locus_tag="ACHKUI_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6596422.1"
                     /translation="MNLSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5696..6508
                     /gene="ydhT"
                     /locus_tag="ACHKUI_000139"
     CDS             5696..6508
                     /gene="ydhT"
                     /locus_tag="ACHKUI_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6596423.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(6520..8124)
                     /gene="ydhS"
                     /locus_tag="ACHKUI_000140"
     CDS             complement(6520..8124)
                     /gene="ydhS"
                     /locus_tag="ACHKUI_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6596424.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKRENHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(8250..8555)
                     /gene="ydhR"
                     /locus_tag="ACHKUI_000141"
     CDS             complement(8250..8555)
                     /gene="ydhR"
                     /locus_tag="ACHKUI_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6596425.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(8664..8740)
                     /locus_tag="ACHKUI_000142"
     tRNA            complement(8664..8740)
                     /locus_tag="ACHKUI_000142"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8704..8706),aa:Val,seq:gac)
     gene            complement(8745..8821)
                     /locus_tag="ACHKUI_000143"
     tRNA            complement(8745..8821)
                     /locus_tag="ACHKUI_000143"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8785..8787),aa:Val,seq:gac)
     gene            9130..10386
                     /gene="ydhQ"
                     /locus_tag="ACHKUI_000144"
     CDS             9130..10386
                     /gene="ydhQ"
                     /locus_tag="ACHKUI_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6596426.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10427..11800)
                     /gene="mdtK"
                     /locus_tag="ACHKUI_000145"
     CDS             complement(10427..11800)
                     /gene="mdtK"
                     /locus_tag="ACHKUI_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6596427.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIGSMQNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPVVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            12015..12656
                     /gene="ribE"
                     /locus_tag="ACHKUI_000146"
     CDS             12015..12656
                     /gene="ribE"
                     /locus_tag="ACHKUI_000146"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6596428.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTQA"
     gene            complement(12696..13844)
                     /gene="cfa"
                     /locus_tag="ACHKUI_000147"
     CDS             complement(12696..13844)
                     /gene="cfa"
                     /locus_tag="ACHKUI_000147"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6596429.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14135..15346)
                     /gene="punC"
                     /locus_tag="ACHKUI_000148"
     CDS             complement(14135..15346)
                     /gene="punC"
                     /locus_tag="ACHKUI_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6596430.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            15459..16391
                     /gene="punR"
                     /locus_tag="ACHKUI_000149"
     CDS             15459..16391
                     /gene="punR"
                     /locus_tag="ACHKUI_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6596431.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYSF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16388..17413)
                     /gene="purR"
                     /locus_tag="ACHKUI_000150"
     CDS             complement(16388..17413)
                     /gene="purR"
                     /locus_tag="ACHKUI_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6596432.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17712..17801
                     /gene="cydH"
                     /locus_tag="ACHKUI_000151"
                     /gene_synonym="ynhF"
     CDS             17712..17801
                     /gene="cydH"
                     /locus_tag="ACHKUI_000151"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6596433.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17967..19136
                     /gene="ydhP"
                     /locus_tag="ACHKUI_000152"
     CDS             17967..19136
                     /gene="ydhP"
                     /locus_tag="ACHKUI_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6596434.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLVIPFLARNEFGAAMSMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSVRKQPETVCVANS"
     gene            complement(19282..19863)
                     /gene="sodB"
                     /locus_tag="ACHKUI_000153"
     CDS             complement(19282..19863)
                     /gene="sodB"
                     /locus_tag="ACHKUI_000153"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6596435.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19991..20806)
                     /gene="mepH"
                     /locus_tag="ACHKUI_000154"
     CDS             complement(19991..20806)
                     /gene="mepH"
                     /locus_tag="ACHKUI_000154"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6596436.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     NFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21141..21488
                     /gene="grxD"
                     /locus_tag="ACHKUI_000155"
     CDS             21141..21488
                     /gene="grxD"
                     /locus_tag="ACHKUI_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6596437.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21657..21767)
                     /locus_tag="ACHKUI_000156"
                     /pseudo
     CDS             complement(21657..21767)
                     /locus_tag="ACHKUI_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(21860..22507)
                     /gene="rnt"
                     /locus_tag="ACHKUI_000157"
     CDS             complement(21860..22507)
                     /gene="rnt"
                     /locus_tag="ACHKUI_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6596438.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(22610..23017)
                     /gene="gloA"
                     /locus_tag="ACHKUI_000158"
     CDS             complement(22610..23017)
                     /gene="gloA"
                     /locus_tag="ACHKUI_000158"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6596439.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(23098..24195)
                     /gene="nemA"
                     /locus_tag="ACHKUI_000159"
     CDS             complement(23098..24195)
                     /gene="nemA"
                     /locus_tag="ACHKUI_000159"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6596440.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(24232..24831)
                     /gene="nemR"
                     /locus_tag="ACHKUI_000160"
     CDS             complement(24232..24831)
                     /gene="nemR"
                     /locus_tag="ACHKUI_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6596441.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24934..25173
                     /gene="ydhL"
                     /locus_tag="ACHKUI_000161"
     CDS             24934..25173
                     /gene="ydhL"
                     /locus_tag="ACHKUI_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6596442.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKSAPSDEPEQPSLF"
     gene            25222..26118
                     /gene="ydhF"
                     /locus_tag="ACHKUI_000162"
     CDS             25222..26118
                     /gene="ydhF"
                     /locus_tag="ACHKUI_000162"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO6596443.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            26199..26720
                     /gene="sodC"
                     /locus_tag="ACHKUI_000163"
     CDS             26199..26720
                     /gene="sodC"
                     /locus_tag="ACHKUI_000163"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6596444.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26729..28741)
                     /gene="ydhK"
                     /locus_tag="ACHKUI_000164"
     CDS             complement(26729..28741)
                     /gene="ydhK"
                     /locus_tag="ACHKUI_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6596445.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMSKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28741..29598)
                     /gene="ydhJ"
                     /locus_tag="ACHKUI_000165"
     CDS             complement(28741..29598)
                     /gene="ydhJ"
                     /locus_tag="ACHKUI_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6596446.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNFYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSGLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVHFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(29601..29837)
                     /gene="ydhI"
                     /locus_tag="ACHKUI_000166"
     CDS             complement(29601..29837)
                     /gene="ydhI"
                     /locus_tag="ACHKUI_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6596447.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            30038..30472
                     /gene="slyA"
                     /locus_tag="ACHKUI_000167"
     CDS             30038..30472
                     /gene="slyA"
                     /locus_tag="ACHKUI_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6596448.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(30519..30986)
                     /gene="slyB"
                     /locus_tag="ACHKUI_000168"
     CDS             complement(30519..30986)
                     /gene="slyB"
                     /locus_tag="ACHKUI_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6596449.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            31260..32369
                     /gene="anmK"
                     /locus_tag="ACHKUI_000169"
     CDS             31260..32369
                     /gene="anmK"
                     /locus_tag="ACHKUI_000169"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6596450.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLSGNLPSVTGASQETVLGAIFPANP"
     gene            32473..32796
                     /gene="mliC"
                     /locus_tag="ACHKUI_000170"
     CDS             32473..32796
                     /gene="mliC"
                     /locus_tag="ACHKUI_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6596451.1"
                     /translation="MKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTVKL
                     NNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNCQL
                     QNPQR"
     gene            32855..33511
                     /gene="pdxH"
                     /locus_tag="ACHKUI_000171"
     CDS             32855..33511
                     /gene="pdxH"
                     /locus_tag="ACHKUI_000171"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6596452.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33640..34914
                     /gene="tyrS"
                     /locus_tag="ACHKUI_000172"
     CDS             33640..34914
                     /gene="tyrS"
                     /locus_tag="ACHKUI_000172"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6596453.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPCAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34976..35836
                     /gene="pdxY"
                     /locus_tag="ACHKUI_000173"
     CDS             34976..35836
                     /gene="pdxY"
                     /locus_tag="ACHKUI_000173"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO6596454.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35880..36485)
                     /gene="gstA"
                     /locus_tag="ACHKUI_000174"
     CDS             complement(35880..36485)
                     /gene="gstA"
                     /locus_tag="ACHKUI_000174"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6596455.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(36591..38093)
                     /gene="dtpA"
                     /locus_tag="ACHKUI_000175"
     CDS             complement(36591..38093)
                     /gene="dtpA"
                     /locus_tag="ACHKUI_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6596456.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(38704..39339)
                     /gene="nth"
                     /locus_tag="ACHKUI_000176"
     CDS             complement(38704..39339)
                     /gene="nth"
                     /locus_tag="ACHKUI_000176"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6596457.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(39339..40034)
                     /gene="rsxE"
                     /locus_tag="ACHKUI_000177"
     CDS             complement(39339..40034)
                     /gene="rsxE"
                     /locus_tag="ACHKUI_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6596458.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(40038..40658)
                     /gene="rsxG"
                     /locus_tag="ACHKUI_000178"
     CDS             complement(40038..40658)
                     /gene="rsxG"
                     /locus_tag="ACHKUI_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6596459.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(40662..41720)
                     /gene="rsxD"
                     /locus_tag="ACHKUI_000179"
     CDS             complement(40662..41720)
                     /gene="rsxD"
                     /locus_tag="ACHKUI_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6596460.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDTIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLATLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGMAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(41721..43850)
                     /gene="rsxC"
                     /locus_tag="ACHKUI_000180"
     CDS             complement(41721..43850)
                     /gene="rsxC"
                     /locus_tag="ACHKUI_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6596461.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDNVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVE
                     AAIARAKARKLEQQQQANAEPEEQVDPRKAAIEAAIARAKARKLEQQQANAEPEEQID
                     PRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(43843..44421)
                     /gene="rsxB"
                     /locus_tag="ACHKUI_000181"
     CDS             complement(43843..44421)
                     /gene="rsxB"
                     /locus_tag="ACHKUI_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6596462.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(44421..45002)
                     /gene="rsxA"
                     /locus_tag="ACHKUI_000182"
     CDS             complement(44421..45002)
                     /gene="rsxA"
                     /locus_tag="ACHKUI_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6596463.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(45079..45519)
                     /gene="ydgK"
                     /locus_tag="ACHKUI_000183"
     CDS             complement(45079..45519)
                     /gene="ydgK"
                     /locus_tag="ACHKUI_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6596464.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(45605..45820)
                     /gene="ydgT"
                     /locus_tag="ACHKUI_000184"
     CDS             complement(45605..45820)
                     /gene="ydgT"
                     /locus_tag="ACHKUI_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6596465.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWRYVQ"
     gene            complement(46093..46218)
                     /gene="blr"
                     /locus_tag="ACHKUI_000185"
     CDS             complement(46093..46218)
                     /gene="blr"
                     /locus_tag="ACHKUI_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6596466.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            46461..47501
                     /gene="ydgJ"
                     /locus_tag="ACHKUI_000186"
     CDS             46461..47501
                     /gene="ydgJ"
                     /locus_tag="ACHKUI_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6596467.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(47537..48538)
                     /gene="add"
                     /locus_tag="ACHKUI_000187"
     CDS             complement(47537..48538)
                     /gene="add"
                     /locus_tag="ACHKUI_000187"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6596468.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(48642..49814)
                     /gene="malY"
                     /locus_tag="ACHKUI_000188"
     CDS             complement(48642..49814)
                     /gene="malY"
                     /locus_tag="ACHKUI_000188"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6596469.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQPDGWFCDMGKLEAVLAK
                     PECKILLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFIRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(49824..51416)
                     /gene="malX"
                     /locus_tag="ACHKUI_000189"
     CDS             complement(49824..51416)
                     /gene="malX"
                     /locus_tag="ACHKUI_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6596470.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            51591..52619
                     /gene="malI"
                     /locus_tag="ACHKUI_000190"
     CDS             51591..52619
                     /gene="malI"
                     /locus_tag="ACHKUI_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6596471.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            52731..53498
                     /gene="hdhA"
                     /locus_tag="ACHKUI_000191"
     CDS             52731..53498
                     /gene="hdhA"
                     /locus_tag="ACHKUI_000191"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6596472.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            53727..54317
                     /gene="uidR"
                     /locus_tag="ACHKUI_000192"
     CDS             53727..54317
                     /gene="uidR"
                     /locus_tag="ACHKUI_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6596473.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            54707..56518
                     /gene="uidA"
                     /locus_tag="ACHKUI_000193"
     CDS             54707..56518
                     /gene="uidA"
                     /locus_tag="ACHKUI_000193"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO6596474.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNHVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            56515..57885
                     /gene="uidB"
                     /locus_tag="ACHKUI_000194"
     CDS             56515..57885
                     /gene="uidB"
                     /locus_tag="ACHKUI_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6596475.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISIFAVSASSLFYVRYVLNTGLFTVLVLVQNLVGT
                     VASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQGV
                     TMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYIA
                     NQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQL
                     INDITS"
     gene            57924..59189
                     /gene="uidC"
                     /locus_tag="ACHKUI_000195"
     CDS             57924..59189
                     /gene="uidC"
                     /locus_tag="ACHKUI_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO6596476.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLRTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(59234..60742)
                     /gene="ydgA"
                     /locus_tag="ACHKUI_000196"
     CDS             complement(59234..60742)
                     /gene="ydgA"
                     /locus_tag="ACHKUI_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6596477.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(60843..62018)
                     /gene="manA"
                     /locus_tag="ACHKUI_000197"
     CDS             complement(60843..62018)
                     /gene="manA"
                     /locus_tag="ACHKUI_000197"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6596478.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            62217..63863
                     /gene="fumB"
                     /locus_tag="ACHKUI_000198"
     CDS             62217..63863
                     /gene="fumB"
                     /locus_tag="ACHKUI_000198"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6596479.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            64006..65409
                     /gene="fumC"
                     /locus_tag="ACHKUI_000199"
     CDS             64006..65409
                     /gene="fumC"
                     /locus_tag="ACHKUI_000199"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6596480.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAASKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(65406..66335)
                     /gene="tus"
                     /locus_tag="ACHKUI_000200"
     CDS             complement(65406..66335)
                     /gene="tus"
                     /locus_tag="ACHKUI_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6596481.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEIKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(66411..67712)
                     /gene="rstB"
                     /locus_tag="ACHKUI_000201"
     CDS             complement(66411..67712)
                     /gene="rstB"
                     /locus_tag="ACHKUI_000201"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6596482.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVEISLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(67716..68435)
                     /gene="rstA"
                     /locus_tag="ACHKUI_000202"
     CDS             complement(67716..68435)
                     /gene="rstA"
                     /locus_tag="ACHKUI_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6596483.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            68564..68899
                     /gene="ydgC"
                     /locus_tag="ACHKUI_000203"
     CDS             68564..68899
                     /gene="ydgC"
                     /locus_tag="ACHKUI_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6596484.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(68896..69618)
                     /gene="folM"
                     /locus_tag="ACHKUI_000204"
     CDS             complement(68896..69618)
                     /gene="folM"
                     /locus_tag="ACHKUI_000204"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6596485.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGSRHLR"
     gene            complement(69655..71037)
                     /gene="ydgI"
                     /locus_tag="ACHKUI_000205"
     CDS             complement(69655..71037)
                     /gene="ydgI"
                     /locus_tag="ACHKUI_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6596486.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(71223..72167)
                     /gene="ydgH"
                     /locus_tag="ACHKUI_000206"
     CDS             complement(71223..72167)
                     /gene="ydgH"
                     /locus_tag="ACHKUI_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6596487.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIIQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            72691..74223
                     /gene="pntA"
                     /locus_tag="ACHKUI_000207"
     CDS             72691..74223
                     /gene="pntA"
                     /locus_tag="ACHKUI_000207"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6596488.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            74234..75622
                     /gene="pntB"
                     /locus_tag="ACHKUI_000208"
     CDS             74234..75622
                     /gene="pntB"
                     /locus_tag="ACHKUI_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6596489.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(75647..76681)
                     /gene="tqsA"
                     /locus_tag="ACHKUI_000209"
     CDS             complement(75647..76681)
                     /gene="tqsA"
                     /locus_tag="ACHKUI_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO6596490.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            77093..77458
                     /gene="mdtJ"
                     /locus_tag="ACHKUI_000210"
     CDS             77093..77458
                     /gene="mdtJ"
                     /locus_tag="ACHKUI_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6596491.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLTKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            77445..77774
                     /gene="mdtI"
                     /locus_tag="ACHKUI_000211"
     CDS             77445..77774
                     /gene="mdtI"
                     /locus_tag="ACHKUI_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6596492.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(77813..78634)
                     /gene="ydgD"
                     /locus_tag="ACHKUI_000212"
     CDS             complement(77813..78634)
                     /gene="ydgD"
                     /locus_tag="ACHKUI_000212"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6596493.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVAGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(78734..78817)
                     /locus_tag="ACHKUI_000213"
     CDS             complement(78734..78817)
                     /locus_tag="ACHKUI_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596494.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(78910..79218)
                     /gene="asr"
                     /locus_tag="ACHKUI_000214"
     CDS             complement(78910..79218)
                     /gene="asr"
                     /locus_tag="ACHKUI_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6596495.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            79489..79593
                     /locus_tag="ACHKUI_000215"
     CDS             79489..79593
                     /locus_tag="ACHKUI_000215"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6596496.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(79643..80896)
                     /gene="ynfM"
                     /locus_tag="ACHKUI_000216"
     CDS             complement(79643..80896)
                     /gene="ynfM"
                     /locus_tag="ACHKUI_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6596497.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            81003..81896
                     /gene="ynfL"
                     /locus_tag="ACHKUI_000217"
     CDS             81003..81896
                     /gene="ynfL"
                     /locus_tag="ACHKUI_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6596498.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVTIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            82031..83251
                     /gene="mlc"
                     /locus_tag="ACHKUI_000218"
                     /gene_synonym="dgsA"
     CDS             82031..83251
                     /gene="mlc"
                     /locus_tag="ACHKUI_000218"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6596499.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            83376..84071
                     /gene="bioD"
                     /locus_tag="ACHKUI_000219"
     CDS             83376..84071
                     /gene="bioD"
                     /locus_tag="ACHKUI_000219"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6596500.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(84024..85316)
                     /gene="clcB"
                     /locus_tag="ACHKUI_000220"
     CDS             complement(84024..85316)
                     /gene="clcB"
                     /locus_tag="ACHKUI_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6596501.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNNSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(85475..86089)
                     /gene="dmsD"
                     /locus_tag="ACHKUI_000221"
     CDS             complement(85475..86089)
                     /gene="dmsD"
                     /locus_tag="ACHKUI_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6596502.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDSWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(86132..86986)
                     /gene="ynfH"
                     /locus_tag="ACHKUI_000222"
     CDS             complement(86132..86986)
                     /gene="ynfH"
                     /locus_tag="ACHKUI_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO6596503.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGDSGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(86988..87605)
                     /gene="ynfG"
                     /locus_tag="ACHKUI_000223"
     CDS             complement(86988..87605)
                     /gene="ynfG"
                     /locus_tag="ACHKUI_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO6596504.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNGNSRPTGDTTGYLANPEEV"
     gene            complement(87616..90039)
                     /gene="ynfF"
                     /locus_tag="ACHKUI_000224"
     CDS             complement(87616..90039)
                     /gene="ynfF"
                     /locus_tag="ACHKUI_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO6596505.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWILSEVAKSL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQTNPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNTNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(90100..92526)
                     /gene="ynfE"
                     /locus_tag="ACHKUI_000225"
     CDS             complement(90100..92526)
                     /gene="ynfE"
                     /locus_tag="ACHKUI_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO6596506.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAEAAV
                     QQANEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAHKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(92725..93030)
                     /gene="ynfD"
                     /locus_tag="ACHKUI_000226"
     CDS             complement(92725..93030)
                     /gene="ynfD"
                     /locus_tag="ACHKUI_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6596507.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            93138..93848
                     /gene="ynfC"
                     /locus_tag="ACHKUI_000227"
     CDS             93138..93848
                     /gene="ynfC"
                     /locus_tag="ACHKUI_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6596508.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(93851..94411)
                     /gene="speG"
                     /locus_tag="ACHKUI_000228"
     CDS             complement(93851..94411)
                     /gene="speG"
                     /locus_tag="ACHKUI_000228"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6596509.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINDQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(94446..94787)
                     /gene="ynfB"
                     /locus_tag="ACHKUI_000229"
     CDS             complement(94446..94787)
                     /gene="ynfB"
                     /locus_tag="ACHKUI_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6596510.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            94922..95248
                     /gene="ynfA"
                     /locus_tag="ACHKUI_000230"
     CDS             94922..95248
                     /gene="ynfA"
                     /locus_tag="ACHKUI_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6596511.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            95454..96668
                     /gene="rspA"
                     /locus_tag="ACHKUI_000231"
     CDS             95454..96668
                     /gene="rspA"
                     /locus_tag="ACHKUI_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO6596512.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            96680..97699
                     /gene="rspB"
                     /locus_tag="ACHKUI_000232"
     CDS             96680..97699
                     /gene="rspB"
                     /locus_tag="ACHKUI_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO6596513.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCRHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            97757..99124
                     /gene="ydfJ"
                     /locus_tag="ACHKUI_000233"
     CDS             97757..99124
                     /gene="ydfJ"
                     /locus_tag="ACHKUI_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO6596514.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQ
                     PSRSL"
BASE COUNT        25130 a        24010 c        25562 g        24465 t
ORIGIN      
        1 attgctacag ttaaaataat ttatcacgtt atttgtctga ttttgtttga tttttgttta
       61 attggttaga ttgatatatg ctgatagttg gcatgataaa taggtataaa gaatgggaaa
      121 ccggacaaaa gaggatgaac tgtaccgaga aatgtgcaga gttgtcggta aagttgtgct
      181 ggaaatgcgc gatctggggc aggaaccaaa gcatattgtt atcgcaggcg tattacgtac
      241 agcattagcc aataaacgca ttcagcgcag tgagctggaa aagcaggcga tggaaacagt
      301 cattaacgca ctggtaaaat gaccattcag gatgaggcgg taagcttcat cctgatattg
      361 ttagttcagt tgcggtccag ttaatcattc taaataacaa ctgctgaaat tatacgaatt
      421 tcggtgtata ttttaattta tagcgataaa gttattttta ttttctgcga tgaacttcac
      481 aaataattgt cggttaagtt aaatttattt caaatgataa taaatatcag ttcacttata
      541 atttcttgct ttgtgatttt aattgataac gatcaatgtt aagagcccag aataatcaca
      601 ttattttttc tcctagccat aaaggattag tttatcagcg ttaaatcttt tctcatacac
      661 tatcgggtta gggaaaatat ctggtgttga ggacagagtc agcagcgtat tagttgagtg
      721 cctaatatca ggctgctgaa ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg
      781 aacccggttg atcgtccact attaaatatt ggcttaacgc gattagaatt cttgcgtata
      841 tcaggaaaag gccttgctgg tttaacaatt gcccctgctt tactgtcact tttgggttgc
      901 aaacaggaag atattgatag cggcacagta gggttgataa atacacccaa aggggtgctg
      961 gtgacccaac gtgcgcgatg cacgggttgc caccgctgtg aaatctcttg taccaacttc
     1021 aacgatggct cagtagggac attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt
     1081 ggcgacaacg gggttggctc tggcggcggc ctgtatggcg atctcaacta taccgcggat
     1141 acctgccgtc aatgcaaaga gccgcaatgc atgaacgtct gcccgattgg tgcgattacc
     1201 tggcagcaga aagaaggctg tattaccgtc gatcataaac gttgtattgg ctgtagcgcc
     1261 tgtactacgg catgcccgtg gatgatggcc accgtaaata ctgaaagtaa aaaatcctcg
     1321 aaatgtgtgt tatgcggtga atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc
     1381 gagtggaaaa atattactgt gtgaatcttg caaaggaaaa aatcatggct aacggttgga
     1441 caggtaatat attaagagtc aatctcacga caggaaatat taccctcgaa gattccagta
     1501 agtttaaaag ttttgtcggt ggcatgggct tcggctacaa aattatgtat gacgaagtac
     1561 cgccaggcac gaaacctttc gatgaagcga ataaattagt ttttgctacc ggcccattaa
     1621 ctggatctgg tgccccctgt agttctcgcg taaatatcac ctcactttct acttttacca
     1681 aaggaaattt agtcgtcgat gcccatatgg ggggcttttt tgcagcgcaa atgaaattcg
     1741 ctggatacga cgtcattatt atcgaaggga aagcgaaatc accggtatgg ctgaagatta
     1801 aagatgacaa agttagcctt gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa
     1861 cgacggaaga aatttgtcgc ttgaccagtc cggaaacctg tgtggcggct attggtcagg
     1921 ctggggaaaa ccttgttcct ctctctggca tgttgaatag ccgtaaccac agcggcggtg
     1981 cgggaactgg cgcaataatg ggttcgaaaa acctgaaagc gattgcggtt gaagggacga
     2041 aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct caacgattac atgatgactg
     2101 aacttatcgg tgcgaataac aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt
     2161 attcagatcc caagtcacgt tggacagcac gtaaagggct gttttggggc gcggctgaag
     2221 gtggtccgat tgaaacgggg gaaattccgc caggcaatca gaatacggtc ggctttcgta
     2281 cctataaatc cgtttttgac ttaggtccgg cggcagagaa atacacagta aaaatgagcg
     2341 gctgccactc ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt
     2401 ttggcgtgcc tggcaccggt ggtaacactt gtgtagcaaa ctttgtccat accactatct
     2461 tcccaaacgg gccgaaagat tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg
     2521 ttggtctgaa tctattcgat gactacggcc tatggtgtaa ctatgggcag ctgcatcgcg
     2581 actttactta ttgttacagc aaaggggtgt tcaagcgtgt tctaccagct gaagagtatg
     2641 cggaaattcg ctgggatcaa ctggaagcgg gtgacgttaa cttcattaaa gatttttact
     2701 accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tataccatcg
     2761 cagaacgctg gaatttgggt gaagagtact ggggctacgc gaaaaataaa ctctggtcgc
     2821 cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt tccattgtta
     2881 actgtatgtt caaccgtgac tgcatgacgc atacccatat caactttatt ggttccggct
     2941 tgccattgaa actgcaacgt gaagtggcga aagaactttt tggttctgaa gatgcttacg
     3001 atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat taaatatgct aagtggtcgc
     3061 tgttgcgggt ctgtttgcat aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg
     3121 tttcgccgct gaaaagccgt aattaccggg gcgatctggc gcttgaagcc aaattcttca
     3181 aagcgatcac tggcgaagat atgactcagg aaaaattaga tttagttgca gagcgtatct
     3241 ttacgttgca tcgcgcctac acggtaaaac tgatgcaaac taaagatatg cgtaatgaac
     3301 acgatcttat ctgttcctgg gtattcgaca aggatccgca gaacccggtc tttactgaag
     3361 gtactgacaa aatggatcgt gacgatatgc atgcttcgct cacgatgttc tacaaagaaa
     3421 tgggctggga cccacagctt ggttgtccga cccgtgaaac attgcagcgt ctggggctgg
     3481 aagatattgc cgccgatctg gcagcacaca atctactgcc tgcgtaagga atgggtgaga
     3541 tgaaccatca ggatgaattg ccgttagcga aagtcagcga agttgatgag gcgaagcggc
     3601 aatggttgca ggggatgcgt caccccgttg atacggtaac cgaaccagaa cctgctgaaa
     3661 tcctggcaga gttcattcgt cagcattctg cggctgggca gctggtggcg cgcgccgtgt
     3721 ttctctcgcc gccctattcg gttgcagaag aggaactgtc agttttgctg gaaaacatta
     3781 agcagaacgg agactacgcg gatatcgctt gtttgacggg aagccaggat gattactatt
     3841 actcaacgca ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg
     3901 acatttgtcg cgctattgca cacgccgttc gttttgagtg ccagacgtat cctcgtcctt
     3961 acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag attgaagccg
     4021 cgatcgccgc gatggatgtt gcacctgaat atgccgatat acggcaggtg gagtcatcga
     4081 cggcggtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggcg tatggcttat
     4141 gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcagggag atgtcatgga
     4201 tagggtggac agtagcagcc actgcattag gggataatca gatgtccttc actcgacgca
     4261 aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag
     4321 cgaacacgag gcaagaaaag gaagttcggt atgccatgat tcatgacgag tcacggtgca
     4381 atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa
     4441 gccgtttatc gatagcacac attccagtca ccgataacga caacgaaacg cagtatcact
     4501 tttttcgcca gtcatgtcag cactgcgaag atgctccgtg catcgacgtt tgtccgacag
     4561 cggcatcgtg gcgtgatgag caggggatcg tacgggtaga aaaatcgcag tgcatcggct
     4621 gcagttattg cattggcgct tgtccttacc aggttcgcta ccttaaccct gtcactaaag
     4681 tggcagataa atgtgatttc tgcgctgagt cccgactggc aaaaggcttt ccgcccattt
     4741 gcgtcagcgc ctgtccggaa cacgcattga tctttggtcg cgaagatagc ccagaaattc
     4801 aggcgtggtt gcaggaaaat aaatactatc aatatcagct acctggtgcc gggaaaccgc
     4861 atctgtatcg ccggttcggt caacatttga ttaaaaagga aaatgtatga acctgtcgca
     4921 acatgctgaa cagtttcaga gccagttggc gaactatgtg ccactgttca ctccccaatt
     4981 ttggccggtg tggttgatca ttgccggact attgctggta gggatgtggt tggtgctggg
     5041 gctgcatgcc ttgcttcgtg ctcgtggcgt gaagaaatca gccaccgacc atggtgagaa
     5101 ggtttatctt tacagcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt
     5161 attgttgctg gcaagtgggc tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa
     5221 aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctggcatgct ggctcggctt
     5281 tgttctgatc aatgccgttg ggggtaatgg tcaccactat cgcattcgtc gtcaggggtg
     5341 gctggagcga gcggcaaaac aaacgcgatt ctatttgttt ggcattatgc agggggaaga
     5401 acatcctttc ccggcaacaa cccagtctaa atttaatccc ttacagcagg ttgcctatgt
     5461 tggtgtcatg tatggattgc tgccgttgtt actattgacg gggctgctgt gtctctatcc
     5521 gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc
     5581 tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg
     5641 tacgccacac gaaaccttta aaagtatggt cgatggctat caccggcact aacgaatgat
     5701 cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggtttct
     5761 gcgccatttc ccacgtggcg gtagttatgc cgatattcat catgcactga ttgaggaagg
     5821 atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga
     5881 aaacttcgcg catcaggaag tttcttctat gcaaaaactg gccacagatc ccggagaacg
     5941 actattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga
     6001 taacgccaga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt
     6061 gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgcac gcgcacgggt
     6121 tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagcagtc ggattgcgaa
     6181 taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg
     6241 tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaact gcggcgacaa
     6301 tgtacacatc attgccagcg gtgaaaattc aacagtagtg agcacgggcg tggtcgactc
     6361 aattattctc gggccagggg gatgcgccgc actcgcatat catgacggcg aaagagttcg
     6421 cttcgccgtc gccattgaag gtgaaaacaa tattcgagcc ggagtccgct atcgtcttaa
     6481 tgaacaacat cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgccg
     6541 acgcgcacgg gatgcaggtt ttacgaccgc ccgagccatc gcctctccaa tttctgcaca
     6601 tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc
     6661 tacgcgccca cgaatatctt cgggttgctg taacgtataa tcttcgccaa catcagggat
     6721 ttcatcccct gttttctgta attgttcgcg tagtccaggg aaaggaatat ctttcacttt
     6781 aagcggacgc tgcccgcggg catcaataaa aacgtcaaac gagtaactgt tgtcttccgt
     6841 tttcaggacg gtgcgcgact cgttaatttc catttcgtag tcttcaccga gagcgagaat
     6901 atgaatgatt cccgcttcac gtaaggccaa tagtcgacga atagactctg aagggattgc
     6961 cgcataatta tcgatgaaaa cccgggcaag gcctttactg aaccgtttat ggtcgtgttc
     7021 attcagatgt ggaacaattt cctgtacggc ttcatgcagg cgcagaatga cataacgcca
     7081 gggaacagta tgattttctc gtttattgcg ttcgacctcc tggagatttt tttctgccca
     7141 gtcgaatggg tcgcgttgtt tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt
     7201 caggctttct aaggctatgc gttggctcca gtctggatca gcaaatttga tttcctctac
     7261 tatcaatcta aatacccgat ccaaaaggcc ctcttcgcct ttttgaatct cagcatttaa
     7321 tgcttgatca gtgacgatgt gtaagggctc gtagggaata gggcaataga aatcggcttc
     7381 gggtaaaata cctgtgcgcg acattaacgt aatatttagc ttttcacttg cgttatcgcg
     7441 gtgaaaaatg acgtgttgtt tatcatcttc aatgaacgaa ccatgctgaa tagccactgc
     7501 cattgccgca tccagtccgc tcaaggatgt tcccataata cccacgttac acgcatcgac
     7561 ctttgcttcc atcaggcctg accacgggct gggaaaatac gttctggttg cttcttcttc
     7621 atcaggccag acgtgccccg ttgcgatcac cactaaatca aacgtctcgc tgggtaaatc
     7681 cagatttgta gcgagcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga
     7741 ttcataaaca gccactgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg
     7801 atcgcggaaa tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc
     7861 ttttttaacg ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata
     7921 cgtgcaataa atcggcggta tttcaatact ggcaatgttt gccagcatca gttttgagtt
     7981 ttcatcatca ctgtatggca tcccgacacc ggcttcgtca gcctgctcga agatagaaat
     8041 agaaagtggc gtttgttgct gtagaagcga gaataaggtg tagatccctg taggcccggc
     8101 acccacaata gcaattgttt tcattgttac ttacctgtac gcattctttc gaatttagtg
     8161 ttatcaggta agtttggttg agatttttag aatttgagaa atttcagaca gggatgaaaa
     8221 gggcactaac cgcccttgat taaaatctgt caggcaagtt ttgcctgatt gatttgactc
     8281 agtggcacat tgacatcgaa aactttggcg acaacttcct caacgccaag atttttcagt
     8341 cgggcagtat gtttttccag ataggtaagg gcgctttttt catcagagaa aaggtagatc
     8401 ccaccggctt cgtggttctt ttcactttct gtccaaacct tccacagaaa accaggttcc
     8461 tgattaatcg actcagcaag tggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca
     8521 ttaaaagcaa aatgaagttg taacaaagtt gccatagcga ttctccttct gataacgcat
     8581 acgaagacag tgtagatctt ttaacgtatt ggatacatag aagaaataga gggaaaaaag
     8641 gagaagacca gattaagaaa atctggtgcg tccgagtgga ctcgaaccac cgacccccac
     8701 catgtcaagg tggtgctcta accaactgag ctacggacgc aggatggtgc gttcaattgg
     8761 actcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga gctatgaacg
     8821 caacgttgta ggtgacaacg gggacgaata ttagcggcag agtgggaagg tggcaagagg
     8881 caaaacgtat ttttctgcgc tatttcgacc gtttgcagag cttttaagca aattggctat
     8941 attttgttga tttgcaaggg cgatttttat tcaggatcgc atttacatct gacacaaccc
     9001 aataatctgt gtatcagagt ttaaacagag ttgtttgcca ccatctcaaa atctgtcgtg
     9061 gataaatcct ccgtagcctt tacacttagg tagcaataaa ggcacacaga tgcagcaaag
     9121 gaataaatca tgggatctga tgcgaaaaac ttgatgagcg acgggaacgt gcaaattgtt
     9181 aagaccggcg aggtcattgg cgcgacgcaa cttactgaag gtgagttaat agttgaagct
     9241 ggcggaagag ccgaaaatac cgtggtcacg ggggctggct ggttgaaagt ggcaaccggt
     9301 gggatcgcca aatgcacaca gtacggtaac aatggcacgc tatcggtcag cgacggtgcc
     9361 attgccacag atattgttca gtccgaggga ggcgcaatta gtctctctac gctcgctacg
     9421 gttaatggcc gccatcccga aggtgaattc agcgttgatc agggttatgc ctgcggtttg
     9481 ttgctggaaa atggcggtaa cctgcgtgta ctggaaggac atcgcgcgga aaaaattatt
     9541 ctcgatcaag agggtggcct gttggtcaat gggacaacct cagcggtcgt ggtagatgaa
     9601 ggtggtgaat tgttggtgta tccaggtggg gaagccagca attgtgagat taatcagggc
     9661 ggcgttttta tgctggcggg gaaagccaat gatacgttgc ttgctggtgg caccatgaat
     9721 aatctcggtg gtgaagactc tgacactatt gttgagaatg gagccatcta tcgtctgggt
     9781 acggatggtc ttcagctcta cagttccggt aagacgcaaa acctgtccgt taatgtgggt
     9841 ggtcgggctg aagtgcatgc cggtacgctg gaaaatgcgg taatacaagg tgggacagtg
     9901 atcctgttgt cacccaccag cgcggacgaa aattttgtcg tagaggaaga tcgcgcaccg
     9961 gttgaactga ccgggagtgt tgcattactg gacggcgctt caatgattat tggctatggc
    10021 gcagatctgc aacaatcaac gattactgta cagcagggcg gtgtattgat tctcgacggc
    10081 agtacggtaa aaggtgacag tgtcactttc agtgttggta acatcaatct caatggcgga
    10141 aaactgtggc taatcactgg tgcggcaacg catgtgcaac tgaaagtgaa acgcctgcgc
    10201 ggagagggag cgatttgcct gcaaaccagt gcgaaagaaa tttcacctga cttcatcaat
    10261 gtgaaagggg aagttactgg tgatatacac gttgagataa cagatgccag tcggcaaact
    10321 ctgtgtaacg cactgaaact acagccagac gaagacggga ttggcgcaac gctccagcct
    10381 gcgtaaaaac acaggcgcaa cccgtgaagg ttgcgccttg tctttactag cgagctgctc
    10441 gttgcagaat gatggctgac ggcagacgtt gcaggaaccg catacgcagc atcatcataa
    10501 tggctgccga cgtcaggcca ataataaagc ctatccagaa gcctgctggc cccataggtt
    10561 caacgaccag atcggtcagt gccagaatat agccgcttgg caagcccagc acccagtaag
    10621 ccgtaaaggt gatatagaaa atggaacgtg tatctttata accacgcaaa atcccactgc
    10681 caatcacctg gatagagtca gaaatctgat ataccgccgc cagcaacatc aaatgcgcag
    10741 ccagcgttac aacctcggga ttgtcgttgt acaacagggc gatttgctcc cgcagtgaaa
    10801 ccgtgaaaat ggccgtcagg gttgccatac agacacccac cataagcccg gtccgcgcag
    10861 cggtttgcgc atccagcgtt gagccctgac ccagacgata acctacgcgg atagttaccg
    10921 cagccgccag cgacatggga agcacaaaca tcagtgaact aaagttcagg gcaatctggt
    10981 gtcctgcgac atcaacaata ccgagcggag acactaacag agccacgacg gcaaacagcg
    11041 ttacttcaaa gaacagtgcc agcgcaatcg gcaaaccgag ttgaatcagt cgtttcataa
    11101 ccacaggatc gggcttagcg gtgccttttt cgttacgaat atcgcgcatg gagcgggcgc
    11161 gtttaatgta agaaaccatg gcaaggaaca tgacccaata caccgccgca gtagccacgc
    11221 cacaaccaac gccaccgagc tcaggcatac cgaaatgacc ataaataaag atatagttca
    11281 ccggaatgtt caccagcagg ccgataaagc ccattaccat acccggcttg gtttttgcca
    11341 gaccttcaca ttggttacgg gcaacctgga agaacagata gcccggcgcg ccccacaaca
    11401 acgcacgcag ataacccacg gctttgtccg ccagagccgg atcgatgttt tgcatggaac
    11461 cgataatgta acccgcattc cacagcacca gcataatgag aacggaaaca aaacccgcca
    11521 gccagaaacc ttgtcgcacc tgatgcgcaa tgcgctcacg tcgaccggaa ccatttaatt
    11581 gcgcgataac cggcgttaat gccagcagca gtccgtgacc aaagaggatc gccggaagcc
    11641 agatagaagt accgatagcg accgccgcca tgtcggtggc actatagccg cccgccatca
    11701 cggtatcgac aaaacccatc gcagtttggg cgatttgcgc gagaatcacc gggattgcta
    11761 atgctaataa cagacgcgct tcactgatat acttctgcac gtgaacacct tttatttgta
    11821 gttatatgag agactaaaaa gccgcctgaa tgggcagcaa aaagaagaag caggggaaat
    11881 tcagtctatt gtagcgagga tttattaatt ctccagtgaa aaaatagtca ttcttacccc
    11941 ttcactggta agggcgtttg ccacctgcta ttgtgctgaa cagaatgtct taactgattt
    12001 caggagttgt aagtatgttt acggggattg tacagggcac cgcaaaactg gtgtcgattg
    12061 acgaaaaacc aaattttcgc acgcatgtgg tggagttacc cgaccacatg ctggacggtc
    12121 tggaaaccgg tgcttccgtg gcgcataacg gttgttgcct gaccgtgacg gaaattaacg
    12181 gcaaccatgt cagttttgac ctgatgaaag aaacgttacg cattactaat cttggcgatt
    12241 taaaagtggg ggattgggta aacgttgagc gtgcggcgaa attcagcgat gaaattggcg
    12301 gacacttaat gtcaggtcat attatgacca ccgctgaagt ggcgaaaata ttaacctcgg
    12361 aaaataatcg tcagatctgg tttaaagtcc aggatagtca gttgatgaaa tatatcctgt
    12421 acaaaggatt tattggcatc gacggtatta gcctgaccgt cggcgaagtc acgccaacgc
    12481 gtttttgcgt ccatttaatt ccggaaacac tcgaacgcac gactcttggg aagaaaaaac
    12541 tcggcgcacg cgtcaacatt gaaatcgatc cacaaactca ggcagtggta gatacggtag
    12601 aacgtgtact ggcggcacga gaaaatgcca tgaatcaacc aggtacacaa gcctgatctg
    12661 aaatcgcccc cggagagggg gcgatagaat agcctttagc gagcgactcg aaggccgttt
    12721 tcaacaccgc gtgagaacac gacctgccag agctgaatat cacgggcgcg gaaagcacct
    12781 gcacaggcat tcagataata ggtaaacatc cgtttaaagc gttcactgta gttatccgca
    12841 atttctggcc atgcggcgag gaatcgttca taccacgcca tcaacgtagt atcgtaatca
    12901 gcaccgaagt tatgccagtc ttccatcaca aagtggggtt cgctggactg agcaatctgg
    12961 cgtacagagg gcaggcaacc gttcggaaaa atatatttat taatccaggg atcaacattc
    13021 agatcggttt ttttcgaacc gatggtatgg agcaggaata taccttccgg tttcaaatta
    13081 cgatccacca ccgcaaaata ggtatcgtaa tttttcggtc cgacgtgttc gaacatcccc
    13141 acagaaacaa tacggtcaaa ctggtcgttc aggtcacgat aatcttgcag caaaatggtg
    13201 acatccaggc cttcacagcg ttcctgagcc attttttgct gttcggcaga aatggtgacg
    13261 ccgaccacgc ttacgtcata attagacgcc atgtagtgcg ccagtccacc ccagccacag
    13321 ccaatatcca gtacgcgcat ccctggtttt aactgcaatt tttcacaaat cattttgagc
    13381 ttcgcctgct gggcagattc cagattatcg gcatctttcc agtaagcaca ggaatattgc
    13441 atgaagggat caagcatgcg gctgaacaag tcattaccca aatcgtaatg ctctttgccg
    13501 actatccagg cacgtttttt actctgcaga ttgaagagac gagcgccggc aatacgcagc
    13561 gtgtctttga aatgatgggg gagttggttc tcgagacctg cgcgtaagac tttgctaaaa
    13621 aacatatcca gtcggtcaca ttcccaccag ccatccatat aactttcgcc taaccccaac
    13681 gatccttcct gcaggacgcg tttaaaaaaa tcggggtttt tgacacgaat atccgccggg
    13741 gcagaaccgt taatggctat accggcacgg ctaagtaatt cgttggcgat acggtaccag
    13801 ttgtcatccg gtacactgac ttcttctata cacgatgaac tcatagtttc tccatcactg
    13861 gtcggtgatc agaaccttaa aacagcgtag acgctttttg ggctttgtga gaaatctcgc
    13921 agaaaaaacc gcgagtctat aatccgacgt agaacagagg gaaaggagga acccttgccg
    13981 aaatgaccat ccatagtgag tcgggaacga tcctgtcccc gtaataacat aatatttatc
    14041 gtagttttaa caactcgaaa ttagtatagg ccgctaattt caatcataca acaaaatttt
    14101 gttagcagcc taagtataag tatatcgata tagatcagtg tgattcgcta tgagcgactt
    14161 cggcattgcc atgattctgg cagccaactt cttcacaacg ttgcatcatg taacccagag
    14221 cgaccagcac tactgttgat aacatcacgc tggtggtggt gagcaatggc gtactgatac
    14281 tgataagcca ggaaactacc agacttgcga ggaagcacag acccagttgc agagtgttct
    14341 gcaacgctgc ggcgcgaccg gttgcatgtg ggaagggacg cagcgcctgg gcaacaacaa
    14401 tcgggtagat cgcgccgttg gcaatcgcca tcacacagaa tgggatcagg atttcgacca
    14461 gcgacacatg gctaatgaag cccgcagccc aggtcgcaat gacgctgaca gcaaacagta
    14521 ccagcaacca cggtaataac tgcttgcctt gccatttctg cagcgcggcg cgacagccat
    14581 aaccaccaat cagaaacgcg atagtttgcg ggacgtaact taaaccaata actgccggac
    14641 tgtaacccat ttcactaagg atgaacggtg aaccggtcag ccatgcaaaa aaactggctg
    14701 aacaggccgc gtatatcagc acgttgccgc gataggtttt agaacgtagc aggtcggtaa
    14761 aggtcagacc atcctgacta ttgttacggg ccttcgtcgt gggtttgagc cagaaaatag
    14821 gcagaatcag caccacggta atggcaaaca gggtggcgaa aatcgcctgc caggaaaaat
    14881 ggaccagcag ccagcttcct aacagaggag ccagtgccgg agacagaccc accagcggca
    14941 tgatggtcgc aaaaatacgg ttaactttct gtgaaggata ataatctgtc actaatgctt
    15001 gccagataac cgccgcggcg cagacaccca cagcctgtac aaaacgcaat atcagcagcg
    15061 tggcggcgtt ttctacccac agcatcccca gactacctaa cgcaaaaatt gtcaggccga
    15121 tgaataacac cggtttacga ccgtaacggt cggagagtgg cccccacaga agctgggctg
    15181 cggcaaaacc ggcaaggaac agactaaggc tggcactgac agcagacgca ggcgtttgca
    15241 ggtcggcttg tatggcggcg aaagcaggca gatacatatc ggttgccaga aaaccgagta
    15301 cgctcaaacc tgccagccag actaaaaatc ttttcccagg ttgcatgtat attctctaaa
    15361 tctctcaatt aaacaccgcc gcaaagtgta gggagtgaaa ttagtgttgt gaaacggtaa
    15421 tatttgatga ttggtttcaa aaattttgca ggcagactat gtggtcagaa tattctctgg
    15481 aagttgttga tgcggtagcg cgtaatggta gttttagcgc cgcggcccag gagttgcatc
    15541 gcgttccttc tgcggtcagc tataccgtgc gtcagctgga agagtggctg gcggtgccgc
    15601 tttttgaacg acgtcaccgt gatgtggaac tgaccgctgc cggggcgtgg tttctcaaag
    15661 aaggacgctc tgttgtcaaa aaaatgcaga tcacccgcca gcaatgtcag cagatagcga
    15721 acggctggcg cggtcagtta gctatcgcag tggataatat tgtcaggcca gagcgaacac
    15781 ggcagatgat cgttgatttt tatcgccatt ttgatgatgt cgaacttctt gtctttcagg
    15841 aagtgttcaa cggtgtctgg gatgcacttt ccgacgggcg cgtggaactg gcgattggcg
    15901 caacacgcgc gattccggta ggcggtcgtt attccttccg ggatatgggg atgttaagct
    15961 ggagttgcgt tgttgccagc caccacccgc tggcgttgat ggatggcccg ttcagcgatg
    16021 atacgttgcg caactggccg tcattggtgc gcgaagacac ctcgcgaacg ctacctaaac
    16081 gtattacctg gttgctggat aaccaaaaaa gacttgtcgt gcctgactgg gaatcatcgg
    16141 caacctgtat ctcggcagga ttatgcatag ggatggtgcc aacacatttc gccaaaccgt
    16201 ggcttaatga ggggaagtgg gtagcactag aactggaaaa ccccttcccg gattcggcat
    16261 gttgtctgac atggcagcaa aatgatatgt cgcctgcgtt aacctggttg cttgagtatc
    16321 tcggcgatag tgagacgctg aataaggagt ggctgcggga gccggaagag actcccgcaa
    16381 cgggtgatta acgacgatag tcgcggaacg ggccgtcagc cacggagcgg cgttcaatca
    16441 agcgcggatg cacttcaatg gactgcggtt cttcgcgttt attgacgata cgatccaaca
    16501 gcatgttgaa cgccgtttcg cccagcgaat cttttggctg atggatcgtg gtcagcgctg
    16561 gcgtaaaata gcgggcgttg cgcacgttat cataaccgat cagcgaaaca tcctgcggca
    16621 cgcgcagacc catttcatca gcagcacaaa gtgcgcccat tgccatgata tcgccaccac
    16681 agaagacggc agtagggcga tgcggctgcg acagtatttg ctgcatggcg cgataaccgg
    16741 attcaggttc aaaatcaccc tgtacaatcc agctttccgg caccttgatc atcgcttctt
    16801 ccatcgcctt cataaaaccg gcaaggcggc ctgcgccggt gttacgttcc agcgggccag
    16861 ggataacgcc gatttcgcgg tgaccgcgtt caatcagata acgcccggcc atgtaaccgc
    16921 cttcgaacgc gttatcaatg accgcatcgg tgaagtcagc ttttgcttca ccccagtcca
    16981 tcaccaccat tgggatatgg cgatactctt ccagcatcgc cagcaacggc tctgggtact
    17041 cagaacacat caccagcaga ccatcgacgc gtttttgcgc catcatcgac agataagccc
    17101 gctgtttctc aagattgttc cacgcattgc ccagaatcag ggtgtaacct ttctggaagc
    17161 aatttttttc gaccgcttca atgatctcgg caaaataggc cgcttcgctg ctggtcgcca
    17221 gcaaaccgat agacttggtg tggttaacct tcaggctacg cgccaccgcg ctaggggagt
    17281 agtgtaattc tttgattgct gcccacacgg cattgcgcgt ttcttcagcg acgaaacgtg
    17341 ttttgttgat cacgtgtgac acagttgtag tggaaacgtt tgctcgtttc gctacatctt
    17401 ttattgttgc cattccattt cactccagac cctaacttca gctcctgcaa attcgcaagg
    17461 taaacgtttg cctttacaca ccttacgcac aacaaaaatg ttgcggtacg ccggaaaaac
    17521 gacacggagc gtcaggaagg ggtcaatggc cagtacgcaa ataatgtaag cgtggaattt
    17581 tggccgatct tgacgaaaag gggaagtagt aaaaacactt atcattataa atgacgggag
    17641 attttttcat cacagtgtgt aaaaatgcgt aatatcactt tttgtgtggt aataaaaaag
    17701 gagaaaactt gatgagcacc gaccttaaat tttcactggt aacaacgatt atcgtcctcg
    17761 gtttgatcgt ggccgtgggt ttgactgccg cgctgcactg atttcctctc gagggagcgc
    17821 cttctccctc gcatctcctt cctgtttgtt agcaaaatgg caaaagtgtt ttctcttttt
    17881 gttattttca tttttttgtg attactgtca tgctttcgcc actttaatga atatcgcgta
    17941 atgacgctaa gaggtggaga taaggcatga aaattaacta tccgttgctg gcgctggcga
    18001 ttggcgcgtt tggtatcggg acaacggagt tctcgccaat gggcttgttg cccgtcattg
    18061 cgcgcggtgt ggatgtctcg attcccgctg ccggaatgtt aatcagtgct tatgcagttg
    18121 gcgtaatggt tggcgcgccg ctgatgacgc ttctactttc tcatcgtgcc cgccgcagtg
    18181 cgttgatttt cctgatggcg attttcacgc ttggcaacgt actttccgcc atcgcgccgg
    18241 attatatgac cctgatgctt tcacgcattt tgaccagcct gaatcacggg gcattttttg
    18301 gtttgggttc agtcgtggcc gcaagcgtag tgccaaaaca taaacaggct agcgcagttg
    18361 ccactatgtt tatggggtta accctggcaa atatcggtgg cgtgccggcg gcgacctggt
    18421 tgggcgaaac catcggctgg cggatgtcat ttctggcaac agcggggctg ggcgtgattt
    18481 caatggtaag tctgttcttc tcattaccta aaggtggcgc aggggcgcga cctgaagtga
    18541 aaaaagagct ggcggtatta atgcgtccgc aggtgctgtc tgcattgctg acgacggtac
    18601 tgggcgctgg tgcaatgttt accctctaca cctatatctc tccggtactg caaagtatta
    18661 cccacgtaac gccggtgttc gtcacggcaa tgctggtgct gattggtgtc ggattttcta
    18721 tcggtaacta tctcggcggc aaactggcag atcgttcagt taacggcacg ttgaaaggct
    18781 ttttgttgtt gctgatggtg attatgctgg taatcccgtt cctggcccgc aatgagttcg
    18841 gcgcagctat gagcatggta gtgtggggag ctgcaacctt tgcggtcgta ccgccgttac
    18901 agatgcgcgt gatgcgtgtc gccagtgaag cgccaggtct gtcttcatca gtcaatattg
    18961 gtgcctttaa tcttggaaat gcgctgggag cagctgctgg tggtgcggta atttccgctg
    19021 ggctgggata cagttttgtg ccggtgatgg gggcgattgt cgcgggactg gcattattgc
    19081 tggtgtttat gtcagtcaga aaacaacctg aaacagtttg cgttgccaac agctaattaa
    19141 acaaaaacgc agaaggataa ccttctgcgt tacagttgct ggcaacgtgc gatttatctt
    19201 gccgaatgcg ctgttaacgc ctcatccgtg ttggcatggc gcgcgaagcg catcaggtaa
    19261 tgttgcattt gccatcagtt attatgcagc gagatttttc gccacgaatt cccagttcac
    19321 cagcgcccag aagtgctcca gatagccagg acgtgcattg cgatagtcga tgtaataagc
    19381 gtgttcccag acatcaacgg tcagcagcgg agtcgcatcg gtggtcagtg gagtacccgc
    19441 gttagaggtt gaaacgatag ccagtttgcc atcgctgttt ttcaccagcc aggtccagcc
    19501 agaaccaaag tttttgatcg ctgcatcagt aaactgagct ttgaaatcgg caaagctgcc
    19561 aaaagatgcg gcgatagctt cagcgacttt tccagtcggt tcgccacccg cgttcggtgc
    19621 caggcagttc cagtagaaag tatggttcca gacctgagct gcgttgttga atacgccacc
    19681 ttcagagctg cgaataatct cttccagtga tttaccttca aacgcggtac ctttgatcag
    19741 gttgttcagg ttagtgacat aagtctgatg atgcttgccg tagtgatact cgatggtttc
    19801 cgcagaaatg tgcggtgcca gagcatcttt agcatatggt agtgcaggta attcgaatga
    19861 cattgctact ctcctttatt attaatttgc atacgtacaa tagccttatt gtgcgtatgg
    19921 atagggtagc aaattaaaag gtgagacaaa agatgaactt accctgttgc cgtaacaaca
    19981 gggtaaagtt ttagcgaagt gtttttgggg tcattacccg gcgagcgcca acatagtggc
    20041 gctgccagta gtcttcactg agagaagtga tttgaatttc ctgacccgta cgcggtgact
    20101 gaataaattt gccgttgccg acatacacgc cgacatgatc ggctgtaccg cgtccctgag
    20161 tacggaagaa gaccaggtcg ccgtttttca gttcactacg ttcgattggc gctgcatcgc
    20221 gcaggtgata catttcattc gccgtacgcg gaatacgaat tttcaccaaa tctttataag
    20281 cgtaataaac cagaccgctg caatcaaaac cggtacgcgg tgaactgcca ccccaacgat
    20341 atggcttacc aatttgctgc atcagtttat tcattgccac tttcgtggct ttttgcacct
    20401 tcgctttgtg cgcgtcggca agggttcctg acgcggcatt tttgactttc acacaatgcg
    20461 atttatagcc cttacgtttg gtacactttt cggtaaagtt ggctgaggcc gttttattga
    20521 cggtgtgttt gctgcgagtt ttggcgtttt tagaagcggt aatggaagat tttttaacgg
    20581 aagaagcggt tttgcttttg gtcgtacttt tactggcggc ttttttcgct gttttctggg
    20641 tctttttggt ggttgccgtg cttttctttt tatctgcttt tttggtgata tgagtagtag
    20701 cagaactctc cctggcttgc tttgaagcat gggccatagg cgttaaaggc agggtggtaa
    20761 aaagtaaagc acagagcgtg atcgaaatac ggtttatccg cgccactgag cgtccctctg
    20821 ttaaggcagg tctatcatgc ctgcgtctga tttacaaaac ttgatgattc taatgcatag
    20881 aagaagagaa agttaatagc ttttattgtt aaaaaatacg tttcgtcggc gagcgcaaaa
    20941 aaatgcagca gtgaaagcta aaaatggtta taaatacgcc agtttgccgt gcatcaggga
    21001 agaaacgaat atttgaaatg caacaaatag agcaaaggga taaaatagca aaagcgtgat
    21061 gaaaatgtta ttttccgatg ccgcactgac ccgctacaat gtcagattac tgccgtaaag
    21121 aagtttgagg aagcaagaca atgagcacca ctatcgaaaa aatccaacgc cagattgctg
    21181 aaaacccgat cctgctgtac atgaaaggtt caccgaaact gccgagctgc ggtttctctg
    21241 cccaggcagt ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc
    21301 agaatccgga cattcgtgcg gaactgccga aatatgcgaa ctggccgacc ttcccgcaac
    21361 tgtgggttga cggcgagctg gtgggcggtt gtgatatcgt gatcgaaatg tatcagcgtg
    21421 gtgaactgca gcagctgatc aaagaaactg ccgctaaata caagtctgaa gagccggacg
    21481 cggaataatc cgtcctgcaa tactgacgga tgcgaacggg catccgtcag tcctttctct
    21541 atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcaggtaac cgcgtaaaca
    21601 tcggcgtttg ccggaccggt aggtcattca cttctgttag cgtaaagcgc agccgtggtt
    21661 cgcgactgga cagctgtcgg ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt
    21721 gaaaacacgt ccggcagggg cgatgaaggg tttggattat ttgccattaa cgctattccc
    21781 cgtgaatatc atgcatgatg cgtggaatag cctcactata gatgcctgaa tcattgcatg
    21841 tcatcaggca tcgactcgat tacacctctg cggcggcagg tagtggccag cctcccagac
    21901 gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg
    21961 cggagtgcgc ctgggtgctg tcgaagtcca tgccagctgt ctggcaagcc tttgacaata
    22021 cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga
    22081 acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg tgatcgaaat
    22141 tggcgttgtg tgccaccata atggcgcggt tacaaccgct cgctttaata cctttacgta
    22201 caactttaaa aatttcgtgc agcgcctcgt actcgctgac cgcgccgcga tcgggatcgt
    22261 ttgggtcaat gccgttgaag gcaagggcct ctggttgcaa atttgcgccg acaaatggtt
    22321 cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc attttcaggg
    22381 tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct gtttcaacat
    22441 cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt tgagcgttat
    22501 cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca aatattgctt
    22561 gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat tagttgccca
    22621 gaccgcgacc ggcgtctttc tcttcgatta attcaatttt gtaaccgtcc ggatcttcca
    22681 caaatgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc acgttacccc
    22741 cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc gcgatgtgac
    22801 cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt tcaatcactg
    22861 cttcttcggt ttccgggccg taaccaacaa acgccagtga gtatttgtat tccgggtttt
    22921 cgctggtacg cagcagtttc atgcccagca cgttggtata aaaatcgatg gagcgttgca
    22981 gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tatatcctca aaatggctaa
    23041 cagattcaaa aagttgtttt agtatagcgg cggctttgcg ccgctcgcaa tgttggatta
    23101 cagcgtcggg taatcagtgt aaccttcagc accgccacca taaaaacttt cggcacgctg
    23161 cgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg caatccagtc
    23221 acgaccaaat gctaccgcat caattaaccc tttgccgatc agcgtttcag ctttttctac
    23281 tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt tttcgcgaaa
    23341 cgcatcagta tatggttcac ctcccgccca atctggttct gacatatgaa gataagcaat
    23401 gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat tcgggccgtt
    23461 atccgtgttc tggaaagtac cgattggcga aatgcgaatg ccaatgcgat cggcacccca
    23521 ttcttcaatc ccggcatcga ccacttccag caccagacgc gcgcgatttt ccacgctgcc
    23581 gccgtactga tcggtacgat ggtttgaaga aggggaaagg aactgatgca gcaaataacc
    23641 gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag caatggcctg
    23701 acggaaatca ttgacgatac ccggaatctc tcccaattca agcgcacgcg gcatggatgt
    23761 ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag
    23821 tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc
    23881 ggtgtgccac agctgtacgg ccatatgacc attttcagca tgaacgccag cggtaatttt
    23941 tttccatgcg gcaatttgtt ccgggctatg gatgccaggt gcacctgcgt atccttttgc
    24001 ctgggcagaa atttgcgtgg cttcgctaat aatcaaaccg gcgctggcac gttggcgata
    24061 gtattccgcc atcaacgggg tagggatgtc acccggttcg atactgcgca gacgcgtcag
    24121 cggtgccata aaaatacgat ttgccgccgt gatcgcgccc actttcagtg gggaatacag
    24181 tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct gctaaacggc
    24241 aggcgtcgca ataatgtttt ttacatgggc cagcgcgttt tccagtggct cgaaactgcg
    24301 cgaaattttg gcctgcagat tcgcgccaag ccacagtgcg taaagcactt gtgcctgttg
    24361 cagcggttcg ccacaaaagg ttaaacaatg gttctcacgg ccattttcca gcgcctgaga
    24421 gagcagggcg atcacgccgc gagccccttt atccattgcg ctgcgcatat cttctgacag
    24481 atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc catgttggca
    24541 aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc
    24601 ggattgcagc aactcagtca gtcgctggtg atatgcggcg taatggcgct caagcatagc
    24661 aacgccaaac gcttctttag aacgaaagta gtgatagaag gaccctttcg gcacttcagc
    24721 ggtttttagg agttcgctta gccccatccc ggtgaatcca cgttgcaggc aaagttgctc
    24781 gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca tgcgttggag
    24841 tgtagtagac cagtcggtct agtgcaagca aagttgctac tttggacatt aacagcttca
    24901 ataatggtaa taatcttatc tgaggagggc gaggtgacgg agcaattaga gttctttccc
    24961 gtccagagcc cgtgccgggg aatttgccag tctgacgaac ggggattttg tcgcggctgt
    25021 tttcgtagcc gggatgagcg ttttaactgg aataagatga gcgatggcga aaaacaagaa
    25081 gtgttgcgcc tttgtcggca gcgattaatg cgcaaattac gtgtaaataa gtcagcgcca
    25141 tcagacgaac cggagcaacc atcactcttt tgagcctaat cttgcgtata ctcctctcat
    25201 atctgataag aggaagcaat tatggttcag cgtattacta ttgcgccgca aggcccggag
    25261 ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaatatgtc cgcccgccag
    25321 ttggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga ccatgctgat
    25381 atttatggtg gctatcagtg cgaagcggcg tttggcgagg cgctgaaact ggcacctcac
    25441 ctgcgtgaac ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc gtgtgaagaa
    25501 aacgtcattg gccattacat cactgaccgc gatcacatca ttaagagcgc cgaacagtcg
    25561 ctaattaatc tcgcgaccga tcatctggat ctgctgttaa tccaccgacc agacccgtta
    25621 atggatgccg atgaagtggc ggacgccttt aaacatctgc atcagagcgg caaagtgcgt
    25681 cattttggtg tatcaaactt tacacctgcg caatttgcgc tgttgcaatc acgcctgcca
    25741 tttactcttg ccactaatca ggtggaaata tccccggtgc atcagccgtt actgctggat
    25801 ggcacgctcg accaactgca acaactgcgt attcgtccga tggcgtggtc ctgccttggt
    25861 ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact ggctgtggtg
    25921 gcagaggagt taaacgcggg ctcgattgaa caggtagttt acgcctgggt actacgttta
    25981 ccgtcgcagc cgctgccaat tatcggctca ggtaaaattg agcgcgtacg ggcagcagtc
    26041 gaagcagaaa cactgaaaat gacccgtcaa caatggttcc gtatccgtaa agcggcacta
    26101 ggttacgacg taccgtaagc acttttagga atagctgccg ttcaaaaatg tgtcactggt
    26161 ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt agtctggcca
    26221 ttctggcgct ggttgttgcg accggcgcac aagctgccag tgaaaaagtc gagatgaacc
    26281 tcgtcacgtc gcaaggggtt gggcagtcaa ttggtagcgt caccattact gaaaccgata
    26341 aaggtctgga gttttcgccc gatctgaaag cattacctcc cggtgaacat ggcttccata
    26401 ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc gccgcagaat
    26461 ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg
    26521 ggcatttagg cgatctgcct gcactggtag tcaataatga cggcaaggcg accgatgccg
    26581 tcatcgcgcc tcgtctgaaa tcactggatg aagtcaaaga caaagcgctg atggtccacg
    26641 ttggcggcga taatatgtcc gatcaaccca aaccgctggg cggtggcggt gaacgctatg
    26701 cctgtggtgt aattaagtaa ttaagtaatt aagaagccag cgtaccttgc ggcggtgctt
    26761 gctcaagttg cgaaagcgag cagtacagcc gccagacaat tgccgccagc tctcgggcgg
    26821 caggttgatg atgacgggca aggctgtcgc aaatccgctg taattcttca agtgtggccg
    26881 ccagtgattt ttgctgaacg ccacgctcac tcatcactcc ccgcaacagt gaaatacagt
    26941 tatcccgtac tcgcgataac ggatcggaac gcgattccca gtcgcgcaat tgccagacaa
    27001 catgagaaca gttcagcaac actacacccc agcgtaataa ccaacggcga gccagcgcat
    27061 cctggctgtt actcaactga ctgacgtgat gataagtgag cgattcaaat tcgctttcac
    27121 tcagtgttgg atggcggctt agttgatcga caaaatcccg gcgcagcgcg cgaatatggc
    27181 ggcggctttt acgagcatcc gatcctggac gcagaatggc gaacgctaac cacgccaacg
    27241 cgacgccaac aatttttgcc agattatcgt taagaaaatc agcaaaatca tacaccgggg
    27301 gattagtgac agcgataaac gaacccataa aaacaatcag ttgcccccac agtgcggcaa
    27361 atttggacat ctgcaattta agaagctgca tcgtcgccag cagtggaaag agaaacagta
    27421 aaaattgcca cagatcgcta atctgcacca tcaggccaaa tttgaccaca aagctgaata
    27481 gcgaaagtaa caccagcgtg cgcatcagca gtgacaacga cttaaacggt gccgcgacgg
    27541 cggagtagag tacgcagcta attgctgcca gcgttaaagc attggcaccg gcatcccatt
    27601 gcgaagcaat actccatgcg ccaatcatca ttaacgtaca aaatgtacgc agcccgctcc
    27661 acatagcttc ggcgttatcg gtatgacgag ccaggccgga tgtccgtggg agtctggcat
    27721 gatcatctac accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc
    27781 aaaaataacg tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc
    27841 gtagcgggga gataatacgt gcgacggtgt aaacatctgt ttgcgaactg gcgagcgccg
    27901 tcagcagctg ttcgagaatt tctcgcgtgg catctggcgg tgagggccag ttgagcaaca
    27961 tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa
    28021 ggcgcgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat
    28081 tcatggtcag tatctgccca atcacccctt catgtgctgc acgaatggca tcggttgttt
    28141 caggccgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcgg
    28201 ttaaaagggc tgtagcatcg gaactgctcg gcaggatcat catcatcatg ccgccgcaca
    28261 aaataccgac tatcacctcg caaacacgcg cctgagcgat atcccacagc tggctggcct
    28321 cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg
    28381 catacgcgac gttattcgtg aagtgcgcac aggcccaggt acaaaagcca agccacgccg
    28441 acatgctcaa tagaaaaaac cacggctcat tgagcgtatg tccggcaaga agcagtgccg
    28501 caatggctcc gagcaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa
    28561 cggtgggaaa gctaaccacc gcagccgagg tcatcgccca atagggctca tcaagattca
    28621 aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc
    28681 attgcgccag cgtggccctg aaccagggca aattgcgcaa ggaccatgac gatgcgttca
    28741 ttattgacct acggcaatgc tacaggtggt gcccgacacc agcgtgacat cgcctgggac
    28801 tttatccagc gtaaagtgga cgggaacgcg ttgggcgagg cgaacccagg ggacgttagg
    28861 tttcacatcc ggaataaggc ttgagtcact ttcaacgctc tgatcataaa tcgcccgacc
    28921 gatactggac acgtgaccct gtaacgtttt gttatcacta tagagggtaa tttgtgctgg
    28981 tgcaccttcg cggatatggc gcaacttggt ttcttcaaaa taaccaatga cgtaaaacga
    29041 gtggctgtca acaagggcaa acagcggttt cccggtgtca gcgtagtcgc cgatgcgggt
    29101 agtgagatta gtcacccatc cactcaccgg ggcgcgaatt tccgtttgtg ccagttgcca
    29161 ttgtgcctgt ttcagcgtcg cctgtgcggc atcaacgctg gcctgcatcg ctttaacatt
    29221 gaggttagcg gtatcgagtt cttcggcaga aataaaattt tgtgagagat gacgacgacg
    29281 attcgcttcg ttattggctt ttgcgaggcc agattgcgct ttcgccagct gagcctgtgc
    29341 gttcagctcg gcgatctgaa agggcgtttt atcgatcgtg agcagaagat cccccgcgtt
    29401 aaccagctgg ttgtctttta tattcagctc aacaatacgt cccgacacct gtggggtgat
    29461 agaaacctgt tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca tgtaaaaatt
    29521 ccacagccac catccggcaa gaaccgcaac taccgctaca atgattgttg agaaatactt
    29581 aattgtttta attgacatag ttaccacgca atcagtattg ccagagcaag gcaaacgcaa
    29641 atcgcaaaca gcgataaatc cattaacaag ggatgccaga tgtcaccggc gtagatccag
    29701 cctcgaagca ggcggtgtat gacaagccag atgacgaatc caaatgcgaa tgctttgaaa
    29761 aacggaggaa agtagacgga cgcaccgaac accagatctt gtaagggcaa tcctgttgca
    29821 ttgagcataa acttcacaaa gtagattcct ttacgacaga atatattgcg ttagattaat
    29881 aaatattctt taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt ctgacggtaa
    29941 ccaaatgcag caatacattt gttttagcaa tacaattgct gcacactatt ctaaaagccg
    30001 cataatatct tagcaagcta attataagga gatgaaattg gaatcgccac taggttctga
    30061 tctggcacgg ttggtgcgca tatggcgtgc tctgatagac catcgcctga aaccgctgga
    30121 gttaacacaa acccattggg ttacgttaca caatatccat cagttacctc cagaccagtc
    30181 gcaaattcaa ctggcaaaag cgattggcat cgagcagcca tcactggtcc gtacgctgga
    30241 ccaactggaa gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc gtcgggctaa
    30301 acgtattaaa ctgacggaaa aggcagagcc actgatcagc gaaatggaag ctgttattaa
    30361 caaaacccgc gcggaaattt tacatggcat ctccgcagag gaactggaac aactgattaa
    30421 gctcatcgca aaacttgagc ataatatcat tgagttacag gccaaagggt gaaatgaagg
    30481 gggcgtgtgg tcagtaacct gaccacacgc aaacttattt agcgcgggga aacggtcacc
    30541 tgactgccat tgctggccag tacgacacgt tggcctggag agaaatgagt gttcccttgt
    30601 ttctgtacca ccatgatggt gttgccatca tctttacgaa tttccagctc gacaccctgc
    30661 gttttgttca ttgcactctg tacgccctga ccggctacac cacctgcaac agcccctgct
    30721 gcagtagcca gagaacgccc ggttccgccg ccaatagtat ttcccaggaa accaccaagt
    30781 acagcaccac caattgcgcc gataacgttg gaatcatcac cgccctgaat ctgtacctga
    30841 cgtacattaa cgatggtgcc atagctgaca ttctgtactt gtttcgcttc agaagcggta
    30901 taaacatccc ctgacagggt gtcgttatta acacaaccga caagagacag acctaccatt
    30961 gaaacaacca atacgcgttt aatcattgaa aaatctcctg ttcaccatga aacgccacgc
    31021 aagcatccct catggtcaaa gtatatggca tatgtgaatc catggttaca tgttctacca
    31081 aatatgagca aatcataatg aaagtaggat caaccaatta taaacaaaat attcatgatg
    31141 attattgatt tgaagataat gttttactac caacggtaaa aattggtcat tccgcatggt
    31201 attcacgaaa agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa ggatgaacta
    31261 tgaaatcggg ccgctttatt ggcgttatgt caggcaccag ccttgatggt gttgatgttg
    31321 tgttggcgac aattgatgaa caccgggtcg cacagctggc aagtttgagc tggccgatcc
    31381 cggtgtctct gaaacaggct gtactggata tttgccaggg ccagcagctt acactttcgc
    31441 agtttggaca gcttgatact caactcgggc gactttttgc tgatgcggtc aatgccttgc
    31501 ttaaggaaca aaacctgcag gcgagagata tcgttgcgat cggctgtcac ggtcaaaccg
    31561 tctggcacga accgacgggc gtggcaccac acactttaca gattggcgat aacaatcaaa
    31621 ttgtggcgcg caccggaatt acggttgtcg gtgattttcg ccgtcgcgat attgccttgg
    31681 gaggacaagg tgcaccgctg gtacctgcgt tccatcatgc gctgctggct cacccaaccg
    31741 agcgacgaat ggtgctcaat attggcggca tcgccaatct gtcactgctc attcctggcc
    31801 agccggttgg gggctacgat accggtcctg gtaacatgct gatggatgcc tggatctggc
    31861 gtcaggccgg taaaccttac gataaagatg ccgagtgggc acgggcgggt aaagttattc
    31921 tcccactgct gcaaaatatg ctcagcgacc catatttctc gcaacccgca ccgaaaagca
    31981 ccggacgcga atactttaac tacggctggc tggagcgcca tttgcgccat tttccggggg
    32041 ttgatccccg tgatgtgcag gccacagtgg cagaactcac cgccgtgacc atttctgaac
    32101 aagttttgtt gagcggtggc tgcgaacgat tgatggtatg tggtggaggt agtcgtaatc
    32161 cgctactcat ggcgcgtctg gcggcattac tgccaggcac agaagtcacc accaccgatg
    32221 ccgttggcat tagtggcgat gacatggaag cattggcgtt cgcctggctt gcctggcgaa
    32281 cgctggcggg attatcagga aatctgcctt ccgtcactgg cgcaagccag gagacggtac
    32341 tgggggctat tttccccgct aacccgtgat atcagagtta actgaatttt tcttaccgcg
    32401 tctgccgtta cacttggaac gttagggagg gcgtattgcc ctccagacca ggaaagtctt
    32461 cgggatattt ctatgaaaaa actgttactg atctgtttgc cggtgcttct caccggctgt
    32521 agcgcgttca atcaactggt tgagcgcatg caaaccgata cgctggaata ccagtgtgat
    32581 gaaaaaccgt tgacggtcaa actgaataat ccgcgccagg aagtcagttt tgtttacgat
    32641 aatcaactac tgcatctcaa acagggcatt tcagcgtctg gtgcacgtta caccgacgga
    32701 atctatgttt tctggtcgaa aggcgaggaa gcgactgtct ataaacgcga ccgcatcgtc
    32761 ttgaataact gtcagttaca aaatccgcag cgttgagatt tttccagggg cggcgcacaa
    32821 tagcgtcacc cactgacaat ccgtaaagaa aaccatgtct gataacgacg aattgcagca
    32881 aatcgcgcat ctgcgccgtg aatacaccaa aggcgggtta cgccgccgcg atctacccgc
    32941 cgatccatta accctttttg aacgttggct ctctcaggct tgtgaagcca aactggctga
    33001 ccctaccgcg atggtggtcg ctaccgtgga tgaacatggt cagccttatc agcgcatcgt
    33061 tttactcaaa cattacgacg aaaaaggcat ggtgttttac accaacctcg gcagccgtaa
    33121 agcacatcaa atcgaaaata atccgcgcgt tagcctgctg ttcccgtggc atacccttga
    33181 acgccaggtg atggtgatcg gtaaagcgga acgactttcg actctcgaag tgatgaaata
    33241 ttttcatagc cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc agtccagtcg
    33301 catttctgcc cgcggtatcc ttgaaagtaa atttctggag ctgaagcaga agtttcaaca
    33361 gggcgaagtg ccattgccca gtttttgggg cggttttcgc gtcagccttg aacagattga
    33421 gttctggcag ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga
    33481 tgcgtggaag attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct
    33541 ggtactcctg attctggcac tttattctat gtctctttcg catctggcga aaagtcgtgt
    33601 accggcaaag gtgcagtcgt tatatacatg gagattttga tggcaagcag taacttgatt
    33661 aaacaattgc aagagcgggg gctggtagcc caggtgacgg acgaggaagc gttagcagag
    33721 cgactggcgc aaggcccgat cgcgctctat tgcggcttcg atcctaccgc tgacagcttg
    33781 catttggggc atcttgttcc attgttatgc ctgaaacgct tccagcaggc gggccacaag
    33841 ccggttgcgc tggtaggcgg cgcgacgggt ctgattggcg acccgagctt caaagctgcc
    33901 gagcgtaagc tgaacactga agaaactgtt caggagtggg tggacaaaat ccgcaagcag
    33961 gttgccccgt tcctcgattt cgactgtgga gaaaactctg ctatcgcggc gaacaactat
    34021 gactggttcg gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa acacttctcc
    34081 gttaaccaga tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga agatcagggg
    34141 atttcgttca ctgagttttc ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac
    34201 aaacagtacg gtgtggtgct gcaaatcggt ggttctgacc agtggggtaa catcacttct
    34261 ggtatcgacc tgacccgtcg tctgcatcag aatcaggtgt ttggcttgac cgttccgctg
    34321 atcaccaaag cagacggcac caaatttggt aaaactgaag gcggcgcagt ctggttggat
    34381 ccgaagaaaa ccagcccgta caaattctac cagttctgga tcaacactgc ggatgccgac
    34441 gtttaccgct tcctgaaatt cttcaccttc atgagcattg aagagatcaa cgccctggaa
    34501 gaagaagata aaaacagcgg taaagcaccg tgcgcccagt atgtgcttgc agaacaggtg
    34561 acacgtctgg tgcatggtga agaaggtttg caggcggcaa aacgtattac cgaatgcctg
    34621 ttcagcggtt ccttgagtgc gctgagtgaa gcggacttcg aacagctggc gcaggacggc
    34681 gtaccgatgg ttgagatgga aaagggcgca gacctgatgc aggcgctggt cgattctgaa
    34741 ctgcaacctt cccgtggtca ggcgcgtaaa actatcgcct ccaatgccat caccattaac
    34801 ggtgaaaaac agtccgatcc tgaatacttc tttaaagaag aagatcgtct gtttggtcgt
    34861 tttaccttac tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa ataatgcatt
    34921 aagtggaaag ggggagtgag aaatcactcc ccctggtttt tatacaggga acatgatgaa
    34981 aaatattctc gctatccagt ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga
    35041 gtttccgatg cgccgcttgg gcgcgaacgt ctggccgctg aacaccgttc aattttctaa
    35101 tcacacccaa tacggcaaat ggactggctg cgtgatgccg cccagccatt taaccgaaat
    35161 tgtgcaaggc attgccgcca ttgataaatt acacacctgt gatgccgtat taagtggcta
    35221 tttgggatcg gcggagcagg gtgaacatat cctcggtatc gttcgtcagg tgaaagcagc
    35281 gaatccacag gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa aaggctgtat
    35341 cgttgcaccg ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca gcgatatcat
    35401 tgcgccgaat ctggttgagc tggaaatact ctgtgagcat ccggtaaata acgtcgaaga
    35461 agcggttctg gcagcgcgcg aactcattgc gcaagggcca caaattgtgt tggttaaaca
    35521 cctggcgcga gctggctaca gccgtgaccg ttttgaaatg ctgctagtca ccgccgatga
    35581 agcctggcat atcagccgtc cgctggtgga ttttggtatg cgccagccgg taggtgttgg
    35641 tgatgtgacg agcggcttac tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc
    35701 cctggaacat gtgaccgctg cagtatacga aatcatggtg accacaaaag caatgcagga
    35761 atatgagctg caagtggttg ctgctcagga tcgtattgcc aatccagaac attatttcag
    35821 cgcaacaaag ctctgaaata tctcatttcg gcccacaacg acctgtgggc cgatggctat
    35881 tactttaagc cttccgctga cagcgcgtct tgtacttccg gacgttcagc catacgttgc
    35941 ataaatgccg caatgtgctc taacccttcc agattcagtt tcaccgcgta tgcccagcgc
    36001 agaaccgtaa acagataggc atcagcaatt gtaaatcgtt gcccgcagat ccagtgctca
    36061 tccttcagtg cctcgttcac atattgcagc ttcttctcca gctgcgcgcg aactgtcgat
    36121 ttgtactctt ccggtgtatc agggcgaaac agaggtgtga aacctttatg cagctcggtg
    36181 gcgatgtaat tcagccattc gatggtttta tagcgggaaa tactgtttac cggtgccagc
    36241 aactggcggt cggggacgct gtcggcaaga tactgcataa tcgctacgcc ttccgtcagc
    36301 aaagtaccat cgtccagcag caatgcaggt acctgtccct tagggttaac ggcaaagtaa
    36361 tcgtcaccgt tttcgagacg ttttttcatt aaatccacac tgacgagcgt aaaatccttt
    36421 ccgctctcac gcagggtgat atgggaagcg agagagcagg cgcccggttt gtagaacaat
    36481 ttcatcggta actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa
    36541 aaaagccgct aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctacg
    36601 gctgctttcg ccgctttgtc tgcagcgtca tcctgcgtca tgcggtgcag tttcggcgcg
    36661 gtaaacagca tcagtactgc aataacggca gtagcgacac caatctgcaa gaatacgcga
    36721 ccatagactt ccagtgacat cagcggatcg gtaacgttat ccggcacagc catcatacct
    36781 gcaacataac caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg
    36841 aagcccatca gacgctgcgg aacgagttgt gcaaccattg ccagacccag gccagagatc
    36901 atcagttccc cgatactctg caggccatag ctcgcgacca gccagcttac agacacgata
    36961 ccagcgtcag acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc
    37021 accatgccga tagcaaactt ggttggcatt ggcagggtat cgcccatctt gttatagata
    37081 gcggccagaa tcgggctacc aatgatgatc cagaacgggt tcagtgcctg atactgctca
    37141 ggttctacgg ccaggcccag aatggtgtgt tcaacgttac gaatcgcaaa gaagttcagt
    37201 gacgttggca tctggctata aagcacgaag aagataatgg cttcgagcat caggatgaag
    37261 gcaacgatca ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata
    37321 accacgatac cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc
    37381 agccaggtgg cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg
    37441 tagttgatag gctcgaagtc tggttttgaa ccgtactgtt taacccaacg ttggcagaag
    37501 gcgaagttaa cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag
    37561 ccgtatttcg cggcaagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg
    37621 gacatgtagt acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta
    37681 gaaagcagag aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata
    37741 cccatataaa cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc
    37801 agcacaatag cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag
    37861 ccgccgatag cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa
    37921 tccgcttcag acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg
    37981 taataaccaa aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt
    38041 ttaaaagcgt tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc
    38101 tcttttttta catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac
    38161 gcgcaattgt tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag
    38221 acaagtcctt tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg
    38281 aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactaccctg
    38341 agagggtgat gatggcttaa tggcgatatg ttctactatt tggcgttttt cctagtgaga
    38401 tagtccagtt tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt
    38461 gtaaatttaa atgattttaa cctgattctt gcaatgtttt tacattgcca cagcatcgtg
    38521 atcttgatca cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt
    38581 ggttatctgc aaacggatta cctgatgata acgctaagca atggcatcat tgacgttaaa
    38641 aagatgtttt aaaagaggat aaagaaaggt tatgaatggg gtaatcggtg ttacccctga
    38701 tctttagatg tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca
    38761 gcgaggcttg cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc
    38821 gactttaaac tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt
    38881 ccccggcgca aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt
    38941 cggccagccg aatgcagtgt ttaatacgac gttggctgtt ttacgaccta caccaggcag
    39001 ggcttcaagc gcagcacgat cttccggaac ctcgccatta tgttgttcca gtaatatacg
    39061 gcaggttttg atgatatttt ctgctttgct gttataaagg ccaatcgttt tgatataggt
    39121 tttcacccct tcaacgccca gttcaagcat cgctgcaggc gtattagcca ccgggtagag
    39181 tttcgccgtc gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat
    39241 cagcaattca aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg
    39301 caggcgagtg aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg
    39361 tttggcaatg cacgttcggc agctgcttca gtacggcgct ttttcatttt ttcatcaatc
    39421 aggtattttc ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc
    39481 agcaggaaag gggagtcggt gtggaaaatc tccacgcgta atactttcgc ccagctacct
    39541 aacagcgcat ctgcaccgtc aaacaacgtg ccattgccga taatttcgcg tagtgaaccc
    39601 agcacgaaca tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc
    39661 gccggacctt ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca
    39721 atcagcggaa taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc
    39781 atctgtacag cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg
    39841 gcgggtgtcc agtgacgcag cgtcgaaatg gtcaggttgg ttagcgtcag taccagcgta
    39901 gtcgcaagtc ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa
    39961 aggccgagca gctggaccag cgcagagttg tttttccaca acccctgaac aataacgtct
    40021 ttaatttcgc tcacgtttta ttctccacag gcaggaagtt gagaaagttg tgcgggtaac
    40081 gtctgagcgt acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc
    40141 gcgccggtga actggtcgaa atcaccacca tctttcttca ccgcccagtt cgcatcatcc
    40201 gcaccactga tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt
    40261 ttatcgccaa gccctggcgt ttcgtggtgc tcagtcacgc gcgtgccaag taccgtgccg
    40321 ttaaaatcgg ctcctaccag cagctgaatc gcacctgaat agccatctgg cgcggttgct
    40381 tccagaacgg cagctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct
    40441 ttacctaact ctggcgcagt gaccagatag caactctgtg ccagcgcatt gttatagcgt
    40501 tcagctggca gcacctgatc aaataacgcc ttttgttgca gactggcctg ttcagcaatt
    40561 gtcgttttgg tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaagagc
    40621 gccaacgtaa tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatgaccg
    40681 tagacgcgcg gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg
    40741 gcaaaagcca tgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc
    40801 gccagcgcgc cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc
    40861 aaaataaaga atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc
    40921 agagtatctg gtgagaacaa ccagcccaac gttgcgcata acgccaacgt tgctaagaag
    40981 ctgaggggaa tatgccagcg aatcgctttc tgccatagca accacacgcc gccagccagc
    41041 caggcgagat ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat
    41101 tgcataatct cttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt
    41161 gtcgcctgac taatgccatc aatacctaag cgtagcgtgt tcatatcacc accactggtg
    41221 gtatgaccac tgaaaataac ctgtatggtg tcgataaaac cagggatgtt gaccgcaatt
    41281 tcatgtggcg gtaaccaact ggtcatctgc accgggaagg agatcagtaa gaccacataa
    41341 ccaatcattg ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg
    41401 atcaccgcaa acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt
    41461 accgccagca ataagcctgt cagcaatgct gagttatctt tcaacgttgc agctaccgac
    41521 tgcttgcgta gtttgaggac gagagcttcg gccagaagag cactgaccga cgccaacagg
    41581 atctgaacga gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct
    41641 gcgagcaata ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag
    41701 ctagctattc tgaataccat ttagtcctcg tttacaacct tttgctgggc ggcttttttg
    41761 gcctgaacgc gggcaatagc cgcagcaact gccgctttgc gcggatcaat ctgttcttct
    41821 ggctctgcat tagcctgttg ctgttccagc ttgcgcgctt tggcgcgtgc aatagccgct
    41881 tcgatggcgg ctttgcgtgg atcaacctgt tcttctggtt ccgcattagc ctgttgttgc
    41941 tgttccagct tgcgcgcttt ggcacgggca atagccgctt cgacggcagc tttgcgcgga
    42001 tcaatctgtt cttctggctc cgcattggcc tgttgctgtt ccagcttgcg tgctttggca
    42061 cgggcgatag cggcttcgac ggcggctttg cgcggatcga cctgttgttc tggctccgca
    42121 ttggcttgtt gctgttccag cttgcgtgct ttggcgcggg caatagccgc ttcaactgca
    42181 gttttacgtg gatcagcaac ggttgcagcg tcgttagttt gctgcagttc tgcctgtttc
    42241 gctctggctt gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt
    42301 tcgcccgctt taatcacaat aggctgtgta gcctgggcct gtttctcttt cacccgcgcc
    42361 agggcggcag caatcgcatc tttatctttg gctgcaggtt gaacggctgc gctcttatgt
    42421 cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct
    42481 ttggcttctg cggcgcgctt ttcttcctga cgaatagccg caatttcagc tttttcctga
    42541 cggaaatatt gcaccagggg gatattgctc gggcaaaccc acgcgcaagc tccacattca
    42601 atgcagtcgg caatgttatg cgtggtggct ttatcgtgtt gctgaccttt gctgaaccag
    42661 tacagctgtt gcggcaaaag atccgcaggg caggcgtcag cacaggcgct acaacggatg
    42721 caactttgtt cttcctgtgg ttcgccaagt tcattggcag agggagccaa cagacagttg
    42781 gtaatcttta cgaccgggac atccagccac ggcaaggtaa agcccattag cgggccgccc
    42841 ataatcacca tttggtcggc agaggggcag aatccggcgt cattcaataa atgacgcact
    42901 ggcgtcccca accgtgccca gacgttgccc gggcgagcga ttgcttcgcc agtcagggtc
    42961 acgacacgct cggtaatcgg ctcgccatca ataacggcac gtttcactgc ataagcggtg
    43021 ccgacgtttt gcattaatac gccgatatcg gatgaacgcc cgccatgtgg aacctgcttc
    43081 ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag gatatttggt gggaatcacc
    43141 cgcagagaaa tatcgtgaga gtctgccagc accgcgcgca gcatggaaat cgcctgcggt
    43201 ttgttatctt caataccgat aagaatttcg cgtggctgca gaatatgcgc aaggatgcgg
    43261 ataccctcta cgacctgagc cgcacaatcc tgcatcaaac ggtcatcggc ggtaatgtac
    43321 ggctcgcact cagcagcgtt gataatcaac gtttcaatct tatctccgcc gccctgcaat
    43381 ttaacgcctg tcggaaatcc cgcaccaccc agcccggcaa caccaaactg atgaatgcgc
    43441 tcgattaact cttcgcgact gcgagagcga taatcggccc agccgtcgcg cgggatccag
    43501 cagtcttcgc catcggcatc aataatcaca cttaattcag ctaaagctga aggatgagcc
    43561 gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac cggcagcatt
    43621 ttgccgcgac cacgggtaag cggctgaccg cgcaatacgt tatcgccgac gctaacgcac
    43681 aactcacctt cagcgccaat atgttgtttc agtggaatga caaaacgctg cgccagtggt
    43741 acctggcgca ggggcgtacc gttggactgg gttttcatct ccggtggatg gatgccgccg
    43801 ttgaaatccc agattttatt ttttctgaat gcagagaata acttaagcat ggtgttccac
    43861 gggaatgata cgcacgggaa tggtgttcag atcccatttc caggagtcag gagtttctgc
    43921 gacaggttgc aacgagatgc agtgtgtcgg gcacggatca acacataaat tgcagcccgt
    43981 acagagatca ctcattaccg tatgcatggc gcgggtagcg ccaacgatgg cgtctaccgg
    44041 acacgcctga atacatttag tgcagccaat acagttattt tcatcaatca ccgccaccat
    44101 ccgcgctggc gttagctctt gcgcttcgcc atccagcggc tgcggctcga cattaagcaa
    44161 ctcggcaatt tttagcatca cagcttcgcc acctggggcg caacggttga ttttttcacc
    44221 gttacagctg atggcttccg cgtaggggcg acagccggga taaccgcact gaccacactg
    44281 gctctgcggt aagatttcgt caattttctc aacgaccgga tcgtcttcca ccgcaaaacg
    44341 gcgggaggca taacccagaa tggcgccaaa cgccaggccc agcaggctca cggcggcaac
    44401 ggcaatccag atagcattca ttacaacttc accaaaccac taaagcccat aaaggccaga
    44461 gacataagac ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc cgggacatca
    44521 gccacagcaa ggcgttcgcg gatggcggcg aagagcacca tcaccagcga gaaaccgaca
    44581 gcggcggaaa aaccgtacag cgctgactgc aagaaattgt gcccgagatt gatattcagc
    44641 aacgccacgc cgagcacggc acagttggtg gtgataagcg gcaaaaaaat ccccagcaga
    44701 cggtaaagta ccgggctggt tttgcgcacc accatttcgg tgaactgcac aaccacagca
    44761 atcaccagaa taaacgccag ggtgcgcagg taaatcagat taagtgggat caaaatccac
    44821 gtatcgataa gccaggcgca aatagacgcc agcgtcatta caaacgttgt tgccagcccc
    44881 atgcccatcg cggtttccag ctttttggaa acccccataa acggacagag accgagaaac
    44941 ttgaccagta caaagttatt gaccagtaca gttccgacaa acagtaacag gtagtcagtc
    45001 atttttcggc ctgaaataaa aaagccgcct attatccgtt aatccagagc aggcgacaac
    45061 aggttaactg taaggttatt acggattcac aaacgtcgct ttcacccgcg acgagcgttt
    45121 gaaatagggg acgatcaatg cggttgccaa caaagtaaac agcaactggc gaacggctat
    45181 gccatcttct acgggtgaaa aagcgaacgc ttttactgcc agcaatacgg aaataagcag
    45241 ccagataata tagtgtttag gaacacaacg ccgacgttta aagaaagcga tggtcaacca
    45301 cagtgtgtaa taccacatgg cgatagcggt aataaaagaa acgccccata agatttgtgt
    45361 cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg cagcggtata
    45421 cagcaacaac gccagcgtcg tactcaacag agcgactaaa agccaggcca acgggccaag
    45481 caaccagcct ccaatacgtt gtggcgttgt ggtggtcata ggttctccag aatagcgacg
    45541 caaacagaca aatctaattt taggggcgtt attataaggc ttttataacc gatcgctact
    45601 ttctttactg gacatagcgc cagacggact tcggtacctg gccgaggtca aacaaacgcc
    45661 cgccagaaac cagctctgcg cgacggtgat cggcagcacg atacatattg atcagttcct
    45721 gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt ttccagactt tcgagtgaac
    45781 tgattttgcg aaattttaat aagtagtcct gaacagtcat aaaaggtcca taaaataatt
    45841 aataccaaag gggtaggtat tattagtgat gataaaattt caccaaaagt gaacgcttgt
    45901 gttattacaa taataagaat gtgctgatgg caaatattca gggatgaaaa actgctctgg
    45961 aaagagtaac gcgctttact atccctgaca atcatcaaca acatcgaaca agataataaa
    46021 ttcctggttt aatatccgac aagtgaaaac atgcacccgg acgggcagca tgtcgctcaa
    46081 caagtgcaga gcttacttgt gttgtactgt agtactctgt tcaggtggct gatagttgtc
    46141 aatgtgactc gccacgccaa gaagaatgac tgaaacgaca agaacgatcc aacctgttaa
    46201 ttcaataaga cgattcatta cagcccacat ctctgttgat tgatccatta acttcagggg
    46261 gtaaatgtta cttagcaata atagctccag caagattttt actgaggttt ttgcaatatt
    46321 tagcttttgt cgttggaaaa atcgctattt ttttgactta agttaaacaa catcccttat
    46381 tgctggcagg ttattaaact gttgagcgag ggtaaaggat agtgtcaaat agccatcata
    46441 cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt
    46501 atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg gagctggcgg
    46561 taatctccag cagcgatgaa acaaaagtaa aagctgactg gccaacggtt acggttgtct
    46621 ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca
    46681 acgataccca ttttccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg
    46741 ataaaccctt taccgtgaca ctgtcacaag cacgagagct ggaagcgctg gcaaaaagcc
    46801 tggggcgagt gctgtctgta ttccataacc gtcgctggga tagcgatttc ctgacgctaa
    46861 aaggtttgct cgcggaaggc gtgctgggtg aaattgctta ctttgagtcc cattttgacc
    46921 gcttccgccc gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct
    46981 ggtacgattt agcaccgcac cttctggatc aggccattac gttatttggt ttaccggtta
    47041 gcatgacggt agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg
    47101 ctatcttgtc ctatccgcag cggcgagtga ttttacacgg caccatgctg gcagcagccg
    47161 agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc
    47221 cacaggaaga acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata
    47281 tgcgcgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga
    47341 cagtgccggg gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg
    47401 gtgaaaatcc ggttccggca agtcaggcaa tccaggtaat ggagttgatt gagctgggca
    47461 tcgaatccgc caaacatcgc gcgactttgt gccttgcgtg aaaaggggat ggtgcaatta
    47521 gcaccatcca tttttgttac ttcgcggcga ctttttctcg cagtgcgcgt ttttcctctg
    47581 cgctgaggaa agccatttcc agaccattaa tctgtgcctg gcggatttgc tcgcgggata
    47641 acccagcagc tggcgcggca acggtatatt cgtgaatgat atccactccc tgcacgcctg
    47701 gatcgtcagt gttaatgctg gcacgaatgc catgctcaag gaacgttttc agcggatgtg
    47761 cagccagatc cgccacggtg ctggtctgaa tattggaggt cagacaggat tcaataccaa
    47821 tttgttgctc ggcgagaaaa tccatcagcg cccgatcttc aatggctttt acgccatgtc
    47881 caatacgctc cgcccccagt tcacgaatcg cctgccagat gctttccggt ccggcagctt
    47941 cgcctgcatg gacggtaata tgccagcccg catcacgcgc gcggttgaaa tgagaaagga
    48001 acagacttcc cgggaaacca agttcatcac cggctaaatc aagtgcggta atctggtcac
    48061 ggtgggctaa aaaggcctcc agctcttgct gacaggcggc ttcgccgaag gtccggctca
    48121 taataccgat aagcttcgcc tgcacaccaa aggtgcggca accttcacgt acgccatcga
    48181 tcaccgcttc gacaacaccc gctacaggca gctgatgtgc cattgccatg tagcctggtg
    48241 aaaaacgcag ctcgacatag tgcaggccgt tacgggctgc atcttcaatg ttttcaaatg
    48301 ccacgcggcg gcaggcatca agagaggcga gaactttaac gccccagtca agtttagtca
    48361 gaaagctcac cagatcgggt tcgttggcaa tgacctgaac gtggggaatc agtgtttcca
    48421 gggattgtgc aggaagcgag atattatact ggcggccaag ttcaagaatg gtctggggac
    48481 gaatgttgcc atcaaggtgg cgatggatat cagttaatgg cagggtggta tcaatcatgg
    48541 tcgcactctt tttctggata taaaagtgcg caccattata aaaacaaagc agggtaaaaa
    48601 tccacgattg cgcaacgatt ttttacgttg cgcaattggg gttaaccaac agcgcggatg
    48661 gcgttaatta atccagccac acctttttcc agtttcgaac gtgggcagcc ggcattgagt
    48721 cggataaaac cacgaccttc ttcaccgtag gtatatcccg gcatgatcgc gatcttttct
    48781 tgctcgataa gcgctttttg caacgcgttg tcgtcaatat tcaacggacg tagatcaagc
    48841 caggccagat aagtggattg cgggatctgc cagttgagtt caggaaacgc agcgttcatt
    48901 ttatctgcga tatacgtcag gttatctttc agatagacgc gtaaggcatc cagccatggc
    48961 gcgccttgct gataggcggc gatatgagca gttaacgcca gtaccgaagg ggaagaaagc
    49021 ccatcacgtc ctttcagtgc cgataaatag gcatcgcggc tactgctatt ttctataatc
    49081 ccgtaagcac cggtcagggc gggaatattg aaactttttg atcccgacgt tagcaacgcc
    49141 cagtctccgc gcgccacatt actccaggga atatgcggct gctcgcccca aaccatatcc
    49201 atatggattt catcggaaat aacccgcaca ccatgacgct cgcacaggtc agccatgatc
    49261 tccagctcat cgcacgtcca cactttcccg gtaggattct gtgggctaca caggagcaga
    49321 attttgcatt ctggtttcgc caacacggct tccagcttgc ccatatcgca aaaccagccg
    49381 tcaggctgct tctctaaagc aacgggcatt actgtgcgct ggttaccttc aatggccttg
    49441 taaaatgcgt cataggcggg tgtgtggatc accacgcctt cacctgtttc agaccactga
    49501 cgaatcagtt ctgaaaccat atagataaca gaagggccat acaccaccga ctgaggatcg
    49561 atggcggtgt aatgctgggt ggaaaaccag tgggcaatag ccgcgagaaa ctcatcgttt
    49621 ttccagcggc tgtagccaaa cacgccgtgt atcaggcgct gattcagcgc ctcgataatg
    49681 cagggggcag tggcaaaatc catgtctgaa atcgtgaacg gtaacaggtc agcagtgccg
    49741 aaacggtcag cgacataatc ccactgcgta caccatgtgc catgacgatc cacgaccttt
    49801 gaaaaatcga acatatctta tccttatgcc tggacagtat gcatcagacc ggccatttca
    49861 tcttttactg actgaacttg tggcccgata acaacctgca ggttatgttg attaagttgt
    49921 actacgccaa ttgcccgatt gtccttcagt gcctgcacat taacaagcga catatctttt
    49981 acagacaaac gcagacgcgt aatgcagtta tcgaggctga caatattgtc ggcaccgcct
    50041 aaggcttcga ggattgccgg aacgttgtaa cctgatttac ccggcgcacc ggcaacggct
    50101 ttttcgattg aactggcaac ttcactatcg cgccccgggg ttttgagatt gaagcgggtg
    50161 atagcgaaac ggaagatgat gtagtaaacg acaaaccaga ttgccgccac tactggcacc
    50221 atgtaccact tggttgacag accatgcaaa ataccgaaca ccacaaagtc gatgatattg
    50281 ccgtcggtat taccgatggt gacgccgagc acagacatga cagtgaagcc gaggccggtt
    50341 aacagcgcgt ggatgacata cagaactggc gctacgaaca ggaaaaggaa ttccagcggt
    50401 tcggtcgtgc cgccaacgac acaggcgatc aggccagaaa tcagcagacc tttaatttta
    50461 tggcgatttt ccgggcgcgc gcagtgatac atagctaacg ctgcacctgg cagaccgccg
    50521 agaaacgcag gcattttgcc ttgcgaaagg aaacgcgtgg cgctttcaga aaaaccgtga
    50581 gtggtcgggc aactcaattg cgcctggaag atggtcagtg caccgctgac ggtttgaccg
    50641 cagacttcct gcgtgccgcc tgcgtcggta aagcgaatta atgccaccag aatgtgatgc
    50701 agaccaaacg gcaacagcag acgttcaccg gtaccaaaca gcatcggtcc gaaatctccc
    50761 gcgctgttga tcatatggcc taagccgcta atacccatgg cgaaaatcgg ccagactaat
    50821 ggaatcacca ggccgacaag acccatcact agcgaggaga tgattggtac gaagcgcgta
    50881 ccgccgaaga atgccagcgc atccggcagg cggatattat ggaatcgctc atgcagcatc
    50941 cagacgataa taccggcgat caccgcaccg aggatcccgg tgtcgatcga ctggatccca
    51001 agaatgctct ggatgttatt ggctttcaga accgcggcgt ccgtggttgg cagaatgcct
    51061 ttattggtca accagaagtt taccgcgagg ttcattaccg cataaccgac gaagccagcg
    51121 aatgccgcta cgcctttgtt ttcgcgtgcc agacccagcg ggatggcgat acagaacatg
    51181 actggcaaga aactaaaagc aaacgagcca atcttactca tccaggtaaa gatagcctgc
    51241 aacacggggt tgcccaggac cgggatcagg gtgatgacat catggctgct aagagaacta
    51301 ccaatgccga gcataatgcc gcagaacgac aataatgcca cgggtaacat gaaggttttg
    51361 cctaactgct ggaagaactc ccacagcgtg actttcggtg ctgttttcgc cgtcataaaa
    51421 cgactcctcg tagagaacaa tctggattca ataaattgcg cgagaagata aaacgtttta
    51481 tcaaatttta gtgaggcata aatcacatta cacaacgata atagcgggta taagataaat
    51541 aaaaggtaaa acgttttatc tgtcacataa tcagggagta ggtcatctgc atggctaccg
    51601 ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg cgtgtcggta agtaccgttt
    51661 cgctggtgct tagtggcaaa gggcgaatct ctaccgccac aggagaacgc gttaacgccg
    51721 ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc ggcgctgcgc ggcgggcaaa
    51781 gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc gttttacgcc gaattgacgg
    51841 ccggattgac ggaagctctg gaagcgcagg gacggatggt ttttttgctt cacggcggta
    51901 aagacggcga gcagctggca cagcggtttt cactattact gaatcagggt gtcgatggtg
    51961 tggtcattgc cggggctgca ggaagcagcg atgacctgcg acggatagca gaagaaaaag
    52021 ctatcccggt gattttcgct tcccgtgcca gttatcttga tgacgttgat acggttcgcc
    52081 cggacaacat gcaggctgca cagttgttga cggagcatct cattcgcaat gggcatcagc
    52141 gtatcgcctg gctgggaggg caaagttcct cattaacccg ggcagaaagg gtggggggct
    52201 attgtgcgac tctactaaaa tttggcctgc cgtttcacag cgattgggtg ctggagtgca
    52261 cttccagcca gaagcaagcc gcggaagcta tcacggcgct tttacgtcat aacccgacca
    52321 tcagcgccgt ggtttgctat aacgaaacta ttgcgatggg ggcatggttt ggtttgctga
    52381 aagcaggaag gcaaagcggg gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc
    52441 tggcggcatt taccgatgca acgccaacca cacttgatga tatacccgtt acctgggcca
    52501 gcacgccagc gcgggaactt ggtaccacac ttgcggatcg catgatgcaa aaaatcaccc
    52561 atgaagagac gcattcacgc aatcttatta ttcccgcccg gctcattgcg gcgaaataat
    52621 cctctcttta tctgctatac ctggtagtgt cctttcctca aggttaatga taaaaaatgt
    52681 ggcacacagg cggtgccttt tatttttact gaagttcaca ggaggtttat gtgtttaatt
    52741 ctgacaacct gagactcgac ggaaaatgcg ccatcattac aggtgcgggt gcaggtattg
    52801 gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc tgtggtggtc agtgatatta
    52861 acgccgatgc agctaatcat gttgtagatg aaattcaaca actgggtggt caggcatttg
    52921 cctgccgctg cgatattact tccgaacagg aactctctgc actggcagac tttgccgtca
    52981 gtaagttggg taaagttgat atcctggtta acaacgccgg tggcggtggt cctaaaccgt
    53041 ttgatatgcc aatggcagat tttcgccgcg cttatgaact gaatgtattt tcttttttcc
    53101 atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg cggtggcgtt attctgacca
    53161 ttacttctat ggcggcagaa aataaaaata taaacatgac ctcctatgca tcatctaaag
    53221 ctgcggccag tcatctggtc agaaatatgg cgtttgacct gggtgaaaaa aatattcggg
    53281 tgaatggcat tgcgccgggg gcaatattaa ccgatgccct gaaatccgtt attacaccag
    53341 agattgaaca gaaaatgttg caacacacac caatcagacg tctgggccaa ccgcaagata
    53401 tagctaacgc ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc
    53461 tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgaa ccggtaaaca
    53521 gccgtatgtg ttgtttaccg ggatagcgtg tgcaactcat caacgtctct gctaaacaac
    53581 tggcagccaa atcacggcta ttggttgacc aatatctgga taaaaagctt acgaattgag
    53641 tgtgccttcg cactattcaa cagcaatgat aggcgctcac ctgacaacgc ggtaaactag
    53701 ttattcatgc taactataat ggtttaatga tggataacat gcagactgaa gcacaaccga
    53761 cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg
    53821 cctcgatgaa agccatctgt aaatcctgcg ccattagtcc cgggacgctc tatcaccatt
    53881 tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag agggcgctgg
    53941 cccgtttccg ggaaccgatt gaagggattc atttcgttga ctatatggtc gagtctattg
    54001 tctctctcac ccatgaagcc tttgggcaac gagcgctggt ggtcgaaatt atggcggaag
    54061 ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcacggaat
    54121 ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca gacatcaaca
    54181 cggcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag
    54241 cggaagacct ggctcgtgaa gcgtcgtttg ctcagggttt acgcgcgatg attggcggta
    54301 tcttaaccgc atcctgatcc tctctctctt tccgcgggct ggtgacaact gagcccgcgt
    54361 ttcatatcgt aatttctctg tgcaaaaatc atcctcccag ggatcggaga atcccaccta
    54421 aatattcatc gtagcaatat atcatgaggg tttatcgttc ccaatatgtt cgaacgaacg
    54481 ttcgattgct tattttgtgg cttctgtcaa cgctgtttta aagattaatg cgatcaatat
    54541 cacgctgtgg gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc
    54601 atttctcttc catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat
    54661 cagattaaag gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt
    54721 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
    54781 cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat
    54841 tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta atcatgtggg
    54901 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcgggcc agcgtattgt
    54961 actgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtaaataatc aggaagtgat
    55021 ggagcatcag ggcggctata cgccatttga agccgatgtc actccgtatg ttattgccgg
    55081 gaaaagtgta cgtatcactg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc
    55141 gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttactttc atgatttctt
    55201 taactacgcc gggatccatc gcagcgtaat gctctacacc acgccgaata cctgggtgga
    55261 cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcatctg ttgactggca
    55321 ggtggtggca aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc
    55381 aactggacaa ggcaccagcg ggactttgca agtagtgaat ccgcacctct ggcaaccggg
    55441 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
    55501 cctgctgcgc gtcggtatcc ggtcagtggc agtgaagggc gaacagttcc tgatcaacca
    55561 caaaccgttc tactttactg gctttggtcg tcatgaagat gcggatttgc gcggcaaagg
    55621 attcgataac gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc
    55681 ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg
    55741 catcgtggtg attgatgaaa ctgcagctgt cggctttaac ctctctttag gcattggttt
    55801 cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaaccca
    55861 gcaggcgcac ttacaggcga ttaaagagct gattgcgcgt gacaaaaacc acccaagcgt
    55921 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
    55981 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
    56041 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
    56101 gaaccgttat tacggttggt atgtccaaag cggcgatttg gaaacggcag agaaggttct
    56161 ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
    56221 cggtgtggat acgttagccg gcctgcactc aatgtacacc gacatgtgga gtgaagagta
    56281 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ctgtcgtcgg
    56341 tgagcaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
    56401 taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tctgcggctt ttctgctgca
    56461 aaaacgctgg accggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat
    56521 caacaactct cctggcgcgc catcgtcggc tacagcctcg gtgacgtcgc caataacttc
    56581 gccttcgcaa tgggggcgct cttcctgttg agttactaca ccgacgtcgc tggcgtcggt
    56641 gccgctgcgg cgggcaccat gctgttactg gtgcgggtat tcgatgcctt cgccgatgtc
    56701 tttgccggac gagtggtgga cagtgtgaat acccgatggg gaaaattccg cccgttttta
    56761 ctcttcggta ctgcgccgtt aatgatcttc agcgtgctgg tattctgggt gccgaccgac
    56821 tggagccata gcagcaaagt tgtgtatgca tatttgacct acatgggact cgggctttgc
    56881 tacagcctgg tgaatattcc ttatggttca cttgctaccg cgatgaccca acaaccacaa
    56941 tcccgcgccc gtctgggcgc ggctcgcggg attgccgctt cattgacctt tgtctgcctg
    57001 gcatttctga taggaccgag cattaagaac tccagcccgg aagagatggt gtcggtatat
    57061 catttctgga caattgtgct ggcgattgcc ggaatggtgc tttacttcat ctgcttcaaa
    57121 tcgacgcgtg agaatgtggt acgtatcgtg gcgcagccgt cattgaagat cagtctgcaa
    57181 accctgaaac ggaatcgccc gctgtttatg ttgtgcatcg gtgcgctgtg tgtgctgatt
    57241 tcgatcttcg cggtcagcgc ctcgtcgttg ttctacgtgc gctatgtgtt aaataccggg
    57301 ctgttcactg tgctggtact ggtgcaaaac ctggtcggta ctgtggcatc ggcaccgctg
    57361 gtgccgggga tggtcgcgag gatcggtaaa aagaatacct tcctgattgg cgctttgctg
    57421 ggaacctgcg gttatctgct gttcttctgg gtttccgtct ggtcactgcc ggtggcgttg
    57481 gttgcgttgg ccatcgcatc aattggccag ggcgttacca tgaccgtgat gtgggcactg
    57541 gaagctgata ccgtagaata cggtgaatac ctgaccggcg tgcgaattga agggctcacc
    57601 tattcactat tctcatttac ccgtaaatgc ggtcaggcaa tcggaggttc aattcctgcc
    57661 tttattttgg gattaagcgg atatatcgcc aatcaggcgc aaacgccgga agttattatg
    57721 ggcatccgca catcaattgc cttagtacct tgcggattta tgctactggc attcgtcatt
    57781 atctggtttt atccactcac ggataaaaaa ttcaaagaaa tcgttgttga aattgataat
    57841 cgtaaaaaag tgcagcagca attaatcaac gatatcacta gttaatattc aataaaaata
    57901 atcagaacat caaaggtgca actatgagaa aaatagtggc catggccgtt atttgcctga
    57961 cggctgcctc tggccttacc tctgcttacg cggcgcaact ggctgacgat gaagcgggac
    58021 tacgcatcag actgaaaaac gaattgcgca gggctgataa gcccagtgct ggcacgggaa
    58081 gagatattta cgcatgggta cagggaggat tgctcgattt caatagtggt tattattcca
    58141 atattattgg cgttgaaggc ggggcgtatt atgtttataa attaggtgct cgtgctgata
    58201 tgagtacccg gtggtatctt gatggtgata aaagttttgg ctttgccctg ggggcagtaa
    58261 aaataaaacc cagtgaaaat agcctgctaa aattaggtcg cttcgggacg gattatagtt
    58321 atggtagctt accttatcgt attccgttaa tggctggcag ttcgcaacgt acattacgga
    58381 ctgtttctga aggagcatta ggttattggg ctttaacgcc aaatattgat ctgtggggaa
    58441 tgtggcgttc acgagtattt ttatggaccg attcaacaac cggtattcgt gatgaaggtg
    58501 tttataacag ccagacggga aaatacgata aacatcgcgc acgttctttt ttagccgcca
    58561 gttggcatga tgataccagt cgctattctc tgggggcatc ggtacagaaa gatgtttcca
    58621 atcagataca aagtattctc gagaaaagca taccgcttga cccgaattat acgttgaaag
    58681 gggagttgct cggcttttac gcgcaacttg aaggtttaag tcgtaatacc agccagccca
    58741 atgaaacggc gttggttagt ggacaattga cctggaatgc gccgtgggga agcgtatttg
    58801 gcagtggtgg ttatttgcgc catgcaatga atggtgccgt ggtggacacc gacattggct
    58861 atcccttttc attaagtctt gatcgtaacc gtgaaggaat gcagtcctgg caattgggcg
    58921 tcaattatcg tttaactccg caatttacgc tgacatttgc accgattgtg actcgcggct
    58981 atgaatccag taaacgagat gtgcggattg aaggcgcggg tatcttaggt ggtatgaact
    59041 atcgggtcag cgaagggccg ttacaaggga tgaatttctt tcttgctgcc gataaagggc
    59101 gggaaaagcg cgatggcagt gcgctgggcg atcgcctgaa ttactgggat gtgaaaatga
    59161 gtattcagta tgactttatg ctgaagtaag aaaaaacgcc ggagagaaaa atctccggcg
    59221 ttttggctgt gaattactgc tgcggaatag cgggtacagc cggaacgttt aatgccggca
    59281 tcgcaaacat acccacgaaa tcttccagtg gcattttctg cccgtttaac gttatctgac
    59341 cgttagcata ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc agacggaaca
    59401 tctgccccat tgctgatgca ccttcaactt gctgtttcgc cagttttttc gcttgatctt
    59461 cctgataacc ttccagcttc gctacctgag tcattaactc agttgccata tccaccggaa
    59521 tggtcagttt cgcatccaga gatttaaccg accggtccac ttcctgcgcc agcgtttgcg
    59581 gcgcttcttt agtcgttgcc ggatctttca ggaacagcga cagattcagc gcactttcac
    59641 cctgactgtt tttccagctt agcggcgcga tagtaatcac cggatcgcct ttcagcatca
    59701 gcggcagagc gctaaagaag gcttccgtca ctttctcctg ataaagttcg ggattgttgg
    59761 caatttctgg ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg ctaaactgat
    59821 gccaggcttc gccatcaatt tggccgactt ttaaagtcag cttgccactg cccagatcct
    59881 gattctgtac cttcaggctg tttagcgagt aatccagttg gctattgacc gttttaccgt
    59941 cattgaccag atccgattta ccgctgatct ccatgccttc cagcagtgcc agttctttgc
    60001 cctccactga aatggtcatt ttttccagcg acagtttttg gtttcctacg cgctcaccaa
    60061 aacttgccag cgtgctggaa ccgtcggttt tcagattatt aaaggttaac tgcactttct
    60121 ggttgtattc gttaaccgcg tctatccgac cactttgcgc ctccccggaa agggagatag
    60181 ctttgccgtc tctgtctgca tttaactgga actcgccgcc gctaaatgcg actttttcat
    60241 ccttttgctc gtaattcagt ggcttgagcg aaatgtcgga actggaatct ccgctgtaac
    60301 caatgcggga gttaatctca aaaggcgttt caccttttgc catatcaaac agtggtttgc
    60361 ttacttcgtt attaaccagc gtggtttgaa ttgatgccat cgatgggatc aggttcagtt
    60421 ttttaagctg ggcaagcggg aagggaccat gatcaaccga ttcgttgaag atgacgctct
    60481 gaccgctttt aatccacgga ttttctttcc cggcaatggg ttttaccaac agttgcagct
    60541 gactgctgaa tacgccgcga tgatagtttt gataactcac ttccaggttg gattcaggag
    60601 cggtcagttt gagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg gtttcaatct
    60661 tcttgcctgt ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca atgacgccta
    60721 ccgctaccag cgatttattc ataatgatta tccataaaat gaaatcaggc ggactggccg
    60781 cctgaaggtg ttataagcct ttaataagct tagcaagaga tgttaatttt ttcagtaagc
    60841 tcttacagct tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt caccggtgat
    60901 tcgttggcgg caataaacgc tgattcaccc ggtttaagct gtaactgctg agaaccttta
    60961 cacagcgttg catcgccttc gacgcagaac aaaatggcgg cactctgctg gctgatggtg
    61021 gtttctttat cactaaggtc atgcagcgag aaggcaaaat catcaacagg aatcgggaag
    61081 tccagttctg caccttgttt caccggctgg gtcaacaact ggttagccgg tttggcttcg
    61141 aacttcacat tggcaaccag ttccggaata tcaatatatt taggcgtcag acccgcacgc
    61201 agcacgttat cggagttcgc catcacttcc agcgccacgc cttgcaggta agcgtgcggc
    61261 gtttcagcga acaggaacat cgcttcgcca ggattcaatt tcaccacatt cagcaacagc
    61321 ggggagaaca gaccgctgtc atccgggtaa aattcagaaa ttaaacgaat cgtttgccac
    61381 ggttcaccct gctggctgtc aagggccgat tttaaaatcg ccagcgcgcg ggatttttct
    61441 tccccctgca tattcagcag gctggcgaac agttcgctta aacgttcggc atcaggctgt
    61501 tgtaaaaagt gagcaatcgc cggatgtgca cctgcgactg gctggagtag ggagacaatc
    61561 tcggaaaatt cacgaaacgc gttcatcgca aggaaaggcg tcagcgcaaa aaccagctcc
    61621 ggcttgtggt taggatcttt atagttacgc tcggcggcat ccatcggaat acctgcggca
    61681 ttttctttgg caaaaccgat ttcagaattg cgtttgtttg gatgaacctg aatggagagc
    61741 ggctgtgctg cgcataatac tttgaacagg aaaggcagtt cgccaaagcg tttggcaacg
    61801 gcctctccga gcagagtcga tttatcactc tcaatcacat cacgcagtga aacgatatct
    61861 ccggcggcat tctgcactcg tgaactgctt tttggatgtg cgcccatcca cagctcggcc
    61921 atcggctggc tggacggatt ttccatacca taaagttcag tcaacgccgt tttgctgccc
    61981 caggcatagt tttgcactga gttaatgagt ttttgcatga tcaatccctg ttttaatgtg
    62041 gaaattaatc ccgctattaa agcaagaatc ctacgggaag taacctggag ccgcaaaaag
    62101 tcgtactagt ctcagttttt gttaaaaaag tgtgtaggat attgttactc gcttttaaca
    62161 gggcaacgga acacccgccc agagcataac caaaccaggc agtaagtgag agaacaatgt
    62221 caaacaaacc ctttcattat caggctcctt ttccactcaa aaaagatgat actgagtatt
    62281 acctgctaac cagcgaacac gttagcgtat ctgaatttga agggcaggag attttgaaag
    62341 tcgcacccga agcgttaact ttgttggcgc gtcaggcgtt tcatgatgcg tcatttatgc
    62401 tgcgtccggc tcaccaacaa caggtggccg acattctgcg tgacccggaa gccagcgaaa
    62461 atgataaata tgtggcgctg caattcctgc gcaactccga tatcgcggcg aaaggcgttc
    62521 tgccaacctg tcaggatacc ggcaccgcga ttattgttgg taaaaaaggg cagcgtgtat
    62581 ggaccggtgg cggtgatgaa gcggcgctgg cacgcggtgt ctataacact tatatcgaag
    62641 ataatctgcg ctactcgcaa aacgcgccgc tggatatgta taaagaggtg aataccggca
    62701 ccaacttgcc agcgcagatc gatctttatg ccgttgatgg cgacgagtac aaattcctct
    62761 gtatcgccaa aggtggcggt tcggcaaaca agacgtatct ctatcaggaa accaaagcgt
    62821 tactgacgcc gggaaaactg aaaaattacc tggttgagaa gatgcgcacg ctgggtacgg
    62881 cggcctgtcc tccgtatcat attgcgttcg ttattggtgg aacttctgca gaaacgaatc
    62941 ttaaaacggt gaaactcgct tccgctaagt actatgatga actgccaacg gaagggaatg
    63001 agcacggtca ggcgttccgc gatgtggaac tggaaaaaga attgctgatc gaagcgcaaa
    63061 atcttggtct gggtgcgcag tttggtggta aatacttcgc tcatgacatc cgcgtgattc
    63121 gcctgccacg tcacggcgca tcctgcccgg tcggtatggg cgtctcctgt tctgctgacc
    63181 gtaatatcaa agcgaagatc aaccgtcagg ggatctggat cgaaaaactg gaacataatc
    63241 caggcaaata tatcccggaa gagctgcgca aagcaggaga aggcgaagcg gtgcgcgttg
    63301 accttaaccg tccgatgaaa gagatcctcg cacagttgtc gcagtatccc gtttctacac
    63361 gcttatcgct taacggcacg attatcgtcg gtcgtgatat tgctcacgcc aaactgaaag
    63421 agcggatgga taacggtgaa gggctgccgc agtatattaa agatcatccg atttactacg
    63481 cgggtccggc caaaacgccg gaaggttatg cctccggttc tcttggccca acgaccgctg
    63541 gccggatgga ttcttatgtc gatcaattgc aagcgcaggg cggaagtatg atcatgctgg
    63601 cgaaaggtaa tcgcagccag caggtgacgg atgcttgtaa aaaacacggc ggcttctacc
    63661 tcggcagtat cggtggtccg gcagctgtat tggcgcaggg aagtattaag agcctggaat
    63721 gtgttgaata tccggaactg ggaatggaag ccatctggaa aattgaagtg gaagatttcc
    63781 cggcgtttat ccttgtggat gataaaggga atgacttctt ccagcagata caactcacac
    63841 aatgcacccg ctgtgtgaaa taaacagagc cgcccttcgg ggcggttttt ttacatggca
    63901 cgaaagacca aacgtttgtt atcaaatggt aaataatgag tgagctaaaa gttgcttagc
    63961 gaaagcaaaa cagaaagaaa aaattaatca ggtgaggagc aggccatgaa tacagtacgc
    64021 agcgaaaaag attcgatggg agcgattgat gtcccggcag ataagctgtg gggcgcacaa
    64081 actcaacgct cgctggagca tttccgcatt tcgacggaga aaatgcccac ctcactgatt
    64141 catgcgctgg cgctaaccaa gcgcgcagcg tcaaaagtta atgaagattt aggcttgttg
    64201 tctgaagaga aagcgagcgc cattcggcag gcggcggatg aagtactggc aggacagcat
    64261 gacgacgaat tcccgctggc tatctggcag accggctccg gcacgcaaag taacatgaac
    64321 atgaatgaag tgctggctaa ccgggccagt gaattactcg gcggcgtgcg cgggatggaa
    64381 cgtaaagttc accctaacga cgacgtgaac aaaagccaaa gttccaacga tgtctttccg
    64441 acggcgatgc acgttgcggc actactggcg ctgcgcaagc aactcattcc acaacttaaa
    64501 accctgaccc agacgctgag tgaaaaatcc cgcgcatttg ccgatatcgt caaaatcggt
    64561 cgtacccact tgcaggacgc gacgccgtta acgctggggc aggagatttc cggctgggta
    64621 gcgatgctgg agcataatct caaacatatc gaatacagcc tgcctcacgt agcggaactg
    64681 gctctgggcg gtacagcggt gggtactgga ctaaataccc atccggaata tgcgcgtcgc
    64741 gtagcagatg aactggcagt cattacctgt gcaccgtttg ttaccgcacc gaacaaattt
    64801 gaagcgctgg cgacctgtga tgccctggtt caggcgcacg gcgcattgaa agggttggct
    64861 gcgtcactga tgaaaattgc caatgatgtc cgctggctgg cctctggccc gcgctgcgga
    64921 attggtgaaa tctcaatccc ggaaaatgag ccgggcagct caatcatgcc ggggaaagtg
    64981 aacccgacac agtgtgaagc attaaccatg ctctgctgtc aggtgatggg gaacgacgtg
    65041 gcgatcaaca tgggtggcgc ttccggtaac tttgaactga acgtcttccg tccgatggtg
    65101 atccataact tcctgcaatc ggtgcgcttg ctggcagatg gcatggaaag tttcaacaaa
    65161 cactgcgcag tgggcattga accgaatcgt gagcgaatca atcaattact caatgaatcg
    65221 ctgatgctgg tgactgcgct taacacccac attggttatg acaaagccgc cgagatcgcc
    65281 aaaaaagcgc ataaagaagg gctgacctta aaagctgcgg cccttgcgct ggggtatctt
    65341 agcgaagccg agtttgaccg ctgggtacgg ccagaacaga tggtcggcag tatgaaagcc
    65401 gggggttaat ctgcaacata caggtgcagc cgtggaatga tcaaacgaag cggctgcgcc
    65461 tgtggtttat aacggtgctg cacattgtcg gcatcgtaat ttaacaactc accaacgtcc
    65521 ggcacgcccg cgccattatc cctattaatc agtgcaatca gcggcgtagg gcaggcgtgt
    65581 tgtacttgtt tttgatctcc tttgtaccag acgcgggcaa tcggctgcac cttcaccgga
    65641 cgtttgattt ttaacttcgc gttctgtggc agggcagcga tatcctgata ttctcgctcc
    65701 agttttcttt gccactcctc gcgcgtccac ggtgcgacac tgcgtggtga tttcaggctt
    65761 ttttccagct gtgccaggac ttcatcacga tgcaaatttt taatgatatg tttattagcc
    65821 cagccaaaac gtaaagtggc ggggtcgtgc agaacggtga gcgtgcggta agcattaagg
    65881 gtaatcagcc ccggcaaatg acgatgcacc cattcaaaac gtgccgcgga ggggagttct
    65941 gattcaaccg tgacgatatg ctcgaacgtg gtcttgagtt tattgatgtg ctgaatatga
    66001 ctgactaacg ctgcttgcga aaagttatcg acctggtaac acaacacgcc aggcagacgg
    66061 acagcggcct tgctgctgcg attttcggac tgttgttgaa taaataaatg gcgaaaatga
    66121 cgcaacgcca gcgattgcgc gtcgttgccg agatgttgtt ttatctcaat acgattaagc
    66181 ggattatgct catcctcttt ttttacctcc ggcaaagaga acacgcgggc aaccaatagc
    66241 ttgtgttgct caagatgagc ggcaaatgca gccagctctt gttccatctg gcgaaaagta
    66301 gtgttgagtc ggtctacgag atcgtaacgc gccataatat taaccacttt agttacaaca
    66361 tccttatttt atagcacaat ggtggtgact tgaccaccgt gcgcatagtg tcaggcagag
    66421 gtaaattgcg ggatgttatg ccataacggc cagctgaacg agaagcgggc accacccagt
    66481 tcgctggtgt cacaattaac cgtaccgccc attgccagtg ctatagagtg gacaatggcc
    66541 agccccagcc cgcagccgcc ggttgagcga tcccggctgg gatcgaggcg aacaaaaggt
    66601 tcaaagatat gttcgcggtt ttctggggca atccctgggc catcatcctc aacaattaat
    66661 gtcgctctat tccccgacag tagcaggctg atttcaaccg ttgagtgaca gtagcgcagg
    66721 gcgttattga gcaaattatc cagcacgcgc tccattaagc gcatatccaa cgccgcataa
    66781 tggccttgcg cgagcgtttt tatccgtacc gttttatcgg gcgttactgc ctgaatatct
    66841 gccagatgcg ttgacagcca caacggcagg tctggttcgc taagatgaag ctcattttgt
    66901 gggcggtcga gtcgggcata agtcagcagc tcttcaatta aagcttcaag ttgactgata
    66961 tcgcgattca acgcctggga ttcggcggcg ctcaggttat cgctcatctc cagtcgataa
    67021 cgcaggcgca ctaacggtgt tcgcagttcg tgagcgatac cgtcaataag ctgttttttg
    67081 ctggcaatca aggcgttgat attgtccgcc atctggttaa atgcgacgcc aagtcgttca
    67141 aagctcgaac cttcatcaaa gtggatacgc tcactgagat gcccatcgcc aaatcgttgc
    67201 gccgctgctt ccagttttaa catatcctgc cagtgcggac gcatccagat aaacaccgga
    67261 aaggcgaggg aaatggcaat aaaagcgatc agggcgatat ccagcaatcg catctgatgg
    67321 aggtaataaa gataaggaac aggaccaact gccagcacgt agtggctgcg cgggatacgt
    67381 tgcaaaaaag tgtactgatc gtccagagcg acaatttcgc cgccacgcag ccggtgcatg
    67441 gaaatatcat caagatgata tttactcagc ggctcgacac gcagatcgaa agagagattt
    67501 aaatccatct ctttcagcgt tttaccccag tcgtgtgggg ggatctcacg caattcgctg
    67561 cgcatcagat acagcgaact gttcatcaaa tcatccagcg actgtttgcc tgcgcgttcg
    67621 gcggtaaatt tgtacaccag cccgaccagc agagacatca caaggaagca gacaaacaat
    67681 aacaggtaaa actggataaa cagttttttc atcgcttatt cccacgcatg aggcgcaaaa
    67741 agatagcctt tgttacgcac ggttttaata cgataaggtt ctgcggcgtt atcgagcagt
    67801 ttttttctta accgcgaaat agccacgtcc acgctacgat ccagtccgtc ataactgacg
    67861 ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt gcccggcatg ggtagctaat
    67921 tcccacaata attcgaaatc agctgtcgag agcgagattt cagtgttagc cagggtgact
    67981 acgcggttga tgggatcgat ggtcaacgtg ccgaaatgca gggctttgta gggagtcaga
    68041 gacgtttcct gaataccttt ggtcactgtg gcttgctcat tctgacgcaa atgcaaacgt
    68101 aaacgcgcta gcaaaacagc agggggcgtc gttttgagaa tatagtcgca ggcacccatt
    68161 tccagtgcca ggatgtggtt catatcgcta tcgagagagg ttagaagaac aatcggtccg
    68221 gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt ccttgcctgg tagcatgatg
    68281 tcgagtaaca ccaaatccgg attttctcgc agaatagttt cttcggcccg gtcgccgcgc
    68341 ggctctacgg taacctgcat atcatgtttt gccaggtatg cggcaatcag tgcaccgact
    68401 tccgcatcat cttccacaaa tacgatagtg ttcataacat tcacggtaga tataaaaacg
    68461 tcacaataca ccgcactgtt tttacttact atcaatcgtt tctaaacaag gtttcatcag
    68521 gtattctact aatgacttgt tatggaagtg ttaaggtaaa aagatggggc tggtaatcaa
    68581 agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa cgaaaaatta
    68641 ttatatcgcc gggctgattc cacttttccc gacctttgcg cttatcgcgc attatattgt
    68701 tgccagcgaa cgcggcattg aagccttacg cgcaaccatc atttttagta tgtggtcgat
    68761 tattccctat tttgtttacc tggcgtccct atggtatttc accggaatga tgcgcttgcc
    68821 cgctgctttt gttggctctg ttgcgtgctg gggaataagc gcatgggtgt tgattatatg
    68881 ctggattaag ctgcattaac gcagatgacg actgccatca agtgggagac tgcgtccggt
    68941 gacaaagcag ctggtaagta agtaatcgac caggtcgatc acttctttct cgccaggcgc
    69001 ggttttcatc agtgatttat tcagcgcctg ttgtcgatat tcggcatcat catgttcatt
    69061 aaacaggatc agcgatggcg caatagaatt cactttcact tccggtgcca gcttgcgggc
    69121 aaacgagcgg gtcatattat ccagtgccgc tttgcttgca gcataggcaa tatgtttgtc
    69181 gctaccgcgc tccaccacat aatcggtaaa gtgaatgata tcgctggcgg cgtgtccgtg
    69241 cccacgcagt aatctttcca gagcatggtt gagcaggtat ggggtattaa cgtggatctg
    69301 catcatgcaa gccagtacgt cggtcagtgg cgctcccggt ttttccgcca tccacgcgct
    69361 ggcgttatgc aaaatagcac gcagaccatg ggtgcttttt agtacttcat cggcaaacgc
    69421 catcacaccg tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg ccttaatcag
    69481 tccatcaatg gtgggatagt gcgtccgata gctgacaatc accggttgct tttgattaat
    69541 gaaatgccat gcgagggcga ggccgatgcg gcgacctccg ccagtaatta atattggcaa
    69601 ggactgggtt ttacccatcg ttatctcctt tgctatccaa cgatgggatg aacgttatcc
    69661 caccagcatc caggtcgccg gaactgaggc aattaacagc atgccaatca gcaccatttc
    69721 ctgacggttc agtacattat catgtgtatg cgttttacgc gcgtacagga aaaccagtag
    69781 ccccggcgca tacagaacaa cggataacag caagtgcatt ggaccagagg cgtacagtaa
    69841 ccataagcca taaatgcagg caccgacacc taccgcctta tgcagtggac gggttgcgat
    69901 tttcagcaag aacgcgccga ccaggaaata gggcaccaga atcatttctg aggcgatggt
    69961 cagcaatgtg ttgtaatcgg aaccggtcag ccagatgagc accagacaaa tttgcacgca
    70021 gatgttggtc agccacagtg aagcggatgg cgcagcttgt gcgttctggc gtgcaaaaat
    70081 gcgcgggaat gctttatgag tggctgcaag gaacggaact tctgctgcca tgatggtcca
    70141 gcttaagtac gcgccgcaaa cggaaacgat caaaccggca gcgatgatga tttcgcccca
    70201 cggtcccatc atttccacca tcagaccggc cattgacggg ttacgaattt cagccagttc
    70261 aggacgtgcc accacgccca gcgaaagcag cgttaccagc aaataaacgc ccagagcgga
    70321 gagaaccgcc agcagtgtcg ctttgccaac atcacgctta ttacgcgcac gcgcagaaac
    70381 aaccacggca ccttccacac caatgaaaac ccacagggtg atcagcatgg tgtttttcac
    70441 ctgttcccac acgggtacgc caagtgcaag tccggtgaag tcgagcttga aggtatccag
    70501 tttgaacatc attatcgcca gcacaacaaa cagacccaac ggcaacaatt ttgccagtgt
    70561 cgccacaagg ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa agtgaacaat
    70621 ccacaataaa gccgatgcac cgacgatcga ttgccaggta ttaccatcgc caaacaggcg
    70681 caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca gataggagac
    70741 gttggcgatg accgcgcaca gccagtatcc ccatgcggaa caaaagccga ttagctcgcc
    70801 aaacccttcg cgggcatagg taaagatacc gccgtcaagt tcggggcgaa tgcgcgtgag
    70861 gatcagcatg gcaaaggcca gcaataaaat gccagcgcca gtaatacccc agccgatgag
    70921 cagtgctgcc gggctggcaa ctgccgccat attttgcggc agactgaaaa cacccgcgcc
    70981 cagcattgag cttaatacca gcgcggtgag tgcgctcagt cccagtttct tttccatcga
    71041 tatccagtga ttaaaacgtg acaagaagaa taaatattag ggcaaaacgc ataaaaatgg
    71101 agtttgccac tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa tggtcgcagg
    71161 gaaatttatg actaaaaatg cgaaaggcgg catcaagccg ccttatagga gtaactgaat
    71221 agttatttat acagatctgc gctgacggtc aggttattac cacgttcctg ccactggcgg
    71281 gtgatgtggt aatacttagc acctttcttc gcggcacgtt tcgcaacctg ataggagact
    71341 tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt cgaacgggac catcatcgct
    71401 gctgtcgctt tgttcagttc ttcgacttta gtgccatccg ggagcgtgac ggtgtaacgc
    71461 ccgccttttg atgactgggt ttcaaagaag cgaccgactt cagaacttgg tgatgcggta
    71521 gtcgcaacac ccgggatctc aactttcttc gcggcttcgc caccggcagc cagagctgca
    71581 cgtcctgctt cggaatctgc cgggattaca tccgggctct ggatgatacg tttcttagca
    71641 tcttttttat agatgaatgc agtaatacgc tggttaccgc cctggttggc atcgatttga
    71701 cgaacaatgt agaaagagta ggcacctttc gcttttgccg ctttggtgat ggcatcattg
    71761 acttctggct ggctacgata gaagccctgg acggtgaccg tgtcaaacgg ttcaatcaga
    71821 acagcctgat ctttcggcag ttcgacaaca ccgttaatta cgcgattact tgtttcttct
    71881 gctttttcag catcggcttt atagaggtca gcgaccacac gccagttacc gctgttacca
    71941 aaatcagaag tgtcgacaac ataaaaagag gcggcacctt ctttatctgc gcgacgagaa
    72001 acggctttca ccgcttcgcc aatagcatta aaacgaccgg taaccactac acggtcaaaa
    72061 ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac ggagaatgcc
    72121 gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa gcttcataaa aataatcctt
    72181 cgccttgcgc aaaccaggta ctggtattgt tattaacgag aaacgtggct gattattgca
    72241 tttaaacggt gtaactgtct gcgtcatttt tcatatcaca ttccttaaac caattttaat
    72301 tctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat caccatcaat actactgaag
    72361 caactgaatt gtataagtta atttaatgtt aagtagtgat ttgtgccggg gcgatgtctc
    72421 gttttacccg gccgccgaag acaattatca gtctttatcc ggcgttctaa ggtgtttatc
    72481 ccactatcac ggctgaatcg ttaatatttt gcgagttcac gccgaaatac tgatttttgg
    72541 cgctagatca caggcataat tttcagtacg ttatagggcg tttgttacta atttatttta
    72601 acggagtaac atttagctcg tacatgagcg gcttgtgtgg ctcctgacac aggcaaacca
    72661 tcatcaataa aaccgatgga agggaatatc atgcgaattg gcataccaag agaacggtta
    72721 accaatgaaa cccgtgttgc agcaacacca aaaacagtgg aacagctgct gaaactgggt
    72781 tttaccgtcg cggtagagag cggcgcgggt caactggcaa gttttgacga taaagcgttt
    72841 gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga gatcattctg
    72901 aaggtcaatg cgccgttaga tgatgaaatt gcgttactga atcctgggac aacgttggtg
    72961 agttttatct ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga acgtaacgtg
    73021 accgtgatgg cgatggactc tgtgccgcgt atctcacgcg cacaatcgct ggacgcacta
    73081 agctcgatgg cgaatatcgc cggttatcgc gccattgttg aagctgcaca tgaatttggg
    73141 cgcttcttta ccggacaaat cactgcggcc gggaaagtgc caccggcaaa agtgatggtg
    73201 attggtgccg gtgtcgcagg tctggccgcc attggcgcag caaacagtct cggcgcgatt
    73261 gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag ttcaaagtat gggcgcagaa
    73321 ttcctcgagc tggattttaa agaggaagcg ggcagcggcg atggctatgc caaagtgatg
    73381 tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa agaggtcgat
    73441 atcattgtca ccaccgcgct tattccaggc aaaccagcgc cgaagctaat tacccgtgaa
    73501 atggttgact ccatgaaggc gggcagtgtg attgtcgacc tggcagccca aaacggcggc
    73561 aactgtgaat acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt caaagtaatt
    73621 ggttacaccg atcttccggg ccgtctgccg acgcaatcct cacagcttta cggcactaac
    73681 ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg gcaacatcac tgtggatttt
    73741 gatgatgtgg tgattcgtgg cgtgaccgtg atccgtgcgg gcgaaattac ctggccggca
    73801 ccgccgattc aggtatcagc tcagccgcag gcggcacaaa aagcggcacc ggaagtgaaa
    73861 actgaggaaa aatgtgcctg ctcaccgtgg cgtaaatacg cgttgatggc gttggcaatc
    73921 atccttttcg gctggatggc aagcgttgcg ccaaaagagt tccttggaca cttcactgtg
    73981 ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga atgtatcgca cgcgctgcat
    74041 acaccgttga tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg agcactgttg
    74101 cagattggcc agggcggctg ggttagcttc cttagtttta tcgcggtgct tatagccagc
    74161 attaatattt tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt ccgcaaaaat
    74221 taaggggtaa catatgtctg gaggattagt tacagctgca tacattgttg ccgcgatcct
    74281 gtttatcttc agtctggccg ggctttcgaa acatgaaacg tctcgccagg gtaacaattt
    74341 cggtatcgcc ggtatggcga ttgcgttaat cgcaaccatt tttggaccgg atacgggtaa
    74401 tgttggctgg atcttgctgg cgatggttat tggtggggca attggtatcc gtctggcgaa
    74461 gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc ctgcatagct tcgtgggtct
    74521 ggcggcagtg ctggttggtt ttaacagcta tctgcatcat gacgcgggaa tggcaccgat
    74581 tctggtcaat attcacctga cggaagtgtt cctcggtatc ttcatcgggg cggtaacgtt
    74641 tacggggtcg gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta aaccgttgat
    74701 gctgccaaac cgtcacaaaa tgaacctggc ggctctggtc gtttccttcc tgttgctgat
    74761 tgtatttgtt cgcacggaca gcgtcggcct gcaagtgctg gcattgctga taatgaccgc
    74821 cattgcgctg gtattcggct ggcatttagt cgcctccatc ggtggtgcag atatgccagt
    74881 ggtggtgtcg atgctgaact cgtactccgg ctgggcggct gcggctgcgg gctttatgct
    74941 tagcaacgac ctgctgattg tgaccggtgc gctggtcggt tcttcgggcg ctatcctttc
    75001 ttacattatg tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg gtggtttcgg
    75061 caccgacggc tcttctactg gtgatgatca ggaagtgggc gagcaccgcg aaatcaccgc
    75121 agaagagaca gcggaactgc tgaaaaactc ccattcagtg atcattactc cggggtacgg
    75181 catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt accgagaaac tgcgcgctcg
    75241 tggtatcaac gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg gacatatgaa
    75301 cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg acgagatcaa
    75361 cgatgacttt gctgataccg ataccgtact ggtgattggt gctaacgata cggttaaccc
    75421 ggcggcgcag gatgatccga agagtccgat tgctggtatg cctgtgctgg aagtgtggaa
    75481 agcgcagaac gtgattgtct ttaaacgttc gatgaacact ggctatgctg gtgtgcaaaa
    75541 cccgctgttc ttcaaggaaa acacccacat gctgtttggt gacgccaaag ccagcgtgga
    75601 tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg aggccgtcac tctttattga
    75661 gatcgcttaa cagaacggcg atgctttgac ctcccgctgt ttgttcaagc gcaattttga
    75721 caataattgt caacggcacg gaaagcagca tacccaccgg tcctaacaac catccccaaa
    75781 aaatcaacga caaaaatacc accaatgtgg aaagccccag cccacgtccc atgatgcgcg
    75841 gctccagaat attgccgaag accagattaa tcagcagata tcccgccagc accagcaacg
    75901 cttcgtagaa gccattaaac accagtacct gagcgatagg ggggattgcc gcgagaactg
    75961 aaccaatatt cgggatgtaa ttaagcgcaa aggccagcaa tccccagaca aaagcgaagc
    76021 gaacatcgag tgcggcgagc atcgcccagg cgaccaggcc ggtgatgatg ctgatggctg
    76081 ttttcagcac cagataatga gacacactgt caatcgcacg ctgaatcgcc gccatccctt
    76141 caaccggacg cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact tcgagcaaca
    76201 taaacagcac cgtcagcagc aataaaaata ttgatgacat ggcattagat aactgcgtca
    76261 ataagttggt gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc agctggtcaa
    76321 ctgagacatc aatccctacg cgttgcaaca acggttcaat cgcttgcagc ggcgtcataa
    76381 tagagttgcg atattgcggt aacgtccgcg tcaactcatt gagcgcggaa cccagataag
    76441 ctaacagcaa caccatcgcc atcacgatga tggtcatcaa aattgacact gccagtacac
    76501 gcggcacacg ccagcggacc atgtgttgca ccagcgggtt aagaataaca gcaataaata
    76561 atgcgagaat aaacggcacg atgatctcgg cggcaaaacg gataccgcag agaataatga
    76621 ccagcattcc caacataatg acgattttta ggccattgag cgtgatgatc ggctttgcca
    76681 tgcttgtccc cgttttttct tcctttgcta ataataatgt ctttttcgct ccgtcatcat
    76741 aacgaaaatc aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat tcttaagcta
    76801 tgatacaaat caggcgtgtt caactaccga ggacaattat catccgcgag gacgagaagc
    76861 aacactgcgg ataattgtaa tattatggac aatttgttca ggacgttatt ttctacattt
    76921 acccatcttc gtacctcatc cactatcttg ctcgtaggtg agcagcactg gcgcaacgcg
    76981 ctttagtcct ttcttctgcc aaagcttgcg ctaagcgcgg ggcttgacca ctttactttg
    77041 gtttcgctga attaagcgaa aattaaaata attctcttgc aggagaagga caatgtatat
    77101 ttattggatt ttattaggtc tggctattgc tacagaaatt accggtacgc tgtccatgaa
    77161 atgggcgagc gtcagtgagg gaaatggcgg ctttatttta atgctggtga tgatttctct
    77221 gtcgtatata tttctctctt tcgccgttaa aaaaatcgcc ttaggcgtgg cttatgcgct
    77281 gtgggaaggt attggtattt tatttattac cttgtttagc gttttgttat tcgacgaaag
    77341 tttatcgctg acgaaaattg ccgggttaac caccctggtg gccgggattg tgttgatcaa
    77401 gtcaggtacc cgtaaagcgc gtaaacctga actggaggtg aaccatggcg cagtttgaat
    77461 gggttcacgc cgcctggctg gcattggcaa tcgtgctgga aatcgttgct aacgtctttt
    77521 tgaaattttc tgacggcttt cgtcgcaaaa tatttggctt gctctctctg gcggcggtgc
    77581 tggctgcctt tagtgcgctt tctcaggccg ttaaagggat cgacttgtct gtcgcttatg
    77641 cattgtgggg cgggtttggt attgccgcca cgttagccgc aggttggatc ttgtttggtc
    77701 aacggttaaa tcgtaaaggc tggattggcc tggtgctgct gttggctgga atgatcatgg
    77761 tgaaacttgc ctgatgaaca cgctgcccgc gctgtcgggc agcgtttgaa cattattttt
    77821 gcgacagttg atccagcttg tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc
    77881 gccagcgatc tttcgcggca ggagccgagc tttgcacccc aattaattgc cagccgtcat
    77941 cggtatgcaa catcagaggc gaaccgctgt cacccggcaa ggtatcgcac tgatgcgaca
    78001 tcaccgacgt ttgcgcccag ccagccactt cacagttttg atgactgtac aacgtatcga
    78061 gatgatcttc agggtagccc gcctgagtca ctttacgacc agccgctttt aatgcggcgg
    78121 taagcgcggc tttatctccc tcaaataacg gcaacggtgt aatgccagaa gggggattac
    78181 gtagcacaat caaaccgaag tcccagggcg cagctgcggg aggtacaatc cagccatccc
    78241 catctgcttt taaccgcttt cccagtgtcg gatcaacgcg gccttctatg tcgtggatct
    78301 cataacgcca aagaccttta tttgacacga aacgcagcgc taccgcttta tcggctttac
    78361 cctttggcgg cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt gcaatcagcg
    78421 tcgccgtaca taaattgccg ctggccgttt ccagttggcc aaccgcatcc cacggtgatt
    78481 gggtcgtgtc attcactggc acacgatcat catgaccaaa aaacagggtg ctgacctcat
    78541 cgtttgccga tttggcaacg tctggtttat ctgcaaacac aaaagcagac gtcaaactaa
    78601 ttgcacccaa cactacagca atggttgtac gcatatcaca ctctggtggg taattatgat
    78661 tattaaaagc aaaccctcat aaatactata gacgggacgg gatgaaagtg ggagtaaaat
    78721 cagataacta cgatcaggaa agataaaaac gggcggcaat cagtgcgcat aaaataagga
    78781 tgataagaat gaactcaaag cgataacggc ccaccatact accctccgct aaaggcggtg
    78841 ctaaacgtta cccgcttagc accgtaattt tcaggcgcga gggggcgcgc cagcattact
    78901 gttgaaaact tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat ggctgtgttt
    78961 cttggcgtgt ttcttcgccg cctgcgcttt ttgttcaggg gctttctgtt cagctttggc
    79021 atttttatga tgctttttag ccgcctgcgc tttctgggca ggtgctgctt tatgctgttt
    79081 tttatgatgt gtggttttcg caggcgctgc tttggtggtc gtcgcagtcg gagcaggtgt
    79141 ggtcgcagtc tctgcagcaa aggcagcaga agacagaccc atagcagcgg caacaaccag
    79201 agctaatact tttttcattg tcataccctc aatttgtttt ttcatttaac cccactgcgg
    79261 ggccgttgaa ataactatat ccctgggaat ggcagacttc cgtgagtggt tggtttcagc
    79321 gtgtaacgat atgtacaaac gctgaataga ttacagcatt gataataacg tattgtgact
    79381 taaggaaaat ttagctacca ataatagtag tcttgatcgt gttaactatt ttactactta
    79441 caaagcggta gaataactgc gcatcagtaa taaatgacac acagcaaaat gaatccgttt
    79501 atttgggtac ttataatcct gatgacgcta gatgcgctgc gggaattggc tggcgcttcg
    79561 tctattttag gatggctatt aacgttggtt tgagcaggca ataagtccgg acggggattt
    79621 acctcagtcc ggacttattt tttcaggcgt gcagacgatg atgcaaacgc gtcccgacca
    79681 gcagagcaat gaccagcatc agagcaataa atgcgccgac gccgttccag ccatagttat
    79741 gccagaaaac accacccagc gtcccggcaa tactcgaccc cagatagtaa ctgaacagat
    79801 acagcgagga ggcctggcct ttagcgcgtt ttgcgcgggg gccgatccag ctgctggcaa
    79861 cggagtgggc tgcgaagaat ccggctgaga agagtaacat tccggcaaag atcagccaca
    79921 gcgagctgaa taaggtcatc agtaaaccaa acagcataac ccccgtcgaa aacaacatca
    79981 ccggaccgcg tccatagcgg gtggtcatgg ttccggcttt gggtgagctc catgtaccgg
    80041 tcaaataagc cagcgataat aagccaacca cggcctgact gacatgccag ggggaaagca
    80101 tcaacctata gccgatgtaa ttaaacagcg tgacgaacga ccccatcagc aaaaagcctt
    80161 ctgcgaacaa taacggtaat ccccggtcac gccagtgcag acgaaagttg ataaataacg
    80221 tcttagggcg cagagaagtc gggcgaaaat ggcgtgattc agggaggatt ttccagaaca
    80281 tcaacgccga ggccagcgcg aaacaaccga ttgccgccag agcaattcgc cagttgaaaa
    80341 agtccgtgaa gacaccgcta attaagcgtc cgctcatgcc gccaattgag ttgccgctga
    80401 tatacaaccc cattgaaaag gccacgaaac tggggtggat ttcctcgcta agataagtca
    80461 tgccaacggc tgccacccca cttaacgaaa gcccaatcaa ggcgcgcata atcaaaatgc
    80521 cgtgccagct ggtcatcatt gtcgaaagta acgtacaaat ggaggccaac agtagcgccg
    80581 tgaccatcac tggtttgcga ccaatggcat cggatagcgg gccagtaaac agcaaaccga
    80641 tagccaacat cgccgttgaa atggacagtg aaatactact gttcgcgggt gttaagccaa
    80701 actcctgcga aagcaccgga aggataggtt gcacacaata gagaagtgcg aaggttgcca
    80761 gtccggcaga gaaaagcgcc agggtgacgc gcataaattg cggagtaccg cgtttaataa
    80821 attgatttgg ctgagaaatg ctttgcttgt cagtgtcgct tgccggagcg ccatcaacag
    80881 ttgtagtacg gctcacttga aatccttgct aaatatgcct gtggatcagg cttatgaata
    80941 gattaggaaa atcgaattgt tctgtctaat atattaataa tctcaaataa gatgttttaa
    81001 atatgaatat tgaacttcgt catctgcgtt actttgtcgc tgttgcagaa gagctgcatt
    81061 tcgggcgtgc cgctgcccgc ctgaatattt cgcaaccacc tctaagtcag cagattcagg
    81121 cgctggagca acaaattggt gcccgactgc tggcacggac caatcgcagt gtattgctga
    81181 cggaagcagg aaaacagttt cttgtagata gtcggcaaat cctgtctatg gtggatgatg
    81241 ctgccgctcg cgctgaaagg ctgcatcagg gcgaagcggg ggagttgcgc attggtttta
    81301 cttcgtccgc tccttttatt cgggcggtgt ccgatacgtt atcgctgttt cgccgtgatt
    81361 atcccgatgt ccatttacaa acccgcgaaa tgaacactcg cgagcaaatc gctccgctca
    81421 ttgaaggaac gctggatatg ggattgctgc gtaacacagc gttaccggag acgcttgaac
    81481 acgcagtcat cgtccatgaa ccgcttatgg cgatgatccc gcacgatcat cccctggcaa
    81541 ataacccgag tgtaacgctg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc
    81601 acgtcggaac agggctgtat gacgatattc tcgggctgat gagacgttac aatttgacgc
    81661 ccgtcatcac tcaggaggtg ggtgaggcaa tgaccatcat cggtctggtt tccgccggtc
    81721 tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg
    81781 tgacgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg
    81841 aacaaagtcc agctgcgcga aactttcgta ttcatctgct gaatgctctc aggtgaggga
    81901 aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga
    81961 cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag
    82021 ggagtatgcg gtggttgctg aaaatcagcc tgggcacatt gatcaaataa agcagaccaa
    82081 cgcaggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc
    82141 ccgtctggcg caactggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc
    82201 acacctggtg caagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg
    82261 gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat
    82321 tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc
    82381 gttaaaagat gactcgccat tgctggatcg tatcatttcc catatcgatc agttttttat
    82441 ccgccaccag aaaaaacttg agcgtctgac ttcgattgcc ataaccttgc cgggaattat
    82501 tgatacggaa aatggcattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc
    82561 gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatatccagc atgatatcag
    82621 cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca
    82681 ggtggttatc gatcacaacg tgggggcggg cgtcattacc gatggtcatc tgctacacgc
    82741 cggcagcagt agcctcgtgg aaataggcca cacacaggtc gacccgtatg ggaaacgctg
    82801 ttattgcggg aatcacggtt gcctcgaaac catcgccagt gtggacagta ttcttgagct
    82861 ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catggacaac cgttaaccgt
    82921 ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattaccgg
    82981 ggtaggcgcg catgtcggtc gcattcttgc catcatggtg aatttattta acccacaaaa
    83041 aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccag tcatctcgga
    83101 cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca
    83161 gttttctaac cagggcacga tggcaggcgc tgcgctggta aaagacgcga tgtataacgg
    83221 ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc
    83281 gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat tattttcctg
    83341 gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg
    83401 gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcct
    83461 cccagggaaa aacggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg
    83521 aagggctgcg taataaagat gccctggtgt tgcagagtgt ttcaaccatc gaactgcctt
    83581 atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc
    83641 caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa gtcgatcatg
    83701 tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccgctctctg
    83761 aatgggtggt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca
    83821 ttaaccatgc actgctaaca gctcaggcga ttgccaacga cgggctgccg ctcattggct
    83881 gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctcg
    83941 gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc
    84001 gtgaactggg gcaatacatc cgcttagcta tgcttcgcag tgtgctggcg gtagatagag
    84061 tcacggtgta acgtccgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg
    84121 agtagctgat actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc
    84181 gtggtcgccg ccaacagtgt cgccatccct gtcaatccga gtaaaagcgt aatttcttcg
    84241 ccatcaggta accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata
    84301 aatagcgtcg gtgtaaagac accaccgggc gcgccagaac cactactcgc cagcacggca
    84361 cacagtttac agaggaagat cccggcaatg atcattaaca gtggcggagc ggttaaaaag
    84421 gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc
    84481 acgatcaacc cgcccagtgc cagttgccag ggcggcgcaa gcttgagact cacaaatcca
    84541 cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct
    84601 gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgctgtag
    84661 agtaatgcgt cgctatgatt aataagattg ctaatcagca acgccacgac ggcggaaata
    84721 atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa
    84781 ctgccagcaa gcggggcgcg ataggccgca gccattcccg ccgcagctcc acaggcgatc
    84841 cataatttcc actcctggcg tggcgtaaaa cgttgggcaa aacacgaggc tgcaagggca
    84901 gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagtaga
    84961 gaggcaagcg atttaaccag gcttgctgcg taatcaaact gaccatcggt ttgcaacgct
    85021 tccatgtaat cggtcggcgc atgagggcgt tgctgggtaa atttctgcca gcccatcagc
    85081 aacaaacccg ccgccagtcc gccgagcgcc ggggttagca gccgtcgcca cggggaaaga
    85141 tttgtcgctg cattgaccag actgccggag ttattattga ggaacagcca ctccagtagc
    85201 agcatcgcat gacgaaaccc ggcaacggca aaggccgcaa gaataccgac gattgttgcg
    85261 ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc
    85321 agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccag ccgcaaaagc
    85381 agtgcgtgct ttaagagata aaaaacgtga cactgtaacc tattaattgc cgaattgcgt
    85441 gtaatcgtca ccatccggca ataatacggt gatcctatcg aaacagcggt ttaaccgcga
    85501 caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagcgagt tcacccagtg
    85561 cacggtagaa agggtgttct gctttttcga taaaaacgtc aagaaaacgt gttgaccacg
    85621 gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc
    85681 acgcagccat cagcagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcga
    85741 actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca
    85801 cagattcgcg atccagccag accgatcccc acggcggtga cggcagtgcc cacgggccga
    85861 caaacaaacg ctgccaggcc tgggcgtgag tctcttcact ctgtgtctga aaagcagtca
    85921 ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagctg tcattggtga
    85981 gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac agcgcgccca
    86041 acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc
    86101 tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt cctaccgtca
    86161 tgtgcaggcc ataaaagagc acacgcccga tcatttcacc gccaagaatg aggattaacc
    86221 ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa agccagcatc
    86281 ctagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca
    86341 gtgcgctggc ttgttgtacg gatgagtgaa tagacgccag agacaaacct tgcagcacaa
    86401 tcaccccggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt gtggtattaa
    86461 atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc aacaatactg
    86521 tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcactgtg tcgatttgat
    86581 acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga
    86641 gtttccccaa cgcttgcggc attttaccga tgactgccac cagccaccat aatccgccaa
    86701 cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaatct ccgattcgat
    86761 tcagcgagtt aaacgcccgc agtggcgacc ccaggtgcat gacagaggca ataaacccga
    86821 tgcccatcaa cagccagagg aaaaacatgc cacggacaat acgctgacgg tctgcgtcat
    86881 ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca
    86941 atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc
    87001 ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttgccgtt aggtttgata
    87061 acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg caacggcagc cagagtgccg
    87121 tgcttctggc gcagctcttc aattggaccg aattcaagtg cacgcagcgg gcaggattcg
    87181 acacatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca cttcgtcatg
    87241 tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg gcagtagcgg
    87301 cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg
    87361 ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga
    87421 taataagcga acacgttctg gtgccagacg ccattatctt cctgccagtc gccgccagca
    87481 tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc
    87541 agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg
    87601 gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc
    87661 ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc cgtgatcgac
    87721 ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg
    87781 cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattagtgt taaatacccg
    87841 cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atagggttga tccacacctc
    87901 ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt
    87961 gtagtggaag cccgttaact gcagtggata ggttttccgc aggggatcgt cccagccatc
    88021 aaaacctggc gtataagctg gaaggggatg aataatttca tcttttttca attcccaggt
    88081 atccgcaatc gtcgccagtc gttcagaata aatttcgatt ttccccgaag gtgttttcaa
    88141 cgggtttgtc tgtggatctt cacggaatgc gcggaaagcg acgtagtgtt cttccgggca
    88201 ttttttctta aagatcccgg tcgttttcat ctcctcgtag tcgggcatct cagggttacg
    88261 ttccttcgtt ttcgcatgga gatatttgat ccattcatgc tgactgcgac cttcagtaaa
    88321 ggtttgataa acgtctggtc ctaagctttt ggcgacttca ctcagaatcc agtagatggg
    88381 tttgcgttca aattttgctg aggttgcggg ttgggcgagg atcacatagc ccatattccc
    88441 tgcagattca tgagagataa ggtcttcttg ctctgttggc atcaggtcgg gcaacaggat
    88501 atcgcaatac ttagccgagg cagtcatgaa gtggtcaatg ccaacaatca tctcgcactt
    88561 gctgtcatcc tgaagcacct catgggtgtg attgatgtcg ccatgttgat tgatcaatgt
    88621 gttactggcg tagcaccata aaaacttgat ggggacatcc agtttttctt ttccacgaac
    88681 accatcacgg gtcgcggtca tttccgtacc gtggtcgatg gcatctgtcc atgtaaagac
    88741 ggaaatctgc gttttaacag gattctcgag catcgggaac cattctactc ccagatccca
    88801 gctaccttcg cgtacacctg agttgccgcc gtttatgccg acgttaccgg tgagaacgga
    88861 aagcatggca atagcgcggg atgtttgctc gccgttggaa tgtcgttgtg gcccccaacc
    88921 ctgacaaata taagccggtt ttgctgaacc gatctctcgt gccaactgga taattttttc
    88981 tgccgggatg ctggtgattt ttgctgccca ttccggcgtt ttagctatgc cgtcaggccc
    89041 ttcgcccaga atataggctt tataatgcgc gttacgtggt gcgttggcgg gcagcgtttt
    89101 ttcatcgtaa ccaacacaat atttgtcgag aaatggctga tcgaccatgt tttcagtaat
    89161 cagtacccag gcaatcgcac aggccagtgc gccatcggtg ccagggcgaa tgggcagcca
    89221 ttcatcttca cgcccggcag cagtgtcgtt ataacgtgga tcgatgacga tcatgcgtgc
    89281 atttgaacgt tcgcgggctt gctcgacgta gtaagtgaca ccaccgccgc tcatccgggt
    89341 ttctgccggg ttatttccga acataacgac cagtttcgta ttggcgatat catccgggct
    89401 gttgccatca ttggcaccga acatataact catcgcggca ctgatctgtg cggtactgta
    89461 gctgccatag cgactgagaa aaccaccgca agagttcatc agacggtacg ggacgttcga
    89521 gttggtgatg tttccgccat ctacgcctgt tccgtacagg acatgtacag cctcattgcc
    89581 gtaatctttc aggatccgcc gaagattatc actgatggta tccagggctt catcccaact
    89641 tatccgttca aatttacctt caccgcgctt gccgacgcgc ttcatgggat atttcaacct
    89701 gtcaggatga ttcatccgtc ggcggataga gcgcccgcgt aaacatgccc gaacctgatg
    89761 attaccgtaa acgtcgtctc ctgtcgtatc agactccacc cagtacacgg tgtcatcttt
    89821 cacatgcaaa cgtaacagac agcggctccc gcagttaacg gtgcaggaac tccagaccgc
    89881 tttctcttct accggtgtct ctgccgctcg gaccatttgg gaaaatggca gagtgaaagc
    89941 actgcttgcc agcgcaagac tgccaagtgc ggaggttttc atcagacttc tacggctgat
    90001 ttcagccttc atgagcgcct ctgtggtatg gattttcatc attactcact tattgctttt
    90061 caaacaaaat gtcatgccag aatttatggt tgtagagggt tatatttttt cgatctcgac
    90121 cagattagtg tgctgcgggt ttcccttcgc cagtggtgaa gggcgcagag tggttagcgt
    90181 attcacacag ccgccatggt cgattttatc gccagacata ttggcctcgt gccaggctcc
    90241 ctggcccata gcactaactc cagggagaat gcgtggtgtc actttggctg gtagccgaac
    90301 ttcgccacga tggttataca cccgcaccat atcgccgttg gcaatcccac gtttatgtgc
    90361 gtctataggg ttgatccaca cctcctgacg gcaggcagcc ttcaggacat caatgttgcc
    90421 gtaggtcgag tgagtacggg atttgtaatg gaaaccaaac agttgcagtg gaaaggtact
    90481 acgttcaggg gagtcccagc cttcaaaggt tgaggcataa accggcagtg ggcttatcac
    90541 ttcatctttt tccagttccc aggtacgggc aatttccgcc agcttgctgg aataaatttc
    90601 aatcttaccg gaaggcgttt taagtggatt tgcctcgggg tcgtcacgaa atgctttgta
    90661 ggcgacaaaa tgaccattgg gatctttacg cttatagata cccatttttt tcagttcgtc
    90721 gtaagacggt aacgccggat ctttggcaag cattttggcg tacagatgtt gtaaccattg
    90781 ttcctgagtg cgaccttcag tgaacttttg atagacgtca ggtccaagac gtttcgcgac
    90841 ttcactcagg atccagtaaa tcggtttgcg ttcgaatttt tcgctggtga ctggctgaag
    90901 gaaaatgaga tatcccatgt taccggcgta atcgttagga ataatatctt cctgctcaac
    90961 ggtcatcagg tctggcagca gaatgtcggc atattttgcc gatgaggtca taaagttttc
    91021 gatgaccaca atcatttcgc atttcgattc gtcctgcaga atttcatgcg ttttgttgat
    91081 gtcagaatgc tgattaacca gggtatttcc cgcgtagttc cagatgaact taatgggcac
    91141 atccagttta tctttgcccc ggacgccgtc gcggattgcc gtcatttgcg ggccatgatc
    91201 gatagcatcc gtccagctga agcaggagat tgacgttttg accggattat ccagcaccgg
    91261 caggcgttct atggtaatgg tataggtcga ttcacgcgcg ccactatttc cgccgctgat
    91321 gccgacattg cccgtcaaaa taggcaacat agcaatagcg cgtgccgtca gctcaccgtt
    91381 tgcctggcgt tgcggccccc agccctggca gatataagcg ggttttgctg tgccaatttc
    91441 acgcgccagc ttgatgatac ggtccaccgg gataccggta atttgcgaag cccactgcgg
    91501 cgttttcgct gttttatcat caccttcacc aagaatatag gctttatagt gaccattttt
    91561 tggtgcatct gcgggtaagg ttttttcgtc atagccgacg cagtatttat cgagaaaagg
    91621 ttgatcaacg agattttcgt taatcaatac ccaggcaata cccgcaacca gcgcggcatc
    91681 ggtgcccggg cgaataggaa gccattcatc ttcacgacca gcagccgtat cggtatatcg
    91741 cggatcgata acaatcattt ttgcgttcga tttctcgcgt gctttttcaa gaagataagt
    91801 gatgccaccg ccgctcatgc gggtttcagc cgggttgtta ccaaacatca cgaccagctt
    91861 gctgttttca atatccgtgg tgctgttgcc atcattactg ccgtaggtgt agggcatggc
    91921 acaggaaatt tgcgcagtgc tgtaggagcc atactgattt agtgaaccgc cgtagcagtt
    91981 catcaggcgt ttgaccgccg aggctgatgg agaagagcgg gtcatattgc cgccaacaat
    92041 ccccgaagag tactgaatat atacagcctc attgccatat tgtttgacgg tttttttcag
    92101 gctactggcg atagtatcca gggcttcatc ccagctaatc cgttcgaatt tgccttcgcc
    92161 gcgtttgccc acgcgtttca ttgggtaatt caggcgatcg ggatgattaa tacgccgacg
    92221 gatggagcga ccgcgcaaac aggcgcgtac ctgatggttg ccatactcgt cgctgccggt
    92281 gttgtcagtt tccacccagg tcacttcatt atctttaaca tgcagacgaa gtgcacagcg
    92341 gctaccacag ttgacggaac aggcacccca gaccactttt tcgttggcct gttgtaccgc
    92401 tgcttctgca ctgcgcaggg taaacggcaa agaaaaaccg cctgcagcca gcgccagaga
    92461 acctatcgcg gtagatttaa cgagtgttct gcggctgatg cccaccattt gttcattttt
    92521 ggacataact cactccctgt tctttatcgt tatataaatg tttatatatt gaatatttag
    92581 cgcgctaaca atagagagag tctacccatt tggggttaat aattattaat ccatatcaat
    92641 agaagggtat gagtaataag gtgggattat gttgtatgtt caaatcgccg gatgtgtcgt
    92701 atccggcgtt cagtcgataa tgtgttactg cggttcagca ggcgcgttat cctgggtaga
    92761 ctgcgcggga gcagagacgt taccgctggt ggtacgggtg tagagaattt tatgcgtatc
    92821 attagcgcaa tgcccgacga cctgggaatc aggctgatca acctggtcat tgggtacaat
    92881 acttaacgtg aagctgcttt cgggtacgcc attattgata atgcgctgtg atatatcgct
    92941 ctgtatgcgc tcacaggatc ccggcgcggc gagtaccgcg ggtgaggcga gggcgagcag
    93001 aagcgcggca cagcaggttg agagtttcat cataagctcc ttacgcgaag ataacttctt
    93061 taagcatagc atttaacgtg taaagtactg tatttgctac tatgattgag aatcatctct
    93121 actctcaggt gactgttgtg aaatacaaat tactgccatg cttactcgcg atattactca
    93181 caggatgtga ccgcacagag gtaacacttt catttacccc ggagatggcc agtttctcta
    93241 atgaattcga ttttgatccg ctgcgtggtc ccgtaaaaga tttcactcag acattaatgg
    93301 atgagcaagg tgaagtgacg aaacgtgttt cggggacttt gtcggaagaa ggctgttttg
    93361 attctctcga attactggat ctggaaaata ataccgtggt tgccctggta ctggacgcca
    93421 attattaccg tgatgccgag acgctggaga aaagagttcg tttgcaggga aaatgccagc
    93481 tagcagaatt accttctgcc ggggtgagtt gggaaaccga tgataatggt ttcgtgatta
    93541 aagccagcag caaacagatg cagatggaat atcgctatga tgatcagggg tatccgctgg
    93601 gtaaaaccac gaaaagtaac gataaaacat tatctgtcag cgccacgcca tcaacggacc
    93661 cgatcaaaaa attagattac acagcggtta ctttactgaa taatcaacgg gttggaaatg
    93721 taaaacagag ctgtgaatat gacaatcacg ccaatccggt ggactgtcag ctaatcattg
    93781 ttgatgaagg agtaaaaccc gccgtcgaac gggtttacac catcaaaaat acgatcgatt
    93841 attattaata ctattgtgcg gtcggcttca ggagagtctg acccggtgtt ttgtgctcag
    93901 ccagatactg atgctggaat atacacatgc gaatggcatt acgatattga tcattaataa
    93961 agaactcatg catcaattca ccttcaaccg taaagccaag cttgcggtaa atgtgaatcg
    94021 ctttttcatt ctctttatca acgatcagat acagcttata gagattgaga acggtgaagc
    94081 catagtccat tgctaatttg gcggcacggg ttgccagacc tttcccctga tactccgggg
    94141 agataattat ctgaaattct gcgcggcgat gaacatggtt aatttccacc agctccacca
    94201 gaccggcttt ttcgccgtca cattccacca caaagcgccg ttcgctctga tcgtgaatat
    94261 gcttatcata cagatcagag agttcaacaa aggcttcgta gggttcctca aaccagtaac
    94321 gcatcacact ggcgttattg tcgagttgat gtacatagcg taaatcttca cgctccagcg
    94381 ggcgtagctt aacactgtgg gcgcttggca taacgtgtcc ttacattcct taaatcaata
    94441 acaggttagg ggataataac gcggccagtt cgacggtcca ggcagcgcaa agtatttggc
    94501 tcccagtagg cattgatgtt ggcgctttgc tcacatttat cgcggttatc aaaagcagcg
    94561 tcggctttat cccactcttt ttcagtgcgt ttattcactt tctggcgcag attacgcgtg
    94621 tcattccatt gctctttttc catagcggcg cgctggcggc tttgtgcact gtcgccagac
    94681 tcaatcacca gtttgttagt ttcggcatga acagttgtgc tcaatgccag tgcgcaaggc
    94741 agcagaaaag cgagcaggcc gattcgtttg ctgagagtga ttttcataat tcattccctg
    94801 tatgaatgat taaagctgat tctacaccat ccactgcgaa cgcaaaacgt tcaaggaggg
    94861 tgtttatatt gatgatatta tgtcgcccta taactatacg tgatgtcaat aagtgacaaa
    94921 gatgattaaa acaacgttac tattttttgc tactgcgctg tgtgaaatta ttggatgctt
    94981 tttgccctgg ctgtggttga agcgaaacgc cagtatctgg ctgttgcttc cggcggggat
    95041 ttcactggcg ctgtttgtct ggttgttaac gttgcatcct gcggcgagtg ggcgtgttta
    95101 cgcggcttat ggtggcgttt atgtctgcac ggctttgatg tggctgcgcg ttgtggatgg
    95161 cgtgaaactg actctttatg actggacggg ggcgttgatt gcgctttgcg gcatgttgat
    95221 cattgttgcg ggctgggggc gcacgtagga acataaatcc attttatcaa taagatacga
    95281 ggaagtgtca gctgacaaaa ggtattctgt ttcatctttt gtcaaccatt cacggcgcaa
    95341 atatacgcct ttttttgtga tcactccggc tttttttgat cttcatactt gtatggtagt
    95401 agctcagttg cgtagatttc atgcatcacg acaagcgatg caaggaatcg aacatgaaga
    95461 tcgtaaaggc tgaagttttt gttacctgtc cggggcgtaa tttcgtcaca ttaaaaatca
    95521 ccactgagga cggtattacg ggccttgggg atgccaccct aaatggacgt gagctttccg
    95581 tggcctctta tttgcaggat cacctttgtc cgcagcttat tggtcgcgat gcacaccgta
    95641 tcgaagatat ctggcagttt ttctataaag gtgcttactg gcgtcgcggt ccggttacga
    95701 tgtcggccat ttcagcggtt gatatggcgc tgtgggatat taaagccaaa gctgccaaca
    95761 tgccgcttta ccagttactc ggcggcgcgt ctcgtgaagg ggtgatggtt tattgccata
    95821 ccaccggtca cagtattgat gaagctctgg atgattatgc ccgtcatcag gagctgggat
    95881 tcaaagccat ccgcgtgcag tgcggaatcc ctggtatgaa aaccacctac ggcatgtcga
    95941 aaggtaaagg tctggcttat gaacccgcaa ccaaaggaca gtggccggaa gagcagctgt
    96001 ggtcgacgga gaaatacctc gatttcatgc cgaaattgtt tgacgcggta cgtaacaagt
    96061 ttggttttga tgaacatctg ctgcatgaca tgcaccatcg cttaacgcct attgaagcgg
    96121 cgcgctttgg taagagcatt gaagattatc gcatgttctg gatggaagac ccgacgcctg
    96181 cggaaaacca ggaatgcttc cgtctcattc gccaacatac cgtcacaccc atcgcggtgg
    96241 gtgaagtctt caacagcatt tgggactgca aacaactgat tgaagagcaa ctcatcgatt
    96301 atatccgcac cacgctgacc catgcaggcg gaattaccgg tatgcgtcgg attgccgatt
    96361 ttgcttcgct gtatcaggta cgtactggct cacacggtcc ttccgatttg tcaccagtct
    96421 gcatggctgc ggcgctgcac tttgatctgt gggtccccaa tttcggtgtc caggaataca
    96481 tgggttattc cgaacaaatg ctcgaagtct tcccgcacaa ctggactttc gataacggct
    96541 atatgcatcc gggagaaaaa ccgggtcttg gcatcgaatt cgatgaaaag ctggcggcga
    96601 aatatcccta tgaacctgct tatctgccag tcgcacgtct ggaagatggc acgctgtgga
    96661 actggtaagg agtaaggtaa tgaaaagcat tttaattgaa aaaccgaatc aactgtcgat
    96721 tatcgaacgt gaaataccca ccccgtcagc gggtgaagta cgagtaaaag ttaaacttgc
    96781 cggaatttgt ggttcagata gccatattta ccgtgggcat aatccttttg cgaaatatcc
    96841 gcgcgtcatt ggtcatgaat tctttggcgt cattgatgca gtgggtgacg gcgtggaaag
    96901 cgccagagtc ggtgaacgcg ttgctgtcga tccggtggtc agctgtaggc attgctatcc
    96961 gtgctctata ggtaagccga acgtttgtac gacacttgct gtattaggtg tgcacgctga
    97021 cggtggtttc agtgaatatg ccgtggttcc ggcaaaaaat gcgtggaaaa ttcctgaagc
    97081 agtggccgat caatatgcgg tgatgatcga accttttacc attgcggcta acgttaccgg
    97141 tcatggtcaa ccgactgaaa atgataccgt tctggtttac ggtgccggcc caatcggcct
    97201 gacgatcgtt caggtattaa aaggcgtcta taacgttaaa aatgtgattg ttgccgatcg
    97261 cattgatgaa cgactggaaa aagcgaaaga gagcggggca gactgggcga ttaataacag
    97321 ccagacaccg cttggcgaga gtttcgctga aaaaggtatc aagccgactt taattatcga
    97381 tgccgcttgt catccttcaa tcttgaaaga ggccgtaacg ctggcttctc cagcggcacg
    97441 tattgtattg atgggcttct ccagtgaacc gtctgaagtg attcagcaag gaattaccgg
    97501 aaaagaactc tctattttct cttcacgctt aaatgcaaat aaattcccgg tcgttatcga
    97561 ctggttaagt aaagggttaa ttaaaccaga aaaactaatt acccatacat ttgatttcca
    97621 gcatgttgcg gatgccatta gtttatttga acaggatcaa aaacattgct gcaaagtctt
    97681 actcactttt tctgaataat accaataacg gcgagtaagt agtacgcatc ttacctcttt
    97741 tttagagata accattatga caatagaaaa acacgaaaga agtactaagg atttggtgaa
    97801 agcagcagta tcgggatggt tgggcactgc gcttgaattt atggacttcc agttatattc
    97861 gctcggcgca gcgttagtgt ttcatgaaat attttttcct gaatcatcaa cggcaatggc
    97921 gttaattctg gcaatgggaa cctatggtgc aggttatgtg gcacgtattg tcggagcatt
    97981 tattttcggc aaaatgggcg acagaatagg gcgtaaaaaa gtgctcttta ttaccatcac
    98041 catgatgggg atctgtacca ccttaattgg tgtgttaccg acctatgcac agattggtgt
    98101 ttttgcaccc atcttgctgg tgacgcttcg tattattcag gggttgggtg caggtgcgga
    98161 aatttccggt gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat
    98221 ctcctcattt gtggctatgg gaactaactg cggaaccttg agcgcaacag caatctgggc
    98281 ctttatgttc ttcattctca gtaaagagga actgctggcg tggggatggc gtataccttt
    98341 cctggcgagc gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag
    98401 cccggtcttt gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg
    98461 tagcatgttc cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggctgg
    98521 taactcaggt ttaattcaga ctttccttgc aggctattta gtgcagacgt tattgtttaa
    98581 caaagcaatt ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc
    98641 gttccttggt tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac
    98701 ctctgcgatt gtgctggcat ggccaatgct ttctatcatc gtagataaaa gctatgcccc
    98761 gagcaccatt atggttgcac tgattgtgat tcataactgt gcggtgcttg gattatttgc
    98821 tctggaaaac attaccatgg cagaaatgtt cggctgtaaa aaccgcttta cccgaatggc
    98881 tatttctaaa gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcggggat
    98941 tttctgcacc atgacggaat cctggtatcc gatcgccatt atgatcatgg catattctgt
    99001 gattggttta atctctgcgc tgaaaatgcc agaagtgaaa gaccgtgatt taagtgcgct
    99061 ggaagacgcc gcagaagatc aaccacatgt tgtaagagct gcgcaaccgt ccagaagtct
    99121 gtaaatcctc aatcccttct cttaccggag aggggatttt tattcat
//
LOCUS       DAZFRE010000003        92648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFRE010000003 DAZFRE010000000
VERSION     DAZFRE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 92648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.003"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            97..1230
                     /gene="ompN"
                     /locus_tag="ACHKUI_000234"
     CDS             97..1230
                     /gene="ompN"
                     /locus_tag="ACHKUI_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO6596515.1"
                     /translation="MKSKVLALLIPALLATGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNADFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1371..1805
                     /gene="uspF"
                     /locus_tag="ACHKUI_000235"
     CDS             1371..1805
                     /gene="uspF"
                     /locus_tag="ACHKUI_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6596516.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1894..2495
                     /locus_tag="ACHKUI_000236"
                     /pseudo
     CDS             1894..2495
                     /locus_tag="ACHKUI_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(3722..3808)
                     /locus_tag="ACHKUI_000237"
                     /pseudo
     CDS             complement(3722..3808)
                     /locus_tag="ACHKUI_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            3850..4080
                     /locus_tag="ACHKUI_000238"
                     /pseudo
     CDS             3850..4080
                     /locus_tag="ACHKUI_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4132..5067
                     /gene="ttcA"
                     /locus_tag="ACHKUI_000239"
     CDS             4132..5067
                     /gene="ttcA"
                     /locus_tag="ACHKUI_000239"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6596517.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(5196..6569)
                     /gene="dbpA"
                     /locus_tag="ACHKUI_000240"
     CDS             complement(5196..6569)
                     /gene="dbpA"
                     /locus_tag="ACHKUI_000240"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO6596518.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(6599..6772)
                     /gene="ynaL"
                     /locus_tag="ACHKUI_000241"
     CDS             complement(6599..6772)
                     /gene="ynaL"
                     /locus_tag="ACHKUI_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6596519.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(7047..8030)
                     /gene="zntB"
                     /locus_tag="ACHKUI_000242"
     CDS             complement(7047..8030)
                     /gene="zntB"
                     /locus_tag="ACHKUI_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6596520.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(8044..8091)
                     /gene="ydaN"
                     /locus_tag="ACHKUI_000243"
     CDS             complement(8044..8091)
                     /gene="ydaN"
                     /locus_tag="ACHKUI_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6596521.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            8285..8530
                     /locus_tag="ACHKUI_000244"
                     /pseudo
     CDS             8285..8530
                     /locus_tag="ACHKUI_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            8690..9505
                     /locus_tag="ACHKUI_000245"
     CDS             8690..9505
                     /locus_tag="ACHKUI_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6596522.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            9855..10007
                     /locus_tag="ACHKUI_000246"
                     /pseudo
     CDS             9855..10007
                     /locus_tag="ACHKUI_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10018..10533
                     /gene="ogt"
                     /locus_tag="ACHKUI_000247"
     CDS             10018..10533
                     /gene="ogt"
                     /locus_tag="ACHKUI_000247"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6596523.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            10728..11480
                     /gene="fnr"
                     /locus_tag="ACHKUI_000248"
     CDS             10728..11480
                     /gene="fnr"
                     /locus_tag="ACHKUI_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6596524.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            11632..12582
                     /gene="uspE"
                     /locus_tag="ACHKUI_000249"
     CDS             11632..12582
                     /gene="uspE"
                     /locus_tag="ACHKUI_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6596525.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(12632..12889)
                     /gene="ynaJ"
                     /locus_tag="ACHKUI_000250"
     CDS             complement(12632..12889)
                     /gene="ynaJ"
                     /locus_tag="ACHKUI_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6596526.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            13133..14164
                     /gene="ynaI"
                     /locus_tag="ACHKUI_000251"
     CDS             13133..14164
                     /gene="ynaI"
                     /locus_tag="ACHKUI_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6596527.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(14215..15828)
                     /gene="mppA"
                     /locus_tag="ACHKUI_000252"
     CDS             complement(14215..15828)
                     /gene="mppA"
                     /locus_tag="ACHKUI_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6596528.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(16165..17064)
                     /gene="pgrR"
                     /locus_tag="ACHKUI_000253"
     CDS             complement(16165..17064)
                     /gene="pgrR"
                     /locus_tag="ACHKUI_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6596529.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            17202..18122
                     /gene="ycjY"
                     /locus_tag="ACHKUI_000254"
     CDS             17202..18122
                     /gene="ycjY"
                     /locus_tag="ACHKUI_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6596530.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTVGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            18193..18816
                     /locus_tag="ACHKUI_000255"
                     /pseudo
     CDS             18193..18816
                     /locus_tag="ACHKUI_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            19043..19771
                     /gene="mpaA"
                     /locus_tag="ACHKUI_000256"
     CDS             19043..19771
                     /gene="mpaA"
                     /locus_tag="ACHKUI_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6596531.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPTPATSHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19746..20711)
                     /gene="ycjG"
                     /locus_tag="ACHKUI_000257"
     CDS             complement(19746..20711)
                     /gene="ycjG"
                     /locus_tag="ACHKUI_000257"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6596532.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTRQELQKILPAGAARNALDCALWDLAARK
                     QQQSQADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            20830..21336
                     /gene="tpx"
                     /locus_tag="ACHKUI_000258"
     CDS             20830..21336
                     /gene="tpx"
                     /locus_tag="ACHKUI_000258"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6596533.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGVNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(21380..22921)
                     /gene="tyrR"
                     /locus_tag="ACHKUI_000259"
     CDS             complement(21380..22921)
                     /gene="tyrR"
                     /locus_tag="ACHKUI_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6596534.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(23069..24130)
                     /gene="ycjF"
                     /locus_tag="ACHKUI_000260"
     CDS             complement(23069..24130)
                     /gene="ycjF"
                     /locus_tag="ACHKUI_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6596535.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(24127..25524)
                     /gene="ycjX"
                     /locus_tag="ACHKUI_000261"
     CDS             complement(24127..25524)
                     /gene="ycjX"
                     /locus_tag="ACHKUI_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6596536.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            25681..26679
                     /gene="ycjW"
                     /locus_tag="ACHKUI_000262"
     CDS             25681..26679
                     /gene="ycjW"
                     /locus_tag="ACHKUI_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6596537.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTDALIESGHKNIACLHAPLDVHVSVDRVNGYRQSLAMHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(26730..27635)
                     /gene="ompG"
                     /locus_tag="ACHKUI_000263"
     CDS             complement(26730..27635)
                     /gene="ompG"
                     /locus_tag="ACHKUI_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6596538.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETLALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(27680..28762)
                     /locus_tag="ACHKUI_000264"
     CDS             complement(27680..28762)
                     /locus_tag="ACHKUI_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6596539.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(28776..29435)
                     /gene="pgmB"
                     /locus_tag="ACHKUI_000265"
     CDS             complement(28776..29435)
                     /gene="pgmB"
                     /locus_tag="ACHKUI_000265"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6596540.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(29432..31699)
                     /gene="ycjT"
                     /locus_tag="ACHKUI_000266"
     CDS             complement(29432..31699)
                     /gene="ycjT"
                     /locus_tag="ACHKUI_000266"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6596541.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLTGLYHRAGKGEINELVNLPDVVGMEIAINGEIISLSREAWQRELNFASGELR
                     RNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLPAGKTLTLQKRVWIDWRDDRHVALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLLGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELLLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDVQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGNF
                     ILPFNGTATTHQEGE"
     gene            complement(31696..32751)
                     /gene="ycjS"
                     /locus_tag="ACHKUI_000267"
     CDS             complement(31696..32751)
                     /gene="ycjS"
                     /locus_tag="ACHKUI_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6596542.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWSVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(32761..33549)
                     /gene="ycjR"
                     /locus_tag="ACHKUI_000268"
     CDS             complement(32761..33549)
                     /gene="ycjR"
                     /locus_tag="ACHKUI_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6596543.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGVKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWVRTC"
     gene            complement(33568..34620)
                     /gene="ycjQ"
                     /locus_tag="ACHKUI_000269"
     CDS             complement(33568..34620)
                     /gene="ycjQ"
                     /locus_tag="ACHKUI_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6596544.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILASEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(34651..35493)
                     /gene="ycjP"
                     /locus_tag="ACHKUI_000270"
     CDS             complement(34651..35493)
                     /gene="ycjP"
                     /locus_tag="ACHKUI_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6596545.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIIMLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(35480..36361)
                     /gene="ycjO"
                     /locus_tag="ACHKUI_000271"
     CDS             complement(35480..36361)
                     /gene="ycjO"
                     /locus_tag="ACHKUI_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6596546.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTTLVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVLLSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(36382..37674)
                     /gene="ycjN"
                     /locus_tag="ACHKUI_000272"
     CDS             complement(36382..37674)
                     /gene="ycjN"
                     /locus_tag="ACHKUI_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6596547.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKSEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQTSQKKLDELVEQR"
     gene            complement(37688..39367)
                     /gene="ycjM"
                     /locus_tag="ACHKUI_000273"
     CDS             complement(37688..39367)
                     /gene="ycjM"
                     /locus_tag="ACHKUI_000273"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO6596548.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDILLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYSIYYKLSRLITLRCSHNEFHPDNDFTIDTVNSSVMCIQRSN
                     ADGNCLTGLFNVSENIQHINITDLHGRDLISEVDIVGNEITLRPWQVMWIK"
     gene            complement(39577..39891)
                     /gene="pspE"
                     /locus_tag="ACHKUI_000274"
     CDS             complement(39577..39891)
                     /gene="pspE"
                     /locus_tag="ACHKUI_000274"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6596549.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(39966..40187)
                     /gene="pspD"
                     /locus_tag="ACHKUI_000275"
     CDS             complement(39966..40187)
                     /gene="pspD"
                     /locus_tag="ACHKUI_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6596550.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(40196..40555)
                     /gene="pspC"
                     /locus_tag="ACHKUI_000276"
     CDS             complement(40196..40555)
                     /gene="pspC"
                     /locus_tag="ACHKUI_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6596551.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMVFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(40555..40779)
                     /gene="pspB"
                     /locus_tag="ACHKUI_000277"
     CDS             complement(40555..40779)
                     /gene="pspB"
                     /locus_tag="ACHKUI_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6596552.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELPQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(40833..41501)
                     /gene="pspA"
                     /locus_tag="ACHKUI_000278"
     CDS             complement(40833..41501)
                     /gene="pspA"
                     /locus_tag="ACHKUI_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6596553.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            41668..42645
                     /gene="pspF"
                     /locus_tag="ACHKUI_000279"
     CDS             41668..42645
                     /gene="pspF"
                     /locus_tag="ACHKUI_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6596554.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCSALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            42831..42938
                     /locus_tag="ACHKUI_000280"
                     /pseudo
     CDS             42831..42938
                     /locus_tag="ACHKUI_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43072..43317
                     /gene="ymjA"
                     /locus_tag="ACHKUI_000281"
     CDS             43072..43317
                     /gene="ymjA"
                     /locus_tag="ACHKUI_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO6596555.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            43630..45273
                     /gene="sapA"
                     /locus_tag="ACHKUI_000282"
     CDS             43630..45273
                     /gene="sapA"
                     /locus_tag="ACHKUI_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6596556.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            45270..46235
                     /gene="sapB"
                     /locus_tag="ACHKUI_000283"
     CDS             45270..46235
                     /gene="sapB"
                     /locus_tag="ACHKUI_000283"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6596557.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            46222..47112
                     /gene="sapC"
                     /locus_tag="ACHKUI_000284"
     CDS             46222..47112
                     /gene="sapC"
                     /locus_tag="ACHKUI_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6596558.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            47112..48104
                     /gene="sapD"
                     /locus_tag="ACHKUI_000285"
     CDS             47112..48104
                     /gene="sapD"
                     /locus_tag="ACHKUI_000285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6596559.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            48106..48912
                     /gene="sapF"
                     /locus_tag="ACHKUI_000286"
     CDS             48106..48912
                     /gene="sapF"
                     /locus_tag="ACHKUI_000286"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6596560.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(48960..50123)
                     /locus_tag="ACHKUI_000287"
     CDS             complement(48960..50123)
                     /locus_tag="ACHKUI_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO6596561.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(50132..51505)
                     /locus_tag="ACHKUI_000288"
     CDS             complement(50132..51505)
                     /locus_tag="ACHKUI_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO6596562.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALFGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(51509..54616)
                     /locus_tag="ACHKUI_000289"
     CDS             complement(51509..54616)
                     /locus_tag="ACHKUI_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO6596563.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYVVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(54616..55743)
                     /locus_tag="ACHKUI_000290"
     CDS             complement(54616..55743)
                     /locus_tag="ACHKUI_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6596564.1"
                     /translation="MKYIATSVVAMLLLSGCDNTHTQSNNSSPSETEVGVVTLKSQPV
                     SVVSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEA
                     RAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALET
                     ARINLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLL
                     RLRKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQ
                     LLPGMFVRARVDEGVMENAILAPQQGVTRDAKGNANALVVNKDNKVEQRTLETGETYG
                     DKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(55892..56458)
                     /locus_tag="ACHKUI_000291"
     CDS             complement(55892..56458)
                     /locus_tag="ACHKUI_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6596565.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            56936..57724
                     /gene="fabI"
                     /locus_tag="ACHKUI_000292"
     CDS             56936..57724
                     /gene="fabI"
                     /locus_tag="ACHKUI_000292"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6596566.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            57869..58996
                     /gene="yciW"
                     /locus_tag="ACHKUI_000293"
     CDS             57869..58996
                     /gene="yciW"
                     /locus_tag="ACHKUI_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6596567.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIINNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            59064..60998
                     /gene="rnb"
                     /locus_tag="ACHKUI_000294"
     CDS             59064..60998
                     /gene="rnb"
                     /locus_tag="ACHKUI_000294"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6596568.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(61075..61178)
                     /locus_tag="ACHKUI_000295"
                     /pseudo
     CDS             complement(61075..61178)
                     /locus_tag="ACHKUI_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61232..63217
                     /gene="pdeR"
                     /locus_tag="ACHKUI_000296"
     CDS             61232..63217
                     /gene="pdeR"
                     /locus_tag="ACHKUI_000296"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6596569.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRT"
     gene            complement(63199..63327)
                     /gene="ymiD"
                     /locus_tag="ACHKUI_000297"
     CDS             complement(63199..63327)
                     /gene="ymiD"
                     /locus_tag="ACHKUI_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6596570.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYVRFR"
     gene            63365..63538
                     /gene="yciZ"
                     /locus_tag="ACHKUI_000298"
     CDS             63365..63538
                     /gene="yciZ"
                     /locus_tag="ACHKUI_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6596571.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            63628..64377
                     /gene="yciT"
                     /locus_tag="ACHKUI_000299"
     CDS             63628..64377
                     /gene="yciT"
                     /locus_tag="ACHKUI_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6596572.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            64646..64864
                     /gene="osmB"
                     /locus_tag="ACHKUI_000300"
     CDS             64646..64864
                     /gene="osmB"
                     /locus_tag="ACHKUI_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6596573.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(64990..65316)
                     /gene="yciH"
                     /locus_tag="ACHKUI_000301"
     CDS             complement(64990..65316)
                     /gene="yciH"
                     /locus_tag="ACHKUI_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6596574.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVALDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(65316..66053)
                     /gene="pyrF"
                     /locus_tag="ACHKUI_000302"
     CDS             complement(65316..66053)
                     /gene="pyrF"
                     /locus_tag="ACHKUI_000302"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6596575.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(66245..67414)
                     /gene="lapB"
                     /locus_tag="ACHKUI_000303"
     CDS             complement(66245..67414)
                     /gene="lapB"
                     /locus_tag="ACHKUI_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6596576.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(67421..67729)
                     /gene="lapA"
                     /locus_tag="ACHKUI_000304"
     CDS             complement(67421..67729)
                     /gene="lapA"
                     /locus_tag="ACHKUI_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6596577.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            complement(67878..68642)
                     /gene="pgpB"
                     /locus_tag="ACHKUI_000305"
     CDS             complement(67878..68642)
                     /gene="pgpB"
                     /locus_tag="ACHKUI_000305"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6596578.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWSRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            68812..69402
                     /gene="ribA"
                     /locus_tag="ACHKUI_000306"
     CDS             68812..69402
                     /gene="ribA"
                     /locus_tag="ACHKUI_000306"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6596579.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(69466..72141)
                     /gene="acnA"
                     /locus_tag="ACHKUI_000307"
     CDS             complement(69466..72141)
                     /gene="acnA"
                     /locus_tag="ACHKUI_000307"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6596580.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNSGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTC
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELAYYQNDGILHYVIRNMLK"
     gene            complement(72291..72386)
                     /gene="ymiC"
                     /locus_tag="ACHKUI_000308"
     CDS             complement(72291..72386)
                     /gene="ymiC"
                     /locus_tag="ACHKUI_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6596581.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFCAELLWIITH"
     gene            complement(72500..72667)
                     /gene="yciX"
                     /locus_tag="ACHKUI_000309"
     CDS             complement(72500..72667)
                     /gene="yciX"
                     /locus_tag="ACHKUI_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6596582.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(72670..72810)
                     /gene="ymiA"
                     /locus_tag="ACHKUI_000310"
     CDS             complement(72670..72810)
                     /gene="ymiA"
                     /locus_tag="ACHKUI_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6596583.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVVVALLIWKL
                     WG"
     gene            complement(73129..74103)
                     /gene="cysB"
                     /locus_tag="ACHKUI_000311"
     CDS             complement(73129..74103)
                     /gene="cysB"
                     /locus_tag="ACHKUI_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6596584.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(74313..76910)
                     /gene="topA"
                     /locus_tag="ACHKUI_000312"
     CDS             complement(74313..76910)
                     /gene="topA"
                     /locus_tag="ACHKUI_000312"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6596585.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRGRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            77290..77541
                     /gene="yciN"
                     /locus_tag="ACHKUI_000313"
     CDS             77290..77541
                     /gene="yciN"
                     /locus_tag="ACHKUI_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6596586.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(77577..78626)
                     /gene="sohB"
                     /locus_tag="ACHKUI_000314"
     CDS             complement(77577..78626)
                     /gene="sohB"
                     /locus_tag="ACHKUI_000314"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6596587.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKSLM"
     gene            78846..79604
                     /gene="yciK"
                     /locus_tag="ACHKUI_000315"
     CDS             78846..79604
                     /gene="yciK"
                     /locus_tag="ACHKUI_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6596588.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEEIGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            79601..80191
                     /gene="cobO"
                     /locus_tag="ACHKUI_000316"
     CDS             79601..80191
                     /gene="cobO"
                     /locus_tag="ACHKUI_000316"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6596589.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(80231..81106)
                     /gene="rluB"
                     /locus_tag="ACHKUI_000317"
     CDS             complement(80231..81106)
                     /gene="rluB"
                     /locus_tag="ACHKUI_000317"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6596590.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(81319..83208)
                     /gene="yciQ"
                     /locus_tag="ACHKUI_000318"
     CDS             complement(81319..83208)
                     /gene="yciQ"
                     /locus_tag="ACHKUI_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO6596591.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLTFLFCSLDLLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGVYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            complement(83236..83856)
                     /gene="yciO"
                     /locus_tag="ACHKUI_000319"
     CDS             complement(83236..83856)
                     /gene="yciO"
                     /locus_tag="ACHKUI_000319"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6596592.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(83853..84734)
                     /gene="rnm"
                     /locus_tag="ACHKUI_000320"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(83853..84734)
                     /gene="rnm"
                     /locus_tag="ACHKUI_000320"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6596593.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALESAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            84872..84916
                     /gene="trpL"
                     /locus_tag="ACHKUI_000321"
     CDS             84872..84916
                     /gene="trpL"
                     /locus_tag="ACHKUI_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6596594.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    84893..84986
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            85008..86570
                     /gene="trpE"
                     /locus_tag="ACHKUI_000322"
     CDS             85008..86570
                     /gene="trpE"
                     /locus_tag="ACHKUI_000322"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6596595.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGYLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            86570..88165
                     /gene="trpD"
                     /locus_tag="ACHKUI_000323"
     CDS             86570..88165
                     /gene="trpD"
                     /locus_tag="ACHKUI_000323"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6596596.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLKPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            88169..89527
                     /gene="trpCF"
                     /locus_tag="ACHKUI_000324"
     CDS             88169..89527
                     /gene="trpCF"
                     /locus_tag="ACHKUI_000324"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6596597.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFTNGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTREQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            89539..90732
                     /gene="trpB"
                     /locus_tag="ACHKUI_000325"
     CDS             89539..90732
                     /gene="trpB"
                     /locus_tag="ACHKUI_000325"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6596598.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            90732..91538
                     /gene="trpA"
                     /locus_tag="ACHKUI_000326"
     CDS             90732..91538
                     /gene="trpA"
                     /locus_tag="ACHKUI_000326"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6596599.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEVLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(91589..91729)
                     /locus_tag="ACHKUI_000327"
                     /pseudo
     CDS             complement(91589..91729)
                     /locus_tag="ACHKUI_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            91924..92001
                     /locus_tag="ACHKUI_000328"
                     /pseudo
     CDS             91924..92001
                     /locus_tag="ACHKUI_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261927.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
     gene            complement(92177..92629)
                     /locus_tag="ACHKUI_000329"
     CDS             complement(92177..92629)
                     /locus_tag="ACHKUI_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapA/VapB family virulence-associated protein"
                     /protein_id="HFO6596600.1"
                     /translation="MVTTMTKCSNLRQQIMDDVQRRYGEYLDKDKVSCITSKIAAAEN
                     KYPAKTTLASAIFYIKVDTQITSEGGKHFSGNAGGLSSPGGGVLFGDLYTDDLDDLYT
                     NTVSFQITMTPVFCSVLFFDSASNLLGHFEGGGVSTVSGVAGGTGSWS"
BASE COUNT        23380 a        23976 c        22064 g        23228 t
ORIGIN      
        1 atgatattta tcggctaact gaacttctcc tttaggatgt tttcactccc caaaatgggg
       61 atgaaaggca aataaaataa ctaaaggatt tattcaatga aaagcaaagt actggcactc
      121 ttaattcctg ccctgctcgc tacaggtgct gcacatgcag ccgaagttta taataaagac
      181 ggcaacaaat tagatctgta tggcaaagtt gatggcctgc attatttttc tgataattca
      241 gcgaaagatg gcgaccagag ctatgctcgt ctgggtttta aaggcgaaac ccaaattaac
      301 gatcaactca ctggctacgg tcaatgggaa tacaatattc aggcaaacaa caccgaatct
      361 tcaacaaacc agtcatggac ccgtctggca tttgccggac tgaaatttgc agattacggt
      421 tctttcgatt acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gactgatatg
      481 ctacctgaat ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgtgcc
      541 aatggcgtcg cgacttatcg taatgctgat ttcttcggtc tggtaaatgg tctgaacttc
      601 gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg gtcaggaagg caccaacaac
      661 ggacgtgatg ttcgccatga aaacggtgac ggctggggtc tttccacaac atatgattta
      721 ggcatgggct ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgaccaggtt
      781 aaccatactg cggcaggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat
      841 gctaacaata tttacctggc aaccatgtat tcagaaacgc gtaatatgac cccgtttggc
      901 gacagcgatt atgctgttgc aaacaaaacc cagaactttg aagtcactgc acagtaccag
      961 tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg tgacctgcac
     1021 gctgccggtg gtgcagacaa cccagcaggt gttgatgata aagatctggt taaatacgcc
     1081 gatgttggcg cgacttacta tttcaataaa aacatgtcca cctatgttga ctataaaatc
     1141 aacctgttgg atgaagatga cagtttctac gctgccaacg gcatctctac cgatgatatt
     1201 gtcgctttag gtctggttta tcagttctaa atccccctgc ccgctgtttt ggcgggcttt
     1261 ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa
     1321 aagtgtcatc tggcgttaca ctttatgcgg atactaaaac aggaggtttt atgaacagaa
     1381 cgattcttgt ccctatcgat atttccgatt cagaattaac tcaacgcgtg attagccacg
     1441 ttgaggcaga ggcaaagatt gatgatgcag aggttcattt cctgacggta ataccttcgc
     1501 tgccctacta tgcctctctg ggtttagcct attccgcaga attaccggca atggatgatc
     1561 tgaaagcgga agccaaatcg caactggaag agatcattaa aaaatttaaa ctgccaaccg
     1621 acagagtgca tgtccatgtt gaagaaggct cgcccaaaga ccgcattctg gaattggcga
     1681 agaagatccc cgctcatatg atcatcattg cttcccatcg accggatatc accacttatc
     1741 tgctcggttc taacgccgca gctgtagtgc gtcacgcaga gtgctccgtg ctggttgtgc
     1801 gctgacacta acgcccgcac attgctgcgg gctttttgat tcatttcgca aatgtgctga
     1861 cattttcccc tctaatcatt acaatttaaa tttatgaatt atgcattgat aatcgctatt
     1921 ataatatcgt tcccctgtgc attattatca gtttttctgg tattaaaaga ctgggcgcta
     1981 ataggagatg caatgaacca cgcaatgttt cctggcatag ttctatgcct ggattatagg
     2041 tatgccgctg ggaattggtg catttattgt aggactgtct tgtgcaattg caactggcta
     2101 tctgaaagat aatagcatca taaagaaaga taccattata ggtatcgttt tttcaggaat
     2161 ttttgtcttg actatcaaca ggcacaattc agcggattac agatatcatt attacattac
     2221 ggtttactct gcatggtatc gcctaccatt tttgccacat taaaagcagt cggaattatt
     2281 ctgtctatat actattaata gctccagggt ctattgctgt cctcatcacg catcgctttc
     2341 ataagacatt acttatcgcc gtaacgatat caataacaat atcaggcgta tggttattat
     2401 attttctcga cagagcccca gcgccgacaa tcgtcctctt atttgctgta gcatttcttt
     2461 tcgcgcttat aataatcaat tttcggaaac aataatctca acagtagaat aaagaggcaa
     2521 atcaatcatt acatatataa gatggtgcta atattgcacc gcctttaaat tgaactttat
     2581 cctttcaccc ttacacaatt taaggaatgc agatgactat tttatcagca cccagccttt
     2641 ttcctggtaa tcgaaaactt gaaaatcatt aactctaaga aattctgcca aatcgttgaa
     2701 attactttaa attagactcg aacaaatcaa tccctgatgg gacttttact tatcaattta
     2761 atcgtgtaga tatcgacctc catctgcccg cctcagattc gtacctggtg gaggtctttt
     2821 ttcgttacct ctttccccct ttagttcagt ataaaatcat caaactaatg ctgttcacac
     2881 tatttacctc cagcattcta tataaataat agaaataaaa accccaatac atagtgcatt
     2941 ggcatgacca aacatcaatg ccattatact cgcctctgtt aaatactcgc tagttaatat
     3001 gataggcacg catgatataa caatccatat gctaatacat aaatacatca gtatttttct
     3061 catagtcagt ccctgtgatt aacaatcaag gcatagttaa gcatatttca cattcaaacg
     3121 taaacattat taataacatt atgtcaatcg aactgacatc acgattcaag ccctcttcag
     3181 actgaaggtt agagcagcgt agaacatttc aaaacaatac actaaaaaaa tacttgaaca
     3241 aaaaacttaa ttctttttta ttgtgaactt tagtaatttt gtcatgcaac tctttattac
     3301 atttaggcct atcttatttc atggcaacgc agatacagga ccatttcttg tgctatggat
     3361 aagtaataag ctagttacat cttattaaga gaaccatttt tacatatcaa gatatcatca
     3421 ccacccacaa tgaattaagt atttttctta ttctttagat tagatacgta atgaatccct
     3481 aactccattc gggtgtcatg gttctttcat ttgaaataca ttcatatcca atgtaatatt
     3541 tccatgcgca aatataacca ccaaccatac ccatcattta ttacagagaa ttattttatt
     3601 caccgaaaag tttcacaaaa tatcccctga tagaaaacta aaaaacactg cataatatcg
     3661 ctattctggc acttgcgaaa aattatattc tgcacttgtt gtagcgttct atttctccgc
     3721 ctggcaacga atgcacaatg tccgccacag tttctgttct aatttcttcc tgtgttaccg
     3781 catcctgtac gggtagcgca acaccagcat gctaagcaag gctttcccat cagatttcac
     3841 tacgactatt tgggagcgag atggaatgca gcagtaaaac gtgctggtat tcgccgccgt
     3901 aatccgtacc atacgcgaca tacttttgcc tgctggctgt tgacggcagg agcgaacccg
     3961 gcatttatag ccagccagat ggggcatgaa actgcgcaga tggtgtatga aatttacggt
     4021 atgtggattg atgacatgaa cgacgaacag gtagccatgt tgaatgcgcg gttatcgtag
     4081 ttgcaaagtt tgccctcaat atgccacatt tagtaccaga gaactgaaat aatgcaagaa
     4141 aatcaacaaa ttacaaagaa agaacaatac aacctgaaca aattacaaaa acgtctgcgt
     4201 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
     4261 gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag
     4321 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
     4381 ttcccggaac acgttctgcc cgagtatctt gaaacgctgg gcgttgagta caagattgtt
     4441 gaagagaaca cttacggtat cgtgaaagag aagattccgg agggcaaaac cacttgctca
     4501 ctgtgttctc gtcttcgtcg cggtatcctt tatcgcaccg caacggaact gggggcgacg
     4561 aagatcgcgc tgggtcacca tcgtgacgat atcctgcaaa cgctgttctt aaatatgttc
     4621 tacggtggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
     4681 gtgattcgtc cgctggccta ctgccgcgag aaagatattc agcgctttgc cgatgcaaaa
     4741 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
     4801 attgctgaca tgttgcgtga ctgggataaa cgttatccgg ggcgtatcga gacgatgttc
     4861 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa
     4921 ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
     4981 atcccactac aaccagcggg ctggcagcct gaagaagatg aaaatcagtt ggatgagtta
     5041 cggctgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg
     5101 ctactcttat ggaggccgga gaagacgcgg ccagcgtcac atccggcaat cccgaagaag
     5161 atgtttactc ttgcaccctg caattcaaca tttcattatt ttaataaccg cacccggcac
     5221 gtttttcctt taatcttccc gccctgtagc tgtttccatg ctttatgagc aacagcctga
     5281 cgcaccgcga cataaacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
     5341 agcccgatgt ctcctgtcag cgcacctaat acatcacccg ggcgcatttt ggcttttttc
     5401 ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga actattcgct
     5461 ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
     5521 gcttcttccg gggcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga
     5581 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
     5641 agcgatttta tatccagacc acgcgcagca acatcagtcg cgaccaatac acgggcgcta
     5701 ccgttagcaa aacgtaccag ggtctgatcg cgatcgcgtt gctccagatc gccgtgtaac
     5761 gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagactggca atcttttttg
     5821 gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga
     5881 ggaattttgc ctttgctgga tgtctcataa aattgttgtt caataggtgg cagagcatct
     5941 gttgagtcaa tttcaatcgc taaaggatcg cgttgcactc ttccgctgat tgcggcaatg
     6001 gcttccggcc aggttgccga aaacagaagt gtctgccgag atgctggcgc aaaacagatg
     6061 acatcatcaa tggcgtcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc
     6121 agcgtattca acgcatccag tgatacagtg cctttttgca ggtgatccag caaacggccc
     6181 ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt
     6241 tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat
     6301 tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga
     6361 aatagcgaca catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg
     6421 ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc
     6481 tgcaccggcg tcatggttaa ataacccaac tcattaaggt ttgtgagttg ggcgggaggc
     6541 aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtc
     6601 agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat
     6661 cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggtact ggaacaggat
     6721 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
     6781 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
     6841 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
     6901 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagagagat
     6961 attgccctga atgggtagag agtttattga cttcgctcaa actttacggc gtttttgtat
     7021 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
     7081 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccaaa ttgccatccg
     7141 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaacgt actgggtaaa
     7201 aagaccattg ccatcaacga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
     7261 tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaaga
     7321 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
     7381 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
     7441 atcacaatta attgtttgcg cagcagagcc agaaatccac gcggtggaat ttgctgatcg
     7501 agaagattat cttcgaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
     7561 tgatcggtca acgcatcgca cacatccacc agccaccccc cgcaatcggt cggaccagtg
     7621 ccctcttcaa gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
     7681 acaattaacc gcccgtccat atatacacgc atagcgacca gttgatcggg gcgttcatcg
     7741 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
     7801 ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
     7861 gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca gggatgcgct
     7921 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
     7981 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
     8041 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
     8101 ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa gcaaaatgct
     8161 tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag aattatctga
     8221 tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa ataatgggat
     8281 ctcaatgatt acacacaact tcaataccct ggacttactc accagtcctg tctggatcgt
     8341 ttcgcccttt gaggaacagt taatttatgc caacagcgcg gcgcgactgt tgatgcaaga
     8401 cctcacgttt agtcagctac gaaccggtcc ctattccgtc tcctcacaaa aagaactgcc
     8461 gaaatacctc tccgatctgc aaaatcaaca cgatattatc gaaatcctca ccgttcagcg
     8521 taaagaagac aacgccagcc aaaataatac cgatcgctac caccccaata agctgttttt
     8581 tagaaattat catcactctt tacaccgtat atatccggaa acttgcatac gtcatattta
     8641 aaatttaaca tggcttattt gatgatttct gacaaaatac ctactgtaaa tgacaatcac
     8701 actgcaaccc aaagcatcac cagggcatca cattatcgga ctggattcgg aacatctgaa
     8761 tggaggcaca gttccctggc ataaacatct ctatgcccag ttgctttacc cggctgaagg
     8821 agtcgttcgt atatgggctg gagaaagcgt ctggctagtg catgccagca gcgcgctttg
     8881 gctgccccca caaatgccgc ataagtttgt tgcgacaggc aacgtacttt taaaaaccgt
     8941 actggtttca gaagcggaaa gtgaaacgct gggaaaagtt tgcttcatga cgggtatttc
     9001 acccctgtta agggaattac taattgcaat taaccaactt ccaccgtcgc aaagtaccac
     9061 agataaacaa cagttacgtt tttcagccct cgagacactg atacttcagg aaataaaaat
     9121 gggggtgaaa atgtcacttg aactcccttg gccaaatgat gagcggctac agcagctttg
     9181 tgagaatttg ctaaacaacc aagggtatct gccaacactg gataatctag cggataaaat
     9241 taacgtcagt agccgtacac tcatgcgctt atttgttaaa gagacgggat taacatttcg
     9301 ccactgggtt cagcaaatgc acgttatttc tgcagtgacg cttcttgatg atggatattc
     9361 gttgaccaaa attgcccatc gtctgggcta tgcctcagct gaatcgttcg gcaatatgtt
     9421 taagcgtcga acgggctact cacccggtaa atttactcgt cgattaacga tgcatgatta
     9481 tgcaatcaca agacaaatga tttaacttat attttcgtga aatctgtcac tgaagaaaat
     9541 tggcaacgga tgggtaaaac cgttataaca ctgtcaccgg tcaccaggac cccaggccgg
     9601 atttaagacg aggatgcact gctgtgtgta ctgtagagtc tggtggatgt cgacaggctc
     9661 tattttttta ttcatttttt agctctgtaa ataaccacat tctcgccggg attggggaat
     9721 gagtgacgag atcagtggtg tctggcggct atcacgccac agaatcaaat aacggatagc
     9781 ctacggtaaa atttcgctga ctggaatttg atggcttgcc ctcatttctt caggcagaaa
     9841 aatcaaaaaa tcgctttatg cttaaatact tcgacaaacc agacgctaaa cctggtactt
     9901 actttttgtt agtattaatc ttttcggtgg tatggctgct gatgttgctg gcgtggtatc
     9961 ttgttggctt gccgataggt ccgggtattt acccacgttt gtcttaagag agaacggatg
    10021 ctgagattac ttgaagaaaa aattgccacg ccactgggtc cactgtgggt gatttgcgat
    10081 gagcaatttc gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat ggtgcagctg
    10141 ctggacatcc attatcgcaa agaaagctat gagcgcattt ctgccaccaa cccaggtggt
    10201 ttaagcgaca agcttcgtga atattttgcc ggtaatctta gcattattga tacgcttccc
    10261 actgccacgg gggggacgcc atttcagcgc gaagtctgga aaacactgcg cactatcccc
    10321 tgcgggcagg taatgcatta tggccaactg gctgaacaat taggccgccc tggcgcggcg
    10381 cgtgccgttg gtgcggcaaa cggatcgaat cccatcagca tcgtcgtacc ttgccatcgg
    10441 gttattggcc gaaacggcac catgaccgga tatgcaggcg gagttcagcg aaaagagtgg
    10501 ttattgcgcc acgaaggtta tcttttgctg taaacattaa acaatttgtg ccagcttgtt
    10561 cacactttta tgtaaagtca cccttaacaa cttaagggtt ttcaaataga tagacatata
    10621 tttacatcta atatcggaat tctctgctgt taaggtttgc ttagacttac ttgctcccta
    10681 aaaagatgtt aaaattgaca aatatcaatt acggcttgag cagacctatg atcccggaaa
    10741 agcgaattat acggcgcatt cagtctggcg gttgtgctat ccattgccag gattgcagca
    10801 tcagccagct ttgcatcccg ttcacactca acgaacatga gcttgatcag cttgataata
    10861 tcattgagcg gaagaagcct attcagaaag gccagacgct gtttaaggct ggagatgaac
    10921 taaaatcgct ttatgccatc cgctccggta cgattaaaag ttataccatc actgagcaag
    10981 gcgacgagca aatcactggt ttccatttag caggcgatct ggtgggattt gatgccatcg
    11041 gcagcggtca tcacccgagt ttcgcgcagg cgctggaaac ctcgatggta tgtgaaatcc
    11101 cgttcgaaac gctggacgat ttgtctggta aaatgccgaa tctgcgtcag cagatgatgc
    11161 gtctgatgag cggtgaaatc aaaggcgatc aggacatgat cctgctgttg tcgaagaaaa
    11221 atgccgagga acgtctggct gcattcatct acaacctgtc ccgtcgtttt gcccaacgcg
    11281 gcttctcccc tcgtgaattc cgcctgacga tgactcgtgg tgatatcggt aactatctgg
    11341 gcctgacggt tgaaaccatc agccgtctgc tgggtcgctt ccagaaaagc ggtatgctgg
    11401 cagtcaaagg taaatacatc actatcgaaa ataacgatgc gctggcccag cttgctggtc
    11461 atacgcgtaa cgttgcctga ttcttccgca taactcacta tccttctgtc atatcattaa
    11521 atttttctga tttattgatc tgacagaagg ttcatcactg tttcattcac cagatatggg
    11581 ttaatctttt aattacaaac tgcgttgaca gttgttgtaa ggagaccctg tatggctatg
    11641 tatcagaaca tgctcgttgt tatcgatcct aaccaggacg accaaccagc attgcggcga
    11701 gctgtttatt tacatcaacg gattggtggc aaaattaaag cctttttgcc gatctatgac
    11761 ttctcatacg aaatgaccac cctgctctct ccggacgaac gtaccgctat gcgtcagggc
    11821 gtcatcagcc agcgtacagc ctggatccac gagcaggcaa aatattatct caatgctggc
    11881 gttcccattg aaattaaagt ggtctggcat aaccgtcctt tcgaagccat cattcaggaa
    11941 gtgatcagcg gcgggcacga tttggtgcta aaaatggcgc accaacatga ccgtctggaa
    12001 gcggtgattt ttacgccaac ggactggcat ctgttacgca aatgcccaag cccggtgtgg
    12061 atggtgaaag accagccgtg gccggaaggg ggtaaggcgc tggtggcggt gaatctcgcc
    12121 agtgaagagc cgtaccataa tgcgctcaat gaaaaactgg tcaaagagac gatcgaactg
    12181 gcagaacaag tcaaccatac cgaagttcat ctggttggcg cttatccggt aacgccaatt
    12241 aatatcgcga ttgaactgcc ggaatttgac ccgagcgttt ataacgatgc cattcgtggg
    12301 caacatttgc tggcaatgaa agctctgcgg cagaaattcg gcattaatga aaacatgacg
    12361 cacgtagaaa aaggtctgcc cgaagaggtg attcctgatt tggcggagca cttacaggcg
    12421 gggattgtgg ttctgggcac ggtcggacgc accggtattt cagcagcatt ccttggcaac
    12481 acggcggaac aggtgattga tcatcttcgc tgcgacctgc tggtcattaa acctgaccag
    12541 tatcagacac ccgttgaact ggatgacgaa gaagacgatt aacgactctt caataactga
    12601 caaggcccga gttatcgggc ctatttttta cttactgctc ttcgccgcca aggaaataaa
    12661 ttcccaacgg gattgccagc aacacagtaa agaccaggct ataaacaaaa atcatcgatg
    12721 actgaatcac atacattgat gtcgtccact cagacagcgg aatattgtac tgttctataa
    12781 cgccgccgat ggtcgcgcga gtcacaaccg acgccgcaat aatgaaaacc gccaacaaac
    12841 caaagagaaa tttcttccct ttcgctgact tcagtttcgc cataatcatg atgtaatcct
    12901 tgtgcagata aatttcttgt tacctaaacg taacaatacc atgacatttc accgttgtct
    12961 gctctgaaaa tctcacctct tagctggatt tacaatcaac gcattgatta gttagctaat
    13021 gttgctactt ccttccctga ctattgatga tctgaatgaa ttgtcgcggt aaaatagtcc
    13081 cgccaggggt ttccggcatg acgacaatta tctggaggag cctgataaaa caatgatcgc
    13141 tgaactgttt acaaataatg cgcttaatct ggtcattatt ttcggtagct gcgcagcatt
    13201 gattctgatg agcttttggt ttcgccgtgg aaatcgtaaa agaaaaggat ttttattcca
    13261 tgcggtgcaa tttttaatct acaccataat tattagtgct gttggtagca tcattaacta
    13321 tgttatagaa aactataaac tcaaatttat caccccaggc gttatcgatt ttatctgtac
    13381 gtccctgatt gcggttattc tgacgattaa gctatttctg ctgattaatc agtttgaaaa
    13441 acagcagatt aaaaaaggtc gcgatatcac cagtgcgcgg atcatgtcgc gcattatcaa
    13501 aatcaccatt attgtggtgc ttgttctgct ttatggcgaa catttcggca tgagcctttc
    13561 tggcttgttg acctttggtg gtattggtgg tctggctgtc ggtatggccg gtaaagatat
    13621 tctgagtaac ttcttttccg ggattatgct ctatttcgac cgtcctttca gtattggcga
    13681 ctggatccgt tcaccggaca gaaatatcga aggtacagta acggaaattg gctggcgaat
    13741 taccaaaatt aagacctttg ataatcgtcc attgtacgta ccgaactcgc tgttttcgtc
    13801 gatcagcgta gaaaatcctg gacgaatgac caaccggcgc attaccacga ccattggttt
    13861 acgttatgag gatgcggcaa aagtgggcgt tattgtcgaa gccgtacgtg agatgctgaa
    13921 aaatcacccg gccatcgacc agcgacaaac cttactggtt tatttcaacc agtttgctga
    13981 ttcgtcatta aatattatgg tttattgctt taccaaaacc acggtatggg ctgagtggct
    14041 tgccgcacag caagacgttt atttgaagat tatcgatatt gtacagtcac acggcgcgga
    14101 ctttgccttc cccagccaga cgctgtatat ggataacatt acaccgccgg accagggtcg
    14161 ctaaatcaga catcactggc gttatccaca agggtaacgc cagttcccat cacattaatg
    14221 cttcacaata tacatagtcc gactgtacgc cacatcttca gggttattaa tgggataacc
    14281 tttcagccac ggcttgatta atcgtccatt ggtatattga taaattggtg caatcggtgc
    14341 ttgctccatg aggatttttt ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt
    14401 ttccgtcgat gcctgggcca gaactttgtc atatgccgga ttgttaaagc gtgaaatatt
    14461 cccggaatgc gttgacgtta ataatgtcag gaaagtggag ggttcattat aatcccctac
    14521 ccacgaggcg cggataacat caaaattgcc ggtattacgg ctatcgatat aggttttcca
    14581 ttcctggttt tgcaatttaa catctacgcc caggttcttt ttccacatcg atgctacagc
    14641 aatcgcaatt ttttgatggt tttctgaagt gttatacaaa agcgtcaact tcagcggttt
    14701 ttgcggacca taaccagctg cgctcaacaa agtttttgcc tgcgcattca gttcttcctg
    14761 actcatttgt tcaaacggcg aaggttccgg cgtaaatccc gcggtaacat ctggtgtaaa
    14821 atgccacgct ggcttttcgc ccgtccccaa cactttttcg gtcatcaggc ggcgatctat
    14881 cgtcatactt aatgccaggc gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa
    14941 cgcataataa taggtcccga gctgcggcgg cgtataaacc tgccccggaa tatccttcaa
    15001 caacttctga tacatatttt tcgggaagga ttcggtgata tcaatatccc ccgcgagata
    15061 acgcttagtg gctgcggatt cctgattaat tggcaggaag gtcacttttt gcagtaccgt
    15121 tttggcgtta tcccaataat gggtattcgg cactacgacc agtttttcat tgactacgcg
    15181 ctctttaaga acataagcgc cattgccgat cagatttccg ggtttcgtcc actctttact
    15241 gctttctacg ttggcttttt gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa
    15301 ccacggcaat ggtttatcaa gctggatttt caaagtatgg gcatcaactg cggtgacgcc
    15361 aagctggtca ggcgcggctt taccatcaat aatcgcctgt gcgttgttga ttcccgccag
    15421 cgcggcaaac catgcaaatg gcgatagcgt tttcgggtcc accagacgtt gccagctgta
    15481 gacaaaatct tgcgccgtta ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt
    15541 aaaagtccag atacggttgt cattactttt ccactgagtc gcaacgccgg ggacaatctc
    15601 ccctttctcg ttctgattca ccagtccttc aaacagatcg cgaatgacct gaatctccgg
    15661 caggcccacg gctttagcgg gatccagcga cgctggctca tctttaatgt ggcgcaccag
    15721 ctcctgcttc tctgccagta ctgtgccgct cggaacttca gcagcatacg aaagagaaat
    15781 gctgctgacc aacagcgcac aacacgtgac tgaaacagag tgcttcataa gtttccctct
    15841 aaccgacatt aaaattgatg cgtaattatt tgtttcgctt taaagaaatg caaacaactt
    15901 ccatcaaaaa agtgacaaag gaagcatcaa atgttttaag tatcaatata tcaaatcaga
    15961 tttagtgtaa ataatagtct ccaaaatgcg gtaaagcaca tagaactccg aacactacgt
    16021 tcgggcttcg cacattcaaa cccgttttcg gtatcataat cagcatctgt cgccattgtg
    16081 cgaagaataa aaaataacca aaacgttgaa aggaactgag aatacgttaa gagaagaatt
    16141 aaattaatgt atcagcaagt agtgctaaac agcaccttta tacttcagcc tatctataaa
    16201 taatgagaat gccgagccag catgtcgacg gtgtggatag tacaggtgat aaccgggtaa
    16261 atctggtgtg aatttatcca aaacacgtat cagttttttt tctttaatag cccgctcaac
    16321 ctgatcataa ggtagatacg ccaatccatg cccatcaatt gcagcatcaa tgatcaggtc
    16381 tatcgtattc agtaaaagct gaccttccat gcgaacacga acttcacgtc caccgcgtat
    16441 taatctccag cgatttgctg tacccgatgt gggaagatac aaattaattg cctgatgatc
    16501 tattaattgt gacactgacg ttggagcact tcgacgagaa aaataatccg gtgagccaac
    16561 aatagccatt ggtatgtctg gcccgattcg aatggcgatc atatctttat ccatctcccc
    16621 acccagacgg acgcctgcat caaaacgttc agaaacgaca tcggtcaaac cataatcaat
    16681 ggtaagctga atatcaattt caggatgcga tttcaggaat gtgcgcatag ctggtaacaa
    16741 tatcgttttt gctgcatgtt ctacagtagt aatacgtatt gtcccggatg ggcggttctg
    16801 cagatcgctc agggatgtca gggcagaatc tatgtcatgc aacatagggc caagaacaga
    16861 caaaagatgc tcgcccgctt cagtgggtac aacgctgcgc gtggttcgcg tcagaagccg
    16921 caatcccaat cgttcttcta tacggcgcac tatctggctt aaagctgact gcgccatgct
    16981 caggcgggct gctgcgcgag tgaagctacg ctcctctgca acaacgacaa acgccatcag
    17041 atcagcgatt tcttcacgct tcattcagta tctccataca atctattaat catttaaatg
    17101 ataggtctaa tcatgatttt gggtctaatc aacttaatga tcaatatcta tagtgatatc
    17161 accaaccgtt aaaacgaacc gacaactcca tgaggaacgg aatgatgaat aataaagtca
    17221 gcttcactaa cagcaataat ccaaccatct ctttgtctgc agtgatctat ttccctccaa
    17281 aatttgatga aacccgtcag tatccggcaa ttgtggtatc tcacccaggt gggggcgtta
    17341 aagagcaaac ggtcggaacc tatgccgaaa aactggcgga aaagggattt gtcactattg
    17401 cttatgacgc atcttatcaa ggtgaaagcg gcggcgagcc gcgtcagtta gaaaacccat
    17461 acattcgtac tgaagacgtc agtgccgtga ttgattacct gacaacgctt tcttatgtcg
    17521 acaatacccg aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg
    17581 caattcagga tcgccgcatc aaggccatcg gtactgttag tgcggtcaat attggttcga
    17641 tgttccgtaa tggttgggaa aacaatgtga aatctattga tgccctacca tacgttgacg
    17701 cgggttcaaa cgcccgaacc agcgatatta gcagcggaga atacgccact atgccactgg
    17761 caccaatgaa ggagtccgat gcgccgaacg aagaactgcg tcaggcctgg gaatattacc
    17821 acacccctcg cgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc
    17881 agattattac ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatacaga
    17941 ttgtggcggg cagccaggct gggagcaaat ggatgagtga cgacctgtac gatcgagcat
    18001 caagccagga taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgatg
    18061 gcaaagtcta tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt
    18121 aatgaaacat attcaaatcc gtaatagtga tatggcctgg catattgctg ccaataatct
    18181 gggaaaagtt tcatgaaaaa tgtactgatt cttggtgctg gcggtcagat agcccgccat
    18241 gtgattaatc aactggcaga taaacaaacc attaaacaaa ctttatttgc ccgtcagcca
    18301 gcaaaaatcc acaaacccta tcccactaat agcaagatta ttatggggga tgtactgaat
    18361 catgcagcac tgaagcaagc catgcaggga caggatgttg tgtatgccaa ccttacgggg
    18421 gaagatttag atattcaggc aaattcagtg atcgctgcga tgaaagcctg tgatgttaaa
    18481 cgtctgatct ttgttctttc cctcggtatt tatgatgaag ttccaggaaa atttggcgaa
    18541 tggaataatg cagttattgg tgaaccccta aaaccattcc gcagggctgc gaatgcaatt
    18601 gaggcttctg gactggaata tacaattctt cgccctgcct ggctgactga tgaagatatt
    18661 attgattatg agcttacctc acgcaatgaa ccattcaaag gtacgatcgt atcacgtaag
    18721 agtgtcgcag cattaatcac agatgtcatc gataaacctg agaaacacat tggtgagaat
    18781 ataggcatca atcaacccgg gactgatggt gataaacaac tgatcaatca gatgcgatta
    18841 acatgtgtag aaccagtaag aataaaatac gccatgtata taacattacg acaattctaa
    18901 tatctgcaca tcaatgaaaa tacaaacaac ttccggcaaa aaagtgatac aagatcgatg
    18961 acatagcgtt gaagaaaagg atttgataat ccgcaaaatt tgcacaacac tgcctgtaat
    19021 acttcctgaa ccagagacga tcatgaccgt aactcgccca cgcgccgaac gcggcgcatt
    19081 tccgcccgga acagaacatt acggacgttc attattgggc gcgccgttaa tctggtttcc
    19141 gacccctgca accagtcacg aaagtggttt gattctggct ggcactcacg gtgatgaaaa
    19201 ctcttcgatc gtcacgctct cctgtgctct gcggactttg acaccttctt tgcgccgcca
    19261 tcatgtggtg ctgtgtgtga atcctgacgg ctgccagtta gggttacggg ccaatgctaa
    19321 tggtgtggat ttaaaccgaa actttccggc agcgaactgg aaggaaggtg aaacagttta
    19381 tcgctggaac agcgccgctg aagaacgtga tgtcgtttta ctgacaggtg acaaacctgg
    19441 ctccgaacct gaaacccagg cgctgtgcca gcttatacac cgcattcaac ctgcctgggt
    19501 ggtctcattc cacgatccgc tggcctgtat tgaagatccc agacacagcg aattaggtga
    19561 atggctggcc caggcgtttg aattgcctct ggtaaccagc gtcggttatg aaacgcctgg
    19621 ctctttcggc agttggtgtg cagatctcaa cttgcattgc atcaccgcag agtttccacc
    19681 catctcctcc gacgaagcca gcgaaaaata tctctttgcg atggcaaact tgctgcgctg
    19741 gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg ccggttccac atctaccgcc
    19801 agccaggtcg gtccgtcaag atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg
    19861 ctaatggcac gagaggtaca caacatgcag cccagcatca gacgaaaacc ttgtgcacgc
    19921 gcttcagtcg ccagcgccag cgcttccgtc agaccaccgg ttttatcaag cttaatgtta
    19981 accatctcat agcgcccttt cagcgccttc agattgctac gagtatgaca actttcatca
    20041 gcacaaatcg gtaacggatg aataaaattc tccagcgccg catcgtcctg cgcaggaagc
    20101 ggttgttcaa gcatcgcaac gcctaaatct gccaatagct ggcaacgcgc cgccaaccct
    20161 tctgcacgcc aggattcatt tgcatcaacg atcagcgtcg catcgggcac agctgtgcga
    20221 attgccacca tccgctcact gataagatgg ttatccagct tcactttcag taatttcgcg
    20281 cctgcctgcc agagtgttga tgcactattg gccatctgat caggcgtacc gatgactatc
    20341 gtctgtgccg tggtgactgt ctcgggaagc gttatgccga tcaaatcagc ctgcgattgc
    20401 tgctgttttc gcgccgccag atcccacaat gcacaatcca gcgcattacg tgctgcgccg
    20461 gcagggagga ttttttgcaa ctcctgccgt gtcagccctt tctctaattg cggcacgacg
    20521 ctcataattt gcgccattac cgaggcatca ctttccccat aacgcggata cggcgtgcat
    20581 tcgccggtgc ctttaatacc ctcttcttcc agttcaacca ccaccacgcg cgcttcactg
    20641 cgacttcccc gagcaatcac aaacggggta tgtaagggcc aggcctcctc aaatacctta
    20701 acggttctca tctttactcc ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt
    20761 ttgctgtgtt attgacagtt agcataaact aggtgtgacg ttaactatat gtaaacagga
    20821 aagataatca tgtcacaaac cgttcatttc cagggcaacc cggttacagt cgccaattcc
    20881 atcccgcagg cgggtagcaa agcgcagact tttactctcg tggcaaaaga tctgtctgac
    20941 gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga acattttccc gagtattgat
    21001 accggtgttt gcgccgcatc agtacgtaag tttaaccaac tggcaaccga gatcgacaac
    21061 accgttgtgc tgtgtatctc tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca
    21121 gaaggtgtga acaacgttat caccctctcc actttccgta acgctgaatt cctgcaagcc
    21181 tacggtgtgg caattgctga tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt
    21241 attgatgaaa atgacaatgt gattttcagc cagctggtgg atgaaatcac caccgagccg
    21301 gattacgaag cagctctggc tgtactgaaa gcataagtta atatgcctga tggtgttgca
    21361 ccatcaggca tattcacgct tactcttcgt tcttcttctg actcagacca tattcccgca
    21421 acttattggc aatcgcggta tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg
    21481 gataattgcg ataaagctgg gttaataccg agcgttcaaa acggctggtg atttcgtcca
    21541 gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca
    21601 aaatatcctg tggacgcagc tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt
    21661 tctttaactg ccgcacattt cccggccacg cataacgcgt aagtacagta ttcagatcag
    21721 cggccagttt cggacgcggc acgccctgct cgtcggcaaa gcgggcgacg aacagctcgg
    21781 ttaacggcat gatgtcctgc ggacagtcac gtagcggcgg cagattgagc gtcaacacgt
    21841 tcagacgata atagagatct tcacggaaca cgcctttttg caccagttcg accagattct
    21901 tctgcgtagc acaaatcacc cgcacatcga catgcacctc atggtcttcg ccaacccgac
    21961 ggaaggtgcc atcattaagg aaacgcagta atttcgcctg catccgtggt gacatctcac
    22021 ctatttcatc caacagcacc gaaccaccgt tcgcctgctc aaagaaccct ttcttccctt
    22081 ccggagcatg accaaacagt tcactctcga ccgcatcttc cggtatagac gcacagttca
    22141 gcgccaggta aggtttgctc gctctggggc ttgcctgatg acaggcatac gcaaagagat
    22201 cttttccggt gccggtatca cccgtaatca gcagcggcgc gcttagcatc gccagtttct
    22261 gcgcctgttc gacaacatgc ttcattttcg ggctgacagc gacaatttga ctgaaggcgc
    22321 tgacatcctg ggcggcgaca ttttgcaact gacggcccat acgaatcgtt gatcgcagca
    22381 tcaccaccgc accggtcagg acgtgttgat cattttcatc ctgaagataa acaggcgtaa
    22441 tctccatcag gaaattctgc ccattaataa cgacatgctc gttatgcgaa tcttgcggtt
    22501 cgctttccag ccagcgtaaa aaattaaagc cgttaatcaa ttgcgcggcg gtatggttgc
    22561 gcaggcgatc caatttttgc ccaaaaagct gacagctcgc cgggttcgcc atatccactt
    22621 tgcttttcat atcgacagag agcacaggtt caggcaacgc ctccagtaac gcgctcaacg
    22681 ccagatgctc acgttcggaa ggcatccacg ggacagtacg cacatcggta acacctgcaa
    22741 tacggcgtat ttcggccatc agactgctga aactctcaaa ctccagttca gcaaaattga
    22801 ggtagattcg cccaatggga tcaatctcaa taccgcgtaa atcaatgcct cttagcacga
    22861 gtagatcgag taattcgcgg gtcagaccga gtcggtcttc acaaaagact tccagacgca
    22921 tgggaacctt cacctgaaaa aagaacaatt aatatgatga tatgacacta tttgaatagc
    22981 aggaaggttt ctgtcaacaa tcattgacac aaagacggaa aaacgggcac ttcggcgtaa
    23041 agatatccgg tcagcacccg atattgcatt atttttcgct gggcgttttg cctttttgca
    23101 gagtttcttt cacctgaccg ataagctgac gacggaaatc cccgaggcga ggtttgtcat
    23161 cgtcaatcca cggcagcggg cggcaaagct ccatagcttt aatccccagt cgtgccgtca
    23221 gaagtccagc accaatcccc tgagctgcgc gggtagacaa acgcgcagcg agatcttgcg
    23281 acatccagtc catccccact tcgcgcacca gttcgctggc tccggcgaaa gcgatattca
    23341 gcaataccag cttaaacagg cgcaaacggc tgtaatatcc cagttcaatg ccatacagcg
    23401 tggcgatgcg attaatcaaa cgcagattgc gccaggcgat aaacgccata tccaccagcg
    23461 ccagtgggct aacggcaatc attaacgttg attccgccgc tgagcggctg atttcgcgcc
    23521 gcgcctgggc atctaaaact ggctggacca agtgagcata caggctgacc acttcacgat
    23581 cgttctgcgt ttcatggatt gaggcatacc agcgctgcag cgccggatgt gactgatcaa
    23641 tacccgcctg ctgcgccagt ttttcgcaga atgcacggcc tttacccgtg ccgtgactgt
    23701 gcaacaaatc gcgcgcttcg tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc
    23761 gccactctgt taccacagaa cccacgccag cgccgataat caatgcccca gcggcacatc
    23821 cacccagcgc cacccagtcc tgggtttgcc aggcgttcat tgtccactgc acgccctgcc
    23881 cgacaacgct tgcgccaaac agagccagcc cgcccatcac cattttgcgc cacaggctgc
    23941 gtttcggacg taacgctgca tccattaccg cttcaacttg cccctcttct tcctgcgctt
    24001 catcgagcgt ggctggggca aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc
    24061 tgaattttgg attctgatcg acctccagtg gaccgtcgaa atcaatacgt ggttttaacg
    24121 gttcggtcat cgcaatttat ctcctattaa aaattccagc gcagcatcaa gacgaatatg
    24181 cggtagcggt ttgtcgacat ccatcacctg cggacgaaac gcctcaaact ggaagccttg
    24241 cttatcccag aacgcctgac ctggcaaacg tgcgggaact tcgccaggat aaacagtgag
    24301 cggtgcgcca tcgctgagtc gattaccgcg cagcgccggg attttctcac cgttaacatc
    24361 aataattccg ctggtggtcg cctgaacaga cgccagcccc agacagtcca tactgatccc
    24421 ttcgaacgcc gcattttgcc aggcatcctg aatcagttgt tgcagcaatg aaaccatatt
    24481 ggcgtgctga tcgatggtca catggtccgc tttagtggca gcaaacaata gcttatcgat
    24541 aaccggcgaa aacaaacgcc ggaacagggt acgctgcccg tagtggaaac tttgcatcag
    24601 ctgcgtcagc gccagacgca tatcattaaa tgcctgtggt ccactgttga gcggttgcag
    24661 gcaatccacc aacacaatct ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt
    24721 caccactttc tcgcagtaat aattaaaccg ctcgcgcaac attccggcat tggtgtgctt
    24781 atcggcctgc gccagtttgg actcgcccca ggcatcgaca tctggccatg ggaagaattg
    24841 cagcgcgggc gctcctgcca tatcgccagg caaaacaaag cgtcctggct gaataaagtg
    24901 cagcccctgc tgtttgcagt gatggagata atcggtccac gcggcggcaa tgtctgccag
    24961 ccggttttcg tcggcaggcg ctagcgggtc cagcccttcg cacatcattc gccatttcgc
    25021 cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc tggcgcgacc agcttaaata
    25081 gtcctgcgcc agcatcggca ggtcgagcaa ccattcgccg ggataatcca caatctcaag
    25141 atacagcgtg gaggtgtcct taaagtggcg cagcagcgaa tcgttcgatt taaaacgtaa
    25201 cgccaggcga atttcactga cgccgcgcgt aggcgtcggc caggcgggag gatcgccata
    25261 taactgcgcc aacccttcat cataggtgaa gcgcggaatg ccaaagtcac gctggggaat
    25321 gcgttttacg cccagcaggc gctcttcacg caccgcactt aaaagcggca aacgtgctcc
    25381 ggcatgaata ttgagcaact gattaaccat cgcggtgata aacgctgttt tgccgctgcg
    25441 gctaagtccg gttacagcga ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt
    25501 gagttcattt ttaagtcgct tcattgctgt cctgtgctgc tctgaagacc agaaaaaaaa
    25561 atcgttcgta aggatatgaa tgtgggggcc aggccattga ttatcaacta cccgtgttgt
    25621 catcgacggg cgaactccat aagatgtgca gcattcacgt tcggacggag ttcctgacct
    25681 atgtccccta ctatttatga tattgccagg gttgcaggcg tatcaaaatc caccgtatca
    25741 cgcgtgctga ataagcaaac caatatctcc ccggaagcgc gcgaaaaagt gttacgggcc
    25801 attgaagaat tacagtatca accaaacaag ctggcccgcg cgctgacctc ttcgggtttt
    25861 gatgccatta tggtgatttc tacccgttcg accaaaacta cggcgggtaa tccgtttttc
    25921 tctgaagttt tgcatgccat caccgccaaa gcggaagaag aaggtttcga cgtgatatta
    25981 caaacgtcgc acaatccggc agaagatctg caaaaatgcg aaagcaaaat taagcagaaa
    26041 atgattaaag gcattattat gctaagttcg cctgctgatg aatccttctt tgcacaactc
    26101 gacaaatacg agatcccggt ggtggtgatc ggtaaagttg aaggtcaata cagccatgtt
    26161 tattccgtcg ataccgataa ttatggcgac agtattgcac taaccgatgc gttaattgaa
    26221 agcgggcata agaatattgc atgcctgcat gcgccgcttg atgttcatgt ttcagtggat
    26281 cgggtaaatg gttataggca aagcctggct atgcataata ttgcagtgcg tgatgaatgg
    26341 attgttgacg gcggttatac ccatgagaca gcattaaaag cagcacggga attattaagc
    26401 cagtcaccgt tgcctgaggc agtgtttgct actgacagcc tgaaattaat gagtatttat
    26461 cgtgcggcgg cagagaaaaa cattgctatt ccgcagcagt tagcggtggt gggttatagt
    26521 aatgaaacgc tgtcgtttat attaacgcct gcaccaggcg gcatcgatgt tccgacgcag
    26581 gagttaggtc aacaaagctg cgagttatta ttccagctaa ttgccggaaa accgtcacca
    26641 caaaatatta ccgttgctac gcatatgtcg ttgaaataaa tatatgccgg atttggcgct
    26701 cgccccgcat ccggcaattt agcacattat cagaacgagt aattcacccc aacacccgca
    26761 taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc gtattccagc
    26821 gaaacggaaa gaccattctg gaaatcataa ccgtaaaata aacccacacg gttaaaatca
    26881 tggccttcac gttcaatatc atcctgccag tcccagttac tccagcgatc cagcccaatg
    26941 cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc gcgaatttct
    27001 tgcgtggaaa actcaccgtt attgcggcta tcatccatat tgaaaccacg ctcgagataa
    27061 tagttcactc gcaaggcaag cgtttcgttg aaggtatatt gcagacctgt ttccgtttcg
    27121 acacgggtat cagcataacc ggtcgtattc agatcgttgg caaatttata catcgacagc
    27181 caaccgttga aacgtaaatc gtcagtcagt ttcacatccc agtctggcgc aattttccag
    27241 cgctgcatat tcgccgtgtc tttacccggt tcatcaacgt agtgataacc ataattacgg
    27301 aaaccaccag tcagaccgaa actgaaatca tcgctttcga ggaactgata atgtacctcc
    27361 agctccgggc gatcaaacca cgttccacgt ttacccgcgc tataatctac cggcccttcc
    27421 tgataatagg ccagagaaat tctccacggc ccgttggcgg cattaaaata gacagaaggc
    27481 tccgccagcc catccatatc ttcgccataa ccctcgacgt tttctatttc gtacatcgcg
    27541 ccgatattaa agtgccagtc gttcttttcc tcggcctgtg cgcaggccat tcccgcacac
    27601 atcaccagtg cggtacaggg taataacttt ttcattattg ttccttgtat tatcctggga
    27661 gaatgccttc cccctgtatt tagcaaattg ctatttccgt ttctgcatca aagaaatgac
    27721 atttagtcat atcaaaatga atagtgatat tttctcctgc atgataatca tttaacgcac
    27781 cagcacggac cactaactcg tgccccccaa ccgtggtgta gagcataaat tccgcaccgg
    27841 ttaattccgc cacgctaatt ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa
    27901 tatcttccgg gcgtattccc attacgatgg gcttatgcaa actttcctgt gttttcagaa
    27961 ccgctaattt ctcttcgggt atggttaatt taagcgtttc cgtaacgaat ttatcgccat
    28021 cgatcgtgcc gcgaataaaa ttcatcgctg gtgatccaat aaatccggca acaaacatat
    28081 tcgcaggttg gttataaacc gttttcggcg caccgacctg ctgaacaatc ccatccttca
    28141 taatgacaat ccgcgtcgcc atggtcatcg cttcggtctg atcgtgggtc acgtagatca
    28201 tggtggtgtt cagtttctga tgcagcttgc tgatctctgc gcgcatttgc acgcgcagct
    28261 tggcatccag gttagagagc ggctcatcca ttaaaaacac gcccgcttcg cgcacaatcg
    28321 cccgcccaag cgccactcgc tgacgttgcc cgccggaaag cgcgcccggc ttgcgtttca
    28381 ggtactcacg caggccgaga atttgcgccg cccagttcac ccgctcatca atcacctctc
    28441 tggcgatttt ttgcatcttc aggccaaacg ccatgttgtc gtagaccgtc atatgcggat
    28501 acagcgcgta gttctggaac accatcgcga tattgcgtgc tttggctgga acgtcattca
    28561 ttcgtttgcc gtcgatcaac agatcaccgc cgctgatctc ctcaagcccg gcaatcatac
    28621 gcagggtggt cgatttaccg cagcccgacg gaccgacaaa cacgatgaac tctttatcgg
    28681 caatctccag gttgaagtcc ttcaccacat gcacctggtt atcgtagatc ttttgaatat
    28741 gttgtaacga aagctgtgcc atgttgattc ctttgctata cgttttgcca gaaggccgat
    28801 aaccgcggcc aggtcaggga gtccgtcgag ggcaacaata attgcgcccc ggttaagccc
    28861 gcgccgatcc ccaccgagcg cataccgctg gcgttaatgg cttcaatgcc cgcctgcgca
    28921 tcttcaatgc cgatacatgc ctgtggcggc acgcccagcc ctgcacaggc ggcgagaaag
    28981 atttccgggt ccggtttcga atttttgagt tgggaagcgt ccgcgcagaa ggtgaaaaac
    29041 tcgcgcagct ccagcgctgc taaaatcgtc ggcgcattca gggagacaga agccagccca
    29101 accggaatct gctgtgctcg gagatctacc agcaaattgt gaatgccagg cagcacagca
    29161 ttaaccgtca gttcgcgcag tgagtggacg taaagcagat ttttgcgata cgccagttgc
    29221 gcccgttcct gtgggttaaa atcgccctct ttacccccgt gctgcagaat tcgccgcagg
    29281 gactcatcgc ggctgatccc ttttaggaat tcgttaaact gcgcatcaat gctgatgcca
    29341 atttcagcgg caatctgctg ccacgcctgg aaatgcagat gcgcggtatc ggtgattact
    29401 ccatccagat cgaaaattac cccttgcagt ttcattcccc ctcctgatgc gtggtagcgg
    29461 tcccattgaa gggcaaaata aaattcccca aacagaaaac agattcttca gcgacgtaaa
    29521 taagctgacc gttcaagcgc agtgaaacgg gcgcagaagt tcgaatcgca atacgctgca
    29581 cgtcgagagt gacctgtaat tcgcagccct gccagaacag agggaaagac aactgttgcc
    29641 actgctcagg taacgccgga ttgagatgta attcaccgtc acgcacgctc accccggcaa
    29701 aaccctgaat cgcccccagc cagatagcgc cagttgcggc agcgtggata ccgtcatcac
    29761 agctatgtgg atcagcacca agatcgattt cagtcccctc gcgccagaac tgataacttt
    29821 gggtcagcag gccacagcgt gcggcaacaa tgccgtggat tgctttactt aatgacgagt
    29881 cgtgaatagt gcgcggttca taaaattgca gattggcaag acacgatgcc gctgagaact
    29941 gctccggcag catgtaattg agcatcacca catcagcttg tttgaggatc tgcatctcgt
    30001 tcacttctgc gcgtgaataa tccagcagaa tggtttgctt ccccgccgcc gctttgtatt
    30061 tcgccagatt aatcaccggc ttagccataa acgaatcatc ctgcggcaaa acgccgtcgg
    30121 gctgaatttc tggcagcagc agttctttga ggtacatttc tgcacgatga ataaacgcgt
    30181 cgtcgctaca gccaaactga cgagcgatat tcagcgcctg ctggacgttg taatacgcca
    30241 tatagctggt gaaggcgtta ttattgacat gttcggtata ttcatctggc ccaataacat
    30301 catgaatttc cagacggtcg ttaaccctca ccgcgcggct aatccagaac tttgccgtct
    30361 ccagaagtag cgccatgcct tcatgagcaa tgaaactttc atcccccgtg gtccgccagt
    30421 attgaataac cgcccaggcg atatcggcca ccagatgatg ttccgcctgc gcagaggcca
    30481 ctttttgccg caacccggta cgaatgttaa tggcagcaaa ttccggcgtc tcttcttcgc
    30541 cgctacgcgc gctttcccac ggaaacagag cgccctgcca gccgttgcgt cgcgctttct
    30601 cctgcgcgcc aagcaagttg tgccagcgat aacgcagtaa acttcgggca accgtcggat
    30661 cgctaaacag atggaacggt agcaaaaata cttctgtatc ccagaaaacg tggcctttgt
    30721 agccttcgcc ggttaaacct ttcgccgcaa tgctgctgcg ctcatcgtgg gcaggcgtca
    30781 tgatgcgcag gtgataaagc gcataatcta acgcttgctg atcgtgcgca tcgccgccgt
    30841 ttaccgtgat acgacgtttc tgccaccatt gacgccagtt ttctgttgat gctgcaagaa
    30901 gttggtcgta actctgctgc gcgcacattt caagctgacg aagcgatgca ctaccccact
    30961 cgtctaaagc gacgtgtctg tcgtcccgcc agtcgatcca cacccgtttt tgcagcgtca
    31021 gcgttttgcc cgcaggtagt tgtgcactgg tatgttgcag caagcggcgc tctttggcgg
    31081 tatagcattg ctgcacgtca ccgctcacct ggcagcaaca gctgatggcc acatcactgc
    31141 gtccatcctg agtggtatag atcccctgca tcagatgctg accaaatacc cgcacctggg
    31201 tttcgtcgag atgttgtcgt ccgtggttgg tttgcgtggc gtcaatgcct gttgaaatca
    31261 gcactgacgc gtcagcgtcc agcggcgtaa tgctgatttc cagcgccatc agcggcagtt
    31321 gttctgccga aacaaagcga cggctggcaa tggcataacg tgcgccgctg gctgaagacc
    31381 agacgacatt gcgacgtaat tcgccactgg caaagttaag ctcacgctgc catgcttcgc
    31441 gggataacga gataatctca ccatttatgg caatctccat ccccacgaca tcaggcaggt
    31501 tcaccagttc gttgatttca cccttacccg cccgatgata cagccccgtc agatacatgc
    31561 cgcgcgtctg gcgcgtgtaa tcttcttcat ggctggcgcg aagcccaaga tagccgttcc
    31621 cctgcgccat cagtgaagca tatttgttca gtgtatgctg gctgaaatgg ggttctgata
    31681 acgttactgg cctggtcata attccacacg cgtccctgtt tctgcggatt gatacaacgc
    31741 cgccaccagt tgctggatga tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc
    31801 ctgtacgtga ttaataaagg cttccatgct gcgcaaatgg cggttgtcgt ctgccatttc
    31861 ccgttgcatc agcgtcatta attcaccgtt gttatcggtg tagatatgtg ctggaaacag
    31921 cgtcgcaccg gctttatcac cacaaaagct gacgttcata atcgactgtt cgcggatgtt
    31981 gagtgcaaat gacgtttcca gccacagaat gccgccgtta tgaaattcaa tggtgccaaa
    32041 cagcgaatct tcgacgctat aagttgccgg atcccactcg ccaaattgac cacagctctt
    32101 ttgcgtgccg atcttttgaa aactatgcgc attcacgctt ttcaccgccg gaaagcccag
    32161 cacatacatc gccgcatcca gcatatgaat gccgatgtcg atcagcgggc caccaccctg
    32221 cagttcttta ttggtaaaga cactccagcc gggaacgccg cagcgacgca gggcgcgggc
    32281 ggtggtaacg taaatctctc ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt
    32341 atcgagcgca aaacgatggt gaaagtcgta ggccagcacc ttgcccagtt tgcgcgcggt
    32401 atcgcacatt tcccgcgcct gttctggcgt catggcgggc ggtttttcgc acatcacatg
    32461 gcagcccgct tccagtgcca tcagggtatg ttcgtaatga aaacggttag gtgagcagac
    32521 gctaaccaca tcaggtttca ccgccagcag catggcctgc ggatcgtccc acacggatgc
    32581 attcccgtat ttttctgcca gcgcctgcgc ctgggaaagg cggctgtcac agacagccac
    32641 cagttccaga tcgttgcggg tgcagtagta cgaagcatga actttatccg ccacctggcc
    32701 cgcgcctatg atcgcgactc gcagcggaga gcttgtcatt gcacttttca caaagacctc
    32761 ttagcaggta cgcacccagg ccaacgaatc acggtacgcc tgggcaggat cttctgcccg
    32821 gatacgccct tcatacacca cataaccctg atagttatcg gcgcgcagct gttcaaacag
    32881 cgcgtggaaa tcgagggtgc cgctgcccgg ctggtaacga tggttgtcgg caatatgcac
    32941 atgaccgagc aagtcgcggt tgtcgtgcaa cgcctgcgcc aggttatctt cttcgatatt
    33001 catgtgatag aaatcgccga taatctgcac atgtttaaga ttgttttcga cgatgtaacg
    33061 acgagcatcg gcgagggtgt tgatcatgtg atcctgatag cggtttaacg gttcgaggta
    33121 caccacggtt ccggtacgtg cggccgcctg ttccagtacg cgcagggaat cactcaccat
    33181 tttgcggtcg ccgtccaggc tacgcggcga ggtcatcggc ggtaagcgga aggtaaacat
    33241 gccccatgca gccggaacga cgataccttt accgcccact tctgccagag cttcaagaat
    33301 gcgttcgatc tgctttacgc cattaagtcg acgctcttcg ataaagtcgc caatccaacc
    33361 gtcatagcca ccgcaggcgg tggtcaccgg taaaccggtt tctttgattg ctgctttgac
    33421 ttcttcgaga ttgttgacca gcagtttgcc gtcgatctca aaaccatcga aacccatttc
    33481 tttgatataa cgaaatttct ccagaatgtt ttccggaaag aacgcctgat tctgtgtgcc
    33541 gattttcatt tttttcattc ctttgaatta aaacgtaacg cccattttga tgctctgttc
    33601 cggatgctgg tcgacatact gcatgtaact ttccgggcta ttggcaaagg tcaccaccgg
    33661 gtcgattaaa tcttcacaat tgagataacc gttcatcagc agttcccaac aggtttcttc
    33721 gatacgtttg cggctccagc gcggataatc cgggttcggc tcgctgcacg cacgggagaa
    33781 aacaattttg gcgttattga aatgggcttc gcgtccgagg ttaaaacctt cggcaaacgg
    33841 tttggcaaac gcgacatacg agatggtgcc gccatatgcc aggccgcgaa gcgccgattg
    33901 cagcgcgtcg gcgtaaccgc tggtttcgat aatcacatcg gcaccctgct tgccggtcag
    33961 cgttttgatc tctttgccta cgtcagtgcc aatagggtta aggcagaaat ccgcaccgtg
    34021 tcgacgagca atatcacaac gatgggcaat aggatcgacg ccaattacca ccgatgcgcc
    34081 agcgcgtttc gccagttgga tggcaatttg accgatcgcg ccaagcccta ccaccacgac
    34141 aaaatcccct acgcgtacgt tggcatcgcg cacgccgctc atggcaaact gcgccgggtc
    34201 gtaacagacg gcgtttttcc aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt
    34261 cactgcgtta atgatgaccg tctcggagag cgggccgtag ccgcatacgc tgtcgcccac
    34321 cgcgtagtcg gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag
    34381 ctggaatttg ccaaactcaa taccgcgcgg cgcatctgcc ggacgcgggg taaacatctg
    34441 ccattcgccg ttaaagtctt catcgataaa cgggctggcg gcgcggaaat cgaccacttc
    34501 cgttccatgt ttcggcgcgc cgaaacgcac gcggatcttc acttcactgg ctaaaatcgc
    34561 ccgatcttca tactcaacca gcgcagctac gcgcggtgct gtggctacta actttttcat
    34621 gtaaaactcc ttataaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc
    34681 actcttaata aaacgctccg aaagcgcata cataatcacc accggcaatg cggtcaccag
    34741 cgaggccgcc atcatccgtc cccagatgta atcaggcgta ctgaacagcg cgttcaggcc
    34801 gactggtaag gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt
    34861 ccatgccacc ataaagcagt agacgaatac ggatatcagc ccggacatcg ccaggggcac
    34921 ggtgatacgg aagatgattt gcagacggtt gaggccatcc atcatcgccg cttcttcgat
    34981 ctcatcaggg atggtgtcga agtagctttt cagcataaac acggcggtgg gcagggtttg
    35041 cgttaccatg gtgatgatca gcgccatctc ggtgtcgtat atgccaagcg cggtgatgat
    35101 tttgaacagc ggcaccacca gcaaaatgcc ggagaacata tacaccgtgt aaaagctggc
    35161 gttgatggtc atccgccctt taaagcgcag gcgggaaagc gcgtaagccc cgagaatgcc
    35221 gagaaatacc gccaccacgg atgaaaccac agacaccacc agactgttac ggaagtagtc
    35281 gacaaacggg aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg tccactgctg
    35341 tggcagcagc gtaggatgca gcgagatcgc ctcttttgcg ctcttgaacg aggtcatcag
    35401 catcacaaaa aacggaaaca gcatgatgat gagaaacagc gccagcccgc agtaaaaacc
    35461 gatgcgactg agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc ctggtcagca
    35521 gaatgacagc gaaaataatg atgaagagca ccaccgagat cgccgccgct ttccctaggt
    35581 cattaaaggc aaaggcggtt ttgtagagat agacaccgag aatatcgacc ttggtcgtca
    35641 gcagataaac atccgcgaac atgtagaaca tccagatggt gcgcagggtc accaccgtcg
    35701 ccaggaccgg cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag gcattagcgc
    35761 catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg
    35821 aaataaaggc ataggggaag tagcgccaga tggcgaacag caccaccagc acaaagctac
    35881 tgcccggatt atcgaaccac agcggtgcct gctcatagag atgcagaaga tcgacgccga
    35941 ggtagttcac aatgccgtaa ccgttgttga acatgtattt ccaggcgaac accagcgaaa
    36001 tagacggcgt tacgtaggag aggagcacca gcgaacgcgc ggttttgcgc aggcggaact
    36061 cgcggttgaa aaacatcgcc acggccagcc cgagcacggt gctgcccgcc accaccagtg
    36121 tggtatacca gaccgtcatc cacagcgagt gccagaagcc gggatcggag agaatacgca
    36181 cgtagttgct taccccaaca aacgttgact cgatgttggg attgagcggc agacgtaaaa
    36241 aactgatttc gatattcgac accattggcc acgccaccag accgcccagc agtaataagc
    36301 tgggcgcgag aagcagcagc gcaaagggca tatcggaacg acctgaaaaa agcctgttca
    36361 ttgcggattc ctgtcctggg tttagcgctg ttcaaccaac tcatccagct ttttctggct
    36421 cgtttgcagc gtggtagaga ggtcggcttt acccacggtg acgttatgca ccatggagct
    36481 caccacgcca gaacccgtca catcgcccat gcgggtaaaa tttttatccc ctactgcgcc
    36541 aaagacctga atatttggca gttcgctgat taactgattt ggcaattctc ccagcgcctt
    36601 aataacgtcg ttgtctttcc aggtggcggt agtgacaacc gctttgttca ccggcagcgc
    36661 cgcgcctggc gacatcatca cccagtcggc aatgttgtct gcctgctcca taaaggtgac
    36721 aaatttttca gccgcttcgg tctcttcgct cttttgcccg gcggtaatgg tcagcgaggt
    36781 caacatgccg tagaccgcag agtttttctc ggtgggtaca acgaaaccaa cgtttttcgg
    36841 atcgccctct ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt
    36901 gccgttcata aaagcgtctt tcacttccat gatgtcgtta gaacctggca tggtgttggc
    36961 agcaaggttg cggtaatagg tcagcgcctg catcatctct ggtgtatcaa gggtgatttt
    37021 gccttcggcg ttaaagacgt tagcctgatt ggataacgca aactgggaga aagactgttc
    37081 cgtcaacacg ctttctgctg taggcagcgc aataccgtat tttttattcg ccgggtcatt
    37141 cagtttctgt gcaacgtcca gcaattgctg ccagtttttt ggctcctcca gcccagcttt
    37201 tgccagcaca tctttgcgat accagatccc gccaatccat gcgctgacag gaacaccggt
    37261 ccaggcgctg ccatcttcgg tgcgcacaat ccgcagtacg ccatcgtaga acgcgccttc
    37321 accgacgttg ctgatgaccg tggcaaccgc ctggcgatcg ataagctgct ctttgtccat
    37381 cactttggcg tagtcatggc tggtttcgat cacttccggc agcgaaccgc tacgtgaaag
    37441 ggtaatgact ttggtgttat aggcgtcttc ttccacgggc acttgtttta cggtgatacc
    37501 tgggttttct ttttcaaaac gggcaatcaa tttgctgatg acggcctggc gctcctgctc
    37561 caccgaagaa tgcataaatt cgatggatac attattttta ttttcttctt tacagcctga
    37621 aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg
    37681 ttccttttta tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccacta
    37741 tatcaacttc actaatcaga tcccgaccgt gcaggtcagt aatatttata tgctgaatat
    37801 tttcactgac attaaacaat cctgtcagac aattaccatc cgcgttgctt ctttgaatac
    37861 acattacgga tgaattaacc gtgtcgatgg taaaatcatt atccggatga aactcgttat
    37921 gactgcaacg aagtgtaatt aaacgcgaca acttataata tatcgaatac cttaatgtcg
    37981 ctttattgtt cagttctgtc gttatctctt tactagagta ttttttacgg ttaatcgcac
    38041 gattatatcc aagtttttcg acacctgcgt aatcattacg cgaccccaga atgctttgaa
    38101 tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg
    38161 cacaccgttc ttcatcgcta ctctcgcggc ggcttaacgc atccatatag gtgacattta
    38221 tttcatacgg gctacgcgta ccgtcgggat tatttttcca gtttactaat gcaccttcct
    38281 gctgtaatgc ctcgaccagc gctaatattt cgctttctgg taacaagcca cgcagtgggt
    38341 tcagcccgat gccatcgtga gaggcgagga agttaaacca ggtggtgttg ctggaaggta
    38401 gtgtcaggtt ttgcgcccac gcacaaagcg cctcaacgtt ctgtttttgc accgcatgca
    38461 gcaccagcgg cggcagcgag aactggtaca ccatatgtgc ttcgtcatcg ccttcgccaa
    38521 agtaagcaat gttgtcttta tgcggaacat tggtctcggt aatgatcact gtgcctggcg
    38581 cgacgttatc aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga
    38641 tgcagcttgt tcccggctct ttccacataa agccaacggc atccagacgg acgtattccg
    38701 cgcccttttc caggtaacac agcaatatat ccaccatcgc cagcaacact tcagggctac
    38761 ggtaattgag gtcaatttga tcgtcgctaa aggtggtcca taaatggcgc gtggaattat
    38821 catccatttg aaacggtgtt aatagcggta acgcacgcgg acgggtgacg gcgctgagat
    38881 cggtttgcgg atcaacggca ataaaaaaat cttcaaaacc tggatgctgt tgtaaatagt
    38941 ttttaaacca ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac
    39001 tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat
    39061 aatcaattac cgaaaaccca tcatcagatg accatggata aaaaggtaat aaatgaacat
    39121 gtgaaaaaat gctttgcagc cattgatggt aaaactgatt aaatgtgggt agtggtttta
    39181 aatcattact gtgaaattga tcggcatagg tgattaacac gacatcactt tcatcccagt
    39241 gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct
    39301 gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct
    39361 gtttcatgtt gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcataaaa
    39421 taaactgtca attgagctca aggggtttag tcacgcagga aatctttttt tacctgggaa
    39481 aaagcgtatt tgtgagctaa cgcaaataat ctatggggat gtaaataggg tgtgacagaa
    39541 aaaacggcgc ataagcgccg ctcatggtga attcttttaa cctttgacct tcggcattgc
    39601 gatgtctttc aggccaccgg cgttctcaac gtgggtatat cccatctcgc taaggatctc
    39661 ttttgcctgc cctgactggc gtccggcatt gcaatacact ttcacggtgt cgtttttatc
    39721 cggaacggcg gtggcaatgc gctctttcac ttccttcaag ggaatattga tggccccctg
    39781 aacgtgttct tgctgatact gctctggaac acgaacatcg atccagtgtt cagcggcgaa
    39841 aacgggcagt gaaaacacca gtgtcagagc gagtaagcct tttttaaaca tgtaagcgtc
    39901 cttctatgga tgaaaattgc tatccattat acgcctgaga tttaaccatt tttgcgaaac
    39961 tcccctcacc ttttataacg ctgcgccagt ttattggcag cccgacttaa cagcggttcc
    40021 agcgccacag ccagcaacat tttcagcggt cggcgagcaa ctgattttat cgcccagccc
    40081 gccacacccg ccgggccata gcgcagtgcg gttagaagta ccagcttgcc tgctaatttg
    40141 aacccaggct tcaccttttg cccggcctgt tgccagcgag tattcataac tttcctcaca
    40201 gttgacggaa acggctacgc agcgtgaaag tgtcggaagt gacataacgt tccatctcgc
    40261 gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc atccagcaat tcgctgctgg
    40321 aaggtagctg ctcaccaaag accatgttgt ccggcattgg atcaagcgca aatgacaaaa
    40381 tgatgtaagc aaccagggta aacagcgcca gaccgaagaa aatcgacagc accaccagga
    40441 tacgcaccag ttttaccggt acatcaaaat agttggcaat cccggcgcag acgccacgga
    40501 ccatgccctg ctgcggaata cgccataatt ttttattgag attaatgccc gccattagcg
    40561 atccctccag ttcggatgtt ctgcatcaag aatggattcc agcgcctgaa tacgctcgcg
    40621 catccgtttt gcttcatcag ccagttgcgc taatcgctgc tgctcacttt gcggcaattc
    40681 actgcgaccg gaacgattgc tgtaatgcag ccataaccag atcggtaaaa caaacagcac
    40741 aaaaatggtt aacggaatag ccagaaatag cgcgctcata agtactcctt agacgatgag
    40801 cggcggcgcg ttcggacgcc gcctggatgt tattattgat tgtcttgctt cattttggct
    40861 tttaattgtg ccagttgctc gctgattgca tcatcggctt tcagttcggc aaactgatcg
    40921 tccagcgatt tttgtttacc gaagctgtgg ctttctgctt ccgcttccat ctggtcgata
    40981 cggcgttcga aagattcgaa acgagccatt gcttcatcca gtttgccact gtccagctga
    41041 cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta acatcaatgc ctgctggcga
    41101 gcgcgtgttt cgctcaattt gttttccagc tcgccgatct ctttcttcat acgcactagc
    41161 gtatcgtcca ccagcgtcac ttcatgttcc aggctcttaa tcagatcggt cagtttctgt
    41221 ttttcaatta acgccgcacg cgccagatct tctctatctt tcagcagtgc cagttccgct
    41281 ttttcctgcc attcaatttc acgcgccgac gcttgttcaa tacgacgagt cagctgtttc
    41341 ttttctgcca gcgcacgcgc cgaagtagaa cgtacttcaa ccaatgtatc ttccatctct
    41401 tggatcatca gacgcaccag tttctgtgga tcttccgctt tctctaacag agcgttgatg
    41461 ttggcattca cgatgtcggc aaagcgagaa aaaataccca taatgttgtc ctcttgattt
    41521 ctgcgttcag gattgtcctg ctgaactatt aatacaattt gcgtgccaat tttttatctt
    41581 tttgatttat aaagatctga ttgaagaatc aacagcaaca tgccaggatg aattagcgaa
    41641 ttacactaat aagtggcgaa tttcatcatg gcagaataca aagataattt acttggtgag
    41701 gcaaacagct ttctcgaagt gctggaacag gtttcgcatc tcgcaccgct ggacaaaccg
    41761 gtgctcatca tcggcgagcg cggcacaggt aaagagctga ttgccagccg tctgcattat
    41821 ctctcctccc gttggcaggg gccgtttatt tcccttaact gctcggcgtt aaatgaaaat
    41881 ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt ttaccggtgc gcaaaaacgt
    41941 catcctggga gatttgaacg tgccgatggc ggtacgctat ttcttgatga actcgctacg
    42001 gcaccgatga tggtacagga gaaattattg cgcgtgattg agtacggtga actggagcgc
    42061 gttggcggca gccagccatt gcaggtgaat gtgcggttgg tatgcgcgac gaatgccgat
    42121 ctcccggcga tggtcaatga aggtactttt cgcgctgacc tgctcgaccg gctggctttc
    42181 gatgtagtgc aactgccgcc cctgcgcgag cgcgaaagcg acattatgct gatggcagaa
    42241 cactttgcca ttcagatgtg tcgggaaatc aagctgcctc tgttcccggg gtttacggag
    42301 cgcgccagag aaacattgct gaattatcgc tggccgggaa atattcgtga attgaaaaac
    42361 gtggtggaac gttcagtgta tcgccacggc accagcgatt atccgcttga tgacatcatt
    42421 attgatccct ttaaacgccg tccatctgaa gaagccatcg ccgtttcaga aaacacttcg
    42481 cttccgacgc tgccactgga tttacgcgag tttcagatgc aacaggaaaa agaactgctt
    42541 caggtcagct tggaacaagg gaagtataac cagaagcgcg ccgctgaatt gctggggtta
    42601 acctatcatc agttccgcgc gttgttgaaa aagcaccaga tttagcgaag ttgggcaata
    42661 caacttgccg gatgcagcgt gaacgcttta tccggcatac ataatcaata cgtcgtttgc
    42721 aggcctgata agcgctgcgc atcaggcatt attgcgttaa ccctgcttct gcgtattaat
    42781 ctctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcgcaacc gggatggtat
    42841 gggcttcgtt aggcggcgca tatttgtggt atttgatccg ccgctatcgc aaagtgccgt
    42901 tgtatgatgg cgacagaacg ccggtgaacg aaacgtaata gtagaatgcg tggcgcatta
    42961 tcctgcctga gggtaatgca ccgcgccatt aactataaaa atatgtatga cccggatcac
    43021 gagtgctatc atccgccttt atgcttagcg cattaacccc aggggcaaat aatgaaccac
    43081 gatatcccgc taaaatattt tgatattgcc gatgagtacg cgaccgaatg cgcagagcct
    43141 gtcgcagacg ctgaacgcac accactggcc cactacttcc agttactgct cacccgtttg
    43201 atgaacaatg aagagatcag tgaagaagcc caacatgaaa tggctgctga agcggggatt
    43261 aatcccgtac gcattgatga gatcgcagag tttttgaatc aatggggtaa tgagtaatta
    43321 tttaattgca gactatcggt cagtatcgtt gcccggataa agcgttcgcg ccgcatccgg
    43381 ctaaaaatga cacgcacagc agcgtgcttt aatgggtttc tcacgatcgt accgctctgc
    43441 tcctcgctaa gttgttctgt gagttatagt tataaagaaa tcacgggtac gaatgttgaa
    43501 ctgatggcga cgccacctgt tcaatgacca accacacggc cctcattacg acctctgaac
    43561 acaaacggta atggcaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    43621 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    43681 caggcaatcg ccgcgcctga atcccccccg catgctgata tccgcgacag cggttttgtc
    43741 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    43801 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    43861 cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    43921 ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa
    43981 atgaatgccg atgatgtggt gtttaccttc caacggattt ttgaccgcaa caacccgtgg
    44041 cataacgtca acggcagtaa cttcccctat ttcgacagtc tgcaatttgc cgataacgtg
    44101 aaaagcgtcc gcaagctgga taatcatacc gttgagttcc gtctcgccca gccggatgct
    44161 tcttttttgt ggcacctggc gacccattat gcttcggtca tgtcggcaga atatgcaggg
    44221 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg
    44281 tatcagttgt cggaataccg cgccgggcaa tatattcgct tacaacgtca tgatgatttc
    44341 tggcgtggta agccgttgat gccacaagtg gtggtggatt taggctctgg cggtacagga
    44401 cgattgtcga aactcctgac cggggagtgc gacgttctgg cctggcctgc tgccagtcag
    44461 ctatccattt tgcgtgacga cccacgcttg cgtttaacgc tgcgtcctgg gatgaatgtc
    44521 gcctatctgg cattcaacac cgccaaaccg ccgctaaata atccggcggt ccgccatgcg
    44581 ctggcactgg cgattaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    44641 acggctgcct ctattttacc gcgcgcctcg tgggcctatg acaacgaggc taaaattact
    44701 gaatacaatc cagcaaaatc gcgcgaacag ttgaaggcgt tggggctgga aaacttaacg
    44761 ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc ccagtccact gaaaaccgcc
    44821 gaactgattc aggcggatat ggcacaggtt ggcgtaaaag tggtgattgt gccggtagaa
    44881 ggtcgttttc aggaggcgcg gttgatggat atgagccatg atctgacgtt atccggttgg
    44941 gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt tactgagctg cgcggcaatt
    45001 cattcacaga ccaacctcgc ccactggtgc gatccgaaat tcgacagcgt attgcgtaag
    45061 gcgctctctt cgcagcagct ggcggcgcgt attgaagcct atgacgaagc gcaaagtatt
    45121 ctggcgcagg aattacctat tctgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    45181 tatgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    45241 cgcgagaaac aggatgaggt gaaaaagcca tgattatctt caccttacgc cgtattttgt
    45301 tattgattgt gaccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcaccc
    45361 cacatgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc
    45421 tgatccactg ggattttggt gtttccagca ttaacggtca accgattgcc gaacagttaa
    45481 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    45541 ttggcatccc cgtaggaatg attgccggga ttacgcgcca taagtggcag gataacttga
    45601 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgctga
    45661 cgctgttttg ttcactcaca ctaggttggt tgcccgtttc tgggcgtttc gatttgctct
    45721 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccgt
    45781 ggcgggatga aatgatcatt agcgcaatcc gtcatatggt attgcccgtg atcaccctgt
    45841 cggttgcgcc aacaactgaa gtgatccgac taatgcgtat cagcactatc gaagtgtatg
    45901 accagaatta tgtaaaagcg gcggcgaccc gcggtttgtc acgctttact attttgcgtc
    45961 gccacgttct gcataacgcg ttacctccgg ttattcctcg cctgggctta cagttttcta
    46021 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    46081 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    46141 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    46201 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    46261 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    46321 tacggctgtg cggggctggc tgtactgtgt atttttggcg gctggtttgc gccttacggc
    46381 atcgaccagc aatttctcgg ttatcaatta ttgccgccgt catggtcgcg ctatggcgaa
    46441 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    46501 ggagctgcgc caaccgtagg tggcgcattt gtggtgacgc ttgccgcgac gatctgtggc
    46561 ctggtactgg gcaccttcgc cggggcgacc cacggcctac gctcagcggt gcttaaccat
    46621 attctggata ccttactggc gatcccttcg ctgctgctgg caattatcgt tgtagcgttt
    46681 gccggaccga gtttgtctca cgccatgttt gctgtctggc tggcgctgct gccgcgtatg
    46741 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    46801 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc caacatcacc
    46861 gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg caattctcga tatcgccgcg
    46921 ctgggctttc tcgatctcgg cgcacaactc ccctcacctg aatggggcgc gatgctcggt
    46981 gatgcgctgg aactgattta tgtcgcccca tggactgtca tgctgccagg tgcggcaatt
    47041 atgattagcg tgctgttggt taacctcctt ggcgacggtg tccgtcgtgc aattattgcg
    47101 ggggtggaat aatgccatta ctcgatattc gtaacctgac cattgaattt aaaaccggtg
    47161 atgagtgggt taaagccgtc gaccgcgtaa gtatgacctt aaccgaaggt gaaatccgcg
    47221 gtcttgttgg cgaatccggt tccggcaaaa gtttgattgc taaagcgatt tgtggggtga
    47281 ataaagataa ctggcgtgtt actgctgacc gtatgcgttt tgatgatatc gatttgctgc
    47341 gtctctccgc acgcgaacgg cgcaagctgg ttggtcacaa cgtgtcgatg attttccagg
    47401 aaccacagtc gtgtcttgac ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc
    47461 ctgcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca
    47521 ttgaactgct gcaccgcgtg gggattaaag atcacaaaga tgcgatgcgc agttttccct
    47581 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc cattgcactg gcgaatcaac
    47641 cgcgtctgct aattgctgac gaaccgacca actcaatgga gccaacaacc caggcgcaaa
    47701 tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac cactattttg cttatcagcc
    47761 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    47821 cggtggaaac cgcgccgagt aaggagttgg tgacgatgcc acaccatcct tatacccagg
    47881 cgctgatccg cgcgatacca gacttcggca gcgcgatgcc gcataaaagt cgcctcaata
    47941 cgctgcccgg cgctatcccg ctgctggaac agttaccgat tggttgtcgt ctggggccac
    48001 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    48061 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgcta
    48121 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggctggtt tcgtcgtcag
    48181 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    48241 atcggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagaa
    48301 cctaccagcg gcgagttatt gattgacgat catccgctgc attttggcga ttattccttc
    48361 cgcagtcagc gtattcgcat gatttttcag gatccttcca cctcgttgaa tccccgtcaa
    48421 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccgacctgga accggaacag
    48481 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    48541 tactatccgc atatgttagc acccgggcaa aaacagcgtc tgggtctggc gcgcgcgctg
    48601 atattgcgcc caaaagtcat tattgccgat gaagccctcg cctcactgga tatgtcgatg
    48661 cgttcgcagt tgattaatct gatgctggag ttacaggaaa aacagggcat ttcgtatatt
    48721 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    48781 cagggcgagg ttgtcgagcg aggcagcacc gcagatgtgc tggcatcgcc gctgcatgaa
    48841 ctcaccaaac ggctgatcgc cggtcatttt ggcgaagcat tgacggcgga tgcgtggcgt
    48901 aaggaccgtt agtgttttgc acctcatcct gacaggcccc cctcaggatg aggtgttcat
    48961 tacttttgat tgtcgattct ggtgagagca accagcaatg ccagcgtata acacaccgta
    49021 attgccagac tcatttttaa cagtgtttct ccgccaattc ccgacaatgg cgcggctata
    49081 ccgccgaaga caaacatgct catccccatc aacgcagacg ccgttccaga ttcctgcgtc
    49141 cctactgcac tcatagcctc tgccccgcca acagtgccga tgccgctaca aaatgcgatg
    49201 gtgaaaaata atgccaccag tgccggaatg ggcaattgtg tccatgcgca taagaccgtg
    49261 agcaatgcac acaaaattgc cgctatcagg ccacctcgca acaatgtcat cgcgttaatc
    49321 cgtcgcgcca ggcgcgagaa gatccatgaa gcaatgataa gtccgatgcc gttaaggcca
    49381 aacaccaggc taaattgcat tggactaaag ccaaattcct tctgcaacac gaacgacgaa
    49441 gagccgatat atgcaaacaa accggccagc ataaaacttt gaatcaggca aaagcgcata
    49501 aaacggcggt tctgcaccac gcttcgtccg gttaatagca atgatgagcc ccttttattt
    49561 tctggcaagg tctcattaat aaataacagg cagcccagca acagtacggt gctaatttca
    49621 gccattaccc agaataaagt gcgccagtca aaagtgctaa caatgtaccc gcccagcact
    49681 ggcgacaaca ccggtgccag gccattcacc gtcattaaca gcgcaaaaaa ctgggtcaac
    49741 gttactccct gatatttgtc acgagcaata gaacgagaga gcaccgaacc acccgccccc
    49801 gcgatccctt gaataaaacg ccagaccacc agccagtaaa tattgttcgt tgtcgcgcac
    49861 aaaatggaag aaacaataaa caatagcaac gacaagatta acggacgttt acgccctatt
    49921 ttgtcgctca gagggccaaa taacagttgt ccgacgccaa gaccaatcag tgatgcggtc
    49981 agggttaatt gcgttatggt cgtggttgcc gccagttgct cagacatctc cggcaaagcc
    50041 ggcaaataaa gatcagtaca catcgggccg ataccggcta aaagaccaag aatcaatgcc
    50101 catgaaagag agactctagc catattattc cttacttgag tgatgacatg ccgccgccca
    50161 gcgactgcca gagcgtgatt cggtttgtaa aatcagtcag ttgcagtgcc agcagctcct
    50221 gctgtgcgct ccacaagctg cgctgcgccg tcaacacggt caggtaatca ccaacacccg
    50281 cctgatagcg acgcaagcca acatcgaccg tttgttgttc agctttaaca tactggcgct
    50341 gtgcatccag ttgctcttca agcgtggtcc gccgcgccag tgcgttagcg acatctttaa
    50401 atgcgctctg aacggttttt tcataggtgg caatgagtcc acgcttttgt gcttctgcgt
    50461 aacgaagctg cgccagatta cttccgccag taaacagcgg taatgtgacc gatggcgcaa
    50521 atgaccagat ctgcattccg tggctgaaca acgacgacaa cgcgtcactg ccaacaccgg
    50581 cactggcagt cagcgaaatc gtcgggaaaa agttagcccg cgccgcgcca atatctgcat
    50641 tggcgctctt caaattatgt tccgcttcct ggatatccgg gcgacgcagc agaacatcag
    50701 atgatactcc cgcaggaacc agacttatca tttgttccgg caggctttcc agtgttcccg
    50761 gtagcagatt ttccgcaagc gttgtacctg ccagtaagtt cagcgcattt ttatcctgca
    50821 tcacttgtgt ctgatagctg gcaacgctgg cgcgcgcttg ctgataaaca ctcatcgcct
    50881 cactgacatc tgtcgctgcc gccgtgccaa cctgttgctg gcgctggata attttcaatg
    50941 agttttctgc actggccatc gtttcttttg ccagggccag gttgctgtta tcagccgcca
    51001 gtgtcagcca ggcggtgctg atttcagcaa tcagcgttaa acgagtgttc tgggctgtaa
    51061 attcactggc aagccaggtt tcccgcgccg cccgagataa actctgatta cgaccaaaca
    51121 gatctaacgt ataactggac accgtgccgt cagcctcggc agttgttccc gtaccattcg
    51181 ccagcgaacg actgcgagtc gaacttaagg ccgcattcac cgtcggaaat aacgacgcat
    51241 tagtttgccc aaagagcgcc cgtgctgagt caatatcagc aatcgctttt tgcacatcgc
    51301 ggttactgtt cagtgcaatg gttactacct gctgcaatcg gggatcgtgg atcacctgct
    51361 gccagtcatg gctgattgcc ttcccctggc cctgtgctcc cggcaaggtc gccgggattg
    51421 gggattcagg cgtactgtag tgaggatcga gcgagacaca tcccgcactc aacaacacca
    51481 gaaaaataag agaacgacgc aacataactt actcctgacg gcgcggttta ccggcaaaca
    51541 aacgtttcac cagtacaaaa aacagaggaa cgaagaaaat ggccagtaat gtagcggtca
    51601 gcgtgccgcc aataatgccc gtaccaatgg cgatgcggct gttcgcccct gcgccggttg
    51661 ctatcgccag cggcattacc cccgcaataa acgccagcga ggtcatgatg attgggcgta
    51721 aacgagtctg cgccgcgcgt aatgccgcac gactcaagga atagccctct gcaaccgccg
    51781 cttcagcaaa ttccacaatc agaatggcgt ttttcgacga caagccgata gtggttaata
    51841 gcgccacctg gaagtaaaca tcgttgttta aatcacgcat ccaggccgcc agcgccgcgc
    51901 caagaagccc cagagggatc accagaatga ccgagaacgg gactgaccag ctttcgtaca
    51961 gtgctgcaag gcagaggaaa accaccagaa tagaaactac atacagactt aatgcctgac
    52021 cgctggccag tttctcctgc aatgacaaac cactccaggc ccaggttgtt cccgcaggaa
    52081 gactgtttgc cagttcctcc atttttgtca ttgcatcgcc ggaactaaag ccagtcgcgt
    52141 tttcgccctg aatttcatag gctgccgagc cgttgtagcg caccagtctt tccggtccat
    52201 acagccagcg ggtggtcgca aaagcagaga atggtgtcat ggcgttatcg ctaccgcgca
    52261 caaaccattt acctaagtct gacggcgcgg aacgaaattc gctgtcgccc tgaatgtaga
    52321 cttttttcac acgaccacgg tcaataaagt cattcacata agtaccgccc cacgcactgg
    52381 acagggtgtc ggtgacatca ttcaaactca accctaatga caccgcttta ttgctatcaa
    52441 tatctacctg caattgcggc atttgtggca aatcattggc gcgtacagaa tgtaactccg
    52501 gactttgatt cgctttttct atcaactgat tacgcatttg cagcagtgtt tcacgatcgg
    52561 ttccaccgtt agctaacaat tcaaacgtaa aaccattgct ttgccccagc ccatcaacgg
    52621 ctggcggcgt catcgcaaat accgtggcat cacgaattgt gcccagctct ttggttgccc
    52681 gtagggcgat agcctgggcg gtgttttctg cccctttacg ttgagaccag tttttcaacg
    52741 aaacaaacgc catcccggtg ttctgtccgc tgccgctaaa actgaaacca tcaacggtaa
    52801 agatgacatc ggtatttgct ttctcgttaa tcaggaacca gtcaacaatc tggcgattca
    52861 cttctgctgt acgggcagcg gtcgccccgg ccggcagcgt gtactgcacc atgatttcgc
    52921 cctggtcttc agtgggtaag aaactgcccg gcagtttcca catcatcaga gccattccac
    52981 cgcccagcac gacataaagc cccatcgttc gggctgcacg gcgcaggaca taaattacgc
    53041 ctcgctgata tttatcttca gtacggcgat aaaagcggtt aaatgcgccg aaaaagcctt
    53101 ttttatgtgg cggaacatgc tggaggacgg aaccgcacag ggcgggagtc aaggtcaatg
    53161 ccaccaccac ggaaagcagc attgcggaga tgatggtgat ggagaactgg cgataaatta
    53221 cgcccgtaga tcccccaaag aaggccatcg gcaggaatac ggctgacaac accagcgcaa
    53281 tggcaaccag tgcgccagaa atctcgccca ttgatttttc cgtggcttca cgcgcgggca
    53341 acccttcgtc acgcataatg cgttcgacgt tctctaccac cacaatggca tcgtccacca
    53401 gcaagccgat cgccagcacc attgcaaaca acgtcagggt attaatcgaa tagccaaaca
    53461 acgcaagaac gccgaatgtg cccagcaaaa cgacggggac agctaacgcc ggaatcagtg
    53521 tggcacgcag gttttgcagg aataaataca tcacgcaaac caccaatacg atggcttcaa
    53581 acagcgtctg aattacatcc tctacggaga ttttaataaa ttcagtgctg tctttcgggt
    53641 atgcgatgtc gtatccctgc ggcatgttcc gctggaattc ggcaatctta tccttgacca
    53701 gcgtcgccgt attcagcgca ttcgcaccgg gcgaaagcat tacagcaacc ccggcagacg
    53761 ggtgaccgtt aagtttgcca atagcggtat aatcttcact gcccatctca acgcgagcca
    53821 catcttttat acgaacaact gcgccgtctg actggctttt cacgataata tttttgaact
    53881 gatccaccgt ctgcaaacga gactgcgcac gtaccgttgc ggtcagttgc tgagtatccg
    53941 gtgaaggcaa tgcccctatt ttcccggcag taacctgcac gttttgcgct tcaatagcac
    54001 tttgcacgtc tgaaggcatc agcgagtaag acgccagttt ggccgggtcg agccagatgc
    54061 gcatagcgta ttccgcgcca aagacttgca gactccccac accttcaaca cgcgccagcg
    54121 ggtcctgaac gttactgacc agccagtcgg cgatatccga actggaggct ttgtcagtgg
    54181 tgtcatacac ggcagcaatc agcaaaaagt tgctttgtga cttctccacc gtaacacccg
    54241 tttgctgcac ttcgctgggt aggcgcgact ccgcctgctg aattttattc tgcacctgaa
    54301 cctgtgcagt atctggatcg gtaccttgtt caaaggtcac attaatactg accgaaccat
    54361 cagagctact ggttgagctg aaatagagta aattatcaag cccagtgagt tgttgctcga
    54421 taacctgagt cacgctgttc tccagcgttt cggcagaagc accagtataa gtggctgaaa
    54481 ttttaatggt cggcggtgca acgtcaggat attgcgcgac gggcaatgtg cgaatagcca
    54541 gaatccccgc caacataatc aaaatggcga taacccaggc aaagactggg cggcgaacaa
    54601 aaaagcgcga aaacatcagg cattgcctcc attagcctga acttcaacag ccttgacggt
    54661 ctgccctgaa gtgactttgg cagaaccttc aacaatcagt cggtcgccgt tgtgcaggcc
    54721 gttcagcacc agccatttat caccatacgt ttctcccgtt tcgagcgttc gctgctctac
    54781 tttattgtct ttattcacca ccagcgcatt tgcattgcct ttagcatcgc gcgtaacgcc
    54841 ctgttgaggc gcgagaatag cgttttccat cacgccttca tcgacacgag cgcgaacaaa
    54901 cattcccggt aataactgct gttgtggatt ggggaaaatt gcccgtaatg tcaccgaacc
    54961 ggtagactca tctacagcga cttcggtgag ttccagacgc cctttttcgc tgtaggttgt
    55021 gccatcttcc agaataagtg agacgctcat ggtgtcactg ttggtcgcca gtgactgttt
    55081 acgcagacgt aataaatcga cactggagcg agtgaggtcg acatacattg tatccagacc
    55141 acgaatagtc gttaacgctg tatcttgcga cgcggtcacc agtgcgccag gggttaccga
    55201 cgaaatgcca atgcgccctg aaatcggtgc ggttaccgtg gtccagtcaa gattaatgcg
    55261 cgcagtttcc agtgcggctt ttttcgcctc tacactggct ttatcttgtg cacaggtaga
    55321 ctgagcatca tcagcatcct gttgtgaaac accgttctct ttcaccagtc gggcataacg
    55381 ctgcgctttc tggcaatcgg cttttaccag tgcctgtgct tgttgtaatg ctgcccgggc
    55441 ttcattccat gcagcctgat aactggccgc atcaatctgg tagagaggct gtccagcctt
    55501 gaccagatca ccttccttaa ataagcgttt ctggataatt cccccaacct gcggacgtac
    55561 ttcagcactg agcgcagcac tggtgcgtcc ggttaattca ctgactaccg aaaccggttg
    55621 agatttgagt gtaacaacgc cgacttctgt ttctgacggg gatgaattgt tactttgcgt
    55681 atgcgtatta tcacaacccg ataagagcag cattgccact acagatgttg ctatatactt
    55741 cattattttc tccagggtga gaaataccct cctcattgaa attaatgagg atatgtcagg
    55801 gatattcagt tataacgaac ccgcatcgcg aaatgaattt cgcaatgaaa taagggggat
    55861 taatatcccc cgctattggt taattctgtt cttatttatt tatcaacata ctaacaatat
    55921 cctgatatat ttcctgcaga tgttgtggat cgcttttttg cggtgttaaa cgacgataaa
    55981 ccgtaccttc catcatggta gcaaacactt caacgcagca acgaatgtgc tcgtcgctaa
    56041 gatggggatg cattcttttc atatgagcac aaccgttagc gaacattctg gcgtcagctt
    56101 cttcaagcat tgccatcacc cgcgggttgc gtgtggcttc tgcagccact tccagcatca
    56161 gcgcgtcgtc gtcctcattt aaggatttac gcaaggcaag aacttccgga aagtgatcgg
    56221 tacgcgcgtc aatgtccatt tgggcaatac gaaagtcgat gatacggcga accatctctt
    56281 caataatggc gtctttgttg gcgaagtaac gatatatctg acccacgcta agctgagctt
    56341 cgctggcaat ttgcgacata ctggcggcat gaaatccgca gcgacgaaaa caccgacggg
    56401 cggcgttaat gatttcatcc tgacgctgtt tgtggcgtat ctccagcttg ctggtcattt
    56461 ctgtctcctg gcctatcact tggtgagaat gatcgttctc atttttgctg attatacgtt
    56521 tctccaaagt aatggaaagt atttttatct tattcatggt gcatattctg ataatgaaga
    56581 gattgtggag attctgtgaa gatagttgac aatattaagt tggggtcaat tcatcagaaa
    56641 ggcagcgact tgttgtcgcc agttctgttt tcatcatcaa acttatagga gctaatacga
    56701 atagtttcaa agtcagattc tataagatat ttccccaacc tcccccttgc aggcgtcagg
    56761 agtagataac gcaaaatgct ctgtaccgca gcttctctcc gctacagaaa gcgcaactat
    56821 agccacccac agccaggttg attataataa ccgtttatct gttcgtactg tttactaaaa
    56881 cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta aagctatggg
    56941 ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactgt ccatcgccta
    57001 cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct accagaacga
    57061 caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca tcgttctgca
    57121 gtgcgatgtt gccgaagata ccagcatcga caccatgttc gctgaactgg ggaaagtttg
    57181 gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc agctggatgg
    57241 tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gctcacgaca tcagctccta
    57301 cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt ctgccctgct
    57361 gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta tgggtctggc
    57421 aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt
    57481 gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg gtatcaaaga
    57541 cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta ccgttactat
    57601 tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg gtatctccgg
    57661 tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac tcgaactgaa
    57721 ataatcgttc tgttggtaaa tattgggcgg cgttctgccg cccgttatct ctgttatacc
    57781 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    57841 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    57901 ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc tggtcccgga
    57961 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag aaacgctggc
    58021 acccttcctt tcatggctgg tccctgcgcg cgttcttgca gtagagttgt tccctgacca
    58081 gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac
    58141 ggttgctcag gtttgcggcg tccaacggtt atgtaactac tattcggcgc gacttacgcc
    58201 gctccccggg cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    58261 tgcccgccaa ctggctagct cgccttctat tatcaacaac cgatcgcgcc agcatctgaa
    58321 tgacgttggt cttactgtct gtgactgtgt gatcattaac caaatcattg gttttattgg
    58381 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    58441 acccggtctg gagatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg
    58501 ctggcgaagc agctatgagg tggaaaaact ccctgaagag cacacaaaaa gttcaactgc
    58561 agaactttgc caactggcca acacactctc tctccaccct atttcacttt cccttctcga
    58621 aaagttgtta aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt
    58681 atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatgaatt catcaaatga
    58741 atataaaaaa atcagccccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
    58801 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    58861 tcgctttagc gccgcccagt tcagcccatt actcgaacac gaaaaatcat caacgcagat
    58921 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    58981 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    59041 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    59101 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    59161 ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa
    59221 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    59281 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    59341 aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc
    59401 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    59461 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    59521 ttggtgacga tcactttgta ccgtggtggg ttacccttgc gcgtcataat ctggaaaaag
    59581 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    59641 ctgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt
    59701 tcgccaaggc gttaccggat ggcaaacttc agctgattgt ggcgattgcc gatccaaccg
    59761 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    59821 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    59881 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc actgatggca
    59941 ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    60001 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa agcgaagcaa
    60061 ttgccgaaca agtccgtttg ctagcacaaa tttgccaacg ccgcggcgag tggcgtcata
    60121 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    60181 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    60241 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    60301 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    60361 cgcacggtct gcatgtcgat gctgaagaag tgctcacgct ggacggtttc tgcaaactgc
    60421 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    60481 catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    60541 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    60601 cggttatcaa aggcgaaact gcgacgcgtc cacaggatga aatcactgtc caaatggccg
    60661 agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    60721 tcctgaaaga caaagccggg accgacaccc gtttcgcggc ggaaattgtc gatatcagcc
    60781 gtggcggcat gcgtgttcgt ttggttgata acggcgctat cgcctttatt ccagcacctt
    60841 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    60901 aaggtgaaac ggcttacaaa gtgactgacg ttattgacgt caccattgcc gaagtccgca
    60961 tggaaacccg cagcattatt gcgcgcccag tcgcgtaatc tcctttcacg gcccattcct
    61021 catggatggg ccgtttattt ccccgctcaa ccttcgtcat atcccggcag atttttaact
    61081 gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat atataaaaaa
    61141 acaatgtgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt atttaactgc
    61201 ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt
    61261 gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgtttt
    61321 gcgcttttct accaccgaaa tcacagaacc cgatcgtatt ttgcaactat ctgccgaaca
    61381 ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt
    61441 cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac gagaatgggg
    61501 aggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat cacatgggct
    61561 ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg
    61621 ggggaatatc cagcgcttta atcggttatg tgaagattac acaggcttga aagaacacga
    61681 cgtcattggg caaagcgtgt ttaaactgtt tatgagtcgt cgtgaagctg cggcatccag
    61741 gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc
    61801 aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg
    61861 caaaaacgag atttttttaa tctgttccgg caccgacatt accgaagagc gccgcgctca
    61921 ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc
    61981 aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt
    62041 gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga
    62101 ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt
    62161 ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct
    62221 ggaagcattg gcatcacgaa ttttgacccg cttacgactc ccctttcgca ttggtttaat
    62281 tgaagtttat accagctgtt cagtaggtat ttcactctct cccgaacatg gttcggacag
    62341 cgcggctatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg
    62401 acaattttgc gtttttaccc cagaaatgaa tcagcgggta tttgaatatc tctggctgga
    62461 taccaacttg cgtaaagcac tggaaaacga tcagttggtt attcactatc aaccgaaaat
    62521 cacctggcgt ggcgaagtgc gcagtctgga agcactagta cgttggcagt cacctgaacg
    62581 tgggttgatt ccaccgttgg acttcatttc ctacgccgaa gaatcagggc taattgtgcc
    62641 tttaggccgt tgggtgattc tcgatgtcgt acgccaggta gcgaagtggc gggataaagg
    62701 cattaacctg cgagtggcgg taaatatttc tgcacgtcag ctcgccgatc aaaccatttt
    62761 caccgccctg aaacaggttc tccaggaact caattttgaa tactgcccta tagatgttga
    62821 actgacggag agctgcctga ttgagaatga tgaactggca ctgtctgtta ttcaacaatt
    62881 tagccgatta ggtgcgcaag ttcatctgga cgattttggt accggctact cttcactttc
    62941 gcaactggcg cgctttccga tcgatgccat caaacttgac caggtttttg ttcgggatat
    63001 tcacaaacag cctgtctcgc aatcactggt ccgggcgatc gtcgctgtgg cccaggcatt
    63061 gaatcttcag gtgatcgccg aaggtgtaga aagtgcaaag gaagatgctt ttttaaccaa
    63121 gaacgggatc aatgagcgac aaggattttt gtttgccaaa ccgatgcccg ccgttgcctt
    63181 cgaacgctgg tataaacgct acctgaagcg cacataagta gatgaatata tatacttagc
    63241 gctatattcc gtcattatat atcgtattcc cggatgataa ttagccgttg ctgttaaaca
    63301 cttctctttt agcggcaatt tttgcattat tcattttccc ccttttgcta taaggatgtc
    63361 cttgatgtct gaatttgatg cccagcgcgt tgccgaacgt attgatattg tgttagatat
    63421 ccttgtggca ggcgattacc actctgcgat ccataatctc gagatactta aagcagaact
    63481 tttgcgccag gttgctgaat ctacgccaga catcccgaag gcaccgtggg agatttaaca
    63541 atcgtgcctt caggttttcc tatttgagga tgcccggcgc gtcagtattt ttttatttag
    63601 tattataacg ttataagaat tacagcgatg aattcccgac aacaaactat tctacagatg
    63661 gtcattgacc agggtcaggt tagcgtaacc gatctggcaa aagccactgg agtttctgaa
    63721 gttaccattc gccaggatct caacaccctc gaaaaactga gttacctccg acgtgcacat
    63781 ggttttgcag tttcgcttga tagtgatgac gtcgaaaccc gtatgatgag caactatacg
    63841 ctgaagcgtg aactcgccga gtttgccgcg tcactggttc aaccgggcga aaccatcttt
    63901 atcgaaaatg gtagcagcaa tgccctgctc gccaggactc tgggcgagca gaagaaaaat
    63961 gtcactatca tcacggtcag cagctacatc gcgcatttgc tgaaagacgc gccttgtgaa
    64021 gtcattttgc tcggtggcgt gtaccagaaa aaaagcgaaa gtatggttgg ccctttgaca
    64081 cgccagtgca tccaacaagt gcatttcagc aaagcattta ttggtattga tggctggcaa
    64141 cctgaaactg ggtttaccgg tcgcgatatg atgcgtaccg atgtggtcaa tgccgtgctg
    64201 gaaaaagagt gcgaggcgat agtcctgact gacagctcga aatttggtgc tgtacattca
    64261 tactccatcg gccccgttga gcgattcaat cgtgtgatta ccgattcgaa aatacgcgcc
    64321 agcgatctga tgcatcttga acaaagtaaa ctcaccgttc acgtcgttga catttaacca
    64381 tttcccttct ctacggatga tttgcagttt ggcaaatcat ccgctctaag atgattcctg
    64441 gttgataatt aagactattt acctgttatt aacactctca agatataaaa ttattatcag
    64501 cgatataaca ggaagtcatt atcacctgcg tgatataacc ctgcgcgcga gcggatttca
    64561 cggaataatt tcaccaggct tattcttagc tattatagtt atagagagct tacttccgtg
    64621 aatcataaat tcaggagaga gtattatgtt tgtaacgagc aaaaaaatga ccgcggctgt
    64681 tctggcaatt accctggcaa tgtctctgag tgcctgttct aactggtcta aacgggaccg
    64741 caacaccgca atcggcgcag gtgcaggggc attaggcggt gcagtactga ccgatggcag
    64801 tacgttgggt acattaggtg gtgcagccgt cggtggtgtt attggtcacc aggtcggtaa
    64861 ataatcgtaa ataaatgtcg attatcgact cgcaattata atacgcttga ttctgttcag
    64921 tacgaaataa ctgaataata cacacagtga aatattcgaa agccacggat atatccgtgg
    64981 ctttttatgt tagccgcctg cgagttttac cttcatccct ttcgcttcaa gcagtgattt
    65041 taataaatca cgcttatcgc cctggatttc aataattcca tctttaactg ctccgccgca
    65101 gccgcatttt ttcttcagtt ccgctgccag tttcgtcagt tcggcatcat cgagagcgac
    65161 accggtaatc aggcaaacgc ccttaccttt acgtccactg gtctgacgct gaatacgcac
    65221 cacaccgtcg cctttagggc gtacaggggc cgcttttggc tcatcaatac gtccggtttc
    65281 cgttgagtag accagacggc tgttggaatc actcatcatg cactccgctg taaagaggcg
    65341 ttgatcgctt tcagcgtctg cgctggatct accgattgcg ttaccgggcg accaatcacc
    65401 atataatcaa caccagccgc caacgcctgt tctggcgtca taatgcggcg ctggtcacca
    65461 gcatcactcc cctgcggacg aatgcccggc gtaaccagtt tgaactcctg accgaatacc
    65521 tgtttaaagc gcacagcttc ctgagcagaa cacaccacac catcaaggcc acatttttgc
    65581 gtcagtgccg ccagacgttc tgcataatct gcaggtgaca gtgtcacgcc aagatcggcc
    65641 aggtcgctgg cttccatgct ggtcaacact gtcacagcaa tcaaaagcgg tgcatctttg
    65701 ccaaacggaa ccagtgcctc acgcgctgcg gccatcatac gcgccccacc ggaggcatga
    65761 acattcacca tccacacgcc taagtcagcc gcagcagcga cagcgtgcgc tgcagtgttg
    65821 ggaatatcgt ggaatttcag gtcaagaaag atatcaaaac cacgctgttg aagttcgctc
    65881 acaaactgtg gcccaaacaa tgtaaacatc tctttgccga ccttcagacg acaatcgcgt
    65941 gggtcgatct tgtcgacaaa ggccatcgcg gcatcgcgat tatgataatc aagggcaaca
    66001 actacaggag aattcgtaac agcgcgggaa gaagatgaag cagttaacgt catgaccaga
    66061 ccttcttgat gatgggcacc ggaagtggcg cgaaacaggt aaacggcgag cattctaccc
    66121 gtggaaataa aaaattaaca gttgcgattt ctcctctggc aggcttttcc gcgccgtcgg
    66181 tgccggagca caatagaaca tcaataatta gtatgttgta actaaagtcg gattttttta
    66241 aaaattacag gccatcaaga ccgcgaatcg gtttaatggt tgaccaggcc cgacaagacg
    66301 gacaatgcca gtagagagtg tatgcggtaa aaccacattt ctggcagcga taacgaggct
    66361 tactacgcac cttctcgcca accatgtcac gcagcaccat cagactctct ttggcacgcc
    66421 cttcttctgc ttcatttaag tggtaatcca ttaacttatg gaacacacgc atggtcggat
    66481 gacgctgaag ctggcgcgta atatagacct gtgcggcctc actaccgtcg cgcgcttcga
    66541 tgatatccgc aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgctgca
    66601 ggaattctgc ccattcggca gttttaccca actgctgata gcatgtttgc agcatttcca
    66661 gcgtttcgct gaccagttct ctgtcctggg atatgacacg ttgcaaactt tcgacggctt
    66721 tggcgtattc tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt
    66781 ttttatctgc cgccgcacct ttttttagca aagtcatggc acgatcgaga tcgtcgctgg
    66841 ccatatgctg cagagctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt
    66901 tacccagctt caccaggcgt tcggcaacat caattgcttt ctgccactcg ctggtagcct
    66961 ggtaaatttg tagcagttgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct
    67021 gattgaacat gtcttccgcg cggtcgtata acccggcagc catgtaatca cgccccagtt
    67081 gttgaatcgc caaaagccgc tgttcatagg tcagcgaggc gctttccatt agggtctgat
    67141 ggatgcgaat agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt
    67201 gggcttcaac ggtacctgta tcctctttaa gcatatcgag aaacaggtct accgctttat
    67261 cctgttgatt actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg
    67321 cttcatcttg cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg
    67381 ctacaggcaa aagcagaaac aacaactcca gcatagaaag ttattccttc gacgcagacg
    67441 agtgcggcgc tacagccacg tcagtcgcag gtgaaagctg gttttccagt cgctttattt
    67501 tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc
    67561 aaccgatagc aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc
    67621 cttgcgctaa cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg
    67681 aaatcacgaa gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg
    67741 ttatgtggtt agggcgaata aattatggtc aacttaccgc aatagccctt taaagtacca
    67801 ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa gggctaaatt
    67861 cggaaaatca accagcgtta actttcttgt tctcgttgtg caatttcgcg attttcttcc
    67921 gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc
    67981 gcccacgaaa tcaacgtagc tacaaccaga tcgcgtgacc aatgcatccc gagcagcagg
    68041 cggcttccca tgactcccgt tgcccagacc aacaagatag caatggttaa cgttcgccga
    68101 cgcggccaca gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg
    68161 gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tgggatattt
    68221 ttctcttcag ccaactgttc tttcaccaga tttccgcgtt ctgctcgctt taaagtgtag
    68281 aactcatcaa ccggaatatg atgtgttttt tccagccaga taacaaaagg tcgtggttcc
    68341 tggactttgt ctttgatcca ggatttaacg ccttgtccca taaggattgc cgccgccaga
    68401 atggcaaata acataatggc agccttaatg cgaaaacgca gacaccagag aaaccagccg
    68461 aataaaatca aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa
    68521 gccgctttta gtaaccaact ttgttctcca ggttgccaac gccagccaga aagccatacg
    68581 gctactggca tgacaagcaa tagtgcagct cccactgcgg tacgtctggc aatcgaacgc
    68641 atggcctctc ctttttgata agtcccacaa tcataactga aaatgccagt tccaggaaaa
    68701 attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc
    68761 tcgttatggc aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt
    68821 aaacgtgtgg cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt
    68881 gaagaactgg caaccggaca cgatcatgtc gcgctagtct atggcgatat ttccgggcat
    68941 accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg
    69001 cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt
    69061 ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc
    69121 gcttacgcac tgcaggatca aggttacgat actgtagagg ctaaccacca gttaggcttc
    69181 gccgctgatg agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat
    69241 gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga agcagggatt
    69301 aatattattg aacgcgtacc gttgattgta ggtcgtaacc ccaataacga acattatctc
    69361 gataccaaag ccgagaaaat gggtcatttg ctgaacaaat aaccctcttg cattgtgtaa
    69421 ttaatttgct tgccggaagc aaaataaccg gcaagcaaat actcgttact tcaacatatt
    69481 acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag gccaactccg tcgcggtgtc
    69541 gatacgacaa cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg
    69601 aaccgtcgcg ccgggttgta gattttgcag atcaccaata tcaatcttct cttccccggt
    69661 aagctctaac gttttacgcg tcacaccttg tggaaattcc agcggcagga tgcccatgcc
    69721 aattaaattc gaacggtgaa ttcgttcaaa cgattcggca atcacaacac gaataccaag
    69781 cagacgcgga ccttttgccg cccagtcacg gctggagcct gatccatact ctttcccggc
    69841 aatcaccgcc agcggcgttt gctcctgctt atagcgcatt gcggcatcat aaatagagac
    69901 gacatcgctg tcaggtaaat ggcacgtcat ccccccttca acgccaggca ccatttcatt
    69961 acggatgcga atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga
    70021 accataggag ttaaagtctt tccgctcaac accccgacct tgtagatatc gacccgccgg
    70081 gctgtcgggc ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag
    70141 cattgcgagg atccgcgcac cgtgaatatc ttccacaggt gctggtgttg cctgcatttc
    70201 atcaaagaaa ggcgataagc gaatataggt tgaatcctcc tgccaaccgt aggtatcgga
    70261 tcgtgtgacg ttaatttcct tccactcagc tgtgccttca aaaacttctg cgtactcttt
    70321 gcggaacatt tctgtggaga cttggtctac cgcacgggca atttcttgtg ccgatggcca
    70381 gatatctttc agatataccg gctcgccttt gcgatcatgg ccgataggct cagaagccag
    70441 gttgatattc atatttcccg ccagcgcata ggcaaccacc agcggcggcg aggccagcca
    70501 gttggtttta accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcactgc
    70561 accgacggtt aaatcgcctt ttttgattgc cgtttcgata ggatcgggca acggtccaga
    70621 gttaccaata caggtggtac aaccgtatcc cacaaggtta aatcccagtt catcgagata
    70681 cggtgtcagt ttcgcttttg ccagataatc agaaacgact ttcgaacccg gtgccagcga
    70741 cgctttgacc cacggttgcc gcttgaggcc cagagttacg gcttttttcg ccagcaagcc
    70801 tgcggccatc agcacacttg ggttagaggt gttggtgcac gacgttatcg cagcaatgac
    70861 cacagcgcca tcaggtaact gatactgatg tccgttcata acataatcga ccggcaggcg
    70921 atctttatgc gtggcattca cttccagttc gctactggca gcaaatgctt ttggtacatc
    70981 gggcagtgca acgcgatcct gcgggcgttt tggccctgca aggctcgctt caacgtcatt
    71041 catatccagt tctaacacac tggtaaaaat tggttcatcg cccgaattac gccacatgcc
    71101 ctgcgctttg gcatattttt cgaccaactc gacctgatct tcactgcgcc cgcttaaacg
    71161 catgtaatcg agggtgacag catcgattgg gaagaagcca caggtggcac catattctgg
    71221 tgacatattg gcaatagtgg cgcgatccgc cagcggtagt gaatccagcc catcaccata
    71281 aaattcgacg aatttcccca ccacgccatg tttgcgcaac atttgggtaa cagtgagaac
    71341 caggtctgtg gcggtaatac cttcgcgtaa ttttccggta agtttaaagc ccactacgtc
    71401 cgggataagc atggaaaccg gctggcctaa cattgcggct tctgcttcga tgccaccaac
    71461 gccccacccc agcacgccaa ggccgttgat catggtggtg tgcgagtcag taccaacgag
    71521 tgtatccgga taagcaatcc attcaccgtc ctgcaattcg ctccacactg ctttgccgag
    71581 atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact
    71641 gaacgcctgc tttccccatt tcaggaacac ataacgttcg tggttgcgct ccatttccag
    71701 gcgtacgttt tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc
    71761 aatgaccagg tcgaccggtg agagtgggtt aacctttgca gtatcgccgc cgaggcgttt
    71821 aaccgcttcg cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat
    71881 cagcactctt gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc
    71941 cgccagcgcg tggatatcct cttcggtaac cgatttacca tcctgccaac gcagcaggtt
    72001 ttcgagcaaa actttgagtg acttgggtag acgggtgata tcgcccagtg atttggcagc
    72061 aagcggcaag ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt ccttactggc
    72121 ttctcgtagg gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct
    72181 cttcagggtg aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa
    72241 ttgataaaag aggagaaacc ttgaggcaaa gcaaacagcg gggtcaggga tcagtgagta
    72301 atgatccaga ggagttcggc acagaagagc atcgacagag aaaatgcgcc catccaggac
    72361 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ctttgtgttg
    72421 agataactca acctgttatt gctgcggtgt attaaaagcc ctgtaattca ttaaaaatgg
    72481 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    72541 aggcttgttg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    72601 ttgcttctgg ttcattgtca ctatgctgac ataatggtga cgactccagt tgctcctgac
    72661 cgaccatagt tatccccaca atttccaaat aagcagtgcg acaaccaccc agaaaagtgc
    72721 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggattgc ggcggggttc
    72781 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    72841 gaacgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    72901 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    72961 tttgaaaact ggagtttgcg aggtgtgtaa ttagacacta ctttacaggt aatttaccct
    73021 ataaatatga gggataaaaa tattgtgcac cggatacaaa taagtaacaa aggagaatat
    73081 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    73141 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
    73201 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    73261 acgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatgtcgtg
    73321 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    73381 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    73441 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    73501 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    73561 tttacctgcc agcgggtgat ccggagtgac tactatagcc cgattccagt ggtagcacgg
    73621 tagcatcact aaatcttcat acagatgcag cgcttccgtg gcgatagcga aatcggcatt
    73681 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
    73741 gcgaggataa cgctcaataa agcctttgat aacgtttggt aacgcatagc gtgcctgagt
    73801 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctctc cggcgaccga
    73861 ttttatggca tcgactttcg acagaacttc acgagcgata cgaattattt cttgtcctgc
    73921 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    73981 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgccgt
    74041 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    74101 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac accaggcggg
    74161 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    74221 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    74281 ctttgtttat aaaaaccaga cagaattaaa ggttattttt tcccttcaac ccatttgcca
    74341 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    74401 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    74461 gcatcggcca gataacgcag tttttccggc agacggccgc ggaagcgata aagctcctcc
    74521 accagcggcg cacgcgtttc acgcgatttc gggaaggtat tggccgccag gaacacaccg
    74581 gcagcaccgt cacgcaacac gaaataggca tctgattttt cgcacggcag ctccggtaat
    74641 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
    74701 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    74761 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattca
    74821 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    74881 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    74941 cgcaacgcat tggtttcagc gtcttcacct tccagcacgt tcagcacttc gttttccggc
    75001 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    75061 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cggacagtca
    75121 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    75181 ttatctaatt gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    75241 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtgaagtc gtagttcatt
    75301 aactcgcgga aattctcttc caggcgatcg gtgacgattt cgcccatttt ttccgcatag
    75361 aaacgacgat tttctactcg cacatagcca cgatcctgaa tagtcgaaat gatcgacgca
    75421 taggtagaag gacgaccgat accgcgtttt tccagctctt tgaccagcga tgcttcactg
    75481 aaacgggctg gaggcttggt aaagtgctgg gctggtgtca gttcaacgag cgtcagagca
    75541 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    75601 actttcgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    75661 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
    75721 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt caacgattcc
    75781 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    75841 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    75901 ccgcgaacca tatttaccgc gtcctgactc aggttggtgg agtcggtacg catgtaagtg
    75961 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    76021 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    76081 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    76141 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
    76201 tgcaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
    76261 tccggtacga acgctttaat ttcacgctca cgctcgacca ccaggcggac cgccaccgac
    76321 tgcacacgac cggcagacag accacgagcg atctttttcc atagaagcgg cgaaaccata
    76381 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    76441 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    76501 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    76561 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    76621 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    76681 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    76741 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagttgagcc actggtcggc
    76801 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
    76861 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    76921 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    76981 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gctcacgaaa
    77041 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    77101 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    77161 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    77221 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    77281 gagtaaataa tgaacaaaga gactcaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    77341 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    77401 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattacca
    77461 actgccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    77521 aaatatcact tggttgatta aagcaaaacg cgaggtctta tcctcgcgtt tgtcttttac
    77581 atcaacgact tttgcccacg ctgccaccag cgtaacaaca atcgatcggc gctctctgcc
    77641 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attgaccact
    77701 tcccggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    77761 cctttctcta ctgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    77821 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcaactct
    77881 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaacgtcag cgtgcgctta
    77941 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    78001 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    78061 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    78121 gttaaaggaa tgtttttatc acgcagacgc tgcaactgcg aagccgccaa cccgtaacca
    78181 tgcaccatgc cacctgggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    78241 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    78301 ttaaaatcca gcacccagac gcggggttta ctgtcagtta ccacctcgcc cagtttagct
    78361 ttcgcttttg cggctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    78421 ttttgctgat gagtgtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    78481 tcgctgagat tgttgactcg taactcgcca cgctggcgtt tattacgttg agcaacattg
    78541 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
    78601 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    78661 acagtttaca acaggcatgt ttagccgtct cgcagagatg ttattgcagc gaaagtatga
    78721 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    78781 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    78841 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    78901 gagccagcga tggaattggt cgtgaagcag cgatgacgta tgcacgctat ggtgcgacag
    78961 tgattctttt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    79021 aaattggacg tcagccacag tggtttattc tcgatttgct aacctgcacg tccgaagatt
    79081 gccaacaact ggcacagcgc attgccgtta attatccgcg tctggatggt gttttgcata
    79141 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca agatccgcag gtctggcagg
    79201 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac acaggcactg cttcctttat
    79261 tactcaaatc ggatgccggt tcactggtct ttacctcatc aagcgttgga cgtcaggggc
    79321 gtgcaaactg gggcgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    79381 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    79441 ccgcaatgcg ggccagcgcc ttcccgaccg aagatccaca gaaacttaaa acccccgctg
    79501 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    79561 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    79621 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    79681 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    79741 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    79801 cacctggcct aatggtgaac gaaatctgct ggagccacac ggcgttgagt ttcaggtaat
    79861 ggcaacgggc tttacctggg atacacaaaa ccgagagtct gataccgccg cctgccgcga
    79921 agtctggcaa catgcaaagc ggatgctggc tgattcctca ctggatatgg ttttgctcga
    79981 tgaactgaca tatatggtag cgtatgatta tttgccactg gaagacgtga ttcaggcgtt
    80041 aaatgaacgt ccacatcaac agacggtgat tatcacaggt cgtggttgtc atcgggatat
    80101 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
    80161 tgtaaaagcg cagataggga ttgattacta atctgtaaaa atgccggata tgacgtccgg
    80221 catactttga ttaaccgtta ttattacgtc caccagaacg acgaccgccg ctcacctggc
    80281 tgtgacgttt caccgcacga cgaatttgat tcgccttcat acgacgacgg tctttttcta
    80341 cagcgacttt agagctggtt tctggcggta gttccaccag ttcgcgcaga tagttagtct
    80401 gggcgaggtc cagctccgtc cagccaccgc gtggcagacc ttttggcagt gggatatcac
    80461 cgtaacgaac acgaatcagg cggctcacct gcacaccaac cgcttcccaa agacgacgaa
    80521 cttcacggtt acggccttcg gtcagagtaa cattgtacca ctggttgatc ccttcgccgc
    80581 cgctgaactt gatggtttta aaggctgccg gaccatcttc cagctgcacg ccacgactca
    80641 aatcacgcag tttcgcgtcg tcaacctgac caaatacacg cacggcatat tcacgttcaa
    80701 cttcacggct tggatgcatt aaacggtttg ccagttcacc gtcggtggtg aacagcaaca
    80761 gaccacaggt gttaacgtcc agacgaccta cggcaatcca gcgtgcgcca cgcagttttg
    80821 gcagacggtc aaacaccgtt ggacgccctt ccggatcgtt acgggtacac aactcacctt
    80881 ccggtttgta ataggccaga acgcgacaaa tttgttcagc ggactcacgt accgaaatca
    80941 gatgaccatc gatacggatt ttcagtccag gggtaatttc aacgcgatcg ccgagtttag
    81001 caattttgcc atcaacactc acacgaccgg cttcaataat agattcgatt tcacggcgag
    81061 aaccgtggcc ggcacgcgcc agcactttct gtagcttttc gctcatagag cttccttttg
    81121 gtgtcgcctt cccaggcgtc tgatatgctt ttgtgcaaca atgagtcaga acttacatct
    81181 gactcactga tagtaaagtc tgtttgaact cttcaggcct taagtaaacg actcgacgtt
    81241 ttaacttgcc tggctttgag gcgcaacata ctacactatc tttatggtta aagcttgttt
    81301 cgattaacat cagcttgatt accagccacc accgcctccg ccaccagcgc cagaaccacc
    81361 accagaatag ctccgcccca acaaactggt atagaccccg caactcaggt gcgtctgtaa
    81421 tgagaataaa acgccgttac gggcaatctc tggagcgcaa atcgccgaag aaagcgaagg
    81481 ggcgatgcgt aacgcccatt ccctccccaa atttgccgat atagcccaac ctaacaacgc
    81541 gtaatccagt ttccgtgttt cccctttcct ccgccgccca ctgtgtgttg ctgcctcttt
    81601 acgcgcaaga tagttaacga tagcttcacc gtgggcataa cgttgctgcc cggtttgggt
    81661 aaactttggc agaaaaatat accccatgcc agtgagataa ccggtcagga aaaccgcgct
    81721 aaaataaccc gctggcatat accagtaaaa gaatacatct ccgacattga tgtagagaga
    81781 gtaaaaagcc acacctgaac aaatcagtgg taaaaacaaa gtgatgatga cacgattaac
    81841 gcaaggcttg tcatcatcgc gggcaaacaa aagatcaagg gaacaaaata agaaagtaag
    81901 aagccagcag atcataataa acatatcgcc taccagactc attccttccc agcctacggg
    81961 actcaggtta ccgtagagaa tgaccgccaa tattgccatt ccgccccatt gcaaaaacgc
    82021 atcaggtcgg taaaacgaag actttttacg cggcagataa aatttctgca tccgtaaaaa
    82081 tgccttgcgt aagactctgt tgcgtctccc cgtaagaacg accttatcgc ctttacgaaa
    82141 taacgcttcc agtaataact gctctgcggg cagtaatacc gcattatttc tggtcgcgcc
    82201 ttcattaaca cgaatcagga tttgctggtt cttgtcataa tgatcttcca gatgaatttt
    82261 ttctttcaca atcagattta cgatatcggc gcaaaaacct ttatcgtcgt aaaccagttt
    82321 cgcatcaaga cgtaacatgc cgggcgtgta atctggcgga atgacatcgg tttcaatcac
    82381 atctaccggc gtaaattgcg gcctgcgctt ccagagatat aaccatccac acgccaggaa
    82441 cagactcgga aagcaaatta acaacgatga cgttgagggt aaaagaagat gactgaaaat
    82501 attcgtcgtt tgtggagccg gggcattacc aagtaatgcg tgaggccagc ggtagaggac
    82561 cgtgaaatct tcacgataaa acggatcgcg agattcaatt cttccgtcct caaggatgcg
    82621 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
    82681 acccgcaata tcaggcaact tgagtgaaaa tagggtctta taaatttcaa acggccagtg
    82741 gttaccggtc acgttccaga ttagcagatc agaatccccc tcacgcagga aagcattttt
    82801 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
    82861 cacgacaata ctcattaacc cgatatgcca gtcaagatgc cagggttcag gaataccatc
    82921 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
    82981 gagtcgcggt aaatcacgat aaaatccgtg cttaatctgc tgatttcgcg cctgaaaatg
    83041 aatgttctct ttgatgtcga gcaccccatt cggttgcaga gtggcttcaa catcaaagag
    83101 cgagatccct tcttcataag aagggatctc cgtgtttttc gcataactca acgaagagaa
    83161 aaaaagccca actatcaaca gcaaaatgca gcgatatatc cctgccatca tcttccatcc
    83221 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
    83281 cggcgtatca tcggtgagat caataaccgt tgttggtttc tgcccgagat agccgccgtg
    83341 aataatcaaa tccacctgtt tttccagacg gtctttaatt tcttccggat cggattcggt
    83401 aaattcactg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca atgcttcaag
    83461 cagcgcctgc gcaatcgggt tagacggcac acgcataccg atggttttgc gtttttcctg
    83521 caacagacgg cgtggcacct ccttcgtccc cttcaggatg aaggtatagt tgcctggcgt
    83581 gttgtttttc atcaaacgaa acgcaacgtt atcgacaaat gaataggtcg agagttcaga
    83641 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    83701 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    83761 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca ggcgttgctg
    83821 cgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    83881 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgaccc
    83941 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    84001 tgacgcgcaa gtgtcgccag ctgggtgcgt tcattgggcg attgctggca ctgcgcgact
    84061 tccatcgcgt cgccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    84121 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    84181 tcaatagatt gctctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    84241 agatactttt taaagacatc cgccattgaa ctggcttttc cgcactccac aaggaaacgc
    84301 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gttgcgcgct ttccagtgcc
    84361 ccaggtattt gcgctttttc aagtctttcg gcaatcagct gcgcccgctg attgcgccgt
    84421 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    84481 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacaccagg aataagattc
    84541 agcgccagtc ccgaacgtga aatttcctct cttgctggtg caattgcagc tgtggtgtca
    84601 tggtcggtga tcgccagggt gccgacgcgc atctcgactg cacggtgcac caatgcttct
    84661 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    84721 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    84781 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcgaact agttaactag
    84841 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaaggttg
    84901 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    84961 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    85021 aaccgactct cgaactgcta acctgcaaag gcgcttatcg cgacaacccg actgcgcttt
    85081 ttcaccagtt gtgtggggat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    85141 gcaaagatga tttaaaaagc ctgctgctgg tagacagtgc gctgcgcatt acagcattaa
    85201 gtgacactgt cacaatccag gcgctttccg gcaatggaga agccctgttg acactactgg
    85261 ataacgcctt gcctgcgggt gtggaaaatg aacaatcacc aaactgccgc gtactgcgct
    85321 tcccgcctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    85381 acgctttccg cttattacag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    85441 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaaaattta ccgcaactgt
    85501 cagcggaaaa tagctgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    85561 accatcagaa aaaaagcacc cgtattcagg ccagcctgtt tgctccgaat gaagaagaaa
    85621 aacaacgtct cactgctcgc ctgaacgaac tacgtcagca actgaccgaa gccgcgccgc
    85681 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taaccagagc gatgaagagt
    85741 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc cggagaaatt ttccaggtgg
    85801 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    85861 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    85921 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatttacc
    85981 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ttcactggat agagacctcg
    86041 acagccgcat cgaactggag atgcgtaccg atcataaaga gctttctgaa catctgatgc
    86101 tggtggatct cgcccgtaat gacctggcac gcatttgcac acccggcagc cgctacgtcg
    86161 ccgatctcac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgttgttg
    86221 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    86281 cgttaagcgg tgcaccgaaa gtacgcgcta tgcagttaat tgccgaagca gaaggtcgtc
    86341 gacgcggcag ctacggcggc gcggtaggtt attttaccgc gcatggctat ctcgacacct
    86401 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    86461 gcgtagtcct tgattctgtt ccgcagtcgg aagcagacga aacccgtaat aaagcccgcg
    86521 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gacgttctaa tggctgacat
    86581 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    86641 tggtcataac gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    86701 ggcgacgatg agcaatccgg tgctgatgct ttctcctggc cccggtgtgc cgagcgaagc
    86761 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgccaatta ttggcatttg
    86821 cctcggacat caggcgattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    86881 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    86941 aaacccgctg ccagtggcgc gttatcactc gctggttggc agtaatattc cggccggttt
    87001 aaccatcaac gcccatttta atggcatggt gatggcggtg cgtcatgatg cggatcgcgt
    87061 ttgtggattc cagttccatc cggaatccat tcttactacc cagggcgctc gcctgctgga
    87121 acaaacgctg gcctgggcgc agcagaaact aaagccaacc aacacgctgc aaccgattct
    87181 ggaaaaactg tatcaggccc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    87241 ggtggtacgt ggcgaactga aaccggaaca actggcggcg gcgctggtga gcatgaaaat
    87301 tcgcggcgag cacccgaacg aaatcgccgg agcagcaacc gcgctactgg aaaacgccgc
    87361 gccgttcccg cgcccggatt atctgtttgc cgatatcgtc ggcaccggcg gtgacggcag
    87421 taacagcatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgcg ggctgaaagt
    87481 ggcgaaacac ggcaaccgta gcgtctccag taaatccggc tcgtcggatc tgctggcggc
    87541 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    87601 cgtctgtttc ctctttgcac cgaaatatca caccggattc cgccatgcaa tgccggttcg
    87661 ccagcaacta aaaacccgca cactgttcaa tgtgctgggg ccattgatta acccggcgca
    87721 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    87781 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    87841 ttcattacac gcgccgacaa tcgttgccga actgcatgac ggcgaaatta agagctatca
    87901 attgaccgct gaagattttg gcctgacacc ctaccaccag gagcaactgg caggcggaac
    87961 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgctca
    88021 cgaagcagcc gtcgcggcga atgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    88081 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    88141 caccgcactg gcggcacgag ggtaaataat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    88201 caaggcgatt tgggtagaag cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    88261 gattcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    88321 tctggagtgc aaaaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    88381 acgcattgct gccatttata aacattacgc ttcggcaatt tcagtgctga ctgatgagaa
    88441 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    88501 tttatgtaaa gacttcatta tcgatcctta ccagatctat ctggcgcgct attaccaggc
    88561 cgatgcctgc ttattaatgc tttcagtact ggatgacgaa caatatcgcc agcttgcagc
    88621 cgtcgcgcac agtctggaga tgggtgtgct gaccgaagtc agtaatgaag aggaactgga
    88681 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgcgattt
    88741 gtcgattgat ctcaaccgta cccgcgagct tgcaccgaaa ctggggcaca acgtgacggt
    88801 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcactaa
    88861 cggctttctg attggttcgg cgttgatggc ccatgacgat ttgaacgccg ccgtgcgtcg
    88921 ggtgttgctg ggtgagaata aagtatgtgg cctgacgcgt gaacaagatg ctaaagcagc
    88981 ttatgacgcg ggcgcgattt acggtgggct gatttttgtt gcgacatcac cgcgttgcgt
    89041 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    89101 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    89161 gcaactgcat ggtaatgaag atcagctgta tatcgacact ctgcgtgagg ctctgccagc
    89221 acacgtcgcc atctggaagg ctttaagtgt cggtgaaact cttcccgcgc gcgattttca
    89281 gcacatcgat aaatatgtat tcgacaacgg tcagggcggg agcggacaac gtttcgactg
    89341 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    89401 taactgcgtg gaagcggcac aaaccggctg cgccgggctt gattttaatt ctgctgtaga
    89461 gtcgcaaccg ggtatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    89521 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    89581 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    89641 gcgcaaaaag atcctgaatt tcaggctcag ttcaacgacc tgctgaaaaa ctatgccggg
    89701 cgtccaaccg cgctgaccaa atgccagaac attacagccg ggacgaacac cacgctgtat
    89761 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    89821 gctttactgg cgaagcggat gggtaaaact gaaattattg ccgaaaccgg tgccggtcag
    89881 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    89941 ggtgccaaag acgttgaacg ccagtcgccc aacgttttcc ggatgcgctt aatgggtgcg
    90001 gaagtgatcc cggtacatag cggttccgcg accctgaaag atgcctgtaa tgaggcgcta
    90061 cgcgactggt ccggcagtta tgaaaccgcg cactatatgc tgggtaccgc agctggcccg
    90121 catccttacc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aacgaaagcg
    90181 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggtggt
    90241 tcgaatgcca tcggtatgtt tgcagatttc atcaacgaaa ccgacgtcgg cctgattggt
    90301 gtggagcctg gcggccacgg tatcgaaact ggcgagcacg gcgcaccgtt aaaacatggt
    90361 cgcgtgggca tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcaaatt
    90421 gaagagtctt actccatttc tgccgggctg gatttcccgt ccgtcggccc gcaacatgcg
    90481 tatctcaaca gcactggacg cgctgattac gtgtctatta ccgacgatga agccctggaa
    90541 gcctttaaaa cgctttgcct gcatgaaggg atcatcccgg cgctggaatc ctcccacgcc
    90601 ctggcccatg cgctgaaaat gatgcgcgaa aatccggaaa aagagcagct actggtggtt
    90661 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    90721 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    90781 gcgcattcgt tcctttcgtc accctcggtg atccgggcat tgagcagtcg ttgaaaatta
    90841 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    90901 cactggcgga tggcccgacg attcaaaacg ccacactgcg tgcttttgcg gcgggagtaa
    90961 ccccggcgca gtgctttgag gtgctggcac tcattcgcca gaagcacccg accattccca
    91021 tcggcctttt gatgtatgcc aacctggtgt ttaacaaagg cattgatgag ttttatgccg
    91081 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgcccgtg gaagagtccg
    91141 cgcccttccg ccaggccgcg ttgcgtcata atgtcgcacc tatctttatt tgcccgccga
    91201 atgccgacga tgatttgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    91261 tgtcgcgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    91321 ttgcgaagct gaaagagtac aacgctgcgc ctccattgca gggatttggt atttccgccc
    91381 cggatcaggt aaaagccgcg attgatgcag gagctgcggg cgcgatttct ggttcggcca
    91441 tcgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    91501 cttttgtaca accgatgaaa gcggcgacgc gcagttaatt tgagacaaac cgccagaaaa
    91561 catctggcgg tttattgatg gtaaaaaatt agaagcggta acccgcagag aacataaata
    91621 cccacgggtc aaggcgtacg ttgtcagaat aacgccctgc aggcagcact gcgccgttgg
    91681 atttatattt cacttcggta tcgatatcca tgtaccagac cggtagatta taaaaaatgt
    91741 aataacaagc tgtttataaa aggaaaaaca accttttgcg atgatttttt atacagttac
    91801 tctgtgtttt taaccgcttg atttttttaa agtctttttt tgttttgcag aggattatcc
    91861 cttcccttat agatcatttc ataaacaacg gatagtacaa tgtacacacg attgtcaaaa
    91921 ttcaccaatg atgggctcac catcttaaga gccgtagaca aagatactgc gcaagagttc
    91981 gttcatgaaa tgctgcagta agtctgagaa actcgaatgc cgggtttatt cagtaaaatt
    92041 ttctgctcat tgttttgtta aatagctcag aaacgacttt ctataccaga ttattttatt
    92101 aatacagacc cgacaatgtt attcattaat aaatacaaag ccgggtctga ctttgcacta
    92161 tttatatttt aaagcatcag gaccacgagc ctgtgccacc tgcaacaccc gatactgttg
    92221 acacaccacc tccctcgaaa tgccccaaaa gatttgatgc agaatcaaaa aacaatacac
    92281 tacaaaatac aggggtcatt gttatctgga agctaaccgt atttgtatac agatcatcca
    92341 aatcatctgt atataagtca ccaaacagca caccaccacc tggagatgat aatcctcctg
    92401 catttcccga aaaatgtttt ccaccttcac tggtgatctg agtgtctact tttatataaa
    92461 atattgcact agctaatgta gtttttgctg ggtatttatt ttctgccgca gctatcttgc
    92521 ttgtaataca agatacttta tccttatcaa gatattctcc atatcttctt tgtacatcat
    92581 ccataatctg ctgtctaagg tttgaacatt tagtcatagt agtcaccatt aaaagtaatt
    92641 atgggatt
//
LOCUS       DAZFRE010000004        87540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFRE010000004 DAZFRE010000000
VERSION     DAZFRE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.004"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(262..1662)
                     /gene="asnS"
                     /locus_tag="ACHKUI_000330"
     CDS             complement(262..1662)
                     /gene="asnS"
                     /locus_tag="ACHKUI_000330"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6596601.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1831..3033)
                     /gene="pncB"
                     /locus_tag="ACHKUI_000331"
     CDS             complement(1831..3033)
                     /gene="pncB"
                     /locus_tag="ACHKUI_000331"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6596602.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPKADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3299..5911
                     /gene="pepN"
                     /locus_tag="ACHKUI_000332"
     CDS             3299..5911
                     /gene="pepN"
                     /locus_tag="ACHKUI_000332"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6596603.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTAVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6106..6873)
                     /gene="ssuB"
                     /locus_tag="ACHKUI_000333"
     CDS             complement(6106..6873)
                     /gene="ssuB"
                     /locus_tag="ACHKUI_000333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6596604.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6870..7661)
                     /gene="ssuC"
                     /locus_tag="ACHKUI_000334"
     CDS             complement(6870..7661)
                     /gene="ssuC"
                     /locus_tag="ACHKUI_000334"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6596605.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7673..8818)
                     /gene="ssuD"
                     /locus_tag="ACHKUI_000335"
     CDS             complement(7673..8818)
                     /gene="ssuD"
                     /locus_tag="ACHKUI_000335"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6596606.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8815..9774)
                     /gene="ssuA"
                     /locus_tag="ACHKUI_000336"
     CDS             complement(8815..9774)
                     /gene="ssuA"
                     /locus_tag="ACHKUI_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6596607.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9767..10342)
                     /gene="ssuE"
                     /locus_tag="ACHKUI_000337"
     CDS             complement(9767..10342)
                     /gene="ssuE"
                     /locus_tag="ACHKUI_000337"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6596608.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10579..11589
                     /gene="pyrD"
                     /locus_tag="ACHKUI_000338"
     CDS             10579..11589
                     /gene="pyrD"
                     /locus_tag="ACHKUI_000338"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6596609.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGATLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11763..12305
                     /gene="zapC"
                     /locus_tag="ACHKUI_000339"
     CDS             11763..12305
                     /gene="zapC"
                     /locus_tag="ACHKUI_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6596610.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12302..13411)
                     /gene="ycbX"
                     /locus_tag="ACHKUI_000340"
                     /pseudo
     CDS             complement(12302..13411)
                     /gene="ycbX"
                     /locus_tag="ACHKUI_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
     gene            13509..15617
                     /gene="rlmKL"
                     /locus_tag="ACHKUI_000341"
     CDS             13509..15617
                     /gene="rlmKL"
                     /locus_tag="ACHKUI_000341"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6596611.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLVNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATILVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15629..17536
                     /gene="uup"
                     /locus_tag="ACHKUI_000342"
     CDS             15629..17536
                     /gene="uup"
                     /locus_tag="ACHKUI_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6596612.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDVIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQELLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17666..18919
                     /gene="pqiA"
                     /locus_tag="ACHKUI_000343"
     CDS             17666..18919
                     /gene="pqiA"
                     /locus_tag="ACHKUI_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6596613.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18924..20564
                     /gene="pqiB"
                     /locus_tag="ACHKUI_000344"
     CDS             18924..20564
                     /gene="pqiB"
                     /locus_tag="ACHKUI_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6596614.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKGKKDPEPKRAKQ"
     gene            20561..21124
                     /gene="pqiC"
                     /locus_tag="ACHKUI_000345"
     CDS             20561..21124
                     /gene="pqiC"
                     /locus_tag="ACHKUI_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6596615.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21380..21547
                     /gene="rmf"
                     /locus_tag="ACHKUI_000346"
     CDS             21380..21547
                     /gene="rmf"
                     /locus_tag="ACHKUI_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6596616.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21617..22135)
                     /gene="fabA"
                     /locus_tag="ACHKUI_000347"
     CDS             complement(21617..22135)
                     /gene="fabA"
                     /locus_tag="ACHKUI_000347"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6596617.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22204..23964)
                     /gene="ycbZ"
                     /locus_tag="ACHKUI_000348"
     CDS             complement(22204..23964)
                     /gene="ycbZ"
                     /locus_tag="ACHKUI_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6596618.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDDFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24150..24602
                     /gene="matP"
                     /locus_tag="ACHKUI_000349"
     CDS             24150..24602
                     /gene="matP"
                     /locus_tag="ACHKUI_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6596619.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24680..25720)
                     /gene="ompA"
                     /locus_tag="ACHKUI_000350"
     CDS             complement(24680..25720)
                     /gene="ompA"
                     /locus_tag="ACHKUI_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6596620.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNFDGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(26076..26585)
                     /gene="sulA"
                     /locus_tag="ACHKUI_000351"
     CDS             complement(26076..26585)
                     /gene="sulA"
                     /locus_tag="ACHKUI_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6596621.1"
                     /translation="MYTSGYAHRSSSFSSTASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26804..27433
                     /gene="sxy"
                     /locus_tag="ACHKUI_000352"
     CDS             26804..27433
                     /gene="sxy"
                     /locus_tag="ACHKUI_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6596622.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAPYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNIRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27396..29558)
                     /gene="yccS"
                     /locus_tag="ACHKUI_000353"
     CDS             complement(27396..29558)
                     /gene="yccS"
                     /locus_tag="ACHKUI_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6596623.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29568..30014)
                     /gene="yccF"
                     /locus_tag="ACHKUI_000354"
     CDS             complement(29568..30014)
                     /gene="yccF"
                     /locus_tag="ACHKUI_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6596624.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            30137..32191
                     /gene="helD"
                     /locus_tag="ACHKUI_000355"
     CDS             30137..32191
                     /gene="helD"
                     /locus_tag="ACHKUI_000355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6596625.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMHERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(32223..32681)
                     /gene="mgsA"
                     /locus_tag="ACHKUI_000356"
     CDS             complement(32223..32681)
                     /gene="mgsA"
                     /locus_tag="ACHKUI_000356"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6596626.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32777..33439)
                     /gene="yccT"
                     /locus_tag="ACHKUI_000357"
     CDS             complement(32777..33439)
                     /gene="yccT"
                     /locus_tag="ACHKUI_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6596627.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33612..34025
                     /gene="yccU"
                     /locus_tag="ACHKUI_000358"
     CDS             33612..34025
                     /gene="yccU"
                     /locus_tag="ACHKUI_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6596628.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(34070..34387)
                     /gene="hspQ"
                     /locus_tag="ACHKUI_000359"
     CDS             complement(34070..34387)
                     /gene="hspQ"
                     /locus_tag="ACHKUI_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6596629.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34445..35635)
                     /gene="rlmI"
                     /locus_tag="ACHKUI_000360"
     CDS             complement(34445..35635)
                     /gene="rlmI"
                     /locus_tag="ACHKUI_000360"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6596630.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35730..36008
                     /gene="yccX"
                     /locus_tag="ACHKUI_000361"
     CDS             35730..36008
                     /gene="yccX"
                     /locus_tag="ACHKUI_000361"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6596631.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(36005..36334)
                     /gene="tusE"
                     /locus_tag="ACHKUI_000362"
     CDS             complement(36005..36334)
                     /gene="tusE"
                     /locus_tag="ACHKUI_000362"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6596632.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36425..37084)
                     /gene="yccA"
                     /locus_tag="ACHKUI_000363"
     CDS             complement(36425..37084)
                     /gene="yccA"
                     /locus_tag="ACHKUI_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6596633.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFMLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37291..37378)
                     /locus_tag="ACHKUI_000364"
     tRNA            complement(37291..37378)
                     /locus_tag="ACHKUI_000364"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37342..37344),aa:Ser,seq:tga)
     gene            37805..38923
                     /gene="hyaA"
                     /locus_tag="ACHKUI_000365"
     CDS             37805..38923
                     /gene="hyaA"
                     /locus_tag="ACHKUI_000365"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6596634.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGELGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            38920..40713
                     /gene="hyaB"
                     /locus_tag="ACHKUI_000366"
     CDS             38920..40713
                     /gene="hyaB"
                     /locus_tag="ACHKUI_000366"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6596635.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            40732..41439
                     /gene="hyaC"
                     /locus_tag="ACHKUI_000367"
     CDS             40732..41439
                     /gene="hyaC"
                     /locus_tag="ACHKUI_000367"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6596636.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYGIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            41436..42023
                     /gene="hyaD"
                     /locus_tag="ACHKUI_000368"
     CDS             41436..42023
                     /gene="hyaD"
                     /locus_tag="ACHKUI_000368"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6596637.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAYIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
                     QPTEELRCLNYDCLSMENYEGVRLRQYRMRLEKQG"
     gene            42020..42418
                     /gene="hyaE"
                     /locus_tag="ACHKUI_000369"
     CDS             42020..42418
                     /gene="hyaE"
                     /locus_tag="ACHKUI_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6596638.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            42415..43272
                     /gene="hyaF"
                     /locus_tag="ACHKUI_000370"
     CDS             42415..43272
                     /gene="hyaF"
                     /locus_tag="ACHKUI_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6596639.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            43406..44950
                     /gene="appC"
                     /locus_tag="ACHKUI_000371"
     CDS             43406..44950
                     /gene="appC"
                     /locus_tag="ACHKUI_000371"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO6596640.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            44962..46098
                     /gene="appB"
                     /locus_tag="ACHKUI_000372"
     CDS             44962..46098
                     /gene="appB"
                     /locus_tag="ACHKUI_000372"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6596641.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            46111..46203
                     /gene="cbdX"
                     /locus_tag="ACHKUI_000373"
     CDS             46111..46203
                     /gene="cbdX"
                     /locus_tag="ACHKUI_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6596642.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            46283..47593
                     /gene="appA"
                     /locus_tag="ACHKUI_000374"
     CDS             46283..47593
                     /gene="appA"
                     /locus_tag="ACHKUI_000374"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6596643.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIANEARIPACALHQDK"
     gene            complement(47581..47793)
                     /gene="cspH"
                     /locus_tag="ACHKUI_000375"
     CDS             complement(47581..47793)
                     /gene="cspH"
                     /locus_tag="ACHKUI_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6596644.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            48079..48291
                     /gene="cspG"
                     /locus_tag="ACHKUI_000376"
     CDS             48079..48291
                     /gene="cspG"
                     /locus_tag="ACHKUI_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6596645.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            48302..48490
                     /gene="ymcF"
                     /locus_tag="ACHKUI_000377"
     CDS             48302..48490
                     /gene="ymcF"
                     /locus_tag="ACHKUI_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6596646.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            48465..48695
                     /gene="ymcE"
                     /locus_tag="ACHKUI_000378"
     CDS             48465..48695
                     /gene="ymcE"
                     /locus_tag="ACHKUI_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6596647.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVRIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            48685..48858
                     /gene="gnsA"
                     /locus_tag="ACHKUI_000379"
     CDS             48685..48858
                     /gene="gnsA"
                     /locus_tag="ACHKUI_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6596648.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(48907..49980)
                     /gene="yccM"
                     /locus_tag="ACHKUI_000380"
     CDS             complement(48907..49980)
                     /gene="yccM"
                     /locus_tag="ACHKUI_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6596649.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAIYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(50052..52795)
                     /gene="torS"
                     /locus_tag="ACHKUI_000381"
                     /pseudo
     CDS             complement(50052..52795)
                     /gene="torS"
                     /locus_tag="ACHKUI_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            52878..53906
                     /gene="torT"
                     /locus_tag="ACHKUI_000382"
     CDS             52878..53906
                     /gene="torT"
                     /locus_tag="ACHKUI_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6596650.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(53879..54571)
                     /gene="torR"
                     /locus_tag="ACHKUI_000383"
     CDS             complement(53879..54571)
                     /gene="torR"
                     /locus_tag="ACHKUI_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6596651.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            54701..55873
                     /gene="torC"
                     /locus_tag="ACHKUI_000384"
     CDS             54701..55873
                     /gene="torC"
                     /locus_tag="ACHKUI_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6596652.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            55873..58419
                     /gene="torA"
                     /locus_tag="ACHKUI_000385"
     CDS             55873..58419
                     /gene="torA"
                     /locus_tag="ACHKUI_000385"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6596653.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDISQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYGENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            58416..59015
                     /gene="torD"
                     /locus_tag="ACHKUI_000386"
     CDS             58416..59015
                     /gene="torD"
                     /locus_tag="ACHKUI_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6596654.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTATVNGLENSIATLTVRDDARLELAADFCGLFLMTDNQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LRQWLPEFVARCHQYDRFGFYAALSQLLQVLVECDNQKG"
     gene            complement(59372..59677)
                     /gene="cbpM"
                     /locus_tag="ACHKUI_000387"
     CDS             complement(59372..59677)
                     /gene="cbpM"
                     /locus_tag="ACHKUI_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6596655.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(59677..60597)
                     /gene="cbpA"
                     /locus_tag="ACHKUI_000388"
     CDS             complement(59677..60597)
                     /gene="cbpA"
                     /locus_tag="ACHKUI_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6596656.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            60750..60851
                     /locus_tag="ACHKUI_000389"
                     /pseudo
     CDS             60750..60851
                     /locus_tag="ACHKUI_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61132..62373
                     /gene="agp"
                     /locus_tag="ACHKUI_000390"
     CDS             61132..62373
                     /gene="agp"
                     /locus_tag="ACHKUI_000390"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6596657.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(62411..62638)
                     /gene="yccJ"
                     /locus_tag="ACHKUI_000391"
     CDS             complement(62411..62638)
                     /gene="yccJ"
                     /locus_tag="ACHKUI_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6596658.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(62659..63255)
                     /gene="wrbA"
                     /locus_tag="ACHKUI_000392"
     CDS             complement(62659..63255)
                     /gene="wrbA"
                     /locus_tag="ACHKUI_000392"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6596659.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            63628..63801
                     /gene="ymdF"
                     /locus_tag="ACHKUI_000393"
     CDS             63628..63801
                     /gene="ymdF"
                     /locus_tag="ACHKUI_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6596660.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(63884..65212)
                     /gene="rutG"
                     /locus_tag="ACHKUI_000394"
     CDS             complement(63884..65212)
                     /gene="rutG"
                     /locus_tag="ACHKUI_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6596661.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(65233..65727)
                     /gene="rutF"
                     /locus_tag="ACHKUI_000395"
     CDS             complement(65233..65727)
                     /gene="rutF"
                     /locus_tag="ACHKUI_000395"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6596662.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQESLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(65738..66328)
                     /gene="rutE"
                     /locus_tag="ACHKUI_000396"
     CDS             complement(65738..66328)
                     /gene="rutE"
                     /locus_tag="ACHKUI_000396"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6596663.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(66338..67138)
                     /gene="rutD"
                     /locus_tag="ACHKUI_000397"
     CDS             complement(66338..67138)
                     /gene="rutD"
                     /locus_tag="ACHKUI_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6596664.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(67146..67532)
                     /gene="rutC"
                     /locus_tag="ACHKUI_000398"
     CDS             complement(67146..67532)
                     /gene="rutC"
                     /locus_tag="ACHKUI_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6596665.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(67544..68236)
                     /gene="rutB"
                     /locus_tag="ACHKUI_000399"
     CDS             complement(67544..68236)
                     /gene="rutB"
                     /locus_tag="ACHKUI_000399"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6596666.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(68236..69327)
                     /gene="rutA"
                     /locus_tag="ACHKUI_000400"
     CDS             complement(68236..69327)
                     /gene="rutA"
                     /locus_tag="ACHKUI_000400"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6596667.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDNAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            69615..70253
                     /gene="rutR"
                     /locus_tag="ACHKUI_000401"
     CDS             69615..70253
                     /gene="rutR"
                     /locus_tag="ACHKUI_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6596668.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(70293..74255)
                     /gene="putA"
                     /locus_tag="ACHKUI_000402"
     CDS             complement(70293..74255)
                     /gene="putA"
                     /locus_tag="ACHKUI_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6596669.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDERVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            74310..74519
                     /locus_tag="ACHKUI_000403"
     CDS             74310..74519
                     /locus_tag="ACHKUI_000403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596670.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            74678..76186
                     /gene="putP"
                     /locus_tag="ACHKUI_000404"
     CDS             74678..76186
                     /gene="putP"
                     /locus_tag="ACHKUI_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6596671.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            76406..77236
                     /gene="efeU"
                     /locus_tag="ACHKUI_000405"
     CDS             76406..77236
                     /gene="efeU"
                     /locus_tag="ACHKUI_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6596672.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            77294..78421
                     /gene="efeO"
                     /locus_tag="ACHKUI_000406"
     CDS             77294..78421
                     /gene="efeO"
                     /locus_tag="ACHKUI_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6596673.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKSADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            78427..79698
                     /gene="efeB"
                     /locus_tag="ACHKUI_000407"
     CDS             78427..79698
                     /gene="efeB"
                     /locus_tag="ACHKUI_000407"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6596674.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMLHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            80325..81113
                     /gene="phoH"
                     /locus_tag="ACHKUI_000408"
     CDS             80325..81113
                     /gene="phoH"
                     /locus_tag="ACHKUI_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6596675.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(81163..81576)
                     /gene="pgaD"
                     /locus_tag="ACHKUI_000409"
     CDS             complement(81163..81576)
                     /gene="pgaD"
                     /locus_tag="ACHKUI_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO6596676.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(81578..82903)
                     /gene="pgaC"
                     /locus_tag="ACHKUI_000410"
     CDS             complement(81578..82903)
                     /gene="pgaC"
                     /locus_tag="ACHKUI_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO6596677.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDHMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(82896..84914)
                     /gene="pgaB"
                     /locus_tag="ACHKUI_000411"
     CDS             complement(82896..84914)
                     /gene="pgaB"
                     /locus_tag="ACHKUI_000411"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO6596678.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVAVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(84923..87346)
                     /gene="pgaA"
                     /locus_tag="ACHKUI_000412"
     CDS             complement(84923..87346)
                     /gene="pgaA"
                     /locus_tag="ACHKUI_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO6596679.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQTAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            complement(87353..>87540)
                     /locus_tag="ACHKUI_000413"
     CDS             complement(87353..>87540)
                     /locus_tag="ACHKUI_000413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596680.1"
                     /translation="WNGFSGEKRILYGQSAHHFFFSSSTIHVSLPRLITDYEMQKYLS
                     FQLPVIRYRERFWQYME"
BASE COUNT        21475 a        22083 c        23039 g        20943 t
ORIGIN      
        1 ttatcgacca ggcacaaaat cttctttcag acatccagaa tgccaaaaat ttgtatgaaa
       61 attgtgctat cagctaagaa aaaactaatc cgcaataaga atgcagatta ttttttgtgc
      121 gtaaatgttg cagcgtaata ttccgtaaca ggatgatcgt tatggcgggg ttgcaaatag
      181 attgcttgac ttaacaaaca gataacttga cagaaaggat aaaaaaagag ccagcgggcg
      241 ctggctcttg aagacgctaa attagaagct ggcgttacgc ggagtacgtg ggaacggaat
      301 aacatcacgt acgttttgca cgccagttac gtaagcaatc aggcgttcaa aaccaagacc
      361 gaaacctgaa tgcggaacag taccgtagcg acgcagatcg cgataccacc agtaatcttc
      421 tttgttcagg cccatttcaa gcatacgctc gtccagcacg tccagacgtt cttcacgctg
      481 ggagccacca atgatctcac cgatgcccgg agccagaacg tccatagccg caacggtttt
      541 accgtcttcg ttaaggcgca tatagaacgc tttaatatct ttcggatagt ttttaaccac
      601 taccggtgct ttaaagtgtt cttccgccag ataacgctca tgctcagaag agagatcgac
      661 gccccagtaa accgggtttt caaacttcct gccgcagttt tcgagaatgg tcactgcgtc
      721 ggtgtaatcc acctgcgcga aatcggcctc aatgaagcgt tccagacgtg aaacggcatc
      781 tttatctacg cgttcagcga agaatttcat gtcgtcagcg cgttcttcga gaaccgcttt
      841 gaagacatat ttcagcatgg cttcagccag acctgcgata tcgttcaggt tagcaaacgc
      901 cacttccggc tccagcatcc agaattccgc caggtgacgg ctggtgttgg agttttcagc
      961 acggaaagtc gggccgaagg tataaatttt ggacaatgcg caagcgtagg tttcgccgtt
     1021 caactggccc gatacggtca ggaaagactc tttaccaaag aagtctttgt cgaaatccac
     1081 tttgccctga tcgttacgcg gcaggttttc cagatccagc gttgaaacac ggaacatttc
     1141 gccagcacct tcggtatcag atgcggtaat cagtggcgtt gaaacccaga agaatccctg
     1201 ctcgttaaag aagcgatgca gcgcctgcgc cagcgtatgg cgaacgcgcg cgacggcacc
     1261 aatcaggttt gtgcgcggac gcaggtgagc gacttcacgc agatactcaa tgctgtggcg
     1321 ttttgccgcc atcgggtaag tgtctggatc ttcaacccaa ccagcaactt caaccttgct
     1381 ggcctgaatt tcaaattgtt gcccctggcc cggcgacgcc acgactttac ccgtcacaat
     1441 gaccgagcag ccggtggtca gacgcaagac gtcttcattg taattgggca gagaattatt
     1501 gatgacagcc tgtacaggat caaagcagga accgtcataa acggcgagga aggagatgcc
     1561 agcttttgaa tctcggcggg tacgtaccca tccgcgcacg gtgacttcgc tgtcaacggc
     1621 tacgcggccc tggagtacgt cggctacagg cacaacgctc ataatattct ctctgttaat
     1681 agtcggaaaa aataaacact tgtccacccg taaatggggg gatacctatg ttacctggca
     1741 tctgcaatca gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa
     1801 aaaagggagc cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc
     1861 gaacgctttg cgcagcgccc gaacaaacgc tttatcgtgg cagatagttt ttccagggct
     1921 gtcagaaagt ttcgccaccg gtttaccgtt acactctacc aacttaatga caatattcag
     1981 aggttttacc tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac
     2041 gcgggaagag aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa
     2101 aaccagcgtt ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc
     2161 accccattca accgggtcac cagagtcatg gcgcaggccc tgatagcgac tggcgaactc
     2221 gacaccgaaa tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag
     2281 ttggtcggga tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc
     2341 cgggctgatt tgctgatgcg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag
     2401 ggaaagccga cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag
     2461 acgtttcacg atggtttctt gtacttcgcg agaaaaacgg cggcgggtgc caaaatccat
     2521 cagatggaag cgcgacatat caagaccggc ggttaatgcc gagaagtcga ctaatttgtt
     2581 ttccagcgtg tcgagggctt gcgcaacgtc ggccttcggt gagcgatagc gatgtaccat
     2641 ttcactgata accgccagca aaggaacctc ccagaggatg acttcacgcc acgggccgct
     2701 taaacgaata tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg
     2761 gaactcgcgt agccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg
     2821 atattcatca tcctgcaggc gcaggtgctg catcgcctga acctgttcac gaatagcatc
     2881 ggcataaata cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc
     2941 gtaatagtga tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa
     3001 cgagtgcaga acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg
     3061 agcgtttagt acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta
     3121 ttgaaccccg atcacaccat atgccacctt tctggtcgat ggcataacgc ggtgtatgtt
     3181 ataaaaatgt agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga
     3241 tttacgcaac aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat
     3301 gactcaacag ccacaagcca aataccgtca cgattatcgt gcgccggatt accagattac
     3361 tgatattgac ttgacctttg acctggacgc gcaaaagacg gccgttaccg cggtcagcca
     3421 ggctgtccgt catggtgcat cagatgttcc gcttcgtctc aacggcgaag acctcaaact
     3481 ggtttctgtt tatattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact
     3541 ggtcatcagt aatttgccgg agcgttttac gcttaagatc gttaatgaaa ttagcccggc
     3601 ggcgaatact gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga
     3661 agccgaaggt ttccgccata ttacgtatta tctcgaccgc ccggatgtgc tggcgcgttt
     3721 taccaccaaa attattgccg ataaaaccaa atatcccttc ctgctttcca atggtaaccg
     3781 cgttgcgcaa ggtgaactgg aaaatggacg tcattgggta cagtggcagg acccgttccc
     3841 gaaaccgtgt tacctgtttg cgttggtggc aggcgacttt gatgtgctgc gcgacacttt
     3901 caccacgcgt tctggtcgcg aagtagcgct ggagctgtac gtcgatcgtg gcaaccttga
     3961 tcgcgcgccg tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt
     4021 cggcctggag tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg
     4081 cgcaatggag aataaggggc tgaatatctt taactccaaa tatgtgctgg cccgcaccga
     4141 caccgccacc gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca
     4201 taactggacc ggcaaccgtg tcacctgccg cgactggttc cagctcagtc tgaaagaagg
     4261 tttaaccgtc ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cggtaaaccg
     4321 tatcaataat gtgcgcacca tgcgcggatt gcagtttgca gaagacgcca gcccgatggc
     4381 gcacccaatt cgcccggata tggtcattga aatgaacaac ttctacaccc tgaccgttta
     4441 cgagaagggc gcggaagtga ttcgtatgat ccacactctg cttggcgaag aaaatttcca
     4501 gaaagggatg cagctctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgattt
     4561 tgtgcaggcg atggaagatg cgtcgaatgt cgatctttcc catttccgcc gttggtacag
     4621 ccagtccggc acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta
     4681 caccctgacc atcagccagc gcacgccagc tacaccggat caggcagaaa aacaaccact
     4741 gcatattccg tttgccatcg aactgtatga caacgaaggc aaagtgatcc cgttgcagaa
     4801 aggcggtcat ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt
     4861 tgataatgtc tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa
     4921 actggaatat aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga
     4981 tttctctcgc tgggacgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc
     5041 ccgtcatcag caagggcagc cgttgtctct gccggtgcat gtggctgacg ctttccgcgc
     5101 ggtgctgctc gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc
     5161 cgtcaatgaa atggcagaac tgttcgatat catcgacccg attgctattg ctgaagtacg
     5221 cgaagcactc actcgtactc tggcgactga actggcggat gagctactgg ctatttacaa
     5281 cgcgaattac cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg
     5341 taatgcctgc ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag
     5401 caagcaatac catgaagcaa acaatatgac cgatgcgctg gcggcgcttt ctgcggcggt
     5461 tgctgcacaa ctgccatgcc gtgacgcgct gatgcaggag tacgacgaca agtggcatca
     5521 ggatggtctg gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt
     5581 gctggagacg gtacgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg
     5641 cattcgttca ttgattggcg catttgcggg cagcaacccg gcagcgttcc atgccgaaga
     5701 tggcagcggt tatcagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca
     5761 ggtggcttca cgcctgattg agccgctgat tcgcctgaaa cgttacgatg ccaaacgtca
     5821 ggagaaaatg cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct
     5881 gtacgagaag atcaccaaag cactggcttg atacttttga gccaaaatgc ttgacgcgtc
     5941 ttatcaggcc tacaagtccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
     6001 ccgcatcagg cagtcgtgcc caactgcctg atgcgacgct tgacgcgtct tatcaggcct
     6061 acaatcccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgtcg
     6121 aatcggttgt tcagagtgcc cacgttgcat cacccgttgc aacacttccg cttccagctc
     6181 cgctaatcgc accgacccta accggcgcgg acgggggata tccaccgtca aatccagacc
     6241 aatttttccc tcttcaatta acaacacccg gtcagccatc gccaccgctt cgctcacatc
     6301 atgcgtcacc agcaaaacgg taaagccgtg ctcctgccag agtgacacaa tcaaatcctg
     6361 catctcgagt cgcgttaaag cgtccagcgc accgagtggt tcatcaagca acaataaacc
     6421 cggtcgatga atcagcgccc ttgccagcgc cacacgttgt ttctgcccgc cagaaagtgc
     6481 ggcaggccat tcccccgcgc gattctccag ccctaccgca gccagagcct gacgcgccgc
     6541 atcgcgccac tggcctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
     6601 cagacgcgcg tcctgaaaca tcattcgcgt atcatcctga atctcagcca gcggtgtggt
     6661 gcccgctaac acatcgccag cggttggcgt ttccagacct gccagcaggc gcagcagggt
     6721 acttttgcca ccgccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
     6781 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
     6841 tggtgtgccc tggttcagac gagcagtatt cataccgtgg cctccttcaa atgataagcc
     6901 gggttccagc gcaaccagag acgctctaac agctgcgcgc tgacgtcagc aagtttgccg
     6961 agcagggcgt aaagaataat ggcgactacc accacgtccg tttgcagaaa ctcccgcgca
     7021 ttcatcgcca gataaccaat gccggaattg gcagaaatag tttcggcaac gatcagcgtc
     7081 agccacatca ggcccaaagc aaaacgcaca ccgaccataa ttgagggcag ggcaccaggc
     7141 aggatcacat ggataaacag cggtatgccg gataatccat agctacgtgc catctccacc
     7201 agcccacggt cgatattacg gatgccgtgc caggtgttga tataaatggg gaatagcgta
     7261 ccgagcgcca ccagaaatat tttagcggat tcatcaatgc cgaaccacaa aatcaccagc
     7321 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagccgt
     7381 tctccccagc gtgacagccc gctaatcagc cccagaatta atcccagtga tccgccaatt
     7441 gaaaagccaa tcaacgcacg ccaggagctg atcgccagat gctgccacag ttcgccgctg
     7501 gcagagagcg tccagaacgc catcaccact ccttccggtg agggcaaaat acgcgtcgat
     7561 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
     7621 ggagcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
     7681 gcgcgacgtt acgggggata aaatcattcg ccaccgcttc gccttgtgga ttcagcggtt
     7741 gtggctgggg aatttccggg atggcgacat ccagatgtgg gaacagtaac tcgccgaccc
     7801 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa actgtcgatg ccaagtgcag
     7861 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gtcccggcac
     7921 cgccgcgcac taagccaaca cccgcccata aattggggct gatctccaga ttgtcgcgct
     7981 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtgcgg gcgaatgcgg
     8041 cctgtgcttt ggcgatagtt tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
     8101 acgcttcgtc gttggtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
     8161 gtccatgcgc ggcagctttc gcccgcacgt gttcgatttt ctcttttacc agttccggtg
     8221 gttcgcccca ggtaaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
     8281 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagtttgg
     8341 ctccgcgtac atgaatatgt ttgccgttga aatccacagt ctcgccaagc aacaaccgcc
     8401 gccagacctg ggtaaattct gccgaggctt cgtaacgctc gctatgatca aggaacacgc
     8461 cgtcgcctgc cagttcttgt ggatcgctgc ctgtgaccag gttaaacaac gcacgtccgt
     8521 tagagagacg gtcaagcgtg gcggcctgac gggcggcaac ggtaggtgag gttacgctgg
     8581 ggcgcagggc gacaagaaac ttcagccgtt gtgtcaccgg gatcatcgac gccgccacca
     8641 gccacgcatc ttcacacgat cgccccgtcg ggatcagcac accggtatag cccagacgat
     8701 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cggacgtgaa ccttcttccg
     8761 ttcccagata atgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
     8821 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
     8881 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacttcagc
     8941 gctcaacggt ttgatggtgg tgggaggacg atgatccaga tatgaggcaa taaccggtgc
     9001 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
     9061 cgcatcggcc tcactaaagg ttgtcagcac accctgaata aaagcgccgt ttttttctgc
     9121 atacggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
     9181 cacccgcacg ccgccctgta ataatgcagc ggagtagtag ggatcccaga tagcccaggc
     9241 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
     9301 aatatccgtg aatttaagcc cggcctggcg aagggctcgc agtaaaagat tgtgcgaact
     9361 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
     9421 gttttctgcc accagaatca cttcggcttt gggtttcggt ggctcgacgc ccacgtatac
     9481 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
     9541 gatactgcca acgtttaacg cctccagcat ctgtgggcca gcggggaact ccacccagga
     9601 gatttttgtt tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
     9661 aatactgcct ttttgatagc ctatacgtaa cgcctctggc gaagactccg cggcaacagc
     9721 aagtgtagag acgctaagta aacccgccag cgccagttta atgatcttac gcatgggcat
     9781 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaac gcctgccaga
     9841 aagtttctag cgcggtatca agacgagttt gcagatttgg cgtgaactgg ggtttgtgtt
     9901 gataatcaat gacttgcgag tcatcggcaa acacgccgtg caggatctcc tgggctttca
     9961 gcgtacttaa aactggctta agggcataat cgaccgccag cagatgggcc acggtgccgc
    10021 ctgtcgccag cggcagcacc actttgcctt gcaacgcacg ttctggcagc aggtcgagca
    10081 gggttttcag tgcaccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    10141 cggcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    10201 gcagatcttc cgggacgaag ttttgcaaat tccagtgata aacctctaca tccaggccat
    10261 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    10321 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttg ttttgttaac
    10381 attgataaca attcggtcag tctgacagag ggcgtccgcg agtcgaaatg atttatctga
    10441 ggaagaaaag ccaaaaaaag cataaaaaaa gcgcaataag aaaacgtttg ctatttattg
    10501 ccgcaggtca attccctttt ggtccgaact cgcacataat acgcccccgg tttgcacacc
    10561 gggaatccag gagagttcat gtactacccc ttcgttcgta aagccctttt ccagctcgat
    10621 ccagagcgcg ctcatgagtt tacttttcag caattacgcc gtattacagg aacgccgttt
    10681 gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta actgcatggg cctgacgttt
    10741 aaaaatccgc ttggtctggc agccggtctt gataaagacg gggagtgcat tgacgcgtta
    10801 ggcgcgatgg gatttggatc catcgagatc ggtaccgtca cgccacgtcc ccagccaggt
    10861 aatgacaagc cgcgtctctt tcgtctggta gatgccgaag gtttgatcaa ccgtatgggc
    10921 tttaataatc ttggcgttga taacctcgta gagaacgtaa aaaaagccca ttatgacggc
    10981 gttctgggta ttaacatcgg caaaaataaa gatacgccgg tggagcaggg taaagatgac
    11041 tatctgattt gtatggaaaa aatctatgcc tatgcgggat atatcgccat caatatttca
    11101 tcgccaaata ccccaggatt acgcacactg caatatggtg aagcgctgga tgatctctta
    11161 actgcgatta aaaataaaca aaatgatttg caagtgatgc accataaata tgtgccgatc
    11221 gcagtgaaga tcgcgccgga tctttctgaa gaagaattga tccaggttgc cgatagttta
    11281 gttcgccata atattgatgg cgttattgca accaatacca cactcgatcg ttctctggtt
    11341 cagggaatga aaaattgtga tcaaaccggt ggcttaagtg gtcgtccgct tcagttaaaa
    11401 agcaccgaaa ttattcgccg cttgtcacag gaattaaacg gacgcttgcc gattattggt
    11461 gttggcggca ttgactcggt tatcgctgcg cgtgaaaaga ttgctgcggg tgccacactg
    11521 gtgcaaattt attctggttt tatttttaaa ggcccgccgc tgattaaaga aatcgttacc
    11581 catatctaat tatttcttca aattcctctt tataagataa tcagggcttt attttcagcc
    11641 ctggttgttt tatattcatc gctgttgctt atttagacat tttgtacttt tattgttgag
    11701 gttattaagc gaagcgacaa tggattgtgt cgctgcggtt ttataaggtt ggggagtggc
    11761 agatgcgaat taaaccagac gataactggc gttggtatta cgatgaagag cacgatcgaa
    11821 tgatgctcga tttagccaat ggtatgctat ttcgctcacg ttttgcgcgc aaaatgttga
    11881 ctccagatgc cttttctccc gcaggttttt gcgttgatga cgccgcgctc tatttctctt
    11941 ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca aaaagccgaa ctggtgctga
    12001 atgcactggt agcaattcgt tatctcaaac cgcaaatgcc gaaaagctgg cattttgtat
    12061 ctcatggtga aatgtggatg cccatgccgg gcgatgcggc ctacgtttgg ttgagcgaca
    12121 ctcatgagca agtgaattta ctggtcgttg aatctggcga gaatgccgcg ctatgtctac
    12181 tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca gttgggcgac gccattaaaa
    12241 tcatgaacga caggctgaaa ccgcaggtta atgttgacag cttcagcctc gaacaggcag
    12301 tctaacgcgc cagcttaagc gcagtcttcg gtacacaact acagcaaaga atagtgccat
    12361 catcgcctat tgctgatttt tttagcggcg tgacttcgcc ttctaaaagc tgaacacggc
    12421 aacttccaca aatgcccgcg cggcaagaat aagggatacg aattccctga ttttctaatt
    12481 gctccagcag cacctgttgg ttatttccac gaaatgcctg tccctgccag tcaatatcta
    12541 cgtttgcgtc cggttgttgc gtgatgttgg cagtatcatc agcggcacct gcgccgtaaa
    12601 ttttagccgg agccgttgcc agaatttcca cctcgtcgcc aacgcgaatc acgccgctat
    12661 tacgggcaat taaattctga ccaaaatcga catcgccgtt atcctgggca gtacggaaag
    12721 attgcagcgt ttttaatggt tcgcctgccg gatgtttttg ccctttttct gggctgacgg
    12781 tggtgaaaat acagcggcta caaggcttaa ccacatcaaa cacgacatca ccaatgcgaa
    12841 tcactttcca gctatcttct tcctaggccg aagcaccgga aaccaccaga ttggggcgga
    12901 attgctccat ttttacactg gccggacaac gttgttggag atcacgtaac gaggcctcgt
    12961 tagcaagaag gtaaggatag ccatcagcaa atgacagggg tacagtgttg tggcgtttca
    13021 cgcgccgggt catttgtggc cccacccagc gtaattgcac ttcgcgggag aaaaatccac
    13081 ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt aaaatgtgtg ccccaaactt
    13141 cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac atatgcacta ctgccatctg
    13201 gtgcggtgag atgcaagcca tcatgcacgg gtgaaggagt aaaccgtacc attagcggaa
    13261 actggcgagc ggtaataaac gtaccgtcag gttccgtgat cataaagatg cgatcgaagg
    13321 ccagaccact gacatctgcc agggtatgtg taagaccaat gccgcgcatc gatttaacag
    13381 gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc ctcaaatgaa aataagccct
    13441 caactttatg acatgacgcg cttattagct ataatgcgca acaattttct tagagtaaaa
    13501 gtgacaatat gaattctctg tttgccagta cggcccgtgg gctggaagag ctgttaaaaa
    13561 ctgaactgga aaacctgggg gccgttgaat gccaggtggt tcagggtggg gtccatttca
    13621 agggcgacac acggcttgtt taccagagcc tgatgtggag ccgcctggcc tcgcgtatta
    13681 tgttgccgct gggcgagtgt aaggtttaca gtgatttaga cctctatctc ggcgttcagg
    13741 cgatcaactg gacagagatg tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt
    13801 tgaatgacac catacgcaac agtcagtacg gtgcgatgaa agtgaaagac gcgatcgtcg
    13861 atgctttcac gcggaaaaat ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc
    13921 gcgttaacgt ctggctgcat aaagaaaccg ccagtatcgc ccttgatctc agtggtgatg
    13981 gtttacatct gcgtggctat cgcgatcgtg ctggcattgc gccgatcaaa gaaaccctgg
    14041 cagccgctat tgtgatgcga tccggctggc agccaggaac accgctgctc gatccaatgt
    14101 gtggttcagg tacgttgctg attgaagcag cgatgctggc gaccgaccgc gcaccgggct
    14161 tgcaccgtgg gcgttggggc tttagcggct ggacgcagca tgatgaagct atctggcagg
    14221 aagtgaaagc ggaagcgcaa actcgtgccc gtaaaggcct ggcggagtat agctcccatt
    14281 tctacggttc ggacagtgac gcacgggtga ttcaacgtgc gcgcactaac gcccgtcttg
    14341 cggggattgg tgaactgatc acctttgagg tgaacgatgt cgcgcaactg gtcaatccgc
    14401 tgccgaaagg gccgtacggt acagtgttga gcaacccgcc atacggtgaa cgtctggaca
    14461 gcgaaccggc gctgattgcg ctgcatagtc tgctgggccg aatcatgaaa aaccagttcg
    14521 gtggctggaa tctctctttg tttagtgcct cgccggatct gctaagctgc ctgcagctgc
    14581 gtgcagacaa acagtacaag gcgaaaaacg gcccgctgga ctgcgtacag aaaaactacc
    14641 atgttgccga aagcacacca gacagcaaac cggtaatggc agcggaagac tacgccaacc
    14701 gtctgcgtaa gaacctcaaa aaattcgaga agtgggctcg ccaggaagga attgaatgtt
    14761 accgcctgta tgacgccgat ctgccggaat ataacgttgc cgttgaccgt tatgccgact
    14821 gggtggtggt gcaggagtat gcgccgccaa aaactattga tgcgcacaaa gcgcgtcagc
    14881 gtctgttcga tattatcgct gcaaccattt tggtactggg gattgcgcca aacaaactgg
    14941 tgctaaaaac ccgtgaacgc cagaagggca aaaatcaata ccagaaactg ggcgagaagg
    15001 gcgagtttct ggaagttacc gaatataacg ctcacttgtg ggtgaacctg acggattacc
    15061 tcgataccgg tctgttcctc gatcaccgta tcgcccgtcg tatgctcggt cagatgagca
    15121 aaggcaaaga tttcctcaac ctgttctctt ataccggcag cgccaccgtg cacgcgggat
    15181 taggcggtgc acgcagcact accaccgtgg atatgtcgcg tacttatctg gagtgggcag
    15241 aacgcaacct gcgtctgaat ggcctaaccg ggcgtgcgca tcgcctgatt caggccgatt
    15301 gcctggcgtg gctgcgtgag gctaatgaac agttcgatct gatatttatc gatccaccaa
    15361 ccttctctaa ctcgaaacga atggaagatg cgtttgatgt tcagcgcgat catctggcgc
    15421 tgatgaaaga tttgaaacgt ctgctgcgtg ctggtgggac gatcatgttc tcgaacaaca
    15481 aacgtggctt ccgtatggat cttgacggac tggcaaaact gggactgaaa gcacaagaaa
    15541 ttacgcaaaa aacgctctcc caggatttcg cccgtaaccg ccagatccac aactgctggc
    15601 tgattaccgc agcctgaaag gaatagtaat gtcattaatc agtatgcatg gcgcatggct
    15661 gtcgttcagc gacgcgccgc ttctcgataa cgcagaactg catatcgaag ataacgaacg
    15721 tgtttgtctg gtggggcgta acggcgcagg caaatcgacg ttaatgaaga tcctcaaccg
    15781 tgaacagggg ctggatgacg gtcgcattat ttatgagcaa gatgtgattg tagctcgtct
    15841 gcaacaggat ccgccgcgta acgttgaggg tagcgtttat gatttcgttg ccgaaggtat
    15901 tgaagaacaa gcggaatacc tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga
    15961 cccgagcgag aaaaatctca acgaactggc gaaggttcag gaactgctgg atcaccacaa
    16021 cctgtggcag ctggaaaacc gtatcaacga agtgctggcg caactggggt tagatcctaa
    16081 tgttgcgctg tcgtcgcttt ccggcggttg gttgcgtaaa gcggcattag gacgcgcgct
    16141 ggtgagcaat ccgcgcgtat tgctgctcga cgaaccgact aaccacctgg atatcgaaac
    16201 catcgactgg ctggaaggat tccttaaaac cttcaatggg acgattattt tcatctccca
    16261 cgaccgttcg tttatccgca atatggcgac gcgtattgtt gatctcgatc gcggcaaact
    16321 tgtgacctat ccagggaatt acgaccagta cctgctggaa aaagaagaag ccctgcgcgt
    16381 ggaagaacta caaaatgccg agttcgatcg caaactggcg caggaagaag tgtggatccg
    16441 tcaggggatc aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcaat
    16501 gcgtcgcgag cgtggcgaac gtcgcgaagt gatgggtacc gcaaaaatgc aggtggaaga
    16561 agccagccgc tccggtaaga tcgttttcga aatggaagac gtttgctacc aggttgacgg
    16621 taagcaactg gtgaaagatt tttctgccca ggttctacgc ggcgacaaaa ttgccctgat
    16681 tggtccgaat gggtgcggca aaaccacgct gctgaaacta atgctcggtc agcttcaagc
    16741 agacagcggg cgtattcatg ttggcaccaa actggaagtg gcttatttcg atcagcaccg
    16801 cgcggaactg gatcccgata aaacggtgat ggataacctt gctgaaggta agcaagaggt
    16861 gatggttaac ggcaagccac gccacgtatt gggctatttg caggacttcc tgttccatcc
    16921 aaaacgggcg atgacgccgg tacgtgcgct ttctggcggt gagcgtaacc gcttgttgtt
    16981 ggcgcgtttg ttcctcaaac caagcaactt attgattctt gacgaaccga ccaacgatct
    17041 tgatgtcgaa acgctggaac tgctggaaga actgatcgac agctatcagg gcacggtatt
    17101 gctggttagc cacgatcgtc agtttgtcga taacaccgtt acagaatgct ggatcttcga
    17161 aggcggcggt aaaattggtc gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga
    17221 acagtatgtg gcgctcaaac agcctgcggt gaaaaaaagc gaagaagccg ctgcgccaaa
    17281 agcagaaact gtaaaacgca gcagtagcaa actaagctat aaattgcagc gcgaactgga
    17341 gcagctaccg caattgcttg aagatctgga ggcgaagctg gaagctttac agacgcaagt
    17401 ggcggatgct tccttcttca gtcagcccca tgagcagaca caaaaagtgc tcgccgatat
    17461 ggctgctgca gagcaggagc tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt
    17521 aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag agacagctga caacggtaaa
    17581 attgcctgat gaactgggtt tatcaggcat gaaaaccaac actttgttag tcatctgcaa
    17641 aatagcgcat cattaaggag taccaatgtg cgaacatcat catgccgcga agcacatcct
    17701 gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt gagcatggtc agaaagcggc
    17761 atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat gcccctcggc agcgtcccac
    17821 cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa
    17881 tatgaacgtt gcaggagtta ccagtgaaat tacattactg gaaattcccg gcgtgctttt
    17941 ttctgaggac tacgccagcc tcggcacctt tttcctgttg tttgtgcaac tggttcccgc
    18001 gttttgtctg ataaccattc tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa
    18061 agagcaactg gcacgggtgc tttttcaact caaaacctgg ggaatggcgg agattttcct
    18121 cgcgggtgtg ctggtcagtt tcgttaaact gatggcttac ggcagcattg gggtaggcag
    18181 cagctttctc ccctggtgtt tattttgtgt cctgcaactg cgcgcttttc agtgcgttga
    18241 tcgtcgctgg ttatgggacg acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc
    18301 aggtgtcacg gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc
    18361 tgctgatgaa cccgtgtgcc cgcgttgtag taccaaaggg tacgttcggc gtagaaacag
    18421 cctgcagtgg acactcgcgc tgcttgtaac gtccatcatg ctgtatcttc cggctaatat
    18481 tttgcccatc atggtgacgg atttattagg ctcgaagatg ccgtcgacga ttctcgctgg
    18541 ggtcattctg ttatggagcg aaggctctta tcccgtcgct gcggtgattt ttctggccag
    18601 tattatggtg ccaacgttaa agatgatcgc cattgcgtgg ctgtgttggg atgccaaagg
    18661 acacggcaag cgcgacagtg aaagaatgca tttgatttat gaagttgttg agtttgtagg
    18721 ccgctggtcg atgattgacg ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg
    18781 aggtttaatg agtatttatc cggcaatggg tgcattaatg tttgctttag tcgtcataat
    18841 gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc
    18901 agagcatgag gagtcctgac agtatggaat ctaataatgg ggaagccaaa atccagaaag
    18961 tgaagaactg gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt ggggcctggg
    19021 ttctttttta tcattacagc catcagggac cggaagtgac cctgatcacc gcgaatgcgg
    19081 aaggaattga aggtggcaaa accaccatta aaagccgtag cgttgacgtc ggcgtggttg
    19141 aaagcgccac actggctgat gatttgacgc acgttgaaat caaagcgcgg ctgaattccg
    19201 gtatggaaaa attgctgcat aaagataccg tcttttgggt ggtgaaaccg cagattggtc
    19261 gagaagggat tagcggcctg ggaacgctac tgtctggggt ttatatcgaa ctgcagccag
    19321 gcgcgaaagg cagcaaaatg gataaatacg atttgttgga ctcgccaccg ttggccccgc
    19381 ctgatgcgaa aggtatccgt gtgcttctcg atagcaaaaa agccgggcag ctctcgccag
    19441 gagatccggt gctgttccgt ggctatcggg taggttcggt tgaaaccagc actttcgata
    19501 cgcaaaaacg caatatcagc tatcaactgt tcatcaatgc accttatgac cgactggtga
    19561 ccagcaatgt tcgtttctgg aaagatagtg gcattgcggt tgatctgacg tcagcgggaa
    19621 tgcgtgtgga gatgggctca ttgacaacgc tgcttagtgg cggtgtcagc tttgatgtgc
    19681 cggaaggtct ggatttaggg cagccagtgg caccgaaaac agctttcgtt ttgtatgatg
    19741 atcagaagag cattcaggat tcgttgtaca ccgatcacat tgattatctg atgttcttta
    19801 aagattcggt acgcggtctg caaccgggag ctccggtaga attccggggt attcgcctgg
    19861 gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg tcagacattt aacgatgatt
    19921 accgtattcc ggtactgatt cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa
    19981 atgcggatgt tgttgagcac cttggcgaat tgttgaaacg tggtttacgc ggatcgctga
    20041 aaaccggaaa cctggtcact ggcgcactgt atgttgatct cgatttctat ccaaatacgc
    20101 ctgcaataac cggtattcgt gaatttaatg gttatcagat tatcccgacc gtcagcggcg
    20161 gcctggcgca aatccagcaa cgactgatgg aagcgttgga taagatcaac aaactgccac
    20221 tgaatccgat gattgaacag gcaaccagta cgctttctga aagtcagcgc acaatgaaaa
    20281 acctgcaaac gacgctggat agcatgaaca agatccttgc cagccagtcg atgcagcagt
    20341 taccgacgga tatgcagtca acgttgcgtg aattgaatcg cagcatgcag ggcttccagc
    20401 ctggttccgc agcctacaac aagatggtgg cggatatgca gcgccttgat caggtgttgc
    20461 gagaactgca accggtgctg aaaacgctca acgagaagag taacgcgctg gtatttgaag
    20521 cgaagggcaa aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac
    20581 gattgcggca ctgtggctgg ccggatgcag ctccggcgaa attaataaaa actattacca
    20641 gttacctgtg gtgcagagcg gtacacaaag caccgccagc cagggtaatc gcctgttatg
    20701 ggttgagcag gtcgctgttc ctgactatct ggcggggaat ggtgtggttt atcaaaccag
    20761 tgatgtgaag tatgtgattg ccaacaacaa cctgtgggcc agcccgttgg atcaacagtt
    20821 gcgcaacacc ctggttgcca acctgagtac gcaactaccc ggctgggtgg ttgcctccca
    20881 gcctctggga agcgcccagg acacgctcaa tgttaccgtc acggagttta acggtcgcta
    20941 tgatggtaag gtcattgtca gtggtgagtg gctgttgaac caccagggac aactgatcaa
    21001 acgtccgttc cgtctggaag gagtgcaaac tcaggatggt tacgatgaga tggttaaagt
    21061 gctggccagt gtctggagtc aggaagccgc ttctattgcg caagagataa agcgtctacc
    21121 ttaatcataa agatttgtaa atataaccgt ctccggtatg ttgcctgagg cggttttttt
    21181 gtttctaacg tgcggaaaaa gttgttcctc ttcacatttt ttgtacaacc gacatgcccg
    21241 tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc
    21301 ggtattgctt aactgtgatt gcatatttag taatcactgt tttcttttcc accagaaacc
    21361 agtatgaggg aaacgaggca tgaagagaca aaaacgagat cgcctggaac gggcacatca
    21421 acgtggttat caggccggca tcgccggacg ctcaaaagaa atgtgtccct atcagacgct
    21481 gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc atggcggaca gggtagtaat
    21541 ggcctgattc tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg cctttatata
    21601 ctctgtctga ttaaaatcag aatgcagacg tatcctggaa cagacctact ttcaggtcgt
    21661 tggcggtata gatcaaacga ccatcaacca gcacttcacc atcagccagg cccataatca
    21721 gacgacggtt aactatgcgt ttaaagtgaa tacgataggt cacttttttc gccgtcggca
    21781 gcacctggcc tgtgaatttc acttcaccta cgcctagcgc gcgaccttta ccttcgccgc
    21841 ccagccagcc gaggtagaac cctaccagct gccacattgc gtccaggccc aggcatcccg
    21901 gcataaccgg atcgccgata aagtggcatc cgaagaacca cagatccgga ttgatatcca
    21961 gctctgcttc aacataccct ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac
    22021 ggtccatcat cagcatgttt ggtgctggca attgcgggcc tttagcacca aacagttcac
    22081 cgcgaccaga ggcaagaagg tcttcttttg tataggattc gcgtttatct accatgttct
    22141 ctgtaagcct tattttattg aagcacgcag gatagctaac acgtgtacgc tgaacaagtc
    22201 cgatcagttc ggaataaacc agttcagcca acgtaatggc catggaaaac ggtgacgtcc
    22261 ttcctgttgc gatgcttgtg cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc
    22321 ttcgccatcc cacaccagat ttaataataa cggcagtgcg tcagtcacat cgtctactgc
    22381 ccagatggtg aatttgtctt cttctaccgc tttcaccagt tcactgtgaa gacttaaatg
    22441 gcgaacgtta gctgtgggga taatgacacc ttgtttcccc gttaactcac gttgctggca
    22501 aatagcaaag aagccttcga ttttctcatt taaaccaccg actggctggg cgcgaccgaa
    22561 ctgatcgact gaacctgtga tagcgatact ctgattcacc ggcacatcgg cgagggcgct
    22621 tatcagggcg cagagttcag ccatcgaggc actatcgcca tccacttcac tgtatgactg
    22681 ctcaaatgtc agcgatgctg agaaggggat ctgttgctca agctgtagtt ccgacatcag
    22741 gaacgcttgc atgatcatca tccctttcgc atggatattg ccgccaagct ccgctttgcg
    22801 ttcgatgtcg gtgaattcac catcgccaat atgcacaacg cagctaatgc gagaaggttc
    22861 gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa agggcgttaa tttgcccgat
    22921 gcgttcgcct tcggtttcta tcaggatttg ctcctgaagg atctcatcct gcatgcgttc
    22981 agcgaggaaa ccttcgcgcc attcacgctg ctgtagcatt aagtttagct gctcgccgga
    23041 gaaggtgtcg ccatcacaca gggaggcgac ctcttgacac tggcggagga tccactgcgg
    23101 gctaagcgga agtgtttctt gttcaccagt gtagcgtgct gcttcgcgga taagtaccgg
    23161 ccaggcatcc gctcccggtg ctggtaagtg attatgtctg gcggtaaatg tcacccagcg
    23221 acaccactgg cttactgact ccgcatcgac aatctgcaga gtatcttcaa attcgctata
    23281 aatagcctgc tctgaaagct ctggctccat ctcctggaaa tcagccaatg attcgcgttc
    23341 gcctaccaga atgactttca gcttcaatgg catcgaaggc acagagacgg ggagagggcg
    23401 cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt tcagccgcat
    23461 ccacagcaga ggttgcgcca gcagtgtacg caaagagatg atgagaatac cgccatttgc
    23521 ctgatgcacc agaccaggct gcagggtaat atcgccatta aactgacgca ggcagccaaa
    23581 gagttgctct gcttctaccc agtcggcagc gacaacttgc gttaaagtcg caaaatcatc
    23641 atctgcactc actgcgtgac gtaagcggat ggtgtggccg gaaacctcat agtgaccgcc
    23701 caccagttgg cctgcatcgc tttgtagcgt acgcgcggca tcagcaataa gattcagata
    23761 ctcagactct tccggggcct tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa
    23821 ctgctccagc gcaaattgca gtcgcggttg agtatcactg aataaaggat cgttttcgtc
    23881 aatcaaatgt ggctgagcaa atatttcctg atagctatcg gtatcaggaa ccaggtcacg
    23941 ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag tctgttgtca aagccgcgat
    24001 tataccgtaa ccggcactgc agcacacgta gaaagcaacg acaatactcc tggcatgggc
    24061 gttaaagctc acaggatgga gattcttttc ttcactggcc taaaaagctg atattctgta
    24121 aagagttaca cggtaacatt gagatcgcta tgaaatatca acaacttgaa aatcttgaaa
    24181 gcggttggaa atggaagtat ctggtgaaga agcaccgcga aggggagtta atcacccgtt
    24241 acatagaagc cagtgccgcc caggaagccg tcgatgagtt gctctcgctg gaaaatgaac
    24301 ccgtgctggt aaatggttgg attgacaagc atatgaatcc ggaactggtc aatcgcatga
    24361 agcagaccat tcgggccaga cggaagcgcc attttaatgc agaacaccag catacgcgca
    24421 aaaaatcgat cgatctggaa tttatcgtct ggcaacgtct ggctggcctt gcgcagcgtc
    24481 gcggtaaaac gctttctgaa acgattgttc agctgattga agatgcggaa aacaaagaga
    24541 aatacgcgaa taaaatgtct tctttgaagc aggatctgca ggcattgctg ggtaaggaat
    24601 aactgtcaaa agtcagttca atttactaaa ggcgaaaaaa aaaccccgca gctgcggggt
    24661 ttttctacca gacgagaact taagcctgcg gctgagttac aacgtctttg atgcctttaa
    24721 cttcgatctc tacgcgacga tccggagcca ggcaatcgat cagtgcagca cgctgtttca
    24781 cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagattt
    24841 tgtctgccgg gataccttta gagatcaggt aatcaacaac ggactgagca cgacgctcgg
    24901 acagagcctg gttataagcg tcagaaccga tgcggtcagt gtaacccaga acaactacgg
    24961 aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac
    25021 cttccggttt cagggttgct ttgttgaagg tgaacagaac gtcagacttc agagtgaagt
    25081 gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga gctgcttcgc
    25141 cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga cgagtaccga
    25201 tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag
    25261 gagtgatcgc atactcaaca ccgccagcga agaccggaga aacgccggta tcgtggtttt
    25321 taccatcaaa gttggattta gtgtctgcac gccataccat accacccaga cgagtgtata
    25381 cgtccagatc gtcagtgatt gggtaaccca gtttagcggt cagctgaacg ccctgagctt
    25441 tatatgcacc gttttcaacg ctgcctttgt atggcatacg acctaaccag tcataaccca
    25501 tttcaaagcc aacatacggg ttaacctggt aaccaccaaa agcaccagcg cccagttggt
    25561 tttcatgggt tggaccattg ttgttgatga aaccagtgtc atggtactgg gaccagccca
    25621 gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac
    25681 cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc tcgttatcat
    25741 ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc accggggatc
    25801 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    25861 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    25921 cttgtataag gtacgtttaa tcttttttgt cagcgacaat ttacagaaga aaatcgtgga
    25981 aaccgcttca gacaagcctc cgcaaggaaa attagtcacg actgaaagca ttggctgggc
    26041 gataaaaaaa gttccaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt
    26101 tttagcccgg aaagttgtct cgttgcgtga gaggatgcgc ttaccggacg cataataaac
    26161 cccatagcgt taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa
    26221 tcatctgcca accaaccgat caccacgctg taattgcccg tgcgtaaagc gcgaaccatt
    26281 gactccacgg tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc
    26341 ccagatgcct gaacccattc gcgactcagt ttttgttgcg gtgttaacca gagttgccag
    26401 cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc
    26461 tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca
    26521 cgcgcaattt tacttgctgt ggatgagaac gacgaagaac gatgtgcata gcctgaagtg
    26581 tacataatca atccagcccc tgtgagttac tgtatggatg tacagtacat ccagtgacga
    26641 caaagatcaa ctctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa
    26701 cataaattaa tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt
    26761 aatgtgaatc attcttttat gttatgattt taaaaggaac tttatgaaaa gcctctccta
    26821 taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc
    26881 attgtttggt agttacagcc taaccgttga cgacacggtg tttgcgatgg tttctgatgg
    26941 tgagttgtat cttcgggctt gtgagcaaag tgcaccgtac tgtgtaaaac atccgcctgt
    27001 ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga
    27061 aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc
    27121 gctgaaagag aaaagcacgc gcaatattcg ggaaagactg aaagatttgc ccaatatgtc
    27181 ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat
    27241 acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa
    27301 gattctgttt atgcttgaag gggccattat cggcattcat gaagctgcgc tcccggtggc
    27361 acgccgccag gagcttgcag aatgggctga ctctcttacg ccgaaacagg agtttcctgc
    27421 ggaacttgag taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac
    27481 ccaacttgtt gtacgaccag aggttctttg ctgtctgccc gtggttcaag ttgctgcatc
    27541 cgctgtttca ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt
    27601 agcgcgtcat caacatagca aacggcgtca tcaagaaacg ccagaatttc aggattagtt
    27661 aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac
    27721 agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt
    27781 gataccaccg acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac
    27841 gccagacggt tatcacgccc ctgatggtat tgttccagta tggcatctag ataccggcag
    27901 ttggcctctg tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag
    27961 atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt
    28021 aacgccactt caaaaccttc acccagcaag ttgaaacaca gtagcaccaa aagtgtgatg
    28081 aacatcgttg catgggcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata
    28141 accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg aataccaatt
    28201 gcgataccta ccagcgtacc aataatcctt aacttcagcc ggtggcgcgt ggcgttatag
    28261 tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt
    28321 atctgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag
    28381 agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc
    28441 gggctgtcat cagcgagctc attttcgtca ttattatggg gcagtgcctg ggcctgttct
    28501 gattcaattg ttgccagttg ggcatcaatg gcacgtaaat tgttcagtaa aaatcccagt
    28561 gttttgagta aatcagcagg agcgccgtta tcgcgcatcc gctccagcgc agcatcaata
    28621 tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag gctgacgcag caaaatacag
    28681 cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc
    28741 acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatatt gaatatgaga agagctggca
    28801 cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagtg tgcgacgcgt tcctcgttga
    28861 ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc
    28921 agctgaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca
    28981 ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc
    29041 aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg
    29101 taccagacgg caccggccag aagatacatt ggctgctgat accagtgctc atacagtgat
    29161 gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt
    29221 tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat
    29281 agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc
    29341 gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct
    29401 gccaccatcc caagcgtcag cggaatcgtc agttttacat cacccagcca ccacggaaac
    29461 gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag
    29521 gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta ttggaaacga
    29581 cgacgagcat tggcttcgcg cgcagcttgc gctgtttcta ccgataccac gcgacgaccg
    29641 accggccata gtgcaatagc ggcaatttta aagttcgcaa taccgacagg aatgccaatg
    29701 atggaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag
    29761 aaaatcagcc agaaaatatt caataccgta ccgccagtat ttagcagcac atttttgcca
    29821 gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag agacagtttg
    29881 gtgatctccc agcaggatcg tgtcagtggt aaggtaaaaa tcagcacaat actgaccaga
    29941 gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc
    30001 aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa
    30061 cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt
    30121 gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca
    30181 cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga
    30241 ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg
    30301 cgaactggag tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca ccgaatgggg
    30361 cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat
    30421 gagcgaaatt gcgtctggcg ttttacgtca gcaactggat ttgattgcca cgcgcaccgg
    30481 agaaaataaa tggctgacgc gtgagcaaac ctctggcgtg cagcaacaaa tccgccaggc
    30541 tttgtcggcg ttaccattgc cggttaaccg actggaagaa ttcgataact gccgtgaggc
    30601 gtggcgtaaa tgtcaggcct ggttgaagga cattgaaggc gctcggttgc agcataatca
    30661 ggcgtatacc gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc
    30721 accgctgaat ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct
    30781 ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgctggct ggttactggc
    30841 gcgtggtgaa gcgtctcctg agcaaatttt attgctggcg tttggtcgca aagccgctga
    30901 agagatgcac gagcgtattc gagaacgact acataccgaa gacattaccg cacgcacgtt
    30961 tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa
    31021 actggaaaat gataccgctg cccgtcatga actttttatt gctgagtggc gcaagcaatg
    31081 cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg
    31141 gtcagtgcca gaaggtaatt tctgggatga tgaaaaatta cagcgtcgtc ttgcctctcg
    31201 cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc
    31261 cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt
    31321 attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat
    31381 tcaccaggcg attgtgattc tggagaaagg tcgctttatc agcccgtgga agcatattct
    31441 ggttgatgaa tttcaggata tctcgccgca gcgtgcagcg ttgttagcgg cattacgcaa
    31501 gcaaaatagt caaacgacgt tgttcgctgt tggtgatgac tggcaggcga tttaccgatt
    31561 cagcggtgcg caaatgtcgc ttaccacggc tttccatgaa aactttggtg aaggtgatcg
    31621 ctgtgattta gacacgacct accgttttaa cagtcgtatc ggtgaggtgg caaacaggtt
    31681 tattcagcag aacccaggcc agcttaaaaa gccgctaaac agcttaacca atggagacaa
    31741 aaaagccgtc acgttattgg atgagggtca acttgacgct ttgctggata aactctctgg
    31801 ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc
    31861 cagcctggaa aaagcagcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca
    31921 tgccagcaaa gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtga
    31981 tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga
    32041 ggatttcccg gacgctgaag aacggcggtt aatgtacgtg gcgctgaccc gggcacgcca
    32101 tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa
    32161 tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa
    32221 tattacttca gacggtccgc gagataacgc tgataatcgg ggatcagaat atcgaccgcg
    32281 tcgttgaaat gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcaaccgga
    32341 atgttccata ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggaacggca
    32401 tttagtgggt cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca
    32461 acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat gccggttgca
    32521 cgggaaatca agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt
    32581 tgatgtcgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca
    32641 atatgtttcc gcgcaggtaa agtgcgtgtc gtcagttcca taatgtacat ccgtagttaa
    32701 ctttcctaca gattactgta agcacttatc gctgcaagag aaagaccgaa aaagcctgcg
    32761 cacaggcaca aaaatctcag gaagatggtt gtttttccgc ccattgcaga aaagtatttc
    32821 gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag
    32881 actgcggtgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca
    32941 tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct
    33001 cgacctcata atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg
    33061 gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg tgcggcatca aaatggttag
    33121 cttcccgctc attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat
    33181 taaaactgac caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa
    33241 tcgtcttctc aacgcgaaac actaactgat gcggtccgtt atccagttca atgctgtccg
    33301 caccgcgcag cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg
    33361 ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg
    33421 tcacgatgcc ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc
    33481 aaaatttttc tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat
    33541 tcattcgtat tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc
    33601 tggaggaaat aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg
    33661 cgctggtggg ggcaagcgac aaacccgatc gtcccagcta tcgcgtgatg aaatatctac
    33721 tcgaccaggg gtatcacgtc attccggttt cgccaaaagt tgctggcaaa acactgctgg
    33781 gccagcaggg gtatggcacg ctggcagaag ttccggaaaa agtcgatatg gtcgatgttt
    33841 tccggaattc agaagcggcg tggggcgtag cgcaggaagc gatcgccatc ggcgcgaaaa
    33901 ccctctggat gcaactgggg gtgattaatg agcaagcggc agtactggct cgggatgccg
    33961 gattaaatgt agtgatggat cgctgcccgg ctattgagat ccctcgcctg ggcctggcca
    34021 aataaaaaat ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca
    34081 gacgcggggc ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt
    34141 caggatgttc atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg
    34201 gtaggccgtt atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat
    34261 tcaccgccag ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga
    34321 ccactccgag ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg
    34381 caatcatagt cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt
    34441 caagttacat gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga
    34501 tcaccggatg atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat
    34561 caattgcggc atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg
    34621 agaaagtcag gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt
    34681 tataaccacg acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca
    34741 tcacgataac atcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat
    34801 catcacggac aaactctgcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg
    34861 caatatccag cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca
    34921 atgccgatac ggcgaaacca ccggtatagg agaaacagtt aagtacgcgt ttattttcga
    34981 cgtagcggcg ggtagccagg cggctgtcac gctggtccag gtagtagccc gttttgtgtc
    35041 cgtgctgaat atccaccagc agtttcatgc cgtgttcttc aatcggcagc agggcaggcg
    35101 gcaactcgcc ggtgatgggg ccctgagtca gctccatccc ttctttttta cgtaccgcga
    35161 cgtcgctgcg atcgtaaata gcacattccg ggtacagcgt ttgcagggcg ctaattaatg
    35221 ccgcgcgctg atactctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac
    35281 gatcgatagt aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga
    35341 ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc ttgctgcaaa cggcgggtaa
    35401 aaaaagcaat gtcgatagac tctgacgggt caaacgtcca gacgcgcgcc cggatttgcg
    35461 aagctggcga ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg
    35521 tttcaccgag gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat
    35581 ggcgacgaag taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt
    35641 acttttctgt cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa
    35701 cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctgggttt
    35761 acgggcgagt tcagggcgtt ggatttcgct ataccacgca gtacgaagcg aaaaaactgg
    35821 gattagccgg gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg
    35881 aagaagggca ggtggaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc
    35941 gtgtggaacg ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta
    36001 tccgttaaat acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg
    36061 gacctttcgg aaacagtcgg tataaatagc ggctgtttcc tttttcttcg ccaaatttat
    36121 tcgccatcgc ttttaccagc atacgaatcg ccggagaagt attgaattcc agatagaaat
    36181 cacgcacaaa acgcaccact tcccagtgtt ctggcgacag cgtaatccct tcgttctctg
    36241 caatcaccac tgccagcggc tcactccact ggctgctttc tttgagatag ccttcggtat
    36301 ccgtttctat ctctttacct tcgaagatca gcataattat tactacctta atcagactgg
    36361 cggcagtgta acaaaaaaca aagccccgca taaagcgagg ctcggtaagt aaccttgggc
    36421 gagattaatc gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca
    36481 gcgaaacata caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt
    36541 tgctggtttc aaacaaaata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca
    36601 gatgcagagc aggcagttgc aggaagatat tagcaaccat gccaatcagc accaccacaa
    36661 tacccgccat cagcataccg ccaaggaacg acatatcttt gcgggtggtc agcacatacg
    36721 cagagcagca gaagaacacc aacgccgttc cgcccagtgc catagcgatt acgtcaccca
    36781 ttccggcaga caggtaggtg ttcagaattg gtccgaggat atatcccaga aaaccggtaa
    36841 aggcgaatgc ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca
    36901 aaccatacat acccaccagc gtcagaatca gacctggaga tggcagcatc agcaccgtac
    36961 tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct cagcataaaa taggtattac
    37021 gcagcacctt atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat
    37081 ccattgagtc actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa
    37141 gggcacccag aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga
    37201 ttatcaggtc attaagcaaa tataacgccc tgagaatttc gacaggcaaa agaaaaaggg
    37261 gttagcagtt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    37321 accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg
    37381 aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc
    37441 tgaaaaacag tcaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca
    37501 ccatgaattc cttctctttt actcgtttag caaccggcta aacatcccca ctgcccggca
    37561 aaaagaaaaa taggtccatt tttatcgcta aaagataaat ccacacagtt tgtattgttt
    37621 tgtgcaaaag tttcactacg ctttattaac aatactttct ggcgacgtgc gccagtgcag
    37681 aaggatgagc tttcgttttc agcatctcac gtgaagcgat ggtttgcctt gctacaggga
    37741 cgtcgcttgc cgaccataag cgcccggtgt cctgccggtg tcgcaaggag aagagacgtg
    37801 cgatatgaat aacgaggaaa cattttacca ggccatgcgg cgtcagggcg ttacccggcg
    37861 cagctttctc aaatattgta gtctggctgc cacgtcgctg ggattaggcg cgggaatggc
    37921 accaaagatt gcctgggcgc tggagaacaa accgcgcatt ccggtggtat ggatccacgg
    37981 tctggaatgc acctgctgta ccgaatcttt tatccgctcc gctcacccgc tggcaaagga
    38041 cgtcatcctt tccctgattt ccctcgatta cgacgatact ttgatggctg ccgccggaac
    38101 ccaggcggaa gaagtctttg aagacatcat cacgcaatac aatggcaaat atatcctcgc
    38161 cgttgaaggt aatccgccgc tgggcgagct ggggatgttc tgtatcagca gcggtcgacc
    38221 gtttattgag aaactcaaac gtgccgctgc cggagccagt gcgattatcg cctggggaac
    38281 ctgcgcgtcc tggggctgcg tgcaggccgc gcgacccaat ccgacgcagg caacgcctat
    38341 cgacaaagtc atcaccgaca aacctattat caaagtacct ggctgcccac cgatcccgga
    38401 tgtgatgagc gccatcatta cttacatggt gacctttgat cgcttgccag atgtcgacag
    38461 aatgggccgt ccgttgatgt tctatggtca gcgaatccac gataaatgct atcgccgcgc
    38521 tcacttcgac gccggagagt tcgtgcagag ttgggatgat gacgctgccc gcaaaggtta
    38581 ctgcctgtac aaaatgggct gcaaagggcc taccacctat aacgcctgtt cctccacacg
    38641 ctggaacgat ggcgtttcct tcccgatcca gtcaggtcac ggctgcctgg gttgtgcgga
    38701 aaatggtttc tgggatcgcg gttcgttcta cagccgcgtg gtcgatattc cacaaatggg
    38761 tacccattcc accgccgaca ccgtcggttt aaccgcgctt ggcgtggtgg cagcggctgt
    38821 tggtgtgcac gcagtcgcca gcgccgttga ccagcgcaga cgtcataacc agcaacctac
    38881 agaaaccgaa catcagccag gcaatgagga taaacaggca tgagcactca gtacgaaact
    38941 cagggataca ccatcaacaa tgccggacgc cgcctggtgg tcgacccgat tacgcgcatt
    39001 gaaggccaca tgcgctgcga agtgaatatt aacgatcaga atgtgatcac caatgccgtc
    39061 tcctgcggca ccatgtttcg cgggctggag atcatcctgc aagggcgtga cccgcgcgat
    39121 gcgtgggcgt tcgttgaacg tatctgcggc gtctgtactg gcgtacacgc cctggcttcg
    39181 gtttacgcca tcgaagatgc catcggtatt aaagtgccgg ataacgccaa tatcatccgc
    39241 aacattatgc tggcaacgct ctggtgccac gatcatctgg tgcacttcta tcagcttgcc
    39301 gggatggact ggatcgatgt gttagatgcg ctgaaagccg acccgcggaa aacctccgaa
    39361 ctggcgcaaa gcctctcctc atggccgaaa tcatcccctg gctatttctt cgacgtacaa
    39421 aaccgcctga agaaattcgt tgaaggcggg cagttgggga tcttccgcaa tggctactgg
    39481 gggcacccgc agtacaaact gccgccagaa gccaacctga tgggctttgc ccactatctc
    39541 gaagctctcg atttccagcg tgaaattgtc aaaatccacg cggtctttgg tggtaaaaac
    39601 ccgcatccaa actggattgt cggcgggatg ccttgcgcca ttaacattga cgaaagcggc
    39661 gcagtcgggg cggtcaatat ggaacgcctg aacctggtgc agtcgatcat tacccgtacg
    39721 gcggatttca ttaacaacgt gatgatcccc gacgccttag ccatcggcca gttcaacaaa
    39781 ccgtggagcg aaatcggcac gggtctttct gataaatgtg tcctcagcta cggcgcattc
    39841 ccggatattg ccaacgactt tagtgagaaa agtctgctga tgcctggcgg cgcggtgatt
    39901 aacggcgact tcaacaatgt gctgccagtg gatttggttg atccgcagca ggtgcaggag
    39961 tttgtcgacc acgcctggta tcgttatccc aacgatcagg tggggcgtca tccgttcgat
    40021 ggcattaccg acccgtggta caaccccggc gatgtcaaag gcagcgatac caacattcag
    40081 cagctgaatg aacaggaacg ctactcgtgg atcaaagcgc cacgctggcg cggtaacgcg
    40141 atggaagtgg ggccgctggc acgcacgtta atcgcttatc acaaaggcga tgctgcgacc
    40201 gttgagtcgg tcgatcgtat gatgtcggcg ttgaaactgc cgctttccgg catccagtca
    40261 acgttgggcc gcattttgtg tcgcgcgcac gaagcgcagt gggccgcagg taagttgcag
    40321 tatttcttcg ataaactgat gaccaacctg aaaaacggca atcttgccac cgcttccacg
    40381 gaaaaatggg aaccagcaac ctggccgaca gagtgccgtg gtgtcggttt taccgaagcg
    40441 ccgcgcgggg cgttaggcca ctgggctgcc attcgcgatg gcaagattga tctctaccag
    40501 tgcgtggtgc cgaccacctg gaacgccagc ccgcgcgatc ccaaagggca gattggcgct
    40561 tatgaagcgg cgctgatgaa caccaaaatg gcgatccccg agcaaccgct ggagatcctg
    40621 cgtactctgc acagctttga cccgtgcctc gcctgttcaa cacacgtgct gggcgacgac
    40681 ggtagcgagc ttatctccgt gcaggtgcgt taacagcgaa ggagaatcat catgcaacag
    40741 aaaagcgaca acgttgtcag tcactatgtc tttgaagcac ccgtgcggat atggcactgg
    40801 ctgacagtgt tatgcatggc ggtgttgatg gtcaccggtt actttatcgg caagccgcta
    40861 ccttccgtca gcggcgaggc gacgtatctg ttctatatgg gctacatcag gttaattcac
    40921 ttcagcgccg ggatgatttt taccgtggtc ttgctgatgc ggatctactg ggcttttgtt
    40981 ggcaatcgtt actcccgcga gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag
    41041 ggcgtgtggt atggaatccg ctggtatctg tttctggcaa aacgtccgag tgccgatata
    41101 ggccataacc ccatcgccca ggcggcgatg ttcggctatt tcctgatgtc agtttttatg
    41161 atcatcaccg gctttgcgct gtacagcgaa cacagccagt acgctatttt tgcgccgttc
    41221 cgttatgtgg tggaattttt ctactggacg ggtggcaact caatggacat tcacagctgg
    41281 catcggctgg ggatgtggct gattggcgcg tttgtgatcg gtcatgtcta catggcgctg
    41341 cgtgaagaca tcatgtccga cgacacggtg atctccacta tggtcaacgg ctaccgtagc
    41401 cacaaatttg gcaaaataag taacaaggag cgttcatgag cgagcaacgc gtggtggtaa
    41461 tggggctggg taacctgctg tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc
    41521 tgtatgccca ttaccactgg cccgagtatg tggagattgt cgatggcggc acccaaggac
    41581 tgaacttgct ggggtatgtc gaaagcgcca gtcatctgtt gattctcgat gccattgatt
    41641 acgggctgga acccggaacg ctacgaacgt atgccggaga acgcattcca gcttatctca
    41701 gcgcgaagaa aatgagcctg caccagaaca gtttctccga agtgctggcg ctggcagata
    41761 tccgcggaca tctgccagca tatattgccc tcgtcggtct gcaacccgcg atgctcgacg
    41821 actacggcgg cagcctgagc gaactggcgc gggagcaact gcccgctgcg gaacaggcgg
    41881 cgctggtaca gctggcggcc tggggaattg taccgcaacc gactgaggag ttgcgctgtc
    41941 tcaattatga ctgtctgtcg atggaaaatt acgaaggcgt tcgcttgcgt cagtacagga
    42001 tgaggctgga gaaacaggga tgagcaacga cacaccattt aatgcgttgt ggcaacgaat
    42061 gctggcacgt ggctggacgc cggtcagtga atgccgtctt gacgactggc ttacgcaagc
    42121 gccagacggc gtggtgttat taagtagtga cccgaaacgc acgccagaag tcagcgataa
    42181 cccggtaatg attggcgaat tactgcgcga gtttcccgac tatacatggc aggtagcgat
    42241 tgctgatctt gagcagagcg aagccatcgg cgatcgcttt ggcgtctttc gctttcccgc
    42301 cacattagtg tttaccggcg gaaactatcg cggcgtgctg aatggtattc acccgtgggc
    42361 ggaactgata aacctgatgc gcgggcttgt cgaaccgcaa caggagcgtg cttcatgagc
    42421 gaaacttttt tccatctgct ggggccagga acgcaaccga acgatgacag tttcagcatg
    42481 aatccactgc cgatcacctg tcgggtgaat ggtgaaccga gtatggcggc cctggagcaa
    42541 tgtgctcaca gcccgcaggt gattgcgctg ttaaacgagt tacaacatca actaagcgaa
    42601 cgccaaccgc cgttgggcga ggtgctggca gtcgatctgt taaatctcaa cgccgacgat
    42661 cgtcacttta tcaatacgct tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct
    42721 gacgacagtg aaagtgaaat tcaggaggcg atcttctgcg gattatggcg ggtgcgcaga
    42781 cgtcacggcg aacagttgct ggaagacaaa ctggaggctg gctgcgcacc gctggcgttg
    42841 tggcaggcgg caacgcaaaa cgtcttgcca acagactcgt tgctaccgcc gcccattgat
    42901 ggcctgatga atggcctacc gttggcccat gaattactgg cacatgtacg taaccccgac
    42961 gcgcagccgc acagcattaa tctgacgcaa ttacccatca gcgaagctga ccggcttttt
    43021 ctctcacgtc tctgtgggcc gggaaatatt cagattcgta ccattggcta tggcgagagc
    43081 tatatcaacg ccacggggtt acgccatgtc tggcatttac gctgtacgga caccttaaaa
    43141 ggcccgttac tggaaagtta tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca
    43201 gaagatttgg tcgactctgc gcagcggctt agcgaggtat gccagtggct ggcggaaggt
    43261 gcaccgacgt aaaaagacgg taagtatcgc tttcaggctt atgaatatcg caatcggcga
    43321 atacctctgg tcgtagagtt tcaggataaa gagggagatc taccattaac gggttatttt
    43381 tctctcttcg cctacaggag tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt
    43441 tgctctgacc gcgctgtatc actttttatt tgtacccctt accctggggc tgattttttt
    43501 gctggctatc atggaaacca tttacgtggt caccggcaaa acaatctacc gcgatatgac
    43561 gcgcttctgg ggtaagctct tcggtatcaa ctttgctctt ggcgtggcta ccggcctgac
    43621 catggagttt cagtttggta ctaactggtc attctattcc aactatgtgg gcgatatttt
    43681 tggcgcaccg ctggcgatgg aagcattaat ggccttcttc ctcgaatcca cctttgtcgg
    43741 gctgttcttc ttcggctggc agcggctgaa taaataccag cacctgctgg tgacgtggct
    43801 ggtggcgttc ggttcaaatc tctctgcgtt gtggatattg aatgccaacg gttggatgca
    43861 atacccgacc ggtgcgcatt ttgatatcga caccctgcgt atggagatga ccagtttcag
    43921 cgagctggtc tttaacccgg tcagccaggt gaaatttgtg cacaccgtaa tggcgggata
    43981 cgtgaccggg gctatgttta ttatggcgat cagcgcctgg tatttactgc gcggacggga
    44041 gcgcgatgtc gcattacgct cgtttgccat cggttccgtc ttcggtactc tggcgattat
    44101 cggtacgctg caactcggag acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa
    44161 actggcggcg atggaagggg agtggcaaac ggaacctgca cctgcaccgt tccatgtggt
    44221 tgcctggccg gaacaggatc aagagcgtaa cgcctttgct atcaaaattc ccgcgctgct
    44281 aggaatcctc gccactcatt cattagataa acccgtgccg ggtctgaaga atttgatggc
    44341 tgaaacctac ccacgcttgc aacgcggacg tatggcgtgg ctgttaatgc aggaaatatc
    44401 gcaaggcaat cgtgagccgc atgtgttgca ggcattccgg gagctggaag gcgacctggg
    44461 ttacggcatg ttgctctccc gctatgcgcc ggatatgaat catgtcacag ccgcacagta
    44521 ccaggcggcg atgcgtggcg cgatacctca ggttgcgccg gtattctgga gtttccgcat
    44581 catggtgggc tgtggttccc tgctgctact ggtgatgctg attgcgcttg tccagacgct
    44641 gcgtggcaaa atcgaccagc atcgctgggt gctgaaaatg gcgctctgga gtttgccgct
    44701 gccgtggatt gcgattgaag ccggatggtt tatgaccgag tttggtcgtc agccgtgggc
    44761 gatacaagac atcttaccga catactccgc gcactccgcc ttaaccacag gacaactggc
    44821 tttctcactg atcatgatcg tagggcttta caccctgttc ttaatcgccg aagtctacct
    44881 gatgcagaaa tatgcccgtc tggggccgag cgcgatgcag agtgaacaac cgacgcagca
    44941 acaggggtaa aggagaaaat catgtttgat tatgaaacat tgcgcttcat ctggtggctg
    45001 ctgattggcg tgatcctggt ggtctttatg atctccgacg gatttgacat ggggatcggc
    45061 tgtctgctgc cgctggtggc gcgtaatgat gatgaacgtc ggatagtgat aaacagcgtt
    45121 ggcgcgcact gggaaggcaa tcaggtctgg ttgatccttg caggtggggc attatttgcc
    45181 gcctggccca gagtgtatgc agcggcgttt tccggctttt atgtggcgat gattctggtg
    45241 ctgtgctcgc tgttcttccg cccgctggcc tttgattatc gcggaaaaat cgccgatgca
    45301 cgctggcgta aaatgtggga tgccggactg gtcatcggca gtctggtgcc gccggtagtc
    45361 ttcggtattg cattcggcaa cttgctgctc ggcgtgccgt ttgctttcac accgcaatta
    45421 cgcgtggaat atctcggcag cttctggcaa ctgctgacgc cattcccttt attgtgtgga
    45481 ttgctcagtc ttgggatggt gattttgcaa ggtggcgtct ggttacaact gaaaactgtt
    45541 ggtgtgattc atctgcgttc acagctggcg accaaacgcg ccgcactact ggtgatgctg
    45601 tgctttttgc tggcgggtta ctggctgtgg gttggtattg atggctttgt actgctcgcc
    45661 caggatgcta acggtccttc caatccgcta atgaaactgg tggcagtgct acctggtgcc
    45721 tggatgaata atttcgtcga atcgcccgtt ttgtggatct tcccgctgct ggggttcttc
    45781 tgcccattgc tgacggtgat ggcgatttat cgtggtcgcc cgggttgggg atttttgatg
    45841 gcgtcattga tgcaatttgg cgtgattttc acggcaggca tcacgctgtt cccctttgtc
    45901 atgccgtcaa gcgtgagtcc gatctccagc ctgacgttgt gggacagcac ttccagtcag
    45961 ctgacgctga gcattatgtt ggtaatcgtg ctgatatttt tgcccattgt gttgctctac
    46021 actctctgga gctactacaa aatgtggggg cgcatgacga cagaaactct ccgccgtaac
    46081 gaaaacgagc tgtactaagg agcaggaaca atgtggtatt tactttggtt cgtcggcatt
    46141 ttgttgatgt gttcgctctc cacccttgtg ttggtatggc tggacccgcg tctgaaaagt
    46201 taacgaacgt aagcctgatg tggcgcatta gcatctcatc aggcaatcaa taatgtcaga
    46261 tatgaaaagc ggaaacatat cgatgaaagc gatcttaatc ccatttttat ctcttctgat
    46321 tccgttaacc ccgcaatctg cattcgctca gagtgagccg gagctgaagc tggaaagtgt
    46381 ggtgattgtc agtcgtcatg gtgtgcgtgc accaaccaag gccacgcaac tgatgcagga
    46441 tgtcacccca gacgcatggc caacctggcc ggtaaaactg ggttggctga caccgcgcgg
    46501 tggtgagctg atcgcctatc tcggacatta ccaacgccag cgtctggtgg ctgacggatt
    46561 gctggcgaaa aagggctgcc cgcagcctgg tcaggtcgcg attattgctg atgtcgacga
    46621 gcgtacccgt aaaacaggcg aagccttcgc cgccgggctg gcacctgact gtgcaataac
    46681 cgtacatacc caggcagata cctccagtcc cgatccgtta tttaatcccc taaaaactgg
    46741 cgtttgccaa ctggataatg cgaacgtgac tgacgcgatc ctcagcaggg caggagggtc
    46801 aattgctgac tttaccgggc atcggcaaac ggcgtttcgc gaactggaac gagtgcttaa
    46861 ttttccgcaa tcaaacttgt gccttaaccg tgagaaacag gacgaaagct gttcattaac
    46921 gcaggcatta ccatcggaac tcaaggtgag cgccgacaat gtctcattaa ccggtgcggt
    46981 aagcctcgca tcaatgctga cggagatatt tctcctgcaa caggcacaag gaatgccgga
    47041 gccggggtgg ggaaggatca ctgattcaca ccagtggaac accttgctaa gtttgcataa
    47101 cgcgcaattt tatttactac aacgcacgcc agaggttgcc cgcagtcgcg ccaccccgtt
    47161 attggatttg atcatggcag cgttgacgcc ccatccaccg caaaaacagg cgtatggtgt
    47221 gacattaccc acttcagtgc tgtttattgc cggacacgat actaatctgg caaatctcgg
    47281 cggcgcactg gagctcaact ggacgcttcc aggtcagccg gataacacac cgccaggtgg
    47341 tgaactggtg tttgaacgct ggcgtcggct aagcgataac agccagtgga ttcaggtttc
    47401 gctggtcttc cagactttac agcagatgcg tgataaaacg ccgctgtcat taaatacgcc
    47461 gcccggagag gtgaaactga ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg
    47521 ttcgttggca ggttttacgc aaatcgcgaa tgaagcacgc ataccagcgt gcgctctcca
    47581 tcaagacaaa taaacatttg ccgctgttgg tccacgcagg ccatttacgc ggcaaaattc
    47641 cacgcgtaat cctggtataa gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac
    47701 ctggacttct ttacgaccgt cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa
    47761 ggttttgaca attcctgtca ttttacggga caaacaaatt ccttactgaa aatactgcgc
    47821 tgcactatac ggggttaata aaataaagcc agcgatattt aagaccgccg gacggctaaa
    47881 ataaaatttg cttaatctca attatcatgc gttaatagct gcgtcggttt gaaagacaga
    47941 cagcatacaa agtagtttac taaagcagtt ctcattatca ggcattatcc ccttcttttg
    48001 agtctctctc ctgaacacta agtagtttct gtattaaagc cctgtttgcc gaaaggccca
    48061 aaatgaagga agtaaaatat gtctaataaa atgactggtt tagtaaaatg gtttaacgca
    48121 gataaaggtt ttggctttat cactcctgat gatggcagca aagacgtttt cgtccatttc
    48181 actgccatcc agagcaatga attccgcacg ctgaacgaaa atcagaaagt tgaattttct
    48241 attgagcagg ggcaacgtgg tcccgcggca gcgaacgttg ttacgctcta aggttgccgt
    48301 tattactcaa catctccatt tccgctgtcc atgttgccat ggttcacagt accgcacatc
    48361 ggcattcgat gtgacggagc gaaacccttt gggtgcaaag tgcatttttt gtaaatcgac
    48421 gatgatcacc tttgataacg tcgcgctgca aatacgcact gaccatgcgc cgctggattt
    48481 cacaaaataa tatcaggctc cctcgtggag ccttttttat atctgcctta tttttcttca
    48541 atgctgtacg tatagtaagc gataacctgt tgattattga atctttcggg gagatggctt
    48601 ataacatttc ttacctgacc agggtaccgg gaaccaacac cttactggcg tgttgctgtc
    48661 ttttaagacc agaagaggtt aacagtgaat attgaagagt taaaaaaaca agccgaaacg
    48721 gaaatcgccg actttatcgc gcaaaaaatc gccgagctga acaagaatac agggaaagaa
    48781 gtctctgaaa ttcgcttcac cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc
    48841 aaaatcaaaa taatgtgatt ttgtgaacat caccccgtgc gaggtgatgt tccgcttgtt
    48901 gctaatttag tgaccaatca ttggcgattg cggaattaag cgttggtaca attcctccgg
    48961 caccgggctt tgccatactc ccgcatagat tgcgtaacca atcgccgcaa acataatccc
    49021 cataaccagt agcgtcatta accatccaga caacgcaaag gcttttttat ttgccgcagg
    49081 tttttgcagt gaaaaggtca atgttgaggc taccggacat gactctacgc aagtcataca
    49141 gccggtacat tccactgttc gtacctgaat taatttatcg accgggatcc gtgatgggca
    49201 actttttgcg catttgccac aatcgataca actttcggca ttgcgacgaa tcttaaacgg
    49261 cgataatagc gaaaccacgc ccatcagcgc gccatatggg caaagataac gacaccaggc
    49321 atggcgaata aacaggctgg caatcagcaa aacgatcatg ctgattaatg tcgcggtccc
    49381 catatgacga aagaaatcga gcattttaac gtccatcacc acgctgtagg gcgacaacat
    49441 aaaatagtga atcgcctgag cgggcatcaa taacgcgata tagagaaaaa aactcaacag
    49501 caaatacttc acgccgcgca gaggaatatc cagccagcgg ggaaggacaa attgccgacc
    49561 aaacagtttg ttaccgagat cgccgattaa ttcagaaagc gtaccaaccg ggcataacca
    49621 tgaacaaaag gcctttttga gtaatagact gatgacgata aaagcgacca ataacagcat
    49681 cgcggcggcg tggacggacg gtaactgacc tgttgcaagg ctatatttca gattcatcag
    49741 accggcaatc ggtagccagc cttcgatacc tcccggtctg gcgacaaatg tcgtactacc
    49801 tgccgtttcg taatagcgca cccaatacca gaacgtgatg gcaatataaa tattaattgc
    49861 caacagtaat aattgcgtcg ctttacgcca ggtcgtggca ttgcgccagt cattccacgg
    49921 taatttgccg cccgtcgtgc ctggccgccg ctgccagcgg gttctttttt tctctgccat
    49981 gattttgcca gtccgttaag ttgcatacaa aatgccacta ttctagttgt tcttaactgg
    50041 ctgatattga ttcaaatcgc gttcaggtct ttcttattaa ccatgcttcc agagcggcaa
    50101 cactgcgtgt aatttcttcg tgtggaaggg gggcagataa tggctgctgc tccagttgtg
    50161 cgcattgctg gctggcgcta cgcattccca gactcgagca actgcttttt agctgatgtg
    50221 cggcacgctt tattttctcg ttatcctgac tggcgcgggc aatgtcgatt tcatcgagaa
    50281 gcggcagggc atgttgttta aataatatca gccattcgtg gatcttctcc gcccccatta
    50341 aatgagcatc ttcattgagt tgcgatacat ccagcggttg atcgttattg acttgcagtt
    50401 ggagatagtg cgccagtaat tgaccgagca cttcacgcgg caccggttta gggataatcc
    50461 cgcgaaatag cgaactggta cgctgtcgca gtgtttcgtc aatgacatgg gcgctaaagc
    50521 caatcaaaac cagcgacgga tattgctgtg ccagttgtcg ggcaagcgta atgccgtcga
    50581 tatccggcag atcaaaatcc accagtgcgg cagcaaacgg ttcactattt tgcagtgttt
    50641 ctaaagcctg cacggcattg ccaacagcaa caacctgcgc accactgatg ttcagcatct
    50701 caacggtaat tcgctgggtt agcgggttat cttcaattaa cagcaaacgt aaaccgtcca
    50761 gacgcaccgc ctgattgact gtttttggca cgggtgcagt ggcaacacgt aacggcaagc
    50821 gtaaacaaaa acagcttcca acctccggcg tgctggtggc gctcagttcg ccgcccatcg
    50881 cctgggccag acggctactg atagtcagcc ccagcccggt gccgccgcgt ttgccgctaa
    50941 cctgaacaaa tggctggaag atttctgcca gtttcgcggg atcaataccg cagccgctgt
    51001 cttccacttc gaccagccat tgttcgccat cagtgcgact acgcaggata atgtgccctt
    51061 cgtcggtgaa acgcagggcg ttgctcaaca ggttggtgat gacctgacga atacgccgcg
    51121 gatcgcccat taacgcagtc ggtacatcgt cggcaatttc tgttgccagg cgaatcgggc
    51181 gacctttcac ccttccgctc attaattgca gggtactttc cagcagcggg cgcggttcaa
    51241 agggttcatc gctgaccgaa acattcttgc cacctgcttc gatagccgaa taatcgagaa
    51301 tatcgttgag gatggtcagc aacgattcac cagagtcggt aatcgcccgc aaatcatcgc
    51361 gctgggcgtt aagtgcgggg ttatctgcca gcagttgcgc ggtgccgaga ataccgtaca
    51421 gcggtgtgcg gatctcatgg ctcatcgccg ccagaaacgc cgattttgcc tggctggctt
    51481 tttctgcttc cgcccgtgcc tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac
    51541 gcgctttgac ctgcgccgcc agctgttcgc ggtggcgatt cagtgcatga acactgctgc
    51601 gaaacgcatc catcagccgc ccgatggtat ccagttcccg tacgccagcg gtttccggga
    51661 aaggggagtc gatatcacca tccagcaagc gttgcagcgc ctgcgtttgt tctgcaagag
    51721 ggcgcgtgac tgagcgataa accacgcgcc agaggatcag aatcagtgcg caaagagaaa
    51781 ctaatcccag caataacagg ctgtattgcc cgcgtgcact ggctttttcc agatgtgcca
    51841 gcccgtgctg attacgcagc tcaatggtgt cgaccagctg actgacttcg ctactaaact
    51901 gcgcgaattg ggcgatgtta ttttgggcga gagtttgcag gtgattgctg atttcactgt
    51961 cctgctgata cagcgccagc aaatcgctat attggctaac ggtagttaac gttgttgcta
    52021 cctgagtacg aacacccgga tcttcaatgc gtatttgccg acgttgcaga attttcaccg
    52081 cattattgag ctgcttttcc agcgttggcg catttttctg gatctgttcc agccccagat
    52141 tcatcaccat ttgctgcacc cgtagggcgc taaggcgcag ttcattcatc tggttaacat
    52201 actcaagatc gatatcaatc agccgatcga gtgcactttc tgcggcctga cgctgatgtt
    52261 gttcgatcaa atcgtaaatc ccggcctggg tcgctccggc ggaggttgcc gcattattcg
    52321 cctgaccttg cgccaggcgt gcgatctcat cggcggcggc gactatctgc tgactgagtt
    52381 gctgttgttg ctggcgcagt tgcaaacgct gtcccaccag ttccccttgc tgacgtaacg
    52441 aacgggagat ctcctgctcc tgttgttcaa tagcggtggt gtcaaaacct tgttcccgta
    52501 acgcttgcag caacgcatta atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct
    52561 gccacatctt ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc
    52621 tggcttcgct caactggcgc gccatattca tggtaggaat caatgcctga gtgttgtctt
    52681 tttccaccag gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta
    52741 acagcgccat cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg
    52801 gtgcacttta ggtgaaaaag gttgagtcgc aaagcagaat gcatctagca taaagcctta
    52861 ttattgatga ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttat
    52921 cggcattttc ggctgataac ctgttgcgct ggcatgatgc gcagcattac acggtgcaag
    52981 cctctacgcc gcttaaagcc aaacgcgcat ggaaactgtg cgcgctttat cccagcctga
    53041 aagattcata ctggttatcg ttgaactatg gtatgcagga ggctgctcgc cgctacggtg
    53101 tggatttaaa agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa
    53161 tcgaccaatg taaacagtgg ggcgcagagg ccattttgct cggtagtagc acgacgtcat
    53221 ttcccgatct gcaaaagcag gtagcaaatc tgccggtgat cgaactggtg aatgctattg
    53281 atgctcccca ggtgaaaagc cgcgttggtg tgccttggtt tcagatgggc tatcaaccag
    53341 ggcgatatct ggtgcaatgg gcgcacggta aaccactgaa cgtgctgttg atgcccggcc
    53401 ccgataacgc cgggggcagt aaggagatgg tagagggttt tcgcgcagcc attgccggaa
    53461 gcccggtacg tattgttgat attgcgctcg gtgataacga tattgaaatc cagcgtaacc
    53521 tgttgcagga gatgctggaa cgccatccag aaatcgacgt cgttgccgga acggccattg
    53581 cggcagaggc ggcaatgggg gaagggcgca acctgaaaac gccgcttacc gtggtgtcgt
    53641 tttatctttc acatcaggtg tatcgcgggt tgaagcgggg aagagtgatt atggcagcca
    53701 gcgatcaaat ggtctggcag ggggaactgg cggttgagca ggctatcagg caattacagg
    53761 ggcaatcggt gtctgataat gtcagcccac cgattttagt catgacgccg aaaaatgccg
    53821 accgcgaaca tatccgtcgc tcgctgtcac cagggggatt tcgtccggtc tatttttatc
    53881 agcacacatc agcggctaag aaataacctt cgccatgttg tgtcaccagt aaatctgcac
    53941 tgagtttatg gcgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca
    54001 cccgacgcgc agagagcata cgcagcagac gttcacggct gagaatttcg ccgggattgg
    54061 tcacaaacgc caccagcatt tcatactctg cgcgggtcag tttaatcggc tctccatccc
    54121 gctccagcgt atggcgcgac acattcaggc aataaccggc aaagcgatag cagttgtcct
    54181 gagtgtgcgg ttgcgcctgt cgcgcgaggt cgattcgcca gagcaaattt ttcactcgta
    54241 ccaccagttc gcgcagctcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc
    54301 caacaatacg gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc
    54361 gttctcgcag ggcgcgggtt aacatcaggc cattttcatc gggcaaattg atatccagca
    54421 gaatcaaatc caccggctga ttctgcataa tttcccgcag cccggcaccg ctcgccgtaa
    54481 cggaaacggt atacccctcc tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct
    54541 catcttcaac aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatc
    54601 tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc cgctgttcat atttgctcat
    54661 taagatcgct tcactaaacc ataattttac aggggctatt atgcggaaac tctggaacgc
    54721 gctacgccga cccagtgctc gttggtcggt actggcgctg gtcgctactg ggattgtgat
    54781 tggcattgcg ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt
    54841 ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt
    54901 ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc
    54961 aggcatggtg aagcgcaaac tggaagcgag caacgacatt taccagacct ttattgccca
    55021 ctccattgat acacctgaga aatttgaagc caaacgcgcg gaacttgccg agcgtgaatg
    55081 ggcgcgaatg aaagaaaaca actcggcaac ctgccgctcc tgccataact acgatgcgat
    55141 ggatcatgcg aagcagcatc ctgaagcggc gcgtcagatg aaggtggcag cgaaagataa
    55201 tcaatcctgc atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagtgg
    55261 cttccgtaag cagttcgatg agctgcgcgc cagtgctaat gatagtggtg acacgctgta
    55321 ctccattgat attaagccga tttatgcggc gaaaggcgat aaagaagcgt ctggttctct
    55381 gctgcctgct tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat
    55441 cactggctgg acggaaagtg caggacgtca gcgtgtactc acccaattcc caggtaaacg
    55501 catctttgtt gcctcgattc gtggtgatgt gcagcagcag gtgaaaacac tggagaaaac
    55561 caccgttgcc gacaccaata ccgagtggag caagttgcag gccactgcgt ggatgaagaa
    55621 aggcgacatg gtgaacgata tcaaaccgat ctgggcttat gcggattcgc tgtacaacgg
    55681 cacctgtaac cagtgccacg gcgcaccgga aatatcccac tttgacgcta acggttggat
    55741 cggcacgctc aacggcatga ttggctttac cagcctcgat aaacgtgaag aacgcacctt
    55801 gttgaaatat ctgcaaatga atgcgtctga caccgcaggt aaagctcacg gcgataagaa
    55861 ggaagaaaaa taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca
    55921 caactcggcg gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt
    55981 gcgactgcgg cgcaagcggc gactgaggct gtcatctcga aagagggcat tcttaccggg
    56041 tcgcactggg gggctatccg cgcgacggtg aaggatggtc gctttgtggc agcaaaaccg
    56101 ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat tgccggatca tgtacacaac
    56161 gcggcgcgta ttcgttaccc gatggtacgc gtggactggc tgcgtaagcg ccatctgagc
    56221 gacatctccc agcgcggtga taaccgtttt gtgcgcgtga gctgggatga agccctcgac
    56281 atgttctatg aagaactgga acgcgtacag aaaactcacg ggccgagtgc cttgctgacc
    56341 gccagtggtt ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct
    56401 attgccttgc atggtaatag cgttggtacg ggcggagatt actctaccgg tgctgcgcag
    56461 gtgatcttgc cgcgcgtggt tggctcaatg gaagtgtatg aacagcaaac ctcctggccg
    56521 ctggtattgc agaacagcaa aaccattgtg ctgtggggct ccgatttgct gaaaaaccag
    56581 caagcgaact ggtggtgccc ggatcacgat gtttatgaat attacgagca gctaaaagcg
    56641 aaagtcgccg ccggtgaaat tgaggtcatc agcatcgatc ctgttgtcac atccacccat
    56701 gagtatctgg ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgacgtgccg
    56761 ctgcaactgg cgctggcgta tacgctgtac ggtgaaaacc tgtacgacaa aaacttcctc
    56821 gctaactact gtgtaggttt tgagcagttc ctgccgtatc tgctgggtga gaaagacggt
    56881 cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt
    56941 gggctggcgc ggcagatggc ggcgaacaga acgcagatta ttgctggttg gtgcgtacag
    57001 cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg
    57061 caaattggcc tgccgggtgg tggcttcggt tttggctggc actataacgg cgcaggcacg
    57121 ccggggcgta aaggcgttat tctgagtggt ttctccggtt ctacgtcgat tccgcctgtt
    57181 cacgacaaca gtgactacaa aggctacagc agcaccattc cgattgcccg ttttatcgat
    57241 gcgatcctcg aaccggggaa agtgatcaac tggaacggta aatcggtaaa actgccgccg
    57301 ctgaaaatgt gtatttttgc cggaactaac ccgttccatc gccatcagca gatcaaccgc
    57361 attattgaag gctggcgcaa gctggaaacg gttatcgcca tagataacca gtggacctca
    57421 acctgccgct ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg taacgatctc
    57481 gaccagtacg gcaaccactc caatcgtggc atcatcgcca tgaaacaggt cgtgccgccg
    57541 cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc tgtgccgccg ctttaatcgc
    57601 gaagaagcct ttaccgaagg gctggacgag atgggctggc tgaaacgcat ctggcaggaa
    57661 ggtgtacaac aaggcaaagg acgcggcgtt catctgccag cgtttgatga cttctggaat
    57721 aacaaagagt acgtcgagtt tgaccatccg cagatgtttg ttcgccacca ggcattccgc
    57781 gaagatccgg atctcgaacc gctgggcacg ccgagtggcc tgattgagat ctactcaaaa
    57841 accatcgccg acatgaacta cgacgattgt caggggcatc cgatgtggtt tgagaaaatc
    57901 gaacgctccc acggtgggcc tggctcgcag aagtatccgt tgcatctgca atctgtgcat
    57961 ccggatttcc gacttcactc gcagttatgt gagtcggaaa ctctgcgtca gcaatatacc
    58021 gtagcgggta aagagccagt atttattagc ccgcaggatg ccagcgcgcg cggtattcgt
    58081 cacggtgatg tggtacgcgt ctttaacgtt cgtggtcagg tgttggctgg agcagtggtt
    58141 tctgaccgtt atgcacccgg cgtggcgcga attcacgaag gggcatggta cgatccggac
    58201 aaaggcggcg agccaggggc gctgtgcaaa tacggtaacc ccaacgtgtt gaccatcgac
    58261 atcggtactt cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag
    58321 aagtacaacg gaacagtgga gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg
    58381 cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga
    58441 ttgcctgtgt ttacgcctgg ctggcgcagt tgttctcccg tgaactggac gatgaacaac
    58501 tgacgcaaat cgccagtgcg cagatggctg aatggttttc gttgctgaaa agcgaacctc
    58561 cgctcactgc gacggtgaat gggctggaaa acagtattgc cactctgaca gtacgtgacg
    58621 atgcccgtct ggaactggcc gctgactttt gcggcctgtt tctgatgacc gacaatcaag
    58681 cggcgctgcc gtatgcatcg gcctacaaac aggacgagca agagattaaa cgcttgttag
    58741 ttgaggcggg gatggaaacc agcggcaatt tcaacgaacc ggcagatcat ctggcgatct
    58801 atctcgagtt gttgagtcat ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa
    58861 gaatcgacgg tttgcggcaa aaaacactga cggcgctgcg gcaatggtta ccggaatttg
    58921 ttgcgcgttg ccatcagtat gaccgctttg gtttttacgc agcactaagc cagttgttgc
    58981 aggtgttggt ggagtgcgac aaccaaaagg gataatgccg atttgtaggc ctgataagac
    59041 gcgttaagcg tcgcatcagg cattatggcg cagttgccgg atgcggcgag aacgccttat
    59101 ccggcctacg ggaacagtgc tttttgtggg cctgataaga tgcgttaagc gtcgtatcag
    59161 gcattagtgc gcagttgccg gatgcggcga gaacgcctta tccggcctac gggaacagtg
    59221 ctttttgtgg gcctgataag atgcgttaag cgtcgcatca ggcattatgg cgcagttgcc
    59281 ggatgcggca tgaacgcctt atccggccta cgggaacagt gctctttgta ggcctgataa
    59341 gacgcgttaa gcgtcgcatc aggcaacaaa ctcacgggtg agccacaaac cgggaaagcc
    59401 gctggcgcag caggcggttt tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg
    59461 ccaccgcgat ccccggccag tccagagcca gttcatgacg caggcgtacc gcgcgttgca
    59521 ccacaatggc ggcatggtcg tcaaataccc aggttgtctc ctggatctca ctcggttcaa
    59581 ccacccccaa accgacaatt tcattcaact cctcttcaga gatgccggta tgcaggcaaa
    59641 attcggtaat agtaaaagtc accgtaacat tagccattat gctttccccc aatctttacg
    59701 tggatcaaaa gacgactggg cgtctgccag ttgctgccac agcgcggcag tgttttcatc
    59761 cggtttcggc ggcatcacga ttttcagtac cgcatacaga tcgccggtct gttttttgct
    59821 caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc tggctgccag gcgggatagt
    59881 cagcaaaatg ctttctttca gtgttggaac ggtgacttta gtacccagcg ccgcttccca
    59941 cgggctaacc ggcaccacta tttccagatc ctggccgaca atatcaaaca gcggatgtgg
    60001 cgcaatgtga atcaccagcc acaggtcgcc atttggaccg ccgttttcgc ccggcgtccc
    60061 ctggcctttc aggcggatgc gttgaccatt gccgacgccc gccgggatct tcacattcag
    60121 cgttttcggg atttcctgtt cgatcatgcc aaaggcgtta taaaccggca ggttatagct
    60181 gatggtacgc ttatgctcag taagcgtttc ttcgaggaaa accgccactt cgatttcaat
    60241 atcgtggccg cgtgtggcgg ggcgttgacg gctctggcgg gcatgctgac cgaaaattga
    60301 cgagaagata tcgtcaaaat cttcggcgtt aaaactctga ccgtcgccat gatggaactg
    60361 acggttaaat tgcggatcgt tgcgatgttg ccacatctga tcatactcag cgcgacgttg
    60421 ttcatcactt aacacttccc aggcttcggc gacctctttg aagcgggctt cggcatccgg
    60481 ttctttgctg acatcaggat ggtatttgcg ggccagtcga cgataggcgg tcttgattgt
    60541 cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa taatccttta attccatagc
    60601 gttatctcgc gtaaatcaac acaaattgaa ggaacccctg taaggtaact cctataagtg
    60661 tagggtaatc ctcaaaattt catatgccaa cacagaatat gttattgaaa tcatcgcgga
    60721 ggggaggttg cctcaaggtg ggaggaacat attttattta cctcactcct gaacttttgg
    60781 ctcatgctta caagttaaac aataaggaaa ggaatgcaca actcgactat ttaaatcgtt
    60841 atgcaattta atgataaaga catgtgctaa aaaccattgc tattagtctc acattctttt
    60901 tttattggta atggtaacgc cgctgatttt ctttatagct actgatgtaa tcattagcgg
    60961 cgtaaacgag gccggggcta atcaggcata gtttgcgtca aaacttgcct gtttttaaag
    61021 gggaatatag aaaaacaggc gttcaacgag ccattttgcg aacctgttcc cggaaaaatg
    61081 acatatttct gtcacactct ttaattattg ataacaaaag aggtgtgagg aatgaacaaa
    61141 acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc tcaggcacaa
    61201 accgtaccgg aaggctatca gctacagcaa gtccttatga tgagccgtca taacttacgt
    61261 gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa atggcctgaa
    61321 tgggacgtcc ccggtgggca gctcaccact aaagggggcg tgctcgaagt gtatatgggc
    61381 cattatatgc gtgaatggct ggcagaacag gggatggtga aatcagggga atgcccgcca
    61441 ccggacaccg tttatgccta tgccaatagt ctgcaacgca ccgtcgcgac cgcgcagttc
    61501 tttattactg gcgcattccc tgggtgtgat attcctgttc atcaccagga aaaaatgggc
    61561 actatggacc ccacctttaa cccagtgatc accgatgatt ccgccgcatt cagtgaacag
    61621 gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag ctacaagcta
    61681 ctggaacaaa tcgttaacta taaagattct cctgcgtgta aagagaagca acaatgttcg
    61741 cttgtagatg gcaaaaatac ctttagcgcg aagtatcaac aagaaccggg cgtttccggg
    61801 ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta cgaaggtttt
    61861 ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa ggtgttgtcg
    61921 aagctgaaaa acggttacca ggacagcctg tttacctcac cggaagtggc gcgcaatgtt
    61981 gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcac cagtgcaccg
    62041 aaaatcacgg tattagttgg gcacgactcc aacattgcct ctctgttaac ggcgctggat
    62101 ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg caaaatcgtt
    62161 ttccagcgtt ggcatgacag caaagccaat cgcgacctga tgaaaattga atatgtgtac
    62221 cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc tgcgcagcgc
    62281 gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttttgccc aatggataag
    62341 tttgatagcg tattgaatga agcggtgaaa taacagaaaa ctcccccgcg tgaagcgggg
    62401 gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa agcgaatctt
    62461 tatcggtctt agcaaaggct ttgactaaca cttcatcgct accttcttcc caaatctttt
    62521 ccgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt tccggcgacg
    62581 tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga gttggcatgc
    62641 gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga catattcccc
    62701 ttgataacga gcaatagaca gttcttcctg gctgggctgg cgtgaaccgt caccgcctgc
    62761 gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa ataattcctg
    62821 cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg tccaggtgga
    62881 tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc tcgccagttt
    62941 tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg tacgcatttg
    63001 accggacatg ttaccaaagc gggtaggtgt accaaaaata atggcgtcgt aatcggccag
    63061 ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt caaataattg
    63121 cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca ctttgcttgc
    63181 accctcagca actgcgcgtg ccatcgtttc aatatgtccg tacatggaat aataaagcac
    63241 cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag cgattcgcta
    63301 ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc acttatttga
    63361 tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt ttatctcatc
    63421 gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata tctattctcg
    63481 ctaagaagct gttgcaggat attaccaaac gcgggtctgc ccgcgtcagt tcactaagct
    63541 tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct atgcaatatg
    63601 atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc cggcaatttt
    63661 gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggacagca cagcgggggt
    63721 aatttcaaaa atgacccgca gcgcgcatca gaagcaggta aaaaaggtgg caagagcagt
    63781 cacggtaaaa gcgacaacta gccgggctaa tcaatgacgc atgcattttt gtctgtagct
    63841 cgtcaaaacg ccatcaccgc cggttacccc ggcggttttc tgtttatggc tcctgatgaa
    63901 caacttctgg cggtggaacg tcaaccaacc ttctgcttaa caacgcattg agtaaaatcg
    63961 cgccaaaggt ggctgtaccg atccctccca acgtaaaacc gcccagtgtg agagcaaaat
    64021 cacccgcacc cagcactaag gttactgcga ccataatcaa attaccgttc tggcttaaat
    64081 ccacactgtt ttgtacccag atccgtgcgc ctgcgacggc aatcagcccg aacacaacaa
    64141 ttgatgcacc accaatcacc gcagccggaa tagtatggat cagcgcgcca aatttcggcg
    64201 aaaagcccaa cagcatggcg atgacggcag cggcaacaaa caccagcgtc gagtagactt
    64261 ttgtcacggc catcacaccg atattctcgg cgtaggtggt tacgccgcta ccgccgacag
    64321 agccagaaag catcgtcgcc agcccatcgc ctacgaatgc ccgccccata tacgggtcca
    64381 tattgcgtcc ggtcatcccg gcgaccgcct tgagatgacc taagttttcc gccaccagaa
    64441 tcaccgccac gggcgcaatc agcatcattg cctgaccatt aaaagcagga gtggaaaaat
    64501 gtggcagacc gaaccaggca gcatggctga cgagagtaaa atcgacggct tttcccagcc
    64561 ctaaaacgtt ggtcatcacg ccatacagca gacaggcgac aattaatcct acgagaatca
    64621 ataatcgctg gatcatgcca cgggtaaata cggccaccag cccaatacat agcaccgtca
    64681 ttaccgccat ccagctatca aaggccgaag ccgatacatt tttcactgcg ataggcgcta
    64741 agttcaggcc aatcgccatc actaccgcac ccgtcacaac cggcggcatt agtcgttcaa
    64801 tccagcgcgt accgattttc atcaccacca ggccaatgac ggtataaacc agcccacagg
    64861 cgataatccc gcccagcgcg atgctgatat tcggattaat tccctgacca ttaaagcccg
    64921 tcgcggcaat caccacgccg acaaaagccg cgctggaacc gagataactg ggcacgcgcc
    64981 cgccggtaat aaagaaaaac agtaacgtgc cgatccccga cattaaaatg gaaagattgg
    65041 gatccagccc catcagaatt ggcattaaca ccgtcgcgcc aaacatcgcc accgcgtgtt
    65101 gaacgcccat tactgccgtt tgagcaaacg gcaatcgttc atccggcgcg accacgccgc
    65161 tctctgtaga ggtcgatttt aactgccagt gaggaaaacc gaacattgcc atcagctgtc
    65221 tccttaagga ggttaacaag cagggcgcat cagcgcgtga taactgcgat cgaaccacac
    65281 cagcccgtag ggtgtggcgt gacgatgaat cgcttcgatg gcgcaaaaca aaatgtcgtg
    65341 ggtgccgacg ctcaccacct ggctgatacg gcagtcaaac gaaaccagtg cctcttccag
    65401 ttgcgggcat ccggtcacac cagtctgcca gcgggcggcg gcaaagcggt gttccatggg
    65461 cgttttgccg ccaaaaaggt ttgaaagcga ctcctgcccg gcgctaagtg tatttacaca
    65521 cagcgttcga ttttcattga ataccggcca gacggacgcc ccacgattca ggcacaccag
    65581 taatgtgggc ggcgtgtcgg tcacgctgca gacggcgctg gcggtgaacc cggcgcgccc
    65641 ggctggaccg tccgtagtga tgatattgac cgccgcaccc atacaggaca tcgcatcgcg
    65701 aaaggtttgt tgatcgacaa tgttcattgt ttgctcctta caacagcccg caggcttctt
    65761 caaaggacag acgtggcagg cgcgcaaaaa gtttgctgga atcgccatag ccgatattaa
    65821 tcagcagatt gctcttcagc gtgctgcccg caaaaaaggc atcgtcaacg tattgacggt
    65881 caaagcccga catcgggccg gtatccagcc ccagcgcacg gcaggcgacg atcagatagg
    65941 ccgcctgcat ggaactgttg cgaaacgctg tttcttcggc aagttgtgga ctggaggtaa
    66001 accaactgcg ggcatcgccg tgaggaaaca gctgtggtaa ccgttcataa aattcactgt
    66061 cccaggcgac gatagcggtg acgggcgcgg tcagggtttt ttgcagattg ccgctggaaa
    66121 gcgccgggcg cagacgttct tttccttctg cagtgcggat aaacacgatc cgtgccggag
    66181 aacagttagc agatgtcggc ccccatttca tcagggcata aatctcccgt aacgtctcat
    66241 cgctgacggg gggctcccgc cagccgttgt gagtgcgggc atcggtgaac agggtgctaa
    66301 gcgcacctgg gctaacggct tcgttcatag caattcctta cagagcggct tcacggtgat
    66361 gtaacaggct ggcaagcccg ttgagtaaca gagcattaaa cgtttcggga tcggtcacgt
    66421 tgcaagcgtg tccgccatag cgcatcacca ttttctggct atcgggcagg gcggcatgaa
    66481 gttcactgga acatgctgtt ggcaccagca gatcatcact ggcgcagatg atttgcaccg
    66541 ggcagcggat acgatccgca tggtgactaa agtcagcgcg tttgagggcg ttaagtcgac
    66601 gcagtaaatt atttttgcct tgaaaatgcg ccagtgccag cgcgtcttct gcctcaaggc
    66661 gaggtgcgcg ggccgccatc cagtcggcgg gatagaggaa caacggctgc gcttccaccc
    66721 atgcctgcgc gccgccgcta tgtagtaact gttcgcgaac ctgaaaacag cggcgcgtat
    66781 gggcgtttat tcgtagccag ccgttaacgc tgaccagcac agttaccgac gcgggataat
    66841 ccagcgccag ctgcattccc accagcgcac cgagcgcatg gccgaccacc gcgtaacgct
    66901 caatccctgc ggctaccagc gcctgatgca gttccgctgc catctgggcg atactgtaat
    66961 cttctgccag cgtgtcggga ttattgccgg tgccgcgctg gtcgtaacag actacctgat
    67021 actcctgatc cagcaccgcc agttgcggta accagtaact gccgctaccg ccaagacccg
    67081 aaatcaacac cactacgggc gcatcagcat aagggggagg tgagagtgaa agtttcatcg
    67141 cggcctcact tggcgatatg cgcaattgtg gcgatttcca ccagcgcgtc aggttttacc
    67201 agtccgcact gaatgcagaa tcgcgccggt ttgtcacccg ggaaaaactc ggcgtagatt
    67261 tcgttaatcg cggcgtaatt tttccagtcg gtaataaaga tgctgttgaa ggtcacatcc
    67321 gccatcgtgc cacccgccgt ctcgatgacc ttgcggatgg tttccagaac gtggcgggtt
    67381 tgcgcctttg ggtcatcggc aaacagcacg ttgttatgtt gatcaaaagc cagcgtaccg
    67441 gagacataca ccacgccatc agccagcgtg ccgggaacga agggggccag cggtgcgctg
    67501 ctgccagcag gaataattac ggattttggc atcgttaaac tccttaagcg atacgagcaa
    67561 aggacgtgga agaaagcgcg tcgcagaacg tttcgacgtc gctgacccag ccaaaaaagg
    67621 tttcgatatt gaacaacgcg gctttctgtg caaattccgg ccccgcctgg tgtgttgcgt
    67681 cttccagcac cacgccgaaa tactccagaa aaaagccgtc gcgtagcgtc gattcgacgc
    67741 agacgttggt agcgataccg gtgaaaacca gatggcgtat tccacggctg cgcaaaatgc
    67801 tgtccagcgg cgtattgaag aaaccgctgt agcgcggctt cggcagcaca atatcgccag
    67861 gctgcggcat cagttcatcc accagttgat aatcccagga gcctttcgcc agcaatttcc
    67921 cctgcagctg cggctgatta cgcatggttt tcagggcgtt cgatttatga aaatttggtg
    67981 agccggggcc gccagcctcg acatactgtt catcccagcc attttgaaac cagatgatca
    68041 gcatccctgc cgttcgcgct gcggtcacgg cggtttgaat gttggcaatg accgggcgag
    68101 tggttgagac atcaaacccg gcgagatcta agtagccgcc tggcgtggca taggcatttt
    68161 gcatatccac cacgatcagc gcagtttgct gcggatcgaa ggtaatggct tccggtcgtg
    68221 cggttaaggt cgtcatcatg ccacctcctg agtcagcgca gggagatggg cgcggcactg
    68281 catcagtggt tgaatgcgct cgccgaagtt ttcgattccc gacagaaaat catcgaaggt
    68341 caacagcacg ccttcggcac caggcacgct tgcgacttca tctaacatgc gcgcgacact
    68401 ggcgtaagaa ccgactaacg tccccatatt gatgtttacc gccgaagtgg gatcggccat
    68461 ctgacggacg ttggtgtctg taccggagcg ggtatctttc tgactttgtt cggttagcca
    68521 gcttaacgcc tcttcatccg cgcccgcttt gtagtgttcc catttggcgc gagcggcatt
    68581 gtcggtttcg tcggcaatca ccataaacaa cacataagag ccaacgtcgc gtccggtttg
    68641 ctctgcggcc tgtttcatcc gcgcagcggt cggggcgaaa gccgtgggtg tatttacgcc
    68701 tttgccgaaa cagaagttaa aatcggcata ccgggcggag aacgccatgc cagcgtcgct
    68761 ttgcccggcg cagatcactt tcatggggac actcggttgc ggactgacgc gacaatcatc
    68821 catagtgaaa aaatcgcctt taaaatcgct tttccccgag ccccacaggt cgcgcagcac
    68881 ctgaacatat tcggtgagat agtcgtaacg acgggagaaa tagtcatcgc caggccagat
    68941 ccccatctgc tcatactcgg gcttctgcca gccagtcacg aggttgacgc caaaacgccc
    69001 gccagagatg gagtcgatgg ttgcggccat acgggcgacg attgccggag gtaacgttaa
    69061 ggtggcagct gtcgcgtata tctgaatgcg tgaggtgacg gctgccagcc ccgccatcaa
    69121 ggtgaacgac tcaaggttgt gatcccagaa ctcagttttg ccgccaaagc cacgcagttt
    69181 gatcatcgac agggcgaaat cgaaatggta gtgctccgct ttttgcacaa tggctttatt
    69241 cagttcaaat gtcggcatgt actgcggcgc gtgggtcgaa atgagccagc cgttgttgcc
    69301 aataggtacg aatacgccaa ttttcatcat caacctctct tcgtctcgta aagtgaaagt
    69361 cagacggggc gctgcatcct gcatatcctt ttcagccgcg taatggcttg tttgcaaagc
    69421 ggatgccagt ttttaaaaag tgaatgttat taatgtgtta atgttaagtt gtctaaaatg
    69481 cctaaaaaaa agtggactaa acggtcaaaa cagttgcaca taaaacatgc attcgtgcgc
    69541 gatgagagtg cagaaggtcg aggccgggcg ggggttttgc tatcctgttg ccaatcaaca
    69601 agaggggaga gcgcatgacg caaggcgcag tgaaaacaac gggtaagcgt tcgcgcgcag
    69661 taagcgcgaa gaaaaaagcg attcttagcg cagcactgga cactttttca caattcggtt
    69721 ttcacggcac aaggctggag cagatcgcgg agttggcggg tgtttcaaaa accaatctgc
    69781 tgtattactt tccgtcaaaa gaggcgctgt atatagccgt actgcggcag attctcgata
    69841 tctggctggc accgttaaaa gcgtttcgag aagatttcgc cccgctggcg gcgatcaaag
    69901 aatacatccg tctgaagctg gaagtttccc gcgattatcc gcaggcttcg cgcctgttct
    69961 gtatggagat gctggcaggt gcaccgctgt taatggatga actgacgggc gatttgaagg
    70021 cattaattga tgagaaatcg gcgctcattg ccggttgggt taaaagcggc aaactcgcgc
    70081 cgattgaccc gcagcatttg atttttatga tttgggcttc cactcaacat tacgccgatt
    70141 ttgcccctca ggtggaggcg gtgacaggcg cgacgttgcg cgatgaggtg tttttcaatc
    70201 aaacggttga aaacgtgcag cggattatta ttgaggggat tcgaccacgt taaagatgcc
    70261 ggaggaggtt gtaacatcct ccggctacct gtttaaccga tcgtcattaa actggcgtta
    70321 ccgccagcag cagcggtatt cacactcagc gaacgctcga tatacagccg ttccagaagg
    70381 atattgcttt cgccacgggc aaaaccctgc accgaaacaa ttgcaccatc ccgtgcggca
    70441 acggcttcac acaatgcgcg aagctgatcc gaatcaccgt ggaagatcac cgcatcaaat
    70501 ggttgagcgg ttatattttc cgctttcgcc agttgaatac gttcgctgac tgccgatggc
    70561 aatgccttca ctaactgacg atgcagcgca tcatccggcc acagcacctg gctacccacc
    70621 gccagcacgg cggcgagctg agtcagcgca tcctgttcat catccgcaat acacaacacg
    70681 cgctcacgcg gcagcagcgt ccaggtgttg cgttcacccg tcggccctgg caacaatcgt
    70741 tgtgttcctg cctgcgccaa ctcgccatat tgcgtacata acgcctgcaa ttctggacgg
    70801 tttgccgccc attcccgcag tgcatttagc ggctgagtca atgcggcttt caactgcgca
    70861 tcgaccggat actccgcatc ctgacgcgcg agcgtcaccg ccagcgcact ttccgggcga
    70921 ttcgccagca gacggtagag atagagcgga ccgcctgctt tcggcccggt accggacaac
    70981 ccttcgccgc cgaacggttg caccccaacc accgcgccca ccatattacg gttaacgtac
    71041 agattaccga cctgggccga gccagtgacc tgggcaatgg tttcatcaat acgcgtatga
    71101 acgccaagcg tcagaccata gccggaagcg ttaatctgct cgatcagctc tgggagctgg
    71161 ttacggttgt aacgcaccac atgcagcacc ggaccaaaga cctctttttg taattcggca
    71221 aagtcatcca gttcgatcag cgtcggagcg acaaaggtgc cgctttgcca ttcacgggca
    71281 tcttcgctgt tttcccgcac cgcctggaac acctggcggc ctttactacg catggtctga
    71341 atatgacgct cgatattggc tttcgcttcg ctatcaatca ccggaccgat atcggtggtc
    71401 aggcgacccg gattacccat ccggcattcg gccattgcgc cgcgcagcat tttcagcgtg
    71461 tggtcggcaa tctcatcttg caggcacagc acgcgcagtg ccgaacaacg ctgacctgca
    71521 ctgtcgaacg ccgaggccag cacatctatt acgacttgtt cggtcagtgc tgaagaatcg
    71581 acaatcatcg cgttcatgcc gccggtttcc gcaatgagcg gaattgggcg accctgagcg
    71641 tccaggcggc tggcgatatt gcgttgcagt aacgtagcga cttcggttga accggtaaac
    71701 atcaccccgc gcacgcgttc atcacccgtc agttgcgcgc ccacggtttc accccgacct
    71761 ggcagcaatt gcaccacgcc tggcggtaca cccgcttcca gcaaaatggc gatcccttgc
    71821 gcggcaatca gcggcgtttg ttctgccggt tttgccagca cgctgttacc tgccgccagt
    71881 gcggcagcga tctgcccggt gaaaatagcc agcgggaagt tccacggact gatacatacc
    71941 acaggcccta atggacggtg ggtttcgtta gcgaaatcat cccgcacctg tccggcatag
    72001 tagtggagaa aatcgaccgc ttcgcgcact tcggcaatgg cgttactgaa ggtttttccg
    72061 gcttcacgca ccagaatacc aatcagttgc tgcatctggc tttccattag cacggcagcg
    72121 cgatgcaaaa tcgccgcgcg ttcagccgga ggagtggcaa accagattgg cgcgttatta
    72181 accgcacttt ccagcgcctg ttctacttca cgcggcgtgg cttcacgcac atagcccaca
    72241 atatctttcg gttccgcagg gttaataacg ggcgacatct caccagccgc gaccgattgt
    72301 tccagcattg gcaaggcctg ccatttttgc agtgcactat tgagcagggc agaggagagc
    72361 gaggccagac ggtgttcgtt agcgagatcc agccctgccg agttgtcgcg cccgtgaccg
    72421 taaagatcgc gcggcagggg aattttcgga tgcggtaatc cagtttgccc ttcctgttgc
    72481 gccagttttt ctacagcagt gactggatcg gcgaccagtt catccagtgg caaagaggtg
    72541 tcggcaatac ggttaacaaa cgaggtgtta gcaccgtttt ccagcaggcg gcgcaccaga
    72601 tatgccagca gcgtttcatg tgtaccgacc ggagcataaa tacgacacgg acggttaagt
    72661 ttgccgtcgg caactttccc ggtgacctgc tcatacagtg gctcgcccat accatgcagg
    72721 cactggaact cgtactgacc cgggtagtag ttttgacccg ccagttgata aattgccgcc
    72781 agcgtatggg cgttgtgcgt cgcgaactgc gggtagatta gatttggcac cgccagcagt
    72841 tttttcgcac aggcaagata agaaacgtcg gtatacacct tgcgggtata aaccggataa
    72901 ccttcaaggc cgtccatctg tgcacgcttg atttcactat cccagtacgc gcctttcacc
    72961 aggcgaatca tcagacggcg acggctgcgg gtggcgagat caatcaggta atcgatcacc
    73021 aacgggcagc gtttttgata agcctgaata acaaaaccga tgccgttcca gcctgccagt
    73081 tcaggctcga aacagagttt ttccagcaga tcgagggaga tctccagacg atcggcctct
    73141 tcggcatcga tgttaatacc aatatcgtac tgacgcgcca gcagggtgag tgatttcaga
    73201 cgcgggtaaa gctcttccat tacccggtca tactgggcgc ggctataacg cggatgtagc
    73261 gccgacagct tgattgaaat gcccggacct tcatagatgc cacgaccgtt agacgcttta
    73321 ccaatggcgt gaatcgcctg ctgataggaa accatatacg cttgtgcatc tgcggcagtc
    73381 agtgcggctt cgcccagcat atcgtaagag taacggaaac ctttctcttc cagtttgcgg
    73441 gcattggcta acgcttccgc gatggtttcg cccgtgacga actgctcgcc catcaggcgc
    73501 atcgccatat ccacaccttt gcggatcagc ggttcaccgc tcttaccgat aatgcggttc
    73561 agcgagcggg agaggctggc ttcgttatgg gtggaaacca gtttgccggt aaacagcagc
    73621 ccccaggtag cggcattaac aaacagggac gggctacgac caatgtgtga ctgccagtta
    73681 ccgttactga ttttgtcgcg aattaacgcg tcacgggtgg ctttgtcggg aatacgcaac
    73741 aacgcttccg ccagacacat cagcgccacg ccttcctgcg atgacaacga aaactcctgc
    73801 aataacccct gaaccatacc tgcgcgacca ctggcgtttt tttgattacg cagtttatcg
    73861 gccagttgat acgccagttt gtgcgcctgt tcagctactg gctgcggcag gcgagcttgt
    73921 tccagcagca tagaaaccgc ttcggtttcc gggcggcgat aggcggcggt gatggcggcg
    73981 cgggaaaccg actggggcaa tatttgctcg gcaaagtcga ggaaaggctg gtgtggttcc
    74041 tctgccggag tcggtgcttc atcgctctca ttggccgcgc cagaaagcag cgctggtagc
    74101 tccgggagag tatcgctgtt ttccagttgt tcaagataag aaaaaatcgc ctgtttaatt
    74161 aaccagtgtg gtgtgcgatc gatacgtgtc gcggcagact taatacgctc acgcgtcgcg
    74221 tcgtccagct taacccccat ggtggtggtt cccatgccat tactcctgtt gttctgaaag
    74281 gtgcaactta acgttatcgt gaaatatcca tgatgttgca actttgtgca accgtgttaa
    74341 atgtgacctg cgtagcaagc ttaaaaatga atgaaatgtt aataaacgaa atcgatatga
    74401 cagggattaa aaaaataact cagacttttt ctctgcggca gttaacattt ttgaaaggtg
    74461 caaccgcaaa aaatgtgaga gagtgcaacc tgatgaaaaa tagtgtcgct gagcactaaa
    74521 atttaatgta aatggtgtgt taaatcgatt gtgaataacc agcgcttccg gcaggatacg
    74581 gtcgccctgg taaaacataa actctgttac cccgttccgg tggcagatat aacggcaagt
    74641 ttcgacattg ccgataataa ttttttggag actttagatg gctattagca caccgatgtt
    74701 ggtgacattt tgtgtctata tctttggcat gatattgatt gggtttatcg cctggcgatc
    74761 aacgaaaaac ttcgacgact atattctggg cggtcgtagt cttgggccat tcgtgacggc
    74821 attatcggcg ggtgcgtcgg atatgagcgg ctggctgtta atggggttgc caggcgctgt
    74881 ttttctttcc gggatttccg aaagctggat cgccattggt ctgacattag gcgcgtggat
    74941 caactggaag ctggtggccg ggcggttgcg tgtgcatacc gaatacaaca ataacgcctt
    75001 aacattgccg gattatttca ccgggcgctt tgaagataaa agccgcattt tgcgcattat
    75061 ctccgcgctg gttattttgc tgttcttcac catttattgc gcttcgggca ttgtggcagg
    75121 cgcgcgtctg tttgaaagta cctttggcat gagctacgaa acggctctgt gggcgggggc
    75181 tgcggcgacg atcctttaca cctttattgg cggtttcctc gcggtgagct ggactgacac
    75241 tgtacaggcc agcctgatga tttttgccct gatcctgacg ccggttatcg tgatcattag
    75301 cgtcggtggg tttggcgact cgctggaagt aatcaaacaa aagagcatcg aaaacgtgga
    75361 tatgctcaaa gggttgaatt ttgtcgccat tatctcgctg atggggtggg ggctgggtta
    75421 cttcgggcag ccgcacatcc tggcgcgttt tatggcggca gattctcacc acagcattgt
    75481 ccatgcgcgt cgtatcagta tgacctggat gatcctctgc ctggcagggg cggtggctgt
    75541 cggcttcttt ggtatcgctt actttaacga gcacccggcg gtagcaggtg cggtaaacca
    75601 gaacgctgaa cgcgtgttta ttgaactggc gcaaattctg tttaacccgt ggattgccgg
    75661 gattttgctg tcggcgattc tggcggcggt aatgtcaacg ttaagttgcc agctgctggt
    75721 atgttccagt gcgattaccg aagatttata caaagcgttt ctgcgtaaac aggccagcca
    75781 gaaagagctg gtgtgggtag ggcgagtgat ggtgctggtg gtggcgctgg tggcgattgc
    75841 gctggcggca aacccggaaa accgcgtgct gggcttagtg agctacgcgt gggcaggctt
    75901 tggcgcagcg tttggtccgg tggtgctgtt ctcggtgatg tggtcacgca tgacgcgcaa
    75961 cggtgcgctg gcggggatga tcatcggtgc gctgacggtt atcgtctgga aacagttcgg
    76021 ctggctgggt ctgtacgaaa ttattccggg ctttatcttc ggcagtatcg ggattgtagt
    76081 gtttagtttg ctgggtaaag cgccgtcagc ggcgatgcaa aaacgctttg ccgaggccga
    76141 tgcgcactat cattcggctc cgccgtcacg gttgcaggaa gggtaaggga aggcgtttta
    76201 ccggaaattt cacgtcataa caggaagcag gcagagggca atcagtgatt gccctctgcg
    76261 ttttttctca aaatattttt acatcttctc atcattatca ctacattcca cttgtatttt
    76321 tcttcatcgt aatgataatc gctatcactg cgatttactt ttctttgcat agattgactc
    76381 agaaaaacgt ttaagggtgg gtggcatgtt tgttccgttt ctcattatgt tgcgcgaagg
    76441 acttgaagcc gcgctgattg tcagtttgat tgccagctat cttaagcgta cccagcgagg
    76501 ccgatggatt ggtgtaatgt ggattggcgt gttgcttgcc gctgcgttgt gcctgggctt
    76561 gggtatcttc attaacgaaa ccaccggcga atttccgcaa aaagaacagg aactgtttga
    76621 aggtatcgtg gcggtgatcg ccgtggtgat ccttacctgg atggttttct ggatgcgcaa
    76681 agtgtcgcgc aacgtcaaag tacaactgga gcaggcagtc gatagcgcat tgcagcgtgg
    76741 aaatcatcat ggctgggcgc tggtgatgat ggtctttttt gccgttgcaa gggaggggct
    76801 ggagtcggtc tttttcctgc tggcggcgtt tcaacaagat gtcgggatct ggccgccgct
    76861 gggtgcaatg ctcggtcttg ctacagccgt ggtgctcggc ttcctgctct actggggcgg
    76921 tattcgcctc aatcttggtg cattttttaa atggaccagc ctgtttattc tcttcgtcgc
    76981 cgcagggctg gcagctggtg ctattcgcgc atttcatgaa gccggattgt ggaaccactt
    77041 tcaggaaatc gccttcgata tgagcgcggt gctctcaact cactcgctgt ttggcacact
    77101 gatggaaggg atttttggct atcaggaagc accgagcgtc agcgaagtcg ccgtctggtt
    77161 tatttatctc atcccggcgc tggtggcatt tgttctgcca ccacgcgcag gggcgactgc
    77221 gtcccgctcc gtgtagcaaa tacgacgcaa actcttgctt agttacaaca tactttaaag
    77281 ggatagtctc gtcatgacca ttaacttccg ccgtaacgca ttgcagttga gcgtggctgc
    77341 gctgttctct tctgctttta tggctaacgc cgctgatatt ccgcaagtca aagtgaccgt
    77401 gacggataag cagtgcgagc cgatgaccat tacggttaac gccgggaaaa cacagttcat
    77461 tattcagaac cacagccaga aggcgctgga gtgggagatc ctcaaaggcg tgatggtggt
    77521 ggaagagcgg gaaaatatcg cccctggttt tagccagaaa atgacggcga atttacagcc
    77581 tggcgaatac gatatgacct gcggtctgct gactaacccg aaagggaagt tgatcgtcaa
    77641 aggtgaagca acggcggatg cggcgcaaag tgatgcgctg ttaagtcttg gtggtgcaat
    77701 tactgcatat aaagcgtatg tcatggcgga aaccacgcaa ctggtgaccg acaccaaagc
    77761 ctttaccgac gctattaaag caggcgatat cgaaaaagcg aaagcactgt atgcgccgac
    77821 gcgccagcac tatgagcgca tcgaaccgat tgctgaactg ttctccgatc tggatggcag
    77881 cattgacgcc cgtgaagatg attacgagca aaaatccgcc gatccgaaat tcaccggttt
    77941 ccaccgtctg gaaaaagcat tgtttggcga taacaccacc aaagggatgg ataagtatgc
    78001 tgaccagctt tataccgatg tggtcgattt gcaaaaacgc atcagtgaac tggctttccc
    78061 tccttcaaaa gtggtcggcg gtgcagccgg actgattgag gaagtggcag ccagcaaaat
    78121 cagcggtgaa gaagatcgct acagccacac cgatctgtgg gatttccagg ctaacgttga
    78181 aggctcgcag aaaattgtcg atctgctgcg tccacaacta caaaaagcga atccggaact
    78241 gttggcaaaa gtcgatgcca acttcaaaaa ggtcgatacc attctggcga aataccgtac
    78301 taaagacggt tttgaaaatt acgacaaact gaccgatgct gaccggaatg cactgaaagg
    78361 accgattact gcgctggcgg aagatctggc gcaacttcgc ggtgtgctgg ggctggacta
    78421 agcgttatgc agtatgaaga tgaaaacggc gtgaatgaac cgtcacgccg acgtttactg
    78481 aaagggattg gtgcgctggc gctggcggga agttgtccgg tcgctcatgc acaaaaaacg
    78541 caaagtgcgc cggggacgct ttcaccggat gcacgcaatg agaaacagcc gttttatggt
    78601 gagcatcagg cagggatcct gacaccacaa caggctgcaa tgatgctggt ggcgtttgat
    78661 gtgcttgcca gcgataaagc cgatcttgaa cggttgtttc gcttgttgac tcagcgtttt
    78721 gcttttctga ctcagggcgg agcggcacca gaaacgccaa atccgcgcct gccaccactc
    78781 gattccggca ttcttggcgg ctacattgcg cccgataatc tcaccatcac gttatcggtg
    78841 ggtcactcat tgtttgatga gcgctttggc cttgcgccac agatgccaaa aaagttgcag
    78901 aagatgacgc gtttccctaa cgactcgctg gatgcggcgt tatgtcatgg tgatgtgttg
    78961 ctacaaattt gcgccaacac ccaggacacg gtgatccatg cgctgcgcga tatcatcaaa
    79021 cacacgccgg atttgctcag cgtgcgctgg aagcgggaag ggtttatttc cgaccacgca
    79081 gcgcgtagta aaggcaaaga gacgccgata aatttgctgg gctttaaaga cggcaccgcc
    79141 aatcccgata gccagaatgc gaagttgatg caaaaagtgg tgtgggtgac ggcagatcag
    79201 caggagcctg cgtggacaat cggtggcagc tatcaggcag tacgcttgat tcagtttcga
    79261 gtggaatttt gggacagaac gccgctgaaa gagcagcaga cgatatttgg tcgtgataag
    79321 caaaccggtg cgccgctggg catgctgcat gagcatgatg ttcccgatta cgctagcgat
    79381 ccggaaggga aggtgattgc gctggacagc catatccggc tggcgaatcc ccgcacggcg
    79441 gagagcgagt ccagcctgat gctgcgtcgt ggctacagtt attcgttggg cgtcaccaat
    79501 tccgggcaac tcgatatggg gctactgttt gtctgctacc aacacgatct ggaaaagggc
    79561 ttcctgacag tacaaaaaag gttgaatggc gaggcgctgg aggaatatgt caaacctatc
    79621 ggtggcggct atttctttgc gctgccgggt gtgaaggacg cgaacgatta tctcggaagc
    79681 gcattattgc gggtttaatg tttttaggcg gataaggcgt ttacgccgca ccaggcgttc
    79741 gtacactgat gcgacgcttg cgcgtcttat catgcctaca atcggtgcgg gtcggtaggc
    79801 cggataaggc gttcacgccg catccggcaa gcttttacca gtactgcgaa tcctgctgaa
    79861 agcacacagc ttttttcatc actgtcatca ctctgtcatc tttccagtag aaactaatgt
    79921 cactgaaatg gtgttttata gttaaatata agtgaatata ttgttgcaat gaatgcgaaa
    79981 tctgttgtac ttattaagta gcagcggaag ttcccggcag tgatagtcag tcactatgga
    80041 gatcgcggat ggtaacgtcc tgtactggac atgttttaga taatcagcgc gccaccactc
    80101 gcggagattt ctcctccggt agtcatctcg tcactttgta ttttcaacct catcctttct
    80161 tttcatgtgt taccgacgcc gtaaacggtg cgcgtagccg tttttcggca ttttatccaa
    80221 aagcaaacta tggcttacaa ggaagccaac cctctgatgt tcgtgcgcat aatcgcgctg
    80281 ccaacggcgc gtgtgatgaa tacaaacaac tcaaggtgct ctccatggga agacaaaaag
    80341 cagtgatcaa agctcgtcgc gaggcaaaac gtgtgctgag acgggattcg cgcagccata
    80401 agcagcgtga agaagaatcg gtcacctcgc ttgtgcagat gagcggcgta gaagccattg
    80461 gtatggcccg cgacagtcgc gatacctcgc ccatcctcgc gcgaaatgaa gcgcaattgc
    80521 actatctgaa ggctattgag agtaagcagc tgatatttgc cacgggcgaa gccgggtgcg
    80581 gaaaaacctg gatcagcgca gcaaaagcgg cagaggccct gatacataag gatgtcgaca
    80641 ggattatcgt tacccgtcca gttctgcaag ccgatgaaga tcttggtttc ttacctggtg
    80701 atatcgcaga aaagtttgct ccctattttc gcccggtcta cgacgtgctg gtccgtcgct
    80761 taggggcttc ctttatgcag tactgcctgc gaccggaaat agggaaggtg gaaattgcgc
    80821 cgttcgccta tatgcgtgga cgtacctttg aaaatgcagt cgtcattctt gacgaggcgc
    80881 agaatgtgac tgccgcgcaa atgaaaatgt ttttaacccg cctcggggag aacgtgacgg
    80941 ttatcgtcaa cggtgatatc acgcaatgcg atttgcctcg cggcgtgcgc tccggattaa
    81001 gtgacgcgct ggaacgtttc gaagaggatg aaatggtcgg gatcgtccga ttcggtaaag
    81061 aggattgcgt acgttcggca ctttgccaac gtacgctaca tgcatacagt taagtgtatt
    81121 atcggtgcag agcccgggcg aaccgggctt tgttttgggt gtttataccc ggactagcgt
    81181 tttttctgaa accaccattt ttatttgtcc ctgactggtg aagtgtacgc tcatcctgtg
    81241 gcttttttgt agttgctgat aaagctcatc aggtattgct aagctctctg catattcttg
    81301 cggcgtatat tggtatgctg catgatgctg ctgtttttta aaacgcagct tattgtacaa
    81361 tgcccagacg attaacacga cggcattcgc cactgccagc aaaaaataga actgaagtcg
    81421 gcttctggcc tcgctttgcc agtaataacc cgtcagcaga tcaatggcga ataagaatat
    81481 gaacaaagca aataatgtcc acaagatggt tgtggcaaca taatcaacca gtaaacgtac
    81541 tggcgattgt cgggtcgtaa taattagatt gttcatatta acctctcaga atcccacgat
    81601 cgggacttac ccaacgagcg cgttgttttt taggcatcaa catgacacgt gtaaatgata
    81661 ccaatgtcgt tgccaggctt agcatccaga aaataaccgg aaaccaaata atccagaaaa
    81721 gcgatgaagc cagattgtgc tcatagcgat tctcaattac cagactgaca ataaattgca
    81781 gtaaacataa cgtgcacaat aatattccgg cagtatgtgt cgctgcgata tgtgtcaatt
    81841 caatatttaa cggtacacct gcaagttgga ctgcgtaaat aatgaaaccg accaggcagg
    81901 taaaggccca tattgtcgtc aggcagtatt caaaaaacag tggccacatt cgaaagtttt
    81961 cttttcgcca caaccttgtc atatttttga ggaatacttc tgcaccgccc tgagcccagc
    82021 gcaggcgctg tttccacagc ccttttaacg tttcaggcat taatatccag cacagcgccc
    82081 gtggctcgta aaaaatcgtc cactgattca actgcagctt ccagctaata tcaatatctt
    82141 cggtgatcat atcgtcactc cagtaaccca cttctgccag ggcgctgcga cgaaatgcgg
    82201 caataacacc ggaaacggta aatacgtttc catagatacg ctgggtccgc ttgatcaaac
    82261 caataattga ggaatactcg ccaacctgaa ttttacccac cagggtagaa cgtgtgcgaa
    82321 tacgaggatt accggttacg gcacccacac gtgggttgta caacatcggt tccacaatat
    82381 atgccgctgc atcgcggtct aataacgcgt cgccatcaat gcacaccaga tattcacttt
    82441 tcgccgcggc agctccggtt ttaagcgcaa tggctttccc ctggttttgc gccagatgaa
    82501 tgacccgcag acggggaatt tgtgcagcca tgtgatccag gatggcacgg gttttgtctg
    82561 ttgaaccatc atttacggca ataacttcaa tgttctcata acgctgtgct aaagcggcat
    82621 gtatagtttc ctcaacgttt ttctcctcat taaaacaggg aatgataatg gagatagatg
    82681 gattatcttt caactggggg gctggtgcgt tttctcccca cggccagtgg cgttcacgat
    82741 agacccagaa atagacgccg ccaataatcc acataatgga cataaaaaac ggccagaaga
    82801 aaacgaacct catcatcagt tcaccagagt gaaagtacgc tacgcataga ggtatgcata
    82861 acaccagaca taatataaaa aacgatacga tgcgattaat catttttcgg ataccaggct
    82921 gttgaaaact caggacgaat aacgtctatt tcaggttggt tatgcagaaa attgtcggga
    82981 taataaccat aatttttcac tccattcagt tgtaataggc tcatccagtg agcgagttgt
    83041 tgtgaagaaa tagcctgatg ctgaccattt ttctgccagt tttgtgcctg taattctaaa
    83101 attgatttgt ctttagcctg cgggatgctt ttcatctgat tggtcaattg tgttaaccat
    83161 tgatcagccg atttttctgt gacgccttcc atataaggca tagccataat agcggtccag
    83221 tcatagcttt ttaggaaatc agcataattc tgtgcaaacc aggcttcact ttcaggttgt
    83281 attaccggaa gtgcaaaaat atttcgtgca gttttaacat gtggaccacg aatggctttt
    83341 acgcgcgcac taagttctaa agtgaagtca gttaacgcac gacttttaaa gcgggtccac
    83401 tgtttaaatt gctccgggtt ttgtcgaatt tcgctcagac tcccgctaaa gccagcttgc
    83461 tgataagccg cgatagccgc tgcactggca tcttcataat ctgaaagcaa agcatcatcg
    83521 tggaacaata tgccatcaaa agcagcatgt ccggcaagat catcatataa catgccaact
    83581 tgtgctctga ccctgtcatc gaaaggagag agacggcggt attgttcagg atgaatttgt
    83641 gcttttttct ccccagttgg taagtatttt actcgcgtta atgtgggatc taaatcccag
    83701 cttaataccg gcatccacgc atagatgttt acacctgagc gggtacgcaa ttgccaggca
    83761 acccgactaa aaatatctgc tttcattggt agcaaacgat ttggaaacca gacctcttta
    83821 accagcccat caccatcggg atcagcaaat gcttgtaaat acacggtaga tatttgcata
    83881 tcttttaccc gctgaattag cacatcaata ttgcgatcca tttgctggcg gttttcgtca
    83941 taaatgtaat caagatcgat atgcattacc cgttgtagtg gtttttcctg tacggtaata
    84001 atttgctggg caaactcttt taatgaggga ttattggcga ttaatactcg cggaatggaa
    84061 tctaattgcg acgcatttgc caaacctgat tcaagggtga agaacatgtc ataaccgagt
    84121 ttttttaatt ccgcgattgc tatgccattc gcttcgccat aaggccaaac aaaaacgtgt
    84181 ggatttacct cagcctttgt acgcaggtat tccgtcattt ttacagcatc cagacgaatt
    84241 ctttcccggt attctgctgc ggtttcatac cgtgcatggt cagtaaaata tgcacgattt
    84301 acatatgcag gtaataagct gccggtggca ttagcctgaa taccgtagtg agaatcccat
    84361 gtatgagaag cgaccgcaac gagccgggaa cgcgcaactt ctcgcacttg ttgccacgtg
    84421 gcaaaatatt ctcgactgac catctcatca ccaaatttca cttgtttatc cgctggagta
    84481 tcgacccaac tgccgacggg agcccataca gccggccact ggaaggcctg aagaattggg
    84541 aagacacggg tataaaaact ctggtagccg tcatcaaaag tcagcactac tgctttttcc
    84601 ggtagcggtt ttcctccttg atgtgcttca cgaatttgag caatactgac cggttgataa
    84661 ccgttctcgc gcagccaggc aaattgttca cgcagtgctg atgtccgcac tgacataaaa
    84721 cgctggtcgg cagcttcgtc ttcaacgtta tgccatgaaa tcgccacaaa accattatgc
    84781 ggccacggtt gctcggcgag taaagattcg cgatcctgtg gcggtataaa tgatgttctt
    84841 gactggctaa tgcacgcagt gagcataatt atactcacca gcatcaggat atatttattt
    84901 ccattacgta acatatttat ccttaaaatc tgaatgtcat atcgaattca acgtataagt
    84961 tgtgttctct gtcaccgtca taaggtcgtt tttcccagcg tagcgttgcg ccagcatcaa
    85021 tcacgtcatt ccagctaata cgttgcccgt agccgagttg ggtgacgaca tccgtgccat
    85081 aatgtttttg ccaggaggca ccaacacctg cgctgaagat ttgctcccag ctattttcat
    85141 agcttcgcca taacaaatga cttgcctcaa atgccggaac aatatcgaac gtttttatag
    85201 ggttgtagta tggagtgtcg tgttctgtat tttgttcgta atacagattg ggtaggaaat
    85261 cgacaatcaa atatggtgaa gaccagatgc gttcctgacc ctcaagtgag acttcctgac
    85321 gctggttact gtcggaaaaa tcagtgaaag cccaggagac accgtactta cgccgctcat
    85381 tttgatacca gcgaacataa gcctgagcac tgttgcctgt aacaccattt ttcattgccc
    85441 gtaatggaac gcggtgagag aggcgttcca gttgcgaacc aatacgccag ttatcattaa
    85501 aatcatacca gccagacagg cgcgcgccgg gtttatgctc atgattgaaa acgcgttcag
    85561 cgtactccgc ctcgagccag atattacgtg accgccactc aacacccgcc agccagtcgc
    85621 gaacaatccc ttttccttcg ctaaattgtc catcggcata accgaatcca gcaaaaccgc
    85681 gccagttatc cttcagcggt ggtgaataaa cgatggtggt taagtcgaca tcatgtttac
    85741 cactatccgg gccttcggca tcaattcctg ttgaaccagc tatacgaagc tctgcaagat
    85801 tatgtacatc aaccgcacgt tttaaacgta caacgccggg atcttgcggt tcacgttcga
    85861 caacatcgtg cgttaagaca gctgtctgct gccattcttg taacgttaat gctgtccatg
    85921 cttgttcaac ctccagatta atattacgtg gctcgatcac ttctgctttt tttaattcat
    85981 tttctgctgc acgaggccaa ccgcgggctt gtaacacact cgcgtaatca atgcgcagtc
    86041 cctgatttcc tggtgcgtta taagcaagct ctctggctat catttcagcc tgaggaagat
    86101 cattactata ttttgctacg gttgagagaa acgaatgccc ctgtaaccag gtatcattcg
    86161 ggatgctcgt aggcgtgccc attaaccgaa ggaaaggcgg cgaagtatta atggtatgtt
    86221 gggtgacagt tagcgcgccg ggataatttt cactctccag gtggctgtaa aagagatccg
    86281 caagttcttc atcggataaa tccggggcaa tggtctcctt gtgataaaag agctctgtca
    86341 ttattgactg tgcttttttt ggctgctgtt ctttgagata agccgacgca acccaatatt
    86401 gcgcccaggg cggaataatt tgcctcgtat tttttaaacg ctgatagtga gaaataacgt
    86461 ctttataacg atcgcgagtt aataacgcgc caagatgatc aacctgaata cgctggtact
    86521 gggcagtgcg gtctgagtta tcgtgccaca gaatttctaa tgcagcgtat tgggcgaggg
    86581 cgcgatcggc aatggcataa cgttcacttt cactgcgcgt aggcataaac gacagtctga
    86641 tcagttcggc atgaatatca gcgcgaatat ctggcgttaa attggcatcg tcaatcgcgg
    86701 cagctagttg attattacgt aatgcctgca cgtattctgt gggatattgt tgcttagatg
    86761 cattttcagg taatgactct gtcatcgccc gtaattcatc ctgatgacgc cccgtcagtt
    86821 tatagatata ggcttctgcg agtaaattgg ctttgtccgg cgctccagat ttaagctgct
    86881 taagtttaac cagcgcagta tcatagtgac ccgcatctgc cagggttaaa atttgtcccc
    86941 gttgataatc cttattttgc ggctccagag agagcgcctt ttgccacagt gtaagcgagt
    87001 tttgccattg ttgcaggtta cgataagcga cggcgacagc tgcataacca cgcgctggta
    87061 attgctgatg acggtagcgg ctgtaaacgg taataacctg tttatcttgc ccggcccata
    87121 aggcaatttg taaccagtca gcaatttggt tattactgag tgttgatttt tgtgcaaacc
    87181 atgacaaagc tggctgagta tttcctttgc gagcttcaat aatcaatgca tcataggcgt
    87241 tattaacagc actcttcgcc gcgggactcg ctgctaaaaa tgcggcagta agaagtttca
    87301 aagcccattt ggttttcggg cacctttttc tgctacttga atacatcctg tattactcca
    87361 tgtattgcca aaatctctct ctatatctaa ttacaggtaa ctgaaaagaa agatattttt
    87421 gcatctcata atccgttatt aaacgcggaa gagacacgtg aattgttgat gatgagaaga
    87481 agaaatgatg agcagattgt ccatataaaa tccttttctc gcccgaaaat ccattccaat
//
LOCUS       DAZFRE010000005        87225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFRE010000005 DAZFRE010000000
VERSION     DAZFRE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.005"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(73..288)
                     /locus_tag="ACHKUI_000414"
     CDS             complement(73..288)
                     /locus_tag="ACHKUI_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6596681.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKNFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
     gene            complement(351..1823)
                     /gene="der"
                     /locus_tag="ACHKUI_000415"
     CDS             complement(351..1823)
                     /gene="der"
                     /locus_tag="ACHKUI_000415"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6596682.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1941..3119)
                     /gene="bamB"
                     /locus_tag="ACHKUI_000416"
     CDS             complement(1941..3119)
                     /gene="bamB"
                     /locus_tag="ACHKUI_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6596683.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3130..3750)
                     /gene="yfgM"
                     /locus_tag="ACHKUI_000417"
     CDS             complement(3130..3750)
                     /gene="yfgM"
                     /locus_tag="ACHKUI_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6596684.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3768..5042)
                     /gene="hisS"
                     /locus_tag="ACHKUI_000418"
     CDS             complement(3768..5042)
                     /gene="hisS"
                     /locus_tag="ACHKUI_000418"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6596685.1"
                     /translation="MAKNIQAIRGMNDYLPGETVIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5153..6271)
                     /gene="ispG"
                     /locus_tag="ACHKUI_000419"
                     /gene_synonym="gcpE"
     CDS             complement(5153..6271)
                     /gene="ispG"
                     /locus_tag="ACHKUI_000419"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6596686.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6298..7305)
                     /gene="rodZ"
                     /locus_tag="ACHKUI_000420"
     CDS             complement(6298..7305)
                     /gene="rodZ"
                     /locus_tag="ACHKUI_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6596687.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7590..8744)
                     /gene="rlmN"
                     /locus_tag="ACHKUI_000421"
     CDS             complement(7590..8744)
                     /gene="rlmN"
                     /locus_tag="ACHKUI_000421"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6596688.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8894..9325)
                     /gene="ndk"
                     /locus_tag="ACHKUI_000422"
     CDS             complement(8894..9325)
                     /gene="ndk"
                     /locus_tag="ACHKUI_000422"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6596689.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9474..11786)
                     /gene="pbpC"
                     /locus_tag="ACHKUI_000423"
     CDS             complement(9474..11786)
                     /gene="pbpC"
                     /locus_tag="ACHKUI_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6596690.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11787..16748)
                     /gene="yfhM"
                     /locus_tag="ACHKUI_000424"
     CDS             complement(11787..16748)
                     /gene="yfhM"
                     /locus_tag="ACHKUI_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6596691.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSHNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     SSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPVKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            16955..17800
                     /gene="sseA"
                     /locus_tag="ACHKUI_000425"
     CDS             16955..17800
                     /gene="sseA"
                     /locus_tag="ACHKUI_000425"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6596692.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVTNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            18136..18261
                     /gene="timP"
                     /locus_tag="ACHKUI_000426"
     CDS             18136..18261
                     /gene="timP"
                     /locus_tag="ACHKUI_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6596693.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVVNNLQNSK"
     gene            complement(18293..19069)
                     /gene="sseB"
                     /locus_tag="ACHKUI_000427"
     CDS             complement(18293..19069)
                     /gene="sseB"
                     /locus_tag="ACHKUI_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6596694.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19212..20495)
                     /gene="pepB"
                     /locus_tag="ACHKUI_000428"
     CDS             complement(19212..20495)
                     /gene="pepB"
                     /locus_tag="ACHKUI_000428"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6596695.1"
                     /translation="MTEAMKITLSPQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20554..20754)
                     /gene="iscX"
                     /locus_tag="ACHKUI_000429"
     CDS             complement(20554..20754)
                     /gene="iscX"
                     /locus_tag="ACHKUI_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6596696.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20766..21101)
                     /gene="fdx"
                     /locus_tag="ACHKUI_000430"
     CDS             complement(20766..21101)
                     /gene="fdx"
                     /locus_tag="ACHKUI_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6596697.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21103..22953)
                     /gene="hscA"
                     /locus_tag="ACHKUI_000431"
     CDS             complement(21103..22953)
                     /gene="hscA"
                     /locus_tag="ACHKUI_000431"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6596698.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22970..23485)
                     /gene="hscB"
                     /locus_tag="ACHKUI_000432"
     CDS             complement(22970..23485)
                     /gene="hscB"
                     /locus_tag="ACHKUI_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6596699.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23581..23904)
                     /gene="iscA"
                     /locus_tag="ACHKUI_000433"
     CDS             complement(23581..23904)
                     /gene="iscA"
                     /locus_tag="ACHKUI_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6596700.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23921..24307)
                     /gene="iscU"
                     /locus_tag="ACHKUI_000434"
     CDS             complement(23921..24307)
                     /gene="iscU"
                     /locus_tag="ACHKUI_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6596701.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24335..25549)
                     /gene="iscS"
                     /locus_tag="ACHKUI_000435"
     CDS             complement(24335..25549)
                     /gene="iscS"
                     /locus_tag="ACHKUI_000435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6596702.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25661..26149)
                     /gene="iscR"
                     /locus_tag="ACHKUI_000436"
     CDS             complement(25661..26149)
                     /gene="iscR"
                     /locus_tag="ACHKUI_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6596703.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26420..27160)
                     /gene="trmJ"
                     /locus_tag="ACHKUI_000437"
     CDS             complement(26420..27160)
                     /gene="trmJ"
                     /locus_tag="ACHKUI_000437"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6596704.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27279..28082
                     /gene="suhB"
                     /locus_tag="ACHKUI_000438"
     CDS             27279..28082
                     /gene="suhB"
                     /locus_tag="ACHKUI_000438"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6596705.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28227..29081
                     /gene="yfhR"
                     /locus_tag="ACHKUI_000439"
     CDS             28227..29081
                     /gene="yfhR"
                     /locus_tag="ACHKUI_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6596706.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTKSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29272..30552
                     /gene="csiE"
                     /locus_tag="ACHKUI_000440"
     CDS             29272..30552
                     /gene="csiE"
                     /locus_tag="ACHKUI_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6596707.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTANTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30544..31683)
                     /gene="hcaT"
                     /locus_tag="ACHKUI_000441"
     CDS             complement(30544..31683)
                     /gene="hcaT"
                     /locus_tag="ACHKUI_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6596708.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            32052..32474
                     /gene="yphA"
                     /locus_tag="ACHKUI_000442"
     CDS             32052..32474
                     /gene="yphA"
                     /locus_tag="ACHKUI_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6596709.1"
                     /translation="MNSLRYFDFGTSRPVLLLIARIAVVLIFIIFGFPKMMGFGGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32634..33506)
                     /gene="yphB"
                     /locus_tag="ACHKUI_000443"
     CDS             complement(32634..33506)
                     /gene="yphB"
                     /locus_tag="ACHKUI_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6596710.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33518..34612)
                     /gene="yphC"
                     /locus_tag="ACHKUI_000444"
     CDS             complement(33518..34612)
                     /gene="yphC"
                     /locus_tag="ACHKUI_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6596711.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLVEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34645..35643)
                     /gene="yphD"
                     /locus_tag="ACHKUI_000445"
     CDS             complement(34645..35643)
                     /gene="yphD"
                     /locus_tag="ACHKUI_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6596712.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35668..37179)
                     /gene="yphE"
                     /locus_tag="ACHKUI_000446"
     CDS             complement(35668..37179)
                     /gene="yphE"
                     /locus_tag="ACHKUI_000446"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6596713.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37202..38185)
                     /gene="yphF"
                     /locus_tag="ACHKUI_000447"
     CDS             complement(37202..38185)
                     /gene="yphF"
                     /locus_tag="ACHKUI_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6596714.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38282..41563)
                     /gene="yphG"
                     /locus_tag="ACHKUI_000448"
     CDS             complement(38282..41563)
                     /gene="yphG"
                     /locus_tag="ACHKUI_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO6596715.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQSQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALVQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATCCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAKEMAKTSIEADFFAVSQPDLL
                     SLYSDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41681..42874
                     /gene="yphH"
                     /locus_tag="ACHKUI_000449"
     CDS             41681..42874
                     /gene="yphH"
                     /locus_tag="ACHKUI_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6596716.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42938..44191)
                     /gene="glyA"
                     /locus_tag="ACHKUI_000450"
     CDS             complement(42938..44191)
                     /gene="glyA"
                     /locus_tag="ACHKUI_000450"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6596717.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44520..45710
                     /gene="hmpA"
                     /locus_tag="ACHKUI_000451"
     CDS             44520..45710
                     /gene="hmpA"
                     /locus_tag="ACHKUI_000451"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6596718.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45755..46093)
                     /gene="glnB"
                     /locus_tag="ACHKUI_000452"
     CDS             complement(45755..46093)
                     /gene="glnB"
                     /locus_tag="ACHKUI_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6596719.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46154..47488)
                     /gene="glrR"
                     /locus_tag="ACHKUI_000453"
     CDS             complement(46154..47488)
                     /gene="glrR"
                     /locus_tag="ACHKUI_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6596720.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGCNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47478..48191)
                     /gene="qseG"
                     /locus_tag="ACHKUI_000454"
     CDS             complement(47478..48191)
                     /gene="qseG"
                     /locus_tag="ACHKUI_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6596721.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48356..49783)
                     /gene="qseE"
                     /locus_tag="ACHKUI_000455"
     CDS             complement(48356..49783)
                     /gene="qseE"
                     /locus_tag="ACHKUI_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6596722.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50359..54246)
                     /gene="purL"
                     /locus_tag="ACHKUI_000456"
                     /gene_synonym="purI"
     CDS             complement(50359..54246)
                     /gene="purL"
                     /locus_tag="ACHKUI_000456"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6596723.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALINANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGSEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLVVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DGAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54504..56060
                     /gene="mltF"
                     /locus_tag="ACHKUI_000457"
     CDS             54504..56060
                     /gene="mltF"
                     /locus_tag="ACHKUI_000457"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6596724.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSVELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(56057..56560)
                     /gene="tadA"
                     /locus_tag="ACHKUI_000458"
     CDS             complement(56057..56560)
                     /gene="tadA"
                     /locus_tag="ACHKUI_000458"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6596725.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(56618..57253)
                     /gene="yfhb"
                     /locus_tag="ACHKUI_000459"
     CDS             complement(56618..57253)
                     /gene="yfhb"
                     /locus_tag="ACHKUI_000459"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6596726.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57462..58310
                     /gene="yfhH"
                     /locus_tag="ACHKUI_000460"
     CDS             57462..58310
                     /gene="yfhH"
                     /locus_tag="ACHKUI_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6596727.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58366..58626
                     /gene="yfhL"
                     /locus_tag="ACHKUI_000461"
     CDS             58366..58626
                     /gene="yfhL"
                     /locus_tag="ACHKUI_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6596728.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(58820..58900)
                     /gene="shoB"
                     /locus_tag="ACHKUI_000462"
     CDS             complement(58820..58900)
                     /gene="shoB"
                     /locus_tag="ACHKUI_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6596729.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(59321..59701)
                     /gene="acpS"
                     /locus_tag="ACHKUI_000463"
     CDS             complement(59321..59701)
                     /gene="acpS"
                     /locus_tag="ACHKUI_000463"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6596730.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(59701..60432)
                     /gene="pdxJ"
                     /locus_tag="ACHKUI_000464"
     CDS             complement(59701..60432)
                     /gene="pdxJ"
                     /locus_tag="ACHKUI_000464"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6596731.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(60444..61172)
                     /gene="recO"
                     /locus_tag="ACHKUI_000465"
     CDS             complement(60444..61172)
                     /gene="recO"
                     /locus_tag="ACHKUI_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6596732.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(61184..62089)
                     /gene="era"
                     /locus_tag="ACHKUI_000466"
     CDS             complement(61184..62089)
                     /gene="era"
                     /locus_tag="ACHKUI_000466"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6596733.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(62086..62766)
                     /gene="rnc"
                     /locus_tag="ACHKUI_000467"
     CDS             complement(62086..62766)
                     /gene="rnc"
                     /locus_tag="ACHKUI_000467"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6596734.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(63038..64012)
                     /gene="lepB"
                     /locus_tag="ACHKUI_000468"
     CDS             complement(63038..64012)
                     /gene="lepB"
                     /locus_tag="ACHKUI_000468"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6596735.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(64028..65827)
                     /gene="lepA"
                     /locus_tag="ACHKUI_000469"
     CDS             complement(64028..65827)
                     /gene="lepA"
                     /locus_tag="ACHKUI_000469"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6596736.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(66025..66504)
                     /gene="rseC"
                     /locus_tag="ACHKUI_000470"
     CDS             complement(66025..66504)
                     /gene="rseC"
                     /locus_tag="ACHKUI_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6596737.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAIFGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(66501..67457)
                     /gene="rseB"
                     /locus_tag="ACHKUI_000471"
     CDS             complement(66501..67457)
                     /gene="rseB"
                     /locus_tag="ACHKUI_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6596738.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(67457..68107)
                     /gene="rseA"
                     /locus_tag="ACHKUI_000472"
     CDS             complement(67457..68107)
                     /gene="rseA"
                     /locus_tag="ACHKUI_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6596739.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(68140..68715)
                     /gene="rpoE"
                     /locus_tag="ACHKUI_000473"
     CDS             complement(68140..68715)
                     /gene="rpoE"
                     /locus_tag="ACHKUI_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6596740.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(68712..68867)
                     /gene="rseD"
                     /locus_tag="ACHKUI_000474"
     CDS             complement(68712..68867)
                     /gene="rseD"
                     /locus_tag="ACHKUI_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6596741.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            69123..70745
                     /gene="nadB"
                     /locus_tag="ACHKUI_000475"
     CDS             69123..70745
                     /gene="nadB"
                     /locus_tag="ACHKUI_000475"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6596742.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(70730..71467)
                     /gene="trmN"
                     /locus_tag="ACHKUI_000476"
     CDS             complement(70730..71467)
                     /gene="trmN"
                     /locus_tag="ACHKUI_000476"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6596743.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            71599..72933
                     /gene="srmB"
                     /locus_tag="ACHKUI_000477"
     CDS             71599..72933
                     /gene="srmB"
                     /locus_tag="ACHKUI_000477"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6596744.1"
                     /translation="MTVTTFSELELDESLLESLQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(72966..73847)
                     /gene="yfiE"
                     /locus_tag="ACHKUI_000478"
     CDS             complement(72966..73847)
                     /gene="yfiE"
                     /locus_tag="ACHKUI_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6596745.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            73950..74537
                     /gene="eamB"
                     /locus_tag="ACHKUI_000479"
     CDS             73950..74537
                     /gene="eamB"
                     /locus_tag="ACHKUI_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6596746.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(74593..74976)
                     /gene="grcA"
                     /locus_tag="ACHKUI_000480"
     CDS             complement(74593..74976)
                     /gene="grcA"
                     /locus_tag="ACHKUI_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6596747.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            75281..75970
                     /gene="ung"
                     /locus_tag="ACHKUI_000481"
     CDS             75281..75970
                     /gene="ung"
                     /locus_tag="ACHKUI_000481"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6596748.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTALGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLLAESE"
     gene            complement(76018..77055)
                     /gene="yfiF"
                     /locus_tag="ACHKUI_000482"
     CDS             complement(76018..77055)
                     /gene="yfiF"
                     /locus_tag="ACHKUI_000482"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6596749.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            77262..77681
                     /gene="trxC"
                     /locus_tag="ACHKUI_000483"
     CDS             77262..77681
                     /gene="trxC"
                     /locus_tag="ACHKUI_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6596750.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            77750..78448
                     /gene="tapT"
                     /locus_tag="ACHKUI_000484"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             77750..78448
                     /gene="tapT"
                     /locus_tag="ACHKUI_000484"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6596751.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            78480..81140
                     /gene="pat"
                     /locus_tag="ACHKUI_000485"
     CDS             78480..81140
                     /gene="pat"
                     /locus_tag="ACHKUI_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6596752.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIARLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLSDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAKQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            81254..82609
                     /gene="pssA"
                     /locus_tag="ACHKUI_000486"
     CDS             81254..82609
                     /gene="pssA"
                     /locus_tag="ACHKUI_000486"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6596753.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            82655..82978
                     /gene="yfiM"
                     /locus_tag="ACHKUI_000487"
     CDS             82655..82978
                     /gene="yfiM"
                     /locus_tag="ACHKUI_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6596754.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(82975..84273)
                     /gene="kgtP"
                     /locus_tag="ACHKUI_000488"
                     /pseudo
     CDS             complement(82975..84273)
                     /gene="kgtP"
                     /locus_tag="ACHKUI_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
     gene            complement(84617..85583)
                     /locus_tag="ACHKUI_000489"
                     /pseudo
     CDS             complement(84617..85583)
                     /locus_tag="ACHKUI_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(85827..87174)
                     /locus_tag="ACHKUI_000490"
                     /pseudo
     CDS             complement(85827..87174)
                     /locus_tag="ACHKUI_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT        20749 a        23907 c        21657 g        20912 t
ORIGIN      
        1 ttccactaat aacaaattgt tttaccattc atgcattaaa tataaatgat aaaacaatac
       61 attcacatga tattaagcaa taataaattc aacgcgtttt ttagatatta aaccgtgccc
      121 atgttggcag aaataatcca cagcaccaca agcttttaac acttgtaacg cctgatgaca
      181 ctcagggcaa agtgcctgga cggtaaagtt tttgtcgcaa gttgcacagt gagcagtctc
      241 gccattgcgt tcgagagggg ttttgcaggt tggacagttg agtgccataa atactcctcg
      301 cgtgttattc ggcttccctc tccgtgtgga aagggaaacc agataagtga ttacttactt
      361 ttcttgatat gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta
      421 cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg cgatcccatt
      481 acgtccagcg atttgcggaa gtagttcatc aggtaacgct tgtaggaatc aggcaggtct
      541 ttcacctgat tgccgtgaat caccacaatc ggcgggttat aacccccggc gtgggcgtat
      601 ttcagcttca cacgacgacc gcgtaccagc ggtggttggt gatcttcaac agccatcgtc
      661 atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata cgcttcacgt
      721 actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa gtgcacacga
      781 gcaaaatcga taaagcccag acggaagtcc agcgtctctt tcacctgctc tttcacttcc
      841 tggctcaggc cgtcccactt gttcactaca atcacaagtg agcgcccact attgagaata
      901 aagcccagca aggagagatc ctgatcggaa ataccttcac gcgcatcaat caccaacatc
      961 accacgttgg catcttcaat ggcctgcaac gttttgatta ccgagaattt ctctaccgca
     1021 tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata ctcacgtccg
     1081 tcgcgttcca ttggaatgta gatactgtca cgcgtcgtgc caggcatgtc gtaaacaaca
     1141 acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt cgggcgaccc
     1201 acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg
     1261 ccgttctctt ccgcttcaaa ttgcgcccag tattcggcgt cttcgtcgac ttcttcttgc
     1321 ggtgcgagat cttccatcca cggcagcagc acgtgctcca gcagacttaa tacgccacga
     1381 ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa gcgcgtagaa atcaaccact
     1441 gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg tttttcacgg
     1501 gagcgcagat gtttagcaat cgcttcatcc gccggcatca ggcccgcgcg cgcatccacc
     1561 ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc catgcgggtt
     1621 tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tgcagataaa ctcacggcct
     1681 tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc cgcaaccagc
     1741 gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt agggcgcccg
     1801 acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat cagacaacgc
     1861 acgctatatt cgtgtcgttg ttaaaaacag gaaaacggcc cctgtccagg agccgttttc
     1921 aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc ttttgcctgg
     1981 atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact atcaactttt
     2041 tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcagataacc ttcgctgtca
     2101 cctaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg cagcagatcg
     2161 ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg gtcattttga
     2221 tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt
     2281 ttccacataa tctgaccgct gcgcagatca agcgccgtca ggttaccatt ataggccagc
     2341 gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag acggtcaatt
     2401 tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc
     2461 agcactgcgc tgacgcgacc attatcgcca cccacgacgg ccgcaccaaa agccgttgcc
     2521 ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt ccatttgaca
     2581 gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat taacaccaga
     2641 ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg ccatgccaca
     2701 gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cggtgccaat gtagacatgc
     2761 ccaccagaca cggtcacgcc gccagaaagt aatgcaggat ctttagagaa ccagccatct
     2821 ttctcggcca ggttgacaga ccagatttct ttgccgtcat ccgcattcag cgcttttact
     2881 aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg aagattggaa
     2941 tagaagttgc caatgccgct accaacggaa gtgctccacg cagtagtcgg cgtaaactgg
     3001 ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt aaacagcgaa
     3061 cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg caattgcatc
     3121 gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc tcagtgccgg
     3181 ggtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct tatcaccttt
     3241 gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt cacctttgat
     3301 agcatcaagg gtttttagcg cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag
     3361 acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc cctgttgtaa
     3421 ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga
     3481 agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg ctgggatgct
     3541 atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag aagcggagcg
     3601 tgcagaatca acctgatggc tattccagta gcgccagcca atcagtgcgc caacgcccaa
     3661 aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa ccgcttcaac
     3721 ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta acccagtaac
     3781 gtgcgcaaat gcgcggctac gctatcctgc gcaactgcag tttgctcacc agagcgcaaa
     3841 tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac agcaacgcgg
     3901 gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc
     3961 atcagtttca cgcccggtaa ttcatcacgc agacgctctg ctaatgccat agccgcagat
     4021 tgtgtgtcag cacctgaagc caccaggtat atatcgacaa caggatcggc tttaaattcc
     4081 ggattaacgg cctgaactaa caatacaaga cgttcgaggc ccatcgcaaa accgactgcc
     4141 ggtgttgcac gaccacccag ttgttccacc agaccgtcat aacgaccacc tgcacacacg
     4201 gtgccctggg aaccgaggct gttagtcacc cactcgaaaa cggtacggtt atagtaatcc
     4261 agaccgcgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc aagcagtttg
     4321 cacagaccgg caaagtgctc acgagattcc tcgtccagat aatcgcctaa tgccggagcg
     4381 tcgttgagaa gcacctgcac ttccggattt tttgaatcga gtacgcgcag cgggttagtg
     4441 tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag gaatgccacc
     4501 agcgcatcgc ggtaattcgc acgagcttcc agcgaaccga tagagttcag ctcaagagtt
     4561 acgtgctcgg aaatacccag cgcgcgccac cagcgggcag tgagcataat cagttcggcg
     4621 tcgatatcag gaccttgcag accgaaaact tcgcagccca actgatggaa ctgacgataa
     4681 cgccctttct gcggacgctc gtgacggaac ataggcccga tataccacag acgctgttcc
     4741 tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg
     4801 cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt ttcaaccacg
     4861 tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat cggcaagcgg
     4921 atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat gcgctgccag
     4981 atgaccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc
     5041 acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca atgtttcggg
     5101 agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt tattattttt
     5161 caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc gcacgaatgc
     5221 gcgcttccag ctgatcgatc atatcgttgt tgtccagacg gtctttgcgc acgccatctt
     5281 catagaggcc gcttttcttg ttgccgccag tgacgccgag tgtagaaacc agcgcctcac
     5341 cagggccatt caccacgcag ccgataatcg aaacgtccat cggagtgatg atatcttcca
     5401 ggcgttgctc cagcgcgtta accgtaccga taacatcaaa ttcctgacgc gaacaggtcg
     5461 ggcaggcgat gaagttgatc ccgcgcgaac ggatacgcag cgatttcaaa atatcgaaac
     5521 cgactttgat ctcttcgacc ggatcggccg ccagcgatac gcgcagcgtg tcgccgatgc
     5581 cttcagacag cagcagacct aaaccaatag cggattttac tgccccgcta cgcgcaccac
     5641 cggcttcggt gatccccaga tgcagcggct gatcgatttg ttttgccagc aaacgataag
     5701 actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga tcgaagttca
     5761 ggcgatcgag atgatcaaca tgacgcatgg cagattccag caacgcctgc ggcgtcggtt
     5821 cgccatactt ttcttgcaga tctttttcca acgatccggc attaacgcca atacggatcg
     5881 gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca ttaccgatat
     5941 tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc gcaatgcgat
     6001 agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg agtttgaacg
     6061 cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca acacgttcca
     6121 gcgccttgat ttgattgacc gttgcttcga cgtctgtcgt acgcgtattg gtcatggact
     6181 gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata cgtgttgatt
     6241 ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac ccgtctgtta
     6301 ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag ttctgataaa
     6361 acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg gcgcaccaat
     6421 tttcagtttg tacggtgcct ggccggttaa gttcaaatta ccgtctttac gctgcatacc
     6481 gctaaacagt tttttaccgg tagcatcggt gacttccagc cagcaatcgg cagtaaagtt
     6541 catcaccagc gcattcgggt caaccgccgg cgtggtcacg ccagcctgat cggttggcaa
     6601 cggcgcagcg ccatctggtg ttgttgttgc cgctggtgcc ggagtcgcag cggtatcaac
     6661 atttgcctgc gaaggtggaa caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc
     6721 agttacggca ggtgtttgcg tgttggttgc ggtggtatcc acaggtgatg tcggcgtggt
     6781 cgccatagcc tgatctgtgg ttgtcgacgt atctaacggt acactctggc tttgattatt
     6841 attcagttcc gcagaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg
     6901 gtcttgccac caccaggcac cgctcaggcc aataaccaca aacaacacca gccaggtgaa
     6961 ggtcatcagc cagccgtcac gttttttgcg gcgtttaccg agggaaaaac tctgcatcgg
     7021 cgcaactttt gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc
     7081 tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag
     7141 atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg
     7201 ctcggcaacg gcctgttgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc
     7261 ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt
     7321 ttaaatgaga attgagatgc cggtgaacca tcatgcccac ccacaccgcg aaacatccgt
     7381 taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt
     7441 cactaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac
     7501 cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaagat tacgccacgg
     7561 tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc
     7621 tgcatccgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca
     7681 caagcagcat cgatatcatc accacgagtt ttacgcacaa tagtggtgaa accgtagctc
     7741 atcagcactt ttgagaaacg gtcgatacgg ctgttcgaac tgcgtccata cggcgcgccc
     7801 gggaacgggt tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc
     7861 agttggtgcg cgtgttcagt gccgtcgtta acgtgatcaa gcatcacgta ttcaatagta
     7921 actcggccct gattggcgtt ggatttctcc agataacggc gcaccgccgc aaggaacgtt
     7981 tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg
     8041 tgcagggaga tagccagcgc aacgtcgatc atatcgccca gtttatccag cgccggaacc
     8101 acaccggaag tggaaagcgt gacgcgacgt tttgacaggc caaaaccgaa atcatcaagc
     8161 atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc catacccatc
     8221 atcactacgt tagtgattgg gcgctgaccg gtgacttttg ctgcgccgac aattttcgcc
     8281 gcacgccaca cctggccgat aatttccgat acccgcaggt tgcggttaaa gccctgctgg
     8341 gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgtg
     8401 gcacggtcgt cttccgggat atacaccgtt tcaacgcgct gatcgccaac ggcgatcgcc
     8461 catttaatgg tgccgtcaga tgaacgctgt tcttcaacca cttccggtgc acggatttcc
     8521 gccacctctt tcagtttgcc gcgcaacact ttattgatgt cggtcatctc atcaaagttg
     8581 tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct
     8641 aaatctttaa aaaactcccg catctgctga cggttgagat ccagcaggtt gatttttcca
     8701 tctttcgtgg tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc
     8761 gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc
     8821 tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc
     8881 atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccgaag aaataagcga
     8941 tttcgcgagc ggcagactcg acggaatcag aaccgtgggt accattttcg gtcaggctgt
     9001 cagcgtaatc agcacgcaga gtaccggcca gtgcgtttgc cggattggtc gcgcccagca
     9061 gatcgcggtg acgctgaaca gcattttcac cttccagcac ggaaacaacg atcgggccag
     9121 aagtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc
     9181 cacgagcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag
     9241 cttcaaagcg cgcaaagata ctaccaatca cgttttttgc taccgcgttc ggtttgatga
     9301 tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta
     9361 ccagcgtacg agatggcgcg gattataatg agcaacaggg ccgttgacta ttgatgaagg
     9421 taacattttt ttaaaataaa cccagtttaa gcaacaatca gggacaaaac agactattgc
     9481 atgacaaatt tcactgtcgc tatttgcccc acgtcatcca tcaccagcaa ttgataatcg
     9541 cctttatccg tcaaatgcag ggtcacgttg cgcccacgtt cagttaacgg ttcgccgttc
     9601 agaaaccacc agcgttcacc tgcccctcca cttgattgca acggcaaagt cgcttccgct
     9661 gcgcccggta aacgtttaat aatcgcgcca tcgcgcacac ctgttagctg cagcgggagt
     9721 tgtgcatcgt gaccgtatgg cgggcaaatt gtcgaggccg gtggcaagcg cacagcgcga
     9781 cgttctgatg cgggcagcca gggttccagc ggtagcggcc agacatttat catttcttgt
     9841 cgcgcctgag ggcaatcagc ggcaacacgt ttgccgtttt catccagcca gatggggaaa
     9901 cgaatgccat taatgccttc ctgctccggc agtaatagag ttggcggctg gctcccgtcc
     9961 agcagccagg ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa agactgtccg
    10021 cccggccagc agataacgcc acgacttaca gagtcaggac gtgggtcttc cggcaaattc
    10081 acactgcgcg acagtaagat attattaacc tgattcaaca gcggtacggc actggcaaag
    10141 ccaaactgac caacaacggg cgtgccgtcc ggtctgccag tccagatccc aatgacatag
    10201 cgagcgttaa ccccaatcgc ccaggcatca cgatagccat agctggtgcc cgttttccat
    10261 gccagtgggg cgacgcgcgg caaggcacca tccggcaagg gttgcgcttc atcagccata
    10321 atccggcgaa tgatccacgc agcccccgac gacattaaag gccgttcaag cagcggatcg
    10381 tcaggctgta agcgcaattt acctgccttg ccgtggcgag caaacgcggt atacgctgcc
    10441 gccatatctt ccagttttgc gccagcgccg ccgagaatga gcgaaagatt cggtgcagca
    10501 ccgttgggca aatataacgg caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt
    10561 ccataggctt ccagcacctg cacagcaggt aagttcagcg atcgcaccag cgcctcactc
    10621 atgctgatcg ggccatgaaa accgctgtca aagttgccag gtcggtaatc accggtgcgt
    10681 ctggggacgt cttgcaacag cgatgccggg tggatcaagc cttcatccag cgccagacca
    10741 taaacaaacg gtttgagcac cgatcctggc gatcggatcg cattgaccat atcaacatga
    10801 ccaaagcgtg aatcatcgtt gagatcaacc gatcccaccc agccgcgaac acgcatatcg
    10861 gtatgatcaa ccacgatcat cgccagtgag ctgcgcggtg gcaatcgtcc tttccagttt
    10921 tgcgccagtt cttccagacg tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg
    10981 cttttgcttt taccgagcat cattcgcgaa aacagcggtg ccagttgcgg catttgtcgg
    11041 ggtgccagcc agatgggttc ttcccttgac tcttttaccc gctcacggga ccacacacct
    11101 tgcacggcca tccgttcgag cactttatta cgcgcggctt cggcacgctc cggccaacga
    11161 tccgggcgaa gacggctggg cgcttgcggc aacaccgcca gcattgccgc ctcggaatag
    11221 cttaaattcg caggcgattt cccgaggtaa gcccaacttg ccgcaccaat cccctgcaac
    11281 gtaccgccaa acggggcacg gttaagatac aaggtcagaa tttcacgttt agacagatgc
    11341 cattccagtt gcaacgcgcg ccagagctgg cgaattttgc cgccaaacgt tttggggtga
    11401 ggatcaagca ggcgcgccac ctgcatggtg agcgtgctgc cgccggaaat gacccgaccc
    11461 gacgtgagat cttgccaggc tgcacgcgcc accgagaacg gattcacccc cggatgcttc
    11521 cagaaccagc gatcttcata attgatcagc gcttcaaggt aacgtggaga aacatcttcg
    11581 attgttactg gataacgcca gatgccgtca gcatcggcga aacgccagag tggcgtacca
    11641 tcctgcgcca ccaccacgcg tgcaggattc acttcttgca atggcagagg ccagagttta
    11701 tctgctgccc atgctgccag aatgatgata aaaggcgcgg ctgccagcat tatccagcag
    11761 ccgcgtttgg ttaacaggcg aggcatttac ggtctgacaa tcagcaaatc ttcagccgcg
    11821 ccggtcgccc gccattgggg aacatacatt gattccacca tcggttgcgg tacctgatac
    11881 gttcccggcg tcaccgcccg cgccagatac accagcgtta ccggttgata ttcatcaacg
    11941 gcaaccgccg ccacaaagcg atcgtcacgg aactctatgt gcttaatgct cgcctgctgc
    12001 atctggttca gcaagttctg cacttcacca ccactttgct ccaggctggc gctgccgttc
    12061 gccagattct ggttttccag ttccagcccc gcaggcaaca gatccacgac taacgcatcc
    12121 ggtacactgt tactggcttt aacctgcaac cacaccagca ccagatcgcc gctacgtaac
    12181 gagtccagcg atttgctctt accgtcagta cccagaatat gacgctcaat ttgcagcaca
    12241 ttgctcgccg gtaaaggtgc ggattgcgga taaccgctgg catccacacg cagccataac
    12301 ggctgatcgc cactgttggt cacctgcaag gtggcaagtt gatcgctatt cagattgctg
    12361 gtttgcgctt tctcgcctgt caacggctca gcagagaaag tggtttgcgc ctgccatttg
    12421 cccggtaaat cctgaagagt acgggcagcc aggaacaacg cgttactttc ctgcgtcgat
    12481 agccagcgtt caccgaacgc ctgctgcgaa agtgtgttta acagggagta ctgctcatcg
    12541 ggtagcagtt tgttctcttc cagcaatgag agcatcaacg cgctgtctcg cagtggacta
    12601 ccgtaatcac ccagccatat ccgctcatca ctattacgcg gcgttttcag cgccagcgca
    12661 atcgcttctt caccacgcgt cgcatcaccc atgattttca gcgcaacgcc aagttgcagc
    12721 agcggtaaac cagaagcggc atctgcacga tgctcccaga tttcacgcag cgcacccagt
    12781 ggtgcttttt gctgacgggc caacaccagc gcagcgtaag actgtacggc gaatttactg
    12841 gctttgagat tatcagcgta cgggatcgac atcatgcccg gatcttgtaa atagcgcagc
    12901 agacgctcat taccccggtt aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg
    12961 cggaccagga aatccatcac gtaagccgtc agccagtact cttcgtcacc gtttttatcc
    13021 cacaacgcaa agccgccgtt atcacgttgc atttgcagca aacgggaaat gccgatatcg
    13081 accgatgcac ggcgtttctc atcactgtcg cctttgatgc ccaacgcctg cagttgggct
    13141 gcattggtat aaagtgacgg gaacaggccg ctggcggttt gctcgagaca gccgtacgga
    13201 tacgctttta actctttgat ataacgtgcg atgttcagcg gtggtttgcc actcaacaac
    13261 aattgccctt ccagcgtaac aggcgagaag ttttgcaatc catcagccgg gagcgcccag
    13321 gtttcaccag gctgtaacgc cgtaccgtaa ttgaccgttt gtgccgggaa tgccggacgg
    13381 acgccgattt tccactgctt atgctgatcg gcaacggttt cccccggtaa cgctaacccg
    13441 ctaatggtgg cctgaatgtc tccatcgcca taacccggca atgcacgcac cgggataaac
    13501 agcgtagtac gcacccctgg cgctaattca acaggtgcgg gggaattact caccagttca
    13561 agcaagccac tggcggtcag agcaacatta agtttttgcg gtttatcggt aagattagtg
    13621 atatccagcg tcaggcgcga ggtatcgcca ctcgccataa agcgtggcat gttcagttca
    13681 gcaatcaccg gtgcagcaac aatcactttg ctttcgttgc ttccgaagtc atctgccgtc
    13741 caggcttgcg ccatgacgcg caattcaccg ttaaaatcgc caattggcag tgtaaccgag
    13801 ccttcgccct gttcattgag cgttaccggc agcgcctgct gcgcgacaat attgacgtga
    13861 ttgaccggcg gtttaccacc acgtttcagc tcatcaccat cgccaccaaa acgcagagct
    13921 gccagacgcc cctgaccttc aatgacctga ccgtaaatat cgtaaatgtc tgcgccatag
    13981 cgtttctgac caaagaacgc ctgccacgga tctggcgtga cgtaatcagt aatattcagc
    14041 acaccgctat caacggcaga caccagcaca ttcacctgtt ttggcatctc accattttta
    14101 ttgctggctt taattttcac ggttaatggc tgattggggc gcattttggt aggggtttcc
    14161 agcgccagat cgaggcgacg gttttcatca ccaagcggca gatgcaacac cccaaccgcg
    14221 cgttttggcg tcgcggagcg agatttatcg ccaggacgta ccaccagcgt acttaaatac
    14281 agatcatgac gattccaggt tttatcgacc ggaatcgtca gatccagccc ttgagccgga
    14341 acatcaatct cttgccacca cagcggccct tcactggact cgaccatcgc ataaccttta
    14401 cccgccgttg gcgcggcaat atgcaactta atggtatcgc cagggcgata gctggcttta
    14461 tccagtttca gcgtgacacg gtcgggtcgc actgcaccac ccccgtcgct gttatcctgc
    14521 cagctataac cagcccagaa acgcacacta ctgaccgctt cattcggcgc tttaacttcc
    14581 agacgataag cgccccactc taccgggaaa ctgaccttac cggtttcgtc cgctttcaga
    14641 tccagagttt gttcattttc gatcagatct ttttgatcaa actgtgactg ccagccttca
    14701 tattccgacc agttccagta gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc
    14761 gacacggctt ttttcacgcc ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg
    14821 ccttcatcaa caatgggctg tttcaccgtg ctgtcggtgc gataatcgta aaccgatttc
    14881 gaggcgaact gcggacggat ccccggcaat gcatcggcag gccagatagc ctgctcagca
    14941 cggcgcgtca ccgggcgacc gcccgattcc agcaaactac cctggaaaat aacctgtaat
    15001 ggggaatgcg tttccttcca ctggctttct gtagaaactt cgccgcgccc tttatcatcc
    15061 agcgtcaact gaacttcatc cagcgtgcgg gaaagatttt cggcagctat atcgccgaat
    15121 tcaaaaccag gtaaggctga tacagcttca cgcagtgggc gcaggaaaag ttgcccttgc
    15181 aaagtattac cattagcagg tgcgccatac aggtagtacc ccaccacgga gaatttcact
    15241 tcatctttcg gcgttagcgg ggttttctca ccggtcagat tcagcgccat acgctctggc
    15301 ataaaatctt cgacgtggaa atcccacatc cgatactggt tatcgcccgt gttagcgcga
    15361 atatgccaca taccggttgc cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca
    15421 ttctccggct gactaacgac gctcctgagt acctgcccgt cgggtttaat cacgtccagc
    15481 ttgacaggtt gatcgggcaa cgctttaccg tctgcatcac gcagcaaacc attgaggatg
    15541 accgtttcac ccgggcgata aagatcgcgc gggccaaaca tgaaaaactg tttgctatag
    15601 cccggcgcgc cagcaatgtt aaattccgct aagtccagcg ccgggagttt taaatcgagc
    15661 agcgttgtct gaccgttttt acgcgccagc agtaatgccg cgtttttatc attttccagc
    15721 tgcacatgcc cctgagcgtc actgcttgcc tgagtcagag tctgcccttt ctcatttaat
    15781 aaagagactt caattccttg ctgggccgcg ccgttttcca gactttgggt aaagatatcc
    15841 agacgattgt gataacggtg agctgaaacg ccaatatcac ttaacgtaaa cagcgtcgcg
    15901 ggattactgt aatcgtaacg tccagcctga ttcatcacag ccagatacac gcccgcctgc
    15961 tgaagcggtt tgatatcgcc cagcggcagc aataattttt cacgggtgtt acgcgcagga
    16021 ttgagatcaa accgtccggt gtagaccaga tccgccatct gcagcagttt atctgactgc
    16081 cagttcgcca gcgaattgcg gtattcccac tggctaatga aagcgggcag tgattctggt
    16141 ttcacacgga agaagttaac atcgacattg ttaacgttaa gcgccattac cggcagccct
    16201 tcaacgacct taccaggaag caacgaacca cgactggcaa aaccgacgct gggttggata
    16261 tcgcgggtag ttatagtttt ttcgtcatct ttactaaagg ttgcgttatt gagcgctttg
    16321 acctccttgc caatagtgac gatcaaatca cgtttcggtt cgaggtggcg taaacgcagc
    16381 tctttaagat tatgtgacag ctcccaggca ccatccactt tgccgctttt tttatcgacg
    16441 acatgaataa cgcgtgagaa atcctgatcc gggtcgagag ggatggagaa cgtcagcacc
    16501 agcgtggctg cgccatcgag ttgaacttca gagagatcca gcaatgtcag cgccttaccg
    16561 gcactctgtt gggccagttt ttgtcgctcc ggcgcggaaa gttttgctga actcacgttt
    16621 tctgaagagg ccgctttagt aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg
    16681 ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca tgcaggcggc tacgcgtaac
    16741 tttttcattt ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg
    16801 tgagaattta cgttatgtaa ttcagtatca cgcccataca atccgaattt tagtgagaat
    16861 tgtggctcag acagccaaat cagcctcttt tgcttgtcgc ttttcactaa acatccgaca
    16921 atttagccta accccggcaa aaatggagat gcctatgtcc acgacatggt ttgtaggagc
    16981 cgactggctc gccgaacata ttgatgaccc ggaaattcag attatcgatg cccgcatggc
    17041 gtcgcctgga caggaagatc gtaacgttgc tcaggagtat ctgaatggac atattcccgg
    17101 cgcagtgttt tttgatatcg aagcgctttc tgatcacact tctccgcttc cgcacatgct
    17161 gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc aggataagca
    17221 cctgattgtc tatgacgaag gtaatctttt ctcagcccca cgagcatggt ggatgctgcg
    17281 cacctttggt gtagagaaag tgtcgattct gggtggtgga cttgcaggct ggcagcgtga
    17341 tgatctgcta ttagaggaag gcgcagtaga gctgccggaa ggagagttta acgccgcgtt
    17401 taatcctgaa gccgtggtga aagtaaccga tgtattattg gcaagccatg aaaatacggc
    17461 gcaaattatt gatgcccgcc cggctacacg ttttaacgca gaagttgatg aacctcgccc
    17521 aggtttacgt cgcggacata ttcccggtgc attgaacgtt ccgtggacgg aactggtacg
    17581 tgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc gcggtgtcag
    17641 ctacgacaaa ccaattatcg tcagctgcgg ttctggtgta acagcagccg tggttttgtt
    17701 ggcacttgcg acgctggatg tgacaaacgt gaaactgtac gacggcgcat ggagtgaatg
    17761 gggcgcacgg gcagatttac cggttgagcc cgtaaaataa gctttttaca ggcaataaaa
    17821 aaccgccgaa tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg
    17881 tattaccgta gtaatgcaag cgcgtctcag cggacacaat actcgccagt aactctcttt
    17941 ttgtcaagta aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc
    18001 cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagggcaag
    18061 cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt
    18121 cgcatggacc gcacaatgaa gatacggtgc ttttgtatcg tacttattgt ttctggtgcg
    18181 ctgttaaccg aggtaaataa taaccggagt ctctccggca acaatttact ggtggttaac
    18241 aaccttcaga acagcaagta agcccgaatg ccgccctttg gggcggcatg ttttaaatta
    18301 tccggttctg tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcgcaa
    18361 tatgttcggt aataaagtgg ctaatacctt tttccccttt tttcacctga caaatatcga
    18421 tgggttcatc gccaggtaat gtatcggtcg ctacacttcc cgccgcctga ataatttctt
    18481 cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt
    18541 taattgaaca aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtaagtg
    18601 aatcaatcat ttgcgctggc ggctctgcga cctccgataa tatcagcgat tcaccgcctt
    18661 ccagtacttc ctgactgcta agcggattcc cttcttcacc aatcagcaaa ctgatttcac
    18721 gcggcataaa ttctttaccg gttggcagtt tggcattaag gaagagcgtt tcgccaaggg
    18781 tcatctcaaa caatgtgcga actggcatta cgacaaatgc ctgttcgcct tcaaccgcct
    18841 gttgaagtgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc
    18901 agtgctgtaa atcaagtgcg ctatcttcaa ccacagcctc gccctgtgcc gccgtaccag
    18961 gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg
    19021 ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccataa
    19081 ttcattcaac gttcggggcg taagtgcctg atgcgctacg cttatcaggc ctacaaggga
    19141 accgcaatat attgaatttg caatactttg taggccggat aaggcgttta cgccgcatcc
    19201 ggcatattag tttacgccgt taacagatta gcaatcgtgc gcacaccaag tcccgtagca
    19261 cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc
    19321 caaccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct
    19381 gccggatacg ccgcgcttcc ggtattgttc agttccgcaa agttagacgg cagctggctg
    19441 cggtggaact ccgccagcgg cagacgccag aacggttcat tctcctgcgc ggcactcgcc
    19501 agcaagcgcc ctgccagcgc atcatcaaaa ctgaacagcg cgtgataatc attacccagc
    19561 gcggttttcg ccgccccggt gagggtcgcc gcatcaatga tcagttccgg tttctgcgca
    19621 ctggcatcaa tcaggccatc ggccagcacc agacgccctt cggcatcagt gttcatcact
    19681 tcaacttttt taccgttgcg ataggtaata atatcgccca gtttgaacgc gttgccgctg
    19741 atcaggttat ccgcacaaca gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg
    19801 gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt catcgagtcc
    19861 ataaacgcgg tctgtttgat gctgtagccg ccggaatcaa aagtgatgcc tttaccaacc
    19921 aggcacgcgt acactggcgc ttctttatcg ccagttgggt tgtaatccag cgccagcaat
    19981 accggagaac gttctgaacc gcgcccgaca gtgtgcagcc ccatataacc ttgctcacgc
    20041 agatcttcgc ctttggtgat ccggtaagtc acacgatccc ctgcgacgtt gctgatcaga
    20101 tcaacagcac gctgtgccag ttgcgatggc cccagctctt ctgccggtgc gttgatggtg
    20161 tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctgacgctg ggcatcgtcc
    20221 agatccggcc actccacttt acgtatgcct ttcggggctt tgtaaccttg ccagaatgcc
    20281 cagcagcgat ccgcatccca accttcaccg cttaactgaa catgcttaat gcccagaccg
    20341 tcaaccttgc gcgccgcacg ctggatcaac cccagatcgt ctgtcccgtt caaatgcagg
    20401 gtaatgccat cattattaat gctgtaagtc gctttttctc cccaacgtgc gtcggcaggt
    20461 tggggagaga gggtaatctt catcgcttct gtcattttag ttatccttat tcgtttaaca
    20521 gcaaaacggg ctacccccag gcagcccgtt cattcactcg gcttcgtcca gccagaccaa
    20581 caaaatcgct tcgaggattt tctcgttcga tgcctgcggg tcgtcgtcga aatcttccag
    20641 agcgcaaatc cactgatgca tatcggtgaa tcgaaccgtt ttcggatcaa tatcaggata
    20701 cgcatcgtac agtgcttcgc caatttcgcg gctatcggtc cacttaagtc ccatactaac
    20761 ctctgttaat gctcacgcgc atggttgata gtgtaacgcg ggatttcaac cactaaatct
    20821 tcgtcggtga cgcgcgcctg gcagcttaaa cggctttccg gctccagtcc ccaggctttg
    20881 tccagcatgt cgtcttcctg ctctgagctt tccggcagcg agtcaaaacc ttcacgaacg
    20941 atgcagtggc aggtggtgca agcacaggat ttttcacagg cgtgttcaat ctcgataccg
    21001 ttacgcagcg caacgtcgag aatggtttca ccgctattcg cttccagaac agcgccatca
    21061 gggcagagat cctgatgagg caaaataaca atctttggca tattaaacct cgtccacgga
    21121 atggcctttc agcgcacgac gaaccgactg gtccatgcgg cgagcggcga aatcctgggt
    21181 ttgtttgtct acgtttttaa tcgcttgttc gatggcgtca acatcatcgc cctgcgccac
    21241 ttcactcagg tgagcagcag catcgtcgat agcctgacgt tctgcggcgc ttaacagcgc
    21301 ggcatcagca gccagcgcac cgtgcagact ttccagcaca cgcgccgctt ctactttttg
    21361 ttctgccagc attcgggctt ttacgtcctg ctcggcatag ctcattgagt ctttgatcat
    21421 cgaggcgatt tcgctatcgg tcaggccata agacggtttg acctgaatag acgcctcaac
    21481 gccggtggat ttctccatcg ccgtcacgct caaaagaccg tcggcatcga cctggaacgt
    21541 cacgcgaata tgcgcaccgc cagccggtag cgccggaata cctcgcagcg caaaacgcgc
    21601 cagcgagcgg cagtcctgca ccagttcgcg ctcaccctgc atcacatgga tagacatcgc
    21661 cgtctgacca tctttaaagg tggtgaaatc ctgggcgcgg gccaccggaa tcgtggtatt
    21721 acgcgggatc actttctcca ccaggccgcc catcgtttcg aggcccagcg acagcgggat
    21781 cacatccagc aacagcattt cgctgtccgg cttgttaccc accagaatat ccgcctgaat
    21841 cgccgcgcca atagcgacga ctttatccgg gtcgatagaa gtcagcggtg gacgaccgaa
    21901 aaattcgcct actcgttcac gcaccagcgg cacgcgagta gaaccgccca ccatcaccac
    21961 ttccagcact tcatcagctt ctacgcccgc atctttcagc gcgcgacgac aagccagtaa
    22021 ggttcgtttt accagtggcg cgatcagttc attgaattgt tcacggctga tttcgccctg
    22081 ccagcccgca acgttaacgg tcacggagtc cgcatcgctc agcgcgattt tggccgcaat
    22141 ggcagcatcg agcagttcac gctgaacgcg gttatcgcta cgatcaggaa tgcccgcctg
    22201 ctcgcgaatg taatccgcca gcagatggtc gaaatcatcg ccgccgagcg cggaatcacc
    22261 gccggtcgcc agcacttcaa acacgccgcg acttaagcgc agaatggaaa tatcaaacgt
    22321 cccgccgccg aggtcataaa cagcgatcac gccttcctgg ccggaatcca gcccgtaggc
    22381 aatagccgca gcggtcggtt cattaagtaa gcgcagaaca tgcagccccg ccagacgcgc
    22441 cgcgtctttg gtgccctgac gctgggcatc gtcaaagtac gctggaacgg tgataacgac
    22501 accatccagc tcgcctgcca gggcttcagt tgcccgcgcc gccagtgctt tgaggatgtc
    22561 cgcagaaacg cgcaccgggt tcagcagccc cgccgccgtt tcaatcatcg gcaggccatt
    22621 ttcgctggcc tggaattgat aaggcagatg cggatagcgt tgctggatat cagccagcga
    22681 gcgccccatc aggcgtttaa cagaactaat ggtgttggcg gtgtcgagcg ctgcgttagt
    22741 acgcgcgtca taacccaccg aatgcccttg ctgttgatag tgaacaacag atggcagcag
    22801 gtgacggcct tcatggtcag ctaacgtttc ggcctgaccg ctgcgcactg ttgccaccag
    22861 cgagttggtt gtgcccaggt caataccggc cgccaggcga cgctgatgcg gcgcagcact
    22921 caaaccaggt tcactaattt gtaataaggc catgtttagc ttccagaaat taaaaatcga
    22981 gcagtttttc ttcgagttgt tcggcactgc tccgcagttt atcgagaaaa cgcagcttac
    23041 gcacggtatc cgccgccgcg tcccacgtct cgttgtctaa ctgttcaacc atcaactgat
    23101 ggcgggtatc aaacatcttt ttcacacgtt tgataaagct ttccagccgc gcttcatctt
    23161 tcgcctgttc gatctcgtcc agttcttcgc gcagttccag ctgttccatc aggaacgcgg
    23221 tgtcgcgcac agtatgctgc tcgctggcga gatcaaaacc gtgcaaagaa agcaaatatt
    23281 ccgcgcgcat taacggatga cgcagcgttt gccaggcctg gttaatggtc gcggattgct
    23341 gtacggcggc gagttgttcc gcctggcttc cgctggcgaa tttatcagga tgatactgac
    23401 gttgtagatc ctgaaaacgc aggctcagcg cctgggtatc gagttgataa cgggcaggca
    23461 agccaaagag ggtgaagtaa tccataacat tctcagggct gcgatccgcc gcgataacgg
    23521 cgaatcaggt aaaacaaacc ccacgcgcag gcgaccacgg tggggttatc ggtatgcgca
    23581 tcaaacgtgg aagctttcgc cgcaaccaca ctcatctttg acgttcgggt tggtgaattt
    23641 aaacccttcg ttaaggcctt ctttgacgaa gtccagctgc gtaccgtcca gaaattgcag
    23701 gcttttgcca tcgaccacga ctttcacgcc tttgtcttca aacacgatgt cttccggcgt
    23761 cggttcgtca acaaattcca gtacataagc catacctgaa cacccggagg ttctcacgcc
    23821 cagacgcagg ccaaaccctt taccgcggtt agccagaaag gtatttactc gcgctgctgc
    23881 actgtcgctc agtgtaatcg acataaccaa acctcaactc ttattttgct tcacgtttgc
    23941 ttttatagtc cgcaatggcg gctttgatcg cgtcttctgc cagaatagaa cagtgaattt
    24001 tcaccggcgg cagttcaagt tcttcagcaa tatcggtgtt ttttatcgcc tgtgcttcgt
    24061 cgagagactt ccctttcacc cattcggtga ccagggagct ggaagcgata gcggaaccgc
    24121 agccgtaagt tttaaaacgc gcgtcttcaa tgataccttc atcgttgact ttaatctgca
    24181 acttcatcac gtcgccacag gccggtgcac ccaccatgcc gctgccgacg ttctcgtcgt
    24241 tgttgtcaaa ggaacccacg ttacgcggat tctcgtaatg gtcgataact ttttcgctgt
    24301 aagccattat aaattctcct gattccgata ccgattaatg atgagcccat tcgatgctgt
    24361 tcagatccac gccctgcttg tacatttccc acagcggaga aaggtcacgc agacgaccga
    24421 tggatttacg aactaactcg atggtgtagt cgatctcttc ttcagtagta aaacgaccta
    24481 aagagaaacg gatagagcta tgtgccagct cgtcgttcag ccccagcgcg cgcagcacgt
    24541 aggacggttc gaggcttgct gacgtacagg cggaacctga agaaaccgcg aggtctttca
    24601 gcgccataat cagcgactca ccttcaacgt agttgaagct gacgttgaga atgttcggcg
    24661 caccgtgttc caggtcaccg ttcaggtaaa cttcttcgat atctttgatg ccgttccaca
    24721 gacggttacg caggccgcgc agacgttcca tctcggtcgc catctcttct tttgcgatgc
    24781 gataggcttc gcccataccg acgatctggt gaacaggcag agtgccggaa cgcataccgc
    24841 gctcgtgacc gccgccatgc atttgtgctt cgatacgtac gcgcggttta cgacgcacat
    24901 acagcgcacc aatacctttc gggccataga ttttgtgacc ggagaaagac atcaggtcaa
    24961 ctttcaactg gctcagatcg atcggcagtt tgcccacgct ctgggttgca tcaacgtgat
    25021 agataatgcc acgagcacgg cacatttcgc cgatagccgc gatatcctgc accacgccga
    25081 tttcgttatt tacgtgcatg atggaaacaa ggatggtgtc atcacgcatc gctgcttcca
    25141 gttctttcag gtcgataatg ccgttacgct gcggtgccag gtaagtgact tcaaaacctt
    25201 cgcgctccag ctgacggcag gtatccagta ccgctttatg ttcggtttta ctggtgatga
    25261 tgtgcttgcc ttttttctga taaaagttgg ctgcaccttt gatcgccagg ttgtcagatt
    25321 cggttgcacc agaggtaaag acgatttcac gcggatcagc gccgaccaga tcggcaatct
    25381 gattacgggc gatatctacc gcttcttcag cctgccagcc gaaacggtga gaacgggagg
    25441 ccgggttacc aaaggttccg tccatcgtca taaactgcat cattttctcg gcaacacgcg
    25501 ggtccaccgg cgtggttgcg gagtagtcga gataaatcgg taatttcatt gctctataaa
    25561 ctccgtacat cactcaatgc aaggaaccag gctaccggct ggatgtacga ccgtgtttac
    25621 ggagtgttta gcactccggc ctgattctga attcttttta ttaagcgcgt aacttaacgt
    25681 cgatcgcgtc ttgtgtgcgg gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat
    25741 ccagcacttc ctggttatta accagttcgc ctaaagtaat gttattgaga aaaccggtaa
    25801 gacggtcgct caaatcacgc cacagcgcgt gggtcaggca tttatcgccg ccctggcagc
    25861 cgcctttacc ctgacaacgg gtggcatcta cagattcgtc aacggcgcta atcacttcgc
    25921 caacggcgat gctgctggca tctttgccta acagataacc accgcctggt ccacgtacgc
    25981 tggaaaccag accattttta cgcagacggg aaaacagttg ttccagataa gaaagggaaa
    26041 ttccctgacg ttcggaaata tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa
    26101 cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg tcttacttca
    26161 cctcaaactc gcccctgccc ggggtttttt attgtaaaat tgggggtatt gcatagcagg
    26221 gtcaagtctg acattcccga gtaaattggt caactattta cttgactgat ttagtcgggt
    26281 atttaacctt cagtgccatt tttttatcgt ggcgtttgta cctgttgtcg gatgcggtgt
    26341 gaacacctta tccgaccttg gattccgtgc acttacaggc ctgataagac gcgttaagca
    26401 tcgcatcagg caaccactgt tattcggcct tgttaccttt attctgctgc tcaatagaag
    26461 ccagaatccc gcgcaggata ttcaattcct ggctttccgg gcgtgcacgg gtaaacagac
    26521 ggcgcaattt attcatcacc tgccccggat ggttttcacg gataaaaccg gttgccagca
    26581 gcgtttgctc cagatgacca taaaaacgct ccagatcatc gaccagcgga tacggcgtct
    26641 cttcatgctc gacctgctcg ccattttcct gagttgccag ccaggccatg cgcacttcat
    26701 aggcaataac ctgaaccgcc atcgccaggt tcagcgaact gtattccggg ttagccgcaa
    26761 tcgcgacatg ataatggcat ttctgcaact cttcattggt caagccgacg cgctcgcgac
    26821 caaacaccag cgccaccggg gtatttgccg cttcagcgac gcttttcaga ccgcattcgc
    26881 gcgggtcgag catcggccac ggcagcgtgc gggagcgagc gctggtgccc accaccagac
    26941 tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg agcattacca atcacatcgc
    27001 tggcccctgc tgccagggca atcgcctggg agtctggttt cactagtgga ttaaccagcc
    27061 acagattggt taatcccatt gttttcatgg cacgggcaac agaacccata ttgccggtgt
    27121 gtgacgtctc caccagcaca attcgaatat tttgcagcat tgtctttctt catctaaaga
    27181 ttattcacgc atcttatcat aaaacgaaga cagatgccga tctcgctgct atactctgcg
    27241 ccgttttccc gttctttaac atccagtgag agagaccgat gcatccgatg ctgaacatcg
    27301 ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa aaactatgaa accccggacg
    27361 ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac caacgtagat aaagctgccg
    27421 aagcggtgat tatcgacacg attcgtaaat cttacccaca gcacaccatc atcaccgaag
    27481 aaagcggtga acttgaaggt actgatcagg atgttcaatg ggttatcgat ccactggatg
    27541 gcactaccaa ctttatcaaa cgtctgccgc acttcgcggt atctatcgcc gttcgtatca
    27601 aaggccgcac cgaagttgct gtggtatacg atcctatgcg taacgaactg ttcaccgcca
    27661 ctcgcggtca gggcgcacag ctgaacggct accgtctgcg cggcagcacc gctcgcgatc
    27721 tcgacggtac tattctggcg accggcttcc cgttcaaagc aaaacagtac gccactacct
    27781 acatcaacat cgtcggcaaa ctgttcaacg aatgtgcaga cttccgtcgt accggttctg
    27841 cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga cggtttcttt gaaatcggtc
    27901 tgcgcccgtg ggatttcgcg gcaggcgagc tgctggttcg tgaagcaggc ggcatcgtca
    27961 gcgacttcac cggtggtcat aactatatgc tgaccggtaa catcgttgct ggtaacccgc
    28021 gcgttgttaa agccatgctg gcgaacatgc gtgacgagtt aagcgacgct ctgaagcgtt
    28081 aatgactcag gcgggtgata tcactcaccc gccctcgcct ttcaggcgct attccgaaat
    28141 acttcctcac cgctttactt tctttcgtca ctctcccacc atctttcccc gattaatgga
    28201 tgataaagaa gtcgcaagga aagattatgg cgctgccagt gaacaaacgc gttctcaaaa
    28261 ttctgtttat tctctttgtt gttgccttct gcgtttattt agtgccgcgc gtcgccatca
    28321 acttcttcta ttatcctgac gataaaatat acggtcccga cccctggtcg gcggaatccg
    28381 tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg gtttattcct tcttcgacgg
    28441 gccctgctga caacgccatc gcaaccatca ttcatgctca cggcaatgcc ggaaatatgt
    28501 ccgcccactg gccgctggtc agttggttac ccgagcgtaa tttcaacgtt tttatgtttg
    28561 attatcgcgg gtttggtaaa tcaaaaggca cgccgtccca ggccggattg ctggacgata
    28621 cgaaaagtgc catcaatgtg gtgcgccatc gcagcgatgt aaacccacaa cggctggtgc
    28681 tgttcgggca gagtattggc ggggcgaata ttctggatgt tattggtcag gatgatcgtg
    28741 aaggcatacg tgcggtgatc ctcgactcca catttgcctc ttatgcgacc attgccaacc
    28801 agatgatccc cggcagtggc tacttacttg atgagagtta cagcggcgaa aattacatcg
    28861 ccagcgtcag cccgatcccg cttttactca ttcacggtaa agctgatcac gttatcccgt
    28921 ggcagcacag tgaaaagttg tatagcctgg caaaagaacc aaaacggttg atcctaatcc
    28981 cggatggcga acacattgat gctttttccg atcgtcacgg cgatgtttat cgcgaacaga
    29041 tggtggattt tattcttagc gcgttgaatc cgcagaacta acccatgatc gctagcacga
    29101 taatcattca caaaaccacc ttaagacatg ctaatccact ggtcagaaca gtttaaaatg
    29161 agaaaaattc tgtgatgctt gccaacattt ctgatgatta gcattccctt cgccatttcc
    29221 ctaagcaaac tttagctatt cttatcaatt atgcttatgg gagatctaca gatgatgcct
    29281 acgcttgctc caccatctgt cctttcggct ccccagcgcc gctgccagat cttgctgacg
    29341 ctctttcagc cggggttgac cgccaacacg gcaactttca gcgagcttaa tggtgtggat
    29401 gatgatattg ccagtcttga tatcagcgaa acaggacggg agatcctgcg ctatcatcaa
    29461 ctcacactga ctactggtta tgacggcagc taccgggttg aaggtacagt gcttaaccaa
    29521 cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc tgtgtccgtc atttattacc
    29581 agccgattca cccccgccct gaagagtgaa ctgaagcggc gcggaattgc gcgtaacttt
    29641 tacgacgata ccaatctaca agcgttagtg aatctctgct ctcgacggct gcaaaaacgt
    29701 tttgaaacgc gcgatattca tttcctgtgt ctgtatctgc aatattgttt gctgcaacac
    29761 cacgctggaa ttacgcctca gtttaatccg ctccaacgtc gctgggcgga atcctgcctt
    29821 gaatttcagg tagcgcagga aattggacgc cactggcagc gtcgggcgct ccagcctgta
    29881 ccgcctgatg agccactgtt tatggcatta cttttttcca tgttgcgggt tcccgatcca
    29941 ctgcgggatg cgcatcagcg ggacagacaa ctgcgtaagt ctatcaaacg tctggtaaac
    30001 cactttcgtg agctgggaaa tgttcgtttt tatgatgaac aggggttatg cgatcagctt
    30061 tatacccacc tcgcccaggc gttaaatcgc agtttgtttg ccatcggtat tgataacacc
    30121 ctgccggaag agttcgcccg cctgtatcca cgcctggtgc gcaccacccg cgcggcgctg
    30181 gctggatttg aaagtgaata cggcgtccat ctttctgatg aggaaagcgg tctggtcgcg
    30241 gtgatttttg gtgcctggct aatgcaggaa aacgacctgc atgagaaaca gattattcta
    30301 ttgaccggga atgatagcga gcgagaagcg cagattgagc agcagctacg cgaactaacg
    30361 ttactgccgc tcaacattaa acatatgtcg gtaaaggcat ttttgcagac aggcgctccg
    30421 cgcggcgcgg cactgattat tgcgccttat accatgccgt taccgctctt ttcaccaccg
    30481 ctgatctata cggacctgac gttgacaaca catcaacagg agcagatccg caaaatgctc
    30541 gaatcagcat gagggaacaa ctttcgggcg caaaaacatt gccggaagcg ccaccagcgc
    30601 catcacccag aacacgccgt ggccgagata ttgatacagg aaaccggcga aaacggtcat
    30661 gatggcgata ctgccgccca tcgcgacggc agagtaaacc gcctgtaaac ggatgacttc
    30721 gctaccctgg cgagcagcaa tatagcgcat ggcagccagg tggcagaccg tgaaggtacc
    30781 gcaatgcaga atttgcacca ctatcaacca cggcaacgcc gtagttgctc ccataatgcc
    30841 ccagcgcact acgccgcaaa tcgccgagat caacagcata tcgcgtgcac tacaacggcg
    30901 gaaaagttta ttactcagcg caaagataat gacttccgcc accacgccca gcgaccacaa
    30961 ataccccacc gccgaggccg agtagccagc tgcctgccag taaatggcgc taaaaccgta
    31021 ataggccgca tgtgccccct gcaataaaca aacgcaggcc agaaagcgcc agttctggcg
    31081 aaccaacgcc aaccacgcag accagccggt gctctcctgc tggcggcttg ccccttgtgg
    31141 ctgaatcgtc ggacggatga gaaagccgag cagcatggat gccacgccca acgtcaacag
    31201 cgcgaggatc acccgataat caaacatact gaccagtttg cccgtcagcg ccgagccaat
    31261 gacaaacgcc accgagcccc acagtctcac tttgccgtaa tcaagcggga actgcttttg
    31321 ccacgtattc gccagtgcat cggtcaacgg taccagcggt gagaaaaaga ggttaaagcc
    31381 aatcatcacc agcatcagcc acgctacgtg cgcccccgcc cagaaggcga cagcaaagag
    31441 aagtgtcagc agtgccagca cgcgcaaggc ggaaatcagg cgggaaggat cgctgacgcg
    31501 gggcgcgatg agcaaactcc cgaggaaacg ggcaaccaga cccgccccca ataacaggcc
    31561 gatggtttct ggcgttaaac caatcccttt aagccagacg ctccagaaag gtagaaaaat
    31621 gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac cagcgcgtgg attgcaaaac
    31681 catgagtccc tcccgtcagg aggcgttagt ctgaggttaa tcgaatgttg gtgcaagttg
    31741 aaaaataaga atattcgagc ctgcttcctg acggttatcc tgcatatcag gaaatgacaa
    31801 aaaagagcgg cccacaacac aacatgtaaa aaagaacgat gaaacaggtg aaaatcgcaa
    31861 tttattgaac tgacaaactc atgtgagacg gataaggcgt gcatgtcgca tccggcagga
    31921 aacaataagc acattgtcag ccatctgcgt tatgccgtta taccagaaac gaatatctca
    31981 acgcctgggc gtggcgaggt accagagtgt gtattacgtt taaatcacat catcttgcaa
    32041 agggattggt tatgaactca ctacgttatt ttgattttgg cacttcccgc cccgttttat
    32101 tattaattgc ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt cccaaaatga
    32161 tgggctttgg cggtacggtc caatatatgg cctcgctggg cgcaccaatg ccgatgctgg
    32221 cagcgattat tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg cttggctttt
    32281 tcacccgtcc gctggcggtg ctgtttattt tctacacgct gggtacagcg gtaattggtc
    32341 accattactg ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat ttctggaaga
    32401 atgtcagtat cgctggcgca ttcctgctgt tggcaattac cgggccgggg gcgatttctc
    32461 tcgatcgccg ttaggaaaga tgccggatgc ggcgtaaacg ccttatccgg cctaccgctc
    32521 caacaccgat tgtaggcctg ataagacgca gcaagcgtcg catcaggcat cgtgcaccga
    32581 gtgctacaaa atcgaaatta ccccgtaggc ctgtgcatcc ggcattttat aaattacagc
    32641 aacgcaaccc gcaacgacat ctctgaaatt gttgattccc ctggtgcaag cgcaatgagg
    32701 tcaccacctt ccggacgatg atggtcatcc ggcgcatggc tcatcggttc cagacagaag
    32761 aaatcaaacg catatccttt atcaaacgca gggtctgaaa caaagatgaa ataacacggt
    32821 gcaggtggcg tcgtttccat gatgatggca taaccctcct gcggctgctc gatgcgggcc
    32881 ttaccattcc atccggcaaa accattgttt acccactggc gcggcaacgg cgcgagctgg
    32941 ttaaaatcca gctcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc
    33001 tccagccagt aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa
    33061 ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agcgagcgtc
    33121 accgtcagcg tatccgccgt taaatgaaat gcctggctta cccgatagtg ataaacaccg
    33181 ctgagatgtt catacaccag acacaagcta tcatcgctgc gcgagacaca ttgccactcg
    33241 cccaaccagc catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga
    33301 tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggtaccagc
    33361 ggaaaacaag aggcatcggt tgccacaccg ctttttttac caggacgtag cagcggcgtc
    33421 gtatcgcgcc agaagccttc gattacgccg ccctgatcag aaacgtccag ctttagcggc
    33481 ccgtgagata aggtatagat ggtcatccgc agactcctta atccgggaaa ttaatcacaa
    33541 ctttcccgca tttgccgctc gccattagcg cgtaagcatc tcccgcctgc tccagtgaga
    33601 agcgatgggt aatagcgtta cgcggccaca gtttccagtc agtcaaatcg tgggcgcatt
    33661 tttccatatg gaacaagctg gtcacccagg agccaataat tctgcgttgg tgatgcatca
    33721 ggtcagcgct gacctcaaat tccacttttc cggtttcgcc aatgtaaacc actcgccccc
    33781 agtcggcggt agattgcaat gccagcaagc gccccgcagc attaccggaa caatcaagcg
    33841 caacatccgc accgccatga gtgagttcag cgatgatctg cggcagacct tcggtggtcg
    33901 ctaaatagcc gtgatccatc acccctagct gtttcgccat cgccagacgt tccggcagca
    33961 tatcaacgcc gatgatcctt tttgcaccac gacctttcgc cagcatcatc gccatcatgc
    34021 cgaccggccc cagaccgacc accagtacgt tatcactgcc ggaaacttcg ccgcgcaaaa
    34081 tcccttcata cgctgtacca acgccgcaac tgataaacgc accatcttcg taactcagtg
    34141 catccggcag gaggatcaga tctttttctt ccaccagcag gtattcagca tggccgccgt
    34201 cacgctgcca gccgtaagcc gcttttcctt cgccagtaca agaaatagga aaaccgcgac
    34261 ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt
    34321 taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga
    34381 taaatccctg gtataacggt ttatcgggtg ccgccgccgt agcgcggtgc tggtggtaga
    34441 tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa
    34501 tccccggcgt cggcaccgca acttcccgca gatcgacggt cgaatttcct ggtaaataag
    34561 ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc atccctgccg
    34621 cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgctacgct gggtcagcaa
    34681 gatattcgcc agcaccgcca ccacgatgat gacgccgcgc accacctgct ggaaaaagga
    34741 gttaatcccg agcagcacca gaccgttacc gattagcgta atcaccagta cgccaagcag
    34801 cgtaccgaac aacgagccgc gaccgccgga aagcgccgta ccgccgacaa cgaccgcggc
    34861 gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgcgccgc
    34921 caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaaaataa gaatgcgtac
    34981 ccgacgaacg ttaataccgc acaactgcgc tgcagtggca ttaccgccaa cagcaaaaac
    35041 cgagcgcccg aaggcggttt tgcggctaat gaacacaaac agcgcaaata acaccatcat
    35101 gatcagcgcg gataccggca ccccgagaaa ttgtccgccc agccagtcca gcacttcgtt
    35161 ttcgttaatt ggcactggca aggcgttcgt cataaacagc cccattccgc gcagggcgct
    35221 ccataaaccc agcgtggcaa caaaacttgg cacgttaaac acgccgcgca gcaccccggc
    35281 gagcgttccc atcagcgcac ctaacagcaa caccagcaga caagcaatcg ccagcggaac
    35341 gtcaaattgc agcaaaaacg ccaggcacac tgaaacaaaa gccaccatcg gcccaacgct
    35401 gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcga tcccaatggt
    35461 ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcattcag
    35521 ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg
    35581 actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga
    35641 catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    35701 atccacattg accggcgagt gaaactcctg cgagaacgta ccgtgctgta ataacaggat
    35761 gcggtcacac accagcggca actcctccac ttcactggag ataaacacca cgctttttcc
    35821 ttcggcagcc agctcgcgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    35881 acgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    35941 cactttttgc tgattgccac cagaaagcgt gccgatgggc gtttcgctac tggcggcctt
    36001 gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac
    36061 accgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca
    36121 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    36181 gtcgccgtaa tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata
    36241 cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcagaccagc
    36301 aatgccgagc acttcgccac gacgtagtgt aaaactgata tcctccagct tgggcttatg
    36361 gcgtaacgca cggacttcca gcacggcttg atccataatt tcctgagggg caaccggcgc
    36421 aatatcaacg tgatcgcgcc cgagcattag cgacacaata tgatgcgtgg aggtgttttc
    36481 gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg
    36541 gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt
    36601 tttcaccgcg ctgatcacca gttcaacctc cgcactggca agcgagctgg tgggttcatc
    36661 aagaatgacc acgcgcggct cgcccttcat cacccgcgca atttccacca gctgcttttg
    36721 cgccgggctt agtgttgaaa caagttgttc aggactaacg tcaacgccca acgcctgcaa
    36781 gcaacgttgg gcgtcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca
    36841 ctgaccgagg cagaggtttt ccgccactgt cagcccttcc accagactta actcctgata
    36901 aaccgcgcgt acccccagtt cagcggcacg gcgagtcagc gtagcttcgt caccttccag
    36961 tcgcgtctcg ccaatccaga tatcaccgct atccggacgt tcgctaccgg taagcattcg
    37021 aatgagggtc gattttcccg caccgttttt gcctaacagc gcacgaactt cgcctttatt
    37081 gagcgtgaag ttaacgttat ccagcgcaac gacgccggga taacgcttat ttcctgccac
    37141 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    37201 attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc gttttggtat
    37261 agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    37321 atgtttgcgt aaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca
    37381 gcacctggtt gttttccagc tcctgagcaa tttcagtggt catatcagaa ccgaaaacag
    37441 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgctccgc
    37501 ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccgacag
    37561 aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc
    37621 gggcttttaa tacttcttca aaccctttac gtcgttgcac acaaacttca aaggcttcgc
    37681 aattgatgac ggcaattttc ggctggtcaa ttttgttggc aataaaataa tcggcggctg
    37741 cgttacccag ttttttacca aattccagcg ggtcaccaac cagatatgcc gagacatatt
    37801 tatcgacacc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    37861 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat
    37921 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcgccctgag
    37981 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct
    38041 gacgcactcc ggcgtaatat ccctgggtat caaggtatat tgcgccaata gtcatttctt
    38101 tatcagaagc cctggcaaat agtgcgctac ccaatagtgt agccattaat aataaattac
    38161 gggttcttgt cattttttta ggcatagtgc ctcctgtagg gtttttattt acaacggctt
    38221 attttaatta tttgagcgtg agcggcagcg cgtgccgctc aaatattaca gttaataggg
    38281 tttagtgaac gtagctaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc
    38341 aggctggatt aatcgccatc aattcagcgc gagcagattc atagtgcgca acctccccta
    38401 atcccgcggc cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
    38461 cgctatacag cgacaacaag tcaggctgcg agacggcaaa gaaatccgct tcgatactgg
    38521 ttttcgccat ctctttcgcc cactgtttca tttcactaaa cagttgctgt gcggtgtgtt
    38581 gttcgcccag cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt
    38641 tgtaataact gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacacg
    38701 tcgcttcagt ttcatcgccc tgcgcgttgg cgcatactgc ctgccagaac cagatgtcgt
    38761 tgtcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat
    38821 gaagcagctc gctggcctgc tgcggctctc tgtcatcaag atgctgccag gcgcgtaata
    38881 actggttgag gataaactgg ctggtgacct tcccttcccc accttcccac gggtggaatt
    38941 tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgacc agtgagatgc cacaaattga
    39001 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg
    39061 ccagtcgttt ctccggtgtg acaccgctta acttatccag caaatcccgt tcgaaaagca
    39121 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
    39181 aatcgtgctg cttattccac gcatggatcg ccaacccgcg ccagccgtcg gcaaactccg
    39241 gcgacatctc tacgcaacgc tgccataagg caatggcttt accgtagcta cgcttgttgt
    39301 agtagaagca ggccagtaaa tgccgggcaa accagcactc ttcgatactc tccagcgccg
    39361 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaaggcatca atagctttta
    39421 cgaccagttc tccgcgttca gcttttggta gcaaactggc ttgcaggtaa agcggtagcg
    39481 tgcgctgaca gtccagtgcg ttcagcatat ctgccgccag ggcgggcatg ccccagttaa
    39541 tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacaaaga cctcgccact
    39601 gtacgagggc agattcgcta cgaccatcga accagttcag ccaccacaga gtagcgttca
    39661 gcggataatc acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
    39721 acaccagtag caggttatgc aggcaaagca cttcctgatt ggttgggcag gcgcgaagac
    39781 tttgttggca aaaatccaga ccagcgtcaa agttaccatt acgcgccgcc agccgtgcca
    39841 gaccataata gccgcctgct ttactgttgc cgctccagac cgcacgccag aaatcctctt
    39901 cggcttgttg atattgcccc tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc
    39961 cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag cgcctgagtg gcatacgcca
    40021 ccgcttgcgg gaaatcggca cggttatatt ccagcatcgc cagcgccagg ttacagcgat
    40081 aatccagcgg atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctcgcgt
    40141 gatgatattg ctccagatgt tgaccgataa accaggcttc atctgtactg gtaatgtctt
    40201 gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa cgcttgcgac tgatgttcat
    40261 gataactcag tacaatattg ccgtcggcat cggagagctc aatggtcagc ctttcaggat
    40321 tgataccgtg caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga
    40381 gcaacgcgtt gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga
    40441 tggcatacag cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc
    40501 gggaggcatt ttgcaccatg cccagagaat gataaggcag gaaatactgc tcgaaacgct
    40561 tctcctcgta agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca
    40621 tcagttcgat atacgggccg ttattgtcag tcaggctctt atcccacgcc tggccaaatt
    40681 cactgtgtcc ccagctccac tgttttttac ctggcgcaat atggtggttg gcaacgtgta
    40741 gcaaaccacc atcttcatcg tgacaccacg cgccaacaaa atcgtactgt gatttttcag
    40801 ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact ccggcggagt
    40861 agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttaccgt
    40921 gatcaaacac cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca
    40981 ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcgac
    41041 tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
    41101 cgtgcatcgg ctccgtttcg ccgacccaca ccgtctgtgc gccgtcgtca tgggcttcga
    41161 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
    41221 tcccgccgga gatccacggc cccagcagtc ccaccagcgc cggtttaatg acttcattgt
    41281 gataaacaaa atcgcgctgt ttcactttat cccacgcgcg atgcacccga ccgcccagtt
    41341 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca
    41401 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
    41461 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
    41521 tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
    41581 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
    41641 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
    41701 tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
    41761 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcgg tcagtaatat
    41821 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa gccagacgcg
    41881 cgggcatagt agcggtacat ggctgattgc gccggaagga gactggacgc tgtgcctgaa
    41941 cgtgacgccc accagcattg agtgtcaggt cgctaatgcc tgtttaagtc cgaaaggcga
    42001 atttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
    42061 atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg ctctggcaat
    42121 ccacggtcag gttgatccag tgaccggcgt gtcgcaaacc atgccgcaag cgccgtgggc
    42181 aacaccgatt gaggtgaagt acctgctgga agagaagctg ggcattcggg taatggtcga
    42241 taatgactgc gtgatgctgg cgctggcgga gaaatggcaa aataattcgc aggaacggga
    42301 tttctgcgta atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
    42361 ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg
    42421 cgtcgtctgc gactgcgggc gttatggctg cctggaaacc gtcgcctcgt taagcgcatt
    42481 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga
    42541 aaaattgact accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag
    42601 ttgggttgac cacagtgcca acgccattgg tttgagtctg tataacttcc tcaacatcct
    42661 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa
    42721 taccattatt cgccagacgg gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
    42781 agcgacacag attggctttg ggcaattaag ccgtgcacaa caggtactgg gaattggcta
    42841 tttgtatgtt gaggcgcagt tacggcagat ttgatggcgc gataacgtag aaaggcttcc
    42901 cgaaggaagc cttttttcaa cagattaacc gtttcgctta cgcgtaaacc gggtaacgtg
    42961 cgcagatgtc gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt
    43021 ccagcacgtc acacatccag ccagccagtt ctttcgcttc ggcttcttta aagccacgac
    43081 gggtaatcgc cggagtacct acacgaatac cggaagtcac aaacgggctc ttcggatcgt
    43141 tcggtacgct gtttttgttc acggtgatgt tagcacggcc cagagcggcg tctgcttctt
    43201 taccggtcag gtttttatca accagatcaa ccaggaacag gtggttatca gtaccaccag
    43261 aaaccacttt gtagccgcgt tcgaggaaca cttctaccat cgctttggcg tttttcgcga
    43321 cctgctgctg gtaagttttg aactcaggtt ccattgcttc tttcagagca accgctttac
    43381 cggcgattac gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt
    43441 ttttgtacag ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca
    43501 gggttttgtg agtggtggta gtaacaacgt gagcatgagg aaccgggtta gggtagacac
    43561 cagcggcaac caggcccgca acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt
    43621 cagcgatttc acgcattttc gcccagtcca ccacgccgga atatgcagag aagccaccga
    43681 taatcatttt cggtttgtgt tctttggctt gtttttccag atcggcgtag tcgatatgac
    43741 cggtagcatc gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag
    43801 aaccgtgagt caggtgaccg ccatgcgcca gattcatacc cagaacggta tcgcctggtt
    43861 ccagcaacgc ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag
    43921 cgtagtcagc gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa
    43981 catactcgca accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca
    44041 gctgagaccc ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt
    44101 cgatgtgctc ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat
    44161 aatcggcaat gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa
    44221 atttttggcc tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg
    44281 tgcgataaca ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg
    44341 ctttggagaa taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa
    44401 tttttcactt catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct
    44461 attttttata agatgcattt gagatacatc aattaagatg caaaaaaaag gaagaccata
    44521 tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg gtggaaacgg
    44581 gaccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca gaactcaaag
    44641 aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg tttaacgcta
    44701 ttgccgccta cgccagcaat attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa
    44761 tcgcgcagaa gcacaccagt ttccagatca aaccggaaca gtacaacatc gtcggtgaac
    44821 acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg gacgcgtggg
    44881 gtaaagccta tggcgtcctg gctaatgtat ttatcaatcg cgaggcggaa atctataacg
    44941 aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt gtggctaaaa
    45001 caccacgcag cgcacttatc accagcttcg aactggagcc ggtcgacggt ggcgcagtgg
    45061 cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt ttcccgcatc
    45121 aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat cgtattgcgg
    45181 tgaaacgcga agagggtggg caggtatcca actggctgca caatcacgcc aatgttggcg
    45241 atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca gatgacacac
    45301 cagtgacgtt aatctctgcc ggtgtcggtc aaacgccaat gctggcaatg ctcgacacgc
    45361 tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa aatggcgatg
    45421 ttcacgcctt tgccgatgaa gttaaggaac tgggtcaatc actgccacgc tttaccgcac
    45481 acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt gatagcgaag
    45541 gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg cagttctatc
    45601 tctgcggccc ggttggcttc atgcagtttg ccgcgaaaca gttagtggat ctgggcgtga
    45661 aacaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtga tttgatgttg
    45721 ccggatggac acatccggca acccttgacg cgttttaaat tgccgcgtcg tcctcttcac
    45781 cggtacggat gcgaatgacc cgtgccacgt cgaagacgaa gattttaccg tcaccgattt
    45841 tgccagtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg tcgtccggta
    45901 cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgca ccgcggtaca
    45961 gctcggtatg gcctttctgg cggccaaaac ctttcacctc agtgaccgtc atgccggtaa
    46021 taccgacttc agccagtgct tcgcggacat cgtccagctt gaagggttta ataatcgcat
    46081 caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc taaacgtaac
    46141 acataacgcc aattcattcc ttgaaatcgt ttgcatccag ttcgtgtcgg gaaagcagtt
    46201 tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg
    46261 tgatttgcag cagcttacgc aaatagttga gttcaaactg attacgcgcc tcaacaaagg
    46321 ttggcagcgc cgtgttttcg ccctccagcg cctgctccac cagcgcatca ctaattaccg
    46381 gagatgatgt cagcgccacg cactgttcaa tcacgttgac caactggcgc acgttgcccg
    46441 gccagctcgc agtcatcagg cgtttcattg catcggtgga gaacgcgcgg acaaacggtt
    46501 tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg tcttctgtgc
    46561 gctccgccag ggccggaatt ttcaggctga caacgttgag gcggtaatag aggtcttcac
    46621 ggaattcccc gcgcgtcatc gcttttggca ggtcgcggtg ggtcgcagaa ataatccgca
    46681 cgtcgatatc aatatcgcgg ttacagccca gcgggcgcac tttacgctcc tgcaacacgc
    46741 gcagcagttt gacctgcaac ggtgcgggca tatcgccaat ctcatcgaga aatagcgtac
    46801 cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta aacgcgccac
    46861 gtgcatgacc aaacagctcc gactccagca attgctcggg taatgcgcca cagttaatag
    46921 caataaatgg cttgctgttg cgcgggctgg cgttgtggat agcctgggcg aaaatctctt
    46981 tcccggtgcc gctctggccg ttaatcaaaa cgctaacgtc tgattgtgcc accagccgcg
    47041 cctgttccag caaacgcagc atcagcgggc tgcgggtgac aatcgcctcg cgccagcgtt
    47101 cgtcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat agcgcatctt
    47161 tatcgacagg cttggtgaga aaactgaaca cgccctgctg tgttgcagca acggcatcag
    47221 gaatagaacc atgcgcggta agaataatta ctggcattcc cggctgcact ttctggattt
    47281 cagcaaacag ctgcataccg tccatttcat ccatccgcag gtcgctgatg actaaatcta
    47341 ctttttcgcg attcagtacc cgcaatcctt cagcaccact ttccgccgtg accacactgt
    47401 agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg tcatcgacca
    47461 gtaataaatg cgcaggttta tggctcatca ggagtgacct catcggtgga tggcgcgggc
    47521 ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg ggtcgagagc
    47581 tggcgttcaa tatcggtcag gttttccagc ttgcgggtag tgagttccag ttgctgttgt
    47641 agaacgtgat gttgctggcg caaagtatcc agctcgctgt cgctggactg ctggagtttg
    47701 ctgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc gcgccaaagt
    47761 tgatacagtg ggcgaacctg tgccgggatc tcggtactta gcgcatcaat gcgcgcgacc
    47821 agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag
    47881 gtattttgcc agttgccgtc gtcatattgg cgggcttgct gacgcgactg cgcaggcatt
    47941 aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc ggttgattta
    48001 ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag ttgataaacc
    48061 ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgactctg cacgcaaccc
    48121 agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa
    48181 atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg ttgcaggcag
    48241 tttggacatg gacagcgcgg agaaaccaga gcgtacatgt cgtacgtgaa gatttcgagc
    48301 actgcccggg gccgaactga caaataaaat agcctgatgg gatgggctgt tagcattatt
    48361 tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga
    48421 ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt aatcccagac
    48481 cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt tcgaagatca
    48541 tggcgcgctc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata taaacccgcg
    48601 caccatgtaa actgctgcga aggcaaatgt taccggattc agccccgtag tgcaccgcat
    48661 tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg caagctgttg
    48721 ctttgagatc aacgtcggta tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa
    48781 ccactgtctc caccagcggt gctaactcaa cattctccag ttccaccgca ctgtccgcct
    48841 gtttacggtt gtaatcaagc agttgttcga tcagcttttg caaattgcgg ctgctgctat
    48901 caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca
    48961 gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga gataaatgtc
    49021 ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac caaagaatac
    49081 gttgcccaac cgagcgtaac tcgctcggtc cactgaacga gacgctattg cccagagaac
    49141 gcccttcccc cagccggttg atcatgcgct cgatattttt caccggaccg ataatcatcc
    49201 tcgtgaaaag cagtaccatc accagactca ccagaaatag caccagcgat tgccaaccaa
    49261 aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag aacaccactg
    49321 tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttccaga cgcgcggcgg
    49381 cagcagcatc gggaccgctg ttgttacact gaagttgagc cagattattc aaatcctgac
    49441 gtaatgcctg gtagagttta tcgtccggca gcacgcctgc gtgggcatcg agcatttcgc
    49501 tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc agcacgcagt
    49561 actgacgata gctgcgctcc atctccagtg ccgcgttggt catcgcttca ctgcgccggg
    49621 catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagtgca ttcaggcttt
    49681 gccaggcttg ccatgccagc accaacaggg gcagcagaat cagcaaaaat gccagcatta
    49741 ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggcgtta ctctcgtcag
    49801 acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc gggaattacc
    49861 cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg gttgatatag
    49921 ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc gaattttatg
    49981 tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta ctattatgca
    50041 gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgct gctttgaggg
    50101 gtttatggct tctccgctat ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa
    50161 cttaagataa acttattaga taatattaaa atcaaggagt taagtgtcac caaaaagcga
    50221 cacggcaaac cacccattgt cgtgatttaa agacacaaaa aagctcccga agttgggagc
    50281 tcataatagc ggttggtgct taatgccgga tgcggcgtga atgtctcatc cgggctgcaa
    50341 taccaatggg ttgacgactt accccaactg cttacgtgca ttgcggaaaa tgcgcatcca
    50401 tgggccatcc tcgccccagt tttccggatg ccaggagttg ctgacggtac ggaaaactcg
    50461 ttccgggtgc ggcatcataa tggtaactcg accactttca gtcgtgaccg ccgtaatacc
    50521 gttcggtgaa ccgttcgggt tagccgggta ggtttcagtg actttgccga agttatcgac
    50581 atagcgcagt gccaccagcc ctttgctttc cagtgccgcc agatgcgccc catcacgtac
    50641 ttccacgcgc ccttcaccat gagagacagc aatcggcatc tgcgagccca ccatcccctg
    50701 caacagcagc gacgggcttt gggttacttc taccagactg aaacgcgctt caaagcgatc
    50761 ggaggtgtta cgcacaaaac gtggccacaa ctcgctacct gggatcagtt cacgcagatt
    50821 agacatcatc tggcaaccgt tacatacccc cagcgccagc gtttgcggac gatggaagaa
    50881 ggttgcaaac tcatcgcgta cacggtcatt gaacaggata gacttcgccc aaccttcacc
    50941 agcgcccagc acatcaccgt aggagaaacc accgcacgcg accagggcgt ggaaaccctc
    51001 caggcccgtg cgtccagcca gcagatcgct catatgcacg tcgatagcat cgaagcccgc
    51061 acggtggaaa gctgccgcca tttcaacatg ggagttcaca ccctgctcac gcagtacggc
    51121 aactttcggg cgcgcgccag tggcaataaa cggtgctgcc acatcttcgt tgatatcaaa
    51181 cgacagtttc acattcaggc ccggatcggc gtcgtttgat ttcgcctggt gttcctgatc
    51241 ggcacactcc gggttgtcac gcaggcgctg catctgccag gtcgtttctg cccaccagac
    51301 acgcaacgtg gtgcggcttt cgctaaatac agtctgcccg ttggcggtaa tcacaaaacg
    51361 gtcaccggaa accgcctgcc caacataatg gacgcaatca gcaagcccat gctgtgccag
    51421 taccgcttca acggcttcac ggtcagcggc tcgcacctga atcaccgcac ccagttcttc
    51481 gctaaacaat gcagccagac gatcgtcacc cagagtggcg atatccgcgt caatgccgca
    51541 atgaccagca aaggccattt ccgccagcgt taccaccagc ccgccatcag agcggtcgtg
    51601 atacgcgagc agcttacgct gtgcaaccag cgcctgaatc gcgtcataga agcctttcag
    51661 ttgcgcgaca tcgcgtacat ctgccggttt gtcgccaagc tgacgataaa cctgcgccag
    51721 cgccgttgcg cccagcgcgt tattgccttt gcctaaatca atcagcagca gtgcgttatc
    51781 ttcggtagaa agctgcggcg tgatggtgtg acgtacatct tccacgcggg caaatgcaga
    51841 aatcaccagc gaaagcggcg aggtcatttc gcgctcttcg ctaccttcct gccagcgggt
    51901 tttcatcgac atagagtctt tacccaccgg tatcgtcagg cccagcgccg gacaaagttc
    51961 ttcacccaca gctttaacgg cttcatacag gccagcatct tcaccagggt ggcctgccgc
    52021 cgccatccag ttggcggaaa gtttaatgcg tttgatatcg ccaatttgtg ttgcggcgat
    52081 gttggttagc gcttcaccga ctgccagacg ggcagaagcg gcgaaatcca gcagcgcaac
    52141 tggcgcacgt tcgccaattg ccatcgcttc gccgtagtag ctgtcgaggc tggcagtagt
    52201 gaccgcacag ttagcgaccg gcacctgcca cggccccacc atctgatcgc gcgccaccat
    52261 gccggttacg ctgcggtcac cgatggtcac caggaaagtt ttctccgcca cggtcggcag
    52321 atgcagcacg cgtttcaccg cgtcagcgat ggtgatccct tcacgggcca gcgcgtcgcc
    52381 tttcgctttc agcgtttgta catcgcgggt catcttcggc gttttgccaa gcagtacgtc
    52441 cagcggcaga tcgatgggtt gattatcaaa atgacgatcg tgcagagaaa gatgtaattc
    52501 ttcggtggcc tcgccaatca ccgcgtaggg tgctcgttca cgcttacaca gttcgtcaaa
    52561 cagcggtaac tgatcggcag caaccgccag cacgtagcgt tcctgggatt cgttacacca
    52621 gatttccagc gggctcatgc ccggttcgtc gctcaggata tcgcgcagtt caaatttacc
    52681 gccgcgcccg ccgtcgctca ccagttccgg catggcgtta gaaagaccgc ctgcgccaac
    52741 gtcgtggata aacaggattg ggttggcatc accaagctgc cagcaacggt cgatcacttc
    52801 ctggcagcga cgctccatct ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc
    52861 atcagactga ccagatgcca tagaagacgc cgcgccgccg ccaagaccga tgttcattgc
    52921 cgggccgccg agaacgacca gcttcgcacc aacgttgatc tcgccttttt gtacgtgatc
    52981 ggcgcgaatg ttgccgatcc cacccgccag catgatcggt ttgtgataac cgcgcagctc
    53041 ttcgccattg tggctgttca ctttttcttc ataagtacgg aagtagccgt tcagtgccgg
    53101 gcgaccaaat tcgttgttaa acgccgcgcc gcccagcggg ccttcggtca tgatgtccag
    53161 cgcggtgaca atgcgctcag gcttaccgaa atcttcttcc cacggctgtt caaagcccgg
    53221 aatacgcaag ttggatacgg agaaaccaac cagaccggct ttcggctttg cgccacgccc
    53281 ggtggcacct tcatcgcgga tttcaccgcc ggaaccggtc gccgcgcccg gccacggaga
    53341 aatcgccgtc gggtggttgt gggtttcaac tttcatcaga atatgcgccg gttcctgatg
    53401 gaaatcgtag cggcccgttt cgtggtcagc aaagtagcga cccacttcag aaccttccat
    53461 gacggcggcg ttatctttat aagcagagag aacgtggtct ggcgtggttt cgaaggtgtt
    53521 tttgatcatc ttaaacagcg atttcggctg ctgttcacca tcgataaccc agtcggcgtt
    53581 aaaaattttg tggcggcagt gctcggagtt cgcctgcgcg aacatataca gctcgatgtc
    53641 gttcgggtta cgacccagct ttgtgaacgc atcctgcaga tagtcaattt catcttccgc
    53701 cagtgccagg ccaagacgca ggttagcgtt gattagcgcc tgacggccct gccccagcaa
    53761 atctacgctg gtgaccggag tcggttgatg gtgtgcaaac aactgctctg catcatctaa
    53821 agcaaaaaag accgtttcca tcatgcggtc atgcagttca gcggtcacct gctgccattg
    53881 ttcattggtc agcgtaccgg cttctatata gtaagccacg ccgcgctcaa ggcggtttac
    53941 ctgttgtaga ccgcaattat gggcgatatc ggtcgctttc gaagaccagg gagagatggt
    54001 gccaggacgc ggggtcacca gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg
    54061 gccatatttc agcaggcgtt caagttgtgc gtgctcatca tcgtttaacg gcgcattgag
    54121 gtcagcaaaa tggacatact cggcgtaaat attgtgaacc gggagcctgg cagcctgaaa
    54181 acgtgccagc agtttgttga ttcggaatgc cgacaatgca ggcgaaccac gcagaatttc
    54241 catcataagt ctctcgtctt ctaagctttc ggtgtaccca agggggggaa acgggcgtca
    54301 ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga
    54361 attagcaacg cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa
    54421 aatgccgctc atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc
    54481 gcacgacaca gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca
    54541 ttctggcact gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg
    54601 acaaccgtat cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcataccc
    54661 ccctgactta taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac
    54721 agtttgccga ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc
    54781 tgtttgacga cctcgataat ggtaacgccg acctgctggc ggcaggcctg gtctataaca
    54841 gtgagcgggt aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg
    54901 tttataaagt cggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca
    54961 ccgttgcacc gggtcacgtg gtggttaacg atctccagac cctgaaagac acaaaattcc
    55021 cggaattaag ctggaaggta gacgacaaaa aaggctctgt ggaattaatg gaagatgtca
    55081 tcgaaggaaa actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg
    55141 ttcacccgga actcgccgta gcgctcgata tcaccgatga acaaccggtg acctggttta
    55201 gcccgttaga tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga
    55261 atgaagacgg tacactggca cgcattgaag agaaatacct ggggcatggc gatgattttg
    55321 attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc
    55381 ccctgtttga gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc
    55441 aggaatcgca ctgggatgct caggccactt caccgacggg tgtgcgcggc atgatgatgt
    55501 taaccaaaaa caccgcgcaa agcctcggaa ttacggatcg taccgatgcc gaacagagca
    55561 tcagcggcgg cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg
    55621 agaacgaacg gatctggttt gccctcgccg cgtacaatat gggctatgcg catatgttgg
    55681 atgcccgcgc cctgacggca aaaaccaaag gtaatcctga cagttgggct gacgtaaaac
    55741 agcgtctgcc tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc
    55801 gtgggcatga agcctacgct tatgtcgaaa atattcgtaa atatcagatt agcctggtgg
    55861 gttatctgca ggagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt
    55921 atccggcggt atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt
    55981 cccagtcgtc atcaaactat ttgacccact ctccctctct gctgttttcc agaaaaggga
    56041 gtgaagagaa acaaaattaa tctgtcgagg attgcgcttt tttctgcgct ttaatttcct
    56101 ggcggcgcat gcgaaagaag tcactgagca acgccgcaca ctcatccgcc agtattcctt
    56161 ccgtaatttc cacccggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg
    56221 cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca
    56281 tcgctccggc acacattaca cagggttcaa gcgtgacata caacgtggcg tcgatcagtc
    56341 gataattttg catcaccaga ccgccctgcc gcagagccat gatttctgca tgtgcggtgg
    56401 gatcatgtcg accaatcgga cggttccagc cttcgccgat tacccgattg ttatgcacta
    56461 ataccgcgcc gaccggtact tcccgctcat cccaggcacg cttcgccagc gtcattgcat
    56521 gacgcatcca gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa
    56581 aagcgcggcg cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac
    56641 cgcgcggggt tacccgccag cgatgctggc aaaagtaaag cagcgggttg tcctggttac
    56701 tgtcgctata gccactatac agccgcagcg gcgtgccgat tttgcgctcc agttgcgcga
    56761 ccttttcatg tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaatct
    56821 gactggcgat aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca
    56881 gcggctgcgg agagccggta atcaaccata tatcagcatc ggaacttaac aggtaggtgg
    56941 ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc acgaaaccag cgtacgaaat
    57001 cggcctgcaa cgcctgcaaa cgcgcttcat tgtgaccaaa agtgcatccc cacagaagca
    57061 gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct atgatcggca
    57121 acaacggcag aacaagtaac gcattcagcg gttggcgacg cagtaaataa cgtaaaaaac
    57181 taccgaacat atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct
    57241 cgtgagttgc caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac
    57301 atagcctaac agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg
    57361 gaatttttaa ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc
    57421 atattgcctg ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta
    57481 tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc
    57541 tacaacctga tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca
    57601 gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcactta
    57661 agttagcgtt gagtgaagcg ctggcaagcc agccggaatc tccctccgtg cccattcata
    57721 accaaatccg cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata
    57781 ctgccgctat gtacgcaacg ttaaacgtta atagtgaaga gaaactgcat gaatgcgtgg
    57841 caatgttgcg ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg
    57901 cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata
    57961 tgcatgcgct actcgctaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt
    58021 cctacaccgg tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg
    58081 gaaaagtgct ggcgattacc ggctttactc ccaatgccct gcaacagcgt gcttctcatt
    58141 gcctgtatac cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg
    58201 cgcagggaat gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg
    58261 caccagaacg tattcgtcat agcgaagcgc tggtgaaaaa actggtctga gtaaagaatg
    58321 cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc
    58381 actaaaaaat gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttca
    58441 atgggcgatc atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac
    58501 gagacaccca cctgccagaa ggtgtgcccg atccccaata ctattgtgaa agatccgcaa
    58561 catgtcgaga cggaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa
    58621 atttaatatt ttgctctgga agtagagtgt taattatctt atagagaaac cttaacgcta
    58681 ttatatttat ttgattgatg acattagcat aattattcac taagttaatt tatatagtat
    58741 ctgcccagac acttatttat agttattaaa ggcgcgtccg attggttcac cggatgcacc
    58801 ttaagtacgt ttccttatgt tataagaaca gaaggatcag ctgtaaaaca gcaatgatga
    58861 ttttgatgac ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc ttaaacaagg
    58921 aatgcagccg ttcagattag cccttacatc tccccaaaac tgaacgtgcg agttattgga
    58981 atcgagtgct gcaatccaca ccagagcttt gacgacacca ctcgtttcaa tgggggaatt
    59041 cagtggcatg gtgtaaagca cagcaaaatt gccaataacg aaaccgattt taatgtactt
    59101 atcaaaaagc ggtcacgtta atcttttcat caatgtcgat ttcttcgtaa aggctcgcgt
    59161 tttttatgtc agagattgca agttgcttgt acaggtcaac gaaattgatc cataatcatt
    59221 ttcgttggaa tcgtgtgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac
    59281 cagttcgggg gttttacttt ataaagagaa cggtattatt ttaactttca ataattaccg
    59341 tggcacaagc atagtggcgc tcatcggcaa gcgttacatg catatttaca acgccgagct
    59401 tttccgccag ttttaatgcc tcaccccata gccgtagccg tggtttgccg agctcatcat
    59461 tgaatacttc aaactgatta aacgccagac cattgcggat ccctgtgcca aatgcttttg
    59521 ccgccgcttc tttcacagca aaacgcttcg ccagaaaacg cactggctgg tggtgcgttt
    59581 tccagatttc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg
    59641 cgatcaccgc ttctatgcga gcgatctcta caatatccgt acctaaacct aatattgcca
    59701 ttagccacgc gcttccagca tcagacgttt catttctgcc accgcatctt tcagtccggt
    59761 catcactgca cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc
    59821 ggcaatggct ttcacgttgt gataggtcag accgtgtccg gcgttaactt tcagaccgag
    59881 actagtggca aaggtcgcgg ctttggcgat acgcgccaac tcttgcgcct gttcggcgtc
    59941 agttttggca tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc
    60001 cgcagctttg atctgctctt catcggcgtc gataaacaga gaaacctgga tcccggcatc
    60061 tgccagacgt ttgcaggcat cgcgcatttt ttcacgctgc cctgcgacgt ccaggccgcc
    60121 ttcggttgtt acttcctgac gcttttccgg taccagacag caaaaatgtg gcttcgtctc
    60181 aacggcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag
    60241 cgtctggcgc aggatgcaca catcgcggtc ggtaatgtgg cggcggtctt cacgtaaatg
    60301 cacggtaatg ccgtccgctc ccgcctgctc agcaataaac gccgcctgca ccggatccgg
    60361 gtaagcagta ccgcgcgcgt tacgcagcgt tgcaatatgg tcaatgttga cgcctaacag
    60421 taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata
    60481 aactgccgga acagttccct gctctttaat ggtttaccgc caagatacgg cttaagcgcc
    60541 atgcgggtaa agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc ccgcgcgttt
    60601 aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac
    60661 cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca
    60721 caatgggtaa aattgacgcc atagcccaga tgcccgagca gtgccagttc aaagcggcgc
    60781 aacgcgggtt ctggcgtacc agtgaccccg gcaagagact gaatgcagtg caagtaatcg
    60841 aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg
    60901 tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca
    60961 ctgcgcagcg ttttgacttc gccacgcccg ccaaaacgta gcaagagagg ggtgaaaggc
    61021 tgtaatgccc ctttcagagt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg
    61081 cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta
    61141 tgcaggacaa atgcgcgctg ccagccttcc atcggagtta ctcttaaaga tcgtcaacat
    61201 aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca
    61261 gctcaaggtg aacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga
    61321 tggttttgat cttggcccct ttgttgccaa tgaccatctt cttctgccct tcacgctcaa
    61381 cgagaatcaa accgttgatg tcataaccgc cgcgttcgtt agagacgaaa cgctcgatct
    61441 ccacggtcac ggagtacggc agttcagcgc cgagaaaacg catcagtttt tcgcggataa
    61501 tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatggg
    61561 tcgcttcagg cagatgctta cgcacgattg ccgcgatagt gtcgacattc agcccggttt
    61621 cggcagagat cggcacgata tcgaggaagt tcatctggct tgccaggaac tgtaagtgtg
    61681 gcagcagatc ggctttttcc tgcacgttgt ccactttgtt caccgcgagg attaccggcg
    61741 ctttgccatc gcgcagtttg ttgagcacca tttcatcgtc cggcgtccag cgggtgcctt
    61801 ctacgacaaa aatcaccagc tcaacgtcgc cgatagagct gctcgccgct ttgttcatca
    61861 ggcggttaat ggcgcgtttt tcttccatat gcaggcctgg ggtatcaacg tagatcgcct
    61921 gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtttgc gccttgcggg
    61981 aggtgatgga gatcttctgc cccagcagtt tgttcaataa tgtggatttg ccaacgttcg
    62041 gacgtccgac gatagcaata aatccgcagt aacttttatc gatgctcatt ccagctccag
    62101 ttttttcagc gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc
    62161 aaccaccggt tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc
    62221 ttcgccacgt acctggacga ccagataagt cggcagcggc agatggcgac cctgcaaata
    62281 ttcttgcaag cgcgttttcg gatctttttg tttgtcacct gggctaattt cgtccagacg
    62341 ggtttgatac cagttaagga ttaatttctc gacggtttga atatcactgt cgaggaatac
    62401 gccaccaatt aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct
    62461 tttaagttca cctggcccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc
    62521 tgccagcgta ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac
    62581 acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa
    62641 aaattctaaa cgctcgttat gtttgctgct ggcactacga tgggttaatg cctgctgcaa
    62701 cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg
    62761 gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata
    62821 tacatggctc ggctaactgc ttcactgcag tttcagttta ccggtatatg gggaccaacg
    62881 ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta
    62941 tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt
    63001 gtcgccataa cggcgacaac gtgaacgaag atggctatta atggataccg ccaatgcgac
    63061 ttaagcgcac accagtcggc cattcgcctt cttgcttatc gaagctcatc cagatagccg
    63121 ttgcccgacc gaccagattc gcttcaggca caaagcccca gtaacggctg tccgcgctgt
    63181 tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag gttgccagtt
    63241 gttgccctgg ctgctggtaa tacatcccca cctggtcctg cgcaatcggc actgtcagaa
    63301 tgcggtgcgt cacatcaccc aatgtctctt tacgctcgga aagacgaatt ccattttctt
    63361 tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca ttacggcgtg
    63421 agaaggtctg aacgaaatcg ctcggttcca cgttggagta agtgaccggt agcgcgtttt
    63481 cgcacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag accggatcat
    63541 aagtgacttt atcgcccggc aaacccaccg cgcgcttgat gtaatcaagc tttggatctt
    63601 ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg atcagcgttt
    63661 tctggtagat aggatcttta atgccataag caaacttctc taccagaata aaatcaccga
    63721 ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa ataaacgaac
    63781 gcacaatcaa tacgatagcc agtaccggaa aaacagaagc accggtttcc agccagccag
    63841 gcttcggcgc aacctttttc agcgttgctt tatccagtga gtcaccggca gccgcctgcg
    63901 ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg caccataaaa
    63961 tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc atgccaactc
    64021 ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct tcctgcggca
    64081 gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc tgcagcagct
    64141 ttttcttacg actgatatcg ccgccataac atttagccag tacgttttta cgcagctgtt
    64201 tcacggtgga gcgcgcaatg atgtgcgtac caatcgctgc ctgaatggcg atatcaaact
    64261 gctggcgcgg gatcagatct ttcatcttct ccaccagctc gcgaccgcgg ttttgcgaat
    64321 taccacggtg ggtgatcagc gccaacgcat caacacgttc accgttgatt aatacgtcta
    64381 cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac gcataaccac
    64441 gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc gggatctcgt
    64501 acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg cgtttttcta
    64561 cgcacagcgt aataacgttg ccgagatatg cctgcggtag cagcatgtga cactctgcaa
    64621 tcggctcgcg cagttcgtaa atgttattta ccgctggcag cttggatggg ctgtcgacgt
    64681 agataacttc tcttgacgtg gtttcaactt catacactac agtcggcgca gtggtgatca
    64741 gatccagatc gtattcacgt tccagacgtt cctggatgat ctccatgtgc agcaggccaa
    64801 ggaagccgca gcggaaacca aagcccaacg cgctggagct ttccggctca tagaacagtg
    64861 aggcatcgtt caggctgagt ttacccagcg cgtcacggaa ggcttcatag tcgtcggaac
    64921 ttaccgggaa cagaccagca tatacctgag gtttgacttt cttaaagcca ggcagcgcct
    64981 tttctgccgg attacgcgcc agtgttaagg tatcgcctac tggagcgccg tggatatctt
    65041 taatcgcaca taccagccag cctacttcgc cacatttcag ttcagtacgg tcaacctgct
    65101 tcggcgtgaa gatgcccaga cgatcggcgt tataggtctg cccggtgctc atgactttca
    65161 ctttgtcgcc cttacgcaag gtgccgtttt taatacggat aagtgaaaca acgcccagat
    65221 agttgtcgaa ccaggagtcg ataatgagtg cctgcaacgg gccttccgga tcgccttccg
    65281 gcggcggaat gtcgcgcaca agacgttcga gaacgtcctg cacgccaacg ccggttttcg
    65341 ctgaacagcg caccgcgtcg gtggcgtcga taccgacgat atcttcaatt tcttccgcca
    65401 cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag taccggcacc acttcgagat
    65461 ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttctacgccc tgcccggcat
    65521 cgaccaccag cagtgcgcct tcacaggcag ccagcgaacg ggaaacctca taggagaagt
    65581 ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccatca gacgctttgt
    65641 agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca agatccatgg
    65701 aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc tggataatac
    65761 ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag ttacgtatat
    65821 tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt tagaagtcgc
    65881 tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac aacattgagg
    65941 cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc atttgttatg
    66001 caaaatgcaa caaagccagt gaaatcactg gctcgcgtct tccgaagatg tttcaaatcg
    66061 cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattcag accgggcagc
    66121 aaactttcga gagcagccac gtgcaatcag gaatccacca acgccaccga aaatcgcacc
    66181 gcataatgct gcgacgtcag aagcaaagag tagctgaaat agtgaagcga tgaggaatag
    66241 tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc cttcggcgat
    66301 tcctaattcc actttttgcc ccggcactaa cggctcatca cagggtacga caatggtatg
    66361 cgtggtttgc gggccaagtt tattcaacac gcggctaccg caaccggcgc gtgaagcgca
    66421 gctgctgcat gaggctttaa catcgcaact gaccagcgcc tgcccgtttt cccaggagac
    66481 gacggtagcc cactctttga tcattgcgct gccccgaact taatattctc ggcaatgcgt
    66541 ttcgccgttt gcggcggcag ttcaccgaca atggtgattt cggcgttatc acgtacgctt
    66601 gtactgacgg ttctgcgccc ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg
    66661 ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc gataggcatg
    66721 ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc ctgtggcagc
    66781 caggttggcg tccagctgaa tttagctttt tcaccgacag gaacagaaag caacggcggc
    66841 aaatttgcct tcgccagcgt ctgcatactg ctgccgatat cctgattgac gttaaaagca
    66901 atcacacgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac ccgcatcggt
    66961 aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc
    67021 acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga gataaagtcg
    67081 tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga atcaacaatg
    67141 taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat ttcattgccg
    67201 cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag aggacggtta
    67261 tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat gctgatgaat
    67321 gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa cgccccggac
    67381 gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa tgacatggca
    67441 aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta
    67501 cagcggcttg ctgagtttgt gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt
    67561 gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt acctgctgct
    67621 gttgaccatt atttgcggtc gcttcagaag gtactcccag actaaccggg ctggctttac
    67681 ccatcatcgg cagtgtatta aatactggcg tttcgggctg ctgggacgtt tcagattgcc
    67741 cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct acgcccattt
    67801 gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc tgccattgat
    67861 gcggcgcagg ctgggcttcc gggatcaatg tcgccgattg acgtactggc tcttcttcaa
    67921 tggcggccat cacgcgtgaa gagatatcga aatggagcac ctcgggagta tcaccccgca
    67981 ttgagtcacg gatcaagtga tagctttccc aggttttctg catttctggg ttatgagcca
    68041 gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa agttgttctt
    68101 tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga taagcggttg
    68161 aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg tacctaccgg
    68221 acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc gcaaggttat
    68281 tgccatgcgt aaatcttccg ggagggactc aatagttcgg aaaactatct gtctcagttc
    68341 ttctgacaac attaagttct cagggttcga aatttctttc aacgcgccgc cactttcgaa
    68401 gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct gagcaaccag
    68461 gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag cgctatctcc
    68521 ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca catcgggaac
    68581 atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat agcgcactac
    68641 cagtaagtta aaggctttct gatctccctt ctggacccgt tcaaccagga cctggtccgt
    68701 taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc gctatcgaaa
    68761 cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg tttttgtaaa
    68821 gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat ctatcattat
    68881 aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta actgcctgaa
    68941 gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag taacttctcg
    69001 ttttctcgcc gccctgaggc agcgtgttta gtcactgtaa caaatattaa aatagcaggt
    69061 gtttattcgc acaacatgat gttatgctga ccaaaccgtg tttagtaaat taaacaaaga
    69121 aaatgaatac tctccctgaa cattcatgtg acgtgttgat tatcggtagc ggcgcagccg
    69181 gactttcact ggcgctgcgc ctggctgacc agcatcaggt catcgttcta agtaaaggcc
    69241 cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg tttgatgaaa
    69301 ctgacagcat tgactcgcat gtggaagaca cattgattgc cggtgctggt atttgcgatc
    69361 gccatgcagt tgaatttgtc gccagtaatg cacgatcctg tgtgcaatgg ctaatcgacc
    69421 agggggtgtt gtttgatacc cacattcagc cgaatggcga agaaagctac catctgactc
    69481 gtgaaggtgg acatagtcac cgtcgtattc ttcatgccgc cgacgccacc ggtagagaag
    69541 tagaaaccac gctggtgggc aaggcgcaga accatccgaa tattcgcgtg ctggagcgca
    69601 gcaacgcggt tgatctgatt gtttctgaca aaattggcct gccgggcacg cgacgggttg
    69661 ttggcgcgtg ggtatggaac cgaaataaag aaacggttga aacctgccac gcaaaagcag
    69721 tggtactggc aaccggcggt gcttcaaaag tttatcagta caccaccaat ccggatattt
    69781 cttctggcga tggcattgct atggcgtggc gcgcaggctg ccggattgcc aatctcgaat
    69841 ttaatcagtt ccaccctacc gcgctgtatc acccacaggc acgcaatttc ctgttaacgg
    69901 aagcactgcg cggcgaaggc gcttatctca agcgcccgga tggcacgcgt tttatgcccg
    69961 attttgatga gcgtggcgaa ctggctccgc gcgatattgt cgcccgcgcc attgaccatg
    70021 agatgaaacg cctcggcgcg gattgtatgt tcctcgacat cagccataag cccgccgatt
    70081 ttattcgcca gcatttcccg atgatttatg aaaaattgct cgggctgggg atagatctca
    70141 cgaaagaacc cgtgccgatt gtgcctgctg cacattatac ctgcggtggt gtaatggttg
    70201 atgatcatgg gcgtacggac gtcgagggtt tgtatgccat tggcgaagtg agttataccg
    70261 gcttacacgg cgctaaccgc atggcctcga attcattgct ggagtgtctg gtctatggct
    70321 ggtcggcggc agaagatatc agcagacgta taccttatgc ccacggcgtc agtacgttac
    70381 cgccgtggga tgaaagccgc gttgagaacc ctgacgaacg ggtagtaatt cagcataact
    70441 ggcacgagct acgtctgttt atgtgggatt acgttggcat tgtgcgcaca acgaagcgcc
    70501 tggaacgcgc cctgcggcgg ataaccatgc tccaacaaga aatagacgaa tattacgccc
    70561 atttccgcgt ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt gccgagttga
    70621 ttgttcgctg tgcgatgatg cgcaaggaga gccgtggcct gcatttcacg ctggattatc
    70681 cggaactgct cacgcattcc ggtccatcga tcctttctgc cggcaatcat tacataaaca
    70741 gataaaaagc ctgggtcagc gccgtatacg cttcggaata gttctggtct ggcccacgaa
    70801 tgactaagcg atcgctaaag cattctcccg cctgcgggga gaatgccagc agcacccgat
    70861 gcggcagtcg cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc cagcccatac
    70921 ttaatgccag ttcagtaaaa ccattaccaa tctgctctgg cagcaccacg cagaaaaatc
    70981 cctcttcggt aatgcactcc gccgcacaag tcagcaacga tgggtgatca agcgtagtgg
    71041 tatagcgagc ctgttcccgt tgaggtgtcg cgcactctac tccctgctga tagtaaggtg
    71101 ggttgctgat gattaaatcg aagcgtactg tctgctgtgt gatccactgc agaatatccg
    71161 ccgtatggac gttaatccgc tctgcccacg gggactggtt gatattttcc tgcgcttgcg
    71221 cggcagcttc actttccagt tcaactgcat caatcatcac gctgtcatct gttcgctgcg
    71281 ccagcattaa tgccaacaac ccgctacccg cgccgatatc aaggcaacgt tttaccccag
    71341 ccacaggtgc ccatgcgccc agcaaaatgc catccgttcc cactttcatc gcacagcgat
    71401 cgtgagcaac aaaaaactgt ttaaaagtaa atccattacg acgaaacacg gatgtagact
    71461 gtgacatgaa aataaaacct tgcaggaaaa acggcgatag caccgggtga gaacaatacc
    71521 cgagaagcga tatccataca aacagatgaa gattgcagcc gtaacgtcta taatcagcgc
    71581 cccacacaga ggtagaacat gactgtaacg actttttccg aacttgaact cgacgaaagc
    71641 ctgctggaat ccctccagga taaaggtttc actcgcccga ccgccattca ggctgccgcc
    71701 attccgcctg cgctcgatgg ccgtgatgta ctcgggtctg cgccgacagg caccggtaaa
    71761 acggcggcgt atctgctgcc agcgttgcag cacctgctcg atttcccacg taagaaatcc
    71821 ggtccgccgc gtattttgat ccttaccccg actcgcgaac tggcgatgca ggtggccgat
    71881 catgcccgcg aactggcgaa acatacgcat ctggatattg ccaccatcac cggcggcgtg
    71941 gcctatatga accacgcaga agtgttcagc gaaaaccagg acattgtggt cgccacgacc
    72001 ggacgtctgc tgcaatacat aaaagaagag aacttcgatt gccgcgcggt tgaaacgctg
    72061 atcctcgacg aagcagaccg tatgctggat atgggcttcg cccaggatat cgaacatatt
    72121 gctggcgaaa cgcgctggcg taaacagacc ctgctctttt cggcaactct ggaaggcgat
    72181 gcgattcagg actttgccga gcgtctgctg gaagatccgg tggaggtttc tgccaatccg
    72241 tccacccgtg agcgcaaaaa aattcatcag tggtattacc gcgccgatga tcttgagcat
    72301 aaaaccgcgt tgctggtgca tctgttaaaa cagccggaag cgacccgctc aattgtgttt
    72361 gtgcgtaagc gtgagcgtgt gcatgagctg gcaaactggc tgcgcgaagc aggcatcaac
    72421 aactgctatc tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat taaacgcttg
    72481 accgaaggcc gcgtaaacgt actggttgct accgatgttg ccgcgcgcgg tatcgacatt
    72541 cctgacgtca gccacgtctt taacttcgat atgccgcgca gtggcgatac ttatttgcac
    72601 cgtatcggac gtaccgcgcg cgccggtcgt aaaggcaccg cgatttcgct ggtggaagcc
    72661 catgaccatc tgctgctggg caaagttggt cgctatattg aagagccaat taaagctcgc
    72721 gtaattgacg agttacgccc gaaaacgcgt gcgccaagcg agaagcagac cggcaagcca
    72781 tcgaagaaag tactggcaaa acgcgccgag aagaaaaaag ctaaagagaa agagaagccg
    72841 cgggtgaaaa aacgccatcg cgacaccaaa aatattggta agcgccgtaa accaagcgga
    72901 acgggcgtgc caccgcaaac gacagaagag taatctcaat gccgggttta agcccggcat
    72961 tgtttttatc ccggcaaaaa acactcttca acacactgaa taaaagtgtg catcgccggg
    73021 ctaaccgctt ttccggcatg gtgagcgcac attgccgtaa tggtctgcga ctgttcgcca
    73081 aaaggcaatt caattaattc tccgcactcc aactctttta ccaccgcaaa acgcggcagg
    73141 taactgactc cgatatttgc cgcgacacaa cgcttgatgc tttcgatact tataagctca
    73201 atggtgtttt ccaccgtgat ccgccgctgg cgcagcgtgc tctcaaatat ctgccggaag
    73261 acacattgtg gttcgttgat aataaagcta caggcgttat gtcttcccgg ctcagtaaaa
    73321 tcgacatctg caatttgcgg tgaagccacc agcgccagcg gttgttcacc caactctcgt
    73381 cggttcagag catcatcatt ccctacgcga taaaaaaccc cgacatcggc ctcatcattc
    73441 agtagcgcat cacggatcac gtaacagttc agcgactgca acgataaacg cacttttgga
    73501 gcgcgttgcc gaaaacgctg caacacctgc ggcattcgat aagagagcaa cgtttcgcct
    73561 gaaataacgc gtagttcccc gtccggatcc gactcttttt tagcagcctc acgaagcgta
    73621 tccatcactc gggtaagttc atagatgtgc ggcagcagct tttttccttc gcgggtaagg
    73681 cacattcgtc gaccaatttt ctcaaacaat tgtaccgaga actcctgctc aagctgctga
    73741 atatgaaaag tcaccgtcga ttgtgtgcag cacaattttt gcgaagctcg caaaaaggaa
    73801 ccctcttcca ccacggtttt aagtgtaata aaacggcgca gatccataac cccaaaccta
    73861 tcgaaaatat cgaatttaga atgtaaaaac attcattttt ttaaatgttc cgtgtcgggt
    73921 actgtctacc aaaacagagg agataacaag tgacaccgac ccttttaagt gctttttgga
    73981 cttacaccct gattaccgcc atgacgccag gaccgaacaa tattctcgcc cttagctctg
    74041 ctacgtcgca tggatttcgt caaagtaccc gtgtgctggc agggatgagt ctgggatttt
    74101 tgattgtgat gttactgtgt gcgggcattt cattttcact ggcagtgatt gacccggcag
    74161 cggtacacct tttgagttgg gcgggggcgg catatattgt ctggctggcg tggaaaatcg
    74221 ccaccagccc aacaaaggaa gacggacttc agacaaaacc aatcagtttt tgggccagct
    74281 ttgctttgca gtttgtgaac gtcaaaatca ttttgtacgg tgttacggca ctgtcgacgt
    74341 ttgttctgcc gcaaacacag gcgctaagct gggtagttgg cgtcagcgtt ttgctggcga
    74401 tgattgggac gtttggcaat gtgtgctggg cgctggcggg gcatctgttt cagcaattgt
    74461 ttcgccagta tggtcgccag ttaaatatcg tgcttgccct gttgctggtc tattgcgcgg
    74521 tacgcatttt ctattaacga aaaaaagcgg aagaggtcgc cctcttccgc ttagtaactt
    74581 gctacttaag ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc
    74641 cggagtcaga gagttaaagc gaactgcata accggataca cggatggtca gctgcggata
    74701 tttttccgga tgcttaactg catcttccag agtttcacga cgcagaacgt ttacgttcag
    74761 gtgttgacca ccttcaacgc gaacttctgg tttcacttct actggaactt cacggtactc
    74821 aatgtcaccc agtttgctta ctgcaaccac ttcatcttct gcataacctg cttttgcaac
    74881 gatgcaacgc gcttcgcctt tttcgctgtc cagcagccag aaagagttca gcagatcgtc
    74941 gttagcggct ttagtaatct ggatacctgt aatcatgtga tgcctccccg gcaaaattat
    75001 ttgatttgtt cagcctgtcg cggccaattg gtaaaaccat tgttgcttga gtgtatatat
    75061 actcctcaaa cacccttgaa tctttgattc aaatcaataa aaaccacaca tcaagtatgg
    75121 ttgcaaatgg attttattgt tttacatcaa cttatgcagg tgtgaaattt taccaattta
    75181 catttttttg cactcgttta agtctaaaaa atgagcatga ttttgttctg tagaaagaag
    75241 cagttaagct aggcggattg aagattcgcg ggagagcgag atggcaaacg aattaacctg
    75301 gcatgacgtg ctggctgaag agaagcaaca accctatttt cttaataccc tacagaccgt
    75361 cgccagcgag cggcagtccg gcgtcactat ctatccgcca caaaaggatg tctttaacgc
    75421 gttccgcttt acagcgttgg gtgacgttaa agtggtgatt ctcggccagg atccttatca
    75481 cggaccggga caggcgcatg gtctggcatt ttccgttcgc cccggcattg cgacaccgcc
    75541 gtcactgctg aatatgtata aagagctgga aaatactatt cccggtttca cccgccctaa
    75601 tcatggttat cttgaaagct gggcgcgtca gggcgttctg ctactcaata ctgtgttgac
    75661 ggttcgcgca ggtcaggcac attcccacgc cagcctcggc tgggagacct tcaccgataa
    75721 agtaatcagc ctgattaacc agcaccgcga aggcgtggtg tttttgttgt ggggctcaca
    75781 tgcgcaaaag aaaggggcga ttattgataa gcaacgccat cacatactga aagcgccgca
    75841 tccatcaccg ctttctgcgc atcgtgggtt ctttggctgc aaccattttg tgctggcaaa
    75901 tcagtggctg gaacaacgtg gcgagaaacc gattgactgg atgccagtat tactggcaga
    75961 aagtgagtaa atttgcagga aaacgccgga tggcagcgtt gccacccggc tgatttatca
    76021 ggctttattc tgacgccacc attcaccaag caaaacgccg gttgcaacag agatatttag
    76081 cccggcaacg ttgccagtac catcaatctt cacgcgcaaa tcgttcggat cgcgtgcggc
    76141 atccggtaac ccttcatatt cctgacccag caccagtacc attttcgctg gcagactggt
    76201 tttgaacagc ggtttcccct gctcgctgga agtggtcact acggtgtaac ctgcctgacg
    76261 gaaatcatcc agcacgttaa caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc
    76321 gccttctgcg gtacggatag ccgcccccga ctccagcagt gccgcatcct gcaccacaac
    76381 gcctttcacg ccgaagtgcg cgcagctgcg catcatgcca cccaggttat gcgggttaga
    76441 ttcgttttcc agcgccagta cacaatcctg cgcgcctgcc tggcttaccc actgctgcac
    76501 ggttgtaccg ttacgctttt tgatcaggaa gcaaacgccg ccgtgatgtt ccgtgcctga
    76561 cgcttttgtc agttccgctt catctaccac atggtacgct ttgcggtttg ctgccatcca
    76621 gcgcaaggct tctttaaaac gcggcgttac actctggata aaccaggcgc gaacaatcgc
    76681 ttccggacgg ctctggaaca gtgcctgaca ggcattttcg ccgtagacgc gggtttcttc
    76741 cgcacgctga cgacgcaaca cttccggatc aataaaactt ttaccactga tgccaccgtg
    76801 atcggccttt tccggcgtct catcacccgg cgcgcgggaa actgtgcgcc acggcgactc
    76861 ttcccacttg cggtcacggg gctgactctg tttgtcatcg cgggcggggc gacggccacc
    76921 gtcagcacga gattttcctg gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg
    76981 tttatcagaa tcatcatcac tgcggacata catcactttg accttgccgc ttttaccttt
    77041 catttcatcg ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga ttacccgaag
    77101 tgcgcgcggt tcgccatgat ttcgttccaa agcctgtgtc tatcataccc attgaataaa
    77161 acagattgtt gtctggaaca atgtccccga taatatgtaa catattagaa acataccggc
    77221 gtcgttgccg ataagtctcc ttactcatcc cgaggttagt tatgaatacc gtttgtaccc
    77281 attgtcaggc catcaatcgc attcccgacg atcggatcga agatgcggca aaatgcggac
    77341 gctgcggtca cgacttgttt gacggagagg tgattaatgc gaccggtgaa acgctcgaca
    77401 aattgctgaa agatgatcta cctgtggtga tcgacttctg ggcgccgtgg tgcggcccct
    77461 gccgtaactt cgcgccaatt tttgaagatg ttgcgcaaga gcgtagcggt aaagtgcgct
    77521 ttgtgaaagt gaataccgaa gctgaacgtg aattgagcag ccgctttgga attcgtagta
    77581 taccgacgat catgattttc aaaaatggtc aggttgtcga catgcttaat ggcgcagtac
    77641 cgaaagcgcc gttcgacagc tggctgaacg aatctcttta atcttaccgg ggcgcatctt
    77701 gtgccccgtt ttctcctctg cgacaatggc gttttttcga cgctctctta tgaccgaaaa
    77761 cgctgttctc cagttacgcg ccgagcgtat tgcgcgtgca acacgtcctt ttcttgcccg
    77821 cggtaatcgc gttcgtcgct gccaacgctg tcttttgcca gagaaattat gtctctgttc
    77881 gacaattacc ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc
    77941 aatgaagccc agtaataccg ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt
    78001 tcaatggtcg cgtaccgaac cctcgcaaga tttgctggat ctggtgcaaa acccggacta
    78061 tcagccaatg gtggtctttc ccgcttcgta tgctgatgag caacgggaag tgatcttcac
    78121 accacctgcc ggtaagcctc cgctatttat catgctcgat ggtacctggc cagaagctcg
    78181 caagatgttt cgtaaaagtc cgtatctgga taatcttccc gtcatttccg tcgatctttc
    78241 tcggctttct gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga
    78301 ggtagccatc gcactgttag atatggccgg cgataccagg gcggcgacag ggttaggcga
    78361 gcattttacc cgctttaaaa cacgctatct ggcaggaaaa acgcaacatc cgggaagcat
    78421 cacagcagaa cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg gagaccgata
    78481 tgagtcagcg aggactggaa gcactactgc gaccaaaatc gattgcggta attggcgcat
    78541 cgatgaaacc caatcgcgca ggttacctga tgatgcgtaa cctgctggcg ggaggcttta
    78601 acggaccggt actcccggtg acgccagcct ggaaagcggt gttgggtgtg ttggcctggc
    78661 cggatattgc ccgcttgccc tttacacccg accttgcggt tttatgtacc aatgccagcc
    78721 gtaatcttgc tcttctggaa gagctcggcg agaaaggctg taaaacctgc attattcttt
    78781 ccgccccggc atcgcaacac gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc
    78841 tgctaggacc aaacagtctg ggcttactgg ctccctggca aggtctgaat gccagctttt
    78901 cacctgtgcc gattaaacgc ggcaagctgg cgtttatttc gcaatcggct gctgtctcca
    78961 acaccatcct cgactgggcg caacagcgtg agatgggctt ttcctacttt attgcgctcg
    79021 gcgacagcct ggatatcgac gttgatgaat tgctcgacta tttggcgcgc gacagtaaaa
    79081 ccagcgccat cctgctctat ctcgaacagt taagcgacgc gcgacgcttt gtttcggcag
    79141 cccgtagtgc ctcgcgcaat aaaccgattt tggtgattaa aagcggacgt agcccggcgg
    79201 ctcaacgtct gttgaacacc acggcaggaa tggatccggc gtgggatgca gctattcagc
    79261 gtgccggttt gttgcgggta caggacaccc acgaactgtt ttcggcggtg gaaaccctta
    79321 gccatatgcg cccgctgcgt ggcgaccggt tgatgattat cagcaacggt gctgcgcctg
    79381 ccgcgctggc gctggatgcc ttatggtcac gcaatggcaa gctggcaacg ctaagcgaag
    79441 aaacctgcca gaaactgagc gatgcactgc cagggcatgt ggcaatctct aacccgctcg
    79501 atctgcgcga tgacgccagc agcgagcatt atgtcaaaac gctggacatt ctgctccaca
    79561 gccaggattt tgatgcgctg atggttattc attcgcccag cgccgctgct cccgcaacag
    79621 aaagcgcaca agtattaatt gaagcggtaa agcatcatcc ccgcagcaaa tatgtctctt
    79681 tgctgacgaa ctggtgcggc gagcactcct cgcaagaggc acgacgttta ttcagcgaag
    79741 ccgggctgcc gacctaccgt actccggaag gaaccatcac tgcttttatg catatggtcg
    79801 agtaccgacg taatcagaag caactacgcg aaacgccggc gttgcccagc aatctgactt
    79861 ccaataccgc agaagcgcat cttctgttgc aacaggcgat tgccgaaggg gctacgtcgc
    79921 tcgataccca tgaagttcag cccatcctgc aagcgtatgg tatgaatact ctccctacct
    79981 ggattgccag tgatagcacc gaagcggtac atattgccaa acagattggc tatccggtgg
    80041 cactgaaatt gcgctcgccg gatattccac ataaatcgga agttcagggc gtcatgctct
    80101 acctacgtac agccaatgaa gtccagcaag cggcaaacgc tattttcgat cgcgtaaaaa
    80161 tggcctggcc gcaggcgcgg gtccacggcc tgttggtgca aagtatggct aaccgtgctg
    80221 gcgctcagga gttgcgggtt gtggttgagc acgatccggt tttcgggccg ttaatcatgc
    80281 tgggtgaagg cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgca ctgccgccgc
    80341 tgaacatgaa cctggcccgc tatctggtta ttcaggggat caaaagtaaa aagattcgtg
    80401 cacgcagtgc gctacgccca ttggatgttg caggcttgag ccagcttctg gtgcaggttt
    80461 ccaacttgat tgtcgattgc ccggaaattc agcgtctgga tattcatcct ttgctggctt
    80521 ctggcagtga atttaccgcg ctggatgtca cgctggatat cgcgccgttt gaaggtgata
    80581 acgagagccg gctggctgtg cgcccttatc cgcatcagct ggaagaatgg gtagaattga
    80641 aaaacggtga acgctgcttg ttccgcccga ttttgccaga agatgagcca cagcttcagc
    80701 aattcatttc gcgagtcacc aaagaagatc tttattaccg ctactttagc gagatcaacg
    80761 aatttaccca tgaagattta gccaacatga cgcagatcga ctacgatcgg gaaatggcat
    80821 ttgtagcggt acgacgaatt gatcaaacgg aagagatcct cggcgtcaca cgtgcgatct
    80881 ccgaccctga taacatcgat gccgaatttg ccgtgctggt tcgctcggat ctcaaagggt
    80941 taggcttagg tcgacgctta atggaaaagt tgattaccta tacgcgagat cacggactac
    81001 aacgtctgaa tggtattacg atgccaaaca atcgtggcat ggtggcgctg gcccgcaagc
    81061 tcgggtttaa cgttgatatc cagctcgaag aggggatcgt tgggcttacg ctaaatcttg
    81121 cccagcgcga ggaatcatga gtaaggtact ggaaatgttg accacttaat cgggactggt
    81181 gttattattg cccgcttatg tcgtctgcat tgcacagagg acccttcaat gaacagagaa
    81241 gaaatgcact gtgatgttgt caaaatttaa gcgtaataaa catcaacaac accttgccca
    81301 actacccaag atttctcaat cagttgatga tgtcgatttc ttttacgctc ccgccgactt
    81361 ccgggagacg ctgctggaaa aaatagccag cgcgaagcag cgcatttgca ttgttgccct
    81421 gtatctcgaa caggatgacg gtggcaaagg cattctgaac gcgttgtatg aggctaaaag
    81481 gcagcgtccg gaactggatg tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg
    81541 cattggcgct gcggcatcta acactaacgc tgactggtac tgccgcatgg cgcaggaaaa
    81601 tccgggcgta gatgttccgg tttatggcgt tccaatcaat acccgtgaag cccttggtgt
    81661 tctgcacttt aaaggcttta tcatcgacga tagcgtactt tatagcggtg ccagcctgaa
    81721 cgatgtttac ctgcatcagc acgataaata tcgctacgac cgttatcatc tgatccgtaa
    81781 ccgtaagatg tcagacatta tgtttgaatg ggttacacag aatattatga atggccgcgg
    81841 cgttaatcgt ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga acgacattcg
    81901 cctgttccgc caggagctgc gtgatgccgc ttatcatttc cagggcgatg ccgacaacga
    81961 tcagctttcc gtaacgccgc tagtggggct ggggaaatcg agtctgttga acaagaccat
    82021 tttccatctt atgccttgtg ccgagcagaa actaaccatc tgtacgccat acttcaacct
    82081 gccagcaatc cttgtgcgca atattatcca gttgctgcgc gaagggaaaa aggtcgaaat
    82141 tattgttggt gataaaaccg cgaatgactt ctacattccg gaagatgaac ctttcaagat
    82201 aattggcgca ttgccttatc tctatgagat caatctgcgt cgtttcctga gtcgtttaca
    82261 gtattacgtc aacactgacc agttagtagt tcggttatgg aaagatgacg acaacaccta
    82321 tcacctgaaa gggatgtggg ttgatgataa gtggatgttg atcaccggta ataacctgaa
    82381 cccgcgcgcc tggcgtctgg atctggaaaa cgccattttg atccacgatc cgcaactgga
    82441 gcttgcgcca cagcgagaga aagaactgga tctgatccgc gagcatacca ccatcgttaa
    82501 gcactaccgc gatctgcaaa gtattgccga ttatccggtg aaggttcgta aactcatccg
    82561 ccgtttgcgc cgtatccgca tcgaccgatt aattagccgc atcctgtaat cacaaccccg
    82621 tcctgtacgg ggtttgtttt ttggaggcca cgttttgcgt attctttttg tctgttcgct
    82681 attgttactt tctggatgca gccatatggc taacgataac tggagcgggc aggataaagc
    82741 tcaacacttt atcgcctcgg cgatgctttc cgccgccgga aatgaatatt cacagcatca
    82801 ggggatgagc cgggatcgca gtgccatgtt tggattgatg ttctctgtca gtctgggggc
    82861 gtcaaaagag ctttgggata gccgccctga ggggagcggc tggagctgga aggattttgc
    82921 ctgggatatc gccggtgcaa gcaccggcta taccgtctgg caactgaccc gtcactaaag
    82981 ccgcatccct tttcctttac ggtgcaatgt tagtgaaacc agaaacgcca ccacggccat
    83041 caaggtcaca taccagaaga aggctgtttc cattcctatt gatttcagcg acaacgctac
    83101 gtactccgcc gaaccaccaa atatagcatt ggcgaccgca tatgacagac caacgcctaa
    83161 tgcgcgaacc tgtgccggga acatctcagc cttcagtatt ccactgattg aagtataaaa
    83221 actcactatc agcagggcgc acatcaccag accaaaagcg gcataaggcg aggaaacgtt
    83281 ctgcaatgct gagagaatag gaacggtaaa aatggttgcc agagaaccga aacataacat
    83341 tgaggtacgg cgaccaatct tatccgacag cgcgccaata agtggttgaa taagcatgaa
    83401 tacaaacaat gcggcagtca taatgccact cgccacgttg gcatgcattc ccgcagtatt
    83461 taccagatac ttctgcatat aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt
    83521 aaaaccgaga accatgatga atgcacggcg attgcgccat aatcctttca gagatccagc
    83581 ttcttttaaa gcgcgtgttt cttgttgtga agtttcatct aactgacgac gtaaccacaa
    83641 cgccacaaca gctaacacag cccctaacgc gaaaggaata cgccatcccc actctctgag
    83701 tacagcgtct tccatggtgt gttgtaaaac cacgacaacc agtagagcta gcagttgccc
    83761 gccgatcaac gtcacatact gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac
    83821 ttcactcata taggtggcgc tggtgccata ttctccgcca acggataatc cctggaataa
    83881 acgcgcgaga agcaataatg ccggagccca ggtaccgatt gtttcgtagc ctggcaaaca
    83941 ggcgataacc agtgagccga aacacatcat acacaccgat aacagcatcg attttttgcg
    84001 accatgttta tcggctatgc ggccaaacag ccaaccgcct attgggcgca tcagaaatcc
    84061 tgcagcaaaa acacctgctg tttgcagtag ttgagtcgtc gtgttcccgg aagggaagaa
    84121 gatgtgggcg aagtagagtg aacagaacga gtagacatag aaatcgaacc actcgaccag
    84181 attacctgaa gaggccccaa caattgccta aatgcggcga cgagtatcac tacttgtcag
    84241 tttgctgtct gccgttacag tactttcagc catgccatta tgtctcctgc cgtaatccaa
    84301 tgcttttgtc ggtcgctttt gtttattttt tggtaagaaa aaatgttata catttgttgc
    84361 atatcattat gcaaccttaa ccatgaattt agttagcagg aaaatggtta ttaaggagct
    84421 taaggataga tttctggtaa ggaggaagta tggaagtggg caatttgggg aagacgtatt
    84481 cgctgttgaa tctggcttat gtgggagcgt agatactgtt acggccagca agttttttcc
    84541 tggtccagtc tcccggattg caggcatgcc agacacagcg ccgtcaggcg ctgttgtttc
    84601 atcccttccg cgatttttac gcagctaccg gattatgccg tgacgcatcg aacggcacgc
    84661 ctgacttcag cactccatac gccacctgtg ccagcttgcg cattatcgag ccgagaatca
    84721 cctttccttt cttgccatta gctgccaacg cccgtcccca ctcagtctta ctggtggcta
    84781 ccattgcagg catatacaac ggcctgcgaa gcgacacatg tccggcttta ctcaaccggc
    84841 ttgcccctct cacactactg cctgattcat aacgccgtgg tgtcagaccc gcaaaagcgg
    84901 cgaactgtct ggcatgggca aaacggtcct tcagaccatg taagccagca ataccgcaga
    84961 tgttttctct ccgatacccg ggatgctttc cagcagtttc ctgcggtgtt tcatatccgg
    85021 atcatcgtct gtcaggtctt ttatctgctt ctcaagacgc ttcagctctg cttcaagcca
    85081 cagaaggtga gcatcaatgc tcggcctctg gacttcccgc gccgtttcag tgcgattcag
    85141 ttcctgcctg tgcatatctg tcagcgcctg gtggcgtact accagggcat gcaacgcgcg
    85201 ttcaagcggg tgatgcgctt cccaggctgc agggcgcttc tgacgacaga actctgccag
    85261 catgcggcat ccacggtatc agtcttgtta cgcagtcctt cactctgagc gaaagcttta
    85321 cccagtgcag gattaatgac tgacactatg tagccagcat cgtaaaggca ctctgcgaca
    85381 ggttccatat aggtgccggt cgcttcgatg cagatatgcg catggtcaat cttgtgacat
    85441 ttcagccaac tcaccagctc atcgtgccct ttagtggtgt tagcgaattt tttggtgcga
    85501 tgacgaccat caggacgcaa cacatcgaca tccagtttct ctttagcggt gtcgataccg
    85561 atataatgaa gttcatgttc catagtgaaa ccaaccttgc aaatacggat taccgggaaa
    85621 accggtctat gatactgtcc ggtttatcac ttcgggagaa aggcagtccg ccgcataaat
    85681 ctaccatgca ggttagaacc caaggccgga tacggcatgc ggactgcagg cagagaaaac
    85741 tggccgattt tctctgcact ggagggataa tacaaggtcg gataagatgc ggcatgaact
    85801 gaaccccaaa acctggacag tttcagttaa ccggcttttt gatactcagt ccggtattct
    85861 accgggctga gtcccccaag actgagtttt attctttttt gattccagta agttatgtat
    85921 tcgttaacgg cattttccag ctcttctaca tttctgtagt cccggcgata gtacatttct
    85981 tctttcaggt gaccaaagaa gttctccatt accgcattat caaggcagtt gcccttgcgc
    86041 gacatgctct gcactaaccc tctgtccgcc agggcggact gataactttt tatccggtaa
    86101 tgccatcctt gatcactgtg gagcagcggt ttctcccctt cagcaaggct ttccagcccc
    86161 ttatttaaca tccgcttcac cagctcctct gtaggcctgc aggccgtttc ccacgccacg
    86221 atttccccgt taaacaaatc aagtatcggg gacaggtaca gtttttgtcc gccagccctg
    86281 aattcggtga tatctgtcac ccatttctca cagggagctt ccgctttgaa ctgtcgctga
    86341 aggatatttt ctgcagcgag tcccatattt ccccggtaag accggtattt cttcaggcgc
    86401 acaggagact tcaggccgag ctgttgcatc agttttctca ccgttttgcc actgaactta
    86461 agacctcttt tctgaagctc acaatggatc cgccggtacc cataacatcc ccggtgttca
    86521 tggaagatgg aggctatgag ctgtttcaca tcagcatact tgtccttagc cttctgcagt
    86581 gacagctggt agtacagcgt gcttctggcc agccctgcgg cctttaacag gtcagactgg
    86641 cagtgtccgt acctcaggct ctggatgact ttcgtttttt ctccagctcc tggcgctttt
    86701 tttccagaag atgctcctgc atggctttca gatatgcatt ttctgcgcga agataacgca
    86761 gctcattctt catctcttcc ggcgtcatgt ttgcctcgtt ttcatgggta tcatcagaac
    86821 gggtcatgct tttctccttc ctgcgcctga taaaggcctc attaccagat ttgcggtagc
    86881 cttttatcca gtttttaacc gtgttgtggc tgggaatgtt aaaccgggca gctacccggg
    86941 gtagagactc agaatgcccg agagcataaa gaaccacgca aagtttatct tcaggagaat
    87001 agctacgctt gtgtctgcaa tccagtgccg aggaccgtga agaagaaaaa gattaatcca
    87061 gtgtgagagg ctggtgtgag aaagttgaaa gagttttgcc gtagaaatga tgcctgcatg
    87121 accagccagg taatgattta cagcggcaag ttttatttca aatgaatgtt tcataaactg
    87181 caccttcaga tgtcagatta tgtccaacat ttggggtgca gttca
//
LOCUS       DAZFRE010000006        82778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFRE010000006 DAZFRE010000000
VERSION     DAZFRE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.006"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..303)
                     /locus_tag="ACHKUI_000491"
     CDS             complement(<1..303)
                     /locus_tag="ACHKUI_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6596755.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
     gene            complement(568..828)
                     /gene="mcbA"
                     /locus_tag="ACHKUI_000492"
     CDS             complement(568..828)
                     /gene="mcbA"
                     /locus_tag="ACHKUI_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO6596756.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            1104..2030
                     /gene="rlmF"
                     /locus_tag="ACHKUI_000493"
     CDS             1104..2030
                     /gene="rlmF"
                     /locus_tag="ACHKUI_000493"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6596757.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(2027..4252)
                     /gene="ybiO"
                     /locus_tag="ACHKUI_000494"
     CDS             complement(2027..4252)
                     /gene="ybiO"
                     /locus_tag="ACHKUI_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6596758.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(4369..5091)
                     /gene="glnQ"
                     /locus_tag="ACHKUI_000495"
     CDS             complement(4369..5091)
                     /gene="glnQ"
                     /locus_tag="ACHKUI_000495"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6596759.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(5088..5747)
                     /gene="glnP"
                     /locus_tag="ACHKUI_000496"
     CDS             complement(5088..5747)
                     /gene="glnP"
                     /locus_tag="ACHKUI_000496"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6596760.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(5886..6632)
                     /gene="glnH"
                     /locus_tag="ACHKUI_000497"
     CDS             complement(5886..6632)
                     /gene="glnH"
                     /locus_tag="ACHKUI_000497"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6596761.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(7036..7539)
                     /gene="dps"
                     /locus_tag="ACHKUI_000498"
                     /gene_synonym="pexB"
     CDS             complement(7036..7539)
                     /gene="dps"
                     /locus_tag="ACHKUI_000498"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6596762.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(7839..8726)
                     /gene="rhtA"
                     /locus_tag="ACHKUI_000499"
     CDS             complement(7839..8726)
                     /gene="rhtA"
                     /locus_tag="ACHKUI_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6596763.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8961..9026
                     /locus_tag="ACHKUI_000500"
     CDS             8961..9026
                     /locus_tag="ACHKUI_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596764.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            9079..9594
                     /gene="ompX"
                     /locus_tag="ACHKUI_000501"
     CDS             9079..9594
                     /gene="ompX"
                     /locus_tag="ACHKUI_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6596765.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(9643..11226)
                     /gene="opgE"
                     /locus_tag="ACHKUI_000502"
     CDS             complement(9643..11226)
                     /gene="opgE"
                     /locus_tag="ACHKUI_000502"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6596766.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDNFDPKP"
     gene            complement(11498..11626)
                     /gene="mntS"
                     /locus_tag="ACHKUI_000503"
     CDS             complement(11498..11626)
                     /gene="mntS"
                     /locus_tag="ACHKUI_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6596767.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            11812..12279
                     /gene="mntR"
                     /locus_tag="ACHKUI_000504"
     CDS             11812..12279
                     /gene="mntR"
                     /locus_tag="ACHKUI_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6596768.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            12276..13394
                     /gene="ybiR"
                     /locus_tag="ACHKUI_000505"
     CDS             12276..13394
                     /gene="ybiR"
                     /locus_tag="ACHKUI_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6596769.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(13452..14372)
                     /gene="ldtB"
                     /locus_tag="ACHKUI_000506"
     CDS             complement(13452..14372)
                     /gene="ldtB"
                     /locus_tag="ACHKUI_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6596770.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            14591..16183
                     /gene="ybiT"
                     /locus_tag="ACHKUI_000507"
     CDS             14591..16183
                     /gene="ybiT"
                     /locus_tag="ACHKUI_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6596771.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(16383..17198)
                     /gene="ybiV"
                     /locus_tag="ACHKUI_000508"
     CDS             complement(16383..17198)
                     /gene="ybiV"
                     /locus_tag="ACHKUI_000508"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6596772.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(17344..19776)
                     /gene="ybiW"
                     /locus_tag="ACHKUI_000509"
     CDS             complement(17344..19776)
                     /gene="ybiW"
                     /locus_tag="ACHKUI_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO6596773.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSHINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(19782..20681)
                     /gene="ybiY"
                     /locus_tag="ACHKUI_000510"
     CDS             complement(19782..20681)
                     /gene="ybiY"
                     /locus_tag="ACHKUI_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6596774.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCSWCQNPESRARTQDL
                     LYDPRLCLEGCKLCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            20812..21474
                     /gene="fsa"
                     /locus_tag="ACHKUI_000511"
     CDS             20812..21474
                     /gene="fsa"
                     /locus_tag="ACHKUI_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6596775.1"
                     /translation="MELYLDTSDVVAVTALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            complement(21550..22299)
                     /gene="moeB"
                     /locus_tag="ACHKUI_000512"
     CDS             complement(21550..22299)
                     /gene="moeB"
                     /locus_tag="ACHKUI_000512"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6596776.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKLPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(22299..23534)
                     /gene="moeA"
                     /locus_tag="ACHKUI_000513"
     CDS             complement(22299..23534)
                     /gene="moeA"
                     /locus_tag="ACHKUI_000513"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6596777.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALHAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VDVGEWVEVEPFNALFGGL"
     gene            23738..24703
                     /gene="iaaA"
                     /locus_tag="ACHKUI_000514"
     CDS             23738..24703
                     /gene="iaaA"
                     /locus_tag="ACHKUI_000514"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6596778.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYRERGDTVATQ"
     gene            24690..26561
                     /gene="gsiA"
                     /locus_tag="ACHKUI_000515"
     CDS             24690..26561
                     /gene="gsiA"
                     /locus_tag="ACHKUI_000515"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6596779.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            26581..28119
                     /gene="gsiB"
                     /locus_tag="ACHKUI_000516"
     CDS             26581..28119
                     /gene="gsiB"
                     /locus_tag="ACHKUI_000516"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6596780.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFVGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKKAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            28137..29057
                     /gene="gsiC"
                     /locus_tag="ACHKUI_000517"
     CDS             28137..29057
                     /gene="gsiC"
                     /locus_tag="ACHKUI_000517"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO6596781.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            29060..29971
                     /gene="gsiD"
                     /locus_tag="ACHKUI_000518"
     CDS             29060..29971
                     /gene="gsiD"
                     /locus_tag="ACHKUI_000518"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6596782.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            30148..32496
                     /gene="pdeI"
                     /locus_tag="ACHKUI_000519"
     CDS             30148..32496
                     /gene="pdeI"
                     /locus_tag="ACHKUI_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6596783.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRV
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            32504..33832
                     /gene="dgcI"
                     /locus_tag="ACHKUI_000520"
     CDS             32504..33832
                     /gene="dgcI"
                     /locus_tag="ACHKUI_000520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6596784.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(33879..35204)
                     /gene="rimO"
                     /locus_tag="ACHKUI_000521"
     CDS             complement(33879..35204)
                     /gene="rimO"
                     /locus_tag="ACHKUI_000521"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6596785.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            35417..35800
                     /gene="bssR"
                     /locus_tag="ACHKUI_000522"
     CDS             35417..35800
                     /gene="bssR"
                     /locus_tag="ACHKUI_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6596786.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            35911..37026
                     /gene="yliI"
                     /locus_tag="ACHKUI_000523"
     CDS             35911..37026
                     /gene="yliI"
                     /locus_tag="ACHKUI_000523"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6596787.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(37023..37649)
                     /gene="gstB"
                     /locus_tag="ACHKUI_000524"
     CDS             complement(37023..37649)
                     /gene="gstB"
                     /locus_tag="ACHKUI_000524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6596788.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            37895..39097
                     /gene="dacC"
                     /locus_tag="ACHKUI_000525"
     CDS             37895..39097
                     /gene="dacC"
                     /locus_tag="ACHKUI_000525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6596789.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(39144..39902)
                     /gene="deoR"
                     /locus_tag="ACHKUI_000526"
     CDS             complement(39144..39902)
                     /gene="deoR"
                     /locus_tag="ACHKUI_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6596790.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLIEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(39960..40556)
                     /gene="ybjG"
                     /locus_tag="ACHKUI_000527"
     CDS             complement(39960..40556)
                     /gene="ybjG"
                     /locus_tag="ACHKUI_000527"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6596791.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            40841..42073
                     /gene="mdfA"
                     /locus_tag="ACHKUI_000528"
     CDS             40841..42073
                     /gene="mdfA"
                     /locus_tag="ACHKUI_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6596792.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(42114..42398)
                     /gene="ybjH"
                     /locus_tag="ACHKUI_000529"
     CDS             complement(42114..42398)
                     /gene="ybjH"
                     /locus_tag="ACHKUI_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6596793.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(42484..43299)
                     /gene="ybjI"
                     /locus_tag="ACHKUI_000530"
     CDS             complement(42484..43299)
                     /gene="ybjI"
                     /locus_tag="ACHKUI_000530"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6596794.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            complement(43299..44507)
                     /gene="ybjJ"
                     /locus_tag="ACHKUI_000531"
     CDS             complement(43299..44507)
                     /gene="ybjJ"
                     /locus_tag="ACHKUI_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6596795.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            44591..45127
                     /gene="rcdA"
                     /locus_tag="ACHKUI_000532"
     CDS             44591..45127
                     /gene="rcdA"
                     /locus_tag="ACHKUI_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6596796.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(45302..46987)
                     /gene="ybjL"
                     /locus_tag="ACHKUI_000533"
     CDS             complement(45302..46987)
                     /gene="ybjL"
                     /locus_tag="ACHKUI_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6596797.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            47257..47634
                     /gene="ybjM"
                     /locus_tag="ACHKUI_000534"
     CDS             47257..47634
                     /gene="ybjM"
                     /locus_tag="ACHKUI_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6596798.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(47664..47921)
                     /gene="grxA"
                     /locus_tag="ACHKUI_000535"
     CDS             complement(47664..47921)
                     /gene="grxA"
                     /locus_tag="ACHKUI_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6596799.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            48081..48368
                     /gene="ybjC"
                     /locus_tag="ACHKUI_000536"
     CDS             48081..48368
                     /gene="ybjC"
                     /locus_tag="ACHKUI_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6596800.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMIPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            48352..49074
                     /gene="nfsA"
                     /locus_tag="ACHKUI_000537"
     CDS             48352..49074
                     /gene="nfsA"
                     /locus_tag="ACHKUI_000537"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6596801.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            49135..50037
                     /gene="rimK"
                     /locus_tag="ACHKUI_000538"
     CDS             49135..50037
                     /gene="rimK"
                     /locus_tag="ACHKUI_000538"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6596802.1"
                     /translation="MKIAILSRDGTLYSCKRLREVAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            50125..50601
                     /gene="ybjN"
                     /locus_tag="ACHKUI_000539"
     CDS             50125..50601
                     /gene="ybjN"
                     /locus_tag="ACHKUI_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6596803.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            50951..52063
                     /gene="potF"
                     /locus_tag="ACHKUI_000540"
     CDS             50951..52063
                     /gene="potF"
                     /locus_tag="ACHKUI_000540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6596804.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMVF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            52158..53291
                     /gene="potG"
                     /locus_tag="ACHKUI_000541"
     CDS             52158..53291
                     /gene="potG"
                     /locus_tag="ACHKUI_000541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6596805.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            53301..54254
                     /gene="potH"
                     /locus_tag="ACHKUI_000542"
     CDS             53301..54254
                     /gene="potH"
                     /locus_tag="ACHKUI_000542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6596806.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            54251..55096
                     /gene="potI"
                     /locus_tag="ACHKUI_000543"
     CDS             54251..55096
                     /gene="potI"
                     /locus_tag="ACHKUI_000543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6596807.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            55156..55644
                     /gene="ybjO"
                     /locus_tag="ACHKUI_000544"
     CDS             55156..55644
                     /gene="ybjO"
                     /locus_tag="ACHKUI_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO6596808.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            55685..56812
                     /gene="rlmC"
                     /locus_tag="ACHKUI_000545"
     CDS             55685..56812
                     /gene="rlmC"
                     /locus_tag="ACHKUI_000545"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6596809.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTVDLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLTQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLILLVKQ"
     gene            complement(56841..57572)
                     /gene="artJ"
                     /locus_tag="ACHKUI_000546"
     CDS             complement(56841..57572)
                     /gene="artJ"
                     /locus_tag="ACHKUI_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6596810.1"
                     /translation="MKKLVLAALLTSFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(57798..58466)
                     /gene="artM"
                     /locus_tag="ACHKUI_000547"
     CDS             complement(57798..58466)
                     /gene="artM"
                     /locus_tag="ACHKUI_000547"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6596811.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(58466..59182)
                     /gene="artQ"
                     /locus_tag="ACHKUI_000548"
     CDS             complement(58466..59182)
                     /gene="artQ"
                     /locus_tag="ACHKUI_000548"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6596812.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAVIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(59189..59920)
                     /gene="artI"
                     /locus_tag="ACHKUI_000549"
     CDS             complement(59189..59920)
                     /gene="artI"
                     /locus_tag="ACHKUI_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6596813.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(59938..60666)
                     /gene="artP"
                     /locus_tag="ACHKUI_000550"
     CDS             complement(59938..60666)
                     /gene="artP"
                     /locus_tag="ACHKUI_000550"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6596814.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(60884..61399)
                     /gene="ybjP"
                     /locus_tag="ACHKUI_000551"
     CDS             complement(60884..61399)
                     /gene="ybjP"
                     /locus_tag="ACHKUI_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6596815.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            61525..61848
                     /gene="ybjQ"
                     /locus_tag="ACHKUI_000552"
     CDS             61525..61848
                     /gene="ybjQ"
                     /locus_tag="ACHKUI_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6596816.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            61845..62675
                     /gene="amiD"
                     /locus_tag="ACHKUI_000553"
     CDS             61845..62675
                     /gene="amiD"
                     /locus_tag="ACHKUI_000553"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6596817.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(62672..63685)
                     /gene="ybjS"
                     /locus_tag="ACHKUI_000554"
     CDS             complement(62672..63685)
                     /gene="ybjS"
                     /locus_tag="ACHKUI_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6596818.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQGELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(63784..65214)
                     /gene="ybjT"
                     /locus_tag="ACHKUI_000555"
     CDS             complement(63784..65214)
                     /gene="ybjT"
                     /locus_tag="ACHKUI_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6596819.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPALLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(65225..66226)
                     /gene="ltaE"
                     /locus_tag="ACHKUI_000556"
     CDS             complement(65225..66226)
                     /gene="ltaE"
                     /locus_tag="ACHKUI_000556"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6596820.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAASDGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNATWMAEQLREAGADVMRQDTN
                     MQFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWHAFLAR"
     gene            complement(66263..67981)
                     /gene="poxB"
                     /locus_tag="ACHKUI_000557"
     CDS             complement(66263..67981)
                     /gene="poxB"
                     /locus_tag="ACHKUI_000557"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6596821.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERNFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(68114..69082)
                     /gene="hcr"
                     /locus_tag="ACHKUI_000558"
     CDS             complement(68114..69082)
                     /gene="hcr"
                     /locus_tag="ACHKUI_000558"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6596822.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(69094..70746)
                     /gene="hcp"
                     /locus_tag="ACHKUI_000559"
                     /gene_synonym="priS"
     CDS             complement(69094..70746)
                     /gene="hcp"
                     /locus_tag="ACHKUI_000559"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6596823.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLMSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(70890..71789)
                     /gene="lysO"
                     /locus_tag="ACHKUI_000560"
     CDS             complement(70890..71789)
                     /gene="lysO"
                     /locus_tag="ACHKUI_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6596824.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(72110..72805)
                     /gene="aqpZ"
                     /locus_tag="ACHKUI_000561"
     CDS             complement(72110..72805)
                     /gene="aqpZ"
                     /locus_tag="ACHKUI_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6596825.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            73231..74889
                     /gene="ybjD"
                     /locus_tag="ACHKUI_000562"
     CDS             73231..74889
                     /gene="ybjD"
                     /locus_tag="ACHKUI_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6596826.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(74886..75842)
                     /gene="ybjX"
                     /locus_tag="ACHKUI_000563"
     CDS             complement(74886..75842)
                     /gene="ybjX"
                     /locus_tag="ACHKUI_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6596827.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            75993..77108
                     /gene="macA"
                     /locus_tag="ACHKUI_000564"
     CDS             75993..77108
                     /gene="macA"
                     /locus_tag="ACHKUI_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6596828.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            77105..79051
                     /gene="macB"
                     /locus_tag="ACHKUI_000565"
     CDS             77105..79051
                     /gene="macB"
                     /locus_tag="ACHKUI_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6596829.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNIIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(79124..79348)
                     /gene="cspD"
                     /locus_tag="ACHKUI_000566"
     CDS             complement(79124..79348)
                     /gene="cspD"
                     /locus_tag="ACHKUI_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6596830.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            79498..79572
                     /gene="yljB"
                     /locus_tag="ACHKUI_000567"
     CDS             79498..79572
                     /gene="yljB"
                     /locus_tag="ACHKUI_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6596831.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            79671..79991
                     /gene="clpS"
                     /locus_tag="ACHKUI_000568"
     CDS             79671..79991
                     /gene="clpS"
                     /locus_tag="ACHKUI_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6596832.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSIYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            80022..82298
                     /gene="clpA"
                     /locus_tag="ACHKUI_000569"
     CDS             80022..82298
                     /gene="clpA"
                     /locus_tag="ACHKUI_000569"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6596833.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTLYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT        20232 a        20358 c        21893 g        20295 t
ORIGIN      
        1 cacgccgggg acgtttttga gcgcgtcggt aaggttggtt gcaccctgat ctttaatcac
       61 ttgttcagaa atcactgtca tcgtgcgggt agtatccgct accggacgcg agaatttcgg
      121 atcggcagat tgttgtggcg cataaagcga aggcgttgat gcttcgacaa ccagcgtgtc
      181 atccgcgtta gcttgccctt cggcagcgag tgcctgcgcc acaggcgtaa taccaataca
      241 aagaccggca aagaacgtga gcgaatgaaa ttgtctggca gggaaattgc gattgttttc
      301 cattttgcag gtgacttttt cttatatggt gaaattttca ctgtcacgag aaagcatctc
      361 tgcggatgcg atgccactgt taatcccgtg atagcgtccg tgcaagtgat aataattttg
      421 ataagcatta tcacttggcg cgattttcta tcacccattg cgcgaaaaag aaatacattt
      481 atttacatta cgttcatatt tgatgttttt tgcttacaaa ttggacgaaa actgcatcca
      541 gaggcgaatg cagttacgtt tgtggggtta tttgtagatg attgcagtac cgaacatctg
      601 gtcatttccg cctgcggaat taatgacata acctttcgcg ccttgttcgc gcgctttttc
      661 tgccagttta tcctccagat cgcttaggtt tgatgcgcca gttgccgaaa cggtaccggc
      721 gggccgtagt tgaccactgt ccaggttact catcggttgc gcggcataag ccgtgagaga
      781 gattccgctc aggacggtgg caatcagtag tgtgaggcac tttttcataa tgacatcctc
      841 ataaatacaa cgggtagtgc cagataagtg taggtcgcct gcgcgatgaa aatgacgcaa
      901 aaaattgaca gagatgcgct tattttttga acgatatgtt tttacaaaat aagacaaatc
      961 aatgacatgc atgatgatga tttttatggt gaattgccat aaaccatagc catgtgaagg
     1021 tttttagaaa tgataaacgc agccagatat ttaccctgtc gcgttatgcg ggtatcttac
     1081 gccgctgctt aaaggagaat gctatgtccg cccagaaacc ggggttgcat ccgcgcaacc
     1141 gtcatcacag ccgctacgat ctcgccacgc tttgtcaggt caatcctgaa ctcaggcaat
     1201 tcctcacgct tacacccgcc ggggagcaaa gcgtagactt tgccaatccg ctggcggtga
     1261 aggcgctcaa taaggcgttg ctggcccatt tttacgccgt agcgaactgg gatatcccgg
     1321 acggttttct ctgcccgcca gtaccgggcc gggcggatta tattcatcac cttgccgatt
     1381 tactggctga agcaagcgga acaattccag ctaatgccag cattctggat atcggcgttg
     1441 gtgcgaactg tatttatccg ctgattggcg tacatgaata tggctggcgt tttaccggta
     1501 gtgaaaccag cagccaggcg ttaagcagtg cgcaggcgat catcagtgct aatccggggc
     1561 ttaaccgcgc tattcgtctg cgtcggcaaa aagagagtgg ggcgattttt aacggcatca
     1621 tccataaaaa cgagcaatac gatgcgacct tgtgtaaccc gccattccac gattccgccg
     1681 ctgcggcacg ggcaggtagt gaacgtaaac gccgtaacct ggggctgaac aaagacgatg
     1741 cactgaactt tggtggtcag caacaggagt tgtggtgtga aggcggtgag gtcgctttta
     1801 tcaaaaagat gattgaagag agcaaaggct tcgcgaagca ggtgatgtgg tttacctcgc
     1861 tggtatctcg tggtgaaaac ttaccgccgt tgtatcgtgc tctgacggac gtaggtgcgg
     1921 tgaaggtggt aaaaaaagag atggcccagg ggcaaaagca gagccgcttt atagcctgga
     1981 cctttatgaa cgacgagcag cgccgccgtt ttgtcaatcg ccagcgttaa agcgttggct
     2041 cccccggcgg caacggttca gccggggccg cgcccggagc tggcagcacc tgataagtct
     2101 gcaccggcgc gcgaacaccc gccaggtcga aatgtttttt cacctggctg tcgagggcaa
     2161 agcggaccgt ccactgtttg agtggcagcg tggtgaacga aacacgcagt gtaaacgcgg
     2221 tattgcttaa gccgacaatc ccggcaaagt tcggttcacc aataaccagc ccgcgaattt
     2281 cttcgttttc catcaattct gctaccgcat ctttcagtgc ctgattggct ttatcagcat
     2341 cttcatggcg atcaacatcg tagtttgcca ctaccgaacc aataccgcgg acgaagttag
     2401 caaaggtggt tatcgaagac cacggaatga tgtgatacgc cccggtatcc tggcgcacgc
     2461 ccacggagcg aatcgacatc cgttccacag tgccggtcaa cggcccgata gtcaccaaat
     2521 ctccagtgtt catgccgttt tcaaactgaa taaatactcc ggtaataata tctttcacca
     2581 gcgtttgcga accaaacgag atagccagac ctaatgcccc ggcacctgcc agcaatggcg
     2641 cgatattgac gccgatttcc gacaacacaa tcatgatggt gatggtactg ataatcaccg
     2701 ccagcgcgtt acggaacagc gtcagcaggg ttcgcgtacg ggcgctgggt agcgggcggc
     2761 catgaatatc cgaagccagc cggttttcta tcaaactggc gagcaccgtc cagccaaccg
     2821 ccgagaagaa aagaatgagt gcgatacgga tcaggatatc tacggttttc tgccccgcgc
     2881 cgttttgcag ccagttccag aaatcgaaca atccccatgc gctcaacagc agcattaccg
     2941 ccacgcagac tgtcagaata cgcgctgttt tcagtgccgc cgaaagccag ccattcagcc
     3001 gtttttgcag ctccggataa tttcgctgag tatgtggcga gagggtgatg gttttcgcca
     3061 gccagcggga aaacataccg gaaacaaatg ctgcgatacc aataatcgcc aggctgcgca
     3121 ccgttgcacc catcataaat ttcaggctgt tgcccggatc gaacaacgaa aagaaaaaca
     3181 gcacgataaa ataggcgctt gccagccagt gccacaccag cgcaaaggcg cggataaaca
     3241 ggctgaaaaa ggccagcgaa tgctccgcaa agttgagcaa atgctgggtg atctcttttt
     3301 tattacgaaa aatcaggtac aacgcccaga cggtcatgca cagcataatg atgacgttcg
     3361 ccagcgcacc tatctgtaca ttcacctgat tagagataat cggcacagcc acaatcagac
     3421 catagccaat caggctgctt aaccagctca ggcgacgact ccagtaacgg gcggtctcgt
     3481 catgaatcgt gaacgggcgc agttcagcta cgtttgggca aaaaatcagg cgtagtacgg
     3541 ctttgaaaaa ttcaatcagg gcaaaggcgt tgagaaacaa actttgttgg aaagcgatag
     3601 tgcgactgcc cgcattcagg ttgtcgctta atacctggcc gacaaataat gtcagtgcca
     3661 gtaacagcag gtcgataata aacgccccga tgatcatcgc cggaagctgc aaccagttac
     3721 tgcgctcacg atttttttgc cgcgcccact ggcccatttt gcgatacagc ggcagtgcgc
     3781 acagacgaat cagccagtaa aaaccaaaca ctaataccgc taacattgaa aagtgggtca
     3841 gcgcattgct gaaggtttgt ggattaaacg gcttatgcgg ggagccggtg atattgcgat
     3901 aaagttgccc gaagcgggcg gaaagggctt caccataatg gcggctgact tcggtgactt
     3961 tttgcagcac ggtttgctct tcaaccagcg tcggcggcac gatcttcggt acgggttccg
     4021 caggaggcgt agcggcgacg gtgcgcaact ggtcgatcaa ctctttacgc gaggtgtcat
     4081 tatccagcac atccgccagt gcgccatagg ccgctttttt ttgttcgata tccggttccg
     4141 gggccggttc agtcgttgag tccgttgtcg ttgtggttgt aacgccgggt atggataccg
     4201 cgtgggcagg tgcgcccaac aggcagaaga ggatgaacag gatccaccgc atgactcctc
     4261 cagtgagaaa acagagcaaa aagataagta tagatgctgg gggagaggcg attttaaatg
     4321 agaggaatct ggtgtgcctc cctttcgggg gaaagggagg cagagaagtt aagagacgtg
     4381 ctgcaaaaat tcctgcaagc gctggctcgg cgggttcttg atcaacacct gcggatcgcc
     4441 atcttctgca atccggcctt tgtcgataaa gatcaggcgc gaagctactt tctcggcaaa
     4501 accgatttcg tgggtcacga tcaccatcgt catcccttct tcagccagat cctgcataac
     4561 cttcagcact tcatggcgca gttccgggtc aagagcggaa gtcggttcat caaacagcat
     4621 cattttcggc ttcaccgcca gcgcgcgggc aatcgccaca cgctgctgtt gaccaccaga
     4681 aagttcggaa gggtagtgat gtgcacgctc tgccagaccg actttcgcca gcagctcacg
     4741 cgccagtttt tccgcctctt ctttattcgc gccacgcacg cgtagcgggc caaacatgac
     4801 gttttccagc gccgtcagat gcgggaagag gtaaaactgc tggaacacca tacccgcttc
     4861 ctggcgaatc aggcgctcat caactttcgg atcgttaacc ttcaggccat cgacaatcag
     4921 atcgccggag gtgatttctt ccagtttgtt gatgcagcgc agcagggtcg atttaccgga
     4981 accggacggc ccgataatca ccacgacttc gccctgagca atgttcaaat cgatattgtg
     5041 cagcacctgg gttggaccaa aatgcttgga gacgttttta aattcaatca caggattttc
     5101 atccttcttt ccagacgacg cagaataaag ctcagcacca gggtaataat cagatagaac
     5161 accgccacgg cgctccagat ctcaagggcg cggaagttac cggcaataat ttcttgcccc
     5221 tgacgggtca gttccgccac gccgataaca ataaacagcg aagtgtcttt aatgctgatg
     5281 atccactggt tacccagcgg cggcagcata cgacgcagtg ccagcggtaa aatgacgtag
     5341 cgaatggttt cccaacgtga aagaccgagc gccagtcctg cttcacgaaa acctttgtga
     5401 atagacagca ccgcaccacg cgtgatttcc gcaatgtacg cgccggagtt gatcatgatg
     5461 gtgaccaccg ccgcagtaaa cgggtcgatg cgtaaatcgt taaacgccat cggcagggcg
     5521 aaatagataa acatcacctg gacgacgata ggtgtgccgc ggatcacttc aataaagacc
     5581 agcgcgacgt ggttggctat ccaaccaccg aaggtgcgtg caaaacctgc caacaatccg
     5641 atcaccagac cgccagccag accgaggacc gaaatccaca gggtcatttt ggcaccttca
     5701 atcagaagcg gaatggcagg ccagatggca ctccagtcaa actgcatatg ttgttcctgt
     5761 taccgcggtg taaataatca aattcagggt aattaatggc cccggagcgg gtttgcgccc
     5821 ttcaccctaa tcctctcccc ggcggggaga gggaactgcc agtgcgcttt acaggcgccg
     5881 cgttattatt tcggttcagt accgaaccat tttttgtaga tttcgttgta agttccgttc
     5941 tcgcgcaggg ttttcaacgc gccgttgact ttgtcacgca gctcgtcgct acctttcggg
     6001 aacgcaatac cgtattgctg tgcttccaga gagtcaccta ccgctttgaa ctgaccgtta
     6061 ccggcggttt tgatgaagta cagaatgttt ggcgtatcgt gcagaacggc gtctgcgcgg
     6121 ttggtgccca gttccatata ggcgttatcg atattcggga actgacgcag atctttagtt
     6181 ttgatgtttg ctttcgcgta atcaacggag ccagtgccgc tcttcacagc aaccactttc
     6241 ccgtcgagat ctttcacgct tttcacatcg ttattgttag ctttcaccat cactaacagg
     6301 ccgcttttgt agtagccgtc agagaaatcg atcgcttttt tacgctcgtc ggtgatggta
     6361 atgcccgcca gcgccagatc gacgtttttg gtttgcagtg ctggaatgat cccactgaaa
     6421 tccatcggct tcagttcata atccagcttc agttctttag cgatggcagc ccacagatca
     6481 acgtcaaagc ccacgtattt atcgccctgt ttaaattcaa acggaacgaa ggcagtatcc
     6541 gtcgcgacaa ctaatttttt atccgcggca tgagaagaaa ccgcaaaagc cagggtcagt
     6601 gcagccagtg aaacttttaa tacagacttc atagcatttc cttttttcta tccacgggac
     6661 gatcccctgc gtgacattca catatatgaa aaaatcgtgc cagttttgca actcattgat
     6721 ttttcgagat gcggcaaaaa cgtgatgcac gatttatagg gcaataccct gaagatgcac
     6781 cattctgggg caccaaactg gtgcgctaaa attgtgcact caacacagtg attatttagc
     6841 gtaaaacctg atgaaaaaac aatctttatg taacgattgt gtgatgatgt ggatacaaaa
     6901 aatttactta accagctggt gatagcagat ggatgcacta aataagtgcg ttgaggcggg
     6961 ctacaaaaca aaacggcctc ctgtcaggaa gccgctttta tcgtgtacta aagttctgca
     7021 ccatcagcga tggatttatt cgatgttaga ctcgataaac cacaggaatt tatccaggtc
     7081 gcgagacgcg gcggtcagga tatctgcggt gtcgtcatct ttcgcttcgc caatcgcttt
     7141 gcgtacgtca ttagcgacga ttgcgtaacg gtcagccagc tctttcaggt gatcctgaac
     7201 gttgtggatg tccagcgggt aacttttcag cggagttttg ctgttgataa cttgagtggt
     7261 ccccagagct acaccgccca gctgcactgc acgttctgcc atggtatcca gatgatcgat
     7321 cagtgcagtg cggaagccat ccagcatttc atgtacggca atgaagttag cgccgcgcat
     7381 gttccagtgc gcttgtttgg taatcaaaga aagatcaata aactggataa cctggcgatt
     7441 cagcaactct actgttgctt ttttctcgct gtcagagaca tcgttgcggg tataaagcag
     7501 attggtcgct tttgatttaa ctaatttagc ggtactcata atttcatatc ctcttgatgt
     7561 tatgtcccag taattaacga gactaagtat agcaccagct atgtgttccg ctattctggc
     7621 tgttcctatc acactaatag tggtaacaag cgtgaaaaaa caaaaccaat aaaaactcaa
     7681 tgagttagat atattaataa gaacaattct tatttatttg ctcgccagga attttcggaa
     7741 taaatatgct tttgcataat tcgatatata aagacggcgt agaggaaaag tagcgagaaa
     7801 atctgcatgg ttatgcataa ccatgcagaa atgtaaattt aatttatgtc taattctttt
     7861 attttgctct ctttgcgtac tgtcagcgta gaacccattg aagcggcgat gatagcgccg
     7921 agcgccagca gctgtatggg tgtcagtgtt tctccgagga aaatcatccc ggaaacggca
     7981 gccagcgcag gttccatgct cattagcgta ccaaatgtgc gtgttggcaa acgggtgagg
     8041 gcaatcattt ccagcgaata aggcagagcg gtcgagagaa tagcgacagc cagacccaat
     8101 ggaataaccg accagtgcca gagtgcttca ccagcctgaa gcgctccaat tggcacgaaa
     8161 attaacgctg caatcaacga accaattgcc accgtcgcag gaccatgttc cgctccagcg
     8221 cgttgcccac ttaaaatgta aatagcccaa caagccccgg cccccagtgc cagtgcacag
     8281 ccggttaaat cgacatggga aacgtcttgc cccagcggta gcaggaacca cagaccaaga
     8341 accgccagca caacccagac gaaatctacc gggcgacgag aagagaacag tgccaccgcc
     8401 agtggtccgg tgaactccag cgccaccgca atacccagcg gtaccgtctg aatagaaaga
     8461 taaaaaagat aattcattcc acccagcgaa acgccgtaaa acaacagcgg taatcgttgc
     8521 tctttggcaa agcgcagtcg ccatggctta aagaacgcga tgaggatcag cgtgcctaat
     8581 gccagacgca gcgcagtgac ccccggtgcg cccaccagag gaaaaagtga cttagctaac
     8641 gaggctccac cctgaataga cgccatggca acgagcaata tgactattgg taaccagacc
     8701 ggcattttac gtaatgaacc aggcatcctt tctcccacaa atatctaaac ctaagtaaag
     8761 cgtggagtgt actggatata cccaatgctg gttgagcatt tgttgaaaaa attttccccc
     8821 gttttgacta aaatgcgcca ggattgatgg aatcattagt ctggtgatta ggaataatct
     8881 ggatgaatga cagggaaaac atgcgtaata cttacgcagt tctctgaaaa agtgatttaa
     8941 atttagatgg atagcggtgt atggaaacgt tctgttacat gaaatggccc gttagacatc
     9001 ataaatcgcg aagagtttcc cattaatttt tgatatattt aaaacttagg acttatttga
     9061 atcacatttg aggtggttat gaaaaaaatt gcatgtcttt cagcactggc cgcagttctg
     9121 gctttcaccg caggtacttc cgtagctgcg acttctactg taactggcgg ttacgcacag
     9181 agcgacgctc agggccaaat gaacaaaatg ggcggtttca acctgaaata ccgctatgaa
     9241 gaagacaaca gcccgctggg tgtgatcggt tctttcactt acaccgagaa aagccgtact
     9301 gcaagctctg gtgactacaa caaaaaccag tactacggca tcactgctgg tccggcttac
     9361 cgcattaacg actgggcaag catctacggt gtagtgggtg tgggttatgg taaattccag
     9421 accactgaat acccgaccta caaacacgac accagcgact acggtttctc ctacggtgct
     9481 ggtctgcagt tcaacccgat ggaaaacgtt gctctggact tctcttacga gcagagccgt
     9541 attcgtagcg ttgacgtagg cacctggatt gccggtgttg gttaccgttt ctaatcactt
     9601 cggtgatata aaaatccgcc tctcggggcg gatttttttg ttttaaggtt tcgggtcgaa
     9661 attatcggtt ccgagatggt tgtaatccac cttctgtaac tggaagttgg tgatgtatat
     9721 cggcccggct ttcttctcag aaataaacgg gtatttgata tttatttcct tcgctttaat
     9781 tcccgtccac tgcgagaaaa agcctaagaa gtcattggca gagcggcggg ctttaatcac
     9841 ccgatgcgct ttatcgtcgc tggaaatgac cataaaaggc acctggaaat tttgctgata
     9901 cttatcatca tgagcaaggt attgcacgtc tttgccgcgc tctttaaagg ccagaccgtg
     9961 gtcagaaaag taaaccagcg agaagctgct gccgctgttg cgtaactgat cgtacagctt
    10021 gcgcagtaaa tcgtctgttt gcgtcatggt atagagatag cacgacgttt ctttcgattg
    10081 cacaaaggtt tcgtattttc cttgtgtccg gtcgcaggcc tgcggatgcg agcccatcag
    10141 atgtagaaca atcagctgtg gttgcgagtg ctcttgcgcc agcacttgag cggtcatatc
    10201 cagtaacgct tcgtctttcg tgtttttatc tgcttcaaaa ttaccttctt tcaggaagta
    10261 cacttcatct gctcgtttgg cgatgctggc gatggcggta tcgtattcgc cgatttgacc
    10321 ctggttggaa aaccaccagg tctggaagcc cgcgcgattt gccagggtga caaagttatc
    10381 ctgaaactgt ggtttgccat cgacaacgcg attgagcgtt aagccaagcg atttctgcgt
    10441 cgagccactg gcggcaatgt agtcagcaaa tatcaaaccg ttaacgctgc tggcaaacgg
    10501 ggtattgtcc cagtgaccgc caaaggcacc gagggcatcg cgacgcgcgc tttctccgat
    10561 caccaccaca taggtctgat acttcggctt aacggcagtg accgtccagg tatctttcat
    10621 tccggaaagt tttgccatcc gttcttgctc ttcaatcacc tcgttattat tgacgatgac
    10681 atccttagca aaacgaaata ccggatagcc agtatctttc agtttaaata cgccgcccca
    10741 ggccaggttt tgcacgggag caacaaaaaa tgtcgctaca ctgaatacca ggcacaggct
    10801 gtcgaaggta ttccagcgcg ctttttcact ctcttttttt cgcctgatcg ctattacgcc
    10861 gagcgcaaaa ataaatatgc cgaccaggta gctgtaccat ggaaaaatcg tcaggatttc
    10921 ggtcgactct tccatattgg tggagtgcaa tgccagcaat gtattgaagt taggcgcgcc
    10981 gtaggcctga gcaaaaggga aataacaagc cgccaccagc gagctgatac cgaccagaac
    11041 tttttgtacg cgaggcaatg ttcgccatag caaaagcaaa atagccgtaa acgcagcggt
    11101 gtagagcaaa ctgaaggggt aacctaagcc gaggttaatt aatagcgact gtaaaaagta
    11161 gaacgcagtc cacgggctaa atacccggct acgggtaaca agcgattctt tgagggttaa
    11221 attcatatgc cactatcgta acaaacgcca tgtgcttacc ctggcgtcaa gggtcaatac
    11281 ctgcaagagt gcgtgaagag gggaaagggg tccgcatccg cgagccgcaa tatacgcagg
    11341 gctgcaagaa gatagagcga gctgctgttc aggtcaacta ccgacgaaca aatcttttgc
    11401 gaaggggatt gcaaatccga caaaaagtgg ggtttttcag accaaaacgc gttgtgcctg
    11461 cataaaagca ggagtgaagc ggcgtaccgc gacgccgcta tttatcggaa ggtttatcct
    11521 gctgcggttt gttgttgacc atatcgcaca acatagagag cagcattaac cgtactttaa
    11581 agggagaatg actaaacacg cgcatacacc tcttgaactc attcataaga cctccttact
    11641 tgctaatccc atcgatcctt gagggatgat tgcattatat acagatatag cacaggctat
    11701 attatatagc tattgctaaa acgttaattt tttgtaccct cgcaactctg gtttacaatg
    11761 tgcgcacgaa atgagaacgc tatgtatgcg tcaccataat gaggaagcac aatgagtcgt
    11821 cgcgcaggta cgccaacagc aaaaaaagtg acgcagttag tgaacgtgga agagcacgtt
    11881 gaagggttcc gccaggtcag agaggcgcat cggcgcgagc tgattgatga ttacgttgag
    11941 ctgatttctg acttgatcag ggaagtgggg gaagctcgtc aggtggacat ggctgctcgt
    12001 ctgggggttt cgcaaccgac ggtggctaaa atgcttaagc gactggcaac catggggctg
    12061 attgaaatga tcccctggcg tggcgtgttt ttaacggcag aaggagagaa gctggcgcag
    12121 gaaagccgcg agcgacatca gatagtcgaa aatttcttgt tggtgttggg cgtcagtccg
    12181 gaaatcgccc gtcgcgacgc ggaaggtatg gagcaccatg ttagtgaaga gacgctggat
    12241 gcctttcgtt tgtttaccca gaaacacggt gccaaatgag cctgcctttt ttacgcacgc
    12301 tgcaaggcga tcgttttttt cagttattaa ttcttgttgg catcggatta agttttttcg
    12361 ttccctttgc accgaaatcc tggcctgctg ctatcgactg gcacaccatc atcaccttaa
    12421 gcggcctgat gctgctgacc aaaggtgtcg agttaagcgg ttattttgat gtgctggggc
    12481 gcaaaatggt gcgccgtttt gctacggagc gtcggctggc gatgtttatg gtactggcgg
    12541 cagcgctgct ttctaccttt ctgaccaacg atgtcgcact gtttatagtt gttccgctga
    12601 ctatcacgct aaaaagactg tgtgagattc cggttaatcg gctgattatt tttgaggcgc
    12661 tggcagtcaa cgctggttcg ctactgacgc caattggcaa cccgcaaaat attcttatct
    12721 ggggacgttc tggtctttcg tttgccggat ttattgccca aatggcaccg ctggctggcg
    12781 caatgatgct gacactcctg ctgttgtgct ggtgttgttt ccctggaaag gcgctgcaat
    12841 accatacggg ggtgcaaacg ccggagtgga aaccgcggct ggtgtggagt tgtctggggc
    12901 tgtatatcgt ctttctgacg gcgctggagt taaaacaaga gctgtgggga ctggtgattg
    12961 tggcggcggg ctttgcgctg ctggcgcgtc gtgtggtgct cagtgtggac tggacgctgc
    13021 tgctggtgtt tatggcgatg tttatcgacg tccatttact aacccaactg ccagttttgc
    13081 agggggtgtt aagtaacgtg agtcatttat ccgaacccgg attatggtta acggcgatcg
    13141 gtttatcgca ggtgatcagt aacgtgccga gtaccatttt gctgctcaat tatgtgccac
    13201 cgtctttatt actggcatgg gcggtaaacg tggggggctt tggtttatta cccggatcgc
    13261 tggcaaattt gattgcgtta cggatggcga acgatcgccg tatctggtgg cgttttcatc
    13321 tttattcgat accgatgctg ttgtgggcgg cgctggtggg atatgttttg ttggttatga
    13381 tcccggcggg ataaggtctg tcaaattaaa aaggcggatt atgagtccgc ctttgcttta
    13441 cgttttgaag attaattcag acgaaccggc atcccggaac gatttttgat cgcttcatcc
    13501 agaacaacct ggtcaacatc tggctgaccg gtcactgtct gcacgctctt cgtcagggta
    13561 attggcacaa tttcctgacc ttcaaactgg gcttcggttg tagacagcgg gttatggact
    13621 tcaatataac ggctgccgtc tggctcggtg gtcgctttta ccggctcatc aataaactgt
    13681 acgcgggtac cgaccggtac tttctcgaac aggaatttga tgtcttcgtt acgcagacgc
    13741 acacaaccat gacttacacg caggccgata ccgaagttgg cgttggtgcc atggatagcg
    13801 tacaggcgac cgatgtagag tgcatacagc cccatcgggt tatccggacc agccggaacg
    13861 accgccggaa gcggttcgcc cgcagcgcgg tactctgcgt gcattttagc ggtcggcgtc
    13921 caggtcgggc ctgctttttt acgctcaact ttggtggtcc agttgatagg cgtgtctttg
    13981 cctaactgac caatgccgat cggcagcacg ataacggtgt tggtcccttt cggatagtaa
    14041 tacagacgca tctctgcact gttaatgacg atgccttcat gaacggtatc cggcaggatc
    14101 agctgctgcg gaatgttcag tacagtaccg cctttcggca ggaaggtatc cacacccggg
    14161 ttcgcttcca tcatattgga aagtcccatc tggtactcgg cggcaaaata ctccagcggc
    14221 tgagtgttac cttcaggaat ggtgatcacc tgattctgac caaccaggcg actcccgtcg
    14281 gttggcagag gataagttac cgcagaggcg gtactgcaaa agccgacaac agcgaaggcc
    14341 gctgcgaata atgttttcaa tttcatattc atgttaagcg agattttgtg cctggcaggc
    14401 cattgggttg agaatattag agtattggaa gcgcattata agttcattcc agctcacagt
    14461 gaaatcagat gtgtacgaaa tcacattttt tgcctttggc ttgagtgtag accttaagcg
    14521 aggagcagga tcttctttca gacttatggc ataatgcgcg gtttgtcata tctcttttca
    14581 ggatacgcct gtgttagttt ccagtaacgt caccatgcag ttcggcagta agccgttgtt
    14641 tgaaaacatt tccgtcaaat tcggcggcgg caaccgttac ggcctgattg gcgcgaacgg
    14701 tagtggtaaa tccaccttta tgaagatcct cggcggcgac ttagagccga cgttgggtaa
    14761 cgtttccctc gatcccaatg agcgcattgg taagctgcgt caggatcagt ttgcctttga
    14821 agagttcact gtgctggata cggtgatcat ggggcataaa gagttgtggg aagtgaagca
    14881 ggaacgtgac cgcatctatg ctttgccgga aatgagtgaa gaagacggct ataaagtggc
    14941 cgatctggaa gtgaaatacg gcgaaatgga cggttactct gcggaagctc gcgctggtga
    15001 actgttgctt ggcgtgggaa ttccagtgga acagcactac ggcccgatga gtgaagttgc
    15061 tcctggctgg aagctgcgtg tgctgctggc gcaggcgctg tttgctgatc cggacattct
    15121 cctgctcgac gaaccgacca acaacctcga catcgacacc attcgctggc tggaacaggt
    15181 gctgaacgag cgtgacagca ccatgatcat catctcgcac gaccgtcact tccttaacat
    15241 ggtttgtacc cacatggcgg atctggatta cggcgagctg cgcgtttatc cgggcaacta
    15301 cgatgagtac atgacggcgg cgactcaggc gcgtgaacgt ctgctggctg ataacgccaa
    15361 gaagaaagcg cagattgctg agttgcaatc tttcgttagc cgctttagcg ccaacgcctc
    15421 gaaatctcgc caggcaactt cgcgcgcgcg ccagattgat aaaatcaaac tggaagaggt
    15481 gaaagcctcc agccgtcaga acccgttcat ccgttttgaa caggataaga aactgttccg
    15541 taacgcgctg gaagtggaag gtctgactaa agggtttgat aacggtccac tgtttaaaaa
    15601 tctcaacctg ctgctggaag tgggtgaaaa actggctgta ctgggtacca acggcgtcgg
    15661 taaatcaacg ctgctgaaaa cgctggtggg cgatctgcaa ccggacagcg gcaccgtaaa
    15721 atggtctgag aacgcgcgca ttggttacta cgcccaggac catgaatatg agttcgaaaa
    15781 tgatctgacc gtgttcgaat ggatgagcca gtggaagcag gaaggcgatg acgagcaggc
    15841 ggtacgcagc attctcggtc gtttactgtt cagccaggac gacatcaaaa agccagcgaa
    15901 agtgctttcc ggtggggaga aagggcggat gctgtttggt aagttaatga tgcagaagcc
    15961 gaacattctg atcatggacg aaccgaccaa ccacctggat atggaatcca tcgagtcgct
    16021 gaacatggcg ctggaactgt atcagggcac gctgatcttt gtttctcacg accgtgagtt
    16081 tgtaagctcc ctggcgaccc gtattctgga aatcaccccg gaacgcgtga tcgactttag
    16141 cggtaattac gaagattacc tgcgtagtaa agggatcgag taagttatta tcagtagccc
    16201 tgatatggtc agggctactg aaactgatga caaacgcaag attgcctggt gcgctacgct
    16261 tatcaggcct ggtagtgtat cgcaatatat tgaattttca tgattttgta tgccaaataa
    16321 ggcggttacg ccgtatccgg cataatgaca tgcactttct caataaactg aattggcgat
    16381 aatcaaagat taaacggcgg tttattttcc agcaccgcct gaatcacatt cagcgcgcct
    16441 tcatgattat tatcatcggt agcgtaacgg gcgatttgtt taatgttttc cgcagcattg
    16501 ctcatcgcaa aggaataacg cgccattttc agcatctccg catcgttacc gctgtcgcca
    16561 atcgctacca cattttgcgg tgacagatcc cagcgtttca gtaaccgcga aataccgttt
    16621 gctttatgta gaccgggaat aatcaagtcg ataaagccaa aaccactggt gacgggtttc
    16681 ataatgccat ccagcgctat gtgcagtttg tcgatcacta acgggatttg ttcatccggc
    16741 aggttgagcg aaaacttgaa cagtacgtcg tcaatctcct gataatcttt tacaggtttc
    16801 aggcgatggt agtgttttgc catcagtgcg acgaatgctt cgggggcatt ttcgctgaca
    16861 tatgcacttt gcagaccgca ggcgacaaaa ttgagttgct tatcttttag caactcgcca
    16921 ataacaatcc gcgattcatg tcgggtcagt tcgccgtgga acaactgctt gccatgttcg
    16981 taaaccagtg cgccgttttc cgcgacaaaa gagatctcat cctttagctc aggaaagaac
    17041 gaaataagct ggtaatactg attaccgctg gcgacaacga actcaatgcc gcgcttttta
    17101 agttcctgat actgcgccat aaaacgtggt tggttgtacg ttttggcgtc gttaagaaaa
    17161 gtaccgtcca tgtctgtgac gataactttt acgctcatag gtgtgctcct ggctcgaaaa
    17221 tgaaaccgta acagtgtaat aacaatgtga cgcagagcac aaattatatt tcgaatgaaa
    17281 gtgagagcgg aattgatgat gtgaatgatt tgacccggcg acgacgccgc cgggctgagg
    17341 agattacagc atatgttcag tacgggcgat gatatcgtcc tgagcgtctg gagagagcgc
    17401 ggtgaagaag gcggaatagc ccgcgacacg cactaccaga tcgcggtact gatccggatg
    17461 ttttttcgct tccagcagcg tttcgcggga aacgatgttg tactgaatat gccagccttt
    17521 atgcacctcg aagaaggtgc gcagcaggat catcagtttc tgcttgtcag attcgttctc
    17581 cagcgttgct ggattcagtt tctggttgag cagcacgccg ccgagaatcg cggccgtagg
    17641 cagtttaccc accgagccaa tgaccgcagt agggccgaga tggtcagtac cggaggccgg
    17701 actagcgcct tctgccagcg gggtatgtgc tttacgtccg tccggcgttg ccatcgtctg
    17761 cgcgccaaac ggtacgttag cggagataga cgatgtgccc gcataatagt tgccgccaat
    17821 cggaccacga ccgtagcgcg ggttatggta ctgcttcagt tcgtcgatat aggtctgata
    17881 agcgcgagcc agcagcgtat cgacagtatc atcgtcgttg ccgtacttcg gcgcaccgtt
    17941 aatcagccgc tgacgcagct gctcgtgagt cagaccgtcg aagtcatccg ccagtgcggc
    18001 agccagctgt tgctgaccaa tcgcgccctg ttcaaatacc agtttcttca ctgccgccag
    18061 gctgttgccg aggttagcaa tgccgacctg caggccagaa acccagtcat atttcgcgcc
    18121 gccttgcttg atacttttcg ctcgctcaat acagtcatcg accagcgccg agcagagaat
    18181 atcgtgtacg ttctcttcca gcatggtatc gacgacatat tcgatttcga ttgatttacg
    18241 cgtgtagtaa cggatttgcg tatcccatgc gtccattact tcatcgaagt tgttgaagtt
    18301 acccgctgac aacgcttttg cttgtggcag gaacactttg ccgctggtgg catcacgacc
    18361 accttccagc gccgccagca tcacgcgggc gaagttgata aagctcatgc cggtacagcg
    18421 atagccccat ttgccaccga cggcggtttc gatacaacca attgctgcgt agtcgtaagc
    18481 gtcctgcggt tcaataccga gtttaataaa ttccgggatc acgatttcgt cgttgttgaa
    18541 cgccggcatc ccgaagccgc agcggatcac ctgaacacag gcgtcgagga aatcgttact
    18601 catacccgca tggtaacgca cgctgaggtt aggctgagtc gaacgcaggc gaccgcaaga
    18661 ttcgaggatc gcataagaga gtggatttac cgcgtccatc ggttgaccat caaccagatt
    18721 ttgcccgcca atggtgacgt tctgatacag cggacttccc gcagaggctt ttgagtgtga
    18781 gccggagcgg attttgttca cttccagcag tttcagccag cagctatgca gcatctcgat
    18841 ggcgtgttcg cgatccagtg tctggttcag ttcaacgtca cggcgatagt atgggtagag
    18901 atactggtcc atacgaccaa acgataccga gtgaccgtta gattcgatct gcaaaatcaa
    18961 ctggatgaag tagcacagtt gcagcgcctg ccagaaagtc tgcggcggct ggtgggcgat
    19021 aagatcgcag ttttctgcta tcgtcagcag ttcatcgcga cggctttcgc gggtttcggt
    19081 cgcggccatt tcacgcgcca gggcagcgaa acgttcaatg tgttcactga ctgccaccag
    19141 cacgatatca atcgctttca ggaattgctc gccgtgcaga tcttccagaa ccgtcagatt
    19201 gatgtgcgag cgacgttccg ccactttctc acgcagacca tcaagacctt tttccagcag
    19261 tagcgggaaa ttgaccgcca ggtgcgcatc gccggaggtc atattgccct ccgctttaat
    19321 gattccggtc gccagcagac ctttttgctc atcggtaaac atgccgtagc agcgatcctg
    19381 tacggtctga ccgcgccacc acgggcacac ttcatgcaga acgcgtttgt tctcttcgct
    19441 caccgcaaaa ccagcaccgg gacgatctgc cagatcatca atctcttttt cgatccacga
    19501 gacggtatat tccgggaaga tcggcgcggc gcgaacttcg cttgcctggt tgccaatgat
    19561 caactcatcg tgcttgatcc agatggtgcg attcgccagg tgatgcgcca gtgccagcgc
    19621 gcgacgtacc gggattggct tatcgagatg ttgttgatac atctcggtat agtgttgtgc
    19681 tcgttcggta cagaccggcg gtttcacaat atgcaccagc gcatttttgt gcgctttaat
    19741 gcggtcgctg agcgtatcca gttttagtgt tgtcatgatt gttatcctcg taaggtcgcg
    19801 gttaaccctt tctggcaggc atactgctgg gcaaagtcga gcagttctgg cgcatcaagc
    19861 ggtttttccg gggcgtcata gggcagatta agtaagtgat atttgttgat gcccagtgtg
    19921 tggtagggca gaaaatgaat ttcgctaacg tgcagctcgt cggcggcaaa atcggtaatg
    19981 gctttcacag aagtttcgtc ggcattaaag ccctgaatca gtggcacgcg gataatgatt
    20041 tttttgccgg ctgcggcgag ttttttcagg ttatccagca ccctggcggc gttaccgtcg
    20101 gtccactgtt taaacggcgc gtcggcaacg tgttttaaat cggcaagaaa cagatcgata
    20161 tagggcagag aaggggcgat atatttccac ggcacatgca gacaggtttc taccgcagta
    20221 tgaatgccgg cctcgtggct ggcttgcagc agcgccgtcg ccatttccgg ctgcataaac
    20281 ggttcaccgc cagaaagcgt taagccgccg ccgctgcgat cgtaaaacgg tttatcgcgc
    20341 aaaacggttg ccatgatctc ctcaacgctt ttcacttcac cacacacggt taacgcctgt
    20401 gtcgggcagc agtcggttag cgccgtaaaa tgctccgggg ttaacttttc ccgatgaata
    20461 agcaaaccat tcaacgcgcg ctcaatcact tccggtgcgg tcttagcgca cagcttacag
    20521 ccttccagac agagtcgtgg gtcatacagc agatcctgcg tgcgggcgcg gctttccggg
    20581 ttctgacacc agctgcagcc cagcgaacag cctttaagaa atacgaccgt gcggataccg
    20641 gggccatcat gggtcgagta gcgttgaata ttgaaaatca tagttgcctc tctgtttcgt
    20701 tcaaacatta aaatactttc gaatgaaagt tagattgatg tgcgtcaact gttcagagag
    20761 ttttcccgtg atagtctaca ttcagacaaa aagtacattt tgaggatggt tatggaactg
    20821 tatctggata cttcagacgt tgttgcggtg acggcgctgt cacgtatttt tccgctggcg
    20881 ggtgtgacca ctaacccaag cattatcgca gcgggtaaaa aaccgctgga ggttgtgctt
    20941 ccgcaacttc acgaggcgat gggcggtcag gggcgtctgt ttgcccaggt aatggctacc
    21001 actgctgaag ggatggttaa tgacgcacgt aaattgcgtt ctattattgc ggatatcgtg
    21061 gtgaaagttc cggtgaccgc tgaggggctg gcagctatta agatgttaaa agcggaaggg
    21121 attccgacgc tgggaaccgc ggtatatggc gcagcacaag ggctactgtc ggcgctggca
    21181 ggtgcggaat atgttgcgcc ttacgttaat cgtattgatg ctcagggcgg tagcggtatt
    21241 cagactgtga ccgacttaca ccagttattg aaaatgcatg cgccgcaggc gaaagtgtta
    21301 gccgcgagtt tcaaaacccc gcgtcaggcg ctggactgct tactggcagg atgtgaatca
    21361 attactctgc cgctggatgt ggcacaacag atgattagct atccggcggt tgatgccgct
    21421 gtggcgaagt ttgaacagga ctggctggga gcgtttggca gaacgtcgat ttaaccggac
    21481 gttctgcatc ctcataaatt gctgatgacg tggcggagtg ccgcgtctta tctggcctgg
    21541 aggtggcagt tactgcccgc acacctcaca ccccggatta cgcatcagtt tcatttcgcg
    21601 aaactgacag gtcatcgcat cgtacatcac gattttcccg ctggctggtt tgccataacc
    21661 tgccagcagt ttgattgctt ccattgcctg caacgaacca attacgccga ttaacggtgc
    21721 catcacgcct gcttctacgc aggttaacgc gttctcgcca aacaaacggc taaggcagcg
    21781 atagcacggt tcgccgtcct gataagtgaa gaccgtgatt tgcccttcca tacgaattgc
    21841 cgcgccggaa accagcggca acttcgcggc aaaacagcct gcgttcagtt gattacgtac
    21901 tgcaacgtta tccgtacagt cgagcaccag atcgtgtttt gcaatcatcg cagcaagttc
    21961 agcgtcatca agcagcgcat tgactggcgt aatcgcgata tgtgggttga tccgcgtcag
    22021 ggcgtcacgg gcggattcca cctttggttg cccgaccgtg gcatcactgt gcagcgtctg
    22081 gcgttgcaga ttggagagcg aaaccgtgtc gaagtcgagc agcgtcaggt tgccgacacc
    22141 ggcgcttgcc agatactgcg aagctgcgca gccgagtcca ccaaggccga cgaccagcac
    22201 acgagaatct tttagcgcct cctgaccgtc aaaatcaaaa ccgcgcagaa tgatttgccg
    22261 gttgtagcgc agcatctcct gatcgctgag ttctgccatt acaggcctcc gaacaacgcg
    22321 ttaaacggtt cgacttccac ccattcgccc acgtcaacat tgccgcgctc gcgttccagc
    22381 acgataaagc agttgccgag gctaaaggag ctaaatatat gtgaaccctg atgtccggtg
    22441 gtcgtcactt ccagttcgcc atcggcgttg cgttgcagca cgccgcgctg gaaatcaagg
    22501 cgtcctggcg tttttttcag gcgggacgct gtgcgtacac gctggcgcgc gggcaggcca
    22561 ctggcggtat tgccgcttag ttttgccagc aaaggctgta ccagttgata gaaggtcagc
    22621 gtcgctgaaa ccgggttgcc cggcaggccg cagaaccaac tattagaaag tttaccgaat
    22681 gcgaacggtt tacccggttt aatcgccagt ttccagaagg cgatctcccc cagctcttca
    22741 agaatcgttt tggtgtaatc cgcttcaccc actgaaacac cgccggaact gatcaccaca
    22801 tccgcctggc tgtcggcttc aataaatgcg gcgtgcaggg catggggatc gtcgcggata
    22861 atccctaagt tgattacctc gcatcccaac tgttctaaca tcaggtgtac ggcgagacgg
    22921 ttggtatcgt agatttggcc gtcgcccagc ggctgaccgg gcaactggag ttcatcaccg
    22981 gtagaaaaaa gcgctacacg cactttacga atcaccggaa cttcggcaat ccctagcgaa
    23041 gcaatcactg gcagttcagc ggtagtcagg cgagtccccg ccgggaaaac aaccgcacct
    23101 gcagagatat cttcaccgcg acggcgaata ttttgcccgc tacgcgcttc agcagtaaaa
    23161 cgcacgccat tgtccgtttg ttcagtctgc tcctgcatca ccaccgcttc gcagccttcc
    23221 ggcaccggcg caccggtcat aatacgaatg caggtacccg caggccattc accatggtac
    23281 ggctgaccgg caaaggattt accggcaacg ggcagcggtt gcccggaggc aatatcggct
    23341 aaacgcaccg cgtagccgtc catagcggag ttatcaaacc ccgggacatc cagtggcgaa
    23401 acgacatcgc tcgccagaat acggccaaaa cactgaacca gtggcagcgt ttcctgggcg
    23461 gtcagtgggg taacgcgaga aagcatctca ttaagcgcgg tgtcgagcga catcaatccg
    23521 gtggtaaatt ccatgaaaac actcctgcgg aggcaaaatc gaatttgcct attatgtcag
    23581 aaaaacgcca cagactgtat gccacctcgg gcgtagcgct ggctcctgcc tttacatgcc
    23641 atatccatct ttctatattc aaaaattgaa tgagtcattc ataaaaattc tgatatttat
    23701 agcaaaagtg gcgagccacc cttaatggac gaatactatg ggcaaagcag tcattgcaat
    23761 tcatggtggc gcaggtgcaa ttagccgcgc gcagatgagt ctgcaacagg aattacgcta
    23821 catcgaggcg ttgtctgcca ttgttgaaac cgggcagaaa atgctggtag cgggcgaaag
    23881 tgcgctggat gtggtgacgg aagcggtgcg tctgctggaa gagtgtccac tgtttaacgc
    23941 cggaattggc gctgtcttta cgcgtgatga aacccatgaa ctggacgcct gtgtgatgga
    24001 tggtaacacc ctgaaagccg gtgcggtggc gggcgttagc catctgcgta atccggttct
    24061 tgccgcccga ctggtgatgg agcaaagccc gcatgtgatg atgattggcg aaggggcaga
    24121 aaattttgcg tttgctcatg gcatggagcg cgtctcgccg gagattttct ccacaccttt
    24181 gcgttatgaa caactaatgg cagcgcgcga ggaaggggca accgtcctcg accatagcgg
    24241 tgcgccactg gatgaaaaac aaaaaatggg caccgtaggg gccgtggcgt tagatttaga
    24301 cggcaatctg gcggcagcca cgtccacggg cggaatgacc aataaattac ccggacgagt
    24361 tggcgatagt cccttagtgg gggccggatg ctatgccaat aacgccagtg tggcggtttc
    24421 ctgtaccggc acgggcgaag tcttcatccg cgcgctggcg gcatatgata tcgccgcgtt
    24481 aatggattac ggcggattaa gtctcgcgga agcctgcgag cgggtagtaa tggaaaaact
    24541 ccctgcgctt ggcggtagcg gtggcttaat cgctatcgac catgaaggga atgtcgcgct
    24601 accgtttaac accgaaggaa tgtatcgcgc ctggggctac gcaggcgata cgccaaccac
    24661 cggtatctac cgtgaaagag gggacaccgt tgccacacag tgatgaactt gatgccggtg
    24721 atgtgctggc ggttgaaaat ctgaatattg cctttatgca ggagcagcag aaaatagctg
    24781 cggtccgcaa tctctctttt agcctgcaac gtggtgagac gctggcaatt gttggcgaat
    24841 ccggctccgg taagtcagtg actgcgctgg cattgatgcg cctgttggaa caggcgggcg
    24901 gtttagtgca gtgcgataaa atgctgttgc agcggcgcag tcgcgaagta attgaactta
    24961 gcgagcagag cgctgcacaa atgcgccatg tgcgcggtgc ggatatggcg atgatatttc
    25021 aggagccgat gacatcgctg aacccggtat ttactgtggg tgaacagatt gctgaatcta
    25081 ttcgtctgca tcagaacgcc agtcgtgaag aagcgatggt cgaggcgaag cggatgctgg
    25141 atcaggtacg cattccggag gcacaaacca ttctttcacg ttatccgcat caactctctg
    25201 gcgggatgcg ccagcgagtg atgattgcga tggcgctgtc atgccgcccg gcggtgctga
    25261 ttgccgatga gccaaccacc gcgctggatg tcactattca ggcgcagatc ctgcaattaa
    25321 tcaaagtatt gcaaaaagag atgtcgatgg gcgttatctt tatcactcac gatatgggcg
    25381 tggtggcaga gattgccgat cgggtactgg tgatgtatca gggcgaggcg gtggaaacgg
    25441 gtagcgtcga acagattttt catgcaccgc aacatcctta tacccgtgcg ctgttagctg
    25501 ctgttccgca acttggtgcg atgaaagggt tagattatcc ccgacgtttc ccattgatat
    25561 cgcttgaaca tccagcgaaa caggagccac ccatcgagca gaaaacggtg gtggatggcg
    25621 aacctgtttt acgggtgcgt aatctggtca cccgtttccc tttgcgcagc ggtttgttga
    25681 atcgcgtaac gcgggaagtg catgccgttg agaaagtcag ttttgatctc tggcctggtg
    25741 aaacgctatc gctggtggga gagtctggca gcggtaaatc caccaccggg cgggcgttgc
    25801 tgcgcctggt cgaatcgcaa ggcggcgaaa ttatctttaa cggtcagcga atcgatacct
    25861 tgtcacccgg caaacttcag gcattgcgcc gcgatattca gtttattttt caggaccctt
    25921 acgcttcgct ggacccgcgt cagaccatcg gtgattcgat tctcgaaccg ctgcgcgtac
    25981 acggtttatt gccaggtaaa gaagcggctg cacgcgttgc gtggttgctg gagcgcgtgg
    26041 gcctgttacc ggaacatgcc tggcgttacc ctcatgagtt ttccggcggt cagcgccagc
    26101 gcatctgcat tgctcgcgcg ttggcattga atccaaaagt gatcattgcc gacgaagccg
    26161 tctcggcgct ggatgtttct attcgcgggc agattatcaa cttgttgctc gatctccagc
    26221 gtgatttcgg cattgcgtat ctgtttatct cccacgatat ggccgtggtt gagcggatta
    26281 gtcatcgtgt ggcggtgatg tatctcgggc aaattgttga aattggtcca cggcgcgcgg
    26341 tctttgaaaa tccgcagcat ccttatacgc gtaaattact ggcggcagtt ccggtcgctg
    26401 aaccgtcccg acaacgaccg cagcgtgtac tgctgtcgga cgatcttccc agcaatattc
    26461 atctgcgtgg cgaagaagtt gcagccgtct cgttgcaatg cgtcgggccg gggcattacg
    26521 tcgcacaacc acaatcagaa tacgcattca tgcgtagata acattcaggc ggagaataaa
    26581 atggcaagag ctgtacaccg tagtgggtta gtggcgctgg gcattgcgac agcgttgatg
    26641 gcatcttgtg cattcgctgc caaaaatgtg gtggtggcag taggatcgaa cttcaccacg
    26701 ctcgatccgt atgacgcaaa tgacacgtta tctcaggccg tagcgaaatc gttttaccag
    26761 gggctgttcg gtctggataa agagatgaaa ctgaaaaacg tgctggcgga gagttatacc
    26821 gtttccgatg acggccttac ttacaccgtg aaattgcggg aaggcattaa attccaggat
    26881 ggcaccgatt tcaacgcagc ggcggtgaaa gcgaatctgg accgggccag cgatccggcg
    26941 aatcatctta aacgctataa cctgtataag aatattgcca aaacggaagc gatcgatccg
    27001 acaacggtaa agattactct caaacagcca ttctcagcgt ttattaatat tcttgcccat
    27061 ccggcgaccg cgatgatttc accggcagcg ctggaaaaat atggcaagga gattggtttt
    27121 catccggtgg gaaccggacc gtatgaactg gatacctgga atcagaccga ttttgtgaag
    27181 gtgaaaaaat tcgtgggtta ctggcagcca ggattgccca aactggacag cataacctgg
    27241 cgtccggtgg cggataacaa cacccgcgcg gcaatgctgc aaaccggtga agcgcagttt
    27301 gctttcccca ttccctacga gcaggccgca ctgctggaga aaaacaaaaa tatcgagttg
    27361 atggccagtc cgtcaattat gcagcgttat atcagtatga acgtgacgca aaagccgttc
    27421 gataacccga aggtccgtga ggcgctgaat tacgccatta accgcccggc gctggtgaaa
    27481 gtggccttcg cgggctatgc aacgccagct actggtgtgg taccgccgag tatcgcctac
    27541 gcgcaaagtt ataaaccgtg gccttacgat ccagtgaaag cgcgcgaatt actgaaaaag
    27601 gcgggatatc ccaacggttt cagtaccacg ctgtggtcgt cacataacca cagcaccgcg
    27661 cagaaagtgc tgcaatttac ccagcagcag ttagcgcagg tcgggattaa agcccaggtg
    27721 actgcgatgg atgccggaca gcgggcggca gaagttgaag gtaaagggca aaaagagagc
    27781 ggcgtgcgga tgttctacac tggctggtcg gcttcaaccg gcgaagcaga ctgggctcta
    27841 tcgccgctgt ttgcctcgca gaactggcca ccgacgctgt ttaataccgc gttttacagc
    27901 aataaacagg tggatgactt cctggctcag gcactgaaaa ctaatgatcc ggcggaaaag
    27961 acccgcttgt ataaggcggc gcaggatatc atctggcaag agtcgccgtg gatcccgctg
    28021 gtggtagaaa aactggtgtc ggcacacagt aaaaacctga ccggtttttg gatcatgcca
    28081 gacaccggct tcagctttga agatgcggat ttgcaataag caacgcaggg agtggaatgc
    28141 ttaattacgt tatcaaacgc ttactggggt tgattccgac gctgtttatc gtctcggtgc
    28201 tggtgttttt atttgtccac atgctgcccg gcgatccggc gcgattaatt gccgggcccg
    28261 aagctgatgc gcaggttata gaactggtgc gtcagcagct ggggttggat cagccgctat
    28321 atcaccagtt ctggcactat atcagcaatg ctgtgcaggg ggattttggc ctgtcgatgg
    28381 tgtcgcgtcg tccggttgcc gatgagattg ccagccgctt tatgccaacg ctgtggctga
    28441 ccataaccag tatggtctgg gcggtgatat ttggtatggc ggcgggaatt atcgccgccg
    28501 tctggcgtaa ccgttggccg gatcgattga gtatgactat tgcggtgtcg gggatttcgt
    28561 ttccggcatt tgctctgggg atgcttttaa ttcaggtatt ctccgttgaa ctgggctggc
    28621 tgcctaccgt gggagcagac agttggcagc actacatttt accctccctg acgctcggcg
    28681 cggcagtggc cgccgtgatg gcgcgcttta cccgcgcgtc gtttgtcgac gttttaagcg
    28741 aagattatat gcgtaccgcg agggcgaaag gggtgagcga aacctgggtt gtcctcaaac
    28801 acgggctacg caacgcgatg atcccggtag tgaccatgat gggcttacag tttggatttt
    28861 tactcggtgg ttccatcgtg gtggagaaag ttttcaactg gcccggactt ggacgcttac
    28921 tcgttgactc cgttgaaatg cgcgattacc cggtgattca ggcggaaatt ctgcttttct
    28981 cgctggaatt tattcttatc aacttagtgg tggatgtgct ttacgccgcc attaacccgg
    29041 ctatcaggta caagtaagga tgcgactatt taactggcga cgtcaggcgg tgttaaacgc
    29101 catgccactg gtcaaacctg accaggtacg tacaccgtgg catgaattct ggcgacgatt
    29161 tcgccgtcag catatggcga tgaccgccgc attgttcgtt attttattga ttgtggtggc
    29221 catttttgca cgctggatcg ctccctatga cgccgaaaat tattttgatt atgacaatct
    29281 gaataacgga ccttctttgc agcactggtt tggtgtcgat tcactggggc gcgacatttt
    29341 cagccgtgtc ctggttggtg cgcaaatatc gctggcggcg ggcgtgtttg ccgtgtttat
    29401 cggtgcggcg atcgggacgt tgctgggctt gctggctgga tattatgaag gctggtggga
    29461 tcggctgatc atgcgcattt gcgatgtgtt gtttgccttc ccgggtattt tactggcgat
    29521 cgctgttgtt gcggtgttgg gaagcggcat tgctaacgtg attatcgcag tcgccatttt
    29581 ttccatcccc gcgtttgccc ggctggtgcg cggcaacacg ctggtgttga aacagcaaac
    29641 ctttatcgag tcagcacgca gtattggtgc cagcgatatg accattttgt tgcgtcatat
    29701 cttgcctggg accgtctctt ctatcgtggt gtttttcacc atgcgcattg gcgcctcgat
    29761 tatctctgcc gccagcctgt catttctcgg cctcggtgcg cagccgccga caccagagtg
    29821 gggtgcaatg ctcaatgagg cacgagcgga tatggttatc gcgccgcatg tcgctgtttt
    29881 tccggccctg gctatttttc tgaccgtact ggcgttcaat ttgttgggcg atggtttacg
    29941 cgatgcgctg gatccgaaaa ttaaaggata gttacgtttg aatattgctt gaaagggtaa
    30001 tcacctcaca ggaaattatt gccctaagca agtgttgtaa ctttctgctg attttgtaga
    30061 atcgggtaat ttggttaaaa agccgcagca agggactatt ttgcagcggc acagcgttca
    30121 gacagttatt ttgttaaatg tattaacatg ctgagtttat acgaaaagat aaagataagg
    30181 ctgataattt tatttttatt ggcagcactg tcatttattg gtcttttttt catcattaac
    30241 tatcaactgg tatcggaacg cgcggtaaaa cgtgccgata gccgctttga acttattcag
    30301 aaaaacgttg gctatttctt taaagatatt gaacgttcgg ccctgacatt aaaggactca
    30361 ctgtatttat taaaaaatac agaggagatt caacgtgccg tgattctgaa aatggaaatg
    30421 atgccatttt tagactcggt gggactggta cttgatgata ataaatatta tctcttttcg
    30481 cggagggcga atgataaaat cgttgtttat catcaggaac aagtaaatgg accgcttgtc
    30541 gacgagtcag ggcgggttat ttttgccgat tttaacccat cgaaacgacc gtggtcgatg
    30601 gcttcagatg actctaacaa cagctggaat ccggcataca attgctttga tcgtccgggt
    30661 aaaaaatgta tctcttttac gctacacatc aacggcaaag atcatgattt gttagcggta
    30721 gataaaattc atgtcgattt aaactggcga tatctgaacg agtatcttga tcacatcagc
    30781 gctaatgatg aagttctatt tttaaagcaa ggtcatgaga tcattgctaa gaatcaactc
    30841 gctcgtgaaa aattgattat ttataatagc gaaggtaatt ataatattat tgattctgtc
    30901 gatacggaat atattgaaaa aacatcagcg gtgccaaaca acgcattatt cgaaatctat
    30961 ttttattatc ctggtggtaa tttattgaac gcatcagata aactttttta tctgccgttt
    31021 gctttcatta ttatcgtatt gctggtggtt tatttaatga ccactcgagt gttccgtcgg
    31081 caattttctg aaatgacaga tttggtcaat acgctggcgt ttttgcccga ctctacggat
    31141 caaatcgagg ctctgaagat tcgtgaaggc gatgcgaaag agattatcag catcaaaaat
    31201 tcgatcgcgg aaatgaaaga tgctgaaatt gaacgggcaa ataaattgct ctcgctgatc
    31261 tcttacgatc aggaaagcgg ttttattaaa aatatggcga ttattgagtc caacaataat
    31321 cagtatctgg ctgtggggat catcaaactg tgtggtctgg aagctgtgga agcggtgttt
    31381 ggtgttgatg aacgcaataa aatcgtcagg aaattgtgtc agcgaattgc cgagaaatat
    31441 gcgcaatgct gcgatatcgt gacatttaat gccgatctct atttactcct gtgccgggaa
    31501 aatgtacaga cgtttacccg taagatagcg acggtaaacg attttgacag cagttttggc
    31561 taccgcaatc tgcgcatcca taagtctgct atttgtgaac ctttgcaggg ggaaaacgcc
    31621 tggagttacg cagaaaagct gaaactggcg atttccagta tccgcaacca tatgttctca
    31681 gagtttattt tctgtgatga tgcgaaactc aacgaaatag aagagaatat ctggattgcg
    31741 cgtaatattc gccatgcaat ggaaattggc gaactattcc tggtctatca accgattgtt
    31801 gatattaaca cccgcaccat tttgggcgcg gaggcgttgt gccgttgggt gtctgcggag
    31861 cgggggatta tttcaccgct gaagttcatt accattgctg aagatatcgg gtttatcaat
    31921 gagctgggct atcaaattat taaaaccgcg atgggtgaat tcagacattt tagtcagcgt
    31981 gcgtcgctga aggatgattt cttactgcat attaatgttt cgccctggca gttaaacgaa
    32041 ccacactttc atgagcgttt tactaccatc atgaaagaaa atggcctgaa ggcgaacagc
    32101 ctttgtgttg agatcaccga aaccgtgatc gagcgaatta atgaacattt ctatctcaat
    32161 attgaacaac tgcgtaaaca aggggtacgg gtatcgattg atgactttgg caccggtttg
    32221 tcaaacctga aacgttttta tgaaattaat ccagacagta taaaggtgga ttcgcaattt
    32281 accggcgata ttttcggtac tgcaggaaaa attgtgcgca ttattttcga tctggcacgc
    32341 tataaccgga tcccggtgat tgcggaaggc gtagagagcg aagacgttgc gcgtgaatta
    32401 atcaaattag gatgtgttca ggctcagggg tatctgtacc agaaacccat gccattctcc
    32461 gcctgggata aaagtggaaa attagtaaaa gagtagttta cgtatgtcca gaatcaataa
    32521 attcgtactt acagtcagtc tgctgatttt tatcatgatt tcagcagttg cctgcgggat
    32581 ctacactcaa atggtaaagg aacgggtgta tggcctgaaa cagtccgtta ttgatactgc
    32641 ttttgcggtg gcaaatattg ctgaatatcg gcgtagtgtg gcaattgatc tcatcaacac
    32701 gctaaatcct acggaggaac agctgttggt tggcttacgc acagcttacg ccgactcggt
    32761 ttcaccctct tatttgtacg atgtcggtcc ttatctgatt tccagtgacg aatgtattca
    32821 ggtaaaggag ttcgagaaaa attattgtgc agatattatg caggttgtta agtatcgaca
    32881 tgtcaaaaat acagggttta tctcttttga tggtaaaacc ttcgtctatt acctctatcc
    32941 ggtaactcac aatcgtagtc tgatattttt gcttggtctg gaacgttttt ctttactgtc
    33001 aaaatcgctg gcgatggata gcgagaacct gatgttctcc ctgttcaaga acggtaaatc
    33061 ggtgaccggt gatgaatata acgctaaaaa cgccatcttc accgtttcgg aagcaatgga
    33121 gcacttctcc tatttgccga ccgggttgta cgtatttgct tataaaaaag acgtttattt
    33181 tcaggtatgt acattgatta ttttctttgc cgcattggtg gcagtgatat cgggtgccag
    33241 ttgcctctat ctggtacgca gagtgattaa tcgtggtatt gtggagaaag aagccattat
    33301 taataaccat tttgaacgcg tactggatgg cgggcttttc ttttcggctg ccgatgtcaa
    33361 aaaactctac agtatgtata actcggcatt cctggacgat ctgaccaaag cgatgggcag
    33421 aaaatccttt gacgaagatt taaaagcgct gccggaaaaa ggcggttatt tgtgcctgtt
    33481 tgacgtcgat aaattcaaaa acattaacga caccttcggt catttgctgg gcgatgaagt
    33541 gttgatgaaa gtggtgaaaa tccttaaatc acagatcccg gtagataaag gtaaagttta
    33601 ccgcttcgga ggtgacgaat ttgcggtgat ttatactggc ggcacgctgg aagagttgct
    33661 atcgattcta aaagaaatcg ttcatttcca ggtgggaagc attaatttaa gcaccagtat
    33721 cggtgtagcg cattcaaatg aatgtactac cgttgaacgt ttgaaaatgc tggcggatga
    33781 gcggctgtat aagagtaaga aaaacggcag ggcgcagatt agctggcagt aatcattatt
    33841 cgcaggccgg acaaatgatt ttgcccggcc tgaattaatt aaacccggct accccacaaa
    33901 tcgtactcat cggcgtgctc gactttcact cgcaggatat cacccggctt aacgttggtt
    33961 tcaccattga gataaaccgc gccgtcgatt tccggtgcat ctgccatgct gcgaccaatc
    34021 gcgccttctt cgtccacttc gtcgataatc accagaattt cacggcccac tttctcttgc
    34081 aggcgctcgg cggaaatttg ctgctgcaac tgcatgaaac ggttccagcg ttcttctttc
    34141 acttcttccg gaacctggtc aggcagggca ttggcgtctg caccttcaac cgggctgtat
    34201 ttaaagcagc caacgcgatc cagacgtgct tctttcagga agtcgagtag catctggaaa
    34261 tcttcttccg tctcgccagg gaagccaaca ataaaggttg agcgtagggt cagttccggg
    34321 cagattttgc gccactgttt gatgcgcgcc agttggcgat ctacagaacc cggacgtttc
    34381 atcagtttga gaatgcgcgg gctggcgtgc tgcaacggaa tgtccagata cggcaggatt
    34441 ttgccctctg ccatcagtgg gatgacgtcg tccacatgcg gataagggta aacgtagtgc
    34501 agacgtgtcc agatccccag tttcgataac tgttcgcaca ggctgaccat gctggttttt
    34561 accggctcgc cgttgtggaa gccagtacga tgtttaacat cgacgccata cgcggaagta
    34621 tcctgcgaga tcaccagaat ttctttaacg cccgcatcaa ccagacgttt cgcttcactt
    34681 aacacttcgc caatcggacg gctcaccaga tcgccgcgca tagacggaat aatgcagaag
    34741 gtgcagcggt gattacagcc ttcagaaatt ttcagatagg cataatgacg cggcgtcagt
    34801 ttcacacctt gttctggcac caggctcagg aatgggttgt gtttcggttt tggcacgtag
    34861 tgatgaacgt gctccagaac ctgctcatag ctatgaggcc cggtgatttc cagcactttc
    34921 gggtggactt cgcggatctg atcttctttt gcccccagac aaccggtcac aattaccttg
    34981 ccgttttcat tcaacgcttc accaatggct tccagtgatt cttgtaccgc gctgtcaata
    35041 aagccgcagg tgttgacgat caccatgtcc gcatcatcat agctcggtac cacgtcataa
    35101 ccttcagtgc gtagttcggt gagaatacgc tctgaatcga caaggttttt cggacagcca
    35161 agggaaacaa agccgatttt cggctgggga gttactttgc tcatagctta aaaaatattc
    35221 agttacagga aaggtcaggg cagggattct acagagttct ggataaaatt tgtatcgcaa
    35281 tctcattcgc tggcggagtt gaaggaaatg taaattttgt taattcggcg tgaagaattg
    35341 atcctggaca gcattttgct caaaaaacag ccatactatt taattgcaac aaggctggaa
    35401 agaggaggat cgaagtatgt tcgttgacag acagcgaatc gatctgctga accggttgat
    35461 cgacgcacgc gttgacctcg ccgcatacgt gcaactgagg aaggcaaaag gatacatgtc
    35521 cgtcagcgaa agcaatcatc tacgagataa cttttttaaa ctgaatcgcg aactgcacga
    35581 taaatcgctg cggttgaatc ttcatctgga tcaggaagag tggagtgctc ttcatcatgc
    35641 tgaagaagca ttagcgacag ccgcagtatg tttgatgagt gggcaccatg attgcccgac
    35701 tgttattacc gtcaacgccg ataagcttga aaattgtctg atgagcttaa cgctgagtat
    35761 ccagagcctg cagaagcacg ccatgcttga gaaggcctga aaactaaggg ggagaaagcg
    35821 tctccccctt catgtttaag tttttgtaaa aatgaatttg ttatctcctc cactgactac
    35881 gctttaagcc agagtcaatc cggaggcgtt atgcatcgac aatccttttt ccttgtgccc
    35941 cttatttgtc tttcttccgc tctctgggca gctcctgcaa cggtaaatgt cgaagtactg
    36001 caagacaaac tcgaccatcc ctgggcgctg gcctttttac ccgataatca cggtatgtta
    36061 atcactctgc gcggcggcga gttgcgtcac tggcaagcag gaaaaggatt atctgcgccg
    36121 ctttccggag tgccggacgt ttgggcgcac gggcagggcg gtctgctgga cgtggtttta
    36181 gcgcccgatt ttgctcagtc gcgccgcatc tggttaagtt attccgaagt tggcgatgat
    36241 ggcaaagccg gaactgctgt gggttatggc cgcttaagtg atgatctctc aaaagtgacc
    36301 gacttccgca ccgtctttcg tcagatgcca aaactgtcta ccggcaacca ttttggcggg
    36361 cggatggtat tcgacggtaa aggttatctt tttattgctc tgggcgaaaa taatcagcgc
    36421 ccgacggcgc aggatctgga taaattacag ggcaaactgg tgcgtctgac cgacctggga
    36481 gaaatcccgg atgataatcc ttttataaag gaatccggtg tgcgcgccga gatctggtct
    36541 tatggcattc gtaatccgca aggaatggcg atgaatccgt ggagtaatgc actatggctg
    36601 aatgaacatg gcccgcgcgg tggtgatgaa attaatattc cgcaaaaagg caaaaactac
    36661 ggctggccgc tggcaacctg gggaatcaac tattcgggct ttaagatacc ggaagcgaaa
    36721 ggggagatcg tcgccgggac cgagcaacct gttttttact ggaaagattc gcccgccgtg
    36781 agcggcatgg ccttctataa cagcgacaaa ttcccccagt ggcagcaaaa attatttatt
    36841 ggcgcgctga aagataaaga tgtcattgtg atgagcgtca acggcgacaa agtgacagaa
    36901 gatggccgta ttttaacgga cagagggcag cgaattcgtg atgttcgcac tggacccgac
    36961 ggttatttat acgttctcac cgacgagtcc agtggggaat tacttaaagt tagcccacgc
    37021 aattagctaa cgggaatcat caccactttg cgcactgcgg ggcgttcagt gagttgctga
    37081 taccagcgtt gcagatttgg acgcggtgtc caggtcaggc caacgttaaa caaattgtag
    37141 atgaacggtg cgatagcgat atcgcccaca ccaaactcgt cgcctgagaa ccattttact
    37201 tttgccagtt ccgcatcgag cagggcaaac agggcgtcgc actctttgca actggcatca
    37261 atggcggcct gatcgcgctc ttccggtggt gttctgacta atcccatcag gatcccgcga
    37321 tgagcattac tgagcgtctg gtttgcccag tccatccatt tttctgcttc cgcacgacgt
    37381 gccggtgagt cgatccacag gcgtttttgc ccgtactgtg ccgccagata gcggacaatg
    37441 gcgtttgatt cccaaagaat gagatcactt tcgtcgtcac gcaacaacgg caccagcccg
    37501 ttagggttca tcgccagaaa atcagcatcg tgatttatcc caaactcacg gcccgcgaga
    37561 atttgctcat aaggtagttc cagttcttcg agcgtcagca atactttttt tacgttggtt
    37621 gaattattcc gaccccacag cgtaatcata ctcacccctt ttccgacagg cagccacagg
    37681 ctgaacagga ctccgatggt gaagtaaagt taacttttaa gcaacagctg gcaaaaaaat
    37741 gacaccagaa gcagcgaagc ggcaggatat tgcataaact ttaaaaactt taccaactta
    37801 cggtttcttt aagtttgtgt gtgcgttatt aatcaccgaa cttatcatac ggcgatataa
    37861 cgtatttttt tgaatggata ctcgggtggc atttatgacg caatactcct ctctccttcg
    37921 tggtcttgca gcgggttctg catttttatt cctttttgcc ccaacggcat tcgcggcgga
    37981 acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg attttaatgg attacgccag
    38041 cggtaaagtg ctggcagaag gcaatgcgga tgagaaactg gatcccgcga gcctgactaa
    38101 aattatgacc agctatgtgg ttgggcaggc gcttaaggcc gataagatta aactcaccga
    38161 tatggtgacg gtcggtaaag atgcctgggc gacgggaaat ccggcactgc gtggttcatc
    38221 ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca gacttgaaca aaggtgtgat
    38281 tatccagtcc ggtaatgacg cctgtattgc gctggctgat tacgttgccg ggagccagga
    38341 gtcatttatt ggtttgatga atggttatgc caaaaaactg ggtctgacca acactacctt
    38401 ccagacggtg cacggcctgg atgcgccggg gcagttcagt accgcgcgcg atatggcatt
    38461 gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac gccattcata aagagaaaga
    38521 gttcaccttc aacaaaattc gtcagcctaa ccgtaatcgt ctgctgtgga gcagcaatct
    38581 gaatgttgat ggcatgaaga caggaaccac ggcaggcgcg ggatataatc tggttgcttc
    38641 ggctacccag ggcgatatgc gtttaatctc cgtagtgctg ggggcgaaaa ctgaccgtat
    38701 ccgttttaat gagtctgaga aattattgac ctggggcttc cgtttctttg aaaccgtgac
    38761 gccaattaaa cctgatgcca catttgtgac tcagcgcgtc tggtttggtg ataagagcga
    38821 agtgaatctc ggggcaggcg aagcgggctc agtgaccata ccgcgtgggc agctgaaaaa
    38881 cctgaaggcg agttatacgt taacggaacc gcagcttacc gcaccgctga aaaaaggtca
    38941 ggttgtcggc accattgatt tccagcttaa cggtaaatcc attgagcagc gtccgctgat
    39001 cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt tcgtgatgat
    39061 gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc ggatggcgcg
    39121 aaacgtcatc cggttatacg tcattaatac atcaacttaa tacgctgcgt ctgcgcgtac
    39181 ttcacatact catcttccgg gcaacaatcg ctcacaacaa tatcaaagcg tttcaggtca
    39241 cccatgcgcg ccggacgcac cttgccaaat ttactgtggt cgacaaccag cacatgcttt
    39301 tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa gcaggtagcg
    39361 cctttactga catgcacgcc cgcagcggaa taaaaagcga tatccgggca aaaattattc
    39421 agcgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa ttcaccaccg
    39481 caaagaaacg cgcggcaatg gggtttctct ttcagcgcca gaaaagtatt tagcgaataa
    39541 caaacggcgg taaaagggat ttcattatca attgcttcaa taatccacgg cgtggtggtg
    39601 ccacagtcaa aaaagagggt ctgatcgggt tctatcagcg tcgcagccag ttttgccgcc
    39661 cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg gctggcgctg
    39721 cgtggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg gttgttcaga
    39781 tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt aagatgtaac
    39841 ttatcactgc gttttaattc ttgcagcagc tgcccgatac gctcttcgcg acgtgtttcc
    39901 ataatccctc tgaatagtta ttgaagcgag ccgctcaata ctacactttt tagcagagat
    39961 cagtcacgca cccagccttt gcggatcggt aatgcaaaac agaagcgata ccacgattgc
    40021 agacgttgat agagtttatg ccccatcgct tgccagataa tctgggcgct aaggcagcca
    40081 atcatacctg ccagcaatcc accgagcata tccagcggcc agtggacgcc aagataaacg
    40141 cgcgaccagg caatgacgac ggccagcacc attaaaagtg agccggacca caggcgatgc
    40201 cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac cgtgatcgct cgggaatgag
    40261 tcatccgccg catgatgcag gaagttatag ccgatatttt cgacaaaggg tcggtcgtgc
    40321 ggaaaaagat gtcccatcgt ccaggacaca aacaggctga ccgccagcgc gatagcgatt
    40381 tttatcacca gttgccgttg tgctgtaagc ccccacaacc aaagtactgc ggccagcaac
    40441 ggcaccacgg taatcaaatc tttagcaata aaaatcgcca acgagatcat ccacggcgca
    40501 gagtctggcg ttgcgttaat aagagagaat agagagagat tcaaattttc cagcataact
    40561 tcccgacgca aagtgattaa aagggagcca atacaggcaa gtcgttgaga ataaagtgca
    40621 ggttaaactg ggtaaagcgg catcgtctta tttccctcaa gcggcctgtt tacggtaggt
    40681 gattgtaacg ggcaaaggtt aaataaaact taaagaaagc gtagctatac tcgcaataat
    40741 gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg ctttctcgtt agctgcgctt
    40801 ttattaaact ttgcgcgatt attattggcg aagaaattgc atgcaaaata aattagcttc
    40861 cggtgtcagg cttggacgtc aggcgttact tttccctctc tgtctggtgc tttacgaatt
    40921 ttcaacctat atcggcaacg atatgattca acccggtatg ttggccgtgg tggaacaata
    40981 tcaggcgggc attgattggg ttcctacttc gatgactgcg tatctggcgg gcgggatgtt
    41041 tttacaatgg ctcctggggc cgttgtcgga tcgtattggc cgccgtccgg tgatgttggc
    41101 gggagtggtg tggtttatcg tcacctgtct ggcaatatta ctggcgcaaa acattgaaca
    41161 attcaccctg ttgcgcttct tgcagggcat aagcctctgt ttcattggcg ctgtgggata
    41221 cgccgcaatt caggaatcct tcgaagaggc cgtttgtatc aagatcaccg cgctaatggc
    41281 gaacgtggcg ctgattgctc cactacttgg tccgctggtg ggcgcggcgt ggatccatgt
    41341 gctgccctgg gaagggatgt ttgtcttgtt tgccgcattg gcagcgatct cctttttcgg
    41401 tctgcaacga gctatgcctg aaaccgccac gcgtataggc gagaaactgt cgctgaaaga
    41461 actcggtcgt gactataagc tggtgctgaa gaacggccgc tttgtggcgg gggcgctggc
    41521 gctgggattc gttagcctgc cgttgttggc gtggatcgcc cagtcgccga ttatcattat
    41581 taccggtgag cagttgagca gctatgaata tggcttgctg caagtgccta ttttcggggc
    41641 gttaattgcg ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg tacgttcgct
    41701 gattattatg ggtggctggc cgattatgat tggtctgttg gtcgctgctg cggcaacggt
    41761 tatctcatcg cacgcgtatt tatggatgac cgccgggtta agtatttatg ctttcggtat
    41821 tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata tgagtaaagg
    41881 tacggtttct gcggcgatgg ggatgctgca aatgctgatc tttaccgtcg gtattgaaat
    41941 cagcaaacat gcctggctga acgggggcaa cggactgttt aatctcttca accttgtcaa
    42001 cggcattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga tgggaaattc
    42061 tcacgaaggg taaaaaaatg cctgattgct ttgtgcgatc aggcattctc gaattaatgg
    42121 tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc aagatggcga
    42181 acataaggat gcgggcggta agccggagct ggcgcaggag ccacatagac ggtttgcggc
    42241 acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac aaccactttg
    42301 tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt aaagcccatt
    42361 aaaagcgcgc ccaacagtag acaatttttc ataatcattt cgcctgaaat aatgcagcgg
    42421 gttaatttcc gcgaattatg cagccttcat tcaggcattg tgttgccggg cgtgctgcct
    42481 tttttattgc ttaaatggcg cttcgtgttt aagcacttta tcgatcacat ccagtacgcc
    42541 ttcacggtta ttggagcctg cccggtattt tgccgctgcg acgaccgcgc tgccggcatt
    42601 ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac cgccatcgcc
    42661 aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc gaaggccatt
    42721 ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt ggaccggcac
    42781 cataatatcg ccgatggcct catgtaatgc tttttgtact tgtggaatca gttcatcgga
    42841 aagattcaga ccaaacttaa agaagatatc ttctaagttg tcaaagttat cgacgtattc
    42901 cagacggtga taatacattt ccgccaccgt tttcatggca tcgtcatatt ttttgagggt
    42961 ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca gcaaatgttc
    43021 cacgacagta gcaaacgcat ccttcgacag ctcgccatta aaaacatctt tgccttcgct
    43081 cactacccag ccgccgtttt cagccacaaa ggctatttca ttagcaattt cagggaagaa
    43141 agagatcaac tgataatatt gattcccgct ggcgaccaca aagcgaatgc cttgtgcttt
    43201 catttgctga tactgagcca taaaccgctc acggttatag gttttttgat cgcttaagaa
    43261 agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc catcgccgtc
    43321 tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag aataaccagc
    43381 gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata gcccagcagc
    43441 ggcggcccga cgaggaaagc cagataaccg gtcgtagcta ccacgctgac gcgggttggt
    43501 gcatcggggc cggtatcgct ggcggcagaa atagtcagcg ggaagcccaa cgaggcaccc
    43561 agtccccaaa gtacaacaga caccccagcg acccaggcgc tatcgacaaa aataatcagc
    43621 ccaataccca acgcccccat tagtgcactg gcccgaacca cggcaacgcg actgtaacgg
    43681 tcaatgaacc aaccgccggt aaagcgtcca acggtcatcc ccagggtaaa cccggcataa
    43741 atcagcgagc cggaagtagg gctaaaaccg tgaccatcaa ccattaataa gggtaaccag
    43801 tcgttggcag aaccttcggc aaaggccatc gccagcacca caacaccaat caacagcaac
    43861 tggatatccc gataaaaagg tacgcctttt tcaccatgct gggtgccatc ggcagcattt
    43921 ttgcccgtac cgtcagggat tgcctgaatg gcgatataaa taggtgcgat gcctaccagc
    43981 gccgccaata aaatatgcac cgttgccgga acgccaaagg ccgtaagtgc catcccgaca
    44041 cctgcgcctg ccagcgtgcc caggctataa aaaccgtgca tcatcggtaa aaccgtctta
    44101 ttcatttctc tctcaacggc ggcaccttca acgtttatcg ccacttccgc agaaccaaaa
    44161 ctagccccaa agacgccgag accaacggca aagagcaagg gcgatgtcag ccagagtgcc
    44221 agacttaata tcatcatccc aatcaatgcg caggacatcg tcacaaggat gacattacgc
    44281 gtcccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga accgatcgac
    44341 agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc gcggatagca
    44401 ggcgtacggg ttgcccagga cgccattaac aggcctggca aaaagaagaa cataaacagc
    44461 gcccaggttc ggcgtttcaa tgcattacgt gaagaattta cggtcataga ccacgtcaaa
    44521 ataagaagag ggaagacaac attagcaaga ctgtgtacat ttgtacacaa ttgcagagag
    44581 aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta tccaggccac
    44641 gctggaggcg gtgaaacttt acggaataca tgctgttaca caccgcaaaa ttgctaccct
    44701 tgccggggtg ccgttgggat cgatgaccta ctatttttca ggaattgatg agttgttgct
    44761 ggaggcgttc agccgtttta ctgagatcat gtcccggcaa tatcaggcat tttttagcga
    44821 tgttagtgat gctccaggcg catgccaggc tatcaccgat atgatctaca gctcacaggt
    44881 cgcaacgccg gataatatgg agctgatgta ccagctctac gcgctggcta gccgaaaacc
    44941 gctcttaaaa acggtaatgc aaaactggat gcagcgcagt caacaaacgc tcgaacaatg
    45001 gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gagggaatga cgctgcattt
    45061 tgtcaccgac cgtaagccgc tatcacgcga ggagattttg aggatggttg agaggattgc
    45121 tgggtagtag ataaatttca ggcaacaaaa aacccatcaa ccttgaaccg aaatggcggg
    45181 gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa ttaagactga
    45241 tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg caacttcagt
    45301 tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc agcaggacgt
    45361 tggcgattgc ataggtgccc gcatagccca gcgccgggat gttactgcga gctgtatcac
    45421 tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg aacaacagtg
    45481 cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccact ggcacaagac
    45541 tgacaatcaa tccggcaatc aacatctgac cgccaatcgc gcccaggccg ttattaatac
    45601 cgctaccggc gctcagacca acgcctgcca taaacaccat taagccgaac tctttcacca
    45661 tgcttaatgc accctgcgga atgtaaccga aggtcgggtg gttagcgcgc ataaatccca
    45721 gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg aatgtgctga
    45781 actggaaggt gatcatcccg atcatcagcc caataacaaa gaaggcgcag aatgccagca
    45841 agtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg gtttttacgc
    45901 gacgggcatc gccgctgact tgtaaaacgt cacctttgtt aagcacgacg ttgtcatcta
    45961 tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga tcggtcaact
    46021 tcagttgtgc gagacgtttg cccacggcgt tatggttttt aacgaccact tcttcagtga
    46081 cgatacgcat gtcgagaagg tcacgatcga aaacttcttt gccgttacgg aagctgggat
    46141 cgagtcgtgc gtgggcgtcg ggatagccta ccaacgctat ttcatcgccc atttgtagca
    46201 cagcatcacc gtctggattc gccagaatcc cgttacgtcg aatacgttca atgtagcagc
    46261 cagtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag gccaccagct
    46321 ccgggccgac gcggtaggcg cggatcaccg gtaaataaac cttacggttg gcatcagtgt
    46381 ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga tgctgcaatt
    46441 tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag gttaaggcat
    46501 acccgaggct cagattatcc agtgccagtg agagctgcct gctttccatg ccggaatgac
    46561 gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcca gctaacatac
    46621 cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg atcaccagcg
    46681 cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga aaaaaaatgg
    46741 aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata aagccaagat
    46801 taagcgcgtc ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat acgactaaaa
    46861 cgccaatgga attacccagt tggatcgaac caagtcgtaa tttcccgaga catagcccaa
    46921 gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg gcgacgttta
    46981 tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg gtaatttacg
    47041 ataatgtttt ttaccagaat tcagggcgca gattcattca gcgcacctaa acgatagtaa
    47101 agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata tgaattgcgt
    47161 tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca cactggcgaa
    47221 aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca acgttgggcg
    47281 ggggcaatct gctgttttgt cctcttcatt gtggtgtgtc tttttctggc gacgcacatg
    47341 aaaggcgctt ttcgggctgc cgggcatcct gaaatcggtt tgctattttt cattcttcct
    47401 ggtgcagttg ccagcttctt ttcacatcgt agagaagtcc tgaaacctct gtttggcgca
    47461 atgctggcgg caccctgttc gatgctcatt atgcggctgt ttttttcacc gacgcgctca
    47521 ttctggcaag agctggcatg gttattaagt gcggtgttct ggtgtgcgct gggggcattg
    47581 tgtttcttat ttatcagtag cttgtttaaa ccacagcaca gaaaaaatca ataaagccct
    47641 caacgcgagg gcttgtcaga cgatcaggcg tccagattct ctttcaccca tgcagcaaaa
    47701 tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt ttctacgggt
    47761 ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat atctacatac
    47821 tgatactgga aatcatcgcg ctcattgctc aatttctcgg ccagatcttt tgcacgcaca
    47881 cagtaagggc aacccggacg accaaaaata acggtttgca ttatttctct cctcatagat
    47941 ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa cctgttgcat
    48001 taattgctaa aagctataac tgttaaacac aatgcagtga aaagttttag actgaaggct
    48061 cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaagc gtgttgatac
    48121 tggaatttat cggaatgatg ttgctggcgg tggcgctgct gtcggtaagc gactccctgt
    48181 cgctacctga gccattttct cggccagaag tgcagattct gatgattttt ctcggtgttt
    48241 tgctcatgat tcccgctgcg gtggtggtga ttcttcaggt ggcaaaacgt cttgccccac
    48301 agctgatgaa ccatccaccg caatattcac gttcagaaag agaaaaagat aatgacgcca
    48361 accattgaac ttatttgtgg ccatcgctcc attcgccatt tcactgatga acccatttcc
    48421 gaagcgcagc gtgaggcgat tattaacagc gcccgtgcga cgtccagttc cagttttttg
    48481 cagtgcagta gcattattcg cattaccgac aaagcgttac gtgaagaact ggtgacgctg
    48541 accggcgggc aaaaacacgt agcacaagcg gcggagtttt gggtgttctg tgccgacttt
    48601 aaccgccatt tacagatctg tccggatgct cagctcggcc tggcggaaca actgttgctc
    48661 ggtgtcgttg atacggcaat gatggcgcag aatgcattaa ccgcagcgga atcgctggga
    48721 ttgggcgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac ggaactgctg
    48781 aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc tgcggataat
    48841 ccggatctta agccgcgttt accggcctcc attttggtgc atgaaaacag ctatcaaccg
    48901 ctggataaag acgcactggc gcagtatgac gagcaactgg cggaatatta cctcacccgt
    48961 ggcagcaata atcgccggga tacctggagc gatcatatcc gccgaacaat cattaaagaa
    49021 agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg ctaaaactgc
    49081 cacgtcgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg cagggtgaaa
    49141 attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg tgaagtcgct
    49201 atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat gaacataaat
    49261 cctgcggcgt cttctattca ctacaaaggc cgcaagttac cccattttga cgcagtgatc
    49321 ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca gttcgagatg
    49381 ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga caaattgcgt
    49441 tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat tgcgcattcg
    49501 ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt ggtcaagctg
    49561 gttgaaggca cgcagggaat tggtgtcgtg ctggcggaga cgcgtcaggc ggcggaaagc
    49621 gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata tatcaaagag
    49681 gcgcaagggt gcgatatccg ctgtctggtt gttggcgatg aagtagtcgc tgcgattgaa
    49741 cggcgagcga aagagggcga ttttcgttcc aatttgcatc gtggcggcgc ggcaagcgtc
    49801 gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac gatggcgctg
    49861 gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat ggaagtgaat
    49921 gcgtcgccag ggctggaagg aatagaaaaa accaccggta tcgacatcgc gggtaaaatg
    49981 atccgctgga tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg tggttagtcg
    50041 caatcacatt actgatcatg gttttgcctg cgctttttgc gtaagctgtg ccggtctttt
    50101 tatcggaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct ggatacgttg
    50161 cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg tcaggctctg
    50221 catctgcccc atatgcagaa tttcgacggt gtctttgatg ctaaaatcga tctgatcgat
    50281 aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt gccgctggcg
    50341 gcggatttat cagccatcaa tgccagttcg ctgaccgtga aagcatttct tgatatgcag
    50401 gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca gggcgtaacc
    50461 tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat ggtcattctt
    50521 gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa taacgttacc
    50581 gaaaactatt tcctccactg ataactcctt tcgagcacgc agtcgctgcc gcagtgactg
    50641 cgtgccgcaa aattatccgc tgtttttaac cttttattaa agattaattc acttctcttg
    50701 tgccgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa ataccgtttt
    50761 ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc ttttatatag
    50821 tgagcagtgc aggccgggag agagttctct tttcttacac cgcgccgata aaaatatgca
    50881 cgtttattgc atatctttca gtgtgacaac ttttgttcgt ttgttaacga attttcagaa
    50941 ggaaagagat atgaccgcct taaataaaaa atggctatcg ggtctggttg cgggggctct
    51001 gatggccgtc tctgtcagca cgctcgcggc tgaacaaaaa acactccaca tttataactg
    51061 gtctgattat atcgccccgg acacggtggc caactttgaa aaagaaaccg gtattaaagt
    51121 cgtctacgat gttttcgatt ctaacgaagt actggaaggc aaattaatgg cggggagcac
    51181 tggctttgat ctggtggtgc cgtcagcaag ctttcttgag cgccagctga cagcgggagt
    51241 tttccagccg ctggataaaa gcaaattgcc ggagtggaag aatctcgatc cggagctgct
    51301 gaagttggtc gccaaacacg atcccgacaa taaatttgct atgccctata tgtgggcgac
    51361 gaccgggatt ggctataacg tcgataaagt taaagcggtg ttgggcgaaa acgcgcccgt
    51421 cgatagctgg gacttgatcc tcaaacctga aaatctggaa aaactgaaaa gctgcggtgt
    51481 ctctttcctg gatgcgccag aagaagtgtt tgctaccgtg ctgaattatc tcggcaaaga
    51541 tcctaacagc actaaagcgg atgattacac cggaccggct accgatctgc tgttaaagct
    51601 gcgcccgaac attcgttatt tccattcatc ccaatacatc aatgatctgg cgaatggtga
    51661 catctgtgtc gctattggct gggcgggcga tgtctggcag gcgtcaaacc gcgcgaagga
    51721 agcgaagaat ggcgtgaatg tctcgttctc gattccaaaa gaaggggcga tggtgttctt
    51781 tgatgtattc gccatgcctg cggatgccaa aaacaaagac gaagcctatc agttcctgaa
    51841 ttacctgctg cgtcctgatg tggtggcaca tatctctgac catgtgttct atgctaacgc
    51901 caataaagca gccacgccgc tggtgagtgc ggaagtccgt gataacccgg gtatttatcc
    51961 gcctgcggat gttcgtgcga agttgttcac tctgaaagtg caggacccga aaatcgaccg
    52021 tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa taatccgcag ccgtagatgc
    52081 cggacgggcg caccacaccc gccggcaatt cgcaccatca tggtgcgctt gcacacattc
    52141 aatgccggag agcagccgtg aatgacgcta tccctcgccc gcaggcgaaa acccgtaagg
    52201 cgctgacgcc gctattagaa atccgtaacc tgaccaaatc ctacgatggt caacatgcgg
    52261 tggatgatgt cagcctgacc atctacaaag gtgaaatctt cgcgctgctg ggcgcatccg
    52321 gctgtggcaa gtccacgctg ctgcgtatgc tggcaggttt tgaacaacct tctgccgggc
    52381 agattatgct cgatggcgtc gatttgtcac aggtgccgcc ttacctgcgc cccatcaata
    52441 tgatgtttca gtcttacgcg ctgtttccgc atatgaccgt ggaacagaac atcgcttttg
    52501 gcctgaaaca ggacaaacta ccgaaagcgg aaattgccag ccgggtcaat gagatgcttg
    52561 ggctggtgca tatgcaggag ttcgccaaac gcaaaccgca tcagctttct ggtggtcagc
    52621 gtcagcgtgt ggccctggcc cgaagccttg ccaagcgccc gaaactatta ctgctcgatg
    52681 agccgatggg cgcgctggat aaaaagctgc gtgaccggat gcagcttgaa gtggtggata
    52741 ttctggagcg cgtcggtgtg acctgcgtaa tggttactca cgatcaggaa gaggcgatga
    52801 ctatggcggg gcgcatcgcc attatgaatc gtgggaaatt tgtccagatt ggcgagccgg
    52861 aagagatcta cgagcatccg accacccgct acagcgctga atttatcggt tcggttaatg
    52921 tctttgaagg tgtactcaaa gagcgtcagg aagatggtct ggtgctttat tcgccggggc
    52981 tggtgcatcc actgaaagtc gatgcggatg cttcagtggt tgataacgtg ccggtgcatg
    53041 tggcgcttcg cccggaaaaa atcatgcttt gcgaggaacc gcccgccaat ggttgtaact
    53101 tcgcggtggg ggaggtgata cacattgcct atctcggcga tctttcggtg tatcacgttc
    53161 gcctgaaaag tgggcagatg attagcgccc agctacaaaa cgcccatcgc catcgtaaag
    53221 ggttaccgac ctggggcgac gaagtacgtt tgtgctggga agtggacagc tgtgtggtgc
    53281 tgacggttta aggagcaaag atgagtacac ttgaacctgc tgcccagtcg aaaccgccgg
    53341 gcggatttaa gctgtggttg tcgcagctgc aaatgaagca tgggcgcaaa ctggtcattg
    53401 cgttgccata tatctggttg atcttgctgt ttctgctgcc atttctgatc gtctttaaaa
    53461 taagcctggc ggagatggcg cgcgctattc caccttatac cgaattgatg gagtgggctg
    53521 acgggcaact ttctatcact cttaatctcg gtaattttct gcaactgact gacgatccgc
    53581 tctattttga tgcttatctc cagtcgttac aggtggcggc gatttcgact atttgctgtt
    53641 tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg acccgtaata
    53701 tcttattact actggtgatc ctgccgtcgt ggacctcgtt tctgattcgt gtctacgcct
    53761 ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg ctgggggtta
    53821 tcgatcaacc gctgaccatt ctgcatacca actttgcggt ttatattggc attgtttacg
    53881 cctacgtgcc gtttatggtg ctgccgattt acaccgcgtt gattcgtatt gattattcgc
    53941 tggtagaagc ggcactggat ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg
    54001 tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc ccggctgtgg
    54061 gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc gggcgcgtgc
    54121 tgtggcagga gttctttaac aaccgcgact ggccggtggc ctcagcggta gcgatcatca
    54181 tgttgctgct gctaattatg ccgataatgt ggttccacaa acatcagcaa aaaagcgtgg
    54241 gagaacacgg atgaataatt taccggtggt tcgttcgccc tggcggattg tgattttgct
    54301 gttgggcttc acctttctct acgcgccaat gctgatgctt gttatctatt cgtttaacag
    54361 ctcgaagtta gtgacggtgt gggccggctg gtcaacgcgc tggtatggcg aattattgcg
    54421 cgatgacgca atgatgagtg cggttggttt aagtctgaca attgcggcct gtgcggcgac
    54481 ggcggcggcg atcctcggga ctattgcggc ggtcgtgctg gtgcgctttg gcaggtttcg
    54541 cggatcaaat ggttttgcct tcatgatcac cgcaccgctg gtaatgccag atgtcatcac
    54601 gggtttgtcg ctgttgttgt tattcgtcgc gcttgctcat gctattggct ggcctgcgga
    54661 ccgcggtatg ctcaccatct ggctggcgca tgtcactttc tgtacggctt atgtggcagt
    54721 cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagccg cgatggatct
    54781 cggtgcgacg ccgctgaaag tgttttttgt cattacgcta ccgatgatca tgcccgcgat
    54841 catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga tcgccagctt
    54901 tgtttccggg ccgggagcca ccactttacc gatgctggtc ttttccagcg tgcggatggg
    54961 ggtgaatccg gaaatcaacg ccctggcaac attgatcctc ggcgcggtcg gaattgtcgg
    55021 atttatcgcc tggtatctga tggcgcgcgc agaaaaacag cggatacgcg acatccagcg
    55081 tgcaagacgt ggctgaagac actaaaattt gccaacctgg ctacataatg ccgcgcatgt
    55141 cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac atcttcatct
    55201 catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg cgctcgccat tattatgatc
    55261 cgtggcctcg acgtgctgat gatttttaat acgctgggcg tgcgcggtat tggcgagttc
    55321 attcatcgca gcgtacaaac ctggagttta acgctggtct ttttgagcag tctggtgctg
    55381 gttttcattg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg
    55441 ctgtatctgt tgacacaaat cacagccgca agttacttgt gggcggcctc gctggggtat
    55501 ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt ccatagcctg
    55561 atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc ctcgactagt
    55621 cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca
    55681 atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca
    55741 gccgattcca gagcaactct ccgctaaaac cgtcgatctt aaaaatctgc tcgccgattt
    55801 tccggttgag gaatggtgtg cgccggtgtc aggcccggaa caagggtttc gtaataaagc
    55861 caaaatggtg gtgagtggta gcgttgaaaa accactgctc ggtatgctgc atcgagatgg
    55921 tacaccggaa gacctttgtg actgcccgct ttatccagcc tcatttgcgc ccgtttttgc
    55981 ggcgctaaaa ccgtttatcg cccgtgcggg gttaacgccc tacaacgtgg cgcgtaaacg
    56041 tggtgaactg aaatacattc tgctgactga aagccagagc gatggcggca tgatgctgcg
    56101 ttttgtactg cgttctgata ccaaactgac gcaactgcgt aaggcgctgc cgtggttaca
    56161 ggaacaatta ccgcagctga aagttattac cgtcaatatt cagccggtac atatggcgat
    56221 tatggaaggg gagacggaga tctacctgac cgaacaacag gcactggcag agcgttttaa
    56281 tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg cggtcgccag
    56341 ccagctgtac gccaccgcgc gcgactgggt acgacagctg ccggttaaac atatgtggga
    56401 tctgttctgc ggtgtgggtg gctttggttt acactgcgcg acgcctgaca tgcagttaac
    56461 cgggatcgaa attgcaccag aggccattgc ctgtgcgaag cagtcagccg ctgaactggg
    56521 cttaacgcgt ttgcaatttc aggcgctgga ctccactcag tttgccaccg cccaggggga
    56581 agtgccggag ctggtgctgg ttaacccgcc gcgccgcggc attggtaaac cgctgtgtga
    56641 ttatctctca acgatggcac cgcgttttat tatctactcc agctgtaacg cccaaaccat
    56701 ggcgaaagat atccgcgaac tgcctgggtt tcgcatcgaa cgggtacagc tttttgatat
    56761 gttcccgcac actgcgcatt acgaagtgct gatactgctg gtgaagcaat aaaaaagccg
    56821 caggtgcggc tttctgaatc ttactgtggg aaccactggt cattgatttt ctgatatgta
    56881 ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc tttgttatcc
    56941 ggacgtacag cgatacccag gccggtgccg aagtattgcg gatcggtcac tttttcagta
    57001 gcaacgccca gttgcggatt ggttttcagc cattcgttta ccactgcggt gtcaccaaat
    57061 acgccatcaa tacggccatt tttcagatcg ataaaggcat tctgataact gtcataggag
    57121 acagttttca cttccgggtg ctgatcctga atatatttct ggtgcgtggt gccgttttcc
    57181 atcccaatac gtttgccttt cagatcggca aacgttttgt aggtatcttt tttggcaatc
    57241 acgacggctg agttttcgta gtagggcgtg gtaaacgata cctgtttgct acgctccggc
    57301 gtgatatcca ttccggagat tacggcgtca tattttctga atttcagtga cgggatcagg
    57361 ctgtcgaacg cgtgattagt aaaagtacat tctgcctgca tttgtttgca caaggctttt
    57421 gccaggtcga tatcaaagcc gacaatctca ttattagcac ctatagattc aaagggtgga
    57481 taggtggctg aaacgccaaa attgattttc tctgcggcag aagcaccgaa agtaaaggaa
    57541 gtaagtaatg cggcaagaac taactttttc atgatggaac tcccgtctgt caatcttatg
    57601 atttttggcc gtgtctgcgg catgggataa caatgccatt aagtgaattt atatgcaata
    57661 aatatgagta aataatttat atgaaataaa aaaagacgga cagattagtg ggttgcccgt
    57721 cttcataata agaatttatg cactatgtag gccggataag gcgtccacgc cgcatccggc
    57781 atcagcatgc agctacatta attccgccgt tcgaatgcca gtgctttacg ctcgatcaga
    57841 cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccccgc cgcgccaaat
    57901 accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac ttccatcagc
    57961 gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt ggaataagaa
    58021 gagagcgagc gtttaaacgc gtacggcagc aggatcgcca gcgtatcttt tttgctcatt
    58081 cccagggcgc tacaggactg ccattgacct tccgggatcg cgcgaatcgc accgtaaaac
    58141 agctgcgtgg tgtacgccgc actgttcagc gacaacgcaa tcagcgcaca taaccacggt
    58201 tctgacaaca aatgccacag tgccggatac tcctgcaacg ttgggaactg gcccggtccg
    58261 taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt gatatagccc
    58321 cgcaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc
    58381 agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg cagccctttc
    58441 atcagttcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca
    58501 ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa atcaccgccg
    58561 ccacaatgta ccaggtaaac ggttcctggg tacgggtagc gatgcttttg gtttgtagca
    58621 ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc accagccact
    58681 ggttaccgag acccggcagc gcatgacgcc acatctgcgg catcaccaga cggaaaaaga
    58741 tagccgattt cgacagcccc agcgcctgac cggattccca ctgacccacc ggcaccgctt
    58801 ttaatgcacc gcgcagcgtt tgcgaggcat aggcggcata cagcagtgac agagcgatga
    58861 caccacaaag gaacgggcta acgtcgaagt tctcaatgtc catctgcacc gggatctgca
    58921 cgaacccaag attgatagtg aagccatccg aaagcgtcag cagcagctgc gaggagccaa
    58981 aatagataaa cagcaccacc agaatttctg gcaggccacg cagaatggtt accagcgctg
    59041 aacctgccca cgcgacagga cgccatttcg cagactccca taccgcaaag aacatcgcca
    59101 gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg
    59161 ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg tagatggttt
    59221 cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gagtttctgc tgcagttcag
    59281 tgttgccctg gcgtactgcg atgccgaggc cagtaccgaa gtaatcttta tcggtcactt
    59341 tatcgcccac tgccgccagt ttcgggttat ctttcagcca ttctgttacc actgcggtgt
    59401 caccaaatac gctgtcgata cgaccgtttt gcagatccaa ttttgcgttc tggtaactgt
    59461 catatggcac ggtagtgatt tccggatgct tatccataat gaacttctgg tgtgtcgtcc
    59521 cgttctgaac gccgactttt ttgcctttca gctgatcaac actggtgtat ttgccttgct
    59581 gacccacaaa cagggcagag ttgtcatagt acggggtggt aaacagcacc tgcttttcac
    59641 gctccggagt gatatccatg cccgccatca ctgcttctac gcgacggaat ttcaggcttg
    59701 ggatcaggct gtcaaacgcc tggttagaga aagtgcaggt tgcatcaatc tctttacaca
    59761 atgcctgcgc caggtcgaca tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa
    59821 acggaggata ggaggcttcg gtggcaaaac gaatggtttc ggctgctgtg gcggaaagac
    59881 taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc ccgaatctta
    59941 gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc
    60001 ttgttctacg atatgaccgt tttccatata caccactcgg ctggcggttt tacgcgccac
    60061 ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct cacgaatgat
    60121 gctgacgatt tgtgccgtaa tttcggggtc cagtgcggcg gtcggttcat cgaacagcag
    60181 tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct gacctccaga
    60241 aagatgcagc gggtaacgat cgctataagg tttgagacgc agacgttcca gcagtttttc
    60301 tgcacgggcc agcgcctgat ctttactcaa ccccagtaca cgacagggcg cttcaatcag
    60361 gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca tgccaacgtt
    60421 acgacgcaaa tcgcgaatcg ctttgtcaga gggggttttg gtgaaatcga aatggttgcc
    60481 tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac gcagcagcga
    60541 gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag
    60601 cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat
    60661 actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga tagtaccttt
    60721 gacagaataa ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc attattcaat
    60781 aaacatagca caaaataacg ggggcggtgg tcggcgagca taaatgtcgg cattcctcac
    60841 gaaatgccgg acaatttacg gggtttattg gttgatcaag acgttagcga ttctcgatgg
    60901 attgacggag cgtacccgcc gtggcatgaa cgctaccgcc taagtagcgc acatcgtcga
    60961 tgacccaaca ctggccttcc tgaatcatta acacttcatc ctgccagccc tggtcaccct
    61021 gcttgagatc cacgcgcaat ggaatgttac gggcatcacg attagggata gtagatgcac
    61081 tggcaacgtg ggcgctgtct ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca
    61141 attcacgatg actattatcg cggctggcat cgctaagtag ggttgccagt ttgtcactca
    61201 aataggggcg cagagcggta atgtcattgc tgcgatgttg aatgtggtag tcatagaact
    61261 gttgagccac gctatcaggc cccccctgga cacaggaacc ggtgcggggg ccgttatctt
    61321 tataagctgg cgtgacggtg gtacaggcac tgagtagcag tgcgcaaggg ataagcatgg
    61381 tcaatttgct atagcgcatg atgatttcct tataagcgaa caccttgaaa gcgttctacg
    61441 ataataatga tatcctttca ataatagcgt atcagtccga taatgctttg aagatcgaag
    61501 gcttagcaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct ggaaggccag
    61561 accatcgttg aatattgcgg tgtggtgact ggcgaagcga ttttaggcgc gaatattttc
    61621 cgtgatttct ttgccggtat ccgcgatatc gttggcggac gctccggtgc ctacgaaaaa
    61681 gaactgcgta aagcgcggga gattgccttt gaggagctcg gctcccaggc gcgagcgctg
    61741 ggggctgatg ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca aaacggcagt
    61801 atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct
    61861 ggctggtcgc tgccgctctg ttactggcag ggtgcacagg cgaaaaaggc attgtcgaga
    61921 aagagggata tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag
    61981 tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca ctgaccgata
    62041 agcaggtcag ctcgcattat ctggtccctg cggtgccacc gcgatacaac ggtaaaccgc
    62101 gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc
    62161 gcggggcaac gcgccttaac gacacctcta ttggtattga gctggaaaat cgtggctggc
    62221 agaaatcggc gggggtgaaa tattttgccc cgtttgaacc ggcacagatt caggcgctta
    62281 ttccgctggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtagcac
    62341 atgcggatat tgcgccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac
    62401 tggcacagca ggggattggt gcctggccgg atgcgcagcg ggtgaacttt taccttgccg
    62461 ggcgcgcgcc acacacaccg gtagagaccg cgtcattgct ggagcttttg gcgcgttatg
    62521 gttatgacgt aaagcctgat atgactccgc gcgagcagcg gcgcgtgatt atggcgttcc
    62581 agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg
    62641 ccgaagcgtt gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgta
    62701 gccaggcggc agttttctcg ataccttcat ccagggtgag gactggctga taacctaact
    62761 ctccctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag acgccgtagt
    62821 gggtcagcgg cggctcttta gctgacttgc gacctaaacg ctccatgctg cgggcgatca
    62881 tatccagcat cgggtaagga accgaacgaa tacgacagtc aatattcaac tcgtcgatca
    62941 gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc
    63001 cggaaggtag cttatcgcac gcttcctggc ttgccagcca catggcgtgc acggcatttt
    63061 catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata ctgccgtagt
    63121 gatgcatcat atgcgccaga cgggggataa agactttatc gtgcggtccg aacagacttt
    63181 gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt
    63241 cttcactggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt
    63301 ctttaatatc gcgatggtgg tgataatcga agtacaggga gggagaagag atatgaataa
    63361 agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg cgaacgttag
    63421 ccagatcgaa agcctgttgt gtcccccagg gcgaggtaaa gctggagcag tgccacagcg
    63481 tatcaatgcc cgcgagcatc actttagctt gtgatgagac cagctcggtc agatccgccg
    63541 gaacaaactc tgcgcccatt ttttccagca atttacccat tgcctcattg cgaccggtcg
    63601 ctcgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg
    63661 tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca
    63721 ttcttccgtg atttccccct tgatgcaatg ggaaacatga aagaataacg caggttttgt
    63781 cgattaatct gtgctttgtt ctgccagtct ggcgattcgt tttgccattc cgcgaaaaat
    63841 aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg
    63901 ccagaaagca cggacatcaa tagtacgata gtcgccttta tcttccaggg taaaacacag
    63961 tcgtcccagc cccggcgctt tcatgccaaa taacaacgta agttgttttt ccggttcaac
    64021 gacaatcact ttccagctat ccaccgcatc gccggtctgt aaatattcgc gctccgggcg
    64081 gcctttcgcc agcttatgac cgatcgcaag gtccatcaac gcccgtgtct gccacaaaat
    64141 attgccaaag aaataacgct ctttaccgcc gatttgattg accacccgcc atagcgcctg
    64201 aaggctggca gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac cgtattctgg
    64261 tcgccagcga gcaaaagcct gcgcgtcata tccccagtcg ctggagttga ccagtttttc
    64321 ttcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag
    64381 cgcacgtagc gcggtgtcat ccgccagcag atcgtgtttc agcccctgaa tcaacgccct
    64441 ggcggtggtg ggcggtactg aagtaatcac attaagaaac cacaccgaaa tccagcgggt
    64501 ggggaggggg atggggagca accagcggcg cttaccgctc accgccataa aatgttcaaa
    64561 ctgttgctga taactgagca cttctggtcc ggcggcttcg aagatgcgat gttcgcaggc
    64621 cggatgatct aacagcgcca ccagatagtg cagcaagttt tccagcgcga tgggcgtggt
    64681 gcgtgaacga acccagcgtg gcggcgttaa cacgggcaag ttgtaaacca tatcgcgcat
    64741 gacttcgaat gccgctgaac ccgcgccaac gataattccg gcacgtagtt cggtcaccgg
    64801 gacacccgct tcacgtaaaa tatccgccgt ggcctgacga gcacgcagat gatcagattg
    64861 ttcatgaggc ggcgcctgca atgaactgag aaagattaat tgctttaccg gtacttcgcg
    64921 tagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat caccgccttc
    64981 gcccatgctg tgcaccagaa aatagacagt atcaatattc tgcaacaggg ccggaaggtt
    65041 atccggccag ttgagatcga ctttatggca actgacatta gccagttgca gttttgcaag
    65101 tctgtcgaca tgacgtgccg ccgccaggat ctgatgccct tgctggctga gcgtgcgcac
    65161 cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg gcacgtctct
    65221 ctccttaacg cgccaggaac gcatgccagt gggcggcaac ttccgccagt tgttcacgcg
    65281 agacgtcaag atgggtcacc aggcggacaa tcggcgaggc gttaatcagc acgtttctcg
    65341 ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca aactgcatat
    65401 tggtgtcctg acgcatcaca tccgcgcctg cttcacgcaa ctgctccgcc atccaggtgg
    65461 cattgtcgtg gtcttcctgc aaacgtgcga cgttattttt cagggcatac atcccggcgg
    65521 cagccagaat gccggactgg cgcatcccgc cacctgtcat tttccgccag cgaatggcac
    65581 gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca agacctttcg
    65641 acaggcaaat ggtgaacgag tcacaatatt gcgtgatctc tttcagttcg cagccgtaag
    65701 ccaccacggc attaaaaatg cgcgcgccgt caacgtgcag cgccagattg cgctcgcggg
    65761 taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg tgggtgtttt
    65821 ccagactgag taatttggtg cgggcgaaat ggatatcgtc aggtttgatt ttcatcgcca
    65881 ctttatccag tggtagcgtg ccgtcggaag ctgcgtcgat gggttgcggc tgaatactgc
    65941 ccagcactgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga ccgacgatat
    66001 actcttcgcc gcgttcgcag tgactgagca gagcgaccag gttggcctga gtgccggtcg
    66061 gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc agagcattaa
    66121 cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatc gcttcgagca
    66181 tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca tgtccttatt
    66241 atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt tcgatcactt
    66301 catcaccgcg cccgctgatg attgcgcgca gcatatacag gctgaaacct ttggcctgtt
    66361 cgagtttgat ctgcggtgga atggctaatt cttctttggc gaccaccaca tccaccaaca
    66421 ccggaccgtc gatggagaag gcgcgttgca gagcttcatc gatttcagac gctttttcta
    66481 cacgaatacc cgtaatgccg caggcttcgg caattcgggc aaagttggtg tcgtgtagtt
    66541 cggtgccgtc agtcaaatag ccaccagctt tcatctccat cgccacaaag cccagcacgc
    66601 tgttattaaa gacgataatt ttcactggca gcttcatctg cactaccgag aggaaatcgc
    66661 ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctggcgt tctggctcgg
    66721 ttgcctgcgc gcccagcgcc tgcggcatgg cgttagccat cgaaccatgg ttaaacgaac
    66781 ctaacaggcg acgcttgccg ttcattttta gataacgtgc cgcccacacc gttggcgtac
    66841 caacgtcaca ggtgaaaata gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat
    66901 attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct ttgcgggcgt
    66961 cgcggtaatc ttccagcgcc ttatccagaa aattgcgttc ggttttttct tccaccagtg
    67021 gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct accttgctat
    67081 gcgcgccgat gctggctggg ttgatatcaa tctgaataat tttggcatcg gtcgggtaga
    67141 acgcacggta gggaaactga gtaccgagca gcactaacgt atcggcgttc atcatggtat
    67201 ggaaacctga cgaaaagccg attaatcccg tcattccaac atcatatgga ttatcgtatt
    67261 cgacatgttc tttaccgcgc agggcatgaa ctataggcgc tttaattttc ccggcaaact
    67321 caactaactc tttatgcgcc cccgcacagc cgctgccaca catcagggcg atattgctgg
    67381 aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggtgtt actaccggct
    67441 gtggcgcatg ataccagtgg gtagttgccc cttctggcgc aggttttaac gccacatcgc
    67501 ccggtaacac aacaacggaa acgccacggt taagcaccgc tttgcgcata gcaattgcca
    67561 gcacttgtgg gatctgctct gggctggaaa ccagctcgca atagtgacta cattcgcgga
    67621 atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg gagggaatat
    67681 gagcggcaat cgccagtacc ggaacgtgat tgcggtggca atcgaacagg ccgttgatta
    67741 agtgcaggtt gccgggaccg cacgatccgg cacagaccgc cagttctccg ctaagttgtg
    67801 cttcagcgcc agcggcaaat gccgccactt cttcatgccg ggtggacatc cactcgatgg
    67861 tgcccatgcg attaagactg tcactaagac cgttcagaga gtcgcccgtg actccccaga
    67921 tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc gtttgtttca
    67981 tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg acggaggggg
    68041 aagggatggg agaaaaagga ggcactaacg gttaaatagc ccgatgaaag gaatatcatc
    68101 gggcatcagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag caggccagta
    68161 cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt gctgctcacc gtatattcac
    68221 cggaaatcac tttcgtcttg cagcagccgc aaacacccgc acggcaggcg gcgacaaccg
    68281 gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggagcg taaaattctc
    68341 gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttccgct actggggtga
    68401 agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat
    68461 ccatatatgg agcagggcca caggtcatca cggtacgtga ggctaaatca gggacacctg
    68521 ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta ttttccgcca
    68581 ccagcgttac cggatagtta cgccactcat cggcgaaaat cacatcctgc ggcgtacgca
    68641 cgttgtagat cacctgcaca tcggcctgtg gacggttctt cgccagccag cgacgcatcg
    68701 acataatcgg cgtaacgccg cagcctgccg ccagcaacag gaatttatct tctgctttat
    68761 cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg cgttttacat
    68821 cgcgcgtcag ccaccgggag ccgacaccgt catcaatccg ccgcacggtc agggtgatgt
    68881 attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc gctgagttac
    68941 gcacgctgac cagcgcatat tgcccggcgc gatacgggta gtaatcgtgg caaatcaggg
    69001 aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc cgccacgggc
    69061 attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc
    69121 ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc
    69181 tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacac ccagagaaag
    69241 cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca gcgacagcgg
    69301 cagatcgttg actccgcagc ccagtttctc tgccagcgtt accgccagaa taattgctga
    69361 gtaagcatcg ttacactgac cagcatctac cagacgcggc agaccttcga tatcgccaaa
    69421 ctcaagttta ttaaagcgat atttaccgca agcgagggtc aggatcaggc agtcatccgg
    69481 cacgctggtg gcgaaatcgg tgaagtagtg acgctcgccg cgtgcgccgt cacagccgcc
    69541 aagcaggaag atatgacgca gtttttcacg gctcaccaga tcaatcagcg tatcggctgc
    69601 gccaagcagt gtctggcgac caaagcccac ggtgatcagg tgcggaattt cgctgtacgg
    69661 gaagcccgcc atctgttgcg cctgggcaat gactgcagag aaatcttcgc catccagatg
    69721 acgcacaccc ggccagccaa cgatgctgcg ggtccagata cgatcgtcat aagcgcctac
    69781 ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg cctgggaaac gagcgaactc
    69841 cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga atttacgcaa
    69901 ctccgggtaa ccatgcgcag gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc
    69961 ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa tcagaatgca
    70021 tttacccgcc gtcgctttga cgttgacctg agttggcgtc gggtgaccgt atttaccggt
    70081 ttcgcctgcg tccagaatgc tcatcacttt gaagttcatc tggccgattt ccattgaaca
    70141 ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga ttttatggta
    70201 ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca tataggccgc
    70261 cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt cgccaatcgc
    70321 cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc cgagatcgtc
    70381 gctcatcagt tgcaggtcag ccatcgggtt atcgacgcgg gcgttggcat ctacagccag
    70441 gcattgtgct ttcagcgcct cgcgcagggc aatcgcttca cgagcgtagc cgacaatacg
    70501 cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg caaagctgtc
    70561 tacatcgtgg ttgatgatgc cgtattcacg tgctttcact gcccaggccg aaagcccttg
    70621 cagcgtcgcg atgagtaaat cctgaaggtc agaggtttcc gccgttttac cacacatccc
    70681 ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac attgcacaca
    70741 aaacatgatc acacctttta aagttatatt taatatacat gtttaaggtt aagacgctta
    70801 acgcggggat aaaagagatt tttaatgcaa ctttaaggga gattgattta gcgcaatttt
    70861 ggcggcagag atctaccgcc agagaggtat tatgcagaga aaaaggcgat gagaatcggc
    70921 actaacagac taagaataaa accgtgaaca atcgccgccg ggaccatatc cagcccgcct
    70981 gtacgttgaa gaacgggcag ggtgaaatcc atcgacgtcg caccgcataa gcccagtgca
    71041 gtagagcggc tacggcgaat cagcccaggg attaacataa tagcaatcag ctcacgggcc
    71101 agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt caataaaata
    71161 ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt gatgggaaga
    71221 tcaagaataa aggcgttaat taaaccacca attaatgaac tggcaaccac caccacggcg
    71281 acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt attgcgcaac
    71341 tgaataccga cgaggaaaag tagcagaatt aaggtgtatt cactggcttc ggtcgcgtgt
    71401 tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac gccgcacagt
    71461 tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgatg atggtggttg
    71521 cgccatggca ggccgcgctc cagccacatc agggcggcaa tattacacag taaaataacg
    71581 gtaatactga cggcagaata atgcagaatt gccaacaagt tactggcgag gttatcgaga
    71641 aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccaact taatagctga
    71701 ttaataactc ttaatgcagc tttttggcga agcggaatga ggtaacccac aatcagggga
    71761 accagaatga ttaacagccc agaaaacatg aaaaaccagt ccttgcaaag aagattcgaa
    71821 atgcgcgatg acacactact gaaagcggaa ggacgagtaa agttgcaatt aaaaggaaat
    71881 gttatgcatc agagggggat ttttaattct atatgagtgt ttagcgttgt tgcgctcctt
    71941 tcatgtgcaa tgccatataa agccactggc gcagggagcg gccagtcgcc gccgctccct
    72001 gcccccccgg ccttcgggga acgcctcggc gattttaacg ccaccaacac ccgtgcggtt
    72061 tgtaacgtcg aagataaaac caccagggca gacaacatgc caatgctaat taatcgcgtt
    72121 tttccagtag agtccggtaa atcagaccac cgataatacc gccgacaatt ggcaccaccc
    72181 agaagaacca cagttgttct aatgcccagc cgccctggaa gatagcaacc gcagtactgc
    72241 gcgccgggtt aacggaagtg ttagtcaccg gaatactgat taagtgaatc agggttaagg
    72301 ccagaccaat ggcaattggc gcaaaaccag caggagcgaa tttgtcagtt gcgccgtgga
    72361 tcaccagcag gaaacctgca ctcaatacca gttcaactac cagcgcggaa agcatggaat
    72421 aaccgcctgg tgaatgctcg ccataaccgt tagaagcaaa accgctggct gccgcgtcaa
    72481 aacccgtttt accactggca attaaataca gcagcgccgc cgcaacaata ccgccgacaa
    72541 cctgggcaat tacgtagcca acgacttctt ttgccgggaa acgtccgcca gcccataaac
    72601 caatagtgac cgccgggtta aaatgaccac cagaaatatg accaacagca aaggccattg
    72661 tcagaacggt cagaccgaac gccaacgcca cgccggcaaa accaatgcct aattccggga
    72721 agcctgcggc cagtacggca ctaccacagc cgccaaaaac aagccagaaa gtaccaaaac
    72781 attcagctgc taattttctg aacatatcca cctcaattaa aaattgaccc tgtgaaaaat
    72841 atggtcgttt tatagggtcg tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt
    72901 aaaaacgaag gcaaatcatt cgccagataa ataaatccag taaatttgat ttagggcaac
    72961 agcgggttgc cccatatagt catttgtctg attgacagtg tagtgcaagc gaaagattta
    73021 atcctttagg cgtaataaaa aataatttat catgctaatt atttgatttt gttgtttttg
    73081 cagacttatc agcaagaggg agtataacgc gattattcgc tcatttttca gacatttgcc
    73141 atgcttaaat gtgatgtcat cacgtattag catggccttt cccgttatac tgccagcgta
    73201 aaggataagt cacatatttc tggaggggat atgattcttg agcgcgttga aattgtgggt
    73261 tttcgcggta tcaaccgttt gtcgttgatg ctggaacaaa acaacgtcct gattggggag
    73321 aacgcgtggg gtaaatccag cttgctggac gccttaaccc tgctgctatc gccagaatca
    73381 gatctctacc attttgagcg cgacgatttc tggttcccgc cgggagatat caacgggcga
    73441 gaacatcatc tgcatattat tttgaccttc cgcgaatcgc tgccaggccg acatcgggtt
    73501 cgccgctatc ggccgctgga agcgtgctgg acgccatgca ccgatggtta tcaccgtatt
    73561 ttttatcgtc tggaagggga gagtgcggaa gacggcagcg tgatgacact ccgcagtttt
    73621 ctcgataaag acggacatcc gattgatgtt gaggatatta acgatcaggc tcgtcatctg
    73681 gtgcgtttaa tgccggtgct gcgcttgcgt gatgcccgtt ttatgcgccg tattcgtaac
    73741 ggcacggtgc caaatgtccc caatgtggaa gtcaccgcgc gccagctcga tttcctcgcc
    73801 cgtgaattat cctcacatcc gcaaaatctc tctgatgggc agattcgtca gggactttcc
    73861 gcaatggtac agctgcttga gcattatttc tctgagcagg gggccggaca ggcgcgatat
    73921 cgtttaatgc ggcggcgagc cagcaatgag caacgaagct ggcgctatct ggatatcatc
    73981 aaccggatga ttgaccgacc tggagggcgc tcgtatcggg ttattttgct cggcctgttt
    74041 gctactttgt tgcaggcaaa aggcacattg cgactggata aagacgcccg tccattgttg
    74101 ctgatcgaag atccagaaac ccgtttacac cccattatgc tttcagttgc ctggcatctg
    74161 ttgaatcttc tgccattgca gcgcattgcc accaccaact ctggcgagct gctttcgtta
    74221 acgccggtag agcatgtttg ccgactggta cgtgagtcct cgcgcgttgc cgcctggcgt
    74281 ctggggccga gtggtttgag taccgaagat agccgacgca tctcttttca cattcgtttt
    74341 aatcgtccgt catcgctgtt tgctcgctgc tggctgttgg tggaagggga aacagaaacc
    74401 tgggttatca atgaactggc gcgtcagtgc ggacatcatt ttgatgccga agggatcaag
    74461 gtcattgagt ttgcccagtc cgggctaaag ccactggtta aatttgcccg gcgaatgggg
    74521 attgaatggc atgtactggt cgatggcgat gaagcaggga agaaatatgc cgctacggta
    74581 cgcagcctgt tgaataacga tcgggaagcc gaacgagaac atttaacggc gttaccggcg
    74641 ctggatatgg aacattttat gtatcgccag ggattttccg atgtgttcca ccgcgtggcg
    74701 caaatcccgg aaaatgtacc gatgaatctg cgcaaagtta tctcgaaagc gatccatcgc
    74761 tcttccaaac ccgatcttgc cattgaagtg gcaatggagg ccggacgtcg tggtgtggac
    74821 tccgtaccga cgctgctgaa aaaaatgttc tcacgcgtgc tgtggctggc gcgcggtcgg
    74881 gccgactaac tacaaaacat cgtggccatt tgtggctgaa tagcgtcgag catctcatag
    74941 cgccgacggt attcagcccg ttttttactg gcgatttcgg caatctcttt tcgtgctatc
    75001 tgtgctggaa ggcggtaatg gcgttcagca tcacatacgc cgccaaccga ctcccagaac
    75061 gcgttgtaat cggcatggat cttgccctct ttatcgcgat aacgcaggct gcggtaaata
    75121 tgcgtttcat tgctgacggc aataatctgc tctacctgca aacgttgggc aaacagacag
    75181 gccgcttcca tcacgaggcg tttgggaaat agcccgtggc aggctttcgt cgcattctgg
    75241 atttcctggt gtggaatttc ccattttgcg ccttgcagtc cgccaataaa catcgtcctt
    75301 ttcccctgat attcacacag ggtaaaagtg agctctgcca gaggaatacc ttcgctgttg
    75361 cggaacagga tggtgctgtc accttcttta tccattgaga tcatcatggt cagctcaagc
    75421 gtgaactgct cgccgttttt gccttccagc ttcgccagtt gcagcccggg ggtattcaaa
    75481 tataagctga attcttccgc cgacatacac ccgcggagta acgcataatg gtaacgtaac
    75541 gcctccagca attgcttacg gctaagattc gccgcaaggt aagggcgatg tagacgtaca
    75601 ggcagtcgcg gctggcgcgt taacaacaca ttgagattag gccagtggga aagttcgttc
    75661 atccactcaa cgcttaaacg cggcataatc aacgagcgca gcaaaaattt ctggcgaaaa
    75721 ctacggcgat gccagaattt acccggccga cactgtcctc gtgccagact aagaaaaagt
    75781 gacaggctgc tgagagattc agatggcgta aaggtccgtt cagttagctg cgacatattc
    75841 atgaaatcaa tggttataca tgacgtcgat ttaaccattg cgtatcttaa ccaaacatca
    75901 atagtgtgat tattaacgta aattttagtg ttttgttgat atttcgttga agttaatgac
    75961 ccggattggc atatggagta ttcagaatat ttatgaaaaa gcggaaaacc gtgaagaagc
    76021 gttacgttat tgcgatggtg atagtcatcg ccgggttgat tacgttatgg agaattctta
    76081 acgcacccgt gccgacttat cagacgctga ttgtgcgccc cggtgattta cagcaaagcg
    76141 tgctggcgac cggaaagctg gacgcgctgc gtaaggttga cgtgggcgcg caggtcagcg
    76201 gtcagttgaa aacgctgtcg gtggcgattg gcgataaagt aaaaaaagac cagcttttag
    76261 gggttattga tcctgaacag gctgaaaacc agatcaagga ggtggaagca acgctgatgg
    76321 agctacgcgc gcagcggcag caggcggaag cggagctgaa actggcgcgg gtgacgtatt
    76381 cccgtcagca acgtcttgca caaacgcaag cggtttcaca gcaggatctc gataacgccg
    76441 cgacggagat ggctgtgaaa caggcgcaaa ttggcaccat tgacgcgcaa atcaagcgca
    76501 atcaggcctc tctcgatacg gctaaaacca atctcgatta cacccgcatc gttgccccga
    76561 tggccgggga agtcacgcaa atcaccactc tgcaaggcca gacggtgatt gccgcacaac
    76621 aagcaccgaa cattctgacg ctggcagata tgagcaccat gctggtaaaa gcgcaggttt
    76681 ctgaagcgga tgtaatccac ctgaagccgg ggcaaaaagc ctggtttacg gtgcttggcg
    76741 atccactgac gcgctacgag gggcaaatca aggatgtgct accgacgccg gaaaagatta
    76801 acgacgctat tttctattac gcccgttttg aagtacccaa ccccaatggt ttactgcggc
    76861 tggatatgac cgcgcaagtg catattcagc tcaccgatgt gaaaaatgtg ctgacgatcc
    76921 ctctgtcggc gttaggcgat ccggttggcg ataatcgtta taaagtcaaa ttgttgcgta
    76981 atggtgaaac acgcgagcgt gaagtgacga ttggcgcacg taacgatacc gatgttgaga
    77041 ttgtcaaagg gctggaagcg ggcgatgaag tggtgattgg tgaggccaaa ccaggagctg
    77101 cgcaatgacg cccttgctcg aattaaagga tattcgtcgc agctatcctg ccggtgatga
    77161 gcaggttgag gtgctgaagg gcatcagcct cgatatttat gccggtgaga tggtagcgat
    77221 tgtcggcgct tcgggttccg gtaaatcgac cctgatgaat attctcggct gcctggataa
    77281 ggccaccagc ggcacctatc gcgtcgccgg tcaggatgtt gccacgctgg acgccgatgc
    77341 gctggcgcaa ctgcgccgcg agcatttcgg ctttattttc cagcgttacc atttgctttc
    77401 gcatttaacc gccgagcaga acgttgaagt acccgccgtc tatgccggtc ttgagcggaa
    77461 acagcggctg cttcgcgccc aggagttact gcaacgactg gggctggaag accgtacaga
    77521 gtattacccg gcacagcttt cgggtggcca gcaacagcgc gtcagtatcg cgcgggcatt
    77581 gatgaacggc ggtcaggtaa ttcttgccga tgaaccgacc ggcgcactgg acagccattc
    77641 tggcgaagag gtgatggcga tcctgcatca gctgcgcgat cgcgggcata cggtgattat
    77701 cgtcacccac gatccgcagg ttgctgctca ggccgagcgg gtgatcgaaa ttcgcgacgg
    77761 cgaaattgtg cgcaatcctc ccgccgttga aaaagtgaat gctactggcg gaacggaacc
    77821 tgttgtcaac acggcgtctg gctggcggca gtttgtcagc ggttttaacg aggcgctgac
    77881 gatggcatgg cgggcgctgg cagcgaataa aatgcgtact ttactgacca tgctggggat
    77941 tattatcggt attgcgtcgg tggtttccat tgtcgtggtg ggcgacgccg ccaaacaaat
    78001 ggtgctggcg gatattcgtt ctattggtac gaatattatt gatgtctatc ccgggaaaga
    78061 ttttggcgat gacgatccgc aatatcagca ggcgctgaag tacgacgact taatcgccat
    78121 ccaaaaacaa ccgtgggttg cctctgccac gcccgccgtc tcgcaaaacc tgcgcctgcg
    78181 ttataacaat gttgatgtgg ctgccagtgc caatggtgtg agcggcgatt attttaatgt
    78241 ctatggcatg accttcagtg aagggaacac ctttaatcag gagcagctga acggtcgtgc
    78301 gcaggtcgtg gttctcgaca gtaatactcg ccgccagctt ttcccccata aagcagatgt
    78361 ggttggcgag gtgattctgg tcggcaatat gcccgccaga gtcattggtg tggcggaaga
    78421 aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctacctt acagcacgat
    78481 gtccgggcga gttatgggcc agtcgtggct gaactccatt actgtcaggg tgaaagaagg
    78541 atttgacagc gctgaggcgg aacagcaact cacgcgttta ctttcactgc gccacggtaa
    78601 gaaggatttc tttacctgga acatggatgg cgtcttgaaa accgttgaaa agaccacacg
    78661 tactttacaa ctgtttctga cgctggttgc ggtgatttcg ctggtggtgg gcggtattgg
    78721 tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg gcattcgcat
    78781 ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttt ttgatcgaag ccgtactggt
    78841 ttgcctggtt ggtggcgcgt tgggaataac actgtcactg ttaattgctt tcaccttgca
    78901 gcttttctta cccggctggg agattggttt ttcaccgttg gcactgctgc tggcgtttct
    78961 ctgctcgacg gtcaccggga ttttatttgg ctggttaccc gcacgaaatg cggcacgact
    79021 ggatccagta gatgctctgg cacgagagta atttttgaga taaaaatgcc agccgatcgg
    79081 gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctacgcg actgccgctt
    79141 ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg tggacatcaa
    79201 actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg gtggaataat
    79261 gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct ttggcattgt
    79321 tgaaccactt gacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt
    79381 aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa ctctagaaat
    79441 acaatttaga gaattagggc gagccgtcaa gcatttgaca ggggccaggg ggcaggtatg
    79501 aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt cgctgatgcc
    79561 aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat aagctcttgt
    79621 cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca atgggtaaaa
    79681 cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac
    79741 cgccatctat ttataaagtg atattagtca atgatgatta tactccgatg gagtttgtta
    79801 ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg
    79861 ctgttcacta ccaggggaag gccatttgcg gagtctttac cgctgaggtt gcagaaacca
    79921 aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg tgtacgctag
    79981 aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat caagaactgg
    80041 aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg
    80101 tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg ctggaagcgt
    80161 gttctgtgga tttggttgcg ctccgtcagg aactggaagc ctttattgaa caaaccacac
    80221 ccgttctgcc tgccagtgaa gaggagcgcg acacacaacc gacgctgagt tttcagcgtg
    80281 tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt accggtgcaa
    80341 acgttctggt cgctatcttt agcgaacagg agtcgcaggc ggcatatctg ttgcgcaaac
    80401 atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt aaagacgagc
    80461 cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct ggtggggagg
    80521 aacgtatgga gaatttcacg acgaacctga atcagcttgc gcgagtgggc ggaatcgacc
    80581 cactgattgg tcgtgagaag gagctggagc gtgctattca ggttctctgc cgtcgccgta
    80641 aaaacaaccc gctgctggtg ggggagtctg gtgtcggtaa aaccgcgatt gcggaggggc
    80701 ttgcatggcg aattgttcag ggcgatgtgc cggaagtgat ggctgactgt acgctttact
    80761 ctctcgatat cggttccctg ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt
    80821 ttaaagcgtt gctcaagcag ctggagcagg acactaacag catcctgttt attgatgaga
    80881 tccacaccat tatcggtgcg ggtgcagcgt ctggtggcca ggtcgatgcg gctaacctga
    80941 ttaaaccgtt gctctccagc ggtaaaatcc gcgtaattgg ttcgacaacc tatcaggagt
    81001 tcagcaacat ttttgagaaa gaccgtgctc tggcgcgtcg cttccagaaa attgatatta
    81061 ctgaaccgtc aatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag
    81121 cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga
    81181 aatacattaa cgatcgtcat ctgccggata aagcgattga tgttatcgac gaagcgggcg
    81241 ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg
    81301 aatccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag agtgaccgcg
    81361 ataccctgaa aaacctcggc gatcgcctga aaatgctggt cttcggtcag gataaagcca
    81421 ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac gaacataaac
    81481 cggtcggttc gttcctgttt gccggcccta ctggggtcgg gaaaacagag gtgacggtac
    81541 agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag tatatggaac
    81601 gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt gatcagggcg
    81661 gtttgctgac tgatgcggtc atcaagcatc cacatgcggt gctgctgctg gacgaaatcg
    81721 agaaagcgca cccggacgtg ttcaacatcc tcttgcaggt gatggacaac ggtacactga
    81781 ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg accactaacg
    81841 cgggggtacg tgaaaccgag cgcaaatcca ttggtcttat ccaccaggat aacagcaccg
    81901 atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt ctcgacaaca
    81961 ttatctggtt cgatcatctg tcaaccgacg tgatccatca ggtggtggat aaattcatcg
    82021 tcgagttgca ggttcagttg gatcagaaag gtgtttctct ggaagtgagc caggaagcgc
    82081 gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg
    82141 tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg
    82201 acggcggtca ggtcaccgtc gcgctggata aagagaaaaa tgagctgact tacggattcc
    82261 agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtctg gtaggttggt
    82321 gaagtccgta atctcgtcag gggttacgga ctttttattt atggggggag gaggttcaga
    82381 cccttttttt aatgatgatg ctaagttatt gataattagt gctgcgggta ggtaaggata
    82441 aaaaagggtg gcagcaggag attgagatgg ttttgcttta ttaacaacgg gctaaacgtg
    82501 tagtatttga gttcactgcc gtacaggcag cttagaaagc acacgtcgag ctggtgggga
    82561 ttaatgctgg gttcactgcc gtacaggcag cttagaaatt gccgcggatc ctgtctgcca
    82621 ataatgacaa gttcactgcc gtacaggcag ataaaatgcg aaaaaaaacc cgtactttcg
    82681 tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg
    82741 tatacacact ttccaggcgt gctccttcag ccactcgg
//
LOCUS       DAZFRE010000007        82283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFRE010000007 DAZFRE010000000
VERSION     DAZFRE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.007"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..593)
                     /locus_tag="ACHKUI_000570"
     CDS             complement(<1..593)
                     /locus_tag="ACHKUI_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6596834.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKUI_000571"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKUI_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6596835.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKUI_000572"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKUI_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6596836.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKUI_000573"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKUI_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6596837.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKUI_000574"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKUI_000574"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6596838.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(4236..4595)
                     /gene="tusC"
                     /locus_tag="ACHKUI_000575"
     CDS             complement(4236..4595)
                     /gene="tusC"
                     /locus_tag="ACHKUI_000575"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6596839.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4595..4981)
                     /gene="tusD"
                     /locus_tag="ACHKUI_000576"
     CDS             complement(4595..4981)
                     /gene="tusD"
                     /locus_tag="ACHKUI_000576"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6596840.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4981..5703)
                     /gene="yheO"
                     /locus_tag="ACHKUI_000577"
     CDS             complement(4981..5703)
                     /gene="yheO"
                     /locus_tag="ACHKUI_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6596841.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5870..6682)
                     /gene="fkpA"
                     /locus_tag="ACHKUI_000578"
     CDS             complement(5870..6682)
                     /gene="fkpA"
                     /locus_tag="ACHKUI_000578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6596842.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKQEADAKAADSAKK"
     gene            6903..7121
                     /gene="slyX"
                     /locus_tag="ACHKUI_000579"
     CDS             6903..7121
                     /gene="slyX"
                     /locus_tag="ACHKUI_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6596843.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7170..7760)
                     /gene="slyD"
                     /locus_tag="ACHKUI_000580"
     CDS             complement(7170..7760)
                     /gene="slyD"
                     /locus_tag="ACHKUI_000580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6596844.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7855..8055)
                     /gene="yheV"
                     /locus_tag="ACHKUI_000581"
     CDS             complement(7855..8055)
                     /gene="yheV"
                     /locus_tag="ACHKUI_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6596845.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8065..9870)
                     /gene="kefB"
                     /locus_tag="ACHKUI_000582"
     CDS             complement(8065..9870)
                     /gene="kefB"
                     /locus_tag="ACHKUI_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6596846.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNHSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9870..10421)
                     /gene="kefG"
                     /locus_tag="ACHKUI_000583"
     CDS             complement(9870..10421)
                     /gene="kefG"
                     /locus_tag="ACHKUI_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6596847.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10552..12465
                     /gene="yheS"
                     /locus_tag="ACHKUI_000584"
     CDS             10552..12465
                     /gene="yheS"
                     /locus_tag="ACHKUI_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6596848.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12465..13487
                     /gene="yheT"
                     /locus_tag="ACHKUI_000585"
     CDS             12465..13487
                     /gene="yheT"
                     /locus_tag="ACHKUI_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6596849.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13481..13699
                     /gene="yheU"
                     /locus_tag="ACHKUI_000586"
     CDS             13481..13699
                     /gene="yheU"
                     /locus_tag="ACHKUI_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6596850.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13753..14622
                     /gene="prkB"
                     /locus_tag="ACHKUI_000587"
     CDS             13753..14622
                     /gene="prkB"
                     /locus_tag="ACHKUI_000587"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6596851.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14677..15081)
                     /gene="yhfA"
                     /locus_tag="ACHKUI_000588"
     CDS             complement(14677..15081)
                     /gene="yhfA"
                     /locus_tag="ACHKUI_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6596852.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15383..16015
                     /gene="crp"
                     /locus_tag="ACHKUI_000589"
     CDS             15383..16015
                     /gene="crp"
                     /locus_tag="ACHKUI_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6596853.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16066..18156
                     /gene="yhfK"
                     /locus_tag="ACHKUI_000590"
     CDS             16066..18156
                     /gene="yhfK"
                     /locus_tag="ACHKUI_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6596854.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18234..19454)
                     /gene="argD"
                     /locus_tag="ACHKUI_000591"
                     /gene_synonym="dapC"
     CDS             complement(18234..19454)
                     /gene="argD"
                     /locus_tag="ACHKUI_000591"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6596855.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19540..20103)
                     /gene="pabA"
                     /locus_tag="ACHKUI_000592"
     CDS             complement(19540..20103)
                     /gene="pabA"
                     /locus_tag="ACHKUI_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6596856.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20135..20737)
                     /gene="fic"
                     /locus_tag="ACHKUI_000593"
     CDS             complement(20135..20737)
                     /gene="fic"
                     /locus_tag="ACHKUI_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6596857.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20727..20894)
                     /gene="yhfG"
                     /locus_tag="ACHKUI_000594"
     CDS             complement(20727..20894)
                     /gene="yhfG"
                     /locus_tag="ACHKUI_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6596858.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20999..21571)
                     /gene="ppiA"
                     /locus_tag="ACHKUI_000595"
                     /gene_synonym="rotA"
     CDS             complement(20999..21571)
                     /gene="ppiA"
                     /locus_tag="ACHKUI_000595"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6596859.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21842..23023
                     /gene="tsgA"
                     /locus_tag="ACHKUI_000596"
     CDS             21842..23023
                     /gene="tsgA"
                     /locus_tag="ACHKUI_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6596860.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23285..25828
                     /gene="nirB"
                     /locus_tag="ACHKUI_000597"
     CDS             23285..25828
                     /gene="nirB"
                     /locus_tag="ACHKUI_000597"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6596861.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25825..26151
                     /gene="nirD"
                     /locus_tag="ACHKUI_000598"
     CDS             25825..26151
                     /gene="nirD"
                     /locus_tag="ACHKUI_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6596862.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            26277..27083
                     /gene="nirC"
                     /locus_tag="ACHKUI_000599"
     CDS             26277..27083
                     /gene="nirC"
                     /locus_tag="ACHKUI_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6596863.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27102..28475
                     /gene="cysG"
                     /locus_tag="ACHKUI_000600"
     CDS             27102..28475
                     /gene="cysG"
                     /locus_tag="ACHKUI_000600"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6596864.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28727..28894
                     /gene="yhfL"
                     /locus_tag="ACHKUI_000601"
     CDS             28727..28894
                     /gene="yhfL"
                     /locus_tag="ACHKUI_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6596865.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(29002..30087)
                     /gene="yhfS"
                     /locus_tag="ACHKUI_000602"
     CDS             complement(29002..30087)
                     /gene="yhfS"
                     /locus_tag="ACHKUI_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6596866.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAVRVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(30099..31403)
                     /gene="yhfT"
                     /locus_tag="ACHKUI_000603"
     CDS             complement(30099..31403)
                     /gene="yhfT"
                     /locus_tag="ACHKUI_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO6596867.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(31415..31768)
                     /gene="yhfU"
                     /locus_tag="ACHKUI_000604"
     CDS             complement(31415..31768)
                     /gene="yhfU"
                     /locus_tag="ACHKUI_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO6596868.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(31779..32657)
                     /gene="php"
                     /locus_tag="ACHKUI_000605"
     CDS             complement(31779..32657)
                     /gene="php"
                     /locus_tag="ACHKUI_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO6596869.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSAQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(32654..33880)
                     /gene="yhfW"
                     /locus_tag="ACHKUI_000606"
     CDS             complement(32654..33880)
                     /gene="yhfW"
                     /locus_tag="ACHKUI_000606"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6596870.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLPDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDDKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(33881..35044)
                     /gene="yhfX"
                     /locus_tag="ACHKUI_000607"
     CDS             complement(33881..35044)
                     /gene="yhfX"
                     /locus_tag="ACHKUI_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO6596871.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVEAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            complement(35128..35490)
                     /gene="yhfY"
                     /locus_tag="ACHKUI_000608"
     CDS             complement(35128..35490)
                     /gene="yhfY"
                     /locus_tag="ACHKUI_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6596872.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(35507..36412)
                     /gene="yhfZ"
                     /locus_tag="ACHKUI_000609"
     CDS             complement(35507..36412)
                     /gene="yhfZ"
                     /locus_tag="ACHKUI_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO6596873.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(36572..37576)
                     /gene="trpS"
                     /locus_tag="ACHKUI_000610"
     CDS             complement(36572..37576)
                     /gene="trpS"
                     /locus_tag="ACHKUI_000610"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6596874.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(37569..38327)
                     /gene="gph"
                     /locus_tag="ACHKUI_000611"
     CDS             complement(37569..38327)
                     /gene="gph"
                     /locus_tag="ACHKUI_000611"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6596875.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSLNDLLPALGLPHSENQESKND"
     gene            complement(38320..38997)
                     /gene="rpe"
                     /locus_tag="ACHKUI_000612"
     CDS             complement(38320..38997)
                     /gene="rpe"
                     /locus_tag="ACHKUI_000612"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6596876.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(39015..39851)
                     /gene="dam"
                     /locus_tag="ACHKUI_000613"
     CDS             complement(39015..39851)
                     /gene="dam"
                     /locus_tag="ACHKUI_000613"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6596877.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(39958..41244)
                     /gene="damX"
                     /locus_tag="ACHKUI_000614"
     CDS             complement(39958..41244)
                     /gene="damX"
                     /locus_tag="ACHKUI_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6596878.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEELDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQIPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(41336..42424)
                     /gene="aroB"
                     /locus_tag="ACHKUI_000615"
     CDS             complement(41336..42424)
                     /gene="aroB"
                     /locus_tag="ACHKUI_000615"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6596879.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLNTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(42481..43002)
                     /gene="aroK"
                     /locus_tag="ACHKUI_000616"
     CDS             complement(42481..43002)
                     /gene="aroK"
                     /locus_tag="ACHKUI_000616"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6596880.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(43403..44641)
                     /gene="hofQ"
                     /locus_tag="ACHKUI_000617"
     CDS             complement(43403..44641)
                     /gene="hofQ"
                     /locus_tag="ACHKUI_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO6596881.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(44553..44957)
                     /gene="hofP"
                     /locus_tag="ACHKUI_000618"
     CDS             complement(44553..44957)
                     /gene="hofP"
                     /locus_tag="ACHKUI_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6596882.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(44947..45387)
                     /gene="hofO"
                     /locus_tag="ACHKUI_000619"
     CDS             complement(44947..45387)
                     /gene="hofO"
                     /locus_tag="ACHKUI_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6596883.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            complement(45371..45910)
                     /gene="hofN"
                     /locus_tag="ACHKUI_000620"
     CDS             complement(45371..45910)
                     /gene="hofN"
                     /locus_tag="ACHKUI_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6596884.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAIGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(45910..46689)
                     /gene="hofM"
                     /locus_tag="ACHKUI_000621"
     CDS             complement(45910..46689)
                     /gene="hofM"
                     /locus_tag="ACHKUI_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6596885.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            46809..49361
                     /gene="mrcA"
                     /locus_tag="ACHKUI_000622"
     CDS             46809..49361
                     /gene="mrcA"
                     /locus_tag="ACHKUI_000622"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6596886.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(49535..50095)
                     /gene="nudE"
                     /locus_tag="ACHKUI_000623"
     CDS             complement(49535..50095)
                     /gene="nudE"
                     /locus_tag="ACHKUI_000623"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6596887.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            50415..52550
                     /gene="igaA"
                     /locus_tag="ACHKUI_000624"
     CDS             50415..52550
                     /gene="igaA"
                     /locus_tag="ACHKUI_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6596888.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFVDAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVTTSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            52615..53283
                     /gene="yrfG"
                     /locus_tag="ACHKUI_000625"
     CDS             52615..53283
                     /gene="yrfG"
                     /locus_tag="ACHKUI_000625"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6596889.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            53294..53695
                     /gene="hslR"
                     /locus_tag="ACHKUI_000626"
     CDS             53294..53695
                     /gene="hslR"
                     /locus_tag="ACHKUI_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6596890.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            53720..54598
                     /gene="hslO"
                     /locus_tag="ACHKUI_000627"
     CDS             53720..54598
                     /gene="hslO"
                     /locus_tag="ACHKUI_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6596891.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(54661..56385)
                     /gene="yhgE"
                     /locus_tag="ACHKUI_000628"
     CDS             complement(54661..56385)
                     /gene="yhgE"
                     /locus_tag="ACHKUI_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6596892.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PVTVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLATALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWMPVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRQITKEKLLTAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            56764..58386
                     /gene="pckA"
                     /locus_tag="ACHKUI_000629"
     CDS             56764..58386
                     /gene="pckA"
                     /locus_tag="ACHKUI_000629"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6596893.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(58463..59815)
                     /gene="envZ"
                     /locus_tag="ACHKUI_000630"
     CDS             complement(58463..59815)
                     /gene="envZ"
                     /locus_tag="ACHKUI_000630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6596894.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(59812..60531)
                     /gene="ompR"
                     /locus_tag="ACHKUI_000631"
     CDS             complement(59812..60531)
                     /gene="ompR"
                     /locus_tag="ACHKUI_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6596895.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            60759..61235
                     /gene="greB"
                     /locus_tag="ACHKUI_000632"
     CDS             60759..61235
                     /gene="greB"
                     /locus_tag="ACHKUI_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6596896.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            61332..63653
                     /gene="yhgF"
                     /locus_tag="ACHKUI_000633"
     CDS             61332..63653
                     /gene="yhgF"
                     /locus_tag="ACHKUI_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6596897.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            64109..64336
                     /gene="feoA"
                     /locus_tag="ACHKUI_000634"
     CDS             64109..64336
                     /gene="feoA"
                     /locus_tag="ACHKUI_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6596898.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            64353..66674
                     /gene="feoB"
                     /locus_tag="ACHKUI_000635"
     CDS             64353..66674
                     /gene="feoB"
                     /locus_tag="ACHKUI_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6596899.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCISAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            66674..66910
                     /gene="feoC"
                     /locus_tag="ACHKUI_000636"
     CDS             66674..66910
                     /gene="feoC"
                     /locus_tag="ACHKUI_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6596900.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            67113..68006
                     /gene="rpnA"
                     /locus_tag="ACHKUI_000637"
     CDS             67113..68006
                     /gene="rpnA"
                     /locus_tag="ACHKUI_000637"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6596901.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKENLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            68078..68197
                     /locus_tag="ACHKUI_000638"
                     /pseudo
     CDS             68078..68197
                     /locus_tag="ACHKUI_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(68226..68996)
                     /gene="bioH"
                     /locus_tag="ACHKUI_000639"
     CDS             complement(68226..68996)
                     /gene="bioH"
                     /locus_tag="ACHKUI_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6596902.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFHHLLVALKQRV"
     gene            69034..69717
                     /gene="gntX"
                     /locus_tag="ACHKUI_000640"
     CDS             69034..69717
                     /gene="gntX"
                     /locus_tag="ACHKUI_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6596903.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            69776..70351
                     /gene="nfuA"
                     /locus_tag="ACHKUI_000641"
     CDS             69776..70351
                     /gene="nfuA"
                     /locus_tag="ACHKUI_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6596904.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            70715..72031
                     /gene="gntT"
                     /locus_tag="ACHKUI_000642"
     CDS             70715..72031
                     /gene="gntT"
                     /locus_tag="ACHKUI_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6596905.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(72142..74226)
                     /gene="malQ"
                     /locus_tag="ACHKUI_000643"
     CDS             complement(72142..74226)
                     /gene="malQ"
                     /locus_tag="ACHKUI_000643"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6596906.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWSEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(74236..76629)
                     /gene="malP"
                     /locus_tag="ACHKUI_000644"
     CDS             complement(74236..76629)
                     /gene="malP"
                     /locus_tag="ACHKUI_000644"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6596907.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            77252..79957
                     /gene="malT"
                     /locus_tag="ACHKUI_000645"
     CDS             77252..79957
                     /gene="malT"
                     /locus_tag="ACHKUI_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6596908.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(80000..81016)
                     /gene="rtcA"
                     /locus_tag="ACHKUI_000646"
     CDS             complement(80000..81016)
                     /gene="rtcA"
                     /locus_tag="ACHKUI_000646"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6596909.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITELFFVVGEKRVSAEVVAAQLVQEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(81020..82246)
                     /gene="rtcB"
                     /locus_tag="ACHKUI_000647"
     CDS             complement(81020..82246)
                     /gene="rtcB"
                     /locus_tag="ACHKUI_000647"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6596910.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        19773 a        22436 c        20666 g        19408 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatagtgt atgatgcgcg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttcagga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gattgcagct gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtacacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgctcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     2041 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatacg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccaccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tggaaatgat gtcgttcgaa
     4021 attcgaccag tcaaaccgcg ggcaataagg tcttcgttca gagcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggttg ccgtcaactg cggcagttac gccatcttgc
     4141 aataatagca gttcgtcacc ttcactgagc agacgcagca gcgcagcaaa gtccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata acgcctcaaa acctcaaaat aacatcgtag
     4261 ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg tggcttcgac cacaaagggt
     4321 gtctgcggat ctaacccacg ttcgcgcagt gaagccgcgc aaatccagca ctgttcaata
     4381 tcgtacagac ccaacaattt aaaagtggca atgtaatcac gcgccagcac tgcatcgggc
     4441 ttttgtcctg gcagcagctg aaaaacgcca tcagcaataa agaagacggc cagatcgtca
     4501 gttaatgcgg aagttgccag taaagcatct aaaccttccc ggcctgcagt tgtaccatga
     4561 ggcgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg
     4621 tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg
     4681 acgaagcgag ccccagtctt ccggcctccg tttcatcaac aacgccacgg cgtaatgctg
     4741 ccgctacgca gatattcagc gccacaccat gttgcgcatt caactgttgc cagccgcgca
     4801 caaggtcaaa ttcgtcgctt gccggtgagg tcaattggtt agcgttatag accccttccc
     4861 gatagaaaaa aacgctgctt aactcatggc cttctgctat cagcgcctgc gcaaactgaa
     4921 aagcactact cgcctgttgc gtaccgtatg ctggcccggt caccacgatg gcaaaacgca
     4981 ttacttatct tgcccctgga aatcaccgct cttgaactgg cggatgtaga gatagacagt
     5041 gtgtttggag atgttcaggc ggtcagcaac ctggttgatc gcgtctttaa tatcgaagat
     5101 ccctttctcg tagagattta gcacgatctg acgatttttg gcgttattag aaacattgcg
     5161 atcggcattc acttcttcga tggtgaactc cagcgtttgg gtaaccagat cttcaacaga
     5221 agaggcaaag ttgacgcttg aaccgacatc cggagtttcc ggcggcacaa aagtgctcat
     5281 aatctgcgag aagggaacat caagattcat attgatgcac agcagaccaa ttacacgctg
     5341 ttcgcggtta cgaatcgcga tagtcaggga cttcattaat acgccgcttt tggcgcgagt
     5401 aaagtagcat ttagaaacgc tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc
     5461 caggtcagta attggcgaac caatcttccg gcctgtatgt tcaccgttag caatgcgaat
     5521 ggctgaacat tttagatcct gcaaagagtg caaaacgatt tcacagtggg agccaataag
     5581 catcgctaac ccgtccacca ccgcttcgta ggatttcaga atatcaaaat cggtctggtc
     5641 aaaaggacgt tgatccagta aatccaactc actggtttcg ttggttaaaa gcgacctgga
     5701 catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac ttttcagggc aggctcatta
     5761 atgatgcggg taactaaatt aatacagcgg aggttccgct ttccagcact aattatatcc
     5821 ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcggt tcttaatgct tattttttag
     5881 cagagtctgc ggctttcgca tcagcttcct gctttgcatc agccttcggc gctggtttca
     5941 catccagcag ctctacgtca aacaccaggg tagaattcgg tgggatcccc ggaacacccg
     6001 ctttgccgta agccagttct ggtggaataa ccagtttgat cttaccgcct ttcttgatgt
     6061 tcttcagacc ttctgtccaa cccgggataa caccgtccag acggaaagag agcggttcac
     6121 cacgggtgta agagttgtcg aactctttac cgtcgatcag cgtacctttg tagttcacta
     6181 caacagtatc gctgtctttc ggtgcttcgc ctttaccggc ttctactacc tgataaacca
     6241 gaccagtgga agaggttttc acacctttct ctttggcaaa tttctcgcgg tactctttac
     6301 cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc ctgagcagaa gacttcacac
     6361 gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc ggaaagtttg ctcttatcag
     6421 caaatgcatc ctgaacacca gcgatcagct gatctttatc cagtttgatg cccagttttt
     6481 cttgttcttt aagagagttt tccatgtaac gacccagcga agcacccagt gcataagctg
     6541 atttctgatc gtcatttttg aacgctgctt tgctgtcagc agttgtagca ggttttgcag
     6601 cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc cattgtggtc gccagcagcg
     6661 ttactttaaa cagtgatttc atccatatct ccaggatcgg ggcatctcac cccagggtta
     6721 actattatca gaagggtact ataaagcgtt gtcgaacaaa tctacataca gacacgccct
     6781 attatcatct attttcagac tctttttgtt taaattagtt tcgatgaccg cgaaatgagt
     6841 gctgtctcgg gcagcaaagt taagtagaat ccgcggcgac cattcgacaa aagaggtgaa
     6901 tcatgcagga tttatcattg gaagcacgcc tggcagagct ggagagccga ttggcttttc
     6961 aggaaatcac cattgaagaa ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga
     7021 aactgcgcga tcatctgcgt ctgctgaccg agaagctaaa agccagccag ccgtcgaaca
     7081 tcgcgtcgca ggctgaagaa acgccaccgc cacattattg aggcgtaaaa aaagcgggga
     7141 ttccccgctt ttttgtcacc ttttcggtat tagtggcaac cgcaaccgcc gttgccttta
     7201 ccgccacagc agccttcgcc accgtgttca tgaccgtgat cgtggccatg accaccgcag
     7261 caaccgtcgt ggtcgtgatc gtggtggtga tcgtgcgcgc cgtgaacgtg accatgagcc
     7321 agttcttctt cggtcgcttc gcgaatcgcc acaacttcaa cgttgaattt caggttctga
     7381 ccagccagca tgtggttacc atcaaccact acgtgatcgt cttcaaccgc agtgatttca
     7441 accggtaccg gaccctggtc ggtttcagcc aggaaacgca tacctacctg cagttcatca
     7501 acgcccataa atacgtcttt aggaacgcgt tgcaccaggt tttcgtcgta ctgaccgtaa
     7561 gcgtcgttcg cgccaacagc gacatcaaat ttgtcgccaa cttcatgacc ttccagcgct
     7621 gtttccaggc cagagatcag ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc
     7681 ggagactcat caaccaacac accgtcttct gtacgtacct gataggccag gctgaccacc
     7741 aggtcttttg ctactttcat gatatctcct gagcatggga agaatagtgg cgcagattgt
     7801 agcggaattc tgcagccgtg tactcactag cttaaaaaaa ctcggcgcat atcgctagtc
     7861 cggatgaaaa atcccgatca cttgctcatc tttgcggacg tgatcgcggg cttctttgtc
     7921 tgcttctcgc atctgatgtc cacacttaac acattctacg atatcaatat tattttcgcg
     7981 ccacatcgcc attgaatcct gtgcctgaca ggccgggcat tttgcgcccg caataaaacg
     8041 ttttcggatt gccatcttta ccctctactc aaattcatcc cagccgtcca gctggcgtcg
     8101 ttcttgttgc atttcacgct ggaaaatctc ttccagttca cgtcgggctt ccctggcgcg
     8161 agaaatttgt acggtatcag catgcatcgg gattaactcc cgcagcattc gcatatccag
     8221 gcggcgaaaa tgcagttgcg cgcgctgcgc ctgatgcgga tgcatgccaa gcgtgaccag
     8281 cgtcttgcgc cccagctcta acgcactgga gaatgtttca cgggaaaact gcgtcacccc
     8341 tgcctgtaat aactcatgcg cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg
     8401 cggaaagtgc tgctggcata tttccaccag cttcatagtg tcttccggct cgttacaggt
     8461 aatgacgata gactcagcgg cctctgcacc cgcagaacgt aaaagatcta cctgcgtggc
     8521 gtcgccgtaa taaactttgt agccatattt gcgcatcagg ttaacggcac tgatatcccg
     8581 ctccagtaca gtaatgcgca ttttatttgc catcagcaaa cgaccaatca cctgaccaaa
     8641 acgcccaaag cccacgacaa tgacctgcgg tttatcatcg ttgacccatg gtttttcgtc
     8701 ttcttcttcc ggtccgttaa actggcggga tagccattta tcgaccagct tcatcagcag
     8761 cggcgtggtc atcatggaaa gcgtcaccgt caccagcaac agcgccatct ggtcgccctg
     8821 gaataagcgt tgtgaagaag cggtagaaaa gaggacaaag gcaaactcac cgccctgact
     8881 caacacgcca gcaaactgca tccgctcaga actgcgcacg ccatacaatc gcgccagcag
     8941 atacagcacg agaattttca ccgccaccag cacaaccaca cttatcacta cccacaacag
     9001 atgggtataa agcaccccga ggttgagcga catgccgaca gagataaaga acaaaccaag
     9061 cagcaagcct ttgaagggat cgatagccgt ttccagttca tggcgatatt cactttccgc
     9121 cagtagcaca cccgcaataa acgtaccgag cgccatcgac aaccccagcg catccataaa
     9181 caatgcggaa cccaacacca gcagcagcgt cgcggcggtg aacacttccc gcacaccaga
     9241 agctgcaata aaacggaata ccggacgcag taaatagcgc ccgccaatca gcatgccgac
     9301 aaacgccagc accttcatgc cgatcttcat ccagtcgaaa tgttcgtctg ccgaacccgc
     9361 caacaacggc actaacgcca gtgccgggat caccgccaga tcctgaaaca gcagtaccga
     9421 gaaacctaac tggccggatt cgctgtgatt catccctttc tcacgcatca attgcaacgc
     9481 cattgcagtt gaagacatcg caaggccaat gccaccgacc accgccgcct gccatgagaa
     9541 atgggtcagc atcaataatc ctgccagcaa cgccgcgctt aacagcacct gtgccgcgcc
     9601 tacgccaaaa atcgaacgcc gcagttgcca aagcttggag ggattcaact caaggccgat
     9661 gataaacatc aggaatacca cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct
     9721 aataaacccc agcccccacg ggccaatcgc aatccccgcc agcaaatatc ccaacactgc
     9781 accaataccc agccgcgatg ccagcggcac cgcagccacc gccgcgaaga gaaacagcac
     9841 tcctgcgagt aaaaaatcag aaccttccat cagcagcctc ctggagacag cggatttgcc
     9901 agccagtcac cgtaggctct ggcgtggctc gccagctcct gtgcgctttg ccgtctcgcc
     9961 cagtaaataa tgatggggct taaccaatgc atccggcaca tgcccgccgc cagttcaaag
    10021 gggcgcagaa catcgctcat cgggtagcga ttcaacgcgt cataacggta agcactttcc
    10081 ggctccccgg tggtaatcac gctacgccag tactttcccg ccagttggtt tccccccggt
    10141 ccgctggcaa aaccacggct taatacccgg tccagccact ctttcagtag cgccgggcag
    10201 ctataggtat aaagaggatg ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc
    10261 tcacgaggga tatcaataaa aaagtcggga tagtgcgcgt aaaggtcgtg cacggtaaca
    10321 ttgctgagct gcgtggccgg tttaagcaga acccggtttg ccaccgagtc ctgagattcc
    10381 ggatgggcat acagcagcaa aactttcgct ggctgagaca tcatccccct cccggtatgg
    10441 tttttgtgta tagtcgctgt tttgggctac cattgcgccc ggtgcggcag ctcgcccata
    10501 cattacatta tcataatgat aagttaacat agtctgaaca tacggcgcct tatgattgtt
    10561 ttttcctcgt tacaaattcg tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc
    10621 atcaaccctg ggcagaaagt cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg
    10681 ctggcattgc tgaaaaatga aatcagcgcc gacggcggca gctacacctt tccgggaagc
    10741 tggcaactgg cgtgggtgaa tcaggaaaca ccggcgttac cgcaagcggc gctggaatat
    10801 gtcattgacg gcgaccgtga atatcgtcaa ctggaagcgc agctacacga cgccaacgaa
    10861 cgtaacgacg ggcacgccat tgcgaccatt catggcaagc tggatgctat tgacgcatgg
    10921 agtattcgct cccgtgccgc cagcctgctg cacggcctcg gtttcagcaa tgaacaactg
    10981 gagcgcccgg taagtgattt ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg
    11041 ctgatttgcc gttcagactt gctgctgctc gacgaaccga ctaaccacct cgatctcgat
    11101 gccgttatct ggctggaaaa atggctgaag agctatcagg gcacgctgat cctgatctct
    11161 cacgatcgcg atttcctcga tccgatcgtt gataaaatta ttcatatcga acaacaaagc
    11221 atgttcgagt acaccggcaa ctacagttcg tttgaagtac agcgcgccac ccgtctggcg
    11281 cagcaacaag cgatgtatga aagccagcag gaacgcgtag cgcatctgca aagttatatc
    11341 gaccgtttcc gtgccaaagc caccaaagcg aagcaggccc agagccgcat taagatgctt
    11401 gagcgtatgg agctgattgc ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc
    11461 gcgccggaaa gcctgccaaa tccgttactg aagatggaaa aagtcagcgc aggctatggc
    11521 gatcgcatta ttctcgactc gattaaactg aatctggtcc ccggctcgcg cattggtctg
    11581 ctaggccgca acggcgcggg taaatcgaca ttaatcaaac tgttagccgg ggaacttgcg
    11641 ccagtcagcg gtgaaattgg tctggcgaaa gggatcaagc tcggctactt cgcccagcat
    11701 caactggaat acctgcgcgc cgacgaatcg cctattcaac atctggcacg tttagcgccg
    11761 caggagctgg agcaaaaact gcgtgactac ctcggcggct ttggtttcca gggcgataaa
    11821 gtaaccgaag aaacgcgccg cttctccggt ggggaaaaag cccgcctggt gctggcatta
    11881 attgtctggc agcgtccgaa tctgctgctg ctcgacgaac cgaccaacca ccttgacctc
    11941 gacatgcgtc aggcactcac cgaagcatta atcgagttcg aaggcgcgct ggttgtcgtc
    12001 tcgcacgacc gtcatttgct gcgttccacc actgacgatc tctacctggt tcacgatcgt
    12061 aaagtcgaac cgttcgacgg cgatctggaa gattatcaac agtggttgag cgacgtacaa
    12121 aagcaggaaa accagaccga cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt
    12181 aaagatcaga agcgtcggga agcggagctg cgtgcgcaaa cccagccact gcgtaaagag
    12241 attgcccgtc tggaaaaaga gatggagaag ctgaacgcgc aactggcgca ggcggaagag
    12301 aaactcggcg acagcgaact gtacgatcaa agccgtaaag cggagttgac cgcctgcctg
    12361 caacagcaag ccagcgccaa atccggcctg gaagagtgcg aaatggcatg gctggaagcc
    12421 caggagcagc ttgagcagat gctgctggaa ggccaaagca actgatggcg cagataacaa
    12481 cgaccgatgc caatgaattt agcagcagtg ctgaattcac ccccatgcgc ggctttagca
    12541 attgtcatct acaaaccatg ctgccgcgtc tgttccgtcg caaggtgaaa ttcaccccgt
    12601 actggcagcg gctggagttg cccgacggcg attttgtcga tctcgcgtgg agtgaagatc
    12661 ctgcacaggc gcggcataaa ccgcgtttag tggtatttca cgggctggaa ggcagtctca
    12721 atagccctta cgctcacggt ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg
    12781 tgatgcattt tcgcggatgc agcggtgaac caaaccgtat gcaccgcatt taccattcgg
    12841 gcgaaaccga agacgccagt tggtttttac gctggctgca acgcgagttt ggtcatgcgc
    12901 caacggctgc cgtcggctat tcgctcggcg gtaatatgct ggcctgtttg ctggcaaaag
    12961 aaggcaatga tctcccgatt gatgcggcag tgattgtctc tgcgccgttt atgctggagg
    13021 cctgtagtta ccatatggaa aagggctttt cccgcgttta tcagcgttac ttgctgaacc
    13081 tgttaaaagc caatgccgcg cgcaagctgg cagcctaccc cggcacactg ccgattaatc
    13141 tcgcgcagtt aaaatcggta cgtcgcatcc gtgaatttga cgatctcatc accgccagaa
    13201 ttcacggcta cgctgacgct atcgactatt atcgtcagtg tagcgccatg ccgatgctca
    13261 atcggatagc caaaccgaca ctgattattc acgccaaaga cgatccgttt atggatcatc
    13321 aggtgatccc gaaaccggaa agtctccccc cgcaggtgga gtatcagctg actgaacatg
    13381 gcggtcatgt tggctttatt ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac
    13441 gcattcctga ctggttaaca acgtatctgg aggcgaaatc atgttgattc cgtggcaaga
    13501 cctctccccc gaaacgctgg aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga
    13561 ttatggtgaa catgagcgta cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca
    13621 gtgcggagaa gcggtgctgg tatggtcgga gctgcacgaa acggtcaata tcatgccgcg
    13681 cagtcagttt cgcgaataac ctgcaaccgt cggcggacga gataataatg atagctaagt
    13741 catggagtta ctatgtctgc caaacatccg gtcattgcgg taacaggatc cagcggcgcg
    13801 gggaccacca ccaccagcct cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca
    13861 gccgaggtgg aaggcgacag ttttcaccgt tatacccgcc cggaaatgga catggcgatc
    13921 cgcaaagcgc gcgacgccgg gcggcatatc agctacttcg gccccgaagc gaacgacttc
    13981 ggccttctgg aacaaacctt cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa
    14041 tatctgcata cctacgatga agccgtaccg tggaatcagg taccggggac attcaccccc
    14101 tggcaatctt taccggaacc cactgatgta ctgttttatg aaggtttaca cggcggcgta
    14161 gtcacgccac agcataacgt tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc
    14221 gttaaccttg agtggattca aaaactgatc cgtgacacca gcgagcgcgg gcactcacga
    14281 gaagcagtga tggactcggt agtgcgttca atggaagact atatcaacta catcacaccg
    14341 cagttttccc gcacccatct taacttccag cgcgttccca ccgtcgacac ctcaaacccg
    14401 ttcgcggcaa aaggtatccc gtcgctggat gaaagctttg tggtgatcca ttttcgtaat
    14461 ctggaaggga tcgatttccc ctggctgctg gcgatgttgc aaggctcatt catttcccac
    14521 atcaatacgt tagtggtgcc tggcggcaaa atgggtctgg caatggaatt aattatgctg
    14581 ccgctggtgc aacgattgat ggaaggaaag aaaatcgagt aacgctgcta ttacgccgga
    14641 taaaatacta tccggcttca caacgggata gttaaatcac gcggcaacca cttcatacga
    14701 gtgagtaata ttcaccgctt tttccagcat cagcgccact gaacaatatt tctcggcaga
    14761 gagatcaacc gcacgcgcaa ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg
    14821 cagattaatg tgcgtaaaca ggcgtggtgc ctcttcgcgg cgctcagagg tcaattttac
    14881 ttcacaatcg accacatcct gacgcccttt ttgcaggata gaaaccacat cgatggcact
    14941 gcaaccaccc gccgccatca acaccatttc catcgggctt ggtgctttat cgcctgagtt
    15001 gccgtccatt aaaatctggt gaccagaggc ggattcgccc agaaaagtta acccttcgac
    15061 ccacttcact cgcgcttgca tttttgctac tccactgcgt caattttcct gacagagtac
    15121 gcgtactaac caaatcgcgc aacggaaggc gacctgggtc atgctgaagc gagacaccag
    15181 gagacacaaa gcgaaagcta tgctaaaaca gtcaggatgc tacagtaata tattgatgta
    15241 ctgcatgtat gcaaaggacg ccacattacc gtgcagtaca gttgatagcc ccttcccagg
    15301 tagcgggaag catatttcgg caatccagag acagcggcgt tatctggctc tggagaaagc
    15361 ttataacaga ggataaccgc gcatggtgct tggcaaaccg caaacagacc cgactctcga
    15421 atggttcttg tctcattgcc acattcataa gtacccatcc aagagcacgc ttattcacca
    15481 gggtgaaaaa gcggaaacgc tgtactacat cgttaaaggc tctgtggcag tgctgatcaa
    15541 agacgaagag ggtaaagaaa tgatcctctc ctatctgaat cagggtgatt ttattggcga
    15601 actgggcctg tttgaagagg gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg
    15661 tgaagtggct gaaatttcgt acaaaaaatt tcgccaattg attcaggtaa acccggacat
    15721 tctgatgcgt ctgtctgcac agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg
    15781 caacctggcg ttcctcgacg tgacgggccg cattgcacag actctgctga atctggcaaa
    15841 acaaccggat gctatgactc acccggacgg tatgcaaatc aaaattaccc gtcaggaaat
    15901 cggccagatt gtcggctgtt ctcgtgaaac cgtgggacgc attctgaaga tgctggaaga
    15961 tcagaacctg atctccgcac acggtaaaac catcgtcgtt tacggcactc gttaatcccg
    16021 tcagagtggc gcgttacctg gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg
    16081 atttatcacc ccgatatcaa ctatgcactt cgacaaacgc tggtgctatg tttgcccgtg
    16141 gccgttgggt taatgcttgg cgaattacga ttcggcctgc tcttctccct cgttcctgcc
    16201 tgttgcaata ttgcgggcct tgatacgcct cataaacgtt ttttcaaacg cttaatcatt
    16261 ggtgcgtcgc tgtttgccac ctgtagcttg ctgacacagg tgctactggc aaaagatgtt
    16321 cccctgccct ttttgctgac cggattaacg ctggtacttg gcgtcactgc tgagctgggg
    16381 ccattgcacg caaaattgct tcccgcatcg ctgctcgccg ccatttttac cctcagtctg
    16441 gcgggataca tgccggtctg ggaaccgttg ctcatctatg cgctgggcac tttgtggtac
    16501 ggattgttta actggttttg gttctggatc tggcgcgaac aaccgctacg cgagtcacta
    16561 agtctgctgt accgtgaact ggcagattac tgtgaagcca aatacagtct gcttacccag
    16621 cacaccgacc ctgaaaaagc gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat
    16681 ttaattaccc agtgctatca gcaaatgcat atgctttccg cgcaaaataa taccgattac
    16741 aagcggatgc tgcgtatttt ccaggaggcg ctggatttgc aggaacatat ttcggtcagt
    16801 ttgcatcagc cggaagaggt gcaaaagctg gtcgagcgta gccatgcgga agaagttatc
    16861 cgctggaatg cgcaaaccgt cgccgctcgc ctgcgcgtgc tggctgatga cattctttac
    16921 catcgcctgc caacgcgttt tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc
    16981 cgccaacacc cggataatcc ggttgggcaa ttctgctact ggcatttcag ccgcatcgct
    17041 cgtgtgctgc gcacccaaaa accgctctat gcccgtgact tactggccga taaacagcgg
    17101 cgaatgccat tacttccggc gctgaaaagt tatctgtcac taaagtctcc agcgctacgc
    17161 aatgccggac gactcagtgt gatgttaagc gttgccagcc tgatgggcac cgcgctgcat
    17221 ctgccgaagt cgtactggat cctgatgaca gtattgctgg tgacgcaaaa tggctatggc
    17281 gcaacccgtc tgaggattgt aaatcgctcc gtgggaaccg tggtcgggtt aatcattgca
    17341 ggcgtgacgc tacactttaa aattcccgaa ggttacaccc tgaccttgat gctgattacc
    17401 accctcgcca gctacctgat attgcgcaaa aactacggct gggcgacggt cggttttact
    17461 attaccgcag tgtataccct gcaactgttg tggctgaacg gtgagcagta catccttccg
    17521 cgtcttatcg ataccattat tggttgttta attgctttcg gcggtactgt ctggctgtgg
    17581 ccgcagtggc agagcggatt attgcgtaag aacgcccatg acgctttaga agcctatcag
    17641 gacgcgattc gcttgattct gagcgaggac ccgcaaccta cgccactggc ctggcagcga
    17701 atgcgggtga atcaggcgca taacactctg tataactcat tgaatcaggc gatgcaggaa
    17761 ccggcgttta acagccatta tctggcagat atgaaactgt gggtaacgca cagccagttt
    17821 attgttgagc atattaatgc catgaccacg ctggcgcggg aacaccgggc attgccaccg
    17881 gaactggcac aagagtattt acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga
    17941 ctggagtatg acgaaccggg tagttctggc gatgccaata tcatggatgc gccggagatg
    18001 cagccgcacg aaggcgcggc aggtacgctg gagcagcatt tacaacgggt tattggtcat
    18061 ctgaacacca tgcacaccat ttcgtcgatg gcatggcggc agcgaccgca tcacgggatt
    18121 tggctgagtc gcaagttgcg ggattcgaag gcgtaataca ggctaaacca tcggatgagg
    18181 tgttcacgcc gcatccgaca tcttttgcct gatgcgacgc tgacgcgtct tatttacgcc
    18241 ccaatcacct tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcaat atccgcatct
    18301 tccaccacca gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg
    18361 ccttcctctg cacccgcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc
    18421 tctgcgccaa tcaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg
    18481 atcttctgca aatggtcaac aaaacgctgg cgtttcgcct gaataccttc cagcacttca
    18541 ggggtattga tgatatcaaa cgctgcgccc gctactgcac aggccagagg attaccgccg
    18601 taggtggaac cgtgagaacc aggatgaaac gcggaagcaa tctccgccgt ggtcagcatg
    18661 gcgctaaccg ggaaaccgcc acctaacgct ttcgcagagg tcagaatatc cggcgtcacg
    18721 ccgtagtgca tataggcaaa cagatcgccg gtccgcccca tcccgcactg cacttcatca
    18781 aacaccaata atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc
    18841 gtcgctgccg tcacgccgcc ctcgccctga atcggctcaa ccaccaccgc acaggtgtga
    18901 tcatccatca ccgctttcac tgcgtggaga tcgttaaagg gaacgtggat gatgtctgcc
    18961 ggttttggac caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc
    19021 gagcgaccat gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc
    19081 acacaggcgt aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc
    19141 ataaacacca cgcgttcggc aaacgttgcc tcaatcagtt tacggccaag acgcagcgcc
    19201 ggctcattgg tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg
    19261 ttcaccagcg caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg
    19321 acatactcct tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac
    19381 tctgccggag cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt
    19441 tgttcaattg ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca
    19501 caaaatatgc ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc
    19561 cagcagttga tgtccttgtt cgctaagaat actttctgga tggaattgca caccttccag
    19621 atcccactgg cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc
    19681 aaagcactcc ggtaatgagt caggttccac taccagcgaa tggtagcgtg tcacggtgag
    19741 tggatttgcc agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc
    19801 gtgcatgacc tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg
    19861 accgaggcag acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga
    19921 gatcccggct tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag
    19981 ggcgtcgata tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag
    20041 ttcacaaaag tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat
    20101 catggcggct ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta
    20161 ccactttgct aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg
    20221 cctgattcca ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg
    20281 ccagttgctc gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca
    20341 cgttgatttc acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa
    20401 ccagataacc ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag
    20461 caaagtggca gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc
    20521 agtcgaaaat atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa
    20581 ccaacgggcc aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt
    20641 ccagccgctg ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa
    20701 gatacggatc gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaagg
    20761 cgcgctaaag cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg
    20821 cgactggcct ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg
    20881 gtgagtttct tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca
    20941 aatccgggtt acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt
    21001 acggcaggac tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac
    21061 caacgtcatg agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt
    21121 taccaaatac cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa
    21181 cgttgataaa gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg
    21241 tgccacgcgt gttgcgcagg ccgttatctg cttcattttt gattggcggg tttggttttt
    21301 tctgctgcat ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt
    21361 gaaaggtcgt gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca
    21421 ctggcgcttt ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata
    21481 cgtgcgggtc ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag
    21541 cagccatcgc cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa
    21601 aaagcaagtg aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt
    21661 tacgtatctg aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct
    21721 cttaaaatgc aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc
    21781 taaaatcgcc cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat
    21841 catgactaac agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac
    21901 cggtgcgttg gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct
    21961 gcctgtttcc agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat
    22021 cttcctcaac gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt
    22081 tctcctgatg gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc
    22141 ggcggcgatg ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct
    22201 ggtaacacaa atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt
    22261 cttcagtatg gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat
    22321 tgagtggtac tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac
    22381 cttcggctgt gagttcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga
    22441 gaaagaaaag tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct
    22501 cggtcagtta ggttttatct cctgggtgcc tgagtatgcc aaaggcctgg gcatgagcct
    22561 gaacgacgcg ggcacgctgg tgagtaactt ctggatgtca tacatggtcg gcatgtgggc
    22621 gttcagcttt attcttcgct tctttgattt gcaacgcatt ctgaccgtac tggctggtct
    22681 ggctgcgatt ctgatgtacg tctttaacac cggaacaccg gcacatatgg cgtggtcaat
    22741 tctcgccctg ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca
    22801 gcagaccaaa gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg
    22861 tactatgttg acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc
    22921 gctgctgacg gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt
    22981 cgtcagccgt caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg
    23041 cactatcgtc gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat
    23101 ccgttcaatt tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag
    23161 aaaatttata caaatcagca atatacccct taaggagtat ataaaggtga atttgattta
    23221 catcaataag cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc
    23281 aaaaatgagc aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat
    23341 cgaagatctt cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga
    23401 accgcgcatc gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga
    23461 agagctgtcg ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg
    23521 cgaacgcgct atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac
    23581 cgttttttat gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaattaa
    23641 aggttctgat actcaggact gctttgtcta tcgcaccatt gaagacctca acgccattga
    23701 atcctgcgct cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt taggtctgga
    23761 agctgctggc gcgctgaaaa acttaggcat tgaaacccac gttatcgaat ttgcccctat
    23821 gctgatggcg gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag
    23881 tatgggcgtg cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg aaggtgttga
    23941 agcgcgtaaa accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt
    24001 ctctaccggt atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc
    24061 gcgtgggggt atcgtcatca atgattcctg ccagacttcc gatccggata tctacgccat
    24121 cggtgaatgc gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat
    24181 ggcgcaggtc gccgttgacc atattcttgg tagcgaaaac gcctttgaag gtgcagacct
    24241 tagcgccaag ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg
    24301 cacgcctggc gcacgtagct acgtttacct cgacgaaagc aaagagatct acaaacgcct
    24361 gattgtcagc gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga
    24421 ctacggcaac ctgctgcaac tggtgctgaa cgctatcgag ctgccggaaa acccggattc
    24481 cctgatcctg ccggcgcact cgggtagcgg caagccgtct atcggtgttg ataaactgcc
    24541 ggacagcgcg caaatctgct cctgcttcga cgtcaccaaa ggcgacctga ttgctgctat
    24601 caacaaaggc tgccacaccg ttgcggcgct gaaagccgaa accaaagcgg gtactggctg
    24661 cggtggctgt atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat
    24721 cgaagttaac aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt
    24781 gatccgcgtt gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggtta
    24841 cggttgtgaa gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata
    24901 cattctgaag ccggaacata ctccgctgca ggattctaac gacaacttcc tcgcgaacat
    24961 ccagaaagac ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga
    25021 agggctgatg gcagtgggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg
    25081 ctctcagcgc ctggcgatgt ttggcgcaca gaaagacgat ctgccagaga tctggcgtca
    25141 actgattgaa gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa
    25201 aacctgcgtg ggaagcacct ggtgccgcta cggtgttggt gacagcgtcg gcctcggcgt
    25261 ggaactggaa aaccgttaca aaggcatccg tacgccgcac aaaatgaagt tcggtgtctc
    25321 cggctgtacc cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccaccga
    25381 aaaaggctgg aacctgtacg tttgcggtaa cggcggcatg aaaccgcgtc atgcggatct
    25441 gctggcagcg gatatcgatc gcgaaacgct gatcaaatat cttgaccgct tcatgatgtt
    25501 ctacatccgt actgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg
    25561 tggcatcgat tacctgaaag cggtgatcat tgacgacaaa ctggggctga acgcacatct
    25621 ggaagaagag atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa
    25681 taccccgtct gcgcagactc gcttcaaaca cttcatcaac agcgataagc gtgacccgaa
    25741 cgtgcagatg gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc
    25801 ggtaactctg gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac
    25861 atcctgcctg aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc aattttccgc
    25921 ccgtatcaca gcgatcaggt gtttgcgatc agcaacatcg acccgttctt cgagtccagc
    25981 gtgctgtcac gcgggttgat tgcggaacac cagggcgagc tgtgggtcgc cagtccgctg
    26041 aaaaagcagc gttttcgctt aagcgacggc ttgtgcatgg aagacgaaca gttttccgtc
    26101 aagcattacg acgcgcgagt gaaagacggc gtggtacagc tgcgcggtta atattttaac
    26161 gggaggcgca atgccttccc tttttgcatg gtcctgtaat aaccttcggt atattgcagg
    26221 acatttttta aactttttgt tttatttttt gtttttattt tttaaaggat aatcaaatgt
    26281 ttacagacac tattactaag tgtgcggcta acgctgcgcg cattgcacgc ctgtcggcaa
    26341 ataacccgct cggcttttgg gtcagctccg cgatggcggg cgcgtatgtg ggtcttggga
    26401 tcatcctgat tttcacgctc ggtaatttgc tcgacccgtc cgtacgtcct ctggtgatgg
    26461 gcgcgacctt tggtatcgcc ttaacgctgg tgattatcgc cggttctgaa ttgttcaccg
    26521 gacacaccat gttcctcacc tttggggtga aagcgggcac catcagccac gggcaaatgt
    26581 gggcaatcct gccgcaaacc tggctgggta acctggtcgg ttccgtcttc gttgccatgc
    26641 tttatagctg gggcggcggt agcctgctgc cggtagatac cagcatcgtt cactccgttg
    26701 cgctggctaa aaccactgca ccggcaatgg tactgttctt caaaggtgca ttgtgtaact
    26761 ggctggtttg cctggcaatc tggatggcgc tgcgcactga aggggcggcg aaatttatcg
    26821 ctatctggtg gtgtttgctg gcatttatcg cgtccggcta cgagcactcc atcgctaaca
    26881 tgacgctgtt cgcactctcc tggttcggca accacagcga agcctacacg ttggcgggca
    26941 ttggtcataa cctgctgtgg gtgacgctgg gtaatacttt atcgggtgcc gtattcatgg
    27001 gattgggtta ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac aaatttaatc
    27061 aaactgaaac ggctgccggt taattactaa ggggttttta cgtggatcat ttgcctatat
    27121 tttgccaatt acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc gcggaacgca
    27181 aagcaaggtt gctgttagac gcaggcgctc gcttaacggt gaatgcatta gcgtttattc
    27241 cacagttcac cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg ccatttgatg
    27301 aaagccttct cgacacctgc tggctggcga ttgcagcgac agatgatgac gcgcttaacc
    27361 agcgcgtcag cgaagccgct gaagctcgtc gcatcttctg taacgtggtc gatgcgccga
    27421 aagccgccag ctttattatg ccgtcgatta ttgaccgctc accgctcatg gtagcggtat
    27481 cctctggcgg cacctctccg gttctggcgc gcctgttgcg cgaaaaactc gaatcactgc
    27541 tgccgttaca tctgggccag gtagcgaaat acgccgggca attacgcggg cgagtaaaac
    27601 agcagttcgc cacgatgggt gagcgtcgcc gtttctggga gaaattgttc gttaacgacc
    27661 gcctggcgca gtcgctggcc aacaacgatc agaaagccat tactgaaacc accgaacagt
    27721 taatcaatga accgctcgac catcgcggtg aagtggtgct ggttggtgca ggtccgggcg
    27781 atgccgggct gctgacactg aaaggtctgc aacaaattca gcaggcagat gtggtggtct
    27841 acgaccgtct ggtttctgac gatattatga atctggtacg ccgcgatgct gatcgcgttt
    27901 tcgtcggcaa acgcgcggga taccactgcg taccgcagga agagattaac cagatcctgc
    27961 tgcgggaagc gcaaaaaggt aaacgcgtgg tgcgtctgaa aggcggcgat ccgtttattt
    28021 tcgggcgtgg cggagaagag ctggaaacac tgtgcaacgc gggtattcca ttctcggtgg
    28081 ttccgggtat taccgcagct tctggttgct ctgcctattc gggtattccg ctcacgcatc
    28141 gcgattatgc ccagagcgta cgcttaatta ctggacactt aaaaaccggg ggcgagctgg
    28201 actgggaaaa cctggcggca gaaaaacaga cgctggtgtt ttatatgggg ctgaatcagg
    28261 ctgcaactat tcagcaaaag ctgattgaat acggtatgcc tggtgaaatg ccggtggcaa
    28321 ttgtcgaaaa cggaacggca gtcacgcagc gcgtgattga cggtacgctc acgcagctgg
    28381 gcgaactggc gcagcaaatg aacagtccat cgctaattat tattggtcgg gttgttggcc
    28441 tgcgcgataa attgaactgg ttctctaacc attaatttaa ccagggccag agaagtctgg
    28501 cctttttcgt ttacaactct ctttatatat tcagcagata aaaacatacc cccttaataa
    28561 atattgtctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aaatcaatcg
    28621 ctcactgacg ctaattgaat attcctgtat cgttcttaat gagcagtctg cttatattca
    28681 tataatcaat gaatattaat taatatataa tacataggga atgtaaatga acaaatttat
    28741 taaagttgca ctggtaggcg cagtactggc tacgttaact gcatgtactg gtcatattga
    28801 aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa
    28861 aatcattggc ggttgcggtc cgactgcaca gtaagctatt gacagaaatc ggctaacgcc
    28921 ggtttaattg cctgacgccc tcggagttat ccgagggcgt atttgtttct gcattctttt
    28981 caaaaatatc gtttacaacg attaaatgct ggcaatactt tcacgcaaga tccgcaatat
    29041 cgtctcttca ccgccgcgat ttgggttaat gcgaatcgcg caatgttcta actgcggatt
    29101 cgcctggcga aacgttccgg aaagtcgata aaagagcggc gggatttcat atttcgactc
    29161 tgcaccaacc gggtaaggca aggcaccgag tttctgcgcc tcttccagca ctctgacggc
    29221 aatcggctga tgaaactcca caatcaatac cttcgactgt gcattggcaa tgacggcgct
    29281 tttcacttcc gccaccgcgc cgccgttaag caaagccagc aaccgttcag atacccccgc
    29341 ctgcaccgcg tgcatcactg gcgcgagcac cagaccacgc aacacctcta atgcctgcgc
    29401 cccctgaatc tgactgccgc cggaatagag cgtggcgcga atccggctaa taacatcggc
    29461 atcgcctacc accgcaccaa caccctctgg cccaaatagc ttgaagcagg aaaatgtcga
    29521 cacattcgcc ccgcattcgc agccgattcg cgctaccttc atcaccgcat agttgtcatc
    29581 ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc acgtctgcca gcacatagcg
    29641 gtcctgcggc tgctggcgcg tatgctgcac cagcgctgca tcaggttgtt gctcgtcaac
    29701 aacctgcttc agtgccgaca gatcattgaa atcagccgta atgagcgtta gtcccatctg
    29761 ctcaataata acccgtgtcg ttgggtaaac cggcgcgtca tgcaccagaa gacgttgccc
    29821 cggtttgagc aaagccgcca gtgccgcacg gatcgcgcca gtacccgcgc cctgtaccag
    29881 cgccgcggcc tgtgcgtgaa acgcgtcagc cagcacctgt tccacacgct gggtaatgcg
    29941 gggctgattt agccccggcg ttaagcccaa atcaccgcag gtaagaaact cgctgccagg
    30001 aaaatgacgg caaatcgtgt ccaccagcgc aaactgcttt tgctgcgcct caataagcgt
    30061 caggctttgc agaggaaacg tcttcatagg tgccttcctt aagccggaac gaacaggcca
    30121 agccagtaaa gaacattcag caggatgccg gtgatcatta ctgctacgac cggtgccgcc
    30181 attttctgta ccggacggcc cagggattcg ttgaggaagt aaatggcaac cgcgatagag
    30241 aatccggtat agcccgccat tttgattgct gcaaagatcg aaccgaccag caacgccact
    30301 tccatcagca tattcatggc gttacggatg ttatccgacg cattacgtac cgacgggtag
    30361 cgccccaacc atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg
    30421 cctaataccg ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta
    30481 aagcccgcaa ctgcatacac accggtggct aacgcggtgg tggcaatcaa cggcacaaaa
    30541 cccagcccgc gcataaattc tgccagtgcc gcctggttga ttagtgtttg cgattgttcc
    30601 ggcgttacgc ctgcggagta cgctttctcc agtgtgaaga tcgacacttc actaccggcg
    30661 aaaatcttca tgctggcaac ggcagcaatc aatgcaccca cgatggcgat atacggtaag
    30721 tttttgataa tccgtgatgt acgctcttca aacaccgaca taccgctggc atcgatatca
    30781 ttttcatcac gatggcgcag gtcatgagtt attgcgatcc ccagcagcat caccatgcca
    30841 ataaagattt cgatggattc agggttaaga tgtgggaaat agcgcactac caccacacga
    30901 gtcatcagta ccaccacggc ggcgatcaga ctttgcttcc agccaaactg gtagaaaatc
    30961 gccaccaacg ggaacagtgc aaaagctgaa accaccggcg agcttaattc ccccaggcta
    31021 cctaatacat ccaccggtag cgcggtcagc agctggttta ctggcaacag acaggtgagg
    31081 atcaatattc cccagatagc accaagacca aacgccatca ggctgtttat cgccagtacg
    31141 ccgaggatat cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta
    31201 aaagagatcc ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc
    31261 tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga tcgggcggat accgtcatga
    31321 aaaacggctg ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg
    31381 ataatctgaa tgtacagatc catagtcgtg ccttttattt cagatggtta atcagcatcg
    31441 gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc cacttttcct tcagcgatca
    31501 ttttggcgat atgttcgtct ttcgctttaa tgcccggctt ggcaatggtg caacttttgt
    31561 tatagccaat caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc
    31621 cgatgtagta atccagttgt ccggatttca ctttcattgc cgcttccatg tcgttgtgaa
    31681 tgaaaacttc aaaacagcca ggagccgtcg cttcaatggt ttttttaatc tgctcacgct
    31741 gtaagcctgc aacgccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga
    31801 ttttcacgta acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga
    31861 ataaaggtgg tcaataaaaa gtcatagcca taaccaccgt tggcttttaa atgagagcgc
    31921 cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc cacggtcacg tagcgcatgg
    31981 agcatcgcaa tgcgcttttc gtccgggtag taactgttct tgccgatggt gtcgaactgc
    32041 acatacgcgc cgagatcgat catcttcaaa atgttgtcga gattgtcttt cagatcgcag
    32101 tggccgacgg tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgctcc
    32161 agccccatcg tgctgaacga cgtatgcgtg gagatcgggc gtccggtctg gttatgtgcc
    32221 agcgcggcgg caataaacac cttctcttcc agcggcgtaa tcttcccttc gctggagcca
    32281 atctccgcga tgatcccggc tttcaggtca gtgccatcga taccctgctc aatttcatcg
    32341 accatctctt gcgccagttc ctgcgcgctg cgggtcgcca catgttccgg gaaaaacgcg
    32401 tcctggtaat aaccggtaca ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc
    32461 ataaattgcg cattgcgccc catgtaacgg ttggtcatct caatcacatt acgcacgccc
    32521 tgggccatca ggtcgttcat ctcctggcaa atgaacgcat actgatcaag gcggcagtcc
    32581 acgttgtttt taaagccgga gagatcaata tgcagatgct catgggcgag ggtgtagccc
    32641 gtcggatcaa aactcatagg gtgtctcctg caaaccggag ggaggaaaga aaagagcgac
    32701 cattttgtgg cggtggcgca cggaaaaatt cacacacggt agcccccaca tcagaaagcg
    32761 tggtgcgcac accgagttgc gtgtggacca gcccttgctg ataaaccagc actggcacca
    32821 cttcacgggt atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga
    32881 ccaggcaatc atctggctgc atcgcctcaa caagccgggc aaggttacgg tcaacgacct
    32941 gcaaacgttc ggcataacgt gcgacgtctt ccgcgtgacc agcgaggtcg gtttcctgga
    33001 tattggtgca aataaacgcc gtcggatgcg tgttaaattc gtcgagggtg atatccataa
    33061 tccgctggct atccaccagg ttttgccagc tcacgccata aggattactg gcaatatccg
    33121 ccactttccc caccagcacg gttggtacgc ccacttcata cagtttttgt ggcacctgca
    33181 ctttatcatc gacaccgtag cccatatgca cgacctggaa gccgttgtca taagcgcctg
    33241 aacgcggcgc attgatacca ataaagcgcc cttctttgct ttctgcggca tcgagaatgc
    33301 gttgactgtc gggtaacagg ccgccaaatg taatgacccg accgacctgt acctgctcac
    33361 gcacaatacg accaattttg attgcgtcgt caaaagagat cacagagaga ttggcggtga
    33421 tgttatagac ctggcctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga
    33481 cccacagaaa ttgcagatca tcgccacggc gctccacctg ccagccagcg gaaactaatg
    33541 cctgctcaac acggtcaatc acatcgcgaa agggcatccg cagcggcggt aacgggcgcg
    33601 tgcctaaaat ttcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca
    33661 ccccccaggt tgcggaatct gacggttgca tatcacctgg tgcataaccc aatgcgttga
    33721 ttagccccag cttctccagc gctggtagct gcaaatgcgg caactggctc aggatgtgac
    33781 cacaggtatt cgccccggca tcctgcggac gcaccagcgt gacatctttc attgccccta
    33841 cgccaaagct atcaatcact aacaccacaa atcgcgccat ttacgcctcc agagggttgc
    33901 cgaggctgtc ataacgacca actatttctg gttcgccatg gtgaataccg gacaccagca
    33961 ccacatcgct acgggtgata aaaatctgcg tgcgaaaaca gagcaccact gcactactta
    34021 ccggatgctc gcctgccagc ggcagggtgt agtcgatact gctgtcatcc acggcgttga
    34081 gatatgtttc agtaattctt tgattttctg gcgtaaacac cagcgcatgt tgcgcgtgac
    34141 cacgacggta gtaaccgccg ccgtagcagt agctgtcgcc acggaaatga tgggagattt
    34201 cacttaacca gagcatcgcg atacgttcag gctgatcgcc ctgttgattt gccggaatag
    34261 tgcccgtcag cgcatgaccg ggttcggtat gagtcacacc gtattccgcc agcaatggca
    34321 gcgacgtgca gctggtcgct gaaggtgcgt tcagttgctc aatcgcaata ccagatttcg
    34381 ccagttgatc ccgtgcttgt accagcgtgt gaagattcgg tgtcggcaaa actttcccgg
    34441 cagcctcatc ccaaagcagg caagggaaat gggtaagtcc cgccaaatgt agccccggca
    34501 gattctggat ttcagcaacc acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc
    34561 ccggataaag aaaatcatcg tcgctataaa ctttgagcag cacagactgc acacgtccgg
    34621 cctccaccgc tgccgcagaa acttcccgcg ctttgtcgag agtaaacacg gtgatgacgt
    34681 cggttccctg tgcaacggcg tcaaccacct gatgacaagg gatttgtacc aaatgcccct
    34741 gatgcgctac cggcaaaccg gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca
    34801 caatgccgct gtagcccaga gccagcagtt tttctgccag ccacggatta cgaccgaatt
    34861 gtttggtcat cagatacagt tcaatgccgt aaagccgcgc cgtctcaatc agccgcttgc
    34921 cgttttccag aacttgatcc acgtcgatca cccaactgtc cggggcgatc ttgccctgct
    34981 gccacaaact tagtgcggcg gaaatcagcg ccggattctg gcgtttcaat gcttctacaa
    35041 acatcttgtt gcctcgtttc tgttccttga attaaatatt catttttttg aatatttagg
    35101 ttgtacgacg tggatttatc gggagcttca aacctcttcg ttagcagcca tccataatcc
    35161 ataaaggttg gccagcaaat agccttcttc acacgggtta acttccagtg caaattcctt
    35221 caacaatacc tgatgcaatt gcacaacggc ttgccagtgg ctggattgcg ccagttccgc
    35281 cagcaattcg tcatccagcg gctctatttc ttcaccacgg cgactgcgca tcagtgcact
    35341 cgccatatgt gtcatcgcca tcgttccttg ctcactgcgc accggtagat ggcactctgt
    35401 ttccagtaca ttgacgacct gcatcatgcc tttgcagatg tccttatcaa taacgcctgc
    35461 atcgcaaagc aggttgagtc tggtttccat atcagatgcc tttcgtttaa taactgggtt
    35521 cctgttcccc actgactact cgctgttgat gagcaagcag ggcatgttta tcaacaactg
    35581 cacgcagcag ttgttgcatt gggtaatcat cggcacgcgt caggatcacg gcttcggtgg
    35641 cctgtaaaaa tcgcggatcg tctgtgagcg gcgtcgcttc taatcccagc atagtcagtt
    35701 cgttttccgc cactacgttc cagataaccg cgtcaacatc gcctttaaca atgcgttgta
    35761 aactctcgtg ataagagaga tcgactcgtt ccacatcact atcgccaaaa aagacatcgg
    35821 tcatgatttt ctgatctgcc gaacggttat ccagccccac gcgcttcacg tttgcggact
    35881 cgcctttacg gcaaatcaac tggtgctcgc caacgtaggt atgcggtccc aactccagcg
    35941 cgaggcataa tcctttttgc gtgagataac tttccgccgc cagtcgcgaa accaccgcca
    36001 tgtcatacac gccgttaagt aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat
    36061 agtaaaaagg aatgccatca aactgggctt tcaatccgct cgccaggcct tcgtacaaac
    36121 gggtataggg caagggcatc gcacatacca cgttgttgat atccacatga gtcagcaatg
    36181 ctttgttatc catctcgacc agataactgc cattgcgccc acggcgttca atccgtatcg
    36241 ctccgcttga ttccagcgtt ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt
    36301 cagttgccag ctcatctatg gttttcagtc gattaccgca cttttcaccc aacaaataac
    36361 gggccagtgt cgtgataacg acgccttctt ttttgataaa cgttcgacgc ataatatatt
    36421 ttcaataaaa tgaatgttta tatcttcagg aatttgaaga taaggcgcaa cagcgagatg
    36481 tggaaacggc gaggcgcttc acattttttc ggattattgc aaatgaggat aaaaaaaccg
    36541 ggtttccccg gtttttggca ttaatgttaa attacggctt cgccacaaaa ccaatcgctt
    36601 cgtacaccgc ttttagcgta cgggaagcgt gtgcgctggc tttttctgcg ccgtctttca
    36661 tcacctgttg caggaaggct tcatcgttgc ggaaacggtg gtaacgttcc tgcaattcag
    36721 tcagcatacc ggaaacggca tcagccactt cacctttcag atgaccatac atcttgcctt
    36781 cgaactgttt ttccagttcc gggatgctct ggcccgttac cgcagaaagg atatccagca
    36841 ggttggaaac gcccgctttg ttctgcacat cgtagcgaac taccggcggc tcgtcggagt
    36901 cagtgaccgc gcgtttgatt ttcttcacta ccgatttcgg atcttccagc aggccgataa
    36961 cgttattgcg gttatcgtca gacttggaca tcttcttggt cggctccagc agcgacatta
    37021 cgcgcgcgcc agatttcgga ataaacggct ctggcacctt aaagatatcg ccgtacagcg
    37081 cgttgaaacg ctgggcgata tcgcggctca gttccaggtg ctgtttctgg tcttcaccca
    37141 ccggcaccag attagtttga tacagcagga tgtccgctgc catcagcacc ggatagtcaa
    37201 acagaccagc gttgatgttc tcggcataac gcgcagattt atctttaaac tgtgtcatgc
    37261 ggctcagttc gccgaagtag gtatagcagt tcaatgccca gcctaactgc gcatgttccg
    37321 gcacgtggga ctgaacaaaa atggtgcttt tctcaggatc gataccacaa gccagataca
    37381 acgccagcgt atccagcgtc gctttacgca gcttctgtgc atcctggcgc acggtgatcg
    37441 cgtgttggtc aacgatacag taaatgcaat ggtagtcatc ctgcatgttt acccactgac
    37501 gcagcgcacc catgtagtta ccaatggtca attcacctga gggctgtgcg ccactaaaaa
    37561 cgatgggctt agtcattttt cgattcctga ttttcgctat gcggaagccc taatgcgggc
    37621 agaaggtcat ttagagactg ataaattaca tcaggctggc tgagatcgat agcctcgccg
    37681 tagttatatc cgtaggttaa gccaactgat gggcaacctg ccgcttttgc tgcctgaata
    37741 tcattgcgtg agtcgccaac aaacagcatc tgttgtggag caatgcccat ccgttcagcc
    37801 accagtaaca gcgggtccgg atgcggtttt ttgttttgca catcatcgcc gccgataacc
    37861 acgctgaagt atttggcgat atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt
    37921 ttgttggtga ccaggcctag cggcaggcct ttagcctgca acgcgcccaa cgtatcggca
    37981 acgtgcggga acaaaaacgt cccctcttcg gcaacctcgc catagtagcg atcgaacagt
    38041 ttacgcagaa tacgtacctg ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc
    38101 attgttttac gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga
    38161 atatctgcgc cgttaccaat ccaggtaata acgcgttctt cacctgcgac aggcaactcc
    38221 agcgcataca gcgccatatc taccgcagca gcaagaccgg gggcactgtc aaccagcgta
    38281 ccgtcgagat caaaagcgac gccgcgaata tcttcaaact tattcatgac ttacctttgc
    38341 cagttcactg cgcatttcat caatgacttt tttgtagtct ggctggtcga agattgccga
    38401 accagcaaca aacatatccg cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac
    38461 gccaccgtcc acttccagtc gaatgtcaaa gccagactcg tcgatacgac gacgtacttc
    38521 gcgcagttta tccagtgttt gaggaatgaa agactgaccg ccgaaaccag gattgacgga
    38581 catcagcagg atcacatcca gcttatccat cacgtaatcc agataactca gaggtgtcgc
    38641 cgggttaaat accaaacccg ctttacagcc attttcttta atcagttgca gcgtgcggtc
    38701 aacatgctcg gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc
    38761 aggcacaatg cgatcgacgg gtttcaccat cagatgtacg tcgatagggg cggtaatgcc
    38821 gtagttacgc aaggatttca gcaccattgg cccaatcgtc agattgggaa catagtggtt
    38881 atccatgacg tcaaaatgca cgacatcagc gccagctgcc agggcttttg cggtatcttc
    38941 acccaggcgg gcaaaatcag ccgacagaat tgagggggca atcaaatact gtttcatccg
    39001 cttctccttg agaattattt tttcgcgggt gaaacgactc ctggtttgta caaagccagc
    39061 agttcgtcca cctttttacg tgtgccgccg ttgctgctta tactgcgtcg aactttgacg
    39121 acatgcaatt ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg attggagatc
    39181 agcaccggaa tatggcgatc aaccagacct tcagcgatct ccgccagatg cgcttgttgt
    39241 tcaagcgtaa aactgtttgt gtgatacgcc gtaaagttgg cagtcgcaga cagcggcgca
    39301 taaggcggat cgcaatagac gacagatgca tcatctgcgc gcgccatgct atcggcgtaa
    39361 gactcacaat agaaaaaggc attctgcgct ttttcagcga agtgatacaa ctctgcttcc
    39421 gggaaatagg gttttttgta gcggccaaac ggcacgttaa actcaccgcg cagattgtaa
    39481 cgacacaggc cgttgtaacc gtagcggttc aaatataaaa acagtaccgc ccgacggaac
    39541 ggatcctggc ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt
    39601 tcgggaacaa acagctcgcg tgcggcctgt acgtactcat cagtacgcat cttcacaatg
    39661 ttatagagac tgatcaggtc gctattgata tcggcaagga tataacgaga aaagtcggtg
    39721 ttgagaaaca ccgacccggc acctacaaaa ggctcaacca gacattcgcc cttgggcaaa
    39781 tgccgtttaa tatcatcaag caggggatac ttgccccctg cccacttcaa aaaagcgcga
    39841 tttttcttca tgctgactaa ctaattacac cttctccggc tgtggagaaa gctccgacag
    39901 catccagcgc ttcaggcaac gcccgcaatg ataaaccatt gcgggagata accttgatta
    39961 cttcagatcg gcctgtacct gacgcagcgg tttcgcccac gggtttttgg cctggacatc
    40021 tgctggcaat gtagataccg cttttttcgc ctcttctttc gaagcataca cgccagaaac
    40081 caggacatac cacggctgac cattacgcgt cgtttcatag acaacgtagt ttttcagatt
    40141 ctctttcttc gcccaaccgt tcaggttgtc gtagttagag gaactgctca gctgcagagt
    40201 gtaatggctg gacggtgccg atttcaacga accaacatta cctgcggtct tccctccagc
    40261 ggctggtgtt gccgtggttt gcgccgggga tgccgtctgt actggagccg tagtcgccgt
    40321 ctcttttggt gctggcgtag aagtcgcagc cggtgccttt gtgctcgcca caggggcagc
    40381 tggttcagtt cgcttcggcg tctgtgcgac tggcttcggt tccgttttca cggttgcttg
    40441 cggttttttc ggttcaatca ccgcctgctg acgagccggg cgcgtagtgg ccggacgttc
    40501 agcggtttgc gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag gcgcaaccgt
    40561 tgcgggttcg gtcggcaatg tagaattgac cgccacattg ttcagctgtt gctgattttg
    40621 tggctgggtc agcgcattgt tcaggtcacc ctgcacttca acacgttgtt gaccatccgt
    40681 tgccgccggg atttgccctt gagtcggcgt agaagagatc ggcggcagag aaacatcctg
    40741 ctgagtattt tccgcagacg tggttcccgg cgctggctgc acgccattgg cctgatcggt
    40801 cgcattgcct gcaagatcaa tactcttctc gccagacgcg gtttgatcgc tggaagaggt
    40861 cgagggggct tttagcgcag aaccgatacc gatgatcaac agcagtagaa ccagaatgcc
    40921 gacacccatc atcatatact gacgagaagc gggtttactg gctgcttttt tgcgcttacg
    40981 cggacgacgc tctacgcgct cttcatccac ggtttcatct tcggattcgt caagttcttc
    41041 ttcgatttcc ggttcctcat tacgcgcttt ttgcgcacga gtcggacggc gatcgtcagt
    41101 gtcatcaagt tcaatatcat caaaattgat ctgcggttcg ccacgttcag tacgctcaga
    41161 acgttcagaa gattgacgag aacgaccagt acgacgatcg ctgggatcgg gtttcagctc
    41221 gtcttctggt ttgaattcat ccatttaaca ccccactaaa aggttaatgc ttaccacgtt
    41281 gcaattaacc tgaagctaat agaccgcttg ataagcggcc tgacctttct tgttgttacg
    41341 ctgattgaca atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt
    41401 cactcttacc aattgccaac ggaagaatta agcgcatctc tcccgcaagg actttcttgt
    41461 cacgcagcat atgcggtaaa tacgcctgtg cggacatttc gcgcggccca ttgaccggta
    41521 acccagcccg tgtgagcagg gttataatac gctgcgtttc ggcagaacta aactgcccga
    41581 gacgttccga cgtccgcgcc gccatcacca tacccgcagc gaccgcttca ccatgtaacc
    41641 aattgccata ccccatttca gcttcaatgg catgaccaaa ggtgtgtccc agattcagta
    41701 aagcacgtaa cccggtttcg cgctcgtcgg cggcgacaac ttctgctttc agctcacaac
    41761 aacggcgaat acagtacgcc attgccggac cgtccagacg caacaacgca tccagattct
    41821 cttccagcca gttaaaaaac gcaccgtcaa gaataatgcc gtatttgatg acttctgcca
    41881 gccccgacgc taactcacgc gggggaagcg ttttcagaca gtcgagatcc accaccactg
    41941 aagcgggctg gtagaacgcg ccaatcatgt ttttaccgag gggatggttg accgcagttt
    42001 tgccgccaac ggaggagtcg acctgagaca gtaacgtcgt cgggacttga atgaaacgta
    42061 caccgcgctg ataactcgcc gccgcgaagc cggtcagatc acccactacg ccgccgccaa
    42121 gcgccaccag agtagtatcg cgaccatgcg gcttttgtaa caatgccgta aagacggtat
    42181 tgagtacagc caggctttta tactgctcgc cgtcagggag gataacgcta tcgacgttaa
    42241 cacccgcctg ttcaagtacg ccgcggacct tatcgagata cagaggagcc agggtttcgt
    42301 tggtgaccaa catgacctgc tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa
    42361 acaaaccaga tgcgatggta attgggtaac tacgttcccc gagagtaacg acaatcctct
    42421 ccataacgcg acatccacct taattactgt acccgcagac gagtgtatat aaagccagaa
    42481 ttagttgctt tccagcatgt gaataatctg gtttgcaacc actttagcgc tttgatcatc
    42541 agtacgaatg gtcacgtcgg caatctcttc atacagcgga ttgcgttcat tggccaacgc
    42601 ttccagaact tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg
    42661 cgtgcgtgca agttgctttt cgatggtcgt ttcaagataa acgacaacgc cacgagcgga
    42721 aagacggtta cgcgtttcac gggatttcac agagccgccg ccagtagcca gcacaatacc
    42781 ctgtttctcg gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc
    42841 ttctaaatcg aaaacccagc ccacatcagc tccggttcgt ttctcaatct cttgatcgga
    42901 atcgtaaaat tccatattga gttgttgagc taactggcgc ccaatagtgc tttttccggc
    42961 acccataggc ccaaccagaa agatattgcg tttctctgcc attttttcgg tactactaag
    43021 actattcgtt aatgataaac ccgcttcgct cagagagcgc cgcaggacat gaactgaaac
    43081 ctcataagat attgcgagag tcagactgaa aattatctca atactccagc gggtttggca
    43141 actgaataaa tcacccatcc ctctgcatat ctggtcgctg caagcgcgct gccttgctac
    43201 caccgctctg gcgataaatc accgggtaag attagcgtaa aaaagacagc aaaatgccgc
    43261 ctgaatgata aatcatcatc atggaacgca ggcagcgaag agcaccaact ctcaaatcgg
    43321 tactccttgt atgctaaata cctgcgcgcg tcaaatagat gaaacacgtt cagcgtaaaa
    43381 acattaccgc ttttacggct gtttactcac tggaaaccag tcgtggcgtg ataaacacca
    43441 ctaactcgcg tcgttcatct tcttttccgt catgacgaaa taattgcccg aaccagggaa
    43501 tgtcgccaag caacggtacg ctatcctgac ccgatttatt tttacgggta aaaatgccgc
    43561 ccagcgccaa cgtttctccg cttttgacct cgacctgcgt ttcgatctcc tgcttatcaa
    43621 tcgccagtac ttcgccatct gcctgctgta gcacctgccc cggaacgttc tggctgatgt
    43681 gtaatttcag ccggatgcga cctttttgta acaccgtggg cgtgacttcc atccccagga
    43741 cggcctcttt aaattccacc gacgtcgcgc cactttcccc gctggaaacc tgatatggaa
    43801 tttcgctccc ctgtttaatg ctggcaggct gaagatgtga ggccagcaga cgcggactgg
    43861 cgataatatc cagctgctgt ttttgttcga gcgcggaaag ctcaagatcc agtaaacgtc
    43921 cgttgatgcg tccaatgtta aaaccgatat gcgttgtcgc cgtcgctacg gagaggtcgc
    43981 tgccaagcgt ggtgacttgc ccaacgccac caccttgttg cgcatcggcc aacgtccatt
    44041 tcacacctaa ctcacgcaaa cttttttcat taatggtgac aatatgcgcc gacagctcaa
    44101 cctgcccgac cggcagatcc atttgcgata cccactgttc aagcgtgctt aacgccgttt
    44161 tgttatctcg cagcaaaagg cgattggtgc gtttatcgac ggtcatactc cctttggcac
    44221 tcagtagctt ctcccccgct ttcgccagct ctccggcgtc ggcgtattgc agagtaatat
    44281 tgcgattttc cagcggcaga tttgcctgcg cccgcgtctg ctccgcctcc tggcgggcga
    44341 tattgtcatt ctgccaggca acggaatgca ccgagagaat gttgccctcc tggcgcgtta
    44401 tcaatccggc gcttttcact acagtttgta gtgcctgctt ccagggaacg tcagttaaat
    44461 gtaacgacac cgtaccgctg acgtctggcg aaaccaccag gttcaacttc tcctgttcag
    44521 ccagcgcctg caacacctga gctaccggaa cgtcatccac catcagcgtc actttttgcg
    44581 gctttgccgc ctgtacgccg ggtataagca tcaacagtag tgcggctatc cattgcttca
    44641 tttgtatctc cttgccgttg ccacaaccag tgtggcggtt cgcagtttgt cccggttccc
    44701 agcgttagtg cgtctggcgt cagctgtaag atcgtccagc cgttttccag cacatcgttt
    44761 tgctgcaccc gtcgccattt tttttgccca tcttttatta caccgatgat gcgctcgcct
    44821 cgccctacca tcccctgata gcgccactgg ctaagttcgc taatccggca tagatcttcc
    44881 ggcggtttaa aagggtcacg cataccggtt aaaaggcaca atgcaatgcc tgctaacaac
    44941 cagcgtttaa ccctcatgcg gcgtctccag ttgtagcgtg aacaaaaggt catcaccttc
    45001 cacgcttaac aaaaaacggc tcacgctgac gttgcgctct gccagccgtg taaatgccga
    45061 cggcactgct tcccacagcg ttttcaacgc caactcacct ccctgcgcgg atggatgcca
    45121 gtaaaccagt tgcgcgccgg ataactgaaa atccagtggc gaaaagggca gcgttttttc
    45181 ctcgctgaag ggagtggtgt ctaccaggcg atacagtgca gcccactgct ggtgatgatt
    45241 tgcccgtagt cgaattaatg cgtcgctctc ttcatggtgt gtcaatgaca gaaaaatgag
    45301 cgtaactaac accagcaacc agactgccca gcaaaactgg cggaggcgga gtgatgtggc
    45361 gaaccaccag tcaaagaaca tgttcatcgc taaccttcct tgttaactga tactcaaatt
    45421 gccagcgtcc ctgcgcatcc tgctgcgtgg ccccccgctg attgagatga aaagaagcat
    45481 cctggcggag tgacgtttcc agcgcgttta acgcggcaat gcttgttgtc agccccttga
    45541 tctccagcgt tccctgctgc cagcttatcg ttgtcagcca ggcatgttcg ggtaaaagcg
    45601 ccgccagtgc ttccagagca gattgccagt cgcgggtaaa ttgtcgttgg cgctgccgtt
    45661 gcaaacgctg ttcgcgtaac tgctggcgtt ccagcaaacg tggtttcgtt atctgtaagc
    45721 tgcgggcgag ttgttgttcc gcctgaagca aaacggcgtt tacgcgtgct tcggcattgc
    45781 ttgtcagacg cagtattagc gttatcccga tggccagcag cagaggcgca acgaacatca
    45841 gcaaccagaa acgaagaaaa gcagtccggc gttgctgtcg ccagggcaaa aaattaattg
    45901 gcgggttcat cagtacacct tcccaagcgc cagccccagc gcgatggcaa agtctccacc
    45961 tggtggcggt agcggcggct gacgaacaga aaccgcctcc caggggtcaa atccgccttc
    46021 gccacatagc gcgacgcttt cgggatcaac agataacgct gccgccagct ccttcgcgct
    46081 agtcatcccc accgccagtt tgcgccccca gctatagcgc gtcgcccaca gccactgttc
    46141 gttatcacgc caggccagac attgctgatg agaaggtaaa aaaggcagga atcgctgtaa
    46201 tgcactggca tccggagtga tcgcgctaac atgaacacgc aacctttctg ccagagtaag
    46261 cagcgttgcc agctctttgc tttgcgcggc agtcacgtta taggcgggac tgagtgagtc
    46321 ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc agttcacggg ccatcgtccc
    46381 ggacagccag gccatttgct cccgctcacc gagggacatc gacgggcgtg gaaatgaccg
    46441 ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga tgacgctgcg gcagttcacg
    46501 actccacggt aacaacgtgt tagccagccg ctgcacatca acaatccgcc catctttgat
    46561 aatgtcgttc tccagtggca accgccacca gcggtgcaat aagcattctt ttgcgctccg
    46621 cacgatagca accgctaccg cttcttgctg ttgtaaatgc aaaccaattt gccagttctt
    46681 aaatgccatt gtgatgatct ccttatcacc cgtcactctg acgggtatat caatgcgtct
    46741 ggcttgcctt tatactaccg cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga
    46801 aatttccagt gaagttcgta aagtattttt tgatccttgc agtctgttgc attctgctgg
    46861 gagcaggctc gatttatggc ctataccgct acatcgagcc acaactgccg gatgtggcga
    46921 cattaaaaga tgttcgcctg caaattccga tgcagattta cagcgccgat ggcgagctga
    46981 ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt ggatcaaatc ccgccggaga
    47041 tggtgaaagc ctttatcgcg acggaagaca gccgcttcta cgagcatcac ggcgttgacc
    47101 cggtggggat cttccgtgcg gcaagcgtgg cgctgttctc cggtcacgcg tcacaagggg
    47161 cgagtaccat tacccagcag ctggcgagaa acttcttcct cagtccagaa cgcacgctga
    47221 tgcgcaagat taaggaagtc ttcctcgcga ttcgcattga acagttgctg acgaaagacg
    47281 agatcctcga gctttatctg aacaaaattt acctcggtta ccgcgcctat ggtgtcggtg
    47341 ctgcggcaca agtctatttc ggtaaaactg tcgaccaact gacgttgaac gaaatggcgg
    47401 tgatagccgg gctgccgaaa gcgccttcca ccttcaaccc gctctactca atggatcgtg
    47461 ccgtcgcgcg gcgtaacgtc gtgctgtcgc ggatgctgga tgaagggtat atcacccaac
    47521 aacagttcga tcagacacgc actgaggcga ttaacgctaa ctatcacgcg ccggagattg
    47581 ctttctctgc gccatacctg agcgaaatgg tgcgccagga gatgtataac cgttatggcg
    47641 aaagtgccta tgaagacggt tatcgcattt acaccaccat cacccgcaaa gtgcagcagg
    47701 ccgcacagca ggcggtacgt aataacgtgc tggactacga catgcgccac ggctatcgcg
    47761 gcccggcgaa tgtgctgtgg aaagtgggcg aatcggcgtg ggacaacaac aaaattaccg
    47821 atacgctgaa ggcgctgccg acctatggtc cgctgctgcc tgccgcagtc accagcgcca
    47881 atcctcagga agcaacagcg atgctggcag acgggtcgac cgtcgtattg agtatggaag
    47941 gtgttcgctg ggcgcgtcct taccgttcgg atactcagca gggaccgacg ccacgtaaag
    48001 tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg tcaagttggc gatgcatggt
    48061 ggctggcgca agtgccggaa gtgaactcgg cgctggtgtc gatcaatccg caaaacggtg
    48121 ccgttatggc gctggtcggt ggctttgatt tcaatcagag caagtttaac cgcgccaccc
    48181 aggcactgcg tcaggtgggt tccaacatca aaccgttcct ctacaccgcg gcgatggata
    48241 aaggtctgac gctggcaagt atgttgaacg atgtgccaat ttcccgctgg gatgcaggtg
    48301 ccggttctga ctggcagccg aagaactcac caccgcagta tgccggtcca attcgcttac
    48361 gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg
    48421 tcgactacgc tgcagaatat ctgcaacgct tcggcttccc ggcacaaaac attgtccaca
    48481 ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg
    48541 cggtcatggc gaacggcggc ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg
    48601 atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc
    48661 cggtgattta cggtgatacg cagaaatcga acgtgctgga aaataacgat gttgaagatg
    48721 tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa
    48781 atcaggcgtt agtggcgaag actggcgcgc aggagtacgc accgcacgtc atcaacacac
    48841 cgctggcatt cctgattaag agtgccttga acaccaatat ttttggtgaa ccaggctggc
    48901 agggtactgg ctggcgagca ggtcgtgatt tacaacggca tgacataggc ggaaaaacgg
    48961 gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta cggtccgggc gttgtgacct
    49021 cggtctggat tggctttgat gatcaccgtc gtaatctcgg tcatacaacg gcttccggag
    49081 cgattaaaga tcagatctca ggttacgaag gcggtgccaa gagtgcccag cctgcatggg
    49141 acgcttatat gaaagccgtt cttgaaggtg tgccggagca gccgctgacg ccgccaccgg
    49201 gtattgtgac ggtgaatatc gatcgcagca ccgggcaatt agctaatggt ggcaacagcc
    49261 gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa
    49321 cgaccattat cgataatggc gaggcacagg aattgttctg attaaaaagg cgcttcagcg
    49381 cctttttaag tttgctgaca aagtgcactt tgtttatacc ggatgcgggt gaacgcgtta
    49441 tccggccaac aaaaaattga aaattcaata aattgcagga acttcgtaag cctgataagc
    49501 attatgcatc aggcatcagg caaacttcac gcatttacac tcgcccctgc cctttcaacc
    49561 attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca ggggcttcca
    49621 gcaaatccat catatgcgcc agcggccagc gcacctgtgg taacggctct ggctcatcgc
    49681 cttccagtga ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaagt
    49741 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatccaacct
    49801 cttcttttag ctcgcggtta gcggcttcaa agacgctttc acccggatca attaatcctt
    49861 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    49921 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    49981 aaacacgccg cacgccattg ctgaactcca gatccacgct ttcgacggta aacagtcggg
    50041 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg
    50101 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    50161 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    50221 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    50281 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    50341 agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga cagactaagt
    50401 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    50461 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    50521 ccttcgtgga tgcgcaaacg cgtaaactca catctgaaga acgtagcgcc gttgaaaatt
    50581 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagcc agcgcggcac
    50641 cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca
    50701 cgcgttatgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    50761 aagtccacct gccccctttc tgggaacagt acatcaacga tgagaatacc gttgaactga
    50821 ttcataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca
    50881 tgcaggaaac tcgcggctat gccttgcaac ctgttccgtc aacgcaggcg tcgattcgcg
    50941 gggaagagag cgagcaaata gagctgctca atattcgcaa agaaacgcac gaagagtatg
    51001 cgctgagtcg tccgcgcggg ctgcgcgaag cgttgctgat tgtcgcctcc ttcatgatgt
    51061 tctttttctg cctgattacc ccggatgtct ttgttccgtg gctggcaggc ggcgcgttac
    51121 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcggg
    51181 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    51241 aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca cactggcagc
    51301 catacattgc tcaggatctt ggtcaacaaa ccgatatcga tatctatctc gaccgccacg
    51361 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    51421 actggctgcg cagtacgatt attgctgcgg gctcgctatt ggtgctgttt atgctgttat
    51481 tctggatccc gctggatatg ccgctgaaat tcaccctctc gtggatgaaa ggcgcgcaga
    51541 ccattgaagc caccagcgtg aaacaactgg ctgacgctgg cgtgcgggtt ggcgatacat
    51601 tgcgtattag cggtacggga atgtgtaata ttcgcacttc aggtacctgg agcgcgaaaa
    51661 ccaactcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac gcccgctcat
    51721 tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgaccgaa gcagttaatc
    51781 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta cgttcagcaa
    51841 ttcaaaaatc cggtatggta ttgcttgatg attttggcga cattgttctg aagacagcag
    51901 atctgtgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    51961 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaaactc gatggcgtga
    52021 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtgacc acctctaccg
    52081 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    52141 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    52201 catcacttta cgactatccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    52261 tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc actgacgcca
    52321 atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    52381 tcagcaccac gctgctgcta ctgacgatgc tgggtagcgc catttataat ggcgtacagg
    52441 cctggcgtcg ttaccagcgc catcgtactc gcatgatgaa gattcaggcc tattatgaaa
    52501 gctgcctgaa cccgcagctg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    52561 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    52621 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctt
    52681 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    52741 aacggagtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    52801 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    52861 gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt
    52921 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca tccgcacaac
    52981 ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt actttccacc
    53041 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    53101 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    53161 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgactccgg gattgccgag
    53221 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    53281 tgaaggagat gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg
    53341 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt
    53401 gcattacaac gggcagcgca gcaagccaag caaaatcgtc gagctgaatg ccacgctcac
    53461 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    53521 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    53581 aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcatccgg accgacgccc
    53641 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    53701 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    53761 ctttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    53821 tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    53881 gttaaccgct acgttgaagt ttgatggtga tatcaccgta cagctgcaag gcgacggtcc
    53941 gatgaatcta gcggttatta acggtaacaa caaccagcag atgcgcggtg tggcgcgcgt
    54001 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    54061 gatcaccatc accccgagcg aaggcgaacg ctatcagggc gtggttggtc tggaaggtga
    54121 taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct
    54181 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggaatgt tgttgcaggt
    54241 aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcaacgc taaccgaaac
    54301 catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    54361 cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    54421 tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagtcgata gcatcctggc
    54481 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    54541 gatggatatt gccgagatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    54601 agtatgtccg gcagagagga ctctgccgga ttttacgact aatacacctg atgcgctttg
    54661 tcactcattc agattcacat ccagcctttc accatccact acaagatcgc gccacgcttt
    54721 aggcctcgtc cccagttttc catccttcgc agctgtgagt aatttttctt tcgttatttg
    54781 gcgcggaagt aaactcatat caagcgcgtc ccattctttt ctggctgagt caaagccata
    54841 gacaatgact ctgtcatcat taaaagcaaa caggatccgc tccgcctgac catcactatt
    54901 cagatcttgc tcgaccagta cgcaggcgtc tttttcaata caggtcatca cgttatagcg
    54961 atcctggatt aaggccgacc agaatgtcgc atccggttta ccagaacctg gggcaattaa
    55021 cacattatcg gctaatactt tttccgatac ctgttcctga agatgttgct ctccatctaa
    55081 cgccatcagc agatcccgcg cgcgtttcgg gtctttcata tactcggcat cgcttttcag
    55141 cgactcaagc gccgcacgtc catagcgacc gctctgttcg agcatataga gacttacctg
    55201 gtctggcgtg tttttgccgc tctgataacg cgccatatga ctgttcacgc taatgcgcat
    55261 actgtccagc accggagaat taagaagcac cagtattacc aacaccaata acgaaaccgc
    55321 aaggttcact ttgccctgaa gaacaagggg gttttgccct ttacgccaga cgatactgac
    55381 aaaatacccc agcgaccata ccagtaacac cagcaccgcc agcgcgccct gcaagcggtc
    55441 gacagtccag ccgtactgag cgaccggcat ccataacgcc caggcggcga cgaacacata
    55501 cagcggagca accagcaaag cggttttaat caggcaacgc aacggccccg tccagggaag
    55561 tgacgctttt tgcggatcgc ggacaatagc cattaagatc aattgcaaaa aggccagcgt
    55621 taacagcaac ccagcggcgg agatgtggcg agaaatcgcg ctcaggcccg taaacggcag
    55681 ggtgataata aacatcaggg ttagcaatga tactaacggc agcaaccccg tggcgattaa
    55741 cgtgaacaac ttttgaatag agtcgattaa acgtgactgt gttcgcgcga ggatcaccgc
    55801 cagcgcggta gccagaccta acgtgagata aataaaccag tcggttgcaa aaaagagcgt
    55861 tttgaaaaac gtgatgccaa caagtttaaa caactcactc cacagtaaaa gcaccagcca
    55921 cgtcaggcca ttggcgagaa aaatcaccag taatataagt acgttatgcc atacagactg
    55981 gtaaaagtag cgatagcggc tactgtcatt gcggatgcga aggctttgtt gtatccacgg
    56041 tagcaacaac attgccatca gcagcagata gcagccaaaa tcccaaagcg ccttttcagc
    56101 tctccagggg gtcatgccat cagtttgcca cttcagccag ccgctcattc ccagcgtggc
    56161 aataaacacc agcgccagcc atccccaaag gcgcttctgt ttaaaggaga tcacggagaa
    56221 gagtaaaacg gatgaaaacg cgacggtcac aggcacgcca taaacaatcc aactgagatt
    56281 ttttccggca agccaggcga tcagcaggta gcaaaccagc ccctgaagta atccggtagc
    56341 aatcatcccc cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa
    56401 tcaaaaggtg ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa
    56461 tcaaaaaaag ttagcgtggt gaatcgatac tttaccgatt gaatttgcat caatttcatt
    56521 catgaatgcg attccactca caatattccc gccatataaa ccaaaattta accttttgag
    56581 aacattttcc acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa
    56641 tcacggagtt ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg
    56701 cggttaacac ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt
    56761 gaaatgcgcg ttaacaatgg tttgaccccg caagaactcg aggcttatgg tatcagtgac
    56821 gtacatgata tcgtttacaa cccaagctac gatctgctgt atcaggaaga gctggacccg
    56881 agcctgaccg gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc
    56941 gggatcttca ccggtcgttc accaaaagat aagtatatcg ttcgtgacga taccactcgc
    57001 gatactttct ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg
    57061 gaaacctggc agcatctgaa aggcctggtg accaaacaac tttccggcaa acgtctgttc
    57121 gttgtcgacg ccttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc
    57181 gaagtggcct ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag
    57241 ctggcaggtt tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag
    57301 tggaaagaac agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg
    57361 cagctgattg gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcaatgatg
    57421 aactacctgc tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag
    57481 aaaggcgatg ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc
    57541 gacccgaaac gtcgcctgat tggcgatgac gagcacggct gggacgatga cggcgtgttt
    57601 aactttgaag gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa
    57661 atctacaacg ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc
    57721 actatcgact ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccgatctat
    57781 cacatcgaaa acatcgttaa gccggtgtcc aaagcgggcc acgcaactaa agttatcttc
    57841 ctgaccgcag acgcctttgg tgtgctgccg ccggtttctc gtctgactgc cgatcaaacc
    57901 cagtaccact tcctctctgg cttcaccgcc aaactggctg gtacagagcg cggcatcacc
    57961 gaaccgacgc caactttctc cgcctgcttc ggcgcggcat tcctgtcgct gcacccgact
    58021 cagtacgcag aagtgctggt gaaacgtatg caggcggcag gcgcgcaggc gtatctggtt
    58081 aacaccggct ggaacggcac tggcaaacgt atctccatta aagatacccg cgccattatc
    58141 gacgccatcc tcaacggttc gctggataac gcagaaacct tcactctgcc gatgtttaac
    58201 ctggcgatcc caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc
    58261 tacgcttctc cggaacagtg gcaggaaaaa gccgaaacgc tggcaaaact gtttatcgac
    58321 aacttcgata aatacaccga caccccagcg ggtgccgcgc tggtagcggc tggtccgaag
    58381 ctgtaatgat ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag
    58441 gcaccttcgc ctcccgtttt atttaccctt cttttgtcgc accctgcgcc cgcgttaccg
    58501 gcactggcag ccaggcgcga atggaaagcc cgccccgttc gctggtgcca agctccagca
    58561 tcccgttatg gttatccacg atacgctgca caatcgccag ccctaatccc gtgccgctaa
    58621 tagtgcgcgc actgtcgcca cgaacaaacg gctggaacag gtgcttacgt tgttccggcg
    58681 caatacccgg accgtcatct tctacctgga accaggcgcg attcggctcc gtgccgctgc
    58741 tgactttgat ccagccattg ccgtaacggg cggcgttgac caccatattc gccaccgcac
    58801 gtttgatcga cagcgggtgc attttcacct caatgctgcc ggggtaaagc gcggtttcaa
    58861 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagcactgcg ttgagatccg
    58921 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    58981 cgttgcactc ttcgatatct ttattgatcg attcggccag gtagccatcc tgctcgctca
    59041 tcatctcggt cgccaggcga atgcgcgtca gcggcgtacg cagatcatga cttacccccg
    59101 ccatcagcag cgtgcgatca tccgccagtt gcttaacacc agccgccata tggttaaagg
    59161 cgcgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc
    59221 ctttaccgac ctgcagagca gcatgttcga gatcgaccaa cggtcggttc tggatacgga
    59281 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    59341 gagaaaaatc accctgatga atttcggtca gcggcacgcg cacccagata ttgggcgaca
    59401 gccaggtttt cagccagaca acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    59461 ggccgcccag ttgctgcgcc atctgatggc ttaagaattc atagtgttgt gcccaacgca
    59521 gacctgcctc ttcggcagct tcgttggaat agagagagat ccccagctca cggtagatct
    59581 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    59641 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    59701 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct gacgaacagc aaggtgacga
    59761 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    59821 agaaccgtcc ggtacaaaga cgtagcccag accccagacg gtctgaatgt aacgcggatg
    59881 cgccggatct tcttccacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    59941 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    60001 acgcggatgg ctgaccagcg ccttaagtac cgcaaactca ccactggtga gcggcatcgg
    60061 ctcgtcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    60121 taccgcctct tcctgtgacg gcgcgcccgg cagttcgttg gcctgacgac gcagcaccgc
    60181 acgaatgcgg gccagcaatt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    60241 aatctccagg ccaacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    60301 catcgggttg ctctggctac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    60361 catcaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    60421 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    60481 gcgcaggcgc atgtcgtcat caaccaccag aatcttgtag ttctcttgca ttgtttgtac
    60541 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    60601 agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    60661 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    60721 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    60781 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    60841 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    60901 atcagtataa taaaaagcgc ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    60961 gcatggaaaa tctcaaaatc gtcgattact ccccgcagca ggaaggcaaa gtcttttttg
    61021 gtgcgtgggt ggagattgaa aacgacgatg gtgtgactca ccgtttccgt attgtcggct
    61081 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    61141 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgagct
    61201 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    61261 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    61321 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    61381 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    61441 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    61501 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaagtcta
    61561 tttccgagca aggcaaactc accgatgatc tggcgaatgc catcaacgcc accctaagca
    61621 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgtgggcaaa
    61681 tcgccattga agcagggctt gagccgttgg ccgatctgct gtggagcgat ccgtcacaca
    61741 cgccagaagt cgccgctgca caatatattg atgccgataa aggcgtggca gataccaaag
    61801 ccgcgctgga cggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    61861 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtctcaacc gtggtgaatg
    61921 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgctgtcca
    61981 cggtgccttc tcaccgcgcg ctggcgatgt tccgtggtcg taacgaaggc atactccagc
    62041 tttcgctgaa tgccgatccg cagttcgaag agccgccgaa agagagctat tgcgagcaaa
    62101 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag
    62161 gtgtggtgag ctggacgtgg cgcatcaagg tgttgatgca tctggaaact gaactgatgg
    62221 gcaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    62281 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    62341 gtaccggggt aaaagtggcg gtagtcgatg caactggcaa actggtggcg accgatacca
    62401 tttatccgca taccgggcag gccgcaaaag cagcgatgac cgttgctgcg ctgtgtgaaa
    62461 agcataacgt tgaactggtg gcgattggca acggtacagc ttcccgcgaa accgaacgtt
    62521 tctatctcga cgtgcagaag cagttcccga aagtgaccgc gcagaaagtg atcgtcagcg
    62581 aagctggcgc gtcggtttac tcggcttccg agctggcagc acaggaattc ccggatctcg
    62641 acgtttcgct gcgtggcgcg gtgtctatcg cccgtcgttt gcaggatccg ctggcggagc
    62701 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    62761 cgcagctggc acgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    62821 atctcaacac cgcttctgtt cctctattaa cccgtgtggc gggcctgacg cgcatgatgg
    62881 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgt
    62941 taaaagtgag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ttgcgcatta
    63001 accacggtga taacccgctg gacgcttcta ccgttcaccc ggaagcctat ccggtggtgg
    63061 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    63121 tgcgtaacct gaaagcgtct gactttactg atgagaaatt cggtgtgccg acggtaaccg
    63181 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    63241 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggtg
    63301 cggtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    63361 tggttcacat ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    63421 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    63481 tgaccatgcg tctggacgag cagcctggcg aaaccaacgc ccgtcgcggt ggcggtaacg
    63541 atcgcccgca aaacaaccgc ccggcagcca aaccgcgtgg gcgtgaagcg cagcctgccg
    63601 gtaatagcgc gatgatggac gcgctggcgg cagcaatggg taaaaagcgt taaaccccct
    63661 gattggccta cggttcaaat tttcacgaaa tcgtaggcca gataaggcgt tcacgccgca
    63721 tctggcaata cacgccttgc ctgacataaa aatgccggag tatatctccg gcatttttat
    63781 tccacagcca accccacaat atactcccta atatttatca cttcattaac aactgagacc
    63841 ttaattaaac attaaccagc cctaataatt cgtcatgcga attatatttt cgctgcgata
    63901 taaccttgag ccatatcaac attgagtcag attattattc aaaccaacat tcacacacat
    63961 tttaagtatt gctgatagaa accattctca ttatcattgt actgttgatt atttaatctc
    64021 tccttcgttg gcaaatcatc tggtctcatg tcgctgtcaa acgccccatg aggtagttat
    64081 ccagttaatg agaaacaagt aggcacctat gcaatacact ccagatactg cgtggaaaat
    64141 tactggcttt tcccgtgaaa tcagcccggc atatcgccaa aaactgcttt ctcttggcat
    64201 gttacctggc tcctctttta atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat
    64261 cgaaacccgt cgtgtgagcc tggtattacg caaaaaagat ctggccttat tagaagtgga
    64321 agcggtttcc tgttaatacg gtgataacaa caatgaaaaa attaaccatt ggcttaattg
    64381 gtaatccaaa ttctggcaag acaacgttat ttaaccagct cactggcgca cgtcagcgtg
    64441 taggtaactg ggctggcgtt accgtcgaac gtaaagaagg gcaattctcc accaccgatc
    64501 atcaggtcac gctggtggac ctgcccggca cctattctct gaccaccatc tcatcgcaga
    64561 cctcgctcga tgagcaaatc gcctgtcact acattttgag tggcgacgca gacctgctga
    64621 ttaacgtggt ggatgcgtct aaccttgagc gtaacctgta cctgacgctg caactgttgg
    64681 aactcggcat tccatgcatc gtggcgctga acatgctcga cattgccgag aagcaaaata
    64741 ttcgcattga aattgatgct ctgtcggcgc gtcttggctg cccggtgatc ccgctggttt
    64801 caacccgtgg tcgcggtatt gaagcgctca agctggcgat tgatcgctat aaagctaacg
    64861 aaaatgtgga actggtgcat tacgctcagc cgctgctcaa cgaagcagat tcactggcaa
    64921 aagtgatgcc ttccgacatc ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg
    64981 aaggcgatat ctacagccgc gcctacgccg gtgaagcgtc gcagcatctg gatgccgccc
    65041 tcgcccgtct gcgtaatgag atggacgatc cggcgctgca cattgccgat gcgcgttacc
    65101 agtgcatttc tgccatctgt gatgtggtaa gcaacaccct gacggcagaa cccagccgtt
    65161 tcaccactgc ggtagataaa atcgtgctca accgtttcct cggtctgccg attttcctct
    65221 ttgtgatgta cctgatgttc ttgctggcta tcaacatcgg cggggcgtta cagccgctgt
    65281 ttgacgtcgg ctccgtagca ctctttgtgc atggtattca gtggattggc tacacgctcc
    65341 acttcccgga ctggctgact atcttcctcg cccaaggcct gggcggtggt attaacaccg
    65401 tgctgccact ggtgccgcag atcggcatga tgtacctgtt cctctccttc cttgaggact
    65461 ccggctatat ggcgcgtgcg gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc
    65521 cggggaaatc ctttgtaccg ctgatcgttg gtttcggttg taacgtaccg tcggtaatgg
    65581 gcgcacgtac gcttgatgca ccgcgtgaac gtctgatgac catcatgatg gcaccgttta
    65641 tgtcctgcgg cgcgcgtctg gctatcttcg cagtattcgc ggctgccttc ttcgggcaga
    65701 acggtgcgct ggcggtcttc tcgctgtata tgctgggtat cgtaatggcg gtgctgactg
    65761 gcctgatgct caagtacacc atcatgcgcg gtgaagcgac gccgtttgtc atggagctgc
    65821 cggtctacca tgtaccacac gttaaaagcc tgattatcca gacctggcag cgtctgaaag
    65881 gcttcgttct gcgtgctggt aaagtgatta tcatcgtcag cattttcctg agcgccttca
    65941 acagcttctc gctgagcgga aaaatcgtcg ataacatcaa cgactcggcg ctggcttccg
    66001 tcagccgggt gatcacccca gtcttcaagc cgattggcgt gcatgaagat aactggcagg
    66061 caacggttgg cctgtttaca ggcgcaatgg cgaaagaagt ggtggtgggc acgctcaaca
    66121 ccctctacac cgcagaaaat attcaggacg aagagttcaa tccggcagaa tttaacctcg
    66181 gtgaagagct gttcagcgcg gtagatgaaa cctggcagag cctgaaagac accttcagcc
    66241 ttagcgtact gatgaatccc attgaagcga gcaaaggcga cggcgaaatg gggaccgggg
    66301 cgatgggcgt gatggatcag aaattcggta gcgcagctgc cgcatacagc tacctgattt
    66361 tcgtcctgct gtatgtaccg tgcatctcgg tgatgggggc tatcgcccgt gaatcaagcc
    66421 gtggctggat gggcttctcc atcctgtggg ggctgaatat cgcttactca ctggcaacat
    66481 tgttctatca ggtcgccagc tacagccagc acccaactta cagcctggtg tgcattctgg
    66541 cggttatcct gtttaacatc gtggttatcg gtttgctgcg tcgcgcgcgt agccgggtgg
    66601 atatcgaact gctggcaacg cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg
    66661 gtgattgcca ttaatggctt cacttattca ggtgcgtgat ttgctggcgt tacggggccg
    66721 tatggaagcg gcccagataa gccagacatt gaacaccccg cagccaatga ttaacgccat
    66781 gctgcaacag ctggaaagca tgggcaaagc cgtgcggatt caggaagaac ctgacggctg
    66841 cctctctggc aattgtaaaa gctgcccgga aggcaaagcc tgcctgcgcg agtggtgggc
    66901 gctgcgttaa ccatactcca tcacctgttt tgaaaagccc ggtatgcgtt tgcatccggg
    66961 cttttttgcg tgcggctttc cataaaaatg caacccttgc agcgcgacgt aaattcctct
    67021 gaaagcgtct cgcagggatg aaaactcgct aatgcacagg tgtggagtgg cgcgtagagt
    67081 cgcggcattc aaacaacagg tgaaggaacg ccatgagcaa aaagcagagt tccaccccac
    67141 acgatgcgct gttcaaactc tttttacgcc aaccggagac ggcacgcgat tttcttgcgt
    67201 ttcatttacc ggcacccatt cacgcgctct gtgatatgaa aaccctcaag ctggagtcga
    67261 gcagctttat tgatgacgat ctgcgtgaaa gctattccga cgtgctgtgg tcggtgaaaa
    67321 cggaacaagg accaggatac atctattgtc tgattgaaca tcaaagcacc tcaaacaaac
    67381 tgatcgcatt tcgcatgatg cgctacgcca ttgccgcaat gcaaaatcac ctggatgccg
    67441 gatacaaaac cttgccgatg gtggtgccat tgttgtttta ccacggtatt gaaagcccct
    67501 atccctattc gctgtgttgg cttgattgtt tcgccgatcc caacctggca aggcagcttt
    67561 atgcctctgc atttccgctg attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc
    67621 accgacgtat ggcactgctg gagttaattc aaaaacatat tcgtcaacgc gacctgatgg
    67681 ggctggtaga gcaaatggcc tgcttattaa gtagtggata cgctaatgac agacaaatca
    67741 aagggctgtt taattacatc ttgcaaaccg gcgatgcggt acgttttaac gattttatcg
    67801 acggcgttgc cgaacattca ccgaaacaca aggagaactt aatgactatt gcggaaagat
    67861 tgcggcagga agggtttcag aaaggtatac gtaccaacgc cctaaatatt gccaaaacca
    67921 tgctggacgc cggagtttct cttgaggacg ttctgcgatt taccagcctg acggttgaag
    67981 accttgctga aataaatcat caataacgta aaggatggtt ttaatgacag atgcagaaag
    68041 agaatatctt attatttacc tgattggtta tttattgggt cgacgcctgg aagccctgga
    68101 tatcgccaaa agaatgctgt aatccggagt ccctcttgcg gacatcatgc gttttaccgg
    68161 ggtgtcagaa gaagagttgg ctgcggcgag tcagtaaagt tctgtctcgc catttcaaaa
    68221 gctgtctaca ccctctgctt caacgccacc agcaggtgat gaaactcgac cggatgcgaa
    68281 ataaacggcg catgggccgc tttggcgaaa atgtacgact cgctgtgcgg ccagagttta
    68341 tccagcatcg gcactacttt gcgcggcacc agaccatcaa gatagccata caatcgcaaa
    68401 aacggcatgg acacgttttg cagcggcaga cggagatcga ccgttttgag gatttccagc
    68461 ccaccattga gcacgtccac ctccggcatc ggtaacgcca gtacggtttt cttcaacgcc
    68521 cgcgcatcct ggcgcgccgt ttcagtaccc atggtttgta acgccaggaa ccgctccact
    68581 gtacgctgaa aatcatcact cagctgctgc tgaaatcccg ccagcacgtc cggttttatc
    68641 cccggccact cgtcacgagc actaaaacaa ggcgacgacg ccacggtgac cagcgcctga
    68701 acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct
    68761 aaccaaatgg ctttatctgg tgcctgtcgc agcacgactt cggccatatc agcaagtgat
    68821 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
    68881 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
    68941 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
    69001 tattgttctc tttttactta caaggatgaa catatgctaa cagtaccggg attatgctgg
    69061 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ctctcgcgcc
    69121 gcccgtacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctctcatctt
    69181 ccctgcggtc gctgcctgca aaaaccgctg ccctggcaaa gactggtcac ggtcgccgac
    69241 tatgcgccgc cattaagtcc gctcattcac cagcttaagt tttcccggcg cagtgaaatc
    69301 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggc
    69361 ttgcaactgc ctgatcgcat catcagcgtt ccgttatggc agcgccgtca ctggcgtcgg
    69421 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggctaca ctgccaatgg
    69481 gatagcgaag ccgtcacacg aacacgggcc accgcgaccc agcattttct taacgcccgg
    69541 ctacgcaaac acaacctgaa aaatgccttt cgccttgaat tgcctgtgca aggtcgccat
    69601 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
    69661 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
    69721 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
    69781 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaacc aggaagaagg
    69841 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggcgtttc
    69901 ttattgtccg ccggacgctg tggaagccac cgacactgcc ctgaaatttg acctgctgac
    69961 cgcgtatgtt gatgagttaa gcgctcctta cctggaagat gcagagatcg attttgttac
    70021 cgaccagttg ggttcgcagc tgacgctgaa agcgcctaac gccaaaatgc gtaaagtggc
    70081 tgacgatgcg ccgctgatgg agcgcgtgga gtatatgctg caatcacaga tcaacccaca
    70141 gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat
    70201 tttgcaattt ggcggtggct gtaacggttg ttccatggtc gacgtcaccc tgaaagaagg
    70261 gatcgaaaag cagttgctga acgaattccc ggagctgaaa ggtgtacgcg atctcaccga
    70321 acaccaacgc ggcgagcact cttactacta agttctctcc tcttttgtcg attgcccgat
    70381 gtgacgctaa agcgtcttat cgggcctgta cgttgcacga gagtctgaca ttagcgcaga
    70441 attacctcag caaatccaat ctcttcgttt ccccacgata atatgaccaa cctctcataa
    70501 tttaaattta ccccgctctg gtgattctca agcacgtaat gttacccgta tcattcccat
    70561 gggcaccaaa cacactcata acatctgact acactaactc gttttagggg tcatcagccg
    70621 tggtgccgta acatctctgt tcccagttgg gacaaccagg attttccgtc agaaacctga
    70681 cgttacccat aacaaattaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
    70741 tgtaatcttg ttattactcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
    70801 cctcgtggcg cttgctgttg gattaatgca agggatgccg ctggataaag ttattggctc
    70861 catcaaagcc ggtgtcggcg gaacgctcgg tagccttgcc ctgatcatgg gttttggtgc
    70921 gatgctgggc aaaatgctgg cagactgcgg tggtgcacaa cgtatcgcta ccacgctgat
    70981 tgccaaattt ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg
    71041 ttttgccctg ttctatgaag tgggtttcgt gctgatgctg ccgctggtgt ttaccatcgc
    71101 ggcttccgcg aatattccac tgctgtatgt tggtgtacca atggcggctg cactgtctgt
    71161 gacccacggc ttcctgccac cgcatccggg tccgactgcg attgccacca ttttcaatgc
    71221 cgatatgggt aaaactctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
    71281 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccgatcccgg aaggtctgta
    71341 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
    71401 tctggtgccg gtggttctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
    71461 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcta cgctgattgc
    71521 cgtgctgatc gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
    71581 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatta tcggtggtgg
    71641 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
    71701 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
    71761 cgcgctgggt tccgcaaccg tcgcggcaat cactgcgggt ggtatcgcgg caccgctgat
    71821 tgcaacgacg ggtgttagcc cggagctgat ggttattgcg gttggttccg gtagtgtgat
    71881 tttctctcac gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
    71941 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
    72001 aggctgtctg ctgctgaata tggtgatttg ataatactga tggccggatg cggcgtgaac
    72061 gccttatccg gcctacgatt cggcattaat tagtaggccg gataagacgc gtcaagcgtc
    72121 gcatccggca tcaaccgcac tctacttctt cttcgctgcc gctctgcgcc gtctgtccaa
    72181 atccttcagc aatttgttca caccatcatc ggcaaacatc gactcaagcg ttgcggaaag
    72241 tttacgtcgc cagtttttat actggtaact ggtgccggga atatttaccg gatctgccat
    72301 atccagccag tcttccggct ggagccctaa cagggcgctg ttgctatcgg cgatgtagcg
    72361 ctgtaagcca cgattcagcg tcggtgtcat cgacatcaac gatgccttat gcccggcacg
    72421 ttttggcagg caaccatatt tatgcagagc atccagcagc ccttgcttcg ccagttcgcg
    72481 atcctgatac agaccgcgca gtaccacttc atccggatac agccccagcg ttttgcccag
    72541 cgttaaatcc ccactctccc agtagccgcg tagcgtaggc aggtcgtgtg tcgccgcaac
    72601 cgccatcgac tgctctgggt acgctttcgg tgcgcggaat gtcttctcgt ggtcgttttc
    72661 gaaatagagc actttgtagg agtacacacc gctgctgcgc agcttaccga caatctctac
    72721 cggtacggta ccgagatctt caccaatcac catacagcga tgacgtttac tttcgagtgc
    72781 cagaatcgag agcagatcat ccaccggata gtgaacatac gcgccctgat ctgccgtctc
    72841 gccatacggt atccaccaca aacgcagcat cgacatcacg tgatcaattc gtaatgcgcc
    72901 gcagttttgc atattggcac gcaacagctc gataaacggt tcataggcac gcgcggtgat
    72961 gatatgcggg tccattggcg gcaatcccca gttctgcccc aacgggccga ggatatccgg
    73021 cggcgcgcca accgacgctt tcaggcaata cagttcacgg tcgcaccagg tttctgcccc
    73081 accttctgct acaccaacgg ccagatcacg atacaagcca atcggcattt catagccctg
    73141 gcttatctcc cagcaggcgg caaactggct gtaagccagc cactgcaacc agagataaaa
    73201 atcgacgtca tcacgatgtt cttcgcagaa ctggcgcact tctggtgaat ccacgttctg
    73261 atacatctct gaccatgctg gccagcccca gcgcatttcg tcctctttca cttgctgggc
    73321 atgtagcgca tcaaaggctg cctgccagaa caggctgtcg ccctgctctg caacaaactg
    73381 gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa
    73441 tgctgtcatt tttagggcgg taaccgtgga gtaatcaacc cagtcggcat cccgcgcctg
    73501 ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc tgagcctctt cgctaagatg
    73561 gaaatcttca actgcgttaa cgtcgatata aatcacattc agccaacggc gagaagacgg
    73621 gctgtacggg ctggcacttt ccggatttgc cagatagagc gcatgaatcg ggttcagacc
    73681 aataaacgac ccgccacgtt ttgccacatc caccagcatc gctttgagat cgccaaaatc
    73741 cccaataccc cagttttttt ccgatcgcag cgtataaagc tgaacgcagg caccccacag
    73801 cttttgttta ttcagcaacg cctgcggttc gtaacagcgt ttcggggcga caatcacccg
    73861 gcaatgcgcg cgctggtcgt cctgggtaag cgtcagcgtg tgataacctt ccggcagctt
    73921 cgtcggtaga ttgaacgctt tgccccccgt cacatggcct ttgtactgct ttccttcttc
    73981 ggtggtcagc agccagctat attcgccgct gccctccacc accatcggca tttttttgcc
    74041 gctggtataa accatgacat tcgggactgg cgttaccgcc actttcgtgg cggtacgttg
    74101 atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg
    74161 ggcattgatg taattggggc tgatccccgc cgccagcgcg gcattatcca gacgtttgct
    74221 ttccatcgag cttccttagc gttttgcctg ccagatacga gcctgataat cgcgaataga
    74281 gcgatccgag ctaaacatac cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc
    74341 ctcctggtcg cggtacagca catccacctg cttttgtgcc tctacataag cggcgaaatc
    74401 tgccatcacc agatacggat cgccgccctg tttgccgata ctgtgcagca tctggtcgaa
    74461 ggcatgctta tcgccgtcgc tgtatttacc gctttccagc tctttcaata ctgcgtccag
    74521 caccttatct ttcttccgcc atttcaccgg gtcgtagcct ttggccagaa ttgccttcac
    74581 ttgttccacg gtatgaccaa aaataaagat attttcttca ccgactttct cagcgatttc
    74641 aacgttcgca ccatccagcg taccgacagt aagcgcacca ttgagcgcca gtttcatatt
    74701 gccggtaccg gaagcttctt tacctgcagt cgaaatttgt tcggagatat ccgccgccgg
    74761 gatcagtttt tccgccgccg aaacacaata atccggcaag aacaccacct tcaacttgtc
    74821 gccaaccagc ggatcgttgt tgatcacgtc agccactttg ttgatcgcaa agataatatt
    74881 tttcgccagg tagtagcccg gtgctgcttt cgcgccgaag aggaagacgc gcggtacgcg
    74941 gtcagactgc gggttttcac gaatttcttt gtacaacgcc agaatatgca gcagattcag
    75001 atgctggcgt ttgtactcat gcagacgttt gatctgaata tcgaaaatcg cctgtggatt
    75061 gatctcaata ccggtacgaa ctttcacaaa ctctgccaga cggactttat tcgcctgctt
    75121 gatctcgcga tattgctgac ggaatttcgc atcatcagcg aatttttcca gattgatcag
    75181 ttgatcgaga tcgttagccc actctttttt cagtgattta tccaacagag ccgccagtgc
    75241 cgggttgcac tgtttgatcc agcgacgtgg ggtaataccg ttagtgacat tatggaattt
    75301 gttcggccat agctggtgat attccgggaa cagatctttc accaccagat ccgagtgcag
    75361 cgccgcaaca ccgttcacag cgaaaccgcc aaccacacac aggttcgcca tatgcacttg
    75421 tttgtcgtgc accaccgcca gtttggccca cactttttca tcgcccggcc aggttttttc
    75481 caccagcgtt ttaaagcgtg tattaatttc gttaataatc tgcatgtggc gcggcagtaa
    75541 gcctttcacc agtttcacat cccagcgttc cagcgcttct ggcatcaggg tatggttggt
    75601 gtaagcaaaa gttttactgg tgatggccca ggcgtcatcc cagctcatct ggtgctcatc
    75661 gatcagcacg cgcagcagtt ctggaatcgc gatagttggg tgggtatcgt tcagctgaat
    75721 aacttcataa tccgccagtt cgtgcagttt acgccccgcc agatgatggc gacgcaaaat
    75781 atccgctacc gaacaggcac actggaagta ttgctgcatc aggcgcagct ttttaccggc
    75841 agtatggtta tcgtttggat agagaacttt ggtcagtttt tccgcgttga tgccctgctg
    75901 ttcggcacgc aggaaatcac catcgttaaa tttagtcaga tcaaacggat gcgcatgcgt
    75961 cgcctgccac agacgcagcg gctgcgccac gccattacga tagccgacaa cggggagatc
    76021 ccacgcttga ccggtaatgg taaactccgg ctcccagcgt ccgtctttcg tcactttacc
    76081 gccaatccct acctgcacat ccagtgcttc gttgtggcgg aaccacgggt aattaccgcg
    76141 atgccagtca tccggcgctt caacctgttt gccatcgaca aaagactggc ggaacaaacc
    76201 atattgatag ttcagaccgt aacccgtcgc agactgaccg acagttgcca ttgagtcgag
    76261 gaagcacgcc gccagacgtc ccagaccacc gttccccagc gccgggtcga tctcttcttc
    76321 caacaggtca gtcaggttga tgtcataagc cttcaacgaa tcctgtacat cctgatacca
    76381 gccgagattc aacaggttgt tgcccgtcag gcgaccaatt aaaaactcca ttgagatgta
    76441 gttaacatgt cgctgattcg ccactggctt ggcgaatggc tgagcacgca gcatttcggc
    76501 cagtgcttcg ctcactgcca gccaccactg gcgaggagtc atttcagccg cagaatttaa
    76561 gccataacgc tgccactgac gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg
    76621 ttgtgacata ggagttccac ttttcttaga ttttcaacac aacgttatcg ctagtttgcc
    76681 aggctcgatg ttgaccttcc tcatcctgcg ggggattagg cagggaggag ttgcggggat
    76741 gagcaaggaa atgtgatctc gaccacttaa agctagtgca aaccacagga ttagcagcaa
    76801 atcaatgcca taccgcgcag aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt
    76861 ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga gtgaaacagt ttcacaagta
    76921 aaatatcaaa tgtgctccat ctcattctta atagatttat taagatcatc tttttagatg
    76981 gcactttcat cagaaatgaa gaagaaaccc ttacttaaat gaaactgatg aacataagga
    77041 aaataccgta caacccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
    77101 aacatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
    77161 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
    77221 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaat taagtcgtcc
    77281 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
    77341 caatttccgg ctggcgctga tcacaagtcc tgcgggctac ggaaagacca cgctcatttc
    77401 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca
    77461 gcaagagcgt ttcgccagct atctcattgc cgccgtgcaa caggcaacca acggtcactg
    77521 cgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
    77581 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
    77641 tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc
    77701 agaaaatctc acccttgtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
    77761 gcgtgttcgt gaccagctgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
    77821 agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagctgcag aaagcagtcg
    77881 gatttgtgat gatgtttccg gttgggcgac ggcactgcag ctaatcgccc tctccgcccg
    77941 gcagaatact cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
    78001 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
    78061 tctgttgaaa agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg
    78121 cgaagaaaac gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg
    78181 gatggatgat accggcgagt ggttttgcta tcacccactg tttggtaact tcctgcgcca
    78241 gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag
    78301 ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc
    78361 gctgatgctg cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact
    78421 gtcgctgctg gaagagtcac ttaaggccct gccgtgggac agtttgctgg aaaatccgca
    78481 gttggtgtta ttgcaggcgt ggctgatgca aagccagcat cgctacggcg aagttaacac
    78541 cctgctggcc cgcgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga
    78601 atttaacgct ctgcgcgccc aggtggcgat taacgatggt aacccggatg aagcggaacg
    78661 gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc
    78721 aacctcggtg ctgggtgaag tgttgcactg caaaggcgaa ttgacccgct cactggcgtt
    78781 aatgcagcaa accgaacaga tggcacgcca gcacgatgtc tggcactacg ctttgtggag
    78841 tttaatccag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac
    78901 gcaggaaaaa gcgttccagc tgatcaacga gcagcatctg gaacagctgc caatgcatga
    78961 gtttctggta cgcatccgtg cgcagctgtt atgggcctgg gcacggctgg atgaagctga
    79021 agcgtcggcg cgcagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca
    79081 gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag
    79141 ccagctgaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc
    79201 taacgccaac aaagtccggg tgatttactg gcaaatgacc agcgataaag ccgccgctgc
    79261 caactggttg cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca
    79321 atggcgcaac attgcccgtg cacaaatctt gctgggcgag tttgaatctg cggaaattgt
    79381 cctcgaagaa ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa
    79441 cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgtgt
    79501 gttgctggac gcattaaaac tggcgaatcg caccggattt atcagtcatt ttgtcatcga
    79561 aggcgaagcg atggcgcaac aactgcgcca gcttattcaa ctcaatacgc tgccggagct
    79621 ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt
    79681 cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagagct
    79741 gatccgcacc agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg
    79801 ttacagcaat gagcaaattg caggcgagct ggaagtagcg gcaaccacca tcaaaacgca
    79861 tatccgcaat ctgtaccaaa aactcggtgt cgcccaccgt caggccgcgg tacaacacgc
    79921 gcagaaactg ctgaagatga tgggatatgg agtgtgagtt tagccggata atacgtcgga
    79981 tccggcttac atctttatat cattcaatgc tcacccgcgt tacgccatcc gcctccacca
    80041 gactaaaccg cactggcaag aaacgctcca ccaccgcaat attggtcagc agatggcatg
    80101 agggatgggc gaccgtaaat tctcccgcgc ccgctaacgc cattggcagt accagctggt
    80161 cagcgagata ctcccctacc gccgcagggc tcgccaggta acgtttcacc tcttgcacta
    80221 actgcgcagc caccacttcg gcactgacgc gcttttcacc gacgacaaaa aagagttcgg
    80281 tgatattttc actttcgact tcaagcgaga cggtattacc cggcccctgg tcgcgcggca
    80341 ggttatgaat attctgttca tgcaaggaaa aactacccac cagtgtagcg atttcacgct
    80401 cagcgacatg gcgcggcacg ccagctaata gcacctctcc acgcatctgc acaatgttcc
    80461 cgcgctcgcc aagttgcaag ctattaaacg atgccaccgg tgagacttcc gttgctacca
    80521 cgccgcctcc ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaactccta
    80581 ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggcggg gccgacgggt
    80641 tatcggtgcc gccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca
    80701 gcaccgtttg cagcaccagc gtacaacttc cggcgctacc gatagcaaag cggtaatcgc
    80761 cgccgcgcac ggtgccaggt cggaagacca gacgctgcga ccccagctca gcgccttcca
    80821 ccgttgccct acaaatttcc gcagccgctt ttaccgcggt cagatgttgg cgcaacagcc
    80881 ccggtttcgc ccgcccggca cgaatgccgg tgatggtaaa tggctggccg gttatcatcg
    80941 acaggctcag cgccgagcgc aggatctgcc cgccgccttc gccctgtgcg ccatccagcg
    81001 caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa
    81061 cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga
    81121 tttcgtcgat cacctcggca tctttacggc actccacatg cgcggtggca cggatttgat
    81181 cttccacact gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc
    81241 tgcacgaaca gaacgactct tcatttccca gcccacggac gataaagctt ttcgccccca
    81301 tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcacct ttacgcgtca
    81361 catagatctc ttcgccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct
    81421 cttccatcgc cagcgtttgt ggctgtttta ccgttttctg cgtaacgctc tgcaacgccg
    81481 ttaccacgtt ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaagcca
    81541 cggctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc aggtcacgcg
    81601 atggcaacgt ttcaagcgtt tcctgcatct ctttttgcgc cagatcgata aaataagttc
    81661 cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact
    81721 catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg tgtttatagt
    81781 tgttagtgtt aaggaaacgg ggatattttt gcgttaacca ctgataaccg gcttccagct
    81841 cagcccattt cgcatcgacg ttaacaggcg gattttccca tgcgccttta tcacgtttac
    81901 aacgcccggt agtacgtccg tgcggtacgg ccgtttcaat cgcctgacgc agctctgcca
    81961 ggttttcagg caggtccgcc gccgttaacg cggtacgcag tgcgttcatt ccacagccaa
    82021 tatccacgcc caccgccgcc ggaataatcg cccctttggt cggaatcacg ctaccaatgg
    82081 tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca
    82141 tcttcgcggt attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc
    82201 acatttttac cggagcattt tcagtggtca gtaattcgta attcattttg tttttctctt
    82261 ttcgttgttt gctgtcctga taa
//
LOCUS       DAZFRE010000008        80788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFRE010000008 DAZFRE010000000
VERSION     DAZFRE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.008"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            309..1496
                     /gene="ygeW"
                     /locus_tag="ACHKUI_000648"
     CDS             309..1496
                     /gene="ygeW"
                     /locus_tag="ACHKUI_000648"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6596911.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            1554..2750
                     /gene="dpaL"
                     /locus_tag="ACHKUI_000649"
     CDS             1554..2750
                     /gene="dpaL"
                     /locus_tag="ACHKUI_000649"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6596912.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            2808..4019
                     /gene="ygeY"
                     /locus_tag="ACHKUI_000650"
     CDS             2808..4019
                     /gene="ygeY"
                     /locus_tag="ACHKUI_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6596913.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            4072..5457
                     /gene="hyuA"
                     /locus_tag="ACHKUI_000651"
     CDS             4072..5457
                     /gene="hyuA"
                     /locus_tag="ACHKUI_000651"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6596914.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            5505..6437
                     /gene="arcC"
                     /locus_tag="ACHKUI_000652"
     CDS             5505..6437
                     /gene="arcC"
                     /locus_tag="ACHKUI_000652"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6596915.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINHAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6478..8103)
                     /gene="yqeB"
                     /locus_tag="ACHKUI_000653"
     CDS             complement(6478..8103)
                     /gene="yqeB"
                     /locus_tag="ACHKUI_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6596916.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(8151..8921)
                     /gene="yqeC"
                     /locus_tag="ACHKUI_000654"
     CDS             complement(8151..8921)
                     /gene="yqeC"
                     /locus_tag="ACHKUI_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6596917.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEVIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            9025..9603
                     /gene="mocA"
                     /locus_tag="ACHKUI_000655"
     CDS             9025..9603
                     /gene="mocA"
                     /locus_tag="ACHKUI_000655"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6596918.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            9925..13023
                     /gene="ygfK"
                     /locus_tag="ACHKUI_000656"
     CDS             9925..13023
                     /gene="ygfK"
                     /locus_tag="ACHKUI_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6596919.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWEEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTTLKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLSDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSITVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            13026..14354
                     /gene="ssnA"
                     /locus_tag="ACHKUI_000657"
     CDS             13026..14354
                     /gene="ssnA"
                     /locus_tag="ACHKUI_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6596920.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGVSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            14405..15184
                     /gene="ygfM"
                     /locus_tag="ACHKUI_000658"
     CDS             14405..15184
                     /gene="ygfM"
                     /locus_tag="ACHKUI_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6596921.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQNLELDWIDWDNGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            15181..18051
                     /gene="xdhD"
                     /locus_tag="ACHKUI_000659"
     CDS             15181..18051
                     /gene="xdhD"
                     /locus_tag="ACHKUI_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6596922.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            18216..19616
                     /gene="xanQ"
                     /locus_tag="ACHKUI_000660"
     CDS             18216..19616
                     /gene="xanQ"
                     /locus_tag="ACHKUI_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6596923.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            19634..20950
                     /gene="guaD"
                     /locus_tag="ACHKUI_000661"
     CDS             19634..20950
                     /gene="guaD"
                     /locus_tag="ACHKUI_000661"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6596924.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVYNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            20986..22353
                     /gene="ghxQ"
                     /locus_tag="ACHKUI_000662"
                     /gene_synonym="ygfQ"
     CDS             20986..22353
                     /gene="ghxQ"
                     /locus_tag="ACHKUI_000662"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6596925.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22389..22877)
                     /gene="ygfS"
                     /locus_tag="ACHKUI_000663"
     CDS             complement(22389..22877)
                     /gene="ygfS"
                     /locus_tag="ACHKUI_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6596926.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRIRQQRIAASGAN
                     PL"
     gene            complement(22877..24796)
                     /gene="ygfT"
                     /locus_tag="ACHKUI_000664"
     CDS             complement(22877..24796)
                     /gene="ygfT"
                     /locus_tag="ACHKUI_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6596927.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            25232..26680
                     /gene="uacT"
                     /locus_tag="ACHKUI_000665"
     CDS             25232..26680
                     /gene="uacT"
                     /locus_tag="ACHKUI_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6596928.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSLGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            26682..26807
                     /gene="yqfG"
                     /locus_tag="ACHKUI_000666"
     CDS             26682..26807
                     /gene="yqfG"
                     /locus_tag="ACHKUI_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6596929.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVVMAIESRFKYMMLLF"
     gene            complement(26804..26875)
                     /locus_tag="ACHKUI_000667"
     CDS             complement(26804..26875)
                     /locus_tag="ACHKUI_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596930.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            26930..27478
                     /gene="idi"
                     /locus_tag="ACHKUI_000668"
     CDS             26930..27478
                     /gene="idi"
                     /locus_tag="ACHKUI_000668"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6596931.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(27521..29038)
                     /gene="lysS"
                     /locus_tag="ACHKUI_000669"
     CDS             complement(27521..29038)
                     /gene="lysS"
                     /locus_tag="ACHKUI_000669"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6596932.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELHLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(29048..30146)
                     /gene="prfB"
                     /locus_tag="ACHKUI_000670"
     CDS             complement(join(29048..30070,30072..30146))
                     /gene="prfB"
                     /locus_tag="ACHKUI_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6596933.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(30237..31970)
                     /gene="recJ"
                     /locus_tag="ACHKUI_000671"
     CDS             complement(30237..31970)
                     /gene="recJ"
                     /locus_tag="ACHKUI_000671"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6596934.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSAAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(31976..32686)
                     /gene="dsbC"
                     /locus_tag="ACHKUI_000672"
     CDS             complement(31976..32686)
                     /gene="dsbC"
                     /locus_tag="ACHKUI_000672"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6596935.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(32711..33607)
                     /gene="xerD"
                     /locus_tag="ACHKUI_000673"
     CDS             complement(32711..33607)
                     /gene="xerD"
                     /locus_tag="ACHKUI_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6596936.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            33719..34240
                     /gene="fldB"
                     /locus_tag="ACHKUI_000674"
     CDS             33719..34240
                     /gene="fldB"
                     /locus_tag="ACHKUI_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6596937.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(34280..34687)
                     /gene="ygfX"
                     /locus_tag="ACHKUI_000675"
     CDS             complement(34280..34687)
                     /gene="ygfX"
                     /locus_tag="ACHKUI_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6596938.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(34668..34934)
                     /gene="sdhE"
                     /locus_tag="ACHKUI_000676"
     CDS             complement(34668..34934)
                     /gene="sdhE"
                     /locus_tag="ACHKUI_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6596939.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            35177..36157
                     /gene="ygfZ"
                     /locus_tag="ACHKUI_000677"
     CDS             35177..36157
                     /gene="ygfZ"
                     /locus_tag="ACHKUI_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6596940.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            complement(36234..36893)
                     /gene="yqfA"
                     /locus_tag="ACHKUI_000678"
     CDS             complement(36234..36893)
                     /gene="yqfA"
                     /locus_tag="ACHKUI_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6596941.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37057..37368)
                     /gene="yqfB"
                     /locus_tag="ACHKUI_000679"
     CDS             complement(37057..37368)
                     /gene="yqfB"
                     /locus_tag="ACHKUI_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6596942.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAKQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            37413..38846
                     /gene="bglA"
                     /locus_tag="ACHKUI_000680"
     CDS             37413..38846
                     /gene="bglA"
                     /locus_tag="ACHKUI_000680"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6596943.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            complement(39012..41885)
                     /gene="gcvP"
                     /locus_tag="ACHKUI_000681"
     CDS             complement(39012..41885)
                     /gene="gcvP"
                     /locus_tag="ACHKUI_000681"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6596944.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVVDKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(42003..42392)
                     /gene="gcvH"
                     /locus_tag="ACHKUI_000682"
     CDS             complement(42003..42392)
                     /gene="gcvH"
                     /locus_tag="ACHKUI_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6596945.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(42416..43510)
                     /gene="gcvT"
                     /locus_tag="ACHKUI_000683"
     CDS             complement(42416..43510)
                     /gene="gcvT"
                     /locus_tag="ACHKUI_000683"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6596946.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            43801..43884
                     /locus_tag="ACHKUI_000684"
     CDS             43801..43884
                     /locus_tag="ACHKUI_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596947.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            complement(43958..45160)
                     /gene="ubiI"
                     /locus_tag="ACHKUI_000685"
                     /gene_synonym="visC"
     CDS             complement(43958..45160)
                     /gene="ubiI"
                     /locus_tag="ACHKUI_000685"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6596948.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQWMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFSLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(45183..46361)
                     /gene="ubiH"
                     /locus_tag="ACHKUI_000686"
                     /gene_synonym="visB"
     CDS             complement(45183..46361)
                     /gene="ubiH"
                     /locus_tag="ACHKUI_000686"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6596949.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(46358..47683)
                     /gene="pepP"
                     /locus_tag="ACHKUI_000687"
     CDS             complement(46358..47683)
                     /gene="pepP"
                     /locus_tag="ACHKUI_000687"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6596950.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEHAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(47709..48287)
                     /gene="ygfB"
                     /locus_tag="ACHKUI_000688"
     CDS             complement(47709..48287)
                     /gene="ygfB"
                     /locus_tag="ACHKUI_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6596951.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            48455..48784
                     /gene="zapA"
                     /locus_tag="ACHKUI_000689"
     CDS             48455..48784
                     /gene="zapA"
                     /locus_tag="ACHKUI_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6596952.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            48826..49009
                     /gene="ssrS"
                     /locus_tag="ACHKUI_000690"
     ncRNA           48826..49009
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKUI_000690"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            49030..49632
                     /gene="fau"
                     /locus_tag="ACHKUI_000691"
     CDS             49030..49632
                     /gene="fau"
                     /locus_tag="ACHKUI_000691"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6596953.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVVTPSKVWEW"
     gene            complement(49734..49793)
                     /gene="ibsC"
                     /locus_tag="ACHKUI_000692"
     CDS             complement(49734..49793)
                     /gene="ibsC"
                     /locus_tag="ACHKUI_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6596954.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(50022..51254)
                     /gene="serA"
                     /locus_tag="ACHKUI_000693"
     CDS             complement(50022..51254)
                     /gene="serA"
                     /locus_tag="ACHKUI_000693"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6596955.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(51510..52169)
                     /gene="rpiA"
                     /locus_tag="ACHKUI_000694"
     CDS             complement(51510..52169)
                     /gene="rpiA"
                     /locus_tag="ACHKUI_000694"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6596956.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(52225..52455)
                     /locus_tag="ACHKUI_000695"
     CDS             complement(52225..52455)
                     /locus_tag="ACHKUI_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6596957.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            52596..53489
                     /gene="argP"
                     /locus_tag="ACHKUI_000696"
     CDS             52596..53489
                     /gene="argP"
                     /locus_tag="ACHKUI_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6596958.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            53692..54571
                     /locus_tag="ACHKUI_000697"
                     /pseudo
     CDS             53692..54571
                     /locus_tag="ACHKUI_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
     gene            54596..54748
                     /locus_tag="ACHKUI_000698"
                     /pseudo
     CDS             54596..54748
                     /locus_tag="ACHKUI_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
     gene            complement(54745..55635)
                     /gene="ygfI"
                     /locus_tag="ACHKUI_000699"
     CDS             complement(54745..55635)
                     /gene="ygfI"
                     /locus_tag="ACHKUI_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6596959.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASRFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFNISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPT
                     IIYRPYSFMAKISAVEREHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRN
                     HRYTATMLQHIAEDRDGTNH"
     gene            complement(55793..56533)
                     /gene="yggE"
                     /locus_tag="ACHKUI_000700"
     CDS             complement(55793..56533)
                     /gene="yggE"
                     /locus_tag="ACHKUI_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6596960.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(56626..57261)
                     /gene="argO"
                     /locus_tag="ACHKUI_000701"
     CDS             complement(56626..57261)
                     /gene="argO"
                     /locus_tag="ACHKUI_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO6596961.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(57400..58260)
                     /gene="mscS"
                     /locus_tag="ACHKUI_000702"
     CDS             complement(57400..58260)
                     /gene="mscS"
                     /locus_tag="ACHKUI_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6596962.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(58451..59530)
                     /gene="fbaA"
                     /locus_tag="ACHKUI_000703"
     CDS             complement(58451..59530)
                     /gene="fbaA"
                     /locus_tag="ACHKUI_000703"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6596963.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(59745..60908)
                     /gene="pgk"
                     /locus_tag="ACHKUI_000704"
     CDS             complement(59745..60908)
                     /gene="pgk"
                     /locus_tag="ACHKUI_000704"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6596964.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(60958..61977)
                     /gene="epd"
                     /locus_tag="ACHKUI_000705"
     CDS             complement(60958..61977)
                     /gene="epd"
                     /locus_tag="ACHKUI_000705"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6596965.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            62349..62777
                     /locus_tag="ACHKUI_000706"
     CDS             62349..62777
                     /locus_tag="ACHKUI_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6596966.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            62804..63382
                     /locus_tag="ACHKUI_000707"
     CDS             62804..63382
                     /locus_tag="ACHKUI_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6596967.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            63383..64090
                     /locus_tag="ACHKUI_000708"
     CDS             63383..64090
                     /locus_tag="ACHKUI_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6596968.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     TMILLMLAEFGAWIWLR"
     gene            64078..64755
                     /locus_tag="ACHKUI_000709"
     CDS             64078..64755
                     /locus_tag="ACHKUI_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6596969.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            64749..65426
                     /locus_tag="ACHKUI_000710"
     CDS             64749..65426
                     /locus_tag="ACHKUI_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6596970.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQDAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVASLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(65398..66111)
                     /locus_tag="ACHKUI_000711"
     CDS             complement(65398..66111)
                     /locus_tag="ACHKUI_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6596971.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(66108..66617)
                     /gene="fumE"
                     /locus_tag="ACHKUI_000712"
     CDS             complement(66108..66617)
                     /gene="fumE"
                     /locus_tag="ACHKUI_000712"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6596972.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQN
                     EMPGFADDMAWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(66639..66954)
                     /locus_tag="ACHKUI_000713"
                     /pseudo
     CDS             complement(66639..66954)
                     /locus_tag="ACHKUI_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(66951..68228)
                     /gene="yggP"
                     /locus_tag="ACHKUI_000714"
     CDS             complement(66951..68228)
                     /gene="yggP"
                     /locus_tag="ACHKUI_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6596973.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLTQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVYIGEAAQ"
     gene            complement(68243..69631)
                     /gene="cmtA"
                     /locus_tag="ACHKUI_000715"
     CDS             complement(68243..69631)
                     /gene="cmtA"
                     /locus_tag="ACHKUI_000715"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6596974.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(69659..70102)
                     /gene="cmtB"
                     /locus_tag="ACHKUI_000716"
     CDS             complement(69659..70102)
                     /gene="cmtB"
                     /locus_tag="ACHKUI_000716"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6596975.1"
                     /translation="MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQ
                     AIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            70478..71386
                     /locus_tag="ACHKUI_000717"
     CDS             70478..71386
                     /locus_tag="ACHKUI_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO6596976.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(71606..73597)
                     /gene="tkt"
                     /locus_tag="ACHKUI_000718"
     CDS             complement(71606..73597)
                     /gene="tkt"
                     /locus_tag="ACHKUI_000718"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6596977.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            73875..74633
                     /gene="loiP"
                     /locus_tag="ACHKUI_000719"
                     /gene_synonym="yggG"
     CDS             73875..74633
                     /gene="loiP"
                     /locus_tag="ACHKUI_000719"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6596978.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIHDRMSADGVK"
     gene            complement(74724..75644)
                     /gene="speB"
                     /locus_tag="ACHKUI_000720"
     CDS             complement(74724..75644)
                     /gene="speB"
                     /locus_tag="ACHKUI_000720"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6596979.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(75780..76511)
                     /locus_tag="ACHKUI_000721"
     CDS             complement(75780..76511)
                     /locus_tag="ACHKUI_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6596980.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(76657..78633)
                     /gene="speA"
                     /locus_tag="ACHKUI_000722"
     CDS             complement(76657..78633)
                     /gene="speA"
                     /locus_tag="ACHKUI_000722"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6596981.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(78642..78773)
                     /gene="yqgB"
                     /locus_tag="ACHKUI_000723"
     CDS             complement(78642..78773)
                     /gene="yqgB"
                     /locus_tag="ACHKUI_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6596982.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(78867..79010)
                     /gene="yqgG"
                     /locus_tag="ACHKUI_000724"
     CDS             complement(78867..79010)
                     /gene="yqgG"
                     /locus_tag="ACHKUI_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6596983.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(79121..79372)
                     /gene="yqgD"
                     /locus_tag="ACHKUI_000725"
     CDS             complement(79121..79372)
                     /gene="yqgD"
                     /locus_tag="ACHKUI_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6596984.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            79428..80582
                     /gene="metK"
                     /locus_tag="ACHKUI_000726"
     CDS             79428..80582
                     /gene="metK"
                     /locus_tag="ACHKUI_000726"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6596985.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        19838 a        21362 c        20080 g        19508 t
ORIGIN      
        1 tacagcggca agtttatcaa actgaagaga gatagcctgc ctctttatct tatttctgat
       61 acttagcagc aaataaataa cgtgataaaa aaagccaaac gttttcgtat tttaaaaaca
      121 accggaaact ggcatcaata tgtgatcaac cccacacatt atccgtcaaa ttagcctttc
      181 gcagccgcgt ggataattct ggcacactta ttgttagtcc caggtatagc tgtgaaaaca
      241 ccaatcactt tggcacgtca cagtgaaata aaccactttg cctgtcattc cactatcggg
      301 actttatgat gaaaactgtt aatgagctga ttaaggatat caattcgctg acctctcacc
      361 tgcatgaaaa agattttttg ttaacgtggg aacagacgcc agacgaactg aaacaagtac
      421 tggacgttgc cgccgcgtta aaagcactgc gtgctgaaaa catctcaacc aaagtcttta
      481 atagtggatt aggtatttcc gtattccgcg acaattccac ccgtacccgc ttctcttatg
      541 cttccgcgct taacctgctc ggccttgcac aacaagatct tgacgaaggt aaatcacaaa
      601 tcgctcacgg cgaaaccgtg cgtgaaaccg ccaatatgat ctccttctgc gctgacgcta
      661 tcggtattcg cgacgatatg tatctgggcg caggcaacgc ctatatgcgt gaagttggcg
      721 ctgcacttga tgacggctac aagcagggtg tgctgccgca gcgtccggct ttagtgaacc
      781 tgcaatgcga tattgaccac ccgactcagt ctatggctga cctggcatgg ttgcgtgaac
      841 actttggttc gctggaaaac ctgaaaggca aaaaaatcgc catgacctgg gcctactccc
      901 caagctatgg caaaccgctc tctgtaccac aaggcatcat aggtctgatg acccgcttcg
      961 gtatggatgt caccctggcc catccggaag gctacgacct gatcccggat gtcgttgaag
     1021 ttgctaaaaa caatgctaaa gcctccggtg gtagcttccg tcaggtcacc agcatggaag
     1081 aagccttcaa agacgcagac atcgtttatc cgaagtcatg ggcaccttac aaagtgatgg
     1141 aagagcgtac tgaattgctg cgtgcgaacg atcacgaagg cttaaaagca ctggaaaaac
     1201 agtgtctggc acagaacgcg caacacaaag actggcattg tactgaagag atgatggaac
     1261 tgacccgtga tggcgaagcc ctgtacatgc actgcctgcc agctgatatc agcggcgtat
     1321 cctgcaaaga aggtgaagtg actgaaggcg tattcgaaaa ataccgtatc gctacctaca
     1381 aagaagccag ctggaagcct tatatcatcg ccgcgatgat cctgtcccgt aaatacgcca
     1441 aaccaggtgc actgctcgag caactgctga aagaagcgca agaacgcgtg aaataacccc
     1501 tcgggccgat cagctggtcg gccctctatt tccagaggcc aaaaggatag gatatgtccg
     1561 ttttctcatt gaagattgat atcgccgata acaaattttt caacggcgaa acatcaccgc
     1621 ttttttcgca aagccaggca aaactggcac gccagttcca ccagaaaatt gctggttatc
     1681 acccaacacc gctttgtgcg ctggacgatc tcgcaaacct gtttggtgta aagaaaattc
     1741 tcgtcaaaga cgaatcaaaa cgattcggtc tgaacgcctt caaaatgctc ggcggtgcgt
     1801 acgccatcgc tcaattattg tgtgaaaaat atcatcttga tatcgaaacg ctgtcatttg
     1861 agcacctgaa aaatgccatc ggcgaaaaaa tgactttcgc gactaccacc gacggcaacc
     1921 acgggcgcgg tgtggcatgg gcagcacagc aactcggaca gaatgcggtg atttacatgc
     1981 cgaaaggttc tgctcaggaa cgcgttgacg ccattctaaa cctcggtgcc gagtgcatcg
     2041 tcacggatat gaactatgac gataccgttc gcctgaccat gcaacacgcg cagcagcacg
     2101 gctgggaagt ggtacaagac acggcatggg aaggttacac caaaatccca acctggatca
     2161 tgcaaggcta cgcaacactg gcggatgaag ccgtcgagca aatgcgtgaa atgggcgtca
     2221 ccccgacaca cgttctgctg caagccggtg tcggtgcaat ggccggtggt gtgctgggtt
     2281 atctggtcga cgtctatagc ccgcaaaatc tgcacagcat tattgttgaa cctgacaaag
     2341 ctgactgtat ttatcgctcc ggcgtcaaag gcgacatcgt caacgttggc ggtgatatgg
     2401 ccaccatcat ggcgggcctg gcctgcggcg aacctaaccc actgggctgg gaaatcctac
     2461 gtaactgcgc cacccaattc atctcctgcc aggacagcgt tgccgcatta ggtatgcgcg
     2521 tgctgggtaa tccgtatggc aacgacccgc gcatcatctc cggtgaatcc ggcgctgtcg
     2581 gtttgggcgt tctcgcagcg gttcattatc acccgcaacg tcaaagcctg atggaaaaac
     2641 tggcgctgaa caaagatgcc gtggtgctgg ttatcagcac tgaaggcgac accgacgtga
     2701 agcactaccg cgaagttgtc tgggaaggca aacacgctgt agcaccttaa tcaccctatt
     2761 ggaactgctc cctgcaaaac ggggagtaaa aaatctggag aaaaataatg gctaagaata
     2821 ttccattcaa actgattctt gaaaaagcaa aagattacca ggcagacatg actcgcttcc
     2881 tacgcgatat ggttgctatt cccagtgaaa gctgcgacga gaaacgcgta gtacatcgta
     2941 ttaaagaaga gatggaaaaa gtcggcttcg ataaagttga aatcgaccca atgggcaacg
     3001 ttctcggtta tatcggccac ggcccgcgtc tggtggcaat ggacgctcat atcgatacag
     3061 tcggcattgg caacatcaaa aactgggact tcgatccgta tgaaggcatg gaaaccgacg
     3121 agctgatcgg tggtcgtggt acttccgacc aggaaggcgg catggcgtct atggtatatg
     3181 ccggaaaaat cattaaagat ctcggtctgg aagatgaata taccctgctg gtcaccggta
     3241 ctgtgcagga agaagactgc gacggtctgt gctggcaata cattattgaa caatccggca
     3301 ttcgcccgga atttgtggtc agtaccgaac caaccgactg ccaggtatac cgtggtcaac
     3361 gcggtcgtat ggaaattcgc attgatgttc agggtgttag ctgccacggt tcggcaccag
     3421 aacgtggtga taacgccatt ttcaaaatgg gtccaattct tggcgaatta caggaactgt
     3481 ctcaacgtct gggctatgac gaattcctcg gcaaaggtac gctgactgtt tctgaaatct
     3541 tcttcacctc cccaagtcgt tgcgctgtag cagacagctg cgcagtttca atcgaccgtc
     3601 gtctgacctg gggcgaaacc tgggaaggcg cgctggacga gatccgcgct ctacctgcag
     3661 tacagaaagc taacgcggtt gtttctatgt acaactacga ccgcccgtcc tggactggcc
     3721 tggtttaccc aaccgaatgc tacttcccga cctggaaagt ggaagaagat cacttcaccg
     3781 ttaaagcact ggtgaatgct tatgaagggc tgtttggtaa agcgcctgtc gttgataagt
     3841 ggaccttctc gactaatggc gtatccatca tgggtcgcca cggcattccg gtcatcggtt
     3901 ttggccctgg taaagaaccg gaagcgcacg cgccaaacga aaaaacctgg aaatctcacc
     3961 tggtaacctg tgccgctatg tacgctgcaa tcccattaag ctggctggca accgaataat
     4021 tactcttcac ttattcccct ccggttcgcc ggagggtttt tggagtttgc tatgcgcgta
     4081 ttgataaaaa acggcattgt tgttaatgca gatggacagg cgaagcagga tttgctgatt
     4141 gaaagcggga ttgttcgcca gttgggtacc gatatttcgc cgcaactccc ttgcgaagaa
     4201 attgatgcct ctggctgtta cgttttccct ggcggcgtgg atgtccatac gcatttcaat
     4261 attgatgtcg gcatcgcgcg cagttgtgat gattttttta ccggtacccg cgctgctgcg
     4321 tgtggcggta caacaaccat tattgaccat atgggatttg gcccaaatgg ctgccggtta
     4381 cgccatcaac tggaagttta tcgcggttat gccgcccaca aagcagtcat cgactacagc
     4441 tttcacggtg tcattcaaca tattaatcac gctatcctcg acgaaattcc gatgatggtt
     4501 gaggaaggac tgagcagttt taaactctat ttaacctatc aatataaact caacgatgat
     4561 gaagtgttgc aggcattacg ccgcttgcat gagtccggcg cgctgaccac cgtacacccg
     4621 gaaaatgacg cagctatcgc cagcaagcgg gcggagttta tcgccgcagg gttaaccgcg
     4681 ccgcgttatc atgccttgag tcgacctctg gaatgcgaag cggaagccat cgcccgcatg
     4741 attaacctgg cacaaattgc cggtaacgcc ccgctctata tcgtgcacct gtctaacggc
     4801 ttaggtctgg attatctgcg tcttgcccgt gcgaatcacc agccagtctg ggttgaaacc
     4861 tgcccacaat atctcctgtt ggacgaacgc agttacgata cagaagatgg catgaagttc
     4921 attcttagcc caccgctgcg taacgtacgc gagcaggaca aactgtggtg tggcatcagc
     4981 gatggtgcga ttgacgtggt ggcaaccgat cactgcacct tctcgatggc tcaacgcctg
     5041 caaatttcta aaggcgattt cagccgctgc ccaaatggct tacccggtgt ggagaaccgc
     5101 atgcaactgc tgttctccag tggcgtgatg acgggacgta tctcaccgga gcgttttgtt
     5161 gagttaacca gtgcaatgcc cgccaggctg tttggcctgt ggccacaaaa aggattatta
     5221 gcgcccggtt ccgatggcga cgtggtgatt atagacccac gtcagagcca acaaattcag
     5281 catcgccatc tccacgacaa cgctgactac tcgccatggg aggggtttac ctgtcagggc
     5341 gcgattgtca gaaccttatc ccgtggtgaa acgattttct gcgacggcac ctttacaggc
     5401 aaagccgggc gaggtcgttt cctgcgacgc aaaccgtttg tccctcccgt gctctaacca
     5461 ctgccaggga tctatacaca atcaagaatg atgagtgagg aaaaatgagt aagaaaattg
     5521 ttctcgccct gggcgggaat gcgctgggcg atgacctggc tgggcaaatg aaagcggtaa
     5581 aaattacttc tcaggcaatt gttgatttaa ttgcccaggg acatgaggtc atcgtcactc
     5641 atggcaacgg gccacaggtg ggcatgatta accatgcctt tgaagctgca gcaaaaactg
     5701 aagcgcactc gccaatgctg ccgatgtctg tctgtgttgc cttaagccag ggttatattg
     5761 gttacgatct acaaaacgcc ttaagagaag aactgctttc tcgtggcatt aataaacctg
     5821 tagcaacgct ggttacccag gtcgaagtcg atgctaacga tccggcattc ctcaacccga
     5881 ccaagccgat cggttcgttc tttaccgagc aagaagcaga gctactgaca aaacagggtt
     5941 acactctgaa agaagatgcg ggtcgcggct atcgccgtgt cgttgcttcg ccaaaaccgg
     6001 ttgatatcat tgaaaaagaa acggttaaag ctctggtaga tgccggacag gtggtgatca
     6061 ccgttggcgg tggtggtatc cctgttattc gtgaaggtaa ccatctgcgt ggtgccagcg
     6121 cggttatcga taaagactgg gccagcgccc gtttagcaga aatgattgat gccgatatgc
     6181 tgatcattct gactgcggta gaaaaagtgg ccattaactt cggaaaagag aatgaacagt
     6241 ggctcgatcg cctgtcgtta agtgatgctg aacgctttat cgaagaaggt cattttgcga
     6301 aaggttctat gctgcctaaa gtggaagctg ccgcctcgtt tgctcgctct cgcgcaggtc
     6361 gtgaagcact gattaccgta ttgagcaaag cgaaagaagg gattgaaggc aaaaccggaa
     6421 cggtgatttg tcagtaattt aaaggccgga tgggtaatac catccggcca ttttcattta
     6481 agccacttcc agcacttctt ttgtcgcttt aacgccctga tgcatcaaca tcattaacgc
     6541 ctcaagtacg ccgccgccaa tcgcccgggc tttatcagaa acgctggtga aatcagccgt
     6601 ttcaccacga ggatcgatat caccaatttt aaaaccaccg accacggcca gtccgtcgtt
     6661 caacaagcca cgcaccatcc ccgtcaacgg tgctttaatt tcatgctcac caatccaggc
     6721 aatgacatcg ccctctttca ccagatcgcc taatttcacg ttggatcgca taatgcctgc
     6781 agcaggagca cggatcaccc gtcgggtggt atgccccata atattgccgg gaacaccggt
     6841 attctcctgc gcacaaccag agtaaatcac ctgaccgagc cagtgcccgc gatttgtttc
     6901 aattaccgca tgacaatcct tccctgcagt aaagcccggc ccaagcgcga ttgttactgg
     6961 tgccatatct gcccgcgttc ccaaattctg tttcgccaga atagcgtcca ccacgcaaag
     7021 cggtttcagt tcatcaagca atgaacaggt gggatctacc atcacaggga taaatccgcg
     7081 ttcggtaagt ttcatcgctt ccgcagagct ggttgccagg cgggcggtga cgccttcgac
     7141 cgtcatttcg ccatcgaaca cggcctgggc aaacgccacg gtacaacgaa tcactgtcgg
     7201 tttttccact tccaacatga tcactttaaa acccgcatga tacagacgta gcgctacacc
     7261 actggcgata tcccccgcac cgcgaatcac caccagctgg tgtccggaag gatgagaagg
     7321 cttcatcatc agcccacccg gcgcgttatt tttcacctgt aatatttctg ccagcacgct
     7381 gatggcgatc tcctgcggcg tttccgcacc tatgttgtaa ccaacgggcg catgtaaacg
     7441 ggcaatatgt tcttcagcca cgcctttctc acgcaattgg cgcaggaaaa gctgaacctt
     7501 gcggcgactt gccagcaacc ccagccatgc aatgggttgt tcaatgagtt tgtcgagggc
     7561 ttcacgatcc tgattattcg tggcaatcag gacaaaatta tcagggcgaa tatccagtgc
     7621 ttccactgcc gcaccaaacg actcagcatg gagaagcgtg gttgatggtg ggaatagttc
     7681 aggattgagg ctttcgcgat aaatatcagc aacggcgata tcaaatccta atagcgccgc
     7741 actctgggct atcgcccggt tgacatgccc cgcaccaatc aacaccagac gtggacgcat
     7801 accatggacg ctgataaata ctgacattgc acctccacaa tctgacccga cagcatccgc
     7861 accgttacga gccatacgtc catggaataa tcgcggctta cgttcctgca atgcctgaag
     7921 cgactcttca atcaccttcc gttcaaccat tccgccacca attgtaccga cgatagaacc
     7981 atcggcgcgc actaacattt gtgcagaatg gcggggggtt gagcctcggc tatcaacaat
     8041 ttgcgccatc gcaaacggac aattttgctc ttcgagtttt gcagcctctg tgaaaatatt
     8101 cataccaacc tcaatggata tcctttcagt aacccggaat acccgggccg ctaattcaca
     8161 aatctgcgcg cgattgcagg atgttcttgt atatcgccca gccagattgc ctctacgttg
     8221 tgttgttgca gcggttgaag gagctcgctt tgcgcaatcg cattctcaca ttgagaaaaa
     8281 cggttaatga accagactcg ccgacaacct tgcggtacgt ttttaaacgc cccctgaggg
     8341 tggcgaacca gcgcaacgag atcgctcagt tgcaagggtg catcaggtgt taacccagta
     8401 atatcagcaa actgcgacca gcgatggaca ttttctgtgc tcactttcgc acccaaaata
     8461 tgtcctccca tcacagcaat cacgcagcaa ctgcttttag gtatgcaagg ttcgtgctca
     8521 tcaggcgctt ttaacggcat tccacgcgag ccatctgcct caatgagaat tacgtcacac
     8581 tctggtcgtt gtaccagtgc atcaatcact tctggcgtaa atccctgcac ttttccctgg
     8641 ttcgctttcc agctgtgaaa acaaaatgaa atgggggata taaaagacgc atgaggaagc
     8701 atggcgggat cacgacagaa aaccacgggc cagtgagatg ttggcataaa catatgtgtc
     8761 gtcgtagtga ttaatacacg cctgccactc gcctggaaca gttctgccag ccaaaaaagc
     8821 aagctggttt tccccccagc accaacaata gaaatcacag tgggacgttt ctgcgcgcct
     8881 aaatcaatga ataatgccga tgggtcaatt atacttttca cgcgtcttta tggacctctc
     8941 tgggataaat taccctcaac gtaatccatc aggaagtaac gcaattatca ggcgttatta
     9001 gctcctgtaa ataatggaat cactatgtca gccatcgact gtataattac cgccgcagga
     9061 ttatcatcaa gaatggggca atggaaaatg atgttaccct ggcaacaggg aacaattctt
     9121 gatacaagta tcaaaaatgc gttgcagttt tgtagccgaa ttattttagt caccggctat
     9181 cgtggtaatg aactgcacga acgttatgcg aaccagagca atatcactat tatccacaac
     9241 ccagattatg cgcagggttt actgacatca gtaaaggccg cagtacccgc ggtgcaaaca
     9301 gaacattgtt ttctcaccca cggagatatg ccaaccctca ccatcgatat ttttagaaaa
     9361 atctggtcgt tacgaaatga tggcgcaata ctgccactcc ataatggcat ccccggccat
     9421 ccgattttag tgtcaaaacc atgcctgatg caggcaatcc agcgacccaa tgtcaccaat
     9481 atgcgtcagg cactccttat gggagaacat tattccgtcg aaatagaaaa tgcagaaata
     9541 attttagata ttgatactcc ggacgacttt attacagcga agaaaaggta tactgaaatt
     9601 tagaaaacgt agttaaacga ttgcgttcaa atatctaatc cctccagcac tctttatcat
     9661 aatgataagg aatcactgat tttgagaaaa gagaaaaagt gataaatggg ctggcaataa
     9721 taattttcag gagtgatttt aattctcatt tggcacctgt gagttcattc acaaaaagcc
     9781 attaagtttc gagccgcctc acattttttt atatttcccg ccaaacctgg caagagtggt
     9841 gtgattgttg ctctatcccc ctaaaccacc ggatttctca acaccggtca ctcaatgata
     9901 tctgtataag ctaaggagag ggttatgggg gatattatgc gtcccattcc gtttgaggaa
     9961 cttttgacgc gcatatttga tgaataccaa caacaacgct caatctttgg tattcccgag
    10021 caacagtttt actcacccgt aaaaggtaaa actgttagcg tcttcggtga aacctgtgcc
    10081 actcccgtcg gccctgccgc tggcccgcac acgcaacttg cgcaaaacat cgtcacctcc
    10141 tggctgactg gcggacgctt catcgaacta aaaaccgtcc aaattcttga ccgcctggag
    10201 ctggaaaagc cctgtatcga tgccgaagac gagtgcttta acaccgaatg gtctaccgaa
    10261 tttaccctgc ttaaagcctg ggatgaatac ctcaaagcct ggtttgccct gcaccttctc
    10321 gaagcgatgt tccagccttc tgattccggt aaatcgttca tctttaatat gagcgtcggt
    10381 tacaacctcg aaggtattaa gcaaccgccg atgcagcagt tcatcgacaa tatgatggac
    10441 gcatctgacc atccgaaatt cgctcaatac cgcgatgcac ttaataagct actccaggat
    10501 gaggcatttt tagcccgcca cggattgcag gaaaaacgcg aaagcttgca agccttaccc
    10561 gctcgcatcc ccaccagcat ggtacaaggg gttaccctct ccaccatgca cggctgtcct
    10621 ccgcatgaaa tcgaagccat ttgccgctac atgctggaag aaaaagggct caacaccttt
    10681 gtgaaactca acccgacctt actggggtac gcgcgtgttc gtgagatcct cgatgtctgc
    10741 ggtttcggtt acatcggctt aaaagaagag tcatttgatc acgacctcaa gctgacgcaa
    10801 gcactggaaa tgctggaacg cctgatggca ctggcaaaag aaaaatcact cggctttggc
    10861 gtaaaactga ctaacactct cggcaccatc aacaataaag gcgcattgcc cggtgaagag
    10921 atgtatatgt caggccgtgc gctgttcccg ctctccatca acgttgcagc agttctctct
    10981 cgcgcctttg acggcaaact gcccatttct tattccggtg gtgccagtca gctgactatc
    11041 cgcgatattt ttgatactgg tattcgccct attactatgg caaccgacct gctgaaacct
    11101 ggcggctatc tgcgcttaag tgcctgcatg cgcgagctgg aaggctccga cgcctgggga
    11161 cttgaccatg ttgacgtcga acgactgaac agactggcag cagacgcgtt aaccatggaa
    11221 tacacccaga aacactggaa gccagaagag cgtattgaag tggctgaaga cctgccgctg
    11281 accgattgct acgttgcccc ctgtgttact gcctgcgcta tcaagcaaga tattccggaa
    11341 tacatccgtc tgcttggcga acaccgctat gccgacgcgc tggaactcat ctatcaacgc
    11401 aacgccctgc ccgccattac cggtcatatt tgcgatcacc agtgccaata caactgtacc
    11461 cgcctggatt acgacagtgc gctgaatatc cgcgaactga aaaaagtcgc gctggaaaaa
    11521 ggttgggagg aatataagca acgctggcac aaaccagccg gttctggttc acgccatccg
    11581 gttgccgtga ttggtgcagg tccggcgggt ctggccgcag gttacttcct tgccagagcg
    11641 ggccatccgg ttacgctgtt tgaacgcgaa gccaatgcgg gcggcgtggt gaaaaatatc
    11701 attcctcagt tccgtattcc tgcagagtta attcagcacg atatcgattt tgttgccgat
    11761 cacggcgtga aatttgagta cggttgctca cccgatttga ccgttgaaca gttaaaaaat
    11821 cagggcttcc actatgttct gattgccacc ggtaccgata aaaatagcgg tgtgaaactg
    11881 gcgggcgaca accaaaatgt ctggaaatca ctccccttcc tgcgtgaata caacaagggc
    11941 accacgctca agctgggcaa acatgtggtc gttgtcgggg cgggtaacac cgcaatggac
    12001 tgcgctcgtg cggcgttacg cgttccaggc gtagaaaaag caacggtcgt ttaccgtcgt
    12061 tcactgcaag agatgccagc atggcgcgaa gagtatgaag aagcgttgca cgacggcgtg
    12121 gagttccgtt tcctgaataa tccggaacgt ttcgatgctg atggcacctt aaccttgcgc
    12181 gttatgtcgc ttggcgaacc tgatgagaaa ggtcgtcgcc gtccggttga aaccaacgaa
    12241 acagtaacac tgcatgtaga cagcctgatc accgccattg gtgaacagca ggatactgaa
    12301 gccctgaatg cgatgggcgt gccgctggac aaaaacggct ggccagacgt cgaccataat
    12361 ggcgaaacgc gtctgagtga cgtctttatg atcggcgacg tacagcgcgg accatcctcc
    12421 attgtcgctg ctgtcggaac cgcgcgtcgg gcgaccgatg ccatcctgag tcgggaaaat
    12481 atccgttccc accagaacga taaatactgg aataacgtca atccggcgga aatctatcaa
    12541 cgtaaaggcg atatctctgt cactctggtg aacagtgacg atcgtgatgc gtttgtcgcg
    12601 caggaagccg ctcgctgcct tgaatgtaac tacgtttgca gcaagtgtgt ggatgtctgc
    12661 ccgaaccgcg ccaacgtatc cattacggtc ccaggcttcc agaaccgttt ccagacgctg
    12721 cacctcgacg cttactgtaa cgaatgtggc aactgcgccc aattctgccc gtggaacggt
    12781 aaaccgtaca aagacaaaat caccgtcttc agcctgtcgc aagactttga taacagcagc
    12841 aacccaggct tccttgtgga agattgccgg gtacgcgtac gtctgaataa ccaaagctgg
    12901 gtgttaaaca tcgacagcga aggtcagttc aacaacgtac caccggagct gaacgatatg
    12961 tgccgtatca tcagccatgt ccaccagcat catcattatc tgctgggccg cgtggaggtg
    13021 taatcatgtt gattctgaag aatgtcaccg cagtgcagtt acacccggcg aaagtgcagg
    13081 aaggcgttga tatcgccatc gaaaatgatg tgattgtcgc tatcggcgat gccctgacgc
    13141 aacgctaccc cgatgccagc tacaaagaga tgcatggtcg gattgtgatg ccgggaattg
    13201 tctgctcgca taaccatttt tactcggggc tttcccgcgg aattatggca aacatcgccc
    13261 cctgcccgga tttcatctca acgctgaaaa atctctggtg gcggctcgat cgcgcccttg
    13321 atgaagagtc gctctattac agcggactga tttgttccct ggaagcgatt aagagcggat
    13381 gtacatcggt tatcgatcac catgcctctc cagcctatat cggcgtgtcg ctctccacat
    13441 tgcgcgacgc atttttaaaa gttggcctgc gcgcgatgac ctgttttgaa actactgacc
    13501 gtaacagcgg catcaaagag ttgcaggaag gtgtagaaga aaacatccgc ttcgcccgtc
    13561 agattgatga ggcgaagaaa gcagcaaccg agccgtatct ggtggaagca catatcggcg
    13621 ctcacgcgcc gtttaccgta ccggatgccg gtctggagat gctgcgtgaa gccgtgaaaa
    13681 gcacaggtcg tggtttgcat attcacgctg cggaagacct ttacgacgtt tcctacagtc
    13741 accattggta cggcaaagac ctgctggcac gactggcgca attcgatctc atcgacagca
    13801 aaacgctggt cgctcatggg ctgtacttgt cgaaagatga catcgcccta ctcaatcagc
    13861 gcgatgcgtt cctggtgcat aacgcccgtt caaacatgaa caaccatgtc ggctacaacc
    13921 atcaccttag cgacatccgc aatctggcgt tgggaacgga cggcattggt tcggacatgt
    13981 ttgaagagat gaaatttgcc ttctttaaac atcgcgatgc gggtggcccg ctgtggcctg
    14041 acagttttgc caaagcactg gctaacggca acgaactgat gagccgcaac tttggcgcga
    14101 aatttgggct tctggaagcc ggttacaaag ccgatttaac catttgcgat tacaactcgc
    14161 caacgccgct gctggcagac aatatcgccg ggcatatcgc tttcggtatg ggctcaggca
    14221 gcgttcacag cgtgatggtc aatggtgtga tggtctatga agaccgtcag tttaacttcg
    14281 attgcgattc catttatgcg caagccagaa aagccgctgc cagtatgtgg cgtcggatgg
    14341 atgcgctggc ataaataaca gctcccctct tctttcagag aagaggggaa attaaggaaa
    14401 gacgatgatt gaacaatttt tcagacccga ctctgtcgaa caagcgctgg aactgaagcg
    14461 ccgctaccag gatgaagccg tctggttcgc cgggggcagc aaactcaacg ctacaccaac
    14521 ccgtaccgat aaaaagattg ccatttcctt gcagaatctg gagctggact ggattgactg
    14581 ggataacggt gcactacgaa ttggcgcaat gtctcgcttg cagtcactgc gtgatgcacg
    14641 atttattcct gcagcactgc gtgaagctct cggttttgtt tactcacgcc atgttcgtaa
    14701 tcagtcgacc attggtggtg aaatcgccgc ccggcaggaa gagtcggtgc tgcttcccgt
    14761 cctgctggca ctggatgctg aactggtttt tggcaacggc gaaacgctgt caatcgagga
    14821 ctacctggcc tgcccatgcg atcgcctgtt aaccgaaatt atcattaaag atccgtatcg
    14881 cacctgtgcg actcgcaaaa ttagccgttc tcaggcaggt ttaaccgtcg tgacggcagc
    14941 cgttgcaatg acagaccacg acggtatgcg aattgcgctg gatggcgtcg ccagtaaagc
    15001 actgcgtctg cacgatgttg aaactcaaaa tctggaaggc aatgcacttg aacaggctgt
    15061 cgccaacgcc attttcccgc aggaagattt gcggggcagc gtggcctata aacgctatat
    15121 cacgggagtt ctggtagccg acctgtatgc cgactgccaa caggctgggg aggaagccgt
    15181 atgatcatcc actttacttt aaatggcgcg cctcaggagc taaccgttaa tccaggcgaa
    15241 aacgtgcaaa agctgttgtt taacatggga atgcactctg tacgcaacag tgatgatggt
    15301 tttgggtttg ccggttctga cgcaattatc tttaacggta atatcgttaa cgcgtccttg
    15361 cttatcgccg cacagttaga gaaggcagat attcgtaccg cagaatctct gggcaaatgg
    15421 aatgagttaa gtctggttca acaggccatg gttgatgttg gcgtggtgca gtctggttat
    15481 aacgatccag ctgcggctct gattatcacc gatcttctcg atcgcatcga cgcacctacc
    15541 cgcgaagaga tcgacgacgc actctcaggt ctgtttagcc gcgatgctgg ctggcagcaa
    15601 tactatcagg tcattgaact ggcggttgca cgtaaaaata atccgcaggc caccattgat
    15661 atcgctccga ctttccgtga cgaccttgaa gtcattggca agcattatcc taaaactgat
    15721 gccgcgaaaa tggtgcaggc gaaaccctgc tatgttgaag atcgcgtaac ggctgacgcc
    15781 tgcgtcataa aaatgttacg tagcccacac gctcacgcac tgattactca tctggatgtc
    15841 agcaaagctg aagccttacc gggcgtcgtt cacgttatta ctcacctgaa ttgcccggat
    15901 atttactata ccccgggtgg tcagagcgca ccggaaccgt caccgcttga ccgccgaatg
    15961 ttcggcaaga aaatgcgtca cgtcggcgat cgcgttgctg cggtagtagc cgaaagtgaa
    16021 gacattgcgc tcgaagcact gaagctcatc gacgttgaat atgaagtgct taagccggta
    16081 atgtcgattg acgaggcaat ggcggaagat gcgcctgtcg tgcacgatga accggtggtg
    16141 tatgttgctg gtgcaccaga tactctggaa gatgataaca gccatgcggc ccagcgcggc
    16201 gagcatatga tcatcaactt cccgatcggt tctcgcccac gcaaaaatat cgctgccagt
    16261 attcatggtc atattggcga tatggacaaa ggctttgccg atgccgatgt gatcattgag
    16321 cgaacctata actcaacgca ggcgcagcag tgcccgactg aaacacatat ctgctttacc
    16381 cgtatggacg gcgatcgtct ggtgatccac gcctccaccc aggtaccatg gcacttacgc
    16441 cgccaggtcg cgcgcctcgt gggcatgaaa caacataaag ttcatgtcat taaagaacgt
    16501 gtaggcggcg gttttggctc taaacaggac atcctgctgg aagaagtgtg cgcctgggca
    16561 acttgcgtga ccgggcgtcc ggtacttttc cgctacaccc gtgaagaaga gtttattgct
    16621 aacacctctc gtcacgtcgc gaaagtcacc gtcaaactgg gcgcgaaaaa agatggtcgc
    16681 ctgacggcag tgaagatgga tttccgcgcc aacacgggtc cttacggtaa ccactcactc
    16741 accgtaccgt gtaacggacc ggcgctgtcg ctgccgttat atccatgcga taacgtcgat
    16801 tttcaggtca ccacctacta cagcaacatt tgcccaaatg gtgcttatca gggttatggc
    16861 gcaccgaaag gtaacttcgc tatcaccatg gcattagcgg aactggctga acagttacag
    16921 atcgaccaac tggaaattat cgaacgtaac cgggtacacg aagggcaaga gctgaaaatt
    16981 ctcggtgcaa tcggtgaagg taaagcgccg acctccgttc cttccgccgc cagctgcgca
    17041 ctggaagaga tcctgcgtca gggtcgcgag atgatccaat ggtcttcacc aaaaccgcaa
    17101 aatggtgact ggcacatcgg tcgcggcgtc gccattatca tgcagaaatc gggtatcccg
    17161 gatatcgatc aggctaactg catgatcaaa ctggaatcgg atggcacctt tatcgttcat
    17221 tccggtggtg cggatattgg tactggtctg gatactgtgg tgaccaaact ggcagcagaa
    17281 gtgctgcact gccccccgca ggacgtgcat gttatctctg gtgataccga tcatgcactg
    17341 tttgataaag gcgcatatgc ctcgtccggt acttgcttct cgggtaacgc tgcgcgtttg
    17401 gcagcggaaa atctgcggga gaaaatcctg ttccacggcg cgcaaatatt gggtgagcca
    17461 gtggcagatg ttcaactagc aacgccgggc gtcgttcgcg gcaagaaagg cgaagttagt
    17521 ttcggagaga tagcccataa aggcgaaacc ggcaccggct ttggttcact ggtgggaact
    17581 ggcagttata tcacacctga tttcgccttc ccgtatggcg caaacttcgc tgaagttgcc
    17641 gtcaacacgc gtacgggtga aatccgcctg gataaattct acgccttgct ggactgcggt
    17701 acaccggtca atccagagtt agcgttggga caaatctacg gtgccactct gcgagctatc
    17761 ggccacagta tgagcgaaga gatcatttat gacgccgaag gtcacccgtt aacgcgtgat
    17821 ttacgcagtt acggcgcacc gaaaattggt gacattccgc gtgatttccg cgctgtgctg
    17881 gtgccgagcg acgataaagt cggcccattc ggggcgaaat cgatctcgga aatcggtgta
    17941 aatggcgcag ctccggcgat tgctaccgca attcacgatg cctgcggcat ctggttacgc
    18001 gaatggcatt tcacaccgga gaaaatactc accgcgctgg aaaaaatata agtaataaat
    18061 ggccgcttcg gcggcctgtt tctctgtttg aacgatgact aaatagtgac tgcgaaaatg
    18121 agataagccg ttctcaaggc gtcctgcgcc ccaaattcgt gggaatttat tttactaatt
    18181 cagatgatca aatttacttt aaaaggagtg aagggatgtc tgatataaac catgcaggtt
    18241 ctgaccttat atttgaactg gaggatcgcc ctccctttca tcaggctctc gtaggtgcca
    18301 ttacccatct gttggcaatt ttcgttccga tggtaacccc cgcgttaatc gtgggtgcgg
    18361 ccttacagct ttccgctgaa acaactgcct atcttgtttc tatggcgatg atcgcctctg
    18421 gtattggtac ctggttacaa gtaaaccgct acggcatcgt cggttctggt ttactttcaa
    18481 ttcagtcagt caatttttca tttgttacgg tcatgattgc gctgggtagc agcatgaaaa
    18541 gcgacggttt tcacgaagag ttaatcatgt cgtcgctact cggcgtctcc ttcgttggcg
    18601 catttctggt tgtcggctct tcttttatcc tgccctattt acgtcgggtt attacgccta
    18661 ccgtcagcgg cattgtggtg ctgatgatcg gcttaagtct gattaaagtt ggcattattg
    18721 attttggcgg tggttttgca gctaaaagca gcggtacgtt cggcaattac gagcatctcg
    18781 gcgttggctt attggttttg attgtggtga tcggctttaa ctgctgccgc agtccgttgc
    18841 tacgcatggg tgggatcgcc attgggctat gtgtcggcta tatcgcatcg ttatgcctgg
    18901 gcatggtgga tttcagcagt atgcgcaatt tgccgttaat caccatcccg catccgttca
    18961 aatacggctt tagttttagc ttccatcagt tcctggtggt tggcacgatt tatctgctta
    19021 gcgtgctgga agctgtcggc gatatcaccg ccacagcaat ggtttcccgc cgccccattc
    19081 agggggaaga gtatcagtcc cgactgaaag gcggcgtgct ggcagatggt ctggtttctg
    19141 ttatcgcctc cgctgtcggt tcattaccat taaccacgtt tgcgcaaaat aatggggtta
    19201 ttcagatgac tggcgtcgct tcacgttatg tcgggcgaac catcgcggta atgctggtta
    19261 tcctcggctt atttccgatg attggcggct tcttcacgac cattccctca gcagttctgg
    19321 gaggcgcaat gacgttgatg ttttccatga ttgccatcgc agggattcgc atcatcatca
    19381 ccaacggttt aaagcgccgt gaaacgctta ttgtcgccac gtctttaggt ttagggcttg
    19441 gtgtctccta cgatcccgaa atttttaaaa tattgccagc ctctatttat gtattagttg
    19501 aaaaccctat ttgtgctggc gggttaactg cgattttatt aaatattatc ctccctggtg
    19561 gctaccgaca ggaaaacgtt ctgcctggta ttacctcagc ggaagagatg gattaacagt
    19621 aaaggagtca atgatgtcag gagaacacac gttaaaagcg gtacgaggca gttttattga
    19681 tgtcacccgt acggtctata acccggaaga aattgcctct gcgctgcggt ttattgagga
    19741 tggtttatta ctcattaaac agggaaaagt ggaatggttt ggcgaatggg aagacggaaa
    19801 gcatcaaatt cctgacacca ttcgcgtgcg cgactatcgc ggcaaactga tagtaccggg
    19861 ctttgtcgat acacatatcc attatccgca aagtgaaatg gtgggggcct atggtgaaca
    19921 attgctggag tggttgaata aacacacctt ccctactgaa cgtcgttatg aggatttaga
    19981 gtacgcccgc gaaatgtcgg cgttcttcat caagcagctt ttacgtaacg gaaccaccac
    20041 ggcgctggtg tttggcactg ttcatccaca atccgttgat gcgctgtttg aagccgccag
    20101 tcatatcaat atgcgtatga ttgccggtaa agttatgatg gaccgaaacg caccggatta
    20161 tctgctcgac actgccgaaa gcagctatca ccaaagcaaa gaactgattg aacgctggca
    20221 caaaaatggt cgtctgttgt atgcgattac gccacgcttc gcccctacct catctcctga
    20281 acagatggcg atggcgcaac gcctgaaaga agaatatccg gatacatggg tacataccca
    20341 tctctgtgaa aacaaagatg aaattgcctg ggtgaaatcg ctttatcctg accatgatgg
    20401 ttatcttgat gtttaccatc agtacggcct gaccggtaaa aattgtgtct ttgctcactg
    20461 cgtccatctc gaagaaaaag agtgggatcg tctcagcgaa accaaatcca gcattgcttt
    20521 ctgtccgacc tccaaccttt acctcggcag cggcttattc aacttgaaaa aagcatggca
    20581 gaagaaagtc aaagtgggca tgggaacgga tatcggtgcc ggaacaactt ttaacatgct
    20641 gcaaacgctg aacgaagcct acaaggtatt gcaattacaa ggctatcgcc tctcggcata
    20701 tgaagcgttt tacctggcca cgctcggcgg agcgaaatct ctgggccttg acgatttgat
    20761 tggcaacttt ttacctggca aagaggctga tttcgtggtg atggaaccca ctgccactcc
    20821 gctacagcag ctgcgctatg acaactctgt ttctttagtc gacaaattgt tcgtgatgat
    20881 gacgttgggc gatgaccgtt cgatctaccg cacctacgtt gatggtcgtc tggtgtacga
    20941 acgcaactaa taataaaact ttaacatcct cgtgaggaca tcattatgtc tggagacatc
    21001 ctacaaacac cggacgcacc aaagccacag ggcgcgctgg ataattattt taaaattacc
    21061 gctcgtggca gtaccgttcg tcaggaagta ctggctggct taacgacctt tctggccatg
    21121 gtttattccg ttatcgtcgt tccgggaatg ctgggcaaag caggttttcc tcccgcagct
    21181 gtgtttgttg ccacctgtct ggtcgcgggc ttcggctcgt tgctgatggg gttatgggcc
    21241 aatttgccaa tggcgattgg ttgcgctatt tccttgacgg cgtttaccgc attcagtctg
    21301 gtactcgggc aacaaattag cgttcctgtc gcactgggcg cagtctttct gatgggcgtc
    21361 atcttcaccg ctatttccgt aaccggtgtg cgtacctgga tcttacgtaa tttgccgatg
    21421 gggatcgctc acggtacagg tatcggtatc ggcctgtttc tgctgctgat tgctgctaac
    21481 ggtgtgggaa tggttatcaa aaacccgatt gaaggcttgc cagtggcgct cggtgccttt
    21541 acctccttcc cggtgatgat gagcttactg gggctggcgg tcatcttcgg tctggaaaag
    21601 tgccgcgtac ccggcgggat cttgttggtg attattgcaa tttcgatcat cggcttaatc
    21661 tttgacccag cggtgaagta ccacggtctg gtggcaatgc caagcctgac tggcgaagat
    21721 ggtaagtctc tgattttcag cctcgatatt atgggcgcac tccagccaac tgtacttccg
    21781 agtgtactgg cattggtgat gaccgcagtg ttcgacgcca ctggcaccat ccgtgccgtc
    21841 gccggtcagg cgaatttgtt ggataaagac aaccagatca tcaacggcgg caaagccctg
    21901 accagtgact cagtaagttc aatattctcc ggcctggtgg gcgcagcgcc cgcggcggtt
    21961 tatatcgaat cagcggcagg aaccgccgcc ggggggaaaa ccggtttaac cgcaaccgtg
    22021 gttggtgtgt tgttcctgat gattctgttt ttatcaccgt tatcgtttct gatccccggt
    22081 tatgccacag ctcccgcact gatgtacgta ggtttgctga tgttaagcaa cgtctcgaag
    22141 ctggatttca acgattttat tgacgcaatg gctggcctgg tgtgtgccgt gttcatcgtt
    22201 ctgacttgta atatcgttac cggtattatg ctgggctttg taacactggt ggtgggtcgc
    22261 gtctttgcgc gcgaatggca aaagctgaat attggtacgg tgatcattac tgccgcactg
    22321 gtcgcgtttt acgcgggtgg ttgggctatc taatagattc tccgcgcctt tcaacgcgga
    22381 gttttttatc aaagaggatt cgcgccgctg gcagcaatac gctgctggcg gatacgcctg
    22441 agttctcttt cagtcagcaa ctgcaacgcc tgtgtcgggc aagattcaac gcaggctggc
    22501 ccctcttccc gctgttcaca gagatcgcat ttcacgattt gttgccgggt atcacccggc
    22561 aatgactgaa tggtgatcat cccaaacggg cacgcgctaa cgcaactctg acagccaata
    22621 caacgggcag aattggcctg caccacctgc tcgcccatcg tcagcgcccc cacggggcaa
    22681 gcgccaacac aaggggcgtt ttcacactga tggcacatca ccggcgcact gatgctatcc
    22741 agccgttgta ccttcagccg gggtaaaaag atatcggcat tcaactcctg ttctgaaggg
    22801 tgagcgacca cacaggccac ttcacaagta cggcagccaa tacagtcagc cggattaacg
    22861 ataattaacg atttcatcac gatgccttcg tatcaaacag agttaacata tcgcgcgccg
    22921 cctgccttcc tgcggccatt gcagtgacaa ccagatccgc gccatgaact gcatcaccac
    22981 cagcaaagac ttttttcaga tgcgtctggg taggtaaata cccgacgtcg ccggtttgaa
    23041 tcaggcccca tttatcgagt ttaattccgc tgccctgcaa ccacggcatg gtatgtgcct
    23101 ggaaaccaaa ggccataatg agaacatcgg cgggcaattc aaactctgaa cccgcaaccg
    23161 gacgaggacg acggcgacca tccggccccg gctcacccat agcggtacga atcaggccca
    23221 ccgccgttaa gcgtccatct tcatcacaag cgatatattg cggctgaaca ttgaactgaa
    23281 actcaacacc ttcctcgcgg gcattgacca cctctttgcg cgagcccggc atactgactt
    23341 catcacgacg atacgcacag gtcacgctgg cggcattaag gcggatggaa gtccgcaaac
    23401 aatccattgt cgtatcgcca ccgcccaata ccacaactcg cttaccttcc acatccgtca
    23461 gcggatactc tgcagactcc ggcaatccca tgagctggcg ggtatgggcg gtcaggaacg
    23521 gtaacgcctg aatgacacca ggcgcatctt catgcggcag atctgctcgc atcatcccgt
    23581 aagtccccac gccgaggaaa acagcatcat attccgccgt taattcgttg aaagagatat
    23641 cgcggccaat ttcacagtta agatgaaagt cgattcccat tgcggtgaat atctctcgcc
    23701 gctggcttaa taccgtttta tcgagtttga aaggaggaat gccaaaagtc agcataccgc
    23761 caatttctgg atggcgatca aagacatcaa cctgaactcc cgcgcgcgcc agaatatcag
    23821 cacaccctaa tcctgcaggt ccagcgccaa tcaccgccac tttttcgcta cggggaacaa
    23881 ctttgctgac atcgggacgc cagcccatcg ccagcgcagt atcggtgatg tagcgttcca
    23941 gattaccgat agagactgcg ccagagtgat ctttcaaagt acatgcacct tcacaaagac
    24001 ggtcctgtgg acataccctg ccgcagattt cgggtaagga actggtctgg tggcaaagtt
    24061 ctgccgcttc aataatcttt ccttcctgta ccagacggat gtaatccgga atagcgttat
    24121 gcagcggaca atgccagttg cagttagctt tttcggcaca ataaacacag cggtcactct
    24181 cataagtcgc ttgttgtgga tccagcccgc aatagatttc gccaaagtgg gttttccgtt
    24241 cactcgctga aattttatct gcgcctttac gcgagttaac ggggagcaac gctgcactgc
    24301 gagatggctg agcgtctgat gacgcttttc ctgctgccgt tttgcgctgg cgggccacct
    24361 ttatctgctg caacccttta tcgtccatca gccgtaacgc ctgtgttggg cagacgtcaa
    24421 tacaggcttg cgtgccggaa ctgcgctggt tacaaaggtc gcatttctgt gcaatcgtat
    24481 cgaccatctc aacgacgcca aaggggcaag cgattgcgca tcttttacaa ccaatacatt
    24541 tttgctcgtc cagttgtacg ctatcggact ggaaagtcag agcattaacc ggacaagccg
    24601 taacgcaagg ggcattgttg cagtgatggc aggccaccgg attcgcagcc tggcctttcc
    24661 ctacaacgtg gatacgcggt cgaaagtcac tgtgactcag cggccagttt tcttgattgt
    24721 gtgctaccgc acaggcaatt tcacaggcat ggcagcctat acattccgca gcttcagcag
    24781 cgataaactt attcatttgc atccctttca tttaatgagt tatgtcttct caaatttcgc
    24841 gatgcaataa acgggcatat attaggtagc atgaccgttt taattataat tttgcaactg
    24901 agttcaaatt attgcccctc aatctatgcc gctttcatta tttcaacatt gccgatcaaa
    24961 agaatgctat ccagataaaa acgattaaat gcgagagtgc gacatgccag aatgattaat
    25021 gaaatatcaa tatgagaaaa ttacagataa ttccattgtc aggttctctt ctcattttcg
    25081 tggtttaacc acgattatgt gattgcctcc gcaattccta tctctaactc cccttcctcg
    25141 caaaaactgg cactccacga gcatgtgttt agacagtttc atgaacgtaa acggttgctt
    25201 tttactctgg cgggcgaaag gagaaacact gatgagcgcc atagattccc aacttccctc
    25261 atcttctggg caagaccgcc caactgatga ggttgaccgc atattatcac taggaaagct
    25321 gatcatactc ggtctgcaac acgtccttgt catgtacgca ggtgcagtcg ctgttcctct
    25381 tatgattggt gaccgactgg gcctctcaaa agaagctatt gcgatgctca ttagctcgga
    25441 tctcttttgc tgcgggatcg tcacattatt gcaatgtatc ggtatcggcc gctttatggg
    25501 gatccgcctg ccggtgatta tgtcggtgac cttcgccgct gtaacaccaa tgatagccat
    25561 tggtatgaac ccggatatcg gcctgctggg gatattcggt gccactatcg ccgcgggttt
    25621 tatcaccaca ttattagcgc cacttatcgg tcgcttgatg cctttattcc cgccactggt
    25681 taccggtgtg gtgattactt ctatcgggct tagcatcatt caggtgggta ttgactgggc
    25741 cgccggaggt aaagggaatc cgcaatatgg taatcccgtt tatttaggta tctcctttgc
    25801 cgtcttaatt tttatcttgc tcattactcg ctatgcgaaa ggatttatgt ccaacgtcgc
    25861 cgtattactg gggattgtat ttggcttttt actttcgtgg atgatgaatg aagtcaattt
    25921 atccgggcta catgatgctt catggtttgc gattgtcacg ccgatgtcat ttggtatgcc
    25981 gattttcgat cccgtttcca ttctgaccat gactgccgtg ttaatcatcg tgtttatcga
    26041 gtcgatgggg atgttcctgg cactgggtga aatagtcggt cgtaaactct cttcacacga
    26101 tattattcgc gggctgcgtg tcgatggtgt agggacaatg ataggcggaa cgtttaacag
    26161 cttcccccac acgtcatttt ctcaaaacgt tggcctggtt agcgtgacgc gcgttcatag
    26221 ccgctgggtg tgcattgctt ccggtatcat tttgatcctg ttcggcatgg tgcccaaaat
    26281 ggcggtgctg gtggcctcga ttccgcaatt tgtgctgggc ggcgcggggc tggtgatgtt
    26341 cggcatggta ctggcgacag ggattcgaat tctgtcgcgc tgtaactaca ccactaaccg
    26401 ttacaacctc tatattgtgg cgatcagtct cggtgttggc atgacgccaa cgctctctca
    26461 cgatttcttt tctaagttac cggccgtact gcaaccgttg ctgcatagcg gcattatgct
    26521 cgcaaccctt agcgccgttg tgctgaacgt cttctttaat ggctatcagc atcatgctga
    26581 tctggtgaag gaatccgtct ctgataaaga tttaaaagtc aggacagtac gtatgtggct
    26641 tctgatgcgc aagctgaaga aaaatgagca tggagaataa tatgaatctt ttaatgcgcg
    26701 ctatattcag tctgctgttg ctttttactc tctctattcc tgtcatttct gactgtgttg
    26761 taatggccat tgaaagtcgc ttcaaatata tgatgctgct tttttaaatg gtttttacct
    26821 gtcgacatcc gctcaaaacg ggcggttgtc gataaacgct cacttggtta atcatttcac
    26881 tcttcaatta tctataatga tgagtgatta gaattacatg tgagaaatta tgcaaacgga
    26941 acacgtcatt ttattgaatg cacagggagt tcccacgggt acgctggaaa agtatgccgc
    27001 acacacggca gacactctct tacatctcgc gttctccagt tggctgttta atgccaaagg
    27061 gcaattatta gttacccgcc gcgcccttag caaaaaagcg tggcctggcg tgtggactaa
    27121 ctcggtttgt gggcacccac aactgggaga aagcaacgaa gacgcggtga tccgccgttg
    27181 ccgttatgag cttggcgtgg aaattacgcc tcctgaatct atctatcctg acttctacta
    27241 tcgcgccacc gatccgaatg gcattgtgga aaatgaagtg tgtccggtat ttgccgcacg
    27301 caccaccagt gcgttacaga tcaacgatga tgaagtgatg gattatcaat ggtgtgattt
    27361 agcagatgta ttacacggta ttgatgccac gccgtgggcg ttcagtccgt ggatggtgat
    27421 gcaggcagcc aatagtgaag caagaaaatt gttgtctgct ttcgcgcagc acaattaaaa
    27481 aaccccgaca tttgccgggg ttgtgagcat aacgtaatgc ttatttcacc ggacgcatcg
    27541 ccgggaacag aataacgtcg cggatggtat ggctgttggt gaacagcatt accatacggt
    27601 cgataccaat tcccagacct gctgtcggcg gtaagccatg ttccagtgcg gtgacgtaat
    27661 cttcatcgta gaacatcgct tcgtcgtcac ctgcatcttt cgcggcaacc tgatccagga
    27721 agcgctgcgc ctgatcttcc gcgtcattca gttcgctaaa gccgttaccg atttcacgac
    27781 caccaatgaa gaactcaaag cggtctgtga tttccgggtt aacgtcgtta cgacgcgcca
    27841 gcggagaaac ttctgccgga tattcagtaa tgaaggtcgg ctgaatcaga tgtgcttctg
    27901 ccacttcttc gaagatctcg gtaacgatac ggcccagacc ccagctcttc tcaacgtgga
    27961 tgccgataga ttcagcaatc gctttcgcag agtcgaagtt gtccagatcc gccatgtcag
    28021 tttccgggcg atatttcttg atcgcttcac gcatggtcag tttttcgaac ggtttaccga
    28081 agtccagcgt tacgtcgccg taggtcactt ccgtcttacc gagaatatcc tgtgccagag
    28141 tacggaacag cgattcggtc agctcgataa gatctttgta atctgcgtaa gccatataga
    28201 gttccatcat ggtgaactct gggttatgac gtacggaaat accttcgtta cggaagttac
    28261 ggttgatttc gaatacacgc tcgaagccac cgaccaccag acgcttgagg tgcagttccg
    28321 gcgcgatacg caggtacatg tcgagatcca gcgcgttatg gtgagtgata aacggacgcg
    28381 cagcggcacc gccagggatc acctgcatca tcggcgtttc aacttccata aagccgcgat
    28441 tcaccatgaa ctggcgaata ccagagagga tctgcgagcg cactttaaag gtgttgcggg
    28501 attcatcgtt agagatgaga tccagataac gctgacgata gcgcgcttcc tgatcctgca
    28561 agccgtggaa tttatccggc agcggacgca gtgctttggt cagcagacgc agctcggtac
    28621 agtggataga cagttcaccg gttttggttt tgaacagttt acctttcgcg ccgaggatat
    28681 cgccgaggtc ccattttttg aactgctcgt tgtaaacgcc ttccggcaga tcgtcacggg
    28741 caacgtacaa ctgaatgcga ccgccaacgt cctgcagggt aacgaaagac gctttaccca
    28801 taatacgacg ggtcatcatg cggccagcaa cggcgacttc gatgttcagc gcttccagtt
    28861 cttcgttctc tttaccgtcg aattctgcgt gcaattggtc agaggtatga tcgcgacgga
    28921 aatcgttcgg gaaggcaatc ccctgctcac gcaggttcgc cagcttctca cgacgcgttt
    28981 tcagttcatt gttaagatcg actaccgcgt cagcgccctg tgcgtgttgt tcagacatgt
    29041 tggttcctca taaccctgct ttcaaacttg cttcgataaa ttgatccagg ctgccgtcca
    29101 gcacggcctg cgtgttgcgg gtttctaccc cggtacgcag atctttaatg cgggagtcat
    29161 caaggacata agaacgaatc tggctgcccc agccgatgtc ggatttgtta tcttccatcg
    29221 cctgtttctc ggcatttttc ttctgcatct ccagttcata aagcttcgct ttcatctgct
    29281 tcatggcctg gtctttgttc ttgtgctggg aacggtcgtt ctggcactga gtcacgatcc
    29341 cggtcgggat gtgagtaata cgcaccgcag attcggtacg gttaacgtgc tgaccgcccg
    29401 cgccggatgc gcgataaacg tcgatgcgca gatccgccgg attgatttcg atatcgatat
    29461 catcatcaac ttccgggtaa acaaacgcgg agctgaacga cgtgtggcga cgaccgccgg
    29521 agtcaaacgg gctcttacgc accaggcggt gaacgccggt ttctgtacgc agccagccat
    29581 aagcgtaatc gccggagatt ttgatcgtca cggatttaat acccgccact tcaccttccg
    29641 actcttcgat gatttcagtt ttgaaaccac gcgattcagc ccagcgcaga tacatacgct
    29701 caagcatgct cgcccagtcc tgtgcttccg taccgccaga ccccgcctga atatcgaggt
    29761 agcagtcggc gctgtcatat tcgccagaga acatacggcg gaactcaagc tgcgccagtt
    29821 tttcttccag ggcgtcgagt tcagcaacgg cttcgttaaa ggtttcttcg tcgtcagctt
    29881 ctacagccag ttccagcaga ccagaaacat cttccagccc ctgtttcatt tggtcgaggg
    29941 tgtcgacaac ggcttcgagg gaggaacgct ctttacccag cgcctgtgcg cgttcgggtt
    30001 cgttccagac atccggctgt tccagctcgg cgtttacttc ttccagacgc tctttcttgg
    30061 cgtcgtagtc aaagataccc cctaagaacg tcggagcgtt ccgtgaggtc ctgaatgcga
    30121 ttatttaccg gattaatttc aaacatggtc tgatttcttt tattgagcta gtcaaaatgc
    30181 ggtgataaga gcgggattgt acccaatcca cgctcttttt tatagagaag atgacgctaa
    30241 attggccaga tattgtcgat gataatttgc aggctgcggt tgccgcgaaa ctcgttgata
    30301 tcgagcttgt aagccagttg cacttcgcgc acaccgttat ccggccagag ggcggtatcg
    30361 acattaaaag caatcccgtc cagcagtgga ccgccgccga ccggttcgac catcaccttc
    30421 aaatgacgct cgcccaccag ccgctgttgc aacagacgga aatgaccgtc aaacaacggt
    30481 tccgggaaca tctgccccca cgggccagca tcacgcaaca gctgcgccac ttccatggtc
    30541 atctctgccg cacttaatgg gccgtctgat accacttccc cttgcaataa agaaggatct
    30601 aaccactcgg taaccagttc gccaaagcgt tgctgaaaga gttcgaattt atcctcttcc
    30661 agcgacaatc ctgccgccat cgcatggccg ccaaacttga gcatcatgcc ggggtaaagc
    30721 gtgtctagcc gctccagcgc atcacgcata tgcagcccct gaatggagcg accggaacct
    30781 ttcagcgtac catcaccagc tggcgcaaag gcgataaccg gacggtgaaa acgctctttg
    30841 atacgcgatg ccagaatgcc aacaacgccc tgatgccatt cggggtgata cattgccagc
    30901 ccgccgggta gcgtgtcgcg gctgcgctcc agtttctcgc acaaggtcag ggcttcaact
    30961 tgcattccct gttcgatctc ttttcgcgtc tggtttagcg catcgagttc atttgccagc
    31021 acgcgcgctt cgccgatgtt gtcgcacagc aacagcgcca cgccaacgga catatcgtcc
    31081 agtcgcccgg cagcattgag acgtggcccc agcgcaaaac ctaaatcgct ggcggcgagt
    31141 ttttgtggat cgcgattggc cacttccagc agcgctttaa tccccggacg gcactttccg
    31201 gcacggatgc gactcatccc ctgccaggtc agaatgcgat tattagcgtc cagcggcacg
    31261 acgtccgcca ctgtccccaa cgctaccaga tccagcagtt ctgccagatt aggaattgcg
    31321 atgccgcgtt catcaaacca gccctgatcg cgcaaaaaag tgcgtaacgc cagcatcaga
    31381 taaaacgcca cgccaacgcc cgccagtgat ttcgacggga aattacagtc gcgcaagtta
    31441 gggttaatga tcgcttccgc tgcgggtaat gtgtcgcctg gcaaatggtg atcggtaaca
    31501 ataaccggaa tgcccaacga acgagcgtgt tcaacccccg catgggagga aataccgtta
    31561 tccaccgtga caattaactg cgcgccacgg gcatgagcct gatcgactac ttccggactt
    31621 aagccgtaac cgtcttcgaa acggtttgga accaggtagt cgatattgct gcaaccaagc
    31681 gagcgcatcg ccagcacgct tagagccgtg ctggtcgcac cgtcggcgtc gaaatcaccg
    31741 accacaataa tccgcgttcc ttcgcgaaaa gcgttgtaaa ggatctcaac ggccttttcg
    31801 acgccgctca gttgctgcca gggcagcatg cctttaacac tgcgttccag ttcctgcgcg
    31861 ctacgcacac cccggctggc atataaacgg cgcagcaagg gaggcaattc agcgggcaag
    31921 tcggccgttt catcgacttc tcggcgacga agttgtatct gttgtttcac gcgaattatt
    31981 taccgctggt cattttttgg tgttcgtcga ggaattcttt catgtctttc ggcggctggt
    32041 aacccggaac aagtgtgcca ttgctcagca caactgccgg agtaccgcta acgccaagct
    32101 ggacgccaag tgcgtaatgg tcggcaatat ccacgtcgca gctggctggt gcgacgcttt
    32161 tacctgccat cacatcatca aacgctttgt ttttatcttt cgcacaccag atagctttca
    32221 tttctttctc tgcatcgctg tccagcccct ggcgcgggaa agcaagataa cgcacggtga
    32281 tccccagcgc gttatagtct gccatttgct catgcagttt gtggcagtaa ccacaggtaa
    32341 tatcagtaaa cacggtgatg acgtgttttt cctgcggcgc tttataaacg atcatctctt
    32401 tttcaagcgc attcaactgc tttaacagca tcttattggt gacattgacc ggagccgtgc
    32461 cactaacgtc atacattggc ccctgaatga tatgtttacc atcatcggtg atgtacaaca
    32521 caccgctgtt agtcagaact gtcttcatgc cagctacggg cgcgggctga atatcgctgc
    32581 ttttgatgcc cattttggct aacgtttgtt gaattgccgc gtcatcagcc tgaacaaagc
    32641 ctgaaaacgc tgccaacaag gtaaataaca taaaaccttt cttcataaat tttcctgttc
    32701 ttttcagaca tcacgcccgc gggtgatgct gttgatgaag ttgtcgcaga cgctcggtag
    32761 cgacatgcgt ataaatttgc gtggtggaga gatcgctgtg gcccagtagc atctgcacca
    32821 cgcgtaaatc cgcaccatga tttaataagt gagtggcaaa agcgtgacgc aacacatgcg
    32881 gtgacagctt ttcgctgtcg atacccgcca gcacggcata atgtttaatg cggtgccaga
    32941 aggtctgtcg cgtcatctgc tgcgcacgct ggctgggaaa caacacgtca attgacacac
    33001 cattcaacag ccacggacgc ccatgttcca gataggtttc cagccagtaa accgcctctt
    33061 cacctaacgg caccaggcgc tctttgttgc ctttaccaat gacccgcacc acgccctgac
    33121 gcaggctgat atcactcatt gtcagcccga ccagttcaga gacacgcagt ccggtagcat
    33181 acaacacttc aagcatggct ttatcgcgta gctccagtgg ctgatcaatt aatggtgcct
    33241 gtaataaacg ttcgacctgc gcttcgctta aatcttttgg caaacgctgg ggcaatttcg
    33301 gtgaagcgag atgcgcactg ggatcatctt cacgaaactt ttcgcgataa agatactgga
    33361 acaatcggcg cactgcactc agcaaacgcg ctgagctggt ggctttatac ccgccctcca
    33421 gccgttctgc cagtaatgcc tgcaaatcgt cactttgcgc cgtcgccagc gtcaacccgc
    33481 ggtgatgcaa ccactccacc atcattgaca gatcgcgacg gtaagcgttc aacgtatttt
    33541 cagccagatt tttttccagc cacagagcat caagaaactg ctcgatgcgt gccagatcct
    33601 gtttcacttg cgccccttat ggtcactcat ttgatccatt atgccttatt gtgccgtgac
    33661 taaagcgatt ctgatacact agccgcaaaa gccacagcag aatcgagaag cttacgttat
    33721 gaatatgggt cttttttacg gttccagcac ctgttacacc gaaatggcgg cagaaaaaat
    33781 ccgcgatatt atcggcccag aactggtgac cttacataac ctcaaggacg actccccgaa
    33841 attaatggag cagtacgatg tgctcattct gggtatcccg acctgggatt ttggcgaaat
    33901 ccaggaagac tgggaagccg tctgggatca gctcgacgac ctgaacctcg aaggtaaaat
    33961 tgttgcgctg tatgggcttg gcgatcaact gggatacggc gagtggttcc tcgatgcgct
    34021 cggtatgctg catgacaaac tctcgaccaa aggcgtgaag ttcgtcggct actggccaac
    34081 ggaaggatat gaatttacca gcccgaaacc ggtgattgct gacgggcaac tgttcgtggg
    34141 tctggcgctg gatgaaacta accagtatga ccttagcgac gagcgtattc agagctggtg
    34201 cgagcaaatc ctcaacgaaa tggcagagca ttacgcctga tgtcactgac ggcttagcac
    34261 atatgctttg ccgtcatctt tatctttgcg tctcttgttg taacaatatc cgccgtaaat
    34321 cccgccattc ggcttcgtcc atgctgtcag ctgccagcca taaatgttgc cgttttccgc
    34381 tgtcagaacg taaacgcagc atcatgccgc tcttaatcat ccacggcgct ttgacgatgc
    34441 tccactcctg cccttgccaa cgcaaacgcc cgtccatcaa caagcgaatt tccccctggc
    34501 gagcattaat gcgccgctgg ctgcgaacgc aatcaaacac caccagcgaa agtaacacca
    34561 tccataacgg ggtgtaactg agcggccagg gcatgagtaa aataacagcg gcaaccagcc
    34621 catgaatcag caaggaaagc cactgtgcgc gccaggagac gcgcaaatca gattgccaca
    34681 ggaccacgtt cccggttccg tgtctgaatg agtcggacca tcatttccag ttctgcatcg
    34741 gccggtttac cgtgattcat cagccagtta aacaggtccg gatcgtcaca ttccagcaga
    34801 cgaataaaga tgcgtttttc gtcatcgctt aagctgtcgt actcatgttc gaaaaacggc
    34861 atgattgaaa tatcgagttc gcgcatacca cggcggcatg cccaatgaat gcgggctttg
    34921 ttgttaatgt ccatcttctt cctgtctcac gaaaatccag tacccggcta ttgtaacgtg
    34981 tttttcgact tcttttacgg gaatatcagt aaacacaatc gcgatcgcga aattaattca
    35041 caacaattca agggcttcat ttttttggaa gtcgcctcgc agaaggcaca gatcgcgtag
    35101 tgaaagcact tgcattgcct catagctctt ttaccattag ttattaatac gccgttaagc
    35161 aactcaggac tctattatgg cttttacacc ttttcctccc cgtcagccta cggcttctgc
    35221 ccgtttgcca ctgacgctga tgacgcttga tgactgggcg cttgccacta ttactggcgc
    35281 ggacagcgaa aaatatatgc aaggccaggt gacagcagat gtcagccaga tgactgaaga
    35341 tcagcacctg ctcgccgccc attgcgacgc caaaggtaaa atgtggagca atttacgtct
    35401 gttccgcgac ggcgatggct ttgcatggat tgaacggcgc agcgtgcgtg aaccgcagct
    35461 gactgaactg aaaaaatatg cggtgttctc taaagtgacc atcgcgccag acgacgagcg
    35521 tgtgctgctt ggtgttgccg gttttcaggc gcgcgccgcg ttggcaaatc tctttagtga
    35581 actaccttcg agagaaaaac aggtagtcaa agaaggcgcg accacgttgc tatggtttga
    35641 acacccggca gaacgtttcc tgatcgtaac cgatgaagct actgccaata tgctgaccga
    35701 taaactgcgc ggtgaagcgg aactgaacaa tagccaacag tggctggcat taaacattga
    35761 agcgggtttt ccggtgattg atgccgccaa cagcgggcag tttatccccc aggcgaccaa
    35821 cctccaggcg ctgggcggta tcagctttaa gaaaggctgc tataccggac aagagatggt
    35881 ggcgcgagca aaattccgtg gtgccaacaa acgcgcgctc tggttgctga agggtagcgc
    35941 cagccgactg ccggaagctg gtgaagactt agagctgaaa atgggcgaga actggcgtcg
    36001 taccggtacg gtgctggctg cggtcaaact ggaagatggt caggttgtgg tgcaggttgt
    36061 catgaataac gatatggaac cggacagcat cttccgcgtg cgtgacgatg cgaatacatt
    36121 gcgtatcgag ccactgccat attcgctgga agagtaaatc tctgtatcgc atcaggcatt
    36181 tatcgcctga tgcgacactg gtgtgtcttt tcaggcctac aaaggcacgc caattacgcc
    36241 tgcccaatat acaaatagat cgccagaaag tggcacacac taccgccgag cacaaagccg
    36301 tgccagatgg catgattgta tggaatgcgc ttgcagacgt agaaaatcac cccgagcgaa
    36361 taaaccacac cgcctaccgc cagtaaggta acgctgcccg ccgcgagctt aactgccatt
    36421 tcataaatta ccaccagcga cagccagccc atcgccagat aggtcaccag agataaaatt
    36481 ttgaatcggt gcgcgatggt cagtttaaac agaataccca gcaatgccag gctccagata
    36541 acaatcatca acccgcgcgc taacggagaa tccagcccca ccagcaaaaa cggcgtgtag
    36601 gttccggcaa tcaacagata aatggcacag tggtcaaatt tcttcagcca catttttgcc
    36661 cgttgatggg gaatggcgtg atagagcgtc gaagcgagga acagcaggat catactgccg
    36721 ccatagaggc tgtaactggt tatcgccgtg gcgctggcat tgagatccac cgcctgaacc
    36781 agtagcaaca ccaacccaac gataccaaac accagcccaa tgccgtgact gacgctgttg
    36841 gcaatttcct ctgccagcga atatccctgc ttaatgaggg gcttctgaac cataacttac
    36901 tccggaaaaa cgtacacgca catgtatacc tctccagcgt aactgagaat agttccagtg
    36961 aacacctgtt agctaaaata aattctgatt tgagattaat ctccgaaaaa tcatgaaatt
    37021 aaatgcgaaa tttcaactaa caggcgtgag ttcaatttaa aggcatttaa attcaatcac
    37081 ataaaactgt gtctggtcgg gatagatgtc ggcaatgacc tttttcagtt cagtcagggt
    37141 catattttcc tgctttgcat gtttttccgt cagcgtatcc agcgttacgg ttgaggttgc
    37201 ggtgacttca atcgtgcaaa aataaccgtc atcttcaaac cgtccgacac gaagcacatc
    37261 acccgttttg aagtgcgatt cagactcgtc gcggatggtg atggttttac gcccagccag
    37321 aatgtcatcc tggaaacgtt gaaaaaaagt gatgtcgttt ggctgcatgg taatatttcc
    37381 tgtaagaatt gactcatctg gagcctatga tagtgaaaaa actcacctta ccgaaagatt
    37441 tcttatgggg cggcgcagtt gccgctcatc aggtcgaagg cggctggaac aaaggcggca
    37501 aagggccgag catttgtgac gttttgaccg gtggcgcaca cggcgtgccg cgcgaaatca
    37561 ccaaagaagt tgtgcctggc aaatactatc caaaccatga agccgttgat ttttatggtc
    37621 actacaagga ggacatcaag ctatttgccg aaatgggctt caaatgtttt cgtacttcca
    37681 tcgcctggac gcgcattttt ccaaaaggcg atgaagctca gccaaacgaa gaagggctga
    37741 agttctacga tgatatgatc gatgaactgc tgaaatacaa catcgaaccg gtgatcaccc
    37801 tctcccactt tgaaatgccg ctgcatctgg tgcagcaata tggtagctgg accaaccgta
    37861 aagtggttga tttctttgtc cgtttcgcgg aagtggtatt tgaacgctat aagcataaag
    37921 tcaaatactg gatgaccttc aacgaaatta acaaccagcg taactggcgt gcaccgctgt
    37981 tcggttactg ctgctccggc gtggtgtata ccgagcatga aaacccggaa gagacgatgt
    38041 atcaggtgct gcatcaccag tttgtcgcca gcgccctggc ggtgaaagcc gcgcatcgca
    38101 tcaacccgga gatgaaagtc ggttgtatgc tggcgatggt gccgctctat ccttactcct
    38161 gtaacccgga cgatgtgatg tttgcccagg agtcgatgcg cgaacgttac gtctttaccg
    38221 atgtgcagtt gcgtggctat tacccgtcct atgtgttgaa cgagtgggaa cgccgcggat
    38281 ttaacatcaa aatggaagac ggcgatctgg atgtactgcg cgaaggcacc tgcgattatc
    38341 tcggcttcag ctattacatg accaacgcgg tgaaggccga aggcggcacc ggcgatgcga
    38401 tctctggttt tgaaggcagc gtaccgaacc cgtatgttaa agcgtctgac tggggctggc
    38461 agattgatcc ggttggtctg cgttacgcgc tttgcgagct gtatgagcgt taccagaagc
    38521 cgctgtttat tgtcgaaaac ggttttggcg cttacgacaa agtggaagat gatggcagca
    38581 tcaatgacga ttaccgcatt gactacctgc gcgcccatat tgaagagatg aaaaaagcgg
    38641 tgacttacga tggcgtggac ctgatgggct acacgccgtg gggctgcatc gactgtgtgt
    38701 cgttcaccac cgggcagtac agcaaacgct acggctttat ctatgtgaat aaacatgacg
    38761 acggtactgg cgatatgtcg cgttcacgta agaagagctt tgactggtac aaagaggtga
    38821 ttgccagcaa cggcgagaat ctgtaattag ccgatgtgcc tgatgtgctc cgctcatcag
    38881 gcctggcact cttttaatat attgagctcg caatatttta tattacgcct cagccgaaac
    38941 acacaaagcg cactttatta acaagcgaaa aaggcgccga agcgccttta taagatagtc
    39001 gaatcagtaa attactggta ttcgctaatc ggtacgcagg agcagaacag gttacggtcg
    39061 ccgtaaacat catccagacg tttcacggtc ggccagtatt tgtctgccac acctgccggg
    39121 aatactgcaa cttcacggct gtacggatgc gcccactcgg cgaccagttc gctctgaatg
    39181 tgcggcgcgt tcaccagcgg gttatcttcc agcggccaga caccggcttt cacctgatca
    39241 atttctgcgc ggatagccag catcgcatcg ataaagcgat ccagttccac tttgctttca
    39301 gattcagtcg gttcaaccat cagcgtaccc gccaccggga acgacatggt cggcgcgtgg
    39361 aaaccgtagt cgatcaggcg cttggcaata tccagctcgc tgatgccggt ttcttctttc
    39421 agcgggcgaa tatcgagaat acattcgtgc gccacgcgac cgtcgcgacc ggtatacagc
    39481 accgggaagg catcctgcag acggctggca atatagttgg cgttaagaat tgccacctgg
    39541 cttgcttttt tcagcccttc tgcgcccatc atgcggatgt acatccagct gattggcagg
    39601 atagaggcac taccgaacgg tgccgcagaa accgcgccct gacgggttaa catgccttcg
    39661 atttgcacca cgctatgacc cggtacaaac ggagccagat gcgctttcac accgatcggt
    39721 cccatacccg gaccaccacc gccatgcgga atgcagaaag ttttatgcag gttaaggtgg
    39781 gaaacgtccg caccaataaa gcccggcgag gtgatgccaa cctgagcgtt catgttcgcg
    39841 ccatcaaggt aaacctgacc gccgaactga tgcacgactt cacacacttc acggatcgtt
    39901 tcttcataca cgccgtgggt agaaggatag gtcaccatga tacaggagag gttatcgccc
    39961 gcctgttccg ctttcgcgcg cagatcagtc agatcgatgt tgccgttttt atcacacgcc
    40021 acaaccacca cctgcattcc tgccatatgc gcggaagccg ggttagtgcc gtgcgcagaa
    40081 gccgggatca ggcagatatc gcgatgccct tcgttgcggc tttcatgata atgacgaatc
    40141 gccagcaggc ccgcgtattc gccctgtgcg ccagagttcg gctgcataca aacggcgtcg
    40201 taaccggtca gtttcaccag ccagtcagcc agctgcgcga tcatctgctg ataaccttca
    40261 gcctgttccg gcgggcagaa cgggtgcagt tcggcaaatt ccggccaggt gatcgggatc
    40321 atctcggcgg cggcgttcag tttcatggtg caggaaccca gcgggatcat cgcctgattc
    40381 agcgccagat ctttacgctc cagcgagtgc atatagcgca tcatttcggt ttcgctgtgg
    40441 tagcgattaa acaccggatg ggtgaggatt tcgtcgtcgc gcagcatcgc agcctgaata
    40501 gagcgactgt cgtgagccac gtctttgtcc agtgtgtcga tgtccagccc gtggttatcg
    40561 cccaacagca cgctgaaaag ctgcatcacg ttttcgcgcg tggttgtttc atcaagggtg
    40621 atcccaaccg cgttcagaat atcgctacgc aggttgattt cagctgcttc ggcacgcgcc
    40681 agcacgcccg ctttgtcgac cacttccaca cacaaagtat cgaaatagtg cgcatggcgc
    40741 agcttcagac ctttttgttg caggcccgcc gccaggatat cggtcagacg gtggatgcgg
    40801 ttagcgatac gtttcaggcc aaccgggccg tgataaacgg catacaggct ggcgatgttt
    40861 gccagcagta cctgggaagt acaaatgttg gagttcgctt tctcacgacg aatatgttgc
    40921 tcgcgagtct gcatcgccat gcgcagagcg gtgttgccag ctgcatcttt cgatacaccg
    40981 ataatacggc ccggcattga gcgtttgtat tcgtctttcg ccgcaaagaa tgccgcgtgt
    41041 gggccaccgt agcccatcgg tacgccaaag cgttgcgccg aaccaaaaac aatatccgcg
    41101 ccctgtttac ctggcgcagt taacagcacc agcgccataa tatcggcggc aacgctgacc
    41161 acaattttgc gtgatttcag ttcgctaata agcgcggtgt agtcgtgaat ttcaccagta
    41221 gtgccgacct gctgtaacag cacgccgaaa acgtcctgat ggtcgagcac tttttgcgcg
    41281 tcatcgacaa tcacttcaaa accaaaggtt tcggcacgag tacggaccac atccagcgtt
    41341 tgcggatgaa cgtcagaagc aacaaagaag cggttggcat ttttcagttt gctgacgcgt
    41401 ttcgccatcg ccattgcttc ggcggcagcg gtggcttcgt caagaagaga agcagaggcc
    41461 atatccagcc cggttaaatc cagcgttacc tgctggaagt tgagcagtgc ttcaaggcgg
    41521 ccctgggaga cttcaggttg atacggagta tacgcggtat accagcccgg attttccagc
    41581 atgttacgca ggataaccgg cggtagctgc acggcggtgt aacccatgcc gatgtaagac
    41641 gtgaagcgtt tattgcgact ggcaatagcc ttgagttctg ccagtgcggc gtattcggtc
    41701 gccggtgcgc caacctgcgg cggagtcgca agctgaatat ctttcggcac aatctggccg
    41761 gtcagcgcgt ttaacgattg tgcgccaacg gcattcagca tttcttgctg ttgcgcggcg
    41821 tccggtccga tatggcgttc aataaaagcg ccgctgtttt caagctggct taacgtctgt
    41881 gtcatgagcg atggttcctg aaacgtgcag tgaattgtga acctctctcc ttgcgaagag
    41941 agtgagggtg aggcgtaaat ttcctcaccc tgatcctctc ccgcagaaga ggaataaagc
    42001 cgttactcgt cttctaacaa tgcttcgtat gcggttgcat ccagcagtga ttccagttcg
    42061 ctttcatcgc tggctttgat tttgaaaatc cagccgcctg catacggttc gctgttcacc
    42121 agttccgggg aatcgctcag tgcatcgttt accgccacga tttcaccgct tactggcgca
    42181 taaatgtctg acgccgcttt taccgattcg gcaaccgcgc agtcatcgcc cgcgctaacc
    42241 gttgcgccca cttccggtag gtcaacaaac accatatcgc ctaacagctc ctgagcgtgt
    42301 tcggtgatgc caacggtgta agtgccgtcg gcttctttac gcaaccattc gtgttctttg
    42361 ctgtatttca gttctgctgg tatgttgctc atcaatcaat ctccaaaaaa gtaaatcacg
    42421 cgacggcttt gccgttacgc acaaaaacag gtttcgtcac tttaaccggc atttcacggt
    42481 tgcgaatttg cacaatcgcc gtttcaccaa taccttccgg cacgcgcgcc agcgcaatgc
    42541 tataacccag cgttggggag aaagtaccgc tggtgataat gccttcatgc tggttgccct
    42601 gcgcatcagt aaaacgtacc ggcagttcat tacgcagcac gcctttttcg gtcatcacca
    42661 gaccaaccag tttttctgtg ccatgttcac gctgcgcttc cagagcttca cgaccgataa
    42721 agtcacgatc tgccggttcc caggcgatag tccagcccat attggcggct aaaggagaaa
    42781 tagtttcgtc catctcctgg ctatacaaat tcatgcccgc ttccagacgc agcgtgtcac
    42841 gcgcgcccag gccacatggc ttaacgccag cttccaccag cgcgcgccag aaatcggccg
    42901 ctttttcatt gggcagcgca atttcgtagc ccgcttcacc ggtgtaaccg gtggtggcaa
    42961 taaacagatc gcccgcctgc acgccaaaga acggtttcat cccttccacc gcctgacgct
    43021 gggcgtcatt aaacagtgtg gcagcttttg cctgcgcatt cggcccttgt acggcgatca
    43081 tggaaaggtc atcacgaaca gtaatttcga tgccgaaagg ttcagcgtgt tgggtaatcc
    43141 aggagaggtc tttttcgcgg gtagcggagt taacaacgag gcggaagaaa tcttcagtaa
    43201 agtagtagac gatgagatca tctatcacac cgccagaggc attcaacatc cccgagtaaa
    43261 gggctttgcc gcttttggtg agcttcgcca catcgttcgc cagcagataa cgtagaaact
    43321 cccgggtgcg gctgccgcga agatcgacga tggtcatatg tgacacatca aacattccgg
    43381 catcggtacg taccgcatga tgttcgtcga tttgcgaacc gtaatgcagc ggcatcatcc
    43441 agccgtggaa atccaccatg cgagcgccgc aaagcgtgtg ttgttcgtac aaaggagtct
    43501 gttgtgccat cttgtcctca ttgaataagc ggggctgaca acttttccat ggcgaaatta
    43561 tcaccacgaa acccagcatc ggagccactc ccggtcccca acgcaatcgt tctcttttgc
    43621 ctgaacttac caccgaaaca gactgttaac cataaggtaa aattgatcat cacattagct
    43681 tatggttaaa aaatgcaaaa atcgcaacag aataaaaaac caaaaaacac atcagtttct
    43741 atacaaagat gatgtgatga gaaagttaat ttgaataaga caatattaag cgctaaaaaa
    43801 atgtcaaaaa acactaaatc aaaaatccat aacattagaa aatataatgc gaaaacggag
    43861 gttaaattag tttatttcaa atgaggaaaa tctcccggcg aaaaaaccgg gagatgaaag
    43921 tgtgatgggt atcaaataaa cagcagaaga gaaatattta acgcagccat tcaggcaaat
    43981 cgtttaatcc cattgcctga cgaataagtt gtggcttaac gccaggaagc gtatcagcca
    44041 gtttcagacc aatatcacgc agcaattttt tcgccggatt ggtaccggaa aacagatcgc
    44101 ggaatccctg cataccagcc agcatcagcg ccgcactgtg cttacggcta cgctcatagc
    44161 gacgcaggta aatgtactgc ccgatgtctt taccctgacg atgcaaccgt ttcagttcag
    44221 caatcagctc tgcagcatcc ataaagccga gatttacccc ctgccccgcc agcgggtgaa
    44281 tagtatgcgc ggcgtcgccc accagcgcca gacggtgtgc ggcaaactgg cgcgcataac
    44341 gccccgttag cgagaacacc aggcgctcac tttcgacctt gcataagccc aggcgattat
    44401 caaaagcgat atttaacgcg cgattaaatt cgtcttcgcc tgcctgctgc atccactgcg
    44461 cttcctctgg cgacagtgac cagacaatcg agcaaagatg cggatcgcta agcggtaaaa
    44521 aggccagaat gccttcgcca tggaaaacct gccgcgccac cgcatcatgc ggttcttccg
    44581 tgcgaatggt cgccaccagc gcgtgatgct gataatccca gaaagtcagc ggaatatcgg
    44641 ctttgttgcg caaccaggaa ttagcgccgt ctgcgccaat caccagacgc gccgttaaca
    44701 tgctgccatc tttcaacgtc aggaaggttt cattttctcc ccaggcgacc tgctgtaatt
    44761 ctgcgggggc caacagagtg atatctgacg actgctgcgc tttgttccac agcgcgtagt
    44821 gaatcactga gttttcaacg atatgcccaa gatggctata gcccatgctt tgatcgtcaa
    44881 acgagatgtg accaaaactg tctttatccc acacttccat accgtgatag caacaggccc
    44941 tacgagagag aatgtcctgc cagacgccaa gacgggtgag taatttttcg ctggcggcat
    45001 tgatagccga aacgcgcagt tgtggtggtg catccgccgc cagaggttcc ggtacgcgct
    45061 gctccagtac ggcaacgcgt aagccgctac cctgtaatcc gcaggcaacc gccagcccga
    45121 ccatgccgcc gccaacaatg gctacatcaa cactttgcat tgtttattcc ttaaaaccgc
    45181 cttcaacgcg ccacccaacc aagagtgcgc tgcgccagca catcgcgtgc tggggtaaat
    45241 aattccatcg tcatcagccc gatgttgcgc ccgacaacca gcggcgtcca acggttggca
    45301 aaaagatgta caaggctgtc cgtgacgccg atggttgctt cgcgatcgct ctgtcgacgc
    45361 tgctgataac ggcacaatac gccgtaatca cccatgtctt ctccgcgctc ctgcgcctga
    45421 gtcagggttt ctgcaaggct catgacatca cgcataccga ggttaaatcc ttgcccggcg
    45481 atggggtgca gagtttgcgc cgcattcccc accagcacgg tacgatgggt aataggtttg
    45541 gcggcgcggg ttaacgccag cggataagca ctgcgtttac cggcgtgagt aattttccca
    45601 agtcgccagc caaaggccga ctggagttca cggcaaaact tctcgtcgct ccacgacagc
    45661 acctcttcgc gccgctccag tggatgacac cagaccagcg aacagcgtcc gtcagacatc
    45721 ggcaacatcg ccagcgggcc atgttgcgta aagcgttcaa aagcgcgccc ttcatgcgca
    45781 acggaagtcg caacgttcgc aatcacagcc agttgttcgt aaggatcctg ctgccagtca
    45841 acgccgcaca cggtggctaa cgctgaatgg gtgccatcag cggctaccag cacgcggccc
    45901 gtcagcgtct caccactctc cagcgtcact tcaacgtggc tctgagtacg ggcaacgtta
    45961 gccacgcgat cagggcaatg cagcgttacg ccaggtgctt tgcgcagcaa tgcaaacagc
    46021 cgttgcccga cattgtgcaa ttcgacaacc tgccccagcg ccgccagttg gtaatcttct
    46081 gcggcgaggg tgacaaatcc agcgtgacca cgatcgctga catgcacggt ggtgatggca
    46141 gttgcgcaat cagccagaga ttgccagacg ccgatgcgcg ccagttgctg acaggtaccc
    46201 gccgccagcg caatagctcg tccatcaaag cccggatgag catgtgactc tggcgcggtc
    46261 gcttcaatca aatgtactgg cagcgccccg tgacttaacc gggaaatagc cagcgccagc
    46321 gtcgcgcccg ccatgccgcc accgacgatg attacgctca ttgctttctc gcagctgcca
    46381 tcaacgcttc gatttcttcc ggctttttca ccacgctggc ggtgaggttt tcattaccgg
    46441 tttcggtaat cacaatatcg tcttcaatac gaatgccgat accgcgatac tgttctggca
    46501 cttctgcatc cggcgcaata tacagccctg gttctacggt cagtaccata cccggttcca
    46561 gaatacgcga gcgatcctga ccataaacac caacgtcatg aacatccagt cctaaccagt
    46621 ggctaaggcc atgcataaag aaaggacgat gggcgttctg agcgatcagt tcatcaacat
    46681 cacctttcag gatgccgagt tttaccagac cgctaaccat gatgcgcacc acttcaccag
    46741 tgacttcctg aatggaagtt cccggacgat acaggcgcag gctggtttca agagactcca
    46801 gcacaatgtc atagatttca cgctgagcct gagtgaattt gccgttgacc gggaaggtgc
    46861 gggtaatgtc gccagcgtaa cctttgtatt cacaacccgc gtcaatcaac accaggtcac
    46921 cgtcacgcag ttcacactcg ttttcggtgt agtgcaaaat gcagccgttt tcaccgctgc
    46981 caacaatggt gttataggaa gggtaacgcg caccgtggcg gttaaattcg tggtgaattt
    47041 cgccttccag atggtactcg aacatccccg gacggcattt ttccatcgcc cgtgtatgcg
    47101 ccagtgcggt gatttcaccc gcgcggcgaa gtacggcaat ctcttctggt gatttgaaca
    47161 ggcgcatttc atgaacaaca ggacgccagt cgatcatcgt tgccggtgcg gtgagatttt
    47221 gccgcgaacc tttacgcagt ttctcaagcg cgctgttcac gatttcatca gcatatgcat
    47281 gttcgccctg agcgtggtaa accacatcca gaccattaag tagttgataa agttgctgat
    47341 tgatttcgct gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg gcatcctggc
    47401 ctaagcgacg accaaaccag atctctgccg tcaggtcgcg aacgcggtta aacaaaacgc
    47461 tgtggttatg agtgtcatcg cttttaatca gcaccagcac cgcttccggt tcgttaaagc
    47521 cggtgaagta ccagaagtca ctgttctgac gataggggta ttcgctgtcg gcgctacgtg
    47581 ttacttctgg tgcagcaaaa atcagcgcgg cgctgccggg ttgcatttgc tctaccaggg
    47641 cctgacggcg acgctgaaac tcttgccggg atatctcact cataacactc tccttacgtt
    47701 ttttgttttt agtgtagagt cggtttttgt acttctggcg cggtcggttg cggatgagta
    47761 aaggtgtcgt ggcataacag cgcggcaaca cgaacgtatt cgatgatctc ttcaagcgac
    47821 atttcaagct cttcctgatc ttcgtcttcg tcgtaaccca actgcgcgat gttacgcaga
    47881 tcgtcgatgg cttcaccggt ttctccggtc actttgtcca gcttcggttg cgtaacgcca
    47941 agaccaagca ggaagtgatt gacccaacct gccagcgcat cagcccgatc gaaaacgctg
    48001 acatcatcgc catcaggcag ataaagctga aaaaggaagc cgtcatcctg cagggcatcg
    48061 ctggtggcag agtgcatttt acgcagtgcc tgtgccagct catgaccgaa agccatgcct
    48121 tcgttcgtca ggtcgtgaag tagcggcagc catgagctat catcgttacc gccacatatc
    48181 atcccactga ttaaaccatg catctcagca ggggtcagac ccgtcccttg ttggttcaga
    48241 tactggttca tttcgttgta accaggcatt tcgttctgta tagacataag cattcgtcgt
    48301 caaagggagg aatattcatg atatgctacc actttgggcc ctggtggacc agaaaagggc
    48361 ttgtctcttc tcatcagggt agctatagtg tcgccccttc gcagaccatg ggtctaaaga
    48421 cgaaggcagc gcagtcaatc agcaggaagg tggcatgtct gcacaacccg tcgatatcca
    48481 aatttttggc cgttcactgc gtgtgaactg cccgcctgac caaagggatg cgttgaatca
    48541 ggcagcggac gatctgaacc aacggttgca agatctgaaa gaacgcacta gagtcacaaa
    48601 tactgaacag ttggtcttca ttgccgcatt gaatatcagc tatgagttag cgcaagaaaa
    48661 agcgaagact cgtgactacg cggcaagtat ggaacagcgt attcggatgc tgcagcagac
    48721 catagaacaa gcgttacttg aacaaggtcg catcaccgaa aaaactaacc aaaactttga
    48781 atgacacttt tcggtttact gtggtagagt aaccgtgaag acaaaatttc tctgagatgt
    48841 tcgcaagcgg gccagtcccc tgagccgata tttcatacca caagaatgtg gcgctccgcg
    48901 gttggtgagc atgctcggtc cgtccgagaa gccttaaaac tgcgacgaca cattcacctt
    48961 gaaccaaggg ttcaagggtt acagcctgcg gcggcatctc ggagattccc ttcttacctg
    49021 gcaccagcca tgacgcaact accagaactc ccactgacat tatcccgaca agaaatccgc
    49081 aaaatgattc ggcaacgtcg tcgtacgtta acgccggaac aacagcagga aatgggtcaa
    49141 caagccgcta cccggatgat gacgtatccc ccagtggtga tggcgcatac ggtcgctgta
    49201 ttcctctctt ttgatggcga actcgacacc cagccactca tagaacaact ctggcgcgcc
    49261 ggtaagcgcg tatatcttcc agttttgcat ccctttagtg ccggtaactt gctgttcctg
    49321 aattaccatc cgcaaagcga actggtgatg aacaggttga agatccacga gccaaaactg
    49381 gacgtgcgcg atgtgcttcc cctttcccgg ttagatgtat tgatcacgcc gctggtggct
    49441 tttgatgaat acggtcaacg cttgggaatg ggcggtggtt tttatgatcg gaccttacaa
    49501 aactggcagc actataaaat gcaaccggtg ggttatgcgc atgattgcca gttagtagaa
    49561 aaactcccag tggagaaatg ggatattcca ctgcctgcgg tggttacacc gtcgaaagtc
    49621 tgggaatggt aagggcgata catcccgcat cgccctgatt gacatcgttg attctttgac
    49681 ctaatttagt gagtaagggt aagggaggat tgttcctccc ctgtgactga ctgttaataa
    49741 gctgggaaac ttatgagtaa cagtacaatc agtatgatga caagtcgcat cataaccctt
    49801 ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc tgccctgcca
    49861 tcgtaaatcc ccattaaata aacacaacgc attgatctga ctttgattta ttttctggag
    49921 cagactcgca aagtagaatg cgcaatgcgg caacggtgtg gagaagggat aaaaaaacgg
    49981 gcaagtcagt gacctgcccg ttaattttca gagaagggga attagtacag cagacgggcg
    50041 cgaatggtac ccggaatagc tttcattgcc tgcagcgctt tttcggcaac gtcttcgtcg
    50101 gcttcaatat caataactac ataacccatc tgggcggaag tttgtagata ttgcgcggcg
    50161 atgttgacgc cctgctcggc aaaaattttg ttgagcgcag ttagcacgcc cggacggttt
    50221 tcgtggatgt gcatcagacg acgcccaccg tgcagtggca gcgagacttc cgggaagttc
    50281 accgcagaga gcgttgagcc attgtcagaa tacttgatca atttacccgc aacttccaag
    50341 ccgatattct cctgcgcttc ctgagtcgaa ccgccaatgt gtggcgtcag aaggacgttg
    50401 tcaaattcac acagcggaga ggtaaatgga tcgctattgg tcgccggttc agtcgggaat
    50461 acgtcgattg ccgcccccgc cagatgtttg ctcgccagcg cgtcacacag cgctggaata
    50521 tccaccacag taccgcgcga agcattaatc agcagcgagc cgggtttcat tagcgaaatc
    50581 tctttcgcgc ccatcatatt tttggtggac ggattctctg gtacatgcag actcaccaca
    50641 tcgctcatat tcagcaggtc agaaagatgc tgtacctgag tggcgttgcc cagcggcagt
    50701 ttgttttcaa tatcataaaa gtaaacatac attcccagcg attcagccag aatgcccaat
    50761 tgcgtaccaa tatgaccgta gccgatgata cccagctttt tgccgcgcgc ttcaaaagaa
    50821 cccgccgcca gtttgttcca cacgccacga tgcgctttag cattggcttc cggcacgccg
    50881 cgcaatagca gcagcagttc gccaatcacc agctccgcaa cagagcgcgt atttgagaac
    50941 ggtgcgttaa ataccgggat cccgcgcttt gccgccgcat ccagatcaac ctggtttgtt
    51001 ccgatacaga aacagccaat agcgaccagt ttttctgcgg cgttgatcac gtcttcagtc
    51061 agatgggtac gggatcgcag gccgatgaag tgggcatcgc ggatggattc ttttaattgt
    51121 tcatcatcca gcgcgccttt gtgaaattcg atgttggtgt aaccagctgc acgaaggctt
    51181 tccagcgcct tttggtgcac gccttctacc agcagaaact taatcttgtc tttctccagc
    51241 gatacctttg ccatttaccc aatcctgtct tttgaaatgt tgtgtgcgga tttgcatccg
    51301 cctttcaaca tatcaaaaaa taatattgcg gcaatatgaa cgtttgcgtc gcgatgttga
    51361 ggaaatatca cgcaaaagta tttcttggat gaatatgctg gtttagaaga tttatttaga
    51421 gcaatcgaca attgccaggt aaaaacgtga cacatgtcac caaatttaat gaagagaatt
    51481 tttttaacgg gggaggttcc cccgtcagat catttcacaa tagttttgac accgtcaggt
    51541 gtgccaatca gcgcaacgtc cgcgccacgg ttagcaaaca aaccaacagt caccacacca
    51601 ggaatcgcat ttatggcgtt ttccatcgct atcgggtcaa ggatttccat gccgtggacg
    51661 tcgaggatca cgttgccgtt atcggtcact acgccctgac ggtattccgg acgaccgccc
    51721 agtttcacca gctggcgcgc cactgcacta cgtgccatcg ggataacttc tactggcagc
    51781 gggaatttgc ccagaatatc aacctgcttg gaagcgtctg caatacagat aaatttttcc
    51841 gcaaccgaag caatgatttt ttcacgggtc agcgccgcgc cgccgccttt gatcatttgc
    51901 atgtggccgt tgatttcatc tgcgccatca acgtagatgc caaggctgtc gacttcgttg
    51961 agatcaaaaa cgtgaatgcc gaggcttttc agtttttcag tggaagcatc tgaactggaa
    52021 acggcccctt caatctggcc tttcattgta ccgagtgcgt caataaagtg tgcggcggtg
    52081 gaacctgtac ctacaccaac aatggtgccg ggctgaacat actgaagtgc cgcccatcct
    52141 actgcttttt tcaattcatc ctgcgtcatg atcgtttcgc ctgtggtatg aaatttcaca
    52201 cgcattatat acaaaaaaag tgattcagac cccgttggca agccgcgtgg ttaactcatc
    52261 cataaaatat cgcgcaatgg cgggcatccc ctttcgcccc gcaaataaag catacaacgg
    52321 tctggggatg ccgctccacg gtgcaaacag gcgcaccagt tccccgttcg caagcccctg
    52381 tttacaggca aactgaggca ataacgccac gccattcaac acaaccaggg cgcgaactcg
    52441 ctgagcgaaa tgcattagcg caaatccctg atcttttaag ctgcgcgtta accactgata
    52501 caaatgttac ccgccaacgg cttcggtata tgcaacccga cacaaaattg tgtcatagtg
    52561 caggaaaaag catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat
    52621 tacaggcact ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt
    52681 gcattacaca atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc
    52741 cgttgttagt gcgtaccgta ccgccgcgcc cgacggaaca agggcaaaaa ctgttggcgc
    52801 tactacgcca ggtggagctg ctggaagaag agtggctggg cgatgaacaa accggttcga
    52861 ctccgctgct gctttcactg gcggtcaacg ccgacagtct ggcgacgtgg ttgcttccgg
    52921 cactggctcc tgtgttggct gattcgccca tccgcctcaa cttgcaggta gaagatgaaa
    52981 cccgcactca ggaacgtttg cgtcgcggcg aagtggtcgg cgcagtgagt attcaacatc
    53041 aggcgctgcc gagttgtctt gtcgataaac tcggtgcgct cgactatctg ttcgtcagct
    53101 caaaaccgtt tgccgaaaaa tatttcccta acggcgtaac gcgttcggca ttactgaaag
    53161 cgccagtggt cgcgtttgac catcttgacg atatgcacca ggcctttttg cagcaaaatt
    53221 tcgatctgcc tcctggcagc gtaccctgcc atatcgttaa ttcttcggaa gcgttcgtgc
    53281 aacttgctcg tcagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagagc
    53341 tggccagcgg cgaactgatt gacttaacgc cagggctatt tcaacgccgg atgctctact
    53401 ggcaccgctt tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttgctcgatt
    53461 atggtcacaa agttcttcgt caggattaat ccatcaaata acgcctgata gcacatatca
    53521 ggcgttatcc tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaaatctca
    53581 ggcggcaaaa aacgacgtct gaatgcattt ttttgctggc gacaaaccca cgtaaaaagc
    53641 tcaccgtaag cgcaaatacc ctcattttga ttgcgtttta cggagcaaat aatgtctaac
    53701 gtgcaggagt ggcaacagct tgccaacaag gaattgagcc gtcgggagaa aactgtcgac
    53761 tcgctggttc agcaaaccgc ggaagggatc gctatcaagc cgctgtatac cgaagccgat
    53821 ctcgataatc tggaggtgac gggtaccctt cccggtttac caccctacgt tcgtggcccg
    53881 cgtgccacta tgtataccgc ccaaccgtgg accatccgtc agtatgctgg tttctcgaca
    53941 acaaaaggag tccaacgctt tttatcgccg taacctggcc gcccaccgag gctacgactc
    54001 cgataaccca cgcgtggcgg gcgacgtcgg caaagcgggc gtcgctatcg acaccgtgga
    54061 agatatgaaa gtcctgttcg accagatccc gctggataaa atgtcggttt cgatgaccat
    54121 gaatggcgca gtactaccag tactggcgtt ttatattgtc gccgcagaag agcaaggtgt
    54181 tacacccgat aaactgaccg gcaccattca aaacgatatc ctcaaagagt atctttgccg
    54241 caacacctat atttacccgc caaaaccgtc aatgcgtatt atcgcagaca tcatcgcctg
    54301 gtgttccggc aacatgccgc gatttaatac catcagtatc agcggttacc acatgggtga
    54361 agcgggtgcc aactgcgtgc agcaggtagc atttacgctc gccgatggga ttgagtacat
    54421 caaagcagca atctccgccg ggctgaaaat tgatggcttc gtccctcgcc tgtcgttttt
    54481 cttcggcatt ggcatggatc tgtttatgaa cgccgccatg ttgcgtgcgg cacgttattt
    54541 atggagcgaa gcggtcagtg gatttggcgc acaggatccg tataacaacg ttatccgcac
    54601 caccattgat cattgtgcac atcctatgtg tcgggattat ctgcatcgct atctggaaaa
    54661 tgcgtccggc ggacatattc accacgatct tagccacgtc ttcgacttac accgtaattt
    54721 aattgcaacc ggatcgatgc tgggttaatg gtttgttccg tcacgatctt cagcaatatg
    54781 ctgaagcatc gtggcggtat aacgatgatt tctgattgaa taaagatatt cctgcatata
    54841 catcggtctg tgcggcgcat caaaatattt cagcgttgcc ggattcacca gtcgccaggc
    54901 aaaatgcggg atcaccgtca gaaaatgttc ccgctccacg gcgctgattt tggccataaa
    54961 actgtaggga cgataaataa tggttggatt gatcccacaa ggacgcatat tcgcatcaat
    55021 cattgcttcg aaattggccc gattctgaaa gcgcatttgt agccagggca gctcctgaag
    55081 cagctcttgt cgctcttcat aacggcgaga aacgagaaag cccaatcgca gcggcggtag
    55141 ttcactgata gtcaggtttt ccagttcctg aactcgtgca gatacatgct gtggggagat
    55201 attaaaatct aactgacggt caaaaagatt atcgataacg ccattttcgc tgaactcaac
    55261 tggctgggcg gtcacgcctt cgtacttatc tccgagacta atcaactgat caaaaataat
    55321 ggtcggataa gtattatcta tgcctatcac aatttctcgt gaacgacgtc ctgagttatg
    55381 gatttcatta tcaatcgcag aaagtcgttg ataaaccggg aacagttttt gatacagttc
    55441 ctgtccggct ttattgaggc tgatattatt atctttgcgg gtaaatagcg tatagccaat
    55501 ttgttcttcc agcgcggcaa tgcttttgcc aaatcgcgaa gccgtcatat ggattttttc
    55561 tgccgcccgg gcaatattat tggtttgcgc taataaaatg aaattgcgca tctttttcga
    55621 aataaaaatg tccatccccc ctctcccgca tccttgtccg cttaaccgtt tcagtcagcc
    55681 tatccttgat gaaaccgcga gcaaagatag gtgattacgt catggtttta cagaaaaagg
    55741 agacaatatc gggtaaagac attagcccga cgaatacgtc gggctacaag tattaatgtg
    55801 cagcaggtgt tttagcgggg ggttgatcca caggttctaa ctggaagact acatcgacct
    55861 gatcatcaaa ctgaatagcg gcctgctcgt aagtttcctg tgcggacacc ggtgcagcat
    55921 cagctttcat catccgcacc attgggctgg gctgatagtt ggaaacatga taacgcacgc
    55981 tatataccgg ccccagtttg cgattaaagc cattcgccag ttcctgcgcc tgatgaatcg
    56041 cgttatcaat cgctgcctta cgcgctttgt ctttataggc atccggctgc gccacgccca
    56101 gcgacacaga acgaatttcg ttaagacccg cctttagcgc gccgtccagc aaggaattca
    56161 gtttgtctaa ctgacggagc gtgacttcca ccgttctcac tgcgcggtag cctttcagga
    56221 tacttttacc atcctgataa tcataatctg gctgagtgcg taagttcgct gagctgatat
    56281 cttttttcgc gatctgattg agctcaagga aggaaatgta ttgtgcgacg cgctcatctg
    56341 cctgtttctt ggcagtagcg gcatccttcg cggccacgtt aacttcaatc gcaagagtgg
    56401 caatgtctgg caccgcatcc acgcttgccg taccggaggt gacaatatgc ggtccatccg
    56461 gcaattcgtt agcctgcgct gccatcccgc taatacccat taatgccgcc agggcgataa
    56521 ctttgaactt cacgttagtc tctccgtgtt aattgacttg cccaaaattc aggacctctg
    56581 gaggcaagct tagcgcctct gttttatttt tccatcagat agcgcctaac tgaacaaggc
    56641 ttgtgcatga gcaataccgt ctctcgccag ctgcaaggca ataaaccaca taacacatcc
    56701 cactaccaga ttgataatgc gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc
    56761 tgcgagaata gccagaccaa agaaccacag gaaagaggcg ctaattgttc cgagcgcaaa
    56821 ccaacgtttt ggttccacat caagttgccc gccaaggctg cccagcacaa caaaagtatc
    56881 caggtaaaca tgcggattca gccaggtcac tgccaacatg gtggcgataa ttttccatct
    56941 gccttgtttc aggacttcgg cactggctaa ctcaatgtta ctgctcattg ctgttttaaa
    57001 agcgccaaaa ccataccaca gcaagaaggc tacgccgccc caggtgacca gcgccagcaa
    57061 ccacggcgac tgcatcaata acgcgctgcc accaaaaatc cccgcacaaa tcaggaccaa
    57121 atcgctgata gcacaaagta aggcaatcat aatgtggtac tgacgacgaa tgccctgatt
    57181 catcacaaaa gcattttgtg gaccgagcgg taggatcata gctgcgccaa gtgcaagacc
    57241 ttgaaagtaa taagaaaaca cgttggatat tccgaattaa gtatctgatg cagagactat
    57301 atcgcgggat tgttattagc ggaaattgat aatattaatt ggcaatcaga aaaactaata
    57361 agagagaggc cagagagtat tatctggcct cagcgttgat tacgcagctt tgtcttcttt
    57421 cacccgctta aagttcacat ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa
    57481 ttcacgttta atacgctcca gcacatccca gtacacgttt tgcagatcgc cgctgttgct
    57541 ccagacgcgg accacgaaat taatcgacga tgcgccaagt tcgttcagac gcacagtcat
    57601 ttcgcgatct ttcaaaatgc gatcttcaga ctggataata tcggtcagga tctgcttaac
    57661 ctgatcgata tcggaatcat acgccacgcc aataataaat tcgttacggc gagctggctc
    57721 gcgggagaaa ttaataatat tcccggcaat aattttaccg ttcggaataa cgataatttt
    57781 accgtctgca gtacgcatgg tggtggagaa aatctgcaca ctcagcacag taccggctac
    57841 gccgccgagg tcaacatatt ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc
    57901 agcggccagg ttagacagtg acccctgcaa agccagacca acagctaaac ctgcggcacc
    57961 gagtacagca atgactgacg ctgtttgtac gcccacgcgt cccagtgctg cgattagcgt
    58021 aaaggcgata attccgtaac ggactaatgc agaaagaaaa tcagcaacag tggcatcgat
    58081 tttacgggag atcatcaggc gattcaccgc gttggaaatc atccgcgcga taatcaaacc
    58141 aacgatgatg atcgcgagtg ccgccacgat gtttactgca taacttagca gcagcgcctg
    58201 gttagccacc agccagcttc ccgcgccgtt tatgctatcg acaacattca aatcttccat
    58261 tcaatattcc ttttcaactg actcaaaatg gagaaataca ctccgccgcc ttatgacggg
    58321 cagtctgaca agggtaaaca ataaagcata attttgccaa atagatcaca gatttctcat
    58381 ctgtatcacc ttgcttttgg cgaaaaaaag acccgcagag cgggccttga gataagcaga
    58441 aaggaatatc ttacagaacg tcgatcgcgt tcagttcctg gaatgctttt tccagacgag
    58501 cgatcatcga agtctgaccg gcacgcagcc atacgcgcgg atcgtagtat ttcttgttcg
    58561 gctgatcttc gcctttcggg ttgcccagct ggccttgcag gtaagcttcg ttttctttgt
    58621 agtagttcag aacaccttcc caggttgccc attgggtatc ggtatcgatg ttcattttca
    58681 ctacgccgta gcttacggag tctttgattt cctgagcagt agaaccggaa ccaccgtgga
    58741 agacgaagtt caggctgttg tgcggcaggt tgtgtttctt agaaacatat tcctgagagt
    58801 cgcgcaggat ggtcggagtc agaacaacgt tacccggctt gtaaacaccg tgtacgttac
    58861 cgaaggacgc agcgatggtg aaacgcgggc tgattttgct cagctcggtg tatgcgtaat
    58921 caacgtcttc cggctgggtg tacagtgcag aagcgtccat gtggctgttg tccacgccgt
    58981 cttcttcacc accggtgcaa cccagttcga tttccagagt catgccgatt ttggacatgc
    59041 gctccaggta tttagagcag atttcaatgt tctcttgcag agattcttca gacaggtcaa
    59101 tcatgtgaga agagaacagc ggcttaccgg tagcagcgaa gtgtttttca cccgcgtcca
    59161 gcagaccgtc gatccacggc agcagtttct tcgcgcagtg gtcggtgtgc aggataaccg
    59221 gaacaccata atgttcagcc atctggtgaa cgtgatgcgc accagagatc gcacccagga
    59281 tagcagcgcc ctgcggaacg tcagatttca cgcctttacc agcgataaaa gaagcaccac
    59341 cgttggagaa ctgaacgata accggcgctt taactttagc tgcggtttcc agtacggcgt
    59401 tgatggagtc agtaccgacg cagtttactg ctggcagtgc gaagttgttt tcttttgcta
    59461 cctggaaaac tttctgtacg tcatcaccag taattacgcc aggttttacg aaatcaaaaa
    59521 tcttagacat gtctcttgtc ctgtatcgtc gggccttata ctcgtcatac ttcaagttgc
    59581 atgtgctgcg tctgcgttcg ctcaccccaa tcacttattt atgtaagctc ctggggattc
    59641 actctcttgt cgccttcctg caactcgaat tatttagagt atgaaaaagt gcgtgctcta
    59701 aaagcgcgct gaaacaaggg caggtttccc tgccctgtga ttttttactt cttagcgcgc
    59761 tcttcgagca tcgctactgc aggcagtact ttaccttcca cgaattcgag gaatgcgccg
    59821 ccgccagtgg agatgtagga gattttgtca gcaatgccga acaggtcgat tgctgccaga
    59881 gtgtcgccgc cgccagcgat ggagaacgct tcgctgtctg cgatagcgtt agccacgatt
    59941 tcagtacctt tgcggaagtt cgggaattcg aacacgccaa ccggaccgtt ccacagaatg
    60001 gttttcgcat tcttcaggat ttcagccagt tcctgagcgg aagcatcacc gatatccagg
    60061 atctgctcgt cagctttcac atcgttaaca gatttcaggg tagccggtgc agtttcagag
    60121 aactcggttg ctacgcgaac atcagacgga accgggatgt tgcaggtggt cagcagacgt
    60181 ttagcttcgt caaccaggtc agcttcgtac agggatttac ccacatcgtg gccttgtgcc
    60241 gcgataaagg tgttagcgat accaccgcca acgatcagct ggtcagcgat tttagacagg
    60301 gagtccagaa cggtcagttt ggtagatact ttagaaccac caacgatagc caccatcggg
    60361 cgagcaggtt ctttcagtgc tttacccagc gcgtccagtt cagctgccag cagcgggcct
    60421 gcgcacgcaa cgtcagcgaa tttaccgata ccgtgagtag aagcctgcgc gcggtgagca
    60481 gtaccgaatg cgtccattac gaacacgtca cacagtgcag cgtatttttt ggacagagtt
    60541 tcgtcatctt tcttctcgcc tttgttgaag cgaacgtttt ccagaacaac cagttcacct
    60601 tcagcaacgt caacgccgtc gaggtaatct ttaaccagac gaaccgggtt agacagtttg
    60661 tctttcaggt agttaacaac cggcagcaga gagaattctt cgttgtactc gccttcggta
    60721 ggacgaccca ggtgggaagt taccatcact tttgcgcctt gtttcagggc cagttcgatg
    60781 gtcggcagag aagcacggat acgcgcgtcg ctggttactt tcccgtcttt tactggtacg
    60841 ttcagatccg cacggataaa tacacgtttc ccagcaagat ccagatcggt catcttaatt
    60901 acagacatgg tgaatcctct cgttgattct aaaagttttg cagacgctgc ttgcgtctta
    60961 cctgaaagca acagtagcca tagctaacgt cgtgtcgagc attcggttag caaagcccca
    61021 ttcgttatcg caccagacca acgttttgat caggtgtgcg ccactgaccc gggtttgagt
    61081 gccatcgaca atggcactgt gcggatcgtg gttaaaatct acagagacca acggcaattc
    61141 cgtatagtca actataccat gaaatgcacc ttgtgctgct ttttgcagca acaggttgac
    61201 ttcattggct tttacaggtt tcttcaccgt cacgcttaaa tcgattgccg tcacatttat
    61261 ggttggcaca cgtaccgcaa tcgcctcaaa gcgatcgtta aattgcggaa aaaatcgtgt
    61321 gataccggcg gccagtttag tatcgaccgg aatgatcgac tgactggctg cccgagtgcg
    61381 acgcaggtca ggatggtatg catcaataac ctgttgatcg tgcatggcgg agtgaattgt
    61441 ggtcacagtg ccggactcaa taccgtacgc atcatctaac aatttgatga cgggaattat
    61501 gcaattcgtg gtacaggaag cgttagaaac gatgcggtgt tccgcacgaa gttgatcctg
    61561 attgacgcca taaacaacgg tcgcgtcgag atcgttactg ccaggatgtg aaaagagcac
    61621 ttttttagcc ccggcggcaa tgtgtgcttc accatgctcg cgggagccat atacgccggt
    61681 gcagtcgagg actacatcaa cgccaagttc acgccagggg agcgattgca gtgaacgttc
    61741 atgcaataca cggatggcgt catcaccaac aaaaagttga tcgcgttcct gtcgtacttc
    61801 ccatgcaaaa cggccatggc tggtgtcata tttcaacaaa tgcgccatac ccgcagcatc
    61861 cgccagttcg ttgattgcca ccacggtaat ttccgcacgg cgtccggatt catacaaagc
    61921 acgaaccaca ttacgcccga tgcgaccgaa gccatttatc gctacgcgta cggtcataga
    61981 tctcctgcaa ggttttccct gagcaaattt gccagacaga gtaatccagc aaatcgttcg
    62041 gggaaacctt acctgtcgca aactgcgact gattggttaa ttgtcgaaca tttaatcgac
    62101 tgaaacgctt cagctaggat aagcgaaacg tggaataaaa ggaatgattg tccagtcaaa
    62161 taagacaatt atctgactta cgtcacgttt tcagccccgc ccctgcggaa tttattccac
    62221 tttgcagaat cacgaataag cgtagcaaaa tcttttcgtt gtgccgtcac aaaccctgac
    62281 ttatatcaga atatgactga tgcagcgtcg atattatccg caatacgtcc gagtttcgga
    62341 gtactccaat gatgacattg ccctacccat attcttcctc cgcacgtttt tacgcgctgc
    62401 gcctgctccc cgggcaggaa gttctctccc aattacgcgc cttcgcgcaa caacaacaac
    62461 tccacgccgc gtggattgca ggttgtaccg gcagtctgac tgacgtcgcc ttacgctatg
    62521 ccgggcaaga aaacactgcc ctgctaagcg gtaagttcga agtcatcgca ttgaacggca
    62581 cgctggaaca gagtggtgaa catcttcatc tctgcgtttc cgatccacac ggcacgatgc
    62641 ttggcggtca tatgatgccc ggctgtaccg tgcggaccac gcttgaactg gtgatcggct
    62701 gccttgagga actggcgttt agccgccagt cttgcgcgct ttctggttac gacgagttgc
    62761 atatttcccc ccgttaaata actactttca gtgaggtttt cttatggcgc gtcgccactt
    62821 ttccagtcag gcattggtgt tgatcgttat ctctatcgcc atcaatatga tcggcgggca
    62881 actggccagc atggtcaaat tacctatttt tctcgactcc atcggcacac ttattagcgc
    62941 ggtactgctt ggtccggtga tcgggatgct aaccggttta ctgaccaatt tactctgggg
    63001 attgctgacc gacccgatcg ccgccgcatt tgcgcccgtc gcgatggtca tcggcctggt
    63061 cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg ccaaaagtcg ttgttagcgg
    63121 cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg ctgcgtactg cgctgttcgg
    63181 cggcgtgaca ggaagcggag ccgatttgtt cgttgcctgg atgcactcga tggggcaaaa
    63241 cctggtggag tcggtggcga ttacggtgat aggcgcgaac ctggtcgata aaattctcac
    63301 agcggtgatt gtctggcttt tactgcgtca gctgccgatt cgcaccacgc gccattttcc
    63361 ggcaatggct gccgtgcgct aaatgcatcc gtttacatcc ttaacgctgt gggcgctggc
    63421 ggcctgcacc acgcttatcc tgcccacaca gacaatattg ccagtctata gcgcggcgac
    63481 ctttttctgc cttattgcgc taaaggccac gcggcggcgg gcaaaatatg tcgtctggct
    63541 gatgttctcg ctgggggctg gattgtggct ggtgcacggt ggctggctga cagaatggct
    63601 aagcggcacg ccacgctctc cagaacggtg ggcgcatgct attacgctct ggttgcggat
    63661 tctggcgatt gtttcaacct cacagttgtg gatgcagtac gtccccgtgc aacgttttat
    63721 ccgcgctttg tttgcttctc gcctgccgcc gggcgtcgct tatctgtttg ccgggccgtt
    63781 attggtcgtt gagcagttaa aacggcaatt ggcgattatt catgaagcgc agcgcgcccg
    63841 cggcgtgccg ctggatgagg gctggtatca gcgtttacgt gcgatgcccg cgttaattat
    63901 tccgctaaca cacaatgcgc taaacgatct tgctgtccgt ggtgcggcgc tggatatgcg
    63961 ggcatttcga atcaacaata ggcgcactac gttatgggca ccagccgata gcaccctaca
    64021 acgcgtagca cgttacacca tgatattgct aatgctggct gaattcggag cgtggatatg
    64081 gttacgttag aacagtttcg ctattgcccg acacactcaa cgcatcctcc gttctgttat
    64141 gactttcact atgtcaaacc ggggatggtc gccatctttg gtgacaacgg cagtggcaaa
    64201 agtacgctgg cgcagctgat ggcgggctgg tatccggact ttctaccagg agagataacc
    64261 ggcacgggga cgcttctcgg tacgcccatt gggcgtctcc cgcttaatga acaatccgcc
    64321 accatccagc tcgtccagca atcgccctat ttgcagctct ccggctgtac ctttagcgtg
    64381 gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg ctgaaaaaga gattatggcg
    64441 cggattgacg ccgccctggc cctgaccgag tgccagccgt tgcgccatcg tcacccggca
    64501 acactctccg gtggagagac acagcgtgtg gtcatcgcct gcgccatcgc gatgcagcca
    64561 aagctcttaa ttcttgatga agcctttagc cgcctgacgc cgcaggcccg cgaaatgctg
    64621 ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc tgattattct ttttgaacgt
    64681 catcacacac ctttcctgaa ccactgccag caagcgtggc aattgcaaaa cggagcgctc
    64741 cagccactat gttaacgtta aatcagatat cttatcgctg gcctggcgcg gcaacggatt
    64801 gtctttgcga catttctctg caactcaaac aaggtgaatg gttggcgctg accggcgaca
    64861 atggcgcggg aaaatcgacg ctgttacgag taatggcagg tcttcttacg cccacagccg
    64921 gtacggttat gctgcaacaa caagcgatga aaaacctgaa aaaccgccag cgcgcggcaa
    64981 agattggcgt actgtttcag gacgcggaaa accagctttt tcacagcact gttgctgacg
    65041 agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga tgaaatcact caacgtacca
    65101 atgctgccct gcaatgctgc cagttagcag acactgccag cgcgcatccg ttagatctgc
    65161 atagtgcgca gcgacggatg gtcgccgtcg ccagcctgga agcactctct ccacccctat
    65221 tgctactgga cgagccgagt cgggattttg acgaaaattg gttgagcgtg tttgaaagct
    65281 ggctggaaaa gtgccgccag cgaggcacga gcgtcgtagc aatcagtcat gacgccgcat
    65341 ttacccggcg tcatttctct cgtgtggtgc gactggaaga tggtgtaatc aggaacgtta
    65401 atccatcaga cgatattcac ccgtagcggt catcattaag gtcaaatttg caggcagact
    65461 ctcttccagc acccggcgaa cattcggccc gtcggtacgg tcataaaagg cttctgcgtc
    65521 tgccagcgat aaccctcccg ccagcttgcg ccccaccaga cgctcccgca acgcagatgc
    65581 gggagctttg ataaagatcg agaaatcaca aaattgcgca agctggcacc acttctcgtc
    65641 gtccagcaac aaccagtttc cttcgacgat gacgagtggt gcggtaacgt gcagcgcatc
    65701 ttcaacagga tcatgctttt gtcgatcgta ctgcggccac gtacaatccc cttccacaac
    65761 ccggcacaga ttttccgcca gtttcgcaac gttgaatgtc tctggtgcgc ctttgaaggg
    65821 gcgcaattga tgcgcatcca gccagctatt gtaatggtga aaaccatcca tcggcagtgt
    65881 ctgaatagcg ggcagttccg ggtcttgttg tgcgagatat tcccagaagg tggttagggt
    65941 agatttaccc gtccccggcg gagcgcaaag aaaaaccacc gtacgccgct gcggatttac
    66001 tgtctgtaag gccgccagca tacgcagcaa cggtttatgt acattttcaa tctcttcgtt
    66061 ctggtactgt gcctgtatct tcagcccatt caccgttaat tcaattttca cggcgttaat
    66121 tcctttaagg ttgtcgttac tgccagcgtc agcgccgttt tgaccatccc gctaaaacgc
    66181 gcctctgacc agacggcgaa atcggcgaac ttcatcgctt tcaacgcgtc atagagcgtc
    66241 gcattgcggg ttatgctatt gacgttgctg ataaaatccc aggccatgtc atcggcaaat
    66301 ccgggcattt cattctgttc gttcaggtaa tgccaaaact gggaaaaatg cgtgatatcg
    66361 gcgtacagcc atttatccat tttccccagc gcataaatca aacgcagcgc aacatcaata
    66421 tcgtcaagcg ggccgctttg ggcgagtaac ggcttcactg catattcgac gatctcttca
    66481 tcgttatcga caaacagcga cggcagaaac tggcgtatcc cctggagcaa aatagaatgc
    66541 gcagttttca taaatgaaaa taaattgtcc tgtgcattca gttgctcaag cacatcatct
    66601 tctgtcagcg tcgccattgg tcttccgata gatcaccatc aatgcaggga gtctattata
    66661 ttacacgttt ggtccgcgcc gccgatcagt aatgtctgcg tccgcacccc attcgccgtc
    66721 tgttggatac cgttcaccaa ctcgccgccc gtcacgcccg tagcgctaaa gaggatatcg
    66781 ttccccctcg ccgataacca ctcgcccacg aaaagcaacg tcgtttaatg cctggcgcat
    66841 cgcggtgacc gcaaggccat caattttatt tttgtcgccg cagccggttt gcggccaggc
    66901 agccaacgct gcctgttccg taacgcgaaa taatggccac gccagggaca tcattgcgcg
    66961 gcctccccaa tataaacgcc aaattgtgcc agcagatatt tctcggcctg ttcattccag
    67021 ataccgtgcg ttttctcaac cagccacgcc agctctttaa acagcggatc ggttttgcct
    67081 ttttcggcaa aatcggcaat ggcggtcagc ggcatggtca cgccgttata aatgagtttt
    67141 ttaccgccag ggatatccgg cagattgagc acggtttctg gcaccgcatc caggccaccg
    67201 atatgggtca ccataaacga cggctgtaac tgccccgtgg cgctaagggc aatcgcctct
    67261 ttcatgtcgt ccgtggaacc gccggacgtg ccgacaatgt gcgtactgtt gtaatggacg
    67321 ttgtagaaat taaacggcac tttgaagttt ttatccgtcg gcccggcaaa gaagttcagg
    67381 cagccatctt ccgccagtaa ttcatcagcc atctcgacga ctgaaggcac cgctgcatag
    67441 acaaatacgt catcaaatcc ggcatcatcc gtcagcgccc gcagcgtctg gaccggatcg
    67501 ctcatccctt tggtattcat atacaccagc tcaatgcctt tactggccgc cagttccacc
    67561 ggcagcagct tttgtacctg cgtcaggcgt ttgtcgtcga tatcgactac caccacccgc
    67621 gccggttgta tgccgccgtt aatggcgtaa tcaatagcgc caattcccat cgggcctgca
    67681 cacgccagta gcgcaatatt gccaccaggt ttgacgccca tgcgatgctc ataaacatat
    67741 tgcgtggtgt gataattggc atgataagcg ccaataatgc agcacatcgg ttccgccagc
    67801 gaggccgcag caaaataaga gccgtgatac ggtaatacgc agcccaggtt aatggcgatt
    67861 tcgggaataa tcatataggt ggcgttaccg ccaaaatatt cgtagctgta gcccgctgaa
    67921 tatccgcctg gtaaccccat cgccggttgc aatacaaaac gctggccttt tttatatttg
    67981 ccagtgagat ttttacccac ttcgacaata accccggcac attcatgccc ggtaatgacc
    68041 ggatgatttt ctaaatcgtc ggggacgcgt ttatgttcac taccgagtaa cgccgctttc
    68101 caggtggata aacaaacgct gtcagaaatt acattcacta acagttcatt atcggtaatt
    68161 tctggcagct caaattcgcg cagacggaca tcccgcttgc cataaatagc agcaactttg
    68221 gttttcattt ttacctctgt aatcagtgtt tatgttcagc ggttaattga ttaaataaag
    68281 tgtcgagttt tggatcgcca atatagttat taatcaatat cagtggttta tccgtcacac
    68341 gtttcacgcg cccttccaga ctggcgtgag taacgacgat atccgcatcc gcaggcacgt
    68401 tttctatggc gtaatgtttt acttctattg ccagccccgc tttttccagg cgtttacgga
    68461 aggtggttgc gcccatcgca ctggagccca tccctgcatc gcaaacaaag gcaatacgct
    68521 taacacggct taacgagaat gcaccttctt gtttcatcgc cttaaccgca ttggctgact
    68581 gagcaaactc atcttcgctc ttcgtttcca ccgttttttc catcttcagt atcagcgaag
    68641 taatagcaaa ggacaccagg gtacctaccg taacaccggc aattgtcgcc aggaacgagc
    68701 ctttcggcgt cagtgccagg taagcaaaga tagaccccgg acttgggcca gccaccagac
    68761 caccgtccag taagttaaac atccaggtgc cagacatacc gcccgcaatc atggcaataa
    68821 tggtcagcgg cttcatcagc acatacggga aatacagttc gtggatccca ccgaggaagt
    68881 gaataatcat cgccccgggc gcagaacgtt tactcatccc tttaccaaac aaggtaaacg
    68941 ccagcagcag ccccagacca ggacctggat tggaggccac catgaagaag atggatttac
    69001 cgttaaccga agcctgttgc attcccagcg gataatagac gccctgatcg atcgcattat
    69061 taaggaaaag cactttcgcc ggttcattga tgactgacag caaaggtaga taacccgcat
    69121 gtaccagcgc ctcaatacac tctttgacga aagtattagc aattaacacc gccgggccga
    69181 taacttcaaa acccagcaga caaaggagca tccccgcgat acctaatgag aagttattga
    69241 taaccatctc aaaaccggca ggtatgcgtt tttccagtgc cttatcgaca tattttatga
    69301 ccagcccacc taacggtccc atgatcattg agccaaggaa catcgggatc tctgcgccga
    69361 cgatcacacc aatagtacct attccgccca tgaccgcccc gcgtttaccg ccgaccagat
    69421 gaccaccggt agaaccaatc atcacgggca ataaataggt aatcatcgga ccaacaattt
    69481 tggcgaaatg ttcattcggc agccagccgg tgggaataaa taatgccgtt ataaatcccc
    69541 aggcaataaa agcaccgata ttggggatga ccattgcagt caaaaatccc ccaaaagcct
    69601 ggacctttgc acgagcagac ttgttttcca taatattatc ctgtagagga gagagtaatt
    69661 atccgcggct gataatgtcc gctaattgtt tttctgatga tgcggttaag agttgttcga
    69721 gtatttcttc ttcgcacagt aattcactta acgcctgaat agcaccaatg tgcgaatcgg
    69781 catcggcagc agaaagtccg atgagtaatt taatcggttc gtcattcccc ggaaaataaa
    69841 cgccttgttt aagtaacgtc aaagacatcc cggttttaag cgctccacat tccggtcgcg
    69901 catgaggcat tgccacgcct ggtgcgagaa tataataagg accattgctg atggtggaat
    69961 ctttaatagc ctgaatgtaa ttctcgctga tatagttttt tgctaacagt gatgccatcg
    70021 agaaattgat agcttcctgc cagtcttttg ttgaatgtat aactgagatt gataattctg
    70081 gaaaataatc aataagccgc ataaattatc cttatttatt tttagggtac cgtccggcgg
    70141 taaaaataaa atacgcgtgc ggtttaatga aagcaattgg gtacgcgaga agaaaaaccc
    70201 catgaacaaa aataataaaa ccttaaataa caacaacatg aaattgatat ttttaaagtc
    70261 aactttgtat ttgttacgtt tatcacaaaa tcaacatttt gattgttgtt gtatttgtga
    70321 tgcatatcgc catttattta ttgaatgtcc gaaaacatac ttttttaatg caacgttttt
    70381 tcgctatacc cctttataga ttctgaattc tcacctgtcg gctattttct ccttacctca
    70441 tgtgacatat tccccacttt actcaaggaa gattcatatg aaagcacctg tttcattaac
    70501 ctcatttcgc ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct
    70561 gccatttctc tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg
    70621 aaaaatcgta aaagttgccg agccggggct gggttttaag atccctttta tggaaagcgt
    70681 agagaaaatt tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg
    70741 cgaccagcaa ccggcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc
    70801 aggcgcggtt tataccacct acaacaccat tgaagcatta aaagatcgtc tgattgtccg
    70861 tcagttgcca actcaactgg agaacgtttt cggacaatat accgccatca gcgccgtaca
    70921 ggatcgcaca aaactggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc
    70981 agtggtaatt gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc
    71041 gattgaagac cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac
    71101 ggagaaaatc caggcgcaaa tagcggtaac tcaggcccag gcggaagcag acagtaaact
    71161 agcggcagcc aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac
    71221 tattcgttta aaaagtgcgg ctgaagcaga agctatccgc ctgcgcggtg aagctctgcg
    71281 ggagaatccc ggacttgttg ccctcaccac tgctgagcgt tgggacggta aactgcccga
    71341 taccatgatc cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt
    71401 tcgcggccct tgagactgat agcaaagaca aaactgcctg atgcgctacg cttatcaggc
    71461 ctacatactt cctgcaatat gttgaatttg caaaatcttg taggacggat aaggcgttca
    71521 cgccgcatcc ggcataaaca acgcgcactt tgccaacaat ctgaagggcc gcatttgcgg
    71581 cccttctcac aaaacatctt accgattaca gcagcgcttt tgctttcgca acaacgttgt
    71641 caacggtgaa gccgaactct tcaaacagct gctctgccgg agcagattca ccgaaggtgg
    71701 tcatgccgac gatagcaccg ttcaggccaa catacttgta ccagtagtca gcaatgccag
    71761 cttccaccgc aacacgagcg gtaaccgctt tcggcagtac ggattcacgg taagccgcgt
    71821 cctgcttgtc gaatgcgtcg gtagacggca tggaaaccac gcgcgctttc acgccttcgg
    71881 cagtcagttt ttcgtaggcg gcaacagcca gttcaacttc agaaccggtc gcgatgaaga
    71941 tcagttccgg ctgaccagca cagtctttca gcacataacc accgcgcgcg atgtttgcca
    72001 gctgctcttc agtacgttcc tgctgcgcca ggttctgacg ggagaggata agcgcagtcg
    72061 ggccgtcctg acgctcaacg ccgtatttcc acgctactgc ggattcaacc tggtcacacg
    72121 gacgccatgt agacatgttc ggggtcacgc gcagagaagc gatctgttct accggctggt
    72181 gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt gtaaaccatc acctgacgct
    72241 gtttcatcag cgcagccata cgtacggcat tacgtgcgta ttccacgaac atcaggaagg
    72301 ttgaggtata cggcaggaaa ccaccgtgca gggagatacc gttagcaatc gcggtcatac
    72361 cgaactcgcg aacaccgtag tggatgtagt tacctgcagc atcttcgttg attgctttag
    72421 aaccagacca cagggtcagg ttagacggag caaggtcagc ggagccgccg aggaattccg
    72481 gcaacagcgg accgaacgct tcgatagcat tctgagacgc tttacggctg gcgattttcg
    72541 ccggattagc ctgaagttta gcaatgaact ctttcgcttt cgcgtcgaag tcagacggca
    72601 tttcgccttt catacggcgg gtaaattcag cggcttcctg cggataagct ttcgcgtaag
    72661 cagcgaattt ctcgttccat gcggactctt tcgcctggcc tgcttctttc gcatcccact
    72721 gagcatagat ttcagacggg atttcgaacg gcgcgtattt ccagcccagc tgttcgcggg
    72781 tcagggcaat ttcagcgtcg cccagcggcg caccgtggga gtcgtgggta ccggctttgt
    72841 tcggggaacc gaaaccgatg atggttttgc acatcagcag ggacggtttg tcagtcactg
    72901 cgcgcgcttc ttctactgcg cgtttgatgg atgccgcgtc atgaccgtcg atatcgcgaa
    72961 taacgtgcca gccgtaagct tcgaaacgca ttgcggtgtc gtcggtaaac cagccttcaa
    73021 cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc aatcagttta cccagcttca
    73081 gcgtacccgc cagagagcaa acttcgtggg agatgccttc catcatgcag ccgtcgccca
    73141 tgaaggcgta ggtgtagtgg tcgacgatgt cgtggcccgg acggttaaac tgcgccgcca
    73201 gcgttttttc tgcaatcgcc ataccgactg cgttggcaat accctgaccc agcggaccgg
    73261 tggtggtttc tacacctgcg gtgtaaccca cttccgggtg acccggagtt ttagagtgca
    73321 gctgacggaa gtttttcagt tcttccatcg gcagatcgta accggtgagg tgcagcaggc
    73381 tgtagatcag catggagccg tggccgttgg acagcacgaa gcggtcacgg tcagcccagg
    73441 acggattctg cgggttgtgt ttcaggaaat cacgccacag gacttcggca atgtcagcca
    73501 tacccatagg ggcacccggg tgaccggatt tggctttctg tactgcgtcc atgctcagcg
    73561 cacgaatagc attggcaagc tctttacgtg aggacatttt gactccagat cggatgatga
    73621 agggcacgcc cttaacgact tgacgacagc gcgttttggg ctacgccgga aaatttgcca
    73681 acaatttacc gcaagccgcg cgtcatgtac atggaacatc cttttgccgc ttcagaaatc
    73741 tctggatcat gctcgcatgt tgcgcaatcc actcgcccgt ccgctgcgct tttccttata
    73801 ctgagactga gcgtcgattc acctgcaaac ggcgcatttt tagaataatc ctgaccttgt
    73861 gcggaagaga aaacatgaaa attcgcgcct tattggtagc aatgagcgtg gctacggtgc
    73921 tgaccggttg ccagaatatg gactccagtg gactgctctc atcaggagcg gaagcttttc
    73981 aggcttacag tttgagtgat gcgcaggtga aaaaactgag cgatcaggca tgccaggaga
    74041 tggacagcaa agcgacgatt gcgccagcca atagcgaata cgctaaacgt ctgacaacta
    74101 ttgccaatgc gctaggcaac aatatcaacg gtcagccggt gaattacaaa gtgtatatgg
    74161 cgaaggatgt gaacgccttt gcaatggcta acggctgtat ccgcgtctat agcgggctga
    74221 tggatatgat gacagataac gaagtcgaag cggtgctcgg tcacgaaatg gggcacgtgg
    74281 cattaggcca tgtgaagaaa ggaatgcagg tagcacttgg tacaaacgca attcgcgttg
    74341 cggcggcctc tgcaggcgga attgtcggca gtttgtcaca atcacaactg ggagacctgg
    74401 gcgaaaagct agtcaattcg caatttaccc aacgtcagga atcagaggcc gatgattact
    74461 cctacgactt gctgcgccaa cgcggtatca gcccggcagg tcttgccacc agctttgaaa
    74521 aactggcgaa actggaagaa ggccgccaaa gctcaatgtt tgacgaccat cctgcatccg
    74581 ccgaacgcgc ccagcatatt cacgatcgta tgagcgcgga tggagttaag taaagccagg
    74641 tagtgccgaa cgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tgacacatca
    74701 cccgtgcgcc tcttatctgg cgcttactcg ccctttttcg ccgcctgaat atacagcatc
    74761 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
    74821 acttccacta cgtccatccc aacgatgttg agatctttca agccgcgtac cagtttaatg
    74881 gcgcgatcgg aggtcaggcc gccaattacc ggcgtaccgg tgcctggtgc aaaagcagga
    74941 tccaggcagt cgatatcaaa ggtcaggtaa accggcatat cacccacaat ctgtttcacc
    75001 tgggcgataa tgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
    75061 ccgttgtctt tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc
    75121 agaccttctt tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttagcg
    75181 taggtatcgg tgtgggcatc aaagtgtacc aacgccattt tgccgaaatg cttcgcatga
    75241 gcgcgcagca gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg
    75301 gcagccagca gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg
    75361 aaggcatata ccagatcacc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc
    75421 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat agctgccgga
    75481 ccgtggcgtc caccggcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
    75541 cagtcagcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg
    75601 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
    75661 caaagtcgat ataaaaaaac ccctccgcgt cgttaggccc gacgaggaag ggttggattt
    75721 gcgaaacaca agtttgtgct aattatcgcc ctaaatgggc aaggcatcaa gcaaggttac
    75781 tatttccgac acggtggcgg cgcatagcca ttgttatctg ccggaaacgc ttgtccagag
    75841 cacggcggca gcgcgttttg cgccgggcct tgtaattcaa aggcggcaaa gccgattaca
    75901 ccggtattac gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg
    75961 aactgaaaag ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taacgaatct
    76021 ccggcattca caatataacc gttgttattc agcgaacctt gcttgccgtt cagcacatcc
    76081 aggccatcaa ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggacatat
    76141 aattgataat tcattccgac tctggcatgg acaatatagc gtggttcatt attggatgaa
    76201 tcataaaccc gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt
    76261 tcgttaatac gaatgctata aacgcggtcg tagccggaag gaacattagt cgaataacta
    76321 ataacgatca tctctgccgg atcgcgccag gctcgttcag cagatacgcc ctgaaccgtt
    76381 gagtgcacat ccccacccca ggcggtgccg acattgcctg atgcactgct attatactgc
    76441 gcattactgg aacaaccggc cagcagcacg gctaatgcta tcaacgcgct acgtactttc
    76501 cattttttca taaccgttcc ctctgcattt aattaatgca gcgattgtag ggggattatt
    76561 taataagaaa agcagctaaa ttatttttaa gaatatataa aaagaaagcg ggccgattta
    76621 cgtaaataaa tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataggtata
    76681 accgtacaaa cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc
    76741 ggttttcttc acttgatcac ggaactgggt taacaacgtt ttaggatcga gctgtacata
    76801 ttgcagcata tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctgccgtc
    76861 agggaagacg aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccgag
    76921 gatctcctga tatgcgccga ccatgaagaa accgagcatc ggcggattct ccggatcgta
    76981 ctccggcata ggcattgtcg tggcaatacc gtcaccatca atatagtggt cgatagcacc
    77041 gtctgagtca caggtaatat ccagcagcac agcgcggcgt tccggcactt gatccagccc
    77101 ttccagcggc agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa
    77161 cagcgagaag ttgacgtaca ttttgtccgc catacgttcc tgcagctcgt cgataatcgg
    77221 acgatgagca cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcaaata
    77281 aagctgctca gcccatgcgc gttcttgcag gctaaaggtg ccggaagagt agccgatatg
    77341 aatgtcgtgc agatccatct ggctgtcgtg taaccattca cgcagagaac ggcgagttcc
    77401 cggttcgtgc atttcctgcc aggtttccca catgctttgc agggcgcgcg gtgcgtcttc
    77461 tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tcgacaccag
    77521 cacggtgtga tgcgcagtca ccgcacgacc cgattcggtg attaccgtcg gatgtggcag
    77581 accattttct tcacacgcat caccaatcgc ccagatgatg ttgttggcgt attcattgag
    77641 gccgtagttt acggagcagt cagactgaga acgggtgcct tcatagtcca cgcccagacc
    77701 gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg
    77761 cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg
    77821 gaagtgcagc agttgcaggc tgtcgagacg cccggcttca cgcagcgttt caaccagttg
    77881 cagtacttga gtcgccgcca ggccaaactt cgatttttcc ccgccggagg actgccattt
    77941 acccgagcct tgcgacgcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg
    78001 ttctgcttca tccagcacaa tggcaatttc tgacatcttc tcaatgacca gatagacctt
    78061 gtggcccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtcct tataaccgtt
    78121 gcagacgatg acgctacggg tcatgccagc atgtgccagc accgccatca actcagcttt
    78181 ggaaccggct tccaggccca gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg
    78241 gtgctggtta actttgatcg gataaacgag gaagtaatcg ccgttatagc cgtaggattc
    78301 ccgcgctcgt ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa
    78361 acagaacagt gcaggcagac gctgaccctg tgcttcgcga gttttcacta actgcgcgag
    78421 atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt
    78481 aacgtcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc
    78541 ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga
    78601 cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt
    78661 gtaaaacagt taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga
    78721 ttttccagca gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta
    78781 ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt
    78841 tttacaagag cgcccttgtt cagtcctcag tagctgtaac cagctcttga atcctgagaa
    78901 gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac
    78961 gaatcttcac cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat
    79021 cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgttt tcaacccggc
    79081 tggaagtggc aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt
    79141 tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgcca gcattgtcag
    79201 gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatt
    79261 gggtgaagaa ctgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac
    79321 tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc acataaaatc
    79381 cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc
    79441 tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg
    79501 atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct
    79561 acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca
    79621 tcgaagagat cacccgtaat accgttcgcg aaattggcta tgtgcattcc gacatgggct
    79681 ttgacgctaa ctcctgtgcg gttctgagcg ccatcggtaa acagtctcct gacatcaacc
    79741 agggcgttga ccgtgccgat ccgctggaac agggcgcggg cgaccagggt ctgatgtttg
    79801 gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacttat gcacaccgtc
    79861 tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg
    79921 cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggcatt gatgctgtcg
    79981 tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg
    80041 aagagatcat caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca
    80101 tcaacccgac cggtcgtttc gttatcggtg gcccgatggg tgactgcggt ctgactggtc
    80161 gtaaaatcat cgttgatacc tacggcggca tggcgcgtca tggtggcggt gcattctccg
    80221 gtaaagatcc atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa
    80281 acattgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg
    80341 tggctgaacc gacttccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac
    80401 aactgactct gctggtacgt gaattcttcg acctgcgccc atacggtctg attcagatgc
    80461 tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac
    80521 atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg tgatgctgcc ggtctgaagt
    80581 aatctttctt cacctgcttt caaaggccag cctcgcgctg gcctttttct tttacctcac
    80641 cgcggtggac aggcattcac gccgcatccg gcaaaaaaac cgcccgcaca ataacatcat
    80701 tcttcctcaa cacgttttac cgcaaattat catcactact gaaaccgatt acaccaacca
    80761 cgccagccaa atatttgtaa tattttca
//
LOCUS       DAZFRE010000009        80267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFRE010000009 DAZFRE010000000
VERSION     DAZFRE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.009"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            163..705
                     /gene="yehD"
                     /locus_tag="ACHKUI_000727"
     CDS             163..705
                     /gene="yehD"
                     /locus_tag="ACHKUI_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6596986.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
     gene            786..1460
                     /gene="yehC"
                     /locus_tag="ACHKUI_000728"
     CDS             786..1460
                     /gene="yehC"
                     /locus_tag="ACHKUI_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6596987.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKVTFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1476..3956
                     /gene="yehB"
                     /locus_tag="ACHKUI_000729"
     CDS             1476..3956
                     /gene="yehB"
                     /locus_tag="ACHKUI_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6596988.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVSNI
                     RLEDSQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDSFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLQEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGHQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3972..5006
                     /gene="yehA"
                     /locus_tag="ACHKUI_000730"
     CDS             3972..5006
                     /gene="yehA"
                     /locus_tag="ACHKUI_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO6596989.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(5088..5426)
                     /gene="rcnB"
                     /locus_tag="ACHKUI_000731"
     CDS             complement(5088..5426)
                     /gene="rcnB"
                     /locus_tag="ACHKUI_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6596990.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5645..6469)
                     /gene="rcnA"
                     /locus_tag="ACHKUI_000732"
     CDS             complement(5645..6469)
                     /gene="rcnA"
                     /locus_tag="ACHKUI_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6596991.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6590..6862
                     /gene="rcnR"
                     /locus_tag="ACHKUI_000733"
     CDS             6590..6862
                     /gene="rcnR"
                     /locus_tag="ACHKUI_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6596992.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6954..7050
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7085..7873
                     /gene="thiM"
                     /locus_tag="ACHKUI_000734"
     CDS             7085..7873
                     /gene="thiM"
                     /locus_tag="ACHKUI_000734"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6596993.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7870..8670
                     /gene="thiD"
                     /locus_tag="ACHKUI_000735"
     CDS             7870..8670
                     /gene="thiD"
                     /locus_tag="ACHKUI_000735"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6596994.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8735..9553
                     /gene="yegX"
                     /locus_tag="ACHKUI_000736"
     CDS             8735..9553
                     /gene="yegX"
                     /locus_tag="ACHKUI_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6596995.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERINWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            9605..10351
                     /gene="yegW"
                     /locus_tag="ACHKUI_000737"
     CDS             9605..10351
                     /gene="yegW"
                     /locus_tag="ACHKUI_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6596996.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10325..11290)
                     /gene="yegV"
                     /locus_tag="ACHKUI_000738"
     CDS             complement(10325..11290)
                     /gene="yegV"
                     /locus_tag="ACHKUI_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6596997.1"
                     /translation="MSGARLHTLLPELTSRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11287..12291)
                     /gene="yegU"
                     /locus_tag="ACHKUI_000739"
     CDS             complement(11287..12291)
                     /gene="yegU"
                     /locus_tag="ACHKUI_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6596998.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNTIDPALKAELDTVNQLDFNRYATALAKYRQQREAI
                     "
     gene            complement(12288..13565)
                     /gene="yegT"
                     /locus_tag="ACHKUI_000740"
     CDS             complement(12288..13565)
                     /gene="yegT"
                     /locus_tag="ACHKUI_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6596999.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13822..14874
                     /gene="fbaB"
                     /locus_tag="ACHKUI_000741"
     CDS             13822..14874
                     /gene="fbaB"
                     /locus_tag="ACHKUI_000741"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6597000.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15101..15955
                     /gene="gatY"
                     /locus_tag="ACHKUI_000742"
     CDS             15101..15955
                     /gene="gatY"
                     /locus_tag="ACHKUI_000742"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6597001.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLNHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15984..17246
                     /gene="gatZ"
                     /locus_tag="ACHKUI_000743"
     CDS             15984..17246
                     /gene="gatZ"
                     /locus_tag="ACHKUI_000743"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6597002.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLGIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            17256..17708
                     /gene="gatA"
                     /locus_tag="ACHKUI_000744"
     CDS             17256..17708
                     /gene="gatA"
                     /locus_tag="ACHKUI_000744"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6597003.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALITRE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17739..18023
                     /gene="gatB"
                     /locus_tag="ACHKUI_000745"
     CDS             17739..18023
                     /gene="gatB"
                     /locus_tag="ACHKUI_000745"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6597004.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGNIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            18027..19382
                     /locus_tag="ACHKUI_000746"
     CDS             18027..19382
                     /locus_tag="ACHKUI_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6597005.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIMFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            19430..20470
                     /gene="gatD"
                     /locus_tag="ACHKUI_000747"
     CDS             19430..20470
                     /gene="gatD"
                     /locus_tag="ACHKUI_000747"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6597006.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSLEMSAP
                     QMQGVLRELRFNQLILEAAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            20576..21349
                     /locus_tag="ACHKUI_000748"
     CDS             20576..21349
                     /locus_tag="ACHKUI_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6597007.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21431..22330)
                     /gene="yegS"
                     /locus_tag="ACHKUI_000749"
     CDS             complement(21431..22330)
                     /gene="yegS"
                     /locus_tag="ACHKUI_000749"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6597008.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMRLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASAWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            22745..23062
                     /gene="yegR"
                     /locus_tag="ACHKUI_000750"
     CDS             22745..23062
                     /gene="yegR"
                     /locus_tag="ACHKUI_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6597009.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVKLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(23391..24752)
                     /gene="yegQ"
                     /locus_tag="ACHKUI_000751"
     CDS             complement(23391..24752)
                     /gene="yegQ"
                     /locus_tag="ACHKUI_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6597010.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24899..25231)
                     /gene="yegP"
                     /locus_tag="ACHKUI_000752"
     CDS             complement(24899..25231)
                     /gene="yegP"
                     /locus_tag="ACHKUI_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6597011.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            complement(25411..26133)
                     /gene="baeR"
                     /locus_tag="ACHKUI_000753"
     CDS             complement(25411..26133)
                     /gene="baeR"
                     /locus_tag="ACHKUI_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6597012.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDCLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(26130..27533)
                     /gene="baeS"
                     /locus_tag="ACHKUI_000754"
     CDS             complement(26130..27533)
                     /gene="baeS"
                     /locus_tag="ACHKUI_000754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6597013.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            complement(27530..28945)
                     /gene="mdtD"
                     /locus_tag="ACHKUI_000755"
     CDS             complement(27530..28945)
                     /gene="mdtD"
                     /locus_tag="ACHKUI_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6597014.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKTVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSVQ"
     gene            complement(28946..32023)
                     /gene="mdtC"
                     /locus_tag="ACHKUI_000756"
     CDS             complement(28946..32023)
                     /gene="mdtC"
                     /locus_tag="ACHKUI_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6597015.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNKGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGAVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            complement(32024..35146)
                     /gene="mdtB"
                     /locus_tag="ACHKUI_000757"
     CDS             complement(32024..35146)
                     /gene="mdtB"
                     /locus_tag="ACHKUI_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6597016.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(35146..36393)
                     /gene="mdtA"
                     /locus_tag="ACHKUI_000758"
     CDS             complement(35146..36393)
                     /gene="mdtA"
                     /locus_tag="ACHKUI_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6597017.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSATPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            36672..36728
                     /locus_tag="ACHKUI_000759"
     CDS             36672..36728
                     /locus_tag="ACHKUI_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6597018.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            37000..37056
                     /locus_tag="ACHKUI_000760"
     CDS             37000..37056
                     /locus_tag="ACHKUI_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6597019.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            37274..37933
                     /gene="yegL"
                     /locus_tag="ACHKUI_000761"
     CDS             37274..37933
                     /gene="yegL"
                     /locus_tag="ACHKUI_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6597020.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            37930..38691
                     /gene="pphC"
                     /locus_tag="ACHKUI_000762"
     CDS             37930..38691
                     /gene="pphC"
                     /locus_tag="ACHKUI_000762"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO6597021.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKLQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            38688..40628
                     /gene="yegI"
                     /locus_tag="ACHKUI_000763"
     CDS             38688..40628
                     /gene="yegI"
                     /locus_tag="ACHKUI_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6597022.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALVALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(40641..41993)
                     /gene="yegD"
                     /locus_tag="ACHKUI_000764"
     CDS             complement(40641..41993)
                     /gene="yegD"
                     /locus_tag="ACHKUI_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6597023.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLIVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPVAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            42127..42975
                     /gene="alkA"
                     /locus_tag="ACHKUI_000765"
     CDS             42127..42975
                     /gene="alkA"
                     /locus_tag="ACHKUI_000765"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6597024.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAVEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANSALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTLAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(43085..46402)
                     /gene="dgcE"
                     /locus_tag="ACHKUI_000766"
     CDS             complement(43085..46402)
                     /gene="dgcE"
                     /locus_tag="ACHKUI_000766"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6597025.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            46720..47361
                     /gene="udk"
                     /locus_tag="ACHKUI_000767"
     CDS             46720..47361
                     /gene="udk"
                     /locus_tag="ACHKUI_000767"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6597026.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MNSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            47453..48034
                     /gene="dcd"
                     /locus_tag="ACHKUI_000768"
     CDS             47453..48034
                     /gene="dcd"
                     /locus_tag="ACHKUI_000768"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6597027.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            48056..49909
                     /gene="asmA"
                     /locus_tag="ACHKUI_000769"
     CDS             48056..49909
                     /gene="asmA"
                     /locus_tag="ACHKUI_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6597028.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(49977..51560)
                     /gene="yegH"
                     /locus_tag="ACHKUI_000770"
     CDS             complement(49977..51560)
                     /gene="yegH"
                     /locus_tag="ACHKUI_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6597029.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(51761..51910)
                     /locus_tag="ACHKUI_000771"
     CDS             complement(51761..51910)
                     /locus_tag="ACHKUI_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597030.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            52219..53358
                     /gene="wza"
                     /locus_tag="ACHKUI_000772"
     CDS             52219..53358
                     /gene="wza"
                     /locus_tag="ACHKUI_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6597031.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53364..53807
                     /gene="wzb"
                     /locus_tag="ACHKUI_000773"
     CDS             53364..53807
                     /gene="wzb"
                     /locus_tag="ACHKUI_000773"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6597032.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            53810..55972
                     /gene="wzc"
                     /locus_tag="ACHKUI_000774"
     CDS             53810..55972
                     /gene="wzc"
                     /locus_tag="ACHKUI_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6597033.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            56101..56940
                     /gene="wcaA"
                     /locus_tag="ACHKUI_000775"
     CDS             56101..56940
                     /gene="wcaA"
                     /locus_tag="ACHKUI_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6597034.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTKLKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            56943..57431
                     /gene="wcaB"
                     /locus_tag="ACHKUI_000776"
     CDS             56943..57431
                     /gene="wcaB"
                     /locus_tag="ACHKUI_000776"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6597035.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            57428..58645
                     /gene="wcaC"
                     /locus_tag="ACHKUI_000777"
     CDS             57428..58645
                     /gene="wcaC"
                     /locus_tag="ACHKUI_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6597036.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQDKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            58620..59837
                     /gene="wcaD"
                     /locus_tag="ACHKUI_000778"
     CDS             58620..59837
                     /gene="wcaD"
                     /locus_tag="ACHKUI_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6597037.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMITLGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            59848..60594
                     /gene="wcaE"
                     /locus_tag="ACHKUI_000779"
     CDS             59848..60594
                     /gene="wcaE"
                     /locus_tag="ACHKUI_000779"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6597038.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYVFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            60610..61158
                     /gene="wcaF"
                     /locus_tag="ACHKUI_000780"
     CDS             60610..61158
                     /gene="wcaF"
                     /locus_tag="ACHKUI_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6597039.1"
                     /translation="MQDLSGFSVPKGFRGSNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            61185..62306
                     /gene="gmd"
                     /locus_tag="ACHKUI_000781"
     CDS             61185..62306
                     /gene="gmd"
                     /locus_tag="ACHKUI_000781"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6597040.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            62309..63274
                     /gene="fcl"
                     /locus_tag="ACHKUI_000782"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             62309..63274
                     /gene="fcl"
                     /locus_tag="ACHKUI_000782"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6597041.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTRPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            63277..63756
                     /gene="gmm"
                     /locus_tag="ACHKUI_000783"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             63277..63756
                     /gene="gmm"
                     /locus_tag="ACHKUI_000783"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6597042.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDTLLASDNVHANSRAYFLAEKRAGVPGL"
     gene            63753..64976
                     /gene="wcaI"
                     /locus_tag="ACHKUI_000784"
     CDS             63753..64976
                     /gene="wcaI"
                     /locus_tag="ACHKUI_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6597043.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNRAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            64979..66415
                     /gene="cpsB"
                     /locus_tag="ACHKUI_000785"
     CDS             64979..66415
                     /gene="cpsB"
                     /locus_tag="ACHKUI_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6597044.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGII
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            66608..67978
                     /gene="cpsG"
                     /locus_tag="ACHKUI_000786"
                     /gene_synonym="manB"
     CDS             66608..67978
                     /gene="cpsG"
                     /locus_tag="ACHKUI_000786"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6597045.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            68034..69428
                     /gene="wcaJ"
                     /locus_tag="ACHKUI_000787"
     CDS             68034..69428
                     /gene="wcaJ"
                     /locus_tag="ACHKUI_000787"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6597046.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            69430..70908
                     /gene="wzxC"
                     /locus_tag="ACHKUI_000788"
     CDS             69430..70908
                     /gene="wzxC"
                     /locus_tag="ACHKUI_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6597047.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DGIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSSALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
     gene            71473..72753
                     /gene="wcaK"
                     /locus_tag="ACHKUI_000789"
     CDS             71473..72753
                     /gene="wcaK"
                     /locus_tag="ACHKUI_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6597048.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            72750..73970
                     /gene="wcaL"
                     /locus_tag="ACHKUI_000790"
     CDS             72750..73970
                     /gene="wcaL"
                     /locus_tag="ACHKUI_000790"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6597049.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            73981..75375
                     /gene="wcaM"
                     /locus_tag="ACHKUI_000791"
     CDS             73981..75375
                     /gene="wcaM"
                     /locus_tag="ACHKUI_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6597050.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLICDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKPASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            75550..76443
                     /gene="galF"
                     /locus_tag="ACHKUI_000792"
     CDS             75550..76443
                     /gene="galF"
                     /locus_tag="ACHKUI_000792"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6597051.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            76816..77901
                     /gene="rfbB"
                     /locus_tag="ACHKUI_000793"
     CDS             76816..77901
                     /gene="rfbB"
                     /locus_tag="ACHKUI_000793"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6597052.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EISDSERYSFEHADICDAEAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRH"
     gene            77901..78800
                     /gene="rfbD"
                     /locus_tag="ACHKUI_000794"
     CDS             77901..78800
                     /gene="rfbD"
                     /locus_tag="ACHKUI_000794"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO6597053.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTVYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            78858..79736
                     /gene="rfbA"
                     /locus_tag="ACHKUI_000795"
     CDS             78858..79736
                     /gene="rfbA"
                     /locus_tag="ACHKUI_000795"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6597054.1"
                     /translation="MNTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLHYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGHLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAKPL
                     KKNAYGQYLLKMIKGY"
     gene            79741..>80267
                     /gene="rfbC"
                     /locus_tag="ACHKUI_000796"
     CDS             79741..>80267
                     /gene="rfbC"
                     /locus_tag="ACHKUI_000796"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO6597055.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHFQLPPFEQAKLVRCIVGEVFDVAVDIRPNSETFGSWVGVT
                     LSSENKRQLWIPEGFAHGFLTLSDIAEFVYKTNNYYSLNHERGVIWNDEEINIAWPSQ
                     SEKILSQKDINLPSF"
BASE COUNT        20002 a        19755 c        21209 g        19301 t
ORIGIN      
        1 atgtgcttaa gtatttgttg tgtttatatt gcaatagtta atttgcttta aatcaataaa
       61 acaatgtgtt tacattgcgg caactttttt ggtgtgagaa atttgtttcc aagagataca
      121 gactcttaac aaccaaaaat agattttatt aaggagcata ttatgaaacg ttcaattatt
      181 gctgccgctg ttttttcttc tttttttatg agcgctgggg tatttgccgc agacgttgat
      241 accggaacat taaccattaa agggaatatt gcagaatctc cgtgtaaatt cgaagcaggt
      301 ggcgattcag taagtattaa tatgccaact gtaccaacta cggtctttga aggtaaagct
      361 aaatactcga cctatgatgg tgcagtcggt gtaaccagca gcatgttaaa aattagctgt
      421 ccgaaagaag ttgctggtgt aaaactctcg ttgattacca acgataaaat aaccggaaac
      481 gataaggcga tagccagtag caacgatacc gtgggttatt atctctattt aggtgataac
      541 agcgatgtcc tggatgtttc tgcacctttc aacatcgaga gttataaaac agcggacggt
      601 caatatgcca ttccgtttaa agcaaaatac ctgaaactga cagataactc agtgaattca
      661 ggtgatgtgt tatcttccct gattatgcgt gtggcgcagg attaataaat aaaattgcaa
      721 gattcttcag gtaattaaca tggccgctat ctccatggcg gccttttaat ttaagaggca
      781 ttaagatgaa aggattatta gctttactca tttcttctat ggtccttcct gcacatgccg
      841 gtattgttat ctacgggacg cgtattattt acccggcaga gcacaaagaa gtgatggtgc
      901 agttgatgaa ccagggaaac cgttcttcgc tgatgcaggc gtggattgat gatggcgata
      961 cgtcattacc accagaaaaa attcaggttc ctttcatgtt aacgccacca gtggcaaaaa
     1021 taggggcaaa ttccgggcag caagtaaaaa ttaaaattat gccgaataaa ctacccacta
     1081 ataaagagag tattttttat ctgaatgttc tggatattcc accaaatagt ccagaacaag
     1141 aaggtaagaa tgcactgaag tttgcgatgc aaaacagaat taagttgttt taccggccag
     1201 cgggtattgc tccggtaaat aaagtgacct ttaaaaagtt actggtaaat cgcagtggca
     1261 atggattggt gataaaaaat gactcagcta attgggtgac gatttcggat gtcaaagcta
     1321 ataatgtcaa agtcaattat gaaactatta tgattgcccc cttagaaagt cagagtgtta
     1381 atgtcaaaag taataatgca aataactggc atctgaccat tatcgatgac catggcaatt
     1441 atattagtga caaaatttaa ttgcaggagc tgcctatgtt gagaatgacc ccacttgcat
     1501 cagcaatcgt agcgttattg atcggcattg aagcttatgc agctgaagaa acctttgata
     1561 cccatttcat gataggtgga atgaaagacc agcaggtttc aaatattcgt cttgaagata
     1621 gtcagccctt gccggggcag tatgacatcg atatttatgt caataaacaa tggcgtggga
     1681 aatatgagat cattgttaaa gacaacccgc aagaaacatg tttatcaagg gaaatgatca
     1741 agcggttagg cattaatacc gatagctttg ccagcggtaa gcaatgttta acatttaagc
     1801 aacttattca gggcgggagc tatacctggg atatcggagt ttttcgtctc gatttcagtg
     1861 tcccgcaggc ctgggtggaa gagctggaaa gtggctatgt tccaccagaa aactgggagc
     1921 ggggtattaa tgcgttttat acctcttatt atgtgagtca gtattacagc gattataaag
     1981 cgtccggtaa tagcaagagt acatatgtac gttttaacag cgggttaaat ttacaggagt
     2041 ggcaactgca ttctgatgcc agtttcagta aaacaaataa caatccaggg gtgtggaaaa
     2101 gcaataccct gtatctggaa cgtggatttg cccaacttct cggcacgctt cgtgtgggtg
     2161 atatgtacac atcaagcgat atttttgatt ctgttcgctt cagcggtgtg cggttgtttc
     2221 gtgatatgca gatgttgcct aactcgaaac aaaattttac gccacgggtg caggggattg
     2281 ctcagagtaa cgcgctggta actattgaac agaatggttt tgtggtttat cagaaagagg
     2341 ttcctcctgg cccgtttgcg attacagatt tgcagttggc cggtggtgga gcggatcttg
     2401 atgtcagcgt gaaagaggcg gacggttcgg taaccaccta tctggtgcct tatgcagcgg
     2461 taccaaatat gctgcaaccc ggcgtgtcga aatatgattt tgcggcgggt cgtagccata
     2521 ttgaaggggc gagcaaacaa agtgattttg tccaggcggg tcatcagtat ggttttaata
     2581 acttattaac gctgtatggt ggctcgatgg tcgcgaataa ttattacgcg ttcactttgg
     2641 ggactggctg gaatacacgc attggtgcca tttccgtcga tgccacgaag tcacatagta
     2701 aacaagacaa cggcgatgtg tttgacgggc aaagttatca aattgcctac aacaaatttg
     2761 tgagccaaac gtcgacacgt tttggtctgg cggcctggcg ttattcgtcg cgtgattatc
     2821 ggacatttaa tgatcacgta tgggcaaaca ataaagataa ttatcgccgt gatgaaaacg
     2881 atatctatga cattgccgat tattaccaga acgattttgg ccgtaaaaat agcttctctg
     2941 ccaatatgag tcagtcattg ccagaaggct ggggttctgt gtccttaagt acgttatggc
     3001 gagattactg ggggcgtagc ggcagcagta aggattatca gttgagttat tccaacaacc
     3061 tgcgaaggat aagctatacc ctcgcggcaa gccatgctta tgacgagaat catcatgaag
     3121 agaaacgttt taatattttt atatcgattc cctttgattg gggtgatgac gttacgacgc
     3181 ctcgtcggca aatatatatg tctaactcaa cgacgtttga tgatcagggg gttgcctcaa
     3241 ataatacggg attatcagga accgttggaa gccgggatca gtttaactat ggggtcaacc
     3301 tgagttatca gtatcaggga aatgaaacga cagctggggc gaatttaacc tggaacgcgc
     3361 cggttgcgac agtgaatggc agttatagtc agtcgagtgc ttatcgacag gctggagcca
     3421 gtgtttcagg gggcattgtc gcctggtcgg gtggcgttaa tctggcaaac cgtctttctg
     3481 aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc ttatgtcaat gggcaaaaat
     3541 atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg aatgacacct tatcgggaaa
     3601 attacctgat gttggatgtg tcacaaagcg atagcgaagc agaattacgt ggcaaccgta
     3661 aaattgccgc cccttatcgc ggcgcggttg tactggttaa ttttgatacc gatcagcgca
     3721 agccatggtt tataaaagcg ttaagagcgg atgggcaacc attaacgttt ggttatgaag
     3781 tcaatgatat ccatggtcat aatattggtg ttgtcggcca gggaagccag ttatttattc
     3841 gcaccaatga agtaccgcca tcggttaatg tggcaattga taagcaacaa gggctttcat
     3901 gcacaatcac cttcggtaaa gagattgatg aaagtagaaa ttatatttgc cagtaatagt
     3961 caggtggttc tatggaaatt cgcataatgc tatttatagt aatgatgatg gttatgcctg
     4021 tgagctatgc ggcatgttat agtgagttat ctgttcagca caatttggtt gttcaggggg
     4081 attttgcact tactcaaaca caaatggcga catatgagca taattttaat gattcgtcat
     4141 gcgtgagtac aaatactatc acacctatga gcccgtcgga tattattgtt ggactttata
     4201 acgataccat taaattaaat ttacattttg aatggaccaa taaaaacaac attacgttgt
     4261 caaataatca gaccagtttc accagtggtt attcggtgac ggtgacacct gcggccagta
     4321 atgcaaaagt gaatgtttct gcggggggcg gtggttcagt gatgattaat ggagttgcga
     4381 cattatccag tgcttcatca tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt
     4441 tattaggtgg taagtcatgg gatgcatgtg taaatagcta cagaaatgca ttggcacaaa
     4501 atgcaggtgt ctattccttt aatctgacat tgtcatacaa cccgataacc acaacctgca
     4561 aaccggacga tttattaatt acgttagaga gtattcccgt ttcacaatta ccagccacag
     4621 gtaacaaaac aacaataaat agtaaaaaag gggatattat tctgcgttgt aaaaatttat
     4681 taggtcaaca aaatcaaaca tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt
     4741 taaccaacag caacacgatc ctgaaaggtg cggaagataa tggcgtagga tttattcttg
     4801 aaagtaatgg tacgccagtc acacttttaa atatcactaa cagcagtaaa ggatatacaa
     4861 atttaaaaga aattgcggca aagtcaaaac ttacagatac aacggtttca attccgataa
     4921 cggccagtta ctacgtctac gatacaaaca aagttaaatc tggcgcactg gaggcaaccg
     4981 cattaatcaa cgtgaaatac gactaataac aagcaagacg agcaagtggc taataataaa
     5041 aatattaagg tgtctgcatt cccccctctc tccgaggggg ctttttttta gcgatgataa
     5101 aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac
     5161 gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt acgtaactgc
     5221 cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtgtc acctggtttg
     5281 aaatgcttaa agtcagcaat gaaactgctt aactcatact tagaaattcc cgaggtattg
     5341 gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcgcaccc
     5401 agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc
     5461 gcatatcttt cctgccatac aatataactg acaagcgata ttttgttagt gatcccatga
     5521 tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag cagagagtca
     5581 gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag cctaaaggat
     5641 tgagttatcg cattatgccc atgaagccat gtacgcccat atacacacca actaagccaa
     5701 tcaacagact ggaaaaatag ggagcgcgtt tagcgagagt gttaaatccg ctccagcgtt
     5761 ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc gttaacgcca
     5821 ggccgaggct gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc
     5881 aaatcaacag cacggtaatt gctgccgggc aggggataag gccaccggtt aagccaaata
     5941 gcaaaatttg ccagttggtg acctctctac catcaaagcg tcgtttaatg tcattggcat
     6001 gggctcgtgc atgggcatcc tgatactcgc catgttcgtg atggtgatga tgtccatggt
     6061 cgtggtgatc ttcgtgatcg tggtgatgat gctcatgatc atgctcgtgc atattctcca
     6121 gccagttgcg ttcgccgcgc caggtacgcc agaacatcca gaacgcggtg ctaataatga
     6181 tcactgcgga aatcaactgg agccacggtt ctgctgattg agcagtaaag cgtttgctga
     6241 tcaccatccc gccaaaggca attaaccaga ccactgcggt atgcgaaatg gttgctgcca
     6301 gtccgagcat caccgcctgt ttaatggtgc ctttgatggc gatgataaac gccgccatca
     6361 tcgtttttga gtgccccggt tccaggccat gaagcgcacc aagtaagatg gcgctgggga
     6421 tgaagaacca ggcgtttcct tgctgaagaa gtgttgtaaa ttcggtcatg ataataattc
     6481 ttagtattaa ttcggcaatc tgattctact cccccccagt acctgatact accccccagt
     6541 agattaatag tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac
     6601 aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc
     6661 gctcaagaaa atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc
     6721 tatccgtggc gcggtgaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca
     6781 catcgttcac cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt
     6841 gctggattca tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt
     6901 ttacaacgcg tgcgctggac attaccatcc tcctttgcga tttatcatcg caaccaaacg
     6961 actcggggtg cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat
     7021 gccagcgtag ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca
     7081 aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac acctttttca
     7141 ccaacattcc cctcttgtgc actgtatgac caacgatgtg gtgcaaacct ttactgccaa
     7201 taccttgctg gcgctcggtg catcgccagc gatggttatc gaaaccgaag aagccagtca
     7261 gtttgcggct atcgccagtg cgttgctgat taacgttggc acactgacgc agccacgagc
     7321 gcaagcgatg agtgctgccg ttgagcaagc aacacgatca caaacgccct ggacgcttga
     7381 tccggttgcg gttggtgcgc tcgattatcg ccgccgtttt tgtgtggaac ttctgtccca
     7441 taagccaacc gccatacgtg gtaatgcttc ggaaatcatg gcattagctg gcgttgctaa
     7501 tggtggacgg ggagtggata ccactgatgc cgcagctaac gcgatacccg ctgcacaaac
     7561 actggcacgg gaaactggcg caatcgtcgt ggtcactggc gaggtggatt atgttaccga
     7621 tggacatcgt atcgttggca ttcacggtgg cgatccgcta atgaccaaag tggtaggaac
     7681 tggctgtgca ttatcggcgg ttgtcgctgc ctgttgtgcg ctaccaggcg atacgctgga
     7741 aaatgtcgca tctgcctgtc actggatgaa acaagccgga gaacgcgcag tcgccagaag
     7801 cgaggggcca ggtagttttg ttccacattt ccttgatgcg ctctggcaat tgacgcagga
     7861 ggtgcaggca tgaaacgaat taacgctctg acgattgccg gtactgatcc gagtggtggt
     7921 gcggggattc aggccgatct taaaaccttc tcggcacttg gcgcttatgg ttgctcagtt
     7981 attactgcac tagtggcgca aaatacccgt ggcgtacagt cggtgtatcg cattgagcct
     8041 gattttgtcg ccgcccagct cgattcggtg ttcagcgatg tgcgaatcga caccactaaa
     8101 atcggtatgt tggcggaaac cgatattgtt gaagcggtgg cagaacggtt gcaacgttat
     8161 cagatccaaa acgtggtact cgacaccgtt atgctggcaa aaagcggcga cccgctgctt
     8221 tcaccttcgg cagttgccac gctacgcagt cgattattgc cacaggtttc attaataaca
     8281 ccaaacttgc ccgaagcagc cgccttgctc gacgcgccac acgcgcgcac cgaacaggaa
     8341 atgctggaac aagggcgatc gctgttggcg atgggctgtg gcgcagtgct aatgaaaggt
     8401 ggtcatctgg atgatgagca aagcccggac tggctgttta cccgcgaggg tgaacaacgg
     8461 tttaccgcac agcgcattat gaccaaaaac actcacggca ctggttgtac gctctctgcg
     8521 gcgttggctg cactacgccc gcgccataca aactgggctg acaccgtaca ggaggcaaaa
     8581 agctggcttt catcggcatt agcccaggcc gacacgctgg aagttggtca cggtattggt
     8641 ccggttcacc acttccacgc ctggtggtga gcaccgtact atagctgctt tggttattgt
     8701 cagcctttgg tgctgacatt cagggatgaa accaatgcag ttgagaatca ccagtcgaaa
     8761 aaaattcacc gttttattat gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg
     8821 tcagacggtg aattttttct actcgacagc aattcagatt aaagactaca tccacttcta
     8881 cggttatcgt ccggtcaaat cttttgctat tcgcattcct gccagttaca ccattcacgg
     8941 aatagatgtt tcgcgctggc aggagcggat caactggcag cgggtggcaa aaatgcgcga
     9001 taacggtatt cgcttacaat ttgcctttat taaggcgacg gaaggcgaaa agctggtgga
     9061 cccctatttt tcgcgtaact ggcaactaag ccgcgaaaat ggcctgctgc gcggggcgta
     9121 tcattatttt tccccgtcgg tatctgcttc agttcaggcg agattatttc tgcaaacggt
     9181 ggatttctca caaggcgatt tccctgccgt gctggacgta gaagaacggg gaaaattatc
     9241 ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa atggtcgaaa aacgtacggg
     9301 taaaaagcca attatttact caggagccgt tttttatcac accaatctgg cgggctattt
     9361 caatgaatat ccgtggtggg tggctcacta ttatcaacgt cgcccggaca atgacggcat
     9421 ggcctggcgc ttctggcagc attccgaccg tggacaggtc gatggcatta atggtccggt
     9481 ggattttaat gtgtttaatg gcacggtgga ggagttgcag ggattcgttg atgggattaa
     9541 agaaacgcct taaatatgaa gcagtgatat actcccacca gattccccgg caggaaaaag
     9601 acaaatggaa caagcgcata cccagctgat tgcccaactg aacgagcgta ttttagcggc
     9661 ggataacacg ccgctttata ttaagtttgc cgaaacggta aaaaatgccg tgcgcagcgg
     9721 ggtgctggag catggcaata ttttgcccgg tgagcgtgat ttaagtcagt taaccggcgt
     9781 ttcgcgcatt acggtgcgca aggcgatgca gtcgctggaa gaagagggcg tggtgacgcg
     9841 ttcgcgtggt tacggcacgc agatcaacaa catcttcgaa tactcgctga aagaagcgcg
     9901 ggggttttct cagcaggtgg tattgcgcgg gaaaaagccc gatacgttat gggttaacaa
     9961 gcgtgtcgtg aaatgtcccg aagaagtcgc gcagcagctg gcggtcgaag cgggaagtga
    10021 tgtctttttg cttaagcgta ttcgctatgt cgatgaagag gcggtatcga ttgaggaatc
    10081 gtgggttccg gcacatttaa ttcatgatgt tgatgccatc gggatttctc tttatgacta
    10141 cttccgcagc cagcacattt acccacaacg tacgcgttcc cgcgttagcg cccggatgcc
    10201 ggatgccgag tttcagtcac atattcagtt agatagcaaa ataccggtgc tggtgatcaa
    10261 gcaagtggcg cttgaccaac agcaacggcc tattgagtac agcatcagcc actgtcgcag
    10321 cgatctatac gtttttgtgt gcgaggagta gttcctcgcg cgttggcgca caatcaccgc
    10381 cccgatgccc gacaacccac gacgccactg cattgcccag taatacggca tccgccagtg
    10441 gcagaccaga ggccagcccg gcaagtacgc caccggcatg actgtcgccc gccccaatgg
    10501 tgtctacaac ttgcgttgga aatgccggaa tgcagccaga agcgtcgttg ctgaaatacc
    10561 atgcgccctc tttatcgaga cgaacgatca acggcgcggc aaatttctcc tgccattgct
    10621 tgcctagtgt tgttatctct gcggatagag caaaacgttc ggcggcaatc tcagcctctt
    10681 gacgattgag tgacactaaa ggtcgacagg ccatgatccg cgccagtaat gcatccggga
    10741 tatcgccaat acgtgggcca aaatcgataa atggcgtcac gtcttgcagc tcttccagcc
    10801 attccactaa caattcgccg cagggcgagg ccagttgata accggaaaaa tagagcaggc
    10861 tgccaggcgc aacggttaat cgcgccagcc actggcgatt ccactgattt tccacgccgc
    10921 tgaacgacat aaaagtgcgt tcgccatccg gctcaaccag cgccagacac cagccgttat
    10981 caccttcggc gttatcgatc aggctgatta agtcctcttt tgccatgcgg ttgcgaataa
    11041 tctccgccca caccccctga ccgagcggca aggcattacc cgcttcgatg ccgaggcgct
    11101 ttaatgccac ggcaatattc agggcgcagc cgccaacgtt aacgctctgc tgtttcagtt
    11161 cgatatcgca cccacgccag gggagggcat aagcgtcggc gatcacatca atgaccgccg
    11221 cgccaatcac cataactggc tgacgcgagg ttaattcagg cagcagcgtg tgtaatcgag
    11281 cgccgctcat atcgcctccc gttgttggcg atacttcgcc agcgccgtgg catagcggtt
    11341 gaaatcaagc tgatttactg tatccagttc cgcctttaac gcaggatcga tagtattaac
    11401 gccatgcaac gcgccgcaaa ttgccgtcgc catagcaccg atggtgtctg tgtcgccgcc
    11461 aaggttagcg cacaggacgg cgcaacggtt cgggtcggtt tgtgccagtt caaccagcgc
    11521 aatggcgcac ggaacggact caatagtact ggtacctgcg ccaacgacct ggtaaagctg
    11581 ttcgctggcg gattcggtgc cgtcagcatt gcgcacaatt ttcagcgcaa tctccagacg
    11641 tgcagccagt gaggcgctga aggtagtgat gcgtttttgt tgagcatgtc gcgcaattga
    11701 aggcagtgaa tcaacaattg ctgaccagct ttctccgtca atggcacgag aaatcgccca
    11761 tgcgatgact accgcccccg caaccgccag atcggattta tgtgtcgggc tggaggctag
    11821 cgccacatca tcaataaagg aatcaacatc acgcgccgga agcaaacaac ctaatggcga
    11881 gatgcgcatc gctgcgccgt tggtcacgcc gttattttcc agttcagcaa cgggtttacc
    11941 gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct agtacgtttt tgttaaaggc
    12001 gtcgaaacgc agcgcccagt cgagaatatt acgcccaatc agatccggat cgatcttgcc
    12061 ttcacgttcc agtaacgcat ccgccagaca cagcgccatc gaggtatcgt cggtgaattc
    12121 ggcgcggtta aaataacagg ctgcgttatt ctcctttggc ccaggcagaa aacggtcaat
    12181 ccagccaaag tgtgctttga cgcgactgcg cggccaaagc tcggagggca tccccatcgc
    12241 atcccctaac gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc
    12301 ccttgtgtca acgcaatatc gcgatcatcg accttgatag ccgtaatttc gttgtcggat
    12361 tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc
    12421 cacatcccgg cccagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt
    12481 tccatcatca caccgccaag acgatagccg agcaaactgc cgaagccctg gcagcagagc
    12541 gtgatcagcc cctgcgcggc ggtacgcata tgcacgggag cttttttatc gacatagatg
    12601 taagcggtaa cgtagtaaaa atcgtaactt acgccgtgca gcaaaatacc gaggaacagt
    12661 aacgcgtagg tgaaatattc atccgcacta ccgtaaataa agaagccata gcggatcgca
    12721 gcggtgacca gaccaagcaa caataccttt ttgataccaa agcgtttagt gaaaaacggc
    12781 aatgccagca taaagaagat ttcagagaac tggccgagcg tcatccagcc agtggcgttt
    12841 ttcatgccaa cttcggtcag gtaaccgttg gcaaagatgt aatagaacgc cagtggcatc
    12901 gcaaacagga atgaacagaa gaaaaagacg aggaagttct tatcgcgcag caggatcagc
    12961 gcatccaggc cgagcatgac tttaatatcc attttgccgg tgctttttgg tggcgtgtcg
    13021 ggcaggaaaa atgcaaacac accgagcaga gcagaacttc cggcggtaat cagtagcggg
    13081 atgttcgtcg gtgagatatc ggcatacccc agcatttgcg gcaagaatcc acatgccaga
    13141 ccagaggcaa tccagccgat agtgcccatc acacgaatgc gcgggaaatc acgctccaca
    13201 tccggcacgt tggcaaaagc gatactgtta gtcagcgcaa tggtcggcat ataggttagc
    13261 gagtaggcca gcagtaacgg gaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac
    13321 atcagcaccg caccagcgaa catcaatacc gccagcactt tctgcgccga gaaaaagcgg
    13381 tcagtgatgg agccaaccag aatcggcgac aggatcgccg caatggcggt acaggcatac
    13441 gaccagccaa tttctccggc actaaaaccg cttttactta accacaacca caatggcaca
    13501 aaccacgcgc cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt
    13561 ttcatctttt aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacttag
    13621 taataccttt taaatacctt tgcggtgaaa acttgaccgc ggtaacattt tttctgatga
    13681 atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg
    13741 accaggatta tgattgcccg caaaaggcgg gccaggacaa tccatagccg atatccaatc
    13801 ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac
    13861 aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat
    13921 gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg
    13981 cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat tctgcccgtt
    14041 gaccagggcg ttgagcactc tgccggagct tcatttgccg ctaacccgct ctactttgac
    14101 ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc gtcaacttac
    14161 ggcgtgctgg cgtcggtatc gcggcgttac gcgcatcgca ttccattcct cgtcaaactt
    14221 aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg
    14281 gagcaggcgt tcaatatggg cgcggtggcg gttggggcga ctatctattt tggctcggaa
    14341 gagtcacgtc gccagattga agaaatttct gcggcttttg aacgtgcgca cgagctgggc
    14401 atggtgacag tgctgtgggc ctatttgcgt aactccgcct ttaagaaaga tggcgttgat
    14461 taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca
    14521 gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt
    14581 tataccgacg atcgagtcta cagcaaactg accagtgaaa acccgattga cctggtgcgt
    14641 tatcagttag ctaactgcta tatgggccgg gccgggttga taaactccgg cggtgctgca
    14701 ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc
    14761 ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa
    14821 ctgattaacg ccgtgcagga cgtttatctc gatagcaaaa ttactatcgc ctgatgcgcc
    14881 tcatacgaac agcaccaccc cgtaggacgg aaaaggcgtt tacgttgcat ccggcataaa
    14941 aacacgcgca cttcgttacg gcttcccccg ggaggccgtt ttttttgcct gtcattctga
    15001 ttaattttca cattgtcgtt tttgtgatcg ttatctcgac atttgaaaac aaataatttc
    15061 attgtgtttt gaaatcgaaa acaaacgaca ggatatgaaa atgtacgtgg tatcgacaaa
    15121 gcagatgctg aacaacgcgc agcgcggcgg ttatgcggtt ccggcattca atattcacaa
    15181 tctcgaaacg atgcaagtgg tggtggaaac cgctgccagc atgcatgcgc cggtcatcat
    15241 cgccggaacg cccggcacat ttactcatgc cggtacagaa aatctgatgg cgctggtcag
    15301 cgcgatggcg aagcaatatc accatccact ggcgattcat ctcaaccatc acacgaaatt
    15361 tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca gtcatgattg acgcctcgca
    15421 tttgcctttt gcgcaaaata tttcacgggt caaagaggtg gtggattttt gccatcgctt
    15481 tgatgtcagc gtcgaagcgg agctggggca acttggcggc caggaagatg atgtgcaagt
    15541 caatgaagcc gatgcttttt ataccaaccc cgttcaggcg cgtgaatttg ccgaggcaac
    15601 cgggattgat tccctggcgg tcgccatcgg cacggctcac ggtatgtatg ccagagcacc
    15661 ggcgcttgat ttttctcgac tggagaacat tcgccagtgg gtgaatttac cgctggtgct
    15721 gcatggcgcg tcaggattat cgactaagga tattcagcaa accatcaaac tggggatatg
    15781 caaaatcaac gttgcaacgg agctgaaaaa tgccttctcg caggcgttaa aaaattacct
    15841 gaccgagcac cccgaagcga ctgatccccg tgattatttg cagtcggcta aatctgcaat
    15901 gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc gagggcaggg cataacgcac
    15961 ctgccattta acaaggaaaa aacatgaaaa cgttaattgc ccggcataaa gctggtgaac
    16021 atattggcat atgttcagtc tgttctgcac atccgttggt tatcgaagcg gcgctggcat
    16081 ttgatcgcaa cagcacgcgt aaagtgctga ttgaagcaac gtcaaaccag gtcaatcaat
    16141 ttggcggtta taccggaatg acaccggcag actttcgcga atttgttttt gcgattgccg
    16201 ataaagtcgg atttgctcgt gagcgtatta ttctcggcgg cgaccatctg ggaccaaact
    16261 gctggcagca agaaaatgcg gatgcggcga tggaaaaatc cgtcgagctg gtaaaggcat
    16321 atgttcgtgc cggcttcagt aaaattcatc ttgatgcgtc aatgtcctgc gctgacgatt
    16381 ccataccgtt agcgccagaa acggttgctg aacgtgccgc tgtgctttgt ctggcggcgg
    16441 aaagtgtggc gactgattgc cagcgtgagc aactgaacta tgtcattggc actgaagttc
    16501 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccag gttgaagatg
    16561 ccgccaatac tttacgcacg catcaaaagg cctttattgc ccgtggtctg gcagaggcgt
    16621 taacacgcgt gattgccatc gtggtgcagc cgggtgtgga atttgaccat agcaatatta
    16681 ttcattatca ggcgcaagaa gctcaggcgt tggcgcaatg gatagaaaaa accaaaatgg
    16741 tttatgaagc gcattccacc gattaccaga ctcagacggc ctatcgggaa ttagttcgcg
    16801 atcattttgc aattttgaaa gtcggtccgg cattaacctt tgctttacgt gaggcgatat
    16861 ttgcgctggc acaaattgag caggaactta tcgcacccga aaatcgtagc cgttgtctgg
    16921 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    16981 gttttaacga ttcattactg ggtattcgtt acagcctgtc ggatcgtatt cgttattact
    17041 ggccgcatag ccggattaaa aatagcgtcg aaacgatgat ggtgaatctg gaaggcgtgg
    17101 acatcccact gggcatgatt agtcagtatc ttccgaaaca atttgaacgc attcagtccg
    17161 gggaattatc agcaataccg catcagctta ttatggataa aatttatgat gttttgcgcg
    17221 cctatcgcta cggctgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    17281 gaatttcttt tgtcgatcgt agcgaagttt taacccatat cggtaatgag atgctcgcga
    17341 aaggtgtggt tcatgatacc tggccacagg cattaattac cagagaagca gaattcccta
    17401 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    17461 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    17521 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    17581 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    17641 taatcactct tcctgaaacc cagttaaagg aatacttcac aaagtatgtt ttagattcag
    17701 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaaa attattgtcg
    17761 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    17821 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    17881 tggatggcgt gcatttgata tgcaccactg ccagggtgga tcgtagtttt ggcaatattc
    17941 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    18001 ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc
    18061 tcggccctac ggtgatgctg ccgattgtca tcattatgtt ttctaaaata ttaggcatga
    18121 aggcaggcga ttgctttaaa gcaggtctgc atatcgggat tggctttgtt ggcattggcc
    18181 ttgtgattgg cttaatgctg gattccattg gcccggcggc gaaagcgatg gcggaaaatt
    18241 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcaccc atgacctggg
    18301 cgtcgcaaat tgcgctggtg gcgattccga ttgcgattct ggttaacgtg gcgatgttac
    18361 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    18421 ccggcgcgtt gctgcatctg gcaaccggtt catggatgat agggatggcg ggtgtggtaa
    18481 ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc cgaaatttct
    18541 ttgagctgga aggcattgct attccgcacg gtacgtcggc gtatatggga ccaattgctg
    18601 tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa tttagtgccg
    18661 acgatattca gcgcaaattt ggtccgtttg gcgagcctgt caccgtgggt tttgtgatgg
    18721 ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa
    18781 aaacggcggc ggttatgctg ctgatgccac gggtgattaa acccatcatg gatggtttaa
    18841 cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc
    18901 tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg gcaagcctga
    18961 tttttatccc gctcaccatt ttaattgctg tttgtgttcc gggtaatcag gtgctgccgt
    19021 ttggcgatct tgcaactatc ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa
    19081 atctgttccg caccttaatc tcgggtgtca tcattatgag catcaccctg tggatcgcga
    19141 cgcaaactat tggtttgcac acccaactgg cggctaatgc tggggcgtta aaagccgggg
    19201 ggatggtggc ttcaatggat cagggcggtt ctcccattac ctggttactg attcaggttt
    19261 tctccccgca aaatattccc ggtttcatta ttatcggcgc aatttatctg accggtattt
    19321 tcatgacctg gcgtagagcg cgtggtttta ttaaacaaga gaaagccgtt ctcgcagaat
    19381 aatttttacc tgagggggtg gtaatccccc ttaacaatca ggagttttta tgaaatcagt
    19441 ggtgaatgat actgatggta tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca
    19501 tcaggatgag gtgcgggtaa aaattgccag ctcgggctta tgtggttccg atttacccag
    19561 gatatttaaa aatggtgcac attattatcc aataacgtta ggccatgaat ttagcggcta
    19621 tattgatgcg gtgggatccg gtgttgatga tttacacccc ggcgatgcgg ttgcctgtgt
    19681 gccgttatta ccctgtttta cttgtccaga gtgtctgaaa gggttttatt cccagtgcgc
    19741 aaaatatgat tttattggct cacggcgtga tggtggattt gccgaatata ttgtcgttaa
    19801 gcgaaacaat gtctttgcac tacctgcgga tatgcctatt gaggatgggg cttttattga
    19861 accgattacc gttggcctgc atgcgttcca tttagcgcaa ggatgtgaga ataaaaacgt
    19921 tattattatt ggtgccggaa ccattggcct gctggcaatt cagtgcgctg tcgcgctggg
    19981 agcaaagagt gtgacggcta tcgacattag ctcagaaaaa ctggcactgg caaaatcttt
    20041 cggtgcgatg caaacattta acagccttga aatgagcgcg ccgcaaatgc agggcgtttt
    20101 acgcgagctg cgctttaatc agcttatcct cgaggcggct ggcgtcccgc aaaccgtcga
    20161 actggcggta gagattgccg ggcctcatgc ccagctggcg ctggtgggca cgttgcatca
    20221 ggatctgcat ttaacatcgg caacgtttgg caaaatattg cgtaaagagc tgacggttat
    20281 cggcagttgg atgaactact ccagcccttg gccggggcag gagtgggaaa cggcgagtcg
    20341 gttgctgaca gaacgtaagt taagcctgga gccattaatc gctcaccgtg gaagctttga
    20401 aagcttcgcc caggcggtgc gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct
    20461 cattccctga aacctcgggc cagtgagatg ctggcccggt attgtgcaaa acagatcatt
    20521 caccaatggt cccccttcgt ttacactagc cacaattgaa tatgggtaaa tgacgatgaa
    20581 ttcattcgag cgaaggaata agatcatcca attagtgaat gaacagggaa ccgtgcttgt
    20641 tcaggatctg gcgggagtat ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt
    20701 tctcgaacaa aaaggcgtgg ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg
    20761 taatagtgaa accgagaccc aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc
    20821 gccaaaaaac agaatagcgc aggcggcagt caaaatgatc cacgaaggga tgaccgttat
    20881 tctcgacagc ggaagtacga caatgcttat cgccgaagga ttaatgaccg ccaaaaatat
    20941 cacggtgatt accaacagtc tcccggcagc atttgccctt tccgaaaata aagacattac
    21001 tctggtcgtc tgcggtggca cggtccgcca taaaacgcgc tcgatgcatg gctccattgc
    21061 tgagcgttca ttgcaggata ttaatgccga tttaatgttt gtcggtgctg atggtattga
    21121 tgcggttaat ggtattacga cctttaatga aggctattcg attagcgggg cgatggtcac
    21181 agccgcaaat aaagtgattg ccgttctcga ttcatcgaaa tttaatcgtc gcggttttaa
    21241 tcaggtactg ccaatcgaaa aaattgacat tattattacc gatgatgctg tgtctgaggt
    21301 ggataaactg gcattgcaga agacacgggt aaaattaatt acggtgtagt attgttctgt
    21361 atgccggata aggtgtttac accgcatccg gcattacgga ttctccctgg ttcaacagag
    21421 aatagtttga ttaacgcaat aatggacaat ctggtggtaa tcgacaacgc aacgccgccg
    21481 gaagtatttc aatatggaag ttttgcccac tcaacggttc gccatcaaga ttaaaagtga
    21541 tttcgtgtgg ggcttgtata tcgaaccacg ccgaagcacc ttcgataata ttcgggttat
    21601 cttcgtcaga ttttaaggtt gatacgagag ccggaagaat ttcatcgccg gtgaaaatgc
    21661 gcagttgcag taagccgtcg ttaattaatg cgttcgggca caattgctga ccgccaccgg
    21721 cctgacgccc gttaccaata ccaatgacca gggcgtcacc ttgccagtga aagttttcac
    21781 cgcggatttc acaacggtcc ggttgcagag tatccatgcg cattaagcca tgaatgatgt
    21841 aagagacgcc acccagcgcg gcttttaatt tttccggcgt ttctgtggta atacgcgtcc
    21901 caaatccgcc tgtcgccata ttaataaaac aggtttgttt gttgacctgc gccatatcta
    21961 tcgcaatggc gttaccggca attgccagtt tcagcgcctt atccagtgcc tcaggaatcc
    22021 ctacactggt ggcaaaatca ttggcggttc ctaatggcag aattcccagc gcgggtatgt
    22081 catccccctc acactgaatc aacgccgtag aaacttcatt aatggtgcca tcgccaccac
    22141 cggcaatcac cgttgcgacg cccaacttcc gggcctcctc tacatatcgt gcggcatcgc
    22201 ctttctccca ggtgacccgc acatggatcg tcattccttc ctcacgcaac cgcataattg
    22261 cttcgcgcaa gggtagattg tcagtacttt tgccattaag aatcagtaag ctggcgggaa
    22321 attctgccat gatcgtatgt gcctttatga ttggtctgta gatagtgtag agcagaaaag
    22381 aaaaggtaga gtcagaacag gatgaaagtc ggaggataaa aagtcagttc aagtatggaa
    22441 gattcagtgg ttaccaccag gagcttgcaa tggggacagc gaatactaac ggcagccacc
    22501 gataaatttc aaaaaagagc atatacctaa tattcaacta aacagtggca tcttcaatat
    22561 actattaaaa atatattaac ccccccatgg ggttacccgg aagggcatcc gtgtatgtaa
    22621 ttcctactta tgtaggaaat gttgtacaga acatttatta taatcctatt caattataat
    22681 aatcatgcca ttattatatt taaacaccag agcgtgtcgt tggtatttaa tgggggaagg
    22741 taagatgaaa aagatagctg ctatatcatt aattagtgtt tttcttatgt ctgggtgtgc
    22801 tgtacataat gatgagacaa gtatcggtaa gtttggtctt gcatataaaa gtaatattca
    22861 gcgtaaactc gataaccaat actacaccga agccgaagct tctttagcca ggggtagaat
    22921 atctggtgca gaaaatatag taaaaaatga tgcagttcat ttctgtgtta ctcagggcaa
    22981 aaaaatgcag atagttaaat tgaagacaga aggtgcagga ttacatggcg tcgctcgtct
    23041 gacattcaaa tgtggagagt gagaatattt tttggtaagc gtcaaacatg cgcgttctgg
    23101 ctgtgcgtaa ccggaacctg tgcgagcacg atgccaataa gtgaaaggca tcgtgctatg
    23161 aagggagatt ctatcgatgt ggtcaatgga agacggttac caggatacgg cttatgcata
    23221 aaaaataagc ccgtgtaagg gagattacac aggctaagga ggtggttcct ggtacagcta
    23281 gcattttatg ggttatgttt ttcagcgaaa cggatgataa ccttaataaa tgcagctgta
    23341 tgtgatcggt ttctaagaat tttccatccg ggaaaaataa tcgaaattaa tcacttaccg
    23401 tgggggttac gcgtggtttc cccggagaaa ttacgcatca gcagcgcgta atttagctca
    23461 agatcctgcg ggaccgggat ccacacagta taaccatcgc ctggtgcgac cggcattgct
    23521 tcacctttgg cgttttccat atgctcaagg gtaaagttaa tgttgccttg cggcgtcatc
    23581 agctcaaggc tgtcgccaac ggagaattta tttttcaccg ctaccgccgc gaggtcgccc
    23641 ttgcgctcac cggtaaactc accaacaaac tgctggcggt cagaaactga ataaccgtat
    23701 tcgtagttct gataatcgtc gtgagtatga cgacgcagga aaccttcggt atagccacga
    23761 tgcgccagac cttccagagt ttccagcagg ctggtatcga atggttttcc cgcagctgcg
    23821 tcatcgatag ctttacggta aacctgcgcg gtgcgtgcgc aatagtagaa agatttggta
    23881 cgaccttcga ttttcagcga atgcacgccc attttggtca ggcgttcaac gtgggcgata
    23941 gcgcgcagat ctttcgagtt catgatgtaa gtgccgtgct catcttcaaa cgcagtcata
    24001 tactcgcccg gacgctgagc ttcttcgatc ataaacactt tgtcggttgg cgcgccgatg
    24061 cccagcgtcg gctcaacatt ttgcaccgga atcggctcgt acttgtgcac gatgttgcca
    24121 acgtcatctt ctttcccttc ctggacgttg tactcccagc ggcaggcgtt ggtgcaggtg
    24181 ccctggttcg ggtcgcgctt gttgatatag ccagagagca ggcagcgacc ggagtaggcc
    24241 atgcacagcg cgccgtgaac gaagatttcg atctccatat ccggcacctg attgcggatc
    24301 tcttcaatct cttccagtga cagttcgcga gagaggatca cgcgggtcag acccatttgc
    24361 tgccagaatt tcaccgtcgc ccagtttacg gcgttagcct gtaccgagag gtggattggc
    24421 atttccggga agtgctcacg caccagcata atcagccctg gatcggacat aatcagcgca
    24481 tccggcccca tttccaccac cggtttcagg tcacggataa aggttttcag cttggcgttg
    24541 tgcggtgcaa tgttgaccac gacataaaac tttttcccca gcgcgtgggc ttcattgatg
    24601 ccgagctgaa gattttcgtg gttgaattcg ttgttgcgta cacgcaggga gtaacgcggc
    24661 tggcccgcat aaacagcatc tgcgccataa gcgaaagcgt aacgcatatt tttcagcgtt
    24721 cccgccgggg aaaggagttc cggtttaaac ataattctct cgttctgatg acaggtcaga
    24781 gccgcttcac ctgatgaagc ggttagggga gtgcccccac tttaagggcg ggcattgtag
    24841 cgctgtgggg gtaactggta aagcgccgga cgccaacaac ctacgcccgg catcgtaatt
    24901 acgtattgtc tttcaccgtc tggcttgtgc catttacttt aacggaggct attccggttt
    24961 cacgagattg cgcggtggca tacatctggc tggagccgat aatttgatga ttggcggctt
    25021 tcagattgaa atagaatttg ccattgcttg ccgttttttt ctcatagcgt tcttcttgcg
    25081 ggctgttgct acgcaccgac gcgatgccct tttccgcaga ggcttttgag gtatacagct
    25141 cgctggtgag gatagtctca ccattgcccg cttttagcac aaaccggaac tgattatcac
    25201 tgctcttact gagttcaaac caaccagcca taatgactcc ttgattgaaa ttgcgtatcg
    25261 aaaacagcat ctaaaggtac tgtttgccaa ttaaagtatg agcgattttc tggtgcctgc
    25321 cagggcggag agataatgtc agtttgctga ccatgcgtgg gataatgttg ccggatctgg
    25381 tctgaaggcc atctccggca ggtaaaaatg ttaaacgatg cggcaggcgt cggcttccca
    25441 gcggtaaccg acgccgtaaa cggcgcggat aaatgactgt tcggcgtcga gagattccag
    25501 cttgcggcgc aggtttttaa tgtggctgtc gatggtacgg tcggttacca cgcggtagtc
    25561 gtcataaaga tggttcagca actgctcgcg ggagaacact tttcctggtt catgagagag
    25621 cgttttcagc agacgaaatt ctgcaggcgt caggtcaagc attttaccgc gccaggaagc
    25681 ctgaaaacga ccttcgtcga taatcaacgg gctttcagca tcctgttgct gcaactcgcg
    25741 ctgcggtttg caacggcgca aaatggtttt gacgcgcgct accacttccc gtgggctgta
    25801 cggcttgcag atataatcat ctgcgccaat ctccagtccg agcaggcagt cgatctcttc
    25861 gatttttgct gtcaccatca cgatcggaat gtcagaaaaa cgacgaatct cccgacacag
    25921 cgtcaggcca tcggtgccag ggagcatcag atccaacagt atcagatccg gtggtgtctg
    25981 gcgcacatat ggcagtacct gatcgccgtg gctaatcaac gttggcgcat agctcgcagc
    26041 acgcagataa tcaatgagca actgccccag cttcggttca tcttccacga tcaaaatacg
    26101 cggtgtgttt tcgtcgattg gtaactcggt catacttctc tctgtaaatc ccgttccagc
    26161 ggtaactcta ctgtaatgct aagcccgcca aaaggcgaat gggcggcaat aatgcggccg
    26221 ttatgtgctt caacaatgtt caggcaaatc gccagcccca gcccggaacc accgctggcg
    26281 cggttgcggg aaccttcggt gcgataaaaa cgttcaaaca atttttgtag ctgatcgtca
    26341 ctgacacccg gcgcactgtc ggcaaaggtc aggcgcaccg ttttgtcgtg ctgctcggca
    26401 gagattttca ggctgccgcc gctgtcagtg tagcgcaggc tattttccag taagttattg
    26461 aacaactgca ttaaacgatc gcgatcgcca aatacggtaa tactgtctgg cagggaaaat
    26521 tgcaatttca ggccacggct ggcgaatcgt tcgcgaaatg cgcctcccgc cacttccagc
    26581 agtgggatca aatctaccgg tgctttttga taggcgagag cgccttcatc agacatagac
    26641 aattgatgga gatcgtcaac cagttttgtc agtgtaccga cttctgcctg taaagaagcc
    26701 agcgtctccg gcgtgaattt acgcacacca tcctgaatgg cttccagttc gccgcgcagt
    26761 actgctaaag gcgtgcgcag ttcatgagaa atatcggcca taaaatcgcg gcgcatctgc
    26821 tggtttttct ccagtgtgct ggcgagttga ttgaaatcct ctgccagtct gcccaactca
    26881 tcttcgctgg tgggggctac gcgagtagtg aaatcacccg ctgccagttt gtgcgtgcca
    26941 tccaccagtc gttttaccgg agccagcaaa ccgcgcgcca gtgggaatgt cgccagcgcc
    27001 gctaataagg tagataaagc cacaatcagc caactggttt gccgctgttg tctgtcaaaa
    27061 ttgatatcag tattacgggt cagtcgttca acaggggagg cgatcaccgc gccaacttcc
    27121 gcaccattga ccataatggg ccgccgtgtg ccgtcgggtg gaaccggtgc tcgcgggcca
    27181 accagcactt tgttgttttg atcaaccacc caaaattgtg tgcgccagcc gtgtggcggc
    27241 ataccggggc cgggcttatc ttcgttgtta tcatgctcca gtgaacgtag gatctgaaat
    27301 acaaagcgat cgttattacg caggaagcgc cagttcccgt gctgggcgta ctgctcacca
    27361 agcgcatcgc cgagcatttg cagccgttgt tcattaccat gcttgatgta atcgataaag
    27421 ccgcgctcaa aactgatacg taccgcccag tgcatcgtga tcaataagac aatgcaggta
    27481 gcgaaaatcg ccagaaacag tttgccggta ataccgggtc gccagaactt cattgcacgc
    27541 tccttttccg ccgtgaaata gcgacatttt gatgcgtatc gctcggcact ctggcaaaga
    27601 caaacgccgg aagggcgatg ataaacgcca tgctaagcca ggtgtacata aagacggttt
    27661 gtgtggtgcc gctgtcgacg ctgacatgct gcgaaccaaa aagtcccagc aacagcccgg
    27721 cgatggtgac gccaatactc atcgacaatt gcataatcat cgacagcaga ctgttgccgc
    27781 tgctcgccag gttgtccggg agatctttca gcgtcagggt gttcatggag gagaaacgtg
    27841 tcgagttgac cattccttgt aaaaacagga cgaacggcaa aacgtagtac cagcccagca
    27901 gagcggtagt cataaacaac agggtgacca gcgacaggcc cagcgtggtc gccaccagta
    27961 cccgacgata accaaagcga ttcactacct gtaccacaat tctcttcatt cccatgctgc
    28021 cgagtaccat cgggatcatc atcagtccgg catgaaacgg cgagaaaccg aggccaattt
    28081 gcaggaaaac cggtgtcata aagggcaaca tgccactgcc aatacgtccg gcaaagctcc
    28141 ccgccaggcc cagcgaaaag gtacgagtgc ggaacagttt cagactgaac agggcgcggt
    28201 tgttattttg ggcgtgcagc agataaagca ccagtgctac cacgccaact gcgaccaggc
    28261 cagcaatcgc cagcggcgag aaacctgtac ctttactgcc gtccagcgcc agggttaata
    28321 ccgccatgcc aaccgccagc aacagaaagc cggagagatc aaagcgccgc gtctgcatgg
    28381 tgtagttcgg cattaacatc agtgtggcga tcgccccaat aatccccacc ggaatgttga
    28441 tcaaaaagat ccagtgccac gatgcgtact ccaccagcag accgccgagc gccgggccaa
    28501 gcagcggccc gacctgaccg ggcagcgtga caaaggtcat cgccgccata tattgctcgc
    28561 gcggtacggt tttcatcacc gtcaacctac cgaccggcac catcatcgca ccgccaacgc
    28621 cctgtaacgc gcgtgccagc aacagttcgt tcagcgtgcc ggaaagtgcg caaaacagtg
    28681 aaccgagagt aaacagcacg atggcggtaa agaaaatatt gcgcacgccg actttgtccg
    28741 ccagccagcc gctggcgggc agcatcaccg ccacggtcag cacataagag acaatgacca
    28801 tgtgcatatg caacggactt tccccgaggc tttgcgccat tgagggaagg gcggtgttta
    28861 cgatggtggt gtccagcgac tgcataaaga agccgaaagc cacaatccac aattgccagc
    28921 gggtgctgtc gggaagatct gtcatttact cggttaccgc ttgtttaggt ttacgcgaaa
    28981 aacgcagccg cagacggtcg aaaaagagat acaccaccgg cgtggtatac agcgtcagaa
    29041 gctggctcat gaccagaccg ccgacaatgg tgatccccaa tggttgccgc aactccgagc
    29101 cgtcaccgcc cgacaatacc agcggcagcg caccaaacag cgccgccagg gtagtcatca
    29161 taatcgggcg aaaacgcagc agacaggcct ggaaaatagc ttcctgcggc gtcaggttac
    29221 cgtgccgttg ggcttcaagc gcaaaatcga ccatcataat ggcgtttttc ttcacgatgc
    29281 cgattaataa catgatccct atcagcgcaa ttaggctgaa cggggcattg aacagctcca
    29341 gcgccaacag cgctccgacg cccgccgagg gtagggtgga gagaatcgtc agtggatgca
    29401 cataactctc gtaaaggata cccagtacga tatataccgt ggcgatggcg gcgataatca
    29461 ggatcacctg tgagttcatc gtctcctgga acacttgcgc tgttccggca aaactgccgc
    29521 gcaccgtcga aggcacacca agctgggtca ttgcgcgatc gatcgccgca ctggcgtccg
    29581 agagcgattt cccggtcggc aggttgaacg aaatggtcga ggccgccgat aatccctgat
    29641 gattcaccga cagtggagcg ttcgccggtt gccatttagc gaaatacgac agcgggatcg
    29701 cttttccttc gttattgata acgaacattt tttccagtgc actgatgtcc tgggtatagc
    29761 gcggatcaac ttccatcacc actttgtact ggttcatcgg ctggtaaatg gtcgagattt
    29821 gccgctgacc gaaggcgtta tttaacaggc tgttggcggc ttgcacgtca attcccaatc
    29881 gcgccatagt gtcgcgatcg taaataaggt tcatctccgc gccattatct tcctgatcgg
    29941 agttaacatc agccagctct ggcaatgtcg ccagtttttt gcggattttt ggctcccatt
    30001 cgcgcagtgc cgccagatcg tcggacaata atgtgtactg gtagctggcg ttagcctgac
    30061 gtccgccaac gcgaatatcc tgtaccgcca tcaggaacag attcgctccc ggttctttcg
    30121 ccagtttttt acgcaagcga tcgataatct gttgggccgt ttcgctccgc tcgccgcgtg
    30181 gtttaagggt gataaacatc attccactgt tcacccgtga accgccggta aagccggtga
    30241 cattatccac cgccggatcg tcacggataa ttttcatgaa gtcctgcaac ttaccgcgca
    30301 tcgcctgaaa cgaaatactc tggtcagcct gtatcccgcc catcagtacg ccggtgtcct
    30361 gctccgggaa gaaggttttc gggatcgaga tatacagcca gatattcagc gcaatggtgc
    30421 caagcagcac cgcgcccacc agacgggtat gattgagcac ccattttagt gacttgccgt
    30481 agccttgttg cagagcaacc aacatgcgac caaagccacg cagtcgcttt tgctcgcgcg
    30541 gcttgctggc ttttagcatc cagccgcaca tcatcggcgt taacgtcagg gaaaccagca
    30601 gcgaaatacc aatggcgaca gaaagcgtca cggcaaactc gcgtaacagg cgacccggca
    30661 atccgcccat caacagcagc ggcaggaaca ccgccaccag tgacagactc atcgacagca
    30721 ccgtaaagcc gacttcgcga gtaccttgca gcgcggcctg tagcggtttc atccccgctt
    30781 ccagatgacg ggcaatgttt tccagcacca cgatggcgtc atccaccaca aagccagtgg
    30841 cgatggtgag cgccattaac gaaagattat tgagactgaa accacacagg tacatcgccg
    30901 caaacgtacc aatcagcgaa accggcaccg caacggcggg aataatggtg gcgcgacccg
    30961 aacgcaggaa caaaaacacc accagaatca ccagcgccac cgagataatc agcgtctgct
    31021 caacctcttc cagcgacgcg cggatagtag gggagcgatc ctgggcaatt tgcagatcaa
    31081 ttgctgccgg gatggtggat tgtaattccg gcagacgggc gcggatgctg tctaccgtct
    31141 ggataatatt ggcttccggc agcttgcgga tcatcagcaa aatggccggt ttggcgttgg
    31201 tcatcccggc gttacgtaca tcttgtacag agtcggtcac cgtcgccaca tcgcccagac
    31261 gaaccgcgcc gcctttgttg taatgaataa tcagcggctg atattcagcg gcagttttca
    31321 gttcgtcatt ggtctggatc tgccagcggt gggtgccatc ttccagtgcg ccctgcggtt
    31381 tacgcacgtt ggcattgctg atggcagtgc gtacgtcgtc cagcgagacg ccctgattaa
    31441 acagcgcctg cggattcagc ccgacgcgta cggcgggcag cgagctgccg ccaacatcga
    31501 catcaccgac accgtcgatt tgcgaaatcg tcggagccag ctgtgtcgag gcgaaatcgt
    31561 acagttcacc ctgcgaataa gtatcggatg tcagcgtgag gatcataatt ggcgcatccg
    31621 atgggttcgc tttgcgatag gtcggacggc tgggcatccc gctgggtagc agactttgcg
    31681 cggcgttgat cgccgcctgc acatcgcgcg ctgcgccgtt gatatcccgg tcaaaatcaa
    31741 actgcaaaat aatgcgcgtg ctgccgagcg aactgctgga ggtcatttcg ctgactccgg
    31801 caatgcgccc aagtgagcgc tccagcggcg tggcaacgga agacgccatt gtttctggtg
    31861 acgcaccggg cagcgaggcg ctgaccatga tcaccggaaa atcgacctgc ggcagcgggg
    31921 cgactggcag catacggaaa cccagtatgc cgcacagggt aatggcgacc gacagtaaaa
    31981 tcgtcgccac cgggcggtaa atgaagaggg caaaaaactt cacttacgcc tcctcttcat
    32041 gacgggcaaa gcggcttttg gtccacaatg ccagacggtc gaacagcaga taaatcaccg
    32101 gcgtggtaaa cagcgtcagc acctggctga caatcagacc gccgaccatg ccgataccta
    32161 acggacgacg cagttccgcg ccgaccccgg tactcaacat cagcggcagc gcaccaagca
    32221 gagccgccag agtggtcatc aggatcggac gaaaacgcaa cagacaagcc tggtaaatag
    32281 cttcgcgcgg cgacatgcct tgctcgcgct cagcagccag cgcgaagtcg atcatcatga
    32341 tggcgttctt cttcacgata ccgatcagca aaataatgcc gataatggca atcacatcca
    32401 gttcgctacc ggcaatcatt aacgccagca gcgcgccgac ccctgcggtg ggtagcgtcg
    32461 agagaatggt gatcgggtga ataaagctct cgtacagaat gccgagcacg atatacatcg
    32521 ccaccaccgc cgcgacaatc agccagacag tgctgcccag cgccgactgg aaggcgaggg
    32581 tgctgccctg gaactgcgtg gtgatatcca ccggcagatt cagcgtcttc tctgtgtcca
    32641 taatcgcctg caccgcatcg cccagcgaat agttatccgg cacgttaaag gagatagtcg
    32701 ttaccgggaa ctgatccaga tggttgatgg aaagcggcgc aaaacgctgc tcaattttgg
    32761 caattgagct taacggcacc acgccaccgt cgctgctggt caggcgaatg gtatccagcg
    32821 ccgcgaggcc tggggtattt tcggtgttgt gttccagtac cacgcgatac tggttagcct
    32881 gagtataaat agtggaaatc agtcgctgac caaacgcgtt gtacagggcg ttatcgacat
    32941 ccgccatgct gatccccagg cggctggcgc tgtccctgtc gacattgaca tacgccacca
    33001 gccctttgtc ctgccagtcg ctggagacat cagaaagctg tggcagctgc tggagttttt
    33061 ccatcaactg tggcacccag gtactgagcg catccagtga cgtggcctgc aaggtaaact
    33121 ggtactgggt gcggctgacc tgagtatcaa tagtcaggtc ctgcgttggt tgcaggaaga
    33181 gatcgacgcc cggcacttta tccaccgccg tttgcagacg ggcgatgact ttttgcaccc
    33241 ggtcatctcg ttcatccagt ggtttgagat tgatctgtaa acgcgcactg ttcagcgacg
    33301 ggttagtgcc atcaacgcca acaaatgagg tcaaactttg cactgccgga tcctgcaaaa
    33361 tcacgtccgc gacctggcgt tgccgctggg ccatattcgc aaaggagctg gattgcggtg
    33421 cctgcaaagt gccctgaata atgccgttgt cctgcaccgg gaagaaacct ttcggaatga
    33481 atacccacag cagcacgcta agcagcagcg tgctgagcgc cacgcttaag gtcagccacg
    33541 gatgattcag cactttcgcc agtccacgac cataggcggc gattatcctg tcgaacattt
    33601 tctccgaggc acgggagaag cggttctgtt tacgcagcga ctcctggctg agcatccgcg
    33661 cgcacatcat cggtgtcagg gtcagcgaca ccaccgccga gatcaaaatc gcgactgcca
    33721 gggtaatagc aaattcgcgg aacagtcgcc cgacgatatc gcccataaac agcaggggga
    33781 tcaacaccgc aatcagcgag aaggtcagcg agataatggt aaagccgatt tcacccgcgc
    33841 ctttgagcgc cgccgccaac ggtttttcgc ctttttcgat atagcgggaa atgttctcga
    33901 tcaccacgat ggcgtcatcg accacaaatc cggtggcgat ggttagtgcc atcagcgtca
    33961 ggttattgat tgaaaaatcg agaaacacca tgaccgcgaa ggtgccgatt aacgacagcg
    34021 gcactgcgac gccgggaatg atggtcgccg gaatattgcg caaaaacagg tagataatca
    34081 tgactaccag cgcgatagcc atcatcagct caaactgggt atcgtcgacc gatgcgcgga
    34141 tattggtggt acggtcagaa agtaccgtca ccttcaccga tttcggcaga ctctcggtga
    34201 gctgtggtag catctgccgg atgctgtcgg cggtggagat aatgttagca ccgggctggc
    34261 gctgaacatt catcacaatg gcctgttctt tgttcgccca cgcgccgagc cagctgtttt
    34321 ctgctccttg ctctacagta gcgacatcgc caagacgaat tggtgcgccg ttctgataag
    34381 cgataatcaa ctgacgatac tcttctgccg attgcatctg gtcgttcgcg gaaagcgtga
    34441 ccgcgcgaga agggccgtca aggctccctt tcgctgagtt aacgttcgcg ccagtaatag
    34501 cggtgcgcac actttcgctg gtcaggccga gagcggcaat tgcctgagcg ttgagtttta
    34561 cgcgtaccgc cggacgctgc ccgccggaaa gtgtgaccag ccctacgccg gaaatctgcg
    34621 agattttctg cgcgacgcgg gtttccacca tatcttccac ctgcgtcatc ggcatggcgg
    34681 ttgaggtgac agcgagcgtc atgatcggtg gatctgccgg gttcactttg ctgtaaaccg
    34741 gcgggttagg cagatcgctc ggtagcaggt tggtcgcagc gttaatcgcg gcctgcactt
    34801 cctgctcggc gacatccagc ggcaatgtta gctggaactg taaagtgata actgacgcac
    34861 cgccggaact ttgcgacgac atctgtttca ggccagacat ctgcccgaac tggcgttcga
    34921 gcggcgcggt aacggcagag gtcatgacat ccgggctcgc acctggataa agcgtgacca
    34981 cctgaatggt tggatagtcc acttccggca gcgccgaaac gggcagggcg cgataaccga
    35041 taatcccggc gagtaagatc gccaccatca gcagcgtggt ggcgacagga cgcataataa
    35101 acaggcgcga cgggccgcct gtgctgctcg ggggtaacac ctgcatcagg agcgtgctcc
    35161 ttttttcgcg tattcgcggc tggtggcttt ctcttccgga gtagtggcgc tctgggcttc
    35221 caccacttcc actttcgccc cttcggtcag gcgatcaatg ccgtccgtca ctacgcggtc
    35281 gcccgcagaa atacctgcgc ggatcaccac tttctgactg tcctgaatgc ccggtgtcac
    35341 cagatgtttg ctgactttgt tttcgctatt cagtacccag acaaaatggc cttcgttgcc
    35401 catttgcaga gcggcggtgg ggatcaccac ggcgttttgt tcggtgtcga ctaacatgcg
    35461 cgcgttaaca aactgattgg gaaacagcgc atcatcctga ttattaaagc gtgctttcac
    35521 tttaatcgta ccggtagtgg catcgatttg gttatcaagg cttaacagcg tgccttcact
    35581 taacttcttc gagttggtgc gatcccaggc ttctaccacc agcggttttc cggctttttg
    35641 tgcctgtaca acggtggcga tatcgctttc cggcagagta aagaccagat cgataggatg
    35701 tgtttgggtg atcaccacaa tcccggtggt gtcgccactg gagatttggt taccaacatc
    35761 aacctgcttg agaccaacgc gaccatcaac tggtgcggtg atacggctcc agtcgagttg
    35821 caattgcgcg ctggcgacgc ttgcttcatc agccttaatg gtaccttcgg tttcactgac
    35881 cagcgcttgt tgggcatcca gttcctgacg agatacgaga ttggtttttg ccagttgttg
    35941 ataacgcgcc aggtcacggc gggcgttggc gagcgtggct ttatcttttg ccagttggcc
    36001 ctgggcttgt gctaatgcaa ctttgaactg gcttgggtca atttctgcca gtaaatcgcc
    36061 tgctttgacc tgctggcctt cctggaaatg taacgccatc agttgaccgt ccacgcggct
    36121 gcgcaccgta acagtattag cggcggtaat ggtgccaagc ccggtgaggt aacgcggaac
    36181 agcctgttct actgcggtcg ccgcctgaac cggagctaat gggccggcac gcataccacg
    36241 gcgaccaccc gctggcgatt gctgcgcttg tttcgtcgcc cctggggttg cactctggga
    36301 gtcattgcgg ccttgccaga accagaatgc ggcgatggcg gcgataacca ccacgattac
    36361 gattacccaa cgggatttat aactgccttt catcgttaag agtttctctt cctgaaacgt
    36421 ttcgcggaat gatactagtg tagccagcca ataagggaga aaaatggagg aattatgaaa
    36481 cactgtcggg aatcgtctga atgacgggca cttttgcgag cacgcatcca gtaataacac
    36541 atgaaactat tttatctacg cgttagcgat agattgcttg catggcgaaa ggagataagc
    36601 cgacgatttc agcgggacgc tgaaacggga aagcccctcc cgaggaaggg gccttaaata
    36661 aggaaagggt catgatgaag atactcatca tcgtggtgct cttagtgatt agctacccag
    36721 catactaaga ctaccagggc aggggaaacc ccgctctacc ctcactcctg aaagtatgcc
    36781 ttcacggtcc aattgtcaat ccgcagactt tgcagtctgc gatcctgcca gcaaatattc
    36841 tttgcgagtc gttacgcaat aatcacactt gaaactattt tatccgcaag tcagcgatag
    36901 actgcataca gggcgaaagg aagtaagccg aagatttcag cgggacgctg aaacgggaaa
    36961 gcccctcccg aggaaggggc cttaaataag gaaagggtca tgatgaagct actcatcatc
    37021 gtggtgctct tagtcattag ctggccagct tactaagact accagggcgg gggaaacccc
    37081 gctctaccct tactcctgaa attatgtcac tccgccctta gtgtcaatcg acggctctcc
    37141 gttcttgcga ggcgaatagc aaaattttct gcgccgccgg gcctaattct gaaagtttct
    37201 tcgtttgctc ttatttcatt attgcaatcg gtatgatacc ggacctcaat aattctctaa
    37261 gaaaggattt cttatgagcg aacaaatcac gtttgccacc agcgattttg ccagtaaccc
    37321 ggaaccacgt tgcccctgca ttttgctgct ggatgtttct ggttctatga acggtagacc
    37381 cattaatgaa cttaacgccg gattggttac ctttcgcgat gagctgcttg ccgattcgct
    37441 ggccttaaaa agagtggaac tcggcattgt gacgttcggc ccggtgcatg tggaacaacc
    37501 ctttaccagc gccgctaatt ttttcccgcc catcctgttt gcccagggcg atacaccaat
    37561 gggcgctgcc attaccaaag ccctggatat ggttgaggag cggaaacgtg aatatcgtgc
    37621 caatggtatt tcctattatc gtccgtggat tttcctgatc accgatggcg caccaaccga
    37681 tgagtggcag gctgccgcca acaaagtgtt tcagggggaa gaagataaga agtttgcctt
    37741 ctttaccatc ggtgttcagg gggcggatat gaagacgctg gcccaaatca gcgtccgtca
    37801 gcctttgtct ctgcagggat tacaattccg tgaactgttt agctggttat ccagttcgct
    37861 gcgttcggtt tcccgctcca cgccgggaac ggaagttgtg ctggaagcgc caaaaggctg
    37921 gacgtcagtg tgagctggcg actggtctat gcatcagccg ttggaacgtc gcatattagc
    37981 gctgatttgc cttgccagga tgcctgccag atgcaggttg cctggctaaa tgaccagcag
    38041 ccattattgt cggtatttgt cgctgatggc gcaggcagcg tctcgcaggg tggcgaaggt
    38101 gcgatgctcg ccgtcaatga agcgatggct tatatgtcgc aaaaattgca gggcggggaa
    38161 ttggggctta atgatgtcct cgcaacggac attgtgctga ccattcgtca gcgacttttt
    38221 gccgaggcgg aagccaaaga attagcggtt cgcgattttg cctgcacgtt tttgggattg
    38281 atttcatcag ctaatggcac cttgattatg cagattggcg atggcggcgt ggtggtagat
    38341 ttggggcacg gtttgcaact gccgctcacg ccgatggtgg gtgaatatac caatatgacg
    38401 cacttcatta ccgatgaaga tgccgtttcc agactggaaa ctttcaccag cactgagcgt
    38461 gcgcacaaag ttgcagcatt tacggatggt attcagcgac tggcgttaaa tatgctggat
    38521 aattctccgc atgtgccttt ctttaccccg tttttcaatg gactggcgtc agcaacgcag
    38581 gaacaactcg atttattacc tgaattgtta aagcagtttt tgtccagccc ggcggtaaac
    38641 gagcgtacgg atgatgataa aacgttggca ctggcgctgt ggttaccatg aaacccactt
    38701 tatatactgc tactggtgag tgcgttacgc caggccgtga actgggcaaa ggcggcgaag
    38761 gtgcggttta tgatatcgag gagtttgtcg atagcgtcgc caagatttat cacacgccgc
    38821 cacccgcctt aaaacaggac aaacttgcct ttatggctgc gacagctgac gcgcagttgt
    38881 tgaattatgt cgcctggccg caggcaacgc ttcacggtgg acgaggcgga aaagttatcg
    38941 gttttatgat gccaaaagtt tctggtaaag aaccgattca tatgatctat agcccggcac
    39001 atcgtcgcca gagttaccct cattgtgcgt gggattttct actctatgtt gcgcgcaata
    39061 ttgcttcatc ttttgctact gttcatgagc acgggcacgt cgtgggtgac gtaaaccaga
    39121 acagctttat ggtaggtcgc gacagcaaag tggtgttgat tgatagcgac tcctttcaga
    39181 ttaatgccaa tggtacactg catttatgcg aagtcggcgt gtcgcatttt acgccgccag
    39241 agctgcaaac catgccatca tttgtcggct ttgaacgcac agaaaatcac gataattttg
    39301 gccttgcgtt gctgatattt cacatcttgt ttggtgggcg gcatccttat tccggtgtac
    39361 cgcttatctc tgatgcaggt aatgcgctgg agacggatat tgcccatttc cgttatgcct
    39421 acgcctcaga taatcagcga cgtggtttaa aaccgccgcc acgatcgatt ccgctgtcga
    39481 tgttaccggg cgatgttgaa gccatgtttc agcaggcatt cacggaaagt ggcgtggcaa
    39541 ccgggcgtcc gacggcaaaa gcgtgggtag cggcacttga tttactacgc caacagttaa
    39601 agaaatgtac cgtttcggca atgcatgttt accccgctca tttgactgac tgcccgtggt
    39661 gtgcgctgga taatcaaggc gttatctatt ttattgatct cggcgaagag gtcattacca
    39721 ccggcggtga ttttgtgctg gcgagagtct gggcgatggt gatggcatca gtagcaccgc
    39781 cagcattgca actgccatta cccgatcatt tccaaccgac tggcaggccg cttccttcag
    39841 gcctgttacg gcgcgaatac atcattctga ttgagatcgc actgtcagcg ttatcgctgt
    39901 tgctttgcgg ccttcaggca gaaccgcgtt atattatttt gattcctgtg ctggcggcta
    39961 tctggattat tggcagcctg acaagcaaag cgtacaaagc agaaatccag caacgccgtg
    40021 aggcgtttaa tcgcgcgaaa atggactatg accatttagt cagccagatc cagcagttgg
    40081 gcgggctgga aggttttatc gccaaacggg cgatgctcga aaaaatgaag gacgaaattc
    40141 tcgggttacc ggaagaagag aaacgcgctc tggtagcact tcacgacacc gcaagggaac
    40201 ggcagaagca gaagtttctg gagggatttt ttattgatgt tgcctctatt cccggcgttg
    40261 gccctgcgcg taaagcggcg ttacggtcct ttggtattga aacagcagcg gatgttaccc
    40321 gacggagcgt taagcaagta aaaggttttg gtgatcatct gacccaggcg gttatcgact
    40381 ggaaagcgag ctgtgaacgc cgttttgtgt tcaggccgaa cgaagcggta acgcctgcag
    40441 acagacaagc ggtaatggca aaaatagccg ccaaacgaca tcggctggaa tcggcgctga
    40501 ctgtcggcgc gacagagttg cagcgattcc gccttcatgc gccagcacgg accatgccgt
    40561 tgatggaacc gctacgtcag gcggcagaaa aactggctca ggcgcaggca gatttaagcc
    40621 gttgctgagc catttttcaa ttaacgaaac accacttccg cccagcgtgc cagcccggcg
    40681 gtgacggagc caaagtcatc gccgcctgca accggaatgc ccggcaactg ttctgccagc
    40741 gcttttttaa tcagcggcga acgggcgcta ccgccggtca ggtagataac gtccggtttt
    40801 tcctgggcgt tatccagcgc cagttgcacc tgttccagaa tccgcgccag tggctggctg
    40861 agggcgcttt ccagcccttg ctggctaatc agcgtagcca gttcatcgct gataaacggc
    40921 agtgaggcgc gggtttccgc tacacttgaa agggcgattt tgctctcttc tgcgctacgc
    40981 accaggcgat agcttaaacg ctgacgccag actttctgta acagggccac tttttccggt
    41041 tcacgggcat cgcgtaccag atcgttaagc agacgaccgt tagcactact gtagaaatca
    41101 ctttgcgcag gaacgtcgtt gatcgcaacc gcattccacc acggcagaat cggcagggcg
    41161 atgccttttt cggtttcgcc acccatgccc agtaacggca tcaggttctt aaacgccagt
    41221 gcgatatcca gatcgttacc accaatacgg caaccactat gacccagcag gctggcttca
    41281 cgatcgagac gcgaacgcca ttgcggcccc atcagcagca atgaacagtc agtcgtacca
    41341 ccgccgatat ccactatcag cactcgtttt tcttcctgca aggtggcttc gtaatccagc
    41401 ccagccgcga ccggctcgta ctggaatacc acatccttaa acccggcacg cttcgccgcg
    41461 cgttccagaa tcccttgcgc ctgggtgttt gcttcatcac cgcccagccc ctggaagttg
    41521 atcgggcgac caatcaccgc ctgagtaatt gcctccggca gctgcgcctg cgcctgttgg
    41581 cgaatgtgca acatcattgc gcagaccaga tcctcaaaca gcgctacctg ctgcggtttt
    41641 aagccgctgg caccgaggaa cgattttggt gatttcacaa accacacttc ttctggatca
    41701 tcaatgtact gtgccagtga ggaaagaccg aactgcacgc ttttcgccgt aacatcgata
    41761 tcttcttcgc ggttataacg aatcgcccga cgcaacagcg cctgcgtttc atcgtcgtct
    41821 gccggaacat catgatggcg gtacagccat tcgcttacag cttcacgcgt tggcgcgcaa
    41881 agcattgaag gcagcagcgt gctgtcgttt tccattttta gcaaatgcgg tttaccgtcg
    41941 cgcatgaccg ccactgaaca gtttgctgta ccgtaatcaa aaccaataaa cacgaaataa
    42001 tccccatgcc ggtgaagaag gggcgtgact ttagcgaaat gttgccgtcg cgacaaccgg
    42061 aatatgaaag caaagcgtaa cgtctgaata acgtttatgc tgaaagcgga ttaacaagga
    42121 gatgcgatgt ataccctgaa ctggcagccg ccgtatgact ggtcgtggat gttgggattt
    42181 ctcgccgccc gtgcggtgag cggcgtggaa acggtcgcgg acagttatta tgcccgtagt
    42241 ctggcggtag gcgaatatcg cggcgtggtg actgctattc cggatatagc ccgccatact
    42301 ctgcacataa atttaagtgc aggtttagaa cctgttgccg ctgagtgtct ggcgaaaatg
    42361 agccgcctgt ttgatctgca atgtaacccg cagattgtta acggtgcgtt gggcaagtta
    42421 ggcgcggcgc ggccaggatt gcgtttacct ggttgtgttg acgctgttga gcagggcgtg
    42481 cgggcgattt taggccaact ggtgagcgtg gcgatggcgg caaaattgac ctccagagtg
    42541 gcacagcttt atggcgaacg gctggatgat tttccggatt atgtctgctt ccccacgcct
    42601 cagcggctgg cagtagccga cccgcaggca ttaaaagcgt taggtatgcc gttgaaacgg
    42661 gcagaggcgc tgattcatct ggcaaattcg gcgctggagg gcaccttacc aatgacaata
    42721 ccgggcgacg tagaacaagc gatgaaaacg ctgcaaacct ttccgggtat cggacgctgg
    42781 acggcaaatt attttgcttt gcgtggctgg caggcgaaag atgtttttct gccggatgat
    42841 tatctgatta aacagcgatt tccgggaatg acactggcac aaatccgccg ttatgccgaa
    42901 cgctggaagc cctggcgttc ttatgcgctg ttgcatatct ggtatacgga aggctggcaa
    42961 ccagacgaag catgacgttg gtcgccggat gcggtgcgag cgccttatcc ggcctgggga
    43021 atgagcgatt tgtaggcctg ataagacgcg ttagcgtcgc atcaggcaat gggcaaacgc
    43081 tcccttagtt aatcgcgaaa taacttctat tcaccagcaa atccagcggt tgggcattgg
    43141 caatcacatc accataaatc agatcgacgc cgatgcccga aagcgtatcc atcactaagg
    43201 gtaaaacgac tggcccggcg atggttttca tcccgagtcg ctgagcgtgc ccctgaataa
    43261 tcgtaatcag catctcatcc atcaaattac cctgcacgtt ggcgcataac tcaccatcaa
    43321 gtagcaggta atctgccata tttgctttca gcgagttgaa gatttgcaga tcgcggccta
    43381 cctgactgaa tacgatccga catcccgcca gccgcagttt ttgcacgctt tctgcgtgat
    43441 cgaaaatcgc ttctgccgga ataatcagat gtaataaccg tgctggtaga gggctatttt
    43501 ccagctgctc aagcagttca ttcaccagcg tggcgctact caaaccagca acggaaaggg
    43561 gaagggcgat gcttaagcct ttactggcaa ccgcttttgc ggcctgctgg aaaaaatcgt
    43621 ggcatacccg ttggtcaaga gcatggctaa gtgccggatc gctgaagcta cgacgaaatg
    43681 tttgttcatc aataatctcg ctttcgcaac tccagagctt aagtgaaatc agccacaaat
    43741 tacttgcttc cgggagccgt ggcgaagcga tgccgtgggc gatcatcatc aactgattct
    43801 ctttaatcat ccgccactgt tcatcaagcg atatcgctgc ccgttcgcta tgtgcggcag
    43861 cttgctgcgg ttcgtaaatc gtcacccggc cccggccacc atttttggag gcataacagg
    43921 cgatatcagc ctgcgacatc acttcagctg cctgatgatt gttgtcatca atcaaggtaa
    43981 tcccggcact ggcacctacc cgatgcacac gtccttccca tataaagtga tagtcattca
    44041 cggcactgat aatgcgtgta gcgataaaac gagcgctttc aacattgcag tctggcagta
    44101 gcagaccaaa ttcatcacca ccgagccgcg ccagcacgtc gctggagcgc agcatactca
    44161 gcatcaacga cgccagttcg cgcagtaaag cgtcgcccgc cgcatgcccg gcgctgtcat
    44221 tcaccgcttt aaagcgatca agatcgataa acaccagggc atgtcgctga tgcgtactgt
    44281 ttaccgtttg cagcaggata cgcagttgct tctcaaaact ggcgcgattg gcgagatgcg
    44341 tcagtgcatc atgggaggcg ctgtagctca gctggcgcag cattttgcgt gattcggtaa
    44401 cgtcctgaat caccagtacc gagcctatat tgctgccgtc cagagtactt aacggcgtaa
    44461 tactgtaatg cacgtcgtaa ctgccgccgc tccggcagtg caataccaca tcttgttcga
    44521 tcgcggaacg tgaggtgtca gcactgtaaa tgttctccat taatggtccg ttgtcgccaa
    44581 aagtaatatg caacaccgtc aggagcggaa cacctaacgc ttcttcctgc gtccagccgc
    44641 tcatcttctc cgccactgga ttcataaagg taattttcat tgccatatca atacagacca
    44701 cggcttcgcc gatggagtcc agcgtaatgt gcaggcgctc tttttcctga aacaatgcct
    44761 cgttaagctg tttaacctcc gtcatatcca tattaatgcc gagcaggcgt tcaacttcgc
    44821 cttctttatt cagtacccgg ttggcgaggg cgcggatatg gcggatgcca tctttcacgg
    44881 caatgcgaaa ttccagttta aagggcgagc gtgattgcaa cgaatcacga atcacttttt
    44941 cggcgtgctg gcgatcttcc ggcagcacgc actcgtacca cacctgccag ttcggtttga
    45001 tatgcggagg aatttcatac agctcgaaca tccgcttatc ccaactaaaa atattcggct
    45061 tcagctccca ctcccagatg ccaatcccgc ccgcttcgtt ggccagcgtg atgcgctcca
    45121 tcagttgttg attgacctgt tcggtgcgtt ttagctcgtt aatgtcttca atctgagcga
    45181 taaaatagag cggcgtgcca tccgtgtggc gcaccagtga gacggcaagc aacgcccaga
    45241 caacatcgcc attgcggttg tagtaacgtt tttccattga ataggtgttt atttcaccgc
    45301 ttatcagctt ttcaacctgt tggagatctt tattgagatc ctccggccag gtcagttgct
    45361 gaaaggtgag tccgcgcagc tcttcctgac tgtaaccgag aaactggcag agcgctttgt
    45421 tggattgcag ccattgtccc tcggtgccca ctaatgccat gccgatggcg gaatattcca
    45481 tggcgttccg aaagcgggtt tcgctttcgg aaatgtgttt gcgttccgca cgaaaggcat
    45541 acatcaccat cgtcatgatg ttggcgggca gcaggatcag caaaaacggt agccacggca
    45601 tatggctcat caggtacgta cgcggcgtag caagcaggga gggatccgcg gccatcatca
    45661 gcgacaccat catcaccgtg gtaaggaaga tcaaaaaggc ttccattcgt ggcaggcgca
    45721 cggcgctcca catcaacagc acaataatga aagtaaaagg ccacggcaga tacagcatcg
    45781 aaagccagct taacgtcagc gtgatggcta acgtgagcag cgactcaaaa agcaaccttg
    45841 ggttgcgatg gcgcagtaga tagtgtggtt taaataacaa tcccagcggc accagtgcca
    45901 gagcgccgat ggattctgac agtacccata tcaaaaatgc cctgagagga tcgtctccgg
    45961 gcgtcagcag gacaaccaga acacccccca acagaggcgg aataatggcg ctgccgagtg
    46021 ccagacgcag ccagtcagcc agattttgca aggggttata ccacggcagc aatttacgta
    46081 gcagcactgc cccgaccacg gcttcaacaa tattgatggt cgtccaggtc atgttcagcg
    46141 agctggtgga aaaaagcagg atggatgcgg cgatatttcc cagcgagcag ctcagcgcaa
    46201 ttcccggcca catgcgcccg gcatggcgat aaaacgccac catcatgatg gacgtcggga
    46261 accacagtgg ggcgagttgg gtgccaaact gcgaaagctc aagtgagaaa agggtgaaga
    46321 taaacgagac taaacctaaa ctgaccaggt gaagcagcgg gtggggcagg gcaattaata
    46381 catgctgtga ttgtttgctc attaccgctt tatccgcaag gccaggtcgc cagtattcgt
    46441 ttctgtgttt atgctagtac aaaagtttta cattttatat gcgaattgct cataaagtga
    46501 cgtaaaggcg gataatttgc gcaactgcgt ataacatttt ttaccttgta taaaactgat
    46561 caacgtaatt tgccaccggg aattcttcgt caacaattaa ctctgagcgc gcggcaacgc
    46621 tattcgactg gtatcaggcg gatgaaatcc ctataattgc cgcgtttggc gcttcgtcgc
    46681 ccccttccta acatccaggt taatcaggtc gctaaatata tgactgatca gtctcatcag
    46741 tgcgtcatta tcggtatcgc tggcgcatcg gcttccggca agagtcttat tgccagtacc
    46801 ctttatcgtg aattacgtga gcaagtcggt gatgaacaca tcggcgtaat tcccgaagac
    46861 tgctattaca aagatcaaag ccatctgtcg atggaagaac gcgttaagac caactacgac
    46921 catcccagcg cgatggatca cagcctgttg cttgagcatt tacaagcgtt gaaacgcggc
    46981 tcggcaattg acctgccggt ttacagctat gttgaacata cgcgtatgaa agaaacggtg
    47041 acggttgagc cgaagaaggt catcattctc gaaggcattt tgttgctgac ggatgcgcgt
    47101 ttacgtgacg aacttaactt ctccattttc gttgataccc cgctggatat ctgcctgatg
    47161 cgccgcatca agcgtgacgt taacgagcgt gggcgttcaa tgaattcagt gatggcgcaa
    47221 tatcaaaaaa ccgtgcgccc gatgttcctg caattcattg agccttctaa acaatatgcg
    47281 gacattatcg tgccgcgcgg cgggaaaaac cgcatcgcga tcgatatatt gaaagcgaaa
    47341 ataagtcagt tctttgaata agcttgataa attgtgtacc gttcagtgat aacctggtat
    47401 gcccttgacg taattggcgt taagggagtg atgctcgaaa ggagaaaatg ccatgcgtct
    47461 gtgtgaccga gatattgaag cctggcttga tgaaggccgt ttgtcgatca acccacgtcc
    47521 gccagtggag cgtattaacg gcgcgacggt ggatgtgcgc ctgggcaata aatttcgtac
    47581 cttccgtggt cacacggcag cgtttatcga tctgagcggt cccaaagatg aagtgagcgc
    47641 cgcgcttgac cgcgtgatga gcgatgagat cgttctcgat gaaagcgagg cgttctatct
    47701 tcacccagga gagctggcgc tggcggtgac gctggagtcg gtgacgctgc cagccgatct
    47761 ggtgggatgg ctggacgggc gttcctcact ggcgcgtctg gggctgatgg tgcacgtcac
    47821 cgcgcaccgt atcgatccgg gctggtctgg ctgcattgtg ctggagttct acaactccgg
    47881 taagctgccg ctggcgctgc gtccgggcat gttaattggt gcgctgagct ttgagccgct
    47941 ttccggcccg gcggcgcgac cttacaaccg ccgtgaagat gcgaaatatc gcaaccagca
    48001 gggcgcggta gccagccgaa tcgataaaga ctaattcagg cccattgagg ataccatgag
    48061 acgatttctg acgacgctga tgatactcct ggtcgtgctg gtggccgggt tatctgcgtt
    48121 agtgttgctg gtgaatccga atgatttccg cgactatatg gtcaagcaag ttgctgcacg
    48181 tagcggttat caattgcagc tcgacgggcc actgcgttgg cacgtctggc cgcagcttag
    48241 tatcctctcc gggcgaatgt ctctcaccgc ccagggcgcg agccagccac tggttcgagc
    48301 agacaacatg cgtctggacg tggcgctttt accactactg agccatcaac tgagcgttaa
    48361 gcaggtgatg ctaaaagggg cagtgatcca actgacgccg cagacggaag cggtgcgcag
    48421 tgaagacgct ccggttgcac cgcgcgacaa taccttgccg gatctgtcag acgatcgcgg
    48481 atggtcgttt gatatatcca gtcttaaggt ggcggacagc gttctggtgt tccagcatga
    48541 agatgacgag caggtgacaa tccgcaatat ccgcctgcaa atggaacaag atccccaaca
    48601 tcgtggctca tttgagtttt ccgggcgggt taatcgcgat cagcgcgatc tcacgatatc
    48661 ccttaacggt acggtggatg cttctgatta tccgcatgat ttaatggcgg ctattgaaca
    48721 aattaactgg cagttgcagg gtgccgattt accaaaacaa ggtattcaag ggcaggggag
    48781 tttccaggtc cagtggcagg agtcacataa acgcctttca tttaaccaaa ttagtttgac
    48841 cgccaatgac agtacgctga gcgggcaagc acaggtcacg ttgacagaga aaccggaatg
    48901 gcagctgaga ctgcaattcc cgcaactgaa tcttgacaac ctcatccccc ttaatgaaac
    48961 agcgaatggt gagaacggtg ccgcgcagca agggcagagc caatcaacgt tgccgcgccc
    49021 ggtcatttct tcgcgtattg atgaaccggc ctatcaggga ctgcaaggct ttacggctga
    49081 tattttgttg caggccagta acgtgcgctg gcgcggaatg aattttacag atgttgccac
    49141 gcaaatgacc aacaagtcgg gtttgctgga aattactcaa ctgcagggca aacttaacgg
    49201 tggacaggtt tcactgccgg gcacgttgga cgcgacatca ataaatccgc ggataaactt
    49261 ccagccacgg ctggaaaacg ttgagattgg taccattctg aaggcgttta actatccgat
    49321 ttcgttgacc ggaaagatgt cactggctgg tgatttctcc ggtgctgaca tagatgccga
    49381 cgcattccgc cataactggc aaggacaggc acatgtcgaa atgaccgaca cgcgcatgga
    49441 agggatgaac ttccagcaga tgattcagca agcggtagaa cgtaatggtg gtgatgtgaa
    49501 ggccgctgaa aacttcgata acgtaacgcg tcttgaccgc tttaccaccg atttgacgtt
    49561 gaaggatggc gtcgtgacgt taaacgacat gcaaggtcaa tcgcctgtgc tggcgctgac
    49621 aggggaaggc atgttgaatc tggcggagca aacctgtgac acccagtttg atattcgggt
    49681 cgtgggtggc tggaacgggg aaagcaaact gattgatttc ctgaaagaaa cgccagtacc
    49741 gctgcgggtt tatggcaact ggcagcaact taattacagc ctgcaagtgg atcagttact
    49801 gcgtaaacat ctacaggatg aagcaaaacg ccgtcttaac gactgggccg agcggaataa
    49861 agattcccgc aatggcaaag atgtgaagaa gttgctggag aagatgtagg ttaccaggtg
    49921 acctgtcctg taggcctgat aagacgcgta aagcgtcgca tcaggcctac atgccatcac
    49981 acctcgtact ccatctcgcc atccttatgc agcggaataa tctgtacctt ctgcacgcga
    50041 tggctttcca cctgcaacgt tttaagcaaa taatccccca cctgaacttc ttcgccaggt
    50101 tttggaatac gctgcaaata ttccatcaat agcccggcaa tggtgtgata ttcacgtttt
    50161 tcatccagcg gcagcggcac atattgcacc agatcttcca gcggcatatg gccattcgcc
    50221 gtccaggaac cgtccgcatt cttctgaata tcatggcggg cgtcgatctc ttccacttcg
    50281 ttcggtaagt taccggcaat ggtttcagtg acgtcactta atgtcacaat cccttccacc
    50341 gagccaaact catccaccac aaaagcaaag tgtgtgcggg cattacggaa ctgctccagg
    50401 gcaggtaaca acggcaaggt ttccgggaac accagcggct gacgaatcaa cacccgcagg
    50461 ttgagcggtt cgccgcgcag tgactgttgt aacaggtcga taacgtgaac aacaccgagc
    50521 aaatcttctg cgtcatcgcc atcagtaaca acaagccgcg tatgctgatt tcgctccagt
    50581 aactggcgga tctcgtcttc cggcgcgttg agatcgatat gctcaatatc gtggcgcgat
    50641 gtcataatgc tgctgacggt acgctggtta agattaagta cccgctcaat catccgccgt
    50701 tcctgcggat taaagatctg ctggttacca tgatccacca gcatggacgc ggtttcggcg
    50761 tccagttccg catcttcttt ttgcccgcta agcaggcgca ttaccgcttc ggtagtccgc
    50821 tggcgcagtg tctggttagc ggaaagaaaa cgtcgtcggt taaagatagc caactgattg
    50881 agtgcctcga tcatcaccga gaaaccaatg gcagcgtaca ggtagccttt cggaatgacg
    50941 aagccgaaac cttccgccac caggctaaag ccaatcatca acaggaagct taagcagaga
    51001 ataacgatcg tcgggtgact gttaacgaac tgggttaacg gcttgctggc catcaacatc
    51061 aggctgatag cgataaccac ggcggccatc atgaccagta aatggtcgac catcccgacg
    51121 gcggtaatca ccgagtcgag tgagaagatg gcgtccagta ccacaatttg tgtcaccacc
    51181 ccccagaact tcgcaccttt gcgttgggtg gggttattgc tgtctttccc ttccagccgt
    51241 tcgttcagct ccatcgtggc tttgaacaac aggaagaaac caccaaacag catgattaaa
    51301 tcgcgggcgc taaaggtaaa cgagcggaag ctgaacagcg gttgagtcag ggtgaccagc
    51361 caggagattg acgccagcag taacaggcgc attaacatcg ccagcaggag cccggtaacc
    51421 cgtgcgcggt cgcgctgctt aggcggtagt ttttcggcga ggatggcaat aaagaccagg
    51481 ttatcaatgc cgaggaccag ttcgatcaca atcagcgtga ttaacccagc ccagatagac
    51541 ggatcggcaa tccattccat agtaagtaca aaacctattc gttgtatgac aaatgtcaca
    51601 attcgatcat ggataaatcg gggcaaaaat gcaatgttgc agcactattt atctcatatt
    51661 atttgagggt tataacctca cattatcccc gaattataag tggtaataat aaagcgttat
    51721 ttaagaaata tcgcatgtgg aaacgccata ttaaatattg tcaacctaaa gaaactccta
    51781 aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc cgaattgtta
    51841 ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag agccaatatt
    51901 caattaccat aatgtcacct tatattcgga aattaatttc cgcaataacc ctttctcatc
    51961 aacgactgta tgcagagagg gtttgaaaaa ttaagctaaa tcagtgtatt ggtagctaaa
    52021 aagccagggg cggtagcgtg tctggatgcc tgagagacca gtctgaatta ttctgtcaat
    52081 agcctgcgga tgaaatcaat ttttttagga ctgatgcctg ttaaattttt attcaattaa
    52141 ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata attactctgc
    52201 caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt attggtgtca
    52261 gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac gatgggtaaa
    52321 gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa tgtttatccg
    52381 ctgaccccgc gcctgattga ccaattacgc ccacgcccca acgtagcgcg ccccaatatg
    52441 acgctggaaa gcgagatcgc gaattaccag tatcgcgtcg ggccggggga cgttcttaat
    52501 gtcaccgtct gggatcaccc ggagctcacc acgccagccg gtcagtaccg cagctccagc
    52561 gacaccggca actgggtaca gcctgacggc accatgttct acccgtatat cggcaaggtt
    52621 cacgtcgtgg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg cttagcgacg
    52681 tacatcgctg atccgcaggt ggacgttaat atcgccgcct tccgctcgca aaaagcctat
    52741 atctccggtc aggtgaataa atccggtcaa caggccatta ccaacgtgcc tctgaccatt
    52801 ctcgacgcta tcaacgccgc aggtggcctg accgacaccg ctgactggcg caacgtggtg
    52861 ctaacacaca atggtcgtga agagcgcatt tctttgcagg cgctgatgca aaacggcgac
    52921 ctcaatcaga accgactgct ttaccccggc gatattcttt acgtgccacg taatgatgat
    52981 ctgaaagtgt ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat ggactttagc
    53041 ggcatgaccc tgactgaagc cctgggcaat gctgaaggta tcgacatgac cacctccaac
    53101 gccagcggca tctttgtcat tcgtccgctg aaaggcgaga gcgggcgtaa cggcaagatt
    53161 gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtaatggc gacagaattc
    53221 cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtatcccg ctggaaccgt
    53281 ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga tacagccagc
    53341 gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt gtcggcaata
    53401 tttgccgttc cccgacggcg gaacgcttac tgcaacgtta tcacccggag ctaaaagtgg
    53461 agtccgctgg actcggcgcg ctggtcggaa agggcgctga tcctaccgct atcagcgtcg
    53521 ccgcagaaca tcaactgtct ctggaaggtc attgcgcacg gcaaatcagc cgccgtctgt
    53581 gtcgcaacta cgacctgatt ctgaccatgg aaaagcgcca tatcgaacgc ttatgcgaga
    53641 tggcacccga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac gaatgtgaaa
    53701 tccccgatcc atatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca ttacttgaac
    53761 ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa tgacagaaaa
    53821 agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg gtcgcctggt
    53881 cggcaccatc attgaagcac gctggtgggt gattggcatt accgccgtat tcgccctctg
    53941 tgccgtggtt tacaccttct tcgctacgcc gatatacagc gccgacgcac tggtacaaat
    54001 cgaacaaagc agcggcaatt cgttagtgca ggatatcgga tcggcattag ccaacaaacc
    54061 gcctgcatcg gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg gtaaaacggt
    54121 ggacgatctc gaccttgata ttgcggtgag caaaaacacg ttcccgattt tcggtgcggg
    54181 ctgggatcgc ctgatgggac gccagaacga gacggtgaaa gtgaccacct ttaaccgacc
    54241 gaaggagatg gcggatcagg tgtttacgct taatgtgctg gacaacaaaa actacaccct
    54301 gagcagcgat ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga aaaaagaagg
    54361 cgtcacgctg atggttgaag ccattcacgc cagcccgggc agtgagttta ccgtcaccaa
    54421 atactccacg ctggggatga tcaaccaact gcaaaacagc ctgacggtaa cggagaacgg
    54481 caaagacgca ggtgttctga gcctgactta caccggtgaa gatcgcgaac agatccgcga
    54541 cattcttaac agcatcgccc gtaactatca ggaacaaaat attgagcgca aatcggcgga
    54601 agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta gccgccttga
    54661 tgttgccgaa aacaaactga atgccttccg tcaggataaa gattcagttg atctgccgct
    54721 ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga acgaactgac
    54781 ctttaaagag gcggaaatct ccaagctgta caccaaagtt cacccggcgt accgcacgct
    54841 gctggagaaa cggcaggcgc tggaagacga aaaagccaaa cttaatggtc gcgtaacggc
    54901 gatgccgaaa acccagcagg aaattgtgcg tctgacccgc gatgtcgagt ctggtcagca
    54961 ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag ccagcaccgt
    55021 cggcgatgtg cgcattgtcg acccggcaat cacccagcca ggtgtactga aaccgaagaa
    55081 agggctgatt atccttgggg cgattatcct tggcctgatg ctctctatcg tgggtgtgct
    55141 gctgcgctcg ttgtttaatc gcggtatcga aagcccgcag gtgctggaag aacacggtat
    55201 cagcgtttat gccagcatcc cgctgtcgga gtggcagaaa gcgcgcgata gcgtcaaaac
    55261 catcaaaggg attaaacgct ataaacagag ccagctactg gcggtgggga atccaaccga
    55321 tctggcgatt gaagccatcc gtagtctgcg taccagtctg cacttcgcga tgatgcaggc
    55381 acagaacaat gtgttgatga tgaccggggt tagcccgtca atcggtaaaa cctttgtctg
    55441 cgccaacctg gcggcggtga ttagccagac caataaacgg gtgttgttga tcgactgcga
    55501 tatgcgcaaa ggctacaccc acgagctgtt gggcactaat aacgttaatg gcctgtcgga
    55561 aattctgatt ggacagggcg atattactac cgctgctaaa ccgacctcta ttgccaaatt
    55621 tgacctgatc ccgcgcggtc aggtaccgcc aaatccttct gaactgttaa tgagcgaacg
    55681 ctttgccgaa ctggtgaagt gggcgagtaa aaactacgac ctggtgttga ttgatacgcc
    55741 gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgtcggaa ccacgttgat
    55801 ggtggcgcgt tatgcggtca acacattgaa agaagtggaa accagtctga gccgctttga
    55861 gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc gcgccagcgc
    55921 gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat aacgagatcc
    55981 gctgtcggat gcgatgctga cgcatcttat ccgacctacg ttcacgccgc atccggcaac
    56041 agaacctgta aaccagctta taaaccggca gaacatctcg atactccggg agaacaacct
    56101 atgaaagaca atccgctgat ttcaatctat atgccgacct ggaaccgcca acaactggcg
    56161 attcgggcga taaaatcggt cctgcgccag gactacaaca actgggagat gatcatcgtg
    56221 gatgattgct ccacctcctg ggaacaacta caacagtacg tcaccgccct caacgatccg
    56281 cgtatcactt acattcacaa cgacattaac agcggggcgt gcgcggtacg taaccaggcg
    56341 attatgctgg cgcaagggga atacatcacc gggatcgatg acgacgatga atggataccc
    56401 aaccgcctga gcgtcttcct cgcccataaa cagcaactgg tcacacacgc ctttttgtac
    56461 gctaacgact acgtttgcca gggcgaagtt tattcccaac cggcaagctt gccgctgtat
    56521 cccaaatcgc cgtattcacg ccgcctgttt tacaaacgca atatcattgg caatcaggtc
    56581 tttacctggg cgtggcgttt caaagagtgc ttgttcgata ccaaactgaa agcggcacag
    56641 gattacgaca tcttcctgcg gatggtggtg gagtacggcg aaccgtggaa agtggaagag
    56701 gcgacgcaga tcctgcatat caatcatggt gaaatgcaga tcacctcgtc accgaaaaaa
    56761 ttctccggat acttccactt ttaccgcaag cacaaagaca aattcgaccg cgccagcaaa
    56821 aaatatcagc tgtttaccct ctaccagatc cgcaataaac gcatgacctg gcgcactttg
    56881 ctgacgctgc tgtcggtgcg taacggcaaa cgtctggctg atggtattcg ggggcgctaa
    56941 agatgctgga agatctgcgc gccaacagct ggagtttacg tccgtgctgc atggttcttg
    57001 cctatcgcgt cgctcatttt tgttcggtgt ggcgcaaaaa gaacgtcctc aacaatctgt
    57061 gggcggcccc gctgctggtg ctgtatcgca ttatcaccga atgctttttc ggttatgaaa
    57121 tccaggccgc cgcgaccata ggtcgacgct ttactatcca tcacggttac gccgtagtga
    57181 tcaataaaaa cgtggtcgcg ggggatgatt tcaccattcg ccacggcgtc actatcggca
    57241 atcgtggtgc cgataacatg gcatgtccac acattggcaa cggcgtcgaa ctcggtgcca
    57301 acgtcattat tcttggtgat atcacgcttg gtaacaacgt caccgtgggc gcgggcagcg
    57361 tggtgctcga ctctgtcccg gacaacgcgc tggtggtggg agaaaaagcg cgagtgaagg
    57421 taattaaatg aatattctgc aattcaatgt gcgactggcg gaaggcgggg cagcaggtgt
    57481 ggcgttagat ctccaccagc gtgcgctgca acaggggctg gcatcacatt ttgtctacgg
    57541 ctacggcaaa ggcggcaaag agagcgtcag tcatcaaaac tatccgcagg tcatcaaaca
    57601 tacgccgcgg atgaccgcga tggcgaacat tgcgttgttt cgtctgttta atcgcgatct
    57661 gttcggcaat ttcaatgagt tatatcgcac cattactcgc acaccgggtc cggtggtcct
    57721 gcattttcat gtgctgcaca gctactggtt gaatcttaag agcgtggtgc gcttttgcga
    57781 aaaagtgaaa aaccataaac cggatatcac tctggtctgg acgttacacg atcactggag
    57841 cgttaccgga cgctgcgcct ttaccgacgg ttgcgaaggc tggaaaacgg gctgccagaa
    57901 atgcccgacc ttaaataact atccaccggt gaagattgat cgcgcgcacc agctggtggc
    57961 gggtaaacgc cagttattcc gcgagatgct ggcactgggc tgtcagttta tttcccccag
    58021 ccagcatgtg gctgatgctt tcaatagtct gtacggtcca gggcgttgcc ggattatcaa
    58081 taacggtatt gatatggcaa ccgaagcgat tctggcggac ttgcctccgg tgcgcgaaac
    58141 ccaggacaag ccgaaaatcg cggtggtggc gcatgatctg cgttacgacg gcaaaactaa
    58201 ccagcaactg gtacgtgaga tgatggcgct gggcgacaaa attgaactgc atacctttgg
    58261 taagttctcg ccgttcaccg ctggcaacgt ggttaatcac ggctttgaaa ctgacaagcg
    58321 caagttgatg agcgcgctca atcagatgga tgcgctggta ttcagttctc gcgtcgataa
    58381 ctacccgctg attttgtgtg aggcgctatc gattggcgtg ccggtgattg ccacccatag
    58441 cgacgcggcg cgggaagtgc tgcaaaaatc cggcggtaaa accgtcagcg aagaagacgt
    58501 gctgcaactg gtgcagttaa gcaaaccgga aatcgcgcag gcgatatttg gtaccacgct
    58561 ggctgagttc agccaacgca gccgcgccgc ctacagtgga caacagatgc tggaggagta
    58621 tgtcaacttc tatcagaatc tgtagctacc tgctgctgcc gctgatttac ctgttggtta
    58681 atgtcaaaat cgcccagctt ggcgaaagtt tccccatcac catcgtcact tttttacctg
    58741 tcttgttact gctgttttta gaacgcatca gcgttaaaaa attgatgatt accttaggga
    58801 ttggcgcggg actcacggcg tttaactacc tgtttggtca gtcgttggat gccggtaaat
    58861 acgtcacttc aaccatgctg tttgtctata ttgtgatcat tattggcatg gtgtggagta
    58921 ttcgctttaa aacgatttcg ccacacaacc atcgcaagat attacgtttc ttttatctgg
    58981 tggtcgggct ggtggtggcg ctggcggcag tggagatggc gcaaattatc ctcaccggcg
    59041 gcagcagtat tatggagtcg atttcgaaat atctgattta cagcaacagc tatgtgctga
    59101 atttcatcaa attcggcggc aagcgcacga cagcacttta tttcgaaccg gcatttttcg
    59161 ctctggcatt aatctcaatt tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa
    59221 ccgatgctat gattctcgca gggataatat tatccggatc gttttcaggg gttatgacct
    59281 ttatcctgtt ttatttgctg gagtgggcat ttcaatatct gaataaagag gcgattaaga
    59341 aaaagttacc gttagcattg atttctctgg ctgtattcct ggttggtgtg gtaatcgcgt
    59401 ttccttatat ttccacccgt ctgggcgatt taggtacaga aggatcgtca tcgtattatc
    59461 gtattgtcgg tccgctggtg atggtcggtt attctttgac ccatattgac ggtgtagtca
    59521 gatttggctc actttatgaa tatgtcgcat cattcggaat atttaacggt gcggatgtcg
    59581 gaaaaaccat agacaatggt ttgtatctgc tgattattta tttttcctgg ttcgcagtgt
    59641 ttttatcgct gtggtacatg gggaaagtga taaaaatgat gatcaacgct tttggtgata
    59701 accgcaattt tcgcgtgcaa ttatatcttt ttactccggt atcgctgttt tttaccggtt
    59761 cgatatttag cccagaatat gcatttttaa tcgtctgtcc gtttattttg cgaaaagcgt
    59821 taaatattac gaggtaagaa taagaacatg ttgcttagca taatcactgt cgcgtttcgt
    59881 aacctcgaag ggatagtcaa aacacatgcc tcgctggcgc atctggcgca ggcggacgat
    59941 atcagcttcg aatggattgt tgtcgatggc ggttccaatg acggcacccg tgagtatctg
    60001 gaaaatctca atggtatcta taacctacgc tttgtcagcg agccagataa cggtatctac
    60061 gacgctatga acaaaggcat tgcgatggcg caaggcaagt tcgcgctgtt tctcaattca
    60121 ggcgatattt ttcatcagga tgccgcaaat tttgtccgta agttaaaagt gcaaaaagat
    60181 aacatgatga tcaccggcga tgcgctgctg gattttggcg acgggcataa gattaaacgt
    60241 agcgccaaac cggggtggta tatttatcac agcctgcccg ccagtcatca ggcgatattt
    60301 tttccagtat ccggcttgaa aaaatggcgt tatgacctgg aatataaagt ttcttccgac
    60361 tacgcgctgg cagccaaaat gtataaagcc ggttatgtat ttaaaaaact caatggcctg
    60421 gtgtctgaat tttccatggg tggggtatct accaccaata atatggaatt gtgtgctgac
    60481 gcgaaaaaag tccaacggca aatattacat gtacctggct tctgggcaga attatcctgg
    60541 catttacgcc agcgtactac ctcaaagacg aaagccttat ataacaaaag ctgaatataa
    60601 gggaaaacga tgcaggattt aagcggtttc tcggtgccga aagggttccg gggcagcaat
    60661 gctattaaag tgcaattgtg gtgggcggta caggcaacaa tatttgcctg gtcgccacaa
    60721 gtattgtatc gctggcgggc atttttatta cgtttattcg gcgcaaaaat aggaaaaaac
    60781 gtagttattc gtccgtcagt aaaaattacc tatccgtgga aattaacctt aggtgattac
    60841 gcgtgggtcg gcgatgaggt caatttatat accctcggtg aaataaccat tggcgcacat
    60901 tcggtgatat cgcaaaaaag ttatttatgc accgggagcc acgaccatgc aagtcaacat
    60961 ttcaccatta acgccacgcc tattgtgatt ggcgagaaat gctggctggc aaccgatgtc
    61021 tttgttgccc caggcgtcac gatcggcaac ggcaccgtcg tgggtgcgcg aagcagtgtt
    61081 tttaaatcgc ttccggcaaa tgtggtttgc cgggggaacc ccgcagtggt gatacgcaaa
    61141 cgcgttgaaa ctgaataaat tcaaaaaata cagaggaata agacatgtca aaagtcgctc
    61201 tcatcaccgg tgtaaccgga caagacggtt cttacctggc agagtttctg ctggaaaaag
    61261 gttacgaggt gcatggtatt aagcgtcgtg catcgtcatt caacaccgag cgcgtggatc
    61321 acatttatca ggatccgcac acctgcaacc cgaaattcca tctgcattat ggcgacctga
    61381 gtgatacctc caacctgacg cgcattttgc gtgaagtgca gccggatgaa gtgtacaacc
    61441 tgggcgcaat gagccacgtt gcggtttctt ttgagtcacc ggaatatacc gcagacgttg
    61501 atgcgatggg gacgctgcgc ctgctcgagg cgatccgctt cctcggtctg gaaaagaaaa
    61561 cccgtttcta tcaggcttct acttctgaac tgtacggtct ggtgcaggaa attccgcaga
    61621 aagagaccac gccgttctac ccgcgatctc cgtatgcggt tgccaaactg tacgcctact
    61681 ggataaccgt taactaccgc gaatcctacg gcatgtacgc ctgtaacggt attctcttca
    61741 accatgaatc cccgcgccgt ggcgagactt tcgttacccg caaaatcacc cgcgcaatcg
    61801 ccaacatcgc ccaggggctg gagtcgtgcc tgtatctcgg caatatggat tccctgcgtg
    61861 actggggcca tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa caggaacaac
    61921 cggaagattt cgttatcgcg accggcgttc agtactccgt acgtcagttc gtggaaatgg
    61981 cggcagcaca actgggcatc aaactgcgct ttgaaggcac gggtgttgaa gagaagggca
    62041 ttgtggtttc cgtcaccggg catgacgcgc cgggcgttaa accgggcgat gtgattatcg
    62101 ccgttgaccc gcgttacttc cgtccggcgg aagttgaaac gctgctcggc gacccgacca
    62161 aagcgcacga aaaactgggc tggaaaccgg aaatcaccct cagagagatg gtgtctgaaa
    62221 tggtggctaa tgacctcgaa gcggcgaaaa aacactctct gctgaaatct cacggctacg
    62281 acgtggcgat cgcgctggag tcataagcat gagtaaacaa cgcattttta tcgctggcca
    62341 tcgcggaatg gtcggttccg ccatcaggcg acagctcgaa cagcgcggtg atgtggagct
    62401 ggtattacgc acccgcgatg aactgaacct gctggacagc cgcgcggtgc atgatttctt
    62461 tgccagcgaa cgtattgacc aggtctatct ggcggcggcg aaagtgggcg gcattgttgc
    62521 caacaacacc tatccggcgg atttcatcta ccagaacatg atgattgaga gcaacatcat
    62581 tcacgccgcg catcagaacg acgtgaacaa actgctgttt ctcggatcgt cctgtatcta
    62641 cccgaaactg gcaaaacagc cgatggcaga aagcgagttg ttgcagggta cgctggagcc
    62701 gaccaacgag ccttatgcca ttgccaaaat cgccgggatc aaactgtgtg aatcttacaa
    62761 ccgccagtac ggacgggatt accgctcagt catgccaacc aacctgtacg ggccacacga
    62821 caacttccac ccgagtaatt cgcatgtgat cccagcattg ctgcgtcgct tccatgaggc
    62881 gacggcacag aatgcgccgg acgtggtggt gtggggcagc ggtacgccga tgcgcgaatt
    62941 tctacacgtt gatgatatgg cagcggcgag cattcatgtc atggagctgg cgcacgaagt
    63001 ctggcaggag aacacccggc cgatgctgtc gcacattaac gtcggcacgg gcgttgactg
    63061 cactatccgc gagctggcgc aaaccatcgc caaagtggtg ggttacaaag gtcgggtggt
    63121 ttttgatgcc agtaaaccgg atggcacacc gcgcaaactg ctggatgtga cgcgcctgca
    63181 tcagcttggc tggtatcacg aaatctcact ggaagcgggg cttgccagca cttaccagtg
    63241 gttccttgag aatcaagacc gctttcgggg gtaatgatgt ttttacgtca ggaagacttt
    63301 gccacggtag tgcgctccac tccgcttgtc tctctcgatt ttatagtcga gaacagtcgc
    63361 ggcgagtttc tgcttggcaa aagaaccaac cgcccggcgc agggttactg gtttgtgccg
    63421 ggagggcgcg tgcagaaaga cgaaacgctg gaggccgcat ttgagcggct gacgatggcg
    63481 gaacttgggt tgcgtctgcc gataacagca ggccagtttt acggtgtctg gcagcacttt
    63541 tatgacgata acttctccgg cacggatttc actactcact atgtggtgct cggttttcgc
    63601 ttcagagtcg cggaagaaga actgttactg ccggatgagc agcatgacga ttaccgctgg
    63661 ctgacgccgg acacgttgct tgccagtgac aatgttcacg ctaacagccg cgcctatttt
    63721 ctcgctgaga agcgtgccgg agtacccgga ttatgaaaat actggtctac ggcattaact
    63781 actcgccgga gttaaccggc atcggcaagt acaccggcga gatggtggaa tggctggcgg
    63841 cacaaggtca tgaggtgcgg gttattaccg caccgcctta ctacccgcaa tggcaggtgg
    63901 gcgagaacta ttccgcctgg cggtacaaac gggaagaggg ggccgccacg gtgtggcgct
    63961 gcccgctgta cgtgccaaaa cagccgagca ccctgaaacg cctgttgcat ctcggcagtt
    64021 ttgccgccag cagtttcttt ccgctgatgg cgcaacgtcg ctggaagccg gatcgcatta
    64081 tcggcgtggt gccaacgctg ttttgcacgc caggaatgcg cctgctggcg aaactgtctg
    64141 gtgcgcgcac ggtgctccat attcaggatt acgaagtgga cgccatgctg gggctgggcc
    64201 ttgccggaaa aggtaaaggc ggcaaagtgg cacagctggc aacggcgttc gaacgtagcg
    64261 gactgcataa cgtcgataac gtctccacga tttcgcgttc gatgatgaat agagctatcg
    64321 agaaaggcgt ggcggcggaa aacgtcatct tcttccccaa ctggtcagaa attgcccgtt
    64381 ttcagcatgt cgcagacgcc gatgttgatg cccttcgtaa ccagcttggc ctgccggata
    64441 acaaaaaaat cattctttac tccggcaata ttggtgaaaa gcaggggctg gaaaacgtta
    64501 ttgaagctgc cgatcgcctg cgcgatgaac cgcttatttt tgccattgtc gggcagggcg
    64561 gcggcaaagc gcggctggaa aaaatggcgc agcagcgtgg actgcgcaac atgcaatttt
    64621 ttccgctgca atcgtatgac gctttacccg cactgctgaa gatgggggat tgccatctgg
    64681 tggtgcaaaa acgcggcgcg gcagatgccg tattgccgtc gaaactgacc aatattctgg
    64741 cggtaggcgg taacgcggtg attaccgcag aagcccacac cgaactgggg cagctttgcg
    64801 aaacctttcc gggcattgcg gtttgcgtgg aaccggaatc ggtcgaggcg ctggtggcag
    64861 ggattcgtca ggcgctcctg ctgcccaaac acaacacggt ggcacgtgaa tatgccgaac
    64921 gcacgctcga taaagagaac gtgttacgtc aatttataaa tgatattcgg ggataattat
    64981 ggcgcagtcg aaactctatc cagttgtgat ggcaggtggc tccggtagcc gcttatggcc
    65041 gctttcccgc gtactttacc ccaagcagtt tttatgcctg aaaggcgatc tcaccatgct
    65101 gcaaaccacc atctgccgcc tgaatggcgt ggagtgcgaa agcccggtgg tgatttgcaa
    65161 tgagcagcac cgctttattg tcgcggaaca gctgcgtcaa ctgaacaaac tcaccgagaa
    65221 cattattctc gaaccggcag ggcgtaacac tgcaccggcc attgcgctgg cggcgctggc
    65281 ggcaaaacgt catagcccgg agaacgaccc attaatgctg gtgctggcgg cggatcatgt
    65341 gattgccgac gaagatgcgt tccgcgccgc cgtgcgtaac gccatgccgt atgctgaagc
    65401 gggcaagctg gtgaccttcg gcattgtgcc ggatctacca gaaaccggtt atggctatat
    65461 tcgtcgcggt gaagtgtcgg cgggtgagca ggatacggtg gcctttgaag tggcgcagtt
    65521 tgtcgaaaaa ccgaatctgg aaaccgcgca ggcctatgtg gcaagtggcg aatattactg
    65581 gaacagcggt atgttcctgt tccgtgccgg acgttatctc gaagaactga aaaagtatcg
    65641 tccggatatt ctcgatgcct gtgaaaaagc gatgagcgcc gtcgatccgg atctcgattt
    65701 tattcgtgtg gatgaagagg cgtttctcgc ctgtccggaa gagtcggtag attacgcggt
    65761 catggaacgc acggcagatg ccgttgtggt gccgatggat gcgggctgga gcgatgtcgg
    65821 ttcctggtcc tctttatggg agatcagtgc ccacaccgcc gagggcaacg tttgccatgg
    65881 cgatgtgatt aatcacaaaa ctgaaaacag ctatgtgtat gccgaatctg gcctggtcac
    65941 caccgtcggg gtgaaagatt tggtggtagt gcagaccaaa gatgcagtgc tgattgccga
    66001 ccgtaacgcg gtgcaggatg tgaaaaaagt ggtcgagcag atcaaagctg atggtcgcca
    66061 tgagcatcgg gtgcatcgcg aagtgtatcg tccgtggggc aaatatgact ctatcgacgc
    66121 gggcgaccgc taccaggtga aacgcatcac cgtgaaaccg ggcgaaggct tgtcggtaca
    66181 gatgcaccat caccgcgcgg aacactgggt ggttgtcgcg ggaacggcaa aagtcactat
    66241 cgacggtgat atcaaactgc ttggtgaaaa cgagtccatt tatattccgc tgggggcgac
    66301 gcattgcctg gaaaacccgg ggataattcc gctcgattta attgaagtgc gctccggctc
    66361 ttatctcgaa gaggatgatg tggtgcgctt cgcggatcgc tacggacggg tgtaaacgtc
    66421 gcatcaggcg ctgtttgtcg gatgcggcgt gaacgcctta tccgacctac ggttcgggtt
    66481 tgtaggcctg ataagacgcg gcagcgtcgc atcaggcaat gaatgcgaaa ccgcgatgta
    66541 aataacgaca aaaacaaaat tggccgtttc ggtcagggcc aactattgcc tgaaaaaggg
    66601 taacgatatg aaaaaattaa cctgctttaa agcctatgat attcgcggga aattaggcga
    66661 agaactgaat gaagatattg cctggcgcat tggtcgcgcc tatggcgaat ttctcaaacc
    66721 gaaaaccatt gtgttaggcg gtgatgtccg cctcaccagc gaaaccttaa aactggcgct
    66781 ggcgaaaggt ttacaggatg cgggcgtcga tgtgctggat attggcatgt ccggcaccga
    66841 agagatttat ttcgccacgt tccatctcgg cgtggatggc ggcatcgaag ttactgccag
    66901 ccataacccg atggattata acggcatgaa gctggtgcgc gagggggctc gcccgatcag
    66961 cggcgatacc ggactgcgcg atgtccagcg tctggcagaa gccaacgact ttccccccgt
    67021 cgatgaaacc aaacgcggtc gctatcagca aatcaacctg cgtgatgcat acgttgatca
    67081 cctgttcggt tatatcaacg tcaaaaacct cacgccgctc aagctggtga tcaactccgg
    67141 gaatggcgca gcgggtccgg tggtggacgc cattgaagcc cgctttaaag ccctcggcgc
    67201 accagtggaa ttgatcaaag tgcacaacac gccggacggc aatttcccca acggaattcc
    67261 taacccgctg ctgccggaat gccgcgacga cacccgcaat gcggtcatca aacacggcgc
    67321 ggatatgggt attgcctttg atggcgattt tgaccgctgc ttcctgtttg acgaaaaagg
    67381 gcagtttatt gaaggctact acattgtcgg tttgctggca gaagcgttcc tcgaaaaaaa
    67441 tcccggcgcg aagatcatcc acgatccgcg tctctcctgg aacaccgttg atgtggtgac
    67501 tgccgcaggt ggcacgccgg taatgtcgaa aaccggacac gcctttatta aagaacgtat
    67561 gcgcaaggaa gacgccatct acggtggcga aatgagcgct caccattact tccgtgattt
    67621 cgcttactgc gacagcggca tgatcccgtg gctgctggtc gccgaactgg tgtgcctgaa
    67681 agggaaaacg ctgggcgaac tggtgcgcga ccggatggct gcgtttccgg caagcggtga
    67741 gatcaacagc aaactggcgc accccgttga ggcgattaac cgcgtcgaac agcattttag
    67801 ccgcgaggca ctggcggtgg atcgtaccga tggcatcagc atgacctttg ccgactggcg
    67861 ctttaacctg cgctcctcca acaccgagcc ggtggtgcgg ttgaatgtgg aatcacgcgg
    67921 tgatgtgccg ctgatggaag cgcgaacgcg aactctgctg acgttgctga acgagtaatg
    67981 tcggatcttc ccttacccca cttgcgggta aggggctaaa aacaggaaca acgatgacaa
    68041 atctaaaaaa gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct
    68101 tttcagatat caccatcatg tttgccggac tatggctggt ttgcgaagtc agcggactgt
    68161 cattcctcta catgcacctg ttggtggcgc tgattacgct ggtggtgttc cagatgctgg
    68221 gcggcatcac cgatttttat cgctcatggc gcggtgttcg ggcagcgaca gaatttgccc
    68281 tgttgctaca aaactggacg ttaagcgtga ttttcagcgc cggactggtg gcgttcaaca
    68341 atgatttcga cacgcaattg aaaatctggc tggcgtggta tgggctgacc agcatcggac
    68401 tggtggtttg ccgttcatgt attcgtattg gggcgggctg gctgcgtaat catggctata
    68461 acaagcgtat ggtcgcggtg gcgggggatt tagccgccgg acaaatgctg atggagagct
    68521 tccgtaatca gccgtggtta gggtttgaag tggtgggcgt ttaccacgac ccgaaaccgg
    68581 gcggcgtttc taacgactgg gcgggtaatc tgcaacagct ggtcgaggat gcgaaagcgg
    68641 gcaagattca taacgtctat atcgcgatgc agatgtgcga cggcgcgcga gtgaaaaaac
    68701 tggtccatca actggcggac accacctgtt cggtgctgct gatccccgat gtctttacct
    68761 tcaacattct ccattcccgt atcgaagaga tgaacggcgt accggtggtg ccgctgtatg
    68821 acacgccgct ttccggggtt aaccgcctgc tcaaacgtgc ggaagatatt gtgctggcga
    68881 cgcttattct gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg gtgaaactga
    68941 gctcaccggg gccggttatt ttccgccaga ctcgctacgg catggatggc aagccgatca
    69001 aagtgtggaa gttccgttcc atgaaagtga tggagaacga caaagtggtg actcaggcga
    69061 cgcagaacga tccgcgcgtc accaaagtgg ggaactttct gcggcgcact tcgctggatg
    69121 aactgccgca gtttatcaat gtgctgactg gcgggatgtc gattgtgggt ccccgtccgc
    69181 atgcggtggc gcataacgaa cagtatcgcc agctcattga aggctacatg ctgcgccata
    69241 aagtgaaacc gggcattacc ggctgggcgc agattaacgg ctggcgcggc gaaaccgaca
    69301 cgctggagaa aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg
    69361 tctggttcga tatcaaaatc gttttcctga cggtattcaa gggcttcgtt aacaaagcgg
    69421 catattaata tgagcttacg tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgcc
    69481 acggtgatca tcatcggcct cggtctggtg caaatgactg tgctggcgcg gattatagat
    69541 aaccaccagt tcggtttgct gactgtgtcg ctggtgatca tcgcgctggc agatacgctt
    69601 tctgacttcg gtatcgctaa ctcgattatt cagcgaaaag aaatcagcca ccttgaactc
    69661 accacgctgt actggctgaa cgttgggctg gggcttgtgg tgtgtgtggc ggtgtttttg
    69721 ttgagtgatc tcatcggcga cttgctgaat aacccggacc tggcaccgtt gattaaaaca
    69781 ttgtcgctgg cgtttgtggt gatcccccac gggcaacagt tccgcgcgtt gatgcaaaaa
    69841 gagctggagt tcaacaaaat cggcatgatc gaaaccagcg cggtgctggc gggctttact
    69901 tttacggtgg ttagcgccca tttctggccg ctggcgatga ccgcgattct tggttatctg
    69961 gtcaatagtg cggtgagaac gctgctgttt ggctactttg gccgcaaaat ttatcgcccc
    70021 ggtctgcatt tctcgctggc gtcggtggca ccgaacttac gctttggtgc ctggctgacg
    70081 gcggacggca tcatcaacta tctcaatacc aacctttcaa cgctggtgct ggcgcgtatt
    70141 ctcggcgcgg gcgtggctgg gggatacaac ctggcgtaca acgtggccgt tgtgccgccg
    70201 atgaaactga acccgatcat cacccgcgtg ttgtttccgg cgttcgccaa aattcaggac
    70261 gatacagaaa agctgcgcgt caacttctac aagctgctgt cggtggtggg gattatcaac
    70321 tttccggcgc tgctggggct gatggtggtg tcgaataact ttgtaccgct ggtctttggt
    70381 gagaagtgga acagcattat tccggtgctg caattgctgt gtgtggtggg gctgctgcgt
    70441 tccgtgggta acccgattgg ttcgctgctg atggcgaaag cgcgggtcga tatcagcttt
    70501 aaattcaacg tattcaaaac ctttctgttt attccggcga ttgttattgg tgggcagatg
    70561 gcgggcgcaa tcggcgtcac gcttggcttc ctgctggtgc agattatcaa caccattctg
    70621 agctatttcg tgatgattaa accggtgctc ggttccagtt atcgccagta catcctgagt
    70681 ttgtggctgc cgttttatct ctcgctgccg acgctggtgg ttagttctgc gctaggcatt
    70741 gtgctgaaag ggcaactggc gctggggatg ctgctggcgg tgcaaatagc cgcaggcgtg
    70801 ctggcgtttg tggtgatgat tgtgctgtcg cgccattcgc tggtggtgga agtgaagcgt
    70861 cagttttgtc gcagcgaaaa aatgaaaatg cttttacggg cggggtgaat ggttgttttc
    70921 ggttccggca acggttgccg gatgcggcgt gaacgcctta tccgtcctgg ggggctgggc
    70981 ttggatcttg taggcctgat aagacgcgtt agcgtagcat caggcactga atgccggatg
    71041 cggcgtaaaa cgccttatcc ggcctacggt tctgtgctcg gatcttgtag gcctgataag
    71101 gcgcgtcagc gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc ttatccggcc
    71161 tacggttctg tgctcggatc ttgtaggcct gataaggcgc gtcagcgtcg catcaggcac
    71221 tgaatgccgg atgcggcgta aaacgcctta tccgtcctac ggttctgtgc tcggatcttg
    71281 taggcctgat aagacgcgtc agcgtcgcat caggcactga atgccggatg cggcgtgaac
    71341 gccttatccg gcctacggtt ctgtgctcgg gacttgtagg tctgataaga cgcgttagcg
    71401 tcgcatcagg caaaaaacgg caacgaacac aacaaacata gcctcacctg gcatcaaatt
    71461 tatgaggaaa aaatgaaatt acttattctg ggcaaccaca cttgcggcaa tcgtggcgac
    71521 agcgccatct tgcgcggctt acttgatgcc atcaatatcc tcaatccaca tgccgaagtg
    71581 gacgtgatga gccgctatcc ggtcagttct tcctggctgc tcaaccgccc ggtaatgggc
    71641 gatccgctgt tcctgcaaat gaaacaacac aacagcgcag cgggcgtggt tgggcgcgtt
    71701 aaaaaagtcc tccgtcgccg ctaccagcac caggtattgc tctcacgcgt caccgacact
    71761 ggcaagttgc gtaatatcgc catcgcccag ggattcactg acttcgtgcg actgctgtca
    71821 ggttacgacg ccattattca ggtcggcgga tcgttttttg tcgatctcta tggcgtgccg
    71881 cagtttgaac atgcgctttg cacgtttatg gcgaaaaagc cgctgtttat gattggtcac
    71941 agcgtcggcc cgttccagga tgagcaattt aaccaactgg cgaactacgt ttttggtcac
    72001 tgcgacgcgc tgatcctgcg cgaatcggtc agccttgaac tgatgaaacg cagcaatatc
    72061 accaccgcaa aagttgaaca tggcgtcgat accgcgtggc tggtcgatca ccacaccgaa
    72121 gacttcaccg ccagctatgc cgttcaacac tggctggacg ttgccgcaca acagaaaacg
    72181 gtggccatta ccctgcgcga actggcaccg ttcgacaaac gtctcggcac cactcaacaa
    72241 gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc tcgatgaagg gtatcaggtg
    72301 attgcgctct ccacctgtac gggcatcgac agctataaca aagacgaccg catggtggcg
    72361 ctcaatctgc gccagcacat cagcgatcct gcccgttacc acgtggtgat ggatgaactc
    72421 aatgatctgg aaatgggcaa aattctgggg gcctgtgaac tcaccgtcgg tacgcgcctg
    72481 cactccgcca ttatctcaat gaactttgcc accccggcga ttgccatcaa ctatgaacat
    72541 aaatccgccg ggatcatgca acaactgggg ctaccagaaa tggcgattga tatccgtcat
    72601 ttattagacg gcagcctgca agcgatggtt gcggatacct taggccagct tccggcactg
    72661 aacacgcgac ttaacgaagc cgtcagtcgc gaacgtcaga cgggaatgca gatggtgcaa
    72721 tctgtgcttg aacgcatcgg ggaggtgaaa tgaaggtcgg cttcttttta ctgaaatttc
    72781 cgctgtcgtc ggaaaccttc gttcttaacc aaatcactgc gtttattgat atgggctttg
    72841 aggtggagat tgtcgcgttg caaaaaggcg atacagagaa cacccacgcg gcatggacga
    72901 aatacaacct tgccgccaga acccgctggt tacaggacga acctacgggc aaagtggcga
    72961 agctgcgcca ccgcgccagc cagaccttac gcggcattca tcgtaaaaat acctggcagg
    73021 cgctcaacct caaacgctat ggtgccgagt cgcggaacct gattttgtct gccatttgtg
    73081 gccaggtcgc aacaccgttt catgccgatg tctttatcgc tcattttggc ccggcggggg
    73141 taaccgcagc aaaactacgc gaactgggtg tcattcgcgg caaaattgcc actatcttcc
    73201 acggtattga tatctccagt cgggacgttc tcaaccacta cactcccgaa tatcagcaac
    73261 tgttccgccg tggcgacctg atgctaccga tcagcgatct ctgggccgga aggctgcaaa
    73321 aaatgggctg tccgagggaa aaaatcgccg tatcgcgcat gggcgtggac atgacgcgct
    73381 ttagcccgcg tccggtgaaa gcgcccgcaa cgccgctgga aatcatttcc gtcgcacgct
    73441 taaccgaaaa aaaaggcctg catgtggcga tcgaagcctg ccgtcagttg aaagagcagg
    73501 gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg ggaacgacgc ctgcgcaccc
    73561 tcatcgaaca atatcaactg gaagatgtgg tggagatgcc gggctttaaa ccgagccatg
    73621 aagtgaaagc gatgctagac gacgcggatg tcttcctgtt gccatcggtt acaggtgcgg
    73681 atggcgatat ggaaggtatt ccggtggcgc tgatggaagc gatggcggtc ggcattccgg
    73741 tggtttctac tctgcatagc ggaatacctg aactggtgga ggccgataaa tccggctggc
    73801 tggtgcctga gaacgatgct cgcgcactgg cgcaacgact ggcggcgttt agccaactgg
    73861 acagcgacga actggctcct gtcgtcaaac gtgcgcgcga aaaagtcgaa cacgatttta
    73921 accagcaggt gatcaatcga gaactcgcca gcttgctgca ggctttatag aggagaacgc
    73981 atgccattta aaaaactctc ccgacgcacc ttcctgacgg caagctcggc gcttgccttc
    74041 ctccataccc ctttcgctcg cgcacttccc gctcaacaaa gcgttaacat taacgactac
    74101 aaccctcacg actggatcgc ctcatttaaa caagccttca gcgaagggca aacggtcgtc
    74161 gtgcccgccg gattgatttg tgacaatatc aacaccggca tcttcattcc tcctggcaaa
    74221 acgttacaca tccttggaag cctgcgcggc aacggcagag ggcgatttgt cttacaggat
    74281 ggcagccagg tgacagggga ggggggcggc agtatgcata acatcaccct ggatgtgcgc
    74341 ggctctgact gcaccatcaa agggctggcg atgagcggct ttggtccggt aacgcagatt
    74401 tatatcggcg gcaaaaacaa acgggtcatg cacaacctga ccatcgataa cctgaccgtt
    74461 agccacgcta attatgccat cttacgccag ggatttcata accagattat cggtgccaac
    74521 atcaccaact gtaagttcag cgacttacag ggggacgcta ttgaatggaa cgtggcgatt
    74581 aacgacagtg atattttgat atctgaccat gtcatcgagc gcatcaactg tactaacggc
    74641 aaaatcaact ggggcatcgg cataggcctt gcgggaagta cctacgataa taattatccg
    74701 gaaaaccagg cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcaga ttgtcggcaa
    74761 ctgatccatg ttgaaaatgg taaacatttt gttattcgta atatcaaagc ccgcaatatc
    74821 acaccggatt tcagtaagaa agcaggtatt gataacgcca cagttgctat ttatggttgt
    74881 gacaactttg tgattgataa tattgaaatg attaatagtg ccgggatgtt aatcggctat
    74941 ggggtaatta aaggcaaata tctctcgata ccgcagaatt tccgagtgaa tgatattcaa
    75001 ctggataata ctcatcttgc ttataaattg cgcggcatcc aaatctccgc cgggaatgct
    75061 gtctcctttg tggcgctgac taacattgag atgaagcctg cgtcgcttga gctacacaat
    75121 aaaccgcaac acctttttat gagaaatatc aatgtgatgc aggaatcctc tgttggaccc
    75181 gcattgagca tgaacttcga catgcgcaaa gacgtacgcg gtgtctttat ggcgaaaaaa
    75241 gaaacactgc tgtcacttgc aaatgttcat gcgatgaatg aaaaaggaca aagctccgtc
    75301 gatatcgaca ggattaatca ccatattgtt aatgtggaaa agattaactt tagattgccg
    75361 gaacggagag agtagatttg cgaccattcc tggaaaaatg gagccatact taggaacaat
    75421 gctactgcaa tccacaacga agcggcgtaa catcacaagt aattcagtaa tcaattcagg
    75481 gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg
    75541 gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg
    75601 ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa gccaatgatt
    75661 cagtacatcg ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcac
    75721 gcttccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga atctctcctt
    75781 gaacagcgcg tgaagcgtca actgcttgcg gaagtgcagt ccatctgtcc accgggcgtg
    75841 accattatga acgtgcgtca gggcgaacct ttaggtttgg gccactccat tttatgtgca
    75901 cgacctgcta ttggtgacaa cccatttgtc gtggtactgc cagacgttgt tatcgatgac
    75961 gccagcgccg acccgctgcg ttacaacctt gctgccatga ttgctcgttt caacgaaacg
    76021 ggccgcagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata ctccgttatt
    76081 cagaccaaag aaccactgga tcgtgaaggt aaagtcagcc gcattgttga atttatcgaa
    76141 aaaccggatc agccgcagac gctggactca gacatcatgg ccgtgggccg ttatgtgctt
    76201 tctgccgata tttggccgga actggaacgt actcagcctg gtgcatgggg acgtattcag
    76261 ctgactgatg ccattgctga gttggcaaaa aaacaagcag ttgacgcaat gctgatgact
    76321 ggggacagtt acgactgcgg aaagaaaatg ggttatatgc aagcgtttgt gaagtatggg
    76381 ctgcgtaacc tgaaggaggg cgctaagttc cgcaaaggca ttgagaagtt gttaagcgag
    76441 taacaaattc ctgactggaa gtgtcggtca gtaagtaatt aaaaacggca gtgaagattc
    76501 atagcataag ttttgctcat tgaatctcac tgccgttgtc ttttttataa gtacaataaa
    76561 aacagcatgt tagcttgcaa tcatagttca aatttttcca ggattttcct tgtttctaca
    76621 gctgtttggt aagacaatta gcgtttgaat ttttcggatt tggcacgagt gggttacgct
    76681 cgtcacatcg tggaaatgta cgcagtgctc tggtagctgt taagccaggg gcggtagcgt
    76741 gcattaatgc atctattaat caaatctgga gcagtctatt tcacagcatg ctctctggct
    76801 atatggaata aaaaagtgaa gatacttgtt actggtggcg caggatttat tggttctgct
    76861 gttgttcgtc acataataaa taatacgcaa gatagtgttg ttaatgtcga taaattaaca
    76921 tacgccggaa acctggaatc gctcgctgaa atttctgatt ctgaacgtta ttcatttgag
    76981 catgcagata tctgcgatgc cgaagcgatg gctcgtattt tcgcacagca ccagccagac
    77041 gcggtgatgc acctggcagc agagagccac gttgaccgct caataactgg ccctgcggca
    77101 tttattgaaa ccaatattgt gggtacttat gttcttttag aagcggcgcg caattattgg
    77161 tctggtctgg atgatgaaaa gaaaaaaaac ttccgctttc atcatatttc tactgatgag
    77221 gtgtatggtg acttacccca tccggatgaa gtaaatagca atgaaacgtt gccgctattt
    77281 acggaaatga cagcatacgc gccaagtagt ccatattctg cttctaaagc ttccagcgat
    77341 catttggttc gcgcatggaa acgtacttat ggtttaccga ccattgtgac taattgctcg
    77401 aacaactatg gtccttatca tttcccggaa aagcttattc cactggttat tcttaatgca
    77461 ctggaaggta aggcattacc tatttatggc aaaggagatc agatccgcga ctggttgtat
    77521 gtagaggatc atgctcgagc gttatatacc gtcgtaaccg aaggtaaagc gggcgaaact
    77581 tataacattg gtggacacaa cgaaaagaaa aacatcgacg tagtgttcac tatttgtgat
    77641 ttgttggatg agatagtccc gaaagagaaa tcttatcgtg agcaaattac ctatgttgct
    77701 gatcgcccag ggcatgatcg ccgttatgca attgatgccg ataaaattag ccgcgaattg
    77761 ggctggaaac cacaggaaac gtttgagagc gggattcgta aaactgtgga atggtatctg
    77821 tccaatacaa aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag
    77881 aactatgagg gccgccacta atgaatatcc tcctttttgg caaaacaggg caggttggtt
    77941 gggaactaca gcgtgctctg gcacctctgg gtaatttgat tgctcttgat gttcactcca
    78001 ctgattactg tggtgatttt agtaaccctg aaggtgtggc tgaaaccgtt agaagcattc
    78061 ggcctgatat tattgtcaac gcagccgctc acaccgcagt agacaaagca gaatcagaac
    78121 cggagtttgc acaattactg aacgcgacga gtgtcgaagc gatcgcgaaa gcagccaatg
    78181 aagtcggcgc ttgggttatt cactactcta ctgactacgt atttccgggg accggtgaaa
    78241 taccatggca ggaggaggat gcaaccgcac cgctaaatgt ttacggtgaa accaagttag
    78301 caggagaaaa agcattacaa gagcattgtg cgaagcacct tattttccgg accagctggg
    78361 tctatgcagg taaaggaaat aacttcgcca aaacgatgtt gcgtctggca aaagagcgtg
    78421 aagaattagc cgttattaat gatcagtttg gtgcgccaac tggcgcagag ttgctggctg
    78481 attgtacggc acatgccatt cgtgtggcac tgaataaacc ggaagtcgca ggtttgtacc
    78541 atctggtagc cagtggtacc acaacctggc acgattatgc tgcgctggtt tttgaagagg
    78601 cgcgcaaagc aggcattccc cttgcactca acaagctcaa cgcagtacca acaacagtct
    78661 atcctacacc agctcgtcgt ccacataact ctcgccttaa tacagaaaaa tttcagcaga
    78721 actttgcgct tgtcttgcct gactggcagg ttggtgtgaa acgcatgctc aacgaattat
    78781 ttacgactac agcaatttaa tagtttttgc atcttgttcg tgatggtgga acaagatgaa
    78841 ttaaaaggaa tgatggaatg aatacgcgta aaggtattat tttagcgggt ggttctggta
    78901 cacgtcttta tcctgtgact atggctgtca gtaaacagct gttaccgatt tatgataaac
    78961 cgatgatcta ttacccgctc tctacactga tgttggcggg tattcgcgat attttgatta
    79021 tcagcacgcc acaggatact cctcgttttc aacaactgct gggtgatggg agccagtggg
    79081 ggctaaatct tcactacaaa gtgcaaccga gtccggatgg tcttgcgcag gcatttatca
    79141 tcggtgaaga gtttatcggt ggtgatgatt gtgctttggt acttggtgat aatatcttct
    79201 acggtcacga cctgcctaag ttaatggatg ccgctgttaa caaagaaagt ggtgcaacgg
    79261 tatttgccta tcacgttaat gatcctgaac gctatggtgt cgttgagttt gataaaaacg
    79321 gtactgcaat cagcctggaa gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg
    79381 ggctttattt ctatgataac tacgttgtgg aaatggcgaa aaatcttaag ccttctgccc
    79441 gcggtgaact ggaaattacc gatattaacc gtatctatat ggaacagggg catttatctg
    79501 ttgccatgat gggacgtgga tatgcctggc tggacacggg gacacatcaa agtcttattg
    79561 aagcaagcaa cttcattgcc accattgaag agcgccaggg cttgaaagtt tcctgcccgg
    79621 aagaaattgc ttaccgtaaa gggtttattg atgctgagca ggtgaaagta ttagctaaac
    79681 cgctgaaaaa aaatgcttat ggtcagtatc tgctaaaaat gattaaaggt tattaataaa
    79741 atgaatgtta ttaaaacaga aattccagat gtactgattt ttgaaccgaa agtttttggt
    79801 gatgagcgtg gtttctttat ggaaagcttt aatcagaaag ttttcgaaga ggctgtaggg
    79861 cggaaggttg aatttgttca ggataatcat tctaaatcgt gtaaaggtgt acttagaggt
    79921 ttacactttc agcttcctcc ctttgagcag gcaaaattag taaggtgtat agttggcgag
    79981 gtatttgatg ttgcagtaga cattagacct aattctgaaa catttggttc atgggttgga
    80041 gtaactcttt cgtcagaaaa taaaaggcag ctatggattc cagaaggatt cgcccatggt
    80101 tttttaactt taagtgatat tgcagagttt gtttataaaa ctaacaacta ttattcttta
    80161 aatcatgaaa ggggagtcat ttggaacgat gaggaaatta acattgcctg gccctctcaa
    80221 tcagagaaga ttctgtcaca gaaagatatt aatttaccat catttag
//
LOCUS       DAZFRE010000010        80012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFRE010000010 DAZFRE010000000
VERSION     DAZFRE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.010"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            35..586
                     /locus_tag="ACHKUI_000797"
     CDS             35..586
                     /locus_tag="ACHKUI_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFO6597056.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(764..1756)
                     /gene="rpoS"
                     /locus_tag="ACHKUI_000798"
     CDS             complement(764..1756)
                     /gene="rpoS"
                     /locus_tag="ACHKUI_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6597057.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1819..2913)
                     /gene="nlpD"
                     /locus_tag="ACHKUI_000799"
     CDS             complement(1819..2913)
                     /gene="nlpD"
                     /locus_tag="ACHKUI_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6597058.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3098..3724)
                     /gene="pcm"
                     /locus_tag="ACHKUI_000800"
     CDS             complement(3098..3724)
                     /gene="pcm"
                     /locus_tag="ACHKUI_000800"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6597059.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3718..4479)
                     /gene="surE"
                     /locus_tag="ACHKUI_000801"
     CDS             complement(3718..4479)
                     /gene="surE"
                     /locus_tag="ACHKUI_000801"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6597060.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4460..5509)
                     /gene="truD"
                     /locus_tag="ACHKUI_000802"
     CDS             complement(4460..5509)
                     /gene="truD"
                     /locus_tag="ACHKUI_000802"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6597061.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKVLMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5506..5985)
                     /gene="ispF"
                     /locus_tag="ACHKUI_000803"
     CDS             complement(5506..5985)
                     /gene="ispF"
                     /locus_tag="ACHKUI_000803"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6597062.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5985..6695)
                     /gene="ispD"
                     /locus_tag="ACHKUI_000804"
     CDS             complement(5985..6695)
                     /gene="ispD"
                     /locus_tag="ACHKUI_000804"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6597063.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLVNHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6714..7025)
                     /gene="ftsB"
                     /locus_tag="ACHKUI_000805"
     CDS             complement(6714..7025)
                     /gene="ftsB"
                     /locus_tag="ACHKUI_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6597064.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7218..7541)
                     /gene="ygbE"
                     /locus_tag="ACHKUI_000806"
     CDS             complement(7218..7541)
                     /gene="ygbE"
                     /locus_tag="ACHKUI_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6597065.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7591..8196)
                     /gene="cysC"
                     /locus_tag="ACHKUI_000807"
     CDS             complement(7591..8196)
                     /gene="cysC"
                     /locus_tag="ACHKUI_000807"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6597066.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8196..9623)
                     /gene="cysN"
                     /locus_tag="ACHKUI_000808"
     CDS             complement(8196..9623)
                     /gene="cysN"
                     /locus_tag="ACHKUI_000808"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6597067.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9625..10533)
                     /gene="cysD"
                     /locus_tag="ACHKUI_000809"
     CDS             complement(9625..10533)
                     /gene="cysD"
                     /locus_tag="ACHKUI_000809"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6597068.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10785..11822
                     /gene="iap"
                     /locus_tag="ACHKUI_000810"
     CDS             10785..11822
                     /gene="iap"
                     /locus_tag="ACHKUI_000810"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6597069.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11947..12099)
                     /locus_tag="ACHKUI_000811"
     CDS             complement(11947..12099)
                     /locus_tag="ACHKUI_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6597070.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12364..13098)
                     /gene="cysH"
                     /locus_tag="ACHKUI_000812"
     CDS             complement(12364..13098)
                     /gene="cysH"
                     /locus_tag="ACHKUI_000812"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6597071.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNVQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13172..14884)
                     /gene="cysI"
                     /locus_tag="ACHKUI_000813"
     CDS             complement(13172..14884)
                     /gene="cysI"
                     /locus_tag="ACHKUI_000813"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6597072.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14884..16683)
                     /gene="cysJ"
                     /locus_tag="ACHKUI_000814"
     CDS             complement(14884..16683)
                     /gene="cysJ"
                     /locus_tag="ACHKUI_000814"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6597073.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMVKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            17002..17364
                     /gene="queD"
                     /locus_tag="ACHKUI_000815"
     CDS             17002..17364
                     /gene="queD"
                     /locus_tag="ACHKUI_000815"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6597074.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            17442..18713
                     /gene="ygcN"
                     /locus_tag="ACHKUI_000816"
     CDS             17442..18713
                     /gene="ygcN"
                     /locus_tag="ACHKUI_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6597075.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCTRAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18704..18964
                     /gene="ygcO"
                     /locus_tag="ACHKUI_000817"
     CDS             18704..18964
                     /gene="ygcO"
                     /locus_tag="ACHKUI_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6597076.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18981..19556
                     /gene="ygcP"
                     /locus_tag="ACHKUI_000818"
     CDS             18981..19556
                     /gene="ygcP"
                     /locus_tag="ACHKUI_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6597077.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19704..20564)
                     /gene="ygcQ"
                     /locus_tag="ACHKUI_000819"
     CDS             complement(19704..20564)
                     /gene="ygcQ"
                     /locus_tag="ACHKUI_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6597078.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLTEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20561..21340)
                     /gene="ygcR"
                     /locus_tag="ACHKUI_000820"
     CDS             complement(20561..21340)
                     /gene="ygcR"
                     /locus_tag="ACHKUI_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6597079.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVVLETAADLRFASE
                     FVARHIAEWQRQNPLDLIIIGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQNYLRQRMQP"
     gene            complement(21318..22727)
                     /gene="ygcS"
                     /locus_tag="ACHKUI_000821"
     CDS             complement(21318..22727)
                     /gene="ygcS"
                     /locus_tag="ACHKUI_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6597080.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22749..24203)
                     /gene="ygcU"
                     /locus_tag="ACHKUI_000822"
     CDS             complement(22749..24203)
                     /gene="ygcU"
                     /locus_tag="ACHKUI_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6597081.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24273..25058)
                     /gene="ygcW"
                     /locus_tag="ACHKUI_000823"
     CDS             complement(24273..25058)
                     /gene="ygcW"
                     /locus_tag="ACHKUI_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6597082.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            25376..26653
                     /gene="yqcE"
                     /locus_tag="ACHKUI_000824"
                     /pseudo
     CDS             25376..26653
                     /gene="yqcE"
                     /locus_tag="ACHKUI_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
     gene            26680..28158
                     /gene="ygcE"
                     /locus_tag="ACHKUI_000825"
     CDS             26680..28158
                     /gene="ygcE"
                     /locus_tag="ACHKUI_000825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6597083.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSEN
                     AAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            28323..29243
                     /locus_tag="ACHKUI_000826"
     CDS             28323..29243
                     /locus_tag="ACHKUI_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6597084.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGE
                     SAFIGKVGDDPFGRFMYQTLSKENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPTFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            29411..30928
                     /locus_tag="ACHKUI_000827"
     CDS             29411..30928
                     /locus_tag="ACHKUI_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6597085.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30989..32359
                     /locus_tag="ACHKUI_000828"
     CDS             30989..32359
                     /locus_tag="ACHKUI_000828"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO6597086.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            32359..33768
                     /locus_tag="ACHKUI_000829"
     CDS             32359..33768
                     /locus_tag="ACHKUI_000829"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6597087.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDFFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQQGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            33794..34801
                     /locus_tag="ACHKUI_000830"
     CDS             33794..34801
                     /locus_tag="ACHKUI_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6597088.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34886..35557)
                     /gene="queE"
                     /locus_tag="ACHKUI_000831"
     CDS             complement(34886..35557)
                     /gene="queE"
                     /locus_tag="ACHKUI_000831"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6597089.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            35730..36332
                     /locus_tag="ACHKUI_000832"
     CDS             35730..36332
                     /locus_tag="ACHKUI_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO6597090.1"
                     /translation="MLRIFIAIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQTWQQAQAQV
                     SRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNTNAH"
     gene            36346..37017
                     /locus_tag="ACHKUI_000833"
                     /pseudo
     CDS             36346..37017
                     /locus_tag="ACHKUI_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            37269..38417
                     /locus_tag="ACHKUI_000834"
     CDS             37269..38417
                     /locus_tag="ACHKUI_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO6597091.1"
                     /translation="MRIFLLLLMLYGFQALAEQLPSVTDLTGTLTTEEQTALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQSYGLYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLKEMLPPEQAAQIVHYHMQPEFKKNNLFSGLTQGIEGIAQFTYIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFTSRPGPGIWMISMITPMVWFFFFQ
                     DDVIIRRVSLVCFSLFFAATCWQRIAVIFHMYRVFPMMLAPKNKNAKVKKQKTTQQVS
                     QSERNSLFVVLLIIVVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGHKSNRKTGNRNAHSRSSSKNSFRGGGGSSGGGGSSDRW"
     gene            38423..39295
                     /locus_tag="ACHKUI_000835"
     CDS             38423..39295
                     /locus_tag="ACHKUI_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6597092.1"
                     /translation="MRIILFLLTLWCLPVLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITQKTRAQVAVLIVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(39355..40653)
                     /gene="eno"
                     /locus_tag="ACHKUI_000836"
     CDS             complement(39355..40653)
                     /gene="eno"
                     /locus_tag="ACHKUI_000836"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6597093.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40741..42378)
                     /gene="pyrG"
                     /locus_tag="ACHKUI_000837"
     CDS             complement(40741..42378)
                     /gene="pyrG"
                     /locus_tag="ACHKUI_000837"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6597094.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(42606..43397)
                     /gene="mazG"
                     /locus_tag="ACHKUI_000838"
     CDS             complement(42606..43397)
                     /gene="mazG"
                     /locus_tag="ACHKUI_000838"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6597095.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(43469..43804)
                     /gene="mazF"
                     /locus_tag="ACHKUI_000839"
     CDS             complement(43469..43804)
                     /gene="mazF"
                     /locus_tag="ACHKUI_000839"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6597096.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(43804..44052)
                     /gene="mazE"
                     /locus_tag="ACHKUI_000840"
     CDS             complement(43804..44052)
                     /gene="mazE"
                     /locus_tag="ACHKUI_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6597097.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(44130..46364)
                     /gene="relA"
                     /locus_tag="ACHKUI_000841"
     CDS             complement(44130..46364)
                     /gene="relA"
                     /locus_tag="ACHKUI_000841"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6597098.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRDSVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(46412..47713)
                     /gene="rlmD"
                     /locus_tag="ACHKUI_000842"
     CDS             complement(46412..47713)
                     /gene="rlmD"
                     /locus_tag="ACHKUI_000842"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6597099.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            47770..50526
                     /gene="barA"
                     /locus_tag="ACHKUI_000843"
     CDS             47770..50526
                     /gene="barA"
                     /locus_tag="ACHKUI_000843"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6597100.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRNGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(50757..52097)
                     /gene="gudD"
                     /locus_tag="ACHKUI_000844"
     CDS             complement(50757..52097)
                     /gene="gudD"
                     /locus_tag="ACHKUI_000844"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO6597101.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(52118..53458)
                     /gene="gudX"
                     /locus_tag="ACHKUI_000845"
     CDS             complement(52118..53458)
                     /gene="gudX"
                     /locus_tag="ACHKUI_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO6597102.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQAHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(53460..54812)
                     /gene="gudP"
                     /locus_tag="ACHKUI_000846"
     CDS             complement(53460..54812)
                     /gene="gudP"
                     /locus_tag="ACHKUI_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6597103.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(55246..55695)
                     /gene="yqcA"
                     /locus_tag="ACHKUI_000847"
     CDS             complement(55246..55695)
                     /gene="yqcA"
                     /locus_tag="ACHKUI_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6597104.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(55713..56495)
                     /gene="truC"
                     /locus_tag="ACHKUI_000848"
     CDS             complement(55713..56495)
                     /gene="truC"
                     /locus_tag="ACHKUI_000848"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6597105.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWCGLLPENERVEFSAPSGQDGEISS"
     gene            complement(56495..56824)
                     /gene="yqcC"
                     /locus_tag="ACHKUI_000849"
     CDS             complement(56495..56824)
                     /gene="yqcC"
                     /locus_tag="ACHKUI_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6597106.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDYPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(57446..57991)
                     /gene="syd"
                     /locus_tag="ACHKUI_000850"
     CDS             complement(57446..57991)
                     /gene="syd"
                     /locus_tag="ACHKUI_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6597107.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            58059..58907
                     /gene="queF"
                     /locus_tag="ACHKUI_000851"
     CDS             58059..58907
                     /gene="queF"
                     /locus_tag="ACHKUI_000851"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6597108.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            59019..60383
                     /gene="ppnN"
                     /locus_tag="ACHKUI_000852"
     CDS             59019..60383
                     /gene="ppnN"
                     /locus_tag="ACHKUI_000852"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6597109.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEKFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            60940..62229
                     /gene="sdaC"
                     /locus_tag="ACHKUI_000853"
     CDS             60940..62229
                     /gene="sdaC"
                     /locus_tag="ACHKUI_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6597110.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            62287..63654
                     /gene="sdaB"
                     /locus_tag="ACHKUI_000854"
     CDS             62287..63654
                     /gene="sdaB"
                     /locus_tag="ACHKUI_000854"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6597111.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            63766..64521
                     /gene="xni"
                     /locus_tag="ACHKUI_000855"
     CDS             63766..64521
                     /gene="xni"
                     /locus_tag="ACHKUI_000855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6597112.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(64625..65773)
                     /gene="fucO"
                     /locus_tag="ACHKUI_000856"
     CDS             complement(64625..65773)
                     /gene="fucO"
                     /locus_tag="ACHKUI_000856"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6597113.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDVAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(65801..66448)
                     /gene="fucA"
                     /locus_tag="ACHKUI_000857"
     CDS             complement(65801..66448)
                     /gene="fucA"
                     /locus_tag="ACHKUI_000857"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6597114.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            66995..68311
                     /gene="fucP"
                     /locus_tag="ACHKUI_000858"
     CDS             66995..68311
                     /gene="fucP"
                     /locus_tag="ACHKUI_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6597115.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            68344..70119
                     /gene="fucI"
                     /locus_tag="ACHKUI_000859"
     CDS             68344..70119
                     /gene="fucI"
                     /locus_tag="ACHKUI_000859"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6597116.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEALLNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            70228..71646
                     /gene="fucK"
                     /locus_tag="ACHKUI_000860"
     CDS             70228..71646
                     /gene="fucK"
                     /locus_tag="ACHKUI_000860"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6597117.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            71648..72070
                     /gene="fucU"
                     /locus_tag="ACHKUI_000861"
     CDS             71648..72070
                     /gene="fucU"
                     /locus_tag="ACHKUI_000861"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6597118.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            72128..72859
                     /gene="fucR"
                     /locus_tag="ACHKUI_000862"
     CDS             72128..72859
                     /gene="fucR"
                     /locus_tag="ACHKUI_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6597119.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(72903..74003)
                     /gene="rlmM"
                     /locus_tag="ACHKUI_000863"
     CDS             complement(72903..74003)
                     /gene="rlmM"
                     /locus_tag="ACHKUI_000863"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6597120.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(73996..74391)
                     /gene="ygdD"
                     /locus_tag="ACHKUI_000864"
     CDS             complement(73996..74391)
                     /gene="ygdD"
                     /locus_tag="ACHKUI_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6597121.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(74410..75327)
                     /gene="gcvA"
                     /locus_tag="ACHKUI_000865"
     CDS             complement(74410..75327)
                     /gene="gcvA"
                     /locus_tag="ACHKUI_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6597122.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(75678..75905)
                     /gene="ygdI"
                     /locus_tag="ACHKUI_000866"
     CDS             complement(75678..75905)
                     /gene="ygdI"
                     /locus_tag="ACHKUI_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6597123.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            76097..77302
                     /gene="csdA"
                     /locus_tag="ACHKUI_000867"
     CDS             76097..77302
                     /gene="csdA"
                     /locus_tag="ACHKUI_000867"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6597124.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            77302..77745
                     /gene="csdE"
                     /locus_tag="ACHKUI_000868"
     CDS             77302..77745
                     /gene="csdE"
                     /locus_tag="ACHKUI_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6597125.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TTAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(77796..78602)
                     /gene="tcdA"
                     /locus_tag="ACHKUI_000869"
     CDS             complement(77796..78602)
                     /gene="tcdA"
                     /locus_tag="ACHKUI_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6597126.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     MVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(78679..79776)
                     /gene="mltA"
                     /locus_tag="ACHKUI_000870"
     CDS             complement(78679..79776)
                     /gene="mltA"
                     /locus_tag="ACHKUI_000870"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6597127.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
BASE COUNT        19564 a        20932 c        19543 g        19973 t
ORIGIN      
        1 caaaaaatca ttcattagta aaaaggatat ttatatgcaa aacatagctg tggtttatta
       61 ttcaggatat ggtcacacta aactggttgc tgaaaaagtg gcacaaggcg cccatgcaga
      121 tttaatttcg attgataacg aaggaaatat cactgagcag gagtgggaaa aactggacgc
      181 agccgatgcc attatcttcg gtgctcccac ctatatgggc ggtgcgccat ggcaattcaa
      241 gaaatttgct gatgccagtt ccaaagcgtg gtttacacgt aaatggcagg ataaagtctt
      301 cgggggtttc accaacagcg ccagcctgaa tggcgataaa caagttacgc tgatctatct
      361 gcaaacgctg gcttcacagc atggcggtct gtgggtgagt ttaggtcagg ctcctgccaa
      421 tgtgcttgcg tcaaccaggg aagatgtaaa caatctgggc ggttcagtcg gtttgctggt
      481 gcaatctcca gccgatgcgg gtgcagacca aatccctacg ggcgatctgg atacagccgt
      541 gaaatatggt gaacgcgtgg cgacaattac tgcgcgctta aaatgatgat tgccgaatgt
      601 gacgctgacg cgtctatccg gtcttctaca tctaaaaagt gccgtcccct ggtcagtcag
      661 ataaagtttt aacgctgtat ccgataacag ttcaacatgt accatgtacc aaaagaaaaa
      721 aagccagcct cgcttgagac tggcctttct gacagatgct tacttattcg cggaacagcg
      781 cttcgatatt cagcccctgc gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct
      841 gaatctggcg aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg
      901 ccgcttcgta ccccagcaaa ccgaatcgac gtgccagcac ttcacgctgt ttggcgttca
      961 gctcgaacag ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg
     1021 gaccattctc tttttcatcg gccaggatgt ccagcaacgc tttttcggaa tcaccaccca
     1081 gcggggtgtc taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa
     1141 ctggcttatc cagttgctct gcgatctctt ccgcacttgg ttcgtggtcc agcttatggg
     1201 acaactcacg tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac
     1261 gaatagtacg ggtttggttc ataatcgccc gttcgatcgt ctggcgaatc caccaggttg
     1321 cgtatgttga gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc
     1381 ccaggttgcc ctcttcgatc aggtccagca acgccagacc acgattgcca taacggcggg
     1441 caatttttac caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc
     1501 cacgcagtgc gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac
     1561 caatctcacc aaggtaaagc tgagtcgcgt ccagcacacg ctgtgtggct ccctgcgata
     1621 acagttcctc ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt
     1681 caaaaacctc aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca
     1741 gcgtattctg actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa
     1801 aagcctggtt ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc
     1861 cttgtaacga atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc
     1921 tattttttgc cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc
     1981 gtaggcactc aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag
     2041 cgcgttaccg gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct
     2101 gcctgcgata tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt
     2161 gccctcagtc ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt
     2221 cggctcggtt gtgcttgctg ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg
     2281 cttgttgttc ggcaacattt tgttagcact ctgttcaccc gaagactcag aatacgtaat
     2341 tgtcggttgc gacgcaacag caacggtgga attttgtgca ggcttgatca caactccttg
     2401 ctctgctgcg tcggcctggg taatggcatt tccgcctgtg attggcgtac cggaagcatt
     2461 acccacctgt aaggtctgac cgacgttcag cgcgtatggt gcctgaatat tgttgcgctg
     2521 agcaaggtca cggaaatcgt tgccagtaat ccaggcgata taaaaaagcg tgtcgccttt
     2581 tttcacggtg tatgtactgc cgctataact gcctttcgga atgttcccat actgacggtt
     2641 gtagacgatt cgtccgtttt ccatctgtac cggctgctga gctactggct gcacaggctg
     2701 gatttgcggt tgttgagtag cctgaatttg tggctgctgt accggctgaa tttgcggttg
     2761 ctgcgctgta gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattggt
     2821 atttgcgggc gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga
     2881 acagcctgcc agccatagcg aaaccagtga caatgccgca atgcggcgaa cggtgaattt
     2941 tgggcttccc gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt
     3001 accgtgcaag gcaccatact gaacactgga aaagatgttc acgatacgct gacttgcggc
     3061 aaaataacca ggaaaaatcc aggtatttcc tcacgtttta agccagctca cccttcacta
     3121 aagggacaaa gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca
     3181 cccgttttaa atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca
     3241 gctgcgtcat tagcgcagtt ggaatttcag gcggtgccgc cgtaacaatg atagcgtcaa
     3301 acggcgcgcg tgcctgccaa ccttgccatc catcgccatg acgggttgaa acattatgta
     3361 aatcaagatt tttcaggcgg cgacgcgcct gccactgcaa gcctttaatc cgttcaaccg
     3421 agcaaacatg ttggacaaga tgcgccagga ttgcagtttg atatcccgaa ccagtgccaa
     3481 tttccagcac ccgcgactgc ggcgtcagct cgagtaattc ggtcattcgc gccaccatat
     3541 acggctgcga aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt
     3601 gttcaaacgc ttcatcaacg aatttttcac gcggcacggc ggcaagtgca ttcagcacca
     3661 gctcatcctg aataccttgc gcacgtaatt gatccagaag tgcttgtacg cgtctgctta
     3721 ccattgcgtg ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg
     3781 cgcagttaaa tccacatgca gcggcgtgat ggaaacatag ccctcatcta ccgcagcaaa
     3841 atcggtcccc ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt
     3901 gccgcgcgga tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt
     3961 cacgcgaata cctttgattt gatccaaggg taaatccgga acgttaatat taagaatacg
     4021 cccggtgcgc agcggctctt tacacagtgc gcgcaaaatt gagcaggtta ccgccgcggc
     4081 agtgtcgtaa tgtttatgcc cgtcaagcga gacggcaagc gccggaaaac ctaaatgacg
     4141 gccttccatc gcggccgcta ccgtaccgga ataaataaca tcatccccca gattcggccc
     4201 ggcgttaatt ccggacacaa caatgtccgg gcgcgggcgc atcagagcat ttacgccaag
     4261 atagacgcaa tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa
     4321 cgtgcgcagg gaggattcca gtgtcagaga attagaagcg ccgctgcggt tacgatcggg
     4381 ggcgaccacc tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg
     4441 tgcatgtacc ccgtcatcat tactcagcaa tatgcgcata atcacctgtt gtgttgataa
     4501 gttccctgac aacgctggtt gcaaaacttc ccgccggaag ccagaaacgg atttctacgg
     4561 tgacatcatc ccaccagttc cagcttaatt gttgcggata cagcagcatc gctctgcgcg
     4621 cggcttcaac tttttcgcgc accagtaagg tttgtaattc agtttcttcg gcgacagctg
     4681 cttgttcgaa tgccagcgct tcacgctgag ttccccactc gccactgccc ggcaatgcgg
     4741 cggttatcat cagcacttta tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg
     4801 ttgcgacaaa ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat
     4861 taacgtctgc ttttttgagg cgttcagcaa caatctgatt aaacaacgca ctgcgggctg
     4921 ccgacaacca aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc
     4981 gcagcgcacc ctgcaagttg ctaccgccaa tcccaaaacg ttgggcacca aagtagttcg
     5041 gtacaccttt tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt
     5101 cgcgtagtac cagggtaaag gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt
     5161 gccgcgcata ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcatct
     5221 ctttgcccgg cacgcgagcg cataaccact gttccgtaac agcatgttta tctttttgcc
     5281 cagcgaagct gacttcacgg gcatgaattt tcaggaattt cgccagtgca tccgccacaa
     5341 aacgggtatt gcagccgttt ttgaggattc taaccagaat atgctcacct tcaccatcag
     5401 gctcaaagcc caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg
     5461 tgccttgcgg tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt
     5521 aatgagtagc gccaccgctt cacaggcaat cccttcccca cgtccggtaa atcccagttt
     5581 ttccgtagta gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat
     5641 aaacacgcgc atttgtggaa tgtgcggcaa catcttcggt gcctgagcga tgatagtgac
     5701 atcgacgttg ccgagggcat aacctttcgc ctgaatacga cgccaggctt cgcgcaacag
     5761 ctcgcggctg tcggcacctt taaatgccgg atcggtatcc gggaacagct tgcctatatc
     5821 tcccagcgcc gccgcgccaa gcaatgcatc ggtcaacgca tggagcgcca cgtcgccatc
     5881 agaatgcgcc agcagccctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc
     5941 ttcaccgcca aaggcgtgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga
     6001 tggatggttc gggtgaggta aaattcggcc agcgccaaat cctccgggcg cgttaccttg
     6061 atgttatccg cgcggccttc aacaagttgc ggatgaaaac cgcaatattc cagcgccgag
     6121 gcctcatcgg taatggtagc gccttcattt agagcgcgcg tcaggcagtc atgtaacagc
     6181 tcacgaggga aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga
     6241 gcaatggcat ttttgcccgg ttcggcacgt ttcatcgtat cgcgcaccgg cgcagcgagg
     6301 atccctcccg tgcggctggt ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc
     6361 agacaaggac gagcggcgtc atgcaccaat acccactgcg cgtcgccagc ggctttcaga
     6421 cctgccagca cggaatcggc acgctcttca ccaccatcaa caaccgtgat tcgtggatga
     6481 ttcaccagag gaagttgtgc aaagcggcta tcgccaggac ttatggcaat gacgacacgt
     6541 ttcaccctgg gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg
     6601 attgagagat attgcttagg acattccgtt tgcattcggc ggccaaatcc ggccgccgga
     6661 accacggcgc aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga
     6721 ttgttttgcc ccgcagactg tgcgcgcttt gacgcgtcag gcaccagacg ataaaacgtt
     6781 tcgcccggcc tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg
     6841 ccattgagat cgtcaatttc ggcaaaaagc tgatcgtttc gcgctttaag tttcgcgttt
     6901 gtagcttgct gtgccgccac atcattattg acgcgagtat agtcatgtat accgttctta
     6961 ccgaaccaca gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta
     7021 cccatcctgc cccctgaaaa acggcatctt catcccatgc atccgaagac gactctacat
     7081 cctctgttgg ggataccgcg acaacgcggg caaatgtacc acatttgtcc attgttacgt
     7141 atacccaggg cgtgcagaac ataatctcat tattagttac ggattgaatt atgaacagaa
     7201 tagagaggaa agaacaatta acccagtagc cacataaaca gcgcaccaaa cataatgcca
     7261 accgtcgcca gagtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagt
     7321 gcaatcccca ccaccactgc gacgggcatc agcgccagaa agaaaggcca ggtgtagata
     7381 aaaaagagca gcgtgttaga gccataaatc aacatcggaa ttgccaacgc gcacagccac
     7441 gataaaaaac caaccacagc gcctggcagc gaccatgtgg tgtcttcaac ctcagtaagg
     7501 ctgtcgttat ttgttaatgt aatgttgtgg ctgttacgca tatctgatcc tgttactttg
     7561 acgaaccggg catggaaacc cggcggtgtc tcaggatctg ataatatcgt tctgtctcaa
     7621 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
     7681 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
     7741 acgcgctttc ttatataaac cttttggatc gcgggcttcg caaatcgcca gcggcgtatc
     7801 gacaaacact tcgataaagc gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc
     7861 gcggtgtggc gagataaatg cggtcagcac caccagtccg gcttcaacca tcaaattcgc
     7921 cacttcaccg acgcgacgga tattctcttt acggtcggca tcgctaaaac cgagatcgct
     7981 gcataatccg tggcgaacgt tgtcgccatc cagcagatac gtactgacgc caagtttatg
     8041 taacgcctcc tccagcgccc ccgcgacggt tgatttaccg gaaccagaga ggccggtaaa
     8101 ccacagcaca acaccacgat gaccgtggtg ctgttcgcgt tgttgcggag ttactgggtg
     8161 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatctcccag taaatcgcgc
     8221 gcaccccaat gcgggaaatg gcggcgaacc agggcattca attccagttc gaatgcactg
     8281 aattcagatg gcgcagcagt tgcctggcta actggttcgt gcaccatacc ggcacctacg
     8341 gtcacattac tcaggcgatc gataaaaatc agcccaccgg taaccgggtt ttgctgataa
     8401 cgatctaaca ccagtggctc gtcaaaggtg agatctacca ggccgatgcc gttcagcggc
     8461 aggttttcaa cttcgcgttg ggtaaggtta ttgatatcaa cctgataacg aatgccatca
     8521 acacgagcac gcgtcttctt accggcaatt ttgatgtcgt aactctggcc cggggaaagc
     8581 ggctgttccg ccatccatac cacatccacc gacgcgctct gcacagccgg taacgcttcg
     8641 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
     8701 atcgcttctc cggcaaaggc ttcttcgcga tcgccatcaa aggtcacgat ccgcgcgacg
     8761 tttgattcca caccagaggg cagcactttg acacgttgcc cgacttccac gcgaccggat
     8821 gccagcgttc cggcgtagcc acgaaaatcg agattcggac ggttaacgta ctgcaccggg
     8881 aagcgcattg gctgagcatc caccactcgc tggatctcca cggtttccag cacttctagc
     8941 agtgtcggac cgctgtacca cggcatactt tcactttgcg aagccacgtt gtcgccttcc
     9001 agtgcggaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaagtc
     9061 agataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
     9121 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
     9181 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
     9241 gctccagtcg ccatattgcg ggtgtactgc tcgtgccctg gggtgtcggc gataataaat
     9301 ttacgcttct cggtagagaa atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc
     9361 tcagcttgca ggccgtccac cagcagtgcc agatccagct tttcgccctg ggtgccgtga
     9421 cgcttactgt cgttatgcag cgatgagagc tgatcttcgt agatttggcg ggtatcgtgt
     9481 agcaggcgac caatcagggt acttttgccg tcatcgacac taccacaggt cagaaaacgc
     9541 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
     9601 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
     9661 ccccgcctgg tcgcggtcaa ttacgcggcc ctgacgttca ctggtggtgg aaaccagcat
     9721 ctcttcgatg atctccggca gtgtttgcgc atttgactcc accgcgccgg tcagcggcca
     9781 acagcccagt gtgcggaaac gcaccatccg ttttttaatc acttcgcccg gttgcaggtc
     9841 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
     9901 agcaagatac agcggaacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
     9961 ctcggtccag ttagagagcg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
    10021 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
    10081 gaaagagtag attcgctctt tagcgcggga tttctcttcg tcacggcgcg caccaccgaa
    10141 ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg ccttcagttt tcataatatc
    10201 ggtatgtttc gcgctgccgt gtacgaatgg attaatcccc atcgccacgc cttccgggtt
    10261 tttatgcacc agcagctcgc agccgtaggc tttcgccgta cgatcgcgga actcatacat
    10321 ctcgcggaat ttccagccgg tatcgacatg cagcaacggg aaaggcagcg tacctggata
    10381 aaacgccttg cgcgccagat gcagcatgac gctggaatct ttaccgatag agtagagcat
    10441 caccggattt gagaattctg cagccacctc gcgaataatg tggatgcttt ccgcctccag
    10501 ttgccgcagg tgggtaagtc gtttttgatc cataaccgtt cctttgcaat accgctattt
    10561 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
    10621 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aagtgcttaa
    10681 ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt ttagcgcagc ggcagtttca
    10741 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
    10801 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
    10861 cacctaaacc tggcgatttt gctaataccc aggcacgaca tattgctact ttctttccgg
    10921 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgt caacagtttc
    10981 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tataccgccc
    11041 gcgataatcg caagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
    11101 gcaaagcgcc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
    11161 atgctgacgc cgatgccaat ctcggcgggc taacgttaca aggaatggat gataacgccg
    11221 caggtttagg tgtcatgctg gaactggctg aacgcctgaa aaatacgcct accgagtatg
    11281 gtattcgatt tgtggcgacc agcggcgaag aggaaggaaa attaggcgct gagaatttac
    11341 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
    11401 taattgttgg cgataagctg tatttcaaca gtggcgtaaa aacacctgaa gcagtaagga
    11461 aattaacgcg cgaccgggcg ctggcaattg cccgcagcca tggaattgcc gccaccacaa
    11521 atccaggttt gaataaaaac tatccgaaag gcaccgggtg ttgtaatgac gcagaaatat
    11581 tcgacaaagc cggtattgct gtactttcgg tggaagcgac taactggaat cttggcaata
    11641 aggatggtta tcagcaacgc gcaaaaacag ccgcattccc tgcgggaaat agctggcatg
    11701 acgtaagact ggataatcaa cagcatattg ataaggcact tcctggaaga atagaacgtc
    11761 gctgccgtga tgttatgcgg ataatgctac cgttggtgaa ggagctggcg aaggcatctt
    11821 aatgggtttg aaatgattac tcccttgcct gcaggaaatc aatgccacca gaaacagagt
    11881 ataaaaaagg gtgcaggaga ccaaaccatc acaattcagt acgcggggtt ttaccccgcg
    11941 tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac cacgttctct
    12001 gatactcaac tcacacaacg agtcgcctac catcaacgtg aatcccagta ctgtacaaca
    12061 cagtacgatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa
    12121 gagactaaca tccgaattgc ttaggttcga acgtaggcct caggttgata gaaatatcgc
    12181 ctggggcttt tgtccatctg gaacctcgcg aatgcttaac gccagacagc ctcaagcacc
    12241 caatgccatt ctatacctgt taattctccc tgcgacgttc tcgtttaaat gctggaatgc
    12301 tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag gcgtttaatt tatccggcaa
    12361 tatttaccct tcgtgtaatc cacattccct ttttaagcca aagaaacgcg tttcttcttc
    12421 cgccatgccg ggttcccatt tacgggttgt atgggtatcg cccaccgata aatatccttc
    12481 atcccataat gggtgatatt tcaggccatg tttttgcagg tactgataaa tagttcggtt
    12541 atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca gcaccggtaa
    12601 attggcgcgg ctgccggatt gttcacggcg caggccagca aaccaggttt gcacattcag
    12661 ttctttcagc gcccggttca tcggttcgac tttgttgatg tcattgtact tttcaatgcc
    12721 ttcaacgccc tgctcccaca gtttgccgta gcgtgcttcc tgccaggcgg cgctttcggt
    12781 agcacggtac actttcaggt tgagcttgag tttatccgtt aactcgtcaa taaagcggta
    12841 ggtttccggg aacagatagc cggtatcggt gaggatcact ggaatatccg ggcgaatctg
    12901 attcaccaga tgcagactca ccgccgcctg aatgccaaag ctggaagaaa gtacatattc
    12961 accgggcaga ttatccagcg cccaggctac gcggccttca gcgtccagtt tttccagttg
    13021 ggcgttagtt tccgccagcg ccagaatccg gtctaccttt ggcagttcgt tcagggcgtt
    13081 tagatcgagt ttggacataa gttcctcact gtttgccttg cctgatgcaa cgcttacaca
    13141 tcttatcagg ccaacaagac cgggctgatg gttaatccca caaatcgcgc gccggatcga
    13201 gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc gaagccttca cccgcttcgc
    13261 gctctttcgc ccagcgccct atcagttcat caagcgacgc caggatttcc ggctcggtga
    13321 tgttttcttt atacatccgt gggatacgtg tcccaatgcg gttgccgcca agatgcaggt
    13381 tgtagcgacc cggcgctttc cccaccagac ccacttccgc cagcatcgcg cgaccacaac
    13441 cgttcgggca gcctgttaca cgcatcacga tatgctcatc gctgacacca tgtttcgcca
    13501 ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg ttccgcttcc gccatcgcca
    13561 gcgggcaagt cgggaatgac acgcaggcca ttgagttttc acgctgcggc gtgacggcat
    13621 tcattaaccc gctttctttg gcgatcttct cgatcttcgc tttctcgctt tccggcactc
    13681 cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc acctttgtgg atcttcgcga
    13741 tctccagcaa gccggttttc agcggacgtc ccggataatc aagaatgcga ccattttcga
    13801 taaacagcgt caggtgccag ttatcatcaa tgcccttaac ccagccaata cgatcgccgc
    13861 gtccggtaaa ctcatacggg cgaatcggct cgaatttgat ccccgcgcga cgttcaactt
    13921 ccgctttaaa cgtctcaacc cctacgcgct ccagcgtgta tttggttttg gcatttttac
    13981 gatcggttcg gttaccccag tcacgctgag ttgtcacgac ggcttccgcc accgccagcg
    14041 tatgctccag cggcagatag ccaaactcac tcgccgtgcg ggcgtaggtt ttcttgttgc
    14101 cgtgttcaat ggaaagccca ccgcccacca gcaggttaaa gcccaccagc ttgccgtttt
    14161 cggcgatcgc cacgaagttc atgtcgttgg cgtgcagatc gatatcgttc tgcggcggga
    14221 tcactaccgt ggttttgaac ttacgcggca ggtaggtctg accaaggatc ggctcttcat
    14281 cagtagtggc gactttttcc tgatcgagcc agatctccgc atacgcgcgg gtacgaggca
    14341 gcagatgttc agagattttc ttcgcccatt catatgcttc cgcgtgcagt tgcgactcgt
    14401 aagggttcga ggtgcagagt acgttacggt tcatgtcgtt ggcggtcgcc agcgcatcaa
    14461 gaccgactga gtgcagcatc tgatgcaccg gtttgacgtt ctttttcaga atgccgtgga
    14521 actggaatgt ctggcggttg gtcaggcgaa tgctgccata gatggtgttt tcaccggcaa
    14581 atttgtcgat cgcctgccac tgtttagtgg taatcacccc acccggcaga cgacagcgaa
    14641 gcagcatcgc gtggcgcggc tccagcttct gttcagcacg ttcggcgcga atgtcgcggt
    14701 catcctgctg atacatgccg tggaagcgga tcagcaggaa gttatcgccc ttaaaaccgc
    14761 ctgtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg ctttcaagct
    14821 tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa aggccctgga tgtttttcgc
    14881 tcattagtag acatctcgct gataacggcg ctctacgcgc agctcactta aaaattcatc
    14941 cgccgcttcg gtgtccatgc caccaaattc ggcaatcact tccagaagtg cctgctcaac
    15001 gtctttcacc atgcgattag cgtcgccgca gacataaatg tgggcaccgt cattgatcca
    15061 gcgccacagc tctgcgccct gttcgcgcag tttgtcttgt acgtaaattt tttctttttg
    15121 atcgcgcgac caggcgagat cgatgcgcgt cagcacgcct tctttgacgt aacgctgcca
    15181 ttccacctgg tagaggaaat cttccgtaaa gtgtgggttg ccaaagaata gccagttttt
    15241 acccggcgct tcgtcggcag cacgctgctg cataaaggca cggaacggcg cgatgccggt
    15301 gccagggcca atcatgatca ccggggtttc cggattggtt ggcaggcgga agttgtcgtt
    15361 atgttcgata aagacgcgga cttcaccctc ttcttccaca cggtccgcga ggaagctgga
    15421 ggcaccaccg gcacgggcac ggccttccac gtcgtaacgc accacaccaa cggtaacgtg
    15481 cacttcgttc tcgacttccg cctgcgagga ggcgatggaa tacaggcgcg gggtcagcgg
    15541 gcgcagcaga ttaatcagcg cttcggcatc cagttgcgcc ggagagaaac gcaccatgtc
    15601 gacaatcggc gtcgtcgcgg cgtaatgctg taatttcgct ttatcgccca ccagcggcag
    15661 cagcgtttcg ctgcgggtaa gcgtggcata gttctcaaca atgttggcgg tgttgacggt
    15721 cagttcgaag tgccactgta gcgcttcgtt cagaggcaac gttttgccct cgacggtgac
    15781 aggttcatcg cctttcagcc acagcagttc gacaagttct ttcaccagtg ccggatcgtt
    15841 ctgataccag acgcccagcg cgtcacccgg ctggtaacgc aggccagagt cacctaagtc
    15901 aatttcaatg tgacgaacgt ctttttcaga gttacgcccg gtaattttct ggttaacaga
    15961 gaggctcgcc accagtggcg cgtctttgct atacgggctg gtgtggattt catttaccgt
    16021 gccagtagcg acggattgcg aaggtgccgc gacaggcgca cgcgatttaa gcgcatcaac
    16081 cacgcgggcg cgccactcgc tggcagcagc ctggtattca acgtcggcat cgacacggtc
    16141 gagcaggcgt tcaccgccca gttccgccag cttactgtca aaatctttcc cggactggca
    16201 gaaaaattca taagagctat cgccgaggct aaacacggca aacgcggtgt tttccagctt
    16261 tggcgctttt ttggagaaca ggaacttatg cagcgcgacg gcttcttccg gcggttcccc
    16321 ttccccttgc gttgacgtca ccacgataag cagtttttcg ctggcgattt gtttgaattt
    16381 atagtcaccc gcgttcacca gcttaacgtt cagttttgcc gctaataaat catcgcgtaa
    16441 tgcttcagca acccggcgcg cattgccggt ttgcgaggcg gagataatag ttatacccgg
    16501 catttctgcg gcgggcgctg gcgtcgctgc aagcgcagca ggctgctggt tgagcacgcc
    16561 ccagaaatag ccagaaaccc aggcaagctg ggtaggagtt aaatcggtcg tggccgcctg
    16621 aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg gaaggtggga cctgtgtcgt
    16681 catgcgttgt tatgttccag taagcaaagc tgtttctgcg ccctgtcagc gcccataaaa
    16741 cagaagagaa ggtaaggtta acggggtaaa cggtgtggat taaagacggg atagcgataa
    16801 ctaataacca aatcgactaa cctgttttag caatagtctt taacaacaaa atagattaac
    16861 caacccaatg aaaaacaaat gaatattgcc catcaataag ataaatcagc gattttgcgc
    16921 aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc ggtactctac ggcggtttta
    16981 tcgtcatgta gagaaattat gatgtccacc acgttattta aagatttcac cttcgaagcc
    17041 gctcaccgct tgccgcacgt cccacaaggg cataaatgtg gtcgcctgca cgggcattcc
    17101 tttatggtgc gactggaaat taccggggaa gtcgatccgc atacgggctg gattatcgat
    17161 ttcgctgaac tgaaagcggc gtttaaacca acttatgagc gcctcgatca ccattatctc
    17221 aatgatattc caggtctgga aaacccaacc agcgaagttt tagcaaaatg gatttgggat
    17281 caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac ctgcaccgca
    17341 ggttgtatct atcgcggcga atgataagag tgtgtcggcg gtcaatttcc cttaagtaac
    17401 gctatgttag ggtgttgtgt tctggatatc tggggcatga catggaagac gactgcgaca
    17461 ttattattat cggtgccggt attgctggca ccgcttgcgc gttacgctgc acgcgagcgg
    17521 gtttatccgt tttgttactg gaacgagcgg agattcccgg cagcaaaaat ctttccggcg
    17581 ggcggctata cacccatgcg ctcgcggaac tcctcccgca atttcacctg accgcgcctc
    17641 ttgaacgacg catcactcac gaaagtcttt ctctgttaac gccagatggc gcaacgacgt
    17701 tttccagctt actgcccggc ggtgaatcct ggagtgtatt acgcgcacga ttcgatccgt
    17761 ggctggttgc cgaagccgaa aaagaaggtg tcgaatgcat ccccggtgcg acggtagatg
    17821 cactgtatga agaaaacggc agggtgtgtg gtgtcatttg tggtgacgat attcttcgcg
    17881 cccgttatgt ggtgctggca gaaggtgcca acagcgtcct ggcggaacgc cacgggttag
    17941 tgactcgtcc tgctggcgaa gcgatggcat tggggatcaa agaagtgctg acgctggaac
    18001 catccgctat tgaagaacgt tttcatctgg agaataacga aggcgcagcg ttgctgttca
    18061 gcggcggaat ctgtgatgac ttacccggcg gcgcatttct ttatactaat caacaaacgc
    18121 tctcgttagg gattgtttgc ccgctctcct cccttacgca aagtcgtgtt ccggcaagcg
    18181 agctgctggc tcgctttaaa acgcatccgg cagtgcgccc gcttatcaaa aacacggaat
    18241 cactggagta tggtgcgcat ctggtgccag aaggtggcct gcgcagtatg ccggtgcaat
    18301 acgccggtaa cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc aataccggaa
    18361 tttccgtgcg cggtatggat atggcgctaa ctggcgcgca ggcggcggca caaacgctga
    18421 taagcgcctg ccagcaccgc gagccgcaaa atctgtttcc gctttatcat cacaacgtag
    18481 agcgcagcct gctgtgggat gttctacagc gttatcagca tgttccggcg cttttgcaac
    18541 gccctggctg gtatcgggtg tggcctgggt taatgcagga tatttcccgt gatttatggg
    18601 atcaggggaa tacacctgtt ccaccgctgc gccaattgct ctggcgtcat ttacgtcgtc
    18661 acggcttgtg gcatctgacg ggcgatgtta tcaggagtct gcgatgtctg tagcccgtaa
    18721 tctctggcgc gctgccgatg cgccgcacat tgttccggct gactccgttg agcgccagac
    18781 ggcacaacgg ttgattaacg cctgtccagc aggtcttttt tcgctcacac ctgaaggtga
    18841 cttacgcgtt gattatcacg gctgcctgga gtgtggcacc tgccgtttgc tgtgcgacga
    18901 atcaacacta caacagtggc gctatccgcc ttccggattc ggcatcacct accgctttgg
    18961 ataaataata aggataattt atgcccctct tacacctgct ccgccagaat ccggtgattg
    19021 ctgccgttaa agataatgcc agcctgcaac tggcaatcga ctctgaatgc cagtttattt
    19081 ccgtgttgta cggcaatatc tgcaccatta gtaatatcgt taaaaagatt aagaacgccg
    19141 ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa gaagtggtga
    19201 ttcagttttt gaaactggta accgaggcgg acggaattat cagtaccaaa gcctcaatgc
    19261 tgaaagcagc aagagcggaa ggttttttct gtattcatcg actgtttatt gttgattcga
    19321 tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt attgagatcc
    19381 tgccaggctg catgcctaaa gtgctgggct gggtgacgga gaaaatccgc caaccgctga
    19441 ttgctggcgg gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac gcgggtgttg
    19501 tggcgctttc caccacgaat actggggtct ggacgttagc gaaaaaattg ctttgacggg
    19561 ataatcgtaa caagttgaat ttgttgtgat ttgtaggctg cacaccacat ccgacattca
    19621 gcgcctgatt cgacgcttga cgcgtcttat caggcctaca agtccccagc ccagaaccat
    19681 aggcaggatc atccggcagc acgttattgg caatctgcgt gaatgtttgt caccagtgcc
    19741 tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgctgca
    19801 ctggcgtcat gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct
    19861 gctgcgccgg aagcgccaac aacaatacac acctcaggtg ccagcagatg tccggaaata
    19921 ccaatcactt tttcggcatc aacgccaccg ttcatcactc gtgcccgact gtagcccact
    19981 tcggctccca tcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgt
    20041 cccaccacca gcacacgtct ggcctcggtt aatgggtcac gggtaacatt tttcaggttt
    20101 tcgacgctga taagccagtc tggaagcgcg ccggggacaa tgttcagttg ctgcatccca
    20161 gagggcaacg tggcgttttt atctgctccc gcctgacgtg ccagcgaaag gcatagcggg
    20221 cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt
    20281 acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc
    20341 agccgggtcg acagttcatc accaaacgtt cccggcggaa agagaacaac gtccgctggc
    20401 gttcgctgcc actgttccac cagcgcatcc agcacctgtt ccgccactac gggctgaggt
    20461 tcaatctgcc agtgtgccag ggtacaaccg ctgaaatcct gtgccgccag ccagcttgct
    20521 attgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc
    20581 gtaaatagtt ctgccatagc ttttgcgctt tttctgctac cgtttgccca tcaatcagcg
    20641 tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatcgct ggcaattcgg
    20701 cagcaacagt tttacggata atttctgctt tccccgcagc cattcgctgg cgcattcccg
    20761 gtactggcaa ggccacttca ccacactggc gcacggcaat caccgcaggc aagcgaaccc
    20821 ggcaacagcg caacccatgt tcagtacgct gttcgagggt gataaacggc gcgtcgagag
    20881 tgaaacgctc cacctgggtg aaacagggcc agcccagcat ttccgccagc aaaaatggcg
    20941 tttgtccatt ctgcccttcg ctgctttggc agccgataat tatcagatcc agcggattct
    21001 gacgctgcca ttcggcaata tggcgagcga caaattccga cgcaaagcgc agatctgccg
    21061 ccgtctccag cactacagct tcctcaaacc caagagccat gagatagcgc agccagtgca
    21121 acgcccgttc atcccccatg ctcaacgcgg ttaaagacat cgacgtaccg ttttttctct
    21181 gcgccaacag cagcgcggcg gcagcctgtt catcagcacc gagtaaactt cgcagtagcg
    21241 aaacatccgg tccgctatta ccctgagccg ccgcctgcca ctctttttcc gccagcattc
    21301 cggcatccgg ctcggcttta aacgctaaca gaatgttcat tcgcacctcc tacatttccc
    21361 gccgccacca gcgggagtgc tttggtttct ggtgcccata gccaggtcac aacaaaacca
    21421 accaacaaca ctgccgccag gagcaataag gtgacttgca ttccccactg cgccagcacc
    21481 cacggcagca ggccagtact taccgccgcg ccaagtcgac tcatggcggt ggcaaaaccg
    21541 acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta
    21601 ctgactgccg aaatggtggt gctgaagaga acaaaaagca gcagggttaa tgaactgccg
    21661 gaaggcaaac aggccatcac gaccagcgtt gccgccagca gcaaaaaact tcccagcaaa
    21721 aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc cacaattaac
    21781 aacgcattaa gcatcaggct agcggtcaac gcatcttcca gaccaatagt ttgggcgata
    21841 gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa gaagacgctg
    21901 ttaaacgccg tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg tttatgggtc
    21961 gccgttgcca cttcatcgcc cagtaaaaca tgcgggccaa aatagcgatg aacgatagcg
    22021 tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gcgattccgg cgtgccccaa
    22081 cgtagcagcg taatcagcaa ggctggtaac gccgctgatg ccagcagcca gcgccaggcc
    22141 tccgggcttt cggaaataaa gtgatgtccg gcaatacttg ccagcacata gcctacggtc
    22201 cacaccacac tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga aaattcagcc
    22261 agcaaggtgt gaccgactga gtaatcgcct cccagaccaa tgccgatcaa aatgcgcaat
    22321 ccaataagat gctctggcgt ggtggcaaaa aattgcaaga acgaagcgag cgtaatcagc
    22381 ataaaactga aggtgaagat tttttgccgc ccgatatggt cggagatcca ccccaaaacc
    22441 aggctgccaa gaaacaaacc aagtaacgcc gagccgccga tcattcccgc cataaacggc
    22501 gtcagttgca tggcgggcgt aagctgaata atggcgtaac caataacgcc gaggacataa
    22561 ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaattc gacagtgaaa acggttaagc
    22621 ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt gtccggtaat
    22681 aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc ggaacgctgc
    22741 ctgacacatt atttttcaat cggatagata gtacccgtgt tcataatgcc attaggatcg
    22801 aactgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag tttgctccag
    22861 tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg aaccgccgag acgaatggtt
    22921 tcttcgcaga tgattttgtt gagcggattg tggtatttgt cgatttcctc ttccggctta
    22981 cagtcaacga cgttgtagtc gtagacgaag tacatgttgg tgccgttctg atagctatga
    23041 gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcggt acgaatacgg
    23101 ttaataacgc tttcgtagat ttcgtgaatg cagctccagc agccggacac ttcagtggta
    23161 aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagctacttt atccggtccc
    23221 cagttcaggt tgttaaacca ggtttcgatc agcttgctgt ccacgcgctg gcactgcggg
    23281 tagcgcgcca cgatttcagc aatcccttca cccgtcgcct tcgcaatgcg agggttacct
    23341 tcagccataa agatcagcac gcatttgccg tcggcaaaat gggtgaagtg ttgggtgcca
    23401 tcttcagcat catacaaacg agcaatcgac ggacgatacc cttccaccat gatttcacgc
    23461 aggatgttga agccggtttt catgtcttcc agcacatagc catagaagag attgttttcc
    23521 ggcgtaaatt tgaagatttt cactgtgact tcagtgatat agcacagtgc accttcgttg
    23581 ccgatgatga tatgacgaat gtccgggcca gccgcgcgac gtggcacgtt tttaatgcgt
    23641 gtgacggtgc catctgccag caccgcttcc agaccaacga ccatatcctc gattgcgccg
    23701 tagagtgtgg aaaactgccc aatactgcgg gtcgccacca ggccgcccat ctgcgccagc
    23761 ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc gttttccagc
    23821 acctccagcg gaacgccaca ttgcgccgtg gcctgcatat tctcaatatc aatattaatg
    23881 atttgattca tggcggagcc gtcgagcacc accgagtttt ccacaacggt ttccagccca
    23941 ccttcggtgg cggaagcacc ggtacgtggc acaccgttaa ttttgtgcgc attcataaaa
    24001 ttcagcacac gggatacttg ttctgtggaa ccgagtttta cgaccgctgc cggaattggc
    24061 aaagtataaa tgccatgaat atccggaaat ttacgaaaac ggtcaatact gttcttcttt
    24121 aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca
    24181 atcgctacgc gagataaaga catagtaatt ccttcctgat aataaaaatg gaatagttat
    24241 taataagaga aaacagaaac caccgttatg aattagcgca ctaaataacc gccatcaacc
    24301 actaataaat gtccgttgac ataattcgat gccggacttg cgaggaatac ggctgcaccc
    24361 attaaatcct gagtatcacc ccaacggttt gccggaatat gatcaagaac acgctggtta
    24421 gtttctggat tactacgggt cgccagcgta atatcggttg cataataacc aggagcaata
    24481 ccattcacct gaatattata ttggcctaat tcatcgcaat aggctttggt gaatccggca
    24541 agagcatgtt tagtggcaga ataagcaggt gaccattgac cacctaagta agagaacaat
    24601 gaacagatat taatgatttt accgcttttc tgcgggatca taatttttgc cgcttcgtag
    24661 cttaactcga atgcggcggt caggttcacg tcaatcatcg ggtcccagtc ggcacgacca
    24721 aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat atcgacggta
    24781 ccgaagcgct cacagcaggc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc
    24841 acctgcataa aatcaacttc gacaccctgt ttttcaatca tttcctttgt ttcgccgtta
    24901 tctttgacga aactaggaat aaagacattt gcgccagctt tggccaacgc catggcaaat
    24961 gcctggccta aaccgctatt cccgccggta acaattgctg ttttaccttt cagggagaaa
    25021 aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag
    25081 gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct cattatctct
    25141 ggtaactgga gataattttt agcataagga cataactgct aatgtgatcg taatcacagt
    25201 gtgatgttcg tattgtcaaa taatttaaaa aagcagaagc gtgattactc tcacacaaca
    25261 cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa agtaatccac
    25321 ctgaaagggc ggcttacttt tctctttttt tgtctattac aaatgggaat acgatatgca
    25381 acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag
    25441 tttcgacctg gcttatttac gttatattta tcaaattccc atggcaaaat ttatgggatt
    25501 cagcaatacc gagataggtt taataatgag tacctttggt attgcggcca ttattttcta
    25561 tgcccctagc ggcgttattg ccgataaatt ttcacatcgc aaaatgatta cttccgcgat
    25621 gatcattacc ggattactgg gtctgttaat ggcaacatat ccaccgctgt gggtaatgct
    25681 ctgtattcag gtcgcctttg cgataacgac gattttaatg ctgtggtcgg tgtcgattaa
    25741 agccgcatcg ttgctgggcg atcatagcga gcaagggaaa attatgggct ggatggaagg
    25801 gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt
    25861 tgcaccggat gacagcgcca gcctgaaaac ggtcattatt atctatagtg tggtttacat
    25921 tctgctgggc attttgtgtt ggttttttgt tagcgataac aacaatctgc gcagtgccaa
    25981 taacgaagaa aaacagtcat tccagcttag cgacatcctg gccgttttgc gcatcagcac
    26041 cacctggtat tgcagcatgg tgatttttgg tgtcttcacc atctacgcca ttctgagtta
    26101 ctccaccaac tatctgaccg aaatgtacgg catgtcgttg gtggcggcga gctacatggg
    26161 gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac
    26221 ctacagtaaa gtgaaatccc ctacccgcgt ggtccaaatc ctttccatca tcggtttgct
    26281 ggcgttgaca gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg
    26341 cttgatttta ttactgggat tcacctgtta cgcctcacgc gggctgtatt gggcctgccc
    26401 tggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat
    26461 tggattcctg ccggatgtct tcgtttaacc gattatcggc tactggcaag acaccctgcc
    26521 cgctgcagaa gcctaccgca acatgtggct aatgggcatg gcggcgcttg gcatggtgat
    26581 catctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ccgcaatggc
    26641 tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa atacatcata
    26701 gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaatgtg
    26761 gtttgcgaag gcaaaggctt attgcagccg atgcacacgc cagatgccga taccgcagaa
    26821 catcctgacg acgatttatg ggcatcacta tgttttgcca gtcacgattt gatgagccag
    26881 tttgccggaa ataaagaaga tattgtcggc attggcctgg gctcaatccg ttgctgccgt
    26941 gcgttattga aagccgatgg tacgccagcc gcgccgttga ttagctggca ggatgcacgc
    27001 gttacacgcc cttacgaaca cactaatcct gacgtggcct atgtcacctc tttttcgggt
    27061 tatctgacac atcgcttaac cggcgagttt aaagacaata tcgccaacta ttttggtcag
    27121 tggccggtgg attataagag ctgggcatgg agcgaaaatg ccgcggtaat ggaaaagttt
    27181 aatatccccc gccagatgct ttttgacgtg caaatgccag gcactgttct tgggcatatc
    27241 acaccacaag ctgcgctggc gacacatttc ccggctgggc tgccggttgt ttgtaccacc
    27301 agtgataaac cggtagaagc tctgggagcc gggttactgg atgatgaaac ggcggtaatt
    27361 tctcttggca cctatatcgc gttgatgatg aacggcaaag cactgccgaa agatccggtc
    27421 gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg ttacggtatt
    27481 cgcaaaggca tgtggacggt gagctggctg cgcgacatgt taggcgagtc gttaattcag
    27541 gatgccaaag cacaggatct ttcaccggaa gatttgctca acaaaaaagc gtcttgcgtg
    27601 ccacctggtt gtaatgggct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg
    27661 tacaaacgcg gaattatgat cggctttgat tccagtatgg attacgcatg gatatatcgt
    27721 tcgatactgg aaagcgtggc gctgacgctg aagaataatt acgacaatat gtgtcatgaa
    27781 atgaatcact ttgcaaagca tgtgatcatt actggcggcg gttcgaacag cgatctgttt
    27841 atgcagattt ttgccgacgt gttcaacctt ccggcacgcc gaaacgccat taacggttgt
    27901 gcaagtctgg gggctgcgat caatacggcg gtaggtctgg ggctataccc ggattacaca
    27961 acggctgtcg ataaaatggt tcgcgtgaaa gatatcttca taccgattga gagcaatgcc
    28021 aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca tactgatgtg
    28081 atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgcgga ttcgatccag
    28141 agctggtcga atgcgtaagt gagataaaca gtgccgggta gatgattaat cccggcatat
    28201 tcacgtttcc actacaaaat accgggtaac cactgctgaa tattgttctc tcctgagcag
    28261 cgtttatgct ttaattgcta aaccgattta atttgtttag cagttagtat attggagcct
    28321 ttatgagcac aagagtatgg gtactcggtg atgcggttgt tgatttatta cccgaaagcc
    28381 aggggagact actacagtgt cctggtgggg cgcctgctaa tgttgcagtc ggtatcgcaa
    28441 ggctgggggg ggaaagtgcc tttattggca aagttggcga tgatcctttc ggtcgcttta
    28501 tgtatcagac actgagtaaa gaaaatgttg atacacgtta tatgtctctt gatcctcaac
    28561 atcgcacctc aattgtggct gtaggacttg atgagcaagg agaaagaaac tttaccttta
    28621 tggtacgccc aagtgccgat ctttttttac aacctgatga ccttcctaca tttgggccgg
    28681 gtgaatggct ccatctttgt tccattgcgc tcagtgcaga accttcccga agtaccgcat
    28741 ttctggctat ggagaaaata cgtcaggctg gcggaaacat cagttttgat cccaatatcc
    28801 gcagcgatct ctggcagagt gaagcgctat taagaaaata ccttgatcgc gcgctttcgc
    28861 tggcgaatat cgctaaattg tccgaagaag agttgctatt catcagtggc gaaagccagg
    28921 ttcagcaagg cgcatattca ttagtacaac gttattcgtt gactttattg cttatcacac
    28981 aaggaaaaaa tggcgtactt gtgtattttc aggggcagtt tatccactat cccgccaaac
    29041 ctgtttctgt cgtcgatacg accggggcag gagatgcttt tgtcgctgga ttacttgcag
    29101 gtctggctga ttctggaata ccaacaaata ccagacagct tgaacgaatc attgcacaag
    29161 ctcagatttg tggtgctctg gcgaccacgg ctaaaggcgc gatgaccgcc ttaccccgac
    29221 aacacgatct cccttcacaa tagtgatcct gctgttgtat ctgtaacccg atttgtcgtg
    29281 cctgtcacat ttactaaatc ggtttagcga aaataattgc tctggctaaa aagtcagctt
    29341 tattatcaat acctaaaccg gtttagcatt ttagcaattt ttataattct ctgatgggac
    29401 gcgacaaatt atgtataaaa aaacaacttt ggcagtgtta attgctttgc tgaccggtgc
    29461 tacaacggta catgcgcaaa cggatattag cagtatcgaa tctcgactgg cggcgttgga
    29521 acaacgttta aaaaatgcgg aatcccgcgc ccaggcggca gaagcaaggg ccaaaacagc
    29581 tgaattacag gttcagaaac tggctgaaac acaacaacaa aatcagctaa caactcaaga
    29641 agtagcacag agaacagttc agctcgaaca gaaatccgca gaaaacagtg gttttgagtt
    29701 tcatggctat gcccgttccg ggttactgat gaatgatgcc gcttccagta gcaaaagtgg
    29761 gccgtatctg actcccgcag gtgaaactgg tggagctgtt ggccgtctgg gaaatgaagc
    29821 cgatacctat gtcgagttaa atgtagaaca taaacaaaca ctggataacg gtgcgaccac
    29881 acgctttaaa gcaatgttgg ctgacggaca aagagattac aacgactgga ctggcggctc
    29941 cagtaacctg aatatccgac aggcttttgc cgaactgggc gcattaccaa gttttaccgg
    30001 agcattcaaa gacagtactg tctgggctgg taaacgcttt gatcgcgaca attttgatat
    30061 tcactggtta gactccgatg tcgtattttt agcgggaacg ggcggcggta tctatgacgt
    30121 aaaatggaac gatacattcc gcagtaactt ttctctctac ggacgtaatt tcggcgatct
    30181 tgatgatatc gacaataacg ttcagaacta catcctcacc atgaatcatt atgcaggccc
    30241 cttccagttg atggttagcg gattacgggc aaaagataat gatgatcgaa aagatgccaa
    30301 tggtgatctc attcaaactg atgctgcaaa tactggcgta catgcgttag ttggtctgca
    30361 caatgacact ttctatggcc tgcgtgaagg gacggcaaaa acagcactgc tatatggcca
    30421 tggcctgggt gcggaagtca aagggattgg ctccgatggc gctctgctgt ctgaggcgaa
    30481 tacctggcgc ttcgcatctt acggcacaac acctctggga agcggttggt atgttgcgcc
    30541 agcaattctc gcacaaagca gtaaagatcg ttacgtcaaa ggcgatagct acgaatgggt
    30601 gaccttcaat acacgtctga tcaaagaggt aacacagaat tttgctctgg cctttgaggg
    30661 tagctatcaa tatatggatc tgaagccaaa ggggtatcaa aaccacaacg ccgtaaacgg
    30721 cagcttctat aaactcacct ttgctccaac tctaaaagct aacgatatca ataatttctt
    30781 tagccgtccg gagcttcgcc tgtttgccac ctggatggac tggagcagca aacttgatga
    30841 ttttgccagc aatgacgctt tcggcagcag tggtttcaat actggcggag agtggaattt
    30901 tggtgtccaa atggaaacct ggttttaata ccagacagcc tcacggatgt gaggctttta
    30961 ctgaaataac aatgaattag agggttatat ggatttcgat aaaatcgccc aatcgttgct
    31021 tcctttatta ggtggtaagg agaacatcgc cagtgcagca cattgcgcca ctcgcctacg
    31081 gctggtactg gttgacgaca cacttgccga tcaacatgcc attggccaga ttgatggagt
    31141 gaaaggttgt tttcgtaatt cagggcaaat gcaaatcatc tttggcaccg gcgtggttaa
    31201 taaagtctat gctgccttta ttcaggttgc aggtattagc gaatccagta aagcagatac
    31261 cgctcgactc gccgctcaaa aactcaatcc ttttcagcga atagcacggc tactttctaa
    31321 tatttttgtc cccatcattc cagccattgt tgcttctggt ttattaatgg ggctcctggg
    31381 aatggtgaaa acatatggct gggtgaatgc cgataatgcg atttatatcc tgctggatat
    31441 gtgcagttca gccgcattta ttattctccc catccttatt ggctttactg ctgctcgtga
    31501 atttggtgga aatccttatc ttggcgcgac attaggaggg attctgactc acccggcact
    31561 cacaaatgcc tggggcgttg ctgcgggatt tcagacaatg aacttcttcg gttttgaaat
    31621 cgccatgatt ggctatcagg gaacggtttt ccccgtgctt ctggcagtat ggtttatgag
    31681 catagtggaa aaacagttac gcaggtttat ccctgatgct ctggatctca ttctgacgcc
    31741 atttctgact gtcgtcattt ctggctttat cgctcttttg attattggtc cggcaggacg
    31801 agcgttaggt gacggtatct ctttcgttct cagcacgctg attgcacacg caggctggtt
    31861 agcagggttg ttattcggcg ggctatattc agcgattgtt attacgggta ttcatcatag
    31921 cttccacgca attgaagcag gacttttagg taaccctgca atcggtgtta atttcctgct
    31981 gcctatttgg gcaatggcaa acgttgcgca aggcggtgca tgtctggcgg tatggtttaa
    32041 aaccaaagat acaaaaatta aagcaatcac cctaccctcg gccttttccg caatgttagg
    32101 gattactgaa gccgctattt ttggtatcaa ccttcgtttt gttaaacctt ttattgcggc
    32161 cttaattggt ggcgctgccg gcggtgcctg ggttgtctct gttcacgttt atatgactgc
    32221 cgttggtctg accgcaatac ccggcatggc aatcgttcag ccaacatcgt tggttaatta
    32281 tattattgga atggtgattg ctttcgctgt cgcttttagc ctctctttat tactcaaata
    32341 caaaacggac gaggagtaat gtcgatgcct tcacaaatgc ccgcgatttt acaggcagtg
    32401 atgaaaggtc agtccaaagc actggcagat gcccactatc cttgttggca tctcgctcca
    32461 gtcaccggac taatgaacga tccaaatggc ttttgttggt ctggaggacg ctatcatctg
    32521 ttttatcaat ggaatccact ggcttgtgat cataagtaca aatgttgggg gcactggagt
    32581 tcgacggatt tattgcactg gcagcatgaa ccattagcgc taatgcccga taaagagtac
    32641 gaccgtaatg gttgttactc aggaagcgcg gttaataatc agggcgttct cacactttgc
    32701 tataccggta atgttaagtt tgatgatggc agcagaacgg catggcaatg cctggccacc
    32761 gaaaataacc agggcgggtt tgataaacta ggccctgtca taccgttacc tgatggttac
    32821 accggacatg ttcgtgatcc aaaagtctgg aagcacaaca gccaatggta catggtgctt
    32881 ggcgcgcaag ataaagagaa acgggggaaa gtgctgctgt attcctctgt cgatctctat
    32941 acctggtctt ttcatggaga aattgctggt aacggtctga atgaaataga taacgcagga
    33001 tacatgtggg aatgtcctga ttttttcgcc cttgatggtg aatacattct cctctgttgc
    33061 ccccagggaa tggtgcgcga acatgagcgc tatctgaata cttatccctg cgcctggcta
    33121 catggacagt ttgattacga gacaggcaaa tttacgcatg gcgctttctc agagctggat
    33181 gcgggatttg aattctatgc accacagacg atggaagccc cggacgggcg acgattgctg
    33241 gtcggctgga tgggggttcc tgatggcgaa gagatgttgc agccaaccag aaagcatcat
    33301 tggcagcatc aaatgacctg ttttcgcgaa ctctcattcc agcagggaaa attgtttcag
    33361 atgccaatca gggaactcgc gcaactgcgt gaagctgaac acttctggca aggaaaagcg
    33421 gatcatgcgc cgcatgtcga aattgaacgc ctggaaatgg acatcattcc gtcgggtgag
    33481 ctgtatctga attttggcaa tgcactggca ctgcatctta atgacgatgg tattcaacta
    33541 cagaggagaa gccttgcagg gcaagagaaa ttaactcgtt actggcgagg aagcgtaacg
    33601 tcactgaaga ttctctgtga cagttccagc gtagagatat tcatcaataa tggtgaaggt
    33661 gtaatgagta atcgttattt tccccatcat ccagcttcgc tgatattaca gggtgagtct
    33721 gacgtcacgt tacgctactg gtcgctacgc gcctgcatgg tagaatgagg gttttgaatt
    33781 acggatcctc gtcgtgagaa aaaccaaacg cgttactatt aaagacattg ctgaactggc
    33841 gggagtgtcg aaagcaaccg ccagtctggt tctcaatggt cgtggcaaag agttacgtgt
    33901 tgcgcaggaa acgcgtgagc gtgtgctgag tattgcccaa cagcagcatt atcagcccag
    33961 cattcatgcc cgctcattac gggacaaccg cagtcatacc attggtctcg tcgttcctga
    34021 aatcactaac tacggatttg cagtattttc ccatgaactg gaaacattgt gtcgggaagc
    34081 gggcgtccag ctcctcatat cctgtactga tgaaaatcct tcacaggaaa caatggtggt
    34141 gaacaatatg attgcccgcc aagttgatgg gttaatcgtc gcatccagta tgctgcatga
    34201 taatgactat caaaaactta gcgagcaatt acctgttgtg ttgttcgatc gccacatgaa
    34261 cggcagtaca ttgccattgg tcattactga ctctgtttct cccaccgccg ctctggtggc
    34321 agatattgca cggagtcacc cggatgaatt ctattttctg ggcggacaac cccgtttgtc
    34381 accgactcgc gatcgactgg aaggatttac acaaggcttg cagcaagccg gagtaacgct
    34441 acaacctgaa tggattattc acggtaatta tcatccgagc tcaggctatg aaatgtttgc
    34501 cgcgctatgc gctcgtttag gacgtccccc taaagcctta ttcactgccg cttgtggatt
    34561 actggaaggc gttctgcgct atatgagcca atataattta ctcgacagta aaatacactt
    34621 ggcgagtttt gatgatcatt acttgtatga ttcactttcg gtaagaatcg acacaataca
    34681 acaggataat cgccaacttg cttttcactg ctttgaactt atttcgcaac tgattgaagg
    34741 tgaaacgcca tccccattac agcgttatct tcctgccagt ctacaaaaac gctaccgata
    34801 atcccgtcac tatcttgcat cgcccctgta aaacggggcg attattttca tcctgaacgt
    34861 taaatattaa tgatgaataa tcaaatcagg caatattcaa atatttatgt gtctgcatcg
    34921 acaaacgcca gttgcgcgca atgcaggtat cgatacataa acgcgtagca tcttccttct
    34981 ggctaattgg ctgaagtgca atgactcgcg gtttatcatc ggtcagcgtc gccagcaatt
    35041 cgtccagtgc ttcgatatca cgtacgcgtc caactgggtg tttgatttcg ttagctcgct
    35101 ccagggcttg tgacaacact tcatagccgc cgcgcatgtt cagctttggc gatacggtaa
    35161 cccaggtatt cggtgtgcag cgtacctcat gagtaccact ggtttcgatc tggcagctaa
    35221 aaccgttctt ttcgagcagg tcagtcagtg gcagcaaatc atgaatgcaa ggctcaccac
    35281 ccgtaatcac cacatgacgc gcggtgtatc cctgacggcc aatgacagcc agcaaatctt
    35341 cactgctcgc cgccccccac ttatcactct ctttggtctt cgccagaatg ctgaaaaggg
    35401 agacttcccg atcctcaagc ttttcccagg tgtgtttggt gtcgcaccag gcacagccaa
    35461 ccggacatcc ctgtaaacga ataaaaatgg cggggacgcc ggtaaagtaa ccctcacctt
    35521 gcagggtctg gaacatctcg ttaatcgggt actgcatagc attctctgtg aagtggataa
    35581 ttgttaatta ttgcagatcc tgccacaaca atcatgtctt attaacattc tgttacaggc
    35641 aggtttgatg caaaaattgg ctcagatcat taagaataaa atgaagcggc gtattatgtt
    35701 ttaaggaatt aattaaaagg aatttaacca tgctcagaat attcatcgca attatattta
    35761 ttttcaattt atcaggctgt ggatataacg atattcaaac ctatgatgag caggttaacg
    35821 cctcctggtc tgaggtattg aatcaatatc aacgtcgcgc cgatcttatt cctaatcttg
    35881 tcgccagcat taaaggttat tccagccatg aacgagatgt actggaagcc gtaacattgg
    35941 ctcgtagcca ggctaatcgc gccagtagcg atcttcagca aactccggga gatgaacaaa
    36001 aattacaaac ctggcaacaa gctcaggcgc aggtgtctcg cactctcggt cagttaacga
    36061 taattagtga acgttaccct gagctaaagg cccaggaact ctatcaaaat ttgatggtac
    36121 agctcgaagg aagtgaaaat cgaattgccg ttgcgcgagg aagatatatt aaagctattg
    36181 aacaatataa tgtcactatc cgcaaattcc ccgcagtgtt aaccgcaaag gtcatggatt
    36241 acacaccaaa gaaaaattat ttgccggatg atgtcgccgc tgtgagtaaa gccccgacaa
    36301 tagattttag ccagaatact aatgctcatt aagcggggaa gtcctgtgcg aatttttatc
    36361 ttccttatgg ctttcttctg ttcatctgtg tttgccacac aaattccagt gcctgaatca
    36421 cccaggtatg tgaatgactt aaccggtaaa ttgacgaacg atgaagtaaa tactttgacc
    36481 aatcagatta aagagctgac gcaaaaaagc cacgcacaac tggtcgtgtt agtggttgaa
    36541 acaacgggcg atgaaaccat cgagcagtac gctacgcggg tattcgatag ctggaaacca
    36601 ggtgataaag accgcgatga tggcgttcta ctgttagtcg cctggcagga tcataccgtg
    36661 cgtattgaaa tcggttacgg actggaaggc gttattacgg atgcgcagtc cgggaaaatt
    36721 attcgcaaca gtattattcc ggcatttaaa aatggcgacc tggctggcgg cttgcaaaag
    36781 ggaataagcg atattgataa tcgtctgacg gggaataatt tagcaacgat aacaccgccc
    36841 gatcaccctt tgccctttaa cggttggtgg gctttgttag tctgggcaat agtactgacc
    36901 tttatttccg ccagaggata tttcaaaacg ctgggactaa tttgcttcac agctattgta
    36961 ctggcctttg ttttacctat agcaggtttc agcggaagct ggggagtact cgcaacatta
    37021 ctctgcttcg ctacaccgtt cctggctgtc gctatcgctt ttaccccctt cggcaaaaag
    37081 gttcgggatt cgatgcgtag tgccaaccga ccgtcacgtt acgaaagaag taactcggcc
    37141 tcctggcgtt cttcatcatc gtcgtcatct tcctcttcat cctcttttga caatgataat
    37201 ttcagcggcg gcggcggttc atctggcggc ggtggcgcct ccgggcgctg gtaacaaaag
    37261 gaattgaaat gcgtattttt ctcttacttc tgatgctata tggtttccag gcgttagctg
    37321 aacaattgcc ttcagtaact gacttaaccg ggacattaac gacagaagaa caaactgctt
    37381 taaatcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc gtgttggtta
    37441 cgccgaccac agggggcaaa aacattaaac ttgcagcctc tcagagctac gggttatatc
    37501 actggaaacc cgttggagag aagcggtacg gcgaaggtat cttaattctc gtcgtttggc
    37561 cggaagggct ggcgtcaatg aagattgggc atggactgaa agagatgctg ccaccggaac
    37621 aagctgcgca aattgttcat taccatatgc aaccggaatt caaaaaaaat aatctcttct
    37681 ccgggttaac gcagggaatt gaaggcatcg cacagtttac ctatataaaa gcgaatctta
    37741 gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaacgctcg ctgccatgtc
    37801 ttgcctggac attactgatg atcgttgcca tcgttgtcct ctggcgattt accagccgtc
    37861 ccgggcctgg catatggatg atatcgatga ttactcctat ggtctggttt ttcttctttc
    37921 aggatgatgt catcattcgg cgggtatcgc tcgtttgttt tagtttattt ttcgcagcaa
    37981 cctgctggca gcgtatcgcg gtaatttttc atatgtatcg cgtatttcct atgatgctgg
    38041 ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagac gactcagcag gtttctcaat
    38101 ccgaacgaaa ctcattgttt gtcgtgctgc tgataattgt tgtgggatgg tgcctggtat
    38161 tcgccagtaa taaagatata gcattcatct caacaattat tgggctaatt gatcatttta
    38221 tcggcccaat aaccattgct ggcatcgttc tgctgatcat tgtggccaaa tttactggca
    38281 atcttaaact gggatcagga cataaaagca acaggaaaac ggggaatcgt aatgctcatt
    38341 ctcgctcttc gtcgaaaaat tctttccgcg gtggcggcgg ttcttcagga ggcggcggtt
    38401 catccgaccg ttggtgatcg ttatgcgtat aatccttttt ctactcactt tatggtgcct
    38461 tcctgtgctt gcccagcaaa ttgctgtgcc ggagctgcgc caacaggtga ccgatattac
    38521 tggtacctta agcacgtctg aacaacaatc actaacacaa cagctgcagg atattacaca
    38581 aaaaacacgg gcacaggtcg ctgtattaat agtgccatcc accggagacg attctattga
    38641 acaatatgct acaagagttt ttgacaattg gcgtttagga gatgccaaac gcaatgatgg
    38701 gatactgatc attgttgcct ggtcggatcg cactgtccgc atccaggtag gttatgggct
    38761 ggaagaaaag gtaaccgacg ctctggctgg agatatcatc cgtagcaaca tgatacccgc
    38821 atttaaacaa caaaaattag ctaagggatt agagttagct ataatcgctt tgaataaccg
    38881 actcacttca caacaccaat attcggttaa tccttcagaa agtgaatcag catcttccag
    38941 cgatcattac tactttgcta ttttttgggt atttgcagtg atgttcttcc ctttctggtt
    39001 ttttcatcaa ggcagtaatt tttgtcgcgc atgtaaaagc agtgtctgta tttcagcaat
    39061 ctatctttta gatttattcc tgttctccga taaaactttt tccattgctg tatttttctt
    39121 cttttttact ttcaccacca ttatggtctt tacctgttta tgtgtacttc agaaaaaagc
    39181 atctggtaga agttatcatt cagacaacag cggttccggt ggtggctcaa gtggcggcgg
    39241 atttagcggc gggggcggtt cttctggcgg tggcggcgcg tccggtcgct ggtaactcaa
    39301 ccataaaaaa tgccagcccg aaggctggca tttttaaatc agataaagtc agacttatgc
    39361 ctggcctttg atctctttac gaccgttgta cggtgctttt tcgcccagag cttcttcgat
    39421 acgaatcagc tggttgtatt tagcaacacg gtcagaacgg ctcatagaac cagttttgat
    39481 ctggcctgca gcagtaccaa cagccaggtc agcgatggta gcgtcttcag tttcgccaga
    39541 acggtgagag ataactgcag tgtagccagc gtctttcgcc atcttgattg cagccagagt
    39601 ttcagtcaga gaaccgatct ggttgaattt gatcaggatg gagttagcga tacctttttc
    39661 gataccttct ttcaggatct tggtgttggt tacgaacagg tcgtcaccaa ccagctggat
    39721 tttgtcgccc agaactttgg tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc
    39781 gtcttcgatg gaaacgatcg gatactgttt ggtcagttct tccaggaagt gagtgaattc
    39841 ttcagaggtg aacgctttgt tgccttcgcc agccagaacg tatttaccgt ctttgtagaa
    39901 ttcagaagct gcgcagtcca tcgccagagt gatgtctttg cccagttcat aaccagcagc
    39961 tttaacagct tcagcgataa cagccagagc ttcagcgttg gaacccaggt tcggcgcata
    40021 gccaccttcg tcaccaacag cagtgttcat gcctttcgct ttcagaactt ttgccaggtg
    40081 atggaaaact tcagaaccca tgcggatggc ttctttcaca gttttcgcgc caaccggctg
    40141 aatcatgaat tcctggatat caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt
    40201 catcatcgga accggcatag agtatttgcc cggagtgccg ttcagttcag cgatgtgctc
    40261 gtacagcggc atacctttag cagctgcagc agctttggcg ttagccagag atacagccag
    40321 gattgcgttc gcgccgaatt tggatttgtt ttcggtgccg tccaggtcga tcatgatctt
    40381 gtcaatgcca gcctgatctt tagcatcttt gccaatcagc gcctgagcga tcgggccgtt
    40441 taccgcagca acagctttgg ttacgccttt acccaggaaa cgggatttgt cgccatcgcg
    40501 cagttccaga gcttcacggg aaccagtaga agcacctgac ggagcagctg ccataccgac
    40561 gaaaccaccc tccagatgta cttcggcttc aacagtcggg ttaccacggg agtcgatgat
    40621 ttcacgaccg atgattttta cgattttgga cattaggttt tcctcaagtc actagttaaa
    40681 ctgaaactcc agacaaacaa cgcgtaccca gggtacgcgt tgccgttcta acttttttac
    40741 ttacttcgcc tgacgtttct ggaactcgct ggcggctttc acaaagcctg caaacagcgg
    40801 gtgaccatca cgtggagtag aagtaaactc cggatggaac tggcaagcca cgaaccacgg
    40861 atgattcgga acttcgatga tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg
    40921 cagacctgca tcttcaatct gtttcaacag catgttgttg acttcgtaac ggtgacgatg
    40981 acgctcaaca attgtcggcg cattgtacag ctggcgaacc aggctatcgt caaccaactg
    41041 gcactgctgt gcgccgagac gcatggtacc gccgagatcg ctcttctcgc tacgaacttc
    41101 aacgttgccg ttttcatcgc gccactcggt aatcagcgcc acaaccgggt acttacagtc
    41161 tggcacaaat tccgtagagt tggcgttctc catgttggca acatggcgag cgtaatcaat
    41221 taacgccacc tgcataccca ggcaaatgcc cagataagga atattgttct cacgcgcaaa
    41281 acgcgcggtc gtaatcatgc cttctacgcc acgatagccg aaaccgccag gtacgaggat
    41341 tgcgtccaga cctttgagga tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag
    41401 tttgatgttg acgctgacac gattcttcag cccaccgtgt ttcagtgctt cgatcacgga
    41461 tttgtatgca tccggcagct caatgtattt gccgaccata ccaatggtga cttcacttac
    41521 cggattcgct tcttcgaaga taacctgttc ccattcggac agattcgctt ccgggcagtt
    41581 taagctgaat cgtttacaaa tataatcgtc cagcccctga gatttcaaca ggcccggaat
    41641 tttatagatg gaatcgacgt ctttcagaga aataaccgct ttttccggaa cattacagaa
    41701 caatgcaatc ttcgcacgtt cgttcgccgg aacggcgcga tctgaacgac aaatcaggat
    41761 atcaggctgg ataccgattg aaagcagctc ttttacagag tgctgagtcg gtttggtttt
    41821 gacttcacca gacgctgcca tgtacggcac cagcgtcagg tgcataaaca gggtgtgctc
    41881 acggccaatt tcaacagcca tctggcgaat cgcttcgagg aacggcaggg attcgatatc
    41941 acctacagta ccgccgattt cgaccagtac tacgtcatga ccttcgccgc cttccagcac
    42001 gcgctctttg atagcgttag tgatgtgcgg aataacctgt acggttgcgc cgagatagtc
    42061 accgcggcgt tctttacgca gaacgtcaga gtagatacgc cccgtggtga agttgttgcg
    42121 gcggctcatt ttggtacgaa tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc
    42181 gccgtcttca gtaacgaaca cttccccgtg ttggattggg ctcatagtac cgggatcgac
    42241 gttgatgtac ggatccagtt tcatgatggt cacattgagg ccacgggctt caagaatggc
    42301 tgcgagggag gctgcggcaa tgcctttacc cagagaggat acgaccccgc cggtcacaaa
    42361 aatatagttc gttgtcatgc tgaacctgag aagttaggtt gaaaagacga tggaataacc
    42421 aggacgggaa agcagtatac ccgaacatga cctgtgccac aaactttcat tatccctcct
    42481 cttcgccagc gcactattga aatcaggagt gagaaaatag cccctttggg gtaaatgttt
    42541 ttgacgcaaa tcaagcgctt gtcatttaaa aaatgacaca aatcgcgctt gaccgcgtaa
    42601 ttcccttaga gatcaatttc ctgccgtttt acctgttgcc agacttcttc cattgtttcg
    42661 aggtcaacac ctgtcatttc cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa
    42721 cgacgctcga atttttcgtt cgctttttgc aatgcgattt ccgcttttgt ccctaaatga
    42781 cgagccagat tgaccgtggc aaacagcagg tcacccattt cctcctccag tttagcctgg
    42841 tcgacaacag cctgccgcgc ctcgtacatc acctcgtcga tctcttcgta gactttatcg
    42901 actaccggac caagcgtcgt ccagtcgaaa ccaacgttgg cgcaacgttt ctgaattttt
    42961 tgcgcacgca ttaaagccgg taaactacga ggaatatcgt ccagcgccga atgctgcgct
    43021 ttctgcgcgc gctcttcggt tttgatttgc tcccaacggg caagcacttc actactattt
    43081 tcggcagaac tgtcagcaaa aacatgcgga tggcgacgct ctaatttatc gctaatagca
    43141 gcgcaaatat cattaaagtc aaagcgccct tcttcctgag ccatttgtgc gtaaaacacc
    43201 acctggaaca gcagatcgcc cagttcaccg cgcagatcgt caaaatcttc acgggcgatg
    43261 gcgtccagca cctcgtaggt ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc
    43321 tctttatccc acgggcagcc gttttccggg tcgcgcaggc gctgcatgat ggtgagcaaa
    43381 cggtcgattt gattcattga attatcctga aaattgcggg tccgtcaggt ggaaacctgt
    43441 gaccagaata gaagtgagtt agtaacacct acccaatcag cacgttaatt ttggctttaa
    43501 tgagttgtaa ttcctctggg gcaactgttc ctttcttcgt tgctcctctt gcccgccagg
    43561 cgattctttt tacctgatca gctaacgcta cgccatcacg ttcctgaccg gatagaacaa
    43621 cttcgaacgg atatcctttt gattgcgttg tacaaggaac acacagacac atacctgttt
    43681 tgttgttgta catgaacgga ctcaggacaa cagccggacg atgtccggct tgctcgctac
    43741 cttttgtcgg gtcaaaatca acccaaatca gatcgcccgt atcgggtacg tatcggctta
    43801 ccattaccag acttccttat ctttcggctc cccccagtcg atattctcgt ggaggttttc
    43861 cggcgtgatg tcgttgacca gttcagcaag cgtaaatacg ggctctttac gcactggctc
    43921 aataattaat ttgccatcca ccaggtcaat cttcacttca tcatcaatat tcagattgag
    43981 cgcctgcatt aacgtagccg ggatccgcac cgccggtgaa tttccccaac gctttacgct
    44041 actgtggatc ataacccttt cctcaaaccg cgatcatatg tagatacagt atatatcaat
    44101 ctacattgta gatacgaaca agtttcggcc taactcccgt gcaaccgacg cgcgtcgata
    44161 acatccggta cctggttgag tttacccagc acgcgaccca gcacttgcag gttgtaaatc
    44221 tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca
    44281 agtacgttca ccttctcgtt ggcgagaatg gtcgtgatat cacgtaacaa cccactacga
    44341 tcattagcca ccacgcggac caccagcgaa tatccggcgg agtagctctc gccccatacc
    44401 gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg ccaattgttc gcaatcggcg
    44461 cggtgtactg aaataccgcg cccttgggta atgaagccga caatctcatc tccaggaatc
    44521 ggctggcagc agcgcgcgat gtggtgcatc aggttaccga ccccttctac caccacacga
    44581 ccgttatctt tactgcggtt ttgcggcgtg tagctttttt gctgaagctg tttcagcgcg
    44641 gcggcgtcct gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc
    44701 tgattgagac ggatatcccc gccaccaatc gccgccagca actcgtcgac atcattgaag
    44761 ttgtaacgcg gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc
    44821 tcgtcgtcaa ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac
    44881 caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac
    44941 cagtcacggc tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc
    45001 agctggtagg tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg
    45061 acatcactgt ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg
    45121 acatcacctt tcggcgtaaa gacgtacact cggtcgtcaa agacctgact acgtacttcg
    45181 tcgagcattt cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag
    45241 gcaatccggt cttcatgtcc cgaacgtgcg ccgccagcag ccgcgccctc tttatatttc
    45301 cagtgcgcag caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc
    45361 tcaacggttt tgccacccgg ccccagaacc acggtatgaa tggactgata accgtttggt
    45421 ttcgggttag cgacgtaatc gtcaaactca tccggcaggt ggcgatagtg agtgtgcact
    45481 atccccagtg cggcataaca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca
    45541 tcaaacagct catcgaaggc gaggttcttt ttctgcattt tgcgccagat actgtagatg
    45601 tgtttcggac ggccatacac ttcagcttta acgccttcag ctttcatctc agcgcgcaga
    45661 tggccgacaa actcttcgat atagtgttcg cggtcgagac ggcgttcatg cagcagtttt
    45721 gcgatgcgtt tgtattcggt cgggtggaga taacggaagc agtaatcttc cagctcccat
    45781 ttcagttgcc caatcccaag acggttcgcc aacggcgcgt agatattggt gcactctttt
    45841 gccgccagta cacgttcatc ttccggcgca tcttttactt cgcgcagatg agcaatacgc
    45901 tccgccagtt tgatgactac gcagcgaaaa tcatcgacca tcgccaataa catccggcga
    45961 acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc
    46021 gccgccatat cacgcacgcc gtgaataagg ttaacgaccg acttaccgac gctatcacgc
    46081 agcacatctt cgctgactac gttggcatcg gccagaggga acagcagcgc cgcccgcagc
    46141 gtgtcaatgt ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat
    46201 aacagactgg catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct
    46261 aagcactcac acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca
    46321 aattcaccag ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg
    46381 gaccagacct gccgaaatcg gcaaatcgta actatttaac gcgcgagaaa agtaccatcg
    46441 attccagatg tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc
    46501 ctgcttttaa taacgcttcg ctatcccgag ccagcgttgc agggttacag gatacataaa
    46561 ctatacgaat aggttccagt tttataattt gctgcataac acctgcggca cctgctcgcg
    46621 ccgggtccaa caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt
    46681 cttcaagatt ttcgtgataa aacgtcacat tctgtaagcc attaagacgc gcattctgct
    46741 ggcctttttc caccagcgcc ggaacacctt ctacacccac cacactggca gcttgtgtcg
    46801 ccaatggcag tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt
    46861 ccacatccag ccattccaac gcacgcgcta ccattttctg gttcacaccc gcattgacct
    46921 gaataaaatc gcgcgggcta aaagttaagc gcaacccatt tgagtcatac cagggcatct
    46981 caccagagac ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag
    47041 aatgcgaaaa gcattccagt ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca
    47101 aaatcattag cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgcatag
    47161 cttgtaagct gcccaggcat gccctgactt tgggcagcaa tgcttcaagt tggggcacta
    47221 aaatggggca ttgtttcacg tcgacaatgt cactggagcc cgctttgcga aaccccatct
    47281 gaagttgctg tgttttcggt aagtagttca gacttaaacg cgcgcgacgg cgatagcccc
    47341 agggaacatc ggcgatcact tcagagactt cgtgtttcat taatcgggcg agtgccgcac
    47401 ttttgcttcg ctgctgtaaa tccacactgg cgtgttgttg ctgacagccg ccgcatacgc
    47461 caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc gcttaaccga cgtacgactt
    47521 tagcgcgggc atactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgcggca
    47581 ataatccggg gataaataat gttttgccgt tatgtcgcgc cacaccctga ccaaaagagt
    47641 cgaggtcgtt gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt
    47701 agaattgcgc cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa
    47761 cggaactcca tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg
    47821 accgtcctta ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg
    47881 cagcgtcaac tggaagatgc cggtgccagt attattgagc cgcttgcagt ttctactgaa
    47941 tatggcatga gcctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt
    48001 cgccattccg atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc
    48061 acctccaatt ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct
    48121 cgccagctca ctgtcactcg tgacggcgat attatgatcc tccgcacgcc gattatttct
    48181 gaaagttact cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgctg
    48241 ggatatattg cgctggagct ggatcttaaa tcggttcgct tgcagcaata taaagagatc
    48301 tttatttcca gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc
    48361 ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt
    48421 cgcgggcaac tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa
    48481 aacggtatca actcgatggc aatgtcgctg gcggcttatc acgaagagat gcaacacaat
    48541 atcgaccagg cgacgtccga tctgcgtgag acgctggagc aaatggagat tcagaacgtt
    48601 gagttggatc tggcgaaaaa gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg
    48661 gcaaatatgt cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg
    48721 acgctgaaaa cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg
    48781 gcaaataatt tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagccggt
    48841 aagctgattc tggaaagtat tccattccca ctacgcagca cgctggatga agtcgttacc
    48901 ctgctggcac attcttctca cgataaaggg ctggaactga cgctcaatat taaaagcgac
    48961 gtgcctgata acgtgatcgg cgacccacta cgattacagc aaatcatcac taacctggta
    49021 gggaatgcaa ttaaattcac cgagaatggc aacattgata ttctggtaga aaaacgtgcg
    49081 ctgagtaata ccaaagtgca aattgaagtg cagattcggg ataccggcat tggtattcct
    49141 gaacgcgatc aatcgcgctt attccaggcc ttcagacagg ctgatgccag tatttcccgc
    49201 cgtcatggtg gcaccgggct ggggctggtg attacacaaa aactggttaa tgaaatgggc
    49261 ggcgatattt cgttccatag ccagccgaat cgcggttcaa ctttctggtt ccacattaat
    49321 ctcgatctga acccgaacat tattatcgaa gggccatcca cccagtgcct cgctggtaag
    49381 cgcctggcct atgtcgaacc aaactccgca gcagcacaat gcacgctgga tattttaagt
    49441 gaaacgccgc tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac
    49501 gacatgatgt tattaggcat agcggtgacc ttccgcgagc cgctaacaat gcaacatgag
    49561 cgattagcga aagcagtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca
    49621 caagtcaatg ctgaaaaact taagcaagat ggtatcggcg cgtgtctgct gaaaccatta
    49681 acacctacgc gcctgttgcc tgccctgacg gaattttgtc atcacaaaca aaacacgctt
    49741 ttgcctgtaa ccgatgaaag taagctggca atgacagtca tggcggttga tgacaacccc
    49801 gctaacctga aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc
    49861 gatagcgggc atcaggcggt tgaacgggcg aaacagatgc cgttcgattt aatcttaatg
    49921 gatattcaaa tgccggacat ggacggcatt cgggcatgtg agctcatcca ccagctcccg
    49981 catcagcagc aaacgccggt tatcgcggta acggcgcatg cgatggccgg gcaaaaagag
    50041 aaactgcttg gcgcggggat gagtgattat ctggccaaac cgatcgaaga agagcgattg
    50101 cataatttgc tgttgcgcta caagcctggc agcggtattt cctctcgcgt cgtgacgccc
    50161 gaagtcaacg aaattgtggt caacccgaat gcgaccctcg actggcaact ggcactacgc
    50221 caggcagcag gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg
    50281 cctgaagttc gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt
    50341 gatttgattc ataaactgca tggcagttgc ggctatagcg gtgtaccgcg tatgaagaat
    50401 ctctgccaac tgatcgaaca acagctacgt aacggtacta aagaagaaga tttagagccg
    50461 gagctgctgg aactgttgga cgagatggat aatgtagcgc gcgaagccag caaaattctc
    50521 gggtaatgga cgtcttgtcg agtagcaacc ggatttttat gacgctggtt tcagattggt
    50581 gacaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc ttatccggcc tgcaaaacca
    50641 attaaattca atgaattaaa aaatgatgta gccctgatac gtagcgcaac aggcaatttt
    50701 gcgtttggca tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa
    50761 cgcaccatgc acggacgctt gttatcgaac gtccagccgg ggatcaggta ctgcattccc
    50821 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcgtgcgc tttcatcact
    50881 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgaac cagaccgcct
    50941 ttgatctcta acggttcttt ggtcaggcgc tgattacctt cctgccagat ccagtgcgta
    51001 tcaatcgcgg tgattttacc cggtgcggca gcggcaacat gggtaaacat cgccagggaa
    51061 atatcgaagt ggttgttgga gtgtgaaccc caggtcaggc caaattcatg acacatttgc
    51121 gccacacgta ccgaaccttg catcgtccag aaatgcggat ccgcaagcgg gatatcaacg
    51181 gattgcaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgcg
    51241 gtcggcaggc cagttgcgcg acggaactct gccatcactt cacgcccgga gaaaccttgc
    51301 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtatgt accgatttta
    51361 atcgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaaa
    51421 cgttgcgcca gtgccacaat agattcggct tcttcttccc cggccagtac accgcctttc
    51481 agtttgaaat cgttgaagcc atatttttca tacgccgctt ccgccaggcg caccaccgca
    51541 tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg
    51601 ctttgatacg gcagcggtgt agctttgcga ttaccgacga agaacagata accgagcatt
    51661 tcgacttcgc tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc
    51721 tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    51781 aaatcaaatg tctgcaaacc gcgcccaccg gcatcacgat cggcaaaagt attacgcacc
    51841 agcgtcagaa cgtttttgta ttcacccagc gttttaccca ccaccagcgg aatcgcatct
    51901 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa
    51961 ttatctttga taatcacaat attacgcgtg aagaacggtg catgtgcacc actcagattc
    52021 atcagcatac tgtcatgacc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    52081 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac
    52141 gtttacggtc aaaggtccag ccggggatca ggtactgcat cgggcctgcg tcgttacgcg
    52201 caccgccagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    52261 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    52321 ttttggtcag acgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    52381 tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatca aagtggttat
    52441 tagaatggca gccccaggtc agcccccagt cgtcgcaaag ttgcgccacg cggactgcac
    52501 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    52561 catgacccat ttcgcgccag ttggtggcga tcatgttggt cgcgacgggc aagccggtcg
    52621 cccgtcgaaa ctccgccatc acttcacgac cggagaaacc ctgctcagcg ccgcacgggt
    52681 cttccgcata ggtaaggaca tcattcagcc ctttgcacag agaaatggct tcatcaagca
    52741 gccaggcacc attgggatca acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac
    52801 gaacagtgtc gatttcttgc tcgccaggta acacgccacc cttaagtttg aaatctttaa
    52861 agccatagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    52921 tctgatgccg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    52981 cggttttggt ccgatcaccg atataaaaca gataaccgag aacggtaata gcatcacgtt
    53041 gcttgcctgg ccctaacagt tcgcagaccg gaacattcag cgcctgacct aacaggtcaa
    53101 gcaaggcggc ttccagcgcc gccacggcat taacgcgcaa ttcaaaagtc caggcacctt
    53161 tgccgaaggt atcaaaatcg gctgcctgat tacctttatg cgcctgctga accactttat
    53221 tcaggcgcgc aacttcctgg cccagcacca tcggaatagc cttgaccagt gtttgataaa
    53281 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcattatcg gtgagtacca
    53341 caatattgcg ggtgaaatat gcgttatgtg caccaccaat attaagcaac atgctgtcat
    53401 gtccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgtcatct
    53461 tattgccccg cgacaggttt cagctcaata cgcttgatat cgcccaccag caccaggtag
    53521 ctgagtaccg cgattaaggc atgaacacca acataaatca gcgccccatt aaaggagcca
    53581 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccaaac
    53641 atgttaaaca ggccaccgga aagaccgctg atctcttttg gcgcagtatc agccattaca
    53701 gcccaaccca gcgcaccgat ccccttaccg aagaaggcca gcgccataaa gccgatgatc
    53761 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatc
    53821 acgatcggtg ttttacgcgc aatgttcagt gaacccgtgc gacgcatcag ccagtcggaa
    53881 ataatcccac ccagcacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    53941 cccgctttca gaatcgacat cccgcgcgcc tgcaccagat aaaccgggaa ccaggtaata
    54001 aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    54061 gacccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    54121 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tgtactccag ctctttctga
    54181 tttacgcccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    54241 atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg agtcaaccag
    54301 cccataatcg gcgcgaagat aaccgttgcg aagtattggg cggagttaaa aatcgacacc
    54361 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcggctgtt gccagggaaa
    54421 gagggcgctt cagcaagccc gaccaggaag cgcagtgtaa acagggcaac gataatgccg
    54481 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    54541 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accagccacc agggatctgc
    54601 ccgataacat acgcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    54661 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagcgcggtc gccgtagttg
    54721 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    54781 acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    54841 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgtttttgc
    54901 cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat agcagtataa
    54961 aaagcgcgcc atagcggctc accgtgcaac aacacaacat taatgcgttc aatgaggccc
    55021 gattttggca ttagccctgg acggtggaat ccacttcacg gaaatgaaaa caagaacaag
    55081 aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa atggatcgct tgactccagg
    55141 caaacgccag taaaaatccg cgctatgaag cagtttttac tggcatttgc ctgaaaagat
    55201 tcgattcagc accgctaaaa cggcatttac cgctcgctga acatatcagg acaacatcgt
    55261 gccccaatgt tcaacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    55321 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    55381 tttgccgcca ttgcagaaat tcacataact actgtcgccg agcgcaatca cgccataacg
    55441 cagattcggc tggaagccca gactatcttt gattccctga aagagtggca caatactatc
    55501 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    55561 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gcgcggtcag
    55621 aatcgcttcc gcttcttcgg ccactaacag tgaattcccg tacatggtgc cgacaaaaat
    55681 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    55741 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcacca gccaaattgt
    55801 gataatgcct gcatccaggt gtcgtccagg cccgcgtgaa tagccagcgg ctcgccagta
    55861 aaaggatgcg tcaatgacaa ctgactggca tgcagcatta accgctgaag gccaaaatgc
    55921 tcagcaccgc tgcgattctg gcgtaaatca ccatgtttgc tgtcgccaat aatcgggtgc
    55981 cgcaaatggg caagatggcg acgaagctgg tgcttgcgtc cagttttcgg ctccagttcc
    56041 accaggccgt agcgcgtggt cgggtaacgt ccagtcgcta ccggcatttc tacggtcgcc
    56101 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    56161 gcgattttgt ccagttcttc caccagtgga taatccagca ccgcttcttc catcaaccag
    56221 ccacgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    56281 agccgtccgg cttcgctgga taatcccatc aacaacacac cagaagttgg tcggtccaga
    56341 cgatgagcag taaaaacatg ctggcctatc tggtcacgca cggtttgcat gaccactact
    56401 ttctcgtcgc gatccagcca gctgcggtga accagccagc cggagggttt atttaccgca
    56461 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    56521 catccagttt ttccagctct gccagaataa gcgcgcgttg cgggtagtcc gtcgccagcg
    56581 ccatttcata ataaggcgca acagcaaaag cgcccggtaa cggctggttg ttatctaaca
    56641 aatcgtgcat tcgcgggatc agcacccact gcaaccactc cagtggttcc atggtgtcca
    56701 taaagaacgg ttgagtactg ttaaattgat gcggcagggg ttcatcgttt cgccagtgct
    56761 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    56821 tcataaagca acctcaataa gaaaaactgc cgcgaagaat agcaggaata aaaaaaggga
    56881 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccggctactt ttattgctcc
    56941 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    57001 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgttcct ggccttcctg
    57061 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    57121 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    57181 ccttcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    57241 tagaatcgtc tttttctcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    57301 ttcatatgaa agttaagaat taacaagcaa catgttgttt ttaaatgaga ataacgattc
    57361 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    57421 ctcacaacca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    57481 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac
    57541 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    57601 tgggcggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    57661 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    57721 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aatcgtgggt tgtatcacaa
    57781 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    57841 agtatacggc atcttcggta gttgaaataa tgcacggtga aggaacgccg tacagttcct
    57901 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    57961 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    58021 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    58081 aggcacttgc gggcctgaca cttggaaaat caaccgatta ccgggatacc tatgacgcca
    58141 gcctactgca aggcgtacca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    58201 acctgccttt tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga
    58261 aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    58321 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    58381 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgcccaa ggtaaggtta
    58441 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    58501 cctgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    58561 agaatgccac cagcggtgaa aaagtagtgg aagagacgct tgtcagccac ctgctgaaat
    58621 caaattgcct gatcacccat caaccagatt ggggatcgat ccaaattcag tatcgtggac
    58681 gccaaattga cagagaaaaa ctgctgcgtt acctggtttc tttccgtcat cacaacgagt
    58741 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    58801 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    58861 gtaataacga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    58921 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcag caggcagggc tattgtaatc
    58981 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    59041 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    59101 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    59161 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc
    59221 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    59281 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgtgacatt ctcttcgttt
    59341 atgggcaaat tcataacacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    59401 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtc ggtgaagcgc
    59461 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    59521 gtcgcgtcgg gaaccagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccgg
    59581 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    59641 acagtcgctt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    59701 tggtcaacga attgatcatc atgccagata tcgaaaaacg tctggaagcg tttgtccgta
    59761 tcgctcacgg tatcattatc ttccctggcg gtgtgggtac agcagaagag ttgctgtatt
    59821 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    59881 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgtg catacgctgg
    59941 gtgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    60001 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg cgacactggt gacgcttaca
    60061 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    60121 agaatatggc taatctgaag ctttacccgg atcaacccgt tgaagtgctg gctgccgacc
    60181 tgcgccgtgc gttctccggt attgtggcgg gtaatgtaaa agaagtcggt attcgcgcca
    60241 ttgaaaagtt tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg
    60301 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    60361 cttgctacga aatctgcacg taatctcccc tcccccggtg acgtttgttg ccgggttttc
    60421 tcatttttgg tcacttactc atcaactcat ttcatttgtt atatgaatgt ttcttactac
    60481 cctcacgcga caaatatcac cacagttaat atgtcataca atttatgttg aaacgcaaac
    60541 gtttccctat tttcataaac catattttat tgccatgaaa aagaaattat cgccatgatt
    60601 gactaaaagt attgattttt tcagttcaac ctacatatat tgcgcgcccc gtaagaagtc
    60661 agatgtcgtt taatgggtaa atattgccct taaattctct tttacttttg atttacagag
    60721 taaagcgttg tgattatcta tcttccaagt agattatttt atttgggatc aagatcactg
    60781 atagatacat aacttgtgtg tatctttccg ccctcaaatt attacggtgg aaaatgatta
    60841 agccatcgcc gatagacaga tttcattttt acggtcaggc accttcccgg gctgaactgg
    60901 ctaaaagctg aattatttgc attcctccag gagaaataga tggaaacgac tcaaaccagc
    60961 acgattgcgt cgaaagactc tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg
    61021 ggcctttacg gcacggcaat cggcgcgggc gtgctgttcc tgccaatcaa cgctggtgtt
    61081 ggcggtatga tcccgctgat catcatggct atccttgcgt tcccgatgac gttttttgct
    61141 caccgcggcc tgactcgctt cgtactgtct ggtaaaaacc cgggcgaaga catcaccgag
    61201 gttgtagaag aacactttgg tattggcgca ggtaaactga ttactctgct ctacttcttc
    61261 gctatctacc cgatcctgct ggtttatagc gtggcaatca ccaatacagt tgaaagcttc
    61321 atgtctcacc agctgggtat gacgccaccg ccgcgtgcga ttctgtcgct gatcctgatc
    61381 gtgggtatga tgaccatcgt tcgcttcggt gagcagatga tcgttaaagc gatgagtatt
    61441 ctggtattcc cgtttgttgg cgtactgatg ctgctggctc tgtacctgat cccgcagtgg
    61501 aacggcgcag cactggaaac gctgtctctg gacactgcat ctgcaaccgg aaacggtctg
    61561 tggatgacac tgtggctggc aattccggta atggtgttct cgttcaacca ctctccgatc
    61621 atctcttctt tcgccgttgc gaagcgtgaa gagtacggcg atatggcaga acagaaatgc
    61681 tcgaagatcc tggcattcgc acacatcatg atggtgctga ccgtaatgtt cttcgtcttc
    61741 agctgcgtac tgagcctgac tccggcagac ctggctgcgg ctaaagagca gaacatctcg
    61801 attctgtctt acctggctaa ccactttaac gcgccgatca tcgcgtggat ggctccgatt
    61861 atcgcgatta tcgctatcac caaatccttc ctcggccact acctgggcgc acgtgaaggc
    61921 ttcaacggta tggtgattaa atctctgcgt ggtaaaggta agtctatcga aatcaacaag
    61981 ctgaaccgta tcactgcgct gttcatgctg gtaacgacct ggattgttgc caccctgaac
    62041 ccgagcatcc tgggtatgat tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc
    62101 ctgatgccga tgtacgcaat ccagaaagta ccggcaatgc gtaagtacag cggtcacatc
    62161 agcaacgtat tcgttgtcgt gatgggtctg attgcaatct ccgcaatctt ctactcactg
    62221 ttcagctaag tcctttcgcg ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta
    62281 tttcctatga ttagcgtatt cgatattttc aaaatcggca ttggcccttc cagttctcat
    62341 accgttggac caatgaaagc gggtaaacaa tttaccgacg atctgattgc ccgtaacctg
    62401 cttaaagacg tgacccgcgt ggtggttgac gtgtacggct cgctctctct gaccggtaaa
    62461 ggccaccaca cagatatcgc cataattatg ggtctggcgg gtaacctgcc ggataccgtg
    62521 gatatcgatt ccatccctgg ttttattcag gatgtgaata ctcatggtcg cctgatgctg
    62581 gcaaacggtc agcatgaagt ggagttcccg gttgatcagt gcatgaactt ccatgccgac
    62641 aacctttctc tgcatgaaaa cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt
    62701 tacagccaga cttattactc tatcggcggt ggctttattg ttgatgaaga acattttggt
    62761 cagcaggata gcgcaccggt tgaagttcct tatccgtaca gttcagcagc cgatctgcaa
    62821 aaacattgtc aggaaaccgg gctgtcactc tctggcctga tgatgaaaaa cgagctggcg
    62881 ctgcacagca aagaagagct ggaacagcac ctggctaacg tctgggaagt catgcgtggc
    62941 ggtattgagc gcggtatttc taccgaaggc gtgttacctg gcaaactgcg cgttccacgc
    63001 cgtgctgcag cactacgccg gatgctggtc agccaggata aaaccaccac tgacccgatg
    63061 gcggttgttg actggatcaa catgtttgca ctggcagtga acgaagagaa tgctgctggc
    63121 ggtcgcgtgg tgactgcgcc gactaacggt gcgtgcggga ttattccggc cgtgctggcg
    63181 tactacgaca agtttatccg cgaagtgaac gctaactcac tggctcgtta cctgctggta
    63241 gccagcgcca ttggttctct ttataagatg aacgcctcga tttctggtgc cgaagtcggc
    63301 tgccagggtg aagtcggcgt ggcgtgctca atggcggcag ctggtctggc agaactgtta
    63361 ggcgcaagcc cggcgcaggt atgcatcgcg gcggaaatcg ccatggagca caacctcggt
    63421 ctgacgtgtg acccggtcgc cgggcaggta caggtgccgt gcatcgagcg taacgccatt
    63481 gcggcagtga aagcagtaaa cgctgcacgt atggcgctgc gccgtaccag cgagccacgc
    63541 gtctgcctcg ataaagttat cgaaactatg tacgaaacag gtaaagatat gaacgccaag
    63601 taccgcgaaa cctctcgcgg cggcctggca atgaagatcg ttgcctgcga ttaatcgctc
    63661 tccagaggcc tctttttgcg aggcctcttc ccgatttctc atccagccat agcctgttct
    63721 ggcatcgaat gttaccctta tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg
    63781 cttattgtcg atgcactgaa tcttattcgt cgcattcatg ccgttcaggg ttcaccctgt
    63841 gtcgaaacct gccagcacgc gctcgatcag ctcattatgc acagccagcc aacccacgcg
    63901 gtcgccgttt ttgatgatga aaaccgcagt agcggctggc gtcatcagcg tttaccagat
    63961 tacaaagcgg gtcgaccgcc aatgccggaa gagttgcaca acgagatgcc tgcattacgc
    64021 gccgcctttg agcaacgcgg cgtcccgtgc tggtccgcca gcggcaacga agccgatgac
    64081 ttagccgcca cgctggcggt caaagtgaca caggccgggc atcaggcaac gattgtttcg
    64141 acagataaag gctactgtca gttactttca ccgacattac gtattcgtga ttacttccag
    64201 aaacgttggc tggatgcgcc atttatcgat aaagaatttg gcgttcaacc gcagcagttg
    64261 cccgattact gggggcttgc ggggatcagc agttcaaagg taccgggtgt tgcagggatc
    64321 ggaccaaaaa gcgccacaca gctactggtc gagtttcaga gtctggaagg gatatatgag
    64381 aatctggatg cggttgccga aaagtggcgc aaaaaattag aaacccataa agagatggcg
    64441 tttctgtgcc gcgatattgc ccgcttacaa accgatttac atatcgacgg caatttacag
    64501 caattgcggt tggtacgata aagagataca gggtgctacc gattaacaat ccattaaatt
    64561 taatgcactt gtaggccgga taagacgcgc cagcgccgca tccggcatta tcacatccgc
    64621 gcaattacca ggcggtatgg taaagctcta caatatcctc aagcgttgct tcacgcgggt
    64681 tgccaccggt acaaacatca tccagtgccg cctgcgccag tgccggaatg tcttccttgc
    64741 gtacaccaac atcacgtaaa tgtggcggaa taccgacatc acggttgagt gcaaacaccg
    64801 cttcaacagc ggcattacgc gcctcttcca ggctcatacc ttccactttc acgcccataa
    64861 cgcgcgcgat atcgcggtac ttctcaccgg taaagtcagc gttatagcgc atgacatgcg
    64921 gtaacaggat ggcgttcgca acaccgtgtg gcgtgttgta aaacgcgccc agtggatgcg
    64981 ccataccatg caccaaccct aatccaacat tcgagaatcc catacccgca acatactgcc
    65041 cgagcgccat ttcttctccg gcatccttat caccagcaac cgatcctcgc agcgccccag
    65101 caatgatttc aatcgcttta atgtgcagcg catcggttag cgcccacgcg ccacgggtaa
    65161 tatacccctc aatagcatga gtgagcgcat cgacacccgt cgcggctttc agcgctggag
    65221 gcataccatc catcatgtca gcgtcaataa acgccacctg cgggatatca tgcggatcaa
    65281 cgcaaacaaa cttgcgccgt ttctcttcgt cagtgatcac gtagttaatg gtcacttctg
    65341 ccgcagtgcc tgctgtggtg gggattgcca gaatcggtac actgggttta ttggtcgggg
    65401 aaagcccttc caggctacgc acatcggcaa actccgggtt gttgctgata atgccaatcg
    65461 ctttacacgt atcctgtgga gaaccaccac caatagcgat caggtaatcc gcgccgctat
    65521 tctggaatac atcgagccct tctttgacga cagtaattgt tgggttaggt actacgccgt
    65581 cgtaaatcgc ccatgccagt cctgccacat ccatcttatc gatcactttc gccaccacgc
    65641 cgcattgcac cagcgtttta tcggtgacga tcagcgcctt ctgataacca cggcgtttca
    65701 cctcatcggt taaagaccca acagcccccc gaccaaacca tgccgtttcg ttcagaatca
    65761 ttctgttagc catcatcctt ctccttgttg ctttacgaaa ttactcttca atccgtaacc
    65821 cgtaggtttt gaatttctcc agcactacgg caatctcttc atcgctcagc actggcaccg
    65881 ggtccgtaat cgccagggtc gtcaggtaaa gttgtgccag cacttcaact tcatgcgcca
    65941 gccataacgc tttctccaga ttcgcctcac aagcgataag cccatgatgt tgtaacaaag
    66001 ttgccttacg atttttgaga gccagcgcaa catgttcaga aagttcgcgt gttccaaagg
    66061 tcgcataagg cgcgcaagga atagaattac cgccagccgc cgcaatcatg tagtgaatcg
    66121 cggggatcgg tcggttaaga atggaaactg ccgtacaatg aacggcatga ttgtgaacaa
    66181 ccgcgttggc atccggtctg ctttgatagg ccgccatatg gaaacgccac tcgcttgagg
    66241 ggagttttcc ttcctcatgt ttgccgttgc catcaataaa gacaatatgc gactccgtca
    66301 gtttttcata tggaatacct gtaggcgtaa tcagcatccc atcctgataa cgtacactga
    66361 cgttacccgc tgtcccctgg ttcagcccca ggcgagtcat ttccaggcaa gtgtcaataa
    66421 tctgacgagc aagtttattt cgttccatta gctacctctc tctgattcaa aacagggcaa
    66481 taatgttgtt cctttcacac tattgaatta gccgtttaat tacccaccat cttcttcctg
    66541 attaaccaga aagaaattca caagcttata ttttgtgacc tggttcaact aatcacagta
    66601 aataactgca agttctcttt ttataactcc attaaaaatg accgctctta aaaatattta
    66661 tcaaaacggt catttttcta ctcctccctg ccaggaatgc ccgttttcgg cacaaacaat
    66721 taatacggtc atctggtttg tgttttttat gatttatttt ctgaaacggg catgaaattt
    66781 cgattattaa agtgatggta gtcacataaa gtcaccttcc agctaataag tgtgaccgcc
    66841 gtcatattac agaccgtttt ttatttgaaa atgaatccat gagttcattt cagacaggca
    66901 aatattcact gatatgaagc ccgaactcgc tggttttgca cttttgaaaa cataaccgat
    66961 tacgtgctta agcttctgaa cctaagagga tgctatggga aacacatcaa tacaaacgca
    67021 gagttaccgt gcggtagata aagatgcagg gcaaagcaga agttacatta ttccattcgc
    67081 gctgctgtgc tcactgtttt ttctttgggc ggtagccaat aaccttaacg acattttatt
    67141 gcctcaattc cagcaggctt ttacgctgac aaatttccag gctggcctga tccaatcggc
    67201 cttttacttt ggttatttca ttatcccaat ccctgccggg atattgatga aaaaactcag
    67261 ttataaagca gggattatta ccggattgtt tttatatgcc ttcggcgctg cattattctg
    67321 gcccgccgca gaaataatga actacacctt gtttttagtt ggcctattta ttattgcagc
    67381 cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt acggtattag ggccggaaag
    67441 cagtggtcac ttccgattaa atcttgcgca aacatttaac tcatttggcg caattatcgc
    67501 ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca catcaatcgc aagacgttct
    67561 cgataaaatg tctccagagc aattgagtgc gtataaacac agcctggtat tatcggtaca
    67621 gacaccttat atgatcatcg tggctatcgt gttactggtc gccctgctga tcatgctgac
    67681 gaaattcccg gcattgcaga gtgataatca cagtgacgcc aaacaaggat cgttctccgc
    67741 atcgctttct cgcctggcgc gtattcgcca ctggcgctgg gcagtattag cgcaattctg
    67801 ctacgtcggc gcacaaacag cctgctggag ctatttgatt cgctacgctg tagaagaaat
    67861 tccaggtatg acagcaggct ttgccgctaa ctatttaacc ggaaccatgg tgtgcttctt
    67921 tattggtcgt ttcaccggta cctggctcat cagtcgcttc gcaccacaca aagtcctggc
    67981 agcctacgca ttaatcgcta tggcactgtg cctgatctca gccttcgctg gcggtcatgt
    68041 gggtttaata gccctgactt tatgcagtgc ctttatgtcg attcagtacc caacaatctt
    68101 ctcgctgggc attaagaatc tcggccagga caccaaatac ggttcgtcct tcatcgttat
    68161 gaccattatt ggcggcggta ttgtcactcc ggtcatgggg tttgtcagtg acgcagcggg
    68221 caacatcccc actgccgaac tgatcccggc actctgcttc gcagtcatct ttatctttgc
    68281 ccgtttccgt tctcaaacgg caactaactg aacatatttt ccgaataaag tgaggaatca
    68341 gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc cggttattga cggtcgtcgc
    68401 atgggtgttc gtgagtcgct tgaagaacaa acaatgaata tggcgaaagc tacggccgca
    68461 ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg tcgagtgtgt catttccgat
    68521 acctgtatcg cgggtatggc tgaagccgct gcttgcgaag aaaaattcag cagtcagaat
    68581 gtaggcctca ccattacggt aacgccttgc tggtgctatg gcagtgaaac catcgacatg
    68641 gatccaaccc gcccgaaagc catttggggc tttaacggca ctgaacgccc cggcgctgtt
    68701 tacctggcag cggctctggc agctcacagc cagaaaggca tcccggcatt ctccatttac
    68761 ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg ctgatgttga agaaaaactg
    68821 ctgcgctttg cccgcgccgg tttagcagtc gccagcatga aaggtaaaag ctatctgtcg
    68881 ctgggcggcg tttcgatggg tatcgccggt tccatcgttg atcacaactt ctttgaatcc
    68941 tggctgggaa tgaaagtcca ggcggtggat atgaccgaac tgcgtcgccg tatcgatcag
    69001 aagatttacg acgaagccga actggaaatg gcgctggcct gggctgataa aaacttccgc
    69061 tatggcgaag atgaaaataa caaacagtat caacgcaatg ccgagcaaag ccgcgcagtt
    69121 ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca tgatgcaagg caacagcaaa
    69181 ctggccgata tcggtcgcgt ggaagaatca cttggctaca acgccatcgc tgcgggcttc
    69241 caggggcaac gccactggac cgatcaatat cccaatggcg acaccgccga agcgctcctt
    69301 aatagttcat ttgactggaa tggcgttcgc gaaccctttg tcgtggcgac cgaaaacgac
    69361 agtcttaacg gcgtggcaat gctaatgggt caccagctca ccggcaccgc tcaggtattt
    69421 gccgatgtgc gtacctactg gtcaccagaa gcaattgagc gtgtaacggg gcataaactg
    69481 gatggactgg cagaacacgg catcatccat ttgatcaact ccggttctgc tgcgctggac
    69541 ggttcctgta aacaacgcga cagcgaaggt aacccgacga tgaagccgca ctgggaaatc
    69601 tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat ggtgcccggc gatccacgaa
    69661 tacttccgtg gcggcggtta ctcttcccgc ttccttaccg aaggcggcgt cccgttcacc
    69721 atgactcgtg tcaacatcat caaaggcctg ggaccggtac tgcaaatcgc ggaaggctgg
    69781 agcgtggaat tgccgaagga tgtgcatgac atcctcaaca aacgcaccaa ctcaacctgg
    69841 ccaaccacct ggtttgcacc gcgcctcacc ggtaaagggc cgtttacgga tgtgtactcg
    69901 gtaatggcga actggggcgc taaccacgga gttctgacca tcggccacgt tggcgcagac
    69961 tttatcactc ttgcctccat gctgcgtatc ccggtatgta tgcacaacgt tgaagagacc
    70021 aaagtgtatc gtccttctgc ctgggccgcg cacggcatgg atattgaagg ccaggattac
    70081 cgcgcttgcc agaactacgg cccgttgtac aagcgttaat acattactcc ctgatgtgat
    70141 gcccggtcgc tccggctacc gggcctgaac aagcaagagt ggttagccgg ataagcaatg
    70201 ttatccggct atattgcagg agcgattatg aaacaagaag ttatcctggt actcgactgt
    70261 ggcgcgacca atgtcagggc catcgcggtt aatcggcagg gaaaaattgt tgcccgcgcc
    70321 tcaacgccta atgccagcga tatcgcgatg gaaaacaaca cctggcacca gtggtcttta
    70381 gacgccattt tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgat
    70441 tgccacatcc gcggtatcgc cgtcaccacc tttggtgtgg atggcgctct ggtagataag
    70501 caaggcaatc tgctctatcc gatcattagc tggaaatgtc cgcgaacagc cgcggtaatg
    70561 gacaatattg aacggttgat ctccgcacag cagttgcagg ctatttctgg agtcggagcc
    70621 tttagtttca atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg
    70681 gaacgcgcgc acgcctggct ctttatttcg tcgctgatta accaccgttt aaccggcgaa
    70741 ttcactactg atattacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat
    70801 ttcagtccgc aaattttaca agccaccggt attccacgcc gactcttccc tcgtctggtg
    70861 gaagcgggtg aacagattgg tacgctacag aacagcgccg cagcaatgct cggcttaccc
    70921 gttggcatac cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgctggt
    70981 gccgaacaaa atgaacccgt gctctcttcc ggtacatggg aaattttaat ggtccgcagc
    71041 gcccaggttg atacttcgct gttaagtcag tacgccggtt ctacctgcga actggatagc
    71101 caggcagggt tgtataaccc aggtatgcaa tggctggcat ccggcgtgct ggaatgggtg
    71161 agaaaactgt tctggacggc tgaaactccc tggcaaatat tgattgaaga agctcgcctg
    71221 atcgcgcctg gcgcagatgg agtgaaaatg cagtgtgatt tattgtcgtg ccagaacgct
    71281 ggctggcaag gcgtaacgct taataccacg cgggggcatt tctatcgcgc ggcgctggaa
    71341 gggttaacca cgcaattaca gcgcaatcta cagatgctgg aaaaaatcgg gcactttaaa
    71401 gcctctgaat tattgttagt cggcggagga agtcgcaaca cattgtggaa tcagattaaa
    71461 gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg ccgaaacgac cgtcgcagga
    71521 gctgcgctgt ttggttggta tggcgtaggg gaatttaaca gcccggaaga agcccgtgca
    71581 cagattcatt atcagtaccg ttatttctac ccgcaaactg aacctgaatt tatagaggag
    71641 gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc gaactattga aagtgctggc
    71701 agagatgggg catggagatg aaattatttt ttccgatgct cactttcccg cccattcgat
    71761 gggaccgcag gtgatccgcg ctgatggcct gttggtgagc gacttgctcc aggcgattat
    71821 cccgttattt gagctggaca gttatgcacc accgctggtg atgatggcgg cggtagaagg
    71881 tgacactctc gatcctgaag tagaacgacg ttatcgtaat gcgctttcac tacaagcccc
    71941 ctgtcctgac atcatccgca tcaatcgttt tgcgttttat gaacgggcgc aaaaagcctt
    72001 tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat attcttttaa aaaaaggggt
    72061 aacaccgtaa tctcataccg gtacgcccgc atgacgcggg cggttatcga atgatggggt
    72121 gaaaaatatg aaagcggcac gccagcaagc gatagtcgac ctgctgctga accataccag
    72181 cctgaccacg gaagctctct ctgaacagct aaaggtcagt aaagaaacca ttcgtcgcga
    72241 tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat catgggcgcg ctaaatatat
    72301 ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc cggctgaaaa gccattatgc
    72361 gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt gaagaaggga tggtgatagc
    72421 cttagacgcc agttcaacct gctggtatct ggcacgccag ttgcctgaca tcaacatcca
    72481 ggtcttcacc aatagccatc cgatttgcca tgaactcggt aaacgcgaac gcattaaact
    72541 gatcagttcc ggcggcacac ttgagcgcaa atatggctgt tacgtcaatc cctcgctgat
    72601 ttcccaactt aaatcgctgg aaatcgatct gtttattttt tcttgtgaag ggatcgatag
    72661 cagcggcgca ctgtgggact ccaatgccat caacgctgat tacaaatcaa tgttattaaa
    72721 acgtgccgcg caatcgttgt tattgattga taaaagtaaa tttaatcgtt caggggaagc
    72781 ccgcatcggg catctggatg aggtaacgca cattatttct gatgagcgcc aggttacaac
    72841 ttctttggta acagcctgac ggaaagggta gcaggccgga gacgacgccc cggccttacc
    72901 tgttatcgct cgtcgcgacg accacccacc gcagcccaga tacggcggac gtgcaccgtc
    72961 acttcttcgc gatcgtgata caactgccgc gcctgaatct gagcatttat gccatgttca
    73021 tcaagctgtg cctgaatata cgccagattg tgtgacactt cttcgtagcg ttttttcatc
    73081 ggcagtttga gattgaagat agtttcacgg caccagccat taaccagcca ctgcgccatt
    73141 aatgccgcaa ctttcgccgg tttttcaacc atatcgcata ccatccagga gatattgctg
    73201 cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg tcacctgccc ggtatccatc
    73261 agactttgcg ccatcgggcc gttgtcgacg gaataaaccc acatgttgcg cttcaccagt
    73321 tggtaggtcc agccgcctgg gcaagcacct aaatccaccg cccacatccc gttcgccagg
    73381 cgttcatccc actcatccgc gggaataaac acatgaaatg cctcttccag tttgagcgtg
    73441 gaacgactcg gcgcatctgc cggaaatttc aggcgcggaa tgcccatata gaacggcgaa
    73501 ttattgctgc tgtatgagta accagtatag caacagcctg gtgcaataaa gaatacatgc
    73561 acaaccggac gcttcggcgt ttcatagttc gccagcaccc ccgcatcacg caaggcagcg
    73621 cgtagcggca cggtgaattt acggcagaat ttcagcaact ctttgctttc gttggtatcg
    73681 gcaacttcaa cacgcagttc accgcccttc tctactacgc cctgtaacat gccgacaatg
    73741 ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt cccccactac aaaccactgg
    73801 cgggcaaaaa ttaatgaact gaacggcagc tcacggatta acttatcgcc atcatcaggt
    73861 tgataacatt cataaatgac ataacccgca ttctctttca cgcgggcaaa accgaaaatt
    73921 tcccgctggc cggctttatc ggtaatttct gcggcgcact ctttttcaaa gcccggacgg
    73981 cacagcaata caaccttatt catgacttac gcccttgcgc tttaaacgga tagcaccaac
    74041 taacattaac gcccagcccg caaggaagct cacgccgcca accggtgtga caaacgccca
    74101 caaacgcaga tgggacagcg ccaggcaata aaggctgccg ctgaacagca ccgtgcctaa
    74161 cgcgaggaaa acgctactcc agtaaaacca gatactgatg cgacgctgca ttgccaccgc
    74221 cagacctaag atcgccagcg tatgaaacgc ctggtattcg aggccggtct ggatccagcc
    74281 catctcaacg gcccccatgg ttttacttaa cacatgcgcg ccaaaagcgc ccagagctac
    74341 aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa cggctggtca tggtcgtacc
    74401 ctacgtaaat tattgttcat aacgaaagcg gaatttttct tgttcagcag cggctttcgc
    74461 caggatccat tggcgaaagg cggctatttt acccagttct gcctggctgt catgacaaac
    74521 cagataaaaa gcgtttttac tgaccagaac atcattaaac gggcaaacaa gacgtccggc
    74581 ctcgatttca gattgcgcca tcacgttatt tgcaagcgcc actccctgcc cgtggatagc
    74641 cgcttgcagc accatggcgc tatggctaaa aattggccct tgctgaacgt tgatatgatt
    74701 taaccccaac tgtcgggtat atgtctgcca gtcacggcgt gacgcatcat gtaataacgt
    74761 atgtttagcc agatcttccg gggtcttcaa gggtttttcg ccagtcagca gtagcggcga
    74821 acacaccggc aataaatatt cggcgtacag tttttccacc cgtagccccg gccagttgcc
    74881 ccgaccataa aatatcgcca catcaacatc atccgccagc ttatcttcct gacgatcaac
    74941 cgcctggatt cgaacgtcaa ttcccggata agctgaatta aagctggaaa gtcgcggaac
    75001 caaccaatga atcgcgaaac tgggaagtaa actgaccgtc aacgccccct tagcgctacg
    75061 ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat atctctttga tatcgaggaa
    75121 atagctttgc ccttcctcgg tcagcaggag tgaacgatta cggcggcgga acagttttag
    75181 ccccagaaaa tcctcaagag acttgatttg atgacttact gcggcctggg tcacaaaaag
    75241 ctcttctgct gcgcgagtga aacttaaatg gcgtgctgcg gcatcaaaaa ctcgtaaggc
    75301 atttagcggt ggtaatcgtt tagacatggc ttttaaactt tgatgttaaa tgaatttaac
    75361 aattagatta cactgtgtaa cctattagtt tttttaatct gagccattat aaattgtccg
    75421 ttgagcttct accagcaaat acctatagtg gcggcacttc ctgagccgga acgaaaagtt
    75481 ttatcggaat gcgtgttctg gtgaactttt ggcttacggt tgtgatgttg tgttgttgtg
    75541 tttgcaattg gtctgcgatt cagaccatgg tagcaaagct accttttttc acttcctgta
    75601 catttaccct gtctgtccat agtgattaat gtagcaccgc ctaattgcgg tgcttttttt
    75661 taccttgcga tcgcgaatta ctgatccagt tcgaccatct ctttcacgtc agtacggttg
    75721 atctgctgtt tgttgccatt agcgtcttta tacgaaatca taccggtatc gttatcagtc
    75781 tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg tgtgcatcac atagttcgaa
    75841 ccggaacagg cgctcagggc aaaagtcagc atacaggcag aaataattgc ggcagtcttt
    75901 ttcatcatct tctccttaaa gcgattaata aatcttaata gtattgatta acaggctaaa
    75961 attaacgcct aacactattc agcatatgtt actgacgcgg cttcgccagg atatccagat
    76021 aattctgatg gttagcactc tccttgtatc aaagtgaatt ttgcgtcacg atcggtgcat
    76081 caagccgagg agtaccatga acgtttttaa tcccgcgcag tttcgcgccc agtttcccgc
    76141 gctacaggat gcgggcgtct atctcgacag cgccgcgacc gcgcttaaac ctgaagccgt
    76201 ggttgaagct acccgacagt tttatagcct gagcgccgga aacgtccatc gcagccagtt
    76261 tgccgaagcc caacgcctga ccgcgcgtta cgaagctgcg cgggaaaaag tggcacaatt
    76321 actgaatgca tcggatgaca aaaatatcgt ctggacgcgc ggcaccactg aatccatcaa
    76381 catggtggcg caatgctatg cccgtccgcg tctgcaaccg ggcgatgaaa ttattgtcag
    76441 tgtggcagaa caccacgcca acctcgtccc ctggctgatg gtcgcccaac aaacgggggc
    76501 caaagtggtg aaattgccgc ttaatgcgca gcgactgccg gatgtcgatt tgttgccaga
    76561 actgattact ccccgtagtc ggattctggc gttgggccag atgtcgaacg tcactggcgg
    76621 ctgcccggat ctggcgagag cgattacttt tgctcattca gccgggatgg tggttatggt
    76681 tgatggtgct cagggggcgg tgcatttccc cgcggatgtt cagcaactgg atattgattt
    76741 ctatgctttt tcaggtcaca aactgtatgg cccgacaggc atcggcgtgc tgtatggcaa
    76801 accagaactg ctggaagcga tgtcgccctg gctgggcggc ggcaaaatgg ttcacgaagt
    76861 gagttttgac ggcttcacga ctcaatctgc gccgtggaaa ctggaagcag gaacgccaaa
    76921 tgtcgctggc gtcataggat taagcgcggc gctggaatgg ctgacagatt acgatatcaa
    76981 ccaggccgaa agctggagcc gtagcttagc aacgcttgca gaagaagcgc tggcgaaacg
    77041 tccaggcttt cgttcattcc gctgccagga ttccagcctg ctggcctttg attttgcggg
    77101 tgttcatcac agcgatatgg tgacactgct ggcggagtac ggtattgccc tgcgcgccgg
    77161 gcagcattgc gctcagccgc tactggcaga attaggcgta accggcacac tgcgcgcctc
    77221 atttgcgcca tataatacaa agagtgatgt ggatgcgctg gtgaatgccg ttgaccgcgc
    77281 gctggaatta ttggtggatt aatgacaaac ccgcaattcg ccggacatcc gttcggcaca
    77341 accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt tgacgcaatg ggaagataaa
    77401 tatcgccagt tgatcatgct ggggaaacag cttccggctt tgccagagga gttaaaagcg
    77461 caggctaaag agattgccgg atgcgaaaac cgcgtctggc tgggatatac cgtggctgaa
    77521 aacggcaaga tgcatttctt tggcgacagc gaagggcgca ttgtgcgcgg cctgttggcg
    77581 gtgttgttga ctgccgttga ggggaaaacc accgccgaat tgcaggcaca gtcgccgctg
    77641 gcattgtttg atgagctggg attacgtgcg caacttagcg cctcacgcag ccaggggttg
    77701 aatgcgttaa gcgaggcgat tatcgctgcg gcgaagcagg tttaagcaca gaaccaggtc
    77761 ggataagacg ttcgcgtcgc atccgacctg attgtttacc cctgacgcgc cgctttcgcc
    77821 atcatcttct tcagcgcatg agaaaccgca acaaagccaa aagtggcggt caccatcgtc
    77881 gccgcgccaa atcctgaggc acaatccatc cgctttggcc cttccgccgt ggctttcatc
    77941 gcacagacgg tgccgtctga ctgcgggtac accagcgctt cggtagagaa cacgcaatcc
    78001 acgccgagct tacctttact gtttttcact acgccaaaat cgcttttcag gcgctcgcgc
    78061 aacttcgccg ccaacgggtc ctgaatcgtt ttcgccagat cggtaacctg aatctgcgtc
    78121 gggtcaatct gcccaccagc gccaccggtc gtgaccagcg ggattttatt gcgccgacaa
    78181 taagcaatca gcgccgcttt gggccgtaca ctatcaatag catcaatcac gtacgaataa
    78241 ccggcattca tatattgcgc tacgttatcc ggcgtcacga agtcatccac caccattaca
    78301 cggcactccg ggttaatctg gcgaatacgc tccgccatga cttccgcttt tgccagcccg
    78361 acgttatcgc gcagagcgtg aatttgccga ttggtattgg tgacgcacac atcatccata
    78421 tcgataagcg tgattgcgcc aatccccgtg cgcgctagcg cttccgccgc ccaggaaccg
    78481 acaccaccga taccaaccac acaaatatgc gcgtcagcaa acagttgcaa tgctttttca
    78541 ccatacagac gcgctgtgcc accaaaacgc tgacgccatg catcactaat taccacagac
    78601 atagcacctc agaattaaaa agggtgaggt tacccctcac cctgtcatat ccgtaaaaac
    78661 ggcatacaga atatcacatc agccgctaaa gacgttacct gcgcccgggg cggttttcag
    78721 cacccagaca cgtccatagt ggttgtacca acctgcgcgg tgtccggctt ccggcccgat
    78781 cccttgatag atatcgaagt gttggccttt gattgcgcca ccgacatcca gcgccaccat
    78841 cagacgcaat tcgtactgac cgttaaattt gccgttatta tccagcagcg gcacttctgc
    78901 cagcaaggta gtacctggcg gaataattga acgatcggag gcaacagaag cacggccaac
    78961 cagcggcacc gcacttgccc ctttcaccgg tgcaaaagat tgcggtttaa agaagacgaa
    79021 agacgggttc tgttccagca gctcgcgaac ctccgcttca ctgtgtgttt cgccccagtg
    79081 acgaatcgcc tgcatcgaca tatcttcttt tttcacttcg ccacggtcga tcaacacctt
    79141 accaatgctg cgataggcat gaccgttttt ccctgcatag ctgaaaaagt taagcggact
    79201 gccatcacca aagtcgatat acccgctacc ctgcacatcc ataatgaagt tatccatcag
    79261 ggagttactg taagcgagaa tatatttatc actcagcgct cctgcgtaga tctccgcacg
    79321 agacggcaga cgaccacgtt ttggcggcat acgataaatc ggatactgga actcgccctg
    79381 acgggtatgg cgcgcctgaa ttaccggcgt gtaatagcct gtaaactgca cgttaccata
    79441 gttgtcgaca ccttccatct gccaggcatc aatgccgaac tggcgcatat tgcgggtatc
    79501 accgcctgcg cgcagccact cttgcaccgc gttataaaca ttactctggt tgccatacag
    79561 acgcggtgac gaattacgga tatggttaat ttgctcggca aaatcaccgg cattaatcgg
    79621 cgcgccaacg gcatctggct ggttcaccag agagaaaggc tgggtaaatt tcccgtcttt
    79681 atattgctgt ccgcgatcgg ttggtttgga agagcaggcg gcaagcattg ccacaaccgt
    79741 gcccataaga aggtacttta cccaacgtcc tttcattgtt ctcttcttca ggttaaaaaa
    79801 taaggcgcaa cgaagataac aaaccaccgc gtttaatgaa atgagcagcc tctccctgac
    79861 gcaaattttg cacaaaaaac aggctttagt gatttgtttt tgttcaaaat catgccaaat
    79921 ccgtgatcgg ggtaaaaaaa aggttgcatg aaaacgcgag cagagtatag tgcgcatcca
    79981 cggacgcggg gtggagcagc ctggtagctc gt
//
LOCUS       DAZFRE010000011        75308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFRE010000011 DAZFRE010000000
VERSION     DAZFRE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.011"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(40..1299)
                     /gene="rhaA"
                     /locus_tag="ACHKUI_000871"
     CDS             complement(40..1299)
                     /gene="rhaA"
                     /locus_tag="ACHKUI_000871"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6597128.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            complement(1296..2765)
                     /gene="rhaB"
                     /locus_tag="ACHKUI_000872"
     CDS             complement(1296..2765)
                     /gene="rhaB"
                     /locus_tag="ACHKUI_000872"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6597129.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            3053..3889
                     /gene="rhaS"
                     /locus_tag="ACHKUI_000873"
     CDS             3053..3889
                     /gene="rhaS"
                     /locus_tag="ACHKUI_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO6597130.1"
                     /translation="MTVLYSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPLR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            3963..4811
                     /gene="rhaR"
                     /locus_tag="ACHKUI_000874"
     CDS             3963..4811
                     /gene="rhaR"
                     /locus_tag="ACHKUI_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6597131.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4808..5842)
                     /gene="rhaT"
                     /locus_tag="ACHKUI_000875"
     CDS             complement(4808..5842)
                     /gene="rhaT"
                     /locus_tag="ACHKUI_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO6597132.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            6128..6748
                     /gene="sodA"
                     /locus_tag="ACHKUI_000876"
     CDS             6128..6748
                     /gene="sodA"
                     /locus_tag="ACHKUI_000876"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6597133.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFTAKK"
     gene            7008..7990
                     /gene="kdgT"
                     /locus_tag="ACHKUI_000877"
                     /pseudo
     CDS             7008..7990
                     /gene="kdgT"
                     /locus_tag="ACHKUI_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            8139..8813
                     /gene="yiiM"
                     /locus_tag="ACHKUI_000878"
     CDS             8139..8813
                     /gene="yiiM"
                     /locus_tag="ACHKUI_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6597134.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGVVSADAPL
                     ELVSRVSDMTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(8985..10358)
                     /gene="cpxA"
                     /locus_tag="ACHKUI_000879"
     CDS             complement(8985..10358)
                     /gene="cpxA"
                     /locus_tag="ACHKUI_000879"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6597135.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10355..11053)
                     /gene="cpxR"
                     /locus_tag="ACHKUI_000880"
     CDS             complement(10355..11053)
                     /gene="cpxR"
                     /locus_tag="ACHKUI_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6597136.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            11203..11703
                     /gene="cpxP"
                     /locus_tag="ACHKUI_000881"
     CDS             11203..11703
                     /gene="cpxP"
                     /locus_tag="ACHKUI_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6597137.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            complement(11890..12870)
                     /locus_tag="ACHKUI_000882"
     CDS             complement(11890..12870)
                     /locus_tag="ACHKUI_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6597138.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSIRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(12940..13233)
                     /locus_tag="ACHKUI_000883"
     CDS             complement(12940..13233)
                     /locus_tag="ACHKUI_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6597139.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            13370..13642
                     /locus_tag="ACHKUI_000884"
     CDS             13370..13642
                     /locus_tag="ACHKUI_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO6597140.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKLKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            13645..13815
                     /locus_tag="ACHKUI_000885"
     CDS             13645..13815
                     /locus_tag="ACHKUI_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597141.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            13812..14312
                     /locus_tag="ACHKUI_000886"
     CDS             13812..14312
                     /locus_tag="ACHKUI_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO6597142.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            14376..14600
                     /locus_tag="ACHKUI_000887"
     CDS             14376..14600
                     /locus_tag="ACHKUI_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO6597143.1"
                     /translation="MHTVSENQCGIYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQVSEKFHNIGLSRGEVL"
     gene            14600..14899
                     /locus_tag="ACHKUI_000888"
     CDS             14600..14899
                     /locus_tag="ACHKUI_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO6597144.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            14902..15126
                     /locus_tag="ACHKUI_000889"
     CDS             14902..15126
                     /locus_tag="ACHKUI_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6597145.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDTPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            15123..15398
                     /locus_tag="ACHKUI_000890"
     CDS             15123..15398
                     /locus_tag="ACHKUI_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO6597146.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            15388..17673
                     /locus_tag="ACHKUI_000891"
     CDS             15388..17673
                     /locus_tag="ACHKUI_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6597147.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFATRISSQLFIMYEELCDAWVDA
                     HGEKESLFTDEAQDHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTTPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGRRTPNRQ
                     QRNGSPLKPYEMAPSARLTRSERMQITRIRVDLAQKGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMEWK"
     gene            17673..18122
                     /locus_tag="ACHKUI_000892"
     CDS             17673..18122
                     /locus_tag="ACHKUI_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFO6597148.1"
                     /translation="MAKIHEVKLHAKYFDLVLEGKKRAEFRKNDRNYERGDTLILHEW
                     VQGVFTGRKVEARITDVTDLSDWLEDYVLLSIELLNTGAYEIVNWKELSERGLVFRIN
                     HEIMHQLGLAVMYESVTGLSGGAVVAADGMWSYSDEQVECAKQKGWF"
     gene            18125..18358
                     /locus_tag="ACHKUI_000893"
     CDS             18125..18358
                     /locus_tag="ACHKUI_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597149.1"
                     /translation="MHRIPGEIPQHKTKNIKLMAIVHRLQQIMVNENLTPDELVGCAE
                     MVRDNYRRFNDISRQKIEVFRPANMPKKLPHRM"
     gene            18610..19548
                     /locus_tag="ACHKUI_000894"
     CDS             18610..19548
                     /locus_tag="ACHKUI_000894"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6597150.1"
                     /translation="MPTVVSLFSGCGGSDAGVLRAGFNVLMANDILPYARDVYLENHP
                     ETDYILGDISGLQSFPSAELLIGCYPCQGFSQGGARKADRKINTLYLEFARALRKIKP
                     KAFIVENVSGMVRRNFEHLLKDQFKVFEEAGYTVSSQILNASHYGVSQDRKRIFIVGI
                     RKDYGITYKFPKPTHGDGLKPYSTIRDAIGHMPVWPIGEFYDADFHWYYLSRNRRQDW
                     DQISKTIVANPRHMPLHPISPTLEKLGPDKWQFTSDAPARRFSYREAAILQGFGDLIF
                     PETERASINMKYTVVGNAVPPPLFEAVAKSLPDIWD"
     gene            complement(19549..20541)
                     /locus_tag="ACHKUI_000895"
     CDS             complement(19549..20541)
                     /locus_tag="ACHKUI_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597151.1"
                     /translation="MKIFKIGDIHPSVPHLFADLAELATVINYSGRYDLHKNDLITIR
                     TQSNTSVDDIDQEYQEDENEGCDAERNDRLERQVEDVWTQLDFRQNFLKDIYPFIING
                     DFISLKENLTEIQRIYIFLLACSRLRSFTKARQGIIQFWAKNFAVVSKFCATALLPAH
                     STVRVFDANSDDRRKYYGTDLRKALKRLGKDLAVPYINEQECERADPRGDGGFDIVAT
                     IEFEDKLSSNFAFLGQCGAQERDWPKKTLEAHSLKLRTYFQVHFDIPTLMFTPVFYRN
                     SDGQWVDNSPCAGVLIIDRARILQLLKKTNHCPKIVSEQWFLDFEQIINDLKVE"
     gene            complement(20528..21646)
                     /locus_tag="ACHKUI_000896"
     CDS             complement(20528..21646)
                     /locus_tag="ACHKUI_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6597152.1"
                     /translation="MTESLDYIDLNKLELDTKNPRLPEGVERTPEAMLNHIALTTSIE
                     DLMNAIAENGFFPGEPLIAVKEGDKYTVVEGNRRLTAVKLIHNPYECDRPSSRMIEIA
                     ESAKDKLGTLEKLPVIVRDTRAEILPYLGFRHITGVKQWEPLSKARYIEQLFGLTSPN
                     SPTNDRYHQVARAIGSRKDHIKRNLDALAVYKVMESNNFYDIDGLDEESIKFSILSTA
                     LADEKIGLFVGVSEKDEYGDITSNDVIIHPHHINRENTRELTVWLYKKDDSGKTKVGE
                     SRNLRLLSSVIDNPKALTSFRNGADLKVAYQLTEDLKQDFMTLLYKAESALIEAAGIV
                     ATIDYNPEALEVARRLSQNVKLIGNTIKAKKVSDDEDF"
     gene            complement(22022..23056)
                     /locus_tag="ACHKUI_000897"
     CDS             complement(22022..23056)
                     /locus_tag="ACHKUI_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6597153.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWIGQEVIRF
                     KNYSLDTDNG"
     gene            complement(23056..24828)
                     /locus_tag="ACHKUI_000898"
     CDS             complement(23056..24828)
                     /locus_tag="ACHKUI_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO6597154.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LCSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            25002..25856
                     /locus_tag="ACHKUI_000899"
     CDS             25002..25856
                     /locus_tag="ACHKUI_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6597155.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            25915..26988
                     /locus_tag="ACHKUI_000900"
     CDS             25915..26988
                     /locus_tag="ACHKUI_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6597156.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATEEPGA"
     gene            26992..27735
                     /locus_tag="ACHKUI_000901"
     CDS             26992..27735
                     /locus_tag="ACHKUI_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO6597157.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVNPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAVTRAPRKTRSVTPAKRGRPKKKAS"
     gene            27835..28344
                     /locus_tag="ACHKUI_000902"
     CDS             27835..28344
                     /locus_tag="ACHKUI_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6597158.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTCLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            28344..28547
                     /locus_tag="ACHKUI_000903"
     CDS             28344..28547
                     /locus_tag="ACHKUI_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6597159.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGVVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            28551..28832
                     /locus_tag="ACHKUI_000904"
     CDS             28551..28832
                     /locus_tag="ACHKUI_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6597160.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            28832..29329
                     /locus_tag="ACHKUI_000905"
     CDS             28832..29329
                     /locus_tag="ACHKUI_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO6597161.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKMFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            29344..29769
                     /locus_tag="ACHKUI_000906"
     CDS             29344..29769
                     /locus_tag="ACHKUI_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO6597162.1"
                     /translation="MKKLSLSLMLNVSLALMPALSLIYPQSVAVSFVATWAILAMVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLICTACRQRLEEREPCQS"
     gene            29757..30182
                     /gene="lysB"
                     /locus_tag="ACHKUI_000907"
     CDS             29757..30182
                     /gene="lysB"
                     /locus_tag="ACHKUI_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO6597163.1"
                     /translation="MSKLMIVLVVLLSLAVSGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            30082..30327
                     /gene="lysC"
                     /locus_tag="ACHKUI_000908"
     CDS             30082..30327
                     /gene="lysC"
                     /locus_tag="ACHKUI_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO6597164.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            30290..30757
                     /locus_tag="ACHKUI_000909"
     CDS             30290..30757
                     /locus_tag="ACHKUI_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6597165.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            30750..31202
                     /locus_tag="ACHKUI_000910"
     CDS             30750..31202
                     /locus_tag="ACHKUI_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6597166.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            31269..31904
                     /locus_tag="ACHKUI_000911"
     CDS             31269..31904
                     /locus_tag="ACHKUI_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6597167.1"
                     /translation="MNTLANIQELARALRNMIRPGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            31901..32248
                     /locus_tag="ACHKUI_000912"
     CDS             31901..32248
                     /locus_tag="ACHKUI_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6597168.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            32253..33161
                     /locus_tag="ACHKUI_000913"
     CDS             32253..33161
                     /locus_tag="ACHKUI_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO6597169.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            33154..33684
                     /locus_tag="ACHKUI_000914"
     CDS             33154..33684
                     /locus_tag="ACHKUI_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6597170.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTVGAIRRVVEPLGYLINVKE
                     WWETNDPPGTFRLDIGVLESGITEEMYLEMERLIADAKPASRHLIGLNIIQDIPGYLY
                     TGGVVCDGDVITVYPG"
     gene            33695..36436
                     /locus_tag="ACHKUI_000915"
     CDS             33695..36436
                     /locus_tag="ACHKUI_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6597171.1"
                     /translation="MSTKFKTVITTAGAAKLAAATVPGGKKVNLSAMAVGDGNGKLPV
                     PDAGQTKLVHEVWRHALNKVSVDNKNKNYIVAELVVPPEVGGFWMRELGLYDDAGTLI
                     AVSNMAESYKPELAEGSGRAQTCRMVIILSNVASVELSIDASTVMATQDYVDDKIAEH
                     EQSRRHPDATLTEKGFTQLSSATNSTSEKLAATPKAVKAANDNANSRLAKNQNGADIQ
                     DKSAFLDNIGVTSLTFMKHNGMIPTTDNLDSYGPEEKYLGTWSCPSQSTAKPESGYPE
                     DKGNGVLEVFNAGRFHCTQRYTTRTGNIYIRMLDAEWNPASPTWSAWRVITSGTRPLS
                     TSIDLNSLGGAEHLGIWRNSSTSIASFERHFPEDGSFGQGILEVFEGGLYGRMQRYTT
                     RSGTMYIRGLTASWDAENPQWEDWIAVGYQSTGWTYSGDLDDLLKPGIYSVTKQATNA
                     PVTDSKDLAVGSIVEVKKRCDIESYIQTYTTVSATDAYKNRTFQRTRASGEADWGEWA
                     EVYNSKSLLTKLGVGGVTDRLSSLDWQTYDFVPGSMITVRLSDMTNIPDGMEWGVIDT
                     NLINITVGPSEGGGVARSMQVWRSTSNKTNYRFFTVRLYGNPGERSFNIRRLPIIDEA
                     QTWEAKQTFSAGLSGELSGNAATATKLKTARKINNVSFDGTSDINLTPKNIGAFASGK
                     TGDTVANDKAVGWNWSSGAYNATTGGASTLILHFNIGEGSCPAAQFRVNYKNGGIFYR
                     SARDGYGFEADWSEFYTTTRKPTAGDVGALSLSGGQLNGALGIGTSSALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANSVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDV
                     RLGTRVVQTMQKGVMYEKSGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            36440..36967
                     /locus_tag="ACHKUI_000916"
     CDS             36440..36967
                     /locus_tag="ACHKUI_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6597172.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTFNPEGFSVVEVPDITANRRADDSGKWMFKDGAVI
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(37119..37898)
                     /locus_tag="ACHKUI_000917"
     CDS             complement(37119..37898)
                     /locus_tag="ACHKUI_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597173.1"
                     /translation="MSNETQSFLSQLLISIISISLGSFLFAGILESYKKDQDLQEEFI
                     KDYFRPMMKLQSSCSSSHNELFLKYGELSASYQLMYNEIVHMAMSPDSELGQHYEVLP
                     MSLIKTNEELKKRVEDLEMTVKKCNIDLFLKYEELALVTGSYPEFKRLAKKHTNTINS
                     IYSERQKKAKENAKNIDPDQLIPLMRKYIAMDPHTNVNKSMLASEIDNISKLTTQHNL
                     IMAEYEELIFKEDNDLFITLHDLYAIKISEKYSGGFISWIF"
     gene            38299..39489
                     /locus_tag="ACHKUI_000918"
     CDS             38299..39489
                     /locus_tag="ACHKUI_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO6597174.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            39502..40020
                     /locus_tag="ACHKUI_000919"
     CDS             39502..40020
                     /locus_tag="ACHKUI_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6597175.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            40078..40353
                     /locus_tag="ACHKUI_000920"
     CDS             40078..40353
                     /locus_tag="ACHKUI_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6597176.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            40350..40505
                     /locus_tag="ACHKUI_000921"
     CDS             40350..40505
                     /locus_tag="ACHKUI_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6597177.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            40498..42945
                     /locus_tag="ACHKUI_000922"
     CDS             40498..42945
                     /locus_tag="ACHKUI_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6597178.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            42960..43439
                     /locus_tag="ACHKUI_000923"
     CDS             42960..43439
                     /locus_tag="ACHKUI_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6597179.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMETRFTGMTGAG"
     gene            43439..44602
                     /locus_tag="ACHKUI_000924"
     CDS             43439..44602
                     /locus_tag="ACHKUI_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6597180.1"
                     /translation="MLDTLTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKNPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDD"
     gene            44683..44901
                     /gene="ogrK"
                     /locus_tag="ACHKUI_000925"
     CDS             44683..44901
                     /gene="ogrK"
                     /locus_tag="ACHKUI_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO6597181.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVGPHPLPSGQQIMWM"
     gene            45137..46039
                     /gene="fieF"
                     /locus_tag="ACHKUI_000926"
     CDS             45137..46039
                     /gene="fieF"
                     /locus_tag="ACHKUI_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6597182.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            46220..47182
                     /gene="pfkA"
                     /locus_tag="ACHKUI_000927"
     CDS             46220..47182
                     /gene="pfkA"
                     /locus_tag="ACHKUI_000927"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6597183.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            47502..48491
                     /gene="sbp"
                     /locus_tag="ACHKUI_000928"
     CDS             47502..48491
                     /gene="sbp"
                     /locus_tag="ACHKUI_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6597184.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKDTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            48598..49353
                     /gene="cdh"
                     /locus_tag="ACHKUI_000929"
     CDS             48598..49353
                     /gene="cdh"
                     /locus_tag="ACHKUI_000929"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6597185.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(49408..50181)
                     /gene="tpiA"
                     /locus_tag="ACHKUI_000930"
     CDS             complement(49408..50181)
                     /gene="tpiA"
                     /locus_tag="ACHKUI_000930"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6597186.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAVAIAP
                     PEMYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRT
                     YHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAA
                     FEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNAS
                     NAAELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(50289..50888)
                     /gene="yiiQ"
                     /locus_tag="ACHKUI_000931"
     CDS             complement(50289..50888)
                     /gene="yiiQ"
                     /locus_tag="ACHKUI_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFO6597187.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            50989..51429
                     /gene="yiiR"
                     /locus_tag="ACHKUI_000932"
     CDS             50989..51429
                     /gene="yiiR"
                     /locus_tag="ACHKUI_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6597188.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            51641..51940
                     /gene="yiiS"
                     /locus_tag="ACHKUI_000933"
     CDS             51641..51940
                     /gene="yiiS"
                     /locus_tag="ACHKUI_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6597189.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            51967..52395
                     /gene="uspD"
                     /locus_tag="ACHKUI_000934"
     CDS             51967..52395
                     /gene="uspD"
                     /locus_tag="ACHKUI_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6597190.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(52400..53146)
                     /gene="fpr"
                     /locus_tag="ACHKUI_000935"
     CDS             complement(52400..53146)
                     /gene="fpr"
                     /locus_tag="ACHKUI_000935"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6597191.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(53243..54253)
                     /gene="glpX"
                     /locus_tag="ACHKUI_000936"
     CDS             complement(53243..54253)
                     /gene="glpX"
                     /locus_tag="ACHKUI_000936"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6597192.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            complement(54424..55932)
                     /gene="glpK"
                     /locus_tag="ACHKUI_000937"
     CDS             complement(54424..55932)
                     /gene="glpK"
                     /locus_tag="ACHKUI_000937"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6597193.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            complement(55955..56800)
                     /gene="glpF"
                     /locus_tag="ACHKUI_000938"
     CDS             complement(55955..56800)
                     /gene="glpF"
                     /locus_tag="ACHKUI_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6597194.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            57225..57470
                     /gene="zapB"
                     /locus_tag="ACHKUI_000939"
     CDS             57225..57470
                     /gene="zapB"
                     /locus_tag="ACHKUI_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6597195.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(57555..58040)
                     /gene="rraA"
                     /locus_tag="ACHKUI_000940"
     CDS             complement(57555..58040)
                     /gene="rraA"
                     /locus_tag="ACHKUI_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6597196.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(58133..59059)
                     /gene="menA"
                     /locus_tag="ACHKUI_000941"
     CDS             complement(58133..59059)
                     /gene="menA"
                     /locus_tag="ACHKUI_000941"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6597197.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(59126..60457)
                     /gene="hslU"
                     /locus_tag="ACHKUI_000942"
     CDS             complement(59126..60457)
                     /gene="hslU"
                     /locus_tag="ACHKUI_000942"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6597198.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(60467..60997)
                     /gene="hslV"
                     /locus_tag="ACHKUI_000943"
     CDS             complement(60467..60997)
                     /gene="hslV"
                     /locus_tag="ACHKUI_000943"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6597199.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(61090..62049)
                     /gene="ftsN"
                     /locus_tag="ACHKUI_000944"
     CDS             complement(61090..62049)
                     /gene="ftsN"
                     /locus_tag="ACHKUI_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6597200.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLVQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPAYTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(62141..63166)
                     /gene="cytR"
                     /locus_tag="ACHKUI_000945"
     CDS             complement(62141..63166)
                     /gene="cytR"
                     /locus_tag="ACHKUI_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6597201.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(63322..65520)
                     /gene="priA"
                     /locus_tag="ACHKUI_000946"
     CDS             complement(63322..65520)
                     /gene="priA"
                     /locus_tag="ACHKUI_000946"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6597202.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSIWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTNHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            65723..65935
                     /gene="rpmE"
                     /locus_tag="ACHKUI_000947"
     CDS             65723..65935
                     /gene="rpmE"
                     /locus_tag="ACHKUI_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6597203.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(65996..66604)
                     /gene="yiiX"
                     /locus_tag="ACHKUI_000948"
     CDS             complement(65996..66604)
                     /gene="yiiX"
                     /locus_tag="ACHKUI_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6597204.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAAGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKIKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(66664..66981)
                     /gene="metJ"
                     /locus_tag="ACHKUI_000949"
     CDS             complement(66664..66981)
                     /gene="metJ"
                     /locus_tag="ACHKUI_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6597205.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            67258..68418
                     /gene="metB"
                     /locus_tag="ACHKUI_000950"
     CDS             67258..68418
                     /gene="metB"
                     /locus_tag="ACHKUI_000950"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6597206.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            68421..70853
                     /gene="metL"
                     /locus_tag="ACHKUI_000951"
     CDS             68421..70853
                     /gene="metL"
                     /locus_tag="ACHKUI_000951"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6597207.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(71071..71931)
                     /locus_tag="ACHKUI_000952"
     CDS             complement(71071..71931)
                     /locus_tag="ACHKUI_000952"
                     /inference="COORDINATES: protein motif:HMM:NF011686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6597208.1"
                     /translation="MYIKKHWIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKS
                     HNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(72008..73627)
                     /locus_tag="ACHKUI_000953"
     CDS             complement(72008..73627)
                     /locus_tag="ACHKUI_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6597209.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSAPLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
     gene            complement(73668..75218)
                     /locus_tag="ACHKUI_000954"
     CDS             complement(73668..75218)
                     /locus_tag="ACHKUI_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6597210.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPFNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
BASE COUNT        18302 a        18717 c        19722 g        18567 t
ORIGIN      
        1 gtaggccgga taaggcactt gtgccgcatc cggcagtgtt tacccgcggc gactcaaaat
       61 ttctttctca taagtccgca cgttctccag ccattcgcta cccgctggcg tatcgtgacg
      121 ctgacaatac atttcccata ccgcctgcca cggcaacgat ttctgctctt ccagtagcgc
      181 cagacgcgca gtgtaatcgc ccgccgcttc cagcttgcgt agttcagcgg taggttccag
      241 caacgcacgc agcagggctt ttttcatatt gcgtgtacca atgacccacg cggcaatgcg
      301 gttgatagag gcatcgaaga agtcgaggcc gatatgcacc cggtcgaaca gatcgtgacg
      361 aacaatctca ctggcaatcg cctgggtttc atcatccagc agcactacgt gatcgctgtc
      421 ccagcgaacc ggacggctga cgtgcagcag caactgcggc acatacagca tggcggcgga
      481 aatcttgtcg gaaatcactt cagtcgggtg gaaatgcccg gcgtccaggc acagcgccgt
      541 ctggcggctg gtggcatacc ccaggtaaaa ctcattggag ccaaccgtgt agctctccgc
      601 gccaatgcca aacaatttgc tctcaacggc gtcgatatgg tgcgcagggt ttagcttctc
      661 gctgatcact tcatccagtg ctgccagcaa acgctgacgc ggggcgaggc ggtcaacggt
      721 gatatctttc atgccatccg ggatccagat gttcatcacc gacggtgtgc cgagttgctc
      781 accaaaatag gctgaaacac ggcggctggc tttacagtgg tcaatccaga actggcgaat
      841 gcggtcgtcg gcatgggaaa gcgtaaagcc atcggcgctt agcggatgtg aaaaacagga
      901 ggggttaaaa tccagaccga gctgattggc tttcgcccat tcaacccagt ttttgaagtg
      961 ctctggtttg atctggtcgc gcgagactgg cgtgtctgat tccagataga tggcatgtaa
     1021 attaagccgt ttcggccctg gaatcagccg catagcctgt tccagatcgg cacgtagctc
     1081 actggcatta cgcgctttgc ccggataatt accagtagcc tgaataccac cggtcagcaa
     1141 accttccggg ttttcaaaac cggaaacatc atcgccctgc cagcagtgca ttgaaacggg
     1201 taaacgatca agttggcgca gtgcctcctc gacatcaatc cctactgccg cgaaacgctg
     1261 tttcgcaagc tcccaggcct gttccagttg agtggtcatg cgcaaagctc ctttgtctgt
     1321 cgtgtagagt gaatctgcgc cacatagtgg gcaatttcac tgtcaggatt aggggtaaag
     1381 gtggtcagat tcgcggtagt gctgacgacc tgacggaaat catccacatt gttgagttca
     1441 tccagcgtca ttaactggat gccgatattg ccgagcgtcg aggcttcaac aggccctgcg
     1501 atcacccgaa taccgcaggc atcggcacac agctggttga gcagcgtgtt ctggcagccg
     1561 ccgccgacaa tatgcagttg cgagaaatct tcaccacgca gctgcgccag ctcatgcaac
     1621 acatcggcat acagcagcgc cagactgtcg aaaatacagc gcgccagttc agcatcactt
     1681 tccgggatcg gttgcgccgt ttcccgacac gcagcctgaa tttcgctgca catctcgtca
     1741 ggattaataa agcgatcgtc attgggattg atgatgaagc ggcaggccgg aagtgcctgt
     1801 gttgcggcga taagcgccgg aagatcgttg atttgccgct cctgaagcac tcgctgaagc
     1861 agccataagc ccataatatt tttcagcacc cgatagcgac cttccgcccc gccttcattg
     1921 gtgatattgg ctgccagcgc cgtgtcattg gtaaatggcg tctggctttc gaagcccatc
     1981 aatgaccagg tgccagaaga gagataagcg gcgcgtgaac cgtttaacgg cgaggcgata
     2041 accgcgctgg cggtatcatg gctggcaacg gcgaccaccg gaatctcatt accctgcggg
     2101 caaatccagt gacctatgac attacccgga tgcgtcgggc gaccaaacca ggctttgttg
     2161 gcaccgctcc acgccagtag cgactcgtcc cagtcgtcgc tattgatatt gaccagttgc
     2221 gtggtcgtgg cgttggtata ttcccagttc atcttgccgg tcaggcgata actaaagtaa
     2281 tccggtatca gcaaagcgtg agcaatgtgt ggaataagtt ctggttgctg ctctgtcagt
     2341 gcacgtagct gatagatcgt attgaagggc agaaactgga tgccgctacg ttgataaata
     2401 tcgcgtttgc cgagttgttg ttgtgcctgc gccattaggc cattggtgcg gctatcgcga
     2461 taagcaacgg gcaggcccac acgctgaccc tgttggtcga gcagcacaaa gtccacgccc
     2521 caggtatcaa tcccaatgct atcgatacga atcccttcct cgcacacctt gtttaatcca
     2581 aggcgaatgg cactttccag gctatccaca ttccaggtga cataaccgtt ctggctatgc
     2641 aggccattgt taaaacgatg gatttcgcgc agcgtcaggc tgcggcattc acgctcgtaa
     2701 cgcgccagca tcacgcgccc actggatgcg ccgagatcga cggcgacaca attgcgaaag
     2761 gtcataatgt gatcctgctg aatttcatta cgaccagtct aaaaagcgcc tgaattcgtg
     2821 accttctcgt tactgacagg aaaatgggcc attggcaacc agggaaagat gaacgtgatg
     2881 atgttcacaa tttgcttcat tgtggtgatg tgatgctcac cgcatttcct gaaaattcac
     2941 gctgtatctt gaaaaatcga cgttttttac gtggttttcc gtcgaaaatt taaggtaaga
     3001 acctgacctc gtgattacta tttcgccgtg ttgacgacat caggaggcca gtatgaccgt
     3061 attatatagt gtggattttt ttccgtctgg taacgcgtcc gtggcgatag aaccccggct
     3121 cccgcaggcg gattttcctg aacatcatca tgattttcat gaaattgtga ttgtcgaaca
     3181 tggcacgggt attcatgtat ttaatgggca gccctatacc atcaccggtg ggacggtctg
     3241 tttcgtacgc gatcatgatc ggcatctgta tgaacatacc gataatctgt gtctgaccaa
     3301 tgtgctgtat cgctcgccgg atcgatttca gtttctcgcc gggctgaatc agttgctgcc
     3361 acaagagcag gatgggcagt atccgtctca ctggcgcgtt aaccacagcg tattgcagca
     3421 ggtgcgacag ctggttgcac agatggaaca gcaggaaggg gaaaatgatt taccctcgac
     3481 cgccagtcgt gaaattctgt ttatgcaatt actgctcttg ctgcgtaaaa gcagtttgca
     3541 ggagaacctg gaaaacagcg catcacgtct caacttgctt ctggcctggc tggaggacca
     3601 ttttgccgat gaggtgaatt gggacgctgt ggcggatcaa ttttctcttt cattgcgtac
     3661 gctgcatcgc cagcttaagc agcaaacggg gctgacgcct ctgcgttacc tgaaccgcct
     3721 gcgactgatg aaagcccgac atctgctacg ccacagcgag gccagcgtta ctgacatcgc
     3781 ctatcgctgc ggattcagcg acagtaacca cttttcgacg ctttttcgcc gagagtttaa
     3841 ctggtcaccg cgtgatattc gtcagggacg ggatggtttt ctgcaataac gcgaatcttc
     3901 tcaacgtatt tgtacgccat attgcgaata atcaacttcg ttctctggcc gaggtagcca
     3961 cggtggcgca tcagttaaaa cttctcaaag atgatttttt tgccagcgac cagcaggcag
     4021 tcgctgtggc tgaccgttat ccgcaagatg tctttgccga acatacacat gatttttgtg
     4081 agctggtgat tgtctggcgc ggtaatggtc tgcatgtact caacgatcgc ccttatcgca
     4141 ttacccgtgg cgatctcttt tatattcatg ctgacgataa acactcctac gcttccgtta
     4201 acgatctggt tttgcagaat attatttatt gcccggagcg gctgaagctg aaccttgact
     4261 ggcagggagc gattccggga tttagcgcca gcgcagggca accacactgg cgcttaggta
     4321 gcgtggggat ggcgcaggcg cggcaggtta tcggtcagct tgagcatgaa agtagtcagc
     4381 atgtgtcgtt tgctaacgaa atggctgagt tgctgttcgg gcagttggtg atgttgctga
     4441 atcgccatcg ttacaccagt gattcgttgc cgccaacatc cagcgaaacg ttgctggata
     4501 agctgattac ccggctggcg gctagcctga aaagtccctt tgcgctggat aaattttgtg
     4561 atgaggcatc gtgcagtgag cgcgttttgc gtcagcaatt tcgtcagcag actggaatga
     4621 ccatcaatca atatctgcga caggtcagag tgtgtcatgc gcaatatctc ctccagcata
     4681 gccgcctgtt aatcagtgat atttcgaccg aatgtggctt tgaagatagt aactattttt
     4741 cggtggtgtt tacccgggaa accgggatga cgcccagcca gtggcgtcat ctcaattcgc
     4801 agaaagatta attcgccatg ccgatgccga cgatgttagc ggcgacaata atcaccacac
     4861 aaccgaggct caacaccgtt accggacggc gtcctgcatt gttccactct ttcagcacca
     4921 gcccgacgat accgccgcac aatacataga agctcatatg cagcatccaa ctgatgtagt
     4981 catactgcgc cggaatgcgg gcgtggcccc aggcatagaa aaagaattgc agataccaca
     5041 tcaacccgcc cagtgctgag agtaacacgt tgtgaatgat cagcggtttt gccagcgaga
     5101 agtcggcttt tagcgacaaa tccttcactt ttgccagacg aataaaacag aaaccgaggt
     5161 taatgatcgc gccgcctccc atgatgacaa catagcttgg cagagcgaca tacaggggat
     5221 cgacgcccag tgcggcagcg gcttcatgca tcggttttgc ggcgttcatc gcaaaggaca
     5281 tcccggcaga gaaaatgccg cacatcaccg ccagcaccag cccttttttc aggttgaact
     5341 cttcggcttt aatgcccatc ttgcgctctt tcaactgccc ggcgcgagtc acaatcccta
     5401 cgccaatcag cgccaccaga acgccgagca acgtcatgcg tccgccttcg gtattaatca
     5461 gaacatcgaa attgccgttg ataattggcg tcatcagcgt accgacaatc aacgtgatac
     5521 caatggcgat accaatcccc atcgacatgc cgagataacg catggtcagg ccgtagttga
     5581 tattaccgat cccccacata gcgccgaaca gaaaaacagg cagcagcgta gagagactaa
     5641 acgagctgta atacgcccag aaattcggca gtaacagggc gctgatggcc cacggcagga
     5701 taatccacga aacaatccca ccaactgacc acatggtttc ccatgaccat ttttttactt
     5761 ttttgaacgg agcgtaaaaa caggctgcac tggccgcgcc gatcaaatgc caaaatatcc
     5821 ccatcgtaat cgcgttactc atcttcttat cctcatcatt ttttcgtcgc gtcacatctc
     5881 cgacgagatg agtgtaaaaa ttgagctgtc gattaacctt tcgcctgttg ccgccgctgc
     5941 cgatttactg gcaatcacgg cattaagtgg gtgatttgct tcacatcttg ggcattttcc
     6001 tgcaaaacca tacccttatg aaaagtacgg cattgataat cattttcaat atcatttaat
     6061 taactataat gaaccaactg cttacgcggc attaacaatc ggccgcccga caacactgga
     6121 gatgaatatg agttataccc tgccatccct gccgtatgct tacgatgccc tggaaccgca
     6181 cttcgataag cagaccatgg aaatccacca caccaaacac catcagactt acgtcaacaa
     6241 cgccaacgcg gcgctggaaa acctgccaga atttgccaac ctgccggttg aagagctgat
     6301 caccaaactg gaccagctgc cagcagacaa gaaaaccgta ctgcgcaata acgctggtgg
     6361 tcacgctaac cacagtctgt tctggaaagg tctgaaaaaa ggcaccaccc tgcagggcga
     6421 cctgaaagcg gctatcgaac gtgatttcgg ttccgttgat aatttcaaag cagaatttga
     6481 aaaagcggca gcttcccgct ttggttccgg ctgggcatgg ctggtgcaaa aaggcgataa
     6541 actggcggtg gtttctactg ctaaccagga ctctccactg atgggcgaag ccatttctgg
     6601 cgcttccggc ttcccgattc tgggcctgga tgtgtgggaa catgcttact atctgaaatt
     6661 ccagaaccgc cgtccggact acattaaaga gttctggaac gtggtgaact gggacgaagc
     6721 agcggcacgt ttcacggcga aaaaataatc atttgacgcc tgctgcaatg aggcgtttac
     6781 gccgcattgc agcttaaaaa atgaaccatc gccaacggcg gtggtttttt tgtgatcaat
     6841 ttcaaaataa aaacaatgat ccgataaaaa taaaacagcg tttcaattga tgtggttttg
     6901 atcactttta ttgatcaaat gaatgtctat cttcgtttcc atcaacactg atgctccgtt
     6961 gaggaatgac gcatcagccc ttaaaaatat gccgacaggt gatggaaatg cagataaaac
     7021 gctcgattga gaaaatcccg gggggatgat gctcgtcccg ctattccttg gcgcactgtg
     7081 ccacaccttc tcgccggggg cggggaaata ttttggatca ttcaccaacg ggatgattac
     7141 cggtacggtg cccattctgg cggtgtggtt tttttgcatg ggggcgtcaa taaaattaag
     7201 cgcgacggga acggtactgc gtaaatccgg tacgctggtg gtaactaaaa ttgccgtcgc
     7261 gtgggtggtt gcggcaattg cctcgcgcat tattccggaa catggtgttg aagttggatt
     7321 ttttgccgga ctttcaacgt tggcgctggt ggcggcgatg gatatgacca acggcggact
     7381 ttacgcttcc atcatgcagc agtacggcac aaaagaagaa gctggggcat ttgtgttgat
     7441 gtcgttggag tccgggccgc tcatgacgat gattattctg ggcactgccg ggattgcctc
     7501 gtttgaaccg catgttttcg tcggcgcagt attgccgttc ctggtgggct ttgccctggg
     7561 gaaccttgac cctgagttac gagaattttt cagcaaagcg gtgcagacgc tgattccatt
     7621 ctttgccttc gcactgggca ataccattga tttgactgta attgcccaga caggtttgct
     7681 ggggatcctg ttgggtgtgg cagtaattat cgtgaccggt attccgttga ttatcgccga
     7741 taaattgatt ggcggtggcg atggcactgc cggaattgct gcttccagtt ccgcaggggc
     7801 cgcggtagcg acacctgtgc tgattgctga aatggtgcct gcgtttaaac cgatggctcc
     7861 ggcagcaact tcgctggtcg cgacggcggt cattgtgact tcgattctgg tgccaattct
     7921 tacctctatc tggtcacgta aagtcaaagc cagagtggcg aaaatcgaaa ttttaggcac
     7981 ggtaaaataa acctgcttcc ttaatcccca cagccggatg aactaacgtt cgtccggttt
     8041 tcaattgcgt ttctgcctgg atacttaccg cgaaaacagc acgcctgaca taaaataaat
     8101 atggtctaat gggaaaattt ctcgtggaga gggaacagat gcgatatccg gttgatgtat
     8161 acacaggcaa gattcaggct tatcccgaag gcaaacccag cgcaattgcc aaaatccagg
     8221 ttgatggtga gttgatgctg acggacctgg ggctggaagg tgacgaacag gcggagaaaa
     8281 agatccacgg cgggccagac agagcgctgt gtcattatcc tcgtgagcat tatctctact
     8341 gggcgcggga atttccggaa caggcggagc tgtttgttgc gcctgcgttt ggcgaaaatc
     8401 tctcaaccga cggtctgacg gaaagtaatg tttatattgg tgatattttc cgctggggag
     8461 aggcattaat tcaggtcagc cagccacgct caccttgcta taaactcaat taccattttg
     8521 atatcagcga tatagcgcag ttgatgcaaa acacgggtaa agtggggtgg ttgtatagcg
     8581 taattgcacc aggggtggta tctgctgatg cgccgctgga gttggtttcc cgtgtcagcg
     8641 atatgaccgt gcaggaggct gccgccatcg catggcatat gccgtttgat gacgatcagt
     8701 atcaccgttt actctccgcc gcagggttat cgaaaagctg gacgcgaacg atgcaaaagc
     8761 gccgacttag cggcaagatc gaagattttt cccgccgttt gtggggaaaa taaccccgga
     8821 gtgtaggcct gataagacgc tatcagcgtc gcatcaggca tcctgctcaa atgccggatg
     8881 cggcgtaaac gccttatcct gcctacaaaa cccataaatt caacaggttt tgtaggcctg
     8941 ataagcgtag tgcatcaggc atttttttta cctgaatgtt gacgttatga ccgcttatac
     9001 agcggcaacc aaatcaccag ccgtaaaccg cccagcgggc tgtcttctgc cttcacccag
     9061 ccacgatgct gctgaatggc ggtttctaca atcgccagcc ccaaacctgt accgccagat
     9121 tcacgatcgc gtgcttcatc ggtccgatag aacggacgga aaatctgttc gcgatcttcc
     9181 gggctaacgc caggaccatc gtcgtccacc gtaatggtga taccgtcttt atctaccgca
     9241 aagcccactt caatcttcgt atgggaataa cgcagagcat tacgaacaat gttttccagc
     9301 gcactttcca gggcgttcgg gttgccgtac agcggccacg gcccaggtgg gaagttaacc
     9361 gtcaacgact tgcccatttg ctcggcttcg aacgccgcgt tatccagcac ttcactccac
     9421 aactggttgg ctttgatggt ctcgctaacc agcgcatttt tctgctgatt acgtgacatc
     9481 accaacagat cgttgatcat gctgtccaga cgttgcgctt cggtttcaat acgctccagt
     9541 tccttgcttt caccgctacg acggcgcagt aacgccgtac ccagttgcag acgcgtcagc
     9601 ggggtgcgca gttcgtgaga gatatcagaa agtagacgct gctgagaggt catcatgcgc
     9661 tccagcgcgg tgaccatctg gttaaaactg gcaccggcgg caaggaattc ctgtggcccc
     9721 gcttccagtt ccgggtgctg gcgtaagttt ccctgggcaa cttcatcggc agcgtttttc
     9781 agcttacgcg ccggttttgc cagactccag gccaaccaca acagcagcgg cgtactgacc
     9841 agcatggtga caatcagcag taatagcggg cggtcaaaca gtaagttaat gaaatcggat
     9901 tgagaactgc tggccggacg aatcagataa agttggtaat tatcttcgcc atcacgcacg
     9961 gagaacggac cgaccagttc cacgcggcca tactttttct tctgcggatg atcggcgtta
    10021 tcggcctgac caataaagtt acgaatgatc tgcatttcgc tgcgttcagc gccgatcacg
    10081 cggccttcgg tggtcaccaa taacaaacgc tgtcctggcg gtgcccactt atcaatcgcc
    10141 cggaacagac gccgccacca cattaaatcg ttgggcggat cgttcgccag ctccgcttcg
    10201 acatgctgct caatcatcag accctgacgc tgttcgctat ccagaagctc ggtcatctgg
    10261 cgtgaatcga gcttgggtaa catcaaaacc aacatcaaca ccagcgccag cgtcagccag
    10321 aagatggcga agatgcgcgc ggttaagctg cctatcatga agcagaaacc atcagatagc
    10381 cgcgaccacg cagggtttta aaccacgggt gaccatcttt acgatccggc agtttacgac
    10441 gcaggttgga aatgtgcata tcgatagcgc ggtcgaaagg cgtcaggcgc ttgcccagca
    10501 cttcctggct taaatgctca cgggaaacca cctgacccag atgctgtgcc agcaaataga
    10561 gcagggtaaa ctcagtaccg gttaactcca gtgtttgccc gtcgaagctg gcttcctggc
    10621 gaccaggatt gagacttaag gcatcaactt ccagtgtcgg tgaaccgttg tcgttgtttt
    10681 gctgttgttc gctccagtgc gaacggcgca ggatcgcgcg aatacgtgcc accagctcac
    10741 gatcattaaa cggttttggg agatagtcat ctgcgcccag ctcaagcccg agaacgcgat
    10801 caagttcact gccgcgcgcc gtcaacataa tgacaggcgt ctggtgtgtc tggcgaagtg
    10861 cttttaatgt gtcgatacca tttttcttcg gcatcattac atcaagcaaa agtaaatcaa
    10921 tgctgtcgtc cagaagatca agcgcctgtt ccccatcgtg ggcaacaatc acgttgaagc
    10981 cttccatctc gagcagctcc tttaataggg aagtcagctc tcggtcatca tcaactaaca
    11041 ggattttatt cattgtttaa atacctccga ggcagaaatt acgtcatcag acgtcgctaa
    11101 tccatgactt tacgttgttt tacaccccct gacgcatgtt tgcagcctga atcgtaaact
    11161 ctctatcgtt gaatcgcgac agaaagattt tgggagcaaa tgatgcgcat agttaccgct
    11221 gccgtcatgg cctcaacgct ggcagtcagt tcattaagcc acgctgctga agtcggttca
    11281 ggcgataact ggcatccggg tgaagaactt acgcagcgca gtacgcagag ccatatgttc
    11341 gacggcataa gtttaaccga acatcagcgt cagcagatgc gagatcttat gcaacaggcc
    11401 cggcacgaac agcctcctgt taatgttagc gaactggaga caatgcatcg ccttgtcacc
    11461 gcagaaaatt ttgatgaaaa cgctgtgcgc gcacaggcag aaaaaatggc gaatgagcaa
    11521 atagctcgtc aggttgagat ggcaaaagtc cgcaaccaaa tgtatcgcct gttaacgccg
    11581 gagcagcaag cggttttaaa cgagaaacat caacaacgaa tggagcagtt gcgcgacgtg
    11641 acgcaatggc aaaaaagttc atcgttgaag ctattgagta gtagcaactc acgttcccag
    11701 tagtaaaccc tgttttcctt gccatagaca ccatccctgt cttcccccac atgatgtggg
    11761 ggtttttttt atcaattact tacatgcaat caaaagtaaa aacagtcact tatcgaaagc
    11821 tgtaagggtt tggagtggtt tcaaaatttc ttgagtgtgg acactatgtg gacactcaga
    11881 gggggaaatt tataacgtca ctccgccttt tagtggattc agagcgacgg cattttgcag
    11941 gtagtcaggc gcaaggtgcg cataggccat cgtctgctga atgctcgcat gtcccagaat
    12001 ctgttgcagt gcgattatat tgcccccgtt catcatgaaa tggcttgcga atgtatgccg
    12061 caggatgtgg gttgcctgat tgggtggtat atcaggtttc actctgcgta aaatcccgca
    12121 aaatttctca taatcaactt tgaataattt ggcgcttgcc tcctctttaa cttttttctc
    12181 cagttcctca gaaatcggca cggttcgctt tttaccgttt ttggttttca ggaaggtaac
    12241 cctgcaattt gtaatctgtg ctggttttag cgtggcaact tccgtccatc ttcctccagt
    12301 gctcagacat aaaagcgcga caagtaagtc atcaccagcc aaaacattta acagtttttc
    12361 gatttctgct ttttccagga acgtcatttc agggttggcc tccgccagtg gcggcagtcc
    12421 gtgaattggg tgttgcccgg aaaattcatc caattgaatt aattttgtga acatgccgga
    12481 taatcggtac atgtcacggt ttatcgttgc ggcactgata ccatcacgta gtcgtatgga
    12541 acgataatcc atcaaagccc ttttgctcat ccggctcact ggtatatcac ctatgccgct
    12601 gatggttttg agtagatgat taaactcttt tgttccatgc tcgtggtttt gcccgtgata
    12661 tttccaccag atgtccagca actcactcaa agttcggcgg tctgctcgct ggcctcccca
    12721 ttctttctga ctggcattgg cgattgtgta tcgctcaaat gctagtgctt cagcttttct
    12781 ttcgaatttc ctgcggatgc gttttccgtc gcgaccgcga ggtctaatgt ccacttcata
    12841 gcgaccatca tcgagcttct taattgccat aagaaagccc tccggcgctg tattcaccat
    12901 cttggtagca aatggtgaaa atgtaatctt tatatagagt tagccaatcc ttttcgcgga
    12961 gtggttggac tctgttgact ctggcccaat gtgcgcgaga gccggtgcga tttgtcctgc
    13021 gtccggcgcg gttttatctg tcataagcca tagagcatat ttttggaatg tgggatgcat
    13081 agtgattttt agcaaagctg tgccaccggg ttcaaagttt cctccttcat attttttaag
    13141 tgtgcttagc ggtaactcta tgatttcaca gaattttgat tggcttagcc cttcagcctc
    13201 acgcaaggcc ttaatctttt cgcttaattt catttgacat ggtgcctata tagggactaa
    13261 attccctcaa aactggaacc tatataggtt ccattgattt gagaataaac cagcgtctaa
    13321 acggttttga gtggtttaga aagggctgga tcctatgagg gtaccatata tggacgctga
    13381 aaattatgtg attcagtatc cgcttgatgc ggttcatgtg gataaatttg ctgatttatt
    13441 agggaagcca aagacagccg tcagtgaaat ggtgaaggca aataaattac caattattga
    13501 attgcgtgat ccttgcaaac tgaaggctcg tgccggtgaa aaatgggttt tcattcctga
    13561 gtttaatcgc gctgtacgtg aggcgtttta taaccgaccg gttgaacagc gtgatgcatg
    13621 gcttttgtgg atggggttgt gattatgaat gagccgcgtt gtattgctca gttactgcgt
    13681 aacgaaagcc ccagggcgat tgacttcacc atcacccacg ggaaggggcg caagggaatc
    13741 attatccgca ccaaaaaaca gagtccgtta aagaaggctc tgacctttct gaaaagccgg
    13801 agggtatgga aatgacagtg atgacgctca atctcgttga aaaacagcca gcagctatgc
    13861 gccggataat tggtaagcat cttgccgtcc ctcgctggca ggatacatgt gattattata
    13921 atcagatgat ggagcgcgaa cggttaacgg tttgctttca tgcgcagtta aaacagcgtc
    13981 acgcaacgat gcgttttgaa gaaatgaacg acgtcgaacg tgaacggctg gtttgtgcaa
    14041 ttgatgaatt gcgtggggca ttctcaaaac gccgtcaggt tggcgcaagt gagtatgcat
    14101 atattagttt tttaacagtc agtcagcgtc gtactttatt tatgcatgcc ggattgactg
    14161 aaaaagaatt caaccagcca tactggcgaa ttaatgaaga atcatgttac tggcgtgatg
    14221 ctttattccg tgcattacgt gaattattca gcctgtttga gtatgcaccg acaattctga
    14281 cgtcggtaaa accagagcaa tatctgcatt aaataattaa ccagagtttt taacgcactt
    14341 aatcgtgcgg ggcttctttt tgcctggaga aagtcatgca tacagtttct gaaaatcagt
    14401 gcggtatata cgcattactg ctgcaacagg ccagaaccga agcacaggcc gacgctgcga
    14461 cgcgcttttc ttctcatctt gacgccatga ttcgccacat cacaaaggcg gagttatccc
    14521 gcgtggagat agtcgagctg ctcagtcagg tgtcggaaaa atttcacaat atcggattgt
    14581 ctcgcgggga ggtactttga tgtcctgttc tcattcagtt gtattactga ataacgcctt
    14641 aaaaatcgcc gttatggaaa atggtgattt gtctcttatt caactttgtc ttgataaaga
    14701 aaaacgcgac ataactgaat ctgttatcgc gatttatcag aatgaattaa acctcctgtc
    14761 tgatgtggtc aatttacttg ttaaacgcgc tgtgttccac aagcaaattt cctcagtgga
    14821 tgaactgaca aaattaacga cagaactcgc cagttattgc gctgatgtat ccaggaaact
    14881 taacgataaa aggagctgat aatgccggac aacgtagatt ttattcagga acaacaggct
    14941 gaattactgg agcgccagat taacgcggca agggtaaaac attgctgtgc ttctgcgctg
    15001 gtttgcgaag agtgtgatac gccaatacct gctgcccgtc gtgcggctta tccgtcagcc
    15061 acgcgttgtg tttcctgcca gtcagtcttt gaagcaaaaa acaagcatta ccggagaatg
    15121 gcatgagtat tcgtattgaa attggcgaac gttatgtcgt taccagtgac agctttcagt
    15181 ttattctcca cgagaaaaag agagctgaaa gcggtaaaaa cgccggtcag gaatggctgg
    15241 cggtggttgg ttattacccg aaattaagcc agctcgtttc cggcctgatg catcacgata
    15301 ttctgaccgg aagcgcaaag tcttttgctg atttaaacgc gcaggttgag caactcagca
    15361 ggcgttgttc agaggctttt ggctcatatg gccgttaaag cctccgggcg ttttgtccct
    15421 ccgtcagcat ttgccgcagg caccggtaag acgtttaccg gtgcttatgc atggaacgcg
    15481 ccacgcgagg ccgtcgggcg cgaaagaccc cttacacgtg acgagatgcg tcaggtgcaa
    15541 ggtgttttat ccacgattaa ccgcctgcct tactttttgc gctcgctgtt tacttcacgc
    15601 tatgactaca tccggcgcaa taaaagcccg gtgcacgggt tttatttcct cacatccact
    15661 tttcagcgtc gtttatggcc gcgcattgag cgcgttaatc agcgccatga aatgaacacc
    15721 gacgcgtcgt tgctgtttct ggcagagcgt gaccactatg cgcgcctgcc tggaatgaat
    15781 gacaaggaac tgaaaaagtt tgccacccgt atctcatcgc agcttttcat aatgtatgag
    15841 gaactctgcg atgcctgggt ggatgcgcat ggcgagaaag aatcactgtt tacggatgag
    15901 gctcaggatc acctgtatgg tcatgttgct ggcgctgcac gagctttcaa tatttcccct
    15961 ctctactgga aaaaataccg taaaggacag atgaccacga ggcaggctta ttctgccatt
    16021 gcccgtctgt ttaacgatga gtggtggact catcagctta aaggccagcg tatgcgctgg
    16081 catgaggcgt tactgattgc tgtcggggag gtcaataaag accgttctcc ttatgccagt
    16141 aaacatgcca ttcgtgatgt gcgtgcgcgc cgccaggcaa atctggagtt tcttaaatca
    16201 tgtgaccttg aaaacaggga aaccggcgag cgcatcgacc ttatcagtaa ggtgatgggc
    16261 agtatttcta atcctgaaat tcgccggatg gagctgatga acaccattgc cggtattgag
    16321 cgttacgccg ccgcagaggg tgatgtgggg atgtttatca cgctgaccac gccgtcaaag
    16381 tatcacccga cacgtcaggt cggaaaagga gaaagtaaaa ccgtccagct taatcacggc
    16441 tggaacgatg aggcatttaa tccgaaggat gcgcagcgtt atctctgccg tatctggagc
    16501 ctgatgcgca cggcattcaa ggataatgat ttacaggtct acggtttgcg tgtcgtcgag
    16561 ccacaccacg acggaacgcc gcactggcat atgatgcttt tttgtaatcc acgccagcgt
    16621 aaccagatta ttgaaatcat gcgtcgctac gcgctcaaag aggatggaga cgaaagagga
    16681 gccgcgcgaa accgttttca ggcaaaacat cttaaccggg gcggtgctgc ggggtatatc
    16741 gcgaaataca tctcaaaaaa catcgacggc tatgcactgg atggtcagct cgataacgat
    16801 accggcaggc cgctgaaaga tactgctgcg gctgttaccg catgggcgtc aacgtggcgc
    16861 atcccgcaat ttaaaacggt tggcctgccg acaatggggg cttaccgtga actacgcaaa
    16921 ttgcctcgtg gcgtcagcat tgctgatgag tttgacgaac gcgttgaggc tgcacgtgcc
    16981 gccgcagaca gtggtgattt tgcgttgtat atcagcgcgc agggtggggc aaatgttccg
    17041 cgtgattgcc agactgtcag agtcgcccgt agcccgtcgg atgacgttaa cgagtacgag
    17101 gaagaagtcg agagagtggt cggcatttac gcgccgcatc tcggcgcgcg tcatattcat
    17161 atcaccagaa cgacggactg gcgcattgtg ccgaaagttc cggtcgttga gcctttgact
    17221 ttaaaaagcg gcatcgccgc gcctcggagt cctgtcaata actgtggaaa gctcaccggt
    17281 ggtgatactt cgttaccggc tcccacacct tctgagcacg ccgcagcagt gcttaatctg
    17341 gttgatgacg gtgttatcga atggaatgac ccggaggtcg tgagggcgct caggggcgca
    17401 ttaaaacacg gtcggagaac gccaaaccgt cagcaaagaa acggaagccc gttaaaacca
    17461 tatgaaatgg caccatcggc cagactgacc cggtcggaac gaatgcaaat tacccgtatc
    17521 cgtgttgacc ttgctcaaaa gggtatcagg ccgcagcgat gggaacttga ggcgctggcg
    17581 cgtggagcaa ccgtaaatta tgacgggaaa aaattcacgt atccggttgc tgatgagtgg
    17641 ccgggattct caacagtaat ggagtggaaa tgatggcaaa aattcacgag gtaaagctgc
    17701 acgcaaaata tttcgacctt gtgctggaag gaaagaaacg tgcagagttt cggaaaaatg
    17761 accgtaatta tgagcgcggg gacacgctga ttttgcatga atgggtacag ggtgtgttta
    17821 cggggcgaaa ggttgaagcc cggataacag atgttactga cctgtcagac tggctggaag
    17881 attatgtctt gctaagtatt gagctgctta atacaggcgc atatgagatt gtgaactgga
    17941 aagaacttag tgagcgtggt ctggtattca gaattaatca tgaaattatg catcagctcg
    18001 gtcttgctgt tatgtatgag tcagtgacag gcctgtctgg cggagcagtg gttgctgcgg
    18061 atgggatgtg gagctattca gatgaacagg tggagtgtgc aaagcaaaaa gggtggtttt
    18121 gaaaatgcac agaataccag gcgagatacc tcagcataaa actaaaaata tcaagctgat
    18181 ggccattgtt caccgtctac agcagattat ggtcaacgaa aatctgacgc ctgatgagct
    18241 ggtcgggtgt gccgaaatgg tccgggataa ttacagacgg tttaacgata tcagtagaca
    18301 gaaaattgag gtatttcgac cagcaaacat gcctaagaaa ctaccgcata ggatgtagta
    18361 tactcgccat ttctgaaact tgttttcaga gatggcgggg ttgaacaatg agcgtagcga
    18421 cgtgttagct ttgattcctg ctgtgtttac aattaaacct attgaaaatt aatgagaact
    18481 caatttcatc tttctctcgt taaagatggc cacaactact aaaacatgag aatatcaaaa
    18541 cttggcgaaa tctagctttt cccaaggggt ggtttcgcta taatacgcgg tcggttttta
    18601 ggaggggcga tgccaactgt agtttcactt ttttctggat gtggtggttc tgatgcggga
    18661 gttttgagag cagggttcaa tgtgctgatg gcaaacgata ttttgcctta tgcaagggac
    18721 gtttacttag aaaaccatcc tgaaactgat tacatattgg gcgatatttc aggactccaa
    18781 tcgttccctt ctgccgaatt gcttatcgga tgctatcctt gccagggatt tagccagggt
    18841 ggagcaagga aggcagatag aaagattaat actctatatt tagagtttgc tcgtgctttg
    18901 agaaagatta agccaaaagc attcattgta gagaatgttt ctggaatggt aaggcgtaac
    18961 tttgaacatt tgttaaagga tcaattcaaa gttttcgaag aagcaggtta tacagtaagc
    19021 tcgcaaattc tgaatgcgtc ccactatggc gtatcacaag ataggaagag aatctttatc
    19081 gtaggaatac gaaaagacta tggtattaca tacaaatttc caaaaccaac acatggtgat
    19141 ggcttgaaac catattcaac aattcgtgat gctattggtc atatgcctgt ttggcctatt
    19201 ggtgagtttt atgatgccga ttttcattgg tattatctat cgcgcaaccg tcggcaagat
    19261 tgggatcaga tatctaaaac aattgttgca aatcctagac atatgcctct acatccaata
    19321 agtccaacat tagaaaaatt gggtccagat aagtggcaat ttacttcgga tgcaccagct
    19381 cgtaggttta gttatcgaga ggccgctatt ttacagggtt ttggagacct aatttttcca
    19441 gaaactgaac gggcttctat taatatgaag tatacagtag ttggcaatgc tgttccgcct
    19501 cctttatttg aggcggttgc taaatctctt ccagatattt gggattagtt attcgacctt
    19561 aagatcattg ataatttgtt cgaaatcaag aaaccattgt tctgaaacta ttttagggca
    19621 atggtttgtc ttttttaaaa gctgtaatat ccttgctctg tcaatgatca atactccagc
    19681 gcaagggcta ttgtctaccc attgaccatc tgagtttcga tagaatacgg gggtaaacat
    19741 tagagttggt atgtcaaaat gcacttgaaa atatgttcgt agtttcaatg agtgggcttc
    19801 aagtgttttt ttgggccaat ctctttcttg ggctccacat tgacctaaaa aggcaaagtt
    19861 actacttaat ttatcttcaa attctatagt tgcaactata tcaaaaccac catcacctct
    19921 cggatcagct ctttcgcact cttgctcatt aatataagga acagctaagt cttttcctag
    19981 ccttttcaag gcttttctta aatcagtacc ataatatttt cgcctgtcat ctgagtttgc
    20041 gtcaaatact cgtacagttg agtgtgctgg gagtagtgct gttgcacaaa atttacttac
    20101 gacagcaaaa ttttttgccc aaaactgtat tattccttgc ctagcctttg tgaaagaacg
    20161 taacctcgag catgcgagta agaaaatata tattctttgt atctcagtga ggttttcttt
    20221 caatgaaata aagtcaccat taataataaa tggataaata tccttgagaa aattttgtct
    20281 aaaatcgagt tgcgtccaga catcttcaac ttgtctttct aaacggtcat tgcgttcagc
    20341 atcgcagcct tcattttcat cttcttgata ttcttgatca atatcatcca cacttgtgtt
    20401 actttgagtt cttattgtta tgaggtcatt tttatgtaga tcataacgac cagaataatt
    20461 tataaccgta gcaagttctg caaggtctgc aaaaagatga ggaacactag gatgaatgtc
    20521 tccgatttta aaaatcttca tcgtcagaaa cctttttggc tttaattgta ttaccaataa
    20581 gtttgacatt ttgacttagg cgtcttgcaa cttccaatgc ttcaggattg taatcaatgg
    20641 ttgcaacaat tccagccgct tcaattaatg cagattcagc tttataaagt aacgtcatga
    20701 aatcttgttt taagtcttca gttaactgat acgcaacctt aaggtcagcc ccgtttctaa
    20761 aggacgttaa agccttgggg ttatcaataa cagaagataa taggcgtaaa tttcgggact
    20821 ctccaacctt tgttttcccg gaatcatcct ttttataaag ccatactgta agttctctgg
    20881 tattttctct atttatatga tgtgggtgaa ttattacatc attggatgta atatcaccat
    20941 attcgtcttt ttctgaaacg ccaacaaaca atccaatttt ctcgtcagct aaggctgtag
    21001 ataaaattga aaacttgatc gattcttcat ctaatccatc aatatcataa aagttattgc
    21061 tctccattac tttataaacg gctaaagcat ctagatttct cttgatatga tcttttctgc
    21121 taccgattgc acgagcaacc tgatgatatc gatcattagt cggtgagttt ggggaggtga
    21181 gaccaaaaag ttgctcgata taacgagcct tcgatagagg ttcccattgt ttgacaccag
    21241 tgatatgtct gaaacccaaa taaggtaaaa tttcagctct ggtatctcta acgatcactg
    21301 gtaatttttc tagtgtacct agtttatctt tagcgctttc ggcgatttct atcatccggg
    21361 agctgggtct gtcgcactca tatgggttat gaataagttt aactgccgtt aagcgacggt
    21421 tgccttccac aacggtgtac ttatctccct ctttaaccgc aataagaggc tcgccaggaa
    21481 aaaatccatt ttctgcgatt gcattcatca agtcttcaat agaggttgtt agagctatat
    21541 gatttagcat tgcttctggg gtacgttcaa caccttcagg aagacgtgga ttttttgtat
    21601 caagctcaag tttattgagg tcgatgtaat caaggctttc tgtcataact ctaatctcat
    21661 tagatatggt gatatgcaaa gattaacact ggagttaaac gtaggctaga actgcaatta
    21721 tgtgcattaa aatgcatgcg taagtaactc gctaaaatga agccataaac tcacagtggt
    21781 gctatgaata atatgttatg caactgcatt aaaaccgacc catgaagcgg gcgggcgagg
    21841 cggggaaagc actgcgcgct ggcggtggtg cggattttat tttttcagcg tctgagcgtg
    21901 tcgtgacggc gtttagattg tgcgccgggg cattggtgtg tctgcggggt gttttgtgcg
    21961 gtggtgagcg tgtgagggcg tgatgacggg atataaaaaa gccgcccgca ggcggcgatg
    22021 ttcagccgtt gtcagtgtcc agtgagtagt ttttaaagcg gatgacctcc tgaccgatcc
    22081 agccgtttat ctcgcggatc ctatcctgta acgggataag ctcattgcgg acaaagacct
    22141 ttgccacttt ctcaatatcg cccagtgacc cgacgttctc cggcttgccg cccatcaact
    22201 gaaaggggat gcggtgcgcg tccagcaggt cagcggcgct ggcttttttg atattaaaaa
    22261 aatcgtcctt cgttgccact tcactgagcg ggataatttt gatgccgtcg gctttcccct
    22321 gtggggcata gagaaacaga tttttaaagt tgttgcggcc tttcgacttc accatgtttt
    22381 cgcggagcat ttcgatatcg ttgcgatcct gcacggcatc agtgacgtac atgatatatc
    22441 cggcatgagc gccgttttcg taatacttgc ggcggaacag cgtggccgac tcattcagcc
    22501 aggcagagtt aagggcgctg agatattccg gcaggccgta cagctcctga ttaatatccg
    22561 gctccagcag atgaaacacg gagccgggcg cgaaggctgt cggctcgttg aaggacggca
    22621 cccaccagta aacatcctct tccacgccac gacgggtata tttcgccggt gaggtttcca
    22681 gtctgatgac cttaccggtg gtgctgtagc gcttttccag aaacgcatta ccgaacacca
    22741 gaaaatccag cacaaagcgg ctgaaatcct gctgggaaag ccacgggtgc gggataaacg
    22801 ttgaagccag aatattacgt ttgacgtaaa tcggtgagct gtgatgcacg gcagcacgca
    22861 gactttttgc cagaccggta aagctgaccg gcggctcata ccatctgccg ttactgatgc
    22921 attcgacgta atccagaatg tcacggcggt cgagtaccgg caccggctcg ccaaaggtga
    22981 atgcctccat tttcggggcg ctggcggtca ttgtttttgc cgcaggttgc ggtgttttcc
    23041 cttttttctt gctcatcagt aaaactccag aatggtggat gtcagcgggg tgctgatacc
    23101 ggcggtgagt ggctcattta acagggcgtg catggtcgcc caggcgaggt cggcgtggct
    23161 ggcttcctcg ctgcggctgg cctcataggt ggcgctgcgt ccgctgctgg tcatggtctt
    23221 gcggatagcc ataaacgagc tggtgatgtc ggtggcgctg acgtcatatt ccagacagcc
    23281 acggcggata acgtcttttg ccttgagcac cattgcggtt ttcatttccg gtgtgtagcg
    23341 gatatcgcgc gcggcaggat agaacgagcg cacgagctgg aacacgccga caccgaggcc
    23401 ggtggcatca ataccgatgt attcgacgtt atatttttcg gtgagtttgc ggatggattc
    23461 agcctgggtg gcaaagtcca tgcctttcca ctggtgacgc tcaagtattc tgaatttgcc
    23521 accggccacc accggcggtg ccagtaccac gcatccggca ctgtcgccac ggtgtgacgg
    23581 gtcgtaacca atccataccg ggcgggagcc gaacggattg gcggcaaagg gtgcatagtc
    23641 ttcccattct tccagcgtgt cgaccatgca gcgttgcagc tcctcgaacg ggaacaccga
    23701 tgccttgtcg tcaacaaatt cacacatgaa caggttttta aaatcgtcgg cgctgttttc
    23761 gcgtttgagc tgctcaatgt cgaacagcgt gcagccgcct ttcagggcgt cctcaatggt
    23821 gacaatctgc cgccactggc cgtccgcaca gagaagacct ccggcaaggg cgttatgact
    23881 gacgtcgatt tccacgcgtt cggcggcgct ggcgcgtccc cggttgaaca gttcacccga
    23941 ccagaacggg taggcgtcgt gcgccagcgt ggacggggtg gagaaatagg tcgagcacag
    24001 gtggctctgt gaggccatac ctgatgccac cttacgcagt acctgaaaat tcgggatcca
    24061 gaaaatctcg tcgacgtaca ggtcgccgtt atggctctgt gcggtgttgg agttggtgcc
    24121 gagaaaaatc agttttgcgc cgttattgcc caggacaatc gggtcaccgg tcaggtcaac
    24181 gtcaaccaga cgggcaaagg cgatgatgta ttcgcggaac acatacgcct gcgttttact
    24241 ggccgacaga aaaatctggt tatgaccggt tttcagggca cgcagcagcg cctcgcggga
    24301 aaaataaaac gtcgcgccaa tctggcggga tttcaggata tctcggatgc ggtgctcaag
    24361 cccggcgcga taccagtgca actgatattc gaaagactgc tcaaagaaaa tctgctccag
    24421 cttttcgatg gcctcgtcac tgaaaaaatt ctttttcggt ttgcgacgcc cgcctttgtt
    24481 gcggttagcg acgttcggat taaggtctgc ctcgttgccg gtctgactgt agcggttgac
    24541 ccgtgccagt cgttcaatct ggcgtcccag caggtcaatt tccttgaagt caccgccggt
    24601 tttctgcggt ttgatgatga gctgggtcag ccgcgcttcc agactcattt cgacacggct
    24661 gatgggggca acgctgtccc agccgtcgcg ctgtttccag ctctgcaccg tcgggcgttt
    24721 catctgcaac atggcggcaa tctgcggcac ggaaaacccc tgccagtaca gcagcgccgc
    24781 ctgacgacgc gggtcgtgta aaagagtggt gtctgtggtg atggtcatga atacctcgcc
    24841 gtgatgaata cacggcaagg ctactgagtc gcgccccgcg attcgctaag gtgctgttgt
    24901 gtcagtgata agccatccgg gactgatggc ggaggatgcg catcgtcggg aaactgatgc
    24961 cgacatgtga ctcctctaat cactattcag gactcctgac aatggcaaaa aaagtctcaa
    25021 aattctttcg tatcggcgtt gagggtgaca cctgtgacgg gcgtgtcatc agtgcgcagg
    25081 atattcagga aatggccgaa acctttgacc cgcgtgtcta tggttgccgc attaacctgg
    25141 aacatctgcg cggcatcctg cctgacggta tttttaagcg ttatggcgat gtggccgaac
    25201 tgaaggccga aaagattgac gatgattcgg cgctgaaagg caaatgggcg ctgtttgcga
    25261 aaatcacccc gaccgatgac cttatcgcga tgaacaaggc cgcgcagaag gtctacacct
    25321 caatggaaat tcagccgaac tttgccaaca ccggcaaatg ttatctggtg gggctggccg
    25381 tcaccgatga cccggcaagc ctcggcacgg aatacctgga attctgccgc acggcaaaac
    25441 acaaccccct gaaccgcttc aaattaagcc ctgaaaacct gatttcagtg gcaacgcccg
    25501 ttgagctgga atttgaagac ctgcctgaaa ccgtgttcac cgccctgacc gaaaaggtga
    25561 aatccatttt tggccgcaaa caggccagcg atgacgcccg tctgaatgac gtgcatgaag
    25621 cggtgaccgc tgttgctgaa catgtgcagg aaaaactgag cgccactgag cagcgcctcg
    25681 ctgagatgga aaccgccttt tccgcactta agcaggaggt gactgacagg gcggatgaaa
    25741 ccagccaggc attcacccgc ctgaaaaaca gcctcgacca caccgaaagt ctgacccagc
    25801 agcgccgcag caaggccacc ggcggtggcg gtgacgccct gatgacgaac tgctgaccgg
    25861 cgtcagtcag tccggaaaaa ccttcacgat taacccttaa tttcaggaaa aactatgcgc
    25921 caggaaaccc gctttaaatt taatgcctac ctgtcccgtg ttgccgaact gaacggcatc
    25981 gacgccggtg atgtgtcgaa aaaattcacc gttgaaccgt cggtcaccca gaccctgatg
    26041 aacaccatgc aggagtcctc tgatttcctg acccgcatca acattgtgcc ggtcagcgaa
    26101 atgaaagggg aaaaaattgg catcggtgtc accggctcca tcgccagcac caccgacacc
    26161 gccggtggca ccgagcgtca gccgaaggac ttctcgaagc tggcgtcaaa caagtacgaa
    26221 tgcgaccaga ttaacttcga tttttatatc cgctacaaaa cgcttgacct gtgggcgcgt
    26281 tatcaggatt tccagctccg tgtccgtaac gccattatca aacgccagtc ccttgatttc
    26341 atcatggccg gttttaacgg cgtgaagcgt gccgaaacct ctgaccgcag cagcaatccg
    26401 atgctgcagg atgtggcggt cggctggctg cagaaatacc gcaatgaagc cccggcgcgc
    26461 gtgatgagca aggtcactga cgaggaaggg cacaccacct ctgaggtcat ccgcgtgggt
    26521 aagggcggtg attatgccag cctcgatgca ctggtgatgg atgcgaccaa caacctgatt
    26581 gagccgtggt atcaggaaga ccctgacctt gtggtgattg tggggcgtca gctgctggcg
    26641 gacaaatatt tccccatcgt caacaaggag caggacaaca gcgaaatgct ggccgctgac
    26701 gtcatcatca gccagaaacg catcggtaac ctgccggcgg tacgcgtccc gtacttcccg
    26761 gcggatgcga tgctcatcac gaagctggaa aacctgtcca tctactacat ggatgacagc
    26821 catcgccgcg tgattgagga aaacccgaaa ctcgaccgcg tggagaacta cgagtcaatg
    26881 aacattgatt acgtggtgga agactacgcc gccggttgtc tggtggaaaa aattaaggtc
    26941 ggtgatttct ccacaccggc taaggcgacc gaagagccgg gagcgtaacc gatgacgagt
    27001 cccgcacagc gccacatgat gcgggtctcg gcagcgatga ccgcgcagcg ggaagccgcc
    27061 ccgctgcgac atgcaactgt ctatgagcag atgctggtta agctcgccgc agaccagcgc
    27121 acactgaaag cgatttattc aaaagagctt aaggccgcga aaaaacgcga actgctgccg
    27181 ttctggttgc cgtgggtgaa cggcgtgctg gagcagggca aaggtgcaca ggatgacatt
    27241 ctgatgacgg tcatgctgtg gcgtctggat accggcgata ttgccggtgc gctggagatt
    27301 gcccgttatg ccctgaagta cggtctgacc atgccgggta aacaccgccg caccccgccg
    27361 tacatgttca ccgaggaggt ggcgcttgcg gccatgcgcg ctcacgctgc cggtgagtct
    27421 gtgaatcccc gcctgctgac ggacaccctt gaactgaccg ccacggctga catgcctgat
    27481 gaagtgcgcg caaagctgca caaaatcacc ggtctgtttc tgcgtgacgc tggtgatgcc
    27541 gcaggggcgc tggcgcacct gcaacgtgcg acacagctcg actgtcaggc aggcgtcaaa
    27601 aaagagattg aacgactgga gcgggagctg aaaccgaagc cggagccgca gccaaaagcg
    27661 gtcacccgcg ccccgcgtaa gacccggagc gtgacaccgg caaaacgtgg acgcccgaaa
    27721 aagaaagcca gttaacaacc gaatgcgccc cgcgccaggg cggcacgccg gtcagtgagg
    27781 gtgaatcacc tgacactgca ccggcgtcca ccgcccgact tttcagaggt agtcatgatg
    27841 acgctgatta ttccgcgaaa ggaggctccc gtgtccggtg aggggacggt ggtcatcccg
    27901 caaccggcag gcgacgagcc ggtgattaaa aacacgttct tttttcccga tatcgacccg
    27961 aagcgcgtcc gggaacgtat gcgccttgag cagaccgtcg cccccgcccg tctgcgtgag
    28021 gccatcaagt caggcatggc ggagacgaat gcggagctgt acgaataccg cgaacagaaa
    28081 attgccgccg gttttacgtg tctggcggac gttccggcgg acgacatcga cggtgaaagc
    28141 atcaaagttt tttactacga gcgcgccgtg tgtgcgatgg cgaccgcatc gctttatgag
    28201 cgttatcgcg gcgtggatgc cagtgcgaaa ggcgacaaga aggctgacag cattgacagc
    28261 accattgatg agctgtggcg ggatatgcgc tgggcggtgg cgcgcatcca ggacaagccg
    28321 cgctgcatcg tgagtcaaat ctgatgaaga cctttgcgct acagggcgac acgctcgacg
    28381 ccatttgtgt ccggtattac gggcgcactg agggcgtggt tgagaccgtg ctcgccgcaa
    28441 atccgggact ggctgaactg ggtgtggtgc tgccacacgg caccgccgtc gaactgcccg
    28501 atgttcagac cgcgcccgtg gctgaaactg tcaatctgtg ggagtaacgc atgacagcag
    28561 aagaaaaaag cgtcctgtcg cttttcatga ttggggtgct gattgttgtc ggcaaggtgc
    28621 ttgccggtgg tgaacccatc accccgcgtc tgtttatcgg gcgcatgttg ctcggtggtt
    28681 ttgtctcgat ggttgccggt gttgttctgg tgcagtttcc tgacctgtca ctgcctgcgg
    28741 tgtgcggcat cggctccatg ctgggtatcg ccggttatca ggtgattgag attgccattc
    28801 agcgccgctt taagggcagg gggaaaccgt aatgccggta attaacacgc atcagaatat
    28861 cgccgccttt ctcgacatgc tggcagtgtc cgaagggacg gcgaatcatc cgctgacgaa
    28921 aaaccggggc tatgacgtga tagtcaccgg actggacggg aagccggaaa ttttcaccga
    28981 ctacagtgac cacccgttcg cgcatggccg accggcgaag atgtttaacc gtcgcggtga
    29041 aaaatccacg gcctccggtc gctatcagca gctttacctg ttctggccgc attaccgcaa
    29101 acagcttgcc ctgccggatt tcagtccgtt gtcacaggac aggctcgcca ttcagttgat
    29161 ccgcgaacgc ggtgcgctgg atgacatccg ggcgggacgc attgagcgcg ccatttcacg
    29221 ctgtcgcaat atctgggcgt ccctgccggg tgccggttac ggtcagcgtg agcattcact
    29281 ggaaaaactg gtcaccgtct ggcgtaccgc tggcggcgta ccggcttaaa cggagtaaac
    29341 accatgaaga aattatccct ttcactgatg ctgaacgtgt cgctggcgct gatgccggca
    29401 ctgtccctga tttacccgca gagcgtggcc gtcagttttg tcgccacctg ggcgattctg
    29461 gcgatggtta tctgtgtggt tgccggtggt gtcggcgtgt atgccactga gtatgtgctg
    29521 gaacgctacg ggcgggagct gccgccggaa tcgctggccg tgaagattgt cacgtcgctg
    29581 tttttgcagc cggtgccgtg gcgcagacgg gcggcggctc tgatggtgat ggtggcgacg
    29641 tttatctcgc tggtcgctgc cgggtggatt tttaccgcgc tgatttatct cgtggcgtcg
    29701 ctgttcttcc ggctgatatg tacggcctgc cgtcagcgtc ttgaggagcg ggaaccatgt
    29761 caaagctgat gattgtgctg gtcgtgttgt tatcgctggc ggtgtccggt ctgtttctgg
    29821 tgaaacacaa aaatgccagc ctgcgcgcct cgctggacag ggcgaataac gtcgccagtg
    29881 aacagcagac gaccatcacc atgctgaaaa atcagcttca tgttgccctc accagggcag
    29941 acaaaaacga gctggcgcag gtggcactgc gtcaggaact ggaaaacgcc gcgaagcgtg
    30001 aagctcagcg cgagaaaacc atcacgagat tactcaatga aaacgaagat tttcgccgct
    30061 ggtacggtgc tgacctgcct gatgctgtgc gccggttgca ccagcgcccg gcctgcgcag
    30121 acgccagtga ttgtccacaa cgcctgcccg aaagtgagtc tttgcccgat gccgggcagt
    30181 gacccgcaga agaacggcga tttaagtgcc gatatccggc tgcttgagaa cgcgctggca
    30241 cgctgtgcca gccaggtaaa aatgattaaa cactgtcagg acgaaaacga tgctcaaacc
    30301 cgacagcctg cgcagggcgc tgactgatgc cgtcacggtg ctgaaaacta accccgatat
    30361 gctgcggata ttcgtggata acgggagtat tgcctccaca ctggcgacgt cgctgtcatt
    30421 cgaaaagcgt tacacgctca atgtgattgt gaccgacttt accggtgatt ttgacctgct
    30481 cattgtgccg gtgctggcgt ggctgcggga aaatcagccc gacatcatga ccaccgacgc
    30541 aggccagaaa aagggcttca cgttttatgc ggacatcaac aatgacagca gctttgatat
    30601 cagcatcagc ctgatgctga ccgagcgaac gctggtcagt gaggtggacg gcgcactgca
    30661 tgtgaagaat atcccggaac ccccgccgcc ggagccggtc acccgcccga tggagcttta
    30721 tatcaatggc gaactggtga gcaagtggga tgaatgagtt taagcgtttt gaagaccggc
    30781 tgaccggact gattgagtcg ctgtcaccgt cagggcgtcg gcggctgagt gcagagctgg
    30841 cgaagcgtct gcggcagagt cagcagcgtc gggtgatggc acagaaagcc ccggacggca
    30901 caccctacgc gccacgccag cagcagagcg ccagaaaaaa gaccggtcgt gttaagcgaa
    30961 aaatgtttgc gaaacttatc accagtcgtt ttttgcatat ccgcgccagc ccggaacagg
    31021 catcaatgga attttacggc gggaagtcgc cgaaaatcgc cagtgtgcat cagttcggtc
    31081 tgtcggaaga aacccggaaa gacggtaaga aaattgatta tccggcgcgc cctctgctcg
    31141 gcttcaccgg tgaggatgta cagatgattg aagagattat cctggctcac ctcgaccgtt
    31201 agttgtgcca ttcccgacac ctcatcgtca cattgccgcc ggtatgaccc ggcggcatcc
    31261 ttcccgttat gaacactctc gcaaatatcc aggaactcgc gcgcgcactg cgcaacatga
    31321 tacgccccgg cattatcgtc gaaaccgacc ttaacgccgg tcgctgccga gtgcagaccg
    31381 gcggcatgtg cactgactgg cttcagtggc tgacccatcg tgccggtcgt tcgcgcacat
    31441 ggtgggcacc ttccgtgggg gagcaggtgc tgattctggc cgtgggcggt gaactcgaca
    31501 cggcgttcgt tctgccgggg atttattccg gcgataaccc cgcgccgtct gcgtcggcgg
    31561 atgccctgca tatccgtttc cctgacgggg cggtgattga gtatgaaccc gaaaccagtg
    31621 cactcacggt aagcggaatt aaaacggcca gcgtgacggc ttctgattct gttactgcca
    31681 cggtgccggt ggttatggtg aaagcatcaa cccgcgtcac cctggacaca ccggaggtgg
    31741 tctgcaccaa caggctgatt accggcacgc tggaagtgca gaagggcggg acgatgcgcg
    31801 gcaacattga acacaccggc ggtgaactct catcaaacgg taaggtactg catacccaca
    31861 aacacccagg cgacagcggc ggcacaaccg ggagtcctct atgacagcgc gttatctcgg
    31921 aatgaatcgc agtgatggcc tgactgtcac tgaccttgag catatcagcc agagtatcgg
    31981 cgatatcctg cgcacaccgg tcggctcacg ggtgatgcgt cgtgattacg gctcgttgct
    32041 ggcgtcaatg attgaccagc cgcagacccc ggcgcttgag ttgcagatta aggtcgcatg
    32101 ttacatggcg gtgctgaaat gggaaccccg cgtcacgctg tcatctgtca ccacggcgcg
    32161 cagttttgac gggcgaatga cggtcacgtt aaccggccag cacaacgaca ccggccagcc
    32221 actttcgtta accatccctg tgagttgaaa ccatgccgat tatcgacctg aaccagctac
    32281 ccgcaccgga tgtggtcgag gagctggact ttgaaaccat tctcgccgaa cgcaaggcga
    32341 cactgatttc cctttacccg gaagaccagc aggaggcggt cgcccgtacc ctgacgctgg
    32401 aatctgagcc tctcgtcaaa ctgctggagg aaaatgctta tcgtgagctt atctggcgtc
    32461 agcgtgtgaa tgaggctgca cgggcggtga tgctggcctg tgcagccggt aatgaccttg
    32521 atgtgattgg tgccaattac aacaccacgc gcctgattat caccccggca gatgattcga
    32581 ccatcccgcc gacaccggca gtgatggaat ctgacaccga ttatcgtctg cgtattcagc
    32641 aggcgtttga aggtttaagc gtcgccgggt cggtgggtgc ctatcagtat catggccgca
    32701 gtgccgacgg gcgtgtcgcg gatatctctg tcaccagtcc gtctccggcc tgcgtcacca
    32761 tctctgtgct gtcacgtgag aataacggtg tggcatccga agacctgctg gctgtggtac
    32821 gtaacgccct taatggcgag gatgtcaggc cggtggccga ccgcgtgacc gtgcagtctg
    32881 ccgccatcgt tgaataccag ataaacgcca cgctttacct ttaccctggt cccgaaagcg
    32941 aacccatccg cgcggccgca gtaaaaaaac tggaagcgta tatcacggca cagcaccggc
    33001 tggggcgcga catccgtctg tctgccattt atgccgcttt gcatgtggaa ggcgtgcagc
    33061 gtgtcgaact gactgcacca ctggctgaca tcgtgctcaa cagtacgcag gcgtctttct
    33121 gtaccgaata ccgcgtcgtg accggaggct cggatgagtg attcgcgact gctgccgacc
    33181 ggctcatcac cgcttgaagt tgctgccgca aaagcctgtg cggaaattga aaaaacgccg
    33241 gtcagtattc gtgagctgtg gaacccggat acctgtccgg caaatctgct gccgtggctg
    33301 gcgtgggcgt tttcggtcga caggtgggat gaaaagtggc cggaagcgac aaaacgcgcc
    33361 gttatccgcg atgcgtattt cattcattgt cataaaggca ctgtcggcgc aatccggcgt
    33421 gtggtggagc cgctcggcta tctcattaac gtaaaggaat ggtgggagac aaacgacccg
    33481 cccggaacct ttcgccttga tatcggtgtg ctggaaagcg gtatcacgga ggagatgtat
    33541 ctggaaatgg aacggctgat tgccgatgcc aaacccgcaa gtcgccatct tatcggtctg
    33601 aacattatcc aggacattcc cggctatctg tatacaggcg gtgtggtctg tgatggtgat
    33661 gttattactg tttatcccgg ataagtgaga aacaatgagc acgaaattta aaaccgttat
    33721 cactactgcc ggagccgcaa agctggcagc cgccactgtc cccggcggga aaaaagtaaa
    33781 cctgtctgca atggccgtgg gtgacggtaa tggcaaattg ccggtgccgg atgccggtca
    33841 gacgaaactg gtgcatgagg tctggcgtca cgctctgaat aaagtcagcg tggataacaa
    33901 gaataaaaac tatatcgtgg ctgaactggt tgtaccgccc gaagtgggcg gcttctggat
    33961 gcgtgagctt ggtctgtatg acgatgccgg aacactgatt gcggtatcca acatggcaga
    34021 aagctataag ccagaactgg ctgaaggctc cggacgtgcg cagacctgcc gcatggttat
    34081 tattctcagc aacgtggcgt ccgttgagct gagtattgat gccagcacgg tgatggcgac
    34141 gcaggattac gtcgatgaca aaatcgcaga gcatgagcag tcccgccgcc atcctgacgc
    34201 cacgctgaca gaaaaaggtt ttactcagtt aagcagtgca acaaacagca ccagtgaaaa
    34261 gctggcggca acgccaaaag cggtcaaggc agcaaatgac aacgcaaatt cacgtctggc
    34321 gaaaaatcag aacggtgcag atatccagga taaatcagct tttctggaca atattggcgt
    34381 taccagcctg acgtttatga aacacaatgg aatgattcca accactgaca atcttgactc
    34441 ctatggccca gaagaaaaat atcttggaac gtggtcatgt ccgtcacaat ccactgcaaa
    34501 accagagtct ggttaccctg aagataaagg caatggggtt ctggaagtat tcaatgctgg
    34561 tcgctttcac tgcacacaac gctacaccac ccgaaccggt aatatttaca ttcggatgct
    34621 tgatgcagaa tggaacccag ccagtccaac atggagtgca tggagagtta ttacaagcgg
    34681 gactcgacca ttaagcacgt caattgacct gaattcttta ggtggcgcgg aacacctggg
    34741 aatatggcgc aatagcagta catcaatagc ctcatttgaa cgtcatttcc cggaggacgg
    34801 gagctttggt cagggtattc tggaagtctt tgaaggcggt ctgtatggga gaatgcaacg
    34861 ttacaccacc cgtagtggga ctatgtacat tcgcggactg actgcctcat gggatgcaga
    34921 gaatccacaa tgggaggact ggattgctgt tgggtatcag tccacgggat ggacttattc
    34981 aggggatctt gatgatcttt taaagcctgg tatttatagc gtcacgaaac aggccacaaa
    35041 tgcaccagtc accgactcta aagatcttgc tgttggctca atagttgaag tgaaaaaacg
    35101 ttgtgatatt gaatcgtata tccagacata tacgacagtt tctgcaacgg atgcctataa
    35161 aaaccggaca tttcaacgaa ctcgtgccag cggtgaagct gactgggggg aatgggcgga
    35221 agtttacaac tcaaaatcat tactcactaa attaggtgtc ggcggtgtta ccgacaggtt
    35281 atccagtctg gactggcaga cctacgactt tgtgccgggc agcatgataa ctgtcagact
    35341 ctccgatatg accaatatcc ccgatgggat ggaatggggc gtcattgata ctaacctgat
    35401 aaatatcact gttggcccca gtgaaggtgg aggtgtcgcc cgttcaatgc aggtatggcg
    35461 cagcacctca aacaaaacca actatcgttt ttttacagtc cgcttatatg gcaatcctgg
    35521 ggagcgtagc ttcaatattc gtcggctacc aattatcgac gaagcccaga catgggaggc
    35581 aaaacagaca ttcagtgcag gcctctctgg tgaactgtcc ggcaatgcgg cgacagcaac
    35641 aaagctgaaa acagcccgta aaattaataa cgtttcgttt gatggaacat cagatattaa
    35701 cctgacgccg aaaaatattg gtgcatttgc ttcaggaaaa acaggagaca ccgttgcgaa
    35761 tgataaagcc gttggatgga actggagtag cggagcctat aacgcaacta ctggtggggc
    35821 atcaacgtta attcttcatt ttaatatcgg tgaaggaagt tgtcccgcag cccagttccg
    35881 cgttaattat aagaacggcg gtatttttta tcgttctgct cgtgatggtt acggattcga
    35941 ggctgactgg tctgagtttt ataccacaac gcgaaaacct acagcgggag atgtcggtgc
    36001 actgtcgtta tctggtggtc aattgaatgg tgcactgggc atcggaacat ccagtgctct
    36061 tggcggtaat tcgattgttt tgggtgataa tgacacgggc tttaaacaaa atggtgatgg
    36121 taatctggat gtttatgcta atagcgtcca tgttatgcgc tttgtctccg gaagcattca
    36181 aagtaataaa accataaata ttacggggcg tgttaatcct tcggattacg gtaactttga
    36241 ttcccgctat gtgagagatg tcagacttgg cactcgtgtt gttcagacca tgcagaaagg
    36301 cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattgtcg gtgaagtcga
    36361 tggtgatgac cccgcagtat tcagaccaat acaaaaatac atcaatggca catggtataa
    36421 cgttgcgcag gtgtaaatta tgcagcattt aaaaaacatt aagtcaggta atccaaaaac
    36481 aaaagaacaa tatcagctaa caaagaattt tgatgttatc tggttatggt ccgaagacgg
    36541 aaaaaactgg tatgaggaag taagtaattt tcaggaagac acgataaaga ttgtttacga
    36601 cgagaataat ataattgtcg gcatcaccag agatgcttca acgttcaacc ctgaaggttt
    36661 tagcgttgtc gaggttcctg atattaccgc caaccgacgt gctgatgact caggtaaatg
    36721 gatgtttaag gatggtgccg tgattaagcg gatttatacg gcagacgaac agcaacaaca
    36781 ggcagaatca caaaaggccg cgttactttc cgaagcggaa agcgttattc agccactgga
    36841 gcgcgctgtc aggctgaaca tggcaacaga tgaggagcgt agccgactgg aagcatggga
    36901 acgctacagt gttctggtca gccgtgtgga tcctgcaaat cctgaatggc cggaaatgcc
    36961 gcaataagtt gtatgagctg acatatctat agcacagagt aaagcctaat ctgacagtcc
    37021 gctctgtgca aggagcggac attacctaac acattggtaa ccttcacaga tttacgcgtt
    37081 ctacatagtt agcattgtat ctgatgagtt atcatgtttt aaaatatcca actgataaat
    37141 ccacctgaat acttttcact aattttgatt gcataaagat catgaagagt tataaataaa
    37201 tcattatctt ctttgaaaat taactcctca tactctgcca ttatcaaatt atgctgtgtt
    37261 gttagtttag atatgttatc tatttcactg gcaagcatgg atttgtttac attggtgtgt
    37321 ggatccatgg caatatattt ccgcatcaat ggaattagtt gatcagggtc tatatttttt
    37381 gcattttcct ttgctttttt ttgtctttct gaatagatgg agttaattgt gttagtgtgt
    37441 tttttcgcca gtctcttaaa ctctggatat gaaccagtaa ctaatgcaag ctcttcgtac
    37501 ttaaggaata aatcgatatt gcattttttt accgtcattt caagatcttc aactcttttt
    37561 tttaactctt catttgtttt aataagagac atcgggagaa cttcataatg ttgtcctaat
    37621 tctgaatcag ggctcattgc catatgtact atttcattat acatgagctg ataagaagca
    37681 gataactctc catactttag aaatagttca ttatgtgaag atgaacaaga actttgtaat
    37741 ttcatcattg gcctaaagta atcctttata aactcctcct gaagatcttg gtcttttttg
    37801 tagctctcta ggattccagc aaaaagaaat gaccctaaag atatagagat tatggatatt
    37861 agcaattgac taaggaatga ttgtgtttca ttactcataa aacatcctta aaaaataaca
    37921 cgaaaagtga ctcctgacag ggaagaaacg acaggtcgga aacaaactta actgatgagt
    37981 tagctgtagt ccagaacctt gttttaacac ttagtgtgac tgtttgcatc tcaggaattt
    38041 gtgccaataa tatttgaatc cttataattt atttaaatca aatttagtta ctcgctcaaa
    38101 gcaggctgtc agatttgata gcgtttgggc tatgtaaact gtcagtcgga aaatgagtgt
    38161 gtacaaatca ggacaggcga gctgattgcc cgccttttct ttatctgttg tttcatccac
    38221 tgaccagcca ggtcaaatag cgtctcatgc tctgcacaac agaaaatagt tgcacccatt
    38281 aaccacggag ttaaacggat gagtgactat catcatggcg tgcaggtgct ggagattaac
    38341 gacggcaccc gcgtcatttc caccgtatcc actgccattg tcggcatggt ctgcacggcc
    38401 agcgatgcgg atgcggaaac cttccctctc aataaaccgg tgctgattac caatgtgcag
    38461 agcgcaattg caaaggccgg taaaaaaggc acgctggcgg cgtcgttaca ggccatcgct
    38521 gaccagtcaa aaccggtcac cgttgttgtg cgcgtggaag acggcaccgg cgacgacgag
    38581 gaaacgaaac tcgcgcagac tgtttccaat attatcggca ccaccgacga aaacggtcag
    38641 tacaccggac tgaaagccct gatgggggct gagtcggtta ccggcgttaa accgcgcatt
    38701 ctcggcgtac cgggactgga caccaaagag gtcgccgtcg cactggcatc ggtatgccag
    38761 gaactgaatg ccttcgggta tatcagcgca tggggttgta aaaccatttc cgaggtgaaa
    38821 gcctaccgcc agaatttcag ccagcgtgag ctgatggtta tctggccgga tttcctcgca
    38881 tgggatacgg ttaccagtac caccgccacc gcgtatgcca ccgctcgtgc gctgggtctg
    38941 cgcgctaaaa tcgaccagga gcagggctgg cataaaacgc tgtccaacgt cggggtgaac
    39001 ggtgttaccg gcatcagtgc atccgtcttc tgggatttgc agaagccagg cactgatgct
    39061 gacctgctga acgaggcagg cgtcacaacg ctggttcgcc gcgacggttt tcgtttctgg
    39121 ggtaaccgta cctgctctga tgacccgctg ttcctctttg aaagctacac acgcaccgcg
    39181 caggtactgg ccgacacgat ggctgaggcg cacatgtggg ctattgataa gcccatcacc
    39241 gcaacgctga ttcgcgacat cattgacggc atcaatgcca aattccgtga actgaaaaac
    39301 aacggttata tcgtggatgg cacatgctgg ttcagcgaag aatccaacga tgcggaaacc
    39361 ctcaaggccg gaaaactgta tatcgactac gactataccc cggtgcctcc tctcgaaaac
    39421 ctgaccctgc gccagcgtat tacttccaga tacctggcaa gtctggtcac ttcggttaac
    39481 agcaattaag gagcctgacc gatggcaatg ccgcgcaaac tcaagttaat gaacgtcttt
    39541 ctgaacggct acagctatca gggcgttgca aagtccgtca cgctgccaaa actgacccgt
    39601 aagctcgaaa actatcgcgg tgcggggatg aacggcagcg caccggtaga cctcggcctt
    39661 gatgatgatg ctctgtcaat ggagtggtcg ctcggtggat tcccggattc ggttatctgg
    39721 gagctttacg ctgcaaccag tgctgatgcc gtaccgattc gttttgctgg ttcttaccag
    39781 cgcgacgata ccggcgaaac ggtggccgtc gaagtggtca tgcgtgggcg tcagaaagaa
    39841 gtcgataccg gagagggtaa acagggagag gataccgaat cgaaactccc ggtgatttgt
    39901 acttatttca agttgacgat ggacggtaag gagctggtcg aaatcgacac catcaacatg
    39961 attgagaagg tgaacggcgt cgaccggctg gagcaacacc gccgcaatat cggcctgtga
    40021 ttttcatccg ggtcagcctg gctgaccggt taaccccgat tcagaagtga gaaaaccatg
    40081 aacaaagaaa atgtgattac cctggacaat ccggtcaagc gtggtgagca ggttatcgaa
    40141 caggtcacgc tgatgaaacc taatgccggg acgctgcgcg gtgtcagtct ggctgcggtc
    40201 gcaaactccg aagtcgatgc actgattaag gtgctgccgc gcatgacggc accgatgctg
    40261 accgagcagg aggtcgccgc gctggaactg cctgaccttg tggcgctggc cggtaaggtg
    40321 gtcggttttt tgtctccgaa ctcggtgcag tgacgtttcc gaaaaatctg tcggtcgatg
    40381 acctgatggc agatgtggca gtgatatttc actggccgcc atcagaactg tatcccatga
    40441 gcctgaccga actcatcaca tggcgcgaaa aggcgctccg gcgaagcgga agcacgaatg
    40501 agtaacaatg taaaattaca ggtattgctc agggctgttg accaggcatc ccgcccgttt
    40561 aaatccatcc gtacagcgag taagtcgctg tcgggggata tccgggaaac acaaaaatca
    40621 ctgcgcgagc tgaacggtca cgcatcccgt attgagggat ttcgcaagac cagcgcacag
    40681 cttgccgtga ctggtcatgc acttgaaaag gcacggcagg aagccgaagc acttgccaca
    40741 cagtttaaaa acactgaacg tccgacccgt gctcaggcga aagtgctgga atccgcaaag
    40801 cgtgcggcgg aggacttaca ggcgaaatat aaccgcctga cggattccgt taagcgccag
    40861 cagcgggaac tggccgctgt gggaattaat acccgcaatc ttgcacatga tgagcaggga
    40921 ctgaaaaacc gtatcagtga aaccaccgca cagcttaacc ggcagcgtga cgcgctggcg
    40981 cgtgtcagtg cgcaacaggc aaaacttaat gcagtaaaac agcgttatca ggccggaaag
    41041 gaactggccg gaaatatggc ctcagtgggc gctgccggtg tggggattgc ggcggcggga
    41101 acgatggccg gagttaagtt gctgatgccc ggttatgagt ttgcgcagaa aaactcagaa
    41161 ttgcaggccg tgctgggtgt ggcaaaagac tccgccgaaa tgaccgcact acgcaaacag
    41221 gcgcgccagc tcggcgacaa taccgccgcc tcggcggatg atgcggccgg tgcacagata
    41281 atcatcgcga aagcgggtgg ggatgttgat gccattcagg cggcaacgcc ggtcacgctg
    41341 aatatggcgc tggcgaaccg ccgcacgatg gaagaaaacg ccgccctgct gatggggatg
    41401 aaatccgcct ttcagctttc aaacgataag gtcgctcata tcggggatgt tctctccatg
    41461 acgatgaaca aaaccgccgc cgattttgac ggcatgagcg atgcgctgac ctatgccgca
    41521 cctgtggcaa aaaatgccgg tgtcagcatt gaagaaaccg ccgcaatggt cggggcgctg
    41581 catgatgcaa aaatcacagg ctcaatggcg gggacgggaa gccgtgccgt gttaagccgc
    41641 ctgcaggcac cgacgggaaa agcatgggat gcactcaaag agcttggagt gaaaacctca
    41701 gacagcaagg gaaacacccg gccaatattt accattctga aagaaatgca ggccagtttt
    41761 gagaaaaacc ggctcggtac tgcccagcag gctgaataca tgaaaaccat tttcggggag
    41821 gaggccagct cagccgccgc cgtgctgatg actgccgcct caaccggaaa gctggacaaa
    41881 ctgaccgctg cgtttaaagc ctcggacggg aagacctcag agctggtaaa tattatgcag
    41941 gacaatctcg gcggtgactt taaggagttt cagtccgctt atgaggcggt ggggactgac
    42001 ctgtttgacc agcaggaagg cgcactgcgt aagctcacgc agacggccac aaagtatgtg
    42061 ttaaaactcg acggctggat acagaaaaac aaatcactgg cgtcaaccat cggcatcatt
    42121 gccggcggtg cactggtgct gactggcatc atcggtgcca ttggccttgt agcttggccg
    42181 gttatcaccg gcatcaatgc cattatcgcg gcagcaggcg caatgggggc aatcttcacg
    42241 acggttggta gtgccgttat gacggccatc ggtgcgatta gctggccggt tgtggccgtg
    42301 gtggccgcta ttgtcgccgg ggcgttactt atccgtaaat actgggagcc tgtcagcgca
    42361 ttctttggcg gtgtggttga agggctgaaa gcggcatttg cgccggtggg ggaactgttc
    42421 acgccactta agccgctgtt tgactggctg ggtgaaaagt tacaggccgc gtggcagtgg
    42481 tttaaaaacc tgattgcccc ggttaaagcc acccaggaca cactgaaccg ttgccgtgac
    42541 acgggcgtca tgttcgggca ggcactggct gacgcgctga tgctgccgct taatgcgttc
    42601 aacaaactgc gcagcggtat tgactgggta ctggaaaaac tcggtgttat caacaaagag
    42661 tcagacacac ttgaccagac cgccgcaagg actcatgccg ccacgtatgg caccggtggt
    42721 tatattccgg cgaccagctc ttatgcaggc tatcaggctt atcagccagt cacggcaccg
    42781 gctggccgct cttatgtgga ccagagtaaa aacgaatatc acatcagcct gacgggtggt
    42841 actgcgccgg ggacacagct tgaccgccag ttacaggatg cgctcgaaaa atacgagcgg
    42901 gataaacgtg cgcgcgcccg tgccagcatg atgcatgacg gttaaggagg tgacgaaaaa
    42961 tgatgctcgc gttaggtatg tttgttttta tgcgccagac gctgccacac cagaccatgc
    43021 agcgtgaatc ggattatcgc tggccgtcaa attcccgtat cggtaaacgg gatgcctttc
    43081 agtttctcgg tgtgggtgag gaaaacatca cgctggccgg tgtgctttat cccgaactga
    43141 ccggcggaaa gctgacgatg accacgctca gactgatggc agaggaaggt cgggcgtggc
    43201 cgttgctgga tggcaccggc atgatttacg gcatgtatgt catcagcagg gtgagtgaaa
    43261 cagggagtat tttctttgca gacggcacac cccggaaaat tgattttacg ctgtcgctca
    43321 cccgcgttga tgaatcactg gccgcgcttt atggcgatat cggtaaacag gcggaatcgc
    43381 tcatcggtaa ggctggcagt atggagacca gattcacggg tatgacgggg gcgggataat
    43441 gctggatacg ctgacatttg atgcaggcag tacgctgacg ccggattaca tgctgatgct
    43501 cgacagcagg gatattaccg gcaatatcag cgatcgactg atgagcatga ccctgacgga
    43561 taaccggggc tttgaggctg accagcttga tattgaactg aacgatgccg acgggcaggt
    43621 cgggctgccg gttcgtggcg ctgtcctgac ggtgtatatc ggctggaaag gttttgccct
    43681 ggtatgcaaa gggaaattta ccgttgatga ggttgaacac cggggcgcac cggatgtggt
    43741 caccatccgc gcccggagtg cagattttcg cgggacgctc aattcccgcc gtgaaggctc
    43801 ctggcatgac accacgctcg gtgcgattgt tgaggcgata gcctcccgta acaggctgga
    43861 agccagtgtc gctccgtcac tggccggaat taaaatcccg cacatcgacc agtcgcagga
    43921 gtctgatgcg aaattcctga cccgtcttgc agaacgcaac ggcggtgagg tgtcggtaaa
    43981 aatgggaaaa ctgttgtttc tcaaagcggg gcagggggtg acggccagcg gtaaaaaaaa
    44041 cccgcaggtc accataaccc gcagcgacgg cgaccgccat cattttgcga ttgctgaccg
    44101 tggagcctat accggcgtaa cggcaaagtg gttacacacc aaagacccga agccacaaaa
    44161 gcagaaggta aaactgaaac gcaaaaagaa agagaaacac ctgcgcgcac tggagcaccc
    44221 gaaagcgaaa ccagtcacgc agaagaaagc gccaaaagta ccggaagcgc gcgaaggtga
    44281 atacatggct ggtgaggctg acaacgtttt tgccctgacc acggtatatg ccacgaaagc
    44341 gcaggccatg cgcgccgctc aggcgaagtg ggataaactg caacggggcg ttgcggagtt
    44401 ctccatcagc cttgccactg gtcgtgctga tatttacacg gaaacaccgg tcagagtgtc
    44461 aggctttaag cgcgtcatag acgagcagga ctggacaatc actaaggtga cacattttct
    44521 gaataatagt ggcttcacga cgtccttaga gcttgaggtc aggctttctg atgtggagta
    44581 cgaaacagaa gatgatgatt aatgttttgt tttatctgtt tgttttataa ggataaatta
    44641 aataaaatgg caccattaac aaaaccggaa gaggtgctcg cgatgtttca ttgtccttta
    44701 tgccagcatg ccgcacatgc gcgtacaagc cgctatatca ctgacacgac aaaagagcgt
    44761 tatcaccagt gccagaacgt gaattgcagc gccacgttca tcacttatga gtcggtacag
    44821 agatacatcg tgaagccggg agaagtccac gccgtggggc cgcacccgtt gccgtcaggg
    44881 cagcaaatta tgtggatgtg atcacaaaaa tagcccctca gtcgaggggc ttatttatgg
    44941 tcgatgtgga cgctatgtgg acagtggttg acataaatcc atttatatca tcaggttagg
    45001 tgcttttttg tgacaccatc cctgtcttcc cccacatgat gtgggggttt tttttatcct
    45061 caatttgcct gctgcttaat gcattgcaga tgatttgctt ccgttatact accgtcagtt
    45121 gatagcggga gtatttatga atcaatctta tggacggctg gtcagtcggg cggcgattgc
    45181 tgcgacggcg atggcttcgc tgctattgct gattaaaatt tttgcatggt ggtataccgg
    45241 gtcggtgagt attctcgccg cgctggtgga ttcgctggtg gatatcggcg cgtcgttgac
    45301 gaatttactg gtggtgcgat attccctgca acctgccgac gataatcact cgtttggtca
    45361 cggtaaagct gagtccctcg cggcgctggc gcaaagtatg tttatctccg gttcggcact
    45421 attcctgttt ttgacgggta ttcaacatct ggtatctcca acaccgatga cagatccagg
    45481 cgtcggggtt atcgtgacaa ttgtggcgct aatttgtacg attatccttg tctcgtttca
    45541 gcgttgggtg gtgcgccgga cgcaaagcca ggcggtgcgg gctgatatgc tacattacca
    45601 gtctgatgtt atgatgaacg gcgcaattct gctggcgctg gggttgtcct ggtacggctg
    45661 gcatcgcgcc gatgctctgt ttgcattggg aatcggcatc tatattttat atagcgcgtt
    45721 acgcatggga tatgaggcgg tacagtcatt actggatcgc gcattacctg atgaggaacg
    45781 gcaagaaatt attgatatcg tgacttcctg gccgggtgtt agcggcgctc acgatcttcg
    45841 cacgcggcag tcagggccga cccgctttat tcagattcat ttggaaatgg aagactctct
    45901 gcctttggtt caggcacata tggtggcgga tcaggtagag caggctattt tacggcgttt
    45961 tccgggatcg gatgtgatta tccatcagga cccctgttcc gtcgtaccca gggagggtaa
    46021 acggtctatg ctttcataat cagtataaaa gagagccaga cccgcatttt gtgtataaaa
    46081 taccgccatt tggcctgacc tgaatcaatt cagcaggaag tgattgttat actatttgca
    46141 cattcgttgg atcatttcga tgtgcaagaa gacttccggc aacagatttc attttgcatt
    46201 ccaaagttca gaggtagtca tgattaagaa aatcggtgtg ttgacaagcg gcggtgatgc
    46261 gccaggcatg aacgccgcaa ttcgcggggt tgttcgttct gcgctgacag aaggtctgga
    46321 agtaatgggc atttatgacg gctatctggg tctgtatgaa gaccgtatgg tacagctaga
    46381 ccgttacagc gtttctgaca tgatcaaccg tggtggtacg ttcctcggtt ctgcgcgttt
    46441 cccggaattc cgcgacgaga acatccgcgc cgtggctatc gaaaacctga aaaaacgtgg
    46501 gatcgacgcg ctggtggtta tcggcggtga cggttcctac atgggtgcaa tgcgtctgac
    46561 cgaaatgggc ttcccgtgca tcggcctgcc gggcactatc gacaacgaca tcaaaggcac
    46621 tgactacact atcggtttct tcactgcgct gagcaccgtt gtagaagcga tcgaccgtct
    46681 gcgtgacacc tcttcttctc accagcgtat ttccgtggtg gaagtgatgg gccgttattg
    46741 tggcgatctg acgttggctg cggccattgc cggtggctgt gaattcgttg tggttccgga
    46801 agttgaattc agccgtgaag acctggtaaa cgaaatcaaa gcgggtatcg cgaaaggtaa
    46861 aaaacacgcg atcgtggcga ttaccgaaca tatgtgtgat gttgacgaac tggcgcattt
    46921 catcgagaaa gaaaccggtc gtgaaacccg cgcaactgtg ctgggccaca tccagcgcgg
    46981 tggttctccg gtgccttacg accgtattct ggcttcccgt atgggcgctt acgctatcga
    47041 gctgctgctg gcaggttacg gtggtcgttg cgtaggtatc cagaacgaac agctggttca
    47101 ccacgacatc atcgacgcta tcgaaaacat gaagcgtccg ttcaaaggcg actggctgga
    47161 ctgcgcgaaa aaactgtatt aatgatttcg gaaaaaggca gattccttta gcctgaaacc
    47221 gatgacagaa gcaaaaaggc ctgatgcgct tcgcttatca ggcctacatg aattctgtaa
    47281 tctattgaat ttgcaaactt ttgtaggccg gataaggcgt ttgtgccgca tccggcatgg
    47341 acaaagcgca ctttgtcagc aatatgaggc ggatttcttc cgccttttta atcccccaac
    47401 atatactcgc aagttatagc caatcttttt ttattcttta atgtttggtc aaccttctgg
    47461 cacgctttgc tcatcacaac acaacataag agagtcgggc gatgaaaaag tggggcgtag
    47521 ggttaacatt tttgctggcg gcaaccagcg ttatggcaaa ggatattcag cttcttaacg
    47581 tttcatatga tccaacgcgc gaattgtacg aacagtacaa caaggcattc agcgcccact
    47641 ggaaacagca aactggcgat aacgtggtga ttcgtcagtc acacggtggt tcaggcaaac
    47701 aagcgacgtc ggtaatcaac ggtattgaag ctgatgttgt cacgctggct ctggcctatg
    47761 acgtggacgc aattgcggaa cgcgggcgga ttgataaaga gtggatcaaa cgtctgccgg
    47821 ataattccgc accgtacact tccaccattg ttttcctggt acgtaagggt aatccgaagc
    47881 agatccatga ctggaacgat ctgattaaac cgggtgtttc ggtgatcacg cctaatccga
    47941 aaagctctgg tggcgcgcgc tggaattacc tggcagcctg gggctacgcg ctgcatcaca
    48001 acaacaacga tcaggcaaaa gcacaggatt ttgttcgggc actgtataaa aacgtcgaag
    48061 ttctggattc tggcgcgcgc ggatccacta acacttttgt cgagcgcgga attggcgatg
    48121 tactgattgc ctgggaaaat gaagctctgc tggcagcgaa tgaactgggg aaagataaat
    48181 tcgaaatcgt cacgccgagt gagtctatcc tcgcagagcc aaccgtgtcg gtggtcgata
    48241 aagtggtcga gaaaaaagac accaaagagg tggcggaagc ctacctgaaa tatctctact
    48301 cgccagaagg tcaggaaatt gcagcgaaaa actactaccg tccgcgcgac gctgaggtag
    48361 cgaaaaagta cgaaaatgcg tttccaaaat tgaagttatt caccattgat gaagagttcg
    48421 gcggctggac gaaagcgcaa aaagagcatt ttgctaacgg cggtacgttc gatcagatca
    48481 gcaaacgctg atttccccag gataattatc aagcccggtg gtttctcgcc actgggtttt
    48541 ttatttgtca cgattttgcg ttacccttgc atctctttga ggtacaggga aaaaaagatg
    48601 aaaaaagcgg gtcttctttt tttggtgatg atagttatcg ccgttgtggc tgccggtatt
    48661 ggttactgga aattaaccgg tgaagagtca gacacgttac gtaagattgt tcttgagcaa
    48721 tgtttgccca accagcagga aaaccaaaac ccgtcgccat gtgcggaagt caaacccaac
    48781 gccggatacg tggttttaaa agatcgtcat ggcccactgc aatatctgtt gatgccaacg
    48841 taccgtatta acggtactga aagcccattg ttgaccgatc cgtcaacgcc taattttttc
    48901 tggttagcct ggcaggcgcg tgattttatg agccaaaaat acggccagcc tgttcccgat
    48961 cgcgcggtat ctctggcgat caactcccgc accgggcgta cgcaaaacca ttttcatatt
    49021 catatttctt gtattcgtcc tgatgtgcgc gaacagctgg ataacaatct ggcgaacatc
    49081 agtagccgct ggttgccact gccaggtggt ttgcgtgggc atgagtacct ggcgcgtcgg
    49141 gtaacggaaa gcgaactggt acaacgcagc ccatttatga tgctggcgga agaagtgcct
    49201 gaggcgcggg aacatatggg aagctacggg ctggcgatgg tacggcagag tgataactca
    49261 tttgtattgc tggcgacaca acgaaatcta ctgacgctga accgtgcttc agccgaagaa
    49321 atccaggatc atcagtgtga gattttgcgt taaggcgagg agtttaggaa agtaagtgcc
    49381 ggatatgaaa tccggcacct gtcagactta agcctgttta gccgcttctg cagctttaac
    49441 gatcacggcg aaagcgtcag ctttcagaga agcaccgcca accagcgcgc cgtcgatgtc
    49501 tggctgagca aacagttctg cagcgttaga cgcgtttaca gagccgccgt actgaatgat
    49561 cacttgttca gcgatgttag cgtcaacttt agcaatgtgg tcacggatga atttgtgaac
    49621 agcctgtgcc tgagccggag ttgcagattt gccagtaccg attgcccata caggttcgta
    49681 agcgataacc gcaccttcga atgccgcagc accctgagtt ttcagtaccg cgtcgatctg
    49741 acgtgcacaa acttcttcag ttttgcccgc ttcgttttcc gcttcggttt caccgatgca
    49801 cagaaccgga gtcagtccct gctctttcag caccgcgaat tttttcgcga tcagttcgtc
    49861 agattctttg tggtaagtac gacgttcaga gtggccgatg atgatgtact gtgcgccgat
    49921 gtctttcagc atagcagcag aggtttcacc ggtgaatgcg ccggacaggt tcaggtccac
    49981 gttttgcgca cccagcatga tgtggctgcc ttcagcttca cgcttcgcca tatcgatgta
    50041 catttccggt ggtgcgattg caaccgcaac cgcacagcca gcaacacctg ccagctcttt
    50101 acgcaggtta gataccagct cgtgaaccat gtggcggctg ccgttcagtt tccagttacc
    50161 catcactaaa ggatgtcgca ttttaattct ccacgcttat aagcgaataa aggaagatgg
    50221 ccgccccgca gggcagcagg tctgtgaaac agtatagaga ttcatcggca caaaggcttt
    50281 gctttttgtc atttattcaa accttcaagc gattcagata gcgccagctt aatcggttca
    50341 acagcgaagg tcagcccctt ttcgccgttg tccgcgacaa cataacgcag tgcaccttct
    50401 gtctcggtgt aataacgttt gtttttcccc gccgttagta gcgactgcag ttttttctgg
    50461 ctttgagttt tggtcattaa tggggtgagt gtgcggatca ccgctgccat gtatgcctga
    50521 gctttcgctt tcgcggcttt ttgctctggc ccctggatgg gtagccaggt tatttgaatg
    50581 cttttgattt ttaaggtacc gcgctccagc gcagtagaag catacaagtt ctcgttaatt
    50641 ttacttgctg cacgagtgag attggctttg tcgggactgc tgtcgatggc acgaaattcg
    50701 ttcagtggca ggctgggatt ctggctgtta aagtcttctc gaaactggct gatggagaga
    50761 tcgaaagtag gggctccagc cagcagataa ggcgcggtcg ttgccgtatc tggtgtttgt
    50821 gcatgcgccg ttgttgtaac ggttaatgca gaacataaga gaaaaaacag cgtacaccct
    50881 ggcttcattg acagtacctt ttgttatgac tggctccgat taaaacgata tctgtcgccc
    50941 ttgtcaaaag ggtagaatcc tggaaaagac aaccatcaag gaacttacat gaccatacag
    51001 caatggttat tctcatttaa agggcgtatt ggacgccgtg atttctggat ttggataggc
    51061 ctgtggttcg caggcatgct ggtgcttttc tcactggcgg gtaaaaattt actcgatatt
    51121 cagaccgcgg cgttttgcct tgtgtgcttg ctttggccaa cagcggcagt aaccgttaag
    51181 cgcttgcatg atcgcgggcg ttccggcgca tgggcatttc tgatgattgt ggcgtggatg
    51241 ctgctggcgg gtaactgggc aattttaccg ggtgtctggc aatgggcggt ggggcgtttt
    51301 gtcccgacat tgattctggt gatgatgctt atcgatcttg gtgcgtttgt tggcacacaa
    51361 ggcgaaaata aatatggtaa agatacccag gatgtgaagt ataaagccga caataaatca
    51421 agtaattaac ctttaatata tctcttatcg ctattttttt ccgggattgt tatttccacg
    51481 atgcaagtga caatgcccgg aaaatccttc tgaactcttc accttaagca atatcaaaaa
    51541 aaatggtaat ccataagatc attacttgtt tgttcttcct ttaacggcgc attattctta
    51601 agtcgtaatc gacagagagg cgaatataca gaggtgccct atgaaagatg tcgtagataa
    51661 atgcagtact aaaggatgtg cgattgatat cggtacggtg attgataacg acaactgtac
    51721 cagtaagttt tcgcgctttt ttgccacccg cgaagaagca gagtccttta tgaccaaact
    51781 gaaagagctt gccgccgctg catcctctgc agatgaaggg gccagcgtgg cctataagat
    51841 taaggatctg gaagggcaag ttgagcttga tgcggccttc accttctcat gccaggccga
    51901 gatgattatt tttgagttat cactgcgttc gttagcttga tggagttgct tcagaaggag
    51961 ttaaacatgg cttataaaca cattggcgtg gcaatttccg ggaatgaaga agatgcctta
    52021 ctggtgaata aagccctgga gctcgccaga cataatgacg ctcacctgac gttaattcat
    52081 attgatgatg gcttaagcga gttgtatccg ggtatctact tccctgcaac agaagatatt
    52141 cttcaattgt tgaagaataa gtcggataac aagctgtata aactgacgaa aaatattcaa
    52201 tggccgaaga caaaactgcg tattgaacgc ggagaaatgc cggaaacact gctggaaatt
    52261 atgcaaaaag agcagtgcga cctccttgtc tgtggtcatc accactcatt tatcaaccgt
    52321 ttgatgccgg catatcgcgg gatgatcaat aagttgagtg cggatctctt gatcgtgccg
    52381 tttatcgaca agtaaccgct taccagtaat gctccgctgt catatggccc ggtcggcgac
    52441 gtaaatgttt cgtcatctgc cgggtctcct tcagcaactg ttgtgtatcg cgcaccatct
    52501 gtggattgcc gcacagcatc acatggctgg tttctttatt catcggcagg ccaaccgcgc
    52561 tttccagttc accactttca attaacgccg gtaatcgccc agtgagcgaa cccgcagccg
    52621 tttcccgact gaccaccgtc tgaatgcgca gtttcccctc gtagcgtttt tccagttcct
    52681 gcatcagagg tagatagctt aagtcggcgg catatcgtgc ggcgtgaacc agcaccatgt
    52741 ttttgaagcg ttctaaatct ttgcccaatt gcagaatcga taaataaggg ccaatcgctg
    52801 taccggttgc cagcatccat agcgtttcgc aatctggtac ttcatccagc acaaagaagc
    52861 cagcggcttc gctaaccacc tgcacttcat cgcccggttt cagtgccgcc agtcgtggac
    52921 ttaatttgcc atcggggacg gtgaccaggt aaaactccag atccggatta tcgggcgagt
    52981 taacatagga gtaggcgcgc tgaacgcgtt cgccttcaat ttcaaggcca agtttggtga
    53041 attgcccggc ggtaaacgga agcacgggag cgtgaacggt gagactaaac agggcgtcgg
    53101 tccagttctg cactttagtg actttgcctg ttacccaatc agccatgttt ttctcctgtt
    53161 ttgattgact tgccttatct tcgttctccg aacaaaaaaa ttccagtccc gaaggactgg
    53221 aaggctcaat cgatcaaatc aatcagagga tgtgcacctg catttccggg tctttacgat
    53281 ccagatagtg gatggactga atgcgacgaa tggtgcgtga tttaccacgg atcagcagcg
    53341 tttcggtggt ggcgatattg cctttgcggc taataccttc cagcagatcg cctttggtaa
    53401 taccggtggc agagaagatg acgttgtcgc tgcgcgccat atcgcccaaa cgcaatactt
    53461 taccggcttc gatgcccatc gctttgcagc gtgccagctc ctgctcgcca atgcgacgat
    53521 tctcttcgtt gtcgcctttg acgtcatgac gcgccagcag acgaccgttc atgtcaccat
    53581 ctaatgcgcg gatcaccgcc gcagaaacca cgccttccgg cgcgccgcca atgccgtaca
    53641 gtacatcgac ttcactgtcc ggcatacagg tgagaattga ggccgcaacg tcgccatccg
    53701 gaatagcaaa tacgcgtacg ccgagttgtt gcatttctgc gataacggca tcgtggcgcg
    53761 gttttgccag aatcgttacc gtcagttcgc tcaacggttt gccgagcgcc gccgcaacat
    53821 tgcgcaggtt atccgccagc ggcaggttca gatcaatggt gcctttggct cccggtccga
    53881 cgatcagctt ctccatatac atatctggcg cgttgaggaa acagccttta tcgcctactg
    53941 ccagcaccgc cagcgcgtta gcctggccca tcgccgtcat gcgcgtgcct tcaatcggat
    54001 caacagcaat atctaccgcg tcgccgcgac cagtaccgac tttttcacca atgtagagca
    54061 tcggtgcttc gtcgatttca ccttcaccaa tgacgatggt gccgtcaatg ttgacctggt
    54121 tgagcataat acgcatggcg tttaccgccg cgccgtccgc ggtgttttta tcgccgcgtc
    54181 ctaaccattt gtagccagcc agcgctgctg attcggtgac gcgggaaaat tcgatggcaa
    54241 gttctcgtct catagcaaac tctaagcagt aaggaatggc gcgaagtgta gcacagggga
    54301 agggcgggat tattgatgtg tgcggggttg cccccgcacg gtttcagagt ctgtgtgatg
    54361 ccggatgtgt atccgactcg taggcctgat aagacgcgca agcgtcgcat caggcaatca
    54421 ttattactcg tcgtgttctt cccatgccat cgcgcgtttg accgcttttt tccagcctgc
    54481 gtaacggtaa ttacgctcag tggtttcgat gccaggacgg aactcgcgtt caatcaccgc
    54541 tttctcttgc agctcgtcaa gattctgcca gaagccaacc gccagaccag caagataggc
    54601 cgcacccaaa gcggtgactt cgcgcacttc cgggcgctca acgcgcgtac cgagaatatc
    54661 ggactggaac tgcatcagga aattgtttgc tactgcgccg ccatccacgc gcagggcgtg
    54721 cagtcgaata ccagagtcgg cctgcattgc ttccagcacg tcacgcgtct gataagcaat
    54781 agactccagc gtcgcgcgta taatgtggtt agcgttcacc ccacgagtca gaccgaaaat
    54841 cgccccgcgt gcatacggat cccagtatgg cgcacccagc ccggtaaagg ccggaaccac
    54901 atacacacca ttggtgtttt gcactttggt ggcgaaatat tcggaatcgt aggcgtcgtt
    54961 aatcaacttc atctcatcgc gcagccactg aatggacgcg cccgccataa acactgcacc
    55021 ttccagcgca tagttcactt cgccagtcgg gccgcaggcg atggtggtca gcaggccgtt
    55081 ttctgatttc accgctttct cgccagtgtt catcagcata aagcagccag tgccataggt
    55141 gttcttcgcc attccttctt tcacgcacaa ctgaccaaac agcgccgcct gctggtcacc
    55201 ggcgatcccg gagattggaa tacgcgtgcc gcctttgccg ccaatgttag tctgaccgta
    55261 tacttcggaa gaacgacgca cttctggcag catctcgcgt ggaatatcca gcacctccag
    55321 cattttgtcg tcccagtcca gagtatggat gttaaacaac atggtacgag aggcgttggt
    55381 gtaatcggtc acatggacac ggccctgagt cattttccag ataagccacg tatcaaccgt
    55441 accaaacagc aattcaccac gacgcgcacg ctcgcgagag ccttccacat ggtcgaggat
    55501 ccacttcact ttggtgccag agaagtacgg gtcaatcacc agaccggtgt tgttacgaat
    55561 gtactcttcc atgccatcgc gtttgagatg ttcgcagatc tcggcggtac gacggcactg
    55621 ccagacaatg gcgttataga taggtttgcc ggtttctttt tcccagacaa tagtggtttc
    55681 acgctggttc gtaataccga tagctgcaat ttgatcggaa ctgatatcgg ctttcgccag
    55741 cacttctacc agcgtggagc tttgggtggc ccagatttcc attgggtcgt gttctaccca
    55801 gcctggtttt gggtagattt gctcaaattc gcgctgcgac acgctaatga tattggcatc
    55861 gtgatccatt acgaccgcgc gggagctggt ggtgccctgg tcgagcgcaa cgatatattt
    55921 tttttcagtc atgtttaatt gtcccgtagt catattacag cgaagctttt tgttctgaag
    55981 gagttgtggt ctccttttct tccacaacac agatatcgca aggcaaatgg cgaccaatca
    56041 gtttgcgata ggcaaatgca cctacaatcg cgccaacgat agggccaaaa agcggcacca
    56101 ggaagtaagg aatgtctctg ccgccggtaa aggcgacatt gccccagccc gccagccagg
    56161 caaagacttt cggaccgaag tcacgcgctg ggttcatggc aaagcccgtc aatgggccca
    56221 tagatgcgcc aatgaccgca atcagtagac caatcagcaa gggagccaaa gggccgcgtg
    56281 gtacaccgtt gccatcgtcc gttaacgcca ggatcagccc catcagaata gcggtaatca
    56341 ccatctcaac tgcgaaagcc tgcacaaaat tgatatgagg attagggtaa gtagagaaag
    56401 tgccagccag atcaacactt tcaacgctgc cgcgaacaat gtgatgagtc tgctcgaagt
    56461 cgaaaaataa attgtagtaa agcccgtaaa ctaaagccgc agcacagaaa gcgccggcaa
    56521 cttgtgaaac gataaaagga ataactttgc gcttgtcgaa acaggcaaac agccacaatg
    56581 caatggtaac agcgggatta agatgcgcgc cggaaacccc tgcggtcagg tagatggcca
    56641 ttgccacccc cagtccccaa atgacactga tttcccactg accaaaagac gcaccagcga
    56701 cttttagtgc tgcaacgcaa cccacaccga aaaaaatcaa caacccggta ccgaggaatt
    56761 cagcaatgca ctggcctttc aaggttgatg tttgactcat aatcggatcc tgaagagtta
    56821 atgtttgttg tatgcgtgaa agtcacggac ctccacgatg cttgtaggca tgctgtaaac
    56881 ttatcgttaa cgagcaaaaa cgagaaatat cgaacttaaa atgtgtgtgc ttcgtcataa
    56941 aatgagcgtt atcgcgccat ttattccatc tgattgtaca tgatgcatgt ttgttagatc
    57001 attcgcgcaa caaatttatt aacattttcg attggtgtgg attatgtcac caggagaagg
    57061 atgaaaagtg tggcaaaccg taatctgcaa ggcgtgccgc tggacagcga tggcgcggct
    57121 tcatacaatc ggagctaact aaagtgcgct cgtatttatt aaggcgtcac cggtaatcgg
    57181 gacgaggatt tttatccatc aacgccttgc aattcaggag aggtatgaca atgtcattag
    57241 aagtgtttga gaaactggaa gcaaaagtac agcaggcgat tgataccatc actctgttgc
    57301 agatggaaat cgaagagctg aaagaaaaaa acaactcact gtcgcaggaa gttcaaaatg
    57361 cccagcatca gcgcgaagag ctggagcgtg agaacaacca tctgaaagaa cagcagaacg
    57421 gctggcagga acgtctgcag gccctgctgg gtcgcatgga agaggtctga ttacacttca
    57481 tctttcgcgt aatttcttta ctgtgtaggc caacaggtaa cgcagaaaaa aggcaccttg
    57541 cggtgccttt tttatcattc aatatccagc ggatcttctg aaagaataat cccggtattg
    57601 tcggcataaa gatggtcgcc ggagaagaag gtgacaccgc caaaattgac gcggacatcg
    57661 ctttcgccaa tgccttcgcc agcggcacca accggaattg ccgccatcgc ctggatgccg
    57721 atatccaact cttccaggtc atctacctga cgcaccgcgc cgtaaatgac cagaccttcc
    57781 cattcatttt gtactgccag acgcgccagt tcagcatcga ccagtgcgcg acgaacagaa
    57841 cctccgccat cgacgacaag aacacgacca cggccattct gttcgagcag atcgtacagc
    57901 aacccgttgt cctcgaaaca ttttaccgtg attatttgtc cgccaaacga cgcccgtccg
    57961 ccaaagttgg agaacagcgg ttccacgacg ttaacatctt cttgatagat gtcacaaagc
    58021 tcggaagtat cgtatttcat aggcttaaca ttcagttgct gcgagaattt tcagtatatc
    58081 gcgctatgtg ggctgttggc aaaatcatca attgttaatt gatatttgtc agttatgctg
    58141 cccactggct taggaatatc cctaaaacaa acagcaggtt agtcagtaac gctcctttga
    58201 cagtgcgttc cagcattggt cgcatcgcca ccgggtccat ttcgcgcatc acataacgag
    58261 cttgcttcac cagcaacggt gccgccagca ggaacagcca gccccacagg ctatgcagcg
    58321 aaaagagatt aaacagcgcc agacacacca gtgagcccat cagcaggcag gcatgataac
    58381 gacgcgcgtt tactgcgcct aagcgcacca ccagcgtgtt tttgccgttt tcgcggtcgc
    58441 tattgatatc acgcaggtta ttaatgttca gtactgccgt tgccagcagg ccgcatgcgg
    58501 ttgccggaag gatcagtgcc ggaatcaatg tatgagcctg taaataccag ctccccatga
    58561 cgctcaacca gccaaagaaa accagtacgg aaatatcacc caaaccgata taaccataag
    58621 gacgattgcc cacggtgtag gtgatagcgg caatgatcga caacccgcca agaatcagga
    58681 aaccgacaaa atcggccagc gtatggcacg ccactgcaac cagtgccagc ccggaaagac
    58741 agatgagcac aacggtaata atgagcgccc gtttcatctc ttgctgggta atgacccctt
    58801 tttgcatgcc gcgtagcggc ccaatgcggt caggtttatc gctgcctttt accgcatcgc
    58861 cgtaatcatt ggcgaggtta gaaaggatct gtaatagccc ggcggtaatc aatgccagca
    58921 gggcgaccag tggatcgaag tgaccttgcc accatgccag cgccgtcccg acgataattg
    58981 cagcaaaggc gagggggagg gttttaggtc gtaaactttc cagccacgcc tgagttcggc
    59041 taatttgttg ttcagtcata atacgcgcca ataaaaatgg ggcctttcag ccccatcaaa
    59101 caatgatgaa aatgattgaa cgcgattata ggataaaacg gctcagatct tcatctgcca
    59161 ccaacgcatc cagatgtttg ctcacataat ctgcgtcaat agtgatagtt tgaccgctta
    59221 aatcgctggc gtcgtaggaa atctcttcca ttaaacgctc cagaacggta tgtaaacgac
    59281 gagcaccgat gttttcggta gattcgttca cctgccatgc cgcttccgcg atgcgcttaa
    59341 taccggagtc ggtaaactcg atatttacgc cttcagttgc catcagtgct ttgtactgca
    59401 cagtgataga ggcattcggc tcggtcagaa tacgctcgaa gtcgctggtg gtcagtgcct
    59461 gcagctcaac gcggattggc agacgacctt gcagttccgg gatcaggtca gatggtttcg
    59521 caatctggaa cgcgccagaa gcgataaaca gaatgtggtc agttttgacc atcccgtgtt
    59581 tggtggaaac agtgcaacct tctaccagcg gcagcaggtc acgctgaacg ccttcacgag
    59641 aaacatccgg accggaagac tcgccgcgct tacagatttt gtcgatttcg tcgataaaca
    59701 cgatcccgtg ctgctcaaca gcgtcgatag catcttgctt cagctcttcc gggttcacca
    59761 gtttcgccgc ttcttcttca atcagcagct tcatggcgtc tttgattttc agcttacgcg
    59821 ctttttgctt ctgaccgccc aggttctgga acatggactg cagctggctg gtcatctctt
    59881 ccatgcccgg aggagccata atttcaacgc ccatcggtgc tgcggcaaga tcgatctcga
    59941 tttctttgtc atcaagctgg ccttcacgca gttttttgcg gaatgcctga cgagcagcgg
    60001 acggttcctg ctgctgttcg gtctgtcccc agttgttttt agccggtggg atcagtacgt
    60061 cgagaatacg ttcttctgcc agttcttcag cgcgataacg gtttttctcg atagcctgga
    60121 cgcgtaccat tttcacggcg gcatcggtca gatcgcgaat aatagaatcc acttccttac
    60181 cgacatagcc cacttcggtg aatttggtcg cttcaacttt gatgaacggc gcattcgcca
    60241 gcttagccag acgacgggcg atttcagttt taccgacacc ggtcgggccg atcatcagga
    60301 tatttttcgg ggtcacttca tggcgcagct cttcgttgag ctgcatgcga cgccagcggt
    60361 tacgcagagc aatcgccacg gaacgcttgg cgttgtcctg gccgatgatg tgcttatcca
    60421 gttcgctgac gatttcgcgt ggggtcattt cagacatggg agatccttac gctttgtagc
    60481 ttaattcttc gatggtgtgg aaatggttgg tatagatgca aatgtcgcct gcaatatcca
    60541 acgccttttc agcaatttca cgggcgctaa gttcagtgtt ttctaacagc gcgcgcgccg
    60601 cagcctgggc gtaagggccg ccggagccga tagcaataag atcgttttct ggctgcacca
    60661 cgtcaccgtt accggtgatg ataagcgatg cagtttcatc cgcgactgcc agcagtgctt
    60721 caagtttgcg cagcatgcga tcggtacgcc agtctttcgc cagctcaacg gcggctttga
    60781 ccagatggcc ctgatgcatt tccagtttac gttcaaacag ttcgaacagc gtaaaagcat
    60841 ccgcagtacc gcccgcaaag cccgcgatga ctttgtcgtt gtacagacgg cggacctttt
    60901 tcacgttgcc tttcattacg gtattgccca acgtggcctg accatcacca gcgatgacca
    60961 catggccgtt acggcgtacg cttactatag ttgtcacgag ctgacccctt ggttacgaat
    61021 acagagtaca aaccccgtac aaaagtacgg ggcataatgc aattatagat gggggggatt
    61081 ttgagggttt caacccccgg cggcgagccg aatgcagttt gtatgacccg ccatcttcaa
    61141 gcgattgagg gtactgtctg cgttctcttt gcctttcacc gggccaatca ccacacgatt
    61201 ccagccattg ttggtggtaa tttttgagtc aaagccttcg aacgccagct gagcacgtac
    61261 cgtctctgcc tgttccgcgc ctctgaacga accgcactgc accatccagc ggcgttcgtc
    61321 ttttttctcc gccgtcggtt ttggcgcgtc agcggcacga gcaaccggcg cagcttgctg
    61381 cggcttcgac tgcgcggtcg tgtgcgcagg ggtttgcagc agatcctggt acggctgctg
    61441 agtataagcc ggtttagact ggcgcggttg cgcctgtacc ggcgcggctt gcgacgtacg
    61501 cgtctgctgc tgccagcttt gttcagtcgt acgcgactgc tgaaccaggc gttgctgctc
    61561 cgctaattgc tgcgcctgac gctggcgctg tagcgtttgc tgacgctgtt ccggcgtctg
    61621 ttcattccac ggaacttcaa ccagctgcgt tggctgctgg cgcatatcag cctgcatttg
    61681 ctcaagaagc tggcgttgtt ctggtgtcag ttgctccggc gttttcactt caccaccggc
    61741 agaaggttct gtaggcgcac gcacacccgg ctggcgactt tccagctctt tgatgtagcg
    61801 ccagcgttct tctggttttg gtggtagtcc gtttccggtc actttctggc tttgcagcgt
    61861 ctcggactct tctttcttgt gatgcgtaat gaagtacaga ccaccgataa aggtcacaag
    61921 aacggcggca gcaatagcga ccatagcggg agaaaccgca ggcagatttc gttgcttttt
    61981 ccgtgaggtg ctcttttttc gccgcgaagg tgccggttgg ctgcggcgta cataatctcg
    62041 ttgtgccact atcgtttcgc tgtatttatt cgttcgtcag cccgccatgt tacttaagcg
    62101 gcgggccttt gaccagtcac ggcagtctta aaggtttact ttaaggtaac gcgcgtgttg
    62161 atccccggat gataagttcg cagtccatta aacgagagcc actgccaacg tgttgcccct
    62221 gcatttgatc aagcaataac agcatagctt cccgaccgat ttcgtaacgc ggctgcgcga
    62281 tggttgtcag cggcggatca caaaattgcg tcaggtcgat gttatcaaaa ccgattatgg
    62341 aaaggtcttc cgggactttc agcccctggc gttttgcctg agaaagtgcg ccgagcgcca
    62401 tcacatcgct atggcagaag acagcagtag gcggttgtgg aagatcaagc agctgctgca
    62461 ttgctttgct tccagcttcg aaggtgaagt cgccacgggc gatgtattgc ggatcaacca
    62521 taatgccgca gcgacgcagc gcctggacgt agccttgcag gcggtagtga cacagcggca
    62581 tctcttcggg accggctata cagccaatcc gattatgccc ttgctcatat aaataattta
    62641 ctgcatcaaa tgcggcggtc agattgtcga tatgaacggt aggcagctcc agttccggtg
    62701 caaattcgtt cgccatcacc atcggcggca gattacgctg ttcctcaatg ctggcatcaa
    62761 acggcagcct tgaacccagc aacaacatgc catcaatttg cttggtgatg atcaaatcga
    62821 taaaggtttt ttcctgctga ttttgatgcg cacagtcgcc aatcagcacc agatatccgt
    62881 gatttgccgc cgtaacttcg ataccgcgaa taatttcgct aaagaaggga tcgcagatat
    62941 ccgggacaat caccagaatg gtacgggatt cattacgctt gacgttgcgc cccataggct
    63001 gtggtaaata acccacttcc cgggccgctt tttcaacccg attacgagtg gcctgggaga
    63061 ctttatcggg attcattaat gctcgggaga cggtcgctgt agagactttt gccttgaggg
    63121 caacgtcttt catggtcgcg gcagtttcct gcttcttcgc tttcacactc actcctcgcc
    63181 tggcacggca ggcgtactac ttccatgttt acttcacatc ggcaacattt ttagcagata
    63241 gcgcgtgaaa acggttacag aattttcatg aaaagtgtga tgaatattga atttttcgat
    63301 ccgcctcgca tcgtttgcgc attagccctc gatgggatcg acatccagca cccatttcac
    63361 cttacgggaa tccggtattg tattaataag cgccagcgta ccattgatga tgtgctgtaa
    63421 tcgcacgcgg gaagggtgct gcaacaaaat ttgccagcgc cagcggccac cacgcttagg
    63481 ggccagtgcc ggaaccggac cgagaaccca cagtttgtcg tctgcaagcg ggctggcgag
    63541 gatcagatta cgcaactgtt gcaggaacag cggcgcgtgc tgattgttat gatcttccgc
    63601 acgcacaatc acatggttgg tccacggcgg cagttgcatc atgcgtcgtt cagccagtgc
    63661 ctgttcggca aaggcgtcgt agcctttata gagcaacgtt tgcaacagcg gatgttcagg
    63721 atggtgcgtt tgcagaacta cttcaccctg tttacccgca cgcccggcac ggcctgccac
    63781 ctgggtataa agctgggcaa aacgctctgc cgagcgaaaa tccgcagaaa acagcgcgcc
    63841 gtccacatcc agtaacgcca ccagcgtcac atccggaaag tggtgacctt tcgccagcat
    63901 ttgcgtgcca atcaaaatcc gcgcgccacc gcgatgtact tccgccagtt gttgttccag
    63961 cgcgcctttg cggctggtgg tatcgcggtc gatacgagaa atggggacac cagggaacaa
    64021 cggcgcgagc gtctgttcaa gctgttcggt gcccagcccc acgggtacca ggtgcgtgga
    64081 accgcaagaa gggcactggc gcggcaccgg acgctgactg tcgcaatggt ggcagcgcaa
    64141 atgctgttgc gcctgatgca gcgtgtagta gtgatcgcaa cgtgggcatt cggctatcca
    64201 accacagtcg tggcacagta gcgcaggcgc aaaaccccgg cggttaagaa agaggataac
    64261 ctggttgtcg gcctgtaaat gctggcgcat acgggtgatt aacgccggag ccagacctgc
    64321 ctgcaccttc tgacctttta aatccagcac atgttgaatt gccgggcgcg cattgcccgc
    64381 ccgacgggtc aggcgcagca ggcggtattt tttctgctgc acgttgcata acgtttccag
    64441 tgcgggcgtt gcagagccaa gaataattgg gatttgctcg ctatgcgcac gatacaccgc
    64501 caggtcgcgg gcatgatagc gccagccttc ctgctgcttg taggagctgt cgtgctcttc
    64561 atcaataaca atcacgccga gatttttaaa cggcgtaaac agcgcggagc gggtgccgat
    64621 gacaatcgcc gcctcgccat ttttcgcttt caaccacgcc gaaagacgct cgctgtcgtt
    64681 caggccggaa tgcagaactt ccaccggggc attaaaacgt tcacgaaaac gggcgatagt
    64741 ttgcggtgtc aggccgattt ccggcaccat caccagcgcc tgtttgccct gagcgagcac
    64801 gttttccagt acactgaggt aaacctccgt tttaccggaa ccggtaacgc ccgccagcag
    64861 ccaggcagaa aaagtatctg ccgcgctatg aattgcgcca acggcggtgg cctgttcggt
    64921 attcaaccgc aaccgctcac cagaaacggc atagttcgtt cgccagtcgc taaactctgg
    64981 tgtttcactt gctaaatcac acagaccttt tttgcgcagt gcctgcaacg cggcatcatt
    65041 aaattcgagc gtggcaacct ggtcgcgcca gattttgcct tgccgtaacg ctgccagcgc
    65101 ctgttgttgc tttggcgaac gtttcaggct gttcagatcc accgcctgac cttgttcagt
    65161 ggcaaaccag taccacatcg gcgcgttcgc cgcaggccgc ccctggcgca gtaaaatcgg
    65221 caaggcatga aacagcacat cgccaatcgg atgatgatag taatctgccg cccagagcag
    65281 taatcgccag atggagtgag taaataccgg ctcaacgtcc agcacttcaa ctaccgcttt
    65341 cagctcgtta agcggcagct cactgacatc gctcactgac accacaaccc cgatgcgctc
    65401 ctgttgtttg ccaaacggca cgcgcacgcg actcccggct ttaaccgtca tgccttctgg
    65461 cagcagatag tcaaaggtac gaggaagcgg aacgggcaag gcaacgtggg caacgggcat
    65521 agcatcatcc tgacttgaaa ttcggtgggt tagtatacac attgccgtag aagagtgcgg
    65581 atcagtttgc atacgctggt taatttctgt atgatttcgc gccttcgtac gaaatgatcg
    65641 tattgaagct atacttttaa catcgcgtgg tgtctggcgt tagggctgga agagcgacgc
    65701 ggccttaaac cgaggttttc ccatgaaaaa agatattcac ccgaaatacg aagaaattac
    65761 tgctagctgc tcttgcggta acgtaatgaa aatccgctcc accgttggtc atgacctgaa
    65821 cctcgacgtg tgcagcaagt gccacccgtt cttcactggc aaacagcgtg atgttgctac
    65881 cggtggccgt gttgaccgct tcaacaagcg tttcaacatc ccgggcagca aataagaccg
    65941 gatttccgaa aaaagcgccg cacggcgctt tttttgtgcc tgaattttgg tcgtattacc
    66001 aggaaaacag cggccattct ttggcgaccg tggtgagttg tggcgcgtcg aaaacggctt
    66061 gtggtgaaac taccgtttcc tcaagcggaa tatttttgcc gtagcgttct ttgatctttg
    66121 cctgaactag cgggttacta agatcgaact ctttcagctt ctgctgctcg cccacacgca
    66181 ttcccagcgc attctggtag accttccaca ccacttccga acagtactgg cgatcgtcgc
    66241 tccacgagaa gctggagtca tacggtttac cgagataacg ttttgccgtt tgcgccagtt
    66301 tttgctgttg ttcaacactc agtccgcctt caacgcggcg aacaacgtat ttgccctttt
    66361 caccatgggc gatccactgc ttgagtgggg tgtattttac cgggccggcc gcttcaaaaa
    66421 cgtagggctt tttgttgcgt atcaccagca taccggtgtg gctgtaatcg gaatgggtcg
    66481 ccagttggat cgctttactt tgcgatgagc gagagatctg aaagatgatg tcgccggttt
    66541 gtggttgcca ggcaaatgca ggcacagaaa ccagcaggct gagaatcagt agcctgattt
    66601 tcatcgttaa acgtccatgt atgaaaaaag cggctggtta actccgcgcc gctcttttcc
    66661 gctttagtat tcccacgtct ccgggttaat ccccatctca cgcatgatct cttttgccgc
    66721 ctccggaatt tcgtcgctgc gctctttacg caggtcggca tcatccggca aaggttgccc
    66781 ggtaaaggca tgcagaaacg cttcgcacag cagctcgctg ttggtcgcgt gacgcaggtt
    66841 gttcacctga cgacgcgtgc gttcatcggt gaggattttt aacaccttaa gaggaatgga
    66901 aaccgtaatt tttttgactt gttcactctt cttgccgtgc tcagcgtatg ggctgatata
    66961 ttcgccgctc cattcagcca tgagatactt aatcctcttc gtcaataaat tgaggccaga
    67021 ccacacaatt aatatgggta ctgaccgtaa acccgcatag tttaccgtac aggcgttacc
    67081 gtgacatcgt gtaatgcacc tgtcggcgtg ataatgcata taattttaac ggctatttga
    67141 gatttgctca atctatacgc aaagaagttt agatgtccag atgtattgac gtccattaac
    67201 acaatgttta ctctggtgcc tgacatttca ccgaccaagc ccagggaact tcatcacatg
    67261 acgcgtaaac aggccaccat cgcagtgcgt agcgggttaa atgacgacga acagtatggt
    67321 tgcgttgtcc ctcccatcca tctttccagt acctataact ttaccggatt taatgaaccg
    67381 cgcgcgcatg attactcgcg gcgcggcaac ccaacgcgcg atgtggttca gcgagcgctg
    67441 gcagaactgg aaggcggtgc tggtgcagta cttactaata ccggcatgtc cgcgattcac
    67501 ctggtaacga ccgtcttttt gaaacctggc gatctgctgg ttgcgccgca cgactgctac
    67561 ggcggtagct atcgcctgtt cgacagcctg gcgaaacgcg gttgctatcg cgtgttgttt
    67621 gttgatcaaa gcgatgaaca ggcattacgg gcagcgctgg cagaaaaccc caaactggta
    67681 ctggtagaaa gcccaagtaa tccattgtta cgcgtcgtgg atattgcgaa aatctgccat
    67741 ctggcaaggg aagtcggggc ggtgagcgtg gtggataaca ccttcttaag cccggcatta
    67801 caaaatccac tggcattagg tgccgatctg gtgttgcatt catgcacgaa atatctgaac
    67861 ggtcactctg acgtggtggc aggcgtagtg attgctaaag acccggacgt tgtcactgaa
    67921 cttgcctggt gggcaaacaa tattggcgtg acgggcggcg cgtttgacag ctatctgctg
    67981 ctgcgcgggt tgcgaacgct ggtgccgcga atggagctgg cccagcgcaa cgcgcaggcg
    68041 attgtgaaat atctgcaaac ccagccgttg gtgaaaaaac tgtatcaccc gtcgctgccg
    68101 gaaaatcagg ggcatgaaat tgccgcgcgc cagcaaaaag gctttggcgc aatgttgagt
    68161 tttgaactgg atggcgatga gcacacgctg cgtcgtttcc tgggcgggct gtcgttgttt
    68221 acgctggcgg aatcattagg gggagtggaa agtttaatct ctcacgccgc aaccatgaca
    68281 catgcaggca tggcgccaga agcgcgtgct gccgccggga tctccgagac gctgctgcgt
    68341 atctccaccg gtattgaaga tggcgaagat ttaattgccg acctggaaaa tggcttccgg
    68401 gctgcaaaca aggggtaaaa atgagtgtga ttgcgcaggc aggggcgaaa ggtcgtcaac
    68461 tgcataaatt tggtggcagt agtctggctg atgtgaagtg ttatttgcgt gtcgcgggca
    68521 ttatggcgga gtactctcag cctgacgata tgatggtggt ttccgccgcc ggtagcacca
    68581 ctaaccagtt gattaactgg ttgaaactaa gccagaccga tcgtctctct gcgcatcagg
    68641 ttcaacaaac attacgtcgt tatcagtgcg atctgattag cggcctgcta cccgccgaag
    68701 aagccgataa cctcattagc gcttttgtca gcgatcttga gcgcctggcg gcgttgctcg
    68761 acagcggtat taacgatgcg gtgtatgcgg aagtggtggg ccacggtgaa gtgtggtcgg
    68821 cgcgcctgat gtctgcggtg cttaatcaac aagggctgcc agcggcctgg cttgatgccc
    68881 gcgagttttt acgcgctgaa cgcgctgcgc aaccgcaggt tgatgaagga ctttcttacc
    68941 cgttgctgca acagctactg gtacaacatc cgggcaaacg tctggtggtg accgggttta
    69001 tcagccgtaa caacgccggt gaaacggtgc tgctggggcg taacggttcc gactattccg
    69061 cgacacaaat cggtgcgctg gcgggtgttt ctcgcgtaac catctggagc gacgtcgccg
    69121 gggtatatag cgccgacccg cgtaaagtga aagatgcctg cctgctgccg ttgctgcgtc
    69181 tggatgaagc cagcgaactg gcgcgtctgg cggctcccgt tcttcatgcc cgtactttac
    69241 agcccgtttc tggcagcgaa atcgacttgc aactgcgctg tagctacacg ccggatcaag
    69301 gttccacgcg cattgaacgc gtgctggcct ccggtactgg tgcacgtatt gtcaccagcc
    69361 atgatgatgt ctgcttgatt gagtttcagg tgcccgccag ccaggatttc aaactggcgc
    69421 ataaagagat cgaccagatc ctgaaacgtg ctcaggtgcg cccactggcg gttggcgtgc
    69481 ataacgatcg ccagttgctg caattttgct acacctcaga agtggccgac agtgcgctga
    69541 aaatcctcga cgaagcggga ttacctggcg aactgcgcct gcgtcagggg ctggcgctgg
    69601 tggcgatggt tggggcgggc gtcacccgta acccgctgca ttgccaccgc ttctggcagc
    69661 aactgaaagg ccagccggtc gagtttacct ggcagtccga tgatggcatc agcctggtgg
    69721 cagtactgcg caccggcccg accgaaagcc tgattcaggg gctgcatcag tccgtcttcc
    69781 gcgcggaaaa acgcatcggc ctggtgttgt tcggcaaggg caatatcggt tcccgctggc
    69841 tggaactgtt cgcccgtgag cagagtacgc tttcggcacg taccggcttt gaatttgtgc
    69901 tggcaggcgt ggtggacagc cgccgtagcc tgttgagcta tgacggtctg gacgccagcc
    69961 gcgcgttagc cttcttcaac gatgaagcgg tcgagcagga tgaagagtcg ctgttcctgt
    70021 ggatgcgcgc ccatccgtat gatgatttag tggtgctgga cgttaccgcc agccagcagc
    70081 tcgctgacca gtatcttgat ttcgccagcc acggtttcca cgtcatcagt gccaacaaac
    70141 tggcaggagc gagcgacagc aataaatatc gccagatcca cgacgccttc gaaaaaaccg
    70201 ggcgtcactg gctgtacaac gctaccgtcg gcgcaggctt gccgatcaac cataccgtgc
    70261 gcgatctgat cgacagcggc gataccattt tgtcgatcag tgggatcttc tccggcacgc
    70321 tctcctggct gttcctgcaa ttcgacggta gcgtgccgtt taccgaactg gtggatcagg
    70381 cgtggcagca gggcttaacc gaacctgacc cgcgtgatga cctttccggc aaagacgtga
    70441 tgcgcaagct ggtgattctg gcgcgtgaag cgggttacaa cattgaaccg gaccaggtac
    70501 gtgttgaatc actggtgcct gcccattgcg aaggcggcag catcgaccat ttctttgaaa
    70561 atggcgatga acttaacgag cagatggtgc aacgactgga agcggcccgc gaaatggggc
    70621 tggtgctgcg ctacgtggcg cgtttcgatg ccaacggcaa agcgcgtgtg ggcgtggaag
    70681 cggtgcgtga agaccatccg ctggcatcac tactgccatg cgataacgtc tttgccatcg
    70741 aaagccgctg gtatcgcgac aacccgctgg tgatccgcgg gccaggtgca gggcgcgacg
    70801 tcaccgccgg ggcgattcag tcggatatta accgactggc gcagttgttg tagtgattat
    70861 tgaacaaaag ctggcagttg ccagcttttg ttgtcacccc taaacctctt cctggggaag
    70921 tggtgattga ttgtcatttt gcctttggtc agtgcccccg gtaataaggg ggcttttgtg
    70981 ttgagcgatg aagtaacaga gtgaatggtg aagttattaa tgacaattac aaaatgacat
    71041 taagtaacaa tatctaaaat gtcacctaat ttaaaattga taaccaatag tagtataggt
    71101 tccccatcct gtatttttta attcaaatgg accatctccg aagttaacct tctctcccgc
    71161 attccattgt ccaccgttgt gaaaataacg tagtgtaaat gcaaatttcc agtgttcata
    71221 tagaagggaa aggatatggc ttgaagcaat agcattactg gttcgtttat tattgtgtga
    71281 cttgtccgca agatcggagc caaaatcaaa gttagtaaaa ctattgtata ccaatcgccc
    71341 tccgaataaa tttgtaagag ggatgctata ttttattttg aatcgatatc cgtcccattc
    71401 attttcatta gcggcaccat agttttgcca ctgatacttg gcgtatatat tggcagaaag
    71461 cttaattggt agacccgtgt cgatatctgt accaagcccc atataccatg tactttgccg
    71521 ggatgactgg ttgtcaccca tatcatagac atagttgttt gcaatgaacc attctttaaa
    71581 tgggccgaac gcaagattta atccggtcaa tttgtcaatg gaaaaccgag gttctatttc
    71641 cgtaaatagt ggtgagccct catcccagat cccaacatca tagtgactgc cgacgccaaa
    71701 gaatttcggt agatcaacat aaccataaaa atcaaaccag tcttttcttc ctgctacagt
    71761 atattcaggg tagaggtcgt tagtcagtct tgggccaaag cgagagtctg tgcgaccaat
    71821 gacattcaag ctttgatgta accagctttc atcaatttta atttcctgag cgtttccaat
    71881 gcaaggtatt aatagaatgg ataaagctat ccagtgcttt tttatataca taaatatatc
    71941 tcttatatat tgttgattta gagagaaata atgtcatcca gtgaatgaat ggtattataa
    72001 gtttttgtta tttgcttact tctacccgca tttcatttac ctgtgcagga gtaatcttgt
    72061 ggcctttctt aaaataatcg attacgacat tggacatgtt atttccggcg cggacctgga
    72121 catttttccc ttctttgaat gccagaaaac catcgccccc aagtgcaagg aaagtcgtag
    72181 tcgcaacatg gtaaatattc gtatcaacga tctcttcacc attaatttta aaagatacaa
    72241 tgcgctgacc tactggtttc tgcagggtat agcgcatttc tgcgcctttt gacatttgca
    72301 ggatgccatt ggtaagcgat gccccgtgtt ccagtaggtt acgcagatcc ttaccgctga
    72361 gatccatttc agtaagttca ttcttgaagg ggaatgtact ggtaatgtca ccaaaggtaa
    72421 tcgtgcctgc atttatatca gcacgtaaac tgccggagtt tatcaacgca atttgtgcag
    72481 tcggtgcagc gacaagcatt gcatcggtaa acaggttgcc aagttgcgat gactcaccgt
    72541 aggcacgagt taatgcaatc gatgattcac cgactggttg gcgtacaaga tccgccagtt
    72601 ttttattcca gccattgata actttttgtg tggttggatc aggtatccat tcatcggcat
    72661 aaaccgtttt cagctcaaag ctcttcactt tatgagtacg agctgtaggg ttgacatcaa
    72721 ggattaattt accaatatcg ataccgccac tgtcagtcga aaggattaag gtgttgccga
    72781 ctttgattgg ttctggcgtt ccgacgtggg catgcccggt aatcagaata tccaatcctt
    72841 tcaccttgct tgcggtttga atatctttat ccagcgcacg tctaacatct gtattgccaa
    72901 tgctggactg acgcgccggt acgccttcat gaaccagcgc cacggtgagg tcaaccttat
    72961 cacgtaactc gtcgatataa tgctgcaaat acttcacttc atcacgtgct tcaatcccct
    73021 gattcttgag gggcgcggaa gatttatcaa atcggttatt attgtcatta tcgagtccct
    73081 ggagagagag ctccgagacc gtgtcattaa atgcaaatac gccgtgtagc ccaacaatac
    73141 caatcttgat gccatctttt tccaggatgg tgtagggctt gttccagaag ggtttctcac
    73201 tctctttatg ataaacattg cctaataaaa cagggaaatt cgctttgctt aattgtcgga
    73261 gggcattatc ccagccatgg tcaaactcat gattaccgat tgatactgcg tcaaaaggca
    73321 tggtattcat aatatcaatt attgcttcgc cctttgtcag gctactgata tatggcccgg
    73381 tgaaatagtc tccagcatca aaataaaagg tggctttatt tttggctttc tcttgtttta
    73441 ctaacgtgga tatattggca aagccaccaa tgtcgcgttt tccgtctgcg acatacggta
    73501 ctttatagga atccacatga gcgtgaagat cattggtata aataatcgtg acatcctgcg
    73561 ccataactga ggtcgaaaaa atagtcagca atgttgcggc aaataaagta cgctgcgctg
    73621 ttttcattcg cattccttgt taaataaatt gactgataaa tgagatttta tttggcgacg
    73681 tccatgactc gtttagttaa gtctggggtg acaggtttat gatttttgaa ataatcgatg
    73741 atagattccg ccgaggttgt tcccggtatc gtcttcacat tttttccatt caaaaatgct
    73801 tcaaaaccgt caccaccact ggcacaaaag gagtgcgtgg caatacgata tgtttgatta
    73861 tcttccacgg gtttgccgtt aatggtgaaa gagattaacc gttggttgaa tggttttttg
    73921 ctgtcataga caaccgcaac acttttagaa acttgtaaca caccgttagt tagattggtt
    73981 gcatgcgtca tcaatgattt gagatcgcgg cccgtcaaat ccatctcgat gaggtcatta
    74041 ttaaagggga atgtgctaat aacatctccc agtgtgatat cgcctttagg aaaatcagct
    74101 cgcatcccac cgctattttg aaatccggca acggcatcag gtgttttttc catcattgca
    74161 tcaagaataa ggttgccggt agaagaggaa ctaccatact cacgtgttaa aactacggga
    74221 gaatggccga caacttcctg tgttattttg ttaagctttg cactccattt atcaatagta
    74281 ttttgaacga tggtgtcagg tttaaattga tctgcaaaga tggtgattaa ctcaccatta
    74341 taactatcaa ttttcttcgt tttcggattg taatcaagca caagttttcc gatgttggtg
    74401 ccgtatgcat cggtagatac aattaatgtg ttattaactt ttattggttg cggtgttccg
    74461 acatgtgcgt gaccggtaat taagacatca acaccattca cttttttagc tgtctcaata
    74521 tctgcctgta gtagccgctc cacatcttta ctaccaaagc ttgattgacg cgccgggaca
    74581 ccttcgtgaa ttagcaaaac ggtaatgtcg actttacctt ttaattctgc aagtgcttta
    74641 tttaaatatt taatttcgtc tctggcttct acgccttcac gtgcttttgc cgcgactgta
    74701 tcatagaaag cgaatgcgcc atgcaatcca atcacaccaa tctttaaacc atctttttcg
    74761 ataatggtcc acggatgatc ccaaactggt ttattcgtat cggtgtagta gatatttccc
    74821 agcagaatgg ggaattttgc ttttgataac tgagacacca tattgggcac accgtgatcg
    74881 aactcgtgat taccaacaga caccgcatca aagggcatag tgttcatgat atcaataata
    74941 gcttcacctt tggtcagtgt gctgatatac ggcccggtaa aatagtcgcc tgcatcaaag
    75001 aaaaacacat ctttgctttt tttcttcgct tcattaacga tgcctgcaat attagccata
    75061 ccacctataa gtctgttctt atcaacagcg atgatttttg ccggattgac atgtgcgtga
    75121 atgtcattcg tataataaat agtaacgtcc tgagctagaa cactgtgaga aaatatcacg
    75181 ccgaatatga ctgataatgt tgtaaataca gttttcatca tccctcctgg atagggtgtg
    75241 ttacatttgt tgaattattt aaataaaagg cacatcgtgt cttattttat gtaagcatag
    75301 ttcagaaa
//
LOCUS       DAZFRE010000012        74783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFRE010000012 DAZFRE010000000
VERSION     DAZFRE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.012"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..404
                     /locus_tag="ACHKUI_000955"
                     /pseudo
     CDS             1..404
                     /locus_tag="ACHKUI_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(689..1306)
                     /gene="tdk"
                     /locus_tag="ACHKUI_000956"
     CDS             complement(689..1306)
                     /gene="tdk"
                     /locus_tag="ACHKUI_000956"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6597211.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            1910..2323
                     /gene="hns"
                     /locus_tag="ACHKUI_000957"
     CDS             1910..2323
                     /gene="hns"
                     /locus_tag="ACHKUI_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6597212.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2467..3375)
                     /gene="galU"
                     /locus_tag="ACHKUI_000958"
     CDS             complement(2467..3375)
                     /gene="galU"
                     /locus_tag="ACHKUI_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6597213.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEDEMGIKK"
     gene            complement(3577..4590)
                     /gene="rssB"
                     /locus_tag="ACHKUI_000959"
     CDS             complement(3577..4590)
                     /gene="rssB"
                     /locus_tag="ACHKUI_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6597214.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRVLFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4682..5587)
                     /gene="rssA"
                     /locus_tag="ACHKUI_000960"
     CDS             complement(4682..5587)
                     /gene="rssA"
                     /locus_tag="ACHKUI_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6597215.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRTVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            5700..6158
                     /gene="ychJ"
                     /locus_tag="ACHKUI_000961"
     CDS             5700..6158
                     /gene="ychJ"
                     /locus_tag="ACHKUI_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6597216.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVAQFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            6208..7050
                     /gene="purU"
                     /locus_tag="ACHKUI_000962"
     CDS             6208..7050
                     /gene="purU"
                     /locus_tag="ACHKUI_000962"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6597217.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            7210..7294
                     /locus_tag="ACHKUI_000963"
     tRNA            7210..7294
                     /locus_tag="ACHKUI_000963"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7244..7246,aa:Tyr,seq:gta)
     gene            7328..7412
                     /locus_tag="ACHKUI_000964"
     tRNA            7328..7412
                     /locus_tag="ACHKUI_000964"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7362..7364,aa:Tyr,seq:gta)
     gene            7459..7590
                     /locus_tag="ACHKUI_000965"
     ncRNA           7459..7590
                     /ncRNA_class="other"
                     /locus_tag="ACHKUI_000965"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            7844..9043
                     /locus_tag="ACHKUI_000966"
     CDS             7844..9043
                     /locus_tag="ACHKUI_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO6597218.1"
                     /translation="MPARQVCQNFFRGALAPFHKYRQNALLDATIALINGASLTLTSI
                     GRYLPGNAQVKNKIKRVDRLLGNESLHHDIPLIFRNIISMLTSKLSLCVIAVDWSGYP
                     SQEYHVLRASLICDGHSIPLLSWIVPSEKQQNAKIQQAFLNTLSEAVNPKARVIIVTD
                     AGFQNAWFRHIKSLGWDFIGRIRGNKQLHLARKGECWFRRQELQAGNKPEYLGPGTLS
                     RAEYARCDGHFYLHKKEPKGRRNKRSRCGIARPSQIKDARSAAKEPWLIFSSTDDFKP
                     REIMKLYSRRMQIEQNSRDEKSERFGFGLRASYSCSAGRMLVLSLLATLSTIVLWLIG
                     YHAENQGLHLRYQANSIKTRRVISYLRLAENVLRHSPLILKRTVLSTILNHLTRTYQN
                     MVLVYYR"
     gene            complement(9096..9773)
                     /gene="narI"
                     /locus_tag="ACHKUI_000967"
     CDS             complement(9096..9773)
                     /gene="narI"
                     /locus_tag="ACHKUI_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6597219.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(9773..10483)
                     /gene="narJ"
                     /locus_tag="ACHKUI_000968"
     CDS             complement(9773..10483)
                     /gene="narJ"
                     /locus_tag="ACHKUI_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6597220.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(10480..12018)
                     /gene="narH"
                     /locus_tag="ACHKUI_000969"
     CDS             complement(10480..12018)
                     /gene="narH"
                     /locus_tag="ACHKUI_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6597221.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(12015..15758)
                     /gene="narG"
                     /locus_tag="ACHKUI_000970"
     CDS             complement(12015..15758)
                     /gene="narG"
                     /locus_tag="ACHKUI_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6597222.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNCDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIARKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(16151..17542)
                     /gene="narK"
                     /locus_tag="ACHKUI_000971"
     CDS             complement(16151..17542)
                     /gene="narK"
                     /locus_tag="ACHKUI_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6597223.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            17881..19677
                     /gene="narX"
                     /locus_tag="ACHKUI_000972"
     CDS             17881..19677
                     /gene="narX"
                     /locus_tag="ACHKUI_000972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6597224.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            19670..20320
                     /gene="narL"
                     /locus_tag="ACHKUI_000973"
     CDS             19670..20320
                     /gene="narL"
                     /locus_tag="ACHKUI_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6597225.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(20321..21715)
                     /gene="ychO"
                     /locus_tag="ACHKUI_000974"
                     /gene_synonym="iatA"
     CDS             complement(20321..21715)
                     /gene="ychO"
                     /locus_tag="ACHKUI_000974"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6597226.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAASPFDNSNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQLLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            21900..22253
                     /gene="ychN"
                     /locus_tag="ACHKUI_000975"
     CDS             21900..22253
                     /gene="ychN"
                     /locus_tag="ACHKUI_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6597227.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(22297..22992)
                     /gene="chaC"
                     /locus_tag="ACHKUI_000976"
     CDS             complement(22297..22992)
                     /gene="chaC"
                     /locus_tag="ACHKUI_000976"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6597228.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSLKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(23138..23368)
                     /gene="chaB"
                     /locus_tag="ACHKUI_000977"
     CDS             complement(23138..23368)
                     /gene="chaB"
                     /locus_tag="ACHKUI_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6597229.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            23638..24738
                     /gene="chaA"
                     /locus_tag="ACHKUI_000978"
     CDS             23638..24738
                     /gene="chaA"
                     /locus_tag="ACHKUI_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6597230.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            25144..25251
                     /locus_tag="ACHKUI_000979"
     CDS             25144..25251
                     /locus_tag="ACHKUI_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6597231.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(25399..26253)
                     /gene="kdsA"
                     /locus_tag="ACHKUI_000980"
     CDS             complement(25399..26253)
                     /gene="kdsA"
                     /locus_tag="ACHKUI_000980"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6597232.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(26289..27098)
                     /gene="sirB1"
                     /locus_tag="ACHKUI_000981"
     CDS             complement(26289..27098)
                     /gene="sirB1"
                     /locus_tag="ACHKUI_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6597233.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(27102..27494)
                     /gene="sirB2"
                     /locus_tag="ACHKUI_000982"
     CDS             complement(27102..27494)
                     /gene="sirB2"
                     /locus_tag="ACHKUI_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6597234.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(27491..28324)
                     /gene="prmC"
                     /locus_tag="ACHKUI_000983"
     CDS             complement(27491..28324)
                     /gene="prmC"
                     /locus_tag="ACHKUI_000983"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6597235.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(28324..29406)
                     /gene="prfA"
                     /locus_tag="ACHKUI_000984"
     CDS             complement(28324..29406)
                     /gene="prfA"
                     /locus_tag="ACHKUI_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6597236.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(29448..30704)
                     /gene="hemA"
                     /locus_tag="ACHKUI_000985"
     CDS             complement(29448..30704)
                     /gene="hemA"
                     /locus_tag="ACHKUI_000985"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6597237.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30918..31541
                     /gene="lolB"
                     /locus_tag="ACHKUI_000986"
     CDS             30918..31541
                     /gene="lolB"
                     /locus_tag="ACHKUI_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6597238.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            31541..32392
                     /gene="ispE"
                     /locus_tag="ACHKUI_000987"
     CDS             31541..32392
                     /gene="ispE"
                     /locus_tag="ACHKUI_000987"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6597239.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSMGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     MPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            32543..33490
                     /gene="prs"
                     /locus_tag="ACHKUI_000988"
     CDS             32543..33490
                     /gene="prs"
                     /locus_tag="ACHKUI_000988"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6597240.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            33615..35294
                     /gene="dauA"
                     /locus_tag="ACHKUI_000989"
     CDS             33615..35294
                     /gene="dauA"
                     /locus_tag="ACHKUI_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6597241.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(35349..35627)
                     /gene="ychH"
                     /locus_tag="ACHKUI_000990"
     CDS             complement(35349..35627)
                     /gene="ychH"
                     /locus_tag="ACHKUI_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6597242.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35905..36489
                     /gene="pth"
                     /locus_tag="ACHKUI_000991"
     CDS             35905..36489
                     /gene="pth"
                     /locus_tag="ACHKUI_000991"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6597243.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKDKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            36606..37697
                     /gene="ychF"
                     /locus_tag="ACHKUI_000992"
     CDS             36606..37697
                     /gene="ychF"
                     /locus_tag="ACHKUI_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6597244.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDNDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            37887..38063
                     /locus_tag="ACHKUI_000993"
     CDS             37887..38063
                     /locus_tag="ACHKUI_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597245.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(38100..40019)
                     /gene="dhaR"
                     /locus_tag="ACHKUI_000994"
     CDS             complement(38100..40019)
                     /gene="dhaR"
                     /locus_tag="ACHKUI_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO6597246.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDSENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            40247..41317
                     /gene="dhaK"
                     /locus_tag="ACHKUI_000995"
     CDS             40247..41317
                     /gene="dhaK"
                     /locus_tag="ACHKUI_000995"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6597247.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            41328..41960
                     /gene="dhaL"
                     /locus_tag="ACHKUI_000996"
     CDS             41328..41960
                     /gene="dhaL"
                     /locus_tag="ACHKUI_000996"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6597248.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            41971..43389
                     /gene="dhaM"
                     /locus_tag="ACHKUI_000997"
     CDS             41971..43389
                     /gene="dhaM"
                     /locus_tag="ACHKUI_000997"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6597249.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAIKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            43708..45405
                     /gene="treA"
                     /locus_tag="ACHKUI_000998"
     CDS             43708..45405
                     /gene="treA"
                     /locus_tag="ACHKUI_000998"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6597250.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQHQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSIENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            45742..46764
                     /locus_tag="ACHKUI_000999"
     CDS             45742..46764
                     /locus_tag="ACHKUI_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO6597251.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            46764..47744
                     /locus_tag="ACHKUI_001000"
     CDS             46764..47744
                     /locus_tag="ACHKUI_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6597252.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            47741..48499
                     /locus_tag="ACHKUI_001001"
     CDS             47741..48499
                     /locus_tag="ACHKUI_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6597253.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGVNGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            48509..49321
                     /locus_tag="ACHKUI_001002"
     CDS             48509..49321
                     /locus_tag="ACHKUI_001002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6597254.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            49318..50172
                     /gene="modD"
                     /locus_tag="ACHKUI_001003"
     CDS             49318..50172
                     /gene="modD"
                     /locus_tag="ACHKUI_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO6597255.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            50198..52168
                     /locus_tag="ACHKUI_001004"
     CDS             50198..52168
                     /locus_tag="ACHKUI_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6597256.1"
                     /translation="MRLKKRYLCTVLSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYHDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(52218..52472)
                     /gene="ymgE"
                     /locus_tag="ACHKUI_001005"
     CDS             complement(52218..52472)
                     /gene="ymgE"
                     /locus_tag="ACHKUI_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6597257.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            52673..53407
                     /gene="ycgR"
                     /locus_tag="ACHKUI_001006"
     CDS             52673..53407
                     /gene="ycgR"
                     /locus_tag="ACHKUI_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6597258.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLLLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(53409..54020)
                     /gene="emtA"
                     /locus_tag="ACHKUI_001007"
     CDS             complement(53409..54020)
                     /gene="emtA"
                     /locus_tag="ACHKUI_001007"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6597259.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            54120..55034
                     /gene="ldcA"
                     /locus_tag="ACHKUI_001008"
     CDS             54120..55034
                     /gene="ldcA"
                     /locus_tag="ACHKUI_001008"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6597260.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            55129..56865
                     /gene="cvrA"
                     /locus_tag="ACHKUI_001009"
     CDS             55129..56865
                     /gene="cvrA"
                     /locus_tag="ACHKUI_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6597261.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PTGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(56922..57992)
                     /gene="dadX"
                     /locus_tag="ACHKUI_001010"
     CDS             complement(56922..57992)
                     /gene="dadX"
                     /locus_tag="ACHKUI_001010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6597262.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPRARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(58002..59300)
                     /gene="dadA"
                     /locus_tag="ACHKUI_001011"
     CDS             complement(58002..59300)
                     /gene="dadA"
                     /locus_tag="ACHKUI_001011"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6597263.1"
                     /translation="MRVVILGSGVVGVASVWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            59630..61162
                     /gene="ycgB"
                     /locus_tag="ACHKUI_001012"
     CDS             59630..61162
                     /gene="ycgB"
                     /locus_tag="ACHKUI_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6597264.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(61325..62044)
                     /gene="fadR"
                     /locus_tag="ACHKUI_001013"
     CDS             complement(61325..62044)
                     /gene="fadR"
                     /locus_tag="ACHKUI_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6597265.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            62266..63807
                     /gene="nhaB"
                     /locus_tag="ACHKUI_001014"
     CDS             62266..63807
                     /gene="nhaB"
                     /locus_tag="ACHKUI_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6597266.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            63953..64483
                     /gene="dsbB"
                     /locus_tag="ACHKUI_001015"
     CDS             63953..64483
                     /gene="dsbB"
                     /locus_tag="ACHKUI_001015"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6597267.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(64528..65796)
                     /gene="umuC"
                     /locus_tag="ACHKUI_001016"
     CDS             complement(64528..65796)
                     /gene="umuC"
                     /locus_tag="ACHKUI_001016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6597268.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(65796..66215)
                     /gene="umuD"
                     /locus_tag="ACHKUI_001017"
     CDS             complement(65796..66215)
                     /gene="umuD"
                     /locus_tag="ACHKUI_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6597269.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            66478..66801
                     /gene="hlyE"
                     /locus_tag="ACHKUI_001018"
                     /pseudo
     CDS             66478..66801
                     /gene="hlyE"
                     /locus_tag="ACHKUI_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(67008..67454)
                     /gene="ycgN"
                     /locus_tag="ACHKUI_001019"
     CDS             complement(67008..67454)
                     /gene="ycgN"
                     /locus_tag="ACHKUI_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6597270.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(67546..68205)
                     /gene="ycgM"
                     /locus_tag="ACHKUI_001020"
     CDS             complement(67546..68205)
                     /gene="ycgM"
                     /locus_tag="ACHKUI_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6597271.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(68277..68570)
                     /locus_tag="ACHKUI_001021"
     CDS             complement(68277..68570)
                     /locus_tag="ACHKUI_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6597272.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            68812..69213
                     /gene="pliG"
                     /locus_tag="ACHKUI_001022"
     CDS             68812..69213
                     /gene="pliG"
                     /locus_tag="ACHKUI_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6597273.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(69316..69684)
                     /gene="ycgJ"
                     /locus_tag="ACHKUI_001023"
     CDS             complement(69316..69684)
                     /gene="ycgJ"
                     /locus_tag="ACHKUI_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6597274.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRNGKINQTTTKMLFMCRE"
     gene            70204..70899
                     /gene="minC"
                     /locus_tag="ACHKUI_001024"
     CDS             70204..70899
                     /gene="minC"
                     /locus_tag="ACHKUI_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6597275.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEAPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            70923..71735
                     /gene="minD"
                     /locus_tag="ACHKUI_001025"
     CDS             70923..71735
                     /gene="minD"
                     /locus_tag="ACHKUI_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6597276.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            71739..72005
                     /gene="minE"
                     /locus_tag="ACHKUI_001026"
     CDS             71739..72005
                     /gene="minE"
                     /locus_tag="ACHKUI_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6597277.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            72156..72329
                     /locus_tag="ACHKUI_001027"
     CDS             72156..72329
                     /locus_tag="ACHKUI_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597278.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            72331..72675
                     /gene="ymgG"
                     /locus_tag="ACHKUI_001028"
     CDS             72331..72675
                     /gene="ymgG"
                     /locus_tag="ACHKUI_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO6597279.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            72679..73014
                     /gene="ymgD"
                     /locus_tag="ACHKUI_001029"
     CDS             72679..73014
                     /gene="ymgD"
                     /locus_tag="ACHKUI_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO6597280.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(73073..74592)
                     /locus_tag="ACHKUI_001030"
                     /pseudo
     CDS             complement(73073..74592)
                     /locus_tag="ACHKUI_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT        18672 a        19268 c        18251 g        18592 t
ORIGIN      
        1 aggactgtta aatgcctgga gcctggcggg agaacaccgc cactggatga tccctctcag
       61 aaagggagcg caatatgaag agattagaaa actgggtaaa ggcgatcatc tggtgaagct
      121 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgagg tgacagcccg
      181 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      241 catacgcttc cccaggacgt ataccggagc tgatgcgcga tcttgcaagt atgggacaac
      301 cagtgaaatt accgacaaga agggaaaggg ccttcccgag agtggtaaag gagaggccct
      361 ggaaataccc cacagccccg aaaaagagcc agtcagttgc ttaactgact ggcattaccc
      421 cttctggggc cgttttttgt tttcccaaaa caacttttcc ataaaccggc agcattgtcc
      481 ttcatcatat ttgcgacgat gtataacgcc tacacacagc gatattgtac tttacaggtt
      541 acaagtcaat gtcggtgtat aagcgctctg tgaggcgtat agcggcgtta aaaaactgcc
      601 gagaagggtg tatagcccga aagaagtgcg taaaacgaac tgacaggata aaagcgccct
      661 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      721 ttaatgagcc gacttctaac gcttctttat agtgtttacg gcatacagaa acgtagcgtt
      781 cattaccacc aataaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      841 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      901 cggaccacgc cagtaagtat tgactgccaa taaataattc acctcgaaaa tcggtacgta
      961 aaccataaca aagtacaggt atatcgagtt gatcgacaac ctccgataat tcatatactt
     1021 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
     1081 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
     1141 acaaacctat acgcgaactg actttcccgg caccaaaacg atcatcaatt tctgccgtat
     1201 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
     1261 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
     1321 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
     1381 agtatgcaca gaagtggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1441 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactgccggt aactattcac
     1501 aatctttaac ctgttgcgca tgtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1561 ctaagtccct gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1621 tcgtaaacac ggctaataca gaagactgaa aggtcgtcag cctacgatta tctccccata
     1681 aaatgtgaca tgaatcagga agtttaaacc tcacgtgctg cgaaatcatc ggtgcaaata
     1741 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1801 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1861 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1921 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1981 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     2041 aagcgcggct gctgctgaag ttgaagagcg cactcgtaaa ctgcagcaat atcgcgaaat
     2101 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     2161 tggcaccaaa gctaagcgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     2221 cgaaactaaa acctggactg gccagggccg tactccagct gtaatcaaaa aagcaatgga
     2281 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     2341 atgctaaaaa tcccgccatc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     2401 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggaa aacaccgata
     2461 cggatgttac ttcttaatgc ccatctcgtc ttcaagccag gctttaaatt ccgtgccaag
     2521 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2581 gtcatggctc ttccctttca tatgataggc ttcaaccgtt tctttttcga tcagcatatc
     2641 aattgcgtcg gtgagctgaa tttcatcacc agctccagga ggggtttttg ccagcaacgg
     2701 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2761 cgcttttggt ttttcaacca cgccaaccat cggtacgctt tcacccggcg ctaattcaac
     2821 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2881 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgataaatc
     2941 ggattcatat tcatccagaa taacgtcagg caaaataaca gctaccggtt catcacccac
     3001 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagcccct gacgaacttg
     3061 cataatagtc acgtgtggcg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     3121 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     3181 tttagatgag tgagtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     3241 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     3301 cgccggcaac atcctggttc ctaatcccgc tacggggata acggcttttt tgactttcgt
     3361 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3421 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3481 tagcataatt aagacaatta cttatacgaa tagcgacgat agtaccacca gccgacatta
     3541 gcaggtaatg caaatttagc ccgtgttatc gtttgctcat tctgcagaca acatcaagcg
     3601 cagccgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3661 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctgatg
     3721 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3781 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3841 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3901 ctgttcctgt aataatccgt taaacaatac gcgtaataac aaggcagcaa gtacgccatt
     3961 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     4021 aatatcaagc accaggccgg gtttgtccgc ggcaactaat tgacgataat taacccggca
     4081 ttgggaaatc acctgctgaa ctggcggttg tagttcctgt aataatttcg ccgctgcagc
     4141 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcggga
     4201 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     4261 aaccggtttc agcaaaacat cttcaacacc cagacgtaac gctttggcaa tatctgccat
     4321 attttcagtg gcagatatca ccagaactgg tgtctggtca cctctattgc gtatatgttc
     4381 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4441 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4501 caatgaggaa aaccacgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4561 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4621 gtggcacgct attgccacct acgcctgtca gaattaactt aagtgtagat aaaaattctg
     4681 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcttttcc actgccagct
     4741 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4801 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta agtcggttct
     4861 ccagcacctg gatagaagtg gtcataatct ctgttgccgt tggcgctgtc accgtccgac
     4921 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggcaga gaatcaccat
     4981 tctcgctatt ttcttcactg acattaaagg agagcaaatc ttgttgcatc aaatgagcat
     5041 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgtg agggaaatag
     5101 gaattgggtt aactacagcg ccatcaacca gccagtagcc gttatgcgca acgggtgcca
     5161 ttagtccagg aatactgcat gatgcacgaa tggcaagatg gagatcgcct tcagtaaacc
     5221 ataattcacg cccagtactt aaattggtgg caacagccgc gaagcgacgg gaacaatttt
     5281 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     5341 acccaccgcg ctgccaggag agatccatca ggcgtaaaac atcccaataa ctgaaagagg
     5401 tcacccaatc ttccagcgca gataatcgat cgcatgcata ggcagcgccc accagcgaac
     5461 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaatta
     5521 cgccaatatg cgaccaacct cttgccgcgc cagatcccag cgccagccct atttttatct
     5581 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5641 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5701 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5761 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5821 ttgtgatgca agacgcagat tatttaataa agacctggca tccatcttgt ggagcagcgg
     5881 cgttacgtgc cgaattgatt gccggatttg cgcatactga atggttgggc ttgaccgtat
     5941 ttgaacattg ctggcaggac ggcggcaata tcggatttgt cagttttgtt gctcaattta
     6001 ccgagggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     6061 aatggtacta tattgacggt acacgtccgc agtttggccg caacgatccc tgcccttgtg
     6121 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     6181 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     6241 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     6301 ttaaatatcg tgcagaacaa tgaatttgta gatcatcgta ccgggcgctt ttttatgcgc
     6361 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6421 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt aattctggtc
     6481 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6541 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcggttt
     6601 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6661 atggcggatg ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6721 gtattaacgc cggaatttgt ggcacgcttc ccgaataaga tcatcaatat tcaccattca
     6781 ttcctgccag cgtttatcgg cgcacgtcct tatcaccagg cctatgagcg tggagtgaag
     6841 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6901 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6961 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcgca gcgcgtcttt
     7021 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttagctttaa
     7081 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     7141 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     7201 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     7261 gacttcgaag gttcgaatcc ttcccccacc accatctcaa gcattaccta caatatgaat
     7321 tacccctggt ggggttcccg agcggccaaa gggagcagaa tgtaaatctg ccgtcatcga
     7381 cttcgaaggt tcgaatcctt cccccaccac catcactttc aaaagcccct gaattctcaa
     7441 acgaatccgc aattaaatat tctgcccatg cggggaagga tgagaggctt cgaccaaggt
     7501 tcgactcgag tgccagcgag agagcgttgc cgcaggcaac gacccgtagg gcgaagcgcg
     7561 cagcgctgag taatccttcc cccaccacca tttcttcctg aaatactcaa aacctgttac
     7621 tccgagctaa ttctgcccaa tggcaccgcg tttaccggac ctaaaatatc ctcccctgta
     7681 ggtcggataa gcgaagcgca tccgacaact ccccccgcac cgtgcccata aaaaaaaccc
     7741 gccggagcgc tgaggaatcc ccagtaatgg gcgggtaaaa aaagacaggc atagagtttt
     7801 gtgatctact gacttgtgca accaattcaa tgagatctcc tctatgcctg ctcgccaagt
     7861 atgtcagaat tttttccgcg gtgctttagc accatttcac aaataccgac aaaatgcttt
     7921 gttggatgcc actatcgcac taatcaatgg cgcatctctg acgctgacca gcatcggacg
     7981 ttatttaccc ggtaatgctc aggtcaaaaa taaaataaaa cgtgttgatc gtctgctcgg
     8041 taatgagtct cttcatcacg acatccccct gattttcaga aacattattt ccatgctgac
     8101 gagcaagctg tcactgtgtg tcatcgccgt tgactggagt ggatacccct ctcaggagta
     8161 tcacgtactc cgcgccagcc tgatttgcga tggacattcg attccattgc tgagttggat
     8221 agttccttcg gaaaaacagc aaaatgcaaa gatacagcaa gcctttctta atactctttc
     8281 cgaagcggta aacccaaaag caagggtaat catcgtcacg gatgcagggt tccagaatgc
     8341 ctggtttcgg cacataaaat cactcggctg ggattttatt ggccggatac gaggcaataa
     8401 acaactgcat ctggcgagaa aaggggaatg ctggttcagg cgtcaggaat tacaggccgg
     8461 taacaaacct gaatatctgg ggccggggac gctttcccgg gcggaatacg cccgttgcga
     8521 tggtcatttt tatttgcaca aaaaagagcc gaaaggccgg agaaataaac gttcgcgttg
     8581 cggtatagcc agaccatcac agataaaaga tgccaggtcg gcggcaaaag aaccatggtt
     8641 aatattcagc agcacagatg actttaaacc gcgagaaatc atgaagttat acagccgccg
     8701 aatgcagatt gagcaaaact ccagggatga aaaaagtgag cgttttgggt tcggtctgcg
     8761 agccagttac agctgttcgg caggaagaat gctggtactg agcctgctgg caacactgag
     8821 cacaatagtg ctgtggctta ttggctatca cgctgaaaat caagggttac atcttagata
     8881 tcaggccaac agcattaaaa cgcggcgggt aatctcgtat ctgaggttag cggagaatgt
     8941 cttacgacac tccccgctaa ttttaaaacg aacggtgcta agcaccattc ttaatcacct
     9001 caccagaacc taccagaata tggtgttggt ttattatcgc tgatttatgg ggatccctca
     9061 gcgccgaagc agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctga
     9121 tacttacgcg tcagatactc caccgggacg ctccagatgt gtaccagacg cgagaacggg
     9181 aacagcagga ataacgtcat ccccaacacc aggtgcagac ggaagataaa cgccacgcca
     9241 tcaagatgtt gagaagcgcc accgtggaag gtcactacag actgcgccca gccaaccagt
     9301 ttcatcatct cgctaccgtc catatgctgg gcggagaacg gaatggtcag caggcccagc
     9361 gcgcactgga taacgagcag cgacaggatc aagatatccg ctccggtagt cgttgcacgc
     9421 acgcgtgggc tgaacagacg acgtttcaac aacagcacgc cgccaatcag acacagtacg
     9481 ccgctggcac caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct
     9541 tcatacatcc agtgcggcgt cagcataccg aagaagtgac cgacaaaaat ccccagaata
     9601 ccgatatgga acaggttcga cgccagattc atccctttgc gatccagcat ctggctggac
     9661 gccgcacgcc atgtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc
     9721 gccccggcta tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct
     9781 gtcctccggt ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat
     9841 gagcagtgat tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt
     9901 cccagactgc atccagcgcc tgcggcgtat cgtcgcgcgc ttcatcggca attttttccg
     9961 ccactttgtc gctgtcgatt gcggtattcg ccagtttcag cagcagatca aacagcacgg
    10021 cataacggct ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc ggcgcaatat
    10081 ctttcaaacc ctccacggct tcgctttgcg gcagctgcgc caggtactcc agatacagcg
    10141 gcagatggtc gggcaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca
    10201 gcagatccac catcgcctgg ccgcggtcac gggattcacc gtgaacatgt tcaaatagca
    10261 acagtgacgt ggcgcggcca cggtcgaaca gttcgctgta ctgcgcctgg gcatcgagcg
    10321 gatccatcgt cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca
    10381 gatttttcga cgcggcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat
    10441 ccggatattc aaggagacgc gatacaatca cgagttcgat catgggtgcg gctccgtttt
    10501 gctggttaca tcgatggcat cgatacggcg gctgttgaac agattgaatt tggtatctga
    10561 accgtggcag ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg
    10621 cgccagttca cgatgactgc tcggcaccac aaagcgatct tcgtagttgg caatagccag
    10681 ataacgatac atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt
    10741 atcgacttta ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt
    10801 cagcgcgcgc agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac
    10861 cggaatacgc aggctttcga cgtctggcag aatgccgttg ctgcccagtt cacccgcgtc
    10921 tgccgcagac tgaatcggcg acagaggcgg cacgtaccag accatcggca gtgtgcgata
    10981 ttccggatgc agcggcagcg ccagtttcca ttccatcgcc attttataaa ctggcgactg
    11041 ctgcgcagcc tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt
    11101 cggatcgttc ggatcgagga acacatccag ctgacgctgg tagagatctt tctcgttctc
    11161 ggtacttgcc gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat
    11221 acgaccgaca caggtttctg aacatattgt cggctggccg gcttcaatac gcggatagca
    11281 gaagatgcac ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca
    11341 tccggtgatg cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc
    11401 ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcagaca
    11461 gtgttcgcac aggcgcggca aatacatcat gaaggtgttt tcgaactggc tatacatcgc
    11521 cttctggatg ttgtcgaagt tcttgtcttt cgccagcttg tcaaactcac cgcccaggtc
    11581 atcttcccag ttcggccctt tttcgatttt cgccatccgt tcgccggtaa tcagcgaacg
    11641 cggacgggca atcggctgtg atttgctgcc ttccggcgcg gtatgcaggt tctgataatc
    11701 aaaatcgaac ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt
    11761 acccagcagc atggcacggt tacccatgcg cggctgcagt ttgccgttga ttttacggat
    11821 ccagccgcct ttgtattttt cctggttttc ccagtcagtc gggaagccct ggcccggctt
    11881 ggtttccacg ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt
    11941 acaggtcact gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac
    12001 ttgtgaacga attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac
    12061 cagtcaatgt tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg
    12121 ccgtagtagt taaagccata tgccagatgg gcatagccgc cgatcatatg cgtcggtttc
    12181 ggcgtgatac gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca
    12241 ggcaggttaa cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc
    12301 tggctgacaa ccgcacgggc agtcagtgca ccgttgctgt tgaagacttc aatccagtcg
    12361 ttatcggcga tacccagctc tttggcatcg gcttcactca gccagaccac cggaccaccg
    12421 cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg
    12481 tgtggcgtca ggaagttgag cgctttttcc gggttgccat tggatttctg gcccatcact
    12541 tctttcaccg aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca
    12601 cgcatccatt ggtgatcctg atacagctgc tgacggccag agagcgtacg ccacgggatc
    12661 agctcgtgaa cgttggtgta accggcgttg taagaaacgt gttcatcttc aaggccagac
    12721 catgtcgggc tggagatgat tttgcgcggc tgtgcctgaa tatcgcggaa gcgaatcttc
    12781 tcgtcttctt tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca
    12841 gcccaggctt ttacagctac ctgaccattg gtttccggtg ccagtgtcag gatcatctct
    12901 gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg
    12961 tagttgagct tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg
    13021 ccgttaccga ttttctccat cagcgggccg atagaggtaa agcgttcgta agttgccgga
    13081 taatcgcgct ctacgaccat aatgtgcggt gcagttttac ctgggatcag gtcgcattcg
    13141 ccttttttcc agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcgtgctgg
    13201 ataggcagcg tgacgatgtc agtttcttta cccagatgac caacgcacac ttcggagaat
    13261 ttcctggcga tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc
    13321 gccgcagaca gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg
    13381 taccaagtcg ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag
    13441 tccagcgtca ccaccagatc cagcttgcct tccagaccgt tgtcctgcca gtccacttct
    13501 tccggcttca cgccgccctg ctgccccagg tctttgccct ggataccgtg ctccgtcccc
    13561 agcaggtact tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc
    13621 cagatgaaca gattacgcgg gtggttttta ccgttttccg gctgttccgc agcgaaacgg
    13681 atagaaccct ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttct
    13741 gcttcgcgag cgattgtcag cgggttagtg cctaactgcg gcgcagacgg cagccagccc
    13801 atgcgttctg cacggacgtt aaagtcgatc aagtgtccgg tatagcgaga tttatccgcc
    13861 atcggcgaca gcagttcttc cgcagtcacg gtttcataac gccactggct ggagtggtta
    13921 tagaaataag aggtactgtt catgtgacgc gccggacgct gccagtcgag ggcaaacgcc
    13981 agcggctgcc agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagcca
    14041 ccgccgctct gaccgacaca gccgcagaaa atcagcatat tgatcagacc acgatagttc
    14101 atgtccaggt gataccagtg gttcagccca gcgccgacga taatcatcga acgaccgtgc
    14161 gttttatcag cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg
    14221 ccggtaatct gctctgccca ggcaggggtg taagctttca catcgtcata gctggttgca
    14281 cagttaacgt cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg
    14341 gtggtcacca gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagcttatgc
    14401 agcagcacgt tttccagttc cactttgttg aagtgttccg tgccatcgcc accaaagtac
    14461 gggaagccca cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt
    14521 tcttcgccag ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg
    14581 aagccaatag aaccgttcgg cgcaaccatt tcaccattgg tattaaaggc gacagttttc
    14641 cattccggat tgttttcctg gcccagtgca tccaccagat cagcagcgcg cagcatacga
    14701 cccgcagcgt agtaaccgtc gcgctcttcc agcatcacca gcatcggcat gtcggtgtag
    14761 cggcgcacat agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt
    14821 acgtgaccca ttgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga
    14881 tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggttcctttg
    14941 taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg tctgcggcac gtttgaaccc
    15001 caggcgatga tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctca
    15061 ccccaggttt gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa
    15121 gtaccgccaa tcagcgagag atagcgcgca cccgatgcgt aagaaaccat cgacattgcc
    15181 ggaattggcg agaagccagc gacacggtcc ggaccgtaat ttttgatggt gtaaacgtta
    15241 gatgcggcga tcagttcgtt cacttcctgc caggaagaac gaacaaaacc accgcgtcca
    15301 cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca
    15361 accggatcgc tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc
    15421 atcgggtatt tcaggcggtt agcgctgtaa agataccagg agtagctggc accgcgcggg
    15481 cagccgcgag gttcatggtt tggcaggtcc gggcgagttc gcggatagtc agtctgctgg
    15541 gtttcccagg tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag
    15601 tttaccccgt gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc
    15661 tcccagtcac agttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc
    15721 tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct
    15781 gtcggccctc tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca
    15841 gcgtgggaat tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctggaa
    15901 taccccttta ggataattaa catttatagc ataacagact gtaaattaag acgttttaca
    15961 cctgtggtta tttaaaccat taagattttt atgcagatgg gtattaagga gtattcccca
    16021 tgggtaacat attaatgact cttaatgaaa tccagcgaaa taagccatac aacattagta
    16081 tgttgtaact aaatgacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa
    16141 atattacgat ttatttttta gaatgccgac catataccgc ccaggtaatc accacgcagg
    16201 cgatatagaa aatcaaaaat actttcattg cgccgactgg cgaacccgtt aatgccagcg
    16261 agctaccaaa cgctttcggg ataaagaagc caccaatcgc gccaatcgca gagatgaaac
    16321 ccagcgccgc cgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca gaaccccctt
    16381 ctgctttcac gcgatccatt gtcagtttac ggaagatcac ggaaatcatc tggaaagtgg
    16441 aaccactacc cagcccagct gtcaggaaca gcgccaggaa gactgcgaag aacgccatga
    16501 agcttccgcc ctgtccgtca gtcggtaagg tcaggaacag caggccgctg aaaatcgcca
    16561 tcaggataaa gttcaccagc gtgacacgag ttccgcccag acggtcagat aatgcaccac
    16621 ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa agcgtattgc agaatctgaa
    16681 catccgggaa ctgcgttttt gacagcatcg caaagcccgc ggagaagccg atgaaggagc
    16741 cgaaggttgc cagatacagc aggctcataa tccacagatg accccgtttg agtaccggca
    16801 actgctcctt gatggaggct ttcgaggtag caagatcgtt catgccaaac cacgccgcaa
    16861 tggtgaagat ggcaaggaac ggcacccata tccaggacgc attcgccaga tacagctcag
    16921 tcccatccgg ctgtttgacg ccctggctac caaatactgc gaaaatcgac agtgatacca
    16981 ccagcggagc aaccaactgc atgacgctga cgcccatgtt gcccagacca ccattcagac
    17041 ccagcgcgcc accctgcttc tgtttcggaa agaagaagct gatgtttgcc atactggatg
    17101 cgaagttcgc gccagcaaag ccgcacagca gagaaatgat gatgaagacg ctataaggcg
    17161 tggaggtatc ctgcacggca aaacccagcc agacgcaagg aataatcaga ataccggtgc
    17221 tgaacgccgt ccagcgacga ccaccaaaga taggaaccat aaaggagtat ggaacacgta
    17281 ataacgcgcc agaaaccgaa ggcagcgcag tcaacataaa tagctgatcg gtcgtaaaat
    17341 taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg
    17401 ccagcagcag acagggaacg gaaatccaca ggttgcggct ggcaatacgt tgaccgcgtt
    17461 gttgccagaa cgcaggatct tccggtcgcc aatctgtaat gacagctcca gtagcccttt
    17521 cgggggcgga tgagtgactc atagacacct ctgatactcg tttcgatgtc tgccacctta
    17581 gtgtctgtag ctaaaggcaa tttgatgtaa atcaaacgat aagataactt tatctatcat
    17641 tgatatttat cattatccat agtgagtaca gtgatttcat aaaaattatg agatttttca
    17701 cggtgctgta aaatccctac ccttaccgat gtaaagcgac taaccacacg gcaaataagg
    17761 agtaactctt tccgggtatg ggtatacttc agccaatagc cgagaatact gccattccag
    17821 aatgtatcat cacattcatt aaggttattg ctcatttaaa gcctgaagga agaggtttac
    17881 atgcttaaac gttgtctctc tccgctcacc ctggttaatc aggttgcgct tattgtgttg
    17941 ctttctactg ctattggact ggcagggatg gcggtttctg gctggctggt gcaaggcgtt
    18001 cagggcagcg cccatgcgat caacaaagcg ggatcgctgc gcatgcaaag ttaccgtctg
    18061 ttggcagcag tgccattaag cgagaaagac aagcccttaa ttaaagagat ggaacaaaca
    18121 gcatttagcg ccgagttgac tcgagcagca gaacgagacg ggcaactggc gcaattacag
    18181 ggtttacaag attactggcg taatgaactg atccctgcac tgatacgtgc acaaaaccgc
    18241 gaaacggtgt cagcggatgt cagccagttt gttgccgggc ttgatcagct ggtatctggt
    18301 tttgatcgca ccacggaaat gcgcattgag acagtggtgc tggtccatcg ggtaatggcg
    18361 gtatttatgg cacttttact ggtattcact attatctggt tgcgggcgag attgctacaa
    18421 ccgtggcggc aactgctggc aatggcgagt gccgtcagcc atcgcgattt tacccaacgc
    18481 gcaaacatca gcgggcgcaa cgaaatggcg atgcttggaa ctgcgttgaa caatatgtct
    18541 gcagaactgg cggaaagtta tgccgtactt gagcagcggg ttcaggagaa aaccgccggg
    18601 ctggagcata aaaatcagat cctctctttt ttatggcagg ctaaccgccg tttgcattcc
    18661 cgcgccccgc tgtgtgaacg cctgtcacct gtactcaacg acttacagaa tttaaccctg
    18721 ctacgtgata tcgaactgcg ggtgtatgac actgatgatg aagagaatca tcaggagttt
    18781 acctgccagt cagatatgac ttgtgatgat aaaggctgcc agctctgccc gcgcggcgta
    18841 ttacccgttg gcgatcgcgg cacaaccctg aagtggcggc tggctgacac tcatacgcag
    18901 tacggtattt tgctggcgac cctgccgcag gggcgtcatc ttagccatga tcaacaacaa
    18961 ctggtggata ccctggttga acaactcacc gccacgctgg cgctggatcg ccatcaggaa
    19021 cgtcagcaac agttgatcgt gatggaagag cgtgccacca ttgcgcgcga actgcatgat
    19081 tctattgccc aatctctctc ttgcatgaag atgcaggtga gttgcttaca gatgcagggc
    19141 gatgcgctgc cagaaagcag ccgcgaactg ttaagtcaga tccgtaacga actgaatgca
    19201 tcctgggtgc agttgcgtga attgctcacc acattccgtt tgcagctcac cgagcctgga
    19261 ttacgtccgg cgctggaggc gagttgcgaa gagtacagcg ccaaatttgg cttcccggtg
    19321 aagctggatt atcaattgcc gcctcgtctg gtgccttcgc atcaggcaat ccacttgttg
    19381 caaattgccc gtgaggcatt aagtaacgcc ctcaaacatt cgcaagcgag tgaggtcgtg
    19441 gtgacggtgg cgcaaaacga taatcaggtc aaactgaccg tccaggataa cggctgcggc
    19501 gtgcctgaaa atgccatccg cagcaatcac tacggcatga taataatgcg cgaccgtgcg
    19561 caaagtttac gaggcgattg ccgcgtccgc cgtcgtgaat caggtggcac cgaagtggtg
    19621 gtcaccttta ttcccgaaaa aactttcaca gacgtccaag gagataccca tgagtaatca
    19681 ggaaccggct actatcctgc tgatcgacga tcacccgatg ttgcgaactg gcgtaaaaca
    19741 gcttatcagt atggcaccag atatcactgt ggttggcgaa gcgagtaatg gcgaacaggg
    19801 tattgaactg gcggaatctc ttgatcccga tctgatcctg ttagatctca atatgcccgg
    19861 catgaacggt ctggaaacgc tggataaact gcgcgaaaag tccctctccg gtcgcattgt
    19921 ggtattcagc gtctctaacc atgaagaaga tgtggtcacc gcactgaaac gcggcgcgga
    19981 tggctatctg ttaaaagata tggaaccgga agatctgctg aaagcgttac atcaggcagc
    20041 tgctggcgaa atggtattaa gcgaagcatt aacgcctgtt ctggccgcca gcttgcgcgc
    20101 aaaccgtgcc actactgagc gcgacgttaa ccagttaacc ccacgcgagc gcgatattct
    20161 caagctgatt gcccagggtt tgccgaacaa gatgattgcc cgccgcctgg acatcaccga
    20221 aagcacagta aaagtgcacg tcaagcacat gctgaagaaa atgaagctca agtctcgcgt
    20281 ggaagcagcg gtatgggtgc atcaggagcg cattttctga ttacggttcc cagcgcagtt
    20341 cgtcgtttga caatgcgtcg aacggttcga caagcgttag cgtgatctca ttggaggaga
    20401 cacgctgccc ctggttatct tccaccacca ctgacaatcg ccagtgattg cttgcgcctt
    20461 ccccgttttg ccagtcaggc atgatcagcg tccagccctc ctcactgttg gcttgtgcgc
    20521 ccggcgtcaa acttaatatc tgcgtatcgc cctgccaaat cagttgccga ataccgtaac
    20581 gactgcggat ttgtaatttc agcggcactg tttcgccagg ttttagatcc cacggaggtg
    20641 tcgccagaaa caccgttaac gtttttcgct gtcggtactc aagagtcggc agattatttc
    20701 gctgcggatt gtcatagcga ctaccacgta acgactgact ttcggcaacc tcgcccgcag
    20761 aaagttgttt tttgagcggt acaccaaagc ggtagttaag attcagcccg aggttatttt
    20821 gattctcgcc actttcaccc tgtttatgct gggccgtcac agtgactaag ggcacagggg
    20881 tgtaatttaa tcccagactc aacgcgacgg gattgtgata acccgtacca gagttaaaca
    20941 aatcgacacg atcaccaaaa tactgttcta cgctgacact ggtattgagg agttgataga
    21001 acggcatgcg catccgggct gtcaggtcgt acccgcgcgc catccgttgt tcctgcgtgg
    21061 ctgtctgttc atgccatgca gcaaacggct gataaaagtt tgccgatagt cgcaaatatt
    21121 cgccccacgc ttcagcgcca aagcccgctc gctgaagatt ttcgtccagc aagttgtcat
    21181 aaaaagtgtt ataacccacc agccagctgc cgcgcgccca gcgttgccca acgcccacat
    21241 tgctcaccag cccatcatcc tgctgagtaa gaccaagctg gctccaggtg agataacgat
    21301 cattatcttg taacggcaca aaccagcttc cacgactgcc ggtgaaatgt ccttcgttat
    21361 ccactttgac gtcaacactg gcatttcccc acggtgatag ccaggactct acgtgctgat
    21421 taacctgttg actaagcgca tcgcggactt ttcccaatgc gaaagctttt gcctgctcgc
    21481 cagtatccag cccgttatca ttcatactgg tttcgccgaa atctttcaca atttcagcaa
    21541 agtgtttttc cccatcatga ttttcaggtg ccatgcctaa atccggcaga ccatcattgc
    21601 tattatcaaa gggacttgcc gctttttgct cgaaggtaga ttgtgcgtta gctgtaccgc
    21661 ctgccgccag caagagtaaa taaaacggaa taatgcgagg aacgaaacgg ctcaaaacgt
    21721 cggtaacttc cggtaaaaat gaaaaaccat agccttaaag aataacgatt actggcacct
    21781 tttgtcgtat tttctcgcgc tttcaggaaa atccttaaaa atgtgaccga ttggtcagac
    21841 gctgcagaag cagtaagata gaagatgagt gtctaattcc ctgaatacag gagtaaataa
    21901 tgcaaaaaat cgtgatcgtt gccaatggcg caccttacgg gagcgaatcc ttgtttaaca
    21961 gcttgcggct ggccattgcg ttacgagagc aggagaacaa tctggatctg cgtctgttcc
    22021 tgatgtctga tgcggtcaca gccgggttgc gcgggcaaaa accaggggaa ggctacaaca
    22081 ttcagcaaat gctggagatc cttaccgctc agaatgtacc ggtgaaattg tgcaaaacct
    22141 gtaccgacgg gcgcgggatt agtacacttc ctctgattga tggggtggaa atcggtactc
    22201 ttgttgaact ggcgcaatgg acgctgtcag ccgataaagt gctcacattt taatgtttga
    22261 gggggcgtct gacgccccac tatgagggaa gtttactcag gcgaatcccg gccgtaacac
    22321 accatccgga taattctccg ccagcagttt ttttagcgat accagcaaat cattcagccc
    22381 atcgtcctgc attcccagtt tgataagctc ctgttccagt gaaaacaggt attgtgcatt
    22441 ggttcccagc ggaccgctcg ccgccgcaat caacggcgct atgacctgag cgcgagtgtc
    22501 agattcatat tctggatgtc gcgggtccat aataaacaca atggcgttta ctgtgcgtcc
    22561 atcatcaaga tcaagctgac accaggttgg cagataacag ccggtaatca tctcgcgctt
    22621 ccacagcagg gttagttcct gctccagcgt ctcttctggc agtcgatagg cgacgcctgt
    22681 ggtgcgtccg ccctctttca gtgcaagcat tcgtcccggc tggtgcgcag tcccgcgccc
    22741 ggcggtcagg cgcaggcaaa acgcgcgatg ccatccaacc agtgtaccgg tgcacgactc
    22801 ggtaaactcc agtgccggat tccacatcaa cgatccataa ccgaagatcc acaccggtcc
    22861 ctcatcaggt cgacaagcca gcgtcgccgc cagcgaagcc gcccgttgtt ctgctgacca
    22921 taagagtgat tcttcaatgg caccaaatgc cgttttacaa tcggcattca tcaagaaatc
    22981 acgcgttatc accttacacc tccgccactg catacttccc tgcgacaact ttttggtgtc
    23041 ttccggacat ttttactgct cattttgaaa ccactaacgg gacaatatgc aatccacgga
    23101 ccacttgcaa ggcaacaccg cacgaatagc tttagtttta cgatttttta tgccatttat
    23161 catcatcccc tttggcatat tcatgcttca cagcagccca ggccacttta tgcgctgttt
    23221 cttcgcgact ggcgtcatca cgccgatctt ctttatcttt atattgatcc catgcgctgt
    23281 tgaacgcttc tttatagata tcctgggcat gagacggaag cacgtgcttt acgctttccg
    23341 gcagatcgct tttcgtttta tacggcatcg ttgactcctt ttacaaagtt gaacattaag
    23401 tgtggtcagc aatatctact cttgccaaaa cataagaatt ttcatgtcag actcttctta
    23461 agaagtgcta aaacaaacat tgtctaaacg tattacttat ttttatgccc aaatgagtaa
    23521 aaaatcgaca gcggggtaaa aattcgtaaa atgttaccga tatccagaca atttctgtta
    23581 tttttcctac ctatttttta cattttcgat aaattcatct gcaaagggat aaccataatg
    23641 tcaaatgctc aagaggcggt aaaaacccgc cacaaggaga cttcgcttat tttcccggtt
    23701 ctggcgctgg tagtgctgtt cctgtgggga agcagccaga cactaccagt ggtcattgcc
    23761 atcaatcttc ttgcgcttat tggtatttta agtagcgcct ttagtgttgt ccgtcatgcg
    23821 gacgtattag cccatcgcct gggagaacct tacggttcgc ttattcttag cctttcagtg
    23881 gttattcttg aagtcagttt gatttcagct ttaatggcaa ccggcgacgc cgcgccaacg
    23941 ctaatgcgtg atacgctcta ttcaatcatt atgattgtta caggtgggct ggttggcttt
    24001 tcattattgt tgggcggacg taagtttgcc acccaatata tgaatctgtt tggtatcaag
    24061 cagtatttaa ttgcgctgtt ccccctggcg ataatcgtac tggtatttcc aatggctctg
    24121 cctgcggcga atttttcaac aggtcaggcg ttactggtag cattaatttc tgcggcaatg
    24181 tatggcgtat ttttgctgat ccagaccaaa acgcatcaaa gtttgtttgt ctacgagcac
    24241 gaagatgaca gtgatgatga cgacccgcac cacggtaaac cgtctgccca tagcagcctg
    24301 tggcatgcca tctggttgat tgtccatctg attgccgtta ttgcagtcac caaaatgaac
    24361 gccagcccgc tggagacatt gctcgacagc atgaatgcgc ctgtcgcctt tactggcttc
    24421 ctggtggctc tgttgattct gtcgccggaa ggtttaggtg cgttaaaagc agtgttgaat
    24481 aaccaggtcc agcgcgcgat gaatctgttc tttggttcag tgttggcaac catttcgctt
    24541 accgtacctg tcgtcacgct aattgccttt atgacgggta acgaattgca gtttgcactt
    24601 ggtgcgccag aaatggtggt gatggtggcc tctttagtac tgtgccatat ctccttctcc
    24661 accggacgaa ctaacgtgct caatggcgca gcgcatctgg cactgtttgc cgcctatttg
    24721 atgacgatat ttgcctgaca gttatatctc ctccgatgac ggaaatcggg ggagataata
    24781 taaattcctg cttattgtat ttcttcatac tacctggaca ggtcgttaat cagaattgcg
    24841 agcctgtacc acaacctgca cgaaaccact gcaacagctg ttgaaaagcc ggaatgtact
    24901 cttcaatgca aacatttcag ggatgaagtg aagatgaatt gcgacgtagt atccacacca
    24961 agccggcgtt atccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt
    25021 gctggaaaga gaaaaccccc gcacgttgag aggtaaaacc tgacaacaga acgggggcta
    25081 atcttgaacc atcaacaatc caagaatagc cgcaaaatgt tgtcattaca acaaggcggc
    25141 tatatgacgc tcgcgcagtt tgccatgatt ttctggcacg acctggcagc accgatcctg
    25201 gcgggaatta ttaccgcagc gattgtcggc tggtggcgta accggaagta aggtgaaaac
    25261 gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg gtaacagcct
    25321 gcggcctttt ttgtatccgg cacccataaa aaaagtctta acgcagaacg ctaagacttt
    25381 atttttcaaa caaaaagatt acttgctggt atccagttct tcgaaacctt tcaccagatc
    25441 atcaatcgct ttcatttgct tgaggaacgg ttccagttta gccagcggca gcgcggatgg
    25501 accatcacat ttcgcatgtt ccggatccgg atgcgcttca ataaacagcc ccgccagacc
    25561 taccgccata ccggctcttg ccagctcagc cacctgagca cggcgaccgc cggaagctgc
    25621 gccaaatgga tcgcgacatt gcagtgcgtg ggtaacgtcg aaaatcaccg gcgaattacc
    25681 agacactttc ttcatgatgc tgaagcccag catatcaaca accaggttgt catagccgaa
    25741 gttagcaccg cgatcgcaaa gaatcacttt ttcgttgccg ccttctttga atttatcaac
    25801 gatattaccc atctgtcccg ggctgacaaa ctgcggtttc ttgacgttaa ttaccgcacc
    25861 ggttttcgcc atggcttcaa ccaggtcagt ctggcgagca agaaacgccg gcaactgaat
    25921 cacatctaca acatcagcaa cgggctgtgc ctggcttggt tcgtgaacgt cggtgataat
    25981 tttcacgcca aaagtctgct tcaactcctg gaagattttc atcccttctt ccaggcccgg
    26041 tccacgataa gagtggatgg aggagcggtt ggctttgtca aaagaggctt tgaacacgta
    26101 agggataccc agtttctggg tcacggttac gtagtgctcg caaatgcgca tcgccagatc
    26161 gcgagattcc agcacgttca taccgccaaa cagtacgaac ggcaggtcat ttgctacgtt
    26221 gatgtcgcca atgctaacca ctttttgttt cataggatcg ccttaatctt gagtaaaatg
    26281 tcgattaatt aatgcagcac aatatgttta tgcgcgatgt tatttatttg cgcacggatc
    26341 atttcgctga tcgggtcttc cggacactgt tcaacgaaat aacttaaatc gttcaacgca
    26401 acgtgttcgc aatccagttg cgcataaatc aacccgcgat cgcgaatttc atagggatct
    26461 tccgggttga attgtaataa agcttcgctg gtgcgtaacg ccagctccat ctgattttct
    26521 tccatcaacg aagctttgag tgtatccagc aatttgcgga ttacctcaat gttatcagct
    26581 tcatcaaggt cttcataaaa cagttccgcc gacgggctga tatttccctt taaccacaca
    26641 tccagcatat gttcgcttaa cgattcaccg ttaaaaggat taatcagcca aatttcgcca
    26701 tccggacatt caatgcgcaa tatcagctgc gtagggaaaa tcaccggcag cagcggcaaa
    26761 tcgagacgat tcgcgaccca taataaaacc gcacctaatg ataccgcact gccctgtcga
    26821 ttctttaaca cctggtccag ccataatgca tcggaaagac gataaacgcc gcgtgaggct
    26881 ttaaaccccc attcgccgta aaacagcgcg attaattttt ctagctgctc ttctaatggc
    26941 agaagctggc taatttcttc cttcgccaga ctaacgagac gctccagttc gtcgtaaaca
    27001 tcttgcgagg gaaaatcgcg gcggattgct tcgcaagcca ggatcatgcc ttcgcacaat
    27061 ggcgctttat taaattcgaa atcagctaac gatctcatga cttaccccag taacggtact
    27121 tttgtggtgg cgagtttaat gatgatgtac agcaccacca acgccagcgg gaaggcaata
    27181 atgcgcgcct gctgactgtg catacgacga taatcgagtg caataaaacc caaaacgata
    27241 taaatgataa ctccaaacag cttttcagtc agccactgtg cctgcccgga aaatggctgg
    27301 atgtgcgctt tagccatcaa cgcaatgccg cttaacaata acagcgtatc gacaaccggc
    27361 ggcacaatgc gcgtccagcg agcaaatgcc tgtggatgct tctgataacg tagccagaaa
    27421 cgtaaggtca atagcccaac agaaagcgcg atactaatga gatgaacgct aagcaacgta
    27481 gaaaaacttg tcattgataa tagcggccga gcgttacgcg ctcgttatcc ccatagtcac
    27541 ggcaggtttc gacgtcatga taccccgcga agataaatgc ctgtcgcacc gcttcaccct
    27601 gctgccagcc atgctccaga agcagaaagc cgccggatac cagcgcgtta cgcgactgtt
    27661 cgatgatatg cacgatgtct gccattccac tgtctgccgc aaccagcgca gtgagcggct
    27721 caaagcggac atcgccttgt tgaagatgtg ggtcctgctc gtcaatatac ggcggattgc
    27781 tgacaatcat cgcaaactgc tgcccggcta gcgcgctaaa ccagtcgctt tgcagaatgt
    27841 ggatattttt gatagccaga tgctgggcat tacgttgcgc cagagagaca gcatctggca
    27901 tacgatctac agcggtaatt tcgcagtccg gacgctcgct ggccagcgcc agcgcaatcg
    27961 ccccggtacc cgtcccgaga tcgagaatac ggcaaggttg ttcaggcaac cgcgccagtg
    28021 cctgctccac cagacactcc gtatccgggc gcggaattaa ggtcgctggc gaaacaaata
    28081 acggcaacga ccagaattct cgcaccccgg ttaaatgagc aatgggttca ccatcgcgac
    28141 gacgcgtcag tagcgcatca agttgctgac attgttcgtc agtcagctgc gtttcaccaa
    28201 aggcgagaat aaaagtacgc cctctgccgg taacatgctc cagcaggatt tcagcatcac
    28261 gccgcgggct ttcgctcgcc tgaagttggc ttattgcttc acgtaaccag tgttgatatt
    28321 ccattattcc tgctcggaca acgccgccag ttggtcggcc tgatgttcct ggataatcgg
    28381 ttcaatcagc atatccagct taccttccat cacttcatcc aggcggtaga gcgtcaggtt
    28441 gatgcggtga tcggtaacgc gcccctgcgg gaagttgtaa gtacggttac ggtcgctgcg
    28501 atcgccactc cccagcaggt tacgacgggt agacgcttcg gcctgttgcc gttttgccat
    28561 ttcagcagcg tggatgcgag caccgagtac agaaagtgct ttagctttgt ttttatgttg
    28621 tgaacgttcg tcctgacatt caacaacaat cccggtcggc aagtgagtaa tacgaattgc
    28681 cgaatcggtg gtgttaacgt gctgaccacc cgcccctgac gagcggaaag tatcaatgcg
    28741 taaatctgct gggttgatgt ccggcagttc tgcatcaggc agttctggca ttaccgcaac
    28801 ggtacaagca gaagtatgaa tacgaccctg cgattccgta gcaggaacac gctgcacgcg
    28861 atgaccgcca gattcgaatt tcaaacgacc atatacacca tcaccgctaa ttttggcgat
    28921 gatctcttta taaccaccat gttcaccctc gctggcgctc atgatttcta cacgccagcg
    28981 gcgggcttcg gcgtaacggc tgtacatacg gaacagatcg ccagcgaaca gcgccgcttc
    29041 gtcgccgccg gttccggcac ggacttcgag gaaggcgtta cgttcgtcat caggatcttt
    29101 tggtagtaac agaacctgta attgctgttc cagttgctcg cttttttctt tagcttcgcg
    29161 cagttcatcc tgcgccatct cacgcatttc aggatcgtcg agcatcatct gtgcggtttc
    29221 gatatcttcc tgaacctgtt gccagtcggt aaaacagcgc gaaacatcac ttaactgcgc
    29281 atattcacga gataatgcgc gaaaacgttc ctggtcagcg atggtttgcg catcacccag
    29341 cagcgcctga acttcttcat ggcgttcatg cagggcttcc agtttggcaa cgatagaagg
    29401 cttcataggc gtaaatgcac cctgtaaaaa aagaaaatga tgtactgcta ctccagcccg
    29461 aggctgtcgc gcagaatatt caggcgttcg ttatccccgt cacgggcggc ctgttgaagt
    29521 gattttgttg gtgcatggat caggcggtta gtcagtttcc atgccagatc ctgcataatg
    29581 gtttgcgcat cgccgccctg ttcaagggcc gctaacgctt tagcagttaa ctcatcacga
    29641 acctgctctg cctggctacg ataatcgcga atagtttcgc tggcgctttg tgctcgaagc
    29701 catgccataa attcactggc ctcctgggcg acgatagttt ccgcctccac agcagcagct
    29761 ttccgttgcg ccaggttgtg cgaaataatg ctttgcagat cgtcaacgct ataaagataa
    29821 gcattcgcca gtttaccaac ttccggctca acatcgcgcg gaacggcaat atccaccagc
    29881 agcattggtt gattgcgacg gctttttaat gcgcgctcta ccatgccttt accgataatc
    29941 ggcaacgggc tggcggtgga actgatgatg atatcggctt cgcgcagacg ttcgtcgata
    30001 tcgctcaggg caatcacttc cgcgccaacc tcatctgcca gaatttgggc acgttcgcga
    30061 gtgcgattgg cgataatcat cttctgtact ttgtgttcgc gcagatgacg cgccaccagc
    30121 tcgatggttt cgcccgcgcc aaccagcagt accgtcaccg tagagagcga ttcaaagatc
    30181 tgccgcgcca gcgtacaagc cgcaaaagcg acagacacag cactggcacc gatatctgtt
    30241 tcagtgcgaa cgcgtttcgc cacagagaaa gatttctgaa acatgcgttc cagttcgctg
    30301 gccttcatat gacctttttg cgaatcggca aacgcttttt taacctgacc gaggatctgc
    30361 ggctcgccaa ggacgagaga atcaagccca ctggcaacgc gcattaaatg actgaccgca
    30421 tcgttatcct gatgccagta gaggctatta cgcagatcgt cttcgttgag attatggtaa
    30481 tcacacaacc agcgaatgag cgcctcttgc aggtcatccc gttcttcaac gctgagataa
    30541 agttctgtgc ggttacacgt cgacagcacc acaccgccct gcaccatcgg ctgcgcgagc
    30601 agactgtcaa gcgcctgatc gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat
    30661 acaggtgccg ttttatggtt gataccgagt gctaaaaggg tcatgtctgc gggaaataat
    30721 accaacgttg atagggttag tctgcttgca tcatacagga tgcgtaagat caataaaaga
    30781 gagcgccccc ttttggagta attgccgaaa gccgttagat tctggcaatt aagacaacgt
    30841 gaacatagac gatggctaac ggtaacgcta gcattaaggg ttataactgc aacgtatctc
    30901 aaggacttgt cattattatg cccctgcccg attttcgtct tatccgcctg ctaccgctgg
    30961 ctgctcttgt gctcactgcc tgttccgtta ccacgcccaa aggtcctggc aaaagcccgg
    31021 attcgccaca atggcgtcag catcagcaag acgtgcgcaa tcttaatcag tatcaaactc
    31081 gcggcgcgtt cgcttatatt tctgaccaac aaaaagtgta cgcccgtttc ttctggcaac
    31141 aaactggcca ggatcgctac cgtctgctgc tgactaaccc attgggcagc acggaactgg
    31201 agctgaatgc tcaaccgggt aacgtgcagt tagtcgacaa taaaggtcag cgttatacct
    31261 ccgatgacgc cgaagagatg attggcaaat tgaccggaat gccaattccg ctcaacagct
    31321 tgcgccagtg gattttaggt ttaccgggtg atgcgactga ctacaaactt gacgaccagt
    31381 accgcctgag cgaaatcacc tacagccaga atggcaaaaa ctggaaagtt gtttacggtg
    31441 gttatgacac aaaaactcaa cctgcgatgc cagccaatat ggaactcacc gacggtggtc
    31501 aacgcatcaa gttaaaaatg gataactgga tagtgaaata atgcggacac agtggccctc
    31561 tccggcaaaa cttaatctgt ttttatacat taccggtcag cgtgcggatg gttaccacac
    31621 gctgcaaacg ctgtttcagt ttcttgatta cggcgacatc atcagcattg agcttcgtga
    31681 cgatggggat attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag ataacctgat
    31741 cgttcgcgca gcgcggttgt tgatgaaaac tgcggcagac agcgggcgtc tttcgatggg
    31801 aagcggtgcg aatatcagca ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg
    31861 ttcatccaat gccgcgacgg ttctggtggc gttaaatcat ctctggcaat gcggactaag
    31921 catggatgaa ctggcggaaa tggggctgac gctgggcgca gatgttcctg tctttgttcg
    31981 tggtcatgcc gcgtttgccg aaggcgttgg tgaaatacta atgccggtgg atccgccaga
    32041 gaagtggtat ctggtggcgc accctggtgt aagtattcca acaccggtga tttttaaaga
    32101 tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt
    32161 cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc
    32221 ctggctgtta gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg tctttgctga
    32281 atttgataca gagtctgaag cccgccaggt gctagagcaa gccccggaat ggctcaatgg
    32341 ctttgtggcg aaaggcgtta atctttcccc attgcacaga gccatgcttt aagccgggca
    32401 agctgagttt cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct ctttaataca
    32461 ccgcctggaa agaatcatgc ctggcccgca cagttttcgg cagattcttt ccaccaatgg
    32521 acgcatgcct gaggttcttc tcgtgcctga tatgaagctt tttgctggta acgccacccc
    32581 ggaactagca caacgtattg ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg
    32641 tcgctttagc gacggcgaag tcagtgtaca aattaatgaa aatgtacgcg gtggtgatat
    32701 tttcatcatc cagtccactt gtgcccctac taacgacaac ctgatggaat tagtcgttat
    32761 ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc cctactttgg
    32821 ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt
    32881 tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg acagtggatc tgcacgctga
    32941 acagattcag ggtttcttcg acgttccggt tgataacgta tttggtagcc cgatcctgct
    33001 ggaagacatg ctgcagctga atctggataa cccaattgtg gtttctccgg acatcggcgg
    33061 cgttgtgcgt gcccgcgcta tcgctaagct gctgaacgat accgatatgg caatcatcga
    33121 caaacgtcgt ccgcgtgcga acgtttccca ggtgatgcat atcatcggtg acgttgcagg
    33181 tcgtgactgc gtactggtcg atgatatgat cgacactggc ggtacgctgt gtaaagctgc
    33241 tgaagctctg aaagaacgtg gtgctaaacg tgtatttgcg tacgcgactc acccgatctt
    33301 ctctggcaac gcggcgaaca acctgcgtaa ctctgtaatt gatgaagtcg ttgtctgcga
    33361 taccattccg ctgagcgatg aaatcaaatc actgccgaac gtgcgtactc tgaccctgtc
    33421 aggtatgctg gccgaagcga ttcgtcgtat cagcaacgaa gaatcgatct ctgccatgtt
    33481 cgaacactaa tcgaacccgg ctcaaagacc cgctgcggcg ggtttttttg tctgtaatat
    33541 ccatttgtat gacctatgcc tccttcacct gccatttagt tgacagatga tgcgctcacg
    33601 gatgaaacat tattgtgaac aaaatatttt cctcacatgt gatgcctttc cgcgctctga
    33661 tcgacgcttg ctggaaagaa aaatatactg ccgcacggtt tacccgtgac ctgattgccg
    33721 ggataaccgt cgggattatt gctatcccgc tggcgatggc gttggctatt ggtagtggtg
    33781 tggcccccca gtacggttta tataccgcag ctgttgcggg gattgtcatt gctctgacgg
    33841 gggggtcacg ctttagcgtt tccggtccga ctgcggcatt tgtggtaatt ctctatcccg
    33901 tttcgcaaca gtttggactg gcaggactgc tggttgcgac cttgctgtcg ggaatctttt
    33961 tgattctgat ggggctggca cgctttggtc gcctgattga gtatattccg gtttccgtca
    34021 ccttaggttt cacctcgggt atcgggatca ccatcggtac catgcagatt aaagattttc
    34081 tcggtctgca aatggcccat gtcccggaac attatctaca aaaagtcggc gcattattta
    34141 tggcgctgcc gaccattaat gtgggtgatg cagccattgg cattgtgacg cttggcattc
    34201 tggttttctg gccgcgtctg ggcatacgtt tacccggcca ccttccggcc ttgctggctg
    34261 gttgcgcggt gatggggatt gttaacctgc tcggcggaca tgttgctacc atcggttcgc
    34321 aattccacta cgtcctggcc gatggttctc agggtaacgg tattccgcaa ctgctgccgc
    34381 aactggtgct gccgtgggat ctgcctaatt cagaattcac gctaacctgg gattctattc
    34441 gcaccctgct gcctgcggca ttctcaatgg caatgctcgg cgcaatcgaa tctctgctct
    34501 gcgccgtggt gctggatggt atgaccggga cgaaacacaa ggcgaacagc gaactggttg
    34561 gacagggact ggggaatatt atcgctccgt tctttggtgg tattaccgct acagctgcca
    34621 tcgcgcgttc tgccgctaac gtccgtgccg gggcaacttc ccctatctcg gcggtgatcc
    34681 actctattct ggttattctt gccctgctgg tactggcacc gctgctctcc tggctgccgc
    34741 tttccgccat ggcagccctg ctgttgatgg tggcgtggaa catgagtgaa gcgcacaaag
    34801 tggtcgactt gctgcgtcat gcaccgaaag atgacatcat cgtcatgctg ctgtgcatgt
    34861 cgctgaccgt gctgttcgat atggttattg ccatcagcgt ggggatcgtg ctggcatcgc
    34921 tgctgtttat gcgtcgtatc gcacgtatga ctcgcctggc accggtagtc gtagacgttc
    34981 cagacgatgt cctggttctg cgcgttattg gcccgctgtt ttttgctgcc gctgaaggct
    35041 tattcacgga cctggagtca cgtcttgaag gcaaacggat tgtgattctg aagtgggatg
    35101 ccgttccggt acttgatgct ggtggtcttg atgcgttcca gcgttttgtg aagcgtctgc
    35161 ccgaaggatg tgaactgcgc gtgtgcaacc tggaattcca gccactgcgc actatggctc
    35221 gcgcaggcat tcaaccgatc ccgggacgcc tcgcattctt cccgaatcgt cgcgcggcga
    35281 tggcggattt ataagtacga gattgaccag tcagcgcaaa ctgactggtc agcaaactgc
    35341 attatttgtt agctatgacg gcgattatcg ctacggcggc aacgtttgtc atagtggcga
    35401 acccaccagt aacggtcgca cacctgttca tgcccgccaa cacgggcacc tgccagccag
    35461 agaatcgcac cgacgaaaat acttagcaca gcaccatgtg cgaaatactg gggcatatta
    35521 aactgtggta actggttgag gattgaatac cccacgccga ccaccattac caccagaccc
    35581 aaccccatga gcacgttacc gagtaacgaa gcgtttttgc gtttcatatg tcacctccgg
    35641 aactttctgg gttgtaacag ggaatacccc tcttccttat gcgtaaagta tagacaacac
    35701 cctgtgggtt atgtgcgggt gtgatcacaa ttacaaccct tacttcaaca aaactttaca
    35761 aataaacgcc tgaacacctt gactttctaa tagtccactc aggcaataat tgcattatct
    35821 acatttgata cgcagttttt tgtcaagcgg ggccgcaacc agtaaactac gcgccagtta
    35881 tgtacacact caggacaaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc
    35941 ggtgctgaat acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca
    36001 gagcgattgc gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc
    36061 acccttggag gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc
    36121 aaagccgttg cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc
    36181 cacgacgaac tggatctgcc tcctggcgtc gccaaattta agttgggtgg cggtcatggt
    36241 ggtcacaatg gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt
    36301 ttacgcatcg gaatcggtca tccgggcgat aaagataaag ttgtcggttt tgtgttaggc
    36361 aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt
    36421 actgaaatgt ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa
    36481 gcgcaataag tcgttgtctg cggcattttt gccgagtgcc gtgtataata ggcaaagtta
    36541 tttccatttc tgcaatctgt tagcaataac aggttgatta ttaagatatt aaggtgattt
    36601 aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt
    36661 tcaacgcgct gaccaaagcc ggtattgaag cggccaactt tccattctgc accatcgagc
    36721 cgaacactgg cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa
    36781 aaccgcagcg tacacttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag
    36841 gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag
    36901 cgatcggtca cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag
    36961 ttaacccggc ggatgatatt gaagttatca acaccgaact ggcgctggcg gatctcgaca
    37021 cctgtgaacg tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aatgacgcga
    37081 aagccgagct ggcggtactg gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc
    37141 gtgcgctgga tttaagcgct gaagagaaag cggctgttcg ttacctgagc ttcctgacgc
    37201 taaaaccaac aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccgtatc
    37261 ttgaccaggt gcgtgaaatc gcagcgaaag aaggttctgt tgtagttccg gtttgtgctg
    37321 ctgttgaagc agacattgcc gaactggacg acgaagagcg tgacgagttt atgcaggagc
    37381 ttgggctgga agagccgggc ctgaaccgtg tgatccgtgc cggttataag ctgctgaacc
    37441 tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggtg
    37501 caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg
    37561 cacaaaccat ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaggaag
    37621 caggcaaaat gcgtgcagaa ggtaaagact acatcgttaa agatggcgat gtgatgaact
    37681 tcctgttcaa cgtctaattc cgcgtaatat cctggcggtt aataacgagc cattatgtga
    37741 gttgcacgta atggcttttt tacgcctgct acgcgtcttc tttcaattct gagttaccaa
    37801 aactcataat cagactcctg acaatattaa caatgcagct tttatgtgat aacaatcaca
    37861 gtgcattaac cgttcaagag tttgtcatga aaaaaacatt taccttcagc ctgttagcaa
    37921 ttgtctttgc aagcctgttg agtgcttgtg ttccccacca tcatcaccgc cacagcgatg
    37981 gccctcgtgg tcctgcgccc tcaggtaagc caatgccacc ttcaaatggt ggaccaaatc
    38041 acggcagtca tggtcgctat taattagcaa ttcatagcgg gaaagtacat actctcccgc
    38101 tatccacggc gcttaaactg ccctgcatca atgccatgtt gcttcatttt ccgccacagc
    38161 gtagtgcggc cgatcccaag taaagccgac atttcctgaa tgcgaccgcc tgtgacctgg
    38221 gctgcgttaa taattgcctc tttttcaact tccgcaaatg acagactggt ggaaaggcgg
    38281 gtggcgctga catcatctgt cgcctgctcg gtaaacagat gttccggcaa atcactgacg
    38341 cgaatgcgcc cattatcact actcagagca agattctcga tgacgctgta aagttcaaaa
    38401 tcgttgccag gccatgcaca ggaaaccagg cgagcgaggg catcgtcatc aattttcagc
    38461 cgcgtagaga agcgtttttc aaggctgcgt aatttgttat tcaccagcgc cggaatgctg
    38521 ccacgccgca tacgcagtgg cgggatggta atttcaaatg catgcagcgc gtaatacagc
    38581 tggcgactaa aacgattttg ttccaccagc attgcgaggt ccgcggtcgt tgtggcaatc
    38641 actttgacat caatgggtat taaacgccgc gcatccagtc gcgtgataac cccctgcttg
    38701 ataacctgaa gcaaggcaga ctgtaactcc accgccagat attcaatctt ttcaagaaac
    38761 agcgtgccgc cgtgcgccag ttccagccga ctcagacggc cattttcact atccgtgcga
    38821 tcgccaccaa taaattcttc cgccagcgcc gcatcaccat ataactcaca attgacggcg
    38881 atataaggcc ccgctgcacg ctcgctttca ttatgaattg cctgacttag cagtgccttg
    38941 cccacgccct cttctccaca aagcaggaca ggaaagctac tgcgtgccgc ctggcgacca
    39001 aaatgaatca agcggcgggt ttgcggatcg tcctgtggca tatgagcgaa ggtatggctg
    39061 acttttccta attgactggt cattaactgc cgcatctgtt ccacaggatg gagcaataaa
    39121 ataaagctgg ttccctgcgt ttctattatc ggtttaaggg ttatcaccgc atcaataaac
    39181 tgatgctgac tttcaaaggt ggcttctacg tgtttgagcg gatgtgcctg ttttattgct
    39241 tgttgcaata cggcgggtaa cgttaagagt tcagtgattg cccttccctg acttgccgtc
    39301 gcgtcaaggc gcaagactcg cgccgcctgg gcgttgataa attgcaaatt accctgctcg
    39361 tcccagctaa tcacgccatc atccatactt tctaacaggg cattaagttg atttaaatga
    39421 cggttagttt cagcgagcaa actgtccgtc agcagtaaat ttccgacctc gcgtgcgatt
    39481 gccaacgtca acggcaaatc agctgcggta gtttgctcaa ccggacacgc cagcgctatt
    39541 gttcccgtca atcggccctt gctgtcaaac aacggcgttg cgcaaaaggc ccagttccag
    39601 agtgcctgtt tgaaatgttg atcggccatc gttttcacgg cctgaccaga gatagccgct
    39661 aacgatagcg cacaagtacc aataattccc tcggcgcaat acgtgccgtc attgaatccc
    39721 agtacactta gctgctgcaa ggtttgcgga tcgccattac ggctgagaat gcaggcggtt
    39781 tcatcgagga taagcaacgc acactctcgc ggtgccatat attcccaggc atcttccagc
    39841 gcagcctgcc cgagcgtcag catcgctttc ttacgccgat aaatagaagc aaatgtcacg
    39901 ccctgggcct ggtgtggtac gttccatgtc tcccgtttca tcagcttatt gcatctctcc
    39961 caggaggttg caattaaggg agatatgccc cgaccatcgt tgttaaaagc gccactcata
    40021 tcccgcatcc cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt aatacacgta
    40081 aaagtttcaa tatggaacat tcattaaagc atatgtttca ctatgaaaca taatttgagc
    40141 taattttctt ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt ggtcgccgtg
    40201 tcgttgaaca tcatccatgc cctaccgtaa ttgctggagc aaaataatga aaaaattgat
    40261 caatgatgtg caagacgtac tggacgaaca actggcagga ctagcgaaag cgcatccatc
    40321 gctgacactg catcaggatc cggtgtatgt cacccgagct gatgcccccg ttgcaggaaa
    40381 agtcgccctg ctgtcgggtg gcggcagcgg acacgagccg atgcattgtg gttatatcgg
    40441 tcaggggatg ctttctgggg cctgtccggg cgaaattttc acctcaccga cgcccgataa
    40501 aatctttgaa tgcgccatgc aaattgatgg cggcgaaggt gtactgttga ttatcaaaaa
    40561 ttacaccggc gatattctta acttcgaaac agcaaccgag ttactgcacg atagcggcgt
    40621 aaaagtgacc actgtggtca ttgatgacga cgttgcagta aaagacagtc tttataccgc
    40681 agggcggcgc ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggtg cagcggcgga
    40741 gcgtggcgac tcactggacg cctgtgcgga actggggcgt aagttgaata atcaaggcca
    40801 ttcaataggt atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac cttcttttac
    40861 cctggcggat aatgagatgg agtttggcgt cggcattcat ggtgagccag gtattgaccg
    40921 ccgccccttc tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc tgctggaaaa
    40981 tggctcatac catcgcactt tgcgtttctg ggattatcaa caaggcagct ggcaggaaga
    41041 accacaaacc aaacaaccgc tccagtctgg cgatcgggtg attgcgctgg ttaacaatct
    41101 tggcgcaact ccgctttctg agctgtacgg cgtctataac cgcctgacca cacgttgcca
    41161 gcaagcggga ttgactatcg aacgtaattt aattggcgcg tactgcacct cactggatat
    41221 gaccggtttc tcaatcacct tactgaaagt tgatgacgaa acgctggcac tctgggacgc
    41281 ccctgtccac accccggccc ttaactgggg taaataagga gaaagcaatg tcactgagca
    41341 gaactcaaat tgttaactgg ctcactcgtt gtggcgatat tttcagcacc gagagcgagt
    41401 atcttactgg actggatcgc gagattggcg acgctgacca cgggctaaat atgaatcgag
    41461 gctttagcaa agtggtggaa aaactccctg ctatcgcaga taaagatatc ggtttcattc
    41521 tcaagaatac cggcatgacg ctactttcca gcgtcggagg tgccagtggt ccgctgttcg
    41581 gtaccttctt tatccgcgcc gcacagacaa cccaggcacg gcaaagcctg acgctggaag
    41641 agctttatca gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg aaagccgaac
    41701 ctggcgataa aaccatgtgt gatgtgtggg tgccggtggt ggaatcgtta cgtcagtcct
    41761 gcgagcaaaa tctccctgtt ccggcggcgc tcgacgctgc cagtagcatc gccgaatccg
    41821 ctgcacaaag tacgattacg atgcaagccc gcaaaggccg cgccagttat ctcggtgaac
    41881 gcagtattgg tcaccaggat cccggcgcga cctcggtgat gtttatgatg caaatgttgg
    41941 cgttagccgc aaaagagtaa ggaattggtg atggtaaacc tggtcatagt ttcacatagc
    42001 agccgactgg gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag tgataattgt
    42061 aaaatcgcca ttgccgcggg aattgacgat ccacaaaatc ccattggtac cgatgccatc
    42121 aaagtgatgg aggccatcga atctgttgcc gatgccgacc atgtgctggt catgatggat
    42181 atgggtagcg cattattgag tgctgaaact gcgctggaat tgctggctcc cgagatcgcc
    42241 gcaaaagtac gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc aacggtcagc
    42301 gcggcctcgg gggcggatat cgacaaagtg atctttgacg ccatgcatgc gctggaagcc
    42361 aaacgtgaac aactgggttt accgtcctcc gacactgaaa tctctgacac atgtcctccg
    42421 tacgatgaag aagcccgttc tctgtcggtg gtcataaaaa accgtaacgg cctgcatgta
    42481 cgtccggcct cccggctggt ttatacctta tcgacattta atgccgatat gttgctggag
    42541 aaaaacggca aatgcgttac accggagagt attaaccaga ttgcgttact acaagttcgc
    42601 tataacgata cgctgcgcct gattgcgaaa ggaccagaag ctgaagaggc actgatcgct
    42661 ttccgtcagc tggctgaaga taactttggt gaaacggagg aagttgctcc acctactctg
    42721 cgtcccgttc cgcctgtttc gggtaaagcc ttttattatc aacctgtttt atgtacggta
    42781 caggcaaaat caaccctgac cgtggaagaa gaacaagaac gattacgcca ggccattgac
    42841 ttcacgttat tagatctgat gacgttaaca gcgaaagcag aaaccagcgg gcttgacgat
    42901 attgccgcaa tcttttctgg tcaccataca ctgttagatg atccggaact gcaggcggcg
    42961 gcaagcgaac tccttcagca tgaacattgc acggcggaat atgcctggca gcaagttctt
    43021 aaagaactta gccagcaata ccaacagctg gatgatgaat atctacaggc tcgatatatc
    43081 gatgtggacg atcttctgca tcgcaccctg gtccacctga cccaaacgaa agaagaactc
    43141 ccgcagttta actcgccaac tattctactg gcggagaaca tttatccctc cacagtactg
    43201 caactggatc cagcggttgt aaaaggtatc tgccttagcg ccggaagtcc gctatcccac
    43261 agtgccctaa tcgcccgtga actgggaatt ggctggattt gccagcaggg tgagaaactg
    43321 tatgcgatac aaccagaaga aacgctaacg ctggacgtta aaacgcaacg tttcaaccgt
    43381 cagggttaaa ccaccgttcg gatggcatca ttctgatgtc atccggcaat ttactctgca
    43441 ctgatgtttt taactacctg ttatattttt caccaaatta tgatgatttt tcttcgctaa
    43501 tcgcaaaccc aacgtcgctg gctgactaat aaacagatgt tattttatca ctctatttcg
    43561 ccgttattta tccctttaat tcccttttct aaaatgtctg tcagttcgca gaatgtgatt
    43621 tcgatcatgc agctagtgcg atcctgaact aaggttttct gatacttgaa taccgtttta
    43681 ttcggtttcg ccaaaggaga atgactgatg aaatcccccg caccttctcg cccgcaaaaa
    43741 atggcgttaa ttccagcctg catctttttg tgtttcgctg cgctatcggt gcaggcagaa
    43801 gaaacatcgg taacaccaca accgcctgat attttattag ggccgctgtt taatgatgtg
    43861 caaaacgcca aactttttcc ggaccaaaaa acctttgccg atgccgtgcc gaacagcgat
    43921 ccgctgatga tccttgctga ttatcggatg cagcaacacc agagcggatt tgatctacgc
    43981 catttcgtta acgtcaattt caccctgccg aaagagggcg agaaatatgt tccgccagag
    44041 gggcaatcac tgcgcgaaca tattgacgga ctttggccgg tattaacgcg ttctatcgaa
    44101 aacaccgaaa aatgggattc tctattaccg ctgccaaaac cttatgtcgt gccaggcgga
    44161 cgttttcgcg aggtatatta ctgggacagt tacttcacca tgttaggact tgccgaaagc
    44221 ggtcactggg ataaagtcgc ggatatggtg gccaattttg ctcatgaaat agacacttac
    44281 ggtcatattc ccaacggcaa ccgcagttac tatttaagcc gctcgcaacc gcccttcttt
    44341 gccctgatgg tagagttact ggcgcagcat gaaggcgacg ccgcgttgaa acaatacctg
    44401 ccgcaaatgc aaaaagaata tgcttactgg atggacggtg ttgaaaacct gcaagccgga
    44461 caacaggaaa aacgcgttgt aaaacttcag gatggtaccc ttctcaaccg ctactgggac
    44521 gatcgcgata cgccacgacc agagtcatgg gtggaagata ttgccactgc caaaagcaat
    44581 ccgaatcgac ctgccactga aatttaccgc gacctgcgct ctgccgctgc gtctggctgg
    44641 gatttcagct cgcgctggat ggacaatccg cagcagttaa acaccttacg caccaccagc
    44701 atcgtaccgg tcgatctgaa cagcttgatg ttcaaaatgg aaaaaatcct ctcccgcgcc
    44761 agcaaagctg ccggagataa cgcgatggca aaccagtacg aaacgctggc aaatgcccgt
    44821 caaaaaggga tcgaaaaata cctgtggaac gatcaacaag gctggtatgc cgattacgac
    44881 ctgaaaagtc ataaagtgcg caatcagtta accgcggccg ccctgttccc gctgtacgtc
    44941 aatgcggcag cgaaagatcg cgccaacaaa atggcgacgg cgacgaaaac acatctgctg
    45001 caaccaggcg gcctgaacac cacgtcggtg aaaagtgggc aacaatggga tgcgccaaac
    45061 ggctgggcac cgttgcagtg ggtcgcgaca gaaggattac aaaactacgg gcaaaaagag
    45121 gtagcgatgg acattagctg gcacttcctg accaatgttc agcacaccta tgaccgggag
    45181 aaaaagctgg tggaaaaata tgatgtcagc accaccggaa cagggggcgg cggtggcgaa
    45241 tatccattac aggatggctt tggctggacc aatggcgtga cgctgaaaat gctggatttg
    45301 atctgcccga aagagcaacc gtgtgacaat gttccggcga cgcgtccact cagtgaatca
    45361 acaacacagc cgctaaaaca aaaagaggcg gaacctacgc cttaacttta ctgtatatcg
    45421 agcaatgcca ttacgtgcat aaaattggca ttgctctttc ttcatcacga aataccaaat
    45481 accactcttt ctacggtcta tgcctttatt ttcttttatt agagaagatt ttaattttat
    45541 tttatcaact tgttaatcat tacacctcta tttatatttt cccccactcc ccattctccc
    45601 tgacaatttt cttgtggaac aaagtgttat ttaagaaaca agcaaatcat tctgtctctt
    45661 tagacaaatt ttgacatcaa acatgatctt tttcgctata tgtgtaagta tatagcggtt
    45721 ttaattatca ggatattact tatgtcacta accagacgga ggtttacaca gattcttgcg
    45781 tcgacattgt tcctgcatca tctgccgtcc tttgcacaat cagtcaaatt ctgggcctca
    45841 cggacgcttc ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc gcccgccgat
    45901 ttactattgc tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc
    45961 cgtcaggcat taattccatt gccagagcac attcgccagt tccccaggct gggacgactc
    46021 gccgggcgcg ccagcacact ctcgctggaa gggcttatgg cgttacatcc cgatttggtc
    46081 gttgattgcg gcaatacgga tgaaacctgg atctcccagg cacggcaggt tagcgaacag
    46141 acacaaatac cctggttatt gcttaacggg aaactggaac aatcagcaga acagttaaca
    46201 acgcttggca aaacgttagg cgaagagcac cgcgccgcag aacaagccaa tctcgccagc
    46261 cgcttcgttg gtgaagctca ggcattcgcc acctcacccg ccgctaatct cagcttttat
    46321 gctgcgcgcg gtcctcgagg gctggaaacg ggcttacagg gttcgttgca taccgaggcg
    46381 gcggaattat taggtttgca caacgtcgcg caaatagccg atcgccacgg tctgacacag
    46441 gtttccatgg aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt
    46501 actgcagatt ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt tgcggaacaa
    46561 cgcatcttat ttttaagtgg cctgcccttt ggctggctgg atgccccgcc gggaatcaac
    46621 cgtcttctgg gattacgcag acttcacgcc tggctggatc ccgccatcaa tcgccagttt
    46681 aaaagtgaca tacagcatta cgcccaactg ttctggcatt gttcactcag tgacgccgac
    46741 tatcaaaaat tggtggcgag ctaatgagaa ccgtaaacgg atgtattttg ctgctagcag
    46801 ctatcagtat cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc
    46861 tggcgctcat tcttcgtcaa gaaaatgttc ctgtgcagga acaaattgtc ttctggcaaa
    46921 tccgcgtgcc gcgtattctt gctgcgctgt ttctgggcgc ggcgctggca ggggccggaa
    46981 ccacctatca gggaatgttg cgtaatccct tagtgtcacc ggatatttta ggcgtctccg
    47041 ccggtgccgg acttggtgcc tgcgcggcaa tcctgtgggg attatccatt gtttacatcc
    47101 agctgtacgc attttgcggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc
    47161 gggtgacccg ccacgatccg atcttaaccc tggtgctggt aggtattgcg ttaggcacgt
    47221 tgtgcggagc ggggatatcg ctaataaaaa cgctggccga tccttacacc cagttaccgt
    47281 caattacctt ctggttgctg ggggggctgt ccaccgtgac cttacgcgat ctctgctatg
    47341 ctgcaccgat cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt
    47401 tactcacgct ttccgacgac gaagcccgtt cgctgggact gaacgtgaca cgtctgcgct
    47461 ttggattgat tgtctgcgcc acactcatca ccgccagcac ggtagcaata gcgggcataa
    47521 ttggttgggt cggactggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc
    47581 agcagctgtt gcccatggca atgtgtactg gcgcgattct gctcctgctt accgatacgc
    47641 tggcacgcag cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggcg
    47701 caccgttttt cctgtttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgtccg
    47761 tcatgcgtct ctgggttact cccgccatcc ggtgctacgc gacgtgtcct tcaccctgtc
    47821 gcaaggaaca atttgttgtc tgttaggagt caatggttgc ggcaaaacca cactcatgcg
    47881 ctccattctt ggcgttatac cgttattaaa aggcgagatc ttactggata gcctccctgt
    47941 gcagacactc agccatcgcc agcgcgcgca ggctattgcc tgggtgccgc aggcgcacga
    48001 cggcattttt gcttttagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg
    48061 cgctttttcc gttcccggta agcaagaacg cctgaaagca acggagcaac tggaaaagct
    48121 gggcattttg catctggcga cacgacgctg gaatacgctt agcggcggtg aacgtcagct
    48181 ggctttaatc gcccgtgcac tcgtccagca accgcgcttg ttattactgg atgagcctgc
    48241 ctccagcctc gatttcggtc atcagatcca acttctggac acgctggcgc agctcaaaaa
    48301 taacggcatg acaatgttaa tgtcgactca tcatccgctc catgcaaacg ccattgccga
    48361 cagcattatc caggtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt
    48421 aacaacgaaa aaactggctg ccctgtaccg ggtttctgcc gaccagattc accatcacct
    48481 ctctgcaatt aagcactaag gaaaaatcat gctcattgac gatattgatt ttgcagacct
    48541 ttaccttcag caactcaggc tggcccatcg cacagaaaaa acgccggatc actgggatca
    48601 acgggcggaa aaaatggcgg aaaattgcgc ctcgcctacg gacagctacc tgcaacaact
    48661 gacctcaaaa attgacctgc aaggcgcgca aaccttattc gatatgggct gcggaccggg
    48721 taccgtatcg ctggcgctgg cggacaaact cacgacgatt tatggcgttg attacagtca
    48781 ggggatgctg aacgttgccg cccgtcgcgc cgccgcgcta aaggcagaca atgtccactg
    48841 gatccagcgc gcctgggaag aggactggag tgacctacca cgctgcgata ttgccgtggc
    48901 ttcccgctcc actctggtgg ctgatatgcg ccaggcaatg agcaaactga ataatcaggc
    48961 acggcttcgc gtctatacga ctcatctggt cagcacctcg ttcgtctctc cagccattca
    49021 gcgggcggct ggtcgggaag tgatcgaatt acccaactat atctttgcac tcaacgtcct
    49081 gtatcagatg ggaatttatg ctcacgttga ttttatccgt ggacaaaatt gccaacagga
    49141 taacagcacc tgggaacgtt ttgagcagaa cgtcagctgg agtctgggcg cgcttaacga
    49201 cgatgagcgt gagcggttat atcgctggta tcaacaacaa gatgctcgtg ccctcgcccc
    49261 tgccagccgc gactgggcgc tcatctggtg ggatagcgta ccgcaggaga cactgcgatg
    49321 attttccttt ctcaggcaca aatcgatgcg ttactgctgg aagatatcca gggcggtgac
    49381 ctgaccacgc gagcattaaa tattggacac cagcatggct atatagagtt ttttctccgt
    49441 cagggcggct gcgtcagcgg aatttctgtc gcgtgtaaga tgttaactac gctgggatta
    49501 actattgatg acgcggtcag cgacggttct caagcgaacg cgggtcagcg gctaatccgt
    49561 gcgcaaggta atgccgcagc acttcatcag ggatggaagg cagtccagaa tgtgctggag
    49621 tggagttgcg gtgtttctga ttatctcgct caaatgctgg cgttacttcg tgaacgttac
    49681 cctgatggca atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcctcg
    49741 caggcaattc tggctgccgg aggactgatt catcgtgccg gatgtgcgga aaccatatta
    49801 ttgtttgcca accaccgcca ttttcttcat gacaatcagg actggtcagg cgcaatcaat
    49861 cagttacgtc gccacgcacc cgagaagaaa atagttgttg aagccgacac gccgaaagag
    49921 gcaatcgccg cgttacgcgc gcaaccagac gtacttcagc tcgacaaatt tagtccgcaa
    49981 caggcaactg aaattgcgca aatagcaccg tcgctggctc cccactgcac gctggctctg
    50041 accggcggaa ttaatctgac aacactcaaa aattacctcg actgcggcat tcgccttttt
    50101 attacctccg cgccttatta cgcggcacct gctgacatta aagtcagtct gcaacccgca
    50161 gcctctattt aattctttgt taaacggaat ttattctatg agactcaaaa aacgttatct
    50221 ctgcacagta ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgacac
    50281 gctgaccgta tggtccagtc cggtatcatc aacgacgacc accgttctcg atcaacccac
    50341 catgaaggcc ctggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt
    50401 gctgcaaaag tcaggtagcc gcaacgaaga acaggtcaaa gttcgtggct ttgatagtcg
    50461 tcaggtgccg gtctatttcg acggtgtgcc catttatgtt ccctatgacg gcaacctcga
    50521 tctggcgcgg attctgacca acaatttggg ggcagttgaa gtttccaaag ggtattcgtc
    50581 gctgcttcag gggcctaatc agatgggcgg agccattaat atcaccaccc agaagccaac
    50641 aaaacctctg gaagcaaatc tgggatatcg ccagggatgg agccgtagcc aggacaatgc
    50701 ctacgatatg catgcttcat ttgccgccag cagcgatctg gggtatttgc aagtcagcgg
    50761 tagccagcta aagcaggatt ttctcggcct gccgcatggt gtaaataatg atattgcagg
    50821 caaacacggc aagatgatta attcatcggc tgatgataaa cgcggcattg tgaagctcgg
    50881 ttttacacca cgcgaaaacg atgaatacac attgacttac attaagcagg atggtgaaaa
    50941 agataacccg ccatacagtg gaaatagtgg tcagaaatca cgctactggc agtggccaga
    51001 gtatgacaaa gaaagttttt attatcaggg aacgacccaa ctaaacgatc gttttaccct
    51061 gaaaagtcgg ctgtatcacg acacctttga aaatacgcta atgatgtaca actcgctggc
    51121 tgatttgaaa aataaaaaag gcagctacag ccattattcc gattacagcg acggtgccgg
    51181 gttacaactg gcagccgatg tgcgtgaaaa cgatctgctg tcgtttgccg ttaactggaa
    51241 agatgacgta caccgggaaa aaggtgcgcc gcacgccgct tacgatcgct atgaagatcg
    51301 tacctggtcg ctcgccagtg aatatcaatg ggctgctgcc gataatgtcg atgtcgtggc
    51361 tggaatcagc tatgactggc gcgatagcgt agaagcgaaa aaacatgaga aagatggcag
    51421 tatcacccat tatgacgaca acaatcagtc agcttttaac tggcaggtga tggggaaata
    51481 ccactttgcc aatgaagaca cgctggcgct ttcgtactat gaccgcacac gctttccgac
    51541 gctgaaagaa cgctatacca cgtccaaacc tgcgtataac cagatagcga ttgttaaccc
    51601 gcagctcaag ccggaacgcg cgcgcggggt ggatttaacc tggaatggtg ccttcacgca
    51661 cgactgggga tttgaggtca gcgtttacta taaccgggtg agtgatgcca tcctctcgca
    51721 caatatcgat gccgatacca ttcaaaatca gaacagcggc acggtggatt acagcggtct
    51781 ggatgccggt attaagggga aaatcagcaa tatactggat gtaggattga gctacgccct
    51841 gatccacgct gatgccaaac gcaaagacat cggcaagata accgatctgc caacgcagac
    51901 aatgaccgca tggatgactc tcaaaccgtg ggagccgtta agcgtaacgc tgtcggaaga
    51961 ggcgcgttcc tccagctaca gcaacagtga cggttcacaa aaagccgccg gttttgcggt
    52021 gacccacatt cgagccgatt acaccttagg tcatggcttc agcgttaatg cgtcggtcaa
    52081 taacctgttt gataccaaat atgcctacag tgaagggttt attgaagaag gtcgcaattt
    52141 ctgggctggt atcgagtata cgttctgatg tgaaagcgga tagcggcatc tgtatcgcta
    52201 tccgccttat gaaaatctta ttctcttcgc aggaggcgga atacgcccag caccaggata
    52261 gcgcccacca ccgccaccag gaagctgtgc aaattaaaac cgctgatgga gccgccaatg
    52321 ccaaacatgg tcgccagcca gccgccgacc accgcaccga ctatcccaag aatacaggtc
    52381 aggaaaaatc caccaccatc acgccccggc atgattagct tggcgataat accggctatc
    52441 aggccaaaaa taatccaggc aataattccc atgttcaggc cctcgtttcg atgaatcgga
    52501 cgcaagaaat gcttacgtca acgctctaag tataggcaag ggcgggatcg atgctgggcg
    52561 tatgtcggct gatttaagaa caacatgtaa aaaatattcg cgcatttgta ttaagttttg
    52621 ttaactgcga ccgataaacc aaagacagtt tgtcagtcag gagtttttcc gcgtgagtca
    52681 ttaccatgag cagttcctga aacaaaatcc gttagccgtc ctgggcgtgt tacgcgattt
    52741 gaacaaagcc gcaattcctt tgcgtatcag ctggaatggc gggcagctga tcagcaaaat
    52801 actggcaata actccggata aactggtgct ggatttcggc agccaggccg aagacaacat
    52861 cgccgtgcta aaggcgcagc acattaccat taccgccgaa actcagggtg cgaaagtcga
    52921 gtttactgtt gaacaactac agcagagtga atacttgctg cttccggcat ttattaccgt
    52981 accgcctccc accttatggt ttgtacaacg acgccgatat ttccgcatca ccgccccact
    53041 ccatccgcct tatttttgcc agaccaaact ggcggataac agtacgttac gtttccgcct
    53101 gtatgatttg tcgttaggcg gcatgggcgc attactggaa acagcaaagc ctgccgaatt
    53161 acacgaaggc atgcgcttcg ctcagattga agtcaacatg gggcaatggg gtgtttttca
    53221 ctttgacgcc cagttaatct ccatcagcga gcgcaaagtg attgatggca aaaatgaaac
    53281 catcaccact ccccgtctga gcttccgttt tcttaacgtc agcccgacgg tggagcggca
    53341 attacagcgg attattttct ctctcgagcg agaagcccgg aaaaaagcgg acaatgtgcg
    53401 cgactgattt acatcgcgtc cagtgcctgc tcaagtttgt agatatagcg cggagcctgc
    53461 ggcgcaggat gatttcgcgc tacgtgatcg aggaactcat cagcatccag atcgttgatt
    53521 ttgctgatcg cctttttccg atctgacgag aaagtccgta gcagcgcacc tgccccgtta
    53581 gcgtatgaca ccaccagcgc atattgcagt accttcggat cttcaatgcc tgccagcggg
    53641 ccggtttcca gaatattcag gtaagccgcc cccattgaaa tattacgctc cggatttttc
    53701 agctcgctgg tcgtcggctc accgctccag cccatacgac gatatacatc acgtccggag
    53761 gttgaagctt ttatctgcat caaaccaatg gcattcgatt tactcaccgc gttcggatta
    53821 ccacccgatt cgatagcgat aatcgccgtg atcaattgtg gatcgacgcc ccaggctgca
    53881 ccggctttct ggcttattgg catccactgc atcgcacgtt gcaccggaac tttcgcgttc
    53941 cacggcgggt tcgtatagtc atgctttgat gaacaacccg ctaataacac aatcaaaaag
    54001 gcaaaccatc tcaatttcac ttcttctgtc cttatagcct ggaatcgccg ttgaggtgac
    54061 aacggcacac ctaagcggca tgatatacat ttcgtttctg ttgtcagcaa ggaattgcca
    54121 tgtctctgtt tcacttaatt gccccatcgg gttactgcat taaacagcac gccgcgctgc
    54181 gtggtatcca gcgccttacc gacgcggggc atcaggtcaa taacgtcgag gttattgccc
    54241 gtcgttgtga gcgttttgcg ggtacggaaa cggagcgtct ggaagatctc aattctcttg
    54301 ccagactgac tacccccaac accatcgtgc tgtctgtacg cggcggttac ggtgccagtc
    54361 gtttactggc agatattgac tggcaggcac tggtggctcg ccaacaacat gacccgttac
    54421 tcatttgcgg acatagcgat tttaccgcca ttcagtgcgg tcttctggcg caaggcaatg
    54481 tcatcacctt tagcggcccc atgctggtag caaactttgg cgcggatgaa ctaaatgcct
    54541 ttaccgagca ccacttctgg ctggcgttac gcaataaaac gttcaccatt gaatggcaag
    54601 gggaaggccc gacatgtcag accgaaggca cgctgtgggg aggcaatctt gcgatgctga
    54661 tttcactgat tggtacaccg tggatgccaa aaattgagaa cggtattctg gtgctggaag
    54721 atattaacga gcaccctttc cgcgtcgaac gtatgctgtt gcagctctat catgctggaa
    54781 ttttgccacg tcagaaggcg attattctcg gtagctttag cggcagcacg cctaatgatt
    54841 atgacgcagg ttacaacctg gagtcagtgt acgcgttttt gcgttcccgc ctgtcgattc
    54901 cactcattac cggcctcgat tttggtcatg aacaacgcac ggttacactg ccgttgggcg
    54961 cacatgccat tcttaataac acccaggaag gcacccagtt gacgattagc ggtcatcctg
    55021 ttcttaaaat gtaaaaaaat cacgcttcag ggctaagtaa aacgctgtct ctgccgctaa
    55081 tatggtaggg tttatataag agacagcgta atcaggagta accgaccgtt ggatgccaca
    55141 acaataatta gccttttcat cttaggatcc atccttgtca ccagcagtat attacttagt
    55201 tcattttctt cccgtcttgg cattcccatt ctggttattt ttctggcgat cggcatgctg
    55261 gcgggggttg atggcgtcgg cggtatcccg tttgataatt accccttcgc ctacatggta
    55321 agtaacctgg cgctggcgat tattttgctc gacggcggga tgcgtactca ggccagttcc
    55381 tttcgtgtgg cgttaggacc ggcactgtcg ctggcgacgc tgggcgtgct tatcacctct
    55441 ggtttaaccg gcatgatggc ggcgtggctg tttaatcttg atttgattga aggattatta
    55501 atcggcgcta tcgtcggctc caccgatgct gcagcggtct tttctctgct gggtggtaag
    55561 gggcttaacg aacgtgttgg ctcgacgctg gaaattgaat ccggcagtaa tgatccaatg
    55621 gcggtctttc tgacgattac cctgattgcg atgatccagc aacatgaaag cagtgtcagt
    55681 tggatgttcg tggtcgatat tctgcagcaa tttggcctcg ggattgtcat cgggcttggc
    55741 ggtggttatt tactgctgca aatgattaat cgcatcgccc tgcccaccgg attatatcca
    55801 ttgctggcat taagcggcgg tattttaatt tttgctttaa ccacggcgct ggaaggcagc
    55861 ggtattctgg ctgtttatct gtgcggtttt ctgctgggta atcggccgat tcgcaaccgc
    55921 tacggcatcc tgcaaaattt cgacggcctc gcctggctgg cgcaaatcgc catgttcctg
    55981 gtgctggggc tattggttaa cccaagcgat ctgctgccta ttgccattcc ggcgctcatt
    56041 ttgtccgcat ggatgatttt cttcgcccgt cctctttcgg tatttgccgg attgctaccg
    56101 ttccgtggct tcaatctgcg tgagcgcgtg tttatcagct gggtaggatt acgcggcgcg
    56161 gtgccgatta ttctggctgt gttcccgatg atggcggggc tggagaatgc gcgactgttc
    56221 tttaatgtcg ccttctttgt ggtcctggtt tcactgctat tgcagggaac atcactctcg
    56281 tgggcggcga aaaaagccaa agtggtcgtt ccgccagtgg gacgtccggt gtcacgcgtt
    56341 ggcctggata ttcatccgga aaatccgtgg gagcagtttg tttatcaatt gagtgccgat
    56401 aaatggtgcg tgggcgcggc actgcgtgat ttgcatatgc caaaagagac gcgtattgcg
    56461 gcactgtttc gtgataacca gttgcttcat cccaccggca gcacccgact gcgcgaaggc
    56521 gatgtgttgt gtgtaattgg tcgggaacgc gatctcccgg cgctcggtaa actgttcagc
    56581 cagtcgccgc cggtcgcgct ggatcaacgc ttctttggtg acttcattct cgaagccagc
    56641 gccaaatatg ctgatgtggc gctgatatat ggcctggaag acgggcgaga atatcgcgat
    56701 aagcagcaaa cgctgggtga aatcgtccag cagttgttag gcgcagcacc ggttgtcggt
    56761 gaccaggtag agtttgccgg gatgatctgg acggtggccg agaaagaaga taatgaagtg
    56821 ttgaagattg gtgttcgggt agcggaggaa gaagccgaat cttaagttta atgtgttttg
    56881 taacgctgga tacgacgctg gtcgcatcca gcgataacaa gttacaccgt cacaaccggg
    56941 acgcgtaacg ccagcgcgca catcaactca tagcctaccg ttccggcagc ggcggcgaca
    57001 tcatcaattt tgatctcctt gccccacagc tcaactggcg taccaatacc cgcttgcggg
    57061 caaggcgtta aatcgaccgc cagcatatcc atcgagactg tccccaccgt catggtgcga
    57121 acgccgtcca ctaaaacagg ggtaccggta ggcgcgtggc gcggataacc gtcggcgtat
    57181 cctgcggcga caatgccgat tcgctgttca tcgcgcgcag tatagcgacc gccgtagccc
    57241 acacgctcgc ccgcttttag cgtctggaca ccaataatct cactgcttag cgtcatcacc
    57301 ggacgtaatc cggtattggc gatatcacgc cactgaccgg acggcgaagc gccatacaaa
    57361 ataataccag gccgaaccca gtcaaaatgc gcttcctggt gccacagagt cgccgccgaa
    57421 ttggacaacg aacgccgaca ctccagccct tccgccgcct gctcaatacg cgccatcgcg
    57481 ctggaaattc catcaggatg ttccgcttcg gcaaaatgcg acatcagagt catttcgcca
    57541 acattcgcca ttgcccgcaa ctgctgccag acggtaagca cgcgatcggg ctggaagccc
    57601 aaccgattca tcccactgtt cactttaaga taaatatcca acggtgcttt tagccgcgca
    57661 ttttgcagtg ctttgagctg ccagttgctg tgtacgcagg tggtcaggcg gtgctggtca
    57721 taaatctcca gatcctgagc atggaaaaat ccttccagca tcaggatcgg tcctttccag
    57781 ccgcgctcac gtaacgttat tgcctcttcc aggttaagca atgcaaagcc atcggtagcc
    57841 ccgagcgcgc tccagatacg ctcaataccg tgcccgtaag cgttcgcttt taccaccgac
    57901 cagacgcgcg cgcgcggcgc ggcctggcgg acaatggaca gattctgttt taatgcctgc
    57961 agatcgaggc tggcctgtat cggacgggtc atctcgtttc cttagctgtg tgcgccatgt
    58021 aaatggcccg ggcgtgatgg cgtaaatcca cggctgtagc gcgctacgct tagatcttca
    58081 tacgggatcg ctggcgtgcg ccccgagagc agatcgctta acaactgacc ggaaccgcaa
    58141 gccatcgtcc agccgagcgt gccgtgaccg gtattcagcc acagattttt aaagcgtgta
    58201 cgtccgacaa ccggcgtgcc gtctggcgtc atcgggcgca ggccagtcca gaaagtcgcc
    58261 tgctcaacat gaccgccgcg tggatagaga tcgcgaacca ccatctccag cgtttcacga
    58321 cgcggttgca acagctcggt gttaaagcca acaatttccg ccattccgcc gacacgaatg
    58381 cggttatcga aacgggtaat ggcgattttg taggtttcat caagaatagt ggataccggc
    58441 gcaccatctt cctgcgcaat tggaatggtc agcgagtagc ctttcagcgg atacaccgga
    58501 atatcaacaa tgcctttgag catcgccgtc gagtaagaac caaacgccat cacatacgca
    58561 tcggccttaa tcacttcatc accacacttc acgccgtaaa tttgctcgcc gtcgcaaagc
    58621 agttggtcaa cgggcgtatt aaagcggaat ttaacccccg cctgctccgc catccgcgcc
    58681 agattctggg taaacagctg gcagtctccg gtttcatcat tgggtaactg caggccgccc
    58741 gtcagtttgt gcgccacttc tgccagcgcg ggctccactt ccgccaggcg gctggattcc
    58801 agcagctgat acggtacgcc agcatcttcc agcacggcga tatcgcgggt cgcgttttca
    58861 tactgttgtt cagtacggaa cagttgcagc gtcccaccct gacgcccttc atactgaata
    58921 ttggtttcgg cgcgcaatgc tttcaggcaa tcacggctgt attccgccag acgtaccatc
    58981 cgccctttgt tttccatgta gtggctggtg tcgcagttac gtaacatttg ccacatccat
    59041 ttcaactgga actgcgtacc gtcgagacga accgccagcg gcgcatggcg ctggaacatc
    59101 catttaatcg cttttaaagg cacacctggt gccgcccacg gtgcagcata tccgggggag
    59161 atttgcccgg cattcgcggc actggtttcc agggcggccc ccggctcccg atcaatgacg
    59221 gtgacctcat gtcctgcctg atttaagtac cagacgctgg caacgcctac cacaccactt
    59281 cccagtatga caactcgcat agccactccg ataacggtag atacagtaaa agaataataa
    59341 tctaattaca tcttgataac tcagttgaaa atattattca acatacagct tttttatggt
    59401 gagctggctc acatctccct gactctgcgg caatatggcg tctttagtat ctcctgctga
    59461 acgccctttt tatccagcat aaaattccgt tcagaagcgg attagtggca ctctgataat
    59521 aagaaatcgc aaagaaaaaa attctccggc gacgcggttt ttaacaggtg ttctatgctt
    59581 gaaatgaggt gctccacaca gagagcgccg acaacaatga ggtgcgcgta tggcgacgat
    59641 cgattctatg aataaggaca ccacacgttt gagcgatgga cccgactgga cgttcgacct
    59701 gctggatgtt tatctggcag agatagaccg ggtggcgaaa ctctaccggc tggataccta
    59761 cccgcaccag attgaagtga taacctcaga acagatgatg gatgcctact ccagcgtcgg
    59821 catgccaatt aactatccgc actggtcatt cggtaaaaag tttatcgaga ctgaacggct
    59881 gtataagcac ggtcagcaag gactggccta tgaaatcgtc attaactcta acccgtgtat
    59941 cgcttacctg atggaagaga acaccattac catgcaagcg ctggtgatgg ctcatgcctg
    60001 ctatgggcat aactctttct tcaaaaacaa ttacttattc cgtagctgga ccgacgccag
    60061 ttcgattgtc gattatctga tttttgcccg taaatatatt accgagtgcg aagaacgtta
    60121 tggcgttgat gaagtagaac ggcttctgga ctcgtgccac gcgctgatga actacggcgt
    60181 ggaccggtac aaacgcccgc aaaaaatctc gctgcaagaa gagaaagccc ggcagaaaag
    60241 tcgcgaagag tatctgcaaa gtcaggtcaa tatgctctgg cgtaccctgc cgaagcgcga
    60301 ggaagagaaa acggttgctg aagcgcgccg ctatccgtcc gaaccacaag aaaacctgct
    60361 ctattttatg gagaaaaatg cgccactgct ggaatcatgg cagcgtgaaa tcctgcgtat
    60421 tgtgcgtaag gtgagccagt atttttatcc gcaaaaacag actcaggtga tgaacgaagg
    60481 ctgggcgacc ttctggcact acaccatcct taaccatctg tatgatgaag ggaaagtaac
    60541 ggaacgtttt atgctggagt ttttgcacag ccacaccaat gtggtcttcc agccgcccta
    60601 taacagcccg tggtacagcg gcatcaaccc gtatgccctc gggtttgcca tgttccagga
    60661 tattaaacgg atttgtcagt cgccaacgga agaagacaaa tactggttcc cggatatcgc
    60721 cggttccgac tggctggaaa cgctgcattt cgcgatgcgt gatttcaaag atgagagttt
    60781 tatcagccag ttcctgtcac cgaaagtgat gcgtgatttc cgcttcttca ccgtgctgga
    60841 tgacgatcgg cataattatc tggagatttc cgctattcat aatgaagaag gttatcggga
    60901 gatccgtaac cggttatcgt ctcaatataa tttaagtaat ctggagccga atattcagat
    60961 ctggaacgtg gatttgcgcg gcgaccgttc gctgacgctg cgttacattc cacataatcg
    61021 cgcaccgctg gatcgggggc gcaaagaagt gctgaagcat gtgcatcgcc tgtggggatt
    61081 tgatgtgatg ctggaacagc aaaacgaaga cggcagcgtc gagttgctgg aacgttgccc
    61141 gccgagaatg ggaaatctgt aaatagtcct cctcgtttga ggaggactca tttttgccgg
    61201 atgcggcgta aatgccttat ccggcctaca aagtatcgca aattcaatac attgcaaaaa
    61261 aacacgtagg cctgataagc gtagcgcatc aggcaattat acgtttgtca tccgtctgga
    61321 aggattatcg tccctgaatg gctaaatcac ccggcagatt tttctgcatc cggtgccaaa
    61381 tctcgccact ctcatgccca tagcgacgca ctgtttcgta tacctgatcg tgtgcgcctt
    61441 cactgcacaa cgccgacagt ttgtgataga agcccagcgc cagactgcgc gcttccggat
    61501 tggcgaaata gtgacgacca atgcgcgtat acagcccttt catcccgtta aggatcagac
    61561 cgtaaatcgg gttgccggaa gcaaacgcca ggccgcggaa aatgttgtaa tccagctcgg
    61621 caaaggcgtc ggcgtgatcg gccacttcat tagcggttgc cagcacttcc tgcgctttat
    61681 cgggatgttg acgaaacgcg gtgcgaataa aaatagttga aatattggta cgcaccgaca
    61741 gcaaattatc aataagctgc ggcacacttt cgtggtccag tcgcgccagt gtttcaagga
    61801 tatttaaacc agaggtttcc cagaaattat tcaccttcgt cggcttgccg tgttgaatgg
    61861 tcaaccagcc atctcgtgcc agacgttgta acacttcacg taacgtagta cgcgttacgc
    61921 caattaattc tgaaagttca cgttctgcgg gcaaaatagt cccgggaggg aagcggttat
    61981 tccagatact ttcaataatg tactcttccg cgaaacccgc cgggctttgc gccttaatga
    62041 ccatagtgag atttccataa cacagcaaaa caaagttgga ctcatcatac cagagggcta
    62101 actacgctga tagcagacaa aaagaagaaa aaagggatca aggcttcatt ttccgcatct
    62161 ctttcccctt gactatcaag acttgcctca ctcctgcctg accgttaagc tttgcgttta
    62221 aacataaaaa catcattgat tatttgtaag gtaagggaaa ccatcatgga gatctcctgg
    62281 ggccgcgcgc tatggcgcaa ttttttgggc cagtcccccg actggtacaa actcgccctc
    62341 atcattttct taatcgtaaa tccgttaatt ttcctcatca gccctttcgt cgcgggctgg
    62401 ttgctggtag cggaatttat tttcactctg gcgatggccc tgaaatgcta cccgctgctc
    62461 cccggtggtc tgttggctat cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc
    62521 cgtgaagagg tggcggcaaa tcttgaagtc ttgctgttac tgatgtttat ggtggcgggt
    62581 atctatttta tgaaacagct attgctgttc atatttaccc gtttgctgtt aagcattcgc
    62641 tccaaaatgc tgctgtcgct ctctttttgc gtggcggcgg cgttcctctc cgcgttcctc
    62701 gatgccttaa ccgtcgtggc ggtggtgatc agcgttgccg tcggttttta tggtatttat
    62761 caccgcgtag cctcttcccg tactgaagat accgacctgc aagacgatag tcatatcgac
    62821 aagcattaca aagtggttct ggaacagttc cgtggctttc tgcgtagcct gatgatgcat
    62881 gccggtgttg gcaccgcatt aggcggcgtg atgaccatgg tgggcgaacc acagaacctg
    62941 atcatcgcta aagcggctgg ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg
    63001 accgtcccgg ttctgatttg tggcctgtta acctgcctgc tggtagagaa gctgcgttgg
    63061 tttggctacg gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat
    63121 caaagccgcc accagcgtac ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt
    63181 ggcgtctggc tggtgactgc gctggcgttg catctggcgg aagttggctt gattggtttg
    63241 tcagtcatta ttctggcgac atcattgact ggtgtcaccg atgaacatgc tatcggtaaa
    63301 gccttcaccg aatctctgcc attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg
    63361 gtgattatcg accaacaact gttttcgccg attattcagt ttgtgttgca ggcatcggaa
    63421 catgctcagc tgtcgctgtt ctatattttc aacggtctgc tgtcatccat ttcggataac
    63481 gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc
    63541 acgttgaagc aatacgagct gctggcggtc gccattaata ccggtaccaa tctgccctcc
    63601 gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca
    63661 ttgattcgcc tctcttatgg gcgcatggtg tggatggccc tgccttacac cctcgtcctg
    63721 acactcgtcg gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga atggtttatg
    63781 caaatgggct ggatagcaac gctttgataa caacttaccg ggcatataaa tgcccggttt
    63841 gccttttcgc ccgataattg tccaattgcg tcctattttt gctgcctcct ggtggcgcag
    63901 cgaatgaatt ggtttaaact gcgcactcta tgcatattgc agggaaatga ttatgttgcg
    63961 atttttgaac caatgttcac aaggccgggg cgcatggctg ttgatggcgt ttactgctct
    64021 ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct
    64081 ctgtatttat gaacgctgcg cgttattcgg cgttctgggt gctgcgctga ttggcgcgat
    64141 cgccccgaaa actccgctgc gttatgtagc gatggttatc tggttgtata gtgcgttccg
    64201 cggtgtgcag ttaacttacg agcacaccat gcttcagctc tatccttcgc cgtttgcgac
    64261 ctgtgatttt atggctcgtt tcccggaatg gctgccgctg gataaatggg tgccgcaagt
    64321 gtttgtcgcc tctggcgatt gcgccgagcg tcagtgggaa tttttaggtc tggaaatgcc
    64381 gcagtggctg ctcggtattt ttatcgctta cctgattgtc gcggtgctgg tggtgatttc
    64441 ccagccgttt aaagcgaaaa aacgtgacct gttcggtcgc taatccattc ggcgctcctg
    64501 cgggagcgct ttttcctgcc gctatgttta tttgacccgc agtaaatcag aacttcgcgt
    64561 tgtataacat ggtgaaagca tggcacgctt catctgccat tgttgctgga tcccctgccc
    64621 ggcaaaatag agtgttcctc tgccctcttt agcattcagc gtatccatta ccgccatcaa
    64681 ttgctcactc ccggggcgcg gtgcgttgtc atcgaataaa ttgagctgcg cgactccctg
    64741 actgaagaaa tcccccagca tcacgcccgc tttttggtag cgatggcccg cttgccagat
    64801 ggcatccaga gatcgcgtag cggcgttaat gatatccctg ctgtcctgag tgggcgtcag
    64861 cagttttacc gatgcgctat tgccgtaata aggttcattg aacgcaaatg gtgacgtctt
    64921 aataaacgtg gagataaacc gacaatattg atgctcgctg cgaagttttt ccgccgcccg
    64981 ggcagcgtaa ctacaaatgg cctgccgcat cgacggataa tccgtgatgc gttcaccaaa
    65041 cgagcgggaa cagataattt cctgcttcgt cggtgcaaac tcttccagtt gcaaacaggg
    65101 ttcaccgcgc agttcacgca ccgttctttc gagcacgaca ttaaaatgtt tacggataaa
    65161 ccggatatct gaatccgcca aatcgagaac ggttttgatc cccatcgcgt ccagtttttt
    65221 gctgatccgc cgtccaatcc cccagacttc atccacgggg agagcagaca ttaatttacg
    65281 ctggcgttcc agatttgata aatccaccac cccgcccgtc tgccgctgcc atttttttgc
    65341 cgcatgattg gcaagcttag ccagcgtttt ggtctgggcg atccccacac caacagtaag
    65401 atgggtacgt tgtagcaccg ttgcgcgaat ttctctgcca aaatcagtca gatcgcgaca
    65461 attatgcaca cctgtcagat cgcagaatgc ctcatcaata ctgtaaatct cgacgcgggg
    65521 cgatagttct tccagcgtcg acatcacccg attactcatg tctgcgtaaa gctcataatt
    65581 gctgctaaag caaaccacgc cacagcgacg aaacagatct ttttgtttga accagggatc
    65641 gcccatttta acgcccagcg cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt
    65701 attcgatagc acaaccaccg gtttacccca taaatcaggg cgaaacaccg tctcacagct
    65761 ggcataaaac gcgtttacat cacagagggc aaacatcagc gcatcgcctt aacgacgtgg
    65821 atcaccacac caaagacatc cagcgtatct tcgctactga tggtaatggg cgagtacgca
    65881 ctgttcatag gaataagctg taccgtcggg cgtagttgca attttttcac cgtaaactcg
    65941 ccgtcaacag cagcgatgat aatatcacca tggctggcgg taatagcgct atcgacaatc
    66001 agtaaatcac cgtcactaat cccaccatca atcatagaat caccacttgc tttgacgaaa
    66061 taagtcgcgc tgggatgctg gatcaacagt tgattcagat cgatacgctg ttcaacgtaa
    66121 tctgcggccg gtgaaggaaa gccacactga acaagatcgc taaatagcgg aaaagtcaca
    66181 atttcgcgga gatccgcagg cttgataaac aacataataa tctgcctgaa gttatactgt
    66241 ttttatatac agtagtctgt tcttgtcagc agatcaatac tgattcaggc tatcaatatt
    66301 tgtcgctgca tagactgctg atttttcgtt ctcttatcct gtgctcacgc ggccttctgg
    66361 cgacagcgtc catccagcag aaaggaaaaa tatcacctgg tttaaaatag aatcgaagca
    66421 ttcgcccaga aaggcattct cattaagata ctggatgacg gcatcaagaa actgtgttct
    66481 attgctgcgg gcgtagttga agggaaactg attccagaat tgaagaacaa gttaaaatct
    66541 gtgcagagtt tctttaccac cctgtctaac acggttaaac aagcgaataa agatatcgat
    66601 gccgccaaat tgaaattaac caccgaaata gccgccatcg gggagataaa aacggaaact
    66661 gaaacaacca gattctacgt tgattatgat gatttaatgc tttctttact aaaagaagcg
    66721 gccaaaaaaa taattaacac ttgtaatgag tatcagaaaa gacacggtaa gaagacactc
    66781 tttgagatac ctgaagtctg ataagcgatt attctctcta tgtactcaag gtataaagtt
    66841 tatcacattt aaaaatacag acgtttttta aagattgaga gttacctgtt gataataagg
    66901 tatcgagtaa ctctcattat gacagagcgc tttcaaaaac agggctacga aatggcgagc
    66961 atccagacgt atattaagaa agcccccatc aacatctatt aaatttatca ctgcgcccag
    67021 tcaggtttat ttaagatatg atcctgccag tcaatcactt ctgattcttt tactgcgata
    67081 tgacgcacag agatacgttc accatgcatt gccgctttcg aaccagtaag tagcggatgc
    67141 cacgcaggta aacctttacc ttccgccagc aaacgataag cgcaggtcat tggcagccat
    67201 tcgaatgttg gcagattttc acgggttaat ttaatgcagt cgggttcaaa ctcgaaacga
    67261 cgttcgtagt tccgacattg acaggtttta atattgagct ggcgacaggc gacgttagtg
    67321 aagtagattt cgtcggtgtc ttcatccatc agtttatgca ggcaacactg accgcaacca
    67381 tcacacaacg actcccattc cgcatcgctc atttcgtcca gggttttact ttgccagaaa
    67441 ggtacatcgc tcatcaggtg ttccgccatt acgttaaaac gcaccttata accagtctgg
    67501 cacagcgatg caagttttgc cgccgctttc aggcggcaaa aagtattaca aaacgcgagt
    67561 tgtcaaagaa tgcccatcga aagtgactgt cagctcatca ccgctttgca acgggccgac
    67621 accatcaggc gtgcctgtca gcacaacgtc accggccttg agggtaaaaa acttgctcat
    67681 ataagcgatc agcggaacga ttttatggat catgtccgca gtcgtacctt gctggcgttg
    67741 ctcgccgttt acgctcaggc ccagcgttgt attttgtggg tcgccggtaa actccgccgc
    67801 gggaataaac ccggaaagcg gacaagagtt atcaaacgct ttggcctttt cccacggctg
    67861 cccggctttc ttcatttttc cctgaacatc acgcaatgtc agatcgagcg ccacgccgta
    67921 accggcaatg gctttgcgga catgctcttc cgtagcctga cgcaacgtcg cgccaatcaa
    67981 caccgccagt tcgacttcat gatgaactga accgaaatcg gatgggatcg ccaatggctg
    68041 ccgcagatcg cacagtgccg tttctggttt aataaacagc actggctctt cgggcactgc
    68101 gctgcccatc tctttaatat gtttggcata gttactacca acacagacta ctttactcac
    68161 cggataatcc agcagcgcac cttgccagtt gtgatgttga tacataacgt tcccctcacc
    68221 ttatcgtgga tatgaaaacc cgcgccgaca aagataatgc caccgccgga tatcggttat
    68281 ttgttaatgt cgtctgtttt ttgccccatg acggaaagat gttgcttcag caaatcttcg
    68341 ggtggtggcg gtaactgcaa atagtaacct tgctcggtta atgcctgttt aaccttttca
    68401 atatcggcat taaccagttt cttccgccca tccagcggaa gaatcatcgc taactgaggc
    68461 tgaccaaaac ctttcatcag ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca
    68521 tataaatagg tctggtcacg cttgctgctt cgataaatca cacaaaacat acttttactc
    68581 tttaagatgc caggttttgg cataagctaa catcaatgaa acccgcgcca caagccagcc
    68641 agctgtagat ttctacggtt tgttcagtcc atgccccctt tataagtaat ccccccggca
    68701 aaaatgccgc gccgggattt aaccgaacac aggaaagtca ctggaattct cccgtgaagg
    68761 attgttatat tataacagtt catcgtactc attctgaaca ggagactacc aatgaaaatt
    68821 aagggcatca gtaaggctgt attgttgctt gcactgctaa cctccacgag ctttgctgcg
    68881 ggtaagaatg tcaatgttga gtttagaaaa ggccatagca gcgctcagta ctccggcgaa
    68941 ataaagggat acgattacga tacatatacc ttctatgcca aaaaaggtca gaaagtgcac
    69001 gtgagtatct ctaacgaagg cgctgatact tacctcttcg ggccaggaat cgatgattct
    69061 gttgatttat ccagatattc cccagaactc gatagtcacg gacaatactc actccctgct
    69121 tctggcaaat atgagctgcg agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact
    69181 aaaaaatata acgtcgatat tcaaattaag taaatatcaa ccgacgggga ctctccccgt
    69241 cataaacgtt aacgccccac tcctctctgt cgcttaagag gttaatgacg aatagaacat
    69301 aatgtaaaca agacatcact cacgacacat aaataacatc tttgtggtgg tttgattgat
    69361 ttttccattc cgcttaccat tcacaccaaa ataacgatca tcacgacata atttttcttt
    69421 aacatcgcaa aatacaccgt tagagaatgt aaattcgctg ggatcgtaac cttgtactga
    69481 ttgtaagttt tcagcggctt tttcgccgag atatttcctt gttaactctg gggaaatgcc
    69541 atctgagtta acacaaatat acgggtcgca aacaacgccg cacgccggag attgaagtcc
    69601 gccagcgtca tttccagacg ccattgatga gaacatcatc cccacacctg acaatccgat
    69661 ataaaaaata cgcatcatat tcatagcaca tcctgataat ccaaccgtac aacacttatg
    69721 ctcatttcat catcaatccg taatcaaaac cgctgacctg gacgagttac gatgacctga
    69781 cagaagtatc agacgtcttc tgcggtttct atgatagagc aattctgtgg cggatattaa
    69841 gataacgggt atctgttctt taatgatgct gcaaaaaatg gaaagttaag aaagcctggc
    69901 gtggtgaaaa aatacactcg cctcaacact ggatatttaa acaggcctga agatgctgtc
    69961 acgattaggg tgcggaaaaa ggaagcgtaa ataagaacaa tcaaaagtac gacggcaatg
    70021 ggttgattga caagggtatt ttttaagcta tgaatcagcg ccatttatca cagaatagac
    70081 ttttactctg aataaatggg agggtgactt gcctcaatat aatccagact ataacatgcc
    70141 ttatagtctt cggaacatca tcgcgcgctg gcgatgatta atagctaatt gagtaaggcc
    70201 aggatgtcaa acacgccaat cgagcttaaa ggcagtagct tcactttatc tgtggttcat
    70261 ctgcatgagg cagaacctaa ggttatccat caggcgctgg aagacaaaat cgctcaggcc
    70321 cccgcatttt taaaacatgc ccccgttgta ctcaacgtca gtgcactgga agccccggta
    70381 aactggtcag cgatgcataa ggcggtttcg gcaaccggtt tgcgggttat tggcgtaagt
    70441 ggctgcaaag atgcgcaact taaagccgaa attgaaaaga tggggctgcc tatcctgacg
    70501 gaaggaaagg aaaaagcgcc gcgtccagct cccgcaccgc aggctccagc gcaaaataca
    70561 acgccggtca caaaaacgcg tttaattgat accccagtgc gttccggtca gcgtatttat
    70621 gctccacaat gtgatctgat tgttacaagc cacgttagcg caggggccga attgattgcc
    70681 gatggtaaca ttcatgtcta tggcatgatg cgcggtcgtg cgctggcagg ggcaagtggt
    70741 gaccgggaaa cgcaaatatt ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt
    70801 gaatactggc tgagtgatca aatcccagca gaattttatg gcaaagcggc acgactgcag
    70861 ttagtcgaaa acgctttgac cgttcaaccg ttaaattgat ccctttttaa caaggaattt
    70921 ctatggcacg cattattgtt gttacttcgg gcaaaggggg tgttggtaag acaacctcca
    70981 gcgcggccat cgccactggt ttggcccaga agggaaagaa aactgtcgtg atagattttg
    71041 atatcggcct gcgtaatctc gacctgatta tgggttgtga acgccgggtc gtttacgatt
    71101 tcgtcaacgt cattcagggc gatgcaacgc taaaccaggc gttaattaaa gataaacgta
    71161 ctgaaaatct ctatattctt ccggcatcgc aaactcgcga taaagatgcc ctcacccgtg
    71221 aaggggtcgc caaagttctt gatgatctga aagcgatgga ttttgaattt atcgtttgtg
    71281 actccccggc agggattgaa accggtgcgt taatggcact ctattttgca gacgaagcca
    71341 ttattaccac caacccggaa gtctcctcag tacgcgactc tgaccgtatt ttaggcattc
    71401 tggcgtcgaa atcacgccgc gcagaaaatg gcgaagagcc gattaaagag cacctactgt
    71461 taacgcgcta taacccaggc cgcgtaagca gaggtgacat gctgagcatg gaagatgtgc
    71521 tggagatcct gcgcatcaaa ctcgtcggcg tgatcccaga ggatcaatca gtattgcgcg
    71581 cctctaacca gggtgaaccg gtcattctcg acattaacgc cgatgcgggt aaagcctacg
    71641 cagataccgt agaacgtctg ttgggagaag aacgtccttt ccgcttcatt gaagaagaga
    71701 agaaaggctt cctcaaacgc ttgttcggag gataagttat ggcattactc gatttctttc
    71761 tctcgcggaa gaaaaacaca gccaacattg caaaagaacg gttgcagatt attgttgctg
    71821 aacgccgtcg cagcgatgca gaaccgcatt atctgccgca gttgcgtaaa gatattcttg
    71881 aggtcatttg taaatatgta caaattgatc ctgagatggt aaccgtacag cttgagcaaa
    71941 aagatggcga tatttctatt cttgagctga acgtgacctt accggaagca gaagagctga
    72001 aataagcccc ctgtaaaagc gcatttatct tcaaggcaga gtcatctctg ccttgagttt
    72061 ctgtgcacaa taatattgat aaatcagatt atcttacctt tacaacattg cgttaacagt
    72121 caatgacact tcttgaataa aaatattgtc aatcaatgag ttatttatca tttgagatta
    72181 ttctcattcc cgtaaaaaac ataattccta tcatcacagt cacgctcatt ttgaattatc
    72241 tgaataatag cgagcgttct ttagttaagc agatcttaat tgaagacgaa attattgtgt
    72301 gtgcaactta cttaataccg gacaactaag ttgaagaaaa aaatacttgc cttcgggctt
    72361 atctcagcat tgttctgctc cacgccagcg atggcggata tgaatcgcac cacgaaaggt
    72421 gcattgctag gcgcaggcgt tggtttactt actggcaatg gtgttaatgg cgtactgaaa
    72481 ggtgcagctg ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat
    72541 gcgcgtaaag gtgcaaaagt gggtgccgcc gttggtgctg tcactggcgt tctgaccggc
    72601 aatggcctcg aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg
    72661 ggtaaaatga aataaggaat gatgatgaaa aaatttgctt tactggcggg tctgtttgtt
    72721 ttcgcaccaa tgacctgggc gcaggactac aatattaaga acggtttgcc atcagaaacc
    72781 tatattactt gtgcagaagc caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa
    72841 attgttgcgg taatgggtaa tgccagcgtt gccagccgtg atttaaaaat tgagcaatct
    72901 ccggaactga gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa agatccgcaa
    72961 atgctgttga tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa ataatatttc
    73021 atatgttacc gcgtcgatga attcgacgcg gttttttagc taacgggcgg gatcagaact
    73081 tatatttcag gcccaccatc attgcggtgt cattgtagcc attatcaccc agctgcacgc
    73141 ccacattacc ccagacgctg gccgccggat tcagattgcc atttacgcca tgtttcattt
    73201 cccccagatt acgcgccccg tcttgttcaa ctttcacacc gtttattgag acagcaaaat
    73261 ctttactgtt atggatcccg ttcatttcaa caaaaggttc aaagccgtca ccgtgcggac
    73321 cgctgttttt ctcctgagta cgaataaagg tgcggaaaac caagtcggat ctgaatgttg
    73381 ttatttgcgt cattctcaat acgtgagcca ttggcttccg tgtgcgcatt atgatcgacg
    73441 cccatccaga tggcctgtgc cgaggtcgtt ctcaactgtc cgttgctgtc acgccaggca
    73501 ttatgcccgc caatttgacg taaccaaagt tggcttgaac gctcctgttc agttacagga
    73561 tcgatatagc gcgtttcacc tgcacaatcg ttcagacgca taacaaaaag agtgttagcg
    73621 gctgccaggt tggcgacgta actgccagct tcgggtcgca ggacgggggt aggcgtttct
    73681 gttgattctg aagtgacatt ggaaaaaaat aactctctac cattgtatat ttttgcaatg
    73741 ggggctttag tgccatttgt tgtaatagaa cttttttata tcaatgttac cttgtcgaat
    73801 agcaactgca taacttccat cagccacagt ttcaagtacc acataatcta aattaatata
    73861 tgcttttttc ccattagcat aagcaccata gctattaata ccattagttg ttatggaacc
    73921 accattcatt tttaaatcag cacggctaat aacttcgtaa ctgttagaat cacaaacttc
    73981 atcatctaca gcatcgacac atgttcgaga ctctccagga cttgaacatg caagaactcc
    74041 tactgcattt ttcccatagg taacaatgtt cgttttatct gtggtttcaa gactggtgtt
    74101 attttccaca gtctcagaat cattaacctg acttaaaagt cccgctgaat atgccccctc
    74161 gtctcgactg aattatcacc attcataacg atggtgccac caaattcact gactactgcg
    74221 ctggctgtta tccctccatt ttctttgtca gatacgcttt cagagcgtct taatattgct
    74281 atcgcttata tataatactt tgttttggct atcaacagaa ataccataag atccatcacc
    74341 actagtcatc acactggcat tatgtatata tccagttaca ccgtcatcca ctttcacggc
    74401 atggctttcg tcttaagtga ttagatactc cccattactt gtagaagtat tatttttgat
    74461 ttcaatcggt gattcttcat cggttatata ggaaattata ctataattat ccaacaattg
    74521 tgttgcatat gcattaccca ccgccagagc aataagtcct aaactgaacc aggggagttt
    74581 tttcagtttc atagattaac catccattca ttggattaac aaacattcct ggtcaaatac
    74641 cctatgagtt cagattacca aaacggaagg caaagataaa aacaaaagca caagaaattt
    74701 aaatagataa acagcgacaa tatttaaatg ataaataagc cagcattatc atttttagct
    74761 tattttcatg acattgatca ctt
//
LOCUS       DAZFRE010000013        69904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFRE010000013 DAZFRE010000000
VERSION     DAZFRE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.013"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            29..304
                     /gene="ypdI"
                     /locus_tag="ACHKUI_001031"
     CDS             29..304
                     /gene="ypdI"
                     /locus_tag="ACHKUI_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO6597281.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITSTYPTKANYLIHPLR"
     gene            complement(381..623)
                     /gene="yfdY"
                     /locus_tag="ACHKUI_001032"
     CDS             complement(381..623)
                     /gene="yfdY"
                     /locus_tag="ACHKUI_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6597282.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            976..1896
                     /gene="lpxP"
                     /locus_tag="ACHKUI_001033"
     CDS             976..1896
                     /gene="lpxP"
                     /locus_tag="ACHKUI_001033"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6597283.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2252..2323
                     /gene="ypdK"
                     /locus_tag="ACHKUI_001034"
     CDS             2252..2323
                     /gene="ypdK"
                     /locus_tag="ACHKUI_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6597284.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHKUI_001035"
     CDS             complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHKUI_001035"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6597285.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4002..5699
                     /gene="ypdA"
                     /locus_tag="ACHKUI_001036"
     CDS             4002..5699
                     /gene="ypdA"
                     /locus_tag="ACHKUI_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6597286.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIEVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5714..6448
                     /gene="ypdB"
                     /locus_tag="ACHKUI_001037"
     CDS             5714..6448
                     /gene="ypdB"
                     /locus_tag="ACHKUI_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6597287.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6461..7318
                     /gene="ypdC"
                     /locus_tag="ACHKUI_001038"
     CDS             6461..7318
                     /gene="ypdC"
                     /locus_tag="ACHKUI_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6597288.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7321..9816)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_001039"
     CDS             complement(7321..9816)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_001039"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6597289.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9841..10878)
                     /gene="ypdE"
                     /locus_tag="ACHKUI_001040"
     CDS             complement(9841..10878)
                     /gene="ypdE"
                     /locus_tag="ACHKUI_001040"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6597290.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10878..11963)
                     /gene="ypdF"
                     /locus_tag="ACHKUI_001041"
     CDS             complement(10878..11963)
                     /gene="ypdF"
                     /locus_tag="ACHKUI_001041"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6597291.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHKUI_001042"
     CDS             complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHKUI_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6597292.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHKUI_001043"
     CDS             complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHKUI_001043"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6597293.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHKUI_001044"
     CDS             complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHKUI_001044"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6597294.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHKUI_001045"
     CDS             14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHKUI_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6597295.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAITIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPALFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHKUI_001046"
     CDS             16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHKUI_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6597296.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHKUI_001047"
     CDS             complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHKUI_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6597297.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18373..19575
                     /gene="nupC"
                     /locus_tag="ACHKUI_001048"
     CDS             18373..19575
                     /gene="nupC"
                     /locus_tag="ACHKUI_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6597298.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19625..21814)
                     /gene="pdeA"
                     /locus_tag="ACHKUI_001049"
     CDS             complement(19625..21814)
                     /gene="pdeA"
                     /locus_tag="ACHKUI_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6597299.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSSHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKIRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLIMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22021..22096)
                     /locus_tag="ACHKUI_001050"
     tRNA            complement(22021..22096)
                     /locus_tag="ACHKUI_001050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22061..22063),aa:Ala,seq:ggc)
     gene            complement(22136..22211)
                     /locus_tag="ACHKUI_001051"
     tRNA            complement(22136..22211)
                     /locus_tag="ACHKUI_001051"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22176..22178),aa:Ala,seq:ggc)
     gene            22432..22791
                     /gene="yfeC"
                     /locus_tag="ACHKUI_001052"
     CDS             22432..22791
                     /gene="yfeC"
                     /locus_tag="ACHKUI_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6597300.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22793..23185
                     /gene="yfeD"
                     /locus_tag="ACHKUI_001053"
     CDS             22793..23185
                     /gene="yfeD"
                     /locus_tag="ACHKUI_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6597301.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23236..24651)
                     /gene="gltX"
                     /locus_tag="ACHKUI_001054"
     CDS             complement(23236..24651)
                     /gene="gltX"
                     /locus_tag="ACHKUI_001054"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6597302.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24910..24985
                     /locus_tag="ACHKUI_001055"
     tRNA            24910..24985
                     /locus_tag="ACHKUI_001055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24943..24945,aa:Val,seq:tac)
     gene            25030..25105
                     /locus_tag="ACHKUI_001056"
     tRNA            25030..25105
                     /locus_tag="ACHKUI_001056"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25063..25065,aa:Val,seq:tac)
     gene            25152..25227
                     /locus_tag="ACHKUI_001057"
     tRNA            25152..25227
                     /locus_tag="ACHKUI_001057"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25185..25187,aa:Val,seq:tac)
     gene            25232..25307
                     /locus_tag="ACHKUI_001058"
     tRNA            25232..25307
                     /locus_tag="ACHKUI_001058"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25265..25267,aa:Lys,seq:ttt)
     gene            complement(25418..26302)
                     /gene="xapR"
                     /locus_tag="ACHKUI_001059"
     CDS             complement(25418..26302)
                     /gene="xapR"
                     /locus_tag="ACHKUI_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO6597303.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(26343..26501)
                     /locus_tag="ACHKUI_001060"
     CDS             complement(26343..26501)
                     /locus_tag="ACHKUI_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO6597304.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(26554..27810)
                     /gene="xapB"
                     /locus_tag="ACHKUI_001061"
     CDS             complement(26554..27810)
                     /gene="xapB"
                     /locus_tag="ACHKUI_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO6597305.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(27871..28704)
                     /gene="xapA"
                     /locus_tag="ACHKUI_001062"
     CDS             complement(27871..28704)
                     /gene="xapA"
                     /locus_tag="ACHKUI_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO6597306.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGGPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            28952..29716
                     /gene="yfeN"
                     /locus_tag="ACHKUI_001063"
     CDS             28952..29716
                     /gene="yfeN"
                     /locus_tag="ACHKUI_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6597307.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(29755..30681)
                     /gene="yfeR"
                     /locus_tag="ACHKUI_001064"
     CDS             complement(29755..30681)
                     /gene="yfeR"
                     /locus_tag="ACHKUI_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6597308.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            30771..31769
                     /gene="yfeH"
                     /locus_tag="ACHKUI_001065"
     CDS             30771..31769
                     /gene="yfeH"
                     /locus_tag="ACHKUI_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6597309.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(31766..31984)
                     /gene="ypeB"
                     /locus_tag="ACHKUI_001066"
     CDS             complement(31766..31984)
                     /gene="ypeB"
                     /locus_tag="ACHKUI_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6597310.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(31986..34001)
                     /gene="ligA"
                     /locus_tag="ACHKUI_001067"
     CDS             complement(31986..34001)
                     /gene="ligA"
                     /locus_tag="ACHKUI_001067"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6597311.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTFCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(34072..35070)
                     /gene="zipA"
                     /locus_tag="ACHKUI_001068"
     CDS             complement(34072..35070)
                     /gene="zipA"
                     /locus_tag="ACHKUI_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6597312.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            35300..36061
                     /gene="cysZ"
                     /locus_tag="ACHKUI_001069"
     CDS             35300..36061
                     /gene="cysZ"
                     /locus_tag="ACHKUI_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6597313.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNIFLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36246..37217
                     /gene="cysK"
                     /locus_tag="ACHKUI_001070"
     CDS             36246..37217
                     /gene="cysK"
                     /locus_tag="ACHKUI_001070"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6597314.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            37601..37858
                     /gene="ptsH"
                     /locus_tag="ACHKUI_001071"
     CDS             37601..37858
                     /gene="ptsH"
                     /locus_tag="ACHKUI_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6597315.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            37903..39630
                     /gene="ptsI"
                     /locus_tag="ACHKUI_001072"
     CDS             37903..39630
                     /gene="ptsI"
                     /locus_tag="ACHKUI_001072"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6597316.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            39671..40180
                     /gene="crr"
                     /locus_tag="ACHKUI_001073"
     CDS             39671..40180
                     /gene="crr"
                     /locus_tag="ACHKUI_001073"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6597317.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(40223..41074)
                     /gene="pdxK"
                     /locus_tag="ACHKUI_001074"
                     /gene_synonym="thiJ"
     CDS             complement(40223..41074)
                     /gene="pdxK"
                     /locus_tag="ACHKUI_001074"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6597318.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            41179..41547
                     /gene="yfeK"
                     /locus_tag="ACHKUI_001075"
     CDS             41179..41547
                     /gene="yfeK"
                     /locus_tag="ACHKUI_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO6597319.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(41550..42461)
                     /gene="cysM"
                     /locus_tag="ACHKUI_001076"
     CDS             complement(41550..42461)
                     /gene="cysM"
                     /locus_tag="ACHKUI_001076"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6597320.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANCGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(42596..43693)
                     /gene="cysA"
                     /locus_tag="ACHKUI_001077"
     CDS             complement(42596..43693)
                     /gene="cysA"
                     /locus_tag="ACHKUI_001077"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6597321.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(43683..44558)
                     /gene="cysW"
                     /locus_tag="ACHKUI_001078"
     CDS             complement(43683..44558)
                     /gene="cysW"
                     /locus_tag="ACHKUI_001078"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6597322.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(44558..45391)
                     /gene="cysT"
                     /locus_tag="ACHKUI_001079"
     CDS             complement(44558..45391)
                     /gene="cysT"
                     /locus_tag="ACHKUI_001079"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6597323.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(45391..46407)
                     /gene="cysP"
                     /locus_tag="ACHKUI_001080"
     CDS             complement(45391..46407)
                     /gene="cysP"
                     /locus_tag="ACHKUI_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6597324.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(46565..47356)
                     /gene="ucpA"
                     /locus_tag="ACHKUI_001081"
     CDS             complement(46565..47356)
                     /gene="ucpA"
                     /locus_tag="ACHKUI_001081"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6597325.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            47636..48532
                     /gene="murQ"
                     /locus_tag="ACHKUI_001082"
     CDS             47636..48532
                     /gene="murQ"
                     /locus_tag="ACHKUI_001082"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6597326.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTTIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            48536..49960
                     /gene="murP"
                     /locus_tag="ACHKUI_001083"
     CDS             48536..49960
                     /gene="murP"
                     /locus_tag="ACHKUI_001083"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6597327.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            49963..50049
                     /locus_tag="ACHKUI_001084"
                     /pseudo
     CDS             49963..50049
                     /locus_tag="ACHKUI_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(50138..51037)
                     /gene="yfeX"
                     /locus_tag="ACHKUI_001085"
     CDS             complement(50138..51037)
                     /gene="yfeX"
                     /locus_tag="ACHKUI_001085"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6597328.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(51133..51708)
                     /gene="yfeY"
                     /locus_tag="ACHKUI_001086"
     CDS             complement(51133..51708)
                     /gene="yfeY"
                     /locus_tag="ACHKUI_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6597329.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(51769..52218)
                     /gene="yfeZ"
                     /locus_tag="ACHKUI_001087"
     CDS             complement(51769..52218)
                     /gene="yfeZ"
                     /locus_tag="ACHKUI_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6597330.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRALFPRIWHVLYFLLLLAQVVL
                     LCWQPWLRLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(52205..52630)
                     /gene="ypeA"
                     /locus_tag="ACHKUI_001088"
     CDS             complement(52205..52630)
                     /gene="ypeA"
                     /locus_tag="ACHKUI_001088"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6597331.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            52844..53713
                     /gene="amiA"
                     /locus_tag="ACHKUI_001089"
     CDS             52844..53713
                     /gene="amiA"
                     /locus_tag="ACHKUI_001089"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6597332.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            53717..54616
                     /gene="hemF"
                     /locus_tag="ACHKUI_001090"
     CDS             53717..54616
                     /gene="hemF"
                     /locus_tag="ACHKUI_001090"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6597333.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54622..55674)
                     /gene="eutR"
                     /locus_tag="ACHKUI_001091"
     CDS             complement(54622..55674)
                     /gene="eutR"
                     /locus_tag="ACHKUI_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6597334.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(55720..56220)
                     /gene="eutK"
                     /locus_tag="ACHKUI_001092"
     CDS             complement(55720..56220)
                     /gene="eutK"
                     /locus_tag="ACHKUI_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6597335.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(56233..56892)
                     /gene="eutL"
                     /locus_tag="ACHKUI_001093"
     CDS             complement(56233..56892)
                     /gene="eutL"
                     /locus_tag="ACHKUI_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6597336.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(56902..57789)
                     /gene="eutC"
                     /locus_tag="ACHKUI_001094"
     CDS             complement(56902..57789)
                     /gene="eutC"
                     /locus_tag="ACHKUI_001094"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6597337.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(57810..59171)
                     /gene="eutB"
                     /locus_tag="ACHKUI_001095"
     CDS             complement(57810..59171)
                     /gene="eutB"
                     /locus_tag="ACHKUI_001095"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6597338.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(59183..60586)
                     /gene="eutA"
                     /locus_tag="ACHKUI_001096"
     CDS             complement(59183..60586)
                     /gene="eutA"
                     /locus_tag="ACHKUI_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6597339.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGGQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPTDP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(60583..61770)
                     /gene="eutH"
                     /locus_tag="ACHKUI_001097"
     CDS             complement(60583..61770)
                     /gene="eutH"
                     /locus_tag="ACHKUI_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6597340.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGVLAGIVTIPIGCIAGG
                     LVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPEKMINGFQIFAKFL
                     VALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEVIGSISCVLLGAYP
                     MVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMKQMDTRGKVINCAF
                     AVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLVPKEDATAAKTEAE
                     AQS"
     gene            complement(62287..63474)
                     /gene="eutG"
                     /locus_tag="ACHKUI_001098"
     CDS             complement(62287..63474)
                     /gene="eutG"
                     /locus_tag="ACHKUI_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6597341.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(63464..64300)
                     /gene="eutJ"
                     /locus_tag="ACHKUI_001099"
     CDS             complement(63464..64300)
                     /gene="eutJ"
                     /locus_tag="ACHKUI_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6597342.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRNGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(64311..65714)
                     /gene="eutE"
                     /locus_tag="ACHKUI_001100"
     CDS             complement(64311..65714)
                     /gene="eutE"
                     /locus_tag="ACHKUI_001100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO6597343.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDECGKGTVSRDWVGRDAAKIA
                     AAIDLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(65726..66013)
                     /gene="eutN"
                     /locus_tag="ACHKUI_001101"
     CDS             complement(65726..66013)
                     /gene="eutN"
                     /locus_tag="ACHKUI_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6597344.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(66120..66413)
                     /gene="eutM"
                     /locus_tag="ACHKUI_001102"
     CDS             complement(66120..66413)
                     /gene="eutM"
                     /locus_tag="ACHKUI_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6597345.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(66452..67468)
                     /gene="pta"
                     /locus_tag="ACHKUI_001103"
     CDS             complement(66452..67468)
                     /gene="pta"
                     /locus_tag="ACHKUI_001103"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6597346.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     KQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNHESSLQ
                     TLVE"
     gene            complement(67465..68268)
                     /gene="eutT"
                     /locus_tag="ACHKUI_001104"
     CDS             complement(67465..68268)
                     /gene="eutT"
                     /locus_tag="ACHKUI_001104"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6597347.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(68265..68966)
                     /gene="eutQ"
                     /locus_tag="ACHKUI_001105"
     CDS             complement(68265..68966)
                     /gene="eutQ"
                     /locus_tag="ACHKUI_001105"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6597348.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(68941..69420)
                     /gene="eutP"
                     /locus_tag="ACHKUI_001106"
     CDS             complement(68941..69420)
                     /gene="eutP"
                     /locus_tag="ACHKUI_001106"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6597349.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQTVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(69433..69768)
                     /gene="eutS"
                     /locus_tag="ACHKUI_001107"
     CDS             complement(69433..69768)
                     /gene="eutS"
                     /locus_tag="ACHKUI_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6597350.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        16814 a        19183 c        17454 g        16453 t
ORIGIN      
        1 tcagtgaacc attttcagga tattttctat gaaagtaaac ttaatgcttt ttagcttatt
       61 tttatttgtc tctattatgg catgcaatgt ttttgcattt tctatttctg gtggtggtag
      121 tgaggcgagc tataaagaga cagaaaaaac gtcagtgatg acgacaacgc attctacaaa
      181 acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcaccac cattaaacct
      241 cacagaagac atcacgtcta cttatccgac aaaggcgaat tatctcattc atcctttgcg
      301 atagcgtctg tcacctggca ggtgaacaat catgtctgat gctgatgtga acacctgccc
      361 aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag
      421 ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac
      481 cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagatc
      541 gccgatacca tgttcagtac ctgaccgatg tatccgctta cgcagacaat acacagggcg
      601 agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt
      661 cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatca
      721 aaacagcatg ttaaatgcaa gactgttacg tacggaaaaa tatttacttt gcacgattaa
      781 taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat tcatgctcct
      841 cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg
      901 cctaatcaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat
      961 ctacagaagg taactatgtt tccaaaatgc aaattttccc gcgagtttct gcatcctcgc
     1021 tactggctca cgtggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct
     1081 gttctctgct ttctcggcac gcgtatcggc acaatggcgc gaccattcct gaaacgtcgt
     1141 gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc gaaagagcgc
     1201 gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg
     1261 gcgtggttct ggcccgacag tcgcgtacgt aaatggtttg atgtggaagg gctggataac
     1321 cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg
     1381 ctggaactgg gcggtcgggt aatgggactt tgccaaccaa tgatggccac ctatcgcccg
     1441 cataataatc agctgatgga atgggtgcag acccgtggtc gcatgcgctc taacaaagcg
     1501 atgatcggca gaaataatct gcgcggcatt gtcagtgcac tgaagaaagg tgaagcggta
     1561 tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg
     1621 gtggaaaatg tcgccacaac caatggcacc tatgtcctct ctcgtctctc tggcgcagcc
     1681 atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt gtacatcacc
     1741 ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt
     1801 atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa
     1861 acgcgcccgg tgggtgaatc gtcgttatac atttaacaaa cggcgctcac tctttccctc
     1921 ctcaaatcag ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt
     1981 cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacctgta
     2041 agttactaaa agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta
     2101 gcgccgtctc gagcagttta gctcagaatt ctgggatgat gcccacggta aaatacagga
     2161 cttttaaggt cgttttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag
     2221 gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt
     2281 tatggtcatc gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg
     2341 aaaaacagga gccatcaggc tcctgtttgc atttctggcg aaggcgttat tccgcgtttt
     2401 cgtgaatatg tttgctgctg gcaggtagta aaccgtcggc acggaacatc gctttaatcc
     2461 cacgaatcgc ctgacgaata cggtcgcggt tttcaatcag tgcaaagcgc acatgcgtgt
     2521 cgccgtagtc gccaaagcca atccccggag agacacagac cttcgcttcg ttaagcaatt
     2581 tcttggcaaa ttccagcgag cccatggcag catacggttc cgggattttc gcccagacat
     2641 acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttcaccagca
     2701 catcacggcg gcgtttgtac tgttcggcaa tatcacgcac gcactgttga tcgccctcca
     2761 gcgccgcaat agccgccacc tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa
     2821 tacgtgccaa cgcgctgacc agcgttttgt taccgaccat aaagccgata cgccagcccg
     2881 ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacgtcgcgt gcacctggca
     2941 cctgcatgat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcgt
     3001 ggaccaccag cacatcgtag cgtttagcca gcgccacaac cttctcaaag aattccagtt
     3061 ccacgcattg cgcggttggg tttgacggga agccgaggat catcatcttc ggtttcggat
     3121 aactttcacg aatggcgcgc tccagttcat tgaagaagtc gacaccttcc accagcggca
     3181 ctgagcgtac ctgcgccccg gcaatcaccg cgccgtaaat atgaatgggg tagcttggat
     3241 tcggcaccag cactgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct
     3301 ctttcgaacc aatagtgacg atggcttctg attctgggtc gatttcaacg tcgtagcgat
     3361 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgac gtggagtaac
     3421 cgtgcgtgtc cgggcgctgg gccaccgtac ataatttttc gacgatgtgc ggcggagtcg
     3481 caccgtccgg gttacccata ctgaaatcga taatatcttc gccgcgccga cgcgcagcca
     3541 ttttcagttc agcggtaatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc
     3601 gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc
     3661 cgagcgacgc tgccacgatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
     3721 cagcgtttta gaacattttt atcaatggct ttaaatgagg aactccactt caaaaacagg
     3781 aaatttatct acgctaacaa gaaaattacc actcatttta ttaacattat aatttcaata
     3841 aatttaccaa ccagatgtgc attatcaaga gatgcattgc taatacacca tttatctttc
     3901 aatccccttt taaaattgtg gtttttcctc aaaaacggcc tgctggtcat cagccttcag
     3961 gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca
     4021 tgctgctggc ggtcttcgat cgggccgcgt taatgcttat ctgcctgttt tttctcatcc
     4081 gtatccgcct gtttcgcgaa ctgttgcaca agtcggcaca ctcaccgaaa gagctgctcg
     4141 ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag
     4201 aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc aggcggaatt ctgtttggcc
     4261 cgtgggtggg catcattacc ggcgtgattg cgggtattca ccgttattta attgacatcg
     4321 gcggcgtaac ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcgggct
     4381 ggatcaacct gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt
     4441 tgtgtgaaac gttgaccatg attctggtga ttgtctgggc accaactacc gcattgggga
     4501 tcgatatcgt ctctaaaatc gggattccaa tgatcctcgg cagcgtctgt atcggcttta
     4561 ttgtgttgct ggtacaaagt gttgagggag aaaaagaggc cagcgccgcg cggcaggcca
     4621 agctggcgct ggatatcgcc aacaaaacgc tgccgctgtt tcgccatgtc aatagcgagt
     4681 cattacgcaa agtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatga
     4741 ctaataccga tcatgtgctg gcctacgttg gcgtgggtga acataactat cagaatggcg
     4801 atgacttcat tagcccgact acccgccagg cgatgaatta tggaaaaatc atcattaaaa
     4861 acaatgatga agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg
     4921 agaaaggcgt tgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagataacct
     4981 cgtcattaca ggaaatggcg gtcggtctgt cacaaatcat ctccacgcaa ctggaggtgt
     5041 cacgcgccga gcagctgcgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca
     5101 aaattaatcc ccatttcctg tttaacgctt tgaacgctat ttcatcgtca atccgcctga
     5161 atccggatac tgctcgccag ctgatcttta atctgtcgcg ttatctgcgc tataacattg
     5221 aattaaaaga tgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata
     5281 ttgccattga gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag
     5341 aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggaaaac gccattgtcc
     5401 acggtatcca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggta
     5461 atcgggtacg tattgaggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc
     5521 gggtcgaagc gaacgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg
     5581 tgaagttatt gtatggcgag gggctgcata tccgccgcct ggaaccagga acggaaattg
     5641 cgttttacat tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag
     5701 ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact
     5761 gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct
     5821 ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat
     5881 tccgtcgctg gatggtgtgt tgctggcaca aaacatcagc cagttcgccc ataaaccgtt
     5941 tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaagcgtt
     6001 tgactacatt ctcaaaccgt atcaggagtc acgcattacc gggatgctgc aaaaactgga
     6061 agcggcctgg caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa
     6121 tgacaccatt aatctggtga aagatgagcg aatcatcgtc acgcccatta acgatatcta
     6181 ttacgccgaa gcgcatgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat
     6241 gccgatgaac attaccgagt tttgcagcaa gctgccgccg tcgcattttt tccgctgcca
     6301 tcgctcattt tgtgtcaatc tgaacaaaat ccgcgagatc gaaccgtggt ttaataacac
     6361 ctatattctg cggctgaaag atctggattt tgaagtgccg gtcagccgca gcaaagttaa
     6421 agaattccgc cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc
     6481 cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc cgcaactact
     6541 ggggcgcgtc tggtttgcca gccagcctac ctcattgcca gtgggcagtt tatgtattga
     6601 ttttccccgt ctggatatcg tgctacgcgg cgaatacggc aatctgctgg aagcaaagca
     6661 gcaacgtatg gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccggt
     6721 caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttggcttatc
     6781 attttacgat agccgcacca cgtcgttgtt acatcctgct cgccagatcc aacttcccag
     6841 cctgcaacgc ggcgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc
     6901 gctggagcag aatatcattc agccactggt gttaagtttg ctgcatcttt gccgtaacgt
     6961 ggtgaatatg ccgccgggca attcgcagcc gcgcggcgat tttctctatc acagcatttg
     7021 taactgggtt caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt
     7081 taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacaa tgcgttttat
     7141 cgagtatgtt cgctgggtac gaatggcaaa agcgaggatg attttgcaaa aatatcatct
     7201 gtcgattcat gaagtggcgc agcgttgcgg gtttccggat agcgactatt tttgtcgcgt
     7261 tttccggcgt cagtttggtc tgacgccggg agagtatagc gcacgttttc agggctaacg
     7321 tcagaaggtt aattccgttt ccagcagcgt cagaatactt tgtgcatctt gcgcggcaaa
     7381 cagcgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac
     7441 atgattcatc ccttcgttag cgcccagcgt cagcatgatc accagttcga cttcgcccat
     7501 ttctgactgc cagtcaaccg gtttcaccaa ccgggcaatg ctgatactgg aatgacggat
     7561 ccactgtgat ttggtgtgcg ggatcgccac gccaaaacca acgccagtag taacaatctc
     7621 ttcccgctgc cagacatctt cttccagctc gaacggatgt tcagtacgcc cgttaacacc
     7681 gaggttaccg cacaggaact ggatcgcctg ctctttatta ctaaaggact gatcaacaaa
     7741 gatattctct agtgccagca gtgggcgaac gtcttcttcc ggcgcaaacg cggtaagtaa
     7801 cgcttcaatt tcctgcgcgc tgcggcactc gcacgcctga cgcgccagtt cccgacacgc
     7861 ctcgctatcc agttgacgaa gctggctttt cactgccgga atacgcgggc tactcatgct
     7921 tagctcatcc aggcccagcc ctagcagtag cggcagataa cgactttcac cgcccagttc
     7981 accgcaaata cctacccatt tgccgcgctg atgcgcagcg gtaacaatct gctgcaacat
     8041 gcgcaggaac gatggcgtaa tcgggttata cagcggcgat acgcgcgggt tattacgatc
     8101 gactgcatac agatactggg tcatatcgtt agaaccgata ctgaagaaat cgacctcatc
     8161 gcagaagtgg tcgatgatgt agcacaccga cggcacttcc accatgatac ccagcgtaat
     8221 cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggagctcacc
     8281 cttcacccat aagatctgat cgagactgtg gaccattggg atcatcaact gggcgttgcc
     8341 gaaactggca gcgcgcaaaa tcgcccgcaa ttgagtacgg aacaggccag caaattccgg
     8401 gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa tgttgaggta
     8461 aggaatgctt ttatcgccac cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc
     8521 cgccagtagc acctgctggt acgcttcaaa ctgttcctgc tcgtccggcg cgctgtcgcg
     8581 atccatatag agcatttcgg tacgaaacag cccaacgcct tccgcgccgt tagcaaacgc
     8641 gcctggggct tccagagcgg tgccaatatt cgccgcaata tcaatacgtt tgttatcacg
     8701 ggaataggcc agctgcgcgg cagcttgcgc ctgttgtttt tggcgtttat ccgccagcgt
     8761 ctgcgccacc tggtaataac cgctcaccgc atcgttggga ttaattgcca gtacgccgca
     8821 ctgggcatca agcactgcag gttgcccggc ataacgggca atcgcatcca gcggcaggcc
     8881 gctcagtacc gggatcgccg aagcgcgggc cagaatcagg gtatgcgagg tgcgcccggt
     8941 tttctccaga atcatgcccg caagattttt taaatcgagg ctcaaaaact ggcttggggt
     9001 taaatcttca gccaccagaa tggtcggttt ttcaagcacc agattattgc gcggttttag
     9061 ttccggccag gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc
     9121 gcgcagataa tcgctggcag aagcagaaag tttggcacaa atctgctcca tattgctgat
     9181 gatcgccgcc cccagcccct gatgctgttc tgccatcagg tgacggatat tgcctgcaaa
     9241 ttcatcatcc tgaatcagcg acaaatgggc gctgaggata gttttgcttt cgccgtcacg
     9301 ctcacgcaat tgctggttca gttgctcggc aagcgttgcc agactgtgct ccagtagggt
     9361 ggaatcttgc gcactcgcag ggatcacccg ataactgtcg aggctgtcgc tctgtaacag
     9421 ggtcagtgta cccacgccaa cgccgcttgc cagcacgttg ccgtacagta aatccgggtt
     9481 caggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgccagcg tcggctggac
     9541 gctatcgctg tcgataaagc gcacctggat atactcttcc agcactcgcc gcgcctgctc
     9601 ttcatcgcta ccgctaatgt tcaggctgca actgtcatta aataaagtgc cagtgccaat
     9661 cagcgccagc gagcttttcg catcggcctt tgcgttctgg cgatggttaa taaaagtgat
     9721 ttcgctttgc cactggctgc actgctcttt aagttcccac gccggacgag catgtagacc
     9781 gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt aatcaggaga
     9841 tcatctaaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa taagggcaga
     9901 taaaagttgc tccatctgca aaatgtcacg gcagtcggca atcgatgcgg cgcaatgtcc
     9961 gtggcgggtt gcaggcccca tcaccactgt gggtacgcca gtgccggtta aatgcaccgc
    10021 cccgccgtcc gttccgccgt tgctgaacat atctgactgc aacggtacgc caatttctgc
    10081 cgccacggtt tcgatccacg cggtaagttt tggcggcgca atcagcgact tgtcgcttaa
    10141 caccagcatc gggccgttac caatctggcg atggttagcc gcgccataat caaagttttt
    10201 cgcccagcag gcggtatcaa gcacaatggc gacatccggc gacaccgcgc gggtggcagt
    10261 ttgcccgccg cgtaagccaa cctcttcgct ggaacttgcc accagccaca cttccgcagg
    10321 cagctcggcg tcgtgcagct cacgcagtaa cgtcaccagc agatagcaac cgaggcggtc
    10381 atcaaaggct ttccccatca cccgctggtg agggagaacc tgaaaagtgg tatcgaaggt
    10441 gacgcgatcg cctggacgaa ttcctgcctg catcacttcg tcacaggagc gcgcgccaat
    10501 gtccacgcgc atggcgctga cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat
    10561 tttgcactct tcacgggtag tgatacgcac cgactgaagc tggcgggcag ccatgcgtac
    10621 attgccaacc ggcaacacat cgatcgcccc ttcgcgggag atgctgcgca ccataaatcc
    10681 cacttcgtcc atatgcgcac agatcatcac cttcggacct gtcgactcat tgagtcggat
    10741 cagcaccgat cccagcccat caaatcgcac ttctttttgc aggcgatccg cttcttcccg
    10801 caggatctgc cgtacttcct gttctgagga ggcgatcgca tctgcctcgc tcaacgcttt
    10861 taagagcgat aaatccatta tgcctctccc gtgagcaaca ctgttttcgg catggcgtag
    10921 agcacttctg cgccttgcgg ggtgaccagc acgacatctt caatgcgcac gcccccttgt
    10981 cctggcaaat aaatccctgg ctccacggtc agtaacatac ctggctgtag cgtcgtagtg
    11041 tcccgcggtg aaaaacgcgg ttcttcatgg acttcaatac cgatagcatg accggtgtta
    11101 tggccgaaaa acttgccata accggcctcg gtaatcacgc ggcgcgcggc atcgtcaacc
    11161 tgctggcagc gcacgccggg gcgaattaca gagattgccg cgagctgtgc ctgcaggaca
    11221 atctgataga cgttaaacag cgggtgagat tcggcgctca ccccttcgcc attcaccagc
    11281 aaggtgcgcg tcatatcaga gcagtagccc tgatacagcg caccgaaatc gagagtgaca
    11341 aactcgcccg ctgcaacaat cttgtcgctg gctttaccgt gcggcagcgc cccacgccag
    11401 ccactggcga caatggtatc gaaagaggct ttttctgcgc cctgccggcg cataaaccac
    11461 tccagttcag cggctatctc gcattcgctc atccccgcct gaataaagcg gcgaatatgc
    11521 tctgcgccac gatcggcaat cccgcaggca aggcggattt tctccacctc ttctggcgtt
    11581 ttgatttgcc gcagcacatc cggcgtggcg ctcaccagtt tcgcattgag ttcgaactgc
    11641 cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt ttgcaactgc
    11701 tcatcggcaa tgatttgtct gacgatggtg gcaagcgtgt gcatcgcgtc gagcaaatgc
    11761 agctggtaac cttgcgtgcg ggcttctaca tccgcgtaat agcgcgaatc caccagaatg
    11821 tgcgcacttt cacggctaat caccacataa cctgatccgg tggagatccc cagatgcggc
    11881 tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcgcctt aagccagtcg
    11941 cgcagcgaag cgagtaatgt catcaggatt gtccttctta caggctatcg attaacaatt
    12001 tgcctttacg gaacatcatc agacgcagga acaccaccat cagcgcagtt atgactgccc
    12061 ccagcgcgat ccccgccatg tagacgccga ggttagtgac cagcggccat gcccagatag
    12121 cggactccgg aaaccattgc actgcgccca gccagacagc ggcggttgag ccgacaatcg
    12181 cgccgaccat atacgacgga atggcggtga tagggctttc cagtgcaaac ggaatcgcac
    12241 cctcactgat ccccataaac gccaggaaca tcgcggtttt accctgcgga taaagctggg
    12301 cgttgaacaa gcgtttgcca gttaagcgac gatcgataat ggtcgccaga cccagaccaa
    12361 tcggcggaat aacgatagcg atagaacgtg cagtgaccgg taatacgtgg tcagtggtaa
    12421 agctgaaggc aacgaaacct gccgctttgt tgatcgggcc accaaggtcg attgctgtcg
    12481 ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcagcg gtcagcacgg
    12541 tacggatacc gccgttgatc cagccaccaa agggggtgat gacgtagtac atcgccagca
    12601 tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct aacaggaaat
    12661 ccggtaactg gattttctgg ttcatccact tcacgaggta accggcaacg atagaaataa
    12721 tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc atcgtgctgg
    12781 gatcgaagtt cagcagttgc gtcggctggg tagacatcaa gccgccgata aatccagccg
    12841 ggaacgccag tttgccaccg atagagttcg ccacaaaagc agcaaacatc ggaatggcaa
    12901 aaccaaacaa cacaccgccg aaggactgcg acagccaggc aaatttcagt aaggagaggt
    12961 cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatatca gcaggaattt
    13021 taagccagct ataagcaatc agctgactga aagcgaggat cacaccgccc ataattaagg
    13081 tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc cagaagctgg
    13141 atttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca tgcacaacgg
    13201 ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac tgttgttcag
    13261 aggcaatcat ctcttcgatt tctttgatga ttcctgcagc gtttttaatt gcgtcctgca
    13321 aagtgatttc ataaacgtcg cgtgattcga aacgctcgtt atcttccggg gtaactgcca
    13381 cggagtggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga ttctggatac
    13441 cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc gcttcttcca
    13501 gcgcctgagc ggccataaaa gtgtgagcca ggcccattgg gcaggcacat aaggcaatca
    13561 gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt
    13621 gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta atcccgctgt
    13681 acggaagtgt gacgcaggtc ggccttgtga ggggacacaa agaaaaattt aattaactgt
    13741 atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga tttagagaat
    13801 gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat tgtcatggac
    13861 gatgagatat accggaatat catggacata ttccttaaag cgccctttat cttcaaatgc
    13921 ggcgcggaaa ccagaggctt tgaagaactc aagaaagcgc ggcacgatac cacccgcaat
    13981 aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac ggcccataat
    14041 gacgcaaaac agcgacaacg cgcggcggca atcggtgcag ctgtcagcca gcgcgcgttc
    14101 ggtaatatct tttggcttga gattttctgg caggcggttg tcggctttaa caattgcgcg
    14161 atacaaattc accagcccag ggccagaaag cacgcgttcc gccgaaacat gaccaatttc
    14221 cgcacgcaat atttcgagga taatgccctc ttcttcacta ttcggcgcaa aatcaacgtg
    14281 accgccttct cctggcaagc ttacccaacg cttatcgaca tggaccagat gcgcaacccc
    14341 aagccctgtt ccggcaccgt aaaccgcaat aggcttacct tctaccggtt ctgcgccacc
    14401 aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag cagtaaaatc
    14461 gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa ttgagaacgc
    14521 ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga tggcaataca
    14581 gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg cttcgaggct
    14641 ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg caatatcaca
    14701 cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact ttgtcattct
    14761 tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc tgggggcata
    14821 acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca caattacttt
    14881 atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact aagtatctga
    14941 ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg ttattatcgc
    15001 tccccgccgt ggcaattggg attgcgtcca gtcttattct gattatggtg atgaaaatcg
    15061 cctcggtatt acagaatttg ctctggcaac gactaccggg aactctgggg atagcccagg
    15121 attcacctct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg gggttggtta
    15181 tccgtttcag ccagggtcat gccggaccag atcccgcctg tgaaccgctt atcggcgcac
    15241 cggttccgcc ctctgcgcta ccaggactta tcgtagcatt aattctcggt cttgctggcg
    15301 gcgtcagcct ggggccggaa catcctatca taaccgtcaa tatcgccctt gccgtcgcca
    15361 ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta gcctctgccg
    15421 gaaccatcgg cgcgctgttt ggcacacctg ttgcggcggc attgatattt tcgcaaacct
    15481 taaatggcag taatgaagtt ccgctatggg atcgtctctt tgcgccgtta atggcggcag
    15541 cagctggtgc acttactacc ggattatttt tccatcctca tttttcactg cccattgcgc
    15601 attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg gcgattacca
    15661 tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgcgatg atgcatcaaa
    15721 tgaaaaatcc ggtgttcgtg ctgggtattg gcggatttat tctcggtatt ctgggggtta
    15781 ttggtggacc ggtttcgctg tttaaagggc tggatgagat gcagcagatg gtggcaaatc
    15841 aggctttcag caccagcgat tactttttgc tggcggtgat aaaacttgcc gccctggtcg
    15901 ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcattgttt gtcggcgtgg
    15961 cattagggtt gatgcttcat gagcacgttc ccgccgttcc agcggcaata accgtttcct
    16021 gcgctatact cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg
    16081 cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc atgctcccgg
    16141 catggctgtt attagcaggt aagccgatga tgatggtcaa ccgtccgaag caacagccac
    16201 cccacgataa cgtttagcaa aaaatgctct cctttatgat ttaagagtta tggcgatgat
    16261 acggcgcttt acctccccgt aatattgcgt taacaggccg ctgacggctt cgatcgtgaa
    16321 ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca tttaccccgc
    16381 ttgcggcaaa cgcaggtgaa atcaccctac tgccatcaat caaattacaa attggcgatc
    16441 gcgatcatta cggtaattac tgggacggtg gtcactggcg cgaccgtgac tactggcacc
    16501 gcaattatga atggcgtaaa aaccgctggt ggcgtcatga taatggctac catcgtggct
    16561 gggataagcg taaagcgtat gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc
    16621 atcgtggaaa aggccgcgga catggacacc gccattaaac gcatccgtca atggagcaca
    16681 atgcctgatg cgacgctagc gcgtcttatc aggcctacaa accagctctt taacgcgtag
    16741 ggcggataaa gcatttacgc cgcatccgcc aatggtgcca aatgcgacgc tcattcaact
    16801 acaatcccag tgccgttcct accagtaacc agatattcag cgccactacc agcaccacaa
    16861 tcacccagcc tgtctgtttt acgcgtttac tgttcaccag atcgcccatc aacttgctgt
    16921 cactggtgaa aatcaacagt ggaaccagcg ccagggcgat accaaaactt aacagcacct
    16981 gactcataac cagaatccgt gtcggatcta atcccatcag aatgacgata aatgacggca
    17041 gcatggtgac tgtacgacgc acccacagcg ggatatgaaa gcgaatgaag ccctgcatca
    17101 ctacctgccc cgccagcgtc cccaccaccg ttgaagacag tccggcagca accaggctta
    17161 atccaaagac cgttgccgca gcatggctta acagcggttg cagcgtcaga taagcctcat
    17221 caagatcggc aacaccagta tgaccagaaa agtggaacgc cgccgcagct gtagccatca
    17281 tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc cacatcccat ttggtggcgg
    17341 aataacgttg ttgacgcgaa ccgccatgta aatgctgagt gagcgaagag tgcaaataaa
    17401 tcacatgcgg cataatcgtc gcccctaaca cgcccgctgc caggaagacc gcctccgaag
    17461 taggtaaact cgggatcacc attcctttac ccagctgcgc caggttaggc tgggagaaaa
    17521 tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa cccgccaatc actttctcca
    17581 gcggtttttg cccgcgacgt tgcagcatta aaataaggaa agtcgcgatc cccgtcagca
    17641 ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa accgatcgcc gcaccaataa
    17701 attccgccag gtcggttgcc atcgcaataa tttctgcctg aacccaatag aaccacacga
    17761 cgggacgcgg gtagtgatcg cgaatctgct ccgccagatt tttaccggtg gcaatcccca
    17821 gtttggccga gaggatttga atcagcatcg ccatcaggtt agcccaaacg acaacccaca
    17881 ataactgata gccgaagctg gcacccgcct gaatattggt cgcaaagtta ccgggatcga
    17941 tataaccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc ttgcgcgccg
    18001 cccgtccgct gctactctca acgcgatagt tcgtcatctt gtgcctctaa aacatagcct
    18061 ttgctatgtt ttatgctacg ttaaatgaga atgattatca aattcattta aatgggttgt
    18121 gatgatttct ctgatagacc aggattatga ctacgaaaag atagatgact cgaatgttat
    18181 ggttatgttt aatattagca catttacata accttccgct tccatacaca acatagcaga
    18241 aatgtatgac agatcactat ttttgaagct tgtcacagga cgtcattata gtgtgtgtca
    18301 gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac ctgcctcata
    18361 tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt tgttgcgatt
    18421 ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt tattcaactg
    18481 cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg tctgggcttc
    18541 gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggtt ttgccaacga agggactaac
    18601 ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa agtgctgtgc
    18661 ccaatcgtct ttatctctgc gctgatcggt attctccagc atattcgcgt attgccggtg
    18721 attatccgcg caattggttt cctgctctcc aaagtcaacg gcatgggcaa actggaatcc
    18781 tttaacgccg tcagctccct gattctgggt cagtctgaaa actttattgc ctataaagat
    18841 atcctcggca aaatctcccg caatcgtatg tacaccatgg cagcaacggc gatgtccacc
    18901 gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg agccgaaata cgtcgttgcg
    18961 gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa cccttaccgt
    19021 gttgatgcca gtgaagaaaa tattcagatg tccaacctgc acgaaggtca gagcttcttc
    19081 gaaatgctgg gtgaatacat tctggcaggt ttcaaggttg ccattatcgt tgccgcgatg
    19141 ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac tgtttgccac cgtgactggc
    19201 tggtttggct acagcatctc cttccagggc atcctgggct acatcttcta tccgattgca
    19261 tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat ggcgaccaaa
    19321 ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc cacgctctct
    19381 ccgcgtgcgg aaggcatcat ctctgtgttc ctggtttcct tcgctaactt ctcttcaatc
    19441 gggattatcg cgggtgcggt taaaggcctg aatgaagagc aaggtaacgt ggtttctcgc
    19501 ttcggtctga aactggttta cggctctacc ctggtgagtg tgctgtctgc gtcaatcgca
    19561 gcactggtgc tgtaagacca tacataaaaa agccggggat aattccccgg cttttttacg
    19621 cctgttaatc agctaatggc tgcggacgac caattaaata tccttgcaga tattgcaccc
    19681 caagtttatg caatagcgcc tgctgctgtg gcgtctcgac aaactccgcg accacactca
    19741 atgacttcgc tttcgccaga tcggtaattg atctcacaat catcgcatcc agcgtgttcg
    19801 tgacgatatc tttcacaaag acgccatcaa ttttgatgat atcagcctgc aaacgcttta
    19861 accgttcgta gttggcatat ccggtgccaa agtcatcaat cgcaatccgg aaaccaaact
    19921 tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt agaaaacgcc tgctcctcgg
    19981 tgatctcaag aatgaccgcc tgcggggaga tgtaataacg cttaaacaga cgaataatcc
    20041 gcccggcaat attcttttgc agcagcgtga gcggcattaa attgactgaa aagcgtggac
    20101 cttttttgtc gcaagggtgt gttgccagcc acttcaacaa ggattccagc acttgcaaat
    20161 caaaacgcgc gctaaggtta aactgagcaa taaggggcag aaacttatcc ggggtcataa
    20221 tgccgccgtc atacttcagt cgcgcgagga tctcatcata accttcaccc tctttgttgc
    20281 gaattggctg ggcgtagagc agcaaatcac cctgatctaa cgccgtgcga atggtattca
    20341 gcaatagcac ctgtttagtg gtttgcccgg aaaccatctc ctctctgcta tccagcgcca
    20401 gcacatgatg atgtgcgcag gattgctccg ccagccagct taactgcccc aacaagggtt
    20461 gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc accatagccc atatccagcc
    20521 cggtattgtt ccagtgaatt tgccgactat tcaggatgtt aaccatatgc tggagtcgcc
    20581 cttccgtttc cggcccgctc agcactaaca gcagttcact acccggcaat tgatacaact
    20641 tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat tgagcggata cagtgaacgc
    20701 gcattattaa gccgtaatga cgactcataa actcaagatt atcaatgcgc aggcagcaaa
    20761 aactcttgcc cgcctcttgc tccggcgctt gctccaacgc acgaaagttg ggtagaagcg
    20821 ttaacggatc ggtcagcgcc tgcaaatgcc agcggcggtt aagccattca ctacgatgat
    20881 aaatacgcac catatagagc aggcaaacac taaaggaaat cagtactgcg agaataaatg
    20941 ccagcgaata ttcggttagt accccttgca aaaagttctg gttgtaattc agaaggcaaa
    21001 gcgttgaaac agcccaggtg agatttaaaa acggatagcg aatcttaccg acaccgaggg
    21061 taaagatgat aaagaaaaca ggtaccaggt aaccggcaat aaagtcattt tcatacggtg
    21121 tgcacatcag aagtagcagc acgctaagag ctgccagcca gcttaaggta aatgcgcgtt
    21181 tctctttgct caacgacgga gcgatatccc tacgccacaa tatctgtgca aagtgggaac
    21241 ttacaatcat gcgggtgaga tagtaaaaaa gcatgttgta aatcagcact gcggtgaaaa
    21301 ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtagatatct
    21361 ttagcggaaa atcaaagaaa tttcccacaa gatacatgct gcatttgatg ccaatcggcg
    21421 ccaccaaacc aagccagaac agacgttgcc agacgtatcg gctggtcaat ccataacgcc
    21481 aacgcgtccc caattgccag cgtaatattg cacaggcaca caggactgca aacgcctggc
    21541 tgccgatcat gacggcggtc tgtaaaaaag agagatgcag gttccacaga ttggtgcaaa
    21601 acatcccgca taaaatggga acgacaccac gccagccaaa gataaacaag attgacagca
    21661 ttacgcacag tggcatccac gccagaaaga tataattgga atggataaca gcaagtggcg
    21721 aaattaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    21781 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    21841 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    21901 tgctgttttt tcagtcagtg atgcattcac cacacggcac tgccaggata tagactgtat
    21961 tacaaaagcg gcaaaaggca gagacaaaaa acccccgctt tgcagcgagg gtttgaaatt
    22021 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    22081 gctgagctat agccccacga gtcgtttacg taccaaattt actgggtgca aaatttggtg
    22141 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    22201 gctatagccc catcacgtaa agcttgtcga gttgacgggc ggcatcatat gaattccgcc
    22261 cgaatgtgtc aacggcaaat tgccacgtgt agtttcaatc gctgaaaaat caggcaaatg
    22321 aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt cctgtaaagt
    22381 ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt tatgttcaag
    22441 gagaggatga cgccagatga acttgccaga ctgaccggct atagccgcca gaccattaat
    22501 aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac caggcgtcca gggtggcaaa
    22561 gctcgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc tgaacgtcca
    22621 gaaggtcagg gagaagcgcc agccctttct ggtgatgcgc cgcttgaagt gttgctggtg
    22681 acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt gcttctgcgg
    22741 gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata atatgaaaag
    22801 attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggcgtgg ctaaacaaac
    22861 tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa aaatttcccg gcgtgaaagg
    22921 cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc agaacacgcc
    22981 agccttccat aacacaccga tgcttctgga agccgaagaa ccccttgccg aatacgcacc
    23041 gggtattcgc actcctgctt atcggcaaat catcagcgca atagacaaca tgactcacag
    23101 tgagcaagaa aaagttgcgc aatttttgtc acgcgaagga attcgtaatt ttcttgctcg
    23161 tctcgacata gatgaatcag cataaataaa aaacggcagg ggtatctctc ctgccgttta
    23221 tcttttatgc gctaattact gctgattttc gcgttcagca ataaaagcca gcgctttgtt
    23281 gatacgctcg atactgcggg tcttaccgat tgcgtgaacg gtaacatcca gcgctggaga
    23341 ctgccccgca ccggtaacgg cgacacgcag cggcatacca actttaccca tacccacttc
    23401 cagctcatcc gccgtcgcct gaatagcgtg atgaacgttt tcagcggtcc agtcagtaat
    23461 cgcagtcagt ttgtcacgaa ccacttccag cggctgacgc gctaccggac gcagatgttt
    23521 tttcgcggcg tcggcatcga actcagcaaa atcttcgtag aaataacggc agctctgtgc
    23581 catctctttc agcgtcttgc agcgttcgcc cagcagtttc accagatcag ccagctgcgg
    23641 gccgttacgg gtatcgatat tttcctgctc aatgtgccac tgtaagtgag tagcaacata
    23701 ctccggcggc aacgcgttaa tgtagtgatg gttcagccac agcagcttgt cggtgttgaa
    23761 cgcactggca gatttgctga cggcattcag agtgaagtat ttaatcatct cttcacgagt
    23821 gaagatttcc tgatcgccgt gggaccagcc cagacgcacc agatagttca gcagtgcttc
    23881 tggcagataa ccatcatcac gatactgcat tacgctgacc gccccgtgac gtttggacag
    23941 ttttttaccg tcatcgccgt tgatcataga aacgtgcgcg taaaccggta ccggcgcttt
    24001 cagagcctta agaatgttaa tctggcgcgg tgtgttgttg atatggtctt cgccacggat
    24061 aacgtgggtg atttccatat cccagtcatc gacaaccaca cagaagttat aggttgggga
    24121 accatcggta cggcggataa tcagatcatc cagctcctgg ttgctgaact cgatcggacc
    24181 acggatctga tcgtcaaaaa caacagaacc ttcctgcggg ttagcaaaac gtacgacaca
    24241 cggctcatca tcagcgtgat gctcatgact gtggcggcag cgaccgtcat aacgcggctt
    24301 ctcacctttc gccatttgct cttcgcgcag cgcttccagg cgctctttag agcaatagca
    24361 tttataagct gtgccctctt ccagcatctg atcgatcacc gcgttgtagc gatcaaaacg
    24421 tttggtctgg tagtacggac cttcatccca ctccaggctc agccagttca tgccatccat
    24481 aatggcttcg atagcttccg gcgtggaacg ctcaagatcg gtgtcttcaa tacgcagcac
    24541 gaactcaccg ccgtggttac gtgcaaaaag ccaggagtaa agagcagtac gcgcgccgcc
    24601 aacgtgcaga tagcctgttg ggcttggcgc gaagcgagtt ttgattttca tgaaatggcc
    24661 ttacgtttag aaagatgccg acaaccggca aatcctggaa aaattgagtg ggcgatattc
    24721 tatcactccc gcctgattcc tcaatgtagt tcgggttgca accttacgca ttattgtttt
    24781 cgttgacaaa ttgcgcaaca cggtctgttt tgcgatcatc ttgcttaaat ttacgacgaa
    24841 cgaacaattt ctttagaaaa tgcgttgact cattttgaac tctccctata atgcgactcc
    24901 acacagcggg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    24961 ggttcgatcc cgtcatcacc caccaactac tttatgtagt ctccgccgtg tagcaagaaa
    25021 ttgagaagtg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    25081 ggttcgatcc cgtcatcacc caccactttc tcgccagcta gatttcttgt aacaatgtga
    25141 agtaccgaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    25201 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    25261 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    25321 ccctaaaggc gcttttttgc tatctgcaat ttgcgaaatt gcctgatgcg cttcgcttat
    25381 cagaccaact atgtccggca gttcccgtgc ctctcatcta ctgcgccaac gccgccagca
    25441 gtttaaccaa cgcgggcgtc acctgctgcg tttcataaac aatatataag tctgccggga
    25501 tgcgttcttt gagcggacgg aatatgacac cgggccagtt catctgcgcg tagctgtccg
    25561 ctatcaatgt gataccaata cccatactga ccatggcgag taccgtttgc ggttcattaa
    25621 cttcgcgaat aacaaccggt gaaaatccca cctgctggca aactcgctgc aaaaaatccc
    25681 agtcagtgta cacgggcggc attgtaacga aatactcgtc acgtagcgct tccagcggga
    25741 cggtggaaaa tgatgagaga tgatgctctt caggcatcgc caccagaaac gccgattcat
    25801 gcaaccgtaa gctggtaaaa ccagtcggtg gttctgtcgc cattcgccag atcccggcat
    25861 caagttcgcg gcgttccagc aaggccattt gcatcgctgg catcttttcg cgaaacagaa
    25921 cttcaacgtt aggattttct ctaaggaatc gccgcataac cgggcgcatc cgtccccaca
    25981 ttgccgttcc cacaaccccg agttcaatcc gccctgcttc tccccgacct atttgttcaa
    26041 cccgagccaa tacattattt gcattcacca gcaatcgacg cgattcttcc atcaagattt
    26101 tgcccgcgtg tgtcagtacg acgctgcgcg aatggcgaat aaaaagctgc gtgccgagtt
    26161 gattttccag ctctttaata tgaatactga gcggaggctg agacatattt aaacgcgctg
    26221 ctgcgcgacc aaaatgcaac tcttccgcta cggcaagaaa ataacggagc aacttaagat
    26281 ctgttctata tacgcgttcc ataattaagt gctccatata aaaaatatat cgtattaaat
    26341 tatcatggca taaagtattt caccaccaga tatccgacat acataaacac gatggataat
    26401 atacccggca atacatttgg aacaggaata ggtaatcgca cgattgtaaa caaagcacca
    26461 atgccaaaac caacgccaga agaaaataaa atatctttca ctaattaacc tttatcataa
    26521 aagcagcccc aaggaacaga gctgcgaatc cttttaatgt gtaactgaac gatgcgttat
    26581 cttttcaggg tcatggttat atttaaaccc aaagaaaaat atcactgcga ggaaaagagc
    26641 ataccctgca aacatcagcc aaatggtatg ccaatctttt acgccatcca ccgagaagta
    26701 atccactgcc ataccgctca gaatcgagcc aacccatgcg ccgacgccat ttaccatcgt
    26761 cataaaaagg ccctgggcgc tggcacgaat gctggaatca acttcctgtt cgacaaagac
    26821 cgaaccagaa atattgaaga aatcgaatgc acaaccgtaa acaatcatcg acagcaacag
    26881 caaaataaac ccggttggtg atggatcgcc ataggcaaag aagccaaagc gcagcgtcca
    26941 cgccaccata ctcatcagca tgacggtttt aataccgaaa cgcttcaaaa agaacggtat
    27001 cgtcagaatg aaaccgactt cagccatctg tgaaactgac agtaaaatgg acgggtattt
    27061 caccacgaag ctatcagcaa actcaggatt acgggcgaaa tcatgcagga atgggttgcc
    27121 aaaaacgttg gtaatttgca gcaccgcgcc cagcatcatg gcgaagagga aaaagatagc
    27181 catgcgtgga tttttaaaca gcacgaaggc atccagaccc agcttgctgg caagcgatgt
    27241 agtcgctttt ttctccgcaa ccggaatctt cggcaaagtc aacgcataag ccgacagcag
    27301 caatgacgca ccggacgcga tatacagttg cagactactc aattccagat gcagcaggct
    27361 tactgcccac atggcgacaa tgaaccccac cgtaccaaaa acgcgaatgg gcgggaaagc
    27421 ggtcaccggg tcaagccctg cctgggcaag acaggaataa gagacgctgt tcgataacgc
    27481 aatagtcggc ataaacgcca tcgcattgac taacatcacc caaaacatca tatccggatc
    27541 agttacggat gccgcataaa aaagtacgcc cgcacacacc aggtggcaca gcatgtatgc
    27601 acgttccgca cgcagccatt tgtctgcgat gatccccatt atacccggca taataatcgc
    27661 ggcgatccct ttggaactgt aaaccatgcc aacattagcg ccggtgaaat gaagggtatt
    27721 aatcatgtaa gaaccgaggg taaccagcca gctcccccag ataaaatatt gcaaaaagga
    27781 cattaccttt aagcgcaaag cgatgctcat aatcgtttcc ttgtcgcatc atatggcctc
    27841 actcagtaag gccgtttatt gttgttttac tcaggcaatt ttgcgcagaa agccgcaaat
    27901 aagattaata aagttttgct ttgagagttc cgctgctgcc agcgtttggg catgagaaag
    27961 cttcacgtca ctcagacctt ccgccatgtt ggtaatcgca gagaccgcaa cgactttaag
    28021 ttcgcaatgg cgagctgaaa taacctcagg caccacagac ataccaacaa catccccacc
    28081 aataatttgc atcatgcgaa tttccgccgc agtctcgaaa ttcggccccg gatacgagac
    28141 gaacacgccc tccgtcagag ggaacccctc ttctttcgcc actttttgta acagtgcgcg
    28201 gtattccgca tcgtaggcat tcgccagcga gaagaagcgc tcaccaaaac gttcatcatt
    28261 aagacccacc atcggcgttc ccggcatggt gttgatgtga tctttcaatg cgaccagact
    28321 gccggccccc acttcagggc gcagtgagcc agccgcattg gtgcagaaca gcaactcgca
    28381 gcccagcaac ttaaaggtac ggattgcatc cgtcatgatg gtcatcccac gaccttcgta
    28441 gaaatgtccg cgacctttca tacacgccac tggccccccc tggagatacc ccagcaccag
    28501 ctcacccgca tgaccgtgta cggtacttac cgggaaccca ggcagctttt cgtaggaaat
    28561 tgcgaccgcg ttctcaatct gatcggccag cgcgcccagc ccggaaccta aaataaaggc
    28621 cactcgtggc gtgaaatcag gtttataagt cttgataata tctacgcaat acagtgggtt
    28681 atgagaaaac tgagcctgat acatatatat cctttttttg tagggtggaa tctaacgcct
    28741 ttttataacg agattgccct acctcatacc aatacacttt tttcatgtat ccgatagtta
    28801 ttcgatatcg atatatttat attttttcac tattaatagg ctgaaaaaac tgtattggta
    28861 ctctcctttc ccttgcatta acaatatgcc gcttttaatc aaccttttat taagcaatca
    28921 actaataacg atcggcatag ggaattatgc tatgaaaaaa catcttttta ctctgacact
    28981 ctcttctgta ttggcgatac cagcagtatc ccatgcagaa tttaaaggcg gttttgctga
    29041 tataggtatt cattatcttg actggactag cagaaccact gagaaatcgt cgaccaaatc
    29101 acacaaagat gattttggat atctggaact ggaaggcggc gctaacttta gttggggaga
    29161 aatgtacggg ttcttcgact gggaaaactt ttataatgat cgtcatgata aaccgggtag
    29221 cgagcagcgt tatacattta aaaataccaa ccgtatttac ctgggtgaca ccggatttaa
    29281 tctctacttg catgcgtatg gcacctacgg ttctgcgaat cgtgtgaatt tccatgacga
    29341 tatgttcctt tacgggatcg gctacaattt caccggcagc ggttggtggt tcaaaccgtt
    29401 ctttgcaaaa cgctatacag accaaaccta ttataccggc gataacggtt atgtcgccgg
    29461 ttgggttgca ggttacagct ttatgctggg cagtgagaaa ttcaccctca ccaactggaa
    29521 cgagtacgag tttgatcgtg atgccaccta cgcggcaggt aatggcggca aagaaggtct
    29581 gaacggtgcc gtcgcacttt ggtggaatgc aacttcacac atcaccacag ggattcagta
    29641 tcgttatgcg gatgacaaac tgggcgaaga tttttaccag gatgcgataa tctactccat
    29701 caaatttaat ttctgattga ttactgccgg agctcccggc agtatgaatt cactctatat
    29761 ctgatacaac ggatcccctt cccgcccagc cattagcgca ttgccctggt ctcgtactac
    29821 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    29881 ctggcgttca accactggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
    29941 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    30001 cgccggatga ccaatctcct gcacaatatt cgcctgaata ccgttgcgcg ccagcgctgc
    30061 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    30121 tgcctgccag gggacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    30181 gaaaggctcg gaaagaatca cttcacattg cagatcgccc actgggccag gatcgatgac
    30241 gatgccaaaa tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    30301 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    30361 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    30421 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    30481 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    30541 aacaccagta tgattttcca gctccttcac actgtgactc actgccgact ggctcaggcc
    30601 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    30661 ttgctttaaa gaataattca tctgtttttt tcatgaatgg atgcaataaa tcaattttat
    30721 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    30781 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcatctttct
    30841 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccactgca gcgattgctc
    30901 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    30961 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggcg
    31021 tgctgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    31081 acttgtgcat tctccccgcg accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    31141 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    31201 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
    31261 tcggtaaaat tatgctgcaa ctgttgctgc catttgtgtt ggggcatctt tcccggcctt
    31321 ggattggtga ctgggtgtcg cgtaataaaa aatggattgc gaaaactgac cagacgtcca
    31381 ttctgttggt ggtttatacg gcattcagcg aagccgtcgt taacggtatc tggcacaaag
    31441 ttggctgggg atcattgctg tttatcgtgg tggtcagttg cgttcttctg gctatcgtga
    31501 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
    31561 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    31621 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    31681 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    31741 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    31801 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    31861 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
    31921 gccctttgta cttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    31981 ccatctcagc tacccagcaa acgcagcatt tccgtttcgt cgatgacttc aatgcccagt
    32041 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    32101 ttcgacacgc tgcccgcgac tttcgccccc agttcgacca gtcgagcttt agcgtcatca
    32161 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cggactgtca
    32221 atctcttccg cattgataac gatcggctca ggccagtgaa caccttccgc caacagctcg
    32281 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga ggcaacgaca
    32341 attccaacat caggcacctt ttgcagctct tcaatcgacg cggcttccag cgcttccagc
    32401 gtgccgaaat atgccgccag acctgctgcg gtagcctcgc caacttcacg gataccaagt
    32461 gcatagagga agcgggcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    32521 tgtgccgatt tcggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    32581 agatccgccg gagtgtggac atattctttt tcaaccagtt gatcgatgat tttgtcgccc
    32641 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagagactc tttacgctgc
    32701 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcgacg
    32761 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    32821 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aataaccact
    32881 ttatcgccaa tgcgtaaacc aagacgttcg atttcgtcag cattatgcag tgtagcgtta
    32941 ctcaccaaca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    33001 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgttcctg cgccgggaat
    33061 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgtgcc
    33121 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg acggtcttct
    33181 tctactttgt ggtagaacgc cagcacttct tccgccgatt cacaaaacgt tacccgatcg
    33241 ctgaccggca aaccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    33301 tcgccacctt ccagaacacc gacgccatag cagaagaaag tgagcggtcg cttcgctgta
    33361 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaacact
    33421 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    33481 acttcaccac gcacttccag acgcgccggg atattctctc cgtgtagctt cagcggaatg
    33541 gcgcgaatag tacgcacatt agacgtaata tcttccccgg tggtgccatc gccacgcgtc
    33601 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaagacc atccagcttc
    33661 agctcacagc accaggtgac cttctcgttg cttttcagac ggtcctgcac tcgtttgttg
    33721 aaggcaagaa agctttcttc atcaaaaacg ttatccagcg acagcattgg cacttcatgg
    33781 cggatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    33841 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    33901 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
    33961 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    34021 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    34081 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    34141 acggcgctga tcgtcaagca ctacaccgcc cacttcatcg gcaatatgct gcgcagattg
    34201 cagcatcagc ttaaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    34261 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    34321 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatgtt
    34381 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttaa
    34441 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcctctttgc gcttcggttt
    34501 atccgtaact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    34561 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc actggctgcg gcgctgacgc
    34621 gacctgtggc gaaacaggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    34681 ctgcacaggt tgctgcaccg gctgcgttgg acgcggagca tgttgcggcg gtacctgcgc
    34741 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg
    34801 gtgttgcggc gacggacgag caacctcatg ctcctgggcg ttagccgggg catgattcac
    34861 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
    34921 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
    34981 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
    35041 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
    35101 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
    35161 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
    35221 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca
    35281 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
    35341 ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt
    35401 accgctgctg gtcaatattt ttttgatggg gggcgcattc tggtggctct ttacgcaact
    35461 cgatgtctgg atcccgactc tcatgagtta tgttccggac tggttacaat ggctgagcta
    35521 tctgttgtgg cccctggctg tcatctctgt gctgttagtg tttggctatt tcttctcaac
    35581 gattgctaac tggattgctg ctccgttcaa cggtttattg gctgaacaac tggaagcaag
    35641 actgactggc gctacaccgc cagataccgg aattttcggt atcatgaaag atgtgccgcg
    35701 aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct
    35761 aattctttac ttcatccctg gcattgggca aaccgtcgcg ccggtactgt ggttcctgtt
    35821 tagcgcctgg atgttagcca tccagtattg tgattacccc ttcgataacc acaaagtgcc
    35881 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
    35941 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcattatgc ccgttgccgt
    36001 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcca tgtggcggta
    36061 acaatctacc ggttattttg taaaccgttt gtgtgaaaca gggggggctt ataccgcccc
    36121 ttattccatc ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt
    36181 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
    36241 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
    36301 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
    36361 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
    36421 tgaaaccagg cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct
    36481 atgtagctgc cgctcgcggt tacaaactta ccctgaccat gccagaaacc atgagtattg
    36541 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag
    36601 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc
    36661 tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc
    36721 cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
    36781 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
    36841 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag
    36901 agattaaacc tggtccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
    36961 ttgatctcaa gctggtcgac aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
    37021 cacgtcgtct gatggaagaa gaaggtattc ttgctggtat ctcttctgga gcagctgttg
    37081 ctgcggcgtt gaaactacaa gaagatgaaa gctttaccaa taagaatatt gtggttattc
    37141 tgccgtcatc gggtgagcgt tatttaagca ctgcattgtt tgccgatctt ttcactgaga
    37201 aagaactgca acagtaatgc cagcttgtaa aaaatgcgta aaaaagcacc tttttaggtg
    37261 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
    37321 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
    37381 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac
    37441 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattacgct cttcgtgcgt
    37501 cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
    37561 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
    37621 cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
    37681 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
    37741 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
    37801 cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
    37861 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
    37921 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
    37981 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
    38041 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
    38101 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
    38161 caggaaatca tagccctgat taaagataag cacatgacag ctgacgcagc tgctcatgaa
    38221 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
    38281 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
    38341 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
    38401 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
    38461 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
    38521 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
    38581 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg
    38641 gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt
    38701 caccaggtag aagtgtgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
    38761 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg
    38821 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctct
    38881 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
    38941 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
    39001 aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctctgcttt cggtaaattg
    39061 cgcatcatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
    39121 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
    39181 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
    39241 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
    39301 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    39361 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    39421 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    39481 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    39541 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    39601 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    39661 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga
    39721 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    39781 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggc attgctatca aaccaacggg
    39841 taacaaaatg gtcgcgcctg tagacggcac cattggtaaa atctttgaaa ccaaccacgc
    39901 gttctctatc gaatctgata gcggcgttga gctgttcgtc cacttcggta tcgacaccgt
    39961 tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgtg tgaaagttgg
    40021 tgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac
    40081 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt
    40141 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    40201 ggcgcccatc ggcgccattt ttttatgctt cagccagagg cggcaaaatc aattcatcgc
    40261 tctcatgctg ctgggtatag cgcatcactt ccagtacgcg caatcccgcg cggtgtaccg
    40321 catcggttaa cgccttccct ttcagcaatc cactgataag ctgagcacaa aacaggtcgc
    40381 cagtcccttt caggtcagtt ttaacccggg aatgggaaat gacattcacg ctgtcggcac
    40441 tgaccaccac cacctgcatc tcctgatttt cttcattgcc ggaggcgctg gtaatcacca
    40501 cccactttaa tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc
    40561 ggcaatcttt accggtcagg atttccagct caaagatatt gggggtaatt ccctgtgcca
    40621 gcggcagtaa atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat
    40681 caatatcgcc aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg
    40741 cagtcagcca ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta
    40801 cagcacgaag ttggcgcagc gcatcacgct cctgaagcgc acgcaaataa ccgctaaacc
    40861 attcatccgg aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata
    40921 ccgtcggcac ggcaaagaca ttcaggccgt tctgctttat agcaggcacc gcaatgctgt
    40981 tgcctacgct gccgtacact acctgcgact gcacggcgac gatatccgcc tgcagtgccc
    41041 tgctcttatc gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc
    41101 ctcatcgtat tccagccgaa actttacctg attctggcag tcaaatcggc tattacaaaa
    41161 caaggataag gtaattcagt gaagaaaatc atttgtctgg tcgttacgct gctaatgaca
    41221 ctccccgctt atgcgaagtt aacggcccat gaagaagccc gtatcaacgc catgctggag
    41281 ggattagcac agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat
    41341 gaagcggttt ctcatctgcg cctgaagctg ggcaataccc gtaatcgcat tgatacagcc
    41401 gagcagttta ttgacaaggt ggcctcgtcg tcatcgatta ccgggaagcc gtatatcgtg
    41461 aagatgcccg gtaagagcga tgagaacgca cagccttttt tacatgcgtt aattgcgcag
    41521 acggataaaa cggtgcctgc gcaataatct taaatccccg ccccctggct aaaatgctct
    41581 tccccaaaca ccccggtaga aaggtagcga tcgccacgat cgcagatgat cgccaccacc
    41641 accgcgccag ggttagcttt tgccacccgc agtgctccgg caaccgcgcc gccggagctt
    41701 acgccgcaga aaatcccttc ccgcactgcc agttcgcgca tggtgttttc cgcatcgcgc
    41761 tgatgaatat ccagcacctc atccaccaga gaagcattga aaatccccgg cagatattcc
    41821 gcaggccagc ggcgaatgcc gggaatactg ctgccttctt ccggctgcag gccaacaatg
    41881 gtcaccggtt tggattgttc gcgcataaag cgtgacacgc cggtgatagt gccggttgtt
    41941 cccatgctgg agacaaaatg ggtaatacgc ccgccagttt gctgccagat ttccggcccg
    42001 gtagtggtgt aatgggcgta agggttatcg ggattattga actgatcgag caactttcct
    42061 tcgccgcaat tcgccatctc cagcgccaga tcgcgtgcgc cttccatgcc ctgttctttg
    42121 gtgaccagaa tcagttctgc accgtaggca cgcatcgccg cacggcgttc ctggctcatg
    42181 ttgtcgggca tcagcaattt catgcgatag cctttaagcg ccgcaatcat tgccagcgca
    42241 atgccggtgt taccactggt ggcttcgatc agcacatcac ccggtttaat ctcgccgcgc
    42301 ttttccgcct cgacaatcat cgaaagcgcc gcacggtctt ttaccgatcc cgccgggtta
    42361 ttgccttcca gttttaacca cacttcactg ccgttatccg gcgccattcg ctgcaacttc
    42421 accagaggcg tattgcctat tgtttgttct aatgtactca cgatctctgt ccatacgtgg
    42481 tgttgcctga tgcgacgctt acgcgtctta tcagacctac aggttacaaa accttgccat
    42541 taaaaaaccc ggatcgcggg aagcgcctcc gggcgtttaa cattcactca acctatcagg
    42601 cgctttgtgc gagagcaagt tcctcatcgc gggtttcaat acgctcatcg ccgttataca
    42661 gccgcgcatg ttgcagacca acgtataaac gatcgccacg ctgcggggca tcgtcgccat
    42721 gcatcacgac cgtcagcggt tcgttgtacc accccagcgg ctgcaccact aattgggtgt
    42781 agtgaccttt cgggctggct tccagtacct gtacgggtag cggcgaatcg aggctggtac
    42841 ggcggctgat atccacttcc cacgggcgca ggaagagatc caccggcccc tgatacgcgg
    42901 gggtgtatcc cagcggccag cgatgtgcgc caacgtggaa ctgcccgccg cgaatggttc
    42961 cctgcaggcg gtttacctcg cccataaact ccagcacaaa acgagtcgcc ggttcacgcc
    43021 atacctgatc cggtgcgtca gcttgttcaa tattgccctg actcatcacc actacacgat
    43081 cagctacttc ggtcgcctct tcctggtcgt gggtcacgaa cacgctggtg aatttcagtt
    43141 cttcatgcag ttgacgcagc cagcgacgca gctctttacg cacctgcgca tccagcgcgc
    43201 caaacggttc atcaagcagc aaaatttgcg gttccacagc cagcgcgcgt gcaagcgcca
    43261 cacgctgttt ctgaccgccg gaaagctgcg ccggataacg atccgccaga tgagcaagct
    43321 gcaccatctc cagcaatttt gtcactttcg ctttgatcgc cgccgcattc gggcgttcac
    43381 gccgtggcaa caccgtcagg ccaaaagcga tattgtcaaa caccgtcata tggcgaaaca
    43441 gcgcgtaatg ctggaacacg aaaccgactt tacggtcccg cgcgtgcaga cggctcacgt
    43501 cggtaccgtg aaagcgaata tgcccactgg tttgatgctc cagcccggcg ataatgcgca
    43561 gcagcgtagt ttttccggaa ccggacggcc caagcaacgc gaccatctga ccagaaggaa
    43621 tatccagtga gatatcgttc agcacctggg tgcgaccaaa agacttctta atattggcaa
    43681 tctcaatgct catgatgttc ctcctgctgc gcgcgttttt cctgattttc caggcgccac
    43741 tgcaacatac tctttaaaaa cagggtgata atcgccatca gcgtcaacaa cgcggcggcg
    43801 gtaaaggagc cgacggtgtt gtagtcctgc tccagcaatt caatctgtaa cggcagcgac
    43861 agggtttcgc cacgaatcga gccggaaacc accgataccg cgccaaactc gccaatcgcg
    43921 cgggcgttgg tcagcaccac gccgtaaagc agcgcccagc ggatgttcgg cagcgtgacg
    43981 cgacggaaca tctgccagcc ggacgcgcca agtaaaatcg ccgcttcgtc ttcctggctg
    44041 ccctgactta acattaccgg taccagttcg cgcaccacaa acggacacgt cacgaagatg
    44101 gtgaccagca ccatacccgg ccaggagaac ataatttgca gattatgttc atccagccag
    44161 ccgcccagcg ggccgttaga gccgtagaac agcagataca ccagaccagc aaccaccggc
    44221 gacacggcaa acggaatgtc cagcagcgtc agcaatagct ggcgtccggg gaagttaaag
    44281 cgcgtcacca gccaggccag cagaatgccg aacaccaggt ttaccggtac ggcaatcagc
    44341 gcgatcatca ccgtcagcca gatggcgtgc agcatgtccg gatcggccag attttgtaac
    44401 accggcatca gtcctttgct gaacgcctgt acgaagatat aaatcatcgg caccagcagg
    44461 atgaacgccg ataccagcat cccgatgcca atcagaaacc atttgcccca gttaatcggg
    44521 cgcgcgtcat aacgcttcaa ttgtgtcact tccgccatta atgacctacc acacgccgac
    44581 caaagcgact ttgcagagtg ttaattgaga acagcaacag cagcgatgcc gcgaggatca
    44641 ccgaggcaat cgcgctcgcc gccgggtaat caaactcctg taagcgaaca aagatcatca
    44701 gcgacgtcac ttctgttttc caggcgatat tcccggcgat aaaaatcacc gcgccaaact
    44761 caccgaggct gcgggtaaac gacagcgcca cacccgccac cagcgccgga gatagttccg
    44821 gcagcaccac tttgcgaaaa ctctgccagc gtgttgcgcc gagcgtttct gccgcctctt
    44881 catattccgg gcctaactct tccagcaccg gctgcacggt gcgcaccaca aacggaatgc
    44941 tggtaaaggc catcgccacc gcaatcccca gccaggtata ggtgactttg atatcaaact
    45001 tcgccagcca ttcaccgtaa aaaccgttca cggaaaagag cgaggccagc gttaaaccgg
    45061 cgactgccgt tggcagcgca aagggtaaat ccatcagcgc atcaagcagc gtgcgacccg
    45121 gaaagcgata gcgggttaag atccacgcca tcagcagacc gaaaacgccg ttaaaaatcg
    45181 atgccacaaa cgccgacagc agcgtcactt tataggctgc gaccacctgc gggttggtga
    45241 tcacctccca gtactgcgcc cagctcatct gggccagttg catcaccagc gcggagagcg
    45301 gcagcagcaa aatcaggcac acaaacagca gactggtgcc gaggcttaag gtaaagcccg
    45361 gcagcacgcg tcttgaagaa actgcaaaca ttacttacgc cccgccgcta acagcttgtc
    45421 taactcgccg ccgctggtga agtgggtttt catcacttcc ggccaggagc caaatttatc
    45481 ttccacgcgg aacagctcgg tctgcgggaa tttatctttc agtttgccca tgacttccgg
    45541 gttatttacg cggtagtaat agtcggtgat gatggtttgc gcctgcgggc tgtagagcca
    45601 gttcaggtag gcttttgccg ctttttccgt gccgttggcc tgtacgtttt tatccaccca
    45661 cgccaccggg aattccgcca gaatgttggt tttcggaatc accacttcaa agccctgcgc
    45721 ttcatactgt ttacggatgt tgttcacttc cgactcgaag ctgatcagca catcgcccag
    45781 gccgcgctcg gcaaaagtgg tggtcgcgcc acgaccgcca gtatcgaaca cttcaacgtt
    45841 tttcaggaac tgggtcataa actgttcggt tttggcttta tcaccaccgt cggctttgtc
    45901 cgctgcgccc catgccgcaa ggtaggtata acgcgcgtta cccgacgttt tcgggttcgg
    45961 gaaaatcagc ttcacgtcag agcgtaccag gtcgttccag tcgtggatat tcttcgggtt
    46021 gcccttacgc accaggaagc ccatggtgga gtagaacggc gagctattat tcggcaggcg
    46081 cgactgccag tccgctggga tcagcttgcc tttatcgtgc aggatttgta cgtcggtcac
    46141 ctggttataa gtgacaacgt cggcttttaa accctgcaaa attgccagcg cctgttttga
    46201 tgatccggca tgagattgtt taatcgtcag tttgtcgcca ccgttatctt ttgcccattg
    46261 ttgctcaaac ggcgggttca gggcggcaaa cagctcgcgg gagacgtcat aagaactgtt
    46321 cagcagttcc gttgcctgta catggcccgc cagtagcaga gaagcaacca gcacgagtga
    46381 gttctttttc agtaagttaa cggccattgc gcacccttat aaatttaatg actttctaat
    46441 agccatcata tttataacgg acgtgaaagg agtaacggtt ttatataccg tttggtgatt
    46501 tggaagttga aaagggaata agacgaaatc ctccccacaa atgcagggag gaaacagagg
    46561 tgaatcagat gccgacgcta accgtctccg gcagtgtgct gccgccatca atcacattct
    46621 gtgtaccggt taaatagctg gattcatccg atgcgaggaa ggccgccagt tcgccgactt
    46681 ccagcggatc ggcgaggcga cgcatcggga ttgctttcgc catttcagtc agcaccgatt
    46741 ctggatcttc cgggttcgac tggcgggcaa tgctttccgc cattggcgtg cgaacgtatc
    46801 cagggcagat ggcgttaacg cgaataccag actgcgcgta ctccaccgcc agcgatttag
    46861 tcaggccaac aatcgccgct ttcgttaagg cgtacgccgt ttcgcccggg tctgccacca
    46921 tatcgccagt gactgaagac atcatcacaa tccggccatc tttgcgggca atcatctccg
    46981 gcagcaccgc cttcgtgacg ttccatacgc cttttatatt gatatcaata tggaaatcgc
    47041 gatcttcatc gctcatatca aggaaactgc ccagacgaca aacgcctgcg ttattcacca
    47101 gaatatcaat gcgcccttct ttttccttag cgcgcttaat agctgcggct accgacgccg
    47161 ggtcacgcac atcggcgaca accgccgtac agcgatgacc acggccacac agttcgtccg
    47221 ccagcttttc gatctcaggg gagatatcca gcaagattag gtttgcgcca tgacgcgcaa
    47281 aagttctggc aattccttcg ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc
    47341 ccgtgagttt acccatttta atgctctcct tgttaataca gagcgttaca ctccattccc
    47401 tctgaaaata caggataaac aaaatattgc tgcggatcag ctcactaatt caggaaaatc
    47461 tcaatttagt cgctgcttta tgcagcacct caaacgttac gtcagcgcgc aatcattttc
    47521 agtgacatca catgagtaat attcggactt aatgattcct ttgtgtctat caaatcggga
    47581 tatatgatcc catttatctg caattatttg atcgcaaata ttaaggtcac caccgatgca
    47641 acttgaaaag atgattaccg aaggctcgaa cgccgcctcg gctgaaattg accgcgtatc
    47701 aacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccac tcgccgttga
    47761 gcgcgtactg ccggatatcg ccgcggcgat cgatgttatc cacacccagg taagcggcgg
    47821 cggccgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    47881 cgaatgtccg ccgacctacg gcgtgaaacc gggcctggta gttggtttga ttgctggcgg
    47941 cgaatatgcc attcagcacg cggtggaagg cgcagaagat agccgggaag gtggtgttaa
    48001 tgatctgaaa aatatcggct taaccgcgca ggatgtggtg gttggtattg cggccagcgg
    48061 acgcacgccg tatgtgattg ccgggctgga atacgcgcgc caactcggct gccgtacagt
    48121 gggcatttcc tgtaatccgg ggagtgccgt ttcatccacc gcagagtttg ctattacgcc
    48181 ggtagttggt gccgaagttg ttaccggttc ttcgcgaatg aaagcaggca cggcgcagaa
    48241 actggtgctc aacatgctct ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    48301 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    48361 caaaaacgct accggatgta atgccgaaca agcggaagcg gcgttaattg cttgcgagcg
    48421 caactgtaaa acgaccattg tgatggtact gaaaaatctc gatgccgatg aagctaaaaa
    48481 atgcctggat caacacggcg gttttattcg caaggctctg gaaaaggaat aacccatggc
    48541 taaagagatc agcagtgaac ttctgaacac cattcttacc cgggtcggcg gaccgggaaa
    48601 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcatt
    48661 ggttgatccc gatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgatca
    48721 ggtgcaggtg gtttttgggc caggcaaagc gcatcgtgca gcaaaagcca tgagcgagct
    48781 gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaata aacgccagtt
    48841 aaaagccaga caaacttccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    48901 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    48961 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgatgcgc tgaattttat
    49021 gaaggtgttc agcaaaggcc tgttcacctt cctggtaatt ctggtgggct ataacgcggc
    49081 ccaggcattt ggtggcacgg gcgtaaatgg cgcaattatc gctgcgctct ttttgcttgg
    49141 ttataaccca actgcaacca cgggttacta cgccggattt cacgatttct ttggtctgcc
    49201 catcgatccg cgcggcaata ttatcggcgt gttgatcgcc gcgtgggcct gcgcccgcat
    49261 cgaaggcatg gtgcgccgct ttatgcccga cgacctcgac atgctgttga cctcattaat
    49321 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gcggctggct
    49381 gtttgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgctcg gttgtgcggt
    49441 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    49501 ttatctggcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    49561 aggcgcaggc caggtgggcg cggcgctggc gctctactgg cgggcgcaac cgcacagtgc
    49621 attacgcagt caggtccgcg gggcgattat tcccggtctg cttggcgttg gcgaaccgct
    49681 gatttacggt gtcaccctgc cccgcatgaa accgtttgtt accgcctgtt taggcggcgc
    49741 ggcgggcggt ttgtttatcg gcctgatagc ctggtggggt ctgccgatgg gcttaaacag
    49801 cgcattcggg ccgtctggtc tggtggcgct gccgctgatg acttccgcgc aaggcatcct
    49861 tccggcaatg gcggtttacg ctggcggtat tctggtggca tgggttagtg ggtttatttt
    49921 caccacactc tttggctgcc gtaacgtcaa tctggactga ttatgaaacg gacaatgctc
    49981 tatctttctc tgctggctat tagctgtagc gtcagcgccg caaaataccc tgttctgaca
    50041 gaaagtagtt gatcaagtgt atgcggcgtt aaagcagtaa taaaaaggcc agtcagttgt
    50101 gttactggct ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca
    50161 gcgacggcgc gaagtaatag ccgccagtga ccggtttggt gaaacgcaac atcgcatcac
    50221 gcttaccatc tgtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg
    50281 cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct
    50341 gacgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtc aggtgagagg
    50401 tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca
    50461 tctcttgatc gtgaacgctc atccgattga gctgtttcag gttgtgctcc caacgctgca
    50521 caaacacata gctgccgccc gcatccacgc cgtctttgat aacagccact tcgcggcgcg
    50581 tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcaa
    50641 cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg
    50701 ccgcctgagc gacagagaag ttcacgtcgt gacgcagaga aagaatgtgg atcaacacat
    50761 cgaactgggt cgtcggtgca agacctttac cgtagcccgg aaaatctttc agctcttcgg
    50821 ccccaacgcc gccgctcaga gcgcgccaag tattgttacc aaaggcaacc accgcaccaa
    50881 gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg
    50941 ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc
    51001 aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataataatg
    51061 ccacgcaatc cgtggcattg ttgggcgcta ttttacgtgt tttttcgcgg cggcggtttg
    51121 ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc
    51181 gtccgaaggc attaaccctt cagggccact ccattccccg ctgaactggt agctaatatg
    51241 ttgactgcct tcggctttac attcgacggc acgattatca tcactgtcgg ccttttggca
    51301 attgccaaat gctttgctgt aaaggtcact aaacggtgta ccgattttaa caccggtgtc
    51361 agcaggaata tcgctatcca gcacatcaat gcggctgatc gtgccttgat caccgttaat
    51421 caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc
    51481 ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc
    51541 ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggatga
    51601 tccaaaccag ttccacgggt tagcggccga ccagttaact gacgacatcg tggaacagcc
    51661 ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt
    51721 ggttattcaa taacgttgat tggagtgcaa attcaccaaa aagtgccgtt attctttttc
    51781 ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacgatatc
    51841 cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc gcagccacgg
    51901 ttgccaacaa agtaaaacca cttgcgccaa cagcaacaga aaatagagca catgccagat
    51961 acggggaaat aaagcccgcc gaccactcag caaaaatgcc agcaccgccg gaataccggg
    52021 aatcaacccc agccagaaat tatcgtgatc gggataaaac agattcagca gcgcagtacc
    52081 ctgctcgcgc gacgctccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag
    52141 cacaagccag aagagcaagg gtaaacgcag gcgaccgtgc gcatcataat ggacaggatg
    52201 aaactcagta ctcttcatct tcaatcaaac gcttacccag actcaacacg tcggcgtgtt
    52261 catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct
    52321 gaattttcgg acagccgcga gcaatcagct ttttctccag ccgattaagc aacgcattag
    52381 caatcccacg cccacgaaac tcgggatgca cgccaagata ataagcagac ccgcgatgcc
    52441 cgtcataacc gcccatcacc gttccgacca cttcaccgtt cacctcagcg accagaaaca
    52501 aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca
    52561 gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc
    52621 gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg
    52681 tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca
    52741 actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga
    52801 tacgatataa catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc
    52861 actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg ccctgacgtt
    52921 gtcaggaatg tcgcaagcca tcgccaaaga agaaccgtta aaaaccagta acggacacag
    52981 caagccgaaa gcaaaaaaaa gcggcggcag acgtgtcgtt gttctcgatc ctggccacgg
    53041 cgggattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc
    53101 aattgcaaag aatgtccgtt ccatattgcg taatcatggc attgatgcgc gtttaacgcg
    53161 ttctggcgat acgtttatcc cactgtacga tcgcgttgaa atcgcccata aacatggcgc
    53221 agatctgttt atgtcaattc atgccgacgg ctttaccaat ccgaaagctg ccggtgcttc
    53281 ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcaaaatacc tgtctgaacg
    53341 cgaaaaccgc gccgatgaag ttgccggtaa aaaagcgact gacaaggatc acctattgca
    53401 gcaagtgctg tttgatctgg tgcaaaccga tactattaaa aacagcctga cgctcggctc
    53461 gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc
    53521 cgcgtttgtg gtgttgaaat cgccgtcggt tccgtcggtg ctggtggaaa cctcgtttat
    53581 caccaacccg gaagaagagc ggctgttagg cactgcagcg tttcgccaga aaatcgccac
    53641 agcgattgcc gaaggcgtga tcagttattt ccactggttt gacaaccaga aagcacactc
    53701 gagaaagcgg taagtgatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct
    53761 tcaggatacg atttgtcagc agttgagcgc cgtcgatggc gcagaatttg tcgaagatag
    53821 ctggcagcgc gaagctggcg gcggcgggcg tagtcgggtt ttgcgtaatg gtggtgtttt
    53881 cgaacaggca ggcgtcaact tttcacatgt ccacggtgag gcgatgcctg cttccgccac
    53941 cgctcatcgt ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cgctggtggt
    54001 acatccgcat aacccgtatg tccccaccag ccacgcgaat gtgcgctttt ttattgccga
    54061 aaaaccgggt gcagagcccg tctggtggtt tggcggcggc ttcgatttaa cccctttcta
    54121 tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gcctgccatt
    54181 tggcgaagac gtttatccgc gttacaaaaa gtggtgcgac gaatacttct acctcaaaca
    54241 tcgtaacgaa cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt
    54301 cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttgcc
    54361 aattgtagag cgacgaaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta
    54421 ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct
    54481 gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga
    54541 gtatgattat cagccaaaag atggcagccc agaagcggct ttaagtgagt ttattaaggt
    54601 cagggattgg gtgtaactcc ctcaccccca ctcccgcatc cgctgatgca gcgtcagtga
    54661 cggcttctcg gcaaacagtt gctgataatc cgtggcaaat tgcccaagat gccagaatcc
    54721 ccactgcatg gcggcgtctt ttaccgttgt gctttgtgac cacggactta tgagttcgcg
    54781 gcgcacggcg ttcaggcgaa tacgtttcag ccacgcgttc ggaccaatgc ccaaaatggc
    54841 gtgaaacgcg ttttgtagcg tgcggcggct gacatgcagt tgattacaca agtccagcac
    54901 cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg
    54961 gtaactctga tgactgatgc tttccgccgt caccattggc tgcgcttctt ccagcatcgc
    55021 ccccatcgcc attagcaaat tatcccccag cactttacgc acagcaggct gatgaagatt
    55081 ttccgggttc tcgcaaaatg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt
    55141 atgctgctct ttcacttcca acgccgactg gctacgcagc atatgtaata cccgatccgg
    55201 attatgcaaa aagttagcct gacgggtgat gacatcttca gaaagcacta cgccaaggat
    55261 ggtgtaatcg tccggcgtac ttaattcaaa ctcggttcca ccagggcgcg tcgctatttc
    55321 tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc
    55381 aaaccagaac gaattcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc
    55441 acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg
    55501 atgcagctga tcgtaaatct gctgccaggc ggtaattgtc aaagcatgtt catagacgtc
    55561 cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc
    55621 gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt
    55681 aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata
    55741 acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc
    55801 tttttcgagc ggccagccaa agtgggctgc cacttctcct gccgtcattc cctgacgtac
    55861 tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg
    55921 ttcctttacg ggttgcttag gctggcggtt aaagccagta accagccact gggtgtcatc
    55981 gtctggccga ccgatctcct tgcggctgat gacacgcccg gtacgcatcg cggcagcaca
    56041 acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaggcg
    56101 accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc
    56161 agctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat
    56221 cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt
    56281 aaaggcgtta caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta
    56341 gttggtttca gacggtggcg ggacataggt caccagctgc acgtcggcag atttcaacgc
    56401 tgcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc
    56461 aagcgtgatc ccggcggttg atgagagata agaaccggta cgcgaaacta catgtgccag
    56521 gaaggctgtg ttttctgcgt cattagccca ctggaaagca gcgccatttt caatattcgc
    56581 aaccatcgca tccagaccag cacgcacttc cgccgggttt ggtccaccaa gcataatcag
    56641 cacctcaccg gcagtcggtg acggaccgtg tgcagcgccc gcatacagcg atcgaccata
    56701 taccacttcg accatcgcct gcttggtcgc ttcgtcagcc gcaatgtagg tgacgtcatc
    56761 tgaatcagca gaaatcagcc cgaggctacg aatatgcggc ggcaatttca gttcacgtgc
    56821 aaattcggcg ttaacggagg caatcacccg catggcggtt actgacggtc gaatcaaatc
    56881 taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag
    56941 catacgtttc gccaaatcca caatgacggc tgcggcttca accggcggcg tgccgccctg
    57001 gtgaatgtta gagatacagg tgcgatcggc ctcaacggtg gtcgccatac gcggcgagta
    57061 aacggcgtag caggagaggc tttctgactg ccccaacccc ggacgttcgc ccaccagcag
    57121 gatcactact ttcgcgccta ggatctcacc gatctgatct tcaatcttca cgcgaccata
    57181 acgtacaaag aatggcgtac cgactttcag cccggcttgc ttcaggcccg ccatcagcgg
    57241 cggcaggatc tcttcgtagt tcacggtgat cgcatcagtt gacaggccat cagaaataac
    57301 gacctgcacg tccggattag caacacactg tgctttcagc gcttcaacag cttctgcaca
    57361 caggcggcgg cccatatccg gacgagtcag gtagagattt ttgtcgctga tttcggagcg
    57421 gacttccagc aagccctgcg ctttcaccca ctcttccggt acttctttca gtacggtatc
    57481 tttcgaacga gagtgatcgg ccaggaaacg cagcaacgcc tgggtacgcg gacgcggacc
    57541 ggcacgaccg gtacaaacgc gggccacggt gctgcgacgc agttctgtta atacgtccgc
    57601 gcgatgtggg ttttccacac caatccacgc ttttgcttcc gcagaaccta aatccagcgc
    57661 gcagctttcc gaggtcaccg gtgccgcaca gttggtggtg gcgcactttg cttctgacgg
    57721 cgcgggggcc gcttgtccca ttgacgccat cacgctgcgt acaatttctt caatctgttt
    57781 ttgatccatg atgtgttatc tccgcgtcat cagaagaaca gtgacggatc gcccgcccgt
    57841 ttggtcaggc gaccgtttgc cataatgccc atgctttcca gccagcgttc aaactccggt
    57901 gacggacgca gattcagtaa ctgacgcaca gtggcagtgt cgtggaatgc ggtggtctga
    57961 tagttgagca tgatgtcatc gcccagcggc atccccatga tgtagttaca gcctgcggtg
    58021 gcgagcagga tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg gttggtgtag
    58081 cagcagtcac agcccataga gatgccgctc agcttgccca taaagtgatc ttctaagcct
    58141 gcgcggataa tctggcggtc gttgtagaga tactccggcc caataaaacc gaccacggtg
    58201 ttgacgataa acggatcgta atgacgcgcc agcccatagt tacgcgcttc catcgtcacc
    58261 tggtctgcgc cgaagttagc gccagcggat agcgccgagc cttgtccggt ttcgaagtag
    58321 agacagtttt ccccggcgat acggttgaac tccgcgccca ctgcgcgtgc ttcatcgagc
    58381 atcgccagct ctacaccaaa ctctttcagc cctttttcgc tgccgcagat actctggaaa
    58441 atcagcccgc ctggtgcgcc acgacggatc gcttcgatct gggtggtgac gtgcgccagt
    58501 acgcagccct gagttgggat gttgaatttg tcgatcacgc cataaatggt atccaacacg
    58561 cggcttaagt tttccacgtc gtcagtcacc gggttaacgc caatcaccgc atcgcccacc
    58621 ccaaaggaaa gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc acgggtatcg
    58681 ttcggctgca aacgggcgct aaaggtgccc ggaataccga tggtggtatt ggcctttttg
    58741 atcaccggca ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat cttcgctacc
    58801 gccgccacca cttccgaggt caaccctttg cgagtaaagg caatgtcgtc aacgctggtt
    58861 tcgtcgctca ggacgtattc acgcagttcg ctgatgctcc agtttttaat ctggttgtag
    58921 gccgtttcgt taacgtcgtc ctgaatcagc cgcgtcacgc agtcatcttc ataggcaatc
    58981 accggattat tgcggatgtc tgctacggtc atttccgaca acacctgctt tgccgccacg
    59041 cgctcctgtg agcttgccgc agcaacgccc gccagcacat cccccgaacg cagttcgttg
    59101 gctttagcca gcacctcttt tacatcctta aactgatata cattgccgaa caatgtggtc
    59161 tttagtttca taagtcgttc cctcaggaag gaaatgcgag tgatttcacc gtcaccggca
    59221 caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca
    59281 cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag
    59341 ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga
    59401 cgaaatcgac cagcgcgttg atgaccgtca gtaccgcggc gtaacgcaca ggcagcgagg
    59461 cgggaagcgc cagcacgtac gcgtcagttt tggggcaaag atccagttga atcagcgcct
    59521 gttgccaggc actgaccaga tccgtttcat caatcgggat cgccactggc aaattgcgca
    59581 gcggcagttg tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgagcac
    59641 ccgcgccaat caccgtggcg cgcacggttt gcgccggaaa ctgcacgttc atctcacgca
    59701 gacgcggatg gtcatgcagc gccgttgcca gaagcgggcc gatatcggca aaacagaacg
    59761 ggtcggtggg ctggtggcga taacattcgc ctaccccgcc agaaagcgta atgatttcgg
    59821 gcgtaacacc tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgagagcg
    59881 ttccgtcaat cacttcgaca atcagctctg ccatccggcg ggtaacctgc accagctgcc
    59941 cgccagtcag cgaacgggcg tcagtaccgg caccgaagca ctcatccaca atcatctgcc
    60001 ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccgccga
    60061 cgttgaggca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc
    60121 caccgccgat atcgatattc aggacccgac acagccgttg ttcagaaagg gtttgcgccc
    60181 cggcaccgtg tccggcgatc acggattcaa gatgcggccc ggcgctggcg acgacaaaat
    60241 cgcccagcga ccgggagagc gccatcaccg ccgggcgagc gttgcgggtt tttgcgcttt
    60301 caccggtgat aatgatggca ccagaatcaa cgctttccgg cgcaatgccc gcagcctgat
    60361 attgctcgag gattaaggtt ttcagttccg cttcttttaa accaccctgt ttatcgacag
    60421 gggtaaagaa caccgggctt tgccagctaa tttcgcgttt gatgaattcg tagcgcggca
    60481 cctgcgacac cgccgcacgg ttaaccagct ccaggtggga gaagatcacc tgggtggtgg
    60541 tggtgccgat atcgataccg acgctcagta gctggcgagt gttcacgatt gcgcctccgc
    60601 ttcagtttta gccgcggtcg cgtcttcttt tggcaccagc atcatcgcca cgccaattgc
    60661 cgttacgccg ccgatcaatt tgccgacaat catcgggaag atcatggcgt tcatgttggc
    60721 agcggcgaag cctaaatggt cgcccagggc gaaagcagcg gaaacggcga aggcgcagtt
    60781 gatgactttg ccgcgggtat ccatctgctt catcatgcca aacatcggaa tgttgttggc
    60841 aagcgttgcc accatgccgg ctgccgcgat attgttcata ttcagcacct taccgacgct
    60901 catcagcggt ttttcaaacc agcgagtcag cagcagtacc attggatacg cccctaacag
    60961 aacgcaggag atcgagccga taacttcaat ggcgcgcatc acttcaccgg gtttatcgcc
    61021 aggggccata aagataggat caagacccgg gatcagttcc cagccaagga ggaatttcac
    61081 taccgcagcg gcaagaccga gggtgatcaa tgcaacgagg aatttggcga agatctggaa
    61141 gccgttgatc attttttccg ggatgaattt cagccccagc gccaccagca ccgcaacgat
    61201 aagtaccggg atcatgttca tcaggatcag cgcaaaggtg aattcaacag gctggccgtt
    61261 gatctgcaca ccggagtaca tcgctaccag accaccagcg atacagccaa tcggaatggt
    61321 cactatgccc gccagcacgc cgagcgccac cggaatggaa aacacaatcg ttggccccat
    61381 catcgacccg agaattaacc cagagtatag ccacgctgct acgtcaccgc ccgccagctc
    61441 tttggcgagg aagaagccgc ccatatcgca cgccagcagt gttccggcga acattgacgg
    61501 gtttgcgccg agcatttcgt aaaccgggat aatcaccggt ccgagaacgt gggccagtac
    61561 cggtgccagt gcggtcatac cgaccatcgc caggcccaga gcgcccattg ccataaagcc
    61621 ttcttcgaac tgaccgccgg atccttcgat acttttaccg aacttaccga ggaaacgagc
    61681 agaaccgccg aactgcgaca ggatcctgtc tacggcagct atcagcataa agaacatcat
    61741 gatgtacatg atgatttcgt taattcccat cgcctttact ccctgttagt tgttatttat
    61801 tggcggatgc ggcgtgaacg ccttatccgc cctacatgtg caatcccgaa ggccagataa
    61861 gacgcggcaa gcgtcgcatc cggcaattgc accgcgccac tggcggatgc ggcgtgaacg
    61921 ccttatccgc cctacatgtg caatcccgtc ggccggataa gacgcggaaa gcatcgcatc
    61981 cggcaattgc accgcgccac tggcggatgc ggcgtgaacg ccttatccgc cctacatgtg
    62041 caatcccgtc ggccggataa gacgcggcaa gcgtcgcatc cggcaattgc accgcgccac
    62101 tggcggatgc ggcgtgaacg ccttatccgc cctatatgtg caatcccgtc ggccggataa
    62161 gacgcggcaa gcatcgcatc cggcaattgc accgcgccac tggcggatgc ggcgtgaacg
    62221 ccttatccgc cctacatgtg caatcccgta ggccggataa gacgcggcaa gcgtcgcatc
    62281 cggcatttat tgcgccgctg cgtacaggcc gacaatctgc tccaggctgg cggtacgcgg
    62341 gttactgcgc agacaaatat cttccagcgc ggcctgcgcc catgcgccgt aatgcgcaga
    62401 tgtcgcacca acatcgccca gtcgtttacc aatcccaact tccgctatca gctcacttac
    62461 cgcattaata gcgtcacgat cgtcggattt tttagttcgc agcgcccgac caacctgact
    62521 aaagcgttca cgacagacca tccggttgaa ctccatcacc gttggcagca acatggcgtt
    62581 cgccagaccg tgcggaatat gcagcgccgc ccctggctga tgcgccatcg cgtggcataa
    62641 cccaagcccc gcgctggaaa acgccattcc cgccatacat gaagccagca acatgctctc
    62701 gcgcgcggca aggtcatggc cgtagcccac cgctttcggc agtgatttgc caatcatcgc
    62761 aatggcacca atcgccaggc tgtcggtaaa cggtgtagcg ttcagcgcgc tgtacgcttc
    62821 aatggcatgg gttaacgcat caatgccagt catcgccgtg acatgcgacg gcacgccttc
    62881 ggtcagcaca gcgtcgagaa tcgccacatc cggcatcagc gaggcgtggg ctaacacctg
    62941 cttgcgcccg ctcaccgcgt cgataatcac cgtcacgtta gtcgtttcag agccggttcc
    63001 ggcggtcgtt ggaatggcaa tcaacggcag gcgcggttgc agaacgctgg tttctgacat
    63061 ttctgccagc gtgctatccg ggttcgtcac cagcaacgcc acggctttcg ccgcatccag
    63121 caccgagccg ccgccaaacg cgatcacccc gtcacaacct gattcacgca actgcgccac
    63181 ggctgcacac acgtcggtga tgcacggttc gcccaccgga catggccaga gcgtcatggc
    63241 gataccttta acggccaggc tacgtgtcag cccggcggtc atccctgcct gatgcaaaaa
    63301 gctgtctgcc atcacgaaca gatgtttcaa cccacgcgtt tgcgcttgct gcccgcaact
    63361 gctcaccgcg cccggaccgc aaagcgtcac cggtggaacg ctaaatgttt ttacccgttg
    63421 cagattcagg gtatcgaacg cctgaaaaag cgccgtttgc aattcacttt gcatagatcc
    63481 cctccgcttt ctctcgccca ctactggcga ttgccagcgg ggtcataaac aaactgtgct
    63541 gcggtaaatg cacctgtaac gccggaaatt gtttgcaaaa cagcgccgct acgcccggtt
    63601 gcatacagga accgcccgcc agccataaat ccgtaatgcc ctgcccttca atatgccggg
    63661 ccacaatgtc cgccattttt tcgtacaccg gtttcaccgc gggccaaatc tcgtcaccgt
    63721 gaccgcgttt gtactgctct gcctcttcca gcgaaatacg gcgatttccg gcgagagtca
    63781 gagagatgtg atgtccgccg gtggcttcat ccgccgagta cgtcactttg ccctttttca
    63841 cgatggcaat gccggtagtg ccgccgccga tatccactac accggcgttg tccagttgca
    63901 gcagatccgc aactgcggtt ggctcatcca gcacatggct cacttccagt ccggcagact
    63961 ccagcacgtt gatggaaata cgcgggtccg taccaggagg aaatgaggtc gccgcatggc
    64021 taaaacggcg accgaattgc tgctcaaggg tgtcgagatg gcgacgaaca atggtgaccg
    64081 caccgaagaa atcccagacg atgccgtcgc ggaccacatc ggcccagtcg aggcataccg
    64141 ccaccggctg accgttgcgg tcgacaacca tcgacaccac atcgcaagtg cccagatcca
    64201 cccccagcca cagcggagat tccgtcgcgg caggcgtctg gttacacagc gttgccgccg
    64261 tttgcagacg tggggtgagc cattgttcat cgtgcgccat cggttactcc ttaaacaatg
    64321 cgaaacgcat cgactaatac acagcgacgc agacggacaa acgtccgcgc gctggttacc
    64381 ccttcaccgg ttggcgtggt gatggtcatc gtggtccagc cttccccgcc cagccccagc
    64441 ccggcaatac acggtccgtt cttaacgaaa atgctggtat caatggcgtt cgccatctgg
    64501 ttcatgtttt cgatattgcg cgagtgcatt gccgccgtgt ggtggcaacc gccttccagt
    64561 ttcaccgcca gcgcaatggc atccgccacg ttggcgacgc gcacgacggg cagcaccggc
    64621 atcatcagtt cggtcacggc aaacggatgt tctgcggtgg tttctacaaa cagcagtcgc
    64681 gtttgttccg gaactttaag gtcgattgcc gccgcgattt tcgccgcatc gcgaccaacc
    64741 cagtcacggc tgacggtgcc ttttccgcac tcgtcgatat ttttcagcaa caccgattgc
    64801 agttgctgcg cctgctctgc ggtcagtttc accgcatgct ggccttccat cagacgcatc
    64861 agttcatcgg ctacgctatc cacaacaatc agtacctttt cgtcggcaca aatgatgttg
    64921 ttatcgaaag acgcgccttt gacgatggac tgagcagcac gggcgaggtc ggcggtttca
    64981 tccaccacta ccggcgggtt gccagcgcct gcggcaatca gacgtttatt ggtgtgttta
    65041 cgcgccgctt ccactaccgc ttcgccgccg gtcaccacca gcaggccgat acccggaaac
    65101 ttgaacaagc gttgcgcggt ttcgatatcc ggatttgcta cggtaaccag taagttttcc
    65161 ggcccgcctg cggcaacaat cgcctggttg agcagcgtaa tcgcccgctg ggagactttt
    65221 ttcgccgccg gatgcggggc aaaaatgacg ctgttgcccg cggcaatcag gctgatggcg
    65281 ttgttaatta cggttgccgc cgggttagtg gaaggcgtca ccgaagccac cacgccccag
    65341 ggtgcgtttt caattagggt caggccgttg tcgccagtca gcacttgcgg agagaggcac
    65401 tcaacgcctg gtgtgccgcg cgcctgagcg acgtttttgg caaatttatc ttcaacgcgc
    65461 cccatgccgg tttcactgac ggcaagttcc gctaaatctc tggcgtgttt ttcgcctgct
    65521 tcacgaatgg cagcaatggc taactggcgc attgccacgc tttttaaccc ttgctgggcg
    65581 actttggctg ccgcaacggc gtcatccagg gacgcgaaaa cgcccatctc atgaacggcg
    65641 gcgggcggcg tgtcactgct ttgcattttc agcagtaccg ctttcaccac ctgttcaata
    65701 tcctgttgat tcatgatgtt ctgccttatt tgtggaaaat gacctgaccg ccagacacca
    65761 cctcatcgac aatgccaatc acgcacagat cgaccggtga cgtttcgctt ttatgcgcct
    65821 ggcgggcgga actgccactc accagtaaca cccactcccc ggttcccgcg ccaatattgt
    65881 cgatggcgac ggcgcattgc ccgtcaggat taccttgtgg atcaatcatt tccaccatca
    65941 gcaatttgtc atgcgccagt ccgtgatggc gtacggtaca aacaatttgt ccagtgacga
    66001 ctgccagttt catacccgcc tccgtggcgt atttcaggta aaagctcccc ctaccctccg
    66061 cagaaggtaa aatgaaaaag gagagagcgt gacgcccgaa tcgacgtcac acaaggtgat
    66121 tacaggttgc tgctatcgcc tttcaggccg atcgggaaga cttcttccag gtcgccgtgc
    66181 ggacgcggga taacgtgtac agataccagc tcgccgatac gctgtgccgc tgcggcacct
    66241 gcatcggtcg cggctttaca ggctgcaaca tcaccacgaa ccatggcagt acacaggccg
    66301 ccgccaatct gcttaacacc aaccagcttg acgcgcgcag ctttaaccat cgcgtcagaa
    66361 gcctcaatca gtgcaaccag gccccgggtt tcgatcattc ctaatgcttc cattgtgctt
    66421 tcctctttat cagggtccag aacgggaccg ttcattcaac cagtgtttgt aaactgcttt
    66481 cgtggttcac ttctgtctga cgcggcacag ctgccaccag cgccagttcg ataatttcct
    66541 gcacgctaca accacgagag agatcgtgca tcggcgcggc aagtccttgt atcaatggcc
    66601 cgacggcacg atatccgcca agtcgttgtg cgattttgta gccaatattt cctgcctcca
    66661 gcgacggaaa aaccatcaca ttagctttgc cctgtaacgg gctggcaggc gctttttgcg
    66721 ccgccacttc cggcacgaag gcggcgtcaa actgcaactc gccatcgaca accagctttg
    66781 gtgcgcgctc acggacgatt tctgtcgcct gcttgacgtt ggcaacgcag gggtgacggg
    66841 cactaccgtt gctggaaaac gacagcatcg ccacgcgcgg ttcttctccg gtgatggcgc
    66901 gccaggtttc ggcgctggca agcgcgatat ccgccagttg tgccgccgtc ggctgtggca
    66961 ccacgctgca atcggcaaag cccaacgccg gaccgctgta ctgtggcagc atcaggaaaa
    67021 tggatgagag cgttttacag cctggctgca agccgataat gcgtaatccg gcacgcagca
    67081 cattcgccgt ggaagagagg ttgcccgcga tacagacatc cgctttaccg gcgctgacca
    67141 ttgcggcggc gaacatcagc ggatcggtaa gtttttccag cgcatccggc ggcgtttttt
    67201 cgcccgcgcg ggccagccag cgatgagcaa attcttcccg cattgcgagg ttgccatgcg
    67261 gatctatcac ctgtagcccg tccatcgcca cgccgtgact gagcgcaaac tgacgaagtt
    67321 caaacggatt ggcgaccaga atgggcgttg ccagaccttg ttgatgtaaa tattgcgcag
    67381 ctttcagcac acgttgatct aacgcatccg gaaaaactac tctggcgggc gctcgcaacg
    67441 ccagttcacg acaacgttta ataatcattg cgtttccccc agtcgttgtt ggatctgctt
    67501 caccgttagc ggctgcttcg ttacgctcag gatcatcatc acgtagaccg tgctggaaag
    67561 gcggttcagc gcctgcaaaa tgtccgggcg taatacttca aagctgcggg tgataaagac
    67621 ctgcgccgct acggtttcgg tttcccgcac tttggtgcgt aacagattca gcaacgccgc
    67681 atcgcgcccg tggctggctt ccggcaccag atgatcgtgg tcgagataac gcagcggctg
    67741 gtgagaaagc cgatgcagat cttcgtcaga aagcccgaca atcgcctgac agcccaacgg
    67801 ttcccccagc gcatcggcac gcataatgtt gccaagacgc gaacggatat ccgccagcca
    67861 cggctgccac ggttccgcca gttcaatttg cagccacact gccagcgcaa tggtgctgtc
    67921 gagaacagcg cgaaaaccca gacgcggatc gcttttggcg accatttttt ccgccgacag
    67981 gtgagtcagc gtatccggct tttttgctac cggctggcgg cacagttcgc agcacgcctg
    68041 cggatgttca tcgctactgg tcagcccgtg aacgggctgc ggctgctgct gttcatcgtc
    68101 aacaaacagg cggccctgct cgtcaataaa cttgatgcgc agatggcggc tttccagtaa
    68161 ctcccgggca gagggcgtca ggcgactgtc cgcagggaga tggatctctg cgccttcgct
    68221 gagcgtatgg ttcgctctta gccatgcttc ggtgatgaaa tctttcatag ggattgccag
    68281 ttagccggcc aggcgacata caggaatttc acgctggatg tcgtaccaaa ttcgatactg
    68341 gagcctttcg ggataaacat cacatcgccc gctttggcga tcatagtttc gccttcgtgg
    68401 cgaacatgca gctccccttc cagcaccatg tcgatttcgt cgtagttcag ggtccacggg
    68461 aagaatgcgt tctcccactg cataaaacca gcggccatac tgctgccgtc gtctccggtc
    68521 acgagatcag tcaagcctac gcagtgtggc tgtgcgccgt cgaaacgacc aaacttgacg
    68581 ctgctgccat caataacttt gatgccgcct ttgccggtca ccgatttaaa gctcggctgc
    68641 agtgcaccct gttccagcga ctgtttttcc ttcatcactt tttccatcag ctgcgcgacc
    68701 aggctttcgg taaactgacc ttccggcagt tgggcgataa tggtttcacg gatgcgctgg
    68761 ctttcggttt tatcggctga tgcgctggcg ggaacagacg ccgttaccgg gatcgattca
    68821 tcacattcgg tgatggtaaa acccaacaga tcagccactt cacgagcttc cggggtaata
    68881 atgctggcgc gcagaaccac ggacattgcc agttcgccgc gcgcgtgtgc ttcacgaata
    68941 tcattcgctg tgatgagttt tttcacctgc ttcctcctgt ttggtgagag atgccagata
    69001 atccaccagt tgctgcacgc tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc
    69061 gaagcccgtc tcaagcaata actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt
    69121 tttactgatg acggcgattt gccgtttgct gacgccaata tccagcaacc cggcaggcaa
    69181 ccgactttcc gggtcattcg cgccgtggac atagatcagc atgtccacat cctgtagcgt
    69241 ggtaattaag gcgtgatacc agcggggatg gctaaaatat tcacccggtg tatcaatatc
    69301 gccattatca ttaaattcca cggtctgtgt ttttcttgcg agggtataat tcccctgcaa
    69361 cgcattaaat aacgttgttt ttcctgcccc gacggtaccc acaaacgcaa ttcgtttcat
    69421 gaatgcctcg tattaacttt tggtcatttc acaaagcgta taatttaata agcgtcctaa
    69481 accactgacg gtttgtgata acgcttcttc tactgcgccc accgagccat aaatcaccag
    69541 cgcgccgcta aaacggtcga gaaaaccgat atgcacatcg gcggctttca acgctaaatc
    69601 accggcgatc atcgccgttt ctccgggagt tagcgtcata atgccgattg cgcccgcatc
    69661 cggaacgccg attttcttcg ccagttcctc gccgggatgc gcgatgagat gcgccagtgt
    69721 gacctgttta ccgggcacaa attcctgaat gatgcgttct ttatccatga gtcgccgcct
    69781 gtgaaaaact ttcgtgtatc gtgacaattt ttcgtcagag taaataacaa aattcggcaa
    69841 gcgcgtttaa aaggtgaggt agatcactaa gaatggagag atatttgctg tggtgcataa
    69901 actt
//
LOCUS       DAZFRE010000014        66350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFRE010000014 DAZFRE010000000
VERSION     DAZFRE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.014"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            53..1468
                     /gene="tnaA"
                     /locus_tag="ACHKUI_001108"
     CDS             53..1468
                     /gene="tnaA"
                     /locus_tag="ACHKUI_001108"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6597351.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYRYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1560..2807
                     /gene="tnaB"
                     /locus_tag="ACHKUI_001109"
     CDS             1560..2807
                     /gene="tnaB"
                     /locus_tag="ACHKUI_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6597352.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKKKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2939..4114
                     /gene="mdtL"
                     /locus_tag="ACHKUI_001110"
     CDS             2939..4114
                     /gene="mdtL"
                     /locus_tag="ACHKUI_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO6597353.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLNFPWQSLF
                     WTMAIMGVAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCCSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4089..5048
                     /gene="yidZ"
                     /locus_tag="ACHKUI_001111"
     CDS             4089..5048
                     /gene="yidZ"
                     /locus_tag="ACHKUI_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6597354.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5205..5954
                     /gene="yieE"
                     /locus_tag="ACHKUI_001112"
     CDS             5205..5954
                     /gene="yieE"
                     /locus_tag="ACHKUI_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6597355.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSVSYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5976..6542
                     /gene="yieF"
                     /locus_tag="ACHKUI_001113"
                     /gene_synonym="chrR"
     CDS             5976..6542
                     /gene="yieF"
                     /locus_tag="ACHKUI_001113"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6597356.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6596..7933)
                     /gene="adeP"
                     /locus_tag="ACHKUI_001114"
     CDS             complement(6596..7933)
                     /gene="adeP"
                     /locus_tag="ACHKUI_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6597357.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8100..8765
                     /gene="yieH"
                     /locus_tag="ACHKUI_001115"
     CDS             8100..8765
                     /gene="yieH"
                     /locus_tag="ACHKUI_001115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6597358.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8832..9305
                     /gene="cbrB"
                     /locus_tag="ACHKUI_001116"
     CDS             8832..9305
                     /gene="cbrB"
                     /locus_tag="ACHKUI_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO6597359.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(9387..10109)
                     /gene="yieK"
                     /locus_tag="ACHKUI_001117"
     CDS             complement(9387..10109)
                     /gene="yieK"
                     /locus_tag="ACHKUI_001117"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6597360.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10124..11293)
                     /gene="yieL"
                     /locus_tag="ACHKUI_001118"
     CDS             complement(10124..11293)
                     /gene="yieL"
                     /locus_tag="ACHKUI_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO6597361.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11320..12936)
                     /gene="bglH"
                     /locus_tag="ACHKUI_001119"
     CDS             complement(11320..12936)
                     /gene="bglH"
                     /locus_tag="ACHKUI_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO6597362.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13022..14416)
                     /gene="bglB"
                     /locus_tag="ACHKUI_001120"
     CDS             complement(13022..14416)
                     /gene="bglB"
                     /locus_tag="ACHKUI_001120"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO6597363.1"
                     /translation="MKAFPETFLWGGATAANQMEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTAFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTITRKKSFEW
                     YAEVIKTRGLSLKK"
     gene            complement(14435..16309)
                     /gene="bglF"
                     /locus_tag="ACHKUI_001121"
     CDS             complement(14435..16309)
                     /gene="bglF"
                     /locus_tag="ACHKUI_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO6597364.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKDNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWATEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16443..17279)
                     /gene="bglG"
                     /locus_tag="ACHKUI_001122"
     CDS             complement(16443..17279)
                     /gene="bglG"
                     /locus_tag="ACHKUI_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO6597365.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(17565..18290)
                     /gene="phoU"
                     /locus_tag="ACHKUI_001123"
     CDS             complement(17565..18290)
                     /gene="phoU"
                     /locus_tag="ACHKUI_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6597366.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(18305..19078)
                     /gene="pstB"
                     /locus_tag="ACHKUI_001124"
     CDS             complement(18305..19078)
                     /gene="pstB"
                     /locus_tag="ACHKUI_001124"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6597367.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(19169..20059)
                     /gene="pstA"
                     /locus_tag="ACHKUI_001125"
     CDS             complement(19169..20059)
                     /gene="pstA"
                     /locus_tag="ACHKUI_001125"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6597368.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKRKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20059..21018)
                     /gene="pstC"
                     /locus_tag="ACHKUI_001126"
     CDS             complement(20059..21018)
                     /gene="pstC"
                     /locus_tag="ACHKUI_001126"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6597369.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21105..22145)
                     /gene="pstS"
                     /locus_tag="ACHKUI_001127"
     CDS             complement(21105..22145)
                     /gene="pstS"
                     /locus_tag="ACHKUI_001127"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6597370.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            22948..23187
                     /locus_tag="ACHKUI_001128"
     CDS             22948..23187
                     /locus_tag="ACHKUI_001128"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFO6597371.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(23375..24352)
                     /gene="intI2"
                     /locus_tag="ACHKUI_001129"
                     /pseudo
     CDS             complement(23375..24352)
                     /gene="intI2"
                     /locus_tag="ACHKUI_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            24684..25157
                     /gene="dfrA1"
                     /locus_tag="ACHKUI_001130"
     CDS             24684..25157
                     /gene="dfrA1"
                     /locus_tag="ACHKUI_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO6597372.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            25252..25776
                     /gene="sat2"
                     /locus_tag="ACHKUI_001131"
     CDS             25252..25776
                     /gene="sat2"
                     /locus_tag="ACHKUI_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFO6597373.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            25834..26669
                     /gene="aadA1"
                     /locus_tag="ACHKUI_001132"
                     /pseudo
     CDS             25834..26669
                     /gene="aadA1"
                     /locus_tag="ACHKUI_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            26698..27195
                     /locus_tag="ACHKUI_001133"
     CDS             26698..27195
                     /locus_tag="ACHKUI_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597374.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            27256..27627
                     /locus_tag="ACHKUI_001134"
     CDS             27256..27627
                     /locus_tag="ACHKUI_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597375.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            28037..28414
                     /locus_tag="ACHKUI_001135"
     CDS             28037..28414
                     /locus_tag="ACHKUI_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597376.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            28464..28589
                     /locus_tag="ACHKUI_001136"
     CDS             28464..28589
                     /locus_tag="ACHKUI_001136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597377.1"
                     /translation="MKVVYLAIQAATKKWTMPIRNWKPAMNRFMIEFGDRLNGHL"
     gene            complement(28645..30261)
                     /locus_tag="ACHKUI_001137"
     CDS             complement(28645..30261)
                     /locus_tag="ACHKUI_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFO6597378.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(30262..31788)
                     /locus_tag="ACHKUI_001138"
     CDS             complement(30262..31788)
                     /locus_tag="ACHKUI_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFO6597379.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(31791..33458)
                     /locus_tag="ACHKUI_001139"
     CDS             complement(31791..33458)
                     /locus_tag="ACHKUI_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6597380.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(33455..35563)
                     /locus_tag="ACHKUI_001140"
     CDS             complement(33455..35563)
                     /locus_tag="ACHKUI_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6597381.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(35550..36371)
                     /locus_tag="ACHKUI_001141"
     CDS             complement(35550..36371)
                     /locus_tag="ACHKUI_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFO6597382.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(36531..38360)
                     /gene="glmS"
                     /locus_tag="ACHKUI_001142"
     CDS             complement(36531..38360)
                     /gene="glmS"
                     /locus_tag="ACHKUI_001142"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6597383.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(38521..39891)
                     /gene="glmU"
                     /locus_tag="ACHKUI_001143"
     CDS             complement(38521..39891)
                     /gene="glmU"
                     /locus_tag="ACHKUI_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6597384.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(40244..40663)
                     /gene="atpC"
                     /locus_tag="ACHKUI_001144"
     CDS             complement(40244..40663)
                     /gene="atpC"
                     /locus_tag="ACHKUI_001144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6597385.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(40684..42066)
                     /gene="atpD"
                     /locus_tag="ACHKUI_001145"
     CDS             complement(40684..42066)
                     /gene="atpD"
                     /locus_tag="ACHKUI_001145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6597386.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(42093..42956)
                     /gene="atpG"
                     /locus_tag="ACHKUI_001146"
     CDS             complement(42093..42956)
                     /gene="atpG"
                     /locus_tag="ACHKUI_001146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6597387.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(43007..44548)
                     /gene="atpA"
                     /locus_tag="ACHKUI_001147"
     CDS             complement(43007..44548)
                     /gene="atpA"
                     /locus_tag="ACHKUI_001147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6597388.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(44561..45094)
                     /gene="atpH"
                     /locus_tag="ACHKUI_001148"
     CDS             complement(44561..45094)
                     /gene="atpH"
                     /locus_tag="ACHKUI_001148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6597389.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(45109..45579)
                     /gene="atpF"
                     /locus_tag="ACHKUI_001149"
     CDS             complement(45109..45579)
                     /gene="atpF"
                     /locus_tag="ACHKUI_001149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6597390.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(45641..45880)
                     /gene="atpE"
                     /locus_tag="ACHKUI_001150"
     CDS             complement(45641..45880)
                     /gene="atpE"
                     /locus_tag="ACHKUI_001150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6597391.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(45927..46742)
                     /gene="atpB"
                     /locus_tag="ACHKUI_001151"
     CDS             complement(45927..46742)
                     /gene="atpB"
                     /locus_tag="ACHKUI_001151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6597392.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(46751..47131)
                     /gene="atpI"
                     /locus_tag="ACHKUI_001152"
     CDS             complement(46751..47131)
                     /gene="atpI"
                     /locus_tag="ACHKUI_001152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6597393.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(47748..48371)
                     /gene="rsmG"
                     /locus_tag="ACHKUI_001153"
     CDS             complement(47748..48371)
                     /gene="rsmG"
                     /locus_tag="ACHKUI_001153"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6597394.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(48435..50324)
                     /gene="mnmG"
                     /locus_tag="ACHKUI_001154"
     CDS             complement(48435..50324)
                     /gene="mnmG"
                     /locus_tag="ACHKUI_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6597395.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(50703..51146)
                     /gene="mioC"
                     /locus_tag="ACHKUI_001155"
     CDS             complement(50703..51146)
                     /gene="mioC"
                     /locus_tag="ACHKUI_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6597396.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(51236..51694)
                     /gene="asnC"
                     /locus_tag="ACHKUI_001156"
     CDS             complement(51236..51694)
                     /gene="asnC"
                     /locus_tag="ACHKUI_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6597397.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            51846..52838
                     /gene="asnA"
                     /locus_tag="ACHKUI_001157"
     CDS             51846..52838
                     /gene="asnA"
                     /locus_tag="ACHKUI_001157"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6597398.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWVGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(52843..54294)
                     /gene="viaA"
                     /locus_tag="ACHKUI_001158"
     CDS             complement(52843..54294)
                     /gene="viaA"
                     /locus_tag="ACHKUI_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6597399.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(54288..55784)
                     /gene="ravA"
                     /locus_tag="ACHKUI_001159"
     CDS             complement(54288..55784)
                     /gene="ravA"
                     /locus_tag="ACHKUI_001159"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6597400.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            56007..57875
                     /gene="kup"
                     /locus_tag="ACHKUI_001160"
     CDS             56007..57875
                     /gene="kup"
                     /locus_tag="ACHKUI_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6597401.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIVNPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            58042..58461
                     /gene="rbsD"
                     /locus_tag="ACHKUI_001161"
     CDS             58042..58461
                     /gene="rbsD"
                     /locus_tag="ACHKUI_001161"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6597402.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            58469..59974
                     /gene="rbsA"
                     /locus_tag="ACHKUI_001162"
     CDS             58469..59974
                     /gene="rbsA"
                     /locus_tag="ACHKUI_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6597403.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            59979..60944
                     /gene="rbsC"
                     /locus_tag="ACHKUI_001163"
     CDS             59979..60944
                     /gene="rbsC"
                     /locus_tag="ACHKUI_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6597404.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            60969..61859
                     /gene="rbsB"
                     /locus_tag="ACHKUI_001164"
     CDS             60969..61859
                     /gene="rbsB"
                     /locus_tag="ACHKUI_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6597405.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            61985..62914
                     /gene="rbsK"
                     /locus_tag="ACHKUI_001165"
     CDS             61985..62914
                     /gene="rbsK"
                     /locus_tag="ACHKUI_001165"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6597406.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            62918..63910
                     /gene="rbsR"
                     /locus_tag="ACHKUI_001166"
     CDS             62918..63910
                     /gene="rbsR"
                     /locus_tag="ACHKUI_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6597407.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(63876..65303)
                     /gene="mdtD"
                     /locus_tag="ACHKUI_001167"
     CDS             complement(63876..65303)
                     /gene="mdtD"
                     /locus_tag="ACHKUI_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6597408.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(65326..66018)
                     /gene="yieP"
                     /locus_tag="ACHKUI_001168"
     CDS             complement(65326..66018)
                     /gene="yieP"
                     /locus_tag="ACHKUI_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6597409.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        16743 a        16696 c        15908 g        17003 t
ORIGIN      
        1 aatgtaatat tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa
       61 ctttaaacat ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtaccac
      121 tcgcgcttat cgtgaagagg caattattaa atccggtatg aacccgttcc tgctggatag
      181 cgaagatgtg tttatcgatt tactgaccga cagcggcacc ggggcggtga cgcagagtat
      241 gcaggccgcg atgatgcgcg gcgacgaagc ttacagcggc agccgcagct actacgcgtt
      301 agccgagtca gtgaaaaata tctttggtta tcaatacact attccgactc accagggccg
      361 tggtgcagag caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct
      421 ggatcgcagc aaaatggtgg cgttctctaa ctatttcttt gataccacgc agggccatag
      481 ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt
      541 gcgttacgac tttaaaggca actttgacct tgagggatta gaacgcggta ttgaagaagt
      601 tggcccgaat aacgtgccgt atatcgttgc aaccatcacc agtaactccg caggtggtca
      661 gccggtttca ctggcaaact taaaagcgat gtatagcatc gcgaagaaat acgatattcc
      721 ggtggtcatg gactccgcac gctttgccga aaacgcctat ttcatcaagc agcgtgaagc
      781 agaatacaaa gactggacca tcgagcagat cacccgcgaa acctacagat atgccgatat
      841 gctggcgatg tccgccaaga aagatgcgat ggtgccgatg ggcggcctgc tgtgcatgaa
      901 agacgacagc ttctttgatg tgtacaccga atgcagaacc ctttgcgtgg tgcaggaagg
      961 cttcccgaca tatggcggcc tggaaggcgg cgctatggag cgtctggcgg taggtctgta
     1021 tgacggcatg aatctggact ggctggctta tcgtatcgcg caagtgcagt atctggtcga
     1081 tggtctggaa gagattggcg ttgtctgtca gcaggcgggc ggtcacgcgg cattcgttga
     1141 tgcaggtaaa ctgttgccgc atatcccggc agatcagttc ccggcacagg cgctggcctg
     1201 cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg
     1261 cgatccgaaa accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc
     1321 gcgcgcaaca tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtgaa
     1381 agagaacgcg gcgaatatta aagggttaac ctttacctac gaaccgaaag tattgcgtca
     1441 cttcaccgca aaactgaaag aagtttaatt aaaacactca gagtggctat aaggatgtta
     1501 gccactctat taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca
     1561 tgactgatca atcagaaaaa aaacactctg cattttgggg tgttatggtt atagccggta
     1621 cagtaattgg cggtgggatg tttgctttac ctgttgacct tgccggagcc tggttttttt
     1681 ggggcgcttt tattcttatt attgcctggt tttcaatgtt acattcaggg ctattattat
     1741 tagaagcaaa cttaaattat cccgtcggat ccagttttaa caccatcacc aaagatttaa
     1801 tcggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca
     1861 cttatgccta tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg
     1921 gctatcacgc taatccacgt attgtcggga tctgcactgc cattttcgtt gccagcgtat
     1981 tgtggataag ttcgttagcc gccagtcgta ttacctcatt gttccttggg ctgaagatta
     2041 tctcctttgt aattgttttt ggttctttct tcttccaggt cgattactcc attctgcgcg
     2101 attccaccag caccactgcg gggacgtctt acttcccgta tatctttatg gctttgccgg
     2161 tgtgtctggc gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca
     2221 aaaagaaaga caaactaatc aaaagcgtgg ttttcggttc gctgctggcg ctggtgattt
     2281 atctcttctg gctctattgc acgatgggga atatcccgcg agaaagcttt aaggcgataa
     2341 tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg
     2401 ggattatcga gttttgcctg ctggtgttct ccaacttagc tgttgccagt tcgttctttg
     2461 gtgtcacgct ggggttgttc gattatctgg cggatctgtt taagattgat aactcccacg
     2521 gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc tgctttgttg tatctgatct
     2581 tcccgaacgg cttcatttac gggatcggcg gtgccggact gtgtgccacc atctgggcgg
     2641 taataattcc tgctgtactg gcaatcaaag cccgcaagaa gtttcccaat cagatgttca
     2701 cggtctgggg cggcaatctt attccggcga ttgtcattct ctttggcata accgtaattt
     2761 tatgctggtt cggtaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaggaag
     2821 ccagccattc gctggcttct tgcctgtcag gaaatcactt atgtccaaat ggcaactcgc
     2881 ctgattctcc ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat
     2941 gtcccgcttt ttgatttgta gttttgccct ggttttactt tatcccgcag ggattgatat
     3001 gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca
     3061 tattgcgttc tccgtttatc tggcggggat ggcggctgcg atgttatttg ccggtaaagt
     3121 ggccgatcgt tcagggagaa agcctgtcgc catacccggc gcggcgctat ttattatcgc
     3181 ctcggtgttc tgttcactgg ctgaaaccag cgcgttattt cttgcagggc gatttctaca
     3241 gggactgggc gcgggctgtt gttacgtagt ggcgtttgcc attttgcgcg acacgctgga
     3301 tgatcgacgt cgggctaaag tgctgtcgtt actcaacggc attacctgca tcattccggt
     3361 gttagcgcca gtgctcggac atctgattat gcttaacttc ccatggcaga gtctgttctg
     3421 gacgatggca ataatgggtg tcgcggtact gatgttgtct ttgtttattt taaaagaaac
     3481 gcgccctgcg gcccccgcag cttcggacaa accacgagaa aatagcgagt cactgcttaa
     3541 ccggtttttc ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacctt
     3601 cgtcaacacg tcgccagtat tgctgatgga aatcatgggt tttgagcgcg gagaatacgc
     3661 caccattatg gcgttgaccg ctggcgtcag catgaccgtt tcattctcca cgccgtttgc
     3721 gctgggaatt tttaagccac gtacgttgat gatcacctca caggtgttat tcctggcggc
     3781 ggggatcacc cttgccgttt caccttccca tgcggtttcg ctgtttggta tcacgctgat
     3841 ttgcgccggt ttctcggtag gttttggtgt ggcgatgagt caggcgttag ggccgttttc
     3901 attacgcgcg ggcgtagcca gctcgacctt aggtattgcg caagtttgct gttcgtcact
     3961 gtggatttgg ctggcggcgg tggttggtat cggcgcatgg aatatgctga tcgggattct
     4021 gattgcctgt agcatagtaa gcctgttgct gattatgttc gtcgcgcctg gacgcccggt
     4081 tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac
     4141 tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcgg
     4201 cggtgagtaa gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact
     4261 caccgctggg gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat ctggcggagt
     4321 ggatgcaaat gagcaacctg ctgctggata aaccgcacca ccagacaccg cgcggcctga
     4381 agtttgagct ggcagcagaa tcaccgctaa tgatgatcat gcttaatgcg ctgtcgaaac
     4441 ggatctacca acgttacccg caggcgacca tcaaattacg taactgggat tacgattcct
     4501 tagatgccat tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agtcatcccc
     4561 gttcgcggga gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg
     4621 atgtgccctg cgtctggtta cgcaaagatc atccggcact gcatgaagca tggaatctgg
     4681 acaccttttt acgttatccg catatcagca tttgctggga acagagcgat acctgggcgc
     4741 tggacaatgt gttacaggag ctggggcgcg aacgcacgat tgctatgagc ctgccggaat
     4801 tcgagcagtc actgtttatg gcagcacagc ccgacaatct gctactggcg accgcgccgc
     4861 gctactgcca gtactacaat caactccacc aactgccgtt ggttgctctt cctctcccgt
     4921 ttgatgaaag ccagcaaaaa aagctggaag tcccttttac ccttctgtgg cataaacgga
     4981 acagccgtaa tccgaagatc gtctggttac gggaaaccat taaaaatctt tacgcgtcga
     5041 tggcataacc gaatcgaatg aaacgcgacc caatttcaca atgaaatgta aaaaagttgt
     5101 aataaactta tctgaatcga acttttagcc gctttagtct gtccatcatt ccagtaaatg
     5161 attactcttg tattcataat ggaccattaa gcatggagcg aaaaatggcg actcactttg
     5221 cccgagggat tttaacggaa ggacatctga tttctgttcg tctcccctcc cagtgtcatc
     5281 aagaagcccg aaacattccc cctcatcgtc aaagccgttt tctggcgtcc agaggtttac
     5341 tcgcagaact gatgttcatg ctgtatggca ttggcgaatt gccggaaatc gtcaccctgc
     5401 cgaaaggaaa accggttttc agcgataaaa atttgccttc gttttctgtt tcctatgccg
     5461 ggaatatggt cggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat atggaactac
     5521 agcgtgcgac acgcgggttt catagcccac acgcgcccga taaccacacc ttttccagca
     5581 atgaatcgct atggatcagt aaacaaaacg atcctaacga agcgcgggcg cagctcatca
     5641 cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat ccgcgcgatc
     5701 tgcaactact gcccattgcc ggacgcctga aatgtgctca tgtaaatcat gtagaagcgt
     5761 tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt cacgcccacg attgaaaagc
     5821 tcagtgtctg ggagttagat ggcaaacatg gctggaaaag cctgcccgat attcacagcc
     5881 gcgccaacaa tcctaccagc cggatgatgc gttttgccca gctctctacc gtgaaggctt
     5941 tttcgccaaa ttgatagaca accacaggag tcatcatgtc tgaaaaattg caggtggtta
     6001 cgttactggg gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt accctgccga
     6061 aaattgctcc ggcgagcatg gaaatcaatg cgttaccttc cattgccgac attcccctgt
     6121 atgacgctga cgtacagcag gaagacggtt ttccagcaac agtagaagct ctggcggaac
     6181 agatccgtca ggctgacggt gtggtaatcg tcacgccgga atataactac tcggtaccgg
     6241 gtgggctgaa aaatgccatc gactggcttt cccgcctgcc ggatcaaccg ctggcaggta
     6301 aaccggtatt gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc tgtcagtatc
     6361 acctgcgcca gattctggtt ttcctcgatg caatggtgat gaacaagcca gaatttatgg
     6421 gcggcgtgat tcagaacaaa gttgatccgc aaaccgggga agtgattgat cagagtacgc
     6481 tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga gttaagatct
     6541 aaataaaaaa cccgccagca atcatgcatg gcgggttttt aacgcgctat cgattttagt
     6601 gagcgtcgat aaagacaatc ttcaggataa acagcagcgc aacgatgatt acgcacgggc
     6661 taaggtcacg cagacgtccg gtaccaatct tcatcacgca gtaggagata aagcccagcg
     6721 cgataccttc agtaatcgag aagctgaacg gcatcatcac agcggtaata aacgccggaa
     6781 cagattcagt aagatcctgc cagttcacgc gagcaagact tgaggtcatc aacacgccaa
     6841 cgtaaatcag cgcaccagct gcagcgtagc ctggcaccat ccccgccagc ggcgacagga
     6901 agataaccag caggaacagc agaccaacaa ccactgccgt cagaccggta cgaccgccaa
     6961 cggaaacgcc ggaagaagat tcaatatacg ccgtaacaga agaagtaccg ataaacgaac
     7021 cggtcaccga agagatactg tcgacataca gcgcctgctt catgcgcggg aatttacctt
     7081 tctcatcagc cagacctgct ttatcggtca cgccaatcag cgtaccggag gagtcaaaca
     7141 agttgaccag catgaaagag aaaatcaccc ctgccagccc gaggttaaac gacccggcta
     7201 aatctacatg acccacgaca gtcattacgc tcggcggcgc agaaacgatg ccattatagt
     7261 ggacatcacc cagcatccag cccagcagcg ttgtcaccac gatggaaacc agcaccgccg
     7321 cgtgaatatt gcgcgaggcc agaatggcga tgatgaagaa gccgaggatc cccagcagca
     7381 cgctgtgaga agtcagatta ccgatgctca ccagcgtttc cgggttagcg acaatcacac
     7441 ctgcgttttt cagccccatc atgccaataa acagaccgat accactggtg atgcccacac
     7501 gtaggcttac cgggatgttg gctatcatcc agtagcgaac gcggaaaatc gtcagtaaca
     7561 gcagaccaat cgcgccccag aagattgcgc ccatcccgac ttgccacggc aagcccatcg
     7621 cctgtacaac gacaaaagcg aagaacgcat tcaggcccat tgccggtgcc agtgcaactg
     7681 gcaggttagc aaacagcccc atcataatac tgccgaatgc agcaatcaga caggtcgtga
     7741 cgaagacggc gctggtatcc atgccagcaa cgccaagaat ttgcgggtta acaaaaacga
     7801 tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac ttcggtccgt gccgtcgtgc
     7861 catgttcgcg cagtttaaac acgcgttcca gcatcccctg gccagaagcc tgggttgtgt
     7921 gttgttgact cattatctat ttccgaacaa ggagggaaaa tccgtcgcta tcgtatacca
     7981 aaatgagaca atatggacgg ctgaagataa ctttttttca ttgtatttgc ttatacggca
     8041 acgattgcgt catgcaaatt ggcatgacga aaacgttaaa ctaacgataa aggaaagaca
     8101 tgtcccagat agaagcggta tttttcgact gcgacggtac gctggtcgat agcgaggtca
     8161 tttgctctcg cgcctatgtc acgatgttca gggaatttgg cattcacgtt gacctggaag
     8221 agatttttac ccgttttaaa ggcgtaaaac tctacgaaat catcgacatc atcagtaaag
     8281 aacagggcgt gacgatggtc caggccgatg ccgaacacgt ttaccgcgca gaagtagcac
     8341 gtctgtttga taccgaactt gaggacatcg caggagcgaa cgaattgctg gcaagcatta
     8401 acgtgccgat gtgcgtcgtc tctaacggtc cggtcagcaa aatgcagcat tcgcttggta
     8461 agctcaacat gctgcactac ttcccggaaa aactgttcag cggctatgat atccagcgtt
     8521 ggaagcccga tccggcatta atgttccatg cggcaaaagc gatgaatgtg aacgttgaaa
     8581 actgcattct ggtcgatgac tccagcgcag gtgcgcaatc agggatagat gccggaatgg
     8641 aagtgtttta cttctgcgct gacccgcaca ataaaccgat cgttcacccg aaagtcacca
     8701 cctttaccca tctttcgcaa ttaccggaac tgtggaaggc gcgtggttgg gatattacgg
     8761 catagttatt cacactccct tcacttactc cacttaaatt ggcacgcaaa ggtaagtaaa
     8821 gggagtttga tatgtctgtt tcacgtcggg taattcatca cggactgtat tttgcggttt
     8881 tagggccgtt aattggtgtt ctgtttcttg tcctctacat attcttcgca aaagaaccgc
     8941 tgattctttt ggtaataata caggtacttc cattgtttct cttaatgtcg ataactacgg
     9001 gagctattcc tgcaatgtta actggcgtaa tggtcgcctg tctgccggaa aagatcgggt
     9061 cacagaaacg ttatcgttgt ctggttggcg gcataggtgg agtcgttatc accgagatct
     9121 attgcgctgt tatcgtacat attaaggaca tggcttcctc agcgttattt gaaaacattc
     9181 tttctggtga aaatctcgtt gtccgcatca tcccagcatt gctggcaggc gtggtgatga
     9241 gcagaatcat tacacgtcta cccggattgg atatttcatg tcctgaaaca gactctttaa
     9301 gctaagcgga atgttttatc tatttgtcat taaatgttgg agaacaggcc ggagcgtaat
     9361 tcacacatcc ggccttattt ttaagcttaa ccgagcgcta attctgcggc agcggcttta
     9421 tcagcgatta ccatcagcga aggatgcagc tgcaacacgg aagctggaac atcttcggta
     9481 accggacctt gcagaacatt tttcagtgct tgcgctttac ctgcgccact gacaattatc
     9541 agcaagtttt tcgccgccat gatgcttttc ggccccatcg tcacgtagct gtcaggcact
     9601 aatgaaaagt cgccgcccaa ctcgccatgc gccacaatat caaccatctc tccctggatc
     9661 gggaactcca cggtttgctc atggaaatgg gtcgtatttg gcagattgcc acaaaaatga
     9721 ccatctacac ctaatcccaa caccaccaaa tccaggccgc cttcacgcgc cagtttctga
     9781 tcatgctcgc ggtagttatc aatagtgagc ttctggatat tctcttcttt gatccccgca
     9841 ggggtgaaaa acagattacg cagattggta atcgttacgc cttccccttc tttaccgcga
     9901 aatggaattt catcgaaatt gtagaaatag cagttatcgt accagggctt acctttaacc
     9961 agagtgatga gatattcgta catgcctttg ggcgtgctac cggcggtaat tgccaggtta
    10021 acgcgacgca tctttgacat ataaccaagc agatgatgcg ccgcgacacg gctcatttcc
    10081 tggtaatctt cggtaatgat taatttcatc ttaatgcctt atattatttg aataatttct
    10141 gtacgaaggc tgcataagca ggccgccaga catccatttc atggttcaga cccggatatt
    10201 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
    10261 tgccggttac aacatcttta tccccaacaa ccacagtaaa attacgtagt tgctggttaa
    10321 tagctgccgg ctcgttcagc cgggcagcga ccccttcatc tggtacggtc gtcgtggtaa
    10381 caccactgaa tgtggccagc cagccaaagc tttccagatg attcattccc gataccagtg
    10441 cctgataccc accttgtgaa agccctgcca gtgcgcgacc atcggcatct ttacggacat
    10501 taaaacgctt gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag
    10561 catttaacgg ataaaagact ttacgtctct cctgaggaac gaaatcttcg ggaataatgc
    10621 ccttcgcatc ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag
    10681 ccagcaggtt atccatgatt tgcgggatac gcccctgatc aatagcggaa cgtccggtat
    10741 caccaaagcc gtgatagaaa tagagcactg gcaaaggctc gcctatgccg gtgtatcctg
    10801 gcggggtcca gacatacatc tgacgttcgg attgcaatgc gttggagtgg taagttatgg
    10861 cgatcaaatc accatgcaca acagaacgtg tatccagata actgcccggt accagaatca
    10921 tactggagtt aacctgacgc tgaggcttag tcattgccgt gcctgtatca atgctgcgta
    10981 caccatcaac attgaaaaaa tactcgtaca gattgccttt caggacaggc gtgcgccacg
    11041 accagactcc cgcttcatct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca
    11101 ccactacgga gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg
    11161 tattgacctg agtcacatac tgtttcaccg gaatagtggg tgccgggctg gctggcatat
    11221 ccgctgctat ggcccattga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa
    11281 tttttatatt catcgtaata ttccttttat taagttccat taccaccaga tttcagcctg
    11341 ggcaccgaca gcaaactggt cgtctttatt gtcttcaaag gtgaagccat ccagttcatt
    11401 ttccagagct ttgatatacg tagcgtagaa acgaatttcc gggcgcgagg tcaacatact
    11461 ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagattcat tatatttatt
    11521 gctattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata
    11581 ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat cagaatgtgc
    11641 gcctgtttcg tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat
    11701 aaaatgatcg ccgatataag cttcgccctg cgaagtcaga cgaactgcag ttccgcctgt
    11761 atgatcacca taataacgac cattaaaggt agtaaatgga cttgcaccag cataacgacc
    11821 aaagttactg gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccctt
    11881 atcaaatttc tgcgttaaag atgtaccaaa catccaggta tctttccagt cataataccc
    11941 gttattatcc tgattatctt tttccgatgc gctttcattt gctgagacat aacgaccgct
    12001 caccattaag gtggctttat cccataacgg gatatcttta tagcgtaaat caatggtatt
    12061 ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcacgaac
    12121 cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta cacctgcggc
    12181 agcatcggta cgctgcgttt tccagtcaag catctggatt tcaattttcg gcgcaccgtg
    12241 tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacata
    12301 catatcagag aactgcataa agttctcgcc accggcgtta tcgccaaacc agccagtaga
    12361 gtactgctga cccacgttac catccatcat cacgatggca tcaacccgtt tgccgttttc
    12421 gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg
    12481 gcccagagaa ccaattgccc atgatttagg tgaaccatga gaggctgttc cccaaccaga
    12541 acggtagtag ccgttgtaac taaagccgat ttcatctttc acaaatttac tgaaatcttt
    12601 cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttaac
    12661 cagtacagca tcaggctttg cggcacgact ggtcgggaat ggattggcgg catacccttg
    12721 atcattcgta cttacgctac gattcaccgt cgctggcgta tactttttct tctcttcatc
    12781 tttatatttt ttcagttcgc tttgcgtttc tcttaacgcc ttttctaata gctcaaggcg
    12841 ttgttccact gttaatgatt cagccaatga ttgtgcggaa aaggctaacg gtgccattaa
    12901 taagatggca gaggtaataa tatttcgtct aaacataata tccctttatg gtgcaaagaa
    12961 agaattaacg catcgcatcc agactgttct gaatgcgacg atatttaagg tgctttattg
    13021 gttatttttt taatgatagc ccccgagttt tgatcacttc tgcataccat tcgaagcttt
    13081 tcttgcgcgt tattgtgagg cttccttcac cattatcatc acgatccaca taaataaagc
    13141 cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg
    13201 tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtaca aggtgatcgt
    13261 ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cacttcaacg ctgtcttttg
    13321 cgcctaatcc gttctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta
    13381 acagaatccg taatccaacc ggatcaattt gccaccccca ctctgaactt ttcagatgcg
    13441 gatttgggat catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg
    13501 aaacacaacc ggtcatgtaa taactaaaag agatgaaatc tacggtatgt tttaaatctt
    13561 ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat
    13621 agccgggata ctgaccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt
    13681 ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa
    13741 gcatattgcc aattttcgct tcggggatca ggctatgaca ggctttaact gcccgcgcac
    13801 tggcaaccag ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca
    13861 gccccacgcc cgtgaatggc gcgtgtaatg acatgttgat ttcattaaat gtcagccata
    13921 acgcaacttt atgttggtag cgggtaaagg ccgtgcgtgc gtaatgctcg aagtgatcga
    13981 tgaccgcccg attagcccaa ccgccgtagt ttttcaccag cccatatggc atttcgtaat
    14041 gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgctcgt
    14101 aaaatgctaa ccccgcttca ttcggttcgg cttcatcgcc ctgaggaaaa attcgcgccc
    14161 aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg
    14221 ggtaacggtg ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg
    14281 gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat
    14341 cttcctgcca ggcgccttcc atctgattgg cagctgttgc accaccccaa agaaatgttt
    14401 ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat aacagcggct
    14461 caccagcgtt tatctgcgcc gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac
    14521 tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag
    14581 aaatcagccg atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt
    14641 tgccgtccag ttttacggtg tcgataccga catgaatcag gatctccacg ccatcatctg
    14701 actcaatgcc gatggcatgt aatgtggcga acaacgaagc aattcgaccc gccaccggag
    14761 aacgcacttc accaacagag ggcagaatgg caataccttt acccaacagg ccgctggcaa
    14821 acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac
    14881 cgccctgctc aggtgtaata acctctggtg ttttctcttg ctgggcaact tgcgctgact
    14941 gactcttagc ggtgatgaaa tgaaacatca ccgtaccgac aaatgcgcaa ccgatggcaa
    15001 tgacaccgcc aataacgctg gcccatacgg tgaaatcaat ccccgttgac gggatggttt
    15061 gcatgaaggt gaaaatacct ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
    15121 tgatggtggc ccccaatgcc ccacttatgc aggcgataac gaaggggtac ttacgcggca
    15181 gattgacgcc atataccgct ggttcggtga taccaaacaa acctgtcaac gccgctgatc
    15241 ccgccaccac ttttttctgt gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
    15301 cttgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
    15361 agttattgat aattaaaggt atcaaacccc aatgcagacc gaacatcaca aagatttgcc
    15421 agaagccgcc cattaccgcg cctgcaaatg caggaaccgc ctgataaagc cagagataac
    15481 cggcagcaat cagttcactt atccaggttg atagcggccc caccagcaga aatgtgatgg
    15541 gcgtgatgac catcagacat agcaagggtg tgaagaaatt tttgattgcc gaaggtaacc
    15601 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
    15661 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccagcgcat
    15721 ccactttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccgccaa
    15781 tcaccatggc agtaaatgga ttgccgccga agcgtttccc tgcggtgtag cccaggatta
    15841 tcgggaagaa ccaaaacaag gcatcactgg cgctaaataa aattaaataa gtaccacttt
    15901 gttcggtcgc ccactgaaaa gtgagtgcca gagccaacat acctttcagg atcccggttg
    15961 ccgccatcaa accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
    16021 acagattatc tttatcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
    16081 cgctgttaac cgccaggaag acatcggcca catggttacc tatgacaacc tgaaactggc
    16141 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
    16201 tgctttcatc ctttaactta aaacgtaatc gcgttgcaca atgcatcaga ctcacaatgt
    16261 tatctgcgcc cccgactcct gcgactattg ttctggctaa ctccgtcata acttgccctc
    16321 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
    16381 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttattc agtaataata
    16441 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcaggt
    16501 gagattttac gttgatactg caaaccaata aaaatggcga tccgctccgc acattgccat
    16561 gcttgcgggt aattttgctt tactgcttgt tgtaatgatt catcactatc gttaatcgat
    16621 gcatgttcaa gaatacgcca ggataaaaat ttcagatgcg taaccagtcg ctgataactc
    16681 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttctcgc
    16741 attaattgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
    16801 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
    16861 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagtcgctg gatatcccac
    16921 agcagcgggt tgggcagtag cacgttttgc tgaaagcgtt taatcgcaaa ctggcaatgg
    16981 tcagttagcg agatataaat actgtcctgc aactttccca gacgctcctg cgccagagag
    17041 ataatacgat cacaggttgc catcacctca agaggcatat gacttaagag ttcgcttaat
    17101 cgcccgttca gttcatgact gctcaaggca tactcttttt ctattccact tgagttaatt
    17161 ctttcgcctg ggcgtttttg aaagccaatt ccgcgcccca tgacgacttt ttcccgctgt
    17221 tgatcatcaa taaccaccac aacattattg ttgagaattt tggtgatttg catgttcata
    17281 gcaaggacct tttttataaa caaaaaaacc cgacttcacc agtattctct ggttatgtca
    17341 ggttttgcct gcgaatgcag taacaatcca gtcattaatt aatggttttt ataacgaaca
    17401 tccaggttcg aaaattaatt tagttgcgtg cagagaaaat agcaatgctc tatttataaa
    17461 agtatgacca tgctcgcagt tattaatttt gtgtaatttt aggattttat aaagttatat
    17521 ataacaaatc ccaataattg aattattggg atttacttgg tgaattattt atcgctatct
    17581 ttttctgcca gcagtttatc gagctcgtca ccaccgacat gacggaaatc ctgccccttc
    17641 acgtagtaga agataaactc acaaatattc tggcagcggt cgccaatacg ttcgatagaa
    17701 cgcgcacaga ataacgcggt aagtacgctc ggaatggtac gcgaatcttc catcatgtag
    17761 gtcatcagtt gacgaacaat accttcgtac tcctgatcga cttttttatc ttcacgataa
    17821 atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt ccagcacgtc gtgcagcatc
    17881 tggatggtat gacggcccag cgactccaga cttaccagca atggctgatg ctgctgggag
    17941 aatttctcca gtgcagtacg gcagattttg tccgccacgt cgccaatacg ctccagctcg
    18001 gcaatggttt tactgatcac cataaccaga cgcaggtcgc tcgccgtcgg ctgacgtttg
    18061 gcgataatgc gcacgcaggc ttcatcgatc gccacttcca tcatgttgac gttcttgtcg
    18121 ccttcgatga cgcgcttcgc cagatcgctg tcctggttat gcatcgcggt gatcgcatca
    18181 gaaagctgct gctccaccat gccgcccatg gtcatcacct gcgtgcggat gctttccagt
    18241 tcggcgttga actggccgga aatatgttta ttaagattga gactgtccat aacgcactcc
    18301 tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg tttcttcgct ggcttggtga
    18361 acagatcgtc cgtgttgctg aactcaatca attcgcccag gtacataaac gccgtgtggt
    18421 cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat caccacggtg taatcctgct
    18481 tcagttcggt gatcagctct tcaatacgcc cggtggagat ggggtcgagc gccgaacacg
    18541 gttcgtcgag cagcagtact tccgggcgaa tggcgatacc acgcgcaatg cacagacgct
    18601 gttgctgacc accagagaga gagtaaccgc tctggtgcaa tttatctttg gtttcgttcc
    18661 acaatgcggc tttggtcaat gcccactgca cgcgctcgtc catgtcagca cgggagagct
    18721 tctcaaacag acgcacgcca aaagcgatgt tgtcgtagat ggacatcggg aacggcgtcg
    18781 gtttctggaa caccataccc actttcgcac gcagcagggc gatatcctga gagttggtca
    18841 ggatgttgtc gccatcgagc agaatttcac cttccgcacg ctgttccggg tacagttcaa
    18901 acattttgtt gaaggtacgc agcagcgttg atttaccgca gccggacggc ccgataaacg
    18961 ccgtaacctg gtttttggcg atatccaggt tgatgttttt cagggcatgg aatttgccgt
    19021 agtagaagtt caaattacga acctgaattt tactcggggc agtttcaacc atactcattg
    19081 caatcttttc ctcatcgtgc ctgatgcact tcgcgaatca ggcttaccgt aggccggata
    19141 aggcgtttac gccgcatcca gcaaagtgtt aaccgtgttt attcttcgca aaaacaacgc
    19201 gcgccagaat gttcagcagc agtacgcaca gggtaatgat caacaccccg gcccaggcca
    19261 gttgctgcca ttccgcaaac gggctcatcg caaacttaaa gatcgtcacc ggcaggttgg
    19321 cgatcggctg catcatgtcc gtgctccaga actggttgga gagcgcagta aacagcagcg
    19381 gtgcggtttc accagcaata cgggcaatcg ccagcaggat accggtcatg atcccggaca
    19441 ccgacgcttt cagcgtgatc gaggcgatca tcttccgctt cggcgtaccc agcgcataag
    19501 ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt ctcggtggtg cggataacaa
    19561 tcggcacctg caacagcgcc agcgcaatca cgcccgccca gccggagaag tgctccatct
    19621 gcgccaccac aatggtgtaa acaaacagac caaccacaat cgacggcgca gagagcagaa
    19681 tgtcgttaat gaagcgaatc acttctgcca gccaggattt acgaccatat tcagccaggt
    19741 aaatccccgc cataatgccc agcggcgtac cgaatacggt agcccacaaa attaacaggc
    19801 cgctacccgc cagggcgttc gccagaccac cgccttccgt attgggtggt ggcgtcattt
    19861 cggtgaacag cgccagcgac ataccgtcga taccgcgagt gatggtggac attaaaatcc
    19921 agatcagcca gaacagaccg aaggccatcg tcgccattga gagcgtcagc gcaatacggt
    19981 ttttgaggcg gcgacgcgcc tgcattttgc ggcgggattc agccagtgcc gcagtggttt
    20041 gcatttcaac catagccatt agcgtgcccc ctcattctta gccaggcgca taatcataaa
    20101 cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg atcaggccca gttccatcag
    20161 tgcagcgacg tgcagaccgg attccgcttc cgcaaattcg ttggccagcg cagaggtgat
    20221 actgttgccc ggcatataca gcgaggcgct gtcgagttgg taggtgttac cgatgataaa
    20281 ggtcaccgcc atggtttcac cgagcgcgcg ccccagcccc aacataatgc cgccgataac
    20341 accatttttg gtgaacggaa gaacgatacg ccagataact tcccaggtgg tgcagccaat
    20401 accgtaggcc gactctttca tcatcaccgg ggtttgttcg aatacatcgc gcattaccgc
    20461 tgcaatgtac ggaataatca tgatggcgag gatcacgcct gccgcgagga taccgatacc
    20521 aaaggccggg ccggagaaca gcgcgccgac aatcgggata ttcgacatga tattgccgac
    20581 cggctcctgg aagtaaacgg cgaacagcgg cgcaaagata aacaggcccc acatgccgta
    20641 aacgatactt ggaatggcag ccagcagctc aatggcgata cccagcgggc gtttcaacca
    20701 gccaggcgca agctcggtca ggaacagggc gataccaaaa ctcaccggga cggcgatcag
    20761 cagcgcgata aacgaggtca ccagcgtacc gtagatcggc accagcgccc cgtagatatc
    20821 gttcggcgca tcccactctt tggtccacag gaaagccaga ccaaatttct gaatgctcgg
    20881 ccaggatgag atgatcagag agacaataat gccacccaac atcaatagca caatcagcgc
    20941 cgccagtttt accagcacgc tgaaaattat gtcgcccttt ttacccggtg ggttaaaagc
    21001 aggcttggtt gcagccataa gttactcttc agttaaacgc gtttacaaag tcattttgta
    21061 atgccggatg cggcgtaaaa caccgtaccc ggcctggagt tttattagta cagcggcttg
    21121 ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa cctgttcaac tacgctatcc
    21181 ggcaggctgg cgtaatccag gtcgttcgcc tgtttagccc cggttttgta tgcccagtcg
    21241 aagaacttca acacttcggt gccttgttct ggtttcttct gatctttatg gatcagaatg
    21301 aacgtggtag aggtaatagg ccatgcatct tcgcctttct ggttggtcag atcctgagcg
    21361 aatgttttgc tccagtctgc gccttttgct gcattagcga agttttcttc ggtcggactc
    21421 accggtttgc catcagcgga gatcagtttg gtgtacgcca ggttgttctg cttcgcgtaa
    21481 gcatattcaa cgtaaccaat tgcacccggc agacgctgaa cgaacgcggc gataccgtcg
    21541 ttacctttac cgcccagacc gatcggccat tttacggtag agccagtacc aacgttgttt
    21601 ttccactctt cgttcacttt cgccaggtag ctggtgaaga cgaaggaagt cccggagcca
    21661 tctgcgcggc gtactacagc aatgttctgt gaaggcagtt tcagacccgg attcagtttg
    21721 gcgatggctt catcatccca cttcttgatt ttgcccaggt agatgtcgcc gagggtttta
    21781 ccatccagca ccagttcgcc agacttcaga cctggaatat taaccgccag caccacgccg
    21841 ccaatcacgg tcgggaactg gaacaggcct tcctgagcca gtttttcgtc agacagcggc
    21901 gcgtcagagg caccgaaatc aacggtgtta gcgataatct gttttacgcc accggaagaa
    21961 ccgataccct ggtagttaac tttattaccg gtttctttct ggtaagtgtc agcccatttg
    22021 gcatacaccg gcgcagggaa ggttgcacct gcacctgtca ggcttgcttc tgcaaacaca
    22081 gagaaagcgc tcatcgataa ggtcgcggcg acaacagttg cgacggtggt acgcataact
    22141 ttcataatgt ctcctgggag gattcataaa gcattgtttg ttggctacga gaagcaaaat
    22201 aggacaaaca ggtgacagtt atatgtaagg tttatgacag ttttatgaca gagagagaat
    22261 gtcttcagtc cgatttaaat aagcgtttat attcagttaa ttacaaatat taataacgaa
    22321 gagatgacag aaaaattttc attctgtgac agagaaaaag tagccgaaga tgacggtttg
    22381 tcacatggag ttggcaggat gtttgattaa aaacataaca ggaagaaaaa tgccccgctg
    22441 tgggcggaca aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt
    22501 agggaagagt gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt
    22561 aaaaatgaca aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac
    22621 ctgccgctca gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc
    22681 caaattaaat ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg
    22741 gacaggtgat tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga
    22801 cattatgcat tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac
    22861 aagcattacg aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa
    22921 ctatcctcca cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc
    22981 agcggctcgc tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt
    23041 gatgaagctg cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa
    23101 tctacggtaa gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac
    23161 ttttgggctt ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac
    23221 catgaatggt cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac
    23281 tggctcatat ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg
    23341 ctactcaggg ctacataaag atatttatct acttttactg attgataagt agcatcagtc
    23401 catccgcagg actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg
    23461 tggtcttaac atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt
    23521 gtaatagatg cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga
    23581 tgcctgcttt ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat
    23641 ggcggcataa tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca
    23701 tccacgccgc ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg
    23761 gccctacgcc ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt
    23821 ttattgctgg gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt
    23881 catgcacagt gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt
    23941 cattaatgcg caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca
    24001 taacctgcaa aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct
    24061 tgcttgcagg gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa
    24121 aagctagggc atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta
    24181 agctggataa aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt
    24241 gaaacagaat aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac
    24301 ctttttgtcg catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct
    24361 ccttgtcttg caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct
    24421 gttttttacg cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat
    24481 taaaatgtta tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca
    24541 tgcacgattt gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt
    24601 gattatatta attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa
    24661 atgttaacct ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg
    24721 gagttatcgg gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta
    24781 aagctattac ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag
    24841 cattacccaa ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga
    24901 acgtattgat ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc
    24961 atgtcattgt ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac
    25021 tacatatatc tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca
    25081 gcaattttag gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa
    25141 tctggcaaaa gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta
    25201 ccaaaagccg cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt
    25261 tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt
    25321 gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc
    25381 ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc
    25441 gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta
    25501 gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt
    25561 ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag
    25621 acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc
    25681 ggcattgacc tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac
    25741 tggtactggt tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct
    25801 tcgcggcgcg gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta
    25861 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
    25921 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
    25981 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
    26041 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
    26101 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
    26161 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
    26221 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
    26281 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
    26341 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
    26401 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
    26461 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag
    26521 gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt
    26581 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca
    26641 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gagagctggg gaagactatg
    26701 cgcgatctgt tgaaggtggt tctaagcctc gtacttgcga tggcatcggg gcaggcactt
    26761 gctgacctgc caattgtttt agtggatgaa gctcgtcttc cctatgacta ctccccatcc
    26821 aactacgaca tttctccaag caactacgac aactccataa gcaattacga caatagtcca
    26881 tcaaattacg acaactctga gagcaactac gataatagtt catccaatta cgacaatagt
    26941 cgcaacggaa atcgtaggct tatatatagc gcaaatgggt ctcgcacttt cgccggctac
    27001 tacgtcattg ccaacaatgg gacaacgaac ttcttttcca catctggcaa aaggatgttc
    27061 tacaccccaa aaggggggcg cggcgtctat ggcggcaaag atgggagctt ctgcggggca
    27121 ttggtcgtca taaatggcca attttcgctt gccctgacag ataacggcct gaagatcatg
    27181 tatctaagca actagcctgc tctctaataa aatgttaggc ctcaacatct agtcgcaagc
    27241 tgaggggaac cactagtgtc atacgaacct ccaagagacg gttacacaaa cgggtacatt
    27301 gttgatgtca tgtatgacaa tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc
    27361 cgaattccag actatgtcga actagataac gcggaggagc tggccgacgc aatcattcga
    27421 gaggttgagt taaaggaggc atggctcaca agggtcttgg ggaactcagc cgatcctgac
    27481 gcttctgtgg gcaagtggga ccggcgccta gcgcagtgcg gtggtattga tgagatgtca
    27541 agtggggtgc aggcaacata cttcagtcca aacggtcagc tggtcggctt agtggcatac
    27601 attgatgccg aatggatgga tgaatagctc tgacgttacg attcaggtga gacaaccaga
    27661 tgggcattgc gtacaagctc agttgccacc gatgtggcta cgagtctgat cttctttacc
    27721 taggccaagg tatggcgatg cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca
    27781 atctaacgac gatttctata aatgaaagct acggagtatg ctcactttgt ggggctaagg
    27841 atcgtgtgat atttacaagc tatcagcatg aggcaaggcc aagaaatccc tgctatctag
    27901 agagaagttt caaatatcaa tgcccaagat gccatgcctt cagtatggag cctccggatc
    27961 taccggagat gctttgggac taatgctccg ctttgcctcg ggtatccgtt cgacctatgt
    28021 ttaagaggaa acgcacgtgt ctgccgccaa agctcttgcc tatgcacttt gtgacaagtt
    28081 ggaaagcaaa aaagttgatg aagtcgtacg aaatcttgtt tctagcggct ggaatattag
    28141 ggaagtggcc tgggaagctt cgtcctttga tcatgaaatg ccctactttg acacggggcg
    28201 ggacttcgat attggatttc ctgacacgct gtcatccaag attgagtttt tgaccgcgaa
    28261 tggatcaatc gtccaagcac gtgctcgcct gcacttcaag caggctctaa tttttgccaa
    28321 ggcgtccaaa catgtcgccg cgctcaagaa ctcattggat tactactatg gatcgtctgt
    28381 aatcccccct caaaaccacg gaggctattt gtagaaaatt ccattaatat gaactcaagg
    28441 tgttccctac ggacgaatca gcgatgaagg tggtgtacct ggctatccag gcggcgacca
    28501 agaaatggac gatgccgatc cgcaactgga agccggccat gaaccgtttt atgatcgaat
    28561 ttggtgaccg tttaaatggc cacctttaac tgaccggcaa aagcacttac acaaaagggt
    28621 ttacaggctc tttcagtcat tcctttaatg cgtaaattgc tctctaacca ctctctcagc
    28681 ccacccttcc atatcctctc tcgatacttg cagctccccc aacccctttg gatgattcac
    28741 ccctctatgc ccgtcttgtc catataattg atcaaacaaa ctatttggcc aattcaatga
    28801 gctcttcacc actccacgcc gtatcttttc aaaatcattc cgccatgttt cgctatcaac
    28861 gccactcaac actttggtag aaagcatttt caccccatcc gatgcatcca cctctagcaa
    28921 tacaaattca ctgccattac gttgcacccc aacggcttta atcactcttg gtgagccatc
    28981 tttacacaga tgtaatcggc tacgcccaac ttttggcaaa accaaggttt cttcaaaaag
    29041 cacccgacat gcaaatttag tttttagaat tgaaagtagc tcatcaaaag cagcaaatcg
    29101 attagcaaaa atagagttgt aattggttgc atcctgcttc ccacccacat ctgccgctgc
    29161 taggacacca cctaagtgtg gctcatcagt actaaccaaa ttactatttt cacttgttgt
    29221 tgcttcttct ttttctaaaa tagtttggct actttttctt gccctgcgag atttttcaat
    29281 tcgactaggt ctactaaaac ttatccatgt cggctcagcc tctatgactc gtgtgtctgt
    29341 gtctatattg gctgtttcct catcgtccaa ctgtagatgc tcatcatcaa caactgactc
    29401 tgttgaaacc gctatgtgct gcgtactacc atcacctgcc tccttttcct gaaaagaggc
    29461 atggctaata gctgttgtgc taggaagcat agcgttgatt tctaaaccta ctatttcctc
    29521 aactaaataa tccttatcct cgttagaaga acgtcctttt acatgtaatt tccaaccctg
    29581 catgggcgga gggtcaaagc taaagcacca gctttcaacg ccgttcttaa tttctctatt
    29641 ttgttgatag tgcctaaaaa tactttcata cgaatccata acatcttgat ccgaaaacaa
    29701 ccaaaccaaa agctgcacta cggccgactg ctctaacgcc cctttaggaa acgatgaact
    29761 gggtaagatc cttatctcta aatgatctcg ctcaacctca tactgaacgt cgaactcttg
    29821 ctgtaaagcg gtactgctca aacagcttcg acagaaatag gagttaatta aaaataatga
    29881 ccgagctagc tctaactgtg ggatgtgata acgagtcttg ctatatggtg tttcagcttc
    29941 gtaaataaat gaactctgcg ctccatcctt gttgcgtacg gaagcaaagg acgagagatt
    30001 aggaaaatcg attattttgg ctcgttgaag atccgatgct tcaaactcaa tcacacgatc
    30061 cgccttattt atttgctttg ttgtactgtt aaccacctta gctcgactta ataaaggcaa
    30121 atgagtaaat cgagtccatt tccgttcttg cattggatta aaccaaacaa aaatacgcca
    30181 ctccttatga cccgagttac ggaataaatg accaatatgt acaacctgca cattgtcatt
    30241 aaatgtagct agcctaacca ctcactcttc cccataaacc atttccaaga atctttgtgc
    30301 ttcctcagtt atccgctctt tagataacgt tgcacttctt aataatcgcc agcgcctaag
    30361 ctcaacatct tcctgtttaa gcttaataaa agcttggcta atccgtctaa tttgataatc
    30421 ttccacactc tctgagtaac gctttaagca aagcgctacc aaaggcagtt tctgtagatt
    30481 ttttgcaaga gaggttccgt taggagtttg ctttaacagc cagctcgatg tcgctcttgg
    30541 gtgatcaagg ctactatcta gacgctttat gattcttaat agttgtcgta cagcaattcg
    30601 atctctttgg ttccaatcaa ctctaggggc gggtgccaga cgctcttgtt gatgctgttg
    30661 attccagtga actagccaat ccctgtcatg tcggtaaagc caagcgtata gcaccccacc
    30721 ctctaaagac tgtcttgccg ccttaattcc ttggtattta tgcactagtt gctgccagtc
    30781 tttatgctta acagataaat cttcagagtt aggttgcaca gactggctaa caggtcttgt
    30841 cgttatagag tgctcagtaa gagcacttgc ctgctgtagc gcttctataa ccgttagttt
    30901 tggcaataag gcttgccaca caatactatg ctgtaaataa ctaaaggctt ttctatgctt
    30961 acggaatata cttttcagcc aacacgtgtc cttgttctct gccaacttta aatccagttt
    31021 ctctagtgcc tcatcactaa aagtctgcct cactctctcc gccaccaagt catgacgaat
    31081 gtgcttgctt ttggttagcc ctagatcctg cgctaagcgc tgataaaaca gcgtccactg
    31141 ctcaaggctt ggggaaagct cttgcgctcg tggagcatct aacagagggg ctatataagc
    31201 agctagtgct gttaattgag atagggagtc tttggggtag tctgaaagca gctcagtatg
    31261 acccaaagcc caaaattgat gtcggtgatc atctacagct ctatcaaaga agactaaagc
    31321 accgtgtttt ggacaatatg gcaaagcggg caaataccaa tctcgttgcc aaaaggcttc
    31381 cccataccta tttagctgaa gagcaacgca atcagggcag tatctaaagc ggttatcgct
    31441 cttaactcta gaagcagcga ctcctagcat taaatgcacc gcaccttgcg cttggtactc
    31501 cattaaccga atagcttcgt ctcggcgctc cttgcctaca aacggagcat ataaagggaa
    31561 taaggtatgc tcataaataa gctgctgaac agcgtaacgt cctgtttgat gtagatgtct
    31621 tgctatcaca cctaaatgcg agggcagacc taaggtagcg accaccttgc ggttgccata
    31681 cacctcatcc aacagctgct taggactaac aatcccttga taaacgcctg cccgtgcaat
    31741 agtgctataa atcagctcat tcgagtacgg aacaggaaag tttctcatgg ctaacctgct
    31801 tgtttaaata agctcgccat atccactatt acccctttcc cttttaaccg ttcatgcatg
    31861 gttttttcag gttggcgttg tgaatagata taacgtaaat ccgtatcagg caagctatcc
    31921 cagtcgctgg gcttgactac ctttatagcc gaaacctttt tactcttgga gggcttttct
    31981 agttctgaca ctaccgtttc gccttccatc aaccactgca aaacaagagg cagtaacttt
    32041 tgtcttgtca tcgttggatt ctggctaaac gcttttttaa tagtgggaat taacaggctt
    32101 gaatcgtaat cctctttcag catcagatat aaatgacgct gatcttcggt atctaactct
    32161 tgaagggctt tttcttctgg tgtttgttct tgtatcgctg cgatatctag ctgaagttgg
    32221 attaaccgtt tatcaatctc gggaacgact agatcagaat aacgagcaat gcgttctggg
    32281 atacccgagc gtaatgcctc tagcatgggg tgcacaggct ttaactcatc ttgatacact
    32341 tgccgcaata aaccagcggt aatacgctca ttgcctaaag ctagcgcacg gagctgagcg
    32401 agtacaaaaa gttttactac aatgtccatc actccttggc ttagctcata ccacacatca
    32461 cggacctcat ccgataacag cgcatctttg cgttgtaaaa gctgtaattg ccaaagatta
    32521 tccgtaaaag cgatccactc ttgattgggc tttccacgtt gcgtttgttg tataggatcc
    32581 cagaatatag ctccaaaccc tgcccctcta cgtgcagacc gcaaatcagc ctcaaaaatc
    32641 tctcgtgctt taggggtacc aatcaacatc actggtacgc caataatatt caccatcgtc
    32701 acaaaaaagt tcagcatctc ttgagatcca cccgaacgag agcggcttaa atgctgaatt
    32761 tcatcaataa ccaacaaccc taaagcatgt gcattggcta tttgcgacat caaagccaac
    32821 atggtttcta taccatgacg ttttaagcca taacgacgct catagttcga gcccaaggct
    32881 cgatccaacg ctctgaaaaa attcaagcag atttctttta gcgaaccatt atgcgagcag
    32941 tctattttca aatacaccac ctgctctaca ttgagttcac gatggtaaat cacctgagga
    33001 tacgtggcta gaatacgatg aagagaggtc gtcttcccac taccagaaca accaattaac
    33061 aataagcttt gtgccgtaga tcgtgcctcc tcaaagcgaa atgtctccaa ctctcccgtt
    33121 tgaacacgct cataaccatt ttgtaaatgc ttttgtaaat ctcctgtttt aggatttctg
    33181 cctacgtagc cacctcgaat catgaccgaa atacgctcac ttagtagcaa atgcgtacct
    33241 aatggctgaa aatagtcatc tggaatacga caaatggtat gagctctgat aacacgggac
    33301 ttttgcaagt cagaggaagt aagctgtaaa gaggatttca gtgatgcagc actattcact
    33361 gactcttgta atggtggtaa ggcctcgata aaaggattat cacgataagc ctctaccccc
    33421 gtatcacgat aaactgcttg aatccgggta gcactcatga ctcatccttt tctggtggat
    33481 cctgaaataa ttcaggcacg tatgtgggta ggctgtaatc ttcttgatca tccgcttcca
    33541 ctgcgttgaa aggaataact ttagcctcat cacctgaaga gcttggcttc aaatgctccg
    33601 cacgtttttt acgctccgag gtcacggctt cttttttatt agttttaatc tgcttaatgc
    33661 gtgttgattt gggctcagta gtactgggcg ttaacttatt cgctttctga atggtttgct
    33721 gaataaacgc ctcaagctcc ctgcgtttag ttaactcatc ctgcttcgca ttggctttat
    33781 tgtgtttttc ttgtgcttgt atatcccaaa cctcccaaaa tgagagacct ttaaactgcc
    33841 gactacgttc cgtcagatta cagcgccaaa atacacggct gccaacttgc ggaaacaaat
    33901 aaatcgtatc aaccagcact gggtcataag ccgcttctaa atgttgaggt ctagctatat
    33961 cagtgctccg ctgcaaccaa ccctcacgca gaatctctga ccccgagtaa tacaaacccc
    34021 acaaattaac gccaaatgaa gaaatagaga cctttcggcg aggcagtaac gctactcgca
    34081 actgctcttg ctccacagcc cttaaactac ctgtacgatg ctgcataccc cattgccata
    34141 gctggacagg aatagacggt aaatctgtag gaaaatcagc atctcgatcg tatttatcca
    34201 tcaccagatg gttatttctg aataagatcg tacgcaaaat aatttgtgtg aactcaaata
    34261 ccgacagaga tgcatctaac ctatagtctg tttcaccatg gcttttgatc cgactgccct
    34321 ctacaatgcc aggtgcaaag gacttaaact cggcttgtag tgttctaaaa gtgctttcca
    34381 ctatgccttt agcatcgcca cgtctaggtg gagcactttc cactcgcaca ttaaaactag
    34441 aaactaaggc ttcgacctga tgactcatta attcgccacg gtccgctagc aacacatctg
    34501 gcaaacctac acacggccag tctgagctac taatctcaat atcatgctgg gcacaaatgg
    34561 ccgttttgtc agagcaagca tttacaaaag cctgcatcgc caccacataa gacggatttt
    34621 caaagccgat ataaaagccc gtgatcatcc gactaaacac atcaatcaca atgtaaagcg
    34681 ttggtcttcc tatgattttt tggcgatcat gatgatccac taaataaata tcagcaatcg
    34741 tggcatcaat ctcataacga ctcccagggc ctaacgcctg agaagtggct gtactactta
    34801 agggtcgtac gtctttttta tatacccctg ctttaactct agactttaag cgctgagctt
    34861 tagggtattc tcgatcataa aaataacgaa actgacgtag tgttgggtaa tcctcttggg
    34921 gaatgcgagg aaaatactga gcaaacaagt ccacaaatcg tctataggca acggtggtct
    34981 ttgtaccttt ttgatttaac aggtgctttt ctatggtcaa cctaaaaagg cgttcaatct
    35041 cgggcgttac cttggttcct tcacccttac catattctct ggctcggcca atctttgctg
    35101 ttcctgtcgc tgaacgtctt tcccctggtg caccgctgtt tttgtagtca ggaattaatg
    35161 cattaggcgt ttgaccacgc tgccagtaac ggcgtaacaa cttataaact gtagccttag
    35221 taaccttatg ttcttggacc acatgctcaa cgagttcgct tctgacttta gggtcgaaac
    35281 gatccttact attaataata ggaagaattt ttcgataatc ctcctcgcgc ttctggaagc
    35341 tgacagaatc tacagacggc tcttctaaat caagatgtac ataagggtca tcagttctaa
    35401 ctaagcggcc ttcatctaaa tactgcatta gcaacaactc agccctagct tgtggaactc
    35461 ctttatcagc gcttatttgc atccagacaa cttggccatc ctctatagcc aaaatgcgat
    35521 acggatcatt atcaaatagc acaacctcat taatttgcca catagcgcaa ctcctccata
    35581 ttcactactt ggctaataca gagatctgca cacttatttg ccctgaaaga cttataaata
    35641 ttgaacttaa taaaaccatt tgcggttaag gctcgtatct cactcaatgt tttgcctaac
    35701 tccaaatcat aagcaatatc aacctgctta cagacattga taatgttttc atctcctttt
    35761 tcttgcagga tatgggccaa tggggatagt tgtgctaaaa gctccgcaga aacttcttct
    35821 gttttcactg aataaagcca ttcaatattt tcttttacta cgggatttat ttctttatca
    35881 gtaaaaatga accaaggaat ttgcttttgc tgccaatagc gacgctctag ttctagtttt
    35941 tctaaggtac gctcgtcttg taaggctgct gcaggtttga cttgaatagc aaactgctca
    36001 aaaggaccat ctttgcagtc cactaaaaaa tcagtagaca taacctgatc tacaccacga
    36061 ataacaggat gcttaatacc actatctatt gcaatctgcc tggtatcact aggtaataag
    36121 gggaactgct cgcgtatatc tagcacgctg ctctcccact caagactgag aaaaacagca
    36181 agctctaagt cagatagcaa atgatggact cgtcccgtct tatgagaata aatacggtgg
    36241 gaacgacctg aagaaggaac ttcttgtact gttagccatg gaatatagtc tttaccatgc
    36301 ccttggccac gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt
    36361 gctttagcca taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt
    36421 ttacaactat tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag
    36481 tttaagactt tattgtccgc ccacacccgc ttacgcaggg catccattta ttactcaacc
    36541 gttaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg
    36601 tggtaagcca gtagttgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
    36661 tgcggcatct caataatgtg catattgtca ctgctcacaa aacccgcatc ctgatcggcg
    36721 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
    36781 agcaattcgt tgttcggtgc aacgacgata accggcatat cggcatcaat cagcgccagc
    36841 ggaccgtgtt tcagttcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
    36901 agcttcaatg caccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
    36961 tgatgtttgt cagagaaatc ttctgccagc gcttcaatgc gtttgtcctg agacagcatc
    37021 tgctcaatac ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca
    37081 tccagacctt tcaggcgaga cagtttcgcc accagcatca acagcacagt taactgggtg
    37141 gtgaatgctt tggtggaggc cacgccaatt tccgtaccgg cgttggtcat cagcgccaga
    37201 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
    37261 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
    37321 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
    37381 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
    37441 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttca
    37501 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
    37561 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg gcggtaaata
    37621 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
    37681 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
    37741 aggaagataa agcgacgagt cactggcaac agcgccagct ggtcagaagc gataaagttt
    37801 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
    37861 ggatgacggg agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc
    37921 agaactgcct cacgcagagt accgccttgt ttcagctccc agttcaccaa atgtgcaatc
    37981 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
    38041 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
    38101 tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg
    38161 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctgtacttt accgaggcga
    38221 cgcaggcggg tcatatgacc ttctgcatca acaacggcca gaccggcaga atcatatccg
    38281 cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
    38341 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
    38401 atgcccgttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    38461 cttgtgggcg ggagattatg ttatctcctc atcccatatg accggattac ccggccagaa
    38521 ttactttttc tttaccggac gacgccagcc ttctttttga gtctgcggca cacggctgat
    38581 cgccagggca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    38641 tttgcctact gttaccgggg ccaccagttg agtgtcggaa ccgacaaaca catcgtcgcc
    38701 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    38761 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag ctttcgagcc
    38821 tttacccaga cgcgcttttt tcatctcaac gaagttaccg acgtgtgcac cttccagcaa
    38881 ctcagcacca ggacgcagac gggcaaacgg gccaatagta caggccgctg ccagattcgc
    38941 gtcttccacg acggtatatg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    39001 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    39061 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    39121 cggatcgcgc agcataacac ctgctaacag cagtttttca gcctgttcgg actggtaaac
    39181 gcgctccagt cgggagagtt gcaggcggtt attcacgcct tctacttcgc ttaaacgttg
    39241 cggatgaacg gcgacgattt cacgtccttc ctgatacgcc agcgcaataa tgtcggtgat
    39301 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    39361 gccgttggca atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    39421 atctttgtgc tcaacaatgc cggtaacttt gccgttttca cgggtgatac gtccataacc
    39481 ggtcggatca tccagtttta ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    39541 cagacgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    39601 ttcatcatcg gcaaagaaag gtgcggcctg ctgcatcgca tgacccgtac ccagctgttc
    39661 tgcctgaagc acccagttca ggttgtcgtc tttcagcgcc tgcttaagca gatcgccgcc
    39721 gtgaccatac acaaggtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    39781 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    39841 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    39901 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    39961 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagcgcc
    40021 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    40081 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    40141 tttatatcat caggatctat gtgaacgctt ttcaggacgg gtcaatacgc gtaaaaaaag
    40201 ccagcctgtt gccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
    40261 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
    40321 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
    40381 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
    40441 caggctgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
    40501 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
    40561 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
    40621 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
    40681 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
    40741 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
    40801 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
    40861 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
    40921 ttcatccata cccaggatgg cgataatgtc tttcagttcc tgataacgtt gcaggatgga
    40981 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
    41041 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
    41101 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
    41161 atccgcaggt acgtatactg cctgtacgga ggtgatagaa ccggttttgg tggaggtgat
    41221 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
    41281 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
    41341 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
    41401 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
    41461 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
    41521 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaataag
    41581 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
    41641 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
    41701 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
    41761 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
    41821 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
    41881 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
    41941 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
    42001 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
    42061 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
    42121 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
    42181 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
    42241 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
    42301 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
    42361 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
    42421 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
    42481 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
    42541 ccggtgacct gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
    42601 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
    42661 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
    42721 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
    42781 ccgtgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
    42841 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
    42901 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
    42961 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
    43021 ttgctttgaa ggaatcgagg atgcctttca gcttgccttc gatttcgtcg ttgtagccac
    43081 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
    43141 cggcttcgaa gctgccgatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
    43201 cgaacagaac cagagactgt tgcgcaacag acatcggcgc atactgtttc tgcttcagca
    43261 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
    43321 caaactgaga gaacgctgcc agttcacgat actgtgccag agcggtacgg ataccaccgg
    43381 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
    43441 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
    43501 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
    43561 taatcggcag cgcagtcaga gaaccggttt tccctttcac ttcacctttg gtgaaagctt
    43621 caacgtattc ggcgttaaca cgcgcagcac gctccagcag acgagagtgg aggtagaata
    43681 cgtcgcctgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
    43741 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
    43801 ggaagtattc acccattgcg caaccagcgt acggtgccag gtattgcagt gctgcggatt
    43861 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
    43921 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
    43981 cggaatcgcg ctggttgatg atggcatcga tagccagtgc ggttttaccg gtctgacggt
    44041 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
    44101 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
    44161 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
    44221 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
    44281 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
    44341 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
    44401 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
    44461 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
    44521 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
    44581 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
    44641 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
    44701 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
    44761 tgacgtctac ctcagcggta gcctcactca cggcacgcag gtgaataaac tgctccagaa
    44821 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
    44881 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
    44941 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
    45001 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
    45061 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
    45121 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
    45181 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
    45241 acgctcggct tcaatttccg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
    45301 ctcagctttc gcttcgtcca gaatctgcga gcggcgtttg ttcgcctgct cgatgattac
    45361 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
    45421 gtccttatgt gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
    45481 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
    45541 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
    45601 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
    45661 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
    45721 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaggaatt
    45781 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
    45841 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
    45901 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
    45961 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
    46021 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
    46081 atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac
    46141 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
    46201 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgct gtagaacaga
    46261 atcaggataa atacgcccag tgccatagac agcgttacgt tcacgtcagc agacggaacc
    46321 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcag gtcgataggc
    46381 agtaaatcca tcaggttcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    46441 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    46501 agctcaatcg cggtctgaaa cttacctggc acgccgctgg ttgccttttt ggctacacta
    46561 cggaataaaa ccaggaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    46621 attgtccaga aggtggctgg ggggttatgt ggatccacca gcgagaatgt acgcaggtcc
    46681 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    46741 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    46801 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    46861 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaggcca
    46921 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    46981 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    47041 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    47101 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    47161 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagcggtc
    47221 aaattatacg gtgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    47281 ttaaatattt ttccagtgca tacaattgcg acttttctgc aaaccctgtt cgatcatgaa
    47341 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    47401 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    47461 tcagtctaaa aacagatact gttttaataa atgacattta cataacaaaa accactcatt
    47521 gacattttta ataatgtttt aacagtcaat gatgattctt agcgccgatt tttagcagac
    47581 tgatattttc acttatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    47641 tacaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    47701 tgttaacagt ctaaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    47761 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    47821 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcat ttgccctttc
    47881 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    47941 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgacgg aaactcttct
    48001 accctgctct gtactggctc aatattctcc agtttaagct catgttgcac ctgacgaagg
    48061 aaacgcacgc gtttaccaag gctatccaac aacgtgaaat gagcttcagg acgcacgata
    48121 gagagtggaa tgccaggcag tcccggtccg gtgccgacat cgataaaccg ttcaccttgc
    48181 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    48241 cggaccgaag tcaggttgta cgctttattc catttatgca gcatattcac gtaggcaata
    48301 agctggtttt tctggtgatc ggtaagcgaa atacctgcgt ctttcagcag taaggagagt
    48361 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    48421 gcatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    48481 agaatggaga tagccgcagg cgtgacgcca gaaatacgcg aagcctggcc aatagaggcc
    48541 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    48601 tccagcgtcg ctggtagcag ggtattctcg ttacgcagct gcttttcgat ctcatcttgc
    48661 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc agcctgttca
    48721 tctgtcaacg caggggcaaa cggtgtcagc gtggttaatt tttcataagt catttcagga
    48781 cgacgtagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    48841 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    48901 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    48961 agttcgcgac cgatttcagt caaacgcaga tcagcattat cttcgcgcag catcagacga
    49021 tattctgcgc gcgaggtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    49081 tcaaccagta cgccgagata tgcctgagaa cgcgccggag cccaaccttc tttgtcagca
    49141 gacagacggg cagcgttaag accagccagc aaaccttgcg cagcggcttc ttcgtaaccg
    49201 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    49261 ggtttcaggt cgcgtggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    49321 ttcgcgtttt ccatcccctg catggagcgg acgatttgca tctgcacatc gaacggcagg
    49381 ctggtggaga taccgttcgg ataaatttcg ttagaggtca gcccttccgg ttcaaggaag
    49441 atctgatgct gatttctgtc ggcgaagcgc atgactttgt cttcgatcga cgggcagtag
    49501 cgtgggccga caccttcgat cacccctgcg tacattgggc tacgatcgag gttactgcgg
    49561 atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg ctggggatgc
    49621 tgggacgcat tgcccataaa cgagaatact ggcattgggt tatcgccatg ctgttgcgcc
    49681 agtacgctaa agtcgatggt tcgagcatca atacgcggtg gtgtcccggt tttcagacga
    49741 ccaacgcgca gcggcagttc acgcaaacgg cgagaaagcg gaatggacgg cggatcacca
    49801 gcacggccac cgctgtaatt atccagaccg atatgaattt taccgtcgag gaacgtccca
    49861 acggtgagca cgacagcttt ggcacggaac ttcagtccca tttgggtaac ggcaccgacc
    49921 acgcgatcgt tttcgacaat aagatcttca actgcctgct ggaagatcat caggttcggt
    49981 tggttctcca gcgccgtacg taccgcctga cggtagagca cacgatctgc ctgagctcgg
    50041 gtagcgcgaa ccgccggtcc cttgcttgcg tttagtatcc taaactggat acccgcctga
    50101 tcgatcgctt tcgccatcag accgccgagt gcatccactt cttttaccag atgtcccttc
    50161 ccaataccgc cgatcgccgg gttgcagctc atctgcccca gagtgtcgat attgtgtgtc
    50221 aaaagcagag tctgttgacc catacgcgcc gcggccatcg cggcctcggt gcctgcatga
    50281 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    50341 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    50401 gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga gatctgttct
    50461 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg gcttttaaga
    50521 tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac agtataagct
    50581 gggatcagga tgaggggtta tacacaactc aaaaactgaa caaccgttgt tctttggata
    50641 actaccggtt gatccaagct tcctgacaga gttatccaca gtggatcgca cgatctgtat
    50701 acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc ttccggaatg
    50761 tcgtgatcaa gaatgttgat cttcagtgtt tcgcccgtct gttttgcacc ggaatttttg
    50821 agttcagcct cgagtttatc aatagcccca caaaaggtgt catattcacg actaccaata
    50881 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    50941 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aaccagccag
    51001 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    51061 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    51121 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    51181 cgctgtgaaa gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    51241 gcttaatggt acgcatgatc gggttctgca ggacgatcag tgtctcggtg gactgaattt
    51301 catcaattgt ttggatcttg ttgataagta catgctggag agcatcgatc gaacggcaca
    51361 tcacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg acttcatcga
    51421 ggctttccag ctttgccagc gcggaaggat agtctttggc gctctttaat ataatgccga
    51481 taaagcagcc tacgtcataa ccaagctgct tcgggctgac atcaatacgc gccccggtaa
    51541 tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga ctgacgccaa
    51601 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    51661 cacggtccag attgtcgatt agataatttt ccataggatt ttcttatgcg gattgatgat
    51721 tcattctatt ttagccttct tttttaatga atcaaaagta cgtgaggctt tttattgaat
    51781 gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac gcaggagtat
    51841 aaaaaatgaa aaccgcttac attgccaaac aacgtcaaat tagcttcgtg aaatctcact
    51901 tttctcgtca actggaagaa cgtctggggc tgatcgaagt ccaggcgccg attcttagcc
    51961 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    52021 tgaaagctct gcctgatgcc cagtttgaag tggttcattc actggcgaag tggaaacgtc
    52081 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    52141 ttcgccccga tgaagaccgt ctttccccgc tgcactcggt ctatgtagac cagtgggact
    52201 gggaacgcgt aatgggcgac ggcgagcgtc aattctcgac tctgaaaagc acggtagagg
    52261 cgatctgggt gggaattaaa gcaaccgaag ctgcggttag cgaagagttt ggcctggcac
    52321 cgttcctgcc ggatcagatc catttcgtac acagccagga gttactgtct cgttatccgg
    52381 atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg
    52441 tcgggattgg cggcaagttg agtgatggtc atcgccacga cgtgcgcgca ccggattatg
    52501 atgactggag caccccgtca gagctgggcc atgcgggtct gaacggcgat attctggtat
    52561 ggaacccggt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    52621 acacgctgaa gcatcagctg gcgctgaccg gtgacgaaga tcgcctgcag ctggagtggc
    52681 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc gggcagtctc
    52741 gtttgaccat gctgctgctg caactgccac atatcggcca ggttcagtgt ggtgtatggc
    52801 cggcagctgt tcgtgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    52861 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    52921 gccgggtttg ccatgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    52981 caactctttc actttactcg tcacgtcgtc aggcaaccgc tgggcgataa aatcagaaat
    53041 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    53101 acaactggca agatcggtgc cgccacgaaa ctgctgactt aaaaagcgga tcgcttgctc
    53161 aataccttgt ggccctgaaa gctcataacg gacgatctca gtggaaaata gcataatata
    53221 gcagcgccgg ttttcagcga gggctatgcg catcaaggcc aggcagaacg ctttcgcaca
    53281 ctgttcatta aagccgccca ttgagccgga agtatccacg cagacaataa acggtccgcg
    53341 cggctgttcg tcgtaatctt tatgcaccac cgggcgttcg atcacttttt cacgccacga
    53401 ctcaccatgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    53461 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aagagacgta aaatatcatc
    53521 gctttgctgc agaccatcaa cctgctcagg aactgtcgcc ggttcgcgca ccatggtgcg
    53581 gaaggtttcc atctgcgcat cgttgcgcgg tattgatttg gcttcccggg aacgccccag
    53641 ctgttctgcc agacgtttca gttccggctg ttcgttaaga aattcaccgt atttcacaat
    53701 caactgatag tcgccacgtt taagctgacc ggcactcata tcccacagac gaccagctgc
    53761 ggtattgtta tctgcgagaa tcggttcaag ttgcccgctc agcgtcatgc gttcctgaac
    53821 ttcactcaac agttgttcgc gttcttcttc taatagctgc tgatttaacg tcgttgcttg
    53881 cacgatcagg cttaaacgcc agcgctggag aaaaagcgtg tgtaacgctg aagtgatcgt
    53941 actgttagca tcaaccaact gtcgggcttg ttctgcccat ggggaattca gacgatgcag
    54001 taagtccagg atttgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    54061 ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt tcagccgact
    54121 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gccgccttca gtcgtgggaa
    54181 tttttcaaag aagactgcca gctgcggtga ggccagcagc gcgatgatca tctcttcgat
    54241 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    54301 gcctggcgaa tctgtgcgcc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    54361 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt gcgccagtcg
    54421 ctttcaagct cttccagttg ttgttttatt tcgctgggca gaccttcagc cgatgaaccg
    54481 ggaagtgcta gcgtactgcc ttgtaaactc acatcgcgta caaccagatg ttgggcgcta
    54541 tcgacttcca gattcagttt ctgggcaaag ccgataccgt tcagtttgcc gcgaatctca
    54601 ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    54661 atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    54721 acagggagtt gatactgctg acgacggcta aaaatgccgc ccagacgaat taccgttaag
    54781 gctgttttat cgctttgttg ctgctgaagt tgcaggtgac gttgcacaat cgcgcccagg
    54841 cgggtcaaca tcccttgctg ttgccaggcg tgaccggtca tcaatacatc aatttgttgt
    54901 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatca
    54961 accggggcga cagcactgcg accgctaaaa aaggcgctgg cctgcaataa gcggatcgct
    55021 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    55081 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    55141 cagcgttcat attcttcatc tgtgatctgc aaggaggcag gaaccggatt gtcattttca
    55201 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cctgcacttt atctaaccac
    55261 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    55321 ttggaggccg ccaccagcag acgcatcggg attttttcta cgagtgcgcc gttgcggaac
    55381 tggcgctcgt taatggcggt gagcagggta ttaagaattg ccggacccgc tttccagatc
    55441 tcatccagaa agacgatttc agcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    55501 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    55561 cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    55621 atcaaacttt tggcaatacc tggtgggcca aggaggaaca cactttcacc acttaatgcc
    55681 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    55741 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    55801 ttcatcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    55861 tctcaaagga ggggtatttt tctttgagcc aggttaatgt ggccgcattt aggagtacga
    55921 ttttgccgtt aatcgtgcat actgtgcgct tttttgtggg ccaagggact aagcacacat
    55981 ttcatatttc aacgaaagac tagtctatga gcactgataa taagcaatca ttgcccgcga
    56041 ttaccctcgc ggcgattgga gttgtctacg gcgatattgg taccagcccg ttatatacac
    56101 ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga acgcgatgcc gtgtttggct
    56161 ttttatcgct gatcttctgg ctgctaatct ttgtggtttc cattaaatat ctcaccttcg
    56221 tgatgcgggc agataacgcc ggtgaagggg ggatcctgac gttgatgtcg cttgccgggc
    56281 gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat ggggctaatc ggcggcagct
    56341 ttttctatgg tgaagtcgtc attactcccg ctatttcggt gatgtccgcc attgaaggtc
    56401 tggaaatcgt cgccccgcag ctggatacct ggatagttcc cctctcaatt atcgttctca
    56461 cattattatt catgattcaa aaacatggca cggctatggt cggtaagctg tttgcgccga
    56521 tcatgctgac ctggtttttg attctggcag ggctggggtt acgtagcatt attgttaacc
    56581 cggaagtgct gcatgcactg aatccgatgt gggcggtgca tttcttcctt gaatacaaaa
    56641 cggtttcttt tattgcatta ggggcagtgg tgctgtcgat tacgggggtc gaggcgctgt
    56701 atgctgatat ggggcacttt ggtaagttcc ctattcgtct ggcgtggttc accgtcgtat
    56761 tgccttcctt aacccttaat tacttcggcc agggagcgct gttgttaaag aatccggaag
    56821 cgattaagaa cccgttcttc ctgttggcac cggactgggc gctgatcccg ctgctgatca
    56881 tcgccgcact ggcgacggta attgcctcgc aggcggttat ctctggcgtc ttctcattga
    56941 cgcgtcaggc ggtacgtctg ggatatttgt cgccgatgcg cattattcac acttccgaaa
    57001 tggagtcagg gcaaatctat attccctttg tgaactggat gctctatgtc gcggtcgtga
    57061 ttgtgattgt cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga
    57121 ccggaaccat ggtgctgacg tctattctct cgactaccgt ggcacgtcag aactggcact
    57181 ggaataagta ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attcccttgt
    57241 tcaccgctaa cctcgataaa ctgctctccg gcggctggtt gccattgagc ctcggtacgg
    57301 tgatgtttat cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc
    57361 atgaacatgg taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcccgttc
    57421 gcgtgcctgg gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga
    57481 tgcataacct gaaacataac aaggtattgc atgagcgggt gattctgtta actctgcgca
    57541 ccgaagacgc gccatatgtc cataacgtcc gtcgggtaca gattgaacaa ctgtcgccca
    57601 ctttctggcg cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt
    57661 tccaccgctg cggtctggaa ggattaagtt gccggatgat ggaaacctcc ttctttatgt
    57721 cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt
    57781 acttgctgct gcaacgtaat gcgctgcggg caccggatca atttgaaatc ccgccaaaca
    57841 gagttatcga actgggtact caggtcgaaa tctaacgcca gacgcctcct ttctccataa
    57901 gggggcgttt ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt
    57961 tccgtaacgt cacgatggtt ttcccaactc agtcaggatt aaactgcggg tcagcgaaac
    58021 gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg
    58081 gtgatctccc gactgggaca taccgatacg ctggtggtgt gtgatgctgg tttacccatc
    58141 cccaaaagta caacgcgtat cgatatggca ttaacccagg gtgtaccttc ttttatgcag
    58201 gtgctgggtg tagtcacaaa tgaaatgcag gtcgaggctg tcattatcgc ggaagagatc
    58261 aaacaacata atccgcaact ccacgaaacg ttgctcactc accttgagca gctgcaaaaa
    58321 caccagggaa acaccattga aattcgttac accacgcatg aacaattcaa acaacaaacc
    58381 gcagaaagtc aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc
    58441 tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga
    58501 taaagccttc ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct atccgggccg
    58561 cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac
    58621 tggtatctat actcgcgatg ccggtacgct tttatggttg gggaaagaaa cgacatttac
    58681 cgggccgaaa tcttcccagg aagccgggat tgggattatc caccaggaac tgaacctgat
    58741 cccgcagtta accattgccg aaaacatttt ccttggtcgt gagtttgtta atcgctttgg
    58801 caaaattgac tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct
    58861 gcgctttaaa agcgacaagc tggtgggcga tctttccatc ggtgaccagc aaatggttga
    58921 aatcgccaaa gtgctgagct ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc
    58981 gctgaccgat accgaaaccg aatccttgtt ccgcgtcatc cgcgagctga aatcgcaagg
    59041 ccgtggtatt gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt
    59101 taccgttttt cgtgacggac aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga
    59161 ttctctgatt gagatgatgg tgggccgcaa gctggaagat caatatccgc acctggacaa
    59221 agcgccggga gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt
    59281 ctcttttact ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgctgggcg
    59341 taccgaactg atgaaagtgc tctacggtgc actgccgcgc acaagcggtt acgtcaccct
    59401 ggatgggcat gaagtcgtta cccgttcgcc gcaggatggc ctggcaaacg gcattgttta
    59461 tatctccgaa gaccgtaaac gtgacggttt agtgctgggc atgtcagtaa aagagaacat
    59521 gtcgctgacc gcgctgcgct acttcagccg tgctggcggc agtttgaagc atgccgatga
    59581 acagcaggct gtgagtgatt tcattcgtct gtttaatgtg aaaacgccat cgatggaaca
    59641 ggcaattggt ctgctttccg gtggcaatca gcaaaaagta gcgattgccc gtggtctgat
    59701 gacacgccct aaagtgttga tcctcgatga gcctacccgt ggcgtagatg tcggcgcgaa
    59761 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt
    59821 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
    59881 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
    59941 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
    60001 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
    60061 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
    60121 atattcttca gcaaacctcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
    60181 tgacgtcggg tatcgacttg tcggtaggtt ctctgttggc gctgaccggc gcagttgctg
    60241 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag
    60301 gtgccgcaat tggtgcggta actggggtga ttgtagcgaa aggtcgcgtc caggcgttta
    60361 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaatggta
    60421 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
    60481 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
    60541 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
    60601 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
    60661 gtctgctggc atcgctggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca
    60721 ctgcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
    60781 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
    60841 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
    60901 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
    60961 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
    61021 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
    61081 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataaa cttggctata
    61141 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
    61201 taaccgttcg cggtaccaaa attctgctga ttaacccgac cgactccgac gcagtaggta
    61261 atgctgtaaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
    61321 cgaaaggtga agtggtgagc cacattgcat ctgataacgt actgggcggc aaaatcgccg
    61381 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
    61441 ccggtacatc cgcagcccgt gaacgtggcg aaggtttcca gcaggccgtt gctgctcaca
    61501 agtttaatgt tcttgccagc cagcctgcag attttgatcg caccaaaggt ttgaacgtaa
    61561 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
    61621 tggcgctggg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
    61681 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
    61741 tcgctcagct acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga
    61801 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
    61861 tttaatcagg ttacatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
    61921 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
    61981 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
    62041 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
    62101 atttggcggc aaaggcgcga atcaggcggt ggctgctggg cgtagcggtg cgaatatcgc
    62161 gtttattgcc tgcactggcg atgacagcat tggtgagagc gttcgccagc agctcgccac
    62221 tgataacatc gatatttctc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
    62281 gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc
    62341 cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cggcattatt
    62401 aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
    62461 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aacttctggc
    62521 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
    62581 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac
    62641 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
    62701 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
    62761 cgcgttaatc acggcgttgc tggaagaaaa aacattgcca gaggcgattc gttttgccca
    62821 tgcagccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
    62881 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
    62941 ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
    63001 cagtgaagcg attacagcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
    63061 agctctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
    63121 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
    63181 cggttatagt cttgtccttt gcaataccga aggcgatgaa cagcggatga atcgtaatct
    63241 ggaaacgctg atgcaaaaac gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca
    63301 gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct accgtgatga tggactgggc
    63361 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
    63421 aacgcaatat ctgatcgata aaggtcatac ccgtattgcc tgtattaccg gtccgctgga
    63481 taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct
    63541 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
    63601 tatgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
    63661 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
    63721 cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaacaac
    63781 tatacaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
    63841 aacccagccg accctacagc aacaacgatt acaacttact ccgattctga tggaacgtgg
    63901 ttcggcttag atttacgctg tcttttgatc aaattattac catcggttgt tttcagtagc
    63961 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
    64021 tgttcgactg tcgttgtgcc ttccatccct tcgtaaacgc gaaggactgc cgcacttaca
    64081 gcaacgccta aactgatcga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
    64141 gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ttgtgtcgac
    64201 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgctggtgat
    64261 tgcaaagaga actgagcgat cattagccct ataatcaccg tgatccccac taacgtatgg
    64321 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
    64381 gctgtcggtg ccatcataca gccagcaata aacgcctgat acccaaatcc tacctgtaac
    64441 atcaatggca taaggaacgg tacacagccg gtccccagac gggttgcaat attgcctacg
    64501 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg
    64561 cgtcgcgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat taccgtcaag
    64621 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca
    64681 aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa
    64741 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa
    64801 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
    64861 ggccccacca gacccggcat ggcgacaaaa ttcaatacag gaagaagttc attacgagga
    64921 taagcgcgca gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga
    64981 ataacccgga agacaaccag ctgtggtagc gagttggaaa gtgcgcaggc cagagaaccc
    65041 aacgtgaaca gactcacggc aagggtaaaa atgcgacgtg taccgaagcg atcggctagc
    65101 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
    65161 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
    65221 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata
    65281 ctgcgcttct ttttatcgct cattcaatgt cctgttatcg ggttatcact tatctggtga
    65341 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc
    65401 gacaatcgct tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
    65461 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
    65521 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
    65581 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt
    65641 gccaaccttt gctgccagta ggcaagcttg cggctccaga cagatacgca taaccagaaa
    65701 gtgatcgatg acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc
    65761 aagaaaattc cagttcgatt gtggcatgac tcgggtacca attcgcggtc gcggtaaaac
    65821 catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa
    65881 ttgctcgccc agctcaatct caccaggcaa aatggtgccg ggttcatatt caccttttaa
    65941 gatccgctgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
    66001 ttgtgcgctt aaaggcatta ctaatcttcc tttttctttt tattccttct tagtatgcca
    66061 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg
    66121 gggcttttgc tgaaaaaatg cgcggtcgga aaattatttt aaatttcctc ttgtcaggcc
    66181 ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca cgccgccggg
    66241 tcagcggggt tctcctgaga atctcaacag agaaaagcaa aaaaatgctt gactctgtag
    66301 cgggaaagcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg
//
LOCUS       DAZFRE010000015        65709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFRE010000015 DAZFRE010000000
VERSION     DAZFRE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.015"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..455)
                     /gene="folK"
                     /locus_tag="ACHKUI_001169"
     CDS             complement(<1..455)
                     /gene="folK"
                     /locus_tag="ACHKUI_001169"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6597410.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKG"
     gene            complement(452..1849)
                     /gene="pcnB"
                     /locus_tag="ACHKUI_001170"
     CDS             complement(452..1849)
                     /gene="pcnB"
                     /locus_tag="ACHKUI_001170"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6597411.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFTLAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1909..2805)
                     /gene="gluQRS"
                     /locus_tag="ACHKUI_001171"
     CDS             complement(1909..2805)
                     /gene="gluQRS"
                     /locus_tag="ACHKUI_001171"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6597412.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="ACHKUI_001172"
     CDS             complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="ACHKUI_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6597413.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="ACHKUI_001173"
     CDS             complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="ACHKUI_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6597414.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="ACHKUI_001174"
     CDS             complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="ACHKUI_001174"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6597415.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIRWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4828..7257
                     /gene="hrpB"
                     /locus_tag="ACHKUI_001175"
     CDS             4828..7257
                     /gene="hrpB"
                     /locus_tag="ACHKUI_001175"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6597416.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCGDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLARAYADRIARRRGQDE
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7351..9879
                     /gene="mrcB"
                     /locus_tag="ACHKUI_001176"
     CDS             7351..9879
                     /gene="mrcB"
                     /locus_tag="ACHKUI_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6597417.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQLLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10099..12357
                     /gene="fhuA"
                     /locus_tag="ACHKUI_001177"
     CDS             10099..12357
                     /gene="fhuA"
                     /locus_tag="ACHKUI_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6597418.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12408..13205
                     /gene="fhuC"
                     /locus_tag="ACHKUI_001178"
     CDS             12408..13205
                     /gene="fhuC"
                     /locus_tag="ACHKUI_001178"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6597419.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13205..14095
                     /gene="fhuD"
                     /locus_tag="ACHKUI_001179"
     CDS             13205..14095
                     /gene="fhuD"
                     /locus_tag="ACHKUI_001179"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6597420.1"
                     /translation="MSGLPLISRRRLLTAMALSPFLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLTHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPYVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14092..16074
                     /gene="fhuB"
                     /locus_tag="ACHKUI_001180"
     CDS             14092..16074
                     /gene="fhuB"
                     /locus_tag="ACHKUI_001180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6597421.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGTRRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16109..17389)
                     /gene="hemL"
                     /locus_tag="ACHKUI_001181"
     CDS             complement(16109..17389)
                     /gene="hemL"
                     /locus_tag="ACHKUI_001181"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6597422.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17555..17602
                     /gene="yadX"
                     /locus_tag="ACHKUI_001182"
     CDS             17555..17602
                     /gene="yadX"
                     /locus_tag="ACHKUI_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6597423.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17614..19035
                     /gene="clcA"
                     /locus_tag="ACHKUI_001183"
     CDS             17614..19035
                     /gene="clcA"
                     /locus_tag="ACHKUI_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6597424.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHCVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19059..19124
                     /gene="yadW"
                     /locus_tag="ACHKUI_001184"
     CDS             19059..19124
                     /gene="yadW"
                     /locus_tag="ACHKUI_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6597425.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19117..19461
                     /gene="erpA"
                     /locus_tag="ACHKUI_001185"
     CDS             19117..19461
                     /gene="erpA"
                     /locus_tag="ACHKUI_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6597426.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19508..20131)
                     /gene="yadS"
                     /locus_tag="ACHKUI_001186"
     CDS             complement(19508..20131)
                     /gene="yadS"
                     /locus_tag="ACHKUI_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6597427.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20169..20969)
                     /gene="btuF"
                     /locus_tag="ACHKUI_001187"
     CDS             complement(20169..20969)
                     /gene="btuF"
                     /locus_tag="ACHKUI_001187"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6597428.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIGQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20962..21660)
                     /gene="mtnN"
                     /locus_tag="ACHKUI_001188"
     CDS             complement(20962..21660)
                     /gene="mtnN"
                     /locus_tag="ACHKUI_001188"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6597429.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21744..23261
                     /gene="dgt"
                     /locus_tag="ACHKUI_001189"
     CDS             21744..23261
                     /gene="dgt"
                     /locus_tag="ACHKUI_001189"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6597430.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23391..24815
                     /gene="degP"
                     /locus_tag="ACHKUI_001190"
     CDS             23391..24815
                     /gene="degP"
                     /locus_tag="ACHKUI_001190"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6597431.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24970..26127
                     /gene="cdaR"
                     /locus_tag="ACHKUI_001191"
     CDS             24970..26127
                     /gene="cdaR"
                     /locus_tag="ACHKUI_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO6597432.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26181..26567)
                     /gene="yaeH"
                     /locus_tag="ACHKUI_001192"
     CDS             complement(26181..26567)
                     /gene="yaeH"
                     /locus_tag="ACHKUI_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6597433.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26729..27541)
                     /gene="yaeI"
                     /locus_tag="ACHKUI_001193"
     CDS             complement(26729..27541)
                     /gene="yaeI"
                     /locus_tag="ACHKUI_001193"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO6597434.1"
                     /translation="MISRRRFLQATAATIATGSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASKANLPRLVLAHNPDSKEVMRDDPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27595..28419)
                     /gene="dapD"
                     /locus_tag="ACHKUI_001194"
     CDS             complement(27595..28419)
                     /gene="dapD"
                     /locus_tag="ACHKUI_001194"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6597435.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28450..31122)
                     /gene="glnD"
                     /locus_tag="ACHKUI_001195"
     CDS             complement(28450..31122)
                     /gene="glnD"
                     /locus_tag="ACHKUI_001195"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6597436.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31184..31978)
                     /gene="map"
                     /locus_tag="ACHKUI_001196"
     CDS             complement(31184..31978)
                     /gene="map"
                     /locus_tag="ACHKUI_001196"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6597437.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32346..33071
                     /gene="rpsB"
                     /locus_tag="ACHKUI_001197"
     CDS             32346..33071
                     /gene="rpsB"
                     /locus_tag="ACHKUI_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6597438.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33206..34057
                     /gene="tsf"
                     /locus_tag="ACHKUI_001198"
     CDS             33206..34057
                     /gene="tsf"
                     /locus_tag="ACHKUI_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6597439.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34204..34929
                     /gene="pyrH"
                     /locus_tag="ACHKUI_001199"
     CDS             34204..34929
                     /gene="pyrH"
                     /locus_tag="ACHKUI_001199"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6597440.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35079..35636
                     /gene="frr"
                     /locus_tag="ACHKUI_001200"
     CDS             35079..35636
                     /gene="frr"
                     /locus_tag="ACHKUI_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6597441.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35728..36924
                     /gene="ispC"
                     /locus_tag="ACHKUI_001201"
     CDS             35728..36924
                     /gene="ispC"
                     /locus_tag="ACHKUI_001201"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6597442.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVARMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37113..37871
                     /gene="ispU"
                     /locus_tag="ACHKUI_001202"
                     /gene_synonym="uppS"
     CDS             37113..37871
                     /gene="ispU"
                     /locus_tag="ACHKUI_001202"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6597443.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37884..38741
                     /gene="cdsA"
                     /locus_tag="ACHKUI_001203"
     CDS             37884..38741
                     /gene="cdsA"
                     /locus_tag="ACHKUI_001203"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6597444.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38753..40105
                     /gene="rseP"
                     /locus_tag="ACHKUI_001204"
     CDS             38753..40105
                     /gene="rseP"
                     /locus_tag="ACHKUI_001204"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6597445.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40135..42567
                     /gene="bamA"
                     /locus_tag="ACHKUI_001205"
     CDS             40135..42567
                     /gene="bamA"
                     /locus_tag="ACHKUI_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6597446.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42689..43174
                     /gene="skp"
                     /locus_tag="ACHKUI_001206"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42689..43174
                     /gene="skp"
                     /locus_tag="ACHKUI_001206"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6597447.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43178..44203
                     /gene="lpxD"
                     /locus_tag="ACHKUI_001207"
     CDS             43178..44203
                     /gene="lpxD"
                     /locus_tag="ACHKUI_001207"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6597448.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDCVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44308..44763
                     /gene="fabZ"
                     /locus_tag="ACHKUI_001208"
     CDS             44308..44763
                     /gene="fabZ"
                     /locus_tag="ACHKUI_001208"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6597449.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44767..45555
                     /gene="lpxA"
                     /locus_tag="ACHKUI_001209"
     CDS             44767..45555
                     /gene="lpxA"
                     /locus_tag="ACHKUI_001209"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6597450.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45555..46703
                     /gene="lpxB"
                     /locus_tag="ACHKUI_001210"
     CDS             45555..46703
                     /gene="lpxB"
                     /locus_tag="ACHKUI_001210"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6597451.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46700..47296
                     /gene="rnhB"
                     /locus_tag="ACHKUI_001211"
     CDS             46700..47296
                     /gene="rnhB"
                     /locus_tag="ACHKUI_001211"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6597452.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47333..50815
                     /gene="dnaE"
                     /locus_tag="ACHKUI_001212"
     CDS             47333..50815
                     /gene="dnaE"
                     /locus_tag="ACHKUI_001212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6597453.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGALVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50828..51787
                     /gene="accA"
                     /locus_tag="ACHKUI_001213"
     CDS             50828..51787
                     /gene="accA"
                     /locus_tag="ACHKUI_001213"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6597454.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51885..54026
                     /gene="ldcC"
                     /locus_tag="ACHKUI_001214"
     CDS             51885..54026
                     /gene="ldcC"
                     /locus_tag="ACHKUI_001214"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6597455.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54083..54472
                     /gene="yaeR"
                     /locus_tag="ACHKUI_001215"
     CDS             54083..54472
                     /gene="yaeR"
                     /locus_tag="ACHKUI_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6597456.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54537..55850
                     /gene="tilS"
                     /locus_tag="ACHKUI_001216"
     CDS             54537..55850
                     /gene="tilS"
                     /locus_tag="ACHKUI_001216"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6597457.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55884..56144)
                     /gene="rof"
                     /locus_tag="ACHKUI_001217"
     CDS             complement(55884..56144)
                     /gene="rof"
                     /locus_tag="ACHKUI_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6597458.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLALELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56131..56331)
                     /gene="yaeP"
                     /locus_tag="ACHKUI_001218"
     CDS             complement(56131..56331)
                     /gene="yaeP"
                     /locus_tag="ACHKUI_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6597459.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56497..57042
                     /gene="yaeQ"
                     /locus_tag="ACHKUI_001219"
     CDS             56497..57042
                     /gene="yaeQ"
                     /locus_tag="ACHKUI_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6597460.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57039..57461
                     /gene="arfB"
                     /locus_tag="ACHKUI_001220"
     CDS             57039..57461
                     /gene="arfB"
                     /locus_tag="ACHKUI_001220"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6597461.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57475..58185
                     /gene="nlpE"
                     /locus_tag="ACHKUI_001221"
                     /gene_synonym="cutF"
     CDS             57475..58185
                     /gene="nlpE"
                     /locus_tag="ACHKUI_001221"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6597462.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(58341..59165)
                     /gene="yaeF"
                     /locus_tag="ACHKUI_001222"
     CDS             complement(58341..59165)
                     /gene="yaeF"
                     /locus_tag="ACHKUI_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO6597463.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59218..60936)
                     /gene="proS"
                     /locus_tag="ACHKUI_001223"
     CDS             complement(59218..60936)
                     /gene="proS"
                     /locus_tag="ACHKUI_001223"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6597464.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(61047..61754)
                     /gene="tsaA"
                     /locus_tag="ACHKUI_001224"
     CDS             complement(61047..61754)
                     /gene="tsaA"
                     /locus_tag="ACHKUI_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6597465.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61751..62155)
                     /gene="rcsF"
                     /locus_tag="ACHKUI_001225"
     CDS             complement(61751..62155)
                     /gene="rcsF"
                     /locus_tag="ACHKUI_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6597466.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62273..63088)
                     /gene="metQ"
                     /locus_tag="ACHKUI_001226"
     CDS             complement(62273..63088)
                     /gene="metQ"
                     /locus_tag="ACHKUI_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6597467.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63128..63781)
                     /gene="metI"
                     /locus_tag="ACHKUI_001227"
     CDS             complement(63128..63781)
                     /gene="metI"
                     /locus_tag="ACHKUI_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6597468.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63774..64805)
                     /gene="metN"
                     /locus_tag="ACHKUI_001228"
     CDS             complement(63774..64805)
                     /gene="metN"
                     /locus_tag="ACHKUI_001228"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6597469.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64993..65565
                     /gene="gmhB"
                     /locus_tag="ACHKUI_001229"
     CDS             64993..65565
                     /gene="gmhB"
                     /locus_tag="ACHKUI_001229"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6597470.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15475 a        16344 c        18104 g        15786 t
ORIGIN      
        1 ccttttgcct gtagagcttc aactaatgat aagccatcag gaaataccaa ttccggcgca
       61 atttcaaaca gtggccacag cataaagcca cgatttttca tatcgtagtg cggtacggtc
      121 agacgttcgg tatttattac ttcattacca aacagcatga tgtcgagatc cagcgtgcgc
      181 ggtccccagc gttcagcttt acggacgcga ccttgctgca attcgatacg ttgtgtgtga
      241 ttgagtagct cttcaggggc aagagtggtt tccagcgcga cggcggcatt taagtaatcg
      301 ggttgatctt gcggccccag cggtggggtg cggtaaaacg aagaaacagc aagaatgcgg
      361 ctttctggaa tatcgcctaa tgctttcagg gcagcattga cctgctccag cggagaggcc
      421 agattgctgc ctatggcaat atacgccact gtcatgcggt accctcacga cgcggtgcgc
      481 gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc ttcatccagt tcgttaagca
      541 tccctttttg gtctggtggc gcggaaacct ggaactcacc ccaccatttc accagacgtt
      601 gcagttcagc gttacgctca acttcggctc gtaaggccaa caggtcgtat gccgcacgga
      661 acttaggatg ctccagcagt ttccatgcgc gttttccctg acgacgggac atacgcaact
      721 gcaactgcca gatatcgcgg gttagcgtgg tcagccgttt cgggatcgcc agtgaacggc
      781 aggcttcgtc cagcacgtcg ttcatcgcca gtgtgaaagc gtcgtgatag gtcaggccgc
      841 tttcctgggc gatcttctgt gccgtctcca gcaatgggta ccagaacatg gcggcaaaca
      901 ggaacgccgg gttcacgcgc atatcgttat ggatacgcgt atcggtattc ttcagtacct
      961 gctcaataat gcgttccatc gggctgtcgc cattttccgt gaagtagcgg gtaatggtcg
     1021 ggaacagcgg ctggaacaga tgatattcac acagcagctt ataggtttcg taaccgtagc
     1081 ccgcttgcaa cagtttcagc gattcttcaa acagacgtgc cggtgggata tcgttcagta
     1141 aggttgccag gcgtgggatc ggctcggcgg tttccgggct gatgcgcatg cccaatttgg
     1201 cggcaaaacg aaccgcgcga agcatccgta ccggatcttc acggtagcgc gtttccgggt
     1261 taccaatcag acggataacg ccgtctttca gatccttcat gccgccaacg taatcacgga
     1321 cggtaaaatc tgccacgctg taatacaggc tgttgatagt gaaatcgcgg cgctgggcgt
     1381 cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat accgttttgc ccgcgttggg
     1441 aggtcgtgcg gtcgctgacg ttaccttcgt gatgtccacg gaaggtcgca acttcgataa
     1501 tctccgggcc aaacatcaca tgagccagac ggaaacggcg acccaccagg cggcagttac
     1561 ggaacagttt gcgcacctgc tctggcgtgg cgttggtggt cacgtcaaaa tctttcggct
     1621 ttttgccaag taacaggtcg cgcacgccgc cgccaaccag ccaggcttcg tatcccgctt
     1681 tattgagcct gtacattacc ttcagggcat tttcactgat atctttgcgg gaaatagcat
     1741 gctgctcacg cgggatcacc gtcacctgtg gacgggcgac tgcctgttca gcctcgcttt
     1801 cctcgcggct tagcaccttg cggcaaaaat tagcgactcg ggtaaaaata gtacacctcg
     1861 gtagtgtcaa acatcattca ggacaaaaaa atagcggcta atcatagctc agcatgacgc
     1921 atttgagaat gttgaattta caattgccga ctcaggcacg gcggtcagcg tccagttttt
     1981 gacggctgac tgaaggattt gctcgacgct gaaatcctgc cagtgtgttt ctacctgctg
     2041 acccagaaaa tgaagtgccg cgattaacac cgggcgcgga tcgcctttcg gcaacgcagg
     2101 cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta agcgccagcg gtaaatggat
     2161 gtaatctggc actttccagc caaaaagctg gtacagcgag atttgccgca ccgtcggttc
     2221 gatcaaatct gccccgcgga caatttcgct tacgccctgg aagtgatcat ccaccaccac
     2281 cgccaggtta taggcaaaca aaccatcacg gcgatgaata ataaaatctt cccgtgccag
     2341 tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta aactgggtga ccggatgctg
     2401 ctggcggata cgtactgcgg cgttgtctgg tccatgatgc aaatcccggc aatgaccgtc
     2461 gtaaataccg ccaatgcttt gaatacgcgc acgcgtgcag gtgcagtaat aacttagtcc
     2521 ttgctcatgt aaccaggcga gtgcttcgcg ataggcgtgg tgacgttgcg attgccagag
     2581 aacgtcgccg tcccagtaca ggccgtaatg ttccagctgg cgcaggatag tttctgcggc
     2641 accgggaact tcacgaggcg ggtcgatatc ttcaatgcgt accagccagc gaccttgccg
     2701 ggcgcgagcc tgcaaatagc tgccaagcgc agcaatcaga gaaccaaaat gaagctcgcc
     2761 ggaaggagag ggggcgaagc ggccaatata gtgtgtgtct gtcatctctt tgaacaaaaa
     2821 ataaggcggg agcatttccc gcctgtggta aacgtgatgg aacggctgta attagccagc
     2881 catctgtttt tcgcgaattt cagccagcgt tttgcagtcg atgcacagat cggctgtcgg
     2941 gcgcgcttcc agacggcgaa taccgatttc aacaccgcag gattcgcagt agccgaaatc
     3001 ttcgtcttcc acttttttca gcgtcttctc gatctttttg atcagcttac gctcgcgatc
     3061 gcggttacgc agttcgaggc tgaactcttc ttcctgggct gcacggtcta ccgggtccgg
     3121 gaagttggct gcttcatcct gcatatgtgt aacggtgcga tcgacttcat ccctgagttg
     3181 attacgccat gcttccagaa tacgacggaa gtgcgccagc tgggcttcat tcatatactc
     3241 ttcgcccggc ttctcctgat atggttccac cccagcgatg gcgagaatac tcagggacga
     3301 tgttttacgg ttttgccctt cttgcatgtt gcttctcctt aacacgcact atcgatcccc
     3361 atgttcgggg gaaaaatgag gccgctataa atagcagatg cttttccgga tagcaattat
     3421 ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat ttgcgcacgc gaccagaatg
     3481 taaattaacc agttacttac tctactacaa tgtaaccggt agtgattttt taagagccat
     3541 gccttcggca gaaagttccg ctttgtaagc cagaatttct acccccctct gttgagcttc
     3601 tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg ggtgaaaacc gtgtaatggc
     3661 tgaatgcagc acggcgaaaa agataaccgc acgctggcct tcagccgcta cgctcatcaa
     3721 ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg ggaaaatatc cctgttcgtt
     3781 ctccgctaac gtaaccgatt tcacttcaat atagcagtct ggacgcgaat ccgcctgcaa
     3841 cataaagtca atacggctgc gttcggcgcc gtattttact tcgcttttca gcgagctata
     3901 gcctgccagc tctgaaattg attcattaag gatagcctct ttcgtcaacc tgttagccca
     3961 aagcgtattg acgcaaataa ttgcgccgct ctggctttga gttaattccc aggtgtgtgg
     4021 gtatttccgt ttggtgttgt ctgaagtcga ataccagacg gtatcgccag gcgttgcaca
     4081 accggtcatc gcacccgtat tcgggcagtg tagcgttaat tcgcgaccat cgggtgtgat
     4141 cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc gcgcgctgta gagggggaga
     4201 aaattccatt gcgacaatcc ttgttattgc gttagcgccc agcgttttag cggcgtgtag
     4261 cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga actccgtcac cgtatacgac
     4321 cagttaaaac ctggcggcgg gattgtcacc gcctcgctgg cgtcgcgcaa taaggtaata
     4381 tgtggatgaa acggacgatt gctttgaaaa caaccgctgc gggcagcctg tgagcggagc
     4441 atattcgcca gctggattaa gccgcgaggc ggctgtcgca tccctaacca caccacacgc
     4501 gaacgcagcc attgaccggc gtcatcaagc gtgagtgtga aaccaggttg acgaatccgt
     4561 ccggctaaaa gagaaagcgc cttctctttc tctgcgctca cttcgcctaa aaatgccaga
     4621 gtcagatgca aattatcggc ggcgaccgga cgtcccgcct caggtgggaa gtgtgtggcg
     4681 cgccagcgga taatctgttc gcggatttct gcaggtaagt cgatagcaaa gaacagccgt
     4741 tgcggttcag acatgtgagg cactcggtta tgaattaccg cgatgctaca atgtggcgcg
     4801 aagaatgtta accctctgga gcgttttgtg tcgtcgttgc ccgttgctgc cgtcttacct
     4861 gagttacttg ctgccctcga tggtgcatcg caggtgttat taagtgcgcc gaccggggcc
     4921 gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc ccggcattaa cgggaaaatt
     4981 atcctgctgg agccgcgtcg tctggcggcg cgtaacgttg cgcaacgact ggcggagctg
     5041 cttaacgaaa agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg
     5101 ccgaataccc gcctggaagt ggttaccgaa ggcgtgctga cgcggatgat ccagcgtgac
     5161 ccggaactga gtggtgttgg attggtgatc cttgatgaat ttcatgagcg cagcttgcag
     5221 gcggatttgg cattggcgtt gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa
     5281 ctgctgatta tgtcggctac gctggacaac gaccgcttgc agcaaatgct gccagaagcg
     5341 cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac gccgttattt gccgctgccc
     5401 gctcatcagc gttttgacga agccgtcgcg gttgctaccg ccgaaatgct gcgtcaggaa
     5461 agtggatcat tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggaacaa
     5521 ctgacttcac gcatcggcag tgatgtgttg ctctgcccgc tgtatggcgc gttgtcgctg
     5581 aacgatcagc gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg
     5641 accaatattg ctgaaacaag tttaaccatc gaaggtattc gtctggtggt ggattgtgcc
     5701 caggaaaggg tggcgcgttt tgatccgcgt acagggctta cgcggttgat tactcaacgc
     5761 gttagtcagg cgtcgatgac gcaacgtgcc gggcgtgccg ggcgtctgga gccgggtatc
     5821 tgcctgcatt taatcgccaa agaacaagca gaacgcgctg ccgcgcaaag tgaaccggag
     5881 atcttacaaa gcgatctttc cggtttgctg atggaattac tgcaatgggg atgcggcgat
     5941 ccggcgcaga tgagctggct ggatcaaccg ccaacggtga atctactggc cgcgaaacgc
     6001 ctgttacaga tgctgggggc gctggatggc gaacgcctaa gcgcgcaagg gcaaaaaatg
     6061 gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac
     6121 gaagctgcta ccgcggcaaa aattgccgcc attctcgaag agccgccacg gatgggcaat
     6181 agtgacctgg gcgtggcgtt ttcgcgcaat caacccgcct ggcagcaacg tagtcagcaa
     6241 ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca gttcgcttat cgcgccgcta
     6301 cttgccagag cgtatgccga tcgcattgct cgtcgccgtg ggcaagatga acgctatcaa
     6361 ctggcgaacg gcatgggggc gatgctcgat gccgacgatg cgctaagccg ccacgaatgg
     6421 ttgatcgcac cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg
     6481 ctgccggtcg atattgatga gttagtacaa cgctgcccgc agctggtaca gcagtctgac
     6541 actgtggagt gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct gcaaattggt
     6601 cagttgatgg taaaagtgca accgctggcg aaaccctcgg aagacgagtt gcatcaggcg
     6661 atgcttaacg gcattcgtga taaaggttta agcgtgctca actggacggc ggaagcggaa
     6721 cagctacgct tgcgtttgtt atgcgccgca aagtggttgc cggaatatga ctggccagcg
     6781 gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc tactgccgca tatgacaggc
     6841 gtacattcgc tacgcggcct gaaatcactc gatatttatc aggcactgcg tggattactt
     6901 gattgggtaa tgcagcaacg tctggatagt gaattgcctg cgcattacac tgtgccgacg
     6961 ggaagccgga tcgccattcg ttaccatgaa gataacccgc ccgcgctggc ggtgagaatg
     7021 caggagatgt ttggtgaggc cactaatccg actatcgccc aggggcgcgt accgttggtg
     7081 ctggagttgc tttcccctgc ccaaaggccg ctgcaaatca cgcgtgattt gagcgccttc
     7141 tggaaaggag cgtaccgtga ggtgcaaaaa gagatgaaag ggcgttatcc caaacatgtc
     7201 tggccggacg acccggcaaa caccgcaccg acgcgacgaa cgaaaaagta ttcgtgaaga
     7261 ccaaagcaaa tttgagagat atcttcttct gtcttgcaac agaagaacag aaaatcgggc
     7321 ttttgcgcct gaatattgcg gagaaaaagc atggccggga atgaccgcga gccaattgga
     7381 cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa gccgtcgtcg ttacgaagat
     7441 gacgatgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc gcgcaaaggt
     7501 aagggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc tatggctgct gctaaaactg
     7561 gctatcgttt ttgccgtgat gatcgccatt tacggcgttt atctcgatca aaaaattcgc
     7621 agccgtattg atggcaaggt ctggcaattg cccgcggcag tttatggtcg aatggtcaac
     7681 cttgagccag acatgaccat cagcaagaac gagatggtga agctgctgga ggcgacccag
     7741 tatcgccagg tctcgaaaat gacccgtcct ggcgagttta ccgtacaggc caacagcatt
     7801 gagatgatcc gccgtccgtt tgatttcccg gacagcaaag aagggcaggt gcgcgcgcgt
     7861 ctgacctttg atggcgatca tctggcgacg atcgtcaata tggagaacaa ccgtcagttc
     7921 ggtttcttcc gtcttgatcc gcgcctgatc accatgattt cttcgccaaa cggtgagcag
     7981 cgtctgtttg tgccgcgcag tggtttcccg gatttgctgg tggatacttt gttggcgaca
     8041 gaagaccgtc atttctacga gcatgatggg atcagtctct actcaatcgg acgtgcggtg
     8101 ctggcaaacc tgactgccgg acgcacggtg cagggggcga gtacgctgac gcaacagttg
     8161 gttaaaaacc tgttcctctc cagcgagcgt tcttactggc gtaaagcgaa cgaagcctac
     8221 atggcgctga tcatggacgc gcgttacagc aaagaccgta ttcttgagct gtatatgaac
     8281 gaggtgtatc tcggtcagag cggtgacaac gaaatccgcg gcttcccgct ggcgagcttg
     8341 tattactttg gtcgcccggt agaagagcta agcctcgatc agcaggcgct gttagtcggt
     8401 atggtgaaag gggcgtccat ctacaacccg tggcgtaacc cgaaactggc gctggagcga
     8461 cgtaatctgg tactacgttt gctgcaacag caacaaatta ttgatcaaga actctacgac
     8521 atgctgagtg cgcgtccgct gggtgttcag ccgcgcggtg gggtgatctc ccctcagcca
     8581 gcctttatgc agctggtgcg tcaggagctg caggcgaaat taggcgataa ggtaaaagat
     8641 ctctccggcg tgaagatctt cactaccttt gactcggtgg cccaggacgc ggcagaaaaa
     8701 gccgctgtgg aaggcattcc ggcactgaag aaacagcgta agttgagcga tcttgaaacc
     8761 gcgattgtgg ttgttgaccg ctttagcggt gaagttcgtg cgatggtcgg tggctctgag
     8821 ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca
     8881 aaaccagcga cttatctaac ggccttaagc cagccgaaaa tctatcgttt gaatacgtgg
     8941 attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc aggtctggtc accgcagaac
     9001 gatgatcgtc gttatagcga aagtggcagg gtgatgctgg tagatgcgtt gacccgttcg
     9061 atgaacgtgc cgacggtgaa tctgggcatg gcgttggggc tgcctgcggt tacggagacc
     9121 tggattaaac tgggtgtgcc gaaagatcaa ctgaatccag ttccagcaat gttgttgggg
     9181 gcgttgaact taacgccaat cgaagtggcg caggccttcc agactatcgc cagcggtggt
     9241 aatcgtgctc cgctttccgc gctacgttca gtcatcgcag aagacggcaa ggtgttgtat
     9301 cagagcttcc cgcaggcgga acgcgctgtt ccggcgcagg cggcgtatct gacactatgg
     9361 accatgcagc aggttgtaca acgcggtacg ggacgtcagc ttggggcgaa atacccgaac
     9421 ctgcatctgg cagggaaaac agggactacc aacaataacg tagatacctg gtttgcgggc
     9481 attgatggga gcacggtgac catcacctgg gtcggccgtg ataacaacca gccgaccaaa
     9541 ctgtatggtg ccagcggggc aatgtcgatt tatcagcgtt atctggctaa ccagacgcca
     9601 acgccgctga atcttgttcc gccagaagat attgcagata tgggcgtgga ctacgacggc
     9661 aactttgttt gcagcggtgg catgcgtgtc ttgccggtct ggaccagcga tccgcaattg
     9721 ctgtgccagc agagtgagat gcagcagcaa ccgtcaggca atccgtttga tcagtcttct
     9781 cagccgcagc aacagccgca acagcaacct gctcagcaag agcagaaaga cagcgacggt
     9841 gtagccggtt ggattaaaga tatgtttggg agtaattaac gtctaagcgt gagataccgg
     9901 atggcgagct gccatccggt aaaataacat cccctccaaa gtattaaccc tttcttttca
     9961 tctgattgtt tattgaccct tcaggaatgc gcagattgcg taccgcttgc gaacccgcca
    10021 gcgtttcgaa tattatctca tctttataat aatcattctc gtttacgtta tcattcactt
    10081 tacaccagag aaataccaat ggcaccttcc aaaactgctc agccaaaaca ctcactgcgt
    10141 aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc acaggcagcg
    10201 gttgaaccga aagaagacac tatcaccgtt accgctgcac ctgcgccgca agaaagcgca
    10261 tgggggccgg ctgcaactat tgcggcgcga cagtcagcta ccggcactaa aaccgatacg
    10321 ccgattcaaa aagtacctca atctatttct gtcgtcaccg ctgaagagat ggcgctgcat
    10381 cagcctaagt cggtgaaaga agcgcttagc tatacccctg gcgttgctgt gggaacccgt
    10441 ggcgcatcta atacttatga ttacctgatc atccgcggat ttgcggcaga cggccaaagc
    10501 cagaataact atctgaatgg cctgaagttg cagggcaact tctataacga tgcggtcatc
    10561 gacccgtata tgctggaacg tgctgaaatt atgcgtggcc cggtttccgt gctttacggt
    10621 aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc gtccgaccac tgaaccgttg
    10681 aaagaagttc agtttaaggc cgggactgac agcctgttcc agaccggttt tgacttcagc
    10741 gatgcactgg atgatgacgg cgtttattct tatcgcctga ccggtcttgc gcgttctgcc
    10801 aatgcccagc agaaagggtc agaagagcag cgttatgcta ttgcaccggc gttcacctgg
    10861 cgtccggacg ataaaaccaa tttcaccttc ctttcttact tccagaacga gccggaaact
    10921 ggttattacg gctggctgcc gaaagagggg accgttgagc cgctgccaaa cggtaagcgt
    10981 ctgccgacag actttaacga aggggcgaag aacaacacct attctcgtaa cgagaagatg
    11041 attggttata gcttcgacca cgaatttaac gacaccttta ctgtgcgtca gaacctgcgc
    11101 tttgcgcaaa ataaagtttc gcaaaagagc gtatatggct acggtatgtg ttcagatccg
    11161 ctgtatacca aagaccagga ggcactcaag gcgagtccat gtttaagtat cccgcagtct
    11221 caatggggcc atacactgac tcgtcagtac gttatcgata atgagaagtt agaaaacttc
    11281 tctgttgata ctcagttgca aagcaaattc gcaaccggtt ctgtggatca cactctgctg
    11341 actggcgttg actttatgcg tatgcgtaat gacatcgact cctggttcgg atatgccggt
    11401 tcagttgcac catctgatat ctataattta gaccgtagtg actttgattt tggcgctcac
    11461 ccgaatccgt ctggtccata ccgcgttctg ctgaaacaga aacaaaccgg cctgtatgtt
    11521 caggatcagg cgcagtggga taaagtgctg gttacgcttg gcggtcgcta tgactgggcc
    11581 gatcagtcat ctttcaaccg tgattacggt aataaatctg aacgtgacga caaagagttc
    11641 acctggcgtg gtggtgtaaa ctacctgttc gacaacggcg taacgcctta cttcagctac
    11701 agtgaatcat ttgaacccgc ttcacagact gatgcgaacg gtgatctgtt tgcaccttcg
    11761 aaaggcaaac agtatgaagt cggcgtgaaa tatgtgccag aagatcgccc aattgtggtg
    11821 actggcgcac tgtatcagct taccaaaact aataacctga tggcggatcc gaacggttca
    11881 ctgttctcgg ttgaaggcgg tgagattcgt gcgcgtggcg tagagctgga agcgaaagcg
    11941 gcactgtcgg cgagtgttaa cgtagtcggt tcttatactt acaccgatgc ggaatacact
    12001 accgatacta cctacaaagg caatacgcct gcacaggtgc caaaacacat ggcttcgctg
    12061 tgggctgact ataccttctt tgacggtccg ctttcaggtc tgacgctggg caccggtggt
    12121 cgttatactg gctccagcta tggtgatccg gctaactcct ttaaagtggg aagttatacg
    12181 gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg gcatggcggg ctccaacgtg
    12241 gcgctgcatg tcaacaacct gtttgatcgt gaatacgtcg ccagctgctt taacacttat
    12301 ggttgcttct ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt
    12361 ctcttttggg gcacggattt ccgtgcccgt ttcacaagtt ggctgttatg caggaataca
    12421 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12481 cgcttttgca tccgttgtcg ttaacctttc ctgccgggaa agtgactggt ctgattggtc
    12541 acaacggttc tggtaaatcc actctgctca aaatgcttgg tcgtcaccag ccaccgtcgg
    12601 aaggggagat tcttcttgat gcccaaccgc tggaaagctg gagcagcaaa gcgtttgccc
    12661 gcaaagtggc ttatttaccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12721 tggtggcgat tggtcgttac ccgtggcatg gcgcactagg gcgctttggt gcggcagatc
    12781 gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    12841 tcgatagcct ttctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    12901 atagccgctg tctgttgctc gacgaaccga cttcggcgct ggatatcgcc caccaggttg
    12961 atgtactggc gctggtgcat cgtttaagtc aggagcgtgg cctgacggtc attgccgtgc
    13021 tgcacgatat caatatggcg gcgcgctact gtgattatct ggtcgccctg cgcggcggtg
    13081 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt
    13141 atggcatccc gatgggtatt ctgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13201 attgatgagc ggcttacctc ttatttcgcg ccgccgactg ttaacggcga tggcgctttc
    13261 tccgttctta tggcagatga ataccgccca cgcggcagtt attgatccca atcgtattgt
    13321 ggcactggag tggttgccgg tggagttact gctggcgctc ggcatcgtgc cttacggcgt
    13381 ggcggacacc atcaactatc gcctgtgggt cagcgaaccg ccattgccgg actcagtgat
    13441 tgacgttggt ttgcgtacag aacccaacct tgaactgctg accgaaatga aaccgtcgtt
    13501 tatggtctgg tctgcaggat atggcccttc accagaaatg ctggcacgta ttgcgccggg
    13561 gcgcggattt aacttcagcg acggcaaaca gccactggcg atggcgcgta aatcactgac
    13621 ggaaatggca gatttactta acctgcaaag cgcagcggaa acgcatttaa cgcactatga
    13681 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct
    13741 gacgacgctt atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga
    13801 aattctcgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    13861 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    13921 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    13981 gtatgttcgc gccggacgct ttcagcgcgt tccggcggtc tggttctatg gtgcgacgct
    14041 ttctgcaatg cactttgtgc gtattctgga taacgccatc ggaggtaaag cgtgagtaaa
    14101 cgaattgcgc tttttccggt gttattgctg gcgctgctgg tggttgctgc tgcggcgttg
    14161 acctggatga acttctcgca ggcgctgccg cgcagccagt gggcgcaggc cgcctggtcg
    14221 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg
    14281 atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg tgctgtttca gcaagtgctg
    14341 cgtaacccgc tggcggaacc gacgacattg ggtgttgcaa caggcgcgca actggggatt
    14401 accgtcacca cgctctgggc aatccccggc gcgatggcga gccagtttgc tgcgctggca
    14461 ggggcttgtg ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg tctttcgccg
    14521 gtaacgctga tcctcgcggg gctggtagtg agcctttatt gcggcgcaat caatcagtta
    14581 ctggttatct tccatcatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg
    14641 acgcaaaccg actggggcgg cgttgaacgt ttatggccgc agctgctggg cggcgtgatg
    14701 ctgacgttat tgctacttcg cccgttaact ctgatggggc ttgatgatgg cgtggcgcgc
    14761 aatctcgggc tggccttgtc gctcgctcgt ctggcggcgt tgtcgctggc gattgtcatc
    14821 agtgcgctgc tggtgaacgc ggtggggatt atcggcttta tcgggttgtt cgcgccactg
    14881 ctggcgaaaa tgctggggac gcggcgtttg ttgccacggt tgatgctggc atcgctgatt
    14941 ggtgcgttaa ttctgtggct ttctgatcaa atcatcctct ggctgactcg cgtgtggatg
    15001 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggttgctg
    15061 ccgcgtttac gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcgactgaa
    15121 cgccaacacg tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg
    15181 gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc
    15241 gaggatttga tgccctggcg ctggccgcga attatggctg cgctgtttgc gggcgtcatg
    15301 ctggcggtgg cgggctgtat tattcagcgg ctgaccggaa acccgatggc aagcccggaa
    15361 gtgctgggga ttagctccgg cgcggcgttt ggcgtggtgt taatgctgtt tctggtgccg
    15421 ggtaatgcct ttggctggct gttacccgca ggcagtctcg gggcggcggt gacgctgttg
    15481 atcattatga tcgccgccgg acgtggtgga ttttccccac accgcatgtt actggcggga
    15541 atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg
    15601 cgaatggcgc aagttctgac ctggatttcc ggttcgacct acaacgcgac cgatgcgcag
    15661 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtgccgccgc
    15721 tggctgacca ttttaccgct gggtggtgat accgcacgag ccgtggggat ggcgctgacg
    15781 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacaatgacc
    15841 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt
    15901 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtgttc
    15961 gctgactggt gtgggcggat ggtattgttt ccattccaga tcccggcggg gctgctgtcg
    16021 acctttatcg gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaatagaat
    16081 gcctcgcccg gtgggcgagg caaaatcatt acaacttcgc aaacacccga cgtgcagcat
    16141 cgatggtgtt attgatatct tccatgctat gcgccacgga cataaagccc gcttcaaacg
    16201 ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt
    16261 ccacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa
    16321 taccgaacat gccgccaacg tggtttacca ccagcggaat tccggcttct tctgccgctt
    16381 cccgcagacc ttctgccaga cgtgttgtca gctcatccag cgtctcgtga acgcccggct
    16441 gcgcgacttc attcagacag gcgaaaccgg ctgccatcgc aatcgggtta ccggaaagcg
    16501 tacccgcctg atagaccgga ccggtcgggg ccagcgcatc cattacatca cgacgaccac
    16561 cgaatgcgcc taccggcatt ccaccgccga tgattttgcc gaggcaggtg agatccggtt
    16621 ccacaccgta ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat
    16681 cgatgatcag caacgcgcca aattcgtcac acagcgcacg cagacctggc aggaactctg
    16741 gcagcggtgg aacgcagttc atattgcctg ccaccggctc gacgataata caggcaatct
    16801 cttgcgggta ttgctcaaac gcggcgcgta cagaggccag atcgttataa gtacaggtta
    16861 aggtatgttt ggcgaaatct gccggaacgc ccggcgaatt tggctggcct aacgtgagtg
    16921 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    16981 taataatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgctt
    17041 cagtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17101 gcgccatttt cacttccatt tcggttggtg caccaaagct taaaccacgc tcggcggctt
    17161 caatcacggc attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac
    17221 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17281 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17341 cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17401 gattcgtaga aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg
    17461 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17521 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17581 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17641 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17701 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct
    17761 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcactggta
    17821 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg
    17881 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc
    17941 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg
    18001 gtgaaattct ttggcgggct gggaacactc ggcggaggca tggtgttggg gcgcgaaggg
    18061 ccaaccgtgc agataggcgg caatatcggg cggatggtac tcgatgtttt ccgcctgaaa
    18121 ggtgacgaag ctcgtcatac gctgttggca accggtgctg ctgcggggct ggctgcggcc
    18181 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18241 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18301 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18361 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18421 aataaatggg tgctggggat gcaggatttg ctgcactgtg tgcacggtgg caatattacc
    18481 aaatgggtac tgatgggtgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18541 ccagcaactt cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc
    18601 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact ctgcttctct
    18661 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18721 gctttcggaa tggttgccgt tgagttgttt ccgcaatatc accttgaggc ggggacgttt
    18781 gctattgccg gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc
    18841 attctggttc tggaaatgac cgataactac cagctcattt tgccaatgat tattaccggt
    18901 cttggcgcaa cactattagc acaatttacc ggtgggaaac cgctatactc ggcgattctt
    18961 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19021 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19081 aatttgccca attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19141 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19201 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19261 ttcacctttg atgatcaggt gaatgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19321 ctggtggttg atccgatgag cctgcaatat ctggtcggcg gttccgttga ctataccgaa
    19381 ggtctggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagtac ctgcggttgc
    19441 ggttcttcct ttagtatcta atctgctgtt catgtgccgg ataaactatc cggcacatct
    19501 cctctcttca acgaccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19561 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19621 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagtgg
    19681 cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgatcc
    19741 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    19801 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    19861 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    19921 tgaccattgc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    19981 gcgccatgtc gcgaattgtt ccgccgccta ctgcggtaac cacgcccaat accagaacgc
    20041 caaaagggtc catacgcaat tttccggcta acaaaacgcc agagatagca aataccgctg
    20101 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag
    20161 gtcaggcact aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata
    20221 cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
    20281 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc
    20341 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct
    20401 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca
    20461 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
    20521 tattgcgctt tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggcctta
    20581 tccggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
    20641 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
    20701 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
    20761 ttcatcccct gccaggtgga aacctgccca atcttttgcg cttgtggagg atagtcggaa
    20821 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
    20881 gaaagcgtga tgacgcgcgg cgcggcattg agccacagcg gcgcgagaaa agacagggcg
    20941 accagcgccc tgaacagtga cttagccatg tgccagtttc tgcaccagtg actcaaccat
    21001 caggctggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
    21061 atcggccacg tcggagatgg cgcgtactac gacaaacggg acgttgaaat tgtggcagac
    21121 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
    21181 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
    21241 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
    21301 tttgaagcct gccgggcagc ctggtaactg accgtattca taaccaaatg ccgtgacatc
    21361 cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc
    21421 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa
    21481 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
    21541 tccattcagt tggccggtat agatttcgca accgcccaga ctgatagttt gacggttttc
    21601 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
    21661 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
    21721 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
    21781 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagattctg cggatcttcg
    21841 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
    21901 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
    21961 tccagcaggt ggggcgctac atcgctaaag aaattttaag ccgtttgaaa gagcttaaat
    22021 tactggaggc gtatggtctc gatgaactga ccgggccctt tgaaagcatt gttgagatgt
    22081 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa
    22141 atgactggtt tcgccagcgt ttgtacccgg aagatgccga aagccagcct ctgactgacg
    22201 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgcgctgc
    22261 ggcgcaagat tcgtcaggac ttatgtcatt ttgaaggtaa cgcacagggt attcgtttgg
    22321 tgcatacatt gatgcggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata
    22381 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
    22441 cgggctatta tctttccgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg
    22501 cgctttacag tcgttttccg ttaacgtgga ttatggaagc tgccgacgac atctcctatt
    22561 gtgtggcaga ccttgaagat gcggtagaga aaaggatatt taccgttgag cagctttatc
    22621 atcatttgca cgaggcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg
    22681 aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt
    22741 ttatgtattt acgggtaaac accctaaata aactggtacc ctatgcggca caacgattta
    22801 ttgataatct gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca
    22861 gcgaatgcag cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc
    22921 atccagatgt cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga
    22981 tttatcgtcc tttattaaac ctgccgttat cagactttac tgaactggta gaaaaagaac
    23041 gggtgaaacg tttccctatt gaaacgcgct tattccacaa actctcgacg cgccatcggc
    23101 tggcctatgt cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg
    23161 aatattatta ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg
    23221 cgtgggatga ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg
    23281 aacaataaat ttttaccttt tgcagaaact ttagttcgga acttcgcgct ataaaacgaa
    23341 tctgaagaac acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa
    23401 ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg
    23461 caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga
    23521 tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta
    23581 atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc
    23641 aggagggttc tccgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg
    23701 gcggccagca acagaaattc atggcgctgg gttccggcgt tatcattgat gccgataaag
    23761 gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccaactga
    23821 gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc
    23881 tgatccagat ccagaacccg aaaaacctga ccgcaattaa gatggcggat tctgatgcgc
    23941 tgcgcgtggg tgattacacc gtagcaattg gtaacccgtt cggcctgggc gagacagtaa
    24001 cttccgggat tgtctctgcg ctgggacgta gcggcctgaa cgctgaaaac tacgaaaact
    24061 tcatccagac tgatgcagcg atcaaccggg gtaactcagg tggtgcgttg gttaacctga
    24121 acggcgaact gattggtatc aacaccgcga ttctcgcacc ggacggcgga aacatcggta
    24181 tcggttttgc tatcccgagc aacatggtga aaaacctgac ctcgcagatg gtggaatacg
    24241 gccaggtgaa acgtggtgag ttgggtatta tgggcactga gctgaactcc gatctggcga
    24301 aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg ccgaattctt
    24361 ccgccgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccaa
    24421 tcagcagctt tgccgcactg cgtgctcagg tgggcactat gccggtaggt agcaaactga
    24481 ccctgggctt actgcgcgac gggaagcagg ttaacgtgaa cctggaactt cagcagagca
    24541 gccagaatca ggttgattcc agcaccatct tcaacggcat tgaaggcgct gagatgagta
    24601 acaaaggcaa agatcagggc gtggtggtga acaacgtgaa aacgggcact ccggctgcgc
    24661 agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca
    24721 tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc
    24781 gcggcgacag caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaaaac
    24841 gggaaggggt tctccttaca atctgtgaac tttaccacaa ctccatacat cttcatcatc
    24901 ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataat
    24961 ggaggactta tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt
    25021 accatgcgca tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc
    25081 agcggcgatc gtgagcgtat tggtgaattg cacgaaggag cattgctggt actttcacaa
    25141 ggacgagtcg tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcagggg
    25201 attaatctac cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa
    25261 ccagagaatc tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa
    25321 cagtcgcggt tgatgcacct gttggcgcag gatagccgtt tgcgggaaga actggtgatg
    25381 aacctcattc aggctgagga gaatactccc gcacttactg aatgggcgca acggctgggg
    25441 atcgatctca atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggc
    25501 gtggacagcg caatggcgga gttacagcaa ctgcaaaatg cgctgactac gcccgagcgt
    25561 aataatctgg tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac
    25621 tcttttgggc gctgggacgc agaagatcat cgtaagcgag ttgaacaact gattacccgc
    25681 atgaaagagt atggccagct gcgttttcgc gtttcgctgg gcaactattt taccggtcct
    25741 ggcagtattg cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg
    25801 atgccagaaa gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt
    25861 ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg
    25921 gacaataacg gcttgctgcg gcgaacgctg gcggcgtggt ttcgccacaa tgtgcaaccg
    25981 ctggcaacgt caaaggcgtt gtttattcat cgtaatactc tggaatatcg gcttaatcgt
    26041 atatcggaac tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg
    26101 gcgttacaac tggatgaaga gcggtaggtc gattacaaaa ttacagccgg gtgagacttt
    26161 acccggccca cagaatggca ttatttacgc gtcagttttt ccagatcggc ttcaatctcg
    26221 ctgatcttat tggttactac tgactccagg tgacgtaagt cgtcgaggat cttacgctta
    26281 agatcaactt cgctgcggtc acgctggcag atttgatcaa gctcatcaat gatataccgt
    26341 agattcgggc taatttcctg gacttcttta taaccctgac ccacaccatc agcgacgacc
    26401 gttttacgct gacgcgggta tttaaacttc acgctcttgg cgaaaaactc gcctttgtct
    26461 ttctggaaat agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca
    26521 atttcttcag gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa
    26581 aatgagtcag taaattgtgc ttattttagc atttggccag gcccgcccgg ctcgactgtg
    26641 atcgcagaaa gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct
    26701 gctttattgt ggtgataaaa ttcacgttct acaccagttc cagcatcgtt acttccgggc
    26761 ggcagttcag acgcaaacca tacaaactgc ccacgccacg ggttgtgtag atgtgtcgtt
    26821 cgccaaaggc atttaatccg gcgacgtaac gtttatcttc gacaggggca aaaggttcac
    26881 caaccaacgg tacgcgcagt tgcccgccgt gagtatggcc gcacagcatc agatcccagg
    26941 gatcatcacg catgacttct ttgctgtcag gattatgcgc cagcaccagt cttggcagat
    27001 tggctttgct ggcgggaggc ggtttgcatt gtccggccca taagtcaccg gtgcctacca
    27061 gttcgaattg cctgttcggc gtggcgatca ccgtagcctg gttaaacaac accgtgatgc
    27121 ccactgattt caacgtctcg ccaattaagt gatttttttc tgtaccaaca gggcgatcgt
    27181 ggttgccgaa gcaggcaaac gtcggcgcac attcggcaag gggggagagt acgtcactaa
    27241 acgccgaaaa attcagcggc atatcaaata atacgtaatc gccgcccagt aatatcaaat
    27301 cgggcttttg ttctatgcca agagcaatcg cgtcagaaat caggcttaaa ggaacaaaac
    27361 gagagtaatg gagatcggcc agaaaaagaa ttttgaatgg tgctgcgttg tctttaaaaa
    27421 aggcgaggcg gtgacggatt aattcaaacc agcctggctc ccagtaatgc atataaccga
    27481 aacctgagcc cgtggctatc gtcgcggcag tagcctgcaa aaatcggcgg cgtgaaatca
    27541 tcgctcatcc ctgcaaaaaa atcgggcagc gtcgtgctgc ccgtgtacat acttttagtc
    27601 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    27661 aacgataacc gcacagtaaa gactgtattt gccatctttc gacggcagat tgcctgaaac
    27721 aaccacagat cccgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    27781 acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt cgacaatcac
    27841 cccttcaacc acttcagagc gtgcgccgat gaagcagtta tcttcaataa tggtcgggtt
    27901 agcctgcagc ggctccagca cgccgccgat acccacgcca ccggaaaggt ggacgttttt
    27961 accaatctgc gcacaggaac cgacggtcgc ccaggtatca accatggtgc cttcatcaac
    28021 atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa tgaacgcgcc
    28081 ctgacgcact gccgctggtg gcacgacgcg gaagccttct ttctggaaac gtgcttcatc
    28141 gtagttagcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac
    28201 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    28261 ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac
    28321 ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct cggcacggcg
    28381 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    28441 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    28501 ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga ataaatcttc
    28561 tactcgctcg ccaatggttg taattcgggc gccatgaagc gaaattccca gatcggcaaa
    28621 aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga
    28681 tttgcggtcg gtatgggtcg gcaaaaacgt cacttcggtt tcaacagtaa aatggcgcaa
    28741 tttggcaggt tggcgacgcg gctgcggtgg ctgccagcta ctttgtgtca gcacctgctc
    28801 cagaccaaac cgaataacct catgacgatc ggcggacagc gggctgccat cgggttccag
    28861 tacgatgaag gtatccatcg ccataccgtc acgagtggtg aaaatttgtg cgtcgtgaac
    28921 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    28981 gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca gtaccagcgg
    29041 tttgcttaaa tcatgctgta ataaatggcg tgcgtgccag gccagttgat ttgggctatg
    29101 gcggacaaaa tagttagcgc gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    29161 gttatccatg cgcagtagtg ccagcgcctg gagttggtga tggcgaaccc gttcgcgcat
    29221 atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg
    29281 caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa tgtcagccac
    29341 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    29401 gatgacttcc gggtcctgaa tatcgcggcg ctgagctgtc accgacatca gcaagtgctg
    29461 gcgaaccagc caggcgacca gctgtgtttc gcgggagttc agcccgtgga gttcggcaaa
    29521 atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    29581 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    29641 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    29701 gcggatagta tgttcatcca ccgtgtaggc atggaacagg tcaaactgca tctgcccgac
    29761 gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca ttggcaatag
    29821 cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc gcgcttccgg
    29881 aatattacac agaggttgtt gcaggtggcg acgggcgtgg cgtaactggc gtagtgtggt
    29941 ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    30001 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg atcagcgttc cgcgtagctg
    30061 aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    30121 gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc
    30181 cttcatcatc cgctcgaccg gttcgttacc ttcaccacta taattcaaac gctgggcgac
    30241 gctaagctgg cgatcgaata acaggcgatt atcgtaacga ctgacgacca gatgcagggc
    30301 aaagcgaatg cgccacaata tatgcaggca ttcgtttaat tccgctctct ccgcagaggt
    30361 taagaagcca aaccctacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac
    30421 ccattgcaga gtgtggatat cgcgcaagcc gccggggcta cttttgatgt ctggttcaag
    30481 gttgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc
    30541 gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca gttcgaggaa
    30601 cagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    30661 cgataaccct tccagcatgc actcttcaag cgtgcgcacg ctatgaccta cttccagctt
    30721 tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt
    30781 tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta
    30841 gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa
    30901 tcgttgaaga agctggtcga taaactcggt acgcgcctca atcaactgtt ctgcggagat
    30961 accattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc
    31021 accgacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac cgtgcagggt
    31081 ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga
    31141 ttaaattatt gcattaaaaa agccggcatt cgccggcttc attttattcg tcgtgcgaga
    31201 ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca
    31261 ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc
    31321 catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg atggtgaacg
    31381 tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc acctgcggtt
    31441 cttcgtggaa gccgcgacca ataccgtgtc cacaatattc acgaacgacg gagaagcctt
    31501 ctgcttcgac aaatttctga attgccgcac cgatttcgcg cagattaatg ccaggtttta
    31561 ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt tcgcccatga
    31621 tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta
    31681 cggtgacatc aatgttaacg atatcgccat ctttcagcag cttggcgtcg tccgggatac
    31741 cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg tgatagccga
    31801 ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct
    31861 cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca
    31921 gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta
    31981 attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc
    32041 acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aaggttttct
    32101 ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg gtgtccacga
    32161 cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg
    32221 gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc
    32281 ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt
    32341 taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc
    32401 agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc
    32461 acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca
    32521 agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag
    32581 cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg
    32641 gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa
    32701 ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc
    32761 gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg
    32821 acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagag gcaaacaacc
    32881 tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg
    32941 ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg
    33001 caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag
    33061 aagctgagta ataaggcacg ctcgtacgag cccttataga ccaggtagta cacgtttggt
    33121 tagggggcct gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc
    33181 agatcacatc tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct
    33241 gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg
    33301 cgacatcgag ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa
    33361 agcaggcaac gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat
    33421 cattctggaa gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt
    33481 cgcagacaaa gttctggacg cagctgttgc tggcaaaatc actgacgttg aagtactgaa
    33541 agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat
    33601 tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat
    33661 cggtgttctg gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca
    33721 cgttgctgca agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga
    33781 aaaagaatac caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc
    33841 agagaaaatg gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca
    33901 gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga
    33961 agtgactggc ttcatccgct tcgaagtggg tgaaggcatc gagaaagttg agactgactt
    34021 tgcagcagaa gttgctgcga tgtccaagca gtcttaatta tcgaaaagga gccgcctgag
    34081 ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa
    34141 aagcgacgta caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga
    34201 aacatggcta ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa
    34261 gctctgcagg gcactgaagg cttcggtatt gatgcaagca tactggatcg catggctcag
    34321 gaaatcaaag aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac
    34381 ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg
    34441 gggatgctgg cgaccgtaat gaacggcctg gcaatgcgtg atgcactgca ccgcgcctat
    34501 gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg
    34561 gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt
    34621 aacccgttct ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat
    34681 gtggtgctga aagcaaccaa agttgacggc gtgtttactg ctgatccggc gaaagatcca
    34741 accgcaacca tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc
    34801 atggacctgg cggccttcac gcttgctcgt gaccataaat taccgattcg tgttttcaac
    34861 atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc
    34921 acggaataat tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcggggga
    34981 tcttacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    35041 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    35101 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    35161 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc
    35221 cgacgccgct gcgtcagctg gcaagcgtaa cggtagagga ttcccgtact ctgaaaatca
    35281 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg
    35341 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35401 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35461 tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    35521 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    35581 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    35641 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    35701 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    35761 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    35821 gcgctggtgg caggcaaaaa tgtcgctcgc atggtagaac agtgcctgga attctctccc
    35881 cgctatgctg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    35941 cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt
    36001 gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt
    36061 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    36121 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36181 gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36241 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36301 gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac
    36361 tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36421 tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac
    36481 ccgcagtcag tgattcactc aatggtgcgc taccaggacg gcagtgttct ggcgcagctg
    36541 ggtgaaccgg atatgcgtac gccaattgct cacaccatgg catggccgaa tcgcgtgaac
    36601 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    36661 tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg ccaggcagcg
    36721 acgacagcat tgaatgccgc aaacgaaatc accgtggctg cttttcttgc gcaacaaatc
    36781 cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa
    36841 ccacaatgtg tggatgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa
    36901 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta
    36961 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    37021 tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac
    37081 cgtaaaccct gtcagggaat aagaaacgcg tgatgttgtc tgctactcaa ccacttagcg
    37141 aaaaattgcc agcgcatggc tgtcgtcatg ttgcgatcat tatggacggc aatggtcgct
    37201 gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca aaatccgtcc
    37261 gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta
    37321 gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt
    37381 gggcgctaga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg
    37441 gcgataccag tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa
    37501 cagccgggaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata
    37561 tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc
    37621 agatagatga agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt
    37681 tagtaattag gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct
    37741 atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa gactttgaag
    37801 gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg
    37861 aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct tttgtgttaa
    37921 tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc attgtaacgc
    37981 tggtggtctg tatgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt
    38041 cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt
    38101 tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgc
    38161 tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct
    38221 ggcgtaactc taaaacattg cgcattattt ttggcgtgct aaccattgtt cccttcttct
    38281 ggggcatgct ggcgttacgg gcctggcact acgacgagaa tcattacagt ggcgcaatat
    38341 ggctgctcta tgtcatgatc ctggtatggg gcgctgactc tggcgcatat atgtttggca
    38401 aattgtttgg taaacataaa ctagcaccga aggtttctcc gggtaaaacc tggcaaggct
    38461 ttatcggtgg actagtgacc gcagcggtaa tctcatgggg ttatggcatg tgggtgaatc
    38521 tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc
    38581 tcggcgatct gaccgaaagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt
    38641 taattccagg acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg
    38701 tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag
    38761 ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga
    38821 atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg
    38881 gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg ttatcgccct
    38941 gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg ttgttccgga
    39001 actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga ttattgccgc
    39061 aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg
    39121 tgtgcctggc gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc
    39181 acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga
    39241 tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacggtagc
    39301 gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga
    39361 gcctgataaa gaagatccgg tatcttcgtt ggggattcgt cctcgtgggc cgcaaattga
    39421 acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg
    39481 cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct
    39541 tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt
    39601 gtctttgaca ttaatcccgg agagtaaacc gggtaagggt aaagcgattg gttttgtcgg
    39661 tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc
    39721 gttcaacgct atcgtcgaag ccacggacaa aacgtggcag ttgatgaagc tgacggtcag
    39781 tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc
    39841 tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct
    39901 tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg
    39961 ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt
    40021 tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact
    40081 tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg
    40141 atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa
    40201 gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggcc
    40261 ctcctcagta tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc
    40321 attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat
    40381 acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac
    40441 aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc
    40501 gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac
    40561 agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc gcgcaaccgt
    40621 gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt
    40681 gttggtaacc atgctttcac caccgatgaa ctgatctctc atttccaact gcgtgacgaa
    40741 gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga aacagaaact tgcgggcgac
    40801 cttgaaaccc tgcgcagtta ctatctggat cgcggttatg cccgtttcaa catcgattct
    40861 acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa catcaccgaa
    40921 ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct
    40981 gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc
    41041 aaaatggaag atgacattaa aaagcttctt ggtcgctatg gttatgccta tccgcgcgta
    41101 cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgtgt gaacgttgat
    41161 gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat
    41221 gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggctggggag cgatctggtc
    41281 gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc
    41341 caacgcgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa agaacgtaac
    41401 accggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct
    41461 ggtgtgcaac aggataactg gttaggtacg ggttatgctg ttggtatcaa cgggaccaaa
    41521 aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac cgtagatggt
    41581 gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc
    41641 gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat
    41701 aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag
    41761 gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat
    41821 aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac
    41881 cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattccg
    41941 ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgattgat
    42001 gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga tggtttaggc
    42061 ggcaaagaga tgccgttcta tgagaacttc tatgccggtg gttccagcac cgtgcgtggc
    42121 ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat
    42181 gatccggact atgattacga atgtgcgact caggacggcg cgaaagatct gtgtaaatcg
    42241 gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg
    42301 ccgtttatta gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt
    42361 accgtttggg atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat
    42421 ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg
    42481 ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc
    42541 cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta
    42601 gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg
    42661 gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg caggtctcgg
    42721 tttagcactg gcaacatctg ctcaggcggc tgacaaaatt gcaatcgtca acatgggcag
    42781 cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa
    42841 aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct
    42901 gcagtccatg aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg
    42961 ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga
    43021 agaacgcggc aaactggtta ctcgcatcca gactgctgtg aaatccgttg ccaacagcca
    43081 ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga
    43141 catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga
    43201 tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt
    43261 tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg
    43321 tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt
    43381 cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca
    43441 aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc
    43501 gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg aatccggcgt
    43561 tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat
    43621 cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca
    43681 gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga
    43741 tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg attgcgtgga
    43801 gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg
    43861 cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc
    43921 agttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg
    43981 cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat
    44041 gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca
    44101 acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa
    44161 gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc
    44221 gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt
    44281 ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa
    44341 gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat
    44401 tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc
    44461 cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca
    44521 caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac
    44581 tatttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg
    44641 atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct
    44701 ctggtcgacg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc
    44761 tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg
    44821 tcgattggcg cgaacgctca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt
    44881 ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc
    44941 gataatgaga tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct
    45001 ggcgaaccga cccgcgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt
    45061 catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg
    45121 atcaatgcgc acattgcgca cgattgtacg gtaggtaacc gctgcatcct cgccaacaac
    45181 gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca
    45241 gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc cggtgtggcg
    45301 caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat
    45361 atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg
    45421 tataagctga tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa
    45481 ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc
    45541 ggtctgattc gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa
    45601 cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg
    45661 cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcctggtacg
    45721 aaatggaaga actggcagtg atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac
    45781 tacatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg
    45841 gtattgatgc gcctgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca
    45901 aaaccattca ttacgtcagt ccgtccgtct gggcgtggcg acagaaacgc gttttcaaaa
    45961 taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca
    46021 aatacaacgt accgtgccgc tttatcggtc ataccatggc tgatgccatg ccattagatc
    46081 cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac tgtctggcat
    46141 tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc ctgaaaacgg
    46201 cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg gtgaatgcca
    46261 aacgccgcga gcagtttgaa cgcatcaaag ctgcagtcgc gccagacctg tcggttcatt
    46321 tgctggatgg aatgggccgt gaggcgatgg tcgccagtga tgcggcgcta ctggcgtcgg
    46381 gtacggcagc actggagtgt atgctggcta aatgcccgat ggtagtggga tatcgcatga
    46441 agcctttcac cttctggctg gcgaagcgac tggtgaaaac tgattatgtc tcgctgccaa
    46501 atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt gagccgcaaa
    46561 aattggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccat gcgatgcacg
    46621 acaccttccg cgagctgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag
    46681 ccgttctgga gttagcacaa tgatcgaatt tgtctatccg cacacgcagc tggttgcggg
    46741 tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct
    46801 tgacccggcg cgcccaattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg
    46861 tctggcgctc tgtgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgtgcgga
    46921 acctcacgaa atcgacgaac tgaatatttt acatgcgact atgctggcga tgcagcgtgc
    46981 cgtcgctgga ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa
    47041 attaccgatg ccttcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc
    47101 cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt
    47161 cccgcaatat ggttttgccc aacacaaagg gtatccaacc gcttttcatc tggaaaaact
    47221 ggctgaacac ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact
    47281 gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga
    47341 accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa
    47401 aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga
    47461 tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa
    47521 gcccatcgtc ggggcggatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca
    47581 cctgacggtg ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa
    47641 agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga
    47701 attaaatgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgttg gacgcagtct
    47761 tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc
    47821 ggatcgctat tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca
    47881 cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta acgatgtgcg
    47941 ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt
    48001 taccctcgac gatcctaaac gccctcgtaa ctattcgccg cagcaatata tgcgtagcga
    48061 agaggagatg tgtgagctgt ttgccgacat ccccgaagcc cttgccaaca ccgttgagat
    48121 tgccaaacgt tgtaacgtaa ccgtgcgtct gggtgaatac ttcctgccgc aattcccgac
    48181 cggggacatg agtaccgaag attatctggt caagcgtgca aaagaaggcc tggaagagcg
    48241 tctggcattt ttattccctg acgaggaaga acgtcttaag cgtcgtccgg agtatgacga
    48301 acgtttggat actgaacttc aggttatcaa ccagatgggc ttcccgggtt acttcctcat
    48361 cgttatggaa tttatccagt ggtcgaaaga taacggtgta ccggtagggc caggccgtgg
    48421 ttctggtgca ggggcactgg tggcctacgc gttaaaaatc accgatctcg atccgctgga
    48481 atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctctatgc ctgacttcga
    48541 cgttgacttc tgtatggaga aacgtgacca ggttatcgaa catgtggcgg acatgtacgg
    48601 tcgcgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag cggtgatccg
    48661 cgacgtaggc cgtgtattgg ggcatccgta cggatttgtc gatcgtatct cgaaactgat
    48721 cccgcccgat ccgggtatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga
    48781 aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg
    48841 ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac
    48901 tgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc agtttgataa
    48961 aagcgacgtt gaatacgccg ggctggtgaa gttcgacttc ctcggtttgc gtacgctcac
    49021 catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc
    49081 gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc tgcaacgctc
    49141 ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct
    49201 gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt
    49261 gcaatcaggg atggtggata actttatcga ccgtaaacat ggtcgcgaag agatctccta
    49321 tccggacgtg cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat
    49381 catcctgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt ataccctcgg
    49441 tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca
    49501 gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa
    49561 aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc actcggcggc
    49621 ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat
    49681 ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga
    49741 gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc tttaccattt
    49801 ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gcgatcaaag gggtaggtga
    49861 aggcccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt
    49921 tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat
    49981 catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg
    50041 cgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat
    50101 gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc
    50161 gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggtctgt acctgacggg
    50221 acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa
    50281 agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc
    50341 gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc
    50401 cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga
    50461 aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct
    50521 taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg
    50581 gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca
    50641 gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga
    50701 tgcacgcgcg cgattgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt
    50761 aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag
    50821 gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa
    50881 aatcgattct ctgaccgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga
    50941 agaagtgcat cgtttgcgtg aaaaaagcgt agaactgacg cgtaaaatct tcgccgatct
    51001 cggtgcatgg cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta
    51061 cgttcgcctg gcatttgatg aatttgacga actggctgga gaccgcgcgt atgcagacga
    51121 taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga tcattggtca
    51181 tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga
    51241 aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgttttaaga tgcctatcat
    51301 cacctttatc gacaccccgg gggcttatcc gggcgtgggc gcagaagaac gtggtcagtc
    51361 tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac
    51421 ggttatcggt gaaggtggtt ctggtggtgc gctggcgatt ggcgtgggcg ataaagtgaa
    51481 tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg
    51541 gaagagcgct gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct
    51601 gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggtg ctcaccgtaa
    51661 cccggaagcg atggcggcgt ctctgaaagc gcagctgctg gcggatctgg ccgatctcga
    51721 cgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggcta
    51781 cgcgtaatcg gcaaatgttc tgaaaagggt cacttcggtg gccctttttt atcgccacgg
    51841 tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac atcattgcca
    51901 ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc
    51961 tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg ctgaagttta
    52021 tcgaacataa cccacgaatt tgcggcgtga tttttgactg ggatgagtac agtctcgatt
    52081 tatgtagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc
    52141 actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg
    52201 cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac gaatatcttg
    52261 ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag cggaagtaca
    52321 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgtc
    52381 tgttttatga ttttttcggc gggaataccc tcaaggcgga cgtctctatt tcggtcaccg
    52441 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
    52501 ggacgtttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
    52561 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
    52621 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
    52681 gtaatgcgtt ggggattctg ggtgggatcc cgcgccgtga atttactcgc gacagcatcg
    52741 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg atcaccaact
    52801 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
    52861 cgattcactt cgattctgcc tgggtgccgt acacccattt tcatccaatc taccagggta
    52921 aaagtggtat gagcggcgag cgtgttgcag gaaaagtgat cttcgaaacg cagtcgaccc
    52981 acaaaatgct ggcggcgtta tcgcaggcgt cgctgatcca cattaaaggt gagtatgacg
    53041 aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt tatcccattg
    53101 ttgcttctgt tgagacggcg gcggcgatgt tgcgtggtaa tccgggcaaa cggctgatta
    53161 accgttcagt agaacgagct ctgcattttc gcaaagaggt ccagcggctg cgggaagagt
    53221 ctgacggctg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc gaatgctggc
    53281 ccgttgcgcc tggcgaacag tggcacggct ttagcgatgc ggatgccaat cacatgtttc
    53341 tcgatccggt taaagtcact attttgacac cggggatgga cgagcagggc aatatgagcg
    53401 aggaggggat cccggcggcg ctggtggcaa aattcctcga cgaacgtggg atcgtagtag
    53461 agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat aaaaccaaag
    53521 caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc aacctgcgga
    53581 tcaaaaatat gctgcccgat ctctatgcag aagatcccga tttctaccgc aatatgcgta
    53641 ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt cccggtttga
    53701 tgttgcgggc atttgatacc ttgccggaga tgatcatgac gccacatcag gcatggcaga
    53761 gacagattaa aggcgaagta gaaaccattg cgctggaaca actggtcggt agagtatcgg
    53821 caaatatgat cctgccttat ccacccggcg taccgctgct gatgccggga gaaatgctta
    53881 ccgaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta gggcaacatt
    53941 accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggt gtttaccgcg
    54001 tacgagtcct aaaaatggca ggataacttg ccagagcggc ttccgggcga gtaacgtgct
    54061 gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata ttgcgattat
    54121 tgcgacggat tatgcgatga gcaaagcttt ctactgcgat attcttggtt tcacgctgca
    54181 aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc ttaatggaca
    54241 atatgtgatt gagcttttct catttccgtt cccgccggag cgccccagcc gaccggaagc
    54301 ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc gatgcggcag tggcgcacct
    54361 tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccctacacgc aaaaacgctt
    54421 cacgttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt aaggcttgtc
    54481 atcgccgcat ttgcccggta acgtgcccgg cattgctact gtaaaatcgc accatcatga
    54541 cactcacgct caatagacaa cttctctcct cacgccagat tctggtggcc tttagcggcg
    54601 gccttgattc tactgtctta ctgcatcaac tggtgcagtg gcggacggaa aatccgggcg
    54661 tcactctgcg cgctatccat gtgcatcacg gtttaagcgc caatgctgat gcctgggtga
    54721 aacattgtga aaacatctgc caacagtggc aggtgccgct ggtggtcgaa cgtgtgcagc
    54781 ttgcgcaaga ggggctaggt attgaggccc aggcgcggca ggcacgttat caggcatttg
    54841 cccgcacctt gttgcccggt gaagtgctgg ttacagcgca acatctcgac gatcaatgtg
    54901 aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg gctatggcgg
    54961 aagtttcgga gtttgccgga acacagctta tccgcccgtt gcttgcccgc acgcgggggg
    55021 aactggtgca gtgggcgttg gcgcatggtt tacgctggat tgaagacgaa agtaatcagg
    55081 atgatagcta cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg cagcagcgtt
    55141 ggccgcattt tgccgaagca acagcccgca gcgccgcact ttgcgctgaa caagagagcc
    55201 tgttagatga actgctggcg gatgatttag cgcactgtca aactccgcag gggacgctgc
    55261 agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatc cgccgctggc
    55321 tggcagggca gaatgcaccg atgccttccc gcgacgcgtt ggtgaggatc tggcaggaag
    55381 tggcgctggc gcgagaagat gcttccccct gtttacgttt gggtgcgttt gaaatccgcc
    55441 gctatcagtc gcaactgtgg tggattaaat ccgtcactgg gcaaagcgaa accattctgc
    55501 cgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaagt gtacagctta
    55561 ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag
    55621 cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag aaaatctggc
    55681 aagagttagg cgttccgccg tggctacgtg acaccacgcc actgctgttt tatggcgaaa
    55741 cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgta ggtgagaatg
    55801 gcgtcagttt tgtctggcag aaaacgctta gtcaagtgaa agccggataa gacgcatcaa
    55861 acgtcgcatc cggcgaaagt caatcaggac tcgctcacca ccaccgtacc gatttccggg
    55921 tggctaaagc tggtaatttt atccagacgc agctcgcggg ttgcaccagc ggcctcgacg
    55981 accagatact ccacattttt gcgggagact aaatcgctgg ccttcgcctg caatttttcg
    56041 ccatctttca gctcaagtgc cagcattaaa tgatgctggc aggcgagctc aagattatcg
    56101 taatcatcac aattgattgg ttgatacgta tcattcattg acataatcgc tcaccagtaa
    56161 gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc
    56221 catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc
    56281 gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc
    56341 tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt
    56401 cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg cacgtattcc
    56461 gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat
    56521 aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg
    56581 ctggcgcgcc atccttcaga aacccaggag cgcatgatgc tgcgcctgct ggcgtggcta
    56641 aaatatgccg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga tgagccggaa
    56701 gcgtggctgc gtaacgatca tttgggtatt gatttgtgga ttgagctggg gctgccggat
    56761 gagcgacgga ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt tgcctataat
    56821 agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgcgca gtttgccaat
    56881 ctttccatct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt
    56941 accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga tgataagaat
    57001 aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg
    57061 ttgctatccc cgatggtgag cttgagatca ccgccattcg tgcgcagggc gcgggcgggc
    57121 agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg gcgtccagcc
    57181 tgccagagta ttacaaagaa cgtctgctcg ccgccagcca tcacttgatc agcagtgatg
    57241 gggtgattgt cattaaggca caggaatacc gcaatcagga gctcaaccgc gaagcggcgc
    57301 tggcccggct ggtggcagtg attaaagatt taacaacaga acaaaaagcc cgacgaccca
    57361 cgcggcccac ccgcgcatca aaagagcgca ggctggcatc gaaagcacaa aaatcaagcg
    57421 tgaaggcgat gcgcggcaaa gtgcgcagcg gtcgggaata aaaagaagga atggatggtg
    57481 aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat gggatgtaat
    57541 aatcgggccg aagtcgatac gctttctccg gcacaggctg ccgaactgaa accgatgccg
    57601 caaagttggc gcggcgtgct gccgtgtgct gattgcgaag gaatcgaaac ctctctgttc
    57661 ctcgaaaaag acggaacctg ggtgatgaat gagcgttatc tgggggctcg tgaagaacct
    57721 tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt attaaccgat
    57781 agcaaaggtg aaaagtcata ttatcgcgcg aaaggagatg cgctggagat gctcgatcgt
    57841 gaaggtaatc cgattgaatc gcagttcaac tatacgctgg aaccggcaca atccagctta
    57901 cccatgacac cgatgaccct gcggggcatg tatttttata tggctgatgc ggcgaccttc
    57961 actgattgcg cgaccggaaa acgtttcatg gtggcgaata acgcagagct ggagcgtggc
    58021 tacctggctg cgcgcggtaa cagtgaaaaa ccggtattac tgtcagtaga aggtcacttt
    58081 acgcttgagg ctaatccgga taccggtgcg ccgaccaaag tattagcgcc cgatacggca
    58141 ggtaaatttt acccaaacca ggattgcagt agtttggggc tgtaacccgt cttgagacag
    58201 aaacaaatga aatattgcct catgcgctgc gcttatcagt cctaaagcat acaatgcaat
    58261 attttgaatt agcacaattt tgtaggccgg ataaggcgtt cacgccgcat ccggcatttt
    58321 gcgcacgagg ctggctttta tcactgagtc agcccaacaa agcggcctgc cttgatataa
    58381 atcccaggct tcatgtgccc aacatactgt aactgcgttt ctggcttaaa cgcagagata
    58441 tcgccagtac gcatatgcat cagatcggca gggctaatcc agcctgattg cgccagcgta
    58501 agcggatgcc cggctttggc aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt
    58561 ttgtctcctt cgcccacact gcttaactgc gctttcgcca ggccgctgac gcactgttga
    58621 cgaaaatcct cggagaacgg atttagtgag cacatctggc gagtcaccat aaatggaata
    58681 aattcgacaa tgccgcgata gttgtaaccg ctatctttaa ttttgttggc gaacgcggtg
    58741 atttctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag cttatcacta
    58801 tgctttatgg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc ttcggcaacg
    58861 ttattatctc ccaaatagat tgcaacgtga ctcacagagg aagtgctgaa tacgcggata
    58921 ccaaatgagg ttaccccaag gcttgaggag aaaagcaaat cgccgggttt gagatctggt
    58981 tccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa tttaacagcc
    59041 catgttttcg cctcggcatt taccactgtg gctgatgagt ctggctggct gatatcaacg
    59101 gtacaggccg aaagtaaaag aaagcagggg agaagcaggc ggcagtacgc ctttggttta
    59161 tccattttat acaatccatg taaaaaaggg ccccgaaatt caggaccctt tctggcatca
    59221 gcctttaatc tgtttcacca gataatcgac gatgtcacca gtcttaatta actgtttctc
    59281 gccgttacga cgatatttat attcgatatc atcgttgtcg aggttacggt cgcccagcac
    59341 aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg gacgctcttt
    59401 gcggtcatcc agcagcactt cgataccttg tgcgcgcagt tcgctgtaca gtttctcagc
    59461 aagttcctgt acgcggaagg atttgtgcat gttcattggc agaatcgcca cctggaacgg
    59521 cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa tcgccgcagc
    59581 taccacacgc gttacgccga taccgtagca acccatcgtc aggatttggt tacggccatc
    59641 ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct ggaagatgtg
    59701 accgacttcg ataccacgtt tgatcagcag cgtaccctga ccatccgggc ttggatcgcc
    59761 agccaccacg ttgcggatat cagcaatttc cggggtagcg acatcgcgat cccagttgat
    59821 accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca tcgccgcaac
    59881 ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac ccggaccggc
    59941 tttaaccacg gcacgaattt cttcttcggt cgcaaaagtc agcgggctgg caacctgcgg
    60001 cagtttttct gctttaactt cgttcagctc gtggtcaccg cgcaccagca gcgcaaccag
    60061 cgggaagcta ctgccttcaa cggctttaac cagcagagtc ttaaccgttt tctcaatcgg
    60121 cagattgaac tgttcaacca gttccgcgat ggtcttcgcg ttcggcgtat caaccagcgt
    60181 catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg ccagttcaat
    60241 gttcgctgca tagtcagaag tatcggagaa gaccacatcg tcttcaccgc tctgcgccag
    60301 cacctgaaat tcgtgagagg cgctaccgcc gatagaaccg gtatcggcct gtacggcgcg
    60361 gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg catcgtaggt
    60421 ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca ggaattcgcg
    60481 ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct ggatctgata
    60541 gaagttcagc ggcagctgtt tgtaagagct cagctcgtta cgaatcaggt ctgtaatcac
    60601 ttcttcatga gttgggccga gtacgaacgg acggtcacca cggtcagcaa tacgcagcaa
    60661 ttccgggcca tactgttccc aacgaccact ctcttgccac agttcagatg gctgaaccac
    60721 cggcattaac acctcgatcg caccggcgtt gttcatctct tcacgcacga tgttttcgac
    60781 ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg ccagcttgcg
    60841 gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc
    60901 cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc agttggaagg
    60961 tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag
    61021 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
    61081 gcgtcggtga cgcgccagcg aacgttaaaa tcatgcagcc agacggcata ggttttgccc
    61141 gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc gcggataaac
    61201 agcgttaact gcggataacg cttcttcagc gtcaaaagct gcttttcgac ctctgcggta
    61261 aaacacaccg ccatttctgc cgctggcgca ctttgcgcat agctggcact ggcatcggga
    61321 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
    61381 tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc tttcagctct
    61441 accagcgaca taccaattgg gttagggcga aaagtggagc gcgtagcgaa aacacccatt
    61501 ctggcattac cgccaagacg aggcggacgc acagtcggac gccagccgcc ttccatcgtt
    61561 tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg aacggcgtcg
    61621 gcctggttgt agggagcaat gagatgtagt tcgccgttgg cgctttttac cagacctggc
    61681 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat ttgctcgaac
    61741 tggaaactgc tcatttcgcc gtgatgttaa gcgcagaacc gatacataca gcctgacgat
    61801 agcagcctgg cgtaccgctg gtgacttcgc agctatgcag taataccgca ttggctttca
    61861 ttttcgaggc gttgatttgc atccgcttac gtgcggttgg aatgctcggc ggagagtcct
    61921 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
    61981 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
    62041 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
    62101 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
    62161 ttcctcaatg tatgatcaaa acgtcaatat tgaatcaggc gcttgtaaaa atgacaagac
    62221 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa tattaccagc
    62281 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttcg tcagactgat
    62341 aagcctgaac gaatttcttc acgttttcgg cgtctttgtt gtcttcacgc gttacgatca
    62401 ggtttacgta cggggactct ttatcttcga caaagatacc gtctttcgct ggagtcaggc
    62461 caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg tcgtccagag
    62521 agcgcggcag ctgcggtgct tccagttcaa caattttcag attttttggg ttctcaacaa
    62581 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
    62641 gcagcagcag cagtgaacga ccaaggttag ttgggtcgtt tggcacggca acctgcgaac
    62701 catcctgcag ttcatccagt gatttgattt tcttggagta acctgcaatc ggataaacaa
    62761 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
    62821 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
    62881 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
    62941 gcgcgacttc tgcaacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
    63001 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
    63061 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
    63121 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac tgaattaaat
    63181 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta tagccgatgt
    63241 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
    63301 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaacgctt
    63361 ccggtaacag cactttacga acgatctgca ttggcgtggc acccattgcg cgggaggctt
    63421 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
    63481 ctgcaccaac ggttaacgga acaatcgctg cctgcaatcc aatcgatgta ccgacaataa
    63541 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
    63601 tcacaatcgc agaaatggta cgatacaact tcgcgttagc aataatttgc cccggacgcg
    63661 tgacataaag cagaacgcca accggcagac caatcacaaa gccaaaaaaa ccggatacga
    63721 aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc ggctcagaca
    63781 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
    63841 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc accggcgtaa
    63901 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
    63961 agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac gcagtcagtg
    64021 aatggttccg cttgcagacg ctcctgataa tcttccggga tatccagatg cagggtcgac
    64081 tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact taccgtgtcc
    64141 tgctcgatca gttcgccatt actgataacc gccacgcaat cacaaatgcg cttcacaacg
    64201 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttgat gtctttaagc
    64261 agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc atcacacagc
    64321 aatactttag gattgctggc taacgcacgg gcaatcgcca cacgctgttt ctgcccaccg
    64381 gaaaggttcg acggatagct atcatgctta tcaccaagac caaccaatga cagcaattcc
    64441 gtcacgcgac gtttgatttc gtctttcggc gtgttgtcca gctccagcgg cagagccacg
    64501 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
    64561 cggcgagctt tagtcaactc agattctgac agcgtggtca gctcctggcc atcaaccagc
    64621 acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt actcttaccc
    64681 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
    64741 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
    64801 atcattgatt atttattatc gtcattaagt tagtcgtggc atttcgaatg cctggaacgg
    64861 gcatcgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
    64921 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcacatg ccttattaag
    64981 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
    65041 aatgttgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
    65101 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac caaccagtct
    65161 ggcattgctc gcggtaaatt tactgaagca cagtttgaaa cgctgaccga gtggatggac
    65221 tggtcgctgg cggaccgcga tgtcgatctg gatggcatct attattgccc gcatcatccg
    65281 cagggtagtg ttgaagagtt tcgccaggtc tgcgactgcc gcaaaccaca tccggggatg
    65341 tttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
    65401 aaattagaag atatgcaggc agcggctgcg gcgagcgtgg gaacaaaagt gctggtgcgt
    65461 acgggtaaac ctattacgcc tgaagcagaa aacgcggcgg attgggtgtt aaatagtctg
    65521 gcagaccttc cgcaagcgat aaaaaagcag caaaaaccgg cgtgatgatt aaaagatgag
    65581 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga aacgactccc tataatgcgc
    65641 ctccatcgac acggcggatg tgaatcactt tacacaaaca gccggttcgg ttgaagagaa
    65701 aaatcctga
//
LOCUS       DAZFRE010000016        64061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFRE010000016 DAZFRE010000000
VERSION     DAZFRE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.016"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            296..631
                     /gene="yjdM"
                     /locus_tag="ACHKUI_001230"
     CDS             296..631
                     /gene="yjdM"
                     /locus_tag="ACHKUI_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6597471.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            791..1234
                     /gene="yjdN"
                     /locus_tag="ACHKUI_001231"
     CDS             791..1234
                     /gene="yjdN"
                     /locus_tag="ACHKUI_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6597472.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1367..2155
                     /gene="phnC"
                     /locus_tag="ACHKUI_001232"
     CDS             1367..2155
                     /gene="phnC"
                     /locus_tag="ACHKUI_001232"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6597473.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2180..3196
                     /gene="phnD"
                     /locus_tag="ACHKUI_001233"
     CDS             2180..3196
                     /gene="phnD"
                     /locus_tag="ACHKUI_001233"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6597474.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKYLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3302..4081
                     /gene="phnE"
                     /locus_tag="ACHKUI_001234"
     CDS             3302..4081
                     /gene="phnE"
                     /locus_tag="ACHKUI_001234"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6597475.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4102..4827
                     /gene="phnF"
                     /locus_tag="ACHKUI_001235"
     CDS             4102..4827
                     /gene="phnF"
                     /locus_tag="ACHKUI_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6597476.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEHQLAVR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ENSPAEYSVSLTRADMIELTMEH"
     gene            4828..5280
                     /gene="phnG"
                     /locus_tag="ACHKUI_001236"
     CDS             4828..5280
                     /gene="phnG"
                     /locus_tag="ACHKUI_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6597477.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5277..5861
                     /gene="phnH"
                     /locus_tag="ACHKUI_001237"
     CDS             5277..5861
                     /gene="phnH"
                     /locus_tag="ACHKUI_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6597478.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5861..6925
                     /gene="phnI"
                     /locus_tag="ACHKUI_001238"
     CDS             5861..6925
                     /gene="phnI"
                     /locus_tag="ACHKUI_001238"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6597479.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGDQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQLPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6918..7763
                     /gene="phnJ"
                     /locus_tag="ACHKUI_001239"
     CDS             6918..7763
                     /gene="phnJ"
                     /locus_tag="ACHKUI_001239"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6597480.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRHFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGKRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7760..8518
                     /gene="phnK"
                     /locus_tag="ACHKUI_001240"
     CDS             7760..8518
                     /gene="phnK"
                     /locus_tag="ACHKUI_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6597481.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8629..9309
                     /gene="phnL"
                     /locus_tag="ACHKUI_001241"
     CDS             8629..9309
                     /gene="phnL"
                     /locus_tag="ACHKUI_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6597482.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9306..10442
                     /gene="phnM"
                     /locus_tag="ACHKUI_001242"
     CDS             9306..10442
                     /gene="phnM"
                     /locus_tag="ACHKUI_001242"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6597483.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10442..10999
                     /gene="phnN"
                     /locus_tag="ACHKUI_001243"
     CDS             10442..10999
                     /gene="phnN"
                     /locus_tag="ACHKUI_001243"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6597484.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLSLIHQKEKHHACL"
     gene            10986..11420
                     /gene="phnO"
                     /locus_tag="ACHKUI_001244"
     CDS             10986..11420
                     /gene="phnO"
                     /locus_tag="ACHKUI_001244"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO6597485.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11422..12180
                     /gene="phnP"
                     /locus_tag="ACHKUI_001245"
     CDS             11422..12180
                     /gene="phnP"
                     /locus_tag="ACHKUI_001245"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6597486.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHINHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12215..12544)
                     /gene="yjdP"
                     /locus_tag="ACHKUI_001246"
     CDS             complement(12215..12544)
                     /gene="yjdP"
                     /locus_tag="ACHKUI_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6597487.1"
                     /translation="MKRFPLFLLFTLFTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12613..13062)
                     /gene="rpiB"
                     /locus_tag="ACHKUI_001247"
                     /gene_synonym="alsI"
     CDS             complement(12613..13062)
                     /gene="rpiB"
                     /locus_tag="ACHKUI_001247"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO6597488.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13421..14311
                     /gene="alsR"
                     /locus_tag="ACHKUI_001248"
     CDS             13421..14311
                     /gene="alsR"
                     /locus_tag="ACHKUI_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO6597489.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14370..15305
                     /gene="alsB"
                     /locus_tag="ACHKUI_001249"
     CDS             14370..15305
                     /gene="alsB"
                     /locus_tag="ACHKUI_001249"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO6597490.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15432..16964
                     /gene="alsA"
                     /locus_tag="ACHKUI_001250"
     CDS             15432..16964
                     /gene="alsA"
                     /locus_tag="ACHKUI_001250"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO6597491.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            16943..17923
                     /gene="alsC"
                     /locus_tag="ACHKUI_001251"
     CDS             16943..17923
                     /gene="alsC"
                     /locus_tag="ACHKUI_001251"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO6597492.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17934..18629
                     /gene="alsE"
                     /locus_tag="ACHKUI_001252"
     CDS             17934..18629
                     /gene="alsE"
                     /locus_tag="ACHKUI_001252"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO6597493.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18613..19542
                     /gene="alsK"
                     /locus_tag="ACHKUI_001253"
     CDS             18613..19542
                     /gene="alsK"
                     /locus_tag="ACHKUI_001253"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO6597494.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19815..21800
                     /gene="yjcS"
                     /locus_tag="ACHKUI_001254"
     CDS             19815..21800
                     /gene="yjcS"
                     /locus_tag="ACHKUI_001254"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6597495.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIIAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHTVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            22009..22284
                     /gene="ytcA"
                     /locus_tag="ACHKUI_001255"
     CDS             22009..22284
                     /gene="ytcA"
                     /locus_tag="ACHKUI_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6597496.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            22303..23334
                     /gene="mdtN"
                     /locus_tag="ACHKUI_001256"
     CDS             22303..23334
                     /gene="mdtN"
                     /locus_tag="ACHKUI_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6597497.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASSVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            23334..25385
                     /gene="mdtO"
                     /locus_tag="ACHKUI_001257"
     CDS             23334..25385
                     /gene="mdtO"
                     /locus_tag="ACHKUI_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6597498.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     VSEINKLQHTIAEGQCWQSDWRLSEDEAVAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRFPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            25382..26848
                     /gene="mdtP"
                     /locus_tag="ACHKUI_001258"
     CDS             25382..26848
                     /gene="mdtP"
                     /locus_tag="ACHKUI_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6597499.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDASALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQTAPVVEKK"
     gene            27046..29193
                     /gene="fdhF"
                     /locus_tag="ACHKUI_001259"
     CDS             27046..29193
                     /gene="fdhF"
                     /locus_tag="ACHKUI_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:27463..27465,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO6597500.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            29568..31136
                     /locus_tag="ACHKUI_001260"
     CDS             29568..31136
                     /locus_tag="ACHKUI_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6597501.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            31236..32285
                     /locus_tag="ACHKUI_001261"
     CDS             31236..32285
                     /locus_tag="ACHKUI_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6597502.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            32285..33118
                     /locus_tag="ACHKUI_001262"
     CDS             32285..33118
                     /locus_tag="ACHKUI_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6597503.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMTADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            33111..33947
                     /locus_tag="ACHKUI_001263"
     CDS             33111..33947
                     /locus_tag="ACHKUI_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6597504.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVIQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            33934..34638
                     /locus_tag="ACHKUI_001264"
     CDS             33934..34638
                     /locus_tag="ACHKUI_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6597505.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            34749..35438
                     /gene="yjcO"
                     /locus_tag="ACHKUI_001265"
     CDS             34749..35438
                     /gene="yjcO"
                     /locus_tag="ACHKUI_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6597506.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(36039..37352)
                     /gene="gltP"
                     /locus_tag="ACHKUI_001266"
     CDS             complement(36039..37352)
                     /gene="gltP"
                     /locus_tag="ACHKUI_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6597507.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(37694..38290)
                     /gene="nrfG"
                     /locus_tag="ACHKUI_001267"
     CDS             complement(37694..38290)
                     /gene="nrfG"
                     /locus_tag="ACHKUI_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6597508.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSKQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(38287..38670)
                     /gene="nrfF"
                     /locus_tag="ACHKUI_001268"
     CDS             complement(38287..38670)
                     /gene="nrfF"
                     /locus_tag="ACHKUI_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6597509.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(38663..40381)
                     /gene="nrfE"
                     /locus_tag="ACHKUI_001269"
     CDS             complement(38663..40381)
                     /gene="nrfE"
                     /locus_tag="ACHKUI_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6597510.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSVLFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWR
                     GRKRDE"
     gene            complement(40401..41357)
                     /gene="nrfD"
                     /locus_tag="ACHKUI_001270"
     CDS             complement(40401..41357)
                     /gene="nrfD"
                     /locus_tag="ACHKUI_001270"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6597511.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(41354..42025)
                     /gene="nrfC"
                     /locus_tag="ACHKUI_001271"
     CDS             complement(41354..42025)
                     /gene="nrfC"
                     /locus_tag="ACHKUI_001271"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6597512.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(42022..42588)
                     /gene="nrfB"
                     /locus_tag="ACHKUI_001272"
     CDS             complement(42022..42588)
                     /gene="nrfB"
                     /locus_tag="ACHKUI_001272"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6597513.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(42633..44069)
                     /gene="nrfA"
                     /locus_tag="ACHKUI_001273"
     CDS             complement(42633..44069)
                     /gene="nrfA"
                     /locus_tag="ACHKUI_001273"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6597514.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAMTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            44461..46419
                     /gene="acs"
                     /locus_tag="ACHKUI_001274"
     CDS             44461..46419
                     /gene="acs"
                     /locus_tag="ACHKUI_001274"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6597515.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGHPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            46710..47024
                     /gene="yjcH"
                     /locus_tag="ACHKUI_001275"
     CDS             46710..47024
                     /gene="yjcH"
                     /locus_tag="ACHKUI_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6597516.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            47021..48670
                     /gene="actP"
                     /locus_tag="ACHKUI_001276"
     CDS             47021..48670
                     /gene="actP"
                     /locus_tag="ACHKUI_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6597517.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(48709..49398)
                     /locus_tag="ACHKUI_001277"
     CDS             complement(48709..49398)
                     /locus_tag="ACHKUI_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFO6597518.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDMLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(49391..49801)
                     /locus_tag="ACHKUI_001278"
     CDS             complement(49391..49801)
                     /locus_tag="ACHKUI_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6597519.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            49905..50789
                     /locus_tag="ACHKUI_001279"
     CDS             49905..50789
                     /locus_tag="ACHKUI_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6597520.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(50825..52474)
                     /gene="yjcE"
                     /locus_tag="ACHKUI_001280"
     CDS             complement(50825..52474)
                     /gene="yjcE"
                     /locus_tag="ACHKUI_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6597521.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(52625..53974)
                     /gene="ghxP"
                     /locus_tag="ACHKUI_001281"
                     /gene_synonym="yjcD"
     CDS             complement(52625..53974)
                     /gene="ghxP"
                     /locus_tag="ACHKUI_001281"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6597522.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(54520..54984)
                     /gene="soxR"
                     /locus_tag="ACHKUI_001282"
     CDS             complement(54520..54984)
                     /gene="soxR"
                     /locus_tag="ACHKUI_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6597523.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            55070..55393
                     /gene="soxS"
                     /locus_tag="ACHKUI_001283"
     CDS             55070..55393
                     /gene="soxS"
                     /locus_tag="ACHKUI_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6597524.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(55429..56982)
                     /gene="pdeC"
                     /locus_tag="ACHKUI_001284"
     CDS             complement(55429..56982)
                     /gene="pdeC"
                     /locus_tag="ACHKUI_001284"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6597525.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNYLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKNQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            57411..57692
                     /gene="yjcB"
                     /locus_tag="ACHKUI_001285"
     CDS             57411..57692
                     /gene="yjcB"
                     /locus_tag="ACHKUI_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6597526.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(57791..58327)
                     /gene="ssb1"
                     /locus_tag="ACHKUI_001286"
     CDS             complement(57791..58327)
                     /gene="ssb1"
                     /locus_tag="ACHKUI_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6597527.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            58582..61404
                     /gene="uvrA"
                     /locus_tag="ACHKUI_001287"
     CDS             58582..61404
                     /gene="uvrA"
                     /locus_tag="ACHKUI_001287"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6597528.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSTNVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(61613..61969)
                     /gene="yjbR"
                     /locus_tag="ACHKUI_001288"
     CDS             complement(61613..61969)
                     /gene="yjbR"
                     /locus_tag="ACHKUI_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6597529.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(61973..62389)
                     /gene="yjbQ"
                     /locus_tag="ACHKUI_001289"
     CDS             complement(61973..62389)
                     /gene="yjbQ"
                     /locus_tag="ACHKUI_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6597530.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(62500..63213)
                     /gene="aphA"
                     /locus_tag="ACHKUI_001290"
     CDS             complement(62500..63213)
                     /gene="aphA"
                     /locus_tag="ACHKUI_001290"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6597531.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            63490..>64061
                     /locus_tag="ACHKUI_001291"
     CDS             63490..>64061
                     /locus_tag="ACHKUI_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HFO6597532.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKA"
BASE COUNT        15627 a        17319 c        16616 g        14499 t
ORIGIN      
        1 gcgtgttgtt ttttattaga ataaataaca tcttccaata cgtttaaaac gtcagaaaga
       61 taaaaacatc atgtgaatta aaaaaagaac gagtagagca ttaaccttat cttaaataat
      121 aaatggaggc aaaacgatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtca
      181 tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt
      241 cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc
      301 attaccacac tgcccaaaat gcaactccga atacacctac gaagataacg gcatgtacat
      361 ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct
      421 gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa
      481 agatctgaaa gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag taaaaaacat
      541 ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa
      601 actgaagtct gagtttgtga aaaagaactg attgtattgt gatcggtagg ccggataagg
      661 cgctcgcgcc gcatccggca acggtggcga ctgcctgatg cgacgctgac cgcgtcttat
      721 caggcttccc tctcatctgt ataaatttcg aactacactt aacgggcttc tcttaactga
      781 ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt ccgacgcgat
      841 tgcctattat caacgtacgt tgggcgcgga actgctctat aaaatcagct tcggcgaaat
      901 gccaaaatca acgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat ttcccgatac
      961 cgccatcgcc cacgccaatg tgcgcattgc aggaagcgac atcatgatga gcgatgctat
     1021 accgtcagga aaagccagct actccggctt tacgctggtg ctcgattctc aacaggtgga
     1081 agaaggaaaa cgctggtttg ataaccttgc cgccaacgga aaaatcgaaa tggcctggca
     1141 ggaaacgttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg taccgtggat
     1201 gattaatgtc atcaaacaac agccaacgca ataacccgcc gggaggcccg ccctcccgca
     1261 ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa ccaaatcgtc
     1321 acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc aaacgattat
     1381 ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcag gcgctgcacg cggttgatct
     1441 gaacattcat catggtgaaa tggtggctct gcttgggccg tcgggttccg gaaaatccac
     1501 tcttttacgt cacttaagcg gtttgattac cggcgataaa tctgtcggta gccatatcga
     1561 gctgctgggc cgcactgtcc agcgcgaagg ccgtctggcc cgcgatatcc gcaaaagccg
     1621 cgcccatacc ggctacatct ttcaacaatt caacctggtg aatcgcctga gcgtactgga
     1681 gaacgtgctg attggcgcgc tcggcagcac gccattctgg cgaacctgtt ttagctactt
     1741 cacccgcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca tggtgcactt
     1801 tgcccatcaa cgcgtttcca cactctccgg cggccagcaa cagcgtgtgg cgattgcccg
     1861 cgcgctgatg cagcaggcga aggtcattct cgctgacgaa cccatcgcct cgctggaccc
     1921 ggaatcggca cgcatcgtga tggacaccct gcgcgacatc aaccagaacg acggcattac
     1981 cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac gcatcgtcgc
     2041 cctgcgccag gggcacgtct tctacgacgg ctgcagccaa cagtttgata acgaacgttt
     2101 tgaccatctc taccgcagca ttaaccgcgt cgaagagaac gcgaaagctg cctgacatcc
     2161 ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct tcaccagcat
     2221 gttcagcctc agcaccctgt taaatccggc atacgccgaa gagcaggaaa aggcgctgaa
     2281 tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga cgccgttctt
     2341 acaggatatg gagaagaagc tgggcgtaaa ggtcaacgcc ttctttgccc cggactacgc
     2401 gggcattatc caggggatgc gcttcaataa agtggatatc gcctggtacg gcaacctgtc
     2461 ggcgatggaa gcggtggatc gcgccaacgg tcaggtcttc gcccagacgg tcgcggcgga
     2521 tggatcgccg ggttactgga gcgtgttgat cgtcaacaaa gatagcccga tcaacaacct
     2581 gaacgatctg ctggcgaagc ggaaatatct caccttcggc aatggcgatc ctaactctac
     2641 ctccggcttc ctcgtccccg gttactacgt cttcgctaaa aacaatatct ccgccagcga
     2701 ctttaagcgc accgtcaacg ccgggcatga aaccaacgcc ctcgccgtcg ctaacaagca
     2761 agtggatgtc gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct ccgcgccgga
     2821 gaagctgaaa gaactgaagg tgatctggaa atcaccgctg atccctggcg atccgatcgt
     2881 ctggcgcaaa aatctttccg aaaccactaa agacaagatc tacgacttct ttatgaatta
     2941 cggcaaaacg ccggaagaga aagcggtgct ggagcgcctg ggctgggcgc cgttccgcgc
     3001 ctccagcgac ttgcaactgg tgccgattcg ccagctcgca ctgtttaaag agatgcaaag
     3061 cgtgaaggac aacaaagggc tgaacgaaca ggacaagctg gcaaaaacca ccgccattca
     3121 ggcgcaactg gatgatttgg accgcctgaa caacgcgtta agcgcaatga gttcggtgag
     3181 taaagcggtg cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt
     3241 gcagacgttg atgccggatg cggtgcaagc accttatccg gcctacagac cggagccaaa
     3301 catgcaaacc atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc tgagctgggc
     3361 cgttgttatc gccgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc cgcttacgct
     3421 gattaaagat ggcggcaaca tggcgacgtt cgccgccgac ttcttcccgc ccgatttcag
     3481 ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg tctggggcac
     3541 cgcgctggcg gtggttctct ccatcccctt cggcctgatg agcgctgaaa acctggtacc
     3601 gtggtgggtc taccaacctg tacgccgcct gatggacgcc tgccgcgcca ttaacgaaat
     3661 ggtcttcgcc atgctgttcg tggtcgccgt cggcctcggc ccgttcgctg gcgtgctggc
     3721 gctgtttatc cacaccaccg gcgtactttc caaactgctt tccgaagcgg tagaagcgat
     3781 tgaacccggc ccggtggagg gcattcgcgc taccggagcc aacaagctgg aagagatcct
     3841 ctacggcgta ctaccgcagg tcatgccgct gctgatttcc tactccctct atcgcttcga
     3901 atccaacgtc cgctcggcga ccgtcgtcgg tatggttggc gcgggcggga tcggcgtcac
     3961 gctgtgggaa gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga tggtgctgat
     4021 tatcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc actttatctg
     4081 attagcgagg cattgatggc tatgcacttg tctacacatc cgaccagcta cccaacacgc
     4141 tatcaggaga tagccgcaaa acttgagcag gagcttcgtc agcattaccg ctgtggcgat
     4201 tatcttcccg ccgaacacca actggcggtg cgctttgagg tgaaccgcca caccctgcgc
     4261 cgcgccatcg accaactggt ggaaaaaggc tgggtacagc gccgtcaggg cgtcggcgtg
     4321 ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt tagccagaac
     4381 ctgctggacc agggcagcca tcccaccagc gaaaaactgc tgtcggtact gcgccccgcc
     4441 tccggtcacg tcgctgacgc gctggggatc gccgagggcg agaacgttat ccacctgcgc
     4501 accctgcgcc gggtcaacgg cgttgcgctc tgtttaatcg accactactt cgcagatctc
     4561 acgctctggc caacgcttca atgctttgac agcggttcgc tgcacgattt tctgcgcgag
     4621 caaacgggga ttacgctacg ccgcagccag acgcggatca gcgcccgccg cgcccaggcc
     4681 aaagagtgcc agcgtcttga aatcccgaat atgtcgccgc tgctgtgcgt gcgcaccctt
     4741 aaccaccgtg acggtgaaaa cagtccggcg gagtactccg tcagcctgac gcgcgccgac
     4801 atgattgaac tcactatgga gcactgaatg cacgcagata ccgcgacccg ccagcactgg
     4861 atgtccgtgc tggcgcacag ccaaccggct gaactggcag cccgcctgaa cgcgctgaac
     4921 atcaacgccg attatgaggt gatccgcgcc gctgaaactg gcctggtgca gattcaggcg
     4981 cgaatgggcg gcaccggcga acgctttttt gctggcgacg ccacgctcac ccgcgccgcc
     5041 gtgcgtctga ctgacggcac gctcggctat agctgggtgc tggggcgcga caaacagcac
     5101 gccgaacgtt gtgcgctgat tgatgcgctg atgcagcaaa accgccactt tcaaaactta
     5161 tcagaaaccc ttatcgcccc gctggacgct gaccgtatgg cacgcattgc cgcgcgtcag
     5221 gccgaagtga acgccagccg ggtcgacttc tttacgatgg ttcgcggaga caacgcatga
     5281 ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt cgtcgcctgt
     5341 taaaggccat gagcgagccg ggcgtgattg tcgccctgca tcagctcaag cgcggctggc
     5401 agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggccgataac gacacgccgg
     5461 tgtggcttgc cgcgccattg agcaacgata tcgtcaatca gagcctgcgt tttcatacca
     5521 acgcgtcgct ggttaatcag ccggaacagg cgaccttcgc ggtaacagat gaggcgatct
     5581 ccagcgagca gctcaacgcc ctttccaccg gcactgccgt tgcaccagaa gcgggcgcaa
     5641 cgctgatttt acaggtcacc agcctgagcg gcgggcgtat gttgcgcctc accggcgcag
     5701 gtattgccga agaacggatg atcgccccgc aactgccaaa gtgcattctt catgaactta
     5761 ccgagcgccc gcacccgttc ccgctcggca tcgacctgat cctgacctgc ggcgagcgcc
     5821 tgctggctat tccgcgaacc actcatgtgg aggtgtgctg atgtacgttg ccgtgaaagg
     5881 gggcgaaaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc ggggcgatac
     5941 tgatttgcct gaactgagcg tcgcccagat tgaacagcag cttaacctcg cggtagatcg
     6001 cgtgatgacc gaaggcggta ttgccgaccg cgagctggcc gcgctggcgc tgaaacaggc
     6061 cagcggcgat aacgtcgaag cgattttcct gttgcgcgcc taccgcacca cgctggcgaa
     6121 gctggcggtc agtgagccgc tcgacaccac cgggatgcgt ctcgaacggc gtatttcagc
     6181 aatttataaa gacattcccg gcgaccaact gcttggcccc acctacgact acacccatcg
     6241 cctgctcgat ttcaccctgc tggcaaacgg cgaaacgccg acgctgacca cagccgacag
     6301 cgaacaactg ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc tggcgaagtt
     6361 tgaagaagat agcggcgcac agccggacga catcacccgc acgccgccgg tttacccctg
     6421 ctcacgctcc tcccgcctgc aacaattaat gcgcggcgac gaaggctatt tgctggcgct
     6481 ggcctactcc acccagcgtg gttacggacg caatcatccg ttcgcaggcg aaatccgcag
     6541 cggctatatc gacgtgtcga ttgtgccgga agagctggga tttgcggtga acgtcggcga
     6601 actgctgatg accgaatgtg aaatggtcaa cggttttatc gacccgccgg atgagccgcc
     6661 gcacttcacg cgcggctacg ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat
     6721 ggcgctggtt gaccgcgctc tacaagcccc ggagtacggc gagcacgcga caggcccggc
     6781 gcaggatgaa gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag gctttgtctc
     6841 gcacctcaaa ctcccgcact acgtcgattt ccaggccgaa ctggagctac tcaaacgtct
     6901 gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac ctcgacgagc
     6961 agaccaaacg catgatccgc cgcgccatct taaaagcggt ggcaatcccc ggttatcagg
     7021 tgccgtttgg cggacgcgag atgccgatgc cctacggctg gggaaccggc ggcattcagc
     7081 tcaccgccag cgtgattggc gaaagcgacg tgttgaaggt gattgaccag ggcgcagacg
     7141 acaccaccaa cgccgtgtcg attcgtcact tctttaagcg cgtgaccggg gtgaacacca
     7201 ccgagcgcac ggacgatgcg acacttatcc agacgcgtca ccgcataccc gaaacgccgc
     7261 tgaccgaaga tcagatagtt atcttccagg tgccgatccc cgaaccgctg cgctttattg
     7321 agccgcgtga gacggaaacg cgcaccatgc acgcgctgga ggagtacggc gtgatgcagg
     7381 tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc caccacctac gcctatccgg
     7441 tgaaggtgaa cgggcgctac gtaatggacc cgtcgccgat cccgaaattc gataacccga
     7501 aaatggacat gatgcccgcc ctgcaactgt ttggcgcggg gcgcgagaag cgcatctatg
     7561 ccgtgccacc gtttacccgc gtggaaagcc tcgatttcga tgatcatccg ttcaccgtcc
     7621 agcagtggga tgaaccatgc gccatctgcg gatcgaccca cagctatctt gatgaagtgg
     7681 tgctggatga cgccggaaaa cgcatgtttg tctgctccga taccgattat tgccgccaac
     7741 agaacgaggc caaaagccaa tgaatcaacc gttactttcg gtcaataacc tgacccacct
     7801 ttatgccccg ggcaaaggct tcagcgatgt ctcttttgat ttatggccag gagaagttct
     7861 gggcattgtt ggggagtccg gttccgggaa gaccacgcta ctgaaatcaa tttccgcccg
     7921 cctgacacca cagcaagggg aaattcgcta cgagaaccgt tcgctgtacg cgatgagcga
     7981 ggccgaccgc cgtcgcctgc tgcgcaccga atggggtgtg gtgcatcagc atccgcttga
     8041 cggcctgcgc cgccaggtgt cggcaggcgg caatatcggc gaacggttga tggcaaccgg
     8101 ggcgcgtcat tacggcgata ttcgtgccac cgcgcagaag tggctggaag aggtggagat
     8161 tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggtatgc agcagcgttt
     8221 gcagattgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggacg agccaaccgg
     8281 cgggctggat gtatcggtac aggcgcgcct gctcgatctg ctgcgcggtc tggtggtgga
     8341 gctgaacctg gcggtagtga ttgttaccca cgatctgggc gtcgcccgcc tgctggcgga
     8401 ccgtttgctg gtgatgaaac aggggcaagt ggtggagagt gggttaaccg accgcgtgct
     8461 cgacgaccct caccatccat atacccagtt gctggtgtca tcggttttgc agaattgagc
     8521 tgatgccgga tgcggcgtaa acgccttatc cggcctacaa atgcgctccc cgtaggtcgg
     8581 ataagacgca tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat gattaacgta
     8641 caaaacgtca gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct gcctgtatta
     8701 caaggcgcct cactcaccgt caacgccggc gaatgcgtgg tactccacgg ccattccggc
     8761 agtggaaaat caactctgct acgctcgctg tatgccaact atctacccga cgaagggcaa
     8821 atccagatca aacacggtga tgaatgggtc gacctggtca ccgcgccagc gcgcaaagtg
     8881 gtggagatcc gcaaaaccac tgtcggctgg gtaagccagt ttctgcgcgt catcccacgt
     8941 atttcggcac tggaagtggt gatgcagccg ctgctcgata ccggcgtacc gcgtgaagcc
     9001 tgcgccgcta aagccgcgcg tctcctcacc cgcctgaacg tgcctgaacg cctgtggcac
     9061 ctcgcgccat cgaccttctc cggtggcgaa cagcagcgtg tcaacatcgc ccgtggcttt
     9121 atcgtcgact accccattct gctgctcgac gaacctaccg cctcgctgga cgctaaaaac
     9181 agcgccgccg tagtggaact gatccgcgaa gccaaagccc gcggcgcggc catcgtcggt
     9241 atcttccatg acgaaaccgt gcgcgagcaa gtcgccgacc gcctgcaccc aatgggagtc
     9301 tcttcatgat tatcaataac gtcaaactgg tgctggaaaa cgaggtggta cacggttctc
     9361 tggagatgca ggacggcgaa atccgcgcct ttgccgaaag ccagagtcac ctgtcagaag
     9421 caatggacgg cgaaggcggc tggctgctac cggggctgat tgagctgcat accgataatc
     9481 tggataaatt cttcaccccg cgcccgaaag ttgactggcc tgcccactcg gcgatgagca
     9541 gccacgacgc gctgatggtg gcgagcggca tcaccaccgt gctggacgcc gtggcgattg
     9601 gtgatgtacg cgacggcggc gatcggctgg agaatctgga gaagatgatc aacgccatcg
     9661 aagagacgca gaaacgcggc gtcaaccgtg ccgagcaccg tctgcaccta cgctgcgaac
     9721 tgccgcatca caccacgctg ccgctgtttg aaaaactggt acagcgcgaa ccggtaacgc
     9781 tggtgtcgct gatggatcac tcaccgggcc agcgccagtt cgccaaccgc gaaaaatatc
     9841 gtgaatatta tcagggcaaa tactcgctca ccgatgcgca gatgcaacag tacgaagagg
     9901 agcaactggc gctcgccgca cgctggtcac agccgaatcg cgaaactatc gccgcaatgt
     9961 gccgcgcgcg acacattgcg ctcgccagcc acgatgacgc cacgcacgcc cacgtagccc
    10021 aatcccatca gcttggcagc gtgatcgccg aatttcccac cacgttcgaa gcggcagaag
    10081 cctcgcgcaa gcatggcatg aacgtgctga tgggcgcgcc gaatatcgtg cgcggcggtt
    10141 cgcactccgg caacgtggcg gccagtgaac tggcgcagct tggcctgctg gatatcctct
    10201 cttccgacta ctaccccgcc agcctgctcg atgcggcatt tcgcgtcgcc gatgacgaga
    10261 gcaaccgctt tacgctgccg caggcggtga agctggtgac taaaaatccg gcgcaggcgc
    10321 ttaatcttca ggatcgcggg gtgattggcg agggcaaacg cgccgacctg gtgctggcgc
    10381 atcgcaaggg caaccacatt catatcgacc acgtctggcg tcagggtaaa agggtgttct
    10441 gatgacggga aaactgattt ggttaatggg ggcctctggc tccggaaaag acagtctgtt
    10501 gacggaactc cgccagcggg aacaaactca gctactggta gcgcatcgct acatcacgcg
    10561 cgccgccagc gccggaagtg aaaaccatat cgccctgagc gagcaggagt tttttacccg
    10621 cgcggggcag aaccttctgg ccttaagctg gcacgctaac ggtctgtatt atggcgtcgg
    10681 cgtcgagatc gacctctggc tgcacgctgg atttgacgtg gtggtcaacg gctcacgcgc
    10741 ccacctgccg caggcgcggg cgcgttatca atcggcgctg ctgcccatct gtttacaggt
    10801 ttcgccggag atcctgcgcc agcgcctgga aaaccgtggc cgcgaaaacg ccagtgaaat
    10861 taatgcccgg ctggcgcgcg ccgcccgcta tactcctcag gattgccata cactcaataa
    10921 tgacggcagc ctgcgccagt cggtcgacac gctgctgtcg ctgattcatc agaaggagaa
    10981 acaccatgcc tgcttgtgaa cttcgcccgg caacgcagta cgacaccgac gcggtttacg
    11041 cgctgatttg tgagctaaaa caagcggagt ttgaccacca ggcgtttcgc gtgggcttta
    11101 acgccaacct gcgtgacccg aatatgcgct accatctggc gctgttggat ggcgaggttg
    11161 tcggcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa
    11221 ttcaggaact ggtggtgatg ccgcaggcgc gcggtctgaa catcggcagt aaattactgg
    11281 catgggcaga agaagaagcc cgccaggccg gggcagaaat gaccgagctt tccaccaacg
    11341 tcaagcgcca cgacgcgcac cgtttctatc tgcgcgaagg ctacgagcag agccacttcc
    11401 gcttcaccaa ggcgctgtaa catgagcctg accctcacgc tcaccggcac cggcggcgca
    11461 cagggcgttc cggcatgggg ctgcgagtgc gcggcctgcg ccagagcacg acgctcgccg
    11521 cagtatcgcc gccagccatg cagcggcgta gtaaagttta acgacgcaat caccctgatc
    11581 gacgccgggc tgcacgatct cgccgatcgc tggtcgccag gatcgttcca gcagtttttg
    11641 ctgacgcatt atcatatgga tcacgtccag gggctgtttc cgctgcgctg gggcgtgggc
    11701 gatcagatcc cggtctacgg cccgccggat gaacagggct gcgacgatct gtttaaacat
    11761 ccgggcctgc ttgatttcag ccacacgatg gaaccgtttg tggtgtttga tttacagggg
    11821 ttacaggtca cgcccctgcc gctcaaccac tcaaaactga ccttcggtta tctgctggaa
    11881 acggcacaca gccgggtggc gtggctgtct gacaccgctg gcttgccgga aaaaacgctg
    11941 aaatttttac gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc
    12001 gaggatgcac cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc
    12061 cgctcgccac gggtgattct gacccatatc aaccaccagt ttgatgcgtg gctgatggaa
    12121 aacgcactgc cgtcggggtt tgaggcgggg tttgatggga tggagattgg ggtggcgtga
    12181 tgggtgaggg gcgatgcctg ctctaaactc aacatcaacg cccatactca tcttccattc
    12241 tccgctcttc atcttccagt tgccgacgct cctgatccag ctggcgctgg cgatcgtcca
    12301 gctgcctgcg gcgatcttca aactggcggc ggcggtcatc atattgtctg cgccgatcgt
    12361 cgttcacttc acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag
    12421 ggttataagc gtcgttaatc gcctgctgaa tattgccaat ggtgtcatcg ataatatcgg
    12481 cctgggccgg aacggtggac agcgtgaaca gggtgaataa aagaaatagc ggaaagcgtt
    12541 tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt
    12601 agtggatgaa tctcaatttc tccgctgctc tattgccgta atcgcctcca cgcgttgttg
    12661 atgacgaccg ccttcgtact gtgcgcccag ccacgcatcg acaatcattt ttgccagttc
    12721 gaggccaact actcgtgaac caaaagccag cacgttggtg tcgttatgct gccgcgaaag
    12781 ttgcgcggaa taaggttcgc tacaaacgac cgcgcgaatt ccggcaaact tgttcgccgc
    12841 tatcgaaata ccgacgccag tgccacaaat caaaatcccg ccatcagcct ctccgccagc
    12901 aacggccagt gcgacttgac tggcgtaatg cggataatca gtacgctctg acgaccaggt
    12961 tcctttatca atcacttcaa cgccacgctc aactaaatgt gccactattt catgttttaa
    13021 aatgaaaccg acatgatcac agccaaatgc aatctttttc attcatcttc cccgcccgtg
    13081 caaaacttca caatcaaaaa acatttgtga tgtaaatgaa ttaaaaacac ccaaaatcga
    13141 tgaaatccgc cttcccgatc tgttttacag agttacatct ggcagcgcaa tagcgttaaa
    13201 gacactcact atccagcaac atctatcatc caataaacca gaaaaacaaa taacatcatg
    13261 tttttaaagt gattaaatga aatgaaattt taagccactc tccattattc acaacaaaac
    13321 aaactttata aattttattt ttttgtgaag ttgccagcat cttttctgtt cttgctgtgg
    13381 tgatatagtg gcgtcttcaa ttcaaggaca agagaacgtg atgagccagt cagagtttga
    13441 ttcagcgctt ccgaacggta tcgggttagc gccttacctg cgaatgaagc aggaaggaat
    13501 gacagaaaac gaaagccgca tcgtggagtg gttactcaaa cccggtaacc tgagttgtgc
    13561 acccgcaatt aaagatgtcg cagaagctct ggcggtatct gaagcgatga tcgttaaggt
    13621 atcaaagctg ctggggttta gcggctttcg taacttacgc agtgcgctgg aagattattt
    13681 ttctcagtca gaacaggtat tgccgtccga gttggctttt gatgaagcgc cgcaggatgt
    13741 ggtgaataag gtatttaaca tcactttacg caccattatg gaaggtcagt cgatcgtcaa
    13801 cgttgatgag atccaccgtg ccgcccgctt tttctatcag gccagacagc gggatttgta
    13861 cggtgccgga ggatcaaatg ctatctgtgc tgatgtacag cacaagtttt tacgcattgg
    13921 cgtacgctgc caggcctatc cggacgctca catcatgatg atgtctgctt cgttgttaca
    13981 ggaaggagat gtggtgctgg tggtgactca ttccgggcga accagtgatg taaaagcggc
    14041 tgtagaactg gcaaaaaaga acggggcaaa gattatttgt ataacgcata gctatcattc
    14101 accaatagcg aagctggccg attatattat ttgctcaccg gccccggaaa caccgttatt
    14161 aggtcgtaat gcctcggcaa gaatattaca actgactttg ctggacgcgt tttttgtctc
    14221 tgtcgcccag ctcaacattg aacaagctaa tattaatatg caaaaaaccg gcgcaattgt
    14281 tgatttcttc tcaccaggcg cgctgaaata agaaaaaggt aattccgcca tcgcggagtt
    14341 acctctcccc ttaccgtgag aacatgatta tgaataaata tctgaaatat ttcagcggca
    14401 cactcgtggg cttaatgttg tcaaccagcg cttttgctgc cgccgaatat gctgtcgtat
    14461 tgaaaacact ctccaaccca ttttgggtag atatgaaaaa aggcattgaa gatgaagcaa
    14521 aaacgctggg cgtcagcgtt gatatttttg cctctccttc agaaggcgat tttcaatctc
    14581 aattgcagtt atttgaagat ctcagtaata aaaattacaa aggtatcgcc ttcgcgccat
    14641 tatcctcagt gaatctggtc atgcctgtcg cccgcgcatg gaaaaaaggc atttatctgg
    14701 ttaatctcga tgaaaaaatc gacatggata atctgaaaaa agctggcggc aatgtggaag
    14761 gttttgtcac caccgataac gttgctgtcg gggcgaaagg cgcgtcgttc attattgata
    14821 aattgggcgc tgaaggtggt gaagtcgcaa tcattgaggg taaagccggt aacgcctccg
    14881 gtgaagcgcg tcgtaatggt gccaccgaag ccttcaaaaa agcaagccag atcaagcttg
    14941 tcgccagcca gcctgccgac tgggaccgca ttaaagcact ggatgtcgcc actaacgtgt
    15001 tgcaacgtaa tccgaatatt aaagcgatct attgcgcgaa tgacacgatg gcaatgggtg
    15061 ttgctcaggc agtcgcaaac gccggaaaaa cgggcaaagt gttggtcgtc ggtacagatg
    15121 gaattccgga agcccgcaaa atggtggaag ccggacaaat gaccgcgacg gttgcccaga
    15181 acccggcgga tatcggtgca acgggtctga agctgatggt tgacgctgag aaatccggca
    15241 aggttatccc gctggataaa gcaccggaat ttaaactggt cgattcaatc ctggtcactc
    15301 aataaaactg aatgatgccg gatgatgatg ctaatgaagt gtcttatccg gccaacaaaa
    15361 taacctgaac gtagacctga taaacgtagc catcaggcaa taatacgata tttcgtgcag
    15421 agaggttatt tatggccacg ccatatatat cgatggcggg gatcggcaag tcctttggtc
    15481 cggttcacgc attaaagtcg gttaatttaa cggtttatcc tggtgaaata catgcattac
    15541 taggagaaaa tggtgcgggt aaatctacgc taatgaaagt gttatccggg atacatgagc
    15601 cgaccaaagg caccattacc attaataaca ttaactataa caaactggat cataaattag
    15661 cggcacaact cggtatcggg attatttatc aggaactcag cgttattgat gaattaaccg
    15721 tactggaaaa tttatatatt ggtcgtcatc tgacgaaaaa aatctgtggc gtcaatatta
    15781 tcgactggcg agaaatgcgt gtccgcgccg ccatgatgtt attacgcgtg ggcttgaaag
    15841 ttgatttaga tgagaaagtg gcgaatttat ctatcagcca caagcagatg ctggaaattg
    15901 ccaaaacgct gatgctcgac gccaaagtca tcatcatgga tgaacccacc tcctcactca
    15961 ccaataaaga ggtggactat ctgtttctga tcatgaatca gttgcgtaag gagggtacgg
    16021 ccatcgtcta tatctcgcat aaattggcgg aaattcgccg tatttgcgac cgctatacgg
    16081 tgatgaaaga cggcagcagc gtttgcagcg gcatggtgag cgatgtgtca aatgacgata
    16141 tcgtccgtct gatggtaggc cgcgaattgc aaaaccgttt taacgcgatg aaggagaatg
    16201 tcagcaacct tgcgcacgat acggtttttg aggtgcgcaa cgtcaccagc cgtgacagaa
    16261 aaaaggtccg ggatatctca tttagcgtct gccggggaga aatattaggc tttgccggac
    16321 tggtcggttc cggacgtact gaactgatga actgcctgtt tggcgttgat aaacgcgctg
    16381 gcggagaaat ccgtcttaat ggcaaagata tctctccacg ttctcccctg gatgccgtga
    16441 aaaaagggat ggcttacatc actgaaagcc gccgggataa cggctttttc cccaactttt
    16501 ctatcgctca gaacatagct atcagccgca gtctgaaaga cggcggctat aaaggcgcga
    16561 tggggttgtt tcatgaagtt gacgagcaac gtaccgctga agcccagcgc gaactgctgg
    16621 cgctgaaatg tcattcggta aaccagaaca tcactgaact ctccggcggt aaccagcaga
    16681 aagtcctgat ctccaaatgg ctgtgctgtc acccggaagt gatcattttc gatgaaccta
    16741 cccgtggcat cgacgttggc gcgaaagccg aaatttacaa agtgatgcgc caactggcgg
    16801 acgacggaaa agtcatcctg atggtgtcat ctgaactacc tgaaattatc accgtctgcg
    16861 accgcatcgc cgtgttctgc gaaggacgac tgacgcaaat cctgaccaat cgcgatgaca
    16921 tgagcgaaga ggagattatg gcatgggctt taccacacga gtaaaaagcg aagcgagcga
    16981 gaagaaaccg ttcaactttg cgctgttctg ggataaatac ggcacctttt ttatcctggc
    17041 gatcatcgtc gccatttttg gttcgctgtc gtcagaatat tttctgacca ccaataatat
    17101 tacccagatt tttgtgcaaa gctccgtgac ggtactgatc ggcatggggg aattcttcgc
    17161 tatcctggtc gccggtatcg acctttcggt tggcgcgatt ctggcgcttt ccggtatggt
    17221 gaccgctaaa ctgatgttgg caggtattga tccgtttctc gcggcgctaa ttggcggtgt
    17281 actggttggc ggcgcactgg gggcgatcaa cggctgcctg gtcaactgga cgggactaca
    17341 cccgttcatt atcacccttg gcaccaacgc tattttccgt gggatcacgc tggtgatctc
    17401 cgatgccaac tcggtatacg gcttctcatt tgacttcgtg aacttctttg ccgccagcgt
    17461 aattgggata cctgtccccg ttatcttctc gctaattgtc gcgctcatcc tttggtttct
    17521 gacaacgcgt atgcggctcg gacgcaacat ctacgcactg ggcggcaaca aaaactcggc
    17581 gttctattcc gggattgacg tgaaattcca catcctggtg gtgtttatca tctccggtgt
    17641 ttgtgcaggt ctggcaggcg tcgtctcaac tgcacgactc ggtgccgcag aaccgcttgc
    17701 cggtatgggt tttgaaacct atgccattgc cagcgccatc attggcggca ccagtttctt
    17761 cggcggcaag gggcgcattt tctctgtggt gattggcggg ttgatcatcg gcaccatcaa
    17821 caacggtctg aatattttgc aggtacaaac ctattaccaa ctggtggtga tgggcggatt
    17881 aattatcgcg gctgtcgccc ttgaccgtct tatcagtaag taaggaattg aacatgaaaa
    17941 tctccccctc gttaatgtgt atggatctgc tgaaatttaa agaacagatc gaatttatcg
    18001 acagccatgc cgattacttt catatcgata tcatggacgg ccactttgtg ccgaatctga
    18061 cgctctcacc gttcttcgta agccaggtaa aaaaactggc gagtaaaccg ctcgactgcc
    18121 atctgatggt aacgcgtccg caggattaca ttgctcaact ggcacgtgcg ggagctgatt
    18181 tcatcacttt gcatccggaa actatcaacg gtcaggcatt ccgcctgatt gatgaaatcc
    18241 gccgtcatgg catgaaagtg gggctgattc ttaatccgga aacgccggtt gaggccatga
    18301 aatactatat ccataaggct gataaaatta cggtcatgac tgtcgatccc ggttttgccg
    18361 gacaaccctt cattcctgaa atgctggata aagttgccga actgaaggca tggcgtgaac
    18421 gagaaggtct ggagtacgaa attgaggtgg acggttcctg caaccaggca acttacgaaa
    18481 aactaatggc ggcaggggcg gatgtcttta tcgtcggcac ttccggcctg tttaatcatg
    18541 cggaaaatat cgacgaagca tggagaatta tgaccgcgca gattctggct gcaaaaagcg
    18601 aggtacagcc tcatgcaaaa acagcataac gtcgtagcgg gcgtggatat gggggcaacg
    18661 catatccgct tttgtctgcg gacagcagaa ggtgaaacgc tacactgcga aaaaaagcgg
    18721 accgcagaag ttattgctcc cgacctggtg tcgggtatcg gcgaaatgat tgacgagcaa
    18781 ctcaggcgct ttaacgctcg ctgtcatggt ctggtgatgg gatttccggc gctggtcagt
    18841 aaagataaac gcaccattat ttctacgcct aacctgccgt taacagcggc agatttatat
    18901 gatctcgccg ataagctcga aaatacgctg aattgtccgg ttgagttttc ccgcgacgtt
    18961 aacctgcaac tctcctggga cgtagtagaa aaccgcctta cgcaacaact ggttctggca
    19021 gcctatctcg gtacggggat ggggttcgca gtgtggatga acggtgcgcc gtggacgggt
    19081 gcacacggtg tggcaggcga actgggccat atccccctgg gagatatgac ccaacactgc
    19141 gcgtgtggca attctgggtg cctggaaacc aattgctctg gaatggcgct aagacgctgg
    19201 tacgaacaac agccccgaaa ttacccattg agcgatcttt tcgtccatgc ggaaaacgcc
    19261 cctttcgtcc agagtctgct tgaaaacgcg gcacgagcca ttgccaccag cattaatctg
    19321 ttcgatcccg atgcggtgat tctgggcggt ggcgtgatgg atatgcccgc cttcccacgc
    19381 gagactctca ttgccatgac ccaaaagtac ctgcgccgtc cactgccgta tcaggttgtg
    19441 cgctttattg ccgcctcatc ttctgacttt aatggcgctc agggtgcagc aatattggcg
    19501 catcaacgtt ttttgccaca gtcctgtgct aaagtcccat gagtaaagcg ttggcacagt
    19561 agcgcaatgt cccgctgtgc caacgacagg tacaatatat ataataatta cataaaacag
    19621 cccatattat tcccagcaat gatagttcta ctgtaatttt ccctcttcag cacaaatgcc
    19681 taataaaatg gctcgttccg ctcacacttc cacttattaa agtcgcaatt tcagaattat
    19741 gataacgcca gctttaaatg acgtattttg catggattac atatttacga cataaatgat
    19801 aaatggagat ttatatgaat aactctcggt tattccgttt gagcaggatt gttattgcgt
    19861 tcactgccgc cagcggcatg atgataaata ccgcttacgc aacagatgaa gcgaaagccg
    19921 ccactcaata tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc
    19981 gtcaggattt tgacgatgcc cagcgtggat ttatcgctcc gctgctggat gaaggtattc
    20041 tgcgtgatgc gaacggtaaa atttactacc gcgcgaatga ttacaaattt gatattaatg
    20101 ccgctgcgcc ggaaaccgta aaccccagcc tgtggcgtca gtcgcagatc aacggcattt
    20161 caggcctgtt caaagtcacc gataaaatgt atcaggtgcg cggccaggat atctctaaca
    20221 ttacgttcgt tgagggcgag aaaggcatta ttgttattga cccactggtg acgccacctg
    20281 ccgcaaaagc cgcacttgac ctttacttcc agcatcgccc gcaaaaaccc attattgccg
    20341 ttatctatac ccacagccac gccgaccact atggcggcgt gaaaggcatc atctctgaag
    20401 ccgatgttaa atccggaaaa gttcaggtga ttgctccagc tggctttatg gacgaagcca
    20461 tcagcgaaaa cgtgctggcg ggtaacatca tgagccgccg tgcgctctac tcttacggtc
    20521 tgttactgcc gcacaacgcg caaggcaatg tgggtaatgg ccttggcgtg acgctggcaa
    20581 cgggcgaccc gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga
    20641 ttatcgacgg cctggagttt gacttcctga tgaccccagg tagcgaagcg ccagccgaaa
    20701 tgcacttcta tattccggcc ctgaaagccc tgtgtaccgc cgagaacgcc acgcataccc
    20761 tgcacaactt ctacactctg cgcggcgcga aaacccgcga caccagcaag tggaccgagt
    20821 atctgaacga aacgttggat atgtggggta acgacgcgga agtactgttt atgccgcaca
    20881 cctggccggt ctggggcaat aaacatatca atgattatat tggtaaatat cgcgatacta
    20941 tcaagtacat tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa
    21001 tcggcgacat gattaagctg ccgcctgcgc ttgccaataa ctgggccagc cgcggctatt
    21061 acggttctgt cagccacaac gcccgcgcgg tatataactt ctatcttggc tattacgacg
    21121 gtaacccggc taacctgcat ccgtatggtc aggtggagat gggtaaacgt tacgtgcagg
    21181 cgctgggcgg ttctgcccgc gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt
    21241 atcgctggtc ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg
    21301 cgaagaatct gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat
    21361 ggcgcggttt ctacctgacc ggcgcgaaag agctgcgcga aggggtgcat aagttcagcc
    21421 acggcaccac cggttccccg gacaccattc gcgggatgtc ggtcgaaatg ctgttcgact
    21481 ttatggccgt tcgcctcgat agcacgaaag ccgcaggtaa aaatatcagc ctgaacttca
    21541 atatgagcaa cggcgataac cttaacctga tgctgaacga tagcgtgctt aactaccgta
    21601 aaacgctgca atcgcaagcc gacgcctctt tctacattag ccgtgaagat ctgcacaccg
    21661 tgctgaccgg acaggctaaa atggccgatc tggtgaaagc gaagaaagcc aaaattattg
    21721 gcaatggcgc gaaactggaa gaaattatcg cctgtctgga taatttcgat ttgtgggtga
    21781 atatcgtaac cccaaattaa tgtcactcgc ttttgggggc gctgaagccc ccaaaagcat
    21841 ttattttaaa aatatataca gcaaattaat acataaaata tataagttat gaatgcggtg
    21901 gctgaataaa gctattttta ttcatcaaga aaatagccat actgacgcct tcaatggtta
    21961 ttgatattca ctacagatat tgttcgcagc agacaggatg tatttaatat gccaacagtt
    22021 ctctctcgca tggcgatgca actcaaaaag acagcctgga taattcccgt cttcatggta
    22081 tcgggatgct cattatctcc ggcgatcccg gtgatcggcg cttattatcc cggctggttt
    22141 ttctgcgcta ttgccagcct tattttgacg ctcatcacga ggcgaattat tcagcggaca
    22201 aatatcaatc tggcatttgt cggaattatt tataccgccc tttttgctct ctacgccatg
    22261 ctgttctggc tggcattttt ctaatctatt tgagacgcgc tcatggaaag tacgccgaaa
    22321 aaagctcctc gcagtaaatt ccctgctctg ttagtggttg cgttggcgct ggttgccctt
    22381 gttttcgtta tctggcgcgt agacagtgcg ccatcaacta atgacgctta cgcgtcagca
    22441 gataccattg atgtggtgcc ggaagtcagc ggtcgcattg tagaactggc agtcaccgac
    22501 aaccaggcgg tgaaacaggg cgatttgctg ttccgcattg atccgcgccc gtacgaagcc
    22561 aatctggcga aagctgaagc ctccctcgcg gcgctggata agcaaattat gctcacccag
    22621 cgtagcgttg acgcacaaca gtttggtgcc gactcggtta atgccacggt agaaaaagcc
    22681 cgtgccgccg cgaaacaagc cagcgatacg ttacgccgta ccgaaccgct actgcgagaa
    22741 ggttttgttt ctgcggaaga ggttgaccgt gcaagaaccg cacagcgtgc ggcagaagcg
    22801 gaccttaatg ccgtattgtt gcaggcgcag tcagccgcca gctccgtcag cggcgtggat
    22861 gcgctggtgg cgcaacgtgc ggcagtcgaa gcggatattg cgttgacgaa actgcatctg
    22921 gaaatggcga ccgttcgcgc gccgtttgat ggccgggtca tttccctcaa aacctccgtc
    22981 gggcagtttg catctgcgat gcgccctatt ttcactctaa tcgacactcg tcactggtac
    23041 gtgattgcca acttccgcga aaccgatctg aaaaatattc gctcgggtac actcgcaacc
    23101 attcgcctga tgagcgacag cggcaaaacc ttcgagggta aagtggattc gattggctac
    23161 ggcgtgctac cggatgacgg cggcctggtg ctgggcggcc tgccgaaagt gtcacggtct
    23221 attaactggg tccgcgttgc ccagcgtttt ccggtcaaaa tcatggttga taaacctgac
    23281 ccggaaatgt tccgcatcgg cgcttcggca gtcgctaatc ttgagccaca ataatgagcg
    23341 cgctcaactc cctgccatta ccggtggtca ggctgctggc gttctttcat gaagagttaa
    23401 gcgagcggcg accagggcgc gtgccacaga ctatgcaact ctgggtaggc tgcctgctgg
    23461 tgattctgat ctcgatgact tttgagatcc cttttgtggc gttatcactg gcagtgctgt
    23521 tttacggtat tcagtcgaac gcgttttaca ccaaatttgt cgcgatcttg tttgtggttg
    23581 ccacggtgct ggagatcggc agcctgtttt tgatctacaa atggtcatat ggcgaaccgt
    23641 tgatccgatt gatcatcgcc gggccgatcc tgatgggttg catgtttttg atgcgcaccc
    23701 atcggctggg actggtcttt ttcgccgtcg ccattgtcgc tatttacggg caaaccttcc
    23761 ccgccatgct cgactatccg gaagtggtcg tgcgcttaac gctgtggtgt atcgttgttg
    23821 gcctctatcc aaccttgctg atgacgttaa tcggcgtgct gtggtttccc agtcgtgcca
    23881 ttacgcagat gcatcaggcg cttaatgatc ggcttgatga tgccattagc cacctgacgg
    23941 acagcctcgc accgctaccc gaaacgcgga ttgaaagaga ggcgctggcg ctgcaaaaac
    24001 tcaatgtctt ttgcctcgcg gatgatgcca actggcgaac ccagagcgca tggtggcaaa
    24061 gctgcgtggc aacggtaacc tacatttact cgacgctgaa tcgctacgat cccacctctt
    24121 ttgctgattc tcaggcaatt attgaatttc ggcaaaaatt agtctcagaa atcaacaagc
    24181 tgcaacatac cattgccgaa ggtcagtgct ggcaaagcga ctggcggctt agtgaagatg
    24241 aagcggtggc ggcacgagaa tgtaacctgg agaatatctg ccagacgtta ttgcaactgg
    24301 gtcagatgga cccgaatacg ccgcctacgc ctgcagccaa gccgccatca atggtcgccg
    24361 atgcctttac caatccagac tatatgcgct acgcggtaaa aacgctgctc gcctgtttga
    24421 tctgttacac cttctacagc ggcgtggact gggaaggcat tcacacctgt atgctgacct
    24481 gcgtgatcgt cgctaatccc aatgtcggtt cgtcgtacca gaagatggtg ctgcgttttg
    24541 gcggggcctt ttgcggcgcg attctggcgc tgttattcac gttattggtc atgccctggc
    24601 tggacaatat tgtcgaattg ctgtttgtgc tggcaccgat tttcctgttg ggcgcatgga
    24661 ttgccaccag ctctgaacgc tcttcttata tcggcacaca gatggtggtc accttcgcgc
    24721 tcgccacgct cgaaaacgtt tttggcccgg tgtacgacct ggtggaaatt cgcgatcgcg
    24781 ccctgggtat catcattggt accgtggtgt ccgcggtgat ttacaccttt gtctggcctg
    24841 aaagcgaagc gcgcacgctg ccgcaaaaac tggctggcgc gctgggtatg ttgagtaaag
    24901 taatgcggtt cccacgccag caggaagtca cggctctgcg cacttatctg caaattcgca
    24961 taggtctgca tgcggcgttt aatgcctgtg aagagatgtg ccaacgcgtg gtgctggagc
    25021 gtcaactgga cagcgaagaa cgcgcattac tgattgaacg ttcgcaaacg gttattcgtc
    25081 agggccgcga tattcttcac gcctgggatg ccacctggaa ctcggcgcag gcgctggata
    25141 acgcactaca gccggacaga gcaggtcagt ttgccgacgc cctggagaaa tacgccgccg
    25201 gtctggcaac cgcactcagc cgttctcctc aaataacgct tgaagaaaca cccgcctcgc
    25261 aggccatcct gccgacctta ttaaaacagg agcaacacgt ctgccagctt ttcgcccgct
    25321 tgccagactg gacagccccg gcattaacgc ccgccacgga acaggcacaa ggagccacgc
    25381 aatgatcaat cgtcaacttt cacgtctgct gttgtgcagc attctcggca gcacgacgct
    25441 gatttccggc tgtgccctgg tacgtaagga ttctgcgcct catcaacagc tcaaaccgga
    25501 acaaatcaaa ctggccgatg atattcatct tgccagctcc ggctggccgc aggcgcagtg
    25561 gtggaaacaa ctcaatgacc cgcagctgga tgcgctgatc caacggacgc taagtggttc
    25621 acacaccctc gccgaagcga aactgcggga agaaaaagcg cagtcccagg ccgatttgtt
    25681 agatgccggt tcacaattac aggtcgcagc gttagggatg ctcaaccgcc aacgcgtctc
    25741 ggcgaacggc tttttaagcc cttatgcgat ggatgcgtca gcactgggta tggacgggcc
    25801 gtactatacg gaagccacag tcggtttgtt tgccggactg gatctcgatt tgtggggtgt
    25861 gcatcgctca gcggttgctg ccgccattgg cgcgcataat gccgcgctgg cagaaaccgc
    25921 agcagtagag ctatcgctga ccacgggcgt agcgcagctt tattacagta tgcaggccag
    25981 ctatcagatg ctcgatctgt tagaacaaac tcgcgatgtg attgattacg cggtgaaagc
    26041 gcaccagagt aaagtggcgc acggtctgga agcgcaagtg cctttccacg gcgcgcgggc
    26101 acagattctg gcggtcgata aacaaattgc tgccgtcaaa gggcaaatca ctgaaacgcg
    26161 agaatctctg cgtgcattga ttggcgcggg agccagcgat atgccggaga tcaaaccggt
    26221 ggcattacca cgagtacaga ccggcattcc ggcaacgctc tcttatgagt tgctcgccag
    26281 acgcccggat ctgcaagcca tgcgctggta tgttcaggcg tcattagatc aggtggattc
    26341 cgcgcgggcg ctgttctacc cgagctttga tatcaaagcg tttttcggtc tggactccat
    26401 ccacctggat accttattca aaaaaaccag tcgccagttc aacttcatcc cgggtctgaa
    26461 attgccgctg tttgacggtg gacggttgaa tgccaatctc gaaggcactc gcgccgccag
    26521 caacatgatg attgaacgtt acaaccagtc agtactgaac gcggtgcgtg acgttgccgt
    26581 caacggcaca cgtctgcaaa cgctcaacga cgagcgagag atgcaggctg aacgcgtaga
    26641 agcaacgcgc ttcacccagc gcgcagccga ggccgcctat cagcgcggct taaccagccg
    26701 cttacaggcc accgaagccc ggttgccagt gcttgccgag gagatgtcat tactgatgct
    26761 ggacagccgc cgggtgatcc aaagcattca gttgatgaaa tcgctgggcg gcgggtatca
    26821 gacggctccc gtcgtcgaga aaaaataaaa tgtctaccgc gtgatggctg tcacgcggta
    26881 tttcgtttcg tcacgtcaaa actgacgaca gcctgttttt cgtcagagtt ttgaataaat
    26941 agtgcccgta atatcaggga atgaccccaa ataaaatgtg gcataaaaga tgcatactgt
    27001 agtcgagagc gcgtatgcgt gatttgatta actggagcga gaccgatgaa aaaagtcgtc
    27061 acggtttgcc cctattgcgc atcaggttgc aaaatcaacc tggtcgtcga taacggcaaa
    27121 atcgtccggg cggaggcagc gcaggggaaa accaaccagg gtaccctgtg tctgaagggt
    27181 tattatggct gggacttcat taacgatacc cagatcctga ccccgcgcct gaaaaccccc
    27241 atgatccgtc gccagcgtgg cggcaaactc gaacctgttt cctgggatga ggcactgaat
    27301 tacgttgccg agcgcctgag cgccatcaaa gagaagtacg gtccggatgc catccagacg
    27361 accggctcct cgcgcggtac gggtaacgaa accaactatg taatgcaaaa atttgcgcgc
    27421 gccgttattg gtaccaataa cgttgactgc tgcgctcgtg tctgacacgg cccatcggtt
    27481 gcaggtctgc accaatcggt cggtaatggc gcaatgagca atgctattaa cgaaattgat
    27541 aataccgatt tagtgttcgt tttcgggtat aacccggcgg attcccaccc aatcgtggcg
    27601 aatcacgtaa ttaacgctaa acgtaacggg gcgaaaatta tcgtctgcga tccgcgcaaa
    27661 attgaaaccg cgcgcattgc tgacatgcac attgcattga aaaacggctc gaacatcgcg
    27721 ctgttgaatg cgatgggcca tgtcattatt gaagaaaatc tgtacgacaa agcgttcgtc
    27781 gcttcccgta cagaaggctt tgaagagtat cgtaaaatcg ttgaaggcta cacgccggag
    27841 tcggttgaag atatcaccgg cgtcagcgcc agtgagattc gtcaggcggc gcggatgtat
    27901 gcccaggcag aaagcgccgc catcctgtgg ggcatggggg taacccagtt ctatcagggc
    27961 gtggaaaccg tgcgttctct gaccagcctc gcgatgctga ccggtaacct cggtaagcca
    28021 catgcgggtg taaacccggt tcgtggtcag aacaacgtgc agggtgcctg cgatatgggc
    28081 gcgctgccgg atacgtatcc gggctatcag tacgtgaaag atccggctaa ccgcgagaaa
    28141 ttcgccaaag cctggggcgt ggaaagcctg cctgctcata ccggctatcg catcagcgag
    28201 ctgccgcacc gcgtggcgca tggtgaagtg agagcggcgt acattatggg cgaagatccg
    28261 ctgcaaactg acgcggaact gtcggcagta cgtaaagcct ttgaagatct ggaactggtt
    28321 atcgttcagg acatctttat gaccaaaacc gcgtcggcgg cggatgtcat tttaccgtca
    28381 acgtcgtggg gcgagcatga aggcgtgttt actgcggctg accgtggctt ccagcgtttc
    28441 ttcaaggcgg ttgagccgaa gtgggatctg aaaacggact ggcaaatcat cagtgaaatt
    28501 gccactcgta tgggttatcc gatgcactac aacaacaccc aggagatctg ggacgagttg
    28561 cgtcatctgt gcccggattt ctacggtgcg acttacgaga aaatgggcga actgggcttc
    28621 attcagtggc cttgccgcga tacttcagat gccgatcaag gaacttctta tctgtttaaa
    28681 gagaagtttg ataccccgaa cggtctggcg cagttcttca cctgcgactg ggtagcgcca
    28741 atcgacaaac tcaccgacga gtacccgatg gtactgtcaa cggtgcgtga agttggtcac
    28801 tactcttgcc gttcgatgac cggtaactgt gcggcactgg cggcgctggc tgatgaacct
    28861 ggctacgcac aaatcaatac cgaagacgcc aaacgtctgg gtattgaaga tgaggcattg
    28921 gtttgggtgc actcgcgtaa aggcaaaatt atcacccgtg cgcaggtcag cgatcgtccg
    28981 aacaaagggg cgatttacat gacctaccag tggtggattg gtgcctgtaa cgagctggtg
    29041 accgaaaact taagcccgat tacgaaaacg ccggaataca aatactgcgc cgtgcgcgtc
    29101 gagccgatcg ccgatcagcg cgccgccgag cagtacgtga ttgacgagta caacaagttg
    29161 aaaactcgcc tgcgcgaagc ggcactggcg taataccgcg taaactcacg ttaaaagcgg
    29221 cgatattacc caacgccttt gtatcaatcc ggaaacgact ctctgtttcc ggattttttt
    29281 atacatacct gcgcagtcaa accagcccat cctggttatt agcgacgtct tctcggttgt
    29341 tcttctattc cagtcacgat tgcaaatgag aattatcttt actcaattct catcgcggcc
    29401 taacgtaata atactttaca tttgataaac aaaatttcgg taaggtgaga aatcatcgac
    29461 cgtttaactc gttctattaa cgggatattc tggcgcgatt aatgcagcga aaccccgtct
    29521 gatagcatat taataacctc aaagtaattt cagggattgg gaagcatatg attaaaaaac
    29581 tattaccttt actggtttta agcacaattt ccgccacaac cgttgccgct acaccgccta
    29641 atacgttggt tgtagcccag gggctggatg atattgtgag tctggacccg gcagaagcta
    29701 atgaactctc aagtattcag actgtaccaa gtctgtatca acgcgtagta caaccggacc
    29761 gtaatgatcc gacgaaaatc actccgattc tggcggaaag ttggcaggct gaccccgcag
    29821 ccaaaacgct aaccataaaa ctgaaaagcg atgcgaaatt tgcctctggt aatccggtac
    29881 gcccggaaga tgtgatttac tcttatgtcc gggctgtcac actgaataaa tccggtgcgt
    29941 ttattctcaa tgtgctggga tggcagccgg aaaatatcgc cagccagtta aagaaaatcg
    30001 atgaccatac cgtccaggta caatggagtg ccgacgtcag cccggcgctg gcactgaata
    30061 ttctctccac accaatcgcc tcaattgttg acgagaagct ggttgcgcca aacgccaaaa
    30121 ataacgactt tggtaatgaa tggctgaaga tgcattccgc tggcagcggc gcgtttaaaa
    30181 tgcgtactta ccaaccgcat caggccatcg tgatggaagc caacgccagc tcaccaaccg
    30241 gcgccccgaa actgaaaagc gtcatcatca aaaatgttcc cgatccggca acacgtcgcc
    30301 tgctgatcca acaaggtgat gcggatatgg ctcgtgacct gggagccgat caaatcgatg
    30361 cattacaggg taaacctggc gtaaaagtga tgagcattgc ctccgccgaa cagaactacc
    30421 tggccttcaa taccggaaac aaggataacc cgctgatgag taatcctgcg ttctgggagg
    30481 ccgctcgctg gttggtcgat tacgacggca tcaccaaaaa tctcttaaaa ggacaatatt
    30541 ttacccatca aagtttcctg cccgtgggct ttcctggagc actggaagaa acaccgttta
    30601 cctttaatcc agccaaagca aaagagatcc tcgctaaagc cggaatcaaa gacccgcact
    30661 tcacgcttga tgtcgaaaat aaaccgccat ttattaccat cgcgcagtca attcaggcca
    30721 gctttgctca gggcggtgta aaagttgatt tgctgccagc cgcagggagt caggtctact
    30781 ctcgcgttcg cgcccaccag catcaggggg ccattcgtat gtggttgcct gattattttg
    30841 acgcacattc caacgccagc tctttcgcct ataacgatgg caaatccagc accgttgccg
    30901 ggttaaacgg ctggaaaatc ccggaactga acaaagaaac gctggcggcc atcgctgaac
    30961 ctgatagcac taaacgtctt gatttgtata agaaaatgca acaagaacta caacgtagtt
    31021 ccccgtatgt ctttattgat caaggcaaaa cgcaaattgt catgcgtgat aatgtgcagg
    31081 ggtatcaaca agggttgaat gccgatatgg tctggtttga tcaggtaacc aaataaccct
    31141 gtatgcaact cgcgtccagc catgttaccg ggcgcggtac cagggctggc ctgtattgca
    31201 ggccagccgc tgatttatca ctgtatggaa agatcatgtc tgtcgttttt tccactcgcg
    31261 cgagatggcg aggcaagcgc cctctgctcg cgctgttgca agggctgttt accgttgctc
    31321 tgacgctact cggcctgctg ctgattacct ttgcgctttc ggctctctca ccggtcgatc
    31381 gcgtactaca aatcgttggc gatcacgcca gccagtccac ctatgatcag gtacgccacc
    31441 agttaggatt ggatcagccg ttagccgtcc agttctggca ttacctgatg aatctggcac
    31501 acggcgatct gggtatcgcc agctccaccg ggcaaccagt actccaggac ttgctatcag
    31561 cattcccggc gaccattgaa ctggcaacgt tggcgcttat tgtcggtgct gttgtcggcg
    31621 tcattgcggg tgtactttgc gcccgttatg tcggttctcc gctggatttc accgtgcgaa
    31681 cattaactct actgggaaat tccgtccctg ttttttggct tggtctgctg atgctggcgc
    31741 tgttctacgc cagactgcaa tggagtgccg ggccgggcag gctggatgat atttatcaat
    31801 tcaccataga accccgtagc ggatttgcgc tgattgatac gtggctttct ggcgatcgcg
    31861 aggcatttac caatgcaatc aaccatttga tacttccggt tttattactg gcgtacttct
    31921 cgctcgccag tattacgcgc ctgactcgtt ccgcctgtct tggcgaaatg aataaagaat
    31981 atattctgct ggcgcgcgcc aaaggagcca gcgagatgac aattttgcta cgtcatgttt
    32041 tacccaacat tcgcggcacc ttgctcactg ttatcgccct cgcctataca tcaatgctgg
    32101 aaggcgcggt actgacagaa accgtctttt catggccagg tattgggcgc tacctcacta
    32161 cagcgctctt cgcgggtgat accacagcaa tcatgggagg aacgctgctg attggcgtct
    32221 gctttgtgtt gataaataac ttaaccgacc tgctggtccg gctgaccgat ccgaggttac
    32281 gctgatgccg atgtttctct ttttacgccg tttacgccat tcacccgccg cgttttgcgg
    32341 gttgataatt atcctgttat tgatgctaat tgcgctcttc gcgccgtggc tggcacctca
    32401 cgatcctaac tggcaggatg cggcggcgcg gttacaagca ccgagtcgtc agcactggct
    32461 cggcactgac agttacgggc gagacctact ttcacgttta atctacggca gccgtccggc
    32521 actggggctg gtggcattgg tgacaatcat tacactcccg gtcggtctgc tgattggcat
    32581 tttagccggg tactacggcg gctggctgga acgcgtgctg atgcgtttta ctgacgtagt
    32641 aatgtcaatg ccgcgtttaa ttctggcttt tgcttttgtt gccatgctgg ggccggggct
    32701 ggttaacggc gcactggcgc tggcattaac cacctggcct gcttatgcgc gtcaggcacg
    32761 gagtgagatc caacgtctgc gacacagtga ttatcttgcc gccgcagaaa tgatgggcat
    32821 ccgcggcagg cgtctgctgg ctggtcatat tttgccattg tgcctgccat ctgccattgt
    32881 ccgtctggcg ctggatttag cgggtatcat tctggcggct gctggcctgg gttttcttgg
    32941 gcttggtgct cgcccgccaa tggcggaatg gggagccatg accgccgacg gtatgcaggt
    33001 catttttgat caatggtgga ttgccgccgc tcccggcgca attatcttaa ttgccagtct
    33061 ggcctttaat ctgctgggtg atggcttgcg cgatgttctg gagcctcaac atgactgact
    33121 cacgtcttag tatcaacaat ctctgcgtcg attatccgga gtcccgggtc gtcaacaatg
    33181 tcagttttac attaggcaat gaacgtctgg cgctggtcgg tgagtcgggt tcaggtaagt
    33241 ccatgaccgc ccgcgccctg atggggctgg tgcgtaaacc gggtgtagta accgcggaca
    33301 cacttaacat tctggggcgt gatgcactca cccttaacgc gcgtggatgg caacagttgc
    33361 gcggtaacga tatcgcgatg gtattgcaag atccgcgtta cgcgcttaat ccagtaaaga
    33421 cagtaaaagc gcaactggaa gaggcgctta cgctccatca acgtctgaac cgccgcgaga
    33481 aagaagagaa aataaacacc gcaattcagg ctgtcggcct caatacgcga gtactgcaaa
    33541 gctatcctcg tgaactttct ggcggcatgg ggcagcgagt gatgattgca attgcgctca
    33601 tcaacgatcc ccgggtattg attgccgatg agccaacatc agcactggat gtgcgcctgc
    33661 gtaatcagat tctggaactg ctggttatcc agtgtgaaca acgtcagatg gcaatgttgc
    33721 tgatcagtca cgacctgcca ttagttgccc aattttgcca tcgggtgctg gtgatgtatc
    33781 agggaaataa ggtggatgaa atgcacgctg ctgcgctacc tacagcaacg catccttaca
    33841 cgcgcacact gtggacttgc cgcccaaatg cacaaactta cgggcagatg ctgccgacgc
    33901 tggacaggac ggcaatgacg ccggagaaat accatgacga ttgttgaaat taatcactta
    33961 caggtgactt ttgctgagaa aaccgcagtt tctgccgcca gtttttcggt taatgccgga
    34021 gagacattta gtttaattgg cgaatcaggc tgcggaaaat ccacgatttt gcgcgtaatt
    34081 gctggattac agcgcgactg gcacggtcag gtgtcactgt ttgggcacac catcaaacca
    34141 ggaatgcgtt ttcagggcga tctccgccgc aatgtgcaaa tggtgtttca ggatccctat
    34201 gcctcgttgc atcccaatca tactctgtgg cgaacgctgg ctgaaccact caaaattcgt
    34261 ggcgaaagag aaatagaaaa acgcgtccag actgcactgg aacaggttgg cttgcctttc
    34321 gacgccgctc gtcgctaccc acaccagctt tccggtggtc aacggcaacg tgtggttatt
    34381 gctcgcgccc tgttgttacg accacaactt cttctgctgg atgaacccac ctcggcgttg
    34441 gatatgtcgg tacaagcgga aatccttaat ttgttgaatc aacttaagct ggaacaccaa
    34501 atgacatatc tgctggtcag tcacgatgcc gatgtcatcg cgcatatgtc tgatcgagcg
    34561 gcattgatgt ccgagggaaa aattcagcga ttttttgatc gcgacgccat ggaaaagggg
    34621 gaacatcgga tggattaaaa aacctgacat tgcattaatt cattctgctt tcaggtcttt
    34681 ttacagttgt ttttttgtcc acccgaacac tttatactgt ctctccccta ccaaacttca
    34741 ttatcaaaat gaaaaaaatt atcactttga tgttgttttt gacattcttt gcccacgcga
    34801 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggagac cgacgcgcac
    34861 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
    34921 cacagaaagc cgccgacagc ggtgatgccg atgcctgcgc gctgttggcg cagatcaaaa
    34981 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt gcttgccgag aaagcagcgc
    35041 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat acgcaagcgg
    35101 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
    35161 acgactctgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
    35221 ttaaggccga cgatgacaaa gcaacctggt atttcaaacg tagctccgcg atttcccgaa
    35281 ccggttattc cgaatactgg gcgggaatga tgttcttaaa cggtgaagag ggttttatcg
    35341 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
    35401 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcgcagc aaacgtcgca
    35461 tcaggcgttg atgtcggatg cggcgtaaac gccttatccg acctacaaaa tcacgctaca
    35521 tcaggctatt gcgctgtctg acgtaggcct gataagacgc gttaagcgtc gcatcaggcg
    35581 ttgatgtcgg atgtggcgta aacgccttat ccgacctaca aaaaccacgc tacatcaggc
    35641 tattgcgctg tctgacgtag gcctgataag acacgtcagc atcgcatcag acattgatgt
    35701 cggatgcggc gtaaacgcct tatccgacct acaaaatcac gctacatcag gctattgcgc
    35761 tgtctgacgt aggcctgata agacgcggca agcgtcgcat caggcgttga tgtcggatgc
    35821 ggcgtaaacg ccttatccga cctacaaaaa ccacgctaca tcaggctatt gcgctgtctg
    35881 gcgtaggcct gataagacgc ggcaagcgtc gcatcaggcg ttgatgtcgg atgcggcgta
    35941 aacgccttat ccgacctaca aaaatcacgc tacatcaggc tattactcag tcaggcatcc
    36001 acacattgcc gggtggatat ccccccggca atcttcaatt actgatccgc agttttatca
    36061 aatttgccca gtacttcacg ctcataagcc agcgctttct tacggtcaaa tttgtgttcc
    36121 catttggcaa tcaccagcac cgccagcgca ttacccacca cgttcagcgc agtacgcgcc
    36181 atgtcgagga tacggtcaac accggcaata aacgccagac cttccagcgg gatccctacg
    36241 ctgcccagcg ttgccagcaa caccacaaac gacacgcccg gcacgccagc aatccctttc
    36301 gaggtcacca tcagcgtcag caccagaatg atttcctgcc agatggagag gtcaatgcca
    36361 tacagctgag cgatgaaaat agcggcaata ctttgataca gcgtcgaacc atcaaggtta
    36421 aaagagtaac cggtcggcac cacgaaactg gtgatcgacg ccggtgctcc gtaggcttcc
    36481 atcttctcaa taattcgcgg cagcacgctt tcagagctgg cagtggagta cgccagaatc
    36541 agctcatctt tcagaatacg aatcaggatc cagacgctta acccgcacag gcgcgccaca
    36601 attcccagta cgaccagcgc gaagaacaga atggcaaaat gcaccagcag caccagtttc
    36661 gccagtggcc acagagaaga gaaaccaaag ttagccaccg tcaccgcaat cagcgcaaac
    36721 acacccaccg gcgcataacg catcaccatg tgagtcactt taaacatggt ttcagagatg
    36781 gagcggaaaa cggtcaccag cggttcacga tgcgtcgcgg gcagggatga aagccccaga
    36841 ccaaacagca ccgagaagaa gatgatcggc agcatttcgc ctttcgccat cgacgccacg
    36901 atgttcgtcg gcaccagcga caaaatcgtg cccataatgc cgtgggaact gctttgtacc
    36961 gcttccgtag tgctctgata tttcgagata tcgacggtcg ccagctgcga catatccacc
    37021 ccggcaccgg gctggaagac gttcgccaga gtgatcccca aaatgatggc gacggtggtg
    37081 atcacctcga agtagataat ggttttcgca ccaatacgcc cgagctgttt ggcatcacca
    37141 acacccgcga tacccactac cagtgtggag atcacaatcg gcacaacaat cattttgatc
    37201 agatggatga agatatcacc cgccggagag agcaaattga caaccagcca gtcgcggctg
    37261 tcgctatggt agtgcaggta gcttcccagg agaatgccca gcaccatagc aaacagaatc
    37321 tgccaggcca ggctgaattt tatatttttc atcagaataa cttcctcaat ggagcgccgt
    37381 tccccgttca gaaacgcgag aacgtgacat actgccaggg catgaattgt gttgcgttgg
    37441 tttgtagatt ttttaacgct tatgatcagt atcatttgcg cggtatgttg cgcaaccctt
    37501 taacaacaga gcttttcgct gcattaatgc ataataacgc gagtttatgg caggattaat
    37561 gaataaccat ttgttataga aggattttta tttgtttaat ctgcaataat actgattggt
    37621 gattattttt gattatatgt tcattcgatt attttttatg cagctcaata acataatctt
    37681 catcaccaga tgattaatct gatctccgct gcaacaattt cgccatatta atcgactcga
    37741 ccagctgtgt ccggttgatc cggggtgagt tgagatccat cactttttgc catagttcga
    37801 tggcttgcgc gtaattcgcc tgcataaacg catcggaagc cagcagcatc agagcggtga
    37861 tttcgttaga gtccagcgcg agggctttgt cgatcattgc gcgagtctgg acggtcatat
    37921 gttggctggc ctggtaataa agcaccgtcg ccagcgccgc atacagttca gcgttctcac
    37981 cacgtagttg caacgcctga cggtacgcca gcagcgaatt gctgtaatcg ttttgccaca
    38041 gataatactc gcccagtaac gcccactgtt tgctgttttg tggattagca cggattttat
    38101 cctgcaatgc ctgaagctgc gcttccgggg tttgctggct ggcaaactga tgcagcggat
    38161 cgcgctgacg ctgatactcc gcacgtaccg cctgccattt tggacttaac agataactgc
    38221 cgacacagag aaataccatc aaaatcgtga gtgttgtcag cattttcacc ggtatttggg
    38281 gctgtttcat cgcttcgccc tcactcgcca gaggatcagt gccatcagca gtaacaacac
    38341 tactggcagc gcccacaaca ccagcgtctg acccgttaac ggtgggttat agcgaacaaa
    38401 atctccgtag cgttcggtca tccagccaat gatttcgact tcacttttcc cctcttccac
    38461 catggtgtaa acctgatggc gcatactgac tgccaccggc gcattggatt ccagtaagtt
    38521 ctgattctgg cactgcggac aacgtaactg gctggcaata tttaacgcct gttgctgttg
    38581 ttgcggattg gcgaattgcc aggtgtctac aacctgagcg cgcgcaaaac aggtaaataa
    38641 cagcagtagc gtgagaagcc ctttattcat cgcgcttcct tccccgccat ccgcttaaca
    38701 atgcgcccga aatcatcaac aatcctcccc cccagatcca gcgcacaccg ctttgtacat
    38761 acaaacgaaa agcgtaacga tccgctccgg ttttctcgcc catgaccgca taccagtcat
    38821 ggatgccatt ccagcgaatt gacggttcca tcatttgctg acgacgtgct tcgtaaaaac
    38881 gccgctccgg cgttaattca ccaatgcgtt gctgatggtc aaacagtgcc actatcgctt
    38941 tttcgctggt gtaattgcct ttggcttgca gatcgaggcg ctcaaaacgg aaggtgtatc
    39001 ctgccagcgt cacctgctga cccggctgta aattcaggct gatctcctga cggctgacgc
    39061 tggaaaccac aatccccgcg gcaaataaca gcacgcccgc atgcgccagc agcgccggaa
    39121 gctgagcacg tttgccagag acaaacgtcg ccagcacaat caccaccagc atcaacagag
    39181 caaacggtaa cgtcgcgcgg ttaaaatacg gcgcgccaac ggagaggcgt ccccagccca
    39241 gcaggccgta aatcatcgga taaagcgttc ccaccagcac gatcagtagc actgcgcaaa
    39301 acagcaacag cgtagcgagg attaacattt cccgcgataa ccccgaaaaa cgcaccgccg
    39361 ggccaccgtc ccgcgctcgc cagccataca gagccagaga cgcaaggcta atcagtgcaa
    39421 acaggctgaa caacggcacg gcgcggacgt tatccagcgc gaacgcatga accgaaacca
    39481 gaatgccaga acggacaatt aaggtgccca gcagcgacag catcagagtt actatcgcca
    39541 gcaacagcga ccagtggcgg aaaatccccc gctggcgtgt caaagacaaa ctgtgtagca
    39601 acgcagtaga tgaaagccag ggtaataacg acgcgttttc aaccggatcc caaaaccacc
    39661 aaccgcccca gcccagttcg caataagccc accaggaacc gagaatgatc cctgcggtta
    39721 atgcgctcca gccaggtagt gcccaacgcc agcaaattcg ggcgcaggta ccatcaaact
    39781 cgccgcgcag taaactcgcc agcgccacgc tcgccgccac catcaaaccg ccgtagccga
    39841 gataaagcag cggtggatga aagataagac cgggatgttg cagcattggg ttgagatcgc
    39901 gcccttctat tgcgggcgga aatatcctca caaaaggatc ggaccatccg acaacaaaca
    39961 gcagcagtgc ggcaagtatc agcgataaaa ccgttaacgt gagcggaaat agcggatcgg
    40021 tttgctgtcg ataatgccag gcaaacagca cgctccaggc ggaaagcagc agcacccaaa
    40081 gcagcagcga accttcatga ccgccccaca ccgccgccag tttgagttcc cacgacagca
    40141 ggctatagct atgttgggcg acataaatga ccgagaaatc gctgatgaga aaacaatacg
    40201 tcagtacgcc aaaggcgagc agcaggagcg cgaactgcgc cagaatgccg atgcaagtga
    40261 gtcgcatcat ggcaggccag cgcaaccgca ctcccgcgaa ggccgtcaac ggggtcaaca
    40321 cgttgacccc gagacttaac aacaacgcca gaaaaccgac ttcaggaagg aatacgtcca
    40381 gaaaccacct cttttctggc ttacgccacc gtcaactgcc cggcgtagag aatgaaaaag
    40441 cgcagcatca acacgccgac caggctggcc ccacatgccg ccagcacggc aggaatgccg
    40501 gaactgcgat tgacccacgg tttgagcaac attggcacaa tcagccccag cccggcgaca
    40561 ccaagccaga accaccacgt ccagaaaccg ccgcccaatg ccgccaccag cgcacgcact
    40621 ttaccgtcat cgcccagcgc cagaccgaca aaaaacgcca ccagcaggaa gatttcaccc
    40681 cataccacgg ggatttccat acggtgtaca aagtgcgctt ccgtggaatg cgggttactg
    40741 cgttggcgta tcgccatggc gatcagcgcc accgccgcac cagacgagat gccggagaag
    40801 aggaacagca ccggcaggat cgggttattg aggaacggat acgatttcag cgccgacagc
    40861 agaaagccgg tataagcccc caacaacacc gccagcacca gcatcaatgt ttccagtccg
    40921 cgatgaacgg gcgtcagcag gctcagaacc ttttgcacga tccccagctt cggcaaccag
    40981 cgttgttgca gggcaagcaa atcatgttca aagattttcg ccagccacag caccagcacc
    41041 accatgtaaa gctgaaacag catcaccccc atcgacatca ccgaggtaaa actgtagtgg
    41101 aacatcagct tccagaaggt ccacggtctt gtcaggtgga agacgaggat caacagaccg
    41161 aggatcaccg cgccaggccc gacaatcagc gtggtgcgca acaacgtact gtctgcaccg
    41221 cccgcctgcg ggtagacacg acgtaacagc acggccagcg tcaccagacc ggcagaaata
    41281 ccaatcaaaa acaggtagat ggcaatcggc cagtcccaca ccagcgattc aaaatgaaat
    41341 gcggaagtct gcgtcattgg ctcacctccc cgtatttaaa cggtacgcgg tacagcttcg
    41401 gtttggttcc cagcgccagc ttgtagcggt aagtgggctt ctggcgcagc agttgcgaaa
    41461 tctcactgct gggatcgtcc agattgccaa acgtcagcgc cttcgtcggg caagcctcaa
    41521 cgcacgcggg cagcttaccg gcctgcaaat gggtcttacg gcagaaatcg catttgtccg
    41581 ccgtcttcgt gaccggatgg ataaagcgca cgcggtacgg acaggcggcg atgcagtact
    41641 gacaaccgac gcagagatcc ggattaacat cgacaatccc actggcagcg tcgcgaaaag
    41701 acgcaccggt cgggcaaacg tcaacgcacg gcgcatgatc gcagtgctgg caagacttac
    41761 ggaagaagcg atatttcaca tcaggaaatt cgccctgcgg ctcgctacga ataatcgtca
    41821 ggcgcgagac gccttccggc accttgttca cttcccgaca agcatccata caggcggtac
    41881 agccgatgca taacgactcg tcatgcacca tgccgtaacg caccccattg atattcaacg
    41941 ttttcgccac cacgcgtcct gcggtgccgc tgacggctgc cagcacgccg acgccggtga
    42001 gaaactggcg acgagaccag gtcatggctg ctccttaagc aacggaacag acgccgggtt
    42061 aaagttcgga ttggtgcgct gatcgctgtg gcaatcgacg caaatcttaa tccgtccttt
    42121 gtcacttaac gtctgcatcg tatcttgctg tggatgcagg gaatggcagc tggcgcacgc
    42181 cactttggtt acgtggacat cgtgcggcca gaacgctttt tgcaactgtt ccggcagatg
    42241 gcaggacata cagacgctgt tctgctctcc aaccttgtac atcggctcgt taaagcgcat
    42301 cacatccttc accccttcgc ggtgttgcgg tgatggctga ccgtggcagt tggtgcaggt
    42361 gaccggcagt ttgttattcg ggttgataac agaagcatgt ttgccatgca taccttcggt
    42421 atctggctta tgacagtcca gacaggcggc atccgggtta cgctgctggg taacttcgta
    42481 gcgatcatca gatgcctgcg ccgcaggggt agcggtaatt ccgttcaggc tccacaacag
    42541 gcccgacgcc agcaccccgg cagttaataa cgaacgtaat acgctcatat tcactccatt
    42601 gtgttttccg ccccttgcga ggcggaacgg ggttattggc ttaacagacc gtttttacgt
    42661 gcctgctctt cccactgcgg gatcaccgtt ttgatgaagt cctgcttctc ggccttgatt
    42721 tgctccatgt tcaggccaat ggcctgctgg gctttctctt tggtcgagat atccgggatc
    42781 tggatttcat gggttatgcc tttggtcgcc agcaggcgcg ccagtttggt gcgtgcatcc
    42841 gccgctttat ccatcgccgt accgagcatc ctcagacctt cttccggtgc gtgcatatga
    42901 atgccgtggg aagcgatcgc caaatcccaa cgccactggg catgacggat atcgtcctga
    42961 atcggcttca tttcggcttc cgttgcgcct gcatccagcg ccgctttcgc ttcgaagtga
    43021 gcgtgaacca gttgatcttc aaccttgatt ttcaggtcgt taatcgactg cttacgttcc
    43081 gcgaccactt tttgcaaggc agctttgtcc tgggtatggc agttcgcaca agtctgggcg
    43141 aagttatcaa acggattacc aattttatgg tcggtgtaga gtttgccttc ggcgttctgc
    43201 acttttggca tatggcaatc gatacaggtc acgttgtttt tgccgtgaat gcccgctgtc
    43261 caggtttcat attccgggtg ctgcgctttc agcattggcg ttttcgacag ggagttagtc
    43321 cagtcagaga aggcaatttt gtcgtaatac tgctccatat tttcgacttt catgccgtca
    43381 tcccacggga atttaaccgc tttgtttttg ccgtcgaagt aatactccac atggcactga
    43441 ccgcaaacca tcgattgctg gtcgaaacgt ccggctttct cgaaaggttt accaatggct
    43501 tccatcgcgc gagccgcata cggacgggaa agggttaact ccggtttgcc tttggcgaac
    43561 tctggtgagg cggtgttatg gcaatcggca caacctaagt tgttgacgat ttccgggccg
    43621 ccgcgcgccc attttccgtg gaagtagcca tcttcgccgt ctttctggat cagacgcgcc
    43681 acatccgggc ttttacaact ccagcacgcc atcggtagcg gaccatcttc agcgtttttc
    43741 ggcgcaccgg tacgcagggt ttcacgcaca tcggtcatag caaaggcatg tccacgcggc
    43801 ttgttgtaat cgcgcgagaa gggataccct gcccacagga tcaccagccg tggatcttcc
    43861 gccagggcgt caacacgctc tgactgttcc gaggtagctt tccaggagag atattgatcg
    43921 ggatgctgcg gggcaaaggt ttcattcttc gcttccacag ttacaggtgt tgcgggagca
    43981 gccgtttgtt cagcgtgaac agaagtgaaa aagaaaaaag gaatcaataa gctgaagata
    44041 cggcgtgcgt ttatttttat ccttgtcata gggcttcatc cgaattgcgc cattgttgca
    44101 atggcggttt ttattgtttt tcacgacagt aaccgcacct acactgtcat gacattgctc
    44161 gtccctatgt gtaacaaata accacactat gaatgttgtc tttaatcaat tgtaagtgca
    44221 tgtaaaatac cactttagag ttagtcagta tcttcctctt tttcaacagc atgcataact
    44281 gcatgttcct caaagaatta atcaactttt gttgctgacc ttcaaaaatt accctgccgt
    44341 ttatttgcac aattctactt ttgcgtgatc tgtcgcccaa atactaaaca aaactgccaa
    44401 tacccctaca tttaacgctt atgccacata ttattaacat cttacaagga gaacaaaagc
    44461 atgagtcaaa ttcacaaaca caccattcct gccaacatcg cagaccgttg cctgataaac
    44521 cctcagcagt acgaggcgat gtatcaacaa tctattaacg caccagatac cttctggggc
    44581 gaacagggaa aaattctcga ctggatcaca ccgtaccaga aggtgaaaaa cacctccttt
    44641 gcccccggta atgtgtccat taaatggtac gaggacggca cgctgaatct ggcggcaaac
    44701 tgccttgacc gccatctgca agaaaacggc gatcgtaccg ccatcatctg ggaaggcgac
    44761 gacgccagcc agagcaaaca tattagctat aaagagctgc accgcgacgt ctgccgcttc
    44821 gccaataccc tgctcgaact gggcattaaa aaaggtgacg tagttgcgat ttatatgccg
    44881 atggtaccgg aagccgcggt cgcgatgctg gcctgtgccc gcattggcgc ggtacattcg
    44941 gtgattttcg gcggcttctc gccggaagcc gttgccggac gcattatcga ctccagttca
    45001 cgactggtga tcacttccga cgaaggtgtg cgtgccggac gtagtattcc gctgaagaaa
    45061 aacgttgatg acgcgctgaa aaacccgaac gtcaccagcg tagagcatgt ggtggtactg
    45121 aagcgtactg gcggaaaaat tgactggcag gaagggcgcg acctgtggtg gcacgacctg
    45181 gttgagcaag ccagcgctca gcaccaggcg gaagagatga acgccgaaga tccgctgttt
    45241 attctctata cctccggttc taccggtaag ccaaaaggcg tgctgcacac taccggcggt
    45301 tatctggtgt acgcggcgct gacctttaaa tatgtctttg attatcatcc gggcgacatc
    45361 tactggtgca ccgccgatgt gggctgggtt accggacaca gttatttgct gtatggcccg
    45421 ctggcctgcg gcgcgaccac gctgatgttt gaaggcgtac cgaactggcc gacgcctgcc
    45481 cgtatggcgc aggtggtgga caagcatcag gtcaatattc tctataccgc gcccacggcg
    45541 attcgcgcgc tgatggcgga aggcgataaa gcgatcgaag gcaccgaccg ttcgtcgctg
    45601 cgcattctcg gttccgtggg cgagccaatt aacccggaag cgtgggagtg gtactggaaa
    45661 aaaatcggca acgagaaatg cccggtggtc gacacctggt ggcagacaga aactggcggt
    45721 ttcatgatca ccccgctgcc tggcgctacc gagctaaaag ccggttcggc aacacgtccg
    45781 ttcttcggcg tgcaaccggc gctggtcgat aacgaaggtc acccgctgga aggggccacc
    45841 gaaggcagcc tggtgattac tgactcctgg ccgggtcagg cgcgtacgct gtttggcgat
    45901 cacgaacgtt ttgagcagac ctatttctcc accttcaaaa atatgtattt cagcggcgac
    45961 ggcgcgcgtc gcgatgaaga tggctattac tggatcaccg ggcgcgtgga cgacgtgctg
    46021 aacgtctccg gtcaccgcct ggggacggca gagattgagt cggcgctggt ggcgcatccg
    46081 aaaattgccg aagccgctgt ggtcggtatt ccgcacaata ttaaaggtca ggcgatctac
    46141 gcctacgtca cgcttaatca cggggaggaa ccgtcaccag aactgtacgc agaagtccgc
    46201 aactgggtgc gtaaagagat tggcccgctg gcgacgccag acgtgctgca ctggaccgac
    46261 tccctgccta aaacccgctc cggcaaaatt atgcgccgta ttctgcgcaa aattgcggcg
    46321 ggcgatacca gcaacctggg cgatacctcg acgcttgccg atcctggcgt agtcgagaag
    46381 ctgcttgaag agaagcaggc catcgcgatg ccatcgtaac ccacaattgc cggatgcgag
    46441 tcggtaacgg tttgtaggcc tgataagacg cgacagcggc gcatcaggcg ctggttgccg
    46501 gatgcggcgt gaacgcctta tccggcctac attcggcaac ggctgtaggc ctgataagac
    46561 gcgacagcgt cgcatcaggc attgattgcc ggatgcggcg tgaatgcctt atccggccta
    46621 cattcggcaa gggttaccca agcgttaacc ttctcccata agggagcggg aattaaaaca
    46681 atccctacat tacctctgga gaatctgtga tgaatggtac gatttatcag cggatagaag
    46741 acaatgcgca tttcagggag ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga
    46801 ttattatgct ggcagtttat atcggcttta ttttactgat cgccttcgcg cccggctggc
    46861 tgggcacccc gctgaatccg aacaccagcg tcacacgcgg tattccagtc ggtgtcgggg
    46921 taattgtgat ctcctttgtc ctcactggta tctacatctg gcgggcgaac ggcgaattcg
    46981 accgtcttaa taacgaagtc ctgcatgagg tacaagcatc atgaaaagag ttctgacggc
    47041 gcttgccgcc acactccctt tcgcagctaa cgccgcggat gctattagcg gggccgtaga
    47101 gcgtcagcca acgaactggc aggctattat tatgttcctg attttcgttg tgtttacgct
    47161 cggcattacc tactgggcgt caaaacgcgt acgttcgcgt aatgactact acaccgcagg
    47221 cggcaatatc accggttttc agaacgggct ggcgattgcc ggggactata tgtccgccgc
    47281 ctcattcttg gggatctccg cgctggtgtt tacctccggc tatgacggct taatttattc
    47341 gctgggcttc ctggtgggct ggccgatcat tctgttcctg attgccgaac gtctgcgtaa
    47401 cctcggtcgc tacacctttg ccgatgtggc ctcttaccgt ctgaaacaag ggccgattcg
    47461 tattctttcg gcctgtggtt ctctggtggt ggtggcgctt taccttatcg cccagatggt
    47521 gggcgcaggt aaactgatcg agctgctgtt tggccttaac tatcacattg cggtagtgct
    47581 ggtcggcgtg ctgatgatga tgtacgtcct gttcggcggc atgctggcga ccacctgggt
    47641 acaaattatt aaagccgtgc tgttgctgtt cggtgccagc tttatggcct ttatggtgat
    47701 gaaacacgtc ggctttagct tcaacaatct gttcagcgaa gcgatggcgg tacacccgaa
    47761 aggtgtcgac atcatgaagc cgggcgggct ggtgaaagat cctatctccg cgctctcttt
    47821 ggggctagga ctgatgtttg gtacggcggg cttgccgcat attctgatgc gcttctttac
    47881 ggtcagcgat gcccgcgaag cacgtaagag cgtgttctac gccaccggat ttatgggcta
    47941 cttctacatc ctgaccttta ttatcggctt tggcgcgatc atgctggtcg gtgcgaatcc
    48001 ggaatataaa gacgcggcgg gccatctgat tggtggtaac aacatggcag cagttcacct
    48061 ggccaatgcg gtgggcggca acctgttcct cggctttatt tcagcggttg ctttcgccac
    48121 cattcttgcg gtggttgcgg gtctgacgct ggcgggcgca tcggcggtgt ctcacgacct
    48181 gtacgctaac gtcttcaaaa aaggtgcgac cgaacgtgaa gagctgcggg tatcgaaaat
    48241 caccgtactg atcctcggtg tgattgccat tatcctcggc gtgctgtttg agaatcagaa
    48301 catcgccttt atggtggggc tggcgtttgc catcgcggcg agctgtaact tcccgatcat
    48361 tctgctttcc atgtactggt cgaaactgac tacccgtggc gcaatgctgg gcggctggtt
    48421 aggactgatt actgcggtgg tgctgatgat cctcggcccg actatttggg tacagatcct
    48481 cggtcacgaa aaagccatct tcccgtatga atacccggcg ctgttctcta tcagcgtggc
    48541 attcctcggt atctggttct tctcagcaac ggataattca gcggaaggtg cgcgcgagcg
    48601 tgaactgttc cgtgcgcagt ttatccgctc ccagactggc tttggcgttg agcaaggccg
    48661 cgcgcattaa tctctcccct tccccggtcg cttgatcggg gaatttcttc agaacatcat
    48721 cctggccacc agcggcgaaa ccaataccat caatacacct gaaagcatca tgaccaggct
    48781 tgcaactacg ccttcttgtt gacccagttc atacgaccgc gcagtcccgg caccatgaga
    48841 tgccgcgccg aatccagcac ctttcgccat cccttcgcga atagagagac gtaaaaacaa
    48901 catgtcgcca atcgccatgc caaacacacc ggtgaccacc acaaacagcg ccaccagatc
    48961 aggctgtccg ccgagcgact ggctcgctgc caaagcaaat ggcgtagtga ccgaacgcac
    49021 cgccaggcta cgctggatct catctggcag ggtaaacaat cgcgccagcc acactgagct
    49081 ggtcactgcc accaccgttg ccgtcagtac gcctgccgtc aacgacatcc agtgacgctt
    49141 aatgatcgcc cgattgtcat acactggtac cgcaaaggcg atggtggccg gacctaatag
    49201 ccatagtaac cagtgcgctt cgccaatata gttttgccag gaaatattgc caaaaaccag
    49261 cagcataacc agtagcgtag gcgtcagcac cagaggcatc aatggcaatt tacgaaagcg
    49321 gcgataaagg cgtttgttaa gaaaatagat aactagcgta attgccagac agagcatact
    49381 tagctgaaaa ttagccacgg tttaacctgc gcatttcata gcgatagact ttttccacta
    49441 cccaggcagt cgtgcccagc accatcaccg tactcagcgc gatcacagca aaaatacgcc
    49501 agccatccac cagtaatagt tgtgcatagt tcaccacagc caccacggca gggacaaaaa
    49561 acagcaacat ttcggccaat agccaacgcg ccccggctcg aacccattgc agaggaagaa
    49621 tgcgacaaac aatcagcgcc agcatcagga tcatccccac caggttggca ggcagcggca
    49681 aatgcagcca ggagacgaga tactgcgcaa aaataaataa acctgcatac agcacaacct
    49741 gaaaaaatac ctggatagat tgcactacgg caggggtaat gcggcgaagc gccacgggca
    49801 tgagaggata tcctgaaaat aaaacagaat ggtaagtata gagagcgctc gcccctgact
    49861 gaaatgaatt aaaatcattg caggtatagt caaagggaat agttatggat atcagaacgc
    49921 tgcgttattt tgtcgaagtc gtgcgccagc aaagcttcac ccgcgcagcg gagaagttgt
    49981 ttgtcaccca accaactatc agcaagatgc tgaagaatct cgaagatgag ctgaactgta
    50041 tattgttgat ccgcgatggt cgtcggctgc ttctcactga tactggtcgc gtcgtatttg
    50101 agcgcgggct ggctttactg gctgagtttc ggcaactgga agctgagctt agcgatatca
    50161 atcagcttaa aaccggcgtt ttgcggctgg gaatcccgcc gatggttggg atgttgatgg
    50221 ccgggccgat tggccttttt cgccagcgtt accccggcgt agaattgaaa atttcggagt
    50281 ttggtggtct gacggtacaa caagcggtga tgaacggtga actggatatg gcactgacag
    50341 ccctgccagt agaagaaagc ggcctgacaa cgttatcgct gtttagtcat ccactatgtg
    50401 tactgacgcc acattcaggt aaatggcttg aatgtgatgc cgtctcaccg gagacattgg
    50461 cggaacatcc tctacttatc tataacgaag attttgcctt aagtaagcaa ttgatgaagt
    50521 tattcgatga acatggcgtt aagccgagaa ttgcagttcg cagcgggcaa tgggattttc
    50581 tcgccgcgat ggttcaggcc ggtgtcggaa tagccatatt gccggaaccc atttgccagc
    50641 gattagataa aaacacctta aaatggctac cgctggaaag cgaactaagc tggaaattag
    50701 gaatgatttg gcgtgagggc gtttaccttt cccactctgc ccacgcgtgg ctgagctgtt
    50761 gtgaaggata ctggttaacg ccagagtaag tcgggttggc gggctcgccc gccaaccatg
    50821 cagattactg attttcctca atcagcaacg cttcaagcaa atcgagatcg tgcagtaatt
    50881 tttgcagcgt ttcgttactg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt
    50941 cagaacgcaa tgccgccaga cggaagcgac gctccaggtt ctcttccagg attgaacttt
    51001 ctacatcatt acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactaacct
    51061 ctgtgagcag ctggttatcg atattctctt cggtatccgc cgccaaacgc tcctccattt
    51121 tctggatcgc cacaatcgcc acttctgccg ttgccgctcg tgcaatacgt tcctctttca
    51181 attgttgcga atggtccgcg acttctatgt gttgtagcag aataggcaac atcaccacgc
    51241 cgacaaacaa cgagaagaga atgacgccag ccgccaggaa caccagctca tagcgcgccg
    51301 ggaagacgtt accatccggc aagagcagcg ggatagagag cacaccagcc agagtgatcg
    51361 ccccacgcac cccggcgaaa gacgcgatca ggatttctcg tgtggtccac gaaccaaact
    51421 ccatcggctt cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga
    51481 aacggaccag catcagcgcc gcataaatca gaatgatatc ggtaaacagc atccagattt
    51541 cgacgttagg gtcgatttct gccgccatca gcgacgtctc cagaataccc ggcagctgca
    51601 gacctaacag caggaatacc atgccgttaa agacaaattc cagcatcgcc caggtactgt
    51661 ttgcgcgcag gcgcattgcc agcggcgcac ggcgcatcac accggagcgg gtgatggtca
    51721 tcccggcggc aaccgcagcg aggatgccgg aaacgccaat atgttcggca atcagataag
    51781 aagcgaatgg cagcaggaac agcaggacga tctgcgtcgc gggttcatca ccgccccagc
    51841 ggctgaggaa tcgcagcgag cgaccgtaca gccagctcac cacaaaaccg gcgagaatac
    51901 cgccaatggc tactttcata aattcgaccg tcgcaccgcc gacggtgaag atcatcgtcc
    51961 ccatcgccac tgctacggca aacttcaacg acaccaggcc ggaggcgtca ttcatcaacg
    52021 cttcgccctg caaaatgccc atgatttttt tcgggatgcg cccttcgcca acaatccccg
    52081 agagcgccac agcatcggtc ggagaaagca ccgccgccag cgcaaaggcg gggatcagcg
    52141 gaatgcccgg caccacccag taaataagaa agccaatgcc gaccacggtg accaccacca
    52201 gcgccagcgc gagaccgaaa atctctcgtc catgttcgag aaattcacgg gtcggcgttt
    52261 tccagccatc agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa
    52321 actccacatg caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa
    52381 gcggaagcgg gatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga
    52441 gggtcattat cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta
    52501 tgatctgaga aaggcgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac
    52561 acaatgtgcg gagaaaataa aaacgggcag ccattgccac ccgttttcag aatcctatgt
    52621 aagattagat agcccagcca cccgcataga aggtcaccag cgccacggca ataacgaccg
    52681 taccgatgtt caacttgcgc cattcgccgg aaaccagacg accaatcacc agagtcgcga
    52741 agccaatcat gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca
    52801 gacccgccat cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca
    52861 gcaggccaac gtacatcagc gccggagccg ttgcgtaccc cggaacgagg taagagagcg
    52921 gagagaggaa cagaatcagc aggaacagca cgccaacggt gatagccgtc aaaccggttt
    52981 taccgcccgc cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca
    53041 cgaggccaga aaaaacgctg ctcatggagt cggtggtcag tgctttcccg ccgtcgatga
    53101 tctgcccatc tttatccagc aggttcgcct gaccggcgac ggcacggata gttccggtgg
    53161 catcaaatac tgccgtcatc accagtgcca gaacacttgg caggactaca ggattcagcg
    53221 cacccataat gtccaggctg cctatcagtg aattgccgtt ttcatcgctc aatgaaggca
    53281 tggcgaaaac gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac
    53341 cgataatggt cagcagaatg ccaccaggga ctttcagttt ttccaggccg ataatcactg
    53401 ccagccccac caacgacatc atcaccggaa aggtcgtgaa atcccccagc gcgacgggca
    53461 gaccatcaag tgggttttta atcaccagac cgacaccatt agcagcaatc agcagcagga
    53521 acagaccgat accaatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc
    53581 agctacggat acccgtggca gaaattaccg taaacagcac acccatcagg aacacggcac
    53641 ccagcgcgac aggtacgcta atatgttgcc ccagcaccag actgaatgcg gtaaacgccg
    53701 tcagagagat ggcgcaacca atcgccaacg gcagattagc ccacagaccc atcacgatag
    53761 aaccgagtcc ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcccgctt
    53821 tacccaacat gcctggaacg acgataaccg agtagaccat cgccagaaac gttgttaacc
    53881 cggcaaccac ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga
    53941 gtgaaccgcc ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat
    54001 tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag
    54061 gtttgcgaca atttgctgcg tcgcaaaaaa agcaaacgtt tagctccgcg catacaaaac
    54121 gggtggtaaa aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg
    54181 ctgctttttt ctcttttatt gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt
    54241 tatcgtcgat gcgcaagatt gcagcgttat cgagccgtat accgttcagg attgcgataa
    54301 aaaatcgtct gagatgacaa aaatattgcg gctaaaaagt gtacttttac actggcgaag
    54361 aaacgccggt aacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg
    54421 aatgaggtgt gttgacgtcg ggggaaaccc tcctgtgtac cagcgggata gagagaaaga
    54481 caaagaccgg aaaacaaact aaagcgccct tgtggcgctt tagttttgtt catcttccag
    54541 taagcgtgcg ccggtacctt cttctcctaa tcggtcgccc gggttacgca acgggcaatc
    54601 actgcgcgaa aggcagccac aaccaataca tccatccagt tcgtcacgca gcgccactaa
    54661 ggtatgaatg cgccgatcca actcttctcg ccattgggac gaaagctgtt tccactcttt
    54721 cgcacttaac gtatgccctt ctggcaacac gccaaacgct tcacgaatgg ttgcgagcgg
    54781 aatgccaata cgctgagcaa ttttgataat tgcgacatat cgcaacacat cacgtttata
    54841 tcgccgctga ttgccgctat tacggatact ggtaatcaac cctttgcttt catagaaatg
    54901 cagcgccgat accgccacac cgctgcgttt cgccacttcg ccgggggtta gcagcgcttt
    54961 aatgcggggt aatttctttt ccataaatcg ctttacctca agttaacttg aggaattata
    55021 ctccccaaca gatgaattaa cgaactgaac actgaaaaga ggcagattta tgtcccatca
    55081 gaaaattatt caggatctta tcgcatggat tgacgagcat attgaccagc cgcttaacat
    55141 tgatgtcgtc gcaaaaaaat ctggctattc aaagtggtac ttgcaacgga tgttccgcac
    55201 ggtgacgcat cagacgcttg gcgattacat tcgccagcgc cgtctgttac tggccgccgt
    55261 tgagttgcgc accaccgagc gtccgatttt tgatatcgca atggacctgg gttatgtctc
    55321 tcagcagacc ttctcccgcg ttttccgtcg gcagtttgat cgcactccca gcgattatcg
    55381 ccaccgcctg tagttttatt gcccgcgcgt taactcccgc gcgggtaatt accccatcca
    55441 ttgcataaac acctgcggcg gcatcgcctt cgcaaaaaac catccctggc aatagcgcac
    55501 gccgcgtttg cgcagccagt taacctgcgc ctcagtttcg acaccttcag cgatcgtttt
    55561 taaccccagg ctgtgcgcca gctcgatgat gtgttccgca atcaaatgac tggttttgtg
    55621 ggtggtcagc gtctcaacaa acgatttatc gattttcaaa atatcgacat tcaatgattt
    55681 aaggttatgc aagttagagt agccaatacc aaaatcatca attgccactt cgtaaccggc
    55741 cttgcggaaa gccagaataa tcggcgtcat tttgtcgaca tcaagaaatg catgttcagt
    55801 cacttcaaat ttaatttgct gcgggcgcac cgcatgttgc tctgttttct gattggttcg
    55861 cgctatcaac cgtgaggtat gaaaatcgga ggctgacagg ttaatagaaa catagcgatc
    55921 ggtatgtgtt gccagatacg cgcccaaatc acggaagaca ttatcaataa cataatcagt
    55981 tacctgtccg atcatcccct ccttttctgc cagcggaata aactctgccg gattcattac
    56041 ttgcccctgc tcaccaggcc aacgtaacaa cgcttcagcg ccgatacatt tttctgtttt
    56101 gatatcgatt attggctggt aataaaggca aagttgattt ttttcgaggg cgcgttgcaa
    56161 tttgcgtttg ggagacaaat agttttgtcg aatacgtagc cagagtaata gcagaacaag
    56221 actccccaaa ataccggcgg gcaacgcaaa aataagataa ttataaaaat gagttataag
    56281 acgttgatat gaagtcgcaa caatggctgc aattgggcgt tttgtcgaat aaactgtcgc
    56341 atataaatag ccatttcgtt gtacggttaa atcattcaaa tgaatcagcg gagaaaacgt
    56401 tgctgccgag gcctcgttgc ttaacgagaa aaaggttttc gtcaccgtat catacactcc
    56461 ccattgcaat gtcggatcat cagacatcac ttcactccag aagagagggt tgataaccgc
    56521 cacataattt ccccgctgca tgtaggtcat tttatagcca gagaaaaaag gcgtatcacg
    56581 gtaataatag atagaaacgt taggttcgcg cttataatcg gccggtgcaa tcgtatagcc
    56641 atttacagac gctatcagcg atgagcataa aaaatggtta tcacgagcat aaatcaattc
    56701 attaatataa agatagccac gaataatatt caacattcgc ttttgatggg ctggagtgca
    56761 aacttgcccc tgatagcgtt cagctgcatc gctcactaaa tctgcctgac gaatgaccag
    56821 ctcagattta tccaacgcca gctgagcaaa agttcggagc tgattattca cttctgattt
    56881 tgcccaaaag aaggcaatcc atagcgacag aatgatggga aagagaacta aaaagataat
    56941 gcccggaaac gccagtaatt ggtgtcgtgc acgatgactc attgctatca attccctgcc
    57001 tgtttatacc cggttattgc aaggtgaaac cctggctacc gacgtcttta gataaatctt
    57061 acggtataaa tagcactttt gcctgatata aaattgagac ctttttaatc gtcaccagga
    57121 tgacgataat tcattgatct taaaaattaa tcaccttcag gaatgcagag gcggcgggaa
    57181 gatataccct taatgccgct gaaaaaacac ataatctgta cggcaataaa agtcacggcc
    57241 taatctggct aacaaacaac caattgtgat atagttcaca aaattcatga aacaaacaga
    57301 gtgtttcatt tttgtgttct gcaagccccg cgatagccag agtgtatcac gcctcccgtg
    57361 aacaacgccg cgctgtccag ggcatcagct cttttccgag gaattgtttg atggctaccc
    57421 tcaccactgg cgtggttctt cttcgctggc aacttcttag tgccgtaatg atgtttctgg
    57481 ctagcacgct caacatccgt tttcgtcggg ctgattatgt cgggcttgca gtgatcagca
    57541 gcggtctggg cgtagtttcc gcctgctggt tcgcaatggg gttgcttggc atcacaatgg
    57601 cggatatcac cgccatctgg cacaacatcg agtcggtgat gatagaagag atgaatcaga
    57661 caccgccaca atggccaatg attttgactt gatatgtaga agcctccata acggaggctt
    57721 cttttttatg gctggcagat gctttaatca tccaccttaa aacaatataa cctattgttt
    57781 taatgacaaa tcagaacgga atatcgtcat caaagtccat cggcggctcg ttagacggcg
    57841 ctgccggagc ggactgctgc gggcgagact gcgcgccgcc gctgaactga ttgccaccct
    57901 gcggctgctg aggctgaccc caaccgccct gcggctgacc accaccgata ttgccacctg
    57961 caggagcgcc accaccctga cgaccaccca acatctgcat ggtgccgcca acgttcacca
    58021 cgacttctgt ggtatagcga tcctgaccgg attgatcggt ccatttgcgg gtacgcagct
    58081 ggccttcgat ataaacctga gaacctttac gcagatattc gctggccact tctgccagtt
    58141 tgccgaacag cacaacgcgg tgccattcag tctgctcttt catctcgccg gtcgctttat
    58201 cacgccagga ttcggaagta gccagcgtaa tgttggcaac tgcgccacca tttggcatgt
    58261 agcgtacttc cgggtcctga cccagattac caacgagaat aaccttgttt acgcctctgc
    58321 tggccatgtt cgtgtctcct gaaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt
    58381 accattacca atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc
    58441 agacattgtt acacaacact ccgggtaatg cattccaata ctgtatattc attcaggtca
    58501 atttgtgtca taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag
    58561 cgtttaatcc gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat
    58621 ctcaaaaaca tcaacctcgt tatcccccgc gacaagctta ttgtcgtgac cgggctttcg
    58681 ggttctggca aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac
    58741 gttgaatccc tttccgccta cgcgcggcag tttctgtcac tgatggagaa gccggacgtc
    58801 gaccatattg aagggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac
    58861 ccgcgctcta cggtggggac aatcaccgaa atccacgact atttgcgtct gctgtacgct
    58921 cgcgttggtg agccacgctg cccggaccac gatgtaccgc tggcggcgca aaccgtcagt
    58981 cagatggtgg ataacgtgtt gtcccagccg gaaggcaagc gtctgatgct gctcgcgcca
    59041 atcattaaag agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccaaggt
    59101 tacatccgtg ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa
    59161 ttgcaaaaga aacataccat tgaagtggtg gttgatcgct tcaaagtgcg tgacgatctt
    59221 acccaacgtc ttgccgagtc gtttgaaacc gcgctggagc tttccggtgg taccgcggta
    59281 gtggcggata tggacgaccc gaaagcggaa gagctgctgt tctccgccaa ctttgcctgc
    59341 ccgatttgcg gttacagtat gcgcgagctg gaacctcgcc tgttttcatt taacaacccg
    59401 gcgggagcct gcccgacctg tgacggtctt ggcgtacagc aatatttcga tcctgaccgc
    59461 gtgatccaga atccggaatt gtcgctggct ggcggtgcga tccgtggctg ggatcgccgc
    59521 aacttctatt atttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa
    59581 gcgccgtggg gcagcctgag cacgaacgtg cataaagtgg tgttgtacgg ttctggcaaa
    59641 gaaaatattg aattcaaata catgaacgat cgtggcgata cctccattcg tcgtcatccg
    59701 ttcgaaggcg tgctgcacaa tatggagcgc cgttataaag agacggaatc cagtgcggta
    59761 cgtgaagaat tagccaagtt tatcagcaat cgtccgtgcg ccagctgcga aggaacccgt
    59821 ctgcgtcggg aagcacgcca cgtgtatgtc gagaatacgc cgctgcccgc catctccgac
    59881 atgagcatcg gtcatgcgat ggaattcttc aacaatctca aactcgcagg tcagcgggcg
    59941 aagattgcag aaaaaatcct taaagagatc ggcgatcgtc tgaaattcct cgttaacgtc
    60001 ggcctgaatt acctgacact ttcccgctcg gcagagacac tttccggcgg tgaagcccag
    60061 cgtattcgtc tggcgagcca gattggtgca ggcctggttg gcgtgatgta cgtgctggat
    60121 gagccgtcta tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat
    60181 ctgcgcgatc tcggtaatac cgtgattgtg gtggagcacg acgaagacgc gattcgcgcc
    60241 gctgatcatg tgatcgatat cggtccgggt gcgggtgttc acggcggtga agtggtcgcg
    60301 gaaggtccac tggaagcgat tatggcggtg cctgagtcgt tgaccgggca gtacatgagc
    60361 ggtaaacgca agattgaggt gccgaagaag cgcgttccgg caaatccgga aaaagtgctg
    60421 aagctgacag gcgcacgcgg caacaacctg aaagacgtga cgctgacgct gccggtgggt
    60481 ctgtttactt gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca
    60541 ctgttcccga ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat
    60601 cgcgatattc agggactgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt
    60661 ggtcgtactc cgcgttctaa cccggcgacc tataccggcg tgtttacacc tgtgcgcgaa
    60721 ctgtttgcgg gcgtaccgga atcccgtgcg cgcggctata cgccaggacg tttcagcttt
    60781 aacgtccgtg gcggacgctg cgaggcctgt cagggcgacg gcgtgatcaa agtggagatg
    60841 cacttcctgc cggacattta cgtaccgtgc gaccagtgca aaggcaaacg ctataaccgt
    60901 gaaacgctgg agattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc
    60961 gaagaggcgc gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct gcaaacgctg
    61021 atggatgttg gcctgacgta cattcgcctc gggcagtccg caaccacact ttctggtggt
    61081 gaagcccagc gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg
    61141 tatattctcg acgagccgac caccggtctg cacttcgccg atattcagca actgctcgac
    61201 gtactgcata aactgcgcga tcagggcaat accattgtgg taattgagca caatctcgac
    61261 gtgatcaaaa ccgctgactg gattgtcgac ctgggaccgg aaggcggcag tgggggcggc
    61321 gaaatcctcg tctccggtac gccagaaacc gtcgcagagt gcgaagcttc gcatacggcg
    61381 cgcttcctca agccgatgct gtaatcgtta aggccgcttt cagagcggcc tcagattatt
    61441 gacaaagtgc gctttgttct tgccggatgc ggcatgaacg ccttatccgg cctacaaaaa
    61501 catgcaattt caatatattg cagagattac gtaggcctga taaagcgtag cgcatcaggc
    61561 aattttgcgt ttgtcatcat tctcaggccg ctttctgaac ggtcttttcc tttcagagtt
    61621 gcaccagcaa tttacgtttt tcttccggca gcaaattcac cgcctgctga taagacgcat
    61681 ccaccagata atagatttgc gaatccggca gcgaaccgtc gagatacacg gtgctccagt
    61741 gcgctttatt cagatggcgg cttggacgca catcgctgtg ctgctgacgt agcagctccg
    61801 ccagctccgg gctggttttc agcgaaacag ctgggcgatt ttctacttct ttcaccatcg
    61861 caaacagcac atcttccact ttgatctgcg tcgctttcca gtcgttatgc acgctctgtt
    61921 ctgcgcctgg ttttgccatg caatattgta gcaactccga aatggtcatt ttttactccc
    61981 cttgaagtgt cgcgatgatg cgacgcgatc cgccgtggat gcgatgttcc cccagccaga
    62041 tgccttgcca ggtaccggtc tgaatacgcc ctttatgcac cggcaataca agcgatgttc
    62101 ccagcattga ggatttgata tgagaaggca tatcgtctgc tccctcatag tcatgctcat
    62161 aatttccgtt atcgggaacg gcgcggagga aaaaacgctc catgtcgtgg cgtacggtgg
    62221 gatcgcagtt ctcattaagt gtcagagagg cggaggtatg ttgcagcaac agatgcagta
    62281 agccgatgtt aacgcgcggc atatcagcca gctgattcag aatttcatcc gttaccagat
    62341 gaaacccacg agatttggcg ctaagcgtga gcgtcttttg ataccacatg tgctgctcct
    62401 tgataaaact ctcttaatca gtttgcagca agacagcgaa aggataaagg tgtgattaaa
    62461 aaaacagcat tcgggagagt attacgctct cccgctctgt cagtattctg aattgacgat
    62521 cacctcttca ccaaacgcac ccgcttgtgg caagggtttg taggtagagt tggaggcgcg
    62581 cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg gtaatatcgt tatcggaatc
    62641 accataaaaa attcggatat ttttatcctg cagccattgc gattttgtat tttgccctgg
    62701 tttatcgccc gcaaagatca ccggattcat gttggtggca ggaatatgaa aattatccgc
    62761 cagcgttttt gaaaccgttt ctgttttggt cgggctacga ccagtcacaa agaagatcgc
    62821 gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg acctcttttg gaatgctgaa
    62881 ttcatcccag ccattgttca ttttttccca gaacacagga tttttcagat aatcttcgct
    62941 ttctggcgag aaggtttttt tgccgcgcca gaaacccgga ctggaaaaaa gtaccgtatc
    63001 atcgatatca aaccccaccg ccattggcgg acgtcctgcg aggctatttt caatttgtgc
    63061 aaccgaaacc caatgaattg gcgcctgttc agcgagtttg gctacgttag tacccggatt
    63121 aagcggtgaa ggagatgagg ccagggcaac agcggaactg tttagagcga acaataagca
    63181 aacggcactc aatgcctgtg tgatcttgcg catatttttc cctaaatagt cagtaagttg
    63241 aaacttatta ttgtgcgttt gttgaaaaaa ccatctgacc ataacgccaa ccgcggggga
    63301 aaggaaggat tatctgcgga tttgtcacat aaaaagagca tgatcacatt tggaatacct
    63361 gtgcgataaa ccacacattg ccacggttat tctgcttaca cattcacaca atggcactct
    63421 tttggttctc gcaaacattg ttcagggaat atactgatga taaatataaa tgcattcaat
    63481 ggatgatgaa tgaaaatatc gtggaattat atatttaaga acaaatggcg atttcacatt
    63541 acaagcattt cactttttct tatcatgctc gcggtttcaa tcgctttttt gcacttgcgt
    63601 tttaatacct tgtccggtac cgataaaatg cggcttgaaa tgtataagtc cacattatat
    63661 tccaccatcg agcaatttta tgttttaccc tatatgctct caacagacca tatcatccgt
    63721 caggcggtaa ttacgcctga cgatatgacg tccagcgaac tcaatcaacg aattgcacat
    63781 ttcaatactc aactcaaaac cgcagcaata tttattctgg atacccaagg taaggccatc
    63841 gcttctagca actggcagga ccccggcagc tatgtagggc aaaattatag ctatcgcccc
    63901 tattataaac acgccatgtc tggcttaaat ggacgctttt acggtattgg tagcactacg
    63961 aatacaccag gattcttcct ctctacaagt ataaaagata aaggaaaaat tgtcggtgtt
    64021 gtagtagtaa aaataagtct taatgaaatt gaaaaagcat g
//
LOCUS       DAZFRE010000017        60553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFRE010000017 DAZFRE010000000
VERSION     DAZFRE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.017"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            34..705
                     /locus_tag="ACHKUI_001292"
     CDS             34..705
                     /locus_tag="ACHKUI_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6597533.1"
                     /translation="MCTSFFAQSNMTVYPMAVSINSQGEGSVRVISKSNEVQYIKATV
                     FRIDNPSTPLENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAMEPEQQEKNYRVK
                     FEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPVTKLEINSAKKLLNAGNQRL
                     KVLTIAYCKNNSKDNSCKTQTVNKNIFPGQEKSLESISGYDKIVVKYNNWITKDNGEF
                     ELAVH"
     gene            735..1235
                     /locus_tag="ACHKUI_001293"
     CDS             735..1235
                     /locus_tag="ACHKUI_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO6597534.1"
                     /translation="MKKVFVKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NSALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVSWGGEEIKADAATTFSAVKIFASDAITNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1328..4012
                     /locus_tag="ACHKUI_001294"
     CDS             1328..4012
                     /locus_tag="ACHKUI_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO6597535.1"
                     /translation="MDKKLLALLILASLSPAKATLTKIPAGFEVIAQGQQEYIEVYFA
                     GKSLGKYYAMVNLDTVTFLDSSSLYSKLELSTDDQKITHTVKEKLSQPLARHGELACG
                     FVRTDSGCGFLNTETVEIIYNDEESSVTLFLNPQWSSAFNSKSLYLNPDKNTVNAFIH
                     QQDINVLVQDDYQSLSIQGNGALGITDNSYIGAHWNFDGYDADDVSDNNVDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQTQSQQGT
                     PVMILLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGEAGNLAISASVFRNDDGGKGDMQQANWSHPGWPTLGFYRTN
                     SDGDACTTDNRESYNALSCYESISATVSQNLVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTSAQSISETVQLSASRAFVMRDWILSTSLGVFHRNDNGGGSDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRQNHDHLSAFTGTYSSTLAVSRYGINVGA
                     SGTDDLLGAVLVDVKGFSEQDEQSQDLQLEARVAGSRTLQLGQRDSVLFPYPGFQSGF
                     VEVNDSNQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVLKKRASIRYSGDV
                     NCSVVSYSQLPESIQVQAQLKQPKLRGNVQTAQREIAP"
     gene            4009..5091
                     /locus_tag="ACHKUI_001295"
     CDS             4009..5091
                     /locus_tag="ACHKUI_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO6597536.1"
                     /translation="MINRLIAVVLCLFGMVAWVQATPNVTAEITYDLAVGRADYYFWN
                     EEESAGNNAYMWYECSYPNLQQTCTNSGNISTVQIYLTEQRSGMRWPLKLKGFKEAYV
                     MNQVPGCEGYVAKTHLQESNRYNCKATGDPTIYYADKKFLTLYLEQAEMKKLPIGGVW
                     KGQVKLHSNLPAQDYFADITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNASNY
                     AQDLTTLDMCLYDGFNGNAISYEIMLKDEGLPAAGRRDGYFSIYRQGRTTTDDVERID
                     YRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKF
                     SVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5190..6488)
                     /gene="exuT"
                     /locus_tag="ACHKUI_001296"
     CDS             complement(5190..6488)
                     /gene="exuT"
                     /locus_tag="ACHKUI_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6597537.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            6971..8383
                     /gene="uxaC"
                     /locus_tag="ACHKUI_001297"
     CDS             6971..8383
                     /gene="uxaC"
                     /locus_tag="ACHKUI_001297"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6597538.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8398..9885
                     /gene="uxaA"
                     /locus_tag="ACHKUI_001298"
     CDS             8398..9885
                     /gene="uxaA"
                     /locus_tag="ACHKUI_001298"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6597539.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9968..10519
                     /gene="ygjV"
                     /locus_tag="ACHKUI_001299"
                     /pseudo
     CDS             9968..10519
                     /gene="ygjV"
                     /locus_tag="ACHKUI_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
     gene            complement(10523..11767)
                     /gene="sstT"
                     /locus_tag="ACHKUI_001300"
     CDS             complement(10523..11767)
                     /gene="sstT"
                     /locus_tag="ACHKUI_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6597540.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12091..13056)
                     /gene="alx"
                     /locus_tag="ACHKUI_001301"
     CDS             complement(12091..13056)
                     /gene="alx"
                     /locus_tag="ACHKUI_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6597541.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            complement(13339..14325)
                     /gene="ygjR"
                     /locus_tag="ACHKUI_001302"
     CDS             complement(13339..14325)
                     /gene="ygjR"
                     /locus_tag="ACHKUI_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6597542.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGALVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            complement(14404..15096)
                     /gene="ygjQ"
                     /locus_tag="ACHKUI_001303"
     CDS             complement(14404..15096)
                     /gene="ygjQ"
                     /locus_tag="ACHKUI_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO6597543.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            complement(15173..15676)
                     /gene="ygjP"
                     /locus_tag="ACHKUI_001304"
     CDS             complement(15173..15676)
                     /gene="ygjP"
                     /locus_tag="ACHKUI_001304"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6597544.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15761..16897
                     /gene="rlmG"
                     /locus_tag="ACHKUI_001305"
     CDS             15761..16897
                     /gene="rlmG"
                     /locus_tag="ACHKUI_001305"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6597545.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17182..17289
                     /gene="higB"
                     /locus_tag="ACHKUI_001306"
                     /pseudo
     CDS             17182..17289
                     /gene="higB"
                     /locus_tag="ACHKUI_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
     gene            17299..17715
                     /gene="higA"
                     /locus_tag="ACHKUI_001307"
     CDS             17299..17715
                     /gene="higA"
                     /locus_tag="ACHKUI_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6597546.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17808..19826)
                     /gene="fadH"
                     /locus_tag="ACHKUI_001308"
     CDS             complement(17808..19826)
                     /gene="fadH"
                     /locus_tag="ACHKUI_001308"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6597547.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVGAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGEPQILAVDHVVIC
                     AGQEPNRTLAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACHKUI_001309"
     CDS             complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACHKUI_001309"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO6597548.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVIFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKNWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTENGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFQHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACHKUI_001310"
     CDS             complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACHKUI_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6597549.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(23569..25002)
                     /gene="ygjI"
                     /locus_tag="ACHKUI_001311"
     CDS             complement(23569..25002)
                     /gene="ygjI"
                     /locus_tag="ACHKUI_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6597550.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25065..25514)
                     /gene="ebgC"
                     /locus_tag="ACHKUI_001312"
     CDS             complement(25065..25514)
                     /gene="ebgC"
                     /locus_tag="ACHKUI_001312"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6597551.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25511..28603)
                     /gene="ebgA"
                     /locus_tag="ACHKUI_001313"
     CDS             complement(25511..28603)
                     /gene="ebgA"
                     /locus_tag="ACHKUI_001313"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6597552.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28787..29770)
                     /gene="ebgR"
                     /locus_tag="ACHKUI_001314"
     CDS             complement(28787..29770)
                     /gene="ebgR"
                     /locus_tag="ACHKUI_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6597553.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPVLRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGNQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29989..30321
                     /gene="ygjH"
                     /locus_tag="ACHKUI_001315"
     CDS             29989..30321
                     /gene="ygjH"
                     /locus_tag="ACHKUI_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6597554.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(30363..31742)
                     /gene="ygjG"
                     /locus_tag="ACHKUI_001316"
     CDS             complement(30363..31742)
                     /gene="ygjG"
                     /locus_tag="ACHKUI_001316"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6597555.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32160..33680
                     /gene="aer"
                     /locus_tag="ACHKUI_001317"
     CDS             32160..33680
                     /gene="aer"
                     /locus_tag="ACHKUI_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6597556.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKIRGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVACQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33787..34410)
                     /gene="nfeR"
                     /locus_tag="ACHKUI_001318"
                     /gene_synonym="yqjI"
     CDS             complement(33787..34410)
                     /gene="nfeR"
                     /locus_tag="ACHKUI_001318"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6597557.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            34698..35462
                     /gene="nfeF"
                     /locus_tag="ACHKUI_001319"
                     /gene_synonym="yqjH"
     CDS             34698..35462
                     /gene="nfeF"
                     /locus_tag="ACHKUI_001319"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6597558.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELHHELAID
                     FFIHEGGVASSWAMQAQSGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35516..35591)
                     /locus_tag="ACHKUI_001320"
     tRNA            complement(35516..35591)
                     /locus_tag="ACHKUI_001320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35556..35558),aa:Ile,seq:cat)
     gene            35716..36222
                     /gene="mug"
                     /locus_tag="ACHKUI_001321"
     CDS             35716..36222
                     /gene="mug"
                     /locus_tag="ACHKUI_001321"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6597559.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36300..38141)
                     /gene="rpoD"
                     /locus_tag="ACHKUI_001322"
     CDS             complement(36300..38141)
                     /gene="rpoD"
                     /locus_tag="ACHKUI_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6597560.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38336..40081)
                     /gene="dnaG"
                     /locus_tag="ACHKUI_001323"
     CDS             complement(38336..40081)
                     /gene="dnaG"
                     /locus_tag="ACHKUI_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6597561.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40191..40406)
                     /gene="rpsU"
                     /locus_tag="ACHKUI_001324"
     CDS             complement(40191..40406)
                     /gene="rpsU"
                     /locus_tag="ACHKUI_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6597562.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40644..41657
                     /gene="tsaD"
                     /locus_tag="ACHKUI_001325"
     CDS             40644..41657
                     /gene="tsaD"
                     /locus_tag="ACHKUI_001325"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6597563.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLIGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41700..43163)
                     /gene="ttdT"
                     /locus_tag="ACHKUI_001326"
     CDS             complement(41700..43163)
                     /gene="ttdT"
                     /locus_tag="ACHKUI_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6597564.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(43211..43816)
                     /gene="ttdB"
                     /locus_tag="ACHKUI_001327"
     CDS             complement(43211..43816)
                     /gene="ttdB"
                     /locus_tag="ACHKUI_001327"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6597565.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43813..44724)
                     /gene="ttdA"
                     /locus_tag="ACHKUI_001328"
     CDS             complement(43813..44724)
                     /gene="ttdA"
                     /locus_tag="ACHKUI_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6597566.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            44931..45863
                     /gene="ttdR"
                     /locus_tag="ACHKUI_001329"
                     /gene_synonym="dan"
     CDS             44931..45863
                     /gene="ttdR"
                     /locus_tag="ACHKUI_001329"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6597567.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45876..46493)
                     /gene="plsY"
                     /locus_tag="ACHKUI_001330"
     CDS             complement(45876..46493)
                     /gene="plsY"
                     /locus_tag="ACHKUI_001330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6597568.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46598..46966
                     /gene="folB"
                     /locus_tag="ACHKUI_001331"
     CDS             46598..46966
                     /gene="folB"
                     /locus_tag="ACHKUI_001331"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6597569.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47057..47878
                     /gene="bacA"
                     /locus_tag="ACHKUI_001332"
     CDS             47057..47878
                     /gene="bacA"
                     /locus_tag="ACHKUI_001332"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6597570.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48066..49304)
                     /gene="cca"
                     /locus_tag="ACHKUI_001333"
     CDS             complement(48066..49304)
                     /gene="cca"
                     /locus_tag="ACHKUI_001333"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6597571.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(49368..49988)
                     /gene="ygiM"
                     /locus_tag="ACHKUI_001334"
     CDS             complement(49368..49988)
                     /gene="ygiM"
                     /locus_tag="ACHKUI_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6597572.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            50230..51531
                     /gene="ygiF"
                     /locus_tag="ACHKUI_001335"
     CDS             50230..51531
                     /gene="ygiF"
                     /locus_tag="ACHKUI_001335"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6597573.1"
                     /translation="MAQEIELKFIVNHSAVAALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEVALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51554..54394
                     /gene="glnE"
                     /locus_tag="ACHKUI_001336"
     CDS             51554..54394
                     /gene="glnE"
                     /locus_tag="ACHKUI_001336"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6597574.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELTRARLAWAMDFADWPQLTGVLTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            54442..55875
                     /gene="hldE"
                     /locus_tag="ACHKUI_001337"
     CDS             54442..55875
                     /gene="hldE"
                     /locus_tag="ACHKUI_001337"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6597575.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(55995..56054)
                     /gene="ibsE"
                     /locus_tag="ACHKUI_001338"
     CDS             complement(55995..56054)
                     /gene="ibsE"
                     /locus_tag="ACHKUI_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6597576.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56294..57955)
                     /gene="yqiK"
                     /locus_tag="ACHKUI_001339"
     CDS             complement(56294..57955)
                     /gene="yqiK"
                     /locus_tag="ACHKUI_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6597577.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKAIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            complement(57982..58611)
                     /gene="yqiJ"
                     /locus_tag="ACHKUI_001340"
     CDS             complement(57982..58611)
                     /gene="yqiJ"
                     /locus_tag="ACHKUI_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6597578.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            58881..59081
                     /gene="glgS"
                     /locus_tag="ACHKUI_001341"
     CDS             58881..59081
                     /gene="glgS"
                     /locus_tag="ACHKUI_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6597579.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            complement(59116..59415)
                     /gene="ubiK"
                     /locus_tag="ACHKUI_001342"
     CDS             complement(59116..59415)
                     /gene="ubiK"
                     /locus_tag="ACHKUI_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6597580.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPIAELQSPPAIPPVDKAE"
     regulatory      59537..59685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            59789..60442
                     /gene="ribB"
                     /locus_tag="ACHKUI_001343"
     CDS             59789..60442
                     /gene="ribB"
                     /locus_tag="ACHKUI_001343"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6597581.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
BASE COUNT        14618 a        15291 c        16016 g        14628 t
ORIGIN      
        1 aatcgattat tacggctcgg gcctgtctta cttctgtgta catcattttt cgcacaatct
       61 aatatgactg tctaccctat ggctgtttcc attaatagcc agggagaagg aagtgttcgt
      121 gtaatatcta aaagtaacga agttcaatac ataaaagcga cagtattccg catcgataac
      181 ccatcaacgc ctctagaaaa cgaagtggaa attaaatccg gtgatgctaa tcatttggtt
      241 gttatgccac ctaaattcgc cttaccagcc ggtagtagca aaaccgtgcg ctttgtcgca
      301 atggaaccag agcaacaaga aaaaaactat cgtgttaagt ttgaagctgt acccagcatt
      361 gatgatgttg ctaccgataa aaaagatctc tctatgcagt taacggttaa tttaatttgg
      421 ggcatcgttg ttagtgttcc acctcaacaa ccagttacta aattagaaat taattcagca
      481 aaaaaactac tcaatgcagg aaatcagcgt cttaaagtgt tgacaatcgc ttattgtaaa
      541 aataatagca aagataatag ttgtaagact cagaccgtaa ataaaaacat tttccctggt
      601 caggaaaaaa gtttagaaag catatctggc tacgataaaa tcgttgtcaa atataataac
      661 tggatcacca aggacaatgg cgagtttgag ctggcggtcc attaattatc gttgtgataa
      721 acaaaggaat agtcatgaaa aaggtattcg taaaatctct tctggtagcc gcaatgtttt
      781 ccgttgcagg ttctgcatta gcagtacaaa aagatattac tgtcactgct aatgttgatg
      841 ctgctctgga tatgactcag accgataact ctgcgctgcc gaaagcagta gagatgcaat
      901 atctgccggg tcagggtctg cagtcttatc agttaatgac caaaatctgg tctaacgacg
      961 tcactaaaga tgtgaaaatg cagttagttt ctccggcgca actggttcag agcctggacg
     1021 ccagcaaaat tgttccgctg accgtaagct ggggtggtga agagattaaa gctgatgcag
     1081 caaccacttt tagcgcagtt aaaatttttg cgtctgatgc tataaccaac ggttctctgg
     1141 ctaagaacct gatgtttgct cagaccacca aaggtgttct ggaaacaggt atctaccgtg
     1201 gcgtagtcag catttatctg tctcaggata tctaatctgg atatctgaat taacaggtaa
     1261 taaggggggc aagtcgtcct cctttatttt aactattctg gtatttatta tggataacag
     1321 gactgccatg gataaaaaat tactggctct tttgatcctg gcgagtctca gcccggcaaa
     1381 ggcgacatta accaaaattc ccgctggttt tgaggttatt gctcagggac agcaggagta
     1441 tatcgaggtt tattttgcag ggaaaagtct cggtaaatat tatgcgatgg ttaatcttga
     1501 taccgtaacc tttcttgatt catcaagttt atacagcaaa cttgaattga gcactgacga
     1561 tcaaaaaatc actcatacag tgaaagaaaa actatcgcag cccttagctc gccacggtga
     1621 actggcttgt ggttttgtac gtactgattc aggatgcggt tttcttaata ctgagacagt
     1681 agaaataatc tacaatgatg aagaaagttc agtaacgcta tttcttaatc cgcaatggag
     1741 ttctgcgttt aactcgaagt cattgtattt aaatccagac aaaaatacgg ttaacgcttt
     1801 tatacatcag caagacatta atgttctggt acaagatgat taccaatcgt tgtcaattca
     1861 gggtaatggc gcactgggaa taacagataa tagctatatt ggtgcgcact ggaatttcga
     1921 cggttatgat gcagatgatg tcagcgacaa caatgtcgat gtcagcgatc tctattaccg
     1981 ctatgatttt ttacgccgtt attatgtgca ggcggggcgg atggacaacc gcacattatt
     2041 taatgcgcaa ggtggaaact ttacttttaa ctttctgccg cttggtgcaa tcgacggtat
     2101 gcgtatcggg tcgacactca gttatttaaa ccagacgcaa agccagcagg gaacgccggt
     2161 aatgatcctg ctttcgcgca attctcgtgt tgatgcttat cgtaatgaac aactgttggg
     2221 atcgttttac ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccaggcag
     2281 ctacagcgtg gcgttaaaag tttatgaaaa taaccaactc acccgcaccg agcttgtgcc
     2341 gtttaccaaa actggcggtc tgacagacgg aaatgcccag tggttcttac aggcaggtaa
     2401 aaccacatca caggtttctg atgatgaaag ttcagcttat cagctagggg tacgcctgcc
     2461 attacatccg caatatgagc tctacgcagg gctggcgaat gccgatgatg taagtgcttt
     2521 cgagttaggt aataactgga cggcggattt aggcgaggcg gggaatcttg ctatcagtgc
     2581 cagcgtgttc cgtaacgatg acggcggcaa aggcgatatg caacaggcca actggagtca
     2641 tccgggatgg ccgacgttgg gcttttatcg gaccaactct gacggtgatg cttgtacaac
     2701 cgacaacaga gagagctaca acgccttaag ctgttatgaa agtatttccg cgacggtttc
     2761 acagaatctt gtcggctgga atatgatgct gggttatacc cgcacacaaa ataacactga
     2821 tgatagttta cgctgggaca aacagcagag ctttgaaaat aactatcttc gccagacttc
     2881 ggctcaaagc atttccgaga ccgtacaact tagcgcttct cgcgcttttg tgatgcgtga
     2941 ctggattttg agtacttccc ttggggtttt ccatcgtaat gacaacggcg gtggtagcga
     3001 tgacaacggt ttatatctgt cgttttcgtt atctgatacg ccgacgatgg acagcaataa
     3061 caacagccat tcaaccaatg tttctactga ttatcgttat agcgatcagg atggcgatca
     3121 aacgtcatgg cagttatcgc atacctttta taacgactca ttcagccata aagagcttgg
     3181 cgtgaccgtc ggcggtttga acaccgatac cataaacagc gcggttaacg ggcgttggga
     3241 tggtcaatat ggaaatgtct acgctacagt ctcagacagt tatgatcgcc agaatcatga
     3301 tcatctctcg gccttcaccg ggacatacag ctccacgctg gcggtgagtc gctatggcat
     3361 caatgtgggg gccagcggca cagatgattt gctgggggcg gtgttggtgg atgtgaaagg
     3421 tttctctgaa caggatgaac agagtcagga tctgcaactc gaagcgcggg tggctggcag
     3481 cagaacattg cagcttggtc aacgcgacag cgtgttattc ccctatcctg gatttcagtc
     3541 tggctttgtt gaagttaacg acagtaatca gggtaaccag caaggaacaa ccaacatcat
     3601 taacggtgcg ggaaatcgtg agttaatgct gttgccgggc aaattacgtt atcgcgaggt
     3661 gtcggccagt tttaattaca actatattgg tcgcttgtta ttacccgcat cggtagagaa
     3721 gttcccgctg gtggggctga atagcgccat gctactggtg gctgaagatg gcggatttac
     3781 actggagatt aacggtagtg aaaaagaact gtatctgctt tccgggcagc agttcctgaa
     3841 atgtccgctg agtgttttga agaaacgcgc cagcattcgc tatagcgggg atgtgaattg
     3901 tagcgtggtg agttattcgc aactgccgga gtctattcag gttcaggcac agttaaaaca
     3961 gcctaaatta cgtggaaacg tccagacggc gcaaagggag attgcaccat gataaatcga
     4021 ttgattgccg tggttttatg cttatttggc atggtcgcgt gggttcaggc aacacccaat
     4081 gtgacggctg aaattactta tgatttggcc gtcggtaggg ctgattatta cttctggaac
     4141 gaggaagagt cggctgggaa taatgcatat atgtggtatg aatgttcgta ccccaatctc
     4201 cagcaaacct gtacgaattc tggaaatatc tcgacagtac aaatttattt aacggagcaa
     4261 cgtagtggta tgcgctggcc acttaagctc aaaggattta aagaagctta tgtcatgaat
     4321 caagtacctg ggtgcgaagg atatgttgca aaaacgcacc tccaggaatc taatcggtat
     4381 aactgcaagg caactggtga tccaaccata tattatgctg ataaaaagtt tcttacactt
     4441 taccttgaac aggcagaaat gaagaagctg ccaattggtg gtgtctggaa agggcaggtt
     4501 aaactgcatt ctaatcttcc tgctcaggac tattttgcgg atattaccct gaacaccctc
     4561 gatcccaatc atattgacgt attcttcccg gagttcgccc acgctacgcc acgggtacaa
     4621 ctggatttgc acccaacagg cagcgtgaat gccagtaatt atgcccagga tctgacaacg
     4681 ttggatatgt gcctgtacga cggttttaac ggtaatgcca tcagttatga aattatgctt
     4741 aaagatgaag ggctacccgc ggcagggcgc agagatggtt atttttccat ttatcgtcag
     4801 ggaagaacaa cgactgacga tgtagagcgt atcgattaca gagtaaaaat gtataacccg
     4861 gaaacaggtg ggcaaatcga cgtacgcaat aatgaaaata tggtctggaa cagcatcaac
     4921 ctgaagcgag ttcgtccggt ggtattgccg ggcattcgtt acgccgtaat gtgtgtgcct
     4981 acgccactaa ccctggcggt cgataaattc agcgtgatgg ataaacaggc gggatattac
     5041 atgggtaaat tgtcggtcat ttttaccccg tcattgccga cgatcaatta gctctgtagg
     5101 ccagacagac gttttcgcgt cgtctccggt aataaaaacg ctgaagccgc ctgaaaaggc
     5161 ggcttcgtca ttacaactta cattaagtgt taatgttgcg gtgcgggatc gttatgtgat
     5221 tcctgcgcca cctcaatcgc cggtttgttc tgcaacacgg tccagataac cagcgcacct
     5281 aacaggtcga acactgccag aactgcgaac agcgggctga agccgatggt gtcagccagt
     5341 gcaccgacta ccagtgcaaa cagcgtactt gccagccatg cggacatccc ggttaaaccg
     5401 ttcgccgtcg ccacttcgtt acgaccgaac acgtcagacg agagcgtaat cagtgcgcca
     5461 gacagtgcct ggtgagcaaa accaccgata cacagcagca taatcgcgac atacgggttg
     5521 gtgaacagac cgatcatgcc cgggccaatc atcagcacag cgcccagcgt tacgaccatc
     5581 ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca
     5641 ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcaaacat cgcgatctct
     5701 ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc
     5761 catgccggtt ctgccaggaa gcgcggcagc gcgatacccc agaactgacg gttgcgcagg
     5821 atctgaccaa cggacatttt cttcgctgtg tcaacctggt gctgggcttc ctgaccatta
     5881 ataatgtagt cgcgttcttc atcggtcaga tgtttctggt cgcgcggatg cttgtagaaa
     5941 atcagccatg ccatcgccca gataaagctc aacgcaccgg agatgataaa tgccatctgc
     6001 cagctgtgca tcacgattgc ccataccacc agcggcggcg caatcatcgc accaatcgaa
     6061 gaacctacgt taaagtaacc taccgcgatg gaacgctctt tcgccgggaa ccattcggag
     6121 ctggctttca gacccgccgg aatcatcgcg gcttccgcgg caccgaccgc accacgagca
     6181 accgccaggc caccccaact acctgccagc gcagttgcac cacagaacac ggcccacagt
     6241 acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata acccgctacc
     6301 ggttgcatga ccgtataagc agcagaatac gctgcgatga tataggaata ctgttgggtg
     6361 gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg
     6421 taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt
     6481 ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc
     6541 cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca cgccccgaac
     6601 gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga ttcatctaac
     6661 aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg cagaaagata
     6721 acttgtcata caactttaaa aggtgagagc catcacaaat gtgcgaatat ttgtagggac
     6781 attacctgac gacggcgagg ccagtgctgg cgcggcctgc agcgagattt accactttga
     6841 gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc ggactctgga
     6901 aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac tcgttgagag
     6961 gaagacgaaa atgactccgt ttatgactga agatttcctg ttagatactg aatttgcccg
     7021 ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt gccatttgcc
     7081 gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct ggttgaaggg
     7141 cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc tgtgcaccgg
     7201 tgatgcgtct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc acactattgg
     7261 caacccgtta taccactgga cgcacctcga actgcgtcgt ccgtttggta tcactggcaa
     7321 attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca
     7381 ggataatttc tctgcacgcg gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac
     7441 cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagacg gctctttcac
     7501 cattaaagtg ctgccgagct ggcgtccgga caaagccttt aacatcgaac aggcgacctt
     7561 taacgactac atggcgaagc tgggtgaagt ctctgatacc gacatccgcc gctttgctga
     7621 tctgcaaact gccctgacca aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc
     7681 tgaccacgcg ctggatgtgg tgatgtttgc tgaagcgaac gaagcggaac tggacagcat
     7741 cctcgcgcgc cgtctggcag gcgaaaccct gagcgagcac gaagtggcac agttcaaaac
     7801 tgcggtgctg gtgttccttg gtgccgaata cgcacgtcgc ggctgggtac agcagtacca
     7861 cattggcgca ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc cggatgtagg
     7921 ttttgattcc atcaacgacc gtccgatggc ggaagagctg tctaagttgc tgagcaagca
     7981 gaacgaagaa aacctgctgc cgaaaaccat tctctactgc ctgaacccgc gcgataacga
     8041 agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca aaatgcagtt
     8101 cggttccggc tggtggttta acgatcagaa agacggtatg gaacgtcaga tgacccaact
     8161 ggcgcagctc ggtctgctga gccgctttgt cggtatgctg actgacagcc gtagcttcct
     8221 gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgattg gtcgctgggt
     8281 ggaagcgggc gaagcgccgg cggatatcaa cctgctgggc gagatggtga aaaatatttg
     8341 ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg ggttgatatg
     8401 caatacatca agatccatgc gctggataac gtcgcggttg ctttagcgga tttggctgaa
     8461 ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt tgctcgtgga
     8521 cataaatttg cgttaacgga tatcgcaaaa ggggccaacg tcattaaata tggcctgccg
     8581 attggttatg cattggcgga tattgcggcg ggggaacacg ttcacgccca caatacgcgc
     8641 acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga tctgcctgcg
     8701 caagcggcag atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt cggggtgcgc
     8761 aatgagctgt ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccaa
     8821 aatcgtttcc tgaaagagac caacaacgct gaaggtaccg acggcgtgtt cctcttcagc
     8881 cacacctacg gctgctcaca actgggcgac gatcacatca atactcgcac catgctgcaa
     8941 aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac
     9001 aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc
     9061 atgatctgcc aacagcagga tgatgaaatc gaggccggga tcgcgcattt gcatcagctg
     9121 tatagcgtga tgcgcaacga taaacgcgag ccgggcaaac tcagcgaact gaagtttggt
     9181 ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat gctggggcgt
     9241 ttctctgact acgtgattgc taacggcggt actaccgtac tgaccgaagt gccggagatg
     9301 tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg
     9361 gtcactatgg tcaacgactt caaacagtac tttattgccc atgaccagcc gatctatgaa
     9421 aatccatcac cggggaacaa agcgggcggt atcaccacgc tggaagacaa atcacttggc
     9481 tgtacccaga aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg
     9541 aaaacgccgg ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc
     9601 ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgtggcac gccgtatggt
     9661 ggatttgtgc cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac
     9721 tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca gttgctggaa
     9781 gaatttatcg acaccatcgt tgagtttgcc aatggtaaac aaacctgcaa cgagcgtaac
     9841 gacttccgcg aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg
     9901 ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata cctgctaagg
     9961 atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct ttctgattgg
    10021 tatcacgaca tttttcaacc gtgacgaacg tcgcttcaaa aagcaacttt cggtctatag
    10081 cgccgttatt ggcgtacatt tttttcttct gggcacctat cctgccggtg ccagtgccat
    10141 ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg taatggcgat
    10201 ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg tcgaactatt
    10261 gccggtaatc ggcacgattg tcagtacctg ggcgctgttc cgctgtaaag ggctgaccat
    10321 gcgctgcgta atgtggtttt caacgtgttg ctgggtgatt cacaacttct aggcggggtc
    10381 gataggcggc acgatgattg aaggcagttt tctgctgatg aatggcctga atatcattcg
    10441 tttctggcgt atgcagagaa ggggaattga tccgtttaaa gtggagaaaa ccccttccgc
    10501 cgtagacgaa aggggctaag tattagttac gcagggcgct atttgccaga cgatcgtctt
    10561 ccgcctggca agctgccgca gtgaacagca cgtcagttga cgagttcagc gcggtttcac
    10621 aggagtcctg caatacgccg ataataaagc cgacagcaac cacctgcatg gcgatatcgt
    10681 tcgaaatacc gaacatatta caggccagtg ggatcagcaa cagagacccc cccgccacgc
    10741 cggatgcgcc acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat
    10801 caaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    10861 cgcccgccat attgatggtg gctcccagcg gaatagaaac ggaataggta tcgcgatcca
    10921 gattcagctt ttcacacagc gccatattca ccggaatgtt cgccgcagag ctgcgggtga
    10981 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    11041 gaattttcca ccataccagc agtgggttaa ccaccagcgc caccagcagc atacagccaa
    11101 ccagtaccac cagcagttgc gcgtagcccc acagcgtgga gaaaccggtg gttgccaggg
    11161 tagaagaaac cagaccaaaa ataccgatcg gtgcgaagtg aatcaccagt ttcaccataa
    11221 aggtaacggc attcgacata tcgttgacca ggtttttggt ggtttcgtta ccgtgacgca
    11281 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat atagttacct ttcagcagcg
    11341 cgtcaatggg gttggaaacc atgctcatta ccagcccgcg catcacttct acaatgcctg
    11401 acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg aaggcaaaac
    11461 tgaagattac tgcggccaga gcagcagaga aggtgcccag cagatagagg aacaaaatag
    11521 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt accagcatca
    11581 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    11641 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
    11701 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    11761 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
    11821 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    11881 gaagcgaaat atgctggatt ttaaagagaa gtcatatttc gcaacttaat ggttacatct
    11941 gtttgacatg aaaataaagc ccaacgtgcg gcttccagac tgatgacaac gtcaaaaaag
    12001 tactcggtca gtcccctcgc cccttttggg gagagggaga aaacggtgct catgttgatg
    12061 acagagtaca cggcttaggc agattaaaaa ttattctacc cgctgcttat catgccgata
    12121 attcacccag gcgttgataa taaacgtcat caccagaatg ccaaacacca cgcccagcga
    12181 gacagcgatc ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag
    12241 aatcaccgcc agaccatatt tgagcatcga gaaacgctct gccacacccg ccagcaggaa
    12301 atacatcgca cgcaggccga ggatcgcaaa caggtttgag gtcagcacaa taaacggatc
    12361 ggtggtcacg gcgaagatag ccggaatact atccacggcg aaaatcacgt cgctcaactc
    12421 caccagaatc agcaccagca tcagcggcgt ggcatacaac aaaccattct tacgcacaaa
    12481 gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag
    12541 cggcttgtcg ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa
    12601 cagcaggaag gcaccgaaga tatacaagat ccagtcgaac tgtgaaatca gccagctacc
    12661 agtgaagatc atgatggtac gcagaacaat cgccccgagt acgccataca ccagcacgcg
    12721 gcgttgtaat gccgccggaa cagagaaata gctgaacaac atcagccaga caaagacgtt
    12781 atcgaccgcc agcgatttct caatcagata acccgtgaga aaggccagtg cctgtggatc
    12841 ggcaacggcg cgaccttcgg tttgcaccag ataccaccag aaagcggcgt taaacagtaa
    12901 cgacagcgtc acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc
    12961 cccacgacgc ccctgcaaca acaggtcgat agccagcata atggtgacaa caacagcgaa
    13021 tccgccccat agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa
    13081 cggccaacgt cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa
    13141 ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac
    13201 cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt
    13261 gcaggtaaca aagtatgaca gaccattcag tcagtcaatg gcctgttaca taccttttac
    13321 actgttttac tttggcaatt atagttttac gttatctgcc ggaaaaatca ccccagtctg
    13381 gcggcggatc tcagtcagca gtttggcggt gatacgactg accgccagcc ctggatgatt
    13441 caccagatgc tcatccacca gggtagcgaa cagctctgct tcgtagagca tggtattaat
    13501 atgctgtggc tgggtgagat cctgcatctg gctgccacgc ggtacaaagc agactttctg
    13561 gcattcagag agtttttcaa tcaccagcgc tcccgcttcg ccctgaatct cgcttgccag
    13621 gacagaatcg ctcacttttg agtgctgcaa cgtaacgctg aaatcaccgt aatccatcac
    13681 caccacgccg tgggcatcca caccacttgc cagcaaactg gcggttgcct gcacgctttt
    13741 cggttcaccg aataatgcca ccgccgacgc caggcagtaa aagccgatat ccataatcga
    13801 accgttggag aatgccggat tgaaggtgtt gggattctct ccgtccaggt aacgctgata
    13861 ccgcgaggaa tactggcaat agttgaaaaa gactttacgc agtttgccga ctttcggcag
    13921 cgcctggcgt aacagatgaa agttcggcag gtaggccgtt ttaaatgcct caaacagcac
    13981 cacctgattt tcccgcgcac aggcaatggc ggcatccact tccgccagat tcgacgccag
    14041 cggtttctcg caaatcacat gaattttgtg actaaggaaa agttgtgtct gggaaaagtg
    14101 cagggaattc gggctggcaa tatacaccgc gtcaatggca tcgctttctg ccatcgcttc
    14161 cagcgacgta aacagatgct cgacagaaaa atcattggcg aagtgctggg cctgttcaag
    14221 actgcgggaa taaacggcgg ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa
    14281 ctggcgagtg atccagttcg taccaatcac agcgaaacgt atcataacgc gtggatactc
    14341 cataaaggga ttctctcgtc actgtagcac gcctttttta tcttcgacgt gcgcagatac
    14401 gcattattct tttgccggac agccatgctc actaaacggc ccaatcataa ccaacggccc
    14461 gagatatttc ggctggcgat ggaggatttt cgcatcaatc accgcgctga ctctggcaag
    14521 tttttcccgc agttgagtat ataaaccgtg cttcatattc aggtctggcg cgttaaaacc
    14581 gatagcatcg ataccatact gttttgccag ccagatggcg cgttggttat gaaattcttg
    14641 cgagataatg gtgatatggt tttcactaaa caccttgttg gcacgcacca ccgaatcaag
    14701 cgttgagaat ccggcatagt cgcagaagat ggcttttgcc ggtacgcctt tagcgatcaa
    14761 ggcttgctgc attcctgacg cttcatcgta atttttacgt ccattatcgc cgctcaccaa
    14821 tagccatttc acttttccgg cgtggtacaa ctctgctgcg gtatcaatac gtcgggtaaa
    14881 gtagcgatta cccggcctcg cccccagcaa taaacccacg ttacgcgccg gaacggcgtt
    14941 gacatcgctc caggtcagtt gttggctggc gttgaccata acgcgatcgg caataaaaag
    15001 ggttgctcct gctataagca acaataaaca gccaatttta agagtccggc gagaaaaaca
    15061 aatacggaga agaaggcggg caaatgcgcg cagcatggtc gttatcctta acgaaaggga
    15121 ggaggtcgat tttaggcgct aaaggaggaa aaaataacgc ctgacatcat tttcagattt
    15181 tgtcctgccc aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt
    15241 actgcggttc catatgacag cacaactggt aaaacgcttt gttatgctct ttctccttca
    15301 ggtgcgccaa ctcgtgcacc acgatcatgc gcaaaaacgg ttccggcgcg ttgcgaaaca
    15361 cggtagcgac gcggatctct gcttttgctt tcagcttgcc gccctgcaca cgcgaaacag
    15421 cggtatgtaa cccgagcgca tttttcagca catggatctt gttgtcatac atcactttag
    15481 tgatcggcgg ggcattgcgc agaaactgat ttttaagatc ctgagtatat tgccagaggg
    15541 ctttatcggt ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac
    15601 gctgttcgtt aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat
    15661 aagtaagatt gctcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt
    15721 ttcaggggca acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg
    15781 tttccgttca ctgacactac aacgttttcc ggcgacggat gacgttaacc cgctacaggc
    15841 gtgggaagcg gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc
    15901 ggtgttgatc ctgaatgatg cttttggtgc gttaagctgt gtgctggcgg aacataagcc
    15961 ctacagcatt ggtgactcat acatcagcga gctggcgacg cgtgagaatt tacgtctcaa
    16021 cggcattgat gagtcgagcg tgaagttcct cgacagcacc gccgactacc cgcaacagcc
    16081 gggtgtggtg ctgatcaaag tgccgaaaac actggcattg ctggaacaac aactgcgcgc
    16141 gctgcgcaaa gtggtcacgc cagatacgcg cattattgcc ggtgccaaag cccgtgacat
    16201 tcacacttcc acgctggaat tgttcgaaaa agtgctcggt ccgaccacca ccacgctggc
    16261 atggaagaaa gcgcgcctga ttaactgcac cttcaacgaa ccgccgttgg ccgatgcgcc
    16321 gcagaccgtt agctggaagc tggaaggcac tgactggact atccacaacc atgcgaatgt
    16381 tttctcccgc actgggctgg atattggcgc gcgcttcttt atgcaacatc tgccagagaa
    16441 tctcgaaggt gagattgtcg atctcggttg cggtaatggc gttattggcc tgacattgct
    16501 cgataaaaac ccgcaggcga aagtggtgtt tgtcgatgaa tcaccgatgg cggttgcttc
    16561 cagccgtttg aacgttgaaa ccaacatgcc cgaggcgttg gatcgctgcg agtttatgat
    16621 caacaacgcg ctctccggcg tggagccttt ccgttttaat gctgtgctct gcaacccgcc
    16681 gtttcatcag caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accatgcccg
    16741 ccgctgcctg aaaatcaacg gcgagctgta tatcgttgcc aaccgtcacc tggattactt
    16801 ccataaactg aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt
    16861 ggtgctgaaa gcagtgaagc tggggcgtcg tcggtaagat tggctgctac ctgccagata
    16921 aggcgtaaac gccttatctg gcatacgttc agcacattac ccccaggtcg gataagacgc
    16981 ggcaagcgtc gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa
    17041 aaatcccttt caccatcctc tacattctct tgtttagcat ttttctacgt ttattcttcc
    17101 gtcacacaga taaattcatt cgttgccaat ctgtcaacgg atgttatcat attgccaatt
    17161 tgtcaacgga cgtgatgaag aatgcacctg ataactcaaa aagcattgaa agatgctgcg
    17221 gaaaaatacc cgcaacataa aacggagttg gtggctctgg ggaacacgat tgctaaggga
    17281 tatttcaaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagtta
    17341 actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg
    17401 gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt
    17461 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg ctgaatttaa tgccatggcg
    17521 caagccatgc ctggcggtat agctgtgatt cgtaccctta tggatcaata tggtttaacc
    17581 ctttccgatc ttccggaaat tggcagtaaa tcgatggtgt cacgcgtttt gagcgggaag
    17641 aggaaattaa cgctggaaca cgctaaaaaa ctggcaacgc gattcggcat ttctcccgcc
    17701 ttgtttattg attaacgcga cgtgccggat gcggcgtacg attagcacgt tacccccagg
    17761 ccggataaga cgcgttaagc ggcgcattcg gcacttcaat gcacaattta aatctccagc
    17821 gccagccgtg ttccctgggc aattgcccgt cgtgcgtcca gctccatagc tacatcgcag
    17881 ccgccaatta aatgcacagt tttcccgcta tcaatgagtg gttgcgccag cgtgcggttt
    17941 ggctcctgcc ccgcgcagat caccacatga tctaccgcca aaatttgcgg ttcgccgttg
    18001 atcactacat gcagcccgtc atcgtcgatc ttctggtaac ttacgcctgg gatcattttc
    18061 acgccgcgtg agagcagggt ggtgcgatgg atccagccgg tggttttgcc taacccttgt
    18121 cctggtttgc tggctttgcg ctggagcatc acaatctgcc gtgggctacg ggggatttgc
    18181 atgccttgcg gactcaaacc tccggcctgt tgcaggctac tgtcgattcc ccactcatta
    18241 cagaacccgg cgatattctg gctggtggat tctcccggct gacttaaata catcgccgta
    18301 tcaaaaccaa ttccgccaca accgatgatg gcaactttgt tgcccaccgg cgctttgtcg
    18361 cgcagtacat cgagataact caataccttc ggatgatcga tcccgtcgat aggcggaacg
    18421 cgcggcacga tcccactggc gaggatcgtt tcatcgaacg cctgtaactg atccgctgtc
    18481 acggtgtgat tgagttttag cgtcacaccc gtcacttcga tcatccggcg gtaatagcgc
    18541 agcgtttcgt aaaactcctc tttgccgggg atctgtttgg cgatattaaa ctgcccgccg
    18601 atctcgctgt gcgcatcaaa caatgttacc tgatgcccac gcgccgccgc gttaatggca
    18661 aacgccagcc cagcaggtcc cgcaccgacc accgccagat tttttttctg cacggcagga
    18721 aggattggca ttttggtttc atggcaggcg cgaggattca ccaggcacga ggtgactttg
    18781 ccaacgaaga tctgatcgag acaggcctgg ttgcagccaa tacaagtgtt gatctcatcg
    18841 gctcgtcccg attgcgcttt tgacagcagc tccgcatcag cgagaaacgg tcgcgccatc
    18901 gataccatgt ccgcatcacc gcgcgagaga atgtcgtcgg caacctgcgg atcgttaatc
    18961 cggttggtgg tcaccagtgg cagcgaaacg tggcctttca gtttgcgcgt gacccagcta
    19021 aatgcgccgc gcggcacggg cgtggcaatg gtcggaatac gtgcttcatg ccagccaatg
    19081 ccggtgttga taatcgttgc gcccgccgct tcaatggcct gcgccagctc taccgtttcg
    19141 gcaaaagtac cgccgccttc gaccaggtcg agcatcgaca aacggtagat aataatgaag
    19201 tcgttgccga cacgttcgcg caccgcaccc actacttcta cggcaaaccg catccggttg
    19261 cggtaatcgc cgccccactg gtcactacgc tgattggtgc gcagcgtcag aaattcgttg
    19321 atcaaatacc cttcggaccc catcacctct acaccgtcgt atcctgcctc ccgtgccaat
    19381 tgcgcgcagt gggcgaaatc gtcgatcagt tgcaggatct cttcatgggt gagttcgtga
    19441 ggaacgaaac ggttgatggg ggcctgcaat gcggagggtg cgaccagatg cggttggtag
    19501 ctgtagcgcc cggtatgcaa aatttgcagg gcgattttgc cgccttcctg atgtacggct
    19561 tcggtaatgg tgcgatggtg tgggatctgg ttggcgtcat tgagcattgc accgccttcc
    19621 atgccaacgc ctgttaaatc tggtgcgata ccgccgctga caatcaacgc cacgccgtga
    19681 cgggcgcgtt cggcataaaa cgctgccagc cgttcagcgc cgtccgggta ttcctccagc
    19741 ccggtgtgca ttgagcccat caacacgcgg ttttttaacg tggtaaaacc taaatccagc
    19801 ggggcgaaca gcgacgggta gctcataaaa ttgtccagta tgtaaaataa ttgttatgtg
    19861 gtcggatgag ttctaattta gccgtcgccg gagaaaaggg aaaaggggag tgcggggttt
    19921 gtgatgggat tcaaaaagaa tacgcccgtt cgtcatgccg gacggcggta atacagtcgt
    19981 ccggcatcgg gaaaaattac tgcttacgga aaaaatcgtt atacaacata tacaaatgcg
    20041 cggcactcca ggagaaattt ggtgcgcctt gctgtgcgcc agtcagcggg ttgtaattct
    20101 cctgaattgg gccatcggcg gttaaccctt tggcatgctg gaagaacgta tccgccagtt
    20161 tcagggcatc atcgcgataa ccgtaacgct ccattccttt cagaccaaac cagaactgat
    20221 ctacccatac gcgcccgcgc cagtagatat cagcgccaaa agccgggttg gttaacgccg
    20281 ccgttcccag cgggacaaag gtgttgaact cttttggatc gagcatcacc ttcaccaccg
    20341 cgtcggcgtt ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct tctggccctt
    20401 taccgcgctc aacaatcggt ttgcccgcgc agccgttcgc cagtggttta tcttcaatgc
    20461 gcacgtcata gtagaactgt gtagtcgggt cgaacataca ggtgttgatg tagtccgcga
    20521 gctgctgtgc caactggcga tagcgtttgg cctcttccgg tttaccgagg atggttgcca
    20581 tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca
    20641 atagcgagta gcccagcaac gttccgtcct gactgcggtt ctcggcgaat ttcaccgtcc
    20701 agtcgctacg tttgccgccg ttagcgacat atttatccaa ctgttctttg tcgataaacc
    20761 caaagacggc ggcatcatca cgccccgatt cccacgacgc cgcaacctgt gccggaattt
    20821 ccagactgtc gtactggcct ttctccacca cgcgtgcgta gttgttcagc ccagactgcg
    20881 tctcttcttt attgcctttt ttcactgtaa acagcatctc gccattctca gtattgtggg
    20941 ctttatcgcg ggttgcgcca tattccggta cgccgttgcc gttgtgatcg cggttacgta
    21001 gccaccagtc gtgataggcc accagtttcg ggtacatctc tgccagccag tttttatcct
    21061 gggtgacgtt gtacacttcc atcaccgacc aggcggcaag gctgggtttg gtgttacgtt
    21121 cgttccagtt gccgccatcg ccgccacgct ccgggctaag gttccacgct atcaggtcgg
    21181 ggacaaagcc cacatcctgc ggacgcacgc tgtcgcctgg ctggatctgc caggagaaga
    21241 ccgcgcggat attctctttg gcgatatccg gattgaaatg cgccatcgca aacgcctgct
    21301 tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggcg
    21361 tgacggtgtt atatttcacc gcaccgcccg gcgatcgcca gttaccgttg agcgtttcga
    21421 tggctttcac cgcgacgcgc gtctgctccg gcgtcgcatc cggattggtt aaccctttct
    21481 tcagatactc ttcccagcgt tgctgtgagg cagtgagata aaacgccgga cgcgccagaa
    21541 tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga tgggagtagg
    21601 tggtatagag cgtcgtcgaa ccgttgatat gcgccttact ggtaaagcga ttgccgttga
    21661 tttcagtctg cactggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca
    21721 gatcccaggt ggcgcgcact ttgccaaaga tgactttcag gccatcacgg gtggcgctga
    21781 ttttgcgctg atagtcaggg tactcgccag caatggtttt atcggaaagc ggtttccctt
    21841 ctttcgcttc cagtttttcc agcagttcac catcccacac cagatccagc ggtttatcgc
    21901 tggtgatttt ggtttccagt agtgatgtgc gcggcgtggc gaagcgcaga atcatttcga
    21961 cctgtacatc tttcgatatc aacttttgca ccagcgcgcc gggtatactg tatgcctcca
    22021 gcgtgaaatc gactttcttg ccgtcttgcc agacggtcag gcgatcgaaa ttgctggcca
    22081 taaagttgat gtattcttcc gtcagcagcg caacgcctgg aaaaccgccc atggtgttag
    22141 ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct
    22201 ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcacgt
    22261 ttttataatt gtcagcgtta gcggcatggg cggaaaagct catcagcaga gcacaggcta
    22321 ctggcgttaa aatggttttg attttcatga gattactgtc cttattacca gtaaaaatat
    22381 tgcatcagga agagttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta
    22441 tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa
    22501 gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag
    22561 agataaacgc cctttaacgc ttcatacatg gcgctcacca tcatcccgct ttcacttccc
    22621 ggtacgtcca gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttaggg
    22681 gtaagcaggc ccgtgagata gagttggcct gtgccgtcgg tgatttccag accattcatc
    22741 caggtgatac cttctgtaag gtcatactgg atgtaaccgt tcatcattgc cggataggta
    22801 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    22861 tgtccggcag gtgtggcggt aacgctataa cgaaagttgc cgcttaagtt ctgtaccttc
    22921 agcgcagtca taatatcgcg ggtattggga atgacgtagc ctagatcggg cgtgaaatcg
    22981 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    23041 ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa gccaccccag
    23101 ctatggtcag gcacccaggc attaccgtcg tggtcggcct gtacggtcca gccttcgccg
    23161 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    23221 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg
    23281 ccgtaaaaac gaaatgaagg agaagatgtc gaggtcattg cgggggtttt tgagtcgtca
    23341 tgttctgccg gacgtgcttc atcagcggtc acagaaaagg cgtagcaaaa cggcagcgca
    23401 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    23461 aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    23521 ccccatccag gaagagggaa aatgcctgct cattttttat tattctgctt aatcaacatt
    23581 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttact
    23641 gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat
    23701 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc
    23761 aatcagcatt gaaacaacga taataccggt gcggcgggag cccatacgga aatcgcgtgg
    23821 caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag
    23881 catggacgct gcggcagtca tattaataat agtattcatc agatcctgca cggtattgga
    23941 accgagcatc gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg
    24001 cgccggaacg ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat
    24061 ttcggagaag aaaattttca ccggcgttgc cgtccacatc agcagggaac cgaacatcgc
    24121 ggtaaaggac accagaccga caaagcgatt catcagtgct tccggtagac caaaatacgc
    24181 cgccatgccg tggaatacct gtaccgatcc gccagtaaat ttcaactctt tgctgctgac
    24241 gaagacgtta atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag
    24301 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac
    24361 ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt
    24421 aaagtttggg atcatcgcgt caacagtgat ggggtcagca ggctgtacgc cgccaaccag
    24481 tgccgttcct gccagcaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
    24541 ggaggtaatt ggccccagca ttttcgcccc gttggtagaa acccaggtgg agaaggcgaa
    24601 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt agccgaggaa
    24661 ggcatacgaa gcataagcaa taacgcgcgg taacagcgag gtgaaaaaga acaggtttac
    24721 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
    24781 cgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcaa tgatcagaca
    24841 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
    24901 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
    24961 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
    25021 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
    25081 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
    25141 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc tacggtttct
    25201 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac gacctgtaat
    25261 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
    25321 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ccgtctccac gcggtaagtc
    25381 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
    25441 tcaacgcatc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
    25501 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
    25561 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
    25621 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
    25681 agttggagcc gaggccaagc agctggtggt cgagattcag agtgatgtca tcactgcgct
    25741 gaagctcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagcgc caggcgctga
    25801 agttaatggg gcgctgcgga accaccagca gaccgttgcc gtggcgatta gtcagtgccg
    25861 tccagcggac atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg
    25921 catcgacggt actgcgccag atatcaatga tgttagcctg ctggctgtcg gcgtagtttt
    25981 cgcccggtcc acgtccgtaa tatgctacct gatcgtattc gccgttaatt cccatggtga
    26041 aaccgatgca cggaatgatg tgcggatagt cgccataacg ttcgccggaa agcgccacgt
    26101 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcatcccg aagtcaaaca
    26161 ccggtggggc aataaccgtg cggctgacta gcaacacttc atcatcactt tgctcaacga
    26221 caaaatcgcg cagatgctcc tgcatgatct gcaaatgatt tggctgccac agtccttcgt
    26281 actcctgctt atggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
    26341 gcagcgactc gccattcacc tgccaggatg tcggtttacc actcgttttt gagaaggtga
    26401 tcgcaaagtt gtagccgcga acagtacagc tcaaacgatc gtcttccagc gtcagcggac
    26461 gggcgttatt cggtgcgaaa ggtacaggct gcgcggtgtt ttccttcagc gggaactgat
    26521 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
    26581 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgtaag ggggcttcgc
    26641 tgttcggcgc aacgtcgcgc agtttaatct gttgcgtcgc gagcgtttca ccttcggcgc
    26701 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccatagttta ttttcgactt
    26761 tcaactcgcc gcgagtcaga tccagcgcgt ggatttttac cggcgcaata acctgtttgt
    26821 actctttcag gcctggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
    26881 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt gtaccagacg ttgccgttgt
    26941 catcctgcgc ctggatcccg tggtcgcacc attcccagac ataatgtccc tgaatgcaat
    27001 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
    27061 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
    27121 gaggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
    27181 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgccgc atggtacatc gcgcggatgt
    27241 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
    27301 gcgcgtgaat atggcgaaca atgcgctcga cgtaaacttt ttcccactgc ggatcgtcgg
    27361 taatgcggct gatatcgccg acgttagcaa agccgtgcga ttcgacgtcg gtttccgcca
    27421 tcacaaacag gccatagata tcacacagtt cgtaaaaacg tggatcgttc gggtagtgag
    27481 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
    27541 ccatcccaac ggcgcggcct ttgcgatgat cgttgtcgtg gcggttgacg ccgtgcagca
    27601 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
    27661 cgcgttgtgg caccacttcc agaacgttgc cgtcggcgtc tttcagcgtc atgaccagat
    27721 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaaac
    27781 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgtgtt
    27841 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
    27901 gcaccacttc gcaggaaagt gtggcatcgc aataggcttc gtcaaagtcg gtacgaacgg
    27961 tgaaatcgtt aatatgcgtg agttgttttc cgatcagata aacatcgcgg aaaatccccg
    28021 ccgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
    28081 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
    28141 gactgccctt gctgaacccc acatactgac cgttaacgta gacttcaaaa taggtttcga
    28201 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
    28261 gttgataggc accggttggg ttatcgctgg ggacaaacgg cacatcgatg gggaacggaa
    28321 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
    28381 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttctggcact tgcagcggat
    28441 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctcgtttcgc
    28501 gggcaaaggt acgcgcctgc gcaacagaat catatgaaaa aaagtacgca cgcggcgcaa
    28561 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
    28621 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
    28681 tttttactaa aaaatagcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
    28741 cagcactgtc cggcaaaaac gattgccgga cagtgtgtaa ggggatttaa cgggtcgtgc
    28801 cgcgcagttt taatttgctg ggaacgaaga ccaacagcgg cagcgcgcga ccatcgcggg
    28861 ctttttcata caccaggtta acgccctgat ttcccatcat ctcggaatgg atgcgcacgg
    28921 tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa
    28981 tatcctgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg atagcaatgg
    29041 aatcggaagc aacaaacagt gccttcggat agtcttcctg cgccagcatc tgttttgcca
    29101 gttcatagcc cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg cgtaccacgt
    29161 gtttcagacg accatattcc gcaaaggcga cctcacgaat atccgccttg ccgggtaagt
    29221 cttcaccgcc aataaaacca atacgattaa cgccctgatt gatatagaaa tcgatgattt
    29281 ctttactgat acgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa
    29341 agtcgataaa acagatattg tcggtcaatg cgctggcagc ggcgcgcagg acgggcgtgg
    29401 gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaagccg ttgtgttcat
    29461 aacagttggt cagttcgatc gccagctttt cgcactgagt ttcaatgccg tggcggatcg
    29521 ccagatagta aggatcgttg atctccagct cctgctggta gctgtagata gccagaatat
    29581 ggtgttggtt aactgcgcct gtctggagtt tacgggcgct actggtcttg tactccagct
    29641 tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt
    29701 catttaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta
    29761 gtgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg
    29821 cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac
    29881 acagcggctt gcgaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga
    29941 aaattttttg ctatagttta gtaaaatttt actcgaaagg aggaagcaat ggaaaccgtg
    30001 gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc
    30061 catgaaaacg ccgacaagct gtacatcgta caggttgatg tgggggaaaa aacgctacaa
    30121 accgtgacca gtctggtgcc gtactacagc gaagaagagc tgatgggaaa aacagtggtg
    30181 gtcttatgca atctgcaaaa agcgaaaatg cgcggtgaaa cgtcggaatg catgctgttg
    30241 tgtgcggaaa ccgatgacgg cagtgaaagc gtgctgttaa cgccggagcg gatgatgcct
    30301 gcgggcgtac gtatcgtata aaaagatcgg atggcgacgt cgaatcgcca tccgatttga
    30361 tattacgctt cttcgacact gactcgcatg gccgccagcg ccttacgcgc cgctttgatc
    30421 accagttcac actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttagcg
    30481 ttgttgagtg ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag
    30541 ccgatttcgt tatcaacaaa ctcaatcgcc atcaacatcc ctttgccacg cgcttcctgt
    30601 accagatcgg gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt
    30661 tgttcagcct gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc
    30721 gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca ggaacggatt gtcgaacagg
    30781 actgagaaca cctcttcagt ggcgatagtt gcgccaatcg gcatcacgcc accgccgagc
    30841 gctttggcaa ggcagaggat atccggctgt acgttctcat gctcgcaggc gaacatcttg
    30901 cccgtgcgcc ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg
    30961 cacagcttac gcacggcggt gagatagcct ggcggcggca gaattacgcc accttcgccc
    31021 tgaatcggct caaggatcac cgcagcaaca tcatcaccgg tttttttgca ctcgttaaga
    31081 gccgtgcgca tggcttcgat attgccaaac ggtacatgac gaaaccctgg cagcagtggc
    31141 ataaaaggtt tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg
    31201 tggaacgcgc cgctggtggc aataaaagta aacttgccgc gcggtgactg ataagccttc
    31261 gccagcttca gcgccgcttc aacggattcg gtgccgctat tacagaagaa gctgtatttc
    31321 agtttaccgg gcgttagcgc cgcaagggtt ttcgccagca tcgcccgtaa cggatcaagc
    31381 agctcctggc tgtgaagcgg ttgtttcgca agttgattct gtacggcgga aaccacaact
    31441 ggattacggt gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt
    31501 ccctgggtgt cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc
    31561 ccgccggcgg taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag
    31621 tattcaatca cctcccggtt aagtgctttc atctcctcat gatccaacgt tcgcttttca
    31681 atgagattca gggcgtgggc gctgcacgct aaagctgatg cgctcgaagg taacctgttc
    31741 aaaatatgct ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc
    31801 gccactccgg ctgcgatcgc gaaaatcggg ataaacacaa atgtggttat catttgcgca
    31861 atatttaatg ctatttgcta acatttgctg ttattttgca cggtttttgt cgggtatgga
    31921 tttgatgatt gctgcgcaaa tgcacgcttt cagggcaata attgcctgat ttttgggcaa
    31981 ggtaattaac gttttcgtat agtaacagag tgaaagtatt ttaaatcaag gaattagatt
    32041 tgtgctttag tcgcaaattg cgatctaaat caaattaatt ggttaaagat aaccgcagcg
    32101 gggccgacat aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca
    32161 tgtcttctca tccgtatgtc acccagcaaa ataccccgct ggcggacgac accactctga
    32221 tgtccactac cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga
    32281 gcggctatac cttgcaggag ttacaggggc aaccgcacaa catggtgcgt cacccggata
    32341 tgccaaaagc ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg
    32401 gcatcgtgaa aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac
    32461 cgatggtgcg cgagggaaaa atccgtggct atatgtcgat tcgtacccgg gcgacggatg
    32521 aagagatcgc ggcggtggag ccgctgtaca aagcgttgaa cgccggacgg accagtaagc
    32581 gtattcataa aggcctggtg gtgcgtaaag gttggttggg aaaactgcct tcattaccgc
    32641 ttcgctggcg ggcgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgctct
    32701 ggtttgttgc tgccccggtg gtgacgtata tcctctgtgc attagtggta ttgttggcaa
    32761 gcgcttgttt tgaatggcag attgtccgcc cgatagaaaa tgtcgcctgt caggcactga
    32821 aggtggcgac cggagagcgt aatagtgttg agcatctgaa gcgcagcgat gagctggggc
    32881 tgacattacg tgcggtaggg cagcttggcc tgatgtgccg ttggctaatt aatgatgtct
    32941 caagccaggt gtccagtgtc agaaacggca gtgagacgct ggcgaaaggc accgatgaac
    33001 tgaacgaaca tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc
    33061 aaatggcggc gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta
    33121 tcaccgccag taatgcggca gtgcagggcg gggaggcgat gactacggtg atcaagacaa
    33181 tggacgatat tgccgacagt acccagcgca ttggcaccat tacttcgctg attaacgata
    33241 ttgcgtttca gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg
    33301 aacagggcaa aggttttgca gtggtggcgg gggaagtgcg tcatttagcc agccgcagcg
    33361 ccaatgctgc caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatctg
    33421 gttcgcagca ggtacacgcc gccggacgga cgatggaaga tattgtggcg caggtaaaaa
    33481 acgtcaccca gttgatagct cagattagcc attcaacgct ggaacaggcc gatgggcttt
    33541 ccagcctgac ccgtgcagtg gatgagctta acctgatcac ccagaaaaac gccgagttgg
    33601 tggaagagag tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ttggaagacg
    33661 cggtgacggt gctgcattaa tcggtatgcc ggatccagcg gttggagcac aatgcctgat
    33721 gcgatgctgg cgcatcttat caggcctacg gggtgtgtag ggtacgctgc atccggcaat
    33781 cggtgattaa tccagttggg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    33841 ttttatttgt gcatcagtga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    33901 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gcagcgccaa cgcaacgcgc
    33961 tttgatgcgt tcttcaatca tctctacctg ttcgcggttt tcttccagcc actgcgcgcc
    34021 ttgttcggtc agcgcaatct gcttcttacc accttcctct tcgcgaatgg taatcagcga
    34081 ctgctcctgc agaaaatcca gcgttgggta gattacgccc gggcttgggg tgtaattccc
    34141 ctgggtcaga ttttcaatcg ctttaattaa ctcgtaaccg tggctgtcat cgcgcgagag
    34201 aatatccaga atcaccagac gcaattcacc gtgaccaaag aaacgttgtc gacgaccgcc
    34261 gccgcgaccg tggcgaccac cgcagcattg accgtgttca tgctggtgac cgtgtccgca
    34321 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    34381 tttacagcac ccttcgtgat gatggctcat atcagcctcc ctttgcttaa ttagatatat
    34441 ctaatttata tctgatataa gcattactgc aagtacaaaa atgattttta aacgcaggtt
    34501 tactcaacat gtaggccgga taaggcgttc acgccgcatc cggcattgag caatgatgct
    34561 tgataagagg ttgcgcgtct tatcagacta cagatatatc agtgctacaa atcacttgca
    34621 tttatcatga ttaacaatca ttatcatttg tggaatttat ttagatatat ctgattacca
    34681 tcacgaaggc gataacaatg aataacaccc cccgctaccc acagcgcgtt cgcaatgatc
    34741 tgcgcttccg tgaactgact gtgttacgcg ttgagcgcat cagcgccggt tttcagcgca
    34801 ttgtcctcgg cggcgaggcg ctggacggtt ttacatcgcg cggctttgac gatcacagca
    34861 aactcttctt tcctcaacct gacgctcact ttgtgccgcc aacggtaacg gaagagggca
    34921 tcgtctggcc ggaaggacca cgcccaccgt cgcgtgacta tacgccgctg tatgacgaac
    34981 tacaccatga actggcgatt gatttcttta ttcacgaagg tggcgtcgcc agcagctggg
    35041 caatgcaggc gcaatcgggc gataaactca cggtggcagg tccgcgcggt tcgctggtgg
    35101 tgccagagga ttacgcgtat cagctgtatg tctgcgatga atccggaatg cccgcactgc
    35161 gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc gcaagttagc gcgctggtta
    35221 gcgtgcggga taacgcctgt caggattatc tcgcgcacct tgatggtttt aatatcgaat
    35281 ggctggcaca tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa atccctgccg
    35341 atgattactt catctggata accggcgaag gcaaagtcgt taagaattta agccgtcgct
    35401 ttgaagcgga acagtatgac ccacagcggg tacgcgcggc ggcttactgg cacgcaaagt
    35461 aactgatata taaaaagaaa aaggctgacg atttctcgtc agcctttgct atgtctggtg
    35521 gcccctgctg gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga
    35581 gctaaggggc catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg
    35641 cgcgcctgct gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt
    35701 tagaaaggag taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc
    35761 ggtatcaacc ctggtctttc atccgccgga acgggttttc cctttgctca tccggcaaac
    35821 cgcttctgga aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag
    35881 gcacagcatc tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg
    35941 caagccaatg aagtgtcaaa gcaggagctg cacgcaggcg ggcgtaagct aattgaaaaa
    36001 atagaagatt atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga
    36061 ttcagccagc gcggtgcaca gtgggggaaa caaactctca ccattggttc gacgcagatt
    36121 tgggtcctgc caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg
    36181 tatcgcgagc tggaccaggc gctggtggtg cgtgggcgat aaaaacgcca ccgaaaaggt
    36241 ggcgtttgtg cggagggact agtgccgggt gcggcgtaac acctgatccg gcctaccgat
    36301 taatcgtcca ggaagctacg cagcacttca gaacggctcg ggtgacgcag tttgcgcagc
    36361 gccttcgctt cgatctgacg gatacgttcg cgggtaacgt cgaactgttt acccacttct
    36421 tccagcgtgt ggtcggtgtt catatcgata ccgaaacgca tacgcagaac tttcgcttca
    36481 cgcgcggtca ggccagccag cacgtcgtgc gttgccgcac gcaggctttc ggtggtcgca
    36541 gaatccagcg gcagctcgag ggtggtatcc tcgatgaaat cccccagatg cgaatcttca
    36601 tcatcaccga tcggcgtttc catggagatt ggctctttgg cgatcttcag cactttgcgg
    36661 atcttgtctt ccggcatcag catgcgttca gccagttctt ccggcgttgg ttcacggccc
    36721 atctcttgca gcatctggcg agaaatacgg ttgagcttgt tgatagtctc aatcatatgc
    36781 accggaatac ggatggtgcg cgcctgatcc gcgatagagc gggtgatcgc ctgacggatc
    36841 caccaggtag cgtaggtgga gaacttgtaa ccacggcggt attcgaattt atcaaccgct
    36901 ttcatcaggc cgatgttacc ttcctgaatc aggtcaagga actgcaagcc acggttggtg
    36961 tatttcttgg cgatagaaat aaccagacgt aagttcgctt caaccatctc tttcttcgca
    37021 cggcgggctt tcgcttcacc gatggacata cgacggttga tatctttaac ctgctcgatg
    37081 gtcaggccgg tttcttcttc aatctgctgc agtttctgca gggcgcgatg cacttcttca
    37141 gagacatcgt gcagtttttc cgaccacggc ttgttcatcg caattgccgc gttgaaccag
    37201 gtatcgctgg tttcgttgcc ggtaaacagg gtaatgaagt ttttcttcgg cattttgcac
    37261 tgctcaacgc agagcttcat gatcagacgt tcttgcgtac gaacgcggtc catcatgacg
    37321 cgcatgctgt tgaccaggta gtcaaactgc ttcggcacca ggcggaactg tttgaatact
    37381 tcagacagtt tcaggatctc ttcctgagcg gcagcgtgac tgcggccttt cgctttgatg
    37441 gtgtcacgcg ttacaatgta ctgagcgcgc agttccgcaa atttttcgcg agccagttcc
    37501 gggtcgatgc tgttgtcatc atcggcggta tcgtcgtcac catcttcttc gtcttcatct
    37561 tcgtcatcgt ccagatcttc ctgggaaagc tcagaaccga cgtgagtggc ggtaggtgcc
    37621 aaatcttctt ctgcgttcgg gtcaacaaag ccggtgatca gatcggacag acgcgcttct
    37681 tctgcttcaa cacgatcgta ctgttccagc agataggtga tcgcttccgg atattcagca
    37741 acggagcatt gaacctggtt gatcccgtct tcaatacgct tagcgatgtc aatttcgcct
    37801 tcgcgggtca acagttcaac ggtgcccatt tcacgcatgt acatgcgtac cgggtcagtc
    37861 gtgcgcccga tttcagattc cacgctggaa agcacctgcg cggcggcttc ggcagcatct
    37921 tcgtccgcgg tgttttcagc cagcatcaga tcatcggcat ccggtgcttc ttccatcacc
    37981 tgaatgccca tgtcgttgat catttggatg atgtcttcga tctgatctga atcgacgata
    38041 tcttccggca gatggtcatt gacctcggca taggtcagat agccttgctc cttaccacgg
    38101 gtgacaagaa gtttcagctg tgactgcggg ttttgctcca taagacggta tccacactta
    38161 tttcatgagg ttggtgttgg gcgattgacc cgatgtcctt cagctgttaa cgctgaaatc
    38221 caacggttgt ttaccgccaa cgataattac gagggcgtac ttatatattt gccgctgcct
    38281 ctcagtgcgg ctgtcggggg cttcccgatc gctcttcggc acttaagccg ttaaatcact
    38341 ttttcgccag ctcctggttt aatgtccaga gctccaggcg ttcttcgttg cttaaaccat
    38401 gcgtgcgctc acgagcgatt aactcttcct ggcgcagttc aagcagcgaa tcaaacatat
    38461 ggttgagtga gtcggtgaag gtttgctcag caatattcct atctgctata tcgtcccaca
    38521 tcgacagttt ttcaagggtg gcagcattat ttgtaccacg atagtgctct aaaagttgcc
    38581 cggtggtcag acctggctgg gagagacaag tgttgaccag ttctctgaat aagccaagcc
    38641 cagggagctt attttcatcc agattctcaa gcggcgggac caacgtcgct aattctggat
    38701 tttgcaccag caaccctata agtatacgca tggtcgtgcg ttttagctgc ggaacagggc
    38761 gagaaacgcc gctctctgcc gcttttggca ttaatcgttc aagctggctg tcatcaagta
    38821 tgcctaattt gttgcctaat tcctgacgta gatatattcg cagcgtttcg cccggcactt
    38881 gcgatatcag tggcagtgcc agcgtactca aacgtgcgcg cccgtcaggg gtactcagat
    38941 caacttgcgg catcagactg ttaaacagaa atgcggagag tggcatcgcc tgctccatcc
    39001 gcgcttcaaa cgcttcttta ccttcttttc gtaccagcgt gtcaggatct tcgccatcag
    39061 gcaaaaacat aaagcgtagc tgacgaccgt ctgtcatgta aggcagcgcc gtttccagcg
    39121 ctcgccaggc ggcatcgcgg cctgcacggt cgccgtcata acagcaaatg acattgttgg
    39181 tcgcgcggaa caacagttgt atgtgatcgg cggtggttga cgtacctaac gacgcaacgg
    39241 cgtaattaat gccgtattgc gccagcgcca ccacgtccat atagccttcg accacaagca
    39301 gacgattggg ttcagcgtta tcctgctgcg cttcataaag accgtaaagc tggcggcctt
    39361 tatggaaaat gtccgtttcc ggcgagttca ggtatttggg agtatcgttg cccagcacgc
    39421 gcccgccaaa accaatcacc cgaccgcgtt tatcgcgaat ggggaacatc acccgctcgc
    39481 ggaaacgatc gtaactgcgc ccctgatcgt tagtgaccaa catgcccgca tcaatcaatg
    39541 actggcgatt ttctggattg ccgccaaacc gcttcagaac gttgtcccag ccggggggcg
    39601 caaaaccaat cgcaaagcgg gcgataacct cgtggcttaa tccgcgtttt tccagatact
    39661 ggcgcgcaga cgtggcaaca ggttgttgta aagattgttg gtaaaacgta ttcagaccgt
    39721 ccatcaactg ataaagcgtt tgccgctgat ggcgttcgat ctggctgggg ccgctgcctg
    39781 cttcaaatgg cacttcaaga ttgtgcattg ctgccagctc ttcgaccgtt tcgacgaact
    39841 cgagcttgtc gtagttcatc aggaagtcga tcgcgttgcc atgcgcgcca catccaaagc
    39901 agtggtaaaa ctgtttctca ccgttaacgg tgaatgacgg ggttttctcg ttgtggaatg
    39961 gacaacacgc gtggaaattc ttgccctgct ttttcagctt cacacgggca tcgatcagat
    40021 cgacgatgtc agtgcgtgcc agcagatcat taatgaatac gcgtgggatt cgtccagcca
    40081 taggccccga tttttagcaa ttcataaacg aaaataagcc gcgcattcct ttcggaagca
    40141 cggccttaca actacaactc ggtctgatcg gagagcaacg ctctcgggaa ttagtacaga
    40201 cgagtgcggc gtgcgttttc gcgagccagt ttcttcgcgt gacgtttcac tgcagaagct
    40261 ttagcgcgct tacgttcggt agtcggtttt tcatagaact cacgacgacg aacttccgcc
    40321 agaacacctg ctttttcgca ggaacgcttg aagcgacgca gagctacgtc gaacggctcg
    40381 ttttcacgta ctttaattac cggcatgtgc ctctcacctt tgattaattc ggtttgccgc
    40441 tggcatcaac gccagcttat ttcaaaatgg tgcggaattt tactgcaatt gctgctgctt
    40501 tgtaaagcac cgcggccttt tttgaaaggg acttttataa gggtgaggag tatacacgag
    40561 gctttctcca ggggcgaaca aagttttaca tcaacccgca ttggtcctac actgcgcggt
    40621 aataaagcga ggtaaaacaa gtcatgcgtg tactgggtat tgaaacttcc tgcgatgaaa
    40681 ccggcatcgc catttacgac gatgaaaaag gtttgttagc caaccaattg tatagtcagg
    40741 tgaaattgca cgctgactac ggcggcgtcg tgcctgaact ggcctcccgc gatcacgtgc
    40801 gtaaaaccgt accgttgatc caggcggcgc taaaggagtc aggtttaacg gcaaaagaca
    40861 ttgatgctgt ggcctatacc gcaggccctg gattagtcgg cgcgctgctg attggcgcga
    40921 ccgtggggcg ttctctggcg tttgcctggg acgttccggc gatcccggta caccatatgg
    40981 aagggcatct gttagcgccg atgctggaag ataacccacc ggaatttccg tttgtcgcgc
    41041 tgctggtttc cggcggtcat acgcagttaa tcagcgtgac tggcattggt cagtacgagc
    41101 tgctcggcga gtctatcgat gatgcggctg gtgaagcgtt tgataaaacc gcgaagctgc
    41161 tggggctgga ttatcctggc gggccattac tgtcgaaaat ggcggctcag ggtactgccg
    41221 ggcgctttgt cttcccgcgc ccgatgaccg accgtccggg gctggatttc agcttttctg
    41281 gtctgaaaac cttcgcggca aataccattc gtgataacgg caccgacgac cagacgcgtg
    41341 ctgatatcgc ccgcgccttt gaagatgcgg tggtcgatac gttgatgatt aagtgtaagc
    41401 gagcgctgga tcagacgggc tttaagcgac tggtaatggc aggtggcgtg agtgctaacc
    41461 gcacgttacg ggcgaagctg gctgaaatga tgaaaaaacg ccgtggcgaa gtcttctacg
    41521 cgcgtccgga attttgtact gataacggcg cgatgatcgc ctatgccgga atggtgcggt
    41581 ttaaagcagg cgcgacggcg gatcttggcg ttagcgtgcg tccgcgctgg ccgctggcag
    41641 agttaccggc cgcgtaaaac tattaggtgc cggagagcaa tttccggcac cgtcctcact
    41701 taaagcaaca ccatgggcat ccacagtaag ccggtaatca ccagcaacac gaggaatatc
    41761 agcccaaaaa ttgcccccag tcgccagtaa tccaccgttg gcagataacc actaccgtag
    41821 taaatcggac tgggtccggt ggcgtatggt gtaagaatgc tccccagacc aattgccgca
    41881 ccaaccatca ggcagaacac cggcagcggg atttccggca tcgccagcgc ggcggcaatc
    41941 atcatcggtg cgagagcgga ggtatacgct gtggcgctgg caaaaaagta gcgcagcaga
    42001 taaaagacca caatcaacgc caccatcact atcgttggcg aataaccgct taagctgcct
    42061 gctaacagtt tgccaaacca gctaataaaa ccggtgttgt tgagtccggt agcgagggtg
    42121 ataagcgagg ccagccagaa gaaaacgttc cacgccgctt tattactgac aatgtcgtcc
    42181 cagcagataa tgcgcagaag cagcatcagc gccaccacgc tgtaaccgac catcgcggca
    42241 tcgatataat caccgccaaa aatccacagc accagtgcgc ctaccatcag ccccagcatc
    42301 cgtttttcac gcgaacagag cgggcccatt gcctgcagtt ccgtctctgc ccagcgcggc
    42361 acctgatcgc cagacttcag tatcggcggg tacagcacgt aagccagcca gggaaccagc
    42421 agaaccagta aaatactgag cggcaacatc ccgaggaacc agtcgcccca gctcagcgtg
    42481 gcgttagatg cgcttttcat cagtccaatt aacagcaaat ttggagccat cgccgtcagg
    42541 aaaatggcgc tggtcacgca atcggcaaca atccccatcc acatgatgta cgagccaatg
    42601 ctgcgcgaac tgctgtcgtt tggttgtgat tgatagagcg gtggcaggtt acggatgatg
    42661 ggatagataa tccccgcacc acgcgccgag ttggacggtg ttacaggcgc gagtatcagc
    42721 tcggagaaca tcactgcata gccgagaaac agcgtgcgat gccccatttt tttcaccaga
    42781 atcagagcga tgcgacgtcc aagcccggtt ttttcatagc ccgtaccaaa cataaaggcg
    42841 gcgaaaataa gccagataac tgaattagaa aaaccagaaa ccgcccagga gagggatttg
    42901 gcggtgaact taaaacctgg ctgggcgagc tgctccgggc taaacagcag ccacggcgaa
    42961 agaatggcga tgatggatat acccaccatc gccaccacgg cacccggcac gggttcgagg
    43021 atcagtccaa cgatcacgcc agtaaaaacg gcaaagtaga gccaggtatg actctccagc
    43081 cctgcgggaa gcggaagtag agcaataatg gcgatgaccg ccagcggcgc aaggtatcgc
    43141 caccattcag tggaaggttt catcgtgtta tgcctgagaa aaaggagagg ggcgagcctc
    43201 tccgggaggg ttatttgatg tagtggacgt gctcgcagat ctcttctacg atgggatcgc
    43261 ggcgttcggc gaacagcttt ttgttttcgg ctatcaggtt gttgccgtgg gtatctatag
    43321 agacaatcag cgggccaaac tctttgaccc ggcagaccca cagcgactct ggcatcccga
    43381 gttctgtcca gtgtacctct tcaatctctt ccacttgagt tgccgccagt accgcacagc
    43441 cagccgggaa aatcacatgc agcgccttga atttctggca gccttcttcg gtcagcggcc
    43501 ccattccgcc tttgccgacc accagtttca cgccggtctg ctcgataaat tcacgttcaa
    43561 aactttccat acgcatactg gttgtcgggc cgacggacac catctcccat ttgtcgccgt
    43621 ttttgcgcac gatggggcca gcgtggaaaa tcgctttgcc gttgagatcg taagggatcg
    43681 gacgtttcag ttcgatcaat cggcggtgac aaacgtcacg gcaggtcacc agcgtaccgg
    43741 tcaggtagat cacatcgccg acgcgaatat cttgcagatc ttcagctttg atcggggttg
    43801 tcaggatctt tttcataacg cgctccgggt atgagacaga ttttcaaagg tgagatcggc
    43861 atgaactcgc agtgtgccgc gacgatgcgc ccagcagccg gtagaaacag caacgccgat
    43921 ggttgacgga tggcgagcgg cagattcgat atgcacgccc atcactgaac tgttgccagt
    43981 cagcccttgt ggaccaatcc ccagacggtt gagtccttct tccaggcgta gctccagctc
    44041 tgccgctttt ggattgggat ggcggctgcc aatcgggcgc aaaatggctt tacgcgagag
    44101 cactgcggcg gtttccaccg aggtagcgat gccaacgccc acaagtaccg gcggacaggc
    44161 gtttacggcg agggtggaga tattttcgaa gacgaatttc actacgcctt cgtagccttc
    44221 tgacggcatt aacactttcg atcggccagg tagcgtgcag ccgccgcctg ccatgtaaac
    44281 ttcgatttcc gcatcgtcac cgtcggggac gatatcccag gtgacccacg gtacgccact
    44341 gccggtattt ttgccggtgt ttacttcgtc aaaaatttct accgcattgt gacgcagtgg
    44401 cgctttgacg gttgcctctt ccacggcttg tttgagtatg ctttgcagct cgccaagcag
    44461 tgggaaacgg gaaccgacct taacaaaaaa catgatttcg ccggtgtcct gacaggcagg
    44521 acgattcagg tcgatcgctt tttgcatgtt atcgaacatc gtgtggtaga tgatttttcc
    44581 catcgacgat gtttcggcat cctttagctg ttttagttta tccaccacgt catcaggcat
    44641 tcgggtagaa atcatggcgg taaagttagc gacaatctcc gtcaacttat tcactgcctg
    44701 ttgcttatta ctttcgctca tcatggcaac tccagtttat gtatttgtct ggaaaaattc
    44761 accgccacaa actaccacta aaaatgtggt tttatatttt ttgccaccgt gaatatgtaa
    44821 ttcacagttc gtgaatcaac gcgaaaataa gaacgaatat tgcgccagag cagggaatgg
    44881 taatgttttg cgaaatacac tgcgtttatc aaaactgcgg atatttaaca atgctaaata
    44941 gctggccttt agccaaagac ttgcaggtac tggtggaaat cgtccatagc gggagtttta
    45001 gtgctgcagc ggcgacgctt gggcaaacgc ctgcatttgt caccaagcgg atccaaatcc
    45061 ttgaaaatac gctggcaaca actctgctga accgctccgc tcgcggcgtg gcgctgactg
    45121 aaagtggcca gcgttgttac gaacatgcgc tggaaatcct cacccaatat cagcgactgg
    45181 tcgatgacgt cacgcaaatc aaaacgcgcc cggaggggat gatccgcatt ggttgcagtt
    45241 ttggttttgg gcgcagccat attgcgccag ctattaccga actgatgcgc aattatcctg
    45301 agttacaggt gcattttgaa ttgttcgatc gacaaattga tttagtgcag gataatattg
    45361 atctggatat tcgtattaat gacgcaattc ctgattatta tattgcgcat cttttgacaa
    45421 aaaataaaag aatattatgc gcagcacctg agtatctgca aaaatatccg cagccacaat
    45481 ccttacagga attaagtcgt catgactgcc tggtgaccaa agagcgcgat atgacccacg
    45541 gaatatggga gttggggaac ggtcaggaga aaaaatcggt gaaggtaagc ggacatcttt
    45601 cctccaatag cggcgagatt gtgctgcaat gggcgctgga gggcaaaggg attatgctgc
    45661 gttccgagtg ggatgtgttg ccgtttctgg agagtggcaa actggtgcga gtattgccgg
    45721 agtatgcaca aagcgccaat atctgggctg tttaccggga gccgctctat cgcagcatga
    45781 aattacgtgt ctgtgtggaa tttctggcgg catggtgcca gcaacggctg ggcaagcccg
    45841 acgaaggcta tcaggtcatg tagatccacc agaaatcact cgggatcctt ttcgcgcttt
    45901 cttttgaatt tcgtccagat ttttgtctcc tgacgacgcc acagacgctg gatgttgtca
    45961 tgatgacgca gcaggatcag gcaagagagc atcgaaaccg ggaaggtgaa ttgtggctta
    46021 aaccaccaga cataaaacgg agcaatcagt gcactgacaa tcgctcccag cgacgagtaa
    46081 ccactcaata gcacggtcag taaccaggtt cccgccatta ctccagtgag atcccagcca
    46141 attggtgcga tggcaccaaa agcggtagca acgccttttc ctcctttaaa accgaagaaa
    46201 acgggccaga tgtgtccaag acaggcggcg atggcaatta agcctagcca gaaggggctg
    46261 acacctaatt catacgcgcc ccagacgggc aacattcctt tcagaacgtc gaaaatcagt
    46321 actgctacgg ctgctccctt gccaccgata cgtaacacat tggttgcgcc tggattgccg
    46381 gagccgctgg ttcgcggatc gggcaaccca cacaagcggc aaaccagaat ggcactggaa
    46441 atggagccgc agaggtacgc gatgaggatc attccaggcg cgattgcact cataagctgt
    46501 tccgttttga aaattcgtgt tcaacgatga atctgtggat aatacgcaca tttcgccgga
    46561 agtggtatcc ggttagccaa aaagcaggca ggacgtgatg gatattgtat ttatagagca
    46621 actttcggta atcaccacta ttggtgttta cgactgggaa cagaccatcg aacagaagtt
    46681 agtgttcgat atcgaaatgg cgtgggataa ccgtaaagcg gcgaaaagtg atgatgtggc
    46741 ggattgcctc agttacgctg acattgcaga aacggtggtc agccacgtcg agggggcgcg
    46801 ttttgcgctg gtggaacgcg tggctgaaga ggtggcggag ctgctgttag cacgcttcaa
    46861 ctcgccgtgg gtgcgtatca aactcagcaa gccaggcgca gtggcgcggg cggcgaatgt
    46921 tggcgtaatc attgagcgtg gcaataatct gaaagaaaat aattaatttt acagctgtta
    46981 aaccaaacga ttataacctg gtcatactgc agtagttcgg acaagcgtta catttttata
    47041 atttaggggt ttattgatga gcgatatgca ctcgctgctg atagcggcaa tattgggtgt
    47101 ggtcgaagga ttgacagaat ttctgccggt atccagcacg ggccatatga ttattgtcgg
    47161 tcacttgttg gggtttgagg gcgacacggc gaaaaccttt gaagtcgtga tccagttagg
    47221 atcaattctg gcggtagtag tgatgttctg gcggcgtctg tttggcctga ttggcatcca
    47281 ctttggtcgc ccgttgcagc acgaaggaga aagcaaaggt cgtttaacgc tgatccacat
    47341 cttgctgggg atgattccgg cggtggtact ggggctgttg ttccacgaca cgattaaatc
    47401 attgtttaac ccgataaatg tcatgtatgc actggtcgtt ggcggtctgt tgctgattgc
    47461 cgccgaatgc ctgaagccga aagagccgcg tgcgccgggt cttgatgata tgacctatcg
    47521 tcaggcgttt atgattggct gtttccagtg tctggcgctg tggccgggtt tctcccgttc
    47581 cggggcgacc atttcaggtg ggatgctgat gggggtgagc cgttacgctg cttccgagtt
    47641 ctcgttcctg ctggcggtgc cgatgatgat gggcgcaacg gcgctcgatc tctacaaaag
    47701 ctggggcttc ctgactaccg gcgatatccc gatgtttgcc gttgggttta tcaccgcttt
    47761 tgtggtggcg ctgatagcga ttaaaacctt cctgcaattg attaagcgca tttcgtttat
    47821 cccgttcgcc atttatcgct ttattgtggc ggctgcggtg tacgttgtgt tcttttgatg
    47881 tgaagaacag tgtcggatat gatgctgacg cgtcttatca gattgcgtag gccggataag
    47941 gcgttcacgc cgcatccggc aatcaacgcc tgatgcgacg ctcgcgcgtc ttatcaggcc
    48001 tacaactgtc gttgacatgt agccggataa gacgcttgcg tcgcatccgg caaaatatcc
    48061 aacactcact caggttttgg gcaacgctgt tccttccagc tggctaccgc cgcaatccgc
    48121 cgtcgggtca gctcctcacg aatctccaca cctttaaatc ctgcttcaac gacagctttt
    48181 gtcggcactg actgcgccac ttcccaggct tcgcgcaacc agcggccttg cgggtagtcc
    48241 gccgattcaa aaccggttct gccgcgcacg tcagcctcgc tggtcagcgc cagttgctcg
    48301 acacgctgcg gtttacgcca ggcatcaatg gaatcaaaca atttaacaat ggttttcggg
    48361 ttcagcattg gaaaggtgtg gatgagatcg tgaaactcag ctaccagtct ggctaaatcg
    48421 cgaatttcat ttggcacgcg cagacgctgg catagttgtt ccactaactt cacacccgcc
    48481 gggccatgac catgatgacg tggccaaagt tctggcggcg tcagtccttt accgagatcg
    48541 tggcataacg ttgcgaaacg gacatcgacc tgcggactca gcatcgccgc cattgagagc
    48601 gtcattaagg tatgaatacc cgtgtcgatt tccggatgcc acctggcagg ggccggaacg
    48661 ccaaacaggg cgtcaatttc cgggaataaa acgcgcagtg cgccgcaatc gcgcagtacc
    48721 tggaagaaca cctggggatt gcgggtggtg agggcgtttt ccgtctcttt ccatacccgt
    48781 tcaggcgtca ggtgttccag ttcacctgca tgggtcatct cgcgcatcag cgccagagtt
    48841 tcatcggcaa tacggaaact gaggtgggca taacgcgcag caaaacgcgc tacgcgcaac
    48901 acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc gcaacagacg attttgcaga
    48961 tcgcccagac cgttgtacgg gtcgataatc tcgccgttat cgtcctgggc cagcgcatta
    49021 atggtcagat cgcgacgctt aagatcatct tccagcgtga catccggtgc ggcatagcag
    49081 gtaaaaccgg tgtaaccgga accggatttc cgttcagtac gtgccagcgc atactcttca
    49141 tgcgtttgcg gatgcagaaa aacaggaaaa tcgcggccta cctgctggta gcccgcgtcg
    49201 agcatctcct gtggcgtact gccgaccacc acccaatctc tgtctttgac cggtagccct
    49261 aacaatgcat cccgaacagc accaccgacc agataaatct tcacgccatc atcccttcgt
    49321 caaatacaca attcctaata ataagacagt gcaacgaaga aggcgattta gttcatccag
    49381 cgatctttgc gtttgcggct tgggatcagg tgcggcagta ccagaccaag cagcaagcca
    49441 agccccagca cgccaccgcc atacataaac cactgcatga tgatggtgcg ctgtttgtca
    49501 tccagctgta cgctggcggc atcgaccttt ttctgcgcga caatcagctc gtttttcagt
    49561 ttctgatttt cttcttttaa cccgttgatc acgctgtcac tctgcgccac tttttgctgc
    49621 atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg tgagtttatc ggtcagcgtt
    49681 ttgacctgat tttccagatc tggcacacgg gagcgcaggc ttggctctgt gctaagttgt
    49741 ttcaacggga tccaggcggt acggccagag ctgtctttca cctgggcata attggtgttg
    49801 gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa ccgtgcccac gaggcgataa
    49861 tgatctcccg gaccgctacg gacccaggta ttcagttcat cagaaacata gcgcgtttct
    49921 tcagcatgtg agacggcagt ggcgctaagt gcgagtaaag ttaatccgat caggcgtaat
    49981 tttggcatca ggctgtcgtt attgtcatgg aaagtggaac gatagtagtg gcatcagtgt
    50041 cgctacgcaa agcattcgac atcaatcgga atcctctgtg tcaggtcttg ccactgcttc
    50101 aaacttttac gcccgtcaaa ttgcccgttg catggcaatt tggcgcaaaa tactatctac
    50161 tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac ttatgtgaca taaccgataa
    50221 gtaaaggcta tggctcagga aatcgaatta aagtttattg ttaatcacag tgccgttgcg
    50281 gcgctgcgtg accatctcaa tacgctgggc ggcgagcacc atgaccccgt gcagttgctg
    50341 aatatttact acgaaacgcc ggataactgg ctgcgtgggc acgatatggg cttgcgtatt
    50401 cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg caggaagagt gacaggcggc
    50461 ttacaccagc gcccggaata taacgtggcg ttgagcgaac cgacgctcga cctggcgcag
    50521 ttaccgacgg aagtctggcc gaacggcgaa ttgcctgccg atctcgcttc ccgcgtacaa
    50581 ccgctgttca gcaccgattt ttatcgcgaa aagtggctgg tagaggttga tggtagccaa
    50641 attgaaatcg ccctcgacca gggggaagtg aaagcgggtg aatttgctga acctatctgt
    50701 gagctggaac tggaactgct tagcggcgac atgcgtgcgg tgctgaaact ggcgaaccaa
    50761 ctggtatcgc aaaccggatt acgccagggc agcctgagca aagcagcgcg tggctatcat
    50821 ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca ccattttgca tgttgcggca
    50881 aaagctgatg tggaacaggg gctggaagtg gcgctcgagc tggcgttagc gcaatggcag
    50941 tatcatgaag aactgtgggt acgcggcaac gatgcggcga aagagcaggt gctggcagcc
    51001 attggcctgg tccgtcatac actgatgctg ttcggcggta tcgtgccgcg taaagcgagc
    51061 actcacttac gtgatctgct gactcaatgc gaggcgacca ttgcttctgc ggtttctgcc
    51121 gtaacggcgg tctactctac cgaaacggca atggcgaagc tggcgttgac cgaatggctg
    51181 gtaagcaaag cctggcagcc gtttttagat gccaaagcgc agagtaaaat gagcgattcc
    51241 ttcaaacgct ttgccgatat ccatctttcc cgccatgctg ctgaacttaa aagcgttttc
    51301 tgccagccgt taggcgatcg ctaccgtgac caattgccac gcctgacgcg tgatattgac
    51361 tcaatactgt tgctggcggg ttactacgat cctgtcgtcg cgcaagcctg gctggagaac
    51421 tggcaggggc tgcgtcacgc tattgcgacc gggcaacgca ttgaaattga acatttccgt
    51481 aatgaggcaa acaatcagga accgttctgg ttgcacagcg gaaaacgtta atcaggcaag
    51541 gataaaatcg cttatgaagc cgctctcttc accgttacag cagtactggc agaccgttgt
    51601 tgagcggctg ccagagcctt tagccgagga atcacttagc gcacaggcga agtcagtact
    51661 tactttcagt gattttgtgc aggacagcgt gattgcgcat ccagagtggc tgacggaact
    51721 ggaaagccaa ccgccgcagg ccgacgaatg gcagcattac gctgcatggt tgcaggaggc
    51781 gctcagtaat gtgagtgacg aagccgggtt aatgcgcgag ctgcggctat tccggcggcg
    51841 cattatggtg cgcatcgcct gggcgcaaac gctggctctg gttactgaag agagcatatt
    51901 gcagcagctc agccatctgg cggagacgct gattgttgcg gcgcgtgact ggctgtatga
    51961 cgcctgctgc cgcgaatggg gaacgccgtg caatgcgcag ggcgaagcgc aaccgctgct
    52021 gattttaggc atgggtaagc tgggcggcgg ggaactgaat ttctcctctg atatcgatct
    52081 gatttttgcc tggccggaac atggttgtac ccagggtgga cgccgggaac tggataacgc
    52141 gcagtttttt acccgcatgg ggcagcggct gattaaagtg ctggatcaac caacgcagga
    52201 tggcttcgtc tatcgcgtgg atatgcggct gcgtccgttt ggtgaaagtg gcccgctggt
    52261 gctgagtttt gcggcgctgg aagattatta ccaggagcag gggcgcgact gggagcgtta
    52321 cgcgatggtc aaggcgcgga ttatggggga tagcgacggc gtctatgcta acgagttgcg
    52381 tgcgatgctg cgtccgtttg ttttccgtcg ttacatcgat ttcagcgtga ttcagtcgct
    52441 gcgcaacatg aaaggaatga ttgcccgtga agtgcgtcga cgcggtttga ccgacaacat
    52501 caaacttggc gcaggcggca ttcgcgaaat tgaatttatc gtccaggtgt tccagctcat
    52561 tcgcggcggg cgcgaaccgt cgctgcaatc gcgcgcttta ctgccaacgc tcagcgccat
    52621 tgccgcgctg catctgcttt ctgaaaacga tgctgaacaa ttgcgagtgg cgtatctgtt
    52681 cctgcggcgt ctggaaaacc tgctgcaaag cattaacgac gaacaaaccc agacgcttcc
    52741 ttctgatgag cttacccgtg cgcggctggc gtgggcgatg gactttgctg actggccgca
    52801 attgaccggg gtgctgaccg cacatatgac caatgtgcgc cgggtgttta atgaattgat
    52861 tggcgacgac gaaagtgaaa ctcaggaaga gtcgctgtcg gaacagtggc gtgagctgtg
    52921 gcaggatgcg ttgcaggaag atgacaccac gccagtgctg gcgcatctta gcgaggatga
    52981 tcgcaaacag gtgctaatgc tgattgctga ttttcgcaaa gagctggata agcgcaccat
    53041 cgggccgcga ggacgtcagg tgctcgacca tctgatgcca catctgctaa gtgatgtctg
    53101 tgcgcgtgaa gacgctgctg tcacgctgtc gcgcattacc gccttgctgg tggggattgt
    53161 tactcgcacc acttatcttg agctgcttag tgaattccct gcggcgctta aacatttgat
    53221 ttctctgtgt gctgcatcgc cgatgattgc cagccagcta gcgcgttatc cattattgct
    53281 ggatgaattg ctcgatccaa ataccctcta ccaaccaacg gcgacggatg cctaccgtga
    53341 tgaactgcgc cagtatttgc tgcgcgtgcc ggaagatgac gaagaacaac agcttgaggc
    53401 gctgcgtcag ttcaaacagg cgcagctgtt acgcattgcg gcagcggata tcgccggtac
    53461 gctaccggta atgaaagtga gcgatcactt aacctggctg gcagaagcca tgattgatgc
    53521 cgtcgttcag caggcgtggg ttcaaatggt tgcccgctac ggtaagccaa atcacctgaa
    53581 cgaacgcgaa gggcgtggtt ttgcggtggt cggctacggc aagctggggg gctgggagtt
    53641 aggctacagt tccgatcttg acctgatctt cctccatgat tgcccaatgg atgcgatgac
    53701 tgacggcgag cgggaaatcg acgggcgaca gttttatctg cgtctggcgc aacgcattat
    53761 gcatctgttc agtacgcgaa cctcttccgg cattttgtat gaagtggatg cacgactgcg
    53821 tccgtccggg gcggcgggaa tgctggtgac atccgcagaa gcatttgctg attatcagaa
    53881 aaacgaggcc tggacgtggg agcatcaggc gctggtgcgt gcgcgtgtag tgtacggcga
    53941 tccgcagctc accgcgcact ttgacgcagt acgccgggag attatgacgc tgccgcgcga
    54001 aggcaaaacc ctgcaaacag aagtgcggga aatgcgcgag aaaatgcgtg cccatctcgg
    54061 caataaacat cgcgatcgct ttgatatcaa agccgatgaa ggcggaatta ccgatatcga
    54121 atttattact caatatctgg tgttgcgcta cgctcatgaa aaaccgaagt taacgcgctg
    54181 gtcagacaac gtgcgcattc tggaactact ggcgcaaaac gacattatgg aagagcagga
    54241 agcgatggcg ctgacccgtg cttacacaac gctgcgcgat gaacttcatc atctggcatt
    54301 acaggaattg ccgggccatg tgtcggagga ttgcttcacc gcagagcgtg aactggtgcg
    54361 ggcaagctgg cagaagtggc tggtggaaga atgaagtatg gtattatcgc gcgcaaattt
    54421 tgaatctctc aggagacagg aatgaaagta acgctgccag agtttgaacg tgcaggagtg
    54481 atggtggttg gtgatgtgat gctggatcgt tactggtatg gtcccaccag ccgtatctcg
    54541 ccggaagcgc cggtgcccgt ggttaaagtg aataccatcg aagaacgtcc gggcggcgcg
    54601 gctaacgtgg cgatgaatat cgcttctctc ggtgctaatg cacgcctggt cgggttgacg
    54661 ggcattgacg atgcggcgcg cgcgctcagt aaatctctgg ccgacgtcaa cgtcaaatgc
    54721 gactttgttt ctgtaccgac gcatccgacc atcaccaaat tacgggtact ttcccgcaac
    54781 caacagctga tccgtctgga tttcgaagaa ggtttcgaag gggttgatcc gcagccgctg
    54841 cacgagcgga ttaatcaggc actgagttcg attggcgcgc tggtgctttc tgattacgcc
    54901 aaaggtgcgc tggcaagcgt acagcagatg atccaactgg cgcgtaaagc gggtgttccg
    54961 gtgctgattg atccaaaagg taccgatttt gagcgctacc gcggcgctac gctgttaacg
    55021 ccgaatctct cggaatttga agctgttgtc ggtaaatgta agaccgaaga agagattgtt
    55081 gagcgcggca tgaaactgat tgccgattac gaactctcgg ctctgttagt gacccgttcc
    55141 gaacagggta tgtcgctgct gcaaccgggt aaagcgccgc tgcatatgcc aacccaggcg
    55201 caggaagtgt atgacgttac cggtgcgggc gacacggtga ttggcgtcct ggcggcaacg
    55261 ctggcagcgg gtaattcgct ggaagaagcc tgcttctttg ccaatgcggc ggctggtgtg
    55321 gtggtcggca aactggggac atccacggtt tcgccgatcg agctggaaaa cgcagtacgt
    55381 ggacgtgcag ataccggctt tggtgtgatg accgaagagg aactgaagct tgctgtagcg
    55441 gcagcgcgta aacgcggtga aaaagtggtg atgaccaacg gtgtctttga catcctgcac
    55501 gctgggcacg tctcttatct ggcaaatgcc cgtaaactgg gtgaccgttt gattgtcgct
    55561 gtgaacagcg atgcctccac caaacggctg aaaggggatt cccgccccgt taacccgctc
    55621 gaacagcgta tgattgtgct gggcgcactg gaagcggtcg actgggtggt gtcgtttgaa
    55681 gaggacacgc cgcagcgctt gatcgccggg atcttgccag atctgctggt gaaaggcggc
    55741 gactataaac cagaagagat tgccgggagt aaagaagtct gggccaacgg tggcgaagtg
    55801 ctggtgctca actttgaaga cggttgctcg acgaccaaca tcatcaagaa gatccaactg
    55861 gataaaaaag gctaactgga aagcggttca cagatctcgc ttcgtagcag gcaggtctat
    55921 tgacgctgtt gttttttcaa cctaaagtaa aggaacaagg gtaagggagg atttctcccc
    55981 cctctgatga gttgttagta agtcgggaaa cttaacagta acaacacaac cagtatgatg
    56041 acgaacttca tcataaccct ttccttctat aaggcccctt cctcgggagg ggctttcccg
    56101 tttcagcccc ttgctggctc cccgattgtt gatgagcgcc ggactccgcg caacgctttc
    56161 ccagaggcag ttttgccttc cccgagcagt cacgcaaaat gctgcaacag cctgatcatt
    56221 tgcacctttt agcggaacgc agatataaaa aatccccggc tttttgggcc agggattatt
    56281 catagaagag ggtttactct gctttttctt cgacgggagt tgttgagctt aaggatgaag
    56341 tcaatgccgc cagtgagccg cctgaaacgc caatttcatt gagcaaggag tcaatcagcg
    56401 gtgcctgtgt gcggtaagag agtgcggctg ataacgcttg ttctgccagg tttccgcccc
    56461 ccacattccc cgtgtttgca tcacccgtag cgccaccacg attcagccca tcgacctgaa
    56521 taattttgat gccgtcgata gccttcatag gctcaacgga tttctcaatt accgcaggca
    56581 gcgcctgcaa taatgccagt ttgaatttaa ggctggtttg ttcatcagaa agtacgttga
    56641 tagcatcgtt cagcgcacgt tgtgcttctg cttccgccag acccttttta cgtgtagctt
    56701 cggctaactc aacgatagcc gccgcctgca tctctgcggc ttctttttct gctttcgccc
    56761 gcacggtcag ttcaaccgct ttggtttctg catcctgcgc tgcggcgatc aacgccactt
    56821 gtttagcacg atcggcttcg gcagtctggc gagtggtttc tacgttttgt tgggcgctta
    56881 ctgcttctgc gagtgcaagg ttagcacgcg cttctgcctg ggactgttgt tccgatttgg
    56941 cggcaatagc gatcgatttc gtctggttag cgatttcggt gacctgctgc tgttcgatct
    57001 ctttaatgcg aacttcacgt tcggcctcaa cctttcttga gcggacggcc tgttcgcggt
    57061 cgatttccgt ttcctgaatc tgtcgttcag ccagaattcg cgtctgctct gcttcacgac
    57121 gacgttcagc ttcaaaagcc gcaattttcg cattctgctc agcggtacgg gttttaacct
    57181 gctgctcctg ctcaagcgtc ataaacgctt cttgttgttc aatctccaac ttgcgcgaaa
    57241 gtgcatcacg gtttttctca cgcactgcga cttccacatc ctgttcaact tcgttacgtt
    57301 cgcgacgacg gcgctccgtc tcctgagtca gtttggttaa accttcggcg tcaaaggcat
    57361 tgttcggatt gaaatgttct ttcgaggtct ggttaaagtt ggtaagtgaa acgctttcca
    57421 gttccaggcc atttttcgac agatcttcag ctacggtatt ttgtactccc tgcacgaagt
    57481 tctcgcgggt atcctgtaac tcatgcatgg tcatttgtgc cgctgttgca cggagggcat
    57541 cgacaaattt atcttccacc agcatacgta agtcttcagg cgacagggtg cgttgcccca
    57601 gcgtctgggc ggcggtggca atcccttcta ctgaaggttt tacccgcaca aagaaagcca
    57661 ctacaacatc aacacgcata cgatctttcg taatcaggct atcaatagta gaacggctga
    57721 cttccagctt cagggtattc atattgatgg gaattatttc atgaaagatc ggcatcacga
    57781 ttgcgccacc gctcatcacc actttttgcc cacttaaacc agtacgaaca aatgcttgct
    57841 ctgccgaagc gcgacgatag agcctggcga aaataattcc aataataaac agaatgcata
    57901 cggcgataat cgcggtaaac atccaggagg gcacagaatt aacgatatca tccattatta
    57961 aatttccttt tgattaataa atcacaatat ctggggccag ggattatttt ccgccaaata
    58021 tcgcgttgca gaaagtcggc aaataattaa cactttgtct cctttagtga aagtttttcc
    58081 ttcttcaggc tccagtagca aataatgtat ttggccaaat tgatcggtga gttttccttc
    58141 gcaagggtta cctgaagtgg cttgatggcc ggtaattaat gccatgctac caacatattc
    58201 ttcttctgta atagctgaac tatggtcgcg tggaatccag ggtgcaacga ctttaccggt
    58261 ataatgcact gcaataattg taaacagtaa actgacagga accacgaaca ggttggggag
    58321 tggtgattgc cagagcgtaa cgcaggcatg ttgcaacagg atgccaataa gaccaaaaaa
    58381 accagcgagt aagcagagga cgacgagagc tggtaatctt ccaatattga gataatgaag
    58441 tgcctggcta atatgacctg tggttatgga atcataatgg tcaagatgtg catcaagagc
    58501 accggagagc atgtgaccgc agataagtgc aaaaatttct aaaaggccga tcaagagtac
    58561 aaaggaaata gcgaaaagat aaggggtgtt atagtcggcg aataaaatca tatgtcactc
    58621 caatgtccat ttagtgacgc aatatttact ttggaatagt ttccttgctt acatttgtct
    58681 ttgttaataa taacacatgt tagagaggtt ctggaagaga aagtctttta atatggtttt
    58741 tttatttttc actaaaaagt gtgatcgggg acaatatatt tacgcacgtt atgtttaaag
    58801 gcactacact gattggggaa tactgaaatc agaaaaagca tatggcctta aaaggtatgc
    58861 aataaccgga ggatgtgctt atggatcata gtcttaattc tttaaataat ttcgattttc
    58921 tcgcgcgtag ttttgccaga atgcacgcag aaggtcgccc ggtcgatatt ctggccgtta
    58981 ctggtaacat ggatgaagaa catagaacct ggttttgcgc gcgttatgcc tggtattgcc
    59041 aacagatgat gcaaaccaga gaacttgaat tagaacactg atattacggg ccgtctggcc
    59101 cgtttttgtg gtgctttatt ctgctttatc aactggcggg atagccggag gagattgaag
    59161 ttctgctatc ggattattgc gcgcttccag ttcgctgatg cgctgctcca gtaacgccag
    59221 tttttcacga gtccgtaaca ggacttgcgt ctgaacgtcg aattcttcgc ggcttaccag
    59281 atcgagacgc gtcagctgcg cttgtagggt ttggcggatt tttttctcca catcttcccc
    59341 gaactccctg attcctttag gcattgattc gtgaacctgg cgagcgattt gctcaatttt
    59401 tttcgggtca atcattgtag tttccctgta ctgataggtg ttgagtgcca ttgtagtgcg
    59461 ataagggtaa gtcataaacc agaattatgt gaagctatgc gttgctggcg ctaatcatta
    59521 gcgttatagt gaatccgctt attctcaggg cggggcgaaa ttccccaccg gcggtaaatc
    59581 aactcagttg aaagcccgcg agcgctttgg gtgcgaactc aaaggacagc agatccggtg
    59641 taattccggg gccgacggtt agagtccgga tgggagagag taacgattct gtcgggcatg
    59701 gacccgctca cgttattttg gctatatgcc gccactccta agactgccct gattctggta
    59761 accataattt tagtgaggtt tttttaccat gaatcagacg ctactttcct cttttggtac
    59821 gcctttcgaa cgtgttgaaa atgcactggc tgcgctgcgt gaaggacgcg gtgtgatggt
    59881 gcttgatgat gaagaccgtg aaaacgaagg cgatatgatc ttcccggcag aaaccatgac
    59941 cgttgagcag atggcgctga ccattcgcca cgggagtggt attgtttgcc tgtgcattac
    60001 tgacgatcgc cgtaaacaac tcgatctgcc aatgatggta gaaaataaca ccagcgccta
    60061 tggcaccggt tttaccgtga ccattgaagc ggctgaaggt gtgactaccg gtgtttctgc
    60121 cgctgaccgt attacgaccg ttcgcgcagc gattgccgat ggcgcaaaac cttcagatct
    60181 gaatcgtccg ggccacgttt tcccacttcg cgcgcaggca ggtggtgtac tgacgcgtgg
    60241 cggtcatact gaagcgacta tcgatctgat gacgctggca ggctttaaac cagctggtgt
    60301 gctgtgtgag ctgactaatg acgatggcac gatggcgcgt gcaccagagt gcattgagtt
    60361 tgccaataaa cacaatatgg cgctcgtgac tattgaagac ctggtggcat accgtcaggc
    60421 acatgagcgt aaagccagct gaaaaccgct gcttaattta ctgccttaat caagaaaccg
    60481 aagttgtagc aggcttcggt ttttattttt ccctgctatg caagatgtta attccgcttc
    60541 tctttctatg aga
//
LOCUS       DAZFRE010000018        59360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFRE010000018 DAZFRE010000000
VERSION     DAZFRE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.018"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(105..1055)
                     /gene="dusC"
                     /locus_tag="ACHKUI_001344"
     CDS             complement(105..1055)
                     /gene="dusC"
                     /locus_tag="ACHKUI_001344"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6597582.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            1294..1692
                     /gene="yohJ"
                     /locus_tag="ACHKUI_001345"
     CDS             1294..1692
                     /gene="yohJ"
                     /locus_tag="ACHKUI_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6597583.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1689..2384
                     /gene="yohK"
                     /locus_tag="ACHKUI_001346"
     CDS             1689..2384
                     /gene="yohK"
                     /locus_tag="ACHKUI_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6597584.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHALLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2514..3398
                     /gene="cdd"
                     /locus_tag="ACHKUI_001347"
     CDS             2514..3398
                     /gene="cdd"
                     /locus_tag="ACHKUI_001347"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6597585.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            3548..4267
                     /gene="sanA"
                     /locus_tag="ACHKUI_001348"
     CDS             3548..4267
                     /gene="sanA"
                     /locus_tag="ACHKUI_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6597586.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4270..4509
                     /gene="yeiS"
                     /locus_tag="ACHKUI_001349"
     CDS             4270..4509
                     /gene="yeiS"
                     /locus_tag="ACHKUI_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6597587.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4703..5941
                     /gene="preT"
                     /locus_tag="ACHKUI_001350"
     CDS             4703..5941
                     /gene="preT"
                     /locus_tag="ACHKUI_001350"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6597588.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            5935..7170
                     /gene="preA"
                     /locus_tag="ACHKUI_001351"
     CDS             5935..7170
                     /gene="preA"
                     /locus_tag="ACHKUI_001351"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6597589.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(7413..8423)
                     /gene="mglC"
                     /locus_tag="ACHKUI_001352"
     CDS             complement(7413..8423)
                     /gene="mglC"
                     /locus_tag="ACHKUI_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6597590.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8439..9959)
                     /gene="mglA"
                     /locus_tag="ACHKUI_001353"
     CDS             complement(8439..9959)
                     /gene="mglA"
                     /locus_tag="ACHKUI_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6597591.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(10020..11018)
                     /gene="mglB"
                     /locus_tag="ACHKUI_001354"
     CDS             complement(10020..11018)
                     /gene="mglB"
                     /locus_tag="ACHKUI_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6597592.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11298..12338)
                     /gene="galS"
                     /locus_tag="ACHKUI_001355"
     CDS             complement(11298..12338)
                     /gene="galS"
                     /locus_tag="ACHKUI_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6597593.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(12480..13637)
                     /gene="yeiB"
                     /locus_tag="ACHKUI_001356"
     CDS             complement(12480..13637)
                     /gene="yeiB"
                     /locus_tag="ACHKUI_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6597594.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKHWKLHSGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSFRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(13654..14322)
                     /gene="folE"
                     /locus_tag="ACHKUI_001357"
     CDS             complement(13654..14322)
                     /gene="folE"
                     /locus_tag="ACHKUI_001357"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6597595.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14580..15416
                     /gene="yeiG"
                     /locus_tag="ACHKUI_001358"
     CDS             14580..15416
                     /gene="yeiG"
                     /locus_tag="ACHKUI_001358"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6597596.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15448..17439)
                     /gene="cirA"
                     /locus_tag="ACHKUI_001359"
     CDS             complement(15448..17439)
                     /gene="cirA"
                     /locus_tag="ACHKUI_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO6597597.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17731..19200)
                     /gene="lysP"
                     /locus_tag="ACHKUI_001360"
     CDS             complement(17731..19200)
                     /gene="lysP"
                     /locus_tag="ACHKUI_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6597598.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19405..20286)
                     /gene="yieE"
                     /locus_tag="ACHKUI_001361"
     CDS             complement(19405..20286)
                     /gene="yieE"
                     /locus_tag="ACHKUI_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6597599.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20385..21434
                     /gene="yeiH"
                     /locus_tag="ACHKUI_001362"
     CDS             20385..21434
                     /gene="yeiH"
                     /locus_tag="ACHKUI_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6597600.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21508..22365
                     /gene="nfo"
                     /locus_tag="ACHKUI_001363"
     CDS             21508..22365
                     /gene="nfo"
                     /locus_tag="ACHKUI_001363"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6597601.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFAGIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22369..23457
                     /locus_tag="ACHKUI_001364"
     CDS             22369..23457
                     /locus_tag="ACHKUI_001364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6597602.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPTHTTVDSFGADDGFMAGLVYSFLEGYSFRDSAHFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23725..24666)
                     /gene="rihB"
                     /locus_tag="ACHKUI_001365"
     CDS             complement(23725..24666)
                     /gene="rihB"
                     /locus_tag="ACHKUI_001365"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6597603.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            24796..25494
                     /gene="yeiL"
                     /locus_tag="ACHKUI_001366"
     CDS             24796..25494
                     /gene="yeiL"
                     /locus_tag="ACHKUI_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6597604.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(25561..26811)
                     /gene="psuT"
                     /locus_tag="ACHKUI_001367"
     CDS             complement(25561..26811)
                     /gene="psuT"
                     /locus_tag="ACHKUI_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6597605.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(26905..27843)
                     /gene="psuG"
                     /locus_tag="ACHKUI_001368"
     CDS             complement(26905..27843)
                     /gene="psuG"
                     /locus_tag="ACHKUI_001368"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6597606.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVIALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(27831..28772)
                     /gene="psuK"
                     /locus_tag="ACHKUI_001369"
     CDS             complement(27831..28772)
                     /gene="psuK"
                     /locus_tag="ACHKUI_001369"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO6597607.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(29228..30919)
                     /gene="fruA"
                     /locus_tag="ACHKUI_001370"
     CDS             complement(29228..30919)
                     /gene="fruA"
                     /locus_tag="ACHKUI_001370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6597608.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(30936..31874)
                     /gene="fruK"
                     /locus_tag="ACHKUI_001371"
     CDS             complement(30936..31874)
                     /gene="fruK"
                     /locus_tag="ACHKUI_001371"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6597609.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(31874..33004)
                     /gene="fruB"
                     /locus_tag="ACHKUI_001372"
     CDS             complement(31874..33004)
                     /gene="fruB"
                     /locus_tag="ACHKUI_001372"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6597610.1"
                     /translation="MFQLSIQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDALTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            33372..34553
                     /gene="setB"
                     /locus_tag="ACHKUI_001373"
     CDS             33372..34553
                     /gene="setB"
                     /locus_tag="ACHKUI_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6597611.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLTCTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAELEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(34550..34804)
                     /gene="yeiW"
                     /locus_tag="ACHKUI_001374"
     CDS             complement(34550..34804)
                     /gene="yeiW"
                     /locus_tag="ACHKUI_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6597612.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            34959..35531
                     /gene="yeiP"
                     /locus_tag="ACHKUI_001375"
     CDS             34959..35531
                     /gene="yeiP"
                     /locus_tag="ACHKUI_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6597613.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            35754..37220
                     /gene="yeiQ"
                     /locus_tag="ACHKUI_001376"
     CDS             35754..37220
                     /gene="yeiQ"
                     /locus_tag="ACHKUI_001376"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6597614.1"
                     /translation="MNTIASITLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            37338..38324
                     /gene="yeiR"
                     /locus_tag="ACHKUI_001377"
     CDS             37338..38324
                     /gene="yeiR"
                     /locus_tag="ACHKUI_001377"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6597615.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            38363..39076
                     /gene="lpxT"
                     /locus_tag="ACHKUI_001378"
     CDS             38363..39076
                     /gene="lpxT"
                     /locus_tag="ACHKUI_001378"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO6597616.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            39488..40054
                     /gene="mepS"
                     /locus_tag="ACHKUI_001379"
     CDS             39488..40054
                     /gene="mepS"
                     /locus_tag="ACHKUI_001379"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6597617.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            40235..41791
                     /gene="pdeN"
                     /locus_tag="ACHKUI_001380"
     CDS             40235..41791
                     /gene="pdeN"
                     /locus_tag="ACHKUI_001380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6597618.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            41873..43687
                     /gene="yejA"
                     /locus_tag="ACHKUI_001381"
     CDS             41873..43687
                     /gene="yejA"
                     /locus_tag="ACHKUI_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6597619.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            43688..44782
                     /gene="yejB"
                     /locus_tag="ACHKUI_001382"
     CDS             43688..44782
                     /gene="yejB"
                     /locus_tag="ACHKUI_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6597620.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            44782..45807
                     /gene="yejE"
                     /locus_tag="ACHKUI_001383"
     CDS             44782..45807
                     /gene="yejE"
                     /locus_tag="ACHKUI_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6597621.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFSWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            45809..47398
                     /gene="yejF"
                     /locus_tag="ACHKUI_001384"
     CDS             45809..47398
                     /gene="yejF"
                     /locus_tag="ACHKUI_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6597622.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(47402..47746)
                     /gene="yejG"
                     /locus_tag="ACHKUI_001385"
     CDS             complement(47402..47746)
                     /gene="yejG"
                     /locus_tag="ACHKUI_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6597623.1"
                     /translation="MTPLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(48079..49269)
                     /gene="bcr"
                     /locus_tag="ACHKUI_001386"
     CDS             complement(48079..49269)
                     /gene="bcr"
                     /locus_tag="ACHKUI_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6597624.1"
                     /translation="MTTRQHSSFAIVFILGLLTMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(49297..49992)
                     /gene="rsuA"
                     /locus_tag="ACHKUI_001387"
     CDS             complement(49297..49992)
                     /gene="rsuA"
                     /locus_tag="ACHKUI_001387"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6597625.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            50142..51902
                     /gene="radD"
                     /locus_tag="ACHKUI_001388"
     CDS             50142..51902
                     /gene="radD"
                     /locus_tag="ACHKUI_001388"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6597626.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHEKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52027..52311
                     /gene="rplY"
                     /locus_tag="ACHKUI_001389"
     CDS             52027..52311
                     /gene="rplY"
                     /locus_tag="ACHKUI_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6597627.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(52450..53457)
                     /gene="yejK"
                     /locus_tag="ACHKUI_001390"
     CDS             complement(52450..53457)
                     /gene="yejK"
                     /locus_tag="ACHKUI_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6597628.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            53639..53866
                     /gene="yejL"
                     /locus_tag="ACHKUI_001391"
     CDS             53639..53866
                     /gene="yejL"
                     /locus_tag="ACHKUI_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6597629.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            53886..55646
                     /gene="yejM"
                     /locus_tag="ACHKUI_001392"
     CDS             53886..55646
                     /gene="yejM"
                     /locus_tag="ACHKUI_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6597630.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAVGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            55721..55797
                     /locus_tag="ACHKUI_001393"
     tRNA            55721..55797
                     /locus_tag="ACHKUI_001393"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55755..55757,aa:Pro,seq:ggg)
     gene            complement(55923..56696)
                     /locus_tag="ACHKUI_001394"
                     /pseudo
     CDS             complement(55923..56696)
                     /locus_tag="ACHKUI_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            57016..57663
                     /gene="narP"
                     /locus_tag="ACHKUI_001395"
     CDS             57016..57663
                     /gene="narP"
                     /locus_tag="ACHKUI_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6597631.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(57698..58750)
                     /gene="ccmH"
                     /locus_tag="ACHKUI_001396"
     CDS             complement(57698..58750)
                     /gene="ccmH"
                     /locus_tag="ACHKUI_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6597632.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(58747..59304)
                     /gene="dsbE"
                     /locus_tag="ACHKUI_001397"
     CDS             complement(58747..59304)
                     /gene="dsbE"
                     /locus_tag="ACHKUI_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6597633.1"
                     /translation="MKRKVLLIPLIIFLAIATALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        14619 a        15423 c        14523 g        14795 t
ORIGIN      
        1 ccttgcgcca gtggcaatat gtggcttagc tcatgaaagg agcgcaacaa gatgatgaag
       61 ctgaataagc gattcattgt ctcagataag ctgcgattcc actattatcg cagcttatcg
      121 atatcaatgg cctgaatagc ccttgcaata tcaggggaat tattcaacac ccgaacatgc
      181 tgaaataatt ccgttgcttc atcgtattct ttacgcaaat aactcaacca ctgtttaatc
      241 cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca gacgggtata tttttgcagc
      301 aaagcaacca cctccggcca cggcattcgc ggttcgttat atttcaccac acggctcagg
      361 ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt cgcagccgct gatcgccatg
      421 cattgttgcg cactctgcca gtcccagatt tcaccgttgg caatcaccgg aatattaagc
      481 cgctggcgaa tctcgccaat cgcctgccag tcaatatgtt ccgcgcggta accctgctct
      541 tttgtccgcc catgtactac cagctccgta gcaccagcct gttgaaccgc atcggcgatt
      601 tcaaatttct tctcaccgct gtcccagccc agacgcactt ttacgctgac aggcaaatgc
      661 gccggtacag cttcgcgcat cgcttttgca ccctggtaga tgagttcagg atctttcagt
      721 aacgttgccc caccaccgct accgttaacc gttttcgacg ggcagccgca gttaagatcc
      781 acgccccagg aacctaactc caccgcacgg gcggcgttct ctgccagcca ctgcgggaac
      841 tgccctaaca actgcacgcg aaccagcgta ccggatggtg tccggctggc attttgtagc
      901 tcagggcaaa tgcgatgaaa gacttttact ggcagcagtt gatccaccac gcggacaaac
      961 tcggtgatgc acagatcgta gtcgttaact tcggtcagta attcccgcac cagagagtca
     1021 agtacaccct ccatcggagc cagtaacaca cgcatatcat cacccgcaaa aaaatgaggc
     1081 gctatgttag cgcctccggt caacggatta aaggtctgca aagagggatt gctgcaaact
     1141 gcaatccctc tctatgcttg atgtatgcct gagttcgctg cgcattgcgt aataccgggg
     1201 agcgaaaaaa taagcgctga tttcttaatg tgatcggtag cacgttttac gaattaattg
     1261 tatgatgaat ccatctcatc tggggtgttg attatgagta agacactgaa cattatctgg
     1321 caatatttac gtgcttttgt cctgatttat gcctgcctgt atgcaggcat tttcattgct
     1381 tccctgctac cggtaaccat tccgggcagc atcatcggga tgctgatcct gtttgtcctg
     1441 ctggccttgc aaattcttcc ggcgaaatgg gtcaatccgg ggtgctacgt actgattcgc
     1501 tatatggcgc tattgtttgt gccgattggc gtaggcgtca tgcaatattt tgatttgctc
     1561 cgcgcacagt ttggtccggt agtggtttcc tgtgcagtca gtacgctggt ggtttttctg
     1621 gtggtgagct ggagttcaca actggtgcac ggtgaacgta aagtcgtagg tcagaaagga
     1681 tcagaagaat gatggcgaat atctggtggt cattaccgct gacgttgatt gtcttttttg
     1741 ccgcccgcaa actggcggca cggtataagt ttcccctgct taacccgttg ctggtagcaa
     1801 tggtggtgat cattcctttt ttaatgctga ctggcatctc ttacgacagc tactttaaag
     1861 gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt cgcactggcc tatcctttat
     1921 atgagcagct acaccagatc cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg
     1981 gcagcgtggt tgcgatggtg acggggactt ctgtggcatt gttgatgggc gcttcaccgg
     2041 aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc tattgcaatg gcagttggcg
     2101 gcagtattgg cggtattccg gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg
     2161 gcgctgtgtt tggccatgca ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg
     2221 gtctggcaat gggcacagcc tcgcacgccc tcggtacggc gcgctgcgcc gagctggatt
     2281 atcaggaagg tgcatttagt tcgctggcgc tggtgttatg cgggattatt acttcgctga
     2341 tcgcaccgtt ccttttcccg attattctgg cagtaatggg ctaaaatttg cgatgcgtcg
     2401 cgcatttttg atgtaagttt cacgcgttgc ataattaatg agattcagat cacatataaa
     2461 gccataacgg gttcgtaaac tgttatccca ttacatgatt atgaggcaac gccatgcatc
     2521 cacgttttca aaccgctttt gcccaacttg cggataactt gcaatctgca ctggaaccta
     2581 ttctggcaga caagtacttc cccgctttgt tgaccgggga gcaagtctca tcgctgaaga
     2641 gcgcaacggg gctggacgaa gacgcgctgg cattcgcact acttccgctg gcggcagcct
     2701 gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa
     2761 cctggtattt cggtgccaat atggaattta ttggtgcgac aatgcagcaa accgttcatg
     2821 ccgaacaaag cgcgatcagc cacgcctggc tgagtggtga aaaagcgctt gcagccatca
     2881 ccgttaacta cacgccttgt ggtcactgcc gtcagtttat gaatgaactg aacagcggtc
     2941 tggatctgcg tattcatctt ccgggacgcg aggcacacgc gctgcgtgac tatctaccgg
     3001 atgcctttgg gccgaaagat ctggagatta aaacgctgct gatggacgag caggatcacg
     3061 gctatgcgct gactggtgat gcgctttctc aggcggcgat tgcggcggca aaccgttcgc
     3121 acatgcctta cagtaagtcg ccaagcggtg tcgcgctgga atgtaaagac ggtcgtattt
     3181 tcagcggcag ctacgctgaa aacgccgcat tcaacccgac tctgccgccg ttgcaggggg
     3241 cgttaattct gttgaatctc aagggttatg attacccgga tatccagcgc gcggttctgg
     3301 cagaaaaagc cgatgcgccg ttgattcagt gggatgccac ctccgcaacg ctgaaagctc
     3361 tcggctgtca caatatcgac cgcgtgcttc tcgcttaagc ctggtgccgg atgcggcgtg
     3421 aacgccttgt ccggcttgcc agccctttcc tggtgtcgaa aatcccggca aacagtttgc
     3481 cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg aaattttcct tagatcgagt
     3541 ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc ctgatcggct tgctgctgtt
     3601 gactgtgctc ggcctcgatc gctggatgag ctggaaaacc gcgccttata tctacgacga
     3661 attgcaggat ctcccctacc gccaggtcgg tgtggtgctc ggcacagcaa aatattatcg
     3721 gactggcgta attaatcagt attatcgcta ccgcattcaa ggagcgatta atgcctataa
     3781 cagcggtaag gtaaattatc tattactgag cggcgataac gcattgcaaa gttataatga
     3841 gccgatgacc atgcgcaaag atttaatcgc agctggtgtc gacccatcag atattgttct
     3901 cgattacgca ggctttcgta cgctggattc catcgtgcgt acacgcaaag ttttcgatac
     3961 taatgatttc attattatca cccaacgttt ccactgtgag cgggcattat ttattgcgct
     4021 gcatatgggg attcaggctc agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt
     4081 acgtattcgc gaatttgccg cccgtttcgg tgcgctggct gacctttata tttttaaacg
     4141 tgaaccgcgt tttttagggc cgctggtccc tattccggct atgcaccagg tgccggaaga
     4201 tgcgcagggg tatcccgccg tcacacccga acagttactt gaattacaaa agaaacaagg
     4261 aaagtagtta tggatgtaca gcagtttttt gtcgttgccg tttttttcct tattccgata
     4321 ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa
     4381 aatgcaccag aaccggtgta tgtctggcga gcgaaaaatc ccggactctt cttcgcttat
     4441 atggtggcat atatcggctt cggaatttta tctatcggca tgattgttta tcttattttc
     4501 tatcgttaat attttccctt ttctgttaat tattgagaat aattattact tcactcaata
     4561 agctgcggat ataacattct gaaccgcagc tatttgtgaa tcctttcaca gtttaaattt
     4621 ccccacgcgc atagccttaa tatcagcacg caatatttat ttagcgttcg ccttaattac
     4681 tcatagcatt aaggaagatc acatgccgca acaaaattat ctggatgaac tcactccggc
     4741 ttttacgcct ttactggcga ttaaagaagc ctctcgctgt ttattatgtc atgacgctcc
     4801 ctgtagtcag gcttgcccgg cacagaccga tccggggaaa tttattcgct caatctactt
     4861 tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat aatgccctcg gtgccgtttg
     4921 tgccagagtg tgcccgacgg agaaattatg ccaaagcggt tgtacacgtg ccggtgttga
     4981 tgctcccatt gatatcggtc gtttacagcg ttttgttact gattttgaac aacaaactgg
     5041 gatggaaatt tatcagcccg gtactaaaac gctcggcaaa gtcgccatta ttggcgcagg
     5101 tcctgccgga ttgcaggcca gtgtgacgct gacaaaccag ggttatgacg tcacgatcta
     5161 tgagaaagaa gcacaacccg gtggctggtt gcgtaacggc attccgcaat tccggttacc
     5221 gcaatcggtg ctggatgcag aaatcgcccg tatagaaaaa atgggcgtga ccattaagtg
     5281 caataacgaa atcggtaaaa cacttaccct ggagcagctg aaagcggaaa accgcgcggt
     5341 actggtcacc gtagggttat cgagcggttc cgggttatcg ctgtttgagc atagcgacgt
     5401 tgagattgcc gtcgacttcc tgcaacgtgc acgacaggcg caaggcgata tcagcattcc
     5461 acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg gacgtagcca gcacgctgaa
     5521 agttctcggc tgtcaggcgg taacctgcgt agcgcgtgaa gagttagatg agtttccggc
     5581 aagcgaaaaa gagtttacca gcgccagaga gctgggcgtt tcgatcatcg atggatttac
     5641 accagtagcc gtggaaggca ataaagtcac ctttaagcat gttcgtttac cgggtgaact
     5701 aacgatggcg gcagataaaa ttattctcgc cgtcggtcag catgccagac tggatgcctt
     5761 tgcggagtta gagccgcagc gtaacaccat cgaaacgcaa cattaccaga cccgcgaccc
     5821 gcaagtcttt gctgctggcg atattgttga gggtgacaaa accgtggtct atgcagtgaa
     5881 aaccgggaaa gaagccgccg gggcgattca tcactattta gagggagctt gctcatgtta
     5941 acgaaagatc tttcgattac tttttgcggc gtgaagtttc ccaacccgtt ctgcctttct
     6001 tcttcgccgg taggcaactg ctatgagatg tgtgccaaag cctacgacac aggttggggc
     6061 ggcgtggtgt ttaaaacgat cggttttttt atcgccaacg aagtctcgcc gcgttttgat
     6121 catctggtga aagaagatac cggttttatc ggcttcaaaa atatggagca gattgctgaa
     6181 catccgttgg aagagaatct ggccgccctg cgtcggctga aagaagatta tccggacaaa
     6241 gtattgatcg cttcaatcat gggcgaaaat gagcagcaat gggaagagct ggcgcgtctg
     6301 gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct cctgtccgca aatgacttct
     6361 catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg tagaaaaata ttgtcgggca
     6421 gtaaaacgtg gttccacgct gccaatgctg gcgaagatga cgccgaatat tggtgatatg
     6481 tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg gcattgcggc gattaacacc
     6541 gttaaatcca tcaccaatat cgatcttaat cagaaaattg gtatgccgat cgttaacggt
     6601 aaatcgagta tttccggata ttccggtaaa gcggtaaaac cgatcgccct acgctttatt
     6661 cagcaaatgc gcacccatcc agaactgcgc gatttcccga tcagcggtat cggcggcatt
     6721 gaaacctggg aagatgcggc ggagttttta ttgctcggtg cagcgacgtt gcaggtgacc
     6781 accggcatta tgcagtacgg gtatcggata gtggaagata tggcaagcgg gttgtcgcat
     6841 tatctcgccg atcaagggtt tgattcgttg caggagatgg taggtctggc gaatcacaat
     6901 attgtcccgg cggaagattt agaccgcagt tatattgtct atccccatat caatcttgat
     6961 aaatgtgttg gctgtggacg ctgttatatt tcctgttacg acggcggtca ccaggcgatg
     7021 gaatggagcg agaaaacccg cacaccgcat tgtaataccg agaaatgcgt gggttgtctg
     7081 ctttgtggtc atgtctgccc ggtaggttgt attgatcttg gggaagtgaa gtttaagaaa
     7141 ggcgagaaag aacacccggt aacgttgtaa aaccagcgac gcatccggca tttagttcct
     7201 gtcgccggat gcggcgtgaa cgccttaccc ggcatacaaa acaatataaa tcagtatgtt
     7261 gtgctcattg taggccggat aaaacgcgcc agcgttgcat ccggcatttg gttcctgtcg
     7321 ccggatgcgg cgtaaacgcc ttatccggca tacaaaacca tacaactcat gatgttatgt
     7381 gcatttctga ccggatgcga actacattca catcacttct tacgcgcgta tttcagtgaa
     7441 tccagcgcca cggcgaagat aataatcgcc cctttgatga tgtactgcca gtacgggttt
     7501 acgccgatat acgtcaggcc atagttaatg acggtaaaaa taattacccc ggtcaccaca
     7561 ccaattaccg tccccacacc gccgctgaac gatacgccgc ctaccacgca cgccgcgata
     7621 gcatccagct catacataaa gccgaggttg ttggtggctg agccgatacg tccggcttct
     7681 aacatcccgc cgaaggcata gaacacacca gacaacgcgt aaatcatcag caggttcagg
     7741 ccgacgttga caccagatac tttcgccgct tccgggttac cgccaatggc aaaaatgttc
     7801 ttaccgaagc gggttttgtt ccataacacc cagacaaaca ccaccgcaat caacgcatag
     7861 aaggtgatgt aagagagacg gaaactcccc agcgcaataa agccttgggc aaaagtggag
     7921 aaaccactgt caaaaccgga aattggcgac gccccgacaa agtcgtaata gagcgagtta
     7981 atgccgtaga cgataatcat cgtaccgagc gtggtaatga acggcgtcac gttgagataa
     8041 gcgataatca agccgttgat caaaccgatc accgcaccaa tggcacagac aatcagaata
     8101 accagcgcaa tcggcatcgt cgccatttcc gggaacactt tgttggcgtt atccatggac
     8161 tgcaataacg tcgctgctac tactgccgcc agccccacct ggcgacctgc ggaaaggtca
     8221 gtcccttggg tgacaattaa ccctgccaca ccaagcgcga taataatacg caccgatgac
     8281 tgggtgagaa tattacttaa gttcaacaga cttaaaaatg ttgggtcctg gaaaataata
     8341 atcgccagca aaactaaaag aacgacgtaa ataccgccct ctttcaggta agtaagaaaa
     8401 cttttcttat ttaacgcact catgggaagc ccctaatctt aaaggtgcaa agacgcaaga
     8461 cgcagaattt cgctttgcgt tgttgttttt gtatcgacaa taccggaaac gagaccattg
     8521 ctcatgacca gaatacggtc tgttatccct aacaactctg gcatttcgga ggagataata
     8581 ataatcccct tgcctttctt cgccagttcg gcaattaact gataaatctc aaacttagcc
     8641 ccaacatcaa taccgcgcgt cggttcatcg agcattaata tttctggttg cgttaacagc
     8701 cagcgaccaa taatcacttt ttgctgatta ccaccggaga gcgaacctat ttgcgtccga
     8761 tgacctggcg tttttacccg catcgaatca atgacccact gggtatcgct tttcatccgc
     8821 gagttatcca gtaaaccaac tttattttta tagttgcgga tattggaaat taacgagtta
     8881 aagccaatat ccagataggc ataaattccc gttgagcggc gctcttcagt taccagtgca
     8941 aatccgtggt ttatggcttc gttggcatta tggttattga tctttttgcc gtgcaacgta
     9001 atggtgccag ccgatttctc gcgaatacca aataacgtct caacaatatc ggtacgtttc
     9061 gctcccacca gaccggctat accgagaatc tcccctttat gcagatcaaa cgagacatcg
     9121 cgaatcgacg gctggcgcag cgacgtcagg ttacgcactt ccagaatcac ttcgcccggc
     9181 ttgttttctt tgtcagggaa acgctggtta agagaacgcc caaccatcat ggcgatgatc
     9241 ttgtccatcg tcagccctgc cagcggttcg gtagcgatcc actgaccgtc gcgcaatacg
     9301 gtaacttcat cacataactg gaagatttct tccattttat gcgagatata aacaataccg
     9361 cagccgcgct cttttaattt acgaataata gtgaacagat gattgacctc tttttcggtt
     9421 aacgaagaag tcggttcatc cataatcaca attttcgcgt tataggaaaa cgctttggca
     9481 atttcgatca tctgcatttg cgaaacggat aatgtgccga cgcgtgcacg cggatcgata
     9541 tcaatatcca gttcatcaaa aatcgctttg gtttcgcggt acattttgtc ctgatcgaca
     9601 aacatgcctt tggtgggata tcgccccagc cacatgttgt ccatcaccga acgttgtaat
     9661 accaggttta actcctggtg taccatcgaa ataccatttt ccagtgcttc tttggcggaa
     9721 tggaaatcga tctctttacc ctggaataaa atggtgccgg agtctttttg ataaatacca
     9781 aacaggcatt ttaataatgt cgattttcct gcaccgtttt cccccattaa tgcatggata
     9841 gaatgtggtc ggacttttaa attaacgtta tcaagtgcct taacaccagg aaaagacttg
     9901 ttgataccgc tcatttccaa caagtattcc ccggaagacg gagtcgttga gctgaccata
     9961 taattttacc ttgttggcca tacaataagg gcgcagtaaa cgactgcgcc caatcagtct
    10021 tatttcttgc tgaattcagc caggttgtct ttatctacgc caacataagg tacgcggacc
    10081 actttgttgt cgattttcca gttggtgcca tcagccgcac ctttgccatc ggccaggttt
    10141 ttcgccagat caaaggtcgc tttcgcctgg ttgttagcat cgttcagtac ggtgcccgcc
    10201 agtgcaccgg atttcaccag cgccagcgct tctggcagcg catcgacgcc aaacaccgga
    10261 atgctggact tgttgtgtgc tttcagtgct tctaccgcgc ccattgccat cgcgtcgttg
    10321 ttggcgataa ccacctcgat tttgttggcg ttcgggccag acagccaggc gtccatctta
    10381 tctttcgcct gagcggtatc ccacatagcg gtatctaact gtaactgttc agttttaatg
    10441 cctttgtcgt tcaattcttt aatcacgtaa gtggtacgtg cttctgcatc cggatgcccc
    10501 ggctcacctt tcagcagtac gaattgaatc tgaccgtctt tgttcagatc ccaaccctga
    10561 ttcgccgccc agtgtttagc aatcaaatcg ccctgaataa tgccggactc tttggagtca
    10621 gtgccaacgt agtaggcttt gtcgtagcta tccagcgcct tacgagacgg ttctttgttg
    10681 aagaaaacca ccggcacgtt ttgcccacgc gctttctcaa tcaccgtacc cgcagctgcc
    10741 gggtcaacca ggttgattgc cagtgccttc acccctttcg ccagcaatac gtcgatctga
    10801 tcgttctgct tggactggtc attctgagaa tcattcatca gcagctgaac atctggcgcg
    10861 gctttcgcat cttgctcaat agccttgcgc actacagaca taaagttatc gtcgtactta
    10921 tagattgtta caccaatgcg agtatcagca gcgtgtgcag cggcaccgaa taacatgctg
    10981 gccatcacag cagacagggt taacaccttc ttattcatgg tatctccggt ttttcttatg
    11041 cagggtagtg cttgagataa atgctcggcg gggcagtaga gttaatgaag tgttactgaa
    11101 cgccgaagct cactttttaa aattcgttct tccatgcccg gtaacgctcc agaaaacggc
    11161 tttaattgtt gttatgacgc tattacctcg gtgaaagtga ttattcactg ttacatactg
    11221 gttacaacgt taaaacggtg caatcatagc catcacattg ttaagatact gtgaaatcac
    11281 tcacagattg aaagcggtta catcgcctga tttgttgagt tagtgatcgc cgccgcattc
    11341 tggcgtgttg cgacagaatg gcgacgcact aacgtcggca taaaacagtg gctggcacga
    11401 ggatcaatat tgtctgctgc cccctgcaag gccagttcgg tagctaattt agccattgaa
    11461 gcaatgggat aacgcaccgt cgttaattgc gggtcggtgt aacgggcgat gggaatatca
    11521 tcgaaaccaa tgattgagag atgtaacgga atcgcaatgc cattatcttt taatgctgtc
    11581 agtgcgccag cggccatatt gtcgttataa gcaaatacag cggttagttg taggttgcgc
    11641 cccagcagtt caaccatcgc cgcctcaccg cccggcatgt ccggcgtgcc agtgccaatc
    11701 cagctttccg gcggaataat atcctgctct ttcaacgcac tcatccagcc tgctttacgc
    11761 atggcgtcat cttcaatgcc gtggctggaa gaaagataac caatacgttg atgaccgtta
    11821 ttcagcaaca tgcgcgtcgc cattcgggca ccgctgagat tatccaggca aacgcaacga
    11881 tgggcgtacc ccggcacaac gcggttgatt aacaccatac cgggaatgtt atccataaat
    11941 tgtgccagtt catcgtcact caatgctttt gagtgaacaa tcaacgcatt acaacgctgg
    12001 cgaattaaca cctcaatggc gtgacgctct ttttccgctt catgatagct attgccgatt
    12061 agcacgtatt tctgatgctg ctgggcgacc agatccaccg cttttaccag cgcgccgaaa
    12121 aacgcatcag aaacgtccat caccaccacg ccaatggtat cgctaacctg agttgccagc
    12181 gcctgggcat tggcgtttgg ccgataatcc agctcactca cggctttcat tactgcttca
    12241 cgcgtatcgg cactgaccag cgtgctgtta ttgagcaccc gggaaaccgt tgccacagag
    12301 acgccagcct gacgcgctac atcacgaatg gtgatcatat tcactacctg ttatgagatt
    12361 gcagtaaatg actgctcgct ggcggctatt ttgtcagcac tgaatacagg acttcgtgaa
    12421 tcgagtcaca gcaatggaaa cggttacagc cgttttgtta acgaatgtga tccagatcgt
    12481 tatcttgatg ttttagatat tgtcggtccg gcagcacgca aagttaactg acgccagagc
    12541 cattccaccg gcccctggcg gaagtaacgc agccagataa cagagaagaa gatattcgcc
    12601 aaccataccg gaataacaaa cgccagcagc tccagacggt caaactgcat aaacaaaccg
    12661 aggtggtaaa aaagcgtagt acaaatcagc gtttgcaata gatagttggt cagtgccatc
    12721 cgtccgacac aggcgattgc cagcaccagc ttaaagcggc ttaattgcgg ccagaagcca
    12781 taaaacagcg acgcatagcc aatcgcctga aacggcgcac tcagttcccg cggcatctga
    12841 agtaagaagg cgcaccaacg atacgcccag tccagtcgcc attgcagggc gatggcagga
    12901 aggttaatga tcactccaat cgccaccagc acaaaaccag tacgacgata gtgacgaaag
    12961 ctgaactgcc ctttcagcca gccactgcgc atcaatgcgg caccaatgag catcatccct
    13021 gccagttgcc agccatactg tgcgcccagt gccagtaaac tgttgccaac accatcggca
    13081 cgattactga tcgcgtcaac accactgtga agtttccagt gcttttcata caaaatagcc
    13141 gatgcatcag gtgtccaggc gcggctggtc tggctatcgg aaatcaaccc caataacagc
    13201 aaaacgccaa gccccaccag ataaagcatg acgccggtat taaacaggct ttttaccgat
    13261 ggcgcatcgc gcaccagccg ccagcagatt aagcccacca gcccgtaagc cagcagaata
    13321 tcgccgtccc agaacaataa accgtgaata aagcccagca agactaacag cgttaaccgc
    13381 gactggatcc atcgtctgcc acggggcagc aacatttgca ggcccgcacc aaacaacagc
    13441 gcaaaaagcg tgaggaattt cacctggccg atgagatcga gaaatgccca ggtccatgca
    13501 tcctgtggcg taatagcgcc gtaccaggcg ggattgagat aagccgcctt tggtaaccca
    13561 aaggcgctga tgtttaatag caggatcccc agaatggcga cgccgcgaac aaaatcgagc
    13621 gtgacgttgc gctccacggt tcctgccttt ttatcagttg tgatgacgca cagcgcgcag
    13681 aaactcgtgg cgcgtattct gactggattt gaacaatcca ccaagagagg tcgttgtcgt
    13741 ggcactggtt gcatcgcgga tgccacgcgc cttcacgcag taatgcaccg cgtcgataga
    13801 gacagccacg ttattggtgc ccagcagcgt ttgtagcgca atgagaattt gctgcgtcag
    13861 acgttcttgt acctgcggac gctgggcaaa gaactgcaca atacggttaa tttttgacag
    13921 accgatgacc gaatctttcg ggatataggc caccgtcgct ttgccatcga tggtaacaaa
    13981 atggtgttca caggtgctgg tcagagtgat atcgcgcacg gtgaccattt catcgacctt
    14041 cattttgttt tcaatgaggg tgattttcgg gaaattggcg taatccagac cggagaaaat
    14101 ttcatcgaca tacattttag cgatgcgatg cggcgtttcc atcaaactgt catcagccag
    14161 gtcgagattc agcagctgca tgatttcggt catatgacca gcaataaggc ttttgcgcgt
    14221 ttcgttatcc atttcatgca cgggcgggcg cagcggtgtt tccagtcctc gcgcaactaa
    14281 cgcttcatga accagggccg cttctttact gagtgatggc atttatgatt tctcctgcag
    14341 gtgtgacgcc tccgccctgc gtgggggcaa agttattaag ctgatttaca gcctgattat
    14401 tgtgcgtgag gcggcgcaca taatccagta ttcacagcga taattattgt aattgccgct
    14461 gcctttcatc agcagatgtt aaaacatcgt tatgcaaata cggaagtgaa agttactcac
    14521 agcacattga ataaacggta tgatgaagaa attgcaaaca acacaacaag gagccacgca
    14581 tggaaatgct cgaagagcac cgctgttttg aaggctggca gcaacgctgg cgacacgact
    14641 ccagtacctt aaactgcccg atgacgttca gtatctttct cccaccacct cgtgatcaca
    14701 ccccgccacc agtgctgtac tggctttccg gattaacctg caatgacgag aacttcacca
    14761 ccaaggcggg tgcccagcgc gtggcggcgg aactggggat tgtactggtg atgccagaca
    14821 ccagcccgcg cggcgaacag gttgccaacg acgatggcta cgatttaggc cagggcgcgg
    14881 ggttttatct taacgccacg caaccaccgt gggcgacgca ttaccggatg tatgattatc
    14941 tgcgcgatga attaccggcg ctggttcagt cgcaatttaa tgtcagcgac cgctgcgcta
    15001 ttagcggtca ttccatgggt ggtcacggtg cgctgattat ggcgctgaaa aatccgggta
    15061 aatacaccag cgtttccgcc tttgcgccaa ttgtgaatcc gtgcagcgtc ccgtggggaa
    15121 tcaaagcgtt tagcacttat ttaggtgagg acaaaaatgc atggctggaa tgggacagtt
    15181 gcgcgttgat gtatgccagt aacgcgcagg atgcaatccc gacgcttatt gatcagggtg
    15241 ataatgatca gtttcttgct gaccaactgc aacctgcagt actggcagaa gccgcgcgcc
    15301 agaaagcgtg gccgatgacg ctgcgtattc agccgggata tgatcacagt tactacttca
    15361 tcgcctcttt tatagaggat cacctgcgct tccatgcgca gtatttactg aagtgaaagt
    15421 ccgcccggtt cgccgggcat cttctcatca gaagcgataa tccactgcca taaagtaacg
    15481 acgtccgtct tcgttatagc tgtagtcgtc acgactgaga tccttgtcgc caaggttcag
    15541 cacgcctgcg cgcagtttga cgtctttagt cacctgccag gccgcgccgg tattccagat
    15601 ggtataaccg cccggtgttt tagccgtcgc actgtcggcg cgtttctgcc cggtatagtg
    15661 accagaaaca tagaatgacc agtcttccag cgccagcggt ttccagtcca gcgtaccgtt
    15721 agcagtatgg aacggcagat cggatagcgg tttgttttcg ccgttgctga catcacgacc
    15781 atcgttgtag gtgtagttga tcgacagttt ccattcatcg ttgaacggaa ttttcagttc
    15841 ggtttccacg ccctgaatac gagctttgtt aacgttgtag tagctaaata ccggtatgcg
    15901 ccgtccgtta gcgcccgtct caaaaccgac aaagttttgg tagcccggtg cagcattgac
    15961 gtcagacgta cggctaatgc tgatacgatc tttcacatcg ttacggaaaa cggtaacgct
    16021 ggattcaacc ccttccagcc agccttcttc acccatgtag taaagcccca gctcccaact
    16081 ttcgctggtt tctggtttca gatccgggct acccacaatc ttacatgcgc cacggcagga
    16141 attgctcgtc cagtcagggc taagttgcaa cagagaaggg gctttaaatg ccgtcgccca
    16201 gccccctttc accgttacgg tgtcggtggc gttataaacc aggtaggcac gcggactcca
    16261 gtgttcaccg taggtttcgt gatcgtccat acgcacgccg gtcgtcagcg ccagcggctc
    16321 gaagatccgc cattcatctt ccacaaacag cgcgtactgg ctggcagacg ttttggagct
    16381 ggttcccccg gtcaggttca ccgcatcgct aagtttgtcg tgacgccatt caccgccaac
    16441 cgtgagaaac tgattaatcg ccgtcagcgg caacgtgtat ttgccgtcga ccgtattgct
    16501 ttcggaagtt atcgggctgc tgttgccagg gtttttgttc tcgactttct caccgtagta
    16561 tttcagttcg ctggtgccgt aatcccaacg cccattatgg ctgacggagt agttctggcg
    16621 ttccaggcgg tttttgtcca gcgagtcgga atcacgatcc tgacggtcga aaccgtatcc
    16681 ggcagtaaaa tcgtgatttt gattcggtgt ccaggcaaat tcgacattgc cgtcgcggct
    16741 ggagaatcct tcaatacgcg gcgtttctcc ggtatcggtg gtcgttgagt tttgcgggtc
    16801 atccttttca cgttttgcca ggctgccgta agctttcatt cccagcacgc cgtcaattaa
    16861 tggtccgctg gtaaagaatt gaccgttata ggtatcaccg cgatcgcgat gttcctgaac
    16921 ggtagtatcg acggtaacgg tgcccgacca tttctgaccg atttttttgg tgatgatatt
    16981 cactacaccg ccgagcgcat cggaaccgta cagcgacgac atcgggccac ggaccacttc
    17041 aatacgctcg atggaatcga ccgggatcca gttcagatcg aaatcattgt ggcggaagac
    17101 ggcattgcgg gagttaacgc gtttaccgtc gacaagaatc agggtgtagc tgctgtccag
    17161 accacgaata cttacgccct tacggttatc cccttcgttc gtcagttgta cgccaggcac
    17221 ttctttgagg acatccttca gattctgtac cggttttcgc tgcaggtctt cctgggtaat
    17281 gacgctgata ctggcgggag catctttaag gttttgttca acggaagatg cagtgacaac
    17341 catcgtttcg ccatcatcat cgaccgctaa cacaggccat gcacaagaaa tagcggacaa
    17401 acacagcccg acccgtacga aagggttcaa cctaaacatt ccatatctcc atgaggtaac
    17461 tacgaaaata aaatgggtat cgctcacatc ttctttacgt ccccttgcgt acggcagcat
    17521 cgcggtggac ttatttgtat gcaggtgatc atcccagaaa gccggaacag cctctgattt
    17581 gtcagccttt tttgattgcg gcgtaacgat aatgcaaacg ataacaatta tcaatccaaa
    17641 tgttaaattt tatatccgca tgatttgtca gggaaatgga attgaaaaag ccctctcggt
    17701 tgagagggct tagcaaggaa gcgaggaaac ttatttctta tcgttctgcg ggaacttcat
    17761 ttcgctgtag cgtacgaagt gagttccttt aatcagcttg tagccgaacc aaataatcag
    17821 gaacagcggg ataccaatat acgttgccgc tacgccgccc cagtcaatgg tatctttcag
    17881 gaacgcttcg tagttctggc ccaaagtgat aatcagacac agaatgaatg cgaagatcgg
    17941 ccccagtggg aagaaacctg aacggtacgg cagatcgtta atgtcgtgtc cctgcaatac
    18001 gtaaccgcga cggaagcgat agtggctaat ggcaatcccc agccaggcga taaaacccgt
    18061 catcccggag gtgttcagca gccacaggta taccgtctgg ttgccaaaca tggaggtcag
    18121 gaagcacaaa ccggcaatca ccgtcgtcgc atacagcgca ttacgcggca cgccaccacg
    18181 cgacagttta gcgaaaatgc gcggcgcttt accgtcacac gccagggtat acagcatacg
    18241 agtagacgca tacataccgg agttacctgc tgacagcacc gccgtcagaa taactgcgtt
    18301 catcaccgcc gccgcagaga gcagacccgc gtgctggaac accagggtga acggactgac
    18361 gctgatgtct ttaacatcgt tacgcagcag gctcggatcg gtatacggaa taatcaggct
    18421 gataatcagg atcgcgaaca catagaacaa caggattcgc cagaatacct gacgtaccgc
    18481 gcgtggaatg tttttcgccg gatcttcgga ctcgcctgca gcaataccga tcagctcggt
    18541 tccctggaaa gagaagccga caatcatagc tacgccgatc atcgccgcaa aaccaccagc
    18601 aaacggcgct tcgccgattg tccagttgct ccagcctgca ggctgcgcgc ctttgaagat
    18661 accgataatc atcagcacgc caacgatgat aaagacaata actgtcgtga ctttgatcag
    18721 tgagaaccag tattccgctt caccaaagcc gcgaacagag atgtagttca acaggaagat
    18781 aacgccgagg aacaacgcac tccagatcca gcccggtgta tccgggaacc accagctcat
    18841 gaccagctgt gctgcaacca ggtcaacggc aatagttacc gcccagttgt accagtagtt
    18901 ccagcccagc gcgaagccaa agccttcttc aacatagttc tgaccgtaag tggcaaacga
    18961 accggaaacc ggcatatatg cagccagttc accgagactg gtcatcagga agtaaaccat
    19021 caggccaatc agcatatacg agagcaatgc cccgcccgga cctgcctgag aaatcgttgc
    19081 gccagaggca acaaaaagac ctgtaccgat ggaaccgcca atggcaatca tcgtcaggtg
    19141 acgcgccttc aattcacggc gtaagcccgg cgcttctgta gttttagttt cggaacccat
    19201 acaaaaatgc tatccatctt aaaaatgagg cgcgattgta gcagacgatt ggcaatcctt
    19261 ccggcagaaa tacacggtta taagacacct tcatgatcgc ccagggatta taagtaaagc
    19321 agccaatctc ttttctggcg aatgcatgct gagggcgctg tgtcacgaaa tcgacacagc
    19381 accagcatgt tcttgtacag caccttaacg cggtacattc gcgggatcgc aatagtccag
    19441 aaagcgccgt agcgcgttgg aaaggtgttt ttgccgatga tgtatacgcc acaacgtacg
    19501 catcaggcgc ggcagaggga ccgcaacttc acttaatgtg cctgcctgca attgatcttc
    19561 aatcacccgt cgcgacaggc aactaattcc caacccatga cgcaccgcat gtttgattgc
    19621 ctcggagtta cctaattcca tcgccatctc aaacttcggt aaatgtgaca gcaacagata
    19681 atcgacaatc tcccgcgtgc cggaaccgcg ttcacgcagg atccacggcg cagcggccag
    19741 ctgttctaag gtgaccggac cacgagccaa cggcgaagtc ggcgcggcga aaaccaccag
    19801 ctcgtcttcc agccacggtt cagaaatgat ttcagtgctg tggcacggtc cttcaataaa
    19861 gccaatatca acgcggaaat ccagcaccgc ttgcatcacg tcctggctat tcccaacgct
    19921 aagttcaatc ggcaactgcg gataatcatg gcgataacgg gcgataactg caggcagaat
    19981 gtagttaccg atggtactac tggcatagat acgaatcgcg ccgttgtctt cgcgaaacag
    20041 ttgttcgatt tcaaccgcct gttcaagcag tgccaacgca cgcggataga gcagccgccc
    20101 gtgttcatta acaaccagtc ttttccccac gcgatcaaac agttgaacgc caagctgccc
    20161 ttccaggtcg gtcaaggctg cgctcactgc tgattgcgac aacgccagca tcaccgacgc
    20221 ctgggtggtt gatccacttt tcaatacttc tgcaaaaact tccaactgcc ggagggtgat
    20281 gtgcatagtc gcttaccact tataaagatt aattataaat atataatcaa ttttattttt
    20341 aaaccagtta gtcgtaacct tatgcccggt taaaggagag ggttatgacg aatatcacct
    20401 tacagaaaca acatcgtaca ctgtggcatt ttattccggg gttagccctg agtgcagtta
    20461 tcaccggggt cgccctgtgg ggtggttcca ttcccgctgt tgcgggtgcc gggtttagtg
    20521 ccctcaccct cgcaatcttg ttggggatgg ttttaggcaa caccatttac ccgcacatct
    20581 ggaaaagctg tgacggtggt gtgctgtttg ccaagcaata tttattacgt ctgggtatca
    20641 ttctttatgg cttccgtctg acgttctcgc aaattgccga tgtcggtatc agtgggatca
    20701 tcattgacgt gttgacgctg tccagtacct tcctgctggc ttgcttcctg gggcagaaag
    20761 tgtttggtct ggataagcac accagctggt tgatcggtgc cggtagcagt atctgtggtg
    20821 ctgccgcggt actggcgact gagccggtag taaaagcaga agccagtaaa gtaaccgtgg
    20881 ctgttgcaac cgttgttatc ttcgggaccg tcgcgatttt cctctacccg gcgatatatc
    20941 cgctgatgtc tcaatggttt agtccggaaa ctttcggtat ctacattggt tctactgtgc
    21001 acgaagtggc gcaggtggtg gcggcaggtc atgccatcag cccggatgca gaaaacgcag
    21061 cagttatttc caaaatgctg cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg
    21121 cgcgtgttaa acagctgtct ggggcgaaca gcggcgagaa aagcaaaatc actattccgt
    21181 ggtttgccat cttgttcatc gtcgttgcca tctttaactc gttccacctg ttaccgcaga
    21241 gcgtggtgaa catgctggta acgctggata ccttcctgct ggcaatggcg atggcggcgc
    21301 tgggtctgac cactcacgtc agcgcgctga aaaaagccgg ggcgaaacct ctgctgatgg
    21361 cactggtact gtttgcctgg ctgattgttg gtggtggtgc gattaactat gtgattcaaa
    21421 gcgtcatcgc ataaaccact acatcttgct cctgttaacc cgctatcatt accgttttcc
    21481 tccagcgggt ttaacaggag tcctcgcatg aaatacattg gagcgcacgt tagtgctgct
    21541 ggcggtctgg caaatgccgc aattcgcgcc gccgaaatcg acgcaaccgc gtttgccttg
    21601 ttcaccaaaa accaacgtca gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa
    21661 ttcaaagccg cctgtgaaaa atatcactac acatcggcgc aaattcttcc ccacgacagt
    21721 tatctgatta acctcggaca tccggttgct gaagctctgg aaaaatcgcg cgatgccttt
    21781 atagatgaaa tgcagcgttg cgaacaactg ggactttctt tgctcaactt ccaccctggc
    21841 agccatctga tgcagatttc agaagaggat tgccttgcgc gtattgccga atccatcaac
    21901 attgcgctgg ataaaactca aggtgtcacc gcggtgattg aaaacaccgc cggtcagggc
    21961 agtaacttag ggtttaaatt cgaacatctc gcagcgatta tcgacggcgt ggaagataaa
    22021 tcccgcgtcg gcgtctgcat tgatacctgc catgctttcg ctgccgggta tgatttgcgt
    22081 actcctgccg aatgcgagaa aacgttcgct gattttgccg gtattgttgg ctttaagtat
    22141 ctgcgcggga tgcacctcaa cgatgcgaaa agcacctttg gcagccgcgt tgaccgtcat
    22201 catagcctcg gcgaaggcaa tatcggtcat gatgcgttcc gctggatcat gcaagacgac
    22261 cgtttcgacg gcattccgct gatccttgaa accatcaacc cggatatctg ggcagaagag
    22321 atcgcctggc tgaaagcgca acaaactgaa aaagcggtgg cctgatcgat gaacgacagg
    22381 gaaaagcaga ttcttaaaat tttacggcgt aacccgctga ttcaacagaa tgaaatcgcc
    22441 gatattctgc aaatcagccg ctcacgtgtt gccgctcata ttatggattt gatgcgcaag
    22501 ggtttgatta aaggcaaagg atatatcctt accgagcagg attactgtgt ggttgtgggt
    22561 gcaatcaata tggatattcg cggaatggcg gatatccgtt acccgcaagc agcctctcat
    22621 ccaggttctg ttcactgttc cgcaggcggc gtcggacgta atatcgcgca taacctggct
    22681 ctgcttggtc gggatgtgca tttaatttca gctattggca acgattttta cggcgagaca
    22741 ttactggaag aaacgcgccg cgcgggcgtg aacgtttcaa actgcattcg tctgcatgga
    22801 cacagcactg caacttatct cgctatcgct aataagcagg aagaaactat cctggcaatc
    22861 aatgacacac atattctgca acagctaacg cctcaactgc tcaatacttc acgggacttg
    22921 attcgccatg ctggtgtggt gctggctgat tgtaatctca cacctgaagc cctggagtgg
    22981 gtctttacca ttgctgatga aattccgatg tttgtcgata ccgtttccga atttaaagcg
    23041 aacaaagtaa aaaactggta cagccggatc cacactctga aaccgacaca aaatgaactg
    23101 gaaattttgt gggggcaacc gattaaagac gacaacgatc gcatcagagc tgtaaattcg
    23161 ctgcatcaac aaggtgttaa acgcattttt gtttatctga aagatgaatc cgtattttgc
    23221 agcgataagg atggagaaca atttttgctg accgcgccaa cgcatacgac agtagacagt
    23281 tttggtgctg acgatggttt tatggcgggc ctggtatata gctttcttga aggatacagt
    23341 ttccgcgaca gcgcccattt tgcgatagcc tgcgcggcaa tttcgcgcgc cagcggcagc
    23401 ttaaacaacc ctaccctgtc tgccgataac gcgctttcat tagtgccaat ggtgtaacaa
    23461 tgttgccgga ttcgacgctt gaccgcatct tatccggctc ataaattgcg cactacgcta
    23521 aaccaataaa gaatcctgca atagtagcgc tcatcaggtt actgacagca aaaacgcaat
    23581 agccagtaat accaccattc ccagaacact tctcatgaca tccatagtta ttatctcttc
    23641 atgtcatttt gggcgacggc tctctgccca tcataaatag ccgggcctgc atcttgcgat
    23701 actgacccgg cttgttatca acacttaatg ggtttttatg tagccgcgta cgcactcttc
    23761 gactaatccc cagaaccagt cggtatcaat agtgatgccg actttggtat tggcgggctt
    23821 gccaagaaca cccagctcgt cgcagacggt acgcccatag caagggccac tgttaacgtc
    23881 cacttcgacg tacatttcct gggttttaat gccatcaggg ttaatcaaat aaccgatgca
    23941 ggtggcgtcg tgcaccgggc cgccagccag gccgtagttt tcgaactgcg ttttgagagt
    24001 gaagttcatg atgtcgctga acagctctcc ggcagggccg ccagcccttt ccatccgagc
    24061 aatcacatcc ggggtgcaaa cggtctggtt ggtaagatcg aggcccatca tcactaatgg
    24121 aacgccggag gtgaacacca cacgtgcggc ctccgggtca gcaaagatgt tgaactcagc
    24181 agatggggtg aagttgccag taccataagc accgcccatc agaacaattt cacggatttt
    24241 cggcaggatc gcgggttgca tacgcattgc caccgcgatg tttgacaacg gaccaaccgg
    24301 caccagagtg atatcgccat cgcttgccat aagggtatcg atgatatatt tcaccgcatg
    24361 agtgttttct gcctggcggg tcagcggctc gaataccggg ccatccagtc cggtttcacc
    24421 gtgaatatta tcggcaacaa tttgttgacg cataatgggc tgcggcatcc ccgcataaac
    24481 cggaacatta atctccagtt tctggcaaac attcaggcca ttaattaatg ttttatcgag
    24541 ggtttgatta cccgctacaa tggtgatacc taataaatca atcgccggat gtttcgccgc
    24601 catcattata gcaatagcat catcatgacc cggatcacaa tccagaataa tttttctctt
    24661 ttccattgtt tatttcctct gtttccagtt gcgttattgt ttctacagca aagaatattc
    24721 gacctggccg atgattgaat cttaacaaca gcgtacgtat gctaaatatg agaaatctca
    24781 tagcggataa acatcgtgaa agaaatccac aataatgatc ttaagcagca attgatgagt
    24841 gaatctgcgt ttaaggattg cttttcaacg gatgtttcag ccgatacgcg gctgtttcat
    24901 tttttagcgc gtgactacat tgtgcaggaa gggcaacagc cgtcctggct gttttacctg
    24961 acgcgaggcc gcgccaggct ttatgtcacg cttgctaatg gtcgcgtgtc gctgatcgat
    25021 ttctttgccg cgccctgttt tattggcgag attgagttaa tcgacaaaga ccatgaacca
    25081 cgtgcagtgc aggctattga agagtgttgg tgccttgcgc tccctatgaa gcattaccgc
    25141 ccgttgttat taaacgacac gctattttta cgaaaactct gcgtcacctt aagtcataaa
    25201 aattatcgta atattgtttc tttaactcag aatcaatcat ttccgttagt gaatcgcctg
    25261 gcagctttta tattactctc gcaggaaggt gatctttatc acgaaaagca tacgcaagcg
    25321 gcagagtatt taggcgtttc ttatcgacat cttttatatg ttcttgcgca attcattaat
    25381 gacggtttat taattaaaag caagaaaggg tatctcatta aaaacagaaa gcagttgtca
    25441 ggactggcgc tggagatgga cccggagaat aaattctccg ggatgatgca gtaaaaatta
    25501 ttggcaatag tgcgattgcc ggatgcaacg cttaataagg cgtacatccg gcaatgaaac
    25561 tcaagctaaa ccaataaaga acccggcaat ggtcgcgctc atcaggttgg aaagcgtcgc
    25621 cgccgccagc gcccgtaaac caagctgggc gatttccggc gcgcgttttg gcgaaatagc
    25681 cgaaaacgct ccaacgacaa caccgataga accaaagttt gcaaacccac agagggcgaa
    25741 cgagataatg gcaatggtct tgacatccag cgtgccgctc gtttgcagat agggcgagaa
    25801 attgagataa gcaacaaact cattaatcgc cagtttctgc ccaatcaaac ttccggcaag
    25861 atttgcatca ctccagtcca cccccataat ccatgccaac ggggccagca catagccgaa
    25921 aatgctttcc agagagacat tggcaaaacc aaaccagccg ccaactccac caataatgcc
    25981 gttgatcagc gcaatgatcg ccacaaatgc cattaccacc gtcgccacac cggcggcgat
    26041 tttcagcccg gtcattgcac cgttcgccgc agcttcaata atgctttttg gcggtgtttc
    26101 gctaaacgac agattttcaa acgtaacttg cgaaggttcc gtcgccggac taagaatacg
    26161 tgcaaacagg ataccgccag gaatagccat cagcgaagcg gctaacaaat agtcaatcgg
    26221 cacgcccatt ccggcatagc caatcatcat cgaacccgca atagacgcca tgccgctaca
    26281 aatagccgta aatagctcgt tgcgattcat gcgatcaata aaaggtttaa cgatcgccgg
    26341 gatctcattt tgcccgagga aaatagtggt gactgcgaca aaagattcga ttttgctgat
    26401 gttgagggct ttctgaaaaa tactgccaag aatacgaatc agcagcccca ttacgccaat
    26461 gtagtacagc aagctgataa gcgcggtaac gaaaataatc gccgggagta cgcgaaaggc
    26521 gaagataaag cccgcaccgt caaacagaac atccattttg ggaccgacca gcgaaccaaa
    26581 aataaaggca cttcctgcgt cactgtagga catcacttta tggacgccta atgccgcctg
    26641 ctctactgcc cattttcccg ggggaaaata gagcatgatg ccaccaatgg caatttgcag
    26701 cagcagcgca gccccaaccg tgcgcaagct gatgctcttt ttatttaccg acaacagaaa
    26761 tgctattgcc agtaacacca ccatccccac aacacttctc attatatcca taatgatttt
    26821 cccttcatgc cagtaaaccc ggcgtcagcg ccaggttttg gtatgtttga tgagtacggg
    26881 cgacggcttt ctgcccacca gaaattaacc cacgagacgc tgatattctt tggcaatttc
    26941 gctcgccaga atggcgttat taaagaccag ttgaatattg gatttcaggc tatcaccgcc
    27001 ggttagctcc gcaacacgcg ccagcaggaa cggtgtactc tctttcccaa taacaccctg
    27061 ttcttcggct tcagcaacgg cttgatcaat tgctgcattg atggtttctt ctggcatcgc
    27121 aaactgttcc gggatcgggt tcgctaccac caggccgccg ttcaaaccgg tttgccactt
    27181 cactgccatc gcgcgagcaa tttcgcgggc actgtcaaga cgaatgctga cttcaaatgg
    27241 gctggttcgg cagaaaaacg caggcagcgc ttttgtctga taaccaatta acggtacacc
    27301 gaaggtttct aaatattctg ttgttaatcc taaatcgaga atagattttg cccccgcaca
    27361 aacaacagtg acattggtat ttgccagttc ctgtaaatcg gcagaaatat cgaaggtatg
    27421 ttccgctccg cgatgcacac caccaatacc tccggttgca aaaactttaa tcccagccag
    27481 tgctgcaata atcatggttg atgcaacggt agtcgcgcca tttttccctg cagcaacgac
    27541 aaaaggtaaa tcacgacgac taactttcgt cacgttatgt ccttcacggc ccagcaattc
    27601 aatttcttct ttacttaagc ccactttcat cacgccgcca ataatggcga tagtggctgg
    27661 tactgcgccc tgtttacgaa ttgtttcttc tacttcaatt gcggtttggg cattttgtgg
    27721 gaatggcatt ccgtgagaaa ttatggtcga ttccagcgca ataaccggtt ttttattttt
    27781 taaagcatcc tgtacttcag cggatatttg taataattca ggggacagag ttaattcaga
    27841 cattctgtgt tctccactaa cgatgtaaca ttggcaattg ataattcagg gttgttggta
    27901 tattcacagg caagtgccat tgacgaacat ccctgcgcaa atcgaacaga atcgataaac
    27961 ggcataccgt ctacccaaca ggaggccagc cccgccatca tggcgtcgcc tgccccagta
    28021 acattgatga cattcgtttt aataggggcc gaccacccac tttcaccgtt gatatcactg
    28081 tagtaaacgc catcccctcc catgctcagc accaggcggt tcaggccgtg ttgatgaaac
    28141 caggcggcga cctttgcgac atcttcacgt ccggaaagtg caattccgct caatgtttcg
    28201 gcttcaagac ggttaggttt taacgtatgg attttgctca gatgatcgcg gattttgacg
    28261 catttccatg ccgataccgg atcaataaat acaggcgttt ccccgctatt ttccagaacc
    28321 cagaccagcg cctcttcact aaggttgcaa tccgcgacaa taacctttgc accgcgaata
    28381 aactcctgat gctgggcaag aaattctgca gaaatacagt cactaatact catatcattt
    28441 atcgcgacaa gcatttctcc tgtattatcg agcagtgata aatagctgga tgtattctca
    28501 cccggaacaa ttaagcattt atcgacataa acaccggatt gattagtttg cgccagtaat
    28561 gattgcccat aaaaatcacc acctactgca cttaaaagcc aggcattttt ccccagtaac
    28621 gccagattat gagcgatatt ccttcccacg ccacctgggg taaatttaat ttttccggga
    28681 ttcgaatccg cataattcaa tggatgatgt gaatacccag ccacatcaat attcgccgag
    28741 ccaataatta ctatgtagtc cttttcgcgc atagcacgcc ccctggcaat aaattgccaa
    28801 cgaagaaatt gggtaaaata gtcattacag tcaataagac tattagagca cgtgttcaga
    28861 tttaaacaca tgttcataat aagcatgttt aacgtaatgt aaatcgatca gaaaaaacga
    28921 aatcggccag atatgaaggc ggtcacaaag tttgtctatt tatgcagcaa caaaagagaa
    28981 agttgtgatg tgtaacacac aatatggcag gcaagaaaaa gggcagagat gtttctgccc
    29041 tcagattgtt gacaacgcgc gctttgttca tgctggatgt ggcgtaaacg ccttatccga
    29101 cctacaaaag cgcaaaaatt caatatattg cagggttcat gtaggtctga taagcatagc
    29161 gcatcaggca attttgcgtt tgtcatcatc ggggcagaga tgtctcagcc ccgatgatga
    29221 agagaaatta tgcgtttttt tctactattt gtgtttccgg gcgtttgagg aaggcatagg
    29281 ccaaacccgc caccagcgta ccggcaataa ttgctaccag gtaacccaat accggcgtaa
    29341 tagcgccagg gatcagcaga acaaacagac caccgtgtgg tgccatcagt ttcgcaccaa
    29401 tcgccattga aattgcgcca gtcagcgccc caccaacgat acagcacggt agcacacgca
    29461 tcggatcacg agcagcaaac ggaattgcac cttccgaaat aaagcacaga ccaagtacca
    29521 gagcggcttt gccaccttcc tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg
    29581 ccagacccat tgccagcggt ggcaccatac ctgccgccat aatcgccgcc atcgggccat
    29641 aggtttgagt actcagcaga cccacaccga atgcgtacgc tgctttgttt accggaccgc
    29701 ccatgtcagt acacatcatg ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc
    29761 ccatggtctg cagccagtga gtcaacccat cgagaatgcc agcaaccggt ttaccgatta
    29821 ggtagatcat cgccagaccg accaccagac tggaaattag cggaatgatc aggatcggtt
    29881 tcagtgcctc catactctgt ggcagtttca gttgcgtact gattaacttc gcaatgtaac
    29941 ccgccaggaa gcccgcaata ataccaccaa tgaagccaga accggtgcta accgccagca
    30001 taccgccaat caggcccggc gtgagacccg gacgatcggc aatggagaag gcaatataac
    30061 ctgccagtac cggcaccatc agcgcaaagg ctgaaccacc accaatctgc atcagcgccg
    30121 ctgccaacgt acccggctct ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac
    30181 acagaccacc tgcaacaacc atcggcagca tgtaagagac gcccgtcagc aagtgacggt
    30241 aagcgcctgc accctctttc ttaccttcag tggtcgctgt ttgagctttg cccgctggtt
    30301 catacggcgt tgcttcagca accgctttat ccagttcctg cgcagttttc ttcagcgcca
    30361 gaccggtaga ggtgcgatac atcggtttac cagcaaattt cgccagatcc acttcgatat
    30421 ctgccgccac aatcaccaga tccgctgctg cgacttcttc aggggtgatt gcattgcccg
    30481 cgccaacaga accacgggtt tcaactttca cccaccagcc acgttttttc gcttcggttt
    30541 caatggcttc agccgccata aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa
    30601 ctacgcgttt cggaccgctg gcagcaaccg gtgctgtcgc agcgaccggc gcagtgtaag
    30661 gtttcgcatg gcctttggct tcactcagga acagctcagg gtgcgcaact gcccgggaaa
    30721 tatcacccag ccagacattt ttaccgttca gcgcgctgtc attcgggatg gaatcaccga
    30781 gaacaatcgc catttctgcg tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg
    30841 ccgccgcgcc cagcagggtc ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa
    30901 taatcagcag cgttttcatt atgcctctcc tgctgtcagt taaaaggttg taagtcgacg
    30961 cgcgccatca ttgcggccaa ctgcggacga tcggtaatac ccacattgct ttgacttacc
    31021 gccagggctg caacagctgt cgccagacgc agtgtgtgtt cactggattc acgcatcagc
    31081 aagccataaa tcaggccacc aaccatagaa tcccctgcgc caacggtgct tacgacatcg
    31141 actgacggtg gtttggcgat ccattcgccg gaggcattaa cccaaagcgc gccttcggca
    31201 cccagtgaaa taacaacatg cgcaatacct tgttcacgca gcgcatgcgc agcttcaatc
    31261 acatctttca tttcaggcag tttacggcct gcccagattt ccagctcgcg gcggttaggt
    31321 ttcaccagcc acggtgccgc tttcaaacct gctactaacg cttcacggct actatcaaag
    31381 ataatgcaag gacactgact acgcaggcga gtcatccagt cggtgaacgc ttccgggctg
    31441 acgcctgacg gtaagcttcc gctgacacag accatatcga actgaccgag ccagctcaga
    31501 gaatcagtca caaagcgttc ccagtcggcg ggggtgactt caaaacccga gaagttgaag
    31561 tcggtcactt cgccgtcttt ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca
    31621 acctggaaac ggttggcaat gcccagctcg ctgaacagtt gctgaaaacc atcctgattg
    31681 tctttaccga ggaagccgcc aacggtgaca tcaattccca ggtcttttaa tactttggcc
    31741 acgttgatgc ctttacccgc cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg
    31801 cgttcaattt ccgggcagaa accaacaagg tcataagccg gattaagggt gatagtagca
    31861 acacgtctgc tcattatgcg ccctccccaa gaccagcagc gatagcgtcg ccaatggctt
    31921 tcagcgcctg ttcagcatct gccccctggg cggtaaagcg taggcgatga cctttcttaa
    31981 cgccaagtgc cacaactttc atcagactac gtccgtttgc cggtttgccg gtgccatcaa
    32041 ggtttgtcac ggtaatatca ctgttaaatt gtttaatggt attgaccagc atggtacctg
    32101 gacgagcatg caggccgtgt tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg
    32161 tcagcgcatc atcgctggtc agcagcgcca gcaacgttgc cgcatccgct ttcagcaagc
    32221 ggtcagcttt attgtcgagt aacaaatcag cgagacgctt aagaaccgca atgggctgat
    32281 cgtcattcat cgccacactc accagcatgg ctgccgtttc gccgtccaca tcaaaagcat
    32341 ttgccgcacg gcttaccgca atcgcgctac gcagattgcc ttcggcgcta tcgctcagcc
    32401 agataccctg tccgaggttc agcggttgtt cattgatggc tttggtgacg aaagtggcgt
    32461 caactgcccc cgcctctttc agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc
    32521 tggcgacgat atccagcgtc agcatttcgt tgtcgagctt cagctgctca ctctgctttt
    32581 cgcccatcag caatgcgcga agttcttctg ctgttgttgc tgacttcagt tgttcagcaa
    32641 cggaatcatc gctcagtacg tgggtcagct ggcgtagcag gcccagatgt tcatccgagc
    32701 tggcagcaat accgatcgcc acgtacgcta cctggccgtc accccaggtg acgccttccg
    32761 ggaactgaaa tacctgaacg ccggttttca gcacctgatc gcgggtgtcg gtagtgccgt
    32821 gtggaatagc aataccattg ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc
    32881 cattgacgta gccttcagct acattaccgg cctgcaccag cgccgcagcg acctggcgaa
    32941 tcgcctcttc tttgtctccg gccttttcgc ccggatggat gtcctgtatg gataactgga
    33001 acatagttct cctctcttgc tgaattgaaa cgattcagct tctatgagaa aaaaagcgcc
    33061 aacctggctt agggttaaag acaagatcgc gctgaaacgt ttcaagaaag cataatactt
    33121 ttgtttcagc acgcaaggaa agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa
    33181 ttttttgtca tttttcctaa ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag
    33241 cagcgtcagc agccaatcag caacagtgaa atggcatttt gattattcgt ggcgcttttg
    33301 atttcgctgt ctgtttattt tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt
    33361 gatcgaccag catgcataac tcccccgcag tcaccagcgc gaaatcgttt gacctgacct
    33421 cgacggcgtt tttaatcgtt gcctttctca ccggtattgc gggcgctctg caaaccccga
    33481 cactcagtat ttttcttact gatgaagtac atgcccgtcc ggcgatggtg ggattcttct
    33541 ttaccggcag cgcagtcatt gggattctgg tgagtcagtt tctcgccggg cgctctgata
    33601 agcgcggcga tcgcaaatcg ctgattgtct tttgctgcct gttaggcgtg ctgacctgca
    33661 ccctttttgc ctggaatcgc aactactttg ttttgctatt cgttggcgtc tttcttagca
    33721 gctttggctc aaccgctaac ccgcaaatgt ttgcccttgc ccgtgaacat gctgacaaaa
    33781 ccggacgtga ggcggtgatg ttcagctctt ttttacgcgc tcaggtttca ctggcatggg
    33841 tcattggccc accgctggct tatgccttag cgatgggttt cagctttacg gtaatgtatc
    33901 tgagcgcagc ggtagcgttt attgtttgcg gcgtgatggt gtggctgttt ttgccgtcga
    33961 tgcaaaaaga gcttccgctg gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg
    34021 atacgttgct gctgtttgtc atttgtacat tgatgtgggg ctcgaacagc ctgtacatca
    34081 tcaacatgcc gctatttatt atcaacgaac tgcatcttcc cgagaaactg gccggtgtga
    34141 tgatggggac cgccgccgag ctggaaatcc cgaccatgtt gatcgccgga tattttgcca
    34201 aacgtctggg taagcgcttc ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg
    34261 caggaatgct gatggcgcat tcacctgtca ttctgttggg cttgcagctg ctaaatgcta
    34321 tttttattgg cattctgggc ggcatcggga tgctctattt tcaggatttg atgcccggtc
    34381 aggcaggttc agccaccacg ctctatacca acacgtcgcg cgtgggctgg atcatcgcag
    34441 gatcagtggc gggcatcgtc gccgagatct ggaattatca cgctgtgttc tggtttgcga
    34501 tggtgatgat tatcgccact ctgttttgct tactgcgaat taaagatgtt taaggcgcgg
    34561 tcaacatttc cagatcgata agccaggtca ttgcctgctg acgcgagtta ccgcacattt
    34621 cagcgctggc ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg aaaattttac
    34681 agcgttgctg ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac
    34741 cgggaatagg gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt
    34801 ccatacgttg ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt
    34861 tttgcaccag caaaagtgcg aataccactt gcccgaaagg cccgtcgcga gtactttgtc
    34921 gcgatatttt tgacattttt gactacagga atttttcgat gccaagagcg aacgaaatta
    34981 aaaaaggtat ggtactgaat tacaacggca aactgctgct ggttaaggac attgatattc
    35041 agtcgcccac tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta
    35101 ccgggctgaa agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga
    35161 cccgccgtta cgttgacttc tcctatgtcg atggcaacga atatgtcttt atggataaag
    35221 aagactatac cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc
    35281 ctgagggcgg catgccggac atgcaggtgc tgacctggga cggtcaattg ctggcgcttg
    35341 agctgccgca gaccgttgat ctggaaatcg ttgaaactgc accaggcatc aaaggggcat
    35401 ctgccagcgc ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag
    35461 aatacttaag cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatgggtc
    35521 gtgctgacta acttcagccg cttgcagaaa agggatagct caggctgtcc cttttttaat
    35581 ttattatacc aatcttctat tttgcgcttc ctgaacacct tacgcccgca attttcactc
    35641 gttgattgat cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgttgt
    35701 catataactt tacactgtca ttgttaatta atcgttacta agacgtgact cctatgaata
    35761 caatcgcctc cattacgctc ccgcatcatg tacacgctcc acgctacgat cgacagcagt
    35821 tgcaatcacg tatcgttcat tttggctttg gagcctttca ccgcgcacat caggcgttac
    35881 tgaccgatcg tgtgctgaat gcccagggcg gcgactgggg gatctgtgag atcagcttgt
    35941 tcagcggcga tcaactgatg agccagctcc gcgcacagaa ccatttatat accgtgctgg
    36001 agaaaggggc ggacggcaat caggcgataa ttgtcggtgc cgttcacgaa tgcctgaatg
    36061 caaaactgga ttccttagcg gcaattattg agaagttttg cgagccacag gtggcaattg
    36121 tttccctgac gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca
    36181 ccagcaatcc gcggattatt cacgatctac aaacccctga agaacctcac tccgcgccag
    36241 gtattctcgt cgaagcactg aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc
    36301 tctcctgcga caatattccc gacaatggcc atgtggtgaa aaacgcggtg ctggggatgg
    36361 cggaaaaacg ttcaccagaa ctcgccgggt ggataaaaga acacgtcagt tttccgggaa
    36421 ccatggtcga ccgcattgtt ccggctgcaa ccaacgaatc actggcggaa ataagccagc
    36481 atctgggggt gaatgatccc tgcgcgatta gctgcgaacc gtttatccag tgggtggttg
    36541 aagataactt cgtcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg
    36601 atgtcctgcc atgggaagag atgaaactgc gtatgcttaa tggcagccac tcttttctcg
    36661 cttatctggg ttacctctca ggattcgccc atatcagtga ttgcatgcag gatcgcgcat
    36721 ttcgccatgc cgccagaaca ttaatgctgg atgagcaagc gccgacactg cggattaaag
    36781 atgtcgattt aacacaatat gcggataagt taattgcacg ttttgctaat ccggcactga
    36841 aacataagac ctggcaaatc gcgatggatg gcagccaaaa attaccgcaa cgcatgctgg
    36901 caggtattcg catacatctg gggcgcgaaa cggactggtc gttgctggca ttaggcgttg
    36961 caggctggat gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg
    37021 atccgcttag cgataaaatt cgtgaacttg ttgcgggcag cagcagtgaa caacgcgtaa
    37081 ccgccctgct ttccctgcgt gaaattttcg gtgatgatct gccagataac ccgcattttg
    37141 tgcaggccat cgaacaagcc tggcaacaaa tcgcacaatt cggcgcgcat caggcgctat
    37201 taaacaccct caaaatttaa cgatttctgc ggttaaagcg gatgaagctc gccttcgtcc
    37261 gctctcccct tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag
    37321 agtattgcgg agccgttgtg accagaacca acctcatcac cggttttctc ggcagcggga
    37381 aaactacgtc gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac
    37441 tggttaatga atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcac
    37501 tgctgaaaga gatccccggc ggttgcatgt gctgcgttaa tggtttaccc atgcaggtag
    37561 ggttgaatac cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc
    37621 tgggccatcc gaaacagatc ctcgatctct taaccgcacc cgtctatgaa ccgtggatag
    37681 atctgcgcgc cacgttgtgc attctcgatc cacgcctgtt gctggacgaa aaaagcgcca
    37741 gcaatgaaaa cttccgtgac caactggctg ccgcagacat tattgtcgcc aataaatccg
    37801 accgtgcgac gcccgaaagt gagcaagcgc tagagcgctg gtggcagcaa aatggtggcg
    37861 atcggcaatt aattcacagc gagcatggaa aaattgacgg tcatcttctg gatttgccgc
    37921 gtcgcaattt agccgagttg cccgccagcg ccgcgcattc tcatcagcat agcgtgaaaa
    37981 aagggttagc agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg
    38041 gacaagggca tcaggcctgt ggctggatat tcgacgctga tacggtattc gacaccattg
    38101 gtattctgga atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctacgtattc
    38161 ccgaagggct ggtgcgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg
    38221 ttgcgccacc ggacagtcgt attgagctga tttccagcag cgaagctgac tggaatgcct
    38281 tgcagagcgc gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt
    38341 cagtaagata attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat
    38401 tgtcggcctc gcgctgtttc tttcctggta tattcccatt aatcatggat tctggttgcc
    38461 gattgatgcg gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt
    38521 atggctggtt gcattgacca acaatcgcgc tttcgacggt tgttcactgc tggcgatggg
    38581 aatgttgatg ctgagtttct ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat
    38641 tattggtctg gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcactaat
    38701 tcctgtaaaa cgggccagcc caacgttgac ttttaccaat attaaccgcg tcagcgaact
    38761 gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc acggtatgat
    38821 gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc
    38881 ccttattatt tttgtggttt ttgcgtttcc cagagtaatg attggcgcac actggtttac
    38941 tgacatcatc gtcggttcca tgaccgtgat attgatcggt ttgccctggg tgttgctgac
    39001 gccattaagt gatcgattaa tcaccttttt tgacaaatca cttcctggaa aaaacaaaca
    39061 tttccaaaac aaataactca cagtaattaa catcatcagg tttattttta tagtgaggat
    39121 aatcctgatg atgcgcctcc tgctttcatc catcgagcgc aaaaatcatt ctctaagtaa
    39181 atgaatggat tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc cgctgattaa
    39241 ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca
    39301 atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt
    39361 tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt
    39421 ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac
    39481 aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga tccccgcgat
    39541 tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc
    39601 tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga
    39661 aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa
    39721 aggcgtacgc tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt
    39781 acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt atgaacagca
    39841 ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg
    39901 tgccggttca acgggacgcc atgtcggtat ttatatcggc aacaaccagt ttgtccatgc
    39961 ttccaccagc agtggtgtta ttatttccag catgaatgaa ccatactgga agaagcgtta
    40021 caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg
    40081 ctatcctgac gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc
    40141 agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta
    40201 aatgttgtcg taatttcagg aaattaacgg aatcatgttt atacgcgctc ccaattctgg
    40261 acgtaagctc ctgcttacct gcattgttgc aggcgtgatg attgcgatac tggtgagctg
    40321 ccttcagttt ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattaccga
    40381 cgtacaaaag tatctcgata cctattttgc cgaccttaaa tccactactg accggctcca
    40441 gccgctgacc ttagatacct gccagcaggc taaccccgaa ctgaccgccc gcgcggcgtt
    40501 tagcatgaat gttcgtacgt ttgtgctggt aaaagataaa aaaacattct gttcatctgc
    40561 aactggtgag atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa
    40621 cgtcgatatg gcaatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat
    40681 ctggtatcgc aaccctttgc tgaaaaatag cggcgtgttt gccgctctga atctcaacct
    40741 gacgccttca ctcttttata gttcacggca ggaagattac gatggcctag ccctcattat
    40801 tggtaatact gcgctatcta ccttttcttc acgtttgatg aacgttaatg aattaaccga
    40861 catgccggtc cgtgaaacta aaattgcggg cattcctctg actgttcggc tttatgcaga
    40921 tgactggaca tggaacgatg tgtggtacgc atttttactg ggtggcatga gtggaacttt
    40981 cgttggactt ctctgctatt acctgatgag tgtgcgtatg cgcccaggca gagaaatcat
    41041 gaccgccatc aagcgcgaac aattttacgt ggtgtatcaa ccggtggttg atacacaagc
    41101 tttgcgggta acgggcctgg aagtactgct acgctggcgg catccagtag caggtgaaat
    41161 ccccccggat gccttcatta actttgccga agcgcaaaag atgattgtgc cactgactca
    41221 gcacctgttt gagttgattg cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg
    41281 cgtcaaattt ggcattaaca ttgcgccggc ccacttgcac agcgaaagct ttaaagcgga
    41341 tatccagaaa ctgctcactt ccctgcccgc acaccatttc cagattgtgc tggaaattac
    41401 cgagcgcgat atgctgaaag agcgagaagc cacacaactc ttcgcctggc tgcattcggt
    41461 cggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga
    41521 gcgttttacg ctcgattatc tgaaaattga tcgtggattt atcaacgcca tcggtacgga
    41581 aacgatcact tcacccgtac ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct
    41641 gacagttgct gaaggggtcg aaacgccaga acaggcacga tggctaagcg aacgcggcgt
    41701 taatttcatg caaggctact ggattagtcg cccgttaccg ctggacgatt ttgttcgctg
    41761 gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag
    41821 tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt
    41881 gcgcatactg ctgctgttta tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa
    41941 ggaaagctat gcctttgccg tactgggcga accccggtac gcatttaatt tcaaccattt
    42001 tgattatgtg aaccccgccg cgccaaaagg tgggcaaata acgttgtccg ccctcggcac
    42061 cttcgataat ttcaaccgct atgcactacg cggcaacccg ggcgcacgca ccgagcagct
    42121 gtacgacacg ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc
    42181 tgaaagcgca cgctatgctg acgattattc ctgggtggag gtcgctatta atccgcgcgc
    42241 ccgttttcat gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt
    42301 tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat
    42361 tgcgccgtta accgtgcgca ttgagttagc taaacccggc aaagaagata tgctgagtct
    42421 gttttcgctg ccggtatttc cagaaaagta ctggaaagat cacaaactta gcgacccgct
    42481 cgccacgcct ccgcttgcca gtggtccgta ccgcattacg tcctggaaaa tggggcaaaa
    42541 tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgtggacg
    42601 ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc
    42661 gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac gccaaaaact gggccacgcg
    42721 ctataccggt aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc
    42781 agcccaggat acgcgctggc tggcgtttaa tatccaacgt ccggtattca gcgatcgccg
    42841 ggtgcgggaa gcgatcactc tcgcctttga ctttgaatgg atgaacaaag cgttgtttta
    42901 caatgcctgg agtcgcacga acagttattt tcagaatacc gaatatgcgg ccagaaatta
    42961 ccccgacgcc gcggaactgg tgcttctggc accaatgaaa aaagatctac cgccagaagt
    43021 cttcacgcaa atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct
    43081 gttaaaagcc gacaaacttc tcaacgaagc aggctgggtg ctgaagggtc agcaacgcgt
    43141 taatgtcaca acgggtcagc cactcagctt tgaattattg cttcccgcaa gcagcaatag
    43201 tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg
    43261 taaggtggat aactcgcaaa tcaccaaccg catgcgcagt cgcgactatg acatgatgcc
    43321 gcgcgtatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga
    43381 atatatcaat tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat
    43441 caaccaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact
    43501 ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg
    43561 tctcgcctgg tgggataaat tctcccaacc cgctgtacgc cctgtttaca gcctgggtat
    43621 cgatacctgg tggtatgacg ttaataaagc gacgaaactg ccgtcagcca gacaacaggg
    43681 agagtagatg ggcgcttatc tgattcgccg tctgttgctg gtgatcccaa cgttatgggc
    43741 gattatcact atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc
    43801 catcgccgcc attgagtttg gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt
    43861 tcgcgccagc catgcgcaaa cgggcgtcgg caatatcagc gacagtaatt accgtggcgg
    43921 acgcggatta gatccagaag tgatcgccga gatcactcat cgctacggtt tcgataagcc
    43981 gatccacgaa cgttacttca aaatgctctg ggactacatc cgttttgatt ttggcgacag
    44041 cctttttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg tctccatcac
    44101 cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa
    44161 agctgtttat aatgggagcc gttttgacgt ctggagtagt gcatttatca tcatcggcta
    44221 cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta
    44281 tttcgacctg ttccctctgc gcggcctggt ttccgctaac tttgattcgc tgccgtggta
    44341 tcagaaaatc accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg
    44401 cggctttgcg gctctgacca tgctgacaaa aaactcattc cttgatgaag tgcgtaagca
    44461 atacgtggtg acggcacgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt
    44521 gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt
    44581 ttttaccggc tcgctgctga ttgaggtgat gttttcactc aatggcctcg gcttactggg
    44641 ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac
    44701 cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg tcgatccgcg
    44761 tatagatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg
    44821 cgttttcgcc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt cttgtttggt
    44881 ttgagtttgt gttctgaact tatcgccaac gataaaccgt tgctggtgcg ttatgacggc
    44941 agttggtatt tcccgttgtt gaaaaactac agcgaaagcg attttggcgg cccgctggca
    45001 agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta
    45061 ctgtgggctc cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc
    45121 ccttctccac cctcccggca aaactggctg ggaacggatg ccaatggcgg cgatgtactg
    45181 gcacgcattc tctatggcac gcggatctca gttctgtttg gcctgatgct gaccctctgc
    45241 tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggtgg taaagtcgat
    45301 ctctgggggc aacgttttat tgaagtatgg tcggggatgc cgacgctgtt tttgattatt
    45361 ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac tgtcttgttt
    45421 agctggatga gtctggtcgg cgtggtgcgg gcggagtttt tacgtacccg taatttcgac
    45481 tacatccgcg cggcgcaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg
    45541 ttgccaaatg ccatggtcgc taccctcact tttttaccgt ttattttatg tagttcgata
    45601 accaccctga cctcactcga tttcctcggc ttcggtctgc cgctcggttc accgtcactc
    45661 ggtgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc
    45721 ttcttatcgg tggcgatatt gttgtctttg ctgatcttta ttggtgaagc cgtccgcgac
    45781 gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt
    45841 gtcagtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg tttcactaca
    45901 gattgcggct ggcgaaacgc tggcgctggt aggtgagtct ggttcaggta agagcgttac
    45961 cgcgctgtca attttacgcc tgctcccttg cccgccggtt gaatatctct ccggcgatat
    46021 tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg
    46081 taataagatc gccatgattt ttcaggagcc gatggtgtcg ttaaatccat tacataccct
    46141 ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg
    46201 tggcgaaatt cttaactgcc ttgatcgcgt cggtatccgc caggcggcaa aacggctgac
    46261 agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct
    46321 gttaacgcga ccggaattat taattgccga tgaaccaacc accgcgctgg acgtctctgt
    46381 ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata tgggcatgct
    46441 gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca
    46501 aaacggtcgc tgtgtcgagc aaaataacgc tgctacgcta tttgcctcac ccactcatcc
    46561 ttacacacaa aagctactca acagtgaacc atctggcgac ccggtgccat tgccagaacc
    46621 tgcctcaacg ttgctagatg ttgaacagct tcaggttgcc ttccccatcc gcaaagggat
    46681 tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgggc
    46741 gggtgaaaca ctgggtttag tgggcgagtc cggttccggg aaaagcacga cgggactggc
    46801 gctgctgcga ctgattaatt ctcatggcag catcgtcttt gacggtcagc cactgcaaaa
    46861 tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc
    46921 aaactcctca ctcaacccac gactcaacgt tttgcaaatt attgaggaag gcttacgggt
    46981 tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga
    47041 agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg
    47101 gcaacgtatt gcgattgcca gggcgttaat tcttaagccc tcgctgatca ttcttgatga
    47161 accaacatca tcactcgaca aaacggtgca ggcgcaaata ttgacgctat tgaaatcatt
    47221 gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc
    47281 gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc
    47341 gcgcgtgttt gccgcaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg
    47401 cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc
    47461 ggcatgttgc agcaaattcg tcctggcgat ttacaaacag gcacggctca ccttcacact
    47521 ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg
    47581 accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc
    47641 acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat
    47701 agttctgcgg cagtcgatgg acgattgaga gttgtaatgg agtcacttat tttccccgga
    47761 agcacattta tttcacagtt tatttacatc aactttaaca catcatcaac aatccgtctt
    47821 tatacagatc gtaaagattg atgcctcgcc agcgtggcct ctatggctct gatttaaata
    47881 atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc gatctaactc
    47941 aaccttttta actacaatga tgtgactttt tacataaatt gattttacat aaaatataca
    48001 tacatcgggg gaatgataga tttgtggttg acatgtatca acaaaggaag ccttttagct
    48061 tcctcgttgt gcaatagatc accgtttttt cggccgactg gcgtacaaac agaagagaat
    48121 ggaactggtt gcgcagaatg caattgacca aatcatcggc catgcagagt taaaggtcgc
    48181 aagagaaagt aatgcgccaa caattgcccc tatgccgaaa cggaaggttc ctgccagcga
    48241 agatgccgtt cccgccatat ggggaaactc atcaagaatg accgccatcg cattggatga
    48301 caccatcgac acgcagccca caaacgccgc aacgccaacc accagcgacc agaatcccag
    48361 ccccagcagc gcgctaatga ccatccacgc tgccataata aactgtatcc acaaccccga
    48421 gcggaacata tttaacgcgc caatacggcg gacgaagcgg ctgttaaaga tggtcatcac
    48481 gaacagaaaa agaatgttta gcgcaaagta ataaccaaag ttttccggcg cgacgtggtt
    48541 gatttcaatg tagacaaacg gtccggcgct taagaatgag aacatcccgg caaagctgaa
    48601 accgctggcc agcatataac tcaggacacg cttatgacgg aacagcgccg caaagttacc
    48661 aatagtggta cgaatgtgaa aaggctggcg acgctccggt ggtaaggttt ctttgatcag
    48721 aaagaagatc attgccgaag ccagaatcgc cgccacggcg aggatccaga agatgtaatg
    48781 ccagctcagc cacaccagca cccagccgcc aactatcggt gccatcagcg gtgcaatggt
    48841 cgtcaccagc atgacaaacg acatcatccg tgagaactct tctttcgggt agatatcacg
    48901 catcaaagcg ttgataacca cgctggccgc agccgcagcc agcccgtgga agaaacgcat
    48961 cacaatcagc tgatcgatgg tttgcgccaa cgcacacgcc accgcggcgg cggcaaacac
    49021 cagcgtaccg ccaagaacca ccggcttacg cccgaagctg tctgccatcg gcccgtagat
    49081 taactgcccc aacgcaaagc ccagaatata agtactcagg gtcatctgcg tactgcccgc
    49141 cggtacacca aactgcgctg aaattaccgg tagcgcaggc agatacatat caatcgacag
    49201 cggcatcaac atggtcagca ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg
    49261 ggtggtcaca acgggctcct gaaagtcatt gaaaagttag acgacgctgg caatttcttc
    49321 ttcagttaac ggacgatatt caccgggggc taaatcagca tccagcgtaa tgccgccaat
    49381 acgttcacga tgcagctcaa ccacatggtt acccacggcg gcgaacatgc gtttcacctg
    49441 atgataacgc ccttcgctga tggtcagacg aacctgcgtt ggggtaatca cttccagcac
    49501 cgccggctta gtgagatctt tttcgttatg cagctgcacg cctttagcaa attgctctgc
    49561 cgtatcgtca gctacgggtg attccagcgt caccagatag gtcttctcgc aatgatggcg
    49621 cggggaagta atgcggtgcg accactgacc atcatctgtc attagtacca gaccggtggt
    49681 atcgatatcc agacgccctg cagcgtgcag tttccacgct accggttcat caagaaaata
    49741 gagcaccgtt gggtgatccg ggtcgtccgt ggagcaaaca tagccctgag gcttattgag
    49801 catgaagtaa cgtggaccgt gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg
    49861 ttcagggagc agtttgaacg ctgcattacg gacgatttcg ccatcgacgg tgacacgatt
    49921 gccgcggatt tcacgcccgg caatagcacg gctaacgccg agttgctgtg cgataaattt
    49981 atcaagtcgc atgtgtgtga ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa
    50041 atctgaactg ttgtctgccc agtatagcgg tctaataact tccctcaagg gaaaaagatc
    50101 cgtggcatac tattagcaga ataatctacc tacgcgagac catgattttt acactccgcc
    50161 catatcagca agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg
    50221 ccgtaatcgt gctgcccacc ggtgcaggta aaagcctggt gatagcggaa ctggcgcggc
    50281 tggcgcgtgg tcgcgtgctg gtgttggcac acgttaaaga actggtggca caaaaccatg
    50341 aaaaatatca ggcgctgggg ctggaagccg atatttttgc cgccgggcta aagcgcaaag
    50401 agagccacgg taaagtggta tttggcagcg tgcagtcggt cgcccgtaat cttgatgcct
    50461 ttcagggtga attttcgctg ttgattgtcg atgaatgcca ccgtattggt gacgatgaag
    50521 agagccagta tcagcaaatc ctcactcacc tgaccaaagt gaatccccac ttacgcctgc
    50581 tggggctgac tgccacgcct tttcgactgg gcaaaggctg gatctaccag ttccattatc
    50641 acggcatggt acgcggcgat gagaaagccc ttttccgtga ctgcatttat gagctgccgc
    50701 tgcgttatat gattaaacac ggctatctga cgccgccaga acggctggat atgccagtag
    50761 tgcaatacga tttcagccgc ttgcaggcac agagtaacgg actgttcagc gaagccgatc
    50821 tcaaccgcga gctgaaaaaa cagcaacgca ttacgccaca catcatcagc caaatcatgg
    50881 agtttgctgc aacgcgcaaa ggggtgatga ttttcgccgc gacggtcgaa cacgcaaaag
    50941 agattgtggg attactgcct gccgaagatg cagcactgat tactggcgac acacccggcg
    51001 ctgagcgcga tgtattaatt gatgatttta aagcccagcg ttttcgctat ctggtcaacg
    51061 tcgcggtact gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc
    51121 ctaccgaatc ggtgagtctt taccaacaaa ttgtcgggcg cggcctgcgc ctggcccccg
    51181 gcaagactga ttgcttaatt cttgattatg cgggtaaccc tcacgatctc tacgcgccgg
    51241 aagttggtac accgaaaggc aaaagtgaca acgttccggt acaggttttc tgccctgcct
    51301 gcggttttgc caacaccttt tgggggaaaa cgactgccga cgggacattg attgaacact
    51361 ttggtcgtcg ctgtcaggga tggtttgaag atgacgacgg acatcgcgaa cagtgtgact
    51421 tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt gccgcccgcc
    51481 gttgccgcga gtgtgatacc attctggttg acccggatga tatgttaaaa gcggcgctac
    51541 gactgaaaga cgcgctggta ttacgctgta gcggcatgtc tttgcagcat gggcacgacg
    51601 aaaaaggcga atggttgaaa atcacctatt acgatgaaga cggcgcggat gtgagtgagc
    51661 gtttccgtct gcaaacgccc gcccagcgaa ctgccttcga gcagcttttt atccgcccgc
    51721 atacgcgcac accgggcatc ccgctgcgct ggatcaccgc cgccgatgtc ctcgcccagc
    51781 aagccttatt gcgacacccg gattttgtcg tcgcccgcat gaaaggccag tactggcaag
    51841 tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt
    51901 aatggtagtt ttgattgatg tataaggcga ttgagtatag aatctcgccc gcttttgcat
    51961 acgcaaagca gatcacttac ctgttgctgg gtcgcctgta gcaggattaa tttaagagag
    52021 aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc
    52081 cgcctgcgtg ccgctaacaa gttcccggca atcatctacg gtggcaaaga agcgccgctg
    52141 gctgtcgaac tggatcacga caaagtcatg aacatgcaag ttaaagctga attctacagc
    52201 gaagttctga ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag
    52261 cgtcacccgt acaaaccgaa gctgctgcac atcgacttcg ttcgcgctta attgctgaat
    52321 aagttgtaaa aaaccccgct ccggcggggt tttttgtctc tgcaaattat gcctgattac
    52381 ggtatcgcta ttttttgcag gccagataag gcgttcacgc cgcatctggc attcagaaca
    52441 caaaaccgat taattgccgc cagacgtccg gcgctgcaat tgatcgcgca agttcggcgg
    52501 cgtgccttta atggtcaggg tatcggttgc tggatcccag aagatgcgtt cacctaacag
    52561 catcgcatca aagttaatag tcaagccacc gccgctaccg gcaaacttcg tcaactgacg
    52621 cagtgtgcta cggtctgccg ggaagctctc ttccagttcg tagccttttt cagccgcaaa
    52681 ctccgtgaag ctcacttcgc tgacgcctgc cagctctttc gacagcgatt ccagttcaat
    52741 ctcttcacct gcctgcagct gctcattaca gtagctataa acctgctggc gcacgttctg
    52801 tcgctctgct ttatccagtt gtgcttcggc agtgaaatca tcgaccgctt gcaacagccc
    52861 acggttttgc gctttcgcgt ttaacccttc gctggcaccg aggaaatcca taaagaaatc
    52921 cgccactttg cgccctactc gccctttcag gaaagtgaga tagcgggtcg actctggatt
    52981 ggtttcccac tcggttaaat cgatacgcgc aacaatatcc gcatgattga tatcaagata
    53041 atgggttggg ttgatgtcga gattttcgtt aacacgcata ctgctcaggt tgctcagaac
    53101 tgccaccagc agatactcca ccgccagata gcgatagtgg caaaacagca cgaatccacc
    53161 gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa
    53221 tgccagaaag tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca gttcactctc
    53281 ttcgctaaac aagccgtaag ctttattttt ggcgctatag acccgatgca gttctgccac
    53341 catctcaacg acggtttccg tcggttccag caatgaatcg cgcaacacca gctcaaggtt
    53401 ctgctcatca cgcttgataa gctggtgcag agcaatctgg ttgatatcca gactcatgat
    53461 aaactctcct ttaagaccgg gcggtattca accaccgccg gttaagcgac gcaataaaag
    53521 atggcggtca ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga
    53581 aaaaaaaccg ttgctacggt aatatgttgc cctttcatca actaactgat ttcgatttat
    53641 gccacaaatt tcccgctaca gtgatgaaca ggttgaacaa ttgctcgcag agctgctcaa
    53701 cgtactggaa aaacataagg ctccgaccga cctttccctg atggtgttag gcaacatggt
    53761 cactaacctt atcaacacca gcattgcccc ggcccaacgc caggcaattg ccaactcttt
    53821 tgcccgcgcc ttacagtcct ctatcaacga agacaaagcg cactaaggga aacagataac
    53881 aggttatggt aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg
    53941 ggcactggtt tgcactgttc aatattctgc tttcgctcgt cattggcagc cgttacctgt
    54001 ttatcgccga ctggccgaca acgcttgctg gtcgcattta ttcctacgta agcattatcg
    54061 gtcatttcag cttcctggtg ttcgccacct acttgctgat cctcttcccg ctaaccttta
    54121 tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat tctggcaacg gcgggaatga
    54181 cgctattact gatcgacagc gaagtcttta ctcgtttcca tctccatctt aatcccatcg
    54241 tctggcagct ggttatcaac ccagacgaaa atgagatggc gcgcgactgg cagctaatgt
    54301 tcatcagtgt gccggttatt ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa
    54361 agctgcgcag cctgacgcgt cgtcggcgct tcgcgcgccc gctggccgca ttcttattta
    54421 tcgcctttat cgcctcgcat gtggtttata tctgggccga tgccaacttc tatcgcccga
    54481 tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat gacggcacga cgttttcttg
    54541 agaagcatgg cctgcttgat gcgcaggagt atcaacgccg tcttattgag caaggcaatc
    54601 cagacgccgt ttccgttcag tacccgttaa gcgaactgcg ctatcgcgat atgggcaccg
    54661 ggcagaatgt gctgttgatt actgtcgatg gcctgaacta ctcacgcttc gagaagcaga
    54721 tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt cacgcgtcat atgagctccg
    54781 gcaacactac agacaacggc atctttggcc tgttttatgg catctcgcca agctatatgg
    54841 acggcatttt gtcgacccgt acgcctgcgg cgttaattac tgcgcttaat cagcaaggct
    54901 atcagctggg gttattctcg tcagatggct ttaccagccc gctgtatcgg caggcattgt
    54961 tgtcagattt ctcgatgccg agtgtacgca cccaatccga cgaacagacc gccacgcagt
    55021 ggatcaactg gctgggccgc tacgcacaag aagataaccg ctggttctcg tgggtctctt
    55081 tcaatggcac aaacattgac gacagcaatc agcaggcatt tgcacggaaa tatagccgag
    55141 cagtaggcaa tgtcgatgac cagatcaacc gcgtgctcaa tgcactgcgt gattctggca
    55201 aactggacaa tacggtggtg attatcactg ccgggcgggg tattccgctg agcgaagagg
    55261 aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc attagtgatt cactggccag
    55321 gcacgccggc gcagcgtatt aatgcgctga ctgatcatac cgatctgatg acgacgctga
    55381 tgcaacgcct gctacatgtc agcacacctg ccagcgaata ttcgcaaggt caggatttgt
    55441 tcaaccctca acgccgccat tactgggtta ccgccgcgga taacgatacg ctggcaatta
    55501 ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa ataccgcact tacaacttac
    55561 gtggtgaaag agtgaaagat gaaaaaccac agttaagttt gttattgcaa gtactgacag
    55621 acgagaagcg ttttatcgct aactgattaa ttataaatca gttagcaaaa tatcttactt
    55681 gcaatcggtg tggaaaacgg tagtattaac agccacgagt cggcacgtag cgcagcctgg
    55741 tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa
    55801 atcccaagaa aaccaaccta ttgcggttgg tttttttata tctgcattta attcgataaa
    55861 cagacagcga cacatcacga cctgtttatt ttctgttatc agaacgtcca gactacaccc
    55921 gcctgagttg tgctgctttc ttcacgggag aaacgactat cgataccgag cgtcaggccc
    55981 gtcaatgttt gctcaaagcc agctcccgca tcagtgttca cgttgttgca gctgttaatt
    56041 gccgaactcc acatcgccgt atcgtaatta acgcctttta cgctaccaag acgcccacgc
    56101 acggtgtcca gttctgcatc aaataccagc ggttgtgctg ccgccatatt cagcacatcc
    56161 gtggttgaag ggggcatttg tacgcgattc tctgcgagac tccagctatg gttgccatta
    56221 tccagcaagg tatattcata cgtactgata tcaacaacgc ttccggcatt gcccaacgta
    56281 aatgccgcat caccgcgccc tcctttaatc gtgggatcag tcgccgcgcc ataaacctgt
    56341 agcactccat tattgatcgt agtctggttt accgcgccac ccatttgaac aatttgataa
    56401 ccatcattta atactgagta ttctgcggtg ccatttattt caatgtactg atatcccccg
    56461 ttaatttcag tattactact tacacctaat tcttatatat gctgttctcc gccaggattg
    56521 attttcgtat tattggcggt ccaataaacc agctggatat ctttttccag gacaacacca
    56581 tcaactgtct caccatactc aacagttaat gccaatgcta tgggtgaata taacgctgta
    56641 gccaccagaa cagatattgc ggaacgacaa agagaaacag aaccagattg gtgcattaag
    56701 ctttcatcct atgaaattaa ttgctgttaa aagcattggg gacagaaaat acccatagct
    56761 ccatacccgg agtcagtttt taaaaactgt ttaaagaaat gcacaagtat tgtgattgat
    56821 tttttagttg tttttcctga tgagaagctg atgcaaaatt ccgtctttat aatgaagatg
    56881 atgccaaagc gaacgacaaa gttgtagttt tcactacatg tccatacata aaatggggta
    56941 acattcacgc gcctggtagc gttaccaacg ctacgctcaa acataatgat tctaataaaa
    57001 cctcaggaga ctactatgcc tgaagcaacg ccttttcagg tgatgattgt ggatgatcat
    57061 ccacttatgc gacgcggtgt tcgtcagtta ctggagcttg attctggctt tgaagtggtc
    57121 gccgaagcgg gcgacggcgc aagcgcgatc gatctggcga atagactgga tatcgacgtg
    57181 atcttgctgg atctcaatat gaaaggcatg agtggcctgg ataccctcaa tgccttgcgc
    57241 agggatggcg ttaccgcgca aattattatc ctgaccgtat cagatgcctc cagcgatgtc
    57301 tttgcactga tagacgcagg cgcagacggt tatctgttga aagacagcga cccggaagtg
    57361 ttactggaag caattcgtgc cggagcgaaa ggcagcaaag tctttagcga acgcgtcaat
    57421 cagtacttac gtgaacgtga aatgtttggc gcggaagaag accccttcag cgtgctgaca
    57481 gaacgcgagc tggatgttct gcacgagctg gcacaggggc tgtcaaataa acagattgcc
    57541 tcggtgttga atatttccga gcagacagta aaagtccata ttcgcaatct gctgcgtaaa
    57601 ctcaacgtcc gctcacgcgt ggcggccacc attctgttcc tgcaacaacg cggggcacaa
    57661 taaaaatagc ccgatggcat caccatcggg ccacttttta tttactctcc tgcggcgaca
    57721 aatgttgcat cgcctgcgcg atactacgtt cgatcaccgc acggcgagta tcgttggcag
    57781 gtaagagttt caacatcatc tcccacgcgg caacggcttc gccaaatcgt tgctgctcaa
    57841 aggcattaaa tgcatacatg cttaacacac ggatattgct atggtccgtt ctcaccaact
    57901 ggcgcagcag ttcgccgccg aggcggttgt cgttgggatc agatgaacgt gtcaacgctt
    57961 cagcgtatcc cagtgcagca tcgctgtttt tcggatcgag gcgatacgca gtggcgtatg
    58021 catcggtggc gatactggcg ttacccagcg ccatgccaac gcggcccaac ataatccagc
    58081 cttctatatc tcccggattt ttttgcagtt gagtacgcat ccccagcgca aggcgcgaca
    58141 tctcttcttc gttgagcgga tcggctttcg gatccagcgc cctgtccagt aacgccggag
    58201 cctgtgccgt ggcctgctgc cagattttca cctgctgata attgccagtc tggtagtagc
    58261 tgacgccagc cacaattaac gccaccacaa tacccggcag ataaacaata tatccggcac
    58321 gcttaccttc cggcacgctt tgttctggaa acgcttccgg caccacgcgt acccgacgcc
    58381 gcgaacgggc gtatatgacc caaccgccaa tgccaatagc cactactggc agcacccaca
    58441 gcagcacggt cagcggcgtt aacggcggat cgtaagtgac gaagttgccg taacgcgcca
    58501 ccatataatc gacaatctct ttcttacttt tgccttcctg catcagttca tacactttct
    58561 ggcgcaggtc ggtggcaatc atcgagttgg aatcggcaat gctgttgttc tggcatttcg
    58621 ggcagcgcag ttcttcagtg agctgacgga actgttgttc ctgtgcttca tctttaaact
    58681 gcaacacgtc gatagtcgcc agcgctgagc cggagatcat cagcatcagc acgcccaata
    58741 aaaacctcat tgtgcggcct ccttactgta tttctcccac agtggcttga tctcttcttc
    58801 ccagacgcga ggattgagat cgcccgcatg gcgatagcga atgatgccgt tgccgtcaat
    58861 aaggaacgtt tctggcgcgc catagacacc gagatccagc cctaacatgc cgtcgccatc
    58921 aaacaggctt agcgcgtaag gattgcccag ctctttcagc cagctgattg ccttctggcg
    58981 atcgtcttta tagttcatgc cgaccacgcg gatgccctgc gcagaaagct gattcagata
    59041 ttgatgttcc gcacggcagg tcggacacca ggtcgcccag acgttaagca gcaccggttt
    59101 gccctgagtc agcacatccg cctgataaaa ctgccccgga ttgtccagtg attcgaggcg
    59161 aaacttcggc actggcttgc caatgagcgc cgattccaga ttggtcggat catccccttc
    59221 ggcattacgc gccagctgcc acagtagcgc cgtggcaatc gccaggaaga taatcaacgg
    59281 aattaacaat actttgcgct tcatacagcc tccggcgcag ttttctgcgg gttcacgcgc
    59341 ttacgatagc gaggatcaaa
//
LOCUS       DAZFRE010000019        56361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFRE010000019 DAZFRE010000000
VERSION     DAZFRE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.019"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..436
                     /locus_tag="ACHKUI_001398"
     CDS             <1..436
                     /locus_tag="ACHKUI_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6597634.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQF
                     AVQSGPLLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            519..1421
                     /locus_tag="ACHKUI_001399"
     CDS             519..1421
                     /locus_tag="ACHKUI_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO6597635.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTERKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="ACHKUI_001400"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="ACHKUI_001400"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6597636.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="ACHKUI_001401"
     CDS             complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="ACHKUI_001401"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6597637.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="ACHKUI_001402"
     CDS             complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="ACHKUI_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6597638.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5321..6028)
                     /gene="yigA"
                     /locus_tag="ACHKUI_001403"
     CDS             complement(5321..6028)
                     /gene="yigA"
                     /locus_tag="ACHKUI_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO6597639.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="ACHKUI_001404"
     CDS             complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="ACHKUI_001404"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6597640.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPDLIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6886..7089)
                     /gene="yifL"
                     /locus_tag="ACHKUI_001405"
     CDS             complement(6886..7089)
                     /gene="yifL"
                     /locus_tag="ACHKUI_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6597641.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7278..8747
                     /locus_tag="ACHKUI_001406"
     CDS             7278..8747
                     /locus_tag="ACHKUI_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6597642.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLLAPFGPWDTLARTQTKIMINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            8744..10072
                     /locus_tag="ACHKUI_001407"
     CDS             8744..10072
                     /locus_tag="ACHKUI_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6597643.1"
                     /translation="MNGQIITVALILLGHIFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGAHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSQWIDLQGIEPEQFHLITDNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDSPDLVIDHSFNRGKQHAYFIHQLRSRKSVQIIPTNNGPLTKNDRFAYDDRNVYTWT
                     DTEVRITPSPCPAKTRVREENVHELHNRDIIIPLTDESCQNAADEQTLKP"
     gene            10120..10440
                     /gene="cyaY"
                     /locus_tag="ACHKUI_001408"
     CDS             10120..10440
                     /gene="cyaY"
                     /locus_tag="ACHKUI_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6597644.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(10480..13026)
                     /gene="cyaA"
                     /locus_tag="ACHKUI_001409"
     CDS             complement(10480..13026)
                     /gene="cyaA"
                     /locus_tag="ACHKUI_001409"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6597645.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            13413..14354
                     /gene="hemC"
                     /locus_tag="ACHKUI_001410"
     CDS             13413..14354
                     /gene="hemC"
                     /locus_tag="ACHKUI_001410"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6597646.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14351..15091
                     /gene="hemD"
                     /locus_tag="ACHKUI_001411"
     CDS             14351..15091
                     /gene="hemD"
                     /locus_tag="ACHKUI_001411"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6597647.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIRDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            15113..16324
                     /gene="hemX"
                     /locus_tag="ACHKUI_001412"
     CDS             15113..16324
                     /gene="hemX"
                     /locus_tag="ACHKUI_001412"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6597648.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     ADAPAAAPQGE"
     gene            16327..17523
                     /gene="hemY"
                     /locus_tag="ACHKUI_001413"
     CDS             16327..17523
                     /gene="hemY"
                     /locus_tag="ACHKUI_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6597649.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            18202..19857
                     /gene="aslA"
                     /locus_tag="ACHKUI_001414"
                     /pseudo
     CDS             18202..19857
                     /gene="aslA"
                     /locus_tag="ACHKUI_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
     gene            complement(19948..21183)
                     /gene="aslB"
                     /locus_tag="ACHKUI_001415"
     CDS             complement(19948..21183)
                     /gene="aslB"
                     /locus_tag="ACHKUI_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6597650.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALSLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(21330..21406)
                     /locus_tag="ACHKUI_001416"
     tRNA            complement(21330..21406)
                     /locus_tag="ACHKUI_001416"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21370..21372),aa:Pro,seq:tgg)
     gene            complement(21449..21535)
                     /locus_tag="ACHKUI_001417"
     tRNA            complement(21449..21535)
                     /locus_tag="ACHKUI_001417"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21499..21501),aa:Leu,seq:cag)
     gene            complement(21556..21631)
                     /locus_tag="ACHKUI_001418"
     tRNA            complement(21556..21631)
                     /locus_tag="ACHKUI_001418"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21596..21598),aa:His,seq:gtg)
     gene            complement(21690..21766)
                     /locus_tag="ACHKUI_001419"
     tRNA            complement(21690..21766)
                     /locus_tag="ACHKUI_001419"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21730..21732),aa:Arg,seq:ccg)
     gene            complement(21869..23254)
                     /gene="yifK"
                     /locus_tag="ACHKUI_001420"
     CDS             complement(21869..23254)
                     /gene="yifK"
                     /locus_tag="ACHKUI_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6597651.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(23445..24185)
                     /gene="wecG"
                     /locus_tag="ACHKUI_001421"
     CDS             complement(23445..24185)
                     /gene="wecG"
                     /locus_tag="ACHKUI_001421"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6597652.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(24188..25540)
                     /gene="wzyE"
                     /locus_tag="ACHKUI_001422"
     CDS             complement(24188..25540)
                     /gene="wzyE"
                     /locus_tag="ACHKUI_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6597653.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(25537..26616)
                     /gene="rffT"
                     /locus_tag="ACHKUI_001423"
                     /gene_synonym="wecF"
     CDS             complement(25537..26616)
                     /gene="rffT"
                     /locus_tag="ACHKUI_001423"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6597654.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSGHAREFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(26613..27863)
                     /gene="wzxE"
                     /locus_tag="ACHKUI_001424"
     CDS             complement(26613..27863)
                     /gene="wzxE"
                     /locus_tag="ACHKUI_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6597655.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(27865..28995)
                     /gene="rffA"
                     /locus_tag="ACHKUI_001425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(27865..28995)
                     /gene="rffA"
                     /locus_tag="ACHKUI_001425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6597656.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAEAGR
                     IELPSIPDDCVQNAHMFYIKLRDIDDRSALISFLKEAEIMAVFHYIPLHASPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(29000..29674)
                     /gene="rffC"
                     /locus_tag="ACHKUI_001426"
                     /gene_synonym="wecD"
     CDS             complement(29000..29674)
                     /gene="rffC"
                     /locus_tag="ACHKUI_001426"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6597657.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKITLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(29652..30533)
                     /gene="rfbA"
                     /locus_tag="ACHKUI_001427"
     CDS             complement(29652..30533)
                     /gene="rfbA"
                     /locus_tag="ACHKUI_001427"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6597658.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(30552..31619)
                     /gene="rffG"
                     /locus_tag="ACHKUI_001428"
     CDS             complement(30552..31619)
                     /gene="rffG"
                     /locus_tag="ACHKUI_001428"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6597659.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWCKQVQDGSYQGERLGLKG"
     gene            complement(31619..32881)
                     /gene="wecC"
                     /locus_tag="ACHKUI_001429"
     CDS             complement(31619..32881)
                     /gene="wecC"
                     /locus_tag="ACHKUI_001429"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6597660.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRVSELKIACFGLAFKPNI
                     DDLRESPAMEIAELIARWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(32878..34008)
                     /gene="wecB"
                     /locus_tag="ACHKUI_001430"
     CDS             complement(32878..34008)
                     /gene="wecB"
                     /locus_tag="ACHKUI_001430"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6597661.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(34064..35110)
                     /gene="wzzE"
                     /locus_tag="ACHKUI_001431"
     CDS             complement(34064..35110)
                     /gene="wzzE"
                     /locus_tag="ACHKUI_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6597662.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(35122..36225)
                     /gene="wecA"
                     /locus_tag="ACHKUI_001432"
     CDS             complement(35122..36225)
                     /gene="wecA"
                     /locus_tag="ACHKUI_001432"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6597663.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGAVDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILLFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(36465..37724)
                     /gene="rho"
                     /locus_tag="ACHKUI_001433"
     CDS             complement(36465..37724)
                     /gene="rho"
                     /locus_tag="ACHKUI_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6597664.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(37809..37910)
                     /gene="rhoL"
                     /locus_tag="ACHKUI_001434"
     CDS             complement(37809..37910)
                     /gene="rhoL"
                     /locus_tag="ACHKUI_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6597665.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(38051..38380)
                     /gene="trxA"
                     /locus_tag="ACHKUI_001435"
     CDS             complement(38051..38380)
                     /gene="trxA"
                     /locus_tag="ACHKUI_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6597666.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            38511..39776
                     /gene="rhlB"
                     /locus_tag="ACHKUI_001436"
     CDS             38511..39776
                     /gene="rhlB"
                     /locus_tag="ACHKUI_001436"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6597667.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            39912..41396
                     /gene="gppA"
                     /locus_tag="ACHKUI_001437"
     CDS             39912..41396
                     /gene="gppA"
                     /locus_tag="ACHKUI_001437"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6597668.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(41443..43464)
                     /gene="rep"
                     /locus_tag="ACHKUI_001438"
     CDS             complement(41443..43464)
                     /gene="rep"
                     /locus_tag="ACHKUI_001438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6597669.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMLKGQSHLANLKAMMAAKRGK"
     gene            43551..43832
                     /gene="ppiC"
                     /locus_tag="ACHKUI_001439"
     CDS             43551..43832
                     /gene="ppiC"
                     /locus_tag="ACHKUI_001439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6597670.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(43878..45353)
                     /gene="ilvC"
                     /locus_tag="ACHKUI_001440"
     CDS             complement(43878..45353)
                     /gene="ilvC"
                     /locus_tag="ACHKUI_001440"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6597671.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            45503..46393
                     /gene="ilvY"
                     /locus_tag="ACHKUI_001441"
     CDS             45503..46393
                     /gene="ilvY"
                     /locus_tag="ACHKUI_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6597672.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGSVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(46390..47934)
                     /gene="ilvA"
                     /locus_tag="ACHKUI_001442"
     CDS             complement(46390..47934)
                     /gene="ilvA"
                     /locus_tag="ACHKUI_001442"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6597673.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQLQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(47937..49787)
                     /gene="ilvD"
                     /locus_tag="ACHKUI_001443"
     CDS             complement(47937..49787)
                     /gene="ilvD"
                     /locus_tag="ACHKUI_001443"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6597674.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSAQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(49852..50781)
                     /gene="ilvE"
                     /locus_tag="ACHKUI_001444"
     CDS             complement(49852..50781)
                     /gene="ilvE"
                     /locus_tag="ACHKUI_001444"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6597675.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(50801..51064)
                     /gene="ilvM"
                     /locus_tag="ACHKUI_001445"
     CDS             complement(50801..51064)
                     /gene="ilvM"
                     /locus_tag="ACHKUI_001445"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6597676.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(51061..52707)
                     /gene="ilvG"
                     /locus_tag="ACHKUI_001446"
     CDS             complement(51061..52707)
                     /gene="ilvG"
                     /locus_tag="ACHKUI_001446"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6597677.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(52710..52760)
                     /gene="ilvX"
                     /locus_tag="ACHKUI_001447"
     CDS             complement(52710..52760)
                     /gene="ilvX"
                     /locus_tag="ACHKUI_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6597678.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(52847..52945)
                     /gene="ilvL"
                     /locus_tag="ACHKUI_001448"
     CDS             complement(52847..52945)
                     /gene="ilvL"
                     /locus_tag="ACHKUI_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6597679.1"
                     /translation="MTALLRVISLVVISMVVIIIPPCGAALGRGKA"
     gene            53298..54818
                     /gene="yifB"
                     /locus_tag="ACHKUI_001449"
     CDS             53298..54818
                     /gene="yifB"
                     /locus_tag="ACHKUI_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6597680.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRHRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(54843..55181)
                     /gene="maoP"
                     /locus_tag="ACHKUI_001450"
     CDS             complement(54843..55181)
                     /gene="maoP"
                     /locus_tag="ACHKUI_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6597681.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            55300..56139
                     /gene="hdfR"
                     /locus_tag="ACHKUI_001451"
     CDS             55300..56139
                     /gene="hdfR"
                     /locus_tag="ACHKUI_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6597682.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(56235..56310)
                     /locus_tag="ACHKUI_001452"
     tRNA            complement(56235..56310)
                     /locus_tag="ACHKUI_001452"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56275..56277),aa:Trp,seq:cca)
BASE COUNT        13834 a        15250 c        14313 g        12964 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttattcgtc ctggcggagt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgccttta aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc attgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaacgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacaa tttcacatat
      361 gtatatgaaa ggcgaggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcagaaa ggttaaacat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctggaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacat
      541 tatttttatt aacctgtctg gggtcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaacggattt tagcctgaaa gatggcgccg tctcggcgat gaaaggggtc gctttcggcg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagactgcca gttactggcc gcaaggttcg gtgtttgtta
      781 gcgtggtcga tccaggtgtt ggtacagagc gtaaatcggt agtgctgaaa accaaaaacg
      841 gtcagtactt cgtttcacca gataacggta cgcttacgtt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgtct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgtctggct tccggggcga
     1021 tcacgttcga gcaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtgaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag ctttggcgat gtgccggaag gccagccgat ggtgtatctg aacagcttat
     1321 tgaatgtttc cgtggcgctg aatatggata atttcgcgca gaaacatcag gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcggaatgcg
     1441 gtgtggcgca ccgcattccg caacgtcaca ccgtctccaa ccgggcgtaa gccgccacca
     1501 gccatttaat accctggccc tggaatgcca cctgcaaacg gctatgttca ccgctgcctt
     1561 ccatattgac gatggtgcct tcaccaaact tagcgtggcg tacgcgctgg ccgagcttgt
     1621 agccgctgtc gttctcgacc atcggcgtac ccatccgttg atggctgacc ggacggctta
     1681 ccgtagcgcg caggcgcacc tcttccacac actcttccgg cagctcgcca ataaagcgcg
     1741 acgggcgatg gtaaacctct ttgccataca gacggcgggt ttccgcgtag gtcagcgtca
     1801 gtttctgcat cgcgcgggtt acgccaacgt aggccagacg gcgttcttct tccagacgcc
     1861 cgccttcatc cagcgacatc tggcttggga acatgccctc ttccatacca acgataaaca
     1921 cctgcgggaa ttccagacct ttcgccgagt gtagcgtcat caactgcacc gcgtcctgcc
     1981 aggtatccgc ctgcccctcg cccgcttcca gcgccgcatg ggagaggaat gcctgcagcg
     2041 gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc accagttcct
     2101 ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac atggtgcgca
     2161 ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg gcagtttcct
     2221 gcgctaaggc gtcgatcagc tccataaacc gctgtaaggc gctggcagca cgtccggcga
     2281 gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg cgatctcgcg
     2341 atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc gtattaacca
     2401 cacgctcaaa ggccgcgtcg tcgttgcggt tggcaatcag gcgcagatac gagagcgcat
     2461 ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacgatac ggcatactcg
     2521 cctgcaataa cgcctcttcc agcacacgcg actgggcgtt gctgcggtag agaatggcgc
     2581 actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc acaaaacgcg
     2641 cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc gcgccatcgg
     2701 tccacagttt tttacccaga cgcccgttat tgttttcaat cagggcgtta gcggcgctca
     2761 gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca ccggggaaat
     2821 cattgaggaa acgctgaata ttctccacct gcgctccgcg ccagccgtag attgactggt
     2881 cgtcatcgcc aacgatcatc actttgccag tatcgcctgc cagcagtcgg atccacgcat
     2941 actgaatgtt gttggtgtcc tggaattcat ccaccagaat attggtgaaa cgttcgcggt
     3001 aatgttgaag gatatgcggt ttgttgagcc ataattcgtg ggcacgaagc agcaattcag
     3061 caaaatcaac caggcccgcg cggtcacacg cttcctgata cgcctgatac accttctgcc
     3121 aggtctgctc caccggatta ccgtagctct gaatatgatg cggacgcagc ccctcatctt
     3181 tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg tcgaggttca
     3241 tcgccttgat taggcgtttg agcaggcgta gctggtcctc gctgtcgagg atctggaaat
     3301 cctgcggcag gttggcgtcc atatggtgcg cacgcagcag gcggtgcgcc agcccgtgga
     3361 aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata cgatgacgca
     3421 tctccgccgc cgctttgttg gtaaacgtca ccgccataat cgaatacggc gagcagtttt
     3481 ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca ctgcccgcgc
     3541 ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt ttatcattaa
     3601 ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag gttgggcgga
     3661 gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttgctg attatatcag
     3721 cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc tgtcccaggt
     3781 ttgcatcaga tcgccatttt ccggtctgat ccaacaggcc tgcattccgc tgcgaattgc
     3841 cccacccacg tcggtggtaa gatcgtcacc tacatgtaag atctcgccga tcggcacgtt
     3901 gagtttttcc gccgccagga agtacatatc gctgaacggt ttcgaacgcc cgtgcgggcc
     3961 agcgcgcagc acaaactcaa aataatcccc cagaccaaac agctccggct gggcgttacc
     4021 gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt cgtgagtttg
     4081 ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg cgtgtgcgcc
     4141 tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa tcgaacgaaa
     4201 acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg cctggcgcag
     4261 gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt gcacaaaggt
     4321 aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg tatcatccag
     4381 gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta tttcccccgt
     4441 ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc aagatgagta
     4501 tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc acgaagatcg
     4561 ccgctcgact ccagcatatg cgtagcgaac gagtgacgta atttatgcgg gtgaacgtga
     4621 ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt acgcgcggag
     4681 atacgtttgc ccagtttcga cagaaaaagc gcgtcgtctt cactaccaaa caggtcgcgc
     4741 aaatcaagcc agtgctcaat ccacgccacg gcgttgcgac caatcggcag gcggcgctct
     4801 ttgctgcctt ttcccatcac ccacacttcg ccagactcca ggtcgaggtg tttgatatcg
     4861 agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag cattgcccga
     4921 tcgcgcacgg cgaggggatc gttgatatca atatccagca gccgattcat atcatcgacg
     4981 tcgatgtttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt cgccgggtta
     5041 gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc agaaagccgt
     5101 aacgccagac ttgcagctcc cagcccttta cggcgactgc gtacagcaaa attacgcacc
     5161 atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa atggatgatc
     5221 gcctcaagct gtcgctggta gttaagcagg gttatcgggc taagctggcg ctccacgctc
     5281 agataacgta gatagcgttc tacatcggtg tgtaaatcgg tcatacgcgt tcaatccaac
     5341 gcgccagaag ctccggcaac atcagtgcaa tttcatgaag taactgcgtt ccctgccctt
     5401 gttgatagtg actggcatcg cgactggtaa acagcacaac acccaaatca gcatcgcttc
     5461 ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc accagaagct
     5521 ccggtccgtt aagcggcccc agatagtgct gttcctgccc cagacgctga atacgcagcg
     5581 gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca cctaagcgcc
     5641 agcgatccgg aaacagacgc agactcgcac ctgccagccc taaatcacgc gcccagcgat
     5701 ggaaacgcat cagcatatcg tcgagactac tggcggcggt gagactgcgc tgcaggtaga
     5761 gtagacgata aaacaggcct tcgttggcga tagcctgttc catcaacaac gccatgttct
     5821 cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc aacgaaacgg
     5881 tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcattg cggataaaaa
     5941 actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc gtgagtgttt
     6001 cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga catgtaccgc
     6061 cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa gacgaccgcc
     6121 agggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta ccgccaccgc
     6181 cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat aaacgcgtaa
     6241 acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct ccggaaaacg
     6301 ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga catcatcgac
     6361 ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg tctgctcggc
     6421 ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg cggaaggttc
     6481 gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg tcagaaccat
     6541 ccgcccgttg gcggtgctga cgcggatatc gcgcttattg gtcagccctt tcaggcgcac
     6601 aaaacgggca aagcagcgcg cgccgttgcc gcactgcgcc acttcactgc catcagcatt
     6661 gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca ccagcaattg
     6721 gtcaaatcct acccccaggt gccgatccgc caggcgacga atcagatccg gtgaaaaaaa
     6781 gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat gcattttcga
     6841 gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt aattcacctg
     6901 ggatggacca tcgccagtgg cgcggtcgtt tttatccggc accgtggatt gcgtttgcgt
     6961 ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat agagcggacc
     7021 tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg ccttaaacac
     7081 gtttttcatt atggattgcc tgtaaagttc atcgctttct gctttctatc atcgcaggag
     7141 aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc tgaaaagtga ggaattgctg
     7201 cgagtataac gcttacacgt ggcgatttat gttttgctgg tagtttcgtg atcggcacct
     7261 cgcgccagga tggctttatg cactatcgca atgtggtttg tttattgtct tgttcgttgt
     7321 tcttatcgtc ggcatgggga tgtcggcttg atgagccgga acataatatt taccagaaac
     7381 agggaaaagg cgtggtgtac ctgcgccctt atgaaaagac caatctttcg ctaccgcaga
     7441 ttaactacaa gcgtctgcgt ctgttaccga atttgctaat tgacccaacc aaactggagg
     7501 attgggaaac ggtgccccca gctaccaatc tcatgacaga cgttgtctac agcggcgcaa
     7561 acgcaactct tccccattac tcttactata gcgatggtcg cgctattctc tacgctggcg
     7621 agattgtgca aaaccctcca ggcacaccgc cagtcgatat ctcgtcattt caggcatggg
     7681 gtgattttgc cgcagataag tacagcctct attacgaagg caaacgcacc gatagcaacc
     7741 agcaactaaa ccgccgaacg ttgcgtcagg tagaatttaa cccgcaatgg aaaccagact
     7801 ggctaggttt gattctccgt gacaagcact atctttacgc aaatggtcag cgccttgatg
     7861 atcccgacac ctttacggta ctggctcaaa agtcatggga tcagcgcggt aaattctcta
     7921 cagcatttaa tccctgcctt cttgccccat ttggcccctg ggatacgcta gcccgcacac
     7981 agacgaaaat catgatcaac ggagaacaac tggatgccga cccggacacc ttttccgtcg
     8041 tgcgctggat gcccggctca ctcttgacct ggcgtgataa aaacgggtta cagcgtaaag
     8101 tcctcgacaa ggaaaatctg gcgtgggatg aggatctaac aaagcactgt ctggattttt
     8161 ctctgctgga aaagaaagtg ttctggcgtc aggggcctga ctgtaaacag gaagaattac
     8221 cgggactcga tccggaacag tttcacccca tcagtgatgc tgtcgcccag tatcaggact
     8281 cgctttatac catcatcgaa acagagtcag gtgaccgcaa gttggagatc gtgaaacttg
     8341 atgatcccaa tcttattatc aacaaacgtt tcaacgccgg gaaacgccac ggctatttac
     8401 ttacgcgtgc cgaagggtgg ccataccatt ccggtttaca cgtgtttgaa tctgacggac
     8461 cgctgatctt actggataac cgttctccgg atgaacgcga agcccatctt aatgaccatc
     8521 cctttttgcg cagatggtat gcccgcgata accgctacgt ttacagcttt gatggcgcgc
     8581 agctctggcg ataccgcact gcggatccga aacaagttcg cttaatctgg aaggaacaac
     8641 attcgggata tggctacggg gtaaattaca aaacgggata tctggacgga aaaattaccg
     8701 atgacggcga atttattcct gccccgcgca atgaggcgac aaaatgaatg gtcaaataat
     8761 cactgttgca ctcatcctgt tggggcacat atttctgacg cctgctgtac aggccatagg
     8821 gttcgactat tacaatgatc acggtgttat gtcttacggt aaaggttatg gggaggatga
     8881 gaaaatcatt gcccaatttc cgaagatgaa cagagccgat ttgcgccttg tgacaaatat
     8941 atccggggag cgggagttgg tggaaggcta tatacccacc gatgaaataa gcataaaaaa
     9001 tgaggaatac cattgggtga cagacggccg cgttattctc tggcgtggca aaatcgttag
     9061 caatccaccg ggaacaccca ctgtcgatat tgccagcttt caggctatgg gccgcttcgc
     9121 ggtcgataaa tatagcctct attttgacgg acagcgcacc gaaagtaata gcggcgcgtc
     9181 acgtgttgat ctggcgacac taaaagctat cgaaggtaac tctaccacgc tggtggatag
     9241 taaaaatctc tacttgtccg gtcgacgtca ggggagcagc agtaatgtta ctgtattaga
     9301 aaaaagatgg tggggtatta atccacgtct tatgagtgta aacagaaatt tgtattccaa
     9361 tgatctgctt atccgcagtg ggcagaatat ttatttaaat ggcgctcacc ttacggcgaa
     9421 tgcagactca tttgagataa ttcgctggat acctcattca ctgctggttt ttcgcgacaa
     9481 taagggtctg catcgttatc cctttggtca attatctggc aaagcgatac ccgtagatga
     9541 tgacgtctct tttgaagtag gggaaagtcg cgttcgctgg cgtaaacagc tcacgcccga
     9601 ccgtcagtgg agccagtgga tagacctaca aggtattgaa cctgaacaat ttcatctgat
     9661 tactgacaat attgcgcagt ataaagatcg gctgtatgta acaaaattat cgacttttgg
     9721 tgaagaccag cttgaaataa tcccgctgga ttcgccagac ctggtcattg atcactcatt
     9781 taatcgcggc aaacagcatg cttactttat ccaccaatta cggtcacgca agagcgtgca
     9841 aattattcca actaacaacg gcccgctaac taaaaacgat cgctttgctt atgacgatcg
     9901 caatgtttat acatggaccg atacagaggt aaggattacg ccctccccct gcccggcgaa
     9961 aacacgcgtc agagaagaga atgtgcatga acttcacaac agagacatta ttattccgtt
    10021 gacggatgaa tcatgccaga acgcagcaga cgagcaaact ttgaagccct gaccccatgc
    10081 cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag tgaatttcat
    10141 cgcctggctg atcaactctg gctgaccatt gaagaacgcc tggacgactg ggatggcgac
    10201 agcgatatcg actgtgaaat caacggcggt gtactaacca ttacctttga gaacggcagc
    10261 aaaatcatta tcaaccgcca ggaaccactg caccaggtgt ggctggcaac caaacagggt
    10321 ggctaccatt ttgatctgaa aggcgacgag tggatttgcg atcgtagcgg tgaaaccttc
    10381 tgggatttgc tggaacaggc ggcgacgcag caggcgggtg aaacagttag tttccgctaa
    10441 gattgcatgc cggataagcc tcgctttccg gcacgttcat cacgaaaaat actgctgcaa
    10501 cagcggcgcg tcatgctcct gattggcagg tggcatgtta ccgatagatt tggtgcgaaa
    10561 cggaatcacc tgttcacgac catcaacctt cacaatctga tagaactgcg gcaggttgaa
    10621 gttgatgaag cttgagccgt aggtgaaacg gtcgtgcgaa gacgagtaga agcgactgac
    10681 gtcacgcacc agttcctctt tgctgccttc gcagtggtga tacacctcaa cccggttgct
    10741 ttcgtcgaga atgtagatat taaagccatt ctcgtcttgc gtttcttcga agaaaaactg
    10801 gatgatccct tcgctggcaa agccgtccac caccgccggt aatttgacgt gattggtttc
    10861 aacctgcact gacaggccgt gcagtttgtt atgcgaaata gcgccataaa actcgatggc
    10921 gttttccagt ttctgtaccg agacattcag ccgttcgaag aacaagcccc aggtttgacc
    10981 ggagacgcgc agcgccttga agcgcccggt ttcctggcgg gtgctggaaa gacgcagttc
    11041 aatacactca gaaaccagtt gctgcacgcg agtacggatt aagccgcgca gatgctggct
    11101 ataacagaag acttccacgc tatctggcgg tgcggcgtcc tgatgcattt tgccgagaat
    11161 agttttcagg gcttcgatca tcgattgctc gccgttgaag tgcagcgtac gcacttcgtt
    11221 ccacgagttg cgatacagca ggtcaacgct acctaccagg caattttgat tctcgccaaa
    11281 gctgaagaca tccagcttac ggaaatcaaa atgcaccacc tgattgcgga acgcagctgt
    11341 cgggtcatat tccaggttaa cgataatcgc cagatggcgg atctcacacg ggctgtagag
    11401 cgccttcggt gtcggtgcag gtaagcgcag cgggaaatgg tgcgacacgt cggcgaccat
    11461 ctcctgcaac ttaggcaaat cgacaatgcc gttaccttta atatacaagc gggtgcgcga
    11521 ggtcagcagg ccgttaaacc atgcccacgc caccagttta ttcaggtaac ggttatattc
    11581 cagcggctga tggctgatga tcgactcaat atttggcgcg cggttataca gataccaacc
    11641 tgaacggtta gcccggcccg gcggcacata aataaaggtc agattcggtt ccgagagatc
    11701 gggtgaaatc tgcgggttta ccagcgtcac tttacccggt aacgcttcaa acgcggcgta
    11761 cagtttacgt gtcagaacac cgatatcctg cgggctggcg gaaacgctaa ggttattgcg
    11821 acgcgcaaag cggatcagat tgcggtagct ctgcatcatc gcgtcgagca actcgttgtg
    11881 cgcctcacgc acctgatcta tcttccagtt agcgcggtta tcaagcattg ccagacgaga
    11941 atcgtcccat tcccactctt tcaccaactg gcttaacact tcacgacgcc agcctacgca
    12001 ggcgcgttca cggctgagtt tttcgcaaac ttttaaatag aagcatcggc gaactaaatc
    12061 cagacgagta aaatcttcga tcgccgtcag atattcagtg acgcgctcca gcatcatgca
    12121 atacgggtcg agaccaaacg acactatttc gccgtcgtgc aaacgctgtt tgatatcttt
    12181 cgccagcaga cgtgggttcg ggtattccca ggaataggct tccagcagca gtgttttcag
    12241 caccgctttg tatggggaat cgatactctt gtagagctgc caaaggctgg caccaaagta
    12301 ctcttcagca gaaagcgagc ttaagccacc aagatccagc cattcatttg gcgtcaacac
    12361 gccctgcgcg taaagcgtca tcacatagtc gtcgtaatgc tcttcttcgt cgcacggcac
    12421 catattccac agaatacgct taccggcgag acgcacggcg gtacgataaa attcgtcaag
    12481 cagcagtata tgctgggtgg agccacaatc ttcgcccccc aggctgccgc tttcattatg
    12541 acggaagcgg ttttcatcaa tcaggaagaa gctgacttcc acacccagcg aggcggccca
    12601 gctttccagc agactacatt tacgttgtag caactggcgc tcttcgctat cgagccagga
    12661 ttgatgacag acccagatat ccaggtcaga ggaacaactt tgccctacgg acgaggtgct
    12721 gcccatggtg tatacaccag taattggaag ctcacctttc ggtgggtcct gtactgacat
    12781 tccacgatac agttcaagct cgttcaggta gtggcgttga gtttcatcag gcgtgtaaag
    12841 gcaaatgcct ttgggaacgt taccatcaag gtaacccggc attagcggat ggtgatagtg
    12901 caacaatgtc ggcagtagac tgtagacctg ttggaatgca ggccccatag cagcaagcgc
    12961 gcgatccaca cgcaattgat ttatggcatc cagtctctgt ttcagagtct caatatagag
    13021 gtacaagacg tatcgcctga tttgctaccc gtcatgactg tgattccgcc aacatcaacg
    13081 gtaacacgcg gcattcggga tatttcgtat gtcaaaggta accgttacca cttttcgcgc
    13141 ctggtttttt tagtttcacg acgaaaaaat ggtctaaaac gtgatcaatt taacaccttg
    13201 ctgattgacc gtaaagaaag atgcgctaca tacaagtgta gcaccgttta ttctctgtaa
    13261 attccttatt acgaaggctc aaagcgcctg tcaggatcca ctgccagacc tcattttacg
    13321 gtttgcgcag gcgtctacgt ttcaccacaa cactgacatc gctctggcaa ggatgttagg
    13381 atggaccacg gatgataatg acggtaacaa gcatgttaga caatgtttta agaattgcca
    13441 cacgccaaag cccacttgca ctctggcagg cacactatgt caaagacaag ttgatggcga
    13501 gccatccggg cctggtcgtt gaactggtac cgatggtgac gcgcggcgat gtgattcttg
    13561 atacgccgct ggcaaaagta ggcgggaaag gcttatttgt gaaagagctg gaagtcgcgc
    13621 tcctcgaaaa tcgcgccgat atcgccgtac attcaatgaa agatgtgccg gttgaattcc
    13681 cgcaaggtct ggggctggtc actatttgcg agcgtgaaga tcctcgcgat gcatttgtgt
    13741 ccaataacta tgacagtctg gatgcgttac cggcaggcag tatcgtcggg acgtccagtt
    13801 tacgtcgcca gtgccaactg gctgaacgcc gcccggatct gattatccgc tccctgcgcg
    13861 gcaacgtcgg cactcgtctg agcaaactgg ataacggcga atatgatgcc atcattcttg
    13921 ccgtagccgg actaaaacgt ttaggtctgg agtcccgcat tcgcgccgcg ttgccacccg
    13981 agatttctct tccggcggta gggcaaggtg cggtgggcat tgaatgccgc cttgatgatg
    14041 cacgcactcg cgagctgctc gccgcgctga atcaccacga aactgcactg cgcgttaccg
    14101 ccgaacgcgc catgaatacc cgtctcgaag gcggatgtca ggtgccaatt ggtagctacg
    14161 ccgagcttat tgatggcgaa atctggctgc gtgcgttggt tggcgcgccg gacggttcgc
    14221 agattattcg cggtgaacgc cgcggtgcgc cgcaagatgc cgaacaaatg gggatttcgc
    14281 tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc tataacggag
    14341 acgccccggc atgagtatcc tggtcacccg cccgtctccc gcaggagaag agttagtgag
    14401 ccgtctgcgc acactggggc aggtggcctg gcattttccg ctgattgagt tttctccggg
    14461 tcgacaatta ccgcaacttg ctgatcaact ggcagcgctg ggagagagcg atctgttgtt
    14521 tgccctctcg caacacgcgg ttgcttttgc ccaatcacag ctgcatcagc aagatcgtaa
    14581 atggccccga ctacctactt atttcgccat tggacgcacc accgcgctgg cactacatac
    14641 cgtaagcgga cagaagattc tctacccgca ggatcgggaa atcagcgaag tcttgctaca
    14701 attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg gcaatggcgg
    14761 tcgtgagcta attagggatt ccctgacggc gcgcggtgct gaggtcactt tttgtgaatg
    14821 ttatcaacga tgcgcaatcc attacgatgg tgcagaagaa gcgatgcgct ggcaatcccg
    14881 cgaggtgacg acggtcgttg ttaccagcgg tgaaatgttg cagcaactct ggtcgctgat
    14941 cccacaatgg tatcgtgagc actggttact acgctgtcga ctattagtcg tcagtgagcg
    15001 tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg ataacgctga
    15061 taacgatgcg cttttacggg cattacaata actctcataa caggaagcca taatgacgga
    15121 acaagaaaaa acctccgccg tggttgaaga gaccagggag gccgtggaca ccacgtcaca
    15181 acctgtcgca acagaaaaaa agagtaagaa caataccgca ttgattctca gcgcggtggc
    15241 tatcgctatt gctctggcgg cgggcgtcgg tttgtatggc tggggtaaac aacaggccgt
    15301 caatcagact gccaccagcg atgccctggc taaccaactt acggcactgc aaaaagccca
    15361 ggagagccaa aaagccgagc tggaaggcat tatcaagcaa caagctgtac agcttgagca
    15421 ggcgaatcgt cagcaagaaa cgctggcaaa acagctggat gaagtccaac aaaaggtcgc
    15481 caccatttcc ggcagcgatg ctaaaacctg gctgctggcc caggccgatt ttctggtgaa
    15541 actcgccgga cggaagctgt ggagcgatca ggacgtcacg accgctgcag cgttgctgaa
    15601 aagtgcagac gccagcctgg cggatatgaa tgacccgagt ctgattaccg ttcgccgggc
    15661 aattaccgat gatatcgcca gcctttctgc ggtatcgcag gtggattatg acggtatcat
    15721 ccttaagctt aatcagcttt caaatcaggt agataatctg cgtctggccg ataatgacag
    15781 cgatggttcg ccgatggatt ctgacggtga agagctttcc agttccatca gcgaatggcg
    15841 tatcaatctg caaaaaagct ggcagaactt tatggacaac ttcattacga ttcgccgtcg
    15901 tgatgacacc gccgtaccgc tgttagcgcc aaatcaggat atctatctgc gcgaaaatat
    15961 tcgctctcgc ctgctggtcg cagcacaagc tgtaccgcgt caccaggaag agacttatcg
    16021 ccaggcgctg gagaacgtct ccacctgggt acgtgcttac tacgatactg atgatgccac
    16081 caccaaagcg ttcctcgacg aggtggacca gttaagccag caaaatatct cgatggatct
    16141 tccggaaacc ctgcaaagcc aggcgatgct ggaaaaattg atgcagaccc gcgtgcgtaa
    16201 cctgctggca caaccggcag cagggacaac ggaagctaaa cctgcacctg cacctgcacc
    16261 tgcacctgca cctgcacctg caccgcaagc tgatgctccg gcagccgcgc cgcaaggaga
    16321 ataaccatgc taaaagtgtt attgctcttt gtgttgctga ttgcggggat cgtggttggc
    16381 ccgatgattg ccggccatca gggttacgtg ctgatccaga ccgacaacta caatattgaa
    16441 actagcgtca cgggcctcgc gattattttg attctggcga tggtagtgct gtttgccatt
    16501 gagtggctac tgcggcgaat cttccgcaca ggcgcgcaca cccgtgggtg gtttgtcgga
    16561 cgtaagcgtc gccgtgcacg taagcagacc gaacaggcgc tgctgaaact ggcggaaggc
    16621 gattatcagc aagttgaaaa gctgatggcg aaaaatgccg atcacgctga acaaccggtg
    16681 gtgaactatc tactggcagc cgaagccgcg caacaacgtg gtgatgaagc acgcgccaac
    16741 caacatctgg aacgcgcagc ggagctggcc ggcaatgaca ccattccggt agaaatcacc
    16801 cgtgtacgtc tgcaactggc ccgtaatgaa aaccatgcag cacgtcacgg cgtggataag
    16861 ctgctggaag ttacgccacg ccatccggaa gtattacgtc tggcggaaca ggcgtatatc
    16921 cgcactggtg catggagttc gctgctggat atcatcccat caatggcgaa agcccatgtt
    16981 ggtgatgaag aacatcgtgc aatgctggaa caacaggcat ggattggcct gatggatcag
    17041 gcgcgtgccg ataacggtag tgaaggtttg cgtaactggt ggaaaaacca aagccggaaa
    17101 acgcgtcatc aggtggcctt gcaggtggca atggcggaac atcttattga gtgtgacgat
    17161 catgatactg cccagcaaat tatcatcgac ggcctgaaac gccagtatga cgatcgcctg
    17221 ctgctgccga tcccgcgtct gaaaaccaat aacccggaac agctggaaaa agtgctgcgc
    17281 caacagatca aaaacgtggg cgatcgcccg ctgttgtgga gcacactggg tcagtcgctg
    17341 atgaaacacg gagaatggca ggaagcatcg ctcgccttcc gcgcagcgct gaaacaacgt
    17401 ccggacgcct acgattacgc atggcttgcc gacgcgctgg acagactgca caagccggaa
    17461 gaagccgcag cgatgcgtcg cgacggtttg atgttaacgt tgcagaacaa cccgccacag
    17521 tagctctttc tcacccggag gcaagcacct ccggggcctt tctgatatat aaaaaaacgc
    17581 ctgctcttat tacggagcag gcgttaaaac aggtctgtat gacaacaagt gggtgcttca
    17641 ctcaacgttg tgtccatggt gtctgatgag gcataagcga catctgtcag tggacgataa
    17701 gcaccgtaaa cggctctgcg tcattccgga gtttatgagg cactaaggcg aacataagag
    17761 atggaatgag catctactct tttattatgc cacagagaac cgggaaataa catcccttaa
    17821 cacttgttat gagataattc tgtaatcctc tttacttcct gagtaataac ttcctgagtg
    17881 aatatttctc ctgcgcacaa ttctacacat atatataatg aataataaaa gtcattcaat
    17941 taataacata tatattaatt gccgttaaaa ctaaaaacag catcaataat caacgcgata
    18001 taacatcacc gccttacata tcacctgcgc cagaggtagg attgaaaatg ctctcctgat
    18061 ttttcaattc attttctgga taaataaata atttattttt atcactatta tttatgcaat
    18121 catcctgtca gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgacga
    18181 catgaaaccg gagcattttg catggaattt tcgttttcac ccaaacttct tgttgttgct
    18241 gtcgccgcag ctcttcctct catagccagc gcagcagata ccccgtcaac cgccaccgca
    18301 cgcaaaggct ttgccggata cgatcaccca aaccagtatc tggttaaacc ggcgaccact
    18361 attgccgaca acatgatgcc ggtgatgcag catcctgcac aagacaaaga aacccagcag
    18421 aaactggcgg aacttgagaa aaaaaccggt aagaaaccga acgtggttgt tttcttgctg
    18481 gatgatgtgg gctggatgga cgttggcttt aacggcggcg gcgtggcggt gggtaaccct
    18541 acaccagata tcgacgccgt tgccagccag gggctgattt taacttcggc gtattctcaa
    18601 ccgagttctt ccccaacccg cgccacgatt ctcacctgac aatactccat ccaccacggc
    18661 attctgatgc cgccaatgta cgggcaaccg ggcggtctgc aagggttaac cacgctgccg
    18721 cagttgctgc acgatcaggg ctacatcacc caggctatcg ggaaatggca tatgggggaa
    18781 aacaaagagt cgcagccgca gaacgttggc tttgatgatt tccgcggctt taactcggtg
    18841 tccgatatgt ataccgaatg gcgtgatgtt catgtcaacc cggaagtcgc cctgagcccg
    18901 gctcgttctg aatacatcca aaaactgccg ttcagcaaag atgacgttca tgccgtgcgc
    18961 ggtggtgaac aggaagccat tgccgacatt acgccgaaat atatggaaga cctggatcaa
    19021 cgctggatgg aatatggcgt taagttcctc gacaagatgg cgaagagtga taagcctttc
    19081 ttcctctatt acggcactcg cggctgtcac ttcgataact acccgaacgc caaatatgcg
    19141 ggtagctctc cggcgcgcac ctcttacggc gactgcatgg tggagatgaa cgatatcttc
    19201 gccaatctgt ataaagcact ggagaaaaac ggtcagcttg ataacacgct gattgtgttc
    19261 acctctgata acggtccgga agccgaagtt ccgccgcacg gtcgcacgcc gttccgcggt
    19321 gcgaaaggtt ctacatggga aggcggcgtt cgtgtgccaa ccttcgttta ctggaaaggc
    19381 atgatccaac cgcgtaaatc tgacggcatt gtcgatctgg cggatctctt ccctacggcg
    19441 ctggatctgg cggggcatcc tggggcgaaa gtagcgaatt tagtaccgaa aaccaccttt
    19501 atcgatggtg tggaccagac atctttcttc ctgggaacaa atggtcagtc taaccgtaag
    19561 gccgagcatt acttcctcaa cggtaaactt tctgctgtgc gtatggatga gttcaagtat
    19621 cacgtcctga ttcagcaacc ttacgcttat acccagagcg gatatcaggg tggattcacc
    19681 ggcacagtaa tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga tccgcaggaa
    19741 agcgactcta tcggcgtgcg ccatatccca atgggtgtac cgctacagac cgaaatgcat
    19801 gcgtatatgg agatcctgaa aaaatatcca ccacgcgcgc agattaaatc tgattaagcc
    19861 ggcgctatcg ctgagatcta cctttgccgg atgcgatgct gacgcatccg acaatcaacc
    19921 gcaggcggcc acaaaatggc tactttttta cttattcacc accagcaaat gcgcctgcat
    19981 aatgtcgctg gccggacgac cgtgcgccag caaatcaacc attgctttaa gatatggcgg
    20041 tagatggcgg aaataacgct gatacccggc acacaaataa ttcagcccgg gtttgccgct
    20101 ggcatcgagc ataaagcggt gtttcgggca gcctccccag cacgctttta acacgttgca
    20161 actgcgacac tgtgccggta actgcttgaa tttatcttca ccaaatgcct gctgttgcgg
    20221 ggaatcaatc atttctgcaa tcatctgctg aagcatattc cctagtcgat actgcggata
    20281 aacatagtga tcgcaggcat aaacgtcacc gttgtgctca acaatcaccg agcgcccaca
    20341 ggttggctga tgatggcaaa ccgcgcccgg tgcgccgaca aaattggcga acgcccattc
    20401 gatattcatt acgaaaatct tgccgacgtc gcgcttaatc cagtggtcga aaatcgccac
    20461 cagaaactca ccgaactctt gcgggcacac cgaccattcc gtcagttcgc cctgaatatc
    20521 gccgggagca tgtagcttaa gtccagcatg agcagccgtt tcatcagcca ggcgctcgac
    20581 caccggaata aactggatga actcaacacc tgcatcacac agaaaatcat agacttgcaa
    20641 aggttgtaac gcactggtgc gattgacgca gaccagcacg ttatagtcga catgatgttt
    20701 ttgcaggagc gacagggcac gcatcaccag cttatgcgtt ggtctgccac ctttggtcac
    20761 gcgatattga ttgtggatct cagccggacc atccagcgat aaccctacaa gaaaatgatt
    20821 ttctgccaga aatgcacacc attcgtcatc gagcagcacg ccgttagtct ggaagctatt
    20881 acttatcttc ctgccagcac catatttcgc ctgtagcgcc acggcacggc ggtaaaaatc
    20941 cagcccgagt aacgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgggtttc
    21001 gctggcagcg atatagtgac ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac
    21061 tggcttttcg tggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt
    21121 acaatcagaa ccgctcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgctg
    21181 catggtcgct ccttgcctga agtcatcctt caataagtta agggcgaaat cataccgtgg
    21241 cgcaatgcgt tattacgggc gtttattgcg aagggaaagt gacgaaatgc acagaaaaca
    21301 aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac
    21361 ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt
    21421 gaatttttag ttcaattctt taaagtcgtg gtgcgagggg ggggacttga acccccacgt
    21481 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt
    21541 cacaacttct aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa
    21601 tccagggctc taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact
    21661 accaccgcag ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac
    21721 ctctgcctcc ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg
    21781 ggggtggata atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg
    21841 tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttaccgtggc
    21901 gattaagccc aaaaacttta taaatcgccg tcaccaccag cataaagatg atgccgacaa
    21961 acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg
    22021 ccattgttac gtagttggcc caagggaaca ggattgagcg gaacggatgg ctggcaattg
    22081 ccgctttgtg tgcacggcga aaacgcagct ggcttatcaa aatcacaaac catggcacca
    22141 tacccggaag cacgctggcg ctgtagacgt agacaaacac acgctgtgga ttgggaatga
    22201 tgtagttcag gcatgaacca attagcagaa tagcaataga tactgccaca cccgcaaccg
    22261 gtacgccgtg acgggaaact ttcgccattg ccgccggtaa ctgacggttt ttcgccagtg
    22321 catagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca
    22381 gcaccacaaa gttgataatg cccgccgctg cggtaatgcc gattttggcg aaagtcagta
    22441 cgaacgggct accgttgctg cctatttcat tccacgggaa gatagtaacg ataacgaaaa
    22501 tcgcgccaac gtagaaaatc aggattcgcc acagcacctt gcctacggca ctgcgcagcg
    22561 tcacctgcgg attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg
    22621 acgccaccac aatacacaat gcggtcagga accctttcca gccaccagca aagaaaccgc
    22681 catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa
    22741 tcacgcccag gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc
    22801 agaactcgat ttcgccgtac aacctcaccg cagccagatt cgccaacgcc accagcgcca
    22861 ccgcgatcaa tgcgggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc
    22921 caatggcggt gatttctgag atccccaccg ccatccacat aaaccagtaa gaccaggcgg
    22981 tgagataacc aaagaacggg ctcatataac gatgcgcgta aacggcgaac gaaccggtaa
    23041 ccggttcgag gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aacagcccgg
    23101 cgatgatata ggccaacaat acggatggcc cggcccattt cagggtactg gcggccccca
    23161 taaacaggcc gacgccaatg gtgcccccca gggcgatgag ttcgatatgt cgagcttcca
    23221 gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct
    23281 ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc
    23341 aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac
    23401 aggcgacgaa gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg
    23461 taatgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa
    23521 agcaggcggt agagccactc cagccccagc gtttgccaga ttttcggtgc gcgttttacg
    23581 tgaccggtga aaacatcgta ggtcccgcca acgcccatat acagcgcatc tggatgcacc
    23641 agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt
    23701 tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa
    23761 ccatcctgac tgccaacgat attcacattc cactggttgc gcagtttagc ttcagtttgc
    23821 gccagcactt caggtttacc gcccacaaga aataccggtg tcccttcttt gcctgcgcgc
    23881 gccatcagct cttcccagag atcggcccca gcaacgcggg aaacctgcgc ctgcgggtac
    23941 tttttacgta ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta
    24001 attaactccc tgacctcggc gttatcttca atggtcagca ttttttcggc attaatggca
    24061 accagcgttc cctgcttaag ctgcccgtca gcgaacagat aatcgagggc atgctgcata
    24121 tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta
    24181 ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgttcgttta tgaatgagtc
    24241 cggcgctttc aaaaagccag tacaacagtt ttgcgatcat cagacatgcg ccgaagacca
    24301 cgataaaaaa gaccacgcgt gagacaaacg aatccagccc ttcacgcgcc agcacgatca
    24361 tattgaagat cgccccgaag cagaaactgt gcaatatcgc agccttatag cgattagtct
    24421 cgcggttgcc cagctcatac agccagtcga accatttgat aatcagtcca accacaatcg
    24481 ccccgagcgg gatgaacaac gcgccgccca taacaaccag tgagcctata agcgtaggcg
    24541 agatcgccag tccggagtgg ttattcagca cttcccaggt aaagtagttg gcggagttca
    24601 gcaccatact cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga
    24661 caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat
    24721 tctcccacgg cgagaaagtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc
    24781 cgctcacatt cattccatag cgttttagcg ccagccagaa catgcccaca atccccagca
    24841 cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga
    24901 agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcatg taagtcaaca
    24961 agccaaaggc aaccgtgctg acgaggaaaa acagccacgc tttgctatcc tggcgcagaa
    25021 aataaaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaat gccacgccgg
    25081 agacttcgct ggaaaagatc tgactgtaag aattaagccg gaacagcaaa aagccgttgt
    25141 gcatgaagaa gatgccgacg ctcaccagag cgatgcccat caggatcacc cacgtgagat
    25201 tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tgcatcagca acgcgtttgc
    25261 gtagacgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcctgca gaaagcaacg
    25321 cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg
    25381 tcagcggaaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt
    25441 taaagcgcac acggcgaaac tcaaaccagg tcagcgtggc aataaacagc gtgcagagca
    25501 gccagacaac aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat
    25561 cgccaacgcc cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt
    25621 atccaccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcatcggt
    25681 agtaaacagt actggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt
    25741 aagcacgcaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg
    25801 ggcaaaaata aagtaaccga gatcgcactg gcgaagtagc gtcagatagg cgtcaaattc
    25861 cagtttttcg ctcagaactt gtaggttttc ttcgctgaat aactccagtc ccgcctgacg
    25921 aacttcctcg atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac
    25981 cgtatcgcca aattgctgat gaacggcgcg caaggcagca atatgctcat tgctgcggtc
    26041 accggagttc cccaccagaa tggtcatttt tccttcacgt tgccgatcgt tcgccatcgt
    26101 attaagagaa gggtccatcc gcgtcgggaa gtacagcagt tctccccgca cctttggatg
    26161 cgttttggca aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctctcttttg
    26221 cgccaggcga cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag
    26281 gtctgctccc cagatatgcc agaaaaactg gctgggctta atgccaccac tcagcagagc
    26341 cagccacagt ttgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt
    26401 tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag
    26461 cgccggacag ctatcactta agccgtcgtc cttgccagca accataaact cgcgcgcgtg
    26521 cccgctcgtc gcggccagcg cgtcgttgaa aaaccgcaga acggttcggt tatggtgagg
    26581 gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac
    26641 acgccacaac aaagagaaaa atagacgata taggttgcca tataggcctg tgctgcgccc
    26701 agtgcgccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg
    26761 atttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca
    26821 taagcgccca cttttaacac atcacccacc aactgccagg caaagagatc gcgcatagcg
    26881 gtaaatttat tcgacaacag cagccagata gcaaaatcac gcagcaacca gacggtaaaa
    26941 ctcgccgccg ccactgccgg taagacgaat ttcagcgatt taaccacttc ccgggtgata
    27001 tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta
    27061 ataaattgca ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc
    27121 cagctatact gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc
    27181 gacgtaatca acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag
    27241 ctgggtttca gatagcttaa cgggatgaca ccacgtttga tcaacatgat aacagcagga
    27301 attaccacca gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa
    27361 cgataactga cgtagtacgc gagaacgcca atcaagctac cgacaatcag agataacgca
    27421 ttacctgcgg catcgcgaaa gcctttcatc aacgccagta acaggttgcc ccaggcaatc
    27481 cccatttgca ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaac
    27541 ccctggctga ttggcgcagc tgccagcaca aaaaccagcg ccattagcgt agagaagcca
    27601 agtaccatcg ctgatgaagt gccgaccaca cggcgcagct gttggggatt atcatgatac
    27661 tgggcaacgt atttggttac gccgttaaag atgcctgccc cggcaagcac gccgagcacg
    27721 gtaatcaact ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacactgcc
    27781 agcaacttac cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac
    27841 aaggacgctt ttgccaacga catatcagga gaagtagttc aacaaggtcg caattaccgt
    27901 acgctgattg acgggcgaca ggttgtagaa caacggcagg cgcagcaagc gctcgctctc
    27961 tttggtggtg tagcgatctt cgccgtggaa ctcaccaaag cgttcccccg cagggcttgc
    28021 gtgcagcgga atgtaatgga acaccgccat gatttccgct tccttcagga agctaatcaa
    28081 cgcgctccgg tcatcaatat cccgcagctt gatgtagaac atatgcgcgt tctgcacgca
    28141 gtcatccgga atcgacggca gctcgatacg cccggcttcc gccagaggtg ctaacgcatc
    28201 gtagtagttt tgccacagcg ccagacgttg ctggttgatg cgttcggctg cttccagttg
    28261 cgcccacagg tacgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca
    28321 ggtatattta tcgacctgac cacggaagaa ctggctgcgg tttgtgcctt tttcacggat
    28381 gatctcggct cgttcgatca acgctttatc gttaatcagc gtcgcgccgc cttcaccacc
    28441 cgccgtgtag tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag
    28501 cgcacgccct ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaacagatt
    28561 atgctttttc gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac
    28621 cggcacgata acgcgcgttt tgtcggtgat cgccgcttca atcaacgttt cgtcgatgtt
    28681 catggtgtcc gggcgaacat ccacaaaaac gatttttgcg ccacgcagca caaaggcatt
    28741 ggcggtggag acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag
    28801 cagcagcgcc gccatctcca gcgaagcggt gcaggatggc gtcagtaaca ctttggcgct
    28861 gccaaaacgt tgctccagcc actgctggca gcgacgggta aaaccgccat cgccgcacag
    28921 tttgccgcta cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc
    28981 gttaaatgga atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac
    29041 cactttgtat atatcgttta agcgcggcgg tgttgcccat ctgggtcgcc acccgcaaag
    29101 ttattttacc gcgagcatac gcccagttta gcgccgtttg cataagctca gcacctgcac
    29161 cgcgtccagc cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat
    29221 aaccgcgaat atcgccggac gccgcacgta aaatcagaca ttgatgatca aaggtgccgc
    29281 gcacggcatt ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg
    29341 gcgcacgaaa acggctttgc gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat
    29401 ctgtctcttg tgccacttct gcgccgctat caccgacatt gttcacgggt agcgccaaat
    29461 caacttcacc ttctaccagg gagaatccca gctgttgcag ggcatccagt tcacccgtat
    29521 ttgatgccgc aattttggcc tgcacccgtg accacggcgc taacgcgtct ggcgtcagga
    29581 gcggtgcttc agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc
    29641 aggttagtgg ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg
    29701 tagccagttt tcgccagcga gctggcggcg cgcttcacgc cttcgtcatc aagccagccg
    29761 ttacgccagg cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc
    29821 tgcacaaaag tgctggcctc aatcaggctg tcgtgagtgc cggtatccag ccaggcaaat
    29881 ccgcgcccga gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag
    29941 gtaatctcca gttcaccacg ctccgacggc ttcacctgct ttgcgtactc cacgacttta
    30001 ctgtcgtaga aataaagccc ggtcaccgcc cagtttgatt tcggctgttt tggtttttct
    30061 tccagcgaga tagcgcggaa attgtcgtca aactccacca cgccaaagcg ttccgggtcc
    30121 atcacctgat agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc
    30181 ggactgaagc cctgtccgaa gaagatgtta tcgcccagca ccagacaaga aggttcaccg
    30241 ttgaggaagg tttcaccgat gataaaggcc tgcgccagac cgtccggact gggctgttcg
    30301 gcgtattcca gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct
    30361 ttatcttccg gcgtagtgat gatgagaatt tcgcggatac cggccaacat cagcaccgac
    30421 agcggatagt aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc
    30481 gtaatcggat gcaatcgggt gccggaaccg cccgccagga taataccttt catgtttgcc
    30541 tccgctgaaa attagccttt cagacctaaa cgctcgccct gatagctgcc gtcctgcacc
    30601 tgcttacacc agctttcatt agccagatac cactgcaccg ttttacgcat tccactttca
    30661 aaggtttcct gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa
    30721 cgcagatcat gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg
    30781 ccgtgcggct tattaggagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg
    30841 agattcttac gctcgttgtg accaccaata ttataggttt caccgacttt ccctgtggtc
    30901 gccacgcaat acagcgcgcg ggcgtgatct tcaacataca gccagtcacg gatttgctgc
    30961 ccgttgccat ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc
    31021 agtttttccg gaaagtggta aggaccgtag ttattcgagc agttggtgat aagcgtcggc
    31081 aaaccatagg tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa
    31141 taagggctgc tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg
    31201 tcaccatata cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc
    31261 gtcagcgcag tccagtaagc ccgcgccgct tcaagcagtg tataagtccc gacaatgttg
    31321 gtttcaataa acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga
    31381 tgcatgacac agtctggctg atactcagtg aatacgcgcg ccagttctgc ccgatcgcag
    31441 atatcaactt tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag agacatcagg
    31501 tttccggcgt aggtcagctt atcgaccacc accaccgcgt cgctcgtttc gttgatgata
    31561 taacgcacca gcgccgagcc aataaacccg gcaccacctg ttatcagaat ctttctcatc
    31621 agcgccagac tcctttggca tcgacgacat actgctgatg gacgttgtcg ccattgataa
    31681 ctttgaactg actatgatcg accagcatca ccagcacatc tgccgttgcc agcgcctcgt
    31741 caagctgcgc cagagtacaa agcccggtca gtttcttcgg caactggtgg atgttaggct
    31801 caacaaccag cgtttcgccg ctgtgccacc gggcgatcag ttcagcgatt tccattgccg
    31861 gactttcgcg caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca
    31921 gttcactgac gcgtttatcg gtagcagcca gacaatcagc caccgccgct ttcacctgat
    31981 cgataaccca gaacggtttg tgatcgttca cttcgcgcgc ggtacggata agccgcgcct
    32041 gctgggggtt ctgtgccacg ataaaccacg gatcaacagc gatgcagtga ccgcccacgc
    32101 cagggcctgg ctgaagaata ttgacgcgag ggtgacgatt cgccaggcga atcagttccc
    32161 agacgttaat cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca
    32221 catcgcggaa gctgttttcg gtgagcttac acatttctgc cgtccgcgag ttagtgacga
    32281 cacactcacc ctcgaggaaa attttgtaca gttcgctggc acgggccgaa caaaccggcg
    32341 tcataccacc aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata
    32401 cgcgttccgg gcagtaagca atgttgacgt ccgccaactc gcccacctgc tgtgggaaag
    32461 tgagatctgg acgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg
    32521 aggtggattc aaggatcacc aacgcgcctt ttttcaacac tggcgcgatg gagcgggcag
    32581 ccgattcgac gtacgtcata tctggctcat gatcgccttt aaacggcgtc ggtacagcaa
    32641 tcaaccaggc atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg
    32701 cagtttttac tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg
    32761 tatcaaccgc gtgttggttg atatcgacac caatcacctg tttttgcctt gaggcaaacg
    32821 cagctgctgt aggcaacccg atgtaaccca gtccgataac agaaatggtc gcaaaactca
    32881 tagtgatatc cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc
    32941 atacgggtta tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt
    33001 cacttcctcg acaattcgct gcttatccgt gccgaccaga cgcaccgtac ccgccgtcac
    33061 cgcttccgga cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc
    33121 ttcttcctga atgccgcctg agtcggtcaa aatcagccag gcgtggttca tcagccagac
    33181 aaacggtaaa tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg
    33241 attgaccggc tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg
    33301 gtgcgtggtg gcgatgtctg ccagcgcatg gcagatttct tcaaagccac gaccgaagct
    33361 ctcacgcctg tgaccggtca ccagaatcat ctttttatcg gggtcgataa acgggtaatt
    33421 tgccgccagt tctgaacgca gcgtatcgct gctcatcacc tggtcacgca cccataacag
    33481 tgcatcaatg actgtattac cagtaatgaa gattcggcta tccgcaacat tttcacgcag
    33541 caagttttgc cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa
    33601 tgtacggtta gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc
    33661 aacgtgacca acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt
    33721 agtatcgccg tgaaccagca cgacatctgg tttgaactcg gcaagaatag gttttagccc
    33781 ttccagaatc cgacaggtta tctctgtaag gccctgtcct ggctgcatta tgttgagatc
    33841 gtagtcaggt acaatggaaa agagtttcag cacctgatcg agcatctccc gatgctgcgc
    33901 agtgacgcaa actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggtgc
    33961 catcttgatg gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt
    34021 cgaataagcg gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc
    34081 gggttaatgc gacaccagcc ccgatcagtc ccccgacaat gccccacata atcatcagga
    34141 aggcacgacg tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg
    34201 tctgaaaacg cggatccagg gttggaccaa cattcagggt gtttaacatg gcccgattct
    34261 gatcatagtc gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga
    34321 gcattggacg cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg
    34381 tcgcactgcg cgaaatatta tgctgctcag caattttcag cgcctgttca atactgttca
    34441 tccggcgatc gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct
    34501 ggatggtgcg tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct
    34561 ggctggcaaa agcaacatac tgacgtaaca ggttattagc gtcaggcgca gtttcggcaa
    34621 tcagcttcac gctgtcattg accgcgcggg tgaagtcacc gggaataaac acgatgttgt
    34681 tgatcatttc atccagcaac gccgcatctg ctttgctgtt gcccaccatc cgctgcttgt
    34741 aatagtcggt ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa
    34801 actctttata ggcttcgtcc atgaccgatg gttggtcggc agaagccatg tttgaacgga
    34861 catccaggtt acgcaaaaat tgctgctgtg agtaatatcc ccccagcata ttcaccgttg
    34921 gacgatcggt aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg
    34981 cgattaacgc aaacgccagc cccatgccaa taatccacag cttcccagcc cacaaggtac
    35041 gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg
    35101 gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg
    35161 cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata
    35221 tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg
    35281 agaatattct gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac
    35341 aaacgcctgg cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc
    35401 aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat
    35461 tagcggaatg gcgataatcc acaaagcggt aaccgggctg atgggatgag ttttgccctg
    35521 ggtcgtttcg agcaggatcc agatcacggt aaaaccaatc agcgtactgc ccgcatcccc
    35581 cataaagact ttgtagcggc gacccagtat accaaggtta agcatgatgt atggcaggat
    35641 ggcggcgatc atcgcaaagc accagattgc gagactggtt tgcccgtcga acaacaaaat
    35701 cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc
    35761 aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa
    35821 cggtccgagc accatctccc aggagccaaa gatataaccc aggctactga gataaagttt
    35881 gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct
    35941 gatatcaaaa cggtcatcca ccgcgccaat gaaaacaagc acaccagcac aagcgagata
    36001 gagagatgca tgcggaatat agtaatcaac aattccgaac gtgaagcaaa tccctgcgta
    36061 aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt
    36121 atccactaaa ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa
    36181 taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag
    36241 agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc
    36301 cgaacccacc agccctgtgc aaaaattaac cacttgatat gcaaattgca taacacacca
    36361 gcaaattcca ggaattttcc tagcatcgcc agcgcggcat taagattaca gatataaaag
    36421 caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc
    36481 atttcgaaga aatcgtcatt ggtcttggtc attgccagtt tattaatgag gaattccatt
    36541 gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct
    36601 tcctgagtcg tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg
    36661 aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatattgcc tgtaccttta
    36721 aactcttcgt agataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata
    36781 atggtcaggc tgccgccctc ttccacgtta cgcgccgcac cgaagaagcg tttcggacga
    36841 tgcagggcgt tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacggtg
    36901 ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc
    36961 tcaaccaggc gtttcgcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg
    37021 ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact
    37081 tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca
    37141 atgctctgag caatgttctg cagcagcatg gttttaccgg ctttcggcgg tgccacaatc
    37201 agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc ggttaaatct
    37261 tccgtagaac cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag
    37321 ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact
    37381 ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta
    37441 tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga
    37501 ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc
    37561 agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa
    37621 ataatgtcct gcttacgcat acgagccagg ttttccagcc ccatattttc gccgagagtg
    37681 atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt
    37741 aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct
    37801 gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg
    37861 aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa
    37921 acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca
    37981 acactaagtt agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca
    38041 tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg
    38101 cgcccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac
    38161 ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca
    38221 gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac
    38281 cgcaccactc tgcccagaaa tcaacgagga tcgccccgtc cgctttgagt acatccgtgt
    38341 caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa
    38401 gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt
    38461 caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa
    38521 cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc
    38581 ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc
    38641 tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc
    38701 ttacgtcaac gtttcattat cttctctctc atcccgcgat tgccgatcgc aaagtgaatc
    38761 aaccgcgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg
    38821 cggaaccgct ggcggaagct actggcctga agctgggtct ggcttacggt ggtgatggct
    38881 acgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc
    38941 gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtggtggtac
    39001 tggacgaagc cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc
    39061 gccgtatgcc gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc
    39121 gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg
    39181 aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa
    39241 tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca
    39301 acaccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg
    39361 gtttattgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc
    39421 gtggggatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg cacatccctg
    39481 ctgtgacaca cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta
    39541 ttggtcgaac aggtcgcgct ggcgcaagtg gtcattccat cagcctggca tgtgaagagt
    39601 atgcattgaa tttgcctgct attgagacct atattggtca ctcaattccg gtaagtaaat
    39661 acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca
    39721 caggtaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa
    39781 aaaatgtcat actccaaatc attcaagctg tatcaagatg gcaagagaat gaatcccgat
    39841 gagtttactg tcgtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    39901 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    39961 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    40021 aacgcaaagt gcgtctggct gccggcctga atagcgaaaa tgccctgtct aatgaagcaa
    40081 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    40141 cgcaaattcg cgttgtcgct acggcgacat tacgccttgc cgtcaatgcg ggtgatttta
    40201 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    40261 cgcgtctgat ttatcagggc gttgctcaca ccactggcgg tgccgatcag cgcctggtgg
    40321 tggatatcgg cggagccagt actgaactgg taaccggcac gggtgcacaa accacctctt
    40381 tgttcagcct gtcgatgggc tgcgttacct ggctggaacg ctattttgcc gatcgtaatc
    40441 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    40501 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg cgcttccggc accgtgcagg
    40561 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    40621 agcaactgaa acaacgcgcc attcattgcg gtcggctgga agagctagag attgacgggc
    40681 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgata gccattttta
    40741 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    40801 acggtatgtt gcatcttacc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    40861 agcgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    40921 tcttcgatca ggtggaaaat gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    40981 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagcg ccgcaacacg
    41041 ctgcttatct ggtacgtaat ctggatcttc ccggttttac ccccgcacag aaaaaactgc
    41101 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    41161 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    41221 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    41281 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    41341 ttgatcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    41401 taagtgccgg atgcgatgct gacacatctt ttccggcctt gattatttcc ctcgttttgc
    41461 tgccatcatc gctttcagat ttgccaaatg actttgccct ttcagcatcc gttcttcggc
    41521 gctgaccact ttgcgctcct gttcccaaat caaatcatcc tgcggcagct ccagcaggaa
    41581 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    41641 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    41701 attatcttca tcgatactgc tctggtgcgg cagaaatcct tcttccatac cgaccatgta
    41761 aacataagga aactccagtc cttttgacgc gtgcagggtc atcagttgca cctgatccag
    41821 ctcttcctcg ctctcgccgc gctccatcat gtcgcgcaga gtaaagcgcg tcaccacctg
    41881 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    41941 cagttggttg acgttcttca tgcgcatttc ggcagctttc gggctgggcg atgtttcgta
    42001 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    42061 ctccgccaga cgctggattt ctgccagcca gtgagtgaag cgggtcaatg cttcataacc
    42121 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    42181 gcgcgtcatc gcccattcac ctagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    42241 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    42301 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccgccag atattttgta
    42361 cgggatgcgg ttttgcatca ggaatttttc aaatacccgc gactgatggt tgccgcgata
    42421 aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga tcagctcgcc
    42481 ggtgacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta gctctgtgcc
    42541 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    42601 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    42661 cagcgccgga aaatcctggc tcagcagcac cagattctgc ggacgtgcgc cgcgccagga
    42721 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    42781 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcaggtagcg
    42841 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgttggcaa
    42901 taaaatcaga tcatcgaagt cgagaacgtt acaggctttc aggtgtgcat catacagccc
    42961 ataacaatgg gcaaaaatac ggtcccgctc gccctttgcc tctgccgccg cctgcgccgg
    43021 tgttttgaga tcgttcttcc agttggagat ggtcgaaatt agctgttgca ggagaacttt
    43081 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    43141 aaacagcgaa aagttagcct tcatccccag cgccgcatac tcgcgtttga tgatatccag
    43201 ccccagcgtg tggaaggtgg aaatcatcag cccacgcgcc tctttgcgcc ccagcgtctg
    43261 cccgacacgc tctttcatct cgcgtgccgc cttattggta aaggtcaccg ccgcgatatg
    43321 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga taacacgagt
    43381 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    43441 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    43501 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    43561 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc
    43621 agatcaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccgtcag
    43681 gcaaacgcgg cggtgatttg ggtgaattcc gccagggtca aatggttccg gcgttcgata
    43741 aagtggtttt ctcttgtccg gtactggagc caaccggccc gctgcatacc cagttcggtt
    43801 accacatcat taaggtgctg taccgtaact aacagcaagg ccttctccgg gagaaggcct
    43861 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    43921 cgcagtttct tacctacctg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    43981 agctgcgcgt tatctaccgc accttccgga atagctttac ccaggtcgcc cggttgcagc
    44041 tctgccataa acggtttcag cagtggcaca caagcgtaag agaacagata gttaccgtac
    44101 tcggcagtat cggagataac cacgttcatt tcgtacagac gcttacgggc gatggtgttg
    44161 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    44221 tcaaccatgg tttcaaacgc caactcaact cctgctttta ccatcgcgat catcagtacg
    44281 cctttatcga agtactcctg ttcgccgatt ttgccttcat actgcggcgc ggtttcgaat
    44341 gcggttttgc cagtctcttc acgccaggtc agcagtttct tatcgtcgtt ggcccagtca
    44401 gccatcatgc cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    44461 ggcgccatga tctctttcaa ttgctcagag agcgcgtaag cacgcagttt cgccgggtta
    44521 gaaagacggt ccatcatcag ggtgatgccg ccctgtttca gcgcttcggt gatggtttcc
    44581 caaccgaact gaatcagttt ttctgcgtat gccggatcgg tgccttcttc caccagcttg
    44641 tcgaagcaca gcagagaacc agcttgcaac ataccgcaca ggatggtttg ctcgcccatc
    44701 aggtcagatt tcacttccgc aacgaaagag gattccagaa cgcccgcacg gtgaccgccg
    44761 gttgcagccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    44821 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg tacttcggtg
    44881 ccagggcatt tcggcgcaac cattacgacg gtgatgtctt tacggatctg ctcacccact
    44941 tctacgatat tgaaaccatg agagtagccc agcgccgcgc cgtctttcat cagcggctga
    45001 acggaacgca ctacgtcaga gtgctgcttg tctggcgtca ggttaaccac cagatccgcc
    45061 tgcgggatca gttcttcgta agtgccaact ttgaagccgt tttcggtcgc tttacgccag
    45121 gatgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    45181 tcacgcatgt tcaggccctg gttcagaccc tgtgcaccac agccgacgat gactactttt
    45241 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    45301 cccagctgtg ccaactgctg gcgcagattc agtgtattga agtagttagc catggtgatt
    45361 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    45421 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    45481 gtcaacatta agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    45541 ggaaagccgc cattttggcc gcagcgcgcg ggcaatgcac gttagcccgt ccacgctctc
    45601 tcggcagatt cagcgcctgg aagacgatct cggtcagccg ctgtttgtgc gcgataaccg
    45661 cacggtaacg ctaaccgaag caggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    45721 gcagtatcaa cagctgcgcc acaccatcga tcagcaagga ccgtcgctct ctggcgaatt
    45781 acatatcttc tgctcggtga ccgctgccta cagccatctg ccgccgattc tcgatcgctt
    45841 ccgcgcggaa cacccgtcgg tggagattaa actcacgact ggtgatgcgg cagatgcgat
    45901 ggaaaaggtc gtcactggcg aagcggatct ggcgattgcc gggaaaccgg agactctgcc
    45961 cggctcagtg gcattttcga tgctggagaa tctggcagta gtgctgattg cccctgcgct
    46021 accctgcccg gtgcgtaacc aagtttcggc agataagccg gactggtcta cagtgccgtt
    46081 tattatggcc gatcagggac cggtgcgccg gcgcattgaa ctgtggtttc gacgcaacaa
    46141 aatcagcaac ccgatgattt acgctacggt cggcgggcat gaagcgatgg tgtcgatggt
    46201 agcgctcggc tgtggcgtag ccttgttgcc ggaagtggtg ctggaaaaca gcccggaacc
    46261 ggtgcgtaac cgcgtgatga ttttagagcg cagtgatgaa aaaacgccgt ttgaactggg
    46321 cgtgtgtgtg cagaaaaagc ggctacatga gccgctgatt gaggcgttct ggaagatttt
    46381 gccgaaccac taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    46441 atcgtagccc agttcattca gccgggtttc aaaatccggt tcatgatcgc caagctcgaa
    46501 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    46561 ccagtgcgta cccagcgtgt tgaggaagcg caacagcgcg cccggcgatt ccgggaattc
    46621 gaagctgtag aggcgttcct gcaacggatg cgacggacgc ccgccgacca tatagcgcac
    46681 atgcagcttc gccatttcgt cgtcggagag atccactacg ctgtagccgc catcgttgag
    46741 catctgcaaa atttctttgc gctcttcgag accacggctc aggcgcacac caacaaaaat
    46801 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgagc gcccgccaag
    46861 cagctggcag aacttcagga agctgccctt ctcttccggg atggttaccg ccagcaacgc
    46921 ttcacgctgt tcgcccagtt cgcaacgttc cgagacgtag cgcaggccgt gaaagttcac
    46981 gttagcacca gaaagaatat gcgccagccg ctcaccgcga atgttgtgct gggcgatata
    47041 ttttttcatt cccgccagtg ccagcgcgcc agagggttcc gccaccgcgc gcacatcttc
    47101 gaacaggtct ttcatcgccg cacatatggc atcgctatcg acggtgatga tgtcgtcgag
    47161 atactcctgg cataaacgga aggtttcgtc gccgatgcgt ttcaccgcaa caccttcggc
    47221 aaacagcccc acgcgtggca gatcaaccgg atgacctgca tccagcgccg ctttcaggca
    47281 ggcggagtct tccgcttcca cggcaatcac tttgatttgc ggcatcagtt gtttgatcag
    47341 taccgccacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    47401 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcgatca ccatcggatg
    47461 gtcgaacggc ggcacccagg tgaagccctg cagctgtgat agttcgatcg ctttcgcttt
    47521 tgcttcatca aagttcgcgc cgtgaagaag cacttcgccg ccgaagccgc gcaccgcgtc
    47581 gactttgata tcggcggtgg cggttggcat gacgatcagt gccttcacgc ctaaccgtgc
    47641 ggaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcggtgatca cgccgtgcgc
    47701 tttctgttct tccgtcaggc ccgccatcat ggcatatgcg ccgcgcagct taaagctatg
    47761 aactggctga cggtcttcgc gtttcaccag aatcacgtta tcgagacgcg acgagagttt
    47821 ttccattttt tgtagcggcg taacctgcgc cgcttcgtag accggcgcgc gcagcaccgc
    47881 tcttaaatat tcggcgcctt ccggggcgcc ggagagaggt tgtgagtctg ccatcattaa
    47941 ccccccagtt tcgatttatc acgcaccgcg cctttgtcgg cgctggttgc caggctggcg
    48001 taggcacgca gcgcaaagga aacctgacgt tcacgatttt tcggtgtcca ggctttgttg
    48061 ccacgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    48121 atgccacggt tcggaatgtc gatagcgatc aggtcaccat cttcaatcag gccaatgctg
    48181 ccgccacttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    48241 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaaactg
    48301 gttgggtaga gcatttcctg catccccgga ccgcctttcg ggccttcata gcgaattact
    48361 accacatctc cggcgacaac tttaccgccg agaatcgctt ctaccgcgtc gtcctggctt
    48421 tcgtagactt tcgccgggcc agtgaatttg aggatgctgt catcgacgcc tgcggtttta
    48481 acgatgcagc cgttttctgc gaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    48541 taggcgtgtt ccagcgagcg gatacagcca ttggagcgat cgtcgtccag cgtatcccag
    48601 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg ccgggccagc gcggaacata
    48661 ttttttaccg catcatcctg ggtcacgatg atgtcgtatt gttccagcgt ttgcggcaac
    48721 gtcaggccaa gtacattttt cacatcacgg ttcagtaacc ccgcacgatc cagctcgccg
    48781 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    48841 ggcgcaactt tacacagctg cggtactttg cgggagagct tatcgatatc actcatggtg
    48901 aagtcgattt ccgcttcctg cgccgccgcc agtaggtgaa gaacggtgtt ggtagagccg
    48961 cccatcgcga tatccagcgt catggcgttt tcaaacgccg ccttactggc gatgttacgc
    49021 ggcagtgcgc tttcatcatc ctgttcgtag taacgtttgg tcaattcaac aatgcgttta
    49081 ccagcattga ggaacagttg cttgcgatca gcgtgggttg ccagcagcga gccgttgccc
    49141 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcagt aaacatcccg
    49201 gagcaggaac cgcaggttgg gcaggcggat cgttcaacct gagcgctctg ggagtcagaa
    49261 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagcttaat gatctgatcg
    49321 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    49381 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    49441 atggcgtcgg cgcagtgggc gttgaccata tattcaacgg aatcagcgat cagttcgcga
    49501 gatggcagtg aataaagcat gcccccgtgg cccatagcga tcccatcatc caccgcaatg
    49561 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcaac cagtttaccg
    49621 agatcgcgta gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    49681 atcggcttac cgaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    49741 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    49801 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    49861 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    49921 ttgctgaatg cgtttggtaa ccgggccgca acggccttca ccaacctgaa taccatctac
    49981 actgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaggta
    50041 cagggattcg cgcgacagca cctgttcacg cacttcaatt cccagctctt tcgccagttt
    50101 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gagtgaacag
    50161 cacaccgtct ttcacttcaa acaggttttc tcctgcgcct tcagagatat aaccattcac
    50221 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccaacaggga
    50281 agagaggtag ttacctccag ctttcgctgc ggttgggatg gtgtttggtg cagcacggtt
    50341 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgcccc
    50401 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttacgcccat
    50461 gccaacatca ccgacgaaaa tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    50521 gatcacgtcg cgacaagctt ccatcagctc atcaatgctc tgcgaaaccg ggaaacgata
    50581 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    50641 tttgtgcgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    50701 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    50761 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    50821 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    50881 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    50941 catcgctggc ggcggccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    51001 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    51061 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    51121 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    51181 gtcgagtgcc gcttcaacct ggtctttatg ggtgatgtgt tggccaggga tgccgaaggc
    51241 gctggctaac atgaggaaat cggggttatc ggtaagggtt gtttcgctgt atcgttcctg
    51301 gaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    51361 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    51421 gccgtcaccg gagatacaga cgacggtatc gttcggtcgc gcgacttgtg cgccaaccgc
    51481 cgccggtaaa ccaaaaccca tggtgcctaa gccgctggag gtgatgaaat tttccgggcg
    51541 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    51601 gcaatccgca ggttttcgat ccgacagttg ttttaacaac aacggcgcgt agatagcgtc
    51661 accgggatgg tcgtaacgcc aggtatgttc atcacgcagc tgcgcgcagt attgctgcca
    51721 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccctgtaa
    51781 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    51841 gctggcgtgt ggtgcgaagg tgttcagttt gccagtcacc cggtcatcaa aacgtgcacc
    51901 cacggcgatc agtagatcgc actcctgcac tgcgaagttc gccgctttgg tgccgtgcat
    51961 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcaacgt
    52021 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccgaaa ctgcctgcgc
    52081 catgcccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctgacg
    52141 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacagtgg tgaaccacgg
    52201 ttccagatcg ccgctggcta actggatatc ttttggaata tcgaccaaaa ccggaccagg
    52261 acgaccagag ctggcgacgt caaacgcttc ggccatgata cgcggtaact cttccagtga
    52321 ttgcaccagg aaactgtgct tggtgcaggc taacgacaac cccagaatat ccacctcctg
    52381 aaatgcgtca gtgccgataa acggtgcgga tacctgaccg gtgatagcaa caacggggat
    52441 ggaatctaac agcgcgtccg caagcccggt tatcaggttg gttgcacctg gaccagacgt
    52501 ggcgatgcaa acgccagttt tgccggtagc acgggcataa ccgatagccg ccattgctgc
    52561 accttgctcg tgtcggcaca gcaagtgttc cacgccaccg tcatacaatg catcgtaaac
    52621 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    52681 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    52741 aattttgtgc tgttattcat tgtctgctcc tcagttatgt ttttaaggtc aaaaaaaacc
    52801 cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc
    52861 gtccaagtgc agccccgcac ggtgggataa taatcaccac catgctaatc acgaccaggc
    52921 taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc
    52981 taaagagtta ccatagattt tgcgaatagc acaagatatt ttttggccga tttttatgat
    53041 gtctttaata atctcaagag aaattgttgt tttttaaaaa gaaaatagtg agtgaaaaaa
    53101 attcaatttc agtatgaatg ttgttccctt cctcgtagtt catcctttgc ccgctgcatt
    53161 tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa
    53221 tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg
    53281 tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc ctgggagtaa
    53341 atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctaccaggc ttaacgatgg
    53401 tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg cgtgcgcagc gccattatca
    53461 atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc
    53521 caaaagaagg gggacgatat gatttgccta tcgctattgc gttactggcg gcctcagaac
    53581 agcttacagc caataagtta gatgaatatg aattagtcgg agaactggcg cttacaggcg
    53641 ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agctattaag tcgggcagaa
    53701 aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc
    53761 tgatagccga tcatctgcag gctgtctgcg cgtttctgga aggtaagcac gctctcgaac
    53821 acccgaaacc aactaatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg
    53881 gtcaggagca aggaaagcga ggactggaaa ttaccgccgc tggcgggcac aaccttttac
    53941 tgattggacc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggccttttgc
    54001 cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg
    54061 aatcagtaca aaaacaatgg cggcatcgcc cgttccgctc acctcatcac agtgcgtcgt
    54121 taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ctggcgcata
    54181 acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc
    54241 gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc
    54301 cagcccgttt ccagcttgtc gcggcaatga atcccagccc taccggacat tatcagggaa
    54361 accataaccg ctgcacacca gaacagacat tacgttatct caaccggctc tcggggccct
    54421 ttctcgaccg cttcgatctc tcactggaga tcccattacc gccccccggc attttgagta
    54481 aaacggtagt gccgggagaa aacagcacca ccgttaaaca acgtgtaatg gccgccagag
    54541 agcgccaatt taagcggcag aataaactga acgcctggct ggataatccg gaaatacgcc
    54601 aattctgcaa gcttgaaagc gaagatgcgc agtggctgga agaaacgctg atccatctgg
    54661 ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt gctgatattg
    54721 atcagtctga cattatcaca cgtcagcatt tgcaggaggc agttagctat cgtgcgattg
    54781 accgtttgct catccatctg caaaaactac tgacataaaa aaagggcatt tcgccctttt
    54841 tattaatcgt cagaatcggt gtagtcttca gcaccttcaa cctgcggttt accgccggaa
    54901 agggtgtgaa aacgttttgg acgcttgata cgcgtcatat acttggacca cacgcgttct
    54961 gcttctgtca ctggttcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg
    55021 gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc
    55081 agttgtgcct ctttgatggt gaagtcacca tgacgagaga atccacgtgg ataatgttta
    55141 ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct
    55201 ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac
    55261 atttaaatca taacgacaaa ttattttgcg gagagcactg tggatacgga attgttaaaa
    55321 actttcctgg aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg
    55381 acccaatcag ccgtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaatctt
    55441 ttcacccgcc acagaaacaa tatccgttta accgctgccg gagaaaagct tcttccttat
    55501 gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg aggtggcgca tacctcacga
    55561 cataatgagt tttctatcgg tgccagcgcc tcgctgtggg agtgtatgct taatcagtgg
    55621 ctgggacgtt tgtatcaaaa tcaggatgtc catacaggct tacagttcga agcgcgaatt
    55681 gcccaacggc agtctctggt aaaacagtta catgaacgcc agcttgatct tcttattaca
    55741 actgaagccc ccaaaatgga cgaattttgt agtcagttgc tggggtattt cactttagcg
    55801 ctttatacca gtgccccttc taaactaaag ggagatctta actatctgcg actagagtgg
    55861 gggccagatt ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt tcccattctg
    55921 acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg
    55981 ctacccgtca gttgggcacg taaaaaaggc ggcctgcata ccgttgtcga tagcacaaca
    56041 ctttcgcggc cgctttatgc catatggcta caaaatagcg ataaaaatgc gttgattcgc
    56101 gatctattga aaattaacgt gctggatgaa gtgtattaat ctgaatggct ggcaaggatg
    56161 ccggtagaag gatttacttc ggagagggtt atttcagata aaaaaaatcc ttagctttcg
    56221 ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg gttttggaga
    56281 ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga cgggactcga
    56341 acccgcgacc ccctgcgtga c
//
LOCUS       DAZFRE010000020        56029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFRE010000020 DAZFRE010000000
VERSION     DAZFRE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.020"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..542)
                     /gene="yjgL"
                     /locus_tag="ACHKUI_001453"
     CDS             complement(<1..542)
                     /gene="yjgL"
                     /locus_tag="ACHKUI_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO6597683.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEVLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASVSTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKILACYQREANK"
     gene            complement(665..1117)
                     /gene="tabA"
                     /locus_tag="ACHKUI_001454"
     CDS             complement(665..1117)
                     /gene="tabA"
                     /locus_tag="ACHKUI_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6597684.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1262..1855)
                     /gene="bdcR"
                     /locus_tag="ACHKUI_001455"
     CDS             complement(1262..1855)
                     /gene="bdcR"
                     /locus_tag="ACHKUI_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6597685.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            1926..2639
                     /gene="bdcA"
                     /locus_tag="ACHKUI_001456"
     CDS             1926..2639
                     /gene="bdcA"
                     /locus_tag="ACHKUI_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6597686.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2770..3165
                     /gene="yjgH"
                     /locus_tag="ACHKUI_001457"
     CDS             2770..3165
                     /gene="yjgH"
                     /locus_tag="ACHKUI_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6597687.1"
                     /translation="MVERTAVFLAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3447..3581
                     /gene="pyrL"
                     /locus_tag="ACHKUI_001458"
     CDS             3447..3581
                     /gene="pyrL"
                     /locus_tag="ACHKUI_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6597688.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3585..4520
                     /gene="pyrB"
                     /locus_tag="ACHKUI_001459"
     CDS             3585..4520
                     /gene="pyrB"
                     /locus_tag="ACHKUI_001459"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6597689.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4533..4994
                     /gene="pyrI"
                     /locus_tag="ACHKUI_001460"
     CDS             4533..4994
                     /gene="pyrI"
                     /locus_tag="ACHKUI_001460"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6597690.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5067..5453
                     /gene="ridA"
                     /locus_tag="ACHKUI_001461"
     CDS             5067..5453
                     /gene="ridA"
                     /locus_tag="ACHKUI_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6597691.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5659..8355)
                     /gene="mgtA"
                     /locus_tag="ACHKUI_001462"
     CDS             complement(5659..8355)
                     /gene="mgtA"
                     /locus_tag="ACHKUI_001462"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6597692.1"
                     /translation="MFKEIFTRLTRHLPSRLVHRDPLPGVQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLHQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVATKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8496..8549)
                     /gene="mgtL"
                     /locus_tag="ACHKUI_001463"
     CDS             complement(8496..8549)
                     /gene="mgtL"
                     /locus_tag="ACHKUI_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6597693.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8731..9681
                     /gene="treR"
                     /locus_tag="ACHKUI_001464"
                     /pseudo
     CDS             8731..9681
                     /gene="treR"
                     /locus_tag="ACHKUI_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            9800..11221
                     /gene="treB"
                     /locus_tag="ACHKUI_001465"
     CDS             9800..11221
                     /gene="treB"
                     /locus_tag="ACHKUI_001465"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6597694.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11271..12926
                     /gene="treC"
                     /locus_tag="ACHKUI_001466"
     CDS             11271..12926
                     /gene="treC"
                     /locus_tag="ACHKUI_001466"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6597695.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGKMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13320..15458
                     /gene="nrdD"
                     /locus_tag="ACHKUI_001467"
     CDS             13320..15458
                     /gene="nrdD"
                     /locus_tag="ACHKUI_001467"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6597696.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15616..16080
                     /gene="nrdG"
                     /locus_tag="ACHKUI_001468"
     CDS             15616..16080
                     /gene="nrdG"
                     /locus_tag="ACHKUI_001468"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6597697.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16125..16511)
                     /gene="cybC"
                     /locus_tag="ACHKUI_001469"
     CDS             complement(16125..16511)
                     /gene="cybC"
                     /locus_tag="ACHKUI_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6597698.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16567..17919)
                     /gene="pmbA"
                     /locus_tag="ACHKUI_001470"
     CDS             complement(16567..17919)
                     /gene="pmbA"
                     /locus_tag="ACHKUI_001470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6597699.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18013..18564
                     /gene="yjgA"
                     /locus_tag="ACHKUI_001471"
     CDS             18013..18564
                     /gene="yjgA"
                     /locus_tag="ACHKUI_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6597700.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRKLAENEG"
     gene            complement(18720..20093)
                     /gene="mpl"
                     /locus_tag="ACHKUI_001472"
     CDS             complement(18720..20093)
                     /gene="mpl"
                     /locus_tag="ACHKUI_001472"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6597701.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPVDAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20269..21267
                     /gene="fbp"
                     /locus_tag="ACHKUI_001473"
     CDS             20269..21267
                     /gene="fbp"
                     /locus_tag="ACHKUI_001473"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6597702.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21300..22295)
                     /gene="yjfF"
                     /locus_tag="ACHKUI_001474"
     CDS             complement(21300..22295)
                     /gene="yjfF"
                     /locus_tag="ACHKUI_001474"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6597703.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22282..23304)
                     /gene="ytfT"
                     /locus_tag="ACHKUI_001475"
     CDS             complement(22282..23304)
                     /gene="ytfT"
                     /locus_tag="ACHKUI_001475"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6597704.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAELLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23318..24820)
                     /gene="ytfR"
                     /locus_tag="ACHKUI_001476"
     CDS             complement(23318..24820)
                     /gene="ytfR"
                     /locus_tag="ACHKUI_001476"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6597705.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24960..25916)
                     /gene="ytfQ"
                     /locus_tag="ACHKUI_001477"
     CDS             complement(24960..25916)
                     /gene="ytfQ"
                     /locus_tag="ACHKUI_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6597706.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26226..26756
                     /gene="ppa"
                     /locus_tag="ACHKUI_001478"
     CDS             26226..26756
                     /gene="ppa"
                     /locus_tag="ACHKUI_001478"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6597707.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27136..27477)
                     /gene="ytfP"
                     /locus_tag="ACHKUI_001479"
     CDS             complement(27136..27477)
                     /gene="ytfP"
                     /locus_tag="ACHKUI_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6597708.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27480..31259)
                     /gene="tamB"
                     /locus_tag="ACHKUI_001480"
     CDS             complement(27480..31259)
                     /gene="tamB"
                     /locus_tag="ACHKUI_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6597709.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLDCLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGSVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     LLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVLWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31256..32989)
                     /gene="tamA"
                     /locus_tag="ACHKUI_001481"
     CDS             complement(31256..32989)
                     /gene="tamA"
                     /locus_tag="ACHKUI_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6597710.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33195..33833
                     /gene="msrA"
                     /locus_tag="ACHKUI_001482"
     CDS             33195..33833
                     /gene="msrA"
                     /locus_tag="ACHKUI_001482"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6597711.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34156..35499
                     /gene="paeA"
                     /locus_tag="ACHKUI_001483"
     CDS             34156..35499
                     /gene="paeA"
                     /locus_tag="ACHKUI_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6597712.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35561..35767)
                     /gene="ytfK"
                     /locus_tag="ACHKUI_001484"
     CDS             complement(35561..35767)
                     /gene="ytfK"
                     /locus_tag="ACHKUI_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6597713.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36092..36649
                     /gene="ytfJ"
                     /locus_tag="ACHKUI_001485"
     CDS             36092..36649
                     /gene="ytfJ"
                     /locus_tag="ACHKUI_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6597714.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36639..37379)
                     /gene="cysQ"
                     /locus_tag="ACHKUI_001486"
     CDS             complement(36639..37379)
                     /gene="cysQ"
                     /locus_tag="ACHKUI_001486"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6597715.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37569..39512
                     /gene="cpdB"
                     /locus_tag="ACHKUI_001487"
                     /pseudo
     CDS             37569..39512
                     /gene="cpdB"
                     /locus_tag="ACHKUI_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(39635..40015)
                     /gene="ytfH"
                     /locus_tag="ACHKUI_001488"
     CDS             complement(39635..40015)
                     /gene="ytfH"
                     /locus_tag="ACHKUI_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6597716.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHAEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40104..40964
                     /gene="qorB"
                     /locus_tag="ACHKUI_001489"
     CDS             40104..40964
                     /gene="qorB"
                     /locus_tag="ACHKUI_001489"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6597717.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            41072..42037
                     /gene="ytfF"
                     /locus_tag="ACHKUI_001490"
     CDS             41072..42037
                     /gene="ytfF"
                     /locus_tag="ACHKUI_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6597718.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42145..42807
                     /gene="ytfE"
                     /locus_tag="ACHKUI_001491"
     CDS             42145..42807
                     /gene="ytfE"
                     /locus_tag="ACHKUI_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6597719.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(42852..44264)
                     /gene="cycA"
                     /locus_tag="ACHKUI_001492"
                     /gene_synonym="dagA"
     CDS             complement(42852..44264)
                     /gene="cycA"
                     /locus_tag="ACHKUI_001492"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6597720.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44573..45193)
                     /gene="fklB"
                     /locus_tag="ACHKUI_001493"
     CDS             complement(44573..45193)
                     /gene="fklB"
                     /locus_tag="ACHKUI_001493"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6597721.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45412..46050
                     /gene="ytfB"
                     /locus_tag="ACHKUI_001494"
     CDS             45412..46050
                     /gene="ytfB"
                     /locus_tag="ACHKUI_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6597722.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTSQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46034..46141)
                     /locus_tag="ACHKUI_001495"
                     /pseudo
     CDS             complement(46034..46141)
                     /locus_tag="ACHKUI_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(46185..47382)
                     /gene="insQ"
                     /locus_tag="ACHKUI_001496"
                     /pseudo
     CDS             complement(46185..47382)
                     /gene="insQ"
                     /locus_tag="ACHKUI_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            47390..47815
                     /gene="tnpA"
                     /locus_tag="ACHKUI_001497"
                     /pseudo
     CDS             47390..47815
                     /gene="tnpA"
                     /locus_tag="ACHKUI_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(47879..48172)
                     /locus_tag="ACHKUI_001498"
                     /pseudo
     CDS             complement(47879..48172)
                     /locus_tag="ACHKUI_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            48439..49233
                     /gene="yjfZ"
                     /locus_tag="ACHKUI_001499"
     CDS             48439..49233
                     /gene="yjfZ"
                     /locus_tag="ACHKUI_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6597723.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATRCNAYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYTGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(49304..49753)
                     /gene="rplI"
                     /locus_tag="ACHKUI_001500"
     CDS             complement(49304..49753)
                     /gene="rplI"
                     /locus_tag="ACHKUI_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6597724.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(49795..50022)
                     /gene="rpsR"
                     /locus_tag="ACHKUI_001501"
     CDS             complement(49795..50022)
                     /gene="rpsR"
                     /locus_tag="ACHKUI_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6597725.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(50027..50341)
                     /gene="priB"
                     /locus_tag="ACHKUI_001502"
     CDS             complement(50027..50341)
                     /gene="priB"
                     /locus_tag="ACHKUI_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6597726.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(50348..50743)
                     /gene="rpsF"
                     /locus_tag="ACHKUI_001503"
     CDS             complement(50348..50743)
                     /gene="rpsF"
                     /locus_tag="ACHKUI_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6597727.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            51070..51345
                     /gene="yjfY"
                     /locus_tag="ACHKUI_001504"
     CDS             51070..51345
                     /gene="yjfY"
                     /locus_tag="ACHKUI_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6597728.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(51474..52160)
                     /gene="ulaF"
                     /locus_tag="ACHKUI_001505"
     CDS             complement(51474..52160)
                     /gene="ulaF"
                     /locus_tag="ACHKUI_001505"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6597729.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(52160..53014)
                     /gene="ulaE"
                     /locus_tag="ACHKUI_001506"
     CDS             complement(52160..53014)
                     /gene="ulaE"
                     /locus_tag="ACHKUI_001506"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6597730.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(53024..53674)
                     /gene="ulaD"
                     /locus_tag="ACHKUI_001507"
     CDS             complement(53024..53674)
                     /gene="ulaD"
                     /locus_tag="ACHKUI_001507"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6597731.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(53688..54152)
                     /gene="ulaC"
                     /locus_tag="ACHKUI_001508"
     CDS             complement(53688..54152)
                     /gene="ulaC"
                     /locus_tag="ACHKUI_001508"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6597732.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(54162..54467)
                     /gene="ulaB"
                     /locus_tag="ACHKUI_001509"
     CDS             complement(54162..54467)
                     /gene="ulaB"
                     /locus_tag="ACHKUI_001509"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6597733.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(54483..55880)
                     /gene="ulaA"
                     /locus_tag="ACHKUI_001510"
     CDS             complement(54483..55880)
                     /gene="ulaA"
                     /locus_tag="ACHKUI_001510"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6597734.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMTTIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLEIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
BASE COUNT        13697 a        14963 c        13939 g        13430 t
ORIGIN      
        1 tccttgttag cttcgcgttg gtaacacgcc agaatcttgt tagtaatcag gtgtaatgaa
       61 ctatcatctt ttctttcaaa agacttccct tcataacgat tagtcgaacc atcagattgt
      121 ttaaatataa tgactgtatg tccttcacga atccctttag tagaaactga ggcaagtgta
      181 atttctatct tcaccttaga taaatccgct cgctcccctt caggattatt catgtaaaac
      241 ttcatttttt cattgccatc agtatcttta gaataagatg aggactcaat atgagccatc
      301 atatttctac ccgcttcgtt ggagcgtttg ctaaaatcca cataacccaa ggtaaatatc
      361 tttgccaaaa actccaaaaa tgctctagga gaaagaatat tgcttctgct attctgcccc
      421 ccggcaattc gggcaaaatc attcattccc acataccagg tatctaagac ttcatttttt
      481 tgtttaaaat tgtcggctaa tgtaatgtgt tgagaataat tcaaatctga tattttgctc
      541 atgacgaact ccttgagtta ataagaacgt ctaagcatta caaattttac tcacaaaaaa
      601 atatcaaaaa acgctctttt cacaaactcg ccgtaaacct ggagttattt acggcgaaaa
      661 gtcatcacgc catcagcatc ttcactactg ctttgcgaac ccgggctggc gcgcccactg
      721 cgcacagcgg tttatgcact tcccccggat aaaacacaac aaaatcacct tcattaagga
      781 taactgtttt ctcatcaacg ccttccggca aaaatgcgat gtctttatca gctaaccagt
      841 cggtatccgg ggtgcctgca ggttgcgtgc tgaaggtcat gccttcctga ccttttaaca
      901 caatctgaat gtcgagatag cgggcatggt actccgcacg gcgagcttcg tgcggctcgg
      961 tcatatcttc cgagataaga taaaacagac gattgccttc gatatcgtgc ttgccctttg
     1021 gcgtttctgc cgtaacgtgt gctttgatat gctcaatcgc ctggcgtaac tcctggggta
     1081 gccacggctg aagattatga atatttccga tgatcatctt ttccactcct taattaaaac
     1141 aacgtttcat ttctatcgtt atacgaaaag atgatcaatt ccgctagcct taacgcgtga
     1201 attattgtct tatcacaact tatcggcagg cgcgttcccg cctttcgggt caaggcccgc
     1261 atcactcctt gagaatagtt tttatcgctt cccccgccag tgcagctgtt gcacagagtt
     1321 gttcgatgga gtgcccctcc cgtgccttcg cagaaagccc tgacatcacg gtactcataa
     1381 aatcagtcac acattgtgcg ctttgtggat gccgcctggc gatatagtca taaatggccg
     1441 tttctgcggc gtgataatat tgaacggcaa tatcacgcgc ttgtggatca tgactatgaa
     1501 tgccttcaag aaccatacag ccagcgcagc cgccgttttg gctatatctg cgcgccgctt
     1561 cttttaatac ctcagccagg cactccccta ccggacgatc atcacgaaga atatcggcaa
     1621 gcggaatagc ttccgtaccg acatattcat tgagtacacg gctaaataac ccagccttat
     1681 tgccaaaagc cgcgtagagg ctcggggggt taataccaag ataatcagta acttcagcaa
     1741 cactgacagc atcgaaacct ttttggtgaa aaagcacttt tgccgcagag actgcctgct
     1801 caggagcgaa acgtctggga cgacctggaa cgcgagattg ttttttagtg accattacaa
     1861 aacttattga cagaaagtta aaacagtttt gtaatgcatg ttacataata aatcaaggag
     1921 tccttatggg cgcttttaca ggtaagacag ttctcatcct cggtggtagc cgtggaatcg
     1981 gtgccgctat cgtacgtcgt ttcgtcaccg atggggccaa tgtacgattc acctatgcgg
     2041 ggtcgaaaga tgccgctgaa cgcctggcac aagagactgg agcgacagca gtattcacag
     2101 atagtgctga cagagacgct gtcattgatg tcgttcgtaa gagcggcgca ttggatatcc
     2161 tggtggtaaa tgcaggtatt ggcgtctttg gcgatgccct ggaattgaat gccgacgata
     2221 ttgatcgcct tttcaaaatc aatattcatg ctccttatca tgcctctgtt gaagccgccc
     2281 ggcagatgcc cgaaggcggg cgcatcttaa tcatcggctc cgtgaatggc gatcgtatgc
     2341 ctgttgcagg catggctgct tatgccgcca gtaaatctgc cctgcaaggc atggcgcgcg
     2401 ggctggcccg tgatttcgga ccgcgtggga tcaccatcaa cgtcgtccag ccagggccaa
     2461 ttgataccga cgctaatccc gccaacgggc caatgcgcga tatgttgcat agttttatgg
     2521 ctatcaaaag acatgggcaa ccggaagagg tcgctggtat ggtcgcatgg ttagcagggc
     2581 cagaagccag ttttgttacc ggcgcgatgc ataccattga tggcgcgttt ggcgcataac
     2641 cgactacgct caattaagcc cagccatttc ccatgatgtc tgggttttgt ttactcacgt
     2701 cgtccgctaa aagcggctcc tggtaaatat aaatcttctg ttcatgtcgt aaatcttgag
     2761 gataaccaaa tggtagaaag aaccgctgtt ttccttgctg gccgacattc actgtatgct
     2821 gaacatcgtt attctgcggc tattcgttcc ggcgatttgc tgtttgtttc cgggcaagta
     2881 ggaagtcgag aggacggaac accagaaccc gattttcagc aacaagtcag actggcattt
     2941 gataatttgc atgcgaccct ggcagctgcg ggatgcactt ttgacgatat cattgatgtt
     3001 acgagctttc ataccgatcc agaaaaacaa tttgaagaca tcatgacggt gaaaaatgaa
     3061 atatttagcg cgccacctta tccaacctgg acggcggtgg gcgttacatg gctggcaggc
     3121 tttgattttg aaattaaagt gatagcgcgc atccctgagc tgtaagcaat agtgttagcc
     3181 gttcgttttc acactccgcc ctataagtcg gatgaatgga ataaaatgca tatctgattg
     3241 cgtgaaagtg aaaaagaaaa aagcaggaaa tgtctgcaat tattgatacc gaacgacagt
     3301 tccccctcca gaatcacagt aaataaaaat gcatatattt tgactttaaa ttcaaataaa
     3361 ccgtttgcgc tgacaaaata ttgcatcaaa tgcttgcacc gcttctgacg atgagtataa
     3421 tgccggacaa tttgccggga ggatgtatgg ttcagtgtgt tcgacatttt gtcttaccgc
     3481 gtctgaaaaa agacgctggc ctgccgtttt tcttcccgtt gatcacccat tcccagcccc
     3541 tcaatcgagg ggcttttttt tgcccaggcg tcaggagata aaagatggct aatccgctat
     3601 atcagaaaca tatcatttcc ataaacgacc ttagtcgcga tgaccttaat ctggtgctgg
     3661 caacagcggc gaaactgaaa gcaaacccgc aaccagagct gttgaagcac aaagtcattg
     3721 ccagctgttt cttcgaagcc tctacccgta cccgcctctc tttcgaaact tccatgcacc
     3781 gcctgggtgc cagcgtggtg ggcttctccg acagcgccaa tacatcactg ggtaaaaagg
     3841 gcgaaacgct ggccgatacc atttcggtta tcagcactta cgtcgatgcg atagtgatgc
     3901 gtcatccgca ggaaggtgcg gcgcgcctgg ccaccgagtt ttccggcaat gtaccggtac
     3961 tgaatgccgg tgatggctcc aaccaacatc cgacgcaaac cttgctggac ttattcacta
     4021 ttcaggaaac ccaggggcgt ctggacaatc tccacgtcgc aatggttggt gacctgaaat
     4081 atggccgcac cgttcactcc ctgactcagg cgttagcgaa gttcgacggc aaccgttttt
     4141 acttcatcgc gccggacgcg ctggcaatgc cgcaatacat tctggatatg ctcgatgaaa
     4201 aagggatcgc atggagtctg cacagctcta ttgaagaagt gatggcggaa gttgacatcc
     4261 tgtacatgac ccgcgtgcaa aaagagcgtc tggacccgtc cgagtacgcc aacgtgaaag
     4321 cgcagtttgt tcttcgcgcc agcgatctcc acaacgccaa agccaatatg aaagtactgc
     4381 atccgctgcc gcgtgttgat gagattgcga cggatgttga taaaacgcca cacgcctggt
     4441 acttccagca ggcaggcaac gggattttcg ctcgccaggc gttactggca ctggttctga
     4501 atcgcgatct ggtactgtaa ggggaaatag agatgacaca cgataataaa ttgcaggttg
     4561 aagctattaa acgcggcacg gtaattgacc atatccccgc ccagatcggt tttaagctgt
     4621 tgagtctgtt caagctgacc gaaacggatc agcgcatcac catcggtctg aacttgcctt
     4681 ctggcgagat gggccgcaaa gatctgatca aaatcgaaaa tacctttttg agtgaagacc
     4741 aggtagatca actggcattg tatgcgccgc aagccacggt taaccgtatc gacaactatg
     4801 aagtggtggg taaatcgcgc ccaagtctgc cggagcgcat cgacaatgtg ctggtctgcc
     4861 cgaacagcaa ctgtatcagc catgccgaac cggtttcttc cagctttgcc gtgcgaaaac
     4921 gcgccaatga catcgcgctc aaatgcaaat actgtgaaaa agagttttcc cataatgtgg
     4981 tgctggccaa ttaattgcgg ttggtaataa aagtctggct ccctataatg agccagactt
     5041 tttaccgctg taataaagga gaaatcatga gcaaaactat cgcgacggaa aatgcaccgg
     5101 cagctatcgg cccttacgta cagggcgttg atctgggcaa tatgatcatc acctccggtc
     5161 agatcccggt aaatccgaaa acgggcgaag taccggcaga cgtcgctgca caggcacgtc
     5221 agtcgctgga taacgtaaaa gcgatcgtcg aagccgctgg tctgaaagtg ggcgacatcg
     5281 ttaaaactac cgtgtttgta aaagatctga acgacttcgc aaccgtaaac gccacttacg
     5341 aagccttctt caccgaacac aacgccacct tcccggcacg ttcttgcgtt gaagttgccc
     5401 gtctgccgaa agacgtgaag attgagatcg aagcgatcgc tgttcgtcgc taatcttgat
     5461 ggaaatccgg gctatcatgc ccggattaag tctgatgaca aacgcaaaat cgcctgatgc
     5521 gctacgctta tcaggcctac gtgattcctg caatttattg aatttgttgg ccggataagg
     5581 catttacgcc gcatccggca tgaacaaaac tcactttgtc tgcaatctga atccgggtca
     5641 ccatgcccag ttgattcttt attgccagcc gtaacgacgg ctatagaacc ctttcaccaa
     5701 ctgggttaat gtcatatacc ctgccagaat cgcaaccagc cacgggaaat agcttaacgg
     5761 cagcgcctgc agctgcagat agctggccag cggtgaaaac ggcaatgcga taccgacaat
     5821 catcacgatc acggtcatga tcattaacgg ccacgatgca cagctctgaa taaacggcac
     5881 acggcgggtg cggatcatat gcacaatcag cgtttgcgac agtaagccca ccacaaacca
     5941 tcccgactgg aagagcgttt gcgtttccgg cgtgttggca tggaataccc accacatcag
     6001 gcaaaacgtc agaatatcga agatcgagct gatcggtccg aagaagatca taaagcgccc
     6061 cagatccgcc ggattccaac gctgcggctt ttgaatttgc tcgtcgtcga cgttatcaaa
     6121 cgggatcgcc acctgtgaca catcgtacag caggttctga ataagcaagt gtaacggcaa
     6181 catcggcagg aagggcaaga aagcactcgc taccagcacg ctgaacacat taccgaagtt
     6241 agagctcgcc gtcattttga tgtatttcag catgttggcg aaagtgcgtc gtccctcaat
     6301 aaccccctct tccagcacca tcaggctttt ttccagcagg atgatatcag ccgcttcacg
     6361 ggcaatatct accgcgccgt ccacagaaat gccgatatcc gcagcgcgta aggcgggcgc
     6421 atcattaata ccatcgccca taaagccaac cacatgccct tcgcgcttca gtaaggtcac
     6481 aatgcgttct ttatgcatcg gcgtcaggcg ggcaaacagc gtggtgcgct gtgcaagatt
     6541 tgccagttcg tcgtcagata gcgtttcaat atcactacca atgaccacct ctcccgcgtc
     6601 cagccccact tcatggcaca ctttcgttgc aactaactca ctgtcgccag taaggatttt
     6661 tacggtaatt ccactcgctt ttaatgcctt cagtgccgga gcggttgtct cttttggcgg
     6721 atcaagaaaa gcaatatatc cttcgaggat caggtcggat tcatccgccc gctggtaatc
     6781 tccttcacgc gctggcaggt atttcgtcgc caccgcaacc acgcgcagcc cctgacgatt
     6841 cagcgtatca gtaacccgct taatcttacg cagcatgatg tcatcgagcg gcacaatctc
     6901 gccattgtga cgcacctgcg aacacacatt gaggatttcc tgcaatgcac ctttgcaaac
     6961 cagctgatgg tgctcggtat tttctgccac taccaccgac atccggcgac gctcgaaatc
     7021 aaacggaatc tcatcgattt tttgccaacg actggccagc gagcgcgctg actcttcatc
     7081 cgtaccttcg agcaccgctg tatcgagcag gtttttaagt ccggtctgat aatgactgtt
     7141 caaccacgcg ctatgcagca cgcgttcgct ggttttaccg gagatatcgg tatgattctc
     7201 cagcacaatt ttatcctgtg tcagggtgcc ggttttatca gtgcacagaa tatccattgc
     7261 gccaaagttc tgaatagcat ccagatgttt gacgatcact ttctgtttcg acagctttac
     7321 tgcgccgcgc gccagcgtcg aggtaacaat catcggcaac atttccggcg ttaggcctac
     7381 cgcgaccgaa agcgcaaaca gcgccgcttc ccaccagtcg cctttggtgt agccattgat
     7441 taacagcacc accggtgcca tcaccagcat aaagcgaatc agcagcatac tgacgcggct
     7501 gatcccttgc tgaaaggcat tcggctcgct ttcctgctca ctaacacgcc ccgccagttg
     7561 accaaaccag gtattggcac ctgtagcaat caccattgct tgtgccgtgc cgctcaccac
     7621 ggtggtgccc ataaaacaca gcgtgtcgca ctccagcgga ttgctgtgct ccggctggcg
     7681 agtggtagcg gctttttcca cgggcagaga ctcaccggtt aacgacgcct gagcgacgaa
     7741 cagatcccgc gcctgcaaga tacgtaaatc tgccgggatc atatctcccg ccgccagttt
     7801 tataatatcg ccgggcacca gctggtcgat cgggatctcc agccagccat tttcgccttt
     7861 gtcgttaatt acgcgcagca ccgtcgcggt attgctgacc atcgctttca gggcatctgc
     7921 cgctttagtg gaacgtgctt cctgaataaa gttcagcaac gttgaaatag cgaccattag
     7981 cgcgataacg cccgcggcaa ataaatcttc cgtggcgtaa gaaatagcgc cgagaatggt
     8041 gagtaaaata ttaaaggggt tgcgatagca gacccataaa tgtacccacc acggcgacgg
     8101 ttgttgtgcg ggtaatttat tttcaccatg ttgttcgcgg gcagattcca cttccgcctg
     8161 atgtaaccct tccggatggg tgtcgaacgt tttccacaat tcttcttcgg gcatcaccgc
     8221 cattttcagg caatgcgcac ttaaggacgg cgggaccacc gtattcactg tctgctgcac
     8281 gccaggcaat ggatcacgat gaaccagacg ggaaggtaaa tggcgagtga gccgggtaaa
     8341 aatttcttta aacataagga gtccctccgc actgtctgaa acagtacaga aatgtcttca
     8401 ggcgtggcga agccttacct gaaggtaata aagctgagca gggacgttaa aacgtcacgg
     8461 ccttacgcat ccggtaacgc cgtgaaaaga cgggcttacc ggaaaagttt taatctccgt
     8521 cgagggagag gcgtgggatc aggttccata taacctccgg taagtgaaaa aagcgccgca
     8581 aataataagg agaatccaat aatattcgta gcatttatgg cgatataata cctgcaggca
     8641 actaaaccaa cgataaacca gactttaccc attgctgaat gcacgggtaa agttagcctc
     8701 aaataattaa acaacacgtt acgggacaac aggatgcaaa atcggctgac catcaaagac
     8761 atcgcgcgct taagcggcgt ggggaaatct acggtttccc gggtgctgaa taacgaaagc
     8821 ggcgtgagcc agcgcacgcg cgagcgtgtt gaagcagtga tgaatcagca tggattttcc
     8881 ccttcccgtt ctgcacgcgc tatgcgtggg caaagcgata aagtggtcgc catcattgtc
     8941 acccgtctgg attcgttgtc agaaaatctc gccgttcaaa ccatgctgcc agcgttctat
     9001 gaacaaggtt acgatccaat catgatggaa agtcagtttt ccccgcaatt agttgccgaa
     9061 catttggggg tgctgaaacg gcgtaatatc gacggcgtag tgctgttcgg ttttaccggc
     9121 ataacagaag aaatgttagc ccactggcag tcatcgctgg ttctgctggc gcgtgacgca
     9181 aaaggctttg cttcggtctg ttatgacgac gaaggggcaa tcaagatcct gatgcaacgg
     9241 ctgtatgacc aggggcatcg caatatcagt tatctcggcg tgccgcatag tgacgtgacg
     9301 accgggaagc gacgtcacga agcctacctg gcgttctgca aagcgcataa actgcatccc
     9361 gttgccgccc tgccaggcct tgctatgaag caaggctatg agaacgtagc aaaagtgatt
     9421 acgcctgaaa ctaccgcctt actgtgcgca accgacacgc tggcacttgg cgcaagtaaa
     9481 tacctgcaag agcaacgcat cgacaccttg caactggcga gcgtcggtaa tacgccatta
     9541 atgaaattcc ttcatccgga gatcgtcacc gtagatcctg gttacgccga agctggacgc
     9601 caggcggcct gccagttgat cgcgcaggta accggacgca gcgaaccgca acaaatcatc
     9661 atccccgcca ccctgtcctg atcgtttcct gaacgataaa ttgtgatctt cgctgcgttt
     9721 cgggaacgtt cccgttttta aatttttccg cgcaatatat tctgcagcca accaaaaatg
     9781 tcatctgcca tggggcttta tgatgagcaa aataaaccaa acggatatcg atcggttgat
     9841 tgaactggtc ggcgggcgcg gcaatattgc gacggtgagc cactgtatta ctcgcctgcg
     9901 ctttgtcctc aaccaaccgg ccaatgccag accgaaagaa attgagcaac tccccatggt
     9961 gaaaggctgt ttcaccaatg ccgggcaatt tcaggtggtg attggcacca acgtgggtga
    10021 ttactatcaa gcactgatag cgtcaaccgg acaggcgcag gttgataaag agcaggtaaa
    10081 aaaagccgcc cggcagaata tgaaatggca tgagcagttg atctctcatt tcgcggagat
    10141 cttcttcccg ttgctgcccg cgttgattag cggcggtttg atcctcggtt ttcgcaatgt
    10201 gatcggcgat ttgcccatga gcaacggtca aacgctggcg caaatgtacc cttccctgca
    10261 aacgatctac gattttctgt ggttgatcgg tgaagcgatc ttcttctacc tgccggtcgg
    10321 gatttgctgg tcggcggtga aaaaaatggg cggcacgccg atccttggta tcgtacttgg
    10381 cgtgacactg gtttcccccc agctgatgaa cgcttatctg ctcgggcagc agctgccgga
    10441 agtgtgggac tttggcatgt tcagcatcgc caaagtgggc tatcaggcgc aggtgatccc
    10501 ggcactgtta gccgggctgg cgctgggcgt tattgaaact cgccttaaac gcatcgtgcc
    10561 agattacctc tatctggtgg tggtgcccgt ctgttcgctg atcctcgcgg tgttcctcgc
    10621 ccatgcgctg attggtccgt ttggtcgcat gattggcgat ggcgttgcct ttgcggtacg
    10681 tcacctgatg accggcagct ttgctccgat tggcgcggca ttgtttggct tcctgtacgc
    10741 cccgctggtg atcaccgggg tacaccagac cacgcttgct attgatttgc agatgattca
    10801 aagcatgggt ggtacgccag tgtggccgct gattgcgctg tcgaatatcg ctcagggctc
    10861 cgccgtgata ggcattatca tttccagccg caagcacaat gaacgcgaga tctccgttcc
    10921 tgccgctatc tccgcctggc ttggggtcac tgagcctgca atgtacggca tcaacctgaa
    10981 atatcgcttc ccgatgctgt gcgcgatgat tggttctggt ctggcaggat tgctatgtgg
    11041 cctgaacggc gttatggcga atggcatcgg cgtaggcggc ctgccgggaa ttctctcgat
    11101 tcaaccgagc tactggcagg tgtttgcgct ggcaatggct atcgccatca tcatcccgat
    11161 tgtactcacc tcgtttatct atcagcggaa ataccgcctg ggcacgctgg acattgttta
    11221 attttcttcg gggcgcaatt gcgccccctc gcatttgcag gaataacgta atgactaatc
    11281 ttccccactg gtggcaaaac ggcgttatct accagattta tccaaagagt tttcaggaca
    11341 ccacgggtag cggcaccggc gatttacgtg gcgttatcca acgcctggac tatctgcata
    11401 aactgggcgt tgatgccatc tggctaaccc ccttttatgt ctctccgcag gtcgataacg
    11461 gttacgatgt agcgaactat acggcgattg atcccaccta cggcacgctg gacgattttg
    11521 acgaactggt gacgcaggca aaatcgcgtg ggattcgtat cattctcgat atggtgttta
    11581 accatacctc tacccaacat gcctggtttc gcgaggcgct gaacaaagaa agcccttacc
    11641 gccagtttta tatctggcgc gatggcgaac cagaaacgcc accgaacaac tggcgttcaa
    11701 aatttggcgg tagtgcgtgg cgctggcatg cggaaagcga acagtactat ttgcatctct
    11761 ttgcaccaga acaggcggat ctcaactggg agaatccagc ggtacgcgca gagctgaaaa
    11821 aagtctgtga gttctgggcc gatcgtgggg tcgacgggtt gcgcctggat gtggtgaatc
    11881 tgatctccaa agacccgcgt ttccctgaag acctggacgg cgacgggcgt cgcttctaca
    11941 ccgacgggcc acgagcacac gagtttttgc acgagatgaa ccgcgatgtg tttacgccac
    12001 gcgggttaat gaccgtaggt gaaatgtcct ccaccagcct tgagcattgc cagcgatacg
    12061 cggcactgac aggcagtgaa ttatcgatga cctttaattt tcatcacctg aaggtcgatt
    12121 atcccggtgg tgaaaaatgg acgctggcta aacctgactt tgtggcgttg aaaacattgt
    12181 tccgccactg gcaacaagga atgcacaacg tagcatggaa tgccttgttc tggtgtaacc
    12241 acgatcagcc gcgcattgtt tctcgctttg gtgatgaagg tgaataccgc gtgcctgcgg
    12301 caaaaatgct ggcgatggtg ctgcatggca tgcagggaac gccgtatatc taccagggcg
    12361 aagagattgg catgaccaac ccacatttca cgcgcattac tgactatcgc gacgtggaga
    12421 gcctcaatat gtttgccgag ctgcgcaacg atgggcgtga tgccgacgag ttattggcaa
    12481 tcctcgccag taaatcccgt gacaacagtc gcacgcccat gcaatggagc aacggcgata
    12541 atgccgggtt tacggctggc gaaccgtgga ttggcctggg cgataactat caacaaatca
    12601 acgtagaagc cgcgctggcc gatgattcct cggtgtttta cacctaccaa aagttaatcg
    12661 cactgcgtaa gcaagaagcc atcctgacat ggggcaatta ccaggatctg ctgccaaaca
    12721 gccctgtatt gtggtgctat cgccgtgaat ggaaggggca aaccttgctg gtcattgcca
    12781 accttagccg tgagatccaa ccctggcagc cagggaaaat gcgcggcaac tggcaacttg
    12841 tgatgcataa ctacgaagaa gcctcaccac aaccctgtgc catgacttta cggccttttg
    12901 aggctgtctg gtggttacag aagtaaatct tccctaagcc ccggtaatgc cggtcattcc
    12961 ggggttttgt ctgtcagtat gttgtttttg ttgatttttc aaccagaaaa ttcattaaaa
    13021 aatttacata tcgctgtagc gcccgtcatc cgtacgctct gctttttact ttgagctaca
    13081 tcaaaaaaag ttaaaacatc cttgatgcaa agcactatat atagacttta aaatgcgtct
    13141 caacccaata tgttgtatta atcgactata attgctacta cagctcccca cgaaaaaggt
    13201 gcggcgttgt ggataagcgg atggcgattg cggaaagcac cggaaaacga aacgaaaaaa
    13261 ccggaaaacg cctttcccaa tttctgtgga taacctgttc ttaaaaatat ggagcgatca
    13321 tgacaccgca tgtgatgaaa cgagacggct gcaaagtgcc gtttaaatca gagcgcatca
    13381 aagaagcgat tctgcgtgca gctaaagcag cggaagtcga tgatgccgat tattgcgcca
    13441 ctgttgccgc ggttgtcagc gagcagatgc agggccgcaa ccaggtggat atcaacgaga
    13501 tccagaccgc agttgaaaac cagctgatgt cgggtccata caaacaactg gctcgtgctt
    13561 acatcgagta ccgtcacgat cgcgacattg aacgtgaaaa acgcggtcgc ctgaaccagg
    13621 agatccgtgg tctggtcgag cagaccaacg cctcgttact caacgaaaac gccaacaaag
    13681 acagcaaggt gattccaacc cagcgcgacc tgctggccgg gatcgtggct aaacactatg
    13741 cacgccagca cctgctgccg cgtgacgtgg tgcaggcaca tgagcgtggc gatattcact
    13801 atcacgatct cgattactcg ccgttcttcc cgatgttcaa ctgcatgttg atcgacctga
    13861 aaggcatgct gacccagggc tttaaaatgg ggaacgccga gattgaaccg ccgaagtcga
    13921 tctctacggc aaccgcggta actgcgcaga ttattgctca ggtcgccagc catatttatg
    13981 gcggcaccac cattaaccgt atcgatgaag tgctggcgcc gtttgttacc gccagctaca
    14041 acaaacatcg caagaccgca gaagagtgga acatcccgga cgccgaaggc tacgctaact
    14101 ctcgtaccat caaagagtgc tacgatgcct tccagtcgct ggagtacgaa gtaaacaccc
    14161 tgcacaccgc caacggtcag acgccgtttg taacctttgg ttttggtctg ggcaccagct
    14221 gggaatcgcg cctgattcag gaatcgatcc tgcgtaaccg tatcgcaggc ctcggtaaaa
    14281 accgtaaaac tgcggtgttc ccgaaactgg tgtttgcgat tcgcgatggc ctgaaccata
    14341 aaaaaggcga tccgaactac gacatcaaac agctggcgct ggagtgcgca agcaagcgca
    14401 tgtatccgga tatcctgaac tacgatcagg tagtgaaagt caccggttcg tttaaaaccc
    14461 cgatgggctg ccgcagcttc ctcggcgtgt gggaaaatga aaacggcgag cagatccacg
    14521 atggtcgtaa caacctcggc gtgatcagcc tgaacctgcc gcgtattgct ctggaagcaa
    14581 aaggcgatga agccaccttc tggaagctgc tggatgaacg tctggtgctg gcacgtaagg
    14641 cactgatgac ccgtatcgct cgtctcgaag gcgtgaaagc gcgcgtcgcc ccgatcctct
    14701 atatggaagg tgcttgtggc gtacgtctga atgctgacga tgatgtttct gaaatcttca
    14761 aaaacggtcg tgcgtctatt tcgctgggtt acatcggcat ccacgaaacc attaacgcgc
    14821 tgttcggcgg cgagcatgtt tacgacaacg agcagcttcg cgcgaaaggt atcgccattg
    14881 ttgaacgtct gcgtcaggca gtggatcagt ggaaagaaga aacgggttat ggtttcagtc
    14941 tctacagcac gccgagtgaa aacctgtgcg accgcttctg ccgtctcgat actgctgagt
    15001 ttggcgtggt gccgggcgtg accgataaag gttactacac caacagtttc caccttgatg
    15061 tggagaagaa ggtgaacccg tacgacaaga tcgactttga agcgccttac ccgccgctgg
    15121 cgaacggtgg tttcatttgc tacggcgagt atccaaacat tcagcacaac ctgaaagcgc
    15181 tggaagatgt ctgggattac agctatcagc atgtaccgta ttacggcacc aatacaccga
    15241 ttgatgagtg ctacgagtgt ggctttaccg gtgagttcga gtgcaccagc aaaggcttca
    15301 cttgcccgaa atgtggtaac catgacgcct cccgtgtgtc ggtaactcgc cgcgtgtgcg
    15361 gatatttagg tagcccggat gcacgtccgt ttaacgctgg taagcaggaa gaagttaagc
    15421 gccgcgttaa acatttgggg aatgggcaga taggttaatc ttcactctgc gctaagcaaa
    15481 tgccggatgc ggcgtaaacg ccttatccgg cctacatttg gcaactgctc caggcctgat
    15541 aagacgcgac agcgtcgcat caggcattga ttaccggatg cagcctacac gcggactgat
    15601 aagccaagcg aaataatgaa ttatcatcag tactatcctg tcgacatcgt caacggcccc
    15661 ggcactcgct gcaccctgtt tgtctccggg tgtgttcatg aatgccccgg ttgctataac
    15721 aaaagcacat ggcgggtaaa ttctggtcag ccatttacca aagcaatgga agacaagatc
    15781 attaaagatc tgaatgacac tcgtatcaaa cgccagggga tttccctctc cggcggcgat
    15841 ccgctgcatc cgcaaaacgt gccggatatt ctgaaactgg taaaacgcat ccgcgccgag
    15901 tgtccgggta aagacatctg ggtgtggaca ggctataaac tcgacgaact caacgctgcg
    15961 caaatgcagg ttgttgattt gattaacgtg ctggtcgacg gcaaatttgt gcaggattta
    16021 aaagaccctt ccctgatctg gcgcggcagc agcaatcagg tggtgcatca tttgcgatga
    16081 taagttccct gttacctgag tgcaggcaac agggaaatga ggaattaacg atacttctgg
    16141 tgataggcgt tgcgggtcgt tttcagttgc tctgcagcag cctgcgcttc ttttacttta
    16201 ccttcatttg ccagcttcag cgcgtcgtca atctgaccga ccagaatgtc gaaaccgtgg
    16261 cggaaatctt tcatttccgg gctgtccggt gatttatctt cgagcttcgg cggcgttgct
    16321 ttttgcgcat ccagcgctgc ggcgcgcatc ttcgttaacg cgtctttgac ttgcgccgcg
    16381 ttatccgctt tttcgatcac ttttaaattg tcgttgaggg tttccatatt gtcttcaaga
    16441 tcagcggcaa acgacgcaga actgaatacc aacgaggaga ctgcaagaat agctaacagg
    16501 cttttacgca ttgctcactt ccttttttat tatgaagatt tggcgcggca ggtcgcgcca
    16561 gtttttttac tgcccggcga ttttcatctc cggcaacagc actgaaccac actgtatatt
    16621 actgcgtgtt tcaatatcgt taccgacggt gacaatattg cgccacatat cttttaaatt
    16681 acctgcgatg gtgatttcac taacgggata ctgaatttcg ccattctcta cccagaagcc
    16741 cgcagcacca cgggaataat caccggtaat ggcactcacg ccctggccca tcagctccgt
    16801 caccaccagc ccggtgccca tctctttgag catttgttcg aagcttagcc cttgtccggc
    16861 aatccgccag ttgtgaatac cgcccgcatg tccggtgctt ttcagcccca gttttcgcgc
    16921 cgaatagctg gtcagcagcc actgagtcag gatgccatct ttaataatat cgcgacgctc
    16981 ggtgcgcaca ccttcgctgt cgaatggcgt cgacgccagc cctttcagca gatgcggatg
    17041 ctcttcaatg gtcagccagt ccggcagaat ttgtttaccc agcgaatcca gcaggaaggt
    17101 agatttacga taaaccgatc cacccgctat cgcccccacc agatgcccaa aaagcccagt
    17161 tgccacttca ttggcaaaaa tgactggcgc tttcatggtg gagagtttac gcggtgacag
    17221 gcgcgataaa gtacggcgag cacagtcggc cccaacccac tctggcgttt gcagatcgct
    17281 catcgcgcgc ccaatggtgt aggcgtaatc acgctccata tcgccatttt cttcggcaat
    17341 tacacagctg gagagcgaat gacgcgttga gcagtaaccc tgcaacatgc cgtggctgtt
    17401 gccaaaaact ttgacaccgt agtggctgtt aaagctgcca ccttcggtat tggtgatacg
    17461 tttatccgcc tgtaatgctg cctgttctgc gcgggccgcc agttcaatgg cttcatccgg
    17521 ggaaacttcc gcagggtgga acaagtcgag atctggtgcg tcaaaggcca gcagctcttt
    17581 gtctgccagg ccggcacagg gatctggcga ggtataacgg gcaatatcca gcgccgcctg
    17641 tacagtacgg gcaatggcct gcgggcttaa atcggtggat gatgcgctac ctttgcggtt
    17701 ctggtgataa acagtgatcc ccagcgcgcc atcgctattg aattcaacat tctccacttc
    17761 accataacgc gtgcttacgc taatgccggt ggtcttgctg acggcaactt ccgcaccgtc
    17821 cgatttgcct gaggccaact ccagcgcagt ggaaactgct tcttccagaa tcttgcgctg
    17881 cgcttcaact tgagagatta ctttcattgc aagtgccata atgtagagag tttctctgaa
    17941 gtctaacaga gaaccgtttt tcagtgcgca tcttaactgg taacattagc ctcttttttt
    18001 aaggagcctg agatgactaa gcagcccgaa gactggctcg acgacgttcc cggtgatgac
    18061 atcgaagacg aagacgatga aattatctgg gtcagtaaaa gtgaaattaa acgtgatgcc
    18121 gaggagctaa aacgccttgg cgcggaaatt gttgatctgg ggaaaaacgc gctggataag
    18181 atcccgttag atgcggatct gcgcgcggct attgagctcg cccagcgtat taagatggaa
    18241 ggtcgccgcc gccagttgca gcttatcggt aagatgctgc gccagcgcga cgtagagcct
    18301 attcgtcagg cgctggataa gctaaaaaac cgtcacaacc agcaggtggt gctgttccat
    18361 aaactggaaa atctgcgcga tcgtctgatc gaccagggtg atgacgccat cgctgaagta
    18421 ttgaatctgt ggcccgatgc cgatcgtcag caactgcgta ctctgatccg caacgcgaag
    18481 aaagagaaag aagggaataa gccgccgaaa tccgcacgcc agattttcca gtatctgcgc
    18541 aaactggcgg agaatgaagg gtaattttcg ttgttgatgt gagtatggtt tgccggatgc
    18601 ggtgcacctg taggcctgat aagacgcatc aagcgtcgca tcaggcacta tgctcaaatg
    18661 ccggatgcgg cgtgaacgcc ttatccggcc tacgcacact atatcaggct gaggccgaat
    18721 tactgcgcag cttccgcctt cttcgccaga ccatccagca gtttctgatg gatcccacca
    18781 aaaccgccgt tgctcatcac cagaatatgg tcgccaggct gagcggtttt caccaccata
    18841 tctgccagcg tatccacatc gccactccag tgtgcaggct gaacgcaggc ttctgccact
    18901 tctgccacct gccacggaat atgcgccggt tgcagcagga agacttcatc ggcacgacct
    18961 aatgaaggtg ccagatcgtc tttgcagatc cccattttca tggtattcga gcgcggttcc
    19021 agcacagcaa taatgcgcgc cgtaccacca actttgccac gcagcgccgc cagcgttgcc
    19081 agaatcgccg tcgggtgatg ggcaaaatcg tcatataccg tgacgccatt cgcttcacca
    19141 cgcaactcca gacggcgacg agcattaata aacgaaccca gcgcgttagc ggcatctacc
    19201 ggcgctacac caacatggcg agccgctgca atcgccatca ggccattgtg catattatgt
    19261 tcgcctacca gcgaccattt cacttcgccc actttttcgc catccagcaa aacttcccat
    19321 tcggaagcat cggtggtcag ctttttcgcc tgccagtgac cctgctcacc caccagctcc
    19381 tgctcgctcc agcagcccat cgccatggtc tgtttcaggt tgatgtcatt ttccggccag
    19441 ataatacggc cctgccccgg aacgatacgc accagatggt ggaactgttt ctggatcgct
    19501 ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt tgttgaggat cagcgtacgc
    19561 gggcagtaat ggacaaattt agagcgtttg tcgaagaagg cgcagtcgta ctcatccgct
    19621 tcgataacaa agaagtcgct ttcgcccaga cgcgccgaaa cctcaaagtt ccccggcaca
    19681 ccgccgatca caaatcccgg tttgtaacca cactgttcca gaatccaggt cgccattccc
    19741 gcggtggtgg ttttgccgtg tgtaccggca acggccagca cccagcggtc gcgcagcaca
    19801 aaatcgtgca gccactgtgg acctgacata taagggatgt ttttttccag taccgcttcc
    19861 acacacggat ttccacgggt catggcgttg ccaataatca ccagatccgg ctgcggctcg
    19921 agctggctgg catcgtaacc ctgaatcagc tcaatgcctt gcttctcaag taaggtgctc
    19981 atcggcggat acacattggc gtccgaaccc gttacttcat ggcctaactg gcgcgccagc
    20041 atcgccagac cgcccataaa tgtgccacaa attcctaaaa tatgaatgcg catacgtcac
    20101 tatccttttt taatctgccg ctcattttac gcatatgtcg ggcaagtgag aaatgcattt
    20161 caggataatc cgcaatttgc tggcgcgatt cacctgagcg caacattgag attatttgtt
    20221 aagattgttg cggtcgcttt actccataaa cattgcaggg aaagttttat gaaaacgtta
    20281 ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg ctaccggtga gctcactgct
    20341 ttgctgtcgg caataaaact gggcgccaag attatccatc gcgatatcaa caaagcagga
    20401 ctggttgata tcctgggtgc cagcggtgct gagaacgtgc agggcgaggt tcagcagaaa
    20461 ctcgacttgt tcgctaatga aaaactgaaa gccgcactga aagcacgcga tatcgttgcg
    20521 gggattgcct ctgaagaaga agatgagatt gtcgtctttg aaggctgtga acacgcaaaa
    20581 tacgtggtgc tgatggaccc cctggatggc tcgtccaaca tcgatgttaa cgtctctgtc
    20641 ggtaccattt tctccatcta ccgccgcgtt acgcctgttg gcacgccggt aacggaagaa
    20701 gatttcctcc agcctggtaa caaacaggtt gcggcaggtt acgtggtata cggctcctct
    20761 accatgctgg tttacaccac cggatgcggt gttcacgcct ttacttacga tccttcgctg
    20821 ggcgttttct gcctgtgcca ggaacggatg cgcttcccgg agaaaggcaa aacctactcc
    20881 atcaacgaag gaaactacat taagtttccg aacggggtga agaagtacat taaattctgc
    20941 caggaagaag ataaatccac caaccgcccg tatacctcac gttatatcgg ttcactggtc
    21001 gcggatttcc accgtaacct gctgaaaggc ggtatttatc tctacccaag caccgccagc
    21061 cacccggacg gcaaactgcg tttgctttat gagtgcaacc cgatggcatt cctggcggaa
    21121 caagcgggcg gtaaagcgag cgatggcaaa gagcgtattc tggatatcat cccggaaact
    21181 ctgcaccagc gccgttcgtt ctttgtcggc aacgaccata tggttgaaga tgtcgaacgc
    21241 tttatccgtg agttcccgga cgcgtaatct cctgcgcctc tccacacgga gaggcgtttt
    21301 tatcgctgcg caatgttgac tcttgtcact ggcgaactct gacgattctc ccacagcacc
    21361 gttaatccac gctgtaatgc tataaaaata aacaacaaaa taccgatggc gattttcgtc
    21421 caccatgagc tcagcgtgcc atcaaagttt atgtaagtct gaatcagtcc ctgaatcgcc
    21481 acgccaaaaa gcgtccctaa taccgttcca acgccaccgc tcagaagcgt accgccaatt
    21541 accactgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata tccggcctgg
    21601 gtataaatcg agaagacaat ccccgccagc gttgccagtc cggtggagag catataaatg
    21661 cgaatagtgg tgctgcgagt ggaaatcccc atcaggttcg ccgacgttgc gttgccgcca
    21721 atggcgtata cctgattacc aaaacgggta cgatgcgcga ggaaaatacc gataaccacc
    21781 accgccaaca tcagcagtcc catcgcactt aagcgaccgc cgccagggat tttccacgca
    21841 aggcttgaga gcgtgtcata aatgggatgg tttatcggaa tcgactcttc cgaaacgaga
    21901 tagctgacac cgcgcaaaaa gaacatcccc gccagggtaa tgataaatgc cgggatcttc
    21961 agggcgtcga tcaaaagccc cataaatgcg ccgaaggcac agcccatcac cagcactagc
    22021 ggaaacgcca gtagcggcga gaggccgaaa tcgccaatca cttttgccag aaacacgcca
    22081 gtaaaggcga tcaccgaacc gacggagaga tcgatcccac cggagaggat cacaaaggtc
    22141 atgccaacgg caatgatccc aagaaaggcg ttatcggtca ggatattgca gatcactctt
    22201 gtggaagcaa aaccgggaaa ctgggtcagg cagtaaagat aacccaacac aaagacgccg
    22261 atggtgatca tcagtggcaa attacgtttt atcacggcta cgtactcctt taatcagact
    22321 gataaagcgt tgcgactgga caatcagcac gcaaagaacc accaccgctt ttacaacctg
    22381 gttcatctct ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct gaataatcag
    22441 cgcccccacc accgaaagca gtaggttaaa acgtccgccc atcagcgatc cgccgccaat
    22501 aaccaccgcg agaatggcgt ccagctccag ccataacccg gcgttattgg catcggcacc
    22561 gcgaatatcc gccgccacga taatgcccgc aatcgccgca cacagcccgc tcaacacgta
    22621 ggtaagcatg acgatgatcc gcgtgtttac cccggcattt tttgccgccc gaatgttgat
    22681 accaacggct tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc agaacaggat
    22741 aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc cactgccaaa
    22801 ccatgagaga tccggcgagt taaacgtgac gatctgtccg gaggtgatca gttgcgccac
    22861 gccgcgcccg gcgaccatca ggatcagggt ggcaacaaac ggctgaattt tgaggatcgc
    22921 taccagtatg ccgttccaca atcccgccag gatgccagtg cccagggcgc ttaacaaaac
    22981 aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg cgatagccat
    23041 caccgccccc acggagagat caatcccacc tgttgcgatc accagcgtca ttccgatagc
    23101 caacagcgca acgggggccg cacggttaag aatgtctatg gggctaccga ataaacgccc
    23161 atcctggagc accacctgcc agaaatgcgg ggccaccagg ctatcgacca gcagcaccag
    23221 caacagttcc gccagctgcg gcattcccgt tggccagcga aagcgccttt tcggcggcgt
    23281 agtgtccggg agagattgag gcatcacact gttctcctta cgccgcaatg gcgttcatga
    23341 tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga tcgcgcatga
    23401 tgatcacccg gtcggcatag cccaccagtt cttccagttc ggaggagatc accagcagcg
    23461 ccagaccatc ggcgcatagc gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat
    23521 caatgccgcg agttggctca tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg
    23581 aaagcaacac tttttgctga ttgccgccag agagaaattc aatcggttgt tcagttgaag
    23641 gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgt tctttgcggg
    23701 aaatgggacg tagccagccg cgctgggcct ggagagcgag gatgatattt tcccgcaccg
    23761 aggcggcagc gatgatgcca tcggttttcc tgtcttccgg gcagaagcca atgcccagta
    23821 ccgaagcctg atgtggcgat cgcaggtttt gcggtttgcc tttgatcaac gccgtgccgc
    23881 tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtcct gatcccagca
    23941 atccagccag accgacgatc tcgccggggc gtacttcgag atcaaacggt gcgatcgttc
    24001 cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg
    24061 cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc agttcgatct
    24121 gcggtagctc gcacgtttcc cgacagccta cgaaactgcc gttgcgtaag acggtgatcc
    24181 gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaaaatcagg ctgacgccgc
    24241 gatcgcgcaa ctgacgcatc aggtcaaaca gtaactccac ttcctgggtg tcgagactgg
    24301 cggtgggttc atcgaggatc agcactttgg cagagagatc gatagcccgg caaatagcga
    24361 cgatttgctg catcgcgact gaaaagcggt tgagcggttc gcgcacgtcg agggagaaac
    24421 cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttgcggcgt agaaggccga
    24481 agcgtttggg ttcgcggcct ataaatagat tatcagcgac cgacatattg ggtagcaggt
    24541 ttacttcctg atagacggtg ccgataccga gttgttgtgc atgggcggta tttttcggtg
    24601 agatagcctg gccttccagc cagatggtgc cgcgatcggc gtggtataca ccagttaatg
    24661 ctttgattag cgttgatttt cctgccccgt tttcaccgag cagcgccatg atttcgccgc
    24721 ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc
    24781 cttcggtgcg gaggatctcc tggtgttggt tagtcgtcat ggtttccccc ggtgtaaagc
    24841 gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca gcgcaccacc
    24901 gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct
    24961 caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg cagataaagg
    25021 gtggatttgg tcagcgtcag cttttcaggc atggtgccgt cttttttgta tttctccagc
    25081 gcgtcgaagg cgggacctgc catattcggc gtcagttcaa cactggcgtt cgcttcgcca
    25141 tcaatcatcg ctttgtagat atccggtacg ccgtcgatag aacctgtcag aatatctttg
    25201 cctggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat gtcgtcgtta
    25261 tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctctccatg
    25321 acttctttgc ctttactgcg ggtgaagtca cctgactgcg agcggataat tttgatattt
    25381 ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac gctggcccca
    25441 acagtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt taccagccag
    25501 tcaccaatca acttgccttc gagaatgttg tcggcagtga cggtggtcat atagagagat
    25561 ttgtctttca catcaataga acgatcgagc aagaagaccg ggatttcggc atctttcgcc
    25621 tcttttaata ccggttccca accagtcgca accaccggag cgataaagat cgcatccacc
    25681 ccttgcgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg ctgaccatcg
    25741 gcaattttca gcgtaattcc gcgtttttcg gcctcacttt tcgccacatt ggtttctgcg
    25801 gcgcgccagc cagattccga tccgacctgc gaaaatccta cggttaatgg agcggccaac
    25861 gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg
    25921 ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa
    25981 ttcgttatgt aacggattgc gttacatcac acttcaaaga agtaaataaa acgttaatca
    26041 caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct
    26101 gctctgtata ccgtcttaaa attggcgaaa aaggaaaatg aagacgaaaa caagcgaaga
    26161 cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt ttaaaggaaa
    26221 cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg
    26281 ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa gagagcggcg
    26341 cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggttaca
    26401 tcaaccacac cctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc
    26461 cgctgcagcc gggttctgtg atccgttgcc gtccggttgg cgttctgaaa atgaccgacg
    26521 aagccggtga agatgcgaaa ctgattgctg ttccgcacac caagctgagc aaagaatacg
    26581 atcacattaa agacgttaac gatctgccag agctgctgaa agcgcagatc gctcacttct
    26641 tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg
    26701 cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgcaaagaat aaataagttc
    26761 ttctagcgca ataaccctga acgccgggct tcggttagta agggtttttt tatgcccgcg
    26821 ataaataaac tctctattcc accatcatta ttctcagcgg ttgcaaggct tgaacggtaa
    26881 gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa gttagccgac
    26941 ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc
    27001 cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga
    27061 tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaaggt
    27121 ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa
    27181 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa
    27241 ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
    27301 gtcaatacga taaacttcac cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg
    27361 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
    27421 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
    27481 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
    27541 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
    27601 acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag
    27661 tcgcctactc cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
    27721 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
    27781 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
    27841 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
    27901 cgaactccgg cgattacgtc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
    27961 agatacggtt gatccggcgg accagagaac agtaactcac ctttacgcac aatcagatcc
    28021 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
    28081 tgtttgtcct gaacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
    28141 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
    28201 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
    28261 gcgctttccg gcagatcgtg caccacgatg cgcgcccaca gaacatccac gcgaccatcg
    28321 agggtaaaca ggtttggtgt agcctcgaag acaacatctg gcgatacatc cattcgtacc
    28381 atcggcggca cggtgatccg cactttactg cctttcgccg ttacccgcgc ccgccagttt
    28441 tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact
    28501 gtaccggcaa gcgtcgagcg cataccatta aagttgaccg caagctggct cggttgcata
    28561 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
    28621 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
    28681 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
    28741 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
    28801 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
    28861 agattcagtg tctgaaacgc cacgggcagc gccgcatcgt tgacggtttg cgttacctgc
    28921 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttggt ggtgtcccag
    28981 gcaacgtccg ctttacccgt gaagataccg ctggcctgag tggtttctgg cataaatggt
    29041 ttcagcatgg ccaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcacccgca
    29101 tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg tggcccgatg
    29161 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag caaccacggg
    29221 ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc ttctttgcga
    29281 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
    29341 aactcgtgct gtttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
    29401 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
    29461 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
    29521 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
    29581 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
    29641 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg ccccagttcc
    29701 agatgaagcc ctgggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
    29761 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
    29821 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactct
    29881 gggatctctt tggcggtgtt aatgccgtta agcgttagct caccgcgcca actaatggcc
    29941 tgctgccagt cgagcaacgc cttgagttca gttttccctt ccagcgccgc gacggtgagt
    30001 ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt ggctggcggg
    30061 atctccagtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
    30121 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaatc
    30181 tgtttgctgt tcacttccac gttgagcggc aatccggctt ccgccagtcg cgtctgggcg
    30241 cgtaaatcca tatccaccgg accggaaaga ttaacgccaa tctccagctg ttcgcgcagc
    30301 gcgccgccca ctttcagctt cactttttca cctttcaacg gctccacgtt cagtgtgctg
    30361 ttgagggtga tatccaccgg ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg
    30421 ctcccctggt tggaatcgat atccagggcg tccagtttag tattgccgtc aatgctgctc
    30481 actttcagca gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg caactgctcg
    30541 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
    30601 accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc cggctgcgga
    30661 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccgccacttt cggcagagca
    30721 atcagcaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcaga
    30781 ccggaggtga agtccatcac cgataccgtg gtgtcatcaa tcttgatgtt gacgttatcc
    30841 agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accactatct
    30901 tcctcttctt caacctgttc agaagggggc atttttttac tgtcgatgtt gacctgaatg
    30961 tctttcagcg ccaggtcatt aatacaaacg ctactgttcc aaagacaatc cagcccgacc
    31021 gccagatgca gattgcccgc ttttaccgcc acgcctggct gctcataacg aacgtcagac
    31081 aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg cacccagcga
    31141 tccgccgctt taaataccag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
    31201 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
    31261 attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc gcgaccggta
    31321 cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
    31381 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
    31441 cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt atcaacttcg
    31501 aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat ttgtagccgc
    31561 gaatactgcg atcgcccccg gcgaagaaac gcagatccgg cggtactttg tcgaaatcac
    31621 cggtttcaat ccagcccagc gtgccgcgtg taacaaaacg atggcgatcg tacagtgtgc
    31681 ggatccagac gttttgtgcc tggaaaacgg agaaatcgac atctgagccc caggccgtgt
    31741 tggagtagtc gatagagtag cgttgcgagt cgccccaggt tggcatcaag ccaccacgag
    31801 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
    31861 cctgagtaaa gtggtcgaga ctccagcgca ggttaatggc acgctgccag ccgctggaga
    31921 gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag
    31981 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagtggca
    32041 ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg gtggtcagac
    32101 tgtgaccata agagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt
    32161 ccgtagagta accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg
    32221 tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
    32281 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcgtact
    32341 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    32401 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    32461 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    32521 ctttacgcaa cgcaatgctg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    32581 gtaccgtacc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    32641 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacgcct ggcgtgactt
    32701 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    32761 gctggtaata acccagcgct ttcagacctt cgcggatggc atcatcgacg cgtgcgcgaa
    32821 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgcg cgaacgttct
    32881 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    32941 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    33001 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    33061 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    33121 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc
    33181 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    33241 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    33301 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    33361 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggag
    33421 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    33481 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    33541 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    33601 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    33661 gttttcaggc ggcgatgctt gccgccgatg atgatcgtca catcaccacg gaaatcgcta
    33721 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccgt
    33781 atggttactg tggaattggc ggaattggcg tctgtctgcc accggaagca tagcgttacg
    33841 ggtacaaatg tagattgttg ataaagtgcg ctttatttat gccagatgcg gcgtaaacgc
    33901 cttaccaggc ctacaaaatc gtccaaattc aatatattgc aaggactgcg taggcctgat
    33961 tggcatagcg catcaggcag tattgcattt atcagctgtc tgaattttaa ccctctggcg
    34021 actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    34081 ggctttcaat gtgtatttca cacaaattaa tcaacttccc cttccgagga tctggcctga
    34141 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt
    34201 gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg
    34261 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    34321 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc
    34381 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagaa
    34441 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    34501 tttgcggatt taactccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt
    34561 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    34621 ggcctggcga acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    34681 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa
    34741 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca
    34801 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagtctgaag
    34861 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    34921 atcatcggtt atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    34981 gcactgaaca gcggcgtaca aattcgcaac acgctgattg taccggatac gttaaccctt
    35041 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    35101 tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat
    35161 ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    35221 gacggcggca ccccgattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag
    35281 tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa
    35341 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    35401 cgtatcgacc aactgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa
    35461 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct
    35521 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    35581 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    35641 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg atgtataacc
    35701 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    35761 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    35821 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    35881 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    35941 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    36001 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    36061 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    36121 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaaccggtc agcgagtgcc
    36181 gccgattggc attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    36241 aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    36301 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    36361 gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcaattc cgggttccgg
    36421 catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    36481 tgttgatagc aatggcgtcg cacgcggtgc ctggcagctg gatgaaaaaa gttccgctgt
    36541 agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc ttactcagga
    36601 agaggtgcag caagtgatgg acctgctgca taaattaatt aataaataga cactctgaac
    36661 cccggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg ccagtcgtga
    36721 acgtgcgctc cggcagctgc agcgacagca tgtccagcgg cggtgtccca aatattcgtt
    36781 ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca ccaggcagaa tttcagcgaa
    36841 gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt cagctccgca
    36901 tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac ctgaatctgc
    36961 ttgcgcacac cgcactcttc tttccacgct ttgccttctg ccgcgctgta cattacgttc
    37021 attaccggcg catacaccac gcctaaaatc ggtttgccat ggtcaatgag cgcaatgtta
    37081 acggtgaatt cgccattacg tttaataaac tctttagtac catccagtgg gtctaccagc
    37141 cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc agaaaggacc
    37201 ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgtgc ggcaatatcc
    37261 gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg
    37321 tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg atctaacatt
    37381 tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc ctatcgttaa
    37441 tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg gcattcttca
    37501 ctgttctata agtaagacgt ttattctccc ttttctttcg tattcccgat gataaaagga
    37561 tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc gccagtgtga
    37621 atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc aacatgatgg
    37681 atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt acggcaagcc
    37741 tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac ggcgatttga
    37801 ttcaggggag tccactggcc gattacatgt cggcgaaatg attaaaagca ggtgatgttc
    37861 atccggttta taaggcgctg aatacgctgg attacacggt cggtacactc ggcaatcatg
    37921 aatttaacta cggtctggat tacctgaaaa atgcgctggc gggagcgaaa ttcccttatg
    37981 taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg tatttaatta
    38041 aagataccga agtggtcgat aaagacggaa aaaaacagac gctgaagatt ggctatattg
    38101 gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa tttatccgga aaagtgacgg
    38161 tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag aaaggtgccg
    38221 atgtcgttgt cgttctggcg cattccgggc tatctgccga tccgtataaa gtgatggcgg
    38281 aaaactcagt ttattacctc agtgaaattc ctggcgttaa cgccattatg tttggccatg
    38341 ctcacgccgt tttcccaggt aaagattttg ctgatatcga aggggctgat atcgcgaaag
    38401 gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg gggcgatcat cttggggtgg
    38461 tcgacttaca actcagtaat aacagcggta aatggcaggt gacgcaggcg aaagcggaag
    38521 cacggccgat ttacgacatc gccaataaaa aatccctcgc ggcggaagac agcaagctgg
    38581 tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag ccaatcggta
    38641 aatccgccga caatatgtat agctatctgg cgctggtgca ggacgatccg accgtgcagg
    38701 tggtgaacaa cgcgcaaaaa gcgtatgtcg agcattacat tcagggcgat ccggatctgg
    38761 caaaactgcc ggtgctttca gctgctgcac cgtttaaagt tggtggtcgc aaaaatgatc
    38821 cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt
    38881 atctctaccc caatacgctg attgtggtga aagccagcgg taaagaggtg aaagagtggc
    38941 tggagtgctc cgccggacag tttaaccaga ttgatcctga caacacgaaa ccacagtcac
    39001 tcatcaactg ggatggtttc cgcacctata actttgatgt gattgatggt gtgaattatc
    39061 agattgatgt tacccagcct gcccgttatg acggcgagtg ccagatggtt aatgccaatg
    39121 cggaaaggat taagaacctg acctttaatg gcaagccgat tgatccgaac gccatgttcc
    39181 tggttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg ggcgacagcc
    39241 atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcgtgg attgctgatg
    39301 agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt ttagcaccga
    39361 tagctggcga taagaaactg gatatccgtt tcgaaacctc tccatcagat aaagccgcgg
    39421 cgtttattaa agagaaaggg caatatccga tgaataaagt cgcgaccgat gatatcgggt
    39481 ttgcgattta tcaggtggat ttgagtaagt aatacacttc tttttcggcc tataaatcat
    39541 caaccgcatc cggcactttt gtcggatgcg atgctggcgc atcttatccg ccctacaagt
    39601 catgaaccgt aggccggata agcgcagccg gcagttatgc cgcacgttca tcccgcaccg
    39661 ccaacacctc gggcaaattc aactcaatcc agtccgccag tgcagcaacc ttttcgctca
    39721 cctgctcgcc cagcggcgtg aggctatatt ccgcatgcgg cggcaccacc ggatacgcga
    39781 tacggttaag aaaaccatcc tgttctaacg cctgtaacga ctgcgcaagc atcttttcgc
    39841 tcaccccgcc gattttgcgc cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca
    39901 ccagaatcaa caccccccaa cggctggtga cgtgtttcaa cacctcgcgc gacgggcact
    39961 gttccgcaaa gagattaccc tctttcagtt gttgcgacag gctaacctga ctcattccat
    40021 acttaccttt ttgtgtgtac ttactaaaag taagtttagg tgttagcgta tttaaacaca
    40081 agacaaaacg atggagactt cccatgatcg ctattactgg tgccactggc caacttggtc
    40141 actatgttat tgaatccttg atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc
    40201 gtaatccggc aaaagcccag gccctggcag cacaaggcat taccgtgcgt caggctgact
    40261 acggcgatga agccgcactg acatctgcac ttcagggagt ggaaaaacta ctgctgatct
    40321 cttccagcga agtgggtcaa cgtgccccgc agcatcgtaa tgttattaat gccgcaaagg
    40381 cggctggcgt gaaatttatc gcttatacca gcctgctaca tgcagatacc tccccgctcg
    40441 gcctcgccga tgagcacatc gagacggaga aaatgctggc tgattctggc atcgtttaca
    40501 ccctgctgcg caacggctgg tacagcgaaa actacctcgc cagcgccccg gcagcactag
    40561 aacacggcgt atttatcggt gcggcgggcg atggcaaaat cgcctcagca acgcgggcag
    40621 attatgcggc agctgcggca cgcgtgatta gcgaagccgg tcacgaaggc aaggtttacg
    40681 aactggcggg cgatagtgcc tggacgttga cacagttagc ggcagagctg accaaacaga
    40741 gcggcaaaca ggttacctat caaaatctga gcgaagccga tttcgccgcg gcactgaaaa
    40801 gcgtcggact gcccgacgga ctggcggata tgctggcgga ttctgacgtt ggcgcatcga
    40861 aaggcggtct gtttgatgac agcaaaacac ttagcaaatt gattggccac ccaacgacaa
    40921 cgttagccga aagcgtaagc catcttttta atgttaataa ctagttaatt aaagtggcat
    40981 cctcccgcat cctctctgat aatgacggga tgccgggagc aatcatgtct gcttcctgaa
    41041 ctttcttctg acagatcaat ggatgccagt aatgattagc ggcgtgctgt acgccctgtt
    41101 agcagggttg atgtgggggc ttatttttgt cgggccgttg atcgtgccgg aatacccggc
    41161 gatgttgcag tcgatggggc gttatctggc gttagggtta attgcgctgc ccattgcctg
    41221 gctgggacgc gtgcgtctgc gtcagttggc gcgtcgcgac tggcttaccg ccttgatgct
    41281 cactatgatg ggcaacctca tctattactt ctgccttgcc agtgccattc aacgtactgg
    41341 cgcgcctgtt tccacgatga ttatcggcac cctgccggtg gtcattcccg tcttcgccaa
    41401 tctgctttat agccagcgcg acggcaaact cgcgtgggga aaactcgccc cggcactgat
    41461 ttgtattggc atcggcctgg cgtgtgtgaa tattgctgag ttaaaccacg gactccccaa
    41521 ttttgactgg gcacgttata cctctggcat cgtgctggcg ttagtttccg tggtctgctg
    41581 ggcgtggtat gccctgcgca acgcccgctg gctgcgggaa aatcccgaca aacatccaat
    41641 gatgtgggcg acggcgcagg cactggtcac gctgccggtt tctctcatcg gctatctcgt
    41701 cgcttgttac tggctgaata cgcaaacgcc ggacttctcc ctaccctttg gcccccgtcc
    41761 gctggtgttt attagtctga tgattgcgat agccgtgctt tgctcatggg ttggcgcact
    41821 ctgctggaac gtcgccagcc agcgattacc gacagtgatt ctcgggccgc tgattgtttt
    41881 cgaaacgctg gcaggtttgc tgtacacctt tttactccgc cagcaaatgc cgccgctaat
    41941 gacgctgagc ggtatcgcgc tgttagtgat tggcgtggtc atcgcggtca gagcaaaacc
    42001 agaaaaacct ttaactgaat ctgtctcaga aagttgacac gctggcagtg agttaaataa
    42061 gtctctgcta cgtaagggtt atagcttttg ccttaaagat gcatttaaaa tacatcttat
    42121 cttattaaga atgaggtatc agctatggct tatcgcgacc aacctttagg tgaactggcg
    42181 ctctctattc ctcgcgcttc agctctgttt cgtaaatatg atatggatta ctgctgcggc
    42241 ggtaagcaaa cgctggcgcg tgcggcggca cgtaaagaac tggatgttga ggtcattgaa
    42301 gctgaactgg caaagctcgc tgaacaaccg attgagaaag actggcgtag cgccccgctg
    42361 gcagaaatca tcgaccatat catcgtgcgc taccacgatc gtcaccgcga gcaactgccg
    42421 gagctgattc tgcaagcgac taaagtcgag cgcgttcacg ccgacaaacc gagcgtgcca
    42481 aaagggctga caaaatacct gactatgctg catgaagagc tttccagcca catgatgaaa
    42541 gaagagcaga tcctcttccc gatgatcaaa caaggcatgg gcagccaggc aatggggcca
    42601 atcagcgtaa tggaaagcga gcacgatgaa gcgggcgaac tgctggaagt gatcaaacac
    42661 accaccaata acgtcacacc gccgccggaa gcctgcacca cctggaaagc gatgtataac
    42721 ggcattaatg aactgattga tgacctgatg gatcacatca gcctggaaaa caatgtactg
    42781 ttcccacgcg cgctggcggg tgagtgatct aaaagctgga tggcattgcg ccatccagca
    42841 tgataatgcg gttatttccg cagttcagca gcccgcttct taccaataaa caaccagccc
    42901 agccccagcg cgataaacca cagcggagtg accagcagcg cctggcgagt gtcatcttcc
    42961 agtgtcagca acacgaccac gaacacaaag aacgccatac atacccagca catcagcttg
    43021 ccgagcggca tcttgtagat cgacttctca tgcagatgag gacgctgttt gcgatacaca
    43081 aggtacgagc aaaggataat tgtccagacg aacataaaca gaatcgcgga aacggttgta
    43141 atcatcgtga acgcgccaat cacgctagga ttcacataca acatcaccac accgccgagc
    43201 aggcagatgc aggagaaggt cagccctttc gctggtactg cgcgcttaga aagtttagcg
    43261 aacgctttcg gtgccacacc ttcctgcgcc agaccaaaca gcatacggct ggtagagaag
    43321 acgccgctgt tagcggaaga cgccgcagag gtcagtacca caaagttgat cacacttgcg
    43381 gcagcaggca gccccaccaa cacgaacagt tcaacaaacg ggcttttctc cgggactacc
    43441 gaactccacg gcgtcacgga cataatcaca atcagcgcga agacgtagaa cataatgata
    43501 cggatcggaa tggagttaat cgcgcgtggc agtgatttct ccggatcttt ggtttccgca
    43561 gctgttgtac ctaccagctc aatccccacg aaagcgaaaa ccgctatctg gaatccggca
    43621 aagaagccac ttaaaccttt cgggaaccag ccgccatcgt tccacaaatg cgcgaatgac
    43681 gcttccacac cagtcggtga ctgaaagtgc atcgccacca tgaccaggcc gacgacaatc
    43741 agcgacacga tggcgacgat tttgatcatc gcaaaccaga actccatctc accgaacatt
    43801 ttcacggtgg caaggttgag cgtcagcagc agcactatca ccgccagcga ggcgacccag
    43861 tcggagagat cggggaacca gaactgagca taagccgtga tcgccaccac gtctgccata
    43921 ccggttacaa cccagcagaa ccagtaagtc cagccggtga aatatcctgc ccacggcccg
    43981 agtaaatcgg aagcgaagtc actaaaagat ttgtattcca gattcgaaag cagcaattcc
    44041 cccattgccc gcatcacgaa aaagagcata aaaccaatga tcatataaac gaaaatgatc
    44101 gacggcccgg caaggctaat cgttttgccg gaccccataa acaaccccgt accaatggca
    44161 ccgccaatgg caataagctg aatatgtcgg tttgtgagat tgcgccgtag cgactgttca
    44221 gccggagcct gatcatcggc aacgactttt acctgatcta ccatgttttt ttcttcctgt
    44281 acctgtctgt gttgttcagg ctctacggcc tttagtcagt ctatgatacg acgaatctgt
    44341 atcgggactc atcgatatta ggtaacaata cgcgggatga atactaagat ttagatctaa
    44401 tgttaatttt atgtttaaag tgagtgtcat atcacggtga taacgcgaaa cagctcacaa
    44461 aaatacacgc ttacggggtc tttgcaacac aaaatagcgc cgtttggcaa actgaagggt
    44521 ttattgctgc atgcctgctc ccctctggtt cgaggggaac agaatgcgct gcttagagga
    44581 tttccagcag ttcgacttca aacaccaggg tgctgaacgg agggatggac gcgcctgcgc
    44641 cacgctcgcc atatgccagt tcctgcggga tagtcagttc ccatttagaa cctaccggca
    44701 tcagagtcag tgcttcaatc cagccaggga tcacgccgtt aaccgggaat tcagcgggtt
    44761 caccacgagc aacggagctg tcaaacacgg tgccgtcgat cagtttaccg gtgtaatgaa
    44821 cacgaacgcg gtcggtacgt gccggaattg cgccttcacc ctggttgatc acgcggaatt
    44881 gcaggccaga ttcggtgctg ttcacacctt cttttttggc attttcttca agatatttca
    44941 caccttcagc agccatcgcc tggaaacgct gacgacgaac ggcatcggcg cgttcgtgga
    45001 tttcacgcag tgcgcgatgc accacatcaa ccggaacagc cggatgtttg ccttccagcg
    45061 catcggcaat acctgcaacc agtgcttctg gcagcagccc ttccaggcca gattcactca
    45121 gttgttgccc tacctgcaaa ccaatgccgt agcttgcttg cgcttcgatg gtgtcaaaag
    45181 ttggggtggt catcgtattt cctttcttag ggtataaaag tagcgggcag catatcagcc
    45241 acacttcttt gggtaaaact ttgtcgcagg aatgatgaca aatcccgggg aaaaagaaac
    45301 aatagcaata tcccgtatga atataataaa ggcgggttta ccacggacat catagcgtta
    45361 gcttattcat aggctatgat tgaggaacaa gacgcggagc aggaggaaac catgcccggg
    45421 cgctttgaac taaaaccaac cctggagaaa gtctggcacg cgccggacaa ttttcgcttc
    45481 atggacccgc tgccgccgat gcatcgtcgg gggatcatta ttgccgccat tgtattggtg
    45541 gtcggatttc tgcttccatc tgatgatacg cccaacgcgc cggtggtgac gcgtgaagcg
    45601 cagttagata ttcagtcgca atcccaaccg ccgacagaag aacagctgcg ggcgcaatta
    45661 gtcacaccac aaaatgatcc tgatcaggtg gctccggtcg ctccagaacc tatacaggaa
    45721 ggccagccag aagagcagcc gcaaacttcc caaacgcaac cattccagcc agatagcgga
    45781 attgataatc aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc
    45841 cgcgaccacg gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt
    45901 aagccgctga gtaatttgca aaacgggcag atggtgaaaa tccgccagaa cgccagcggc
    45961 gtggtgacgg gcttaaccat tgatacgggg aataatcaac aggtcctgtt cacccgccag
    46021 ccggatggca gttttattcg tgcgcggtag atgacgggtc aaccaaaagc cagctaagga
    46081 ttgtctcttt gatgacctta cgtctggctt cctgtgcctg gggtgaaacc atactgatgt
    46141 gttgacatcc tccacgccct gaaggacgtg gatccctgct acgttcaggc tgtcgcctga
    46201 atcatttcgg tgggttcctg cttcaacggg cggcctgact gcaccatctc tccacaggca
    46261 agaacggcgt gccccgccgc taaaatgtta cgagcgccat ttacatcagc gttcgctgta
    46321 tatccacatg cctggcatct gaatttactt tgtgacaggc gattttcttt cgctgtatga
    46381 ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca
    46441 cgccagagct gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc
    46501 gaacggttta aacctgattt tgcccggaca ttgcgccccg gctggctgac cgtacccgct
    46561 gctgactttg acatgttgct gaccttcaaa tcctcaatga ctatcattgc gtggtttttg
    46621 ctgacggtcg ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acgggaatgc
    46681 agtcgctgta ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttgcggctt
    46741 aactgtcgct gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt tacaggccca
    46801 aagactgtgc catctgacag cgtggcgagt ttagccacgc cagcatccag tccgaccatt
    46861 aatgctgaag ggtgaaccgg agttgatact tcattttcag tctgaatact gatgtaccac
    46921 ttaccgcagg actggctgac agtgacattt ttcacaactc ccgtaacctg acggctattc
    46981 cggtagcgca tccagccaag tttcggcaga aaaatacggc tgttttcctg atcgagctta
    47041 acgccctgcg ggtagcggaa tgcatcattc tgtccccgct ttttgaatcg gggaaaagca
    47101 gcccgattct ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga ctgttgcaat
    47161 ggctgtgagg gagaatcttt aagccattgc gtttcagtgg cgtttttcca ctcaaccagc
    47221 caggaagcca ttttcccgta agggatgtat ttattaccgg cctcatgatt ctcattctga
    47281 agcgccagag cacgattgaa aacgaaacga catgcgcctc atttcacgct cctgttgacc
    47341 acctggtctt aactggaatt taaatgcctg tagtcgtttc acaggccaaa tgatactttg
    47401 gtctgtgaaa aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt
    47461 tcatctggtc tttgtcacca gataccgacg tcagattttt gactatgacg cgacagaaaa
    47521 actacgcact tacttttcaa atgtatgtgc tgattttgaa actgaactgg ttgaaatgga
    47581 tggcgaacca gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag
    47641 tctggtaaac agcctcaaag gcgtatcgag taggttactg cgacgagatc gaccagatat
    47701 tgcagtcagg tattactaca aaggcgtttt gtggagtcct ggctattttg ccagtagctg
    47761 cggaggtgcg ccaatatccg ccatccgtca atacattgaa caacagcaaa cacctggtca
    47821 ggtggaaaac cgcgccgtat atccccgccc tgaaggacgg ggttttacgg cgcaccggat
    47881 aaaccaggcc aagcgtatgc tggttgatca aataatatcc gccaagcgca gcaatattaa
    47941 tattcacctg caatgggtcg atatctggct taaaaatatt gtgtttcttc ccttcatcca
    48001 ataaggattc cattaactgc aagtgcgcat ggttaatttc taagagacgc tgtgattttg
    48061 cataatgcac acctttactt tgattttcac tatgcacaat ttttaaaaac caaggattag
    48121 caatataata atcccatgta aaatcaataa ttttctcaat ggctttttgt ggctggctaa
    48181 cctgctgatt aacgaataca ccgataccga cagcgtagaa taattacata ccgcacaaca
    48241 agaatggtaa ggcgtggaag tcattttctc cccgggtgca tcgttatatc cataatgagc
    48301 gatttttgat attaatatct ttcaccatcc gctattttca ccacttcaga aaacaaactt
    48361 atttttatta atatcaattc tttttctttg aacattcagc tgcgatattt gtaaaaatat
    48421 taaacaggta acattacaat gacattacca accactattt attcatttcc tgcatattta
    48481 tctcgttttt cgtccaccga caaaccagta aaattaaaat ttcatcaata cgcacgcgct
    48541 acattacttt caaatcgtgg aagagatcat aattgcgatg gcagacgtac tgttgagatc
    48601 cacaaactag atctctcaga ctggcaagca ttcaataagt tagcgacgag gtgtaatgct
    48661 tacgatggaa taacaatgaa tggtgataat tcttttggat ggaaccatga agctacatta
    48721 gataatattc acgcccagaa atacaataaa gcttacacgg gtgcgaggtt aaccgcagaa
    48781 ctgaaatatt tattgcaaga tgtcgaatcc ttcgaaccca atagtaaata cacaatacac
    48841 gaagtggttt tgggacctgg ttatggtact ccagactata ccggccaaac gataggatac
    48901 gttgtaaccc ttcctgccca aatgcctaat tgctggtcat ctgaactacc aacaatcgat
    48961 ctttatatcg accaacttcg cactgttaca ggcgtttcta atgccctcgg attcatcatt
    49021 gctgcattgc taaacgcgta ttctgattta ccccatgatt tgaaaattgg tctgcgttcc
    49081 ttatcaagtt cagcagcaat ctactcgggt ttgggtttcg agcgagtgcc gcaggaccga
    49141 gacataagga gtgatcgtat gcatctaacg ccagcaaacc atcctgactt atggacacaa
    49201 gagaatggtg aatggatcta cttgcgaaac taatacctga cgaaaaatag aaagcaaaac
    49261 gccgaccaat ggtcggcgtt tttacgtctc gttgaataac gaattattca gctactacgt
    49321 ttacgatcac tttcgcgaat acttcgctgt gaacctggaa gctcacttcg tgttcgccag
    49381 tggtacgcag aacgccgttc ggcagacgaa cttcgctctt agccacttca acgccagctg
    49441 cagttacagc gtcagcgatg tcgcgagtac cgatggaacc gaacagttta ccttcgtcgc
    49501 cagctttaga cgcgatggta acagtttcca gtgcattgat tttctcagcg cgagcattag
    49561 cagctgccag aacttcagcc agtttagctt ccagttcagc gcgacgtgct tcgaagaatt
    49621 caatgttttt cttggtagct ggaacagctt taccctgcgg taccaggaag ttacgagcat
    49681 agcccgcttt aacgtttacc tgatcaccca ggctacccag gtttgctact ttatcaagca
    49741 gaataacttg cattacctta tcctctcaaa gtcgtattaa tggaccgtga ccgattactg
    49801 atggcgatca gtgtacggca gcagggacag gtagcgagcg cgtttgatag cgcgagccag
    49861 ctgacgctgg tattttgcac gggtaccggt gatacggctt gggacaatct taccgctttc
    49921 ggtgatgtag tttttcagcg tagcgatatc tttatagtcg atctcttgaa cgccttccgc
    49981 ggtgaaacgg cagaacttgc gacgacggaa ataacgtgcc atatggctag tctccagaat
    50041 ctatcaattc aatctgctcg gcatgcaaaa ccattttgct cagtccgttc tttgccttgt
    50101 ggcatgaaat gaacccctga acggttatgc gactgccgac cgttatactg tgagtaatgg
    50161 cctggttttc gtgtccgcta acaataacgg gcatttgaca ccatgcctgc cggtgaaagc
    50221 cggcttcctc ctgcacagaa cgatgctcaa gcacgaactg gcagtgagga attcctgatg
    50281 gactgacctt tcgaagggga gtcctgcaca cggtgccgga caacaccaga cggttggtca
    50341 tcagaaatta ctcttcagaa tccccagctt cagcatcatc agcggtttcg tttgcgaaat
    50401 catcgcgacg ctcacggcgc tcgtctttcg ctttaaccat cggagatgct tcggtaacag
    50461 cgtgcttggt acgcataacc atgctgcgga taacggcatc gttgaagcgg aaggtagttt
    50521 ccagctcatc gatcacttcc tgcggagctt caacgttcat cagaacgtag tgtgctttgt
    50581 gcagtttgtt gatcgggtaa gccagctgac ggcggcccca gtcttccaga cggtggatct
    50641 tgccttctgc accagtgatg gcagcagtgt agcgctcgat catgcccgga acctgttcgc
    50701 tctggtcagg atggaccata aaaacgattt cgtaatgacg catcgaattg ctccttacgg
    50761 attattcagc ctcctgtctg ggtcagccga atcccgggga ggcaaggaac gtgttaaagg
    50821 tcggctgaaa aatgacgcgt tatagtactt gtatgcctcg ctaaactcaa gggcagagcc
    50881 gaaataaatc tcccctttcg caattaacct ctaaaccgct gatttggagt tcatcacact
    50941 tcatcatggt gttttttcga acaactgcat cagaaatggt caccatcaac ttctttatca
    51001 ttgattaaaa ttaaatcaga agatcatcac agaggagagc acacaaagtc agccgtcgag
    51061 gaggtatcta tgttcagtcg agttttagcc cttctggctg tgcttttgct aagtgcaaat
    51121 acatgggcag ccattgaaat taataaccac caggccagaa atatggacga tgtgcaaagc
    51181 ttaggcgtga tttatatcaa ccataacttc gccactgaaa gtgaagcacg tcaggcatta
    51241 aatgaagaga cagatgcgca aggcgcaacg tactaccacg taattctgat gcgggaaccg
    51301 gggagtaacg gcaatatgca cgccagcgcg gatatttatc gctagcacca ggtataacca
    51361 acgaaacatt gccatagttt gctttgcccc cttcgcaggg ggcttttttt gaaaaacggc
    51421 tgcggaatta gaccagttat ctcccgagga aggaaatttc cgcagcgcgt gttctacttc
    51481 tgcccgtaat aagcgttagg accgtgttta cgcatgaagt gtttattcat caggaagctg
    51541 tcgatgtgat tgagttgtgg gttaatactg cgcgcaatcc acgccatttt cgccacttct
    51601 tccatcacca ccgcgttatg caccgcatcg tgggcatctt tcccccaggc gaacggtccg
    51661 tgctgataca ccacaattcc cggcgtatgc agcggctcgg cgttgcccag cgtttcgata
    51721 atcactttgc cggtgttcag ttcatactcg ccctgcacct cttcttcgct taacccgcgc
    51781 gtacacggaa tgtcgccaaa gaagtagtca gcgtgcgtgg tgcctaacgc cgggatcgcc
    51841 agccccgcct gcgcccatgc ggtggcatga gtggagtggg tatggacaat gccgccaagc
    51901 gacgggtaac gacggtagag ttcgagatgc gtcgcagtgt cggaagatgg gcgatactcc
    51961 ccttccacca ccttgccgct catatcaacc accaccatat cggccgcttt catggtttcg
    52021 taggcaacgc cgctgggttt gatcaccacc agcccgcgtt cgcggtcgat agcgctgacg
    52081 ttgccccagg taaaggtcac cagcccatag cgcggcagat ccatgttggc ttcaaacacc
    52141 tgctgtttta gcttttgcat tatgccgcct ccaccatgcc tgctttggcc atgcgcgctt
    52201 tcacccaatc gcgcgctttc gccacttctg ccgccgggtc ttccgccgtt tcgctccaca
    52261 tctcaatcag gtacggcccg caatagccac tctgtttgag cgtttcgaaa caacgttcga
    52321 aatccactac accttcgcca aacggcacgt ttttgaagac gccaggtttg gtgtctttca
    52381 catgtaccgc gacgatatgc ccgattccgg cctgcaactc catctgcaca tcgttgtccc
    52441 acgccgacag gttgccgata tccgggtaga gctggaacca cggattgttg agatagtgcg
    52501 cgtagcccag cgccttgctg atggagttca tcaacggata atccatgatc tccatcgcca
    52561 gcgtcacctg cgcgcggctt gccatctcaa cgctctcttt caggccgtca cggaaacgac
    52621 gacgcgtttc gttattggct tcctgatagt aaacgtcata gcccgccagc tggatcacgc
    52681 gaataccgac atcctgggcg aactggatag ctttacgcat aatctccagc ccctgcgccc
    52741 gcaccgcgtc atcttcactg cccagcggga aacgacgatg agcagaaagg cacatggacg
    52801 gcacgcgcac gccggtttca acaatcgcat tgaccagcgc cagacgctgc tcgcggctcc
    52861 agtcgaggcg cgacaggcgc tcgtccgttt catctaccga catctcgaca aaatcgaagc
    52921 ccaacgtttt tgccagttgc aggcgttcca gccagcactc cccggcgggg agcgcttttt
    52981 catagatgcc aagcgggatt tgtttggaca acatatccgc tccttagccc cacagttcag
    53041 cgatggaacg tttgaactga cgtgcggctt ccaccggaga agcggcatca cggatactac
    53101 ggcccgcgat aaagacgtga atcggaatac ccttgaacag cggcagatct tccagcgcca
    53161 ggcctccggt gacggtgact ttgaagccca tatcggaaag acgtttgatc gcggtgatgt
    53221 ccgcttcgcc ccacgccacg cctgcggcct gcgcgtcacg gctgcggtga taaaccacct
    53281 gctgaatgcc tgcatcgcgc cactgttgcg cctgttccca ggtccagtaa ccggtcagtt
    53341 cgatctgcac gtcgccgtta aactcttttg ccacgtccag cgcgcctttg gcggtgttga
    53401 tatccgcaca gcaaattacc gtcacccagt cagcgttggc ttcgaagcac atacgcgaaa
    53461 ggattttgcc tgcatcggca attttggcgt ctgccagtac gattttgtgc gggtagagcg
    53521 ctttcaggtc acgaaccgca cgcacgcctt cgcccacgca cagaatggtg cccacttcga
    53581 taatgtcgac ttcttcggca atcaggcgag tggtttcgta ggcgctatcc atagtctggt
    53641 tgtccagcgc gacttgcaac atcggtaatg acatgttcaa ttccttctta agctgccgcg
    53701 ttggtgcggt caatgagatc cagtacttcc tgctcggtac ggcaggcgcg taaacggtca
    53761 aaattctctt catcttcaaa caggttgacg atctgcatga tgcccacttc ctggtgagta
    53821 ttggcatcga ccgccgccat ggtgatgagg atatccaccg gatcgttgtc gtcgtggtta
    53881 aattccagcg gctttttcag cgttaccaga gagaaacctg ttttcttaac accctcttcc
    53941 ggccgcccgt gcggcatcgc cagccccgga gcgataacga aataagggcc aaactgttcc
    54001 acgccatcca gaatcgcctg gtaatagcgc ggctcgacaa catccgccgc caccagcagg
    54061 tcaacgccga ttttcactgc gtcctgccat gtctctgctt cagcctgcag gcggatggat
    54121 ttattttccg ccagcgaatc acgtaatttc atggcgcgtc cttacttcac atcctgcggg
    54181 aaatgctctt tgatcacttc cagcagtttc gggccaaagt cagcaggaga gagcatgttg
    54241 cgcacgccaa ccacgtattt gttgccggtc acggtgattt cgcccgcaat gtgcgtagaa
    54301 gcgatgatga tatccgcgcc gctcaactcg cttttgtatt cgccaaccgc gcagctgttt
    54361 accgtgtggt caatgtttga ttgggttaaa aactggtcca ctttcatctt catgatcatg
    54421 gaactgcctt gtccgttgcc acacacagcc agaatacgta cggtcataat caaaactcct
    54481 tattaagcag actgttctgc cagttgtttt tctgcatctt cttctgcgcg cagcgcgcgg
    54541 ccagcgaaga acatataagc cagtgcaatt acaatgatga cggccataaa ggcgataccg
    54601 atggagaaga agccttgcat catcggcggt gccagaatcg accagtccgc catgcccatc
    54661 caggcactca taccggtgag tttcaccgcc cagacgcaac caaagatttc gatcatcccc
    54721 atcaccagac aaatcttcag cgccgcacgc cagccgccga agtggttagc gaacacgccg
    54781 atggtggcgt tggagaagaa catcgggata aagccgggaa taatcaggat cgaggaaccg
    54841 caggcgacca ggatgccaac cgcaatcagc tgaccgatgg tgccccacat aaagccccaa
    54901 accacggcgt tcggcgcgaa gctatagata gccgcacagt caatctccag aaccgcacca
    54961 gggatcaggc gctgggagat accgttaaat gcttcagaga gttccgccac aaacatgcgc
    55021 acgccctgag tgatgatgaa gatcgccacc gcgaaggaga aaccagtttg caggatgtac
    55081 accgtccagt tcactttgcc tgccatcgcc tgcacagtgt cgataccgaa ggagagcaga
    55141 atggcaccaa agaagatggt catcacaatc gccgtggaga cgatgttgtc gtggaaaata
    55201 ttcagccagc ctggcagttt gaggtcttca acgctttcct cttttttgcc gaggaacggc
    55261 gcaactttat aggcaatcca tgatgcaaac tgttgctggt gaccgatgga gaaaccgcag
    55321 ccgtccgtca cttcctgagt cggcttgtac atcatgttgg aggtaatgcc ccagtagagc
    55381 gaaaccagaa tcgccgtgca gataatggtt gtccacatgg agtagccgaa aataaacaac
    55441 gtaacggcaa tcagccccgc ctgctggaac atgatatgac cagtcagcat gatggtacga
    55501 ataccggtaa tgcgacgcag cagcacgtaa cagatgttca gcgccagcgc taacaacacc
    55561 gcgtaaccta cccagctata ggcatcgccc atgcggtcga tggttgtcat catcgacgcg
    55621 taagtatcgg aaattgcgcc gttaatgccg tagacttcgg acattttcgc caccaccggt
    55681 ttgaaggtgc tggtgaggat gccggaccct gcctgcaaca acatgaaacc aattatggtt
    55741 ttaatcgtgc ctttaataat aacgctgaca cttttgcgca gtaggatgta gcccagacag
    55801 gtcacaatac ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa cacggtaaag
    55861 atgttgtaga ggatctccat aacgatctcc tgaagaagag gtaaccgggt attccgcaca
    55921 cccggaatgt tgtgcactca ctctaatttt caaaagtaat cacaacaaga ttatttatga
    55981 ttaaatgtga cgcgacccgc aaataattcc acacggatag agtgggatt
//
LOCUS       DAZFRE010000021        55275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFRE010000021 DAZFRE010000000
VERSION     DAZFRE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.021"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..172
                     /locus_tag="ACHKUI_001511"
     CDS             <1..172
                     /locus_tag="ACHKUI_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO6597735.1"
                     /translation="KFKYRNREFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLS
                     IPYPGSPFTGRK"
     gene            complement(309..824)
                     /gene="luxS"
                     /locus_tag="ACHKUI_001512"
     CDS             complement(309..824)
                     /gene="luxS"
                     /locus_tag="ACHKUI_001512"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6597736.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(974..2530)
                     /gene="gshA"
                     /locus_tag="ACHKUI_001513"
     CDS             complement(974..2530)
                     /gene="gshA"
                     /locus_tag="ACHKUI_001513"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6597737.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKKKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(2603..3031)
                     /gene="yqaA"
                     /locus_tag="ACHKUI_001514"
     CDS             complement(2603..3031)
                     /gene="yqaA"
                     /locus_tag="ACHKUI_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO6597738.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(3028..3594)
                     /gene="yqaB"
                     /locus_tag="ACHKUI_001515"
     CDS             complement(3028..3594)
                     /gene="yqaB"
                     /locus_tag="ACHKUI_001515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6597739.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(3875..3951)
                     /locus_tag="ACHKUI_001516"
     tRNA            complement(3875..3951)
                     /locus_tag="ACHKUI_001516"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3915..3917),aa:Arg,seq:acg)
     gene            complement(4015..4091)
                     /locus_tag="ACHKUI_001517"
     tRNA            complement(4015..4091)
                     /locus_tag="ACHKUI_001517"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4055..4057),aa:Arg,seq:acg)
     gene            complement(4156..4232)
                     /locus_tag="ACHKUI_001518"
     tRNA            complement(4156..4232)
                     /locus_tag="ACHKUI_001518"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4196..4198),aa:Arg,seq:acg)
     gene            complement(4236..4328)
                     /locus_tag="ACHKUI_001519"
     tRNA            complement(4236..4328)
                     /locus_tag="ACHKUI_001519"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4292..4294),aa:Ser,seq:gct)
     gene            complement(4644..4829)
                     /gene="csrA"
                     /locus_tag="ACHKUI_001520"
     CDS             complement(4644..4829)
                     /gene="csrA"
                     /locus_tag="ACHKUI_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6597740.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(5064..7694)
                     /gene="alaS"
                     /locus_tag="ACHKUI_001521"
     CDS             complement(5064..7694)
                     /gene="alaS"
                     /locus_tag="ACHKUI_001521"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6597741.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGSCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(7823..8323)
                     /gene="recX"
                     /locus_tag="ACHKUI_001522"
     CDS             complement(7823..8323)
                     /gene="recX"
                     /locus_tag="ACHKUI_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6597742.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(8392..9453)
                     /gene="recA"
                     /locus_tag="ACHKUI_001523"
     CDS             complement(8392..9453)
                     /gene="recA"
                     /locus_tag="ACHKUI_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6597743.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(9533..10030)
                     /gene="pncC"
                     /locus_tag="ACHKUI_001524"
     CDS             complement(9533..10030)
                     /gene="pncC"
                     /locus_tag="ACHKUI_001524"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6597744.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(10175..11260)
                     /gene="mltB"
                     /locus_tag="ACHKUI_001525"
     CDS             complement(10175..11260)
                     /gene="mltB"
                     /locus_tag="ACHKUI_001525"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6597745.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            11516..12079
                     /gene="srlA"
                     /locus_tag="ACHKUI_001526"
                     /gene_synonym="gutA"
     CDS             11516..12079
                     /gene="srlA"
                     /locus_tag="ACHKUI_001526"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO6597746.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            12076..13035
                     /gene="srlE"
                     /locus_tag="ACHKUI_001527"
                     /gene_synonym="gutE"
     CDS             12076..13035
                     /gene="srlE"
                     /locus_tag="ACHKUI_001527"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO6597747.1"
                     /translation="MIRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGNWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            13046..13417
                     /gene="srlB"
                     /locus_tag="ACHKUI_001528"
                     /gene_synonym="gutB"
     CDS             13046..13417
                     /gene="srlB"
                     /locus_tag="ACHKUI_001528"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6597748.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            13421..14200
                     /gene="srlD"
                     /locus_tag="ACHKUI_001529"
     CDS             13421..14200
                     /gene="srlD"
                     /locus_tag="ACHKUI_001529"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6597749.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            14307..14666
                     /gene="gutM"
                     /locus_tag="ACHKUI_001530"
     CDS             14307..14666
                     /gene="gutM"
                     /locus_tag="ACHKUI_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6597750.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            14733..15506
                     /gene="srlR"
                     /locus_tag="ACHKUI_001531"
                     /gene_synonym="gutR"
     CDS             14733..15506
                     /gene="srlR"
                     /locus_tag="ACHKUI_001531"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6597751.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            15499..16464
                     /gene="gutQ"
                     /locus_tag="ACHKUI_001532"
     CDS             15499..16464
                     /gene="gutQ"
                     /locus_tag="ACHKUI_001532"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6597752.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(16461..17975)
                     /gene="norR"
                     /locus_tag="ACHKUI_001533"
     CDS             complement(16461..17975)
                     /gene="norR"
                     /locus_tag="ACHKUI_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6597753.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFLRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            18162..19601
                     /gene="norV"
                     /locus_tag="ACHKUI_001534"
     CDS             18162..19601
                     /gene="norV"
                     /locus_tag="ACHKUI_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6597754.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            19598..20731
                     /gene="norW"
                     /locus_tag="ACHKUI_001535"
     CDS             19598..20731
                     /gene="norW"
                     /locus_tag="ACHKUI_001535"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6597755.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIIGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(20955..23207)
                     /gene="hypF"
                     /locus_tag="ACHKUI_001536"
     CDS             complement(20955..23207)
                     /gene="hypF"
                     /locus_tag="ACHKUI_001536"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6597756.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVAILRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFSLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDVAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAAPCHRVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLVHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(23360..23887)
                     /gene="hydN"
                     /locus_tag="ACHKUI_001537"
     CDS             complement(23360..23887)
                     /gene="hydN"
                     /locus_tag="ACHKUI_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6597757.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(24036..25046)
                     /gene="ascG"
                     /locus_tag="ACHKUI_001538"
     CDS             complement(24036..25046)
                     /gene="ascG"
                     /locus_tag="ACHKUI_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6597758.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINDGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            25306..26763
                     /gene="ascF"
                     /locus_tag="ACHKUI_001539"
     CDS             25306..26763
                     /gene="ascF"
                     /locus_tag="ACHKUI_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6597759.1"
                     /translation="MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFTLIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            26772..28196
                     /gene="ascB"
                     /locus_tag="ACHKUI_001540"
     CDS             26772..28196
                     /gene="ascB"
                     /locus_tag="ACHKUI_001540"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6597760.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQMKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMNANNCSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(28294..28713)
                     /locus_tag="ACHKUI_001541"
     CDS             complement(28294..28713)
                     /locus_tag="ACHKUI_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6597761.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(28784..29539)
                     /locus_tag="ACHKUI_001542"
     CDS             complement(28784..29539)
                     /locus_tag="ACHKUI_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597762.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSGSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDSFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQSEDLVLTQRCLIKGDVLDGVNQL"
     gene            complement(29582..30052)
                     /gene="hycI"
                     /locus_tag="ACHKUI_001543"
     CDS             complement(29582..30052)
                     /gene="hycI"
                     /locus_tag="ACHKUI_001543"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6597763.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE"
     gene            complement(30045..30455)
                     /gene="hycH"
                     /locus_tag="ACHKUI_001544"
     CDS             complement(30045..30455)
                     /gene="hycH"
                     /locus_tag="ACHKUI_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6597764.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(30452..31219)
                     /gene="hycG"
                     /locus_tag="ACHKUI_001545"
     CDS             complement(30452..31219)
                     /gene="hycG"
                     /locus_tag="ACHKUI_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6597765.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(31219..31761)
                     /gene="hycF"
                     /locus_tag="ACHKUI_001546"
     CDS             complement(31219..31761)
                     /gene="hycF"
                     /locus_tag="ACHKUI_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6597766.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(31771..33480)
                     /gene="hycE"
                     /locus_tag="ACHKUI_001547"
     CDS             complement(31771..33480)
                     /gene="hycE"
                     /locus_tag="ACHKUI_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6597767.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(33498..34421)
                     /gene="hycD"
                     /locus_tag="ACHKUI_001548"
     CDS             complement(33498..34421)
                     /gene="hycD"
                     /locus_tag="ACHKUI_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6597768.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(34424..36250)
                     /gene="hycC"
                     /locus_tag="ACHKUI_001549"
     CDS             complement(34424..36250)
                     /gene="hycC"
                     /locus_tag="ACHKUI_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6597769.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(36247..36858)
                     /gene="hycB"
                     /locus_tag="ACHKUI_001550"
     CDS             complement(36247..36858)
                     /gene="hycB"
                     /locus_tag="ACHKUI_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6597770.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(36983..37444)
                     /gene="hycA"
                     /locus_tag="ACHKUI_001551"
     CDS             complement(36983..37444)
                     /gene="hycA"
                     /locus_tag="ACHKUI_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6597771.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            37656..38006
                     /gene="hypA"
                     /locus_tag="ACHKUI_001552"
     CDS             37656..38006
                     /gene="hypA"
                     /locus_tag="ACHKUI_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6597772.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            38010..38882
                     /gene="hypB"
                     /locus_tag="ACHKUI_001553"
     CDS             38010..38882
                     /gene="hypB"
                     /locus_tag="ACHKUI_001553"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6597773.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            38873..39145
                     /gene="hypC"
                     /locus_tag="ACHKUI_001554"
     CDS             38873..39145
                     /gene="hypC"
                     /locus_tag="ACHKUI_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6597774.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            39145..40266
                     /gene="hypD"
                     /locus_tag="ACHKUI_001555"
     CDS             39145..40266
                     /gene="hypD"
                     /locus_tag="ACHKUI_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6597775.1"
                     /translation="MRFVDEYREPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASAF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            40263..41273
                     /gene="hypE"
                     /locus_tag="ACHKUI_001556"
     CDS             40263..41273
                     /gene="hypE"
                     /locus_tag="ACHKUI_001556"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6597776.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            41347..43425
                     /gene="flhA"
                     /locus_tag="ACHKUI_001557"
     CDS             41347..43425
                     /gene="flhA"
                     /locus_tag="ACHKUI_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6597777.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            43470..43898
                     /locus_tag="ACHKUI_001558"
     CDS             43470..43898
                     /locus_tag="ACHKUI_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO6597778.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRIIALIKASAILIATKA"
     gene            complement(43937..44281)
                     /gene="ygbA"
                     /locus_tag="ACHKUI_001559"
     CDS             complement(43937..44281)
                     /gene="ygbA"
                     /locus_tag="ACHKUI_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6597779.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            44567..47128
                     /gene="mutS"
                     /locus_tag="ACHKUI_001560"
     CDS             44567..47128
                     /gene="mutS"
                     /locus_tag="ACHKUI_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6597780.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQHRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            47234..47890
                     /gene="pphB"
                     /locus_tag="ACHKUI_001561"
     CDS             47234..47890
                     /gene="pphB"
                     /locus_tag="ACHKUI_001561"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6597781.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAENELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(47941..48708)
                     /gene="ygbI"
                     /locus_tag="ACHKUI_001562"
     CDS             complement(47941..48708)
                     /gene="ygbI"
                     /locus_tag="ACHKUI_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO6597782.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            48904..49812
                     /gene="ltnD"
                     /locus_tag="ACHKUI_001563"
     CDS             48904..49812
                     /gene="ltnD"
                     /locus_tag="ACHKUI_001563"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO6597783.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            49809..51071
                     /gene="otnK"
                     /locus_tag="ACHKUI_001564"
     CDS             49809..51071
                     /gene="otnK"
                     /locus_tag="ACHKUI_001564"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO6597784.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTEILAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            51068..51706
                     /gene="ygbL"
                     /locus_tag="ACHKUI_001565"
     CDS             51068..51706
                     /gene="ygbL"
                     /locus_tag="ACHKUI_001565"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6597785.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            51711..52487
                     /gene="ygbM"
                     /locus_tag="ACHKUI_001566"
     CDS             51711..52487
                     /gene="ygbM"
                     /locus_tag="ACHKUI_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6597786.1"
                     /translation="MPRFAANLSMMFIEVPFIERFAAARKAGFDAVEFLFPYDYSTQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHAQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            52576..53940
                     /gene="ygbN"
                     /locus_tag="ACHKUI_001567"
     CDS             52576..53940
                     /gene="ygbN"
                     /locus_tag="ACHKUI_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO6597787.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(54083..55126)
                     /locus_tag="ACHKUI_001568"
     CDS             complement(54083..55126)
                     /locus_tag="ACHKUI_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO6597788.1"
                     /translation="MQGIKIYTILASSLISGSVFAQTEISTANSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
BASE COUNT        13232 a        14889 c        14225 g        12929 t
ORIGIN      
        1 gaaattcaaa tacaggaaca gggagttctg gtgcagaggg tattacgtcg atacggtggg
       61 taagaacacg gcgaagatac aggattacat aaagcaccag cttgaagagg ataaaatggg
      121 agagcagtta tcgattccct atccgggcag cccgtttacg ggccgtaagt aacgaagttg
      181 gatgcaaatg tcagatcgtg tgcgcctgtt agggcgcggc tggtaagaga gccttatagg
      241 cgcatttgaa aaacctccgg ctatgccgga ggatatttat tcaattaatt gtgaagatag
      301 tttactgact aaatgtgcag ttcctgcaac ttctctttcg gcagcgccag ttcttcgttg
      361 ctgttgatgc gcacgtcacg ttccagaatg ttacgcgcaa tatcctgcgc ttcctgcaac
      421 gagtgcatct ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc
      481 tgcactttca gcacgtcttc cattgccgct ttccaggcat cagcgacacg ctgctcatct
      541 ggcgtaccaa tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata
      601 atctctacgc cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg
      661 gtatggatcc ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac
      721 acggtgattg cgtcgccatg cggggtgttc attgtttttg ccacccgaac tgcaggcgct
      781 tccatccggg tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta
      841 atttttttaa aaattttctg aactctttgt tcccaggcga gtctgagtat atgaaagacg
      901 cgcatttgtt atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc
      961 tttttctttt ctgtcaggcg tgcttttcca gccacaccgc aaacggttcg gtatcagcgg
     1021 cttccatttc ctgctgacgg cgttcagacg cctcgcgctc ggcgacaaaa tcctcttcgc
     1081 gcagaatttc cagcggctct tcacgcagca gattacggta cgcttcagca aacgctttgc
     1141 ctgttccgcc aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat
     1201 caggattatc gaagcaggcg accagttcat cacacacttt ctgatacgct tcgccgccgt
     1261 taatgctatc cagcgtttgc gcgacgcgtt tcagatcgcg gaacagatct ttacccacct
     1321 gcggtaacgg gaactgcgca gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac
     1381 cttcgaggat cacccggttc cagttaacgc gtgtacaggc aagttcgcta ctgctcattt
     1441 ccggtgcatc agccagcgca caccagacca taaacaggtc gaggaatcgc acctgttgtt
     1501 catctacacc aatcggcgag aacgggttga tgtccagcga gcggacttca atatattcaa
     1561 tgccgccacg taacagcgca tcagaaggcg actcgccgct gcgggtaacg cgttttggac
     1621 gaatcggcgc gtacagttcg ttttcaatct gcagcacgtt gctgttgatt tgcagccgct
     1681 taccgtcttt ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt
     1741 taaggcccgc cacgtactcg tagagatcat tgaaggtgat accaagattg ctttgcgatt
     1801 tattggtata gccaagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac
     1861 cgcactcggt tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa
     1921 tcgctggaga cgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga
     1981 taacgcggaa atagcccgca gaaattttct ttttggcatc agcgcccgag atatcaccgc
     2041 acttcgcttg ccagaatgcc attggcaaag agaaattata gtgcacgccg gaaatggttt
     2101 gcatcagcgc gccgtagcga tttttcagcc cttcacggta cagcgtttta aagcgaccgg
     2161 tgttagaagt gccgtactgt gccagttcga tgtcctgacc ttctgcgata tagcatggca
     2221 tacttaacgg ccacatccgc tcatcgccca tattgcgcgc cgtatagcga tgcagatcgc
     2281 gcataaaggt cagcatatgt tcaatatcac catccactgg tgtaataaat tccagcaatg
     2341 cttccgcaaa atcggtagta atccatttat gggtcagtgc agaacctaat gcttcaggat
     2401 gacctgttgt tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac
     2461 gctgtatccc ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt
     2521 ccgggatcaa attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt
     2581 gaccatatgt gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg
     2641 caactgcaac atagcgtaac gctttaccaa ggcacaaaaa aaagattacc ggtccccacg
     2701 agatgcgcat ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta
     2761 ataatagcgt gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg
     2821 atgttttacg caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat
     2881 tacccattgt tgctgttaaa actaaaaccc agggatgact cactccggaa agcaacattg
     2941 ccaccagcac gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa
     3001 acaacgagaa aagcgataac gcttcactca cagcaagcga acatccacgg cgtccatgcc
     3061 tgctgcacgg gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg
     3121 ttgcacgccc atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt
     3181 gacgtgatcg gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gtgccagcaa
     3241 tgcctcagcg atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca
     3301 gcttttcacc acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac
     3361 tgcttctgtt ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat
     3421 aatcgcctga gcaatacgcc aggtgggcga gccattaagc gcaatcatcg cctgaacatc
     3481 gtactgaaga ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctcggt
     3541 atccaggatg gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt
     3601 ctcgcaagta gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg
     3661 agaaggagaa gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg
     3721 aaggaaattt ttttgagaag atggtgcatc cgggaggatg actcactggc gttcgccctt
     3781 cgggccgctg ctggcgcaac gttatcctcc ctggtgcctg cggttaatac cgctacctct
     3841 tcgatacctt cattgctgaa ggttacggag aatatggtgc atccgggagg attcgaacct
     3901 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa
     3961 atttatttta ctgctgatat tgatatcgcc actaaagcca tatcaagtaa gagatggtgc
     4021 atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga
     4081 gctacggatg catcgggaaa cttactttac tgcagatttt ttgataccgc cactaaagtc
     4141 gtatcaagta agagatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
     4201 ccgagtactc tatccagctg agctacggat gcaaatggcg gtgaggcggg gattcgaacc
     4261 ccggatgcag cttttgaccg catactccct tagcagggga gcgccttcag cctctcggcc
     4321 acctcaccac acgcctctta cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg
     4381 cgtggcgcac atattacttt ctgagactta taagtcaaac aatttttccc acacttttat
     4441 cgtttgcaca cttcatgctc aattagtctg taaaaacgtc aaaaagagtg ttttatcaac
     4501 agaaaaacgg aggtctgaca gatagcagta atgcaaaaaa atggagactt aagttgaatg
     4561 aacgggagta aagcgaaaag acaatggagt gaaagagaaa ttttgagggt gcgtctcacc
     4621 gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc agcctggata
     4681 cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac gccaatacgt
     4741 acctggttgc cctttacccc taaaactgtc acggtgacct catccccaat catgagggtc
     4801 tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta aaagagtcgg
     4861 gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca atgacaaaca
     4921 cattacatct aagcagtcat ggcattacat tctgttaaac ctaagtttag ccgatataca
     4981 caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc
     5041 cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc tttcacactg
     5101 gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt
     5161 ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc tgctttcaca
     5221 cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc ggctaccgtt
     5281 gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat ggtacgcaac
     5341 attttcggct caacaccgct aagctcgcta accaacagct taacaccatt aacatcaatt
     5401 gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag ctgttgtaac
     5461 tctttttcca gctgacgcgt acgttccagt actgaacgca ctttatcagc cagattattg
     5521 ctatcgcctt tcagcagatg cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg
     5581 gcgatagcgc cttctccggt caccgcttcg atacgacgaa cgcctgcagc agtacccgat
     5641 tcagagatga tgcggaacag accaatatca ccagtgcggc tggcgtgagt accgccacac
     5701 agctcggtgg agaaatcgcc catgctcagc acgcgtacgc gctcatcata cttctcgcca
     5761 aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc
     5821 ggcaagttac ggcgaatctg tgcgttcacc aggtcttcga ccgcacgaat ctcttctggt
     5881 ttcatcgctt cgttgtgtga gaagtcgaag cgcagcactt tgtcgttaac cagtgaacct
     5941 ttctgcgata catgagtacc cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg
     6001 gagtgattca gacgaatacg ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg
     6061 cccactttca gagaacccgc agcaagttta ccgatgtggc caatcgcctg gccgtatttc
     6121 tgcgtatctt ccaccgcgaa ggagaagtta gcgcctttca gttcgccttt atcaccaacc
     6181 tgaccgccgg attccgcata gaatggcgtt tgatccagca cgaccacagc ttcctggcct
     6241 gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt gccgttcagt
     6301 tccagatggt catagccttt aaattcagat gcactgtcaa cacggatcat tgcgttgtaa
     6361 tcggcaccaa agccgctggc ttcgcgcgca cgacgacgct gctcttccat tgctgcttca
     6421 aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt caggtcaacc
     6481 gggaagccgt aggtgtcgta caggcggaaa gcagtttcac catccagcgt atcaccagaa
     6541 agttttgcca gctcttcatc cagcagcgcc agaccgcgct ccagagtacg ggcaaactgc
     6601 tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc
     6661 gcagagccca taacgtcgat cagcggacca accagtttgt agaagaaggt ttctttcgcg
     6721 ccgagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac ataaccacgg
     6781 ttttcgttgg acggcattac gccatccgcg atcaggaacg cacaagaacg aatgtggtca
     6841 gcgattacgc gcagcgattt attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc
     6901 tggatcaacg tgcggaacag gtcgatgtca tagttagagt taacgtgttg cagcaccgca
     6961 gcaatacgct ccagacccat accggtatct acagacggtt tcggcagcgg ttccatcgtg
     7021 ccatcggcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat gtagcggtcg
     7081 ccgtcttctt ccggacttcc cggagggccg ccccaaatgt ggtcgccgtg atcgtagaag
     7141 atttcggtgc acgagccgca cggaccagtg tcacccatct gccagaagtt gtcagatgcg
     7201 tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat ccctacttct
     7261 ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca cagacgctct
     7321 ttcggcaggg caaaccattt ttcgctggtc agcagttccc atgcaaactg aatggcatcg
     7381 tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt atggtgacgc
     7441 gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg
     7501 gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc cttgaactgg
     7561 ttcatcccgg cgttggtaaa caacagagtt gggtcgttat ggggtaccag ggagctgctg
     7621 gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg gatctcagcg
     7681 gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgctac cagccgttgc
     7741 gtgtcgttaa ccgcccggct ggtaactgaa aagtgggaat aagataagtt ttcttgactg
     7801 ggaagtaaaa tcccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt
     7861 ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag
     7921 ttggcaaagg ttcgccatat tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga
     7981 tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt
     8041 cctgacgaat acgcgcaggt ccataacctt tgcggctacg gctggcgata aagcgcgcaa
     8101 caaatcggct gtcatcaaga tagccatgtt catggcacca ggcaataacg cgctcgtaat
     8161 cttctgccgt ggcatcaatc tcttccgggc catttttgcc cataatcggt gccgcgagtt
     8221 tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca
     8281 acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat ccttacaact
     8341 taaaaaagca aaagggccgc agatgcgacc cttgtgtatc aaacaagacg attaaaaatc
     8401 ttcgttagtt tctgctacgc cttcgctgtc atctacagag aaatccggcg ttgagttcgg
     8461 gttgctcagc agcaactcac gtactttctt ctcgatctct ttcgcggttt ctgggttatc
     8521 tttcagccag gcagtcgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta
     8581 ccacgcgcct gctttctcga taagcttctc tttcacgccc aggtcaacca gctcgccgta
     8641 gaagttgata ccttcgccgt agaggatctg gaattcagcc tgtttaaacg gcgcagcgat
     8701 tttgttcttc accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac
     8761 cgcgccgata cgacggatgt cgagacgaac ggaggcgtag aatttcagcg cgttaccacc
     8821 ggtagtggtt tccgggttac caaacatcac accaattttc atacggatct ggttgatgaa
     8881 gatcagcagc gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat
     8941 catacgtgcc gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg
     9001 cgtcagtgcc gccacggagt caacgacgat aacgtctact gcgccagaac gtgccagggc
     9061 gtcacagatt tccagtgcct gctcgccggt atccggctgg gagcacagca ggttgtcgat
     9121 atcgacgccc agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc
     9181 acaggtttta ccttcgcgct gcgctgcggc gatcacctgc aacgtcagcg tggttttacc
     9241 ggaagattct ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag
     9301 cgcgatatcc agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc
     9361 acccaggcgc atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc
     9421 caacgctttc tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac
     9481 cggatagtca atatgttctg ttgaagcaat tatactgtat gctcatacag tatcaagtgt
     9541 tttgtagaaa ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg
     9601 catcacggtc accgctgaag cattcccgcc gggtaatgcc ttcaccacgg gcagtggcaa
     9661 aagcaaacca gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac
     9721 cactaataga cacggcgtaa tcagcacgag ccgctttcag tgcgcctatc gccatttcca
     9781 ccacgacggg ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgc acgccgatca
     9841 tctgcgcttt ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac
     9901 cggcaatatc ggtaatcact ttcgctaccc aaccgccggt acaagactca gcagttgtta
     9961 cggttgcgcc acggactttc agcgcctgcc caacctgttc gcttaactgc atcagttcac
    10021 tgtcagtcat cacaacctct gtcagtcata aatttatgcc ggacaagata gcactttcgt
    10081 caggaagatg gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc
    10141 cagagggaag ctcacgcccc cctctggcaa atagttactg cacgcgcgcc agcgccacgg
    10201 cttgtcctaa ctgccagacc gccattgcgt aatgggtgct gtggttataa cgggtaatgg
    10261 tgtagaagtt cggcagaccg taccagtact ggtaaccggt accgacatcc agacgcagca
    10321 ggctggcttg ttgatggttg cccagcggct gctgtggcgt taaacctgcc gcggcaagct
    10381 gcgaaatgct gtacctggtt ttgaagccat ttggcaagcc tggcgcctga ccgtttgcca
    10441 ttaccgcgac ctgatcgcct ttcacccagc cgtgcgcttt gaaataattc gccacgctac
    10501 cgatcgcatc aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat
    10561 attgtttgta agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt
    10621 tcagattgag cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc
    10681 cagaaaaata ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga
    10741 tgcgagtttt ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa
    10801 tttccggcgg tacaccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc
    10861 agaaaaccac accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc
    10921 atgcgccgtt cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca
    10981 gccgcagtac cgaatccaga cgtttcgcct gggagagaat ttcctgcaac tgctgacgat
    11041 cgaaaccgtg tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattggcga
    11101 agtcaccgcc catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg
    11161 ttccggtggt cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca
    11221 caaaaagggg aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat
    11281 tcaaccaagg gcaagtatgg taaagcatcc cgccccgcac aaggaagcgg tagtcactgc
    11341 ccgatacgga ctttacataa tcaactcatt ctcctcacta ccttctttat acaaactttc
    11401 aaaataaaat atttatcttt cattttgcga tcaaaataac acttttaaat ctttcaatct
    11461 gattagatta gattgccgtt tggtaataaa acaataaata ctgaaggaga gaacaatgat
    11521 agaaaccatt actcatggcg cagagtggtt catcgggctg ttccagaaag gcggagaagt
    11581 gtttaccggg atggtaaccg gcattcttcc gctgttaatt agcctgctgg ttatcatgaa
    11641 cgcattgatt aattttatcg gtcagcatcg tattgaacga tttgctcaac gttgcgccgg
    11701 taaccctgtt tcccgttacc tgctgctacc gtgcattggt acgtttgtct tttgtaaccc
    11761 gatgacctta agtcttggtc gctttatgcc ggaaaaatac aaacccagct actacgcagc
    11821 ggcctcttat agctgccact caatgaatgg cctcttccct catatcaacc ctggcgaact
    11881 gtttgtttat cttggcattg ccagcggtct gacgacgctg aatctgccac ttggcccact
    11941 ggcggtgagc tatctgctgg ttggtctggt caccaacttc ttccgcggct gggtaaccga
    12001 tctgaccacc gccattttcg agaaaaagat gggcattcaa cttgaacaaa aagttcgtct
    12061 ggcaggagca acatcatgat acgcattcgg atcgaaaaag gaacgggtgg ctggggcggc
    12121 ccgcttgagc tggaagccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccga
    12181 cctgcgattg ttgacaaact ggcacagctt actggctggc aagctatcga cggatttaaa
    12241 gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta
    12301 cgctgcggca tctatccgaa acggcgtatt cccaccatta atatccactc gacgggcaag
    12361 tccggcccgc tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa
    12421 aacatcactg tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac
    12481 accagcaaga aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggtatg
    12541 ggttctgccg ttgccgtgct gttccagtct ggccgtgaca ccatcgacac tgtattaaaa
    12601 accattctgc cgtttatggc gttcgtctcg gcgcttatcg gaatcattat ggcttctggc
    12661 cttggtaact ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc
    12721 atgctggcgc tcatctgctc cttcccgctg ctttcacctt tcctcggccc aggcgcagtt
    12781 atcgcacagg ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg
    12841 catctggctt tacctgcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg
    12901 gtcggtttgt cgctggcgga agctcgtcag gacacggttc gcgtcggtgt cccttctgtt
    12961 ctggtgagcc gctttttaac cggcgcgcca actgtactga tcgcctggtt tgtctccggt
    13021 tttatctatc aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat
    13081 tggcgcgagt gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc
    13141 gcctgcggat ctcgaagagt attgcttcat tcattgccac ggcgagttaa aaggtgcact
    13201 ccatcccggt ttgcaatttt ctctcgggcg gcatcgctat ccggtgaccg ccgttggcag
    13261 cgtggcggaa gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaag
    13321 cgaagcggaa tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc
    13381 gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt
    13441 catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta
    13501 tcgcgtggcg gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa
    13561 cgccgaatat ggtgaaggaa cagcgtacgg ttttggtgct gacgccacca gcgagcaaag
    13621 cgttctggcg ctttctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta
    13681 cagcgccgga atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg
    13741 ttcgctacag gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt cgcgtttgat
    13801 gatccgcgac gggattcagg gtcgcattat tcagatcaac tcgaaatccg gcaaagtggg
    13861 cagcaaacac aactctggct acagcgccgc gaaatttggt ggcgtcgggc tgactcaatc
    13921 actggcgctg gatctggcgg agtacggcat tacggtgcat tcgctgatgc tcggtaacct
    13981 gctgaaatcg cctatgttcc agtcgctatt gccacaatac gcgaccaagc tgggtatcaa
    14041 accggagcaa gtcgagcagt attacatcga taaagtaccg ctcaaacgcg gctgcgatta
    14101 tcaggatgtg ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt actgcactgg
    14161 tcagtcgatc aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg agatctttta
    14221 aggatctccg cgcgactaat agaatgcctg atgcgctacg cttatcaggc ctacaggact
    14281 tccgccacta catttaagga gaagttatgg tatccgcact catcaccgtc gccgttatcg
    14341 cctggtgtgc gcaactggcc ttaggcggtt ggcaaatttc tcgttttaac cgtgccttcg
    14401 acacgctatg ccagcaaggg agggttggcg tgggccgttc cagcgggcgc tttaaaccgc
    14461 gggtcgtggt cgctattgcg ctggacgatc agcagcgcat cgtcgacacc ttgtttatga
    14521 aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggc atgcatgtgg
    14581 gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat
    14641 tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat
    14701 cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca
    14761 ttctggagta tctacaaaag cagggtaaat gctcggttga agaattggcg caatactttg
    14821 acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca
    14881 ttcgtactta tggcggcgtg gtgttgaaca aagaggaatc cgatccgcct atcgatcata
    14941 aaacgcttat caacacccac aaaaaagagc tgattgcaga agctgccgtt ggttttattc
    15001 atgatggcga ctcaataatt cttgatgctg gcagtaccgt gttgcagatg gtgcccctgc
    15061 tctcgcgctt taataacatc acggtgatga ccaacagcct gcacatcgtc aatgcgctat
    15121 ccgaactgga taacgaacaa actatactga tgccaggcgg aacatttcgc aaaaaatcgg
    15181 cctcatttca cgggcaactg gcagagaatg ccttcgagca tttcaccttc gataaattgt
    15241 ttatgggcac cgacggcatc gatctcaatg cgggcgtaac cacctttaac gaggtctata
    15301 ccgtcagtaa ggcgatgtgt aatgccgcgc gcgaagtgat tttgatggct gactcatcga
    15361 agtttggtcg taaaagcccc aacgtggttt gtagtcttga aagcgtcgat aagctgatta
    15421 ccgacgcagg tatcgatcca gcgtttcgtc aggcgctgga agaaaaaggg atcgacgtga
    15481 tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg
    15541 ctggagttgc aggaagcaag ccatttaccg gaacgtctgg gcgatgattt cgttcgcgcc
    15601 gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc
    15661 cacattggta agaaaatcgc cgcaacgctt gccagcaccg gcacaccggc tttttttgtc
    15721 catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg
    15781 tttatctctt attccggtgg cgcgaaggaa ctggatctca ttattccgcg tctggaagac
    15841 aaatctatcg ctctgctggc aatgaccgga aaaccgacgt caccgctggg cctggcggcg
    15901 aaagcggtgc tggatatctc cgtagaacgc gaagcctgtc cgatgcacct tgcgccgact
    15961 tccagcaccg tgaataccct gatgatgggc gacgcgctgg cgatggcggt catgcaggcg
    16021 cgcggattta atgaagaaga ttttgcccgc tcccacccag ccggggcact gggcgctcgc
    16081 ttgctgaaca aagtgcatca tcttatgcgt cgtgacgatg ccatcccgca ggtggcgtta
    16141 accgccagcg tgatggacgc gatgctggaa cttagccgca ccggtttagg tctggtggca
    16201 gtgtgcgatg atcaacgact ggtgaaaggc gtctttaccg acggtgattt acgtcgctgg
    16261 ctggttggcg gcggcgcact caccacgcca gtcaatgaag cgatgacggt cggcggcacc
    16321 acgttgcaat cgcaaagccg tgccatcgac gccaaagaaa tcctgatgaa acgcaaaatc
    16381 accgccgcac cggtagtgga tgaaaacggc aaactcaccg gcgcaattaa cctgcaggat
    16441 ttctatcagg ccgggattat ttaatccttc agccccagac gtttcgccag ccgatgcagg
    16501 ttggcgacgt cggtttccag catccgcgca caggcggccc agttgtgatg attttgcgcc
    16561 agtgcctgac gaatggtttc acgcaggaac gcttctgtcg cttcacgcag attttgctta
    16621 acaacgggca ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt
    16681 tgcgcctcaa gaatcacttc atcgccgctg cgggtggctc tcgccagcac taccgcccga
    16741 tgaatagcat gttccagttc acgaacgttc cccggaaaat tgtagtgttg cagtaaattt
    16801 cgcgctccgg cacttaatac cactcgtgag agccccagcc gcaaacgaca ctgctcgcag
    16861 aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg cactgaaagt
    16921 ggaaacacgc tcaggcggtg gaacaagtca gcgcgaaatc gccctgccag tacctcttcg
    16981 cgtaagtcac ggttagtcgc cgccagcacg cgcacatcga cccgcaaact gcggtcatcg
    17041 ccaacgcgtt gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac
    17101 gacaactcgc cgatctcatc gagaaacagc gtgccgttat ccgccatttc aaacttcccg
    17161 ctgcggttac tgatagcacc agtaaacgcc cctttcacat gcccgaacaa ctcactttcc
    17221 gccacacttt ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc
    17281 gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctaatt
    17341 aaaacgttga gatcggatgc cgccacaatc tcaatctctt ttttcaattg cgtcatgcct
    17401 ggcgacagac caatcatctg cgtctgtttc accgcttcaa acggcgcggc atcgcctggc
    17461 agcatattct ggctttccag ttgttcaatc agcaatgcat tgcttaacgc tcccgccgcc
    17521 agtgcggcaa tcagccgtag ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc
    17581 ccgtcgagcg tcaaggcacc gatcaggttt tgcccggcaa acaacggcaa accaacgcag
    17641 gcgtgaacct tcagactctc ctgccctgga atcaaaccgt cataaggatc gggcaattcg
    17701 ctgtctgcgg gaaagcgcac cacgtccccg gcgcgggcaa tcgcttccag ccgtggatga
    17761 ccttccagcg caaaacgtct accgagtaca tccttcgcca gcccgtcgat ggcaagcgga
    17821 ataaactgcc gcgagtcgta acgtagcaac gcagacgcat cgcactccag cacctgacgc
    17881 agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg
    17941 gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta
    18001 tagatattgt catatcgacc attttattga tagtcatttt gactactcat taatgagcac
    18061 aattttattt atagagtaaa aacaattaga taaaaaactg gcacgcaatc tgcaattagc
    18121 aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa
    18181 ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac ggtacagaat
    18241 ataaaacgct gcgcggcagc agctacaaca gctacctcat ccgcgaagaa aaaaacgtgc
    18301 tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa
    18361 tcgatctggc agatatcgat tacatcgtaa ttaaccatgc tgaagaggac cacgccgggg
    18421 cgctgaccga actgatggcg caaattcccg atacgccgat ctactgtaca gccaacgcta
    18481 tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg
    18541 gcgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaacg ccaatgctgc
    18601 actggccgga cagcatgatg acttacctga caggcgacgc ggtgctattc agtaacgatg
    18661 ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc
    18721 ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa
    18781 cgccgaaaat taccgagatc ctgggcttta acttgccagt cgatatgata gcgacctctc
    18841 acggcgtggt atggcgcgat aacccgacgc aaattgtcga gctgtacctg aaatgggcgg
    18901 cggattatca ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca
    18961 tgatggctga cgctatcgcc caggggattg cggaaaccga cccacgcgtg gcggtgaaaa
    19021 ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag
    19081 gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg
    19141 tggaggagat gaccggatta cgcttccgta acaaacgcgc cagcgctttt ggctctcacg
    19201 gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa
    19261 tgtcgcttag cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgtcgtg
    19321 aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga
    19381 atacggtggt tgaagaagaa acctctgccg ccacgacggc tgacctcggc ccacggatgc
    19441 aatgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg
    19501 cgccaggaac gccgtggagt gaagtcccgg ataacttcct gtgcccggaa tgctctctcg
    19561 gcaaagacgt ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat
    19621 tggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccagtat
    19681 tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaacctg acctcagcca
    19741 tgttatcagt caggggcaac gtgccgacga ccttacccgc cagacggcag gtgaatttgc
    19801 cgagcagttt aatctgcgcc tgtttccgca cacctgggta acggatatcg atgccgaagc
    19861 ccatgtggtg aaaagtcaga ataatcagtg gcaatacgac aagttagtgc tggcaaccgg
    19921 tgccagcgcc tttgtcccgc cagtgcccgg gcgtgagtta atgctgacgt taaatagtca
    19981 gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattatcgg
    20041 cggtggtttg attggtagcg aactggcgat ggatttttgt cgggcaggca aaatggtcac
    20101 gctaatcgac aacgctgcca gtattctggc gtcgttaatg ccaccggaag tcagcagccg
    20161 cttgcagcat cggttgacgg agatgggcgt tcatctgctg ttgaaatctc agttgcaggg
    20221 actggaaaaa acagattctg gcattctggc aacgctggac cgccagcgtt gcatcgaagt
    20281 ggatgcggta attgccgcca ccggactgcg cccggaaacc gccctggcac gacgcgccgg
    20341 gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat
    20401 ttatgcgctg ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tccagccgat
    20461 tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact
    20521 cccggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac
    20581 ccagcgtcag gatttacgct ggcaaattaa taccgaacgc cagggaatgg tggcgcgcgg
    20641 cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc
    20701 atttggattg ttgaaaacgt tgtcgatgta ggcgagctac agtgccccaa atgtcggatg
    20761 cggcgctgac gcgtcttatc cgacctacag ggaacgcatg tgttgatcac ataatacgtt
    20821 tatgtcgcat cctgcactgt cccaaaatgt cggatgcgac gctggcgcgt cttatccgac
    20881 ctgcggggat gaatatgtag gccggataag gcgttcacgc cgcatccggc aatggtgtcc
    20941 aaatgcagca cgttttatcc gttctggact tcccctgcca accaacgcgc cgcaacaata
    21001 accccctgcc ccagagacaa cccgccatcg cccgcaggta aactctgtgg aaagagcaat
    21061 gtgaaatcag cgagataatg caccagacgt gcacgcagca aacggttatg aataactccg
    21121 ccgctaaata ccagcgtagt gataccacgc atcgtggcct gctcacgcat caacgcggca
    21181 aaaccctgcg ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccgg agcctgccag
    21241 ttcagccact gctgccagaa agtggcgaga tccagttgat tgtccaccag cggcatcgtc
    21301 accggatgcg tcactctgtg gcacggagct gcgagtgcct ccagagcaca agccgcttca
    21361 ccttcataac ttaacgtggc tggcgcacag cccagcgccg ccgctacggc atcgaacaaa
    21421 cgtccacacg acgatgccag cggcgcgtta attccacgct caatagcccg cgccagcacg
    21481 ctccagtttt gctgttgcac acttgccgtt tcagagtaat tctgccactc cggcacaaag
    21541 cgcaggcact gcgccagcag gtttcgccac ggctgcttcg cagccacatc gccccccgga
    21601 agcgccaccg cgggcaagcc gcccaggtgc tggcattcac gatagttcac ccgcaagcac
    21661 tcgccccccc ataaagcgcc gttctccccc ataccaatac cgtcgagcgt caacgcaatg
    21721 acatctccgc catccagagg ccagaggtgt tctgccagac acgccgctgc gtgggcatga
    21781 tgatgcagta ccgtttgcgt cggcagattc atttcgcgcg cccactggct ggagacatag
    21841 cccggatgcg cgtcatgcac aacgtattgc ggagtgaaat cgtagatgtt ttgcatcagg
    21901 cgtaacgctt cgcgccactg catctggatg ccatcgtcac ttaaatcgcc cagatgctga
    21961 ctcaacaccg cttgttcgcc gcgcaccagg ctgaaggtgt ttttcagatc cgcaccgaga
    22021 cacagcacag gcggaacatt tttaaagccc ggaggcaaag ccagcgcatc cggcacatac
    22081 ccccgcgaac ggcgcagcat ttcgccgctt tcgcgcacca ccgaatcatc catccgctgc
    22141 acgatgtcgc ggttatgtat caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc
    22201 tgttcgttgc tgatagccgg tggtttaccg ctcaggttgc cggaggtcat caccagcggg
    22261 cattgcagtt cctgtaacag caaatgctgg agcgggttcg caggcaacat taccccgact
    22321 tcgttaaggc caggggcgat atcatcacaa agctcaggta catatttttt atccaccagc
    22381 acaatcggcg cggcgggcgt ggtaagcaac tggcgcgcag cgtctggtaa accgtcagcc
    22441 actggcaaca tgaccgccag cggtttcgcc gggcgatgtt tgcgcgcccg aagtatcgcc
    22501 accgcgttac tgttacgtgc atcgcaggca agatgaaatc cgccaattcc tttgatggcg
    22561 acaatgttgc ccatttttaa ctgtgcgata gctgcctgta atgccgcctc ttgttctgca
    22621 tgttcaccat gacttaccca ttcaagatgg gggccacact ccgggcaggc caccggctgg
    22681 gcgtggaagc gacgatcgag cgggtcacag tactctttgt cacaagccgg acacagcgga
    22741 aacgccgcca tcacggtaaa cgggcggtcg taaggcatgg cgcgaataat ggtgaaacgc
    22801 gggccacagt gggtacagtt gataaacgga taacgccagc gtcgttcgcc tggggtattc
    22861 atttcggcaa ggcaagcagg gcaagtggcg gcatcgggaa caatttgcgt attcatgacg
    22921 ccacccgcgc tctggcggat ggtgaactca gtgggcagtt gtgaccagat aaacggctca
    22981 cgctcgacgc tatcaatacg cgccagtggc gggcagtgct gatgcaattg aacaagaaac
    23041 gtttccggat cttccagcag ccggacttct acgccatcgc cgtcattaca gacatcgccg
    23101 tgaagattta attgctgtgc cagttgccag acaaacggac gaaaaccgac gccctgcact
    23161 ttgccacgaa tacgcagttg gacaccgcaa gatgtgtttt ttgccattga gttattcccg
    23221 ccatcatgaa ttgcgtaacc cgcccagccg gacacgacag cgtcgcatcc ggcagtcaca
    23281 ggtcggcgat actgcccgct tcgtattcta cgaatatttc cgggaattcc tttgatacca
    23341 gaacagttct gtaagatttt tagaacatca gcgccgtgcg gcggcgtttt tctgcgctca
    23401 gttgttcaag tttattacga tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac
    23461 acgccgggcc gtcttcacga tggttgcaca ggtcgcattt attggcttcg gctttgtcag
    23521 cccgtacgtt aagccctgcg ccgctgttgc gaatcaccgg acgtaccacc acttccatcg
    23581 cgccatacgg gcaagccaca acgcaggttt tgcaaccaat gcagcgttcc tgcatcacat
    23641 gaacaaaccc tttatcacgg ctgatagcac cattcgggca gacgttagcg cacggtgcat
    23701 cttcacactg acggcagact gttgccgtag aaatgttcac acctttaatg acatggattc
    23761 gcggtaaaaa agtttccggg gtcagcgatg cacagtcctg attctcctga tgagaaacca
    23821 cgcacgccac ttcacaggta cggcaaccaa tacatttact cgcgtcagca atgatgaaac
    23881 ggttcatcaa attctccagc aatgacagtt aatgcgccga tacattcaca aatcatgcca
    23941 gtttttaatt tactgttatt taaggaaatt aaattctgta ttacaggaaa aacgatgtca
    24001 tcgacactag tgacgatgac atgtgatgac aatgtttatc gcgaaagagc aatgagtgag
    24061 tcgcggcgga tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc
    24121 ataaaaatca gccgtccaat aatttcctga atcatctcag tcaccggaat ttttacgctg
    24181 gagagcgccg gaacgatgta gggggcaatt gcgatatcat cgaatccgat aactgacacc
    24241 tgctctggca ccgctacgcc gcgctcgtgt aacgctttga tcgcacctat cgccatatcg
    24301 tcgttactgg caactaacgc gctaaattta gccccacgtt cgagcaacgt ttctacccct
    24361 tcggccccgc tggcgggcgt ccatttaccg ttagcgataa gtttttcatt gagagcaata
    24421 ctatgctgcg ccagcgcgtc tttatatccg gcaaggcgtt caatgctggt gggggaatcc
    24481 atcgaaccgg taaggaaagc aatctcctga tgtccgtcgt ttatcaactc tgccacggcg
    24541 ttaaagctgg tctgtttatg atcgcaccag acgctatggc tgctgttttt gcgcaggcgg
    24601 cgattaagca ccattatcgg ctgactgtgt gcgtcaatga tatcatcaat ctcatccacg
    24661 cttaaaaagc gcggataaat catgatggcg tcgcagcgca aatccagcag atactgaatc
    24721 gcctggcgct cttcttcggc gctatgttta ccatctgcca atagcaactg tcgccctttc
    24781 tcttccgcca ttcgcgcggc atgaaacagt aattcactaa aataaatgcc atggtaaagc
    24841 gtgttggtca ctaccagccc cagcgtctga gtactcttcg ccgacagatt gcgcgccagc
    24901 aagtttggac ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg
    24961 ctgacgtagc cattacctga aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc
    25021 ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat caacgcagtg
    25081 tactgcaccg tttgccgatt gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg
    25141 tttcacaaat ataaaaaatg aacaattaac tctcttgctt attgcatgtt aactattcat
    25201 gattttgtga aaccggtttc ctaattccgt ttcagcatcg gcatttttcc gtcacgtcga
    25261 ctgataacaa ctacatctac cctactgata acaggataaa atccgatggc caaaaattat
    25321 gcagcgctgg caagctcggt gataacggca ctgggcggcg ttgataacat ctcggcggtc
    25381 acgcactgta tgacgcgttt gcgctttgtt atcaaagatg accagcttat cgacagtccg
    25441 acgttgaaaa ccatctccgg cgtgctcggc gtggtacgta gtgacaacca gtgtcaggtg
    25501 attataggta atacggtttc gcaggccttt caggaagtcg tcagcctgct gccgggagat
    25561 atgcagcccg cacagcccgt gggtaaaccc aaacttacgc tacgtcgcat aggtgcgggg
    25621 atcctcgatg cgctgatcgg caccatgtca ccgctgatcc cggcgattat cggcggatcg
    25681 atggtcaaac tgctggcaat gatcctcgag atgagcggcg tgctgacaaa aggatcgccg
    25741 accttaacca ttctgaatgt gattggtgac ggtgctttct tcttcctgcc gctgatggtc
    25801 gctgcttctg ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc catcgcgggt
    25861 gtgctggtac atccgagttt tattgaactg atggcgaaag cggcccaggg tgaacatgtc
    25921 gaatttaccc tgatcccggt caccgcggtg aaatacacct acacggtgat cccggcgctg
    25981 gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg acagcattac accggcggtg
    26041 acgaaaaact tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg
    26101 ctgattggcc cgattggtat ctggatcggt agcgccattt cggcgctggt ttacaccatt
    26161 catggttatc tgggctggct ttcagtcgcc attatgggcg cgctgtggcc tctgctggta
    26221 atgacaggga tgcaccgcgt ctttacgcca accatcattc agaccattgc cgaaaccggc
    26281 aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg cggttcatca
    26341 ctggcggtgg catggaaaac gaaaaacccg gaactgcgcc agacggcgct cgctgcggcg
    26401 gcatcagcca ttatggcggg gatttccgaa ccggcgttat acggcgtggc gatccgcctg
    26461 aaacgtccgc ttatcgccag tcttatcagc ggttttattt gcggcgcggt tgctggtatg
    26521 gcggggcttg ccagccactc aatggcagcg ccggggctat ttaccagcgt gcagttcttc
    26581 gatccggcga atccaatgag catcgtctgg gtgttcgccg tcatggcgct ggcggtcgtg
    26641 ctgtcgttca tcctcacact gttgctcggc tttgaggata ttcctgttga ggaagcgact
    26701 gccgaggcgc gaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat
    26761 tgaggatgaa aatgtcagta tttccagaag gttttttatg gggcggcgcg cttgccgcca
    26821 accagtctga aggtgcgtac cgtgaaggtg gcaaagggct gaccaccgtc gatatgatcc
    26881 cacacggcga gcatcgaatg gcggtgaaac tgggactgga aaaacgtttt cagttgcgcg
    26941 atgacgagtt ttatcccagc catgaggcga cggattttta tcatcgttat aaagaagata
    27001 tcgccctgat ggcagagatg ggattcaagg ttttccgtac ctcaattgcc tggagccgcc
    27061 tcttcccgca gggcgatgaa ctgacgccaa accagcaggg cattgctttt taccgcgcgg
    27121 tatttgaaga gtgtaaaaag tacggtatcg aaccgctggt cacgttatgc cacttcgatg
    27181 tgccgatgca tctggtcacc gaatatggct cctggcgtaa ccgcaagctg gtagagcttt
    27241 tcagccgcta cgcccggacc tgctttgaag catttgatgg tctggtgaaa tactggctta
    27301 ccttcaatga aatcaacatt atgttgcata gcccgttctc cggcgcgggt ctggtgtttg
    27361 aagaagggga aaatcaggat cagatgaaat atcaggctgc gcatcaccag ctggttgcca
    27421 gtgcgctagc caccaaaatc gcccatgagg ttaacccgca aaatcaggtg ggttgtatgc
    27481 tggcgggcgg taacttctac ccttacagct gcaagccgga agatgtctgg gcggcgctgg
    27541 agaaagatcg ggaaaacctg ttttttatcg atgtgcaggc gcggggcgcg tatccggctt
    27601 actctgcccg cgtattccgc gaaaaagggg taaccatcga caaagcaccg agcgatgatg
    27661 agatcctgaa aaacaccgtc gattttgtct ctttcagcta ttacgcctcg cgctgcgcct
    27721 cggcggagat gaacgccaac aactgcagtg cggcgaacgt ggtgaaatcg ctgcgtaacc
    27781 cgtatctgca agtgagcgac tggggctggg gtattgatcc actcggtctg cgtatcacca
    27841 tgaatatgat gtacgaccgt tatcagaagc cactgtttct ggtggaaaac ggcctgggcg
    27901 caaaagatga atttgctgcc aatggcgaaa ttaacgacga ctatcgcatc agctatttac
    27961 gcgaacatat ccgcgcaatg ggcgaagcga ttgcagacgg cattccgctg atgggctaca
    28021 ccacatgggg ctgtattgat ttagtttccg cctctacggg tgaaatgagc aaacgctacg
    28081 gttttgtcta cgtagaccgt gacgacgcag gcaacggcac gctgacgcgc acgcgtaaga
    28141 aatcgttctg gtggtataaa aaagtgattg ccagtaatgg tgaagattta gagtaagtaa
    28201 ccgtgccgga tgcggcgtga acgccttatc cggcctacaa ggcccgataa gcgttgcgca
    28261 tcgggcaatc ttgggtttgg catcagtcaa aaatcaaaga aaccattccg gcttaacgcc
    28321 aaatcgggct gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaagat
    28381 ctggctgacc agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg
    28441 ttgatcaatc agcgtacgaa gtaacgccac gccaaccggc attgctgcaa cggctttgtc
    28501 gaactctgca aacttttcgt tattgttttc atattctgca atgcggctcg ccagaaagtc
    28561 gataagtgga ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg
    28621 ataatcatct tctgagtcac tgcctcccag aaaggggaca gcattgacca gttctcttgt
    28681 tgctttaaca gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgac
    28741 atctgtctac acataatccc cctcatagct actctgccaa aagtcagagc tggttcaccc
    28801 catcaagaac atcccccttt atcaagcaac gctgcgtcag caccagatct tccgattgcg
    28861 ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa gtaaaacgcg
    28921 tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat ttggcgtctt
    28981 ttacctgata cagactggtt atccagtaac cggaatcaaa ctgcatatcc agcaattgcg
    29041 tgtggccatt cctttgtaaa tccagcaaat catcaatgcc agtttcactt gcggtataaa
    29101 aactatcgga ctgaaaaaca cacgggcgac cttgctgatt aaaggtcatc agaatcaaaa
    29161 acgcgttagg tgccagacca tttcctgaaa taccccgcca gataaccaga tcctgaatgc
    29221 cgttacgatc aagatcggca cggtaaagtt gcgcattacc cgccatattt tgcagcaccc
    29281 ccacaggcac agacccggat ttaccggcgc gatctttacc gttcaattgc cattcactat
    29341 catcatttgg catcttttca agcgtcacgc cagcaacgcc aacttttcta aaatgccagt
    29401 tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt tgcgcgtatt
    29461 cgctgtcact catcggcaga tattcggtaa agctggtggc ctgtgcaggc agcactaagc
    29521 tcaataaaaa agcgcacatc cctgttctga tgttcaattc cctgaattcc ttactgaaaa
    29581 actactcttc ttccgccgct aactgcgcga agccgccatt tccttcccag ccttccagcc
    29641 gctgatacac cgtttccacc gcatctttaa tctgctgagt catcgggtag taaaagccga
    29701 cgatatccgg ctggatgcca aggaaaatca cttcgccaat atcttctttc aactggtcga
    29761 taagatagtt cagcggcatg ttatgggtag tcatcataaa catctcggcg atgtcagccg
    29821 ggtcgatgat gcggatctcg ccagggttta gccccatatc cgtggcgtcg acaataagca
    29881 gccgtgtcgg gcgcagttcg cggatagcga caatgtcgtt ttccggtgcg ctaccgccgt
    29941 caatcactac ccagttacct ttcggcgcgg cagcgcactt ttccgccagt agcgggcctg
    30001 cgccatcatc acccatcatg ctattgccaa cacagagtaa aacgtcagtc acgcagtctc
    30061 ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact taacagcatt
    30121 gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa tgcgttggcc
    30181 agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac gccttccatt
    30241 ttgcggcggg cttcgctgcc cgcctccaga gtggcaatcc atgccagata gtcatcccac
    30301 gggcaggtaa gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc aatcgccagg
    30361 ctgtaataga ccacctgctg cgcctcggcg ggcgtagcat cgttctcatc aatgaattta
    30421 cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct tcaacaacat
    30481 gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc agccagcgtg
    30541 caacctgttc ttcgccctgc cctaattgcg tcaggaaatc atcggcaatc tggcgaccgt
    30601 aacgataacc cgccaggcgg cgtgcttcgc gatccacttt cacgcgcagc ggctgcacca
    30661 tatcgccatg caggatctcc gccggttgtt catcctgctc acccggccca cgggcgtgga
    30721 ttttctgctc cagcaggccg agcgccatcg caaagccgta cagcgtggcg gcaggcgttg
    30781 gcgggcagcc agggatataa acatccactg gaacgatttt atccgtaccg ccccacacgc
    30841 agtagagatc gtggaagatc ccgccactgt taccgcaggc accgtaggag atacagattt
    30901 tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca cgggtgaccg
    30961 cgccggtaaa cagtaaaata tccgcatgac gcggtgacgg aacgacttta atgccgaagc
    31021 gttctgcatc gaacagcggc gaaagcgtgg cgaaaatttc gatttcgcaa ccgttacagc
    31081 cgccgcagtc cacgcgataa acgtaggcag aacgtttgat ttttttcagt aacgacgcct
    31141 tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg gcgtcacggg
    31201 ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt caatacggtc
    31261 ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc tttcgcggtg
    31321 gttttccgcg cggctgtcac cgttgtgctt aagcagcgca atggcgtagt cgatctcttt
    31381 ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc gggactgttg
    31441 caggaagtct tctttcttcc acaccgccag ttcgtactct tgcgacagct taatcgccgc
    31501 tgtcgggcag acttcttcgc agcgcccaca gaagatgcag cgcccaaggt caaactgcca
    31561 cgccagctca ccagtggcga ggtcggtttc aaccgttaag gcgtttgacg ggcaggcatt
    31621 gacgcaagcc gcacagccaa tgcactgctg cgggttctgc tctggcttac cacggaagtt
    31681 tttatcaacc gcaatcggct ccagcggata agacgaagtc gccgtgccgg ttttgatgac
    31741 ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt tacgctcaat
    31801 gctgtagcgc tcaagttctt tgtacggcac cactttgctc ttcttcttac gtacatcgac
    31861 cacggtcatg cggtcggtac aggagtagca agggtccagg ctaccgataa tcagcggcgc
    31921 atcggaaacg gtgttgccgc gcagcatata acgcagggtc ggccagttcg cgtaggtcgc
    31981 cgcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc agtggatatc
    32041 gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt aggtaaagcc
    32101 ttccaccatc agcgggccgc ccggcaggtt atccagaccg taatcgatca tattcagcgc
    32161 ggtatagact tcgttgatac gcactttcag acgggaaata acgtcgcagc cctgctcgct
    32221 gtggacttcc attggcagca ggccataacc aacaaacggg tgatcggcac gggtatcacg
    32281 ggcgtgaccg ctggcacgaa ccatcgggcc aacgttactg aagtcgcgag cgatttccgg
    32341 gtccagacga ccaatgccga cggtgcgttg ttccatgttc ggtgtgctca gcagcacatc
    32401 caccagctcc tgcacttcac gacgcatctg ttgtgccagc tggcgggtct ggatcatgtc
    32461 gtctttcagc agatcgcgac gaatcccgcc gatcaggttc aggccgtagg ttttacgtgc
    32521 accggtaagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga actgcataaa
    32581 gccggagtcg aagccggtaa agtgacaggc caggccgaga ttgagcagat gcgagtgcag
    32641 gcgttctacc tccagcagaa tggcgcggat catctgcgcg cgttctggca cctgaatacc
    32701 catcgcgttt tccaccgacg tggtataggc ggtgctgtga gcaaagccgc agatcccgca
    32761 cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg ccagtttttc
    32821 catgccgcga tggacgtaga acaggcggta gtcggcgtcg ataatgtttt caccatcgac
    32881 gaacagacgg aagtgacccg gttcgtcaga agtgacgtgc aggggaccaa tcggcacgac
    32941 gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat cggtagtcgg
    33001 tgccggacgc tgacgataat ccatgctgtc tttacgcaga ggataaagtt catccggcca
    33061 gtcatccggc agcaccagac gacgttcatc cggcagacca accggaatca aaccgtacat
    33121 atcgcgcact tcacgctcgc cccacaccgc cgccggaacg cgcggcgtca cggaaggata
    33181 ctccggtttg ttggcgtcaa cttcgacgcg aaccgttacc caacacttgg tgcccttctc
    33241 catcgacagc acgtaataaa cggcgtaatg accattcagt ttgcgttcgt cgttaccaaa
    33301 cagcaccgac agccaacccc cttgtttgta gtagagaaac tccaccactt ccggcaggta
    33361 gttcaccttc acggtgacgg ttagctgatc tttggtctgc caggcgtggt ccagcacgac
    33421 gccaggaaat gcctcattca gcgcggcgag ataatgttga cctaattttt cttcagacat
    33481 gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa tgcaaagcca
    33541 aacccggccc aggtaatgcg cggagtaata tcaagacgca gacgggccat gctgttttcg
    33601 aacagcgcga taaccaggac gccaaccacc agtttgacga tggcaatcac cagcgccagc
    33661 agcagcccac cgacggtgaa ggtttccatt tgtccccacg gaataaacac cccgacgaac
    33721 atctgcaaca ccaccagctg tttcaggctg atgccccatt tcatgacgcc aaagccgctg
    33781 ccgctatact cagagagcgg accttcctgc aactcctgct cggcttccgc caggtcgaac
    33841 ggcagtttgc ccatttcgat aaaggtggcg aacgcacagg cacaaagcgc cagcaccagc
    33901 gggatgctct ggctcagtgg ccagtgataa acggtgtcgg tgatgttgct gatgttggtg
    33961 gaaccggcaa cctgtgcggc gacccacaga ccaagcagca acatcggttc aaccaacacg
    34021 ccaagcatcg cttcacggct cgcgccgata gcggtaaacg ggctaccagt atccagacca
    34081 gaaatggcaa agaagaaacg cgcgatggcg aagagataca gtaaggtgat caaatcgcca
    34141 agttgcggca gcggagaacc gacggtcacc accggcagcg cagtggcgat agtcagcatg
    34201 acgcccacca tcacatacgg cgtcaggcgg aacacccagc cggaggcatc cgggccaacg
    34261 ctctggcggc ccagcagttt gataatgtcg cgatactcct gcaacacgcc cggcccgcga
    34321 cggttatgca gacgggcacg cgccacacgg gtaataccgg agagcagcgg cgcaacggca
    34381 aatagcacca gcgcctgaat taacggatat aaaacactca ttctcaggct cctcgtgaaa
    34441 caataatcac caccagcacc gccagttcaa ccagcgccat ccggcggaac agcaacgcac
    34501 tcccctcgca ctgccagccc ggcaccagcg acaccggatt caaccatttg cgtagtttca
    34561 gcaccggcgc aaacgcctgt ttcaccggca tcgcaaaacc gtgagcggta atcaccattg
    34621 atttttcatg atcgtagccg cacacccagg ccgcgccgcg ggaacgcgac ggcaaacgat
    34681 cgcctttgca aatcgccata atgatgaatg gcagcagcgg gcaggcaatc agcagcaacg
    34741 tgatcatcgg ttgagaaacg gtggtgttag caggctccag cggcagaggt acagcagcag
    34801 agagcatcgg cagtagccac ggcgcagcaa caccgccaat tacgcagcaa atcgccagtg
    34861 ccactacgct tacgctcatc aggagcggcg cacaggtggc gttttcggcc tctttggtac
    34921 gcggcgcgcc aaggaaagta acgccataaa ctttcgccat acacatcacc gccagcgcac
    34981 cggtaattgc cagccccacg gcgagcagcg gcccgagaag acgggcaaca aacgcgccac
    35041 tattgctcag tttgaagaag gattgataga taacccattc cccggcaaaa ccgttcagcg
    35101 gcggcagcgc agccattgcc atcagcccga ctaacatggc gatggagata accggcattt
    35161 tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac cagacgctcc
    35221 ccgcaccaag gaacagtacg ctcttgaaca ggctatggtt aagcagatgg tacagaccac
    35281 caaccagacc aagggcaatc agcgccggtt gttcgagcgc gatacccgtt acgccagcgc
    35341 ccagccccag caggataatg ccgatattct ccagggtgtg gtaagccagc agacgttgga
    35401 tattatgttc catcaacgca tacagaccgc cgacgaatgc ggtgatcata ccgagcacca
    35461 gcagcgcgat cccccaccac agcggtgcat taccgcccag tagtgacaga gttaaaatgc
    35521 ccagcaggcc aattttcatg actaccgtgg aaaacagcgc ggcagctggc gcagaggcgt
    35581 tggcatgtgc ctgtggcacc cagccgtgca gcggaataat cccggccagc aggccaaagc
    35641 caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata cgcatatcca
    35701 gcaggcgcag atccagcgtg ccgtaacgct gccacagcag ccagcaggca atcgccagca
    35761 gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg ttgctggtga
    35821 ggaacaccgc acacagggcc atgatttcgg ccattaccac gaacatgccg aggttgctgg
    35881 cgatgacggc acagacggcg gcagccatca acatattgat ctgcaagccg ttgcatttca
    35941 cctgcgcgtg gcgatgccag tcaatgttgt agaggctgac aaacagaccg cataggccga
    36001 gtgtaatcag ccaaatcgca ttaagcggag agatttgcac atcgtagctt accagcgaca
    36061 gcgcaccgct cacgccaacc gcgccagtca gcacagtgaa gcccgcggct gccgtataca
    36121 ggctacccac cgcgccgcca attccggcta tccagccact tagcgctttt tgaaaagaaa
    36181 agagaaatgc cagaacagcg gcggcgacaa accacgccac gccgctattg atcagggaaa
    36241 ttgcgctcat ttagcctctc cactttgagc ctgctgaaac aaggtgagat cgccaaagtc
    36301 cgtgttaaaa gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg tgttatccac
    36361 cagatgcagg gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt catcaaagct
    36421 acaaaggtca catttgacgg cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct
    36481 gacacgcgcc ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt ttgccggaat
    36541 atccagcgga cggctgccgg aaaattcaat tgcgccaaac gggcaggcga taccacacag
    36601 tttgcagctt acgcacagac tttcattcaa ctgcacagca ccatcaacac gagtgatggc
    36661 gttaaccggg cagaccaccg cgcagggtgc atcttcacag tgatgacaga gctgcggcgc
    36721 agattctttt tcatttagca tcactcgcag gcgcggcatt gattgcaggc cgtgctggcg
    36781 atgcgtctct gaacaggcag cctcacaagt gtggcagccg atacagagcg tggagtcagc
    36841 aattacaaaa cgattcacca ggcattcctc aggtgattgt catttttgac gaaaacatgc
    36901 cgatgaaatg tcattttcga cactcatcga cacgcccatc cccaaacagg cgtaacgcct
    36961 gcaaaacggg caaagcctca gctcatgcta ccgggctttg tccctttacc agttggctta
    37021 aattcaccgg cacattgttt tcaatggcgg tatataagct gtcgtaaacg ccagcgattt
    37081 tttcgagata gtgttccaga acttgttcgc gatcgcggat gttgaccttg ccatcaatca
    37141 tgccgtcaat gctcaactgc gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt
    37201 cgacgtaata ctcgatggtg tggctgttaa agccatccgc caaggtgacg taaatgcgaa
    37261 aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc gcagctcatg gcgtgatgca
    37321 ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt gcagtagatg tgccactggt
    37381 cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag
    37441 tcattgtcag gttacccgtt taacagagat gccagcttta agcattttct gtgccaactt
    37501 ttaattcatt gttattaaag cgatttctaa gctaaagatg aatttcgtcg ccgtgtcgac
    37561 gtgtcatttc gacatcatcg acattattca tcgcagggat aatcaacact ggcacaatta
    37621 ttgcttgtag ctggcaatag ttaatgggag gcgatatgca cgaaataacc ctctgccaac
    37681 gggcactgga attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg
    37741 tctggctcaa aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg
    37801 atctggtttg ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa gaacaagagg
    37861 ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct actgacccag cgcgtccgcc
    37921 gctgtccaca gtgtcatggt gacatgctac agattgtggc agacgacggt ttacagattc
    37981 ggcggataga aatagaccag gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca
    38041 acctctatat cgagggtgat gaacataacc ctcattccgc gtttcgtagc gcgccatttg
    38101 ccccggcggc acgcccgaag atgaaaatca ccggcattaa agcgcctgaa tttaccccca
    38161 gccagactga agaaggcgac ctgcattacg gtcatggcga agcgggcact cacgcgccgg
    38221 gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt gctggacaaa aataaccgtc
    38281 tggctgaacg caaccgcgcg cgctttgctg cccgcaagca actggtgctc aacctggttt
    38341 ccagccctgg ttccggtaaa accaccctgc tgacggaaac tttaatgcgc ctgaaagaca
    38401 gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc gcacgcattc
    38461 gcgctaccgg caccccagcg attcaggtga acaccggtaa aggctgccat cttgacgcac
    38521 agatgattgc cgacgccgcg ccgcgcctgc cactggacga taacggtatt ctgtttatcg
    38581 aaaacgtcgg taacctcgtt tgcccggcca gcttcgatct cggtgaaaaa cacaaagtgg
    38641 cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa atatccgcac atgtttgccg
    38701 ccgcctcgct gatgctgctc aacaaagttg acttgctgcc gtatctcaat ttcgacgttg
    38761 agaagtgcat cgcctgcgcc cgtgaagtca atccagaaat tgaaatcatc cttatttccg
    38821 ccaccagcgg cgaagggatg gaccagtggc tgaactggct ggagacacag cgatgtgcat
    38881 aggcgttccc ggccagatcc gcactattga cggcaaccag gcgaaagtcg acgtctgcgg
    38941 cattcagcgc gatgtcgatt taacgttagt cggcagctgc gatgaaaacg gtcagccacg
    39001 cgtgggccag tgggtactgg ttcacgttgg ctttgccatg agcgtaatta atgaagccga
    39061 agcacgcgac actctcgacg ccttacaaaa catgtttgac gttgagccag atgtcggcgc
    39121 gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg aatatcgcga gccggaacag
    39181 gtaatgcagt taattgagca tctgcgcgaa cgtgcttcac atctctctta caccgccgaa
    39241 cgccctctgc ggattatgga agtgtgtggc ggtcataccc acgccatttt taaattcggc
    39301 ctcgaccagt tgctaccgga aaacgttgag tttatccacg gtccgggttg ccctgtgtgc
    39361 gtactgccaa tgggtagaat cgacacctgc gtggagattg ccagccatcc ggaagtcatc
    39421 ttctgtacct ttggcgatgc catgcgcgta ccagggaaac aggggtcgct gttgcaggca
    39481 aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccaa tggatgcgtt gaaactggcg
    39541 caggagaatc caactcgtaa agtggtattc ttcggcttag gttttgaaac caccatgccg
    39601 accaccgcca tcactctgca acaggcgaaa gcccgcgatg tgcagaattt ttacttcttc
    39661 tgccagcaca ttacgcttat cccgacactg cgcagtttgc tggaacagcc agataacggt
    39721 atcgacgcgt tcctcgcgcc gggccacgtc agtatggtta tcggcactga tgcctataat
    39781 tttatcgcca gcgcttttca tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta
    39841 cttcaaggcg tggtgatgct ggtggagcag aaaatagcgg cccacagcaa ggtagagaat
    39901 cagtatcgtc gggtggtacc ggatgccggt aacctgctgg cgcaacaggc gattgccgat
    39961 gtgttctgtg tcaacggcga cagtgaatgg cgcggcttag gcgtgattga atcttctggc
    40021 gtgcacctga cgccggatta tcaacgattc gatgccgaag cacatttccg cccggcaccg
    40081 cagcaggtct gcgatgaccc gcgtgcgcgt tgtggcgaag tcttgacggg caaatgtaag
    40141 ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc aaaccgcgtt tggtgcgctg
    40201 atggtttcct ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa
    40261 gcgtgaataa tatccaactc gcccacggta gcggcggcca ggcgatgcag caattaatca
    40321 acagcctgtt tatggaagcc tttgccaacc cgtggctggc agagcaggaa gatcaggccc
    40381 gtcttgatct ggcgcagctg gtagcggaag gcgaccgtct ggcgttctca accgacagtt
    40441 acgtcattga cccgctgttc tttcctggcg gtaatatcgg caagctggcg atttgcggca
    40501 ccgccaatga cgttgcggtc agtggcgcaa ttccgcgcta tctctcctgt ggctttatcc
    40561 tcgaagaagg attgccgatg gagacactga aagccgtagt gaccagcatg gcagaaaccg
    40621 cccgcgcggc aggcattgcc atcgttactg gcgataccaa agtggtacag cgcggcgcgg
    40681 cagataaact gtttatcaac accgctggca tgggcgcaat tccggcgaat attcactggg
    40741 gcgcacagac gctaaccgca ggcgatgtat tgctggttag cggtacactc ggcgaccatg
    40801 gggcgactat ccttaacctg cgtgagcaac tagggctgga tggagaactg gtcagcgact
    40861 gcgcggtgct gacgccgctt attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc
    40921 gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt
    40981 gtggtattga actttcagaa gcggcactgc cggttaaacc tgctgtgcgc ggcgtttgcg
    41041 aactgctggg actggacgcc cttaactttg ccaacgaagg caaactggtg atcgccgttg
    41101 aacgcaacgc agcagagcaa gtgctggcgg cgttacattc ccatccattg ggaaaagatg
    41161 cggcgctgat tggcgaagta gtagaacgta aaggtgttcg tcttgccggt ctgtatggcg
    41221 tgaaacgaac cctcgatcta ccgcacgccg aaccgcttcc gcgtatatgc taataaaatt
    41281 ctaaatctcc tatagttagt caatgacctt ttgcaccgct ttgcggtgct ttcctggaag
    41341 aacaaaatgt catatacacc gatgagtgat ctcggacagc aagggttgtt cgacatcact
    41401 cggacactat tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc gcaactggta
    41461 aagcgttctg cgctcgccga caacgcggcg attgtgttgt ggcaagcgca gactcaacgt
    41521 gcgtcttatt acgcatcgcg tgaaaaagac acccccatta aatatgaaga cgaaactgtt
    41581 ctggcacacg gtccggtacg cagcattttg tcgcgccctg atacgctgca ttgcagttac
    41641 gaagaatttt gtgaaacctg gccgcagctg gccacaggtg ggctataccc caaatttggt
    41701 cactattgcc tgatgccact ggcggcggaa gggcatattt ttggtggctg tgaatttatt
    41761 cgttatgacg atcgcccctg gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag
    41821 atcgtttctg tcgtcaccga acaaatccag agccgcgtcg ttaacaatgt cgactatgag
    41881 ttgttatgcc gggaacgcga taacttccgc atcctggtcg ccatcactaa cgcggtgctt
    41941 tcccgcctgg atatggacga actggtcagc gaagtcgcca aagaaatcca ttactatttc
    42001 gacattgacg atatcagcat cgtcttacgc agccaccgta agaacaaact caacatctac
    42061 tccactcact atcttgataa acagcatccc gctcacgaac agagcgaagt cgatgaagcc
    42121 ggaaccctca ccgaacgcgt cttcaaaagt aaagagatgc tgttgattaa tctccacgag
    42181 cgggacgatt tagcccccta tgaacgcatg ttgttcaaca cctggggcaa tcagattcaa
    42241 accttgtgcc tgttaccgct gatgtctggc gacaccatgc tgggcgtact gaaactggcg
    42301 caatgcgaag agaaagtctt taccactacc aatctgaatt tactgcgcca gattgccgaa
    42361 cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg aaattcatcg tctgaaagaa
    42421 cggctggttg atgaaaacct cgccctgacc gagcagctca acaatgttga tagtgaattt
    42481 ggcgagatta ttggccgcag cgaagccatg tacagcgtac ttaaacaagt ggaaatggtg
    42541 gcgcaaagtg acagtaccgt tctgatcctc ggtgaaactg gtacaggtaa agagctgatt
    42601 gcccgcgcga tccataatct cagtgggcgt aataatcgcc gtatggtcaa aatgaactgc
    42661 gcggcgatgc ctgccggatt gctggaaagc gatctatttg gtcatgagcg tggggctttt
    42721 accggtgcca gcgctcagcg cattggtcgt tttgaactgg cggataaaag ctcactattc
    42781 ctcgatgaag tgggcgatat gccgctggag ttacagccga agttgctgcg tgtattacag
    42841 gaacaggagt ttgaacgtct cggcagcaac aaaatcattc agacggacgt acgtttaatt
    42901 gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc
    42961 tattaccgcc tgaacgtatt cccgatccac ctgccgccac tacgcgagcg tccggaagat
    43021 attccgctgc tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc
    43081 gacagcattc ctgccgagac gctgcgcatc ctgagcaaca tggaatggcc gggcaacgtg
    43141 cgtgagctgg aaaacgtcat tgagcgcgcg gtattgctaa cacgcggcaa cgtgctgcag
    43201 ctgtcattgc cagatattgc tttaccagaa cctgaaacgc cgcctgccgc aacggttgtc
    43261 gcccaggagg gcgaagatga atatcagttg attgttcgcg tgctgaaaga aacaaacggc
    43321 gtggttgccg ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac gactctgctg
    43381 tcacggatga agcgactggg aattgataaa tcggcattga tttaacagat gtggttatac
    43441 tcgatcaaca ttcaatgaaa ggaggcagta tggcagtttc agcaagaaat caattaaccg
    43501 gaacagttag cacagtagca atgggcgctg tgaatgatga agtagaactg acgctggcag
    43561 gtggtgcaaa gctggtggca attgtgactc acagcagcca gcaagcgctg ggtctggcga
    43621 aagggaaaga agcgattgcc ctgattaaag ccccgtgggt cacgctggca acggaagact
    43681 gtggactgaa attctctgct cgcaaccagt ttgctggcag cgtgtcgaca atcaccgaag
    43741 gcgcagtgaa cgccactgtc catatcaaaa cggatgcggg ttttgaaatt gtggcagtgg
    43801 taacaaatga aagtcaggat gaaatgaaac tcaccactgg ctcgcgcatt attgcattga
    43861 tcaaagcatc agccatcctg attgccacca aagcgtaagc gccgtatttg ccggagagta
    43921 tggctctccg gcagatttat ttctttggac gatatttttc tggcaactcc ggcaccggac
    43981 gcttgtcatc gatgagatga cgcacggtta agatcggatg acgccacagc attctcggcc
    44041 cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa cagtgcaccg
    44101 gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc cgcttttgcg
    44161 cgtaagcaaa caacgcctcg taatgctccg gctctgctga cgcctgcggg catttcgctt
    44221 gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc ttgccggaca
    44281 tgctgcctcc atactcttaa ggtgcattta tattacaact taattttaaa ggggactatg
    44341 tctctgaagg cggaggtttc ttcaaccgac gaatttggcg atcttgtttc atcgtcatca
    44401 cgcaaaagct gtaaagcagc atttttcgcg gaaccgacat caagaactca cctttcgcgt
    44461 cttcccctga aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata
    44521 aaaaccatca cactacattt aacatcaggg agccggactt aaccccatga gtacaataga
    44581 aaatttcgac gcccatacgc ccatgatgca gcagtatctc aagctgaaag cccagcatcc
    44641 cgagatcctg ctgttttacc ggatgggcga cttttatgaa ctgttttatg acgacgcaaa
    44701 acgggcgtcg caactgctgg atatttcact gaccaaacgc ggtgcttcag cgggagagcc
    44761 aatcccgatg gcggggattc cctaccatgc ggtggaaaac tacctcgcca aactggtgaa
    44821 tcagggcgaa tcggttgcta tttgtgaaca gattggcgat ccggcgacca gcaaagggcc
    44881 ggtcgagcgc aaagttgtcc gtatcgttac gccgggcacc atcagcgatg aagcactgtt
    44941 acaggagcgc caggacaatc tgctggcggc tatctggcag gacagtaaag ggttcggcta
    45001 cgcaacgctg gatatcagtt ccggtcgttt tcgcctgagc gaaccggcgg accgcgaaac
    45061 gatggcggca gaactgcaac gtacgaatcc agcggaatta ctgtatgcgg aagatttcgc
    45121 cgagatgtcg ctgattgaag gtcgccgcgg cctgcgtcgt cgcccgctgt gggagtttga
    45181 aatcgacacc gctcgccagc aattgaacct gcaatttggc acccgagatc tggtcggttt
    45241 tggtgtggag aacgcaccac gcggactttg tgctgccggt tgtctgttgc agtatgcgaa
    45301 agatacccaa cgcacgaccc tgccgcatat tcgttctatc actatggaac gtcagcagga
    45361 cagcatcatt atggatgccg cgacgcgtcg taacctggaa attactcaga acctggccgg
    45421 cggtgcggaa aatacgctgg cttcagtgct cgactgcact gtaacgccga tgggcagccg
    45481 tatgctgaaa cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca
    45541 gcaaactatt ggcgcattgc aggatttcac cgctgagcta cagccggtac tgcgtcaggt
    45601 cggcgacctg gaacgtattc tggcgcgtct ggcgttgcgt accgctcgcc cgcgcgatct
    45661 ggcccgtatg cgtcatgcct tccagcaact tccagaactg cgcgcgcaac tggaaggtgt
    45721 cgatagcgcc ccggtacaag cactgcgtga gaagatgggc gagtttgccg aactgcgcga
    45781 cctgctggag cgagcaatca tcgacacgcc gccggtgctg gtacgcgacg gtggtgttat
    45841 tgcgactggt tataacgaag aactggatga atggcgcgcg ctggctgacg gcgcgaccga
    45901 ttatctggag cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctgaaagt
    45961 tggctttaat gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc
    46021 accaatcaac tacatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga
    46081 gctaaaagag tacgaagata aagttctcac ctcaaaaggc aaagcactgg ctctggaaaa
    46141 acaactttat gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag
    46201 cgcgagcgcg ctggcggaac tcgacgtgct ggtgaacctg gcggagcggg cctataccct
    46261 gaactacacc tgcccgacct ttattgataa acctggcatt cgcattaccg aaggtcgcca
    46321 tccggtagtt gaacaagtac tgaatgagcc gtttatcgct aacccgctga acctgtcgcc
    46381 gcagcatcgc atgttgatca ttaccggtcc gaacatggga ggtaaaagta cctatatgcg
    46441 ccagaccgcg ttgattgcgc tgatggcgta tatcggcagc tacgtaccgg cgcaaaaagt
    46501 cgagattggc ccgattgacc gtatctttac ccgcgtaggt gccgcggatg atctggcttc
    46561 cgggcgttca acctttatgg tggagatgac cgaaactgcc aatattttac ataacgccac
    46621 cgaatatagt ctggtgttga tggatgagat tgggcgtgga acgtccactt acgatggtct
    46681 gtcgctggcg tgggcgtgtg cggaaaatct ggcaaataag atcaaagcgt tgacgctgtt
    46741 tgctacccac tatttcgagc tgacccagtt accggagaaa atggaaggcg tcgccaacgt
    46801 gcatctcgat gcactggagc acggcgacac cattgccttt atgcatagcg tgcaggatgg
    46861 cgcagcgagc aaaagctacg gcctggcggt tgcagcgctg gcaggcgtgc caaaagaggt
    46921 tattaagcgc gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgctgc
    46981 tacgcaagtg gatggtacac aaatgtcttt gctgtccgta ccggaagaaa cttcgcctgc
    47041 ggtcgaggca ctggaaaacc tcgacccgga ttcactgact ccgcgtcagg cgctggagtg
    47101 gatttatcgc ttgaagagtc tggtgtaata ataattcccg atagcctttt gctatcggga
    47161 atattaacga caactgacga atcaaataaa aataccctgt ataataggaa aacttatttt
    47221 acagggtaaa accatgccat ctacacgcta ccaaaaaatc aacgcccatc actatcgcca
    47281 tatatggacc gttggtgata ttcatggtga ctatcaatta ttacaatccc gcttacatca
    47341 actctctttt tgccctgaaa ccgacttact tatttcggta ggcgataaca tcgatcgtgg
    47401 gcccgagagt cttaacgtgc tgcgcctgtt aaaccagccc tggtttatct ccgtcaaagg
    47461 caaccacgaa gcgatggcgc ttgatgcatt cgaaactggc gatggaaata tgtggcttgc
    47521 cagcggtggt gactggtttt tcgatttaaa tgattcagag caacaagagg caatagatct
    47581 gttgctgaaa ttccatcacc ttccacatat tattgaaatc actaacgaca ccataaaata
    47641 tgtcatcgca catgcagatt atccggggaa ggagtatcag tttggcaaag aaatagcgga
    47701 gaatgaatta ctctggcctg ttgatcgtgt gcagaaatca cttaatagcg aattacaaaa
    47761 aataaacggt gccgacttct ttatatttgg tcatatgatg tttgataaca ttcagacgtt
    47821 cgctaaccag atttatatag ataccggatc gccgaaaagc gggcggctgt cattttataa
    47881 aataaagtag tctcatgctt cttctgtgaa gcatgagtaa cccggcgtta ttgcaggtca
    47941 ttattcattt ttcgctacca gcaaagagag atccagcttc gccagcgcgc ggctggcact
    48001 ctccggcaga ccatcgtcag taatgatctg atcaaactcg cttaatggta acgccagcca
    48061 tgtcgccacc tgtccatatt tcgtcgcatc acagaccaaa actcgctggc ggctggcact
    48121 ggcaatcgcc cgtttcaccg tgactttatc ttctgctggc gtagagatcc cccgcacact
    48181 ccatgacgat gcagaaataa aagcctgatc aatcatcagg ctacgcagca tggtcgcagc
    48241 ggcttcccca acacaggaac ggttttcccg acacactgcg ccgccagtgt gaataattgt
    48301 gcaattactg ttgtcgagca agtagtccgc aataacgaag tcgttagtaa ccactgtgag
    48361 tggctccatg tgagtcagat gctgtgcaat cgctaacgtg gtcgttcccg catccagata
    48421 gatacaacgt cccggctgaa caagacttgc cgccagcttg ccaatagccg ctttttgcgt
    48481 cattgccagc gcagttttta cctgatgaga aggttcatgc gccacgcgtc ccggagactg
    48541 gacgcccccg gacaccagca caacggctcc ctgctgctcc agtttttgta aatcccgacg
    48601 aatggtcata tgtgacacat tcattctgtc cgttagttca gcaatactaa caatgccttt
    48661 ttcagctacc atctcaagga tgatttggcg acgctctacg ggtatcaact tttgctcctt
    48721 cctttgtcct gctgacattc tacgctattt gcctgcgaaa cgtgcgcggc gcaactaacg
    48781 cttagttcac ataaaataac acgcaatgtt aacttttgtg aatcagctca ccatatcttt
    48841 atctttccgc gcttatattc acaacacaaa acaaaatatc acttaaatta acaaggagag
    48901 cagatgaaaa cgggatcgga gtttcatgtc ggtatcgttg gcttagggtc aatgggaatg
    48961 ggagcagcac tgtcatgtgt ccgcgcgggt ctttctacct ggggcgcaga cctgaacagc
    49021 aatgcctgcg ctacgttgaa agaggctggt gcttgcggag tttctgataa cgccgcgacg
    49081 tttgccgaaa aactggacgc tctgctggtg ctggtggtca atgcgaccca ggttaaacag
    49141 gtgctgtttg gtgaaaaagg cgttgcacaa catctgaaac ccggtacggc agtaatggtt
    49201 tcttccacta tcgccagtgc tgatgcgcaa gaaattgcta ccgctctggc tggattcggt
    49261 ctggaaatgc tggatgcgcc ggtttctggt ggtgcggtaa aagccgctaa cggtgaaatg
    49321 actgtcatgg cctccggtag cgatattgcc tttgaacgac tagcacccgt gctggaagcc
    49381 gttgccggaa aagtttatcg catcggagca gaaccgggac tgggttcgac cgtaaaaatt
    49441 atccaccagt tgttagcggg cgtacatatt gctgccggag ccgaagcgat ggcgcttgca
    49501 gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg tgaccaatgc cgccggaaat
    49561 tcatggatgt tcgaaaaccg gatgcgtcat gtggtggatg gcgattacac cccgcattca
    49621 gccgtcgata tttttgttaa ggatcttggt ctggttgccg ataccgccaa agccctgcac
    49681 ttcccgctgc cattggcctc aacagcattg aatatgttca ccagcgccag taacgcgggt
    49741 tacgggaaag aagacgatag cgcagttatc aagattttct ctggcatcac tctaccggga
    49801 gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt taccggcgcg acggatatcg
    49861 ccagttttct ggtggaaaac ggtctaccaa cggtacaaat taacggtgtt ccaacaggta
    49921 aaatgccgga agcaatcgac gcactggtga tcagcctgaa aacgcgctcc tgtccggtgg
    49981 ttgaagccac acagcaatcg ctggcggctc tgagctggtt gcaacagcaa ggttgcaaac
    50041 agatctattt caaatactgc tctactttcg acagtacggc gaaagggaat atcggcccag
    50101 ttaccgatgc attaatggat gctctcgaca caccgtttac ggtcttctct ccggccctgc
    50161 cagtcaacgg acgtacggtt tatcaggggt atttgttcgt aatgaatcaa ctgctggccg
    50221 aatccgggat gcgccatcac ccggtaaatc ccatgaccga cagctatctt ccccgtctgg
    50281 ttgaatcgca atccacaggg cgctgcggcg tcgtttcagc acatgttttc gaacaaggtg
    50341 tgaaagccgt tcgtcaggag ctggctcgct tacagcaaga gggctatcgc tacgcggtgc
    50401 ttgatgcgct gaccgaacac catctggaaa ttcaggggga agccttgcgc gatgccccac
    50461 tggtaacggg cggttctggt ctggcgattg gcctggcccg gcagtgggcg caagaaaacg
    50521 gtaaccaggc tcgcgaagca gggcgtccgc tcgctgggcg cggcgtagtg ctctccggtt
    50581 catgctctca aatgaccaac cgccaggtgg cacattaccg tcaaattgca ccagcccgtg
    50641 aggttgatgt ggcacgctgc ctctcaaccg aaattctggc cgcttatgca cacgagctgg
    50701 cagagtgggt tctgggccag gaaagtgtac ttgctccact ggtttttgcc accgccagca
    50761 ctgacgcatt ggcagcaatt caacagcaat acggtgcaca aaaagccagt caggcagtag
    50821 aaactctgtt ttctcaacta gcggcgcggt tagcagcgga aggcgtgaca cgctttattg
    50881 tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct gggcataaaa ggttttcata
    50941 ttggcccaac catttccccc ggcgtgccgt gggtaaacgc actggataag cctgtctcac
    51001 tcgcccttaa atctggcaac ttcggtgatg aagccttttt ttcacgagcc caaagagagt
    51061 ttttatcatg agcgatttcg caaaagtaga gcagtctttg cgagaggaga tgacgcggat
    51121 tgccagttca ttctttcagc gcggatatgc aaccggttcg gctggcaatc tgtcgctgct
    51181 tttacctgac gggaatttac tggcgacacc gacaggttca tgcctgggca atctcgatcc
    51241 gcagcggctt tccaaagtca ccgcggatgg cgaatggtta agtggtgaca aaccctcgaa
    51301 agaggtgctc tttcatctgg cgctgtatcg caacaatccg cgctgtaaag cggtagtgca
    51361 tttgcacagt acgtggtcga cggcgctttc ctgcctggaa gggctggaca gcaataacgt
    51421 tattcggccg ttcacaccat acgtggtgat gcggatgggt aatgtcccgc tggtgcccta
    51481 ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg gaactggcag cagacaatca
    51541 ggctttttta ctggcaaatc atggcccagt ggtttgcggt gaaagcctgc aagaagccgc
    51601 caacaatatg gaagagctgg aggaaacggc aaagctgatt tttattctcg gtgaccgccc
    51661 gatccgttat ctgaccgcag gtgaaattgc ggaattaagg agttaatgca atgcctcgtt
    51721 ttgcagctaa tttatccatg atgttcatcg aagtgccttt tattgaacgc ttcgccgcag
    51781 cgcgaaaagc cgggttcgat gctgtggagt ttctgtttcc ctatgactac tccacccagc
    51841 aaatccagca gcaactggtg caaaaccatc tgacactggc gctgttcaat accgctcccg
    51901 gagatattaa tgcgggagaa tggggattat ccgcccttcc cggacgtgag cacgaagcac
    51961 gcgcagacat cgacctggca ctggaatatg cattggcgct caattgtgaa caagtccatg
    52021 tgatggcagg cgtcgtgccc gctggcgaag atgctgaacg gtaccgggca gtatttattg
    52081 ataatctccg ctacgctgcc gaccgctttg cgccacacgg caagcgaata ttggttgaag
    52141 cactcagccc cggcgttaag cctcattatc tcttctccag ccagtatcag gcactggcta
    52201 ttgttgaaga ggttgcgcga gataacgtgt ttattcaact ggatactttc catgcgcaaa
    52261 aagtagatgg caacctgaca catttaatcc gcgactacgc cgggaaatat gcgcacgcac
    52321 aaattgccgg actacctgat cgacatgaac cggacgatgg agaaatcaac tatccgtggc
    52381 tgttccgcct gttcgatgaa gtgggatatc agggctggat cggttgtgaa tacaaacctc
    52441 gtggcctcac cgaagaaggg cttggctggt ttgacgcctg gcgctaattc gctcaatccc
    52501 taattacaac gtacccatac atccccccat ctacggatgg ggtaggttaa ctataacttt
    52561 cagacagggt ttcccatgtc cacaattaca ttgttatgca ttgcgttagc tggcgtaatc
    52621 atgctgttgc tactggtcat caaggcaaag gtacaaccat tcgttgctct gctcctcgtc
    52681 agcctgttag tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa agtgatgatc
    52741 gctgggatgg gcggtgtact tggttccgtc actattatta ttggtctggg cgctatgctg
    52801 gggcgtatga tcgaacactc tggtggtgca gagtcactgg ctaattattt cagtcgcaag
    52861 ttaggtgaca aacgaactat cgctgcgctg actctggcag cgttcttcct cggtattccc
    52921 gtcttctttg atgtcggctt tattattctt gcgccaatca tttacggttt tgccaaggtt
    52981 gccaaaatat caccactcaa atttggcctg cctgtcgctg ggatcatgct cactgttcac
    53041 gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg ggttactcca cgcagacatc
    53101 ggctggctaa ccatcatcgg tattgcgatt tctattcccg tagggattgt tggctatttt
    53161 gcagcgaaaa taatcaataa gcgccaatat gcgatgtcgg tcgaggtact ggaacagatg
    53221 caactggctc cggccagtga ggaaggcgcg acaaaattaa gcgataaaat aaatccaccg
    53281 ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg cgattatcat ggcgggtacg
    53341 gtttctgcaa cactgatgcc gccttcgcat cccctgcttg gtacgctaca gttgatcggc
    53401 tcaccaatgg tagctctaat gattgcgctg gtgctggcat tctggttatt ggctttacgt
    53461 cgcggctgga gcttacaaca caccagcgac attatgggct ctgcccttcc cactgcggcg
    53521 gtagtgattt tggttactgg tgctggaggg gtatttggta aagtgctggt ggaatcgggc
    53581 gtcggtaaag cccttgccaa catgttacaa atgattgacc tgcctctgtt accagccgca
    53641 tttattattt cactggcgct gcgtgcatcg caggggtcag caaccgtagc aatcctgaca
    53701 accggcgggt tactctcaga agcggtgatg ggattaaatc cgattcagtg cgtattggtg
    53761 acgctggcag cctgctttgg tgggcttggc gcgtcgcata ttaatgactc agggttctgg
    53821 attgtgacca aatatctggg gttgtcggta gcagacggtc tgaaaacctg gacagtgtta
    53881 acgaccattc tcggttttac cggattctta attacctggt gcgtatggct cgtcatctaa
    53941 taatccagat aagatatgat gtatcgttta agtgcctgat aagggggtaa tgtatgccaa
    54001 tattaacctc ggtaatatct cgctaccgag gttaattttc actttgcaat ataattacaa
    54061 tctcttgcaa gttgatttta tcttagtaat aagaaccgac caatcttatt gccatttcga
    54121 tattagcagg catagatgcg tcgtagccat acatatggcc atatttcgga cgtaatactt
    54181 cagttacttt cttaataatt gcttcgcgct gggctcgcgc aaaatcagca tgattatttg
    54241 ccgttttatt cataaattta ccaaaacttt cttcttttct gactttaatt gtcgtatccc
    54301 gaagatcatt aagaaactgg cgttgaaatt taaaatcatc gtgtgaacca atattaccat
    54361 gcccgccaat aaaatatttc cagtcaagca tttcaacctg acgcaaatta ctgtcatggt
    54421 aaaccaacgt atccgatacg gcgaagccca tcatcggcag ttggtcagga ttcaggagat
    54481 ccgggctgtg agctactttt tcctgtttta gcaaccatac agaatggtcg tcggtatgtc
    54541 ctgctttaac aaatcggtgt aattcaatgg tctgcttttc aaatttaaac tggtcatctt
    54601 ttttacttaa aacctgagtg gcgacgggta agcgactgtt cataaattgc attttttcag
    54661 cagtggcttt actggcaata atacgcagat tcacgccttc tttcttcgcc tcatcgttcc
    54721 aaaatggact atcgccaatg tgatccacat ggtaatgaga ataaaccagc gctgttacgg
    54781 gtaatggagt cacatcacga attgcctgta ataaatattt accgcgaccc tccggagcat
    54841 caaacagcaa aacccctttg tctccgacat aaaacgtagt ggaataaaaa tagcgttgaa
    54901 cataataagt tctctcgcca attttttgta aaatataagg ctgagacata tttctttcaa
    54961 aaaggttatt gagtttatcg cttggcgata ttattttttc cgctgaaggt tcaacataac
    55021 ttgcattaac tgtagacgaa tttgcagtcg aaatttccgt ttgagcaaaa acactaccag
    55081 atataagaga agaggctaga attgtatata ttttaatgcc ttgcatacgt agtcctttat
    55141 taatgtgttg ttgatttata gcatctctga acagactgtt attttagata ttactacgtt
    55201 aggagattca tttttcttcg aatattatgt ttatctcatt caaaaaatat gttcatcatt
    55261 tgatggttta aataa
//
LOCUS       DAZFRE010000022        54708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFRE010000022 DAZFRE010000000
VERSION     DAZFRE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.022"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..920
                     /gene="umuC"
                     /locus_tag="ACHKUI_001569"
     CDS             <1..920
                     /gene="umuC"
                     /locus_tag="ACHKUI_001569"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFO6597789.1"
                     /translation="FGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGV
                     VALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFS
                     VILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEK
                     LRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHVWR
                     EGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGR
                     GTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            1124..1750
                     /locus_tag="ACHKUI_001570"
     CDS             1124..1750
                     /locus_tag="ACHKUI_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFO6597790.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            1747..2049
                     /locus_tag="ACHKUI_001571"
     CDS             1747..2049
                     /locus_tag="ACHKUI_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597791.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(2576..3922)
                     /gene="yagA"
                     /locus_tag="ACHKUI_001572"
     CDS             complement(2576..3922)
                     /gene="yagA"
                     /locus_tag="ACHKUI_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO6597792.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            4276..4878
                     /locus_tag="ACHKUI_001573"
     CDS             4276..4878
                     /locus_tag="ACHKUI_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6597793.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            5116..5382
                     /locus_tag="ACHKUI_001574"
     CDS             5116..5382
                     /locus_tag="ACHKUI_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597794.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(5651..5854)
                     /locus_tag="ACHKUI_001575"
                     /pseudo
     CDS             complement(5651..5854)
                     /locus_tag="ACHKUI_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5960..6235)
                     /locus_tag="ACHKUI_001576"
     CDS             complement(5960..6235)
                     /locus_tag="ACHKUI_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6597795.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(6235..6519)
                     /locus_tag="ACHKUI_001577"
     CDS             complement(6235..6519)
                     /locus_tag="ACHKUI_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6597796.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(7427..8458)
                     /gene="repA"
                     /locus_tag="ACHKUI_001578"
     CDS             complement(7427..8458)
                     /gene="repA"
                     /locus_tag="ACHKUI_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6597797.1"
                     /translation="MAGLKNTSYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            9159..9800
                     /locus_tag="ACHKUI_001579"
     CDS             9159..9800
                     /locus_tag="ACHKUI_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6597798.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            9941..10603
                     /locus_tag="ACHKUI_001580"
     CDS             9941..10603
                     /locus_tag="ACHKUI_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO6597799.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            10870..11490
                     /locus_tag="ACHKUI_001581"
     CDS             10870..11490
                     /locus_tag="ACHKUI_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6597800.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            11517..13211
                     /locus_tag="ACHKUI_001582"
     CDS             11517..13211
                     /locus_tag="ACHKUI_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6597801.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            13292..13534
                     /locus_tag="ACHKUI_001583"
     CDS             13292..13534
                     /locus_tag="ACHKUI_001583"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO6597802.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            13650..13985
                     /locus_tag="ACHKUI_001584"
     CDS             13650..13985
                     /locus_tag="ACHKUI_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597803.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            14212..15282
                     /locus_tag="ACHKUI_001585"
     CDS             14212..15282
                     /locus_tag="ACHKUI_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO6597804.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            15286..15723
                     /gene="pilM"
                     /locus_tag="ACHKUI_001586"
     CDS             15286..15723
                     /gene="pilM"
                     /locus_tag="ACHKUI_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6597805.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            15755..17374
                     /locus_tag="ACHKUI_001587"
     CDS             15755..17374
                     /locus_tag="ACHKUI_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6597806.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            17395..18690
                     /gene="pilO2"
                     /locus_tag="ACHKUI_001588"
     CDS             17395..18690
                     /gene="pilO2"
                     /locus_tag="ACHKUI_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6597807.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTIPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            18680..19138
                     /gene="pilP"
                     /locus_tag="ACHKUI_001589"
     CDS             18680..19138
                     /gene="pilP"
                     /locus_tag="ACHKUI_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6597808.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            19242..20750
                     /locus_tag="ACHKUI_001590"
     CDS             19242..20750
                     /locus_tag="ACHKUI_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6597809.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            20752..21846
                     /locus_tag="ACHKUI_001591"
     CDS             20752..21846
                     /locus_tag="ACHKUI_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6597810.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            21908..22444
                     /locus_tag="ACHKUI_001592"
     CDS             21908..22444
                     /locus_tag="ACHKUI_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6597811.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            22489..22974
                     /locus_tag="ACHKUI_001593"
     CDS             22489..22974
                     /locus_tag="ACHKUI_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6597812.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            22990..23616
                     /locus_tag="ACHKUI_001594"
     CDS             22990..23616
                     /locus_tag="ACHKUI_001594"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO6597813.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            23634..24995
                     /gene="pilV"
                     /locus_tag="ACHKUI_001595"
     CDS             23634..24995
                     /gene="pilV"
                     /locus_tag="ACHKUI_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6597814.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            25109..25303
                     /locus_tag="ACHKUI_001596"
                     /pseudo
     CDS             25109..25303
                     /locus_tag="ACHKUI_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            25468..26289
                     /locus_tag="ACHKUI_001597"
     CDS             25468..26289
                     /locus_tag="ACHKUI_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO6597815.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            26391..27593
                     /locus_tag="ACHKUI_001598"
     CDS             26391..27593
                     /locus_tag="ACHKUI_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6597816.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            27697..28155
                     /locus_tag="ACHKUI_001599"
     CDS             27697..28155
                     /locus_tag="ACHKUI_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO6597817.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            28152..28988
                     /locus_tag="ACHKUI_001600"
     CDS             28152..28988
                     /locus_tag="ACHKUI_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO6597818.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            28972..30120
                     /gene="traJ"
                     /locus_tag="ACHKUI_001601"
     CDS             28972..30120
                     /gene="traJ"
                     /locus_tag="ACHKUI_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO6597819.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPVGEFPK"
     gene            30117..30407
                     /gene="icmT"
                     /locus_tag="ACHKUI_001602"
     CDS             30117..30407
                     /gene="icmT"
                     /locus_tag="ACHKUI_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFO6597820.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            30471..34532
                     /locus_tag="ACHKUI_001603"
     CDS             30471..34532
                     /locus_tag="ACHKUI_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO6597821.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            34549..34899
                     /locus_tag="ACHKUI_001604"
     CDS             34549..34899
                     /locus_tag="ACHKUI_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6597822.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            34911..35606
                     /locus_tag="ACHKUI_001605"
     CDS             34911..35606
                     /locus_tag="ACHKUI_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO6597823.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            35617..36591
                     /locus_tag="ACHKUI_001606"
     CDS             35617..36591
                     /locus_tag="ACHKUI_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO6597824.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSREIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            36595..37932
                     /gene="traO"
                     /locus_tag="ACHKUI_001607"
     CDS             36595..37932
                     /gene="traO"
                     /locus_tag="ACHKUI_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO6597825.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            37929..38642
                     /gene="traP"
                     /locus_tag="ACHKUI_001608"
     CDS             37929..38642
                     /gene="traP"
                     /locus_tag="ACHKUI_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO6597826.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            38639..39169
                     /gene="traQ"
                     /locus_tag="ACHKUI_001609"
     CDS             38639..39169
                     /gene="traQ"
                     /locus_tag="ACHKUI_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO6597827.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            39216..39614
                     /locus_tag="ACHKUI_001610"
     CDS             39216..39614
                     /locus_tag="ACHKUI_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO6597828.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            39671..39922
                     /locus_tag="ACHKUI_001611"
     CDS             39671..39922
                     /locus_tag="ACHKUI_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO6597829.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            39837..40655
                     /locus_tag="ACHKUI_001612"
     CDS             39837..40655
                     /locus_tag="ACHKUI_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO6597830.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            40950..43994
                     /locus_tag="ACHKUI_001613"
     CDS             40950..43994
                     /locus_tag="ACHKUI_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6597831.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            43994..44614
                     /locus_tag="ACHKUI_001614"
     CDS             43994..44614
                     /locus_tag="ACHKUI_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO6597832.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPKSLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            44572..45777
                     /gene="traW"
                     /locus_tag="ACHKUI_001615"
     CDS             44572..45777
                     /gene="traW"
                     /locus_tag="ACHKUI_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6597833.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            45774..46343
                     /gene="traX"
                     /locus_tag="ACHKUI_001616"
     CDS             45774..46343
                     /gene="traX"
                     /locus_tag="ACHKUI_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO6597834.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            46418..48577
                     /locus_tag="ACHKUI_001617"
     CDS             46418..48577
                     /locus_tag="ACHKUI_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO6597835.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTNTSSTEYRTAEAAEPKSIIMKVWSGENFADKIATSLSGTGTSSD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAKAF
                     LEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNMRRTPGVREEKLKNVNGDDKDGIKQ"
     gene            48665..49312
                     /gene="excA"
                     /locus_tag="ACHKUI_001618"
     CDS             48665..49312
                     /gene="excA"
                     /locus_tag="ACHKUI_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO6597836.1"
                     /translation="MSTTKSRYPSWWPYYYLVRNAYFIFGIPCLLFYAFFGTLIVHDS
                     ALSQTMDFIFAYGCWALIILPLLWLYLRTRAKKKKVQQVVQQIKESCHFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            49586..51172
                     /locus_tag="ACHKUI_001619"
     CDS             49586..51172
                     /locus_tag="ACHKUI_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFO6597837.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(51310..51462)
                     /locus_tag="ACHKUI_001620"
     CDS             complement(51310..51462)
                     /locus_tag="ACHKUI_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6597838.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            51792..52034
                     /locus_tag="ACHKUI_001621"
     CDS             51792..52034
                     /locus_tag="ACHKUI_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597839.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            52325..52693
                     /locus_tag="ACHKUI_001622"
     CDS             52325..52693
                     /locus_tag="ACHKUI_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597840.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            52697..52912
                     /locus_tag="ACHKUI_001623"
     CDS             52697..52912
                     /locus_tag="ACHKUI_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597841.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(53110..53733)
                     /locus_tag="ACHKUI_001624"
     CDS             complement(53110..53733)
                     /locus_tag="ACHKUI_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6597842.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            53893..54360
                     /locus_tag="ACHKUI_001625"
     CDS             53893..54360
                     /locus_tag="ACHKUI_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6597843.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            54510..54686
                     /locus_tag="ACHKUI_001626"
     CDS             54510..54686
                     /locus_tag="ACHKUI_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6597844.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
BASE COUNT        13875 a        14844 c        13803 g        12186 t
ORIGIN      
        1 tttttggtca tcagctcagg gaacaggtaa agagctggac aggactcacc atgggggtgg
       61 gcattgcgcc tacaaaaacg ctggctaaaa gtgcccagtg ggcaacaaag caatggccac
      121 agttttccgg tgtggtcgcg ctgacggcag aaaaccgtaa tcggatcttg aagctactgg
      181 ggttgcagcc agttggtgag gtctggggag taggacgcag actgacggaa aagctgaatg
      241 cgctgggtat taacacagca ctgcagctgg cgcaggctaa cacggcattc atccggaaaa
      301 acttcagcgt cattcttgag cgtacggtac gcgaactcaa cggcgagtcc tgcatatctc
      361 tggaagaagc accgccggca aaacagcaga ttgtctgtag tcgcagtttt ggtgaacgaa
      421 tcacagacaa agatgccatg caccaggctg ttgttcagta tgcagagcgg gccgcagaga
      481 aactacgtgg ggagcgtcag tattgccggc aggtgacgac atttgtacgg acatccccct
      541 ttgcagtaaa agaaccctgt tacagcaatg ccgctgtgga aaagcttcca ttgcccacac
      601 aggacagccg ggacattatt gccgcagcat gcagagcgtt aaaccatgtc tggcgtgaag
      661 ggtaccgcta tatgaaggca ggtgtcatgc tggctgattt cacaccatcg ggtatagcgc
      721 agccgggatt atttgatgaa atccagcccc gtaaaaacag tgaaaagtta atgaaaacac
      781 tcgatgaact gaaccagtcg ggaaaaggga aagtgtggtt tgcggggcga ggaaccgccc
      841 ctgaatggca aatgaaacgg gaaatgcttt ctcctgcgta taccaccaga tggacagatt
      901 tgccagtagc ccagttatga atatgtaatc cacccataat attgttatcc tgttatcctg
      961 ctatcttgtt atcttgttat cttgttatct tgttatctta attaatctat aaatcaaata
     1021 cttaacaact tgctaagttg ttaacataac aaaaataatt gcatggttcg cgttcagatg
     1081 gcaatattaa gttgttaact taacagctta atatgaggct ataatgataa tagtattagg
     1141 tagtcaaaag ggtggggtag gtaaaagtac actggcggta tctatagctg cgtatctgat
     1201 gtcactaggc aatagagtga ttattgtcga cgcagatgac cagaaatcag tattaacctg
     1261 gtacaacaat cgccctgaag atctccctca tattccggtt acaggcgcga caggtaacat
     1321 caaagccatg ttaaaggaac acgaaaaatc ctatgatttt gttattgcag actgtgctgg
     1381 ccgtgatagc gcagaaatgc gtagtggatt gatggccgca gatgtattca tttctccttt
     1441 gcgcccatct cagatggacc tggatgttgt gcctcataca tgctccgttt ttacagcagc
     1501 aaaagatttt aatgaggatg ttcggggata tctcgtttta aacatgacgc ccacaaatat
     1561 gtttgttaat gaagccaatg aggcagctga agtacttaag gattaccctg aaatgcatct
     1621 ggcaaactcc cgggtatgtg atcgtaaagc acatcgagat gcttgggccg aatcaatgac
     1681 aatttttgaa acgcaaaatg ataaagccca gcaagaaatc gaagcgttag tgaaagaggt
     1741 tattctgtga gaaatagagc aaacacttca cctcgtgacg aggcagcatt tattaacggt
     1801 ggcacggctg gccttgataa taccaaaaag tcatcagata aatcatcggt tgtaagcaaa
     1861 gcaaaaccag tgagtatttc tttttttgaa gaaaacctaa aagacattga tgcccttata
     1921 acggctgaga tggtttcagg taaaggcaga gtgaatcgtt ctgatgttgt cagggcggcg
     1981 gtagaagcac ttaaaacact atcaaaaaca gaggtatcac gattgatagc agaagtaaaa
     2041 caaagataac accttgagat aacaagataa caccttgtta tcttgttatc ttgttatctt
     2101 gttatcttgt tatcttgtta tcttgttatc ttcagtgtat tttaggatga cctatgttga
     2161 atagaaccta aaacaaaaca aatattaata ggggtccgct tggaaaaggg aaattttccc
     2221 acgctaagag aattgagttg aatgagtggt tacatccagg ccagctaact cactgaaccc
     2281 cgtacagggt cagacattcg gtatctcctt catcctctga ggaaaacaaa tgaattaact
     2341 gcttacgtat atggatacat atacagtatt aatttattaa ggagaaaatg gcatgtcaag
     2401 atttaatgat agtgattacc attaacatga agtaatgtta caccgttcag aaagcctgct
     2461 gacgctgtgg cagcatcaca gtctgagtat tctctggtgc aatacaaaaa ccggactttc
     2521 ccgtaacaac agacaatgtt acgggaaaag ttacagaact gagccacaaa atatctcagt
     2581 acctgagatg cataaaaaca ccgtacatat gtttctcaaa agggggatga tcgttatcca
     2641 gccatttcgg tggatcggta tgcagaacac caatctgctt catgccggta tgctgcctgg
     2701 attttctcgc gcagtaatca ctgtcttcat atcccatcgt ttcgatgacc actctggccg
     2761 tctttccgtc aggagccctt gctgtgacaa tgaaatccgg caggacatat ttcttttcac
     2821 cattaacata aacttctgta tcaaacaggg gcctttccag acttaattcc ggcgtacctg
     2881 gctttctttt aagccagctg gccacattca gaagagcagt gagcgtgttt cgttccagtt
     2941 tgctgtccac aggcaccgga catgtcagtt gataaagagc atgagcatac ccttcactgc
     3001 agatgattct gccatcggca ctccggcaaa aagcaagaat aacccagtaa gggggacgta
     3061 ttccgccctg cgcttcacca ttaatactga tcccccgcac aggtctgatg acctgaatat
     3121 taccgttcgc ccagtgaatt tccacagcat cacgacagat gtgctgcgcc ataaaaatct
     3181 gaaagaacat atgttttctt ctggcaggcc agtgtcgttc tgaattctcc agcgtttcca
     3241 ttaaccgctc ctgagacctt tcagacattc caggccggaa atggataatt tcagataagg
     3301 atcttccccg aatatactcc tgctgcagtg taacctgccg tatggcatca agacactcac
     3361 gataattagt ccggatacgg ggattagcca gggaatccag tttattcagc ccagcatcgt
     3421 caataagaga caggagtaaa cgggcaaggc gcggacgttt cttgcgaacg gaggagctct
     3481 caccattatg gtaagcagag cgggcctgca gtctgataac agaattactg tcatcaggtg
     3541 gtaaaaagtt tctgtaattt atacgggtgc taacgggatg ccggcttgct ccggaagatg
     3601 tttcctcttc atttccggat aactgacgat acatggggca ttcaggtgca tgctcatgat
     3661 taaaactgga cagataaaga gtacccgtat tgtttttaag agcagcaaaa ttcatggccg
     3721 gcgaatctcc cggaatacag tcacactgta accatgcatc atactcacgc atttcccgga
     3781 gaatatcatt ggccaggtta tgtagtgcca tggttgctgg ctttttcagt ccacagaccg
     3841 tctttactgc agtctcttcc tcactgctaa gctcccgaat cactttgccc cggggattct
     3901 tctttacaag tatcattggc ataatgaaag cactccttat gtacatgctt aaacagtaag
     3961 taaaatatac aaagaacacc ccatgaaaca aaggcagaga tgaactgtgc gccccgatat
     4021 cttcttctga tatcctttca aatgaattgt ttttatttat atcatgacaa cattaaagtg
     4081 agagaatata tcaaccatgt ttctgtcagg gttcagtaga atatcaattc gactgtcacg
     4141 ccataactgt tactacctcg agtataaata ctcagagaac ggacttatta gccatgaaaa
     4201 atcacctgat gggaaattta ctaatttatg aattgtgata ttattaaaga gtaaaaccag
     4261 ccagccgagg tatttatggg aaaaaatcag gaagaaagga aaactcccgt cattgtggtg
     4321 aaaaaacgtc gtactttttc gccaccgtcc ttatccgaaa aaacagatat tatcgcacct
     4381 gtatttactg aacagacggc agaatcagcc cccgccggaa taaattcatc tgccgtcgaa
     4441 acacatatcc ccgaagcgcc agccaggaag aagaaaaaga aaagacaccg ttttccacgt
     4501 ccctcccact ggacccgtga gtatactcac gagtgcgtgg aaaaaataaa ggccctgttt
     4561 cctcatcttc gtgctgaagg tggtggattt attcccctga aaattggcat aaacaatgat
     4621 atttcggcat ttctggcaga acacccggag acagaactga ccatggacga atggctctgt
     4681 gcagtgtcct gcatcacatc ccgccgcgta tatctgcagc ggacagctgt cgccggcgtc
     4741 ccacgttatg gcctggatgg tcaccccaaa gggcaggtct cagactctga agcacagagt
     4801 gccggtcgca gactggcgac attagaacaa aagtggctca ggatgcaggc acagcaggag
     4861 aatatttctg ggcaataaac actcctgata acaggtggac attacgtatc agaaaaaatg
     4921 ggaagagaat gttatgtgtg attcagaagc ggagaagatt ttattcatcc tgaataaaca
     4981 tgaacgggca tacagatgcc agtaccccgt ataacttgat aacatcagga attttattga
     5041 taaacagcag ttaacgaata gtgatactcc gaagaaaagc acggtcccaa atgaagataa
     5101 aaaaggcata agatgatgat cacaggaaat ggaataaaca ctgtaactgt caatggaaaa
     5161 gtgaaacaca tcacagaact ggatgatata acactttgtt tagagtggac aaaactacga
     5221 gaggaaaaca atcgtcttta tgaaattaat aaccaggcaa acagaggatg gcgtggtttc
     5281 atcctaagat tgataggggt taacctgcca gataaaagga ctgagtttac acaacgtctc
     5341 ttattgacgc gcaaaatttc gggttcggtc atgaaaaagt aacatattct atgctggaac
     5401 agaccaattt tgcattcgcc gtccaggagt ttgaggggaa agtagtcagt gtttacgctc
     5461 agtcggggaa agtgtgcaat gataaacaac acgaaaggaa tcgtagtaca tgggcaccgc
     5521 gattcaatat tcaaggaaaa cacaatacag caatgtactt gtactgtttc tattattctg
     5581 gcgatttcaa taaaggcact tgctcgcgct ctgtcatttt ctgaaactct tcatgctgca
     5641 tttcgatttt ccttctaaga tcataactcg catacctgtt ctttttatgt atatctatat
     5701 ctatttttgt gacgagttca aaccacaatg ttcgcactcc ccaggcagcg ttatgaaaca
     5761 tttccgcaac ggctatagag tctgtattaa ctgcttcggt catgtagtcg ctaatacgtt
     5821 cagcggtaat tttcgttatt tcatcgtaat tcattttatc tcctacaaga ttatttaatg
     5881 gaatgaaaaa tatctggcct gataataatg ttccagaatt aatatctgca ggctatgtta
     5941 agttgtcccc acacacaatc tacctgtttt ctcgtgtgtg ccacaggcgc aatacataaa
     6001 tagtctggcc attaagttcg taacggattt cgtactcgcc agcaaaaatc cgtctgacct
     6061 ccctgggttc aaactgaaac aactgttctc ccatacgtgg atgagttaac agaatgaccg
     6121 gtgcctgtgt cagggattgc accgttctgg cggccgcagg tttactggcc agcaccagaa
     6181 aatcatataa ccgcgccaaa tcagaaagcg ccttactggt ccacttcagt tccatcagcg
     6241 tggcaccggt aacggattgt cagtactgag gctgtccgcc caggcctgta cagcctgatg
     6301 gtcgatgacc tgtccggatg tcacatcgtc cagggcttcc agcgtcaggc gattacgctc
     6361 ctcctcctgg gcaagccatg cagaaagcgc ctgtttgata acccagcccc gggagcgttc
     6421 cagcctggcg gccatctggt cgactttatc agccataggt aatggcacat gcgcggtaat
     6481 gacttttgtt gcctgtgcgg tattgttttt catttgcata tccgattagt tgttaatcat
     6541 atttaatcat agtgattact ttcagacgaa acaaggcaaa taatgcgcgt cagcgcatgt
     6601 aaggcggcat acggtggtta catgtagcac aggtgtgaaa ccgccgaagc ccggcgtaag
     6661 ccggtactga ttgatagatt tcaccttacc catccccagc cctgctaagc catacccgtt
     6721 ttcagccatg acagagcttc tgtgcgcggt cggagtggtc ccgacgaggg tttacccgaa
     6781 gtcggggcgt atctccgcgt tagcgggccg tgagggccgc ttacgagcgt gtactcaata
     6841 cttccagcga gaagactgac agcgatgacg gagtatagtt acaacattca taattaaaag
     6901 cgatgttgtt cccgcccttc gggcgggggc ggcctcgctt ttcagttatg aggggtaggc
     6961 acggtgctgc gtttgtgtgg tgtttttatt tctgatgtct ttttgttgtg gtttcagaac
     7021 ggtgaggttc ggggcccgcg cgtcagcgtg ggaggtgctt ttagattctg agcggcgcgg
     7081 ccttggccgc tgcaagcgaa ggcctctttg tatttgttca cagtggtttc agagataaag
     7141 actttccctg cggcgaagca acgcagggga ttaaacgatg gggttaagaa tatattgcag
     7201 gtttgctgtt tcaggtttgc agcttcttaa agttctttgt ttgcggattt atgtggataa
     7261 ctgaaacaac gtggccacgt tctgatgact gatgcgtggt tttatgggca gatatgcccc
     7321 tctgagggga tttaaggtga ctactaagga ttttgggctg agcggaatct gcgccgaaag
     7381 ccccggaggg gctggcgatg attcagtgct gagggaatga tgctgtctag tgcggtggcg
     7441 gggcagccag cgtcagttca agctgacgca actccctgat ggccagtcgc ttcagatggt
     7501 catcggaaca gaaataggct tcatccggcg gcagacgctt ccggagatac tcagccatct
     7561 cataaatctg ctggtcaaca ggtaatttct tcaggcggtt ggcacgcttg ctggctgcgg
     7621 ctttttctcg gcgcttcttc agggcatcct ggctgcgctg cagataccag cgttttctgg
     7681 ccgcatgaac ggagatatct tcatcctcac gcagaacacc ttcccgaatg agctgctggc
     7741 ggatttcact ttcacgcagt cgtttctgct gctgttcgtg aagttttacc atgtcgacgc
     7801 ccagcatctg ccagcctgcc ggtgttatcc agacgtaacg tggcagacgc tgacgatgtt
     7861 cacggtccca cattgtttcc tctgacacac ccagcacacc aaagcgcacc tgttcggcca
     7921 gcagacggga aagacgggag accgtcactt ccagttccgg aataacatgt ccctcgctgt
     7981 ctttcgggct gatttcttca gccagccgag tgacgctcat ccccacggta tgtgtgccgg
     8041 catcactgaa gctgaccaga accggccaga tggcatcgag gagacgttgt ttttccggcc
     8101 ggaaagcgta tttgcgaccg acgaacgtcc gtttctgtac ataaaaagga tgccgcgaca
     8161 tgcgcatgcg caggctctgc tcgccggtca ccgggtcctt tttcaccaga cggttatagg
     8221 cgtgcccgag ctgccctttc agcgctttat agcgctcagg acgataccag gacggatttt
     8281 cgcatttggg tttggtggag tgctcaccgc gacggcgctt cgtgcgcttc ctttccggtt
     8341 caaccaggaa agtggtcgcc cttcccgctt catagtcttc ccagaaacgg atctgctctt
     8401 cgggtgctaa ctgactccag tgtactgcgt tatatgaagt attcttcaga cctgccacaa
     8461 gctgtcgctt atggcggagc gattgtgccg tgtatttata cactgcacag ggttgaatct
     8521 ctggtacggt ttcatatata cttatcccgt attctgtgag gcccccatta tttttctgcg
     8581 ttccgccaag ttcgaggaaa aatagtgggg gttttccttt atggtcttct gaaacttttt
     8641 tcagccttcc ggcaccggca aactgccgtc gtttctgcca tacgtcctga atgccttatg
     8701 gcgcattctg ccgttttctg gctttccgga ccgcgaggat cccaaaagcc actgaataac
     8761 gatccgcaat ataccccttt tccaccgctt tgaaaagccg acacgatttc tcagagttac
     8821 agttgccctc tttaaccccc taaaaagcgt cgtttttttg aactctgtca aaaaaatgaa
     8881 caatttcaca aacgatcttt tatgctgctt ttttcttctt ttccagtaga agagatcgcc
     8941 gaattttttt aaacgatcta atatcccgca cacaacatca aatacagaat aatattctgt
     9001 cttatcaaat ataacgatct attcaactgc ttatcttcgc tcttgccaca gatacatcct
     9061 tatgtgatat attttccagc cattaatcct gtttgcagga gttgtcggtt ttcgcccgcc
     9121 tcgaaaccgt ccacaaggct tacaagagtg cgtaaatcat gtgcctgtca gaactgcagg
     9181 ccaggctgaa cgtgggtcag ccgctgtttc cgacacatgc tttatgtatt cgccgttcac
     9241 aacaacatcg aagcccccct gcggggatcg attttcgcgg agcgttccgg attttgaata
     9301 tcagtgaatt acatcaacgc aactggtatc tggcacagta catccctgcc ggcaaaaacc
     9361 gcgaacatct cttttcctgg ctttccgaac aacacgtcct tccctggaca cccctgatac
     9421 tgaaaaaggt ccgccgcacg gataaagtct gtggttaccg tcgacatata cacgccgtgt
     9481 ttcccggtta ctttttcctg aaagcagacc ccgaaagcca cagcttcact caccttcgcc
     9541 gtcacagtgc ctttctggac ttcgtgaaaa tggccggtga aatcaaaacc gtcagggagg
     9601 atatcgttca gtctttgatg aaagtgtacc cggatcctgc gctcaatccg gcagccaggg
     9661 aggagctgga tgctgcctcc accctctggc tgacaaaagc gcgttaccag tacctcctcc
     9721 ggcttgatgc acagcctctg ccggagtcga ggatcgccct gctcctgcac ctggtatctg
     9781 atgacggagc gttgacatga aaattgctgt cttaatttga cgacttttcc cggcctttat
     9841 ggccgggatt tttttccctt ctgtttccac aatgcccggc accggtgaca cctgagggtg
     9901 tacggagagc ggctgtcggg cattttttca ggacgccttt atgaccattc tatcccagga
     9961 aaaccctgtc attctgttac tggagaatat gctcgaagca ctctccaccg ccccggacaa
    10021 cataaacaac gaacggcgtc gccggcgtta cctgctgaac tggcttgaca ctgcccgtca
    10081 gatgcgcgag ttcagaggaa tggctgaaga gttcactacc ctgcgaaaac tgctggccac
    10141 caattcagac ataccggtag caaacaccct gaaatcactg ctggaaagtg gcaataacgc
    10201 agcattatac gacctgtacc gcttccgctc ggctcttgcc acgtgcagga gtctgggatg
    10261 gcagattggc acgtgcgcat ggaatgacca gttactgagt gagacacttt cccgcgcaca
    10321 gaccgggaaa cgtcatatcc tgcagctgaa cagcaccggg gacacctttt ccaccacggg
    10381 cagaatgatc agaccggtgg cattttcatt aatccatccg gccaccatca acacgtctga
    10441 ggttgaagag atattccggg atgaaggttt tatgctcgca cacgggcgcg aatgcagtct
    10501 gaacggcagc ggcagaaata tgctgtcacg cattcttcat gtgggccatt caggtttagc
    10561 ggaagaggaa tggggcatgg acaacagtct gcttttacac tgaaataccg ggacagccac
    10621 aacggcagcc actctccgct gacttttttt cgcaaatcgc ggtttgctga tgtggatgtc
    10681 ccatttttga ttatccgtcc tctccggttg tcataaagcg cattccggcg tgaggcttct
    10741 gttgttatca tcccctcctg cagacatccc cttgtcttct gatgactgac gatatgcttc
    10801 ttctgacaat taaagaagcg gaagcttcac gccataatgt tgtttgactg acctgccaca
    10861 ggaggaaaaa tgacactgac gttacttacc gcgtataacg caccctatct ggcagccctg
    10921 acttttgttc tgcttactgg tatcgctgaa cttatcgccc tgctctgcgg acactccctc
    10981 tcttcagcta tggatacacc tgatttaccg gagggtctga caggagaggc gctggactgg
    11041 ctgaacatcg gacggatccc cctgctgatc gtcctctgta tgctggcagg tttttttggt
    11101 atatccggca ttctcctgca gggtcttatt attcatctgc tgcaggctcc tgctccgaac
    11161 attctccttg caccactttg cctgctgctg acatgtcctc tcgtacaccg caccggcaga
    11221 ctgattgccc cctggttacc ccgtgatgaa acaacggcgc tcaccgagga ggcattcatc
    11281 ggctgtatgg ccaccatcac tggttatcac gcagtggcgg gttccccctg tgaaggtcgc
    11341 ttcacagacc gcttcgggca ggttcattac ctgttgctgg agccggaggc cgggaaagaa
    11401 ttcagacgag gagacaaagt gattattgtc tgccgtcttt ccggttcccg ctggctgggc
    11461 gaactgaatc cctggccgga tgttctttaa tcccgacata tacagaggtt ctcactatgt
    11521 ttaatcttcc gggatggttg tttaccgtta tagcgctggt cctcaccctg ttcgttatcg
    11581 ggctgatatt tgcccgcctg taccgccggg catcggcaga gcaggccttt gtccgcacag
    11641 gtctgggagg ccaaaaagtg gtaatgagtg gcggtgcaat tgttatgccc atttttcacg
    11701 aaacaattcc cgtcaacatg aacaccctga agctggaagt cagtcgtgca gcggctgaaa
    11761 gtctcataac ccgcgaccgc atgcgtgttg atgttgccgt tgcctttttt cttcgggtaa
    11821 aaccatcagc cgaaggaata tccactgcag cccagacgct gggacaacgc acgctcacac
    11881 ctgaagatct gcgtagtctg gtcgaggata aatttgttga tgccctacgg gccacggcgg
    11941 ccagaatgag catgcaggat ttacaggatg cccgcgagaa ctttgttcag ggtgtgcaga
    12001 acaccgttgc ggaagactta tccaaaaacg gtctggagct tgaaagtgtg tcgctgacaa
    12061 gtttcaacca gactgcacgg gtacatttta atcctgacaa tgcctttgat gctgaaggcc
    12121 tgacccttct gacccaggaa acagaacgtc gtcgtcgcga acgcaacgaa gttgagcagg
    12181 atgttgaagt ggccatcagg gaaaaaaacc gggatgcact ctcccgacgt ctggagattg
    12241 aacagcagga agccttcatg actctggagc aacaacaacg ggtaaaaacc cgtacggcag
    12301 agcagagtgc cagtattgct gctattgaag ctgaacgacg tcgtgaagca gaatccgcgc
    12361 gaattctggc tgaacgtaag atagaggaag ccgaaatcga acgacagcag atagtgcgta
    12421 cccgccaagt cgaagccgaa cgggaagtgg ccatacggga aattgagcaa cagcaggcaa
    12481 ccgaaatcgc cagccaggcc agagccattg ccgtggcggc aaaatcggaa gagcagtcac
    12541 aggcagaagc ccgggcaagt aaagcactgg ctgaggccgt acaggcccag caggatgtgg
    12601 aaaccacccg ccagactgcg gaggccgacc gtaccaaaca ggttgccctg attgctgctg
    12661 ctcaggaagc tgaaacgaaa gcggttgaac tgacggttcg cgcacaggct gaaaaagaag
    12721 cagccgaact ccaggcagca gcaattattg aactggcgga ggccacccgt cagaaaggtc
    12781 tggcagaagc agaagcacag cgcgcactga acgatgccat taacgtgctt tcggatgagc
    12841 agaccagcct ccgcttccgt cttgccctgc tggatgcatt acctgccgtc atagagaagt
    12901 ccacagaacc aatgaaatcc attgacggta ttaaaatcat tcaggtggat ggtctgaatg
    12961 gcggtgttac ccgcggtaaa acagcaacaa ccgacataag tggcggaaat ctcgctgaac
    13021 aggcagtaac cgccgctctg gcttaccgta cacaggctcc gctcatcgac tccctgctga
    13081 aagaggtggg tttatccggt gaaggactta cagccctgac acgtccactg gtcatgcctg
    13141 ccccggaaaa ggttccggat gtcagcgaac cggtaaccgc tccggataac tcagaaccac
    13201 aacaggttta atccccgtgc cggaattttt ccggcatctt tttcacaaag aatgccctca
    13261 cgggatattc tggtttaaaa gaggattgaa aatgtccgca gaaaaattca gagttgtcat
    13321 atacaccaat acatgtgaaa agaaagtcag ttatattcct gcaacagaag atataaacat
    13381 cgctataatg ccatttcaga cggcagacaa tggcttaatt attgtgtgtc agaactcccg
    13441 tataatacga cgcaaagagc gtattcccgg aagcagctgt gaatggaaag atgtgacatt
    13501 agcaccaaaa tccagaaacc gcgttcagtt atagcctgac agcctgcttt tcatccccca
    13561 taaaaggggg taaaaattaa atatttcaca accgcgatat ttttcaggaa tgcagattat
    13621 tttctgttat gaccaattga gaaacaggca tgagaacaat cacaacacgt gaacaattat
    13681 taattaatgg gagagtccac gaaagaatcg ccacacatat agttacagga gctcatgggt
    13741 atgaaacgct ttgcacaaat ggctataacg tcagatacaa caaggcgcag caactggttg
    13801 aaagctgcga aaaaatagct gaaggtaaac tacctgttac ctgtcctgtc tgtttcacca
    13861 tatggcagga tgtacacaga ttcacccacg ttgactttga cacacaatcg gggaaaagtg
    13921 attttattga tacctgccat tcggagatca taaccggaca tgtttcaata acaggcaaga
    13981 aataaaataa tgttccagtt ctgaaaaaca atatcacttg ttttctggaa actatcattt
    14041 gcagcaggac gtttttcttt ttatatttcc ggaaccggaa tgttttagtt ttcataatca
    14101 ccccgaacat gaatgttcat catcaatatc ctgatgaaat tatatttatc tgaagacctg
    14161 ttaccagcca cctgtcttat accgtaaccc gatacatacc ggatttattc tatgaaaaag
    14221 aaatcactta cactccttct gccgtcatta ctcagcggct gtacggcatt taatgccacg
    14281 cctgttcctg acaccacgcc tccggctctg gtgttcgtcg acggacaaat tcgcgaaagc
    14341 gctgccatta tcgcccatac gcaaaaacgt ctgcttccgc caccggtgac ccgccctgtg
    14401 acgccgcccc gcccgacaac aacagcgacg cccacaaccg cagtcagcag ccagagtacc
    14461 gcactgcctc cccgtccgct ccttcaggcc acacagctgc ccgcactggt tatgaccggt
    14521 aaacccggtc agtcatccgt cgtttattta cgtgaggcac atcagcgctc cgtcagtgac
    14581 tggatacgcc agttgctacc acccggctgg cagttccggc aacagaacgc tgccataccg
    14641 gcactgaaca aacgcctccc cgtctggtcc gttaatgacc agtggcaccg ggcactgaat
    14701 gcgctgctgg ccggccagca gctttacgga catatggact ggaacaggaa aatactgact
    14761 gtcacgacct ctgcaacacc gccggtcgac gtcacggcac cgcagggttc acagaaagct
    14821 gctgatacac cgcgtaatcc gttcaggggc agcaccgcaa cgccggcagg cccgacacca
    14881 gtgacacctg ccgtcagcgt ttcccgggct cagaccacca ccgggacatc cggaactcca
    14941 gagagcagaa caaccacgcc agccccggtg gccacgaaac cggtagtgcc accacagcag
    15001 aaggtcatac cgccacggca gtggaaaatg gaaaagggga ccaccctgaa agacggactg
    15061 gcccgctgga catcatccga aaaatgcccg cacggcacat ggacactgcg ctgggaaaca
    15121 cctgtcaact acagcatcga tgcccctctg gttttcaccg gcagtttccg ccaggcgctg
    15181 gagcagctct ttgcactcta taccccggct gaaaaaccgc tgtatgcccg gaccagcact
    15241 gcacaatgcc tgctccgtgt gacagaccgc ccgcaggaat aacctatggg atgggttacg
    15301 ggtttcatta tggtcattat ggccattggt aatttctggt tccacagtca cctcagccgt
    15361 actgtgcacc atcagcagac cgctgaaatc acacagcagg ctgcagactt tattcgttat
    15421 atgaatgcca tcaacgatta cctgtatcag caccctgaac gacgtgctgc cggtggtcag
    15481 ctcacgtcag cacagctcgg tctgcccgcc acgaaaaacg tcagccacct catcagtcag
    15541 cagcgcgttt ttgtctgggc aaaagaaaaa cccggcctta tgggtgccct cctggaacaa
    15601 agtggtgatt cggccctgct tgccagagtg gaaaacggtc gcctcctgga cacccgtggg
    15661 cggcgtattt ccatcacgct ccctgctgtg atccccgatc aggtcatcat ctggatgaat
    15721 taggccggac tctgaataac tcacggattt atctatgaac aaaaaacttc ttgctattat
    15781 gattgcagcc actctgctgc agggctgtac cagtttccga caggcagaga acaccagaaa
    15841 agacgccgtt gctgaacaac aacgggcccg ggaaatgatc gctgagctgc gaaaccggac
    15901 accggttgta aaggaatccc cacgtcagtg gatcaatccc cgtccggtca cggataaaaa
    15961 ccccggtaat gcggtgcccg gctgtccgat tatcattaac acccggcaga gcatcaccct
    16021 tcagcaggtg gcccagcgga tctcgcaaaa ctgtcatatt cctgtaagga tcacccctga
    16081 tgtacaggcg tatctgagcg tgaccacctc cggcagtacg cagcaaatcc agggcacagt
    16141 accaccgccg gataccggcg gtatggttcc actggccgcc ataggcagtt ctgacacgac
    16201 acttccccgc ctgcagggaa atacggcgtc acttcctgaa ctgaaggctt caggactgac
    16261 atcactgctg aatgcggtcg cttcccgtct cgggatttcc tggcgttatg atggttcaca
    16321 tatcacgttc tattatcttg atacccggac cttcccggtg acctatatgg acagccaggt
    16381 tgcctataat gccaccgtgg tcagcggtac catgtcatca aatggctcat caggtgggac
    16441 agcatcggat gccatttccg gtgatgccag taatacccag accaccaccg tggatatgaa
    16501 atctgcgctg tatcaggatg tgaaaaatgc tgtctcatca atgctgacgc cgggtattgg
    16561 tcgcatgttc atgtcgacgg gctttatcac cgtcacggac accccacagg tactggaaac
    16621 ggtccggacg ttcattgaaa aacgcaatga agaaatgaag cgccaggtgg tgctgaatgt
    16681 ggaaattctg agtatccgga aaacccgtaa tgagcaggca ggtatcgact ggaacgcagt
    16741 cttcagcgac agaacgctgg gactgagcct cggcagcaca ttcaccagtg cagccagcga
    16801 taccgtcact ggtggtgttt ccattgtgaa cgggaaactc accggttcaa aagccttcct
    16861 gaaagcactc tcttcccagg gagatgtcag tgtggtaacc cggaattccg ccgtgaccaa
    16921 aaacctgacc ccggtaccga tgcagattgc caatcagcag agctacattg agagtgtgac
    16981 gacggatacc accgccaatg tcggctcatc cacctccctg aatgcggcca ccatcaccac
    17041 cggttttaat atgacccttc tgccgttcat tctcccggac tcacagacac tgcagctgct
    17101 ctattccatg agtctctccg acaaaccggt tattgagaat tacgagagtg gtggaagcaa
    17161 agcccagctg ccgaatgtgg atctgaaaac catcaaccag acagtggacc tgaaatccgg
    17221 gcagacagtc attatcagtg gcttccagca gtcagggcgt cgcagtggaa aacagggtgt
    17281 cggcacaccg ggattcttcg gtcttggcgg tgggatcaac agtgaaaacg acgacaccat
    17341 tctcgtggtg ctgatcacgc ccaatatcat ctgatttgca gaaggaggac tatcgtgaca
    17401 gatatttcac cgacagagcg acaggtgttt ccgctgacgg caggacggaa tgtatttctt
    17461 gccggaatgg actggaaaac actgcccgca agccataaaa acccccggac gtttgcccgc
    17521 agtctggggg cagtacgcta tatttcctgt gagtatctca gtacggaaga taccgatcgt
    17581 cacatcatgg ttgcagccgt cagccagaat acaatcccca aaggcagccg caggtatttc
    17641 tctctcgcca tgcttatcct gcctctgctc gagccaggag gatacgctat cgttgaactg
    17701 tcacaggcaa acgatacaga actttatggt tttgtttctg ctgttgacgg catcctggtc
    17761 agtgacctgg tgggaacccg ggaggaaatc agggaggccc gggaaatatt cctcaccatt
    17821 aacagcgcgc ctgaacacgg ctggacatgt tatgaaccgc cctcgtttaa cgggcctgac
    17881 ggcagaggtc ccctgccact ggaaaccctg acaggtgcag ggaaatatcc ggcagaagca
    17941 cgccttcacc ccgtatcccg tgggccacag cttattccgg tattactcat actcaccctt
    18001 ctgggtactg catggtatgg ctggcagtac tatgaacgcc tgaaggcaga aaaagcagca
    18061 cttgctgaag cggcacaaaa agccgcagag gaacggatca caccaccgtg gctgagtatg
    18121 ccggaaaccg gagattttat ccggggatgc agtgacgtat ggtcacgcct gccggtgtcg
    18181 gtcgccggct ggcgatattc actcgccgag tgtgctgcca ccgggaacag cggcaatctg
    18241 cgtgcatcat atacaaacat cgccgggacg acagtgaccg cattcagcca gcgggtcagg
    18301 gaaattttca gcatccggcc gtttatggtt atgccggacg ggaatagcgg tggttttgcc
    18361 ctgccggtca ccttcagtat gcctgaaact cccgttgccg ttgaggcatt accggaaaac
    18421 accctcctgc aggaacggct gaccacactc gcccggtcga tgcagctgaa aatggactgg
    18481 caggaagtca gcaacagctt caccgatgaa gacggaaata ccatccagcc cccctggaag
    18541 gagtatgacc tgcagatact gacgacactt ccggcgcacc aggtggcaga gcatttcagt
    18601 gaaccctccg tccgttttat ctccgtaaca aggcaactgg aagagggtcg tttccgttat
    18661 caattcacag gtaaatatta tgtccagtaa aacaaaagct cttctgcttt ccgtcctgat
    18721 cctgccgaca acggtcgcgg ccagcaccat cagcaacatg caacagaata accggcaact
    18781 ccaggtggaa cagattcagg aagaaactgc actgtatgaa gccatgctgg caagagaaaa
    18841 ggtgcgacat gaactcagaa ccctggagca gggagaacca gctgagcctg tgcattcaca
    18901 ggaaagcagt tcagccgtca cgacagatac ccctgccccc attaccggta ttccccggct
    18961 gacagagata tccggagttc acccccagat aaccgccagg atacaactgc cgggcggaca
    19021 aactgctgat gtaaaagaag ggcaacacat tcccggcacc acattccgcg tggaaaagat
    19081 caccactgac agtgttgtcc ttgaacaaaa tggtatcaga acaacactac aaccctgact
    19141 cagtgccgcc atttacagcc ggctgtcgga atagattatt cccccggccc ttaatcacaa
    19201 aaaaaatcgt ttccgggtac cgctgcccgt aaggagattt tatgctggca cctgcagacc
    19261 agccacctga tgacattctg gattacgtgg ttgttgatac agataaaaca ggacaaattc
    19321 atgtcagagt ggtggaagga aaaaaacacc ttcgtgccgt ccaggattat ctgacccgcc
    19381 tgtgctcccg ctatccgggt aaagtatccc cgtttgagtt caccacactt gatgtgatcg
    19441 cccgactcag gcaggagacg gtcatttccg gggtggaaaa cggaattaac cccgtacagc
    19501 aaaaaatgct cgggtatatc agccatgcgg ccaccctcag cgcctcagat cttcacatca
    19561 cgccggggcg tgacagtacg gatttcacgt atctggaggc ccgtgtccac ggagagctgg
    19621 agctgctcga tatcgtcaga aaagatgagg ggctggaatt actgggggcc acctattccg
    19681 gaatgactga cgtcataaag ggaacacagt ttgatcccgg tgtcccccag gatgcccgtc
    19741 tggctgaaag attcctgaaa ctggccggac tgtttggtgc gcgctactcc cactatccgt
    19801 gtgtgggcgg actctatgcc gtactgcgtc tgatcaagga tgacagtcag catataccga
    19861 cattcagtat gctggggtat caccctgagc aggagcgcgc tgtccgaagg atgctgcagc
    19921 gaccggaggg catcgtgatc ctctctggcc ccaccggttc cggaaaatcc accacgctga
    19981 ggacggcttc tgccgcctat ctggaacagt atggcttcaa cgataccgga gggatcctgc
    20041 tcccccggcg acgtctgttc acaatagaat caccaccaga ggggcgtatc cccggtgcaa
    20101 ttcagacggc cgttatggat accgctcagg gctgggtcga ttctgttaag tccgccctgc
    20161 gccttgatcc tgacgctatc ctgaacgggg agatacgtga ccatgcctcc gccatcacgg
    20221 cgataaaagc cgccatgacc ggtcacctga tgctgaccac ccttcatgct aacgacccga
    20281 taaatattct tgagcgtctg gaaatggagg gcgttcaggc aaggatgatt gcagatccgc
    20341 agttatttat cggcctgctc agccagcgtc tggttcaggt gatatgtccc cactgccgcc
    20401 tcccctggca tgaggtggag agctcccgga cggatgaaga acgacgcctg gtggaaaact
    20461 tctgccagcc cgatgccgtg tatcttcgta accataacgg ctgcccacac tgctggcgtg
    20521 gagtgaacgg ccggaccgtc attgcagaag tgatttcccc ggatgcgaag tttttccaga
    20581 tttaccggga aaaaggccgg attgaggcaa aaacctactg gcaccgggag ctcggcggta
    20641 tgacacgaaa tcagcacctg ctgggcaaaa tcaacagtgg tcaggtcgat ccgctggctg
    20701 cgcattacat atcccctgta gacgaggaca gctatacgct gctgcactga tatgaaaatt
    20761 ctggaaagac tcaacaatac ggaactggaa ctgtccggcc ttaaccgctg gctgggcaaa
    20821 aagacgttca ggcggactac gttttacgag tcccttgcag gcatgatccg ggacggcaca
    20881 ccggttatgc gggcactgga gttcatctgc gatgtggaaa cggattttgg taaaaagaaa
    20941 gggcagtccg gcctgtattt tctggcgaca gactgtatcg ccagcatacg cagcagtggt
    21001 cagctctctc cggccctgaa ggactgggta cccaaagatg aaattgccct gatccgtaac
    21061 ggtgaagaac gaggtgatat cgctgaagcc atgtttcagg tcgtcaaaac ggcaaaagga
    21121 cgacaggaga tgattagcag tctggtgagt gtctgcctct atccgctcat cctgctgaca
    21181 ctctgtgtgg tgaatatgta taacgttcat acccgggtta ttccgatgat cagcgccttt
    21241 gcaccggaag aacgctggtc agtacagatg gtcattctgg ccaaatcctc cggactggtt
    21301 atcggttatg gtttgtggct gctggcagga ctgctgctgc tggcaattct catcaggatg
    21361 tcattcagtc tgtataccgg tccccgacga cggtatcttg atcgcatccc gccatggtcc
    21421 ctgtatcaga ctttccatgg cgtgaattac ctttttaata tttcatccat gctgcagata
    21481 aatattcctt tatctcatgc ccttaccaga atagaaaaac atgcagaaca taaccgatgg
    21541 ttaaaatcac gaataagtgc tatcagaaaa aatgtcttat ccggtcagtc actggcactg
    21601 gccatgaaaa actgtggtta tgactttccg tcaaaatcct gtgtgaataa tcttctttta
    21661 aattcagaaa gggaggacag tgttgccagt atggcgcttt atgcagaccg ctggctggaa
    21721 gaagcaaaaa agaatgtcag aaaaagcggc attattatta ctgctatatc aggtctgctg
    21781 gtatttctct tcatcatcaa tatggtcagt gccatttacg gcatcagtga catgctgaag
    21841 cagtaaacac agcatccttt atattcccct cgatccaaat tctgttttac caatctcagg
    21901 agctaatatg agcgtcataa cctcttcgta ttcagaacat aatattcgtc accttcagaa
    21961 cgatgataaa ggcatgaccc tgctggaagt tctcggtgtt ctggtcgtcg ctgccattgt
    22021 tattggtgcc gtgatgggac tgatgtccga taccctgtcc tcttcagaca accagaagga
    22081 actcaaaaac ctgcagacaa tcgccaccaa aatgaaagcc caaaaattcc aggggcagta
    22141 tacaggtact gactacgtca aaatcctgac cgaaagtggt ggcctgcctg ccgatatgat
    22201 cgccggtggt aataaggcga aaaatgcctg gggtggtgcg gtaaccatca aagtcagttc
    22261 agataaatac tcctacgtta ttgaatccag caatgtgccg aagaaaaact gtattgatct
    22321 ggtcaccagc ctgcgcagtt catccatgtt tacaaaaatc aacggcaatg tgacgaataa
    22381 agttgacccg tctacagtat gtaacgcaga taaaactact atcaaactgg aaaccaacag
    22441 ttaatcattc atcagcgccc gcccgggcgc tttatttaag gtcactgtat gaacaggtta
    22501 tttatcctga cagtgatttt ttcactgtta tgctcacttc cttccgcaca ggcatcccca
    22561 caggacaaac aatatgatcg ttgttttatt accgccggaa aacgttattc cattgatcca
    22621 ggattactca aaattattgc ccgcagggaa agcagtctga atccccgggc cattaatcat
    22681 aacaggaatg acagaggaaa aattatcagt accgattacg ggataatgca gattaactcc
    22741 acacatattc ccgggctgaa aaaaatgggc attatccgga gtgagaaaga tctgttgaat
    22801 aatgtctgcc tgaatattca gaccggagca tggatcctcg cccggcattt tcagcgatgc
    22861 ggcgtaaact gggaatgcct cggcagttat aacgccggct tcagtaaaag taacactcac
    22921 cgccggatga aatacgcccg gcagatttac agcgcttaca tgcagggacg ttagtccggg
    22981 agaactgcca tgctccagat aatcccggca ctgctggtcg ccataaccgt ggtaatacag
    23041 tgtctggccg ttcagatgag tcgctatcga ctctcccgga tgaatgcccg cccccctcac
    23101 catgggctgg cgctgatgag cgggatgctt gcagctgccg ccattatcct gcgtctttat
    23161 cagaatgaca gcgctgtcgg ggtgctgttt atgacgataa tgaccggatg tatgacactg
    23221 atgaccctga cggatgctgc atcctgccgt cttccccggc ccttcaccat catgtatctg
    23281 ataagcggca gcgcatttcg tctctggcag ggagactggc tgacaggcct gtgcagtgca
    23341 gggttctggt tcctgctgtt gcttctgttc aggcaataca cctcttaccg caggaaaaca
    23401 gaaaccatag gcctgggcga tgtttttctg attgccggta ttgccatctg gtccccgccg
    23461 ggagacatcc cctggattgt cgcaacggca gcaggcggag ccttacttta tctgtggtgt
    23521 aacagacaac gacgcattac cagagaactt ccctttgggc cgtttctctg cgcgtcacag
    23581 tacgcattta ctttttatcc cggtggcctt tggtaatacc ggagggaacg actatgcaaa
    23641 aagacaacga taaaggtttt gcacttcttg aaattctggg aggactggtt gtcatcagcc
    23701 tgctcatgcc gctgttctgg tcttacattg aagactacct gaatgaaatg cgcaaccaga
    23761 gcgccgcatt tcatgcagat gcctacaata ctgcagccag gacctacatc gccgataaca
    23821 atgcccgcct gcatagcgga acgcttcctg ccaccttcac ggcggatgag ttaatccgga
    23881 aaggatatct gaagggtctt aaccgctcgc cctttgggca gagttatacc accggcatcc
    23941 gtcgtaacac gagtaccggt cgtctggagg ccctgacctg ctccaccgga ggggagaata
    24001 tcaaagacga tgccctgcgc agtatcgcca gcctgctccc cggtctgggc ggatttatag
    24061 gaaaaaatgg cacagcaacc ggtgtttttg gtggatggac agataaaccc ggcgactatg
    24121 gactcagctg caatggcggg cacatcgcca tcgtaatgat gggtgatgac ctgcaggaaa
    24181 gcgatcgcct gtatcgcttt caggtaccgg gacgaccgga gctgaaccag atgaacacgg
    24241 ccatcaatat gggagggaac aacctgaaca atgcaggaaa cgtcaatgga caaagtgcaa
    24301 cactgaaagg tgatgttaca agcgaaaacg gctggttaat caccaaaaat gataaaggct
    24361 ggaagaacat cacctatggt ggcggtttta cgatgacaga cagccagtgg atacgcgcag
    24421 tcggtggtaa aggaattata accacagggg aaatcaaagg aggaaaagtc agtgggggga
    24481 cagtccggtc tgacggtcgc ctttccacag gtgagtattt acagctggat aaaacagccg
    24541 tagcaaatac aaagtgttca ccagatggac ttgtaggccg cgacagcaaa ggggcaatac
    24601 tttcgtgcca atccggtgtg tgggttggct ttgaatccta ccctgtggga agccctatcc
    24661 cgtggccatc tgccactcct ccacaaggct atctgttgat gaacggccag tcgttttcct
    24721 gttcccggta tcctcagctt gcccgtgctt atcctagctg taagttaccc gatctgcgcg
    24781 gtgtttttat ccgtggctgg gataacggga agggacttga tggtgatcgg aacagacagc
    24841 tcctgagtta tcaggccgat caatcaggcg tctatcacga acgtggcgga tggctgaaag
    24901 ggcatcatag tggtatgcca tactgggcac aggggagtac cacagaaatg cgtcccaaaa
    24961 acattgcatt caactacatt gttaaagcca gctagggact cacatctgat aggtatacac
    25021 agagaaaaca ccgttaccac aggcgtaatt ttgtgttggt ctggagatga tttgatatga
    25081 cgctcccact ggcacggcaa atgaaataaa tctccgggca tatcagctgg tcagcaaact
    25141 cgatgccagg cgtaaacaga ccagcaaaat ttccccttat tttgttccct accctgcaac
    25201 tgtcgggtgc agaaacgggc tgtttgtcgt gacactgcac gatttcgacc ttgaaacccg
    25261 tgcagaaacc cggggactgg caatatctca tgccagcatg ctcagtcacc tcctgtccga
    25321 atccttttca ttcgccctta aaacagagtt gcaaacactc ttgaaacaca ctcgatcata
    25381 acgatcgaaa taactcgctt ccgtttaaaa aacactctct ttagactcaa tttatgaaca
    25441 aaacacgctg acccgcatgg aggtgaaatg aaaactgaca gcacaggtat tgccgcccgt
    25501 atgatgctct ccctggacag ggagcgaatt tgcgaatgcc tgctcagtca tcgccagctg
    25561 cagtccacac ccctgcaggt tcgttatccg cagggagtca gggatgccct tggtatcatg
    25621 agcgaacagc tttctctttc cgtctctgac ctgacgcgta tcctggtcga agacgcgtta
    25681 agtgaaatgt ttcttcctgc agataacatc gtccgccgtc ttctcagcag gatggagcac
    25741 atcatgcagg cacatgatat cagcgcaaca acgatggctg cattactggc cccctggaat
    25801 atccgccctg ccgtcttccg ggagccggac agactcactg actatctgac cggggagatt
    25861 cttgcagcac tggctgactg gttttatctc agtccggaat ggctgaacgg cagggtgcat
    25921 tatccactgt atcgtccggg agactggcca gccacacagg agatattctg caggattatt
    25981 tcagcccgcg agaatatgga cattattctc tggcatggtt ttccgtttgc cggaacgcat
    26041 tccggagaat attgcggcgt tctgctcagg cagaaaaaag aaattaataa caccattatt
    26101 tatccggtac tttcgttata tccggccaga atggatatag aaaaagaagg ctggtttcag
    26161 atggcccgaa aaatatctcc ggatatccct gttcgcgccg taacactcac tccggcacag
    26221 gcagaatatc tgataacagg aaaaatatta cccactgcgt tattcagggt tccgttatcc
    26281 ccctggtaat aatactgagc aataaaccgg aataatacac caactgtttt taatggcaca
    26341 gccgtgccct ctgttgttat acccaaaata cctcaataca ggaaactgaa atgatgatga
    26401 aaaagcatat tacccgtact ctcatcgcgt ctgccgtttt attttccttt aattcgactg
    26461 cagcaacgtc atattttgaa gcccgcaatg atgcgatggg aggaacgggg gttgcctcct
    26521 ctcactatgg tgtggcccca ctggcaaacc cggcactgct gacaaaatcc ggtgcaaaag
    26581 atgacttcag tcttctgctt ccgtccgtgg gtgcgcagct gtccgatccg ggtaatatcg
    26641 ccgacaacgc cgacaaaatc agcgatgact ggaaagcctt cgaccgtgcc gtcgacagcc
    26701 actccggggt accgcagaca gcggcccgcc tgaaagagag actgcaggac ttccggaaca
    26761 cccatgccag cgcacaggcc ggggtatctg ctgtggcggc ccttcccggt gataccctcg
    26821 ctgctgcact gatgctgaaa acgctcggga ctgtcagcgt cgacggtaaa gtcagcgatg
    26881 ccgacctgaa ctatctggaa agcattgcag acagtggcag ccaggatgtg gataaaaaca
    26941 gactgacctc acaggctttt gcccgtgccg cactgatcac cgatgtgggc gttgcgctgg
    27001 ccactgaact ggaaacggcc ggacaaaaat ggtcactggg cttcacacca aaattccagc
    27061 gtgttgacct gtttaactac aatacgctga tcaaaaacta tgacagcagc gcattcaaag
    27121 gtgaccgtta ccacaacacc caaaacggta ttaacgctga tatcggtgcc agcatggacc
    27181 tggatgacaa ctggacgctg ggtctggtcg cacagaacct gatcccacgc agcattgaga
    27241 ccaaagaggt acatggtgtc actgaaacct tcaggatccg tccgcaagta acaaccggcg
    27301 tatcatggca caacgacatg ttcaccacgg cactggatgt ggatctgacc ccggccagcg
    27361 gcttcacatc agacagcaaa cgtcagtttg ccgccgtagg tgctgagttt aatgcctgga
    27421 aatgggcaca gttacgtacc ggttaccgcc agaatctggc cagcaacaac ggcagtgcat
    27481 tcactgccgg gattgggctc tcaccgtttg atgtgattca cattgatgtc gccggcctcg
    27541 tgggcaccga caacaactac ggtgccattg ctcagttaca gttcaccttc tgattatcac
    27601 gccccgggac gcccggggct tctgacctga atctgctcgt ttccgctctt ctggccactc
    27661 accgccagtc ctgttcatta ttttccggat aaaaccatgc aaaaaaaacg tatcaccctt
    27721 gcagggggag ttgccatgct ctgtctgagc ggctgtcatc ttcccccgga taataccgca
    27781 ccggccagga agtacggtgc agcttctgaa gtcgctgtca cacgacatgc acagtatcag
    27841 ttgtgggagc agggcacgta ttctgcagcc ctgccttccc gccagcccgg aggcagcctg
    27901 caggctcaca gcgggcgggt ttcctttgac tgggaaggcg atgctctgga gctcctgagc
    27961 gagctggccc gtctgcgcgg acaacagttc agttacagcg gcgtgcgtct tcccctgccc
    28021 gtctcactcc atgtacgcga tatgaccttc gctgatgccc ttcgtctcac agaggcccag
    28081 actgcctggc gggcagtcct ctcacagtca gccgggcgtt tacatctttc ctatatgcca
    28141 acggagaaaa aatgaaaaaa caggtctgtc tcctgctgtc cgggctcctg ctggcaggct
    28201 gtgccggaca gcaccatcgc ccccctgccg gtatggcagg caatttacca ccgccggatc
    28261 tccgggcatg gctgacgccc actgacaacc aggatgacgg catttcagac acccgtcgcc
    28321 agatgctcac ggacgccgga cgaacgctgg gattccgtgg cggaaaagca cagcgctcat
    28381 gggaactgat tcaggcactg aatgcccggg aaagtacact gaatgccctg tatgacttcc
    28441 gtccccttat cagcagggaa ggctggctac cccctgttat tgatgaagcg caggatgttg
    28501 ctcacattca gcctgaccag ataagaaccg cgtcccgtgt ctggaccatc ctgcgccccg
    28561 aacgttttgt cagtaacccg ccgggctggc gtgactggct tctgcggggg ctttccacta
    28621 ccgccacccc gggaactgag ggccgtgttg taccggaaga ccgcgcacag cgcagactct
    28681 gggaaaacgc actccgccag ggctggcagg aaggccgtga caacgctgac ctcaccctgg
    28741 aagccaacca gaaacgcctg acccgggatt accggggaat gatgctttat gccctgctct
    28801 ggcgtcaggg catgatcaca cgcccggatg tgactgaaca gcggcagacc gtcaccggta
    28861 acggcaggaa actcatcacc ggagaccacg tccgccgtct gaagacacac gcggaattca
    28921 ccctgcagaa atcacgctgg cgtccggtcg tcagcacgga aggagccccc gatgaaatta
    28981 cccgatgagt tcggcacatt ccctttcgcc cgtttcacgg ccgaagagtt tcgctatttt
    29041 tttgcctggt gtgcccgaca ccgcgtcagt gatatcgatc tgacaggcgg ctcaccggta
    29101 tctgtcagtc gctttggacg gcgggtacgt tcgtccgcac atcctctgcc gtcctccatc
    29161 atgatggcgc tgagtgatga attgtttgga cgggaagtcc ttccccgggt gctgtccggt
    29221 catcccgtgg accgtaccat tcaggtcagc ggtgatgcct ccggtcgcta cggtctgcaa
    29281 cgggggcagc gtgttcgcct gcgctgtcat ctgattcagg gaacatcagc aacagaagaa
    29341 aaagtaatct ccgtcaccat gcgggtgatc ccgtccgata ttccggacat cctcaccatg
    29401 aatattgaac cggacctgct ggatgccatg ctccggaaaa ccggtctggg tttcatctgc
    29461 ggagagaccg gctccggtaa atccacactg gcagcggcac tgtaccgcta cattcagacg
    29521 catttccccg accgcaaaac ggtgacctat gaagatccgg tggaatacat tctgggggga
    29581 gagaatgacc ttctgccacc ccatcaggcc gaaatcgggc gtgatgtgga aagttttgcg
    29641 gcaggacttc gctctgccgt tcgccgtaac ccggacatca tcggtatcgg tgaaatcagg
    29701 gattatgaaa cggcagatgc agccgtccag gcaggtaaca ccggtcactt ctgcatcggc
    29761 accatgcaca ccaaatcacc cggtgaaacc tttgcccggc tgctggggtt atttccaccg
    29821 gaaatcaggg attcaatggc agccgccacc ctttccctgg tgcagttcat tctggtgcag
    29881 gtcctggtca gaacaaatga cggtggcaga caggccgtcc gggaatacat cgtcataact
    29941 gacgaactgc gtgatacgct ctcccggcag tcccatgcga catgggggca ttacattgat
    30001 gaaatcattc gcaaggaaaa acggcggata cgggatcagg ttctgaccat gtatcagacg
    30061 ggcagcattg aggcatcaga agctgcgctc ttcatcccgg tcggagaatt ccccaaatga
    30121 aacgcgaagg cttctggcgg gatgtcactc cgccactgaa tatttatggt gtcccgttgc
    30181 cgtttaccat gatttacctg ctgctgtttc cgtatccgtc aaaaacaaca ttctgggtgt
    30241 gcagcggcat tttgctgttt tttgtgctcc tggggtactc aggatggact gtccggacgc
    30301 tctgccggcg tctgtacagc ctggtgcagg gtaaacaggc gtcaggccgt ccctggtggt
    30361 accggcatcg cacagaatta ccccgtgaat ggcgcggaac ggaataaccc tcttttgttc
    30421 atcatcccgg cgtatcacaa cgccctcatc tttaatcccc ggagaacgac atgaagtcca
    30481 gatatcgaac ctggcttgct gtcccgcctg aggagacaga agccgtaaaa aatgcagttc
    30541 ccccgttaaa tggccgcaaa gctgtcgcat gggatccaga aaaaaagctc tggtatgccc
    30601 gggcgggtac cgaactttca ctgctggagc gctggttacc ccggccacag gaactgtcga
    30661 tggatgccgg cgatccggtc actgaatttg ctcaggtcct ggaaaacgcc gggctggtta
    30721 ttcagggatt gccacagatg gacggagcca tacacagagt ggccacccgg gatgacaaaa
    30781 aaggggccaa aagtggcgca tacagggcct atcttgatgg tcgtcccgcc ggctggtacc
    30841 gcgactaccg gagcgccgat gacagtccca caaactgggt attttccggc ggagaacaac
    30901 acgatcctct ggccagactg catctgcggg cattcgctca gcaacagcgc gatgataacg
    30961 ctcgtaaact gcagcaacag tataacaaac aggccggcta tgcccgctcc tatattaaca
    31021 ggttgccaca ggcaacagca catgaatacc tgacgcgcaa gggcatccgg gccgcgcctg
    31081 gcgtgcggct gaacaataaa aatgagctgg ttattccctt cagcaacggc cggggagaaa
    31141 tacgttcgta ccagcgtatt ccggtcaccg gcgggaagga tgcccgcatt ctgaaagact
    31201 ccgagaaaac cggcaactgg ttcaccttcg gaaccccgga aaatggccgc cccctgttat
    31261 ttgcggaagg ttacgctact gccgcctccc tgcacgaagc aaccggcctt ccggttctga
    31321 tgacagttga tgccagcaat atgattgccg tggcagaaaa tgcccgccaa atctggactg
    31381 acagcccctt tgtcttctgt gccgacaacg atcatcaacg ggaaattaac aaaggggttt
    31441 ttagtgcaac aaaggccgca gaagtcacga atggcgaagt cattattccg gcctttacgg
    31501 aggcggaaaa agcacaggga ctgaccgatt tcaatgacct ggacgccagc cggggacggg
    31561 acaactttca gaatgccatg aatgcacagt taaaacatat cggtatcccc acacccaaca
    31621 gtgacactgc cgaccatcgt gaggctgtcg tgatcgacaa cctgatattc acaccggtaa
    31681 aagatgagaa gccacaaatg tcccctgaaa acagacagag cacagccccg gaaacggagc
    31741 tggcgacaca ggatacgcct tatgacaccc actccgacga tacagaaaac caggcatatg
    31801 aagatgacta tgccgcctat gcatccctgc aatcagtaac tgcggagacg ttatccccgg
    31861 ataaaccact ggagaatatt gcgtctgccg ctgataccgg cacagagaat acctctgtgc
    31921 ctaccgctgg cgaccaggaa gacctcaata cagacgccac aggtacagac accgctcaca
    31981 acagcgacac agcagaaaca ggaacgcttc ctgaacctgc cgggatgcag tccgtgcagc
    32041 aggcaaccac tgaaccgacc gcagaagata aaccacacga gaacgttgtt tccgctgccg
    32101 ataccagaga agaaaatgcc tctgtggcta ccgctgatac cggtgaagaa aatgcctctg
    32161 ccaccaccgc tgacaaccag agcacgcagg cagaagaaca tccccggagt aaagacacac
    32221 cggaaacctc cctctccgac gcggccggga cccagcacac caccacaacg cagtctgaag
    32281 aacaagtgca ggtttccatg gccagcgctg acgactggag cgcatttgcg gaagatctca
    32341 acgctgacac tccgggggcc cccactgttc acaacaccga caacgcggag acaaacacag
    32401 tttctgaacc ggccgggatg cggccagttc agcaggtgac cactgaactg acggcagaag
    32461 ataaagcaca ggaaaagagc gttgcgtctg ccgctgagac cggcgcagag cactcctctg
    32521 acatcaccga tgacaacagg aacgcacccg ctgaggagct caatgccgat gccacggttg
    32581 ctgttcacaa caccggcaca acagaaacag cctcaccttc tgaacctgca gaaactcagc
    32641 cagtccggca ggccaccgct gaaccgacgg ctgaagataa actgcaggaa gagcgtcttg
    32701 tttccgccac tgagaccagc acagcgaatg aagcagcccg ccctgaaacg ccatcgcttt
    32761 tcagccgtct gcgccgtttt ctgaccggtg tgaaatcacg cacctcagaa ccttccgtta
    32821 ttcaggagaa aaagcccacg gaggctgaaa cagcaacaga ggaggtcgca ccaccatcac
    32881 caccggaaac cagcccggaa gacacatcgt cagcggaacc ggacacgatt atctttgcac
    32941 cccgccgtcc cgacagtccg cagcggcaca acctggacga gatccttaaa aatcttacac
    33001 acaaagagtt tcccgacagg actgcgctct acagcgttga cggtgaaccc gccttcctgg
    33061 acagactcta ctgtctcgaa atggtggatg gcgccagcgc ggatgacaaa aaggtcctgg
    33121 ctgcccttgc tgtggccacc aacttctatg gcggcgttat cgagctgacc ggcagtgatg
    33181 cctttaagca aaaagccatg cagctgattg tcgaatacga catcaaggtt cgcatgaaac
    33241 tcccggccca gcgggcagca ctggaaaaaa tccgcaagga aaaagagatg gcgcatgatg
    33301 ccattgtcac ccacagaccc acgccggaac tcaaccgcca gtcaccggaa gagacaacaa
    33361 tggcccaacc ggctccccct gtgtcaccga aggcagaggc tgaacaaaca gcaggcagta
    33421 cggctgaaac accgcatccg gaagcgccgg tcacggcaaa agctgaagcc ccccgggccg
    33481 acagaccgga acaacccgcc gccccgtcgt ctgcgccaga aaaagcccct gtcagtgcac
    33541 agccgcagca acccgacaca ccggaggaag agccgcccgg caagctcagc cccggtgaaa
    33601 gtgtgactgc cgtcctgaca aactacggag aaaaagagta tgcgcccgga aaagggcgct
    33661 gcttttttgt ggaactcacc aaccgcagtg gcaaacggga atactgggga aacggcctgg
    33721 aagaactggt caaaaaccat cagaagggtg accccgtcac actgacactg caggcgagag
    33781 aaacattccg gctgtccggt gaaacgcggg atcgtatccg taatatctgg gccataaccc
    33841 cggtcagtaa cggcattacc gttaataatg accaacctca tgcgggtcag cataccctgg
    33901 agtttccgat ggcaacattc agccaggtga tgttacagat gcaacaacac tggccacaga
    33961 taatgcagga agttcgctcc cccaaaaatc ctcccaataa tctgtacatt ggtgaagaca
    34021 ggcaacctgc atcagcacct caggatcaat caatgcgact ccccctcgct ggtaatgccc
    34081 ccaatgaact gataccagcc atcgcctctg ccgataagga caatcgtcag ttgaatcttc
    34141 tgctggtcca cagcgcagat gatcatctgc agggcgtggt ccgcctgaac ggcacccttt
    34201 acccggcact ggccaccccg tcagctgaca accgtcagct ggttatcaat gccctgacgg
    34261 ataacggtct gcagtttgcc ggttacggtg aagctgttaa ccatgatgag aataatcatc
    34321 agcgtccgtc cccacaaata atgcagtttc acctgaagca acaggactcc ccgttgtttg
    34381 ccgccatcca caaaccggaa gagcaaccgg acaagctttt ccgctctctg ggttttgagc
    34441 agacctggaa agagtggtct gacagccaaa aggcggagga caggcaggaa aaaactctgc
    34501 aacaggcaca atcccacagc cccggccgat gagccggtaa ggacgaccat gaacatgaaa
    34561 aaaacgctgc tggtcatact gaccggcatc accctggcat caccggtggc aggtgccgcc
    34621 acctgccggg cgggggccgc tgccgaagcc ggcagcagag ccgcctggga gcgggcgcgc
    34681 acagccagcc aggcatggtc acagcgggag aatgacgtcg cctcctccct gcagtcctgc
    34741 ctgtcccgca tcaaggacat cagtatcact cttccgcagt tccccagcct ggacaagctc
    34801 ctccgcgacc tggaaaacca ggtctgtgat gccgtcgtgg atcgggtgaa tgaccatctt
    34861 ccggggaata tcgacccctg gaaagactac aacctctgaa caggagcacc atgagcgaag
    34921 cacaacccac ctcccctgtc tcaccggaag cggataacag cggagatatt tataaccccg
    34981 tgctcagaac catcgcacag tcagaacaac gggccgcgct ggtacccggt ctggtcaggg
    35041 cgttgctgta cagcggcgca gcactctgca tcagcctggt ggctaatggc atacagtact
    35101 ggcatgccac cggcgtgacg cgcgaatact ttgccactga caatggccga ctggtccgtc
    35161 tggcaccaac cagccagccg gcctggagtc agagcgacgt catgaacttt ggcagcatga
    35221 cgctgagcga agccttcaat ctcgattttg tccactaccg aaaacaaatc acgacacagg
    35281 ccccccgctt ttccgaagcc ggctttgccg gctatgtgaa tgcccttcag gcctcgaaca
    35341 ttctcgacac tattaaaaag gagaaattta acctcacaac cactatcggg gcaggtaccc
    35401 tggtcaacca gggacagatg gagaacggcg tctggttctg gaaattccag tatccggtca
    35461 ggatgcgcct ggtcggtcag accagcaccc gccccgaaca ggccttcacc tttgaaatca
    35521 ctgtccggcg cgttgacccc cgcatcaaac ccggcggcat ggaaatcagc cagatggttt
    35581 cccgtaacgc cgcagcagga atctgagact catatcatgc acatacatca tcttattggc
    35641 gcactgctta ttgtgtgcgg cactgtcaca gctgcagata acccgggctg gaaaaatgca
    35701 cgaagcccgc agaccggcgg aaagaccgca caggcaaccg ggaacacccc ggcaggcggt
    35761 cccccgccag ccgcacaact cccgggaaac ctgccgcctc ccggcgctgc tcttgtacag
    35821 gaaggcgcat cgctggattc tcccctgaat gccagtgaaa ttcgtgaact ccgtgggata
    35881 atggccgata acgaacgggc catcagtgcc ccgctcacca gtgtggtgcc ccgtatcagt
    35941 tccctcacgg tgaatctgtc tccgggggcc agcctgcccc ttgtccgtac cgctctgcat
    36001 aacgtcagcg tggtgacctt cacggacgtc aacggcagtc cgtggccaca gtcggatccg
    36061 ccctataacg ctgcgcccgg tcactttgac gtgcagttca acggcaatat ggtgtccgtc
    36121 acccccctgc gcgcctgggc tgccgggaat gtgacggtct atctcaaagg attgtctgta
    36181 ccggtcatcc tgaatatcac cagtggtgaa acggacacca ctaccctgag tcgggaaatc
    36241 gacagtcgtc tggatctgcg cattccccgg cggggacctg ccagcccggt ggtgtccata
    36301 ccgtcagaaa aaattgccct gcatgacgcc actctgcagg cctttctgga cggtgtgcca
    36361 ccggatgatc ccgccatccg ccgcctgaaa ttctccggta acgtcccgga caccaccatc
    36421 tggcaacacg gcgatgatct tctcgtgcgc tcacgggcca tgctgcggga tgaatttgaa
    36481 cagacgctgg catcggctga tggtacgcgg ctgtggaaac tgccggtgac ccccttactc
    36541 accttctccg tgaatggtca gtccgttcac gtcaccccgg aactggagta aatcatgagc
    36601 gcagaacaag aacaggacac agccgcaaaa tccggcaaaa agctgtcagc cctgctggtt
    36661 atggggggcg tgattatcat cgggggcgga tacctgctgc tcacctggct gtcccccgaa
    36721 aacgctgcat cgctctcgtc agtcaatatc aacagcacaa cgtccggcgg cggacggaac
    36781 gtgatggaga cacagcacta ccgggattta ctgcgggctg acaatgaacg tggtgctgca
    36841 gaagcgttac gtaataacga gacgtttatc gccagtctgc cccggggact ggataccgat
    36901 accggaaacg atcagaaaag ggataatccc cccgatgatc agtcaggcac ttccgggcaa
    36961 aacgcatccc cccctcagga agatcagact gcccgggaaa aacggcgtga ggcattacag
    37021 aaactgcttg cccgtatcaa tacacagcat caggatgccc ggccaccggt agtggcagca
    37081 gccatgtgga gcaccaccgg cactcccggg acagccccac atgcgtcacc gtccggtacc
    37141 gttacatcac agcatccccg tctgcaggag acgagtctgg caacaccggc tacacgtcag
    37201 gggatccagc tgataccggc actgacccgc gtaccggctt acatcgacac cgaagtggac
    37261 tctgacaatg cggtctcccg tgtggtggcc tccgtacctg ccggcccatg ggccggggcc
    37321 gtcctctaca gccaggacgc cagactcgca ggtcagggaa tggatatcca ctttgaccgg
    37381 atgatgtggc ggggcatgtt tctgaaggtg aatgcatatg ccctgaatga acgaacagga
    37441 atgtcaaccg tggccagcga cgtgaaccac cgctggttta agcacatcgt tctgccgtcc
    37501 gtacttggcg gggtgggcaa tgtcggaagt ctttacaaag acgccaacac acagatcctt
    37561 cagggaaact acggcagtat taccggcagg gtgggaatgc ctgatggaga ggcgctggcc
    37621 ggcgtcattg caggtggcat ggccgaacgg ggcagccagc tgctgacccg ccaggcagag
    37681 agcgaacctt accgtcaggt gacagtatca cggggcgagg tcgtctccat tctgtttgtt
    37741 gagccggtca tgagcaacga tatcagtacg ccgtcctccg gcactgcccc ttctgagtcc
    37801 ccctcctccg gacgtccgct cacccaggcg gaggcagagc accaggcctc tgacaggatc
    37861 cgggcggcgg tcctccgtaa acagaatgaa atgcagcaac gctacggcat acaacaggaa
    37921 agttatccat gaaaaacgaa catgaagatc ccggcagctt tggcgaaacg acggcaccgg
    37981 catccggcaa atccttcctg aaacgccagt atatgggata cagcctgccg gtctgggccg
    38041 gctgtgccct catcatcctg gtcgccatct ggtacaagtt tctgcggaca ccctcagagc
    38101 cccctgcaga tgcaggcttt gctgtcacac cgcccgctgc tgagagctgg gcagagccca
    38161 tgaccatgac ggagtccggg atcggaaaca acatgcccgc accgccggtt accccacagg
    38221 cagacatgca gcaacttgcc cgtgatctga aagcagagct ggacagacgg gatgaggaaa
    38281 tccggcgcac cctgaccatg ctgaaggaca gtatcaccac cctcagtaac gccattcaga
    38341 aggatgaggc ctgggcacag gaaacccgtc agttgcttga ggagacccgt aagcaggtgc
    38401 ataccctgca gatgcaggga gcactgccag ccccgaccgg gaataacaga cagcctgcga
    38461 aaaccctgcc ttcacctctg cagggcatga aaatcatgtc ggtggagaaa ggcatggcct
    38521 ggatccgctg gcagggcagt gcctgggcag tacgggaagg cgacagtctg ggaaacatca
    38581 ccatcacccg gattgacccg tccagccggg cagtcatcac ctctgccggt acattacgat
    38641 gatgagcatt gatgccctga cagccataga gaactttgcc agcggtattt tcagtgcagg
    38701 catggagttt ctctttacct ggggagagct gctggggatt atcggcctta tcggtcatct
    38761 gatgcgcgcc cgggcagaag gacgtcacag tatgggaccg ggaaaattta ttgccggtat
    38821 cttcatctgc ggcatgctgg tcagtctgcc ctcaatgatc aatgccggtg gtacccagat
    38881 gggcttcagg gcagattctt tcgcccccat cgcatacgtg cagccgggct cctttggtgc
    38941 cgctgccggt gccgtcaatg ccattctctc actggcaaaa ctggccggcg tcggttttgt
    39001 catgaacggc atatccatct ggcgcaaatc actgctggac gggcacaccg ccctgagcgc
    39061 cagtgaatcc atcagtaaag ggaacgtcaa gtttgtggcc ggcgttctgc tggttttcaa
    39121 cgaccgggtt atcgatgcca ctctggcaag cctcggtatc gccttctgac ccctgtattt
    39181 tttcagtaaa tcaatctgtt aataaaggag acactatgtc acgacttcgt gccatggcgg
    39241 cgcgtctgcg cgctgcatct gtttatgttc agttatgcct gatggcctgc cgtgaccggc
    39301 tggtacgcct gttactgctg cttcccgtga tgctgctgcc ccacaccgca atggcaaata
    39361 ccctcttcga tatgattaaa accgttggcg atggtgccag aggctccagc agttcgtttg
    39421 tggctatcgg tcaggttatt ggcctggtgc tctttatcgg cggactgctt ggcctcaaac
    39481 aaatcggtaa aaaacccggt gcagggctgg cctgcctgct gtcaattctg gtcggcgtct
    39541 gccttgccgt cgtaccggaa ctgatgggca gcggacagaa aacgctcggt ctttctccgg
    39601 tttcaatcag ttaatccgca aaggcaccgg gaaaccggtg ccgtcttcac acaatgagga
    39661 ggaccagacc atgtcctgtg agctaccccc tttcaccctg ctcagaacag agcacggcaa
    39721 cgtgattgcc cgcgacaacc gggataatca ggagtttgtg tttgcagagt gttactccac
    39781 cgctatagca gcactgattc tggctgccgt caccctgtat acagagagaa aagcgtatga
    39841 aaaagacccc gccactcatc gccccggtcc gggaacgatg gcttttcccg ctcccgcaac
    39901 cggacacggt attcagcggt gaagccctgt taccaccgcc agtgctctca acagagggac
    39961 gctgcccctg ctgtcgcagg accgtcaccc accgcttcat tctggaagat tcatggccat
    40021 tacagcagat ggctgacacc tgccgggata cgatcgtcct gctggaaaaa aacctcaccc
    40081 gggtgatgcg cctgaaaaaa cgtccggtac cggaaaacgc tgacgaaaaa aagaaacaca
    40141 cccgaaccct gcaggatgcg gagcggtccc tggcacaggc ccgcctgtcg gccagacgac
    40201 tggccctgcg tcatgtggaa aagtcgcaaa tcgtcacaac ggatgcactc agcgaaaatg
    40261 aaagtgacct gctgcagccg gaaggcccgc cctttcatct ctgtgccttc tgccacgcct
    40321 ggcactgtct gaacggctac gctgccgcac agggagtgat ggtctggctc cccgatttgc
    40381 atccggcctc ggtggttgcc ctcaatgcgc gggcactgca ggagattttc agcgataacc
    40441 gtcagcgggt caggcaggga cgggcagtcc tgaatgccct ggtacagaac cgtctggccg
    40501 ttgaggagaa attccgcacc tggcgtccgg cggattttgc agatgccctg cgccgctggc
    40561 cgccggcaca gcgtaaaacg ttgagggaga aaatggacgg ggtggcgctc attctgctgc
    40621 cggattcgtt tcctgacaaa aagtacgtca tgtgacacac accggtgccg gtgacgtttt
    40681 gccggatatc agttctccgc agatgcattc cggcacttta ttatccggtc gctgactgac
    40741 gatattccgc ctgacggaga tgaaaaagag attgcccggt tcattcgccg ccagcagttt
    40801 atttatccgg gtgtaatcac acagaaatac gcccctgccc ctcaacatat tatttatctt
    40861 ctctgctgtt ttttcatgcc acatcccctg ctgtggcatc aaaaaatata agaccttgat
    40921 gattcatcct taccggagtg ccccatgcca tgaatatcaa ccgcattatt tttgccattg
    40981 aagactgtat cagtaccctg tcacggtatt ccgtttccgc attcttcccc gattactgtg
    41041 atctgcacac cgtcatcggt ctggaccagc aggaccgcga acgccgcccc tggctgaaag
    41101 ccccctacat tgccaccaca aaacaggggt attacctttc cgtctttgaa atcagcggtg
    41161 ccctgaaaga aatggatgaa aacccggacc agacggcccc cgggacactg gagtcactga
    41221 ttgcagcact ggctgagcga atgaacacgg cctggaaaaa ttccggccac aaaatcagtt
    41281 ttgttttcga acgcgatccg gagcgtggaa gggatgaaat cgaagccatg ctcttacccc
    41341 agcgaaaatc catcgcacgc acggggatcc tgcttcagga cgtcctggat gaaaaagtca
    41401 ccacactcac cccctggctt gtccgggagc gctgctggct gaccgtctgg agctctgaag
    41461 aactgctgag ccggaccgat cgcaagaacc atcagacccg tgtcaggaaa ctcgctgaac
    41521 acgctccgcc agcccgtttt gcgcaggatc cctggcgctg gacactttcc gccctcaaaa
    41581 tacggcatga cgccttactg gacacgctgg agcaggctct gacacatgat tctgatggcc
    41641 tgctgattcg cctgatggat atccatgaag tgggacggga aatccgccgc caactggagc
    41701 gcaacagtac accggcagta tggcagcctc atctgcccga agatgcccgt cctgccggct
    41761 ggcgacaggg cggggatacc tctgtattcc acgcaccgtc gctgaatctg cagttattca
    41821 ccacacagcc tgaaacacac ggcagcctga ttcaggccgg tgagctctgg cacggtatgg
    41881 tcgccattac cctgccccct cagaatctgc ggacattcaa ccagctggtc agggatgtgc
    41941 cccgtgcaat cccctggcgt atccggatgg atctgatgcc cgacggcatg aaggcgctgg
    42001 gggtcaagaa aaccctgctg acctacagca gctttattcc tcctctccgg cccatgtatg
    42061 actcggtaat gatgctgaat gacatcaaca agcatgatcc ggtctgtatc atgaccattg
    42121 ttgccaccac atggggaaac agccgggaag tctgtacccg taaccaggct ctcctgaaat
    42181 ctgcgctgga aggctggggg gtatgcggca ccaccacaac cttcggtgat ccgagacgtg
    42241 cctgggtgaa cacggttctg gcagcctccc acagctccgg cccgatcccc ctttacccgc
    42301 ccctttcaca cgccctttcc ctgtttcccc tgaacagggc aggctctgtc tggcggggac
    42361 agggaaatct gatgatgcac accgaagacg gctctgcatg ggaggtggcg ctggcgtcat
    42421 cacaacagaa caagcacacg gagctgacac ccggcgcacc cggtctgggc aaatcggtac
    42481 tcatcaacgc actgtcggag atccagatag cctcagccca gaagaatctc cctttcattg
    42541 cctatatcga caagggattc agtgcacagg gactggtaca gcttatccgt gacagcctgc
    42601 ctgagcagag aaaggacgaa gccgtgggga tcattctgag taatgacccg gaccacaccc
    42661 gcaacctgtt tgatgtgatg tacggggcca gaaaacccgt cacaccggaa aagaacttca
    42721 tggtctccgt cctctgtgca ttgtgtgtgg ataccggcac cggtcagccc tgtaatcccg
    42781 gcgacacccg gcaaatcatc agcagcctgg tggacctggc cttccgggaa tatggcgaga
    42841 ataaccccag actttaccgc gcaggtacgg aacccctggt ggatctcgcc ctggaagaga
    42901 gcggcatcgc tgaacaacat gatgccggat ggtggaatgc cgccacctgg tttgaaatca
    42961 gggacatgct gcatattgca gggaatatcc ccgccgccca gcgggcacac taccaggcca
    43021 tgcccctgct ggctgaaatg tccgccctgc tgggacagcc gtccatccgg gatgtgttcg
    43081 gcaccgttca gcgtgataac tcggaagagc ggttgctgga ctacatccgc cgtgccctcg
    43141 atcagggaca cagtgactac ccgatgatgt ccggctgtac ccggtttatg ctcagccccg
    43201 acacccgcgt ggtcgccgtt gatctgaaca atgtggccgg ggacaagaca cctgccggac
    43261 gtctgcgcac cggtatcatg tatctgctgg ccgggcagat cgccggcggg gattttgtcc
    43321 tgccgcagta tcaggaggaa atacggaaaa atctcgaccc acgctaccat gaggtgattt
    43381 tccgccgcat tgaacagctt gatcaggagg tcaagaccaa agtctatgac gaactgcaca
    43441 atgcaaaagg gatcaacttc atctgggaag ccctggacac ccaggagctg gagcagcgta
    43501 agttcgggat acgcacggtg ctgagcaccc agctgctgca gcattatcct ccggccgtgc
    43561 tcaaatccgc caacaccctg tggctgctgc gttaccgccc tgacgaaatt ccgttcctgc
    43621 gggataactt tggcgtcccg gaagtcacac tgcggcgctt cctgaaaatg ccggaagggg
    43681 ccgcaccgga cggcagcggt gtcccggtac tggctgtgtt ccgtgtaaaa aacggcacgc
    43741 tggcccggat cctgaaattc accctcggtc cactggagct gtgggcactg aactcctctc
    43801 ctaaagacag cgccctgcga cgtgcgctga cgcaggaggt ggggtcactg cgggcacgcc
    43861 agatcctggc cgaacacttt ccccggggtt cagccacctc actgatagaa caccgcgccc
    43921 gcacacatga cagtgaaaac gtcatccatg agctggcagc agaactcatc cgtaagcagg
    43981 gatataacct ctgatgtaca gcattcatat caccccccgc tgccggcgtc ctgcccgggg
    44041 acaggtcttc ctgaaaacac tgcgcgtgga cggtgacagt ttcagcttca ccctgccgtc
    44101 cgcctgcttc acactgcgac cgtcccccaa tccccgggtc atttttgcca gtgacagacg
    44161 ggctgacggg gagatgagtg gtattcgcct ggccctgccc ggagagacag gcacgctgat
    44221 aacaacggcc acctggcagg ttaacgtgcc ggagcgaccg ccttttgata tacagcatgt
    44281 cattcactgg cttctgcccg caccactcac tgcgctgatg tctgacaaca cctggctgtg
    44341 gccggatgtc cccggcacac cggcactcac agccggagac tggccggtca tggaaagcga
    44401 caccccgaca ctgaaacgcg tgaagcgtcg taccttcacg ctcatcgata accgtaaccg
    44461 tcagggatgg atcacgcgcc gcttccagtg tttcccgctg gctccgctgc cgaaatcact
    44521 cagtcatcgc gtaccagaca tcctgtatcc cccgaaaacg gagaaaaaag aatgcaaaga
    44581 caatctgcaa ccacactgct tgcactcctt gtaggctgtg cggttatccc tgccacccat
    44641 gcctactctg tgaatgttgt gtccagtcag ccggtcaccc gacagatcat gccggagctg
    44701 gccaccatac agggcatcat ggaggaaatg gtccgtatgc agacggccac cggcacagcc
    44761 atcacccaga actcagaaaa actggccacc gtcattgccc aggacgggca ggccacccgt
    44821 cagcagatga tattcagtaa tgaaacacac cgcctcgagg aggccaggaa aagcttcagc
    44881 gtgccggact ccatctgcag tgaatccgcg tccggcattg cagcagaaag ccgccgggca
    44941 gcagcgtctg cagcggcacg tctgagtcag gggggagggg tcagcagtaa acccatccgg
    45001 gaacgtctct cccgggctgc ggactccccg gtccgcgagg cgtatgatag tgccggcatc
    45061 cacgccggtt actgcacgga agcggagtat gcccgctttg gcggaactga tgtctgccct
    45121 gccgtgggcg atctgccggg cggagacagc caggtacgat cgctctatca gggcgcaggc
    45181 acagcagata ccccggccgc tctcacctgg gatcagaaac agattgatgc cgcgacggct
    45241 tatatgaaaa acaccgccag accgtcagcg ggtcgtgccc cggggaaagg agaagtgggc
    45301 acacagaccg ggcgcacgta tgtgggcctg cagaatgaat acaacggcat catcgatgcc
    45361 gcctctcatc cgcagctgtc actgattgct gacagtaccc ctaatgaagc cacccgtggg
    45421 gcactgactg aagccctgca gtcaccgtca gctgcggcat attttgaccg gaccgcatca
    45481 tcagaagccc gcacccgtgg ccacatgagc cagcgggagt ttgaggcctt tgaagccggc
    45541 agacggtatg ccaacaccga ctggcagcag gatctgcagg gcatggaagg cgataacctt
    45601 ctccgtgagc tgctgcgcac caccgccctg ctgaactggc agatgaacga tctgaaagag
    45661 cagattcgtc agggtaacgt tatcgccggg cagcaacttg cgctggccgc acggcagtat
    45721 tacggacagc gccttggcga actgtcacag gccatgagcc aggggagcgt aagatgaaag
    45781 acgacaacag catcgacgaa aaaaaaccgg ccccccgtct ccgccgcatc ggtgatatag
    45841 ggataaatct gttcgcacca ctggccgaaa cccgctggat gctgcgcagc tgccgccata
    45901 tgctggacca gcataaaacg cgcctgaagc gcctcatgcc cgaccggcaa ccggagagtc
    45961 cggtctgtat gacctgggag gaagcggtca gggccagcgg actgacggtc gacgaactgg
    46021 accggcgtct gcgacgtcgc aggatgttct ggcggacagg attctggctg atgatgatac
    46081 cggtacttct gctgtgtggt atggcccttg cggccaccgc actgccggtc atcaccctga
    46141 tacgtatcct ggtctgcctg ttactgatga gtaccattgc agcactgtgt gcctcaaaag
    46201 ccctgattgc gtcataccgc ctctggcagt tgaatgaacg tcgggtcagc gaggacgaac
    46261 agggcacatt ccccgattac ctgcaggccc ggcacggctg gaaacaggcc acatggctgg
    46321 ccattaccca ccggcagtac tgactgtccg gtctgcaatt tcttttcctc attccggggg
    46381 ctgatgcccg ggcatcccct tcggttccgg agttaccgtg aaaatcatac ccagagcact
    46441 gagtgcaggt ctggcgctgg ctgccgtacc tgcacaggct ttcgtcacct atcaggacat
    46501 tgtgagtgcc gccaccaatc cggatgacct gtcgcgccag gcactggtca ccatttttgg
    46561 tgatgtggtc acaaatcccc tgacagccag cagcaacacc atcattggca acctgttcat
    46621 ggtgtttaac gcaattatcg ccacgatggg gatagtctgg tttctgttta ttggtatccg
    46681 tcatgctgtc cgcacgggac accagggcag tgtctttaac aacggacggg acgtcgtcgg
    46741 catactcagt gtcgtgagtg gatttttgat gattgtgccc accgcttccg gctggagtct
    46801 ggcgcaactg gtcatgctgt ggggcgcatc catcatgggc atcggttccg ccaatatcat
    46861 ggttcagaca gcggcagaaa acattgctga aggctattca atgaccgtac agccagttca
    46921 tgtctccacc cgcacggcag cacggggaat tctggagatg gaactgtgca aacacgccat
    46981 gaatacgggc ctcaatgact ttaaccgtac cgcacgttca tccacgccgc ccatgagcga
    47041 atcctcacgg acagagaatg gcagatatac cgtcaccgtc tcaaatggca gcgccacctg
    47101 cgggagtgtg agtctctccg tgaaaggaaa cggcagtaac aagcagtccg cgctggccca
    47161 ttttttcaat cccttcattg aaacggaata tcagggggtt ctgacggcac agcgctccgc
    47221 catggatacc atgctccgtg acctggacag cgcggcgcag gaatttgtgt cggcataccg
    47281 gacccggcgc gacacgggta acggcaacct gccggatatt gaaagccgta ttcagaaggc
    47341 agccaaccgg tatgaagaaa cggtgcagaa ggcgttgccg gcagataacg cagaaactga
    47401 gcgcagggag cagctgaaat cctacctgac cacctacggc tgggttgccc tgggtgcctg
    47461 gtaccagacc tttgccaccg ccaaccagcg actggaagac ctggccaacc gcgcccctac
    47521 cgtgagtggc gtctcgtcac tgggcgaagc cggggatacg gaattcctga aggaagtcaa
    47581 gggggcttac caggcacagt tgcagaattc accatatact cctgcactgg gaacaaacac
    47641 ttcatcaact gaatacagaa cagcggaagc cgctgaacct aaaagcataa taatgaaagt
    47701 atggtcgggt gaaaattttg cagacaaaat tgcgacatcc ttatctggaa caggaacatc
    47761 ctcggaccaa ctaaatccac tgataaaaat gaaaaatatc ggtgattaca ctatgggtat
    47821 tactgaaagc atatgggcgg gttataccac cgtcagggta ctttccacag ccggcagcaa
    47881 ctccgttttt ggtcgactgg tcaatgccgt cagcggatta tctgacgccg caaaggcttt
    47941 tctggaagca tccgcaccac cactctactt cctgctgttt ttgctgttct gtgccggctt
    48001 ttcgttatcc atttacctgc cgtttatccc gtttatcttc tggatgaccg gcatcggcaa
    48061 ctggatagtc agcgtactga ttggatgtgc tgctggcccc ctgtgggccg ccacccatct
    48121 gggaacaagc caggacaggg gaagccgggc tgcttacggt tacatctacc tgatagacag
    48181 catgatcaga ccgctgctga tggtgttcgg attctttttt gcatcggtcg ccatcgtggc
    48241 cggcggcacc attctgaatg cgttgtttgg cgcggctctg gttaatgtgc aggcggactc
    48301 ccttacgggg ttattcagca tcgctggatt tttgctgatt tatgcccgag tctgtaccac
    48361 actggttgca gccgtttttg cgcttcaggc ttatctgcct gaccatgtaa ttaattttct
    48421 gggcggacgt gacggtgtta atatgctggg gaatatggtt tcttcagtca aagatattat
    48481 tgccggaggt aataccaata tgcgcagaac acctggcgtt cgtgaagaaa aactgaaaaa
    48541 tgttaatgga gatgataaag acggaataaa acaataaata aaaatatcga aaaaccaata
    48601 aaacactatt gccatattca cctgaagaga ttaaaatata ttcaggtgaa tatgggggtt
    48661 tattatgagt acaacaaaaa gcagatatcc ttcctggtgg ccatattatt accttgttcg
    48721 caatgcgtat ttcatatttg gcataccatg cttattattt tatgcttttt tcggaacact
    48781 aattgttcat gattcagcct tatctcaaac aatggatttt atttttgcat atggatgctg
    48841 ggcattaata attctcccat tactctggct atatctgaga accagagcga aaaaaaagaa
    48901 agtccagcag gttgttcagc agataaaaga aagctgtcac tttgctccaa ccagcgatta
    48961 tgagcaactg tcattctcga agagttgtta tttcgggatc gacattaaaa acggcaccat
    49021 gctgtatgtg cgcatttatc cgaacaatgt gatggatgtg attggtctgg atattcacaa
    49081 ctttacccgc acggttgctg aagacggcaa gctggaaatc cacactacat atgtgagtct
    49141 gccgatgatc ccactggaaa tctccggcat ctcatcaagg acgctggcca acaccatgca
    49201 caccatggct gcacgtggat atgagtacaa ggaacgtttc ccacagatga tccggtatcg
    49261 ggttaaagag tgggaaaaag ttgccggtgt tcctgttgca gaagtatttt gaggtagcta
    49321 tcaactgtta gacgataatc cgtcagccgg ttttctctga accggctttt tcttttcagg
    49381 cagaatgcct gtctgccgtc tatctcagga cgccgcatgg agtctctatg ttttccccac
    49441 aaattcttag cactgtcttg cccactgtag acaaaatcaa tacaattctg catatcagtt
    49501 atctataaaa gataacaata tcattataaa taattatcat aaccacacac tcaaggcagt
    49561 acctagaaaa cataaagata aaatcatgtc tgaatcccgc agcaattttc cagcacgaca
    49621 tggtactcca tggaatactg aagagttaca gcgtcttctc gtatccatag cggagggcgt
    49681 ctcgctaaaa aaacttactc aaacacatca acgatcgtcc gggggaatac gtggagcaat
    49741 caaacgcctg cttccttctg agtttcttga gttcaacgat ataaacacgg tgaatgatct
    49801 cagcaattac ttcaacaaga ttcctcgcca agaacacaat agtatcatca ataatttaat
    49861 atcattgagc actgcaaaaa caattgctca tgataaagtt gaaatacaaa agaatagttt
    49921 taaacaaaat accccactcc ccctctgcca acccaataca gattttaaag attatatagc
    49981 cgatgaacat gatgtcatta tgctaattca ttctgctatc gaaagtttat ctaataaacg
    50041 acatcaatat attcttctaa tgcgactcgg cacagatgat tcacctcatt ctctggcaga
    50101 tattgccgtc aaattaggaa tttcccgtga aagggtaaga caacttcagg aacgagccct
    50161 tggcttacta gctgctaaat ctcgctatga agggacacct ggagcttgcc ttaaagccct
    50221 tattaactca ataggtaatt caccatatga tatagcagta tggatttatc gtattgttca
    50281 tcgggatttc gttacatcct ctgagttagc cgccaaattc atcatttttg ccgcaggcat
    50341 tcctaaagca agacgagatg aaataaaaaa ttcgctctca ttaatatcac aattacaaaa
    50401 gcagaagtta cgtgaacgaa gagcagaatt agcacacgct cacgctcagg aaagagcccg
    50461 aatcaagaaa gaaagagttg agttcatatt cagtaaatgg ctagacaata cacattggcc
    50521 aaaatatatc gcacctcctc cccccgtaga acagctatgt tcacagagag cggttaatga
    50581 tactgacata tctggattct tttactctca aaaattaggt cgtacagtcc agtacgaatc
    50641 agggcttgag tttaaaataa tgaatctatt agaatgttgc gatcaggttc ttttctatca
    50701 agaacaacca ttttctattc catattgttt taaaaacaaa tcaaaaaaat actatccaga
    50761 tttactcatt gcgacagttg atggtcggtg cctacttatg gaagtgaagc caacagaccg
    50821 aatgaccttg agtctcacga gaataaaaag taatgcaggt cgcaaatggg cacatactcg
    50881 aggatgggga tggttggtca ttagtgatag gttctcgtta cgtcagctgg aagaacatgc
    50941 catctcaaca aacacatgga aacttataga ggctgaactg aaaacatcaa ggattttagc
    51001 atggcgtgaa atgatggagt tacgtacacg atacgagatt catcgtttag atcttattgc
    51061 atatatcatt cagtctggtg ctgaagtgga taacttttat cgaattaccc cgaaaatatc
    51121 taataataca tctaaccaga gacaaccaaa tgagagcgac tgtcataatt gatgacgcac
    51181 aggacggaaa aaataatagc tgccggacag cagcatccag cagtcgtccg gtaaaaaaga
    51241 caaacaaggc gcattgaggt cagccttcgc aacaatccgg cggcagatgt cctgccgccg
    51301 tgttgcccgt taacgtttaa cttcgtaggc taacgttgcc acaaacaccg tatttccctg
    51361 ctcgatacga aatccgcaaa gcgaatccct caccatccag acaaaacaca gtatcgtcac
    51421 acagacgacg attaacatca ttaaaaacgt tcgttgtggc atcgcagccc ctccattgcc
    51481 tttcggccgt catcgggcta ctataatgtt gtctaggcat tacagtgggc cctgggttga
    51541 ttgataaaaa aatcgcctgg ggctttctgc tacatgccgt ccacaaaacg ctttcggcat
    51601 acagcctcaa gcgcccgccg ctattctaat cgttttcctg tccgccacaa tccttttcat
    51661 gccaccacgt attttgtgtc agggacagaa ataaagccgg aaagtgacta cgtgtgcaca
    51721 catctgtttt ccgggctgca ggcatcgtgt tattttctga aatgcaaaat aataaaaaac
    51781 aaggacacat catgaaggat gaaatacaca gtaccagcat taacccggat gttgttgagc
    51841 agtttctggc ccttatgaat acacattgta aatcctcaga agaggcgctc accatgctga
    51901 gtacgctttg ttacaacctg atgatgacat gcggtatcca ttccatggac agccagatgc
    51961 tcaatgggca tgggatatcc atgcaacttc aggggattga tattgccgaa aagccaggca
    52021 agaatattca ctaacctgaa cagggcctga aaacgctgtc ttttcaggcc tctgcaggca
    52081 aaaactgatg acaacaaaat ttctgcgttt gtaccccacc gatgaaaaag acaaaaactc
    52141 acaccgggct tctcatcatt aaagataaaa cccgacgtgt cagcctctac gaaacgccaa
    52201 cggcatggtg tatcaggggg caggagtgct acagcaaaag cactggacgc cgctgtggca
    52261 gccacgacag tctgagcaga ctgcgcctgg acagcattaa gcctgttgaa tgagtggtgg
    52321 caccatgaaa gataacaaca caaccgccac cccagaaacc tggaaaatgc gttttctggc
    52381 caacgggaca tccggcacat ggttttacac tgacgacatt gcagtcactg acgcgtggct
    52441 tcgcgcgcac ccggatgatg tggaggttat ccctctctgc cggatacagc cccgccagca
    52501 ttcccatgaa caacatattg aagaactccg cgcagccctg gcccggatgc tccatatgca
    52561 tcatatgatg ctggaaacca cacattaccg ggaaaatatc accgatgccc gttgtatccg
    52621 tgaaatgcat gaagcgccat tacaggcgct caatattctg tccagatacc cgcataaaaa
    52681 ggacgaggtg tgacgcatgt ggctactgat tttctggctt tcatcgccct ttaatgtctc
    52741 ttccggtgat gcccgtcagc ccacactggc ggtgcacagc caggcctttc ataccgaacg
    52801 ggcctgccgc gatgcattgc gcgccattaa aaaggagaat gttggcgaat tttctctgcg
    52861 gggcgtttgt gtttctgacg gaaatacgaa ggaggaggct gagagctcat gacggatatt
    52921 actgacagac atacacttcg ccgtatgtcc tggtcggaac tcatcacggc aggacaggaa
    52981 gcagaatttc agcatgatta cgagcgtgcc agaatagtgt ggtcattcgc cttacatgtt
    53041 gcaacaacca cgattaacaa aaatctggcc atagctcata ttcgacgctg cgatactttg
    53101 ttgcataaat caaaggctgt acccggaaac aacccgtgtg gttccggtca gcgagtctga
    53161 gacctcaaca tcccgggtga tgattgtcat tccggactga cgcagccgct gcacatctgc
    53221 ggcaatacgt tgaataccaa gccagaacag accatccagc gcggtgacaa ttaacccgct
    53281 ttccagcgcc agtttcagcc gtgctgtcgg ggctttaacc tgcccggcta tatcagtgaa
    53341 acaacgcgtc gtcatgccat agctgtccgc ccgccgtatt cgcgtcgcaa gactgtaact
    53401 gaagctatcc ggcacgccgg ataagtcatt ctgcagactg tatacacatc cccgctgccg
    53461 ctcattgaat acaacttttt tctggctgag ctgccattcc cgccgcacgc tgtcaatcat
    53521 ctgcggtgtc cgggttaaca gcagacgaaa cggcagttca aaactggtca cataatcaac
    53581 attaagtaag gcaatccgca gccgctccct ggctcctgct ccggtatcac ggcattcacg
    53641 catgacatct tcccactggc gcatcagtcg cggtgactca ttcctggccc tgaactgata
    53701 tttctcaatc tgataatcaa tatgttcctg cataccgtca ccggatatct gttgccgtat
    53761 catcacggat atcagcgtac tggaattcat gccctgcgtg aagtatcaca ctgccaaaaa
    53821 aaacacacct gaacgttatt atttcgcaat attaattgca cctgttttca taaataaaaa
    53881 ggagacgatg aaatgataaa atatatattt tcactcacca ttattttttc cgcctctctc
    53941 tgtgccgcac agattcaggg gcacgtcatc cgcgttcttg atggtgatac gctggaaata
    54001 ttacaggaca aaaagcctgt cagggtccgt ctggctaata ttgatgcacc ggaaaagaaa
    54061 cagccctttg gacgctggtc agccactcag ctgaaggacc tggtggccgg aaagcccgta
    54121 accgtgcaat acgcgcagac agacaggtat ggccgtattc tcggccgggt ctataccgct
    54181 gacggcacag aagcaaatcg ctggcaggtc cgtaacggcg ctgcctgggt gtacccggac
    54241 tacaataatg actatactct cccgccgctc cagcgtgatg cccgcgccct gaagcgggga
    54301 ttatgggcag ataaaaatcc ggtaccacca tggacctgga gaaaacagaa taagaaataa
    54361 ttatttgctg atatgaagaa aaaaaccatt tcaaagaaaa taaatggcaa aaatacagca
    54421 tggaaaaaat tgccattttt accgccgcgt tatttttaat atttgctgtc tgctggtcgt
    54481 aataagcaga ataacagaaa ggtgtaaaaa tgaacctggt tgatgcattt gtcactaaag
    54541 tgatatccgg accgtatgaa gaatacggaa aatggtgggt ggatgtagaa tatatttcct
    54601 ggggagttcc gggtaaaagc cggctcatgt ttgactcacg ggaacaggca caggaagtgc
    54661 aggaaggttt tcgattttta acctgagcat attttgttaa agggcaga
//
LOCUS       DAZFRE010000023        53144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFRE010000023 DAZFRE010000000
VERSION     DAZFRE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.023"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            251..403
                     /gene="mokC"
                     /locus_tag="ACHKUI_001627"
     CDS             251..403
                     /gene="mokC"
                     /locus_tag="ACHKUI_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6597845.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(508..1638)
                     /gene="dnaJ"
                     /locus_tag="ACHKUI_001628"
     CDS             complement(508..1638)
                     /gene="dnaJ"
                     /locus_tag="ACHKUI_001628"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6597846.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1727..3643)
                     /gene="dnaK"
                     /locus_tag="ACHKUI_001629"
     CDS             complement(1727..3643)
                     /gene="dnaK"
                     /locus_tag="ACHKUI_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6597847.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4015..4419
                     /gene="yaaI"
                     /locus_tag="ACHKUI_001630"
     CDS             4015..4419
                     /gene="yaaI"
                     /locus_tag="ACHKUI_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6597848.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEEQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4445..5158
                     /gene="msyB"
                     /locus_tag="ACHKUI_001631"
     CDS             4445..5158
                     /gene="msyB"
                     /locus_tag="ACHKUI_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6597849.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGEFQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5307..5873
                     /gene="satP"
                     /locus_tag="ACHKUI_001632"
     CDS             5307..5873
                     /gene="satP"
                     /locus_tag="ACHKUI_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6597850.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            5932..6030
                     /locus_tag="ACHKUI_001633"
                     /pseudo
     CDS             5932..6030
                     /locus_tag="ACHKUI_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6078..6665)
                     /gene="mog"
                     /locus_tag="ACHKUI_001634"
     CDS             complement(6078..6665)
                     /gene="mog"
                     /locus_tag="ACHKUI_001634"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6597851.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6780..7733)
                     /gene="tal"
                     /locus_tag="ACHKUI_001635"
     CDS             complement(6780..7733)
                     /gene="tal"
                     /locus_tag="ACHKUI_001635"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6597852.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGDIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8012..9442
                     /gene="yaaJ"
                     /locus_tag="ACHKUI_001636"
     CDS             8012..9442
                     /gene="yaaJ"
                     /locus_tag="ACHKUI_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6597853.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9512..10288
                     /gene="yaaA"
                     /locus_tag="ACHKUI_001637"
     CDS             9512..10288
                     /gene="yaaA"
                     /locus_tag="ACHKUI_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6597854.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNAQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10441..10737)
                     /gene="yaaX"
                     /locus_tag="ACHKUI_001638"
     CDS             complement(10441..10737)
                     /gene="yaaX"
                     /locus_tag="ACHKUI_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6597855.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10951..12237)
                     /gene="thrC"
                     /locus_tag="ACHKUI_001639"
     CDS             complement(10951..12237)
                     /gene="thrC"
                     /locus_tag="ACHKUI_001639"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6597856.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEISQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12238..13170)
                     /gene="thrB"
                     /locus_tag="ACHKUI_001640"
     CDS             complement(12238..13170)
                     /gene="thrB"
                     /locus_tag="ACHKUI_001640"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6597857.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(13172..15634)
                     /gene="thrA"
                     /locus_tag="ACHKUI_001641"
     CDS             complement(13172..15634)
                     /gene="thrA"
                     /locus_tag="ACHKUI_001641"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6597858.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15660..15779)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15715..15780)
                     /gene="thrL"
                     /locus_tag="ACHKUI_001642"
     CDS             complement(15715..15780)
                     /gene="thrL"
                     /locus_tag="ACHKUI_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6597859.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15994..16680)
                     /gene="yjtD"
                     /locus_tag="ACHKUI_001643"
     CDS             complement(15994..16680)
                     /gene="yjtD"
                     /locus_tag="ACHKUI_001643"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6597860.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17080..17220)
                     /gene="yjjY"
                     /locus_tag="ACHKUI_001644"
     CDS             complement(17080..17220)
                     /gene="yjjY"
                     /locus_tag="ACHKUI_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6597861.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            17316..18032
                     /gene="arcA"
                     /locus_tag="ACHKUI_001645"
     CDS             17316..18032
                     /gene="arcA"
                     /locus_tag="ACHKUI_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6597862.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18091..19443)
                     /gene="creD"
                     /locus_tag="ACHKUI_001646"
     CDS             complement(18091..19443)
                     /gene="creD"
                     /locus_tag="ACHKUI_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6597863.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19501..20925)
                     /gene="creC"
                     /locus_tag="ACHKUI_001647"
     CDS             complement(19501..20925)
                     /gene="creC"
                     /locus_tag="ACHKUI_001647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6597864.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20925..21614)
                     /gene="creB"
                     /locus_tag="ACHKUI_001648"
     CDS             complement(20925..21614)
                     /gene="creB"
                     /locus_tag="ACHKUI_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6597865.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21627..22100)
                     /gene="creA"
                     /locus_tag="ACHKUI_001649"
     CDS             complement(21627..22100)
                     /gene="creA"
                     /locus_tag="ACHKUI_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6597866.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22311..23180
                     /gene="robA"
                     /locus_tag="ACHKUI_001650"
     CDS             22311..23180
                     /gene="robA"
                     /locus_tag="ACHKUI_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6597867.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23177..23824)
                     /gene="gpmB"
                     /locus_tag="ACHKUI_001651"
     CDS             complement(23177..23824)
                     /gene="gpmB"
                     /locus_tag="ACHKUI_001651"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6597868.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23876..24403
                     /gene="yjjX"
                     /locus_tag="ACHKUI_001652"
     CDS             23876..24403
                     /gene="yjjX"
                     /locus_tag="ACHKUI_001652"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6597869.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEVLGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(24476..24802)
                     /gene="trpR"
                     /locus_tag="ACHKUI_001653"
     CDS             complement(24476..24802)
                     /gene="trpR"
                     /locus_tag="ACHKUI_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6597870.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(24892..26829)
                     /gene="sltY"
                     /locus_tag="ACHKUI_001654"
     CDS             complement(24892..26829)
                     /gene="sltY"
                     /locus_tag="ACHKUI_001654"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6597871.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27036..28703
                     /gene="ettA"
                     /locus_tag="ACHKUI_001655"
     CDS             27036..28703
                     /gene="ettA"
                     /locus_tag="ACHKUI_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6597872.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28759..29043)
                     /gene="nadS"
                     /locus_tag="ACHKUI_001656"
     CDS             complement(28759..29043)
                     /gene="nadS"
                     /locus_tag="ACHKUI_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6597873.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(29045..29377)
                     /locus_tag="ACHKUI_001657"
     CDS             complement(29045..29377)
                     /locus_tag="ACHKUI_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6597874.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPINAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(29469..30701)
                     /gene="nadR"
                     /locus_tag="ACHKUI_001658"
     CDS             complement(29469..30701)
                     /gene="nadR"
                     /locus_tag="ACHKUI_001658"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6597875.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLIDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30722..32104)
                     /gene="radA"
                     /locus_tag="ACHKUI_001659"
     CDS             complement(30722..32104)
                     /gene="radA"
                     /locus_tag="ACHKUI_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6597876.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSVDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(32153..33121)
                     /gene="serB"
                     /locus_tag="ACHKUI_001660"
     CDS             complement(32153..33121)
                     /gene="serB"
                     /locus_tag="ACHKUI_001660"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6597877.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            33227..33871
                     /gene="ytjB"
                     /locus_tag="ACHKUI_001661"
     CDS             33227..33871
                     /gene="ytjB"
                     /locus_tag="ACHKUI_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO6597878.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33899..34915
                     /gene="lplA"
                     /locus_tag="ACHKUI_001662"
     CDS             33899..34915
                     /gene="lplA"
                     /locus_tag="ACHKUI_001662"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6597879.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            34947..35210
                     /locus_tag="ACHKUI_001663"
     CDS             34947..35210
                     /locus_tag="ACHKUI_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6597880.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(35371..36090)
                     /gene="deoD"
                     /locus_tag="ACHKUI_001664"
     CDS             complement(35371..36090)
                     /gene="deoD"
                     /locus_tag="ACHKUI_001664"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6597881.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(36147..37370)
                     /gene="deoB"
                     /locus_tag="ACHKUI_001665"
     CDS             complement(36147..37370)
                     /gene="deoB"
                     /locus_tag="ACHKUI_001665"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6597882.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37422..38744)
                     /gene="deoA"
                     /locus_tag="ACHKUI_001666"
     CDS             complement(37422..38744)
                     /gene="deoA"
                     /locus_tag="ACHKUI_001666"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6597883.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISGGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38822..39601)
                     /gene="deoC"
                     /locus_tag="ACHKUI_001667"
     CDS             complement(38822..39601)
                     /gene="deoC"
                     /locus_tag="ACHKUI_001667"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6597884.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39859..41409
                     /gene="yjjI"
                     /locus_tag="ACHKUI_001668"
     CDS             39859..41409
                     /gene="yjjI"
                     /locus_tag="ACHKUI_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO6597885.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IVARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41381..42244
                     /gene="yjjW"
                     /locus_tag="ACHKUI_001669"
     CDS             41381..42244
                     /gene="yjjW"
                     /locus_tag="ACHKUI_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6597886.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(42461..43240)
                     /gene="yjjV"
                     /locus_tag="ACHKUI_001670"
     CDS             complement(42461..43240)
                     /gene="yjjV"
                     /locus_tag="ACHKUI_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6597887.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQQGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(43237..44310)
                     /gene="yjjU"
                     /locus_tag="ACHKUI_001671"
     CDS             complement(43237..44310)
                     /gene="yjjU"
                     /locus_tag="ACHKUI_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6597888.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44432..44593)
                     /gene="ytjA"
                     /locus_tag="ACHKUI_001672"
     CDS             complement(44432..44593)
                     /gene="ytjA"
                     /locus_tag="ACHKUI_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6597889.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44720..45325)
                     /gene="osmY"
                     /locus_tag="ACHKUI_001673"
     CDS             complement(44720..45325)
                     /gene="osmY"
                     /locus_tag="ACHKUI_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6597890.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45718..47307)
                     /gene="prfC"
                     /locus_tag="ACHKUI_001674"
     CDS             complement(45718..47307)
                     /gene="prfC"
                     /locus_tag="ACHKUI_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6597891.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(47398..48075)
                     /gene="yjjG"
                     /locus_tag="ACHKUI_001675"
     CDS             complement(47398..48075)
                     /gene="yjjG"
                     /locus_tag="ACHKUI_001675"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6597892.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(48090..48536)
                     /gene="rimI"
                     /locus_tag="ACHKUI_001676"
     CDS             complement(48090..48536)
                     /gene="rimI"
                     /locus_tag="ACHKUI_001676"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6597893.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(48505..48918)
                     /gene="holD"
                     /locus_tag="ACHKUI_001677"
     CDS             complement(48505..48918)
                     /gene="holD"
                     /locus_tag="ACHKUI_001677"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6597894.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            49021..50052
                     /gene="rsmC"
                     /locus_tag="ACHKUI_001678"
     CDS             49021..50052
                     /gene="rsmC"
                     /locus_tag="ACHKUI_001678"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6597895.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPYTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            50252..50338
                     /locus_tag="ACHKUI_001679"
     tRNA            50252..50338
                     /locus_tag="ACHKUI_001679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50286..50288,aa:Leu,seq:cag)
     gene            complement(50377..50613)
                     /gene="yjjZ"
                     /locus_tag="ACHKUI_001680"
     CDS             complement(50377..50613)
                     /gene="yjjZ"
                     /locus_tag="ACHKUI_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6597896.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            50754..51542
                     /gene="fhuF"
                     /locus_tag="ACHKUI_001681"
     CDS             50754..51542
                     /gene="fhuF"
                     /locus_tag="ACHKUI_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6597897.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(51580..52257)
                     /gene="bglJ"
                     /locus_tag="ACHKUI_001682"
     CDS             complement(51580..52257)
                     /gene="bglJ"
                     /locus_tag="ACHKUI_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6597898.1"
                     /translation="MEHSRIKKRNVALIEKCVMSRIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(52215..52940)
                     /gene="yjjQ"
                     /locus_tag="ACHKUI_001683"
     CDS             complement(52215..52940)
                     /gene="yjjQ"
                     /locus_tag="ACHKUI_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6597899.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        12619 a        14318 c        13231 g        12976 t
ORIGIN      
        1 aaattgatgt attgaaatat tattccgcaa aaatgcgagg cgtcttcaga aaaagaattg
       61 ataaaacaga agtgaattaa aagcgcagta taatagcggc gggtgcttga ggctgtctgt
      121 ctcaggcatt ggctgaacgg cagatagaga aaagccccga gtgatatttt accatcaacc
      181 cgaggccccc tatatgcaga acacatgtag agtgcctctt actgaccgaa aggtcaagga
      241 gaagagagca atgaagcagc ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac
      301 cgccgtagtg gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg
      361 ccagacggag gttgctgttt tcacggctta cgaatccgag taagagcaac ggcggggagt
      421 gatccccgcc actttacagg tgctcgcata tcttcaacgc accctggttt ttaccctggc
      481 ctgcccacgg gcaggctttt ggggaggtta gcgagtcagg tcgtcaaaaa acttcttcac
      541 gccatcaaag aagctctttg aacgcgggct gttgtgctcg ccggttgggc caccaaagct
      601 ttcttgcagt tcttgcagca gctgtttctg cttctcgttc aaacctaccg gtgtttcgac
      661 aacaacgcgg catagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
      721 gcgcatacgg aacagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
      781 atcaagggtc ggtacttcga tttcaccacc cagcgccgcc atagcgaagt tgatcgggac
      841 ttcgcaatac aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
      901 aacgtacaga tcgcctgccg gtgcgccgtg ttcacccgct tcaccttcgc ccgcaagacg
      961 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
     1021 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
     1081 acagtgtgga caggtctgct gcacggcaaa gaaaccctgg cgcatctgca cctggccaga
     1141 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
     1201 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
     1261 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
     1321 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
     1381 gccgccaaaa ccgccgccgc ccatgccgcc ttgctcaaac gcagcatgac catactgatc
     1441 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
     1501 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
     1561 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
     1621 ataatcttgc ttagccatct tttttgattt gcccctagat gaatgcacgg gcggagagga
     1681 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
     1741 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
     1801 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
     1861 aaacctgtgc cagttcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
     1921 cagtttccag tgcagtcagc gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
     1981 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
     2041 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
     2101 ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatagtgatc ttctgctctt
     2161 taccgctgtt tttgtctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
     2221 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
     2281 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
     2341 cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt
     2401 ttttcgcgat cagcgtggtc atcacaccgc ccatggtttc gatacccaga gacagcgggg
     2461 taacgtccag cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag
     2521 caccgatggc tacagcttcg tccgggttaa catctttacg cggctcttta ccaaagaatt
     2581 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt
     2641 cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
     2701 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
     2761 gtttcggacc ggtcgcgtct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg
     2821 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
     2881 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccagatag ttgatcagac
     2941 gactgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggttgccaga acttcgaagg
     3001 ttttttcgcc gtcaacttcg tcgatttcga taatggaaat atcgaaagta ccaccaccca
     3061 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
     3121 ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt
     3181 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtaataaca gcttcagtta
     3241 ccggttcacc caggtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaga
     3301 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
     3361 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
     3421 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
     3481 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg
     3541 tgcgatcgcc ttcggcgttc tccagtacac gaggagtggt gccatccata atcgctacac
     3601 aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat
     3661 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
     3721 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
     3781 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
     3841 atggcaggac aagacgtgaa actgacaaca attttattca tataaagaat gaatcgcagc
     3901 caaggcattc atcaaaaaat tgtaataaaa agaaaatatt acgtacctga aacttctctt
     3961 tatcagcagt aaactagttt catcccctta cccctcccca ccaagagaat ccttatgaaa
     4021 tccgttatta cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag
     4081 gcaaacgacc ataaaatcct cggcgtcatt gcaatgccgc gaaacgaaac caacgatctg
     4141 gcgctgaaac ttcctgtttg tcgcattgtt aaacgcatac aactctcagc cgaccatggc
     4201 gatttgcagt taagcggtgc atcgatttat ttcaaagcca cccgaagcgc cagtcagacc
     4261 ctgaatattc cttcagaaat aaaagaagag caaaccactg actggatcaa cattaacagc
     4321 gataacgaca acaaacgctg cgtctcaaaa atcacctttt ccggtcatac agtgaactca
     4381 tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat agaaaccaca
     4441 aaaaatgaat gttaattacc tgaatgattc agatctggat tttctccagc attgtagtga
     4501 ggaacagttg gcaaatttcg cccggctgct cacccataat gaaaaaggca aaactcgcct
     4561 ctccagcgta ctgatgcgca acgaactgtt taaatcgatg gaaggacatc ccgagcgaca
     4621 ccgccgcaac tggcagctga ttgccggaga atttcagcat tttggtggcg atagtatcgc
     4681 caacaaactg cgcggacacg gtaaattgta tcgcgccatt ttgctcgatg tttcaaagcg
     4741 attgaagctg aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga
     4801 acaatttctg cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca
     4861 cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa
     4921 attattggca aaaggtgtgt cgcatttgct ttccagccaa ttgacccgca ttttacgcac
     4981 ccacgcagca atgagcgtgc ttgggcatgg tttgctgcgc ggcgcgggtc tgggaggccc
     5041 tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc agtgcctatc gcgtgacaat
     5101 tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc aggtctgata
     5161 ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg
     5221 ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt
     5281 cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc
     5341 tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt
     5401 ttgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt
     5461 ttgccggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt
     5521 acggttcttt ctggctgacg ctggttgcaa ttctgctgat gccgaaactg gggctgaccg
     5581 atgcgccaaa tgcacagttc cttggtgtct acctgggtct gtggggcata tttacgctgt
     5641 ttatgttctt cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga
     5701 ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taacgccgcg atcatccact
     5761 ttgccggctg gattggtctt atctgcggtg ccagtgcaat ctatctggcg atgggtgaag
     5821 tgctgaacga gcagtttggt cgcaccgttc tgccgattgg tgagtctcac taattattaa
     5881 gctccccccg ctcgaaggga taagattgtt aacaacgtgc gctttgttta tgccggatgc
     5941 ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg caagaagatc
     6001 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt ctgatccccc
     6061 cgcttggggg gattttttta ttcgctaact tcgcgtcttg cactcttcgg tctgaatgct
     6121 gcaaccactt ccggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt
     6181 acgctggcaa aaataccgtg caccacaacg ttaccctcag cgtccttcac accttccagc
     6241 gtctctttaa tagacttcgg ttgaccgggt aagttaagga tcagcgcctg tttgcgaatc
     6301 acccccacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc
     6361 tgttcaccaa agcctggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg
     6421 tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc
     6481 accagctcac acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc
     6541 agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgcagg gatgccttta
     6601 tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta
     6661 ttcatcaaga tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga
     6721 gtgacagaca ggtttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagtatgat
     6781 tacagcagat cgccgatcat tttttccagt ttttcctggt caacagcaaa cttacggata
     6841 ccttccgcca gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac
     6901 tcagtgatac gcgccggacg cgctttcact tcaccagtgt aagacagttt acgttcgata
     6961 tccccttcgc tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcgcag
     7021 cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca
     7081 taaccatgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct
     7141 gccggagcgt actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca
     7201 aacggcgaga tcaggaacac gcccgcttcc gcacaagcac gagcctgagc gaaggagaac
     7261 agcagggtca ggttacagtt gatgccttct ttttccagct gttccgcagc acggataccc
     7321 tgccaggtag aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag
     7381 agtttgatca ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga aagacgcgca
     7441 tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttacg
     7501 gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc
     7561 caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg
     7621 agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca
     7681 gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta
     7741 tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg
     7801 caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct
     7861 gacactgcgt tgatatcgcc cgccattttt atacaaaacc tcatgtatgc tacgcagaag
     7921 ttatcaagta cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa
     7981 gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag
     8041 cgtcctttgg ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt
     8101 tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag
     8161 cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcagc
     8221 gcgcgtgggt agcggcaacc tggccggcgt tgcgctggca attaccgccg gtggacctgg
     8281 agccgtcttc tggatgtggg ttgccgcatt tatcggcatg gcgacctcgt ttgccgaatg
     8341 ttccctcgca cagctttata aagaacgtga tgccaatggg cagtttcgtg gcggaccggc
     8401 atggtatatg gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt
     8461 gctcatcgcc tacgccataa ttttcagcgg agttcaggcg aacgccgttg cgcgagccct
     8521 gagtttttct tttgattttc ccccgctggt gacaggtatt attctcgctg tctttgctat
     8581 gctggcgatc actcgcggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt
     8641 gatggcgata atctgggtac tgacctgcct ggtgatttgc gtaatgaata tcgggcaact
     8701 tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag ctgcaggcgg
     8761 cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc
     8821 caatgaggcg ggaatggggt cgacgccaaa cgcggcagcg gcagctgcgt cctggcctcc
     8881 gcatccggca gcgcaaggaa ttgtccagat gattggcatt tttatcgaca ccctggtcat
     8941 ctgcacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct
     9001 ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct ggggtgcgga
     9061 gtttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta
     9121 tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtctgcg
     9181 gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agtcttccgc tgatgtggca
     9241 actggcagat atcattatgg cctgcatggc tattaccaat ttgaccgcca tattactgct
     9301 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     9361 cccggtgttt gatccgttgc gttatccgga gatcggtcgc cagctttctc cagacgcgtg
     9421 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     9481 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     9541 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     9601 cgcccagcag ctgatccatg aagcgcggaa actgacgccc ccgcagatta gctccctgat
     9661 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     9721 tttcacaccg gagaatgccc gccaggcaat tctggcgttt aaaggtgatg tctacactgg
     9781 cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     9841 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     9901 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     9961 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtag tgataaatct
    10021 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
    10081 gccagtgttc ctcgatgaga agaacggcaa atttaagata atcagcttct acgctaagaa
    10141 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
    10201 gactggtttt aatagcgaag gttacttctt tgatgaagct tcctccagca atggcgaact
    10261 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgtcggatgc ggcgtgaaca
    10321 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattcac gtaggcctga
    10381 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat agttacccgg catttgttat
    10441 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
    10501 gtggcgcggc gatggcggcg gtccatatgg gtgccagcga ttgcctcgcc attcataatg
    10561 ttgtttccac cagccgtggt cgcgccagtg accgccatcc cagtaatagc cacgattatc
    10621 acgatcgcct atctgtaatt ttactgacgg gactagcgta atttccgcag cctgcgctgc
    10681 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
    10741 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacgg
    10801 cagttgttat tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct
    10861 ttttccctgt cgtttttctc catattttct tcataaaaaa gccgggctgc ataaaagcaa
    10921 acccggcctg attgagataa tgaatagatg ttactgatga ttcatcatca atttacgcaa
    10981 cgcagcaaaa tcggcgggca ggttatgcga aagcaagggt aaatcagcac gttctgccag
    11041 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
    11101 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
    11161 acgataagct acggcagcgt gcggctccga ggtatagccc agttctttta actcacgcat
    11221 tgtctgttgc gtggtttcat catccacggc tgcatacccc agctctttca gttgccagat
    11281 tttgcggcgg aacaactctt ccacacgcgg ccagttgttt ggctggctaa catccatcgc
    11341 attggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
    11401 tacggtatcg ttcacgttgg tcgcagcaat aaaacgtttt accggcagac cgagtgactt
    11461 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga ctaccagctg
    11521 gttgcgcgct tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
    11581 caaacggctg atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc
    11641 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
    11701 agtttcgata ttgccgccca atgtacagaa cagtttttct tgcagtggac tgattttgcc
    11761 tcgtggatag aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc
    11821 cgctccagta tcaccggatg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg
    11881 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
    11941 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
    12001 cgccgcacgt acgcgctctt ccaggatttc ctgcgagatt tcatcaccaa taaacgccga
    12061 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
    12121 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
    12181 tacggcttgc gcaaagctga cctgctcatt gtgatctttc agattgtaga gtttcattta
    12241 gttttccagt actcgtgcgc ccgccgtatc cagctggcaa atatgaacaa aaccttcctg
    12301 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
    12361 acacagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc
    12421 cgcaaccgcc tgccgcgcct gccggaagcc aggcaataac cgttcacggt agggttcagc
    12481 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
    12541 gaagcctgcc aaatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
    12601 aatagccctg gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca
    12661 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
    12721 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgcccttc
    12781 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt
    12841 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
    12901 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
    12961 ctcccagcac tgataaacga tattttcccg tggctctgac ggcagcttat cggcaaagcg
    13021 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
    13081 tgcaccatca acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc
    13141 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
    13201 gtagcagatc agcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca
    13261 gtaccaacgg aagcggctga taatagtggc tatagaaggc cagggcgttt tcgccatttt
    13321 tcactttgaa cagcggatca ttaccatcca cttcggcaat cttcacgcgg cagacgccat
    13381 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacac
    13441 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggcg acatcaccct
    13501 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
    13561 gtcccgtttc acgagcgaga atcaatagct tacgcgccac atccatacca gaaagatcat
    13621 ctcgcgggtc cggttcggta taacccattt cccgcgccag tgtggtcgcc tcggagaaac
    13681 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
    13741 acttcatcaa ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc
    13801 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
    13861 actgatggta gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
    13921 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggaa gtacagtcaa
    13981 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
    14041 acggctcttt ggcttgcgcc agttcttcct gccagttttc cagattaagg ccatgtacat
    14101 tggtgagcag tgccttcgag ttagcaacac cgcagacacg taagtcgata tgtttattct
    14161 tcaaccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
    14221 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
    14281 cgccagtggt cgcatcatcg ttattgacca cgacagagat tgagcgttca gaagatccct
    14341 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
    14401 tcccacgtaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
    14461 ccgccaacgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
    14521 gttcagctcg cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg
    14581 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat cgctgcaaag acgcgcgccg
    14641 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
    14701 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
    14761 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctgaaac tgggcgatgg
    14821 gggtaattgt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
    14881 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataaa
    14941 caccgtcaac atccgtccag atctcgcaac aatcggcgcg taaacaggcc gccagcaccg
    15001 cagcggagta gtcggaaccg ttgcgtccaa gcaccaccag ctcgcctttt tcattaccgg
    15061 cagtgaaacc agccatcagc accatgtggt cagccggaat gcggcttgcc gcaatacggc
    15121 gggtggattc agcaatatca acggtagatt cgaggtaatg ccccactgca agcagttttt
    15181 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
    15241 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
    15301 ccaacaaact gatgccatgc aggacatgtt ttatttgggc aaattcctgg tcgacgaaag
    15361 ttttcaattg tgccagcgga aatcccggct gggcggcggc gagtcccgtc agaagttcgg
    15421 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
    15481 caatcatcgc taccagatgg ttggtaattt tggcgggggc agagaggacg gtcgccacct
    15541 gcccctgcct ggcattgctt tccagaatat cggcaacccg cagaaaacgt tctgcatttg
    15601 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
    15661 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
    15721 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
    15781 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
    15841 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
    15901 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
    15961 tgaaaagctg aaaatcactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
    16021 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg
    16081 taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca atgtcatggc
    16141 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    16201 ttgtattaat gttgctaatt gatagcaata gaccatcact gcctgcccca gattgagcga
    16261 aggataatcc gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt
    16321 agtcagcccg gaatcttcgc gaccaaacac cagagcggca tggctcatcc atgaagattt
    16381 ttcctctaac agcggcacca gttctactgg cgtagcgtag taatgatatt tcgcccgact
    16441 gcgcgcagtg gtggcaacag tgaaatcgac atcgtgtaac gattcagcta gtgtcgggaa
    16501 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccaacggg cggctggctc
    16561 caggtgtgcc tgactatcaa caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    16621 cgccgctgcc ccaatatttt ctgctctggc gggtgcaacc agaataatcg ttatacgcat
    16681 attgccactc ttcttgacca aataaccgcg aacagggtga tcattgtcaa cttattacgc
    16741 gttgagaatt tacaaattct taacgtaagt cgcaggaaaa gccctttgct tagcttaaaa
    16801 aaagctaaac tatttcctga ctgtactaac gattgaattg ttaaaaaatg ctacatatcc
    16861 ttctgtttac ttaggataat tttataaaaa ataaatctcg ataattggat tcaccacgtt
    16921 tattagttgt atgaagcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    16981 agatacgatc atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    17041 aagcgtagtt ttattgggtg tcctgcccct cttagcctgt tatattgctg ttaaaatggt
    17101 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    17161 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    17221 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    17281 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    17341 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    17401 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    17461 acctggtgat catggatatc aatctgccgg gtaaaaacgg tcttctgtta gcgcgtgaac
    17521 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    17581 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    17641 aactgactat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    17701 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    17761 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    17821 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtcccg tgctgaactg ctgaagaaaa
    17881 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    17941 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    18001 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    18061 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    18121 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg
    18181 ggtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    18241 gctatcggca gagttgagca gtccccacat cacaccatcc agcaacaaca gcgcgagggt
    18301 aaacaacatg ctattacgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    18361 cgccccaatc agactggcga ttatccatgc cacagtaaaa ccggtatgtt cagaaagcgc
    18421 cagcaagagc aaataaaaca tcaccaatga cagccccacc agcaaatatt gcattgggtg
    18481 taaacgttgc gcggtgagcg tttcaaaaac aaagaatgcc ataaaagtca gcgcaatcag
    18541 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    18601 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    18661 accgagatta ttagcaaacc agctactttg ccattgcgct tgaaaacctg actcgctaac
    18721 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    18781 ggtcatttcg ctattacgcc cgccaggcac cacagaaaga tcgccggtac cgcttaaatt
    18841 cagcgccaca ttcagcttca ggttctgctt gcgccagtcc ccttcgggta aagggatatg
    18901 cacgccctgc ccgccttgct ctaacccggt gccgggttca atggtcagcg ccgtaccgtt
    18961 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    19021 tggcttgcct aaggtgatat ttggcgcgtc gagttcgcta agacgcgaaa catcgaaatc
    19081 ggcttttagc gttaaatcac tgtgccagac ctgaccggta tagatgccta tcttgcgttc
    19141 ttccacattc tgattgccat caaccatcaa tgactcaggt aaccaaaaat gaataaaact
    19201 tcgtttccgc tccacggttt tatcgtcttc ctgcaccgta taaagctcgg tcacaggaat
    19261 agcgatgagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcacatcttc
    19321 cacatcgcta cggtaatcgg cacgttcgac aatcacctgc cgaatcagca ttatcggaat
    19381 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    19441 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    19501 ctatgtgaag tgacggtgaa gtcgaagcga ggccagcacg ccaccttcct gcacgttgcg
    19561 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acaaacgcca accccagacc
    19621 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    19681 cgcgtagtca ggaatcccac taccggtatc cagcacctta agggtgacgt attcctgatc
    19741 cacttcggcg cttagcgtta tgcgaccgct ctcgggagta aaatcgatgg cgttatccag
    19801 taaattcccc agcgcctgct ccagtaacgc cggttcagca gcaacgttaa cctcagtagg
    19861 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    19921 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    19981 tgcctggcgt agtaacgttt ccaccagtgc ctgcattcgc gcattttgcg tcagaatgtt
    20041 gtcggtaaaa cgagccacca cttccggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    20101 acgaatcgcc gccagtgggc tttttagctc atgagttaac gcataaacat actgctcaat
    20161 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcaa
    20221 ctcgctacta ccgagatcgg ggagaggaac gggcttattg tcagtgacgg aatcagcata
    20281 gcgagtgagc cgggcaatag agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    20341 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctac gcttaatgac
    20401 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc
    20461 cataatcggc gcggcaacat acatcacaga actttcggga tcggcaggat tttgcaacgt
    20521 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    20581 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccctgcgcat cggtcatata
    20641 gacatgatat tcgttgcgca ctttgttaat gccaccgata ttggcgcgaa acgggcgatg
    20701 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    20761 gggacgcgcc agctccgcca gcaacgttgc ggtgtcgatt aacgtcccct cggttgctct
    20821 tcgcacgccc ggtttaactt ctttgacaaa aatagccaga acgaaccagg ccgccaccgc
    20881 caccagtaaa aaatagccca gcaacaaccg catgccgata cgcattacag gcccctcagg
    20941 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    21001 ttggcacgca gcgttttaat gtgggtatcg acggtgcgat cgtaggtgtc ctgcgcatct
    21061 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcccggcga cttgagtaac
    21121 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    21181 tgcgccgcag gttcattcaa ttcaaaatga ccaatacgga taacgggaga cggcgtcgag
    21241 aacttcttca cccgacgcag taaggtgcgc actctggcgc acacttcgcg aggtgaaaac
    21301 ggtttagcca cgtagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    21361 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    21421 aattcaaagc cgctgatatc aggcaaacca acatcgagaa tcatgacatc gggaacctgc
    21481 tggcgagctt tatccagcac aggcaagcct cgctcaaaga cctcgacgtc aaaaccttcc
    21541 tgttgcaaca tgtagaccag cgtatcggct atcccttgct cgtcttcgac taaccagacc
    21601 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    21661 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    21721 ttgcgtttag cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    21781 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga tagttcaatc
    21841 ggcccgactt gttgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    21901 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    21961 tcgtcaaagg cttcaacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    22021 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    22081 attaaatgct tgtatttcat atcgttacca tttttatcta ttacacgagc aattagttcg
    22141 tcacggtaaa taacatattg attaagaaca aaatctcaat acttttattt ccgtcaagac
    22201 ctaaaacata ctcgactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    22261 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    22321 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcat cccctgtcgc
    22381 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    22441 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    22501 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    22561 ctcaacagac atttacccgc gctttcaaga agcagtttgc ccagactcct gcactttacc
    22621 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    22681 ctatgccaga gcacaaattt gtcactctgg aagatacgcc gctgattggt gttacccaga
    22741 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgc taccagttct
    22801 ggcacgattt tctcggcaac tcgccaacca ttccaccagt gctttacggt ttgaacgaaa
    22861 cacgtccgag ccaggataaa gacgacgaac aagaggtgtt ctacaccacc gcgttagctc
    22921 aggatcaggc agatggctat gtactgactg ggcatccggt gatgttgcag ggcggcgaat
    22981 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    23041 acggaacgtg catgccaatg cttaacctga cgcgccgtaa aggtcaggat attgagcgat
    23101 actacccggc agaagatgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    23161 tgattccgat ccgtcgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    23221 tgtcccctgc ggtttcaacc acccagccag acgccagcca caagctttcc tgataatcca
    23281 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga
    23341 gaatcgtact caccaggcat cccagtgcaa taccatggct caccagtagc ggtcggcttc
    23401 cctgcggtaa gtcccggcaa gattccagtg ccgcattaac gcgatcgctg agctcctgca
    23461 ttgactcacc ttcaggaata cgcccgtcaa cggtgccatt gaccagttgc cgacgccagt
    23521 tctcttcttc ttcggtgagt gaatcgatat ttcttgtttc cagtacaccc atatttaatt
    23581 cacgcaggcg agaatcaaag atgatgtcac aaccgcaggc ctgggcgatg atttccgccg
    23641 tgcgccgggt gcgtcctaaa tcgctactga tgatatgcgt aatgccaagc tctttggcac
    23701 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    23761 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    23821 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    23881 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    23941 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    24001 ggaacagccc tttggcagtg aggaaacgcg cgctggcgca cgaaataggg tagccaatgc
    24061 ccgccgttta cttcctgagg ctgatttttg ggtggcgatt gaagctggca tcgatggtga
    24121 cagcactttc agttgggtgg tgatagaaaa cgccagccag cgcggcgagg cgcgttcggc
    24181 tactttgccg ttgcctgcgg tgattctgga aaaagtgcgt gaaggtgagg tgcttggtcc
    24241 cgtgatgtcg cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt
    24301 gtttaccgcc gggaaactca ctcgcaccag cgtctatcac caggcggtga tcctggcgct
    24361 gagtccgttt cataatgccg tgtatcagcc attgcaggcc tgattaggcg ttcacgccgc
    24421 atccggcatt cggtgcacga tgcctgatgc gccatgtcct atcaggccta caaaatcaat
    24481 cgcttttcag caacacgtct tccagccact ggcgcagctc gacgggcgcg gctttcagac
    24541 tattagatcc acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct
    24601 ggctcatttc gccgcgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc
    24661 gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat
    24721 tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg
    24781 aatagggtga ttgttgggcc atagtatgtc gccattgtta gcgggggaag caaaatgcct
    24841 cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgtgg atcagtaacg
    24901 acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat agcggtagta
    24961 agcgtcataa gccagcacgt ttttcacata accgcgcgtc tcggagaatg gaatactctc
    25021 gacaaatgcc actgcgtcga tacgccctgc gctgttgcca agccaggttc gcacccgccc
    25081 tggtccggcg ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac
    25141 atattgcagg taactggtgc caatgttgat gtttgtttcc ggatccagca attgcccagg
    25201 gctgctataa ccgggaatag agaacatctt caccgtatgg gtcgcggtac caggcataat
    25261 ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg cactctcctg
    25321 acgggcaatc gccatcgcat agctttgcgg gatctcctta ccactggtgt agcgtttgaa
    25381 aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccacagct tcccggcgat
    25441 cgttgcctga acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc
    25501 ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc
    25561 gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct
    25621 gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttcgccga tgcgttgtgc
    25681 agcaaccatc gggtagaaac cacgctgttg catgagttga tgcaaaatct cttttgcttc
    25741 agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt catctttctc
    25801 tttcgcttcc ataggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc
    25861 aagcgccatt cgcacacggc gttcaataag cgaagtagat tgcgaacgca taatcgcgtc
    25921 atcgcgccat ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc atgcgacaat
    25981 atcgcgcagc tcctgaatct gatcttcatt aagctgctgt gcctgagcaa gcgacgggat
    26041 catcagccgt gcattttcag catcctgccg cgccacactg gcaaacgcca ccgccgccat
    26101 ttgacgggta aaatcggtcg cgccggttgt acgcgcgaag gtcagtaccg tattagggtt
    26161 gttcgccagt gaaatgattg ccgaagcgat agtctggtaa tcggcaggca tctgccctgc
    26221 cagcactgtt accaggccgg tgttacccgc tttcatcgcc agacggatac gctctaaata
    26281 cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt
    26341 aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg
    26401 cccggtgttc catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc
    26461 cgggctaaag gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg
    26521 agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt
    26581 caccgccggt tgattcatca ggtcatcagt gatctggcgg tattccaggt agggataaag
    26641 tgggtaatct ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca
    26701 ggcctgcttg atttgcgcgt aacgactacg ctgctcatcc agtgagtcgg ctcgcgccac
    26761 gctgctgacc gtcagcagac agacaccgac agccaacagc cgccaggtaa cgtgtttggc
    26821 tttttccaca agcgcatcct ctaagtgtca atacatcaat gcagcgtcat gccgcgtaat
    26881 agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta
    26941 cggggtgatc gactggctgg gattagacag ggaaacaggc tacacttcgc ctttgaaaac
    27001 tcatcatcac gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc
    27061 atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt
    27121 tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc
    27181 tgcgcattat ggcgggcatt gataaagaca tcgaaggtga agcacgtccg cagccagaca
    27241 tcaagattgg ttatctgccg caggaaccgc agctgaaccc ggaacacacc gttcgtgagt
    27301 ccattgaaga agcggtttct gaagtggtta acgccctgaa acgcctggat gaagtgtatg
    27361 cgctgtatgc cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg
    27421 aagagatcat tcaggctcac gacggtcaca acctgaacgt tcagctggag cgtgcggcgg
    27481 atgcgctgcg tctgccggac tgggacgcga aaatcgctaa cctttccggt ggtgagcgtc
    27541 gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac
    27601 cgaccaacca cctggatgcg gaatccgtag cgtggctgga acgcttcctg cacgacttcg
    27661 aaggcaccgt ggtggcgatt acccacgacc gttacttcct cgataacgtt gcgggctgga
    27721 tcctcgaact tgaccgcggt gaaggtattc cgtgggaagg taactactcc tcctggctgg
    27781 agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga
    27841 tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg ccgtcagtca aaaggtaaag
    27901 cacgtctggc gcgctttgaa gaactcaaca gcaccgaata tcagaaacgt aacgaaacca
    27961 acgaactgtt tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc
    28021 tgcgtaaatc ctatggtgat cgtctgctga ttgactccct gagcttctcg atcccgaaag
    28081 gagcgatcgt cgggatcatc ggtccgaacg gtgcgggtaa atcgacgctg ttccgtatga
    28141 tctctggtca ggaacagccg gacagcggca ccatcacttt gggtgaaacg gtgaaactgg
    28201 cgtcggttga tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt
    28261 ccggcgggct ggatatcatg aagatcggca acaccgagat gccaagccgc gcctacgttg
    28321 gccgctttaa ctttaaaggg gttgatcagg gtaaacgcgt tggcgaactc tccggcggtg
    28381 agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg
    28441 acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc actggaaaac gccctgctgg
    28501 agttcccggg ctgcgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca
    28561 cccacattct ggattaccag gatgaaggta aagttgagtt cttcgaaggt aactttaccg
    28621 agtacgaaga gtacaagaaa cgcacgctgg gcgcagacgc gctggagccg aagcgtatca
    28681 agtacaagcg tattgcgaag taatgcgtaa aatgccggat gcggcgcacc agcatcgcat
    28741 ccggtattag ttaaatgatt aatgggaagc aagttcccgg aaaaaggcag gattttcttt
    28801 aattgtggca agtacttttg cagccaatcc cgtaggattt cttcgcttcg attcccagct
    28861 tttaatggta tccacactgg ttcccagagc ttttgccatc tcactttgag aaacgttgag
    28921 ttgctcacga atagctttaa catccgcaat ttcgtagcgg gtcacccgtg caggttcttt
    28981 taaaccttgt ttaatctcga cagcctcttc cagagaggtt ttcaactcat caaaaaaact
    29041 cattttatat ctcgcctttg agcagtttcg tcaacttctt tagttgagct ttttccgtat
    29101 ctgtcagact atctttggca tttatcggat aggccataat caaataaata atctcttccg
    29161 tcgccagaaa gtaaattatt ctgacactgc cactttttcc ctgtgaaccc gcagccattc
    29221 ggactttacg caatccacca gtctgctgaa tgagatcacc tttatcggga gttccgatga
    29281 gttctttttg taattccttt aattcatcat cggttgcaat ctgcttgatc tgacgcgtaa
    29341 acattggcgt ctctataaat attatcgcct tacccattcc ccctccctgg caggtacatt
    29401 gtacacctaa atggagtact aagtacactt ttaaatcagt aatcccactc aacatcttat
    29461 aaagatgatt atctttgctc ccccatcatc tcccgcacca gctccacgca gcgcaggaaa
    29521 cggctgtcgt aatcgtcctc ttcaacccgc acgaattcga tattgttctc ttccaacatc
    29581 tccaccagca agttctggaa ctctttgcga tccaccgaac tgccgaggct gcgtaaacca
    29641 tccgccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca
    29701 atcaacgcct gcacgaacgg atgctcacgc ccttcgtact ttttgcagaa cgcctgggta
    29761 gtgacaaaat cggtgtcgat aaacgccact ttattggcat atttcactgc aaaatcaata
    29821 tattgcgcgt gtcccagcgc gattttatcg tagtcggaat actgcaacgc aatctcatca
    29881 ccgccaaggt gtgaaaagac ataatcgcga ccatattccc atgcactggt agtgttgaag
    29941 atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccgcc aaggatcgcc
    30001 acggtacgca caaagaacgg cttaacttcc gtaggaatat attcccagta gcggaacggg
    30061 ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gcttcggatc gatcaacacc
    30121 gtctcgatcc ccagatgttc catatactgc ggcgcatcgg cttcttctga ggtgtagata
    30181 agatccggct gaatgccttt ttcggccata aactttttga tgccgttgct ccacacatcc
    30241 cagccgtgtg gatacggttc catgccctct tcgttaaaag catgaatgcg gatatttttt
    30301 tgatacttaa aggtctgcaa taaccagcgc agacgatccg gcacggttgg ctgctgcgac
    30361 atggcgctgt cttcgaacaa tgcgcggtcg cgggtatcgt caaaacccat aatgatatgc
    30421 agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccagt atgcagcggg
    30481 taaaacttac cgaatacgac accgatcgtt ttcttctgcc ggggaaattc aagtcccaaa
    30541 taacggtgca atgcctccag cttttgtgcg ctggggcttt tgattttggc attcagtaac
    30601 tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc
    30661 tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatctctc
    30721 attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca
    30781 tcccttccgg cgcttttttc ggcacgttag ctgccggaac aattgcccgg cgaaaaccgt
    30841 gtttcgccgc ttctgagatt cgttcctggc cgctgggcac cgggcggatc tcccctgcca
    30901 gcccgacttc accaaacacc accagatcct gcggtagcgg tctgtcacgc aggctggaaa
    30961 ccatcgccag cagtaacgct aaatcgacac tggtttccgt caccttcacg ccaccgacca
    31021 cgttaacaaa cacatcctga tcggccattt gtagaccacc gtgacggtga agcaccgcca
    31081 gcaggattgc cagacggttt tgctccagcc cgactgccac gcggcgtgga ttcgccatca
    31141 tcgagtgatc gacgagcgcc tgaatctcca ccagcagcgg acgcgttcct tcccacacta
    31201 ccatcactga gctaccggag gtcacttcat cgccgcgact taagaaaatt gccgaagggt
    31261 tgctgacttc acgcagcccc tgctcggtca ttgcgaagac acccagctca ttcaccgcgc
    31321 cgaagcggtt tttatggctg cgtaaggtgc gaaaacgtga gtcggcatca ccatccaaaa
    31381 gcaccgaaca gtcgatacag tgttccagca ctttagggcc agccagcgag ccatctttgg
    31441 ttacgtgccc gaccatgaca atcgccacac cgcgcgtttt ggcgaagcgc gtcagataag
    31501 ccgccgtttc acgcacctgc gccacgctgc caggtgatga ttgtacatcc gccatatgca
    31561 tcacctggat ggagtcaatt accatcagct tcggttgctc ttcttcggca atcaggcaga
    31621 tctgctcgat gctggtttcc gacagcatat taagattgtc agtcggcagg ccaaggcgat
    31681 gagcgcgcat tgccacctgt tgcagcgact cttcgccggt gacatacagc gttttcatct
    31741 gctgcgccag tttgcacagc gtttgcagca acagcgtgga tttccccgca ccagggttac
    31801 cgccaatcag aatggcgctt cccggcacca cgccgccgcc taatacgcgg tcgaactctt
    31861 taaatccggt ggaaaaacgc ggcagctctt caaggctgat gtcggaaagt ttctggactt
    31921 ttgccactcc ggcgctaccg gcatagccac tgagacgctc gttacgcgcc accgttggcg
    31981 acgctgcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct
    32041 gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg
    32101 ccacgatgta cctcgttaat gctgtgccgc ccgcaggatg acgggcgggc aattacttct
    32161 gattcaggct gccagagagg atgcagaata cccccatcag gtcagcgtga cggatggtga
    32221 cttccgtctt ttcattcact tttggcttgg catggtaggc aatccccagc cctgccgctt
    32281 tgatcatcgg caggtcattg gctccatcgc caatcgccac ggtctgcgcc agcgggattt
    32341 catactcctg cgcgaggcgg gtcagggttt tcgctttata ttgcgcgtca acgatgtcgc
    32401 cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggctg
    32461 tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg gaggcaatcg
    32521 ccactttcca gcccagcgtt tccagcttga gtaccagctg cgttaagcct ggcatcagcg
    32581 gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc
    32641 ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg
    32701 ccaccctctc gcccgttccg gccagtttgg caatttcatc aatacattca atctggatgg
    32761 cggtggaatc catgtccatc accagcaacc ccggcgtgcg caggtgtggg attttcccca
    32821 gcggcgcgac atccagttgc gcttcatggg ccaggcgcgt agcccgcgca gtgagtgaac
    32881 ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatagccg
    32941 cgcccagttt gctctggtat tgggtcagac gttgtttatc cagcccacga ccatacagca
    33001 gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa
    33061 gaggcagacc aggccataaa gagacatctt caggcaggtc gcaccaggta atgttaggca
    33121 ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc
    33181 cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa
    33241 actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc
    33301 gctgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta atccgcaact
    33361 ggaagaactg gcccgcaccc tggcgcggca ggtgacgcta aacgttgcac cgctgatgcg
    33421 taccgactca ccggatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag
    33481 ccgcattctc gacgcaggcg tatatgatga gcaaggcgat cttatcgccc gttctggcga
    33541 aagcgtcgac gtgcgcgacc ggctggcgct cgacggcaaa aaagcgggcg gctattttaa
    33601 ccagcagatt gtcgaaccca ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac
    33661 gctcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt
    33721 acgcctgatg ttgctgctct cgctggcaat cggcgtagtg ctgacccgca cgctgctaca
    33781 gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagta aaccggtgcc
    33841 ggaagaggaa gaaagcgaga aaaaagagtg acccattact acaagaaagg aaatcgttat
    33901 gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtaga
    33961 ggagtgtatt tttcgccaga tgccggctac gcagcgcgtt ctgtttctct ggcgcaatgc
    34021 cgacacggta gtgattggtc gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat
    34081 ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcag tgttccacga
    34141 tctcggcaat acctgtttta cctttatggc tggcaagccg gagtacgata aaaccatctc
    34201 cacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag cttccgggcg
    34261 taacgatctg gtggtgaaaa ccgccgaagg cgatcgcaaa gtctcaggct cggcctatcg
    34321 cgaaaccaaa gatcgtggct tccaccacgg cactttgctg ctcaattccg accttagccg
    34381 cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacctcagt
    34441 gcgttcccgc gtgaccaatc tgaccgaatt gctaccgggg atcacccatg agcaggtttg
    34501 cgaggccata accgaggcct ttttcgccca ttatggcgag cgcgtggaag cggaaatcat
    34561 ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc gccagagtag
    34621 ctgggaatgg aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttac
    34681 ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt
    34741 gtttaccgac agtctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg
    34801 tctgtaccgc gcggatatgc tgcaacagga gtgcgaagcg ctgctggttg acttcccgga
    34861 acaggaaaaa gagctacggg agttgtcagc gtggatggca ggggcggtaa ggtaactcgc
    34921 tgattaaaat atccagggaa ggaaatatga tcgatctgga aaaccaggaa cgggagatta
    34981 tcaatttaat gttcagccag ggtatatcct ggctaaccgc cgtgcgtatc aggcataaac
    35041 tatcacttgc tgaagtaagt aaaatgttag gtatctccat taattctcta aaacagatcg
    35101 aaaagacgga acggcttagt tcaaacatca aaagcaaaat ggcaggaatt tatggttgtc
    35161 caccagagtt gctgatatgc ccttcctgga tgactgctga acataaatga atagattcgt
    35221 gaatagatac ttgaatggat tttattttct atccatatga aaaataatag taaatctata
    35281 aatcagttgc tattgatgaa taaatactaa aaagccggag cagtctccca ctccggcttc
    35341 acacgacaat cgcctttcag caaaacacaa ttactcttta tcgcccagca gaacggattc
    35401 cagcgcgatt ttgatcatgt cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc
    35461 gtgagtgcgg atgtggtcag acacggtgca gatggtcagg gctttcgcgc caaattctgc
    35521 agcgacgccg tagataccag ccgcttccat ttccacgccg aggatgccgt atttttccat
    35581 cacgtcgaac atttcgccgt ccggagagta gaacaggtca gcggagaaca ggttaccaac
    35641 gcgagcatca acgcccagtg cttttgccgc gtctaccgcg ttacgcacca tgtcgaagtc
    35701 agcgatagcg gcaaagtcat ggtctttaaa acggatgcgg ttaactttgg aatcggtgca
    35761 ggcacccata ccgataacga cgtcgcgcag ttttacgtgc ggcagaacag cgccacagga
    35821 accaacgcgg ataattttct tcacgccgaa atcagtgatc agttctttgg tgtagatgga
    35881 gcaggacggg atacccatac cgtggcccat tacggaaatt ttgcggcctt tgtaagtacc
    35941 agtgaagccc agcataccgc gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc
    36001 agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa actacgtcag caaaatcgcc
    36061 catttctgca ttaatgtgtg gagtagccat cgttttatcc tttttgtgac atattaaagg
    36121 cggagtcact gctccgccca aatccatcag aacatggctt tgccatattc catatcagaa
    36181 gtaccaaaat attttgccag agtctggccg atatccgcga aggtttcacg atgccccagc
    36241 gaacctggtt tcactttcgg gccgtaaacc agtaccggaa tgtgttcacg cgtgtggtca
    36301 gtaccggtcc aggtcgggtc acaaccgtgg tcagcagtga gaatcagaat atcgtcatcg
    36361 cgcagcaaag acatcagctc cggcagacgg cggtcgaaca gttccagacc cgcagcataa
    36421 ccggcgacgt cgcggcggtg gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg
    36481 atggtgttat cacccgcttc tttcatctct ttgatggtgg cgtcaaacag cgcgtccagc
    36541 ccggtcgctt tcactttttt ggtgataccg cagttggcat agatgtctgc aattttaccg
    36601 accgaaacca cctggccgtg tttttcatca accagtttct gcagcacggt cggtgctggc
    36661 ggctcaacag ccaggtcgtg acggttacca gtacgctgga agttaccagc tttgtcgccg
    36721 ataaacggac gagcgataac acgaccgatg ttgtagccgc cgttggtcag ctcttcacgg
    36781 gcgatttcgc acagctcgta gagtttatcc aggccaaagg tttcttcatg gcaggcaatc
    36841 tggaacacgg agtcagcgga ggtatagaaa atcggcttgc cggttttcat gtgctcttcg
    36901 cccagttgat ccagaatgac cgtaccggaa gagtggcagt taccgaggta acccggcaga
    36961 ttagcgcgtt caaccagttt atccagcagc tcttgcggga agctgttttc gtgatcggag
    37021 aaatatcccc actcaaacag aaccgggaca ccggcgattt cccagtgacc agacggggtg
    37081 tctttaccgg atgacatttc gtgcgcccat gcgtacgcgc cgataacttc agcgttgcca
    37141 tccattcccg ccggaatgaa accggtagaa ccttcgtgtg ctttcgccag cccaagacgg
    37201 gtcagatttg gcagattgag cgggccttta cgaccgtggt cggcttcgcc tttggcacaa
    37261 gcttctgcga tatgacccag ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct
    37321 gtagcgccga tgccgaatga gtccagcacc ataataaatg cacgtttcat atgttctccg
    37381 tactcagtgc ttcaaattta aatgatcgga tcagtatacc gttattcgct gatacggcga
    37441 tagacagttg gtgtgctttc cggtgcttta tcggcaagtt taattgccgc tttcaccgct
    37501 ttcgccgctt cctgccagct gttttcgtct ttcgcgtgga taactgccag cggacgctga
    37561 ccgtctacct ggtcgcccag acgcgccata tcagtaaagc cgacgctgta atcgatggtg
    37621 tcagatgcct gacggcgtcc gccgcccatt gcaaccactg ccatccccag cgcgcgggta
    37681 tccatttcac tgacaaaccc ttcggtatca gcatagactg ctttcgtcag catcgctgtc
    37741 ggcagatact tcgcgtagtt ctcaacgaag tcggtcgggc ctttttgtgc cgctaccata
    37801 cgaccaaaga cttctgccgc tttaccgttg tccagcaccg cctgtaattt cgcgcgcgct
    37861 tcggcgtcat ctttcgccag tttgccggag atcagcatct ccacgcacag tgccatcgtg
    37921 acatcaaaca gacgcgggtt acgatattca cccgtcagga actgcaccgc ttcacgaact
    37981 tcaaccgcgt tacctgcact ggaggccagt acctgattca tatcggtgag cagcgcggtg
    38041 gtgcgcacgc cagcgccgtt agccacgcca acaatcgctt cggcaagggc ttcagagagt
    38101 tcgtaggtcg gcataaacgc gccgctaccc actttcacgt ccatcaccag cgcgtccaga
    38161 ccttccgcca gtttcttcgc cagaatagag gcggtgatca gcgggattga atccacggtt
    38221 gcggtaatat cgcgggtcgc gtagaaacgt ttatccgccg gagccaatga gctggtctgg
    38281 ccgataatcg ccacgccgac gtctttaata atttcgcgga aacggttgtc atccgggaaa
    38341 atgtcgaagc cagggatgga ttccagtttg tcgagcgtac cgccagtatg accgaggccg
    38401 cgaccggaga tcatcggaat atagccgcca caggcagcga ccatcggccc caacatcagc
    38461 gaagtcacat cgccgacacc gccggtcgag tgtttatcaa caatcgggcc attcagatgc
    38521 aggcttttcc agtcgagaac ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc
    38581 tcaggcattg tcatatcgtg gaagaaaatg gtcatcgcga gggcggcaat ctgccctccg
    38641 gagatagtgt tgtcgcgaat gccgttgata aagaaacgaa tttcttcatc actcagcgca
    38701 tgaccatcac gttttttacg aataatttct tgtgcgagaa acaaggtaac ctcccgtgga
    38761 aaagagacga ggtaatcggg gaaccggatg agcatcgctg catcatccgg caaatcaagc
    38821 cttagtagct gctggcgctc ttaccgtcgc cgtggcccag agctttcagc aggcttgcca
    38881 gcaagctgga agcaccaaag cggtagtgac gcgcatcagc ccagtcagca ccgaacagtt
    38941 catctgcaat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg cccgccggtt
    39001 tgaaaccaac ggttttttct acgcccatat cgcggatcac ttccatcatg atgcgcgcgc
    39061 tttccggcgt cgcattcaca gccactttac cggtagaggt tttgatgaag tcagcacccg
    39121 ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt tcgccggttt
    39181 caataatcac tttcagcagt acgttcgccg cagcgcaagc ctctttacag gctttcacca
    39241 ggtcaaaacc aacctgctcg ttacccgcca tcagcgcgcg gtacgggaac accacgtcaa
    39301 cttcatcggc accgtaggcg attgccgcac gggtttctgc cagcgcaatg tcgatgtcgt
    39361 cgttaccgtg tgggaagtta gttaccgtag cgatacggat ttccggggtg ccctgctctt
    39421 tcagagtttt gcgagcaatc gggataaagc gaggatagat acagatagcg gcggtattgc
    39481 cgaccggagt tttggcctga tgacacaggg cgatcacttt ctcatcggtg tcgtcgtcat
    39541 tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc agatcagtca
    39601 tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta gtattctaac
    39661 atctactccg caacacactt cgatgtacat cacaattaag gaaatctact tacaagtttg
    39721 catcactgta atgcgatctg gttcaaataa ttcactttca aatgaatgcg tcagaggtgg
    39781 caaacgtatc aggatcaaag tgaacattac aaaacttctt acaatggcgc atcaccttcg
    39841 gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca acgttgccag
    39901 caaattgtca ccagcccggt acttagcccg gagcagaagc gccattttct ggcactggaa
    39961 gcagaaaata atctgcctta cccacagctg cctgccgaag cccgccgcgc gctggatgag
    40021 ggggtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc
    40081 gattacgccc gttttctggc gcacggttcc gaatggctgg agctggaagg cgcgaaagac
    40141 cttgatgacg cactctctct gctgaccatt ctttaccacc atgtaccgtc ggtcacatcg
    40201 atgccggtct acctggggca actggatgcg ttgctgcaac cgtatgttag aattctaaca
    40261 caagacgaga tcgatattcg aataaaacgt ttctggcgtt acctcgacag aaccctgcca
    40321 gacgccttta tgcacgccaa catcggcccg tctgattcgc ccattacccg cgcgatctta
    40381 cgtgcagatg cagagctgaa gcaggtttca ccgaacctga cctttatcta cgatcctgat
    40441 atcacccctg atgacctgct gctggaagtg gcgaaaaaca tctgtgaatg tagcaaaccg
    40501 cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta cgggattgtg
    40561 agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg ccttaacctg
    40621 aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac tctaccgcac
    40681 tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta tcaacaatca
    40741 cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga acgttttgtg
    40801 ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga gaaagaagga
    40861 attgtcgcac gttacggtaa agaagccgct gcaaatgaag tgggttatcg catcagcgca
    40921 caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa acgcgccatg
    40981 ctacacgcac agtcggggat cagttccgat atcggtacca cgcctggcgc acgcttgccg
    41041 tatggtgatg agccagatcc gattacccat ctgcaaactg tcgccccgca tcatgcttat
    41101 tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg taatccgcag
    41161 gcgctggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt taccgccaat
    41221 gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc ggatttagaa
    41281 aaatatcgcg ccgaaggttc acgcaccaac actacctggc tgggagaaga agccgcacgc
    41341 aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca gatgcgcttt
    41401 agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt
    41461 tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga tggggcgctg
    41521 taatgattgc ggagagtgcg tgccgcagtg tccgcatcag gcgttgcaga ttgtcgacgg
    41581 caaagtggcg tggagcgcgg ctgtctgcga gcaatgtgat acctgcctga agatgtgtcc
    41641 gcaacatgct acgccaatgg cgcaatccat gagcgtggac gaggtgctta accatgtccg
    41701 caaagcggtt ctgtttattg aagggataac ggtaagcggc ggcgaagcca cgacccagtt
    41761 gccgtttgtg gtggcgctgt ttactgccat caaaaacgat ccgcaactgt gccatctcac
    41821 ctgcctcgtg gacagtaacg gcatgttgag cgaaaccggc tgggaaaaat tgctcccggt
    41881 gtgtgacggc gcaatgctcg atctcaaagc gtgggggagc gaatgtcatc agcaattaac
    41941 cggacgcgat aatcagcaga tcaagcgcag catctgtttg ctggcagagc gcggtaagct
    42001 ggcggaactg cgtttgctgg taatccctgg ccaggtggat tatttgcaac acatcgaaga
    42061 actggcggcg ttaatcaagg gacttggcga tgttccggta cgcctgaacg cgtttcatgc
    42121 ccacggcgtg tatggcgagg cgcaaagctg gccgagcgcc acgccggagg atgtggaaca
    42181 actggctgat gcgttatggg agcgcggggt gagccggtta atatttccgg cgctatattt
    42241 gtgacatgcc ggatacggcc tacggatcgg cacagacctg taggcatgat aagacgcgtc
    42301 aagcgtcgca tcaggcattg tgcgccagct gccggatgcg gcgtgaacgc cttatccggc
    42361 ctacggttcg gcacagactt gtaggcatga taagacgcgt cagcgtcgca tcaggcattg
    42421 tgcgccaacg gccggatgcg gcgtaaacgc cttgtccggc ctacggcacg ttaaacaacg
    42481 tataggtgtt attaagcaac acttcggcaa tctcatccgc cggttccggt cgtaactcgc
    42541 aaagcacggc aaaaacacgg gcggcctgtt cagggcgatt aggctgcccc tgaaaaccat
    42601 tgagcggcat atctggtgcg tcggtttcca gcaacaacga tgccagcggt aattttgcga
    42661 tgacatcgcg ggttttactg gcgcgtggat aggtgatagt cccgcctacg ccaattttgt
    42721 agccctgttg cacaaaccgt tcggcctgtt gcaggctgcc ggaaaaacca tgaaccacgc
    42781 cagtgcacgg taaatcgtgg cgtttaagat gcatcgccag tttgtcgtgc gtgcgtcgtg
    42841 aatgcaggat caccggcaga tcgtagcgtt tcgccagttt cagttgttcg tcgagtaacc
    42901 actgctgcct ctcaaattgc ggatcgtcgc caaagagatc cagaccgatc tcccccaccg
    42961 ccaccacctt cgccggatgc ctttccagcg cctgctgtag ctgatcaaga gacacatcgc
    43021 tatgtttttc caacatacca ggatgcaagc ccaatgcggc atacagcggt tgatattttt
    43081 ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt cgccggaaca atgatcttgc
    43141 ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc atcgccacta aacggcggga
    43201 aatcaaaatg gcagtgggtg tcgataaaac ggcaaatcaa gccagatcct cattgttaaa
    43261 tgtcgggtcg ttcgcctgcg gagcatcgct cacttcggca accagcgtat cgttggcgac
    43321 tggcgcaggc ggaataacga tcgactccgg cgtcaccacc ggcaccagat ggcggctcag
    43381 cggcgctttt tcagtcaata atttaccgac cgttgccaga aaataacgcc cgcataaacg
    43441 tccaagttta tagtcttcac gcagcgccgg aatccgactg ccgagcgcga tactatgcaa
    43501 tggcttcggc ggataaattt cgaatatccg cagctggccc ggtggtttct caataaattg
    43561 ctgaatctca cgatagcttg tttcatgatg ctgcaccaga ttgaccagcg gctgcaggct
    43621 actgtcaccc agccagcgtt ccatgcgttt gaaccactgc ggcgtgtagt acatttgtga
    43681 cggcacagtg cggatgacga ccaacgtttt tgctccctgc cttgccgcct ctttaaccgg
    43741 gatcgcatca ctgatcccac catccaggta gttaatacct tccagcgaca ctccgctacg
    43801 ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc agccagtttt gtttagttgg
    43861 caaaaagtaa ttcggcgcat agtcatcctg acgacaggcg cacatataaa acgatttgcc
    43921 gctgtcaaat aaccgcgctg cggtatccat ttgcaacggc atctggcttg cggtggcctc
    43981 caccagccaa tcgagatcga taagatttcc tccacggaca aagcgcaatg gatcgaaaaa
    44041 ttcgcgtttt gtggtgtaac gcatgatgac tttgcgcgca taaccgggct gattgcagat
    44101 atacgccgag aggttctgcg ccccggcaga tgtgccgaga taaagatcga aaggattaaa
    44161 ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa attccacgtt gtccgccgcc
    44221 ttcgcacacc agagcaatcc gtccaggctg gaacggcctt agcgacaacg gcgcaatatt
    44281 accaagcgtt acaggtattc gctgccccac ctctgccttc ctgtttttgt tgtgaaagaa
    44341 tgacaaagta acgcaattta ttattcgcta aaaccgcaaa agccagtccg ctggactggc
    44401 tttggataaa attttcttaa tggtctgaaa tctagggtcg ttttcggccc atgaacaaac
    44461 tcaccaggaa cagaataatc ccgacgacaa agacaatttt agctgcgcct gcagcagtac
    44521 cggccagacc accaaaccca agtgcggcgg cgattaacgc gataaccaga aatatgatgc
    44581 cccaacgaaa cataagcgtc tcctttacca tagttaatgt caccgcttga tatgagcgtg
    44641 aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg cgcctcccga caaagttcgg
    44701 gaggtcgaat tacgacgaat tacttagttt tcagatcatt tttaacgctt ttcacaccat
    44761 ctaccgcttt ggcgatactt tcagcacggt cgctctgtgc ctgagaatct acggtaccgg
    44821 agagctggac cacgccatcg gtggtttcaa ctttcacatg acgggaaggg acaatgtcat
    44881 ctgccagcag tttggctttg atttcactgg tagtggcggt gtcacccgcg tagcctttca
    44941 cagagccttc tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc acgccctcaa
    45001 cacctttcgc cactttcacc gcctcttcgg cctgagcctg gctttcaacg aaaccgctca
    45061 gggtcacgac tttttgatcg gttttgacag agatatcggt gctcttgatg ttgtcatggt
    45121 ccaccagggc tgccttcact ttcgcggtga tggcactgtc atccatgaaa ttaccgactt
    45181 tattcataga gctatcgact ttttgccctg cgctttcatt ggtagtctgc gcgttgtttt
    45241 ctgcgtaggc agagccggtc gcgacggcag aggtcaacat tacagccagc agagttttcg
    45301 aaatcttcag tcttgtcata gtcatcgatt tattcctgta tgtttgctcg taatttgagc
    45361 ctggcaacac gaggttgcat tgctgaatag ggagagactt caccctctac agaagtcaat
    45421 ggtcgccatc acaaaagcga tgggtgatga ataacgacca ttacagcctc ttaatcagtt
    45481 attaatatcg gtagaatgac aatcgacggc tttagatact gatatctacg cattgaacgg
    45541 tatttaacgc cgtcataaat gtcattactt tgttaaatat agatcacaat tttgaaaccg
    45601 ctcgggatat cagctaaaac ataagcaaaa gtgaatgttt taagaacatt ccgtaagcgg
    45661 ctaataagga agggaaattg acagggcgca gcggctaccg cgccctggag gctagaatta
    45721 atgctcgcgg gtctggtgga actgaacgtc cggataacgt tcctgcgcca ggcgcagatt
    45781 gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca gttggctttc
    45841 gttcttacgc ttgaactctt cgaatttctt cgcgtccgca cattctaccc agcgggcggt
    45901 ggcaacgttg actgactcat acactgcttc aacgttgtat tcgctcttca ggcgcgctac
    45961 caccacatca aactgcagca caccaaccgc gccaacgatc agatcgttgt tggagatcgg
    46021 acggaacacc tgcaccgccc cctcttcgga aagctgtacc agccctttga gcagctgttt
    46081 ttgcttcagc ggatctttca ggcggatacg acggaacagt tctggcgcga agttcggaat
    46141 accggtgaac ttcatcatct caccctgggt aaaggtatcg ccgatctgaa tggtgccgtg
    46201 attgtgcagg ccgaggatat cgcccggata cgcttcttca acgtgcgaac ggtcgcctgc
    46261 cataaaggtc agcgcgtcgg agatcaccac atctttcgcg gtgcgcacct ggcgcagctt
    46321 catgcctttt tcatatttac cggataccac gcgcataaac gccacgcggt cgcgatgttt
    46381 cgggtccatg ttggcctgaa ttttaaatac gaagccggta aatttatctt cgctcgcttc
    46441 tacggtacgg gtatcggtct gacgcggcat cggcgcaggc gcccactcca ccaggccatc
    46501 cagcatatga tcgacgccga agttacccag cgcagtacca aagaataccg gggtgatttc
    46561 gcccgcaagg aacagctctt tgtcgaactc attagacgcg cctttcacca gctccagttc
    46621 gtcacgcagc tgctgtgcca gatcttcacc aaccgcagcg tcgagatccg ggttattcag
    46681 ccctttaaca atgcggactt cctgaatggt gtggccttta ccgctctgat agagataggt
    46741 ttcatcttta taaaggtggt aaacgccttt aaacagcttg ccgcagccaa tcggccaggt
    46801 gatcggtgcg cagccgattt tcagctcgtt ctcaacttca tcgagcagtt ccatcgggtc
    46861 gcggatatca cggtcaagtt tgttcataaa ggtgaggatc ggcgtatcgc gcagacgggt
    46921 aacttccatc agcttacggg tacgatcttc tacacctttt gcggcgtcga taaccatcag
    46981 gcagcagtcc accgccgtca gggtacgata ggtatcttcc gagaagtctt cgtgccccgg
    47041 ggtatcgagc aagttaacca ggcagtcgtg atacggaaac tgcatcacag acgtagtgat
    47101 ggagatccca cgctgctttt ccatctccat ccagtccgac ttagcgtgct ggttagagcc
    47161 acggcctttt actgtaccgg cggtctgaat ggcctgtccg aacagcagca ccttctcggt
    47221 gatggtagtc ttaccggcgt ccgggtgaga aataatggca aaagtgcggc gcttcgccac
    47281 ctcttgcaaa taaggagaca acgtcataat tctttcttct tgagtaagcg gcatcgcgcc
    47341 gcgcttgttg aaaacgaaaa attgcggcta ttttacccat caacgggggg gaggcaatca
    47401 gtgtttacac aggagctgct ccagttcgtg caacgaagaa acggtccagg tgggcgcgat
    47461 gccttctggt tgctcgcgat tgtgcgcatt cagccagcag gtcgcaagcc cggcgttgat
    47521 gccaccgaga atatcggact cggcagtgtc accaaccatc agcacgcgtg aacggtcagg
    47581 attgcccgct tgttccagcg cataatcgaa aattttctta ttcggtttgg caacgccaac
    47641 ttcttcggaa atcaccagca aatcgaagta atcacgcagg cccgtgcgtt ccagacgtac
    47701 ctgttgcaag gcgctaaagc cgttggtgat gatgccgatt ttggcgttgc cacgaatggc
    47761 gttaagcagg gaaaccgccc ccggcaacgg cgtacagatt tccgccatcg cattaataaa
    47821 ggcctcgttg agtttacccg gttcgacgtt cagccgttcg gcccagctct caaaccgccc
    47881 gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa tccacccaca gtggcttgtt
    47941 aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg acgctgtaat caagaaacat
    48001 ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt tcatcggcat caaagaaaat
    48061 ccagtcccac ttcattattc caccttgtct tacatactga ttggtaacgc catgatgatg
    48121 gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc gaatcgtcgc ctcgttaaag
    48181 cctaaacttt cgtacagggc aatggcggca gcatttgagg cacggacttc cagccatagc
    48241 gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt ccagcagcgc ccttcccagt
    48301 ccctgacgct gatagtcagg atcgaccgca atattgaaca atgtagcttc atccagcacc
    48361 acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg ttaactgaaa gttgagataa
    48421 cgctcgccct ggttgctggc aaaggttttt tcactccacg gaaaggcgtg ggcgcgttgt
    48481 tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg aagaaatcgt gttcatatgt
    48541 gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt gcccgtaatt cggtgagcgc
    48601 cggtgatgcc acctgagcgc cttccagtga tagcggttcg tcagtaccca accgccagct
    48661 gttgcagcga ctgccttgcg gcagcatcgc gattttttct ggcgtcagtt gcagcacttg
    48721 atcggggctg acggttaacg cacgcagaac atcgctcacc agaggatcgg tcagggcggg
    48781 aagatcgttt gccaccatca ccagacggac gtgtggcggg atcgcaatgg caatctcccc
    48841 ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg cccagttgct gtaactgcca
    48901 gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag gggcgcaaat atagcaaatt
    48961 cgtcgatacc gcgccaacat atggctataa tcgccgccag tatcaattga ggagcattcc
    49021 atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc acagtgatga tttcgaacaa
    49081 agccgtattc tgtttgccgg agacttacag gatgacctgc ccgcgcgttt agataccgcg
    49141 gccagccgtg ctcataccca gcaattccac cactggcagg tgttaagccg ccagatgggg
    49201 gataacgccc gttttagcct ggtcgccacg gcggatgatg tcgctgattg cgatactttg
    49261 atttactact ggccgaagaa caaaccggaa gcccagttcc agttgatgaa tttactttct
    49321 ctgcttccgg tgggcacgga tatttttgtc gttggcgaga accgcagcgg cgtgcgcagc
    49381 gccgagcaga tgctggccga ttatgcgccg ttgaataagg tagacagcgc ccgtcgctgc
    49441 ggcctctatt ttggtcgtct ggaaaagcag ccggtatttg atgccaataa attctggggc
    49501 gaatacagcg tcgatggctt gacggtcaaa acgctgccgg gcgtgtttag ccgcgacggt
    49561 ctggatgtcg gcagtcagtt gctgctctcg acgctaactc catacacgaa aggtaaagtg
    49621 ctggatgtcg gctgcggcgc gggagtgctt tccgttgctt ttgcgcgcca ttcaccgaag
    49681 atccgcctca ctctgtgtga tgtttctgca ccagcggtag aagccagtcg cgcaacactt
    49741 gcgaccaacg gtgttgaagg tgaagtcttt gccagtaacg tcttttccga ggtgaaaggt
    49801 cgctttgata tgataatctc caacccgccg ttccacgatg ggatgcaaac cagcctggat
    49861 gccgcgcaaa cgctgattcg cggcgcggtg cgtcatctga acagcggcgg cgagttgcga
    49921 attgtagcga acgccttcct gccttacccg gacgtgctgg atgagacatt tggtttccac
    49981 gaagtgattg cgcaaaccgg gcgcttcaag gtgtatcgcg ccattatgac ccgccaggcg
    50041 aagaaaggtt gagtatctga tatatgtcgc cgcataccgt gtcggatgcg gcgtacacgc
    50101 cttctctgac cgacaaacga attatccaca attcgctgat ggacattttt ccagcaatta
    50161 caccgctgtc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    50221 ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaattgg tagacgcgct
    50281 agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaat
    50341 tatctttact tcttttcttg tctcttcact gttttatcac ccctgattca aattcattat
    50401 catcagcatg atgccagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
    50461 ctgtttgtga tacaacatca ccggcgtcaa taccaatccc atcagaatga caatccgcca
    50521 ttgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
    50581 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
    50641 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
    50701 taaacctatc gataggatta gcaataatta tcatttagat tactatcacg attatggcct
    50761 atcgttccgc accgctctat gaagatgtta tctggcgaac gcatctccag ccgcaggatg
    50821 ctgggcttgc acaagccgtg cgggcgacga tcgccgaaca tcgtgaacat ttgctggagt
    50881 ttatccgcct ggatgaaccc gccccactta acgccatgac gctggcgcaa tggtcatcac
    50941 ctaatgcgct aagttctcta ctcgcggtct attccgatca tatctaccgc aaccaacctt
    51001 tgatgatccg cgaaaacaaa ccgctgatct cactgtgggc gcaatggtat atcggcctga
    51061 tggtaccacc attaatgctg gcgctgctga ctcaggaaaa agcattagat gtgtcgccgg
    51121 aacatgtcca cgttgagttt cacgaaaccg gacgcgccgc ctgtttctgg gtcgatgtgt
    51181 gtgaagataa aaacgcaaca ctacattcgc cgcagcagcg aatggaaacg ttaatcagcc
    51241 aggcgctggt tccggttgtg caggcactag aagcaacggg agagattaac ggcaaactta
    51301 tctggagcaa taccggttat ttgatcaact ggtatctgac tgagatgaaa cagctgctcg
    51361 gcgaggcgac ggttgaatcg ctgcgctatg ccctcttttt tgagaaaacg cttactaccg
    51421 gtgaagataa tccactctgg cgtaccgtgg tgctacgcga cggcctgctg gtgcgccgca
    51481 cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
    51541 gaaagcactg ccggggaagt aaacccggca tcatgcggat taataaggat gcaacacatt
    51601 acttgtttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
    51661 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
    51721 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
    51781 ttctctctcc gtcgggctta acgtccggct ttggttaatg taccactgat tgttcagtcg
    51841 atcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
    51901 cgttgacgct ttacttaata caccgtccag cggtgatggc gataatgaac caatcagccg
    51961 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
    52021 ttcagtcagg caggataatc cctctctgcg cgcacttctc atggcagaaa aagaaaaaat
    52081 gaccgctgca aacgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
    52141 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatacgact
    52201 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
    52261 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
    52321 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
    52381 gcgctaatag ttttattact tttcttaagc aatgaagcta tctggttgat gccccatcct
    52441 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
    52501 aaatcatgag tttcagtcat tgccggagag gtcagcatgg cgctgatacg ctctgagtgc
    52561 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
    52621 gtcgccgtag ggcacatgag caattctact gcctgggaat accaggagcg tgacaccatg
    52681 aaaacccagt gaatttcccg atattgtgag attaaagagt aataatgctc acaaacggaa
    52741 cgtggatctt cactttcacc ggccaaatca gcaatgacta atccggaacg acgtaattgt
    52801 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
    52861 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
    52921 ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta
    52981 ttcaaggcat tcattcctta aatatttcca gcaaattaat agattaagcg aataaattat
    53041 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
    53101 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggag
//
LOCUS       DAZFRE010000024        52802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFRE010000024 DAZFRE010000000
VERSION     DAZFRE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.024"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(113..1777)
                     /gene="glnS"
                     /locus_tag="ACHKUI_001684"
     CDS             complement(113..1777)
                     /gene="glnS"
                     /locus_tag="ACHKUI_001684"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6597900.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1997..2758)
                     /locus_tag="ACHKUI_001685"
     CDS             complement(1997..2758)
                     /locus_tag="ACHKUI_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6597901.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2760..3746)
                     /locus_tag="ACHKUI_001686"
     CDS             complement(2760..3746)
                     /locus_tag="ACHKUI_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFO6597902.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTASKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3739..4974)
                     /locus_tag="ACHKUI_001687"
     CDS             complement(3739..4974)
                     /locus_tag="ACHKUI_001687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFO6597903.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTVIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            5144..6301
                     /locus_tag="ACHKUI_001688"
     CDS             5144..6301
                     /locus_tag="ACHKUI_001688"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFO6597904.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDVLGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6305..7192
                     /locus_tag="ACHKUI_001689"
     CDS             6305..7192
                     /locus_tag="ACHKUI_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6597905.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCMDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7252..8010
                     /locus_tag="ACHKUI_001690"
     CDS             7252..8010
                     /locus_tag="ACHKUI_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6597906.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERRNKKVSTLIETHESHSH"
     gene            8046..9215
                     /locus_tag="ACHKUI_001691"
     CDS             8046..9215
                     /locus_tag="ACHKUI_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFO6597907.1"
                     /translation="MSVALIRDGVIRPSENDSHMLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPVNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9212..9682
                     /locus_tag="ACHKUI_001692"
     CDS             9212..9682
                     /locus_tag="ACHKUI_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6597908.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9696..10976
                     /locus_tag="ACHKUI_001693"
     CDS             9696..10976
                     /locus_tag="ACHKUI_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6597909.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATILETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIDVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(11065..13011)
                     /gene="nagE"
                     /locus_tag="ACHKUI_001694"
     CDS             complement(11065..13011)
                     /gene="nagE"
                     /locus_tag="ACHKUI_001694"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6597910.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13344..14144
                     /gene="nagB"
                     /locus_tag="ACHKUI_001695"
     CDS             13344..14144
                     /gene="nagB"
                     /locus_tag="ACHKUI_001695"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6597911.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLSKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            14204..15352
                     /gene="nagA"
                     /locus_tag="ACHKUI_001696"
     CDS             14204..15352
                     /gene="nagA"
                     /locus_tag="ACHKUI_001696"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6597912.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15361..16581
                     /gene="nagC"
                     /locus_tag="ACHKUI_001697"
                     /gene_synonym="nagR"
     CDS             15361..16581
                     /gene="nagC"
                     /locus_tag="ACHKUI_001697"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6597913.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16629..17381
                     /gene="nagD"
                     /locus_tag="ACHKUI_001698"
     CDS             16629..17381
                     /gene="nagD"
                     /locus_tag="ACHKUI_001698"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6597914.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17637..19301
                     /gene="asnB"
                     /locus_tag="ACHKUI_001699"
     CDS             17637..19301
                     /gene="asnB"
                     /locus_tag="ACHKUI_001699"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6597915.1"
                     /translation="MCSIFGVFYIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            19682..19758
                     /locus_tag="ACHKUI_001700"
     tRNA            19682..19758
                     /locus_tag="ACHKUI_001700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19716..19718,aa:Met,seq:cat)
     gene            19769..19853
                     /locus_tag="ACHKUI_001701"
     tRNA            19769..19853
                     /locus_tag="ACHKUI_001701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19803..19805,aa:Leu,seq:tag)
     gene            19877..19951
                     /locus_tag="ACHKUI_001702"
     tRNA            19877..19951
                     /locus_tag="ACHKUI_001702"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19909..19911,aa:Gln,seq:ttg)
     gene            19986..20060
                     /locus_tag="ACHKUI_001703"
     tRNA            19986..20060
                     /locus_tag="ACHKUI_001703"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20018..20020,aa:Gln,seq:ttg)
     gene            20076..20152
                     /locus_tag="ACHKUI_001704"
     tRNA            20076..20152
                     /locus_tag="ACHKUI_001704"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20110..20112,aa:Met,seq:cat)
     gene            20201..20275
                     /locus_tag="ACHKUI_001705"
     tRNA            20201..20275
                     /locus_tag="ACHKUI_001705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Gln,seq:ctg)
     gene            20313..20387
                     /locus_tag="ACHKUI_001706"
     tRNA            20313..20387
                     /locus_tag="ACHKUI_001706"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20345..20347,aa:Gln,seq:ctg)
     gene            complement(20542..21717)
                     /gene="ubiF"
                     /locus_tag="ACHKUI_001707"
     CDS             complement(20542..21717)
                     /gene="ubiF"
                     /locus_tag="ACHKUI_001707"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6597916.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMAENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            21863..23287
                     /gene="miaB"
                     /locus_tag="ACHKUI_001708"
     CDS             21863..23287
                     /gene="miaB"
                     /locus_tag="ACHKUI_001708"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6597917.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            23440..24480
                     /gene="ybeZ"
                     /locus_tag="ACHKUI_001709"
     CDS             23440..24480
                     /gene="ybeZ"
                     /locus_tag="ACHKUI_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6597918.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            24477..24944
                     /gene="ybeY"
                     /locus_tag="ACHKUI_001710"
     CDS             24477..24944
                     /gene="ybeY"
                     /locus_tag="ACHKUI_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6597919.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            25034..25912
                     /gene="corC"
                     /locus_tag="ACHKUI_001711"
     CDS             25034..25912
                     /gene="corC"
                     /locus_tag="ACHKUI_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6597920.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            25937..27475
                     /gene="lnt"
                     /locus_tag="ACHKUI_001712"
     CDS             25937..27475
                     /gene="lnt"
                     /locus_tag="ACHKUI_001712"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6597921.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(27602..29479)
                     /locus_tag="ACHKUI_001713"
     CDS             complement(27602..29479)
                     /locus_tag="ACHKUI_001713"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO6597922.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYTLRESEQIVISIGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVGQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNNLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            29933..30841
                     /gene="gltI"
                     /locus_tag="ACHKUI_001714"
     CDS             29933..30841
                     /gene="gltI"
                     /locus_tag="ACHKUI_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6597923.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTP
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMSFELSDEMKALFKEPNDKALN"
     gene            31011..31751
                     /gene="gltJ"
                     /locus_tag="ACHKUI_001715"
     CDS             31011..31751
                     /gene="gltJ"
                     /locus_tag="ACHKUI_001715"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6597924.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31751..32425
                     /gene="gltK"
                     /locus_tag="ACHKUI_001716"
     CDS             31751..32425
                     /gene="gltK"
                     /locus_tag="ACHKUI_001716"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6597925.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32425..33150
                     /gene="gltL"
                     /locus_tag="ACHKUI_001717"
     CDS             32425..33150
                     /gene="gltL"
                     /locus_tag="ACHKUI_001717"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6597926.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33268..34203
                     /gene="rihA"
                     /locus_tag="ACHKUI_001718"
     CDS             33268..34203
                     /gene="rihA"
                     /locus_tag="ACHKUI_001718"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6597927.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(34248..34730)
                     /gene="ybeL"
                     /locus_tag="ACHKUI_001719"
     CDS             complement(34248..34730)
                     /gene="ybeL"
                     /locus_tag="ACHKUI_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6597928.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            34965..37547
                     /gene="leuS"
                     /locus_tag="ACHKUI_001720"
     CDS             34965..37547
                     /gene="leuS"
                     /locus_tag="ACHKUI_001720"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6597929.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPIADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            37562..38143
                     /gene="lptE"
                     /locus_tag="ACHKUI_001721"
     CDS             37562..38143
                     /gene="lptE"
                     /locus_tag="ACHKUI_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6597930.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMIQDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTLATPARVSTTLGN"
     gene            38143..39174
                     /gene="holA"
                     /locus_tag="ACHKUI_001722"
     CDS             38143..39174
                     /gene="holA"
                     /locus_tag="ACHKUI_001722"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6597931.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            39176..39817
                     /gene="nadD"
                     /locus_tag="ACHKUI_001723"
     CDS             39176..39817
                     /gene="nadD"
                     /locus_tag="ACHKUI_001723"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6597932.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSAQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            39841..40452
                     /gene="cobC"
                     /locus_tag="ACHKUI_001724"
     CDS             39841..40452
                     /gene="cobC"
                     /locus_tag="ACHKUI_001724"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6597933.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            40712..41029
                     /gene="rsfS"
                     /locus_tag="ACHKUI_001725"
     CDS             40712..41029
                     /gene="rsfS"
                     /locus_tag="ACHKUI_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6597934.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            41033..41500
                     /gene="rlmH"
                     /locus_tag="ACHKUI_001726"
     CDS             41033..41500
                     /gene="rlmH"
                     /locus_tag="ACHKUI_001726"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6597935.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            41531..43432
                     /gene="mrdA"
                     /locus_tag="ACHKUI_001727"
     CDS             41531..43432
                     /gene="mrdA"
                     /locus_tag="ACHKUI_001727"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6597936.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            43435..44547
                     /gene="mrdB"
                     /locus_tag="ACHKUI_001728"
                     /gene_synonym="rodA"
     CDS             43435..44547
                     /gene="mrdB"
                     /locus_tag="ACHKUI_001728"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6597937.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            44558..45646
                     /gene="rlpA"
                     /locus_tag="ACHKUI_001729"
     CDS             44558..45646
                     /gene="rlpA"
                     /locus_tag="ACHKUI_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6597938.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVIPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            45786..46997
                     /gene="dacA"
                     /locus_tag="ACHKUI_001730"
     CDS             45786..46997
                     /gene="dacA"
                     /locus_tag="ACHKUI_001730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6597939.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            47107..47370
                     /gene="ybeD"
                     /locus_tag="ACHKUI_001731"
     CDS             47107..47370
                     /gene="ybeD"
                     /locus_tag="ACHKUI_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6597940.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            47471..48112
                     /gene="lipB"
                     /locus_tag="ACHKUI_001732"
     CDS             47471..48112
                     /gene="lipB"
                     /locus_tag="ACHKUI_001732"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6597941.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            48371..48691
                     /locus_tag="ACHKUI_001733"
                     /pseudo
     CDS             48371..48691
                     /locus_tag="ACHKUI_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            48932..49897
                     /gene="lipA"
                     /locus_tag="ACHKUI_001734"
     CDS             48932..49897
                     /gene="lipA"
                     /locus_tag="ACHKUI_001734"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6597942.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(49997..50200)
                     /gene="tatE"
                     /locus_tag="ACHKUI_001735"
     CDS             complement(49997..50200)
                     /gene="tatE"
                     /locus_tag="ACHKUI_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6597943.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(50329..51117)
                     /locus_tag="ACHKUI_001736"
     CDS             complement(50329..51117)
                     /locus_tag="ACHKUI_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6597944.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICGSLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWGRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            51213..51596
                     /gene="crcB"
                     /locus_tag="ACHKUI_001737"
     CDS             51213..51596
                     /gene="crcB"
                     /locus_tag="ACHKUI_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6597945.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(51650..51859)
                     /gene="cspE"
                     /locus_tag="ACHKUI_001738"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(51650..51859)
                     /gene="cspE"
                     /locus_tag="ACHKUI_001738"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6597946.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(52034..52594)
                     /gene="pagP"
                     /locus_tag="ACHKUI_001739"
     CDS             complement(52034..52594)
                     /gene="pagP"
                     /locus_tag="ACHKUI_001739"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO6597947.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        12898 a        13751 c        13391 g        12762 t
ORIGIN      
        1 cggaattaat tcgcgaagaa aagcagaaaa aacgccgcag agcgcggcgt ttgggataat
       61 ttgcatattt cttgccggat gcggcgtaaa cgctctatcc gacaacgtaa acttactcgc
      121 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg
      181 tagaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
      241 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gactccgggt
      301 taatcaccga caggaaatca tccgcagcac cagggttcgg cacgctgaac agacgatcgt
      361 acaggcggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
      421 ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg
      481 tggtgatatt gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac
      541 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
      601 aatcggcgcg atcgatccaa atctcaccgc taaatggcac ctgacggctg cccatttccg
      661 gtttattcgg atggttcggc atggtaacca tttcgccttc gccctggtag ttttcgataa
      721 ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg agatcttcac
      781 ggatgcagga ttccagtgac gccatctcaa tggtgttgtc ctgcttggtc acaccaatgc
      841 gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccagaaatgg
      901 tcggcatacg cgggtcatcc cagccttcaa cgtgcttatc ggtcaccagc aggttcagct
      961 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
     1021 gaacaggaat ggtgatgttg tccagtaccc agtcgtacag acggcggttg tcctggaact
     1081 caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcag tgggtgaagt
     1141 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aacttaatgc
     1201 ggtacagcac cggatcgcgc atcacgataa acggcgaagc catgtcgatc ttcgcacgca
     1261 ggcaggcttt accttcttca aaaccgccgg tacgcatttt ctcgaacagc gccaggttct
     1321 cttcaacgct gcgatcgcgg tacgggctgt ttttacccgg ctgcgtcaag gtgccgcggt
     1381 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
     1441 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
     1501 agtggaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg atatcttctt
     1561 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
     1621 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
     1681 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
     1741 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
     1801 aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa
     1861 aaatggtttt gcgtaagata ttggggcgtt ttctgcgcta ctgccagcag atcctcgcgc
     1921 tggaaacgta aggccaaacg tcgccagcct gacgcattta ttaaattgcc ggatgagctg
     1981 gccgcagagt catagattag tcggctatga ttaaacgcgt cgacaaggac gcaagcgttc
     2041 tacgcgtctg cccatcaagc tgactatccg taataatctc tgtcagttca ttaagatgcg
     2101 cgatattaaa cagcgaccat gaaccgtatt ttgagctgtc agccaggaga attttgcgcg
     2161 aagcatgcgc gataagatcg cgttttagtg cggctttctc ttccgtgggt gctgtaatac
     2221 ccttttcaag atcccagctg ttacagctta aaaatgccac gtccggccag gtattttgca
     2281 gcaatttacg accatgttcg ccaatgcagg actggctgga gtcgtcgata cgtccgccga
     2341 taatcgtcac ttcaatttgc ttgaattcag ccaaaaaaag cgcgatatgc aggtcgcttg
     2401 tgatcacgcg aagcggtaaa tgtgtaatct gccgcgcaag ttcaatcatt gtcgtaccgg
     2461 catcaaggac gacggaatcc cccgctttga ccattgacgc cgctgcgctg gcgatggcat
     2521 gtttttccgc caggctgcgc tgcattttct catgggtagt cggttgggta ggaataaacc
     2581 ggttgagggt cacaccgccg tgtgtacggc tgataacacc ctgctcatcg agttttatca
     2641 ggtctcgacg aatcgtagcc ggcgacgcat tggttaccgc caccagctca tcaacggtga
     2701 ccagattatg gcttttcagg taatccatga tctgctctaa ccggttatat cccttcattt
     2761 tattctcgcg aaatctgcat tgctaactgg atggaaactg ccatgctctc agacttggct
     2821 tttcctgtcc aggcaatatc aaacgcggtg ccgtggtcgg cagaagtgcg aataaatggc
     2881 aacccggcgg tgatattcac cccatcataa aatccaagca gttttaaagg gatatgcccc
     2941 tgatcgtggt acatagccac taccatatcg aatagccctt catggcattg cataaacacg
     3001 gtatctggcg ggcacgggcc agtaacatca atatcttcgg cctgcattgc ttcaatcgca
     3061 ggcgcgataa tctcgatttc ctcagtgccg aataaaccat gttcccctgc atgtggattt
     3121 acacccgcca cggcaatacg cggacgctca ataccaacac gtttcaggaa gtggttcgca
     3181 acacgaataa ccgttttgac cctttcacca ttcaaggtat ccaggaattt acgcagtgag
     3241 atatgcgtcg aaatatggat aaccttgagc ttatcggtat agagcaccat cgcgtactct
     3301 ttgctggcgg taagatgggc cagcaattcc gtatgccccg gatagttatg cccgccaaga
     3361 tgcagcgctt ctttattcag cggcgcagtg gctatggctt tcacctcgcc tgataaggcc
     3421 agagccgttg cacgtttgat acagcgatac gctaaatcgc ctgccacagc ctgtaccact
     3481 ccgggtgtca gcgcatcggg gttagccagc ggctcatcca tcacgttaat cacgccggga
     3541 gcaaaatggg cgtcgcacac cttttgaatg acgcgtaact ctgcctgcgg cgtgatattc
     3601 atcgccataa tacgtcgcag cgtacgcaca cagcccacca ccaccaccgg ggcaccagca
     3661 agcccggctt cgctcaggga tttaataatg atctccgggc caatacccgc cgggtcaccc
     3721 atcgttaccg caataatatt attcactaca cttctcctcg ataaaattca gaacctgatg
     3781 caacgtggtc tcatcgccaa acccaccggc tttcgtcatc accgaacggg accagacacc
     3841 gcccagaaag tgaccgtatg gaacgcattg cgccaccttc cccgtaatac gaaatccggt
     3901 cgcgcctaac gcactggctg ttgccattgc gacatcacca ccagagagat aaagcgcatc
     3961 gggcattgtg cgaagcagaa cttgccgggt aagttcgcca agaaaccggc aaattttttc
     4021 cccaagtgca gcgcgactca tctgccaacg ctggcaaagg gtgtcgatct gatgtcttgc
     4081 tacagaatcg ttacaggtgt ggacaatgca gtggtcgcct ttctgcaagg cctgcacaat
     4141 acgcgcgtca tagtcactgg cattgcctgc cagaatgtgt tcaacatcga cgtatatctg
     4201 tgtgatacgt gggtgtgaat gaagtgtggc gatctgccgt tgagcgattt cgctcattga
     4261 accaataatg gccagcacgg tgggtggcgg cataatcgcg gagcgccggg caacggcatc
     4321 acaaatacca gcggagccga ccagcagcag cggttgttta gcctgcatca cagcggcagc
     4381 gatgcgatcc aggtcgctgt ccgtctgcgc atctatcacg ccaatccacg gttgctcata
     4441 agacaaatgg ctgagctgag aaaccgtaac gggctggcat ggaatgaccg tctgcaaccg
     4501 gatgatgtcc gcaattcggg cactcaatac cggggttttc ggatcgctgg caaactcggt
     4561 ttccgttatc cgcacaccgt ttacccaaca ctcaccggcc actgtggtcc ttcccgcctg
     4621 aggaaatgcg ggggcaacca ctgcgcccgt tataccgcat gccttcatca gggcttcaat
     4681 ttctgcgccg atattgcctc gcagcgtgga atctatcttt ttaatcacga gcggcgggtt
     4741 attcgccaga gggagatgac tcatcagcga ggttattttc atcgccgcca gcttaggatc
     4801 catcgcccgg ctgtcgctgt taatcaccca tacgctggca tcaccgcgat aaggcatttc
     4861 aaaggccaca tcaactgtat gccccacctg cgccaggctt acccccgcgt cgttggctcc
     4921 cgtgaaatca tcggcaacga cgagaatgct cggcttagtg attccctttt ccatcatcac
     4981 accaggttgt tttatgataa caatgattat attcaatcat tattgattaa atgtgagcaa
     5041 gctcaagtta ttaatcactc gaataaatta taaaattaga ccatgttacg taggctacgg
     5101 cattttgaat gattgaattc aatcaccaga agagaggtta tttgtggtaa ttatcaatag
     5161 cacgatactg agcggcgcag gcgctatccc ttccctgacg tcgctcttac ccgacatcag
     5221 aaaaatgctg ctggtcactg accgtaatat tgcgcagctc gacggtgtgc agcagattcg
     5281 cgccttactg gaaaagcact gcccgcaggt taacgttatc gataatgtgc ccgcagagcc
     5341 cacgcatcat gatgtgcgcc agctaatgga tgtccttggc gatgcctctt ttgatgtggt
     5401 ggtcgggatc ggcggtggca gcgtgttgga tgtggcaaag ctgctatcgg tgctttgcca
     5461 tccacaatct ccggggctgg atgcgctgct tgcgggtgaa aaaccgactc agcgggtgca
     5521 atcatggttg attcctacaa ccgccggaac cggctcagaa gccacgccga atgcgattct
     5581 ggcaatccct gagcaaagca cgaaggtggg tattatttcc caggtgctgt taccagacta
     5641 tgtggcgctt ttcccggaac tgaccaccag catgcccgcg catattgcgg cgtccacggg
     5701 cattgatgct ctttgccact tactggagtg ttttaccgcg accgtggcaa atccggtcag
     5761 cgataacgcg gcgctgactg ggttaagtaa acttttccgg cacattcaac ccgccgtgaa
     5821 cgatcctcag gatctgcgcg caaaactgga aatgctgtgg gcgtcttact atggcggcgt
     5881 agcgataacc catgcgggca cgcatctcgt tcatgcgctc tcctacccgt taggtggcaa
     5941 atatcatctg ccgcatggcg tcgcgaatgc catcttgctg gcgccgtgca tggcgtttgt
     6001 tcgcccctgg gcggtcgaga aatttgcccg ggtctgggat tgcattcccg atgcggaaac
     6061 cgccctgagc gcggaagaaa aatctcatgc cctggtgacc tggttacagg cattagtcaa
     6121 tcaactcaag ctacccaaca atctcgcgga tctcggcgta ccgccagagg atattgcctc
     6181 tcttagcgag gcggcactga acgtgaagcg ccttatgaac aatgtgccgt gccagcttga
     6241 tctacaggac gtacaggcca tttaccaaac actgtttccg caacatcaat ttaaggaata
     6301 aatcatgccg aaaaccattg aaggtgtatt aaccgctatc gtcaccccgt ttaccgcgac
     6361 cggcagtctg aatatcccgg cgttaaaagt gcaggtgaat cgtcaactgg aagcgggtaa
     6421 cgcgattttt tgcggcggca ccaacggcga gttttttgtg ttgaatgaac aggagaagct
     6481 cagcgttacg caaacctgca tggatgaagt ggctggtcgt gcgccggtcg tggcacacat
     6541 tggcgagatc tctacgcgcg aaacgattcg tctgggtcag cagattgaaa aacttggcgt
     6601 agatgccgtg tcggtcattg ccccttactt cgtaccgctg aaacaggaag agttgattgc
     6661 gcattacagc gcaattgccg acgcactgag cattccgatg tttctctata acatcccggc
     6721 ccgcactggc aatacgatcc agccagaaac cgcgcgtgct ctggcgtcgc accccaatat
     6781 cattgggatt aaagacagcg ccggaagcga cgaaagcctg aagggttttc tcgatgcggt
     6841 gcgcgacatt gacggcttta atgtgctcaa cggcccggat tcgctgatcc ataaagggtt
     6901 tgtcgaggga tgttcagcct gtatctctgg gctcgccaac atcgcgccgc gagaaatcaa
     6961 tgccatctgg tctcgcttcc atgccggtga tattgaaggg tcttatgaag cacaggagag
     7021 tgtaacgggc ctgcgtaccg atctttataa agtagcattc tccccggctg cggtgaaaaa
     7081 agcgttgcag cttatggggc atgatgttgg tgatagccgc tacgcggtta atttcacgcc
     7141 tgaacagatc agcgagattc aacaaattat tcgtcattat aacattaatt aaattaactc
     7201 tattatccag cccgctataa agtttagata aagagaataa aggatcaccc catgtttttg
     7261 tggtttatgc agccagatat ctggctcact ttaattaccc tcacctttct ggaaattatt
     7321 cttggggtgg ataatattat ttttctttca ctggtggtgg caaaacttcc gttatcacaa
     7381 caaaatatgg cgcgtaaact gggtttaagc tgcgcgatga ttatgcggat attattgctg
     7441 atatccattg cgtggctttc acatattacc cagccgcttt ttaccgtgct gacgctggag
     7501 atttcgttcc gcaccttgat tctgctcggg ggcgggctgt tcctgattta taaagcgata
     7561 caggagatac gcgaagagct gtcgcccgcg caggaagacg aacatggcag cggatcgcgg
     7621 caactgtcgt tgtgcggtgc cattgtacag ataatgctgc tcgatatcgt gtttagcctg
     7681 gattcggtga tcaccgccgt cggcctttct cagcatatat ttatcatgat tgccgccgta
     7741 atgattgccg tcggtgtcat gatgtttgcg gcgaaaacaa ttggtgattt cgttaacgcc
     7801 acgccgtcaa taaaaatact ggcgttaacc tttttattgt ttgtcggtgt attgctggtt
     7861 gccgatagtc tcaacattca tattgccaaa gagtatctct acttcgcgat atttttctca
     7921 ttaagtgttg agacactcaa tattatccgt gaaagaagaa ataaaaaagt ttcgacattg
     7981 atcgaaacgc atgaatcgca ctcgcattaa taaatctatt accaacttta agatggagtc
     8041 taactatgtc tgtagccctt attcgtgacg gcgtcatccg tccatccgaa aacgattcgc
     8101 atatgcttac cgccatgctg ccctcgtcct gcccgcaaaa ccacgcggcg aatattctgc
     8161 ctttgccgga tggcgcgtta atgtgcgtct ggtttgcggg aacgcaggaa ggtattgccg
     8221 atatttctgt ctggggatcg cgattgcccg ctggcggaat gcagtggagc gatgcggtca
     8281 aactttccca cgatgacacg cgttcagagc aaaacccggt gctgtttctc gcgccggtta
     8341 acgtgctgtg gctcctatgg accgcacaaa tatccggtaa tcaggacacc gccatcgtcc
     8401 gctaccgtaa gtcagacgac ctcggacaaa cctggggcga gattgccacc ctgcttgata
     8461 aacccggtac gttcattcga cagccaatca cggtgcttga taacggcaac tggctgctgc
     8521 ccgtctttta ttgccgtacc caacccggcg aaaaatgggt cggcaatgat gatatcagcg
     8581 cagtgaaaat ttccgctgac ggtggtcact cctggcgcga tgtcgaggtg ccgcaaagtc
     8641 ttggctgtgt tcatatgaat atcacaatgc tgcacaacgg tacgttggtt gctctctttc
     8701 gcagccgttg ggcagacaac atctatatca gccattccgc ggacaacggc gagagctggt
     8761 cagtaccgca agcaaccgaa ttgcccaaca acaattcgtc tattcaggta acgacacttg
     8821 ccagcggcga actggcgctg gtgtacaacg cgatgagtgc ggcaggcgca gtagaacgtc
     8881 gcgcgtcgct ttatgacgaa atcgacgatg gcgacgacag ccgcaaagaa ccgacagccg
     8941 tcggacgctc cgctttctgg ggggccccac gcgcgccgat gacggtagcg atttctgccg
     9001 atggtgggaa aagctggccg tggcggcgca atctcgatga aggcgacggc tactgcatga
     9061 ccaacaattc cctggaaaag ctaaatcgtg agttctccta ccccagcatc aaacaaagcc
     9121 cggacggtac gctacatatc gcttacacct ggtggcgtca ggccatcaag tacgtacgca
     9181 tctcccccga gtgggttaag gggcaggcat catgattatc ggtaatctca accatttgtc
     9241 gctggcgggg cttcctgcct gggtgagaaa tattctgctg cgcccggagt gttcgttatc
     9301 ggcgttatcc accagagagg acggtcgctg gcaacctgaa ggatgtcggt ggttctgcac
     9361 gctgggaact tcagacacgc aacccgccga actccgccat acggagtatc accatttatg
     9421 ggccgatata caggtggtga ttactggttg tgagggcatt aatgcgggaa cgcggcccat
     9481 cgctcgcgaa aacgatgaag aacgtaagcc cgatctgttt atcgccccat cccctgaaaa
     9541 cagtgtcgcc atcaccctac acgccggtga tttcgccgtc tttatgcctg aggaacctca
     9601 ccaggcgctg tgcgcaattg gcacggctac ttcaatacgc aaagcggtct ttaaagtacc
     9661 gcgcgacatg ctggaagcct aaaaggagag aaaaaatgac aatggaaatg atcctggcat
     9721 taggaatact ggtcctgatg atcgtgctga tcatgtcgga caaaatgcct tttggcgcac
     9781 cgccactgct tgcgtgttta ttgctggttg tatcgggact ttcgaccgtt caacaggctt
     9841 ttgccggatt cgtcaatcca agcgtggtga tgattgcagg gtttatggtg gtcatggcgg
     9901 cgctacaaaa aacccggctt atcagcaacg ttaaatctgc gatgataagc ctggtcaata
     9961 aaggcagtta ccgcagctat gggctgttgt tagtcattgt tatgctgggt gccagtcttg
    10021 ccggcaccgg agcgaccggt tattacgtgc taattctgtc gctggtatcc accattccgt
    10081 acagtaaaaa gctaccaaca tcgaaattaa tgatgcccct gggatttgcc accaaccacc
    10141 cgcttcttcc tatcaatctc gcgcttctct ttggcgttac ggcgaccata ctcgaaaccg
    10201 cggggtttca tcaggagatt tcgatggggc gctttgcgct ggtgaatctc atcatgtcag
    10261 cggctttcct cgcatggagt cttattgcct accgttttct tccggatcat cccattgcgg
    10321 atgcttccga ggatgcgctg gcggcacgcg aggaaacgtt taacgccctt cctgcctgga
    10381 aagaatattg cactattgcc gcattcgctg tcagcgttat cggcatgatg ctaatgaaca
    10441 ttctgggaaa tatcgccttc gttattccgg gtcttgcggg tgcgtttgtt ctgatgatcg
    10501 atgtactgga cttcaaagaa gttcgcgatc atatgggggc tccggtcatt ttaatgatgg
    10561 cgggggtgat cgatgtagct gatgcactgg cgggcaccgg ctttacggcg atggttggcg
    10621 atgccgttgc aggtgtgctc ggttctggcg tctcaccgtt tgtatttatt gtcgcctttg
    10681 ctttgcttac cagtacctgc gccaccttca cgggttcgaa tatggggtca gtatatattt
    10741 ttgcccccat cgccattgcc gcctgtacta gccttgggct taacccgacc gctgccgcga
    10801 ttgcggtggt gatttctggc tggaatggcg gctatatgcc tattgacgga atgccagcca
    10861 tgatcctcgg aatggggaaa tacaaacttc ccgaattctg ggtgttctct gtaccaatgt
    10921 atctcatccg cattctggcg ctttgtgcag gcgctgtttt tatcttccca atgtaatctc
    10981 agacaaaaaa agcggcagag aagatccctg ccgcttttta ttgttggatg cgacgctgcc
    11041 gcgtcgcatc agacataaag cagattactt tttgatttca tacagcggtg tttgacccgc
    11101 cacaacatgg ccctgagctt taatgataag gccgctgaaa tcgtcgatat tgctgcaaac
    11161 caccgggcta atcatcgagc gagcgttagc gttcaggtaa tccagatcca tttccagaat
    11221 cggttgccct gcgcttacct gcgcgccctc ttccaccaga cgtttaaagc ctttaccttc
    11281 cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag
    11341 acagaatgcg tggttggtgt tgaagatttt cacgattgtt cctgcggctg gtgatacgac
    11401 gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc
    11461 gtcaggaacc tgatccagtg ccacaacatc cccggtaatc ggcgatacca gctccgcgat
    11521 agataccgcg tttggtacag cctgcggttt tgctacagga gcggcagttg ccggagttgc
    11581 ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat
    11641 ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacccc
    11701 agaagcaccc agacgtttac acatcgcatc gttgacgcga gctgagtcaa ccacggtaag
    11761 gcgcagacga gtaatacagg cgtcaatggc tttcaggttg tcagtaccgc caaccgcagc
    11821 aatatagttg gttgccagtt gattgagacc ttcttcagta ttgctgttag cctcttcagt
    11881 aacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac
    11941 caaactgaac accacgaagt agatagcgaa gaagacaacc cccatcacca gcagcatcca
    12001 gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga
    12061 gaaagagaag ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag
    12121 cagtgcgtgc aggaggtaca gcagcggtgc aaggaacatg aacaggaatt ccagcggctc
    12181 agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg
    12241 acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat
    12301 cataatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg catagaagcg
    12361 gttgatatca ccgtggaaaa ccgttcccgc cgcgttggtg aattcaccaa tctggaacca
    12421 ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc
    12481 aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc
    12541 gtgctgtacc ggtggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc
    12601 ggtggcgatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat
    12661 atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac
    12721 acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac
    12781 cgcacccgcc agtgccgccg cacctgcgct gtctttcgac cagctggatg ccacgccgat
    12841 ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac
    12901 gttaagtaaa tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat
    12961 agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc
    13021 cctacgagaa tcctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattgtac
    13081 tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat
    13141 tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc
    13201 actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat caggtcggat
    13261 tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc caacggctta
    13321 cattttactt attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg
    13381 gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc
    13441 gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag
    13501 tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg
    13561 aatatgtcgg tctgtcgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt
    13621 tcttcgatca cgttgatatt ccagcagaaa atatcaacct tctcaacggc aacgccccgg
    13681 atatcgacgc cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc
    13741 tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc
    13801 tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt
    13861 tctttgataa cgatgttaat caggtgccaa aatatgccct gactgtcggt gttggtacat
    13921 tgctggatgc cgaagaagtg atgattctgg tgctgggtag tcagaaagca ctggcactgc
    13981 aggccgccgt tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg caactgcatc
    14041 caaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa
    14101 gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattatta tccctgccct
    14161 gacaccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca
    14221 gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg
    14281 cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact
    14341 gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt
    14401 acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc
    14461 caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct
    14521 gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga accaggcgtt
    14581 aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa
    14641 ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgagaacg ccgacgtgat
    14701 taccaaagtg actctggcac cggaaatggt tccagcggaa gtcatcagca aactggcaaa
    14761 tgccgggatt gtggtttctg ctggtcactc caacgcgacg ttgaaagaag cgaaagccgg
    14821 tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg
    14881 tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat
    14941 tgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaagcgtc tgaaaggcga
    15001 caaattgtgt ctggttaccg atgccaccgc gccagcaggt gccaacattg aacagttcat
    15061 ttttgcgggt aaaacaatat actaccgtaa tggcctttgt gtggatgaga acggtacgtt
    15121 aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgtggtat
    15181 cgcactggat gaagtgctgc gtatggcgac gctctatccg gcacgtgcga ttggcgttga
    15241 gaaacgtctc ggcacgctcg ccgcaggtaa agtagccaac ctgactgcat tcacacctga
    15301 ttttaaaatc accaggacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt
    15361 atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc
    15421 gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag
    15481 caaagtcagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg
    15541 ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc
    15601 accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc
    15661 accttgtttg atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt
    15721 acccagcaga cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac
    15781 cagcgcaagc tacgcgagct gatcgcgatt tcggtgatcc tgccagggct tgtagacccg
    15841 gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg gctggttgaa
    15901 gcactggaag agcgttttaa agtgacctgt tttgtcggcc acgatatccg cagtctggcg
    15961 ctggcagagc actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc
    16021 catcgcggaa ccggggccgg gattatctct aacgggcgaa tttttatcgg gcgcaacggc
    16081 aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc
    16141 aactttggct gcctggaaac tatcgccgcc aatgctgcca ttgaacaacg ggtgttgaat
    16201 ctgttaaagc agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc
    16261 tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag tgattgaata tgtcggtcgt
    16321 catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt
    16381 gctggagaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat
    16441 acccaggcgc tgaaggcgtt tcgtactaat ctaccggtgg tacgttctga gttggatcac
    16501 cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc
    16561 cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg
    16621 ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata
    16681 acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc
    16741 tggtgttgct gaccaactat ccttcgcaga ccgggcaaga tctggcgaat cgctttgcca
    16801 ctgccggtgt cgatgtaccg gatagcgtgt tttatacctc cgcgatggcg accgccgatt
    16861 ttctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg
    16921 aactgtacaa agccggtttc actattaccg atgtgaaccc tgattttgtg attgttggcg
    16981 aaacgcgttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg
    17041 cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcctgtggcg
    17101 cgttgtgtgc agggatcgag aaaatctccg ggcgcaaacc gttctatgtt ggaaagccga
    17161 gtccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacggtga
    17221 ttgtcggcga taacttgcgt accgatattc tggcgggctt ccaggccggt ctggagacga
    17281 ttctggtgct ttctggtgtt tcgtcgctcg acgatatcga cagtatgccg ttccgcccaa
    17341 gttggattta cccatcggtc gctgaaatcg acgttatctg aatagcaacc acgcctcagg
    17401 gcgtggtttc ttatttttac ccatcattaa aatcacccta ttcaataaaa cccgtcaaaa
    17461 attatcgatc catcaataaa tacgccatca caccctatcc tttttcacaa atcagcaatc
    17521 gccattacgt tttttatttt tccaccgcca aatcgcttgc gcaccgtgct gctttgcggc
    17581 attttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    17641 gttcaatttt tggcgtattc tatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    17701 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggcatt tatgccagcg
    17761 ataacgccat tctcgcccac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    17821 ctctctacaa ccaacaaaaa actcatgtgc tggcagtaaa cggtgaaatc tacaaccacc
    17881 aggcattgcg cgctgaatat ggcgatcgtt accagttcca gaccgggtct gactgtgaag
    17941 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    18001 ttgcctttgc cctgtacgac agcgaaaaag atgcatatct gattggtcgc gaccatctgg
    18061 ggatcatccc actgtatatg ggctatgacg aacacggtca gctgtatgtg gcctcagaaa
    18121 tgaaagccct cgtgccagtt tgccgcacga ttaaagagtt tccggcgggg agctatttgt
    18181 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tacgatgcgg
    18241 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    18301 gccatctgat gtctgatgtg ccttacggtg tgctgctttc tggtggtctg gattcctcaa
    18361 ttatttccgc tatcaccaag aaatacgccg cccgtcgcgt ggaagatcag gaacgctctg
    18421 aagcctggtg gccgcagttg cactcctttg ctgtaggtct gccgggttca ccggatctga
    18481 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    18541 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    18601 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    18661 ttaaaatggt gctgtccggt gaaggttctg acgaagtgtt tggcggttat ctatacttcc
    18721 acaaagcgcc gaacgctaaa gagttgcatg aagagacggt gcgtaaactg ctggccctgc
    18781 atatgtatga ctgcgcgcgt gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    18841 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaaccca caggataaaa
    18901 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgaa gcgtatctgc
    18961 ctgcaagcgt ggcctggcgg caaaaagagc agttctccga tggcgtcggt tacagttgga
    19021 tcgacaccct gaaagaagtg gcagcgcagc aggtttctga tcaacagctg gaaactgccc
    19081 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgggagatct
    19141 ttgaagaatt gttcccgctt ccgagcgccg ctgagtgcgt gccgggcggt ccttccgtcg
    19201 cttgttcttc cgctaaagcg attgagtggg atgaagcgtt caagaaaatg gacgatccgt
    19261 ctggtcgcgc ggttggcgtt caccagtcgg catataaata agacgtgtaa tattgccccg
    19321 gagaaatccg gggcaatttc agttactggg gcgcttgttg attctgatat gcagtaagga
    19381 aatgttccgg gtttgccaca tcgctgtttg acaactcatt actgtaggcc agtatttcat
    19441 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtatga caaaagaatg
    19501 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccatgctg
    19561 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    19621 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga
    19681 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
    19741 atcccgtcgt agccaccatc ttttttttgc gggagtggcg aaattggtag acgcaccaga
    19801 tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca
    19861 gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    19921 ttccctggtt cgaatccagg taccccagcc atattcttcg aataagcggt tcaccgcccg
    19981 gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc
    20041 gaatccaggt accccagcca tcgaagaaac aatctggcta cgtagctcag ttggttagag
    20101 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga
    20161 atgtgtcgaa tatgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta
    20221 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccaattta
    20281 ttcaagacgc ttaccttgta agtgcaccca gttggggtat cgccaagcgg taaggcaccg
    20341 gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct
    20401 cgcttcggcg agctttttgc ttttctgcgt atattcaatg ccgaatgcga tgttgactcg
    20461 tcttatcctt caaagtcgga tgcgacgctg cccgcgtctt atccgaccta cggttggcat
    20521 gcatccagca atgttgtgag gctacaaccc taacgcatat ttcagcgcct gacgtttcaa
    20581 cacgccagca cgctccgccg ccatcaaacc aagattacgc acaaaacgca gcggtggcag
    20641 attattgctg aatccggcat aaaacagatc cataccactt tgcatcatga agttatccgc
    20701 catacgccgc atctggtaac gcttgagcac aggataactg gcccacgctt cgccgtagct
    20761 gcgggcgttc accagaacgt caatcagggc atcgacatca cgataaccaa gattcactcc
    20821 ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagagcaa gccctggttg
    20881 cacatactgc aacgcatggc gacgtatcag cggaaacgca ccagcggcaa gcggtgttac
    20941 gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tgtagctgcg ccatattcat
    21001 attctgcaac tggcgaatgc gcgccggaga gtcataccac accagcgatg cccagttatc
    21061 aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc
    21121 gggatcgttc tcacactgta cgctaattaa catacacgac tgcgcatact gccaggcatg
    21181 aacgccaatt cccgccatct gtcgcacctg cgaatttgcg ccgtcggcac caatcaccag
    21241 cttcgcgagg atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatggcgatg
    21301 cagtgcaatc agcgaggttg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg
    21361 ccacaacgcc tgttgcagga cagtgttttc cgccatgtag ccaagcaacg gtagcttaag
    21421 ttcagcggcg tcaaacacga catgcgccgt ttcccactcc cacgtttcca gtctgcggta
    21481 aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac
    21541 cgaagccgcg ctgatcgccg agatccgcac gtccggctgg ctgtccgcaa caaacggcgc
    21601 aggctgggca tgttcaataa ccgttaccgt aaacccgtgc tgtgccagtc ccagcgccag
    21661 cgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt
    21721 caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata
    21781 acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct
    21841 actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca
    21901 gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca
    21961 actgaccgac gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga
    22021 gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa
    22081 tccagacctg attatcggcg tcggcggctg cgtggcatcg caagaaggcg agcacattcg
    22141 ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc gcctgccgga
    22201 gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat
    22261 cgagaagttt gaccgtctgc cagaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat
    22321 catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc gtggtgaaga
    22381 ggtaagccgt ccgtccgacg atattctgtt tgagatcgcc cagcttgcgg ctcagggcgt
    22441 gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga actacgacgg
    22501 caccaccgga acgtttgccg atctgctgcg tctggttgcc gccatcgacg ggatcgatcg
    22561 tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtatcg
    22621 cgacacgccg gagctggtga gcttcctgca cctgccggta cagagcggtt ccgatcgcat
    22681 tctgaacctg atgggacgta cccatacggc gctggagtac aaagcgatca tccgtaaact
    22741 gcgtgcggcg cgcccggata ttcagatcag ctctgacttc atcgtcggct tccctggcga
    22801 aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag
    22861 ctacagcttt atcttctctg cacgtccggg tacaccagcc gccgatatgg ttgatgatgt
    22921 tccggaagaa gagaagaagc agcgtctgta tattctgcaa gagcgcatta atcagcaagc
    22981 gatggcatgg agccgccgga tgctcggcac cacccagcgc attctggtag aagggacttc
    23041 acgtaagagc atcatggagc tttccggtcg cactgaaaat aaccgcgtgg ttaacttcga
    23101 gggcacgccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa
    23161 ctctctgcgc ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgcg tggcagaaac
    23221 accggaatcg gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca
    23281 gccgtaattc tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc
    23341 tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga
    23401 atgttcagct tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa
    23461 atcaccctgg agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc gtttgatgac
    23521 aacatcaaac aactcgaacg ccgtctcggc atcgaaatca atcgccgcga taaccacttt
    23581 aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat
    23641 gtcgatactg ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt
    23701 gcgattaaag aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa
    23761 gcggtcaata tcaagaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag
    23821 tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt
    23881 aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt
    23941 attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat
    24001 ttaagccaga aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg
    24061 ggctttgaga aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc accgctggcc
    24121 tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc ttgatgaaag ccagaacacc
    24181 accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc
    24241 accggcgacg tcacacagat cgacctgccg cgtaatacca agtctggttt gcgtcatgcc
    24301 atcgaagtgc tggctgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg
    24361 gttcgtcacc cggtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa
    24421 caaaaacgta aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga
    24481 gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga
    24541 gccagtttca gacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga
    24601 ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg
    24661 ataagccgac caacgtgctc tccttcccgt ttgaagtacc gcctggcatg gaaatgtcgc
    24721 tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca
    24781 aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt
    24841 acgatcacat cgaagatgac gaagcagaag aaatggaagc catcgaaaca gagattatgc
    24901 ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga
    24961 ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa
    25021 cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa
    25081 gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga
    25141 actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga
    25201 tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg
    25261 ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat
    25321 tgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat
    25381 tctgatggcg aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga
    25441 caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa
    25501 agagtttcgc tctcagcgtt accacatggc gatcgtcatt gacgaattcg gtggggtttc
    25561 cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta
    25621 tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc
    25681 actggcttct attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaggt
    25741 tgacactatc ggtggtctgg tgatgcaggc atttgggcat cttccggcgc gtggcgaaac
    25801 catcgacatc gacggttacc agttcaaagt ggcgatggcc gacagtcggc gtattattca
    25861 ggttcatgtc aaaatcccgg atgactcacc ccagccgaag ctggatgaat aaaaccgaaa
    25921 ctggatagat aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct
    25981 gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc
    26041 tgcggcgatt atttcgctga tggggcttca ggcgttgacc tttaaccgcc gtccactcca
    26101 gtctgccgct attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt
    26161 ctatgtcagc atcgcgacct ttggcggaat gcctggcccg gttaacatct tcctggtggt
    26221 actgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct
    26281 gtggccgaaa accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac
    26341 cgagtttctg cgcggttggg tactaacagg cttcccgtgg ttacagttcg gctatagcca
    26401 gattgatggc ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct
    26461 gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct
    26521 ggtggtggcc gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc agtggtttac
    26581 cccgcaaccg gaaaaaacta ttcaggtttc gatggttcag ggcgatattc cgcaatcgct
    26641 gaaatgggac ggagaccagc tacttaatac gctgaagatt tactacaacg caacggcacc
    26701 gctgatgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat
    26761 taatcagcaa ccgttcctca aagctctgga cggtgagttg cgcgataaag gtagctcgct
    26821 ggtcaccggg attgtcgacg cgcgtctcaa taaacagaac cgctacgata cctacaacac
    26881 catcatcacg ctgggtaaag gtgcgccgta cagctacgaa tcagccgatc gctataacaa
    26941 aaaccatctg gtgccgtttg gcgagtttgt cccactggag tcgatactgc gtccgttagc
    27001 accgttcttt gatttgccga tgtcgtcatt cagccgcgga ccatatattc agccaccgct
    27061 gtcgttgaat ggcattcaac ttactgcggc catttgctac gaaatcattc tcggcgagca
    27121 agtgcgcgat aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg
    27181 gtttggtaag tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga
    27241 gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca
    27301 gggtgagatt caggcgatga tcccgcagtt cacccgcgag gtgttgacca ctaacgtgac
    27361 accaaccacc ggactcacac cgtacgcacg aaccggcaac tggccgctgt gggtgctgac
    27421 ggcgctgttt ggttttgccg ctgtactgat gagtctgcgt gctcgcaaac gttaatgtgc
    27481 ctgatgcgcg acgcttatca ggcctacgcg atttttacaa tatgttgaat tttcgcgatt
    27541 ttgtaggata aagcgtactt tttcaacacg ctctggggca tgattatcat gccctttttt
    27601 cttacggttc agggatacaa gccacatcgc catattgggg atgccgaata attgcctgcg
    27661 gatcaagcga gcgcgtcctg tccatttgga ttaacctgtt cttcgccata acaaatatct
    27721 ttctaccatc cgctgaaaaa cgaagattat taacgtagct ggcgggataa acgccaataa
    27781 tctgcccggc attaatattg agcaggatga cataaccttg caggccatat gacaccaacg
    27841 ctaaatactg accgtccggg ctaataccct caacggaata aacgctcctg ctacaccatt
    27901 gcgtcataat gctggcttgt tgatcctcag ctcttatacg tgaaatcccc tggctatcgg
    27961 caaaaataat ctcaccattt tttggtcgga atcccatcgc agaaacgaca ccacgcgcat
    28021 cacctgcggg aaagtcttct ttctgatgcg tgatggtatt gatccgcacg atgttacccc
    28081 tgcctgctcc cggaaccagt aatgtttttt catccgggga aagtgccata acccaggcgc
    28141 ctggtttacc gctcatcccc gtcccgtcgt atttctccag cgggatatcc gccacatgct
    28201 gataagccac cagatcaatc gctgacacac tattgctggt gcgattcatc acccaggctt
    28261 ttgtgccatc acgcgagagg ataaggctgt caggatgggc gccggtggta atcgactgaa
    28321 taatctcccc cgtcgccacg ttaatgaccg ataacgcatt cgcttcaaat gaagtcgcat
    28381 aaatcaactt ttcatccagg cttaatgcca gcgatctgac gccctgatct ttacagccac
    28441 cacaatgttt gactgacttt tctttcgcta tgggcgctgt aaaggtatgc aggattttct
    28501 tttgtccgag gtcgaaaaca acaatgctgt tcgtgttttc aattgcggca tacaacgtat
    28561 taccatcccg actgatcgcc atatcatgga tatcggcaat ctcagcgagt aaatcaccac
    28621 tggcctgctc acgacttacc ggagcggata atgtttccct gagggcgttg cgctcctcca
    28681 ctaactgctg ctttttttgt cgtcgctctt gttcaatctc ctgcggataa aacgcttcac
    28741 gcaggctttg cccgacctgt aaaactgatt catcaatctg ctgctgcaca aaccagatag
    28801 tcccggtcag aagaagagtg accgcaacaa taatcccgcc ttccgtaacg agccgatttt
    28861 gcccaactaa acgcgcactc agccaaccca acgcgccccc cgcaatcagg ccgccaatgt
    28921 ggcaagcgtt atctattcct gtttgccggg taccatacaa cagtgtcagc gccaccatac
    28981 ccaacagagg aaatactcgc cgctggtttt tatggtgtgt gcccgcacct gaagcaagct
    29041 gtgttgctat cgcagcgccc gctattccca taattgcccc ggatgcccca atgctgatga
    29101 ctatctgctc actttcgcgt aacgtataac aggcgctgat cagcccacca aagatgccgg
    29161 agaataacca gataatcagg agtcgccatt tccccagtat ccgttccgcc actgtcccca
    29221 ccaccaggag tgcaaacata ttcatcagca aatgggaaaa actactgtgc agaaaaacac
    29281 tgctcaataa gcgccaggat tcatccgaca acgtgagaga agcaacgtta gcgccgagat
    29341 ggatgagtgc ggattgatcg acttcttcgt aactcatcca ttgtgcgagg taacaaaata
    29401 tcccaacaca gagcgcaaaa aggatcaggg ttattgcggg taattgaaca tttaacggct
    29461 taaccgaaga tgcagacata aggtgattct tccttgaaaa gactagtaaa aagcattttc
    29521 ccgagtgtag ttatcatccc ggcaaacgtc ccacaccgaa aagttaacac tgtccgaaat
    29581 atgacttttt caaactgaaa ttacaatcgt aattaattca cccctaagta ttttttggca
    29641 tgtaagttgc tttatttact gtgtaagcaa tgaaatcagc cgcaacgcaa ccgggttgca
    29701 ccaccacaaa tcagcggtag caccaaaaag gtgcggttta agaattttgc ttcggaatgg
    29761 tgcggcgcac ctcgcaaaaa taaacaataa aacaacaata tctttacatt tatccaaact
    29821 aaatgttacc taacaaacac aacactgcac aataaagtcg cagatgataa caacacaaac
    29881 actcacaacg ggtatccatg cgtttttaac gcagaagata aaggagttgg atatgcaatt
    29941 acgtaaacct gccacagcaa tcctggccct ggcgctttcc gcaggactgg cacaggcaga
    30001 tgacgccgcc ccggcagcag gcagcacgct ggacaaaatc gccaaaaacg gcgtgattgt
    30061 cgtcggtcac cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt
    30121 gggctactcg caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa
    30181 accagactta caggtgaaat tgattccgat cacctcacaa aaccgtattc cactgctgca
    30241 aaacggcact ttcgattttg aatgcggttc taccaccaac aatgccgaac gccaaaaaca
    30301 ggcggctttc tctgacacta ttttcgtggt cggtacgcgc ctgctgacca aaaagggcgg
    30361 cgatatcaaa gattttgccg acctgaaagg caaagccgta gtcgtcactc ccggcaccac
    30421 ctctgaagtt ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag
    30481 cgccaaagat cacggtgact ctttccgtac cctggaaagc ggtcgtgccg ttgcctttat
    30541 gatggatgat gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga
    30601 aatcgtcggc aagccgcagt ctcaggaagc ctatggttgc atgttgcgta aagacgatcc
    30661 gcagttcaaa aagctgatgg atgataccat cgctcaggtg cagacctccg gtgaagcgga
    30721 aaaatggttt gataagtggt tcaaaaatcc aattccgccg aaaaacctga acatgagttt
    30781 cgaactgtca gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta
    30841 attacaagaa ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac
    30901 tatacgcctg gtggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc
    30961 ttcgagggta gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag
    31021 actggaactg gggtattttt ttacaacaag ccccgttcgg caacaccacc tatcttggtt
    31081 ggatctggag tggctttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt
    31141 tcctcgtcgg ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc
    31201 tgggtacgtt gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct
    31261 ggtacctggt gatcccggag ctgctgccag agaaaatcgg catgtggttt aaggcggaac
    31321 tggaccctaa tattcaattt ttcctttcat ccatgctctg cctggggctg tttaccgccg
    31381 cccgtgtttg cgaacaggta cgcgccgcta ttcagtcgct gccgcgtggg caaaaaaatg
    31441 ccgcgctggc gatgggtctg acgctaccgc aggcttaccg ctacgtactg ctgcctaatg
    31501 cttatcgcgt tatcgtcccg ccgatgacct cagagatgat gaacctggtg aaaaactccg
    31561 ccatcgcctc gactatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgatt
    31621 actcggccca cgcctgggag tcgtttaccg ccatcacgct ggcgtatgta ttgattaacg
    31681 cttttatcat gctggtgatg acgctggttg aacgtaaagt tcgtctgccc ggcaacatgg
    31741 ggggcaaata atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct
    31801 cgacgggctg gtgatcacgc tgaaaatcac cgtcacggca gtggtgattg gtattttgtg
    31861 gggcaccatg ctggcggtga tgcgcttgtc cagctttgcg cccgtagcgt ggtttgccaa
    31921 agcctacgtt aacgtattcc gctcgattcc tttagttatg gttttgctgt ggttttacct
    31981 gatcgtaccg ggttttctgc aaaacgtgct gggattatca cccaaaaatg atatccgttt
    32041 aatttcggcg atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg
    32101 tgctggtatt caaagtattt ctcgtggtca gtcgagcgcc gcgctggcgt tgggaatgac
    32161 tcactggcag tcgatgaaac tgattattct gccgcaggcg ttccgcgcga tggtgccgct
    32221 gttgctcact cagggcatcg tactgtttca ggatacctca ctggtgtatg tgttatctct
    32281 ggccgatttc ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggttgagat
    32341 gatcctgttt gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag
    32401 ctacttgaaa agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt
    32461 cactttcagg tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt tgtggtggtt
    32521 tgcggtccgt ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaaccg
    32581 gtgcagcaag gtgaaatcac cgtcgatggc atcgtggtta acgacaagaa aaccgatctg
    32641 gcgaaactgc gttcccgcgt cgggatggtg tttcagcatt tcgagctgtt cccccatctg
    32701 tcgattatcg aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcaa taaagcgccg
    32761 gctcgtgaaa aagcattgaa attgctggag cgcgtcgggc tttccgcaca tgccaataag
    32821 tttccggcgc agctttccgg cggccagcag cagcgtgtgg cgatcgctcg cgcattgtgc
    32881 atggatccta ttgccatgct gttcgacgaa ccaacgtcgg cgctggatcc ggagatgatc
    32941 aacgaagtgc tggacgtgat ggtcgaactg gcgaacgaag gaatgacgat gatggtggtg
    33001 acccacgaaa tgggctttgc ccgtaaagtg gcgaatcggg tgatctttat ggacgagggt
    33061 aaaattgtcg aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca
    33121 aaagacttcc tcgcaaaaat cctgcattaa tcactctggc gcgcatactc aattgcgcgc
    33181 catctcacgc tttctcgttt cgtccctcgt atctgttaca tcccggcgtt acccttgtcg
    33241 caaagaaaca cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca
    33301 ggtcatgacg acgctatcgc aatcgttctc gccctcgcct caccagagct tgatgtcaaa
    33361 gcaattacgt cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt
    33421 atgctgacct tgcttaatcg caccgatatt ccggtagcaa gcggtgcggt aaaaccgtta
    33481 atgcgtaatt tgattattgc ggacaacgtg cacggcgaaa gcggtctcga cggcccggca
    33541 ttaccggaac cgacgttcgc accgcaaaac tgcacggcag tggagctgat ggcgaaaacg
    33601 ctgcgtgaaa gtgaggaacc tgtcaccatt gtttctaccg gaccgcaaac taacgttgcc
    33661 ttgctgctca atagccaccc ggaactgcat agcaaaattg cccgtatcgt gattatgggc
    33721 ggcgcaatgg ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg
    33781 gaagcggcag aaattgtctt ccagtcagga attccggtgg tgatggccgg tctggatgtt
    33841 actcacaaag cacaaatcca cgttgaagac actgagcgtt tccgcgcgat tggcaaccct
    33901 gtttcaacca ttgttgccga actgctggat ttcttcctcg aatatcacaa agacgaaaaa
    33961 tggggctttg tcggcgcacc actgcatgac ccatgcacca tcgcctggct gttgaaaccg
    34021 gagttgttta ccactgttga gcgctgggtt ggcgtggaaa cacaagggaa atatacccag
    34081 ggtatgacgg ttgttgatta ttattatctg actggcaata aaccgaatgc caccgtaatg
    34141 gtcgatgttg atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct
    34201 taatcactcg ccggatgaca tcacgccatc cggcaccact catttcatta cggctcgaac
    34261 gggcgtctct ggaactgatc atgaccacat ttcgggcata gcgtcagcac ttccggtgta
    34321 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc taccacttct
    34381 ccgctgtgat aaaccccgtg atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc
    34441 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    34501 acgctgtcag actcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    34561 cgtctgacag ctcgcgtcag ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt
    34621 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    34681 tcgctcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    34741 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    34801 ccacaaactc tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgagtgcgc
    34861 gaaaggctgt tttggcgcgg gcgtttgggc tatgctatgc ggatctgaaa aaccacatca
    34921 acgctacatt tgtagcagta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    34981 gcccggaaga gatagaatcc aaagtacagc ttcactggga tgagaagcgc acatttgaag
    35041 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    35101 gtcgactaca catgggccac gttcgcaact acaccatcgg tgacgtgatc gcccgctacc
    35161 agcgcatgct gggcaaaaac gtcctgcagc caatcggctg ggacgcattt ggtctgcctg
    35221 cggaaggcgc ggcggtgaaa aacaacaccg cgccagcacc gtggacgtac gacaacatcg
    35281 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    35341 cgacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgaa ctgtataaaa
    35401 aaggcctggt atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    35461 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgagcgta
    35521 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    35581 tggataaact ggatcactgg ccagacaccg ttaaaacaat gcagcgtaac tggatcggtc
    35641 gttccgaagg cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    35701 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtggct gcgggtcatc
    35761 cgctggcaca gaaagcggcg gaaaataatc ctgaactggc cgcctttatt gacgaatgcc
    35821 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    35881 gctttaaagc ggttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    35941 tattgatgga atacggcacg ggcgcagtta tggcagttcc gggtcacgac cagcgcgact
    36001 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggca gctgacggct
    36061 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    36121 agtttaatgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    36181 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc
    36241 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc
    36301 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    36361 cgattaaagc agatccggaa tgggcaaaaa ctaccgttaa cggtatgcca gcactgcgtg
    36421 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    36481 cggagtacaa agaaggtatg ctggattcca aagcggctaa ctactggctg ccggtggata
    36541 tttacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    36601 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    36661 agggtatggt gctggcagat gccttctact atgttggtga aaacggcgaa cgtaactggg
    36721 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    36781 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    36841 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    36901 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggc gtggaagggg
    36961 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    37021 ttgcggcact gaacgtcgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    37081 ataaaactat cgctaaagta accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    37141 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc cccgaccgat ggcgagcagg
    37201 atcgcgccct gatgcaggaa gcgctgctgg cggtggtccg tatgcttaac ccgttcaccc
    37261 cgcacatctg cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc
    37321 cgtggccgat tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    37381 ttaacggtaa agtccgtgca aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc
    37441 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    37501 aagtgattta cgtacccggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    37561 cgtgcgatat ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    37621 ctggcatctg cgtgatacca cgcaggttcc ttccactatg aaggtcatga tccaggactc
    37681 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    37741 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    37801 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    37861 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    37921 caaagtcttc cgttcgttct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    37981 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    38041 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    38101 tctggcaacg cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg
    38161 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    38221 gatcctctgt tattgcagga aagccaggac gctgttcgtc aggtcgctgc ggcacaagga
    38281 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    38341 ttatgccagg ctatgagtct gtttgccagt agacaaacgc tattgctgtt gttaccagaa
    38401 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctccatgac
    38461 gacctgctgt tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    38521 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    38581 cttccccgct gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgctgca
    38641 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggccca ggcactggag
    38701 cgtttatcgc tactctggcc ggacggcaaa ctgacattac cgcgcgttga acaggcggtg
    38761 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    38821 aagcgcgcgt tgcatattct tcagcagctg cgtctggaag gcagcgaacc ggttattttg
    38881 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    38941 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgcag gggcatgatg
    39001 agcgaggcgt taaatcgctt aagccagccg cagttacgtc aggccgtgca actcctgaca
    39061 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    39121 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    39181 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atcttaaacc
    39241 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    39301 tcctccgcat cgtccccagc cggaagcgaa cagcgcgcag cgtaaacaca tgcttgaact
    39361 ggcgatagct gacaagccac tgtttaccct tgatgaacgc gagctaaagc gcaatgcccc
    39421 ctcttacact gcgcaaacac tgaaagagtg gcggcaggag caaggaccgg acgtgccgct
    39481 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    39541 gatactcgac aatgcacatt tgatcgtctg tcggcgtcct ggttacccac ttgaaatggc
    39601 gcaaccgcaa taccagcaat ggctggagga tcatttgaca cataacccgg aagatcttca
    39661 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    39721 catcatccgc gaacgtttgc aaaatggtga atcgtgtgag gatttattac cggaaccggt
    39781 actgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    39841 atgcgactgt ggttaattcg tcatggtgaa acgcaagcga atgtcgatgg tctgtacagc
    39901 ggtcatgcgc ccacacccct gaccgcgcgc ggtattgagc aagcgcaaaa tctgcatacg
    39961 ctgctacatg atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat
    40021 accgcgcgac tggttcttag tgaccgccag caccccgttc atatcatccc tgaactcaat
    40081 gaaatgtttt ttggcgactg ggagatgcga catcatcgtg acctcatgca agaagatgcc
    40141 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    40201 tttcaggcat tttcgcaacg tgtggaacga tttatcgcaa ggcttagtga atatcagcac
    40261 tatcagaata ttttagtcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    40321 attggcatgc ctgccgagtc tatgtggcat ttccgcgttg accaggggtg ttggagcgcc
    40381 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    40441 gagaatgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    40501 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    40561 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    40621 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    40681 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    40741 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa
    40801 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgcc atgttatgtc
    40861 cattgctgat cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    40921 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    40981 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    41041 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    41101 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg
    41161 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    41221 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    41281 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg
    41341 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc
    41401 ggcacttacc cttccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    41461 gtggagcatc accaccaacc atccttatca ccgtgagtga taagtgagct ttgagtagaa
    41521 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    41581 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    41641 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    41701 ccgcattaag ctggtgccta tcgcacccag ccgcggcatt atctacgacc gtaacggtat
    41761 ccctctggct ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    41821 cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat ctgaccgatg atgatattgc
    41881 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    41941 cctgaccgaa gtacaagtag ctcgctttgc cgtcaaccag taccgttttc cgggtgttga
    42001 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    42061 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    42121 caactatgcg gcaacgcatg atatcggtaa gctgggcatt gaacgttact atgaagacgt
    42181 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    42241 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    42301 actccagcaa tatattgaaa cgttgctggc gggtagccgc gcagctgtgg tagtcaccga
    42361 tccgcgtaca ggtggggtgt tggcgctggt ttccacgcct agttatgacc caaacctgtt
    42421 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    42481 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgttgc
    42541 ggtttcggca ttgagtgccg gggtgatcac gcgcaatacg acgctgtttg accccggctg
    42601 gtggcaactg ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    42661 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    42721 cgatatgggg atcgatcgcc tctccgaatg gatggggaaa ttcggttatg gtcattacac
    42781 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    42841 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta
    42901 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    42961 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    43021 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcaaaaga
    43081 cggtatgtac ggtgttgcta accgccctaa cggtacggcg cataaatact ttgccagcgc
    43141 accgtacaaa attgcggcga aatctggtac agctcaggtc ttcggtctga aagcgaacga
    43201 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    43261 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    43321 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    43381 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    43441 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    43501 atcttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    43561 attggcatga tggagcgtaa aatcggccag attgcgatgg gtctggtcat catggtggtg
    43621 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    43681 attattttgc tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg
    43741 ctggacctcg gtattgttcg ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg
    43801 atggttgcac gctttatcaa ccgcgacgtt tgcccacctt cattgaagaa tacagctatc
    43861 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cttgggaaca
    43921 tcaatcctcg ttgcgctttc cggcctgttt gtactgttcc tctctggcct tagctggcgt
    43981 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    44041 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    44101 ggcgcaggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    44161 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccataccgac
    44221 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct
    44281 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    44341 ggtcgcgtca tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    44401 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    44461 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    44521 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    44581 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    44641 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcagaccc
    44701 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    44761 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    44821 cgaacccggc agtaacctga cggcctctgg cgaagctttc gatccgacac agttgacggc
    44881 ggcccatcca acgcttccaa tccccagcta cgccagaatc actaacctgg ctaacgggcg
    44941 aatgatcgtg gtgcgcatta atgatcgcgg cccttatggc aacgaccgcg tgatttcgct
    45001 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    45061 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    45121 caaacagact tacgccctgc cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    45181 agtgcctgtc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    45241 tccgaccggc gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc
    45301 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagctccgtc
    45361 gacaacgcct gcaacctcac ctgcaatggt gataccgcaa gccgcctcgc aaagcgccag
    45421 cggcaacttt atggtgcaag tcggggccgt tagcgatcag gctcgtgcgc aacagtacca
    45481 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    45541 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    45601 gcaaaccgaa gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat
    45661 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    45721 cggtagtgca tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc
    45781 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    45841 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa accatgatcc
    45901 cgggtgtacc gcagatcgac gcggagtcct acatcctgat tgactacaac tccggcaaag
    45961 tactcgccga acagaacgct gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    46021 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    46081 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    46141 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    46201 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    46261 ttggcttgat gaacagctac gttaacgccc tgggcctgaa aaatacccac ttccagacgg
    46321 tacacggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    46381 aggcgttgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    46441 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    46501 acggcatcaa aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg
    46561 aaggccagat gcgcttgatc tctgcggtga tgggcggacg tacttttaaa ggccgtgaag
    46621 ccgaaagtaa aaaattgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    46681 aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt
    46741 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    46801 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    46861 gaactatcaa cttccagctt gatggcaaaa cgatcgaaca acgcccgctg gttgtgctgc
    46921 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    46981 atcactggtt tggttaaaaa ttgagcactt gaaagtgtaa tttccgtccc catatactaa
    47041 gcatcagtaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    47101 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    47161 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    47221 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    47281 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    47341 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    47401 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    47461 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    47521 gccaatctcc caggctatgc atgaattcac cgatacccgc gatgatagta cccttgatga
    47581 aatctggctg gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca
    47641 tattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    47701 tcacgggccg gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    47761 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    47821 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    47881 ctcactgggt ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    47941 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgcaggaa tggaaatggc
    48001 taaaatatca caatggaaac ccgaagcgac gactaataat attgcaccac gtttactgga
    48061 aaatatttta gcgctactaa acaatcctga cttcgaatat attaccgctt aattccatac
    48121 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    48181 cttcgcgttt gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt
    48241 agctcaccca attggttaac tcattgaaat aattaccatg gtgagcaaaa gcgttccgtc
    48301 ccaattagac ataagattat tgtaattttt ttaaatatta ttttttcatg aatgaaattg
    48361 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa aatcgtcaga
    48421 aggtaaacca caaatattta caaccttacg aaatattgat cttacccttc tgactatttt
    48481 gaagttgcat atgtccataa aggaatcgtt aatgcagcga aagtgcttaa tctgcccccc
    48541 ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccaca
    48601 gcttttactg cgcaacccgc ccatcagcgg cgcggaaaac caactcagtc agtttcaaac
    48661 cgatctcatc accgataaca tgttttgcac aggaccatag atgttatccg taatgcattt
    48721 tgaaaaagta ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca
    48781 acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg
    48841 ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttt
    48901 cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg
    48961 tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tggcaacaga
    49021 gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac
    49081 acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga
    49141 agcctcctgc cctaacctgg cggaatgctt taaccacggc acagcaacgt ttatgatcct
    49201 cggcgctatt tgtacccgcc gttgcccgtt ctgtgacgtt gcccacggtc gcccggtagc
    49261 tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg cgctgcgtta
    49321 tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc
    49381 ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc
    49441 ggatttccgt ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt
    49501 gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga
    49561 ttacaactgg tcgcttaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac
    49621 caagtctggt ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg
    49681 cgacctgcgc cgtcatggtg tgacgatgct aacgctgggg caatatttgc agccaagccg
    49741 ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga
    49801 agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca
    49861 cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt acttacatct
    49921 gtctgataaa aaacccgctt tttgaagcgg gtttttgtat cgaacaagat attgagggag
    49981 cgtcctgctc gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg
    50041 cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg
    50101 ctccaaggtc tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg
    50161 ccgcaactac cagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata
    50221 accggcaaca gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac
    50281 acaatagcaa tctgtaacaa ggtgaatcaa gcaccgagta aaaaaacatc ataataattg
    50341 cggcggcgca aagcgacggt tgtttaagac gggcagttgc gcgcgtacct gacgcacacg
    50401 ttcgggcgtc acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac
    50461 gccaaaggga tctataatcc ggctttgccc gatatttttg ttcccgcact cccccgccgc
    50521 caccatataa caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc
    50581 tttgagcggc ccacgacccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc
    50641 ctgtgccagc gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat
    50701 cccttccacc tccagtaacg gagctatttc attaccagca tcaacatggc gtgattcctg
    50761 aatggcaaat gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg
    50821 tagcgccacc agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat
    50881 tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag
    50941 cagctgtgct gatttaaccg atagatctgc atcatgatca tcacgcgcca gtaatgcttc
    51001 cggcaggaca aacagcgaaa cgtcgttttc cgccgcctgc gccatcaacg agccacaaat
    51061 ctcagcgttc ttttcccaca cagatgtaac agcaaactgt cctgcggcaa ccagcattgc
    51121 cacctcctcc tgtcagagtc gtaaaattcg ccaacggcga ttattcggcg ttacacttat
    51181 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    51241 taggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
    51301 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    51361 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
    51421 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    51481 tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaat cttttggggt
    51541 cttttgccat gaccgcactg gctttctggc tgttttcggc ctcaaccgca cactaaaaac
    51601 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    51661 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    51721 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    51781 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    51841 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    51901 cggttaccag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    51961 aaaagtcgcg ttatttttga tgttaattat tttactggtt gattttaaag gatttactaa
    52021 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    52081 aggtacccgg aatgtaggtc atctgaaacg tcgcggggcc ataccccaca gaggccaatg
    52141 gcagtagaac cggaagaggg atgtaattcc agttatcgcg cgccgttacg ccagcggtaa
    52201 atcccaggcc taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt
    52261 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    52321 gccagtttcc tttttcatcc caacgcgact gaccaaaacc gccgccccac ggtcgctcgt
    52381 tatagcgatc ggttttttct ttgtcgtaag caaaacgtgc atgccaggtg atggcaggaa
    52441 tatataaatc ataatgttct ggctgttgcc aggtttgtgc aatattttct ctaaacgttg
    52501 tcatccactc atcagcgtta gcaaaaactt taccaacact gattaactga ataaaaacaa
    52561 agaaaaatat agcgacatat ttactcacgt tcatttgtga ccattaaaca tttatcaaaa
    52621 atctactact aacatagcaa agctacaatt aacataacct caatagcccc aacatgaaga
    52681 ataatatgaa tagctgccct atttatttcc gctagttcaa gataaatatt tccgcgcatc
    52741 gattaaagac gacgcatcgt tttctgcgat gggaatagtc ataaaagaaa aaccaagtcg
    52801 tt
//
LOCUS       DAZFRE010000025        50061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFRE010000025 DAZFRE010000000
VERSION     DAZFRE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.025"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(117..821)
                     /gene="ybhL"
                     /locus_tag="ACHKUI_001740"
     CDS             complement(117..821)
                     /gene="ybhL"
                     /locus_tag="ACHKUI_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6597948.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(958..1410)
                     /gene="moaE"
                     /locus_tag="ACHKUI_001741"
     CDS             complement(958..1410)
                     /gene="moaE"
                     /locus_tag="ACHKUI_001741"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6597949.1"
                     /translation="MAETKIVVGPQAFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1412..1657)
                     /gene="moaD"
                     /locus_tag="ACHKUI_001742"
     CDS             complement(1412..1657)
                     /gene="moaD"
                     /locus_tag="ACHKUI_001742"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6597950.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1650..2135)
                     /gene="moaC"
                     /locus_tag="ACHKUI_001743"
     CDS             complement(1650..2135)
                     /gene="moaC"
                     /locus_tag="ACHKUI_001743"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6597951.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2138..2650)
                     /gene="moaB"
                     /locus_tag="ACHKUI_001744"
     CDS             complement(2138..2650)
                     /gene="moaB"
                     /locus_tag="ACHKUI_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6597952.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2672..3661)
                     /gene="moaA"
                     /locus_tag="ACHKUI_001745"
     CDS             complement(2672..3661)
                     /gene="moaA"
                     /locus_tag="ACHKUI_001745"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6597953.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3649..3793)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4058..4966
                     /gene="yvcK"
                     /locus_tag="ACHKUI_001746"
     CDS             4058..4966
                     /gene="yvcK"
                     /locus_tag="ACHKUI_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6597954.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKEDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5004..7025)
                     /gene="uvrB"
                     /locus_tag="ACHKUI_001747"
     CDS             complement(5004..7025)
                     /gene="uvrB"
                     /locus_tag="ACHKUI_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6597955.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGNFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7603..8280)
                     /gene="bioD"
                     /locus_tag="ACHKUI_001748"
     CDS             complement(7603..8280)
                     /gene="bioD"
                     /locus_tag="ACHKUI_001748"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6597956.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8273..9028)
                     /gene="bioC"
                     /locus_tag="ACHKUI_001749"
     CDS             complement(8273..9028)
                     /gene="bioC"
                     /locus_tag="ACHKUI_001749"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6597957.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHIHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9015..10169)
                     /gene="bioF"
                     /locus_tag="ACHKUI_001750"
     CDS             complement(9015..10169)
                     /gene="bioF"
                     /locus_tag="ACHKUI_001750"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6597958.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCYDTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10166..11206)
                     /gene="bioB"
                     /locus_tag="ACHKUI_001751"
     CDS             complement(10166..11206)
                     /gene="bioB"
                     /locus_tag="ACHKUI_001751"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6597959.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11293..12582
                     /gene="bioA"
                     /locus_tag="ACHKUI_001752"
     CDS             11293..12582
                     /gene="bioA"
                     /locus_tag="ACHKUI_001752"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6597960.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKDEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            complement(12693..13823)
                     /locus_tag="ACHKUI_001753"
     CDS             complement(12693..13823)
                     /locus_tag="ACHKUI_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6597961.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            13932..14408
                     /gene="ybhB"
                     /locus_tag="ACHKUI_001754"
     CDS             13932..14408
                     /gene="ybhB"
                     /locus_tag="ACHKUI_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6597962.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14560..15843
                     /gene="ybhC"
                     /locus_tag="ACHKUI_001755"
     CDS             14560..15843
                     /gene="ybhC"
                     /locus_tag="ACHKUI_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6597963.1"
                     /translation="MNTFSVSRLALTLAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15984..18245)
                     /gene="ybhJ"
                     /locus_tag="ACHKUI_001756"
     CDS             complement(15984..18245)
                     /gene="ybhJ"
                     /locus_tag="ACHKUI_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6597964.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGKMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELLKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(18428..19861)
                     /gene="ybhI"
                     /locus_tag="ACHKUI_001757"
     CDS             complement(18428..19861)
                     /gene="ybhI"
                     /locus_tag="ACHKUI_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6597965.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(19937..20989)
                     /gene="ybhH"
                     /locus_tag="ACHKUI_001758"
     CDS             complement(19937..20989)
                     /gene="ybhH"
                     /locus_tag="ACHKUI_001758"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6597966.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTSNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            21173..22126
                     /gene="ybhD"
                     /locus_tag="ACHKUI_001759"
     CDS             21173..22126
                     /gene="ybhD"
                     /locus_tag="ACHKUI_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6597967.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(22167..23162)
                     /gene="pgl"
                     /locus_tag="ACHKUI_001760"
     CDS             complement(22167..23162)
                     /gene="pgl"
                     /locus_tag="ACHKUI_001760"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6597968.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            23317..24135
                     /gene="ybhA"
                     /locus_tag="ACHKUI_001761"
     CDS             23317..24135
                     /gene="ybhA"
                     /locus_tag="ACHKUI_001761"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6597969.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(24136..25194)
                     /gene="modC"
                     /locus_tag="ACHKUI_001762"
     CDS             complement(24136..25194)
                     /gene="modC"
                     /locus_tag="ACHKUI_001762"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6597970.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25197..25886)
                     /gene="modB"
                     /locus_tag="ACHKUI_001763"
     CDS             complement(25197..25886)
                     /gene="modB"
                     /locus_tag="ACHKUI_001763"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6597971.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(25886..26659)
                     /gene="modA"
                     /locus_tag="ACHKUI_001764"
     CDS             complement(25886..26659)
                     /gene="modA"
                     /locus_tag="ACHKUI_001764"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6597972.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(26826..26975)
                     /gene="acrZ"
                     /locus_tag="ACHKUI_001765"
     CDS             complement(26826..26975)
                     /gene="acrZ"
                     /locus_tag="ACHKUI_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6597973.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            27104..27892
                     /gene="modE"
                     /locus_tag="ACHKUI_001766"
     CDS             27104..27892
                     /gene="modE"
                     /locus_tag="ACHKUI_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6597974.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALLLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPINEATSLQQGQNVTAYFNADSVIIATLC"
     gene            27960..29432
                     /gene="modF"
                     /locus_tag="ACHKUI_001767"
     CDS             27960..29432
                     /gene="modF"
                     /locus_tag="ACHKUI_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6597975.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASHQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDVLGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GEMQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            29694..30710
                     /gene="galE"
                     /locus_tag="ACHKUI_001768"
     CDS             29694..30710
                     /gene="galE"
                     /locus_tag="ACHKUI_001768"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6597976.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            30720..31766
                     /gene="galT"
                     /locus_tag="ACHKUI_001769"
     CDS             30720..31766
                     /gene="galT"
                     /locus_tag="ACHKUI_001769"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6597977.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            31770..32918
                     /gene="galK"
                     /locus_tag="ACHKUI_001770"
     CDS             31770..32918
                     /gene="galK"
                     /locus_tag="ACHKUI_001770"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6597978.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            32912..33952
                     /gene="galM"
                     /locus_tag="ACHKUI_001771"
     CDS             32912..33952
                     /gene="galM"
                     /locus_tag="ACHKUI_001771"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6597979.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            34155..34907
                     /gene="gpmA"
                     /locus_tag="ACHKUI_001772"
     CDS             34155..34907
                     /gene="gpmA"
                     /locus_tag="ACHKUI_001772"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6597980.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(35074..36126)
                     /gene="aroG"
                     /locus_tag="ACHKUI_001773"
     CDS             complement(35074..36126)
                     /gene="aroG"
                     /locus_tag="ACHKUI_001773"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6597981.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            36442..36822
                     /gene="ybgS"
                     /locus_tag="ACHKUI_001774"
     CDS             36442..36822
                     /gene="ybgS"
                     /locus_tag="ACHKUI_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6597982.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNIDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            36936..37877
                     /gene="zitB"
                     /locus_tag="ACHKUI_001775"
     CDS             36936..37877
                     /gene="zitB"
                     /locus_tag="ACHKUI_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6597983.1"
                     /translation="MAHSHSHASSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(37874..38593)
                     /gene="pnuC"
                     /locus_tag="ACHKUI_001776"
     CDS             complement(37874..38593)
                     /gene="pnuC"
                     /locus_tag="ACHKUI_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6597984.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(38631..39674)
                     /gene="nadA"
                     /locus_tag="ACHKUI_001777"
     CDS             complement(38631..39674)
                     /gene="nadA"
                     /locus_tag="ACHKUI_001777"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6597985.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(39952..40027)
                     /locus_tag="ACHKUI_001778"
     tRNA            complement(39952..40027)
                     /locus_tag="ACHKUI_001778"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39992..39994),aa:Lys,seq:ttt)
     gene            complement(40061..40136)
                     /locus_tag="ACHKUI_001779"
     tRNA            complement(40061..40136)
                     /locus_tag="ACHKUI_001779"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40101..40103),aa:Lys,seq:ttt)
     gene            complement(40185..40260)
                     /locus_tag="ACHKUI_001780"
     tRNA            complement(40185..40260)
                     /locus_tag="ACHKUI_001780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40225..40227),aa:Lys,seq:ttt)
     gene            complement(40264..40339)
                     /locus_tag="ACHKUI_001781"
     tRNA            complement(40264..40339)
                     /locus_tag="ACHKUI_001781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40304..40306),aa:Val,seq:tac)
     gene            complement(40391..40466)
                     /locus_tag="ACHKUI_001782"
     tRNA            complement(40391..40466)
                     /locus_tag="ACHKUI_001782"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40431..40433),aa:Lys,seq:ttt)
     gene            complement(40469..40544)
                     /locus_tag="ACHKUI_001783"
     tRNA            complement(40469..40544)
                     /locus_tag="ACHKUI_001783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40509..40511),aa:Val,seq:tac)
     gene            complement(40580..40655)
                     /locus_tag="ACHKUI_001784"
     tRNA            complement(40580..40655)
                     /locus_tag="ACHKUI_001784"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40620..40622),aa:Lys,seq:ttt)
     gene            complement(40820..41611)
                     /gene="cpoB"
                     /locus_tag="ACHKUI_001785"
     CDS             complement(40820..41611)
                     /gene="cpoB"
                     /locus_tag="ACHKUI_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6597986.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGAANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(41621..42142)
                     /gene="pal"
                     /locus_tag="ACHKUI_001786"
     CDS             complement(41621..42142)
                     /gene="pal"
                     /locus_tag="ACHKUI_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6597987.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(42177..43469)
                     /gene="tolB"
                     /locus_tag="ACHKUI_001787"
     CDS             complement(42177..43469)
                     /gene="tolB"
                     /locus_tag="ACHKUI_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6597988.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(43602..44867)
                     /gene="tolA"
                     /locus_tag="ACHKUI_001788"
     CDS             complement(43602..44867)
                     /gene="tolA"
                     /locus_tag="ACHKUI_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6597989.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44932..45360)
                     /gene="tolR"
                     /locus_tag="ACHKUI_001789"
     CDS             complement(44932..45360)
                     /gene="tolR"
                     /locus_tag="ACHKUI_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6597990.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(45364..46056)
                     /gene="tolQ"
                     /locus_tag="ACHKUI_001790"
     CDS             complement(45364..46056)
                     /gene="tolQ"
                     /locus_tag="ACHKUI_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6597991.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(46053..46457)
                     /gene="ybgC"
                     /locus_tag="ACHKUI_001791"
     CDS             complement(46053..46457)
                     /gene="ybgC"
                     /locus_tag="ACHKUI_001791"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6597992.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(46607..46900)
                     /gene="ybgE"
                     /locus_tag="ACHKUI_001792"
     CDS             complement(46607..46900)
                     /gene="ybgE"
                     /locus_tag="ACHKUI_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6597993.1"
                     /translation="MSKIIATLYAVMYKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46900..47013)
                     /gene="cydX"
                     /locus_tag="ACHKUI_001793"
     CDS             complement(46900..47013)
                     /gene="cydX"
                     /locus_tag="ACHKUI_001793"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6597994.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(47028..48167)
                     /gene="cydB"
                     /locus_tag="ACHKUI_001794"
     CDS             complement(47028..48167)
                     /gene="cydB"
                     /locus_tag="ACHKUI_001794"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6597995.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(48183..49751)
                     /gene="cydA"
                     /locus_tag="ACHKUI_001795"
     CDS             complement(48183..49751)
                     /gene="cydA"
                     /locus_tag="ACHKUI_001795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6597996.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        12311 a        13334 c        12390 g        12026 t
ORIGIN      
        1 cagaaaacgc ttccgctcgt ccataaggca tattttatac ctggtacttg ttaagtgtcg
       61 tttttaataa gaaaaaaatc ccgacacatg gccgggatat tgatgatgaa aaagaattaa
      121 cggcggttgc cgaagatccg cagcaacatc aggaacaggt tgatgaagtc cagatacaag
      181 gttaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgagt atcaatctgc
      241 tcacccatat ttttcagttt ctgggtgtca tacgccgtca atccgacaaa gacaatcacg
      301 ccgatgtagg tgactgccca catcaatgct tcgcttttca gccagaagtt aaccagcgat
      361 gccagcacaa tgccgattaa cgccataaac agcatattgc caaagccgct tagatcgcgc
      421 ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta
      481 ctggcgatag aagcagcggt atagacaatg aaaatactgg aaagcgtaag acccgtcagc
      541 gccgaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct
      601 gataacacaa taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta
      661 gtaaacaaca gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgctgtc
      721 agcaacaagc caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc
      781 cggggttgta cgattgaatc agaacgtggg aatctgtcca tgactttctc ctgaagatat
      841 aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgt
      901 atgtactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa caataaacta
      961 ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg acccagcggt cgccttccgg
     1021 cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc
     1081 ggcttcaaac gcactgctgc gatgcgcact ggtaacaccg acaaaaacaa tttcatcgcc
     1141 cggccataat tcaccaatgc ggtgaattac cgtgacgcgc cccagcggcc agcggttacg
     1201 cgcttcatca acgatttctg ccagtgcttt ttctgtcatt cccggatagt gttcgagggt
     1261 taatgctttg acgctgtcgc ccaggttatg gttgcgcacc ttaccggtaa aggtgactac
     1321 cgcaccgtct tcgtcacgct cagccagcca cgggtactct tctcctacgc tgaacgcctg
     1381 cgggccgaca acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag
     1441 ccacttcgtc gccgtcagtc agcgaatggt caaaactcac cagcgtctgg ttgacagcag
     1501 ccagcaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccagatgtt
     1561 ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc ggttgcatct gttcccacca
     1621 actcgcgcac ctgggcgaaa aaaagaacgt taatcatccg cttccacctt aaagtcaccc
     1681 gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc
     1741 gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa cgcttccatt
     1801 tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgtacccg attgtgctcc
     1861 ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcgggtgaca cagcgggatc
     1921 agatcccagg tgcgttttgc cgcctgaata ccggcgatac gcgcagtggc aaatacgtcg
     1981 cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag
     2041 gcttcggcgc gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct
     2101 tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga
     2161 aattacacgg acgcgtacgg gcatccagct gtggcgcaat gatattttcc catgcggtac
     2221 ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg acgcccgcta
     2281 ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcgaa cgacaacatc cggaacactt
     2341 caccaaaacc ttcaacttca cggtcgaaca acggcagcag cgcttcggga gcctgatcgc
     2401 cttccgtcag gccagtacca cccgtaatca acaccacttg tacatcatcg ctggcgatcc
     2461 acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa
     2521 cgtgatggcc cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt
     2581 caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta
     2641 cctgactcat ctgatctctc cttttgacgt tttagccacc aatgtacgat aagttttgcg
     2701 taataccggt gttgttttga tgcaggaaat gggtctgttt tttctcccgc agcgccgctg
     2761 aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa
     2821 cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt
     2881 tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat
     2941 ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc
     3001 cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct
     3061 cgctgccctc gcccgtttcc atcagttcga tgaaacgcag ctggatggga cgatgctgga
     3121 tccagttcag aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga
     3181 ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat tcctgccatg acctggttga
     3241 atttatcctg cccggtaatg gcatgaaact ggcgagcgtc cagactgtcg acgctgacgt
     3301 tgatgccagt aagtccagca tcgcgccagt tcgccacatc gcgctccaga cggtaaccat
     3361 tggtggtgac cgctatctgg cgaatagcgt cgttttctcg cacagcggcg atgatatcgg
     3421 taaagtcgcg gcgtaaagat ggctcaccac ctgtcagacg gactttttcg gtgcccaaac
     3481 tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt cagaaagcct ttattggtga
     3541 cgccgctcgg cttgtaacca tccggcaggc agtaggtgca acgaaagtta cacacatcag
     3601 taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca
     3661 tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct
     3721 ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt
     3781 gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct
     3841 atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg
     3901 cggttttact ttgatataca tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag
     3961 aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcctc gcaatttgcg
     4021 ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga
     4081 tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact
     4141 ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc accgataacg gtggctcgac
     4201 ggggcgtatt cgccgctcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa
     4261 tcagctgata actgaaccca gcgtcgcttc cgcaatgttt gaatatcgtt tcggcggtaa
     4321 tggcgaactt tccgggcaca accttggaaa cctgatgtta aaggcactgg atcaccttag
     4381 cgtgcgacct cttgaagcga tcaatctgat tcgaaatctg ctgaaagtgg acgcacattt
     4441 gattccgatg tcggagcatc ctgttgatct gatggcgatt gacgatcagg ggcatgaagt
     4501 atacggcgag gtcaatatcg accagttaac tgcgccgatt caagagttat tgttaacgcc
     4561 gaatgtaccc gcaacgcgtg aggcggttca cgctatcagt gaagcggatc tcatcattat
     4621 tgggcctggc agtttttaca ccagcctgat gccaattctg ctgctgaagg aaatcgcaca
     4681 ggcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc gtgagttgag
     4741 tttacctgcg gctaatctga agctggaaag caagctggca attatggagc agtatgttgg
     4801 taaaaaggta attgatgcgg tcatcgtcgg gccaaaagag gatgtctcgg cggtgaaaga
     4861 gcggattgtg attcaggaag tgctggaggc cagcgatatt ccgtatcgtc atgaccgcca
     4921 gttgttgcat agcgcgctgg aaaaggcatt acaggcttta ggttaacacg aagccggaag
     4981 aatattccgg cttcgtcatc ttgtcacgac gcggcaataa acagctcacg caactgatgc
     5041 aactggtcac gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc
     5101 aacccttcca gttcatggat cttctgctgt aacgctttag gcgacatatc catcggcaca
     5161 ttatccggtt caacaatcgg gcgcgatttt cctctgccct tcgctttggt tttggcaatg
     5221 ttctgcccca gcgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg
     5281 tgttcttcgt tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc
     5341 gccattgatg gggtgatctt atcgccatag agaatcgctt taccgttaac gttacgtgcc
     5401 gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg
     5461 tcgaggatcg ccaccagcga aacttccggc atatccagac cttcgcgcag taagttgatc
     5521 cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat acgttcgacg
     5581 gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag atattcagtg
     5641 agatcttccg ccatccgctt ggtcagagtt gtaaccagta cgcgttcgtt aattgccgct
     5701 cgctgacgaa tctccgatag aagatcatcc acctgtgtcg ctaccggccg cacttcgata
     5761 atcgggtcga gtaagcctgt tggacgcacc acctgatcca ccacatcgcc accggatttt
     5821 tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg
     5881 aactcttcaa atttcagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc
     5941 accagtgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg tggaatggtg
     6001 acgtgagatt catcgaccac cagcagccca tcagcaggca ggtaatcaaa cagcgtcggc
     6061 ggtggctcac ccggtccacg accggagagg aagcgcgagt agttctcaat ccccgaacag
     6121 tagcctagct cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct
     6181 tccagcagtt tgttgttttc caatagcact ttacgtctgg cggccagctc ttctttgatc
     6241 tcctccatcg cctggacgat gcgctcgcgt ggcgtgacgt agtgcgtttt cgggtagatg
     6301 gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt
     6361 tccacttcct cgtcaaacag ctccacgcga agtgcaatgt catccgattc tgccgggaag
     6421 atatcaatca cctcgccacg aacacggaaa ttaccacgct ggaatgcttg atcattacga
     6481 gcatattgca gctccgccag tcggcgcagg atcgcgcgct gatcgataat cataccgacc
     6541 gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa
     6601 acagacgcca ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggacaaacgc
     6661 atctgttcga tatgttcgtt taccgaggca tctttctcaa tgaaggtgtc ggaactcggt
     6721 acataggcct ccggctgata gtagtcgtag taggagacga aatattccac cgcgttttcc
     6781 ggaaagaatt ctttcatttc gccatacagc tgggccgcca gcgttttgtt gggcgcaagt
     6841 accatggttg ggcgctgaag gtcagcaatg acattggcaa tggtgaaggt tttcccggag
     6901 ccagtcacgc caagtaacgt ctggtgcgcc agaccatctt ccagcccctc ttcgagacgt
     6961 cgaatcgcct ccggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta
     7021 ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact
     7081 taattatgcc aacaaattat actggataaa aaaacagttc atcaccataa tatttctgtt
     7141 acagcgtaaa ctccgctgtc aatcatgaac aagatttact ctgcggcgag ataaaactcc
     7201 ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgattgatg
     7261 acagttttat ggcaagagat gcctgttcag tgactgccat tgcaattatc taacctgtta
     7321 aaaaataaaa gatatttctt tgagccgtct ttaacgccaa tttgcgtgca agccgcgtat
     7381 tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca ggaatagtgg
     7441 ataactgtct gcagccccta tcccccgccg cctgggcaat tcccacattc actgcatgat
     7501 gcggaggtaa aatttttttc gtgatttttt agaatttaat tggttaaatt gcagtcaatc
     7561 ggagacgcga tctcgctcac aatttaacca agcacaggat gactacatta aggcaagatt
     7621 gatgtacttt ccggttgccg cattttctgg attttctgca agccagggaa tttcgcccag
     7681 cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat tcagcgtgac gttttcccgg
     7741 aggcgtgaca tcgttcgcca cccaccccgc cagagtcagt ccggcgtgta gtattgcctg
     7801 cgcagtcaac atcgcgtgat taatacagcc gagtttcacg cccacaacca gtatcactgg
     7861 cagttgttcc tgtgttaccc aatccgcaaa agtgaaagtg tcagaaagcg gcgtaaacca
     7921 gccgccagca ccttccacta acacccagtc agcctgttgt tcaagcgcgc gtaatccggc
     7981 gctcattacc aatgcttcta tcggcctgcc ctcttgcgcg ctgatgatat gcggcgaggt
     8041 gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc agctgcaggc tactgttgcg
     8101 ctgtaacgcc agcgcgtcac tattacgtaa gcctttcggg gtcttttcgc tgccagaggc
     8161 caccggtttg taacctgccg tccggtagcc tgctgccttt gcggcttgta aaagcgcgca
     8221 gctggcgaca gttttcccca cttcggtatc cgttccggtg acaaaataac atttactcac
     8281 gagcaatcac tcccaaaaaa agatgatacg tcagaggata tcgcccctgt tgttgcggcc
     8341 aggccagttg caatcgctgc aactgcgaac gcgttaatat tcgcgggtca cgcccttcat
     8401 gaatatgcgt ggcaccgatg cctttcagcg aacgcatggc actgagcgca tcatcaaacc
     8461 acagcgtgat gggctgaata taatgttgat aatgaacggc gtttagcgac tgttcgattt
     8521 tatctggcgg taaaaagcga ttagcatgcg ggcgctcgtc caccgcctgc cacgcctgat
     8581 gcagttcggg taacgatccc tgcaccagcg tggtaaacgc gaccacgcct ccggggcgca
     8641 ccacccgata cagctcgcgg agtgccatgg ataaattacc gcaccactgc accgcgagat
     8701 tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag ggattcgata tctcccgcca
     8761 gataatggtc tgcggcatcc ttctggcgtg cctgaacaag cattggcgga gagagatcta
     8821 acgcagtcac ctgcgcgtga cgctctcgcc agcggcggct catccagcct ggtccacaac
     8881 ccgcatccag tacgcgggtg tatttacgct gtgggagcat tgccagtaag gcgtcagcac
     8941 tctggcgctg tagatcggcg tgttgctcat agtgtgcggc tgcccgtcca aatgccgcag
     9001 caatggcttg tttattaacc gttgccatgc agcacctcca gcagacggtc gatatcctgc
     9061 atttcatgcg ctgcggttag cgttaagcgc agtcgcgcag taccagcggg tacggttggt
     9121 gggcgaattg ccgtgaccca acagccttgc tggcgcagtt tttctgccag ttgtaacgca
     9181 cggctgttat caccgacgat caatggctgg atggcgctgc atgagtcagc aagcgtaaac
     9241 ggcaaatcct gcactccggc acgaaaacga gtaatgagtg ccgccagttt ttcgcgccgt
     9301 gcatccccct catcactgcg aatgaccgcc agcgacgcac gtaatgcctg cgcctgagcg
     9361 ggcggcatac tggtgctgta gataagatgg cgagcgaatt gcagcagata atccgccacg
     9421 gtatcgtagc aaagcaccgc tgccccgctg acgccaaatc ctttgccaaa cgtcaccacc
     9481 agcaattctg gttttacctt ttgcagccag cagctgccac gcccctgctc cccgataacg
     9541 cccgtgccgt gggcgtcatc gaccatcaac cagccatcgt gctgttgcgt tacttgctgg
     9601 atttcctcca gtggcgcact atcgccgtcc atgctgaaca caccttctgt cactaccagt
     9661 tgctgccccg gacaggggga agcaagcagt cgcgccagat gagtgacatc gttatgagca
     9721 aaacggcgaa gcggcgacgg gcttaaactt gcagcctcca gcaatgaggc atggctaagc
     9781 cggtcggcaa caatacggtc ctctttcgcc atcatcgcgg caatgacagc ctgattagcg
     9841 gcaaaaccag aaataaacag cagtgcccgc gaatagccca gccattcggc cagttcttct
     9901 tccagcacct ggtgcgccac gctataaccg ctgacgtgac cggaaccgcc gctaccgaca
     9961 ccaaattgtt ccgccccctg ctgccaggcg cggataattt gcggatgatg gcttaaacct
    10021 aaataatcgt tactggaaaa gttcagatac tggcaatcat ctgccaccag ccagcgtccg
    10081 gctccttgtg ccaccggata acggcgacgc aaggcatcag caacacgccg cgcatcaagc
    10141 gccgcgtcga ttttctcctg ccagatcata gtgctgccgc gttgtaatat tcgtcagtgt
    10201 ccggggtcat cagcgcctgc tccagacgct gctgttgttc gttatcgccc gccagcacgg
    10261 cagtttgctg cggatttagc cccagtttgc ggaacagttg caggtcttta tcttcttccg
    10321 gattcggcgt agtcagcagt ttgcaaccgt agaaaatcga gtttgcgcca gccataaagc
    10381 acatcgcctg agtctgttcg ttcatctgct cgcgtccggc agaaaggcgt acgtaagagg
    10441 tcggcatcat gatccgcgct acggcaatgg tacggataaa atcaaaggca tcgacatcgt
    10501 cgttatccgc cagcggcgtg cctttcacct tcaccagcat gttgattggc acgctttccg
    10561 gcggcgtcgg caagtttgcc agttgcagca ataatccggc gcgatctttc accgtttcgc
    10621 ctaagcccac aatgccacct gaacagactt taatcccagc ttctcgcact ttttccagcg
    10681 tatcaagacg ttcctgatag gtgcgggtgg tgatgatatt gccgtaaaac tccggcgagg
    10741 tgtcgaggtt gtggttatag taatccagcc cggcattcgc caggcgctgc gcttgagatt
    10801 cactcaacgt gcccagcgtc atacacgcct ccagacccat cgcttttacc ccctgcacca
    10861 tttgttccag gtacggcata tcgcgttcgt ggggattctt ccacgccgcg cccatacaga
    10921 aacgcgtcga tcccgccgct ttcgctttgc gcgccgactc cagcacctgt tcaacttcca
    10981 tcaaccgctc ggcttccagc ccggttttgt agcgcgagct ttgcgggcaa tatttgcaat
    11041 cttccggaca agctccggtc ttaatcgaca gcaaagtgct gacctgcacc tgacgaggat
    11101 cgaaatgctg acgatgcacc tgctgcgctt caaacagcag atccagcaac ggtttttcaa
    11161 ataattctgt gacttgcgac aatgtccagc gtgggcggtg agccatgggg cttctccaaa
    11221 acgtgttttt tgttgttaat tcggtgtaga ctcgtaaacc taaatctttt caatttggtt
    11281 tacaagtcga ttatgacaac ggacgatctt acctttgacc aacgccatat ctggcaccca
    11341 tacacatcca tgacctcccc gctgccggtt tatccggtgg caagcgccga aggttgcgag
    11401 ctaattttgt ctgacggcag acgcctggtt gacggtatgt cgtcctggtg ggcggcgatc
    11461 cacggttaca atcacccgca gcttaatgcg gcgatgaagt cgcaaattga tgctatgtcg
    11521 catgtgatgt ttggcggtat cacccatgcg cccgccattg agctgtgccg caaattggtg
    11581 gcaatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg actccggctc cgtagcggta
    11641 gaagtggcga tgaaaatggc gttgcagtac tggcaggcca aagacgaagc gcgccagcgt
    11701 tttctaacct tccgcaatgg ttatcatggc gatacctttg gcgcgatgtc ggtgtgcgat
    11761 ccggacaact caatgcacag cctgtggaaa ggctatctgc cagaaaacct gtttgctccc
    11821 gccccgcaaa gccgcatgga tggcgaatgg gatgagcgtg atatggtggg ctttgcccgc
    11881 ctgatggcag cgcatcgtca tgaaatcgcg gcggtgatca ttgaaccgat tgtccagggc
    11941 gcaggcggga tgcgcatgta ccatccggaa tggttaaaac gaatccgcaa aatgtgcgat
    12001 cgcgaaggta tcttgctgat tgccgacgag atcgccaccg gatttggtcg taccggcaaa
    12061 ctgtttgcct gtgaacatgc agaaatcgcg ccggatattt tgtgtctcgg taaagcctta
    12121 accggcggca caatgaccct ttccgccacg ctcaccacgc gcgaggttgc ggaaaccatc
    12181 agtaacggcg aagccggctg ctttatgcat gggccaactt ttatgggcaa tccgctggcc
    12241 tgcgcagcag caaacgccag cctggcgatt cttgaatctg gcgactggca gcatcaggtg
    12301 gcggctattg aagcgcagct acgcgagcaa cttgcccccg cctgtgacgc cgaaatggtt
    12361 gccgatgtgc gcgtactggg ggcaatcggc gtggtcgaaa ccactcgtcc ggtgaatatg
    12421 gcagcgctgc aaaaattctt tgtcgaacag ggtgtctgga tccggccttt tggcaaactg
    12481 atttacctga tgccgcccta tattattctc ccgcaacagt tgcagcgtct gaccgcagcg
    12541 gttaaccgtg cggtgcagga tgaaacattt ttttgccaat aacgggaagt cagcgtgagg
    12601 gtttctggct acactttctg caaacaagaa aggagggctc ttgacggctc cccgccctga
    12661 atgacgggga ttcccacttc aacaagccga acctaagccg cgtgctgcga ttcagggttt
    12721 acaggctctc cgtgggctaa cactgccagc ccggcagctt taatattacg tgccgcgtta
    12781 atgtcgcgat catggtctgc gccgcattca gggcagtgcc atttacgaac attaagaggc
    12841 attttttgca tggtgaaacc gcagcaacta cagcattttg aggacggtaa atactggtca
    12901 atggcgacca ctgaccgccc ggcccatttg cctttgtact ggagctggcg aacaagttcg
    12961 ctccagcctg cgtcagctat tgctttagac agcttcgggt tgcggatcat gtttttcacc
    13021 ttgagggatt cgacgcaaac aacttggttt tcgttaatca gtttgcggga caacttgtgc
    13081 agattgtcca tccggcaatc ggcgattttc gcgtggagtc gggcgacctt taagcgggct
    13141 ttaattcggt ttcttgagcc tttttgcttc ctgcttaaac gtcgctgtag cagcgttaat
    13201 cgcttcgcat atttagcggt gtggcgggga ttgtcggttt tgaatccggt atcggtgacg
    13261 aataaatctt ttaagcccac atcaatgccg accgttttag cggtaacagg cattgataca
    13321 ggttcaaact cacacaggca ggaaacaaag tacctgccag cgctatctct ggaaatggta
    13381 acggttgacg gcgcagatgg taattctcga ctccagcgaa catccagcgg cgacttgctc
    13441 tttgctatat acaactcgcc gtcacggtgt ttaaacgcgc tggcagtgaa ctcagccacc
    13501 tgtttgtgcc gtttgctttt gaaagccgga tatgcagctc gtccggcaaa gaagttagca
    13561 aaggcggctt gttggtggcg caacgactgc tggaggggaa cgcaggaaac atcattcagc
    13621 catatgtatt caggctcttt tttgagcgcc gtaaggcgag cgttggcctg tagataaccg
    13681 atcttttctt ttcgctcgta gtacgcatcg gtacgccaac gaaggatgga attgtagacg
    13741 aagcgcacac agccaaacgt ctgagctaaa agctcagcct gctcagttgt cgggtaaaac
    13801 cggtatttat atgcgcgttt catgtgttca catactaaag aggaaaatgt gattatgcaa
    13861 agtacagtta gtcggaaaac cgcctccttt cctccccggt ctgaaggccg aggtttcccg
    13921 gaggcattct aatgaaactc atcagtaacg atctgcgcga tggcgataag ctgccgcatc
    13981 gtcatgtctt taacggcatg ggttacgatg gcgataatat ttcaccgcat ctggcgtggg
    14041 atgatgttcc tgcgggaacg aaaagttttg ttgtcacctg ctatgacccg gatgcgccaa
    14101 ccggctccgg ctggtggcac tgggtagttg ttaatttacc cgctgatacc cgcgtattac
    14161 cgcaagggtt tggctctggt ctggtagcta tgccagacgg cgttttgcag acgcgtaccg
    14221 actttggtaa aaccgggtac gatggcgcag cgccgccgaa aggcgaaacc catcgctaca
    14281 tttttaccgt tcacgcgctg gatgtagaac gtattgatgt cgatgaaggt gccagcggcg
    14341 cgatggtcgg gtttaacgtt cattttcact ctctggcgag tgcctcgatt actgcgatgt
    14401 ttagttaatc actctgccag atggcgcaat gccatctggt atcacttaaa ggtattaaaa
    14461 acaacttttt gtctttttac cttcccgttt cgctcaagtt agtataaaaa agcaggcttc
    14521 aacggattca tttttctatt tcatagcccg gagcaacctg tgaacacatt ttcagtttcc
    14581 cgtctggcgc tgacattggc ttttggcgtg acgctgaccg cctgtagctc aacaccgccc
    14641 gatcaacgtc cttctgatca aaccgcgcct ggtacctctt ctcgcccgat tctgtcggca
    14701 aaagaagcgc agaatttcga tgctcaacac tattttgcat ccctgacacc aggtgcggca
    14761 gcgtggaatc cttccccgat taccctgcct gcgcaacctg actttgttgt cggcccggcg
    14821 ggtactcaag gtgtaacgca taccacgatt caggcggcgg tagatgcggc aattatcaag
    14881 cgtaccaaca agcgccagta tattgccgtg atgcctggtg agtatcaggg aacggtgtat
    14941 gtccctgccg ctccgggtgg aattactcta tacggtacag gtgaaaaacc gattgatgtg
    15001 aagattgggc tttcccttga tggtggcatg agccctgccg actggcgtca cgacgtcaac
    15061 ccgcgcggca aatatatgcc aggtaaaccg gcgtggtata tgtacgatag ctgccagagt
    15121 aaacgcagcg acagtatcgg tgttctctgc tctgcggtct tctggtcaca aaacaatggc
    15181 ctgcaactgc aaaacctgac catcgaaaac acgctgggcg atagcgtaga tgcgggtaac
    15241 catccggcgg tggcactgcg tactgatggc gacaaagtgc agatcaataa cgtcaacatt
    15301 ctcggtcgtc agaacacctt ctttgtcacc aacagcggtg tgcagaaccg tctggaaacg
    15361 aatcgtcagc cgcgtacgct ggtgaccaac agctatattg aaggggatgt ggatatcgtt
    15421 tctggtcgcg gcgcagtggt gttcgataac accgaattcc gcgtggtgaa ctcccgtacc
    15481 cagcaagaag cgtatgtgtt tgcaccggct acgctgtcca acatttacta cggtttcctc
    15541 gccgtaaaca gccgtttcaa tgcttccggt gatggcgtgg cgcaactggg ccgctcgctg
    15601 gatgttgatg ccaataccaa cggtcaggta gtgatccgtg atagcgccat caacgaaggt
    15661 tttaacacag ccaaaccgtg ggctgatgcg gtgatctcta atcgtccatt tgcgggtaac
    15721 accggcagcg ttgatgataa cgacgaagta cagcgcaatc tgaatgacac taactacaac
    15781 cgcatgtggg aatacaataa ccgcggcgtg ggtagcaaag tggttgcaga ggcgaagaag
    15841 taggagctgt taactatgtg ccggatgcgg cgtaaacgcc ttatccggcc tacagttcga
    15901 tacgatttgt cggtcggata agatgcgcca gcatcgcatc cgacaataag tgccggatgc
    15961 gacctacatt cacatggcgc tttttacatc tgacggtttt tattgaagtt aatcaaacta
    16021 cctgccttga taatctcgcg ctcttcagca gtcagacttt ccatatagag cgtaatctcc
    16081 gttaccggtg catcttcatg gatcacataa cctttaaacg tcgttccagg attatccagc
    16141 gccgctttaa tgccagggat ataaatgtaa tcacctacgt caaagttcgg tacttccgcc
    16201 atctgcagcg gtaacatccc ccagttgatg acgttagaac gatagcgttt agtcgcgtac
    16261 tcctcggcaa tattcgccag accgccaatc acacgctgac aactcgccgc ctgttcacgc
    16321 gcagaaccat cgcctggttt caccgcataa accatgctgc caatttcagt ttgcagcgga
    16381 tcaacatgtt cctgaccagc aatctgctta atgcgcgcaa acacatctgc catctcgctg
    16441 acatttcccg ccagacgctg attttccagc tcggcagtcg ctttacttct gccaacataa
    16501 cctggatcgc ggcgtgacag agtaaactcc gccagaccaa tcggatttga acgatatgaa
    16561 gaggtttcac cggaaggaat cagttcgtcg gtggtggtca cttcgtcgag gatcttcgag
    16621 cacactttga ggacgatatt gtcagtcagc gcacccaatt ctggccagtc tttaatgttc
    16681 ggtccgtaaa tcagcggttg ctgggtcgcc cctttcacaa agccctgata aacacggttt
    16741 ttatacggcg ttacatcgaa ggcgtactcc ggcacgttgt cccagcaatc gagttcgctg
    16801 gcagaggtta aatagccacc gtttgccgca gtcgcagcga tagaacgagc gtccatcaac
    16861 gccaccgctg acatctgccc attagctggc ttagagcctt cgcggttcgg gaagttacgc
    16921 gtggtgtggc gaatactcaa accgttgttg attggcgtat cgcccgcgcc aaagcatggg
    16981 ccgcagaacg cggttctgat gattgcgcct gcgccgatca aatctgctac cacacctttt
    17041 ttggcgagat ccataaacac cggctgtgat gacgggtaaa ccgctaacga gaaggtgtca
    17101 ttgccgcagg attgaccgcg cagcgcattc gccgccgcga tgacgttttc gtagttaccg
    17161 ccagaacagc ccgcgataat cccctgctgc actttcaggc gaccattttc cactttatcc
    17221 agcagcgaga gtttggcttt accgtgcgcc acgcgttcgg actcaatttc aatctcacgc
    17281 agaatgtcgg tcaggttctg gttcagcgtg tcgatttcat acacgttgct cgggtggaat
    17341 ggcagcgcaa tcattggttt gatggcgctt aaatcaacgc tgatgcagcc atcgtagtac
    17401 gccattggtt gagggttaag ttggcagtaa tcctggccgc gaccgtgcag cgccagccag
    17461 ttatggactt cttcatcggt ttgccagaca gaacttaaac aggtcgtttc agtggtcatc
    17521 acgtcaacgc tgttacggaa atcggtagag agcgctgcaa caccgggacc tacgaactcc
    17581 atcactttgt ttttgacgta accgtttttg aacacagcac cgatgatagc cagcgccaca
    17641 tcctgcggcc ccacatacgg cgcaggtttt ccggtcagat gcaccgcaac cacgcccgga
    17701 tagtcgatat cccaggtgtc attaagcagc tgttttaaca actcgccgcc gccctcgcca
    17761 accgccattg tccctaacgc accgtaacgg gtatggctgt ctgacccgag gatcattttg
    17821 ccgccgcccg ccatcatttc acgcatatat tgatggatga ccgcaatatg cggtggcaca
    17881 aaaataccgc cataacgctg ggccgccgat aaaccaaaaa catggtcatc accgttaatg
    17941 gtgccgccca cagcacagag tgagttatgg cagttggtca gcacatacgg cagcgggaaa
    18001 cgttccatac cggacgcttt agcggtctgt acaataccaa caaaggtaat atcgtgagag
    18061 gctaatgaat caaacttaat tttaagttta tccatttttc cggacgtatt atgagaagag
    18121 agaatagacc aggcaatagt gccttttttt gcttcttctt tttttatttc gccggtgaaa
    18181 tgttcttcgg caattatttc gttattgctg gcgagaaaca cgcctttttc agataacttg
    18241 atcatcaaat gctccagata tctgcgcacc gtgaagtgcg cagaaagata ataaatggat
    18301 atactctaaa taattcgagt tgcaggaagg cgacaagcga gtgaatcccc aggagcttac
    18361 ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg aagtatgacg
    18421 agtatattta cagcatgttc cagccgatca gcatattcca ccaccacacg ccgagggtga
    18481 tatgcaccag gaaggttaat atcgtcagta ccgcaccgac caaccaccag gatttaatat
    18541 cgttgtaacc cacgccaaag atgaccggac ctgccgcgcc gccatagtga gtaaccatgc
    18601 cgccatagga gttagagaac aacagtgcca gcgcggttaa cattaacggt gcgccggaga
    18661 cattcgccag catggcaaac acaggtaaca tagcaacgat ataggcacta ccagaagcga
    18721 agaaataacg cacgatgatg ctgaggaaaa taataacgaa gaaagcaacg ttaccgtgac
    18781 catcaaatgc caggttattt ttaaagactt cagctaacca ttcgaagaat ttaactttcg
    18841 ataataagga gcttaagccg ataataccgc cgtaccagat taaggtattc cagccgcctt
    18901 tatttttaac cacgtcttcc caggtaacga tacccagcag cagcatagtt gccataacaa
    18961 cgattgccac ggtggattca tcaaccccca gagacttact gaaaatccag cccagcagcg
    19021 ccagcacaaa gacaccgagc agcatttttt cgcggatttt catcggtccc agttcggcaa
    19081 ggcccgcttt agcgatggtt ttgttatcca ccttcttaat ttctggtgga tacatggtgt
    19141 aaatcaccag cggggtgacc agcagcataa tgatgcccgg caatccggcg gctaacgccc
    19201 atccacccca gctaatttgc aggtgcagaa tgtcgttgat cattttcagc gccagaatgt
    19261 tccccgccat tgcagtaaag aacatatagc tggtggtttt ggtgaccatg taaatggaca
    19321 tcatcaggta atgtccaaca cgacgcggac ttttttccgg ttctgatccc aaagccactg
    19381 ccacgctgtt gatgatcggt aacacgatgc cgcccgcacg cgcggtgtta gacggtgttg
    19441 ccggagccag taccagatcg agaaataccg taacgtaacc cagacccagg gtagtgctac
    19501 caattttacc aatcagcaga taggcaatac gtttacctaa gcctgtggtt acaaacgcgg
    19561 cgcttaaagt aaacgcagaa aacaccagcc aggtggtacc ggaagagtaa ccgcttaata
    19621 cggcggtggt tttaaattcc ccacctgata agttacccac cactaccatc gatgcggcga
    19681 cggcaattaa cagtacgaca ggttccggga aaggcttgat aaccagcccc acaatggccg
    19741 ccaggtaaat accaaaaagc acccacgcca gttcgcttaa tcctgccgga gctggcataa
    19801 agccaataat acatgggatc gctaatatca gaattagctt ccataacgat tttttattca
    19861 tttaatatcc tgttctttct gtatgactca tacagaattc ctttattgat tgtcattatc
    19921 acgacaacaa aatttttcag ggaagataaa cttcaccgga aaatattttt cttgtcgtcc
    19981 gaataacaga tgcgcgtaac gtcgtggcat cctgaccttc attacttaaa tgaacgtcga
    20041 gcgcaccact ggggtgttca atattgatat tgccgtatcc tacagaaggg acgatttgtc
    20101 gggtgacggt gccttccaat gcacaactac tggaaatagc aatagcaccg gttatcgcca
    20161 aagcgcgatg gcaagaatgc ggcataaaat aacgcacatt aattgccccg cctttctgcg
    20221 ctggagaaat aagtacaggt ttagggataa ccatattact gacatcgcct aagcccattg
    20281 ctttacccgc ttgtagacgg atagattcaa tgcgggctaa taatgctttg tcggcatcca
    20341 gctctgccgg taattcatag cctgtttttc ccagatattc agcaggaata atgacaactg
    20401 gcatcgccat atcgatacag gtcaccggga catcgtcaaa ataatcaatc tgattatccg
    20461 tcgggaagac ttttccggtt ttcgttccgg cggcattcag gaaagtgagc gcaaccggtg
    20521 cggcagtacc cggtacgccg tcaattctgg cgctaccctc gtactcgaca acaccatttg
    20581 acgtttgcac atcagcttcg ataaatgtac ccgtattgac gttgcggata cgtacgcggg
    20641 taactggcga agtcgctgca atcaaaccat tttcaatggc gaatgcccca acgccagaca
    20701 gcatattgcc gcagttaggc gtggtatcga cacgttgctc atggacgata acttgagcaa
    20761 acagataatc gacatctgca cgcggatcgc tggagcggct aataatggcg actttactgg
    20821 tcagcggatt accgccgcca ataccgtcaa tttccagatc gttaccggaa cccataattg
    20881 ccatcaatat tttatcgcgc tgcgtttgat cttcgggtag atgttccgct aacaggaacg
    20941 cgcccctcga ggttccacct cgcatcatca cgcagggtat ttttttcata ataatgccct
    21001 caatttaaaa acataactta ttgcgatatg gttacattaa gggcaaagca tctctaatga
    21061 ctttagctca acgattaata acattttttc attaatttaa gttggctaaa tgcagttaaa
    21121 atttttaact gccagtcacc caaaatcgtc gcttccattt tccggagtaa taatgaagca
    21181 tgaattatca agtatgaagg catttgtcat actggcagag tccagttcat ttaacaatgc
    21241 tgctaaatta ctcaatatta cgcaacctgc attaacgcga agaataaaaa agatggagga
    21301 agatttacac attcaactat ttgagcgtac aactcgcaag gttacgttaa caaaagcagg
    21361 aaaaaggttg ctcccggagg cgcgggaatt aataaaaaaa ttcgatgaaa cgctttttaa
    21421 tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta ttccgaccgc
    21481 agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc ccaatattaa
    21541 agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt gtaacgaatc
    21601 agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta ccccactggt
    21661 caacgagccg ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa agcagcttgt
    21721 agaatggcag gagttagtgg gttacaaaat gattggcgta cgctcctcca gcggcaaccg
    21781 actattgata gaacaacagc tagccgacaa gccctggaag ctggactggt tttacgaagt
    21841 gcgtcatctt tcgacgtcat tagggctggt ggaggcggga ctggggattt cggcgcttcc
    21901 tggtctggca atgccccatg cgccctattc ttcgattatt ggtatcccgc tggtggaacc
    21961 ggttatacgt cggacattgg ggattattcg ccgcaaagat gccgtgcttt ctccggcagc
    22021 tgaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca atttgtggac
    22081 caatatcgtc gaacgccaga gacatgcgtt acaggagata ggttaataca ccggcgcaga
    22141 agagactgcg ccgggtaaat cagcggttag tgtgcgttaa ccaccaccca cattggtccc
    22201 tgcccgaccg catagcggcc tttttcatgc agtagcccct gctcgccaac aatttcgtat
    22261 accgagatgt ggtgagattt ttgcccggcg gcaatcagat acttgccgct gtgatcaaca
    22321 ttgaagccgc gcggctgggt ttcagttggc tggaagcctt ctttactcaa cacgctgcca
    22381 tcttccgaaa cgctgaaaac agtaatcaga ctggcggtac ggtcgcaggc gtataaatga
    22441 cgaccatccg gggtgatatg aatatcagcc gcccaacggg tgtcggagaa gttttccggc
    22501 atcatatcca gcgtctggac acattcgata ttgccatgcg gatctttcag ttcccagaca
    22561 tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg atggaatacc
    22621 atatggcgtg ggccggcccc ttcaacggta gtcacttccg cagggtcctg cgctaccaga
    22681 tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc cggaacccac
    22741 aacgtacggt tgtccggtga gatattggcg gaatggcaac cgtccagccc ctcgaccaca
    22801 tcgacgacgc ccactggcag gccatcttcc agacgcgtta cgctcacgtt acccgcattg
    22861 taagaaccga caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt cgggctaccc
    22921 ggcagcgcag actctgcagc aaaggtcagc gcgccatcat ccggtgcaat acgatacgcc
    22981 agaacacgaa actccggacg gacaccaacg tagaggtaac gtttgtccgg gctgaccacc
    23041 atcggctgca cctgccccgg cacatcgact acctgtgtca gcgtcagtgc gccttcatga
    23101 ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac tgtttgcttc
    23161 atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc gcttttggcg
    23221 gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt accattcgcg
    23281 cagacgaaaa ttcttaacct taatctggaa tacgccatga ccacacgcgt gattgctctc
    23341 gacttagacg gcaccttatt gaccccgaaa aagaccctgc ttccttcatc gatagaagcc
    23401 ctggcccgtg ctcgcgaagc aggctaccga ttaatcatcg tcacaggtcg ccatcacgtc
    23461 gctattcatc ctttttatca ggcgctggcg ctggatacac ctgctatttg ctgtaacggc
    23521 acctatttgt atgattatca tgcaaaaacc gtactagaag cggacccaat gcctgttaac
    23581 aaagccctgc aactcattga gatgctgaat gaacaccaca ttcacggtct gatgtatgtg
    23641 gatgatgcga tggtttatga gcacccgacc ggacatgtca ttcgcacgtc taactgggcg
    23701 caaaccctgc cgccggaaca gcgtccgact ttcacacaag tcgcttctct ggctgaaacg
    23761 gcgcaacaag ttaacgccgt atggaagttc gccctgacgc acgatgacct gccgcaattg
    23821 cagaattttg gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg gtcctggcac
    23881 gatcaggttg atattgcacg cggcggcaac agcaaaggta aacgtttgac gaaatgggtt
    23941 gaggcgcaag gctggtcgat ggaaaacgtc gtggccttcg gcgataactt caatgatatt
    24001 agtatgctgg aagccgctgg tacaggcgtg gcgatgggca acgccgatga cgcagtaaaa
    24061 gcgcgcgcca acattgtgat tggtgataac accaccgaca gcattgccca gttcatttat
    24121 agccacctga tttaatcagg cggttatcga cacactttta atttgcgcat acagccacag
    24181 gccaggtttg atcgccagtt catccctggc ccacgggctg attcgcgccc acagcgtttt
    24241 accgccgact tccagctcca cttccacctg gccgttgtcg tcataactat taacaacttt
    24301 tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca gcgagacatc
    24361 ggaagcctga atgcggatgc gtagcgcagc ttgcagcggt tcgtccagct tattgaccca
    24421 caaatgctga tcgcccagcg ccagcgcggt catcgcgtaa tgcggatgat gctcaagtac
    24481 tgtcactttc agaatgctac tttgttgctc tttcggtagc cacggattca tcacgctact
    24541 accccagact tcttccagcg cgccaaaggc tttcacctga ccgttttcca gtaccatcac
    24601 tctgtctgcc agatggagga tttcatccag cgaatggctg acatacaaca tcggaatgtt
    24661 gatttcccgc gtcagacgtt gcagataagg caacagttcg cgtttacgcg gaatatccag
    24721 tgacgccagc ggttcatcca gcaacagcaa ttccggtgct gtcagcaaag cccgaccaat
    24781 cgccacgcgc tgtttttcgc ctccggacag gctgcctggt aaacgatcaa gcaacggttc
    24841 aatgcctaaa agcgccacca gcttatcgaa ctgattgacc atgcttttcg ccatgccgta
    24901 gcgcaaattg ccacgcactt tataatgcgg aaacagccgc gcatcctgaa acacatagcc
    24961 tacgcgacgc ttttctggcg tcaggcagat acctttatcg gcgtcattta gtacccgacc
    25021 attgaggaca atccgccctt tttgcgggcg cgtcagccca ctgatggcgt taatcagcga
    25081 ggtttttccg gcaccagaga caccaaaaat agcggtgatg ccattggcgg gcagcgtttc
    25141 attaatcgtc aggcaatggt tgcccaacgt ctgggaaaaa ttcagttcca gcatgattag
    25201 cgccccgccc gctcacggct gattctggcc agccattctg aaatcaacag ggagatcatc
    25261 gccagcgcaa tagaaataat acacagtctc gccgcgccac tttccccgcc aggggtctgg
    25321 atcagggtat acatggcaga aggaatggtc cgcgtttcac caggaatgtt cgacacaaag
    25381 gtgatggttg cgccaaattc accgagcgaa cgagcgaatg ccagtaccgt accaacaata
    25441 attcccggca acgtcagcgg taaggtaata gtgaagaaga cacgccagcg cccggcgccc
    25501 aacgttcttg cggcctgctc cagtttgata tcaacacctt ccagcgccag acgaatcgcc
    25561 cgcaccatca gcggaaaaga catgacagcg gcagcgagaa ccgcaccgcg ccagctaaac
    25621 gcgaaagaaa gaccaaacca gtcatacaga cgttcaccga taaatccgcg ccgtcccatc
    25681 gaaactaata ataggtaacc gacgaccacg ggcggtaaca ccagcggtag atgcagtaca
    25741 ctgtcgagca gagctttgcc cggaaacgtg caacgtacca gtaaccaggc aaaaaagatc
    25801 ccaaacggca ggctaaacag cacagccagg gaagaaactt tcaggcttaa taaaactgcc
    25861 tgccattctg gatcggtcag tatcattact tggttgtaaa tccgtaacgt ttaaagattt
    25921 ccgcagcctg cggccccttc agataatcgt aaaatgcttt cactgtcgca ttgttatgcc
    25981 cttccacaac agcaaccgga tattccactt ttttatgtga atcttccggg aaggtggcaa
    26041 ccacttttac ccctttgctg gcaactgcgt cagaaccgta aacaataccc agcggcgctt
    26101 cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc agtttcgggg
    26161 agagcgtatc ccatgcgccc agtttttgca gtgcttcttt cgcataaatg ccagcaggga
    26221 catgttccgg atcaccaacc gcgaggcgac cgccattcag cagcgaagtc cagttggttt
    26281 tgctgtcgat ggtgaaatct ttctgctcgc tggctttcgg tgctacaacg accaggctat
    26341 tgccgagcag tgtctggcgc gaagccgtat cgatcgcttt tttatcaacc gcataatcca
    26401 tccatttctg atcggcagaa ataaacagat ccgcaggcgc acccgcttca atctgacggg
    26461 ccaaagtaga tgacgaagcg aaagaagaaa ctacatccac gcctttctct tttttatact
    26521 gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc gtgattttcc
    26581 cttcatctgc cagtgcattg ccagcaacag cgaaagagag tgccgccccg gtaaacaagt
    26641 tcaaccattt acgagccatt ggtaacccct taatgtaacg ttatgtaggc gtcaatataa
    26701 cggcaagttc ggaattttcc cagcagttat tgctaacctt tgtgaggtag atgagaaaaa
    26761 atatcggcag aaaaagcagg aagttgagaa aaagaaaatg cccgactaag cgggcattca
    26821 gggaatcaat gattttgtcc gggctggtct tttttaccaa caccagaaaa gatgttgaaa
    26881 acttcaccaa gaccgtaaat cagacccagg atgatggcca tcacgacagg taccatgatt
    26941 acggcgaata ccagactttt taataactct aacatggtca actccagata tagtcatgag
    27001 actattctaa ccgctaagca cagaaaagca ctcccctttt gtgcggtcag ctttgcgtgg
    27061 cgttcgtttt ccgtcacaat aagacttttg ccaggatatt gttatgcagg ccgaaatcct
    27121 tctcaccctt aagctccaac aaaaattatt cgccgacccg cgccgcattt cgctactaaa
    27181 acacattgcg ctttccggtt ccattagcca gggagcgaaa gatgccggta tcagctataa
    27241 aagcgcctgg gatgccatta acgagatgaa tcagttaagt gagcatattc tggttgagcg
    27301 cgcaacaggc ggtaaaggtg gaggcggcgc agtactgacc cgctatggtc agcgactgat
    27361 tcagctctat gacttactgg cgcaaatcca gcaaaaagcc tttgatgtgt taagtgacga
    27421 tgacgccctg ctgctgaaca gcctgctggc cgcgatctca cgtttttcac tgcaaaccag
    27481 cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat catgatgacg ttcaacagca
    27541 tgttgatgtc ttactggctg atggaaaaac acgcctgaaa gtcgcaatta ccgcacaaag
    27601 cggcgcacgt ctggggctgg atgaaggcaa agaagtgttg atattgctaa aagcgccgtg
    27661 ggtagggatt actcaggacg aggcggtcgc gcaaaacgct gacaaccaat taccgggtat
    27721 tattagtcat attgagcgcg gcgcagagca gtgcgaagta ttaatggcgc tacccgacgg
    27781 gcaaacactg tgcgccacag tgccgataaa tgaagcgact tctcttcagc aaggacagaa
    27841 tgtcacggcc tactttaatg ccgacagcgt gattatcgcc acgctgtgct aagcgtgttg
    27901 acaatttgtt atgaaacacg tatccctgtc agtaatcgct gcacaaaatg ggatataaaa
    27961 tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttgg cgacacaaaa acgctgcaat
    28021 tgcctcggct aacgttaaac gcgggtgata gttgggcgtt tgtcggttcg aatggaagcg
    28081 ggaaatcggc gctggcccgc gcgctgtcgg gggaacttcc gcttttgaaa ggtgaacggc
    28141 aaagccagtt ttcccacatc actcgtctct ccttcgagca attgcaaaag ctcgtcagcg
    28201 acgaatggca gcggaataac accgatatgc ttagtcctgg cgaagatgac accgggcgta
    28261 ccacggctga gatcattcag gatgaagtaa aggatactcc gcgttgcatg caactggcgc
    28321 agcagttcgg tattaccgcc ctcctcgacc gacgctttaa atacctttcc actggcgaga
    28381 cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc tgacttgttg atccttgatg
    28441 aaccgttcga tggcctggat gttgcctcac atcagcaact ggctgagcta ctcgcctcgt
    28501 tacatcagtc cggtattact ctggtactgg tgctcaatcg cttcgatgag atcccggaat
    28561 ttgtccagtt tgctggcgtg ctggcggatt gcacgttagc ggaaactggc gctaaagaag
    28621 aactgctcca acaagcactc gttgcgcaac tggcgcatag cgaacagctt gaaggtgtgc
    28681 aactgccgga gccggatgaa ccttcagcac gtcacgcctt acccgccaac gaaccgcgca
    28741 ttgtgctgaa caatggcgtg gtttcttata acgaacgccc cattcttaat aaccttagct
    28801 ggcaggtgaa tccaggcgaa cactggcaaa ttgtcgggcc aaatggcgcg ggaaaatcga
    28861 cgttattaag cctgattact ggcgatcatc cgcaaggtta cagcaacgat ttgacgcttt
    28921 tcggacgacg tcgcggcagc ggcgaaacca tctgggatat caaaaagcat atcggttacg
    28981 tcagcagtag tttgcatctg gattaccggg tcagcactac tgtgcgtaat gtgatccttt
    29041 ctggctattt tgattcgatt ggcatttatc aggccgtttc ggatcgccag caaaaactgg
    29101 tgcagcagtg gctggatgtt ctcggcattg ataaacgcac ggctgacgct ccgttccata
    29161 gtctttcctg gggacagcag cgtctggcgc tgatcgtccg cgcgctggtg aaacatccga
    29221 cgttgcttat tctcgatgaa ccactacagg ggcttgatcc gctcaatcgc cagcttatcc
    29281 gccggtttgt tgatgtgctg attagcgaag gtgaaatgca attgttgttt gtttcgcacc
    29341 acgctgaaga tgcgcctgcc tgtattaccc atcgtcttga gttcgtgccg gacggtgact
    29401 tttatcgcta tgcgctgaca aaaataaact gagtcggtag tgctgaccgt gccggaggcg
    29461 gcgtaaacgt gttatccggc caatggtttg acgcatgcag gcatgaaacc gcgtcttttt
    29521 ttcagataaa aagcgcaatc attcataaac cctctgtttt ataatcactt aatcgcacat
    29581 taaaaacggc taaattcttg tgtaaacgat tccactaatt tattccatgt cacacttttc
    29641 gcatctttgt tatgctatgg ttatttcata ccataagcct aatggagcga attatgagag
    29701 ttctggttac cggtggtagc ggttacattg gaagtcatac ctgtgtgcaa ttactgcaaa
    29761 acggtcatga tgtcatcatt cttgataacc tctgtaacag taagcgcagc gtactgcctg
    29821 ttatcgagcg tttaggcggc aaacatccaa cgtttgttga aggcgatatc cgtaacgaag
    29881 cgttgatgac cgagatcctg cacgatcacg ctatcgacac cgtgatccac ttcgccgggc
    29941 tgaaagccgt tggcgaatcg gtacaaaaac cgctggaata ttacgacaac aatgtcaacg
    30001 gcaccctgcg cctgattagc gccatgcgcg ccgctaacgt caaaaacttt atctttagct
    30061 cctccgccac cgtttatggc gatcagccca aaattccata tgttgaaagc ttcccgaccg
    30121 gcacaccgca aagcccttac ggcaaaagca agctgatggt ggaacagatc ctcaccgatc
    30181 tgcaaaaagc ccagccggac tggagcattg ccctgctgcg ctacttcaac ccagttggcg
    30241 cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat tccgaataac ctgatgccat
    30301 acatcgccca ggttgctgta ggccgtcgcg actcgctggc gatttttggt aacgattatc
    30361 cgaccgaaga cggtactggc gtgcgcgatt acatccacgt aatggatctg gcggacggtc
    30421 acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt acacatctac aacctcggcg
    30481 ctggcgtggg cagcagcgtg ctggacgtgg ttaatgcctt cagcaaagcc tgcggcaaac
    30541 cggttaatta tcattttgca ccgcgtcgcg agggcgacct tccggcctac tgggcggacg
    30601 ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg cacactcgat gaaatggcgc
    30661 aggacacctg gcactggcag tcacgccatc cacagggata tcccgattaa ggaacgacca
    30721 tgacgcaatt taatcccgtt gatcatccac atcgccgcta caacccgctc accgggcaat
    30781 ggattctggt ttcaccgcac cgcgctaagc gcccctggca gggggcgcag gaaacgccag
    30841 ccaaacaggt gttacctgcg cacgatccag attgcttcct ctgcgcaggc aatgtgcggg
    30901 tgacaggcga taaaaacccc gattacaccg gcacttacgt tttcactaat gactttgcgg
    30961 ccttgatgtc tgacacgcca gatgcgccag aaagcaacga tccgctaatg cgttgccaga
    31021 gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga tcacagtaaa acgttgccag
    31081 aactgagcgt tgcggcattg acggaaatcg tcaaaacctg gcaggagcaa accgcagagc
    31141 tgggaaaaac gtacccgtgg gtacaggtct ttgaaaacaa aggcgcggcg atgggctgct
    31201 ctaacccgca tccgcacgga cagatttggg caaatagctt cctgcctaac gaagctgagc
    31261 gcgaagaccg tctgcaaaaa gaatatttcg ccgaacagaa atcgccaatg ctggtggatt
    31321 atgttcagcg cgagctggca gacggtagcc gtaccgttgt cgaaaccgaa cactggttag
    31381 ccgttgtacc ttactgggct gcctggccgt tcgaaacgct actgctgccc aaagcccacg
    31441 ttttgcggat caccgatttg accgacgccc agcgcagcga tttggctctg gcgttgaaaa
    31501 agctgaccag tcgttatgac aacctcttcc agtgctcctt cccctactct atgggctggc
    31561 acggcgcgcc atttaatggc gaagagaatc aacactggca gctgcacgcg cacttttatc
    31621 cgcctctgtt gcgctctgcc accgtacgta aatttatggt tggttatgaa atgctggcag
    31681 aaacccagcg agacctgacc gcagaacagg ccgcagagcg tttgcgcgca gtcagcgata
    31741 tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa agaaaaaaca caatctctgt
    31801 ttgccaacgc atttggctac cctgccactc acaccattca ggcgcctggc cgcgtgaatt
    31861 tgattggtga acacaccgac tacaacgacg gtttcgttct gccctgcgcg attgattatc
    31921 aaaccgtgat cagctgtgcg ccacgcgatg accgtaaagt tcgcgtaatg gcagccgatt
    31981 atgaaaatca gctcgacgag ttttccctcg atgcgcccat tgtcgcgcat gaaaactatc
    32041 aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca actgcgtaac aacagcttcg
    32101 gcggtgtgga catggtgatc agcggcaatg tgccgcaggg ggccgggtta agttcttccg
    32161 cttcactgga agtcgcggtc ggaaccgtat tgcagcagct ttatcatctg ccgctggacg
    32221 gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca gtttgttggc tgtaactgcg
    32281 ggatcatgga tcagctaatt tccgcactcg gcaagaaaga tcatgccttg ctgattgact
    32341 gccgctcact ggggaccaaa gcagtttcca tgcccaaagg tgtggctgtc gtcattatca
    32401 acagtaactt caaacgtacc ctggttggca gcgaatacaa cacccgtcgt gaacagtgcg
    32461 aaaccggtgc gcgtttcttc cagcagccag cgctgcgcga tgtcaccatt gaagagttca
    32521 acgctgttgc gcatgaactg gacccgatcg tggcgaaacg cgtgcgtcat atcctgactg
    32581 aaaacgcccg caccgttgaa gctgccagcg cgctggagca aggcgacctg aaacgtatgg
    32641 gcgagttgat ggcggagtct catgcctcta tgcgcgatga tttcgaaatc accgtgccgc
    32701 aaattgacac tctggtagaa atcgtcaaag ctgtgattgg cgacaaaggt ggcgtacgca
    32761 tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat cccggaagag ctggtgcctg
    32821 cagtacagca agctgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt
    32881 acgtttgtaa accatcacaa ggagcaggac agtgctgaac gaaactcccg cactggcacc
    32941 cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac gcagggatgg tagtcacgct
    33001 gatggactgg ggtgcgactt tactttccgc ccgtattccg ctttccgatg gcagcgtccg
    33061 cgaggcgctg cttggctgcg ccagcccgga atgctatcag gatcaggccg catttctggg
    33121 ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc cgttatacct ttgacggtga
    33181 aaccgtgacg ctttcaccaa gtcagggcgt caaccaattg catggcggcc cggaagggtt
    33241 cgacaaacgt cgctggcaga ttgtgaacca gaatgatcgt caggtgctgt ttgccctgag
    33301 ttcagatgat ggcgatcagg gtttcccggg taatctcggc gcgacggtgc aatatcgtct
    33361 gaccgacgat aaccgtatct ccattactta tcgcgccaca gttgataaac cttgcccggt
    33421 gaatatgact aatcacgtct atttcaatct tgacggcgag cagtctgacg tgcgcaatca
    33481 taagttgcag attctggcgg aagaatatct gccggttgat gaaggcggca ttccgcacga
    33541 cggcctgaaa tctgtcgccg gaacctcttt tgatttccgc aacgccaaaa tcatcgccag
    33601 tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt tacgatcacg cgttcttgtt
    33661 acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc tggtcagcag atgaaaaatt
    33721 gcagctgaag gtctacacca ccgctccggc tctgcaattc tactccggca acttcctcgg
    33781 cggcacaccg tcgcggggaa ccgaacctta cgccgactgg caagggctgg ctctggaaag
    33841 cgaatttctg ccggacagcc cgaaccaccc tgaatggccg caaccggact gcttcctgcg
    33901 tcctggcgaa gagtattcca gcctgacgga atatcagttt attgctcagt aatgttaagc
    33961 cctcgtcatg agggctttat cttcatattg ttcaaatcac cagcaaacac cgacatattt
    34021 gcaactcaat attcacaaca accttacact gcgacactat tttcgctatg gttatgcgta
    34081 agcattgctg ttgcttcgtc acggcaatat aatgagaatt attatcatta aaagatgatt
    34141 tgaggagtaa gtatatggct gtaactaagc tggttctggt tcgtcatggc gaaagtcagt
    34201 ggaacaaaga aaaccgtttc accggttggt acgacgtgga tctgtctgag aaaggcgtaa
    34261 gcgaagcaaa agcagcaggt aagctgctga aagaggaagg ttacagcttt gactttgctt
    34321 acacctctgt gctgaaacgc gctattcata ccctatggaa tgtgctggac gaactggatc
    34381 aggcatggct gcccgttgag aaatcctgga aactgaacga acgtcactac ggtgcattgc
    34441 agggtctgaa caaagcggaa accgctgaaa agtatggcga cgagcaggtg aaacagtggc
    34501 gtcgtggttt tgcggtgact ccgccggaac tgactaaaga tgatgagcgt tatccgggtc
    34561 acgatccgcg ttacgcgaaa ctgagcgaga aagaactgcc gctgacggaa agcctggcgc
    34621 tgaccattga ccgcgtgatc ccttactgga atgaaaccat tctgccgcgt atgaagagcg
    34681 gtgagcgcgt catcatcgcc gcacacggta actcgttacg tgcgctggtg aaatatctcg
    34741 ataacatgag cgaagaagag attcttgagc tgaatatccc gaccggcgtg ccgctggttt
    34801 atgagtttga cgagaacttc aaaccgctga aacgctatta tctgggtaat gctgacgaga
    34861 tcgcggcgaa agcagcggcg gttgctaacc agggtaaagc gaagtaaacg tcattcgttt
    34921 aaaaatgaga aagccgaccg caaaatgagt cggctttttt atttttcgtt gctaccaatg
    34981 gagcacatcg cctgatgcga cgctgcgcgt tttatcaggc ctccggtgat taatcggata
    35041 cgccactctg acggcgcatc cgacaattaa actttacccg cgacgcgctt ttactgcatt
    35101 cgccagttga cgtaacagag catcggtatc atcccagcca atgcaggcgt cggtgatgct
    35161 cttaccgtag gccagcggtt ccccgctctc gaggctctga ttgccttcca ccagatggct
    35221 ttccaccatc acgccaataa tggccttttc gccaccggca atctgctggc aaacgtcagt
    35281 acaaacatcc atctgctttt tgaattgttt tgacgagtta gcatggctga aatcgatcat
    35341 cacctgtgct ggcaggcctg ctttgttcag cccttctttc acttcagcaa cgtgcttcgc
    35401 gctgtagtta ggctctttac caccgcgcag aatgatatgg caatcgccgt taccgctggt
    35461 attcacaatc gccgaatgac cccatttggt cactgaaagg aagcagtgcg gcgcaccggc
    35521 ggcattaatg gcatcgatag ccactttaat cgtaccatca gtgccatttt tgaaacctac
    35581 cggacaagaa agaccagacg ccagttcgcg gtgcacctgc gattcggtgg tacgtgcacc
    35641 aattgcgccc cagctcatca ggtcagcgag atattgtggg gtgatcatat ccaggaattc
    35701 acccgccgct ggcagaccgc tgtcgttaat atcaagcagc aatttacggg caatacgcag
    35761 accgtcgttg atctggaagc tgttatccat atgcggatcg ttaatcagcc ctttccagcc
    35821 caccgtggta cgcggctttt caaaatagac gcgcattacg atttccagct catctttcag
    35881 ctcttcacgc agcgccagca agcgagcggc atactcttta gccgcgacag gatcatgaat
    35941 tgagcatggg ccaatcacca ccaacaggcg atcatcatta cctttcagga ttttatggat
    36001 cgcttttcgg gcatgggcga ccgtattcgc ggcattttca gtagcgggga atttttccag
    36061 caatgcgaca ggaggaagta actctttgat ttctttgatg cgtaaatcgt cgttctgata
    36121 attcatgtct gttccagtgt tgccatactt atcttagtga atgcaatact tccaatctat
    36181 atcttccgtc agaatgtgta aacggggttt tacactatga acggattaat cctggaatta
    36241 gcaagaaaaa cgccagattg tcgcgaaaaa cgagatctct cctacaattt ctaactgtaa
    36301 ctcctttgtt tgttaattat ttcaagattc tctgctgggt atctttgcct ggctgaaatc
    36361 ttaaaccatt gccttatatt cacctgcaaa tgcactgttg gaagaggtta tccgacataa
    36421 cgaccataac aggagcatcc tatgaaaatg acaaaactgg ccacactatt tttgactgcc
    36481 actctaagcc ttgccagcgg tgccgcactg gctgccgata gcggagcgca aagcaataac
    36541 ggccaggcaa acgccgcagc tgatgcgggc caggtagccc ctgacgcccg tgaaaatgtc
    36601 gcgccaaata acatagacaa taacggggta aataccggtt ccgacggcac aatgctgcat
    36661 ccggatggtt cttcaatgaa caatgacggc atgaccaaag atgaagagca caagaacacc
    36721 atgtgtaaag atggtcgctg cccggatatc aataaaaagg tacaaactgg tgatggcatc
    36781 aataatgatg tcgataccaa aaccgacggt accacgcagt aaccctgcaa aatagccaat
    36841 aatgggagag cggagtctct cccttttaat ttttcccttc tacaagcagt atgatgtcat
    36901 aaagttaagt gagaataatt aagggatgac tcgtcatggc gcactcacac tcacacgctt
    36961 cctcacacct gccagaagat aataatgctc gtcgcttgtt gtatgctttc ggcgtgactg
    37021 ccgggtttat gctggtagaa gtcattggcg gttttctttc cggttctctg gcattgctgg
    37081 ccgatgcggg tcatatgttg accgatactg ccgccctgct tttcgcccta ctcgccgtgc
    37141 aattttcacg tcgacctccc accattcgcc acactttcgg ttggctaaga ttgaccacgc
    37201 tcgcagcatt tgtgaatgct atcgccctgg tggtgattac cattttgatt gtctgggagg
    37261 cgatagaacg cttccgcaca ccgcgtccgg tcgagggcgg catgatgatg gcaattgccg
    37321 tggctgggct gttggcaaat atactttctt tctggttact tcatcacggt agcgaagaga
    37381 aaaacctcaa cgtgcgagca gcggcactgc atgtgctcgg ggacctgctg ggttcggttg
    37441 gggctattat tgccgcgttg attattatct ggaccggctg gacacctgct gaccccattc
    37501 tctcaatact ggtgtcgctt ctggttctgc gtagcgcgtg gcgactgttg aaagatagcg
    37561 tgaatgaatt acttgaaggt gcaccggtat cgctggatat cgccgaactg aagcgtcgta
    37621 tgtgccggga aatcccggaa gtccgcaatg tgcaccatgt acatgtatgg atggtaggcg
    37681 agaagccggt gatgacgctg catgtgcagg tgatcccgcc acacgatcac gatgccttgt
    37741 tagatcagat ccaacactac ctgatggatc actatcagat tgagcacgcc actattcaga
    37801 tggaatatca accttgtcat gggccagact gccacctgaa tgaaggggtg tctggccatt
    37861 cacatcatca ccattaatgg gacagcgcgc gtgaacctct ttcgcgtgcg ctgttgatcc
    37921 acatccggct gccgttgagg gcaataaagg tcagaatgat gtactccaga gacatggcgt
    37981 aaacgccctg aagtgcaaaa ataacgacgc taatcacgtt aataatcacc cacaacagcc
    38041 agttttccac atacttacgc gtcatcagaa tcattgccac gatagataac accatcatgc
    38101 atgaatccca gaacgggaaa gcgtccggct gtagttcagg catcgcaacc tgtaatccta
    38161 atgcttgcat gatcatgact gccacgcggg tcaaaaatgc aaacaccgga ttgataaata
    38221 ccgtcatcag accaatcgaa acaacgcaaa ccgccaacca gcttagcgcc ttcggcaatg
    38281 gcaaccagcg aattttcaac tccgcctcgt tctgactggt ttgtcgcgac cacgcatacc
    38341 agccgtaaat attcgcggca aagaaaaaca cctgtaataa caggctggca tacagctgaa
    38401 tctgaaagaa aataatgcca aacaaggtga cgttgatcag gccaaagaag tagttactga
    38461 tcttctccag actggcaagg ccaatacaca gcaacccggc gatcgtgcct accgcttcga
    38521 tccatgagag atcataaccg cctgccccta ttggtatatg taccaggata ttctgcacac
    38581 taaaaaaatc catcgttccc ccaaagcgta atgtgacgcc ttattatcaa ttatccacgt
    38641 agtgtagccg caaaatccag catacgattg agcggcacca gcgccctctc tcgcagcctt
    38701 tcatcaacat aaacctcgtg attgcttcct tccagttcta atgcctctgc gatggcctga
    38761 aggccattca tggccatcca cgggcaatgc gcgcagctgc gacaggttgc gccctcaccc
    38821 gcggttggtg cttccagtaa ctctttatct ggcaccgcct gctgcatttt gtagaaaatg
    38881 ccccgatcgg ttgccacgat aagccgctga tgtggcaatg ttttcgcagc agcgatcagt
    38941 tgactggtgg aaccgaccgc atccgccatc tctacaatag cttgcggtga ttctggatgc
    39001 accagtatgg cagcatccgg gtattcttct tgcaagcggg ttaacgcttg agtcttaaat
    39061 tcatcatgca caatgcaggc accctgccag cataaaatgt ctgcgcccgt ctgtttttgc
    39121 acgtaacgcc ccagatgttt gtcaggtgcc cagatgattt tttcacccaa actatccaga
    39181 tgatcaataa gttcgacggc aatgcttgaa gttaccaccc aatctgcgcg cgcttttacc
    39241 gcagcagaag tgttggcgta gacgacgaca gtacgatcgg gatgggcatc gcaaaatgcg
    39301 ttaaattctt caacagggca gccgagatcc agtgaacatt cagcctgaag tgtcggcatc
    39361 agaattgttt tttccggact gagaattttg gcggtttctc ccataaatct caccccagca
    39421 actaacaacg tagaagcggg atgctttgca ccgaagcgcg ccatttccag agaatcagaa
    39481 atacagccac cggtttcttc tgccagttgt tgaatttcgg gatcggtata gtagtgggca
    39541 accatcaccg cattacgttc ttttagtaga cgttttatct tctcgcggta atacgctttt
    39601 tcatcaatgc ttaacggcgt cggcttcggg gggaaaggat aaatcgccgt gtctggatca
    39661 aacatcacgc tcatcttact atctcgtttt acaggcttaa cgttaaaacc gatttaagcg
    39721 tgatattcgc tattatcggc gtaatttgtt ttatatgcta aacaagatag tcgaatggtg
    39781 gagaaaagtc actcgaatta cgtatgagat gccgaatgcc acactttgcg tcttattcct
    39841 ccgacgtaga gttgtttacg cagcaaacgc ggagtatgtc atcagggtca agaattgaaa
    39901 ttgagagatg cctgaatagc aaaaaagcgc ctttagggcg cttttttgca ttggtgggtc
    39961 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    40021 acgacccctt gcgggattat gctgctttaa tcattcagga tggtgggtcg tgcaggattc
    40081 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccatac
    40141 gggtgttacc tgaaagattt ctactcggaa ccaatgttta aaattggtgg gtcgtgcagg
    40201 attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc
    40261 gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
    40321 ccagctgagc taatcacccg atactacgct ggatactaca aactacttcc accgaaccca
    40381 ccgtaatcag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    40441 tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga accgccgacc
    40501 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgatact acgctggata
    40561 ctacaactaa ctatgttagt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    40621 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    40681 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    40741 ttcgatgtag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    40801 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgcgcctgt ttagcgccat
    40861 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    40921 ctttgtcctg catgatgacg ccgactttaa acatcgcatc tgcagccttc ggtgacttcg
    40981 gatagttttt cactaccgaa gcaaaataat acgccgcatc atctttttta cccttgttgt
    41041 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    41101 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    41161 caatagctgc attgtaatcc gtgtttgcat caccgctttt caccggcgcg ccagcattcg
    41221 cagcaccagc atcagctgtc ggcgtcgttg atgccgccac accgctttga tcgccgctgg
    41281 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    41341 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    41401 caatatcgga ttgattatca gagagttgtt gctggagttg ggttaaaagc tggctgtgag
    41461 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ccgacactac
    41521 tgattggtgc ctgagcaaaa gcggcccaag gggccgctat accaaccagt aacgacagac
    41581 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    41641 gacggttttt ggcatacgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    41701 aagaaacgat ggagatctgg tcagcagaaa cgcctttacc ctgcagatac atcttaacag
    41761 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    41821 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgcg tgtgcatcca
    41881 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    41941 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    42001 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    42061 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    42121 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    42181 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    42241 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    42301 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    42361 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accgttggag
    42421 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    42481 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    42541 ccggcctggt cagaagtaaa tgccaggttc tggctgtccg ggaaccaggt cggttcggta
    42601 ttgttactgc gaccatcagt tacctggcgg atctgaccag aagccaaatc cattacgtac
    42661 aggttcaggc taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    42721 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    42781 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    42841 tctggtgacc acgccggaga catcagcggt tgcggtgagc ggtgaacgac aaactggttg
    42901 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt agtctgaaca
    42961 acgtaggcaa tacgggtacg gaacgcacct ttaataccgg tcagtttttc aaatacttcg
    43021 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    43081 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcgac attgtaagaa
    43141 ccatccggat tcggagtgac ctgaccgaca accacagcgt caatgcccag cgcggaccat
    43201 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc acgatctaac
    43261 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    43321 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    43381 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    43441 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    43501 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    43561 ccggtgcctg atgttgaccg tcggaacagt caacatcgcg attacggttt gaagtccaat
    43621 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    43681 ttagctgctg ccaacgcagc ttgacaaagt gcgggatcgc caccttcagg tttgatatcc
    43741 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    43801 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    43861 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    43921 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    43981 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    44041 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgccttt
    44101 tcagcagccg ctttttctgc tgcttttttg tcggctgccg ctttctctgc tgccgctttc
    44161 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    44221 gcagcttcgg ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttcagct
    44281 ttcttcttca gcgccgcagc agcagcctcg gcttttttct gcgcttcgac tgcggctttg
    44341 gcggcttctg cttctgcttt tttcttcgcg tctgccgccg ctttcttcgc tgcttcttcc
    44401 gcagctttag catccgcttc ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc
    44461 tcttccgctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
    44521 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
    44581 tgtttctcac gcagttcttc agcagcctgc tgctccttca tcttgcgctg ctcatcagaa
    44641 cgcttcgcgc ttgattcctg gctttgcata cgtttgtact gctcaactac cgcacctgaa
    44701 tcaaccatga cagcgtcgat ggacgaacca ccgccacctc cggctgaagc ttctatattc
    44761 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
    44821 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
    44881 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
    44941 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
    45001 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
    45061 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccagacgatc
    45121 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
    45181 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
    45241 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta catccagcaa
    45301 cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
    45361 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
    45421 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt ttaggcggtt
    45481 ataagccatt accgccggaa ttgcggcgaa caaaccaatt gccgtcgcaa tcaacgcttc
    45541 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
    45601 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
    45661 cgtaccgagg aaaggaatgt gcgtttccag attttcaagt tcacgattca tggaaatacg
    45721 catcgcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
    45781 gcgcacaaac tctttgaagc cgctgtaaaa gatttgttcc gaaccgctca gattatcccg
    45841 tttcccctgg ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
    45901 aaacgcttcg gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga taataatggc
    45961 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
    46021 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
    46081 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
    46141 tcattcaaca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca
    46201 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa
    46261 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgttga
    46321 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
    46381 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacacgaac cggccatcga
    46441 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
    46501 tgctactata cgcgcgcgat tgaggtttgg gaatgagagc aagttaaggg agcgtaaaaa
    46561 tttatgggcc tgactcggcc cataaatttt taaagagaat tctgatttaa aagaagaaga
    46621 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaga
    46681 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacataa
    46741 gcagtaaacc atgccagatt tccagttcac tggtcttcgc ggcgaaacgt gatgggtccc
    46801 aaaacataca tcctgctaac agaagtgcca tcacgaagga aagcgcccgc agggggcgct
    46861 tgtacattac cgcatacaag gttgcgataa tcttactcat cagatgtctt cttgaccggc
    46921 tttgcctgat tcgacgtgct caagcgccag cgcggtgatt accccaaacg aacaggcaag
    46981 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
    47041 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
    47101 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
    47161 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
    47221 ggcatcacaa acgggaacat tgcgataccg gcagtcagga tgatgcaggc cagcgtcagg
    47281 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
    47341 ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
    47401 agccatgcgc cagcttcacg aaccacttct ttattcagcg ggttagaggc tgcgtaatgg
    47461 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
    47521 gcgaagcaga ccagcgtcac cagtgccgcc acctgagccg ttgcacgggt acgcaggtgc
    47581 agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
    47641 ctcaccacac ctgccagcag gccgaacggg ttaagcagct ggaagaagtt accggtgtag
    47701 tacagacgca gatattcatc aacgttgaat ggtacgccct gcaacaggtt accgaacgct
    47761 acaccaatca ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
    47821 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
    47881 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
    47941 ggccaggcag caaacagtgc gccgcctgca gtgatcagcc aaacctggtt accgtcccag
    48001 tgtggtgcga tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
    48061 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
    48121 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
    48181 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
    48241 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
    48301 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
    48361 gagttcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
    48421 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
    48481 cgcagcagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgca
    48541 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcgaa gtacagcggc
    48601 gctacacgtg gaatggagtc tttggttgcc tgttgaatct gagcttcagt cgcatcagcc
    48661 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga gatctttctt catgctattg
    48721 aactggtcac gaacggcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
    48781 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagttcttt cagaccgata
    48841 actggagtat ccacggaacg cgttgcaatg atgcccagcg cgtaagggat ctggatcgca
    48901 aatttgttcg tttcctcttc ctgatcagga atgccgaaca gagtaaaggc agcaggtgca
    48961 ggttgcgttt cccactcggc ttcgatagca gccagtttgg ttttctgcac gtcgcccatt
    49021 tcgtaaccgg attcatcacc cagaacaata acagacagaa cggcagccat accgaagctg
    49081 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
    49141 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagaagctac ggtgtgaacg
    49201 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
    49261 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt tgcaaccaga
    49321 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
    49381 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
    49441 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
    49501 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
    49561 gcaaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
    49621 ccggaaagga cgtagaccgt ttccataatg gccagcagga acgccatacc gagcgtcagt
    49681 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
    49741 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
    49801 aaagggtcac acgcatgccc caatacaaaa ttatttgctc gcctctatcg ctgccatttc
    49861 atcaaaagag gaaaagacaa tgataaaaaa gttacaaatg tgttaatgaa taaaccagat
    49921 tgatcccgta attatattac gccgcaaaat ccttacaata aacaggtttt tattgagcaa
    49981 atttgcattt ttcgacagtg atcaatttat agcgaaattg tcacgatctc accaatttga
    50041 tctacaacaa tttatcgtgt t
//
LOCUS       DAZFRE010000026        49795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFRE010000026 DAZFRE010000000
VERSION     DAZFRE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.026"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            107..739
                     /gene="comR"
                     /locus_tag="ACHKUI_001796"
     CDS             107..739
                     /gene="comR"
                     /locus_tag="ACHKUI_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6597997.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVASALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(801..1340)
                     /gene="ycfJ"
                     /locus_tag="ACHKUI_001797"
     CDS             complement(801..1340)
                     /gene="ycfJ"
                     /locus_tag="ACHKUI_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6597998.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(1567..2871)
                     /gene="ndh"
                     /locus_tag="ACHKUI_001798"
     CDS             complement(1567..2871)
                     /gene="ndh"
                     /locus_tag="ACHKUI_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6597999.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3271..3813)
                     /gene="ycfP"
                     /locus_tag="ACHKUI_001799"
     CDS             complement(3271..3813)
                     /gene="ycfP"
                     /locus_tag="ACHKUI_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6598000.1"
                     /translation="MIIYLHGFDSNCPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3836..4861)
                     /gene="nagZ"
                     /locus_tag="ACHKUI_001800"
     CDS             complement(3836..4861)
                     /gene="nagZ"
                     /locus_tag="ACHKUI_001800"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6598001.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4872..5696)
                     /gene="thiK"
                     /locus_tag="ACHKUI_001801"
     CDS             complement(4872..5696)
                     /gene="thiK"
                     /locus_tag="ACHKUI_001801"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6598002.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5677..6318)
                     /gene="lpoB"
                     /locus_tag="ACHKUI_001802"
     CDS             complement(5677..6318)
                     /gene="lpoB"
                     /locus_tag="ACHKUI_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6598003.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6332..6709)
                     /gene="ycfL"
                     /locus_tag="ACHKUI_001803"
     CDS             complement(6332..6709)
                     /gene="ycfL"
                     /locus_tag="ACHKUI_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6598004.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6712..7071)
                     /gene="hinT"
                     /locus_tag="ACHKUI_001804"
     CDS             complement(6712..7071)
                     /gene="hinT"
                     /locus_tag="ACHKUI_001804"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6598005.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            7406..9595
                     /gene="fhuE"
                     /locus_tag="ACHKUI_001805"
     CDS             7406..9595
                     /gene="fhuE"
                     /locus_tag="ACHKUI_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO6598006.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSEGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQNDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTFKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9655..11088)
                     /gene="ptsG"
                     /locus_tag="ACHKUI_001806"
     CDS             complement(9655..11088)
                     /gene="ptsG"
                     /locus_tag="ACHKUI_001806"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6598007.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11383..12180)
                     /gene="ycfH"
                     /locus_tag="ACHKUI_001807"
     CDS             complement(11383..12180)
                     /gene="ycfH"
                     /locus_tag="ACHKUI_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6598008.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12191..13195)
                     /gene="holB"
                     /locus_tag="ACHKUI_001808"
     CDS             complement(12191..13195)
                     /gene="holB"
                     /locus_tag="ACHKUI_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6598009.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVIWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13192..13833)
                     /gene="tmk"
                     /locus_tag="ACHKUI_001809"
     CDS             complement(13192..13833)
                     /gene="tmk"
                     /locus_tag="ACHKUI_001809"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6598010.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13823..14845)
                     /gene="yceG"
                     /locus_tag="ACHKUI_001810"
     CDS             complement(13823..14845)
                     /gene="yceG"
                     /locus_tag="ACHKUI_001810"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6598011.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14848..15657)
                     /gene="pabC"
                     /locus_tag="ACHKUI_001811"
     CDS             complement(14848..15657)
                     /gene="pabC"
                     /locus_tag="ACHKUI_001811"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6598012.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15777..17018)
                     /gene="fabF"
                     /locus_tag="ACHKUI_001812"
     CDS             complement(15777..17018)
                     /gene="fabF"
                     /locus_tag="ACHKUI_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6598013.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17106..17342)
                     /gene="acpP"
                     /locus_tag="ACHKUI_001813"
     CDS             complement(17106..17342)
                     /gene="acpP"
                     /locus_tag="ACHKUI_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6598014.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17553..18287)
                     /gene="fabG"
                     /locus_tag="ACHKUI_001814"
     CDS             complement(17553..18287)
                     /gene="fabG"
                     /locus_tag="ACHKUI_001814"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6598015.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18300..19229)
                     /gene="fabD"
                     /locus_tag="ACHKUI_001815"
     CDS             complement(18300..19229)
                     /gene="fabD"
                     /locus_tag="ACHKUI_001815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6598016.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19245..20198)
                     /gene="fabH"
                     /locus_tag="ACHKUI_001816"
     CDS             complement(19245..20198)
                     /gene="fabH"
                     /locus_tag="ACHKUI_001816"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6598017.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLATSEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20266..21336)
                     /gene="plsX"
                     /locus_tag="ACHKUI_001817"
     CDS             complement(20266..21336)
                     /gene="plsX"
                     /locus_tag="ACHKUI_001817"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6598018.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVISSDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21417..21590)
                     /gene="rpmF"
                     /locus_tag="ACHKUI_001818"
     CDS             complement(21417..21590)
                     /gene="rpmF"
                     /locus_tag="ACHKUI_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6598019.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21642..22163)
                     /gene="yceD"
                     /locus_tag="ACHKUI_001819"
     CDS             complement(21642..22163)
                     /gene="yceD"
                     /locus_tag="ACHKUI_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6598020.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22362..22946
                     /gene="yceF"
                     /locus_tag="ACHKUI_001820"
     CDS             22362..22946
                     /gene="yceF"
                     /locus_tag="ACHKUI_001820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6598021.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23058..24017)
                     /gene="rluC"
                     /locus_tag="ACHKUI_001821"
     CDS             complement(23058..24017)
                     /gene="rluC"
                     /locus_tag="ACHKUI_001821"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6598022.1"
                     /translation="MKTETPSVKIVAITADDAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24590..27775
                     /gene="rne"
                     /locus_tag="ACHKUI_001822"
     CDS             24590..27775
                     /gene="rne"
                     /locus_tag="ACHKUI_001822"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6598023.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSTPVVEEVAEVVEAPVQVTEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27970..28923)
                     /gene="flgL"
                     /locus_tag="ACHKUI_001823"
     CDS             complement(27970..28923)
                     /gene="flgL"
                     /locus_tag="ACHKUI_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6598024.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVDGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28935..30578)
                     /gene="flgK"
                     /locus_tag="ACHKUI_001824"
     CDS             complement(28935..30578)
                     /gene="flgK"
                     /locus_tag="ACHKUI_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6598025.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSTADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNNKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30644..31585)
                     /gene="flgJ"
                     /locus_tag="ACHKUI_001825"
     CDS             complement(30644..31585)
                     /gene="flgJ"
                     /locus_tag="ACHKUI_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6598026.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31585..32682)
                     /gene="flgI"
                     /locus_tag="ACHKUI_001826"
     CDS             complement(31585..32682)
                     /gene="flgI"
                     /locus_tag="ACHKUI_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6598027.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32694..33392)
                     /gene="flgH"
                     /locus_tag="ACHKUI_001827"
     CDS             complement(32694..33392)
                     /gene="flgH"
                     /locus_tag="ACHKUI_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6598028.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPAPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33445..34227)
                     /gene="flgG"
                     /locus_tag="ACHKUI_001828"
     CDS             complement(33445..34227)
                     /gene="flgG"
                     /locus_tag="ACHKUI_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6598029.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34399..35154)
                     /gene="flgF"
                     /locus_tag="ACHKUI_001829"
     CDS             complement(34399..35154)
                     /gene="flgF"
                     /locus_tag="ACHKUI_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6598030.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDDIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35174..36379)
                     /gene="flgE"
                     /locus_tag="ACHKUI_001830"
     CDS             complement(35174..36379)
                     /gene="flgE"
                     /locus_tag="ACHKUI_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6598031.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36404..37099)
                     /gene="flgD"
                     /locus_tag="ACHKUI_001831"
     CDS             complement(36404..37099)
                     /gene="flgD"
                     /locus_tag="ACHKUI_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6598032.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGIGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTATNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37111..37515)
                     /gene="flgC"
                     /locus_tag="ACHKUI_001832"
     CDS             complement(37111..37515)
                     /gene="flgC"
                     /locus_tag="ACHKUI_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6598033.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37519..37935)
                     /gene="flgB"
                     /locus_tag="ACHKUI_001833"
     CDS             complement(37519..37935)
                     /gene="flgB"
                     /locus_tag="ACHKUI_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6598034.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38090..38749
                     /gene="flgA"
                     /locus_tag="ACHKUI_001834"
     CDS             38090..38749
                     /gene="flgA"
                     /locus_tag="ACHKUI_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6598035.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RIKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38825..39118
                     /gene="flgM"
                     /locus_tag="ACHKUI_001835"
     CDS             38825..39118
                     /gene="flgM"
                     /locus_tag="ACHKUI_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6598036.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39123..39539
                     /gene="flgN"
                     /locus_tag="ACHKUI_001836"
     CDS             39123..39539
                     /gene="flgN"
                     /locus_tag="ACHKUI_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6598037.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39579..41114)
                     /gene="murJ"
                     /locus_tag="ACHKUI_001837"
     CDS             complement(39579..41114)
                     /gene="murJ"
                     /locus_tag="ACHKUI_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6598038.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41224..42147)
                     /gene="yceM"
                     /locus_tag="ACHKUI_001838"
     CDS             complement(41224..42147)
                     /gene="yceM"
                     /locus_tag="ACHKUI_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6598039.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFLAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42149..42796)
                     /gene="yceH"
                     /locus_tag="ACHKUI_001839"
     CDS             complement(42149..42796)
                     /gene="yceH"
                     /locus_tag="ACHKUI_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6598040.1"
                     /translation="MKYQLTAMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLASREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMLNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42807..43391)
                     /gene="rimJ"
                     /locus_tag="ACHKUI_001840"
     CDS             complement(42807..43391)
                     /gene="rimJ"
                     /locus_tag="ACHKUI_001840"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6598041.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMISEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43627..44835
                     /gene="mdtH"
                     /locus_tag="ACHKUI_001841"
     CDS             43627..44835
                     /gene="mdtH"
                     /locus_tag="ACHKUI_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6598042.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKCFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44899..45546
                     /gene="grxB"
                     /locus_tag="ACHKUI_001842"
     CDS             44899..45546
                     /gene="grxB"
                     /locus_tag="ACHKUI_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6598043.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45680..46240
                     /gene="yceB"
                     /locus_tag="ACHKUI_001843"
     CDS             45680..46240
                     /gene="yceB"
                     /locus_tag="ACHKUI_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6598044.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46346..47392
                     /gene="pyrC"
                     /locus_tag="ACHKUI_001844"
     CDS             46346..47392
                     /gene="pyrC"
                     /locus_tag="ACHKUI_001844"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6598045.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47466..47711
                     /gene="dinI"
                     /locus_tag="ACHKUI_001845"
     CDS             47466..47711
                     /gene="dinI"
                     /locus_tag="ACHKUI_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6598046.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48001..48255
                     /gene="bssS"
                     /locus_tag="ACHKUI_001846"
     CDS             48001..48255
                     /gene="bssS"
                     /locus_tag="ACHKUI_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6598047.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48370..49488
                     /gene="solA"
                     /locus_tag="ACHKUI_001847"
     CDS             48370..49488
                     /gene="solA"
                     /locus_tag="ACHKUI_001847"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6598048.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPAHDNTLLITG
                     LSGHGFKFASVLGEMAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49536..49649
                     /locus_tag="ACHKUI_001848"
     CDS             49536..49649
                     /locus_tag="ACHKUI_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6598049.1"
                     /translation="MRRLVNYLINNIREHLMLYLFLWGLLAIMDLNYVFYF"
BASE COUNT        12106 a        13397 c        12311 g        11981 t
ORIGIN      
        1 ttcagttaaa tagaaagcaa ttatttttat ggaatctgga ctggtgaacg gaggtgatta
       61 tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc
      121 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
      181 tgataaggcc atgaaattgt tctggcagca cggttatgaa gcgacttctc ttgcggacct
      241 cgttgaagcg accggagcta aagcgcccac gctatacgcg gaatttacca acaaagaggg
      301 gttattccgc gccgtactcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
      361 gctgttttgt gaagagaaaa gcgtggcgtc tgcgctggct gactattttg ctgccatcgc
      421 caactgcttt accagcaaag acaccccggc gggctgcttc atgatcaaca actgcaccac
      481 cctctccccg gattctggag atatcgccaa tacgctgaaa tcacgccatg cgatgcaaga
      541 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
      601 tgacgtgacg catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
      661 acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc cgaacgactt tgcgtttatg
      721 gccggaactg ctgaaataag tcgcaaaaaa agcccctcag cttgagcgaa tgaggggcta
      781 aattgcagag tacaaccttg ttatgctttg ttattcaaaa tcagctgccc gttgctatct
      841 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
      901 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
      961 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttacctgca
     1021 tatccacccc ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accaccaaac
     1081 tgatgcccaa tcacgccgcc agcaacagcg ccgagcaccg acccggtaat gcgattttca
     1141 tcctgcaccg gtcgacgatg ggtcactgta acgttgcgac actcctgacg cggtgtttta
     1201 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc acgttcaaac
     1261 acgttcagac tggccactgc cgctacgccc agcgcagctg cgacaccaat cccgataccc
     1321 gccaacattg acttattcac gggaatacct ccttctttgc tgttatgcca atcatgcaag
     1381 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatggc tggaaatact
     1441 tagggattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
     1501 ctaccgctcg taccatttgt ccagggcgga taaggcgttt acgccgcatc cgccagtaca
     1561 agccgattaa tgcaacttca aacgtggacg gataacacgg ttaatactcc ccaccagcat
     1621 cattaatccg gttttaaagt aaccatgcag cgcaatctgg tgcattcggt acagcgagat
     1681 gtatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat
     1741 caggctaccg acggtggaaa agttcgacag cgataccagc gaaccgtgat ctttatactg
     1801 ataatttttc agcggcttac cgttcatctg cgccagaatg ttgttcattg cacaggttgc
     1861 catctgatgt gcggcctgag cgcgcggcgg aacaaagccc ccttccggac gcgggcatga
     1921 cgcgcagtcg ccaatagcgt atatgtctgg atcgcgagtg gtttgcagcg tcggttccac
     1981 caccagctgg ttgatacggt tggtttcaag accaccgata tctttcagga agtctggcgc
     2041 tttgatcccg gctgcccaca ccatcagatc agcctcaata tattcgccat ctttagtgtg
     2101 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgcctagttt
     2161 cgttaactca ctatgggccg cagcagagat acgtggcggc aatgcaggca aaatacgttc
     2221 gcccgcttct accagcgtta cgttcagggc ttcgttggtc agacctttat aaccgtaact
     2281 gtgcagttgc ttgaccgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc
     2341 gccgacaatc gcaatgttta ctttgccatt cgcgcccagg ttggcggagt atttcaggaa
     2401 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
     2461 gttctctttg acacctggtg tattgaaatc gttagaggtg ctacccagcg ccattaccag
     2521 ggtgtcatag gcgattttac gttccggaac cagcagttca cctttctcgt cgcgcagttc
     2581 tgcaatagtg attgttttcg cttcacgatc aatatcaatt acggaaccca gctggaactg
     2641 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
     2701 gccagtcgcc acttcgtgca gcagcggttt ccacaggtgg ctgtggttac gatcgaccag
     2761 cgtaattttg gcttttttct tgcgtcccag cttatgcccc agctgtgttg ccatttccag
     2821 cccaccagca ccgccgccga caatcacaat ttttttcaat ggcgtagtca acgtgacccc
     2881 cttaaattta ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca
     2941 ggttacagca atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat
     3001 accaaaattg atgttaatca agttttgttg ctgaaaagat aggcgacttt accgggtgag
     3061 atcaaaaaac agagtttgtt gtttattatt tgagcgaact tgtgagagtg atatagagtg
     3121 aggggtgtat ggagggcatt cggtaggcct gataaaaggc gcatcaggca ttgatgctcg
     3181 actgtcggat gcggcgtgaa cgccttatcc aaaccacaga atccggagcg catcaggtac
     3241 cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
     3301 ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
     3361 gtaataatga tgcaactctt cagatgtccg ctggctgtta agcgcttcat cattacgcga
     3421 caaaatcacc aggcaacgat cgcgattctt ctcacggaag ttggtcacac acttagtggc
     3481 aatatcggca tactcttccg ggcgatcaat cttcccttcc atgttctcat aagggaacaa
     3541 attggggttg aagatcacct ggcggatatc acagagaaaa ccaatccgct cagcccaata
     3601 accgcccaga ccaacaccac aaatgagcgg gcgctcgtcg acgttcagtt gcaacatttt
     3661 gtccacttct ttgagcaaat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
     3721 tagccgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggaca
     3781 gttagagtca aaaccgtgca aatagataat catcgcttcc tcacataagc cagggttaat
     3841 gacctgcctt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt
     3901 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
     3961 taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
     4021 tattgcagac caggatcata tcgcaacctg catccagtga tgcctgtcca cgctcggcat
     4081 aactgcccat aatcgctgcg ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa
     4141 aacccaattc ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg
     4201 gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
     4261 ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgcgga cgtgggtcgc
     4321 acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg acctgggaag tgtttcccgg
     4381 tagttttcat tcccgcttca tgcataccat caataaaacg gctggcaatt gccagggctt
     4441 tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacgtcca
     4501 gcactggcgc aaagctgata tcaatatcca tagcaatcat ttcactggcc atcaaccaac
     4561 ctgcctcttg cgccagtttt ccgccttctt ccattcccaa cagcgcagcg aatgattgtg
     4621 ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac gcgtccacct tcctgatcta
     4681 ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact
     4741 gggcaggatc atgatagttt cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca
     4801 gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca
     4861 cactgctctc cttattgttt tattaatagc tgccgccacg tgtcatcggc cagcctgata
     4921 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctgcttt gagcatcagc
     4981 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
     5041 gtggcatagt cattgaccaa ttgccgatgc tggtcaatat tttccaccca caccgccgcc
     5101 agttccagcg cgatatcacc atctccggca tactcccagt cgatgagttt taaccctgac
     5161 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggc
     5221 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac tgtccgccgc
     5281 gccggatcgc tttgctgcca gtacagttcc agtaacggca acagcgttat tcgccagcca
     5341 aaacgcggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
     5401 tacgttttta cctcgccggg cagatagtcg accaccatcc agtcacggag atataaatgc
     5461 ggcttcggtg caatgcatgc gggtagttgt gataaagccc gatactggcg caagaacgcg
     5521 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca
     5581 atgagaaaac tcccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
     5641 aaaaagcgcg acagcaattc gtcgcgcgtg aggggattat tgctgcgaaa cggcaccttt
     5701 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
     5761 gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccaacat tgcgggcaat
     5821 gcctatggct ttactacggg tgcctaaact gtcctgcggc gataaaccta attgttgctt
     5881 agccattgac agctgctggg cggaaaccag ggtaaattta ccgttattag ccagcgcatt
     5941 tcgcagcgtt tcggtcgctt ctgcggcatt caacgaaccg ttagtacggt tgttaacgct
     6001 atcaaccagc aggacgctac ccgcagtcac gccgtcagcc ccaagcatct tactgaccat
     6061 cggctgcatt gcgccattcc agtcataatg acgaatatgc ggcgcaggcg gtgcagtttg
     6121 atcttcgtgc tcaattgggc ctggctgctg cggtatcgtc ggcaccgatg gcactgtggg
     6181 gacaggctgt tgtggctcag caggttgttc cggcgctggt ttcacttctt ctaccggtgc
     6241 aggttcacgt tgccccacac acccggcaag aaacatcgcc agcgcggtaa tcaaggcgta
     6301 gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt ctgactttgt
     6361 gcgcccccag aaaattggcg ctgccgtaca gcgtgaccgc cgaatgtgcg ggaatggtaa
     6421 cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac
     6481 gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt
     6541 gaatatcaga tgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg
     6601 actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca
     6661 ccagcactaa caacgccaga gacatcagcc caaagcatcc ttttctcatc gttacagacc
     6721 tttatgcgtc agcattggtc ccagcggacg gccacccaac aaatgcatat ggatgtggta
     6781 aacctcttgt ccgccatggc ggttggtgtt catgatcaga cgatagccat cttcggcaat
     6841 accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg
     6901 ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt
     6961 tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc
     7021 ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc
     7081 ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt
     7141 atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt
     7201 tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca
     7261 ttctctgttt ggtttacact ccaaatatga atgcgtatat ttctcatttg catttacaca
     7321 caaaaattat tcgcacacta aatataaccg ctcatcaccc tgcttcggca tgagcctgac
     7381 gccaacataa accaagagat ttcaaatgct ttcgacacaa tttaacaggg ataatcaaca
     7441 tcaagccatc atcaaaccgt cactccttgc cggttgcata gcactggcac tattaccttc
     7501 cgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc
     7561 cccagatgat ggcgaaaatg attacaacgt aacgtctacc tctgagggta caaaaatgca
     7621 gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga
     7681 tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca
     7741 ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt
     7801 tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat
     7861 ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggcctcatga ccgggacggg
     7921 taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg
     7981 cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag
     8041 cccactcacc gaagacggta aaatccgcgc acgaattgtc ggcggctacc agaataacga
     8101 ctcatggctg gaccgctaca acagtgaaaa gaccttcttc tccggcattg tcgatgctga
     8161 tttaggcgat cttacgatgc tttcagccgg ttacgaatat cagcgtattg atgttaatag
     8221 cccaacctgg ggtggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg
     8281 cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat
     8341 gaccctgaag cagcggtttg ctgatacctg gcaagcgacg ctgaatgcca cccactctga
     8401 agtcgaattt gacagcaaaa tgatgtatgt cgatgcctac gtaaacaaag cggatggtat
     8461 gctggttggg ccatacagca attatggacc tggctttgat tatgtcggcg gcaccggttg
     8521 gaacagcggc aaacgtaaag ttgatgcgct ggatctgttc gctgacggta gttatgaatt
     8581 gtttggtcgt cagcacaatt taatgtttgg tggcagttac agcaaacaaa acaatcgtta
     8641 cttcagttca tgggccaaca ttttcccgga tgaaattggc agtttctaca actttaatgg
     8701 caatttccca caaaccgact ggtcaccaca gagcctggcg cagaacgata ccacacatat
     8761 gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg
     8821 cgcacgttat accaactggc gagttgatac gctgacttac agcatggaga aaaaccacac
     8881 cacgccttat gctggactgg tgtttgacat caatgacaac tggtcgacct acgccagcta
     8941 tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tgactccaat
     9001 caccggtaac aactacgagc taggtctgaa atcggactgg atgaatagcc gtctgaccac
     9061 cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat
     9121 ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggtgt
     9181 ggaatttgaa ctcaacggcg caattaccga caactggcag ttgacgtttg gcgcaacgcg
     9241 ctatattgcc gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacgtt
     9301 taaaatgttc accagctatc ggttgcctgt catgccagag ttgacggtcg gcggtggtgt
     9361 taactggcaa aatcgcgtgt ataccgacac cgtgacgccg tatggcacct tccgcgccga
     9421 gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc
     9481 gttacagggg aatgtcaata acctgttcga caaaacctac gataccaacg tggaaggttc
     9541 tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac
     9601 acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg
     9661 gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc
     9721 ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg
     9781 atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt
     9841 agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc
     9901 tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt
     9961 aatcagcacg cggaagatgg tgtagtaaac aatggcataa ccgataccga cgatcgggaa
    10021 cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa
    10081 cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat
    10141 cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga tcggctcggt
    10201 gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg
    10261 gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt
    10321 gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat
    10381 gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg
    10441 tacgttccag atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat
    10501 gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga
    10561 accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag ccaggcctga
    10621 aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg
    10681 gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccccc cgagtacgcc
    10741 agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc
    10801 aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac
    10861 gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc
    10921 aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa
    10981 attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata ccggcagcat
    11041 cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc
    11101 tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag tgcctcgccg
    11161 tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt cagtgccgat
    11221 ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa
    11281 aacatatgtc acaatatttc aaacgtggtt attcaccgcc cgattttcgg cggtgaactt
    11341 tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag
    11401 gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc
    11461 ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg
    11521 ttgattctct tttcctcgat gcggtactgg cgcaaggtaa ggtgagtcag tttccaccag
    11581 taaccgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt
    11641 cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttgcccg ccgtttctct
    11701 gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat
    11761 tgccagcgta tcggcgcggg cgtcacgggt gtggacgata accggcttgt tcagttcacg
    11821 accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt ccggcgtgta
    11881 ataataatcc agcccggttt cacccagcgc tacaacgccc tcttctgccg ccagacgacg
    11941 taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac
    12001 gacgttgtcg cgttcgccta ccagatcccg catgtgtaaa taacccggta atgttgtggc
    12061 gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc
    12121 cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat
    12181 gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa
    12241 tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact
    12301 gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt
    12361 ttgccagttc gacgaccagg cccggcacat caacattggt cacctgcgca gcgccatgat
    12421 ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgtgcc ggagcttgtt
    12481 catgattaag ggccgctagc agcgaatacc agtcgcccga ttgcacgcta tatgccagcg
    12541 cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgccc
    12601 caggcgaacc ggcgcttaag cgcaatgcgg caagtagtgc atcctgtgac attgtcactt
    12661 cgcgtgaaag ccagatcacg gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac
    12721 aacgactacg taatgttgcc agtaaacgtt ctggctcgcg ggtagcaagg aaaaaccacg
    12781 tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggttagta
    12841 aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcatgc tcattcagct
    12901 tttcggtgac ctcacgtacc gcatcaatgc ccagcgcatt tttccctttt tcgggagcca
    12961 gggtgtagta atcgggatgt gtgccagcct gcatcaactg acatccacga cagtgaccgc
    13021 aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag
    13081 catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct
    13141 gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc
    13201 caactccttc acccagttgg tcacggtagt gcggattgca tccatcacgg cctccagcgg
    13261 ctgggtggca tcaatggtat gaatgctttt atcttgtgct gccagttcca gatagcgggc
    13321 gcgggtgcga ttaaagaaat cgaatgactc ttgctcaata cgatccagct caccacgggc
    13381 gcgagcacgt tttaagccga cctctggggt gacatcaaga taaagcgtta agtcagggcg
    13441 aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa tacctcgtcc
    13501 tccaccctga taggcttgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt
    13561 agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag
    13621 aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct
    13681 tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat
    13741 acccagttgc tcaagcgtct caaccaccac gttacgcgcg gtagttttgc ctgcgccttc
    13801 cagcccctca atgacgatat acttactgcg catttttttc cttaagcact ttcagataat
    13861 cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat
    13921 cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg
    13981 cccccggcgt cgctatcgca cctggcggca gaccggtaat ggtataggtg ttatacgctg
    14041 tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa
    14101 tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata aatactgagg
    14161 caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca
    14221 ccaactggtt tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga
    14281 ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg
    14341 tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa
    14401 gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt gtgcttgata tacggagcct
    14461 cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact
    14521 gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa
    14581 agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc aaccattgaa
    14641 aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcacctagc gccagacgtc
    14701 cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa
    14761 gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca
    14821 agattatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata
    14881 ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg gcatcactgg
    14941 cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca aagaggcttg
    15001 cacttcgaca agctgataag aggattgtgc cagcaaacgg atacagaatt gtcgcataat
    15061 gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttaccct tccgccagaa
    15121 caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc
    15181 agcgtttgtc tgctcaagat gagagcggat caatacttgc tcaagccgat taagatgttt
    15241 aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca acatcatccc
    15301 ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta atggagagaa tccgcgttgc
    15361 cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt
    15421 cagcacacca ttctgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca
    15481 gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga
    15541 taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg
    15601 cgttgcccga tcgcttactg ccagcgattc ctgcttatga ccgttaatta agaacatacc
    15661 ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata
    15721 aaagctaaga aaaaaggccc gcaagcggac cttttataag ggtggaaaat gacaacttag
    15781 atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagagtg
    15841 tattccattc cgctaacctg acgcgcttcg tgcggtacga aatccagatc gcaaccttcg
    15901 tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag
    15961 tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag
    16021 cttaccaaca cgcggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct
    16081 ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg acttgcttca
    16141 atgcctgcat cacgcagagc atttgccatc gccagagctg cgcctgcgcc attttctggc
    16201 ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt
    16261 ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg
    16321 cccagtacga aaccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca
    16381 ttgcgggtag acaatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct
    16441 ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca
    16501 tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc
    16561 aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag
    16621 aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt
    16681 ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt
    16741 atttcaaggc cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag
    16801 gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact
    16861 aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca
    16921 ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac
    16981 atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca gggagggaaa
    17041 aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg
    17101 ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc
    17161 tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca
    17221 acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct
    17281 tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc
    17341 atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg
    17401 ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc
    17461 cttacccccc aataacgcaa atcattttgc actaattatt actcataacc acgcagactg
    17521 cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca
    17581 tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg
    17641 gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc
    17701 tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt
    17761 acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc
    17821 gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg
    17881 atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga
    17941 cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc tttcattcgc
    18001 attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat
    18061 tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc
    18121 agacctttgc cgttggcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc
    18181 gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt
    18241 ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt
    18301 taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg
    18361 tcgacaatgc gtttcgtcag gccagtaagc actttgcccg ggccgacttc atagagatgt
    18421 tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac
    18481 tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta
    18541 ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg
    18601 tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagagcg
    18661 cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg
    18721 gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca
    18781 gcttcttcac acgcttttcc aatagacgca tcatccagac cgatgattgc cgccatagcg
    18841 cccgttcctt ccggtacggc ttcttgcatg aacttgccgc gcatctcaac cagacgcacc
    18901 gcatcagcga aatcaatcac accagcgcaa accagcgcgg agtattcccc caggctgtga
    18961 ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca
    19021 gatgcagtca acagcgctgg ctgagtttgc caggttttat tcagttcttc agctggcccc
    19081 tgctgggtca gcgcccacag atcgtagccc agcgccgcag aagcttctgc aaacgtttct
    19141 tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt
    19201 ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc
    19261 gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg tcccggctta
    19321 atgcgcccgt cacgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta
    19381 ccgtggcgat ccagcgtcac cacgacattg tccatcgaca tgccgagttt tttcgccgtt
    19441 gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca
    19501 agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg
    19561 aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac acggtcagca
    19621 ttaggcagcg tcagcaattc accatagcta ccatcggcat gcagatgggt ggagatgatt
    19681 cccggctctt cagaggtagc cagcaccgca gcgcccgcgc catcgccaaa aataataata
    19741 gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca
    19801 tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct
    19861 gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccaacat gctttgaatc
    19921 tgacaagctg cgctcgggaa agcgtgcgtc gcagaagtcg ttgccacaac gatcaagcca
    19981 atctggtctt tctcaatacc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg
    20041 gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca
    20101 atccactcgt cagaggtatc caccattttt tccaaatcgg cgtttgtccg cacttgttcg
    20161 ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt
    20221 tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc
    20281 gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc
    20341 aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa
    20401 agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca
    20461 ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag
    20521 ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag
    20581 cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat ttcctgtaaa
    20641 gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc
    20701 gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcgag
    20761 accctttact tcttcttcac caatattgag caacgccacg cgaggattag gaatttccac
    20821 cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg tactgtcaca
    20881 atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat gcggtaatac
    20941 cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc
    21001 catcagcgcc ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag
    21061 ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc
    21121 atcactggag ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc
    21181 agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg
    21241 cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc
    21301 tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt
    21361 ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac
    21421 ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt
    21481 ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa
    21541 cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact
    21601 cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg
    21661 gctaatacgg caaatgggtt tggcttttgc gcttcttcag gcagttcacc aaagaccatg
    21721 tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg
    21781 atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc
    21841 ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa
    21901 tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc
    21961 gtcaccttcg catcgccatt taacactgcg agacgttggt tatcgatagc gaacgacatg
    22021 gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca
    22081 ggagtataga taccctggta atcaaggcgt ttctgagccg tacgaaccgg atcgagagtc
    22141 aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca
    22201 aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg
    22261 ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta
    22321 aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag
    22381 cctccacctc gccctggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg
    22441 cagcacccga ggtcgacgaa actccacgca gcgacgaatc accgcgacag ttggtgcttc
    22501 gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg
    22561 gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa
    22621 atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg
    22681 cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt
    22741 tccgtcacct gagcgaggcg gagattgata attacgtgcg taaagagcat cccctgcact
    22801 gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagaggggc
    22861 gtgatcctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg
    22921 aagggaaaaa cccgctgatg ggatagtttt ttgcgccgga tgcggttact tcgcatccgg
    22981 caatcggtgc tggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc
    23041 aggtttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct
    23101 tcatccatcg gcgcttcaat acgcatcacc tcaccggtcc ccggatgagt aaacttcaac
    23161 gctgcggcgt gcaggaacag gcgatttaat cccgtgcctg cttcagtgag ctgtctgtca
    23221 aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata ttgtgtatgc
    23281 acacggatct gatgagtacg ccctgttacc gggctacaac gcaccagggt ggcaaatgca
    23341 tagcgctctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact cacacgcacg
    23401 atacgttcgc cgctttgcag aatatttttc agtaacggcg cttggacgct cttcacatgc
    23461 gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt ttcacgtaat
    23521 tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaggtg
    23581 tcccggtcaa gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa
    23641 ccttcaataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta
    23701 ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt
    23761 tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc
    23821 acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact
    23881 tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc
    23941 aaaaagttgt cgatacgttg ccccgcgtcg tcagcggtga tagcaacaat ttttacggat
    24001 ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt
    24061 tttctatgct tatatttagt tttacacctt attacttcac taggtgatca ctttattgat
    24121 ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg
    24181 gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc
    24241 atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg
    24301 cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc
    24361 ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc
    24421 gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata
    24481 accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt
    24541 actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat
    24601 gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct
    24661 gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg
    24721 taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg
    24781 tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc
    24841 tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat
    24901 cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg
    24961 tagctatctg gttctgatgc cgaacaaccc gcgcgcgggc ggtatttctc gtcgtatcga
    25021 aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat
    25081 ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc aatgggattt
    25141 aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc
    25201 gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt acttacgcca
    25261 ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac gtcagcatat
    25321 cgctgcatta ggccgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc
    25381 gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagttcg
    25441 tctgccatcc ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat
    25501 caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta acactaacct
    25561 cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt
    25621 tatcgacttc atcgacatga cgcctgtacg ccaccagcgt gcggtagaaa accgtctgcg
    25681 tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct
    25741 gttggaaatg tcccgtcagc gcctgagccc atcactgggt gaatccagcc atcacgtctg
    25801 cccgcgctgc tccggtaccg gtaccgttcg tgacaacgaa tcgctgtcgc tctctattct
    25861 gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc
    25921 tgtgccaatt gcttcttacc tgctgaatga aaaacgttct gcggtaaatg cgattgaaac
    25981 tcgtcaggac ggcgtacgct gtgtgattgt gccaaacgat cagatggaaa ccccgcacta
    26041 ccatgtgctg cgcgtgcgca aaggggaaga aaccccaacc ttaagctaca tgctgccgaa
    26101 gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc
    26161 ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcac caacgccagc
    26221 tgaacctgcc gcgcctgtcg tagccccagc acctaaatct gcaccggcaa caccagccgc
    26281 tcctgcccaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg
    26341 tggtgaagaa accaaaccgt ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg
    26401 tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga
    26461 ccctcgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg
    26521 ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggcag aggtaacgga
    26581 aaaagcgcgt accaccgacg agcagcaaac gccgcgtcgt gaacgtagcc gccgccgtaa
    26641 cgatgataaa cgtcaggcgc aacaagaagt gaaggcgctg aatgttgaag agcaatctgt
    26701 tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca
    26761 gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt
    26821 ggttgaagaa actgtcgcag ccgaaccaat tgttcaggaa gcggcagccc cacgcacaga
    26881 attggtgaaa gttccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa
    26941 caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca
    27001 cctgcgcgta agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc
    27061 gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat
    27121 ccgctatcca attgtacgtc cgcaagatgt acaggttgaa gagcagcgcg aacaggaaga
    27181 agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgccg aacctgttgt
    27241 tagcacgcca gttgttgaag aagtggctga agtcgtagaa gcccccgttc aggttaccga
    27301 accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgctgccg cggtaactga
    27361 acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg cagaacacgc
    27421 agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc
    27481 tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga
    27541 agtcacagca gaagttgaaa cggtagctgc ggttgaacct gaggtcaccg ttgagcataa
    27601 ccacgctacc gcgccgatga cgcgcgctcc ggcaccggaa tatgttccgg aagcaccgcg
    27661 tcacagtgac tggcagcgcc ctactttcgc cttcgaaggt aaaggtgccg caggtggtca
    27721 tacggcaacg catcatgcat ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    27781 agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcatct ttgagtcaac
    27841 atattaaaat tcatcaaatg gcatccttgc taaccaacaa tacaaaatag gcaaaaaaat
    27901 gcccgccccg gcgatcccgg agcgggcaga caaaacatac ccagtttcat gatatgtttt
    27961 aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt
    28021 atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc
    28081 cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaatga
    28141 atccagcgac tccagttcgt tcagctgcgt gcctaattcc gcgcgaacag tcagcacatt
    28201 gttcagcgag tttttcagtc cgcggttggt tttatccagt gccgcagcgg cggtttcttt
    28261 atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc
    28321 aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttgc tggtaatact
    28381 gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg
    28441 cttaatactt tctgttccgc cgacataatc accgtcaacc tcactaaacg gcgcagtctc
    28501 tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg ccagattcag
    28561 caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa
    28621 ggtgccattg ctggcgtaaa caattttttc ctgggcattc tggatagcag tggtgacctg
    28681 gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgcg ccagcgtgta
    28741 ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc
    28801 atctgaaggg ttaacgactc gcttacccgt cgacatctgt tcgccgtact tcatccattc
    28861 tgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac atcatctggg tactgaaacg
    28921 cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc
    28981 agaacctgcg cattcgcgag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca
    29041 tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct
    29101 tgcgtggcgc tactggtttt caacgtcgcg gttttattcc cgatatcact cactaatgag
    29161 gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tattgttgct ttgcagatcc
    29221 agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttctgcgct cgccatggcg
    29281 attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact taccggtttc
    29341 agtgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat
    29401 gccactttac cgttggcatc cggcgtcacc gtaaaagtgg tattactggc aaggcgggtg
    29461 acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac cgcggaggca
    29521 tcagttaccg tggcaccgat cgcaacgtca ccgttgtttt tcgtgttttg cagtaccgcg
    29581 ggcttaccga tagcaaagaa atcttcaccg gcatcgccgt tagcatcaaa tccggctttg
    29641 tgttgggtgt tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc
    29701 tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat
    29761 ttctccggga tctcaatatt gccagccgtc ccatcaatat aagcgacagt cgtacgcgaa
    29821 gggtcggcgg tggaaggaac tgccgccagc tgccgcgccg tacttccctg aaccagtgag
    29881 tagccattgg ccatcgtgat gttataagtg ccaccatcct gaacgctaac ttctacgcca
    29941 acaatctggt ttaattcact caccagttga tcgcgttgat ccagcagatt gttaggtgac
    30001 gcccctgccc ccacgcctgt cagacgcgag atttgatcgt tcaggctggc gatttgttta
    30061 gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg
    30121 tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc
    30181 agcgcctggc gcgctgccgg gtcttccgcg ttacttacca gcgtttgcag gctggtgaag
    30241 aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc
    30301 gacatctgct catagcgggc agtcaggccg ctactttgcg tctgcgccgc acgtaactgg
    30361 ttggtaataa atgcatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc
    30421 cagccgccag cgcccagcgt gctattggcc tgcgccataa tagtggtttg gcgggtatat
    30481 ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg
    30541 ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca
    30601 attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa
    30661 tgttcatact gtaggttttg ctcactttgt cgcttatcga tttcatctgc tgaatcatgt
    30721 tggtgagttt gcgggcatag tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg
    30781 ccccctgctc cgcactcgcg gcggtcgtca cggcagcgta gcgcggatta cgtgttaaca
    30841 gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaat ttcgctttta
    30901 ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact ggccctttcc
    30961 agttgccaga ggctttgacg ccaaacaggt tataactcgg ctcgccgttt tcacggcgga
    31021 tttgccgttg tccccagcca gattccagcg ccgcctgagc gaggatcaaa tgatgtggca
    31081 caccgctttg ctggcttgcc agttgggcgg gcaacgagag ttgcgcgagg aatgctttac
    31141 tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa
    31201 gcgtctgatt ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg
    31261 tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc
    31321 ccagaccttt gcccgccgtc atctgttggg caatctgctg gtcatacata ctggtataca
    31381 ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca
    31441 acatcatctg cacgaacatc ccttccacct ggcgggccac cggacggata tttgccgctg
    31501 gatcttcgcc cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg
    31561 ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc
    31621 actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag
    31681 cgcgcgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct
    31741 ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc tgtccaccac caaacggtgt
    31801 atctggctgg ctgacattgg cctgacgatt gaccgtaacc gagagattcc cctgcgctac
    31861 tgcgcagctg tccagggtca cttcgcgatt catcaccacc gagccagtgc gcgagttaat
    31921 cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa
    31981 acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc
    32041 ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct
    32101 gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa
    32161 ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt tcacctgaac
    32221 actgctaccg ccagcggagg ctccagcgcc accaaccaga atattgccct gcgccagcgc
    32281 atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac aacgtaccgc cacgcaagct
    32341 tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctggc gtccaaacgg
    32401 cggaagtgac gctgtcacca ttaccgcagc gacgtttttt agctgcatat tggtgcccgt
    32461 cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt
    32521 ctgggttgtc tggtcaccgg ttccatccag ccccaccacc aggccatagc caatcagtga
    32581 gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt
    32641 gataaccagt agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt
    32701 ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag
    32761 ccattgccta cgtattcaat gcgcgcatcc gccacctgag tagatggtac ggtattgctg
    32821 ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa taaattcggt accctgatta
    32881 atggcgatct gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc
    32941 gtcaccgtca acgtaccgct aaaggtattg ctggcattgg ccccgccttt tccgttgaac
    33001 gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacaaccc ctgcaaatag
    33061 cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag
    33121 cttttgctgg cgctgacgtt ctcctgcaac acgatagtca gcgtatcgcc aatattgcgt
    33181 ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa
    33241 atagacccgt tggcgacggg cgtcggaccg ggagccggtt gtgcactggt cgccccttgt
    33301 accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa
    33361 atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc
    33421 ggtgaacctg ccaccggtta agccttagag ttgcgtcagt ttttgcagca tctgatcggt
    33481 ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag
    33541 ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc
    33601 gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt
    33661 ttcgccaatg ctctccagtc cggtgtcatt cataaaggtg gtgagattga gctgcccaac
    33721 ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt
    33781 gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccggccgt
    33841 caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc
    33901 atctggcaac atcacctgga aaaagccctg ccctttaatc gcgacgtctt tactgttatt
    33961 ggtttgcgac aggttgccct ggctgtgtaa acgttccgtg gcgaccgggc gtacccccgt
    34021 gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg
    34081 aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact
    34141 gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt
    34201 tttggcgatc cataatgaac tgatcataaa atgtcctgta gtggattagt gacaaagtgc
    34261 atatgtcttc ttgccggatg cggcgtaaac gccttatccg gcctacaagt gctcgcaaaa
    34321 tcgataaatt gcgattgccc ccatagaccc gataaacttg cgcatagggc aacttatact
    34381 tgtcatgtat cctttcaatt aactcatcga cagcagttgg ttggcacggc ctgcgttatc
    34441 atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc
    34501 gctcattgcc gcaacggcat tgacgttact gccttccaga acacccgaca tcacacgcaa
    34561 ggttggatct gcctgcagta ccggcccacg cgtagcctgg ctttctgcgc ttaaacgaaa
    34621 aatgtcatcg tcaccgcgct ggacttcgct gcccgtggct ttcaccagct tcagacgccc
    34681 cactggcgca accgtatttg ccggatcgcc cggattcagc gccgagattg tgccatcggc
    34741 agcaatagtg atttccgccc cttccggcac agcaattggc cccgcctcgc ctaccaccgg
    34801 atgtccctga attgtcagtt gcccggtggg atcaacctga atgctgccat tacgcgtata
    34861 cccttcacta ccgtcagcgg tctgcacgac cagccaacca tcctgctgca acgcaacgtc
    34921 cagcgggcgc gaggtgtaat ccatcttgcc gggcgtcatg tctgcacccg gtgttgacgc
    34981 cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc ggcactgcgc gcaaggcgtt
    35041 taactgcgcg cgaaaaccgg gcgttgaggc attggccaga ttactggcgg ttaccgcctg
    35101 ttgattcagc gtctggctgg ctgctcccat cgcggtataa attgcgtgat ccattgagct
    35161 atcccgtcag cgattagcgt aagttaacca gcgtattgag gatctggtcc tgggttttga
    35221 tggtctgggc gttagactga tagttacgct gggcaacgat catattgacc agttctttac
    35281 tgagatcgac gttggacgct tccagcgcac cgttggtcag tgtgccaaag ttgcccgtcc
    35341 cggctgtccc caacagcgcc acgccagaag attgcgtagc ggaccagacg ttgtcgcctt
    35401 cggatgccag accttcgttg ttggcaaagt tcgccagtac aatctgcccc agcagttggg
    35461 tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc attgatttga taactcacca
    35521 gatcgcccgg tttgtagccg ttctgggtgg ttgccacaat gttgttagcg ccggtatttt
    35581 gctgcatgga gttgaggaag ctcaggctaa acgtggcggg atctgcgccg ttaattgcgc
    35641 cagtggaaat attcgctgtt ggatttgagg tcagaacgcc attggcatta aacaccagtg
    35701 ttgtcgctgt cttcgcaatg ctgtttggat cactactatc ctgggtgtag acctgccagt
    35761 tattatcccc ggtcttcaca aagtacacgc tcatatcatg agcattaccc tgactatcga
    35821 aaacagtcac cgaacctttt ttgttatagc tatccgcatt gctggcatca aatgcgttaa
    35881 cagagggaag cggatcactg gaattcaggt tgatctgcat cgacgccgtg gtggtagttt
    35941 tcgctgccat cagggtattc ggaatcgaaa tattggtcgg attcgcccct tgctgaatag
    36001 tcggcggcgt accggttgcc gggtaacccg tcagctgtaa accttgcata ttcaccaggt
    36061 tacggttttc atccagctta aattgtccgt tacggctgta gaacaccgaa ccattgctgt
    36121 ctaccagacg gaaaaaaccg ttctggctaa tagcaacgtc cagaccacgc ccggtgttgg
    36181 tggtcgtgcc atcggtaaag tcctgagtga taccagcaac ttttaccccc agtcccactt
    36241 tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg
    36301 agttggcgat attgttgcca ataacatcga ggttggtggc ggcagcgttt aatccgctaa
    36361 ccgcttgaga aaaggccatg actgactcct gaaatgtgaa ggcttaaatt atctgccgta
    36421 cttcgtcgag ggtagtggtg ccgtaagtgc cgagatccag cgtattaccg ttgttgccgc
    36481 ggatcacccc ctgcaccaga gcaaactgca gcggttgggc aaccagttgt gtaccaccgt
    36541 tactggcgct aatcgctaca ttgtaagaac cattcgtcgc agtgctgccg tcagtcaacg
    36601 taccgtccca ggtgaaactg tggactccgg cagtcagttc accgatatca atggtacgga
    36661 cgaccgcgcc gtttttatcg gtgatggtgg ccgtcacttt gtctgctgcc tgttgcaact
    36721 caacaccaaa cggcgtggtc gtggtcacag ccccttcttc actgccgatt cccgcaagaa
    36781 cagtggtacc ggggatcatc acgccgtgac caatcaggtt actggcctgt aacgactggc
    36841 tgttatcaat ctgtccggaa atagatccga gcgtggtatt gagtttttca atcccactga
    36901 ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt
    36961 cctggttttt cagctgcgcc accaacaaag tcagaaaact gctttgtaaa tctgcggcgt
    37021 tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc ggtattcgtc ggatcggtgg
    37081 tggttaccgc aatagacata gctttctcct ttattgaccg agcgtaaggg ttttcagcat
    37141 catgcttttc accgtgttga gcacttcaac attggcctga taactgcgtg acgccgacat
    37201 ggtgttaacc atctctccga cgacatcaac gtttggcatt ttcacgtagc cctttgcatc
    37261 cgccagcgga ttacccggtt cataaaccag tttgtccgga gcctgacttt ctataacatc
    37321 ggcaaccttt acgccgcctg tcgcagcacc tggcgcagcg ttaacctgga ataccacctg
    37381 ttttgcccga tatggctgtc catcgggacc ggtcacgcta tcagcattcg ccagattact
    37441 ggccgccacg ttcaggcgct gggactgggc agttaacgcc gacccggcga tatcaaaaat
    37501 attcagcagt gccatccgtt aatttccgct ctgtaaaacg ttcatcatgc ctttgatctg
    37561 cccgctcaac gcgctaaggc tcatctggta ttgcaggctg ttatcggcaa actgggtgcg
    37621 ttcgcgatcc atatcgacag tattcccgtc aagcgaaggc tggtccggaa tacggtattg
    37681 cagttctgcg gaaggaggcg tcagcgcctg cgccggaatg tgtttcgttg aggtcatcgt
    37741 cagagcaacc acactggttg catcccgtcc acgttgcatg acttttttaa gttcactggc
    37801 aaaatcgata tcgcgcgcct gataaccagg ggtatcggca ttggcgatgt ttgctgccag
    37861 cacttcctga cgctgggcgc gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc
    37921 gagcttatcg agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct
    37981 ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg
    38041 tcattgcgga caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgacaat
    38101 aaaacgtagc gtggcgatca tcgcgatact gttcagtccg ttaagtacgg cgagcaatct
    38161 cacatcgcaa ttgcacacct tttttagcgc ccaactcgcg ggggtaagtg atgaggttcg
    38221 tgtttctatt cgtacagcac ccaatctgct accgccgtgc gatcagccat tgctttcgat
    38281 gagcaataat tcccgtctgt ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa
    38341 acgatattta caggttaatg tgcaggccac aggaaattat gtggttgccg cgatgcccat
    38401 tgtgcgtgga ggaaagctgg aagctggcaa tgtcaaactg aaacgcggac ggctggatac
    38461 cttgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga
    38521 tctatcaccc gatcaaccta tccagttaac ccagtttcgc caggcatggc ggataaaagc
    38581 ggggcaacgc gtcaacgtta tcgccagcgg tgatgggttt agcgccaacg cagaaggtca
    38641 ggcgctgaac aatgcagccg tcgcacaaaa tgcgcgggtg cgcatggtat cgggacaagt
    38701 ggtcagcggc gttgttgatg cagatgggaa tattcttata aacctgtaag ctgttaaaga
    38761 ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca atgaggaata
    38821 aaccatgagt attgatcgca cttcgcctct gaagcctgta agcaccgttc aaccgcgcga
    38881 aaccactgac gcgccggtaa caaacacccg ggcggcaaaa acaacagcct ccaccagcac
    38941 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
    39001 tcttgaacgt gtcgaagcgt taaagctggc gattcgtaac ggtgaactaa aaatggacac
    39061 cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa gacttgcaga gtaattgatc
    39121 gtatgacgcg tcttgcagag atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa
    39181 cggtaatgga tcaagagcag caacatctct ctatggggca gatcaacggc agccagttgc
    39241 aatggattac agaacaaaaa agctctctgc tggcgacgct ggattacctc gaacagttac
    39301 gcaggaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
    39361 ctggcaaaac gcagcaacta cgccaactga atcaacataa cggctggtta ctggaaggac
    39421 agattgaacg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctgt
    39481 atggggcgaa cggtcagacc tcaacaaccc atcgcggcag taaaaagatt tcgatctgaa
    39541 gattaactcc ggcctgcact gcaggccgga atgtatcgtt acaccgtccg gcgggcaaat
    39601 tctttaactt tgaagcccag tactgccagt gcagcgaagt acgcggcaat ccccgccagc
    39661 acgaccgcca ttaaacgcag taaacgccag ggcatagtac ccaatgacca ctccggcatg
    39721 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
    39781 agaaacgcca tccagccggg ttgcggggta aagattttct gcttacgcaa ctgccagtaa
    39841 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc
    39901 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
    39961 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
    40021 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
    40081 aaggcggtaa atttaccgta ctggaacagc gaaacggtta acggaccgga aagaatgccc
    40141 aacgcaaccg cacttggcag cgccaacagg aaacaaagac gcagccccca gtccatcaaa
    40201 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgacgg tagcaaaatg
    40261 gtgccaagcg ccacgcccag cacaccggac ggaaactcca ttaagcggtc ggcgtaatac
    40321 atccacgaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
    40381 atctggctca cagagacgcc aaggattgcc ggtcccatct gtttcaccac acgcattgct
    40441 ccggcatcgt ggaagttaat gcgtggcaaa accagcatgc cgatcttctt cagatgaggt
    40501 agctgataca ccagctgcag gatgccgccg accgtaacag cccaggccag cgccagcacc
    40561 ggtggattaa agtacggcgc ggcaaacaac gcaaaaccaa tcatgctgat attgagcaat
    40621 gtcggggcaa acgccggaat tgagaagcgg ttccaggtat tcagaatcgc tcccaccagc
    40681 gacgccaggg agatcagcaa gatataggga aaggttatct ttagtagctg gctggtcagg
    40741 gcaaatttgt cggcagtgtc agcgaagcca ggcgcggtca ccatgatcac ccacggtgca
    40801 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
    40861 gagacaaaga cccgcgtggc gtcttcaccc tgcttgcttt tatattctgc cagaatcggt
    40921 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
    40981 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
    41041 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
    41101 tttaataaat tcatcggtgt tctaatccag acccacgggc aaaacgcctg caaggcaggc
    41161 gctgaaagag gcgaatagtc tacctggatt atggtgaatt gctaccgcca gatgttacag
    41221 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg ccagcacggc
    41281 ctgttcgccg gcggtttgcg gaactgtctg attttgcacg cattcaatga agtgccgcgc
    41341 acagccgaca aatccacgtt gctcgagcgt actctgccaa ccaggaatcg gtttatgcac
    41401 tacgccctgc ccgcgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc
    41461 atcagtcacg gcctgcacgg tttcacgctg acttccggcc cggcgatgca tacaggtggt
    41521 gatctgcaaa ggaccagcca aaaaatggtg ctcggcaaac agcatttcgc cggactcatt
    41581 ggtcagcaac gtaccgcttt cgagagttgc attgccgcca gacaaccaca gcgcggtatc
    41641 caccacatgc agataatcat ccagcaatgt gaaataaaga tcgtgtggcc cgacgctatt
    41701 ggtacgatgt ttatccattc ttagcgagga cgcggtggcg agttgcgttt ttaactcacc
    41761 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc
    41821 cagttccacc agccgttcag catcgcgcag attttctgcc agcggtttat cgacacagac
    41881 atgtaccccc gcattgagta acgtactgac cacgtcaaag tggctggcgg tgctggaatg
    41941 cacaaaaacc gcatcgcaac tggcggcaag gctggataac gaatcagcat aaggaatgcg
    42001 ccagctgtca caaattggca aggctttggc gcgcgtaggc gaccacgcac cttgtaacgt
    42061 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcgcgatgc cgcctaaccc
    42121 cactacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
    42181 gacgctgttt cagttctgcc acttcgattt ccagggcttc gacacgggcc tgtaaatcac
    42241 cgtcaaccgc gttcaacata tccgttaccg ccggcggatt ctcaacctca ccactgaaaa
    42301 gatgcatgta gcggctttcg cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
    42361 catcttcgcg actcgccagt tgttccagcg tcgactccac ttccgccata tcgctgaatt
    42421 catacattcg cgcggcgcgg ctgcgcagtt cccccggcgt ctgggcacca cgcaataaca
    42481 acgtggtgat taacgccact tctgcggcgc tcagtttcag atcgccaaat tctgaattac
    42541 aaaaacgttg ctcatatttg gtgacccgat taccaaaacc gctcaccgtg cgtagataat
    42601 gacgtttgac cagattatcc agctgttcct gcacttcaga ttcactcaga ttcataaccg
    42661 gttcacggtt cgttttctga ttacaggccg tgactacacc atttacggag agcgggtatt
    42721 gctccggcgt ggtcacctgt ttttccagca agcaaccaat cacgcgagct tccatggcag
    42781 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
    42841 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
    42901 tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc gtttattgtg cggcatataa
    42961 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
    43021 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
    43081 gcatgaaaag agccgcgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
    43141 tccgggtcga ataagccaaa gtaaaaagct gaaccttgtt tatgaaattc gctaatcatc
    43201 cctagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
    43261 ggcttgagga aatggcgatt ctctgcgtaa taatccgcca gacgccaggc atcacgatca
    43321 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
    43381 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
    43441 tatacctgcg caaccagacc ctgtgaaaag tccagaatgc cattttctgc attattcaca
    43501 tatttgataa aaactctcta tcaaagcgac gttttgataa aaaaatattg tccccgaagt
    43561 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
    43621 ggggaaatgt cccgcgtgtc gcaggcgagg aacctgggta aatatttcct gctcatcgat
    43681 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
    43741 gatcaaatgg gctgggccgc cgtcatggtc ggtattgccc tcggtctacg ccagtttatt
    43801 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
    43861 attgtcaccg gtatgctgat gcgtgccgcc ggattcgcca caatgggtat cgcccacgaa
    43921 ccgtggctac tgtggttttc atgcctgctt tcgggactcg gcggcacgtt gtttgatcct
    43981 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac agcgtggtcg ttttttctcg
    44041 ctgttgatga tgcaggacag tgccggtgcg gtcattggcg cattgttggg gagctggctg
    44101 ttgcaatacg actttcgcct ggtctgcgcc acaggggcag ttttgtttgt gctgtgtgca
    44161 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgtac gcccgttcgc
    44221 gaaggcatga cccgcgtgat gcgtgacaag tgttttgtca cctatgtcct gacgctggcg
    44281 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
    44341 gcgggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
    44401 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttaatg
    44461 gctgggctgt tgataatgtc attaagcatg atgccagtag gcatggtcag cggcctgcaa
    44521 caacttttca ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
    44581 gaaaccttaa gtgcttcgct ggcagacgca agagctcgcg gcagctatat ggggtttagc
    44641 cgtctgggtc tggcgattgg cggcgctatt ggttatatcg gtggcggctg gctgtttgac
    44701 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggcat tattggcatc
    44761 ttcactttcc ttgcgctggg ttggcagttt agccagaaac gcgccgcgcg tcgtttgctt
    44821 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag agaactgaca
    44881 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
    44941 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
    45001 gacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
    45061 gacagccgct atatgccaga aagcatggac atcgttcact atgtcgataa actcgacggc
    45121 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat
    45181 ggctacgcca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
    45241 actcctgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
    45301 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt acgtgcgctg
    45361 gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac tttcggaaga tgatattcag
    45421 ctatttccgc tcctgcgtaa tctgacgcta gtagccggaa tcaactggcc aagccgcgtt
    45481 gctgattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
    45541 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
    45601 tctgctggcg cgtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
    45661 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
    45721 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
    45781 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
    45841 ccatattgtt ctgacaaacc tgaccagcca aattggtcgc gaagagccga ataaggttac
    45901 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagccaga aagcgaccat
    45961 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
    46021 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
    46081 gttgctcccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
    46141 cctgcgcgaa gatggcagcc agggcgaagc aatggcgaag aaactggcga aaggcattga
    46201 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tcacgagggc gcattcgcgc
    46261 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
    46321 cccttcagcc ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg
    46381 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
    46441 taccagcgaa atttatggac gggctatcgt aatgcccaat ctggctccgc ccgtgaccac
    46501 cgttgaggct gccgtggcgt atcgccagcg cattcttgac gccgtacctg ccgggcacga
    46561 tttcaccccg ctgatgacct gttatttaac agattcgctg gaccctaatg agctggagcg
    46621 cggatttaac gaaggcgtgt tcaccgctgc aaaactttat ccggcaaacg caaccactaa
    46681 ctccagccac ggcgttacgt cagttgacgc aatcatgccg gtacttgagc gcatggaaaa
    46741 aatcggtatg ccgctactgg tgcatggtga agtaacacat gcagatatcg acatttttga
    46801 tcgtgaagcg cgctttatag aaagcgtgat ggaaccgctg cgccagcgcc tgaccgcgct
    46861 gaaagtcgtt tttgagcata tcaccaccaa agacgctgcc gactatgtcc gtgacggaaa
    46921 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
    46981 ggttggtggc gtgcgtccgc acctgtattg tctacccatc ctcaaacgca atatccacca
    47041 acaggcattg cgtgaactgg tcgccagcgg ttttaatcgt gtattcctcg gaacggattc
    47101 tgcgccacat gcacgtcatc gcaaagagag cagctgcggc tgcgcgggct gtttcaacgc
    47161 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctt tgcagcactt
    47221 tgaagcattc tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt
    47281 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    47341 gctggtgcca ttcctcgccg gagaaacggt acgctggtcc gttaaacaat aaaaatcgta
    47401 gcttcctgtt gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg
    47461 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    47521 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    47581 acgtatcggt acgttatgcc gcagcaaata atttatcggt tattggcgcg acaaaagaag
    47641 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    47701 tcagcgaata atatgcagtg atttttgttt ttgccgggtc gccccggctt ttttatttta
    47761 cttgcgaatt gtgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    47821 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    47881 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    47941 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    48001 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    48061 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    48121 caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt
    48181 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttcca ggtaaacgag
    48241 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    48301 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    48361 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    48421 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccacca
    48481 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggt
    48541 gaaaagtatg tcccgctggt cctccgcgcg caaatgctgt gggatgaact ttcccgccac
    48601 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggtcc ggctgactcc
    48661 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc
    48721 gacgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggt
    48781 ttatttgaaa ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    48841 ctggcgaagg aagcgggctg tgcgcagctg ttcaactgcc cggtcaccgc aattcgtcat
    48901 gacgatgatg gcgtaactat tgaaacggct gacggtgagt atcaggcgaa aaaagcgatt
    48961 gtctgcgcgg gaacatgggt aaaagaccta ctcccggagc tgcctgtcca gcccgtacgc
    49021 aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggct
    49081 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    49141 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    49201 tttgcggaag tggccagtga tgggtcagaa gccttcccgt tcttgcgcaa tgtattgccg
    49261 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    49321 tttattatcg atactctacc cgcccacgat aatacactgc tcattaccgg cctgagtggg
    49381 cacggtttta aatttgcgtc agttttaggg gaaatggctg ccgattttgc gcaagacaaa
    49441 aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg
    49501 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggttggtg aactatctca
    49561 ttaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta
    49621 tggatctgaa ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt
    49681 taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt ctacagcact
    49741 caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca ttatt
//
LOCUS       DAZFRE010000027        45984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFRE010000027 DAZFRE010000000
VERSION     DAZFRE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.027"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            309..1232
                     /gene="yicL"
                     /locus_tag="ACHKUI_001849"
     CDS             309..1232
                     /gene="yicL"
                     /locus_tag="ACHKUI_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6598050.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRQARHE"
     gene            complement(1236..2054)
                     /gene="nlpA"
                     /locus_tag="ACHKUI_001850"
     CDS             complement(1236..2054)
                     /gene="nlpA"
                     /locus_tag="ACHKUI_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6598051.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2276..2569
                     /gene="yicS"
                     /locus_tag="ACHKUI_001851"
     CDS             2276..2569
                     /gene="yicS"
                     /locus_tag="ACHKUI_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6598052.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2610..3800)
                     /gene="nepI"
                     /locus_tag="ACHKUI_001852"
     CDS             complement(2610..3800)
                     /gene="nepI"
                     /locus_tag="ACHKUI_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6598053.1"
                     /translation="MSEFIAENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4011..4463)
                     /gene="yicN"
                     /locus_tag="ACHKUI_001853"
     CDS             complement(4011..4463)
                     /gene="yicN"
                     /locus_tag="ACHKUI_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6598054.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4516..5851)
                     /gene="adeQ"
                     /locus_tag="ACHKUI_001854"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             complement(4516..5851)
                     /gene="adeQ"
                     /locus_tag="ACHKUI_001854"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            6026..7792
                     /gene="adeD"
                     /locus_tag="ACHKUI_001855"
     CDS             6026..7792
                     /gene="adeD"
                     /locus_tag="ACHKUI_001855"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6598055.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LSPGKQADIVLLSDVRKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDTLKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEFTE"
     gene            complement(7839..9230)
                     /gene="uhpT"
                     /locus_tag="ACHKUI_001856"
     CDS             complement(7839..9230)
                     /gene="uhpT"
                     /locus_tag="ACHKUI_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6598056.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9368..10687)
                     /gene="uhpC"
                     /locus_tag="ACHKUI_001857"
     CDS             complement(9368..10687)
                     /gene="uhpC"
                     /locus_tag="ACHKUI_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6598057.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10697..12199)
                     /gene="uhpB"
                     /locus_tag="ACHKUI_001858"
     CDS             complement(10697..12199)
                     /gene="uhpB"
                     /locus_tag="ACHKUI_001858"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6598058.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(12199..12789)
                     /gene="uhpA"
                     /locus_tag="ACHKUI_001859"
     CDS             complement(12199..12789)
                     /gene="uhpA"
                     /locus_tag="ACHKUI_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6598059.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12862..13152)
                     /gene="ilvN"
                     /locus_tag="ACHKUI_001860"
     CDS             complement(12862..13152)
                     /gene="ilvN"
                     /locus_tag="ACHKUI_001860"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6598060.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13156..14844)
                     /gene="ilvB"
                     /locus_tag="ACHKUI_001861"
     CDS             complement(13156..14844)
                     /gene="ilvB"
                     /locus_tag="ACHKUI_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6598061.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(14950..15048)
                     /gene="ivbL"
                     /locus_tag="ACHKUI_001862"
     CDS             complement(14950..15048)
                     /gene="ivbL"
                     /locus_tag="ACHKUI_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6598062.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(15293..15367)
                     /gene="ysdE"
                     /locus_tag="ACHKUI_001863"
     CDS             complement(15293..15367)
                     /gene="ysdE"
                     /locus_tag="ACHKUI_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6598063.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            15449..16633
                     /gene="emrD"
                     /locus_tag="ACHKUI_001864"
     CDS             15449..16633
                     /gene="emrD"
                     /locus_tag="ACHKUI_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6598064.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(16641..17138)
                     /gene="yidF"
                     /locus_tag="ACHKUI_001865"
     CDS             complement(16641..17138)
                     /gene="yidF"
                     /locus_tag="ACHKUI_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6598065.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17135..17497)
                     /gene="yidG"
                     /locus_tag="ACHKUI_001866"
     CDS             complement(17135..17497)
                     /gene="yidG"
                     /locus_tag="ACHKUI_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6598066.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(17487..17834)
                     /gene="yidH"
                     /locus_tag="ACHKUI_001867"
     CDS             complement(17487..17834)
                     /gene="yidH"
                     /locus_tag="ACHKUI_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6598067.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(17894..19387)
                     /gene="yidJ"
                     /locus_tag="ACHKUI_001868"
     CDS             complement(17894..19387)
                     /gene="yidJ"
                     /locus_tag="ACHKUI_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6598068.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVGFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(19384..21099)
                     /gene="yidK"
                     /locus_tag="ACHKUI_001869"
     CDS             complement(19384..21099)
                     /gene="yidK"
                     /locus_tag="ACHKUI_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6598069.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            21266..22132
                     /gene="yidL"
                     /locus_tag="ACHKUI_001870"
     CDS             21266..22132
                     /gene="yidL"
                     /locus_tag="ACHKUI_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6598070.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYAVGKKDPQRRQIEKLIATLHANLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(22222..23883)
                     /gene="yidE"
                     /locus_tag="ACHKUI_001871"
     CDS             complement(22222..23883)
                     /gene="yidE"
                     /locus_tag="ACHKUI_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6598071.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(24080..24508)
                     /gene="ibpB"
                     /locus_tag="ACHKUI_001872"
     CDS             complement(24080..24508)
                     /gene="ibpB"
                     /locus_tag="ACHKUI_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6598072.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(24620..25033)
                     /gene="ibpA"
                     /locus_tag="ACHKUI_001873"
     CDS             complement(24620..25033)
                     /gene="ibpA"
                     /locus_tag="ACHKUI_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6598073.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(25027..25099)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            25339..25671
                     /gene="yidQ"
                     /locus_tag="ACHKUI_001874"
     CDS             25339..25671
                     /gene="yidQ"
                     /locus_tag="ACHKUI_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6598074.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQETNNVI
                     PPAKMPAN"
     gene            complement(25675..26889)
                     /gene="yidR"
                     /locus_tag="ACHKUI_001875"
     CDS             complement(25675..26889)
                     /gene="yidR"
                     /locus_tag="ACHKUI_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6598075.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHTKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRIYPGLVNIPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            26990..28088
                     /gene="cbrA"
                     /locus_tag="ACHKUI_001876"
                     /pseudo
     CDS             26990..28088
                     /gene="cbrA"
                     /locus_tag="ACHKUI_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
     gene            complement(28049..29386)
                     /gene="dgoT"
                     /locus_tag="ACHKUI_001877"
     CDS             complement(28049..29386)
                     /gene="dgoT"
                     /locus_tag="ACHKUI_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6598076.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(29461..30609)
                     /gene="dgoD"
                     /locus_tag="ACHKUI_001878"
     CDS             complement(29461..30609)
                     /gene="dgoD"
                     /locus_tag="ACHKUI_001878"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO6598077.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(30606..31223)
                     /gene="dgoA"
                     /locus_tag="ACHKUI_001879"
     CDS             complement(30606..31223)
                     /gene="dgoA"
                     /locus_tag="ACHKUI_001879"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6598078.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(31207..32085)
                     /gene="dgoK"
                     /locus_tag="ACHKUI_001880"
     CDS             complement(31207..32085)
                     /gene="dgoK"
                     /locus_tag="ACHKUI_001880"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6598079.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTGWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLEHGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVDHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(32082..32771)
                     /gene="dgoR"
                     /locus_tag="ACHKUI_001881"
     CDS             complement(32082..32771)
                     /gene="dgoR"
                     /locus_tag="ACHKUI_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO6598080.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            33050..33706
                     /locus_tag="ACHKUI_001882"
     CDS             33050..33706
                     /locus_tag="ACHKUI_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598081.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(33750..34562)
                     /gene="yidA"
                     /locus_tag="ACHKUI_001883"
     CDS             complement(33750..34562)
                     /gene="yidA"
                     /locus_tag="ACHKUI_001883"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6598082.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(34677..35075)
                     /gene="yidB"
                     /locus_tag="ACHKUI_001884"
     CDS             complement(34677..35075)
                     /gene="yidB"
                     /locus_tag="ACHKUI_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6598083.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            35193..35294
                     /locus_tag="ACHKUI_001885"
                     /pseudo
     CDS             35193..35294
                     /locus_tag="ACHKUI_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35315..37729)
                     /gene="gyrB"
                     /locus_tag="ACHKUI_001886"
     CDS             complement(35315..37729)
                     /gene="gyrB"
                     /locus_tag="ACHKUI_001886"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6598084.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(37758..38831)
                     /gene="recF"
                     /locus_tag="ACHKUI_001887"
     CDS             complement(37758..38831)
                     /gene="recF"
                     /locus_tag="ACHKUI_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6598085.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(38831..39931)
                     /gene="dnaN"
                     /locus_tag="ACHKUI_001888"
     CDS             complement(38831..39931)
                     /gene="dnaN"
                     /locus_tag="ACHKUI_001888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6598086.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(39936..41339)
                     /gene="dnaA"
                     /locus_tag="ACHKUI_001889"
     CDS             complement(39936..41339)
                     /gene="dnaA"
                     /locus_tag="ACHKUI_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6598087.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            41636..41716
                     /locus_tag="ACHKUI_001890"
     CDS             41636..41716
                     /locus_tag="ACHKUI_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598088.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            41946..42086
                     /gene="rpmH"
                     /locus_tag="ACHKUI_001891"
     CDS             41946..42086
                     /gene="rpmH"
                     /locus_tag="ACHKUI_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6598089.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            42103..42462
                     /gene="rnpA"
                     /locus_tag="ACHKUI_001892"
     CDS             42103..42462
                     /gene="rnpA"
                     /locus_tag="ACHKUI_001892"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6598090.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            42426..42683
                     /gene="yidD"
                     /locus_tag="ACHKUI_001893"
     CDS             42426..42683
                     /gene="yidD"
                     /locus_tag="ACHKUI_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6598091.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            42686..44332
                     /gene="yidC"
                     /locus_tag="ACHKUI_001894"
     CDS             42686..44332
                     /gene="yidC"
                     /locus_tag="ACHKUI_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6598092.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            44438..45802
                     /gene="mnmE"
                     /locus_tag="ACHKUI_001895"
     CDS             44438..45802
                     /gene="mnmE"
                     /locus_tag="ACHKUI_001895"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6598093.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNNVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11377 a        12421 c        11553 g        10633 t
ORIGIN      
        1 atcaacacct gaacgacagt tttctatttc tgttctgaat gatgacaaca aagaaaatat
       61 acctaccgat accgcacctt gttttacaat tggtattcct gatgaatata ctaaagccat
      121 agagttattg aacattaaat aattaaattt catagtgagt ttttttaaat accatatgaa
      181 taattgttat atgtcattat ttcctccatt cactaaaact gattgattac tctactccct
      241 caccgtcatc ggtagtggta tccttaacgt atattgatga aaagacaaaa cagtatgcag
      301 agggaaaaat gggttccacc agaaagggga tgctgaacgt tctgattgcc gccgtgttgt
      361 ggggaagttc aggggtctgc gcgcaataca tcatggagca aagccagatg tcgtcgcagt
      421 ttttgactat gacgcgtttg atattcgccg gtttgattct gctgacgcta tcatttgttc
      481 atggtgataa aatcttttct attattaaca atcacaaaga tgccattagc ctactgattt
      541 tttccgtggt tggcgcgcta accgtacagc tcactttttt gctaaccatc gaaaaatcga
      601 acgcagccac ggcaacggtg ctgcaattcc tctcaccgac tattatcgtc gcctggttct
      661 cactggtgcg taaatcccgc ccgggcattc tggttttctg cgctattttg acatcgctga
      721 tcgggacttt tttattggtg acacacggta atccgacgtc attatcgatc tctcctgccg
      781 cgttattctg ggggattgcc tcggcatttg ctgctgcatt ctataccacc tatccctcaa
      841 cgctgattgc ccgctatggc acgttaccgg tcgtcggctg gagtatgctg attggcggtc
      901 tgatcctgct gcccttttat gccagacaag ggacaaactt tgtcgttaac ggcagtttga
      961 ttctggcgtt tttttatttg gtggtcattg gtacgtccct gacatttagt ctgtacctga
     1021 aaggagcaca attaattggt ggtccaaaag ccagcatttt aagctgtgca gaaccattaa
     1081 gtagcgcgct gctctctttg ttgttgctgg ggatcacctt cacattgccg gactggctgg
     1141 gaacgctgct gattctgtca tcggtgattt tgatttcaat ggattcccgc cgccgcgcca
     1201 gaaaaataaa tcgtcaggcg cggcatgagt gagaattacc agccaggcac cgcgccaccg
     1261 ttaaagatgg tttctgccgc tttagcgact tcgggtgatt gataagattg cagaaattcc
     1321 ttcacgtttt ctgcattctt attatcttcc cgtgccacca gaatattcac atacggcgaa
     1381 tttttatctt caataaatac gctgtcgtta accggagaaa gtcctgtttg ctggatgtaa
     1441 gtggtgctga taatcgctac atccactttc gggtcgtcca gtacacgtgg caactgtgcc
     1501 ccttccagct ccattatctg caaatggcgt ggattatcgg ttatatccaa cgcggtaggt
     1561 aataaccctt ttccctcttt cagagtaatt agcttctctt tttgcagcag taaaagcgcg
     1621 cgaccaaggt tagtcgggtc gttggggatc gccactgtcg caccttcttt tatttgcgcc
     1681 actgttttga tttttttgga atagcccgcc atggggaaga caaacgtatt tcccaccgct
     1741 accagtttat aaccatgcgc ctgattatct tgttcaagaa agggtcgatg ctggaagacg
     1801 ttcgcatcaa gctcgccatg atttgtcgca tcgttcggta gcaacgaacc gctaaatcct
     1861 accagttcaa catcgaggcc atatttctct tttgctacct ttttagcgac ttccgcgaca
     1921 tcttgttctg cgccattaat aacgccaact ttaatatgct tttcatcgct gctgctctgg
     1981 tcgcaacctg ccagcagaac tcctgccagt aataatgcgg cccccgcccg tagatgatgt
     2041 gttgtcagtt tcattttttt atccttttat taatgattac ctgactatag cggtaaggtc
     2101 gctgcagttt aaaaaacgaa acgctatcga taagaataaa aaggaataaa agtggaatat
     2161 aaagtctggc acttcttact gaccacgcaa acacctttcg tacagcatga tgagagcggc
     2221 aaaagcaaac tacacttatg cttcattcga tgcacatttg cagaaggttg ccgctatgaa
     2281 gccaacgatg ctactcatga ttaccgtatt cctgattttt ccagccattt ctcaggccga
     2341 gtcgccattc agttctctac agtcggcaaa agagaaaacc acggtattgc aagatttacg
     2401 taaaatttgc acgccacagg cgtcattatc agatgaagcg tgggaaaagt taatgttgtc
     2461 agatgaaaac aataaacaac atatacgcga ggccatcgtg gcgatggaac gtaataatca
     2521 gagcaattat tgggaagcgt tgggtaaggt agaatgcccg gatatgtaag aacggcaaca
     2581 accactgacc aacgcttctt tacctgatat caggatttct tcattttcac ctttgcagtc
     2641 accaacaatg cagtcagcaa catcaatgtg ccggacaaca tcagcggcga agtcagacca
     2701 atgttatcca acgcataacc gccgattgcc gcaccgcagg tgttagcaag ctgaataacg
     2761 gccacctgaa tagatccagc cttttctgcc tgatcggcca atgagcgagt gatccacgtt
     2821 gaccaaccaa cgggaattaa agcaaaggtc agcccccaga taatcgccac accagtagca
     2881 acaattttat cgcttcccca cagcgtcagt accagcgcgc tcagggcgag cacaaacggt
     2941 gcgcccgcta aggccagttt caccgaacgt ttgagaataa acgacgaaag cgatgtcccg
     3001 acaaagctgg cgataccaaa actcagcagc accagggtta agccatcaac accgaatccc
     3061 gccagattca tatacactgg gcgaatataa gtgaaaaaag caaactgtcc ggcaaaggac
     3121 ataaagatgg cgatcatccc ggccatcact cccggacgtt gtaataagcg gaaagtattt
     3181 tgtttctgat gcgaaggttc acctggcagc gaaggcagtg atttgataat ccagaaaata
     3241 cacagcacac ccatcgccgc cgccgcatta aagacattgc gccaaccgat aagctcgcct
     3301 aaaaaactgc ccaacggcgc ggcaatcacc agcgcaatag aaaccgcgcc gaagatcacc
     3361 gacagcgcct tcggcaccgt acgcggcggt accagacgca tggtcagcga cgccgacatc
     3421 gcccagaacc cgcccagcgc cagccccaga caggcgcgac cgattaacag cagactgaat
     3481 gagttggcaa aggaaaccag caaacaggaa agcgtcagca aaacggcgaa cagaatgaca
     3541 acgtagcggc ggtcagtggc ctgaatggtc tgggtaataa acaaactggc aaacatcgcc
     3601 acaaaggcgg tcacggtcac cgattgcccg gcaacccctt ccgaaatgcc taaatcctgg
     3661 gccattggcg tcaacaaact gacaggcaaa aactcaacga taatcagaca ggcgacgcaa
     3721 aacgccaccg agaaaacggc tgaccagttc ggtcgggtga tggcattcgc gccgcggttt
     3781 tcagcaataa attcactcat ggtgttaccc gtggcatgtt tcaggggaaa agccgaccag
     3841 tctaacattg aaatgtgtga tgcatttaac gtttttgcaa cttttacgat gttgcttgag
     3901 ctggagttaa gatcgaaacg gaacagggca aagattaccg gatgcggtgt gaacgtctta
     3961 tccacccacc aggtcaggcg cggtctgtag gctgataaga cgaagcggtt ttaatgcagc
     4021 cagtgaacgc cctcggcagg ctgaaagaac gcgttatagc gcatctgaaa agcattgata
     4081 tcctgcattt caggttcggt ttcgcgcaga aaacccagcg ccagcctgac ctgagcatca
     4141 gtaatcggcg cggcaaggcg ggctttttgt aaaatccgat gccattgcag caggttctgt
     4201 tcgctaccat cgacaatgac aatttgccag atgagcaaca cctgcgcggc gttttgcagg
     4261 tgcgactcat ccgggcgatg caaataacgt aaaaattcgt caccggctga ctttcccatt
     4321 tgatcgacca tcgactccac gggtacgaca tcgatgttca gccgatcgct gagacggcga
     4381 atcgcttttc tggccccgga tgtcagcacc cgaaaaccaa ccacaaacac taccgccagc
     4441 gtggcgagca ttatccagat catgagttct cctgcgtaat cagaactcat gatatctgga
     4501 aaccctcgcc agttactaat ccaccagaat aatcttcagt gcaaacagag ttgcgaccac
     4561 caccacacac aggttcagat cgcgccagcg cccggtgcac actttcatga tgcagtacga
     4621 cataaagcct agtgcaatcc cttcggtgat cgagaaagta aagggcatca tcaccgtggt
     4681 aataaacgca ggcaccgatt cggtaaaatc atcccagtta acgcgcgcca ggcttgaagt
     4741 catcagcacg ccaacaaaga ttaacgctcc ggcggttgcg taaggaggaa ccatcgccac
     4801 cagcggtgag aagaacataa ctaacaggaa cataacgccg actacgacag ccgtcagacc
     4861 cgtgcgacca ccgacagcca cacctgaagt actttcaata taggcggtaa cagacgaggt
     4921 accgataaac gcacccgcca ccgaactgac gctatcaaca cacagcgcct tattcatatt
     4981 ggggaattta ccgttactat ctattaagcc agctttatca gtcacaccaa ttaatgtccc
     5041 tgatgaatca aataagttga tcagcataaa ggagaaaatg ataccggcga gttcaagtga
     5101 taacgcaccg ctcaaatcca cttcaccaat gacgccgcta atatcaggcg gaatggaata
     5161 gacgccgcta aaatgaacat ctccgaaaaa caatccacag caagacgtca ccacaataga
     5221 aacaagcacc gcggcatgaa aatgacgtga tgacaacacg gttataataa aaaaccctaa
     5281 aatacctaac aacacgccgt gagaacttaa atcgccaatc atcaccaggg tgtctttatt
     5341 ggcgacaata acgccagtat ttttttaatc ccattaaggc gataaataat ccgattccgc
     5401 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     5461 acagcgtgag caaaaatagc ccaactgcgc cccagaatat cgcgcccatc ccggtctgcc
     5521 aggagatacc catcgccccc acgaccacga aggcaaagaa ggcgttcagc cccattgccg
     5581 gagccagtgc cacgggtaag ttagcaaata cccccatcgc aatactgccg ataccggcaa
     5641 tcaaacaggt ggtaacaaac accacttttg ggtccatttg tgccgcgccg aggatttgcg
     5701 ggttcacaaa aacgatgtac accatggtta aaaatgtggt catccccgca atcaattctg
     5761 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     5821 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     5881 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     5941 acattaatgg atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     6001 ttgataaaat caaaatgaga aaaatatgaa taattctatt aaccataaat ttcatcacat
     6061 tagccgggct gaataccagg aattgttagc cgtttcccgt ggagacgctg ttgccgatta
     6121 tattattgat aatgtctcta ttctcgacct gatcaatggc ggagaaattt ccggcccaat
     6181 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgatg ctccggcttt
     6241 gcagcggatt gatgcccgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     6301 tattgaatcc agcatgatga cgccggtcac ctttgaaacc gctaccctgc cgcgcggcct
     6361 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggcgaag ccggattcgc
     6421 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     6481 cgtacccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     6541 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     6601 aattagcggg cagaatgcgc tgctcgataa actggacgca tttcgccacc tgacgctgga
     6661 cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc tatattgctg cgggtattga
     6721 aaactgccac gaaagttatc agttggaaga aggacgccgg aaattgcaac tcggcatgtc
     6781 attgatgatc cgtgaagggt ccgcagcccg caatctcaac gcgctggcac cgttgatcaa
     6841 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     6901 ccatgaagga catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     6961 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cattttggtc tgaatcacct
     7021 cggcttactg tcacccggca agcaggccga tatcgtcctg ttgagcgatg tgcgtaaggt
     7081 cacggtgcag caggtactgg tgaaaggcga gccgatcgat gcacaaacct tacaggcgga
     7141 agagtcggcg agactggcac aatccgcccc gccatatggc aataccattg atcgccagcc
     7201 agtttccgcc agcgactttg ccctgcaatt tacccccgga aaacgctatc gcgttattga
     7261 ggtaatccat aacgaactga tcacgcactc ccgctccagc gtctacagcg aaaacggttt
     7321 tgatcgcgat gatgtgtgct ttattgccgt tcttgagcgt tacggacaac gactggctcc
     7381 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggt gcgctggcgg cgacggtcag
     7441 ccatgacagc cataatattg tggtgatcgg tcgtagcgca gaagagatgg cgctggcggt
     7501 caatcaggtg attcaggatg gcggcgggct gtgcgtggtc cgtaacggcc aggtacaaag
     7561 tcatctaccg ttgcccattg ccgggctaat gagcaccgac acggcgcagt cactggcgga
     7621 gcaaattgac accttgaaag ccgccgcccg tgaatgcggt ccgttaccgg atgagccgtt
     7681 tattcagatg gcgtttcttt ccctgccagt gatccccgcg ctgaaactaa ccagtcaggg
     7741 gctgtttgat ggcgagaagt ttgccttcac tacgctggaa ttcacggaat aataaaaaaa
     7801 agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga
     7861 atttttttct cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag
     7921 atacaaccaa tagcggcgat atccagcgcg gcgaaggtgc ctgcccagcc ggtaaggcca
     7981 aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc
     8041 aggtaagcga aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag
     8101 ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag
     8161 agagaagcca ggtagatata ttggttgctg gcgtgttgat acacaccgag cgtggcgata
     8221 atcagcgcca gcgcgataca ggccaccagg ccacggcgac cgttcgccag gtcagagagc
     8281 cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc
     8341 gccaccgctt tagagagttt cagttcctgg aacgcataca cggttgacca ctggtcgata
     8401 ccaatacgca ccacatagag gaaaatgttg gcaaagcaca gcagccagat cactttgttt
     8461 ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctcttta
     8521 tcctcttcac tgatctcctc gccaaacagc tcttcagctt tgccgaggcc ataagattcc
     8581 ggggagtcgc tgccgtaacg cagaccaata aaaccaacaa tcagcgcgat aatcgacggg
     8641 aaaataaaca tgccgatgac atgaccatcg aacaggtaat ttgcgccgaa cagcgccaca
     8701 cctgctgcgc ctgcaccacc aaggttgtga gaaatgttcc agaaaccgag gaatgtcccg
     8761 cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc gccggtactc
     8821 tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg
     8881 cccatactgg cgctgaagcc cagcatacaa atggcagaca ggatcagcat gaacggcagg
     8941 aattgtttgg tgtttttgcc gtcggcgtag taagaaacca gcgttttacc cacgccataa
     9001 gtgatggaga agcccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgag
     9061 atcatgtcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt aaggtagccg
     9121 ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg
     9181 agcggaaggt ccagggtcgg cttgcgaacc tggtttaaga aagccagcat gggttactcc
     9241 tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga
     9301 tagcgtccag gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg
     9361 agatgcatca cgcttctcgc ggtgtctggg cgttcaaaaa gggcagtaat agcagtgcgg
     9421 aaatcccggc ggcgatggcg ataaccacaa aaaatccgct ccagtgccag gtatcgagta
     9481 ctttcgccag cggccaacca gcaagcgacg caccaagata agcaaacaag ccgacaaacc
     9541 ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccatg ccgattaaca
     9601 tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc atcacgtagc
     9661 tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg gcgaaaatca
     9721 aattcatcgg cccgcggttg ccgttaaaca gtttgtccga accccaaccc gctaccagcg
     9781 caccgataaa tccgcccagt tcaaacattg tcactgccgt attcgccgtg accagatcga
     9841 cgcccagcgt ctcggacata tacaaattgc cccagtcgtt tatcgccgcc cggaccacat
     9901 agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac acatatttgg
     9961 tgaggatctc tttacgcgtc agccctgccc cttcttgttg ttgagcaatc tccagtgcgt
    10021 cgtgccgcca ttcaccaacc gccggtaaac ctaacgcctg cgggcgatcg cgcagccgcc
    10081 agcagagaaa aatccccacg actatcgcca tacaacccgc aatcatcatc ccggcacgcc
    10141 agccgtaatg cagcgcgctc gctgccatca caatgggaat gagtgcgccg ccgacgttat
    10201 gtgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgagaatac caggcggtta
    10261 acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcaac acccaaagca
    10321 cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttgataatg cccgtggcaa
    10381 taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg ccggagacaa
    10441 acttcgacac gccgtaggta atgtaaaaca gggtcgctaa caggccaata tcgctacggc
    10501 tgagcacgcc gttagcaagg atttctggta cggcggcgtt aaaactttta cgcgtgaagt
    10561 aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgatagc
    10621 gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg
    10681 gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca cgcgtgccgt
    10741 gcagacagga aatggtcaat gtgccaccca gcgccgttac gcgctcgcgc attccggtaa
    10801 ggccaaaacc gtgttgcccg gaatctggcg gtaaaccgct gccatcgtct tcaatcacca
    10861 gcattaaccg ctcatcatgc tgccagcctt gcagcgtgac cgcgctggca tcggcatgtt
    10921 tcacaatgtt gttcaaccct tcctggcaga cgcgaaacag cgtcacgcgc tggttttcgc
    10981 ttaacgctga ttcatcgatt cgccattcga gatggctgac aatgccgcgc ccttccagct
    11041 ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc agctggcgcg
    11101 gacgtaaccg ccctaacaag cggcgcaccg cgtcgtaaac gcccaacgac aactgttcga
    11161 tgagctgccc gctctgtttc acgctggcgt tatccgccgc gagccgctgg acaatgcccg
    11221 cttgagttcg aatagcagta atggtctgac cgatatcatc gtgcagctca cgtgccacat
    11281 cacggcgcac gctctcttcg gtttccagca accgttcagc gagatgctga ttgcgcgcca
    11341 gttccttttg cagcgactgg ttaagttcac gcaaccgctg gatgccagcg cccagtaaca
    11401 accctgtcag actttgcacc agcagcgaga gcaataaatc caccggatga tcgcgccagg
    11461 tttgactggc gatcagcgcg atggcgttca tcaatgtcgc aatcagcgct ccttgccaac
    11521 catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta aagcgcgaca
    11581 gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc aagtaccaga
    11641 ccagatgccg cccacgccag ttgattggtt gagaaaccag tgacgggccg agcggcagcc
    11701 aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc gtcaggccgc
    11761 cagttaaagt cagcagtagc gcattccacg actctttgcc gtgccaaagc cagggcagcg
    11821 actgcaacaa cgccgccgcc gttaatgccg caccctgtaa cagcaaggtg cgccagtcac
    11881 gttgatggcg atagcgcgag atcagcgcca cgggcagtaa cgtcagtaaa ctaccgatca
    11941 tcaataagga gaaatgggtt aaaccgaccg cctgcgttaa ccagtaaatc agcagccact
    12001 ccgcgcccag caacaccggc cagtatccgc gcgggcattg cagcattagc cctagccgca
    12061 ggccaaacgg aaataacagt accgccatat cagggcgctc aaccaggtgc aggctgatac
    12121 tccacaggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc
    12181 gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagtt caacgtcgtt
    12241 actgacgccc agtttttcca tcaggttggc gcgatggaca tgtaccgttt tcggtgacaa
    12301 gcccagttcg gcagcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg
    12361 gcgttcgcgt ttggttagcg ggtcctggcg accggatgcc agtttaatgg caatatccgg
    12421 cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt catccgggct
    12481 acagcgtttg gagagaaagc cgcgcgcccc cgcgttaagc gcctgctcaa ccagcgccgg
    12541 actgtcatga acggagagca taattgtcgc catgcctttc ggcagctggc ttagaagctc
    12601 cagaccggag atatcaggca tggagatatc gcaaatacac acctgcacac cgcgccccgg
    12661 cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca aatcaggttc
    12721 cagccccagc agctgcgcaa agccggagcg gacgataagg tgatcgtcta taagggcaac
    12781 ggtgatcatg atcttgtcct ggcgggtaat aaaacgcgct taccttaacg ataagcgcga
    12841 tgttgttcaa gccttgagcg gttactgaaa aaacaccgca atcttgttaa acatggtcgg
    12901 atcggactga ttacgctgca ctttcaccac atcttccagc ttgtcgattt ggcttatcat
    12961 ctgctccaga cgctggtcgt cattgaccag tagccagatg tggcttttgt cgctgtcctg
    13021 aatcggcagg cagagaatgc cttcaacgtt aaaagcgcga cgggcaaaaa ggccacaaac
    13081 gtgggtcatt acacccggat ggttgcgaac ggtgagttcc agaatcacgt tatcatgagt
    13141 tgtgttttgc atggcttatt cccccaccat ttcagtattc gccgcacctg gcggcaccat
    13201 cgggtacact ttttcttcgg catcaatgcg cacatggatc agcgccgggc cagggcgatt
    13261 gatgatttcc tgcaatgcag cctgcggatc ggcttcgtta ttcaaatcac aggtttcgag
    13321 gccgaatccg gcggcaatct gcataaagtt gattttgccc ggataggtag cggcaaaaac
    13381 gccctgcttg tagaacagac tctgttgctg atgcaccagc cccagcgctt cgttgttcat
    13441 caaaatgatt ttaacatcca gctgattttc actggcggtc gccatctcct gaatattcat
    13501 catcaggctg ccgtcgccgg agaagcacaa cactttgcgg tccgggttcg ccagcgccgc
    13561 gccaatcgcc gcaggcaggc caaaacccat cgtgcccagc ccgccggagg tcagccactg
    13621 gcgtgggcga ttgagcggat aggcttgcgc ggtccacatc tgatgctgac ccacgtcggt
    13681 ggtgataatc gcattgtcat cgacacaggc ggcaacggcg ttgatcaggc cgtaatggct
    13741 taatggatcg cacgctttcg ggattggaca cgggaattca cgctgcaaat ccgccaccaa
    13801 ctggtgccac tctgcacgcg gttgcgcttc caccagcggg atcaactgcg ccaacacgtc
    13861 atcaacatcc gcctgaattg ccacatgcgg ttgcttgatt ttgcccagct ctgcgcggtc
    13921 gatatcgaca tgaataattt tggcattcgg acagaactgc tcggttttgc caatcgcccg
    13981 gtcatcaaaa cgcgcaccga gaacaatcaa caaatccgcc tcctgcaaaa tatagttggt
    14041 gctgcgcacg ccgtgcatcc ccagcatacc cagcgacagc ggatgcgctt ttggcaacat
    14101 acccagcgcc attaaagtca tggtggtagg cagttgcgct ttctccgcca gttcacgcac
    14161 tcgcgcaggc gcgttgatca caccaccacc cagataaagc accgggcgtt tggcggcgtt
    14221 aatcatcgcc gccgcgtcac gaatactttc ttcgctaaag gcgggtgcgg cggctttttc
    14281 tgccatagcg ggctgtgttt caatctcaaa aactgccgtt tgcacatcct taggaatgtc
    14341 tatccacacc gggccagggc ggcctgattg cgcaatacgg aaggcatcgc tcatgacctg
    14401 cgggagttct tcgatatgtc tgaccagata gttgtgtttg gtgatgggga tagagatgcc
    14461 gtaggtgtcg acttcctgga aggcgtcggt gccgatcatc gaggcgggaa cctgaccagt
    14521 gatgcaaatc agcgggatgg agtccagcct cgcatcggca atggcggtca ccaggttagt
    14581 cgcacccggt ccactacagg ccatacagac cgccggttta ccgtcggtgc gcgccattcc
    14641 ctgagcgata aaccccgcgc cctgttcatg gcgagccaga atatggcgga tttgcgtgct
    14701 ttggcttaag gcatcgtaaa caggcaggat agaaccgccc ggaatacccg tcacaatctt
    14761 aatgccctgc tgttccagga aatgaacgat aaattctgcg ccggtaaagc gcttacgcgt
    14821 cgatgttgtg cccgaacttg ccatgctcca gtccttttct tctgggccga ctttccggga
    14881 ggagcttaaa cgaaaaaccc cgcccggttt gcgccggcgg ggttttggaa tcgtgtgttg
    14941 ttccagtccc tacggcgcat tgccgacgac caccaccaca cgcacgacga ccactgcggc
    15001 ggatggcgca gttggtagta gttttgcgtt gaacatggaa gtagtcattg gggaccttgt
    15061 tggttttgtg tttaacaata tttatacaag cacagcttta caggggagac aatggaaaat
    15121 ttttcttccg gataaaaata cgtggcagat cacattttga actgaaactc aggcaataaa
    15181 gcatcatcac ctgaaagacg gtcagattgt tgacagagtg cgccttattc atgtcgaata
    15241 cagcgtaaac accttaccct accaaccgca ccatcactgt ttccggcaac atttattgcc
    15301 gcttttgttt acatattctg ccgctaaaca attccccatt cctggcgtat atctggctaa
    15361 cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt gacttattct
    15421 aattattttt ataaaagcat ccgtgataat gaaaaggcat agaaacgtca atttgttatt
    15481 gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata ttccagctat
    15541 tgccgatatg gcgcgcgatc tcaatgtccg tgaaggggcg gtgcagagcg taatgggcgc
    15601 ttatctgctg acttacggtg tctcacagct gttttatggc ccgatttccg accgcgtggg
    15661 tcgccgaccg gtgatcctcg tcggaatgtc catttttatg ctggcaacgc tggtcgcggt
    15721 cacgacctcc agtttgatgg tattgattgc cgccagcgcg atgcagggga tgggcaccgg
    15781 cgttggcggc gtaatggcgc gtactttgcc gcgcgattta tatgaacgga cacagttgcg
    15841 ccacgctaac agcctgttaa acatggggat tcttgtcagt ccgctgctcg caccgctaat
    15901 cggcggtctg ctggatacga tgtggaactg gcgtgcctgt tatctctttt tgttggttct
    15961 ttgtgccggt gtgactttca gtatggcccg ctggatgccg gaaacgcgtc cggtcgacgc
    16021 accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg gttttaactg
    16081 ttatttgctg atgctgattg gcggtctggc cgggattgcc gcatttgaag cctgctccgg
    16141 cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt tgtttattct
    16201 gccgattccg gcggcatttt ttggcgcatg gtttgccgga cgtcccaata agcgcttctc
    16261 aacgttgatg tggcagtcgg ttatctgttg cctgctggct ggcttgctga tgtggatccc
    16321 cgactggttt ggcgtgatga atgtctggac gctgctcatt cccgccgcgc tgttcttttt
    16381 cggtgccggg atgctgtttc cactggctac cagcggcgcg atggagccgt tccccttcct
    16441 ggcgggcacc gctggcgcgc tggtcggcgg tctgcaaaac attggttccg gcgtgctggc
    16501 gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctgggattgt tgatgacctt
    16561 aatgggattg ttgatcgtgc tgtgctggct accgctggcg acgcggatgt cgcatcaggg
    16621 gcagcctgtt taagctcacg tcaccgcagc atcgtcatca gctccatggg agaacgatgc
    16681 tgctttatca gatcacgcat cacccgcata tgcggcgcgg agtaagaata aaacgcctga
    16741 tagcccgcac aaagcacgtt gtctggcgtc tcctcccgag cgtgcgggca caggcgatgc
    16801 cagtcgcacg cctggcagcc acgacgcggt tgcgggtttt cgctgccaca ccatttttgt
    16861 agggtggcgt caaaaagctc aatcgaaata cgactgatat ctgcccgtac ccaacactcg
    16921 aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc cgtcagatcg
    16981 ccccggtcat caaattcaag atggggaata agctgtacgg agcggatacc ccgttgtttg
    17041 aaattgtgat aaaaatcggc aggttgcaga gcatccttgt actctacaac cagtttatgc
    17101 ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca ataaatacga
    17161 taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc gcgagggaga
    17221 tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg acatccatta
    17281 aattgcgatt acgggtgtag tgccagagga tcagcgccac aatggcgaga atgccaatcg
    17341 aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac gccatcaacg
    17401 cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt acgctccggc tgcagccctg
    17461 ggtcggcaat gcgtctggct ttgcggctat ccggcataca acaccagtcc cataacgatc
    17521 actgccacga ccatcaatat taaactgatg attaataagc tgttggtata gggcaggtcc
    17581 tctttcaggc gcatcgcttt ttcattgcgt aaccagcgca agtagccata catcgctaac
    17641 ccgccggaaa acagacacaa caacaacgcc agcagttcgc ggatgacggg cgtggcgaaa
    17701 tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc agtacggatc
    17761 cacgccagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc ttctccgagg
    17821 cgggaaattt tcattgttgc tccttgtaaa ctgttcctcg ccgaaaaatt aggccaggaa
    17881 cagcaataat gcatcagaac ttctgttttt tctcttccac cttcacccct tgtgtcggta
    17941 aaccggtgtc atagtcgcgt accactggag aataaccatc ctgtgggcgt ggacgaaacg
    18001 cccccatcca gcgcggttgt gcatctttgc gccacggacg caggttccat tgataactgc
    18061 ggaacggatc gcgaattttg tccatgtaat ccaataaggc gtcatgcatt ttgctgcgaa
    18121 cgtcggcaaa atggatatca tcgatcaggt tatgcatttc atttgggtca ttacggcgat
    18181 cgtaaagttc atcactggtg aagaggttga gcaccagttt aaagtcatcc gttacccagc
    18241 aacgcaccgg aataaaaccg ccaaagctgt catgctcaat ctcgtagcga ttaaattcca
    18301 ccaacacgcc acgtggttct tttacggcaa ggatattttc ccccggcaga atctctggtt
    18361 tttcaatatc tgccagcgcc atcattgtcg gcagtaagtc aatatgactg actggcgtat
    18421 cgacctgtcg ccgctcccct tgcggcgaac ggatgatcag cggaatgcgg gtgatgtcgt
    18481 catacatcgc cgctccttta ctgatcagct tatgtgcgcc catcatttcg ccgtgatcgg
    18541 aggtataaat aacccacgta ttttcacgtt gctctggcgt taaggcattg atgacccgtc
    18601 cgatttggtc atcaacaaag tcattgcagg caaaatagag cggatggtga taaagcccgt
    18661 catcaccgac tggcgatggc atcgcctgcg cccataagcg gtgatgttcc ggtttgttcg
    18721 ccaggtcatc ctctgctttc tcacccagtt cgtagtaaaa atcagtgtat ttctctaaat
    18781 actccaccgg acaggtgaac gggtgatgcg gctcatcata cgaaatcacc atcaggaaag
    18841 gttcgtcggc gcgcgcgggc tgttgcagaa aacccaccgc ccgattgctg atgcggtgcg
    18901 cccaggtgaa ggtttcgtca atatggttcg cctgtaaatc ctcaacgctg tttaggccat
    18961 tgcgccacag gctaatctct ttctccgtca gttcactaag atagttcgcg ccatcgaacc
    19021 agtaatcagc gtcccactcc ggcggacact cgccagtgcc gaaatagtca tgtccatcga
    19081 gatgccattt gccgatgtag caggtgtgat agcccgcatc cttaaagtag cgccccatgg
    19141 tggaaatgtt tttgcccggc gcgacgttgt tggtccacgg gccggactgg ttagcgtaga
    19201 taccggtgaa tagtccggcg cgtgcaggtg tacaaaccgg tgaacaggtg taggcagaat
    19261 taaagcgaat accttctgcc gccagactat caatattttg cgtattcagc ggcttaccgc
    19321 tatagcaacc gaccatattg gtggcctggg tatcggtcat gatgaacaga aaattggggc
    19381 gtttcatagg ctatccttcg catcgggagt aaaggttacg gctgggtcgt gacgatgccg
    19441 ccagctgtca aaaatcaggt agacaatcac tacggcggcg atgaaataac tgattatcgc
    19501 taaccagcgc gtaccgtagc caccgaattc agccagcccg gcatagacgc caatcatcgc
    19561 gaacaggata ccaattgacg caatcttgac gtttttccac ggtttcatgt ccaccgcaaa
    19621 cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca ccagcatcac
    19681 gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt cgaacttcac
    19741 cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt ttgccgccag
    19801 cgccggaatg cgcgggaaga aaaagcccat aatgatgatg gtaaccagcg gcacgttgta
    19861 aataccgttg agctgtttca tccagctata cagcccctgc ggcgcgttgg cgatccacgg
    19921 cgcgaccatc accgaaacga tggcgataaa gaaaccaaat ttgcgcccga cggtgaccag
    19981 ctgttgcggc tcggcattct ggttaatgat gcgccggtaa atacccatac tgaataacgt
    20041 actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata acaccgcacc
    20101 gaagaagccc accagtggca ccggcagaac gttattgacc agcgtcgggt aggccatgtc
    20161 ggctttcggt aaatcctgat acagatgaaa cgcaatcaac cctggcagca ccagtaccag
    20221 cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt ccgccaggct
    20281 ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa aggtgttcac
    20341 cagaatcaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc cgccgactga
    20401 gtttaatttc tcggcatgaa cggtggtgag ttgctcaatg ccctgcataa agctgccctt
    20461 gcccatcgcg atcaggccaa ataccggcac catcaacccg ccaataacca gcccaatacc
    20521 gttaatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat acaaaattcc
    20581 cgccagacca agcaaaataa ccagtaacca gatagccgca ccatcggaaa gttgtagcga
    20641 ctcacccaca tgaaacaggc tgttgagcgc caacgcgccg gaatagagaa caatcggcag
    20701 aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg tcgttttatc
    20761 ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat agcgcggtaa
    20821 aaagattagc gcgaggaaga tcagcgtcac tgcagaggtg acttcccagc ccatcaccga
    20881 catgccgctt ttgtaggcct gcccggaaag gccgaccagt tgttccgtgg aaaggttggt
    20941 tagcattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa gaaaatagcc
    21001 ttgttgcgat ccggtatctg ttttacggac cttccaccag gtgatgaccg ctaccagcag
    21061 cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct tatttatgtt
    21121 tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg cagtcagatt
    21181 cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc aggttgtgag
    21241 cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcgg atgtaaaaaa
    21301 cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca gcggaatttc
    21361 tctgatctcg ctctggcata cctacgccga cgagcattat cgggtgatct ggccgcggga
    21421 caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac aaggatgtgg
    21481 gaaaattctt ttaaagaatg acgaacaaat aactctgcat ggcaactgta ttatattttt
    21541 aaagccaatg gatattcact cttatcactg tgaaggtttg gtctgggaac agtactggat
    21601 ggaatttacc cccaccagta tgatggatat tcccgtcggt cagcaaagcg ttatttataa
    21661 tggcgaagtt tataatcagg aactcaccga agttgctgag ttaattactt caccagaagc
    21721 aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt ggatttgtct
    21781 tatgtacgca gtcggtaaaa aagatccaca acggcggcaa attgaaaaat taattgccac
    21841 tttacatgcc aatctacaac aacgctggag cgtagctgat atggctgcca cgatcccctg
    21901 tagcgaggcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc cgaaagaata
    21961 ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag ggaactcggt
    22021 tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca aagcctttaa
    22081 gcataaattt ggttatgcgc cgtcagctgt gctgaagaat acggacaggt aataaccaga
    22141 atggcgagtg tgtaaatgac tcgatgatgt tcagaaatac caggcggagt accatttacg
    22201 gcgactccac cgggaaaaga gttaaccgat actccagaag agcaccgcca gtaattgggg
    22261 tgtgataatg cgcaggaaca tcactaacgg atagacagtg gcgtaagaga gcgccgccgc
    22321 accgctggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg tcatggaacc
    22381 tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg ccagaatgcc
    22441 aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc ttagcccttc
    22501 gccatccacc agtgtatgga taaaatcgcc gccggatttc aggccaacca ccgagagaaa
    22561 gagcacaatg cctaactcgc gtagcgccag gttagcgctt ggtggcataa accagtacag
    22621 cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggac cgcccgccag
    22681 cccgagtttc aacgccgccg ggaacccagg cacaaagacg ggaatagaac ctaacaatac
    22741 gccaagcccg ataccaataa acactggcag catctgaacc tgttgcagtt tttgctgcgc
    22801 attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca ggttgaggat
    22861 atcgccgaac tgcaggctga tatcgctact tgcaactaat tcaacaccag cacggttcag
    22921 gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac gtttaccgag
    22981 cacgttttca ttggtgacca ccacacgcgc gacgcgcaaa tcagtgcctt tcgtagacag
    23041 cgaggtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg caggctgccc
    23101 caccagatgc agcaaatcgc ccagttgaat aacggtttct ggcgaaggca cttttagggt
    23161 ttcttctcgt ttcaatcgtg agcaaataac tttatcgcca ttgagaatcg gcacatcttt
    23221 aatggctaaa ttatgcaaat taggattctc aactcggatg ttaatcgtcc tgattagcgc
    23281 accgccgttg gttcgcgtgg actcgtgctg ctgggcctca gtctcaacat tgacgcggaa
    23341 aataacccgc agcatccaca tagtgaacaa aatgccgcaa atcccaaatg gatacgccat
    23401 cgcatagctc atccccattt gatcgaccat cgccatcggt gtccccaggt cgcgcaagat
    23461 ctgctgccct gcccccagcg ctggcgtatt ggtgaccgcg ccggaaaaaa tccccagcac
    23521 taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac caccgatgat
    23581 gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg aggcaaagaa
    23641 gcccggccct acctgaatcc cgatggtata aacgaacagg atcaggccaa actcctgaat
    23701 aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat ggccgacgat
    23761 gatcccaccg aacagcacgc cgccaatgcc caatccaaca ccgcgaaact tgacgttgcc
    23821 gataaacaac ccgacgaccg ccaccaaagc cagaatactg accgttaatg ctatatcact
    23881 catcgtccat ttctcttgaa ataacatttt aattgctacg gattatggct gaatcggtga
    23941 agcagatttg ccttatagcg cacattatca cgggaaagaa cgtgccgaaa tatcttaaac
    24001 aggcgaaatt taacgcattg ctgaagaaaa aagacaaaaa aaagccccac cagaatggcg
    24061 gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata gcgatacgct
    24121 gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa ccgtttacga
    24181 aggttgcgcc agagacttcc atattttcag ccagcgtaaa gctcaggcta aaaggctgat
    24241 tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctctggc gtgcctttta
    24301 cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc ctgacggaaa cctgccagcg
    24361 caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc gggaagctct
    24421 ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc cattgacgca
    24481 tcagtgggga taaatcgaag ttacgcatag ccatttctcc ttctaagaag cgagtaagta
    24541 cctgcaaatc cgaagattcg catatgctcc ctgacggcga gcatggaaat gtcaggccgc
    24601 gccaggcggc cttagggaat tagttgattt cgatacggcg cggttttttc gcttccggaa
    24661 tcacgcgttc gagatcgata tacagcagac cattcaccag gttagcacca cgaacatgaa
    24721 tgttctcagc taactggaat ttacgttcaa agttgcgttc agcgatgccc tggtacagat
    24781 aggtgcgctc tttttgttcg tcggcgtgag cacctttcac caccagcaga ttatcctggg
    24841 cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg cgataatggt
    24901 tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg ctctggttgt
    24961 tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc ggggataaat
    25021 caaagttacg cataatcaat agctcctgaa atcagcgaga atctaagacc ttccacaatg
    25081 gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcggctacg atgtaaaaat
    25141 gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag tttatggtct
    25201 attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc acagtgcgac
    25261 gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca acacccacga
    25321 tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat atgcagcgga
    25381 atgacattac tcggaggatg ctccagcgtg atgtcccata ccggggggaa agaaggaacg
    25441 tatcctggca cccgcgccag cgcaaccatg attggcgacg atgaaaccaa ctggggcaca
    25501 aaatcgctgg cgatactcga catgccattt accgcagtac tggacacaat attgctaccg
    25561 tgggatgtgt tccgcaaaga cagctcagtg cgctcgcgcg ttgaaaaaag cgaagccaac
    25621 gcgcaagaaa ccaataacgt cattcccccc gccaaaatgc ctgcaaactg agagctagcg
    25681 gtcagtttcg gtgatccaca actgaccgtc ctccatccac gccaaccatt gaccatccgg
    25741 cgagaaaacc acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac
    25801 ctcgccgctt tgcgcatggg cacaagcaat ccgattatcc agcacaaatc ccagccattc
    25861 gcctgatgga tgccagttaa atgcagactg aatatccgtt ttattatggg ttaactggcg
    25921 tggttcaccg ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat
    25981 taaaaacgca atttgtgttc cttgaggatt acaacgcacc cagtggcgcg gaatgttaac
    26041 caggcccggg taaatacgat ggtgcgtaaa ggttaaacgt cgctgcacga caccacgcgg
    26101 tggcgcaggc agggttgttt ccgttccgct taacggcgca tcacctgcca ctttccagcc
    26161 agcttcatct ttcggcaact cgacaataaa cagctccggc actttctcgc cctttggcga
    26221 aagtgtgtcg ccaataaacg ccagtgcgtg atttcctacc catccttctt cataagcacg
    26281 attgatttca tcactgccag gctgtggcgt gggcgtggtt ttacttacca gcacgcacca
    26341 gtggctaccg ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggtgc
    26401 agcaacaccg acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt
    26461 gtaggtaaag ctcacccttt caccgttcgg gctaaagaca tgtacatgac tgccgccgcg
    26521 cagcgctcct ggagtatatg gcgcggtaat atccattgca tcgagattgc tcaccttgcc
    26581 gctttcaaca atcactccgc gccgatgatg aaagtcgtaa taccatgttt catcaggatt
    26641 ttccgggccg tggatgaaga catacttctc tgacttcgta tgaacggtca ccacgccgac
    26701 gtatgcgccc tgtgacgcgc gatagataac ctcgacctcg ccggtatgaa tattcacacg
    26761 ctcaatggtc tcgccggtaa acgacgcccc agaaggccgc acgtcaaaca ccagccactg
    26821 gctgtcgggc gtccaggtat tggtattggt gagcaggtga ttacggggag caaaggttat
    26881 ctgtttcatg gcgcggtcct gatacaaaac ggttcccgcc atcatacttc acaaggactt
    26941 cactttcaga caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt
    27001 cgacgtggcg attattggcc tcggcccggc gggtcggcgc tggcgcggca gttagcaggt
    27061 aaaatgcagg tgatcgcgct ggataaaaag caccagcatg gtactgaagg tttcagcaaa
    27121 ccttgtggcg gtctgctggc accggacgcg cagcgatctt ttattcgcga tggactgacg
    27181 cttcctgtcg atgtcatcgc caacccgcag attttcagcg tcaaaaccgt cgacgtcgcc
    27241 gcatcgctca cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac
    27301 ttgtggatga aatcgctgat ccccgccagc gttgaggttt accacgacag cctgtgtcga
    27361 aaaatctggc gtgaggatga taaatggcat gtcatttttc gcgcagacgg ctgggagcag
    27421 catattaccg cccgctatct ggtcggtgcc gatggtgcca actcgatggt gcggcgacat
    27481 ctctacccgg atcatcagat tcgtaaatat gtcgccatcc agcagtggtt cgcagagaaa
    27541 catccggtgc cgttctactc ctgcatcttt gataatgcga taactgactg ttactcatgg
    27601 agtatcagca aagacggtta ttttatcttt ggcggtgcct atccgatgaa agacggtcag
    27661 acgcgtttca cgacgctaaa agagaaaatg agcgcctttc agttccagtt tggtaaggcg
    27721 gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt tgtctgcggc
    27781 aagacaacgc ctttctgatt ggcgaagcgg cgggatttat cagcgccagc tcgctggagg
    27841 ggattagcta tgcgctggat agcgcagaga ttctgcgttc ggtattactg aagcagccag
    27901 agaagctcaa taccgcttac aggcgcgcca ctcgcaaact gcgtttaaaa ctcttcggca
    27961 agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg
    28021 gagtggcgca tattccacag ttgaaagatt atccaacgcg cttcacatcg cccaccagca
    28081 ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga
    28141 aaccgtaacc ctgcgccagg tatcccacca ccagcggaac ggtgatgccg cccagaccac
    28201 cggcaaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga
    28261 ccagcgacca ggttatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca
    28321 taatcatcat cggatcgttg gtgtagttcg cacccataat gcaggtggag atcagcaagc
    28381 cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat
    28441 ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca
    28501 taaaacctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg
    28561 ttaagaaaaa ccacagtgta gaagccaccg caaattgccc aagatagaca ccgatcagtt
    28621 tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca
    28681 ccggcgcatc gccatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc
    28741 tgatgccttt ggtcagacgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa
    28801 tgccgatacc accggtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga
    28861 tcagcagcgg agtcagaaac gccagaccga caaactgacc agacgtataa aaaccaacgg
    28921 cagaagcgcg ttcatgttcc gggaaccaac tggttaccat ccggttattg gtcgggaacg
    28981 cgggcgcttc gaaaataccg gttatcgcgc gcagaccaat taatgacatt aagcccgtgg
    29041 caaagccctg gaataacgtc gccactgacc agccaaatat cgcaataaaa taagtcacgc
    29101 gtgaacctac gcgatctaaa aaccaaccac cggggatctg acatagcgta taaagccagg
    29161 cgaaggccga aaatacatag cccatttgcg ctttagtaat accaaactct tcctgaatat
    29221 gggcagaagc cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga
    29281 tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca
    29341 tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac
    29401 agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa
    29461 ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc
    29521 atttttactg aactcaataa ctttagcttc gtcgatttcc acgcctaagc ccggtttcgt
    29581 taatggttta aagaaaccgc cgaccatgct gaagtcctct ttgtttttca caaagtcgag
    29641 taactccgcg cctttgttgt aatgaatgcc catactttgt tcctgaagga cggcgttata
    29701 ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg gacagtgcgg
    29761 cgcaagggtc acgtcatagg cttctgccat tccggcaatt ttgtagcatt cggtaatacc
    29821 gcctgcgtgg gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacacgttt
    29881 aaaatcgaag cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc
    29941 cagtttcggg tagtattcgg cctgttccgc cagcaccggc tcttcaataa acagcggacg
    30001 atacggttcc agctctttaa tcagcacttt cgccatcggc gcgctgacgc gaccatggaa
    30061 atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac
    30121 tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt
    30181 gaaggtatcg aagccgattt cgcgtagcgt tttaatgccg tcgataacat ccgccggacg
    30241 atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg
    30301 ccagaccggc gcattcagca ctttaccttt gatatcccat aacgcctggt cgatcccggc
    30361 gatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca
    30421 taagtcgttg atgcgtgacg ggtcctgacc aatcaaatag tcactcagct cgtggactgc
    30481 cgcttccacc gtgcgagcgc ggccttcaat cacgggctcg ccccaaccga ccacaccttc
    30541 atcggtttca attttcagga acatccagcg gggaggtaac cgatacgtgg taattttggt
    30601 gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcagtc
    30661 cgttctacgg actgcccggc gcgatagaga tcgctgccta agcccgcccc tgcacaacct
    30721 gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg
    30781 tccggtggta ataccgcttt taatgctttg atgtattgcg gaccaaaagc cgatgacgga
    30841 aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgca
    30901 cagccggggc agacggtcat gccgtagccc accgcacggc ggatcacttc actatggata
    30961 ttgggcgtaa cgatgagctg acagcccatt ctggcgagcg catcgacctg ttcaggtttc
    31021 agtaccgtac ctgcgccaat caacgcctta tcgccgtacg catcaacgat ggcgggaatg
    31081 ctctgctccc attgtggaga attcagcggg atttcaaccg catcgaaccc ggcgtcaatc
    31141 accgcgccaa catgtgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc
    31201 gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc
    31261 gcctgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt
    31321 cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg tccacatagt cacgcatact
    31381 ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag
    31441 tgttcccagc acatgcgagg cgcgaacctc aaaaagctgt ggcaatacgg cgggcgcatt
    31501 aaggccatgc tcaagaccag ccgcgaaggc atcggcagcg ttttcctgcg gcggcaaacc
    31561 tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg
    31621 aaaatcgtta atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcatgac
    31681 ataaagagag gaaggagcca gagtgcgcgc gccgatcaat tgtgtttctt cgccgcgcat
    31741 cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc
    31801 aacagacgtt aattgttcgc caatagacga aaaacgggct ggaacggata aatacggcgc
    31861 aactttccag ccgacattgc tgccgaccat tcctgccatt accactggcg tattctcttc
    31921 acgccagccg gtcgtcactt ctgctaacac cgcagccgga gattttccgt tcaggcgcgt
    31981 gacgcctgct tctgattgcc tgctgtccag gcagtggtcg ccctgataaa gccaggcgcg
    32041 cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac
    32101 cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc gccgtcctga
    32161 tgccttatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca
    32221 tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctcagt
    32281 tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct
    32341 tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc
    32401 tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatagc cggctctacc
    32461 aggtttcgca cttcgctcat tgcactgata agccgtgggt catagtcgtt ttccagcacc
    32521 cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggagccac aaacgccccg
    32581 cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacgaaatac ctcacggatg
    32641 atgttgcgcg aggttgcaaa ctcttcacag agttctgcct cagccggaag cggcgagcct
    32701 ggcacgtatt tgccgtgaac gatctgttta cctagcgtaa taacgatgcg atcggtttta
    32761 ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat
    32821 gcataacgcg gcaattttgt agtaccagcg tgatgacgtt cgcgtttgtt gtgcgtctaa
    32881 tgtagtacaa agttatattg ttgtactaca atttagatca caaaaagaac aatcaataag
    32941 aaaatgttct acgtcgggca aaaatctgaa aagggagatc aggcgctaaa caggagggaa
    33001 agaagagtat gctttcaacg gcttagcact cttgtttaaa ggattaatca tgaaattgaa
    33061 ttttaaagga ttttttaagg ctgccggttt gtgttctttg gcatttgcgt tgaccggatg
    33121 tatcacctgg ggattggttt ccaatactgc aagccgtcca catgccgagc aatggcggtc
    33181 agacacgatt aagggtttat cgctggcaga agatagtaac ggtacgaaag gctatgtttt
    33241 tgttggtgaa tcgttggact atctgttgac caccggcggc gatgaggtag ttaacatgct
    33301 gaacgatccg gcaatccatg gtgaaagaat caccgtttct gataacgcga aatttatttt
    33361 aagcagcagt aataagaatt tctctggtgc tattacgctc tattacgact ggaacaacga
    33421 agaagataag gcactggcaa cacaatatgg ttttatttgt gatacccggc gttgtacatg
    33481 gatgctggac ggtttgaaag gctcaatcca ccagaaaaat aaaaaagcgg attactcaaa
    33541 cgtgatggtt ttccatcaac cctttacggt gggattttat gagtacaaag caacagatgg
    33601 cgtcccgcac ggcctcgtta atgcgctgct gccagtcact cttacgctgg atatagtaac
    33661 gtcaccactc cagttcctga ttttatgcac gaccagaaat tgttaatcgc tgcgggcaaa
    33721 cgcattcatg ccgtttgccc gttcactgat taattcagca catacttctc aatagcaaat
    33781 gccacgccat cttcaaggtt agatttggtg acaaagttcg ccacttcttt caccgaagga
    33841 atagcgttat ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc
    33901 tgatcgccaa tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc
    33961 acacccgtac ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc
    34021 agcacggtgt atttttcttt cacttcctgc ggaatacgcg caatggcctg gtcgaggatg
    34081 gcgggttcat caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct
    34141 tcgcagaaca ccagcggaat ggtggcaacg aaagattcat gcaccgtgta gtagctgata
    34201 tctcggttgg cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg
    34261 cgagagagtt tttccaggaa acggtagtcg tcatagctga gagcggtttg cgccacggtg
    34321 ctaccatcag cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc
    34381 tgttccatat gcagctcttt caggtaattg tgaacacctg cataagggcg acccgtcgtt
    34441 agcacgacat tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa
    34501 atggtgtgat cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc
    34561 atgagttccc caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca
    34621 gtacaacacc cgagggaaaa gggggatgca acgcgcattg cgtgctcccc ttttgcttag
    34681 cggaaaagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt
    34741 ggtgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ttctgccagt
    34801 aaactggaag ctgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
    34861 gtgccgagcg ccgattccac ttgctcgccg ctaactgatt gattgctctg ttgattactc
    34921 aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
    34981 atgccgccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc
    35041 agaaaggcgc cgacaacgtc atcaaaaagg cccatgataa tcacctgtaa agcgttacgt
    35101 gttgactcaa aaagtatagt tctgtgggat gataattgcg gattgcagga ataaaaaggg
    35161 cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat
    35221 ccggcctaca aaaacatgca aattcaatat attgcaggag tctcgtaggc ctgataagcg
    35281 tagcgcatca ggcacgctcg cattgttaac gccattaaat atcgatattc gccgctttca
    35341 gagcgttctc ttcaataaac gcacggcgcg gttcaacggc gtcgcccata agcgtagtga
    35401 acaactggtc ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cggctttccg
    35461 ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
    35521 ggatggagag gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg
    35581 ctaccggctg acgacgttcg ccacgttcga taaatgcatc ttcttccagc aagccacgca
    35641 gtttctcacc cagcgtgcag atacgacgat attcgccgcc agtgataaat tcgtgatcca
    35701 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaagtttt
    35761 gctcagcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttatcgttca
    35821 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcgtcagaa aggtcggttt
    35881 ccgtcagcgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
    35941 tgcggttgat cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc
    36001 cagccagcgc cggtgcactg gcgttggtgt gcagcgttgc gccatccagc gcgatagaga
    36061 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgctta cctttcttca
    36121 ctttgtacag cggcggctga gcgatgtaga cgtgaccacg ttcaacgatt tccggcatct
    36181 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
    36241 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
    36301 cacaaccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
    36361 gcgctttctc gacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
    36421 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc cacaaggtac agttcggaaa
    36481 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggccagct aaatccagcg
    36541 cacctttacg gcgggtcatt tcacgcgcgc gacgcgcagc ttcacgggca cgggcagcat
    36601 cgataatttt gccgaccacg attttcgcgt cggttgggtt ttccagcagg tattccgcca
    36661 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
    36721 tggtctgtga ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt
    36781 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
    36841 tgtaggcgtt cagagtacgg gtcatcgccg cacggaaacc tgccaggtga gtaccgccgt
    36901 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
    36961 gcaacgccac ttcgacgcca ataccgtctt tttcggtgga gaagtagaag atattcgggt
    37021 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt
    37081 ggaagtggtc ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga
    37141 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
    37201 tgaaggtttc aaggcttggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
    37261 taaccgccag cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
    37321 cgcgctggat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
    37381 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
    37441 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
    37501 cgcgcccatc atcctgtacg gagacagagt tatcggcgtg aatggtgacg ataatttctt
    37561 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
    37621 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
    37681 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
    37741 gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa
    37801 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
    37861 tgcgtcgctt ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca
    37921 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc
    37981 gccagacgta aggcgcacat cagcagctta agctgcccac gcgataaggt atcttccacc
    38041 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt
    38101 agctggcgat cgcgttcaaa attacgttcc agcacctcag catattctgt ctctttctcc
    38161 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatcg
    38221 gccatatcag ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc
    38281 agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc
    38341 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt
    38401 tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt
    38461 aaagtaaacc cttctggcgt tatcagctgc attggcatca ggtgcgccag ctccgcgact
    38521 ttatgcccgt ctgtaccgtc aatacggact ttgctgtcgc cttgtttgtc tttggttaaa
    38581 ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc
    38641 tcgtggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccgtg gccgagcgta
    38701 tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag
    38761 ccgggagata aggcgagatc cgcggtttca atgttgcgga aatcgcggat caacaagcgg
    38821 gtgagggaca ttacagtctc attggcatga caacataagc cgcgctctgg ctggccgcat
    38881 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
    38941 gtgcgttgag aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc
    39001 tataggtaac gtcgaggatc tcttccgctt cttcctgttc tgggttgttg gcggtgattt
    39061 tcagctggtt ttcgctgaca tacagacgca cgccgcggaa tttctcgtta gagagaattg
    39121 ccgcacgagc aaacgcctgc ttgagcagat cacagccagc ttccagatgt ttgtccgggt
    39181 tcttcggcag aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa
    39241 taaagtcgcc aacgtgggcg cgaatgttgt tgctgccaat ctgcacgcgc agcggattgt
    39301 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat
    39361 ggcttggcaa agattgacca attggcattg aacagactgc cagacggtgg ccgtcggtcg
    39421 ccacggtgcg cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa
    39481 cgtcctgatg cgccatagaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg
    39541 gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatct gccgctggca
    39601 gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgctca ccttccagct
    39661 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
    39721 gaacggtcgt cgcccctggt tcgtgcggct gaaccagcgc aacgcgtgcc accatttcca
    39781 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
    39841 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
    39901 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
    39961 ttagaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttccggcag
    40021 gcatgaagca ccgtcgtgtg gtcacgacca ccaaaggcat cgccaatctc cggaagactg
    40081 tggttagtca gctctttcgc cagcgccatc gccatctggc gcggacgggc caccgagcgg
    40141 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
    40201 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcacg cagcgcctca
    40261 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cgttggcaat gacgcggttc
    40321 agcgcgcctt ccaactcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
    40381 tcgcccggca aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacgcgggtt
    40441 tccagctctg gcggttcgat cgccacagtc agtccccagc cgaagcggga tttcaaacga
    40501 tcctcaacgc cgttgatctc tttcggatag cgatcggagg tgagaatgat ctgttgatta
    40561 ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca
    40621 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
    40681 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
    40741 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg
    40801 tgagttttac ccaaacccgt gccgccataa aggaacaacg ggttataagc accgccaggg
    40861 ttatctgcca cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
    40921 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggagccggg
    40981 acgttatccc aacccgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
    41041 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
    41101 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
    41161 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
    41221 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
    41281 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
    41341 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
    41401 cgtacgtcga aaagaccctg catgagggtg acgcacgaac cgctgtctgc ggttatatgc
    41461 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
    41521 tcttctgttt ctcacagatt ttcccgattt atccacagga tgatccagaa ctcgctaagt
    41581 gtaaacgatc ctgccgcggg ccgggcacga tttacgccgc atattggaaa atttaatgac
    41641 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
    41701 ccgtcagccc gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggcc
    41761 gtacgttttc cccgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
    41821 ccggagtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
    41881 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
    41941 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
    42001 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
    42061 gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt
    42121 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
    42181 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
    42241 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
    42301 cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
    42361 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
    42421 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
    42481 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
    42541 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
    42601 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
    42661 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
    42721 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
    42781 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
    42841 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
    42901 caacacccgt ggtggtgatg ttgagcaagc cctgctgcct gcttacccga aagagctgaa
    42961 ctctacccag ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag
    43021 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
    43081 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
    43141 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
    43201 cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac
    43261 ctttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
    43321 cttcgcactg cacaccttcc gtggtgcggc gtactccacg cctgacgaga agtacgagaa
    43381 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
    43441 ggtggcaatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
    43501 caacttctat accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc
    43561 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
    43621 aattcaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
    43681 gttgtggttc atctctcagc cactgttcaa actgctgaaa tggatccata gctttgtggg
    43741 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
    43801 gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc
    43861 aatgcgtgag cgtctgggcg atgacaaaca gcgcatcagc caggaaatga tggcgctgta
    43921 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
    43981 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
    44041 actgtggatc cacgacctgt ctgcacagga cccgtactac atcctgccga tcctgatggg
    44101 cgtaacgatg ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca
    44161 gaagatcatg acctttatgc cggtcatctt cactgtgttc ttcctgtggt tcccgtcagg
    44221 tctggtgctg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    44281 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    44341 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    44401 gcaccgcaca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    44461 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    44521 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    44581 tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc
    44641 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    44701 gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc
    44761 gggtgagttt tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc
    44821 gattgcggat cttatcgacg ccagctcgga acaggcggcc cgttcggcgc taaactcgct
    44881 gcaaggcgcg ttctccgcac gggtcaacca tctggtggaa gccctcaccc acttgcgcat
    44941 ttacgtcgaa gcggcgattg atttccccga tgaagagatc gatttcctct ccgacggcaa
    45001 aattgaagcc cagctcaata acgtaattgc cgatctcgac gcggtacgcg ccgaagctcg
    45061 tcagggtagt ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg
    45121 taaatcgagc ctgctgaacg cgctggcggg ccgtgaagca gcaatcgtaa ccgatatcgc
    45181 cggaaccacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgttgcatat
    45241 catcgatacc gccgggctac gtgaagccag tgacgaagtt gaacgtattg gtatcgagcg
    45301 cgcatggcag gaaattgaac aggcagaccg cgtgctgttt atggtcgatg gcaccacgac
    45361 agacgccgtt gatccggcag agatctggcc ggaattcatc gcccgtctgc cagcgaaact
    45421 gccgatcacc gtggtgcgca ataaagccga tatcaccggc gaaacgttgg ggatgagcga
    45481 agtgaacggt catgcgttaa ttcgtctttc ggcgcggact ggcgaaggcg tggatgtact
    45541 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc
    45601 gcgtcgtcgt cacctacagg cgctggaaca ggcggcagag catttgcagc aaggtaaagc
    45661 gcaactgtta ggtgcctggg cgggtgaact gctggcggaa gagttgcgcc tggcgcagca
    45721 gaacttaagc gagatcaccg gggaatttac ttcagacgac ctactggggc ggattttctc
    45781 cagcttctgt atcggtaagt aaccgcgctt gcgaagccgc attctgactg tcagatgcgg
    45841 cttcgcttca ttgttagcac tcctgttatt cctcaaccct tttttaaaca ttaaaattct
    45901 tacgtaattt ataatcttta aaaaagatat ttaatattgc gtcccgaacg attgtgattc
    45961 gattcacatt taaacaattt caga
//
LOCUS       DAZFRE010000028        44456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFRE010000028 DAZFRE010000000
VERSION     DAZFRE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.028"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..823
                     /locus_tag="ACHKUI_001896"
                     /pseudo
     CDS             1..823
                     /locus_tag="ACHKUI_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764304.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(883..1374)
                     /gene="yajQ"
                     /locus_tag="ACHKUI_001897"
     CDS             complement(883..1374)
                     /gene="yajQ"
                     /locus_tag="ACHKUI_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6598094.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1542..2453
                     /gene="panE"
                     /locus_tag="ACHKUI_001898"
     CDS             1542..2453
                     /gene="panE"
                     /locus_tag="ACHKUI_001898"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6598095.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTATWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2416..3006
                     /gene="yajL"
                     /locus_tag="ACHKUI_001899"
     CDS             2416..3006
                     /gene="yajL"
                     /locus_tag="ACHKUI_001899"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6598096.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3060..4508)
                     /gene="thiI"
                     /locus_tag="ACHKUI_001900"
     CDS             complement(3060..4508)
                     /gene="thiI"
                     /locus_tag="ACHKUI_001900"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6598097.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4714..4956
                     /gene="xseB"
                     /locus_tag="ACHKUI_001901"
     CDS             4714..4956
                     /gene="xseB"
                     /locus_tag="ACHKUI_001901"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6598098.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4956..5855
                     /gene="ispA"
                     /locus_tag="ACHKUI_001902"
     CDS             4956..5855
                     /gene="ispA"
                     /locus_tag="ACHKUI_001902"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6598099.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            5880..7742
                     /gene="dxs"
                     /locus_tag="ACHKUI_001903"
     CDS             5880..7742
                     /gene="dxs"
                     /locus_tag="ACHKUI_001903"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6598100.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLQKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            7797..8771
                     /gene="yajO"
                     /locus_tag="ACHKUI_001904"
     CDS             7797..8771
                     /gene="yajO"
                     /locus_tag="ACHKUI_001904"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6598101.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(8825..9343)
                     /gene="pgpA"
                     /locus_tag="ACHKUI_001905"
     CDS             complement(8825..9343)
                     /gene="pgpA"
                     /locus_tag="ACHKUI_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6598102.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9321..10298)
                     /gene="thiL"
                     /locus_tag="ACHKUI_001906"
     CDS             complement(9321..10298)
                     /gene="thiL"
                     /locus_tag="ACHKUI_001906"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6598103.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSNSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(10376..10795)
                     /gene="nusB"
                     /locus_tag="ACHKUI_001907"
     CDS             complement(10376..10795)
                     /gene="nusB"
                     /locus_tag="ACHKUI_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6598104.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10815..11285)
                     /gene="ribE"
                     /locus_tag="ACHKUI_001908"
     CDS             complement(10815..11285)
                     /gene="ribE"
                     /locus_tag="ACHKUI_001908"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6598105.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11374..12477)
                     /gene="ribD"
                     /locus_tag="ACHKUI_001909"
     CDS             complement(11374..12477)
                     /gene="ribD"
                     /locus_tag="ACHKUI_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6598106.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12481..12930)
                     /gene="nrdR"
                     /locus_tag="ACHKUI_001910"
     CDS             complement(12481..12930)
                     /gene="nrdR"
                     /locus_tag="ACHKUI_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6598107.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13081..13620
                     /gene="yajI"
                     /locus_tag="ACHKUI_001911"
     CDS             13081..13620
                     /gene="yajI"
                     /locus_tag="ACHKUI_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6598108.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQIKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            13919..14803
                     /gene="tsx"
                     /locus_tag="ACHKUI_001912"
     CDS             13919..14803
                     /gene="tsx"
                     /locus_tag="ACHKUI_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6598109.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14841..15188)
                     /gene="yajD"
                     /locus_tag="ACHKUI_001913"
     CDS             complement(14841..15188)
                     /gene="yajD"
                     /locus_tag="ACHKUI_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6598110.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15318..16289)
                     /gene="secF"
                     /locus_tag="ACHKUI_001914"
     CDS             complement(15318..16289)
                     /gene="secF"
                     /locus_tag="ACHKUI_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6598111.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16300..18147)
                     /gene="secD"
                     /locus_tag="ACHKUI_001915"
     CDS             complement(16300..18147)
                     /gene="secD"
                     /locus_tag="ACHKUI_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6598112.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18175..18507)
                     /gene="yajC"
                     /locus_tag="ACHKUI_001916"
     CDS             complement(18175..18507)
                     /gene="yajC"
                     /locus_tag="ACHKUI_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6598113.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18530..19657)
                     /gene="tgt"
                     /locus_tag="ACHKUI_001917"
     CDS             complement(18530..19657)
                     /gene="tgt"
                     /locus_tag="ACHKUI_001917"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6598114.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19712..20782)
                     /gene="queA"
                     /locus_tag="ACHKUI_001918"
     CDS             complement(19712..20782)
                     /gene="queA"
                     /locus_tag="ACHKUI_001918"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6598115.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20875..21456
                     /gene="acpH"
                     /locus_tag="ACHKUI_001919"
     CDS             20875..21456
                     /gene="acpH"
                     /locus_tag="ACHKUI_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6598116.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSERWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(21461..23278)
                     /gene="malZ"
                     /locus_tag="ACHKUI_001920"
     CDS             complement(21461..23278)
                     /gene="malZ"
                     /locus_tag="ACHKUI_001920"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6598117.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPVVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQEHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGVRNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23434..24807)
                     /gene="proY"
                     /locus_tag="ACHKUI_001921"
     CDS             complement(23434..24807)
                     /gene="proY"
                     /locus_tag="ACHKUI_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6598118.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24883..26202)
                     /gene="brnQ"
                     /locus_tag="ACHKUI_001922"
     CDS             complement(24883..26202)
                     /gene="brnQ"
                     /locus_tag="ACHKUI_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6598119.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKTSAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26609..27904)
                     /gene="phoR"
                     /locus_tag="ACHKUI_001923"
     CDS             complement(26609..27904)
                     /gene="phoR"
                     /locus_tag="ACHKUI_001923"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6598120.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27962..28651)
                     /gene="phoB"
                     /locus_tag="ACHKUI_001924"
     CDS             complement(27962..28651)
                     /gene="phoB"
                     /locus_tag="ACHKUI_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6598121.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28841..30043
                     /gene="sbcD"
                     /locus_tag="ACHKUI_001925"
     CDS             28841..30043
                     /gene="sbcD"
                     /locus_tag="ACHKUI_001925"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6598122.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIITTGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            30040..33183
                     /gene="sbcC"
                     /locus_tag="ACHKUI_001926"
     CDS             30040..33183
                     /gene="sbcC"
                     /locus_tag="ACHKUI_001926"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6598123.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     EKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALASYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAEALALHQQHRPDGLDLSVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            33309..34493
                     /gene="araJ"
                     /locus_tag="ACHKUI_001927"
     CDS             33309..34493
                     /gene="araJ"
                     /locus_tag="ACHKUI_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6598124.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(34762..35670)
                     /gene="mak"
                     /locus_tag="ACHKUI_001928"
     CDS             complement(34762..35670)
                     /gene="mak"
                     /locus_tag="ACHKUI_001928"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6598125.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            35795..36706
                     /gene="rdgC"
                     /locus_tag="ACHKUI_001929"
     CDS             35795..36706
                     /gene="rdgC"
                     /locus_tag="ACHKUI_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6598126.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36787..36867)
                     /locus_tag="ACHKUI_001930"
     CDS             complement(36787..36867)
                     /locus_tag="ACHKUI_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598127.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(36864..37202)
                     /locus_tag="ACHKUI_001931"
                     /pseudo
     CDS             complement(36864..37202)
                     /locus_tag="ACHKUI_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37350..37634)
                     /gene="ppnP"
                     /locus_tag="ACHKUI_001932"
     CDS             complement(37350..37634)
                     /gene="ppnP"
                     /locus_tag="ACHKUI_001932"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6598128.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSKFHLQVAEPTSYLCRYL"
     gene            complement(37706..38383)
                     /gene="aroM"
                     /locus_tag="ACHKUI_001933"
     CDS             complement(37706..38383)
                     /gene="aroM"
                     /locus_tag="ACHKUI_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6598129.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(38641..38832)
                     /gene="yaiA"
                     /locus_tag="ACHKUI_001934"
     CDS             complement(38641..38832)
                     /gene="yaiA"
                     /locus_tag="ACHKUI_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6598130.1"
                     /translation="MPTKPPYPREAYIVTIDKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(38882..39406)
                     /gene="aroL"
                     /locus_tag="ACHKUI_001935"
     CDS             complement(38882..39406)
                     /gene="aroL"
                     /locus_tag="ACHKUI_001935"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6598131.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDLWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39589..40047)
                     /gene="yaiI"
                     /locus_tag="ACHKUI_001936"
     CDS             complement(39589..40047)
                     /gene="yaiI"
                     /locus_tag="ACHKUI_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6598132.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40167..40976
                     /gene="proC"
                     /locus_tag="ACHKUI_001937"
     CDS             40167..40976
                     /gene="proC"
                     /locus_tag="ACHKUI_001937"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6598133.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHSVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(40993..42108)
                     /gene="adrA"
                     /locus_tag="ACHKUI_001938"
                     /gene_synonym="dgcC"
     CDS             complement(40993..42108)
                     /gene="adrA"
                     /locus_tag="ACHKUI_001938"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6598134.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(42210..42530)
                     /gene="psiF"
                     /locus_tag="ACHKUI_001939"
     CDS             complement(42210..42530)
                     /gene="psiF"
                     /locus_tag="ACHKUI_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6598135.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(42649..44064)
                     /gene="phoA"
                     /locus_tag="ACHKUI_001940"
     CDS             complement(42649..44064)
                     /gene="phoA"
                     /locus_tag="ACHKUI_001940"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6598136.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSR
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(44165..44425)
                     /gene="iraP"
                     /locus_tag="ACHKUI_001941"
     CDS             complement(44165..44425)
                     /gene="iraP"
                     /locus_tag="ACHKUI_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6598137.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        11118 a        11912 c        11078 g        10348 t
ORIGIN      
        1 aacctttgcc ttttccccat caacaagaag tgaattaagc ctcattaatt tattaaattc
       61 attaaatgag gtggaattga tgaaaaaacg tctggtgctt ctgatgacag caccagaata
      121 cacttgttat gatagtcagt attgttatga agacaaagtc agcgcattaa aaaccctgca
      181 tagtcatcag gataatgagc taatggacaa atatttcacg actcttctta aagagatcag
      241 taatcaacca ttagaagact gggatataat aataagtaat accggtgaat aatttagtga
      301 ggtgggacta accaaatatg ctcaacatct cgcatctttc gctgaagaat tttatataaa
      361 agagcaaaaa tcacattctg aaagtgaaat gagaggacta ttttcatcac ttgccgaaat
      421 atatagtttt ggtaacgata ctgccagcgc aaaccgagtg ttgcatcaac atgtccctgc
      481 cttcaataca gatcatatga ttcaatatta tataaatgcc aaagaatgga acaatgccag
      541 ggaattattg ataaaagagg aaatgttggg tatgcataat ctgatattgt tggagaacat
      601 ttgtatgcag aaaactgcac aatgcatggc acacattacc tttacgctca acaaattgac
      661 aacacaacct gcaataacaa aaaaagatag cgtgggtaat gagcaacttt atcagattgg
      721 caaaatctac cataagctgg gagttaaacc agagccaaag cagcaggctt taattcagtc
      781 gctttatgac caggcatcag gaagcacctc ttcaactcaa taaacacatg gtcaaaaggg
      841 ccaccacggt ggccctgaca atcaatagct atatccagaa agttaatcgc ggaagttttt
      901 gaactggaac ggctgaccca gatcgccacc acgtaccatc gccatgacag cctgtaaatc
      961 atcacgagat ttacccgtta cgcggatctc atcgccctga atttgcgcct gcactttcag
     1021 tttgctgtct ttgatcattt tgacgatttt cttctgagtc gcgctctcaa tgccctgttt
     1081 cagtttcgct tccacaaacc aggttttacc gctatgaacg atattttccg gtacatccag
     1141 tgaactgcct tcaatgccgc gcttcagcaa cttggcacgc agaatatcca gcaactgatt
     1201 gacctggaag tcggactcgc tcaacacttt gatggttttg ctggcgtcat tcagctcaaa
     1261 tgaggcttca acgttacgga agtcaaaacg ggactccact tcgcggctcg cgttatcgac
     1321 cgcgttacgt gcttcctgaa gatcaacttc agagacaata tcgaaagatg gcatcttttc
     1381 ctctcccttc atttttgatg cgaagcataa tacctgcaaa gttaaataac caacagtcct
     1441 gcaccaggac gtatccctta attgtgcata gtcgcaacag catgcacaat taagggatag
     1501 ccttattgaa cacccggagt ggttgcgggt gaggaggaac aatgaaaatt accgtattgg
     1561 gatgcggtgc cttagggcaa ttatggctta cagcactttg taaacagggt cacgacgttc
     1621 agggctggct gcgcgtaccg caaccttatt gtagtgtgaa tctggttgag acagatggtt
     1681 ctatatttaa cgaatcgctg accgccaacg atcccgattt tctcgccacc agcgatctgc
     1741 tcctggtgac gctgaaagca tggcaggttt ccgatgccgt caaaagcctc gcgtccacac
     1801 tgcctgtaac cacgccaata ctgttaattc acaacggcat gggcaccatc gaagagttgc
     1861 aaaacattca gcagccatta ctgatgggca ccaccaccca tgccgcccgc cgcgacggca
     1921 atgtcattat tcatgtggca aacggtatca cgcatattgg tccggcacgg caacaggacg
     1981 gcgattacag ttatctggcg gatattttgc aaaccgtatt gcctgacgtc gcgtggcata
     2041 acaatattcg cgccgagctg tggcgcaagc tggcagtcaa ctgtgtgatt aatccactga
     2101 ccgccacctg gaattgcccg aatggtgaat tacgtcatca tccgcaagaa attatgcaga
     2161 tatgcgaaga agtcgcggca gtgatcgaac gcgaagggca tcatacttca gcagaagatt
     2221 tgcgtgatta cgtgatgcag gtgattgatg caacagcgga aaatatctcg tcgatgttgc
     2281 aggatatccg cgcgctgcgc cacaccgaaa tcgactatat caatggtttt ctcttacgcc
     2341 gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa
     2401 gaaaggagag tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag
     2461 actgaagccg tcaccactat cgatctgctg gttcgcggcg gtatcaaagt caccactgcc
     2521 agcgtcgcca gcgatggtaa cctggcgatt acctgctcgc gcggcgtgaa gctgctggcg
     2581 gatgcgccgc tggttgaagt ggctgatggc gaatatgacg tgattgtgct gcctggtggc
     2641 atcaaaggcg cagagtgttt tcgcgatagc accctgctgg ttgaaaccgt taaacagttc
     2701 caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt gctggtcccg
     2761 cacgatatct tcccgattgg caatatgacc ggcttcccga cgctgaaaga caaaataccc
     2821 gccgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc
     2881 caggggccag gtacagctat cgactttggt ctgaaaatta tcgacctgct ggttgggcgc
     2941 gagaaagccc atgaagtggc atcacaactg gtgatggcgg cagggattta taattattac
     3001 gagtagtgtc ggatgcggta aacgtcgcat ctgaccagat gcgacgttac aaccacatat
     3061 tacgggcgat acaccttcac attgttaaag ccctgctcgc gcagatagag cgcctgcagg
     3121 cggctcatca ccccgcgctc acaccacaac agccaggttt tgctctggtc gagatcgcca
     3181 aatttggtgc tcagtttata gaacggcaga gaaaccacat caatcccttc gactttcagt
     3241 ggcttatctt cctgttcatc gatagaacgg atatcgagga tcacgtcgtt cgggccgaag
     3301 ccattgacgg tttccacttc caccacttcc tgctcggtct gctgggcgat ttcgcggata
     3361 tcaacgttat tcgcttcctc caccacttta tcgagaatgc tgaagtcgaa cttctcttct
     3421 tccgcttcaa tcttcgattt aaccgctttc accgtcgggc ttttggaaat cacgccgcaa
     3481 tattccggca tcgtgcgggc aaagtcttcg gtgccaatct ggcgagccag gttgatgatg
     3541 tgctctttgt cgtaagagat cagcggacgc aggatcagcg tatcggagac gttatcaatc
     3601 aggcgtaggt tggtcagcgt ctggctggac acctggccga gcgcttcacc ggtaaccagc
     3661 gcctgtacgc cgtaacgttc tgccactttg gacgcggcac gcaccatcat acgtttgagg
     3721 ataacgccca tctgaccgtc gtcgattttc tcgagaattt ccccgacgac cggttcgaaa
     3781 ttaatagcga caaaacgcac gcggtgggag ctgccaaagc ggttccacag ataatgcgcc
     3841 acctgacgta cgccaatttc atgcgccgcg ccgccgaggt taaagaagca gtaatgcacg
     3901 cggcagccgc gacgcatcaa catataactg gaaacaccgg agtcaaaacc accggaaatg
     3961 agcgacaaca catcttcctg ggtgccgatc gggaaaccgc caataccttc gtagcggcct
     4021 ttaatcagca gcagacgatc gtcttccact tccagatgga cagtcacttc cggattggtc
     4081 agcttcacgc gcgcggattc aatatgctga tttaaaccgc cgccgacgta acgttccaca
     4141 tcaatcgagc taaaatcatg tttgccgcgg cgcttcacgc gtacgcagaa ggttttgcct
     4201 tccagctgat cgcgatattg aaccaacgct ttctcgaaaa tatcgtgcat gtctgtaaac
     4261 ggcacgtctt cgacttcgag aatatggtgg atacccggaa tacgggtcag ggcgtcgcga
     4321 atagccagac gctggttttc atcttttgcg cgaacttcga tgttatccca gtggcggacg
     4381 acagcgagcg tctcatcata gtgctttaaa acgttacgaa tgttcccggt aaggattttt
     4441 ataaagcgca agcgcacaga ttggcttttg atggtgattt ccgggaacaa tttaatgata
     4501 aacttcatgg cggcaatggt tcgttggcaa gccttaagcg acttgtatag ggaaaaatac
     4561 agcagcccac acctgcggct gcatccaggc gcggaagtat accactaaca tcgctttgct
     4621 gcgcacatca ccttaccatt gcgcgttatt tgctatttgc cctgagtccg ttaccatgac
     4681 ggggcgaaaa atattgagag tcagacattc attatgccga agaaaaatga ggctcccgcc
     4741 agctttgaaa aggcgctgag cgagctggaa cagattgtaa cccgtctgga aagtggcgac
     4801 ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg tgcagctggc acgtcagggg
     4861 caggccaaat tacaacaagc cgaacagcgc gtacaaattc tgctgtctga caatgaagac
     4921 gcctctctaa ccccttttac accggacaat gagtaatgga ctttccgcag caactcgaag
     4981 cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga
     5041 acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgcctgcgac
     5101 ctttcctggt ttatgccact ggtcatatgt tcggcgttag cacaaacaca ctggacgcac
     5161 ccgctgccgc cgtagagtgt atccacgctt actcattaat tcatgatgat ttaccggcga
     5221 tggatgatga cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa
     5281 acgcgattct cgctggtgac gctttacaaa cgctggcgtt ctcgattcta agcgatgccg
     5341 atatgccgga agtgtcagat cgcgacagaa tttcgatgat ttctgagctg gcgagcgcca
     5401 gcggtattgc cggaatgtgc ggtggtcaag cattagattt agacgctgaa ggcaaacacg
     5461 tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg
     5521 ccgttcgcct tggtgcatta agcgccggag ataaagggcg tcgtgctctg ccagtactcg
     5581 acaagtacgc agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg
     5641 taggagatac tgcaacgttg ggaaaacgcc agggtgccga tcagcaactt ggtaaaagta
     5701 cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg
     5761 atgcccgtca gtcgctgaaa caactgactg aacagtcact cgatacctcg gcactggaag
     5821 cgctagcgga ctacatcatc cagcgtaata aataaacaat aagtattaat aggcccctga
     5881 tgagttttga tattgccaaa tacccgaccc tggcactggt cgactccacc caggagttac
     5941 gactgttgcc gaaagagagt ttacagaaac tctgcgacga actgcgccgc tatttactcg
     6001 acagcgtgag ccgttccagc gggcacttcg cctccgggct gggcacggtc gaactgaccg
     6061 tggcgctgca ctatgtctac aataccccgt ttgaccagtt aatctgggat gtggggcatc
     6121 aggcttatcc gcataaaatt ttgaccgggc gccgcgacaa aatcggcacc atccgtcaga
     6181 aaggtggcct gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg
     6241 ggcattcatc aacctccatc agtgccggaa ttgggatcgc agttgctgcc gagaaagaag
     6301 gcaaaaatcg ccgcaccgtc tgtgtcattg gcgatggcgc gattactgct ggcatggcgt
     6361 ttgaagcgat gaatcacgcg ggcgatatcc gtcctgatat gctggtggtt ctcaacgaca
     6421 atgaaatgtc gatttccgaa aatgttggcg cgctcaacaa ccatctggcg cagctgcttt
     6481 ccggtaagct ttactcttca ctgcgcgaag gcggaaaaaa agttttctct ggcgtgccac
     6541 ccattaaaga gctgctcaaa cgtaccgaag aacatattaa aggcatggta gtgcctggca
     6601 cgttgtttga agagctgggc tttaactaca tcggcccggt ggacggtcac gatgtgctgg
     6661 ggcttatcac cacgcttaag aacatgcgcg acctgaaagg cccgcagttc ctgcatatca
     6721 tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga cccaatcacc ttccacgccg
     6781 tgcctaaatt tgatccctcc agcggttgtc tgccgaaaag tagcggcggt ttaccaagct
     6841 attcaaaaat ctttggcgac tggttgtgcg aaaccgcagc gaaagataac aagctgatgg
     6901 cgattactcc ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt aaattcccgg
     6961 atcgctactt cgacgtggca attgccgagc aacacgcagt gacctttgct gcgggtctgg
     7021 cgattggagg ctacaaaccc attgttgcga tctactccac cttcctgcaa cgcgcctatg
     7081 atcaggtgct gcatgacgtg gcgattcaaa agcttccggt cctgttcgcc atcgaccgtg
     7141 cgggcattgt tggtgctgac ggtcaaaccc accagggcgc ttttgatctc tcttacctgc
     7201 gctgcatacc ggaaatggtc attatgaccc cgagcgatga aaacgaatgt cgccagatgc
     7261 tctataccgg ctatcactat aacgatggcc cgtccgcggt gcgctacccg cgcggcaacg
     7321 cagttggcgt ggaactgacg ccactggaaa aactgccaat tggcaaaggc attgtgaagc
     7381 gtcgtggtga gaaactggcg atccttaact ttggtacgct gatgccagaa gcggcgaaag
     7441 tcgctgaatc gctgaacgct acgctggtcg atatgcgttt cgtgaaaccg cttgatgaag
     7501 cgttaattct ggaaatggcc gccagccatg aagcgctggt taccgtagaa gaaaacgcca
     7561 ttatgggtgg tgcaggcagc ggcgtgaacg aagtgctgat ggcccatcgt aaaccagtac
     7621 ccgtgctgaa cattggtctg ccggacttct ttattccaca aggaactcag gaagaaatgc
     7681 gcgccgaact cggcctcgat gccaccggta tggaagccaa aatcaaagcc tggctggcat
     7741 aatccctact ctactcctgc tatgcttaag aaattattca tggcaggagt ggcagcatgc
     7801 aatacaaccc cttaggaaaa accgaccttc gtgtttcccg actttgcctc ggctgtatga
     7861 cctttggcga gccagatcgc ggtaatcacg catggacact gccggaagag agcagccgtc
     7921 ccatcattaa acgtgcgctg gaaggcggca taaatttctt tgacaccgcc aatagctatt
     7981 ccgatggcag cagcgaagag atcgtcggtc gcgcactgcg ggatttcgcc cgtcgtgaag
     8041 acgtggtcgt tgcgaccaaa gtgttccatc gcgttggtga tttaccggaa ggattatccc
     8101 gtgcacaaat tttgcgctct atcgacgaca gcctgctccg tctcggcatg gattatgtcg
     8161 atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg ctggaagccc
     8221 tgaacgacgt ggtaaaagcc ggaaaagcgc gttatatcgg cgcgtcatcc atgcacgctt
     8281 cgcagtttgc tcaagcactg gaactacaaa aacagcacgg ctgggcgcag tttgtcagta
     8341 tgcaggatca ctacaatctg atttatcgag aagaagagcg cgagatgctg ccactgtgtt
     8401 atcaggaggg cgtggcggtg attccatgga gcccgctggc gcggggccgt ctgacgcgtc
     8461 cgtggggaga aactaccgca cgactggtgt ctgatgaggt ggggaaaaat ctctataaag
     8521 aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt gaagaactcg
     8581 gtgcaacacg agcacaagtt gcgctggcct ggttattgag taaaccgggc attgccgcac
     8641 cgattatcgg aacttctcgg gaggaacagc ttgatgaact gctaaacgcg gtggatatca
     8701 ctttgaagcc agaacagatt gccgaactgg aaacgccgta taaaccgcat cctgtcgtag
     8761 gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc
     8821 acaactacga cagaataccc agtggccagt gatgaccgat aaaatacagg atgccagcgg
     8881 agatcacccc ggcgacaata tcatctatca tgattcccat gccgccatgt acattgcggt
     8941 caaaccagcg gatcggccac ggtttccaca tatcgaggat acggaaaatc acaaacccgg
     9001 ctgcgaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac ataccgataa
     9061 attcgtccca gacaatgctg ccatgatcgt gcacgcccat gtctttcgcc gtttgatgac
     9121 aaagatagac gccgatacag atccccagca ttaccaccag cgaatagagc tgccagggca
     9181 aaaaagtcat cagataccag aagggaatcg ccgccagcga ccccatcgtg ccaggaacga
     9241 tcgggcttaa tccacttccg aatccgacag caagcagatg ccacggatta ctcattttca
     9301 ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt catatccttt ccagtctaac
     9361 gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt catttgcccg
     9421 atacaggtaa acggtacacc caggtgtccg agagccacat ccagcgcgcc acggttcagt
     9481 tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc
     9541 tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatgc caggtcaata
     9601 cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag accgtcagag
     9661 agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgac
     9721 ggacggagat gacgtttgat caagtagtcc gcatctttag catcggcaac ctgcaaacgg
     9781 ttttgcaaaa tcgccagccc ggcagcgcta tcgcccggtg taccggtcac atagatccag
     9841 tcacccggtt tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa gccgtggata
     9901 cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg catatcgtaa
     9961 taattgagaa gatcaaacaa gctgttgctg aaggactcaa gccacgcttc gtctacgtcc
    10021 ggtaaggtta atgccaacgt cagccaggcc ggatcggccc ccatcgctgc cagatcgctt
    10081 aggttcaccg ccagtgcttt ataagccaga tcagcaggat cgatatcagg gaggaagtgg
    10141 ttgcccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg gatattgaga
    10201 agagcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct tacacggtca
    10261 aaataacggg caatcaggga gaactcgcca catgccatac gttacgcctc agcagataaa
    10321 aaagaaaagg ccggcgatca tggaacggtc ttccgtgaat ctaccggcct ggatattact
    10381 ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg aacttatggc
    10441 tgtcttctgc gccgaaagat ttcgccagtt cgatcgcttc gttaatggcc actttgtatg
    10501 gcacatcgct acgtttagac agctcgtaca gcgcaatgcg cagtactgct ttttctacct
    10561 ggcccagttc ttccagcaga cgggacaggt acggcttcat cagtccgtcg aggtatgcgg
    10621 tgttagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg tcttttacat
    10681 cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac aactgccagg
    10741 agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt ttcacggatt
    10801 tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc aagcgcggtc
    10861 agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat cgcttgttca
    10921 atgctttcag tggtcagaac accaaaagca accggaattt cgctgtcctg ggcaacatgc
    10981 gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc accacgaata
    11041 accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc
    11101 agcggcagct cataggcacc tggcacccaa acaacggtaa tgttttcatc ttttacctga
    11161 ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat aaagttgttg
    11221 aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc ttcaataatg
    11281 ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt tagcataata
    11341 tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat gcaggcaaac
    11401 atccgggcct acatgacgta tctctttgaa tttaaattgg ggagcgtcgg ctaatttctc
    11461 aagccctggc agcgtgcata atccacgggc gtcgttgcct aatagtttag gtgcgatata
    11521 gacaatcagc tcatcgacta aacccgcctg tagcaatgcg ccagcgagcg ttggccccgc
    11581 ttccacccag atgctgttaa tctgctgttt acccagttgc atcattagta caaccagatc
    11641 caggtgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc actcacgaga
    11701 atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc gatgttccgg
    11761 cgtcacgcga ttttggctat caatcacaat acgtaccggc tggcggagat tttgttgcgg
    11821 gtagagcccc tgagtttgtt catccagttc agaccaacgc actgttaagg ctggatcatc
    11881 cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgcgcgcgca gtcgttgtac
    11941 gtcgcgtcgc gcctgaggcg aagtgatcca ctggctttcg ccactcgcca tcgccgtgcg
    12001 accatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgttt
    12061 gagaaagcct ttattcaatt gctcagcttc actcatcatc agcccgtggc tgacgtcaat
    12121 gccagcctgt tgcagacggt aaagtccacg tccagcgacc tgcggattgg ggtcttgcat
    12181 tgcagcaacc acgcgcgcca cgcccgcagc aattaatgca tcacaacacg gtggcgtacg
    12241 accatgatgg ctacagggtt cgagtgtgac atacgccgtt gcgcctttgg ctttttcgcc
    12301 ggccatgcgc aacgcgtgca cctcggcatg cggttcaccc gctcgttgat ggtaaccttc
    12361 accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt
    12421 aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt cctgcacggc
    12481 ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga aactgcggta
    12541 gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct ccatcaccag
    12601 attgccaatc atcttgctcg gcacttcgcg ctcaccggtg gcgcgcagct gcgattttat
    12661 atgattgatt gccatttcga cgtcatcgga actcactgga cgtttttcca gcgcccgcag
    12721 cattccgcta cgtaatttct cttcattaaa cggttcacgc acgtcgttgc tttttacaac
    12781 acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac acaccagaca
    12841 ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa ttaccttagt
    12901 gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg ttaacagaaa
    12961 tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga taaaggatcg
    13021 atgctttcac tgcttttacc ctctaccatt aggctaattg cgacgagaaa gggacgacct
    13081 atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct tagcgcctgt
    13141 gtgcagcaaa gtgaagtgcg acagataaaa cacagcgtca gcacgctgaa ccaggagatg
    13201 acgcagctca ataaagaaac ggtcaaaatc actcagcaaa acaagctgaa tgcaaaatcc
    13261 agcagtgggg tttaccttct gcctggagca aaaacaccag caagactgga aagccagatc
    13321 ggtactttac gtatgtcgct ggtgaatatt acgcctgaca cagatggcac taccctgacg
    13381 ctacgtattc agggcgaatc caatgaccct ttacccgcat tcagcggaac cgttgaatat
    13441 gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcagaa tcaattgatt
    13501 aacgcacctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca gttaaagggt
    13561 atgtctgtgg atcagttagg ctttgtacgt atccacgaca ttcaacctgt tatgcagtaa
    13621 acgtattgcg gggcgatatt gtgcgtcccg caacatcttt ttccgtcatt ttgttactcc
    13681 gcttacatca cctggattga tagtaaaagt ttgcaacaag ggtgaaagtc agtacaatcc
    13741 ccgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac
    13801 gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta
    13861 gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat
    13921 gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa
    13981 cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt
    14041 tgtcggaagc tatcacaccc gtttcggacc gcagctccgc aacgatacct accttgagta
    14101 cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt
    14161 cttcggcggt aactccgatg caaaaggtat ctggaaccac ggttctccgc tgtttatgga
    14221 aatcgaaccg cgtttctcca tcgacaagct gaccaatact gaccttagct ttggtccgtt
    14281 caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggccg
    14341 ccagagcacc tggtacatgg gtctgggtac cgacatcgat actggtctgc cgatgagcct
    14401 gtccatgaac gtctatgcga aataccagtg gcagaactac ggcgcagcga acgaaaacga
    14461 gtgggacggt taccgtttca aagtgaagta ctttgtgccg attaccgatc tgtggggcgg
    14521 ccagctgagc tacatcggct tcaccaactt cgactggggt tccgacttgg gcgatgacag
    14581 cggttatgcg aacaacggta ttaaaacacg taccaataac tctattgcgt ccagccatat
    14641 tctggcgctg aattacgatc actggcacta ctctgtcgtt gcccgttact ggcataacgg
    14701 tggtcagtgg aatgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc
    14761 taccggctgg ggtggttatc tggtagtagg ttacaacttc taattgtgag aatgccggat
    14821 ttcatatccg gcatttttta tcacttcttc ttgttcatca tcgctttcaa atcagcgaat
    14881 gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta
    14941 ccatactgat ccgcttcggt gtatttcgaa tgctcatgat cgtggcaata gagacaaagt
    15001 aattcccagt tactgccatc ttccgggtta ttagtatggt cgtgatcaat gtggtgaacg
    15061 gtaagttcac gcaggttgga ataaacaaac tcgcgggaac agcgaccgca gacccacgga
    15121 tagattttta atgctttttc gcgatagccg ctttctaacc gcgcatagtt ttttgggatg
    15181 atagccatga aaaagttacc tggatgaaat gtagggttac gcgcagtttc ccaagatgcg
    15241 gggattttcg caatcacctt acatcaaaaa aaatcccgat cttctgaccg ggattttcaa
    15301 catcagctga aacttgatta aggcagaatt gacggctgat ccgccccttc tttttccact
    15361 ttctgctgca acatgtgttc gcgcttcata cccagtttca gagccagcgc agatgccaca
    15421 tagatggaag atgcagtacc gatggaaaca ccgataagca tggtcagcga gaagccttcc
    15481 agtaccggac caccgaagag gtacagcatc aggataacca tcaaggtagt accggatgtg
    15541 atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa agatttcgta aggcgtaccg
    15601 cgacggatct tgcggaagtt ttcacgaata cggtccgata ccacgatact gtcgttaagc
    15661 gagtaaccga taaccgacat caacgatgcc acaatggtca ggtcaatctc gatatggaat
    15721 aacgacaaaa tacccagcgt aatgataacg tcgtgcgcca gcgcaataac aacccctgcc
    15781 gccagtcgcc actcaaagcg gaaacctacg tacacgagga tagacagcag cgccgccatc
    15841 aacgccatcg caccggtttg cgcaaggtct gcccccacgc tcggaccgac gaactcaata
    15901 cgcttcactg ccgcattctg attggtggat tcgttaatca ccttcagaac ctggctgccc
    15961 aacacctgac cgccggtttc gccttcagca ggcggcatac ggaccatgat gtcgtggctg
    16021 ctaccaaagt tttgcagcat cggctcttca aaaccggctt tttgcaatgc atcacgcatt
    16081 acgtcaattt cagccggttt ttcgagcgta atttcaataa ccgtaccacc ggtaaaatcc
    16141 agcccccagt taaagccgcg cacgcccata ataacgatag cggcgattaa cagcagacca
    16201 gagatgccga aagcccagta gtcccagcgc ataaagtcat agactttacg gccgtggttt
    16261 agttgttcaa cagtatattc ctgtgccaca tcgcactcct cagattgaca gcttcttgac
    16321 gcgcttgccg ccatatagca ggtttacgat ggcacgcgta ccgacaatcg cggtaaacat
    16381 cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct ttaattgccc cggtacccac
    16441 tgcatacagg atgatgactt taatcagcgt ggtgatgttc gcatcgaaga tggaactgaa
    16501 tgcgccacga taaccttcat caattgcctg ttgaacagta cgtccgttgc tcaactcttc
    16561 tttaatacgt tcgttgatca gtacgttcgc atcgaccgct accgcaaggg ttaagacgat
    16621 acccgcgata cctggcatac tcagcgttgc gcctggcaac agcgacataa taccgacaat
    16681 caagatcaag ttggcaatca gagcactggt cgcaatcaga ccaaacttct tatagaagat
    16741 gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa gcctccagcc cctgctcaat
    16801 gttctgcata cccagggttg ggccaatggt acgttcttca acaatctgaa tcggcgcgat
    16861 caacgcaccg gcacgcagca gcagtgaaag ctgacgcgct tcgcttgggt tattgatgcc
    16921 agtaatacgg aagctgttac ccagacgaga ctggatgttg gcgatattaa tcacctcttc
    16981 ctgtttcacc agaaccgcac gaccgtttgc atctttctta ccgctgtctt tgtactccac
    17041 aaacagggtt gccatcggtt tgccgatgtt gtccttggtg aagttagaca tgatgttacc
    17101 acccgcgcta tcgagcgaga tgttaacctg cggctggttg tattcgtcct ggctggaagt
    17161 ggagtcggtg atatggtcac cggtcagaat tacgcgtttg tacagcacaa ccggctgacc
    17221 ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga ccggatgccg cagcggcctg
    17281 atcaacgttg gtgtttacca gacggaattc cagcgttgcc gtcgcaccca gaatctcttt
    17341 cgcacgcgca gtgtcctgaa tacctggcag ttcaacaacg atacggtcag caccctgacg
    17401 ctgaaccacc ggctccgcca cgccaagttg gtttacacgg ttacgcagga tattgatgtt
    17461 ctgctgcacc gcatattcac gcgcttcact cagacgagca tcgctcatta ccgcacgcag
    17521 ctggttgctg ccctggctgc taatcaccag gtccggatgg cgcttgctca gatacgcaat
    17581 ggcttcatca cgagctttag catcgcggaa agtgatgctc aggccgtagt tgttttcttt
    17641 acgaacggtg gtatacggga tgcccttttc acgcaggtcg ctgcgcaggc tatcgatatt
    17701 ttgttcctgg agtttgccaa gcgcggtgtc catatccact tccatcagga agtgaacgcc
    17761 gccacgcagg tcaaggccga gcttcatcgg ctcagcatga atagctgcca gccagcgcgg
    17821 cgttgccggg gcaaggttaa gcgccacgac gtatttgtca cccataacgc ccattaatgc
    17881 ttcacgagcg cgcagctggg tgtcagtgga gtcgaagcgc gcaagaatag cgccctcttc
    17941 cagtgccaca gacttagcag ttattttttc ttcttgtaac gttttctgga cctggatcag
    18001 cgtttgctca ctggcggcga ctccgcgcgc accagtgatc tgaacagccg gatcctcacc
    18061 aaacaggttg ggaagcgcat acagcagacc gatgacaatc accacgatca gcatgacgta
    18121 cttccacaaa ggataacggt ttaacacggc aattccctta gggaaaaatt ttaattacag
    18181 cgccttcatg gtgcctttcg gcagaacggc agctacgaag tcacgtttaa taactacttc
    18241 agtggtgtca ttcagtgcga tagcaatgta gccgttttcc gctactttgg ttacgcgacc
    18301 gaccaggcca ccgttcgtca gcacttcatc acctttagcg atggagtcca tcagcttttt
    18361 gtgttctttg gtgcgcttct gctgtggacg caggatcatg aaatagaaaa tcagaccgaa
    18421 taccaccagc atcaaaatca aagacatcgg gctaccttgc gccggtgccc ccgttgccgc
    18481 taccgcatca gaaataaaaa agctcattaa atttccctca ttattaatat taatcaacgt
    18541 tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc agttacgaag ctctctaatt
    18601 taccctcttc aatagccttg cgtaaacccg ccatcaaacg ctggtagtaa cgaaggttat
    18661 gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg gtcgagatga tgcaagtaag
    18721 cgcgtgaata attgcgacag gtgtagcaat cacactcagg atcgagtggg ccagtatcgc
    18781 tcttatactt cgcattgcgg attttcacca cgccatcggt cacgaacaaa tgaccatttc
    18841 gggcgttgcg ggttggcatt acgcagtcaa acatatcgat accgcgacgt acgccctcaa
    18901 ccaggtcttc tggtttacca acgcccatca ggtaacgcgg tttgtctgcc ggaatttgcg
    18961 ggcatacatg ctccagaatg cggtgcatat ctgctttcgg ctcacccaca gccagaccgc
    19021 cgacagcgta gccatcaaaa ccgatatcta ccagaccttt aacagaaata tcacgtaaat
    19081 cttcgtaaac actgccctga atgataccaa acagcgcatt tttgtttccg agactgtcaa
    19141 aacgctcacg gctacgcttc gcccaacgca gagacatctc catggagcgt tttgcgtaat
    19201 cccagtcagc aggatacggc gtacactcat caaagatcat gacgatatcc gaaccaagat
    19261 cgtactgaat ctccatcgac ttttccgggt cgaggaaaat cgggtcgccg ttgatcgggt
    19321 tgcggaagtg aacgccctgt tcggtgattt tacgaatatc accaaggctg aagacctgga
    19381 agccgccgga gtcggtaagg attggtcctt tccactgcat aaaatcgtgc agatcgccgt
    19441 gcagtttcat gatttcctgg cccgggcgca gccacaggtg gaaggtgttg ccgaggataa
    19501 tttgcgcgcc agtggcttca acttcttccg gcgtcatccc ttttacggtg ccgtaagtgc
    19561 caacaggcat aaaacaaggc gtttccacta cgccacgatc aaagaccagg cggccacggc
    19621 gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt ttctccacta cgtcagaaaa
    19681 acagtccaac gtttaaacca gcgccgcgga attactcccc aacgcgctca ttaattgcct
    19741 gcggattgta cgtgataaac atcgcatcac cgtaactaaa aaagcgatat ttctcttcta
    19801 ccgccgcttt ataggcattc atggtgtgtt gataaccagc aaatgccgaa accagcataa
    19861 tcagcgtcga ttccggaagg tggaagttcg tcaccagcgc atcgaccact ttgtactgga
    19921 agcccggata gataaagatt tgggtatcgt caaagaacgg ttcaatgaga tcgtttttcg
    19981 ctgcctgagc cgcactttcc agcgaacgta ctgaggtggt accaaccgca atcacccggt
    20041 taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc ctgcggcact tcagcgtatt
    20101 ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac
    20161 caacgtgcaa cgtcacaaac gccatctcca cgcctttggc gcgtaatttt tccagcaaag
    20221 gttcgtcaaa gtgcagtccg gcggtcggtg ctgctaccgc gccgggtttt tcgctataaa
    20281 ccgtttgata aagttcgcgg tctgcatctt cgtccggacg gtcgatgtac ggcggcagcg
    20341 gcatatggcc gatgctgttg agaatatcca gcaccgaacg ttgatcatta aattcgactt
    20401 caaacagtgc gccgtggcgc gcggtcattg ttgcgttaat actttcgtca tcgcccagca
    20461 gcagttctgc gccaggtttt ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt
    20521 cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc gctggcttta cgcccaaaca
    20581 gacgcgccgg gatcacgcgg gtattattaa aaaccagaag atcgccgggg ttgagcttat
    20641 cgagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta
    20701 aacgacagct actgcgttca ggcatgggat agtgggcaat cagggattcg ggcaattcaa
    20761 aagagaaatc ggtaacgcgc atgacactga ctcagactaa aataagaggc gggtagtcta
    20821 gtgccggggc gctctccctg caacctttac ccccctttgg atagaatata atcgatgaat
    20881 tttttagctc acctgcattt agcccatctc gcggaaagct cgctttccgg caatttactg
    20941 gctgatttcg tacgcggaaa tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt
    21001 catatgcatc ggcgaatcga cgtattaacc gacaatctgc cagaagtccg cgaagcgcgg
    21061 gagtggtttc gtaatgaaac gcgccgtgtc gcgcctatta cgctggatgt gatgtgggat
    21121 cactttctgt cccgccactg gtcgcagctg tcgcccgatt ttccgctaca ggaatttacc
    21181 tgttatgccc gggagcaagt gatgactatt cttccagact caccgccacg ctttatcaat
    21241 ctgaacaact acttatggtc agagcggtgg ttggtgcgct atcgcgatat ggatttcatc
    21301 cagagcgtgt taaacggcat ggcaagccgt cgcccacgtc tggatgccct gcgtgactcc
    21361 tggtacgatt tagacgccca ttacgatgct ctcgaaaccc gcttctggca gttttatccg
    21421 cggatgatgg agcaggcgtc acgcaaggcg ttataagacg ttaattcatc cacaccgtag
    21481 ccgaaatggc aggcaaagcc agaatcccgt ccgtcagttg cccgtgacct tctttgcgtt
    21541 gccattgcgc gacattaagc aacggcgacg cgggtagcac cacttcacag gcttcgccac
    21601 ggttgattgc caccagtaca cgctgctgat tcagcacgcg gacaaatacc accacgttat
    21661 cttccgcata cagcacctga cagccgccac gacgcaacgc ctgacttttc ttacgcagcg
    21721 caatcattcg ctggtacagc gcgaataacg ccgaatcctg cttttccacc tgccagggga
    21781 acggcttacg gcaaaacgga tcgtttttgc catccagtcc cacttcatca ccgtaataaa
    21841 tgcacggtac accaggccag gtgaacagcc agaccaccgc caggggcagg cgcgcaacat
    21901 cccgaccgag cagcgtttta aatcgcgcag tatcgtggct gtcgagctga ttaaacatac
    21961 gtaattgttg ttgatgagaa agccctgcgc ggtaattatc catccaggcc atacaggttt
    22021 gggcgtcaat ttgctgcgga tcgtaagaga tatcggtgtt ggcaagaaat ccccacaacg
    22081 ggaatgtgaa gccgcgatag ttcatggcgg catcttccac atcggcctgt aaccattgcc
    22141 gtgcatcgcc aaaatgttcg ccgacaatat acgcatccgg ctgggtttct ttcgccgctt
    22201 cggtgatccc ggcaacgtgc tgcaaattat tgcgcacccc acccgcttcc cccagcatat
    22261 gcaccacatc cagccgccag ccatccatat tccacggcgc tttcagccag tggcggacaa
    22321 tactgtcttc cccgcgataa atttcattca ccagactttc cgactgataa tccagcttcg
    22381 gcaagctggc atagcccagc cagtcgagcg ccgtgccatc atcactaaac gagtaccagt
    22441 cacgccaggg tgattcgggg ttgtgacaag ctccccccgt gccacgattg tgcctgtcaa
    22501 accaggcatg ggaatcgcca ctgtggttaa acacgccgtc cagcaccagt cgcattccca
    22561 gctgctgcgt attgtgtcgc aaacgcagca acgccccatc accgccaaac tgcggatcga
    22621 catggcgata atcctcggta tcgtatttat gcacgctggg agctttaaac accggattga
    22681 gatacagcgc cgtcacgcca agttttttca gatacggcag tttttcactt atcccgtcca
    22741 gatcgccgcc atagaacgtt gatccgccag cctgcgccgt gaccggttca tcccagtcac
    22801 gcaatatgat ctcttgtccg gctgcgtgat ggtaatagac atgctcctgt tcagcttcac
    22861 gaggaagatt acgggcgaaa cgatcaggga agatctgata aaaaatctga tccgcagccc
    22921 attgtgggcc gatatccggt acatcaatgg caaactgctc cagtcgtgct ggcggcattc
    22981 ggctgaagcc ttgcggtgta aaccaacgct ggcgatcgtg ccacagcaat ttgaaactat
    23041 aacgccgccg aggttgtccg caggagagct cgatcgccgc ccgccatgcg gtgacgacag
    23101 gctgcggctg actgcgctgc ttatgcatcg gtactgacat ttcttcgtta tcgtgttctg
    23161 tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag ccacagtgta atgagcagtt
    23221 gatctttgct ttgtttaaca aatgggggca ccggcagatg ccatgcattt aacatcataa
    23281 ttcccctgtg atgaaaatct gatcaccttg ccatagccta atgcaggatc actcatcatc
    23341 tgaaggatat ttgggggagg aggacggggg cggataactt tcggaatagc cggatcagga
    23401 tttcctcctg aaccggctta cagaacgcaa ggattactgg ttttcagcca gctgacgatc
    23461 gtggcggcgc ttaaacatcc agccaatcaa cagcacaaca atccacgcga aaccgacata
    23521 cagcgagata cgcgtatccg ggtgataacc aatcaacccg ataataaaga gcagaaaaat
    23581 caaaccgccg atggtggttg ctaccccacc cggtacttta aatttcagcg ccttaacttc
    23641 ttctggcggt aaacggcgac ggaaggcgat ttgcgacagc aggatcataa tccacaccca
    23701 caccgtggcg aaggttgcca gcgaagcgat caccaggaag acgttttccg gcatgatgta
    23761 gttcagatac accgcaaaca gcagcgcggt agtcatcacc agcaccgtaa cccacggaat
    23821 accgcgacgc gaggttttgc tgaaaatttt tggcgcgctg ccctgctctg ccataccgtg
    23881 gagcatacgg cctacaccaa atacgtcact gttaattgcc gacagcgaag cggtcagcac
    23941 aacaaagtta agaatgctgg cggcaaaggt aatgcccata tgctggaacg tcagcacaaa
    24001 cgggctaccg gcagtgccaa cctgattcca cgggtagata gacataatga cgaacagcgt
    24061 accgacgtag aacaccagaa tacgcatcgg cacggagtta atcgcacgcg gaatcgattt
    24121 ctcaggatct ttcgcttcac cggcggtaat cccgataatt tcgatcccac cgtaagcaaa
    24181 catcaccatt tgcaacgaca tcaccatacc aagccagccg ttactgaaga agccgccgtt
    24241 gctccacagg ttatgaatac cggtcggttg cccgccgttg ccaatccccc agatgatgat
    24301 gccgaaaccg gcgacaatca tgatgatgat agtggcgact ttaaagaacg agaaccagaa
    24361 ctccagctca ccgaatacct tcacgctcat caggtttacg gcgcaaatga tcagcaccac
    24421 actcagtacc caaatccagt gcggcaccgt cgggaaccag acacccatat agataccaaa
    24481 agcggtcaca tcggcgatgg cgacaataag gatttcaaag cagtaggtcc agccggtaat
    24541 gtaacctgcc agcgggccga ggttttcctg cgcataacgc gagaaagagc tggctgccgg
    24601 gttatgtacc gacatttccc ccagcgcacg cataatgata tacgccgcga taccaccgat
    24661 aatataggcc aacaacacgc ttggaccggc cattttgatg gcatctgccg aaccgtaaaa
    24721 cagcccggtg ccaattgctg aacccagtgc cataaagcgt atgtgtcggg tacttagccc
    24781 acgctttagc ttgttcttac tttccatcaa ttcctaaccc atcaacacaa taaaaaacca
    24841 cggggcatcg cagccccgtg gttaaaacaa atgttcagtg atttagtgag cgctggaggt
    24901 cacctgacga cctgccgcac gatcccagat aatggccaga accaccatca ccactgttgg
    24961 cattaaccac gccagacctt gttcggccag cggtaaacgc tgcgcccagg acggtaagat
    25021 atcgctgaat gcagatgttt tgatcccgtc gagaatacca aaaagcaggc tgataaacat
    25081 cggcggagca atcacgcggg acgaattatg ccaccatgag cgtgtaaaac ttaatacaac
    25141 cagtgcgata cacggcggat aaatggcggt cagcaccggt acagagatct gaatcagctg
    25201 gctcaagccg aggttagaca ccaccatcga gaagccgccg aggataaaca ccagcgtacg
    25261 ataagagagc ggtacgtact gggcgaagaa ttctgcacaa gcacaggtca ggccaaccgc
    25321 cgtgaccagg caggcgatga agattaacgc cgccagcagg aagctaccgc cgccgccaaa
    25381 ggtatgctga acgtaagcat gcaggatcgc cgcaccgttt gcagactgat cgaccagcga
    25441 cgcgctgtct gaacccagac ggaacagcgc caggtacagc agagtcagac caacacccgc
    25501 catcaggcca gcccagacgg tataacgggt cagcagacgc gcttcggtaa cgccacgaga
    25561 acgcgccgcg ttaacaataa cgataccaaa caccattgcg cccagcgtat ccatggtcag
    25621 atagccgttg acgaagccgt tagaaaacgc agcgttttga taagcctcag tcgccgtgct
    25681 gatagaaccc gccggccaga taattgcggc aacagacagg atgaccagcg cgataatttt
    25741 cagcggcgca aggaagttgc ccacggtatc cagcagcttg cccggataga gcgaaaccag
    25801 aataacgata gcgaaataga ccaggctgta aataaacagc ggcagcgcgg aatcacccgt
    25861 cagcggcgca atgcccactt caaaagaaac ggtagctgta cgcggcgtag cgaaaagcgg
    25921 ccccaccgcc aggtaacaaa ctgttgccag cagtacgcca gcgactttac caattggcgt
    25981 gctgagactg tcaacaccgc cgccaacttt tgccagtgcc actaccgtta ataccggtag
    26041 gccaacggca gtaatgagga agccgaatgc cgcagtccag acgtgttcgc ctgcctgcaa
    26101 gccgaccatt ggagggaaaa taatgttacc tgcgccgacg aacaacgcaa atgtcataaa
    26161 gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg
    26221 atgtggtgtt gtaacgttaa atttttgtgc caatccctgg ggatgataca gcggtatatt
    26281 taaacaattt tagcgggaaa tactcccaac caggcgcggc gaagaatagt ttttcgattt
    26341 accgcgtaaa tgcagtcagt atgacagcac ctgaagcgca atttaaacgc ttataacgtt
    26401 ttaaagcaag aagggatcta aaaaccagag gtatatagcg ataagaaatc accaaccagt
    26461 ataaaacact atttcaatga aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc
    26521 attgtaaaat ctaacgcccg tatttcgact gcaaagaaaa taagccagca tcggctggct
    26581 tatggcaata aaagatgaca aaggcgaatt aatcgctgtt tttggcaatt aaacgttccg
    26641 ggataacaaa actgaaacgt gtaccttttc ctactgtact ctcaatattc aggcgacttt
    26701 cgtgatgatt caccgcatgt ttcacgatcg ctaaccctaa tccgctacca ccggtttgcc
    26761 gggaacgcgc tttatcaacg cgataaaaac gctcggtcag gcgcggaata tgctccggtg
    26821 caatgcccgg tccgttatct tcaacactaa attcggcacc gtgcggcact cgctgccagc
    26881 gtacggtgat atgcgtgcct tccggcgtat gattcacggc gttatagacc aggttcgaaa
    26941 tcgcactgcg tagctgatct tcgttgcctg acaccttaag gccgttatct atctcaaagg
    27001 taaatgtctg ttttttctga ctcagagtct gagcctcgcg ctcaacgacg cgcagcatca
    27061 tcggcacatc aaccttttca ttgagcaaat gcgtcggtgc ggcttctatt ttcgacagcg
    27121 tcagcaattg cttcaccaac ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg
    27181 ctttttcacg taccgcgcct tccagcggtt gctcatccat catctccagg taaccctgta
    27241 acacggtcaa tggcgtacgt aactcatggc tcacattggc aaaaaagtta cgccgcgccc
    27301 cttccagttg atgcatttgc gtgacatctc gcgccaccat cagcaactgt ttgtgggtat
    27361 aaggcatgac gcgaatctcc agatgccgcc cggtgttgag caccagattg agcgggcgag
    27421 agaaatcacg cgttttcaga tattgcgtaa actccgggta acgcagcaag ttaaggatgt
    27481 tctgcccgtt atcttccggc cagcgcaagc caagaatctg ttgcgccaga ccgttgcacc
    27541 agaaaatacc gccctcttcc gtggtcagca ccaccgcgtc gggcagcgac tccgcgccgc
    27601 tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg ttttttattt cgcagctgca
    27661 tctggtgtaa gccgtatagt agcggttccc agctaccacg ccccggtggc ggggtcatac
    27721 tacgatccac ccacagccac catgaaaggc gcaataaatt ccagaaatgc cagataagca
    27781 gtcctgttac cgatgccagc aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga
    27841 aagccgggag gcagcaaagt agcagctcca gcaccagcct tttccacgac agccgttcca
    27901 gcacgtaaga tactccagtt aagaaatcat aagccctgct ctacgtccga tgagcaaggc
    27961 cttaaaagcg ggttgaaaaa cgataccctg taccgcgcac ggtctgcacc atgcggtcat
    28021 gcccgccggg ctccagtgct ttacgcaggc gacgaatgtg gacatcgacc gtacgatctt
    28081 ccacatacac gttggttccc cagacgtggt ttaacagctg ctcgcggctg tacacgcgct
    28141 caggatgcgt cataaaaaag tgcagcagtt taaattctgt cggccccatc tccagcggtt
    28201 cttcgcccgc catcacccgg tgagatgtcg ggtccagact tagcccctgc atctcgatca
    28261 cctcttccac cgccattggc gaaatacggc gcattaccgc tttgattcgc gccaccagct
    28321 ccttcggaga aaacggcttg gtgatatagt catccgcacc ggtttcaagg ccgcgcacgc
    28381 gatcttcttc ttcccctctg gcggtcaaca tcaccactgg aatatcccgg gtcatcgact
    28441 cgcgcttgag gtgtttgatg aactggatac cggagccgcc aggtaacatc cagtcgagga
    28501 gaattaaatc cggccagggt tcattcagtt gattcacagc actgtcataa tcttccgctt
    28561 cgaccggctg aaagccattt tgttcgagca cgaagcagac catttcgcga attggagctt
    28621 catcttctac gaccagaata cgtctcgcca tgatttgccc tgttgtaata aataggttgc
    28681 gatcattaat gcgacgtcat tatgcgtcag atttatgaca gatttatgaa aagctcgtcg
    28741 cacatccttt caggttattg atttccgtgg cacagaaaaa agcaaatggc acatctgttt
    28801 gggtataatc gcgcccatgc tttttcgcca gggaaccgtt atgcgcatcc ttcacacctc
    28861 agactggcat ctcggccaga acttctacag taaaagccgc gaagctgaac atcaggcttt
    28921 tcttgactgg ctgctggaga cagcacaagc ccatcaggtg gatgcgatta ttgttgccgg
    28981 tgatgttttc gataccggct cgccgcccag ttacgcccgc acgttataca accgttttgt
    29041 tgtcaattta cagcaaactg gctgtcatct ggtggtactg gcaggaaacc atgactcggt
    29101 cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc aatactaccg tggtcgccag
    29161 cgccggacat gcgccgcaaa tattgccccg tcgcgacggg acgccaggcg cagtgctgtg
    29221 tcccatacct tttttacgcc cgcgtgacat tattaccagc caggcggggc ttaacggtat
    29281 tgaaaaacag cagcatttgc tggcggctat tactgattat tatcaacaac attatgccga
    29341 tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc accactggac atttaacgac
    29401 cgtgggtgcc agtaaaagtg acgccgtgcg tgacatttat attggcacgc tggacgcgtt
    29461 tccggcacaa aactttccac cagccgacta catcgcgctc gggcatattc accgcgcgca
    29521 gattattggc ggcatggaac atgttcgcta ttgcggttcc cccataccac tgagttttga
    29581 tgaatgcggt aagagtaaat atgtccatct ggtgacattt tcaaacggca aattagagag
    29641 cgtggaaaac ctgaacgtac cggtaacgca acccatggcg gtgctgaaag gcgatctggc
    29701 gtcgattacc gcacagctgg aacagtggcg cgatgtatca caggagccgc ctgtctggct
    29761 ggatatcgaa atcactactg atgagtatct gcatgatatt cagcgcaaaa tccaggcatt
    29821 aaccgaatca ttgcctgtcg aagtattgct ggtacgccgg agtcgtgaac agcgcgagcg
    29881 tgtgttagcc agccaacagc gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa
    29941 tcgccgtctg gcactggaag aactggatga atcacagcag caacgtctgc agcatctttt
    30001 cgccacgacg ttgcatagcc tcgccggaga acacgaagca tgaaaattct cagcctgcgc
    30061 ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg atttcacccg cgagccgttc
    30121 gccagcaacg ggctgtttgc tattaccggc ccaaccggtg cggggaaaac caccctgctg
    30181 gacgccattt gtctggcgct gtatcacgaa accccgcgcc tctctaacgt ttcacaatcg
    30241 caaaatgatc tcatgacccg cgataccgcc gaatgtctgg cggaagtgga gtttgaagtc
    30301 aaaggtgaag cgtatcgcgc gttctggagc cagaatcggg cgcgtaacca gccggacggt
    30361 aatttgcagg tgccacgcgt agagctggcg cgctgtgcag acggcaaaat tctcgccgac
    30421 aaagtgaaag ataagctgga actgacggcg acgttaaccg gactggatta cgggcgcttc
    30481 acccgttcga tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa
    30541 gaacgcgcag aattgctcga ggagttaacc ggcactgaaa tctacgggca aatctcggcg
    30601 atggtttttg agcagcacaa atcggcccgc acagagctgg agaagctgca agcgcaggcc
    30661 agcggcgtcg cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta
    30721 cttactgacg aagaaaaaca gttaattacc gcgcagcagc aagaacaaca atcgctaaac
    30781 tggttaacgc gtctggacga attgcagcaa gaagccagcc gccgtcaaca ggccttgcaa
    30841 caggcattag ccgaagaaga aaaagcgcaa cctcaactgg cggcgctcag tctggcacaa
    30901 ccggcacgaa atcttcgtcc gcactgggaa cgcatcgcag aacacagcac ggcgctggcg
    30961 catactcgcc agcagattga agaagtaaat actcgcttac agagcacaat ggcgcttcgt
    31021 gcgagcattc gccaccacgc ggagaagcag tcagcagaat tacagcagca gcaacaaagc
    31081 ctgaatgcct ggttacagga acacgaccgc ttccgtcagt ggaacaacga actggcgggt
    31141 tggcgtgcac agttctccca acaaaccagc gatcgcgagc atctgcggca atggcagcaa
    31201 cagttaactc atgctgagca aaaacttaat gcgcttgcgg cgatcacgtt gacgttaacc
    31261 gccgatgaag ttgctagcgc cctggcgcaa catgctgagc aacgcccact gcgtcagcgc
    31321 ctggtcgcac tgcatgggca gattgttccc caacaaaaac gtctggcgca gttacaggtc
    31381 gctatccaga atgtcactct ggaacagacg caacgtaatg ccgcacttaa cgaaatgcgc
    31441 catcgttata aagaaaaaac gcaacaactt gccgatgtga aaaccatttg cgagcaggaa
    31501 gcgcgcatta aaacgctgga agcccagcgc gcacagttac aggcgggtca gccttgtcca
    31561 ctttgtggtt ccagcagcca cccggcggtc gaggcgtatc aggcgctgga accgggcgtt
    31621 aatcaggccc ggctattaac gctggaaaaa gaagtgaaaa agctcggcga agaaggtgcg
    31681 gcgctacgtg ggcaactgga tgcattaacg aagcagcttc agcgcgatga aaacgaagcg
    31741 caaagcctcc gacaagatga gcaagcactt actcaacaat ggcaagccgt cacggccagc
    31801 ctcaatatca ccctgcagcc acaggacgat attcaaccgt ggctagatgc acaagatgag
    31861 cacgaacgcc agctgcggtt actcagccaa cggcatgaat tacaagggca gattgccgcg
    31921 cataaccagc aaattatcca gtatcaacag caaattgaac aacgccagca gcaactctta
    31981 acggcattgg cgagttatgc actgacattg ccacaggaag atgaagaaga gagctggttg
    32041 gcgacgcgtc agcaagaagc gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg
    32101 caaaaccgta tgcagcagct gacgccgatt ctggaaacgt tgccgcaaag tgatgaactc
    32161 ccgcacagcg aagaaactgt ggcgctggaa aactggcggc aggttcatga acaatgtctc
    32221 gcattacaca gccagcagca gaccttacag caacaggatg ttctggcggc gcaaagtctg
    32281 caaaaagccc aggcgcagtt tgacaccgcg ctacaggcca gtgtctttga cgatcaacag
    32341 gcgttccttg cggcgctaat ggatgaacaa acactaacgc aactggaaca gctcaagcag
    32401 aatctggaaa accagcgccg tcaggcgcaa acgctggtca ctcagacagc agaagcgctg
    32461 gcactgcatc agcaacaccg acctgacggc ctggatctca gtgtgacggt ggagcagatt
    32521 cagcaagagt tagcgcaaac tcagcaaaag ttgcgtgaaa ataccaccag tcagggcgag
    32581 attcgccagc agctgaagca ggatgcagac aaccgtcagc aacaacaaac cttaatgcag
    32641 caaattgctc aaatgacgca gcaggttgag gactggggat atctgaattc gctaataggt
    32701 tccaaagagg gcgataaatt ccgcaagttt gcccaggggc tgacgctgga taatttagtc
    32761 catctcgcta atcagcaact tacccggctg cacgggcgct atctgttaca gcgcaaagcc
    32821 agcgaggcgc tggaagtcga ggttgttgat acctggcagg cagatgcggt acgcgatacc
    32881 cgtacccttt ccggcggcga aagtttcctc gtcagtctgg cgctggcgct ggcgctttcg
    32941 gatctggtca gccataaaac gcgtattgac tcgctgttcc ttgatgaagg ttttggcacg
    33001 ctggatagcg aaacgctgga taccgccctt gatgcgctgg atgccctgaa tgccagtggc
    33061 aaaaccatcg gtgtgattag ccacgttgaa gcgatgaaag agcgtattcc ggtgcagatc
    33121 aaagtgaaga agatcaatgg actgggctac agcaaactgg aaagtgcgtt tgcagtgaaa
    33181 taactattca gcaggataat gaatacagag gggcgaatta tctcttggcc tcgctggtcg
    33241 ttatcctgca agctatcact ttattggcta cggtgattgg tagccgttct ggtggttgtg
    33301 atggtggtat gaaaaaagtt attttatctt tggctctggg cacgtttggt ttggggatgg
    33361 ccgaatttgg cattatgggc gtgctcacgg agctggcgca taacgtagga atttcgattc
    33421 ctgccgccgg gcatatgatc tcgtattatg cgctgggggt ggtggtcggt gcgccaatca
    33481 tcgcgctttt ttccagtcgc tactcactca aacatatctt attgtttctg gtggcgttgt
    33541 gcgtcattgg caacgccatg ttcacgctct cttcgtctta cctgatgctc gccattggtc
    33601 ggctggtatc cggctttccg catggcgcat tttttggcgt cggagcgatc gtgttatcaa
    33661 aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc gggatgacag
    33721 tcgccaattt gctgggcatt ccactgggaa cgtatttaag tcaggaattt agctggcgct
    33781 acaccttttt gttgatcgct gtttttaata ttgcggtgat ggcatcggtc tatttttggg
    33841 tgccggatat tcgcgacgag gcgaaaggaa aactacgcga acaatttcac tttttacgca
    33901 gccctgcccc gtggttaatt ttcgccgcta cgatgtttgg caacgcaggt gtgtttgcct
    33961 ggttcagcta cgtaaagcca tacatgatgt ttatttccgg tttttcggaa acggcgatga
    34021 cctttattat gatgttagtt gggctaggga tggtgctggg gaatatgcta agtggcagga
    34081 tttcaggacg ttattcacca ctgcgcattg cagcagtgac tgactttatc attgtactgg
    34141 cactgctgat gctctttttc ttcggcggta tgaaaacaac gtcgcttatt tttgctttta
    34201 tttgttgcgc gggattattt gccctatcag cacctctaca aatattatta ctgcaaaatg
    34261 ccaaaggcgg agagttatta ggtgccgcag gtgggcaaat agcgtttaac ctcggtagcg
    34321 ccgtcggcgc atattgcggt ggtatgatgc tgacgctggg gctggcatat aattacgtgg
    34381 cgctgcccgc cgccctgctt tcgtttgctg cgatgtcgtc gttgctgctg tatggtcgct
    34441 ataagcgcca gcaagcggcg gatagttcgg tgctggcgaa accactgggg tagatagcag
    34501 tcgcagtgcg catcgcttgt tgccggatgc ggcgtgaacg ccttatccgg cctacgggac
    34561 tgttcatttt gtaggcctga taagacacgc cagtgtcgca tcaggcatcg gagcaccggg
    34621 gggttgccgc cactgtgcgc gttatttgtt gccggatgcg gcgcgagcgc cttatccggc
    34681 ctaccggacg gctcactttg taggcctgat aagacgcggc aagcgtcgca tcaggcatga
    34741 tacgccaatt gcctggtttt tttactcttg tggccataac catgcagcgc cgcgtacgcc
    34801 gctcgaatca ccatgtttcg ccttgcgtac cggcgtttca cattctccgc cgaagacaaa
    34861 ttgtttaatc aactgtggaa ccgtttgata taaacggtct acattgctca ttccgccgcc
    34921 caggacaatc acatccggat cgagaatatt cacgacatgt gccagcgatt ttgccagccg
    34981 cagctcgtag cgacgcaatg ccagttccgc taccggatcg ctttcttcaa ccaggcggat
    35041 aatttcactg cctttcagcg catgtccgct caaacgacga taatccgtcg cgaatcccgt
    35101 gcctgaaata aaggtttcaa tacaaccttg tttaccgcaa taacaaggga cttcctcgcg
    35161 ataacgcagt tcgtcttcgt ccatccacgg tagcggattg tgtccccact cgcctgccgt
    35221 gccattgccg ccgatatgcg cccgcccatt gaatgccacg cccgcgccgc atcccgtgcc
    35281 gataatcacg gcaaataccg tctgcgctcc cgctgccgcg ccatctaccg cttctgaaac
    35341 cgccagacag ttggcgtcat ttgccagccg cacttcccgc tgcaaccgcg cgcttaagtc
    35401 tttatcgaat ggctgaccgt tgagccaggt tgaattggca ttcttcacca cgccggtgta
    35461 gggcgaaatc gaaccaggaa tgcccatacc taccgttccg cgctgccctg ttgcctgctc
    35521 cgccatatca accaacgtgg cgatcgtttc aatagtctgc cggtaatcat cacgcggcgt
    35581 gggcagacga tggcggtaca actgctcccc tgcatcgccc aatgcaatca cttcagtttt
    35641 ggtgccgcct aaatcgatac ctatacgcac ggtactctcc ttattttttt caatatcaat
    35701 agcgtagaga cagacaaccg gattggcaat gcaaggccgc cgacaattcg ttatcatgcc
    35761 cgctaaattt aacgacaagg ccgtggaaat tatcatgctg tggttcaaaa atttaatggt
    35821 ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag atggaaaaac agctagcctc
    35881 gatggcattt accccatgcg gcagccagga catggcgaag atgggctggg tacctccgat
    35941 gggatcgcac agcgatgcgt taacgcacgt tgccaatggt caaattgtta tctgtgcgcg
    36001 caaagaagaa aaaatcctcc cgtccccggt gattaaacag gcgctggaag cgaaaatcgc
    36061 caaactggaa gcggaacagg cgcgtaagct gaagaaaacc gaaaaagatt cgctgaaaga
    36121 cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt tttagccaga caatgatgtg
    36181 gatcgacacg gttaacggtt tgattatggt ggactgcgcc agcgccaaaa aagcggaaga
    36241 tacgctggca ctgctgcgta aaagcctggg gtcgttaccg gttgtaccat tgagcatgga
    36301 aaacccgatt gaactgacgc tgaccgaatg ggtacgttcc ggcagcacgg cacagggctt
    36361 ccagctgctt gatgaagccg agctgaaatc gttgctggaa gatggcggcg tgatccgcgc
    36421 gaagaaacaa gatctgacca gcgaagagat caccaaccac attgaagccg gaaaagtggt
    36481 gactaaactg gcgcttgact ggcagcagcg cattcagttt gtgatgtgcg acgatggttc
    36541 gctcaagcgt ctgaagttct gcgacgagct gcgcgatcaa aacgaagata tcgaccgtga
    36601 agatttcgcc cagcgctttg atgccgattt catcctgatg actggtgaac tggcagcgtt
    36661 aattcaaaac ctgattgaag gattaggtgg cgaagcacaa cgttaattgc tgatttacct
    36721 ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt acgaagccac accaggaata
    36781 taatcgttag ctacagcggg caataattct tagcgcagca atattatgcg ttttatgctg
    36841 taacttgctc caggggcgtt gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg
    36901 cgaatttttc gcgttgcccc gtttttcact aaatcacgta acagcgaaaa atcgggttgt
    36961 tcgacaaata agagtaatga tggctcctct tttaaccacg tagttttcac atccgcagcc
    37021 agctgtgaat tattaatagc cagcgaccgc tcctgaggct cagtcaatgt cgttaacaac
    37081 accggagtat gtgcgttgtg caatgccgtt tggcgcaaat gatcattgcg actttgcagc
    37141 aatgcacacc accattcatc atctgattta tcttttaacg tcgccagttc cgcaacaata
    37201 tctggagatc ttcgctgata tttatatcaa ccttgatgaa ttatgaatat atcgaaaatc
    37261 gaaaaaatcc cagcaaagga aatattatta tgggttaaag agaggacatt tcagaaaaat
    37321 gccctcccgt tcaaggggaa ggcgaggaat tacagatagc ggcacagata agaggtgggt
    37381 tcggcaactt gcagatgaaa cttgctgtga ccgggaacat taaacaccga accggcttca
    37441 tacacttgcc agtcggtcgc gtcaggcagt aacacattca gcgcgccact gattaccgtc
    37501 atctcttcag gctcagcggt gctgaaggtg tattcacctt caaccataac acccacgctg
    37561 gcgcgaccag tgctgctgct ggaaaagccg atcgatttca ctttgccgga aaagtactca
    37621 ttactttgaa gcataaactg gccctgtctg gtagttgaac atctacttca ctataggggc
    37681 cagaggcgct ggctgtcacg caaaattaca tcagtaattc cgcagccagc cgtgcaatca
    37741 atacgttaga gagcaagaca ggaacatcga gctgtttttg cagtaaatcg cgatgacgct
    37801 ggttaaatcc caaacaatcc agcatgatga catctgcacc ttttgccagt aattctttcc
    37861 cggcatcaat gattttttgt tccgaatcat gaatggggtt acccaatgaa aatactggcg
    37921 gtttctgcaa aatttgccat ttttgcgcct gaacggtcag caactcctca accggtacga
    37981 taacccccac ctgatgatct tcaacaatag aggaaaccag cggtggcaat atccgcgacg
    38041 gctcaagaaa gatcgtatta cgcgcggtca tactactaat gtttgctgta ctcattaata
    38101 taatgacgtc gtaaccctga ttatcgagca cttcaaccac accttgcagg tctcgctcca
    38161 ctttgcgacg cgaaacatgg gccagatggt tgtcatttaa taatgtgaga attgtgtctt
    38221 cgcctgcttc tggcgcgtac tccgccatca cttcttcgcg gcttaacttc cccagcaggc
    38281 tatgatggga aatattatct tcgtcaatgt attctgtcag gagcggcaaa acttcctgca
    38341 taggtacaat gccgatagtc aggatcgcca acgacgcact cattgttctt ccaccttact
    38401 tttctttcgt cgttaattac cgggcaagtg ttaagcacca ctgctgacac ttacttgtgc
    38461 tacaaatgac aaaaagcgta gcagcagacc tgcactgcat aatgtaaaga ttccaaatga
    38521 ttccagtaat ggattgttat tgtttaatat tctaattatt tgaaaaacat gaattgtgaa
    38581 aaatgtgacg cagatccctt gtggcggcac tcagaatccc ttccgagcag tctgatgcgg
    38641 tcactctttg tcagggtcct catagcgttt tttcgcatcc atcgcttcct gagcggtcgg
    38701 atgttcactg atcaacgagt ctggtttagg atgatcggct ctgagttggt accaggttac
    38761 cgtctgtcct ggctttcctt tatcaatcgt cactatatat gcttcacgag gataaggcgg
    38821 tttcgttggc atcgttcttc cttatttcac gggatgaacg ttaagtatag gcgctcgaaa
    38881 atcaacaatt gatcgtctgt gccagagcgc tgcgaatttc agaaatcacc tggctgggtt
    38941 cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca
    39001 gcacttcctg aacttcttcg ctcagtggtt ttcccgttaa ggttggccgt aaatcttctt
    39061 ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa taaaccacga
    39121 tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataatgccg ccgcctgtag
    39181 cgataacggt ggatggcgca gttaccgctt ccagcgccgc cgtttctctg gcgcggaatc
    39241 ccgcccactc ttccctttcg acgatctccg cgaccgtcat attgagctgt gattgcaacc
    39301 acaggtcggt atcgacaaac cgacggttaa gcgaatcggc aagggccatt ccgaccgttg
    39361 ttttaccaca gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc
    39421 aataggtcgc aatgcggcga aagccggtgt catgagaata gcgatcatac catcaaacta
    39481 gtacaatttc gattgtaaat aaaaaattcc acttaaggtg gaaatctcaa tacatccctt
    39541 actataccaa taaatattca agaatgaagt gtaaataata aattacattt agccacgact
    39601 acgttgcact tccagccacc acttctccag ctccgcagca aaagcctggc ggtcacgttg
    39661 tgaaagacta tctggtcctc cggtctggat cccactggca cgtaaggtat ccataaaatc
    39721 gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc gcggattaag
    39781 cgccgccgcg cctttctcga tggcttcagc agccaaaggt atatctgcgg tgatcaccaa
    39841 atcgcccgct tcacactgcc gaacaatttc gttatcagca acgtcgaaac ctgccgcgac
    39901 gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac
    39961 cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca
    40021 cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt
    40081 gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca
    40141 atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca
    40201 atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa
    40261 tctgggtata taccccctcc ccggataaag tcaccgccct gcatgaccag ttcggcatca
    40321 acgccgcaga atcggcgcaa gaagtagcgc aaatcgccga catcattttt gccgccgtta
    40381 aaccaggcat catgattaaa gtacttagcg aaatcacctc cagcctgaat aaagactctc
    40441 tggtcgtttc tattgctgca ggtatcacgc tcgaccagct tgcccgcgcg ctgggccatg
    40501 accggaaaat tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc ggtatgacct
    40561 ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc
    40621 gctgctttgg cgaagcggaa gtgattgctg agccgatgat ccactcggtg gtcggtgtga
    40681 gcggttcttc gccagcctac gtctttatgt ttatcgaagc gatggccgac gccgccgtgc
    40741 tgggcgggat gccacgcgcc caggcgtata aatttgccgc tcaggcggta atgggctccg
    40801 caaaaatggt gctggaaacg ggagaacatc cgggaacact gaaagatatg gtctgctcac
    40861 cgggaggcac caccattgag gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag
    40921 tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa tcctgatggc
    40981 ttttgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgccttgta
    41041 gagcgccaaa tctgccgatt tcaaccactc gcgatagtga ctcatttgtg gattcagcgg
    41101 cgcaaccccc acactaatcc gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt
    41161 tagcccttca tgcacccgta acatggcggt gatggcgctc tctgctggcg taccggacat
    41221 gattactgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gtagggtaat
    41281 ttgtaactgt cgggtaagcg ccacaatcgc ttcatcaccc acatcatggc cccaggtatc
    41341 gttgatgctc ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc gattatgccg
    41401 ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc
    41461 cgtcataccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttagt
    41521 cgccgtctgg tagctgaccc agccaaacag cagaggataa atgacaataa tgggaaggga
    41581 gagccaccat tccagcggtg cgctattgaa cgacacggtg atgcccgtca gctcgagggt
    41641 gacaaggcag gagaccacca tcaacaccag acccgcgaca aacagacggg ggccgcctgc
    41701 ccccatcaaa ttcagacaca taatcatcaa catcgcggtg gaaggcagtg cgtttacgcc
    41761 cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg
    41821 gctaagcgga tcgacggccc tgctcgctat ttgccaggct aaatgcggcc agacgaacgc
    41881 ccagccgacc aacaacagcc accaccagcc cggcgacgga tgcgaaacca gcgttgaagc
    41941 aatcggtaag aacatgccag ccaggccaac cgcgcggggt agtctgacgc gacgagcaaa
    42001 gcgcagcccg gaccgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc
    42061 cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt
    42121 ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt gagccatttt
    42181 cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcactcata
    42241 aacttattac gatcatcccc tttcagagat tgttgtgttg cttgattatt acattcgcgc
    42301 atcttttgct gctgtggcgt caaacttttt tcaccaggcg cagatttgct gttcttcagg
    42361 caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga
    42421 ttacaagagg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc gccgacggtg
    42481 gttaaaaaaa ccagaccgaa aagcaaggta accagtaatg taattttcat cgcaccatcc
    42541 ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct gtcattaagt ctggttgcta
    42601 acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc
    42661 cagggcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc
    42721 ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc
    42781 ttcggagttc ccgtaactca tcaccatcac tgcgccatct ttggtattta gcgcctgggt
    42841 gaggcccgga gctttggtat ctggcgcaac tatctggctg gcatgggcgt gatcagcggt
    42901 gactatgacc aacgtgttac catccttttt agcgaattcc agcgcccgtt gtacggcttc
    42961 atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc
    43021 gattgacgca ccttcaactt gcaggaaaaa gcctttctca tttctactca acaattcaat
    43081 ggctttgtcg gtcatctgcg ccagggtcgg tacactatca ttacgttgcg gattaggcgt
    43141 acaggtgact gcgggcttat ctatattacc gtggtacgtt gctttcggtc cttgccagcg
    43201 cactggcata ttgccgtcag caaacagtcc taataggggt ttttgctgat tcgcttccgt
    43261 caccgaattc agtgaggcag catcgctcac caactgataa ccacgcgcct gtgcctgttc
    43321 acgcagcgtt tttccctgcc attcaccggc ggttgccgtt tcagcaaagg tttttgcgcc
    43381 gccgccaagc gtaacatcgg cacgggcgtt aagcagctgt tcggtaatcg atccttttcc
    43441 gcctttttcc agagcgttac ccggacattt ttcactggtc gcactcggac cgtagcattt
    43501 gcgcgaggtc acatgcgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga
    43561 aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt
    43621 ttcgtgaata tcgacgccca gcgcgccatt ataggttttg acacctgttg accaggcggt
    43681 tgctgatgca gccgagtcgg tgacgtagtc cggtttgccg gtttttttat tcagcgcata
    43741 gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc
    43801 ttcggcataa ttgcgtgcgg cagtaatttc cgagtccccc atcccatcgc caatcagcaa
    43861 aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcgg tctgatcacc
    43921 cgttaagcgg cgagcaccgc cgggtgcagt aatatcgccc tgagctgccc ggttttccag
    43981 aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg gtaagagtgc
    44041 cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa
    44101 aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa
    44161 ctttttactg acgaggatgc ctcaataact tctttacgta atcgcgcagc agctccgtat
    44221 cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa
    44281 tcaaccgctg ttgatcattt tgcgccatat tgcgaagcat tgccgtgacg ataatctcca
    44341 aagcttctac ctgcgcgcac agttctttcg actcttcttc tttttgggca agcttaaata
    44401 acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaa
//
LOCUS       DAZFRE010000029        42001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFRE010000029 DAZFRE010000000
VERSION     DAZFRE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.029"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(51..1910)
                     /gene="gss"
                     /locus_tag="ACHKUI_001942"
     CDS             complement(51..1910)
                     /gene="gss"
                     /locus_tag="ACHKUI_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6598138.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2115..2981
                     /gene="yghU"
                     /locus_tag="ACHKUI_001943"
     CDS             2115..2981
                     /gene="yghU"
                     /locus_tag="ACHKUI_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6598139.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3051..3299)
                     /gene="hybG"
                     /locus_tag="ACHKUI_001944"
     CDS             complement(3051..3299)
                     /gene="hybG"
                     /locus_tag="ACHKUI_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6598140.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3312..3653)
                     /gene="hypA"
                     /locus_tag="ACHKUI_001945"
     CDS             complement(3312..3653)
                     /gene="hypA"
                     /locus_tag="ACHKUI_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6598141.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3646..4134)
                     /gene="hybE"
                     /locus_tag="ACHKUI_001946"
     CDS             complement(3646..4134)
                     /gene="hybE"
                     /locus_tag="ACHKUI_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6598142.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4127..4621)
                     /gene="hybD"
                     /locus_tag="ACHKUI_001947"
     CDS             complement(4127..4621)
                     /gene="hybD"
                     /locus_tag="ACHKUI_001947"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6598143.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4621..6324)
                     /gene="hybC"
                     /locus_tag="ACHKUI_001948"
     CDS             complement(4621..6324)
                     /gene="hybC"
                     /locus_tag="ACHKUI_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6598144.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6321..7499)
                     /gene="hybB"
                     /locus_tag="ACHKUI_001949"
     CDS             complement(6321..7499)
                     /gene="hybB"
                     /locus_tag="ACHKUI_001949"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6598145.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7489..8475)
                     /gene="hybA"
                     /locus_tag="ACHKUI_001950"
     CDS             complement(7489..8475)
                     /gene="hybA"
                     /locus_tag="ACHKUI_001950"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6598146.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8478..9596)
                     /gene="hybO"
                     /locus_tag="ACHKUI_001951"
     CDS             complement(8478..9596)
                     /gene="hybO"
                     /locus_tag="ACHKUI_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6598147.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9785..10072)
                     /gene="yghW"
                     /locus_tag="ACHKUI_001952"
     CDS             complement(9785..10072)
                     /gene="yghW"
                     /locus_tag="ACHKUI_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6598148.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10191..11078)
                     /gene="yghX"
                     /locus_tag="ACHKUI_001953"
     CDS             complement(10191..11078)
                     /gene="yghX"
                     /locus_tag="ACHKUI_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6598149.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11235..12275
                     /gene="gpr"
                     /locus_tag="ACHKUI_001954"
     CDS             11235..12275
                     /gene="gpr"
                     /locus_tag="ACHKUI_001954"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6598150.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12315..12809)
                     /gene="yqhA"
                     /locus_tag="ACHKUI_001955"
     CDS             complement(12315..12809)
                     /gene="yqhA"
                     /locus_tag="ACHKUI_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6598151.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13000..13884
                     /gene="yghA"
                     /locus_tag="ACHKUI_001956"
     CDS             13000..13884
                     /gene="yghA"
                     /locus_tag="ACHKUI_001956"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6598152.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13923..14348)
                     /gene="exbD"
                     /locus_tag="ACHKUI_001957"
     CDS             complement(13923..14348)
                     /gene="exbD"
                     /locus_tag="ACHKUI_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6598153.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14355..15089)
                     /gene="exbB"
                     /locus_tag="ACHKUI_001958"
     CDS             complement(14355..15089)
                     /gene="exbB"
                     /locus_tag="ACHKUI_001958"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6598154.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15341..16528
                     /gene="metC"
                     /locus_tag="ACHKUI_001959"
     CDS             15341..16528
                     /gene="metC"
                     /locus_tag="ACHKUI_001959"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6598155.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16668..17327
                     /gene="yghB"
                     /locus_tag="ACHKUI_001960"
     CDS             16668..17327
                     /gene="yghB"
                     /locus_tag="ACHKUI_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6598156.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17367..18266)
                     /gene="yqhC"
                     /locus_tag="ACHKUI_001961"
     CDS             complement(17367..18266)
                     /gene="yqhC"
                     /locus_tag="ACHKUI_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6598157.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18460..19623
                     /gene="yqhD"
                     /locus_tag="ACHKUI_001962"
     CDS             18460..19623
                     /gene="yqhD"
                     /locus_tag="ACHKUI_001962"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6598158.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19728..20555
                     /gene="dkgA"
                     /locus_tag="ACHKUI_001963"
     CDS             19728..20555
                     /gene="dkgA"
                     /locus_tag="ACHKUI_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO6598159.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20755..21681
                     /gene="yqhG"
                     /locus_tag="ACHKUI_001964"
     CDS             20755..21681
                     /gene="yqhG"
                     /locus_tag="ACHKUI_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO6598160.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     PIERQFIYDNPICFGEESLFSRVDEIRVLGKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21732..21989
                     /gene="yqhH"
                     /locus_tag="ACHKUI_001965"
     CDS             21732..21989
                     /gene="yqhH"
                     /locus_tag="ACHKUI_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6598161.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22031..24250)
                     /gene="ygiQ"
                     /locus_tag="ACHKUI_001966"
     CDS             complement(22031..24250)
                     /gene="ygiQ"
                     /locus_tag="ACHKUI_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6598162.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     NELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24502..25251
                     /locus_tag="ACHKUI_001967"
     CDS             24502..25251
                     /locus_tag="ACHKUI_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6598163.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25574..27046
                     /locus_tag="ACHKUI_001968"
     CDS             25574..27046
                     /locus_tag="ACHKUI_001968"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6598164.1"
                     /translation="MSGNESIDAAHYDQVTTWPRGGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKATGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKV
                     SAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            27043..28059
                     /locus_tag="ACHKUI_001969"
     CDS             27043..28059
                     /locus_tag="ACHKUI_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO6598165.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28070..29080
                     /locus_tag="ACHKUI_001970"
     CDS             28070..29080
                     /locus_tag="ACHKUI_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO6598166.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLIAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            29153..30136
                     /locus_tag="ACHKUI_001971"
     CDS             29153..30136
                     /locus_tag="ACHKUI_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO6598167.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            30178..30660
                     /locus_tag="ACHKUI_001972"
     CDS             30178..30660
                     /locus_tag="ACHKUI_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6598168.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            30671..31975
                     /locus_tag="ACHKUI_001973"
     CDS             30671..31975
                     /locus_tag="ACHKUI_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6598169.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(32046..33458)
                     /gene="ftsP"
                     /locus_tag="ACHKUI_001974"
     CDS             complement(32046..33458)
                     /gene="ftsP"
                     /locus_tag="ACHKUI_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6598170.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(33533..34270)
                     /gene="plsC"
                     /locus_tag="ACHKUI_001975"
                     /gene_synonym="parF"
     CDS             complement(33533..34270)
                     /gene="plsC"
                     /locus_tag="ACHKUI_001975"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6598171.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(34505..36763)
                     /gene="parC"
                     /locus_tag="ACHKUI_001976"
     CDS             complement(34505..36763)
                     /gene="parC"
                     /locus_tag="ACHKUI_001976"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6598172.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(36901..38508)
                     /gene="ygiS"
                     /locus_tag="ACHKUI_001977"
     CDS             complement(36901..38508)
                     /gene="ygiS"
                     /locus_tag="ACHKUI_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6598173.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(38617..39099)
                     /gene="ygiV"
                     /locus_tag="ACHKUI_001978"
     CDS             complement(38617..39099)
                     /gene="ygiV"
                     /locus_tag="ACHKUI_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6598174.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            complement(39152..39544)
                     /gene="ygiW"
                     /locus_tag="ACHKUI_001979"
     CDS             complement(39152..39544)
                     /gene="ygiW"
                     /locus_tag="ACHKUI_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6598175.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            39696..40355
                     /gene="qseB"
                     /locus_tag="ACHKUI_001980"
     CDS             39696..40355
                     /gene="qseB"
                     /locus_tag="ACHKUI_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6598176.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            40352..41701
                     /gene="qseC"
                     /locus_tag="ACHKUI_001981"
     CDS             40352..41701
                     /gene="qseC"
                     /locus_tag="ACHKUI_001981"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6598177.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAEQGGFEAKVSW"
     gene            complement(41747..>42001)
                     /locus_tag="ACHKUI_001982"
     CDS             complement(41747..>42001)
                     /locus_tag="ACHKUI_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO6598178.1"
                     /translation="FAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTPLII
                     LILRKLFMREMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT        10488 a        10901 c        10421 g        10191 t
ORIGIN      
        1 ccccgtactg attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
       61 accacaatta acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc
      121 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
      181 tccactttcg gcaaacacca cagttgctgg taaatatttt tctgttcggc aaatttacca
      241 ctggtctgat caagcacctc ttcatgatgg ctgaccaggt caatgttgct accacagcgc
      301 ccggcaatcg gtttaacggc ataaccggtt ttcaccagct catcattcac ggtgaagtca
      361 gtatccaaca gataacgatg gtgcgggaac agagaccaga ggatcggcag aatcgctttg
      421 ttaccgggga tcaccgtcca tagcggctca aaaaccaaca cttccgggcg cagcaatacg
      481 tcgataagac gcacttcatt ttgtggatgc ccggtacgga tcggcactgc ggcaaattca
      541 cggtcgctca cttcgcggat ttgatcaaat gccgtttccc acgcccaggt tttccatacg
      601 cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
      661 tccaacccac gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
      721 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
      781 cgactgtgtt tccacgcgcc agccagttcg ttaatcagtc cttccgcagg attgaagccg
      841 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacag
      901 gaggcagaat cggcgttgta ttcgtagact ttcaggccac gttcatccat gcagaaatcc
      961 atacgaccag tgatcatatg atgacggcga cgctgccagg agagacgcaa acgtggccag
     1021 aggattttcg gaatgtcaaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtg
     1081 gcgtgaagat acatcaggtg cagctcgttg gtggctttaa tcagctcctg ctcggcactc
     1141 tcggtaatgg tgtagtaatg atacggatcc tgattgatca cctggccgtt ggcctgtaca
     1201 taggcgtttt gcagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
     1261 tccagacgcg ccccgctgat tttcagcagt tcgcctgcaa tttccggctg tggcaagctg
     1321 tattcggtat cttccgtctg gatcatccag cccagaatag tggtgtcatc gaaggtgtct
     1381 ttcagggtat agccgccgtt ttcgaccacc atctccagct cgcgtgtcca ctgttgccct
     1441 tgcggcaacg gggtatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
     1501 tgggtaatga tggcgacatg accagtgtct ttaaattcac cgcctttatc ccagataaga
     1561 agcgcccccg ctaccggtgc acgcggcgaa ccgttaggaa atgcctgcaa tggcaggatg
     1621 ttgtcattga ccacttcacg caggaagcgc agtgagaaaa tctcccatgc catacccacg
     1681 tcagtgaaga ctacaccgta gttcagaaag agaaaacggc gagcaaattc aacgcattgc
     1741 catttgtggc ccatatattc atcgtcgata tagctacgga atacggcgtc atcttcgtat
     1801 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
     1861 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
     1921 ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac
     1981 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
     2041 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
     2101 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
     2161 aatccgctgg cggcgcgttc gccagtatca atcgcccggt ttctggtccg acgcatgaaa
     2221 aaacgctgcc ggtgggcaaa catccattgc aactttattc gctgggaacg ccgaacggtc
     2281 agaaagtaac gattatgctt gaggagctgc tggcactggg cgttactggt gcagagtacg
     2341 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
     2401 acccaaactc gaagatcccg gcgctgcgcg accataccca taatccaccg atccgcgtgt
     2461 ttgagtctgg atcgattctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
     2521 atttggcaaa gcgtactgaa acgatgaact ggctgttctg gttacagggc gcggcaccgt
     2581 tcctcggcgg tggttttggt cacttttacc attacgcgcc ggtgaaaatt gagtacgcca
     2641 tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt gctggataag caactggcgc
     2701 agcacaagtt tgttgcgggc gatgagtaca ccattgcgga tatggcgatc tggccgtggt
     2761 ttggcaacgt ggtgttaggc ggcgtgtatg atgccgcaga gtttctggat gcgggcagtt
     2821 ataagcacgt gcaacgctgg gcgaaagaag tgggcgaacg tccggcggtg aagcgtgggc
     2881 gtattgttaa ccgcaccaac gggccgctga acgagcagtt gcatgagcgc catgacgcca
     2941 gtgatttcga gacgaatacg gaagataagc gtcaggggta agtcgcaaca ttggcaggct
     3001 tatcgctatg tagccggata aagcatttac gccgcatccg gctttaatca tcacgcgctg
     3061 gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg
     3121 atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg
     3181 ttaccttcac aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc
     3241 gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt
     3301 gttaactcca gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca
     3361 cgcaaccgct cgccatgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc
     3421 tggctgcaat cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct
     3481 tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg
     3541 caggagagcg cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc
     3601 gcctgccgtt ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc
     3661 actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattagcgac
     3721 cggtagcgaa aggatcatcc gcgcgcagtc atccgtcagg cgttggccct cttcaatcga
     3781 catgctatgc gaaagcggtg acatcagcga acaggagaga tattgagaaa cactgtccag
     3841 ttcgccaacg gtaaaggtca tagtgccata cggcagttgc agaccaattt tttcactcac
     3901 tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg
     3961 ggtgatcaca caccccgtcc actgaccttc gaacagcgta aagtcagaaa catacaccgg
     4021 cattgaggga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc
     4081 tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgagtcg
     4141 cctcccgtgg gatagcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg
     4201 caggttcaat cattgcttca accgtcggcg ttaagccgat gtgcggctcc agcgattccg
     4261 ggatcacacc gaccagcgtc agctttttcg gaaactcgcc ggtgaagcgc agggccgaca
     4321 atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt
     4381 cttcatcccg caggatcatc atcgttcccg gcgcgttctt tttcgacaca atggcatccg
     4441 caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc
     4501 catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcaacaatcc
     4561 gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca
     4621 ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca
     4681 ggccatgcac gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc
     4741 ggcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg
     4801 cggaccagag ttccaggttg atggaacaac cgcctggtag ttgctgatga taccgtcttt
     4861 aataaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa
     4921 ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt tgccgatatt
     4981 ggtgatcagt gcactgtatt ggttttgcag gatatcctgc aattcacagc agtgaacggt
     5041 acgaccaata atacggccca gcgtggagtg cagctgtgcc acttccagcg tgttgccagt
     5101 cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc
     5161 cgccagtttc accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt
     5221 cggcgatttc acccaggaat atttaccgtc atcagaccaa ccgtcgtaag ccggaatggt
     5281 ggtaccttcc cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc
     5341 ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag
     5401 atccgcattc tcaatgtagc cgcccgggaa caggaagctg ccgtttttgc tgtcggtcgg
     5461 gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta
     5521 gaacgcggcg ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt
     5581 gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag
     5641 gttgattggg ttagcgaccc cgcctaccgc caggttctga atgtgcggcg ttttaccgcc
     5701 cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc
     5761 taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt
     5821 ggcgaaaata cccaactgac cgctggcaac cagatcttta atcttgttct gaactttggt
     5881 gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt cagaggcttt
     5941 ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata
     6001 gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg
     6061 cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc
     6121 gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc
     6181 gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga
     6241 aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg
     6301 atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt
     6361 tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata
     6421 gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg
     6481 cccgggttga atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg
     6541 ctcagtgctg acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg
     6601 accagcggga agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg
     6661 gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa
     6721 atcgccagca acacactgat ggtgttggtc agcttgacga acaggctctt ttcatccgga
     6781 ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa agatgacaat cgagaagccc
     6841 atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacagcgga
     6901 tgcaccttgt agcccgccga gatcatcagc gaccccattg aagactggtg catggtcggc
     6961 agcagcgcac cgagcgcgat gatgaagaac atcaccttgt tcagacgctg tagcgacacc
     7021 ttccagccca gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag
     7081 atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg
     7141 tagaagtacg gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacctagt
     7201 gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg
     7261 ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccgatc
     7321 aacaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag
     7381 cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca
     7441 aaaatcatga ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg
     7501 atcgtctcct ccgtcgtgat ggtcattttt ggtgttgcga cgaaccagca cggttaagcc
     7561 cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga
     7621 acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc
     7681 cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata
     7741 tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta
     7801 ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt
     7861 accaaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc
     7921 gagacgttcc acacctttct ggttgcacaa ctcgcactta tgcagagcac caaacgggtt
     7981 gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc atgcagtaac ggcagccggt
     8041 gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gcgcagagac
     8101 cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc
     8161 gtagccattc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat
     8221 gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt
     8281 acgttcaggg gagttgatat cctgacactt ggtgacgcaa gcctgacagc ctacgcacaa
     8341 ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc
     8401 atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa
     8461 attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga
     8521 cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac tccgccgagc
     8581 aaaccaatag cgcctgccga gacgttgccg ccttctttag cgttaacgtc cggtttctgt
     8641 gaacgcggag tctggttttc gacgttggca agctgatgga tgcctttatg gaagccgata
     8701 ccttcttcgt tacagccata gcacgggtga ccaatcgcta ccggccacac gccgccaaca
     8761 tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg
     8821 tacaggcacc agccttcgcg gtggccttca tcaccgaact ctttggcaaa acgaccagca
     8881 tcgaagtgcg ggcgacgttc acagtgttcg tgaatcagac gaccataggc gaaagtcgga
     8941 cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcc
     9001 aggaagttgt gcgggttcgg cgggcagcca ggaatattga taacggtttt gcccggtaga
     9061 acttcttgca ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac
     9121 gcagagcagg aaccgatagc gataatggct gctgcgcctt ccgccgcttt gcggatgtga
     9181 tccacaatcg gctcaccggc aaccatgcaa taaataccgt tatcttttaa tgggatggaa
     9241 ccatccacca ctaacacata ctgccctttg tacttctcga gagcgttatg tttgttctct
     9301 tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc
     9361 agcacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg
     9421 cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcgacc
     9481 atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc
     9541 atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat
     9601 agttattccg ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg
     9661 atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg
     9721 gcactaaaac gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt
     9781 ttgatcaatt ttctaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat
     9841 tctcacgaaa gaaatccatt accatctctt tatccagtcc ataacggcgc gtcagaatac
     9901 ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc
     9961 ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg
    10021 catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc
    10081 tcattcgcta tatattgtca tatatagcga tattttaggc aaagaacgac atttgcgcac
    10141 gctatttttt catgcttgtt taagacgtaa tcctgtaaga gataaaccca tcaggagaga
    10201 tatttatcga accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa
    10261 cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatatatata cgcctcatag
    10321 accttattat tggctttcaa cgccgcctcg taagcgggcc agccctcgtt gattcggcta
    10381 tccagttcag cgtagtggag cagtaaaggc gcttcaatct tcgccacatc ggcagtgggt
    10441 gcctgacgcc cataaaacgg caccgcgcag gccagttccg gatacgcgac tgctgccgcg
    10501 ttcgatacgc ccccgccata gcaaaatccg ctaataccca ctttgcctgt cgcttgcgga
    10561 tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc
    10621 tgctgttgca gttcgcggcc tttatcatcg ttaccaggat agccacctac tgagcttaaa
    10681 ccgtctggcg ccagggcgat gtacccagcc ttcgccactc gccgcgtcac atcttcgata
    10741 tacggattca gccctcgatt ctcatgcacc actaccacgg ctggcgtttt gccgctcatt
    10801 tttgcgggct tcaccagata accccgtacc tcgccgtgac catttggcga gggatacgtg
    10861 atgtactcag caacaatttc cgggtcggta aactctacct gagtcgccaa cgcataattt
    10921 ggcttgagca aatcaaacag cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca
    10981 aggttgagga actcacgttt cgagattttc ccgtgagcgt aatagtcgta gtaatccagc
    11041 aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg ttgcactcct caactggtgt
    11101 ttttttaagc aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag
    11161 actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacgat
    11221 ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcagtacc
    11281 gctattgcgg aaaaagtggt ttacgcctgc ccgcgttatc gctcggttta tggcacaatt
    11341 tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct gcgtaaagcg tttgatttag
    11401 gcattacgca ctttgattta gccaacaact acgggccgcc tccaggaagc gcagaagaga
    11461 actttggtcg cctgctgcgc gaggattttg ccgcttatcg cgatgaactg attatctcta
    11521 ccaaagccgg atacgatatg tggcccggcc catacggctc tggcggctca cgtaaatacc
    11581 tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt
    11641 actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg
    11701 ctgtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc
    11761 aaaaaatggt cgagttgctg cgcgagtgga aaattccgct gttaattcat caaccttcgt
    11821 acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg
    11881 gcgtgggctg cattgccttt actccactgg cacagggatt gctgaccgga aaatatctca
    11941 acggtattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc
    12001 cgaaaatgct caccgaagcc aacctcaaca gcctgcggtt attgaatgaa atggcacagc
    12061 agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga
    12121 cgtcggtatt gattggtgcc agccgcgcgg agcaacttga ggagaacgtg caggcgctga
    12181 ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg
    12241 agctgaatct gtggcaggcg tcttccgata aatgacctgt taataacggg cgcgagaacc
    12301 gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat
    12361 gcagagagca caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca
    12421 tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct
    12481 gctactttgt ttttcagcga cgttgcatcc attttcccca gccagttcag cttctctttg
    12541 ttctcggaga tatccagttg tgagacgaaa ttctcgtaac cggaaaacat caccatcacc
    12601 agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat
    12661 tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc
    12721 agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca
    12781 tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgagcaac
    12841 agtatatcgc aattaagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt
    12901 gtcgcataat tctgagggtt agggatcttc ctggttccct tatcaacgca tcgagagcgt
    12961 ctacccttaa aggacgttga acctgaaggg agaaaaatga tgtctcattt aaaagacccg
    13021 accacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag
    13081 gcgaagatga caccggtacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg
    13141 aaagatcgta aagcactggt gacagggggc gattccggaa tcggtcgcgc tgccgccatt
    13201 gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac
    13261 gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc
    13321 gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcacacaa ggcgttaggc
    13381 gggctggata ttatggcgct ggtagccggg aaacaggttg ccattcctga tattgcagac
    13441 ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgtt gttctggcta
    13501 acccaggaag cgatccccct gctaccgaaa ggtgcaagta tcatcaccac ttcctcaatc
    13561 caggcatacc agccaagccc gcatttactg gactatgcgg ctacgaaggc ggcaattctg
    13621 aactatagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc
    13681 gcgccaggcc cgatctggac agcactgcaa atttctggcg gacaaacgca ggataagatc
    13741 ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga gctggcccct
    13801 gtatatgttt acctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggtgtc
    13861 tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat
    13921 ttttatttcg ctttcgcagt ttcttcgccg accagaccta ttttcaggta acccgcctga
    13981 tgcagcgtat ccattacctt catcaacgtc tcgtaatcga cggttttatc cgctcggaag
    14041 aagatggtgg tgtctttctt gccttcggtt aacgcattca acgccgtaat cattgtttca
    14101 tcggtgaccg gatcgttacc aataaacatc gagttgtctg ctttcaccga cagataaacc
    14161 ggtttttccg ggcgcggctg cggcgtactg gtagaagcag gcaaattcac cttcacatct
    14221 accgtcgcta acggtgccgc caccataaag ataatcagca aaaccaacat cacgtcgata
    14281 aacggcgtca cgttgatatc atgcatttcg ccgttatcgt cgaggttttc gttaagatgc
    14341 atcgccatcg gacattatcc tgcgcgtaat ttttgtgcga cacgaaccgg atgtgcagcg
    14401 gcgctggctt ccagatccag gtcacggctt tgcagcaaca atacctgcgc tgcaacatca
    14461 cccagcatcg ctttaaagcc gccaatctgg cgtgcaaata cgttatagat aacgaccgca
    14521 ggaatcgctg ccacgaggcc gattgccgtt gctaacagag cttctgcaat acccggcgca
    14581 acgactgcca ggttagtggt ctgcgtttgc gcgataccaa taaagctgtt catgatgccc
    14641 cagaccgtac caaacagacc aacgaacggc gaaatcgcgc cgatggttgc cagatagccg
    14701 ttaccacggc ccatttgacg acctactgcg gcgacccgac gctccagacg gaagctggta
    14761 cgttctttaa taccttcgtt atcgtcgctg ccttctgaca gttccagctc attctgcgct
    14821 tcattgagca aatgcaggct caagcttttg ctaccaaaat cagcggcgat atcgttggcc
    14881 tggtttaagg aacgcgcttc agccagcaat tgctgctcgc gcttaagacg acgcttctga
    14941 ttgaagaact ccacgctctt actgaagaag attgcccagg tgactacgga ggccaaaata
    15001 agcccaatca tcacgcactt aacgacaata tcggcgtgct gatacatacc ccagacggag
    15061 aggtccgtct gcattaaatt attacccact gtgtatctcc aggacgcaag tcacaaaatc
    15121 tgtgcataat aatatcaaaa cgacgtcgaa ttgatagtcg ttctcattac tatttgcata
    15181 ctgccgtacc tttgctttct tttccttgcg tttacgcagt aaaaaagtca ccagcacgcc
    15241 atttgcgaaa attttctgct ttatgccaat tcttcaggat gcgcccgcga atattcatgc
    15301 tagtttagac atccagacgt ataaaaacag gaatcccgac atggcggaca aaaagcttga
    15361 tactcaactg gtgaatgcag gacgcagcaa aaaatacacg ctcggtgcgg taaatagcgt
    15421 gattcagcgc gcgtcttcgc tggtctttga cagtgtggaa gccaaaaaac acgcgacacg
    15481 taatcgcgcc aatggcgagt tgttctatgg acggcgtgga acgttaaccc atttctcctt
    15541 acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgt gtgctatttc cctgcggggc
    15601 ggcagcggtt gctaattcca ttcttgcttt tgtcgaacag ggcgatcatg tgttgatgac
    15661 caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa atcctcagca aactgggcgt
    15721 aacgacatcg tggtttgatc cgctgattgg tgccgatatc gttaagcatc tgcagccaaa
    15781 cactaaaatc gtgtttctgg aatcgccagg ttccatcacc atggaagtcc acgatgttcc
    15841 ggcgattgtt gccgccgtac gcagtgtggt cccggatgcc atcattatga tcgacaacac
    15901 ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc atcgatgttt ctattcaggc
    15961 cgccaccaaa tatctggttg ggcattcaga tgcgatgatt ggcactgcgg tgtgcaatgc
    16021 ccgttgctgg gagcagctgc gagagaatgc ctatctgatg ggccagatgg tcgatgccga
    16081 taccgcctat ataaccagcc gtggcctgcg cacattaggt gtgcgtttgc gccaacatca
    16141 tgaaagcagt ctgaaagtgg ctgaatggct ggcagaacat ccccaagtag cgcgagttaa
    16201 ccaccctgct ctgcctggca gtaaaggcca cgaattctgg aaacgagact ttacaggcag
    16261 cagcgggcta ttttcctttg tgcttaagaa aaaactcaat gatgaagagc tggcgaacta
    16321 tctggataac ttcagtttat tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat
    16381 cctggcaaat caaccagaac atatcgccgc cattcgccca caaggcaaga tcgattttag
    16441 cgggaccttg attcgcctgc atattggtct ggaagatgtc gacgatctga ttgccgatct
    16501 ggacgccggt tttgcgcgaa ttgtataaca ttgccacttt tggacaattt tgcagacatt
    16561 ttattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag gcgtcccgga cgattcagga
    16621 gtacaatagg cagataaagg cttaaacgct gttccacagg aaagtccatg gctgttattc
    16681 aagatatcat cgctgcgctc tggcaacacg actttgccgc gctggcggac cctcatattg
    16741 ttagcgttgt ttactttgtt atgtttgcca cgctgttttt agaaaacggc ttgctgcccg
    16801 cctcattttt gccaggcgac agcttgttga tactggcagg cgcattgatt gcccaggggg
    16861 ttatggattt tctgcctacg attgcgattc tgaccgccgc agcaagtctg ggctgctggc
    16921 taagttatat tcaggggcgc tggttaggga ataccaaaac ggtgaaaggc tggctggcac
    16981 agcttcctgc taaatatcac cagcgcgcca cctgcatgtt tgaccgccac ggtctgctgg
    17041 cgctgctggc tggacgtttt cttgcatttg tccgtacgct gctgccaacc atggcgggaa
    17101 tttccggtct gccaaaccgc cgcttccagt ttttcaactg gttaagcgga ttgctgtggg
    17161 tcagcgtggt aaccagtttc ggctatgcct taagtatgat tccgttcgtt aaacgacatg
    17221 aagatcaggt aatgacgttc ctgatgatcc tgccaattgc cttgttaacc gctggcttgt
    17281 tgggcacgct gtttgtggtg attaaaaaaa aatactgtaa cgcctgacga ttttccccgt
    17341 tcccggttgc tgtaccggga acgtatttaa ttcccctgca tcgcccgcat tcttgccgca
    17401 tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac ggctaaattg cgatgcgctt
    17461 tcatagccga cgcgcatcgc cgccgcgctg gccttcatgc cgtcgtggat gatcatcatc
    17521 cgcgccttat gcagacggta attcttcaaa tactgcaacg gcgaggtgct ggtgacagac
    17581 ttaaaattat ggtggaacgc cgatacgctc atgttggctt ctgccgccag ttgctcgacg
    17641 ctcaggtttt cggtgtattt attctcaatc cgtttcagca cgcgactaat caggctgaag
    17701 tgagtctggc ggctgaccag tgccagtaac gcgccgccgc aaggtccggt cagcacgtag
    17761 tacagaattt cgcggataat ctgtttgccg agaatgcgcg catccagcgg tcgctccatc
    17821 acatccagta accgctctgc cgcgcataaa atctcttctg ataacgtggc ggagttaata
    17881 ccgctggctg ccattgacgg ctgaaaatgt tcatcttcgc caatgtccat caacagttcc
    17941 tgtaactgca aaatatcgac attgagacgc aaccctgcca gcggcacctc tgacgtggca
    18001 taggtttcgc actcaaacgg caacggcacc gtcagcagga gatattcatt agcgtcataa
    18061 cgaaacacgc gttcattgat ataaccgatt ttatgtccgg aaaagagaat tatgatgcca
    18121 ggctcgtaca tcaccggtgt gcgtgcgaaa ggcgtctcgc catacaacaa acgcacatcg
    18181 ggcaacagct ctgacaaact attttcttta tttttcagtt tattaacttt atccgccagc
    18241 aagcggcaaa tctcttcacg tttcatatcg cgtaatttct taggaataat gcggcaattt
    18301 gattgtgcgc aattttgtag catttctcca gcactctgga gaaataggca agacattggc
    18361 agaaatgagc attgagaggt acggcgctgg cgaccacaat gaaaaacatc aggcagaccg
    18421 atctctgccc tcatattgac ccagcaaagg gagcaagtaa tgaacaactt taatctgcac
    18481 accccaaccc gcattctgtt tggtaaaggc gcaatcgctg gtttacgcga acaaattcct
    18541 cacgatgctc gcgtattgat tacctacggc ggcggcagcg tgaaaaaaac cggcgttctc
    18601 gatcaagttc tggatgccct gaaaggcatg gacgtgctgg aatttggcgg tattgaacca
    18661 aacccggctt atgaaacgct gatgaacgcc gtgaaactgg ttcgcgaaca aaaagtgact
    18721 tttctgctgg cggttggcgg cggttctgta ctggacggca ccaaatttat cgccgcagcg
    18781 gctaactatc cggaaaatat cgatccgtgg cacattctgc aaacgggcgg caaagagatt
    18841 aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag caaccggttc agaatccaac
    18901 gcaggcgcgg tgatctcccg taaaaccaca ggcgacaagc aggcgttcca ttctgcccat
    18961 gttcagcccg tatttgccgt gctcgatccg gtttatacct acaccctgcc gtcgcgtcag
    19021 gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg aacagtatgt taccaaaccg
    19081 gttgatgcca aaattcagga ccgtttcgca gaaggcattt tgctgacgct gatcgaagat
    19141 ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc gcgccaacgt catgtgggcg
    19201 gcgactcagg cgctgaacgg tttgatcggc gctggcgtac cgcaggactg ggcaacgcat
    19261 atgctgggcc acgaactgac tgcgatgcac ggtctggatc acgcgcaaac actggctatc
    19321 gtcctgcctg cactgtggaa tgaaaaacgc gataccaagc gcgctaagct gctgcaatat
    19381 gctgaacgcg tctggaacat cactgaaggt tcagacgatg agcgtattga cgccgcgatt
    19441 gccgcaaccc gcaatttctt tgagcaatta ggcgtgccga cccacctctc cgactacggt
    19501 ctggacggca gctccatccc ggctttgctg aaaaaactgg aagagcacgg catgacccaa
    19561 ctgggcgaaa atcatgacat tacgctggat gtcagccgcc gtatatacga agccgcccgc
    19621 taagcttttt acgcctcaaa ctttcgtttt cgggcatttc gtccagactt aagttcacaa
    19681 cacctcaccg gagcctgctc cggtgagttc atataaagga ggaacgtatg gctaatccaa
    19741 ccgttattaa gctacaggat ggcaatgtca tgccccagct gggactgggc gtctggcaag
    19801 caagtaatga ggaagtaatc accgccattc aaaaagcgtt agaagtgggt tatcgctcga
    19861 ttgataccgc cgcagcctac aagaacgaag aaggtgtcgg caaagccctg aaaaatgcct
    19921 cagtcaacag agaagaactg ttcatcacca ctaagctgtg gaacgacgac cacaagcgcc
    19981 cccgcgaagc cctgctcgac agcctgaaaa aactccagct tgattatatc gacctctatt
    20041 taatgcactg gcccgttccc gctatcgacc attatgtcga agcatggaaa ggcatgatcg
    20101 aattgcaaaa agagggatta atcaaaagca tcggcgtgtg caacttccag atccatcacc
    20161 tgcaacgcct gattgatgaa actggcgtga cgcctgtgat aaaccagatc gaacttcacc
    20221 cgctgatgca acaacgccag ctacatgcct ggaacgcgac acacaaaatc cagaccgaat
    20281 cctggagccc attagcgcaa ggagggaaag gcgttttcga tcagaaagtc attcgcgatc
    20341 tggcagataa atacggcaaa acccctgcgc agattgttat ccgctggcat ctggatagcg
    20401 gcctggtggt gatcccaaaa tcggtcacac cttcacgcat tgtcgaaaac tttgatgtct
    20461 gggatttccg tctcgacaaa gacgaacttg gcgaaattgc aaaactcgat cagggcaagc
    20521 gtctcggtcc cgatcctgac cagttcggcg gctaacgtgc aaattatccc ggtggcggta
    20581 atgttccgct accggacttt tcagaaatca tttattcccc tcgcgtcccg cccgttgcta
    20641 ctcttccttg ttcaggaatg ccaaatataa ggacatcatc atgcagagcc ggaagctctt
    20701 aaaagaacaa cccatctata tccgggataa acgcaacgga gaggtgaaaa acagatgaaa
    20761 ataatacttc tgtttttagc agccctggca agttttaccg tacacgcaca gcccccctca
    20821 cagaccgtag aacaaacagt ccggcatatt tatcagaact ataaatcaga tgccactgcc
    20881 ccttattttg gtgaaaccgg agagcgggcg ataacatctg cgcgtattca acaggcgctt
    20941 accctgaacg acaatcttac gctgccgggc aatattggct ggctggatta tgatccggtt
    21001 tgtgattgtc aggattttgg cgatctggtg ttagaaagcg tagcgataac ccaaactgac
    21061 gccgatcatg ccgatgccat tgtgcgcttt cgtatctttc aagacgataa agaaaagacc
    21121 tcgcagacac tgaaaatggt ggcggaaaat ggtcgttggg tcattgacga tattgtcagc
    21181 aatcatggca gcgtcttaca agcggttaat agcgagaatg aaaaaacgct ggccgctata
    21241 gcttcgttgc aaaaagaaca gccggaagcc tttgttgccg aactctttga acatattgct
    21301 gattatagct ggccgtggac gtgggtggtt tccgactctt accgccaggc ggtcaatgcc
    21361 ttctataaaa ccaccttcaa gacggccaac aatcccgatg aagatatgcc aatagaacgg
    21421 caatttattt acgacaatcc gatctgtttt ggcgaagagt cgttattttc ccgcgttgat
    21481 gaaattcgag tcctggggaa aaccaccgat tccgcccgca ttcatgttcg ctttacgctg
    21541 accaatggca acaacgaaga gcaagaactg attttacagc ggcgcgaagg taagtgggaa
    21601 atcgctgatt ttatccgccc gaacagcggc agcctactta agcagattga ggcaaaaacc
    21661 gccgccagat taaaacaatg agctgaatta aataacaatt agccggaaca ataaataaaa
    21721 gggaacacta tatgaaaacg attttcaccg tgggagctgt tgttctggca acctgcttgc
    21781 tcagtggctg cgtcaatgag caaaaggtca atcagctggc gagcaatgtg caaacattaa
    21841 atgccaaaat cgcccggctt gagcaggata tgaaagcatt acgcccacaa atctatgctg
    21901 ccaaatccga agctaacaga gccaatacgc gtcttgatgc acaggactat ttcgactgcc
    21961 tgcgctgctt gcgtatgtac gcagaatgat aaaaaaatcc ccggcagcat tctgttaccg
    22021 gggatatttt ttaacgtcca accgccgctt tagggcgttt cttcgcacca gcattctgca
    22081 ggcgagattg cgtagacgac gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca
    22141 tcggcgtatg tttagtcaac gccggacggg tattgcggtt ctgacggcga gcttcacgca
    22201 tctcttcaat ggttggcgca ggcactaagc aatcgcgacg gctgccaatc agatgctttt
    22261 tgcccatcgc ttccagcgcc tggcggatta acggccagtt tgccggatcg tggtaacgca
    22321 acaacgcttt atgcaggcga cgctgcttgt cgcccttcgg tacgaagacg tcttcactct
    22381 tataaccaat cttcgccagc gggtttttgc cggtgtaata catggtggtc gagttagcca
    22441 gcggcgacgg atagaagttc tgcacctggt cgaggcggaa gcgatgcttt ttcagccata
    22501 gcgccagatt caccatatct tcatcacgcg tacccgggtg cgcggagatg aaatacggga
    22561 tcagatactg ttctttacct gcctgtttcg agtaagtatc gaacagctct ttaaagcggt
    22621 catagctgcc catgcccggc ttcatcatct tcgataacgg cccttcttcg gtatgttccg
    22681 gggcaatctt cagataaccg ccaacgtgat gggtcgccag ctcgttgata tagcgcggat
    22741 cttctacggc gatgtcataa cgcacaccag aggcaatcag gatcttttta atgcctttca
    22801 gatcgcgcgc acggcgatag aggttgatcg tcggttcgtg gttagtgtcc atatgcggac
    22861 aaatatccgg ataaacgcac gacaaacgac ggcaggtttg ctcagcgcgt ggcgatttgc
    22921 aacgcaacat atacatgttg gcagtaggcc caccgagatc ggaaatcacg cccgtaaaac
    22981 ctggaacagt gtcgcggatc gcttcgatct cattaataat cgaatcttcg gaacggctct
    23041 gaataatgcg cccttcgtgc tcggtaatag aacagaaaga gcagccgcca aagcagccac
    23101 gcataatgtt gaccgagaaa cgaatcattt cgtaagccgg aattcgggca ttaccgtaag
    23161 ccggatgcgg cacgcgcttg tacggcagcg caaagacgct atccatctct tcggtagaaa
    23221 gcggaatagc aggcgggttg atccatacat agcggtcgcc atgtttttgc atcaatgcgc
    23281 gggcgcagcc tgggttagtt tcgtggtgca gaatgcgcga agcatgggcg tacagcactt
    23341 tatcgccctt cactttctcg aaagaaggca gcaacacgta ggttttttcc cacggtttcg
    23401 ggcgtggtgg ctgcacggtt acagctttgg cttcctgctt tttcggagct accggtttgt
    23461 tatccgcgca cggcaaatct tcgccatacg gatgcgggat tgggtcgatt ttccctgggg
    23521 tatcaagacg ggtggaatcc acaccgctcc agcctggcaa cgcctctttt acgataatcg
    23581 cggtattacg cacatcgcgg atttcactaa tcggctcgcc catcgccaga cggtgcgcca
    23641 cttccaccag cggacgctca ccgttaccaa acatcagcat gtcggctttc gaatccacca
    23701 gcacggaacg gcgcacggta tccgaccagt aatcatagtg cgcggtacgg cgcaggctgg
    23761 cctcaatacc gccgaggatc accggcacat ctttccacgc ctctttacaa cgttgggtat
    23821 aaaccagtgt ggcgcgatcc ggacgcttac ccgcgacgtt atccggtgtg taggcatcgt
    23881 catgacgtaa acggcgatcg gcggtatagc ggttgatcat cgagtccatg ttgccagcag
    23941 taacaccgaa aaacagattc ggtttaccca gacgcataaa gtcgtcttta ctgctccagt
    24001 ctggctgggc gatgatcccg acgcgaaagc cctgcgcttc cagcatacga ccgcaaatcg
    24061 ccatcccgaa gcttgggtga tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc
    24121 tatcccagcc aagttgatcc atctcttcac gtgacatcgg caaaaacggt gccggtccaa
    24181 aacaggcggc ccagtactgc ggccaggaga acaggtcgcg atccggttgg atcagggaga
    24241 tagagctcat tttgcttcca gaaatggtaa aaaaataatc aaaggccggg gattatacgc
    24301 tgtatcacgc gcggaaatga agtgatgttg atgaacggtg gcgaaatacc ctggtgaggt
    24361 ttttcactgc ccgacttggg caagcacatt taaggctggc ggtatcgtgg caactgccgc
    24421 tactatttat gccgatcagc aacgttcaaa tcatattgca cgacgatcgg ctatccgagg
    24481 acacccacag taaggcaagg tatggaacag gttattacaa agcgccgcta ttacgatatt
    24541 ggtctacaga ttgaagagct gctgtattca ggcgtattta aggccggaga acgcttacct
    24601 tcagaacgtg aactgagcga gcggtttaat accagccgaa ctaccattcg tgaagccatc
    24661 attatgctgg aactgaaagg cgtattgaat gtgaagcaag ggtcagggat tttctttgtc
    24721 gactccaccg ataagctcaa ccagaaatca ttaatgccct attcagaaat tggcccgttt
    24781 gaactgttac aggcacgtca ggtaattgag agcaatatta ccggttttgc tgccagccag
    24841 atcagcttta acgagttaca agagttgaag aaaatcattg gattacagga aaaagccatc
    24901 gctgcggaaa gtgataaatt tgaagacctg gatcaccgat ttcatagcat tattgccgaa
    24961 gcgacgcaaa atcgggtact catcaaacaa gctgctgaat tatggcgggc agttcgtaca
    25021 gaaaaccccc gctggaagaa acttaactac aagtacctgc atgaaaagca tttgcgtctg
    25081 caatggctgg aagatcaccg ggctattttc ctggctctgc aacagaaaga ttcagagctc
    25141 gccagggaag cgtcctggag acatctggaa aacagcaaaa atgaactgat caaaatcttc
    25201 aaacaagacg cttccatcag cgattttgat gactttttct tcgctcgtta accttcatta
    25261 gtccagagct ggcggaaaaa ataacgccgg ctctgccttc cgccctccac tggtacacgc
    25321 tctcttttta attggtctaa taaatcgact atcatcagat atcttagatt agcggcaatt
    25381 atcctaaaaa ctgacctcta aaaaactttc ctttcatttt attcatataa ctcattgata
    25441 cagatataaa taatgccttg ttgttctgtt tcgtactttt acctttctct gcaagcgtga
    25501 tgcatccctc tgtttttggt ctaatgaatt atgtaaattg gtatacacac aagccaccag
    25561 gagtaagagc ataatgtcag ggaacgaaag tatcgatgcc gcccactatg atcaggtaac
    25621 cacctggcct cggggcgggt tacaggcaga tattgtccat attggttttg gcgcttttca
    25681 tcgcggacac caggctgtct acacggatct cactaatcaa ctttcggaca cccgctgggg
    25741 gatctttgag attaacctgt ttggtgatgc tcaactgatc gaaaacttaa atgcgcaaaa
    25801 tgggctgttt tcggttgtgg aaacatctgc atcgcaatct acctcacgcc ttgtgcgttc
    25861 ggtggctggc ggtattcata cccccagaga tggcattgcc gcagcaatcc ataaactggc
    25921 tgaacctcag gtaaaaatcg tttcattaac catcaccgag aaaggttatt gcctcgatcc
    25981 gcaaacacgt tcacttgatc tcaccaatgg attaatccaa cacgatctac aaaacccgga
    26041 tgcgcctctg tcagctattg gcgtgatcgt ctgtgctttg caacaacgta aagcgacagg
    26101 actcgccgct tttagtgtgc tctcctgtga caacttgcca gacaatgggc atctgacgcg
    26161 caatgccgtt ctcggttttg cccgacaact ggaccagccc ctggctcagt ggatcgaaga
    26221 aaatgtctca tttccaggca cgatggtcga tcgcattgtt ccggcaatga ctgaatcgca
    26281 attcgcctta ctggaaacta aaaccggtta tgccgatccc tgcgggatcg tctgcgaatc
    26341 atttcgtcag tgggtgatcg aagataattt tgtgcgcgga cgaccggaat gggataaagt
    26401 cagcgcgatg tttgtcagca atgttcagcc ttatgaagag atgaagttac gcatgttaaa
    26461 tggtagccat tcatttctgg cttataacgg ctcactggct ggctatgagt ttatctggca
    26521 atgcatggaa gacgctaatt ttcgttccat tacccgccaa ctgatgatta atgaacaagc
    26581 ccgaacactt aatccagact taaatatcaa catccaggaa tacgccgacc tgttaattga
    26641 acgctttagc aaccgtaatg ttgcacaccg taccgggcaa atcgctatgg acggttcgca
    26701 aaagcttccc cagcgagcgc tgacgccctg gctgaaattg catcagcaaa aacaaaacaa
    26761 tgctgttctg tcactgctcg ttgctggttg gttgcattat gtcattgatg ctgttgagaa
    26821 aagccagtct gtcgctgatc caatgaatga ccaacttcag gcgctaataa aggaacaaca
    26881 agacgcatgg caacaggcgc tcgcattact gcaccttagc gccatatttg gtgatttaag
    26941 caaccatcag ccatttataa atgaaataaa aatcgccttt gcaaatataa aaaacaaagg
    27001 catcaaggcc accatcagcc aattattatc ggatgagcag aaatgaaaac attaatttgt
    27061 cagcagcctg gcgttatgga atatgtggaa aaggatattc ccacaccagc agataatgaa
    27121 gtgctgttaa aaatcaaagc tgtgggtatt tgtggtactg atattcacgc ttttgccggc
    27181 agacagcctt tttttagcta cccacgtgta ttaggtcatg aaatatgcgc cgaagcggtt
    27241 tcgcgaggca gccagtgcca aacagcacaa tcaggccagc gctattccgt catcccatgc
    27301 attccgtgtg gcgagtgcgc agcctgtcgg gaagagaaaa cgaactgctg cgaacgtgtt
    27361 tcgctgtatg gcgtgcatca ggatgggggt tttagtgagt accttgcggt acgtgaagac
    27421 aaccttgtgc ctctccctga cgaggtcagc gacagtgccg gagcattggt tgaatgtttc
    27481 gccattggtg cacatgccgt tcgtcgggca gagatcaagg ctgaacaaaa cgtactggtg
    27541 attggcgctg ggccaatcgg tctggctacc gcagccatcg ccagggctaa aggggcacat
    27601 gttgttgttg ctgatattga ctgtcaacgt cgccagcacg ttgtggatca tctggcaatt
    27661 aatgtcttcg acccaacaca ggaaggtttt attgccgcgc ttagtgaagt gtttggcggc
    27721 gaactggctt gcgtagtcct ggatgcgacg ggaaataaag cttcaatgag tcatgatgtc
    27781 aatcttattc gtcatggcgg caaaattgtt ttcatcggtt tgtatattgg tgaacttgtt
    27841 attgacgatc cgaccttcca taaaaaagag acaacgttac tcagcagccg caatgccaca
    27901 cgggaagatt ttgcgttggt gattgaactg atgcgcagca ataaaattca cgaaaattta
    27961 atgaaaaacc aggcgttcaa tttctttagt gttggcgaag attaccagcg taacgttgta
    28021 gaaaataaaa atatggtcaa gggtgtgatc actttttaag ggaacaaata tgacaactgt
    28081 ttatgtcagt gaagaaaatt taaaatcact ggtgcaccac aaattacata ccgccgggct
    28141 ggataccgac accacacaac aggtgacaga tgttttagta catgccgata ttaccggtgt
    28201 gcattcacat ggtgttatgc gtgtcgaaca ttattgcacc cgccttgctg ccggagggtt
    28261 aaataaagcc ccgcagttta gcattgagca aatttcaccg tcagtggcca ttctcgactc
    28321 tgacgacgga atggggcatt ccgcattaat tagcgccact gagcacgcta ttaaactggc
    28381 ccagcaagag ggtctcggtt ttgttagcgt taaaaacacg tctcactgtg gtgcgttatc
    28441 ttattttgca gagatgatca ccaacaaagg gttgattgct atcgtaatga cgcaaaccga
    28501 tacctgtgtg gctccccatg gcggcgccga gcgctttttg ggaactaacc ccatcgcctt
    28561 tggtttcccg gtggaaaaca gccatccgat gattgttgat atggcgacca gtgccacggc
    28621 tttcggcaaa atacttcatg caaaggaaac cggaaaacat attggtgaag ggctggcgat
    28681 agataaaaac ggttatggca ccactgatcc gtataagatt gaaaacctgc tacctttcgg
    28741 ccaacacaaa ggttcaggca ttgcactggc tattgatgca ctgactggcg tgctgatgaa
    28801 tgcgaatttt ggcaaccata ttgttcgcat gtatggtgat tatgacaaga tgcgtaagct
    28861 ggcaagtttg gttattgcca tcgatccgaa aaagctcggc aatcctgttt ttgcaaaaac
    28921 tatggcgaaa atggtcacgg agctgcatgc cgttaaaccg gctcctggtg tcgaaaaagt
    28981 gttagcgccg aacgatccgc aaatgcacta caaagaaaaa tgccagcagg aaggtattcc
    29041 ggttccggca ggaatattcc attatctggc agagaattaa tcactatttt ttattataac
    29101 gatagaaaag cgctgtattt atcgtttctg ttatattaac caggagcagg atatgaaagc
    29161 acttcgtcct ctgacggcat cgcttatgct gttaaccagt tgtttattaa tatctaatac
    29221 cacactggca aaaacaacat taaaactgag tcataatcag gataaaagcc atgctgtcca
    29281 taaagcatta agctatttag cggataaaac caaagagtat tctaatggtg aattagttat
    29341 tcgcatttac ccaaatgcga cattaggtaa tgagcgtgaa tcactggaat taatgaactc
    29401 cggtgcatta cagatggtta aggttaatgc cgcatcacta gaatcatttg ctccggatta
    29461 cagccttttc agtctgccgt tcttattccg cgaccgtgat cactattatc gtgttctgca
    29521 aagtgattta ggtaaaaaaa tacttaattc atcagaaagc aaaggttttg ttggtattac
    29581 gtactatgac ggcggtgcga gaagttttta ttccaataaa ccgattacaa aacctgaaga
    29641 tttagcggga atgaaaatca gggttcaaca aagccccagc gccattgcaa tgatgaaagc
    29701 actcggtggt gtcgctaccc cgatggcgca aggcgaactc tacaccgcac ttcagcaagg
    29761 agtggttgat ggcggagaga acaataccgt tgtctatgcc gatatgcgtc acgccgaagt
    29821 cgcaaaagtc tattcacgtg atgaacacac catggtacct gatgtgttaa ttatcagcac
    29881 cgacgtgttg aataaacttg gtgataaaga gcgcacggca ttattaaaag cagccgatga
    29941 gtcaatgatg cagatgaagg acgtcatttg gcctgcggct gaaaaagaag cctacgacaa
    30001 aatgaaaggg atgaacgcaa cagttgttga tgttgataaa tccgctttca aagaacgcgt
    30061 caaaccgtta tacgatgaat tcaaggcgaa agatgcacaa tcagccaaaa accttgagct
    30121 agtagaaagt atgtaaccct aatgggcagg taattcctgc cttttcatgg aagacttatg
    30181 cgaacactca ctcatattct taataagatc cttgccggat gttgctgcat tattctcgcc
    30241 attatggtgt tctgcgtaac ctggcaagtt attgcacgtt ttatttttaa cgctcccagc
    30301 actgtgcttg atgagttcac ccaaatttta tttatgtgga tgattttact tggcggtgta
    30361 tatactgcgg ggttaaaaaa acatctggcc attgatttgt tggctcaaaa acttcctgct
    30421 gcatctgttc tgacgcttga cagttttatc caaattatca ttacggtctt tgcggttatt
    30481 ttcatgatct atggcgggaa tattgttgtt gaaaaagcac aacatgttgg acaaatctct
    30541 ccagtattaa aatggccaat ggataaagta tattgggtta tgcctgtcag tgggcttatt
    30601 ctggtctggt attcagtaat gaatattatt gacaactatc gtaaacggaa ttctcattaa
    30661 cgagggaaat atggaatttg aatatatcta ccccgtctta attttatttg gcagttttgc
    30721 cgtcatgctg gcaataggtg tgccaattac ttttgcgatt ggtctttctt cgctgttatc
    30781 tattattact gccttgccac ccgacgctgc catttctgtt atttcacaaa aaatgactgt
    30841 agggctggat ggctttacgc tattagccat tcccttcttc gtgttagccg gaaacattat
    30901 gaataccggt ggtatagcca gacgactggt taacctggcg caagcattag ttgggcgtct
    30961 tcctggctca ctggctcatt gtaatatcct cgcgaataca ctgtttggtg cgatttcagg
    31021 ttcagccgtt gcgtcagccg cagcggtagg tgggattatg tcaccactgc aagaaaaaga
    31081 gggatatgat ccggcgtttt cagcagcggt taatattgcc tctgccccca ttggcctgat
    31141 gatcccaccg agcaatgtgt tgattgttta ttccctcgcc agtggcggga cttctgtcgc
    31201 tgcgttgttc ttagctggat atctgccggg catcctcacc gctgttgcct taatgtttgt
    31261 agctgcactt tatgcccgac gtcaccatta tccggtggcc gagcgcatta attaccgtca
    31321 gtttttacag gtattccgcg aatcaattcc tagcctgatg cttattttta tcattattgg
    31381 cggtattatc gccggggtat ttacgcctac agaagcatcg gcaattgcgg taatttatag
    31441 tttagccctg gcgatgattt accgggaaat cactcttaag aagctcaatg atattctgtt
    31501 agattcggta gtaaccagtt caattgttct gttactggta ggctgctcga tggggatgtc
    31561 atgggccatg acgaatgctg atgttcctga gttgatcaac gaactgatta cccgcgtttc
    31621 ggataacaaa tgggtcattc tgtttattat caatatcatt ctgttgatcg tcggtacctt
    31681 tatggatatc acgccggcga tcctgatatt tacacctatt ttcctgccga tcgcccagca
    31741 tctggggatt gatccgattc acttcggcat cattatggtt ttcaacttaa ccattggcct
    31801 ttgcacacca cctgtcggca ccattctgtt tgtggggtgc agcattggta aggtcagtat
    31861 cgacagggca ataaaaccat tactgccgat gtttctggca ttgtttgtag taatggcaat
    31921 tatttgttat ttcccgcagc ttagtctgat gttgccagga ttattttcga cctgatacac
    31981 aatgaagact gcatactgag taattttcaa tgccccacca ctctgtgggg cattgaattg
    32041 gtccattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    32101 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    32161 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    32221 ggcatcgcgc cattcacgtt acggatctgg aacatcacgc cttcaatatg gaacgcttgc
    32281 ggctcgtccg cgcgcaccgt ccagcgttcc cacgttcctt gctgcgcagt aacatcaata
    32341 cggttgacgt cccacaactg cccattaatc cccgggtcat cacccagact gatatcacga
    32401 cttcgaatcg gcgaaccggc catgatttca gttggcagca agcgcatcgg aagactgtca
    32461 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccagagtaga aaccagaatg
    32521 ctggatggct caaagaagcc acgaatacga tcaacaatgc tcgccgcttc gccacaggta
    32581 atcgacactt catcaccgtt gctcatatcc accagaatct cacgacgctc gcccggtgcc
    32641 agcgaaagtt gcttcaccga tacaggagcg ggcaggaatc cctgatcgcc agaaatcaca
    32701 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcaac
    32761 agacgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    32821 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    32881 ttatccagcc gtttatcctg aataatgacc ggaaaatcat ccacaccata atggttgggg
    32941 ataggcagcg atttactgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    33001 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    33061 cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    33121 atcagcgggc caggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    33181 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    33241 cgaccattga ttccccaaac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    33301 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gcagcggcgg aacgggtagc
    33361 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    33421 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    33481 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    33541 ttccggctgc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    33601 tcgaacgaca atgggcagcc aactcgcgaa cctgatcttt gccatactga ctgacgtcaa
    33661 ttggcggcag catttcgaca atcaccagac cgttgtgtag ccgattaaga ttaatcttat
    33721 tcgaagttgt agagacgcac acgggtataa tcgggacgcc cgccgcaatt gccgcgtgaa
    33781 atgccccggt cttgaacggc agcaggccgc gaccacggct gcgggttcct tccgggaaca
    33841 tccagatgga aatacggcgt tttttgaagt gattcactac ttccgcaata gtgccgtgag
    33901 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    33961 agaaggggat ccacagcaag ctctttttac ctaccgtcac tgtcggcggc tgcacaatgt
    34021 tcgatgccgt caccatgtca taattgttct ggtggttagc gatatagata gcattgccgt
    34081 agctttcagc gtctgcaggt ttgcggcact ccactttcag gccgaaaagc ggcgcaagac
    34141 gaccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    34201 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    34261 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaaatc
    34321 aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc gcaacgcatg
    34381 aataaacaac ggtttcaagg gaatttttat cgtttccggg gattgaatac gctgccggat
    34441 gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacagcg ccctccccgg
    34501 catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc tcaacacgat
    34561 cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca gtgactttct
    34621 gtagctcttc cggacgcagt ttaattttgc gtttcccaac atgaatggtc agcgtgcttt
    34681 gcggcggcag aacgtacagt tgcgccaggc catcctcgcc acgcgcggct tctgccgatg
    34741 gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg cttaccggga
    34801 acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca tcttcaatca
    34861 ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcctta cctgcacggt
    34921 tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg gaagccatca
    34981 gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgctcca ggcggcaacg
    35041 ttaatttgcc ggtgagcggc tcgccctgac cacgcgccga cggcagcgta atcgggtcga
    35101 tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc ttacctttca
    35161 ccgccgcttt gaagctatca cccgctttat aattcaggcc cggcgcgtcg atatcatggc
    35221 ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt tcagacggca
    35281 gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc atgcaacggc gaacgacgtt
    35341 cgtcaccgta gccttgcgcg tctgcctgca gttctttctt cagcaggtta ttcattttac
    35401 gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagctca ctctgctcac
    35461 cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg aggatcgctt
    35521 ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt tcatcttcat
    35581 tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc caccagcaaa ccttcgagga
    35641 tatgcaggcg cttgagaact ttctccagac gatagttcag tcggcggcgc acggtatcgc
    35701 ggcggaacac cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat
    35761 ccagaccgat catattgagg ttaatgcgat agctcttttc cagatcggtg gtagcaaaga
    35821 ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc accagacggg
    35881 tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc
    35941 gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggcaatgcgc
    36001 tgataaccac cgcgccatct tccttcttcc aaaccgcgcg catacgcact gaaccacgtc
    36061 cgttctcgta gattttacga atctcggcgc gcgaagtgat aatttccgct tcagtcggat
    36121 aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc tggtcgatta
    36181 aggcaattac cgcctgagcc acttcacgca ggttatgcgg tggaatatcg gtcgccatgc
    36241 cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgggca ggcagcattt
    36301 tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct
    36361 cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga
    36421 acgatttcgg atcgtccggc gccccccagt tcccctgacc atcaaccagc ggataacggt
    36481 aagagaacgg ctgcgccatc aggaccatcg cttcataaca ggcactatcg ccgtgcggat
    36541 ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat
    36601 tcaggcccag ctcagacatc gcatacacaa tgcggcgctg aacaggtttc agaccatcac
    36661 caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt
    36721 ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa
    36781 cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc
    36841 gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccggcggc gataaaggca
    36901 tcaatgtgcc ttgatataca actctttgct gtagacataa tcctggggat tatgcagcgg
    36961 aaaaccgcca acgtagggtt taagcagttt gattaacggc tgatagtaga tgggaatcag
    37021 cggtgcctgc tggttgatga tcacttctgc ctgctgatac agcgcattac gctttgttgc
    37081 atcagccgtt tgtgccgcct ggtttagtaa ggcgtcatac tgcgcgtttt tccagtggcc
    37141 gacgttttct tcactatcgc ttttgagcgt gttcaggaag ctggaggcat cattgtacgt
    37201 cgcatcccac gactgccgag acagcataaa atcaccggct cgtcgggcat caagataggt
    37261 tttccactcc attgtgcgca gcgtcacctg tgcacccagc cattttttcc attcggaaga
    37321 caacgctatc gcggtctttt catgcagatc gtacttgttg tagaacagct caaagcgtag
    37381 cggatgagag gcgtcgtatc ccgcctgttt cagcaagact tttgccatcg cgacgcgctc
    37441 actcattggc ttttgcagtt catcgaacgt cgtcgcgcta aagcctttta cctctggcgg
    37501 cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt tgcgcaataa gctgtcgatc
    37561 aaccgtaaga tatagcgccc gacgcacccg cacatcgtta aatggcggtt tctcaaggtt
    37621 aaagttgtaa tattcgctgt tcagacgcgg gataattcgt agctcgccag gcagtgattt
    37681 ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga tcgacctctc ccgcgcgata
    37741 gcggttatag ccggtgaccg aattatccag cgccagatac tcaacctgtt gcaatactgt
    37801 atgttgcgca tcgcggtact ttggattttt gcgtgcagta atcttttcgt taactaccca
    37861 tttatcaagc acaaaggcac cgttgtaaac catgttctct ggcttactcc agctatcgcc
    37921 atgtttagcg atgacatgat gaggaaccgg gaacagcgtc ggccaggcga gcatcgtcgt
    37981 gaaccaagga accggctgct caagcgtaac ttcaagagta cgatcatcca tcgctttgac
    38041 acccagcgat gtaacatctg ctttacccgc aacaatagcc gcggcattgt taatgtgcgc
    38101 ctgtgccaga tagccagcaa aagggcttgc cgttttcggg tcaaccgcgc gctgccagcc
    38161 gaggacaaaa tcctctgccg tcagaggctg accgtctgac cactgcaaac cgctacgcag
    38221 atggaaaata tagcgcttgc cgccgtcaag tatctcccag cgttcagcct gagcgggctg
    38281 cacctggcct tcaccgtcca tccataccag accttcaaac agatccagca caatctgcgc
    38341 ggcggtattc tcctctacct tttgcgggtc gagcgtgcct gggtcgctat gattgttgta
    38401 acgaaagact tgttgcgggg cgagcggtgt gttggcggga acgtcagcag cgtagagagg
    38461 agccgcactt accaggctga ccagccataa taaatttcgc gtatacatgg tattccttac
    38521 cggagaaatt ataagcccga caacaatacg cctgtggcat tgtttgctca aacttatcgc
    38581 gagtgatttg gctcacactc cggtaaagat tccccatcac gctaacggta cataaacatc
    38641 catttccagt cgctgctctg tcatcccttc ggcaagattg gtgtagtgaa acagaatcgg
    38701 tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaatgatgc cccatatcgt
    38761 gtgtgaaata tcgtccagct cgccaacatg gcgggccaca gcataacgtc caccggtaag
    38821 ttcaccatta cttacgccat aacgattatc ggggatcggt tcgctaacgc tgccgcagat
    38881 atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg tcccaggcga cgccaaaagt
    38941 ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa atttcgccac
    39001 gctgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag ggattgatgg
    39061 gaaatcgata atatttacat ccagtgtcag gtttgtcatt ttgcctccct gcgaaattat
    39121 tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg atctgtttga
    39181 cgtcaatttc aacagagttc cagtctttat cgacttcacc ctgaatctca accgtatctt
    39241 tcggcgtcac cgtcacgccg ttccagcgtt tatggtcgat atcaacattg atagtaccgc
    39301 tggcatcttt gaacacgtag agatcgtcag agatgcgttc aacgatattg ccgcgtaggg
    39361 ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg ctgccgttcg
    39421 gcccctggaa tcctccggcc tgactttgcg ttgccgatgg gccagaaaaa cctccctgct
    39481 ctgctgccat aaccggtgca ctgcacaggg ccattactgc gattactgct gcgaattttt
    39541 tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata gtccttaaca
    39601 acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca acacggttta
    39661 ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg atagaagatg
    39721 acatgctgat tggcgacggc atcaaaacgg gccttagtaa aatgggtttt agcgtcgact
    39781 ggtttacaca aggtcgtcag ggaaaagagg cgctatatag cgcaccttat gatgcggtga
    39841 tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa tggcgagaaa
    39901 aaggtcagcg tgagcctgta ttgatcctga ctgcgcgcga tgcgctggcg gaacgtgtag
    39961 aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg atagaagtcg
    40021 ccgccaggct ggaagctctg atgcgccgaa ccaacggcca ggccagcaac gagctgcgcc
    40081 acggcaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc gaacccttaa
    40141 cgctgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct ggtcgggtac
    40201 tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc accagtaatg
    40261 ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt attcgtaccg
    40321 tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc ttagtctgcg
    40381 agtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc tttccagctt
    40441 tgtcgcctgg aaacaaacaa cggataacgt cgatgagttg ttcgacaccc aactgatgct
    40501 gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg atcgcatggc
    40561 acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga cttttgccat
    40621 ctttacccac gacggcagaa tggtccttaa cgatggcgat aacggagaag atattcccta
    40681 tagctatcag cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg atccctggcg
    40741 ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgtgggtc aggaatggga
    40801 ataccgtgaa gacatggcgc tggcgattgt tgccggacaa ttgatcccgt ggctggtcgc
    40861 actgccgatt atgttaatca tcatgatggt gctactgggt cgtgaactcg cgccgctgaa
    40921 caaactggcg ctggcactac gtatgcgtga cccagactcg gaaaaaccac taaacgcgac
    40981 gggcgtaccc agcgaagtgc gcccactggt tgagtcgcta aatcaactgt tcgcccgcac
    41041 acatgcgatg atggttcgtg aacgacgctt tacctccgac gcagctcacg aacttcgtag
    41101 cccgttaacg gcgctgaaag tgcaaaccga agttgctcag ctctctgacg atgatccgca
    41161 ggcgcggaaa aaagcgctgc tccaattaca ttccgggatc gatcgcgcta ctcgtctggt
    41221 tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg acgtcgcgga
    41281 gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat atttaccaca cggcgcagca
    41341 ggcgaacatt gacgtgcgac tgacactcaa tgccaacggc atcaaacgca ccggacaacc
    41401 gctattgcta agtttgttgg tgcgaaattt gctggataac gccgtgcgtt acagtccaca
    41461 aggtagcgtg gtagacgtca cgctgaatgc cgataatttc atcgtgaggg ataacggccc
    41521 tggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc cacccggaca
    41581 aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca aattgcatgg
    41641 catgaatgtt gattttggga atgcggaaca aggtggattt gaggcgaagg taagctggta
    41701 acttctctgt ctatgagagc cgttaaaacg actctcatag actttactaa tagcaaaata
    41761 taaaccgtcc ccaaaaaagc caccaaccac aacccagaca aataacgagc gagaatacat
    41821 atagccaaaa ctcgaacatt tcccgcataa agagttttct taagataaga ataataagtg
    41881 gtgtcagaag aaaaaatgct gcaatcattg cttgtaatat acgatcttcg cctgaaacag
    41941 aaggtatact gcaaatattg tctcccgact caagcatcca gtcatactta ttgaaagcaa
    42001 a
//
LOCUS       DAZFRE010000030        41031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFRE010000030 DAZFRE010000000
VERSION     DAZFRE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.030"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(737..1774)
                     /gene="rhuM"
                     /locus_tag="ACHKUI_001983"
     CDS             complement(737..1774)
                     /gene="rhuM"
                     /locus_tag="ACHKUI_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFO6598179.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     NQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            complement(1827..1921)
                     /locus_tag="ACHKUI_001984"
     tRNA            complement(1827..1921)
                     /locus_tag="ACHKUI_001984"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1885..1887),aa:Sec,seq:tca)
     gene            2214..3596
                     /gene="yicJ"
                     /locus_tag="ACHKUI_001985"
     CDS             2214..3596
                     /gene="yicJ"
                     /locus_tag="ACHKUI_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6598180.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3606..5924
                     /gene="yicI"
                     /locus_tag="ACHKUI_001986"
     CDS             3606..5924
                     /gene="yicI"
                     /locus_tag="ACHKUI_001986"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6598181.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWAFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIWQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            6219..7772
                     /locus_tag="ACHKUI_001987"
     CDS             6219..7772
                     /locus_tag="ACHKUI_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO6598182.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LKQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            7799..8269
                     /locus_tag="ACHKUI_001988"
     CDS             7799..8269
                     /locus_tag="ACHKUI_001988"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO6598183.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8287..8601
                     /locus_tag="ACHKUI_001989"
     CDS             8287..8601
                     /locus_tag="ACHKUI_001989"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6598184.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8626..9717
                     /locus_tag="ACHKUI_001990"
     CDS             8626..9717
                     /locus_tag="ACHKUI_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6598185.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            9729..10580
                     /locus_tag="ACHKUI_001991"
     CDS             9729..10580
                     /locus_tag="ACHKUI_001991"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6598186.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            10661..11521
                     /locus_tag="ACHKUI_001992"
     CDS             10661..11521
                     /locus_tag="ACHKUI_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6598187.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            11569..12501
                     /locus_tag="ACHKUI_001993"
     CDS             11569..12501
                     /locus_tag="ACHKUI_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6598188.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            12482..13162
                     /locus_tag="ACHKUI_001994"
     CDS             12482..13162
                     /locus_tag="ACHKUI_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO6598189.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(13206..14915)
                     /gene="yicH"
                     /locus_tag="ACHKUI_001995"
     CDS             complement(13206..14915)
                     /gene="yicH"
                     /locus_tag="ACHKUI_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6598190.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVVDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15036..16427)
                     /gene="xanP"
                     /locus_tag="ACHKUI_001996"
     CDS             complement(15036..16427)
                     /gene="xanP"
                     /locus_tag="ACHKUI_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6598191.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16707..17912
                     /gene="gltS"
                     /locus_tag="ACHKUI_001997"
     CDS             16707..17912
                     /gene="gltS"
                     /locus_tag="ACHKUI_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6598192.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(17946..20027)
                     /gene="recG"
                     /locus_tag="ACHKUI_001998"
     CDS             complement(17946..20027)
                     /gene="recG"
                     /locus_tag="ACHKUI_001998"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6598193.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20033..20722)
                     /gene="trmH"
                     /locus_tag="ACHKUI_001999"
     CDS             complement(20033..20722)
                     /gene="trmH"
                     /locus_tag="ACHKUI_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6598194.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTISDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(20729..22837)
                     /gene="spoT"
                     /locus_tag="ACHKUI_002000"
     CDS             complement(20729..22837)
                     /gene="spoT"
                     /locus_tag="ACHKUI_002000"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6598195.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(22856..23131)
                     /gene="rpoZ"
                     /locus_tag="ACHKUI_002001"
     CDS             complement(22856..23131)
                     /gene="rpoZ"
                     /locus_tag="ACHKUI_002001"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6598196.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23186..23809)
                     /gene="gmk"
                     /locus_tag="ACHKUI_002002"
     CDS             complement(23186..23809)
                     /gene="gmk"
                     /locus_tag="ACHKUI_002002"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6598197.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24067..25749
                     /gene="ligB"
                     /locus_tag="ACHKUI_002003"
                     /pseudo
     CDS             24067..25749
                     /gene="ligB"
                     /locus_tag="ACHKUI_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            complement(25746..26363)
                     /gene="yicG"
                     /locus_tag="ACHKUI_002004"
     CDS             complement(25746..26363)
                     /gene="yicG"
                     /locus_tag="ACHKUI_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6598198.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26655..27479)
                     /gene="dinD"
                     /locus_tag="ACHKUI_002005"
     CDS             complement(26655..27479)
                     /gene="dinD"
                     /locus_tag="ACHKUI_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6598199.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(27701..28564)
                     /gene="yicC"
                     /locus_tag="ACHKUI_002006"
     CDS             complement(27701..28564)
                     /gene="yicC"
                     /locus_tag="ACHKUI_002006"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6598200.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLKAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            28691..29407
                     /gene="rph"
                     /locus_tag="ACHKUI_002007"
     CDS             28691..29407
                     /gene="rph"
                     /locus_tag="ACHKUI_002007"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6598201.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            29473..30114
                     /gene="pyrE"
                     /locus_tag="ACHKUI_002008"
     CDS             29473..30114
                     /gene="pyrE"
                     /locus_tag="ACHKUI_002008"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6598202.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(30151..30747)
                     /gene="slmA"
                     /locus_tag="ACHKUI_002009"
     CDS             complement(30151..30747)
                     /gene="slmA"
                     /locus_tag="ACHKUI_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6598203.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(30854..31312)
                     /gene="dut"
                     /locus_tag="ACHKUI_002010"
     CDS             complement(30854..31312)
                     /gene="dut"
                     /locus_tag="ACHKUI_002010"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6598204.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(31290..32510)
                     /gene="coaBC"
                     /locus_tag="ACHKUI_002011"
     CDS             complement(31290..32510)
                     /gene="coaBC"
                     /locus_tag="ACHKUI_002011"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6598205.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKALLGQLL
                     LDEIVTRYDEKNRR"
     gene            32682..33350
                     /gene="radC"
                     /locus_tag="ACHKUI_002012"
     CDS             32682..33350
                     /gene="radC"
                     /locus_tag="ACHKUI_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6598206.1"
                     /translation="MKNNAQLLMPREKMLKFGISSLTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            33567..33803
                     /gene="rpmB"
                     /locus_tag="ACHKUI_002013"
     CDS             33567..33803
                     /gene="rpmB"
                     /locus_tag="ACHKUI_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6598207.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            33824..33991
                     /gene="rpmG"
                     /locus_tag="ACHKUI_002014"
     CDS             33824..33991
                     /gene="rpmG"
                     /locus_tag="ACHKUI_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6598208.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            34089..34898
                     /gene="mutM"
                     /locus_tag="ACHKUI_002015"
     CDS             34089..34898
                     /gene="mutM"
                     /locus_tag="ACHKUI_002015"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6598209.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(34937..35416)
                     /gene="coaD"
                     /locus_tag="ACHKUI_002016"
     CDS             complement(34937..35416)
                     /gene="coaD"
                     /locus_tag="ACHKUI_002016"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6598210.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(35424..36701)
                     /gene="waaA"
                     /locus_tag="ACHKUI_002017"
     CDS             complement(35424..36701)
                     /gene="waaA"
                     /locus_tag="ACHKUI_002017"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6598211.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            37114..38172
                     /gene="rfaQ"
                     /locus_tag="ACHKUI_002018"
     CDS             37114..38172
                     /gene="rfaQ"
                     /locus_tag="ACHKUI_002018"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6598212.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            38169..39293
                     /gene="waaG"
                     /locus_tag="ACHKUI_002019"
     CDS             38169..39293
                     /gene="waaG"
                     /locus_tag="ACHKUI_002019"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6598213.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            39286..40083
                     /gene="rfaP"
                     /locus_tag="ACHKUI_002020"
     CDS             39286..40083
                     /gene="rfaP"
                     /locus_tag="ACHKUI_002020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6598214.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFSAPLKDILKQEQGLLSQAEEKATKIRERTIRKSL"
     gene            40099..>41031
                     /gene="waaO"
                     /locus_tag="ACHKUI_002021"
                     /gene_synonym="rfaI"
     CDS             40099..>41031
                     /gene="waaO"
                     /locus_tag="ACHKUI_002021"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO6598215.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDQNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPDADIQRLSQLAKSYHTCIQIHLVNCERLKVLP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGTLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFICPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPMNSNYARYC"
BASE COUNT         9877 a         9947 c        10827 g        10380 t
ORIGIN      
        1 agattgaata tattggtagc gcaagtttga gttgctagcc aggatgccgc tggatgactc
       61 aaattaatat gcgtgctaaa gactttgacg cgactacttc tgtttttctc tttactttca
      121 attaaatgcc tagcgctttt ggctgcttta caagttccat gccccacacg gcaaaaataa
      181 tctcctgaaa ggatacttac aatcagtatt acaccaatga cgacagaaaa aatagttccg
      241 atgagtttga tgatgacaat ccttgtgcag tgttattttc accatggatt ataatgttat
      301 agtgaaatta ttgcgcatgt aatttttctt tgattgtttt aacatcacat ttatgcactt
      361 cttcatcttt gtagcaggta aaagccatat atggatggct ggtgtccggt gcagataaaa
      421 tgcaacttat tcttgatagc ttgaaaaaaa gagaggtttt tttgagtgac ggttgatagt
      481 cggtggcaag gaagacgttt tcgtacactc ggcaagaaaa acgaatcact ctgtcatact
      541 accagataca tcagcagggt caggttgctc agaaataagc aggatgggga tcactgaaga
      601 ggcattttgg cgatcacagg agtcgcattt gatggttaag ttattgattt aatgtttata
      661 ttttgaattt gataaatgag taaccccata agtaccccca aatggttttg cgtatggcgt
      721 ttggggggat tccgggctac tttttcgact ctttattcca ctgtagcaaa ccagcgatat
      781 ccttctcgcc ttcggcttct tttaagcgcc gttgttgttc ggcaaactga acatactccg
      841 cctgcgcttt ttcatcggcc attttcttgc tgattttacc agcgccctgt agaacctctc
      901 ggtcgttaaa ttgcaggaac tgatccagct tatcctgcca gtcgcgcaaa aagacttgct
      961 gacgacgacg ggcctgatcc tcggcaaagt ccagccacat attaaccacg cgattaagtt
     1021 cgctgacctc atcctgggtg agataatttt ttgccaccgt cacgtcgccc ttacgtacct
     1081 cttcaccttt ataactggtc aagcccatgt gcggctggtt ggcatctgct cgtttgtgga
     1141 tgagttccgc agcggtatgc ccggtacagg caaaatgcag tttattctgg atggtttgaa
     1201 aaaattgcgt ggtttcttta agcgatggct gatagtcggc agctaaagca aagatctccc
     1261 gcacccgcaa atatacccga cgctcgctgg cgcgaatatc gcggatgcgt tcaagcatct
     1321 catcaaaata gtcgggcaca accgatgaac cgacaggcgg attcttcagc cgctcatcgt
     1381 ccatcacaaa acctttgatc aggtattcct gaagcgtctg ggtggcccac tggcggaatt
     1441 gcgtgcctcg aggcgagcga acgcgatagc cgacagcgag aatggcttcg aggttgtaat
     1501 ggtctatctc tctggaaacc tgacgttttc cttcctggcg aactatccgg aatttccgga
     1561 tggttgagtt ttgaacaagt tcaccttcgg aaaatatatt gattaaatgt tcgttaatag
     1621 tgcgaacatc tttatcgtac agttcagcca ttgccgcctg ggataaccag attgtgtctg
     1681 actcaaagcg acattcaaca cgcactcttc cgtcaccgct ggcaaacata ataaactcgc
     1741 ctgctggggg ctgggttaag tctttgtctg acatgtcgcc tccgaaggag tgttgttgac
     1801 gtcgggagag ttcaggagag gcattttggc ggaagatcac aggagtcgaa cctgcccggg
     1861 accgctggcg gccccaactg gatttgaagt ccagccgcct caccggagac gacgatcttc
     1921 cgcgcctcga ttgctacatg gaggcggggc gcattatagc tacttccttg agtttctaca
     1981 tcccccagat cagttttttc cgcctaatac acgcagcgta ttatcacgaa aacattatag
     2041 ttcatataaa actcatattt tacaatgata tatgagcgaa attaacgcta ccacgatcac
     2101 ataaaacaag aatcataaat taataaccag atatcggaat attcgctctc gcagggatgg
     2161 tttacaaaat gcgttatcgg tctcagcacc cctatagcat caaggaaaag cagatgaaga
     2221 gtgaagtgtt gtccgttaaa gagaaaattg gttatggcat gggagacgcc gccagccaca
     2281 ttattttcga taacgtaatg ttatatatga tgttctttta taccgatatt tttggcattc
     2341 ctgccggatt tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg atttccgatc
     2401 cttgcatggg gttgttggcc gatcgaacgc gctctcgctg gggtaaattt cggccgtggg
     2461 tgctgtttgg cgcactgcca ttcgggatcg tctgtgtact ggcctatagc acgccagatc
     2521 tcagtatgaa cggaaaaatg atttatgcag caattactta caccctactt accttacttt
     2581 ataccatcgt caatatccct tactgcgcat tgggtggtgt aatcaccaat gacccgactc
     2641 agcgtatctc gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc atgctctcta
     2701 ctgttctgat gatgccactg gttaatttaa ttggtggtga taataaacct ctcggtttcc
     2761 agggcggtat cgcggtcctt tccgtggtgg catttatgat gctggcattc tgtttcttca
     2821 ccactaaaga acgcgttgaa gcaccaccta caacgacgtc tatgcgggat gatttacgtg
     2881 atatctggca aaacgaccag tggcgtattg tcggtttcct gaccattttc aatatcctgg
     2941 cggtgtgcgt acgcggtggg gcgatgatgt attacgtcac atggattttg ggcacgccgg
     3001 aagtatttgt cgcttttctc accacttatt gcgtgggtaa cctgattggt tccgcactgg
     3061 cgaaaccgct gaccgactgg aaatgtaaag tcactatctt ctggtggacg aacgccctgc
     3121 tggcagtgat tagcctcgcg atgttctttg ttcctatgca ggccagcatc accatgtttg
     3181 tcttcatctt cgtgattggt gtgttgcatc aactggtgac acctatccag tgggtaatga
     3241 tgtccgatac cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc gggatcagtt
     3301 ttgctggcac gctgtttgtg ctcaaactgg ggttggcctt cggtggtgcc cttatcggct
     3361 ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc gtcacaatta
     3421 gcatcattat cgcgctattc acgattgttc cggcgatctg ttacttgctg agcgcgatta
     3481 tcgctaaacg ctactactcg ctcacgacgc acaatctgaa aaccgttatg gaacagctgg
     3541 cgcagggcaa acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa
     3601 cggcaatgaa aattagcgac ggaaactggt tgattcaacc tggcctcaat ttgattcacc
     3661 cgcttcaggt gttcgaggtt gaacagcagg gtaatgaaat ggtggtctat gctgcccctc
     3721 gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg cgctttttct
     3781 ccccacagga aggtattgtc ggtgtacgga ttgagcattt tcagggggcg ctgaataacg
     3841 gtcctcatta cccgctcaat attttgcagg acgtgaaggt cactatcgaa aacacagaac
     3901 gttacgctga gtttaaaagt ggcaacttaa gtgcgcgtgt cagcaaaggt gagttctggt
     3961 cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataacggct
     4021 acgtgcagga cacgaataat caacgaaatt atatgtttga gcggctggat cttggcgttg
     4081 gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat ggtcagacgg
     4141 tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt
     4201 tttatatgac taaccgtggt tatggggtac tggtcaatca tcctcaatgc gtctcttttg
     4261 aagtgggatc ggagaaagtc tccaaagtac agttcagcgt tgagagtgaa tatctcgaat
     4321 actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc cgttttactg
     4381 gccgtccggc gctgccgccc gcgtgggcct tcggtctgtg gctaaccact tcatttacca
     4441 ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc
     4501 cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg
     4561 agtgggaccc gctgactttt ccggacccgg aagggatgat ccgccgtctg aaagcgaaag
     4621 ggctgaaaat ctgcgtctgg attaacccct atatttggca aaaatccccc gtctttaaag
     4681 agttacaaga gaaaggttat ttactcaaac gcccggacgg ttcgctgtgg cagtgggata
     4741 aatggcagcc aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg
     4801 ccgacaaact gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg
     4861 aacgtattcc aaccgatgtt cagtggtttg atggttccga tccgcagaaa atgcataacc
     4921 attatgcgta catctacaac gaactggtgt ggaacgtgct caaggacacc gttggtgagg
     4981 aagaagccgt cttgtttgcc cgctcggcct ccgttggtgc gcagaaattt ccggtacact
     5041 ggggtggcga ctgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt
     5101 ctattggcct ttcaggtttt ggcttctgga gccacgatat cggcggcttt gaaaataccg
     5161 ctccggcgca cgtttacaaa cgctggtgcg cgtttggttt gctttccagc catagccgtt
     5221 tacatggcag caaatcttat cgtgtgccgt gggcctacga tgatgagtcc tgtgatgtgg
     5281 tgcgcttctt cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagctgcac
     5341 gtgcaaacgc ccggggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc
     5401 cggcttgtga ttaccttgac cgtcaataca tgttaggcga caacgtgatg gttgcgcctg
     5461 tgttcactga agcgggcgat gtgcagttct acctgccgga aggtcgctgg acacacctgt
     5521 ggcacaacga tgaactcgac ggtagtcgct ggcataaaca gcagcacagc ttcctgagtc
     5581 tgcccgttta tgtgcgtgat agcaccctac tggcgctggg caacaacgat caacgtcccg
     5641 attacgcgtg gcacgaaggt acggcattcc acctctttaa tctgcaagac gggcatgagg
     5701 ctgtctgtga agtgcccact gctgacggtt cggtgatctt tactttaaaa gcagcacgta
     5761 ctggcaacac gattactgtg actggtacgg gcgaggcgaa gaactggaca ctgtgcttgc
     5821 gcaatgttgt gaaagtaaat ggtctgcaag gcggttcgca ggctgaaagt gagcagggac
     5881 tggtggtgac tcctcaaggg aatgcgctga caattacgtt gtaatctatt gaagaatgcc
     5941 ggatacgaat ggttttgtgt cgtatccggt aaataatctc acatctggta ataaatattt
     6001 cttccatcgt atttgcgagc atgatcacga ttattcatca cgaaaatgtg atctgtgctg
     6061 aaaaatatcc cttaaccgcc ttgctttgtt ttatcactgt ccggattacg gacagatttt
     6121 agtattgttg tgaaattatt ctgctttata tttaccctca gagatggcaa ctgaagctct
     6181 tccatatggg gcttcttaat ctgtgataag ggtaaaaaat gcaaatgatt acctcgcgac
     6241 aaaacagatt attacgattc cttttacctc gaagggaata tacgactatt gttacgattg
     6301 ccggctattt aaatgtttcg gaaaaaacca ttcaacgtga tttacgttta cttaagcaat
     6361 ggctgggaca atggagaata aatgttgaga agcgtgctgg cgcgggtgtg atgttaagcg
     6421 cggagaatat tgctgatttg ctgcatcttg atcatttgtt ggtggcagaa tgtgaagaga
     6481 ttgatggtgt aatgaataat gccaggcgcg ttaaaatagc gtcgcagtta ttaagtgaaa
     6541 caccgaatga aacgtcgatc agtaaattgt cagaacgcta ctttatcagc ggagcctcta
     6601 ttgttaatga cctgagagta attgagtcct ggcttgcgcc gttggggtta tcgttgatcc
     6661 gcagtccaag tggtacgcat attgaaggta gtgaagggca agtccgacag gcaatggcat
     6721 tactgattaa cggcattatt aaccataatg agccgcaagg tgtcgtgtat tcacgtctgg
     6781 atcccggaag ctataaagca ttagtccatt attttggaga agaagaggtg ttatttgtcc
     6841 agtcattgtt actggatatg gaaaatgaat taagttggtc tttgggagaa ccttattacg
     6901 ttaacatttt tactcacatc cttattatga tgtatcgcaa cacgcacggg aatgcgttat
     6961 caagagaaga agatcaaacc agacaatatg atgaaaatat ctttaatgtt gccagtcaga
     7021 tgattcataa gatagaacaa cgaattgcac atacattgcc cgatgatgaa gtctggttta
     7081 tttatcaata tatcatttca tcaggtgtgg cgattgatgg acaaaaagat gttagcatta
     7141 tttcacatat gcaggccagc aatgaagcgc gtctgattac ctggcgttta attacggtat
     7201 tcagtgacat cgtggactgc gattttagtg aagacagcgc attatatgat ggcttattgg
     7261 tgcatattaa gccgctgatt aaccgtctaa attatcgcat tcatatccgt aatccattgc
     7321 tggaagatat taaagcagaa ttagcggatg tctggcggtt gacgcaatat gtggtgaatc
     7381 aggtatttaa aacctggggt gagaatgcag tgagcgagga tgaagtgggt tacctgaccg
     7441 ttcattttca ggctgcgatg gagcggcaaa ttgcccgtaa acgtgtatta ctggtctgtt
     7501 caaccggaat cggaacttcg catctactga aaagccgtat tctgcgagca tttcctgaat
     7561 ggacgattgt tgatgttatt tcagcagcga atttatcaca ggttttgcct gacaatatcg
     7621 aactgattat ttcgaccatt aatttgccta cagtcactat gccggtcgct tatgttaccg
     7681 ctttttttaa tgatgccgat attaagcggg tcactgaaat ggtgattacg gaaaaattac
     7741 atcatgcgac gtctcgggtc gttgaaattt aatgtcagaa ttttatgagg tatgacgaat
     7801 ggacataaca aaaatcctga atacgaatcg cgttatttta gatatgcaag cgacgaataa
     7861 agcggaggcg attgaagaat taactaatct tctcaaaaaa gatggtgcga tcgattgtcg
     7921 ggaaaccttt attaaagatg tctggcagcg cgaagcggaa ggctcgactg gttttgaaaa
     7981 tcatattgcg atcccgcatg gtaaatcttc tgcggtaatt aatactacgc tggcaattgg
     8041 ccgtacccgc caggatattc cctgggaaac gctggatggc agtagcgtgc gctgtattat
     8101 tttatttgcg gtacgtcttg aagatcaaaa taccacccat atccgtttac tttcccaggt
     8161 cgcgggcgcg ctggcggatg atgacatcat agaacaatta ttagttgaga cgagtccgca
     8221 aaaaatcatt gatctcttta gtcagtatgc acaatccgat gtctgctaaa taacaggaag
     8281 tatgtcatga atattgtctg tgttgccgcc tgtacggcgg gtattgctca tacctatatt
     8341 gctcgtgaaa agttaatcaa aggagcaaaa gctctgggcc ataccattaa agttgaaacc
     8401 cagggaacca ttggtacaga aaatgaatta caggcggcag atatctctgc tgctgacgtc
     8461 gtgattcttg ctgtagatgt gaaaataaaa ggagaagaac gcttcaccaa taaacgtatt
     8521 gtgcgagtaa agacagagat cgtcattaaa tcgcccgttc aatttcttga aaaggtcgaa
     8581 aaatcactgg gaaacaaatg atccttatac tggagggatc ttaatatgaa agacaataaa
     8641 attccggttt cacaggaaat taaaaaacat ttattaaccg gcatctcgtg gatgataccc
     8701 cttattgtgg ccgccgggat atgtattgcg ttggggcaag ttcttggcgg tacagatgtt
     8761 ggtgaaaaaa caggaacaat cccgtggatg cttaatcaga ttggcggctg gggaatgggg
     8821 ctgattgtgc cgctgattag cgcggcaatt gcctattcta ttgccgatcg tcccggattt
     8881 gccccgggcc tgattgtcgg ctttctctgc gggcaaatcc ataccggatt tattggcggt
     8941 atgttgggtg gtttcctggt gggctacacc attttgctac ttaaacgcta tatccgcctg
     9001 ccgcagtcga tgcaaggact catgccaatc atggtgttgc cggtattaag caccattatc
     9061 ggtgggctgt taatgatgac gctgattggt aaacccatcg cctggttaca agaagcactg
     9121 attcatttgc tcgagtcgat gcagggcgga tcgcgcttcc tgatgggggc catacttggc
     9181 gcgatggcaa ccttcgattt cggtggtccg gtcaacaaaa ccatgtcgct gttttccgat
     9241 ggcttgctgg tcagtggcgt ctacggacca gaagccgtga aatttgtcgg ctcaatcatc
     9301 cctccgtttg gcatcacact ttcgttctta ctgacacgcc ataaatacac ccgtgcggag
     9361 cgcgaggcgc tgaaagccgc gtttccgatg gggatctgca tgatcactga aggagtcatt
     9421 ccgatcgccg cgcgtgacct gctgcgagta gtgggatctt gtgtggtggc atcggcggtc
     9481 gcgggcggct taatcatggt gtggggcgtg gagagtccgg ttccgcatgg cggcatgttt
     9541 gttgtgcctc tgttcaccca tcctctgctg ttttgcctgg cgctgggtat aggcacggtg
     9601 atttgtggcg tgatgctttc actctggaag aaaccggtaa cggaacgtga cgaagagttt
     9661 gacgaactga gcgatcaaaa gctgaaagat gaagagatca ctttcaccct cgaataaccg
     9721 gaggccctat gtttgccgat atgaaaagta tggtgatgaa agcctggcat gaacattacg
     9781 ccttactggc gatcaactgt atgaatctgg aaagtgctca cgcagctatc cgcgtggcgg
     9841 agaagaatcg cgctccgatc attctcaatc tctatcaggg gcatctggcg cattttcctg
     9901 cacccgtcgc ggcggcagtg gttaaaacgc tcgcggaatc tgcctcagta ccggtcgctc
     9961 tggcgcttga tcatggcaaa aatccggact gtattcgtca ggcgtttcgt gctggtttca
    10021 gcggattaat gattgatgct tctgcttttc ccctggagga aaacgtgcgc cagaccaggg
    10081 cggtggtgga actcgccgcc agcgtgcagc tctgcgtgga aggcgaactg ggccatctgg
    10141 cagatgcgcc gcgttacgac caggccgcca atgcagacct gatgacgcaa cccactgatg
    10201 tggaaccgtt tatcgcgcag acaggcattg atctactggc cgtctcggta ggcacggcgc
    10261 acggcatgta cgcgcctggc gttgtacctg ctattgattt tcagcgtctg gcggagattt
    10321 cgcgctgctc gagcgtgcct ctggcattac atggcggtag cggtacgccg ttcgatcagc
    10381 tccagctctg tacgacgctg ggggtagcaa aaatcaacgt gggggcggcc atttttgaac
    10441 gggggaaatc cgcgctcctg cataccctct gtcacgatat atccattgaa ctggtggacg
    10501 cgctgaaagc tatggaactg gccttcgaag aagcgattac accgtattta caggcgagcg
    10561 gcgctatcgg caaagcctga ttcattttct gtcatggcct ttatgccagt gcaataacgc
    10621 accggcaggg cggactacac gctgaaactg gagatttaac atgttagttt cgatgaaaga
    10681 attactgcaa cctacccgcc agcacggctt tgccattggc gcatttaatg tggcggataa
    10741 ctgtttcctt cgcgcagtcg tggatgaagc agaagcgaca aatactccgg cgattattgc
    10801 catccatccc agcgaacatg attttgtggg cgatgccttt ttcgctcacg ttcgcgaaat
    10861 tacccagcgt agtccggtcc cgtttgtact gcacctcgat catggtgcct ctgtagagca
    10921 tgttttgcgt gcgattcgct gcggatttac ctcagtaatg atcgacggtt cgctgctgcc
    10981 gtatgaagaa aacgtggcgc tgaccgcaga agtggttcgc ctcgctcacg cggttggagt
    11041 atcggttgag ggtgaactgg gaaccatcgg acaaacagga acgtcggtgg aaggtggtgt
    11101 ttcacaagtg acctacacag atccggcgca ggccgccgat tttattgctc gtacaggtgc
    11161 cgacacactg gcggtagcga ttggtacggc acacggcatc tatccaaaag ggatgcaacc
    11221 gaagctgcaa atggacattt tgcgcgacat tgccggacgc attgatattc cgctggtact
    11281 acacggtggt tcggcaaacc cggacgcgga gattgctgaa tcagttacgc tcggcgtcgg
    11341 caaaatcaat atctccagcg atatgaaata cgcctatttc cagaaagtgc gtgaaattct
    11401 cgctaaagag agctggtggg acccgaacgt catctatccg gatgcgatta acgcggcgcg
    11461 ggaagtcatt cgccacaaaa tgaaactgtt cggttcgctc ggtaaagcat cactgtacta
    11521 agccagaagg gcggggtgac ccgcctttta cgcaacaagg aacgagatat gcactatctg
    11581 gggttggata ttggtgggac caaaatcgcc gccgttgtca tggatgcgca tggctgggag
    11641 attcgccgtt accgctgccc gacgcaaaag tcgacatatc aacaatttgt ctcatgcgtt
    11701 gtggcgctta tcgagcagat taggcgggac gttcaacgac cgatgctgac agggatcgcc
    11761 ttacccggca gtatctcgcc actcactggc ctgattaaaa acgcgaatat tcaggtgatt
    11821 aacggtcatg cgttacaggc tgatttgcag caattgcttg ggcaaccggt ggtgatagcc
    11881 aatgatggta actgtttcgc gctatcagaa gcttgcgacg gtgccgggca agattatgac
    11941 gtggtatttg gtattacgct tggtacgggc tgcggcggtg gcattgccat caagcaacga
    12001 ccgtttatag gggcctgggg aaatgctgcc gaatgcggtc atatcacgtt gccaggctat
    12061 acggagcagg aagatggtcc atcagtcagt tgctattgcg gcaaacacaa ctgcgtggag
    12121 tcgtttgttt ccggcagcgg ttttagtgaa cgctatcaac agatgactgg taacttgctc
    12181 actcctgcgg cgattgtcac cctggcacaa cgtggtgatg cttgtgccat gcagcaggtg
    12241 gcacgttttc gccaacagct tgcccgcacg ctggcaacca tcgttaacgt tgttgaccct
    12301 ggcgtgattg tgatcggcgg cgggctttcg aatgtggaac tgcttatcac cgatctgaac
    12361 gcagaagtcg ctccactggt tttcaccgat caattcacca ctcccattgt aaaagcacag
    12421 cacggcgaca gtagcggaat gcgtggcgct gcctggcttg ctatgcgcaa cggagaagcc
    12481 aatgaaacgt tcacaaatta attatgttat tgataaagca catgccattg cgcagacctt
    12541 ccgcgtttgt ttgccagagt tcgcctattt tacggtggat gactggctgc agcgggaacg
    12601 ggataactgg caagaagtgg tcgacttaca gctcggttgg gatatcaccg attttggtcg
    12661 tggtgatttc aaccaaacgg gactaacact gctaacgatg cgcaacggcg cgctggggtc
    12721 ggcggcatat ccaaaaccgt atgctgagaa aatgttgcag atccaacaag atcagcagac
    12781 tccctggcat tttcacaccc acaaaatgga agatatcctc aatcggggcg gtggagactt
    12841 atgcatgcag ttggcctggg ccaacgaagc caatctctgt gatgatcaac gagtcattac
    12901 cgtgagtgtt gatggtcagc ggcgtaccat gaaacctggt gaaacgctgg tgctaaagcc
    12961 ggggcagggc atttgcctgc cgccgcgtct gtatcatcgc ttctgggcgg aaaaagcgtt
    13021 tgttctcggc tgggaaatct cgatggtaaa tgatgaccaa cacgataatt attttcttga
    13081 gccgggagga cgttttccgg cgatagaaga agatgaaccg gtgaaatggt tattgtgcgg
    13141 tgagtatgga attttgcgct aataacgggg aacgctagcg ctgatatccg ctagcgtcct
    13201 tatttttact gcaccggctc cgtcgacgta acttcaccac tggaaactac gcccgcattc
    13261 atggtttgag tcacttgctg ctttgcggca ttcacggcat gcaattgccc gctaaccgta
    13321 ggtttcagcg ggacattgac ctgaatatcg ccactggcgg taagctgaat attgccgtcg
    13381 ccagtcaacg gtaattcagg ccatccccaa tgttgcaaaa tattcaccgg cacaccgcgt
    13441 ccattcaggc tgatatgggt ctgacgttgt ggcgtttgtg aaacactggc ggtggcttcc
    13501 agaatgcctt tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct gctgttggca
    13561 gttagcgcca gcgacggacg acggacatca acacgattaa acgttgcggc ggcggcattc
    13621 agattcgccg agccactcca gacgccccat ttatgatcgg taaccagcgt cagattggca
    13681 ccgtaaccat cgagcgcggt gagctgccac gggaagtcag ggtcgatatc aatgatcaga
    13741 ttgcggctgg cgctaaatct cttcagttgc aggctgttta accaaccggg tgtcgtttcc
    13801 atccacaact gttgccagtt tttgggcaag gtatattcca gtccggcaat tgccgcatca
    13861 tcgaggatca gcgttttccc gtcgcgttgc cagtttcctg acgttctgac cataccccct
    13921 tcccagcggc tggtgaactg gcgcagtgct acgccctgcg gggaaaattc cgcgtttata
    13981 atcggatcaa ataaatgcag cgagccataa ataaactcgc tggcgttcat cgacagttta
    14041 ccttcctgtg tctgccagtc atctttactg aaggtcatgt tgcgcaagct gagatcgagg
    14101 tcgaccaccg cccagtccgg accttgcaaa cgagcgtcga tgacttccag gcgaccaatc
    14161 tgcaacgacg ggacagagcg taatggtgca aagaagtcgg ttagcgattt ttcgctttgt
    14221 agacggatat ccgccatgcg caggttttcc acttgccagc tgccgtcagc gttacgctgc
    14281 gcgtttccgg ttaatgtccc gcgggcgatg tcggcaccca ggttagtcag cgtaacgcga
    14341 tcgttatcaa tactgccttc aatcagtaca ttggtggcag gaacatcatt gagcgaaagc
    14401 gatccggcac taaactgaat ctgtgccttc gtacccagca ctttaccggc ttctggtgac
    14461 cacggaacca cgccgccatt tacccgctgc gcgctcagtt tccattcgct attcgggcta
    14521 ttaaacgcca tatcacgcag ttgcagacga tcggctttga acggtagcgg cgcggtctgg
    14581 tcggtgagat tcagcgtccc gttttccagc aggatggtat cgacatggcg tggttcggtc
    14641 agttgccgac tgcttagcgc aatgtcgaca ctttttgcta ccagggtcgc gggctggcca
    14701 tcacggccaa acgtgacgtt ctccagcacg atatgagatg gcgcggaaaa acggtgatcc
    14761 atcgccccga aggccagatg atagtcgcta ttctcggaaa cccatgcgct gatatgttct
    14821 gctccccagc gagtttgcag aagaaaatag aggccagcga tcaccactaa cagagcgatg
    14881 agaatgtaga gaagcagctt cccaataaat ttcatggtct tccatcccgt gaaatgcaca
    14941 taagggagtt atgcacgatt tacgcgcaat cctcaaggcg ggaatggtga aagaggtgag
    15001 actgcggcgg taattatctt accgccgcca gtgaattact gtttttctgg tgggaaaatc
    15061 agattcagca cgatggcagt aataccgccc gcggcaatcc cggaggagag caggtttttc
    15121 agccattcag gagcaaactg caaaatcagc ggctgctgag acacgcccag accgaccgcc
    15181 agcgacagcg cgataatcag aatcgcccgg cggttcagcg gctcacgaga aacgatacgc
    15241 acaccggagg cggcgatggt gccaaacatt acaagcgttg cgccgcccag aaccggttct
    15301 ggaatgtgtt gtacaaaacc gctcactgcc gggaacagac ccagcacgat caacatcagc
    15361 gcgacgacaa aaccgacata gcggctggca acaccggtta actggatcac cccgttgttc
    15421 tgcccgaagc aggagttcgg gaaagtatta aataccgccg agacaaacga gttcaggccg
    15481 tttgccagca cgccgccttt caggcgtttc atgtacagcg gaccggaaac cggctgttcg
    15541 gaaacgtcag aggtcgccgt gatatcgcca atggtttcca gcgaagtgat cataaagacc
    15601 agcatcagcg gcagcagcag actccattca atgccaagac cgtaatagag cggcgttggc
    15661 accataatca gttcttgtgt catcggttcg ttgctttctg gcaacatgcc cataaaccac
    15721 gccagcgcat atccggccgc catcgcaatc accagtgagg ccacgcgtaa gtaagggtta
    15781 cgttgacggt taagcaggat aattaaggct aagaccacgc ctgccagcag cagatttttc
    15841 ggtgcgccga aggtgttatc gctcatggct gcgtaaccgc cgccaatgga ggtcagtcca
    15901 acctgaatta gcgacaggcc gataatcatc accacaacgc cagaaaccag cggcgtaata
    15961 atgcggcgcg ccagatgcag aacgcgggag atcaccatct cggtacaact tgccagcatc
    16021 aacgtgccga acaaagctgc catcatggta ggaacatcag caccaccggt cttcagcgct
    16081 gtgccgccca taatcagcgg agcaacaaag ttgaagctgg tgccctgaat agacaacaac
    16141 ccggagccaa ccggacccca ggccttaatt tgaataatcg atgccacacc ggaggcaaac
    16201 agcgacatac taataatgtg ttgcgtgtct tgtgccggta aacccagcgc ctggcagatt
    16261 aatagcgctg gcgtgatcac cgcaacgaac atcgccagca aatgctgaca ggcggcaaac
    16321 agggtttgag gaagcggcgg acgatcttca agacggtaaa tcagttcgct gttttgagtc
    16381 tgcgcaaccg gttgcgcatt ttctgactcg agggtggaaa cagacatcga aaacaatccc
    16441 gtggtagcaa agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt tgccacatcc
    16501 tcatctaaat atttaaatga gataaatgta cttttttata taacttttag ttgttttctg
    16561 cttaaaatcc atgccatttt tctagtttca gctaagaatt cgaaccagga aagcatgact
    16621 cgatactcgt catatttcgc cttactgcaa atgcgcgatg aagtatgacg agtatgaaag
    16681 agtgatgcgg acacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg
    16741 ccgcaacgct gacgttgctg ctcgggcgca agttggtcca ttccgtctcc tttttgaaga
    16801 aatacaccat accggaacct gttgcgggtg gtttattagt ggcgctggcg ctgctgatac
    16861 tgaaaaaaag catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttgatgc
    16921 tggctttctt cgccaccatt ggcctgaacg ccaaccttgc cagtctgcga gctggtgggc
    16981 gtgtggtggg ggttttcctg attgtggttg ttggcctgtt ggtgatgcaa aatgccattg
    17041 gcattggtat ggctagcctg ttagggcttg atccgctgat ggggctgctg gccggttcta
    17101 ttacgctttc cggcggtcac ggtacgggtg ctgcatggag taaattgttc attgaacgtt
    17161 atggcttcac caatgcgaca gaagtggcga tggcctgtgc aacgttcggt ttggtgctgg
    17221 gcggcttgat tggcggtccg gtggcacgct atctggtgaa acactccacc acaccgaacg
    17281 gtattccgga tgaccaggaa gttccgactg cgttcgaaaa gccggatgtg ggccgcatga
    17341 tcacctcgct ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga
    17401 aaattgttgc gcaacttttg gctggtactg cttttgaact accgaccttc gtctgtgtac
    17461 tatttgttgg cgtgattctg agcaacggtc tgtcaatgat gggcttttac cgcgtctttg
    17521 agcgagcggt atccgtgctg ggtaacgtaa gcctgtcgtt gttcctggcg atggcgctga
    17581 tggggctgaa actgtgggag ctggcttcgc tggcgttgcc gatgctggcg attctggtgg
    17641 tacagaccat cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg atgggcaaaa
    17701 actacgatgc ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa
    17761 cagcaatcgc caacatgcag gcgatcactg aacgctttgg cccgtcgcac atggcgttcc
    17821 tggtcgtgcc gatggtcggt gcgttcttta tcgatatcgt caatgcactg gtgattaagc
    17881 tgtatttgat gttgccgatt tttgccggtt aaccgatgaa gcggcggtct tctcaccgcc
    17941 gctttttacg cattcgagta acgttctgtt tctggcatcc agcgttctat cagggctttt
    18001 gcctgctgtg ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc
    18061 gcctgatcgc gcagtaaatc cgccacttta aattcagcat tccccgtctg acgtgtacct
    18121 aacaattcgc cagggccgcg aatctccaga tctttctgtg caatcacaaa accatcgttg
    18181 ctgtcgcgta gtacctgcag gcggatttgc gcggttttgg agagcggcgt tttgtagagc
    18241 agcacgcagt gagaagccac cgcgccacga cctacacgcc cgcgaagctg gtgtaactgc
    18301 gccagaccca gacgctccgg gttttcgata atcatcagac tggcattggg tacatcaaca
    18361 ccgacttcaa tgacagtagt ggcaaccagc aggtgtagct cgccttgttt aaacgatgtc
    18421 atcacagcct gtttctcggc gggtttcatc cgtccgtgta ccaggccaac attcaactct
    18481 ggtagcgcca gtttcaactc ttcccaggtc gcttccgccg cctgcgcttc cagtaattcc
    18541 gactcttcaa tcaacgtaca aacccagtac gcctgacgcc cttcagtcat acaggcgtgg
    18601 cgcacgcggt caatgatatc ggtacggcgg gtatcaggaa tagcgaccgt agtcactggc
    18661 gtgcggcctg gcggcagctc atctatcacc gaggtatcga gatcggcata cgcagtcatc
    18721 gccagcgtgc gggggatggg cgtggcggtc atgatcaact gatgcgggtg gaagccctgc
    18781 tgctggcctt tctcccataa cgccagacgc tgatgcacgc caaaacgatg ctgttcgtcg
    18841 ataatcacca gcgccaggcc gttaaactgc acctgttcct ggaaaatggc gtgcgtacca
    18901 acaatcatct gcacctgacc gctggcgatg gcctcttgct gagccagtcg tgctttacct
    18961 ttctgttttc cggcgagcca gcctacttcg ataccgagcg gtgcaaacca gttacggaag
    19021 ttattggcgt gctgctcggc gagtaattcg gttggtgcca tcagggccac ctgtttgccg
    19081 tgggcaaccg cacgcaacgc ggcgagggcg gcgaccagcg ttttaccgga acctacatcg
    19141 ccctgcacca ggcgcatcat tggcacatcc agcgccatat cgcgctcgat ctccgccact
    19201 acgcgtgcct gtgcgcccgt tggcttgaac ggtaaggcgg cgaggagttt atttttcagc
    19261 gcgtcattgg tgctcagcgg ctgagcatga aaacgttgtg caccggcacg taacgccaac
    19321 atgctgaggt tgtgcgccag caactcttcc agaatcagac ggcgttgtgc cggatgctgc
    19381 ccggtttcga gatcgctaag ctgcagcgtc ggcggtgggc gatgcaaagt gcgcaacgct
    19441 tccggtagcg tcatcattcc ttgtgacagt tccggcggca ggagttcttc aatggcgcat
    19501 gtgtcgagca gatccagcgc ctggtcggtt aatttacgca gcgtagcctg ctttacgcct
    19561 tccgtagttg gataaaccgg tgtgagcgtt tcctgtaact ctggcgtgct gagatcgccc
    19621 tgcacacggt attccgggtg gatcatctcc gcaccatatt tgccgcgctt tgcttcgcca
    19681 taggccagca cgcggcggcc tgtcgccagg ctatttttca ttgccgcatt gaagttgaaa
    19741 aagcgcatag tgagaatgcc ggaaccgtcg ctgatctggc aggtcatcat ccgccgaccg
    19801 ccgaaggaga tattgcagtt cagcacttcg ccttccaccg tggcataaac acccggcagt
    19861 agttcaccga tggggtagag atgtgtgcga tcttcgtagc gcaggggaag gtgtaagagt
    19921 agatcctgca cggtatgcag gttgattttc gccagcttgt tactaagcgc tgcgccaacg
    19981 cccgttaggg aactgagcgg gacagcatct aacaggcgac ctttcatcgc acttaccctg
    20041 cagcctgcat ggtggaccac cagtcagcat cggcttcaat ctcgccttgc tgattaacgt
    20101 gggggtaggg cagacctttg cgttttgcga ctttcgccag caccggatag ccgccttcaa
    20161 acagcaggcg ttgttgctcg gcttccggca acatgctgtt ttcacgcagg tacatgcccg
    20221 cattttgccg ctggcgctgg gcttcgtaaa gaatgagggc tgaggcaacg gaaacattca
    20281 gcgactgcac catgccgatc atcggaatga tgatatcctg atccgccagg gccaacgctt
    20341 cctgcgtgat gcccgttttc tcctgtccca tcaaaataca ggtcgggcga gtgtaatcaa
    20401 tttcgcggaa atcaacagcg ttatcagaaa gatgggttgc cagaatctgc atgccctggc
    20461 ctttcagatg agcgacggca tcactaatgg tgcggtgcgt tttcacctgt acccagctgt
    20521 tactacccgc agctgccgaa gccatggtgc gcatccggct gccaggccag acagcatgaa
    20581 cttcatgtac gccaacggca tctgcggtac gaataatcgc agaaacgtta tgaggtttgt
    20641 ggacctgctc catgcagacg gtcagatcag gctgccgcct ggcgagcatt tcgcagatgc
    20701 gtgcataacg tgttgggttc ataaaacatt aatttcggtt tcgggtgact ttaatcacgt
    20761 ctggcatcac gcggattttg cgcatgatat tcgccagatg cacacggtca cgggcggtca
    20821 gacgaataaa ggcgctatag acgcgaccat ctttctcttc cgtattcaaa ctttgaatat
    20881 tcgaggtcgt ggtgttaatt gccgccgtca ggtttgccag cgcgccctga tgattgaaca
    20941 tctccacctt gatttcggtg atgaattcct gcgccgtctc tttatcccat tcaacagcca
    21001 taaacttctc tggctctttc tggtagccac ggatgttacg gcaggattca tggtggatca
    21061 ccagaccttt accggggctg acgtgggcga taatcgggtc gccaggaatt gggcggcagc
    21121 atttcgcaaa ggtgatcagc acgccatcgg cacctttaat gggcagatgt ccgtgacttt
    21181 gggttgccgg tggaatggag gcgtccccat gttgcagatt tttcgcgacc accacgctca
    21241 ttgcgttacc aaggccgatt tctgccagca gatcgtcaag cgttgccagc ttcatgcggt
    21301 ccagctcgcg ctgaatattt tcctgcggga tttcattgag cttacggcta ccacccaacg
    21361 catggttgag cagacgacgg cccaggctta cagaatcatc acgcttgagg tttttcagca
    21421 actgacgaat tttggcgcgc gctttcgagc tgacgacaaa gttcagccac gcggcattcg
    21481 ggcgagcacc cggtgcggta atgatttcaa cggtttgacc gctagtaagc ggctgcgaca
    21541 gcgggtaagg ctggcggtcg acgcgtgcgc ccacgcaggc atggccgata tcggtatgca
    21601 ccgcataagc gaagtcgacg ggcgttgcac cggcaggcag ttcgacaatg cgcccttctg
    21661 gtgtgaaaac gtaaatctca tccgggaaga gatcggattt aacgctctcg ataaattcaa
    21721 acgagctacc ggcactctgt tgcagctcca gcaggctttg catccagcgc tgggcgcgta
    21781 tttgcgcggt agtgctggtt tcgccgtgct ctttataagc ccagtgcgcg gcaacaccca
    21841 tctccgccat ctgatccata tcttcggtac ggatctggac ctcaaccggc acgccgtgcg
    21901 ggccaatcat cgaggtgtgc aaagactgat agccgttcgc ttttggaatg gcgatatagt
    21961 ctttcacgcg gcccggacgc ggcttgtaca ggctgtgcat ctggcccagc acgcgataac
    22021 aggtgtcaga atcattgacg atcacgcgga aagcgtagat gtccatgatt gagtgaaaac
    22081 gctgctcttt gagcaccatt ttgcagtaaa tcgaataaag atgcttttcg cgaccactga
    22141 cgcggcacgg tattcccgct tcctgcaaac gcccttcgat ttcagaaaga atcttctgga
    22201 tcatctcttt acggttgccg cgcgcggctt tcaccacttc tttgattacg cgataacggt
    22261 tgggatacag cgcctcaaaa cccagctcct cgagttcggt tttaatgtgg tggataccta
    22321 aacggtgcgc cagcgggctg tatatttcga gagtttcacg ggcgatgcgg cgacgtttgt
    22381 ccgggcgaag tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt ttgatgagaa
    22441 tgacgcggat atcctgcacc atcgccataa tcatcttgcg aaagttttcg gcctgcgcct
    22501 ctttcttatc gcggaacttg agtttatcaa gtttcgacac cccctctacc agctcggcga
    22561 cgcttttacc aaaaagctgt tccatgtcct ggtaggtggc gggtgtatct tcaatcacat
    22621 catgcagcag cgccgccatc agcgtttcat agtcgagttt catctcggcc agaatgcagg
    22681 caaccgccac cgggtgcgtg atatagggtt caccgcttga acgtgtttgc ccctcgtgag
    22741 catcacgtgc aacgagatac gcctgccgca gacgcttgat ttggtcttcc ggcaggtagt
    22801 tttgaatcag ctgattcagg ctttcaaaca gatacaaggg cgacccgctt tgtgattaac
    22861 gacgaccttc agcaatagcg gtaacggctt gtaattcagc ggcttcctgc tcttgctgtt
    22921 cctggcgttc gcgaacgtcg aggatctggt tgttgatcag accttcttcg atttcgcgca
    22981 gcgcgattac agtggtttta tcgttttctt ccggtaccag cggatccttt ccgcctacct
    23041 gcatctgacg agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta ccaattttct
    23101 ctacagcgtc ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg tgaagaaatg
    23161 actgggcatg atactgaaat caggttcagt ctgccaacaa tttgctgatt aaagcgtcat
    23221 gacgctgctt ttggcggctc atgcgcagac gttcggcgcg aataatggtc ttcaaatcgg
    23281 tcaacgcggt atcgaagtca tcattcacaa tcaggtaatc atattcggcg taatggctca
    23341 tttctgcaac agcttgcgcc atacgctttg caatgacctc ttcgctgtcc tgaccgcgac
    23401 cgcgtagacg gcggtccagt tcgattttag acggcggtaa aataaagata ctccgcgcgt
    23461 gcggcatctt ctggcgaatt tgctgcgcac cctgccagtc gatatcgaga aaaacatcga
    23521 caccggtcgc cagtacttgc tcaatggcct cacgcgaagt gccatagtaa ttaccaaaaa
    23581 cttctgcgtg ttcgaggaac gcatctctac taatcatttc tttaaattca tcatgattaa
    23641 caaagaaata atgttcaccg tggacttcac caggacgcgg ttggcgtgtg gtgtgtgaaa
    23701 cagaaacctg ggtgtcatac aacggttggg tttttaataa agcctgaatc aggctggatt
    23761 tacccgcgcc actgggggca gaaacaatat aaagcgtgcc ttgagccatg agtatctttc
    23821 gtatgtgatt agcgaaataa agcctacata cgagcttatt atacacggcg cggccatgtg
    23881 acgtagccct tgctcacact ttttgcgcaa agttgttgca ttttgcgtag ctgcttccgc
    23941 atttgcctgt gattcactgc aataaagacg attctcagga agcgtcctcg cagactccgt
    24001 ttttgccact cggctttgct ttcaccacgt tgtatatctg cctgactatc tacagcggag
    24061 gtgatgatga aagtatggat ggcgatatta ataagtatct tgtgctggca atcatcggcg
    24121 tgggcggtct gtccggcctg gtcgccagcc agagcacagg aagaaatttc ccgcctgcaa
    24181 cagcaaataa aacagtggga cgatgactac tggaaggaag ggaaaagtga ggtggaagac
    24241 ggtgtttacg atcagttaag cgcccgtctc acgcagtggc aacgctgttt tgggaacgag
    24301 actcgcgatg tgatgatgcc gcctttaaac ggtgcggtta tgcatccggt tgcccatacg
    24361 ggtgtacgca aaatggcgga taaaaacgca ttaagtctgt ggatgcgcga acgtagcgat
    24421 ctttgggtgc agccaaaagt tgatggcgtt gcggtaaccc tggtttatcg ggacgggaaa
    24481 ctgaacaaag caatcagtcg cggtaatggc ctgaaaggcg aggactggac gcagaaagtt
    24541 cgcttaattt ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag cacgcttcag
    24601 ggggaaatat ttctcaagcg taaggggcat atccaacaat aaatgggggg aataaatgcc
    24661 cgcgcaaaag ttgctggcct gatgatgcgc caggggaata gcgatacgct aaactcactg
    24721 gctgtttttg tctgggcatg gccggatgga ccgcatttaa tgaccgatcg tttaaaggat
    24781 ctggctaccg caggttttac tttaacgcag acgtataccc gcgcggttaa aaatgctgat
    24841 gaggttgcgc acgtacgcaa tgagtggtgg aaagcaaaat tacccttcgt caccgatggc
    24901 gtggttgtgc gagcggcgaa agaacccgaa tcccgtcatt ggttaccggg ccaggcggag
    24961 tggctggtgg cctggaaata tcaacctgta gctcaggttg ccgaagtgaa ggcgattcag
    25021 tttgcggtgg gtaagagcgg taaaatatcg gtggtggcgt cactcgcacc tgtcatgctg
    25081 gatgataaaa aagtccagcg ggtgaatatt ggttccgtca ggcgctggca ggagtgggat
    25141 attgcgcctg gtgatcagat cctcgtcagc cttgccggtc aggggattcc tcgcattgat
    25201 gatgtggtct ggcgcggagc agaacgtaca aaaccgacac caccagaaaa ccgctttaac
    25261 tcgttgacct gctactttgc ttctgatgtt tgtcaggaac agttcatttc acgcttagtc
    25321 tggctggggt caaaacaggt tctggggctt gatggcattg gtgaggccgg ctggcgcgcc
    25381 ctgcatcaga ctcatcgctt tgagcatatc ttttcctggc ttttattaac gccagagcaa
    25441 ttacagaaca cgccggggat cgcgaaaagt aaaagtgcgc agttatggca tcagtttaat
    25501 ctggctcgtc agcagccttt tactcgctgg gtgatggcaa tgggaatacc gctaacccgg
    25561 gcggcgctta atgccagtga tgaacggtcc tggtcgcaac ttttatttag cacggaacag
    25621 ttctggcagc agcagccggg gactggatcg gggcgcgcca gacaggttat tgaatggaag
    25681 gaaaatgcgc aaatcaagaa gctgggcagt tggctggctg cccagcagat cacaggtttt
    25741 gaaccttagt gaccttcgtg gctgtagtaa aagaccggta atcccagctt aagacgtagc
    25801 gccagtaaac gggcaaaaaa gccgaatacc agggtggaga taatcacaac atcatggtta
    25861 gaaacatagt gttgcagcgc gatgtacagc acggcggagg caaatgagac accggcatac
    25921 aactctttct ggaataccag tggaatgcgt ttacagaaca tatcgcgcaa aacgccgcca
    25981 aacacgccgg tggttaccgc cgcgacaacg gcaataattg ggccgtgccc catatccagt
    26041 gcaacctgtg cgccgatgat agaaaagacc accagtccga gcgcatcgag caccagaaac
    26101 actttgcgca gataaggcat tacaggagca acgatagtgg tgagtactgc ggcggtagcg
    26161 acgataatca catattccgg gtgtttgacc cagccgagcg gatagtggcc cagcagaata
    26221 tcgcgcactg accctccgcc aattgccgtg gcggtcgcaa taataattac gccaaatgta
    26281 tccatgcgcc gtcgtccggc cgccagcgcc cccgtcatgg cttcggcagt aatgccaacc
    26341 aaatacaaaa tgtgtaacag catggttctc tccctaaaaa tctgtctggc agggtagcga
    26401 tttgtgcgta ttgtcacgat tgagattttt taaggtgaga ggaaattgat aacttgtagt
    26461 tatgtgaaat cgctattttc tgtagcgtag atgcattctt ctgacttctt gtctgagaaa
    26521 atgcataaga aaaaataggt taaaattgag gggtggaaaa gaaaggtgca atgtttagcc
    26581 attgtgttca ccgcacctgg actttggctt acgcatcggg tatctcaatc aacaaaaaga
    26641 gtttttattt tctgctattt cttctctgta atttttacac tgttttccaa ctgcttgatg
    26701 ctgacctgcg gggtcggcaa ctcctcaggc atggttccgc caagttcctg aatggtttgc
    26761 ctcaccttgc gacccacgtc aaagtgtgtg gtattagctt gctgttttga attcacgcca
    26821 tcccgcttga gtttttcttc tgtttgggta gctcgaaaga gattagccgc cagttctgtt
    26881 gaacccatat gatccaggat cttctgactc tttttcagcc ccttccgctg atggatggct
    26941 ttctgatcta atccaccata cagtccccgg taaccatgat tctgaaatat ggcgaaatcg
    27001 atagctgtcg ctaccccagc ttgctgcgct gcctcaacta attgtttgtt atgttctttc
    27061 aactcatttc tcagaaaaag acgcttttcg tcttcacgaa gttgcctgaa agcttcatcg
    27121 tcagccagct cctgccgtcg ggtctggata gcaaaataag tttgccctgc cgcaataacc
    27181 ggtttcgcag ggtcgccgtt ttgtactacc aggtaacagg catagcggga gagatgaaca
    27241 tcttcaagtt ctcgttgagc ccctgaacct aatacaacca ttttggtggt ttcaacgaaa
    27301 tggtcagatg cagcttgatt gctggcttcg cacgcttgag tcgcgcgggc cagtaccttt
    27361 tgaaaattgc gccagtcccg ataatccagt aacggtgcca attctctcgc tgaccaaaat
    27421 tcttgccctt cagtgccata atgccgtatt tcttcaaaag gttgatgatg ttcgttcatt
    27481 gcccactcca tatgatatcg attgcaagga aagtaagttt acaactgtgt ttatatacag
    27541 ttgtgagtat ctcacgttct cactgcgctg ggcaaaaaaa catcgcaaaa tctggagcag
    27601 gcacgcaaaa cagaattgaa tgttgcgggg aggaaaaatg ccgctctgtt tgagtgggcg
    27661 gcatttgtga aggctgaaaa ggaagaattg gcgttacgag ttattcaatg ttctgaatct
    27721 gctcgcgcat ctgctcaatc aacactttca gctcgatggc ggagtttgtc acttcggcgt
    27781 tgatagattt cgacgcaaga gtgttcgact cgcggttgaa ctcctgcatc ataaagtcca
    27841 gacgacgacc aacggcttct tttttcttca gaatgttgta ggtctctttg acatgcgctt
    27901 tgaggcggtc cagttcttcg gcaacgtcaa ttcgttgtgc cagcagaacc aactcctgct
    27961 ccagacggtt gttttccagt tgcacctgag catcttccag cttcgctacc agacgctcac
    28021 gctgccattg caggatttcc ggcatatggg cgcggacttt gaccacttcg gcggtgacgc
    28081 cttccagacg ctgctcaatc aacactttca gcgcctgacc ttcggtttcg cgcgcgatga
    28141 taaagtcatc cagggtgcca tccaacgccg cgagaatttc agctgcaatc gcgtcgagat
    28201 cttgctcctg ggctgccatc acgcccggcc agcgtagaat atcaaccggg ttgatttctc
    28261 cttcgtcgct ctgcattttt acccagttcg cggcagttac cagctgttta gccagttttt
    28321 cgttgaggat cagctcacct tgcgcgctaa catctggctc atagcgcaga gtacattcca
    28381 ctttaccgcg cgtcaggcga gaacgaatac gctcgcgaac gacaggttca aggctacgga
    28441 actgctccgg cagacgaaag taagtttcca gataacgctg gtttaccgag cgcatttccc
    28501 aggttgcgct cccccattca cccttgattt cacgccgggc gtaggcggtc atactgcgga
    28561 tcatagacgt tcctgtttat aaaaggagag gtggaaggat tatagccatc gatgccttgt
    28621 caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt ttcaagccgg
    28681 agatttcaat atgcgtccag caggccgtag caataatcag gtgcgtcccg ttaccctgac
    28741 tcgtaactat acaaaacatg cagaaggctc ggtgctggtc gaatttggcg ataccaaagt
    28801 attgtgtacc gcctctattg aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg
    28861 ctggatcacc gcagagtacg gtatgctgcc acgttctacc catacccgta acgcgcgtga
    28921 agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga tcgcccgtgc
    28981 tcttcgcgcg gcagtagatt tgaaagcact gggtgagttc accattacgc tggactgcga
    29041 cgtgcttcag gctgatggtg gcacgcgtac cgcttcgatt acgggtgcct gcgtggcgct
    29101 ggcagatgcg ctacagaagc tggtggaaaa cggcaagctg aaaactaatc caatgaaagg
    29161 gatggtagcc gcggtctctg tcgggattgt gaacggcgaa gcggtttgcg atctggaata
    29221 cgttgaagac tctgccgcag agaccgacat gaacgtagtg atgaccgaag acgggcgtat
    29281 cattgaagtg caggggacgg cagaaggcga accgttcacc cacgaagagt tactcacctt
    29341 gttggcgctg gcccgagggg gaatcgaatc cattgtagcg acgcagaagg cggcgctggc
    29401 aaactgattt ttaaggcgac tgatgagtcg cctttttttt gtctgtagaa aagtaagatg
    29461 aggagcgaag gcatgaaacc atatcagcgc cagtttattg aatttgcgct tagcaagcag
    29521 gtgttaaagt ttggcgagtt cacgctgaaa tccgggcgca aaagccccta tttcttcaac
    29581 gccgggctgt ttaataccgg gcgcgatctg gcactgttag gccgttttta cgctgaagca
    29641 ttggtggatt ccggcattga gttcgatctg ctgtttggcc ctgcttacaa agggatcccg
    29701 attgccacca ctaccgccgt ggcgctggcg gagcatcatg accttgacct gccgtactgc
    29761 tttaaccgca aagaggcaaa agaccacggt gaaggcggta atctggttgg tagcgcgtta
    29821 caaggacgcg taatgctggt agatgatgtg atcaccgcag gaacggcgat tcgcgagtcg
    29881 atggagatta ttcaggctaa tggcgcgacg cttgcaggcg tgttgatttc gcttgatcgt
    29941 caggaacgcg ggcgcggcga aatttcggcg attcaggaag ttgagcgtga ttacaactgc
    30001 aaggtgatct ctatcatcac cctgaaagac ctgattgctt acctggaaga gaagccagaa
    30061 atggcggaac atctggcggc ggttaaggcc tatcgcgaag agtttggcgt ttaacgaaac
    30121 tcgccggatg aaaagtcatc cggcgtcata ttactgcaac tgtgcggcaa ttagcggcca
    30181 gcgggcgtca aaatcatccg tcgggcggta tttaaactcg ctgcggacaa aacgtgacag
    30241 cataccttca cagaaggcca ggatctggct tgccagcagg gtttcatcgg tggtgtaacc
    30301 ttcaccctca cgcattctct tttcacgcaa tacctgacgc aactgcgctt caatacgctc
    30361 gaacagctgg ttgatgcgcc cttgcagacg atcctgttcg aacattagcg catgaccagt
    30421 gaggatgcga gtcaggccag gattacgctc accaaaaccg aggagcaaca acacgatcag
    30481 acgcaggcgc gctgtggtgt ctttctcatc tttcagaatc aggttgatac gagtaatcag
    30541 gctatcttcg ataaactcaa tcaggctatc gaacatgcgg gtcttactgg ggaagtggcg
    30601 atacagtgcc gcttcggaaa cgccgacaga ggcggccagt tttgccgtcg tgatacgttg
    30661 gcttccatcg ctggattcca gcatcagcgc cagagactga agtatttcct cgcgacggtt
    30721 ccttttcgca gtttgttttt ctgccatgtt acaaaatacc cctgaaaata agcacttgcc
    30781 aggcggcacc cacgctatga ccgcaaacga aatgtttgcg gctatgttat gacgttattc
    30841 ggatgcgtat gtgttactga cgaccagagt gaccaaagcc gccttcaccg cggtcggtgg
    30901 cgtcgaaatc ttccaccaga ttaaattcag cctgtactac cggaacaaaa atcatctggg
    30961 cgatgcgttc gccaggttga atggtgaagc tgtcctgacc acggttccac acggaaatca
    31021 tcaactggcc ctgatagtca gaatcgatca atcctaccag gttaccaagc acgataccgt
    31081 gcttatgtcc caatccggag cgcggcagca tcattgccgc cagtgaagga tcggcaatat
    31141 gaatcgccag cccggtcgga accagcgtag tgtcacccgg agccagttct acggcgtcgt
    31201 cgagacaggc acgcaggtca agtccggcag agccagaggt ggcataagtc gggagcggaa
    31261 attccttccc aacgcgcggg tccagaatct taacgtcgat ttttttcatc ataacgggtc
    31321 acgatctcgt cgagtaataa ttggccaagg agcgctttgc gctcaagcgg taagacttta
    31381 tctccgtcct gccagaaaag gtgtaatgcg ttgttgtcgc tgttaaatcc ttgagttggc
    31441 tgggaaacat cgttcgcgca gatcagatca aggtttttac ggatacgttt ttgccgggcg
    31501 tattcttcca cattatttgt ttcggcggca aatccaacga cgtagggtcg atggtctttt
    31561 agtgcggcaa cgcctgcgac gatatcgggg tttttaacca tttttattgt taattcatca
    31621 ccctgcgtgg cctgcttttt gattttttct ggggccacgg tagctgcgcg ataatccgcc
    31681 acggcggcgc aaccgataaa aatattttgc tgctgtacag aagcattcac ggcggcttcc
    31741 atttccagcg cggtcatcac atcaacacgg ttaacaaacg gtggcgtcgg tagtgaaacc
    31801 ggacctgata ccagcgtgac gttcgcgcca cgacgggctg cggcggcggc gatagcaaaa
    31861 cccatcttgc cggagctgtg attagagata taacgcacgg gatcgagcgg ttcacgcgtc
    31921 ggaccggcgg taatcataat gttcagatgt ttcaggtcgt tgacgggcga aaaatgcgct
    31981 accgccatat ccacaatggt taacggatcg agcattcgtc ctgggccgat atcaccacaa
    32041 gcctgactgc cgctgtctgg cccccagatg agcaaaccac gtgaggcaag cacctctaaa
    32101 ttatgctgcg tggcagcggc acggtacatc tgctggttca tggcggggag cacggctaca
    32161 ggcgcaggtg tagccagaca aatcgtcgat accaggtcat tcgccattcc ggcagcaaca
    32221 cgggcaatta aatctgccgt ggcaggggcg agaatcacta aatcagccca tttacccagc
    32281 tcaatatggc ccatagcggc ttcggctgcc gggtccagca gactgtcgga aacgggataa
    32341 ccagaaaccg cctgcaagct aagtggggtg ataaaggctt ttgccgcttc ggtcatggct
    32401 acgcggacat cggccccgcg atcgcgcaaa cgacgcacca gttcaggggt tttataggca
    32461 gcaataccgc cgctaacgcc gagaacgatt tttttaccgg ccaggctcat gatgattttt
    32521 cctgttgggt gacaccagaa gttggcgatt ttatcacaat acttttgttg tcgtgccttc
    32581 acctgagatt cactttgcga ggcgctttcc aggattgaaa actggccgtc gatttaaagg
    32641 aacggctatg acaggatgcg agcaccacaa aggaggtgaa ggtgaaaaac aatgcacagc
    32701 tgttgatgcc gcgcgaaaaa atgctgaagt ttggtattag ctccttaacg gatgtcgagc
    32761 tgctggcgct atttctgcgt accggaacgc gcggtaaaga tgtattaacc ctggcaaaag
    32821 agatgctgga gaatttcggc tctctttatg gcttgttaac ctctgaatat gagcaattta
    32881 gtggcgttca tggaattggc gtggcgaaat tcgcccagtt aaaggggatt gccgaactgg
    32941 cgcggcgtta ctacaacgta cggatgcgtg aagaaagccc tttactcagc ccggagatga
    33001 cgagggaatt tttacaaagc cagctcacgg gcgaggagcg ggagatcttt atggtgatct
    33061 ttctcgactc ccaacacaga gttataacgc atagccgtct tttttccggc acgctaaacc
    33121 atgttgaagt ccatcctcgg gaaattatcc gcgaagcgat aaaaataaac gcctcggcgc
    33181 tgatccttgc gcataatcac ccttcgggtt gtgctgaacc cagtaaagcg gataaactca
    33241 ttactgaacg gataataaag agttgtcagt tcatggattt acgcgtgctc gaccatatcg
    33301 tgattgggcg tggagagtat gtttcttttg ccgaacgcgg ctggatttaa cccgctatgc
    33361 gcgatccttc gggatctttg tctgttcggg acttgagcac atcgctgagt cagcgtatac
    33421 tacgccacct ttgagaatct cgggtttggc atttgggcct ggcaatcgag agttcacata
    33481 gaactgcgat gaccgggctg taaagcctga cgaggcgcca ataccccata cgaagctcga
    33541 gctaatttga tttttggaga atagacatgt cccgagtctg ccaagttact ggcaagcgtc
    33601 cggtgaccgg taacaaccgt tcccacgcac tgaacgcgac taaacgccgt ttcctgccga
    33661 atctgcactc tcaccgtttc tgggttgaga gcgagaagcg ttttgtcacc ctgcgcgtat
    33721 ctgctaaagg tatgcgtgta atcgataaaa aaggcatcga tacagttctg gctgaactgc
    33781 gtgcccgtgg cgaaaagtac taagtactta gaggaaataa atcatggcta aaggtattcg
    33841 tgagaaaatc aagctggttt cttctgctgg tactggtcac ttctatacca ctacgaagaa
    33901 caaacgtact aagccggaaa aactggaact gaaaaaattc gatccagttg ttcgccagca
    33961 cgtgatctac aaagaagcga aaatcaaata attctcgctt tgatgtaaca aaaaacctcg
    34021 ccccggcggg gttttttgtt atctgcttgc ccccatattg actgcatctg ttcattcctg
    34081 gagatgctat gcctgaatta cccgaagttg aaaccagccg tcgcggcata gaaccgcatc
    34141 tcgttggtgc aaccattctt catgcggtgg tgcgcaacgg acgcttgcgc tggccggttt
    34201 cagaagagat ctaccgttta agcgaccaac cagtgcttag cgtgcagcgc cgggctaaat
    34261 atctgctgct ggagctgcct gagggctgga ttatcattca tttggggatg tctggcagcc
    34321 tgcgcatcct tccagaagaa cttccccctg aaaagcatga ccatgtggat ttggtgatga
    34381 gcaacggcaa agtgctgcgc tacaccgatc cgcgccgctt tggtgcctgg ctatggacca
    34441 aagagctgga agggcacaac gtgctggccc atcttggacc ggagccgctt agcgacgatt
    34501 tcaacggtga gtacctgcat cagaagtgcg agaagaaaaa aacggcgatt aaaccatggc
    34561 tgatggataa caagctggtg gtaggcgtag ggaatatcta tgccagcgaa tctctgtttg
    34621 ctgcggggat ccatccggat cggctggcgt catcactgtc gctggcagag tgtgaattgt
    34681 tagctcgggt gattaaagcg gtgttgctgc gttcgattga gcagggtggt acaacgctga
    34741 aagattttct gcaaagtgat ggtaaaccgg gctatttcgc gcaggaattg caggtttacg
    34801 ggcgaaaagg cgagccgtgt cgggtgtgcg gtacgccgat tgtggcgact aaacatgcgc
    34861 agcgggcaac gttttattgt cggcagtgcc agaagtaatt gatgcgagcc ggatggcata
    34921 ccatccggca taaacgctac gctaacttcg ccatcagcgc ctgatggaca ttctccggca
    34981 ggaaatgggt gacatcgccc tgatggcgcg ccacctcttt caccaacgat gaagagataa
    35041 acgaccactc tttcgacggc ataagaaaca cactttccag ttccggcatt aagtggcgat
    35101 tcatatgcgc cagctgcatt tcatattcaa aatccgccac cgcacgcagg ccacgaatca
    35161 gcaccgtagc gtgttgatta cgggcgaagt tcgccatcaa atcactaaac ccgaccactt
    35221 ccacgttccc cagatgcgcg gttgcctgct gtgccagctc cacacgctct tccagggtaa
    35281 acatcggttt tttactgggg ctggcggcaa tcgccagaat aacgtgatcg aacatttgcg
    35341 tggcgcgcgt cacgatatcg atatgaccat tggtaatggg atcgaaagta cccggataaa
    35401 tcgcccgttt ttgcataaca acctcaatgc gttttcggtg gcaggtaagg ttccagcagt
    35461 tgaagcagac gctgtagtgc gccctggttt tgatacagca cttcaacggc atgacggcca
    35521 tagaaactac ggtaatcggc gtcggtgagt aaagaggaaa cctcttttgc aagcgtagtg
    35581 gcatcggtaa cggtaatcag cccgcttgcc tgctccagcc gcgcgcaaat gtctttaaag
    35641 ttaaacgtat gcggccccat caataccgga atagcgtgtg cggccgcttc cagcggatta
    35701 tgcccaccac gttcaaccag tgaaccgcca acaaaggcga gatcggcaat gccgtacagt
    35761 aacatcaact cgcccatcgt atcgccaacc acaacctgcg tgctggtgga ggggacttcc
    35821 cctgaagatc gtgtgatata gcttagtcca gcctggcgga caaggttaat cgcatccggg
    35881 aagcgttctg gatgacgggg taccaggatg agcaataaat tcgggaattg ctgtaacaat
    35941 gcctgatgtg cggcgatcac cacactctct tcgccttcat gagtgctggt ggcaatccat
    36001 accgggcggt gtggtgccca ttggcggcgc agcgtcacgg ctttagcagc caactgcggc
    36061 gttacagaaa tatcgaattt caggctaccg gtaacggtca cctgattatt ttttgcgccc
    36121 aacgccacaa aacgagcacc atcttcttca ttttgggcgg caatcagcgt aatacgacgc
    36181 agcaagcgac ggacgaattt acccagtttg gcataacctg cggccgagcg ggcagagagt
    36241 cgcgcgttag cgatcaccag cggaatttta cgtttatgta gcgccgcaat caggttaggc
    36301 catagttcgg tttccataat caacaccagt ttagggtcga ctttattcag gaaacggttg
    36361 agtgcatcgg gcaggtcata cggcagataa acgtgctgaa catccttccc gaaagccgat
    36421 tgtacgcgct ccgaaccggt tggagtcatg gttgttacgg taatcggtaa atcaggataa
    36481 cgatgacgca gtgcgcgcac caacgggatc gccgccagag tttcaccgac ggagacggag
    36541 tgcagcataa tgccgcctgg ttttagcgga tggcggtaaa aaccgtaacg ttcaccccag
    36601 cgttttcgat aggccggagc cttacgtccg cgcacccaga gccgtatcca gatcagcggc
    36661 tgaataaggt agagaagggc ggtgtaaagc aattcgagca tagtaaatag ctgacttatg
    36721 gatgtgctgg ggattctatg tatttagctg tggctttacc attacttttc ccatttttga
    36781 cttaaatagc aatttacccg ccaattcttg ctgaacatct atactttttt tgtattttta
    36841 tgcgattcat tgatttctgc agtacgtgca ggaaggaatc gcagagcaac agtaaaagaa
    36901 tatggttgct aatgatttta tttgattgat ttctttattg aaaatcaaca atgtgcttaa
    36961 aaatcattcg agtcggattg ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc
    37021 ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc
    37081 ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg aagaattttg
    37141 ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat tagttcgctg
    37201 aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac catcccgatc
    37261 ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa agcaaaagct
    37321 tcagaaaaaa ttaccaactt ttttcatctc atcaaggtat tacgtgccaa taagtatgac
    37381 cttatcgtta atcttaccga tcaatggatg gttgctatac tggttcgctt attaaatgcc
    37441 cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg taatagtttc
    37501 actcatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc cgtgctgacg
    37561 ccattgggac ttgaatcgtt ggtgaagcag acaaccatga gttacccgcc tgcaagctgg
    37621 aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt ggttatccaa
    37681 cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc tgtgattgat
    37741 gccttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa agacgatctg
    37801 gcctgcgtca atgaaattgc gcagggatgc cagacgccac cagtaacggc gctggccgga
    37861 aaggtgacct ttccggaact tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt
    37921 gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc gctgtttggt
    37981 gcgacagacc atattttctg gcgtccctgg tcaaataaca tgattcaatt ctgggcggga
    38041 gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt
    38101 attccggcgg cagatgtcat tgcagctgtc gataaattac ttccctcctc cacgacaggt
    38161 acgtcgttat gatcgttgct ttttgtttat ataaatattt tccctttggc ggtttgcagc
    38221 gcgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt cgggtttata
    38281 cccagtcgtg ggaaggcgaa tgccctgatg tatttgaact gatcaaagtg ccggttaaat
    38341 cgcataccaa tcacgggcgc aatgcggaat attttgcctg ggtgcaaaaa catttacgtg
    38401 aacaccccgt cgatagagtc gttggattca acaaaatgcc ggggctggac gtttattatg
    38461 ccgctgatgt ttgttatgcc gagaaagtag cgcaggagaa aggctttttc tatcgcctga
    38521 cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag ggcaagccga
    38581 cacagctgct gatgctgaca gataagcaaa tcgccgattt ccagaaacat tatcagactg
    38641 aagcggagcg ttttcatatt ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc
    38701 agccagccaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa caacaatatt
    38761 tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac
    38821 ttgcttcgtt accggattcg ctgcgccaca acacattact atatgttgtt gggcaggata
    38881 aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gcgcagtaat gttcacttct
    38941 tctcggggcg caacgatgtc tcggaattaa tggcggcggc ggatttatta cttcatcctg
    39001 cctaccagga agccgcagga attgtactgc tggaagcgat aactgcagga ttaccggtac
    39061 taacaacagc cgtttgtggc tatgcgcatt atattgtcga tgccaattgc ggcgaggcta
    39121 ttgctgagcc attccgccag gaaacattga atgagatttt acgcaaagcg ttaacgcaat
    39181 cttcattgcg ccaggcctgg gcggaaaatg cgcgacatta tgctgataca caagatttat
    39241 acagtctgcc agagaaagcg gcggacatca taacgggtgg tctggatggt tgaacttaaa
    39301 gagccgtttg ccacgttatg gcgcggtaaa gatccttttg aggaagttaa aaccttgcag
    39361 ggtgaggtat ttcgtgaact ggaaactcgc cgcactctgc gctttgaaat ggcgggcaaa
    39421 agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa aaatttactc
    39481 tcattgcgga tgccagtatt aggcgcagac cgcgaatgga atgcgattca tcgactgcgg
    39541 gatgtcggcg ttgatactat gtatggggtg gcattcggcg aaaaaggcat taatccgctg
    39601 accagaacct cgtttattat taccgaagat ctgacaccaa ccatcagtct ggaagattac
    39661 tgtgctgact gggcgaccaa cccaccagat gttcgcgtaa agcgtatgct tattaagcgt
    39721 gtcgcgacga tggtgcgcga tatgcatgct gcgggcatta accaccgtga ctgttatatc
    39781 tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggtgttaaa aatttcagta
    39841 attgacctgc accgggcgca gatacgtgca aaagtaccgc gtcgttggcg ggataaagat
    39901 cttattggac tttatttttc ttcgatgaat attggcctga ctcagcggga tatctggcgg
    39961 ttcatgaagg tgtatttttc tgccccgctt aaagacattc tcaagcagga acaagggctg
    40021 ctgtcgcaag cagaagaaaa agccacaaaa atcagggaaa gaacgattcg aaaatcgttg
    40081 taatttccgg agagaaatat gagtgcccac tattttaatc cacaagagat gatcaataag
    40141 acaatcatct tcgatgaaag gccagcggcg tcagtggcat catcattcca tgttgcttat
    40201 ggcattgatc agaactttct ttttggttgt ggtgtttcaa tcacgtcagt tttgttacat
    40261 aacaacgacg tgagttttgt cttccacgtt tttattgatg atatccctga cgccgatatc
    40321 cagcgtttat cccaattggc gaaaagctat catacctgta tccagatcca tctggtaaat
    40381 tgtgaacggc ttaaggtatt accgacgacc aaaaattggt ctattgccat gtatttccgt
    40441 tttgtaattg cagattactt tattgatcaa caagataaga tcctgtacct ggatgctgat
    40501 atcgcctgtc agggaacctt aaaaccgctg ataacaatgg atcttgccaa taacgttgct
    40561 gctgttgtta ctgaacgcga tgctaactgg tggtcgttac gggctcaaag tctgcagtgt
    40621 aatgaacttg aaaagggtta ctttaattca ggtgtcctgt taattaatac actagcgtgg
    40681 gcgcaggagt ccgtttctgc taaagcgatg tcgatgcttg ctgataaagc cgtcgtttcc
    40741 cgtttaacct atatggatca agatatacta aatcttatcc tgttagggaa agttaaattc
    40801 attgatgcta aatacaatac gcaatttagt ttaaattatg aattaaaaaa atcatttatt
    40861 tgtccaatta atgatgaaac cgtattaatt cattatgtcg gcccgacaaa accctggcat
    40921 tactgggccg gttatccaag cgcgcagcca tttatcaaag ccaaagaagc atcgccctgg
    40981 aaaaatgaac cgttaatgcg gccaatgaac tcaaattatg ctcgttattg c
//
LOCUS       DAZFRE010000031        40729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFRE010000031 DAZFRE010000000
VERSION     DAZFRE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.031"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..98)
                     /locus_tag="ACHKUI_002022"
     CDS             complement(<1..98)
                     /locus_tag="ACHKUI_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFO6598216.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
     gene            341..496
                     /locus_tag="ACHKUI_002023"
                     /pseudo
     CDS             341..496
                     /locus_tag="ACHKUI_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1595..2767
                     /locus_tag="ACHKUI_002024"
     CDS             1595..2767
                     /locus_tag="ACHKUI_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598217.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAIKRHKQQLGAIEEYTQWL
                     KKEPRASYFFLFRLYTRIHNTFFPKKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNSGLTPIPEKRSGM
                     PPEHLRFATGFPPQPKIAGPKGKPMRTVHPDKIYREIIWFCSGYLRKSGPEATRTIIN
                     SIFCEWASIFNDYSSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRR
                     DERADGSVPLKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(3298..3555)
                     /locus_tag="ACHKUI_002025"
     CDS             complement(3298..3555)
                     /locus_tag="ACHKUI_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598218.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(3609..4376)
                     /locus_tag="ACHKUI_002026"
     CDS             complement(3609..4376)
                     /locus_tag="ACHKUI_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6598219.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(4373..5431)
                     /locus_tag="ACHKUI_002027"
     CDS             complement(4373..5431)
                     /locus_tag="ACHKUI_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6598220.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(5450..6439)
                     /locus_tag="ACHKUI_002028"
     CDS             complement(5450..6439)
                     /locus_tag="ACHKUI_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6598221.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGSDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(6450..8615)
                     /locus_tag="ACHKUI_002029"
     CDS             complement(6450..8615)
                     /locus_tag="ACHKUI_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6598222.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            9044..9478
                     /locus_tag="ACHKUI_002030"
     CDS             9044..9478
                     /locus_tag="ACHKUI_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598223.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIHLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            9696..12080
                     /locus_tag="ACHKUI_002031"
                     /pseudo
     CDS             9696..12080
                     /locus_tag="ACHKUI_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            12077..12982
                     /locus_tag="ACHKUI_002032"
     CDS             12077..12982
                     /locus_tag="ACHKUI_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO6598224.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            12979..14049
                     /locus_tag="ACHKUI_002033"
     CDS             12979..14049
                     /locus_tag="ACHKUI_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO6598225.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            14185..14862
                     /locus_tag="ACHKUI_002034"
     CDS             14185..14862
                     /locus_tag="ACHKUI_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598226.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            14878..15288
                     /locus_tag="ACHKUI_002035"
     CDS             14878..15288
                     /locus_tag="ACHKUI_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598227.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKAQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            15509..16330
                     /locus_tag="ACHKUI_002036"
     CDS             15509..16330
                     /locus_tag="ACHKUI_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6598228.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            16593..17066
                     /locus_tag="ACHKUI_002037"
     CDS             16593..17066
                     /locus_tag="ACHKUI_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6598229.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPEYDAIMRIID"
     gene            17082..17558
                     /gene="yeeS"
                     /locus_tag="ACHKUI_002038"
     CDS             17082..17558
                     /gene="yeeS"
                     /locus_tag="ACHKUI_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6598230.1"
                     /translation="MQQISFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKCALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            17627..17848
                     /gene="yeeT"
                     /locus_tag="ACHKUI_002039"
     CDS             17627..17848
                     /gene="yeeT"
                     /locus_tag="ACHKUI_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6598231.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17922..18290
                     /gene="cbeA"
                     /locus_tag="ACHKUI_002040"
     CDS             17922..18290
                     /gene="cbeA"
                     /locus_tag="ACHKUI_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO6598232.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            18379..18753
                     /gene="cbtA"
                     /locus_tag="ACHKUI_002041"
     CDS             18379..18753
                     /gene="cbtA"
                     /locus_tag="ACHKUI_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO6598233.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18750..18944
                     /gene="yeeW"
                     /locus_tag="ACHKUI_002042"
     CDS             18750..18944
                     /gene="yeeW"
                     /locus_tag="ACHKUI_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6598234.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDGRDSTETPATYASPHQLL"
     gene            18990..19070
                     /locus_tag="ACHKUI_002043"
                     /pseudo
     CDS             18990..19070
                     /locus_tag="ACHKUI_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19359..19505)
                     /locus_tag="ACHKUI_002044"
                     /pseudo
     CDS             complement(19359..19505)
                     /locus_tag="ACHKUI_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            19559..19741
                     /locus_tag="ACHKUI_002045"
     CDS             19559..19741
                     /locus_tag="ACHKUI_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO6598235.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(19842..20171)
                     /gene="tmaR"
                     /locus_tag="ACHKUI_002046"
     CDS             complement(19842..20171)
                     /gene="tmaR"
                     /locus_tag="ACHKUI_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6598236.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(20343..21401)
                     /gene="yeeA"
                     /locus_tag="ACHKUI_002047"
     CDS             complement(20343..21401)
                     /gene="yeeA"
                     /locus_tag="ACHKUI_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6598237.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(21599..22072)
                     /gene="sbmC"
                     /locus_tag="ACHKUI_002048"
     CDS             complement(21599..22072)
                     /gene="sbmC"
                     /locus_tag="ACHKUI_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6598238.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDNAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(22191..23357)
                     /gene="dacD"
                     /locus_tag="ACHKUI_002049"
     CDS             complement(22191..23357)
                     /gene="dacD"
                     /locus_tag="ACHKUI_002049"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6598239.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            23566..24993
                     /gene="sbcB"
                     /locus_tag="ACHKUI_002050"
     CDS             23566..24993
                     /gene="sbcB"
                     /locus_tag="ACHKUI_002050"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6598240.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(25067..26425)
                     /gene="plaP"
                     /locus_tag="ACHKUI_002051"
     CDS             complement(25067..26425)
                     /gene="plaP"
                     /locus_tag="ACHKUI_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6598241.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(26415..26477)
                     /gene="yoeI"
                     /locus_tag="ACHKUI_002052"
     CDS             complement(26415..26477)
                     /gene="yoeI"
                     /locus_tag="ACHKUI_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6598242.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(26692..27621)
                     /gene="yeeY"
                     /locus_tag="ACHKUI_002053"
     CDS             complement(26692..27621)
                     /gene="yeeY"
                     /locus_tag="ACHKUI_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6598243.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(27667..28491)
                     /gene="yeeZ"
                     /locus_tag="ACHKUI_002054"
     CDS             complement(27667..28491)
                     /gene="yeeZ"
                     /locus_tag="ACHKUI_002054"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6598244.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(28574..28828)
                     /gene="yoeB"
                     /locus_tag="ACHKUI_002055"
     CDS             complement(28574..28828)
                     /gene="yoeB"
                     /locus_tag="ACHKUI_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO6598245.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(28825..29076)
                     /gene="yefM"
                     /locus_tag="ACHKUI_002056"
     CDS             complement(28825..29076)
                     /gene="yefM"
                     /locus_tag="ACHKUI_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO6598246.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            29359..29409
                     /gene="hisL"
                     /locus_tag="ACHKUI_002057"
     CDS             29359..29409
                     /gene="hisL"
                     /locus_tag="ACHKUI_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6598247.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    29385..29508
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            29555..30454
                     /gene="hisG"
                     /locus_tag="ACHKUI_002058"
     CDS             29555..30454
                     /gene="hisG"
                     /locus_tag="ACHKUI_002058"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6598248.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTDRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            30460..31764
                     /gene="hisD"
                     /locus_tag="ACHKUI_002059"
     CDS             30460..31764
                     /gene="hisD"
                     /locus_tag="ACHKUI_002059"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6598249.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALCEYSAKFDKTTVTALKVSADEITAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVRDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSVLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            31761..32831
                     /gene="hisC"
                     /locus_tag="ACHKUI_002060"
     CDS             31761..32831
                     /gene="hisC"
                     /locus_tag="ACHKUI_002060"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6598250.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECHTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLITTLKEIPCVEQFFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            32831..33898
                     /gene="hisB"
                     /locus_tag="ACHKUI_002061"
     CDS             32831..33898
                     /gene="hisB"
                     /locus_tag="ACHKUI_002061"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6598251.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLRLQ
                     KAGYKLVMITNQDGLGTQSFPQVDFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALCDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            33898..34488
                     /gene="hisH"
                     /locus_tag="ACHKUI_002062"
     CDS             33898..34488
                     /gene="hisH"
                     /locus_tag="ACHKUI_002062"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6598252.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            34488..35225
                     /gene="hisA"
                     /locus_tag="ACHKUI_002063"
     CDS             34488..35225
                     /gene="hisA"
                     /locus_tag="ACHKUI_002063"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6598253.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            35207..35983
                     /gene="hisF"
                     /locus_tag="ACHKUI_002064"
     CDS             35207..35983
                     /gene="hisF"
                     /locus_tag="ACHKUI_002064"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6598254.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            35977..36588
                     /gene="hisIE"
                     /locus_tag="ACHKUI_002065"
     CDS             35977..36588
                     /gene="hisIE"
                     /locus_tag="ACHKUI_002065"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6598255.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMTPEALDR
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(36774..37751)
                     /gene="wzzB"
                     /locus_tag="ACHKUI_002066"
     CDS             complement(36774..37751)
                     /gene="wzzB"
                     /locus_tag="ACHKUI_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6598256.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(37897..39063)
                     /gene="ugd"
                     /locus_tag="ACHKUI_002067"
     CDS             complement(37897..39063)
                     /gene="ugd"
                     /locus_tag="ACHKUI_002067"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6598257.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(39312..40718)
                     /gene="gndA"
                     /locus_tag="ACHKUI_002068"
     CDS             complement(39312..40718)
                     /gene="gndA"
                     /locus_tag="ACHKUI_002068"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6598258.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILSKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT        10348 a        10521 c        10309 g         9551 t
ORIGIN      
        1 gtattgcggg agatccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc
       61 aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag
      121 cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt
      181 gacacaatgg gccatacggg ccgacgttcc atgccgcgtt actacattca gcccggacgt
      241 aaaatgcgcc cgtcaaaaag aacggttcgc ttcgaaactc agccaggata ccacctctcc
      301 gccacacccg ggaagaagtt gatatggtgg ttgccggaca acagagctcc tggattgact
      361 gctgcatcac tcaaccacgc tgaatatcaa aggagagcgt taccggttaa aagagaaacg
      421 taaagctgtg gtgatgaccc aaaacgcaac gcctgtcagt gaggatgaaa tggtgaaaag
      481 cggacagcac cagtaacgga agtatcttag cgggtacgaa aatggcagga tcgtgacatt
      541 gacagaatat ctcctgatca tcctgaatga ctgcagttgc tgtggaaaag taagctgtct
      601 ccacccacgg cacgacttaa caggcattcc gtaactcccc cagactgact atgacgcatt
      661 atcggtgcac ttaaaaccgc agaccgcatt gcgggtgtta ctgcatgaca gtctgaccgt
      721 attgtgagat gtcggcagga accgatgtcc gcgcaggggg gaacttcctg cagtacctga
      781 cgcttcaaat agtattaacc gtagggcgtt gttggttccg gatcgcggaa atgggagcac
      841 aggtctgcta cggggtgtac ctctgacagt aaatccggct cgctcatctg acagcctctc
      901 tcccggaaaa gaccaaaaat gagatcgtct cctcaacagc agagaagagg tcttgacaga
      961 caggctacat tgcatacgat ttatgaagaa aaagacagca aatatggagg ttatagcgga
     1021 gaacacccta cgaaattcct atgaaaaacg attgaaaaaa atatcaaatt cgattcgttt
     1081 ttatatgctt tttgggaacg tggcgatgac gaactgttgc atgtgcagag gagaatgcac
     1141 ccggaagcac cagaatcatc cagaaaggca gaacgattac gcatcaaacc aggagacagc
     1201 aatatcaagg taatacactg atttaaaatg aatttacagc aatacgtgaa aacatttttt
     1261 acttaaagga gacagatgcg ctgaagagag tgcaggtatt tttatataaa aatgagaaga
     1321 ttacgggata tatatccggc acactatgac cggagaatac cagtcaggct taaaaagaca
     1381 ataaatacag tgagttaaat acacagagaa aacgaaaacg tagtatcaga gggtacatct
     1441 tacaacagag actgaatttc cggtttttca taaccggcca ttccggaaag tgcaggcggc
     1501 ccggagctct tctgacgcca gtacctgcca gattgttctg tacattctgt acatcatgta
     1561 cggaggtttt tgtttgcttt cttaaaatct gcaaatgtgg gaagagcagg tgaggtgtat
     1621 tgtgggaaag gaactggatg atatctgcac cagctgtccg tatatcgatg ctataaaacg
     1681 gcacaaacaa cagctcggtg ccatagagga atatacacag tggttaaaaa aagaaccacg
     1741 ggcatcttat ttttttctgt tccggctgta tacccgcatt cacaatacat ttttccccaa
     1801 aaagcaacaa ctcccgttca ctcccggtgg cactcactgc ccggaacctg atgtcaccct
     1861 tcgggactta acgctttccc cgggctacca cagtgattat gcccctcaac ccattccgga
     1921 gatggacagt tctgctgttg ttcccccaac taatgaaaac acctcgcctc cggaagatac
     1981 tcccgacaac actccggccg gaggcaatac cggacaagcg gaaaagacca gaaactcagg
     2041 actcacaccc attcctgaaa aacgttcagg aatgcccccg gaacatctgc ggtttgccac
     2101 agggtttcca ccgcaaccga agattgccgg tcccaaagga aagccgatgc gcaccgtgca
     2161 tccggataaa atctaccgtg aaattatatg gttctgctcc ggttacctgc ggaaatccgg
     2221 accagaggct acgaggacga tcatcaactc aatattctgt gaatgggcca gcatcttcaa
     2281 tgattactcc tccccctttt cgtgggtgga cagcagggac agtgaacagt gtgactggtt
     2341 atggaacgcc atgcaggtcc ggtgtgtggg aaccccgctg aatcccctta ccccggagca
     2401 gaaatactgg ttcgcctgcg ccacgtttga taactgggaa ggctggaatg agcaacaggt
     2461 acagttttta ctggaaagta atcccagacg gaacagagcg aagtttacgc aggcctcctt
     2521 ccaggccccc aggattcagc ataaagcgat tcttcttgat gagctgaaaa gtgcccggga
     2581 gcaacaaaaa aggcgtgatg aacgtgctga tggttccgtc ccgctgaaac tgtccggaaa
     2641 aatccacaaa cagcttgaaa gtattgcccg aagtcgtggt gtcctcccaa aaaaactgct
     2701 gaatgaaatg attgagcagg cgtaccagga ctttgtggca aatgagcaac acaagacact
     2761 gagttaagga ccgcagggcc tccggttacc gattctgcag catacacaac gaatgtgtcc
     2821 ggctcttacc tgtgggggcc gcactaaagt tacgcattga gtatgagtgg gtaatcccgg
     2881 atgagaatat cccggttttt acactattca ctgaaggtgc gacatatcat tcagtctctc
     2941 tgaattcttc atttcagtgg ccccggctac agtcgtttaa tctcctccac gaaccggggc
     3001 cgcaacttgt tgtgtcaata ccaaattgtt ggtcattact gtcttgtgtc actggcgctg
     3061 acaagtgaac tgaaatccgc accttaatat tctgactggc tgcgtacatt catggcatga
     3121 agcggccgga gcggctgaac tgaaggtccg ttgtgagcga acagcggaca ttcactacac
     3181 atggttcaaa catgaaccgg agctttattg tctagctgtc agtgtactgc gatttacccg
     3241 ctcggctgtc cagaaaatcg gtctgaaatc cagtcattaa agggccttag cctgacatta
     3301 agcaacttgg taggcggata gataatagaa ataggaatgc gcaggtctgt catgttgcct
     3361 agaacggtga caagctgtcc ggtgcgaaat cattccggca gcgacaggct catgtgtcag
     3421 cccgacgccc agaccgtcaa cgagcatgtg gatctgcgag gcgttatcgt tcgcagcgat
     3481 gttaaaacga acaatctgct catcttctag aagcacaagt gctttaatga cattaaggcg
     3541 gtctatatgt tgcattacag acttcctatt gatctttacc tgaggctgaa gcaaccacgt
     3601 taatccgact atttttcgat tgcaccatcc actatcacca tcgatcttcc ctgagcacaa
     3661 cgttcaactc tcccgcgtac gccgtatacc tgcgcaaggc tctctgcatg gatcaccgtt
     3721 tgcgggtcgc cgctgtcgat aagtttcccc tctttcagca tgatgacctg ggcggcatgc
     3781 cgcagtgcga tattgatatc gtgtaagacg accagcgtaa ccatattgcg tcttcgcgtt
     3841 tcgcgcgata caacatccat cacatggaac tgataattga gatccagcgc gctcagtggt
     3901 tcatccagca ataataatgc ggggcggcga ataagcgact gtgcaagccc caccagctgc
     3961 ttctgaccgc cggacaggct atcgaggtag ttcattgcca gatgtgcgat gcctagctct
     4021 tcgagcagcg ctatagcctg cgcctgattt tgcccggcgc cggaggcgcg ctgggcgaca
     4081 accaccgact ccagcacctg taaatgcacg ccctgcggca gggactgcgg caggaacaca
     4141 actttttcag cccgacgagc aaacggcagc gataagagat tctcttcatt cagccaggct
     4201 tcgccactgg cgcgatttag cccggccagg gcgcgcagta gcgttgattt accgcagcca
     4261 ttaggcccca gcagtacagt gacttcgccg cgcggcagcg tagaaatcga cagatgctca
     4321 ataatccgcc gtttgccgta accggcgcac agcgcattaa tcgtaagccc gctcatgaca
     4381 tactccctcg atgacgcatt acaatgctca gaaagaacgg aacgcctacc agcgaggtaa
     4441 caattcctac cggtaaaatg acaccgggaa tgatgttctt cgacgctacc gaggccagcg
     4501 atagcaccag cccaccgata aggatgcttc cgggcaaata aaagcgatga tcttctccca
     4561 gcaacaggcg agaaatatgc ggtgcaacca ggccaataaa gccaattggc cccacaaacg
     4621 caaccgaaag ggccgccagc aggctgatgc gcagcaggga acccaggcgc aaacggcgaa
     4681 catcaatgcc aaaactcatc gcccgctctt cgccgaggcg tagcgcggta agctgccagg
     4741 cacgacgcag cgaccagggt aaaacaatcg ccatcgccgc cgccagcacc gccagtttct
     4801 cccatgatgc gcgagccagg ctgcccatcg tccagaacac caggccctgc aaagtatctt
     4861 catcggcgac aaactgcata atggacacca gcgcattgaa ggtaaacacc agcgcaatcc
     4921 caaacagcac aacgcccgac gtcggcacgc gcgtccagcg ggtcagacta tcgagcagca
     4981 gggcggacag gagcgcaaaa agaaaagcgt ttgccggtat aaacccgctt tcgggaacgc
     5041 cgggaatacc gatgcccaga acaatcgcca gcgctgcgcc aaaggccgcc gccgacgaaa
     5101 cgccaagcgt aaacgggctg gcgagggagt tattcagaat agtttgcatc tctgccccgg
     5161 ccaaacccag cgccattcct accaggagcg ccatcagcgc atagggcaaa cggatgtccc
     5221 aaacgataac ccgcatgccg ttagtcgccc cggacggatg catcaaagtc tttatcagcg
     5281 aatgtaaagg gagccctgag gggccgaggg tgaaatcgag cagcagcgaa gcaatgatag
     5341 cgataaaaat aaccgccagt attaccagac gctggcgaaa aagctggcga tagcggttct
     5401 cgaccgacag cgtactttcc aatacagtca tggtttttca cagcctccgt tagcgcggcg
     5461 gattgagcac atcgctcatc tgcgcatcgc ttaacgtcag gccaaagaac tgccgataaa
     5521 actcacgcgt ctctttgcgt atatccacat cagcaaatcg cgcaggataa agcgttttcg
     5581 ccagccacaa aaattgcagc gcctcttcgc tggtttcacg gcaccaccag aacattcctt
     5641 tcggattaac gtaaacgcga tgatgaatga ccgcgtccac gcttgcccac tgcggagaac
     5701 tcagaatggc atccgcgtcg cgcttgttca tcgcgatgat aaccgccgga ttggcagtga
     5761 caattttctc cagcgcgact tcacccgaac ggttttgttg atgctcgcca aaccaatttt
     5821 cagcgatatt tttgccgccg gccaggtcaa tccagtcctg gtttagcgag ggtctaccgg
     5881 tggtggtcag cggattgccc atactgtgat agagcgacac gcgctgtgac gcaggaagat
     5941 ctttcaggcg cccggtgacc agggccacgt tgcgatcgaa gtagcgttga taggcgagcg
     6001 ctttttgctg cgcatcggac cccagcaccg ctgccgtttg ctgtacgcgc tgcgttaacg
     6061 cccgcagtga attcgcctca aacgccagca cctgcacccc agcctgtttc agcaacgcct
     6121 gttgaggcac taccatattc tgcggtacaa aaagcatatc gacccccagc gcgataatct
     6181 gttcgggatt gatttcatgc ccgctgttta cgctaactgc tgcgactttc tctatgcgcg
     6241 gcaggctctg gcgaaacagg gggatccttt ttgcaatcat cgaggttccg acaatgttat
     6301 cgccgtaccc cagcatggca ataatggtat tttgcgccgg ccacggtgac gcgatacgca
     6361 cctccttgtg cgtattttct gcgtgcaggc tggccgccag cagcagcgcc agcagcggaa
     6421 ataaacggga gagtttcatc atttatccat cagaaatcga aggtgacgga tgcacgcact
     6481 tcgcggccgc cgccgataaa cgcacttggg gaaccgttat cgccatcggt acccgatggg
     6541 aagatagaag cccagtaatg tttatcaaac acgttgttta ccaccacgcg gaaagtggtt
     6601 gggacgttgc tcactttcgt ggtgtaacgc gttcccaaat cccatgtggt atagctgctg
     6661 gcgtaagagg tgttggtatc gttcgccgcg cgtttgcccg tataatggac gttagcgctg
     6721 taaacccatt ccggtatgga cggcaaactg tattccgcca acagattggc ctgcactttc
     6781 ggcacgccga caacctgttt attgctggtt gaggcattcg ccgtatcttt cagtttcggg
     6841 tcgaggaagg tcacgccgct gtaaatattc agcccctgcc acacattccc ggcagcggtt
     6901 aactcaaggc cgttgttaac ctggttaccc tgctctttat acacgttatc cgtatcaaga
     6961 taggcaaacg gacgttccag tcggaacagc gcggcgccta gattcatctc gccgatgtcc
     7021 gattttagcc ccacttcata ctgcttgctg cgatacgggt ttagcgtttg accggcattt
     7081 tttacgctct catccgtcgg tgcggtaccg ccctgctcca gcgaatcggc atagctaacg
     7141 taggccattg tgttaggggt aattttatac atcagcgcgg cgttcggact taaaccattt
     7201 tcatcaacct ggttcgtttg attaccgtgc ttatcgtagt ttttagtctg cagccagctc
     7261 tggctgagat agaacattgc cgaccactgc ggcgtaaatg tcaccgtatc gccgagcgta
     7321 acgctctgct gagtatttac gctggagcgg tatcgcggcc cgccggtgcg gattttgcca
     7381 tcgccctgct catctatcgc atccgggtga tacatatttg ttgtacccca gctatagctg
     7441 gaacctgttc ctttggcact ataaagcgac cagacatatc ccgtggtaga aagtgacaga
     7501 tcgtggccga tagagccggt atcaatatga ccattcagcc cggcggtgtt gctcaggacg
     7561 cgaaaacgtc cggcagcggt ggaatccttc atcgaacgag agatatcgcc ctgattgttg
     7621 agtattttac tggaaacact acgcatcgcg cggtcagcct gctgccagcc cacgccagcg
     7681 ttcatggacc agtcgtcgtt aaagtagtgg atcaggcgag tgctgatggt atcggtagtg
     7741 aggtcgttgc ccgcagtgct gagcgccaga tttttgtcct tcggattcgg cgcagacggc
     7801 agtttgacgt tcggcccata gttaaagcta ccgacatagc ctttctggat aaattcgtaa
     7861 tggctggcgt cgagctgtag ctgagtgccc ggctgaatat tccagtcgag cgcaacggaa
     7921 acgagtttgc gacgcagcgt gctgtcatcc acattgcctt ccccttcctg atcaagcagg
     7981 ttcacgcgat agccaaaccg tttgttttca tcaaaatggc cacccagatc ggcatggccg
     8041 gtaaacgcac tgcggctttg atagcccagc gtcactttac gcagcgtctc ttcggttggg
     8101 cgcttcgcca cgaaattaaa ctgccctgct gggctcgccg ggccgtacag cgcgccggtc
     8161 aaactgttaa gcacatccat gcgctcaagc atttccaccg gaaacgcggt tgttgaaacg
     8221 atattcagcc cgtccagacg gctgttggcc accacgctgc cctgcatccc ccgactttgc
     8281 ggacgcccga catccattcc accgcgcgcc tgcatttgcg tactgggaga gtatttcagc
     8341 atctcgctga cgctttgcgc ctgctgattt tcaatcatct ctttagttac agtggtgctg
     8401 gaatatggtg tttcgatcca ggatttcgtt cccagcgggc cgacatctat atcgttcgtt
     8461 ttgaaaccgg caccggcggc agaatcggtg gtgtcggcgg cggtgttgcc cgtgacaatc
     8521 atttcatctt cagaagtaga cgaggcatga gcatgaagcg gtatgaaaga cgctaatgct
     8581 ggcaaaagcg cagcgaaagt tatttgagtt ttcatgaatc actgatcgtt atgtaaatat
     8641 ttatattacg atcggtgcat aggcaaaatt atgatccaga tcacacgtga atgtaagtta
     8701 tttacaaatt acgctaacct tctgtctccg ctctttgcag cataaaagta tggcggcgcg
     8761 ttgttgaaag caggagcggc cgggttgtca gaaagctggt ttggacgcaa gtaatatata
     8821 acaactctga ttaccgcata ctgtcaggac agtatctact tgagcgtccg cctttggcac
     8881 aaagcggact gtcagattag attcagacct gtactcatta gctgatacaa agcagccaca
     8941 tatacctgca ttcaaaataa tttcagaaag atattatctc cgtgaatccc ccgccacctt
     9001 tccggtgcac ggggttttgt cttttttcac tgggaataca tgtatgaatc cgtctgatgc
     9061 cattgaggca attgaaaaac cgctctcctc cctgccttac ccgatttccc gtcacatcct
     9121 ggaacatctg cgcaaactca cccgtcacga acccgtgcct ggcattatgg gtaaaagcgg
     9181 ggccggtaaa tcctcactct gtaatgcact gtttcagggg gaggtcaccc cggtcagtgc
     9241 actcatgaca gtgcttcccg gacatgccgc catccatctg atgacacgcc tgcaggacga
     9301 actgcgcacg gagtctgtcc gcactcagac ccgtgaacag tttaccggtg cggtggaccg
     9361 gatatttgac acggcggaga gtgtctgcat tgcctctgtt gcacgcacgg ttctgcgcgc
     9421 cgtccgtgac tcggtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac
     9481 ccggtggtga tgtaccaggc tgttgttaca gatttttaac tccatgaaaa cactatgcaa
     9541 acgcagccat acccggggtg tccccttgcg ttgtgttcct caagttaata gaatacatcg
     9601 atcgttacta ttatattgat cgttataaac gatcgtttgt agtaaatgac ctcctccgtg
     9661 gttgtcatga aaatgcgtct caggaaggtt aaataatgca cgaaaagaac atcgccctgc
     9721 tttgtgatga agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg
     9781 aggagccaga tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga
     9841 aagcactgaa aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga
     9901 tggtgctggc tgttgtcggc acgatgaaag caggcaaatc aaccaccatc aacgccattg
     9961 tggggcagga aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc
    10021 gccacgttcc cggaaaaact gagccggttc tccatctgga acatattcag cctgtccgca
    10081 atttattaat cacactgcag gaaaaactcg ccaccccggc aggacagcag gtcgcacaga
    10141 ccctgcagca aaccggggat acccgcgaac tgctggatat tctgacggat gatggctggc
    10201 tcaaaaatga ataccacggg gaggaggaaa tctttaccgg actggcatcg ttaaacgatc
    10261 tggttcgtct tgctgcggca atggagactg aatttccttt tgatgaatac gcagaagtgc
    10321 agaaactgcc ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg
    10381 gaacactcac actgctggat acccccggcc ctaatgaggc cggacaaccg cagatggaag
    10441 tgatgatgcg ggatcaactg cagaaagcct ctgcggttct ggctgtgatg gattacaccc
    10501 agatgaactc aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat
    10561 cagccggccg cctgtttgta ctggtcaata aatttgatga gaaagaccgc aatggcgatg
    10621 gggcagatgc cgtacgccag aaagttccgg caatgctgaa cagcgatgtg ctgcccgcct
    10681 cccgcgttta tcccggatcc tcacgccagg catacctggc taaccgtgcg cttcatgagt
    10741 tacggaaaaa cggaaccctt cctgttgatg aagcctaggt cgatgatttt gtcagggaag
    10801 ccttcggtcg catgaaaaaa gattacgtct gtaaagacag cgaactggca actgaagggg
    10861 cgacagacct gtgggaatgc tcacttatcg atcaactgat aacggaagtc atactgagtt
    10921 cacactccag agctgcggca ctggcggttg attctgccgc cgcaaaactg atgcagaatg
    10981 cagaaaatat cagtgaatac ctgtcgttac gccatcaggg gctgatgcaa agtattcagt
    11041 cactgcaggc gcatatcacc agcctgcttg aggatatccg ggaaatagca gactgtcagg
    11101 agcaggtgac cgctgatgtc agaatggcca tggaggagat cgatgccaga acccgggaat
    11161 tactgacggg ggtctgcacc tcactggaag aggagctgaa tgactatttc agaagcggta
    11221 aacgcaaaga acagcaaatg ctggaggaag aaaattcagc gcagccccgg gagagaaacg
    11281 cattcgcttt ctttcacgat attttcggaa ccggaaatca gcatgacagg atgcgggact
    11341 ttgatcccga cagccccgaa ataaaattca gtgatcgcag ggaagccctt gaactgatga
    11401 cgcaaatcga atcgaccgtg accagcctgc accgtgaggc tgaagcacag ttccggcctg
    11461 agctggagaa aatcgtcagc gggattgaaa caggttttcg tggcacggcc ctgtacgcca
    11521 cagaaaacat tgccggtcgc atcaataccc gcctggagga tgagggcttt accgtaaaaa
    11581 tcagttttcc ggcagtcagt cagttacaaa cccggctcgc ggtaaaaaca aatctgagtg
    11641 cgcttatgga ggaaagaacg gagaccgtca cccgtcgccg tcggcagagt ggtttatggg
    11701 gaaagatttg tggagcgttt ggcaccagtg actggggctg ggaaacctat aaagaggatg
    11761 tgagccgcag tgttatcaat atcaacaccg tcaggaagga agttatgtca ctgacccggg
    11821 catatttcgg ggagctgcag gcatccattg agcaggatat taaccagccc gtccgccagg
    11881 aaatcgatgc ctttttctgc gcattcaggg agaaagttga acaactgcgt aacacgctga
    11941 ttcagagctc tgaagatcat aaacgcgatc agcaggcaca ggaacggctt accaggcgac
    12001 ttcaggcatt aaatgaaagg gttcctgagc tcattactga cagtaaggcg ctgagggaag
    12061 agctggagac aatgctgtga cttcaccatt tattcaacaa attgctgata accgggtatg
    12121 tcaggtactc acctgtcttc ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt
    12181 tgcacaggag catatccgca cggccggaga gcgcacgttc ttccggcgtt taaaagaggg
    12241 actgaccggc gaaggcgctg cacggcagaa tgccatcaat gcttcggttg cacagggcct
    12301 tgaggcgtcc ctgcgctggc tgacggagct gacaacgtca cttgccacca caaactacgc
    12361 gattacccgg gtaaacgaca gggtcagttc actggtcagt gatactgcca ggctggcgca
    12421 ttattccgca gatacacggg agcagttact catcctggcc gatcaggttc accataaact
    12481 gaatcatctt gaagaaaaac tccaccgtgt tgaccaggtt cagcgggcgc agttacatct
    12541 tgagcagata ttctcatggt ggagcgccgg gcgatatgcg tcattttccc ctgccggacg
    12601 ttgttatgtg gcgcttgaag agcttcgctg gggcgcgttt ggtgatgtga tacgtcaggg
    12661 cgaaacaggc caggttaacc agctactgga tattctcaga cataaagcat taacgcagat
    12721 ggcacaggag agtggcggta gtgcaaccgt gcgtctgaac actctggact ggcttggtgg
    12781 tcagggccgg gagcaggcag ataacgagtg gcatgacgcg attaactggc tgggagactg
    12841 gtgcagtgaa gagcagcatc cggtgatctg gtcaaccacg caggctgcag aacacttacc
    12901 ggttcgcatg cctcgcctct gctctgcaga acgcctctct gaaagtatgg tcgatgaaat
    12961 atttcagaag ggggctgcat gagtacagaa atgaaaacgg ggctggtgct gtccggtggc
    13021 ggggcggtgg gcgcttatca ggcgggagtg gttaaggcac tggcagagtg tggtacacag
    13081 atcagcatgg tttcaggggc cagcattggc gcattcaatg gtgccattat cgcggcctct
    13141 cccgatctgt cagaagctgc cgtacgcctg gaggcgctct gggatcatct ggggaataat
    13201 caggtgctgt cggtaaaccg attggtttac ttttcattgc tgaaaaaatt gttccaggca
    13261 atgaacctct gccagatccc cggacgtgca ggagcactgc ttacgacgct tcttcgccat
    13321 atatcgatac tcaacgggtt tgacaatctg atggctcagc cgttgttgtc agatgagccc
    13381 ctgacagcgc tgttggatca ttatcttgat actgatgctc tggcagacgg gctaccgctg
    13441 tatgtgtcgc tgtaccccac agaagggggc atgcaggata ttattgactg cattcgtgct
    13501 gaactgggtg ccggaaccac gaaaaacgcc gtttttcagc atatccagag cctgccccgc
    13561 ggacagcaga aagaggctct gcttgcgtca gccgcgctgc ccctgctgtt ccgtccccgt
    13621 gaggttcagg ggacaatgtt cggtgatggt ggtatgggag gatggcgaaa tatgcaggga
    13681 aatacccctg tgacgcctct ggtcgatgcc ggatgcaata tggtgattgt gacgcatctg
    13741 agtgacggtt ctttatggga tcgccaggct tttccggaca ccacaatcct tgagatccgt
    13801 ccccggaaaa ggctgaaata tgcaggtgat ggtgacaaca gcggcggtct gctcagtttc
    13861 acgttggcac ataccgacgc ctggcgtcag cagggctatg aagacacgat gctgacgatg
    13921 gagcatatcc ggaaaccgct ggcagcacgt caggtactgt cccggtcaga aacggtgttg
    13981 cagaaaagtc tggagataac ggaagaggca gatttggcac tgagaaacgc gatggcccgg
    14041 attaaataaa gacgccccaa agaaacgcca ccgcctgtgg tggtgttttt cctggctggt
    14101 gttattgaaa atattttcag atatatatta cctgagagaa agttttaccc ctcacctgtc
    14161 aattcactca ctggaaattt aattatgcaa ttacctgtaa agttattaat gagccttata
    14221 tctctggtca gcgttattgc acgtgccggg aaatataaaa attacatccg ggatgaaata
    14281 aaatactggc gatatacatc atacaagggg ggggaatttc cggaaggttt cactgatgag
    14341 aaattttcca gcgccattta caacggaaga atatttacaa tgaaacgttt acataccctg
    14401 atgttatttc tggcggttct gtttactggc tttaacgtgg aagcagcgag cgtgaaacaa
    14461 gcgctcagct gcgacccaaa cgcccgggct gaacaacctg gagcgtgtcc aacaacgtac
    14521 gagttgtacg aaggtgacgc tgcctacaaa gctgcgcttg acaaagcatt aaaaccggtc
    14581 ggactgagcg gcatgttcgg taaaggcggg tatatggatg gccctggcgg aaacgtaacg
    14641 ccagtaacca ttaacggtac agtctggctc cagggcgacg gttgcaaagc caatacctgc
    14701 ggctgggact ttatcgtaac actctataac ccaaaaaccc atgaagtcgt tggctaccgc
    14761 tactttggtt tagatgaccc ggcctacctg gtttggttcg gcgaaattgg cgtgcatgaa
    14821 ttcgcgtatc tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctgtcatg
    14881 aaaactttta tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctacg
    14941 gcgaaacaag taaaactgcc aaacaacatc aaatacgtta atactacaga ggcgttttcc
    15001 tgtactgaga ttgacggtat gaattgccag acgaagaatc cgtttaacta taaagataac
    15061 agctatgttt tcgtgcttga acgtggtggt gcctggtgtt acgactacac tgtctcggta
    15121 cttaacctga aaaccgggaa agcacagatg ctcgaataca aagacaacca gctgtgctca
    15181 ggtagcaaca aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac
    15241 acatccggaa aacctgtcgt tgtggctctg gacaaactta aaacctgagg agtaaaccat
    15301 gcgtttctct gcacattatt aatatcaaag gtaaatacag ccccagccat ttttgccgga
    15361 aagccgctta atgcgtggct cgccagtgaa aggcttctca gacaataaac ctcattcatt
    15421 tcacccgtca ggccgcgtct tctccgggag acgtggcttt tttcatttat tacaccagta
    15481 aatcttaacc accgataagg agcaaagtat gcgattagca agtcgttttg gttatgctgc
    15541 aaaccagata cgtcgtgacc gtccactgac acatgaagag ctgatgcacc atgtgccggg
    15601 gattttcggg gaagagaaac acacgtcccg aagccagaac tatacgtaca tccccaccat
    15661 caccgtactg gaaagcctgc agcgggaagg ctttcagcca ttcttcgcct gccagacccg
    15721 tgtgcgcgac ccgggccgcc ggggatacac caaacacatg ctgcgtctgc ggcgggccgg
    15781 agagataaac ggagagcatg tccctgaaat tattctgctc aactctcatg acggtacctc
    15841 cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacggct gcgtctgtgg
    15901 tcagtctctg ggggaagtgc gtgttccaca ccggggagac gtagtggaga aagttattga
    15961 aggggcttac gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg atgccatgca
    16021 gtcgctggtc ctgccgccac cggcacgcca ggcgctggca caggcggcac tgacttaccg
    16081 ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccac gacgccggga
    16141 ggattacggt aaggacctgt ggagtgctta tcagaccatc caggagaata tgctgaaagg
    16201 cgggatttcc ggtcgcagtg caaaaggaaa acgtatccat acccgggcca ttcacagcat
    16261 tgataccgac attaagctca accgtgcatt gtgggtgatg gcagaaacga tgctggagag
    16321 cctgcgctga tgcaatctgt ccctgaaggc gtgattcagg caccagaatc acccgacgcc
    16381 ccggagagct cagtctgtgc agactgagta caggcaatgg catctggcca tccgtctgtg
    16441 accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt catccctgac
    16501 attaccgggt ctcacggtca gtgatgaacg tgatgttgtc agcccccggc ctttactccc
    16561 tctcatttat tcacattaca aggatttcat acatgaaaac attgtctcag aacaccacct
    16621 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccaccata gtccctgatg
    16681 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtgaccctgg
    16741 agccccgggt cttcggctgg atggaccgtc tgtatgaaga ctactgcggt ggtatctgga
    16801 atctgtacac cctgaacaac ggtggcgcat ttatggcacc cgaaccggat gacgatgatg
    16861 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
    16921 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca cgcctgccgt acggagtgtt
    16981 atgccatgac ggttcattat taccgtctgc gggattacgc cctgcaacat ccggaatacg
    17041 acgccattat gcgcatcatt gactgaaagg gggcaggaac aatgcaacag atttcctttc
    17101 tgcccggaga gatgacgccc ggcgagcgca gtctcattct gcgggccctg aaaaccctgg
    17161 accgtcatct tcatgaaccc ggtgtggcct tcacctccac ccgtgcggca cgggaatggc
    17221 tgattctgaa catggcggga ctggagcgtg aagaattccg ggtgctatat ctgaataacc
    17281 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgc acggaagtcc
    17341 atccccggga agtgattaaa tgcgccctgt atcacaatgc cgcagccgtg gtgctggcac
    17401 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
    17461 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
    17521 gccaggtttt ctcctttgcg gaacacggcc tgctttaacc cgtcaccgcc acaatcacat
    17581 cacacctgtt ttcactttta tcttctgtct tcaaaggtat cctattatga aaattatcac
    17641 ccgtggtgaa gccatgcgta ttcaccaaca acatccgaca tcccgtcttt ttccgttctg
    17701 taccggtaaa taccgctggc acggcagtgc tgaagcgtat accggtcgtg aagtgcagga
    17761 tattcccggt gtgctggccg tgtttgctga acgccgtaag gacagttttg gcccgtatgt
    17821 ccgactgatg agcgtcaccc tgaactgagt gggaattctg atgagcagaa ttatcgccac
    17881 caccggacca ttcttaaccg attttctgta gggattttat cgtgtcagac acactccccg
    17941 ggacaacact tcccgacgac aatcacgacc gcccctggtg ggggctaccc tgcaccgtga
    18001 cgccctgttt cggcgcccgt ctggtgcagg agggtaaccg gttgcattac cttgccgacc
    18061 gtgccggtat cagaggcctg ttcagcgatg cagatgcgta ccacctggac caggcctttc
    18121 cgttgctgat gaaacaactg gagctcatgc tcaccagtgg tgaactcagc ccccgctatc
    18181 agcataccgt cacgctgtat gcgaaaggac tgacctgcga agccgatacc ctgggctcct
    18241 gtggttacgt ttatctggct gtttatccga cgcccgaaat gaaaaattaa ctctccagaa
    18301 tagccttctg ctacggcctg gtgttttcac cacgcaactt tcccattttt atatctgcat
    18361 atcaggaaaa tcttcagtat gaaaacatta cctgtattac ccgggcaggc ggccagttct
    18421 cgcccgtctc ctgttgaaat ctggcagata ctgctgtccc gactgctgga ccagcactac
    18481 ggcctcacac tgaatgacac accgtttgcc gatgaacgtg tgattgagca gcatattgag
    18541 gcaggcattt cactgtgtga tgcggtgaac tttctcgtgg aaaaatacgc gctggtgcgt
    18601 accgaccagc cgggattcag cgcctgtacc cgctctcagt taataaacag catcgatatc
    18661 ctccgggctc gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac
    18721 attaccctgg gtaagtatcc ggaggcgaaa tgatgacact ggaagccgac agcgttaacg
    18781 tacaggcgct ggatatgggg cacattgtcg ttgacattga tggtgttaat atcactgaac
    18841 tgattaataa ggccgctgaa aacggttatt cactccgcgt ggtggatggc cgtgactcta
    18901 ccgaaacacc ggcaacttat gccagccctc accagttgct gtaagacaat gcagtgatac
    18961 agggtatagt gccattaacc acagaaacag cactgttgtc ctgtattaag caggcaagag
    19021 aagatgtcct taccctgcgc catctgcagc ttctgcacca gaaccggtga atctcactca
    19081 acaggcttca tttcccctga cgccagcctg aatacagctg gcgttttcat ttataaacag
    19141 aaaggaaaac cgataataat ggaatggacg ccccgaccat gaagcaacgg taggatatta
    19201 ctgataaacc ttccagtgga ggtgcgttat ggccgtttta acgattagca ttgacctgac
    19261 aaaaaatgaa ttccaaatcc acggtctggg taggaacaga aaaatctaag ctcagaaaac
    19321 ggattaaagt aagcgtcaac ggagcaccgt attgacgctt atttattggt gagaactacg
    19381 ttccatggca ggagttcgtc aacacggttg gagggccatt ccggcagtat gctcagaata
    19441 tggcgcagat acgcttccgg atcgataccg ttcagacggc aggtgcctgc ttttccggca
    19501 acaagcagga atctcagcgc tgaagcagga attagaggta aaaacgccct accgggccat
    19561 gaatcacccg gtcattggag tagtaaccaa agcagattta gccagcatgg aacaaatatc
    19621 tttggtgaaa agttggttac gggaagctgg agcgcacaac gtgttagtaa ccagtgcagt
    19681 taataacaat ggagttacag aactttttgc cttgctgcat acagaagaag gctgttgtta
    19741 atttcgattg gttctcagat gagaatgatg acggtgatac acagataaga atcaatgagc
    19801 aatgagggtt gccgggcaac cctcattgaa taaaacggga attacttcgc ttcgccgttt
    19861 ttcatttcgc ccatagcttt cagctttttg gagatatcgc ggcgttcttt ggagagctcg
    19921 gcatttttga tgatgtaatc gtcaacgcga tcttcatagt cacctttcat gctggcgatg
    19981 atgccctgga ttgcttcaac gctcatccct ggcttgatgt aatcgctcag gttgtccagc
    20041 aacaggacgc gcttctggtt gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc
    20101 agtttgttct tacgacggaa cagacgaaca aattccagta cgtcctggaa tgaaggcttg
    20161 gtagtttcca tttttatacc cctgataatg tgagagtcgg attcgtttaa gcaaccgcta
    20221 ttcgttaggg ccaacattac tcatagctgc cgcagatgac aatgctttta tcccttttca
    20281 ctatcatacc ctttatcctt gctgaatcga agcagcagca agatgattct gaagttcagg
    20341 aattatttgc gcaaggcccg gcaaatcaga ttcgatagca actcaagctg atgtgccgtt
    20401 tccatgttca gccacacata accatagatt ggtgtttcca caaccttcag gtcatggtgg
    20461 ttatcgagca actgacgcag ctcttccaca gcatcgttca atttttccgt attggcaaaa
    20521 accggctgcg gattaccttc gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc
    20581 atgtgctggg tatcacgaag tttttgcgcg ttcaataaca cgaaatggct ggggcgcgtg
    20641 gcccagtatg cattgatttg caactccagc atacaaacca gattgcgatt aatggtctgg
    20701 ataccttcat atatcgattt gggaatacgg gtttctttgc tggcgggcgc aatcagccca
    20761 cgcattttca cggcatcggt caggagtttt tgtagatggc tttccaaacg tgggcgttcg
    20821 agtaagttcg gtgagaatgc agattgatag acccgattat actcggtcag acttttcgcc
    20881 agttgaatgc gccagtggat gaacgcccgt tgtggccaga taccggtaaa caacattgcc
    20941 agtaaagagc cgaggatcac atcgccgctt cgccataacg ccgtatcaat ttcacctgtc
    21001 ggggaaccca caacaattgc cagcgtcacc ccaatcaata atccttgata cggtttcttg
    21061 cccagcgcca gccaaccgca aagaaacatc gccgccgcgc accagactaa catcagcggt
    21121 aacgaaatta actccagttg cagagcgata agacctaaaa tcgaacctaa caccgtaccg
    21181 ccaatacgtt cgaaggcacg agggacaacg ttcccccaga acgagattgg ccccataatc
    21241 accaccatgg tgaccagcgg ccaggtgctt tccgggatag taaacaggcg gataatgaga
    21301 aaagtgagca ggaatgccag cgcgacccga gtaccatgca caatgcggta atggcggtat
    21361 acccggattt caaacgggct taatgactta tcggcacgca cgcagaaact ccgtaatgaa
    21421 gattaaaagc gctcataaca catctgtcgg ccagaaggcg cgcctctgag ttatccgacg
    21481 gacataccgg gaacagtgta atttcccggc aggagcgaaa aatttgaaag gcgcatctta
    21541 ctctttccgc tttcaaaaaa agagtggtct tcgcgttaac acaccgccct gagatgaatt
    21601 agtgatgttt tggctgcacc gcaacataca tttcgatatc ccagtaccca tcttccgcgc
    21661 cattgttcaa gtaaacctcg aagcagggct ttggtaacat ttcataagca ttgtcctgca
    21721 agaggctatt aaagaactga taccagggtt tagcaaaatc atcaccgact acacgagcca
    21781 ccgccaccgc atactgacca cctgaaattt ctgtcagaat gacgccctca ctgttttcgg
    21841 ggagcgtaaa gttattcggt accgtcacga cggtgtcgca gcgtaatttt tcggcgggtg
    21901 tttcatctgg attgtcgtaa tagacagcaa cccactcctt cggcacaata tttttgctat
    21961 ctatccacat catcaactgc tcaaaacctt tctttaccgt ctgctcccac gggccaacaa
    22021 gatggaaacc tgcaacggta cgtttatctt cctgcttaat ctcgtagttc atgacgcctc
    22081 cactaacact gtttttatat acagtatagt tgcaatatta aaaccacaag gaatgagtgt
    22141 tgattatgcg agcagactcg cactcctgcc agtctgctgc aaaagaaagg tcaggcctta
    22201 tggtggaaat aatcactcag gcgggaaaac atgctgcctt ccccgacaga ttccagggta
    22261 accagcggcc agtgcgccac ctgtttatca cggtcgtaaa gttcaatttc ccctacccgc
    22321 tgatgggcgc taattggcgc ggtgagttct ttaccatcaa gggtatattt ggctttgata
    22381 tgtggaattt cggctttcgg tagcaccatc cagaactctt gttccgttcc cagggcgata
    22441 ttttctttat cgccatacca gatgcgttcc gtaccgacct ttttcccacg gtgcaaaatt
    22501 tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt ttcttgcctc ttcctcacga
    22561 ccttttgcac tgtcagcacc cattaccact gcaatgaggc gacgctgccc gtctacagcc
    22621 gaagcaataa gattaaaccc ggcaccagaa gtatggcccg ttttcaggcc gtcaacattc
    22681 atggttttat cccacaataa cccgttacgg ttttgctggg tgataccgtt ccaggtgagg
    22741 cttttctcac tgtacatatg ataaaactcg ggctcgccgt ggatgatagc gcgagaaagc
    22801 acggctaaat cataagccga gctatgctgg ccaggtgcat ccagaccatg cactgtttca
    22861 aaatgcgtat ccttgagatg cagcttctcg gcatagttgt tcatcatttc aacaaactgc
    22921 cgttgcccac cggcaatata gtcagccaga gcaacacaag cgtcatttcc ggaatccaca
    22981 attaaaccac ggcttaaatc acgtaccgat acgcgatcgc cctctttcaa aaacatcagt
    23041 gaagaaccga caaacaccgg attatctttc gcccacgcat cacgccccac ggtgacaata
    23101 tcgtctggcg taatgcgatg actatcgata gcgcgatcca cgacatagcc cgtcatcagc
    23161 tttgtcaggc tggcgggatt gcgctgttga tgctcattac ccgcggtgag aatctgaccg
    23221 gtggtgtaat ccatcaatac ccaggacccg gcatgaatct ctggaggctg aggtgaaaaa
    23281 ggaatgtttt ccgccgcaaa accagacgat aaattaaaaa cgaacaaaga agcagcaata
    23341 ataagacggc gtttcaacag caaaccctca ggtgtttcaa atagctgttc tttttacgga
    23401 aatacttatg aactggctgg aataaagtgc aagaaaatgt gactactatc tcatttttat
    23461 ctgacatgat ctgttgccac tcgctgccaa attgtcgcgc taaagctgat tagcacggtg
    23521 atatttgata ctctggcaga cagcagaaat aacggattta acctaatgat gaatgacggt
    23581 aagcaacaat ctaccttttt gtttcacgat tacgaaacct ttggaacgca ccccgcgtta
    23641 gatcgccctg cacagttcgc agccattcgc actgataacg aattcaatgt catcggcgaa
    23701 cccgaagtct tttactgcaa gcccgcggat gactatttac cccagcctgg agcagtatta
    23761 attaccggta ttaccccgca ggaggcacgg gcgaaaggag aaaacgaagc cgcgtttgcc
    23821 gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta caacaatgtg
    23881 cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga tccttacgcc
    23941 tggagctggc agcatgataa ctcgcgctgg gatttactgg atgttatgcg tgcctgttat
    24001 gccctgcgcc cggaaggaat aaactggcct gaaaatgatg acggtctacc gagctttcgc
    24061 cttgagcatt taaccaaagc gaatggtatt gaacatagca acgcccacga tgcgatggct
    24121 gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa cgcgtcagcc acgcctgttt
    24181 gattatctct ttacccatcg taataaacac aaactgatgg cgttgattga tgttccgcag
    24241 atgaaacccc tggttcacgt ttccggaatg tttggggcat ggcgcggcaa taccacctgg
    24301 gtggcaccgc tggcgtggca tcctgaaaat cgcaatgccg taattatggt ggatttggca
    24361 ggagacattt cgccattact ggagctggat agcgacacat tgcgcgagcg tttatatacc
    24421 gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtgca tatcaataaa
    24481 tgtccggtac tggcccaggc gaatacgcta cgcccggaag atgccgaccg actgggaatt
    24541 aatcgtcagc attgcctcga taacctaaaa attctgcgtg aaaatccgca agtgcgtgaa
    24601 aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc cttcagagaa cgtggatgca
    24661 cagctttata atggcttttt cagtgacgcc gatcgtgcag caatgaaaat tgtgctggaa
    24721 accgagccgc gtaatttacc ggcactggat atcacttttg ttgataaacg aattgaaaaa
    24781 ctgttgttca attatcgagc acgcaacttc ccggggacgc tggattatgc cgagcagcaa
    24841 cgctggctgg agcaccgccg ccaggtcttc acgccagagt ttttgcaggg ctacgctgat
    24901 gaattgcaga tgctggcaca acagtatgcc gatgacaaag agaaagtggc gctgttaaaa
    24961 gcgctttggc agtacgcgga agagatcgtc tgaaacaaaa aagccggagc gacaaactcc
    25021 ggctttttga gatttaccgg atgattatgt catccggttt gtaagattac gctacgtctt
    25081 cgtactgcgg aaccgggtta cggaaactct tggttacgca agccaggtaa atcagaccga
    25141 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
    25201 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgttt
    25261 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaacg aaggtgaacg
    25321 ccaccaacgc accaaagtta atcagcgccg tagccattac caggtcgaag ttgattgcca
    25381 gcagggcaat cgcgccaacc agaatgatgt tcatcgccgg agtacgccat ttcgggtgta
    25441 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
    25501 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
    25561 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
    25621 gatctttaaa acgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
    25681 agatcatgcc gccgatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
    25741 ctttggtttc ttccgacagg ttgctgatgc cgtcaaaacc ggtaaaggag aagcacagga
    25801 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgagtgc
    25861 tcgccagcgt accagcgcct tcaccttcaa atacgccata aaccaccatg cccagaatca
    25921 ccgcgatcag cactatctgc aacacgacga ttacagtgtt gaagttcgct acggatttca
    25981 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
    26041 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
    26101 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc ggactaatgg
    26161 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
    26221 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
    26281 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
    26341 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
    26401 tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa
    26461 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
    26521 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
    26581 aaccgacgct aattaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta
    26641 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttaaatggc
    26701 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
    26761 atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa tccagacggg
    26821 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
    26881 ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca gctccagctt
    26941 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
    27001 agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg
    27061 gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
    27121 aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
    27181 aacgataata tccgctcgcc cctgagtcag ggcatcccat gaaccaccga gtacgccatt
    27241 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag
    27301 cagagaaaaa gggaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
    27361 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
    27421 taataacatt tttccggtac gggtgaattt agcgcggtgt ccgctacgat caagcaattg
    27481 aatattaagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
    27541 gagtttggct gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa
    27601 cacatccagc agtggtttca tacacgcccc cttgcagtgt atgcggcgtc ctgctggcgt
    27661 gatcgtttac tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
    27721 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
    27781 ctgcggaggt tccagcccca ataaacgggc catctgcgga tagaaaacat tacgcgcagg
    27841 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
    27901 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
    27961 ggtttttccg gcaaagaagc gtccgggatg acgtcccggt ccgaccaggc ccgcaagacg
    28021 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
    28081 tcgtccactg ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc
    28141 gccatagaca gatgtcgagc tggtaaaaat aatgcgggga atacgatggg ccagcgcgct
    28201 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
    28261 cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt cagaatcgca
    28321 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
    28381 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
    28441 cgccagcggc atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct
    28501 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
    28561 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcgtcggtaa
    28621 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaagcct gacaaattat
    28681 gtttcagggg ttctggcttc cccttacctt caaatggtgt tctgcgtgta tctttgataa
    28741 tttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
    28801 atgattcctc agaccagatt agtttcactc aataatgtcc ttttccgttc ctttgcctga
    28861 tttcaggcta tcgattgagt ccatcaatct ccgggcgtta gcgggggaac gcagtagata
    28921 agccgtctct tccagcgagt tgtattcttc gagtgacatc agaacacaag cctctccatt
    28981 ctgacgagta atgaggatcg gggcatgatc ttcaacggct ttcatcattg ttgccgacaa
    29041 attctgacgc gcttcgctgt agctaattgt acgcatgtaa atctcctctt ttgtacagct
    29101 cattgtacaa tgatgggcgt taattaacta tttattaatt agtttgtaga tcaagatatt
    29161 gtcagcgaga cgaaaaatca gactttgctc tttttggtgc tgtcaagtta gaggacagtc
    29221 ctcttagccc cctcctttcc ccgctcattc attaaacaaa tccatcgcca taaaatatat
    29281 aaaaaacccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc agttgaataa
    29341 acattcacag agatttttat gacacgcgtt caatttaaac accaccatca tcaccatcat
    29401 cctgactagt ctttcaggcg atgtgtgctg gaagacattc agatcttcca gtggtgcatg
    29461 aacgcatgag aaagcccccg gaagatcatc ttccgggggc ttttttattg cgcagttgat
    29521 aacggttcag acaggtttaa agaggaataa gaaaatgaca gacaacactc gtttacgcat
    29581 agctatgcag aaatccggcc gtttaagtga tgactcacgc gaattactgg cgcgctgtgg
    29641 cattaaaatc aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca tgccgattga
    29701 tattctgcgc gtgcgtgacg acgacattcc cggtctggtg atggacggcg tggtagacct
    29761 tgggattatc ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg cccagggtga
    29821 agatccacgc tactttaccc tgcgtcgact ggatttcggc ggctgccgcc tttcgctggc
    29881 aacgccggtt gatgaagcct gggacggccc gctctcctta aacggtaaac gtatcgccac
    29941 ctcttatcct cacctgctca agcgttatct cgaccagaaa ggtatctctt ttaaatcctg
    30001 cttactgaac ggttctgtcg aagttgcgcc gcgcgccgga ctggcggatg cgatttgcga
    30061 tctggtttcc accggtgcca cgctggaagc taacggcctg cgcgaagtcg aagttatcta
    30121 ccgctcgaaa gcctgcctga tccaacgcga tggcgaaatg gaagaatcca aacagcaact
    30181 gatcgacaaa ctgctgacgc gtattcaggg cgtgatccag gcgcgcgaat caaaatacat
    30241 catgatgcac gcaccgaccg accgactgga tgaagtcatc gccctgctgc caggtgccga
    30301 acgcccaacc attctaccgc tggcgggtga tcaacaacgc gtagcgatgc acatggtcag
    30361 cagcgaaacc ctgttctggg aaacgatgga aaaactgaaa gcgctgggtg ccagttcaat
    30421 cctggtcctg ccgattgaga agatgatgga gtgatcgcca tgagctttaa cacaatcatt
    30481 gactggaata gctgtactgc ggagcaacaa cgccagctgt taatgcgccc ggcgatctcc
    30541 gcctctgaaa gcattacccg caccgttaac gatattctcg ataacgtgaa aacgcgtggc
    30601 gatgaggccc tgtgtgaata cagcgcgaag tttgataaaa ccacggttac cgcgctgaag
    30661 gtgtctgctg atgagatcac cgccgccagc gaacgcctga gcgacgagct aaaacaggcg
    30721 atggcggtgg cagtaaagaa tattgaaacc ttccacactg cgcaaaaact gccgccggta
    30781 gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca cgcgcccggt ggcttcagtg
    30841 ggtttgtata ttcctggcgg ctccgccccg ctcttctcaa cggtattaat gctggcaact
    30901 ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct caccgccgcc gattgccgat
    30961 gagatccttt atgcggcgca gctgtgcggt gtgcgggacg tgtttaacgt cggcggcgca
    31021 caggccattg ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga caaaatcttc
    31081 gggccgggta acgcctttgt caccgaagca aaacgccagg taagccagcg tctggacggt
    31141 gcggcgatcg atatgcccgc aggtccgtcg gaagtgctgg tgattgctga cagcggcgct
    31201 acgccggatt tcgtggcttc tgatttgctt tctcaggctg aacacggccc ggactcacag
    31261 gtgattttac tgacccccga cgccgatatg gcgcgtcgcg ttgccgaggc cgtcgaacgc
    31321 caactggcag aactgccgcg tgccgaaacc gcccgccagg cactgaacgc cagccgcctg
    31381 atcgtgacta aagatttagc gcagtgcgtg gagatctcca accagtacgg cccggagcac
    31441 ctgatcattc agacccgcaa cgcccgcgaa ctggtcgatg gcatcaccag cgccggttcg
    31501 gtatttcttg gtgactggtc accggaatcg gcaggcgact acgcctccgg caccaaccac
    31561 gttctaccga cttacggcta caccgccacc tgttccagcc tcgggctggc agatttccag
    31621 aagcgcatga ccgtgcagga actctcgaaa gaaggttttt ccgtgctggc gtcgaccatt
    31681 gaaacactcg ccgccgccga gcgcctgacc gcccacaaaa atgccgttac tttgcgtgtt
    31741 aacgccctta aggagcaagc atgagcaccg tgactattac cgatttagcg cgtgaaaacg
    31801 tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg cggtaacggc gacgtctggc
    31861 tgaacgccaa cgaatacccc acagccgtgg agtttcagct cactcaacaa acgctcaacc
    31921 gctatccgga atgtcagcca aaagcggtga ttgaaaatta cgcgcaatat gcaggcgtaa
    31981 aaccggaaca ggtgctggtc agccgtggcg cggacgaagg tattgaactg ctgattcgcg
    32041 ctttttgcga accgggtaaa gacgccatcc tctactgccc gccaacgtac ggcatgtaca
    32101 gcgtcagcgc tgaaaccatt ggcgtcgagt gccacacagt gccgacgctg gacaactggc
    32161 aactggactt acagggcatt tccgacaagc tggacggcgt aaaagtggtc tatgtttgca
    32221 gccccaacaa ccccaccggg caactgatca acccgcagga ttttcgcacc ctgctggagt
    32281 taacccgcgg taaggcgatt gtggttgccg atgaagccta tatcgagttt tgcccgcagg
    32341 catcgctggc tggctggctg gcggaatatc cgcacctggc tattttgcgc acactgtcga
    32401 aagcttttgc tctggcgggg cttcgttgcg gatttacgct ggcaaacgaa gaagtcatca
    32461 acctgctgat gaaagtgatc gccccctacc cgctctcgac accggttgcc gacattgcag
    32521 ctcaggcgtt aagcccgcag ggaatcgtcg ccatgcgcga acgagtggcg caaattatta
    32581 cagaacgtga atacctgatt accacactga aagagatccc ctgcgtggag cagttttttg
    32641 actctgaaac caactacatt ttggcacgct ttaaagcctc cagcgcagtg tttaaatctt
    32701 tgtgggatca gggcattatc ttacgtgatc agaataaaca accctcttta agcggctgcc
    32761 tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc ttacgtgcgg
    32821 agcaagtttg atgagtcaga agtatctttt tatcgatcgc gatggaaccc tgattagcga
    32881 accgccgagt gattttcagg tggaccgttt tgacaaactc gcctttgaac cgggcgtgat
    32941 cccggagctg ctgaggctgc aaaaagcggg ctacaagctg gtgatgatca ctaatcagga
    33001 tggtcttgga acacaaagtt tcccgcaggt ggatttcgaa ggcccgcaca acctgatgat
    33061 gcagatcttc acctcgcaag gcgtgcagtt tgatgaagtg ctgatttgtc cgcacctgcc
    33121 cgccgatgag tgcgactgcc gtaagccgaa agtaaaactg gtggagcgtt atctggctga
    33181 gcaagcgatg gatcgcgcca acagttatgt gattggcgat cgcgcgaccg acattcaact
    33241 agcggaaaac atgggcatta atggtttacg ctacgaccgt gaaatcctga actggccgat
    33301 gattggtgag caactcacta aacgagaccg ttacgcccat gtagtgcgca acaccaaaga
    33361 gacgcaaatt gacgtccagg tgtggctgga tcgcgaaggt ggcagcaaga ttaacaccgg
    33421 cgttggcttc tttgatcaca tgctggatca gatcgccacc cacggcggtt tccgtatgga
    33481 aatcaacgtc aaaggcgacc tctatatcga cgatcaccac accgtcgaag ataccggcct
    33541 ggcgctgggg gaagcgttaa aaattgccct ctgcgataaa cgcggtattt gccgctttgg
    33601 ttttgtgctg ccgatggacg aatgccttgc ccgctgcgcg ctggatatct ctggtcgccc
    33661 gcacctggaa tataaagctg aatttaccta ccagcgcgtg ggcgatctca gcaccgagat
    33721 gatcgagcac ttcttccgtt cgctttcata caccatgggc gtgacgctac acctgaaaac
    33781 caaaggtaaa aacgatcacc accgtgtaga gagcctgttc aaagcctttg gtcgtaccct
    33841 gcgccaggcc atccgcgtcg aaggcgacac cctgccctcg tcgaaaggag tgctgtaatg
    33901 aacgtggtga tccttgatac tggctgcgcc aacctgaact ctgtgaagtc tgccattgcg
    33961 cgccacggtt atgaacccaa agtcagccgt gacccggacg ttgtgttgct ggccgataaa
    34021 ctgtttttac ccggcgtcgg caccgcacaa gcagcgatgg atcaggtgcg tgagcgcgag
    34081 ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctgg gcatctgctt agggatgcaa
    34141 ctcctgggac gacgcagcga agagagcaat ggcgtcgact tgctgggcat catcgacgaa
    34201 gacgtaccga aaatgaccga ctttggtctg ccactgccac atatgggctg gaatcgcgtt
    34261 tacccgcagg caggcaaccg cctgtttcag gggattgaag acggcgcgta cttttacttt
    34321 gttcacagct acgcaatgcc ggtcaatccg tggaccattg cccagtgtaa ttacggcgaa
    34381 ccgttcaccg cggcggtaca aaaagataac ttctacggcg tgcagttcca tccggagcgt
    34441 tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg agatgtgatg attattccgg
    34501 cattagattt aatcgacggc accgtggtgc gtctccatca gggcgattac ggcaaacaac
    34561 gcgattacgg taacgacccg ctgccgcgct tacaggatta cgccgcacaa ggtgccgaag
    34621 tgttgcatct ggtggatctg accggggcca aagatccggc taagcgtcaa atcccgctga
    34681 ttaaaaccct ggtcgcgggc gttaacgtcc cggtgcaggt tggcggcggt gtgcgtagtg
    34741 aagaagacgt ggcggcgtta ctggaagctg gcgttgcgcg cgtggtggtc ggttccaccg
    34801 cagtgaaatc accggacgta gtgaaaggct ggtttgaacg tttcggtgcc gatgcgctgg
    34861 tgctggcact ggatgtccgt attgacgagc aaggcaacaa gcaagtggca gtcagcggct
    34921 ggcaagagaa ctcgggcgtt tcactggaac aactggtgga aacctatctg cccgtcggcc
    34981 tgaaacatgt gttgtgtacc gatatctcgc gcgacggtac gctggcggga tctaacgtct
    35041 ctttatatga agaagtgtgt gccagatatc cgcaagtggc gtttcagtcc tccggcggca
    35101 ttggcgacat taacgatgtt gctgccctac gtggcactgg cgtgcgcggc gtaatagttg
    35161 gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc catcgcatgc tggcaaaacg
    35221 cataatcccg tgtctcgacg ttcgtgatgg tcaggtggtg aaaggcgtac agtttcgcaa
    35281 ccatgaaatc attggcgata tcgtgccact ggcaaaacgc tacgccgaag aaggtgcaga
    35341 tgaactggtg ttctacgata tcaccgcttc cagcgatggc cgtgtggtag ataaaagctg
    35401 ggtatctcgc gtggcggaag tgatcgacat tccgttttgt gtggcgggtg ggattaagtc
    35461 tctggaagat gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca tcaactctcc
    35521 tgcgctggcg gacccgacgt taattactcg cctggcggat cgctttggcg tgcagtgtat
    35581 tgtggtcggt attgatacct ggtacgacgc cgaaaccggt aaatatcatg tgaatcaata
    35641 taccggcgat gaaagccgca cccgcgtcac tcaatgggaa acgctcgact gggtagagga
    35701 ggtgcaaaaa cgcggcgccg gagaaatcgt cctcaacatg atgaatcagg acggcgtgcg
    35761 taacggttac gacctcaagc agctgaaaaa agtgcgtgaa gtttgccacg tcccgctgat
    35821 tgcctccggt ggcgcgggca ccatggaaca cttcctggaa gccttccgcg atgccgacgt
    35881 tgacggtgcg ctggcagctt ccgtattcca caaacaaata atcaatattg gcgaattaaa
    35941 agcgtacctg gcaacacagg gcgtggagat caggatatgt taacagaaca acaacgtcgc
    36001 gaactggact gggaaaaaac cgacggtctg atgccggtga ttgtgcaaca cgcggtatcc
    36061 ggcgaagtgt taatgctggg ctacatgacc ccggaagcct tagacagaac catcgaaagc
    36121 ggtaaagtta ccttcttctc gcgcactaaa cagcgactgt ggaccaaagg cgaaacctcg
    36181 ggcaatttcc tcaatgtggt gagcattgcg ccggactgcg acaacgacac gttactggtg
    36241 ctggcgaatc ccatcgggcc aacctgccac aaaggcacca gcagctgctt cggcgacacc
    36301 gctcaccagt ggctgttcct gtatcaactg gaacaactgc tcgcggaacg taaatccgcc
    36361 gacccggaaa cctcttacac tgccaaactg tatgccagcg gcaccaaacg cattgcgcag
    36421 aaagtgggcg aagaaggcgt ggaaaccgca ctggcagcaa cggtacatga ccgctttgaa
    36481 ctgaccaacg aagcgtctga tttgatgtat cacctgctgg tgctgttgca ggatcaggat
    36541 ctggatttaa cgacggtaat tgagaacctg cgtaaacggc atcagtgagt tgcgtgctga
    36601 gcaggtgtga tgttgttgtc acacccggag ccattgcccg atgcgcaagc ttatcgtgcc
    36661 tacacattat ctcaacacac gtaggccgga taagatgcgc cagcatcgca tccggcaaaa
    36721 aaaacgggca tggtgtcacc accctgcccg tttctcttaa atgcacaata atattatttc
    36781 gcgttgtaat tacgcagcgc gttacgccca agcacaatcc ccgcgccaac catgccgccc
    36841 agcagcacag ccagaatcaa ggtaattgct ttcttcgggc tatcgcgacg aataggtaac
    36901 gtcggtttca ttacatagcg gtaagcatga atatcaagtt tatcaacgtc aagattgtca
    36961 atatccagca ggttttgacg agtctgatag tagtttgatg agaacaccaa cggacgggtc
    37021 gcttcgtgct taatcatcga ctccagcgct tcgctcccca aaagaaacat agtatcctgg
    37081 gttacgtcct gtgtctgttg aatctgcggc tttgtcacct gcgcctgatt cgcatactgc
    37141 aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcgac cacttcctgg
    37201 gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcctt gagatccttt
    37261 tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa cttcatttgt
    37321 gcgccctctg cagtttgccc aacataagaa acagtcaatg gtaattgctg gttcttaaca
    37381 gaaggttcga tggtaagttt ttctggctct tcctgattat ccagcgtttc tgctaatgca
    37441 gagaaggcag aactgaagcg accaattaac gtctcctgca aatccgatac tttcggtgca
    37501 gcctgaccat agataacatt catggcattg ttatagccag caatttgccc cacgtcgggc
    37561 tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata accaatagcc
    37621 agggcaatag ccacaatgac ggaaatgata attgtcatct tgccacgcca caactgcact
    37681 agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac attattattt
    37741 tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt ttagcgataa
    37801 tccccgtgaa tgctatagga taagtgataa aaataatgaa ttattgcgga ggtaacaaaa
    37861 gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa agagatcgcg
    37921 ggtgtagacc ttatctgcca catccttaag ctcttctgcc atacggttgg agataatgac
    37981 gtcggcttgt tgtttgaagg tggcgagatc acgttccagg cgagagttga agaatgagtc
    38041 ttctttcatc accggctcgt agatgatcac ttcaacgcct ttcgccttga tacgcttcat
    38101 aatcccctga atagaagacg cacggaagtt atctgacccg ctcttcataa tcagacgata
    38161 aatacccacc acttgcggtt tgcgtgacaa aatggcatcg gcaataaaat ctttacgcgt
    38221 gcggtttgca tcgacaattg ccgagatcag gttattcggc acagactggt agttcgccag
    38281 taactgcttg gtatctttcg gcagacaata accaccataa ccaaacgacg gattgttgta
    38341 atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag tattcagacc
    38401 taaactttct gcatagctat ccagttcatt aaagtatgct acgcgcatcg ccagataggt
    38461 attagcgaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg tcgggatatt
    38521 ttgcttaatc gcgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt ctgaacgctc
    38581 accgatgaca atacgtgacg gatggagatt atcgtaaagg gctttacctt cgcggaggaa
    38641 ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctg cggtaaaacc
    38701 aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa ctacgtcttt
    38761 aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat agtcggttgg
    38821 agtggcgatg atgacataat cagcatcccg gtaggcttca tttttatcta atgtggcatt
    38881 aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa taggagatat
    38941 ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa cctcatgatt
    39001 ttgtgcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa tggtgatttt
    39061 catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc atcgtgataa
    39121 gatgctaaaa gttttaacgc tacgttgtca gatggtcaac gcaacgaaaa acaattcaga
    39181 ttttctttat aagaatagct gatgtttatt aagttaatat taagcagtga aatttagtct
    39241 atagatattt ggcaaaaaaa agcccggtgc aatatacgcc gggcctcaat tttattgttg
    39301 gttaaatcag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
    39361 taagtatgcg ccccgaaata gtcacgctgt gcctggatca ggttcgcagg cagaacagcg
    39421 gcacggtagc tgtcataata ggcaaccgca gcggcgaagg tcggaaccgg gataccgttc
    39481 tgtactgcat aagcgacgac atcgcgcagc gcctgctggt agtcatcggc aatttgcttg
    39541 aagtacgggg ccagcagcag gttagcgatc tgcggatttt cggcataagc atcggtgatt
    39601 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcgccg
    39661 tagttcagat cccagttgta ttcttcagac gcagcacgca gctgagagaa gccctgagcg
    39721 taagaaacga ttttgcccag atacaacgca cggcgaactt tttcgatgaa ctcacctttg
    39781 tcgcctgctg gctgcgcttg cgggccagag agaactttag atgcggcaac gcgctgatct
    39841 ttcagagaag agatataacg tgcaaacaca gactcggtaa tcagcgacag cggttcgccg
    39901 agatccagcg cgctctggct ggtccattta ccggtgcctt tgtttgctgc ttcatccaga
    39961 atcacatcaa ccaggtagtt accgtcttca tcttttttgg tgaagatatc tttagtgatg
    40021 tcgatcaggt agctgctaag ttcaccgtta ttccactcgg tgaaagtctg ggccagttct
    40081 tcattagaga gattcaggcc acctttgagc agagaatacg cttcagcaat gagctgcata
    40141 tctccgtatt caataccatt gtgaaccatc ttcacataat ggcctgcgcc gtcggcacca
    40201 atataagtaa cgcatggctc gccgtcttct gccacggcag cgattttact caggataggc
    40261 gcaaccagct cataagcctc tttctgcccg ccaggcataa tagaaggacc tttcagcgcg
    40321 ccctcttcac caccggagac accggtacca atgaagttaa agccttcggc agaaagctca
    40381 cggttacgac gaatggtgtc ctggaagaag gtattaccac catcaatgat gatgtcacct
    40441 ttatcgaggt atggcttgag ggaatcaata gcagcatccg tgcctgcacc tgctttcacc
    40501 attaacagga tgcgacgagg cgtttccaaa gattcaacaa actctttcac cgtatagtaa
    40561 ggaaccagtt ttttgcctgg attttcggca atcacttctt cggtcttttc acgggaacgg
    40621 ttgaaaatag agacggtata accacggctt tcgatattga gcgcaaggtt gcgccccatc
    40681 actgccatac cgacgacgcc gatctgttgc tttgacattg tttactcct
//
LOCUS       DAZFRE010000032        40497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFRE010000032 DAZFRE010000000
VERSION     DAZFRE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.032"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            143..385
                     /gene="yjbE"
                     /locus_tag="ACHKUI_002069"
     CDS             143..385
                     /gene="yjbE"
                     /locus_tag="ACHKUI_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6598259.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            500..1138
                     /gene="yjbF"
                     /locus_tag="ACHKUI_002070"
     CDS             500..1138
                     /gene="yjbF"
                     /locus_tag="ACHKUI_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6598260.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1135..1872
                     /gene="yjbG"
                     /locus_tag="ACHKUI_002071"
     CDS             1135..1872
                     /gene="yjbG"
                     /locus_tag="ACHKUI_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6598261.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVMLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1872..3968
                     /gene="yjbH"
                     /locus_tag="ACHKUI_002072"
     CDS             1872..3968
                     /gene="yjbH"
                     /locus_tag="ACHKUI_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6598262.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLVQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4015..4293)
                     /locus_tag="ACHKUI_002073"
     CDS             complement(4015..4293)
                     /locus_tag="ACHKUI_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598263.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4508..4918
                     /gene="psiE"
                     /locus_tag="ACHKUI_002074"
     CDS             4508..4918
                     /gene="psiE"
                     /locus_tag="ACHKUI_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6598264.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5012..5902)
                     /gene="malG"
                     /locus_tag="ACHKUI_002075"
     CDS             complement(5012..5902)
                     /gene="malG"
                     /locus_tag="ACHKUI_002075"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6598265.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5917..7461)
                     /gene="malF"
                     /locus_tag="ACHKUI_002076"
     CDS             complement(5917..7461)
                     /gene="malF"
                     /locus_tag="ACHKUI_002076"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6598266.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7707..8897)
                     /gene="malE"
                     /locus_tag="ACHKUI_002077"
     CDS             complement(7707..8897)
                     /gene="malE"
                     /locus_tag="ACHKUI_002077"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6598267.1"
                     /translation="MKIKTGARILALSALTTMMFSASTLAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9262..10377
                     /gene="malK"
                     /locus_tag="ACHKUI_002078"
     CDS             9262..10377
                     /gene="malK"
                     /locus_tag="ACHKUI_002078"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6598268.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10449..11789
                     /gene="lamB"
                     /locus_tag="ACHKUI_002079"
     CDS             10449..11789
                     /gene="lamB"
                     /locus_tag="ACHKUI_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6598269.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            12203..13123
                     /gene="malM"
                     /locus_tag="ACHKUI_002080"
     CDS             12203..13123
                     /gene="malM"
                     /locus_tag="ACHKUI_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6598270.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13302..13799
                     /gene="ubiC"
                     /locus_tag="ACHKUI_002081"
     CDS             13302..13799
                     /gene="ubiC"
                     /locus_tag="ACHKUI_002081"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6598271.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13812..14684
                     /gene="ubiA"
                     /locus_tag="ACHKUI_002082"
     CDS             13812..14684
                     /gene="ubiA"
                     /locus_tag="ACHKUI_002082"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6598272.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14839..17262)
                     /gene="plsB"
                     /locus_tag="ACHKUI_002083"
     CDS             complement(14839..17262)
                     /gene="plsB"
                     /locus_tag="ACHKUI_002083"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6598273.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17433..17801
                     /gene="dgkA"
                     /locus_tag="ACHKUI_002084"
     CDS             17433..17801
                     /gene="dgkA"
                     /locus_tag="ACHKUI_002084"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6598274.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17911..18519
                     /gene="lexA"
                     /locus_tag="ACHKUI_002085"
     CDS             17911..18519
                     /gene="lexA"
                     /locus_tag="ACHKUI_002085"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6598275.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18592..19917
                     /gene="dinF"
                     /locus_tag="ACHKUI_002086"
     CDS             18592..19917
                     /gene="dinF"
                     /locus_tag="ACHKUI_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6598276.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            20033..20242
                     /gene="yjbJ"
                     /locus_tag="ACHKUI_002087"
     CDS             20033..20242
                     /gene="yjbJ"
                     /locus_tag="ACHKUI_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6598277.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20284..20799)
                     /gene="zur"
                     /locus_tag="ACHKUI_002088"
     CDS             complement(20284..20799)
                     /gene="zur"
                     /locus_tag="ACHKUI_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6598278.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20955..21950
                     /gene="dusA"
                     /locus_tag="ACHKUI_002089"
     CDS             20955..21950
                     /gene="dusA"
                     /locus_tag="ACHKUI_002089"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6598279.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            22084..22326
                     /gene="pspG"
                     /locus_tag="ACHKUI_002090"
     CDS             22084..22326
                     /gene="pspG"
                     /locus_tag="ACHKUI_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6598280.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(22492..23475)
                     /gene="qorA"
                     /locus_tag="ACHKUI_002091"
     CDS             complement(22492..23475)
                     /gene="qorA"
                     /locus_tag="ACHKUI_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6598281.1"
                     /translation="MAIRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23558..24973
                     /gene="dnaB"
                     /locus_tag="ACHKUI_002092"
     CDS             23558..24973
                     /gene="dnaB"
                     /locus_tag="ACHKUI_002092"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6598282.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            25026..26105
                     /gene="alr"
                     /locus_tag="ACHKUI_002093"
     CDS             25026..26105
                     /gene="alr"
                     /locus_tag="ACHKUI_002093"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6598283.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26358..27551
                     /gene="tyrB"
                     /locus_tag="ACHKUI_002094"
     CDS             26358..27551
                     /gene="tyrB"
                     /locus_tag="ACHKUI_002094"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6598284.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSSAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            27733..30477
                     /locus_tag="ACHKUI_002095"
     CDS             27733..30477
                     /locus_tag="ACHKUI_002095"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6598285.1"
                     /translation="MENMTSPGTLLSGDNATWLEDYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAIIQLINAWRTQGHLRAKLDPLGLNPPADVPSLLPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKCLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLTLRQDMAAGT
                     QPIDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELSEFTDGAYKPVVTDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQRECEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            30510..31664
                     /gene="odhB"
                     /locus_tag="ACHKUI_002096"
     CDS             30510..31664
                     /gene="odhB"
                     /locus_tag="ACHKUI_002096"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6598286.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            31676..33094
                     /gene="lpdA"
                     /locus_tag="ACHKUI_002097"
     CDS             31676..33094
                     /gene="lpdA"
                     /locus_tag="ACHKUI_002097"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6598287.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            33116..34285
                     /gene="sucC"
                     /locus_tag="ACHKUI_002098"
     CDS             33116..34285
                     /gene="sucC"
                     /locus_tag="ACHKUI_002098"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6598288.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFATFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            34298..35170
                     /gene="sucD"
                     /locus_tag="ACHKUI_002099"
     CDS             34298..35170
                     /gene="sucD"
                     /locus_tag="ACHKUI_002099"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6598289.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            35382..36881
                     /locus_tag="ACHKUI_002100"
     CDS             35382..36881
                     /locus_tag="ACHKUI_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFO6598290.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            36893..37966
                     /locus_tag="ACHKUI_002101"
     CDS             36893..37966
                     /locus_tag="ACHKUI_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO6598291.1"
                     /translation="MSLQITVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(37955..39313)
                     /locus_tag="ACHKUI_002102"
     CDS             complement(37955..39313)
                     /locus_tag="ACHKUI_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFO6598292.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(39306..>40497)
                     /locus_tag="ACHKUI_002103"
     CDS             complement(39306..>40497)
                     /locus_tag="ACHKUI_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6598293.1"
                     /translation="SKSPWRMRTLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVS
                     NFTFLKDKKEQLCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWAILLVITLIIYLL
                     FLLFIKYWRMRSHAQQLLTLANETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQ
                     ITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKH
                     IISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAP
                     SMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGF
                     APEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWG
                     EEHE"
BASE COUNT         9573 a        10055 c        10894 g         9975 t
ORIGIN      
        1 atatttgata ttcattccaa ttatcctaaa accccatcac taattccccg cgccgtaatt
       61 cgcatgcttt agttgtgtat actcgatccc gcccgaaatg tttttgggta aatctccatt
      121 cattcaatga agggaaattg ttatgaaaaa agttctgtat ggcatttttg ccatatctgc
      181 gcttgcggcg acttctgcgt gggctgcacc tgtacaggtg ggcgaagcgg caggatcggc
      241 agcaacgtcg gtttcggcgg ggagttcctc cgcgaccagc gtcagcaccg taagctcggc
      301 ggtgggtgtc gcgctcgcgg caaccggtgg cggtgatggt tctaataccg ggaccaccac
      361 aaccacgacc accagtaccc agtaataaag tatgtatccc caaaataatt cgagtcatcg
      421 catctgaagc ttgaagtatg aaggggatta accataacca cactccggtg tggttattct
      481 gcccctctgg agaagagtcg tgaagcgacc agcactcatt cttatctgcc tgctattaca
      541 ggcctgttca gccacgacta aagagctggg caattcactg tgggacagtc tgttcggcac
      601 gccaggcgta cagctgacgg acgatgatat tcaaaatatg ccctacgcca gccagtacat
      661 gcagcttaat ggcgggccgc agttatttgt ggtgctggcc ttcgctgaag acggacaaca
      721 aaaatgggtc actcaggatc aggccaccct tgtcacacaa catggtcgtc tggtgaagac
      781 tttgcttggc ggcgacaacc tgattgaagt gaataacctt gccgccgacc cgctgattaa
      841 acccgcacaa attgttgatg gtgcaacatg gacgcgcacg atgggctgga ccgagtacca
      901 gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa
      961 agtcggcagc gatgaaactc cggttcgcgt gctggacgaa gaagtctcca ccgaccaggc
     1021 gcgctggcat aaccgctact ggatcgacag cgaagggcaa attcgccagt cggaacagta
     1081 tctcggcgcg gattatttcc cggtgaaaac cactctcatc aaggcggcaa aacaatgatt
     1141 aaacaaacta ttgtcgcatt gattttgagt gtgggggcgt catcggtctt cgcggcggga
     1201 accgtcaagg tattcagcaa tggcagcagt gaggccaaaa cgctgacggg tgcagagcat
     1261 ttaatcgatc tggtaggcca accacggctg gcaaacagtt ggtggcccgg tgcggtgatt
     1321 agcgaagagc tggcaacggc ggcagcattg cgtcagcagc aggcgttgct gacacggctg
     1381 gcagaactgg ctgcagattc cagcgccgac gatgccgccg cgattaacgc cttacgccag
     1441 caaattcagg cgttgaaagt gacgggcagg caaaaaatca atcttgatcc cgatatcgtc
     1501 cgcgttgccg aacgcggtaa cccgccgttg cagggcaact acacgctgtg ggtcggacca
     1561 ccgccgtcca cggtcatgtt gttcgggctt atcagccgtc ctggcaagca gccattcact
     1621 ccaggtcgcg acgtggcgag ctatctctct gaccagagcc tgctcagcgg tgcggatcgc
     1681 agctacgcgt gggtggttta ccctgacgga cgcacgcaaa aagcgccggt ggcttactgg
     1741 aacaagcgtc acgtagagcc gatgcccggc agcattattt atgttggcct cgcggactcc
     1801 gtctggagtg agacacctga tgcccttaac gccgacattc ttcagactct gacgcagcgg
     1861 ataccccaat aatgaaaaaa agacatctgc ttagcttact ggcgctgggt attagcacag
     1921 cttgctacgg cgaaacatat cctgcgccca ttggtccgtc gcaatcggat ttcggtggcg
     1981 taggattatt acaaacgccc accgcgcgca tggcgcggga aggggagctg agcctgaact
     2041 atcgcgataa tgatcagtac cgttattact cagcttcagt gcaactcttc ccgtggctgg
     2101 aaacaacgct gcgctacacc gacgtgcgca cccggcagta cagcagcgtc gaagcgttct
     2161 ctggcgatca aacgtataaa gataaagcct tcgatctcaa actgcgtttg tgggaagaga
     2221 gttattggct gccgcaagtg gcggttggtg cgcgggatat cggcggtacg gggctgtttg
     2281 atgcggaata tcttgttgcc agcaaagcct gggggccgtt cgattttacg ctcggcctgg
     2341 gctggggata tctgggcacc agcggtaatg tgaaaaatcc gctctgttca gccagtgata
     2401 aatattgcta tcgcgataac agctacaaac aagcgggatc tatcgacggc agtcagatgt
     2461 tccacggccc ggcctcactg tttggcggcg tggaatacca gacgccctgg caaccgctgc
     2521 gcctgaaact ggagtatgaa ggtaataatt atcagcagga ttttgccggg aagctggagc
     2581 aaaaaagtaa gtttaacgtc ggggcgattt atcgcgttac cgattgggcc gacgttaacc
     2641 ttagctatga acgtggcaac acctttatgt ttggcgtcac gctgcgcacc aactttaacg
     2701 atctgcgccc gtcctacaac gataacgccc gaccgaaata ccagccgcaa ccgcaggatg
     2761 ccattttgca gcactcggtg gtggcgaatc agttaacgct gttgaaatac aatgccggac
     2821 tggccgatcc gcagatccag gcgaaaggcg atacgctgta tgtcaccggc gagcaggtga
     2881 aatatcgtga ttcgcgcgaa gggatcatcc gtgctaatcg gatcgtgatg aacgatctgc
     2941 ctgatgggat caaaacgatc cgcattacgg aaaatcgcct taacatgccg caggtgacga
     3001 cggaaaccga tgtcgccagc ctgaaaaatc atctcgccgg agagccgttg ggccacgaaa
     3061 cgaagctggt gcaaaaacgc gtcgagccag tggttccgaa gtccaccgag cagggctggt
     3121 atatcgacaa atcacgcttt gatttccata tcgatccggt gctgaaccag tcggtcggtg
     3181 gcccggaaaa cttttacatg tatcagctgg gcgtgatggg aacggcggat ttgtggctga
     3241 cggaccatct gctgaccacc ggcagcctgt ttgctaacct tgccaacaac tacgacaagt
     3301 ttaactacac caatccaccg caggactcgc acttaccgcg cgtgcgtacc catgtgcgcg
     3361 agtatgtgca gaatgatgtc tatgtgaata acctgcaagc caactacttc cagcatctgg
     3421 gcaacggctt ctacggtcag gtctacggag gttatctcga aaccatgttt ggcggcgtgg
     3481 gggcagaagt gttgtatcgc ccgctggata gcaactgggc gtttggtctg gatgccaact
     3541 acgttaaaca gcgcgactgg cgtagcgcaa aagatatgat gaaattcacc gattacagcg
     3601 tgaaaaccgg acatctgacc gcgtactgga cgccatcttt cgcccaggac gtgttagtga
     3661 aagcgagcgt cgggcagtat ctggctgggg ataaaggcgg cacgctggag atcgccaaac
     3721 gctttgatag cggcgtggta gtggggggct atgccacgat cacgaatgtt tcgaaagagg
     3781 agtacggcga aggggacttc accaaaggcg tgtatgtctc tgtaccgctg gatctcttct
     3841 cgtctggccc gacacgcagc cgtgcggcga ttggctggac gccgctgacg cgtgacggtg
     3901 gtcagcaact tgggcgtaag ttccagttgt atgatatgac cagcgaccgt agcgtcaatt
     3961 tccgctaagt catgggaaag gtgccagttt tcgcactcac tggcaccttc attcttaatg
     4021 gcctgtcgag ccatcaggaa cgcagttcac ccccggtttg cgatctggtg tgtaaaggcg
     4081 aaaacattct gtcttcctgt ccgtcgatat cggttgcagg tctggttgcc tgagcgactg
     4141 ggccatcatt gggctggaga cattgctttg cggtaggtcg gctttatcag cagtaccgag
     4201 cggaccagca taagcaggaa gaacagagac tgataacatc aaagcagcaa aataaggctt
     4261 catttttacc acctttatca ggttacgttt catttgttcc agaggaacat tgtcgatttt
     4321 tcgcgcattg ctggtggctg ggaatcacct gaatgggtga tttttttggg ctgtgtgttt
     4381 tgccagatgc gacattgatg ggagatgccc acttaattga gctgaacaaa acatacacaa
     4441 aaaatataga tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg
     4501 tactgctatg acgtcactct ctcgtccgcg cgtggagttt atatccacca ttttgcagac
     4561 cgtactcaat cttggcctgt tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga
     4621 aacggtgcat ctggctgatg tgctgttcgc gccagaacaa accagtaaat atgagctggt
     4681 agaagggctg gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt
     4741 tcagtccggt tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt
     4801 gcgcttgatc atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggcagcgat
     4861 cctgttactg gtgatcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa
     4921 aaaggcgctc cgtggagcgc cgaataacag tcacaagttg ggataacgta agttgagggt
     4981 gcagcggcat aacattggca gaacaacatc tttaaccttt cacaccacct gccgtcaggc
     5041 cgttgaccag ccagcgttga gccagcaaga agacgatggt gatcggtaat gcagacatca
     5101 cggcagcggc ggcaaagtca ccccacaggt agttttgcgg gttgaggtat tgctgcatcc
     5161 ccacagccag ggtgtagctg tttacgtcac gcagtaatag cgacgcgacc ggaacttcag
     5221 taatggcagc gataaacgac aggatgaaca caaccgccag aatcggtact gacagcggca
     5281 acaggacaag gcggaaggcc tgccacggtg tcgcaccatc cagcgccgcc gcttcttcca
     5341 gcgaactgtc gatggtttcg aaataacctt tgatggtcca gacatgcagc gcaatcccgc
     5401 ccagatacgc gaaaattacg ccgccgtgag tattcaggcc aatgaatgga atgtactcac
     5461 ccaaacgatc aaacaacgca tacaacgcga ccagtgaaag tactgccggg aacatctgga
     5521 aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa gcgcatacgg gcgaaagcgt
     5581 aggcgcaggt ggtggagagc gccacaatgc caatcgcgga aatcccggcg acctttaccg
     5641 agttccacag ccacagcagt accgggaatg gcggtggcgt aatgcgacca tcagcctgtt
     5701 ccacactaaa acctaacgcc agtttccagt gatcccagga gatttgctcc gggatcagac
     5761 taccggtcgc gaagttcccc tgacgcagcg agatggcgac gaccatcagc agcgggaaca
     5821 taatcgccgc gataaaaagt agcagtagca ggtgagtaat aaacaaacgt gctttttgcg
     5881 atttcggttg gaccattgcc atttttgtta tctcccttaa tcaaacttca ttcgcgtggc
     5941 tttcaggttc actatcgcca gcgcgcccac cagcaggaag atcagcgtgg caattgctgc
     6001 tgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg atacggtagg tgtagttaac
     6061 aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga cgatccgggc cgccgttggt
     6121 taacagttga atcagcacga agttgttaaa gttaaaggca aagctggcga tcatcagcgg
     6181 tgtcagcggt ttaatcagca gcggcagcgt aatcttaaag aagttctgga atggacctgc
     6241 gccatccatt gctgaggctt catacaaatc gtccggaatc gctttcagca agcccatgca
     6301 gaggatcatc atgtacggat aacccagcca ggtgttgacg ataatcagca tcgtgcgggc
     6361 ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac agcgcgctca acatcatgtt
     6421 gatttcaccg aaactctggt taaacaaccc tttgaaaatc aagattgaaa tgaacgatgg
     6481 caccgcgtag ggcagaataa gcaggacgcg atagaccgct ttgccgcgca acgcttccca
     6541 ctgcaccaga cacgccagaa ccatgccgac cgccaccgtt aaaaagacag tgatcagcga
     6601 gaacaccacg gtccagacga aaatggcgag gaacggtttc tgaatgcctt cgtcggtaaa
     6661 gacgcgggta aagtttttcc agccggtggt cacggtgtaa ccggggctta gcttttcatc
     6721 cccccagttg ccatcggcgg taatggactg gtaaaagcca atttggttat tcggacgata
     6781 tttcacgccg ctctgattat tcgtcaacgt gccgtcaccg tcgagtgtgt agagcggttg
     6841 cgtgccagaa aactggcgca gggagctcat catcactttg ttgccatccg gcagaatggc
     6901 ggtaatgtca ctcagtgcct gacgattctg ggtaatcacg cgcagattcg cgcgctcgcc
     6961 ttcaggctgc gtggcgcttt ctttcaattg cagtttttgc tcgccgccaa atttgaaagc
     7021 gtcggagagg taatttttgc cggtttcgcc gtcgctgagc gccagttgcc actcatcgcc
     7081 cgccggataa agaccaaaat tatagatttt gcctgcttgc caggagcgat ctagcaacac
     7141 ttcctgcgca cgttcaaaag tcagctggtt agtgctgctg tagttggtga aggcaatggc
     7201 gatggtgcag accagaggga agaggacgaa taatcccatt ccagccattc ccgggtaaac
     7261 atagcgccag gcgtaggctt tacgattggc gaaaatatac agccccgctg agctcaatat
     7321 cagcgtggta atggcgaaca ggtattcccc ttgtgcgtac attaaaacaa caaggtaacc
     7381 caccagcagg ccgagcagac ctaacactga ccatttcagc gcgtcgcttt gccaccaatg
     7441 tttcttttta atgacatcca tggggttctt cctcattcca ggacggataa ggctttcata
     7501 ccttatccga caacaactgc ctgatgcgac gctgacgcgt cttatcaggt ctacatacgt
     7561 ttcaatttcg taggccggat aaggctttca taccttatcc gacaacaact gcctgatgcg
     7621 acgctgacgc gtcttatcag gtctacatac gtttcaattt cgtaggccgg ataaggcgtt
     7681 tacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgtgc atctttcagc
     7741 gcagcatcca cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac
     7801 cagaaagcgg acatctgcgg gatgttcggc ataatttcac ctttctgagc gttatccatg
     7861 gtggcggcaa tacgtggatc tttcgcaagt tcatcctgat aggacttcag cgctactgca
     7921 cccagcggtt tgtctttatt gaccgcttcc agaccttcgt cagtcagcag atagttttcg
     7981 aggaactctt tcgccagctc tttgttcgga ctggcggcgt taatacctgc gctcagcacg
     8041 ccaacgaacg gtttagatgg ttgacctttg aaggtcggca gtaccgttac accataattc
     8101 actttactgg tgtcgatgtt ggaccatgcc cacgggccgt tgatagtcat cgctgtttcg
     8161 cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat gtgtttgttt
     8221 ttaatcagat caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc cacgcctacg
     8281 tctttaatgt cgtacttgcc gttttcatac ttgaacgcat aacccccgtc agccgcaatt
     8341 agcggccagg tgaagtatgg ttcttgcagg ttgaacatca gcgcgctctt acctttcgct
     8401 ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggcgggtt cggcagcaga
     8461 tctttgttat aaatcagcga taacgcttcg acagcaatcg ggtaagcaat cagcttgccg
     8521 ttgtaacgaa cggcatccca ggtaaacgga tacagcttgt cctggaacgc tttgtccggg
     8581 gtgatttcag ccaacaggcc agattgagcg tagccaccaa agcggtcgtg cgcccagaag
     8641 ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc cagtttatcc
     8701 ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc gacttcagcg
     8761 agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc ttcgattttg
     8821 gcgagagtcg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca
     8881 cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa
     8941 acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc tacgccccac
     9001 cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggagatgc gcacataaaa
     9061 tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg tttagttcac
     9121 agaagccgcg ttctcatcct cccgcctcct cccccataaa aaagccaggg ggtggaggat
     9181 ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt cgatgacagg
     9241 ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac gaaagcctgg
     9301 ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga attcgtggtg
     9361 tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc cgggcttgag
     9421 acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac tccgccagca
     9481 gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct gtcagtagca
     9541 gaaaacatgt catttggcct gaaactggca ggcgcaaaaa aagaggtgat taaccaacgc
     9601 gtcaaccagg tagcggaagt gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg
     9661 ctctccggtg gtcagcgtca gcgtgtggcg attggccgta cgctggtggc cgagccaagc
     9721 gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt
     9781 atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt cacccacgat
     9841 caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgctgg tcgcgtggcg
     9901 caggttggga aaccgctgga gctgtatcac tatccggcag accgttttgt cgccggattt
     9961 atcggttcgc caaagatgaa cttcctgcca gtaaaagtga ccgccaccgc aatcgatcaa
    10021 gtgcaggtgg agctgccgat gccgaatcgt cagcaagtct ggctaccggt cgaaagccgt
    10081 gatgtccagg tgggagccaa tatgtcactg ggtattcgcc cggagcatct actgccgagt
    10141 gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa
    10201 acgcaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac
    10261 gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga gcgttgccat
    10321 ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg cgtttaagca
    10381 ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag
    10441 gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg
    10501 taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc ggtattggct
    10561 ggacaggtag cggcggcgaa caacagtgtt tccagactac cggtgctcaa agtaaatacc
    10621 gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa gtgtggaaag
    10681 agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg caacagaatg
    10741 actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggtaaa aacctgatcg
    10801 aatggctgcc aggctccacc atctgggcag gtaagcgctt ctaccaacgt catgacgttc
    10861 atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg gaaaacatcg
    10921 atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagcg ggtggttctt
    10981 cctctttcgc cagcaacaat atttatgact ataccaacga aaccgcgaac gacgttttcg
    11041 atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg ggtgtcgact
    11101 acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg aaagacggct
    11161 ggttattcac tgctgaacat actcagagtg tcctgaaggg ttttaacaag tttgttgttc
    11221 agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg
    11281 cgtttgataa cgaaaaattt gcctacaata tcaataacaa cggtcacatg ctgcgtatcc
    11341 tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc
    11401 aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgtcggt attcgcccga
    11461 tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat
    11521 cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg
    11581 ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg
    11641 atgagaaatg gggttatgac tacaccggta gctcttcaac caacccatac tacggcaagg
    11701 ctgtgtcggc tgatttcaac ggcggtagct tcggtcgtgg cgacagcgac gagtggacct
    11761 tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggcctgata aggtgcttgc
    11821 gccgcatccg gcaaccaatg cctgatgcga cgctgttgcg tcttatcagg cctacaaccg
    11881 ctgccgaaag taggccggat aaggcgttta cgccgcatcc ggcaaccaat gccggatgcg
    11941 acgctgttgc gtcttatcag gcctacaact gctgccgaaa gtaggccgga taaggcgttt
    12001 acgccgcatc cggcaaccaa tgcctgatgc gacgctgttg cgtcttatca ggcctacaac
    12061 tgctgccgaa agtaggccgg ataaggcgtt tacgccgcat ccggcataaa aacagcttta
    12121 aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct accgctgagc
    12181 tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgctc tctgtttatc
    12241 agcagggttg ctggcaagcg cacctggaat cagccttgcc gatgttaact acgtaccgca
    12301 aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca cctggacacc
    12361 ggtcgatcaa tctaaaaccc agactactca actgtcgacc ggcggccaac aactgaatgt
    12421 ccctggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg gtgaactgac
    12481 actgacgctg accagcgaag tgaacaaaca aaccagcgta tttgcgccga atgtgctgat
    12541 tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca cctaccagga
    12601 acccggcgtg atgagtgcag atcggctgga aggcgttatg cgcctgacac cggcgttggg
    12661 gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc agacgactca
    12721 actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc cggatattcc
    12781 cgatccggtt gctcgtcata ccaccgatgg cttactgaaa ctgaaagtga aaacgaactc
    12841 cagctccagc gtgttggtag gaccactatt tggttcttcc gctccagctc cggttacggt
    12901 aggcaacacg gcggcaccag ctgtggctgc acccgctccg gcaccggtga agaaaagcga
    12961 gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg ctgtcgcgaa
    13021 aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg ggtcgacatc
    13081 tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc ccacagtgct
    13141 gattttgcaa caactggtgc gtctcctggc gcaccttttt ttctcctcct gtactattgt
    13201 tgcgtttttg ttgcgcatta gttcacttaa tttgctttac atctcccgta aatatttttc
    13261 tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa
    13321 cgcaactgcg tgcgctgcgc tattttacag agatccctgc cctggagccg caactgctcg
    13381 actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga aaaacggtga
    13441 gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa gaactgccac
    13501 tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac
    13561 cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt gagcgggccg gagctggcgt
    13621 tacaaaaatt aggtaaaacg ccgttaggac gctatctgtt cacatcatcg acattaaccc
    13681 gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat
    13741 taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag
    13801 aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa
    13861 tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt
    13921 gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc gcgggcgtct
    13981 ggctgatgcg cgctgctggt tgcgtggtga atgattatgc tgaccgcaag tttgatggtc
    14041 atgttaagcg cacggcgaac cgaccacttc ccagcggcgc ggtaacggag aaagaggcgc
    14101 gcgcgctgtt tgtagtgctg gtactgattt catttttact ggtgctgacg ctgaatacga
    14161 tgactattct gttgtcgatt gccgcgctgg cgctggcgtg ggtgtacccg tttatgaagc
    14221 ggtataccca tctaccacaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg
    14281 cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc ctcgccaata
    14341 ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat gatgatgtga
    14401 agattggcat taaatccacc gcaatcctgt tcggccaata cgataaattg attattggta
    14461 ttttgcagat tggcgttcta gcgctgatgg cgatcatcgg tgagttaaat ggcttaggct
    14521 ggggatatta ctggtcaatt ctggtagctg gcgcgctgtt tgtttatcaa caaaaactga
    14581 ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc
    14641 tggtactgtt tttagggctg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg
    14701 gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag gcctacacaa
    14761 catatcgcaa tttattgaat ttgcagatca tggtaggccg aataaggcgt ttgcgccgca
    14821 tccggcaatt ctcactgatt acccttcgcc ctgtgtcgca ctctcaatcg tcaaacgcac
    14881 gtctgatgta atcaactccg ccagcaactg atataccttc atcgtttctg ccggttcggc
    14941 gtcgccgcta tcgctgatat acccttcatc acgcagtgtc agcaccagag aactgaacac
    15001 cgccttgtcg aagaactccg gcgcgttgat accgtgcagc acggagagac gttgcgcgac
    15061 ggtgcggctc tctttctcca gtgtaccgcg gttgatcgac gggttggcac tcaacagcca
    15121 gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagtgt
    15181 gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg
    15241 ttgcatctca tttgccagcg catcaataac gtccggtaac tcgtcgcgat cccagcgcag
    15301 gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg
    15361 ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg
    15421 tgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca gaatgatgat
    15481 gtcgccgata gtgtctttct caacttcaaa cttgttcatt tgcagcgcgt gatcgataag
    15541 ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggcacgt tgcgcatcag
    15601 atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga
    15661 cgccagaagc gcagtacagc acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg
    15721 taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg gacgcaccgc
    15781 ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa
    15841 cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc
    15901 acgcagcatc tgcggcaggc tctctttctc tttcgtcgca ccgcgcagtt ctttggcgta
    15961 agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg taatcggacg
    16021 agtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag
    16081 caaacgcccc gtacgggaac gaccgccttc cacgaaatac tcgacggaat aaccacggct
    16141 gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc ctttaaacgt
    16201 acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaagtttag
    16261 gttgatcccg gcggcgatat gcggcggcac cagcccttgg tgatacagca cgtaagaaag
    16321 cagcaggtag tccatgtgac tgcggtggca aggcacatac actagctcat ggccgtcgtg
    16381 ggccagctgg cgaacgcgct cggcgttatg gacgttgata ccctggtaaa gtcggttcca
    16441 ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat tcgccgcaat
    16501 ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg
    16561 cgcttcatct tctaccgctt tcgcaatggc gcgggaggcg agcagcttat taaacagatc
    16621 ctgacgagca ggcagacgtg gacccacggc agccaggcgt tgacgggcaa agtgcatacg
    16681 cgccacgcgc gccagtttct gagcgatagt tttatccgtg ccgtgttcat ccgccatacg
    16741 gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacagc
    16801 gaaaaatttc tgtacaccgt taagcatacg cagcggcggg ttcacttcgc ctttttcacg
    16861 ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt
    16921 gctgcggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt tcggcgtgta
    16981 ataggtgaac acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccatc
    17041 gatttccagc ggctctaacg ggtcaggcag gtcatgtgcc agacactggg cgcgcaacgt
    17101 cagcaagtca gctttcgagt tgtacggtaa aacgtacata attggacgag aggtatccag
    17161 ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg
    17221 taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct
    17281 ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc
    17341 cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat
    17401 attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc
    17461 gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag
    17521 cggcattccg tcaggaaggc gtagcggtat tgttagcggt ggttatcgcc tgctggctgg
    17581 atgtggacgc gattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg
    17641 aaattctcaa tagcgccatc gaagctgtgg ttgaccgaat tggctctgaa taccatgagc
    17701 tttccggacg cgctaaagat atgggatccg ccgcagtgct gattgccatt atcgtcgccg
    17761 tgattacctg gtgcatcctg ttatggtcgc attttggata acccttccag atttcgataa
    17821 atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta tatactcaca
    17881 gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag gcaacaagag
    17941 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
    18001 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
    18061 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tttattgcag
    18121 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg
    18181 caacagcata tcgaaggtca ttatcaggtc gatccttcct tgttcaagcc gaatgctgat
    18241 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggcgatttg
    18301 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
    18361 gacgaagtta ccgttaaacg cctgaaaaaa cagggcaata aagtcgaact gttaccagaa
    18421 aacagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac cattgaaggg
    18481 ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga gaccgcgatg
    18541 ccgcctggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg catggcattc
    18601 ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt ctccaatatc
    18661 accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga tagtccggtt
    18721 tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat gttgttgctg
    18781 tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa aaatcctcag
    18841 gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggccgg ggcgttaatt
    18901 gcgctgctgc gtacgccgat tatcgatctg gcgctgcata ttgttggtgg tagtgaagca
    18961 gtcctggaac aggcgcgacg ctttcttgaa atccgctggt taagcgcacc ggcgtcgctg
    19021 gcgaatctgg tattacttgg ctggttactc ggcgtgcaat atgcccgtgc gccagtaatt
    19081 ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt gatggggctg
    19141 catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc aacattgctg
    19201 attggtctgc taatggtgcg taaaattctc aaactacgcg gaatctccgg cgaaatgctg
    19261 aaaacagcct ggcgaggaaa cttccgtcac ttgctggcgc ttaaccgcga tatcatgctg
    19321 cgctcgctgt tgctgcaact ctgtttcggc gcgatcactg tacttggcgc gcgactgggg
    19381 agtgacatta tcgctgttaa cgcagttctg atgacgctac tcacctttac cgcctatgcg
    19441 ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg tgcgcgcgac
    19501 ggtagtcaat tgctggatgt ctggcgggca gcgtgccgcc agtcggggat cgtagcgtta
    19561 ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct gacgtcgtta
    19621 acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt gccgttggtc
    19681 ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc cgccgaaatg
    19741 cgtaacagta tggcggtggc cgccgcaggt tttgcactga cgctccttac gctgccgtgg
    19801 ctggggaatc atggtttgtg gctggcgtta accgtctttc tggcgttacg cgggctttct
    19861 ctggcggcta tctggcggcg tcactggcgt aacgatacct ggtttgtcgc aacgtgacgg
    19921 ttaaaaattc tgaataaata atcctaagcc aaattgctga ctacacttaa tctcacgttc
    19981 agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga ttatgaataa
    20041 agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa
    20101 actgaccgat gatgatatga cgatcattga aggaaaacgt gatcagttag tgggtaaaat
    20161 ccaggaacgc tacggttatc agaaagatca ggcagaaaaa gaggttgata gttgggaaaa
    20221 acgccatgat taccgctggt aattaatccc tcctgcccga catgtacaag gatgtacacc
    20281 cttttagcga ggtttctttt tcacctgaat agagtggtca tgatggcact gttcgggatg
    20341 acgacacgct tctacttcta cacatgctga acataatcca tgtgcttcaa taacattatg
    20401 ccgcagggca aaacccattt ttgccgccag cgtatgcatg atgtcttcca cgccttcagc
    20461 acattcttct ttcaccgcgc cgcagcgatc gcaaataaac atggctgaag tatgggtggg
    20521 ctgatcgaac agatgacaga gcacataact gttggtggat tccaccttat gcacaaaacc
    20581 ttgttcaagc agaaactcca gcgcgcgata aaccgttggc ggcttggctt gcggttcagc
    20641 ttcgcgcagc aaatcaagca gatcataagc gctgatagcg ccatcctgca gactcatcag
    20701 gcgcaacact tcgagacgct gtggggtcag gcgcacatta cgctgtgcgc agattttttc
    20761 agcctgcgct aataactcct gcgttgtggt cttttccatt gggcacctcg acagtggggt
    20821 taagaatgcc cttactttac catagttctc gcttaaacgc cgagaacagg gtgtcatggc
    20881 tgtcacattg cctgatcgca tattcgttat gctatagtag cgcccccaat ttaccagatg
    20941 cttaaaaagt cgccatgcca gcagaatcac acactgttta tcccgcttac cgtttctcga
    21001 ttgccccgat gctcgactgg acggacagac attgccgtta tttcttgcgt ctgctttccc
    21061 gcaatacgtt gctgtatacc gaaatggtga ccacaggggc gattattcac ggtaaaggtg
    21121 attacctggc gtacagtgaa gaagaacatc cggtagcgtt gcaactcggg ggtagcgatc
    21181 cggcggcgct ggcacagtgt gcgaagctgg cagaagcgcg tggatacgat gagatcaacc
    21241 tgaatgttgg ctgcccgtct gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg
    21301 gtaatgcgca gctggttgcc gactgcgtga aagcgatgcg cgatgtggtg tcgattccgg
    21361 tgacggtgaa aacgcgtatt ggcatcgatg atcaggacag ctatgaattt ctctgcgatt
    21421 tcatcaacac cgtttccggc aaaggtgagt gtgagatgtt catcatccac gcacgtaaag
    21481 cctggctttc tgggttaagt ccgaaagaaa accgtgagat cccgcctctc gattatccgc
    21541 gtgtgtatca actgaagcgt gactttccgc atctgacgat gtcaattaac ggtggtatca
    21601 agtcgctgga agaagctaaa gcgcatttgc aacatatgga tggcgtgatg gtcgggcgcg
    21661 aggcgtatca gaatccgggg attctggcgg cggtagaccg agagattttt ggttcctcgg
    21721 ataccgatgc cgatccggtg gcggtagtgc gcgccatgta tccgtacatt gagcgtgaac
    21781 tcagccaggg tacgtatctc ggccatatta cccggcatat gctgggcttg ttccagggta
    21841 ttcctggcgc gcggcagtgg cggcgttatt taagtgaaaa tgcccataaa gcgggtgcag
    21901 acattaatgt gctggaacac gcgctcaaac tggtggcgga taagcgttaa cttttcacca
    21961 aaaagtagtc aaattcacca cgccctgcgc accgtcgcgg ggtgttttgc tgttaaatca
    22021 acagattatt tttggcatga ttcttgtaat gccagcaaga gatttcatat ttgggagagc
    22081 atcatgctgg aactactttt tgtaattggc ttttttgtca tgctgatggt caccggtgtt
    22141 tcgttgctgg gtattatcgc cgcgctgatt gtggcgacgg cctttatgtt cctcggcggt
    22201 atgctggcat tgatgattaa gttgctgccg tggctgctgt tggcggttgc ggtggtgtgg
    22261 gtgatcaggg cgattaaagc accaaaagtg ccgaaatatc agcgttatga ccgctggcgt
    22321 tactaaggga ttgtgcggat gatcacaacc taaggtttta tccttagaac aaaataggaa
    22381 ttgataatca aatctgtcac tattgcgcgt caaacaaatt catcgagctg taccctacat
    22441 acagccgaat tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat
    22501 caataggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgtgcatc
    22561 cttaagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa
    22621 agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag
    22681 ggaagggcgt gttacataca acgagccttt ctgattgaga atgcctaagt tcacaccggt
    22741 aaccgcacct gatgagttgc caaaactcac cattaagccg cggcgttgca ggcaatccag
    22801 cgaccgttcc caggtgtctc tgcccactga atcgtacacc acacgcactt tcttaccacc
    22861 ggtgatctct ttcaatcgct cgaccagatt ctcttcacga tagttaataa cctgccacgc
    22921 gcccgctttt agcgcactct gcgctttttg cgcggttcct acggtgccga taagtttcgc
    22981 gcccagggct tttgcccact ggcaggcaat caagccaacg ccgccagccg ctgcgtggaa
    23041 caggaactgc tcatcgggtt taatttcata ggttttgcgc agcagataat aaaccgttaa
    23101 gcctttcagg aaggatgccg cagcttgctc aaaagaaatt gccgcaggca gaatcgccgc
    23161 tttatctgca ttaatgttat gcacagagct gtaagcgcct aatgccgatt gtgcatagac
    23221 tacgcgatcg cctgccttaa tatgctttac accactgccg actttactca cgatgcctgc
    23281 cgcttcggtg cctaatccgc tgggtagcga tggtggcggg taaaggccgc tgcggatata
    23341 cgtgtcgata aaattgatgc cgatggcttt attttcgacc tggatttcat tctccgccgg
    23401 atcggcagga gtgaactcta cggcttgaag tacttccggg ccaccgtgct tgtgaaattc
    23461 aattcgtatt gccatgtgtc ctccagaacg tatcgtcagg gtctgcttca tatggtaaag
    23521 tttcgaccca ttctttatct cggtaactcc attcactatg gcaggaaata aacccttcaa
    23581 caaacagcag gctgaacccc gcgaacgcga tccacaagtt gccgggctga aagtgcctcc
    23641 gcactcgatc gaagcggagc agtcggtgtt gggcggttta atgctggata acgaacgctg
    23701 ggacgatgta gccgagcgtg tggtggcaga cgacttttac acccgcccac accgtcatat
    23761 ctttactgaa atggcgcgtt tgcaggaaag cggtagtcct atcgatctga ttacccttgc
    23821 ggaatcgctg gaacgccagg ggcaacttga tagcgtcggt ggtttcgctt atctggcgga
    23881 gctgtcaaaa aatacgccaa gtgcggcgaa catcagtgct tatgctgaca tcgtgcgtga
    23941 acgtgccgtt gttcgcgaga tgatttcggt tgcgaatgag attgccgaag ccggttttga
    24001 tccgcagggg cgtaccagcg aagatctgct ggacctggct gaatcccgcg tctttaaaat
    24061 tgccgaaagt cgtgcaaaca aagacgaagg gccgaagaac atcgccgatg tgctcgacgc
    24121 cacggtggcg cgtattgagc agttgtttca gcagccacac gatggcgtta ccggggtaaa
    24181 caccggttat gacgatctca acaaaaaaac cgctggcttg cagccgtcgg atttgatcat
    24241 cgtcgccgcg cgtccgtcga tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc
    24301 ggcgatgttg caggataaac cagtacttat cttctcgctg gagatgccat cagaacagat
    24361 tatgatgcgt tctttggcgt cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca
    24421 gctcgatgat gaagactggg cacgtatttc cggcaccatg ggtattttgc tcgaaaaacg
    24481 taatatctat atcgatgatt cctccggcct gacgccaacg gaagtccgtt cccgcgcgcg
    24541 ccgtattgcc cgtgaacacg gcggcatcgg gcttatcatg atcgactacc tgcaactgat
    24601 gcgcgtaccg gcgctttccg ataaccgtac gctggaaatt gcagaaatct cccgctcgct
    24661 gaaagcactg gcgaaagaac tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc
    24721 tctggaacaa cgtgccgaca aacgcccggt caactccgac ctgcgtgaat ctggctctat
    24781 cgagcaggat gcggacttga tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag
    24841 tgatttaaaa ggcatcgcgg aaattattat cggtaaacaa cgtaacggcc caatcgggac
    24901 ggtacgcctg acctttaacg gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta
    24961 cgacgacgaa taataattat tttatgaatt aggtaattaa agcaaacact tatcaaggaa
    25021 cacaaatgca agcggcaact gttgtgatta accgccgcgc tctgcgacac aacctgcaac
    25081 gtcttcgtga actggcgcct gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg
    25141 gtcacggtct tcttgagacc gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc
    25201 gtctcgaaga agctctgcga ctgcgtgcgg ggggaatcac caaacctgta cttttactcg
    25261 aaggcttttt tgatgccaga gatctaccga cgatttctgc gcaacatttt cataccgccg
    25321 tgcataacga agaacagctg gctgcgctgg aagaggctag cctggacgag ccggttaccg
    25381 tctggatgaa actcgatacc ggtatgcacc gtctgggcgt aaggccggaa caggctgagg
    25441 cgttttatca tcgtctgacc cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc
    25501 attttgcgcg cgcggatgaa ccaaaatgcg gcgcaaccga gaagcaactc gctatcttta
    25561 ataccttttg cgaaggcaaa cctggtcaac gttccattgc cgcgtcgggt ggcattttac
    25621 tctggccaca gtcgcatttt gactgggtgc gcccgggcat cattctttac ggcgtatctc
    25681 cgctggaaga tcgctccacc ggtgccgatt ttggctgtca gccagtgatg tcgctaacct
    25741 ccagcctgat tgccgtgcgt gagcataaag ccggagagcc tgtcggttat ggtggaacct
    25801 gggtaagcga acgtgatact cgtcttggcg tagtcgcgat gggctatggc gatggttatc
    25861 cgcgcgccgc gccgtccggt acgccagtgc tggtgaacgg tcgcgaagtg ccgattgtcg
    25921 ggcgcgtggc gatggatatg atctgcgtag acttaggtcc acaggcgcag gataaagccg
    25981 gggacccggt cattttatgg ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga
    26041 aagtaagtgc ttacgaactt atcacgcgcc tgacctcaag agtcgcgatg aaatacgtgg
    26101 attaatcgtt ctttaatatt tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg
    26161 ccaataaaat cgtaccaatt caataagttg atattctttc atgctcttat aaaggtcgtg
    26221 catctggcgg atgtacgttt gtcatgagtc tcactctgtt gctaattgcc attcgcccct
    26281 gaacatcctc tcgatcttcg ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa
    26341 acctggagaa ccatcgcgtg tttcaaaaag ttgacgccta cgctggcgac ccgattctta
    26401 cgcttatgga gcgttttaaa gaagaccctc gcagcgacaa agtgaattta agtatcggtc
    26461 tgtactacaa cgaagacgga attattccac aactgaaagc cgtagcggat gcggaagcgc
    26521 gcctgaatgc gcagcctcat ggtgcttcgc tttatttacc gatggaaggg cttaacagct
    26581 atcgccatgc cattgcgccg ctgctgtttg gtgcggacca tccggtactg caacaacagc
    26641 gcgtagcaac cattcaaacc cttggcggct caggggcatt gaaagtgggc gcagatttcc
    26701 tgaaacgcta cttcccggaa tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg
    26761 tagcaatatt cgccggggct ggattcgaag taagtactta cccctggtat gacgaagcga
    26821 ctaacggcgt gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta
    26881 ttgtgttgct gcatccatgt tgccacaacc caacgggtgc cgatctcact aatgaccagt
    26941 gggattccgt gattgaaatt ctcaaagccc gcgagcttat tccattcctc gatattgcct
    27001 atcaaggatt tggtgccggt atggaagagg atgcctacgc cattcgcgcc attgccagcg
    27061 ctggattacc cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc
    27121 gtgtcggcgg actttctgtt ctgtgtgaag atgccgaagc cgcaggccgc gtactggggc
    27181 aattgaaagc aaccgttcgc cgcaactact ccagcccgcc gaattttggt gcgcaggtgg
    27241 tggctgcggt gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga
    27301 tgcgaactcg cattctggca atgcgtcagg aactggtgaa ggtattgagc acagagatgc
    27361 cagagcgcaa tttcgattat ctgcttaatc agcgcggcat gttcagttat accggtttaa
    27421 gttccgctca ggttgaccga ctacgtgaag agtttggggt ctatctcatc gccagcggtc
    27481 gcatgtgtgt cgccgggctt aacgcgcaaa atgtgcatcg cgtggcaaag gcgtttgctg
    27541 cggtgatgtg attttaatca acaggcggat ctctccgcct gttcctgact ccagccatcc
    27601 cctactattc tgtccctttt ttcgcccact tttacatcat ctattttcta taagtttttg
    27661 aaaattagtg tttttaactg ttggtacgga ttgtgcagta atgcggctgt ctatgaccat
    27721 caggagaaaa acatggagaa catgacctcg cccggcacgc tgcttagtgg agataacgcg
    27781 acctggcttg aagattacta ccagacctgg ttgcgtacgc cggaacaact cccggaagac
    27841 tggcggcgtt tttttctttc acctgaactg actgtccaaa gcgtatccgg tgataacaac
    27901 gtcagcggtg cgaccttaaa aaaacaggct gcgataatac aactgatcaa tgcctggcgc
    27961 acgcaggggc atctgcgcgc aaagcttgat ccacttggtt tgaatccccc agctgacgtt
    28021 ccctccctcc tgcctggttt ttggggatta agcgaagaag acttgttgca ggagtttagc
    28081 gtcacctttg gcgcacacac cacgcaaatg ccacttaagc aactgcttaa cctgctagag
    28141 caagcctggg cgggtagcca ggcttatgaa cttgctcatc tggaaaatcg tgaagaaatc
    28201 aactggttgt tatcacggat tgagagcagc aatgcgccac aagcagatgt acaaacctgt
    28261 attgctcgtt ttgaaaaact gatggcggca gagacgttag agcgttatct gcatacccgt
    28321 tatgttgggc agaaacggtt ttctctggaa gggggcgaaa gtgcaatccc ggcgctcgat
    28381 acgctgacaa aatgcctgcg tgcgcaaggt gtggaggaaa tggtcattgg catggcgcac
    28441 cggggaagac ttaatgttct ggttaatctg ttgaataaag acccggcaca gctttttgct
    28501 gagtttgaag gcaagcagac gatcggcagt ggttcaggcg atgtgaaata ccacatgggg
    28561 tattccagca atctggaaac acctgccggt tcgctgcatg tggcgctggc gtataacccc
    28621 tcacatctgg agatcgtgaa tccggtggtg ctgggccagg tgcgagccag gcaggaacgc
    28681 cgtggtgagg atggtcaggc aaaagtggtg ggcgtactta ttcatgggga ttccgcgttg
    28741 ggcggcctcg gcgtcaacca aaccacgttt aacttatcgc agactcaggg ctacggcact
    28801 ggcggtacgc tacatctggt gattaacaat cagattggct tcaccacttc ccgcttgcag
    28861 gatatgcgtt cttcacggta ctgcaccgat attgccaaaa tggtagcggc cccaattatc
    28921 cacgttaatg gcgatgacgt tgacgctgtt tgccaggtaa tggaactggc gtgcgaatgg
    28981 cgagacacct tccggcgcga catcatcatc gatatttgct gcttccgtaa gcacggacac
    29041 aacgaaagcg atgagccacg tcttacccag ccacaaatgt atcaggcagt ggatgctcac
    29101 cctggtacgc tcgcccgtta tggtgaatct cttgcccgcc gcggcctgtt aactcaggcg
    29161 caacaagatg agatgacggc acgctatcgc gactggctgg atagctgcca gaaacgtgaa
    29221 ccgcagcctc tcaaacctgc aattcactct tttagcgcta actggtatgg gttgaccaac
    29281 cctcactgga gcgccccggt atctacggca ttgcctcggc aaaagctggc agcgtatggt
    29341 gaaatcattt ctacactgcc acccgatgtg gtcgcacatc ctaccattaa acggcaactg
    29401 acgttacgcc aggatatggc cgcaggtact cagccaatag actggggaat ggctgaaatg
    29461 ctggcctatg catcgcttgt ggatgcgggt gttggtgtgc gtctttctgg tgaagactca
    29521 gggcgtggca cgttcagtca tcgccatgca gtggtacatc atcaaactga agcccgtcgt
    29581 tatctgccgt tacagcatat ccgcgctggt caggcatctt tcgatgttta tgattcggtg
    29641 cttaatgaag aagcgctctt agcctttgaa tatggctatt caacgtctgc gcctcagcaa
    29701 ctggttattt gggaagcaca gtttggtgac tttgccaacg gcgctcaggt tgctattgac
    29761 cagtttatct cgtctggcga aaccaagtgg gatcgctata gcggtttaac cattcttctg
    29821 ccgcatggct atgacggaca agggccggag cactcttctg cgcgtcctga gcgctggctg
    29881 caactgtgcg cagaaaacaa catgcaggtg gtgatgccca gtgagtcagc acaaatgttc
    29941 catttgttgc gcggtcaggc attacgccca atgcgcaaac cgttagtgat aatgatgagt
    30001 aagcgtttac ttcggtttaa aggcgctatg agcgagcttt ctgaattcac tgatggcgct
    30061 tataaaccgg tagtcaccga tccgcaattg catcagtccc agaaagttaa gcgtgttatt
    30121 ttgtgtagcg gccaggtcta ttacgacgta ctggaagccc gtaagcagcg tgagtgtgaa
    30181 gatgaggtag cgattgttcg cctggagcaa ctctacccgt tcccggtcgc agaactgaat
    30241 gatgttctgg cgagttggcc gaactgctgc gaatggattt ggttgcagga agagccagaa
    30301 aaccagggcg cgtggcggca gattcgtcat gagctggcgg cactgaaaat taacactcct
    30361 tactggcagt atgcaggccg cccggcagcg gcagctccgg cgacagggta tgggcgtgta
    30421 cataaacaac aaatcgacga atttcttgct gctgcgtttg cagatattca gccatgaaag
    30481 gcgcacaaaa ataataagga gaacatttta tgatcgaaat tactgtccct gtattaccgg
    30541 aatctgtcac ggaaggcacg ctgacaacct ggtgtaaaca agaaggcgag cacgtaaaac
    30601 gcgatgatgt gattgccgag ctggaaaccg ataaagtcat tctggaaata ccggccccgc
    30661 acgatggcgt gttaagcaat attatcgtca gcgaaggtag cacggtcacg tccgcgcaac
    30721 tgttggcgca tcttaagccg caagctgtca tagaagaaac cgtcacacca gtcaccgaaa
    30781 ccctggcgat gccttccgca cggctggagg cacagcgtag tggtgtcgaa cttgctgacg
    30841 ttgcggggag cgggagaaat ggacgtatcc tgaaagagga tgtacagcgc gttactcctg
    30901 caccagtcat tcagcctgaa cgggtggcgg agatcgcgcc agctaaaccc ttgacaccgg
    30961 gtgctcgtca ggaacgtcgt gagccgatgt cgcgcttacg tcagcggatt gctgagcgtc
    31021 tgctggcttc ccaacaaaat aacgccattc ttaccacatt caacgaagta aacatgcaga
    31081 gcgtgatgga cttacgcgcc cgctggaaag atcgttttgc cgagaagcac ggcgtgaagc
    31141 tgggctttat gtccttcttt gtgaaggccg ttacccgggc gctggagcgc ttccctgtag
    31201 tgaatgccag cgttgatggc aacgagatta tctggcgtga ttattgcgat atcggcattg
    31261 cagtgagcag caaccgcggc ctggtagtgc ctgtgttacg caatgcgcaa tcactctctt
    31321 tggtggaaat tgaacggcaa attgccgaat acgccacaca ggcacgcaat ggcaaattgc
    31381 cgctggaggc gttacagggc ggcacgttct ctatcaccaa cggcggtact tttggttcga
    31441 tgatgtcaac gccaatcatt aacccgccgc agtcggccat tcttgggatg catgccatca
    31501 cgccgcgccc ggtcgcagaa aacggccagg ttgttattcg tcccatgatg taccttgctc
    31561 tcagctatga ccatcgcatt atcgatggtc aggaagccgt gcagacattg gtggcaatac
    31621 gtgagctact ggaatcaccg gaacaactgt tgctggatct ttaaggaggg cgccgatgag
    31681 tacaattttt gatgtggcag ttatgggggg tgggccaggt ggttacgtag cggcactgcg
    31741 ggctgcgcag aatgggcttt ccgtagtatg tattgatgat ggtgttaatg cacagggcga
    31801 accttcgccg ggcggtacgt gtctcaatgt cggctgtatt ccgtcaaaat cgttgctgca
    31861 atcttccgaa ctgtacgcgc aagtgcagca tgaggcgagc attcatggcg ttaatgttga
    31921 cggcgtatct ttcaatgcag ctgcgatgat tcaacgtaaa gacgccatag taagccgcct
    31981 gacaatgggc atcggcttgc tgtttaagaa aaataaagtg aagtacctgt gcggcctcgc
    32041 aacgttagaa catgctcggg atgagatttg gcaattacgt gtaaacgatc aacaaatccg
    32101 cgcccgtaat gtggtgattg caacaggttc tcagccgcgt caactgcctg gcgttaccat
    32161 tgataatcag caaattctcg ataatcgcgg cgccctagcg ttaaccgctg tgccgccgcg
    32221 tctcggtgtg atcggcgctg gcgtgattgg ccttgaactg gggtctgtgt ggaatcgggt
    32281 cggttcagac gtcacgttgc tggagatggc gccaacgttc ttgcctgcac tggaagctcg
    32341 cctgtcgaac gaagtccgta aagcaatgat tgccagtggg atgaaaatgc aactggcagt
    32401 agagattgag gctattgaac aacgggatga cggtgtgcat gtgcgctggc ggcagggtga
    32461 gaagcgagaa gaaagccgct tcgataaatt aattcttgcc attggacgtg taccgcgact
    32521 ttctggtgtc gatctcgtgc aactcggtct tgaagccgac aaccgtggcg gtattgccgt
    32581 cgataactta tgccgtaccg ggaaagccgg gctgtgggca attggtgatg tcgtgcgagg
    32641 gccgatgctg gcgcataaag cgatggctga aggtgtggtg gttgcggatc aaattgccgg
    32701 actggcggta gagccgatta attttgcgct gatcccttcg gtgatttaca cccaaccgga
    32761 agttgcgtgg gtaggggaaa acgaggcctc actgaaagcc gctggcagag tctttaacaa
    32821 aggaaattct ctgtttgccg gtaacggtcg cgccttagca ttaggccagg aaggtgggcg
    32881 ttgcacgtta tacagcgata aacacactga ccgcgtactt ggcggagcga ttgttggccc
    32941 gcaggcatcg gagctgatca atgaaattgc gctggcgatg acttttagcg cctctggcga
    33001 ggatattgcc tgtgcgatcc atgcgcatcc gaccttaagc gaagtcatcc acgaagcagc
    33061 aatggccttg aataataaag cattacacgg ataacacata ccactgaggg aatatatgaa
    33121 tttacatgag tatcaggcca aatcgttact ggctggcatg gggatgccgt gtccaaaaga
    33181 gatagccatc caacagattt cgcaactggc agatgcctgg caacatattg cttgcccgag
    33241 taaaggcgcg gtacttaaag cgcaggtaca tgctggtggg cgcggtaaag ctggcggcgt
    33301 aaaagtgctt aagcagctgc cggaagcaca ggcttttgta cagcaaatgt tgggatcgca
    33361 actggtgact tatcagaccg ggccagaagg gcagtatgtc agcagtattt tgctgtgcga
    33421 aaacatctat ccggtacgcc aggaactcta ttttggcatg gtggtggatc gtgaaagcca
    33481 gcgggtcacg tttattgtca gcccggaagg tggtgtggaa attgaaaaag tcgcccatga
    33541 gacaccggag aaaatcagca gtgtcagcat tgatccactg acaggcgtgc agccttgcca
    33601 tatccgcgaa atgtttgctg ttctgcaact ggaacacggg ctatttgcta cctttagtcg
    33661 cctggttaat caggcgtgga aagcctttaa cgaactggat ttcgccctgc tggaaattaa
    33721 ccctctggtg ttgcgggaaa cgggcgaatt tatgtgtgca gacgcgaaag tatcgctgga
    33781 tgataacgcg ctgtatcgtc acccggaact ccaggtgcta cgtgatgaaa cccaggaaga
    33841 tccacgcgaa agccaggcag cgaagcttga tcttaattat gtctcgctgg acggaaatat
    33901 cggctgcatg gtcaatggtg cagggctggc tatggcaacc atggacatca tcaagctata
    33961 cggtgaacaa cccgcaaact tcctggatgt tggcggtggc gcaacccagg agcgcgtcag
    34021 tgaagccttt cggctgattg tttccgacag caaagtgaaa gccattctgg ttaatatttt
    34081 tggtgggatt gtccgttgcg acatgatcgc cagagccatt attcatgctc tgaatgaggc
    34141 ccgtatcacc ctgcctgtcg tggtgcgtct ctccggtaat aacgctgccg aagggcagcg
    34201 gttactggca gaaagtggcc tgacggtgga agccgtcaat tctctggatg atgccgctaa
    34261 acgcatcatt gcgttactga attaacagga ggttaagatg agcgttttga tcaacaaaca
    34321 cacgcgggta ctggtgcagg gcattaccgg caaaaacggc acatttcata cagagcaggc
    34381 tattgcgtat ggcacgcaga ttgtgggtgg agtgacgcca ggtaagggcg ggcaacggca
    34441 tctggatcgc ccggtttttg attcaatgaa agaagcgatg cgacaaactg aggctgatgc
    34501 cagcgtgatt tatgtgcctg caccgtttgt gctggattct gttgttgagg ctattgaagc
    34561 tggcgtcaaa ttggttgtgg tgatcactga aggtgtaccg acactggata tggtgaaagt
    34621 tcgtcggctg cttgactgcc acccggatgt gcggctgatt gggccaaact gcccaggagt
    34681 tatcacgccg ggggagtgca aaatcgggat tatgcctggt gaaatccacc gcccagggcg
    34741 tattgggatt gtttcccgct ccggtacatt aacctacgaa gctgtagcgc aaaccacggc
    34801 gctgggactt gggcaatcca cctgtattgg tattggcggc gatccggttc cggggactaa
    34861 ctttattgat gcgctgcgtt tattcgaaaa tgatccgcaa acagacgcgg taattatgat
    34921 cggtgaaatt ggtggtaatg cggaagaacg ggccgcggaa tttattcgcc atgaaatgaa
    34981 taaaccggtc gtaggttata ttgccggtgt tactgcacca aaaggaaaac gcatggggca
    35041 cgccggagcc attatttctg gcggtagcgg cagtgcagag gataaattcc gtgcgttcga
    35101 taaagcaggc atggcatgga caagaaaccc ggcgttgctt ggcgaaacgt tatggaatgt
    35161 tattaagtaa tccataaatg aatatcaagg gagcatatcg ctcccttttt ttatctgtgt
    35221 ggtaagagaa tcacctttta tttactgtgc ggaaaaccgc gcacaaaata aaaagaataa
    35281 ataataaaat cattaaataa caaagcatta ttaaaaaatt aaagatggca tagctcctgc
    35341 aatagccagg caggtttttt atttttcaca atgaggtgaa tatgtcatca atatcacatg
    35401 gcgcaccaca aaagcgtcga attatcccta atcccggcct gtggttggcg atcattgccg
    35461 gaattattat tactcttctg cctctgggcg ataccttgcc cgtcgctgga caaaatatga
    35521 tcgccattct ggtgtttgcc attattgtct ggatcagcga agcgatggat tacaccgcca
    35581 gtgcaattgt tatttccgca ctgattatct ttatggttgg ttttgccccg gatatgaatc
    35641 acccggacac gatccttggc accgcgaaag cgctgaaaat gactctgtcg ggtttttcta
    35701 attctgctct ggcgctggtg gcagcagcaa tgtttatcgc ggcggcaatg accattaccg
    35761 ggctggataa acgcattgcg ctgtttacta tgtcgaaaat aggtgccagc agccgcagca
    35821 ttattatcgg tgccattgtg gtgaccatcg tcctgagcct ggtcgtaccc agtgccactg
    35881 cccgtacggc ttgcgtggtg ccgattatga tgggggtcat tgcggcattt aaggtggata
    35941 aacattcacg tttagcggcg tcaatgatga ttgttatcgc ccaggcgacc agtatctgga
    36001 acgttggtat tcaaacttcg gcggcgcaaa acctgctttc tatcggtttt atcaataaaa
    36061 ctttcggcgc cgggcattct gtcagctggc ttgactggct gttagcgggc gcgccctgga
    36121 gcctcaccat gtcggcgatc ctctatttcc tcgcacgtaa attgttgccg ccagaaacgg
    36181 aagctgttga aggggggagc gaggccatta aaaaagcact tgcagagtta gggccaacca
    36241 ctggtaaaga aaaacgtttg atcggtattt cgctattgtt actgctcttt tggtctactg
    36301 gcggaaaatt acacagtatt gataccacct ctgtcaccct tgccggactg gcgattatgt
    36361 tgctgccagg catcggtgta atgagctgga aagaggtcga aaaacgtgtg cagtggggca
    36421 cgttgcttat gtttggtatt ggtatcagcc tggggtccac gctgcttgat acgcaggcag
    36481 cttcatggat ggcgaactat gtggtgaaag gttttggtct cgatggcttg ccatcattag
    36541 ccatcttcgc gattctcgcc gctttcttga taattattca cctggggttt gccagtgcca
    36601 ctgcgctaac tgccgcattg ctgcccatcc tgattagcct gttaagcagt ctgccgccag
    36661 aactcggcgt taacccggtc ggcatgacca ttctgctcgc cttcagtgtc agttttggct
    36721 tcatcctgcc gattaacgcg ccacaaaata tggtgtgtat gggtacggat accttcacgc
    36781 cacgccagtt tacccgtgtg ggactgtacc tgacggtaat cggctacctg ttgttgctac
    36841 tgtttgccgc cacctggtgg aaaattttag gtctaatgtg aggaaaccaa cgatgtcatt
    36901 acaaattact gttgaaaaag tgcgttggtt agctgccggg ctgctggaac ttaatggctg
    36961 tgatgccgat atcgctcagg atgtggcgga acatatgatt gaagcggaac gttatggctt
    37021 tgccagccac ggcgtcacgc tgctaccgaa atatctggaa aatatcgctc gtggcgatgt
    37081 aaccgcgaat gcccgcccgg aatgcttaac aagtgaaggt aatttacaac gttttcatgc
    37141 ccacaatggc tttggtcaac acgccggaaa agtggcggta aatgctgcca tcgaacaagc
    37201 aaaacaccgc ggtttttgcc tgttaacgtt gtgccatgcg catcatttag ggcgtatggg
    37261 gcattacggg caaatggttg ctgatcaagg cctggcgatg ctggccatga gtaacgtcac
    37321 cgggcgtcct gcactggtcg ccccgtgggg cggtgcggaa ccgcgtatga ccactaaccc
    37381 tttctgcttt gcctggccgt tcagtgatgg gcgtccgccg attctggttg attttgccac
    37441 cagtagcatg gcgctaaata aagcacgggt aatgtcgtcc accgggaagc aggccgcgcc
    37501 gggacaattg attgatgcac aggggaatcc aagcaatgat cctggggtgt tattcagcaa
    37561 tcctccaggg gcgttactgc catttggtga gcataaagga tttggcctgg cactgatgat
    37621 tgaaatgatg gcggggatct tatctggtgg tgacacgatc gccgaagatc atcagacgga
    37681 tggatctgcg cataatcatc tttttgcgtt gattatcgat cctgatcaat tcaccgatct
    37741 ggcaggggag aaagggcagt tctttgcgga ttatctgtta gctacgcagc ctcaacctgg
    37801 cggcaatccg gtgatttacc ccggtatgcc agaagctgcc aaccgggaac gcaatgcgaa
    37861 gatgattacg atcccggtat cattctggtc ctgggtagtt gaacattacg cccgcaaagg
    37921 tatcgatttg gggttacatc ctcaggaatc agcattagct ggatgattct ggtcgataaa
    37981 cttgcggttg aatctgatgt ttgttgagtt tgtcatataa agtcttgcgc ggcagctgta
    38041 atagccgcgc tgtcaaactg acctggcctt ccgtttgtcg cagcatatct tcgattaact
    38101 ttttctcgaa agcatcaatg caggcggtaa gaccactctc ttcttgctga gtcattggca
    38161 ctggctggtg gtgggtcagt aaaccgagaa caaaacgctc tgcattgtgt ttaagttcac
    38221 gtacattgcc aggccaactt tgtgactgca accaggccag taacatggga gaaatatcag
    38281 gcagaggacg attatatttt tgtgccgcct gactggcgaa ccagtaaaac agttcaggga
    38341 tatcttctcg tcgctggcgt agaggtggaa tattcaggct aaccacattc agacgataat
    38401 agagatcgag acgaaacaga tgttcttcgc tgcgacgcag taaatcttcc ttggtcgctg
    38461 caataacacg gcaattcacc ggaattaatt gattggcacc taatcgttca atggttcttt
    38521 cctgcaatac acgtagcagt tttacctgca ttcctgatgg cattccctct atttcatcga
    38581 gaaataacgt accaccattt gcatgttcaa tttttccaat acgcttttta accgcaccgg
    38641 taaatgcgcc cgcctcatga ccaaatattt cgctttcaaa caacgtttct ggtaagcctg
    38701 cacagtttaa ggcaacaaac tgcccctgac ggcgggtact ccagtgatgc agcattctgg
    38761 cgacgacttc tttaccacaa cctgtttcac cataaataag gacatcggca ccggtatctg
    38821 cgacattaag gatcatttta cgcagttgtt gcatctgcgg actacgacca atcagcaccg
    38881 ggccattttc ctgttgcaga ttcgccagta attgctgatt ttctaaaacc aatcgccgtt
    38941 tttctaccgc tctggcaata atactcaata atttatctga gctacttggt ttctcaatga
    39001 aatcgaatgc accattacgc atggcatcca ccgccatttc cacgtcaccg tgtccggtaa
    39061 ttaaaataac cggaattttg ctatccattt cgttgatgta actgaggagc tctagtcctg
    39121 atattccagg taacctgaca tcggtcagta taataatgtt ggcctgactt tgaataaggg
    39181 gaatactgtg ttctgcatcg caacaggaaa taacacgata accagccagc gtgagcgttt
    39241 gttcacaggc aaaacgtaca tcgtcactat cctcaatata aactatagta atttcactgg
    39301 atttattatt catgttcttc tccccattca ggccaggtta atgtcattac tgcgccgcct
    39361 tcaggatgat tactggcgtg gagtgcgccg ttcgaatttc ggacaatttc gctgactatt
    39421 gccagtccca accccattcc tctacgttta gtggtaaaaa atggttcaaa tatgcgatcg
    39481 acaacttcag gtgcaaaacc gccaccgctg tctttaatgg taataataac tttgttattt
    39541 gcctggcgaa tggcgatatc cagtcgtttg taagagctac cttccataga atctaaggcg
    39601 ttgctaatta aattactgaa tatctgttca agaccgagct catcgcaatt gacaaataac
    39661 ggcatggatg gggcttttat tcgtcgctca atgttatttt tctccatgct gtgattaagt
    39721 aacgccacgg cgctatacat cactttgatg acatcggcag aacctttagg tacgcgatgg
    39781 cgcgaggcaa atgctttaag ttcggaaatg agctgcgtca tccgctcaat cactgaaata
    39841 atatgtttca gattctgttc aacctgcgga tacatctctt tttttagcat agtacgcgcg
    39901 ttatcagtaa gtgcgtgaat ggcggctaac ggttgatttt gttcatgggc aatttcggtt
    39961 gccatctgtc caagcgccgc cagtttgctg gactctgcca gttcactacg cgtaatttgt
    40021 aatacttgtt cagcttgact gcgctctttt atctcctgta ttaatttttg attgatcaat
    40081 tccagggcag atgtacgctc tttaacctgt ttttctaatg tttcattcgc aagtgttaat
    40141 aattgttgtg catgagatcg cattctccag tatttaataa ataacaaaaa cagcaggtaa
    40201 ataattagtg taatgactaa caaaatagcc catgaccagt acaggttatc taaggggacc
    40261 ataattgtca ttttccagtt aaattctgga atggctattt gttgcgtata atattgcggg
    40321 aataaacaga gttgttcttt tttatctttc aggaaagtaa aattgctcac ggtataacag
    40381 ggataataat ccgccggtaa aagattgtcg agactatatt ggcgggtaga ttgtagtttt
    40441 tgttttgcct gaacaggtaa cggttgcagt gttcgcattc gccatggcga ttttgaa
//
LOCUS       DAZFRE010000033        40244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFRE010000033 DAZFRE010000000
VERSION     DAZFRE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.033"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1055
                     /locus_tag="ACHKUI_002104"
     CDS             <1..1055
                     /locus_tag="ACHKUI_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO6598294.1"
                     /translation="RKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERILTD
                     RENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYAGL
                     SYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTDFF
                     GMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQSGD
                     GKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDFGL
                     RPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVLAH
                     GLNTDDQLAVGIVYQF"
     gene            1651..2502
                     /gene="hchA"
                     /locus_tag="ACHKUI_002105"
     CDS             1651..2502
                     /gene="hchA"
                     /locus_tag="ACHKUI_002105"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6598295.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2610..3968)
                     /gene="hprS"
                     /locus_tag="ACHKUI_002106"
     CDS             complement(2610..3968)
                     /gene="hprS"
                     /locus_tag="ACHKUI_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6598296.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDALSLNKEVENLLDYLEYLSDEKEIRF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(3968..4639)
                     /gene="hprR"
                     /locus_tag="ACHKUI_002107"
     CDS             complement(3968..4639)
                     /gene="hprR"
                     /locus_tag="ACHKUI_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO6598297.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4772..5185
                     /gene="hiuH"
                     /locus_tag="ACHKUI_002108"
     CDS             4772..5185
                     /gene="hiuH"
                     /locus_tag="ACHKUI_002108"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6598298.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5294..6298
                     /gene="msrP"
                     /locus_tag="ACHKUI_002109"
     CDS             5294..6298
                     /gene="msrP"
                     /locus_tag="ACHKUI_002109"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6598299.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSDAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6299..6934
                     /gene="msrQ"
                     /locus_tag="ACHKUI_002110"
     CDS             6299..6934
                     /gene="msrQ"
                     /locus_tag="ACHKUI_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6598300.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            7192..7842
                     /gene="zinT"
                     /locus_tag="ACHKUI_002111"
     CDS             7192..7842
                     /gene="zinT"
                     /locus_tag="ACHKUI_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO6598301.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(8512..8601)
                     /locus_tag="ACHKUI_002112"
     tRNA            complement(8512..8601)
                     /locus_tag="ACHKUI_002112"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8565..8567),aa:Ser,seq:cga)
     gene            8695..9492
                     /gene="mtfA"
                     /locus_tag="ACHKUI_002113"
     CDS             8695..9492
                     /gene="mtfA"
                     /locus_tag="ACHKUI_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6598302.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDPFSATNVH"
     gene            9593..9668
                     /locus_tag="ACHKUI_002114"
     tRNA            9593..9668
                     /locus_tag="ACHKUI_002114"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9626..9628,aa:Asn,seq:gtt)
     gene            9830..11092
                     /locus_tag="ACHKUI_002115"
     CDS             9830..11092
                     /locus_tag="ACHKUI_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6598303.1"
                     /translation="MSLTDAKIRILKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(11286..12590)
                     /gene="ybtS"
                     /locus_tag="ACHKUI_002116"
     CDS             complement(11286..12590)
                     /gene="ybtS"
                     /locus_tag="ACHKUI_002116"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO6598304.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(12618..13898)
                     /gene="ybtX"
                     /locus_tag="ACHKUI_002117"
     CDS             complement(12618..13898)
                     /gene="ybtX"
                     /locus_tag="ACHKUI_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO6598305.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(13891..15693)
                     /gene="ybtQ"
                     /locus_tag="ACHKUI_002118"
     CDS             complement(13891..15693)
                     /gene="ybtQ"
                     /locus_tag="ACHKUI_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO6598306.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            complement(15680..17392)
                     /gene="ybtP"
                     /locus_tag="ACHKUI_002119"
     CDS             complement(15680..17392)
                     /gene="ybtP"
                     /locus_tag="ACHKUI_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO6598307.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARRRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            17649..18608
                     /gene="ybtA"
                     /locus_tag="ACHKUI_002120"
     CDS             17649..18608
                     /gene="ybtA"
                     /locus_tag="ACHKUI_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO6598308.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            18799..24906
                     /gene="irp2"
                     /locus_tag="ACHKUI_002121"
     CDS             18799..24906
                     /gene="irp2"
                     /locus_tag="ACHKUI_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO6598309.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTTCDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYQYPQQLPPPLPVTYRDYLQQPAIQSLNA
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            24994..34485
                     /gene="irp1"
                     /locus_tag="ACHKUI_002122"
     CDS             24994..34485
                     /gene="irp1"
                     /locus_tag="ACHKUI_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO6598310.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGTRVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGQWLPLSPAEP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQTLSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQMPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            34482..35582
                     /gene="ybtU"
                     /locus_tag="ACHKUI_002123"
     CDS             34482..35582
                     /gene="ybtU"
                     /locus_tag="ACHKUI_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO6598311.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVEYDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETAGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            35579..36382
                     /gene="ybtT"
                     /locus_tag="ACHKUI_002124"
     CDS             35579..36382
                     /gene="ybtT"
                     /locus_tag="ACHKUI_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO6598312.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            36386..37963
                     /gene="ybtE"
                     /locus_tag="ACHKUI_002125"
     CDS             36386..37963
                     /gene="ybtE"
                     /locus_tag="ACHKUI_002125"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO6598313.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            38094..40115
                     /gene="fyuA"
                     /locus_tag="ACHKUI_002126"
     CDS             38094..40115
                     /gene="fyuA"
                     /locus_tag="ACHKUI_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO6598314.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         9208 a        11519 c        10596 g         8921 t
ORIGIN      
        1 aaagaaaagt tctggcaatg ctggtcccgg cgttattagt tgctggcgca gcaaatgcgg
       61 ctgaaattta taataaaaat ggcaataaag ttgagctata cggcaagatg gttggtgagc
      121 gcattctgac cgaccgtgaa aatggtgaaa aaggcgataa ttctcaagat acctcttatg
      181 cccgtgtagg cgtgaaaggt gaaactcaga tcaaccctga actgactggt tacggtcagt
      241 ttgaactaga cctggaagca tcaaacagac acaaccctga ccaaactcgt ttggcatatg
      301 ctggtttgag ttacaaagat tttggttcat tcgactacgg tcgtaatgtc ggtgtcgcgt
      361 atgatgcaga agcatttact gatatgtttg ttgagtgggg cggtgactca tgggctggta
      421 ctgacctgtt catgaccaac cgtaccaacg gtgttgcaac ctatcgtaac acagatttct
      481 ttggcatggt tgaaggtcta aattttgctc tgcaatatca gggtaaaaat gaaggtacag
      541 gcaactacaa agcaaatggc gatggccatg gtttatctgc aacttacact attgacggtt
      601 tctcatttgc tggtgcttat gcaaactctg accgtactga ttggcagtct ggtgatggta
      661 aaggcgaaag agccgaagtt tgggcgttgt caacaaaata tgacgctaac aatgtctatg
      721 ctgctgtgat gtacggtgaa tctcacaaca tgaattccga cgacggtgat gttgtgaaca
      781 aaacacaaaa cttcgaagct gttctgcaat atcagttcga ctttggtctg cgtccatcca
      841 tcggttactc ctattctaaa gcactggacg tagcaggtta caaagattct gatcgtctga
      901 actatattga aatcggtacc tggtattact tcaataagaa tatgaacgta tataccgctt
      961 atcagatcaa cctgcttgat aagagtgatt atgttctcgc tcatggactg aacaccgacg
     1021 accaactggc agttggtatc gtctaccagt tctaatcagc caacgcgccg aggcgtgtta
     1081 ctgtaaaaca taacaccagc ctgagttcgt ccctcatcga actcaggctt tttttattgg
     1141 catttcatct ccatctgcgg cacacgcaat tgccaaagca tctcgataca gccccttacc
     1201 ctgcccctcc tggcacaata tcctctacca gcaactcttc cagccaacac cccaaatgca
     1261 tttctttata aatttaatct gtaacaaaac cgcttcccat atagaaatat ttgcaaaaat
     1321 agtgcatgaa aaaagctttt tccctaccaa tccgggatat aaatattcat cagtaccaca
     1381 acattccaca gcactaaatc tctccccatc gcccccaaac ctctgataat ggtctaaaat
     1441 cattgagacc acttgcgacg acgttactca ataaggaaag tttggcgtga ataatattca
     1501 acaccagtgc agcatcatca ttacatactt atgcccgcca actaccgctt tcgatatatt
     1561 gactcaattt ttcagcctgg ttaaaaatag cgaccagctc agtcgcaaat atagtgacta
     1621 ccctaactaa gcaacaataa ggaatacact atgactgttc aaacaagtaa aaacccgcag
     1681 gtcgatattg ctgaagataa tgcgttcttc ccttcagaat attcgcttag ccaatatacg
     1741 agtcccgtct ctgatcttga tggcgtggac tatccaaaac cgtatcgcgg taaacataaa
     1801 attctggtga tcgccgcgga cgaacgttat ttgccgaccg ataacggaaa actgttctcg
     1861 accggtaacc atccgattga aacgttgctg ccgttgtatc atctccatgc tgcaggtttc
     1921 gaattcgaag tggcgaccat ttccggtctg atgaccaagt ttgaatactg ggctatgccg
     1981 cacaaagatg aaaaagtaat gccattcttt gaacagcata aatcgttatt ccgtaatccg
     2041 aagaaactcg cggatgttgt cgccagcctc aacgctgata gcgaatatgc agcaatcttt
     2101 gttcctggtg ggcatggcgc acttattggt ttaccagaaa gccaggacgt ggctgccgct
     2161 ttacagtggg caatcaaaaa tgaccgtttt gttatctccc tttgccacgg tccggcggct
     2221 tttctggcac ttcgccacag cgataaccca ctgaatggtt attctatttg cgcattccca
     2281 gacgccgcag acaaacaaac gccagatatt ggctatatgc cgggtcatct cacctggtac
     2341 ttcggcgaag agttgaagaa aatgggcatg aacatcatta atgacgacat caccgggcga
     2401 gtacataagg accgtaaact tctcaccggc gacagtcctt ttgcagcgaa tgcgttgggt
     2461 aaactggcgg cgcaggaaat gctggcagct tacgcgagtt aatccctgtt acattgtgat
     2521 gtaagcgcat aatcaatcgc agccacgtta atactcacga cgtggctgcg ttttttcacg
     2581 gttaacttat ggcgtactga agcccgatgt taatttcttt gcggtaacgt aattcggaac
     2641 acgttatgct tactgagata gtgatacgta gcacttcctc catgtaattc ggcaatcgct
     2701 ttgactaaag aaaggccaag tccctgacct acggaatgac gcgaattatc tccccgccaa
     2761 aatctacgga agagtttttc aggctcatta attttcgttc caggactggc gatatcaata
     2821 ttaagagaac cgttggcatc aagaaaactg gttatatgaa tacgcgattt ttctggtgaa
     2881 tatcgaatgg cgttaacaat aagattcgat aacattcgtt gcagtaaaat tttatccgca
     2941 aagatttgtt gattgcactc gaccttaaaa cgaatctctt tctcgtctga aagatattca
     3001 agatagtcca aaagattttc aacttcctta ttgagagaaa gcgcatccag tttcaccaaa
     3061 acattgtttt tgtctgccct ggcaagaaac agtatattct ctgttaaccg cgaaatattt
     3121 tccagctctt caatgtttcc ggcaattgtt ttttgatatt cagcgatact tctgatttga
     3181 ctgagcgtaa cctgattctg acccagtaat gcattaattg gcgttctaag ttcatgagcg
     3241 agatcgtcag caaattgact aaggcgctca aaatctttga ctaaagcatg atgcatttta
     3301 ttcaacgcct gcccaagagg ttttagttct ctcggtaatg cgctaacctc aaccggcact
     3361 cggctatcgt tataattcag cgcttccgtt acgccactca actttttgat ctctcgcaac
     3421 cccgtcctga ttaacaacgg acttaatact gagcacaata taatggcgac aatgcaaatt
     3481 ataatgctat taattttata ctgttcaagc atattatgtc tggctgaagc caatttagcc
     3541 acagtaaccg ttaatgatgg cgaaacttca tcaatgttaa ttcgtaaagc atctatctct
     3601 gtatcattaa taatgcttct gtaaatgcct gctgcgctga ttgtctcact agcaggtatg
     3661 ttatttaaca tgtcatcact gacatttgtc cggttaacaa ttttattgat gccatcacca
     3721 tgaatgatca aaatatcctg actaacatcc atcatccggt taaagtacac aggtaacgta
     3781 tctggattta ccccatcaat taacaactgc ttgatctgcg ctgtccggtt aatgagtgtt
     3841 gtatcatcgc gccatttcaa ctcacttgcc aggccattat agagagtcca tacaattcca
     3901 gcgccagcaa cagacagcag caatataaaa agcaaggtta aacggacggt tatagataat
     3961 cttttcatta tttttttacc gctacgaatg aatagcccat cccccggatt gtggcgatta
     4021 gcttttcagg aaaaggatca tcaacttttg cgcggagcct gcgaatggcg acatccaccg
     4081 tattggtatc actatcaaag ttgattcccc aaatttcact cgcaataacc gttctgggta
     4141 taatctcgcc agctctggag gccagtagcc aaagtaactg aaactccttg cgcgtcagtg
     4201 taatactgat attgtccctg ctcacacttt gactgacaga gtccattttt aagccactga
     4261 tttctaatgt tgaattcaaa gcgtgatgtt gccttaattg tgcccgaacc cttgccagca
     4321 actcagaaaa tgaaaaaggt tttaccagat aatcatttgc tccactgtcc agccctctga
     4381 ctctgtcatc gacagaatcc cttgctgtaa ggcaaataac aggagtttgc tttgccgttc
     4441 ttaacgtttg taagatctgc cagccatcca tacccggaag cataatatcc agaatgatca
     4501 atgcataatc atccttcagc gcaagataaa gcccatctct gccatcagaa acggcatcaa
     4561 tgacataacc cgcttcggaa agcccctgcg ttacccattc ctgggtcctt tgattatctt
     4621 caataagtag aatcttcatg aaaataatat gccataaatc atatgttata gaaacaacct
     4681 ggttcattac aaaattgtaa tgctgctgta aggttaccct ggacgttttt tcgctatcct
     4741 caaaactcat tcacatgaca aggatataaa catgttaaag cgttatttag tactctcggt
     4801 agcaacggca gcattttcat taccttcttt ggtttatgcc gcacaacaaa acattcttag
     4861 cgtgcacatt ttgaaccagc aaacaggaaa acccgctgcc gacgtgacag tcactcttga
     4921 aaagaaagcg gataacggct ggttacaact taataccgcc aaaacagata aggatggacg
     4981 aattaaggca ctgtggcccg agcaaactgc gactactggc gattaccgtg tcgtatttaa
     5041 aaccggggac tatttcaaga aacaaaatct tgaaagtttc ttccctgaga tccccgttga
     5101 atttcatatt aataaagtga acgagcatta tcatgtgcct ttattactca gccaatatgg
     5161 gtattcaacc tatcgtggca gttaatgcgt aaatatgtaa aagctgatct ctgctactgt
     5221 tagggaggtg actccctgac agattaacag taaacggctc ttgttggcta acgacaaaaa
     5281 agtgtgatgg cttatgaaaa agaatcaatt tttaaaagaa tcagatgtta cggccgagtc
     5341 ggtattcttt atgaagcgtc ggcaggtgtt aaaagcactg ggcatcagcg cagctgcatt
     5401 ttctttgcct cacgctgcgc atgccgatct gcttagctgg tttaaaggga acgatcgtcc
     5461 gcccgccccc gccggaaaac cgctggagtt cagcaagcct gccgcctggc aaaataacct
     5521 gccactgacg ccagcagata aagtctccgg ttataacaac ttctatgaat tcgggctgga
     5581 taaagccgat cccgcggcta atgctggtag cctgaaaacc gatccatgga cactgaaaat
     5641 cagcggcgaa gtggcaaaac cattgaccct cgatcatgat gatttaaccc gtcgcttccc
     5701 gctggaagag cgtatttatc gtatgcgctg cgtggaagcg tggtcgatgg tggtgccgtg
     5761 gattggtttt ccgctacaca aattgctggc gcttgccgaa cccaccagcg atgcgaagta
     5821 tgtcgctttc gaaacgattt atgcgccgga acagatgcca ggccagcagg accgctttat
     5881 cggcggcggg ctgaaatatc cttatgtcga aggattgcgt ctcgacgaag caatgcatcc
     5941 gctcacactg atgaccgtag gtgtttatgg caaggcgtta ccaccgcaaa atggcgcacc
     6001 ggtgcgactg attgtgccgt ggaaatatgg ctttaaaggg attaaatcga tcgtcagtat
     6061 taagctgacc cgcgagcgtc cgccaaccac ctggaatctg gcagcgcctg acgaatacgg
     6121 tttttacgcc aacgttaatc cgcatgttga tcacccgcgc tggtcacagg ctaccgaacg
     6181 atttattggt tcaggcggca ttctcgatgt tcagcgccag ccaacactac tgtttaatgg
     6241 ttacgccgac caagtggcat cgctgtatcg tggcctggat ttgcgggaga atttctaaat
     6301 gcgtctgaca gcaaaacagg tgatatggct gaaagtttgc ctgcatcttg ccggattgtt
     6361 gccgtttctc tggctggtct gggcgatcaa tcacggtgga ctgggtgccg atccggtgaa
     6421 agatattcag cattttactg gtcgcactgc actgaaattt ttgcttgcgg ccttgttaat
     6481 cacacctctg gcacgctatg caaaacagcc gttattgata cgcactcgcc gcctgttagg
     6541 cttatggtgc tttgcctggg cgacactgca tttaaccagt tacgcattgc tggagttggg
     6601 cgtgaataat ctggcgttat taggtaagga gttaattacc cggccttatt taacgttagg
     6661 aattatcagc tgggttattt tgcttgcttt agcgttcact tcaacccagt cgatgcagcg
     6721 aaaactcggc aaacactggc aacagttgca taacttcgtc tatcttgtcg cgatcctggc
     6781 cccaatacat tatctgtggt cagtgaagat tatttcaccg cagccactca tctacgctgg
     6841 gctggctgta ctgcttttag ccttacggta taagaagttg ctttcccttt tcaaccggtt
     6901 acgcaagcag gcccacaaca aattatctct ataacttcat attgccgaca aacttcgcgt
     6961 tatcccggcc ggatgcggcg cgagcgccct atccggccca cacagatcgc atcaagttat
     7021 gtttcgtttg tcatcaacct ttcatcacca gaataattta ttttatttca ataagatagt
     7081 taaatacacc tgagaatacc atgctctcat ttcctaatga gttgttgcat tttttcatat
     7141 gttacaatat aacattacac atcatgtaca ttaactctgg aggaaattgt tttggcgatt
     7201 cgtcttcata aactggctgt tgctttaggt gtctttattg ttagcgctcc tgccttttcg
     7261 catggtcatc actcacacgg caaaccctta acagaggtcg aacaaaaagc tgctaatggt
     7321 gtttttgatg ataccaatgt acaaaaccga acgctcagtg actgggatgg agtctggcaa
     7381 tccgtttacc ctttactgca aagtggcaaa cttgaccccg tctttcagaa gaaagcggat
     7441 gcagataaaa ccaaaacatt tgctgaaatt aaagattatt atcgcaaagg ttatgcgaca
     7501 gatatcgaaa tgattggcat tgaggacggc attgtcgaat tccatagaaa taatgaaaca
     7561 acatcctgta aatatgatta cgatggatac aaaatactca cctataaatc aggcaagaaa
     7621 ggcgttcgtt acttattcga atgtaaagat cctgaaagca aagcccctaa atatatacaa
     7681 tttagcgatc atataattgc accacgaaaa tcatctcatt ttcacatttt tatgggtaat
     7741 gattcccaac agtctttatt gaatgaaatg gaaaactggc caacgtatta tccctatcag
     7801 ttgagtagcg aagaagtggt cgaggagatg atgtctcatt gatttttttg cgctgacttc
     7861 agttatttaa cagaacagtg cctggtagta acaacaataa tttaccaaga caaataagta
     7921 agacaaactc agacacgctc atacttgagt cgtgtctttt ttattatgtc tgtagaaaaa
     7981 aactgagaaa aaggcgaaca ataaatacac aactcagcca tacagactgg cagcgcaaat
     8041 gtttccagga gcaagtcatc tgtcatggta aagctaacca gaaatatagg ttaattttgg
     8101 tgtttaaacc ttttttactt gttagtatga tacaaagact ttcaaaaaag ctgggaaatt
     8161 caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg tcgcgttcct
     8221 tttttggctc aattattgac cccttctcta cgcaacttca gttcccgcca gcaactttgc
     8281 ggcagtttca taggatcaat gtctaaaaga ataatggtga ccgataagaa aacgactgaa
     8341 taactgtaga ttttcgctcg aaaccttcct gtcagaccca tagcgaatca agtgctgaat
     8401 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt ctacgattgt
     8461 tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa gtggcggaga
     8521 gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga ccggtccgtt
     8581 cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa tttggcattt
     8641 taacagtccc tgtccgtgca attttgttca agtgacgagt ttgcgagcaa aacgatgatt
     8701 aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg gcaggaagca
     8761 ctatcgatcc cccttttaac gtgtctgaca gaacaggaac aaagcaaatt ggtcgctctt
     8821 gccgaacgtt ttttacagca aaaacggctt gttcctttac agggctttga gctgaattca
     8881 ttaagaagct gccggatagc acttctattt tgcctgcccg ttctggagtt aggactggaa
     8941 tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt cgatgatgaa
     9001 tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg tcagagctgg
     9061 cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga tgcttctggt
     9121 tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc
     9181 agcggagttc cctttatttc gttgcgtgag gttgctggct gggaacatga tcttcatgct
     9241 gcaatgaaca acattcagga agaaatcgaa ttagttggtg agaatgcggc gagcattgat
     9301 gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac tttctgaata tttctttagc
     9361 gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg tcaattttat
     9421 caacaagatc ctttgcagag actgcatcac gctaatgata cagacccgtt ttcggcgacg
     9481 aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatggc ttatgaaatt
     9541 tagtgttgac agacaaggta ccgctaagta atatgcgccc cgttctcacg attcctctgt
     9601 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     9661 aggagccaat tttctgtttt catgcatcct tgcgaatctt tatctgatgt tgattcaaca
     9721 ggttagtgtg aaaacccttc tcggtgcatt ttgattttac cctacgcatc gggaaaaatt
     9781 ggtggtcaaa tctggggtca ggttagttcg ataatggagt gacccccata tatcccttac
     9841 cgacgcaaaa atccgcatcc tcaagccttc tgataaaccc tttaaagtct ccgattctca
     9901 cggtctgtat ctgcgggtca agccgggtgg ctcccgccac tggtatctca aataccgtat
     9961 tagcggtaaa gaatcccgca ttgcgctggg tacctatcca gccatctccc tgtctgatgc
    10021 gcgacagcaa cgtgaaggta tccgtaaaat gctggcgctg aatatcaacc cggtacagca
    10081 gcgggctgct gtacgtggct cacgaacacc ggagaaagtt tttaaaaacg tggcgctggc
    10141 gtggcataaa agtaacagga aatggtcgca gaacaccgcc gaccgtctgc ttgccagcct
    10201 gaacaatcac atctttccgg tcatcgggaa cctacctgta tcagaactta aaccccgtca
    10261 tttcattgac ctgctgaaag ggatcgagga aaaaggtctg ctggaggttg cgtcccgcac
    10321 acggcagcac ctgagtaaca taatgcgcca tgcggtccat caggagttaa tcgatacgaa
    10381 ccctgcagca aaccttggcg gcgtgaccac acctcctgtc agacggcact atcctgccct
    10441 gccgctggag cggctgcctg aactgcttga acgtattggg gcatatcatc agggccgtga
    10501 actgacccgg catgccgttc tgctgatgct gcatgtgttc attcgctcca gtgaactgcg
    10561 tttcgcccgc tggtcagaga ttgatttcac aaaccgagtc tggacgatac ccgcgacgcg
    10621 agaacccatt attggcgtgc gttattccgg ccgcggggca aaaatgcgaa tgccgcatat
    10681 cgtccccctc tcagaacagt ccatcgccat tctgaaacag attaaggata tcaccggtaa
    10741 taatgaactg atcttccccg gcgaccataa cccgtataag ccaatgtgtg aaaacacggt
    10801 caataaggca ctgcgggtga tgggttacga cacgaaaaag gatatctgcg gtcacggctt
    10861 ccgggcaatg gcatgcagtg cgctgatgga atcgggttta tgggcaaagg acgcagtaga
    10921 acgccagatg agtcatcagg agcgcaatac cgtgcgcatg gcttatattc ataaggcaga
    10981 gcacctggaa gcccgcaaag cgatgatgca gtggtggtcg gattatctgg aagcatgccg
    11041 agaatcttat gcaccgcctt atacaattgg taaaaataag tttatcccat agtatactaa
    11101 cgaatcccac actctggcaa attaccacca cctccacaag aggtggttct atatagactt
    11161 ttatcaactt catgacgaaa aacacctatt ttatcccaaa agaagacaat agagtcacag
    11221 aggaatcaaa gagcgactca attgtctttg gacagtctgg ttgtgaggtt agaaaacagt
    11281 tactcctaca ccattaaata gggcgcaatg ctcgctaatt tctcccgggt ttctgtcagt
    11341 tcgcgttccg gcgtagattg cgcgatgatt cccgcccccg cctgtatcca gcagcgctgg
    11401 ctatcctgaa atacggaacg cagaactagc gccgcatcaa agcgcgtatc gtccagcagc
    11461 aggattgcgc cggaataaag ctctcgcggc gtcttctcaa tttgcataat cgcgttcaga
    11521 gcagcatttt tagggatacc tgaggcggta atcgacggaa acagcacggt aaacgcatcc
    11581 caggcatcct tgttttccgc aagctgaccg ctcacgccag accccagatg ctgaacgctg
    11641 ccgcgctggc gaaccgacat taaatcttca accaccacgc tgcccggctg gcaaacggcc
    11701 tccagttcag caatagcttc tttgacagaa aggatatgct caagcacctc tttactgtcg
    11761 tgcagcagtt ctgcctcttt cgccttatta tgctccgggt ttcccatgcg atcgcgggtg
    11821 cccgcaagcg gttcagtgac cactttattg cccgtcactg acatcaccag ttccgggcta
    11881 aagcccagcg cttcgcgtcc ttcctgacgg aacataaacg agcgcacagg cgtgtttgcc
    11941 tgccgcccgt ataacagcgt ggcgggcata tcaatccgcg atggcagggg aatggcgcgc
    12001 gagacaatca ctttgacata ctcgccacgg cggatctccg caacggcgcg cgcaacctgt
    12061 tgtttatatg cctcaccatt gagggcggta tccaccgcca gtggtgcgtt ctgcgtcgtt
    12121 gtaccggcct cttttaccca ctcgcacaaa cgtcggcacc cgtcggcgga gtccgcataa
    12181 acggtgacat ttcccttttc aaaaataagt tcttcacggg gaacggttaa cgtcagcagc
    12241 ggccactccc cggcgttaaa ggcaatcccc cgcgcatgcg ccgcaaagtt gaacccaacc
    12301 tgtccatata cccgacgtcc tttcacctga ggatgcgcca taaaacgacg cgcgcagtcg
    12361 gcaatggaat ccaccgccca tttttgctcc ccggcatcat caataaatgt cgcctgcgtt
    12421 ccgtcggcat taatgtgcag ccgtgcctgg caccctttgc ctaaatacca acagggttga
    12481 cgctcataga cataacactc ttcttctgcg aactggcgta aaacgccaga aatcgtcggt
    12541 agccattgtt cctctggtaa cgccagatgt agaaattcac tgattttcat cccttacctc
    12601 tctgtgttat tcgcggatca atcaattgcg cttgtcatca gttctcttgc cgaatgcagc
    12661 cggatataag caacccacgc cgccagcaac gtgaatgccc cggcaaacag gaagcaggcc
    12721 gcatagccaa aatgttgagc aacaacgcca ccgataacgc cagccagaat ggcgattgcc
    12781 gcgtcagtac attgaaagag ggtgaagtcg acaccggcct gcaaaggcga tgaaagcgac
    12841 atcagcgtgg cgtaaagaca gaccagcgcg caggccagcg aaatggactg gacaatgacc
    12901 agacactgga gaatctgcag cagcagatga cccggcgcca tcatgagcgt catcaccacc
    12961 gccagcagcg cgatcccctg gacgccataa gccgtcaaca gcgctctgcc gggtgaggtg
    13021 tatttcatca gcaatccgcc ggccagcgtt cctgctatgc ccgctgcaat attgccgccg
    13081 ctgaacagcg cgcccaatgc ggacatgctc aacccatgat ccaacaacag cggtgccaga
    13141 agaggcagca caaaccgcat gcctgaattc agcatcaata ccagcagtaa gcccaggcgc
    13201 gcctgcttcc tccttagcgc ataacctaac gccggaacat gcgggatagt cgctgttcgc
    13261 gtcggctccg taatgcgcca cagcgggagt gacagcgcca taatcagcac cgccatcatc
    13321 agcatggcga caggccagcc ggatgccgcc gacagcatca ggaacacccc gccgccgcac
    13381 atcattccca gatagctgcc gccgacctgc acgctgtttc cccagccgta acccgtgcgg
    13441 gtcagttgat cgacacaaaa gccgtcgctg gcgatatcaa tggtggaggc gaccgtgcct
    13501 gccaccataa acagaacaaa taacgcgacg atttgcgtat cggggaatcc cccttctcgc
    13561 ccaaaccagc caatcgctgc ggcaatagtc aggatcgccg ctagcgcgac ctgaccacgc
    13621 aggattaaca tgcgggaacg gcgttcctgg ctaccgggcg gaagccgcca gcgctcgatc
    13681 cacggcgccc aaataaactt cagcgcccag ggcagcataa acagggttgt cgcaccggca
    13741 agcgccagcg aaccgccagc agcgcgcacc agcgcgggta aggactgcat agacagcgcc
    13801 gataccagac tctgcgtcac atagacgccg gcaatagcaa aaatcacccg cccggtcgtc
    13861 aacgtcagag gtttcacatt cgactgaaca tcactcatgc acccactctc cagacgcgga
    13921 acccccgtcg tcgcgccaca cgcgcgcggc catttgcgcc tgccacaacg cctgataacg
    13981 tccatgatgt gagagcaatt gcgcatgagt accctgctca accacctgtc cctcttccat
    14041 caccagaatg tttccggccc cggcgatggt ggataaacgg tgagcgatga taatcaccgt
    14101 ccggttgtga accaggttat cgatcgcttt ttgcaccgcc agctcgcttt caatatccag
    14161 cgcggcagtc ggttcatcga gaatgacgac cggcgcgttt ttcaataacg ctctggcaat
    14221 ggaaatccgc tggcgctcgc cgcccgatag ctggccgccc atctctccca ttggcgtcag
    14281 ccagccttgc ggaagacggg aaataaactc aaggcactgc gccgcgcggg cggcctcttc
    14341 tacctcctgc cgcgtcgcct gtgggcgcgc gatacggata ttcgccagca gcgtgtcatc
    14401 aaacagccag acatcctgga aaacaacaga aatcaggcta ttgagctgtt ccggcgtcag
    14461 gcggcgaata tcgacgccgc caatagaaat ctgcccttgc tgcggatcgg catagcgcat
    14521 taacagtttg gtgaccgtag ttttgcctgc gccggaggca cccaccagcg cgctcatact
    14581 ggctgccggg aacgtcaaag aaacatgatt aagcgcgtgg ccgtcacctt cttcgtagcg
    14641 atagctgacg ttgtcaaagc ggatatcgta acgttccggc atctcgcttt gctctgcgac
    14701 gggaagcggt gctatcgcca taaactgctc aatccgttgc agggcgctgg cgatcagttc
    14761 cacaaccgag gtgtagctga taaacatcgc catcggttct gcgaagcgca taatcatcgc
    14821 gacggcggca atcaaaaagg cgaggttcag agtgcccgtc actacccaga cgatcccgga
    14881 taacaccacc acctgtaggc ccaactccac gacgctggcg atcagcatcg tcgcgccagc
    14941 gccctgacgg tgagtgcggg tctgtaagtt ttccagcgca ttgaaatgcg ccagcagcgc
    15001 ccggctttta tcggcatcgc tgccgcaggt gcgcaatacc atcatcccct gagcaaattc
    15061 aacgatatcg ccgctcagac gctggtgcgc ttcccccagc gtctgcattt gtcgccgcat
    15121 cgccgggcgg cgccagtaat aaaacggcac cagcagaggg aagatcagca acatcaccag
    15181 ccccagccgc cagtctatcc acaatgtcgc cagcgacgcc gtcagcggcg tgacaatggt
    15241 gagcagcaaa atattggcta tcgcaataac ataattgagg ttttcatcca cgctgcccag
    15301 cagcaaggcg ttcatttcac ccgccctgcc gcgctggagc ttctccagcg gcacgcggcg
    15361 taactgctcg ccaagtcgca ggcgcaactc atgggtggcc tgcgccagat gaccacggta
    15421 ttcaaagccc aggccatacc agcgtagcac cagcgtcaca attgcggcga cgctgaaggc
    15481 catagcccag ttaagaagtt gcggcgcgtc tccccgtaac agcgcatcaa tgatcggata
    15541 aagacaggca aaggcgatcc cctgcatgaa tgccgccagc aacagcgcca gcatactgcg
    15601 tcgcagcatc ctggcctgac tgccaacgct ggagataagc tggcgccaga tgacgcgcca
    15661 ggccaggtta tccgcaggat tattgtcttt cattttccac cacctcttcc tgtgttccac
    15721 ccggcaccca atgctgcgcc tgctgatagt gttgccagag ccgttgatac aggccgccct
    15781 gcgccaacag ttgcgtatgg ttccccattt ccctgagctg tccgtcggaa aacagcaata
    15841 tcacatcggc ctgagtcacc attgagagac gatgcgcgac catgatgacc gtccggccac
    15901 gcatggccgc cgcgagcgcc ttgataagcg ccgcttcgtt ttccgggtca gcaaacgccg
    15961 tcgcctcatc gagcaccagg atggggcgat cctgcaaaag cgcccgggcg atagtaatgc
    16021 gctgccgctg gccgccggag agaaataccc cacgttcccc gactcgagtg ttgtaacctt
    16081 ctggcagagc gctaataaaa tcatgggcct gcgccactct ggccgccgct ataaccgcct
    16141 ccagcggcgt atccggcgcg cccagacgaa tgttattggc tatcgtgtcg gcaaaaagga
    16201 agttgtcctg aaacacaaac gagagttgct tcatcagggt gtccgtctgc atatcacgca
    16261 gatccacgcc gccaatacgg atatggcctt tgtccgggtc ggcgtaacgc agcagcaagc
    16321 gcgccacggt gctttttccg gcgccgcttg gcccgaccag cgccacaatt tgcccggcag
    16381 gcacatgaaa gctcacctcc tgcaacgcgg cgccagtacg cgcttgcgga taatgaaagc
    16441 tcacctgctc aaaggtaatg ctcgcctctt gcggctgctg gtcagactgc ggcagcgata
    16501 actccggcat cgcgagaacc tgataaatac gctgaatgct taaacgcgtt tgcgcgacca
    16561 ggttattgag catcatcatt ggcattacgg cttcggccat tccgctgccc agcagtaaaa
    16621 cggccaccca cgcgataaaa tcgaaactgc catagtgcaa caggccgtat cccgaccaga
    16681 tcaggacaaa cagggtcggg agaggattca gaatcgagaa ggaaaaacgc gctgaaaaac
    16741 cggctttgcg ataccaggtt ttgagcacat cgacccactc ttcaagggcg cgttgatagc
    16801 gcaaaaaact ggtgctgccg ctatcaaacg ttctcaccac gggcatggcc tgcacgaact
    16861 caataaccgc cgcgctaacc tgctcgcgcg ccttatgata acgctgagcc atatcttccg
    16921 aacgccggcg ggcgagcacg agaacaaccg atccaaacgc cagtaccccc agcgtcgcga
    16981 ttgccagccg ccagtccagc cagaacaaga taacgattgt cgccagcggc atgatgatcg
    17041 cgcgggcata gagcggcgtg ctgtcggcca caaaaatatg taacgacttc acgtcatcca
    17101 gcatcacttt cgccactgac ccgctgccca tttgctgtaa cacgccaggc ggaagctgca
    17161 atgctttacg ggctaacctg ctgcgcagaa tgttctccag atgaaaagcc gcgtaatgag
    17221 aggtgttgaa cgcctgtaag cgcagaacaa aagttaagac cacgcagccg atcacgccgc
    17281 ccagcggcca ggccggaata gcgtcaggcg tcgcgcgaat gtcacgcagg ctccacgcca
    17341 gaaaaaggag cgcccccagg ctggttaatg ccgccagccc cgccagcgcc atagctaaaa
    17401 taaccttcct gcgaaccggt gtaatcacct gccagagagg aaatcgggac agcgattcag
    17461 tattggatga ttgagatgac atgggagtaa ctgaatttcc tgatgaattt ttacggacgc
    17521 caaaaacgac aattcagggt ggcgttctga gaattaatgt gaataataac cattatcaat
    17581 aactcatcta ccccattcgg ttctatttat acccgcattg gtctaagcca cagggagata
    17641 accaggtcat gatggagtca ccgcaaacgc aatctgaaat ctctattcac cagttggtgg
    17701 tcgggaaacc tgcaaacgat ggcaatattc ctgctcagtg tgaactgctg cgctgttcgt
    17761 tgcaggaagg tatggatatt ttgctctgga ggggccattt tgcgcggcct gagacgttgc
    17821 agctacacga cgatctgggc aggattaact tctcgtgcat cctggaaggc acatcgcgct
    17881 ttgcgattca gggactccgg cgccacaccg actgggagct tgcgcgcaat cgccattaca
    17941 ttacccatac gccagactgt cgcggcagcg cctcctattg tggccgattc gagagcatta
    18001 ccctctcctt cagccctgaa accctcgcgc tgtgggtgcc ggatattagc gcggtcatta
    18061 agaataagat cgactcccac tgctgttgcc agcagcagcg ttgtaatgct gaaacccact
    18121 taacggctca ggccttgcgt cacgcgctga tgcgcatgca cggcggcttc agtcatgaac
    18181 aaaagccgtc gacgctatgg ttgcaggggc aaagtctggt catgctcagt ctggtgctgg
    18241 atgaacatcg cgaagacgcc agttgcctct cctgccactt caacccgatg gaacgccaga
    18301 aactgctgcg ggcgaaggat ctgctgctgg ctgatctgac gcaagcgccg ggcgtcgcgg
    18361 agttggccag agagtccggt ctgagcgttc tcaaaattaa gcgaggcttc cgcgttctgt
    18421 tcaacaacag cgtctatggc ctgtttcagg ctgaacgaat gcaggaagcc agaagacgcc
    18481 tggccaacgg caatacctcc gtcatgacgg tggcggccga tctgggctat gccaacgcca
    18541 gccatttctc tgccgccttc cagaaacagt ttggcgtaac tccttcgacc tttaaacgcg
    18601 ggatgtaacc ggggtcgcgc ccccctaaac tcctccctga cagaggcccg gataccccat
    18661 ccgggcctct gttctttccg cctgttacca acccgaaacg ggcataaata aacccgtttc
    18721 gggtagcatc caattgttaa taattattat tctcatatga gtaatgcttt tcggtaagac
    18781 gtgccatcag gaggaagaat gatttctggc gcaccatctc aggattcgct gttaccggac
    18841 aaccgccacg cggctgatta ccaacaatta cgcgagcggc tcatacagga actgaattta
    18901 acgccgcagc agttacatga agagagcaac ctgatccagg ccggcctgga ttccataaga
    18961 ttgatgagat ggttacactg gtttcgtaaa aatggctacc gccttaccct tcgcgagctg
    19021 tatgccgccc ccacgctggc ggcatggaac cagttaatgc tcagccggtc gccggagaac
    19081 gcggaagaag aaacgccgcc cgacgaatca tcctggccga acatgaccga aagtaccccc
    19141 ttcccattga cgccagtaca gcacgcctac ctgacgggcc gcatgccggg gcagacgctt
    19201 ggcggcgtgg gttgccacct gtatcaggag tttgaaggcc attgtctgac ggcgtcgcag
    19261 ctggagcagg ccatcacgac cttgctgcaa cgccacccaa tgctgcatat cgcctttcgc
    19321 cccgacgggc agcaggtctg gctaccgcaa ccttactgga acggcgtcac cgttcatgat
    19381 ttacgccata acgacgctga aagccgccag gcctatctgg acgcactgcg ccagcgcctg
    19441 agccaccgtc ttttacgcgt ggaaatcggc gaaacgtttg attttcagct gacgctcttg
    19501 ccggacaatc gccaccgcct ccatgtcaat attgacctgc tgattatgga tgcctccagc
    19561 tttacgcttt tcttcgatga gcttaacgcc ctgctggccg gagaatcgct gccggctatc
    19621 gacacccgct atgatttccg ctcgtatttg ctgcaccaac agaagatcaa tcaaccactg
    19681 agagacgacg cgcgcgctta ctggctggcg aaagcatcga cgcttccccc cgcgcccgtc
    19741 ttgccgctgg cctgcgaacc cgccacgcta cgtgaagtcc gtaatacccg acgccgcatg
    19801 attgttccgg caacacgctg gcacgccttt agcaaccggg ccggcgagta tggcgtgacg
    19861 ccgacaatgg cgctggcgac ctgtttttct gccgtgctgg ctcgctgggg cggcctgacg
    19921 cgtctgctgc ttaacatcac cttattcgac cgccagccgc tgcacccggc ggttggcgcg
    19981 atgcttgccg acttcaccaa tattcttctg ctggacacca cctgcgatgg cgataccgtc
    20041 agcaacctgg cgcgtaaaaa ccagctcacg tttacggagg actgggagca tcgccactgg
    20101 tccggcgtcg aattactgcg tgaactcaaa cgccagcagc gctaccccca cggcgccccg
    20161 gtggtattta ccagcaatct ggggcgttcc ctctacagca gccgcgcaga atcgccgttg
    20221 ggcgagccgg aatggggcat ctcgcaaacg ccgcaggtct ggatagatca tctggcgttc
    20281 gagcatcacg gcgaggtctg gctacaatgg gacagcaacg acgcgctgtt ccctccggcg
    20341 ttagtcgaaa cattgttcga cgcctactgc cagttgatta accaactctg cgatgacgaa
    20401 agcgcctggc aaaagccgtt cgcagatatg atgcccgcca gccagcgcgc gatacgcgaa
    20461 cgggtcaacg ccaccggcgc ccccattccc gaaggcttgc tgcatgaagg cattttccgt
    20521 atcgctctgc aacagccaca ggcgctggcg gtaacggaca tgcgttatca gtggaattat
    20581 catgagctga cagactatgc ccgccgttgc gcgggcaggt taatcgagtg cggggttcag
    20641 cccggcgata atgtggctat cacgatgtcg aaaggcgcag gacaacttgt tgcggttctg
    20701 gccgtcctgc tggccggggc ggtttacgtt ccggtttcgc tggatcagcc tgccgcacgg
    20761 cgcgagaaaa tctacgctga cgccagcgtc cggctggtgc tcatttgtca gcacgacgcc
    20821 agcgccgggt cagacgatat tcccgtcctt gcctggcagc aggccattga ggcggagccg
    20881 atcgccaacc cggtagtacg cgcccccacg caaccggcct acattatcta cacctccggc
    20941 tctaccggta cgccgaaagg ggtagtcatt tctcaccggg gagcgcttaa cacctgttgc
    21001 gatatcaata cccgctatca ggttggcccg catgacaggg tgctggccct ctccgcccta
    21061 cattttgatt tatcggttta cgacattttt ggcgtactgc gcgcgggcgg cgcgctggtg
    21121 atggtgatgg aaaatcaacg gcgcgatcct cacgcatggt gtgagctgat ccagcgccat
    21181 caggtcacgc tctggaacag cgtcccggcg ctgttcgata tgctgctgac ctggtgtgaa
    21241 ggtttcgccg acgccacgcc ggaaaacctg cgcgcagtga tgctttccgg cgactggatc
    21301 gggcttgacc tccccgcccg ttatcgggcc ttccggccac aaggacaatt tatcgcgatg
    21361 ggcggtgcca ccgaggcgtc tatctggtct aacgcctgcg aaattcacga cgtccccgcc
    21421 cactggcgct ccatccctta cggttttccg ctaaccaacc aacgctaccg ggtggtggat
    21481 gaacagggcc gggactgccc tgactgggtg ccgggtgaat tatggattgg cggcattggg
    21541 gtcgcggaag gctatttcaa cgatcccctg cgtagcgagc agcaattttt gacgctcccg
    21601 gacgagcgct ggtatcgcac cggcgatctc ggctgctact ggccagatgg cacaatcgag
    21661 ttcctcggtc gtcgcgacaa gcaggtcaaa gtcggaggat atcgcatcga gctgggcgaa
    21721 atcgaaagcg cgctcagcca gctggcgggg gtgaaacaag caaccgttct ggcgatcggc
    21781 gaaaaagaaa aaacgctggc ggcatacgtt gttcctcagg gcgaggcttt ttgcgttacc
    21841 gatcatcgga acccggcact gccgcaggcg tggcacacgc ttgcgggaac gttgccctgt
    21901 tgcgccatct cgccagagat ctccgcagaa caggtagccg atttccttca gcatcgcctg
    21961 ctaaaactga agccgggtca caccgctggc gccgatcctc tccccctgat gaactcactc
    22021 gctatccagc cgcgctggca ggccgtggtg gaacgctggt tagcatttct ggtgacacaa
    22081 cggcgactga agcccgctgc tgaaggttat caggtctgcg ctggtgaaga acgcgaggat
    22141 gagcacccgc acttcagcgg acatgattta acgttatcgc aaattcttcg cggtgcccgt
    22201 aacgaactgt cgttactgaa cgacgcgcag tggtcgccgg aaagcctggc ctttaaccat
    22261 ccggccagcg ccccgtatat tcaggaactg gcgacaattt gccaacagct tgcacagcgc
    22321 ttacagcgcc cggtgcgcct gcttgaggtg ggaacccgca ctggccgcgc cgcagaatcg
    22381 ctgttagcac agctcaacgc cggacagatt gagtatgtcg ggcttgagca gagccaggag
    22441 atgctgctga gcgcccggca gaggctcgcc ccctggcctg gcgcccgtct gtccctctgg
    22501 aatgcagaca cgctggcggc gcacgctcac tcggcggaca ttatctggct taataacgcc
    22561 ctgcatcgtc tgctgccgga agatcccggg ctccttgcga cattacaaca gcttgccgtt
    22621 cccggcgcgc tgctctacgt gatggagttt cgccagttaa cgccgtccgc cctactcagc
    22681 acgctcctgt taaccaatgg gcagccggag gccttgctgc ataacagcgc cgactgggcg
    22741 gcattattta gcgcggccgc cttcaactgt cagcatggcg atgaggtcgc ggggttacaa
    22801 cgcttcctcg tacaatgtcc tgacaggcag gtgcgccgcg atccccgtca acttcaggcc
    22861 gccctcgccg ggcgtctgcc ggggtggatg gtgccgcaac ggatcgtctt cctcgacgcc
    22921 ttaccgctga cggctaacgg gaaaattgac taccaggcgc tgaagcgtcg tcatacccct
    22981 gaagcggaaa acccggccga agcggattta ccccagggcg acattgaaaa acaggttgcc
    23041 gccctctggc agcaactctt atcaactggc aatgtcacca gagaaaccga cttcttccag
    23101 caaggcggcg atagcctgct ggcgacccgt ctgaccgggc aacttcatca ggcaggttat
    23161 gaagcgcaat taagcgacct gtttaatcat ccccggctgg cggattttgc cgccacgctg
    23221 cggaaaaccg acgtcccggt cgaacaacca ttcgtccact cccctgaaga tcgctaccag
    23281 ccctttgcgc ttaccgacgt gcagcaggct tacctggtgg ggcgtcagcc gggctttgcc
    23341 ctgggcggcg tcggctcaca tttctttgtt gaatttgaaa ttgccgatct ggatctcacc
    23401 cggctggaga cggtctggaa ccgattaatc gcccgccacg atatgctacg cgccgtcgtg
    23461 cgtgatggac agcaacaggt gctcgaacag acgcctcgct gggtaatacc cgcacacatc
    23521 ctccatacgc ctgaagaggc gttgcaggtg cgcgaaaaac tggcccatca ggtactcaac
    23581 cccgaagtct ggccggtatt cgatctccag gtcggttacg ttgacgggat gcccgcccgc
    23641 ctgtggctgt gtctggataa cctgttactt gacgggctga gcatgcagat tctgttggcg
    23701 gagctggaac atggctacca atatccgcaa cagttgcctc cgccgctacc cgtcacctac
    23761 agggattacc tgcaacaacc cgcgatccag tcgcttaacg cagattctct ggcatggtgg
    23821 caggcgcaac ttgatgatat tcctccggcg cctgcgttgc cgctgcgctg cttgcctcag
    23881 gaggttgaaa caccgcgctt cgcccgcctg aacggcgcgc tggacagcac gcgctggcat
    23941 cggctgaaaa aacgggcggc tgacgcccat ctcaccccgt cggccgtact gttgtcggtg
    24001 tggtcaacgg ttctctctgc atggagtgca cagcctgagt tcacgcttaa ccttacgctt
    24061 ttcgacaggc gaccgctgca cccgcaaatc aaccagattc tgggcgattt cacctcgctg
    24121 atgctgctga gctggcatcc cggcgaaagc tggctgcaca gcgcgcagtc actacagcag
    24181 cggctgagcc agaacctcaa ccaccgcgat gtgtcagcca tccgcgtgat gcgtcaactg
    24241 gcgcaacggc aaaacgtgcc tgccgttccg atgcccgtcg tctttaccag cgcactgggc
    24301 tttgagcagg ataacttcct cgcccggcgt aatctgctca aaccggtctg gggcatctcc
    24361 cagacgccgc aggtctggct cgatcaccag atttatgaat ccgaaggcga actgcgcttt
    24421 aactgggatt ttgtcgccgc gctgtttcct gccgggcagg tggagcgcca gtttgaacag
    24481 tattgcgcat tgctaaaccg aatggccgag gatgaaagcg gctggcaact gccgctcgcc
    24541 gcgctggtgc ctcccgttaa acacgcaggg caatgcgcag agcgctcacc gcgcgtatgc
    24601 cctgagcact ctcagccaca cattgcggcg gacgagagca ccgtcagcct gatttgcgac
    24661 gccttccgcg aggtggttgg cgagtctgtc acgcccgcag aaaacttctt tgaggcgggc
    24721 gcaacgtcgc tgaatctggt gcaactgcac gttttgttac aacgtcacga attttccacc
    24781 ctgacgttgc ttgacctctt cacccaccct tctcctgctg ccctggccga ttatctggcc
    24841 ggcgtcgcca cggtggagaa aacaaaacga cctcgccctg ttcgccgtcg tcagcggcgg
    24901 atatagcgcg aagcaaactg attttccccg gaacgccatc gcgaacgcat ggcgttccat
    24961 tgactttgtg aatcttagga aacgggaccg attatggata acttgcgctt ctcttctgcg
    25021 ccgacagcag attccattga tgcatcgatc gctcaacact acccggactg cgaacctgtc
    25081 gcggttatcg gctacgcctg ccattttcct gaatcgccgg atggcgaaac gttctggcaa
    25141 aatctgctgg aaggtcgtga atgcagccga cgctttacgc gcgaagagct tctggccgtc
    25201 ggtctggatg ccgccatcat tgacgatcct cattatgtca atatcggtac ggtgttagac
    25261 aacgccgact gcttcgacgc caccctgttt ggctattcgc gacaggaagc ggagtcgatg
    25321 gacccgcagc agcgcctgtt tttgcaggcg gtctggcatg cgctggaaca tgccggttat
    25381 gcccccggcg ccgtccccca taagaccggc gttttcgcct cttcccggat gagtacctac
    25441 cccggtcgcg aagcattgaa cgtgacagaa gtcgcgcagg taaaaggtct gcaatctctg
    25501 atgggcaatg ataaagacta tattgccacc cgcgccgcgt acaaactcaa cctgcacggc
    25561 ccggcgttat cggtacagac cgcctgctcc agctcgctgg ttgccgtgca tctggcctgt
    25621 gaaagcctgc gcgctggcga atccgatatg gcggttgccg gcggcgtggc gctctctttc
    25681 ccccagcagg caggctaccg ctaccagccc ggaatgattt tctctcctga tggtcactgt
    25741 cgtccctttg acgcctcggc tgagggcacc tgggccggta acggtctcgg ctgcgtggtg
    25801 ctgcgtcgcc tgagagacgc gctgctgtca ggcgatccga ttatctcggt gatcctctcc
    25861 agcgcggtca acaacgacgg caacagaaag gtcggctata ccgccccttc cgtcgcaggg
    25921 caacaggcag tcatcgaaga ggcgttaatg ctggcggcca tcgacgacag gcaggtaggt
    25981 tacattgaaa cccacggcac cggcacaccg ctgggcgacg cgattgaaat tgaagcgtta
    26041 cgcaacgtct atgcgcctcg cccgcaggat cagcgctgtg cgctcggttc cgtgaaaagt
    26101 aacatgggcc atctggatac cgcggcgggc attgccggac tgctgaaaac cgttctggca
    26161 gtcagtcgcg ggcaaattcc tcccttactg aattttcaca cccccaaccc ggcgctgaaa
    26221 cttgaagaga gcccctttac cataccggtg tcggcacagg catggcagga cgaaatgcgc
    26281 tatgcgggcg tctcctcctt tggtattggc ggcaccaact gccatatgat cgtcgcctcg
    26341 ctgcccgacg cgctcaacgc gcgcctcccc aatacggata gcggcagaaa aagtaccgcg
    26401 ctgctgctca gcgccgccag cgacagcgcg ttgcggcggc tggcgacgga ttatgccggg
    26461 gcgctgagag agaatgcgga tgccagctct ctggccttca cagccctgca cgcgcgccgt
    26521 ctcgatctcc ccttccgcct ggcggcgcca ttaaaccgtg aaaccgccga ggcgctcagc
    26581 gcctgggcta gtgagaaatc gggggcgctg gtttacagcg gccacggcgc cagcggcaag
    26641 caggtgtggc tgtttaccgg ccagggctcg cactggcgca ctatgggtca aacgatgtac
    26701 cagcactcaa cggcgtttgc cgacacgctg gatcgctgtt tttccgcctg tagcgaaatg
    26761 ctcacgccgt cactgcgcga agcgatgttt aaccccgatt cggcgcagct ggacaatatg
    26821 gcctgggcgc agccggcgat tgtcgcgttt gaaatcgcga tggcggcgca ctggcgtgct
    26881 gaaggactga agccagactt cgccattggg cattccgtcg gtgaatttgc cgctgccgtt
    26941 gtctgcggac actatacgat tgaacaggtc atgccactgg tttgtcggcg cggcgcgcta
    27001 atgcagcagt gcgcaagcgg cgcgatggtg gcggtatttg cagacgaaga cacgctgatg
    27061 ccgctggctc gccagtttga gctggatctc gccgccaaca acggtacgca acatacggta
    27121 ttttccgggc cggaagcccg tctcgcggta ttttgcgcca cgctctcgca gcatgacatt
    27181 aactatcgtc gcctgagcgt aaccggtgcg gcgcactccg ctttactgga gccgatactc
    27241 gatcggttcc aggacgcctg cgcgggactg cacgcggagc cggggcaaat accgattatt
    27301 tccacgctca ccgccgacgt cattgatgag tcaacgctca accaggcgga ttactggcgc
    27361 cgacacatgc gccagccggt gcgttttatc cagagtattc aggtggcgca tcagctcggc
    27421 acccgcgttt ttctggagat ggggcccgat gcccagttgg ttgcttgcgg gcagcgcgaa
    27481 taccgcgata acgcatactg gatagccagc gcccggcgta acaaagaggc gagcgatgtc
    27541 ctcaatcagg ccctgctcca gctttacgct gccggcgtcg ccctaccgtg ggccgacctg
    27601 ctggcgggcg atggacaacg tatcgctgcg ccatgttatc cgtttgatac tgagcgttac
    27661 tggaaagagc gcgtctcccc ggcctgcgag cctgccgacg cagcgctgtc tgccgggctg
    27721 gaggtggcga gtcgcgccgc gacagcgctc gatctccctc gcctggaagc gcttaaacag
    27781 tgcgccacgc gactgcacgc catctacgtc gatcaactgg tacaacgctg taccggcgat
    27841 gccattgaga acggcgtgga cgccatgacc atcatgcgcc gtggacgtct gctgccccgc
    27901 taccagcagc tactccagcg cctgctgaat aactgcgtgg tcgacggcga ttaccgctgc
    27961 accgacgggc gatacgtccg cgcccgcccc attgaacatc aacagcggga atcactgctg
    28021 acggaacttg ccggttattg tgaaggtttt caggctattc ccgacaccat cgcccgtgcc
    28081 ggcgatcggt tatatgaaat gatgagcggc gcggaagaac cggtggcgat tatcttcccg
    28141 caaagcgcct ccgacggcgt ggaagtgctg tatcaggaat tcagctttgg ccgctatttc
    28201 aaccaaatcg ccgccggggt attacgcggc attgtccaga cgcgtcagcc ccgccagccg
    28261 ttgcgtattc ttgaagttgg cggcggaacc ggcggcacca ccgcgtggct gctgccggaa
    28321 ctcaacggcg ttccggcact ggagtaccat ttcaccgata tctcggcgct gttcacccgt
    28381 cgcgcccagc agaaattcgc cgactatgat tttgtgaagt atagcgagct ggatctcgaa
    28441 aaagaggcgc agtctcaggg tttccaggca cagtcttacg atcttatcgt ggcagcgaac
    28501 gtgattcacg ccacccgcca tattggccgc acgctcgata atctgcgccc cctgctcaag
    28561 ccgggcgggc gcctgctgat gcgcgaaatc acccagccaa tgcgtctgtt tgacttcgtt
    28621 ttcggcccgc tggttcttcc gctacaggat ctcgacgccc gcgaaggtga gttattcctc
    28681 accaccgctc agtggcaaca acagtgccgc cacgccggat tcagcaaagt ggcgtggcta
    28741 ccgcaggatg gcagcccgac cgccgggatg agcgaacata tcattctcgc cacgctgccc
    28801 ggtcaggcgg ttagcgccgt aacattcacc gcgccatcag aacccgtgtt ggggcaggcg
    28861 ctgacggata acggtgatta tctcgccgac tggtctgatt gcgcaggtca gcccgaacgg
    28921 tttaacgccc gctggcagga ggcctggcgt ctgctttcac agcgtcatgg cgacgctctg
    28981 cctgtggaac cgccccccgt cgccgccccg gagtggctag ggaaggttcg cttaagctgg
    29041 caaaacgaag ccttttcccg cggtcagatg cgcgttgaag cccgtcatcc tgctggccag
    29101 tggctgccgc tatcgcccgc cgagcctctt cctgcgccgc aaacgcatta tcaatggcgc
    29161 tggacgcccc tcaacgtcgc cagcattgac catccgctta cctttagctt cagcgccggt
    29221 acgcttgcgc gcagcgacga gctggcgcaa tacggcatca ttcacgatcc gcacgcctct
    29281 tcacgactga tgattgttga ggagagcgag gatacgctgg ccttagcgga gaaagtgata
    29341 gcagcgctca ccgccagcgc agccggattg attgtggtta ctcgccgcgc gtggcgagtc
    29401 gaggaaaatg aagcactctc tgcatcccat cacgcgctat gggccttgct tcgcgtcgcg
    29461 gccaacgaac agccggaacg gttgcttgcc gccatcgatc tcgccgaaaa caccccgtgg
    29521 gaaacgctgc atcaagggtt gagcgcagtc tcactatcac agcgctggct cgccgcacgg
    29581 ggtgacaccc tttggcttcc ttcactgtcg cccaatacgg gatgcgccgc tgaattaccg
    29641 gcaaacgtgt ttaccggcga tagccgctgg catctggtga ccggagcgtt tggcggatta
    29701 ggccgccttg ccgtgaactg gctcagagaa aaaggggcgc gacgcatcgc cctgctggca
    29761 ccgcgcgtgg atgagtcatg gctacgcgac gtggagggcg ggcagacgcg cgtctgccgt
    29821 tgtgatgtgg gcgatgccgg gcaactggcc acggttcttg acgatctggc ggccaacggc
    29881 ggcattgccg gagcgattca tgccgctggc gtattggctg acgcgccctt gcaggagctt
    29941 gatgaccacc agctggctgc cgttttcgcg gtaaaagcgc aggcggcaag ccaactgttg
    30001 caaaccctgc gcaaccacga cggacgctat cttattctct actcttccgc tgccgccacc
    30061 ctcggcgcgc cgggtcagag cgcccatgcg ctggcctgcg gctacctgga cgggctggcc
    30121 cagcagtttt ccacccttga tgcgccgaaa acgctctctg tcgcctgggg cgcatgggga
    30181 gaaagcggtc gggcggccac gccggaaatg ctggcgacgc tcgccagccg aggtatgggc
    30241 gcgttaagcg atgccgaagg ctgctggcac ctggaacagg cggtgatgcg cggcgccccg
    30301 tggcgactgg cgatgcgcgt ttttaccgac aaaatgcccc cgttacaaca ggctctgttt
    30361 aacatcagcg ccacagaaaa agccgcaacg ccggtcattc ctcctgctga tgacaacgcc
    30421 tttaacggca gcctgagcga tgaaacagcg gtgatggcat ggctgaaaaa gcggattgcg
    30481 gttcagctaa ggctgagcga tccggcgtca ctgcatccaa accaggatct gttgcaactc
    30541 ggcatggact cgctgctctt ccttgaactc agtagcgata ttcagcacta cctgggtgta
    30601 cgcatcaatg cggaacgggc gtggcaggat ctgtctcctc atggactcac gcagcttatc
    30661 tgttctaagc cagaggcgac gcctgccgct tcgcagccgg aagtgttgcg gcacgacgcc
    30721 gacgagcgtt atgcgccctt ccctttgacg cccattcagc acgcctactg gctggggcga
    30781 acccacctca ttggctatgg cggcgtcgcc tgtcacgtcc tgtttgagtg ggataaacgc
    30841 cacgatgagt tcgatctcgc catactggag aaagcatgga accagctcat cgcacgccac
    30901 gatatgttgc gtatggtggt tgatgccgac gggcagcagc gaatcctggc gacaacgccg
    30961 gagtatcaca tcccgcgtga cgatctgcgc gcgctttccc cggaagaaca gcgcatcgcg
    31021 ctggaaaaac ggcggcatga actgagctat cgcgttttgc ctgccgacca gtggcctctt
    31081 tttgagctgg tggtcagcga aatcgacgat tgccattacc gtctgcatat gaacctcgac
    31141 cttttgcagt ttgatgtgca gagttttaaa gtcatgatgg acgacctggc gcaggtctgg
    31201 cgcggtgaaa cgctggcacc gctcgctatt accttccgtg attatgtgat ggctgaacag
    31261 gcgcgccgac agacatcggc atggcacgat gcctgggatt actggcagga aaaactgccg
    31321 caactgccct tagcgccaga gctgccggtg gttgagacgc ccccggaaac gccacacttc
    31381 accaccttca aatcgacgat cggcaagaca gaatggcagg ccgtgaaaca gcgctggcag
    31441 cagcaaggcg tcacaccgtc tgccgcgctg ctcacgctgt ttgccgccac ccttgagcgc
    31501 tggagccgta ccacaacatt tacgctgaac ctgacgttct tcaatcgcca gccgatccat
    31561 ccgcaaatca accagttgat tggtgatttt acctccgtca cgctggttga ttttaacttc
    31621 tcagcgccgg tgacgttgca agagcagatg caacagaccc aacagcgcct ctggcaaaac
    31681 atggcgcaca gtgaaatgaa cggtgttgag gtgatccgtg agctggggcg cctgcgcgga
    31741 tcacaacgtc aaccgctgat gccggtagtg tttaccagta tgctggggat gacgctggaa
    31801 ggcatgacta tcgatcaggc gatgagccat ctgttcggcg aaccctgcta tgtattcacg
    31861 caaacgccgc aggtctggct ggatcatcag gtcatggaga gcgacggcga gttgatgttt
    31921 agctggtact gcatggacaa cgtgctggaa cccggcgctg ccgaggcgat gtttaatgac
    31981 tattgcgcca tcctgcaagc cgtcatcgcc gcccctgaaa gcctgaagac tctcgccagc
    32041 ggcatcgccg ggcacattcc ccgccgacgc tggccgctga acgcgcaggc ggactacgac
    32101 ctgcgggata ttgagcaggc gacgctcgaa taccccggca tccggcaggc cagagcggaa
    32161 ataaccgaac agggcgcgtt gacgctggat atcgtgatgg ccgacgatcc gtcgccatca
    32221 gcggcgatgc ctgatgagca cgaacttacc caactggcgc tgccgttgcc tgagcaggcg
    32281 cagcttgatg agctggaggc gacctggcgc tggctggagg cgcgagcgct acaggggatc
    32341 gcggctacgc taaatcgtca cggcctgttt accacgccgg agatcgccca tcgctttagc
    32401 gcaatagtac agacgctgtc cgcgcaagcg tctcaccagc gtctgctgcg ccagtggcta
    32461 cagtgtctga cggaaagaga gtggttaatc cgcgaaggtg aaagctggcg ctgccgcatt
    32521 ccgctcagcg agattcctga gcctcaggaa gcgtgcccgc aaagccaatg gagccaggcg
    32581 ctggcgcagt atctggaaac ctgcatcgcc cggcacgacg ccctcttctc cgggcagtgt
    32641 tctccgctgg agttgctgtt caacgagcag catcgcgtta ccgacgcgct gtatcgcgac
    32701 aaccccgcca gcgcctgtct gaatcgctat accgcgcaga ttgccgcctt gtgcagcgca
    32761 gaacggattc tggaggttgg cgccggaacc gcagccacta ccgcgccggt gctgaaggcc
    32821 acgcggaaca cgcggcagtc gtaccacttc acggacgtct ccgcgcagtt cctcaatgac
    32881 gccagagccc gtttccatga tgaatcgcag gtgtcttatg ccttgttcga catcaaccag
    32941 ccgctggatt tcaccgccca cccggaggcg ggttacgacc tgatcgttgc cgtcaatgtg
    33001 ctccacgacg ccagccatgt cgtccagacg ttgcgcagat taaaactgtt gctgaaagcc
    33061 ggcggacgtt tgctgatcgt tgaagcgacg gagcgaaaca gcgtattcca gctggcgagc
    33121 gtgggcttta ttgagggatt aagcggatac cgcgatttcc gccgccggga tgagaaaccg
    33181 atgctcaccc gctccgcatg gcaggaggtt ctcgttcagg ccgggtttgc aaacgagctg
    33241 gcgtggcccg cgcaggaatc gtcgccgctg cgccagcatc tgctggtagc gcgttcgcct
    33301 ggcgtaaatc gcccggataa aaaagccgtg agccgctatt tacagcagcg ctttggcacc
    33361 ggtctgccca ttttacagat ccggcaaaga gaagcgttat ttacgccgct gcatgccccg
    33421 tctgatgcgc cgactgagcc agccaaaccc acgccagttg ccggggggaa tccggcgctg
    33481 gaaaaacagg tggctgaact ctggcaatcg ctgctgtctc gccccgtggc aaggcatcac
    33541 gactttttcg aactgggcgg cgacagcctg atggcgacaa ggatggtcgc gcagctaaac
    33601 cggagaggga ttgccagggc taaccttcag gatctgttca gccattcgac gctgagcgac
    33661 ttctgcgccc atctacaggc ggctacgtca ggagaggaca acccgatacc cctttgccag
    33721 ggcgacggtg aggaaaccct gtttgtcttc cacgcttcag acggcgatat cagcgcctgg
    33781 ctgccgctcg ctagcgcgtt gaacaggcgc gttttcggcc tgcaagcaaa atcgccgcag
    33841 cgctttgcca cgctcgacca gatgatcgat gagtatgtcg ggtgcatccg tcgtcagcag
    33901 cctcacggcc cttatgtgct ggcgggttgg tcgtatggcg cgtttcttgc ggcgggcgcc
    33961 gcacaacgcc tgtacgccaa aggcgagcag gttcggatgg tgttaatcga tcccgtgtgc
    34021 cgacaggatt tctgttgcga aaaccgggcg gccctgctgc gcctgttagc cgaaggacaa
    34081 acgcctctgg cactgcccga acatttcgac cagcagatgc ccgacagcca gcttgccgac
    34141 tttatcagcc tcgctaaaac ggccggtatg gtgtcgcaaa acctgacgct gcaagcggca
    34201 gaaacgtggc tcgacaacat cgcgcatctg ctgcgtttac tgactgagca tacgccgggc
    34261 gaaagcgttc cggtcccctg tctcatggtg tatgccgccg ggagacccgc gcactggacg
    34321 ccagcagaaa ccgagtggca gggctggata aacaacgccg acgacgctgt gattgaagcc
    34381 agccactggc aaatcatgat ggaagccccc cacgttcagg cttgtgcgca acacattacg
    34441 cgctggcttt gcgcaacctc aacgcaaccg gagaacacgt tatgatgccg tccgcctccc
    34501 caaaacaacg cgtactgatt gtgggcgcca aatttggcga aatgtacctg aatgccttta
    34561 tgcagccccc ggaggggctg gaactggtcg gcctgctggc gcagggaagc gcccgttcaa
    34621 gagagctggc tcatgcgttt ggcattccgc tgtatacctc gccggaacag ataaccagga
    34681 tgccggatat cgcctgtatt gtcgtgcgtt cgaccgtcgc cggaggaacc ggtacgcagc
    34741 ttgccagaca ctttctgacg cgcggcgtac acgtcattca ggagcatccc ctccacccgg
    34801 atgacatcag ttccttacaa acgctggcgc aggaacaggg ctgctgctat tgggtaaaca
    34861 cgttttatcc gcatacgcgc gcgggacgca catggcttcg cgatgcgcag cagctgcgtc
    34921 gctgtctggc taagacgccg caggtggtcc acgccaccac cagccgacag ttgctctact
    34981 ccactctgga tttgttgttg ctggctctgg gcgttgatgc cgccgccgtg gagtacgacg
    35041 tggttggcag ctttagcgat tttcactgtc tgagactctt ctggccggag ggagaagcct
    35101 gcctgctgct tcagcgctat ctcgatcctg acgatccaga tatgcatagc cttatcatgc
    35161 accgcctgct gctgggctgg ccggaaggtc atctttcgct ggaggcgagc tacggccccg
    35221 tcatctggtc atccagcctg tttgtcgcag accatcagga gaacgcccac agcctctacc
    35281 gcagaccgga gatcctgcgc gatctgccgg gtctgacgcg cagcgcggcg cccctgagct
    35341 ggcgcgactg ctgcgaaacc gccgggccgg agggcgtcag ctggttgcta caccagttgc
    35401 gcagccatct ggcgggcgaa catccaccag cggcctgcca aagcgtacat cagatcgcgt
    35461 tatcacgttt atggcagcag attttacgta agaccggcaa tgcggagatc agacgcctga
    35521 cgccgccgca ccacgaccgg ttagccggtt tctacaacga tgatgataag gaggcgctgt
    35581 gacgcaatct gcaatgtgca tcccgctgtg gcccgcccgg aacggcaata ctgcgcatct
    35641 ggtcatgtgc cctttcgctg gcggcagcag tagcgcgttt cgccactggc aagctgagca
    35701 actggctgat tgcgcgcttt ctctggtgac ctggccgggg cgcgatcgcc ttcgccatct
    35761 ggaaccgctc agaagcatta cacaactggc ggcactgctg gcgaacgagc tggaagcatc
    35821 cgtatcgcct gacacgccgc ttttactcgc cgggcacagc atgggggcgc aggtggcgtt
    35881 tgaaacctgc cgacttctgg agcaacgggg gcttgcgcca caaggactga ttatttccgg
    35941 gtgccatgcc ccgcatctgc attctgaacg ccagctcagc catcgcgatg atgccgactt
    36001 tatcgctgag ctgatagaca ttggcggatg ttctcctgaa ctgcgggaaa accaggaatt
    36061 aatgtcgctg tttcttcctc ttctgcgcgc tgatttttac gccaccgaga gctatcacta
    36121 cgactcgccc gacgtctgtc cgccgctgcg cacgcctgcg ctgttattgt gcggcagcca
    36181 cgatcgcgaa gcctcctggc agcaggtcga tgcctggcgt cagtggctga gccacgttac
    36241 aggcccggtg gtgattgacg gcgatcattt ctatcccatt caacaagccc ggtccttttt
    36301 tacgcagatt gtccgccatt ttccccacgc attttctgca atgaccgcgt ggcaaaaaca
    36361 gcccagtact tcagaaaggt gacgcatgaa ttcttccttt gaatctctga ttgaacagta
    36421 tcccttaccc attgccgaac agttgcgcca ctgggcggcc cgttatgcct cgcgaattgc
    36481 cgtcgttgat gcaaaggggt cgttaaccta cagcgcgctt gatgcacaag ttgacgaact
    36541 tgccgcaggt ctgtcatcac tgggtttgcg ttcgggggag catgtaattg tgcagcttcc
    36601 caacgacaac gcgtttgtta ccctgctgtt cgccttgtta agactgggcg ttatccccgt
    36661 gctggcgatg ccctcgcaac gggcgctgga tatcgacgcg ctgattgagc tggcgcaacc
    36721 cgtcgcttac gttattcacg gggaaaacca cgcagagctg gcccgacaga tggcgcacaa
    36781 acacgcctgc ttgcgtcatg ttctggtcgc tggagagacc gtgagcgacg attttacgcc
    36841 gctcttctcc cttcacggtg agcgacaggc atggccgcag cctgatgttt ccgccaccgc
    36901 gttgttgttg ctctcaggcg gcacaaccgg cacgcccaaa ctcatcccgc gccgacatgc
    36961 cgactatagc tataacttca gcgcttctgc tgaactgtgc ggcatcagcc aacagagcgt
    37021 gtatctcgcc gtcctcccgg tggcgcataa ctttccgctg gcctgccccg gtattctggg
    37081 aacgcttgcc tgcggcggaa aagtggtgct gaccgacagc gccagctgtg atgaggtgat
    37141 gcctttaatc gcgcaggaaa gagtgactca cgtcgccctg gttccggcgc tggcgcaatt
    37201 atgggtgcag gccagggagt gggaagacag cgacctttcg tcgctgcgcg tcattcaggc
    37261 aggcggcgcc cggctcgacc cgacgcttgc tgagcaggtt atcgccacct ttgactgtac
    37321 cctgcaacag gttttcggta tggcggaagg cctgctctgt tttacccggc tggacgatcc
    37381 gcatgccacc attctccaca gccaggggcg cccgttgtcc cctctggatg aaatccgcat
    37441 cgttgatcaa gacgagaacg acgtcgcgcc gggcgaaacc gggcaattgt taacgcgcgg
    37501 cccttatacc atttcgggct attaccgcgc ccctgcccac aatgcgcagg cctttaccgc
    37561 gcaagggttt taccgcacag gcgacaatgt caggctggat gaggtgggga acctgcacgt
    37621 tgagggacgc ataaaagagc agatcaaccg cgccggagaa aaaatagccg cggctgaagt
    37681 ggaatcggca ctgctgcgtt tagcggaagt gcaagattgc gcggtggtcg ccgcgccgga
    37741 cacgctgctt ggcgagcgga tttgcgcgtt tatcatcgcg cagcaggtgc caactgacta
    37801 tcagcagttg cgtcaacaac tgacccgtat ggggctcagc gcgtggaaaa ttcctgacca
    37861 aatcgagttt ctggaccact ggccgctcac cgccgtcggc aagatagaca aaaaacgcct
    37921 gacggctctc gccgtcgacc gttatcgcca ttctgcccaa taagcgcaaa ccgacccgaa
    37981 acaggttgaa ataaacccgt ttcgggtagc accactatta gaaatagtta tcattttcaa
    38041 ttcaccattg tcggtatttt tggcgtttcg ccgtcttaca gggactcaca acaatgaaaa
    38101 tgacacggct ttatcctctg gccttggggg gattattgct ccccgccatt gctaatgccc
    38161 agacttcaca gcaagacgaa agcacgctgg tggttaccgc cagtaaacaa tcttcccgct
    38221 cggcatcagc caacaacgtc tcatctactg ttgtcagcgc gccggaatta agcgacgccg
    38281 gcgtcaccgc cagcgacaaa ctccccagag tcttgcccgg gctcaatatt gaaaatagcg
    38341 gcaacatgct tttttcgacg atctcgctac gcggcgtctc ttcagcgcag gacttctata
    38401 accccgccgt caccctgtat gtcgatggcg tccctcagct ttccaccaac accatccagg
    38461 cgcttaccga tgtgcaaagc gtggagttgc tgcgaggccc acagggaacg ttatatggca
    38521 aaagcgctca gggcgggatc atcaacatcg tcacccagca gccggacagc acgccgcgcg
    38581 gctatattga aggcggcgtc agtagccgcg acagttatcg aagtaagttc aacctgagcg
    38641 gccccattca ggatggcctg ctgtacggca gcgtcaccct gttacgccag gttgatgacg
    38701 gcgacatgat taaccccgcg acgggaagcg atgacttagg cggcacccgc gccagcatag
    38761 ggaatgtgaa actgcgtctg gcgccggacg atcagccctg ggaaatgggc tttgccgcct
    38821 cacgcgaatg cacccgcgcc acccaggatg cctatgtggg atggaatgat attaagggcc
    38881 gtaagctgtc gatcagcgat ggttcaccag acccgtacat gcggcgctgc actgacagcc
    38941 agaccctgag tgggaaatac accaccgatg actgggtttt caacctgatc agcgcctggc
    39001 agcagcagca ttattcgcgc accttccctt ccggttcgtt aatcgtcaat atgcctcagc
    39061 gctggaatca ggatgtgcag gagctgcgcg ccgcaaccct gggcgatgcg cgtaccgttg
    39121 atatggtgtt tgggctgtac cggcagaaca cccgcgagaa gttaaattca gcctacgaca
    39181 tgccgacaat gccttattta agcagtaccg gctataccac cgctgaaacg ctggccgcat
    39241 acagtgacct gacctggcat ttaaccgatc gttttgatat cggcggcggc gtgcgcttct
    39301 cgcatgataa atccagtaca caatatcacg gcagcatgct cggcaacccg tttggcgacc
    39361 agggtaagag caatgacgat caggtgctcg ggcagctatc cgcaggctat atgctgaccg
    39421 atgactggag agtgtatacc cgtgtagccc agggatataa accttccggg tacaacatcg
    39481 tgcctactgc gggtcttgat gccaaaccgt tcgtcgccga gaaatccatc aactatgaac
    39541 ttggcacccg ctacgaaacc gctgacgtca cgctgcaagc cgcgacgttt tatacccaca
    39601 ccaaagacat gcagctttac tctggcccgg tcgggatgca gacattaagc aatgcgggta
    39661 aagccgacgc caccggcgtt gagcttgaag cgaagtggcg gtttgcgcca ggctggtcat
    39721 gggatatcaa tggcaacgtg atccgttccg aattcaccaa tgacagtgag ttgtatcacg
    39781 gtaaccgggt gccgttcgta ccacgttatg gcgcgggaag cagcgtgaac ggcgtgattg
    39841 atacgcgcta tggcgcactg atgccccgac tggcggttaa tctggtcggg ccgcattatt
    39901 tcgatggcga caaccagttg cggcaaggca cctatgccac cctggacagc agcctgggct
    39961 ggcaggcgac tgaacggatg aacatttccg tctatgtcga taacctgttc gaccgtcgtt
    40021 accgtaccta tggctacatg aacggcagca gcgccgtcgc gcaggtcaat atgggtcgca
    40081 ccgtcggtat caatacgcga attgatttct tctgattatt gtaaaaggga taccgaaaag
    40141 gtatcccttt tacaccacta gttaaaacca gtaactcagc aaagtcgcaa aaaatattaa
    40201 tccatagtga ttatttaaac aatgaaattg cgattaggat aaat
//
LOCUS       DAZFRE010000034        40239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFRE010000034 DAZFRE010000000
VERSION     DAZFRE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.034"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            240..353
                     /gene="yhiY"
                     /locus_tag="ACHKUI_002127"
     CDS             240..353
                     /gene="yhiY"
                     /locus_tag="ACHKUI_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6598315.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            416..1483
                     /gene="yhiI"
                     /locus_tag="ACHKUI_002128"
     CDS             416..1483
                     /gene="yhiI"
                     /locus_tag="ACHKUI_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6598316.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWLLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1480..4215
                     /gene="rbbA"
                     /locus_tag="ACHKUI_002129"
     CDS             1480..4215
                     /gene="rbbA"
                     /locus_tag="ACHKUI_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6598317.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4215..5339
                     /gene="yhhJ"
                     /locus_tag="ACHKUI_002130"
     CDS             4215..5339
                     /gene="yhhJ"
                     /locus_tag="ACHKUI_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598318.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5348..5749)
                     /gene="nikR"
                     /locus_tag="ACHKUI_002131"
     CDS             complement(5348..5749)
                     /gene="nikR"
                     /locus_tag="ACHKUI_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6598319.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(5755..6561)
                     /gene="nikE"
                     /locus_tag="ACHKUI_002132"
     CDS             complement(5755..6561)
                     /gene="nikE"
                     /locus_tag="ACHKUI_002132"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6598320.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(6558..7322)
                     /gene="nikD"
                     /locus_tag="ACHKUI_002133"
     CDS             complement(6558..7322)
                     /gene="nikD"
                     /locus_tag="ACHKUI_002133"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6598321.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(7322..8155)
                     /gene="nikC"
                     /locus_tag="ACHKUI_002134"
     CDS             complement(7322..8155)
                     /gene="nikC"
                     /locus_tag="ACHKUI_002134"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6598322.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPVQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(8152..9096)
                     /gene="nikB"
                     /locus_tag="ACHKUI_002135"
     CDS             complement(8152..9096)
                     /gene="nikB"
                     /locus_tag="ACHKUI_002135"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6598323.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9096..10670)
                     /gene="nikA"
                     /locus_tag="ACHKUI_002136"
     CDS             complement(9096..10670)
                     /gene="nikA"
                     /locus_tag="ACHKUI_002136"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6598324.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(10781..11368)
                     /gene="acpT"
                     /locus_tag="ACHKUI_002137"
     CDS             complement(10781..11368)
                     /gene="acpT"
                     /locus_tag="ACHKUI_002137"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6598325.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYQSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11423..12472)
                     /gene="yhhT"
                     /locus_tag="ACHKUI_002138"
     CDS             complement(11423..12472)
                     /gene="yhhT"
                     /locus_tag="ACHKUI_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6598326.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12571..13821
                     /gene="yhhS"
                     /locus_tag="ACHKUI_002139"
     CDS             12571..13821
                     /gene="yhhS"
                     /locus_tag="ACHKUI_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6598327.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(13825..14382)
                     /gene="dcrB"
                     /locus_tag="ACHKUI_002140"
     CDS             complement(13825..14382)
                     /gene="dcrB"
                     /locus_tag="ACHKUI_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6598328.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(14455..15120)
                     /gene="yhhQ"
                     /locus_tag="ACHKUI_002141"
     CDS             complement(14455..15120)
                     /gene="yhhQ"
                     /locus_tag="ACHKUI_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6598329.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15341..15586
                     /gene="tusA"
                     /locus_tag="ACHKUI_002142"
     CDS             15341..15586
                     /gene="tusA"
                     /locus_tag="ACHKUI_002142"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6598330.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15855..18053)
                     /gene="zntA"
                     /locus_tag="ACHKUI_002143"
     CDS             complement(15855..18053)
                     /gene="zntA"
                     /locus_tag="ACHKUI_002143"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6598331.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(18127..18753)
                     /gene="yhhN"
                     /locus_tag="ACHKUI_002144"
     CDS             complement(18127..18753)
                     /gene="yhhN"
                     /locus_tag="ACHKUI_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6598332.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            18894..19253
                     /gene="yhhM"
                     /locus_tag="ACHKUI_002145"
     CDS             18894..19253
                     /gene="yhhM"
                     /locus_tag="ACHKUI_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6598333.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19256..19525)
                     /gene="yhhL"
                     /locus_tag="ACHKUI_002146"
     CDS             complement(19256..19525)
                     /gene="yhhL"
                     /locus_tag="ACHKUI_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6598334.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19515..20111)
                     /gene="rsmD"
                     /locus_tag="ACHKUI_002147"
     CDS             complement(19515..20111)
                     /gene="rsmD"
                     /locus_tag="ACHKUI_002147"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6598335.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            20261..21757
                     /gene="ftsY"
                     /locus_tag="ACHKUI_002148"
     CDS             20261..21757
                     /gene="ftsY"
                     /locus_tag="ACHKUI_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6598336.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     ASEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            21760..22428
                     /gene="ftsE"
                     /locus_tag="ACHKUI_002149"
     CDS             21760..22428
                     /gene="ftsE"
                     /locus_tag="ACHKUI_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6598337.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLIGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22421..23479
                     /gene="ftsX"
                     /locus_tag="ACHKUI_002150"
     CDS             22421..23479
                     /gene="ftsX"
                     /locus_tag="ACHKUI_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6598338.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23724..24578
                     /gene="rpoH"
                     /locus_tag="ACHKUI_002151"
     CDS             23724..24578
                     /gene="rpoH"
                     /locus_tag="ACHKUI_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6598339.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24850..25953
                     /gene="livJ"
                     /locus_tag="ACHKUI_002152"
     CDS             24850..25953
                     /gene="livJ"
                     /locus_tag="ACHKUI_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6598340.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPESVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(26141..26524)
                     /gene="panM"
                     /locus_tag="ACHKUI_002153"
     CDS             complement(26141..26524)
                     /gene="panM"
                     /locus_tag="ACHKUI_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6598341.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            26948..28057
                     /gene="livK"
                     /locus_tag="ACHKUI_002154"
     CDS             26948..28057
                     /gene="livK"
                     /locus_tag="ACHKUI_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6598342.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28105..29031
                     /gene="livH"
                     /locus_tag="ACHKUI_002155"
     CDS             28105..29031
                     /gene="livH"
                     /locus_tag="ACHKUI_002155"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6598343.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMISSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            29028..30305
                     /gene="livM"
                     /locus_tag="ACHKUI_002156"
     CDS             29028..30305
                     /gene="livM"
                     /locus_tag="ACHKUI_002156"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6598344.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            30302..31069
                     /gene="livG"
                     /locus_tag="ACHKUI_002157"
     CDS             30302..31069
                     /gene="livG"
                     /locus_tag="ACHKUI_002157"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6598345.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            31071..31784
                     /gene="livF"
                     /locus_tag="ACHKUI_002158"
     CDS             31071..31784
                     /gene="livF"
                     /locus_tag="ACHKUI_002158"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6598346.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            32181..33497
                     /gene="ugpB"
                     /locus_tag="ACHKUI_002159"
     CDS             32181..33497
                     /gene="ugpB"
                     /locus_tag="ACHKUI_002159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6598347.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            33595..34482
                     /gene="ugpA"
                     /locus_tag="ACHKUI_002160"
     CDS             33595..34482
                     /gene="ugpA"
                     /locus_tag="ACHKUI_002160"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6598348.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            34479..35324
                     /gene="ugpE"
                     /locus_tag="ACHKUI_002161"
     CDS             34479..35324
                     /gene="ugpE"
                     /locus_tag="ACHKUI_002161"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6598349.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            35326..36396
                     /gene="ugpC"
                     /locus_tag="ACHKUI_002162"
     CDS             35326..36396
                     /gene="ugpC"
                     /locus_tag="ACHKUI_002162"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6598350.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            36393..37136
                     /gene="ugpQ"
                     /locus_tag="ACHKUI_002163"
     CDS             36393..37136
                     /gene="ugpQ"
                     /locus_tag="ACHKUI_002163"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6598351.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(37123..37563)
                     /gene="yhhA"
                     /locus_tag="ACHKUI_002164"
     CDS             complement(37123..37563)
                     /gene="yhhA"
                     /locus_tag="ACHKUI_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6598352.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            37683..39416
                     /gene="ggt"
                     /locus_tag="ACHKUI_002165"
     CDS             37683..39416
                     /gene="ggt"
                     /locus_tag="ACHKUI_002165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6598353.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPFSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(39454..39738)
                     /gene="yrhB"
                     /locus_tag="ACHKUI_002166"
     CDS             complement(39454..39738)
                     /gene="yrhB"
                     /locus_tag="ACHKUI_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO6598354.1"
                     /translation="MITYHDAFAEANNYLDDANLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTTKPLEEYLQDYEIKKATFGLP"
     gene            complement(39938..40093)
                     /locus_tag="ACHKUI_002167"
                     /pseudo
     CDS             complement(39938..40093)
                     /locus_tag="ACHKUI_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9341 a        10399 c        11257 g         9242 t
ORIGIN      
        1 cgcacatcag gcgataataa cgctatgtga tagtcatcag gtttccctgt tacggaaacc
       61 tgtgcaacag aatctgtgaa cctgtacaga atatttttgg aaaaaacacc agcgaacgga
      121 agaacatcgc gatgtatcac aagttgatgg caatcaaata gacaacatga atataactgt
      181 tagtatttaa ttgatttcac ttatgagtat gcgtgtttca tttacggcca ggaatgctga
      241 tgatgacaac attactaccc gttttcacta agccttcccc attggcgctc aatgctctgt
      301 gcgctggtcg tatttgccat ttccttctta tcccggatgg aagaatccgc tgaacatcgt
      361 tcggtggcag ttgtaacacg cgtcgattgc ttacagggat tatggcggat cgattatgga
      421 taagagtaag cgccatctgg cgtggtgggg tgtcggggca ctggcggtgg ctgccgtcgt
      481 ggcgtggttg ctgttgcgcc cggcgggtgt gccggaaggc tttgctgtca gcaatgggcg
      541 cattgaagcg acggaagtgg atatcgccag caaaattgcc gggcgtatcg acaccattct
      601 ggtgaaagaa ggccagtttg ttcgagaagg tgaagtgctg gcgaagatgg atactcgcgt
      661 gttgcaggaa cagcgactgg aagccatcgc gcaaatcaaa gaagcacaaa gtgccgttgc
      721 cgccgcgcag gctttgctgg agcaacgcca aagcgaaact cgtgccgcac agtcgctggt
      781 taatcaacgc caggcagaac tggactccgt agcaaaacgt catacgcgtt cccgctcact
      841 ggcacaacgg ggggcaattt ctgcgcaaca gttggatgac gaccgcgccg ccgctgagag
      901 cgcccgagct gcgctggaat cggcaaaagc tcaggtttct gcttctaaag ccgctataga
      961 ggcggcacgc accaatatca ttcaggcgca aactcgcgtc gaagcggcac aagccactga
     1021 acggcgcatt gccgcagata tcgatgacag cgaactgaaa gccccgcgtg acggacgcgt
     1081 gcagtatcgg gttgccgagc caggcgaagt gctggcggca ggcggtcggg tgctgaatat
     1141 ggtcgatctc agcgacgtct atatgacctt ctttctgcca accgaacagg cgggcacgct
     1201 gaaactgggc ggtgaagcac gtttgattct tgatgctgca ccagatctgc gtattcccgc
     1261 gaccatcagt tttgtcgcca gcgtcgccca gttcacgcca aaaactgtcg aaaccagcga
     1321 tgagcggctg aaattgatgt tccgtgtcaa agcgcgtatc ccaccggaat tactccagca
     1381 gcatctggaa tatgtcaaaa ccggattgcc gggcgtagcg tgggtgcggg tgaatgaaga
     1441 acttccgtgg cctgacgacc tggtggtgag gttgccgcaa tgacgcatct ggaactggtt
     1501 cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc attatggaaa aaccgttgcg
     1561 ctgaacaata tcacactcga tattccggcc cgttgcatgg tcgggctgat tggaccggat
     1621 ggtgtcggta aatcgagctt gttgtcgttg atttccggtg cccgcgtcat tgagcagggc
     1681 ggcgtgatgg tgctgggcgg cgatatgcgc gacccgaagc atcgccgcga cgtctgcccg
     1741 cgcattgcct ggatgccgca ggggctgggc aaaaatctct accacacctt gtcggtgtat
     1801 gaaaacgtcg attttttcgc ccgcctgttc ggtcacgaca aagcggagcg ggaagtgcgt
     1861 attaatgagc tgctgaccag caccggatta gcaccgtttc gcgatcgtcc ggcagggaaa
     1921 ctctccggcg ggatgaagca aaaacttggg ctgtgctgtg cgttaatcca cgacccggaa
     1981 ttgttgattc ttgatgaacc aacaacgggg gttgacccgc tttcccgcgc ccagttctgg
     2041 gatctgatcg acagcattcg ccagcggcag agcaatatga gcgtgctggt tgccaccgcc
     2101 tatatggaag aggcggaacg cttcgactgg ctggtagcga tgaatgccgg agaagtgctg
     2161 gcaaccggta gcgccgaaga actgcggcag caaacgcaaa gcgccacgct ggaagaagca
     2221 tttataaatc tgttaccgca agcacaacgg caggcgcatc aggcggtagt gatcccaccg
     2281 tatcaaccgg aaaacgcaga gattgccatc gaagcgcgcg atctgaccat gcgttttggt
     2341 tcctttgtcg ccgttgatca cgtcaatttt cgcattccac gcggggagat ttttggtttc
     2401 ctcggttcga acggctgcgg taaatccacc accatgaaaa tgctcaccgg actgctgccc
     2461 gccagcgaag gtgaggcgtg gctgttcggg caaccggttg atccaaaaga tatcgatact
     2521 cgtcgccggg tgggctatat gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg
     2581 caaaacctgg agttacatgc tcgtctgttt catatcccgg aagcggaaat tcccgcgcga
     2641 gtggctgaaa tgagcgagcg ttttaagctc aacgacgtcg aagatgtatt gccggagtca
     2701 ttgccgctcg gcattcgcca gcggctatcg ctggcggtgg cggtgattca tcgcccggag
     2761 atgttaatcc ttgatgaacc gacttctggc gtcgatccgg tggcgaggga tatgttctgg
     2821 cagttgatgg tggatctctc gcgccaggac aaagtgacta tcttcatctc cacccacttt
     2881 atgaacgaag cggaacgttg tgaccgtatc tcactgatgc acgccggaaa agtgctcgcc
     2941 agcggtacac cgccggaact ggttgagaaa cgcggagccg ccagtctgga agaggcattt
     3001 atcgcttatt tgcaggaagc ggcagggcag agcaacgaag ctgaagcgcc gccggtggta
     3061 cacgacacca cccacgcgcc acgtcaggga ttcagcctgc gccgtctgtt tagctacagc
     3121 cgccgcgaag cgctggaact gcgacgcgat ccggtacgtt cgacgctggc gctgatggga
     3181 acggtgatcc tgatgctgat aatgggttac ggcatcagta tggatgtgga aaacctgcgc
     3241 tttgcggtgc ttgaccgcga tcaaaccgtc agtagccagg cgtggacgct caatctctcc
     3301 ggttcccgtt actttatcga acagccaccg ctcaccagtt atgacgagct tgatcgtcgg
     3361 atgcgtgcgg gcgatattac ggtggcaatt gagatccctc ccaatttcgg gcgcgatatc
     3421 gcgcgtggta cgcccgtgga actcggtgtc tggatcgacg gtgcgatgcc gagccgcgcc
     3481 gaaacggtaa aaggctacgt gcaggccatg caccagagct ggctacagga tgtggcgagc
     3541 cgacagtcca cacccgccag ccaaagcggg ctgatgaata ttgagacgcg ctatcgctat
     3601 aacccggacg taaaaagcct gccggcgatt gttccggcgg tgatcccgct tctgctgatg
     3661 atgatcccgt caatgctaag cgcccttagc gtggtgcggg aaaaagagct agggtcgatt
     3721 atcaaccttt acgtgactcc caccacccgt agcgaatttt tacttggcaa acagctgcca
     3781 tacattgcgc tggggatgct gaactttttc cttctctgcg ccctgtcggt gtttgtgttt
     3841 ggcgtaccgc ataaaggcag tttcctgact ctcaccctgg cggcgctgct gtatatcatc
     3901 atcgccaccg gaatggggct gctgatctcc acctttatga aaagccagat tgccgccatt
     3961 ttcggtacgg cgattatcac gttgatcccg gcgacacagt tttccgggat gatcgatccg
     4021 gtagcttcgc tggaaggtcc aggacgttgg atcggcgagg tttacccgac cagtcatttt
     4081 ctgactatcg cccgcgggac gttctcgaaa gcgctggatc tgactgattt gtggcaactt
     4141 tttatcccgt tgctgatagc cataccgctg gtgatgggct taagcatcct gctgctgaaa
     4201 aaacaggagg gatgatgcgc catttacgca atatttttaa tctgggtatc aaagagttgc
     4261 gcagtctgct cggtgataaa gcgatgctga cgctgattgt cttctcgttt acggtgtcgg
     4321 tgtattcgtc agcgaccgtt acgccaggat cgttgaacct cgcgccgatc gccattgccg
     4381 atatggatca atcgcagtta tcgaaccgga tcgttaacag cttctatcgc ccgtggtttt
     4441 tgccaccgga gatgatcacc gccgatgaga tggatgccgg gctggacgcc ggacgttata
     4501 ccttcgcgat aaatattccg cctaattttc agcgtgatgt cctcgccgga cgccagccgg
     4561 atattcaggt gaacgttgat gccacgcgca tgagccaggc atttaccggc aatgggtata
     4621 tccagaatat tatcaacggt gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg
     4681 aaccgttggt atcgctggaa acacggatgc gctttaaccc gaaccttgat cccgcgtggt
     4741 tcggtggggt gatggcgatc atcaacaaca ttaccatgct ggcgattgta ttgaccggat
     4801 cggcgctgat ccgcgagcgt gaacacggca cggtggaaca cttactggtg atgccgataa
     4861 cgccgtttga gatcatgatg gcaaaggtct ggtcgatggg gctggtggtg ctggtggtgt
     4921 cgggattatc gctggtgctg atggtgaaag gcgtgctggg cgtaccgata gaaggctcga
     4981 tcccgctgtt tatgctgggc gtggcgctca gtctgtttgc caccacgtca atcggtattt
     5041 ttatggggac gatagcgcgt tcaatgccgc aactggggct gctggtgatt ctggtgctgc
     5101 tgccgctgca aatgctttcc ggtggttcca cgccgcgcga aagtatgccg cagatggtgc
     5161 aggacattat gctgaccatg ccgacgacac actttgttag cctcgcgcag gccatcctct
     5221 accgtggtgc cggattcgaa atcgtctggc cgcagttcct gacactgatg gcaattggcg
     5281 gcgcgttttt caccatcgca ctgctgcgat tcaggaagac gattgggaca atggcgtgat
     5341 tacggggtta atcttccttc ggcaggcact gcaaatgccc gtgccgcacg ccgcgctggg
     5401 cgatgacgtc atcggcaaaa tgctgcacgt cgcccatgtc acctttcaac acggcgattt
     5461 ccagacagtc gtcgtgatta atgtgcacat gcagagtagc aaccgagaga tcgtggtgat
     5521 gatgctgggt ggagacaata cggctggcta agtcgcgttt ttcgtgttca tacacatacg
     5581 acagcaccgc gaaaccttgt gtgccgtgtt gttgagtggc ttcctgcgcc agggcgctac
     5641 gcagaatatc gcggatcgcc tcggaacggt tgttataacc acgacgctgg ctcaggctgt
     5701 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac
     5761 cttttctgtg gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt gtagcacacg
     5821 tccggcgtca gaggaaaagg ttaatttatc tcccaccacc tgggtttcga cgatttgtcc
     5881 gttgtccatc accatcaccc gctgacagaa gcgttccacc aggcgtaagt cgtgggtgat
     5941 gaacaggcag gcggtgccaa actgttgttg cagctttttc agcaggcgaa tgacgcccgc
     6001 ctgtaacacg agatcaaggt tcgaaaccgc ttcatccaga atcagcagtt tcggttcgac
     6061 cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact gcggtgggcg
     6121 tttgtcgagc acgctgtcgt cgagatcgac cgccttcagc atttcactgg cgcgcgccag
     6181 ttgttcggct tttttcagcg atagcaggtg gcgcatcggt tcacgcagga tctcgcgcac
     6241 ggttttgcgc ggattcacgg cgctgatgga gtcctgaaac accatttgaa tatcacggcg
     6301 gaacgcttta cgttgcgcgc gattgagctt cgccaacggt tcgccgcgcc agctaatatt
     6361 tccctgtgag ggcgactcta accctaccag cagccgcgcg agggtacttt ttccgcagcc
     6421 gctgcgcccc agcagggcga cggtttcacc gcttttcagg gccagggaaa cgttattcag
     6481 caccgcctgg tgttgatgtt tgccgctaaa tccaccgtgc gcgtagtgat gggaaaggtc
     6541 ggagacgtta agtaaagtca tgatgccagc tccataccgt agagggcgag atgagcggaa
     6601 accaggctgc gcgttaccgc atgtttgggg gcgttaaaca gcgtttctac atcgccctgt
     6661 tcaacaatct taccgtgaga cattaccgcc acatcgtctg ccagacgcgc taccacgccc
     6721 atatcatggg taaccagcag cattcccggt gcttgttttt gcataatgct ttccagcaga
     6781 tcgaggatgc gcgcctgtgc taccacgtcg agatcggtgg tcggttcatc ggcgatgata
     6841 aacggtgatt cgcacagcac cgccatcgca atcatcatgc gctgcaacat gccgccgctc
     6901 atctcgaacg ggtagagctt cagcacgcgc gcggcgtttt ccagccccac cgcttctatg
     6961 gcagcggtaa gcgtagcgtc atcggcgggt ttccccagcg ccaggcaggt ttcacgcgcg
     7021 tgggtgtgca tggtgtgcag cggattaaag gcactgcgcg ggttctgcat gatggtggca
     7081 attttgattc cgcgcagggc gcagggagaa accggttttc catcggccaa aatttccccc
     7141 gccgtctggc gaacgccagc aggcagaatg cccagcgtcg cggcgcaggt cagcgacttc
     7201 ccgctgccac taccgccgac taacgccagc acacgcccac gttgcagggt taacgatacg
     7261 ccgtgcacca gcggctgcgc ggcctgtagc gcgatattac ggagttcaat ctgttgtggc
     7321 attagtgtgc gtgctccgtc accagatgag ggtccagatg atcgcgcagt gcgtcaccca
     7381 ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac atttgcaccg
     7441 gctgggtcca gatatactga cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg
     7501 cggtcacgcc gagaccgagg aaggacatcc ccgcgacgtg tagcatcata tgaccgatat
     7561 ccagcgtcgc cagcaccagc agcgagggga tcaccgcacc tgccagatga tcaataaaca
     7621 cccgcacatg gcccgcgcca gaaagccgtg atgccagcac aaactcgcgc tggcgcagtg
     7681 aaatcaccag gctacgcacc atgcgtgcat accacgccca gtgcgacagg gcgatggcta
     7741 taatcacatt ggtcaacccg gtgccgagca cgccgaccat aaagaacgac agaatcgagg
     7801 ttgggaaggt cataaacata tcagcgacgc gcatggtggc ctgatcaacg cgcccgccaa
     7861 tcagcccggc gctgccgcca ataatcagcc ctaatgtcag caccaacagc aggcaggcca
     7921 ttaccgagcc gagcgacacg cgggtcgctg ccatcagccg cgagaaaata tcgcgcccta
     7981 aatgatcggt gcccagccag tgctgcgcat ccggcgagag caggcgcgac ggcaaatcaa
     8041 tagcctgagg gtcatacggc agccaccact ggctggtgag cgcaatcagc gccagcaggg
     8101 cgataatgat cagcgccagg cgaaccgacc agcgggagga gaggaaaaag ttcacgcgtg
     8161 cgctccttca tgacgacgaa tacgcgggtc cagcgcggcg ttgagcaaat cgacaatcaa
     8221 attacagacc acaaaaacca ccaccatcat cagcgtaaag cattggatca ccggatagtc
     8281 acggttaaaa atcgccgaca ccgcatagcg cccgacgccc ggccaggcaa agatgttttc
     8341 gataatcatc gtcccgccaa tcagttcgcc aatgtgcatc cccacggcag tgatcatcgg
     8401 cagcgaggca ttgcgcagaa tatggcggcg ttcggtctgt ttgtcgctca gaccacgcag
     8461 tcgtgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc gcagcaaacg
     8521 cgcgttaatc gccagcgaca taaaggcgat ggataccgca ggcaaaatga tgtgctgcca
     8581 gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca tcaccagtaa
     8641 aaacgccagc cagaagttag gcatcgacac gccgagaaac gcgatgaaac ggacggcgaa
     8701 atccggcaga cgatcgcgat ggcgcgccgc ccagataccg agcggtacgg aggtgagcag
     8761 aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaaat tcagcatatc
     8821 gtccagcacc gggcgttggc tggcgaatga gataccaaag tcaagatgca gcgccttcca
     8881 caaccaggtg ccatactgga cgtacagcgg ctgatccagc cccagcatag ttcgagtaga
     8941 ggccagcatc tccggcgtcg gcggcaggtt agacagacgg agataatcga gcgccgggtc
     9001 accggtgccg aggcgcagca tcaaaaaaat gatcaccgag gcggcgagca ccatcgggat
     9061 cagcagcaga aagcggcgta atacataacg caacattaag gtttcaccgg tttaatctgt
     9121 tcgaacggga tttcggtggc gatcggcgcg taggggatgt tacccagttc cggttttgaa
     9181 accaccatca ttgagatgta actgataggc agataaaccg cctcgtcatg cagacgggtc
     9241 agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag cacttcgccg
     9301 atctctttat caatcagcgg cttgtcggct aaaccttgct gtgcctggaa gtcagcgtgc
     9361 gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc ccaggtgcgg
     9421 tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct ctcttcttcg
     9481 ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat ttccgccatc
     9541 gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg ctgaccattt
     9601 ttctcgcgga tgtctttgcc tgcaggcagc gtccaaccgg ctttttccag caacgctttc
     9661 gctttctgcg gatcgtactg gcgcggtttc aggccgaggt tggcgtaggg cacagaaggg
     9721 gcaaacaggg tgtcggcgac ctgctgggta ccatacaacg cgttatcaat cagcgatttt
     9781 ttgttcaccg cgtaattaag agcttcacgt actgccagct cgttggtggg ggctttggcg
     9841 gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgagtgtg gtaagccggg
     9901 ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc gtacagcagg
     9961 tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat cacgttaaag
    10021 gtgatctttt taatcgccgg cttttcgccc cagtagtttt cgttacggac gaagacatcg
    10081 tactgattca gtttcgattc ctgtaaaacc cacggcccgg tgccaatcgg cgctttaatt
    10141 ccgttcatgg tttcgtggtt tttaaactgc gagggtgcga taaaacggaa aggacggggc
    10201 agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtgatttg cagctcgttt
    10261 ttgctgagtg ctttgacatc aacaatctgg tttgccagct ccagccaggc gtgacgttga
    10321 cggttatcga gcactgcacg gaagttttct gccgccgcct ctgcatcaaa cggttcgccg
    10381 ttggagaact tcacgtcatc acgcagggtg aaggtccagg ttttaccatc ttctgaatga
    10441 gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt caccagcggt
    10501 tcataaacca tgctctgggc gaacatttgg ttaggcgtgt aaaggtgcgg gtttagtggc
    10561 ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg gacgataaaa
    10621 gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tagagaacat ggtaacccca
    10681 atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt catactgatt
    10741 acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc
    10801 attgcacact gtcggcggtg agcgtaaagg gggtaggggt gcagatcgcc aggcttaaat
    10861 tttctagctg acaatggctg actgacagcg aggactgata ggtgctgtct acgctgacga
    10921 tttgccaggc gcttccgcca cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc
    10981 agaacatttc cagttgctga tcaggatgca cggcgtccat ctcggcatgt tccccgaggc
    11041 tgaatacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc
    11101 agccgacttc gccttcatcg ctcaacagca gggcgatatc gtcaccgcta tggcttaagt
    11161 tgaaccatag cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca tagatgatct
    11221 ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgtcc cgccagccag cgttcgcgtc
    11281 gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag aggagctgcg cttaaggtcg
    11341 aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga aaatgaaagt
    11401 gcgtatcgta acacttgtcg cctcatcccg gtaaccgact tttcggtctg cctggcccca
    11461 gtaaaatcgc cagtttgctg ccgcctttgg tggtttccat ccagatttta cacacgctgg
    11521 ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccaa attaacaatg
    11581 aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt ggttctaaaa
    11641 tattgccgat gaccatatgg accactaaaa acaatgcgcc gaccagaata cattcgtaaa
    11701 taccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc gcaccgatat
    11761 tgggcacgta gttgagcaaa aacgccagta ctgcccacat cagcgcaaac tgcaccccca
    11821 tcagctccag ccccagccag acgattatgc ctgtccataa actgagtagc gtctttaacg
    11881 ccagataatg cgaaacgcct ttaagtgcgc ggtgtagtcc ggcgatgtga atctgtggat
    11941 tattcaacgc gaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc ataaaaacta
    12001 cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc attagcgccg
    12061 tggtgaaggt aaccactttt tccgagtcca tccgctgtag tattcgctcc ggcgacatat
    12121 gcaaattaag aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc agctccttat
    12181 taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg ccgaccagcg
    12241 cggtgagtgc gatcagcatc accacaacca caatcgtaat ggcaacgggg cgttgtactc
    12301 cccgacgaat aaaccaggtg acgagcgggt tgagaacgat ggcaaaaaac agcgccagta
    12361 atagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact accagcgagg
    12421 ccaacttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc attggggctt
    12481 ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc tgattcttac
    12541 tgctgatttt cacgtcagca catggtaaaa atgaaacact gttgtaaaaa tgtggtgatc
    12601 ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa tttgcgcatt
    12661 gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc gctcgctgta
    12721 ttaccgggct atgtccatga tgtgatgggc tttagcgctt tctgggcagg attggttatc
    12781 agcctgcaat atttcgccac cttgctgagc cgtcctcatg ccggacgtta cgccgattta
    12841 ctgggaccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag cggtctggga
    12901 tatctgacgg caggattaac cgccagtctg cccgtcatca gcctgttatt actgtgcctg
    12961 gggcgcgtaa tccttgggat tggacaaagt tttgccggaa cgggatcgac cctgtggggt
    13021 gttggcgtgg ttggttcgct acatatcggg cgggtgattt cgtggaacgg tattgtcact
    13081 tacggggcga tggcgatggg tgcgccgtta ggtgtcgtgt tttatcactg gggcggattg
    13141 caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt ggcgatcccg
    13201 cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgtgcggt gcttgggcgc
    13261 gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt tatcgccacc
    13321 tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc gctgacgctg
    13381 tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa ccgtatcggc
    13441 ggcttaaacg tggcgatgat ttgctttagc gttgagataa tcggcctgct actggttggc
    13501 gtggcaacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc ggggttttcg
    13561 ctggtgttcc ctgcattggg cgtagtggcg gtaaaagcgg ttccgcagca aaatcagggg
    13621 gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac cggaccactg
    13681 gctgggctgg tgatgagttg ggcgggcgtt ccggtgattt atctggcggc ggcgggactg
    13741 gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccggt ggaaatacct
    13801 gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga tgttttctgc
    13861 ggtggtctgt gctttttgct gattgtcagc tggcagcgta atttgcatgg ttagcagttg
    13921 attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg cggagataat
    13981 gctgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg ttaccacttg
    14041 cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca ccgccagatc
    14101 ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg cgtcggacca
    14161 gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca tatccgctgg
    14221 cagcgagaaa cttaacttgc catcaagtag gttgacggga ttcccggtcg cgttactttc
    14281 tgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg caagccccat
    14341 aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct tcctttcgat
    14401 aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga acctttaact
    14461 cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca tattgagtag
    14521 aacgccatac attggcagga agaaaacgat actgattaac actttgaaac agtaatcgac
    14581 cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc agaaggcaat
    14641 gaagaagaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg tcggtgccag
    14701 ccaccagtga cgactctgac gcaggcggtt aaaaacgtgc acgtcgagga tctgccccag
    14761 cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga agtgggcgag
    14821 cgcgccgaat ccttgccatg accccatata aaacagcgat gagatgacgt aggagatcag
    14881 caatgcgggg atcattaccg caaagatgat gcgacgagcc agcggcgcgc caaaaatacg
    14941 cacggtcagg tcagtagcga gaaagataaa cggaaagcta aacgcgcccc aggtggtatg
    15001 aaaaccaaaa atggagaccg gaagctgaac cagatagtta ctggaggtga tcaccagaag
    15061 atgaaataac gataaccaga acaacgcctt atagcgttgc gtttgcgaga aaacgttcat
    15121 attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga cacgtctgtt
    15181 accttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac tgctttacgt
    15241 tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac ggactcacag
    15301 ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc tcttttccag
    15361 ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga tgatggtgcg
    15421 caaaaccgtg cgcaatatgc agcctggcga aacgttgctg attatcgccg acgatccggc
    15481 cactacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg ttgctaaaga
    15541 gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg ggaagatggg
    15601 cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga ggggaccgat
    15661 cgctctcaat gttgctgctt gaaaccggtt tgtaggccgg ataaggcgtt caagctgcat
    15721 ctggcgatac ggtgcacaat gcctgatgcg acgctgtcgc gtcttatcat gcctacgagt
    15781 tcgatgctct gtgtaggccg gataaggtgt tcacgccgca tccggcattg ttggaccgta
    15841 gactgatagc ctctttattt cctgcgcaat aaccgtaacg cattcgccgt taccagcacc
    15901 gtcgcacccg tatctgccag caccgccagc cacaggccgg tcatccctaa cagcgtggtg
    15961 acgaggaaaa cgcctttcag ccccagcgca atagtgatgt tctggcgaat attcgcgtga
    16021 gtggcgcgcg ccagttcaat catttgcacc aggccgcgca ggtggttatg cgtcaacgcc
    16081 gcgtcggcgg tttccagtgc gacatctgtg ccactgccca ttgcaatccc aatagcggcg
    16141 gctttcatcg ctggcgcgtc gttaataccg tcgccgacca tcgccagcgg cgcatgttga
    16201 ttcagctcgg tcaccgcttt aactttatct tccggcaaga ggcccgcttt aaattccagc
    16261 cccagttccc cggcgattgc cgctgctgcg cgtggattat cgcctgtaag gatcacccct
    16321 ttgacgccca gcgcgttcag ttcactgata gcagttgcag catcggcgcg cagggtatcc
    16381 tgcaacgcaa tgacacccag cacgtcatca ttacgtacta ccagcactac cgtttgcccg
    16441 gcgctttcca gttcattaat caaaccagca aatgcatcag caggatgttt tcctgcagcg
    16501 catatcaaca cgcgctcacc gttaacctgc gcttcaatgc cagacccgac cagcgcccgc
    16561 tgtgattggg cggtgggaat ggcgagttca gcaacctgtg cttcgcgcac aatggcctgt
    16621 gccagtggat gcgtcgcgcc ttgctcgacc gccgccgcca gtgtcagcag ttcagattca
    16681 ctaatacccg ttgccggatg aatcgcggta acgcgcggtt taccgacggt cagcgtaccg
    16741 gttttatcaa acgccacctg ggtaacacga cccagctgct ccagcgccgc gccgccttta
    16801 atcaacgccc cacgacgcgc tgctgccgcc agcccggagg taatcgccgc gggcgtggag
    16861 ataactaaag cacacgggca accaatcagc agcagcgtca gccctttata aatccactcc
    16921 tgccagctgg cggcaaacag cagcggcggc accagcgtta ccagcagggc gacggccata
    16981 atcgctggcg tgtaaatacg gctgaaacgg tcgataaacc gctcaatagg tgcgcggcgc
    17041 tcttcggctt cttcaatcag tttcagaatc cggtcaatgg cgctggcacc cggttcagac
    17101 agcacttcca gcgttaccag acggtctacg ctggtggcgc ctgcaggaac tttatcgccc
    17161 gtcgcgcgct ccacaggaat ggattctccg gtcagggcac tttcatcaaa actggcaaac
    17221 ggtgagagca gtttaccgtc ggcaggcaaa cgcccacctg ccgcaacttc aatcacatcg
    17281 ccaggccgca ggctgtttat tgccacctct tcacgctcac cgttacgcag acgcgtggcg
    17341 gtttctggct tcagcgccat taacgcgcta acgccctgac gcgcgcggct ggcggcccag
    17401 ccttccagtc gttcaccaat caaaaacagc agcaacacca tcgcagcttc agccgttgcg
    17461 ccaataaaca gcgcaccaat agcggctacg ctcattaagg tttcaatggc gaagtagctg
    17521 ccagatttga tcagccgtaa tgcctgacga gcaattgggt acagcccaac cagcgtggtc
    17581 gcgataaacg ccagttgacc aaacggatga ttgaactgct ccagccccca actgattgcc
    17641 atcatcacga taagcgtaat cagcggcaga ttctctttca ggcgtgattc ttgtggttcg
    17701 tcggaggcct gttcatcgcg cagggaatag cccgcttttt gcaccgcaga ttcaacttgt
    17761 gcccggatgt cattgtcggc atccaccacc agtttttcgg tggcgaacaa cacctgtacc
    17821 tgattcacgc ctgcaagctg gcgcacggca ttttccactt tgcgcgcaca ggcggcgcag
    17881 tccataccgc tgactttcca gctatagcgg gtgccggaga cgttttcaga gagagttggc
    17941 gtgctggaac atgcgccgtc gcagcaacag tcgttggtgt tctgtaccgt agttagcggt
    18001 ttgaacgcag caaattgagg ggctttcttg ccgtgattgt caggagtcga catggcatcc
    18061 tccggttaag ttttttctca ttaaccgaag gatacactct ggagtcgact ccagagtcaa
    18121 gttttatcag agatacagcg agcggacgat gaggaagtga ccagcaaagt agcaggccgc
    18181 ggcaatggcg ttatccgcac ggaagcgtcg gcgatagtga ctacccagcc agacaaagtt
    18241 actgataaac agcaacgaag cgccgacaaa cgcagagagc gccggagcgg tcggacggaa
    18301 gaaccacagt tcacctgcca gccacaccat caccagcgtc atgccgataa acgtgcagat
    18361 aggccaacga tactcttcca gacgcgtcca gataatcgcc agtaacagcg cgcccagaac
    18421 cagcagcacc aacggcagag gccagaagaa agagagcgtc atctgactgg cgaaatagat
    18481 ggtgtacagc aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg
    18541 caacagggtt agcgcatcgc ccagcagtga ggcacacagc cccgccagca ccagatagct
    18601 aatggcgtcg aacatcggcg cttgccaggc cagcaacagc aggagaagaa gggttaacgg
    18661 tttaaacacc cagcgttgcc aggttggccc acgatacgat gcatccacag atagccatgc
    18721 ggaaagacag acagcgataa acgaccaaag catcttagtt ccttgaattc gttatttttt
    18781 cgtaagcgga atgcctacgg tcttgtttcc agtttagtgg cgagggtgga cggatgacaa
    18841 caccataact ggcagggtat gcttatttcc gcattttccg atgagggata aagatgagta
    18901 aaccacctct tttctttatt gttatcattg gcttaatcat cgtcgccgca tcgtttcgtt
    18961 ttatgcaaca acgacgggaa aaagctgata atgatatggc accgctccag caaaagctgg
    19021 tggtggtgag caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag
    19081 tgactccggc aggcaccagt atgcgctatg aggcaagctt caaaccgcaa agcggaggaa
    19141 tggagcagac gtttcgcctc gacgcccagc agtaccacgc cctgacagtg ggtgataaag
    19201 gtacgctgag ctataaagga acgcgctttg tcagctttgt aggcgaacaa taaggttatt
    19261 ttttaacttt aaatttcttc tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc
    19321 gcaccttttc ggcggtggtc atctgcgggc catctttcgg taaagtggat ttcaatagcg
    19381 ccagctgcat accatgcatc agcacggtaa aaatcagcgc cacgttaacg aaaatattca
    19441 gcgggcgtgg gaagggatgc accaggttga ggattaaaaa tccccaaaca cagagcatta
    19501 acaaacgacc aatattaatc agcatcactt tctccttgtg cttcgcgttg atacagtcga
    19561 taagccacct gacccgccac tttttcccga tgtaatgacc agtttgccgg aacagtgggc
    19621 agaccgtttt cgacttcgct ttcgacataa atcagggctt cgtcagccag ccagccgtta
    19681 tcttccagta aatttatcgt ctcttctaac aagccacggc ggaacggtgg atcgacaaac
    19741 acgatattat gcggtgtgcc tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg
    19801 cgtgcattgc ctgcttttag tgtcgccaga ttcttaatta actgctgaga aaccgcacga
    19861 tccatctcaa tcaacgtggc ccccgcagcg tagcgcgata acgcttccag ccccagcgcg
    19921 ccgctcccgg cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc cagccagtta
    19981 aacaacgttt cgcgtacgcg gtcggtggtg gggcgcagac ctggactatc aggaaccggg
    20041 agtttacggc ctcgccactg cccgccaata atgcggattt ggccgctgcc ggaatgattc
    20101 ggttttttca tgataaattg ctcaatccgc cagataacac atacttgcag gcggtgatgt
    20161 gaattaagtt aagtgataga ctatttcatc atttttttag ctgctatgta catagcgtta
    20221 acgctgtgcc atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg
    20281 tggctttttt tcctggctgg gctttggtca aaaagagcag accccggaaa aagagacaga
    20341 agttcagaat gaacaaccag ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc
    20401 ttctgaacac gccgttgaag agcagccgca ggcgcatact gaagccgagg cggaaacttt
    20461 tgctgccgac gttgtggaag ttactgaaca ggttgctgaa agtgaaaaag cgcagcctga
    20521 agcggaagtc gttgcacagc cggaaccggt cgtagaagaa acgccggagc cagtggctat
    20581 cgaacgtgaa gaactgccgt tgccggaaga cgtcaacgcc gaagcggttt cgccagaaga
    20641 gtggcaggcc gaagcggaaa ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa
    20701 agaagaaatc accgacgaag agctggaagc acaggcgctg gcttccgaag cggcagaaga
    20761 ggcggtaatg gtggtttctc cggcagaaga agagcagccg gtggaagaaa tcgctcagga
    20821 gcaggaaaaa ccgaccaaag aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac
    20881 caaagaaaat ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga
    20941 tctgtttgaa gagctggaag aacagctgtt gatcgccgat gtgggcgtgg aaaccacacg
    21001 taaaattatc accaatctga cagaaggcgc atcccgcaag cagcttcgtg atgccgaggc
    21061 gctctatggc ctgctgaaag aagagatggg cgagattctg gcgaaagtcg atgagccgct
    21121 gaatgttgaa ggtaaaacgc cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa
    21181 aaccacgacg attggtaagc tggcacgtca gtttgagcag cagggtaaat cggtgatgct
    21241 ggcggcgggc gatactttcc gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg
    21301 caacaatatt ccggtgattg cccagcatac cggtgcggat tccgcttctg ttatcttcga
    21361 cgccattcag gccgctaaag cgcgtaatat cgacgtcctg attgccgata ccgccggacg
    21421 cctgcagaac aaatcgcacc tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa
    21481 actcgacgtt gaagcgccgc atgaagttat gctgactatt gatgccagca ccgggcagaa
    21541 tgcggtaagc caggccaaac tgttccacga agccgttggc ttaaccggca tcacgctaac
    21601 gaaactggac ggcacggcga aaggcggggt aattttctcg gtggctgacc agtttggtat
    21661 ccctatccgc tacattggtg tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga
    21721 cgactttata gaggcacttt ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc
    21781 agcaaggctt atctcggtgg gagacaggcg ctgcagggcg ttacgttcca tatgcagccg
    21841 ggtgagatgg cgtttctgat cggtcattcc ggcgcaggga aaagtaccct cctgaagctg
    21901 atctgtggga ttgagcggcc cagcgccggg aaaatctggt ttagcggcca tgacatcacg
    21961 cgtctgaaaa accgtgaagt tccgtttctg cgccgccaga ttggcatgat tttccaggat
    22021 caccatctac tgatggaccg tactgtctat gataacgtgg cgatcccgct gattatcgcc
    22081 ggtgccagcg gtgatgacat tcgtcgccgg gtgtcggcgg cgctggataa agtcgggcta
    22141 ctggacaaag cgaagaactt ccctattcag ctttccggcg gtgaacaaca gcgtgttggc
    22201 attgcccgcg cggtggtgaa caagcccgcg gtactgctgg cggacgaacc gactggtaac
    22261 ctggacgacg cgctgtcgga aggcatttta cgtctgtttg aagagtttaa ccgcgttgga
    22321 gtaaccgtat tgatggcaac gcacgacatc aacctgattt cgcgacgttc ttatcgcatg
    22381 ctcaccctga gcgatggtca cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat
    22441 caatcacatt cggcagtttg gcgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc
    22501 tggcgacggc ggtcgtaacg cgccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg
    22561 caaaaccaac gttttcaacg aacaggtacg ctatgccttc cacggcgcat tgcaggatct
    22621 gaaaagcaaa ccgttcgcca cgtttttaac ggtgatggtt atcgccatct ccctgacgct
    22681 gcccagcgtc tgttatatgg tgtacaaaaa cgttaaccag gcggcgacgc aatattatcc
    22741 gtcaccgcaa atcactgttt atctgcaaaa aacgctggat gatgacgccg ctgcgggcgt
    22801 ggtggcacag ttgcaggccg agcaaggcgt ggagaaagtg aattatcttt ctcgtgaaga
    22861 cgcactgggc gagttccgta actggtctgg ttttggtggt gcgctggata tgctggaaga
    22921 aaacccgctt ccggcagtag cggtggtgat cccgaaactc gattttcagg ggacggagtc
    22981 actgaatacg ctgcgtgatc gtatcacgca gattaacggc attgacgaag tgcggatgga
    23041 tgacagctgg tttgcccgcc tggcggcgtt aaccgggctg gtcgggcgcg tttcggcgat
    23101 gatcggcgtg ttgatggtgg cagcagtgtt tctcgtcatc ggcaacagtg tgcgtctgag
    23161 catctttgct cgccgtgact ctattaacgt gcagaaactg attggtgcga cggatggttt
    23221 catcctgcgc ccgttcctgt acggtggcgc actgctggga ttttctggcg cattgttgtc
    23281 attaattttg tcagaaattc tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca
    23341 ggttttcgga acgaagtttg atatcaatgg tttatcgttc gatgaatgcc tgctattgct
    23401 gctggtatgc tcgatgattg gctgggtggc agcgtggctt gccacggtac aacatttacg
    23461 ccactttacg cctgaataat aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag
    23521 cagggaaaga gaccccgttg tctcttcccg gtatttcatc tctatgtcac attttgtgcg
    23581 taatttattc acagcgttgc attgaacttg tggataaaat cacggtctga taaaacagtg
    23641 aatgataacc tcgttgctct taagctctgg cacagttgtt gctaccactg aagcgccaga
    23701 agatatcgat tgagaggatt tgaatgactg acaaaatgca aagtttagct ttagccccag
    23761 ttggcaacct ggattcctac atccgggcag ctaacgcgtg gccgatgttg tcggctgacg
    23821 aggagcgggc gctggctgaa aagctgcatt accatggcga tctggaagca gctaaaacgc
    23881 tgatcctgtc tcacctgcgg tttgttgttc atattgctcg taattatgcg ggctatggcc
    23941 tgccacaggc ggatttgatt caggaaggta acatcggcct gatgaaagca gtgcgccgtt
    24001 ttaacccgga agtgggtgtg cgcctggtct ccttcgccgt tcactggatc aaagcagaga
    24061 tccacgaata cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc aaagcgcaac
    24121 gcaaactgtt cttcaacctg cgtaaaacca agcagcgtct gggctggttt aaccaggatg
    24181 aagtcgagat ggtggcccgt gaactgggcg taaccagcaa agacgtacgt gagatggaat
    24241 cacgtatggc ggcacaggac atgacctttg acctgtcttc cgacgacgat tccgacagcc
    24301 agccgatggc tccggtgctc tatctgcagg ataaatcatc taactttgcc gacggtattg
    24361 aagatgataa ctgggaagag caggcggcaa accgcctgac cgatgcgatg caaggtctgg
    24421 acgagcgtag tcaggatatc atccgcgcgc gctggctgga tgaagacaac aagtccacgt
    24481 tgcaggaact ggctgaccgt tacggtgttt ctgctgagcg tgtgcgccag ctggaaaaga
    24541 acgcgatgaa aaaattgcgc gctgctattg aagcgtaatt tccgctatta agcagagaac
    24601 cctggatgag agtccggggt ttttgttttt tgggcctcta caataatcag tttccccctc
    24661 cggcaaaacg ccaatcccca cgcagattgt taataaactg tcaaaatagc tattccaata
    24721 tcataaaaat ggggtatgtt ttagcagagt atgctgctaa agcacgggta gctacgtata
    24781 aaacgaaata aagtgctgca caacaacatc acaacacacg taataaccag aagaatgggg
    24841 attctcagga tgaacataaa gggtaaagcg ttactggcag gatgtatcgc gctggcattc
    24901 agcaatatgg ctctggcaga agatattaaa gtcgccgtcg taggcgcaat gtccggtccg
    24961 gtggcgcagt acggtgacca ggagtttact ggcgcagagc aggcggttgc ggatatcaat
    25021 gctaaaggtg gtatcaaagg caacaaactg caaatcgtaa aatatgacga tgcctgtgac
    25081 ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa atacgtgatt
    25141 ggtcacctct gctcttcatc aacgcagcca gcgtctgaca tctacgaaga cgaaggcatt
    25201 ttaatgatca ccccagcggc aaccgcacca gagctgaccg cccgtggcta tcagctgatc
    25261 ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata tattcttgag
    25321 aaagtgaaac cgcaacgcat tgctatcgtt catgacaaac agcagtacgg cgaaggtctg
    25381 gcgcgtgcag tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt ctttgatggc
    25441 atcaccgccg gggaaaaaga tttctctacc ctggtggcgc gtctgaagaa agagaatatc
    25501 gacttcgttt actacggtgg ttatcacccg gaaatggggc aaatcctgcg tcaggcacgc
    25561 gcggcagggt tgaaaactca gtttatgggg ccggaaagtg tggctaacgt ttcgctgtct
    25621 aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa ctacgatcag
    25681 gttccggcga acaaacccat tgttgatgcg atcaaagcga aaaaacagga cccaagcggc
    25741 gcattcgtct ggaccaccta cgccgcgctg caatctttgc aggcgggcct gaatcagtct
    25801 gacgatccgg cagaaatcgc caaatacctg aaagcgaact ccgtggatac cgtgatggga
    25861 ccgctgacct gggatgagaa aggcgatctg aaaggctttg agttcggcgt atttgactgg
    25921 cacgctaacg gcacggcgac cgatgcgaag taatcattaa tcggcaactt tgggttgccg
    25981 ccaaattgct aatatcgagt acgttgcttc atgccggatg cggcgtaaac gccttatccg
    26041 gcctacaaga ttcaaagaaa tcagtaaatt gcagcacaca ttgtaggcct gataagcgta
    26101 gcgcatcagg caatatcact tgtgaaatca aacttgacga ttaacgcttc tcccagccgc
    26161 cctgttgtgc cgtaaatccc agtgcctgca taaacgctgt catcacgccg cgatcttcca
    26221 cgcctgcatc cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca
    26281 gcagatattg ccccacaccg cgacggcggg tgacttccct cacacgcagg gaatccagtg
    26341 ccccctcggt gccgcttaag gttacccgca cggccgcgag aaggcgctcg ttaaaacgcg
    26401 cggcgtagat acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga
    26461 tcttttgcag gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct
    26521 tcatggggaa cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg
    26581 aggctttctc ttttttaatt cttttggcag gtgattaatt ttttaacagc aataattaca
    26641 aagttaaaac attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta
    26701 ttccgccctt tgtaccgtta ttttatgctg acaacggcgc ttttttctgt ttatctatca
    26761 ataaattcag aatattatct gttcttaatc gactgaaaaa tagggatttt aatcgctatc
    26821 atcacaaaat actgtgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa
    26881 agaacagtct gatttgttaa cacataagaa caaagcaaca caacatcacg aatggggatt
    26941 tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc
    27001 acacaccgct atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat
    27061 tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag gcaattaaag acattaatgc
    27121 caaaggggga attaagggcg ataaactggt tggcgtggaa tatgacgacg cctgcgaccc
    27181 gaaacaagcc gttgcggtcg ccaacaaaat cgttaacgac ggcattaaat atgttattgg
    27241 tcatctgtgt tcctcctcta cccaacctgc atcagatatc tatgaagacg aaggtattct
    27301 gatgatctcg ccgggagcga ccaatccgga gctgacccaa cgcggttatc aatacatcat
    27361 gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac
    27421 ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc
    27481 acgttcggtg caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggcat
    27541 taccgctgga gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga
    27601 cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc
    27661 cgttggcctg aaaacccagt ttatggggcc ggaaggtgtc ggtaatgcgt cactgtcgaa
    27721 cattgccggt gatgccgctg aaggcatgtt ggtcaccatg ccaaaacgct atgaccagga
    27781 tccgacaaac cagggcatcg ttgatgcgct gaaagccgac aagaaagatc cgtccgggcc
    27841 ctatgtctgg atcacctacg cggcggtgca atctctggca actgcccttg aacgtaccgg
    27901 cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtaat
    27961 tgggccgctg aactgggatg aaaaaggcga tcttaaggga ttcgattttg gtgtcttcca
    28021 gtggcacgcc gacggttcat ccacggcagc caagtgatca tcctaccgtc cgtgaaaaac
    28081 gggcgggttt agaaaggtta ccttatgtct gagcagtttt tgtatttctt gcagcagatg
    28141 tttaacggcg tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt
    28201 tacggcatta tcggcatgat taacttcgcc cacggcgagg tttacatgat tagtagctat
    28261 gtctcattta tgatcatcgc cgcactgatg atgatgggta ttgataccgg ctggctgctg
    28321 gtggcggcgg gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa
    28381 cgggtagcct atcgcccggt gcgtaactct aagcgcctga ttgcgcttat ttccgcaatc
    28441 ggcatgtcta tcttcctgca aaactacgtc agcctgaccg aaggttcgcg cgacgtggcg
    28501 ctgcccagcc tgtttaacgg tcagtgggta gtgggtcata gcgaaaactt ctctgcctct
    28561 attactacca tgcaggcggt tatctggatt gttactttcc tcgccatgct ggcactgacg
    28621 attttcattc gctattcccg tatggggcga gcctgccgtg cctgcgcgga agatctgaaa
    28681 atggcgagcc tgcttggcat caacaccgac cgggtgattg cgctgacctt tgtgattggc
    28741 gcggcgatgg cggcggttgc gggcgtgctg ctcggtcagt tctacggcgt aattaacccc
    28801 tacatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc
    28861 agcattccgg gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct
    28921 gcctatctga gtacggaata taaagatgtg gtctcattcg ccctgctgat cctggtgctg
    28981 ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc
    29041 atattgcaat ggcgctgctc tctgccgcta tgttctttgt gctggcgggc gtctttatgg
    29101 gcgtgcaact ggagctggat ggcaccaaac tggtggtcga cacggcttca gacatccgtt
    29161 ggcagtgggt gtttatcggc acggcggtgg tctttttctt ccagcttgtg cggcctgctt
    29221 tccagaaagg gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct
    29281 ccacggtgaa gcagaaactg ttcctcgtgg cgctgctggt gcttgcagtg gcgtggccgt
    29341 ttatggtgtc gcgtgggacg gtggatatcg ccactctgac catgatctac attattctcg
    29401 gtctcgggct gaacgtggtt gttggtcttt ctggtctgct ggtgctggga tacggcggct
    29461 tttacgccat cggcgcttac acttttgcgc tgctcaatca ctattacggc ctgggcttct
    29521 ggacctgtct gccgattgcc gggttaatgg cggcggcggc gggcttcctg ctcggttttc
    29581 cggtgctgcg tctgcgcggt gactatctgg cgatcgttac cctcgggttc ggcgaaattg
    29641 tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg accgaacggt atcagtcaga
    29701 ttccgaaacc gacgctcttc ggactcgagt tcagccgtac cgcccgcgaa ggcggctggg
    29761 acacgttcag caatttcttt ggcctgaaat acgatccctc cgatcgcgtc attttcctct
    29821 atctggtggc gttgctgcta gtggtgttaa gcctgtttgt cattaaccgc ctgctgcgga
    29881 tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagatga aatcgcttgt cgttcgctgg
    29941 gcttaagccc gcgtcgtatc aagctgactg cctttaccat cagcgctgcg tttgccggtt
    30001 ttgccggaac gttgtttgct gcgcgccagg gctttgtcag ccctgaatcc ttcacttttg
    30061 ccgaatcggc gtttgtgctg gcgatagtgg tgctcggcgg tatgggctcg caatttgcag
    30121 tgattctggc ggctgttttg ctggtggtat cgcgcgagtt gatgcgtgat ttcaacgaat
    30181 acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct
    30241 tgctgcccat gacgcgcccg caactcaagc tgaaaaacgg cgcagcaaaa ggagagcagg
    30301 catgagtcag ccattattat ctgttaacgg cctgatgatg cgcttcggcg gcctgctggc
    30361 ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa
    30421 cggtgccgga aaaaccacgg tcttcaactg cctgaccgga ttctataaac ccaccggcgg
    30481 caccatttta ctgcgcgatc agcatctgga aggtttaccg gggcagcaaa ttgcccgcat
    30541 gggcgtggtg cgcaccttcc agcatgtacg tctgttccgt gaaatgacgg tgattgaaaa
    30601 cctgctggtg gcacagcatc agcaactgaa aactgggctg ttctccggcc tgttgaaaac
    30661 cccctctttc cgtcgcgccc agagcgaagc gctcgaccgc gccgctacct ggcttgagcg
    30721 cattggtttg ctggaacatg ccaaccgtca ggcgagtaac ctggcctatg gtgaccagcg
    30781 tcgtctggag attgcccgct gtatggtaac gcagccggag attttaatgc tcgacgaacc
    30841 ggcagcaggt cttaacccga aagagacgaa agagctggat gagctgattg ccgaactgcg
    30901 taaccatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat
    30961 ttcagacaga atttacgtgg tcaatcaggg gacgccgctg gcaaacggta caccggagca
    31021 gatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag
    31081 tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg
    31141 tgagcctgca tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga
    31201 aaaccacctt gctcggcacg ttatgcggcg atccgcgcgc caccagcggg cgaattgtgt
    31261 ttgatgataa agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga
    31321 ttgtcccgga agggcgtcgt gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg
    31381 gcggcttttt tgccgaacgc gaccagttcc aggagcgcat aaagtgggtc tatgaactgt
    31441 ttccacgtct gcatgagcgc cgtgttcagc gagcgggcac tatgtctggc ggtgaacagc
    31501 agatgctggc gattggtcgc gcgctgatga gcaacccgcg tttgttgttg cttgatgagc
    31561 catcgctcgg gctggcaccg attatcatcc agcaaatttt cgacaccatc gaacaactgc
    31621 gcgagcaggg gatgaccatc ttccttgtcg agcagaacgc caaccaggcg ctaaagctgg
    31681 cggatcgcgg ctacgtgctg gaaaacggtc atgtagtgct ctccgatacc ggcgacgcgc
    31741 tgctggcgaa tgaagcagtg agaagtgcat atttaggcgg gtaataacac gttgattgat
    31801 aaggagtcaa aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg
    31861 cttatcaggc ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga
    31921 taaggcgttt acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga
    31981 ccttctattt ttccgcttat ccgtgcccca tctcccattt tccctcgccc gcgccgtcac
    32041 cgccttgtca tctttctgac accttactat cttaaaaatg taacaaaata gttatttttc
    32101 tgtaattcga gcatgtcatg ttactccgcg agcataaaac gcgtgaattc gcgcattcgg
    32161 tacaacaaga gagataatca atgaaaccgt tacgttatac agcttcagca ctggcgctcg
    32221 gactggcttt aatggcaaat gcgcaggcag tgacgaccat tccgttctgg cattctatgg
    32281 aaggggaact gggtaaagag gtggattctc tggcccaacg ttttaacgcc gaaaacccgg
    32341 attacaaaat tgtaccgacc tataaaggca actacgaaca gaatttaagc gcggggattg
    32401 ccgcatttcg taccggcaac gctccggcta ttttgcaggt ttatgaagtt ggcaccgcca
    32461 ccatgatggc gtcgaaagcc attaaaccgg tgtatgacgt atttaaagag gcggggattc
    32521 aattcgatga gtcgcagttt gtgccgacgg tttcaggcta ttactccgac agcaaaaccg
    32581 gccacttact ctcccagccg ttcaacagtt cgacccccgt tctctattac aacaaagacg
    32641 ccttcaagaa agcaggatta gacccggaac aaccgccgaa aacctggcag gatctggcgg
    32701 actatgccgc aaaactaaaa gcctccggca tgaagtgcgg ctacgccagc ggctggcagg
    32761 gatggatcca actggaaaac tttagcgcct ggaacggtct gccgtttgcc agcaaaaaca
    32821 acggctttga cggcacagac gcggtgctgg agttcaacaa gccggagcag gtgaaacaca
    32881 tcgccatgct cgaagagatg aacaagaagg gcgatttcag ctacgttggg cgtaaggatg
    32941 aatccaccga gaagttctat aacggtgatt gcgcgatgac gaccgcctct tccggttctc
    33001 ttgccaacat tcgcgagtac gccaaattta actatggcgt aggcatgatg ccttacgacg
    33061 ccgatgcgaa agacgcgccg caaaacgcca ttatcggcgg agccagtcta tgggtaatgc
    33121 agggtaaaga taaagaaacc tacaccggcg tggcgaagtt cctcgacttc ctcgcaaaac
    33181 cagaaaacgc tgccgagtgg catcagaaaa ccggctatct gccaatcacc aaagcggcgt
    33241 atgacctgac ccgtgagcag ggcttttacg agaaaaaccc aggagcggat attgccacgc
    33301 gtcagatgct gaacaagccg ccgttgccgt tcaccaaagg gctgcgtctg ggcaacatgc
    33361 cgcagatccg cgtgattgtg gatgaagagc tggagagcgt gtggaccggt aagaagacac
    33421 cacagcaggc gctggatact gccgttgagc gtgggaacca gttactgcgc cgctttgaga
    33481 aatcgacgaa gtcttaatca gtgtaatgtc ggatgcgttt cgcttatctg acctggcatc
    33541 gcgtgtaggc cggataagcg aagcgcatcc ggcacagttc aggaattaac tgtaatgtca
    33601 tcatcccgtc cggtgttccg ctcgcgctgg ctgccttatt tgctggtcgc gccgcagctc
    33661 atcatcaccg ttatcttttt tatctggcct gcgggcgaag cgttgtggta ctcgctacaa
    33721 agcgtcgatc cgtttggttt ctccagccag tttgtcggcc tggataactt cgtcacgctg
    33781 tttcacgaca gctactatct cgacgccttc tggacgacga taaaattcag cacctttgtc
    33841 accgtcagcg gtttgctggt atcgctgttc ttcgcagcgc tggtggagta catcgtgcgc
    33901 ggcagccgtt tctatcaaac cttgatgttg ctaccgtatg ccgtggctcc cgccgttgcc
    33961 gcggtattgt ggatcttcct gtttaacccc ggtcgcgggc tgattactca ttttctcgct
    34021 gagttcggct acgactggaa ccacgcgcaa aacagcggtc aggcgatgtt cctggtggtg
    34081 tttgcctcag tgtggaagca aatcagctac aactttctgt tcttctatgc cgcgctgcaa
    34141 tccatccccc gttcgttgat cgaagccgca gccattgatg gggctggccc gattcgccgc
    34201 ttctttaaga ttgcgctgcc gcttatcgct ccggtgagtt tcttcctgct ggtggtgaac
    34261 ctggtgtatg ccttcttcga caccttcccg gtgatcgacg ccgccacgtc cggcgggcca
    34321 gtacaggcca ccacgacgct gatttataag atttaccgtg aaggctttac cggactggat
    34381 ttggcttcgt ctgccgcgca gtcggtggtg ttgatgttcc tcgtcatcgt gctgacggtg
    34441 gtgcagttcc gctatgttga aagcaaggta cgttaccaat gattgagaac cgtccgtggc
    34501 tgacaatatt cagccatacc atgctgatcc tcgggatcgc ggtgatcctc ttcccgctgt
    34561 acgtggcgtt tgtcgcggcg acgctggata aacaagaagt ctatgccgca ccgatgacgc
    34621 taatccccgg cacacatctg ctggaaaaca tccacaacat ctgggtaaac ggggtaggca
    34681 cgaatagcgc gccgttctgg cggatgttgc ttaacagctt tgtgatggcg ttcagcatta
    34741 cgctcggcaa aattaccgtc tcgatgctct cggcatttgc cattgtctgg tttcgttttc
    34801 cgctacgtaa cctcttcttc tggatgattt ttatcaccct gatgctgccg gttgaagtgc
    34861 gtatcttccc gacggtggaa gtcatcgcca acctgaaaat gctcgacagc tacgcaggtt
    34921 taacgctgcc gctgatggcc tcggcgaccg ccacgtttct gtttcgccag ttctttatga
    34981 ccctaccgga tgaactggtg gaagccgcgc ggatcgacgg cgcgtcgcca atgcgtttct
    35041 tttgcgacat cgtttttccg ctgtccaaaa ccaatctggc ggcgctgttt gtgatcacct
    35101 ttatttacgg ctggaaccag tatttgtggc cgttgttgat tattaccgac gttgatctcg
    35161 gcaccaccgt ggcagggatt aaagggatga tcgccacagg cgaaggcaca acggaatgga
    35221 actcggtgat ggcggcgatg ttgttaaccc tgatcccacc agtagtgatt gttttagtga
    35281 tgcagcgtgc cttcgtgcgc ggcctggtcg atagtgagaa ataagatggc aggactgaaa
    35341 ttacaggcag taaccaaaag ctgggatggc aaaacccagg tcattaaacc gctgaccctt
    35401 gatgtggcgg atggcgaatt tatcgtgatg gtcgggccgt ctggctgcgg gaaatcgacg
    35461 ttattgcgaa tggttgccgg actggagcgg gtaacgacgg gcgatatctg gatcgaccgc
    35521 aagcgtgtca ccgaaatgga gccgaaagat cgcgggattg cgatggtgtt ccagaactac
    35581 gcgctctatc cgcatatgag cgtagaggag aacatggcgt gggggctgaa aattcgcggc
    35641 atgggcaagc aacaaattgc cgaacgtgtt aaagaagcgg cgcgcattct ggagctggac
    35701 ggcctgctta agcgccgccc gcgtgagcta tccggcggtc agcgtcagcg tgtggcgatg
    35761 gggcgtgcga ttgtgcgtga gccggcagtg ttcctgtttg atgagccgct ctctaacctc
    35821 gatgccaagt tgcgtgtgca gatgcgtctt gaactgcaac agctgcaccg tcgcctgaaa
    35881 acgacttcac tctacgttac ccacgatcag gttgaggcga tgacgctcgc ccagcgagta
    35941 atggtgatga acggcggcgt cgccgaacag attggcacgc cagttgaagt gtacgaaaag
    36001 cccgccagcc tgtttgtggc gagttttatc ggcagcccgg cgatgaacct actggctggc
    36061 cgcgtgaata acgaaggtac gcatttcgaa ctggatggtg gtattacgct gccgctaaac
    36121 ggtggctacc gtcagtatgc cgggcgtaaa atgaccctcg gcattcgccc ggaacatata
    36181 gcgctaagct cgcaggcaga aggtggtgtg ccgctggtga tggacacgct ggagatcctc
    36241 ggcgcagata acctggcgca cggacgttgg ggcgaacaga agctggtggt gcgactggca
    36301 catcaggagc gcccgacggc aggcagcacg ctgtggctgc atctgccgga aaatcagctg
    36361 catctttttg atggtgaaac aggacaacga gtatgagtaa ctggccttat ccccgcatcg
    36421 tcgcccatcg tggtggcggt aagctggccc cggagaacac cctggcggca atcgacgtcg
    36481 gggcaaaata cggtcataag atgatcgaat ttgacgcgaa gttatcgaaa gatggcgaga
    36541 tctttctgct ccatgacgac aatctcgaac gtaccagcaa cggttggggc gttgcgggcg
    36601 agctgaactg gcaggattta ctgcgcgtgg atgcaggcag ttggtacagc aaagcgttta
    36661 aaggtgagcc gctgccgttg ctttcgcagg tggcggaacg ctgccgcgaa cacgggatga
    36721 tggcgaatat cgaaatcaaa cccaccactg gcactggacc attaacgggc aaaacggtgg
    36781 cgttggcggc acgcgaactg tggaccggta tgacgccgcc gctgctgtca tcgtttgaga
    36841 ttgatgcttt agaagccgcg caacaggcag caccggaact gccgcgtggt ttattgcttg
    36901 atgaatggcg cgacgactgg cgagaattga ccgcgcgact gggctgcgtc tctattcatc
    36961 tcaatcataa gttgctcgat aaagcgcgag tgatgcagtt gaaagatgcc ggattacgga
    37021 ttctggttta taccgtcaat aaaccccagc gcgcggcaga gttgctgcgc tggggcgtgg
    37081 attgcatctg taccgatgcg attgacgtga ttggtccgaa ctttacggcc caatagtttt
    37141 caacggaatg tcaggctgcg gcgtgctggg ctgattcagc atgtcgccgt ttctcctctc
    37201 cggcagcata tgctgctgat tcaacgaact atccggatta cggttgctgt ttaacatccc
    37261 gccgttggtg ttgggcagca tttgctgccg ggcgggattt cgctcccccg gctgtgactg
    37321 caacacccgc tgagaattat tgtttatctg gttttctaaa tgctgctgtt gcagctgcgt
    37381 ttgcgttttc agttgctggt tcagcatccc tttttgctgg atttgctgcg tctgcatttg
    37441 agtttgcatc cgctgctggc tggggatctg atatcccggc tggttagggt tgttcagagt
    37501 attaatgggc tgtgcaaagc cgacaaacgg caggagtgcc gtcaaaagca gaagtcgttt
    37561 catcgcgtat cctcctctga agatattctt taagtttact cgcttcccgg caaaacgatg
    37621 attaattcag agttatatac caggcttagc tggggttgcc ccttaatctc tggagaataa
    37681 cgatgataaa accgacgttt ttacgccggg tggccattgc tgctctgctc acaggaagtt
    37741 gttttagtac ggtagccgcg ccacctgttt cttatggtgt ggaggaagat gtctttcatc
    37801 cggtacgcgc taagcaggga atggtggcgt ctgtagatgc tactgccact caggtggggg
    37861 tggatatcct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg ggctacgcgc
    37921 tggcagtaac gcatccgcag gcagggaatc tgggcggcgg tggttttatg ctgatccgct
    37981 cgaaaaatgg caataccacg gctatcgatt tccgcgaaat ggcacccgcc aaagcgaccc
    38041 gcgatatgtt cctcgatgat cagggcaacc cggacagcaa aaaatcactc acttcgcatc
    38101 tggcttccgg cacacccggt acggtagcgg gtttctcgtt ggcgctggat aaatacggca
    38161 ccatgccgtt gaataaagtg gtgcaaccgg cgtttaaact ggcacgcgat ggttttatcg
    38221 ttaacgacgc gctggctgac gatctcaaaa cctacggcag tgaagtgctg ccgaaccacg
    38281 aaaacagcaa agctatcttc tggaaagagg gcgaaccgct gaaaaagggc gacaagctgg
    38341 tgcaggcgaa cctggcaaag agcctggaga tgattgccga aaacggcccg gatgaattct
    38401 acaaaggcac gatagcggaa cagattgccc aggagatgca gaaaaacggt ggcttgatca
    38461 gcaaagaaga tttagcggcc tataaagcgg tcgaacgcac accgataagc ggcgattatc
    38521 gcgggtatca ggtttactcc atgccaccgc catcttccgg cggtattcat atcgtacaaa
    38581 tcctcaatat tctggaaaac ttcgatatgc agaagtacgg ctttggcagc gccgacgcga
    38641 tgcaaatcat ggcagaagct gagaaatacg cctatgccga ccgctcggaa tatcttggcg
    38701 acccggattt tgtcaaagta ccgtggcagg cgctgaccaa taaagcctat gccaaatcta
    38761 ttgccgatca aattgatatc aataaagcga agccattcag cgaaattcgt cctggcaagc
    38821 ttgcgcctta tgagagtaat caaactaccc attactcagt agtggataaa gacggtaacg
    38881 ccgtggcggt gacctatacg ctgaacacca ccttcggtac gggcattgtc gcgggcgaga
    38941 gcggtatttt gctcaataac cagatggatg atttctccgc caaaccaggc gtaccgaacg
    39001 tttacgggct ggtgggcggc gatgccaacg ccgtcgggcc gaataaacgc cctctgtcgt
    39061 cgatgtcacc aaccattgtg gtcaaggatg gtaaaacctg gctggtcaca ggaagcccag
    39121 gcggtagccg gattatcacc actgtgctgc aaatggtggt gaatagcatc gattatggaa
    39181 tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg ccggacgagc
    39241 tgcgtgtcga aaaagggttt agcccggata cgatcaagct gctggaagca aaaggtcaga
    39301 aagtggcgct gaaagaggcg atgggcagta cacaaagcat tatggttggg ccggacggtg
    39361 agctgtacgg tgcatccgac ccgcgctcgg tggatgattt aacggcgggg tattaaggtt
    39421 agcggccctc ttagtgggaa gagggccgta ttgtcagggc aagccgaagg tagccttttt
    39481 tatttcataa tcctgtagat attcttccag tggctttgtc gttcccagag aatgaatttc
    39541 accactgcct ttatcaacaa taaaaggtgc gttaccagct aagcgcgcgg cctcatctcc
    39601 agtttcgaga aattctcgtg cttcgaaaca gaaataccag ccctggctaa agcgtccatg
    39661 tagagtaatg acgaccggga gatttgcatc atcgaggtaa ttgttcgctt ccgcgaatgc
    39721 gtcgtgataa gtaatcataa ttataataaa tattattgtt tagcgttata ttattattcc
    39781 tgcgttatac attgagcaaa gacgcgttca tcttcgtcaa agatattatt aaatgctggt
    39841 tttgaaaaga gttcatcgga taaggtatag tcacaaaata agtatctgct aatattttgt
    39901 gatttatttt ccagaacgtc gaaccggatt tgctcattta agatatcaga atcttatgat
    39961 tcgggtaaaa gtctaaccaa atcaacatgt cactaagatc atcatcgcca ttagctattt
    40021 cagtttaaat agcgactaca tgtcttcggt ccatctcact taaggagtgt agttccgttg
    40081 taagtttttc tatagcttgt attgataaat ttcgatcaag aaatttactg ctggtaaact
    40141 ctaaaaagat ggcatggttt acaatgattt tggttacctt tttattatta tgaacaactg
    40201 tccatgattt cgtttaagaa tgaagagaaa tcactaaac
//
LOCUS       DAZFRE010000035        39368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFRE010000035 DAZFRE010000000
VERSION     DAZFRE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.035"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            136..702
                     /locus_tag="ACHKUI_002168"
     CDS             136..702
                     /locus_tag="ACHKUI_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598355.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
     gene            783..3431
                     /locus_tag="ACHKUI_002169"
     CDS             783..3431
                     /locus_tag="ACHKUI_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6598356.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            3451..4203
                     /locus_tag="ACHKUI_002170"
     CDS             3451..4203
                     /locus_tag="ACHKUI_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6598357.1"
                     /translation="MSYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HSLPSDRESLFYYNVREIPPKTGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            4220..4723
                     /locus_tag="ACHKUI_002171"
     CDS             4220..4723
                     /locus_tag="ACHKUI_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598358.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSVVEFGNVIIKSIDGANYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAVDHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            4720..5199
                     /locus_tag="ACHKUI_002172"
     CDS             4720..5199
                     /locus_tag="ACHKUI_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598359.1"
                     /translation="MKRLAWCLIYGFAGLVQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            5196..5723
                     /locus_tag="ACHKUI_002173"
     CDS             5196..5723
                     /locus_tag="ACHKUI_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598360.1"
                     /translation="MNLKRISHCAAALATVLLTPHVQAADNLHFYGNLLSKSCTLVVQ
                     GESLADVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDASSGAQGVGIGMEKTDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTASLTATFEYI"
     gene            5725..6582
                     /locus_tag="ACHKUI_002174"
     CDS             5725..6582
                     /locus_tag="ACHKUI_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO6598361.1"
                     /translation="MKITNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNSQV
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTTVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDENYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVTPNRHPITDKQLHDMVVHVDTSSRQPIDSTC
                     RFQYTLNEL"
     gene            complement(6902..7453)
                     /gene="smrB"
                     /locus_tag="ACHKUI_002175"
     CDS             complement(6902..7453)
                     /gene="smrB"
                     /locus_tag="ACHKUI_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6598362.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYDGDAALLVLIEVDEWLPPELP"
     gene            7619..8551
                     /gene="prmB"
                     /locus_tag="ACHKUI_002176"
     CDS             7619..8551
                     /gene="prmB"
                     /locus_tag="ACHKUI_002176"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6598363.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAVREHFAIYKD"
     gene            8586..9671
                     /gene="aroC"
                     /locus_tag="ACHKUI_002177"
     CDS             8586..9671
                     /gene="aroC"
                     /locus_tag="ACHKUI_002177"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6598364.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRRESDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9675..10499
                     /gene="mepA"
                     /locus_tag="ACHKUI_002178"
     CDS             9675..10499
                     /gene="mepA"
                     /locus_tag="ACHKUI_002178"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6598365.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10499..11308
                     /locus_tag="ACHKUI_002179"
     CDS             10499..11308
                     /locus_tag="ACHKUI_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6598366.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPVLVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11308..11856
                     /gene="epmC"
                     /locus_tag="ACHKUI_002180"
     CDS             11308..11856
                     /gene="epmC"
                     /locus_tag="ACHKUI_002180"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6598367.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11890..12168
                     /locus_tag="ACHKUI_002181"
     CDS             11890..12168
                     /locus_tag="ACHKUI_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6598368.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(12289..14295)
                     /gene="mnmC"
                     /locus_tag="ACHKUI_002182"
     CDS             complement(12289..14295)
                     /gene="mnmC"
                     /locus_tag="ACHKUI_002182"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6598369.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSKLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIHYQHQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14454..15674
                     /gene="fabB"
                     /locus_tag="ACHKUI_002183"
     CDS             14454..15674
                     /gene="fabB"
                     /locus_tag="ACHKUI_002183"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6598370.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15939..17117
                     /locus_tag="ACHKUI_002184"
     CDS             15939..17117
                     /locus_tag="ACHKUI_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6598371.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATMFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17114..18109)
                     /gene="flk"
                     /locus_tag="ACHKUI_002185"
     CDS             complement(17114..18109)
                     /gene="flk"
                     /locus_tag="ACHKUI_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6598372.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18208..19344
                     /gene="pdxB"
                     /locus_tag="ACHKUI_002186"
     CDS             18208..19344
                     /gene="pdxB"
                     /locus_tag="ACHKUI_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6598373.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LNELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19410..20423
                     /locus_tag="ACHKUI_002187"
     CDS             19410..20423
                     /locus_tag="ACHKUI_002187"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6598374.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFILTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGSPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20423..21235
                     /gene="truA"
                     /locus_tag="ACHKUI_002188"
     CDS             20423..21235
                     /gene="truA"
                     /locus_tag="ACHKUI_002188"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6598375.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21318..21977
                     /locus_tag="ACHKUI_002189"
     CDS             21318..21977
                     /locus_tag="ACHKUI_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6598376.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            22133..23047
                     /gene="accD"
                     /locus_tag="ACHKUI_002190"
     CDS             22133..23047
                     /gene="accD"
                     /locus_tag="ACHKUI_002190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6598377.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            23117..24385
                     /gene="folC"
                     /locus_tag="ACHKUI_002191"
     CDS             23117..24385
                     /gene="folC"
                     /locus_tag="ACHKUI_002191"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6598378.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEMIDARRSGGK"
     gene            24375..25037
                     /gene="dedD"
                     /locus_tag="ACHKUI_002192"
     CDS             24375..25037
                     /gene="dedD"
                     /locus_tag="ACHKUI_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6598379.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25569..26057
                     /gene="cvpA"
                     /locus_tag="ACHKUI_002193"
     CDS             25569..26057
                     /gene="cvpA"
                     /locus_tag="ACHKUI_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6598380.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            26094..27611
                     /gene="purF"
                     /locus_tag="ACHKUI_002194"
     CDS             26094..27611
                     /gene="purF"
                     /locus_tag="ACHKUI_002194"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6598381.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27706..28275
                     /gene="ubiX"
                     /locus_tag="ACHKUI_002195"
     CDS             27706..28275
                     /gene="ubiX"
                     /locus_tag="ACHKUI_002195"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6598382.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            28541..29323
                     /gene="argT"
                     /locus_tag="ACHKUI_002196"
     CDS             28541..29323
                     /gene="argT"
                     /locus_tag="ACHKUI_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6598383.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            29544..30326
                     /gene="hisJ"
                     /locus_tag="ACHKUI_002197"
     CDS             29544..30326
                     /gene="hisJ"
                     /locus_tag="ACHKUI_002197"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6598384.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            30416..31102
                     /gene="hisQ"
                     /locus_tag="ACHKUI_002198"
     CDS             30416..31102
                     /gene="hisQ"
                     /locus_tag="ACHKUI_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6598385.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            31099..31815
                     /locus_tag="ACHKUI_002199"
     CDS             31099..31815
                     /locus_tag="ACHKUI_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598386.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31823..32596
                     /gene="hisP"
                     /locus_tag="ACHKUI_002200"
     CDS             31823..32596
                     /gene="hisP"
                     /locus_tag="ACHKUI_002200"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6598387.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32792..33682
                     /gene="rpnB"
                     /locus_tag="ACHKUI_002201"
     CDS             32792..33682
                     /gene="rpnB"
                     /locus_tag="ACHKUI_002201"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6598388.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(33730..34623)
                     /locus_tag="ACHKUI_002202"
     CDS             complement(33730..34623)
                     /locus_tag="ACHKUI_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6598389.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDKELRG
                     PFNMVSPYPVRNEQFAHTLGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34644..35006)
                     /gene="folX"
                     /locus_tag="ACHKUI_002203"
     CDS             complement(34644..35006)
                     /gene="folX"
                     /locus_tag="ACHKUI_002203"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6598390.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            complement(35063..35710)
                     /gene="yfcG"
                     /locus_tag="ACHKUI_002204"
     CDS             complement(35063..35710)
                     /gene="yfcG"
                     /locus_tag="ACHKUI_002204"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6598391.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            35846..36490
                     /gene="yfcF"
                     /locus_tag="ACHKUI_002205"
     CDS             35846..36490
                     /gene="yfcF"
                     /locus_tag="ACHKUI_002205"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6598392.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            36546..37097
                     /gene="yfcE"
                     /locus_tag="ACHKUI_002206"
     CDS             36546..37097
                     /gene="yfcE"
                     /locus_tag="ACHKUI_002206"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6598393.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            37155..37697
                     /gene="yfcD"
                     /locus_tag="ACHKUI_002207"
     CDS             37155..37697
                     /gene="yfcD"
                     /locus_tag="ACHKUI_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6598394.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(37730..39250)
                     /gene="yfcC"
                     /locus_tag="ACHKUI_002208"
     CDS             complement(37730..39250)
                     /gene="yfcC"
                     /locus_tag="ACHKUI_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6598395.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
BASE COUNT         9367 a         9960 c        10685 g         9356 t
ORIGIN      
        1 gagaatactt gtttacattc tatgattata ttggagtgta tatgttcaat ggctaactat
       61 aagtattgga catgacatat attaatttta tgtcaggtgc ttatattata actcttaatg
      121 atttaaggac aaataatgag taagtttgcc aaaacagcca ttgctgcggc gatggtcatg
      181 ggcgcagttg tttccagttc tgcttttgca gcaggcaaca acggtaccgc gcgcttctac
      241 ggcaccattg aagactcccc gtgctctatc gttccagacg atcacaaact ggaagtcgat
      301 atgggtgaca tcggtgcggc taaactgacc ggcggtggca ctaccacacc gaaagatttc
      361 cagatccgtt tacaggattg cgtgtttacc actgaaacaa acatggagac cacgttcacc
      421 ggcactaact acgcggggcc agccaacgca gataactatg cgttattcaa tgttgacagc
      481 ggtgttgcca tgaaccacgt gagcctggta atcggcgata cccatggcaa aggttataaa
      541 gtgggcgaaa agattgttca gccaatcgtg atggatactt caaccagcaa gggtaaagac
      601 aagcaaacgc tgaactttaa agcctggctg gtgggtgaaa ccgatgcgcc ggatctcggc
      661 cagttcgaaa ccctgaccac attccagatt acctatctct gatgtgacaa ccccacagcg
      721 ttctgctgtg gggttattca acacgcaaca gatttacgac tgtttcacaa acggatatgt
      781 gtatgcctaa tcactcaaat tttcggctgc ggggaatcgc ctgctatatt gcgctggcaa
      841 tctctggtgg gtcagtcaat gcatgggctg atgattcaat tcaatttgac ccccgtttcc
      901 ttgagttaaa gggcgatacg aaaattgatc tcggtaagtt ttcaaaaaaa gggtatgtcg
      961 acgcgggtaa atataattta cgtgtattta taaataaaca acccctttct gatgaatacg
     1021 acattaactg gtacgtctct gaaaacgatc caacaaaaaa ctatgcctgc ctgacacctg
     1081 agttagtggc ggcgctgggg ctgaaagaag ggatagcaaa aagcctgcag tggacgcaca
     1141 acgatgaatg ccttaaacca ggccaattag acgggatgga agtcgagaat gatttaagcc
     1201 agtcggcgtt gctgctgaca gtgccacagg cttacctcga atataccagc agcgactggg
     1261 accctccctc acgctgggac gacggtattc ctggcctgat tgccgactac agcctcaatg
     1321 cgcaaactcg ccaccaggag caggggggcg aggactcaca tgatatcagc ggcaacggta
     1381 ccgttggggc gaacctgggg gcatggcgtt tccgtgcaga ctggcaaagt gattatcagc
     1441 acacccgcag caacgatgac gacgatgaca gcagtaacag cacaacgagt aaacactggg
     1501 actggagtcg ttattatgcc tggcgagcct tgccctcttt aaaagcgaag ctgtcgctgg
     1561 gggaagatta tctcaattcc gatattttcg acggctttaa ctatatcggc agcagcgtca
     1621 gcaccgatga ccaaatgctg ccgcccaacc tgcgcggcta tgcaccggat gtttccggcg
     1681 tggcgcacag cagtgcaaaa gtcaccatta gccagatggg ccgggtactt tacgaaaccc
     1741 aggttccagc cgggccattc cgcattcagg atatcggcga ctccgtctcc ggcacactgc
     1801 acgtccgcgt tgaagaacag aatggtcagg tgcaggaata tgacgttacg accgcatcta
     1861 tgccattcct cacgcgccag gggcaggtgc gttacaaagt gatgatgggg cgtccggaag
     1921 actggaacca caagaccgaa ggcggctttt tctccggcgg agaagcgtca tggggggtgg
     1981 cagatggctg gtcgctctac ggtggtgcgc tggcagataa acactatcag tcggcggcga
     2041 tgggggtggg gcgcgacctc gcacagtttg gcgcgctggc gttcgatgtg actcactcgc
     2101 acgtcaacct ggatcatgac agcgcatacg gcaaaggaaa actggacggc aactcctttc
     2161 gcgtgagcta tgccaaagac tttgacgaac tcaacagccg cgtcaccttt gcaggctacc
     2221 gtttttctga aaagaacttc atgaccatga gcgagtatct ggacgcgaac cagtcggaca
     2281 tggcgcggac cggtaacgac aaagagatgt atacgatcac ctataaccag aactttgccg
     2341 ctgcgggtgt ctcgatctat ctcaactact cccatcgtac ttactgggat cgcccggaac
     2401 agacaaacta taacttgatg ttttcccact attttaatat ggggagcatt cgcaacatga
     2461 gcatctcggt gaccggctat cgctacgaat atgacgataa tgcggataag gggatgtacc
     2521 tcagtatgag cattccctgg agcgacagca gcaccgtgac ctacaacggt tcctacggca
     2581 gcgggtcgga cagcagccag gtcggttact ttaagcgcgt cgatgacgca acgcactacc
     2641 aggttaacgt tggcaccagc gagcagcacg gcagcgtgga tggttatctg agccacgacg
     2701 gttcgctggc gaaggttgat ctcagcgcca actaccatga aggggaatac cgttcggcgg
     2761 ggatcgcctt acagggcggg gcaacgctga ccgcgcatgg tggggcgctg caccgtaccc
     2821 agaacatggg cggcacgcgc ctgctgattg acgccgacgg gattgccaac gtgccggtcg
     2881 aaagcaacgg cgcgccggtg tacaccaata tgtttggcaa ggcggtggtc gccgatatta
     2941 acaactacta tcgcaatcag gcgtatatcg atttaaacaa cttgccggaa gatgcggaag
     3001 ccacccagtc ggtggtgcag gcgactctga cggaaggcgc aatcggctat cgtaaattca
     3061 aagtgatcag cgggcaaaaa gcgatggcgg tactgcggtt gcgtgacggt agctacccgc
     3121 cgtttggcgc ggaagtgaaa aacgacgagc agcagcaggt tggcattgtg gatgatgaag
     3181 gcaatgtcta tctggcgggg gtcaatgcgg gcgagcatat gatggtgttc tgggaaggca
     3241 gcgcacaatg cgagatcgta ttgccgaagc cgctacctgc cgatttgttc agcggcctgt
     3301 tgttgccgtg tgaacaaaag ggaacggcag cccctgattc ttcagcgcca gaaattaagc
     3361 ctgttattca ggaccagacg cggcaagtca caccaacgga agcgccgacg tcaatttcag
     3421 caactcaata atctgattaa ggaacgatca atgtcttata aattatcctg tccaatgctc
     3481 gtcagcacag cgctgatggc actgcttacc accgccagcc tgacggcgca tgcatcggtc
     3541 accccggatc gcacccgtct ggtgtttaac gaaagcgata aatcgatcag cgtgacgttg
     3601 cgtaacaaca acgaaaagct gccttacctg gcgcaaagct ggctggaaga cgagaagggc
     3661 aacaaaatca cctcgccgct ggcggtactg ccgccggtgc agcgtattga cgccatgatg
     3721 aacggccagg tgaagattca ggcgctgccg gacattcact cgctgccgtc ggaccgcgaa
     3781 agcctgttct actataacgt gcgtgagatc ccgccaaaga ccgggaaagc caacaccctg
     3841 caaattgcgc tgcaaacgcg catcaagctg ttctggcgac caaaggcgct ggagaagatt
     3901 gacatgagaa aaccgtggca gtttaaggtc acactcacgc gcagcggcca ggactatacc
     3961 gtcaataacc cgacgccgta ccacgtcatc atcagcgatg ccagcacaca gaagaaaggg
     4021 ctgaccgccg ccgggtttaa accgctggtg atgccaccga aaacgtcaca accgttaaag
     4081 gcaaaaatgg cgagcgcccc ggtgctgacc tatatcaacg actacggcgc gcgcatgccg
     4141 ctgatcttcc gttgtgaagg gaacacctgc aaggtggatg aagatcaaag cagcaaaggt
     4201 tgataacggg agaagcaaca tgaaagcgtt ctttctgacc ggatctgccg cgctattgct
     4261 ggcctgtggc gtgaccgttt tcccggcaat ggcgggcggc aaaatcgtca atttgacgtt
     4321 gcgcgtgctg gtggatggac cgccgccctg ttcggtaaag ggaagcgtgg tggagttcgg
     4381 caatgtgatt attaaaagca ttgatggtgc caactaccgt cagccagtgg gctacaccct
     4441 gaactgctca aacagtgtca gcgacgacct gcaaatgcag cttcaggcca ccaccacgac
     4501 catcaacggc gaaacggtgc tgagtactgg aatcagcggt tttggcattc gtattcagaa
     4561 tgctgtcgat cattcgctgg tggcagtagg taacagtagc tggctgccgt tcaacatcaa
     4621 tactcagcca gacttcgaag ccgttccggt gaagcagagc ggtgtggaac tgatggcatc
     4681 ggagtttaac gcgaccatga ccatggtggt ggactaccaa tgaaacgact cgcatggtgc
     4741 ttaatttacg gttttgctgg cctggtgcag gccgcgatta acgacgttac ttttcacggc
     4801 acgctggttt cgccgcccgc ctgtactatc agcgatggta agacgatcga agttgagttt
     4861 cgcaacgtca ttatcgacaa cattaacggc gataatttcc gtcaggatgt gccttacacc
     4921 atcacatgtg accccgatgt gcgcgacgac gcctgggaga tgagcctgac ctggacgggc
     4981 agccaaaccc cttatgatga ttccgccatt gaaacggacg ttagcggcct ggggattgag
     5041 ctacagcaaa acggccagcc gtttaagctg ggaacaccgt tgaaaattga cccgtcaacg
     5101 ccgccaacgc tgcaagctgt gccggtgaaa gccaatgatg cggcgctcag cgacggaacc
     5161 ttttctgcct acgcaacatt acaggtggat taccaatgaa cctaaaacgg atatctcact
     5221 gcgcggctgc actggcgacg gtgctgctga cgccccatgt tcaggcggct gacaacctgc
     5281 atttctacgg caatttactc agcaagtcct gcacgttagt cgttcagggc gagtcgctgg
     5341 cggacgtgca tttccccacc gttagccgca aagacctgat ggtgaccggg cagtcggcgc
     5401 gggtgccggt agtgtttcag ttgaaagatt gtaaagggcc agcccagtat gaggttcggg
     5461 tgacgctgac cggtacagaa gacagtgaac agcccggctt tctggcgctg gatgcgtcct
     5521 caggggcgca gggcgtgggg attggcatgg aaaaaaccga tggaacaccg gtgccgatta
     5581 acaataccag cggcgcgacc tttgtgctga gtaacggcaa taacaatctg aattttaatg
     5641 cctggctcca ggcgaaaagc ggccgggaag tgacgatggg tgaatttacc gcttctttaa
     5701 ccgcaacgtt tgagtacatt taagatgaaa ataactaact tgctgtttcg tcttttgatc
     5761 ctgatggttt ttatgctcgg tagtcgggcc gggatggggc agacatttcg taatgtgtat
     5821 atctactatg ctacgtttgg ctggaatagt caggtcgaac tgtatcttga tgttgctgag
     5881 gtttcgagtg tttactacgg tacctattat caaagtaatt atgttatgac gcttggcaac
     5941 ttacccaatc gcacatggac aggaacgggc agttctcctg cgccgacgat tacggtaaaa
     6001 gatgggacaa ctgtaaataa ttcattttgt ccgggcatgg aggcactgga agccaaagac
     6061 agccgctggg aatgtctcaa gctgcctctg gatattaccg tcgatggtga tgtgggaggc
     6121 tgcccgtggt tagtgagtat cagagccacc agccaggttg tggtcggttc tgttgataaa
     6181 gatatctatt atggaccaaa tgcaaccgtg tccgtgtgcc caaccgtgtc agttacgccg
     6241 tatgatgttt catgggatga aaactatatc aataaaacga aaacactgtc tttgcaatcg
     6301 acgggcggag taattgaaaa aacgctttca acctatctga tgaaagacgg caaactatgt
     6361 gatggctcgc aaatgaatga agagggcgct tactgtcgct gggtcgcgca aatgatcacg
     6421 ttttcatcgt caggctgcga taatgctaat gttacggtga cgccaaacag acatcccatt
     6481 actgacaaac aactgcacga catggtggtg catgttgata ccagcagccg ccagcccatt
     6541 gattccacct gccgcttcca gtacacgctc aacgagctgt agcgtataca ccacacaacg
     6601 gggccatcag gccccgttgt ccgttatgtc atgcggtcat ccagtttttt agaatattcg
     6661 ccatcacggg agcgaaaggt gccagataac cgtcgacgag ggtgagtaat tcgtcgataa
     6721 gcgcttgcag caggtcgtca tcctcaagcc cctctgaagg ggcaatgcca ttagcccaca
     6781 gaaggctgtc ggaaagctgc ttacgttttt caattaatgc ccgattatta cgttcattat
     6841 cgccagggtt gagacgccag tattcaaggc taaatggaca gactcgtcca cctttaaata
     6901 ttcagggcaa ttccggcggc agccactcat ctacttctat caacaccagc aatgccgcat
     6961 caccatcata ctcttttggt gcctggtgaa atgccattac atggggatgt tgcgccagcc
     7021 acagtggcgt ttgctgcttt aaaatatgct taccatgacc atgcatcaca caggcgcaaa
     7081 acacatgttc acggcggcag gcggcaatca acgcccccag ttcctgtttg gcttgtagct
     7141 gcgtcagacc gtgcaaatca agaaacaact ccggggaata atcaccgcgc cgcagtttct
     7201 tcgcttcaaa atggctgaca tccgggcgaa catatttcac cggaccttca gtatttaata
     7261 atggttgaaa ctcgtcagag aaataatggc tggcatcggc ctgctcctga atcaaccgct
     7321 tgaccggtac ttcgctgatt ttcttgcgtt gtggtcgatg gacaatcgtg tcctgcttaa
     7381 tcttgcgagt ccccgccatc aactggcgaa acagcgcctg gtcctcctcg ctaagtgttg
     7441 ttttcttttt catttaactg tctctatttc taattttcct acagtttacc cgactcgtag
     7501 cacaatcgat cgttttaaac gtatttttcc tcactcaccg cgcgtgaatg ctgatttatc
     7561 gtcatcttca tggcaaacta gccgccgaaa ttattgcgat catgccctgg aggaatacgt
     7621 ggataaaatt ttcgttgatg aagcagtaaa tgagctgcaa accattcagg acatgttgcg
     7681 ctggtcggtg agccgcttca gcgcggcgaa tatctggtac ggccacggca ccgataaccc
     7741 gtgggatgaa gccgtgcaac tggtgttgcc ttcgctctac ctgccgctgg atattccgga
     7801 agacatgcgc accgcgcgtc tgacctccag cgaaaaacac cgtattgttg agcgcgtgat
     7861 ccgccgcgtc aacgagcgca ttccggtggc ttacctgacc aacaaagcct ggttctgcgg
     7921 ccatgaattt tacgtcgatg aacgcgtgct ggtgccgcgc tcgccgattg gtgaactgat
     7981 caacaataaa tttgccggac tcatcagcaa gcaaccgcag catattttag atatgtgtac
     8041 cggtagcggc tgcatcgcca ttgcctgtgc ttatgccttc ccggaagcgg aagtcgacgc
     8101 ggtggacatc tctccagacg cgctggcggt tgctgaacag aacatcgaag aacacggttt
     8161 gatccacaac gtcattccga ttcgttccga cttgttccgt gacttgccga aagtgcagta
     8221 cgacctgatc gtcaccaacc cgccgtacgt cgatgcagaa gatatgtccg acctgccaaa
     8281 cgaataccgc cacgagccgg aactgggtct ggcatctggc accgacggcc tgaaactgac
     8341 gcgtcgcatt ctcggtaacg cggcagatta cctcgctgat gatggcgtgc tgatttgtga
     8401 agtcggcaac agcatggtac atcttatgga acaatatccg gatgttccgt tcacctggct
     8461 ggagtttgat aacggcggcg atggtgtgtt tatgctcacc aaagagcagc tgctggcggt
     8521 ccgtgaacat ttcgcgattt ataaagatta agtaaacacg caaacacaac aataacggag
     8581 ccgtgatggc tggaaacaca attggacaac tctttcgcgt aaccaccttc ggcgaatcgc
     8641 acgggctggc gctcggctgc atcgtcgatg gtgttccgcc aggcattccg ctgacggaag
     8701 cggacctgca acatgacctt gatcgtcgtc gccctgggac atcgcgctat accacccaac
     8761 gccgcgagtc ggatcaggtc aaaattctct ccggtgtttt tgaaggtgtt actactggca
     8821 ccagcattgg cttgttgatc gaaaataccg accagcgttc tcaggattat agtgcgatta
     8881 aggacgtttt ccgtccaggt catgccgatt acacctatga acaaaaatac ggcctgcgcg
     8941 attatcgcgg cggtggacgt tcttccgccc gtgaaaccgc tatgcgcgtg gcggcagggg
     9001 cgattgccaa aaaatatctc gccgagaaat ttggcattga gattcgcggt tgcctgaccc
     9061 agatgggcga cattccactg gaaatcaaag actggtctca ggtcgagcaa aatccgtttt
     9121 tctgcccgga cccggacaaa atcgacgcct tagatgaact gatgcgcgcg ctgaaaaaag
     9181 agggcgactc catcggcgcg aaagtcaccg tcgttgccag tggcgtaccc gccggacttg
     9241 gcgagccggt ctttgatcgc ctggatgccg acatcgccca tgcgctaatg agcattaacg
     9301 cggtgaaagg tgtggaaatt ggtgatggtt ttgacgtggt agcgctgcgt ggcagccaga
     9361 accgcgacga aatcaccaaa gacggtttcc agagcaacca tgcgggcggc attcttggcg
     9421 gtatcagcag cgggcagcaa atcattgccc atatggcgct gaagccaacc tccagcatta
     9481 ccgtgccggg gcgcaccatt aaccgctttg gcgaagaagt tgagatgatc accaaaggtc
     9541 gtcacgatcc ttgtgttggg atccgcgcgg tgccgatcgc ggaagcgatg ctggcgatcg
     9601 ttttaatgga tcacctgtta cggcaacggg cgcaaaatgc tgatgtgaag actgatattc
     9661 cacgctggta aaaaatgaac aaaaccgcga ttgcgctgct ggccctgctt gccagtagcg
     9721 ccagcctggc agcgacgccg tggcaaaaaa taacccaacc tgtgccgggt agcgcacaat
     9781 cgataggcag tttttctaat ggctgtatcg tcggtgctga cacgttgccg atacagtccg
     9841 aacattatca ggtcatgcgt accgatcagc gtcgctattt cggacatccg gatctggtga
     9901 tgtttatcca gcgtctgagt cgccaggtga gcaatctggg catgggtacg gtgctgattg
     9961 gcgatatggg gatgcctgct ggtggtcgtt tcaacggcgg tcacgccagc caccagaccg
    10021 gactggatgt cgatatcttc ctgcaactgc caaaaacacg ctggacctcc gcgcagctct
    10081 tgcgcccgca agcactggac ttagtatccc gcgacggtaa acacgttgtc cccgcgctgt
    10141 ggaagccaga aattttcagc ttgatcaaac tcgccgccca ggacaaagac gtcacgcgca
    10201 tttttgttaa tccggcgatt aaacaacaac tttgccttga tgcgggcacc gatcgcgact
    10261 ggttgcgcaa agtgcgaccc tggttccagc atcgcgcgca tatgcatgta cgattacgtt
    10321 gtcctgccga tagtctggag tgtgaggatc aacctttacc gccaccaggc gatggttgcg
    10381 gggcagaact gcaaagctgg tttgaacctc caaaaccggg aacaacaaag cctgagaaga
    10441 agacaccgcc tccgttgccg ccttcctgcc aggcgctact ggatgagcac gtgatctaat
    10501 ggaaacgttt aatagcctgt ttatggtttc cccgctgctg ctgggagttc tcttttttgt
    10561 cgccatgctg gcggggttta tcgactcgat tgccggtggc ggtgggttac tcaccattcc
    10621 ggcattgatg gcggcgggga tgtctcccgc taacgcgctg gcaaccaata aactgcaagc
    10681 ctgcggtggc tctatttccg ccactatcta ctttattcgc cgtaaagtgg ttagtttaag
    10741 cgatcaaaaa ctcaatatcg ccatgacctt tgtcggctca atgagcggcg cgctactggt
    10801 gcagtatgtt caggccgatg tcttacggca gattttgcct gtcctggtca tttgtatcgg
    10861 tctctatttc ttgctgatgc caaagctggg ggaagaagat cgccagcgtc ggatgtatgg
    10921 actgccattt gcgctggtgg cggggggctg cgtcggtttt tacgatggat tttttggccc
    10981 ggctgccgga tcgttttacg cgctggcctt cgttacgctg tgcggattca acctcgccaa
    11041 agccactgct cacgccaaat tacttaacgc aacgtcaaac attggcggtc tgctgctgtt
    11101 tattctcggc ggcaaagtga tttgggcgac gggctttgtg atgctggtgg ggcagttcct
    11161 cggcgcgcgc atgggttcaa gactggtgtt gagcaaagga caaaagctga tccgcccgat
    11221 gatcgttatc gtctcggcgg tgatgagtgc caaattactt tacgatagcc atggacagga
    11281 gatcctccac tggttgggga tgaactaatg aacagtacac accactatga gcagttgatt
    11341 gagattttta atagctgctt tgccgatgaa tttaataccc gtctgattaa aggcgacgac
    11401 gaaccgatct atcttcctgc tgatgcggaa gtaccgtata acagaatcgt cttcgctcat
    11461 ggcttttatg ccagcgccat ccacgagatt tcgcactggt gtattgccgg gaaagcgcgc
    11521 cgtgagctgg ttgatttcgg ctactggtat tgcccggacg gacgcgatgc ccagacacaa
    11581 agccagtttg aagatgttga agtgaagccg caggcgctgg actggttgtt ctgcgtggca
    11641 gcgggatatc cgtttaatgt tagctgcgac aatctggaag gggatttcga accggaccgc
    11701 gtggtgtttc aacggcgggt acatgcgcag gtgatggatt atctggcaaa cggcattccc
    11761 gaacgtccgg cacgcttcat taaagcacta caaaattatt atcacacgcc ggaacttacg
    11821 gcggaacagt tcccgtggcc ggaagcgctc aactgaagcc agcgccacgt tttacaatga
    11881 ggaaagaaga tgatcgcgga gtttgaatca cgcattctgg cattaatcga cggtatggtt
    11941 gaccatgcca gtgatgatga gttgtttgcc agtgggtatt tgcgtggcca cctgacatta
    12001 gccatcgcag aactggaaag tggtgacgac cactccgctc aggcggtaca tacaaccgtt
    12061 agccagagtc tggaaaaagc cattggcgcg ggtgaattgt caccgcgtga ccaggcgctg
    12121 gtaaccgata tgtgggaaca cctgtttcag caggcctcac agcagtaagt tgtgacgtct
    12181 ggagcaccat gcctgatgcg acgcttatcg cgtcttatca ggcctacagg taacgcgtag
    12241 gtcggataag gcgtttacgc cgcatccgac aaacaatgcc aaagcagatt accccgcctt
    12301 aaccgcttta cctttcaaca acttccgcac ccataaccta ttcggattta acgccgccag
    12361 cgtactggca tccatcggaa tcggttcttc actcatctgt gctgccagaa tctcggcaca
    12421 cagcggggca gaacacaaac cgcgcgagcc caaagcagca agcataaaga gatcgtcata
    12481 aaccggcgcg cttaccgctt catctttctt ctccgccagc gacgcatatt ccacgagtgt
    12541 agcgtcataa tcaggcacgt tgcctaccat tggcagatga tcgcgagtag cacaacgcac
    12601 accgcagcgc gcctctttac cactgacatc aacctctttt gcccactgta cctgcgggaa
    12661 acaatcaatc aaccgctggc gattctgctg ctgatcttca tcactgtacg ccgtctcttc
    12721 gctaccacgg tgataactgg caccaataca atgatgctga ttcgccggat tttgcggcgt
    12781 gagataaccg tcatagcaca gcacttgctt cagtttcgcc agctccggcg ttgtcggaat
    12841 atggctgacc tgcccggcaa ccgaatatac tggcagagat gacgtttggc tgaaacggct
    12901 gatttgatgc ccgttcgcca gtaccactac actgtgtgtt gcttgctgat ctcctgcaaa
    12961 attcaacaac caacagtcat ccttacggga taaatcctgt aactgatgtt gataatgaat
    13021 ctgcaaaccc tgctgttgcg ccagtttcag cacattacgg gtcagttctg ccggacacag
    13081 ccagccacct tgcggataag taataccgct gcaatttgtc gcaacgcccg taatttgttc
    13141 aaccgcattt gcctcaacgg ctacagccag ttctgcgggt aaatccatcg acaacatctg
    13201 tgcgatttta tgctggcttt tctcatccca gcctaactgc gtgacgccgc accagtcatg
    13261 atcaaattta acgggcaata aatcgtaaaa ccgacgagca aaagtaaacg cattagagaa
    13321 aaaacggttt agcgcttcat cgtgtttact taaaagcgga tacagcgccc cctggcgatt
    13381 gccggaagca cccagtgcgg gagcctcatc cgcgcaataa agcgttacct gccagccgcg
    13441 acgcaatagc gccagcgaca acaacgcgct ggcaataccg cctccaataa ttgccgcttc
    13501 ttgtttgttg ctgcccgtac ggttaaacca cggcgtggag caggggaggg gtaatgtctg
    13561 ttccatcacc ccacaaagca tttcccgttt gcgcccaaag cccttacgtt tttgcatagt
    13621 gaatccggct tcctgcaaac cgcggcggac aaaaccggca gacgtaaatg tcgccagcgt
    13681 gccgcccgga cgtgccagtc gcgccatggc gttaaacaga ttttgtgtcc acatatccgg
    13741 gtttttcgct ggcgcaaagc cgtccagaaa ccaggcatct actttttgat ttagcgaatc
    13801 gtccagtttg ctggtcagtt cattaatatc gccaaaccat aaatccagcg tcacgcggcc
    13861 ttcatcgagc aataaacgat ggcaaccggg caagggcaaa ggccattgcg cctgaagctg
    13921 ttccgcccac ggggccagct ccggccagtg ttgatgcgcc agcgctaaat ccgcacgggt
    13981 gaggggaaat ttctcaaaac taatgaaatg taagcgttgt aattgcgctt gcggatgtgc
    14041 ttcgcgaaac tgatcaaatg cctgccatag cgtcaggaag tttaatccgg tgccgaagcc
    14101 gctctctgct accacaaaca gaggatgtgg atgttcagga aaacgtgcct ctaaatggtt
    14161 acccccgaga aaaacataac gcgtctcttc cagtccgtta tcgttggaaa aatagacatc
    14221 gtcaaaatct cgggaaacag gtgtaccctc agcattaaat tcgaggttgg caggttgtat
    14281 ggcgtagtgt ttcacgtaag ttactcgtct tacaggcggt ggctcgatct tagcgatgtg
    14341 tgtcaggctg cgcaaatttc tctattaatt ggctgatcgg acttgttcgg cgtacaagtg
    14401 tacgctattg tgcattcgaa acttactcta tgtgcgactt acagaggtat tgaatgaaac
    14461 gtgcagtgat tactggcctg ggcattgttt ccagcatcgg taataaccag caggaagtcc
    14521 tggcatctct gcgtgaagga cgttcaggga tcactttctc tcaggagctg aaggattccg
    14581 gcatgcgtag ccacgtctgg ggcaacgtaa aactggatac cactggcctc attgaccgca
    14641 aagttgtgcg ctttatgagc gacgcatcca tttatgcatt cctttctatg gagcaggcaa
    14701 ttgcagatgc tggcctctcc ccggaagctt accagaataa cccgcgcgtt ggcctgattg
    14761 caggttccgg cggcggttcc ccgcgttttc aggtgttcgg cgctgacgca atgcgcggcc
    14821 cgcgcggcct gaaagcggtt ggcccgtatg tggtcaccaa agcgatggca tccggcgttt
    14881 ctgcctgcct cgccacgccg tttaaaattc acggcgttaa ctactccatc agctccgcgt
    14941 gtgcgacttc cgcacactgt atcggtaacg cagtagagca gatccaactg ggcaaacagg
    15001 acatcgtgtt tgctggcggc ggcgaagagc tgtgctggga aatggcttgc gaattcgacg
    15061 cgatgggtgc gctgtctact aaatacaacg acaccccgga aaaagcctcc cgtacttacg
    15121 acgctcaccg tgacggtttc gttatcgctg gcggtggcgg tatggtagtg gttgaagagc
    15181 tggaacacgc gctggcgcgt ggtgctcaca tctatgctga aatcgttggc tacggcgcaa
    15241 cctctgacgg cgcagacatg gttgctccgt ctggcgaagg tgcagtgcgc tgcatgaaga
    15301 tggcgatgca cggtgttgat accccaatcg actacctgaa ctctcatggt acttcgactc
    15361 cggttggcga cgtgaaagaa ctggcagcta tccgtgaagt gttcggcgat aagagcccgg
    15421 cgatttctgc aaccaaagca atgaccggtc actcactggg cgctgctggc gtacaggaag
    15481 ctatctactc tctgctgatg ctggaacacg gttttatcgc cccgagcatc aacattgaag
    15541 agctggacga gcaggctgcg ggtctgaaca tcgtgaccga aacgaccgat cgcgaactga
    15601 ccacagttat gtctaacagc ttcggcttcg gcggcaccaa cgccacgctg gtaatgcgca
    15661 agctgaaaga ttaattcgga gtaggtcgga taagacgcgc cagcgccgca tccgacgtta
    15721 cgcgccaatg ctgcctccgg cactaacgca aaagggaacc cgatggttcc ctttttcaca
    15781 tcattgacaa tcgccgccag ttccaggcaa acttcccgct ttgtcgattt cctgcagaaa
    15841 agacgtacgc gttaaatcct gccaacgcac cgtaaccctg aaaccagaga gatgagacgg
    15901 ggatactcct cgccttgcgc tgcattctgg agtaatgcat gactgctgta agccaaaccg
    15961 aaacacgatc ttctgccaat ttttcgctct tccgcatcgc ttttgcggtt tttctcacct
    16021 acatgaccgt aggtttaccg ttgccggtta tcccgctgtt tgttcatcat gaactgggct
    16081 atggcaacac tatggtcggc attgccgtcg ggattcagtt tctggctacg gtgctgacgc
    16141 gtggttacgc cgggcgactg gccgatcaat atggcgcaaa acgttcggcg cttcagggga
    16201 tgttagcttg tggtctggcg gggggcgcgt tgctgctggc ggcgattttg cccgtccccg
    16261 caccgttcaa atttgctctg ttggtcgtcg ggcgtttgat tcttggcttt ggtgaaagcc
    16321 agttactgac gggcgctctg acctggggat taggcatcgt tgggccaaaa cattccggca
    16381 aagtgatgtc atggaacgga atggctattt acggtgccct cgctgttggt gcgccgcttg
    16441 gcctgttgat tcatagccat tacggttttg ccgcactggc gctcaccaca atggcattac
    16501 ccttactggc gtgggcctgt aacggcacag tgcgcaaagt accggccctg gcgggggaac
    16561 gtccatcgct gtggagcgtt gtcgggctta tctggaaacc agggttaggt ctggcactac
    16621 aaggcgttgg ttttgcggtt atcgggactt tcgtttcgct ctactttgcc agcaaaggat
    16681 gggcgatggc gggctttact cttaccgcgt ttggcggcgc atttgtcgtg atgcgcgtca
    16741 tgtttggctg gatgccggac cgttttggcg gcgtgaaagt ggcgatcgtc tctctgcttg
    16801 tagaaacggt gggcttgttg ctgctctggc aagccccagg ggcgtgggtc gcattagcgg
    16861 gcgcggcgtt aactggagcc ggatgttcgc ttatctttcc tgcgctgggc gtggaggtgg
    16921 ttaaacgcgt cccctcacac gttcgcggca ccgcactggg cggttacgcc gcgtttcagg
    16981 atatcgccct cggcgtctcc gggccgcttg cgggaatgct ggcgaccatg tttggttact
    17041 cttcggtatt tcttgccggg gcgatctctg cggtgctggg aattattgtc acgatactgt
    17101 cgtttcgtcg gggttaacga accagccaga ccagcatcag gataatcgcg actaacagta
    17161 tccacagcgc gggacgcgtt gccatatttt gcaacgtgtc catcatcggt ggaaaaggat
    17221 taagcagcgg ttgcatatca cgcggctcaa tccctttaac ccgccgttga taaagctggt
    17281 taagaatatc ctcggcctgg gcagaagaaa gcgatgattg cgcagaaagg ccatattttt
    17341 gctggatata tgccgataac gccgccagtt cactggcatc taaaggttgt ttcagcgcca
    17401 tctgtagtga ttcaagcgtc ggcgtatttt gctggcttag cgtctgacgc gcctgtagcc
    17461 aggtcaccag atggttaaac agtttcgctg gaattaactc gccatctttc accccggaaa
    17521 gttccagcat cgattgccag atctgtttgc tgggttcccc cgttgctgcc gcaagtttgg
    17581 tcaccagctg tttgagcgca ttgtgctccg ccggtaataa aggacggtcg gtcgcctcgc
    17641 gctgctgtgg ttgcggaata accatcttcc cttcctgcaa cagggtgaga atggttttta
    17701 attgctccgg tgagagctga ttcagcggcg tctgaccaaa gttatgacgg atataatccg
    17761 tgaccgcctg acgattattc cccagacgta aatactcccc taattgcgct aaaagctggc
    17821 gggcagaatg gcttttctgc gcggccaaca gacgttgcgc cagattatgc tcagcggcag
    17881 ggaagtgacg cgaaagcagc ggtgaatctc ccgacagacc aatatcgtgc ctgatacccg
    17941 cccacagttc tgctctttgt tgttgcgtca gtgaggtcac tttcgtcatt aagctttcca
    18001 gcgaagtacg ttgctgactg gataaaggct gattgcccgc gccagacggc aggttatctc
    18061 cctgacctgg tggttgccca ggaggagggc cggaaatagg ttgtatcatt acgtatcctt
    18121 atacctgaaa tcttcgcaag tatgcctggc cgcgagatta tggcacactt gtctggttaa
    18181 ctctcgtctc atacaggtaa cacaaacgtg aaaatccttg ttgatgaaaa tatgccttat
    18241 gcccgcgact tatttagccg tttgggtgag gtgaccgcgg ttcccgggcg tccaattccc
    18301 gtcgctcaac tggcagacgc ggatgcgctt atggtgcgtt cggtcacgaa agtgaatgaa
    18361 tctttgctgg caggaaaacc cattaaattt gttggcactg ccaccgcggg gaccgaccat
    18421 gtcgatgaag catggttaaa gcaggcggga attggttttt ccgctgcacc tggctgtaat
    18481 gcgattgcgg tagtggaata tgttttctcc tccctgctga tgcttgccga acgcgatgga
    18541 ttttcactgc acgagcgtac cgtggggatc gttggcgttg gtaacgttgg tcgtcgttta
    18601 caggcgcgac tggaagcgtt agggattaca accttgcttt gcgatccgcc tcgcgccgac
    18661 cgtggggatg agggggattt ccgctcgctg aatgagttag tccagcatgc ggatattctg
    18721 actttccata cgccactctt taaagatggt ccgtacaaaa cgctgcatct ggcggatgaa
    18781 aaactgatcc gcagcctgaa accgggagcg attctgatta acgcctgccg tggcgcagtc
    18841 gtcgataata ccgccctgct gacctgcctg aacgaaggcc agaagttaag cgtagtgctg
    18901 gatgtctggg aaggcgaacc ggaacttaac gtggaattgc tgaaaaaagt ggatatcggc
    18961 acgccgcata tcgcaggcta taccctggaa ggtaaagcac gcggtactac gcaagtgttt
    19021 gaagcttata gcaagtttat tgggcatgaa cagcacgttg cactggatac attactgccc
    19081 gcgccagagt ttggtcgcat tacgctgcat ggcccgctcg atcagccaac gctgaaaagg
    19141 ctggtgcatt tggtgtatga tgtgcgccgc gatgacgcac cgctgcgtaa agtcgccggg
    19201 ataccgggtg agttcgataa actgcgcaaa aactatcttg agcgccgtga atggtcatct
    19261 ctgtatgtaa tttgtgatga cgccagtgcg gcatcattgc tgtgtaaact gggttttaac
    19321 gccgttcatc atccggcacg ttaatctctt cttcatgctc tctgctgtaa cattggcagg
    19381 gagctttgct atttctggag taaaccacca tgtctgaagg ctggaacatt gccgtcctgg
    19441 gcgcaactgg cgctgtgggc gaagccctgc ttgaaacgct ggctgaacgt cagttcccgg
    19501 ttggggaaat ttatgcactg gcacgtaacg aaagcgcagg cgaacaactg cgctttggtg
    19561 gtaagacaat caccgtgcag gatgccgctg aattcgactg gacgcaggcg cagctggcat
    19621 tttttgtcgc aggcaaagaa gctaccgctg cctgggttga agaagcgacc aactcaggtt
    19681 gtctggtgat cgacagcagc ggattgtttg ctctcgaacc cgacgtaccg ctggtggtgc
    19741 cggaagtaaa cccgtttata ctgaccgatt accgcaaccg gaatgtcatc gccgtaccag
    19801 acagtctgac cagccagctg ctggcggcac tgaaaccgct aattgatcag ggcggtttat
    19861 cacgtatcag cgttaccagc ctgatttcag cctccgccca gggtaaaaaa gcggtcgatg
    19921 cattagcggg gcagagtgct aaattactca atggcattcc gattgacgaa gaagatttct
    19981 tcgggcgtca gctggcgttc aacatgctgc cgttactgcc ggatagcgaa ggtagcgtgc
    20041 gtgaagaacg tcgtatcgtt gacgaagtac gcaaaatcct gcaggacgaa gggctgatga
    20101 tttcggcaag cgtcgtccag gcaccggtat tctacggtca tgcccagatg gtcaactttg
    20161 aagcactgcg tccgctggcg gcagaagaag cgcgtgatgc gtttgctcag ggcgaagata
    20221 ttgtgctctc tgaagagaac gaattcccga ctcaggtagg agatgcttct ggtagtccgc
    20281 atctttctgt tggctgcgta cgtaatgact acggtatgcc ggagcaagtc cagttctggt
    20341 cggtggccga taacgttcgc tttggcggcg cgctgatggc agtaaaaatc gccgagaaac
    20401 tggtgcagga gtatctgtac taatgtccga ccagcaacaa ccgccagttt ataaaattgc
    20461 gctgggcatt gagtacgacg gcagtaggta ttacggctgg caacggcaga atgaagtccg
    20521 cagtgtgcag gagaagctgg aaaaggcgct ctcccaggtg gcgaacgaac ccatcaccgt
    20581 cttctgcgcc gggcgtactg acgcaggggt acacggtacc gggcaggtgg tgcatttcga
    20641 aacgaccgcg cagcgcaaag acgctgcgtg gacgttaggc gtaaatgcga atttacctgg
    20701 tgacatcgcc gtgcgttggg ttaaagctgt acctgatgat tttcatgccc gatttagcgc
    20761 cacggctcgc cgttatcgct acatcatcta caatcatcgg ctgcgcccgg cggtattgag
    20821 taaaggggta acccattttt acgaaccgct ggacgctgaa cggatgcatc gggctgcgca
    20881 atgtttgctg ggcgagaatg atttcacctc gttccgtgcg gtgcagtgtc agtcccggac
    20941 cccgtggcgc aacgttatgc atattaatgt cacgcgtcac ggtccttatg tggtggtaga
    21001 tatcaaagcg aatgcctttg tacatcatat ggtcaggaat attgtcggca gcctgatgga
    21061 agtcggtgcc cacaaccagc cggagagctg gatagcagag ttgctggcgg caaaggacag
    21121 aacgctggcg gcagcaacgg caaaagcgga agggctatat ctggtcgcgg tggattaccc
    21181 tgaccgatat gatcttccaa aaccgccaat gggcccgcta tttctggcgg actaacgaga
    21241 ataatgctcg gaacatttca ggctgtagcc aacgtagtta cagcctgata gatgacgagt
    21301 acaaggcata ggcaaatatg gacctgattt atttcctcat cgattttatc ctgcacattg
    21361 acgtgcatct ggcagagctg gtcgcggagt acggcgtctg ggtttatgcc attttgttct
    21421 taattttgtt ctgtgaaacc ggtctggtgg taacgccgtt tttaccgggt gattcattgc
    21481 tgtttgttgc cggagcgttg gcatcactgg agaccaacga tctcaacgtc catatgatgg
    21541 tggtgctgat gctgatcgcc gcgattgtgg gtgacgcggt caattacact atcgggcggt
    21601 tgttcggtga aaagttattc agtaatccca attcgaaaat tttccgtcgc agttatctcg
    21661 acaaaaccca tcagttttat gagaaacatg gcggcaaaac gattattctc gcccgttttg
    21721 tgccgatcgt cagaacgttc gccccgtttg ttgcgggaat gggccacatg tcgtaccgtc
    21781 atttcgccgc ttataacgtg attggcgcac tgttgtgggt actgcttttt acctacgcag
    21841 gctatttctt cggcacgctc cccttcattc agagcaacct taaactgatg attgttggca
    21901 ttatttttgt ttcaatattg ccaggtgttt tcgaatttat tcgccacaaa cgcgcagcgg
    21961 cacgcgccgc aaaataaaaa gtaactctgc ggttcgacca cttttttatc caaagtttcg
    22021 ggctgttatg ttttaatgtg caacattcat ggtctgttgg gggcaaaaat ggcattatgc
    22081 gtccccaaag ataaaactgg catcgaacca ggttcagaca gaaaggtccc taatgagctg
    22141 gattgaacga attaaaagca acattactcc cacccgcaag gcgagcattc ctgaaggggt
    22201 gtggactaag tgtgatagct gcggtcaggt tttataccgc gctgagctgg aacgtaatct
    22261 tgaggtctgt ccgaagtgtg accatcacat gcgtatgaca gcgcgtaatc gcctgcatag
    22321 cctgttagat gaaggaagcc ttgtggagct gggtagcgag cttgagccga aagatgtgct
    22381 gaagtttcgt gactccaaga agtataaaga ccgtctggca tctgcgcaga aagaaaccgg
    22441 cgaaaaagat gcgctggtgg tgatgaaagg cactctgtat ggaatgccgg ttgtcgctgc
    22501 ggcattcgag ttcgccttta tgggcggttc catgggatct gtggtaggtg cgcgtttcgt
    22561 gcgtgccgtt gagcaggcgc tggaagataa ctgcccactg atctgcttct ccgcctctgg
    22621 tggcgcacgt atgcaggaag cactgatgtc gctgatgcag atggcgaaaa cctctgcggc
    22681 actggcaaaa atgcaggagc gcggcttgcc gtacatctcc gtgctgaccg acccgaccat
    22741 gggcggtgtt tctgcaagtt tcgccatgct gggcgatctc aacatcgctg aaccgaaagc
    22801 gttaattggc tttgccggtc cgcgtgttat cgaacagacc gttcgcgaaa aactgccgcc
    22861 aggattccag cgcagtgaat tcctgatcga gaaaggcgcg atcgacatga tcgtccgtcg
    22921 tccggaaatg cgcctgaaac tggcgagcat tctggcgaag ttgatgaatc tgccagcgcc
    22981 gaatcctgaa gcgccgcgtg aaggcgtagt ggtgccaccg gtaccggatc aggaacctga
    23041 ggcctgataa ctgataaggg cagggccact ggctctgccc ttttgctatt ctcaccgtaa
    23101 cgaatcagcg gataccatga ttatcaaacg cactcctcaa gccgcgtcgc ctctggcttc
    23161 gtggctttct tatctggaaa acctgcacag taaaactatc gatctcggcc ttgagcgcgt
    23221 gagccaggtc gcggcgcgtc ttggcgttct gaaaccagcg ccatttgtgt ttaccgttgc
    23281 gggtacgaat ggcaaaggca ccacctgccg tacgctggag tcgattctga tggcggcagg
    23341 gtacaaagtg ggcgtctaca gttcgccgca tctggtgcgt tataccgagc gcgtacgtgt
    23401 gcagggtcag gaattgccgg aatcggccca caccgcttct tttgcggaga ttgaatcggc
    23461 acgcggtgat atttccctga cctatttcga atacggtacg ctgtcggcgt tgtggctgtt
    23521 caagcaggca caacttgacg tagtgattct ggaagtaggg ctgggcggtc gtctggacgc
    23581 aaccaatatt gtcgatgccg atgtcgcggt agtaaccagt attgcgctgg atcataccga
    23641 ctggctgggg ccagatcgtg aaagtattgg tcgcgagaaa gcaggtatct ttcgcagcga
    23701 aaaaccggca attgtcggtg agccggagat gccttctacc attgctgatg tggcgcagga
    23761 aaaaggtgca ctgctacaac gtcggggcgt tgagtggaac tattccgtca ccgatcatga
    23821 ctggacgttt agcgatgctc acggcacgct ggaaaatctg ccgttgccgc ttgtcccgca
    23881 accgaatgcc gcaacggcgc tggcggcact gcgtgccagc gggctggagg tcagtgaaaa
    23941 tgccattcgc gacgggattg ccagcgcaat tttgccggga cgtttccaga ttgtgagcga
    24001 gtcgccacgc gttatttttg atgtcgcgca taatccacat gcggcggaat atctcaccgg
    24061 gcgtatgaaa gcgctaccga aaaacgggcg cgtgctggcg gttatcggta tgctacatga
    24121 taaagatatt gccggaactc tggcctggtt gaaaagcgtg gttgatgact ggtattgtgc
    24181 gccactggaa gggccgcgcg gtgccacggc agaacaactg cttgagcatt tgggtaacgg
    24241 caaatcattt gatagcgttg cgcaggcatg ggatgccgca atggcggacg ctaaagcgga
    24301 agacaccgtg ctggtgtgtg gttcgttcca cacggtcgca catgtcatgg aaatgattga
    24361 cgcgaggaga agcggtggca agtaagtttc agaatcggtt agtgggcacg atcgtgctgg
    24421 tagcgctggg ggtgattgta cttccagggc tgctggacgg gcagaaaaaa cattatcagg
    24481 atgagttcgc ggctatcccg ctggtgccaa aagcgggcga tcgcgatgag cctgatatga
    24541 tgccagccgc cacccaggcg ttgccgacgc agccgccgga aggcgcagcg gaagaggtac
    24601 gggcaggtga tgccgcagca ccgtcgctcg atccggccac tattgcagcc aataacaccg
    24661 agtttgaacc ggaacctgca ccggtcgccc caccgaagcc gaaaccggtg gagccgctta
    24721 agccaaaggt tgaagcacca cctgcgccga agccagaacc gaagccggtc gtggaagaaa
    24781 aagcagcgcc aacgggtaaa gcttatgttg tgcaactggg cgcgctgaaa aatgccgata
    24841 aagtgaatga gattgtcggt aagctgcgcg gtgccggtta tcgggtttat acgtcgccat
    24901 ccacgccagt gcagggtaaa attacccgta ttctggttgg gccggatgct tccaaagaga
    24961 agctgaaagg atcgctgggt gagctcaagc agctttctgg cttaagtggc gtggtaatgg
    25021 gctatacacc gaattaatac ggtcttgcct gatgcgacgc tggcgcatct tatcaggcct
    25081 gtgcaggggt agaaccgtag gtcggataag gcgttcacgc cgcatccgac agtcgttgcc
    25141 tgatgcgaca ctggcgtgtc ttatcaggcc tacgcagggg tagaacagta ggtcggataa
    25201 ggcgttcacg ctgcatccga cagccgttgc ctgatgcgac gctagcgcgt cttatcaggc
    25261 ctacgcagga gtagaacagt aggtcggata aggcgttcac gctgcatccg acagccgttg
    25321 cctgatgtga cgccagcgcg tcttatcagg cctgtgctgg agcagaactg taggtcggat
    25381 aaggcgttca tgcggcatcc gacacgcatt gcccgatgcc gcaaaggcat aaaaagtcga
    25441 tggcgttgaa tattttttca gcgccatttt tattgatgcg cgggaaggaa atccctacgc
    25501 aaacgttttc tttttctgtt agaatgcgcc ccgaacagga tgacagggcg taaaatcgtg
    25561 ggacacatat ggtctggatt gattacgcca taatcgcggt gattgctttt tcctctctgg
    25621 ttagcctgat ccgcggcttt gttcgtgaag cgttatcgct ggtgacatgg ggttgtgctt
    25681 tctttgttgc cagtcattac tacacttacc tgtcagtctg gtttacgggc tttgaagacg
    25741 aactggttcg aaatgggatt gccatcgcgg tactgtttat cgctaccctg atcgttggtg
    25801 ctatcgtgaa cttcgtgata ggccagttgg tggagaaaac ggggttgtca ggcaccgatc
    25861 gggtgctggg cgtctgtttc ggtgcgttgc gcggtgtgtt gattgttgcc gccattctct
    25921 tctttctcga ctcctttacc ggggtgtcga aaagcgaaga ctggagcaaa tcacagctga
    25981 tcccgcagtt cagttttatc atcagatggt tttttgatta tctgcaaagc tcgtcaagtt
    26041 tcttgcccag agcgtaagtg ctctgagatg tggcttaacg aggaaaaaga cgtatgtgcg
    26101 gtattgtcgg tatcgccggt gttatgccgg ttaaccagtc gatttatgat gccttaacgg
    26161 tgcttcagca tcgcggtcag gatgccgccg gcatcatcac catagatgcc aataactgct
    26221 tccgtttgcg gaaagcgaac gggctggtga gcgatgtatt tgaagctcgc catatgcagc
    26281 gtttgcaggg caatatgggc attggtcatg tgcgttaccc cacggctggc agctccagcg
    26341 cctctgaagc gcagccgttt tacgttaact ccccgtatgg cattacgctt gcccacaacg
    26401 gcaatctgac caacgctcac gagttgcgta aaaaactgtt tgaagaaaaa cgccgccaca
    26461 tcaacaccac ttccgactcg gaaattctgc ttaatatctt cgccagcgag ctggacaact
    26521 tccgccacta cccgctggaa gccgacaaca ttttcgccgc catcgctgct acaaaccgct
    26581 taatccgcgg tgcgtatgcc tgtgtggcga tgatcatcgg ccacggtatg gttgctttcc
    26641 gcgatcctaa cgggattcgt ccgctggtac tgggaaaacg tgatattgac gagaaccgca
    26701 cagaatatat ggtcgcttcc gaaagcgttg cgctcgatac gctgggcttt gatttcctgc
    26761 gtgacgtcgc gccgggcgaa gcgatttaca tcactgaaga agggcagtta tttacccgtc
    26821 agtgtgctga caatccggtc agcaatccgt gcctgtttga gtatgtatac tttgcccgcc
    26881 cggactcgtt catcgacaaa atttccgttt acagcgcgcg cgtgaatatg ggcaccaaac
    26941 tgggcgaaaa aattgcccgc gaatgggaag atctggatat cgacgtggtg atccctattc
    27001 cggaaacctc gtgtgatatc gcgctggaaa ttgcgcgtat tctgggcaaa ccgtaccgcc
    27061 agggcttcgt taaaaaccgt tatgttggcc gcaccttcat catgccgggt cagcagctgc
    27121 gtcgtaagtc cgtgcgccgt aaactgaacg ccaaccgcgc cgagttccgc gataaaaacg
    27181 tcctgctggt cgacgactct atcgtccgtg gcaccacttc tgagcagatt atcgagatgg
    27241 cacgcgaagc cggagcgaag aaagtgtacc tcgcttctgc ggcaccggaa attcgcttcc
    27301 cgaacgttta cggtatcgat atgccgagcg ccacggaact gatcgctcac ggtcgcgaag
    27361 tagatgaaat tcgccagatc atcggtgctg acgggttgat tttccaggat ctgaacgatc
    27421 tgatcgaagc cgttcgcgct gaaaacccgg atatccagca gtttgaatgc tcggtgttca
    27481 acggcgtcta cgtcaccaaa gacgttgatc agggctacct cgatttcctc gatactttac
    27541 gtaatgacga cgccaaagca gtgcaacgtc agaacgaagt ggaaaatctc gaaatgcata
    27601 acgaaggatg atgccttcac tcagggtgcc ggtctggcac cctgacttgc aactcccgcc
    27661 gaaatcctgc aaagtctgcc tgcaagtctg acagggcaac tatttatgaa acgactcatt
    27721 gtaggcatca gcggtgccag cggcgcgatt tatggcgtgc gtttattaca ggttctgcgc
    27781 gatgtcacag atatcgaaac gcatctggta atgagccagg cggcgcgcca gaccttatcc
    27841 ctcgaaacgg atttttccct gcgcgaagtg caggcgttag ctgatgtgac acacgatgcg
    27901 cgcgatattg ctgccagcat ttcttccggt tctttccaga cgttggggat ggtgatttta
    27961 ccctgttcaa tcaaaaccct gtccggcatt gtccatagct atactgatgg cttactgacc
    28021 cgtgcggcag atgtggtgct gaaagaacgt cgcccgttgg tgctctgcgt gcgtgaaacg
    28081 ccactgcact taggccatct gcgtttaatg actcaggcgg cagaaatcgg tgcggtgatt
    28141 atgcctcccg ttccggcgtt ttatcatcgc ccacaatccc ttgatgatgt gataaatcag
    28201 acggttaatc gtgttcttga ccagtttgcg gtaacccttc ctgaagatct ctttgcccgc
    28261 tggcagggcg cataactccg ctgttgccct gtttcagggc aattttgcaa ccgcgatcaa
    28321 atcctcgaca ttttgtttct gccattcaat cgaaacgctg cgattcaatc gctatacctg
    28381 ctatcttcaa ctttaggaca ataatgcaac gtcttattaa catatttaac gttgaatgtt
    28441 actgctgtcg tcaagatggc ataagacctg catgaaagag cctgcaaaca cacaacacaa
    28501 tacacaacat aaaaaagcca ttttcacttg agggttatgt atgaagaagt cgattctcgc
    28561 tctgtctttg ttagtcggtc tctccgcagc ggcttccagc tatgcggcgc taccggagac
    28621 ggtacgtatc ggaaccgata ccacctacgc accgttctca tcgaaagatg ccaaaggtga
    28681 ctttgttggc tttgatatcg atctcggtaa cgagatgtgc aaacggatgc aggtgaaatg
    28741 tacctgggtt gccagcgact ttgacgcgct gatcccctca ctgaaagcga aaaaaatcga
    28801 cgccattatt tcgtcgctct ccattaccga taaacgtcag caggagatag ccttctctga
    28861 caagctgtac gccgcagatt ctcgtttgat tgccgccaaa ggttcaccga ttcagccaac
    28921 gctggattca ctgaaaggta aacatgttgg tgtgctgcag ggatcaaccc aggaagcata
    28981 cgctaacgag acctggcgta gcaaaggcgt ggatgtggtg gcctatgcca accaggattt
    29041 agtctattcc gatctggctg caggacgtct ggatgctgcg ttacaagatg aagttgctgc
    29101 cagcgaaggt ttccttaagc aacccgccgg taaagatttc gcctttgcag gcccatcagt
    29161 gaaagacaaa aaatacttcg gtgacggcac cggtgtaggg ctgcgtaaag atgatgctga
    29221 actgacggcg gccttcaata aggcgcttaa cgagctgcgt caggacggca cctacgacaa
    29281 gatggcgaaa aagtatttcg actttaatgt ctacggtgac tgatacgtcg cagggaagct
    29341 gtacctgatg gaatgatcat catggtgcac gccaggtttg ttgcactatc gtggtgcatt
    29401 gaaatgcata cttaagcatt tttaatgaaa aataatacgt ctggcggggc gggatatttt
    29461 gccttgatgg tcaattttat ggcacgataa gtgtaacaaa cctgtaaata ttcccgataa
    29521 aaagactgtc agttgaggac attatgaaaa aactggtgct atcgctctct ctggttctgg
    29581 ccttctccag cgcaactgcg gcatttgctg cgattccgca aaacatccgc atcggtaccg
    29641 atccgaccta tgcgccattt gaatcaaaaa attcacaagg tgaactggtt ggcttcgata
    29701 tcgatctggc gaaggagttg tgcaaacgca tcaatacgca atgtacgttt gtcgaaaacc
    29761 cactcgatgc gttaatcccg tccttaaaag cgaagaagat tgacgccatc atgtcatcgc
    29821 tttccattac ggaaaaacgt cagcaagaaa tagccttcac cgacaaactg tacgctgccg
    29881 attctcgtct ggtggtggcg aaaaattccg acattcagcc gacagtcgaa tcgctgaaag
    29941 gcaaacgggt aggcgtattg cagggcacca cccaggagac gttcggtaac gaacactggg
    30001 caccaaaagg cattgaaatc gtttcttatc aagggcagga caatatttat tctgacctga
    30061 ctgccggacg tattgatgcc gcgttccagg atgaggtcgc agccagcgaa ggtttcctca
    30121 aacaacccgt cggtaaagat tacaaattcg gtggcccgtc tgttaaagat gaaaaactgt
    30181 ttggcgtagg gaccggcatg ggcctgcgta aagaagataa cgaactgcgc gaggcactga
    30241 acaaagcctt tgccgaaatg cgcgctgacg gtacttacga gaaattagcg aaaaagtact
    30301 tcgattttga tgtttatggt ggttaatcgc catcggtgaa agaaagcccg ctcccttcgg
    30361 gtaaccggag ggagacaaga ggcggtaatt caccacacac gacaggacag gcagcatgtt
    30421 gtatgggttt tcaggtgtta ttttacaggg tgcgctcgtc acgctggagc tggctatcag
    30481 ctctgtagtg ctcgctgtaa tcatcggttt aattggcgct ggcggtaagc tctcacaaaa
    30541 tcggctttcg ggccttattt tcgaagggta caccacgctg attcgaggcg tgccggattt
    30601 agtattgatg ctgctgattt tctacggttt gcagattgcg ctaaacacgg tgacggaggc
    30661 gatgggcgtc gggcagattg atattgatcc gatggtcgca ggtattatca ctctcggttt
    30721 tatctatggt gcttatttta ccgaaacgtt tcgtggcgcg tttatggcag tgccgaaagg
    30781 acatatagag gcggcgacgg cgttcggttt tactcgtggg caagtgtttc ggcggatcat
    30841 gtttccggcg atgatgcgtt acgccctgcc aggcattggc aacaactggc aggtgatcct
    30901 caaatctacc gcactggttt cgttactcgg cctggaagat gtggtcaaag ccacgcaact
    30961 ggcaggcaaa agtacctggg aaccgttcta tttcgctatc gtctgtggcg tgatttatct
    31021 tgttttcacc accgtttcca atggtgtgct gctgttcctt gagcgccgct actccgtggg
    31081 tgtgaagagg gctgacctgt gatcgaaatc ttacatgaat actggaaacc actgctgtgg
    31141 actgacggtt atcgctttac tggcgtggcg atcaccctgt ggctgctgat tttgtcggta
    31201 gtgataggcg gagttctggc gctgtttctg gcgattggtc gcgtctcaag taataaatac
    31261 atccagtttc caatctggtt atttacctat atttttcgcg gtacgccgct gtatgttcag
    31321 ttgctggtgt tctattccgg catgtacacg ctggagattg ttaagggaac cgagttcctt
    31381 aacgcctttt ttcgcagtgg tctgaactgt accgtgctgg cgctgacgct taacacctgc
    31441 gcttacacta ccgagatttt tgccggggca atccgttcag tgccgcatgg ggaaattgaa
    31501 gccgccagag cctatggctt ctcgaccttt aaaatgtatc gctgcattat tttgccttct
    31561 gcgctgcgta ttgcgttacc ggcgtacagc aacgaagtga tcctgatgct gcactctact
    31621 gcgctggcat ttactgccac ggtgccggat ctgctgaaaa ttgcccgcga tattaacgcc
    31681 gccacatatc aaccttttac cgctttcggc attgccgcgg tgctctattt aatcatctct
    31741 tatgtcctga tcagcctctt tcgtagagct gaaaaacgct ggttgcagca tgtgaaacct
    31801 tcttcaacgc actgagaata cgatgtccga gaataaatta aacgttatcg atttgcacaa
    31861 acgctacggc gaacacgaag tgctgaaagg ggtctcattg caagcgaatg ccggagatgt
    31921 cataagcatc atcggatcgt cgggatcggg taaaagtacc tttctgcgct gcattaactt
    31981 cctcgaaaaa ccgagcgaag gatcgatcgt ggtcaatggc cagacgatca atctggtgcg
    32041 cgataaagac ggtcaactca aagtcgccga taaaaatcag ctgcgcttat tgcgcacacg
    32101 cctgacgatg gtattccagc acttcaatct ctggagccat atgacggtgc tggaaaacgt
    32161 catggaagcg ccgattcagg tgttgggcct gagcaagcag gaagcgcgcg agcgggcggt
    32221 gaaatatctg gcaaaagtcg ggatagacga acgtgcgcag gggaaatacc cggtacacct
    32281 gtccggtggt cagcaacagc gtgtttctat cgcgcgggcg ctagcgatgg aaccggaagt
    32341 tttactgttt gatgaaccta cttcagcgct cgatcctgaa ctggtaggcg aagtgttgcg
    32401 tattatgcag caactggcag aagaggggaa aaccatggtg gtagtgactc acgaaatggg
    32461 ctttgctcgt catgtttcta ctcacgtcat tttcctccat caggggaaaa tagaagaaga
    32521 aggggcgccg gagcagttat tcggtaaccc gcaaagccct cgtctgcaac agttccttaa
    32581 aggttcgctg aaataatttc ctctccggtg gttaacgctg ccggagtatt tcatggggaa
    32641 tttgcgagtc cgcttccgct gaaatgtaaa agttgcagtc cgctaaaaat agttctgaat
    32701 tcgctgtcca aagataaaaa ctgcctgctg cattgggtgt aaatcgtggt tattgtcgcg
    32761 tcatcttaaa tttgaggggt gacggaacgc catgacaata tcgacaactt ccacgccgca
    32821 tgatgcggta tttaaatctt ttttacgcca tccagacacc gcgcgggatt ttattgatat
    32881 tcatcttccc gcgccgctgc gcaaactgtg tgatttaacg acgcttaaac tggaaccaaa
    32941 cagttttatt gatgaagacc tgcggcaata ttattccgac ctcttgtggt ctgtgaaaac
    33001 gcaggaggga gcgggttata tttatgtggt gatagagcac caaagtaagc cagaggaatt
    33061 aatggctttt cgcatgatgc gctattccat tgcggcgatg caaaaccatc ttgatgcggg
    33121 ttataaagag cttccattag tgatcccgat gctgttttat catggttgca gaagccctta
    33181 tccatattca ctctgctggc ttgatgaatt tgccgagcct gcgattgccc gcaaaattta
    33241 ttcatcggct tttccgctgg tggatattac cgtggtgccg gatgacgaga ttatgcaaca
    33301 tcgcaaaatg gcgctgttgg agttaattca gaaacatatt cgtcagcgcg atctgttggg
    33361 attagtcgac caaattgttt cgctgctagt tacagggaac actaatgaca gacagctaaa
    33421 agccctgttt aattacgtat tacaaacggg ggatgcccgg cgtttccgtg catttattgg
    33481 tgagatagcg gaacgcgcac cacaagaaaa ggagaaactg atgaccattg ctgacagatt
    33541 acgtgaagaa ggcgcaatgc agggcaaaca cgaagaagcc ctgcgcattg ctcaggagat
    33601 gatggaaaaa ggatttgacc acgaagtcat cctgacgctg acccgacttt caccagacga
    33661 tcttatcgcg caaagccact aaccctgtaa caccgggagt taactggcgg atgtttgcag
    33721 taaaccacat cagcgaacga catccgccag cgcctcttct aaatcgtacc agcgaaacgc
    33781 aaaacccgct tcttccagct tttttggcag cgcgcgttga ccgcctaata ccagaactga
    33841 agattcgccc attaacaacc gaatggcggt cgcagggacg cgcaaaatgg ccgggcgatg
    33901 cagcgcatga cccagtgtat gggcaaattg ttcattgcgc accgggtagg gcgaaaccat
    33961 attaaatggc ccgcgcagct ctttatccag cagccagaga ataccgttga ccatatcatc
    34021 aatatgaatc caggccagat actgccgacc agagccaatc ggcccgccca ggccaaaacg
    34081 aaatggcggc agcattttac cgaggatacc accgtctggt gccagcacta cgccggtacg
    34141 cagcaggcac actcgcgttt tgtcactttg cgcccgacag gcaatctctt cccagcgggc
    34201 gcagagttta tgggtaaatt cgttatgcgg cggttcctct tcggtaacca ccacttcacc
    34261 taagtcgcca taatagcccg ttgccgagcc ggaaatgagt accgacggcg gcgtgtcgct
    34321 ggcattaatc aaatcgacca gtttttgcgt gatattccag cggctttggc agagacgctc
    34381 tttttgctcg tgagtccagc gtttatcagc aatcggttct ccggccaggt tgattaccgc
    34441 atcaacgccg ttgaggttgc tttgttcggc aagcccttgc catagtgtca cccgagggcc
    34501 gagaacggaa ctggctttct gcgggttacg cgtcactacc gtgatttgat ggcccaggtc
    34561 cagcaaacgt ggaatcaaat ggcgaccaat taatcccgtc cctccggtga tcactatgtt
    34621 catgcagcct cccgatatgg cggttaacgc tgccagctta aggtcatgga taccgaatcg
    34681 gcgtagcgca gcgcgtgcag tttatcgatc tccacttcag catacgtcac ccagtgatgt
    34741 tcacgtgcga tatcgagcac atcctgagtt aatttttcca gcaaagagaa acggttattc
    34801 tctacatgct gaataatgtt tttcgttacg gtgcgataat tcagcgcatc gttgatatct
    34861 tcgctagtgc gcgctttatc ggcggggtag tggatagtca cattgataac aatatcctga
    34921 cggttgttaa tttcttcttc cttaattccg ataaacgtac gcaaacgaag gttctttata
    34981 cgaataatag cggcaggttg tgccattgca ggttctccgt tgttttactt agaatgaaat
    35041 aatacacgag aacctgtttc tgttaactat ccgaacgctc atcaccgtgt tgtgctttta
    35101 gcagtgcctg cccggtggca gggcgcgaac ggatccgctc atgccagttc ttgactgccg
    35161 gatacattgc gaggtcaatt cgctggcgag tccaggcatt aacccacggc cagcaggcaa
    35221 tatccgcaat gctgtagttc tcgccaccca gccagggcga gttttccagc cgcttgttca
    35281 gtacatggta cagacgctga gtttcaacct gataacgttc aatagcgtaa ggaatggttt
    35341 ggggggctgc gtgattaaaa tgatgatttt gcccaagcat cggccccagt ccgcctacct
    35401 gccagaataa ccactgtaat gtgacggcgc gttcacgcgt ttcatgactc aaaaagagtc
    35461 ccgttttctc agccagatac aacaaaatgg caccagactc aaagaggctt agcggttcgc
    35521 cgccatcggc tggagaatga tcaacaattg ccggaatttt gttgttaggc gaaatgcgca
    35581 aaaattccgg gcgaaactga cctcctttcc ccaggtctac ctttattaag cgataatcca
    35641 gccctgcttc ttcgagaaac agcgtaattt tgtgaccatt gggtgtcggg gcgaaataga
    35701 gatcgatcat tgcagctccg gttgtctaat catctgaaca ccacaagtat agaaagcgcg
    35761 gcgacttgcg tgagtattcg tcacgtgaca gtgacaaaaa ggctctatag tttgatagca
    35821 agcccccttt attgccgagg acataatgag taaacccgct atcacgcttt ggtcagatgc
    35881 ccattttttc tccccttatg tgttatccgc ctgggtggcg ttgcaggaga aaggcctgtc
    35941 gtttcatatc aagaccatcg acctcgacag cggtgaacat ttgcagccga cgtggcaagg
    36001 ttacggtcag acacgccgtg tgccgttatt acaaatcgat gattttgagt tgagtgaatc
    36061 ttctgccatt gcggagtatc tggaagatcg atttgcgcca ccgacctggg aacgtattta
    36121 tccgcttgat ttagaaaatc gtgcgcgagc acgacagatt caggcctggc tgcgcagcga
    36181 tctgatgccc atccgcgaag aacgcccgac ggatgttgtc tttgcggggg cgaaaaaagc
    36241 gccactaacg gccgagggaa aagccagtgc agagaaactg ttcgcgatgg cagaacattt
    36301 gttagcactg ggtcagccga atttatttgg tgaatggtgc attgctgata ctgatctggc
    36361 gctaatgatt aaccgcctgg tactacatgg cgatgaggtg ccggaacgcc tggtggatta
    36421 tgcgacattc cagtggcagc gagcgtctgt ccagcgtttt attgcacttt cggcgaagca
    36481 atctggctga tagcaacgac agaatccagt atcatagctt acgttttttc gacgaggagt
    36541 gagtgatgaa actgatgttt gcatcggaca ttcatgggtc gttaccggcg acggaacgtg
    36601 ttctggagtt gtttgtccaa agcggtgccc agtggctggt gatccttggc gacgtgttga
    36661 atcatggccc gcgtaatgct ttaccggagg gttacgcgcc agccaaagtc gctgaacggc
    36721 ttaatgaagt ggcacataag gttatcgctg tgcgcggcaa ctgcgacagc gaagtggatc
    36781 aaatgctgct gcatttcccg ataaccgcgc cgtggcaaca ggtattactg gaaaaacaac
    36841 gtctgttttt gacgcatggt catctttttg gcccggaaaa tctacctgct ttaaaccaga
    36901 acgatgtgct ggtgtacggt catacccatc tgccagtggc agaacagcgg ggggagattt
    36961 tccacttcaa ccccggctcg gtgagtattc cgaaaggcgg taatccggcg agttatggca
    37021 tgctggataa tgacgtactt agtgtaatcg cactcaatga tcaaagtatc attgcgcagg
    37081 tcgcgattaa tccgtaattt acccacaact caaaatgcgc cgtaagagcg ccagaaaaag
    37141 aaggtttcct gataatggaa cagcgtcgtt tggcaagtac tgaatgggtg gatattgtca
    37201 atgaagagaa cgaagtcatt gcacaagcca gccgggaaca aatgcgggca cagtgtctgc
    37261 gtcatcgtgc aacttatatc gtcgtgcatg atggcatggg caaaattctg gtccagcgtc
    37321 gtaccgagac aaaagacttt ttacccggca tgttagatgc gaccgcaggc ggtgtagtcc
    37381 aggccgatga gcagctgctg gaatccgcgc gtcgcgaagc ggaagaagag ttgggcattg
    37441 ccggtgtccc ctttgccgag cacgggcagt tctatttcga agataaaaat tgccgtgtct
    37501 ggggcgcgtt gttcagctgc gtctctcacg gtcccttcgc tctacaggaa gatgaagtca
    37561 gtgaagtttg ctggctgacg ccggaagaaa tcaccgcacg ctgcgatgag ttcactccag
    37621 actcgctgaa agcgctggcg ttgtggatga agcgcaatgc caaaaatgaa gccgtagaga
    37681 ctgaaacggc agaatgaaaa aagagccgca atcgcggctc ttaacatttt tagtggtagc
    37741 ccatcaactg agcgccgatc accacgacgc tggacataat aaacagcagt ccaagcaggg
    37801 tcgcacccac cttcagccag ttacggaagt ccacccggca aacaccgagc gtcgccatta
    37861 acgaagctga ggttgggtaa atgatgtggc tgaagccatc accaaactgg aacgccagca
    37921 cggtaacctg acggttaaca ccgaccagat cgccaagcgg tgccagtaac ggcatggtta
    37981 acgccgcctg accagaaccg gacgtcacga agaaattaaa taccgcctgg aagagcaaca
    38041 taaaccaggc cgcgactgcg ttatccagac cgctaatggc attggcaatg ctgttgagga
    38101 tggtatttaa cacgctggca tcacccgctt caccattacc gaccagcagc aaaatccctt
    38161 tggcgaaacc caccagcagg gcaggggcga tcatcattcg cgccccttcg gtaaaggatg
    38221 aagccatggt attaaccgtc atgccgttaa ggcggaaaac aacgccgatg atgccaatca
    38281 ccagacccat ggtgaagaac tggctggcaa tttcaggaat aaaccaggca ttaacgatca
    38341 cgccccaaat aacccagacc attacggcgg tcaggacaat caataccagc cagtcaccaa
    38401 aggtaaacgg acgttgttca acatcagcct gcttttcacg aaagaagcgg tcggactcat
    38461 gcacgcgtga cagaagagga ttctttttca ctcgtgaggc gtacaccatg gtaaagatca
    38521 ggccaatcag agtggcgata acccacacca cgatgcgcaa cccggagcca gaaagcaccg
    38581 gaacgccggc aataccctga gcaacaacca cacaaaacgg gttcatccac gaactggcaa
    38641 aaccgatttg cgtggcaata taggtcacaa ggacggtggt aatactgtca tagcccagcc
    38701 ggaccattag cggtgcgatg ataatggcaa aggcgacggc ctcttctccc ataccaaata
    38761 ttgcgccgcc aagtgaaaac agaataaaca gcgcaggaat aaagagaatt tcatttccgc
    38821 gggtatggcg aataagcgcc aggataccgt tatcaatggt tcctgtacgc atcacaatgc
    38881 caaacgcgcc gccaatcacc agcataaaca tgatgatgcc aacggctgtc ccgtatttcg
    38941 atcctgaggt taacccttca aacgggaagt tcatcagacc cgggcgttca tcgcccgtcg
    39001 tgaacagctg tacgcggtga tactcaggtt cgcctgcttc gttagtcaga atgcgaaatg
    39061 agtgtggatc tacgactttg cgtgtttttg tttgaccatc aacctgatac tgcacttcct
    39121 gactgtcaaa catgcccacc ggaactaccc aggtggcaag gctggttaaa atggcaacaa
    39181 aaaagataat caccaacgta tcgggcattg cccatcttct tgttggttta gattcagtga
    39241 ttgcggacat agcgcaaata ttcccttgca caaaacaaag tggtaagtat gcaaagtgag
    39301 atggcgcaat tcattgatgc agcgcagtta agcaagataa tcagaaagga ttaatgcaaa
    39361 ttaataga
//
LOCUS       DAZFRE010000036        39195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFRE010000036 DAZFRE010000000
VERSION     DAZFRE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.036"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            258..815
                     /gene="hxlB"
                     /locus_tag="ACHKUI_002209"
     CDS             258..815
                     /gene="hxlB"
                     /locus_tag="ACHKUI_002209"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFO6598396.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            857..2365
                     /gene="ptsG"
                     /locus_tag="ACHKUI_002210"
     CDS             857..2365
                     /gene="ptsG"
                     /locus_tag="ACHKUI_002210"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO6598397.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMAKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            2416..4413
                     /gene="tkt"
                     /locus_tag="ACHKUI_002211"
     CDS             2416..4413
                     /gene="tkt"
                     /locus_tag="ACHKUI_002211"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6598398.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGRFAEDTAARFRAYGWHVIEGVDGH
                     DPEEVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(4443..5288)
                     /locus_tag="ACHKUI_002212"
     CDS             complement(4443..5288)
                     /locus_tag="ACHKUI_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6598399.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(5489..6394)
                     /locus_tag="ACHKUI_002213"
     CDS             complement(5489..6394)
                     /locus_tag="ACHKUI_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6598400.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(6472..7695)
                     /locus_tag="ACHKUI_002214"
     CDS             complement(6472..7695)
                     /locus_tag="ACHKUI_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6598401.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFTVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(7721..8110)
                     /locus_tag="ACHKUI_002215"
     CDS             complement(7721..8110)
                     /locus_tag="ACHKUI_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO6598402.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(8127..9083)
                     /gene="arcC"
                     /locus_tag="ACHKUI_002216"
     CDS             complement(8127..9083)
                     /gene="arcC"
                     /locus_tag="ACHKUI_002216"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6598403.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKMAINFGTPQQQWLDAISVAEAQTLSREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(9076..10500)
                     /locus_tag="ACHKUI_002217"
     CDS             complement(9076..10500)
                     /locus_tag="ACHKUI_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6598404.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDHGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVVKENHHG"
     gene            complement(10497..12056)
                     /gene="fdrA"
                     /locus_tag="ACHKUI_002218"
     CDS             complement(10497..12056)
                     /gene="fdrA"
                     /locus_tag="ACHKUI_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6598405.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNV"
     gene            complement(12151..13011)
                     /locus_tag="ACHKUI_002219"
     CDS             complement(12151..13011)
                     /locus_tag="ACHKUI_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6598406.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGRMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQAPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(13017..13685)
                     /locus_tag="ACHKUI_002220"
     CDS             complement(13017..13685)
                     /locus_tag="ACHKUI_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFO6598407.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGTMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(13942..14751)
                     /locus_tag="ACHKUI_002221"
     CDS             complement(13942..14751)
                     /locus_tag="ACHKUI_002221"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6598408.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            15013..15777
                     /gene="udp"
                     /locus_tag="ACHKUI_002222"
     CDS             15013..15777
                     /gene="udp"
                     /locus_tag="ACHKUI_002222"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6598409.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(15812..17302)
                     /locus_tag="ACHKUI_002223"
     CDS             complement(15812..17302)
                     /locus_tag="ACHKUI_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO6598410.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(17315..17821)
                     /locus_tag="ACHKUI_002224"
     CDS             complement(17315..17821)
                     /locus_tag="ACHKUI_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO6598411.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(17838..18812)
                     /locus_tag="ACHKUI_002225"
     CDS             complement(17838..18812)
                     /locus_tag="ACHKUI_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO6598412.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLSLKK"
     gene            complement(18837..19466)
                     /locus_tag="ACHKUI_002226"
     CDS             complement(18837..19466)
                     /locus_tag="ACHKUI_002226"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6598413.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(19456..20460)
                     /locus_tag="ACHKUI_002227"
     CDS             complement(19456..20460)
                     /locus_tag="ACHKUI_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6598414.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCSLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(20484..21251)
                     /locus_tag="ACHKUI_002228"
     CDS             complement(20484..21251)
                     /locus_tag="ACHKUI_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6598415.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGTFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            21468..22895
                     /gene="rmuC"
                     /locus_tag="ACHKUI_002229"
     CDS             21468..22895
                     /gene="rmuC"
                     /locus_tag="ACHKUI_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6598416.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            22990..23745
                     /gene="ubiE"
                     /locus_tag="ACHKUI_002230"
     CDS             22990..23745
                     /gene="ubiE"
                     /locus_tag="ACHKUI_002230"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6598417.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            23759..24364
                     /gene="ubiJ"
                     /locus_tag="ACHKUI_002231"
     CDS             23759..24364
                     /gene="ubiJ"
                     /locus_tag="ACHKUI_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6598418.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQCYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            24361..26001
                     /gene="ubiB"
                     /locus_tag="ACHKUI_002232"
     CDS             24361..26001
                     /gene="ubiB"
                     /locus_tag="ACHKUI_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6598419.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            26080..26349
                     /gene="tatA"
                     /locus_tag="ACHKUI_002233"
     CDS             26080..26349
                     /gene="tatA"
                     /locus_tag="ACHKUI_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6598420.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            26353..26868
                     /gene="tatB"
                     /locus_tag="ACHKUI_002234"
     CDS             26353..26868
                     /gene="tatB"
                     /locus_tag="ACHKUI_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6598421.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            26871..27647
                     /gene="tatC"
                     /locus_tag="ACHKUI_002235"
     CDS             26871..27647
                     /gene="tatC"
                     /locus_tag="ACHKUI_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6598422.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            27689..28471
                     /gene="tatD"
                     /locus_tag="ACHKUI_002236"
     CDS             27689..28471
                     /gene="tatD"
                     /locus_tag="ACHKUI_002236"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6598423.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(28468..28956)
                     /gene="rfaH"
                     /locus_tag="ACHKUI_002237"
     CDS             complement(28468..28956)
                     /gene="rfaH"
                     /locus_tag="ACHKUI_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6598424.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            29123..30616
                     /gene="ubiD"
                     /locus_tag="ACHKUI_002238"
     CDS             29123..30616
                     /gene="ubiD"
                     /locus_tag="ACHKUI_002238"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6598425.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRVTLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWSEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            30662..31363
                     /gene="fre"
                     /locus_tag="ACHKUI_002239"
     CDS             30662..31363
                     /gene="fre"
                     /locus_tag="ACHKUI_002239"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6598426.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(31635..32798)
                     /gene="fadA"
                     /locus_tag="ACHKUI_002240"
     CDS             complement(31635..32798)
                     /gene="fadA"
                     /locus_tag="ACHKUI_002240"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6598427.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTSGHDADGVLKQFNYDEVIRPETTVEA
                     LTTLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMDHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(32808..34997)
                     /gene="fadB"
                     /locus_tag="ACHKUI_002241"
     CDS             complement(32808..34997)
                     /gene="fadB"
                     /locus_tag="ACHKUI_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6598428.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAAMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEQYYPPVEPARPVGDLKTA"
     gene            35187..36518
                     /gene="pepQ"
                     /locus_tag="ACHKUI_002242"
     CDS             35187..36518
                     /gene="pepQ"
                     /locus_tag="ACHKUI_002242"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6598429.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIANMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            36518..37132
                     /locus_tag="ACHKUI_002243"
     CDS             36518..37132
                     /locus_tag="ACHKUI_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6598430.1"
                     /translation="MESWLIPAAPVMVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            37171..38622
                     /gene="trkH"
                     /locus_tag="ACHKUI_002244"
     CDS             37171..38622
                     /gene="trkH"
                     /locus_tag="ACHKUI_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6598431.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            38634..39179
                     /gene="hemG"
                     /locus_tag="ACHKUI_002245"
     CDS             38634..39179
                     /gene="hemG"
                     /locus_tag="ACHKUI_002245"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6598432.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         9315 a         9843 c        10444 g         9593 t
ORIGIN      
        1 ttgttcggtg tatagccaga gacgagttca gtttgtgatt tttacagggc agttacagcc
       61 gcaaaatttt atggctgatt tcaccttccc ctcaagctta tctttcttaa aaagagaaat
      121 aattcttaaa ttcaaatagt tatatacaat ctactctctt tcttgtgact agcttcacat
      181 ttcatactga tgcattggaa tgaaataaaa tttcatgaga ttttgtgttt gagaaaaatt
      241 atttcatgag gtgtgaaatg gacgcaaagc tgtacatcac tgagatcatc aatgaattaa
      301 ccttcgttaa ggactctatc cgtgaggatg actgcaccaa attgatcgac gctattcagc
      361 aaagccagcg cgttttctgc tatggcctgg gacgtgccgg cttttccatg aaagccttca
      421 ccatgcgcct gatgcatatg ggaaaagagg tttacttcct gacggaaacc attacgccga
      481 actttgggcc tggcgatctg tttatcgttt cttccgcttc cggtgagaca gcacaactgg
      541 tggcactggc gaaaaaagca cgtcagcttg gcggcgctgt ggcggtgtta accacgaatc
      601 gacacgccac tatcaccgag tttgtcgatg tcatcgtgca aattaatgcc ccttccaaaa
      661 atcaaaaaga ttctgttttc cgctcggcac agccgatggc gtcgttgtat gagcaagctt
      721 tgctggtgat tgccgatgca ttagtgatga agatggctgc tgaaagtggt gcgccggaaa
      781 gtgaactgtt taagcgccac gccaatctgg aatagtcgtc gccgttattt gctcgatacc
      841 ctacaggaga ataaaaatga gcaaaaaaat aacatccttg tttaacttca agaagttgca
      901 gaaattttct tctgctctga tgttaccaat cgccattctt ccggcggcgg gtttgatttt
      961 agccatcggg atttcattta acattgagct gctggcgaat tgcggcggcc tgattttcgc
     1021 caaccttcct ttgctgtttt gtgtcggtat tgtgattggc ctgacaggtg atggttcttc
     1081 ttcactttca gcgattatca gctacatgat catgaatgcc acgatgggta cgcaacttgg
     1141 cattaccgcg gagtccgttg cagccagtaa cggtgactat gcgatgatcc tcggggtacc
     1201 gacgttacaa acgggagtgt ttggcggttt gattgtcgcc atctttgttt atctgctcta
     1261 tacccgcttc cacaatattg aattgcctca gtttcttggt ttcttcggtg gaaaacgctt
     1321 tgtaccgatt attacggcgg tggcggcaat tattgtcggc tatttattgc cgtggctgtg
     1381 gataccggta caaaaaggtc tggcgtcgct ttcaacgctg gtgaccagtg ggcataacgc
     1441 gaacttcgcg gcctttattt acgccgttgg cgaacgttcg ctgattcctt ttggcctgca
     1501 tcatatctgg aacgtgccgt tttattacaa ctttggcgac tacatggcta aatccgggca
     1561 actggtgacg ggcgatattc cggtgttctt tgcccaattg cgcgacggtg ttccgttgac
     1621 ggcggggctg tttatgacag gtcgtttccc gatcatgatg tttgcgttac ctgccgccgc
     1681 gttggcgatg tatcaggaag caaaacctga acgtaaggcg ctgattaaag gccttttact
     1741 ctccggtgcg ctgaccacat ttgttaccgg gattacggag ccgctggaat atgcattttt
     1801 gtttgcagct ccggttctct acatcattca catcttctta tatgccactt catttgtgct
     1861 gatgaacatc ctgaatgtgc acattggtca tccttttgct ggcggtctta ttgatttcgt
     1921 actgaatggc gttatgccaa accgcacgcc gtggtatctg gtgttcctgg caggcgcggg
     1981 ctatgcagcg atttactaca ccgtatttcg ggtactgatc cgcaagttga atctgaaaac
     2041 accgggacgt gaggatgaag agattgccgt tgcaaccaca ttaacgcgcg aagaacgtcc
     2101 ataccaaatc atcgaagcag taggtggttt cgacaatatc gaagatgtgg atgcctgcgc
     2161 aacacgcctg cggctggcgc tggtagacga taagaaagtg aatgaaaaac ggctgaagga
     2221 gttgggggct gcgggtttag tgaaactggg tgatggcggt gtacaggtga tatttggcgg
     2281 caaatcgcaa atcctgcgcg acgaaatcaa aaccgtaatg agcagaccgc gcccgacaga
     2341 aatgacagtg gcctgtgcta actaattcga gattagcggg ctggtttcag cccgccagac
     2401 agagagagct gaagtatgaa ttcacaatta ctggctaatg ccatccgaat gttaagtgtt
     2461 gatgcgatcc agaaagcgaa ctccggtcat ccgggcgcgc cgatggggat ggcggatatt
     2521 gccgaagtgg tctggcgtcg tcacctgcgt cataacccaa aaaatccgca gtggtttaac
     2581 cgcgaccgct atgttcagtc gaacggtcat ggctccatgt tgatttacgc tttgctgcat
     2641 cttacaggct atgacctgtc gatggatgat attcgcgact ttcgtcagct ccactcccgc
     2701 acgccgggtc atccagaata tggttatacg ccgggtgttg aaaccaccac cggcccgttg
     2761 gggcagggcg tagctaatgc ggtggggatg gcgattgcgg agaaggcgct ggcggcagaa
     2821 tttaacaaac cgggctttaa tattgttgat catcacacat ggctattttt gggtgatggc
     2881 tgcctgatgg aagggatctc ccacgaggcc tgtggtcttg caggaacgct gaagctcggc
     2941 aatctgattg ctatctggga cgataacggt atttcgatcg atggtcacgt agaaggcagg
     3001 tttgctgaag atacggctgc ccgttttcgt gcctacggct ggcatgtcat tgaaggcgtt
     3061 gatggtcacg atccagaaga ggtagatgct gcggtgcgtg aagcgaaaag cgtcacggat
     3121 aagcccagtc tgctgtgctg caaaacgatt atcggttttg gttcgccaaa taaagccaac
     3181 agccacgatt gccacggctc ggcgctgggt gcagatgaag tggcgctggt gcgtgaacgt
     3241 ctgcaatggc cgtatgcccc tttcgaaatt cctggtgaaa tttatgccga atgggatgcg
     3301 accgagaaag gcgcgcaggt tcaacaggaa tgggatgcac tgtttgccga ctacgcgaag
     3361 cagtggccgg aactggcggc ggagtttact cgtcgcatga agggtgattt gcctgctggc
     3421 tgggtcgaga atatgcagaa atatgtgcac gatctgcaat cccatccggc ggcgctggca
     3481 acccgtcagg ttagccagaa atgcctcaat cacttcgcag atatgctgcc ggaactgatg
     3541 ggcggctcgg cggacttgtc gccctctaac ctgactcgcc atcagaaatc ggttgatttc
     3601 accggagaaa atccggcggg gaattacatc tcctatggcg tgcgtgaatt tggtatgtcg
     3661 gccatcatga acggtcttgc gctgcatggt gggtttatcc cttacggcgg tactttcctg
     3721 atgtttatgg aatatgcgcg taatgcactg cgcatggcgg cgctgatgaa gatccgctcg
     3781 gtgtttgttt atacccacga taccatcggc ctgggcgaag acggcccgac acatcagccc
     3841 gttgagcaac tggcgtcgct gcgtctgacg ccgaatatgg aaacctggcg cggttgcgat
     3901 caggtggaag tggcggtggc ctggcaacag gcgattgagc gcaaagatgg cccaacgtcg
     3961 ttggtattaa cgcgccaacc tctggcgcaa cagccgcgca ccgctgcaca gttggcagag
     4021 attgcacgtg gtggttatgt actgagtgat tgtgacggtc agcctgagat gatcctcatt
     4081 tctgcgggtt cagagattga gttagtggtc agcgccgcaa aagcgttaac ggaagaaggg
     4141 cgtaaagttc gcgtggtttc tatgccgtgt accgagcgtt tcgataatca ggatgcggcg
     4201 tacaaagaat cggtattgcc aaaagctgta cgcaaacgtc tggcggtaga agccagcatc
     4261 gccggattct gggaacgcta cgtcggcctg gacggtaaag tgatcggcat gacgagcttt
     4321 ggtgagtctg ctccggcgaa tgtgttgttc aaacactttg gttttacgcc ggaaaatgtc
     4381 ctggcgcagg cgcgtgagct actgaacagc taaacctgca caaggggtga aaggatgacc
     4441 ttttacccct tgaaacggga gccaagaaac tcttcacggg tgcggtattt ctcgtaatgc
     4501 gccttgtttt tatacataaa caacagcacc agcatatcaa taacgcccat tgccggaacg
     4561 cgagtggagt cgaaggcttc aaagaagatt ttatcgctga ccgcggtatt gagttgatgg
     4621 gtggagagtt ttgataacgg agatttaata tagttagtga tacaaacgga tttggcaccg
     4681 cgctctttca ccactttaaa gatatcgacc attaagttgg tgctgccaga gttagaaata
     4741 ccaatcgcca ggtcgcgttt ccccatagaa tatgccgcat taatttgcat ttccgggctg
     4801 acgtgaacaa cggtttttaa tccgagttgg ctgaatttat aagcactata aacggccagt
     4861 gggtttgaat ttccggcagc aaaaaagaaa atagtatcgc aattagcaag tagtttgaga
     4921 atggactcaa gcacgtcggc attaaaaaat tgcgttgatt gtattaaatc ttcggcctgt
     4981 ttttttaata agaaggttgc caaagatgtt atatcgctga agtcggtatt ttcaggatag
     5041 ctattgtcag gattaatgac ttcttttgct aatgcaattt ttaaatggta ataaccctga
     5101 taacctaatt tcttacaggt gcgtacgata gtcgcttcac tggcatggct tttttctgat
     5161 agttcagtga cactatagtt cacgacttca tgtgcgtggc taagaatata gtcagcgacc
     5221 tttttttccg cctgatgcag tgtagggtaa ttgctttgaa tcagactttt tacggttatg
     5281 ttattcataa ttagtaaatt tcattctgac agaaagtgct gattataaca tagttttcag
     5341 gcgtggatag gtgtgaaaaa aatgaaggtg tgcagtgaac gtaatgatta acaggccggt
     5401 taattttcat gaattacgtc aactgcacaa aaataattta aacgggtgct ctaattaaag
     5461 caagacttat atttttatca ttgtattgtc aattaaatac aaatcgtgtc attaattgtg
     5521 ggtttcttaa atattctatg caccactgac aggctttgcc cataccatcg gaacgagcgg
     5581 caagaaataa cttggtggcg tgtttatgtt cgcgcattgg ctttttaagc aatttaccgc
     5641 tgttcaataa cggcaatgcc agatgatgtg gaatataacc aataccgacg ttttgttcta
     5701 ttaaggcgat tgccgtagcg aagtcaggaa caaaaatggg tttttgtcct tccaacaacc
     5761 aggcttgcat cggggggaag ttaacggagg tatcgcggat acaaaccgct ggataatgac
     5821 gcaattcact gttttctaat ggatgccgct tcgctgccag tgggtggagt gggctgacga
     5881 caaaatccca ttccatttgt ccgaccggtt cgctaatgat gccttcactg cttggtactg
     5941 catgaggagc accgtagacc agatcagcac gcttactgta cagcgcatcc cagcagccgt
     6001 tgtatacctc aagatctacc gtcagtgttg ttgatgtgaa ttgccgttca aaatctcgaa
     6061 taaatgccac cagcaaatca ccaggaataa tattgtttac cgcaattgtc agttcacgct
     6121 caataccatc gtgaatcagc actgtgttgc gctttaatgc ttccaggtcg ccaagaatag
     6181 ttttgctatg ttcgatgaag taacttcccg ctggcgataa ttcaatatag cgtcccttac
     6241 gaatgaacag ttcaacgccc aggctctctt ccgctttctt gatggtgtaa ctgatggcgg
     6301 aagggacttt atttaactgc tcggctgcgg tggtaatgct ctggtgtctg gcgaccagat
     6361 gaataacacg aagcgtttcg tctgagatga acataaggct ccctggtcag tggtaaagtg
     6421 gctgaatctc tccagattca gccgtaatca ttgcctgagt atacccagga attagcggtg
     6481 ttgacttgcc cgactgagaa tcgcatacgc gatacatgcc accagaatcg agtctaccga
     6541 agggatcgtc gtcagggtga ataagccttt tacggtcata aagccaaccg ctgcgcctat
     6601 gatccatgct gcgaatgtca gcactttaat ctgtgactcc tgggctaaca ctgactcttg
     6661 atagccatta cggcgataaa ggaaaaagtc agcgatataa attccggcaa cgggtggcgt
     6721 ggcgataccc aggaacagca gaaacggaat aaaccacgcc ataatatcca tactgcctac
     6781 gatggcggaa aggataccca gtgccacggt aatctgccat ttgggaaagc gggtaagcag
     6841 tgtggaaacc accagcgtgc cctggaacat attgcccgcg ttaccggtga cgcaggcgaa
     6901 aatcaacaac agcgccgcag gcagaaccgc accgaatacc gccatcgcac ccagtaaaga
     6961 tccctgacca ctaatggcgc tcggcgttgc acccgcccag tagagcagag ggtaggcaac
     7021 cagaaacgtc acgcctgcgg caatcagcgc atgtttgcgg ttatgcacaa aactaccgaa
     7081 atcaggcagt gtggcaacca gcacaataat ggtgccaaca acggtggaaa cggcgacacc
     7141 tgtattcatc tccacaatcg ccgctggtac tgccacttcg gtatgggttg ccacatagag
     7201 gatgtaacac agcaccagcg cgataaccgg cacggcaacc tgggcaattt ttcccagaac
     7261 ggtaaagcca aatgccgtag aggcgacaaa aatgacacag ccagccgcca ctaacagtgc
     7321 cagtggaact tccagtccat gttgagccag taagtcatgt accgaatgac caaacatatt
     7381 ggcggtaacg gcaatccagc cgaacagact tatcgccatc aaaatgttaa tggcgatggc
     7441 ccctttttcg ccaaacgaat atttcacaat gccatacgtt ggcaggcggg ctttttctcc
     7501 cacgctaatg gtgatggctg aaagtaaggt cagaatgacg ccgcctaaca atacgacgct
     7561 aattaaactg ctgctggaaa gttgattgcc gaggcttgag gatgacaaca gtacaggtgt
     7621 gacggcaata cccagcaaaa tcatggcaat acgcaatcct gatgccgtat tcagattctc
     7681 ttctttttta cgcatagtat gttcccttta taaatgcgcg ttacacagta tgaaaatgcc
     7741 cggtgtattt gcgccagcta cccagttctg taatttcccg ttggccttcg gtcagccagt
     7801 tttctgccag tacgcccagc acttcgctac catcggcaag tttgattttg ccaattgcca
     7861 gcccagcggg ttcgctcatt aacagagcgg caaaactggc cagcggtaat tgccaaattt
     7921 ctacgtcgac gtgtgttcct ccttcattca cgcgaatcat tcccggatgg cgatcattaa
     7981 tactccacaa ccggtaatgg gcgtcagttc ggtcttcacg gacaaaaata ccgcccgctt
     8041 tttgcatatt tgggttgagt tccaggccgc gcatcagcgt tccgttaacg gctaaaagcg
     8101 ttgtagtcat tgtgttctcc agagaattaa agggttatcc atgtaccgct ctgatgcgcc
     8161 agggctgctt ttatggtttg cggtgaagta atcaggccgc tggcctgttt gccttgttgc
     8221 tggctggcat tgatgaaatc aacaatggct tccactttgg gttgcatact gccgacacca
     8281 aactgacctt cccgcgacag cgtttgcgct tcggcaacgc tgatagcgtc gagccactgt
     8341 tgttgtggtg taccaaagtt aatcgccatt ttttctacac cggtggggat caccagcaga
     8401 tctgcgccta actgactggc gagcagcgcg gaggccagat ctttatcgat cacggcttct
     8461 acacctttca gttgttgccc gtctcgcacc accggaatac cgccgccgcc gcaggcaata
     8521 accagatatc cctggcgcac caggtgagcg atggtgtcgt gctcaataat ttccagtggt
     8581 gcaggagagg gaactgtacg ccgccaacca cgcccggcgt cctccatcag cgtccagccg
     8641 agttgttgtt ggcgttgttg ggccgttgct tcatcgagaa acgcgccaat tggtttgctg
     8701 gggctggcga aagcatcgtc atgtgggctg actcgcgttt gtgtcaccag ggcaattacc
     8761 ggtttgttta tgccacggcg agccaattcg ttatgcagcg ctttctggaa catgtagcca
     8821 attgcacctt gtgtatcacc cacagcgtaa tcaagtggaa ccggagaaac ttcgttactg
     8881 gcgagttcag agcggcgcag aataaagccc acctgcgggc cattaccgtg ggttagcacc
     8941 aggtcccatc cggcttcaac catatcaacg atatgttgca cgctttccat cactgcaaca
     9001 tattgatcgg gaatactatt gcgttgctcg tcctgaatga gcgcattgcc gccaaccgcg
     9061 acgacagcca gtggcttaac catgatggtt ctcctttacg acgatctccg ccagtgcacg
     9121 tacagcggcg acaaaacagg ccatcggcgc agtggtgatc ccggcaccaa tttgcccgac
     9181 ccccgcttgt ttgtgggcga tacccgtatt gatgaccggt aaaatgccgt gatcggtcac
     9241 tttacgcgca tcaatccctg ctgcggtggg ggcaaaattg agggctggaa gtgtaaatgc
     9301 ggggttaccg ccgagggtga tggcttgcat ccgacggctg ttattggtgg catcggcagg
     9361 ggtgcctccc acaaatttga cgatcgccgg agacgacgcc atcgcgaagc cacccacacc
     9421 tgcggtttcg gtaattgcgc tatcacccag gtcagcagca gcgtcgtcaa cgccaaatcc
     9481 ggggaagaac agaccttcaa cggcattggc aggagcctgg aaccagcgat cgccagtacc
     9541 ggaaagccga ataccaaaat tcaccccgtt acgcgccatc accgtaacca ttgaactgaa
     9601 cggtacattg gcggcggcat ccatcattgc tttacaggca gccatcgaca gattgaggaa
     9661 gaagtgatcg ttaccggtga taaatgccac gacgcgctgg atatgttcct gtggaagaga
     9721 gcaggccagc aatgccggga gcaggcgctt aatcagcaaa ccggtagcgg cagcattgcg
     9781 gttatgaact tcatccccca tatgcagcgc ctgcgccatt aatggcttga gttccagctc
     9841 gccgtgttgt gcgattgctg ctttcattgc cggcgccagt tcatcgcgca tccaggccag
     9901 acgattgaga acttcatcgt tattggcgcc aaaacggaga accttaccca gaccttcatt
     9961 gaaattgcta aaagtacgat ggccgttggt tttgttttct accacccata acggcatcga
    10021 cgggctgatg atgcctgcca ttgggccaac cgcgtgataa tgatgacaag gggcaaggtc
    10081 gatttcgcct gcgtttatct ggttttcagc gtcttgctgg ctggtggccc aaccttcgta
    10141 cagcatggca ccaataatcg ccccttttac cggaccacac atctcttccc aggcaatcgg
    10201 cgggccagag tgcaaaatac gtttttgttc agccattgct ggaatggcct ctttagcgag
    10261 catgacatct accagcacgg gctgtgcttc gagataacgt gtcatggcga tttggttggc
    10321 attttctacc agtggatgac gtagcaacga tgccagatcc aatccagcgt caatatcgcc
    10381 ttgtgctggt ggctgccagt tgagtgcaat tgcgcttccc ccggcatgtt gaatattgtc
    10441 agcaaaactg tgtagtcccg cgttcacgac cttcaaaggc tgattaaata aggtattcat
    10501 acgtttagcc cctttttctg caactgagtc tgtgcaacgg tgctggccca tagcgccgcc
    10561 tgtgcattgc tgctggcgat gatgacccct gcattttgta gtgcgcttat ctgctgactg
    10621 cgtatttgcg gatcagcttc cgttccgcaa acgtgcgcaa tcagcaacgg tgcgttattc
    10681 atgtcgatgt gtgaaagttg gtccagtaat tcgctggcag gcgtcgtgga agcgccataa
    10741 ccaagtacca ggtcgaataa cacgacggca gtgtgtgagt cgttgagttc gttgagcaga
    10801 cgttgattgc gcagagttgg gtcgatcatt ggatgcggtt tgccacgggt gaaatcatcg
    10861 tcgcccatat cgatcaacgt gtgatcttcg ctttgccaga tatttgccag agcacgatta
    10921 cccgcaactg gcgtattgga agctgcgctg aatccctttt gttggcagat aagttgcgct
    10981 tcgtagcaga aggttcctcc ggcgaaaaca ccgcgtatcg cctggcgatg ggctggcagg
    11041 cgctggcagg cgttttgtaa cattgtgaga tcgtcgcagg gtacatcggt ttcgacagcc
    11101 ggaagtggct gagcattcag taacgccaca gcgatgtcgg cggcgctggc aagtgtggtg
    11161 gcggcagtga tattgggacg tgccagatca tgcggattcg ccccgaggaa attcaccact
    11221 acgggttttc cacaagcctc ggcacgctcc agaatagtcc gggcaacgat cggtgaaggc
    11281 ggttttgata taagcacaat cacgcgagtt tcatcgtctt gcgccagcgc atctaaagcg
    11341 aagagcatcg atattccacc aatctcttca ctgagatcat gtccgcctgt tcccaacgcc
    11401 tgagaaatac ccgctccgag ctggtcgatt cggcaagtca cttcctgtag cccggtgcct
    11461 gacgcgccaa taacaccaat tgctccgcgt ttcaccacat tagcgaagcc taacggaatg
    11521 ccgttaacaa tggcggttcc acagtcaggt cccattacga tgcgctgacg ttctcgggca
    11581 agcgttttga tggatttttc ctgcccgata ctgacgttat cgctgaacat catcacgttc
    11641 attccgaggt ttaacgcttt aattgcttcg gcggcggcgt agtctcccgg tacagaaatg
    11701 agtgccaggt tggcttcggg ttcggcttcc agtgccattt ccaggctgac cttttccggt
    11761 gttttaatgc cactatccgt ttcatcatcg ggtttactgg tcagacgttg ctgggccagt
    11821 gccagtgctt cattgcaaat atcttcttca cccatgactg caatcactaa atcattggga
    11881 ctggcgttga tttcgttacc cagacccgca tcgcgcaatt gttcgagatt attgggcgtt
    11941 cccatgacga ccgaggcctg ttgaataccg ggcaacttgc tgatttgcgc ggaaatctgc
    12001 atcagagaaa ctgagtcctg ataaagatta ctgaaaactt tatatttaac tttcatgctg
    12061 ttacgcctgt ttcattccgc ccgatagcaa aattcgggca aagcatcccc gtgtagcccc
    12121 ataaacgggc tacgggagaa aaggttgaaa tcaattcatg gtgttcaggg ttcgaaagcc
    12181 atgcagcatt cctgcaaggc aatctactcc tgacgttgcg ccaaattgca tgacctgttc
    12241 tgcacaggat gcgattgtca ttgccgatgc gctcccaacc agttgtgcga gtaactggca
    12301 aatcggttct gaaaaatgtc cctgaagcgc acgctccaga taatgacgac tgatatccgt
    12361 ggtgttgtgc gcatgttgat caattgcctg ctgcaaccgg tattgatgat cggcaagtgg
    12421 tgcagggagt tgccataacc agagtgctgc cagatagccc aacaaatagt catctccatc
    12481 gggcgttaag cctttaccaa agccgatcag ttgtggcgcc tggcgttcca gcgcatctgg
    12541 atgttccata atcgctaatc cctggtgcgt gagactatcc ggcaggtgca atgtgctttg
    12601 aacgttatgc tccagacaaa ataaaagccg ttgcgatgcc atcgttgggt agtgctggaa
    12661 aaccaaaggg aatgcctcaa gtgtcagcat tctccccaca aatggcgact gccagagcgt
    12721 tgcgttatcg agcgccacac aaaatcgagg tgtccgcagc aagcctgggg ttaactgaat
    12781 gggatctgaa tgggcaatct ctcttcgcca gtcccaacat tcaggtagcc agactctggc
    12841 agcatcaggg agattcagat acgtttgcgc acacaacaga gtgatgaatg ttcccgcgtc
    12901 tgaatacaga tttaccgcat gtttaaaagc gctatgcaga cgtaaatctt gcgtttcatt
    12961 ctgcgggaaa agatatccca acgacgatgc gcaaagcgta acctgtacca tcggcgttag
    13021 ccttccagac ctgcaataat ggctttggaa tcagtaaccc aaccgaaaat ggcaccttgt
    13081 gctttgatca tctcgagggc gtatttctgg aattccggga aataggagcc gacacaatct
    13141 tccgggataa tgcattcata gccacgatca tttgcttcgc gcacggtggt ggttacgcaa
    13201 acttcggttg ttacaccaca aactatcagc gtcttgatac cgtggttttg caaaatcaga
    13261 tgtaagtcgg tttgataaaa cgcgcctttg ccaggcttat caatcaccgg ttcgcctgcc
    13321 accgggtaca gttcaggaat gatgtcatga cctgcttcac ctctgaccag aatgcgtccc
    13381 atcggtcctg gttcaccaat aaaggttttt ccgccgcgcg tcagtttggc tggcgggcag
    13441 tcgcttaagt ctgcacgatg gccttcacga gtatgaataa ccatagttcc tttttgacgt
    13501 gcggctgcca gcacctctgt acatggtgcg atagccgtgc gtaccagtga aacatcattg
    13561 cccagagcct caccgaagcc ccctgcttcg acaaagtcac gctgcatgtc gatcatgacc
    13621 agtgcagttg tgcgcggatc aaaaggtaat tcaaagggtt gagcctgaaa aatggattgc
    13681 gtcatggtaa atctcctgtc tgatgtttaa acgatccagt tgttctgaga acgagtattc
    13741 aggagaaaag cgacacaaaa caggaaaaaa gtttgccgca cagcaacgaa atttttgcat
    13801 tcagcaggtg gtgtgatgac gaatgtgaag ggagtcaaag agtggcatac agcaacaaat
    13861 tttttgatgc tgtatggttt tgagattaaa aaaatcgttg aaattgtgtg agcttaatca
    13921 tcagatttag cgactatggc gttacttttt aacaccatac tgtgtaaacc attccaacat
    13981 tcgctgccag ccgtctttcg cagattcggc atgatagctc gggcgataat cggcgttgaa
    14041 cgcatgccct gcatccgggt acacgataat ctctgctttc gcattagcag cccgcagcgc
    14101 ctggcgcatg gtttcaacgc tctcctgcgg aatgctgtta tcctgaccac catataagcc
    14161 gagaaccgga gcgttaagat cggttgcgat atcaacaggt tgtttcggtg aattcagcga
    14221 cttatcgccc gtcagtttgc cgtaccacgc cactgcggct tttagctgtg gattatgcgc
    14281 ggcatacagc caggtgatac gtccacccca gcagaatccg gtgatcatta aacgatgaac
    14341 atcgccgccg ttgcgggaag cccaactggc gacatgatcg agatcggcca gcacctgcga
    14401 gtcaggcact tttgctacca gaccgctaag caacgtgggg atatcggcaa aatcattcgg
    14461 atcgccttcg cggaagtaaa gttcaggtgc gatagccaga tacccctcca gcgccagacg
    14521 gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt tcctgcacta caatgaccac
    14581 tggcagtggg ccatcgcttt gctttggtct ggcatggtaa gcaggcatgt tatccccttg
    14641 tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc ggagtctgaa cgatggtcga
    14701 agcgagaggc gatgcagcag gtgcaaatcc agattgttgt gttgttgcca tggtattctc
    14761 cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc agcatgcaac aaatcacatt
    14821 gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga agagtgattt atgtgatttg
    14881 catcactttt ggcgggtaaa tttatgcaac gcattttcgt catggtgatg agtatcacga
    14941 aaaaatgtta aacccttcgg taaagtgtct ttttgcttct tctgactaaa ccgattcaca
    15001 gaggagttgt atatgtccaa gtctgatgtt tttcatctcg gcctcactaa aaacgattta
    15061 caaggggcta cgcttgccat cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg
    15121 ctgatggata agccggttaa gctggcatct caccgcgaat tcaccacctg gcgtgcagag
    15181 ctggatggta aacctgttat cgtctgctct accggtatcg gcggcccgtc tacctctatt
    15241 gctgttgaag agctggcaca gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc
    15301 gctattcagc cgcatattaa tgtgggtgat gtcctggtta ccacggcgtc tgttcgtctg
    15361 gatggtgcga gcctgcactt cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt
    15421 acgactgcgc tggttgaagc tgcgaaatcc attggcgcga cgactcacgt tggcgtgaca
    15481 gcttcttctg ataccttcta cccaggtcag gaacgttacg atacttactc tggtcgtgtt
    15541 gtgcgccact ttaaaggctc gatggaagag tggcaggcga tgggtgtaat gaactatgaa
    15601 atggaatctg caaccctgct gaccatgtgt gcaagtcagg gcctgcgcgc gggtatggtg
    15661 gcgggcgtta tcgttaaccg cacccagcaa gagatcccga atgctgagac gatgaaacaa
    15721 accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc gtcgtctgct gaaataaaaa
    15781 aacgctcccc aacttcgggg agcgatatca gttattgacg ccgtaaacgc attattttgc
    15841 ggattattgg cgtcagtagc gtaaaggcgg ccagagtcag caatgccaga gtgatcggtc
    15901 gggtatacag aaagtcgagt gacccaccag aaagcgacag cgcacggcga aaattacttt
    15961 ccgccattgg ccccataatc aatgccagca ctacgggtga tgccggatat cctcctttct
    16021 gcatgaaata gcccaccacg cctgcaatca gcataatgcc aacatcaaaa aggctattgt
    16081 tcaacgcata agtcccgacc acacaaagtg cgaggatcat cggtgtgaga atattgccgg
    16141 gaattttcac cacattaccg atgactttca aagagagcag tccaagaact agtagcatga
    16201 agtaacagaa gatcatgccg acaaacagcg tataaaccat atcaccgtgg tcggtaaaca
    16261 tcagcgggcc tggttgcatt ccctgtaacg ttaacgcgcc cagcatgatg gcggttaccg
    16321 catcgccagg gatccccagc gtcagcatag gtaacaatgc gccaccggtg cagccattcg
    16381 cgcctgcctc acaggaggcc acagctttaa tttcaccctt accaaagttt tctggtgtct
    16441 tactgaaacg acgagtttcg ttataggcaa caaaagctgc aatatccgcc cccgcaccgg
    16501 ggatcatgcc gataaaaacc cctaatcctg atgagcgcaa aatggttaat gcaatgcggc
    16561 gtagtgtttg ccagggcagt aacagactgc cgatagccac cttcgcgccc tgacgaattt
    16621 gctggttttg ctccatagag cgaaatgctt ctgaagcagc aaataaacca atcatcaccg
    16681 ggataaatgg cacattcatc agctccacaa atccacctgt aaaccgtgga aaaccgccca
    16741 ttggatcaag gcctatcgtt gcgatcagca atccgccaac cccggcaatc agccctttaa
    16801 tcagtgactc accggaaata ctggcaataa tgctcagtcc gaaggttgcc agggcgaagg
    16861 actccgaggc actaaatttc agcgcccaac tggcaagtac cggagagaga aacatcagca
    16921 cgataatgct gagcgttccg ccactaaagg aagagaacgt ggcggcactc aatgccagcc
    16981 ctgcttttcc ctgtttcgtc agctcataac catcaatagc ggtggctgcg gcggcaggcg
    17041 ttccgggaat tttgagcaaa attgcggtga tagaaccacc gtagacgcca ccaaaaaaga
    17101 cgccgcaaat catcaacaag ccagaaacag gctccatgcc ataggtgaaa ggcagcaaaa
    17161 tcgccacacc catggtcgct gtcagaccag gcaaagcgcc aatcacaata cccagcatca
    17221 ccccaaaagt ggcaaacagc agcgcctgag gattattgag taatgtcgaa aaaccctgag
    17281 ttaaaagttc actttcaaac attgctggcc cctgttattc gaaaaatgtg ccgacaggca
    17341 atggcacctg taacgcttcg acaaacagca accagaggat tgccgttaac agcagcgcaa
    17401 tacccggtgt gagtacccat cgcctgtagc ccatcaacgt cataatgacg atgaggaaaa
    17461 caggagtagt aatgagataa ccgcagtagc tcatcgccac aatataaaac gcggttgccg
    17521 caattcctgt tgctgttttc ctgaatgccg gtttttcctg tgcgttggcg caagaggctg
    17581 gttttgcctg caaaagatcg cgtatcacca gcaagacagc gagcacaatc agggcacagg
    17641 catagaaggt agggaaggca cttgcaccgg gatccgtttc aatggcagtt gctggaaaat
    17701 cagcggcatt aatcatcacc gcgccagcaa ccacacaaag taacagcgcc accaccatcg
    17761 ttgatgaaag gcgcttgcgg tgcggtacgg cgagttgagc aacagactgt tttgtgttca
    17821 tcaaccgtac ctcaggttta ttttttcagg ctcaggcgag tcagcaactc gtcaaagtac
    17881 ttttcctgtt cgctgatttg ggtctggaag ctggcagcgt caagccatgc atagttgaga
    17941 ttcaactttt gcagtgcatc ctggaatgca ggctcttcag ccgtctcttt tgctgccttt
    18001 gccagaacat ccaccacatc ctgcggcgtt ttttgcgaca caatcaggcc gcgccaggtg
    18061 ccgatggaga gatcaacgcc tttctctttt aacgtcggga cgtcaggcat ggttttcatt
    18121 cgctcatccg ccatcactac cagtgtcttc agcttgccgc cattcacatg gttgataact
    18181 tctcctgggc ttacggaaac cgcttcaatg tgcccaccta acaggcctgt aatggcaggg
    18241 gctgcgccgt catacgggac atgagaaaac tttgcgcccg ttttgtcttc cagtgcagct
    18301 gccgccagat gccagatagc tccggtgcct gagttaccaa tgcgtacttt tccgggattc
    18361 gctttggcgt aagtcataaa ttcttcatag ctattccacg gcgcatcggc acggactgtg
    18421 atagcagccg gatccgcatt cagacgggca atgggtttaa agtcgctggt tttaaaacgc
    18481 accattccga ggctgggaag ggtggttagt tcaaccgtgc ctaaaccaat tttgtaaccg
    18541 ttagggcggg cagcggcgat ttcactcagg ccgatagcac cgcctccgcc aggtttattg
    18601 ataaccccga tgctgacggg taaatggttt ttggcggcat cagcaaaggc acgggcaacc
    18661 agatccgtac cgcctccggc agcgtaggga acgactaact cgatctgttt ggttgggtag
    18721 gaggcagcct gtacaggcgc aatgctgagg gcagcgcctg caagaagtac cattgcagcc
    18781 atttttttcg gtaaggtaag atgttttatc attgctttta tatccttatt tatcagttag
    18841 ttgctattta aattgttcca tgcgttcata aacagtactg cattttcata gacttgctct
    18901 ggcgtcatgc cggggcgata tagcgcagaa ccgaggccaa agccgtttgc gccgtggctc
    18961 agataacgcg ccatggtttc cggtgtgata ccgccaaccg gtaacatcgg aacggattgt
    19021 ggaataaccg cacgccaggc tttggtaact tctggcgtaa tttgttcagc cgggaacagc
    19081 ttgatggcat tagcaccatg ttcaatggca gcaaaggctt cggtgggcgt ggcgacgcca
    19141 ggcgcacaat acatacccag cgcacgggcg cgacggatca ccgcagtatc gcaatgcggc
    19201 atcacgataa tctggccccc agcctcttta accgcaacca cctgctctac cgaaagcaca
    19261 gttccggctc cgaccaggca gtccttaggc agggcgtcgc gcagtaaact gatactttgc
    19321 aacgcctccg gcgagttcat aggaatctca atcaggcgga atccggcggc gtacagtttt
    19381 tcaccgatgg cgacaatctc atccggtttc acgccgcgta aaatggcgac taacccggtt
    19441 ttttgcaaaa gcgtattaaa tggcatgggt acattccctg gcgttagtgg acagtaaccc
    19501 ggcagcgtgg gcgattcgcc ataatccatg ttcagtggca ttgggaatgg cgtgtatctg
    19561 tctgtcggga aaaacgtggc tgaatgcttg tgaatagcgg ctgttcagat ttgcgctacc
    19621 gattaaggcg atcggtgttg tggcgggtaa atcacgtagc aaactggatg ccagactaca
    19681 taattcatgg cctatcagta atccggaaag gtaatccccc tgcgaggacg acgtaagatt
    19741 actggtcagc aggcgcgaac gggtcgaaaa cagcgtcgat aataatccgg tctgatgatg
    19801 ctcttgtgct gctgtcagcc cgtgggtgaa ggcatcccag tgcggttcat ctccaggatg
    19861 catggtggca ccgagaatcg aatggcggga taaaacatca aataactcgc ccgtcataaa
    19921 ggttcggaaa tcggtaatgg tgttgttttc aactactgcc catttcgcat gtgtgccggg
    19981 taagcctatc agcactggca taccctgatg gatcgcgttt tgcagggccg gttccatcga
    20041 gatagcaccg aagatttgtg tctcttcgcc acgcatcact tcgggcattt caccttcttt
    20101 aatcacgcca ggaatgatgt gcagcattgt ctgctgctgg tggataacag gcgtcaactg
    20161 cttcgccaga ccctcgagag tgaccgggca aggtaaataa gctgctgatt gccacccttg
    20221 cgcactgccg agcatgccgc aagccagtac cgggcatggg gtttgtgcca gccagtcgcc
    20281 gcagacacgt aaaaacgtat cgtggaaagc gtcctcacgt gttgctgcct gggagggtaa
    20341 tttcatgatc ccccagggga attcacgttt ttcttgtgga tttggtgatt cgccaaaacg
    20401 ccatgcccga agcgatgacg ttccccagtc cagggcaata aatgagggtg ctcccggcat
    20461 tagtcgtttc ctttaatcat ggtttattta tctttgcgga cgtgcttacc ccagcccagt
    20521 tcagcggata tctcttctgc acaggatttg acgtaaggtg ccagttcttc cagacgcgcc
    20581 agcgacatat acgtggtggt actggcgaca gaaatagccg ccacaatttc atcccgggcg
    20641 ttgtaaaccg gcgctgcaac gcaacggata gtcggctcgt tctcttccag atcaaaagca
    20701 aaaccactgg cggcataacg acgcatgttc tgaataaatg tgcccagttt tgtttcatca
    20761 ccacaggtct taaacagtgt gcgccactct tcttctgttc ggttgaggat caatgccttg
    20821 ccgatgccgg tgattgccag tggcatgcga tggcctgggc gtgagcgcat ttccagcccg
    20881 cgctgactgt tgatcttctc cagatacagc acttcgtcgc cttcaacgat ccccagatgc
    20941 acggtgtcca gggtgtaacg ggcaaggcgt tgcaaaacgg gctgggcaat ttcgtacagc
    21001 gaggtgcttt ccagtgctct ggcaccaaat tcgatcagtt tggcacccag ttggtagccc
    21061 tgcgttggga catggcgcag gtagcgttgt tccaccagaa cggtgactaa acgatgggtt
    21121 gtgctccggg tcgtgccggt gaactcgccg atacttttta ggtcatggca gccgttataa
    21181 acagcattaa gaaccgccag tccacgtaat aacgtttgtg cgcctgcggt ggttgaagag
    21241 gtttctgcca tacgtttctc ctgatgatat tgctgaagtg ttccacataa tgacgtgggg
    21301 aaaaggtgat atatcccaca atataaactc catctcatat tgtgggataa aataatgaaa
    21361 attaacgatc gttttgcgta gcgcctcgca ggaaacttct ttcaaactgg acgtttgtac
    21421 agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa
    21481 tcatggttta cgcagttatt gcattggtgg gtgtggcaat tggctggctg tttgccagtt
    21541 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
    21601 taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgcgcagag tgtgaattgc
    21661 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
    21721 aagtgaccac gcggatggaa gccgcacagc aacacgctga cgataaaatt cgccagatga
    21781 tcaacagcga acagcgcctc agtgagcaat ttgaaaacct tgccaaccgt atttttgaac
    21841 acagtaatcg tcgggttgat gagcaaaatc gccagagtct gaacagtctt ttgtcgccgt
    21901 tgcgtgaaca actggatggt ttccgccgtc aggtccagga cagctttggt aaagaagccc
    21961 aagaacgtca taccctgact cacgaaatcc gcaacctcca gcaactcaac gcccagatgg
    22021 cccaggaagc gatcaacctg acgcgtgcgc tgaaaggcga caataaaacc cagggcaact
    22081 ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
    22141 atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
    22201 tgcggttgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
    22261 aacgctattt caacgccgaa gatgactaca cccgcgaaag cgcgctacag gaacatatcg
    22321 cgtcggtacg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
    22381 tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
    22441 ttgaccgtca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
    22501 cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat gagcatcaaa
    22561 gtcgtaacgc ccagaaaatc gccgatcgcg ccagcaagct gtacgacaaa atgcgcttgt
    22621 ttgtcgatga catgtccgcg atcggtcaaa gtctcgacaa agcgcaggat aactatcgcc
    22681 aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc
    22741 gcggtttagg ggtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
    22801 gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
    22861 aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggggtagt taagccgggt
    22921 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
    22981 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
    23041 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
    23101 gtcatgaatg atttgatgtc attcggtatt catcgtttgt ggaagcgatt cacgattgat
    23161 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
    23221 acagcgaaat tctctcgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
    23281 gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
    23341 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
    23401 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
    23461 cgcgtgctga aacccggcgg ccgcctgctg gtacttgagt tctcgaagcc aattatcgag
    23521 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
    23581 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
    23641 caggataccc tgaaaaccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
    23701 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
    23761 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
    23821 ctcacccgcg ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc gcgtggaggt
    23881 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggtcg atgtactggg
    23941 cgaatgggca ggcgatgctg actgcaccgt tatcgcttac gccagcgtgt tgccgaaact
    24001 tcgtgatcgc cagcagcttg ccgcactgat tcgcagtggt gagctggaag tgcagggtga
    24061 tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga
    24121 actgctggcc ccttataccg gtgatatcgc cgctgaaggt atcagcaaag ccctgcgcgg
    24181 aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa tgttatgtgg cggaagccat
    24241 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
    24301 ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa
    24361 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
    24421 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
    24481 ttattctgga tgccaaatcg gcataaagac aaacctttag gtgagcgact acgactggcc
    24541 ctgcaagaac tgggaccggt atggatcaag ttcgggcaga tgttatcaac ccgccgcgat
    24601 ctttttccgc cgcatattgc cgatcagctg gcgttattgc aggacaaagt cgctccgttt
    24661 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
    24721 tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc
    24781 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag ttatccgccc ggatattttg
    24841 ccggtcatta aagcggatct gaaacttatt taccgtctgg ctcgctgggt gccgcgtttg
    24901 ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg
    24961 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg acgcaatttt
    25021 gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg
    25081 atggtgatgg aacgtattta cggcattccg gtgtctgatg ttgcgacgct ggagaaaaac
    25141 ggcaccaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt
    25201 cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac
    25261 ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
    25321 gataaacgct atctggcgga aaactttatc gccttcttta atcgcgacta tcgcaaagtg
    25381 gcagagctac acgtcgattc tggttgggtg ccaccagata ccaacgttga agagttcgaa
    25441 tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt
    25501 ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg
    25561 caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat
    25621 ccgcaactcg atttatggaa aacggcgaag cctttcctgg agtcgtggat taaagatcag
    25681 gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
    25741 atgccagaac tgcccgaact ggtttacgac agtttgcgcc agggcaagta tttacagcat
    25801 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
    25861 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
    25921 cctgaatggg ggttgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
    25981 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg
    26041 cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
    26101 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
    26161 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
    26221 ccaaagcagg ataaaaccag ccaggatgct gattttactg cgaaaactat cgccgataag
    26281 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
    26341 caggtgtaat ctgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc
    26401 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt
    26461 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
    26521 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc
    26581 gaactgaaag cgtcgatgga tgaattacgc caggctgcgg agtcgatgaa gcgttcctac
    26641 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg
    26701 aaagacaatg aaactgcgca tgaaggcgta acgcctgctg ctgcacaaac gcaggccagt
    26761 tcgccggaac agaagccaga aactacgcca gagccggtgg taaaacctgc tgcggacgct
    26821 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
    26881 aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca
    26941 ttatctcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
    27001 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
    27061 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
    27121 cgccggtgat tctctatcag gtgtgggcgt ttatcgcccc agcgctgtat aagcatgaac
    27181 gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcgt
    27241 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
    27301 gggtacaggt atccaccgac attgcgagct atttaagctt cgttatggcg ctgtttatgg
    27361 cgtttggtgt ctcctttgaa gtgccggtgg caattgtgtt gctgtgctgg atggggatta
    27421 cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg
    27481 ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc cctatgtact
    27541 gcctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaaccggg
    27601 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
    27661 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
    27721 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
    27781 gctactcatc accggtacca atctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
    27841 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
    27901 tgtgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga
    27961 atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac tcgcttttgt
    28021 tgcccagcta cgcattgccg cagaattaaa catgccggta tttatgcact gtcgcgatgc
    28081 ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct
    28141 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcgtg gaatttatat
    28201 cggcattacc ggttgggttt gcgatgaacg acgcgggctg gagctgcggg aattgttgcc
    28261 gttgattccg gcggagaaat tgctgatcga aactgatgcg ccgtatctgc tccctcgcga
    28321 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
    28381 acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgccaa
    28441 tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
    28501 gtgcttaatc tctttattaa taagattcag caatagcatg gagcgagcct cgccatcggg
    28561 ctcggtgaaa atggcctgaa aaccttcgaa tgcgccttca gtaataatca ctttatcacc
    28621 aggatacggc gtcgatggat cgacaatatc tttcggttta tacactgata actgatgaat
    28681 caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccacg
    28741 agtcgcgttg atagtcgtgg tatgaatcac ttccggatcg aactccacaa acaagtagtt
    28801 ggggaacaat ggctcactga ctgcagtacg tttgccacgc acgatttttt ccagggtgat
    28861 catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg
    28921 cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg
    28981 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
    29041 cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
    29101 gggcgcagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt
    29161 gacgctgctt gaacagcagg gtgagctaaa acgtgtcacg ctcccggtgg atccgcacct
    29221 ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga
    29281 aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt
    29341 ggcgatgggc atggggcagg aggatgtttc ggcgctgcgt gaagttggta aattattggc
    29401 gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca
    29461 gtttaagcaa gtattgaaca tgccgacaaa gcgactgcgt ggtgcgccct gccaacaaaa
    29521 aatcgtctct ggcgatgacg tcgatctcaa tcgtattccc attatgacct gctggtcgga
    29581 agatgccgcg ccgctgatta cctgggggct caccgtaacg cgcggcccgc ataaagagcg
    29641 gcagaatctg ggcatttatc gccagcagct aattggtaaa aacaaactga ttatgcgctg
    29701 gctgtcgcat cgcggcggcg cgttggatta tcaggagtgg tgtgcggcgc atccgggcga
    29761 acgtttcccg gtttctgtgg cgctgggtgc cgatcctgcc acgattctcg gtgcagtcac
    29821 ccccgttccg gatacgcttt cagagtatgc gtttgccgga ttgctgcgcg gcaccaagac
    29881 cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct
    29941 ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg accacaccgg
    30001 ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga
    30061 agatgcgatt tatcattcca cctataccgg gcgtccgcca gatgaacctg cggtactggg
    30121 tgtagcactg aacgaagtgt tcgtgccgat tctgcaaaaa cagttcccgg aaattgtcga
    30181 tttttacctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga tcaaaaaaca
    30241 gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    30301 gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt
    30361 gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg tagaaaatac
    30421 gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct
    30481 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    30541 agatccagat gttgtcgcgc atattgacgc catttgggat gaactggcta tttttaacaa
    30601 cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg
    30661 catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg
    30721 tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt
    30781 gatggatgag cgcgacaaac gtccgttctc aatggcttcg acgccagatg aaaaagggtt
    30841 tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg
    30901 catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga
    30961 tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc
    31021 gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg
    31081 gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt tgaagcatcc
    31141 tggtctacaa gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg
    31201 caccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg atatctatat
    31261 tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc
    31321 gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaacccgcc
    31381 cctgacaggc gggaagaacg gcaactaaaa ctttatacag tggcattact ctacgttgca
    31441 tccggcaaac agtgcccgat gcaacgctgc cgcgtcttat cgggcctaca aaagttctga
    31501 accgtgggcc ggataaggcg ttcacgccgt atccggcata cagtgcccga tgcgacgctg
    31561 ccgcgtctta tcgggcctac aaaagttctg aaccgtagac cggataaggc gttcacgccg
    31621 catccggcaa gtggttaaac ccgctcaaac accgttgcaa tgccctgacc cagaccgata
    31681 cacatcgtcg ccagaccaaa ctggacgtct ttgtgatcca tcagattcag cagcgtggtg
    31741 ctgatacgcg caccggaaca acccagcgga tgacccagcg cgatcgcgcc accgttgagg
    31801 ttgatcttct cgtcaatctg ctccattagc ccgagatctt taatacacgg caggatctgc
    31861 gcggcaaagg cttcgttcat ctcgaatacg ccgatatcgc tggcagaaag ccccgctttt
    31921 ttcagcgcca gcttcgaggc cggaaccgga ccataaccca taatcgatgg gtcacaacca
    31981 acgaccgcca tcgaacgcac gcgggcgcgc ggcttaagac ctaattcacg ggcgcggctt
    32041 tcactcatca ccagcatggc agctgcgcca tcggaaagcg cagaagatgt acccgccgtt
    32101 accgtaccgc tgacgggatc aaacgccgga cgcagtgtgg tgagggcttc cacggtggtt
    32161 tccgggcgaa tcacttcgtc gtaattaaac tgcttcaata cgccgtcggc atcgtgacca
    32221 ctggtcggga tgatttcatt tttaaatgcg cctgactgcg tggcggccca cgcgcgggcg
    32281 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
    32341 atttctgccg ttaagcccat catgcccgcc gctttggcga cattacggct aaggccgggg
    32401 tgaaaatcga cgccgtgact cattggcaca tggcccatat gctccacgcc gccaaccaga
    32461 catgcctgcg catcgccggt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat
    32521 gaaccacaca agcgattaac ggtaaccgcc gggacagagt gcggcacttc agccaacagt
    32581 gccgcgttac gggcgatatt aaaaccctgc tccagtgttt gctgcacaca accccagtaa
    32641 atatcgtcaa gggccgccgc ttccagtgcc gggttacgcg ccagcaggct acgcattaaa
    32701 tgagcggaga gatcttccgc acgtacgtta cgaaaagcac cgcccttcga acggcccatc
    32761 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
    32821 aggtcgccaa ccggacgggc tggctcaacc gggggatagt actgttcgtt atgacgtgct
    32881 ttattacgca gaccttccgg tacttcatac agcgggccga ggtgctgata ttgctgtgcc
    32941 atatcgaggt attttgcgct gccgagggtg tccagccagc ggaacgcgcc gccgtggaac
    33001 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ctgccggagt ggcgataatg
    33061 ccctcttcca gacagcggac cacttcgttg accatcggga tcatcatgcg agcgataatc
    33121 tcttcttcgc tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc gtcaacagcg
    33181 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
    33241 ccgttcttct gaccaaagcg gttggcatta aacagcgcgt cgatggcatc gcgataatct
    33301 ttctgcatcc gctgcgggaa gcctgctgcc atgactgcct gggcgtgatg cgcggtgtca
    33361 atgcccacaa catccagcag atatgccgga cccatcggcc agccaaactg tttttccatc
    33421 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca actggctgaa accggcgaaa
    33481 taggggaaca gtacacggtt gacgaagaag ccggggcagt cgttaaccac aatcggcgtt
    33541 ttgcccattt tgctcgccca ggcgacgact ttcgcgatgg tttcgtcgga gcttttctcg
    33601 ccgcgaataa tttctactaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
    33661 aagttttccg gacgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
    33721 gacgccagta cggtatccgg gcgtactttc tgctcggttt ctgccagcac ggcttttttc
    33781 actttcgggt tttcaacaac cgcttctacc acaacatcca cgcggtcaaa cccggcgtag
    33841 tcgagcgttg ggtgaattgt ggagatcacg ccagccagtt tcagaccatc gatcttgccg
    33901 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
    33961 tcgttgatat ctttcatgac aaccggcacg cctttccacg cagactggta ggcgatgccg
    34021 ccgcccataa tgcctgcgcc cagcacagcg gcctgtttcg gggtttcaac atctttggtg
    34081 agtttcttcg ctttaccttt tacatattga tcgttaagga aaatgccgac cagtgcgcgg
    34141 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccaggtttaa ggcttcttca
    34201 cgaccaaaac gggccgccgc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
    34261 ttccccgccg tttgcgcgac catcccttta gcgatggtga agctcatggc ggcttcaatc
    34321 ttgctcagtt ttagcggttc cagcttcggc tgacgttttg ctttccagtc gaggtcgccg
    34381 ttaatggcct ggcgtaaaat cgccatcgcc ccttcaacca gtttttctgc tttgactaca
    34441 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
    34501 atttccagcg cactgtcagc accgagcata cgtggcatac gtacagaacc gccaaagccc
    34561 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
    34621 taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg catagccgtt gacggcagca
    34681 atggtcggca ccgggagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
    34741 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
    34801 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
    34861 agcacgccga tggcctcgcc gaggctggcg acggtggcag tgtcgagttt attaactgaa
    34921 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
    34981 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg tgatctggtc gtaccagatg
    35041 catcgaagtg tgtattttgt gttaaaaata tgcaaataaa atattaagga aatgccgatc
    35101 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaatatg cggagactta
    35161 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcgctctat aaaaatcata
    35221 tagctacctt acaggaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcgttac
    35281 ttattcactc cggcgaactg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
    35341 tgaacccgca attcaaagcg tgggtgccag taactcaggt gccaaactgc tggctgctgg
    35401 tggatggcgt gaataagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
    35461 tcgaaccgct gccgaactcc ttctggactg aagatgtaga agtgatcgcg ctgccgaaag
    35521 ccgatggcat tggtagtctg ttgcctgctg cgcgcggcaa tatcggttat atcggtccgg
    35581 tgccagaacg tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg aaaggggtga
    35641 tcgactacct gcattactat cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
    35701 aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga agaagcgttc cgttctggca
    35761 tgagcgagtt cgatatcaac attgcctatc tgaccgcgac cggtcatcgt gataccgacg
    35821 taccttatag caacattgtg gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac
    35881 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
    35941 acggctatgc ggctgatcta acccgtacct ggtcggcaaa aagcgacaac gactacgcac
    36001 agctggtgaa agacgtaaat gatgagcaac tggcgctgat cgccaacatg aaagcaggcg
    36061 tcagctatgt ggattaccat atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
    36121 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
    36181 ttatgccgca cggtatcggc catccgctgg gcctgcaggt gcatgacgta gccggtttta
    36241 tgcaggatga tagcggtacg cacctcgcgg caccggcaaa atatccgtac ctgcgctgca
    36301 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcatcgaat
    36361 cgcttctggc accgtggcgt gaagggcagt tcagcaaaca cttcaactgg cagaaaattg
    36421 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatt cacgaaaaca
    36481 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
    36541 ggcaccggtc atggtcgttg aagagatcaa aaagagccgc ttcattacgc tgttggcgca
    36601 taccgatggc gttgaggcgg caaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
    36661 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg
    36721 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccaactaat
    36781 gggcagcggc gtcggggaaa ttaccgctgt ggtggtgcgc tactacggcg gcatattgct
    36841 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
    36901 aacgacccaa cgcaagacac cattaaccga atatactttg caatgtgaat atagtcagtt
    36961 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
    37021 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
    37081 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
    37141 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
    37201 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
    37261 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc
    37321 tctatgcttt ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt
    37381 ctgatagtgg tgctgttctg gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc
    37441 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
    37501 accacggggg ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat
    37561 cgccagatgc tgcaatggtt tggcggtatg gggatcatcg tgctggcggt tgcgatactg
    37621 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
    37681 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc
    37741 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgctg gaatggatgc ctttgatgcc
    37801 atcggccata gctttgcgac catcgctatt ggcggcttct cgacacatga tgccagtatc
    37861 ggttatttcg acagcccgac tattaacact atcattgcta tcttcctgct gatctctggc
    37921 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
    37981 gatccggaat ttcgcatgtt tatcggcgta cagttttcac tggtggttat ttgtacactc
    38041 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
    38101 caggtggtgt cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
    38161 ctctttttgc cggtactgct cttatgttca gcttttatcg gcggttgtgc cgggtcaacg
    38221 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
    38281 aaacgactgg tgcatccgaa cgccgtctac agcattaagc tggggaatcg cgcactgccg
    38341 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
    38401 agtatgctgg cgattatcgc cacgggcgtg gatgactttt ctgcctttgc ctcggttgtt
    38461 gcaacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
    38521 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
    38581 acattgctgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
    38641 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
    38701 cggaactgaa agaactgggg atccaggcgg atgtcgccaa tgtgcaccgc attgaagaac
    38761 cacagtggga aaactacgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
    38821 actctgcgtt ccaggaattt gtcaaaaagc atgcgacgcg gctgaattcg atgccgagcg
    38881 ccttttactc cgtaaacctg gtggcgcgta aaccggagaa gcgtactcca cagaccaaca
    38941 gctacgcgcg gaagtttctg atgagttcac aatggcgtcc cgatcgctgc gcggtcattg
    39001 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ctttatgatc aaactgatta
    39061 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
    39121 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
    39181 cataaagaat aaaaa
//
LOCUS       DAZFRE010000037        39178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFRE010000037 DAZFRE010000000
VERSION     DAZFRE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.037"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            99..2342
                     /gene="pdeF"
                     /locus_tag="ACHKUI_002246"
     CDS             99..2342
                     /gene="pdeF"
                     /locus_tag="ACHKUI_002246"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6598433.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2381..3922)
                     /gene="ppx"
                     /locus_tag="ACHKUI_002247"
     CDS             complement(2381..3922)
                     /gene="ppx"
                     /locus_tag="ACHKUI_002247"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6598434.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3927..5993)
                     /gene="ppk1"
                     /locus_tag="ACHKUI_002248"
     CDS             complement(3927..5993)
                     /gene="ppk1"
                     /locus_tag="ACHKUI_002248"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6598435.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYELVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6165..6803)
                     /gene="purN"
                     /locus_tag="ACHKUI_002249"
     CDS             complement(6165..6803)
                     /gene="purN"
                     /locus_tag="ACHKUI_002249"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6598436.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6803..7840)
                     /gene="purM"
                     /locus_tag="ACHKUI_002250"
     CDS             complement(6803..7840)
                     /gene="purM"
                     /locus_tag="ACHKUI_002250"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6598437.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8164..8790
                     /gene="upp"
                     /locus_tag="ACHKUI_002251"
     CDS             8164..8790
                     /gene="upp"
                     /locus_tag="ACHKUI_002251"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6598438.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8876..10165
                     /gene="uraA"
                     /locus_tag="ACHKUI_002252"
     CDS             8876..10165
                     /gene="uraA"
                     /locus_tag="ACHKUI_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6598439.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10260..10961
                     /gene="hda"
                     /locus_tag="ACHKUI_002253"
     CDS             10260..10961
                     /gene="hda"
                     /locus_tag="ACHKUI_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6598440.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11099..11458)
                     /gene="arsC"
                     /locus_tag="ACHKUI_002254"
     CDS             complement(11099..11458)
                     /gene="arsC"
                     /locus_tag="ACHKUI_002254"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6598441.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADISLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11479..12942)
                     /gene="bepA"
                     /locus_tag="ACHKUI_002255"
     CDS             complement(11479..12942)
                     /gene="bepA"
                     /locus_tag="ACHKUI_002255"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6598442.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13155..14216
                     /gene="yfgO"
                     /locus_tag="ACHKUI_002256"
     CDS             13155..14216
                     /gene="yfgO"
                     /locus_tag="ACHKUI_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6598443.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14315..14785)
                     /gene="bcp"
                     /locus_tag="ACHKUI_002257"
     CDS             complement(14315..14785)
                     /gene="bcp"
                     /locus_tag="ACHKUI_002257"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6598444.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14785..15357)
                     /gene="gcvR"
                     /locus_tag="ACHKUI_002258"
     CDS             complement(14785..15357)
                     /gene="gcvR"
                     /locus_tag="ACHKUI_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6598445.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15503..16381
                     /gene="dapA"
                     /locus_tag="ACHKUI_002259"
     CDS             15503..16381
                     /gene="dapA"
                     /locus_tag="ACHKUI_002259"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6598446.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16398..17432
                     /gene="bamC"
                     /locus_tag="ACHKUI_002260"
     CDS             16398..17432
                     /gene="bamC"
                     /locus_tag="ACHKUI_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6598447.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWLQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17645..18358
                     /gene="purC"
                     /locus_tag="ACHKUI_002261"
     CDS             17645..18358
                     /gene="purC"
                     /locus_tag="ACHKUI_002261"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6598448.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18526..19389
                     /gene="ypfJ"
                     /locus_tag="ACHKUI_002262"
     CDS             18526..19389
                     /gene="ypfJ"
                     /locus_tag="ACHKUI_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6598449.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19404..21419
                     /gene="tmcA"
                     /locus_tag="ACHKUI_002263"
     CDS             19404..21419
                     /gene="tmcA"
                     /locus_tag="ACHKUI_002263"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6598450.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGLRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21493..22191
                     /gene="ypfH"
                     /locus_tag="ACHKUI_002264"
     CDS             21493..22191
                     /gene="ypfH"
                     /locus_tag="ACHKUI_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6598451.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22272..22472)
                     /gene="ypfN"
                     /locus_tag="ACHKUI_002265"
     CDS             complement(22272..22472)
                     /gene="ypfN"
                     /locus_tag="ACHKUI_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6598452.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22500..23627)
                     /gene="dapE"
                     /locus_tag="ACHKUI_002266"
     CDS             complement(22500..23627)
                     /gene="dapE"
                     /locus_tag="ACHKUI_002266"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6598453.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23631..23987)
                     /gene="yffB"
                     /locus_tag="ACHKUI_002267"
     CDS             complement(23631..23987)
                     /gene="yffB"
                     /locus_tag="ACHKUI_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6598454.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            24368..24427
                     /gene="ypfM"
                     /locus_tag="ACHKUI_002268"
     CDS             24368..24427
                     /gene="ypfM"
                     /locus_tag="ACHKUI_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6598455.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(24526..27639)
                     /gene="acrD"
                     /locus_tag="ACHKUI_002269"
     CDS             complement(24526..27639)
                     /gene="acrD"
                     /locus_tag="ACHKUI_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO6598456.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27803..29503)
                     /gene="narQ"
                     /locus_tag="ACHKUI_002270"
     CDS             complement(27803..29503)
                     /gene="narQ"
                     /locus_tag="ACHKUI_002270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6598457.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29709..31688
                     /gene="aegA"
                     /locus_tag="ACHKUI_002271"
     CDS             29709..31688
                     /gene="aegA"
                     /locus_tag="ACHKUI_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6598458.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31756..32331
                     /gene="nudK"
                     /locus_tag="ACHKUI_002272"
     CDS             31756..32331
                     /gene="nudK"
                     /locus_tag="ACHKUI_002272"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6598459.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            32457..33500
                     /gene="ypfG"
                     /locus_tag="ACHKUI_002273"
     CDS             32457..33500
                     /gene="ypfG"
                     /locus_tag="ACHKUI_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6598460.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(33577..35580)
                     /gene="tkt"
                     /locus_tag="ACHKUI_002274"
     CDS             complement(33577..35580)
                     /gene="tkt"
                     /locus_tag="ACHKUI_002274"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6598461.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35600..36550)
                     /gene="tal"
                     /locus_tag="ACHKUI_002275"
     CDS             complement(35600..36550)
                     /gene="tal"
                     /locus_tag="ACHKUI_002275"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6598462.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36839..39118
                     /gene="maeB"
                     /locus_tag="ACHKUI_002276"
     CDS             36839..39118
                     /gene="maeB"
                     /locus_tag="ACHKUI_002276"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6598463.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT         9266 a         9941 c        10306 g         9665 t
ORIGIN      
        1 ttgctctcta tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc tgtctgaata
       61 cacctccttc aggatgtggg ggattctgct gagcatctat gaaactgaat gcaacttata
      121 taaaaatacg tgataaatgg tgggggcttc cgctgttcct gccttcttta atcttgccca
      181 ttttcgccca cattaatact ttcgcgcata tttcttccgg tgaagttttt ctcttttatc
      241 tgccactggc actgatgatc agcatgatga tgtttttcag ctgggcggca ttgccaggga
      301 tcgccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa acgctctcat
      361 taacggctaa ttttattatc attatcattc tctgttgggg cggttacagg gtttttaccc
      421 cccggcgtaa caacgtttca catggtgata gccgtttaat ttcccagcga ctattctggc
      481 agattgtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgcg tttgtagggt
      541 tactggcgag cagagaaaat ctggtcggcg tcatgccctt taacctcggg accttaatca
      601 attatcaggc cttgctggtg ggtaatctga ttggtgtccc gctgtgctac ttcatcattc
      661 gggtggtgcg aaatccgttt tatttacgta gctattattc gcaattaaaa cagcaggttg
      721 atgccaaagt taccaaaaaa gagttcgcaa tctggctact ggcattaggt gctttactat
      781 tgctgttatg catgccgtta aatgaaaaaa gcacgatttt tagcaccaat tacaccttgt
      841 cattattgct gcccctgatg atgtggggag cgatgcgcta tggttataag ctgatttcat
      901 tgctctgggc ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt
      961 atcctggcta taccacgcag ctcaccataa cctcctccag ttatctggta ttctctttta
     1021 ttgtcaatta catggctgta ctggcaaccc gtcagcgagc ggtagtcaga cgcattcagc
     1081 ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt
     1141 tacgtgatgc cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg
     1201 ttaagaatta tggcatcatg ctgcggattc aatacaagca aaaactttct cactggctgt
     1261 caccattgtt ggaaccgggt gaagatgttt atcagctttc gggtaacgat ctcgcgctgc
     1321 ggctgaatac agaatcgcac caggagcgca ttaccgcact ggatagccat ctcaagcaat
     1381 ttcgtttctt ttgggatgga atgccgatgc aaccgcagat tggcgtcagt tactgctatg
     1441 tgcgctcgcc agtgaatcat atctacctgc tgctgggaga gctaaataca gtggccgaac
     1501 tttccatcgt gaccaacgcc ccggaaaata tgcagcgtcg cggagcaatg tatttgcaac
     1561 gcgaattgaa agataaagtc gcgatgatga atcggctaca gcgggcgctg gaacacaacc
     1621 attttttcct gatggcccag ccgattaccg gtatgcgtgg tgatgtctac catgaaattc
     1681 ttctgcgcat gaaaggtgag aatgatgaac tgatcagccc cgatagcttc ttaccggtcg
     1741 cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt cattgagcat acgctgcaat
     1801 ttatggctga aaacagagcg aagatgcccg ctcaccgttt tgctattaat ctgtctccaa
     1861 cctcggtatg tcaggctcgt tttcctgttg aagtcagtca gctgctggct aaatatcaga
     1921 ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc
     1981 aggcgcaaat caccttgcag catcttcagg aattaggctg ccagattgcg attgatgatt
     2041 tcggcaccgg ctacgccagc tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa
     2101 ttgacggcag ttttatccgc aatattgtgt caaatagtct ggattatcag atagtggcgt
     2161 cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg
     2221 aagagatccg cgaggcggtg ctctctttgg ggatcgatta tatgcagggt tatcttattg
     2281 gtaagccgca accgttaatt gatacgctga atgaaatcga acccattcgc gaaagtgcct
     2341 gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg atttctggtg
     2401 tactttcttc ttcaattttc aaccgccagc cagccacgcc ttcccagtat tcttgctcct
     2461 tttccagatc aagcagtacc agcgcattct gactaaacca gtcatgcggg aaacgcagtg
     2521 tccagtgact gtcatcagta atcagcgtca atgttggcgg tgtggtggtt gcctgacgtt
     2581 gattattgag taatacgcca aggcgcaata gctgaatcag tggcaggaac tgtttcttct
     2641 taaacaaggt aaagcgcggc agatcgtcga gtttaatcgc tttacggtga tagcgcacca
     2701 gtgtcgccat catcagctgc tgttcctgat taaaacccgg caagtcgctg ttttgcagaa
     2761 tataagcgga gtggcgatgc aaaccgctgt ggttgatatt caacccgacc tcatgcagca
     2821 tggcggccca tcgcagtagc gcctccagtt gtggatgcgc cagcttcggt tgctgttccc
     2881 gccactgttc gtacatttgc atagtggtat ccagcacccg tcgggcctgt tcgctgtcga
     2941 tgtgatactg gttggcgagg ctgctggcag tgcgactacg cacatcctga tgacggaaac
     3001 gtccttccat ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac
     3061 ggatggctaa agcatcaaac acaccgcata aaatcgccag tccaggaacg aagactgttt
     3121 tccgctcttc ggaaagacca ggtaaactca gcgatgcgaa attacggtgc cgtaaaactt
     3181 cttttaccag tttttccaga cgttccgggg taattatccc gtctttctcg cccatttcca
     3241 ttaacacttc atgggcggct tttatggtac cggaagcgcc cattgccacg ttccagccct
     3301 gaattcgaaa ttgccaggtt aacgtttcca gtttttgtgc tgccgccatg cgagcgcgct
     3361 gaaaattctc tttattgatg accccgccat gaaaataaag ctgggcaaag ctgacacaac
     3421 ccatccggcg gctttcaacg agaatcggtt cgaaattttc accaattacc agttctgtag
     3481 aaccgccgcc aatatcaata accagtttgc gacctttttc cggttgggta tgttccacgc
     3541 ccataaaaat cagacgggct tcttcattac cggaaataat ttcgatcggg tagggaatga
     3601 ctttttccgc gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtac
     3661 caactataca gacgctggca ggagaaaacc cttgtagcct ttcggcaaat agcgacagac
     3721 agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt atctggcccc aggccgtccg
     3781 ccagatgcac ccgctgtttc aggcggccaa taatctgcat ggcaccatct accacacggg
     3841 ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag
     3901 gggatttatc gtgtattggc atagggttat tcagattgtt cgagtgattt gatgtagtcg
     3961 taaatcgcca actgcgcccg tactttgcgg cgattgccgc ggggaacgta gcgattactg
     4021 agttctttat cgatataacg tgctttgacc gtatcgctga acaatatgtc gatgatgtcc
     4081 agcacccgct gcttcaggcg cggatcgagc agcggcgttg ccacttcaat acgataatca
     4141 atattgcgcg tcatccagtc ggcggaagaa aggtagacct ttttatcgcc gccattttca
     4201 aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg
     4261 tcgctaatgc cttccagatt ggggatcaga gaacacattc cgcgcaccag cagattaacc
     4321 ggtacgccgg agctggaggc cgcatacaga cgatcaacca ggcctttatc gacaaggtta
     4381 tttagcttca gggtgatacc actgggcagc ccttgctgcg cgttggcgat ctcgcggtcc
     4441 accaattcat acaacagacg gcgagagttt tgcggcgaca ccattaaata atcaaatgtc
     4501 accggacggt atgggttttc aataaagttg aacacccgcc gtacttcgtt agtgatgcgc
     4561 gcatcggcgg tcagcaacga atagtcagta taaagacgcg cggttttttc gttaaagttc
     4621 ccggtcccga tgtgtgcgta acgcaccact tcaccgtttt ctttacgtga aatcaggaac
     4681 agtttggcgt gaattttcag ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc
     4741 aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc
     4801 acggtgactt tcttaccgtt gtgtgcggcg tggatcatcg agtcgatgat gcgtgaatct
     4861 ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa cgaagcctga
     4921 cgcagcagtt ccagtacatg ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt
     4981 tcgcgaatgg catcaaaacc attgcggaac tgggctttat caaaccaaat atggcgtaaa
     5041 cgcggcagtg gtttgttcac cagattggct ttgccgacat tggggaaatt aataaagtct
     5101 ttaaaattat gataacgacc gccggggacg atggagtcgt agcgggaaat agtcagtttt
     5161 tcgcgtaaca cttcaaccag agcattgggc atatcgcgct gataaacaaa acgcaccggc
     5221 tcagcagtta aacgctgctt gagactggaa gacatcaact ccatcagact ggcttccatc
     5281 tcatgcacta aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc attcagtgcg
     5341 tcataatcaa agaagccttt gaaaatatca tcaaggcagt aacgcagaat gttatccaga
     5401 agaatcatcg gcttgcgtcg acgcggcgct tctggcggta aattcacaaa gcgcggcact
     5461 ttatctgatg ggatctccag cagcgcgtaa cggatggtat cgccacggat aatttccacc
     5521 gccagatagg tgtaatcatc tttcaggaac tgcactaagt cagtgtcagg attgattaaa
     5581 atcggcgtaa tgtgctgacg cagatactgc ttaaaataat gacgcagcca gttttgttga
     5641 ttgacggaga gctggcgttc attaatcagg aagatctggt tgcgcgccat ttccagcagt
     5701 agctcgttgt agaggccgtc gaattcctga tcggctttca gcacacggga ctgaattttg
     5761 cccagtaaat ggcgggaatg agagttagag ccttgttctt cgctaataat gatgcgtcgc
     5821 ttcagttcag cgaagcggac tttatagaac tcatcaaggt tattggaata gatccccagg
     5881 aaacgcatcc tttcaatcag cgggttagat ttgtccgccg cttcctgaag cacgcgttca
     5941 ttgaacgata accaactgag ctctttttcg atgtatagct tttcctgacc cattaccact
     6001 tttactccgt tttattcacg ggacactgcc tggatattat ggcgagcaat attggcagat
     6061 gtccaactat gccagaaaag ggggaaaaaa actgctggag cttgcgattt acaggtgaat
     6121 tgaaaaacgc cagcggcaga gctggcgctt taactacggg ggcattactc gtcggcagcg
     6181 tagccctgcg gcggcagacg ttgaccatcc agccacgcgg cgttttcgtg cattttcaga
     6241 cgaccatcgg caaaccagct aatcaccagt ggataaatag cgtgttcctg ggtttgcacg
     6301 cgggcggtga tgtcatcttc cgtatcacca gcaaataccg ggactttcgc ctgtaaaata
     6361 accgggccac cgtccagttc atcggtgacg aaatgcaccg atgtaccgtg ctcctcatcg
     6421 ccattttcca gcgcctgacg atgggtgtgt aatccgggat atttcggcag cagagaaggg
     6481 tgaatgttca gcaaacgccc ggcatagtgg gagacaaacg ccgggctgag aatgcgcata
     6541 aaaccagcca gcacgaccac atcgggtgcg tacatgtcga tttcatgaat caactcccgg
     6601 tcataggctt cacgactgtc aaacgcgctg gtgatgagcg tatgcgttgc aatacccgcc
     6661 tggcgggcgc gttcaaggcc gaacgcgtcg gccttattgc tgaaaactgc ccgtacggtg
     6721 cctttaattt tgttggtttt gcaggcgtca ataattgcct gcaaattact tccgttgccg
     6781 gaaataagca ccacaatatt cattattcga taaccacgcg ttgttcggaa tcagaggctt
     6841 tgatgatacc gattttccac gcgttttcac cgttggcatt gagcagggcg agggctttgt
     6901 ccacttccgg agcaggcagg gcaataatca taccgacgcc gcagttgaag gtgcgataca
     6961 tttcatggcg ctcaacgtta cctgccgttt gcagccagtt aaacacttcc ggccactgcc
     7021 aggaagattc atcaatcact gcctgggtat tatctggcaa tacgcgcgga atgttttccc
     7081 agaaaccgcc gccggtcagg tgcgcaatgg catgcacatc gaccttttca atcaacttca
     7141 gcactgactt cacgtaaatg cgggtcggtg ccagcagatg atcggctaat ggcttaccat
     7201 caagttcggt ggtttgcgga tcacaaccgc tgacttcaag aattttgcgc accagcgaat
     7261 agccattcga gtgcgggccg ctggagccga gtgcaatcag cacatcgccg tcgctgactt
     7321 tagagccgtc aatgatttct gatttttcta ccacgccaac gcagaaacca gcgacatcgt
     7381 aatcttcacc gtgatacatc cccggcattt ccgccgtttc gccacccacc agtgaacagc
     7441 ccgattgcag acaaccttcc gcaatgccgc tgatcaccgc tgaagcggta tcaacatcca
     7501 gttttccggt tgcgtaatag tcgaggaaaa acagcggttc cgcaccttgc accaccaggt
     7561 cattaacgca catggcgacc agatcaatac caatggtgtc gtgacgtttt aagtccatcg
     7621 ccagacgcag cttggtacct acgccgtcag tgccggaaac cagcacaggt tcacgatatt
     7681 tttgcggcaa tgcacacagc gcaccgaagc cgcccagacc gcccatcact tccggacgac
     7741 gcgttttctt cactacgcct ttgattcttc caaccagagc attacccgcg tcaatatcaa
     7801 caccggcatc tttgtagcta agagaggttt tatcggtcac tgcttgggtc cccacgcgtt
     7861 acttgcggta gaaaaataaa attcggcgca attctaacag ggaaagcaaa cgtttgcgag
     7921 actgctttac acaacctttt tgcacgtctt ttccccaggc gcgcggcgaa agaatacttg
     7981 taccagggca aaggatcgtt ttcggatgga ataatcttct tccataacca tttgaatata
     8041 aaataacttt atctcaaacc gttatcattt tgactaaagt caacgaaaat aatattgccg
     8101 ccttgaagaa aggaggtata atccgtcgat ttttttgtgg ctgcccctca aaggagaaag
     8161 agtatgaaga tcgtggaagt caaacaccca ctcgtcaaac acaagctggg actgatgcgt
     8221 gagcaagata tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg
     8281 acttacgaag cgaccgccga cctcgaaacg gaaaaagtca ctatcgaagg ctggaacggc
     8341 ccggtagaaa tcgaccagat caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg
     8401 ggtcttggga tgatggacgg tgtgctggaa aacgttccga gtgcgcgcat cagtgtcgtc
     8461 ggtatgtacc gtaatgaaga aacgctggag ccggtaccgt acttccagaa actggtttct
     8521 aacatcgatg agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt
     8581 atcgcgacca tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg
     8641 gtagctgcac cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat
     8701 actgcatcga ttgaccaggg actgaacgag cacggataca ttattccggg cctcggcgat
     8761 gccggtgaca aaatctttgg tacgaaataa agaataaaaa taattaaagc cgactttaag
     8821 agtcggcttt tttttgagta aagcgcctat aacacataat acagaggata atactatgac
     8881 gcgccgtgct atcggggtga gtgaaagacc gccactttta cagacaatcc cgcttagttt
     8941 gcaacatttg ttcgccatgt ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa
     9001 cccggcgact gtactgttat ttaacggtat aggaacactg ctgtatctct tcatctgtaa
     9061 agggaaaatt ccggcttatc ttggttccag ctttgccttt atttcaccgg tattgttact
     9121 gttgccgttg gggtatgaag ttgcgctggg cggctttatt atgtgcggcg tgctgttctg
     9181 cctggtttct tttatcgtga agaaagcggg gaccggctgg ctggacgtgc tgtttccacc
     9241 tgcggcaatg ggcgcaatcg ttgccgtcat cggtctggag ctggcgggcg tagctgccgg
     9301 tatggcgggt ttactcccgg ctgaagggca aacgccagac tccaaaacca tcatcatctc
     9361 tattaccacc ctggcggtca cggttttagg ttccgtgctg tttcgtggtt tcctggcaat
     9421 tatcccgatt ttaattggcg tgctggtggg gtacgcgctc tctttcgcaa tgggaattgt
     9481 cgataccacg ccgattatta atgctcactg gtttgcgctg ccgaccctct atacgccgcg
     9541 cttcgagtgg tttgccattc tgactattct gccagcggcg ttagtggtta ttgccgaaca
     9601 cgtagggcac ctggtcgtaa cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg
     9661 tctgcaccgt tcgatgtttg ctaacggctt gtcgaccgtg atctccggtt tctttggttc
     9721 cacaccaaac accacttacg gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag
     9781 tacctgggtt atcggcggtg cggcgatttt cgctatcctg ctttcctgcg tcggtaaact
     9841 ggctgctgct atccagatga ttccattgcc ggtgatgggc ggcgtttcgc tgctgcttta
     9901 cggggtcatc ggtgcttccg gtattcgtgt gttgatcgaa tcgaaagtgg attacaacaa
     9961 agcacagaac ctgatcctga cttccgttat tttgatcatc ggcgtcagtg gtgcgaaggt
    10021 aaacattggc gcggcggagc tgaaaggtat ggcgctggcg accatcgtcg gtatcggctt
    10081 aagtttgatc ttcaaactga ttagtatgct gcgccctgaa gaagtggtgc tggacgcaga
    10141 agacgcggat ataacagaca aataatgtac cgaccgggca gtgttgctgc ccggttcaaa
    10201 catcatggga ttctgtggta aacttctcgc gattttgtga aatcctggtt gaggtatctc
    10261 tgaacacacc ggcacagctc tctttgccac tttatcttcc tgacgacgaa acctttgcaa
    10321 gtttctggcc gggggataac tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg
    10381 aacatagcgg ttacatctat ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc
    10441 acgcggcttg cgcggaattg tcacagcgtg gcgatgcggt gggctatgtc ccgctggata
    10501 aacgcacctg gtttgttccg gaagtgctcg acggtatgga gcatttgtcg ctggtctgta
    10561 ttgacaacat tgagtgtatt gccggcgatg agttgtggga gatggcgatt ttcgatctct
    10621 acaatcgaat tctggaatcg ggcaaaacac ggttgttgat caccggcgat cgtccaccgc
    10681 ggcagttgaa tctgggatta ccggatctcg cgtcgcgtct cgactggggg cagatctaca
    10741 aattgcagcc actttctgat gaagataagt tgcaggcgct acagttacgc gcgcgtttgc
    10801 gtggttttga actgccggaa gatgtggggc gtttcttgct gaagcggctc gacagagaaa
    10861 tgcgcacgtt gtttatgacg ctggatcagc tggatcgtgc gtcgattacc gcgcaacgca
    10921 agctgaccat tccttttgtg aaagaaattc tgaagttgta gataaggtgt ttattgtcgg
    10981 atgcggcgcg aacgccttat ccgacctacg cgaagttaat ccatagtatt cgtaggccgg
    11041 ataagacgcg gcaagcgtcg catccggcaa tgtgcttcaa ctagcgatgg agcacatctt
    11101 aacccacgat ttccagtacc tgctccggtg gacgaccaat ccgtgctttg ccattcgcga
    11161 ccacaatcgg gcgttccatt agcttcggat tatccaccat cgcctgaatc agcgcctctt
    11221 cgctaagtga aatgtcagcc aggttcagtt ctttataaag atcctctttt tggcgcatca
    11281 attcacgagc gctattcatc cccagcattt tcagcaaatc acgcagcgtt gccgcatcgg
    11341 cgggtgtctc aaggtagagc accacttctg gctccacgcc gttttctttc agcaggttca
    11401 gcgtttcccg gctctttgaa cagcgtgggt tatggtagat ttttacctgt ttggtcattg
    11461 ttcttccttt aatgcgaatt acatcttggt ataaggctta aagcgttcct gcagctggcg
    11521 caactggtcg atgcgcgcat cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgaga
    11581 actggcgcta ctcaacaagg aaatagcctg atcgagtcgc ccggcgagcg cataaccttc
    11641 tgctcgcgca gccagttcct gatcgcggtt atttagtgcg gcttccgcct gtgccagtaa
    11701 atcccagccg ttgctgtcat ctttattgtt aaaagtgtag cgattcagaa tattggccgc
    11761 ttcctgtggt tgcccccctt gcagataagc gttcgccagg ttgagctgca acaccggatt
    11821 ggtgcgcaaa tcgcgggcat ttttcaggcg attgatagcg tcattggctt tgttttgccc
    11881 aagatcgata tcggtagcca gatcgagata ccatgcgttg ccaggttctg ccgccagtaa
    11941 cggttgcagc gttttacgcg cttcgtcgta tttattggct tccatcgcct gtaaagcacg
    12001 accatattgc gccgctcgtt gctgacgaac gtttcctttc gcccattcat ccagcaaatc
    12061 actggtgagt tggttacgcc ctgaattgta catccccagt gtgcgcactt ttgccagata
    12121 gaaatcttct gacgattgca ccactatcgg gcgcatctga ttggcacggt tacgggcatc
    12181 agccagacgg ctttccggta gtgggtgagt gagcagaatt tctggcgggc gcgaagagta
    12241 acgcgcctga tcgagtaatt tttcgaggaa ggtcggcatc gcctgcggat cgaatcccga
    12301 gcgttgcagc acctgaatac caatgcggtc cgcttcctgt tcattttgct gggtgaaact
    12361 gatcatcccc tggcgcgttc ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg
    12421 actggccatc gccagtaaaa tagaacctaa cgcgccgacc caggtcagcg gcgcgctgcg
    12481 ctgctgatct tccatcgctc gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc
    12541 cataactgaa gccagttgac tttcgttatc ggaataacgg aacagggcag agtgcagcac
    12601 cacgttgccg ccaaagaaag caaaggcgtt aatttcatcg ttgttgatca gaaagaaatg
    12661 aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc attcctagcg aattaatgta
    12721 ttgcgttaac agcggatcat taattaacgg cgcgctgccg cgtaactggc ggacataata
    12781 gtcgcccatc tgcatttcct gaccaatgga aagtgtgctt cctgcggagg ttcccatatc
    12841 cggcaaggtg tctgcgctgt cggcaaacgc cggggctacc tgaccaatgg tcatagcagc
    12901 aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac ataacctcta tcctgtattt
    12961 ctggagatag tcatttgacc gatactgtga aatatcgttc ccaccgcgcc gcctttgagt
    13021 gtaacggcga agattagccc actcaaatga cttttcagtt tcctgaatcc gtcataaaaa
    13081 gcgaagtctt ttttgtgaca tttcgataca attgcggatc gcaatcccgc tcttgaggtt
    13141 cagggaaggt ctgtatgctc gaaatgttga tgcaatggta tcgccgccgt tttagcgacc
    13201 cggaagcgat tgccttgctg gttattttgg ttgctggttt tggcattatc tttttcttta
    13261 gcggcctact cgcgccgttg ctggtggcta ttgtgctggc ctatttactg gaatggccga
    13321 ctgtccgcct gcagtctatt ggctgttccc gcccttgggc gacatctatc gtgctgatcc
    13381 tgtttgtggg catcctgcta ctgatggcgt tcgtggtact gcctatcgcc tggcagcagg
    13441 gcatctactt aatccgcgat atgccgggga tgctcaataa gctttctgac tttgccgcca
    13501 cgttgccgcg ccgctatccg gcgttaatgg atgcgggcat tattgatgca atggctgaaa
    13561 atatgcgcag taggatgctg accatgggcg attcggtggt gaaaatttcc ctcgcctcgt
    13621 tggttggttt gctgaccatt gccgtctatc tggtgctggt gccattgatg gtcttcttcc
    13681 tgctgaaaga caaagagcag atgctgaacg ccgttcgccg ggtgctgccg cgcaaccgtg
    13741 gactggcagg acaggtgtgg aaggagatga atcaacaaat caccaactat atccgcggca
    13801 aagtgctgga gatgatcgtg gtagggatcg ccacctggct ggggttcttg ctctttgggc
    13861 tgaactattc gctgctgctg gcggtgctgg tcggcttctc ggtacttatt ccgtacattg
    13921 gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct attccagttt ggtgctggca
    13981 cggaattctg gagctgcttc gcggtgtatc tgattattca ggcgctggac ggcaacctgt
    14041 tagtaccggt gttgttctcc gaagcggtta acctgcatcc tctggtgatt attttatcag
    14101 tggtgatctt cggtggtttg tggggattct ggggcgtatt cttcgctatt ccattggcga
    14161 cgctgatcaa agccgtgatt cacgcctggc ctgatgggca aatcgcgcaa gactgacctt
    14221 cagcgaaaaa attttaaacg agtgttgcag ggcagtgaga agatttgaga tgtgctggcc
    14281 gcaagcgcag ccagcacgga atggagcaaa gtaatcaggc gtgttctttc agccagttca
    14341 gcacaacgtc gtggtgattg ctggttttga aatcgtcaaa gacatgttcg attttgccat
    14401 cagcgtcaat caggaagctg atgcgatgaa taccatcgta ggttttgccc atgaaggact
    14461 tttcacccca gacgccgaat tgttcgcaca cctggtggtc ctcatcagac aggagcgtaa
    14521 agttaagcag ctctttttcc gcaaaacggg agagtttttc gggtttatcg gtgctgatac
    14581 ccagcacatc aacgcccgct tttttcaact catccatgtt atcgcgtaag ccgcaggcct
    14641 gtacggtaca gccgggggtc atggctttcg ggtagaaata aaccagaaca cgctgtccct
    14701 ggaagtcggt caaattaact tgttctccgt cttgatccgg caagctaaat ttcggtgcga
    14761 tatcaccggc tttcagtgga ttcattactt aactccatcc tgttcatcat gttgggaata
    14821 attgacgacg ttaatactgc cttgtgcatt gagttctgta catagggctt tgaacgcttg
    14881 ctcaatattt gctgcgtccg cagatgcggg gctgtgggca gttatctgaa tatgcaactg
    14941 cgcagcccgt tcattttcag caggttgcgt gcgcgacacc agctccgcaa tgttcatatg
    15001 atgcgcgtcg aaaagtgctg tgaagcgttc aattaaatgc ggggagtctg ccacatcgac
    15061 ctgaacccag acagatgctg gcattggcgg acgcggacgc gccgtcgtgc gcttcatcac
    15121 gattaaaaga tccagctcgg cacctttcaa cggtaacgtt gattcaatca gagtaatggc
    15181 attccatgaa ccggaaagca gcatgataaa cgtgaactct tctcccagca tcgccaggcg
    15241 actgtcttca atattacagc cgcaactact gacatgacgg gtgatggtgt tcactattcc
    15301 agggcgatcg gcacccaacg cagtgatcac cagataatgt tgcgatgaca gtgtcaaact
    15361 ggttattcct tcaaggggtg agttgttctt aaggaaagca taaaaaaaac atgcatacaa
    15421 caatcagaac ggttctgtct gcttgctttt aatgccatac caaacgtacc attgagacac
    15481 ttgtttgcac agaggatggc ccatgttcac gggaagtatt gtcgcgattg ttactccgat
    15541 ggatgaaaaa ggtaatgtct gtcgggctag cttgaaaaaa ctgattgatt atcatgtcgc
    15601 cagcggtact tcggcgatcg tttctgttgg caccactggc gagtccgcta ccttaaatca
    15661 tgacgaacat gctgatgtgg tgatgatgac gctggagctg gctgacgggc gcattccggt
    15721 gattgccggg accggtgcta acgctactgc ggaagccatt agcctgacgc agcgcttcaa
    15781 tgacagcggt atcgtcggct gcctgacggt aaccccttac tacaatcgtc cgtcgcaaga
    15841 aggtttgtat cagcatttca aagccatcgc tgaacatact gacctgccgc aaattctgta
    15901 taatgtgccg tcccgtactg gctgcgatct gctcccggaa acggtgggcc gtctggcgaa
    15961 agtaaaaaat attatcggaa tcaaagaggc aacagggaac ttaacgcgtg taaaccagat
    16021 caaagagctg gtttcagatg attttgttct gctgagcggc gatgatgcga gcgcgctgga
    16081 cttcatgcaa ttaggcggtc atggggttat ttccgttacg gctaacgtcg cagcgcgtga
    16141 tatggcccag atgtgcaaac tggcagcaga agggcatttt gccgaggcac gcgttattaa
    16201 tcagcgtctg atgccattac acaacaaact atttgtcgaa cccaatccaa tcccggtgaa
    16261 atgggcatgt aaggaactgg gtcttgtggc gaccgatacg ctgcgcctgc caatgacacc
    16321 tatcaccgac agtggtcgtg agacggtcag agcggcgctt aagcatgccg gtttgctgta
    16381 aagtttaggg agatttgatg gcttactctg ttcaaaagtc gcgcctggca aaggttgcgg
    16441 gtgtttcgct tgttttatta ctcgctgcct gtagttctga ctcacgctat aagcgtcagg
    16501 tcagtggtga tgaagcctac ctggaagcgg caccgcttgc ggagcttcat gccccggctg
    16561 gaatgatctt gccggtgacc tccggtgatt atgcaatccc ggtgaccaac ggtagtggtg
    16621 ctgtcggtaa ggcgctggac attcgtccac cagcccagcc gctggcactg gtttctggcg
    16681 cgcgtaccca gttcacgggc gataccgctt cattgctggt ggaaaatggt cgtggcaata
    16741 ctctgtggct gcaggtggtt agcgtgctgc aggcgaaaaa ctacaccatc acccaacgtg
    16801 atgatgctgg tcagacactg accaccgatt gggtacaatg gaaccgtctg gacgaagacg
    16861 agcagtatcg tggtcgttat caaatctctg ttaagccgca gggttatcag caggcggtta
    16921 cggttaaact gctgaacctg gaacaggcgg gcaaaccggt tgcagacgcg gcttccatgc
    16981 agcgttacag cacggagatg atgaacgtta tttccgccgg tctggataaa tctgccactg
    17041 acgccgcgaa cgctgcgcaa aatcgtgcct ccaccactat ggacgtacaa agtgcagctg
    17101 atgacaccgg tttaccaatg ctggtcgtac gcgggccgtt caatgtggtt tggcaacgtc
    17161 tgccagctgc gctagaaaaa gtgggcatga aagtgaccga cagcacccgt tcgcagggca
    17221 acatggccgt aacttataag ccgctgtctg acagcgaatg gcacgaactg ggcgcgagcg
    17281 atccaggtct ggcatccggt gactataaac tgcaggttgg cgatttagat aaccgcagca
    17341 gcctacagtt catcgatccg aaaggtcata ctctgactca gagtcagaac gacgcgctgg
    17401 tagctgtctt ccaggctgcg tttagcaagt aaaaatacag ggctggagtc atccggccct
    17461 tttttctgat atgatacgca aacgtgtgcg tcgacagaaa aacgcgattt tagcggtaat
    17521 tcgcacgaaa tttgtttgtc ggacgtaggt cggataaggc gtttacgccg catccgacaa
    17581 aacatccggc acaccagaca gcaaaagatt ttaaaacgtt aattcacacc caggagtgat
    17641 aaagatgcaa aagcaagctg agttgtatcg tggtaaagcg aaaaccgtat acagcacgga
    17701 aaacccggac ctgttggtgc tcgaattccg caatgatacg tcagcagggg atggcgcgcg
    17761 cattgagcag tttgatcgca aaggtatggt gaacaacaag ttcaactact tcattatgag
    17821 caaactggct gaagcgggta tcccgactca aatggagcgt ctgctctccg ataccgaatg
    17881 tctggtgaaa aagctggata tggtgccggt tgagtgtgtc gtgcgtaacc gtgctgctgg
    17941 ctctctggtg aaacgtcttg gaatcgaaga aggtattgag ctgaacccgc cactgttcga
    18001 tctgttcctg aaaaacgacg ccatgcacga tccgatggtc aacgaatctt actgcgaaac
    18061 ctttggctgg gtgagcaaag agaacctggc gcgtatgaaa gagctgacct acaaagcgaa
    18121 cgacgtgctg aaaaaactgt tcgatgatgc tggtctcatt ctggtcgact tcaagctgga
    18181 gtttggcctg tacaaaggtg aagtggtact gggcgatgag ttctccccgg acggcagccg
    18241 cctgtgggac aaagaaacgc tggagaaaat ggataaagac cgtttccgcc agagcctcgg
    18301 tggcctgatc gaagcctatg aagccgttgc ccgccgcctg ggtgtacagc tggactgatt
    18361 tttctgttca tcatcttgcc gtgctgctgg cacggcaaga caaccgctct cataagatgt
    18421 gcattgaacg ctcattcctc cgccatttca tcccgctttc ctgtggtaat cttcctctga
    18481 accacctacg atcatttcta taatgaatat aatgttgagg tatctatgcg ttggcaaggg
    18541 cgacgtgaaa gtgacaatgt tgaagacagg cgcaatagct ccggcggtcc gtcaatgggc
    18601 ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga ttttactgat agtcgtactg
    18661 gttgcaggct actatggcgt tgatttaacc gggttgatga ccggacagcc ggtttcccaa
    18721 cagcaatcaa cgcggtcaat tagccctaat gaagacgaag ccgcaaaatt cacctcggtg
    18781 attctggcaa ccacggaaga cacctgggga cagcaattcg agaagatggg taaaacctat
    18841 cagcaaccga agctggtcat gtaccgtgga atgacgcgta ccggctgcgg ggcgggccag
    18901 tccataatgg ggccattcta ttgcccggcg gatggcacag tttatatcga tctctccttc
    18961 tatgatgaca tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg gtacgttatc
    19021 gctcatgaag tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa agttcgtcaa
    19081 ctgcaacaaa acgcgacgca ggcggaagta aaccgcttat ctgtgcgtat ggaactccag
    19141 gccgactgtt ttgccggtgt ctgggggcat agtatgcagc agcaaggtgt tctggaaacc
    19201 ggcgatctgg aagaggcgct gaacgcggcg caggccatcg gcgatgaccg tttacaacag
    19261 caaagtcagg ggcgagtagt accagacagt ttcactcatg gcacttctca gcaacgctac
    19321 agctggttta aacgtggttt cgacagcggc gatccggcac aatgcaatac ttttggtaaa
    19381 agcatttaac ttccggggca gggatggctg aactgactgc gcttcacaca ttaacagcgc
    19441 aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag cggggaagag cgttggtgtt
    19501 ttgatcatgc gcttaagttg cgtgatgcct tacctggcga ctggctgtgg atttcgccgc
    19561 agccagatgc tgaaaaccac tgttctccct cggcactaca aactttactt gggcgcgagt
    19621 tccggcatgc ggtattcgac gcccgccaag gctttgatgc cgctgccttt gcggcactta
    19681 gcggaacgtt gaaagcggga agctggctgg tgttgttact ccctgtatgg gatgagtggg
    19741 aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc
    19801 cgcattttgt ccagcatttc aaacgcgtac ttacggcgaa taacgacgct atcctctggc
    19861 ggcaaaacca gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcta
    19921 ccggcgcacc acaaccagaa caacagcaac tcttacagca gctactgacc atgccgccgg
    19981 gcgtggcagc ggtaactgct gcgcgtgggc gcggtaagtc ggccttggca gggcaactca
    20041 tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca acggatgtac
    20101 tggcacaatt tgcgggcgag aagtttcgct ttattgcacc ggatgccttg ttagccagcg
    20161 atgagcaagc cgattggctg gtggtcgatg aagccgcagc tatacctgcg ccgttgttgc
    20221 atcaactggt atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag ggctacgaag
    20281 gcaccggacg tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg
    20341 aactacaaca gccgatccgt tgggcgcagg gatgcccgct ggaaaaaatg gttagtgagg
    20401 cactggtttt tgacgatgaa aacttcaccc atacaccaca aggcaatatc gttatttccg
    20461 catttgaaca gacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat
    20521 tgtctggtgc gcactaccgg acttcgccgc tggatttacg ccggatgatg gatgcaccag
    20581 ggcaacattt tttacaggcg gctggcgaaa acgagattgc cggggcgctg tggctggtgg
    20641 atgagggcgg attatctcaa gaactcagtc aggcggtatg ggcaggtctt cgtcgcccgc
    20701 ggggtaatct ggtggcccag tcgctggcgg cgcacggcag caatccactg gcggcgacat
    20761 tgcgcggaag gcgggtcagc cgaatagcag ttcatccggc tcgtcagcgg gaaggcacag
    20821 gtcagcaact gattgctggt gctttgcaat atacgcagga cctcgactat ctttcggtga
    20881 gttttggtta caccggggaa ttatggcgtt tctggcaacg ctgcggtttt gtgctggtgc
    20941 gaatgggtaa tcatcgtgaa gccagcagcg gttgctatac ggcgatggca ctgttaccga
    21001 tgagtgatgc gggtaaacag ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc
    21061 aagctctcgc gcagtggaat ggcgaaatgc ttcctgttga tccactaaac gatgccgtcc
    21121 tttctgacga cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa
    21181 cgtcgttagg ttgcttaatg cgtctgttac aaaccagcga actggcatta ccggcgctgc
    21241 gtgggcgttt acagaaaaac gccagtgacg cgcagttatg taccacactt aaactttcag
    21301 gccgtaagct gttactggtt cgccagcggg aagaggccgc gcaggcgcta ttcgcacttg
    21361 atgatgttcg cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac
    21421 tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt
    21481 aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa accagcacaa
    21541 cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggc
    21601 agctggtttg cgccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg cgcggagccg
    21661 agcggtaatc cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc
    21721 caggcgcgtg tggatgccat catgccgacg tttattgaga cggtgcgcta ctggcagaaa
    21781 caaagcggag taggggctaa tgccacggcg ctaatcggtt tctcacaagg cgcgattatg
    21841 gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc gcgtcattgc cttcaacgga
    21901 cgctacgcga gcctgccgga aaccgcgtct acagccacaa caattcactt gattcatggc
    21961 ggtgaagatc cggttatcga tctggcgcat gcggttgcgg cgcaggaagc gttaatcagt
    22021 gccggtggcg atgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc
    22081 agtatgcaat tagccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa
    22141 gcgctaagcg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta ataatagccg
    22201 ggtaggtctg ataagactgt atcaggcatc atggccggat gcggcgttaa cgccttatcc
    22261 ggccacttag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    22321 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttatgg
    22381 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    22441 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    22501 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    22561 gcgttcacac attcattaat tttatgaatc gtggcattga ccggcccgag ttccaccacc
    22621 tgcgccccca tgcgggcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22681 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22741 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22801 tcaagcaggg caagcacctg cgctttgatc atctcatccg tcagttcggt gctaaagcgg
    22861 aagttaaact gcacaaacag ttcacctgga ataacgttgt tactgcccgt tcccgcctga
    22921 atattggcaa tctgcatact ggtcgccggg aagaattcat taccctgatc ccactcaata
    22981 gccactaatt cattaatgaa aggtgctgcg cgatgtaccg gattgtcagc cagatgtggg
    23041 taggcaacat gcccctgaac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    23101 ccatttttca ccacatcacc taccacttcg atactcgacg gttcgccaac cagacagtaa
    23161 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc attgtgggca
    23221 ctagcttctt catcagaggt gatcagaaat gccagtcgcc ccgtatggtt gggatgttgt
    23281 gcgacaaagc gttctgccgc tactaccatc gccgccagcg agcctttcat atctgccgca
    23341 ccgcgcccga ataacatgcc gtcacgaatg gttggttcaa acggcggatt gatccaacga
    23401 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    23461 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    23521 atcgcctgca aacgttcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    23581 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23641 tggaaaaatt gctgataact ggattcactg aaacccagca gcataggctt accaggcgcg
    23701 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgtcgc tgcagaggcc
    23761 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23821 ttgagtaacg cttcccagcc taattcgttg ataaaaccgt tcaataattc gctgtccaat
    23881 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tagcttccag ccaacggcga
    23941 gccttcttaa tggtgtcaca atttttgatg ccgtaaagtg taaccataat gaatcctttt
    24001 tcgatttatg agaataaata ctcatttaaa ggtgaaaaca ttttgcctgc aaatgattat
    24061 caggaaatat aacattatcg aagtattcaa attaacaacc attaaataac attaaacatt
    24121 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa ccagaattaa
    24181 atcagtatta gatgccgtat gaatatctcg aaatgttgcg gtgcatcaca tacaaatcat
    24241 caccgcagga ccatagtatt tacataaggt ttgtcccccg gaggtagtca tcacagcaat
    24301 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    24361 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    24421 taagtaattc ctgagacaaa gtacaaacag caagaacccg ctgagcaggt tcttaatcgg
    24481 aaagttgaaa agggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    24541 cttcagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24601 aatagtggcc gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24661 accggcacct gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24721 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24781 tttttggttc atctcgttag caaactcgac gatcaggata gcgtttttcg ccgataaacc
    24841 aatgaccgtt aacagaccca cctggaagta aacatcgttt tccagcccgc gcatccaggt
    24901 tgccagcagc gcgccgataa cccccagcgg cacgaccagc attaccgaga acggcaccga
    24961 ccagctttca tacaatgccg ccagacacag gaataccacc agcaaggaaa tggcgtacag
    25021 cgccggagcc tgcgcgccgg aaagccgctc ctgatacgac atcgccgtcc actccagacc
    25081 aaagccgttt ggcaactgct tcactaacga ttccataata tccatcgccg taccggtact
    25141 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccattat agcgttccag
    25201 acgcggcgag cctgtctccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    25261 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    25321 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctaga
    25381 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    25441 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    25501 acgggttagc tccgggtttt ccgctgccag cgccagcaac tggtttcgtg ctgccatcag
    25561 cgcatcgtga cccgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25621 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25681 cgccttcgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25741 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25801 acccacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25861 gatttgctca acgactttca gggtctgttg ctgggttgaa ccgctgggca actgtaccga
    25921 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25981 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tacggtggag
    26041 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    26101 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagcgtggc
    26161 acacagagca ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    26221 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggcac
    26281 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    26341 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    26401 caccaccacg atggcatcat ccaccagcag accgatcgcc agcaccatcg cgaacatggt
    26461 taaggtgttg acactgtaac cgaaggcgta aagcacggag aaggttccca tcaacaccac
    26521 tggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    26581 gaggaaaacc agggcgatag cttccagaag cgttttcacc acgtcttcaa tggaggcttt
    26641 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgtggaa aatactgcgc
    26701 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgttcg cgccggaggc
    26761 cagtttaacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26821 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcctgatt
    26881 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26941 ggtggcgttg agcgcctgct tatcgacgga aggcgtacca ccaagttgcc caaccgcaat
    27001 ctgcgcgttc tgtgattcaa tggcatcggt gacatcttta gccgtcatct ggaaactgtt
    27061 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    27121 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    27181 atcctgttta tccatcgaac catcggtaga gacgaaggca atggtcagaa tgttggtatc
    27241 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    27301 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    27361 acttaacgtg acagatgcct gaccggtgcc gctgctctga gatgacatat acatcagatt
    27421 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    27481 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    27541 ttcaacgggc aatgaaaaaa tcgccagggt acctgtcaga cacaacagga ttgccagcac
    27601 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27661 tgcgtatcgt tgtaggcttg gcttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27721 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgccact
    27781 attctttggt caataagagg cattacatca gctgactttc ctcaccctcc gcagagcgaa
    27841 agctaatact cactaacgtg ccgccgccgg aaggttgtga aaaagtcagc gtcccaccca
    27901 atctttccgc gcgttcgcgc atgatattca gaccataatg gccttcgggt tctttcggtt
    27961 caccgatgcc aatcccgttg tcgcgaatat agactgtgtg attgccatcc ggcgcggtga
    28021 cgcagctaac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    28081 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    28141 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    28201 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    28261 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    28321 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    28381 gagccagtga gtcgtgcaat tcgcgggcga tggtcgcacg ttcttccatc aacaataact
    28441 gctgaaaatg cttctgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    28501 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    28561 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggctgccctt
    28621 cgctaatccg ccagttgtca ccgacattta actccagata ttcagccgct tcattgtcgc
    28681 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggctggtg tttagcgcct
    28741 gcgaacactg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28801 cttctactga cgcctccagc gaacggtaca atttatgcag ctcgctcgac atctggttaa
    28861 aggtttttgc aagcaggcca agctcattcg gcaggctggt atccagcggc ggcgagtcga
    28921 actgcccgtg ttcaatacgc tgactggcgg taaccagctg attcagcggg gcaaccacct
    28981 gatggcgtat gcgacgcaaa gtaaaaaaga ccagcgtgaa aataccgatg ccgccagcca
    29041 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagtacga
    29101 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcaccct
    29161 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgggttttta
    29221 ctgcctctgg cacataccag acgttgagat tggttaatac cggtgaatgc agtgcctgct
    29281 gaaataactg gcgatgtgca ttgagttgtg gactgccact ttgcaaatcg tagcccaggc
    29341 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    29401 tcgccagcgt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    29461 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    29521 aaaatcatta atgttccagt tcgctgaaac gcaatttagt cagtgatggg attatttgat
    29581 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29641 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29701 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29761 gtgaaatcgc ctgtgtcatg gctcacaatg acgagcaaca tgtcctgagc caacaccatt
    29821 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29881 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29941 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    30001 catttggtac gatgcaaatc gttctgacac ccgtcgcggc aggaaaagta aaagccacgg
    30061 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gaaaactgcc
    30121 cggcggacgc gctgcaactg gtcactgacg ccgcactctc cggcatggcg aaatcccgcc
    30181 gcttgcgcac cgcacgtcag gaacaacaac cgtggcatgc cagtaccgcg gcgcaagaaa
    30241 tgccggtaat gagtaaagtc gagcaaatgc aggcaacgcc cgcgcgtggc gagccagata
    30301 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    30361 atcaggcaca acgggaagcc tcgcgctgcc ttaagtgcgg cgagcacagc gtttgtgaat
    30421 ggacctgccc gctgcataac catataccgc agtggattga actggtgaaa gccggaaaca
    30481 tcgacgccgc cgtcgagctt tctcaccaga ccaacaccct gccggaaatt accggacgcg
    30541 tttgcccgca agaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcag
    30601 taactatcgg taacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30661 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30721 cagggctggc ctgtgcagat gttctcaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30781 gccatccaga aatcggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30841 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30901 gtgaagtggg taaagatgtc tctttgaatt cgcttttgga acaatacgac gcggtcttcg
    30961 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    31021 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    31081 taccggaaga gccgtttatc aatacggccg gacttaacgt cgtggtactg ggcggcggcg
    31141 acaccgcgat ggactgtgtg cgtactgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    31201 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgtg
    31261 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgaactg aatgaacaag
    31321 gtcacgtctg cgggattcgt ttcctgcgca cacgtcttgg cgagccggac gcccaggggc
    31381 gtcggcgtcc agtgccggtg gaaggcagtg aatttgtcat gccagccgat gcggtgatta
    31441 tggcgtttgg cttcaatccg cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    31501 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accacgaatc
    31561 cgaaaatctt cgccggtggt gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31621 cagaaggacg tcatgcggca caggggatca ttgactggct gggcgtaaaa tcagtcaaat
    31681 ctcactgata gcctgcgcag acaaacccga cttcacagcg taagataatt gttcatttcg
    31741 cgctgtggag tcggtatgac gcaacaaatc accctcgtta aagacaaaat tctctccgat
    31801 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31861 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31921 aagaaaaaga ccgtggttct gattcgccag ttccgcgtcg ctacctgggt taatggcaat
    31981 gaaagcgggc aactgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    32041 tgcattcgca aagaagcgat tgaagagacg ggctatgaag ttggcgaagt ccgcaaatta
    32101 tttgaactgt atatgtcgcc aggcggtgtg actgagctaa tccacttttt tatcgccgaa
    32161 tacagtgaca gccagcgcgc taacgccggt ggtggtgtcg aagatgaaga tattgaagtg
    32221 cttgagctgc cgttcagcca ggcgctggag atgattaaaa ccggcgagat acgtgacggt
    32281 aagacggtgt tattgcttaa ctatttgcaa atgtcacatt taatggactg aaaaataaca
    32341 ataatatttc gttgtttatt attggctcaa tccgacaaat ccgattgagc cgcgctacct
    32401 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    32461 gatatcgcat tttccttctc ttttttttcg ctttgttgcc gatgtctttg gtgtgggcgg
    32521 caccagcgca acgggcgttt tccgactggc aggtcacctg taataatcaa aatttctgtg
    32581 tagcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32641 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtcag
    32701 aaggggagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32761 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32821 tgcagatgat tcaggaaggg agggcgatca ccttacgcga cggcaatcag actatttctc
    32881 tcagtggctt aaaagcggcg ttgttgttta ttgatgccca gcaaaagcgc gttggcagtg
    32941 agaccgcgtg gataaagaaa ggggacgaac cgccgctcag cgtaccgcca gcgcctgcgc
    33001 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgtaacg
    33061 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    33121 gtcgtgaggt gaatgtcact gcgctgaccg atgataaagc gctgatgatg attagctgtg
    33181 aggctggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctgg
    33241 cctcgcgccc ggtacggttg cgtttgccgt tcaacaacgg tcaggagacc aatgaactgg
    33301 aactgatgaa cgcaacattc gatgaaaaat cgcgtgaact ggtgacctta gcaaaagggc
    33361 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    33421 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    33481 cgttgtggat cacccgttaa acaaaatggc ggggacatgt agaccggata aggcgcacca
    33541 gcgtcacatc cgacaatcag catccggcga tcaccatcag gcacctttca ctcccagtac
    33601 cttatgcgct tttgccacaa tattctcggc ggtaaagcca aagaacggga acagcttatc
    33661 cgccggagct gattccccat aacctgtcat cccgacaatt gcccctttca ggccaacata
    33721 tttgtaccag taatcggcaa tacccgcttc tactgccacg cgggcagaaa catttgaagg
    33781 cagtaccgac tcccgatatt tctcatcctg ggcatcgaaa atatcggtcg agggcaggga
    33841 aactacgcga acattgcgac cttctcccgc taatttctct gccgcttgca gggtgatttc
    33901 catctctgaa ccggtggcaa tcagaataat atctggctta ccgccgctgt ctttcagcac
    33961 ataaccgcca cgagcaatct ctttaacctg atccggtgtt cgttctacct gtgccagatt
    34021 ctgcctggag aggatcagtg ctgtcggtcc gttgtggcgc tcaaccgcca gcttccagcc
    34081 aaccgccgct tccacctgat cgcacggtcg ccaggtgctg aaatttggcg ttaagcgcag
    34141 actagccagt tgctcaacag cctggtgcgt cggaccatct tcgcccagac caattgagtc
    34201 gtgggtataa accataatct gccgcgcttt catcagtgcc gccatccgcg cggcgttacg
    34261 ggcgtattca acaaacatca ggaacgtcgc ggtatacggc acaaagccgc cgtggtgcgc
    34321 gatgccgttg gcgatagcgg tcataccaaa ttcacgcacc ccgtagtgaa tgtagttgcc
    34381 cgccggatct tccttcagcg aaacagaacc tttccagatg gtcaggttgc tgggagccag
    34441 atccgccgaa ccgccgagca actcaggcag catcggcccg taagcgttaa gcgtattttg
    34501 cgaagcctta cgggtagcga ttttcgccgg attcgcctgc aactcattga tatatttctg
    34561 agtcgttttc tcccagtcct tcggcaaacc gccgctcatc cgacgggtaa actcttctgc
    34621 cagttgcgga tgagcctttt tataggcggc aaacttctca ttccagctct gctgcgcttt
    34681 ttcgcctttt tcgcgggcat cccaggcgtg ataaatctct ttagggatct caaatggcgg
    34741 atggtgccag cccagttttt gccgtgccag cgccacttct tcttccccca gtggtgcgcc
    34801 gtgcgcctct tccttacctg ctttattcgg cgaaccaaag ccaatcaccg tacggcagat
    34861 aatcagcgac ggcttatctt tcacgctttg cgcttcaagg atcgcttcct tcaccgcctg
    34921 tggatcgtga ccgtcgattt catggatcac atgccagtga taggcttcaa atcgttttgc
    34981 ggtatcgtcg gtaaaccagc cttcggtttc accgtcgatg gaaataccgt tgtgatcgta
    35041 aaaaccaatc agcttgccca gccccagcgt gcccgccagc gaacagactt cgtgagagat
    35101 accttccatc aggcagccgt cgcccataaa cacataggtg aagtgatcga caatttcatg
    35161 atccggctga ttaaactgcg ccgccagcgt acgctctgct atcgccagcc cgacggcgtt
    35221 cgccaaacct tgtccaagag gaccggtggt ggtttcaaca cctggcgtat agccgatttc
    35281 cgggtgacca ggggttttcg aatgcaactg acggaagttc ttcagttctt ccagtggcag
    35341 gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc gacgcgtgac cgttggaaag
    35401 aataaagcgg tcacggtcat accaggttgg gtcggtgggg ttatgtttaa gaaaatcgtt
    35461 ccacagcact tcggcaatat cagccatgcc catcggcgcg ccgggatgac cagaattggc
    35521 tttttgtacc gcatccatac tgagtgcgcg aatcgcattg gcaaggtctt ttcgggacat
    35581 ataacactcc gtggctggtt tatagtttgg cggcaagaag atcttccagt ttgcgttgat
    35641 caacggcgaa cagacgaatg ccttcagaca gtttttctac cgccatcgca tcctgattgt
    35701 gctcccaacg gaactccgct tcgctcatgg gagctgggcg tgggaacgtc tgagaaggtg
    35761 ggattaattt acgtaccact ggcgaaactt tttcctgtag ctccttcagc aaattcggtg
    35821 cgatagtcag tcggtcgcag ccggttaagg cgaggatttg ttcggtgcga cggaagctcg
    35881 cgcccatcac aatggtttca tagtggtgtt gcttatagta gtcgtagata ttgcgtactg
    35941 atttaacgcc tggatcttct tccaccacat acgggtccat cggcttgcgt gcctgatacc
    36001 agtcataaat acgcccgaca aacggcgaaa tcagaaaaac gcctgcttct gcacaggccc
    36061 gcgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt aataccttct ttttccagct
    36121 cttctgccgc gcgaattcct tcccaggtcg aagccagctt gatcagaatg cgtgatttct
    36181 caacgccttg ttgctgatac aagtccacca gatggcgcgc cttctcaata ctcttttctt
    36241 tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg accgggtacg attttgagaa
    36301 tttcagcacc gaaattgacc gccagtttgt cacacgctgc gaccacctgt tgttcctggg
    36361 tcttgccatt ttttttaccc caggcgatag catcgtctat taaatgctca tattgtgata
    36421 atccggcagc cttgagtaac agcgaaggat tggtggtggc atcctgggga tgataatggc
    36481 gaatggactc aatatcgccg ctgtctgcca cgacagtggt gaactgtttg atgccgtcta
    36541 actcgttcat aggaaatact ccttgaaaag taaagtgtta gatgagtgcg ttaattcaca
    36601 cttctgagaa atttcgctaa acgcatcaaa aaagcatagc agacaggcat ggtattgctg
    36661 gattaagcag gtaacatcag tgttatagga ttattaccaa aacattatat taatggtgag
    36721 agtttggact tgctcaaagt ctgtagactc cggcagggta ataatgtgcg ccacgttgtg
    36781 gacaggggct gttgcccaca cactttattt gtgaacgtta cgtgaaagga acaaccaaat
    36841 ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa tttccagttc cagggaaaat
    36901 ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat ctggcgctgg cctactcacc
    36961 aggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg ttaaaagcct acaaatatac
    37021 cgcgcgcggt aacctggtag cggtgatctc taacggtacg gcggtgctgg ggttaggcaa
    37081 cattggcgcg ctggcaggca aaccggtgat ggaaggcaag ggcgttctgt ttaagaaatt
    37141 cgccgggatt gatgtatttg acattgaagt tgatgaactc gacccggaca aatttattga
    37201 agtcgtcgct gcgctcgaac cgaccttcgg cggcatcaac ctcgaagaca ttaaagcgcc
    37261 agaatgtttc tatattgaac agaaactgcg cgagcggatg aatattccgg tattccacga
    37321 cgatcagcac ggcacggcaa ttatcagcac tgccgccatc ctcaacggct tgcgcgtggt
    37381 ggagaaaaac atctccgacg tgcggatggt ggtttctggc gcgggtgccg cagcaatcgc
    37441 ctgtatgaac ctgctggtag cgctgggtct gcaaaaacat aacatcgtgg tttgcgattc
    37501 aaaaggcgtt atctatcagg gccgtgagcc aaacatggcg gaaaccaaag ccgcatacgc
    37561 ggtggtggat gacggtaaac gtaccctcga tgatgtgatt gaaggcgcgg atattttcct
    37621 cggctgttcc ggcccgaaag tgctgaccca ggaaatggtg aagaaaatgg cccgtgcgcc
    37681 aatgatcctg gcgctggcga acccggaacc ggaaattctg ccgccgctgg cgaaagaagt
    37741 gcgtccggat gccatcatct gtaccggccg ttccgactac ccgaaccagg tgaacaacgt
    37801 cctttgcttc ccgtttatct tccgtggcgc gctggacgtt ggcgcaaccg ccatcaacga
    37861 agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg gcccatgcgg aacagagtga
    37921 agtggtggct tcagcgtatg gcgatcagga tctgagcttt ggtccggaat acatcattcc
    37981 aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct gcggtggcga aagcggcgat
    38041 ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac gtctacatcg acaagctgac
    38101 agagttcgtt tacaaaacta acctgtttat gaagccgatt ttctcccagg ctcgcaaagc
    38161 gccgaagcgc gtagttctgc cggaagggga agaggcgcgc gttctgcatg ccactcagga
    38221 actggtaacg ctgggactgg cgaaaccgat ccttattggt cgtccgaacg tgatcgaaat
    38281 gcgcattcag aaactgggct tgcagatcaa ggcgggcgtt gattttgaga tcgtcaataa
    38341 cgaatccgat ccgcgcttta aagagtactg gaccgaatac ttccagatca tgaagcgtcg
    38401 cggtgtcact caggaacagg cgcagcgtgc gctgatcagt aacccgacag tgatcggcgc
    38461 gatcatggtt cagcgtggcg aagccgatgc aatgatttgc ggtacggtgg gcgattatca
    38521 tgaacacttt agcgtggtga aaaatgtctt tggttatcgc gatggcgttc acaccgcagg
    38581 tgcaatgaac gcgctgctgt tgccgagtgg taataccttt attgccgata cctacgtcaa
    38641 tgatgagcct gatgcagaag agctggcgga gatcaccttg atggcggcag aaactgttcg
    38701 tcgttttggt attgagccgc gtgtggctct gttgtcgcac tccaactttg gttcttctga
    38761 ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg gtcagggaac gtgcgccaga
    38821 actgatgatt gatggtgaaa tgcacggcga tgcagcgctg gtggaagcga ttcgcaacga
    38881 ccgtatgccg gacagccctt tgaaaggttc cgctaatatt ctggtgatgc cgaacatgga
    38941 agctgcccgc attagttaca acttactgcg cgtttccagc tcggaaggtg tgactgtcgg
    39001 tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta acgccgatcg catcggtacg
    39061 tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg caaacccaac cgctgtaatt
    39121 cttttttaac tctcacgctt accctgaata ttcagggtaa gcggtttagc tgcaatat
//
LOCUS       DAZFRE010000038        38096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFRE010000038 DAZFRE010000000
VERSION     DAZFRE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.038"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            88..1308
                     /locus_tag="ACHKUI_002277"
     CDS             88..1308
                     /locus_tag="ACHKUI_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6598464.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1281..1910
                     /locus_tag="ACHKUI_002278"
     CDS             1281..1910
                     /locus_tag="ACHKUI_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6598465.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1911..3071)
                     /gene="ybdL"
                     /locus_tag="ACHKUI_002279"
     CDS             complement(1911..3071)
                     /gene="ybdL"
                     /locus_tag="ACHKUI_002279"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6598466.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3180..4268
                     /gene="hcxA"
                     /locus_tag="ACHKUI_002280"
     CDS             3180..4268
                     /gene="hcxA"
                     /locus_tag="ACHKUI_002280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6598467.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNHQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4278..4475)
                     /locus_tag="ACHKUI_002281"
     CDS             complement(4278..4475)
                     /locus_tag="ACHKUI_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6598468.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4588..6693)
                     /gene="cstA"
                     /locus_tag="ACHKUI_002282"
     CDS             complement(4588..6693)
                     /gene="cstA"
                     /locus_tag="ACHKUI_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6598469.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6874..7287)
                     /gene="entH"
                     /locus_tag="ACHKUI_002283"
     CDS             complement(6874..7287)
                     /gene="entH"
                     /locus_tag="ACHKUI_002283"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6598470.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7290..8036)
                     /gene="entA"
                     /locus_tag="ACHKUI_002284"
     CDS             complement(7290..8036)
                     /gene="entA"
                     /locus_tag="ACHKUI_002284"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6598471.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8036..8893)
                     /gene="entB"
                     /locus_tag="ACHKUI_002285"
     CDS             complement(8036..8893)
                     /gene="entB"
                     /locus_tag="ACHKUI_002285"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6598472.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTINAW
                     WKLLSREVK"
     gene            complement(8907..10517)
                     /gene="entE"
                     /locus_tag="ACHKUI_002286"
     CDS             complement(8907..10517)
                     /gene="entE"
                     /locus_tag="ACHKUI_002286"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6598473.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKLGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEAGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10527..11702)
                     /gene="entC"
                     /locus_tag="ACHKUI_002287"
     CDS             complement(10527..11702)
                     /gene="entC"
                     /locus_tag="ACHKUI_002287"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6598474.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11891..12847
                     /gene="fepB"
                     /locus_tag="ACHKUI_002288"
     CDS             11891..12847
                     /gene="fepB"
                     /locus_tag="ACHKUI_002288"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6598475.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(12851..14101)
                     /gene="entS"
                     /locus_tag="ACHKUI_002289"
     CDS             complement(12851..14101)
                     /gene="entS"
                     /locus_tag="ACHKUI_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6598476.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITMLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14212..15216
                     /gene="fepD"
                     /locus_tag="ACHKUI_002290"
     CDS             14212..15216
                     /gene="fepD"
                     /locus_tag="ACHKUI_002290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6598477.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15213..16205
                     /gene="fepG"
                     /locus_tag="ACHKUI_002291"
     CDS             15213..16205
                     /gene="fepG"
                     /locus_tag="ACHKUI_002291"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6598478.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16202..17017
                     /gene="fepC"
                     /locus_tag="ACHKUI_002292"
     CDS             16202..17017
                     /gene="fepC"
                     /locus_tag="ACHKUI_002292"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6598479.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17014..18147)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUI_002293"
     CDS             complement(17014..18147)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUI_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6598480.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLSLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLRPSLPVKKD
                     GPGKSIIVILSALIGGMVACGGVLLRHAMASRKQDAMMADHLV"
     gene            complement(18363..22244)
                     /gene="entF"
                     /locus_tag="ACHKUI_002294"
     CDS             complement(18363..22244)
                     /gene="entF"
                     /locus_tag="ACHKUI_002294"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6598481.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHITAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAASQDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
     gene            complement(22241..22459)
                     /locus_tag="ACHKUI_002295"
     CDS             complement(22241..22459)
                     /locus_tag="ACHKUI_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO6598482.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQSQ
                     SQASCQQWLEAHWRTLTPANFIQLQEAQ"
     gene            complement(22462..23664)
                     /gene="fes"
                     /locus_tag="ACHKUI_002296"
     CDS             complement(22462..23664)
                     /gene="fes"
                     /locus_tag="ACHKUI_002296"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO6598483.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQASLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23907..26147
                     /gene="fepA"
                     /locus_tag="ACHKUI_002297"
     CDS             23907..26147
                     /gene="fepA"
                     /locus_tag="ACHKUI_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6598484.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            26322..26981
                     /gene="entD"
                     /locus_tag="ACHKUI_002298"
     CDS             26322..26981
                     /gene="entD"
                     /locus_tag="ACHKUI_002298"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6598485.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVHALREYGLKGVPAIDHHRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTAGELTDNIITAAEHEQLAGCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLHYQIGNCQHQQMTISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(26983..27918)
                     /locus_tag="ACHKUI_002299"
     CDS             complement(26983..27918)
                     /locus_tag="ACHKUI_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6598486.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGAAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGIKLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(27911..28741)
                     /locus_tag="ACHKUI_002300"
     CDS             complement(27911..28741)
                     /locus_tag="ACHKUI_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6598487.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEGLKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALKELKDA
                     "
     gene            29104..30054
                     /locus_tag="ACHKUI_002301"
     CDS             29104..30054
                     /locus_tag="ACHKUI_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6598488.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDATDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            30405..31925
                     /locus_tag="ACHKUI_002302"
     CDS             30405..31925
                     /locus_tag="ACHKUI_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HFO6598489.1"
                     /translation="MFAGNKGFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVITQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAALAHEL
                     NTLPASVDSTMGVYFIPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALTPGTVADLKTLLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            32029..32964
                     /locus_tag="ACHKUI_002303"
     CDS             32029..32964
                     /locus_tag="ACHKUI_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6598490.1"
                     /translation="MKLRLTLLTAATLTAISFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKTANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            33011..33631
                     /locus_tag="ACHKUI_002304"
     CDS             33011..33631
                     /locus_tag="ACHKUI_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 family protein"
                     /protein_id="HFO6598491.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGGITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            33633..35176
                     /locus_tag="ACHKUI_002305"
                     /pseudo
     CDS             33633..35176
                     /locus_tag="ACHKUI_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            35194..36261
                     /locus_tag="ACHKUI_002306"
     CDS             35194..36261
                     /locus_tag="ACHKUI_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598492.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGDIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     EALGNTGFATLGSGTFLGVYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            36315..37112
                     /locus_tag="ACHKUI_002307"
     CDS             36315..37112
                     /locus_tag="ACHKUI_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6598493.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAARVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLAHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            37145..37930
                     /locus_tag="ACHKUI_002308"
     CDS             37145..37930
                     /locus_tag="ACHKUI_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6598494.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGGGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
BASE COUNT         9285 a        10237 c         9825 g         8749 t
ORIGIN      
        1 ataatgtgct ctatcttgct tcgtttatcc tataattagc ggattcatct cacgaatcac
       61 cccgtaacgt tcagagagcg tttttagatg tctatttata aaatcccgct tccactgaat
      121 attctggaag ctgcaaaaga acgtattacc tggacactta ataccctacc ccgtatctgt
      181 gtttcttttt cgggtggtaa agattccgga ttaatgctgc atctgacagc cgaaattgcc
      241 cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact gggaagcgca gttctcatgc
      301 actattaact atgttcagtc attacgtgag ttttacgccg atgtcatcga agagttttac
      361 tgggttgcgc tcccgcttac gacgcaaaat tccctttcac aataccaacc cgaatggcag
      421 tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct
      481 gactttttct ccttttacca gcctggtatg accttcgaac aatttgtgcg tgagtttgcc
      541 gaatggtttt cgcaaaagcg tccggcggcg atgatgatcg gcatccgcgc agatgagtcc
      601 tacaaccgtt ttgtcgccat cgccagttta aataaacaac gttttgccga tgataagccc
      661 tggaccaccg ccgcaccagg cggtcatagc tggtacattt accccatcta cgactggaaa
      721 gtggctgata tctggacctg gtatgctaac catcaacagc tctgtaaccc actgtataat
      781 ataatgtatc aggcaggcgt ccctctgcgt catatgcgaa tttgcgaacc ttttggcccg
      841 gagcaacggc aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg
      901 tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg ccggacatga taatcatttt
      961 tatggtcatc ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg
     1021 ctgctcaata gcatgccgga aaaaacggct gagcattacc gcaataaaat cgctatttat
     1081 ttgcactggt atcagaaaaa aggcatcgaa gttccacaaa cccagcaagg ggacattggc
     1141 gcgaaagata tcccctcctg gcggcgaata tgcaaagttt tactcaataa cgattactgg
     1201 tgccgggcat tatcatttag tcctacgaaa gcgaagaact atcagcgtta taacgaacgg
     1261 ataaaaggaa aacgtcagga atgggggata ctatgcaaca acgattaacg caggatttaa
     1321 cccaatttct cgccagcttg ccggaagatg atcgcatcaa agcgattaat gaaatccgca
     1381 tggcgatcca tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta
     1441 aaaacagcca acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa
     1501 aactgctgaa aaaatccatt gaaatagatg gttttaccca acccattgta gtcacgcata
     1561 cggataaaaa tgctctggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct
     1621 catcatcgtt aaaattacgc ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc
     1681 gtaatcaacg catcgccgcg actattcgtc ataatcgcgc tcgtggacgt caccaaatca
     1741 ctgccatgtc agaaatcgtc cgagaactca gccagttggg atgggacgat aataaaattg
     1801 gcaaagagct gggaatggac agcgacgaag tgttgcgcct gaagcaaatt aacggcctgc
     1861 aagagttgtt tgccgaccgt caattttccc gcgcctggac ggttaaataa ctacagctgg
     1921 cgcaggcgtt ctgctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc
     1981 agtttatgtg ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc
     2041 tcccgcgtca gccactggca aaactcaaca tcatccagcg tagatacggc gctgtaatcc
     2101 accagcaaaa agtatgtacc ttcgcacggt aaaatctcca gccgactttc atttaaggca
     2161 ttcaccagaa tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt
     2221 tctgcacgta gcatatcggc aagcgccaac tgtgccgggg tattcaccga aaaggtcaga
     2281 tactgatgca ctttgcggat ctcggcgctg atgggcgcag gcgcaacgca ataacccact
     2341 ttccagccgg tcatatgata ggtcttgcca aacgatgaca ccgccaccgc tcgctcacgc
     2401 aattgcggat gcgccagcac gctggcatgg ccctgttgtg aaaaattgat gtgctcgtag
     2461 acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg cctgccacag agcggcgaaa
     2521 tcagtctgct gccagacagt tgcactgggg ttatgcgggg tgttgaggat caccagtcgg
     2581 gtgcgctcgc ttaacaatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggcggttgc
     2641 agtgcaatac gtttcactat tccccccgaa agtgcgatgg cgggggcgta actgtcatag
     2701 ctgggatcaa aacaaatcac ttcgtcgcca ttgcgcacca atgcggttat cgccgcgtat
     2761 aacgcctccg tcgcccctgc cgtcacggtg atattgctgt cgacatccgg ttgatagcca
     2821 tacagacgtt ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggt
     2881 gcgtattggt ttgccccctg ggcaacatgg tacgccagcc gctcctgtaa atagcgcgga
     2941 ccatcaaaat caggaaagcc ttgcgaaagg ttaatggcct ggtgttgctg cgccagcgcg
     3001 ctcatccggg tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg
     3061 ttatttgtca tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg
     3121 ctagtatttg gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca
     3181 tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catcccggaa
     3241 gtttcaatca cctgcacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct
     3301 acggcgaacg cgccattgct gcggcacaaa ccaaactgcc gcctgcgttt gaactgccag
     3361 gggtaaagca tattttgttt cgtggtcatt gcagcgaaag cgatgtgcaa caactggcgg
     3421 cggagtccgg tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctactcgaca
     3481 ccgcgaaagc cctcgcccgc cgtctcggtc tgccgtttgt tgccgttccg acgatcgccg
     3541 ctacctgcgc cgcctggaca ccgctctccg tctggtataa cgatgccgga caagcgctgc
     3601 attatgagat tttcgacgac gccaatttta tggtgctggt ggaaccggag attatcctca
     3661 acgcgccgca acaatatctg ctggcgggga tcggtgacac gctggcgaaa tggtatgaag
     3721 cggtggtgct ggccccgcaa ccagaaacgt tgccgctaac cgtgcggcta gggatcaata
     3781 atgcgcaggc tattcgcgac gtcttgttaa acagtagcga acaggcgctg agcgatcagc
     3841 aaaatcacca gttaacgcaa tcattttgcg atgtggtgga tgccattatt gctggtggtg
     3901 ggatggttgg tggtctgggc gatcgtttta cgcgtgtggc ggcggcccat gctgtgcata
     3961 acggtctgac cgttttgccg caaaccgaga agtttctcca cggcaccaaa gtcgcctacg
     4021 gaattctggt gcaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg
     4081 cgtatcagcg ttttcatctg ccgactacgc tggcggagct ggaagtggat atcaataatc
     4141 aggcggagat cgacaaagtg attgcccaca ccttgcgtcc ggtggaatcc attcattacc
     4201 tgccggtcac gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta
     4261 aagcctgacg tacatattta gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc
     4321 tgccgctcac ggaaaaactc ctcgtaggtc atcggcgttt gatcgggatg gttaacccgc
     4381 atatgttcga catagttgtc gtaatcaggc ataccaatca tcagtttcgc cgcctgacct
     4441 aaatattttc cggcttttgc cagtgaatca aacatctaaa cctccggtaa tacaaatgcc
     4501 ctcctgaatt aagagggctt aattgctatc aacacgctcg ggatgagagt aaggggagcg
     4561 ctcagcacat tccccctcac tctgacttta gtgcgcgcct ttcgcctgcg ccacgatctc
     4621 ctcgacattt tccggcatcg gctcatatgg cgtttctttc gccgttggct tcggctcttt
     4681 caatgccgcc agtgccgtct taatcgagaa cagcgccaga accaccacga ccaccataaa
     4741 gaagatggtt aacccggcat ccagacggtt gttgaacacg agttgcgcca gctgtgactc
     4801 agtatactgc gacggaatat tgccgctgtc gatcattgcc tggaacttgt tagcaatcgc
     4861 caggaatccg actttcgcat ccgggctaaa cgctttctgc cagcctgcgg tcaaggtaca
     4921 aatcagcagc caggccgttg gtaccagcgc cacccaggcg taacgttgac gtttcatttt
     4981 gaacagcacc acagcacaaa gcatcagcgc catccctgcc agcatctggt tggcaatacc
     5041 aaacagcggc cacaaggtgt taataccgcc cagcggatcg accacgccct gatgcaggaa
     5101 gtagccccac gccagcacgc acagcgccgt tgccagcagg ttagcaggca gtgaatcggt
     5161 ccgtttcagg cccggagaca ccacgcccag cagatcctgc aacataaagc gcgcagcacg
     5221 cgtacccgca tccaccgccg tcagaataaa cagtgcttca aacagaatgg cgaagtgata
     5281 ccagaacgcc acatccatca tgccgcccag cgcaccgtgc agaatgtagg ccatccccac
     5341 cgccagggtc ggcgcaccgc ccgcacgaga aatgatcgac tgttcaccca cttcgctggc
     5401 aatctggttt agcgtatccg gggtaatcgc aaagccccag ctactcacca cctgcgcggc
     5461 agaagcaacc acatccgccg tccctgccgg agccagcacc gccatcgggc tgttcatggc
     5521 gaagtacaca cccggatcga tgatacaggc ggaaaccagc gccataatcg ccacgaagga
     5581 ttccatcaac atcccaccgt agccgataaa gcacgcctgc ccttcgttcg ccagcatctt
     5641 cggtgtagtg ccggaagaga tcagtgcatg gaagccagac accgcgccac aggcaatggt
     5701 gataaacagg aacgggaaca ggttaccggt ccataccggg ccagtgccat cgacaaattt
     5761 agttagcgca ggcatggtca gcgtcgggcg cataatcaaa atgcccaccg ccagaccgac
     5821 gatagtcccg attttcagga aggttgagag gtagtcacgc ggagccagca gcaaccacac
     5881 cggcagcacc gccgccacaa aaccgtaacc caccagcatc caggtcagct gcacgccggt
     5941 aaagtcaaag tacggtgccc acgtcgggct ttctgccacc cagccgccag agataatggc
     6001 gaaaataagg aacaccagac caatcaccga cacttcgcca atacgccccg gacgcaaata
     6061 gcgcaggtag atccccataa acagcgccag cggaatggtg aacgcaacgg tatacgttcc
     6121 ccacgggcta tgagtcaggg ctttaaccac gatcatcgcc agcacggcaa ggataatgac
     6181 catgatcata aagcaggcca ccagcgctat caccccggcg gttggcccca tctcttcttt
     6241 gaccagctca cccagcgagc gaccgtcacg gcgcgtggaa acaaacagca ccatgaaatc
     6301 ctgcaccgca ccggcgagaa ccacccctgc gagcagccag atcatccctg gcaggtaacc
     6361 catttgcgcc gccagtaccg gcccaaccaa cggacctgct ccggcaatgg ccgcaaaatg
     6421 gtgaccgaac agcacttttt tgtccgtcgg cacatagtcc agcccgtcgt tatggcgcac
     6481 cgctggcgtc atacgcgtcg ggtcaaccgc cagcacattt ttggcgatat acagcccata
     6541 aaaacggtaa gcgatcagat agatacagac cgacgccacc acaatccaca gcgcgttgat
     6601 ctgttcccca cgatttaaag caatgtatcc cagagcaaat gctcccatta cagagagcac
     6661 tgtccagacg aggtatttcc ctgatttgtt catagttgtt atccgtgtgc gtgtccatag
     6721 agatgttaca ttttgtttct ataacatctt tgtacaattt aacaacacag caaccatgta
     6781 aatcggagtt gaaaccaata tttaacttaa caatgttaac tcgatcactc cgttaaccga
     6841 attttcctgc aatctcattt tattctgtcg ggttcatccc aaaaccgctg tgcccagccg
     6901 acaggtgcag caacgccgcc cctgttcatc gaaaacgacg atttcccagc tctgattttg
     6961 tcgtccaaga tgcagcggtt ggcagacacc gcgcaccttt ccctcagaca ccgggcgatg
     7021 atgtgtggcg ttaagttccg tgcccaccac acactgtccg tcgcgggtca tcataaatcc
     7081 ggccatcgat cccagcgttt ccgccagcgc cgccgacgcg ccgccatgca gcaggccaaa
     7141 cggctgatga gtacgggtat caaccggcat ttcggcttcc agcacatcat cgcccagacg
     7201 ggtatacaca attcccagat gcgccaccat tgtgttatcg ctggtggcat tcagctcgtc
     7261 gagcgttaaa tggcgtttcc agatcatgct tatgccccca gcgttgagcc gccatcgacc
     7321 acaatatcct gtagggtgat atggctggcg agatcagagg cgaggaacaa aatcgtattg
     7381 gcaatctcct gcggacgggc gattttccct agcggaatgc cgagtttaaa ctgctcgcca
     7441 aagccgcgaa tacgctgctc ttcggcgtca tcgctaaccc acagcgtgcg ttgcatatcg
     7501 gtgtcggtgg agcccggcga aaccacatta cagcgcacgc cgctacccgc cagttccagc
     7561 ccgacgctca acgccaggct tttcagcgcc gctttcgacg cgccataagc actcatacca
     7621 atgcgcggcg tgtgtgcggc gtcggacgca acagtaacaa tcgccccgcc ccgctgacgg
     7681 cgaaactggt tcatggtctg ctgaaacagg ttaaacgcac cgccgacgtt gaccgcaaaa
     7741 gtctgctgcc agtcctcttt actgagctga tcggtcgcgc ccatgcgcaa aattcccgcc
     7801 gcattgacca gcacgtccag tcgctccgtt tcagctaaca gtcgctgaca tacctgcgcc
     7861 acctgcgcag catcggcaac atccattact tcggtcgcaa aaggatattg ctcctgagta
     7921 aacgcttgat caaaacctgt aactttcgcc cccgcctcaa caaatgccag cgccgtggca
     7981 tagccgatac ctttccccgc gccagttacc cagacatttt taccgctgaa atccattatt
     8041 tcacctcgcg ggagagtagc ttccaccagg cgttgatggt cgggtttttc gccagcatga
     8101 caaagtcgat atcgccatgc actttgcgcc agcgcgccgc cagcgccatc atgcgcaccg
     8161 aatccagacc gtagtcgatc aggttgtcgt catcgaaggg ttcatcggac tcgtccagca
     8221 acggcaggat cacctcacgc agcgccgctt tgctggcggg gataggtgct ggcagcaatt
     8281 cttcggtcat caccacccga ccagaacgcc cggccacata tttcagcgac atcaaatgct
     8341 cgtcacggct gaaatcggcc agcgcatccg ccaccataaa cggtttaata tcgcgcataa
     8401 atgcgtccgt tgcggtggtc atacagccaa tgtgggcata taccccggta ataatcagct
     8461 ggttacgtcc actctctttg agcatttgct ccagcggaga acgatgaaat gcgctgtagc
     8521 gccatttcac cagcaccgtg tcgtcggcat ctggcgtcag gcgatccacc accttttgct
     8581 gttccggcga acgggtcagg ccaggccccc acatatcatt caacagcgcc cgatcttcat
     8641 cgctctgctc tttcggctgg gcggtgtaat aaaccgggat attgtgctgt ttgcagtagt
     8701 cgcgtagtgc cgcaatattc gcgatcacct gctccatcat cgggcagttc tcaccccaga
     8761 agctgacaaa atagtcctgc atatcatgga ttaacaacgc ggcacgttgc ggttcaaagg
     8821 cccagtcgac tttattctgc ggaatatcat gagactccgg cagtgcgtaa gcctgtaatt
     8881 ttggaatagc catcgtgttc tctccttcag gctgatgtgc gtgacgccag ccactgacgt
     8941 aattgttttt tatcgacttt cccgaccgcc gtcagcggaa gtgaatctac acactccacg
     9001 cgatccggta atttaaattc ggcaataccc tgttcacgca ggaaacgacg cacctgcacc
     9061 gcgcgcagcg gctcttttac caccagataa gcacagcttt tttcgcccat cagctcatct
     9121 tccatgctca ccagtgcggc gtagatcacc gccggatggc gcagcagcag gttttcgatc
     9181 tcttcggcag cgatcttctc gccgccacgg ttgatctgat ctttctcgcg cccctgcaca
     9241 gtgatgtaac cctctggatc aatagagatc agatcgccgg aacagtaaaa accgttggca
     9301 tcaaaggcgc tggcattgtg ctgcgggctt ttgtaatagc cacggaaggt gtacggcccg
     9361 cgcgtcatca ggcgtccagc ttccccttgc ggcagtggat ttccttcggc atcggcaacc
     9421 catacttcat cgtccgggca cattgggtaa ccctgggtat ggataatttt ctccgcacta
     9481 tcatcaagac gggtgtagtt caccagccct tccgccatgc caaacacctg ctgcaactga
     9541 cagccaatct cagcgggaat acgcgccgca agggtggcag aaagacgggc gccgccgacc
     9601 tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc cttcagccag tgcctgcaac
     9661 cacaggctga ctgctggcgg caccagcgcg gtgacgttca cctgatgttt ttcaatcaat
     9721 gggaagcaaa gcgtggcgct ggggtcggca gccagaacga cagtgccgcc agcgagaaat
     9781 acccccaacg atcccggtga actcatggcg tagttatgag ccgccgggat cgcgcacagg
     9841 tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac gacgcacgct gtagtagtag
     9901 tcgttatgag tgcgcgggat cagtttcgga gtgccggtgg tgccgccgga aagctggaaa
     9961 taggccactt catcagcagg agatggcgtg gcagtaaaat cctcagccgg atggttaatc
    10021 gcatcctgca agttatgctc accgctgtcg ttgagcagct gcaccacgcg aatcgaagaa
    10081 tgctctgcaa caaatgtgtt gaggaaatca tccccgctaa atagcgcatg ttggcgatcg
    10141 gcaatcagta acgccggttc aatctggctg gcataggcgt tcagttcact acgctgatgg
    10201 ctgaacagcg ccagcaccgg cgcaacgccc agtttcagca gcgcgaaaaa ggtaatgtaa
    10261 agttcagcga cgttacccag ttgtaccagc gcggtttcac caagtttaat gccctgacgg
    10321 cgcaaactac aggcgaggtt atccgccgcc tgattcagct cccgataact caactgccgc
    10381 tcgccgtcga taaccgcgat gctgtcactc gcagcgtggc gagtcagaat gtcggtcagt
    10441 ggcaaatcct gccagtagcc tttttcccga tagcgacggg caaactcttc cggccagcgg
    10501 gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa aaacgttcaa catggtagaa
    10561 agtttgacgc ccgtttcgcg ccactcaccc aacggtgagg acgcaggcac aatccccgct
    10621 ccggcaaaca gacgcacctg attttcccgc agcttcgcgc agcggatggt caccacccat
    10681 tcaccgttac cttcgctgtc acaccaaccc acaatgccgc caaacagttc gcggtcgaac
    10741 ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg ggaaaccgct cagcgcgggg
    10801 gtcggatgca gcagacaagc caaagtcagt gcgttttctt gcgaattcgc tttaccttca
    10861 aagggagttg cgagatgcca cagcgtcggc gtggtaatca attgtggaga ggagggaacg
    10921 tgtaactcac tactgcgttt gcgcagtacc tctttcatcg cctgagtcac cagttcatgt
    10981 tcatggcgat ctttttctga cgccagcaga cgattacccg cttcgcgatc gagcacttca
    11041 tccggctgac gacgcgcgga accggctaac ggaatggagc taaaacgctc gccgtcttta
    11101 cgtagcagca gttccgggct ggcccccagc agaacgccac catcagccaa cggaacatgg
    11161 aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca gcaatacgcc actatcaatg
    11221 gcggcgtcag tagtgatatc aatcaaccgt gacaacacca ctttgtcgac ctgcggcgtg
    11281 gcggtaagtg cggcagcacg ggcaaccatc tgttcaaacg tggtttgttc cggaatggcc
    11341 tgacgttcca ccacgttcag cgactggctg cgggtgaaac ggcgtgcgga agcttgtttt
    11401 tcctgacggg agaacgactg ccaggattcg ggaatataca gcgacgaagg ctgacgtgga
    11461 tcgaagggaa tcgccccgac catcaccgga tttttgatgc cctgcgcttt ggcatcggca
    11521 aacaacgcgg cgagtttttg ctggaaggga ctgtcgggcg aatccccgtt cacagccggt
    11581 tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc ggtacggcga cataaagaaa
    11641 aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt cctcagccag tgacgtatcc
    11701 atatcatcct ccacaaaatg ataaaggctt taataatgat tatcatttat attttcggtc
    11761 gctaacctaa aagcaaacgc gcactatgtc aactcttgag gtaacgcaat ctgcaactcg
    11821 gggttgcatc tggttgtggt tacaatgaaa atgagaagca ttaacttcat taataacagg
    11881 aagctgactt gtgagactcg ccccgctcta ccgcaacgcc cttctattaa caggactttt
    11941 gctttcagga atagccgcag ttcaggccgc cgactggccg cgtcagatta ctgacagccg
    12001 tggcactcat accctggaaa gccagccgca gcgtatagtt tccaccagcg tcaccctgac
    12061 cggctcactg ctggcgattg atgctccggt gatcgccagc ggcgcgacca cgccaaataa
    12121 ccgcgtcgcg gatgaccagg gctttttacg ccagtggagc aaggtggcga aagaacgcaa
    12181 actgcaacgg ctctatatcg gcgaaccgag tgcagaagcc gttgccgcgc aaatgccgga
    12241 tctgatttta attagcgcaa ccggcggcga ttcggcgctg gcgctgtacg atcagctttc
    12301 taccatcgcc ccaacattaa tcatcaatta cgacgacaaa agctggcagt cgctgttaac
    12361 gcagcttggc gaaattaccg ggcatgagaa acaagcggca gagcggattg cacagtttga
    12421 taagcaactg gcggcggcga aagagcaaat caaattaccg ccgcagccgg tcactgccat
    12481 cgtctatacc gccgctgcac acagtgccaa tctctggacg ccggaatcag cacaagggca
    12541 gatgctggaa caactcggct ttacgctggc gaagttgccc gcaggcttaa acgccagcca
    12601 aagccagggc aaacgccatg acatcattca gcttggtgga gaaaatctgg ctgcagggtt
    12661 aaatggcgag tcactattcc tgttcgccgg tgatcagaaa gacgccgatg ctatttatgc
    12721 taatccgctg ctcgcgcacc tgcctgcagt acaaaacaag caggtttatg cgctgggaac
    12781 cgagacgttc cgcctggatt actacagcgc catgcaagtg ctggatagac ttaaggcgct
    12841 gttttaagca ttaactgtcg gacgctgtca cctgcggcgg cgtctggcga aaacgtcgca
    12901 actccaccag caccagcagc aacaacacgc cgataatcaa caaaccaaaa ccgctcgcgc
    12961 ttgcggaagc aaccggtgtc atcatcgctc ccaaaccgcc cagcagcgcc gcgcctatgg
    13021 catcgcccgt cacgttttgc gccgtccaca aaccgttaat ccgccctaac atcgcttccg
    13081 gggtttgcgt ttgcagcatt gtatattgca gcaacgaact aaccgcgctc agccagccga
    13141 acagcgccag acaaaccacg cctaaaatcc acatcggcat caggccaaac agaccaatgg
    13201 cgaggaacgc ccccaatgtg gagagcaaca tcaacagacc tggccgtacg ctatgcgcca
    13261 gttttccact ggttaacgcg ccaatagccg caccgagcgg gattgccgca tagagaaagc
    13321 caatttgcgc cgccgacatc tgccagttgt cagccagcgc cggatacagt acccgcaccg
    13381 cgctcgccat cgtcaataaa ccccccagca acgcaatccc tcccaccagc gggctggcga
    13441 gtagaaaacg aaatcctgcc agtaatgatt tcaacggatg ctcacgcggc tgcggcggtg
    13501 gtggcaacgc cggaaggctt aacaacggta gcatggtaat aaatgtgccc gccgccgcca
    13561 gcccgtagtt ccaggctacg cctccggtcg ccagcagtaa accgccaatc atgggcgaaa
    13621 tcaccgatcc cagacgcaca gtcaacatag tgatcgcccc ggcctgcatt aagttttcac
    13681 gccctaccag tgcaggtgtc gccgccagta gcgccgtaac gccaagtgat gcgaaaaaac
    13741 catcccataa accaagtaaa tagattgcca gcaatgacgg ctccggcagc agcgcgttca
    13801 ggcacagtcc aatgaagcca atgccacagg tgccgcgcgc cagcaaaatc acttttttac
    13861 gctcatagcg atccgccagc acaccgccga ccatcaggcc aacaaacatc gcgccgccgg
    13921 tcagcgtcac cgaaagcccc acctgccagg tggaatgcgt catcatctgg atctgcaccg
    13981 gcaccgcgac gccgagcaaa cccagagaaa caattgagat aaaacgagcg aggaatactg
    14041 cgcgaaacgc cgggtgcgtt ttcaacaggc tgaggttaag cagccaggat tgtttattca
    14101 ttacaatgcc ttgccatcaa atattttaaa tacctattct taggctgcac atgctaacat
    14161 atccaaataa gatcgataac gataattaat ttcattatca tggaagttcg tatgtctggt
    14221 tctgttgccg tgacacgcgc cattgccgtg cccggattgc tgttattact gattatcgcg
    14281 acggcattaa gcctgctcat tggggcaaaa tcactccccg cttccgtagt gctggaggcc
    14341 ttctccggca cctgccagag cgccgactgc accatcgtgc tcgacgcacg actgccgcgc
    14401 acccttgccg gtttactggc aggcggtgcg cttggccttg ccggggcgtt aatgcaaacc
    14461 ctcacccgaa acccacttgc cgatcctggt ttgcttggcg tgaacgccgg tgccagtttt
    14521 gccattgtgc tgggtgcggc gctgtttggt tactcttccg cacaggaaca actggcgatg
    14581 gccttcgccg gggcgctggt ggcctcgttg attgttgcct ttaccggcag tcagggcggc
    14641 gggcagttaa gtccggtgcg tttaaccctg gcaggcgtgg cgctggcggc ggtgctggaa
    14701 gggctgacca gcggcatcgc cctgcttaat cccgacgtct acgatcaact gcgtttctgg
    14761 caagccggtt cgctggatat tcgcaatcta cataccttaa aagtggtgct gatcccggtg
    14821 cttaccgctg gagcaactgc gctattactg agccgcgcgc tgaacagttt aagccttggc
    14881 agtgacaccg cgacggcact gggcagtcgc gtagcgcgca cacagttgat tggtctgctg
    14941 gcaattaccg tgctttgtgg tagtgcgacg gcggtagttg gcccgattgc ctttattggc
    15001 ctgatgatgc cgcacatggc gcgctggctg gtaggtgccg atcatcgctg gtcgctgccc
    15061 gtcacgctac tcgctacccc tgccctgctg ctgtttgccg atatcattgg gcgggtgatt
    15121 gttcccggcg aactgcgcgt ttctgtagtc agtgccttta ttggcgcacc agtgctgatc
    15181 ttcctcgtac gacgtaaaac gcgaggtggc gcatgattta cgtctctcgc cgattaatta
    15241 tcacctgtct gctgttgcta atagcttgtg tgatggcagg cgtgtgggga ttacgtagcg
    15301 gtgccgtcac gctggaaact tcgcaggtat tcgccgcgtt gatgggcgac acgccgcgca
    15361 gtatgacgat ggtggtcacc gaatggcgtt taccacgcgt gctgatggcg ctgttgattg
    15421 gcgcggcgct gggcgtcagc ggcgcgattt ttcagtcgct gatgcgtaac ccgctcggca
    15481 gccctgacgt aatgggcttt aacaccgggg cgtggagcgg cgtgctggtg gcgatggtgc
    15541 tgtttggtca ggacctgacg gctatcgcgc tggcagcaat ggtaggcggc attgtcactt
    15601 cgctgttggt ctggctgctc gcctggcgta acggtatcga cacctttcgg ttgattatta
    15661 tcggtatcgg tgttcgcgcc atgctggtgg cctttaatac ctggctgttg ctgaaagcgt
    15721 ctttagaaac ggcgctaaca gcgggtttgt ggaatgccgg atcgctcaat ggcctgacgt
    15781 gggcaaaaac ctcgccttcc gcacccatca ttatattaat gttaattgcc gccgccttgc
    15841 tggtgcggcg aatgcgcttg ctggaaatgg gcgatgatac cgcatgtgcg ctgggcgtca
    15901 gcgtcgaacg ttcgcgactg ttaatgatgc tggtcgccgt agtgcttacc gcagctgcca
    15961 ctgccctagc ggggccaatt tcctttattg ctttagtcgc gccacacatt gcccgccgca
    16021 tcagtggcac cgctcgctgg gggctgaccc aggcggcgct gtgcggggcg ttgttactgc
    16081 tggtggccga tctctgcgcc caacaactgt ttatgccgta tcaacttccg gttggcgtcg
    16141 ttaccgtcag cctcggcggt atttacctta tcgtcttgtt aattcaggag tctcgcaaaa
    16201 aatgaccgaa tcagtagccc gtttgcgcgg cgaacagtta accctcggat atggcaaata
    16261 taccgttgcg gaaaatctga ctgtagaaat acctgatggt cacttcacgg caattatcgg
    16321 gccaaatggc tgcggtaaat ccacgttact gcgtacctta agccgcctga tgacgcctgc
    16381 tcacgggcat gtctggctgg atggcgagca cattcaacat tacgccagta aagaggttgc
    16441 acgccggatt ggtctgttgg cgcaaaatgc taccacaccg ggcgatatca ccgtgcagga
    16501 gctggtggcg cgtgggcgtt acccgcatca accgctgttt acccgctggc ggaaagaaga
    16561 tgaagaggca gtcacaaaag cgatgcaggc cacgggaata actcatctgg cggatcaaag
    16621 cgtggacacc ctttctggcg gacaacgcca gcgagcgtgg atcgctatgg tgctggccca
    16681 ggaaacggca attatgctgc tcgatgaacc cacgacctgg ctggatatca gtcatcagat
    16741 tgatttgctg gaactgttaa gcgaactgaa ccgcgagaaa ggctataccc tggcggcggt
    16801 gctgcacgat cttaatcagg cctgtcgtta cgccagccat ttgattgcat tgcgggaagg
    16861 gaaaattgtt gctcagggag caccgaagga gattgtcact gctgaactga tcgagcgcat
    16921 ttatggtctg cgctgcatga ttattgacga tccggttgcc ggaacgccgc tcgtggtgcc
    16981 gctcggacga acggcacctt caaccgcaaa gatttaaact aagtggtctg ccatcatggc
    17041 atcctgtttt ctggatgcca tcgcatggcg caacagcaca ccaccacaag ccaccatccc
    17101 gccgatcaac gcggaaagga tcacaataat cgacttaccc ggaccgtctt tttttactgg
    17161 caatgacggt cttaactgat atttaaacgg cgtaaaattc acatcgttga tatttgcttt
    17221 tgttaactgc tcaacaagat actggcgatt acgtaattcg ccgttcagtt ccgcaacgtc
    17281 agtaaccgct ttttcaattt ccagtttgcg ttcaataccg tcagcaccga gagaaataga
    17341 gaaatcgggg tcatctttaa cggcctgacc attactgtat acgggctttt taattcctgc
    17401 cgcgttggca atgtcgagtg aataattgag gcgctgaatg tttgcatcaa gttgattttt
    17461 cgttttaatg cgatcctgag ccagtttttc tttttcaaac tgggttttga tctccagttt
    17521 attacggacg ttttctatcg actctttcac caccaacgca gagatataat cgatataccc
    17581 gctcaaaacg gtctgcgcct cttcactggt tggcgcggta aaacttagcg tccaggaggt
    17641 atacagtgac ggttcatctt ttttcttact ggcattgtca tcaaccgctt tcattttttc
    17701 gctcaatgcg acaattgcgc gatgcaaatc cagttcgtcg attttcgcct cttttaattg
    17761 gtccattaca taaggtgatg agcgcaggta ctcttctagt aagctaacag actgaaactt
    17821 cttgataaac aggttaaatg cttctgtacg atcaattttg atatccagat ccagcacacg
    17881 aagcttagtg aatgttttct ccagctcttg ccactgaaca ggttctggag gcgtgacaac
    17941 cgccgcgcta gtccattttt gcggcaggat gaaagagatc agcaagcctg cgcaggcaaa
    18001 cgcaaaaacg accgccatga ccgttttttt ggcccgccat aaaacttcaa ttagactaag
    18061 taaatcaatt tcattattac tgggcgacgc cagaggatac tcgggaaaat gagcttcact
    18121 tccctgttta atattcagtg atgacatggg gtataacctg aaaatgagtc caataagagt
    18181 aaatctccac ggcccccact ctaccagttg gtgagattta tccgaagctg aagtttgtaa
    18241 attactccca ctgaatgcaa cattcactct atttcagaga aatacccacc aaatttataa
    18301 attaaacaaa tgataaaccc ataattattt tctgtaatta tgggtttata gataatataa
    18361 atttacttat ttagcgtcgc acgaataatc ggcccaattt tttcaaacgc caccggagag
    18421 ataatatcca catgcgcaca atcctgacga tagatatcca gctcagcaat ccacggtgcc
    18481 caggcctgtt caggagtcat accttcctga agcgtgcgtt cggcaacaaa tagcgtcgct
    18541 ttaccatcaa acgctacgct atgagctgtc gtcagcaggc gcacagcatc agcgtagttg
    18601 ccttcaatgg tggtaaacaa ctccgttgaa gtacttccct gctgtgccgc caggaaggct
    18661 tcgcgctcgc ggttaatctc cgccagcact tccgggtcca gaccattggc ttctttttcc
    18721 tgccagtttt gcgtttctgg cggccaggta tccagcaagc caagaaatgc cacctgttcg
    18781 ccacgggcac gcagtcgcgc cgcaatgccc tgcgccagcg taccgccaag tgaataaccc
    18841 agcaggtaat atgggccgtg cggttgttgt tcaagtaagg ttgccagatg cgcttcgcag
    18901 acttcatcct ggcttgccgc cgtctgcatg gggccatgag ggcgcggcga ctgaatgccg
    18961 ataatcgacc attgtggatc gagataacgc gagagcacgc tgaactgcca ggcaaaaccg
    19021 gatgccggat ggaaacaaaa cagcgtcggg ccattacctt cacgcaacgg cagaatggtt
    19081 tcgaatccca tgcgctgggt gctgtcttct tcaccatcaa taatcgttgc cagtttggcg
    19141 acagttgacg cgaccatcac ctgacccggc gtcacctgac gggcaaactg ccgacttaac
    19201 tgcgctgcca gtttcattgc cagtagcgaa tgaccgccaa gcgcaaagaa atcagcatcg
    19261 gcatcctgca cgtcacaacc cagtaacgac gagaatgccg cggcgataat cgtttcactg
    19321 cccgctttcg gcgcacgccc cggcgcttgt gccttcagtt caggcaacgg taaggctttg
    19381 cgatccagct tgccgttggc gctaagtggt aactgtggaa gttgcagcag aaccaccggt
    19441 accatatgtg gtggtaatgt ttcacgaagc tgcgcctgta atgcgctggt atccaacggc
    19501 agacctgatt gcgacaccag atagcccacc aactgacgcg catcaccacc agtggctgcc
    19561 gcctggttaa tcacacaggc gtgggtaacg gcctgttcga catccggcag tgcctgcatc
    19621 acgcgatcga tttcgcccag ttcaatacgc tgcccgcgaa tttttagctg atcgtcactg
    19681 cgcccgaggt actccaccgc gccgttatcc agccagcggg caacgtctcc ggtacggtac
    19741 atccgttcgc caggggcaaa aggatcggca ataaagcggc tggcggtcag atcgggtcgt
    19801 ccaagatacc cctgcgccag ttgaataccg gtgagataga gatctcccgc cacacccggc
    19861 ggcaccggat gcatcatcgc atcgagaatg cgcaggcccg tattccacac cggataacca
    19921 atcggcacac tgctgccgcg cacctctgcc agttcctcgc caaaagccgg ataccaactg
    19981 acatctaccg ccgcttccgt cgggccatat agattatgca acggcgcgcc ggttaactgt
    20041 tgccattcgc ggcataaatc agccggtaag gcttcaccac tacagaaaac ctgtttcaac
    20101 gtcgcgcaac tctggcgagc ggtttgcggc gtcagcgatg caacaaatgc cgccagcatc
    20161 gacggcacaa agtgcgtggt cgttacgcca tattcggcaa agaattgctg catagcgagc
    20221 gggtcgcggt gcgcttccgg ttcagccatc accagttttg ccccggcaat aaatggccag
    20281 aaaaactccc acaccgagac atcaaaactg cacggcgttt tttgggcaac gacatcttcg
    20341 cctgtaagcg ggtaatggtt ttgcatccaa agcaggcgat taacgatagc cgtctgcccg
    20401 accattaccc cttttggtct gccggtggaa ccggaggtga agatgatata agccgtgtgg
    20461 tgcggttgtg aaagctgcag cggcgcactg ccctgcggtg taagcggggc gttatagcaa
    20521 aggctggtta aattagggat atcgctaaag cgcggcagtt gatcgtcagt ggtgattaac
    20581 agcgacggac gcgcatcttc cagcatcatt ttcaggcgat cgtccggata gccggtatcc
    20641 aacggtaacc aggccgcacc agcttcaact atcgcatgta gcgccagggt caaaaagacc
    20701 gagcgcggta gagccaccgc cacgctgtcg cccggtttaa cgccgcgctc acgcagcaga
    20761 ttcgccagcg ccaccacctg ctcgcgcatt tcccgatagc tgaactggta acgcgcatct
    20821 gccagcgccg gagcatccgg tgtttttgcc gcttgttctg ccaccagcgc gctcagcgtg
    20881 gtttctggaa tctcaatctg agtggcgttg atctgcgcca gctgcgcata ctcacctggc
    20941 agcataatat cgacatcgcc gcacaacagc gccggatccg cggcgaactg cgcaatcagc
    21001 attttcaggc gttcagcgtg ttgaattaac gttggctcat cgtaacgctg tttattggcg
    21061 aggatttcaa tactcaaatc accgtgttca tccgggaaca gggccagttc aaggtcatta
    21121 accggaccgg ttgccagggt atgggtttgc gcctgaacac caggaatatc cagttggtaa
    21181 tcaaatacct tgatattgag taccggaccg aacagcggtt cctctcctgc cgctcgcccg
    21241 ctgtcacgga caatttgttc ggcatcgtaa cgttgatggc gacgcatttt tttcagttga
    21301 gccgccagtc gggttgccag ttgcggcagc gtttcttgcg ccgtaatgtg aatacccaac
    21361 ggcaaaacgt tgagcacggg tccggtagcc gtcagcgccg ccgagcccag tcgacgcata
    21421 aagataaatc cggcagcgta gtccatgcgg ttgcacaatc gccccagcca caaagctgcc
    21481 aacgcaaggg ctaaatcggt acgctgcaca cctgaaagtt gcgtagccag ctggcggaat
    21541 tccccgtcgg taaattccag tttcaggcgc aaaatatctg ccgaagcgct gcgccccggt
    21601 aaaggtgccg gagaaagtga cgcgggcggt ggcagttgac gacgctgttc cgcccagaat
    21661 gccgcatcac gctgccaggc ttcactttcg cggtactgct gatactcttc cactacatca
    21721 gcgaaaggcg taaacggcga agcaggcgtt ggttcgccac gcagccatgc gcagtaaata
    21781 ttggcgatct ggcgtgtaat ggccgggaaa ctgaagccat cgaccagcaa atggtgataa
    21841 cgctgatacc agtaccagcg gttatccgcc acctgtatca gctgatgaaa gaccagtggt
    21901 ttaccactat cgacgcgcag atcttgttgc aaatccgcct gcattaatgc ctgcgcagta
    21961 ccgtgcggat caatgttggt tcgcaggtcg ataatttctg gcagttcgaa cgtctgcgca
    22021 tcatcgaccc actgccagac ttcgccgtta tcttccgtaa aacgcatccg caacgtatct
    22081 gcttgcgcta gtccggcaac caccgcgcgg gccagtagtg gcgcatcaac ctctccggtt
    22141 aactcaacgt aatgcgccac gctccaggcg gagggtaaat ccgacagttt ttctgccatc
    22201 cagatgccgg gctgtgcggc gaccaaaggt aaatgctggc tcattgtgcc tcctgcaact
    22261 ggatgaaatt cgccggtgtc agagtacgcc agtgggcttc cagccactgc tggcaggacg
    22321 cctgtgactg cgactgacac acaacgtccc agcctgccgg taaggcgcat tgttgcggcc
    22381 acagactgaa ttgcccctgc gcattgcgca aaatgtaaaa cgctccctgt ggatcatcga
    22441 agggattact gaatgccata ttcaactcct gtcatggaaa agtggttgcc agaggtcgat
    22501 tagcccctgc atcaagccac cgcgccaaca aagcgcatca tgtccgccgt caacctgacg
    22561 ccagaaaatg gattctttta gggggtgtaa ttgcgcatac agcgcctgat tggcctgcat
    22621 gatcatcggc tcgcgaacac ccgcttccag cacaatgcgc agaccttcgg cgctaacttc
    22681 accagtattt agctgttcaa gtaacatgcc ctcttggtgc ccgccccgat gcggccacca
    22741 gtacgaacct gactggctta atacacagcc aaagcgttca ggccagtgca gtccggcata
    22801 cagcgcggaa agcccaccaa aactctgtcc ggcgaccacg gtgcgatcgg cgcgatcgct
    22861 aaaaggggca atagctttca cctggggtaa taactcttgc tgtactgcca gccagaaatc
    22921 cgcattacac ggcagttcgt gggcgcggtg cgtggtgtcg atagcgtcga tcaacacata
    22981 cacggcgggc ggaagttgcc gacgatgggt cagcgaagtc agcgcaggcc agacgggcat
    23041 actctgcgcc caaaattcgc catcaagcaa aactgccagc gggcgttctt ctgctgttgc
    23101 atcgccggtg gtaaaaatcc atacacggcg tgaatttttc aaccgttcac ttttccagat
    23161 aatttctttg gcaggcgttt ctggcgcttg cggacaatcc catcccggtt gcagagacgc
    23221 ttgcggcatt tcgagtgcag aaacagcgtg ccctcgcccg cctttccagc tttgtggatt
    23281 cagcggatcg gctatcgcct ggggtaatag ttttcgccag ccttcgcgta attcgaggcg
    23341 atcgggggat ggtgcagaaa aaatgtcatc gcgttcagta ggaataaagc agtagctgcc
    23401 acgccagttg gcattgagtt gtgtcgtcca ctgccaaaca tcggttcctg caattcgctg
    23461 catcgaccgg ggctggctgt tctgatggtg atcggtcacg ccagtgatat agacccatac
    23521 gcgctttatc gtcgagtatt cttcagaacc ctggggatca cgccaccaga aagtgacctc
    23581 aaacatttcg tcattcagac gttgccattc cgggccatgt ttcgactgcc accagctctc
    23641 acttcctact tttaacgccg tcacaccata accccatgtt tactgtgcaa ttttttattg
    23701 attgcagaaa tatattgata atattattga taactatttg catttgcaat agcgtaatgg
    23761 cgcgccgtgg gaagcgcgga cattaattaa tcagctgcac tgcgtgtctt tcaggatcaa
    23821 aggttttcgc ggtagcggga tgcgtcgtgt tgatgacgac catgcccgac agttgcaatt
    23881 cgtggcaaaa atgcaggaat aaaacaatga acaagaagat tcattccctg gccttgttgg
    23941 tcaatctggg gatttatggg gtagcacagg cacaagagcc gaccgatact cctgtttcac
    24001 atgacgatac tattgttgtt accgccgccg agcagaactt gcaggcacct ggcgtttcga
    24061 ccatcaccgc agatgaaatc cgcaaaaacc cggttgcccg cgatgtatcg gagatcattc
    24121 gtaccatgcc tggcgttaac ctgaccggta actccaccag tggtcagcgt ggtaataacc
    24181 gccagattga tattcgcggc atgggtccgg aaaacacgct gattttgatt gacggcaagc
    24241 cggtaagcag ccgtaactct gtgcgtcagg gctggcgtgg cgagcgcgat acccgtggtg
    24301 atacctcctg ggtgccgcct gaaatgattg aacgtattga agttctacgt ggtccggcag
    24361 ctgcgcgtta tggcaacggc gcggcgggcg gcgtggttaa catcattacc aaaaaaggca
    24421 gtggtgagtg gcacggctcc tgggatgctt atttcaatgc gccagaacat aaagaggaag
    24481 gtgccaccaa acgcaccaac ttcagcctga ccggtccgct gggcgacgaa ttcagcttcc
    24541 gcttgtatgg caacctcgac aaaacccagg ctgacgcgtg ggatatcaac cagggccatc
    24601 agtccgcgcg tgccggaacg tatgccacga cgttaccagc cgggcgcgaa ggggtgatca
    24661 acaaagatat taatggcgtg gtgcgctggg acttcgcgcc tctgcagtca ctcgaactgg
    24721 aagcgggcta cagccgccag ggtaacctgt atgcgggtga tacgcagaac accaactctg
    24781 acgcttacac tcgctcgaaa tatggcgatg aaaccaaccg cctgtatcgc cagaactact
    24841 cgctgacctg gaacggtggc tgggataacg gcgtgaccac cagcaactgg gtgcagtacg
    24901 aacacacccg taactcgcgt attccggaag gtctggcggg cggtaccgaa gggaaattta
    24961 acgaaaaagc ggcacaggat tttgtagata tcgatcttga tgacgtgatg ctgcacagcg
    25021 aagttaacct gccgattgat ttcctcgtta accaaacgct gacgctgggt acagagtgga
    25081 atcagcaacg gatgaaggac ttgagttcca acacccaggc actgaccgga acgaataccg
    25141 gcggtgctat tgatggcgtg agtgccaccg accgtagccc atattcaaaa gcagaaattt
    25201 tctcgctgtt tgccgaaaac aatatggagc tgactgacag caccatcgta acgccggggc
    25261 tgcgtttcga tcatcacagt attgtcggca ataactggag cccggcgctg aacatatcgc
    25321 aaggtttagg cgatgacttc acgctcaaaa tgggcatcgc ccgcgcttat aaagcgccga
    25381 gtctgtacca gactaaccca aactacattc tctacagtaa aggtcagggc tgctatgcca
    25441 gcgcgggcgg ctgctatctg caaggtaacg atgacctgaa agcggaaacc agcatcaaca
    25501 aagagattgg tctggagttc aaacgcgacg gttggctggc gggcgtgacc tggttccgta
    25561 acgattatcg caataagatt gaagcgggct atgtagctgt agggcaaaac gcagtcggca
    25621 ccgatctcta tcagtgggat aacgtgccga aagcggtggt tgaaggtctg gaaggatcgt
    25681 taaacgtacc ggttagcgaa acggtgatgt ggaccaataa catcacttat atgctgaaga
    25741 gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc ggagtatacg ttgaactcaa
    25801 cgctgagctg gcaggcacgg gaagatttgt cgatgcaaac gaccttcacc tggtacggca
    25861 aacagcagcc gaagaagtac aactataaag gtcagccagc ggttggaccg gaaaccaaag
    25921 aaatcagtcc gtacagcatt gttggcctga gcgcgacctg ggatgtgacc aagaatgtca
    25981 gtctgaccgg cggcgtggac aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc
    26041 agaccacggg cgatctggca ggagccaact atatcgcagg tgccggggcg tatacctata
    26101 acgagccggg acgtacgtgg tatatgagca ttaatactca cttctgaagc taacgtcaga
    26161 ttgttgacaa agtgcgcgta gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    26221 aaaaatcttg ccaattcaat atattgcagg accacgtagg cctgataagc gtagcgcatc
    26281 aggcagtttt gcgtttgtca tcattctcta atatggttga catgaaaact acgcatacct
    26341 ccctcccctt tgccggacat acgctgcatt ttgttgagtt tgatccggcg agttttcgcg
    26401 agcaggattt actctggctg ccgcactacg cacaacttca acacgctgga cgtaaacgta
    26461 aaacagagca tttagccgga cggattgccg ccgttcatgc tttacgagag tatggtttga
    26521 aaggcgtacc tgcaattgat catcatcgcc aacctgtctg gcctgcgggg gtatacggca
    26581 gcattagtca ctgtgggact acggcattag ccgtggtatc tcgtcaacca attggcattg
    26641 atattgaaga aattttttct gcacaaaccg caggggaatt gacagacaac attattacgg
    26701 cagcagaaca cgagcaactc gcaggctgcg gtttagcctt ttctctggcg ctgacactgg
    26761 cattttccgc caaagaaagc gcatttaagg caagtgagat ccaaactgat gccggttttc
    26821 tgcactacca aattggcaat tgtcagcatc aacagatgac gataagcggg ccgaatggcg
    26881 accatatcgt gcagtggtta gtaaggggta ataatgttat taccttgtgc ctggcagata
    26941 aacacccgca cacagccgca tgcgggttat ctggcgggta attcaaagca tttcccgtgc
    27001 ctgagctgca attccggcag catcaaatcc atgatgctga cgcaaccacc cacgatccgc
    27061 cgctgcggca tattctccat ccataatgcc tgcgcgtttt aatttgatcc ccgttccggc
    27121 ctctgccaac acttccgcga ccaggctacc aaggccgcca ttgacgttat gctcttcaac
    27181 cgtcatcact gctttgcatc cttttaacgc tgccagcaat gctttggtgt cgcatgggcg
    27241 aatcgacggt acgctgacaa ctgtagccgc gataccagac tgcgctaaaa gttctgcggc
    27301 atcgaccact tcatgaacgg tagagcctgt ggctaccagc gccagttcgc tgccctcgcg
    27361 caaagtcacc acggcacctg gtacaaatcg gtagtcctca tcgtggattt ccggcagggc
    27421 tttaccgtcc atacggatat aaacaggccc ctgatagcga agagcataat caatcacctg
    27481 acggcactcc cgaggtgaag acggggcaaa gatttgaata ttgccgaaac cacgcataac
    27541 cgcaatgtca tcaatcgcat gatgcgtgct cgccagcggt ccataactcg ccccggcatt
    27601 cagaccaaac aattttacgt tggtaaagtt gtagcagaca tcaaccttaa tttgctcgtt
    27661 agcgcgagcg ataagaaatg gcgcggcatt gcaggtaaca gccaccttgc cgcccagcgc
    27721 cagcccggca gcggcaccaa ccatgctctg ttcggcaata cccacattca ccagtcggtc
    27781 aggaaattgc ttaataaagg gagcaatttt tgccgtagac gttgaatcag ccactacagg
    27841 caccaaatcg atcccctcat ttacggcatc gataaatgcc tgaaccatca catttgccag
    27901 atgttcactg ttacgcatct ttgagttcct tcagtgcctg ttcaatttca tcgcctttcg
    27961 gcacccggtg atgccattcc gcacgccctt gtataaagga aatgcctgcc cctttagtgg
    28021 tgtgggcaat caccacattc ggtttgcctt cctgtttaag cccttcaagc gtcgcgacaa
    28081 ccgaagccat atcattgccc tgacattcgg taacctgcat accgaatgcc agccatttct
    28141 ctgccagagg atcggtgttc atgatttcgc gcgttggccc ggctaattgc agattatttt
    28201 tgtcattaat aatgaccagg ttatcaagcc cgtaatgcgc cgccgccagt gcagcttccc
    28261 agttactgcc ctcagccagc tcaccatcgc cagtaattaa gaagatacga cgacggcttt
    28321 gacttttttt cgccgccagc gcaagtccta cagccacagg gaaaccatgt cccagtgctc
    28381 cggtattcag ttcaatacct ggcgtttttt gccgaaccgg atgaccgggt aagtgtgaat
    28441 ttggctgctg ataagtggaa agccattcca gtgggaagaa tcctgcttcc gccagcacgc
    28501 agtagtaaca tccaacagca tggccttttg actgaatgta tatatcccgg tcatcgtcgt
    28561 gaaggcgatc cggggcgaca ttgagaatac gaaaatagag tgcggtcagt aattcaacct
    28621 gagagagatc ggccccagtg tgtccacctg ccgggctatt ggcatttaag cgcacgatat
    28681 ggcggcgtac cgcccgcgct ttacgctcaa gggtctggat atcatagcta tagggattca
    28741 tttaatacct ctgaatttat tttcaacatg gaatatttat tccaccgggg actaaaaaaa
    28801 ccagaaatcc cagggcgttg cgcggcaaga cttacgctgc tgcatcatac tcaggaacat
    28861 tctgcctcct tacaccgtca gttacatgtc cgggtaatca tcagttgacc tgatatcatt
    28921 ttgaatatat attcaataat gattctttat tcaattatgc cgcctgtttt ttatttttgt
    28981 ccatcgctga ttttaaggat tggttaaagt tagaactcca tcacacttaa ctgtcttctt
    29041 ttttcgcgta tgataaaagc ctctaagata agggcggtac gttaatgatt tacggggaaa
    29101 tatatggcta agcaggatga acagcggcta ctggtgaaga tcgccactct ttactatctt
    29161 gaggggcgaa aacagtctga catcgcccaa cttctctcgc tttctcagtc atttatctcc
    29221 agagcgctaa cccgttgcca gaaagaaggc gtggttaaaa tcagcgttgt ccagccgtca
    29281 aatatctttc tcaatctgga aaaaggcatt gaagagcgtt acggcattaa acaagcgatt
    29341 gttgtcgata ccgaagatga tgccactgac cataccatta aacgtgctat cggctctgcc
    29401 gccgcgcact atctggaaac gcgtttaaga ccaaaagatt tcattggcgt ctcttcctgg
    29461 agttctacga ttcgcgcgat ggtcgatgaa gttcacgccc aaaacttaaa agccagcggt
    29521 gttattcagc tgctgggcgg cgtggggcca aacggcaatg tgcaggcaac tattttgaca
    29581 cagaccctgg cccagcatct gaattgcgaa gcctggttgc ttccttctca aagcattgaa
    29641 ggttcggtag aagagaaaaa acgcctggtg gcaagccagg atgtggctga cgttatcgcc
    29701 agatttgacg acgtcgatat cgccattgtc ggcatcggta tccttgaacc ctcgcagttg
    29761 ctaaaaacct cgggcaacta ttatcacgag gatatgttac aggtactggc ggatcgcggt
    29821 gctgtgggcg atatctgcct gcactactac gataaacatg gtcagcccgt gttgcaggac
    29881 gatgaagatc cagtgattgg tatggcactg gataaaatca aaaaatgccc gaatgtggtg
    29941 gcactggcgg gcggcaaaga caaagttgcc gccatcaaag gcgccatgca gggtggttat
    30001 attgatgttt tgatcactga tttccccacc gccagaatgc tgattgcgga ttaatcctct
    30061 cctccctttc ttctgcccgc ctttgcgaaa aaatcttctc gcaaaggcgc ttgcttaaaa
    30121 agcctttaat atcaataaac tgaaaggatt caacaatcct gtttctcgag gctttctcac
    30181 cctgtcacca cgcttctaag tgtgatagcc agcacatttg atgatttttt ctctagctca
    30241 ggaagaataa tctggctaag gtataaataa ataatcaata tcgaatatat attcacacct
    30301 taacacaaaa tgtcacagca gggcctgatg aagtaataac gtgaccatga ggaacgcctc
    30361 atctccttcc acgccaggat gcaatgtgac tcaggagact acctatgttt gcaggaaata
    30421 aagggttcgt tattgcgttg gatgagggta ccaccaatgc aaaagccatc gcacttgatg
    30481 gtttcggtaa cgtggtggcg aagttttctc aaccactggc tattcagacg ccacgtgaag
    30541 gctgggttga gcaatccggg gaagcgttaa tctctgcctc gcttgatgtt attacccagg
    30601 ccatcaacca cgtgggggca gaaaatgtag cggctctggc tatcagtaat cagcgtgaaa
    30661 cggctatcgg ctggtatcgt cagagtggaa aaccactcaa tgcggcgatt acctggcaat
    30721 gcacgcgcag tgcagagttt tgtgaaacac ttcgtcgcga gggcaaagag cagcaaatta
    30781 aaacgactac aggcctgcct atcgccccgc tattttccgc ttccaagatg cgttggctac
    30841 tggaatctac acctgagggt gtgcaacttg ccgaccaggg tgaaatttgt ctcggcacca
    30901 ttgatgcctg gttgctatgg aacttgactg ctggcgattc attttgctgc gactactcta
    30961 acgccgcgcg aactcaatta cttaacctga aaacaggcga gtgggatgag gaaatgctgg
    31021 cgttgtttca catcccacgc gctgcattgc cagaaattaa accttccagt ggcctgttcg
    31081 gttatacccg aggacttaaa acaattccgg atggtattcc cgtcatggcg atggtgggcg
    31141 attctcacgc cgctcttttt ggtcatgcgt tgggagaagc aggatgtgtg aaggcgacct
    31201 atgggaccgg ttcttccgtc atggcccccg tcaactctgc tcagtgtgat atcgccacac
    31261 ttgccacaac cattgcctgg catgacggcg aaaatattat ttacggtctg gaaggaaata
    31321 tcccccacac cggcgatgcc gtggcatgga tggctgacag caccggttta agtgaactga
    31381 cagacgcggc actggcccac gaactgaata ccctgccagc ctcagtggat tcgactatgg
    31441 gcgtttattt tattcctgct ctcacaggcc tgggcgcgcc atggtgggat gacagtgctc
    31501 gcggcgtggt gtgtggctta agccgtggcg taaagcgtgc gcatttgatt cgcgccgcac
    31561 tggaatcaat cacttaccaa attgcagatg tagtagaagc catgcaacag catgatgatt
    31621 ttcacttatc ggcgttaatg gtggatggcg gcccgaccaa aaatgactgg ttgatgcaat
    31681 atcaggcgga tctgctgggt tgcccggtga tgtgcagtga tgtgccagaa ctttcggcca
    31741 ttggtgcggg tctgctcgcc cgcaaagcgc ttacccccgg caccgttgca gatttaaaaa
    31801 cgttattaac tgagcacagt gaatttaaac cggatatggc gcgtcaccag cgactgcaaa
    31861 agagatggca ggagtggcgt catgcagtga acagaacatt atggaaaccg gcatcgtcaa
    31921 tttgacgaac aagactaacc aacggaagga gaataccggc ccataaataa ttcaggcttt
    31981 acgttgtttc accgtaccag acaataacaa cacagagaga acattgtcat gaaattacgt
    32041 ttaacactgt taaccgctgc gaccctgact gctatttctt tttctgccca tgcggtagaa
    32101 aaaggaacca ttatgattat ggtcaattca ctggataatc cttattacgc ctcggaagcg
    32161 aaaggcgcca gtgaaaaagc ccagcaactg ggatataaaa cgaccattct ttctcatggt
    32221 gaagatgtca aaaaacagaa tgaactgatc gatactgcaa ttggtaaaaa agttcagggc
    32281 attattttag ataatgctga ctctactgcc agtgttgcag ccattgaaaa agcaaaaaaa
    32341 gcaggtattc ccgttatatt aattaatcgc gaaatacctg tggatgatgt tgcactggtg
    32401 caaattaccc ataataactt ccaggctggc tcggaagttg ccaacgtatt tgtcgagaaa
    32461 atgggagaga aaggaaaata tgccgaactc acctgtaatc ttgctgataa taattgcgta
    32521 actcgctcga aatctttcca ccaggttatt gaccagttcc ctgaaatggt tagcgtggcg
    32581 aagcaagatg ccaaaggcac gttaattgat ggcaaacgaa taatggacag tattctgcaa
    32641 gcccatcccg atgttaaagg ggtgatttgt ggtaatggtc cggttgcact gggagctatc
    32701 gcagcactga aaaccgccaa tcgcaatgac gttattgttg tcggcattga tggcagtaac
    32761 gatgagcgcg atgccgtgaa agccgaatca ttgcaggcta ccgtgatgtt acaagcgcaa
    32821 gccattgccg cgcagggagt taccgatctg gataattacc tgcaaaaagg agttaaacct
    32881 gccaaacagc gggtgatgtt ccgcggcatt ctgataacca aagataacgc agataaagtc
    32941 caggatttca atatcaaatc ctgatcccag gtcgttgcgc cctcacaatg agggcgcaga
    33001 ggagaatatt atggataaaa aagtctggct ggcgctggca attctgagcc tgggtggatg
    33061 tcgcatcgtc tcgcagcagg aacttgccga tctcaaatcc ccgcctaatc ctcatatggc
    33121 aaatatcgat cagacctggc agaaaaacat cgtgccgcag gtggtagaaa atgcgcgtcc
    33181 ggttgcggag ttaatggcgg cgctacaggc agaaaaagat gtcgatgctg cctgtaaaac
    33241 gctgggctat cgttctcagg aagaaaaccc gtgtattttc tacgttaagg tcgaaggtgg
    33301 catcaccaat attgatgcga aatcccgtag cggaaaaatg accatcacag atatcagcgg
    33361 cacaaatatt gtcgtgcaga cggggccaac tcttcgcggc acgcttctgc gagatgcata
    33421 taaaggtgca agctatgagc attttaacga tcaggtttta tttggtgaat atggtcgtgc
    33481 tatcaatgag caagccatca atatgattca gtcggcaaag cttaaaaccg gggaaacggt
    33541 tcaggtttat ggcgtatttt ctgcatggga tattccgcaa actatcccgg atataacgcc
    33601 tgcgaaaata gccagggcgg gaggcgagta atatgttgcc gcaagaaatt gacacccccc
    33661 agactgatga atcacctgta atcatagaaa cgcatgactt atcgcgtatt tatccgggtg
    33721 tggtggcgtt agataacgtg aattaccgtg tctatcgcaa taaagtaaat gtgcttattg
    33781 gtgaaaacgg tgcgggtaaa tccacaatga tgaaaatgct ggcgggggtt gaaacacctt
    33841 cctcaggcaa gatcattctt gatggtaaag aagttaccct aaattctacc catcaggccg
    33901 aaaaacatgg aattagtatc atttttcagg agctgaattt attcccgaat atgaatgtca
    33961 tggataatat tttcatggcg aatgaatttt tcagaaaggg aagattaacg aaaaatatca
    34021 atatgaatta gccaaatctc tattagaaag actggaactg aatgttgatc cctataccca
    34081 actgggagag ctaggtattg gccatcagca actggtggag attgcccgtg ccctttctaa
    34141 agatacccgg gtactgatta tggatgaacc gacatcggcc ctcagccagt cggaagtaaa
    34201 agtccttttc aatgtgatcg cacaactgaa gcgccgtggt gtcaccatca tttatatctc
    34261 ccatcgtctg gaagagttga tggagattgg cgatttcatc accattttcc gtgatggtcg
    34321 ctttattagt gaacgcgccg tgagcgatgc cagtattccg tggatcatcg agcaaatggt
    34381 cggtgataag aaaaaacatt tcgattatca accagctaca caaggtgaca ctgtactggc
    34441 ggtacagggg ttaacggcgc tgcatcccag cggtggttac aaactcaatg atgtgacatt
    34501 cagcctgcat aaaggcgaag ttatcggcat ttacggcttg ctgggcgcag gacgtactga
    34561 actatttaag ggactggtgg gcctgatgcc ctgtcagagt gggaagatcc agcttaatgg
    34621 tgaaaccatc gacaaatgcc atttccaggc gcggctcaaa aaggggctgg cgttggtgcc
    34681 agaagatcgc cagggcgaag gcgtcgttca gatgatgtcg attcagtcca acatgacgct
    34741 atccgatttc agcctgcaag gttttcgccg tgcctggaaa tggctgaatc cgcaaaaaga
    34801 gagcgccagt gtgaaagaga tgatcctgca actggcgatc aaagtcagcg atgccgaact
    34861 ccctattacc tcactcagcg gcggtaacca gcaaaaagtg gtacttggaa aagcgttgat
    34921 gacccagcca caggtggtgt ttcttgatga acccacacgc ggcattgatg ttggcgcaaa
    34981 aaccgacgtc tatcagctgg tgggaaaaat ggcacaacag gggctggcag tcatgttctc
    35041 ttcttcagaa ctcgatgaag tcatggcact ggcagatcgc attctggtga tggctgatgg
    35101 ccgtattacc gctgatctac cccggggcgg ggtcacccga gagaaactga tcgcggcttc
    35161 aacacctcaa gattaatcag gctggaattt cttatgaatc agaaatatat gatctatatg
    35221 tacctgttga aagccagaac gtttatcgct ctgctgttag tgatcgcctt ctttagcgtg
    35281 atggtgccta acttcctgac ggcctctaac ctgctgatta tgactcaaca cgttgcgatt
    35341 accgggctgc tggcaatcgg aatgacgctg gtgatcctga caggcggcat cgatctttcc
    35401 gtcggcgcgg tagcggggat ctgcggaatg gttgccgggg ctctgctaac aaacggcctg
    35461 ccattgtgga gcggcgatat tattttcttc aacgtgccgg aagtgatcct ctgcgtggcg
    35521 gtatttggca ttttagtggg actggtgaat ggtgcggtaa tcacccgctt tggtgtcgcg
    35581 ccctttatct gcaccctcgg tatgatgtac gtcgcccgtg gctctgccct gctgtttaat
    35641 gatggcagca cttaccccaa tctcaacggc atggaggcac tgggcaacac gggtttcgcc
    35701 acccttggtt ctggcacgtt tttaggcgtt tatctgccaa tctggctgat gattggcttt
    35761 ttgattttag gctactggat cacgactaaa acgccgctcg gtcgttatat ctatgccatt
    35821 ggtggcaatg aatctgccgc gcgactggca ggcgtaccca tcgtaaaagt caaagtgttc
    35881 gtctatgcgt tctccggatt atgcgccgca tttgtcggtt tgattgttgc ctcgcagctg
    35941 caaaccgctc acccaatgac cggcaatatg ttcgaaatgg atgctatcgg tgccacagtt
    36001 ctcggtggaa ctgcgctggc aggtggtcgg ggacgtgtct ctggctcgat tatcggtgcc
    36061 tttgtcattg tcttcctcgc cgatggcatg gtgatgatgg gtgtcagtga cttctggcaa
    36121 atggtcatta aaggcctcgt tatcgtcacc gccgtggtag tcgatcagtt ccagcaaaaa
    36181 ttgcaaaaca aagtcatttt gatgcgtcgc catgaagaaa agatggccgc cgcacctgcc
    36241 gtaaaaccgc tttccagtta attaacatca ttccgccggg cattaaattg cccggcttat
    36301 acaggggaaa aaatatgcaa cgtaattttc agggtaagac tgtagttatt accggggcat
    36361 gccggggaat tggggccggg atcgctgaac gcttcgcccg cgatggcgca aatctggtga
    36421 tggtttctaa cgctgcacgc gtgcatgaaa cggctgagca actgcgccaa tgttatcagg
    36481 ctgacatttt gtcattagaa gtcgatgtga cagatgaagc gcaagtacag gcactctatg
    36541 aacaagcggc tgcacgtttt ggcagcatcg atgtttccat tcagaatgca ggcattatca
    36601 ccatcgatta ttttgatcga atgccggctg ctgatttcga aaaagtgctg gcggtgaaca
    36661 ctacagcagt ctggctctgc tgccgggaag cagccaaata tatggtgaaa cagaatcacg
    36721 gctgcctgat caacacctct tctggccagg gacgtcaggg gtttatctat accccacact
    36781 atgcagccag caaaatgggt gtgatcggca ttacgcaaag cctggcgcac gagttagcgc
    36841 agtggaatat cactgttaac gccttctgcc ctggtattat cgaaagcgaa atgtgggact
    36901 acaacgaccg cgtatgggga gaaattctca gtaccgatga caagcgttat ggcaaaggcg
    36961 agcttatggc ggaatgggtg gaaggtattc cgatgaagcg tgccggtaaa cctgaagatg
    37021 ttgccggact ggttgccttc ctcgcctctg acgatgcgcg ttatctcacc ggtcaaacca
    37081 tcaacatcga cggcggcctg attatgtcgt aatgcccctg cgggctacta agagagatat
    37141 caaaatgatc gcaaaagatt ttgctcaaca attgtttgat ctccacgatc gcgtcgcctt
    37201 tgtcactggc gcaggtagcg gtatcggaca aatgatcgct ctggctttag ccagcgcagg
    37261 cgcacgagtg gtgtgttttg atctacggga agatggcgga ctcaaagaga cggttaatca
    37321 tatcgaagca cttggcggac aagccttgta ttacacgggc gatgttcggg aattgagaga
    37381 tcttcgcgca gcagtcgctc tggcaaaaac acattttggt cgtctggata ttgccgtaaa
    37441 cgccgctgga atcgctaacg caaacccggc gctggagatg gaaagcgagc aatggcaacg
    37501 cgttatcgat atcaacctga ctggtgtgtg gaattcctgt aaggcggaag ctgagttaat
    37561 ggtagagaac ggaggcggtt cgattataaa tattgcgtca atgtccggga ttattgtcaa
    37621 tcgtggtctg gaacaggccc actacaacag ttccaaagcc ggcgtgattc atttatctaa
    37681 aagtcttgcg atggaatggg tgagtaaggg gatccgcgtg aactcaatca gcccaggata
    37741 taccgcgaca ccaatgaata cacgacctga aatggtacat cagactcggg agtttgaaag
    37801 ccagacgccg attcaacgga tggcgaaagt ggaagagatg gcgggtccag cgctgttcct
    37861 tgccagcgat gcggcgtcgt tctgcacggg tgtggatctg gtggtcgatg gtggctttgt
    37921 ctgctggtaa aaatggtttc gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca
    37981 ggtgccttac caccctgacc tgacaaccgg atatgcgggg attactcccc gcacagcggc
    38041 tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa tattacgttc ctttac
//
LOCUS       DAZFRE010000039        38075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFRE010000039 DAZFRE010000000
VERSION     DAZFRE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.039"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..209
                     /locus_tag="ACHKUI_002309"
     CDS             <1..209
                     /locus_tag="ACHKUI_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598495.1"
                     /translation="QDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCLLL
                     FHPFCCIRTQNGAFLHAKRLAQGQ"
     gene            253..807
                     /locus_tag="ACHKUI_002310"
     CDS             253..807
                     /locus_tag="ACHKUI_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6598496.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(783..1040)
                     /locus_tag="ACHKUI_002311"
     CDS             complement(783..1040)
                     /locus_tag="ACHKUI_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6598497.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1037..1855)
                     /gene="aroE"
                     /locus_tag="ACHKUI_002312"
     CDS             complement(1037..1855)
                     /gene="aroE"
                     /locus_tag="ACHKUI_002312"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6598498.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTISRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            complement(1860..2432)
                     /gene="tsaC"
                     /locus_tag="ACHKUI_002313"
     CDS             complement(1860..2432)
                     /gene="tsaC"
                     /locus_tag="ACHKUI_002313"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6598499.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2437..2979)
                     /locus_tag="ACHKUI_002314"
     CDS             complement(2437..2979)
                     /locus_tag="ACHKUI_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6598500.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            complement(3008..3481)
                     /gene="smg"
                     /locus_tag="ACHKUI_002315"
     CDS             complement(3008..3481)
                     /gene="smg"
                     /locus_tag="ACHKUI_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO6598501.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3453..4577)
                     /gene="dprA"
                     /locus_tag="ACHKUI_002316"
     CDS             complement(3453..4577)
                     /gene="dprA"
                     /locus_tag="ACHKUI_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6598502.1"
                     /translation="MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4707..5216
                     /gene="def"
                     /locus_tag="ACHKUI_002317"
     CDS             4707..5216
                     /gene="def"
                     /locus_tag="ACHKUI_002317"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6598503.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5231..6178
                     /gene="fmt"
                     /locus_tag="ACHKUI_002318"
     CDS             5231..6178
                     /gene="fmt"
                     /locus_tag="ACHKUI_002318"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6598504.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            6230..7519
                     /gene="rsmB"
                     /locus_tag="ACHKUI_002319"
     CDS             6230..7519
                     /gene="rsmB"
                     /locus_tag="ACHKUI_002319"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6598505.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSMLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7541..8917
                     /gene="trkA"
                     /locus_tag="ACHKUI_002320"
     CDS             7541..8917
                     /gene="trkA"
                     /locus_tag="ACHKUI_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6598506.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQESVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9047..9460
                     /gene="mscL"
                     /locus_tag="ACHKUI_002321"
     CDS             9047..9460
                     /gene="mscL"
                     /locus_tag="ACHKUI_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6598507.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(9457..9675)
                     /gene="arfA"
                     /locus_tag="ACHKUI_002322"
     CDS             complement(9457..9675)
                     /gene="arfA"
                     /locus_tag="ACHKUI_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6598508.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(9731..10156)
                     /gene="zntR"
                     /locus_tag="ACHKUI_002323"
     CDS             complement(9731..10156)
                     /gene="zntR"
                     /locus_tag="ACHKUI_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6598509.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10167..10535)
                     /locus_tag="ACHKUI_002324"
     CDS             complement(10167..10535)
                     /locus_tag="ACHKUI_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6598510.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            complement(10642..11025)
                     /gene="rplQ"
                     /locus_tag="ACHKUI_002325"
     CDS             complement(10642..11025)
                     /gene="rplQ"
                     /locus_tag="ACHKUI_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6598511.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11066..12055)
                     /gene="rpoA"
                     /locus_tag="ACHKUI_002326"
     CDS             complement(11066..12055)
                     /gene="rpoA"
                     /locus_tag="ACHKUI_002326"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6598512.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12081..12701)
                     /gene="rpsD"
                     /locus_tag="ACHKUI_002327"
     CDS             complement(12081..12701)
                     /gene="rpsD"
                     /locus_tag="ACHKUI_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6598513.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12735..13124)
                     /gene="rpsK"
                     /locus_tag="ACHKUI_002328"
     CDS             complement(12735..13124)
                     /gene="rpsK"
                     /locus_tag="ACHKUI_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6598514.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13141..13497)
                     /gene="rpsM"
                     /locus_tag="ACHKUI_002329"
     CDS             complement(13141..13497)
                     /gene="rpsM"
                     /locus_tag="ACHKUI_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6598515.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13644..13760)
                     /gene="rpmJ"
                     /locus_tag="ACHKUI_002330"
     CDS             complement(13644..13760)
                     /gene="rpmJ"
                     /locus_tag="ACHKUI_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6598516.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13792..15123)
                     /gene="secY"
                     /locus_tag="ACHKUI_002331"
     CDS             complement(13792..15123)
                     /gene="secY"
                     /locus_tag="ACHKUI_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6598517.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15131..15565)
                     /gene="rplO"
                     /locus_tag="ACHKUI_002332"
     CDS             complement(15131..15565)
                     /gene="rplO"
                     /locus_tag="ACHKUI_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6598518.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15569..15748)
                     /gene="rpmD"
                     /locus_tag="ACHKUI_002333"
     CDS             complement(15569..15748)
                     /gene="rpmD"
                     /locus_tag="ACHKUI_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6598519.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15752..16255)
                     /gene="rpsE"
                     /locus_tag="ACHKUI_002334"
     CDS             complement(15752..16255)
                     /gene="rpsE"
                     /locus_tag="ACHKUI_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6598520.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16270..16623)
                     /gene="rplR"
                     /locus_tag="ACHKUI_002335"
     CDS             complement(16270..16623)
                     /gene="rplR"
                     /locus_tag="ACHKUI_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6598521.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16633..17166)
                     /gene="rplF"
                     /locus_tag="ACHKUI_002336"
     CDS             complement(16633..17166)
                     /gene="rplF"
                     /locus_tag="ACHKUI_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6598522.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTVRALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17179..17571)
                     /gene="rpsH"
                     /locus_tag="ACHKUI_002337"
     CDS             complement(17179..17571)
                     /gene="rpsH"
                     /locus_tag="ACHKUI_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6598523.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17605..17910)
                     /gene="rpsN"
                     /locus_tag="ACHKUI_002338"
     CDS             complement(17605..17910)
                     /gene="rpsN"
                     /locus_tag="ACHKUI_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6598524.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(17925..18464)
                     /gene="rplE"
                     /locus_tag="ACHKUI_002339"
     CDS             complement(17925..18464)
                     /gene="rplE"
                     /locus_tag="ACHKUI_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6598525.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18479..18793)
                     /gene="rplX"
                     /locus_tag="ACHKUI_002340"
     CDS             complement(18479..18793)
                     /gene="rplX"
                     /locus_tag="ACHKUI_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6598526.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(18804..19175)
                     /gene="rplN"
                     /locus_tag="ACHKUI_002341"
     CDS             complement(18804..19175)
                     /gene="rplN"
                     /locus_tag="ACHKUI_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6598527.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19340..19594)
                     /gene="rpsQ"
                     /locus_tag="ACHKUI_002342"
     CDS             complement(19340..19594)
                     /gene="rpsQ"
                     /locus_tag="ACHKUI_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6598528.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19594..19785)
                     /gene="rpmC"
                     /locus_tag="ACHKUI_002343"
     CDS             complement(19594..19785)
                     /gene="rpmC"
                     /locus_tag="ACHKUI_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6598529.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19785..20195)
                     /gene="rplP"
                     /locus_tag="ACHKUI_002344"
     CDS             complement(19785..20195)
                     /gene="rplP"
                     /locus_tag="ACHKUI_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6598530.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20208..20909)
                     /gene="rpsC"
                     /locus_tag="ACHKUI_002345"
     CDS             complement(20208..20909)
                     /gene="rpsC"
                     /locus_tag="ACHKUI_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6598531.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(20927..21259)
                     /gene="rplV"
                     /locus_tag="ACHKUI_002346"
     CDS             complement(20927..21259)
                     /gene="rplV"
                     /locus_tag="ACHKUI_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6598532.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21274..21552)
                     /gene="rpsS"
                     /locus_tag="ACHKUI_002347"
     CDS             complement(21274..21552)
                     /gene="rpsS"
                     /locus_tag="ACHKUI_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6598533.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21569..22390)
                     /gene="rplB"
                     /locus_tag="ACHKUI_002348"
     CDS             complement(21569..22390)
                     /gene="rplB"
                     /locus_tag="ACHKUI_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6598534.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22408..22710)
                     /gene="rplW"
                     /locus_tag="ACHKUI_002349"
     CDS             complement(22408..22710)
                     /gene="rplW"
                     /locus_tag="ACHKUI_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6598535.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22707..23312)
                     /gene="rplD"
                     /locus_tag="ACHKUI_002350"
     CDS             complement(22707..23312)
                     /gene="rplD"
                     /locus_tag="ACHKUI_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6598536.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23323..23952)
                     /gene="rplC"
                     /locus_tag="ACHKUI_002351"
     CDS             complement(23323..23952)
                     /gene="rplC"
                     /locus_tag="ACHKUI_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6598537.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(23985..24296)
                     /gene="rpsJ"
                     /locus_tag="ACHKUI_002352"
     CDS             complement(23985..24296)
                     /gene="rpsJ"
                     /locus_tag="ACHKUI_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6598538.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24534..24953)
                     /gene="gspB"
                     /locus_tag="ACHKUI_002353"
     CDS             complement(24534..24953)
                     /gene="gspB"
                     /locus_tag="ACHKUI_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO6598539.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            complement(24955..26424)
                     /gene="gspA"
                     /locus_tag="ACHKUI_002354"
     CDS             complement(24955..26424)
                     /gene="gspA"
                     /locus_tag="ACHKUI_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO6598540.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     AKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            26604..27419
                     /gene="gspC"
                     /locus_tag="ACHKUI_002355"
     CDS             26604..27419
                     /gene="gspC"
                     /locus_tag="ACHKUI_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6598541.1"
                     /translation="MPTLPFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NEQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            27403..29355
                     /gene="gspD"
                     /locus_tag="ACHKUI_002356"
     CDS             27403..29355
                     /gene="gspD"
                     /locus_tag="ACHKUI_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6598542.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSLGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            29365..30846
                     /gene="gspE"
                     /locus_tag="ACHKUI_002357"
     CDS             29365..30846
                     /gene="gspE"
                     /locus_tag="ACHKUI_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6598543.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            30843..32039
                     /gene="gspF"
                     /locus_tag="ACHKUI_002358"
     CDS             30843..32039
                     /gene="gspF"
                     /locus_tag="ACHKUI_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6598544.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            32049..32486
                     /gene="gspG"
                     /locus_tag="ACHKUI_002359"
     CDS             32049..32486
                     /gene="gspG"
                     /locus_tag="ACHKUI_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6598545.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            32494..33003
                     /gene="gspH"
                     /locus_tag="ACHKUI_002360"
     CDS             32494..33003
                     /gene="gspH"
                     /locus_tag="ACHKUI_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6598546.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVIVADGQITPFSLLMANAGTGKPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            33000..33377
                     /gene="gspI"
                     /locus_tag="ACHKUI_002361"
     CDS             33000..33377
                     /gene="gspI"
                     /locus_tag="ACHKUI_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6598547.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            33370..33957
                     /gene="gspJ"
                     /locus_tag="ACHKUI_002362"
     CDS             33370..33957
                     /gene="gspJ"
                     /locus_tag="ACHKUI_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6598548.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            33950..34933
                     /gene="gspK"
                     /locus_tag="ACHKUI_002363"
     CDS             33950..34933
                     /gene="gspK"
                     /locus_tag="ACHKUI_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6598549.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPVQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLNTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            34948..36111
                     /gene="gspL"
                     /locus_tag="ACHKUI_002364"
     CDS             34948..36111
                     /gene="gspL"
                     /locus_tag="ACHKUI_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6598550.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKFSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLSILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHHELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            36108..36569
                     /gene="gspM"
                     /locus_tag="ACHKUI_002365"
     CDS             36108..36569
                     /gene="gspM"
                     /locus_tag="ACHKUI_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO6598551.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            36569..37246
                     /gene="gspO"
                     /locus_tag="ACHKUI_002366"
     CDS             36569..37246
                     /gene="gspO"
                     /locus_tag="ACHKUI_002366"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO6598552.1"
                     /translation="MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(37275..37751)
                     /gene="bfr"
                     /locus_tag="ACHKUI_002367"
     CDS             complement(37275..37751)
                     /gene="bfr"
                     /locus_tag="ACHKUI_002367"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6598553.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(37823..38017)
                     /gene="bfd"
                     /locus_tag="ACHKUI_002368"
     CDS             complement(37823..38017)
                     /gene="bfd"
                     /locus_tag="ACHKUI_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6598554.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         9644 a         9846 c         8946 g         9639 t
ORIGIN      
        1 ttcaggattt tttctctttg ttgttacgac catcttgtga agtgtttcac gttgtcgtct
       61 caacggaggc gcattatagg gatcccaatt ttttgcacaa gtactttttt gatctttttt
      121 tctgtttgtt gctttttcac cctttttgct gcattcgcac acaaaacggt gcttttttgc
      181 atgctaaaag acttgcacaa ggccaataat gcacccaaag tcattagtaa atcatttatt
      241 gttgaggtga gtatgtctga tgttttacgc ccataccgcg atctttttcc acaaatcggt
      301 cagcgcgtaa tgatcgacga tagcagcgtc gtgattggtg acgttcgttt ggctgatgat
      361 gtggggatct ggccgctcgt tgtgattcgt ggagatgtac attatgtaca gatcggagca
      421 cgcaccaata tccaggatgg cagtatgttg catgtcactc ataaatcctc gtacaaccca
      481 gatggcaacc cattaaccat tggcgaagat gtcactgttg gtcacaaggt gatgctccac
      541 ggctgcacca ttggcaatcg agttttggtt gggatgggct caattttact tgatggcgca
      601 atagtagaag atgatgtgat gattggtgcg ggtagcctgg tcccacaaaa taaacggctg
      661 gagagcggat atctgtatct cggtagcccc gtcaaacaga tccgcccgtt aagtgatgaa
      721 gagaaggctg ggttacgcta ttccgcaaat aactacgtga aatggaagga cgaatatctg
      781 gatcaaggta accagaccca accttgatca tcttcacttt gttcctgaat taagttttcc
      841 gcttcttctt ccaggtccca gcgatgctga cgaaaactcg ctaaccactg ttctggcgta
      901 tctccagtaa agcgatacgc cagactctcg ccagagatcg cacatgtcag ttgcatacca
      961 ttcacgagag cgggaaaaca tacacatctt ttattctcgt cccactcttc cctatccgga
     1021 aactggatgg cctgattcac gctgacaatt cctgttgcaa tagtttgatg accggttcta
     1081 catcaggcag aacgccatgc caaagaagga aggcatgagc cgcctgtgcc accagcattc
     1141 ctaaaccatc agcattacgc tttgagcctc gctgctcaca ccatgccaga aaaggagttt
     1201 ttcctttctg atagaacatg tcatagcaat aaatgcctgg atgaatgagc gatgacggga
     1261 tcgccggaat atcaccactg atgccactgg atgttgcatt aataatgaga tcaaactcat
     1321 gaccttccag ttcgtccata cccaacgcct gaatactgcc agtgtgcgca aacaatttag
     1381 tcaactcttc tgcgcgagat atcgtccgat tagtgattgt caccgcacag tccagggaaa
     1441 ggagtggcag tagtacgccg cgagatgctc caccagcgcc aatgagcaga atacgtaaac
     1501 caggacggat aaaagacaga cgttccagat cgcttaacaa acctatacca tcggtattgt
     1561 cacccagcag gcgtccatct tctaaccgct tgagggtatt aacagcacca gccaacgctg
     1621 cccgttcagt aagctcatcc gctctggcaa aagcctcttc tttaaaaggt accgtcacat
     1681 tcgcaccttt accaccagca ctaaagaaag cgttcagtgt gttgatgaaa tcattgatgg
     1741 gtgccaacac gcgtccatag ggatgttcaa tattcagttg ctgagcaaat tgctgatgaa
     1801 tgaatggcga tttgctgtgg gctatcggat taccaaaaac agcataggtt tccattatgt
     1861 tacccctgtc gaaacagttc acccgtcagg gcatcgcgga tttctgaagg atttaaacgc
     1921 ccccccgttt caccaggcac aaccgggaac gccgcgccaa attgtgcgcg aacttcgtct
     1981 actgttcgac aaggtggcaa tccactcaag ttggcactgg tagaaaccag cggtttacca
     2041 taagcctggc acaaagcaac caccaacgga tggtcggtga ctcgtacagc aagcgaatca
     2101 aagcggcccg tcaaccagcg cggtgttgtc gcaggcgcgg gaaaaacaaa ggtgacagga
     2161 cctggccagc gagaaaatat agtttcacgc tgcgcgtcag tcagcatggt gtcatcaata
     2221 tagggtttaa gctgctcgta atttgcggcg attagaatca gccccttatc aaccggacgc
     2281 tgttttaact ccaacagtcg catcactgct gtttcgctat caggatcgca cccgacaccg
     2341 aaaacggctt ccgttggata ggcgatgaca cgttcttcat tgaggacatc tatcgcagct
     2401 gcgatagcgt ctccttgcag gttattattc acgttattat tccgccgaaa tcggctttcc
     2461 acattgttta ctggcacaaa agtgttttac accctgcgcg gttttctttt cgatgagtag
     2521 cggataatgg cactcagggc attctccagc tatgggtttg aagttaatgg caaattgaca
     2581 ctccgggtag cgatcacagg agtgaaatgt tttgccataa cgggagcggc gctgaaccag
     2641 atggcccgtc cggcattggg ggcatgtaat tgctgtttcg tccggtttat cgataagttc
     2701 ggtatgttcg cattcagggt agttactgca accaataaac ataccaaagc gtccctggcg
     2761 taataccaga tttgcgccac atgcagggca aacctgcccc tccagaactt tgacaatatg
     2821 tccatccgct gaagatttca gaggacggac gtagtcacac gccggatact gtgagcatcc
     2881 aagaaacgga ccgtgtttcc cggatcgaat aactagttca gccccgcact ttgggcagga
     2941 ctcattatta cgcaccgtga acagtgctga tttcgccata actacttatg ctgaattaaa
     3001 gaaatgatta atgcagcata ccttcattca cttcaaagag taattcttcc atttgctggt
     3061 acgcattttc gcagcccgga atattgaaca acaccatcag gattacccat ttcaggtctt
     3121 ccaggtcgaa ctcagcggta tccagcgcga gcactcgctc tatcaccatt tcacgggttt
     3181 caaggttaag cacctgaatt tgctcaagga aaagcagaaa accacgacag ctggcatcca
     3241 gtctttcaca ctcttcaggg gtataaatac gcatggagag tggatcagag gccagttgca
     3301 tcggttctgc cagtccttcc tgataatccg caagcttttc cagccaaagc agggcattgt
     3361 agatatcttc tcgatcaaat cctgcgtcgg taagatcctg ttcaagtttg tcttgatcca
     3421 cacgcaactc agcttctgtg tgaatatagg tttcaaacaa atacattagt acgtcgaaca
     3481 tggcatgccc tcctcaatcg gacatagccg ccgggtacag ctgcgatcca tcctgctaac
     3541 tccagttcga gtagttgagt aactacctct ggcacaggtt ggccggcacg ttcagcgacg
     3601 acgtcaacag gtgttacctc atctcctacg ttagccagga gctcaggaaa tggcaatgcc
     3661 acgtcttcct gatctggtga ataaaatgaa ttttcagggg cgtctggcaa ccagtgcaat
     3721 ccaaattgca agttttccag aatttcttcc ggttccgtca caagaatcgc accttgtttt
     3781 attaaccagt gaggcccttc gcttcccgga ttccctattg gacctggcaa agcaaaaact
     3841 tcacgcccct gctcaagcgc acaacgtgct gtcaccagcg aaccactgcg caaagccgct
     3901 tccaccacca gtacaccttt acttagacca ctgataatgc gatttcttcg tgggaaattg
     3961 taagcaaggg gtggaacatc gaggggaaat tccgagacga gagcaccacc ttgctcaagt
     4021 agactggcag ccagtggagc atggcggcgg ggatgaatgg tattaagtcc attccccaat
     4081 acagcaatgc taacgccatt tacctgtaag gccgctttat gcgccacacc atcgattcca
     4141 cgcgccagtc cactcgtaat tgtcactcca cgcgtcgcca gagtttcgca aaataatcgt
     4201 ccccatcgct cgccatacca tgaatgcgcc cgactcccca ctacggcaag ctgaaatgaa
     4261 tgcaacgcgt gcagttctcc ttcaacaaac agtgcgccgg ggtaatctgt cgtcgccaga
     4321 agttgaggag gataaaattc gctgtccgca gggattaaat gatggttggg ttgctccaac
     4381 caacaaagtg agctttcgat actctttcgc ggaaatgaaa gaaagcgttg tgcctgccgc
     4441 aatgtaagcc ctgtttgctg caatacaacc gcatcaatat gcgactgttt tgccagccag
     4501 tgagctatac ggaccatatc atcgccatac aagctgctga tgctaattaa acgcagccaa
     4561 atttctgtat cgaccatcct tatctccctg ccataagcag ccttagcaat ctttgcgatt
     4621 ggtcagtgat gctgtcaatc agagggggat ttgtctagaa tagaagaaat aatctttcta
     4681 actcctgaac acatctctgg agatttatgt cagttttgca agtgttacat attccggacg
     4741 agcggcttcg caaagttgct aaaccggtag aagaagtgaa tgcagaaatt cagcgtatcg
     4801 tcgatgatat gttcgagacg atgtacgcag aagaaggtat tggcctggcg gcaacccagg
     4861 ttgatatcca tcaacgtatc attgtaattg atgtttcgga aaaccgtgac gaacggctgg
     4921 tgttaatcaa tccggagctt ttagaaaaaa gcggcgaaac aggcattgaa gaaggttgcc
     4981 tgtcgatccc tgaacaacgt gctttagtgc cacgcgcaga gaaagttaaa attcgcgccc
     5041 tggaccgcga cggtaaacca tttgaactgg aagcagacgg tctgttagcc atctgtattc
     5101 agcatgagat ggatcacctg gtcggcaaac tgtttatgga ttatctgtca ccgctgaaac
     5161 aacaacgtat tcgtcagaaa gttgaaaaac tggatcgtct gaaagcccgg gcttaaggat
     5221 aagaattaac gtgtcagaat cactacgtat tatttttgcg ggtacacctg actttgcagc
     5281 gcgtcatctc gacgcgctgt tgtcttctgg tcataacatc gttggcgtgt ttacccagcc
     5341 agaccgaccg gcaggacgcg gtaaaaaact gatgcccagc ccggttaaag ttctggctga
     5401 ggacaaaggt ctgcccgttt ttcaacctgt ttccctgcgt ccacaagaaa accagcaact
     5461 ggtcgccgat ctgcaggctg atgttatggt cgtcgtcgcc tatggtttaa ttctgcctaa
     5521 agctgtgctg gagatgccgc gtcttggctg tatcaacgtt catggttcac tgctgccacg
     5581 ctggcgcggt gctgcaccaa tccaacgctc actatgggcg ggtgatgcag aaactggtgt
     5641 gaccattatg caaatggatg ttggtttaga caccggtgac atgctttata agctctcctg
     5701 cccgattact gcagaagata ccagtggtac gctgtacgac aaactggcag agcttggccc
     5761 acaagggctt attactacgt tgaaacaact ggcagacggc acggcgaaac cagaagttca
     5821 ggacgaaact cttgtcactt acgccgaaaa gttgagtaaa gaagaagcgc gtattgactg
     5881 gtcactttcg gcagcacagc ttgaacgttg cattcgcgct ttcaatccat ggccaatgag
     5941 ctggctggaa attgaagggc agccggttaa agtctggaaa gcatctgtca tcgatacgac
     6001 aacaaaggct gcgccaggaa caatccttga agccaataaa caaggcattc aggtagcgac
     6061 aggtgatggc atcctgaatc tgctctcaat gcaacctgca ggtaagaaag cgatgagcgt
     6121 gcaagacctc ctgaattctc gtcgggaatg gtttgtaccg ggcaaccgtc tggcctgata
     6181 gtccactctt ctaagcccgg tcttaccggg catctttata tttatactta tgaaaaaaca
     6241 acgtaattta cgtagcatgg cggcccaggc cattgaacaa gtcgttgagc aagggcaatc
     6301 actgagcaac attctgccac cgctccagca aaaagtttcc gataaagata aagctcttct
     6361 ccaggagtta tgctttggcg tactgcgtac gctctcacag ttagactggc tgattaataa
     6421 gctaatggcc cgtccgatga caggcaaaca gcgtactgtt cattacctga ttatggttgg
     6481 tttgtatcaa ctgctttata cccgcattcc acctcatgcc gcgctggctg aaacggttga
     6541 aggcgcagtt gcaattaagc gcccgcaact taagggattg attaacggtg tattacgtca
     6601 gttccagcgt cagcaagatg agttattagc cgagtttaat gccagtgatg cacgttatct
     6661 gcatccttcc tggttgctga agcgtctgca aaaggcgtat cccgagcagt ggcaatccat
     6721 cgtcgaagcc aataaccagc gtccgccaat gtggctgcgc gttaatcgta cgcatcattc
     6781 ccgcgacagc tggcttgcat tgctggatga agcaggaatg aaaggtttcc cgcatgcgga
     6841 ttaccctgat gctgtacagc tggaaacacc tgcacctgtt catgcgctac ccggttttga
     6901 agaaggatgg gttaccgttc aggatgcttc agcacaaggt tgcatgacct ggcttgctcc
     6961 acaaaatggt gaacacattc tggatctttg cgccgcccca ggcggtaaaa caacgcatat
     7021 tcttgaggtt gctccagagg cgcaagttct ggcggtcgat attgatgagc agcgcctctc
     7081 tcgcgtttac gacaatttaa aacgccttgg tatgaaggca accgtaaaac aaggtgatgg
     7141 ccgttaccct tcccaatggt gtggtgagca acagttcgat cgcattttat tagatgcacc
     7201 ttgttcggca actggtgtaa ttcgtcgcca tccggatatt aaatggttac gccgcgatcg
     7261 cgatatccca gaactcgcgc agttgcagtc tgaaattctc gacgccatct ggtcgcattt
     7321 aaaatccgga ggaaccctgg tctatgccac ctgttcgatg ttaccggaag agaatagcct
     7381 gcagattaaa gcctttttgc aacgtaccgc tgatgccgaa ctttgcgaaa caggaacacc
     7441 agagcaaccg ggtaaacaaa atttgcccgg tgccgaagag ggcgacggct tcttttacgc
     7501 taagctaatc aaaaagtgat gagataacgg gtcgcgactg atgaaaatta tcattctggg
     7561 tgccggccag gttggcggca cactggcgga aaacctggtt ggcgagaaca acgatattac
     7621 tgttgtcgat accaacggtg agcgtctgcg gaccttacag gataaatttg acctgcgagt
     7681 cgtgcagggg catggctctc atccacgcgt attgcgggag gcaggtgccg acgacgccga
     7741 tatgctggtt gctgtaacca gttcagatga aaccaatatg gttgcctgcc aggtagccta
     7801 ctcacttttc aacaccccta atcgcatcgc tcgtatccgc tcaccagact acgtgcgcga
     7861 tgccgataag ctatttcatt cagatgctgt tccgattgat catctgatcg caccagagca
     7921 gttggttatc gataatattt accgactgat tgagtatccc ggcgcattgc aggtggtgaa
     7981 cttcgctgag ggtaaagtca gcctggctgt ggttaaagcc tattatggcg gcccgctgat
     8041 tggtaatgca ctttcgacca tgcgcgaaca tatgccacat atcgatactc gtgtggcagc
     8101 aattttccgc cacgatcgcc ccattcgtcc gcaaggttcg accattgttg aagctggtga
     8161 tgaagtgttc tttattgccg cttcacagca tatccgcgcg gtgatgagtg aattacagcg
     8221 actggaaaaa ccgtataagc ggatcatgct ggttggtggc ggtaatatcg gtgcagggct
     8281 ggcgcgtcgt ctggaaaaag attacagcgt taaactcatc gaacgtaatc agcagcgcgc
     8341 tgccgaactg gcggaaaagt tgcagaatac gatcgtcttt tttggtgatg cgtcggatca
     8401 agaactgctg gccgaagaac atatcgatca agttgatctg tttattgccg tcaccaacga
     8461 tgacgaggcc aatatcatgt ccgccatgct tgccaaacgt atgggtgcga aaaaggtgat
     8521 ggtattgatc caacgtcgcg cttatgtgga tctggttcag gagagcgtga tcgatattgc
     8581 gatttcacca caacaagcaa ctatttctgc gttgcttagc catgtgcgaa aagcagatat
     8641 tgttggtgtt tcctcattac gccgcggcgt agcagaagct attgaagccg ttgctcacgg
     8701 tgatgaaagc acctcacgcg ttgtcggcag agtcattgac gaaatcaagc taccgccagg
     8761 aacgattatt ggagcggtgg tacgtggaaa cgatgtgatg attgccaatg acaatctgcg
     8821 cattgagcaa ggcgatcacg taattatgtt cctcacagat aaaaagttta ttaccgacgt
     8881 cgaaagactc ttccagccaa gtcctttctt cttgtaatta ataaggcgtc taatgacgcc
     8941 ttattatttc cctttgatta ttaaggatta attaaatcca ttcctggcag gaaaatggct
     9001 taacatttgt tagacttatg gttgtcggct tcatagggag aataacatga gcattattaa
     9061 agaatttcgc gaatttgcga tgcgcgggaa cgtggtggat ttggcagtgg gtgtcattat
     9121 cggagcggca ttcgggaaga ttgtatcttc actggttgcc gatatcatca tgccacctct
     9181 gggcttatta attggcggga tcgactttaa acagtttgct gtcacgctac gcgaggccca
     9241 aggggatatc cctgctgttg tgatgcatta cggtgtcttc attcaaaacg tctttgattt
     9301 tctgatagtg gcctttgcca tctttatggc gattaagcta atcaacaaac tgaatcggaa
     9361 aaaagaagaa ccagcagcag caacacctgc accaactaaa gaagaagtat tactgacaga
     9421 aattcgtgat ttgctgaaag agcagaataa ccgctcttaa caagcgtctg aaagcagaag
     9481 gccagtggta aaaaagtgat ttactttctt gccactggcc tcccagttcc cccgattgcc
     9541 atgttttcct tttcgcatgt aactgccttt ccctttctta tttttctcta ctcgctgtcg
     9601 gaataaggga tcatgtagta atgcttctat cgcattatcc tttatctgcc ctttagtatg
     9661 ctgatatcgg ctcataaaaa ctccagttgg ttatttaacg gcgcgagtgt aatcctgcca
     9721 gtgcaaaaaa tcaacagcca ctcttaacgc cactcgcccc ttgttcaaga gcttcaagaa
     9781 tcgagcaata aacactgcta tgggcggtcc cacaacaggt atcattaagg cgttgcaagg
     9841 aacgctgcat actctgcaac tcggctatac gagcttcgac ttcctgcaat cgttcctgca
     9901 caatgccttt tgactcctga caggtatggt gttccggatc gatacggatc gacagcaact
     9961 cgcggatcga ctccagactg aaacctagtt gtctggcatg gcggataaat ttcaatcgct
    10021 ggagatcgct ttcggtatac aggcgaaacc caccttcggt gcgtacttca tgctccatca
    10081 tctgctgctt ttcgtaataa cgaatcgtgt cgggtgttac ttccgccatt tttgccagct
    10141 caccaatgcg atacatacat actccactag ttatcgttga ttttgttcaa caacttgtca
    10201 gcataatcgc cgcgtaaaaa atcggtactc aatccagctt gtcgcagctt caattccagt
    10261 aatgacaatc gacgactaac ctcttgatat tgcggatcat catgacggat acctttgaga
    10321 atatccagaa gctgtattgc ttccctccta tgctcaagtt ctggcggtaa gcaaccagca
    10381 ttcttcagca agcgataccc cgcacgtaat tccggtggca catgagaatc atcatccagt
    10441 atcaatggtt cgccgctacc taccaggtta tcaaactcac ctttcgcttg cgcttctgct
    10501 atatgacgct ctgcccactg gtcaagtaac cacataaata ctccagggga tgaataaaaa
    10561 gagtacagct attgtagata agtggggatg ctacgggtat aaaaaaaccc gccggggcgg
    10621 gtttttttac gttgcttcag attactctgc agcagcttct gctttctctg aacgatcaac
    10681 cagctcgatg taagccatcg gcgcattgtc gcctgcacgg aagccacact tcagaatacg
    10741 agtgtaacca ccggcacggc tcgcgaaacg cgggcccagt tcgttaaaca gttttgccac
    10801 gatctcgtta tcacgagtac gggcgaatgc cagacgacga ttagcaacgc tatcagtctt
    10861 ggcaagagta atcagcggct caactacgcg gcgcagctct ttcgctttag gcagagtcgt
    10921 cttgatgatt tcatgacgaa ccagtgaacc tgccatattg cggaacatag cctggcgatg
    10981 gctgctgttg cggttcagtt gacgaccact cttacgatgg cgcatgacct tatccttctc
    11041 agtaaaacct taacctgtga tccggttact cgtcagcgat gcttgccggt ggccagtttt
    11101 ccaggcgcat gcccagagac agtccacggg aagccagcac gtctttaatc tcagtaagag
    11161 attttttacc aaggttaggc gttttaagga gctcaacctc ggtacgctgt accagatcac
    11221 cgatatagtg gatagcttct gctttaaggc agttagcaga gcggacagtc aattccagat
    11281 cgtcaacagg gcgcagcagg atcggatcga actctggttt ctcttctttc acttcaggct
    11341 gacgtacatc acgtaagtca acgaaagctt ccagttgttc agccagaatg gttgccgcac
    11401 gacgaatcgc ctcttcagga tcgattgtgc cgttggtttc catttcgatg accagcttgt
    11461 ccaggtcggt acgctgttct acacgcgctg cttcaacatt gtaggcaata cgctccacag
    11521 ggctgtagca tgcgtcgacc agcagacggc cgattgggcg ctcatcttct tccgaatgaa
    11581 ttcgggtaga agccggcaca taaccacgac cgcgctgaac tttgatacgc atgctaatag
    11641 acgcgttctc atcggtcagg tggcagatca cgtgctgcgg cttgacgatt tcgacatcac
    11701 cgtcgtgggt gatatcggct gcagtcacag ggccaatgcc agatttattc aaggtaagaa
    11761 taacttcatc tttgccctga actctcaccg ccagcccttt caggttgagc aggatttcca
    11821 ggatatcttc ctgaacgcct tctttggtgc tgtactcatg tagtacacca tcaatctcaa
    11881 cctcggtcac cgcgcaaccc ggcatcgatg agagcagaat acggcgcagt gcgttaccca
    11941 gagtatggcc aaagccacgc tctaaaggct caagggtcac cttggcgtgc gtcgaactca
    12001 cttgctcgat atcaaccagg cgcggtttta gaaactctgt cacagaaccc tgcattgtgt
    12061 cctctctttg gtactaagct ttacttggag taaagctcga cgatcaggtg ttcgttaatg
    12121 tccgcagaca gatcagaacg ctccggctta cgcttaaacg taccttccat cttgccagca
    12181 tcaacttcca accaggttgg cttttcacgc tgctcagcca gctccagagc ggctttcacg
    12241 cgagactgct tcttcgcttt ctcacgaatg cttacaacgt cattcggact aacctgataa
    12301 gaagcgatgt taacaacacg accgtttacc ataattgctt tatggctaac cagctgacgt
    12361 gcttctgcac gagtggcacc gaagcccata cggtatacaa cgttgtccag acgaccttcc
    12421 agcagagcca acaggttttc accggtgttg cctttcagac gtgctgcttc tttgtagtag
    12481 ttacggaact gacgctccag cacaccatag atacggcgaa ctttttgctt ttcacgcaac
    12541 tgcacaccat agtcagacag acgcggttta cgcgcaccgt gctggccagg agcttgttca
    12601 attttacact tggtatcgat cgcgcgaacg ccagacttaa ggaataagtc ggtgccctca
    12661 cgacggctca gcttgagctt aggacccaaa tatcttgcca ttttctttct ccaacaaacc
    12721 tggaaaacga ggcgttatac gcgacgtttt ttcggcggac gacaaccgtt atgagggatc
    12781 ggagtcacat cagtaatgtt agtgatgcgg aaacctgcgg cgttcagagc acgaatagta
    12841 gattcgcggc ctggacccgg acctttaacc ataacttcca gattcttgat gccgtattct
    12901 ttcacggcgt cagcgcaacg ctctgctgca acctgtgctg caaacggagt ggatttgcga
    12961 gaaccacgga aaccggaacc accggctgtt gcccaaccca acgcgttacc ctgacgatca
    13021 gtgatagtca cgatggtgtt gttgaaagaa gcatggatat gagccacgcc gtcagagact
    13081 tgttttctta cacgtttacg tgcacgaatt ggtgcctttg ccattattca atcaccccga
    13141 ttatttcttg atcggtttgc gcggaccctt acgggtacgt gcgttggtct tggtacgctg
    13201 accgcgaacc gggagaccac gacgatgacg caaaccgcga tagcaaccaa gatccatcag
    13261 gcgcttgatg ctcatgctga tttcacggcg cagatcacct tcaacgacaa atttggcaac
    13321 ttcgtcacgc agcgtgtcga tttgtccttc agacagctca ctgatcttaa catcttcagc
    13381 gatacccgct gcagccagga tggctttaga acgggtcttg ccgacgccat aaatcgaagt
    13441 taatgcgatt acggcatgct tatgatcagg aatgttaatg cctgctatac gggccactat
    13501 gcactcctac tatttaatat gtacgttcca tgctgaaaag cccgttttca ggatactcaa
    13561 atggaaacgc acagacatac aaaagattgg ctggctaatc tagccagctc aacccaactt
    13621 tgcaagaaaa atatgcgaaa aaatcagcct tggcgctgtt tatgcttcgg ctcggcactg
    13681 caaatcacac ggatgacacc atcacgctta acgattttgc agttacggca taatttcttg
    13741 acggaagcac gaactttcat ttttactctc cgtaacttct cgggcgacca attatcggcc
    13801 gtagcctttc aggttcgcct tcttcaatgc agactcatac tgactggaca tcatcagagt
    13861 ttgcacttga gccataaagt ccataatcac gacaacaacg ataagcagtg aggtcccacc
    13921 gaagtagaac ggtactttca ttgcatcacg catgaactcc gggatcaggc agataaaggt
    13981 aatgtacagc gcaccaacca aggtcaggcg ggtcattact ttatcgatat acttcgccgt
    14041 ttgctctccc ggacgaattc ctggtacaaa tgcaccggac ttcttcaggt tatctgctgt
    14101 ttcacgcggg ttgaaaacca acgccgtgta gaagaaacag aagaagatga ttgcagacgc
    14161 atagagtaac acataaagcg gttgcccagg ctgcaaatac agcgaaattg ttgtcagcca
    14221 gttccaacca gtaccgcccc cgaaccatga cgcgatggtc gccgggaaca gaataatact
    14281 ggaagcgaag attgccggga ttacccccgc catattcact ttcagcggta aatgtgtgct
    14341 ctgtgcagca tagacacgac gaccttgctg acgtttcgcg tagtttacca caatgcggcg
    14401 ttgaccacgc tcaacaaata caacaaagaa cgtcactgca aatactaata ctgcaaccaa
    14461 cagcaacacg aggaagtgca ggtcgccttg acgcgcttgc tcgatagtat gggcaatggc
    14521 tggcgggagt cccgcgacaa taccggcgaa gataatgatt gaaataccgt tgccgatacc
    14581 tcgttcagtg atctgttcgc ccaaccacat caggaacatg gttcctgtga ccagacttac
    14641 aacagcggtg aagtagaatg caaagcccgg gttaatgacc aggccttgca taccaggcat
    14701 attcggcaga ccggtagcaa taccgatcga ctggaatatt gccagcacca gagtaccgta
    14761 gcgggtgtac tggctgatct tacgacgacc agactcccct tctttcttaa tttctgccaa
    14821 cgttggatga accaccgtca gtagctggat aatgatcgac gccgaaatat acggcatgat
    14881 ccccagagca aagatagaag cacggctgag agcaccacca gagaacatgt taaacatctc
    14941 aatgatggtg cctcgctgtt gctcaagcag tttggcaagt acagcggcat caataccagg
    15001 gatcggaata aaagagccaa tacggaacac aatcagcgca ccgataacaa acagcagtct
    15061 gcgtttcagc tcgcctaagc cacctttggc actttgaaaa tctaatcccg gttgtttagc
    15121 catctgctac ttattcctcg attttaccgc cagcagcttc gatagcagca cgagcgcctt
    15181 tagtaacacg caggccacga acagttaccg gagtcgttac ttcgccagcc aggatcactt
    15241 tcgcgaactc gatctggata ccgataatgt tagccgcttt cagcgtgttc aggtctacaa
    15301 caccgccttc tactttagcc aggtcagaca gacgaacttc ggctgtaatc gctgctttac
    15361 gagaagtgaa gccgaatttc ggcagacgac ggtacagagg catctgacca ccctcgaaac
    15421 cgcgacgtac gccaccgcca gaacgagatt tctgaccttt gtgaccacga ccaccggttt
    15481 taccgaggcc agaaccgata ccacgaccca ggcgtttacc cgcctttttg gagccttcgg
    15541 ccggagacag agtatttaaa cgcatctctt actcctcaac tttaaccatg aaggaaaccg
    15601 cgttgatcat accgcgaata gcaggagtat cctcgcgctc tacggtgtga ccaatacgac
    15661 gcagacccag gccaagcagc gttgccttgt gtttcggcag acgaccgatt gcactgcggg
    15721 tttgagtaat tttaatagtc tttgccatgg tttatttccc cagaatttct tcaacggatt
    15781 taccacgctt ggcagcgacc atttctggag aattcatatt ttccaggcca tcgatagttg
    15841 cacgaaccac gttaatcggg ttggtggaac catacgcttt agccagaacg ttatgaaccc
    15901 cagcgacttc cagaacggcg cgcattgcac caccggcgat gataccggta ccttcggaag
    15961 ccggctgcat gaatacgcga gaacccgtat gaacaccttt aacagggtgt tgcagagtac
    16021 cgttattcag cgcgacgtta atcatattgc gacgggcttt ttccatcgct ttctggatcg
    16081 ctgctggaac ttcacgcgct ttaccgtaac caaaaccaac gcgaccgtta ccatcgccaa
    16141 ctacagtcag agctgtgaag gagaaaatac gaccaccttt aacggtttta gatacgcggt
    16201 ttaccgcgat cagcttttcc tgcagttcgc cagcttgttt ttcgatgtga gccatcttac
    16261 acctctacct tagaactgaa ggccagcttc acgggcagca tctgccagtg cctggacacg
    16321 accatgatat tggaacccgg aacggtcaaa ggatacatct ttgatgcctt tttccagagc
    16381 gcgttcagcg acagctttac ccacagctgc agccgcgtct ttgttaccgg tgtacttcag
    16441 ttgttcagcg atagcttttt ctacagtaga agcagctacc agaacttcag aaccgttcgg
    16501 tgcaattacc tgtgcgtaaa tgtgacgcgg ggtacgatgt accaccaggc gagttgcgcc
    16561 cagctcctgg agcttgcggc gtgcgcgggt cgcacgacgg atacgagcag atttcttatc
    16621 catagtgtta ccttacttct tcttagcctc tttggtacgc acgacttcgt cggcgtaacg
    16681 aacacccttg cctttataag gctcaggacg acggtaggcg cgcagatccg ctgcaacctg
    16741 gccgatcacc tgcttatcag cgcctttcag cacgatttca gtctgagtcg gacattcagc
    16801 agtgataccc gcaggcagct gatggtcaac aggatgagag aaacccagag acaggttaat
    16861 cacattgcct ttaaccgctg cacggtaacc tacaccaacc agctgcagct tcttagtgaa
    16921 gccttcggta acaccgataa ccattgagtt cagcagggca cgcacggtac cagcctgtgc
    16981 ccaaccgtct gcgtaaccat cacgcggacc gaaggtcagg gtattatctg catgtttaac
    17041 ttcaacagca tcgttgagag tacgagtcag ctcgccgttt ttacctttga tcgtaataac
    17101 ctgaccgttg atttttacgt caacgccggc aggaacaacg accggtgctt tagcaacacg
    17161 agacattttt tcctccgatt aggctacgta gcagataatt tcgccaccaa gaccagcctg
    17221 gcgcgctgca cgatcagtca taacaccttt agaggtagaa acaactgcga tacccagacc
    17281 cgccataact ttcggcagct catctttacg tttatagatg cgcagacctg ggcggctgac
    17341 acgctgaatg ctttctacaa cagctttgcc ctggaaatac ttcagagtaa gttccagttc
    17401 aggcttggtg tcgccttcaa ctttaaaatc ttcaataaaa ccttcttcct tcagcacgtt
    17461 ggcgattgcc actttcagct tggaggaagg catggtgacc gcagctttgt tcgcggcctg
    17521 accgttacgg atacgggtca gcatatccgc gatcggatct tgcatgctca tctgtcttta
    17581 ctcccgtgat tcaattggtg acaattacca gctagccttt ttcagacccg ggatttcacc
    17641 gcgcatagcg gcttcacgga ccttaatacg gctcaacccg aacttccgca ggaaaccatg
    17701 cggacgacct gtttgacggc agcggttacg ctgacgagac gggctggaat cacgcggcag
    17761 agtctgcagc ttgagaacag cgttccaacg atcttcgtcg gaagcgttca catcagagat
    17821 gatcgctttc agttcagcgc gtttcgcgaa gtatttatca gctaaagcta cgcgttttac
    17881 ttcgcgtgct ttcattgatt gcttagccat ttagtaaccc taccttactt gcggaacggg
    17941 aagtcaaagg cagccagcag agcgcggcct tcttcgtcag atttcgcagt agtggtaatg
    18001 gtaatatcca aaccacgaac gcggtcgact ttatcgtagt cgatttctgg gaagatgatc
    18061 tgctcacgga cacccatgct gtagttacca cgaccgtcga aagacttagc ggacaggcca
    18121 cggaagtcac ggatacgagg tacagcaata gtgatcaggc gctcaaagaa ctcccacatg
    18181 cgttcgccac gcagagttac tttacagccg atcggatagc cctgacggat tttgaagcct
    18241 gcaacagatt tgcgtgcttt ggtgatcagc ggtttttgac cggagattgc tgccaggtct
    18301 gctgctgcgt tatccagcag ttttttgtca gcgatcgctt caccaacacc catgttcagg
    18361 gtgatcttct cgacccgagg gacttgcatg acagaattgt agttaaactc agtcatgagt
    18421 tttttaacta cttcgtcttt gtagtaatca tgcagtttcg ccatcgtact actccaaatt
    18481 acttgatagt ttcgctgtta gacttgaaga aacggacttt tttaccgtct tcgaatctaa
    18541 agcctacacg gtcagccttg ccggttgtcg cattgaagat tgctacgttg gaaacctgaa
    18601 tagcggcttc tttttcaacg atgccacccg gttggttcag ggccggaacc ggcttctgat
    18661 gtttcttaac caggttgata ccttcaacaa tgaccttgcc ggaagacagg acattcttaa
    18721 ctttaccgcg tttaccttta tctttaccgg ttaacacgat aacttcgtca tcacgacgga
    18781 ttttcgctgc catgattcgc tccttagagt acttctggtg ccagagagat aattttcatg
    18841 aacttctcac tacgaagctc acgagttacc ggcccaaaaa tacgcgtacc gataggctgc
    18901 tcgctgttgt tgttcagaag aacacaagca ttaccatcga agcgaatgac agaaccgtcc
    18961 gggcgacgaa cacccttctt ggtgcgcacc actaccgcct tcagcacatc accttttttg
    19021 accttaccac gcggaattgc ttctttgatg gtgatcttga tgatgtcgcc tacgcctgcg
    19081 tagcgacggt gcgagccacc cagaaccttg atacacatta cgcgacgtgc accggagttg
    19141 tcggcgacgt tcagcatagt ctgttcttgg atcattttag tgctccgcta atgtcaacta
    19201 ctactgagac ccgaaaatca ggtcgttaaa aatccccata tcgagggcgc ggcattataa
    19261 caccgcttca aggatatggg tagaaaaaat aaacggctca tttctgagcc gtttattcgt
    19321 atcgagagag tgtactgtat tacagaaccg ctttctctac aacgcgaacc agcgtccagg
    19381 atttagtctt ggacagcgga cggcattcgc ggatttcaac cacgtcaccg ataccgcatt
    19441 cgttgttctc gtcatgtacg tgcagtttgg tcgtacgctt gatgaattta ccgtagatcg
    19501 ggtgtttcac aaaacgttcg atagcaacaa caatggattt ctccattttg tcgctaacaa
    19561 cgcgaccttg cagagtacgg attttatcgg tcattacgca cccgccttct cgttcagtaa
    19621 agtcttaacg cgtgcgacat cgcgacgcac ttgcttcaac aggtgagact gttgcagctg
    19681 gccacttgca gcctgcatac gcaggttgaa ctgctcacgc agcaggttca gcagctcggt
    19741 gttcagctct tcaacgctct tctcacgcag ctcttttgct ttcattacat caccgtctta
    19801 gttacaaagg tggttttaat cggcagtttc gctgctgcca gcttgaatgc ttcacgggcc
    19861 agctcttccg gaacaccgtc catttcatac aggactttac ccggctgaat caaggcaacc
    19921 caatactcca cgttaccttt acctttaccc atacgcactg ccagcggctt ttcagtgatc
    19981 ggtttgtccg ggaacacacg gatccagatc ttaccttgac gcttaactgc acgggtcata
    20041 gcacgacgtg ctgcttcgat ctgacgtgca gtcagacgac cacggccaac agctttcaga
    20101 ccgaagctgc cgaagctaac atccgtaccc tgcgccagac cgcggttacg gcctttgtgc
    20161 attttacgga attttgtacg ctttggttgt aacatcagcg acgctcctta tttacggcct
    20221 ttacgctgct gctttttagg ctgagcagcc ggtttttccg gttgttcaac agcagccata
    20281 ccacccagga tctcgccttt gaagatccac actttaacgc cgattacacc gtaagtggtg
    20341 tgcgcttcag aggtgttgta gtcgatgtca gcacgcagag tgtgcagcgg tacgcgacct
    20401 tcgcggtacc attcggtacg tgcgatttcc gcgccgccca gacggccgct aacttcaact
    20461 ttaatacctt tagcgcccag acgcattgcg ttctgtacag cacgcttcat agcacgacgg
    20521 aacataacgc gacgttccag ctgagaagtg atgctgtcag caaccagttt tgcgtccagt
    20581 tcaggcttac gaacttcggc gatgttgatc tgtgcaggaa cgccagcgat gtccgctacg
    20641 accttacgca gtttttctac gtcttcacct tttttaccga taacgatacc cgggcgagca
    20701 gtgtgaatgg ttacacggat gctcttagcc ggacgctcga taacgatacg agatacggac
    20761 gctttagcca gttccttagt caggtactga cgtactttaa aatcgctgtc caggttgtca
    20821 gcgaattctt tggtgttcgc aaaccaggta gagttccatg gttttacaat acccaggcga
    20881 ataccattag gatgtacttt ctgacccatt gctagtctcc agagtctcag cgatcggaca
    20941 caaccacagt gatgtggctg gtgcgcttca ggatgcgatc tgcacgacct tttgcacgcg
    21001 gcataatgcg cttcatgctc gggccttcgt ctacgaaaat tttcgtaact ttcagatcgt
    21061 caatgtcagc gccatcgttg tgttcagcgt tagcaatggc agattccaga actttcttga
    21121 ccagtacagc cgctttcttg ttggtgtagg tcaaaatatc cagagcctgc gacactttct
    21181 taccgcgaat caggtcagca acaaggcgaa ccttctgagc agaagaacga gcatggcgat
    21241 gtttagcgat agtttccatc tcttcctcct accttatttc ttcttcgctt ttttatcagc
    21301 agcgtggccg cgataagtac gagtcggtgc gaattcaccc agtttgtgac cgaccatttc
    21361 gtcggttaca aataccggaa cgtgctgacg accattatgg acagcgatgg tcaaaccgat
    21421 catgttagga aagatcgttg aacgacggga ccaagtgcgc aggggcttct tgtctccgct
    21481 ttccaccgct ttctctacct tcttcagcaa gtgcaggtca ataaaaggac ctttcttgag
    21541 agaacgtggc atggcttatc ctctaaaatt atttgctacg gcgacgtacg atgaatttat
    21601 cagtacgctt gttgctgcgg gtcttcttac ctttggtctg aacgccccac ggagttaccg
    21661 ggtgcttacc aaagttacga ccttcaccac caccatgtgg gtggtctacc gggttcatcg
    21721 cggtaccgcg aacggtcgga cgaacaccac gccagcgtgc agcacctgct ttacccagaa
    21781 cgcgcagcat atgctcagca ttgccaactt cgcccagagt tgcacggcag tctgcttcta
    21841 ctttacgcat ttcaccagaa cgcagacgca gggtgacata agcaccatca cgagcaacga
    21901 tctgaacgta agtaccagcg gaacgtgcca gctgaccgcc tttacctggt ttcatttcta
    21961 cgttatgaac agtagaacca accgggatgt tgcgcatcgg cagggtgtta cctggtttga
    22021 ttgcagcatc aacgccagac tgaatctggt cgccagcttt caggccttta ggggccagga
    22081 tgtaacggcg ttcaccgtct ttgtacagaa ccagcgcgat gttcgcggaa cggttcggat
    22141 cgtactcaag acgttcaaca actgccggga taccgtcttt gttgcgtttg aagtcaacaa
    22201 tacggtaagc ctgcttgtgg ccaccaccga tatgacgagt ggtgatacgg ccattgttgt
    22261 tacgaccacc ggatttgctg tttttttcca gcaacggagc aaaaggtttg cccttgtgca
    22321 gctcagggtt aaccacttta actacgtggc gacgacccgg agatgtcggt ttacatttaa
    22381 caactgccat tgtattactc ctccgactta ctcagcgccg ccaacgaagt ccagattctg
    22441 gccttctttc agggtgacgt aagctttttt ccagtcgcta cgacgaccga tacgctgtcc
    22501 gtgacgttta actttccctt taactaccag ggtgttaacg acttcgactt cgacttcaaa
    22561 cagtttctgc acagcagctt tgatttctgc tttggtcgcg tctttagcaa ctttgagtac
    22621 gatggtgttg gatttttcca tcgcagtaga cgctttttca gaaacgtgcg gtgcacgcag
    22681 caccttcagc agacgttctt cacgaatcat gccagcatct cctcaacttg cttaacagca
    22741 tcagcagtca ttacgacttt gtcgaaggcg atcaggctaa ccgggtcgat accagttgca
    22801 tcgcgtacgt caaccttgtg caggttgcgc gcagccagga acaggttctc gtccagctca
    22861 ccggtgatga tcagcacatc ttccagagcc atgtctttca gtttctgtgc cagcagctta
    22921 gttttcggcg cttctacaga gaacttctcg acaacgatca gacgatcctg acgtaccagt
    22981 tcggacagga tgcttttcag cgcgccgcgg tacatcttct tgttaacttt ttgactgtgg
    23041 tcctgcggac gagcagcaaa ggtcacgcca ccagaacgcc agatcgggct cttgatagaa
    23101 ccagaacgcg cacggccggt gcctttctgg cgccacggtt ttttaccgga accagttact
    23161 tcagcacgag tcttctgagc acgagtaccc tgacgagcac cagctgcata agcaacaaca
    23221 acctggtgaa ccagcgcttc gttgaaatca cgaccgaagg tagtttcgga aacagtcagc
    23281 gcgctctgcg cgtctttcaa tactaattcc attgctatct ccttacgcct tcacagctgg
    23341 tttaacgatc aggtcgctac cggttgcacc cgggacagcg cctttaacca gcagcaggtt
    23401 gcgctcagcg tcaacgcgta ctacgtcaag gctctgaacg gttacacgtt cgttacccat
    23461 ctgacctgcc attttcttgc ctttgaacac tttgcccgga gtctggttct gaccgataga
    23521 acccggaacg cggtgagaca aggagttacc gtgagtagcg tcctgggtac ggaagttcca
    23581 gcgcttaacg gtacctgcga aacctttacc tttagaggtg ccagttacgt caactttttt
    23641 aacgtcagca aacagttcaa cgctaatgct ctgacctaca gtgaactctt cgccttcagc
    23701 caggcggaat tcccacagac cacggccagc ttctacgcca gctttagcga agtgtccagc
    23761 ttcaggcttg gtcacacggt tagctttttt agcaccggtg gtcacctgaa tagcacggta
    23821 gccatcgtta gccaggtctt taacctgagt aacgcggttt gcttcaactt cgattacggt
    23881 tactgggata gaaacgccgt cttctgtgaa gatacgggtc atacccactt ttttaccgac
    23941 taaaccaatc attgtttcaa cctctcaatc gctcaatgac ctgattaacc caggctgatc
    24001 tgcacgtcta caccggcagc cagatccaga cgcatcagag catcaacggt tttctcggtt
    24061 ggctcaacga tgtcaaccag acgcaagtga gtacggattt cgtactgatc gcgcgcgtct
    24121 ttgttgacgt gcggggagat cagaacagtg aagcgctctt tgcgtgtcgg cagcgggatc
    24181 ggaccacgga cctgcgcacc agtgcgctta gcagtctcga cgatttccgc ggttgcttga
    24241 tcgatcagac gatgatcaaa cgctttcagg cggatacgga ttctttggtt ctgcatgaga
    24301 ccagagctcc aattatttta taaacgaaaa tgattactcc tcagacccat tacgattgat
    24361 gggagagtgt aaccgttctt acgtagctcc ccgattggga gcattgttag gtagccaaat
    24421 tcggctaact gaggttcaga ttgaacctgc tgtcaactac gacaagcccg cgcattatac
    24481 atacttaacc accgaacgca agcgtattgc tagtaaagta aacagccttt gggttatctc
    24541 tcaagttcgt tcagcgcatt tttgacacgc tctcttaaac cggctttctc gtcgctttct
    24601 tctacagtat tttcttcgtc ttcagaagaa gagggggcat tctcaaccgc gacgcctggt
    24661 tcatcttcct cctcgacgga ttcgacgacg gggagtggct gttttttgaa aaaatagtgt
    24721 tgtgttgatt gtttaaaagc gggcgtttca gcatctacag tgacttctgc ttttactatc
    24781 caaagtgccc atagctgccg ggcataccca ccaaccaaca ccattcctgc aaaccacagg
    24841 caaatcagca ataaacaaat gacatatatt atggtgctgt gtttttgtcg ggagaagata
    24901 ccagggtgac ccgtttcttt ctgctcacgc gcggcgatat agaattcaaa catgtcattt
    24961 ccccttcact gttgtttctg gggaaatatt tgcttcatcc cgatataagt aggcactctc
    25021 ccccgccacc tgccagagat gtactaacgt tgaaaagcca acaacaccgt cagttttcaa
    25081 gtgatggctt ttttgaaatt gctttatttt ttctaccaac aagggacgcc actctgccga
    25141 agattctgtc gaaatatgca atgcgcggtt taacatcgtt tccagccaga gaatctcttc
    25201 ctcgctgcta tcacgcgtaa tggtactctc cccttccggc gacattttcc acaacagaag
    25261 ataatcaccc gtccatacca actcaaacca tttatgtgtc agcgtccagg tttgctgccc
    25321 tacgagtaca tcaacggtag catcaccgac gcgaacgacc acaacaggca attttttgtc
    25381 cccgacttta agcgatgcga tccagggcaa attctgatcc accaatgttt gtaaagaagc
    25441 attcccactt ttacatacca gccccgctct tactgcctga tcgcaccagg ccgaatcggc
    25501 tgggacctcg tacccccata cgccgtataa cacgcttagc gcctcactat cgcgcatcac
    25561 cacatgggca atatccttag tcatatcttg tttagctact ggcgttacgg gtatcagcca
    25621 cgcaggaacg ggtaacggag ctgtgaaaga ggacagcagt agccaaccac aggtaagtgc
    25681 gataatggtt ccactcatca ccacggcaag ctgcattttc cgggggcggt tatcacctaa
    25741 tacgcggtaa atcattcgcc attgttttgc agtgaaaacg cgggtctgct cggcttgctg
    25801 taataagcta attgtttcgc cggcaagcgc cagctttctg cgatctcccc gacataataa
    25861 agctatccgt ttcagaactc tggcggttaa aaggagatcg ggctggtcaa tattctgacc
    25921 ttcaaggatg cttgccttaa gctcgtgctc cgtcagacga cctgtcgaga caagttcaat
    25981 gccctgtttt ttaaaatcac gttcaacttt tcgatcgggt ctgccgatca gcaaaaattg
    26041 accgtcagga ataagagata tcgcgcgttg gagatcatca agtatgcggc ggtgattcag
    26101 gtgagcattt tcaataataa taagagggca gcgactatca gttcgatgaa gtgcatgcag
    26161 taattgaccc gccatctcat ctcgcgtcca ggcgctgtca cggcagccct gttcagccgc
    26221 attatcgtcg gtaacgatcc atgcggcctt accttgccag gaaacaacga ggcttaaagt
    26281 aatcacacgg cgatgtttat ctttttgtag ttgctgcgcc agccaggttt ttccacttcc
    26341 agcctcaccc aacaaatagc acagacgcca ggtctgacgt ttctcacgca accaggaaag
    26401 aatgacttct cttctcgtag acatagaact tcctgtttta attattgatt atccttaatc
    26461 ataaaaataa tgtatgtatt tattaattaa atacatatta ctaatataaa taaattttgc
    26521 ttgattcatg caagcggcat taatactatt tatactaacg tcaatataca acccacccaa
    26581 tctattttat tagaacatac atcgtgccca cactaccttt ccctttccat ctggcgaatc
    26641 ataataaaga tgctgccatc aatatattga ttatatttat aagcataggg ttgattattt
    26701 ttaatgtgaa ttatttccat acaactatag ttaaaaatga acagatcatt aatcaaccaa
    26761 caaatgcatt tcagtctgaa ttcagccttg ccgctttatg gcgcaacgaa aatcatgctg
    26821 gggtaaaaga tgcaaatccc gtggcagtta atcaggaaac acctaagctc tctatagcgc
    26881 ttaatggcat tgtactcaca tcgaatgatg agacttcttt tgtcttaatt aatgagggga
    26941 acgaacaaaa gcgatactcc ctcaacgaag cccttgagtc agcgccggga acgtttatta
    27001 gaaaaataaa taaaaccagc gttgtgtttg aaactcacgg gcattacgaa aaagtaactc
    27061 tccatccagg gctacctgac atcatcaagc agcctgattc ggaaaatcaa aatgtactcg
    27121 ccgattatat tatcgccaca cctatacgtg atggagaaca gatatacggt cttcgattga
    27181 atcctcgtaa gggtctcaat gcatttacca ccagcttgct gcaacctggg gatatcgccc
    27241 tcaggatcaa taatctttcg ctgacccacc ctgatgaggt ttcacaggca ttaagtttac
    27301 tgctaactca acaaagtgct cagtttacaa ttcgtcgcaa cggcgtaccc cgcttgataa
    27361 atgtttccgt cgcggaactt acaggaatga atggacagcg caatgaaggg actcaataaa
    27421 atcacctgct gcttgctggc agcactactc atgccttgtg caggacacgc tgagaacgaa
    27481 caatacggcg ctaacttcaa taacgccgat atccgccagt tcgtggaaat agtgggtcag
    27541 catcttggca aaacgatcct gatcgaccct tcggtacagg gaaccatttc cgtacgcagt
    27601 aatgatacgt ttagccaaca ggagtactac cagttctttt taagtattct tgatctttac
    27661 ggttattccg tgatcacact ggacaatggt tttctgaaag tggtgcgctc agctaatgta
    27721 aaaacatcgc tagggatgat tgctgacagt tctcgtccag gcgtaggtga tgagttggtc
    27781 acccgaatcg taccgcttga gaacgttcct gctcgtgacc tggctcccct gctccgccag
    27841 atgatggatg cgggtagcgt cggtaatgtt gtgcattatg aaccctccaa cgttcttatt
    27901 ctgaccggtc gtgcctccac cattaataaa ctgattgaag tcataaagcg cgttgatgtc
    27961 atcggcacag agaagcagca aattattcat ctggaatatg catcagcgga agatctcgcc
    28021 gagattctta atcaattaat cagcgaaagc cacggtaaaa gccagatgcc tgccctcctt
    28081 tccgcgaaga ttgtggcgga taagcgaacc aactctctta tcatcagcgg gccggaaaaa
    28141 gcacgccagc gcataacttc attactgaaa agccttgatg tagaagagag cgaggaagga
    28201 aatacccggg tctattacct gaaatatgct aaagccacga atctggtgga agtgctaacc
    28261 ggtgtttccg aaaagctgaa agatgaaaaa gggaattcgc gtaagccttc ttctacttct
    28321 gcgatggata atgtggccat taccgccgat gaacagacca actcgttggt cattaccgct
    28381 gaccagtccg tccaggagaa actcgccacg gtaattgcgc gtctggatat tcgccgtgca
    28441 caggtgctgg ttgaggcaat catcgtcgaa gttcaggatg gaaatggact caacctcggc
    28501 gtgcaatggg cgaataaaaa cgttggcgca cagcaattta ccaatacggg attaccggtt
    28561 tttaacgctg cgcaaggtgt agctgattat aaaaagaatg gcgggatcac cagcgcgaat
    28621 cctgcctggg atatgtttag cgcctacaat ggcatggctg caggcttctt caatggcgac
    28681 tggggcgtat tgttgactgc gctggccagt aacaataaaa atgacatcct cgccaccccg
    28741 agcatcgtaa cgctggataa taaactcgcg tccttcaacg ttggtcagga tgtgccggtg
    28801 ctatccggct ctcagaccac ttctggggat aacgtcttta atactgtcga acgcaaaacg
    28861 gtgggcacaa aactcaaagt cactccgcag gtcaatgaag gcgacgcggt actgctcgaa
    28921 atagagcagg aggtttccag cgttgactct tcgtctaatt cgacgctcgg cccgacattt
    28981 aacacccgta ctattcaaaa cgctgtgctg gttaaaaccg gtgaaacggt ggtcctgggc
    29041 ggattgctgg atgatttttc taaagagcaa gtgtcaaagg ttcctctgct tggcgatatt
    29101 cctttagtgg gacaactctt ccgctatacc tcgaccgagc gcgctaaacg caacctgatg
    29161 gtatttatcc gcccgacgat tatccgtgac gatgatgttt atcgctcact gtcaaaagag
    29221 aaatacaccc gctaccgtca ggagcaacag ctgcgaatcg acgggaaatc aaaagcgttg
    29281 gttggctcgg aagatttgcc ggtgctgaat gagaacacgt tcaacagtca cgcccctgcg
    29341 ccatcgtcac ggtgaggcat tcttatgaga attcactcac cgtaccccgc cagttgggcg
    29401 ctggcacaac gaattggtta tctctattca gagggcgaga ttatttatct cgccgatacg
    29461 ccattcgaac ggttactcga tattcaacgt caagttggcc agtgccagac catgaccagc
    29521 ttgtcacagg ctgattttga agcccgactg gaagcggtgt tccatcagaa taccggtgag
    29581 tcgcaacaga ttgcgcagga tatcgatcaa tccgtcgatc ttctctcgct ttcggaagag
    29641 atgcccgcaa atgaagatct cctgaatgaa gattcagcgg caccggttat ccgcttgatc
    29701 aatgctattt tgagtgaggc catcaaagaa accgcctccg atatccacat tgaaacctat
    29761 gaaaaaacaa tgtcgatccg ttttcgcatc gacggcgttt tgcggacaat tttacagccc
    29821 aacaaaaaac tggcggcact gcttatctcc cgaattaagg tcatggctcg tcttgatatc
    29881 gccgaaaaac gtattccaca ggatggaaga atcagtttgc gtatcgggcg acgtaacata
    29941 gatgtccgcg tatccacact gccatccatc tatggtgaac gcgctgtgct ccgcctgctg
    30001 gataaaaaca gcctccagct ttcattgaac aacctgggga tgacggcagc ggataaacag
    30061 gatttagaaa atctcattca gcttccgcac ggtattatcc tggtaacagg gccgacaggc
    30121 tccggtaaaa gcaccacgct ctacgccatt ctttcggcgc ttaatacccc tggccgtaat
    30181 attctgacgg tagaagatcc cgtggaatat gaactggaag gcataggaca aacgcaggtg
    30241 aatacccgcg tggatatgtc tttcgctcgc ggcctgcgcg ccatacttcg ccaggacccg
    30301 gatgtcgtca tggtggggga aattcgtgat acagaaaccg cgcaaattgc ggttcaggcc
    30361 tcgctcacag gccatctggt actctcaaca ctccacacta acagtgcatc aggcgcagtc
    30421 acccggctac gcgacatggg cgtcgaatca ttcctgcttt cgtcttcact ggcagggatt
    30481 atcgcgcaac gtctggttcg tcgtctgtgt ccgcaatgcc gacaattcac gcccgtatcg
    30541 ccgcaacaag cgcagatgtt taaatatcat cagctcgcgg tgacaacaat tggcactccc
    30601 gtaggttgcc ctcattgcca tcaatccggt tatcaggggc gcatggcgat ccacgaaatg
    30661 atggtggtga cgccggaatt gcgggccgct attcatgaaa atgtggatga acaagcactg
    30721 gagcgactgg tccggcaaca acacaatgcc ttaatcaaaa atggcctgca aaaagtgata
    30781 cgcggtgaca cctcctggga tgaggttatg cgcgtcgcca gtgccacgct ggagaacgaa
    30841 gcatgaatta tcgctatcgc gccatgaccc aggatggtca aaaattgcag gggatcattg
    30901 atgctaacga tgaacgtcag gcacgactgc ggctgcgtga agaagggctt ttcctgttgg
    30961 atattcgccc ccaaaaaagt tcgggagtaa aaacacgtcg cccgaggatc agccatagtg
    31021 aactaacgct tttcacccgg cagttggcaa ccttaagcgc agcggcatta cccctggaag
    31081 agagccttgc cgtaattggt caacaaagca gtaataatcg actggctgac gtgttaaatc
    31141 aggtacgcag cgccatcctt gaagggcatc ccctttccga tgcgttacaa cattttccca
    31201 cgcttttcga ttcgctctac cgtaccctgg taaaagcggg cgaaaagagc ggtctgctgg
    31261 ccccggtgtt ggaaaagctg gctgattaca atgaaaaccg gcagaaaatc cgcagcaagc
    31321 tcattcagtc actgatctac ccctgcatgc tcactacggt ggcgattgtg gtcgtgatta
    31381 ttctcctcac tgctgtcgtg cccaaaatta ccgaacagtt cgtgcatatg aagcagcaac
    31441 tgccactgag tacacgcatt cttttaggtc tgagcgacac gttgcaacgt accggcccga
    31501 cattattagc gacagtattt attgtcgctg tgggtttctg gctctggtta aaacgcggca
    31561 ataaccgcca ccgttttcat gccatgttac tgcgcgttgc gctcatcggc ccgctgattt
    31621 gcgccattaa cagcgcacgc tatctccgca ctttaagtat tttgcaatcc agcggcgtcc
    31681 ctctgctgga tgggatgaat ttgtccaccg aaagcctcaa caacctcgaa attcgccagc
    31741 gcctggcaaa tgcggcagaa aacgttcgcc agggtaacag cattcatctt tcactggaac
    31801 aaaccgcaat tttcccgccg atgatgctct acatggtggc ctctggcgaa aaaagcgggc
    31861 aactcggcac gttaatggtc agagccgcag ataaccagga gacactccaa caaaatcgga
    31921 tcgccttaac gctctccatc ttcgagccag cactcattat tacgatggca ctgatcgtcc
    31981 tgtttattgt cgtgtcggta ctccaacctc ttcttcaact taactcaatg attaattaag
    32041 gaaatcatat gcgcgcaacg gataagcaac gcggttttac attactggaa attatggtgg
    32101 taattgtcat cattggcgta ctcgccagcc tggtggttcc caacctgatg ggcaataaag
    32161 aaaaggcgga taaacaaaaa gccgtcagcg atattgtggc gctggaaaac gccctggata
    32221 tgtacaagct cgacaatcat cgctatccaa ccacaaatca ggggcttgaa tccttagtag
    32281 aagcgccgac actgccaccg ctggccgcaa actataacaa ggaaggttat atcaagcgtc
    32341 tgcctgccga tccctggggc aatgattatg tcctcgttaa tcctggtgag catggtgcat
    32401 acgatctgct ttcagcagga cccgatggtg aaatgggaac cgaggacgac atcaccaact
    32461 ggggtttgag caagaagaaa aagtaagtca gcgatgaatc agcaacgcgg gtttaccctg
    32521 ctggagatga tgctggtgct ggcgctggtc gctatcacgg caagtgtggt gctcttcaca
    32581 tatggtcggg aggatgccgc cagcactcgt gcgcgcgaaa cagcggcgcg gtttaccgct
    32641 gcacttgaat tagccatcga ccgcgccacg ctcagtggtc agcctgtcgg tatccatttc
    32701 tctgattcag catggcgcat tatggtaccg ggcaaaacac catcagcctg gcgctgggtt
    32761 ccattacaag aagatgccgc agacgagagc aaaaatgact ggggtgaaga actctcaatt
    32821 caacttcagc catttaaacc tgatgacagc aaccaaccgc aggtggtgat tgtggccgac
    32881 ggacaaatca cgcccttctc tttgttgatg gctaatgccg ggaccggtaa gccgctcctg
    32941 acgttagtca gttcaggctc atggcccctg gatcaaacgc tcgccagaga tacccgccca
    33001 tgaacaaaca atcagggatg acactgcttg aagtcttact ggcgatgagt attttcaccg
    33061 ctgttgcgct gactttaatg agcagtatgc aagggcaacg aactgccatc gagcggatgc
    33121 gtaatgagac gctggcattg tggatagcgg ataaccagct tcagtcacaa gattcattcg
    33181 acgaggaaaa cacgtccagt tctggcaaag agttaataaa tggcgaagag tggaactggc
    33241 gcagcgatat ccactcaagc aaagacggca cgcttctgga acacaccata acggtgacat
    33301 tacccagcgg tcagacaacc tcgcttacgc gctatcaaag catcaataat aaatccgggc
    33361 aggcacaaga tgattaatcg ccagcagggt ttcacattgc tggaagtcat ggcggcgctg
    33421 gcgattttct caatgcttag cgtgctggcg tttatgattt tttctcaagc ttccgagttg
    33481 catcaacgtt cacagaaaga aatccagcag ttcaatcagc tacagcgcac catcaccatt
    33541 cttgataacg atctcctgca actggtcgcc cggcggaatc ggagtacgga caaaatcatg
    33601 gtgttgggtg aagaagcgat ttttaccacg caaagtcgcg atccgctggc ccctctcagt
    33661 gaagcacaaa ccttactcac tgttcactgg tatctgcgaa atcatacgct ctaccgtgct
    33721 gttcgtactt ctgtggatgg caggaaggat caacccgccc aggcgatgct ggaacatgtc
    33781 gagagctttc ttctggaaag taacagcgga gaaagccagg agcttcctct ctcagtcacg
    33841 ttacatctga agactcagca atatggggcg ttacagcgcc gttttgcatt acctgagcag
    33901 ttagcgagag aagagtcgcc agctcagacg caggccggga ataataacca tgaataatga
    33961 acaacgcggc gtagcactgc tcattgtact catgttactg gcgctcatgg ctgctctggc
    34021 tgcagacatg acgatcagtt ttcacggcca gcttcatcga acccgccagg taaaccatca
    34081 tttgcaacgg caatatgata ttgagctggc ggagaaactt gcgcttgcca gcctcaccca
    34141 ggacgttaaa gataacgaca gacaaaccac gttgcagcag tactgggcac aaccgcaaca
    34201 gttgcagctg gaaaatggca atacagtgaa gtggcagtta cgcgatgccc agcactgttt
    34261 taatctcaat gccttagcaa aaatctctga cgccccgctg gcttcccctg atttccccgt
    34321 ccaggtattc agtgccctgc tgattaacgc cggtatcgat cgcggaaaca ccgatgagat
    34381 cgtgcaatca atcgctgact atattgatgc cgatgattcg ccacgctttc acggcgcaga
    34441 agataacttt taccaaagcc aaacgccgcc tcgccatagc gccaatcaaa tgctttttct
    34501 gaccggtgaa ttgcgtcaga taaaaggcat aaccgaaaac atctatcagc ggcttatccc
    34561 gtatgtctgc gttctcccga cctcggaact tagcatcaat cttaatatgc taacggagaa
    34621 cgatattccg ctcttcagag cattattcct caataacatc acggatgctg acgctcgggt
    34681 cttattacaa aaaaggccca gggaaggctg gctcaccaca gatgcatttc tttactgggc
    34741 gcagcaggat ttctccggcg tgaagcccct ggttgctcag gtgaaagggc atctctttcc
    34801 ctatagccgc tattttacgc tgaatacgga aagtatcagc gacgaacaat cccagggatg
    34861 gcaaagccat atttttttca accgtaaaca gcagagcgcc caaatctatc ggcgtacgct
    34921 gcaactctac taatcataag gatcgtgatg cctgaatcgt tgatggtaat acgttctttc
    34981 tcgacgctgc gtaagcattg ggaatggatg acgttctccg cagatagcgt atcttcggta
    35041 catacgctca cagacgatct tccgctggag tcgctggccg atcagcccgg tgccggaaat
    35101 gttcaccttt tgatcccacc tgagggtctt ttatatcgtt ccctcacgct gcccaacgcc
    35161 aaatacaaac tcaccgcgca aacattacag tggttggcgg aggagacatt acccgacgcc
    35221 agccaaaatt ggcactggac ggttgtcgat aaacaaaacg aaagtgtcga ggtgataggc
    35281 attcagtcgg agaaatttag tcgttatctt gagcgtttac atactgccgg actcaacgtg
    35341 acgcgggttt tgccagatgg ctgctatctt ccctgggaag tagatagctg gactctggtt
    35401 aaccagcaaa caagctggct tattcgcagt gccgcacatg ccttcaacga actggatgaa
    35461 cactggttac agcatcttgc taaccagttt ccgccagaga atatgcgttg ttatggtgta
    35521 gcgcctcacg gcgttgcagc ggccaatcca cttatccagc atcctgaaat accatcactg
    35581 agtttgtatt ctgccgatat tgcattccag cgctatgaca tgctgcatgg tgtttttcgt
    35641 aaacagaaaa ctgtaagcaa atcgggaaaa tggctcgccc gcctggcagt aagctgtctc
    35701 gttctctcca ttctttcttt tgttggcagc cggggcatcg cgttctggca aacgcttaag
    35761 attgaagatc aacttcagca acaacagcaa gaaacctggc aacgttattt cccgcagatc
    35821 aaacacaccc acaactttcg tttttacttt aagcagcaac tcgctcagca atatcctgaa
    35881 gctgtaccgt tgctctatca tttacaaaca cttctgcttg aacaccatga actgcagtta
    35941 atggaggcga actatagcca gaggcaaaag tcgctgacct taaaaatgag tgctaagagt
    36001 gaagccaata tcgatcgttt ctgtgagtta acacagtcct ggcttccgat ggaaaaaaca
    36061 gaaaaagatc cggtcagtgg tgtatggaca gtaaggaact cagggaaatg attaaatcat
    36121 ggtgggcaga aaaatccaca tcggaaaaac agatcgttgc ggcattggcg gttctctcgc
    36181 tcggcgtttt ttgttggctt gcggtgataa aaccgataga tacctacatt gaagagcatc
    36241 agtcccacgc gcaaaaaatt aagaaggaca tcaaatggat gcaggatcaa gcgagcaccc
    36301 atggtttact cggtcatcca gcgcttacgc agcccatcaa aaatatcctt ctcgaagaag
    36361 caaaacgtga gaatttgact atcacgctgg aaaacggtcc ggacaatact ctgacaatta
    36421 accctgtcac agctcccctc gaaaacgtct cccgctggct cactacggca cagataacat
    36481 acggtattgt tattgaagac cttcagttta ctcttgccgg aaacgaagag atcaccctca
    36541 agcatttgtc cttcagggag caacaataat gacaatgcta ctgccgctct tcattctggt
    36601 tggttttatt gcaggttatt ttgttaatgt catggcctat cacctctcgc ctttggagga
    36661 taaaacggcg ttaacgtttc gccaggtatt ggttcatttc tggcaaaaaa aatatgcctg
    36721 gcatgataca gtgcccctga tactttgcgt tgctgctgct atcgcctgcg ccctggcacc
    36781 tttcacgccc attgtgactg gcgcactctt tctctatttc tgtttcgcac tcacgctcag
    36841 tgttattgat tttcgcactc agctcctgcc cgacaaactc accttaccgc tgctctggct
    36901 tggcttggta tttaatgcgc agtctggatt aattgattta catgatgcgg tttatggcgc
    36961 ggtcgctggc tatggggtgc tgtggtgtgt ttactggggc gtctggttag tttgtcacaa
    37021 agagggattg ggctacggtg atttcaagct actggccgct gcaggcgcgt ggtgtggctg
    37081 gcaaacgttg ccaatgatac tgctgattgc ctcgctgggt ggcattggtt acgccatcgt
    37141 ttcacaactt ctgcaacgcc ggactataac cactatcgca ttcggcccgt ggcttgcgct
    37201 cggcagcatg ataaacctgg gatatctggc ctggatctct tattaaccgg gagggttctc
    37261 cctcccgaca cggctcaacc ttcttcgcga atctgtgctt gcagataatt ttgcagtccc
    37321 atcttctgaa tcagatcaag ttccgtttcc agccagtcga tatggccttc ttcatcgcgc
    37381 aaaatttcta tcatcatatc gcggctgacg taatcatgaa cgctatcggc ataaccaatt
    37441 gcctcacgca aattcttcgc gccatccagc tcaagtgcca gatcagaacg cagcatttcc
    37501 tcaacatctt caccaatgtt cagtttgccc aggtcctgta agtttggaag accttccaga
    37561 aaaagaatgc gctcaatata acgatcggcg tgtttcatct catcaatgga ttcatgatac
    37621 tccacatcat tgagacgttt gagaccccag tttttaaaca tccgggcatg aagaaagtac
    37681 tgattgattg cgacaagctc atttcccaac agtttgttga gataatttat aactttagta
    37741 tcacctttca ttttttgact ccttcgcttc cactctttga agcgtagaac ggcttacgag
    37801 taagtcaaaa aacacactcc gtttatgcgg actccttaaa ctccggcagt tgcattaatt
    37861 catcctccat cacttcacgc gcggcccgga cacatttacc gcactgattg ccgaccggaa
    37921 tgaatttttt taattgctgg aacgagtggg gggaaaactg gcgcacagcc tgacgaattt
    37981 ttttatcgct gataccatta caaagacaaa cgtacataga gactcccgta tactttcttc
    38041 aaaagtaaat gaaaatagtt cttatttcaa ttaca
//
LOCUS       DAZFRE010000040        38032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFRE010000040 DAZFRE010000000
VERSION     DAZFRE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.040"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(102..389)
                     /gene="yebO"
                     /locus_tag="ACHKUI_002369"
     CDS             complement(102..389)
                     /gene="yebO"
                     /locus_tag="ACHKUI_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6598555.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(464..607)
                     /gene="mgrB"
                     /locus_tag="ACHKUI_002370"
     CDS             complement(464..607)
                     /gene="mgrB"
                     /locus_tag="ACHKUI_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6598556.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            766..1005
                     /gene="yobH"
                     /locus_tag="ACHKUI_002371"
     CDS             766..1005
                     /gene="yobH"
                     /locus_tag="ACHKUI_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6598557.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1149..1940)
                     /gene="kdgR"
                     /locus_tag="ACHKUI_002372"
     CDS             complement(1149..1940)
                     /gene="kdgR"
                     /locus_tag="ACHKUI_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6598558.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2117..3490
                     /gene="yebQ"
                     /locus_tag="ACHKUI_002373"
     CDS             2117..3490
                     /gene="yebQ"
                     /locus_tag="ACHKUI_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6598559.1"
                     /translation="MPKVQADGLPLPQRYCAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3536..4417)
                     /gene="htpX"
                     /locus_tag="ACHKUI_002374"
     CDS             complement(3536..4417)
                     /gene="htpX"
                     /locus_tag="ACHKUI_002374"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6598560.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4609..6657)
                     /gene="prc"
                     /locus_tag="ACHKUI_002375"
     CDS             complement(4609..6657)
                     /gene="prc"
                     /locus_tag="ACHKUI_002375"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6598561.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6677..7375)
                     /gene="proQ"
                     /locus_tag="ACHKUI_002376"
     CDS             complement(6677..7375)
                     /gene="proQ"
                     /locus_tag="ACHKUI_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6598562.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7472..7969)
                     /gene="msrC"
                     /locus_tag="ACHKUI_002377"
     CDS             complement(7472..7969)
                     /gene="msrC"
                     /locus_tag="ACHKUI_002377"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6598563.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8099..9382
                     /gene="yebS"
                     /locus_tag="ACHKUI_002378"
     CDS             8099..9382
                     /gene="yebS"
                     /locus_tag="ACHKUI_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6598564.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9351..11984
                     /gene="yebT"
                     /locus_tag="ACHKUI_002379"
     CDS             9351..11984
                     /gene="yebT"
                     /locus_tag="ACHKUI_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6598565.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12064..13503
                     /gene="rsmF"
                     /locus_tag="ACHKUI_002380"
     CDS             12064..13503
                     /gene="rsmF"
                     /locus_tag="ACHKUI_002380"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6598566.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13622..13858
                     /gene="yebV"
                     /locus_tag="ACHKUI_002381"
     CDS             13622..13858
                     /gene="yebV"
                     /locus_tag="ACHKUI_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6598567.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13963..14154
                     /gene="yebW"
                     /locus_tag="ACHKUI_002382"
     CDS             13963..14154
                     /gene="yebW"
                     /locus_tag="ACHKUI_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6598568.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDIYNTEQQCLYSMSDQLIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14155..14811)
                     /gene="pphA"
                     /locus_tag="ACHKUI_002383"
     CDS             complement(14155..14811)
                     /gene="pphA"
                     /locus_tag="ACHKUI_002383"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6598569.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNHQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     ILWSRSRLGEHQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15206..15547)
                     /gene="yebY"
                     /locus_tag="ACHKUI_002384"
     CDS             complement(15206..15547)
                     /gene="yebY"
                     /locus_tag="ACHKUI_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6598570.1"
                     /translation="MMRKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15560..16432)
                     /gene="copD"
                     /locus_tag="ACHKUI_002385"
     CDS             complement(15560..16432)
                     /gene="copD"
                     /locus_tag="ACHKUI_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6598571.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16436..16810)
                     /gene="yobA"
                     /locus_tag="ACHKUI_002386"
     CDS             complement(16436..16810)
                     /gene="yobA"
                     /locus_tag="ACHKUI_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6598572.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16949..17179
                     /gene="holE"
                     /locus_tag="ACHKUI_002387"
     CDS             16949..17179
                     /gene="holE"
                     /locus_tag="ACHKUI_002387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6598573.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17281..17937
                     /gene="yobB"
                     /locus_tag="ACHKUI_002388"
     CDS             17281..17937
                     /gene="yobB"
                     /locus_tag="ACHKUI_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6598574.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17961..18623
                     /gene="exoX"
                     /locus_tag="ACHKUI_002389"
     CDS             17961..18623
                     /gene="exoX"
                     /locus_tag="ACHKUI_002389"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6598575.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18620..20680)
                     /gene="ptrB"
                     /locus_tag="ACHKUI_002390"
     CDS             complement(18620..20680)
                     /gene="ptrB"
                     /locus_tag="ACHKUI_002390"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6598576.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVRRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20889..21548)
                     /gene="yebE"
                     /locus_tag="ACHKUI_002391"
     CDS             complement(20889..21548)
                     /gene="yebE"
                     /locus_tag="ACHKUI_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6598577.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21875..22231)
                     /gene="yebF"
                     /locus_tag="ACHKUI_002392"
     CDS             complement(21875..22231)
                     /gene="yebF"
                     /locus_tag="ACHKUI_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6598578.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22298..22588)
                     /gene="yebG"
                     /locus_tag="ACHKUI_002393"
     CDS             complement(22298..22588)
                     /gene="yebG"
                     /locus_tag="ACHKUI_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6598579.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22722..23900
                     /gene="purT"
                     /locus_tag="ACHKUI_002394"
     CDS             22722..23900
                     /gene="purT"
                     /locus_tag="ACHKUI_002394"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6598580.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23956..24597)
                     /gene="kdgA"
                     /locus_tag="ACHKUI_002395"
                     /gene_synonym="eda"
     CDS             complement(23956..24597)
                     /gene="kdgA"
                     /locus_tag="ACHKUI_002395"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6598581.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24634..26445)
                     /gene="edd"
                     /locus_tag="ACHKUI_002396"
     CDS             complement(24634..26445)
                     /gene="edd"
                     /locus_tag="ACHKUI_002396"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6598582.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLTRIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26680..28155)
                     /gene="zwf"
                     /locus_tag="ACHKUI_002397"
     CDS             complement(26680..28155)
                     /gene="zwf"
                     /locus_tag="ACHKUI_002397"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6598583.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28493..29362
                     /gene="hexR"
                     /locus_tag="ACHKUI_002398"
                     /gene_synonym="yebK"
     CDS             28493..29362
                     /gene="hexR"
                     /locus_tag="ACHKUI_002398"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6598584.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29490..30932
                     /gene="pyk"
                     /locus_tag="ACHKUI_002399"
     CDS             29490..30932
                     /gene="pyk"
                     /locus_tag="ACHKUI_002399"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6598585.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31064..32035)
                     /gene="lpxM"
                     /locus_tag="ACHKUI_002400"
                     /gene_synonym="msbB"
     CDS             complement(31064..32035)
                     /gene="lpxM"
                     /locus_tag="ACHKUI_002400"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6598586.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32154..33476)
                     /gene="mepM"
                     /locus_tag="ACHKUI_002401"
     CDS             complement(32154..33476)
                     /gene="mepM"
                     /locus_tag="ACHKUI_002401"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6598587.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33492..34424)
                     /gene="znuA"
                     /locus_tag="ACHKUI_002402"
     CDS             complement(33492..34424)
                     /gene="znuA"
                     /locus_tag="ACHKUI_002402"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6598588.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34503..35258
                     /gene="znuC"
                     /locus_tag="ACHKUI_002403"
     CDS             34503..35258
                     /gene="znuC"
                     /locus_tag="ACHKUI_002403"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6598589.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35255..36040
                     /gene="znuB"
                     /locus_tag="ACHKUI_002404"
     CDS             35255..36040
                     /gene="znuB"
                     /locus_tag="ACHKUI_002404"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6598590.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHKUI_002405"
     CDS             complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHKUI_002405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6598591.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHKUI_002406"
     CDS             complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHKUI_002406"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6598592.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37954..38019)
                     /gene="yobI"
                     /locus_tag="ACHKUI_002407"
     CDS             complement(37954..38019)
                     /gene="yobI"
                     /locus_tag="ACHKUI_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO6598593.1"
                     /translation="MYIFITHFFAEYVIFKYLLPI"
BASE COUNT         9139 a         9790 c         9346 g         9757 t
ORIGIN      
        1 accagagttt taatttgaag cagctgcacc atgacagtgc gtataatcag catgaattga
       61 gtaacccttc aacgacgggg ttactcaaga ttgaggaagg attatcgctc ggcgaccaga
      121 cgaataatat cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc
      181 tcaggttcgt ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc
      241 agcaacgcct caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa
      301 aaccacaaga tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc
      361 atcacgcctg aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta
      421 ccactgctgg agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact
      481 ggttaatggc acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata
      541 cctgcgccca aagcagcaag caagccaaca ccacgacaac cagagcgacc catcgaaact
      601 ttttcactcc aatctccgtt tcacctacct tatgtcatta aactagcatg cgttagttaa
      661 acagcgccta actatgtcga tattcgtgct ttttcggaat gagtcgcttg cttcagcgca
      721 tattgccgct acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt
      781 cgcacagtta tgctgatagc gctggtatgg ataggattat tactcagcgg atatggcgtt
      841 ttgattggaa gcaaagagaa tgccgcggga ttaggtttac aatgtaccta tctgaccgcc
      901 agaggcacca gcacggtaca atacctgcac actaaaagtg gttttctggg gataacagat
      961 tgcccgttgt tacgtaaaag caatattgtg gtcgataatg gttaagggta ataaataacc
     1021 agattctcac tcatgagtaa aatattctta tcgcaagtgc gtttaatata acgccacctt
     1081 caactttatt tttctcatgc taaataaaga acctgtctgt ttgatattca gacaggttct
     1141 tactttcctc agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgccgcg
     1201 gtatgcaaca ttgcgacata ttcctgtaaa cgctcttcag agaaacgcaa cgtcgggaag
     1261 gagatgctca gaccggcaat gaccacgcca aagcgatcaa ataccggaac cgcaatgcaa
     1321 cgcagtcctt cttcctgctc ttcattatct tcgccatacc cctgctcgcg cacctggtcc
     1381 agaacgggta ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc
     1441 acgccctcaa gaatttgcat cacttcatcg cgatcgcgcc atgccagcag taccttacca
     1501 atcgcggtgc tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac
     1561 attgagtcaa ttttgtggat gtaaacaata ctatcttcgt ccagtgcgcc gaggtggata
     1621 gtttctttgg tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg
     1681 acgttttgca acgcgcgagc gcccagttca aacaatttca gggtcagcga atatttctcc
     1741 gactcccctt cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg
     1801 gtgctttttg acatcatgac gcgctgcgac aactcggtta tccctatttc gcgctcttca
     1861 cccagcgcct gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta
     1921 tccagatctg cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg
     1981 ttttttatta taatttcgca ccagggtggt cgcaatccat cttttgccgg ttagttacaa
     2041 ttctgcgaca tccaccgtga atatcagtgc tagaatcata cccctgttga ttattcacca
     2101 aagatataaa attcctatgc caaaagttca ggccgacggc ctgccattgc cccagcgata
     2161 ctgtgcgata ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc
     2221 caacgtcgcc ttgccaacaa tcgctacgga ccttcatgcc acgccggcca gttccatctg
     2281 ggtagtgaac gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg
     2341 cgatatgttt ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc
     2401 actgttctgc gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg
     2461 tttcggcggt gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca
     2521 acgttttctg ggtagaggga tgggcataaa ctcgtttata gttgccgtct cttctgctgc
     2581 cgggccgaca attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat
     2641 caacgtaccg ttgggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg
     2701 ttctcgcgcc agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt
     2761 cggcctgctt attactgcat tgagtggttt cgctcagggg caatcactga cattgattgg
     2821 tgcggaactg gtggtaatgg ttgtcgttgg tattttcttt attcgccgcc agctttctct
     2881 tcccgtaccg ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg
     2941 cacatctgtt tgctctttct gcgcacaaat gctggcaatg gtttcccttc ccttttacct
     3001 gcaaaccgtg ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt
     3061 agcaacgatg gtgatggctc cactggcagg ctatttgatt gaacgcgtac atgcaggatt
     3121 gctgggtgct ttagggttat tcatcatggc tgcggggctt ttttccctgg ttctgctgcc
     3181 cgcgtcacct gcggatatca atattatctg gccgatgatc ttatgtggcg ctggatttgg
     3241 cttgttccag tcacccaata accacaccat tattacctcc gctcctcgcg aacgtagcgg
     3301 tggagccagt ggcatgttag ggacggctcg tcttctgggt cagagtagcg gcgcggctct
     3361 ggtagcgctg atgctaaatc agtttggtga taatggtacg cacgtctcgc tgatagctgc
     3421 ggctattctg gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgatc
     3481 cagggcataa aaaaagcgcg tcgatcagga cgcgcttttt agtatttact tcatattact
     3541 tcaggtattc acccgtacgc agagcttcaa tacgtttatc cagcggcggg tgagtcatga
     3601 acaactcact gagcgatttc gacttcccgt taatgcagaa agccatcatg ctggttgctt
     3661 cttgcggttc atagctggtt ttcaggcgtt gcagcgcggc aatcattttc tcgcgaccaa
     3721 ccagttttgc tgaacccgca tcggcgtgga attcacgatg acgcgagaac cacatggtta
     3781 taatgctcgc cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca
     3841 gcgggttgcc gttgctctct tcaccttcat cacggtttcc gcccataaaa cccgcggcaa
     3901 gctgcgccag aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg
     3961 tcatggtgac catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg
     4021 cttcatccgg gctcatgttc tgcagcaaac cggtgctgac agcaaccaga gaggcatcac
     4081 ggcgcgcacc ggttgcaaaa gcattgatgt ccggcgcatg gtagatagcc acttgcggca
     4141 tagcgatccc cgcctgacga gcctgggttg ctacagtatt gaccagccaa cgttcccttt
     4201 cgttacgcgg ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca
     4261 tcagaagcga aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct
     4321 gaacgctgct cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg
     4381 ccaggttcgt tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta
     4441 acaaaacgca atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga
     4501 tagtgcgtaa gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat
     4561 cttgccgctg ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt acttgacggg
     4621 agcgggttgt tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac
     4681 cgtctcatcc agataaggat ctggctcctg gtaatctttc ggtagatcat ccagtttctt
     4741 caactccggt ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc
     4801 atcttcatta ttctctttct cacgcacagc gtaattcaga gaaacgatat tgcgcttgtc
     4861 cttcatagcg ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc
     4921 gatacgcgca ttatgttcct tcagcagctc cggttcaaag gccgttaaat ctcctgattt
     4981 cacataagtc gcggcatcaa tgctatccca cggcagcgcg ttatcttcga atttctcacc
     5041 cgtttccgtt tcttcattac ccgtcggcat gatgatgtct ggcgttacgc ctttacgttg
     5101 cgtactgccg ccgttaacgc gatagaattt ctggatcgtg tactgcacag aacccagcgc
     5161 tggccattca ggacgtaaca tctgatcgta aatacggttc aatgaacggt actgctgaac
     5221 ggtgccttta ccaaatgtcg gttcacccac aaccagggca cgaccgtaat cctgcattgc
     5281 cgcggcaaag atttctgaag ccgaagcact gaagcggtca accagcacca ccagcgggcc
     5341 tttatagaag acctgtccgt cggtgtcgct gtcttcacga accttgccgt tgttatcgcg
     5401 gacctgaaca atcgggccgg atggaatgaa cagaccggag agcgatacgg cttcagtcaa
     5461 cgccccacca ccattgctac gcaggtcgat aatcacactg ctgacattct gtttttccag
     5521 tttctgcaat tgcactttga catcgtctgt caaacctaca tagaagcccg gaatatccag
     5581 cacgccgact ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag
     5641 acgaatacgt tcacgggtca acgttaccgt acgggtcttg gtccctttac cagcaggtaa
     5701 aatttccaga cgaactttac tgcccttagg ccctttaatt aaggccacca catcatcaag
     5761 acgccagcca atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc
     5821 accaacgctg atagctttgc tcttcgctgc cggacctcct gccaccatcg aattgataac
     5881 ggtgtagtca tcatccattt gcagcactgc gccaatacct tccagcgaca aactcatttc
     5941 agtgttgaac tgttcggtat tacgcgggga aagatagttg gtatgcgggt cgatttcacg
     6001 cgcaaacgcc gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg
     6061 aatggcaaat ttgtagcggc gagtcagggt ttcacgaatt tccttatccg tttttcctgt
     6121 cagcttcagg cttaactcgt cgaatttgac tttactgtcc cacagcgcgt tcaactcagc
     6181 ctcgtttttc ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa
     6241 atccatcggc ttttccagta ccgacaaagc gtactggtaa cgttcaaaac ggcgcttttg
     6301 cgccagattg tagagatcgt agaaaacgtc gagtttacct gaacgcagtt catcgcctaa
     6361 ctcagttttc tttttcgcga actgttcaac atcgcttgcc agcagcacat tgtggctgta
     6421 atcgagcaga ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat cgaggtcgaa
     6481 ctggcgataa tgagaacggg tgaagcgcga cgttacgcgc tcactcaccg tcgcatgctg
     6541 cgtctcttcc tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt
     6601 ctggcctgct attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc
     6661 tggcccggcc tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg
     6721 aattcagctg gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt
     6781 tttgacccgc tttcacctta agggcttgtc cgacagtcag agctgaaatg tcagaaaccg
     6841 gggtgtgctg ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt
     6901 gcgaacgagg tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc
     6961 gttcgcggcg cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg
     7021 cttgctgttc agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag
     7081 catgctcgac atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcgacacgtg
     7141 ttgcaccggg tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg
     7201 atcgcaattg cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa
     7261 aaataccgat tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt
     7321 cggccagaaa cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat
     7381 ttcctgatta caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga
     7441 cgctatcagt aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt
     7501 tgtaatccgt cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct
     7561 cgtcctcgtc tgtaaagcga ccgaagacgg tactatcgat gtcgagaaca ccaataatct
     7621 gatttttcac caccagcggc agaacaattt cagaattact cgccgcatca caggcaatat
     7681 gcccgtcaaa cgcgtgaaca tcctcgatac gctgcacttg attgcgggca accgcagtgc
     7741 cgcatacacc gcgcccaacg ggtatccgga cacaggcaat tttgccctga aatggtccga
     7801 gcaccagtgt atcgtcctca agcaaataaa aacctgccca gtttacgtca gtgagacgct
     7861 catataacaa cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cccgccatca
     7921 gcgcgttaaa gtcgcgattt aaatccgcgt aaaattctgc tttgttcatt atataatcac
     7981 ttggttgtct tacctggaac tgccagccta ttaaaataag cattaaatgc gttaatgctc
     8041 aagatcattc ccatcatggg ttaagattaa tgttaattct tattacattt ggcacgtcat
     8101 ggctcttaac acaccacaaa ttacgccgac aaagaagata acagtgaggg ctatcggcga
     8161 ggaactgccg cgtggtgatt accaacgttg cccgcaatgt gacatgctgt ttagcctgcc
     8221 cgagataaat tctcatcaaa gtgcctattg tccgcgctgt caggcaaaaa ttcgtgacgg
     8281 gcgcgactgg tcgctaacgc gcctggcggc aatggccttc accatgctgt tgcttatgcc
     8341 gtttgcctgg ggcgaaccgc tgttgcatat ctggctgtta ggcattcgta ttgacgccaa
     8401 cgttatgcaa ggcatctggc aaatgaccaa acagggcgat gcgataacgg gggcgatggt
     8461 ctttttctgc gttatcggtg cccccctcat tctggtgacc tccatagctt atttatggtt
     8521 tggtaaccga ttgggaatga atttacgccc ggtactgctg atgcttgagc ggctgaaaga
     8581 gtgggtgatg ctggatatct acctggtcgg cattggcgtt gcttctataa aggtacagga
     8641 ttatgcccat atccaggcgg gtgtcggctt gttctctttt gtggcgttgg tgattttaac
     8701 gacggtgacg ttgtcacatc ttaatgttga agaactgtgg gagcgatttt atccgcagcg
     8761 ccccgctacg cgtagggacg agaaactccg tgtctgtctt gggtgccatt ttaccggcta
     8821 tcctgatcag cgtggtcgct gcccgcgttg ccatatcccg ctacgcctgc gtcgccgtca
     8881 tagtctgcaa aaatgctggg cggcgctgtt agcgtcaatc gttttgttgt tacctgccaa
     8941 cctgttgcct atctctatca tttatctgaa tggtggtcgg caggaagata cgattctttc
     9001 cggaattatg tcgctggcaa gtagcaacat tgcagttgcg ggaatcgtgt ttatcgccag
     9061 tattctggta ccgtttacta aagtgatcgt catgttcact ttactgttga gtattcattt
     9121 taaatgccag caaggtttac gcacacgcat tctgttactg cggatggtga cctggattgg
     9181 tcgctggtcg atgctcgacc tgtttgtcat atctttaacc atgtcgctga ttaatcgcga
     9241 tcagatcctc gcttttacta tgggaccggc tgcgttttat ttcggcgcag cggtaatttt
     9301 gactattctt gctgtggaat ggctggacag ccgcttactt tgggatgcac atgagtcagg
     9361 aaacgcccgc ttcgacgact gaagcgcaga ttaaaaataa acgccgtatc tcacctttct
     9421 ggctgctgcc gttcatcgcg ctaatgattg ctggttggct gatttgggac agttatcagg
     9481 accggggcaa tactgtcacc atcgacttta tgtcggcgga tggtattgtt ccaggccgta
     9541 cgcctgttcg ttatcagggc gttgaagttg gaacagtgca ggatatcagc ctcagcgacg
     9601 atcttcgtaa gattgaagtc aaggtcagca tcaagtccga tatgaaagat gcgctgcgcg
     9661 aagagacaca gttctggttg gtgacgccaa aggcctcgtt ggcaggtgtc tccgggctgg
     9721 acgccctcgt cggtggtaat tatatcggca tgatgccggg taaaggtaaa gagcaggatc
     9781 actttgtcgc actcgacacc caaccgaaat atcggctgga caatggcgat ctgatgatcc
     9841 acctgcaagc ccccgatctc ggttcactga gcagtggttc attggtctat ttccgcaaga
     9901 tcccggtggg taaagtctac gactatgcca tcaatcccaa caagcaaggc gtggtgattg
     9961 atgtcctgat cgagcggcgt tttaccgatc tggtgaaaaa aggtagccgt ttctggaacg
    10021 tttccggcgt tgatgccaac gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg
    10081 cggcactggt taacggtgcg attgccttcg attcacctga agagtcgaaa cctgccgaag
    10141 cggaagatac ctttggtctg tatgaagacc tggcccatag ccaacgtggc gtaataataa
    10201 aactggaact gccaggcggt gccggattaa ccgccgactc gacgccgtta atgtatcagg
    10261 ggctggaagt cggacagctg actaaactgg atttaaatcc tggtggtaaa gtcactgggg
    10321 aaatgaccgt tgatcccagc gtcgtcaccc tgcttcgtga aaatacccgc atcgaattac
    10381 gcaacccgaa attatccctt agcgatgcca atctcagcgc cctgctgacc ggaaaaacct
    10441 ttgagttggt gcccggcgat ggcgagccac gcaaagagtt tgttgttgtg ccaggcgaaa
    10501 aagccctgct gcaagagccg gatgttctga cactgaccct gacagcacca gaaagttacg
    10561 gtattgatgc tggtcagccg ctcattcttc acggcgtgca ggtaggccag gttatcgatc
    10621 gtaaactcac cagcaaaggc gtcaccttta ccgtcgccat cgagcctcag catcgcgaac
    10681 tggtaaaagg cgatagcaaa tttgtcgtca acagccgtgt cgacgtgaag gtggggctgg
    10741 atggcgttga gtttctcggt gccagcgcct cagaatggat caatggcgga atacgtattc
    10801 tgccgggcga taaaggcgag atgaaagcca gctatccact gtatgccaat ctggaaaaag
    10861 cgctggagaa cagccttagc gatttaccca ccacaaccgt gagtttgagt gcagagacgc
    10921 tgccggatgt gcaggcagga tcggtagtgc tgtaccgtaa atttgaagtt ggtgaagtta
    10981 ttactgtgcg tccgcgagct aacgcgtttg atatcgatct gcatattaag ccggagtatc
    11041 gcaaccttct gaccagcaac agcgtgttct gggcagaagg tggggcgaaa gttcagctga
    11101 atggtagtgg tctgactgta caggcatccc cgctctccag agcattaaag ggggctatta
    11161 gcttcgataa cctcagcggt gccagcgcca gtcagcgtaa aggcgacaag cgtattctgt
    11221 atgcttccga aacagcggcc cgtgcggttg gcgggcaaat tacgcttcac gctttcgatg
    11281 ccggaaaact ggcggtcggg atgccaattc gctatctcgg tattgatatc gggcaaatcc
    11341 agacgctgga tctgattacc gcgcgcaatg aagtacaggc aaaggcggtg ctctatccgg
    11401 aatatgtcca gacctttgct cgcggtggta cgcgcttctc agtggtcaca ccgcaaattt
    11461 cggcagctgg cgttgagcat cttgatacta tcctccagcc gtatatcaac gtcgaaccag
    11521 gccggggcaa tcctcgccgc gactttgaat tacaagaggc caccattact gattcgcgtt
    11581 acctggatgg cttaagcatt attgttgaag cgccggaagc cggttcgtta ggtatcggta
    11641 caccggtgct gttccgtggt ctggaagtag gtacggttac cggaatgacg ctggggacat
    11701 tgtcagatcg cgtgatgatt gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta
    11761 acaattccgt cttctggttg gcatcgggtt acagtctgga ctttggtctg acgggcggcg
    11821 tagtgaaaac cggtaccttt aaccagttta tccgtggcgg catcgccttc gccacgcctc
    11881 cgggtacgcc actggcaccg aaagcccagg aaggcaagca cttcctgttg caggaaagtg
    11941 aaccgaaaga gtggcgtgaa tggggaactg cgcttcccaa ataatgccca ctgctccggc
    12001 gtgcctgcgc cggagcgttt atgctaaact gcgcgcctgt ttttttgcca gtggtatatg
    12061 ctcgtggccc aacacaccgt ttatttcccg gacgcctttc tgacacaaat gcgcgaagcg
    12121 atgccttcga cgctctcttt tgatgatttt cttgccgcct gtcagcgccc gttgcgccgc
    12181 agcattcgcg ttaatacgct gaaaacctcc gttgctgatt tcctgcaatt aactgctcct
    12241 tatggctgga cgcttacgcc aattccgtgg tgtgaagaag gtttctggat tgaacgcgac
    12301 aatgaagatg cattgccgtt gggtagtacc gccgagcatt taagcggcct gttttatatt
    12361 caggaagcca gttcaatgtt gcctgttgcc gccttgtttg ctgacggtaa tgcaccacag
    12421 cgggtgatgg atgtcgctgc tgcaccaggt tccaaaacga cgcaaattgc tgcgcggatg
    12481 aataacgaag gggcaatcct tgccaatgag ttttccgcca gtcgggtaaa agtgttacat
    12541 gccaatatca gccgctgtgg catcagtaat gttgcgctca cgcattttga tggccgcgta
    12601 tttggtgcgg cagtgccaga aatgttcgat gccattttgc tggacgctcc ctgctccggc
    12661 gaaggcgtgg tgcgtaaaga tcccgatgcg ctaaaaaact ggtcaccaga aagcaatcag
    12721 gaaatcgcag cgacccaacg agagctgatc gacagcgcct ttcatgcatt acgtcctggc
    12781 ggtacgctgg tttactcgac ctgtacctta aaccgggaag aaaacgaagc cgtttgcctg
    12841 tggctgaaag agacttatcc cgacgcagta gagtttttac cgcttggcga tctcttccct
    12901 ggtgcaaaca aggcgctgac cgaggaaggc tttttgcatg ttttcccaca aatttacgac
    12961 tgcgaaggct tcttcgttgc tcgtctgcgt aaaactcagg cgattcccgc cttacccacc
    13021 ccaaaataca aagtgggcaa tttcccgttc agcccggtga aagatcgcga agccggacaa
    13081 attcgtcagg cggctgcaag tgttggctta aactgggatg aaaacctgcg actctggcag
    13141 cgtgacaaag aactgtggtt gttcccggtg ggcattgaag ccctgatcgg taaagtccga
    13201 ttttctcgct tggggattaa acttgccgaa acgcacaaca aaggttatcg ctggcagcat
    13261 gaagccgtta ttgcccttgc ctcccccgac aatgtgaacg cttttgaact gacaccgcag
    13321 gaagcggagg agtggtatcg cgggcgcgat gtatacccgc atgccgcgcc agtggcggat
    13381 gatgtattgg ttaccttcca gcatcagccg attggtttag ccaaacggat tggttcacga
    13441 ctgaaaaaca gctacccgcg tgaactggtg cgcgacggga aactttttac cggtaacgcc
    13501 tgacagcgca caaaaaaaag cgcacttttt gactggcaca ttcaactgcc tcaactaggc
    13561 tgaaaaatgg tgcgatcgga ctggtcgtac cacaaccggc agctaaatgg agagcacgaa
    13621 gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac gacggcgaat tacacggcga
    13681 atcgccgggt gatcgaacgt taaccattcc ttgtaaatca gaccccgatt tatgtatgca
    13741 actggatgcc tgggatgctg aaaccagtat ccctgccctg ctaaatggcg aacactctgt
    13801 cctttaccgt acccgttacg atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc
    13861 caaaaagaac ccgtcggcat ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc
    13921 tctcctaaac ttaacggtac ggcaccacac ttcggggatg aaatgttcgc gctggtactt
    13981 tttgtttgct acctggatgg cggttgtgaa gatattgttg tggatatcta caacacggaa
    14041 cagcagtgtc tctattctat gagcgatcaa ctgatccgtc atggcggttg ttttccgatt
    14101 gaggatttta tagatggttt ctggcgacct gcacaggagt acggtgattt ttaattattg
    14161 caattgcaca agagtcagtt ctcccccaaa gacagcaccg gtatcaatat aatgcaggtt
    14221 gccaatatcc acgcgatggc gcaaaggtgt atgaccaaac cagaaatgat cagcacctgc
    14281 aattccctgc cctttttggt gttcacctaa tcgcgagcgg ctccacaaga tctgatgcaa
    14341 atcgacgtcc ttttgccatt cataaacatc atctggataa tcggcatgag caataacatg
    14401 tttgccggtg cgactgtgta cttcaagaat aaagggcaaa tgctgacatt tttccagcgc
    14461 cgttttcgct tgtttctggt gattatctgc cagcgcaata aaccagtcgc cgccattcat
    14521 caaccataaa gacatctgcc gggatgccag cgcatccatc gccatctgtt catgattgcc
    14581 tcttaccgca cgaacccaat gttgttccag taactgcagg caacgtaaac tttgcggccc
    14641 acgatcgata acgtctccca ctgagataag taaatctcgc cacggatcaa aacgacaatg
    14701 ccataatttg cggcgcaact gctcaagaca accgtgtata tcgcctgaaa gccagatatg
    14761 tcgccattga tgacccgcaa ttctctgata aacgggcgca ggctgtttca tcaatatttt
    14821 cctcccgcgc taaagatcac ataatcttaa caagaatgtt aaaaaacgct ggactcagac
    14881 agtagagtgt gtgttatggt tgactataaa gtcagcgaag ggaatgcttc tggcttttaa
    14941 cagataaaaa gagaccgaac acgattcctg tattcggtcc agggaaatgg ctcttgggag
    15001 agagccgtgc gctaaaagtt ggcattaatg caggcttagt tgccttgctc tttaagaata
    15061 gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc
    15121 atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga actgaggcgg ttttttattg
    15181 gaaatcaaaa ggctatttta ggtaattaac agagtttttc agctcgctct ataaacggtg
    15241 ccagactcat tttttcgccg ggattgttag gatcatcaat ctgaatcacc gaaatgggtt
    15301 gggctttagt cttcccactg gcaacttcct tttgtgcgat atcgtttaaa ggatactgca
    15361 cgagggtact tgggttaatg acatacaaag cattacccgg tcggcaagtc agcatcacct
    15421 cttcgcgatt aaacgcccat ttgtctttac ccacttcaaa acggctgacg gtaatcacct
    15481 gcggtgccgc cagcgcagct gcagaactgg tgagtaacag aaacgccaga atactttttc
    15541 tcatcatttt cgtaatccat caaaaaggtt cgagagtcgc aagcaggctg atgatcacca
    15601 gcactaccgc acctatcgcc cattcgagtt tagtcatcca gacaaaccag ggagccgcac
    15661 gatcttcatc ctgcctcatg cgcggtacaa gaacataacg attagccagc gcgatgacca
    15721 ccataatcat caccaggatg gctttgagca ataagagttg gccccagtaa gtcgtaagcg
    15781 tggggggaaa tccggtaatc aacaatgcat taagcacgcc gcttgccagt acgccgatca
    15841 ccgcaaaatg cccgcaccag gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac
    15901 ggcctttgat gagttgcata caccagagca ccggcagtaa tcccccaaac cacgccgccg
    15961 cacagatcag gtgaattgcg tgattagtct gatggatttt cgccgttacc ccttcattca
    16021 acgtcgcatg cccgactccc gccagcagaa taaattgcgc ggtagtgagc ataaacagca
    16081 aacgtggcat attccgcggt tgcataaggg cgacgatcaa cgttacgagg gcgagaacaa
    16141 tttgccataa ccagacgcca ccaaactgcg tttgcaaaac agccaaccaa atgtttggcg
    16201 aaaatacatc tgaccatccc gctcccatca acccgccctg aacggcgagc attgcagtgg
    16261 cactgatcaa actccagacg gcagcgtgct gttgtaaacg cagaaaacgc ttcgtcagca
    16321 agcgacgaat cgttaagggg gccagccagg caccgtacat tgcgaagcca aaaaccagca
    16381 tcagcgaggt gaaatggata aatcgcagcg cgatccaggt aaacgccagc atgatttatt
    16441 tcacactaaa ggtgtaatgt cctttcgttt tatgcccatc caccgaaaca acatgccagt
    16501 caacggtgta ggttccgggt ttcagcgaat cggccagtgg aacaatcagt tgcttttggt
    16561 cctgctcatt ccgcttcgcc ggtaatgttt taatattttc gttttttggc cccgtgattt
    16621 ttgcaccact gaatcctgtt tcaacgcctt ccgagaagtt taaagtgatt gcctgcggag
    16681 ctgctgtcac ttgtgcattt gccgcaggat actgatgcgt taaatgcgca tgtgcccaga
    16741 cggaaggtgt caccaacgaa gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag
    16801 ttgaagccat attatctatt cctttttgta ataacttttt tacagagcat aaccttgtct
    16861 aatgtctgag tcgaggatca tcaattccgg cttgccatca aggctcactc ttagtaactt
    16921 ttgcccgcga atgatgagga gattaagaat gctgaagaat ctggctaaac tggatcaaac
    16981 agaaatggat aaagtgaatg tcgatttggc ggcggcaggg gtggcattta aagaacgcta
    17041 caatatgcca gtgatcgctg aagcggttga acgtgaacag cctgaacatt tgcgcagctg
    17101 gtttcgcgag cggcttatcg cccaccgttt ggcttcggtc aatctgtcac gtttacctta
    17161 cgagcccaaa cttaaataaa acttatacat agttacactt tcttacataa cgcctgctaa
    17221 attatgagta ttttctaaac cgcccccata atttgcagtc attttgaaaa ggaagtcatt
    17281 atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa
    17341 caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta
    17401 gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct tcctgcccca
    17461 cccgatctct cgctattaga cccgctttgt tatgccgcaa cgacctggcg aatgaccatc
    17521 attgccggcc ttcccgttga atataacgat cgctttattc gtggaattgc ggtattcgca
    17581 ccgtggcgaa aaacgccagg gatctaccat cagagttatg gtgcatgtct gggccgtcgt
    17641 tccagaacca tcacggtggt cgatgaacaa ccgcaaggta tggatatgga cccgacctgt
    17701 tcactgttta cgacagggca atgtctggga gaacccgacc tgctggcgtc ggcccgtcgc
    17761 ttacaatttt tttcacatca gtactcgatt gctgtgctga tggcaaatgc ccgtggtaac
    17821 agtgcactat gggatgaaca tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg
    17881 ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt acgctaggct
    17941 ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc ggtttgcagg
    18001 gagggatcgt tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc aatcccatga
    18061 gccacctggt gcgtcccgat cgtcctatta gtccacaggc gatggcgatt catcgcatca
    18121 ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccgcac tattacggta
    18181 gtgaatggta tgtcgcgcat aacgccagct ttgaccgccg cgtactgcct gaaatgcccg
    18241 gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gccagggatc aagtacagca
    18301 atatggcgtt atataaaaca cggaagctca atgtacagac gccgccgggc ctgcatcatc
    18361 accgcgcgtt gtatgactgt tatatcaccg ctgcgttgct tatcgatatt atgaacacct
    18421 ccggctggac ggcagaacag atggccgata tcaccggacg tccatcgttg atgacgactt
    18481 tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct
    18541 atctgcgctg gttatttaat aacctggaca gcatgagccc ggagctgcgt ttaacactga
    18601 aacattatct ggaaaatact taatccgcag gcgtagcggg taatgttccc tgcgccagcg
    18661 cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcga ccagatttac
    18721 cgccatgacc tgagtccatg tcggtacaga gcaataaaag atggtcatcg gttttcagct
    18781 cgcgcaattt agcgacccat tttgccggtt cccaatattg cacctgagaa tcgtgtaaac
    18841 cggtcgttac cagtaaatgc ggataagcct gtgcggtgac gttgtcatac gggctgtagc
    18901 ttttcatata ctcgtaatat tgcggatcct gcggattccc ccactcttca aattcgccgg
    18961 tggtaagagg aattgattca tccagcattg ttgttacaac atcaacaaac ggtacctggg
    19021 cgataacgcc gtggaacaat tccgggcgtt gattaatcgc aacgcccatc aacatacccc
    19081 ccgcactccc gcccatcgca taacagagcg aaggagagcc atagcccagt tttaacaatg
    19141 catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat ttaccgtcct
    19201 cgtaccattg ttgccccagc tcaccgccgc cacgaacatg gacaatggcg tagacaaatc
    19261 cacgatccaa caaactcaag cggctaaaac tgaaatcggc atcaatactt gcgccgtaag
    19321 aaccgtagcc atacaccagc agcgggttgt gtcctttgcg aaaatgtttg cgatgataga
    19381 ccagcgaaac cggaacttcg acgccatcac gggcgactat ccacaggtgt tcactgcggt
    19441 aattcgccgc atcaaaacca ggaacttccg tttgttttaa tacacgacgc tcaccggtat
    19501 ccatatccag ttcaaacaaa gtatctggtg tggtcatgga agaataacca taacgcaatc
    19561 gcgcggtttc aggttctgga ttgtaggcaa tccaagtcac ataggccgga tcatcaaagg
    19621 caatgccaat gacttcccgg gtcttgcggt taatttgccg caaactggtt aacccgcgct
    19681 gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc ataatgttat
    19741 cacgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta cggtataagc
    19801 caaagttttt accgtggcgg ttggaacgca gataaaaccg atgctggtag tgatcaaggc
    19861 tgtattcgtg atctttgcgg cgaggcagaa aaacaaacgg ctcggcatcg gccatttccg
    19921 cgtccagcag gcgaacttca ctggtggtgg cgctggccag atgaatgacc acataatgct
    19981 tcgaggtcgt tttatgcagg ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt
    20041 tatcttgcga tgctggcgta ccgatggcgt gacgccggac ctgataaggg agcagcgtca
    20101 ctggatgctt gcgaacatag tagaaagtcc aggagtcatt tgcccagaca aagctgggtt
    20161 caacgttatc cagcagttcc gggtaccagt tacctgtttc cagattacga aaacgaatgc
    20221 cgtactgccg tcgggaaaga aaatcttctg ccagtgccat aatggtgtta tctggcgtaa
    20281 tcgccattcc gcccatcgaa taaaactcac tatgagccgc gcgcttgttg gcatcgagca
    20341 atgtttccca ctcgtcccac tcttcactga acgccgattg acgctggtag atagcatatt
    20401 cacagcctgg ttcataaata tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa
    20461 cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt
    20521 gcgaggccat cacccgatga ccgtaactat tttcttgttg cagatagtcc aggacttccg
    20581 gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa
    20641 gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg
    20701 aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg gtagtcattg
    20761 cttagatgat gacaggtaat ggcgcggata tcgactgtta tgcagacaga gaaaatcagc
    20821 ctgttcaaat ggctgtgcgg ttctggataa cccgaaatag tcaacttcag gctatccaga
    20881 gagcggaatt actccgccaa agtacgtttt tgctgttcga gatcgcgttc aatgccttca
    20941 cgaacgtcct gggggatttt cagcgcatca cccagtgcat tcaggtaact gcgttccata
    21001 aaatggtcaa tatcaatagc cgcgcagctc aggaaataga tttccagcgc ctcttcttca
    21061 ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg
    21121 agtacacgcc ccttctcttc cacaccggct tctcgcaatt gctggtcgat tgccgcacgt
    21181 tctttggcat cgatatggcc atcactttta gcggcaaaga ccagcgcaag gatcaaacgt
    21241 tccgtacgct catccagcgg cgtactttgc gcgccaaact gcggttcatc ctgatgcgcc
    21301 gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc tccgccgcca
    21361 accagtaacg cgtttgtgcc atatttcgta agaagtttac gtgctgattt atttgcgacc
    21421 agcagcccag ccagtccgcc taatgcgcca ggcactaaaa gtttgcccaa tccctgatcc
    21481 gcagacgagg aggtagaaga acggctttgc ccaagcagag attgcagttg atttaaccag
    21541 ttagccatat ttgctcctca ataaccattt ttagtgcctg tagcatagcg gatgaggatg
    21601 tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag gccgttattc
    21661 aaagaaattc gcgccaggta aggtgttcac tgtatataag gtgataaggg tattttaggt
    21721 gaaagtagtt tgacattttg agtggaactg tttgatgctg attgcccagt tcgacgctac
    21781 tgcgtcttct caggactacg attgtgttgc gttgtagggc ggacaaggca ctcgcgccgt
    21841 atccgccaac ggtcaatcga attatcagaa gccattaacg ccgctgatat tccgccattc
    21901 ccgctttgca gtccacgctg acctggtaat gaatatcggc acttttacca cgcacggtta
    21961 gcggtactga ccatttatca tctttcccct gaatgtcctg caaactgacc catgccacgg
    22021 ggtcggcctg accgacaatt ttttgatcat cagcccagcg cgccacgcga ttttgttggt
    22081 aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac tttgggaaag
    22141 tgaccgactt gctggtttca ttattggcag cgaaaactga tgcgcaggca gaaaccaaca
    22201 acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa tgattcagga
    22261 gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt gaggcatctt
    22321 cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt ttcagaatgg
    22381 tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt tgctcatctt
    22441 ccatttgcac tggagaattt gtcagccagg tgtcaagcag atcggccgta tcaagcatct
    22501 tgtcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg ccctcacgaa
    22561 tgactacgta tttaacttca accgccattt gcagcctctc ataataactg tgattttata
    22621 cagtatattt cttttcagtt gggaaatcaa catcagcaat aaagacacac gcaaacgttt
    22681 tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg
    22741 cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag
    22801 tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg
    22861 caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat ggtgatgcat
    22921 tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg
    22981 ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg
    23041 caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc
    23101 tgcccacttc cacttatcgt tttgccgata gcgaaaacct tttccgcgaa gcggttgccg
    23161 ccattggcta cccctgtatt gttaaaccgg tgatgagttc ttccggtaaa gggcagacgt
    23221 ttattcgttc tgcagagcaa cttgctcatg catgggagta cgctcagcaa ggtggtcgcg
    23281 ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc
    23341 taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag
    23401 atggcgacta tcgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg
    23461 cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatgggctg tttggtgtcg
    23521 agctgtttgt ctgtggtgat gaggtgattt tcagtgaagt ctcccctcgc ccgcatgaca
    23581 ccgggatggt gacgctaatt tctcaggatc tctcggagtt tgccctgcat gtacgtgcct
    23641 tcctcggact tccggttggc gggatccgcc agtatggtcc tgctgcttct gccgttatcc
    23701 tgccgcaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag
    23761 atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt ctgggggtag
    23821 cactggctac tgcagagagt gttgttgacg ccattgaacg cgcgaagcac gccgccggac
    23881 aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc gatcctcgat
    23941 cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca gcttagtaat
    24001 gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac caccgatgca
    24061 cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg
    24121 gcagaaacgg acctgagaga acggacccgc gatcgcctgc agggctttca caccgccgtt
    24181 agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca gcatcagttc
    24241 ggaaacagtg ctgatccccg gaatcagagg aatagtccct tcggtagcag ctttcagcag
    24301 cggctcggtc agacccgggc taattgcaaa ctgtgcacct gcttcagtga cttctgccag
    24361 ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt ctttggcgat
    24421 agcacggata gcgtcaactg cgcactcggt acgcagagtc acttccagaa cgcgcacccc
    24481 accagcaacc aacgcttttg ccatcggcac cgcgtgttcc agttttttta ccacgataac
    24541 cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt ttttcatcag
    24601 agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg
    24661 ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg
    24721 tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt cgtctaccag
    24781 cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc gcacttttgc
    24841 cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca ctttacctga
    24901 agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt ccaataatac
    24961 accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg gcccctgatg
    25021 acggacaaca acgacacaat cgcggtccag taaacccgct tcaaaggccg gcataacgtc
    25081 atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcta ccggcacggc
    25141 agaggttttc ataaccgctc ggcccaggtt accgcttaac acttttgtcc caccatgatg
    25201 agagaaaggt tgttcgaagg acgcgatcac attgttgtcg agtgattttt ctgccccttc
    25261 ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag acagaccaaa
    25321 gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt cacgtaccag
    25381 aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt tcgggtagag
    25441 acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt taatctgaat
    25501 accggctgcg cgcgccatcg ccactaggtg catggtgtgg ttagtggaac caccggtcgc
    25561 cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac cgatcggcat
    25621 ccattcatta ccattaccgg tcatgcgcgt aacctggcgc gcagctgcag cggtcaaagc
    25681 atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca tccccataaa
    25741 ctccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc ccggcgcatg
    25801 ataagacgcg gcttctgact ccagtaatgc catgcggtcc actttacctt cggcataaag
    25861 ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc cagacggcac
    25921 aaacaccgca ggcaaatgac caaacgacag ggctgccatg gtcagacctg ggacaatctt
    25981 gtcgcacaca ccgagaaaca gagcaccatc aaacatgtta tgggacagcc ccaccgccgc
    26041 agacatcgca atcacttcgc ggcttaacag cgacaattcc attccatcct gcccctgggt
    26101 gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg
    26161 cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgagcgg agagcatgtc
    26221 gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag aggctttgtc
    26281 ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact gcgaacgatg
    26341 aacggtcgaa gttttcgctt gttctatccg ggtgaggtaa gcagagcgag tctcgcgcga
    26401 acgttcaatg attcgatttg ttacgcgtaa caattgtggg ttcataaagg ctcctgaaat
    26461 tgagttgtca gagcaggatg attcacaacg cgtttcatac atggaaatta tgattgaaac
    26521 gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg
    26581 tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca
    26641 tgttaccggt aaaataacca taaaggatga gcgcagatat tactcaaact cattccagga
    26701 acgaccatca cgggtaatca tcgccactga ggcaacgggt ccccaggttc cggcctgata
    26761 cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta cccatttcca
    26821 ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga ataccacgca tggtttccag
    26881 cagcaaacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg aatagctcag
    26941 atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt tattcagtac
    27001 ctggatatcc acgccttcat caggttgcag acggatagtc agtttatttt gcggtagatc
    27061 ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca cgacttcaga
    27121 acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg cccagcgcca
    27181 gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc
    27241 ctcttcttcc agatagcccg gcactttttt gccctgagcg aagcccgcag tatattgccc
    27301 gcgtacggtt ttttcgcgca cattggagcg gtcgatgcgg cgcagagact tcagtacttt
    27361 cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca tcgcaatcat
    27421 gcaaagaatt tgcagcaggt ggttctggat catgtcgcgc atctgaccgg ctttatcaaa
    27481 atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa catgatcaat
    27541 ggtgcgattg tcccagttgt tcacaaacag agagttagca aaacgcagcg ccaacaggtt
    27601 cagcactgtt tctttaccaa gatagtggtc gatacggtaa acctggcact cctcgaagta
    27661 ttcgccaacc tgatcgttga tttcctgcga ggtcgccagc gacgtcccca gcggtttttc
    27721 catgactacg cgtgccggtt tagcattcag ttttgcctca ccaagccctt tgcaaattgc
    27781 gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt tttgatccag
    27841 catcgcgccg agacggctga atgcagcagt gtcattgaca tcaagattac aaaaatccag
    27901 acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga aagtttcgag
    27961 cgcctcgcgg acaactttgg tataagccgc tttatcccag tcagcacgcc ctacgccgat
    28021 aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg aaggcagcaa
    28081 tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt
    28141 ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa gccagggtat
    28201 acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa actgtaaacg
    28261 cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa
    28321 tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa aaataacaac
    28381 ttttttatct gcttttgtca ttaacacagc acacaggcgt aatatctgac aaaactgcat
    28441 ttcgatttct ttcagagcgg aaatcgtcat tacccgtgag tctctttaca tcatgaatat
    28501 gctggaaaaa atccagtctc agctggaaca tttgagcaaa tctgagcgca aagttgccga
    28561 ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc tggcgctgga
    28621 agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca cacgcggttt
    28681 tcctgatttt aaacttcatc tggcacaaag tctggcgaat ggcactccct atgttaatcg
    28741 caacgtcaat gaagatgaca gcgttgaatc atacacgggg aaaatatttg agtccgcaat
    28801 ggcaacgctc gatcatgtcc gtcattcact ggataaatct gccatcaacc gcgccgtcga
    28861 cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggttcttcag ccgccgttgc
    28921 ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact ccgatgatat
    28981 cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg tgctgatttc
    29041 tcacacggga agaacaaaaa atctggtcga gctggcgcag ctggcacgcg aaaacgacgc
    29101 tatggtaatt gccctcacct ctgcgggtac cccactcgcc cgggaagcaa cgctggcaat
    29161 taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc gacttgcaca
    29221 gctgaccgtg atagatgtgc tggcgacagg atttactttg cggcgcggta caaaattcag
    29281 agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata agcagttact
    29341 taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata cgacatccga
    29401 atgagattaa tttatcgcca tcacggcgtt atttcattcg gatttcatgt tcaagcaaca
    29461 cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga actaaaatcg
    29521 ttaccacgtt aggcccggca acagatcgcg ataataacct tgaaaaagtt atcgcggcgg
    29581 gtgccaacgt tgtacgtatg aatttttctc acggctcgcc tgaagatcac aaaatgcgcg
    29641 cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc
    29701 tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc ctcaatattg
    29761 gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg
    29821 gtatcgacta caaaggcctg cctgctgacg ttgtgcctgg tgacatcctg ctgctggacg
    29881 atggtcgcgt ccagttaaaa gtactggaag tccagggcat gaaagtgttc accgaagtga
    29941 ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc ggtttgtcgg
    30001 ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg attggcgtag
    30061 attacctggc tgtctccttc ccacgctgcg gcgaagatct gaactatgcc cgtcgtctgg
    30121 cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt
    30181 gcagccagga ggcaatggat gacatcatcc tcgcctctga cgtggtaatg gttgctcgcg
    30241 gcgacctcgg tgtggaaatt ggcgatccgg aactggtcgg cattcagaaa gcgttgatcc
    30301 gtcgtgcacg tcagctaaac cgcgcggtaa tcacggcgac ccagatgatg gaatcaatga
    30361 ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc gttctggatg
    30421 gtactgacgc tgtgatgctg tcggcagaaa ctgctgctgg tcaatacccg tcagaaactg
    30481 ttgcagccat ggcgcgcgtt tgcctggggg cggaaaaaat cccgagcatc aacgtttcta
    30541 aacaccgtct ggacgttcag ttcgacaatg tggaagaagc cattgccatg tcagcaatgt
    30601 acgcggctaa ccacctgaaa ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta
    30661 ccgcgctgat gacctcccgt atcagctctg gtctgccaat tttcgccatg tcacgacatg
    30721 aacgtacgct gaacctgact gctctctatc gcggcgttac gccggtgcac tttgatagcg
    30781 ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgtgat aaaggttact
    30841 tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc gtgggttcta
    30901 ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc
    30961 tcatccggcc tacagttcaa tgataattca acagatttcg aatcttctga agcaaacttg
    31021 aacttatcat cagtcgaagg cctctcctcg cgagaggcat tttttatttg atgggataaa
    31081 gatctttgcg cttatacggt tggatttcgc ccggtttgcg agttttcagc aattttaata
    31141 tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca ttcatccgcc
    31201 gcgcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc acctgaatcg
    31261 tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca
    31321 ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag aaatcaacaa
    31381 attcgctgtg ttctgggcca tgatcctgat cgggtaaata atatccccag taaccctgac
    31441 gtaccgactg gatgaatggt ttaataccat catttctcgc atgcagacga ccaccaaagc
    31501 gacggcgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta tggaacatcg
    31561 ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg gcccaaccgt
    31621 gcggcaccag aaaaatcact ttctcgttat tacgccgcat ctcttcgatg atctccagcc
    31681 cttgccagtc aacgcgcggc tgaattttct ccggcccgcg tattgccaac tcagccatca
    31741 ttaccatcgc ttgcggcgcg gtggcaaaca tctcatctac aatcgcttcg cgttcagctt
    31801 cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt gagctttttc
    31861 ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac tttggcggcg
    31921 ttaaagcgat acccgccatc gctgctacgc ccagccatgc tccccagtag cgcgggtggc
    31981 gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt tccatgcttt
    32041 tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcgca acttgccccg
    32101 caccaaataa aaaagccggt actgactgcg taccggctgc gaatggatgt taattaatca
    32161 aaccgtagct gcggcacaat ctctttggcc tgtgccagga attcgcgacg atcggagccg
    32221 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
    32281 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
    32341 cggtcgccac gtttcacctt ctgtcccggt ttcaccagga tcttgcgcaa gtgcatataa
    32401 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactacgt
    32461 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccttg tggcatggca
    32521 aaatcaacac ctctgtgtgg cgcaacgcga ccggtcaccg gattagtacg acgcgggtta
    32581 aagttagatg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
    32641 ccagtaccgt tacgatcata gaatttgcca tcttcagcgc ggattgcata ataatcttta
    32701 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
    32761 tctcgtgaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
    32821 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg
    32881 ctggcaacaa agcttccccc gacggtacct ttcagcagat tgttgaccca ctctccttgc
    32941 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
    33001 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
    33061 agttgttgac cgattttcag gttacgcaat tctttgtcgg cagcagccag ttgggtgata
    33121 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
    33181 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
    33241 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
    33301 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcacg gtgataaaca
    33361 tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
    33421 tgtggtcgcg gtaaattatt aaacgctagg gcgacagagc gggctatctg ttgcacgtaa
    33481 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgattcagg
    33541 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc caacgttccc
    33601 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
    33661 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
    33721 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccgaactgt
    33781 ttttcgaagt agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
    33841 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
    33901 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
    33961 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
    34021 tgatcttcgt cacttttttc cgcgtgatcg tgatcatcat catcgtcgtg aatacttttc
    34081 atcagcagcg gtttcacatc ttctagctgc gcaatcgtta cctgtttcgc ttctggtaat
    34141 ttacttaccg gtttttgcat gaacgcttcc atctccgggc caacccaaac gactaagtcc
    34201 gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc tgaagcgccg
    34261 tcaggcagta aaacctgcgt ttctgttacc ccatcagcaa tggcagaagc gatgaaccca
    34321 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc gccccagaga
    34381 gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
    34441 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
    34501 acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
    34561 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaacg
    34621 gcgcaggtaa gtcgacactg gtacgggtag tgctcggact ggtaacaccc gatgaagggg
    34681 ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca
    34741 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggcaca cataaagaag
    34801 atattttgcc tgcactgaaa cgtgtccagg ccgggcatct gattaacgca ccgatgcaaa
    34861 agctctcggg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgaccgc
    34921 aattattagt gctggatgaa cccactcagg gcgtggatgt gaatggtcag gtggcgttat
    34981 atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg gtatctcacg
    35041 atctgcatct ggtaatggca aaaaccgatg aagtgctttg cctgaatcac cacatttgtt
    35101 gttccggcac accggaagtt gtttccctgc atccggagtt tatttctatg tttggtcctc
    35161 gtggtgctga acaactgggt atctatcgcc atcatcataa tcatcgtcac gatttacagg
    35221 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
    35281 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
    35341 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggcg tcgcgtttgg
    35401 tttgttgctg gacgtgaatc cattctatgc ggtgattgca gttacgctgc tgctggcggg
    35461 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
    35521 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt
    35581 tgatttgatg gcttacctgt tcggtgattt actggcagtg acgccagaag atctcatctc
    35641 tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
    35701 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa
    35761 attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat tcgtcggcgc
    35821 gttgattatt acttcactgc tgattattcc tgctgctact gcacgtcgct ttgcccgcac
    35881 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtga ctggcggttt
    35941 aaccttttcc gcattttacg atacacctgc aggcccgtcg gtggtgctat gcgcggcact
    36001 gttatttatt ctcagtatga tgaaaaaaca ggccagctaa tctttcgctg aacatatttg
    36061 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt aggcctgata
    36121 agacgaacag cgtcgcatca ggcacactgc caatgtcgga tgcggcgcga gcgaccaatc
    36181 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
    36241 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat caaataaggt tccagtacat
    36301 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
    36361 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
    36421 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
    36481 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
    36541 taccgcgcgc acgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
    36601 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
    36661 gcacaatacc aaaacgatcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
    36721 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
    36781 tcatgatatc cagttggtaa tcttccattg ccggatacaa cacctcttcc accactggtg
    36841 aaagacggtg gatctcatca ataaacagta catcgtgtgg ttcaaggtta gtgagcattg
    36901 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
    36961 tttcattggc gacaatattg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
    37021 atagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgcg
    37081 aacgaacctg cggctgacca acatactctt ccagtaattt agggcggatg gcgcgatctg
    37141 ccacatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
    37201 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg
    37261 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagtg ccaccagcgc
    37321 agcaaccgct tcctgttcag catcgtcggt cgccgggctg gcaggagacg tgagtaccag
    37381 gtcggcggct ggcgtaaaga gatcgccatg caaaccttta aatcggtctt tcatttcgac
    37441 aatcaagcgt tcggcggttt ttttgccaat acccggcagt ttcaccagtg cccccacttc
    37501 ttcacgctca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
    37561 caacttcggg ccgacgccgt tggttttgat caactctttg aacaacgtgc gctcttgttt
    37621 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
    37681 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
    37741 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
    37801 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
    37861 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcaatgatc
    37921 tgaaaaatgg cgcagtataa tttattctac aggttatatt ggaagcaaat atttaaatat
    37981 tacatattca gcgaagaaat gtgtaataaa aatatacatt gcgactcctg aa
//
LOCUS       DAZFRE010000041        37788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFRE010000041 DAZFRE010000000
VERSION     DAZFRE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.041"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(132..467)
                     /locus_tag="ACHKUI_002408"
                     /pseudo
     CDS             complement(132..467)
                     /locus_tag="ACHKUI_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(622..697)
                     /locus_tag="ACHKUI_002409"
     tRNA            complement(622..697)
                     /locus_tag="ACHKUI_002409"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Thr,seq:cgt)
     gene            complement(812..2065)
                     /gene="proA"
                     /locus_tag="ACHKUI_002410"
     CDS             complement(812..2065)
                     /gene="proA"
                     /locus_tag="ACHKUI_002410"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6598594.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVIIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2077..3180)
                     /gene="proB"
                     /locus_tag="ACHKUI_002411"
     CDS             complement(2077..3180)
                     /gene="proB"
                     /locus_tag="ACHKUI_002411"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6598595.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3469..4524
                     /gene="phoE"
                     /locus_tag="ACHKUI_002412"
     CDS             3469..4524
                     /gene="phoE"
                     /locus_tag="ACHKUI_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6598596.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4563..4964)
                     /gene="crl"
                     /locus_tag="ACHKUI_002413"
     CDS             complement(4563..4964)
                     /gene="crl"
                     /locus_tag="ACHKUI_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6598597.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5022..6266)
                     /gene="frsA"
                     /locus_tag="ACHKUI_002414"
     CDS             complement(5022..6266)
                     /gene="frsA"
                     /locus_tag="ACHKUI_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6598598.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6358..6816)
                     /gene="gpt"
                     /locus_tag="ACHKUI_002415"
     CDS             complement(6358..6816)
                     /gene="gpt"
                     /locus_tag="ACHKUI_002415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6598599.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7077..8534
                     /gene="pepD"
                     /locus_tag="ACHKUI_002416"
     CDS             7077..8534
                     /gene="pepD"
                     /locus_tag="ACHKUI_002416"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6598600.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8591..9205)
                     /gene="prfH"
                     /locus_tag="ACHKUI_002417"
     CDS             complement(8591..9205)
                     /gene="prfH"
                     /locus_tag="ACHKUI_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6598601.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLSSGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9202..10341)
                     /locus_tag="ACHKUI_002418"
     CDS             complement(9202..10341)
                     /locus_tag="ACHKUI_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6598602.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIEQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10531..10983)
                     /gene="yafP"
                     /locus_tag="ACHKUI_002419"
     CDS             complement(10531..10983)
                     /gene="yafP"
                     /locus_tag="ACHKUI_002419"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6598603.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(10993..11391)
                     /gene="yafO"
                     /locus_tag="ACHKUI_002420"
     CDS             complement(10993..11391)
                     /gene="yafO"
                     /locus_tag="ACHKUI_002420"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO6598604.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11394..11687)
                     /gene="yafN"
                     /locus_tag="ACHKUI_002421"
     CDS             complement(11394..11687)
                     /gene="yafN"
                     /locus_tag="ACHKUI_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO6598605.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(11739..12794)
                     /gene="dinB"
                     /locus_tag="ACHKUI_002422"
     CDS             complement(11739..12794)
                     /gene="dinB"
                     /locus_tag="ACHKUI_002422"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6598606.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12865..13788)
                     /gene="lafU"
                     /locus_tag="ACHKUI_002423"
     CDS             complement(12865..13788)
                     /gene="lafU"
                     /locus_tag="ACHKUI_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6598607.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHLPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            complement(13791..14654)
                     /gene="motA"
                     /locus_tag="ACHKUI_002424"
     CDS             complement(13791..14654)
                     /gene="motA"
                     /locus_tag="ACHKUI_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6598608.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14667..15383)
                     /locus_tag="ACHKUI_002425"
     CDS             complement(14667..15383)
                     /locus_tag="ACHKUI_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO6598609.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(15403..15870)
                     /locus_tag="ACHKUI_002426"
     CDS             complement(15403..15870)
                     /locus_tag="ACHKUI_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO6598610.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAVARTEA
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(15878..16939)
                     /locus_tag="ACHKUI_002427"
     CDS             complement(15878..16939)
                     /locus_tag="ACHKUI_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6598611.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQHQATRPQENDPVAMQALMALLLPQPAAPHQDTPEPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16936..17247)
                     /locus_tag="ACHKUI_002428"
     CDS             complement(16936..17247)
                     /locus_tag="ACHKUI_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO6598612.1"
                     /translation="MEDKCRQLFALVEAMNEALDKQRWRRLPALHQQVMRQFHDYATA
                     ETDATQLCAVKAQLCAAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17252..17644)
                     /gene="fliS"
                     /locus_tag="ACHKUI_002429"
     CDS             complement(17252..17644)
                     /gene="fliS"
                     /locus_tag="ACHKUI_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6598613.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17667..18983)
                     /gene="fliD"
                     /locus_tag="ACHKUI_002430"
     CDS             complement(17667..18983)
                     /gene="fliD"
                     /locus_tag="ACHKUI_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6598614.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAASSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQGAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFSDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19629..20543)
                     /gene="lafA"
                     /locus_tag="ACHKUI_002431"
     CDS             complement(19629..20543)
                     /gene="lafA"
                     /locus_tag="ACHKUI_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO6598615.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            21029..21871
                     /locus_tag="ACHKUI_002432"
     CDS             21029..21871
                     /locus_tag="ACHKUI_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO6598616.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEAQIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(21930..22907)
                     /locus_tag="ACHKUI_002433"
     CDS             complement(21930..22907)
                     /locus_tag="ACHKUI_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO6598617.1"
                     /translation="MQVGLTSSSLATGGALSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRQLQPVLQGEARVTFHSLDLANLVHNPTPGTRMFSVTDGRQTQLSA
                     VMLYEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQADRLAQSLQQGGAGISQMLENINQQRAQMAVQQEKARQLIDGMS
                     RFPQTESAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(22924..23853)
                     /gene="flgL"
                     /locus_tag="ACHKUI_002434"
     CDS             complement(22924..23853)
                     /gene="flgL"
                     /locus_tag="ACHKUI_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6598618.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFDSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(23868..25244)
                     /gene="flgK"
                     /locus_tag="ACHKUI_002435"
     CDS             complement(23868..25244)
                     /gene="flgK"
                     /locus_tag="ACHKUI_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6598619.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTASMTLTFAGTISTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(25434..25733)
                     /locus_tag="ACHKUI_002436"
     CDS             complement(25434..25733)
                     /locus_tag="ACHKUI_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO6598620.1"
                     /translation="MKINGSGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPRHK"
     gene            complement(25733..26833)
                     /locus_tag="ACHKUI_002437"
     CDS             complement(25733..26833)
                     /locus_tag="ACHKUI_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6598621.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVTLHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(26848..27513)
                     /gene="flgH"
                     /locus_tag="ACHKUI_002438"
     CDS             complement(26848..27513)
                     /gene="flgH"
                     /locus_tag="ACHKUI_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6598622.1"
                     /translation="MKNYIWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(27584..28369)
                     /gene="flgG"
                     /locus_tag="ACHKUI_002439"
     CDS             complement(27584..28369)
                     /gene="flgG"
                     /locus_tag="ACHKUI_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6598623.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(28448..29185)
                     /locus_tag="ACHKUI_002440"
     CDS             complement(28448..29185)
                     /locus_tag="ACHKUI_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO6598624.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGVSGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(29185..30387)
                     /gene="flgE"
                     /locus_tag="ACHKUI_002441"
     CDS             complement(29185..30387)
                     /gene="flgE"
                     /locus_tag="ACHKUI_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6598625.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     DAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEPSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPNTPQTIEFQTDAAAPIDLTVDYSSCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(30651..31364)
                     /locus_tag="ACHKUI_002442"
     CDS             complement(30651..31364)
                     /locus_tag="ACHKUI_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO6598626.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTTSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(31364..31795)
                     /gene="flgC"
                     /locus_tag="ACHKUI_002443"
     CDS             complement(31364..31795)
                     /gene="flgC"
                     /locus_tag="ACHKUI_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6598627.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(31798..32133)
                     /locus_tag="ACHKUI_002444"
     CDS             complement(31798..32133)
                     /locus_tag="ACHKUI_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO6598628.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            32215..32952
                     /gene="flgA"
                     /locus_tag="ACHKUI_002445"
     CDS             32215..32952
                     /gene="flgA"
                     /locus_tag="ACHKUI_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6598629.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELRPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNTSSQRVISAMVAESGVVTTVSAE"
     gene            33033..33311
                     /gene="flgM"
                     /locus_tag="ACHKUI_002446"
     CDS             33033..33311
                     /gene="flgM"
                     /locus_tag="ACHKUI_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6598630.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            33324..33752
                     /gene="flgN"
                     /locus_tag="ACHKUI_002447"
     CDS             33324..33752
                     /gene="flgN"
                     /locus_tag="ACHKUI_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO6598631.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEDL
                     LAVNDEITQHYEQLHGHSYQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            33880..34812
                     /locus_tag="ACHKUI_002448"
     CDS             33880..34812
                     /locus_tag="ACHKUI_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO6598632.1"
                     /translation="MSFIECYEPEYVRNFMTQYPDSHLYVRKVWKNEIRQSLALTDIE
                     SCKAVLNDARAFDLQLTYRPVEDNAAELSARDAIVNQAILSTLTLPDLTPELSLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHSQKGTLQAQFAQLPPVPQLARQLMTLLG
                     SFTFDWSILPESPRKASLPLQVTLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQTDGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENAVLIRQQIQAECSADPLLYAFSLLSC"
     gene            34859..37297
                     /locus_tag="ACHKUI_002449"
     CDS             34859..37297
                     /locus_tag="ACHKUI_002449"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6598633.1"
                     /translation="MQTLIKTLATGRVKPFVSVVTPTWNRGTFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPGSFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTNNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAALTDIVSDPLLRNAYLGLHNATHYQAIQHQAIDQIVLLNLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALHLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLNTLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYYTPALSGLSPLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWGAVHARLTEIRHHYCDAFMDGK
                     AGERAALVIVEALG"
BASE COUNT         8590 a        10314 c         9655 g         9229 t
ORIGIN      
        1 taccattgga agatttagaa aagcatgtcc aggagtttaa ggacaaatct catactgatt
       61 atcggaagct tttatcatta tatgattacc aagcatacaa tgtataatta tatacactat
      121 tattgtgatt ataatttgtt actgtgccac tcaagggcaa tatctttgaa ggtgttgttt
      181 aactgcgttt cccgggcaat cttttcctct cgcttcactt ccatcagatc gattccccca
      241 gagatacctc ttttagcttc ttcaagtttt gcaggagcat cagctaaggt gacctcagga
      301 tacacaccta gtgctaacag cttctcttta ccagctacac gatacttgag acgccaatat
      361 tctcctccac caggttttac caggaggtac agaccaccac catcagccaa cttgtaagcc
      421 ttctcttttg gcttggcagt gtctatttga cgggcattga gtttcacttg ggggtatctc
      481 cactaaactg aacagcaaat cccctcaatt gcttgtagag tttggggtac ttaaatagac
      541 gtcaaaagac taaaaggggc attaatatac tgattataag aggtttttaa atacttgagt
      601 agacttgggg agacgttaga atggtgccga taataggagt cgaacctacg accttcgcat
      661 tacgaatgcg ctgctctacc aactgagcta tatcggccct gaaaggacat gttcacgaac
      721 gtgaatcacg gtggacaagg ttaaaactaa ccgggcgatg cgtcaatggc cttgtgaatc
      781 aaatggctac ttttgcatca cccggtttta tttacgcacg aatggtgtaa tcaccaatgc
      841 cgatccactt ataagtggtc agtgcttcca gccccattgg gccgcgcgcg tggagttttt
      901 gtgtgcttac cgcaacttcc gcccccagtc caaactggcc gccgtcggta aaacgcgtag
      961 aggcgttaac gtaaacagcg gaggaatcca cttcgttaac aaaacgctgg gcgttgcgca
     1021 tatcgcgggt caggatcgca tcggagtgtt gcgtgccgtg ttcacgaata tgggcaatgg
     1081 cgtcatccag atcgctaacg attatgacgt tcaaatctaa tgacagaaac tcatcgtcat
     1141 actcttcggc tttaacagcc accaccttcg cggggccagc ctgcaactgc gccagcgcgg
     1201 ctgcatcagc gtgtaacgtc acgccgcttt ccgccatttg tttgcttaat gcgggcagga
     1261 agctatctgc gatgttttta tttaccagca acgtttctac cgtattacat gtgctcggtc
     1321 gctgagtttt cgcgttgaca attactttca gggcttcagc gatctctgcg ctttcatcaa
     1381 cgtaaatatg gcatacgcct ataccgcctg tgatcaccgg gattgtcgac tgctcgcggc
     1441 acagtttatg caaaccagcc ccaccgcgcg ggatcagcat gtcgatgtat ttatccatac
     1501 gcagcatttc actgaccagc gcacggtcag gattatcaat tgcctgcacg gcacccgccg
     1561 gtaagccgca ggatttcagg gcgtcctgaa tcaccgctac cgttgcagcg ttagtgcgac
     1621 aggtttcttt gccgccgcgc aggatcaccg cgttaccggt tttcaggcac agggaagcaa
     1681 catcaaccgt cacgttcggg cgcgcttcat aaatcacgcc aatcaccccc agcggtacgc
     1741 gacgacgctc aagacgcagg ccgctgtcca gtacgccgcc atcgattacc tgccccaccg
     1801 gatcggcgag attgcacacc tggcgcacat cgtcggcaat gcctttcagc cgtgcgggcg
     1861 tcagtgccag acggtcaagc atcgcttcgc taaggccatt ggcacacgcg tctgcaacat
     1921 cctgcgcgtt agcgttgagg atactttcgc tttgtgcttc cagttcatcg gcgatttttt
     1981 ccagcacacg atttttttcg cggctggaga gttgcgctaa tttatacgag gcttgcttcg
     2041 cggcaatgcc catttgttcc agcatcggcc agctccttaa cgggtaatca tgtcatcacg
     2101 gtgaacggca accgggccgt attcatatcc cagtattgca tcaatttctt gcgagtggtg
     2161 tccggcaata cggcgtaatg catcgctgtt gtaacgactg acgccgtggg cgatatcgcg
     2221 accttcgagg ttgcaaatac ggatgacttc accacgcgag aaattgccag tcacgctttt
     2281 aatgcctttc ggcaacaggg agctgccgcg ttccagaatg gcggcagttg ccccttcatc
     2341 taccgtgatt tcacccgcag gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg
     2401 agtcgcctgg gcatggaaca gcgtaccgac ggaaatgcct tccatcacat caccaataac
     2461 gcccggcttg ctgcccgcgg caataatggt gtcgataccc gcacggcaag ccacgtcagc
     2521 ggcctgcaat ttggtactca tgccgccagt tccgaggcct gaaacgctat caccggcaat
     2581 cgcgcgcagt gcgtcatcaa tgccgtacac atctttaatc agttctgcct gcggattgct
     2641 gcgcgggtca gcggtataca aacctttttg atcggtcagc agcaacagtt tatcggcacc
     2701 cgccagaatc gccgccagcg cagaaaggtt atcgttatca ccgaccttaa tctctgcagt
     2761 agcgacagca tcgttctcat taattaccgg aacgatattg ttatcgagca acgcacgcag
     2821 ggtatcacgg gcgttcagga agcgttcacg atcttccata tcagcacggg tcagcaacat
     2881 ctgcccgaca tgaatgccgt aaattgaaaa cagctgttcc cacagttgaa tcagtcgact
     2941 ctgccctacc gccgccagca gctgttttga agcgatagtc gctggcaatt ccgggtaccc
     3001 caggtgctca cgtccggcgg caatcgcacc cgacgtcaca ataacaatcc gatgcccggc
     3061 ggcatgtaac tgcgcgcact ggcgaacaag ttcaacgata tgggcacggt tcaggcggcg
     3121 cgatccgcct gttagcacac tggtgccgag ttttaccacc agcgtctggc tgtcactcat
     3181 gattctctgc cattcaattt taggaaaaaa tgatatcaaa cgaacgtttt agcaggactg
     3241 tcgtcggttg ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag
     3301 tgtagcggca gttgtttacg cttttattac agatttaata aattaccaca ttttaagaat
     3361 attattaatc tgtaatatat ctttaacaat ctcaggttaa aaactttcct gttttcaacg
     3421 ggactctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc
     3481 actctggcat tagtggtgat gggcattgtg gcatctgtat ccgtacaggc cgcagaaata
     3541 tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg
     3601 agtgataatg acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa
     3661 acacaaatta acgatcaact gactggttat ggtcgttggg aagctgagtt tgccggtaat
     3721 aaagcggaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg gttgaaatat
     3781 aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga cgtggaagcc
     3841 tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg
     3901 accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat
     3961 ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa
     4021 aacggcgatg gcttcggcac gtcattgaca tatgactttg gtggcagcga tttcgccatc
     4081 agtggagcct ataccaactc tgatcgtacc aacgagcaga acctgcaaag ccgtggcaca
     4141 ggcaagcgtg cagaagcatg ggcaacaggt ctgaaatacg atgccaataa tatttatctg
     4201 gcaactttct attctgaaac acgtaaaatg acgccaatct ctggcggctt tgccaataag
     4261 acgcagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg
     4321 ggttatgtct tatcgaaagg gaaagatatt gagggcatcg gtgatgaaga tctggtcaat
     4381 tatgtcgatg ttggtgctac gtattatttc aacaaaaata tgtcagcgtt tgttgattat
     4441 aaaatcaacc aactggaaag cgataacaaa ttaaatatta ataatgatga tattgtcgcg
     4501 gttgggatga cgtatcagtt ttaatgaata ttgccggatg tgatgcatcc ggcacatttc
     4561 attcacgccg ttaacttcac cggttcatca cgaaaatcat ctgccggttc cagcttcaga
     4621 ttcagcgtcg ccagcagctc acgcagcttc tcgtgaaatt cacgcagggt gtgctccagt
     4681 cgttcaacca cttcggcatc ttttatctga acactcgtcc agtcgcctgc tttatcgaac
     4741 agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttccagttc catccaccag
     4801 ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg
     4861 aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag tgcggtaaat
     4921 tttttgacta atctgctctt cgggtgtcca ctcggtaacg tcattgcaat ctcctgttgt
     4981 gatgcaactg ttttaccaaa ttggtaaaat ttagcaaatt tttaacacaa acgtttttcg
     5041 atccagtctg tgatttcctg aaggcctttg tcaaaattcc gatacaccgg gttaaatggg
     5101 atctctaata atttaccgtc agcagatgac gacgtgatta agcgtgagtc ctcttccggg
     5161 ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg acgtcccagc
     5221 aatccttgca cttttaatga atagcgattc aactccactc gcaacgcttc atcggaagca
     5281 tcatgcatcc ctaaacgact ggccagaacg tcaagataca tttccggcac ctgttgctgg
     5341 cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac cactttcaga
     5401 cgcggcgatt caaggtatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag
     5461 gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa aacgtgctga
     5521 tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac cgacggcata
     5581 tcaatagtca gcatagcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag
     5641 tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg gccatcgcct
     5701 ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgt
     5761 cgcatcgtgc ccggcagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc
     5821 gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cagcaatgtt gtacaacgta
     5881 gccgcatgca gccagtgtcg accgctgagt tgcggatctt cttccgcaca ggctttttgt
     5941 tgccacacca tcgcctgggt agcccactca taaatccagt tgccgccgcg gtagccaatc
     6001 accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgcgcctgg
     6061 acatcgagga tttcacgggg gtcaatgccg cgccagatcc acatcagacg gttaatcatg
     6121 cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg ttgtgcgccg
     6181 tgactaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg
     6241 gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat
     6301 tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta
     6361 gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt
     6421 attttgcggg atatcaacaa cataatcatc aaccagcgga cgaccggccg gttttgcgaa
     6481 gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt
     6541 atctaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac
     6601 tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg
     6661 aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat
     6721 aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg
     6781 caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc
     6841 tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc
     6901 actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc
     6961 gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc
     7021 tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt
     7081 ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta
     7141 ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag
     7201 agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta
     7261 ccgcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc
     7321 aaaaaaacaa cgacaccgtg catgacttca ctaaagatcc tatccagcct tatattgatg
     7381 gcgaatgggt taaagcacgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct
     7441 ctgcactggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga
     7501 ccatgaccga agaagccggt atggacggtg cttttggctt acagagcaac tggttgcagg
     7561 ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg ggttgtgcgg
     7621 gaggtatcga cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg
     7681 aaaccttcaa gttaacctta aaaggtctga aaggcggtca ctccggcggt gaaatccacg
     7741 ttggcctggg taatgccaac aaactgttgg tgcgcttcct ggcgggtcat gcagaagaac
     7801 tggacctgcg tcttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag
     7861 cctttgcgac cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtggata
     7921 cctatcagga gatcctgaaa aacgaactgg cagagaaaga gaaaaatctg gcattgctgc
     7981 tggactctgt tgcgaacgat aaagctgccc tgattgcgaa atctcgcgat acctttattc
     8041 gtctgctgaa cgccaccccg aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg
     8101 tcgaaacctc cctgaacgtc ggtgtggtga ccatgaccga caataacgta gaaattcact
     8161 gcctgatccg ttctctgatc gacagcggta aagactacgt agtgagcatg ctggattcac
     8221 tgggtaaact ggcaggtgcg aaaacagaag caaaaggcgc atatcctggc tggcagccgg
     8281 acgctaactc tccggtaatg catctggtac gtgaaaccta tcagcgtctg ttcaacaaaa
     8341 cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc
     8401 cggaaatgga catggtgtct atcgggccaa ccatcaccgg tccacactct ccggatgagc
     8461 aagttcatat cgaaagcgtt ggccattact ggacactgct gactgaactg ctgaaagaaa
     8521 ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct tccggcagtt
     8581 tcatccttca ttatccttcg ataaaagcca tccctgtgaa cgttcgtcgc gggttgccac
     8641 gttcaatctg gtgatggaac attcgccgct gcgatttcag cgccgcgcta ttttcctgtt
     8701 gctgttgttc cagcttccag gcaatcagta atcgcgccag ccgtttgtta gcatgctgac
     8761 tgcgctctga ctgaaccttc acgctaatac cggatgacag atgcgtggcg cgtaccgccg
     8821 agtcggtttt attgacatgt tgaccgcccg gccctgacga acgcagcgtc tcataacgga
     8881 ttgcatccga ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga aaccagtttt
     8941 tgcgcccatg atgaggccga tacggactcg gacaaatcca ctgaatagtg ccgcaccagc
     9001 tttcgcttaa agcccatgcg ttatcgccat ccagagaaat cagcgccgaa cgcagtgtgt
     9061 cagagtagcg acccgtttct gtttccagca ccgttaccgc gacgtcctgt cgggtagctt
     9121 ctttaatcag cctgtccagt gcttttttca ctgcgagaca acattcttcc ggcccctgag
     9181 cagaggagag ttgtagcaag atcatccttt tttccctcca ctgtttttga gcgtcagcac
     9241 cgggcgcagt cgcgcgacag gaataattaa cccagccagc accagacatt gcaccacgct
     9301 ttcagccgat ttataggctt gtggcgcttc ttcaaagatg agttgtttat cgcgacaaat
     9361 tacccgactg ccaagttcag tccgcgagag ttgcgtcgcc gtgtatttcg ctgccagacg
     9421 ccctttacac tcggtgcgcc cccatttacg ccccgcccca tgcgccagcg agtggagcgt
     9481 tttttcgttc gcaacaggtt taaccagcca ggagtaatcg ccgcgtgaac cgggaataat
     9541 caccagaccg ttgtcatccg gcgtggcgcc tttacgatgc aaccagccct gttgatcgcc
     9601 gatttggcac gcgctaacaa agttatgcgc cacatccaga accggacttc cggtggcctt
     9661 aacttgttgc attatgcgca aagcgatcag ctggcgatta atacgcgcaa acgccagcgc
     9721 atcgtcatgt tccgcaatat accgtagcgc gtcgtcactg ccttcaggca aaccatgatg
     9781 cgaaaacgag gcaatatgcc gctgtaaaat agactgcccc aatcccctcg agccgctatg
     9841 aaccagcagt tgcagatgct gcgcatccag accagccagt gcaaacagtt ccgcgtcgat
     9901 aatttgctca atctgttgca gttctgcgaa gtggttaccg ccaccgatgg agccaagcga
     9961 gttgcgccac ggatgctgtg caaatgctga cggcaggttt tcctccagcc agctttcttc
    10021 agcaacgtca tccagatctg ataatcgctt ttcaaactta tcggcgttgt atttgcgagc
    10081 gagaatatct gtttgccata gcgccatgcc gcagccgata tcattgccga ccagtgccgg
    10141 gtaaaaacga ccaacagaga agaacgctgc gccaatcggg tagccgcgtc cgggatgtaa
    10201 atctggcatc cccacaacgc gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg
    10261 gatcgctaaa ctctcgatcc acaggtcatc agatgcgatg gtaaataccg catcggataa
    10321 gggacgaata tatttgccca tgtaccattc catttatgaa taaaaatcag gaaatggcag
    10381 acgaatccag ggcaagaaaa aagaattaat cctggaaagg tctgcaaaga gtcattgata
    10441 aaggatttgt catgatttac ctccttgcag tttgtggaat aagaaaacgc gcacatacta
    10501 gacgggcgtt attttcattg caagctggat ttaatgttgc ggtttatatc gcatataaaa
    10561 attagtaaac cacgttcccc ggcattcaac gcgctgctgc ttaactgtct gaaaaccata
    10621 acgttcaaaa aagggttttg cggttatgct ggcgtccacc gtaagttcgg attcagactt
    10681 aatcaatggt tttaacaaag cgctggcaac cccgcgtcgg gtgtattccg gttcaacaaa
    10741 taacatatcg atataatgtc caatgcaggt aataaaacca accggttgtg cattaataac
    10801 tgcaacccgc acttgtgatt tcgcgagttt ctccttccag cgagattcgt caatctgcgc
    10861 ccaggcggca atttgctgtg gagaatagtg ctgactcgcc gtcatcgtaa cagctcgaag
    10921 gaaaatagcg catagttgct gaaaatcacc aggctgatag tttcttattt gtatgttatt
    10981 cataatataa attcaaaaac gcatgcgaaa cgcttctgcc attttcccaa ttttatgcat
    11041 ttggttgtta tctcgagcca gtttatgagg ttcaggtttc agaatggcaa tgagcaacca
    11101 tgcttgctca tcaaacgcgc cctgacaata taccaaatgc gctgcgtcat tcgttctgct
    11161 gaattggcgc aactgtggcg gaaatggatt attcacattt gccagatgaa tatgagcaac
    11221 tcgctcaaat ttgattaatg gccaggtaaa cgagtcgtcg tagagtgcat cacgaccaaa
    11281 tatatctggc aaaacaccgt cacgcttata ggaaataaaa tccgccgtta acgcttcaag
    11341 ttcctctgct gtaagttgca ggcgaataag ttttgttttg aatacccgca tccttattcc
    11401 ttaaagtcat tgaaatcatc atccgtcata tcattaagat attgttcagc gcggcgtgta
    11461 atttcctcta atcttgcagc actcggacgg aagcgcgcct ttatgggctt tttctcctct
    11521 tgttctgcaa gcatgtccat taatgcttca aatgcgctgg cacttaagag atatcctgcg
    11581 gggcgattat tagaaagaac cgcaacaggt tgatcaataa agtatttagc tgggttttta
    11641 cgtaactcag tgatattgac cgatttttca gcgagaattc gatgcataca gtgatctcct
    11701 cataataatg cacaccagaa tatacaaaat agtatacatc ataatcccag caccagttgt
    11761 ctttccattt gcgggtcaag caacgtcaca tgcagcccca ccagtcgcac accgcgcccg
    11821 ccgcggcgtt catcccaggt tttacgcgcg gtggcgatta gatcagattt attcagccgc
    11881 ggccagacgt gttcctgggt ggtttgctga aaatcgtcga actttaattt caccccctgg
    11941 cgggcaatca gcaaatcagg ttttaccttt gccaaacgac gctccagttc agggtaaagc
    12001 cgctcgatga tcgcttcaca ttcagaccag tgatgaatat cttccgccat cgtacgttct
    12061 acaccgacgg attttcgcaa cctttcgctg ttaacgtcgc gctcgtcaat gccctgacta
    12121 cgctcccaca aaatgcggcc aaatttgcca aaacgtttga gcagagtcac cagatcacac
    12181 ttttgcacat caccgcaggt tcgtagcccc atcgcttcca gtttagccgc tgagactttg
    12241 ccgacgccgg gaatttttgc cagcggtaag gtttgcagaa atgccggaac ctcagccgga
    12301 gtaatcacaa actgaccgtt gggtttattc atgtcggagg cgattttggc gagaaacttg
    12361 accggcgcaa cgcccgcaga cgccgtcagt tgcagctcgt tgaagatcgt ctggcggatt
    12421 tcctgggcga tgagggtcgc agaaccgtgg caatggacgc tatcggtgac atcgagatag
    12481 gcttcatcca gtgataacgg ttcaatgcgc gaggtatagc gcgagaaaat ttcgcggata
    12541 tgatttgagg cttctttgta ggcgtcaaag cgccctggaa gcaaggtgag atgcgggcat
    12601 aatttgagcg ccatccctgt cggcatagcg ctacgtacgc caaatttacg cgcgggataa
    12661 ttggcggtgc tgattacccc ccgtcgttcg cggctgccgc caatagcaat agggatatcg
    12721 cgcagggcgg gattgtcgcg catctccacc gctgcgaaaa agcagtccat atccacatga
    12781 atgattttac gcattgctca cctctcaaca ctggtaaagt atacagtgat ttcagggttt
    12841 gagaaatgcg taaagattca gcatttaacg cgctcgctgc gaaagcacct gctgcttatc
    12901 cagcttttgc accagcggct gcaccacttt atccccatgc tgaccaaagt attgatacag
    12961 cgtatcggac gcacttttgg tcagcaccat aatctcaatg cgccggttgc ccgcgctttg
    13021 cggatttttg gcatccagca gcatctggtc cgccattgcg cttacctgca tcactttatt
    13081 ttccggcatt ccggcctctt ccagcacccg acgagccgaa agcgcgcgat cgcccgaaag
    13141 gttccagttg ttgtagatat tgtttttgta ggccatcgca tcggtatgcc cggtaataat
    13201 aattttgtta tcgagcgagt cgaacactgg cgcaagctcc accagcaaag ttttaaagaa
    13261 cggcataatc ttcgcgctgc cgcgttcaaa catattgcgg ttctggtcgt ctttaatcag
    13321 cacgcgtaat ccctggggaa caatctccat ctccagattc gcttccatat gcgcatcacg
    13381 ggcgatggta ttgatactgg tcgccagctc gccaagctcg gtggcggatt tcttcttcaa
    13441 cagcgccgtt ttctcgttga cgtcgcgcgc ttttttctcc gtctcttccg gcaccgccac
    13501 ggtggcaggt tttggcagat gagacgggct gattttattt aacggcgaca acccaccacc
    13561 gttaaaaatc gactgcccgt gcagcgcggc gataatgctt tcacgctcgg atttactgac
    13621 gctgttgaca atccacagcg tcataaacag cgccatcatt gccaaagtaa agtcggcgaa
    13681 cgccaccttc cacgccccgc cgtggtgccc ggcatgattc tttttgatct gccgccggac
    13741 gatggtggtt tttgccccac gctcgcggcg gttgcccgtc tttctcatgc ttattcttgc
    13801 tccagcatcg cattcaccca cgcgtcgagg ttggcgaacg tcggtttgga cgccagatgc
    13861 agcaacttac gcccggcgtc caccgccaac agcgtcggtt tgccgcccgc ctgggccacc
    13921 agcacggtac gcacacactc cagcagcgaa tgttccgccc gcgcctgctg ttccatggcg
    13981 ttggcgagcg ggtccatcag gcagtagcag ataaagacgc ctaaaaacgt tcccaccagc
    14041 gctgccgcca ctttcaggcc aatcagggcg atagagccgt caatcgactg catggtgatg
    14101 atgatgccga gcactgctgc acaaatacca aatcccggca tcgcttcggc agtacgttgc
    14161 agcgagcgcg acggcgttaa cagggactct tccgcggtgt ccagttcctg atcgagaatg
    14221 ccttccagct catgggcgtc gattttaccc atcgccatca ggcgaaagtt atcagcaata
    14281 aaggtcacca gccgcttctg ggttaacacc agcgggtatt tctggaaaat ggtgctctct
    14341 tccggctgct caatatgctg atccaacatc cgcagaccgc cgttttgcac catttcgagc
    14401 aactcataca ggcacattaa tagctgacgc tgaaactccg gcccgagctg ttttttactg
    14461 atcacgcctt taatttgatg ggcaatctct ttcagcacat gtttcggatt cccaataatc
    14521 atcgccccga aaccagcacc caaaataatg atcatttccg ccggttgcca gatagccact
    14581 aattgcccgc ccgcttcaaa ataaccgcca aatacgcagc cgagcaccac cagtaaacct
    14641 aatatttttt gcatgatttc ttcactttaa ttcggataga caaaatcacg aattttctgc
    14701 gcgatctgtt tattcagttg acaaattcgg gcctcggtta gccctaatac cagcgcaatc
    14761 tctttgagat tcatctcatg ctggtaatac atggagagga tcagttgctc tttttcgctt
    14821 aattgtgaaa gcgcgtgttg caacatttgc tgggtgacaa actgatcttc cagttcccgg
    14881 ccagcaagcg gaatgcctgg cccttcgccg ttcagcagtt cgtccaggct ggcgagcgtt
    14941 tccgcccctt ccagttgctg atattcgaga taatcttcat gcgagatccc ttcctcagcc
    15001 agctcctgcc actccggttc gtgccccagt tttttacgcg tttcgcgaat caggtctttc
    15061 atctgatgat atttctgtcg cagctggcgc ggtcgccagt cgagagcgcg cagctcatcc
    15121 aggatcgcac cgcgaatacg gtgcacggca tagcccgcaa aaccttcgtc cggcagccca
    15181 tagcgacgaa tggcgttcaa cagccccatt aacgccgttt gttccatatc ctgacggtca
    15241 ataatgcaat tacactgcgg ggcaagttgc cgcaccactt tacgcaccaa cggtaaatag
    15301 gcctgcaagt agtggctctc ctgctgcgga gttaatacca gcgtggcagc aaattcttct
    15361 gactcgaata catcctgtag catgtttata tccgcctggt gattactgga agaccacttt
    15421 tttcaccagc aaatccttat acggtgcggt cattttgcga cgctgaagat ctttctttaa
    15481 tgcgtcagaa atcagtttgc ggatggcgga tattttcatg ccacgcaaat cttcaaattt
    15541 catttccgtc aggcattcaa ccgcaatact ttgatacagc ggttcctgat gctttatttt
    15601 ttccgcctct ttgtcactgg caaccaccat cactaattcc gccgacaaat aatgatccgc
    15661 gccgttgttg tcatgcagcg tgacaatggt ttccggcagc gtcacaaaca cgcttttgct
    15721 ttcatccagg gtttccaccg cttccgtccg cgcaacggca gtcggttttc cttttccggc
    15781 ataatgatgc cagacacccc atccagctcc ggcccccacc accaacgcca gaacggcgct
    15841 gaccacgccc gccaccacga ttttcttcat attgccttta cactttgatc aaaacggatt
    15901 cgttctgttg aaattgaggc gcatcatcaa gctgtaaggc ggcactcacc tcttcctgct
    15961 gatggcccga ctgttgttgc tgctgcgact gcccttcgga ggaaacctgt acgttcacct
    16021 ccataaaatt ttgcgccgtc agatgctggc ggagatcgtc gctaaactgc tgcaacgcgc
    16081 ggcagacttc actttggttc gcgccgatgt gcaccatcag tttgccgttg tcgagttgga
    16141 cggcgatctc aagcttgcca agcgacgggg gatcgagacg aatggtggag atctgctgtt
    16201 gtttgttgag ctgaaagtgg atctgatctt tcaacagcgt cgtcagtttt tcacccagtt
    16261 cgtccatcga aagcgtggct ggtttggcga caatctcctg atgatcgctg accaactctg
    16321 gcgtcttaac ctgcaacacc gcacgcgcaa taaccggttc gctgtccggc actttttccg
    16381 ttttacgctc gacctgcggg cgcgcggtca cacgggttag ttcaggtttc gggcgagctg
    16441 gcggtgttga gacgcgcggc tgcgtcggcg caatggcatg taaatcttca ctggcgtaag
    16501 tcgccagtct ggcctgctgt tcgggtttcg cctgcggcaa ctgaccgatc agctcctgca
    16561 actgcggcgg caacggcgtg agttcctgct gctgcgtcgg gcgttgcggc gcgttttgca
    16621 ccaccgcttt cgtcaattgc tggataaccg gcgatgttgc gacgtttcgc ggctccggcg
    16681 tgtcctgatg cggcgcggca ggttgcggaa gtaacagcgc cattagcgcc tgcatcgcca
    16741 ccggatcgtt ctcctgcggg cgcgtcgcct gatgctgact ggttttagcg ctatgaacgc
    16801 gctccgccac cgccgccacc gccattttcg gcagggtaaa cgcgggcgcg ttttcaccgc
    16861 tcaccggcgg taaaatatcc gctttcaacg acgcggtttc cgcaagtgtt ccgagcgtgg
    16921 caagcaatgc cggattcatg acttctccga aagtaaatta acaattgagt aggccagcac
    16981 gccttcctga tggcgctgat gctgttccat acgcgccttc aacacttctg cacgctgctc
    17041 gcgtcgttta atcagggcgt taaacgcggc acacagttgc gccttcaccg cacacaactg
    17101 cgtcgcatcg gtttccgccg tcgcataatc gtgaaactgg cgcatcacct gctgatgcag
    17161 ggccggaagg cgtcgccagc gttgcttgtc gagggcttcg ttcatcgcct caaccagcgc
    17221 gaaaagttgt ctgcatttat cttccattgc gttaacccag cttgcccgaa agcccttccc
    17281 agccttcgcg cagattgctt aaaatcagca tcacttcatc gattttctct gccgacagtt
    17341 cgccactggc ttcatacaag cgatacacgc agtggtcata gaggcgagaa agattcacca
    17401 ccagctcgcc accggtttca aactccagcg aactggtgag cgcattgaga atgtcgatgc
    17461 atttattgat gctctgggct ttcttctcga aacgccgccc ttcaatgtgg cttctggcgc
    17521 gttccagctc gtccatcagg ccagaaaaca gcaccagcac cagttcaaga ggcgatgccg
    17581 cagcggtacg cgccgcgaga tcgatctctt tgtattgcga ataaccgtcc tgtacttggt
    17641 acatctttaa ggatattcct tctgaattac gcgaatgccg ccatactggc tttcatggtg
    17701 tacacctcaa ccaacgtatt ggtgtactcc tcaagataac ggtcgtagtt agcgttatag
    17761 gtatcggtaa gctgatcgcc ttcgtcctga attttgctca tctgatcatc aatgttctgc
    17821 tggcgcaggg tgatgatgcc gttgctggag tccgtgtagg tattaatcag gtcgtccatc
    17881 tgcgccacca tgctgttgtc gccaacgaaa atagatgtca gaccgtcagg atttttcgcc
    17941 atttcgtcgc tgaactggtc ggagtcaatc tgtaagtggc cgtgggaatc gagggtgatg
    18001 ccatagtcaa caatcgacac gccgttgtag ctggcatggg cgatatcatc gagctggttt
    18061 gccagtgagc tgagtccagc atcccccgca aagaccccgg cgctactgct gtcatcaccg
    18121 tgagtggtta gcgaatcaac cgtatcaatc aaggtgttat aggcatcgac aaaagtctgt
    18181 actttctcct ggctggcgct ggaatcttcg ctgatgttaa aagtggtgag atcgctgtca
    18241 gaatcgctga cttcggtaaa agtcatggtg acgccgggga tcacatcatc aaacgtgttg
    18301 ctgctgttgg tgatagccgg tccgctggcg ctaccgagat gaataatcgc gtcctgagcg
    18361 gaggaaacat ccgtggcaac gggcgcactg gtgctatcgt tgctggcatc atgccccgtt
    18421 acgctcaccg aaaacgcgcc ctgcgccccg gtgctatcgg aggtgagcat aatcgtggtg
    18481 gtgccgtcgg tttttaccag tgccgccgac acgcccggat tgtcatcgga gtcgttaatc
    18541 gcattcacca gttcgctggc gtcgataaaa ccgtcgccgt caccgttctg atccgccgcc
    18601 gacagatcga tatccatagt gctgtcgccc atcgtcagtt caaaagtacc ggtagcggaa
    18661 aaggcgtcgt cgcccatact gaacgtggtt tgctgcccct gcgccagttg ctcaacgaaa
    18721 aacgagtagc ttcccgcctg cgcctgggaa ttggcggaaa cggtcgccga gtcattattg
    18781 ctgctggcgg caaaagtcac cgggccgtcg gtatcgctgt tcagcgcgtc aacggcgctc
    18841 tggaaatcgc tcaacgccga gctgagcgag tccaggccgc tgctttcagc atccagctcc
    18901 gtttgctttt cctgcaagtt tgccgcctgg gtggcaacat ccgcatacgc gatttcttgc
    18961 gcaatggttc ttgggttgat cattgatgac tccggataac aatgtcaggt tattggcaaa
    19021 aggcgtgcca atatttttta tgtgaataat caatagattg aaaaacaggc ggaaatgacg
    19081 tttccggtct ggcggaaaaa agcctgccgc agaaagcaca acaccgcccg caggcggtgt
    19141 tgttcgtggc cggataaggc gtttacgccg catccggcta tcggtgcaca gtgcctgatg
    19201 caacgctgac gcgtcttatc aggcctacgg gtccgcgcct gtttttatgc gtgtgcccat
    19261 ttgtaggccg gataaggcgt tcacgccgca tccggcaatc ggtgcacaac gcctgatgcg
    19321 acgctgacgc gtcttatcag gcctacgggt ccacgcctgt ttttatgcgt gtgcccattt
    19381 gtaggccgga taaggcgttt acgccgcatc cggctatcgg tgcacagtgc ctgatgcaac
    19441 gctgacgcgt cttatcaggc ctacgggtcc atgcctgttt ttatgcgtgt gcccgtttgt
    19501 aggccggata aggcgttcac gccgcatccg gcaatcggtg cacaacgcct gatgcgacgc
    19561 tgacgcgtct tatcaggcct acgagtccgc acatattttt atgcttgtgc ccgtttgtcg
    19621 gcccgaactt actgcagcag gctggaaacc atgctggaca tgctgttgga ctgtttcagc
    19681 atggtgatac cggtctgcat cagcacctgt tgtttggtca tattggacgc ttccgtcgcg
    19741 taatcggtat ccatgatgtt gccgatagcc acttcggtat tgtcctgcat gctggtcagg
    19801 ttgttggcgg tatcattcag acggttgata gacgcaccca gtttggactg gatctgcgaa
    19861 acgtcatcca tcgcggtaga aatgctggtg atcatctggt tagccgagcc agacgcagtc
    19921 agttcagtac cgccagaaac gcctgcggtg ccagtggtgt ctgcctgatc cgcactaaag
    19981 gagttgctga tagcagaaag gtcagtcacc agcgtgttca gttggcttga aatattcacg
    20041 gtcatggtgt cggaagattc cgcgcccacc tggaaagtta ctgcgctctg gaacagaccg
    20101 tcagtaccgg tgttgctggt gccggaaaca ccgaacaggt tggtgccgcc gtaagtagtg
    20161 ttttgcagca tgtcagacat ctgctggccc agttcgtcgt attcgtcctg catcgcctgc
    20221 aggtcatcgt cgctgtaggt gccgttcgct gcctgagttg acaggtcttt catacgaccc
    20281 agcacgtcag acatttcgtc aaacatgctg tcggcggttt gcagcatcgc ggtggcatca
    20341 ttgatgttgc tcagcgccac gcccataccg ctggactgtg ccgtcagacg gttagcaatt
    20401 tgtttgcctg cggcgtcatc ggcagaagag ttaatccggt tacctgtcgc caggcgttcc
    20461 atagaagtag acagagaaga gctgcttttg ctgattgcat taacggcggc cattgaggcg
    20521 ttattggtgt taatagataa catgatactg ctccttggga ttaatcgttt tcgtttcgat
    20581 accctgtaaa aacgacacct cgggagcaaa cttaaaaccg ccgttaaaaa aatttcactg
    20641 cggtttatct gatgccactt ttagctaaaa aaagcagagt ttaaattcag ataaaaagat
    20701 tattagcaat atctgccaca tggccaggta tcaaaatatg tctttaacaa atgcttgata
    20761 tagttaagat tgtcctcaat taacgcgttt tctcatcaat gtactgatgc caaaatttac
    20821 attgcgttgc aaatagtacc catttggcta ttgtacctgt cctgtcattt cgttacattt
    20881 ccacgccata tagtgattaa gaataatcct attaccataa aatattaatt atcacgcaga
    20941 aaaatcatct tctcagggac gtataagact gtgctttatg caaagcacta tcaggaaaca
    21001 tttatccctt gctaaggtaa actcaactat gagtattatt atcaacaact ggcggatgga
    21061 cccgtcgctt aatgccttaa tacattgcga aacgggtgaa acacgtcgtc tcggcgaata
    21121 ccattttatt ttgctggaaa cattagcaaa aaatgccgat gtggttttat cgcgatctta
    21181 tttatgtgcc gaagtatgga agaaccgtat tgtcggcggt aacagcctgc cgacggcgat
    21241 ccatgcgttg cgcgttgcta tcgatgatga tggcaaacaa cagaacatta ttaaaacaat
    21301 tccgaagaaa gggtacctgt gcaataaaga gtatgtgtct tttccggagt catcgcctgc
    21361 agaagcgcag attattaccg accagataca gaaaacaacg ccagaagaga tatcttcgac
    21421 aacaccgcct gtacctgtta gaaaaaaaca taaaggactg atggggctgg cgttggccgc
    21481 ggctgttata ttggttggct caaccgtggg gtattcacac ttgaaaagta cacctgacgc
    21541 cccgcaactg gtaaaagagt caattaacag tccaagaata aaaatatttc atcttagttc
    21601 tggaaaggaa aataattccg taccgttact gtcacaaatc ctcgcaccag gaaaagacaa
    21661 actcgataat ttattgtcag cgcataatat gacaatgaca acctattata aatatgttcg
    21721 taaccgactg gaaagcgata ttgttctgcg taaccagtgc aacggcagct ggcaattaac
    21781 ctttaatgtg gaaggttggc agaacagcga tattaatagc gcgatgtatc agaatttaga
    21841 gaagttgtta aatactgtgc agaaatgctg acaagtgcca atatatgccg gatgcgacgc
    21901 taaacgcgtc ttatccggcc aaccgataat taccccaata aattcctcac cgtctggctg
    21961 gaaattcgcg cctgaccgtt caccgcctgc aataaaacct gatagttatg attggcgcta
    22021 tccagcacgc cgccgagatt ttccgacgcc tgcacggcgc tttccgtttg cgggaagcgg
    22081 gacatgccgt cgatcaactg ccgcgccttt tcctgctgca ccgccatttg cgcgcgctgc
    22141 tgattaatgt tttctagcat ttgagaaatt cccgccccgc cttgttgcag gctttgcgct
    22201 aagcggtccg cctgtgacgg ttcagcaaag gttttcagcg gcataaacgc cgacttatcg
    22261 ccatcggtgc gcacgcttaa ggtgcgctcg atgtctggcc actgtttttc ggtggtggag
    22321 aagctgatgc catccgcttg ctgatgcagt tgcacaccca cgcggcgcag agcgttggtc
    22381 agccgcgtct gatactgcgc ggcgctgtca tcctcataca gcatcaccgc cgaaagctgg
    22441 gtctgccgcc cgtcagtgac actgaacatg cgcgttcccg gcgtgggatt gtgtaccaga
    22501 ttcgccagat ccagcgagtg gaaggtcacc cgcgcttcgc cctgcaacac tggttgtaac
    22561 tggcggtcca ccgcgccgcc ggaaagcgcg gcgcgtttgt ccagcatctg catcagcgcc
    22621 gtactctgtg cctgcccgcc cttacgctgg ctatgacgat aatcgagcaa ctgctgttcc
    22681 agttgcccga gatagtgatc cgcctgctgc aacgtggtga gctggtcgtt aagctgtacg
    22741 ctgtaacgct gcgggcgagt ggtaatcagc ggcgaggccg gatattcact cgccgtcgcc
    22801 gggagttttt gccgcaccgc ctgagtgggc gcaacggttg acgacgatac cgcagcgcta
    22861 agtgcgccgc cggtggcgag tgatgaagat gtaagtccga cctgcatact gttcctgaaa
    22921 gagttagaga acgctgaaca aattcaactc actcactttc gagtaagtct tgagggtagc
    22981 ttccattgcc acttccagtc cggtaaaggt gatcgacgct gggccgtaat cgagatcctg
    23041 caatgaacca atcaattcat cattagatgt cgagatatcg gtctgtgcat cgctcaacat
    23101 cgacatggtg ttttgtgtct cgccgagcga cgcaatcgag gcgttgaggt cgtccgaggc
    23161 ggtgtctacc acatcgacgg cgttctgaat atcgctctgc acctgcggat ctgccggatc
    23221 gacgtccggg ttttgcagtt cctgcgacaa ggaattaagc gtgttaagca cgtctaaatt
    23281 actgtcgaag aaatcgctcg ccgccacgtt ggtatccact tccacgccgt tcgacaccgt
    23341 agtctggcga tggtcgctgt tgccctgata gctatagctg tcggtgacgc catcgccatc
    23401 atcgtctacc gccacaatcg gcggctgatc gttgatggtg ccgccaaaga cgtaatgccc
    23461 gtcttcatcc tgatagttca gcgccgcgac cattgattcg gtgagcgact caatatcctg
    23521 gccgaggctt gccagagaat cggcggtatt ggtgccattc gccgcttcaa gcaggtcgtc
    23581 gcgtaccgcc agcaggctgt tgttgacgcc atcaagaata gactcctgct ggcttaagcc
    23641 cgccgacgcg gagtcgatat tgctctgata ctgttcaatc gccgactgct ggcggtttaa
    23701 ctgggtgatg cgcgtggcgg cgatcgggtc atccgacggc tgcaaaattt ccttgccggt
    23761 tgccatctgc tcgaccacat gcgccagaga gccggagaga ttattaaaac tttgcgtcat
    23821 cgagacgtag gtttgttggg tggttactcg cattttcgcg ctcccgctta gctgcacatt
    23881 tccagcaccg aatcgaaaat ctcggccccg gcggaaatga ctttcagatt agcttcataa
    23941 atttgttgat aggtgatcag gttcaccgcc tcttcgtcca tgctgacgcc gctgacgctg
    24001 ctctgctggt tttgcgctgc ggaatagaca ttggacgcgg catcgacttc cgtctggttt
    24061 tgctgactgt aaatgccgat attgctgatg attgacgagc acgcctgccc taccgtcacg
    24121 ctgccgaggt tggctatttc cagcggttcg gtggagatat tgatcagcgc ctgcaggttg
    24181 tcgctgttgc cgctttcatc cggtgaactg gaaaacgcca gctcatcggc agtgatatcc
    24241 gggttaacgg tcagtgggcc gtcggcattg ctggcgtcat aaataaacag tggctcgccg
    24301 gggttaccgt tgagatcgta gccctgcgcc agctggttgt tgaccgcatc ggcgaactgc
    24361 gacgccatgc tgttgatggt gtcggtcagc ggcgtcagca catcgttttg ataatcaaac
    24421 agtgcgccta atgaaccgcc agtgtcggtg gtcatcgtcg agatggttcc ggcgaaggtc
    24481 agcgtcatac ttgctgtacc atcggcgttg gtttccagcg cgatggtcga gctttgctgc
    24541 ccgctcacca gcggctgacc gtttttcagg gtgacgttgt agttgccctg gtcgtcaata
    24601 ttgacctgca cgtccatcat tccgctcagt tcttccacca tctgatcgcg ggcgtcgtac
    24661 agcgcggagg cgttatcgcc gttggcttcg gcctgagcga tttgctggtt atagctggcg
    24721 atgccgctgg tcagcgtatt gatttgcgat accatcgcct gctgctgact gatgatttcc
    24781 gtgctttgcg agtcgatgta atccagcgtg ttgtcgatgc gcaaggacag cgccccggct
    24841 tcgctgatca cctgctcgcg cagggcggaa tcatcggggc tggtggtcgc ttcgttaagg
    24901 gcagcgaaaa agttatcgaa gccgccgctc agactgctgt tatcgtcgct cagtaccgct
    24961 tccagttgtg tgagataccc ttgttgagtg ctgtaatagc cgtaatcgct ggcggcatac
    25021 catacctgat taacctgata ctggttggag acgcggcgaa tactgtcgac ctgcacgccg
    25081 ttaccggcgc tgttcggcga accaccgctg gcaccgatgg tgctgatctc cgccacctgg
    25141 cgggtgtagc ccgtggtcat ggcgttagcg gtgttttgcg ccgtgacgtt cagctccacc
    25201 tgcgccgttg atgcgccgct gtagccgatg ttaatcatgt ccatgttggg tccttaccgg
    25261 taaatcgtgc cggttaaaag cggcgtgaag ttgggggttc ttaagttccg taaaccgggt
    25321 gccaaatgcc ggatgcggcg taaacgcctt atccggccta catacgggcg actcgtaggc
    25381 ctgataagac gcgacagcgt cgcatcaggc atcgtgcacc gaacgccgaa gcctcattta
    25441 tgcctgggcg acaactgctt cacaatcatc gccgcgatcc caatcccgtg ctgctgcgcc
    25501 agggtgttac acaactcatc gtcataaaac ccctgcatca tgcgcaccga atcgctgtta
    25561 aacgggttat ctttcgaatc aaacaactcg ttggttttac gcatctcttt cagcatgttg
    25621 cgtaaaaaaa tcgcctcaaa ctgctccgcg gcctgcttaa tatcattggc ctgaaccttc
    25681 ggccccatta acgcatcgct gccgtcaatc ccgccgctgc cgttgatttt catcagatca
    25741 cctccaggtc ggcatccagc gcgcccgcct catgcagcgc ctgtaaaatc gacataatgt
    25801 cgtccggcgt tgcgcctaaa ctgttcagcg cattgaccag cgttttcagg ctgctcgact
    25861 ccggcaaact caccacgcca gggcgcgcat gattcaccgc gatattgctc tgcggcgtta
    25921 ccgccgtgcg tccgcctgca aaggcatttg gctggctgac gttgctggtt tcgttaatcg
    25981 agacggtcaa actgccgtgc gataccgccg ccgcgtgcag cgtaacgcca tcacccatca
    26041 ccaccgtgcc ggtgcgcgag ttgaacacca cgcgggcgcg gattttctcc gcctgcacct
    26101 gcacatcatc cagttgcgac ataaacgcca cccgcgcgcc gggactggta ggcgcacgca
    26161 cggtaacgtt ggtggaactc tgcgcggtgg caatgccgcc aaacgaggcg ttgatcgccg
    26221 ccgcaatgtt gttggcgtct ttaaacgatg ggcgcttcag gttgagggtg atggtgtcgc
    26281 ccatctgaaa atcgctgggg atctcgcgct ctaccgttgc gccgttagga attaacccgg
    26341 cggttggcgt attaaccgtt acgctcgaac cgctggcccc ggtggcattc atcccgccca
    26401 ccaccacgct gccctgcgcc agcgcataaa cttcgccatc cgcgccgtgc aactgggtca
    26461 gcaacaaagt gccgccacga atacttttgg catcgccaat cgacgaaaca gtgacatcaa
    26521 tggtctgccc gcgcgagtac atcggcggca atgtggcgct caccgccacc gcggcgacgt
    26581 ttttcacctt cgggtcgatt ttattcggca actgcacgcc aaactggcgc agcatattgg
    26641 tgatggtctg gttggtgaat ttgacctggt ttttatcacc ggtaccgtcg aggccgacca
    26701 ccaggctgta gccgaccagt tggttttcac gcacaccctg tacgttgacc agcgactcca
    26761 gcgattgcgc cagcgccacg ccaggcagcg ccaagctcac ggcgataact gcggccttta
    26821 tccagttttg cattgcactc tccggcatta aatcgggaac agaggatggt taaacaaccg
    26881 cgtcagccag cctgcggcgt tggcgtcgct taacgcgccg cgtccggcat aggagatgcg
    26941 ggcatcggcg atccgctggg aggaaaccga gttgtcgttc tggatgtcgt cggcgcgcac
    27001 aaggccgctt aaacggatgt actcatcgcc ctggttgagg gtcagccatt tttcgccgcg
    27061 aatttccagg atgccgttgg gctgcaccgc atggacggaa acggtgatct ccccgcgcag
    27121 gctgttctgc tgctgggaag tggcgctgcc gtcaaagtcg cggttggcgt cgatagagcc
    27181 ggaaagctta tctttggtgt gtccaaaaat cgttggcgcg ccgatatcga cggtgttgct
    27241 tttgccgaag ttggttttcg cctgtttgct cgactgggtg gattcttcga ggatcaccgt
    27301 caggatatcg cccacgcggt aagcccgccg gtcggcggtt agcgaccagt tgtagccggt
    27361 ttcaaacacg cccccggcac gcccgtctgc cggaggcggc gcttcggtgg ttggtggcgc
    27421 aaagtatgcg tcgtcttttt tgaccagaat ggcctgcgat tcacacccgc taaggagtgg
    27481 aagcagcgcc accagccaga tataattttt cacgttctgc ctgtttaaat acagtgagtt
    27541 atgtgctctt gtaggtcgga tagtcgcatc cgacatttcg gccttacagc gtctggctga
    27601 tgtactgcaa catatcgtcc gctgctgaca ccattttggc gttcatctcg tacgcgcgtt
    27661 gcaccgtaat catcgccacc atttcgttga cgatatccac gttcgagcct tccagcgcgt
    27721 tatcctgcaa ggtgccaagc ccatcttcgc ccggtacgcc ttcggtcggc tgtccgctgg
    27781 cggcggtttc cagatagagg ttgtcgccct cggcagaaag cccggccggg ttggcgaagt
    27841 tcaccagcgt cagttgccct aactcagtca cgtcgctgtc gccgggtaag atcgccgtca
    27901 ccgtgccatc ctcaccaaac gcgacgttgg tcgcccctgc cggaacgtcg atttccggct
    27961 gcaacggcaa gccctgcgag ttggtcagta cgccgtcagc gttcacctgc aagttgccgt
    28021 cgcgggtata agcgatatcg ccgttggcct tttgcacctg caaaaatccc tgccccataa
    28081 tggcgacatt catcgcgttg tcggtggttt cgacgttgcc ttcggtgaag gttttctgag
    28141 tgccgacaat gcgtacgccg ctaccgaatt gcagcccggt cggcataatg ttgttctgat
    28201 cgagcatcgc gcccggcgct tcctgggtct ggtaaaacag atcctgaaac atcacccggt
    28261 cgcgcttaaa gcctgtggtg ttaacgttgg cgatgttgtt ggcaatcgcg ctcatttcag
    28321 catcctgcgc tgagaggccg gttttgctga tccatagtgc tgcgttcatg gtggtaaatt
    28381 ccgtgttatt ttttggccgc ggttgccgga tgcggcgtga acgccttatc cgacctacga
    28441 tgtgcggtta cgagccgcgc aataagcggt tgcccgcgtc gctgatatct tcggcggttt
    28501 tcatcatctt gatctgcgct tcgaactggc ggttcatggc gatggacgac atcatctcac
    28561 tcaccgccga gacgttgctg ccctcgagaa aaccgccgct gactttgatg ttttcatcgc
    28621 gctgggccgg aacgccgtcg gcagtcacca gcatcccttc aggatttttc gccaggttcg
    28681 cgaccgggat atccaccagc ttcaggcggt cgatatccat cgtcgcggtc acgtcaccgt
    28741 catcgggcgt cacggagagc gtgccgtcgc tgccaaacga agcaatggcg ttgggcggca
    28801 gaatgatcgg cccgttatct cccagcacca gattgccgtc gatagtcagt tgcccctggt
    28861 catcctgctg aatgttgccg ttgcgggtat acacttcgtt gccgttttta tcctgcaccg
    28921 caatgtaacc cgcgccctga atcgccacgt ccagtgggcg ttcggttttc tccgcaacac
    28981 cagtactgtc gttcacgccg ctgacccctt cctgcgccat atagcgcgtg tcgaagccgc
    29041 cgcctttcac cttgtcattg gtcgccattg ccatatcggc gcgaaaaccg ttggtgttga
    29101 cgtttgccag gttgttggcg ctgatctgct gctcgtacag ggtctgagaa gccccgctca
    29161 gggcggtata aatcaagcgg tccattacat agcctggaac agcgcgtcat cgagctgcgt
    29221 gctggtggcg ataactttgg tgttggcctg atagttacgc tgggcggtca tcaggttgac
    29281 taactcgctg gtgatatcga cgttagagct ttcgagcacg cccgacgaca gcgtgccgca
    29341 ggtgccggaa ccgggaacgc caatcaacgg ggtgccggat tcgccggttt gtacccacgc
    29401 ggtgccgctc actgcctcca ggccgttttc attcgggaag gtcgccagca ccacctgccc
    29461 ctgcaacatg cgctcgccat tgctgtaggt agcgtaaact ttgccatcgt catcaacctg
    29521 cacaccgttc tgagtggcag aggcgtagcc gttggcggcg ttggtggtga cagaaaattc
    29581 agagccatat tgcgtacagg aggagtaatc gacggttaaa tcgatgggtg cggcggcgtc
    29641 ggtctgaaac tcaatggtct gcggcgtgtt tggcgaggtc agtttcccgg tgttcgggtc
    29701 gaaggttaac gtcgtcgccg gaacgccggt ctgctgctga ccgtcgaagg tgtactgcac
    29761 ttcccaggtg ttatcgctgg ttttggtgaa gtactggcat accgaaggtt cgttgcctaa
    29821 tgagtcgtat accgtggtgg tgtagctgtc ggtgtacgag ctgctgttgg tcgcgtcgaa
    29881 cggtacggtt gtgcggtcga tagcgtcgtc gctggcatcg aagttggcgg taaaggtcag
    29941 gctgctgctg gcctgcgccg gaatattacc ggttttgatc tggatatcgg tgactgtacc
    30001 ggtttgcagg gtgcccgtgt catcgaccgg atagccctgc aaataagcac ccgaggcgtt
    30061 gacgatatag ccgtttttgt cggtttcaaa cgatccggcg cgggtataag aaatgttgcc
    30121 cgcgctgtcg caggtaacaa aaaagccgtc gtcgttaatc gccagatcca gcgcgttacc
    30181 ggtggatacc agcgaaccgc cgcgcgaaat actttgcgcg gtgcctgcca cgctgacgcc
    30241 catcgcctga cttccggcat acatggctga aaactgggtg gtcatcgact tatagcctac
    30301 cgttccggcg ttggcgatgt tgttactgat cccgtccagc tgttcgttaa cggcattcag
    30361 ccccgttgca gcaatttcat aactcattgt ttcgtcctgt taattcaaat ttttggcagc
    30421 gtgcccgttg ttttgccgga ttcggcgtga acgccttatc cggcctacga acgggtgcaa
    30481 acataaaaat atgcgcgaac tcgtaggcct gataagacgc gttaagcttc gcatcaggca
    30541 tcttgcaccg aacgccggat gcggcgtgaa cgccttatcc ggcctacaaa accatgcagt
    30601 ttcatgtagg cctgataagc gaagcgcatc aggcaatttg gcaccggaca tcacgccaca
    30661 tcactgtcgg catcgggtac gccaaactgg ctaatcatgg tgaacggtac ttcgcccacg
    30721 cctgcaacgt taagcaccgg ggagctgccg tcgagtggga tgcgcacatc ttccacttcg
    30781 ccggagactt caatcggcac ctcttcttcc ccggtgttgg tcaccaccga gacgtcgtaa
    30841 tcgcccggcg ggatgccgta atccgccgga ttaattgaga aatcgacctg gccgggtcca
    30901 acggacgaac tgccttccgg gatcagcgac accgtatagt catcgcctgc ggcatcggta
    30961 aagtggagat cggcggtggt acaggcgtcg tccagcgtgg cgcgaccgtt aatggtctgg
    31021 tcactcacct gcaaggcggt ggtctgcacc ataatgtcgt cgcccaccag gttgccgagc
    31081 gccatcacct gaatgttgct catcagcacc gcgttggtat tcgcctgcac gctcatctct
    31141 tccatcatcg ccacctgcgc cattgacgaa agctggtcga tatattcggt ggcgtcggtc
    31201 gggtcggtcg ggtcctggtt ttggatctca gcgacaaaca gcgtcaggaa agtatcaacg
    31261 ggcgagctat cgctgctgct tgtcgtgtcg ctggccgcca cgttgttgct ttccgccgtg
    31321 gcggtcggct gcggtgattg cgcattcagc gccagggtat tcatcaggct tctcccagtt
    31381 tcagcaaact ttgctgcata cttttcacgt tgttgagcac atcgacattg gtttcgaaat
    31441 cgcgcgaagc cgacatcatg tccgccatct gttccaccac gttgacgtcg ggataccaga
    31501 catccccgtt agcatcggcg agcggcgaat gtggttcata gcgtttcaca gccccgccgg
    31561 tttgcagcac gtcctgcacc tgcacactgg caccgtcaat ggcatgacga tgtgtacccg
    31621 ccagcaaact gtggtgatac accgccgcaa acaccgggct gcgcgcttta tatgcctgcg
    31681 cctcgctgct ggcgggcgca ttggcattcg ccaggttact ggcaacggta ttcagacgta
    31741 gcgtttgcgc cgtcatcgcc gagccggaaa tctgatagat atcagtaaat gacatgctta
    31801 tttcccttca atggcctctc tgatgccgct gatttgcaga ttcagaaagg tcagactgct
    31861 ttgataatcc atactatttt gtgaaaaccg cgcctgttcg ctgttcagtt ccacggtgtt
    31921 gccatcttcc gacggctgca tcggcacacg gtactggaca tccaccgccg cgttatttgc
    31981 ccgttgcatc tccctggcaa aatcaatatc tttagccttg aaatttggcg tatcgacgtt
    32041 cgccagattc gcagtcagta gctcggtcct ctcaagacgt aacttcactg cctgcggatg
    32101 tactcccaat gcctgctgaa aactgatccc catttcctgc gtcactcctg cttcaatccg
    32161 catacgctaa gcaatcgccg tgccattttt taagtgactg aagagaaagg gtttatgagt
    32221 tttcaatttt gcaggcggaa gttttatttc ctcctggcgc tggccttacc gggctacgcc
    32281 gccgtccatc ccgtgcagca cagcgcgcgg gaacaagtca acgcccaggt actgaatgcc
    32341 gccagccaaa aaattgaatc gctggcgcaa cagcggcgct ggcatgacta tcgctacacc
    32401 tttaaggtct acattccctc gcagattgcg accgccgcgc cctgtactaa aacacccggc
    32461 gtcacgctga cttcgcctgg ggacatcgcc ctcaaccgga tgaacttcac cgtcagttgc
    32521 ccacaaagct ggcagatgaa cgtagcggtg cgcccggatg tactggtgcc agtagtaatg
    32581 gcgaagtcac tggttgcccg cgatacgccg ctcaccgcga atgatgtgga actgagaccc
    32641 tacaacgtca gtgcccagcg ccgggaggtg ttgatggaac ccaatgacgc gattggtttc
    32701 agcagtaaac acgcgctgca accgggcagg ccaatcacca aagaagaact gatatctccg
    32761 gtgctggtcg ggcgcgatca accggtgatg attgtttacc agagcgcggg gatcaccgcc
    32821 tcaatgcccg gcgtagcgct gaaaaatggt cgcaaagggg aaatggtgaa ggtgcgtaac
    32881 accagcagcc agcgggtcat tagcgcgatg gtggcggaaa gcggcgtggt gacaacggta
    32941 agcgcggagt aatttgcgct ggcgattaaa gttttgccgc ctgttaaccg ctttaccgga
    33001 gtaacccacc caaataccga gacgaggttg cgatgaaaat caccccgact atgccaggca
    33061 atcacccgac agccacaatg gcaggctcca gtcagccgcg caaaagcacg gcgaccaccg
    33121 ccacgacgct gagcgctgac gacattaccc aggccggatt gcagtctgct cagcaaacgc
    33181 tgaacgacca gcaagagagc gatatcgaca gcgataaagt ggcacaaatg caggcgatgc
    33241 tagcctccgg cagcctgcaa gtggacaccg aacagttagc cagcgacatg ctgagttttt
    33301 tccagaacta agaggccgat gaagtgagta tccggttaca acaggtgaag gcgttgttgc
    33361 agggaattcg tgacgacgac gcactgtacg attccctgcg tgaacttctc cagcgtcagc
    33421 ggatctgcat gattcgccgc gccagcgagg atctgctggc ggtaaacgac gagataacgc
    33481 agcactatga gcagttgcat gggcatagct atcagcggca tagcctgctg aaaatgctcg
    33541 gtgttagcgt aaatcgtgac ggtctggcgc aggtgtttgc ctggcttccg gcggtacaaa
    33601 aagcggcagc acaacagttg tggcaacgcc ttgagcaaaa agcagaacgc tgcaaagcct
    33661 ataacgataa aaacggcgag ctgttaattc gccagtatga atttattcag gcttttttag
    33721 gcagcgaagc ggattttctc tatcagggat aacgacaatt ccttccccca tcgcggaaac
    33781 ggattttccg cctgattctc aatcccttgt ttttgattac aaaaattccc ggcacgctta
    33841 ttgcatattc cgcaaagtaa taaagactgg aagccaccta tgagttttat tgaatgttac
    33901 gaaccggaat acgtgcgcaa ttttatgacg caatatcccg attcgcattt gtacgtccgc
    33961 aaggtgtgga aaaacgagat tcgccagagc ctggcattga ccgatattga aagttgcaaa
    34021 gcggtgctca acgatgccag ggcctttgat ttacagctca cctaccgccc tgtggaagac
    34081 aatgccgccg agctatccgc gcgcgatgcg atagtaaacc aggccatttt gagcaccctt
    34141 acgctccccg atttaacccc ggagctttcc ctgtatgccg tcggaattgt gctctcccgc
    34201 gccagtaaaa tgccgggcag agacggagat attcttgccc gcttgacgac gctgccacag
    34261 gcgctggccg atcattcaca aaaaggcaca ctacaggcac agttcgccca gctgccgccc
    34321 gtgccacagc tggcgcgcca gttgatgacg ctgctcggga gttttacgtt tgactggtcg
    34381 attctaccgg aaagcccacg caaggcctcg ctgccgttgc aggttacctt gctgacgcta
    34441 catgacgcca acagcaaagc cctgttacaa cagcagctta aggtgcagtg gcaaaccacc
    34501 tggcaacagc attttgccac cgcgccgtgg atgatgcgta actggctgat ttaccgcgtt
    34561 taccatgagg tgattggaca gacagacggc gcagattatt gcccgctggt gtgcgatttt
    34621 tatcttatcc gcacgttaat cagtttatgg acgctggatg gttctccgct gcgacaggag
    34681 gatattttcg ccctcttcgc catgtttgaa cgctggcgag ccagtgaaaa tgccgtgctg
    34741 attcgccaac aaattcaggc tgaatgttcc gcagatcccc tgctttatgc gttttcactg
    34801 ctgtcttgct aacgctttct ggccggtttt cccggcctta tcgacttcgt aaactatcat
    34861 gcaaaccctc atcaaaacac tcgccaccgg gcgcgtaaaa ccgtttgtca gcgtcgtcac
    34921 gccaacctgg aaccgcggca cattcctgcc gtacctgctt tatatgtacc gctatcagga
    34981 ttacccggca gaccgccgcg aactcatcat cctcgacgac tccccgcaaa gccatcaaca
    35041 catcatcgac cgcctgacca atggcacgcc gggatccttc aacatccgct acatccatca
    35101 cccggaaaaa ctgccgctgg gcaaaaaacg caatatgctc aacgaactgg cgcgcggcga
    35161 atacatcctc tgcatggacg atgacgacta ctactcgcca gataaaatct cttactccat
    35221 cgccatgatg caaaaacacc gggcgctcat ctccggttcc gaccagatcc ccatctggta
    35281 cagccacatc aaccgcattt tcaaaagccg cagctttggc ccacataaca tcctcaatgg
    35341 caccttttgc tatcaccgta actacctgaa aaagcaccgt tacgacgacg attgtaattt
    35401 aggcgaggag aaaagtttca ccaacaattt cagcgttaac ccgctgcaac tgcccggcga
    35461 gcgcacgatt ctgtgtatct cgcacagcca caacaccttt gataaagact ttatcctcgg
    35521 ggccagcacg cctgtaaacg cggcgcttac ggatatcgtc agcgatccgc tgctacgcaa
    35581 cgcttatctg ggtctgcata acgccactca ttatcaggcg atccagcatc aggcgatcga
    35641 tcagatcgtt ctgctgaatc tggataaacg cccggatcgc ttacagcaga tccgtgaaga
    35701 actgacattg ctgcatattc cgcctgagaa gatcacccgc cttgccgcca gtgaaaatga
    35761 aaacggtcaa cggggccgcc agcaatcaca cttgcaggcg ctgcatctgg cgcaacagca
    35821 cggctggcaa aactatctgc tgctggaaga tgacgcggtt attctcaaac aggagaaaca
    35881 tattcaggtg ctgaacgcgc tgctggcgag cctagcgaaa attccctggc aggtgatgat
    35941 cctcggcggc gaaatttcgc agggaacgat gctaaaaagt ttgcctgggc tggtgcacgc
    36001 gcgggactgc cgcaaagtgt gcgcttatct ggtgaacagc cgctactacc cgcagctggc
    36061 gcagcagatg aacaacgatg agcattcgct ggaagacggc tggcagccgc tgctgcgcac
    36121 cgacaaatgg ctggcctgtt acccgagcct ttgttatcag cgaccgggct ttagtgatat
    36181 cgagaaaaaa gcgactgata acattaacca ctacttcaat aagttacccg tagccaccaa
    36241 gccatctaca ttacctatcg ccgataccat cggctttttt atggaaacct cattccacta
    36301 cgcgctctac cgcccaatca tcactgccct acaggcgcaa ggacaaagct gcacgctggt
    36361 gatcaacgac cgcgtgttta aaccgtttct ggatgaaatg ctgaatacgt taaaaagcat
    36421 cgacgatcca cagctcaaag gtatgcgctt atctgaaatg caggcacacg gtcagcgagt
    36481 aaaatgcctg gtcagcccct attacacgcc tgcgctcagt ggcctttccc ccctgaacat
    36541 tcgcgccatg tacggcttag ccaaagaaac gtggaaccac gccgactgga accgttttta
    36601 tcagcgcatt ttgtgttaca gccactacag tcagcgggcg ctggcacatt tcggcagcgc
    36661 aaaagtggtc ggcaatccgc gcttcgacgc ctggcataac ggcacgtttg accgcgctct
    36721 gccggaaaac atccaacccg attcccgtaa acctacggtg ctctacgccc ccacgtttgg
    36781 cgcattaagc tccctgcccc actgggcaga aaaattgggc cgcttaagcg gcgatgtgaa
    36841 cctgatgtgc aaactgcacc acggcacctg ctcgcgcccg gaagaagccg catcgctggc
    36901 actggcgcgc aggcatttaa aacagcgcac cgactctgcc cgccacacgc tggcgctact
    36961 ggcaaaggcc gattacgtgc tgaccgataa cagcggcttt attttcgatg ctatccacgt
    37021 tgataaacgg gtgatcctgc tcgactttcc gggcatgaca acgttgctcg acggtgagaa
    37081 aagttattcc acgcccgaaa gtgccgacca gcaaattcgc gaaatattgc cggtggctca
    37141 tgatgttgcg gaactgcgct acttattgtc agaggcgttt gactggggtg cggtgcatgc
    37201 gaggcttact gagattcgcc atcactattg cgatgcgttt atggatggca aagcgggaga
    37261 acgagcggcg ttggtgattg tggaggcact agggtaaaac gcgcaagtgc ctgatgcgct
    37321 tcgcttatca ggcctacgcg accttgcaat agtttaaatt tgtgaggatt tgcaggtcgg
    37381 ataaggcgct tacgccgcat ccggcacgaa caacgcactt aactaccctc tgatttcaaa
    37441 ctcatcgctt tatgactggt gaatcggtaa aagtcgtaga ttgtggggga gcgatcttta
    37501 tgaattctga aaaaacctga atcttgctaa acatattgct tatcataaat ataggttaca
    37561 cactataaca attattgcgt aatttaacat tttgttaaaa ttgatagagc ataaacgata
    37621 acatgactta tgataaaaaa gtaacttccg gtacaacaag ccagaaacaa aacaacattg
    37681 tcactcaaaa aatatctcgc ccaaaggaat tatttctatt attttgggtt gatgagtccg
    37741 gtgatagccg acgttctatt tttgaagaag catgtctgac gcgttatt
//
LOCUS       DAZFRE010000042        37652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFRE010000042 DAZFRE010000000
VERSION     DAZFRE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.042"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            345..1715
                     /gene="aroP"
                     /locus_tag="ACHKUI_002450"
     CDS             345..1715
                     /gene="aroP"
                     /locus_tag="ACHKUI_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6598634.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTTDTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1758..2612)
                     /gene="ampE"
                     /locus_tag="ACHKUI_002451"
     CDS             complement(1758..2612)
                     /gene="ampE"
                     /locus_tag="ACHKUI_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6598635.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2609..3160)
                     /gene="ampD"
                     /locus_tag="ACHKUI_002452"
     CDS             complement(2609..3160)
                     /gene="ampD"
                     /locus_tag="ACHKUI_002452"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6598636.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3248..4141
                     /gene="nadC"
                     /locus_tag="ACHKUI_002453"
     CDS             3248..4141
                     /gene="nadC"
                     /locus_tag="ACHKUI_002453"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6598637.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4345..4785
                     /gene="ppdD"
                     /locus_tag="ACHKUI_002454"
     CDS             4345..4785
                     /gene="ppdD"
                     /locus_tag="ACHKUI_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6598638.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4795..6180
                     /gene="gspE"
                     /locus_tag="ACHKUI_002455"
     CDS             4795..6180
                     /gene="gspE"
                     /locus_tag="ACHKUI_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6598639.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6170..7372
                     /gene="hofC"
                     /locus_tag="ACHKUI_002456"
     CDS             6170..7372
                     /gene="hofC"
                     /locus_tag="ACHKUI_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6598640.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQHQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7407..8450)
                     /gene="guaC"
                     /locus_tag="ACHKUI_002457"
     CDS             complement(7407..8450)
                     /gene="guaC"
                     /locus_tag="ACHKUI_002457"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6598641.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8606..8650)
                     /locus_tag="ACHKUI_002458"
     CDS             complement(8606..8650)
                     /locus_tag="ACHKUI_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598642.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8675..9295
                     /gene="coaE"
                     /locus_tag="ACHKUI_002459"
     CDS             8675..9295
                     /gene="coaE"
                     /locus_tag="ACHKUI_002459"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6598643.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQR
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9295..10038
                     /gene="zapD"
                     /locus_tag="ACHKUI_002460"
     CDS             9295..10038
                     /gene="zapD"
                     /locus_tag="ACHKUI_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6598644.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10048..10245
                     /gene="yacG"
                     /locus_tag="ACHKUI_002461"
     CDS             10048..10245
                     /gene="yacG"
                     /locus_tag="ACHKUI_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6598645.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10333..10731)
                     /gene="mutT"
                     /locus_tag="ACHKUI_002462"
     CDS             complement(10333..10731)
                     /gene="mutT"
                     /locus_tag="ACHKUI_002462"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6598646.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLRRVYVE"
     gene            complement(10791..13496)
                     /gene="secA"
                     /locus_tag="ACHKUI_002463"
     CDS             complement(10791..13496)
                     /gene="secA"
                     /locus_tag="ACHKUI_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6598647.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13558..14070)
                     /gene="secM"
                     /locus_tag="ACHKUI_002464"
     CDS             complement(13558..14070)
                     /gene="secM"
                     /locus_tag="ACHKUI_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6598648.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14301..15218)
                     /gene="lpxC"
                     /locus_tag="ACHKUI_002465"
     CDS             complement(14301..15218)
                     /gene="lpxC"
                     /locus_tag="ACHKUI_002465"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6598649.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15319..16470)
                     /gene="ftsZ"
                     /locus_tag="ACHKUI_002466"
     CDS             complement(15319..16470)
                     /gene="ftsZ"
                     /locus_tag="ACHKUI_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6598650.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQAQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16531..17793)
                     /gene="ftsA"
                     /locus_tag="ACHKUI_002467"
     CDS             complement(16531..17793)
                     /gene="ftsA"
                     /locus_tag="ACHKUI_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6598651.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17790..18620)
                     /gene="ftsQ"
                     /locus_tag="ACHKUI_002468"
     CDS             complement(17790..18620)
                     /gene="ftsQ"
                     /locus_tag="ACHKUI_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6598652.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(18622..19542)
                     /gene="ddlB"
                     /locus_tag="ACHKUI_002469"
     CDS             complement(18622..19542)
                     /gene="ddlB"
                     /locus_tag="ACHKUI_002469"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6598653.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19535..21010)
                     /gene="murC"
                     /locus_tag="ACHKUI_002470"
     CDS             complement(19535..21010)
                     /gene="murC"
                     /locus_tag="ACHKUI_002470"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6598654.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21064..22131)
                     /gene="murG"
                     /locus_tag="ACHKUI_002471"
     CDS             complement(21064..22131)
                     /gene="murG"
                     /locus_tag="ACHKUI_002471"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6598655.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22128..23372)
                     /gene="ftsW"
                     /locus_tag="ACHKUI_002472"
     CDS             complement(22128..23372)
                     /gene="ftsW"
                     /locus_tag="ACHKUI_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6598656.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23372..24688)
                     /gene="murD"
                     /locus_tag="ACHKUI_002473"
     CDS             complement(23372..24688)
                     /gene="murD"
                     /locus_tag="ACHKUI_002473"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6598657.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVCLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24691..25773)
                     /gene="mraY"
                     /locus_tag="ACHKUI_002474"
     CDS             complement(24691..25773)
                     /gene="mraY"
                     /locus_tag="ACHKUI_002474"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6598658.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25767..27125)
                     /gene="murF"
                     /locus_tag="ACHKUI_002475"
     CDS             complement(25767..27125)
                     /gene="murF"
                     /locus_tag="ACHKUI_002475"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6598659.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27122..28609)
                     /gene="murE"
                     /locus_tag="ACHKUI_002476"
     CDS             complement(27122..28609)
                     /gene="murE"
                     /locus_tag="ACHKUI_002476"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6598660.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28596..30362)
                     /gene="ftsI"
                     /locus_tag="ACHKUI_002477"
     CDS             complement(28596..30362)
                     /gene="ftsI"
                     /locus_tag="ACHKUI_002477"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6598661.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30378..30743)
                     /gene="ftsL"
                     /locus_tag="ACHKUI_002478"
     CDS             complement(30378..30743)
                     /gene="ftsL"
                     /locus_tag="ACHKUI_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6598662.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30740..31681)
                     /gene="rsmH"
                     /locus_tag="ACHKUI_002479"
     CDS             complement(30740..31681)
                     /gene="rsmH"
                     /locus_tag="ACHKUI_002479"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6598663.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31683..32141)
                     /gene="mraZ"
                     /locus_tag="ACHKUI_002480"
     CDS             complement(31683..32141)
                     /gene="mraZ"
                     /locus_tag="ACHKUI_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6598664.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32743..33747)
                     /gene="cra"
                     /locus_tag="ACHKUI_002481"
     CDS             complement(32743..33747)
                     /gene="cra"
                     /locus_tag="ACHKUI_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6598665.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33829..33915)
                     /locus_tag="ACHKUI_002482"
     CDS             complement(33829..33915)
                     /locus_tag="ACHKUI_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6598666.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33927..34418)
                     /gene="ilvN"
                     /locus_tag="ACHKUI_002483"
     CDS             complement(33927..34418)
                     /gene="ilvN"
                     /locus_tag="ACHKUI_002483"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6598667.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34421..36145)
                     /gene="ilvI"
                     /locus_tag="ACHKUI_002484"
     CDS             complement(34421..36145)
                     /gene="ilvI"
                     /locus_tag="ACHKUI_002484"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6598668.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISCPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSYSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36264..36302
                     /locus_tag="ACHKUI_002485"
                     /pseudo
     CDS             36264..36302
                     /locus_tag="ACHKUI_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36463..37407)
                     /gene="leuO"
                     /locus_tag="ACHKUI_002486"
     CDS             complement(36463..37407)
                     /gene="leuO"
                     /locus_tag="ACHKUI_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6598669.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAEVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9156 a        10465 c         9134 g         8897 t
ORIGIN      
        1 atatgacctt ttgtaagatt attgtgctgg tggctaaggc atggtgttca gcaggcagac
       61 aaatccgaca tatagctagt taaaacagta tgttatcaga cttaaagtat ctattaaatg
      121 tgatgaaaaa cttacaattg cttttttttt atccagaaaa tcaacacatc tacataaagc
      181 aggtgcattc tcgcgcatat accattatct tgtgatagtt gaaagtacta tcatcataat
      241 tcagttacat aaaatgtaaa gaaaccaata caacaaacgg aattacaaaa ttacacacgc
      301 attgctgcgt agttcaaaaa aacaacacaa cacgaggttt cataatggaa ggtcaacagc
      361 acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg
      421 gcgcgatagg gacagggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag
      481 ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctag
      541 gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact
      601 ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg
      661 ccatggcaga gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccaa
      721 cctgggtttc tgccgccgtg ttctttgtgg tgatcaacgc catcaacctg acaaacgtaa
      781 aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttatcgcg gtggtagcga
      841 tgatcatctt cggcgcctgg ctgctattca gcggcaacgg cggcccgcag gcgagcgtta
      901 gcaacctgtg ggatcagggt ggtttcctgc cacacggctt caccggtctg gtgatgatga
      961 tggcgattat catgttctct ttcggtggtc tggaactggt ggggatcacc gcagcagaag
     1021 ctgataaccc ggagcaaagt atcccgaaag caactaacca ggttatctac cgcatcctga
     1081 ttttctatat tggctcgtta gccgttctgc tctcactaat gccgtggacc cgcgttacca
     1141 ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt gtagcgaatg
     1201 cgctgaatat cgtggtactg actgctgcgc tctccgtgta caacagctgc gtatattgca
     1261 acagccgtat gctgttcggt ctggcacaac aagggaacgc gccaaaagcg ctggcgtctg
     1321 tcgataaacg cggcgtaccg gttaacacta ttctggtgtc tgcgctggtt acggcattgt
     1381 gcgtactgat taactacctt gccccagagt ccgctttcgg actgttaatg gcgctggtgg
     1441 tatctgcact ggtcatcaac tgggcgatga tcagtctggc gcatatgaaa ttccgtcgtg
     1501 ccaaacaaga acaaggcgtg gtaactcgct tccctgccct gctttatccg ctgggtaact
     1561 ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg
     1621 cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta
     1681 aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccgtgcag
     1741 ggtgagggca ataatcttta caccaacgcc ccgtaaatcg tcagtagcgc aattaccacc
     1801 acgaccacga acgaggtttt cttcgccatt gaaaccgctg cctttggcgt ctccacctta
     1861 tcgacatgcg gttcacgcgc cagagagaac tgtgccagac gcgttaatac ctgatattgc
     1921 gaagtatgga aatcacccag cgaagcaaac caggccggta acgctttctc accatgaccg
     1981 atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg
     2041 ccatcaatgc cggactgtaa acgatgatgc ggcgtctggt atcgcgccag ccagtattgc
     2101 catgcacgca aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc
     2161 agccagaaca gcggagcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc
     2221 tcacgcaaat actcacgttc atcgcagcct gccgggacgc cgtgaatcat ggtgagttcg
     2281 ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga
     2341 taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc
     2401 agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc
     2461 atcgcaataa tggtcatgcc taatgtgcgc ccgagagaaa aatgtttcac ccgccgaaag
     2521 aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa
     2581 attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agcgcacgaa
     2641 accgtgccca atcaaatgca gggccgggat cggttttccg atccggcgca atatcacaat
     2701 ggcccgtcat gttattagcg atatccggat agcgatcaat cagtgcccgc gtaaccgccg
     2761 caagctgttg atactgcgca tcggtatacg ccagcgtatc ggtgccttcc agctcaatcc
     2821 caatagaaaa atcattgcag cgttcacgcc cctgatactg agagactccc gcatgccagg
     2881 cacgtttatc gaaaggaaca tactggacta tttcaccatc gcggcgaatc aaacagtgag
     2941 cggagacgcg caaatgggcg atctcagcaa agaaaggatg cgcctgcgga tcaatagttc
     3001 cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt
     3061 gcaccaccag cagagtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa
     3121 cgcggcgcgc gccagccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg
     3181 gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag
     3241 ttttaacatg ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg
     3301 cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac
     3361 agtcgatgcc aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc
     3421 cacagtgatc acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaggtgtt
     3481 tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat
     3541 caacgccaat caacccttgt tcgaacttga aggcccatct cgcgtgctgt taacgggcga
     3601 acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg tacgccacta
     3661 tgtcgaatta ctggaaggca ccaacacgca gctgttggat acgcgcaaaa ccttacccgg
     3721 cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg
     3781 gctttctgat gccttcctga tcaaagaaaa ccatattatt gcctccggct cagtgcggca
     3841 ggcggtcgaa aaagcgtcct ggttgcaccc ggatgcgcca gtagaagtcg aagtagagaa
     3901 tctggaagaa cttgatgaag ctctgaaagc aggtgccgat atcatcatgc tggataactt
     3961 cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt
     4021 gtctggcaac gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat
     4081 ctccgttggt gcactaacta aacacgtaca ggcactcgac ctttcaatgc gttttcgcta
     4141 attgctaaca gatttatccg ctccatcgcg ggcggataaa tctccccttc cccaactttc
     4201 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc
     4261 tgctttcccg tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc
     4321 caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg
     4381 atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac
     4441 tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc
     4501 gtagagttgt gcgcgctgga acatggtgga ttaaatacct gcgacggtgg cagcaatggc
     4561 attccctcgc ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg
     4621 tcgctgaccg ggcaagaaag tctcaatgga ctaagcgtcg tcatgacgcc gggttgggat
     4681 aacgcaaacg gcgtcaccgg ctggacgcgt aactgcaata ttcaaagtga cagcgcattg
     4741 cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat
     4801 attccacagc tcactgccct gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa
     4861 gaggtggttc atgttgcggt agtcgatgca ccttcgcatg agctactgga cgcattgcat
     4921 ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac
     4981 gccagtcgca cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca
     5041 gagttgctga ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc
     5101 gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta
     5161 ccggacgttt caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac
     5221 ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga
     5281 aatgccgtct catttcgtat tgcgacctta gcatgtcggg gtggtgaaaa ggtggtatta
     5341 aggttgttac agcaggtgaa ccaggcactg gatgttaaca cgcttggaat gcagccgtca
     5401 caactggtgg actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc
     5461 cctacaggca gcggcaaaac ggtcacgctt tatagtgccc tgcaaatgct gaataccgct
     5521 gacattaata tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag
     5581 acgcaaatcc attcgcgtgc cggactcacc tttcagggcg ttttgcgtgc gttattgcgc
     5641 caggatcctg acgtcatcat gatcggagag atccgcgatg gcgaaacggc agaaattgcc
     5701 attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc tacacactaa ctccacctgc
     5761 gaaacgctgg tacgtttaca gcaaatggga gtcgcccgct ggatgctatc atcggcgctt
     5821 acgctggtaa tagcccagcg tctggtacgc aaactttgcc cgcattgtcg ccggcagcaa
     5881 ggggagccca tccatattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca
     5941 cccggttgtg tacattgcta ccacggtttt tatggtcgca cggccttatt tgaagttctg
     6001 cccataacac cggtcatacg tcagcttatt tccgctaata ccgacgttga atcgctggaa
     6061 acgcacgccc gacaggcggg tatgcgaacg ctttttgaaa acggctgcct ggccgtggag
     6121 caaggcttaa ccacctttga agagttaatc cgcgtattgg ggatgccgca tggcgagtaa
     6181 gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg
     6241 ggcagagagc cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccactaag
     6301 cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat
     6361 tcatcaactg gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggctctgct
     6421 ggcggaacag catcccagta agcagtggca agcgttgctg caatcactgg cgcacgatct
     6481 tgaacagggc gttgcctttt ccaatgcctt attaccctgg tcagaggtat ttccgccgct
     6541 ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga
     6601 actggcgcgt cagcaaaaag cccagcatca gttgaccgac aaagtgaaat cagcgttacg
     6661 ttatcccatt atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt
     6721 tctgccggag tttgccgcta tctataagac ctttaacact ccgctaccgg cgctaacgca
     6781 ggggatcatg acgctggcaa gttttagtgg cgaatggggt tggttgctgg tgctattcgg
     6841 ctttctgctg gcgatagcca ataagctgct gatgcgccac ccaaccaggc ttatcgcacg
     6901 gcagaaattg ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac
     6961 gcagatcttt acgattctga cgctgacaca aagtgcaggc atttcttttt tacagggcgt
     7021 tgagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca
     7081 gcacgatatc agtaacggtc accccatctg gctggcgcta aaaaatgccg gtgagtttag
     7141 cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg atctcatgtt
     7201 agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc
     7261 cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc
     7321 aatgtatctg ccaattttcc atttaggcga tgcgatgagt ggaatgggat aacgctggcg
     7381 tgttgctcca cgccagcgtt ggtatattac aggttgttga agatgcggtt ttcttgttcc
     7441 tgcacacgga taaacgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat
     7501 gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc
     7561 agcggcagct taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga
     7621 cgtttcatcg cagactctga gctcatgccg tagaacaaca taaatttctc gccgttctcc
     7681 tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa
     7741 tcggcaccgc cgccaaaggc tttcgcgaca tcgcccggcg tggtgcagcc gccatcgctg
     7801 acgatcattc cgcccagacc gtgcgcagca tcggcgcatt cgatcaccgc agaaagttgc
     7861 ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact
     7921 ttaacgatat cggcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg
     7981 cctgcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag
     8041 tgttcggaat aaccattcgc cacgtcaata caaacgaaat ttaatgccgg gttcaggtcg
     8101 agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac catcacatgt
     8161 ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt
     8221 ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct
     8281 acggtgtcca tattggcggc gataatcggc acgccggacc agctctgacc tgaatgtttg
     8341 aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg
     8401 cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc
     8461 ctggggttaa tggctataag ggtaagagcg caactccagt gacgttatca tacgcactaa
     8521 tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc
     8581 tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg tgcgctcatt tttaacgttt
     8641 tgataatcat gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg
     8701 aggcattggc agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt
     8761 cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc
     8821 cattgctgat cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc
     8881 cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca
     8941 tccgctgatt cagcaagaga cgcaacacca gatccagcga gctacttccc cctatgtact
     9001 atgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt
     9061 ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgacg atgtaactcg
     9121 cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagctcgcc ttgccgtggc
     9181 agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct
     9241 gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag
     9301 acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag
     9361 tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat
     9421 ttcttccgta atgtcagcga attactggat gttttcgagc gcggcgaagt ccgcactgag
     9481 ctgttgaaag aacttgaccg acagcaacgt aaactccaga cctggattgg cgtgcccggc
     9541 gtggaccaga gccgtattga ggcattaatt cagcagttaa aagcggcggg gagcgtatta
     9601 atctccgcgc cgcgtatcgg gcaatttcta cgtgaagatc gtttgattgc tctggtgcgt
     9661 cagcgactga gcatcccggg cggctgttgc agctttgatt tacctacatt gcacatttgg
     9721 ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac
     9781 ccgcttaccc aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt
     9841 aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc
     9901 ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggtcataa gagccgtttt
     9961 gccattcgtt ttatgccgct ggacagtgaa aacggacagg taccagaacg tctggatttc
    10021 gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc
    10081 tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt
    10141 tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc
    10201 gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattc tatctgatgc
    10261 gacgctggcc tgataaaacg cgccagcgtc gcatcaggca tcatatgcaa atatcggatg
    10321 cagcgtgacg ccttattcga catacacacg cctaagtttc gcaattaccg gttcattggc
    10381 tggcggaaaa tcatcggcat taagaccgac cagcgacatc cactcaccgg gttgcccttc
    10441 tttaccccac ggctcccctt cccagctttc gaccagccag aaccacagag ttatatgcct
    10501 gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac
    10561 ttcttcctga agttcacgca ccaccgcctg ttccggcgtt tcgcccattt caactttacc
    10621 gccgggaaac tccagtttat tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat
    10681 ttcattgttc tcgttgcgaa taatacctac cgcaatttgc agctttttca ttgacttaaa
    10741 cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa ctgttagctt ttattgcagg
    10801 cggccatggc actgcttata ttttttacca gaaccacacg ggcaaggatc gttacgtcct
    10861 actttgcgtt caccggtttg cgccgccagt gcagctgcgg cagcagagtc gtcatcctga
    10921 tggctaagct gctgcatttg cgctaaacgc tcggcttcca tacgacgctg ttgttccagc
    10981 tcctcaacct cttcaggcat acgtacctga actttgctca gcgtgctgat aacttcatat
    11041 tttaacgact ccagcatcgc tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc
    11101 ggatctttct gtgcatagcc acgcaggtgg ataccctgac gcagatagtc catcgcagcc
    11161 aggtgctctt tccacagaga gtcaagcgtt tgcagcatga cgcctttctc gaagtgacgc
    11221 atcatctcag caccaaccac ttcttcttta cgctgataca cttcgatgga ctgcgccaga
    11281 atgcgctcac gcagcgtctc ttcatgcagt tctggttctt tatccagcca ctcggcaatt
    11341 ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca gccccggaat atcccacatt
    11401 tcttccagcg actgtggcgg aatgtaggca tcaatggtcg ctttgaacac atcttcacga
    11461 atgctgttga tggtttcgct cacatcgctg acatccagca gttcgttacg ctgggagtaa
    11521 atggcgcgac gctgatcgtt agccacgtca tcatattcca gcagttgctt acgaatgtcg
    11581 aagttacggc tttcaacttt acgctgggcg ttggcaatcg ctttggtcac ccacgggtgc
    11641 tcaatggctt cgcctggctt catacccagt ttacgcatca tgccggatac tcggtcggaa
    11701 gcaaaaatac gcatcagcgc atcttccatc gacaggtaga aacgggaaga accagcatcc
    11761 ccctgacgac cagaacgacc gcgcagctgg ttatcgatac gacgggattc gtgacgttca
    11821 gtaccgatga tatgcaggcc acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg
    11881 gctttaattt tttcaatttg ctctgcagtc ggattttcca gcgcggcaac ttctgcctgc
    11941 cagctaccac cgagcacaat atcggtacca cgacccgcca tgttggtcgc gatagtcacc
    12001 gcagccggat aacctgcctg agcaacaatc gccgcttcgt tggcatggaa tttggcgttc
    12061 aggacgttgt gcttaatacc ggctttggtc agttcatttg acaccagctc cgatttttcg
    12121 atggagattg tacccaccag caccggctgg cctttcgcag tacgttcttt gatatcttca
    12181 atgatcgcct gaattttttc cgcttcagtc atgtagacca ggtccggcag atctttacga
    12241 atcattggac ggttggtcgg aacaacgacg gtatccagct tgtagatgga gctaaattcg
    12301 aaagcttcgg tatcagcagt accggtcatc cccgccagtt tttcatacag acggaagtag
    12361 ttctggaagg tgatcgacgc cagcgtctgg ttttcgttct ggatctgtac accttctttc
    12421 gcttccacag cctgatgcag gccatcggac caacgacgtc cctgcatggt acgaccggtg
    12481 tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt agtcgacgtc gcgggtaaac
    12541 agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca gcatgatgtt ggccggagag
    12601 tacagagact caccttcatc catgatgcct tctttaacca gcagttcttc aatcagaacc
    12661 agaccacgtt cggtcaggtt cacctggcga gatttttcat ccaccgagaa gtggccttcg
    12721 ccctggaagg tttcggagtc ttctttttcc tgacggatca ggtgtggaat aattttattc
    12781 acgcgtttat acatttccga gctgtcttct gccgggccgg aaatgatcag cggtgtacgc
    12841 gcttcatcga tcaggatgga gtccacttcg tccaccagcg catagtgcag tttacgctgt
    12901 acacgttctt caggactgaa cgccatgttg tcgcgcaggt agtcaaagcc gtattcgttg
    12961 ttcgtaccgt aagtgatgtc agctgcgtaa gcttcacgct ttgccggtgc tggcatgccc
    13021 ggcaggttga taccaactgt caggccaaga aattcaaaca gcggacggtt gttttcggcg
    13081 tcacgttgcg ccaggtagtc gttgacggta actacgtgca cgcctttacc ggttagtgcg
    13141 ttcaggtaag caggcagcgt tgcggtcagg gttttacctt caccggtacg catttcggcg
    13201 atgcagcgtt cgttaagaac cataccgccg agtaactgaa cgtcgaagtg acgcatacca
    13261 aagacgcgct tacttgcctc acgcaccacg gcgaaagctt ccgggatcag attttccagc
    13321 acttcgcctt tttccagacg tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg
    13381 gagagttttt ccatctccgg ttccatggca ttgatgatgt tgaccacttt gcgcatccgg
    13441 cgcagggtgc gatcgttacg actaccgaaa actttagtta ataatttgat tagcataata
    13501 aaatctcaaa cgccccgcgt tgcggagtca ataaaatgaa gtaacggttt attgttgtta
    13561 gctgaggcgt tgagggccag cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct
    13621 gaattttgct tgcggggtaa aatgcgcata atcaatgcga taaccctttt cagacggcgt
    13681 gccttcctgg gtcaacagcg cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag
    13741 aggcaaagat tcttcagcaa cgggcagcgt ttgcggtgcc attgcgaagg aaagatgacg
    13801 gattaccgtg cgaatggcat gttgatgcca gtaatcaacg gaatagttcg aattcgggcg
    13861 gcgcgtgttg gcttccagca aggccaattg accaaagtta actttggctg aaggctcgtg
    13921 gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg gcgttgctga gtgcaggcaa
    13981 acctaaactc gccgcgacca tccctaataa gagatgcggc cagaagtagc gtttaccaaa
    14041 ctgtcgccag cgcgtcagta ttccactcac gttattgcca tcccgatcgt attcaaacgt
    14101 atttagcgca caaatcttat cattaaagcc tgaagtccac agcacgaatg aagataagtt
    14161 gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt tttatagctc ttataaaatt
    14221 caaaaaaaaa cgactggttc acctggccgg agagagtgcc agatttgcca gtcgaatttt
    14281 atacgacagt ataaatgtcg ttatgccagt acggctgaag gcgctttgaa ggccaacggc
    14341 agttctgcgt cgtcctggaa ggtcacatat tcccaggctt cctgtttcgc caggacagcc
    14401 tgcagcagtt tgttattcag tgcatgaccg gatttataag cggtaaatgc accgataata
    14461 ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg
    14521 tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat cgtcaacaac gatggcacaa
    14581 tcgaagctgc cgcccaggca caaaccacgg gactgcagat attcgatatc acgcatgaaa
    14641 ccgaacgtac gcgcacggct gatctggcgc ataaatgcat cagcggagaa gttcatcgca
    14701 tagcgctggt tgctggaatc aatagccgga tggttaaaat cgatggtgaa atccagagaa
    14761 aaaccattgt acggcttaaa ttcagcccac ttatcgccat cttcgacacg aacagtttct
    14821 ttaatgcgaa caaatttctt ggcgcagttc aactcgtcga taccggcgtc aagcagcagg
    14881 tatacaaacg gagcggcgct gccgtccatg atcgggattt ccggcgcgtt aacttcgata
    14941 acaatgttat cgatgcccag gcccgcgaga gcagcattga ggtgctctac ggttgaaatc
    15001 cgcacatcat gctcgttgac cagacacgta cagagcatgg tatcacgcac agatttggca
    15061 tcggccggga aatctaccgg tggattcaag tcggtgcgac gatagatgac cccggtgttg
    15121 gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg tatgtaaacc gacacccgtc
    15181 gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg tattatctcg ccaaattacc
    15241 tatccaaccg aagtgtacta tacattcggc gggccagttt agcacaaaga gcctcgaaac
    15301 ccaaattcca gtcaattctt aatcagcttg cttacgcagg aatgctggga tatccagata
    15361 atccggctct ttcgcagttt gcggcgcatt gtcattcacg actttagcaa ccggcttctg
    15421 ctcctgggtc agcggagcca tcccgtgctg ctggtagcga tccatcactg gctgctgagc
    15481 ctgcttattg gtcaccagag tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac
    15541 aacagttacg cgcagctcgt cattcatatc cgggtcaaga gaagtaccga taaccacggt
    15601 cgcgttgtcg gaagcaaatg cacggatggt gttacctacc gtttcgaact catccagacg
    15661 caggtcgaag cccgctgtga tgtttaccag tacgccgcgt gcgccagaca ggtcgatatc
    15721 ttccagcagc ggagaagaga tagccatttc agcagcttct tccgcacggt cttcaccgct
    15781 cgccacgcca gaacccatca ttgcgtagcc catttcagac attacggtgc gtacgtctgc
    15841 aaagtccacg ttcatcaaac ccggacgagt aatcagttcg gcgatacctt gcacagcgcc
    15901 tttcagtaca tcgttcgctg cgccaaacgc atccagcagg gagataccac ggcccagaac
    15961 tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc acatgcttgg acagttcagt
    16021 gatcccctgc tccgcgaatg ccatacgctt cttgccttca aagttgaaag gcttagtgac
    16081 gacagcaacg gtcaggatac ccaaatcttt tgccacttca gcgacgactg gtgctgcacc
    16141 tgtaccggta ccaccaccca tacccgcagc aataaagacc atgtctgcac cttccagcgc
    16201 cgcacgcagt gcatcgcgat cctcatcagc cgcattgcgg ccaacttctg gattagcgcc
    16261 agctcccagt cctttggtga taccgctacc gatttgaatc gtctgtccaa ccgctgtttt
    16321 acgcagcgct tgtgcatcgg tatttaccgc gaagaattca acaccttcaa tgcgctcacg
    16381 caccatgtgt tcaacagcat taccgccgcc gccgccgacg ccgatgactt taatcaccgc
    16441 gtcattggta agttccattg gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg
    16501 aggccgtaat catcgtcggc ctcataaaaa ttaaaactct tttcgcagcc aactattgag
    16561 tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt tctacttcag cttcaccgtt
    16621 aagatgtgac tctttcccat agtgaagcaa tcccaccgcc gtcgaataat acggctcctg
    16681 agcataatcc gttaaaccgg taatattcag cggcgcgccg atacgcactt gcgtatgaaa
    16741 cacgcgctga gcacaggctg caagaccttc gatctgcgct gcgccaccgg ttaatacaat
    16801 gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc ttttcctgca actgcaatat
    16861 ctcttcgttg accagattga gcagctcggt atagcgcggc tcgatcacct ctgccagggt
    16921 ctgacgttgt agactccgcg gcggacgacc gcctacgctc ggcacttcca cgctctcatc
    16981 ttttccaacg atggagccca gcgcacaacc gtggcgaact ttaatcgctt cggcgtcact
    17041 tggcggtgtg ccaaaggcgt aagcgatatc actggtcacg acattgccag cataaggaat
    17101 taccttcgtg tggcgcaatg ccccaccggt ataaacggcg atatccattg taccaccacc
    17161 gatatcaacg acgcagacac ccagttcacg ttcatcttcc gtcaataccg aataacttga
    17221 tgccagaccg gcaaatatca gttggtcaac tttcagccca caacgttcaa ccgctttgac
    17281 gatgtttttc gccatatcat tgtgacatgt gatcaggtgc acttttgcct gcatccgcac
    17341 gcccgaaagc cctaccggat tcttgatccc ttcctgatag tcaatcgcat actcttgcgg
    17401 gatcacatgc aacacacgat gctcatcgcg cacacgcacc gatttcgcgg tatggacgac
    17461 gttttccaca tcttcttgcg tcacttcttc ttcagaaata ggcaccatac caatttcatt
    17521 ctggcagctg atgtgcttac ctgaaagcgc cagatatacc gaagatatct gacaatctgc
    17581 catcaattct gcctggtcaa tggcgcgttg tacgcacttg accacggatt cgaggtcgtt
    17641 caccccgcct ttatccatac cacgcgacgg gcagctgccc acgccaatga tattgaccat
    17701 accgtcgggc agaacttccc ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc
    17761 tactaccagt tttctgtccg tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt
    17821 tgctgagtag attcccctgg cggcaagggc gcccagccta ctgccgctcc agagtcataa
    17881 cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg cctgctgctg taaaaccgga
    17941 taaagttcta caaagcgagc caaacgtttc atcgtatcgc cccggccaag attgagctta
    18001 atatcgttat tcagcgtcaa ctgccaggaa cgtcgcgcgg tcatcgccgc ttccttcaga
    18061 gtaaatctgt cctttgccag catctgcccc atttcgcgat agccctgcaa cacttcattg
    18121 gcgctgcctt ccgggccata cagcattgga agcacctgct tgctggtgcg atctggcggc
    18181 acgctgaagg tgtttccttc cgcgtctacc atatgttgat cattccaccg cgcaatcggc
    18241 acatattcaa ccagatgaat cttcaattca tcaggccact gctttctgac gctcacctgc
    18301 ttaatccacg gcaggcgttg ttctatttgc gtctggatga tgttgacatc ctgggtcata
    18361 aaggtacctg gctcacccaa tgccaggatc gactgccgga tatcgtcatt acgcgtgtaa
    18421 tggcgttcac cggtcaacac cagctttgag agcggcaggc gttgcgcatc ttccatccag
    18481 cccaacacga cccagccgct caccaacact gtcgttaaaa cggtcagcag gaaaaggatc
    18541 cccgccagac gcgttccatt attgcggcga gaagaaacct cttcttcgct atttcgcgtg
    18601 ttcagagcag cctgcgacat attagtccgc cagttccaga attcgtacta ccaactgcga
    18661 gaagctcata cctgcctgac gtgccgccat cggcaccaga ctgtggctgg tcatacccgg
    18721 tgaggtattg gcttccagca gataaaactg tccatcactg tccagcataa cgtcaatacg
    18781 tccccatcct ttgcaaccta acgtcgtcca tgcttttagc actaatgcct gcaaattggc
    18841 ctcttgtgac gcttccagac ctgcggggca gaaatactgt gtctcatcag agagatactt
    18901 cgcctcataa tcatagaagg ttccggccgg ttgaatacgt attgacggta aaatttcttc
    18961 accgagtatc gcaaccgtga actccggccc acttagccat ttttcaatca atacttcttc
    19021 atcgtgctga aatgccaatc ttaatgcatc ttgtagagca ttttctgcta ctacttttga
    19081 cattcccaca ctggaacctt cgcggctcgg cttaacgata accggcaaac ccagagaaga
    19141 aatttctgct aactgcttat cgttaaggcc tttttcaaac tctgcgcggg ttaacgctac
    19201 ccacggcgcg accggtaaac cggcaccctg ccacagaagt ttgctgcgta gtttatccat
    19261 tgaaagcgca gatgccatca ctccgctgcc ggtataaggc aagcccatca gctcgagcat
    19321 gccctgcagc gtaccatctt caccgccgcg accgtgtagc gcgataaaca ctttctgaaa
    19381 acccatcgac ttcagttgcg tcacgtcgac ttctttcggg tcgacaggat acgcgtcaat
    19441 accgccttca cgcagcccgg ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc
    19501 agcggaggtc ccgcccaaca ggaccgcgat tttatcagtc atgttgttct tcctccggag
    19561 tttgcggctt cagtttgatt tcagctaaag aacgagcaat ttttccgata ttaccagccc
    19621 cctgaacgag aatcaggtcg ttaccggtta ataccggtgc cagcatctcg gctacctgcg
    19681 ccgggtccgg caccagaatg ggatcaattt tcccacgtcc acgaattgtg cgacacagcg
    19741 aacggctgtc cgctcccgga attggcgctt caccagccgg atacacttcc agcatcaaca
    19801 gggtatcaac ctgcgtcagc acattggcga aatcatcata caggtcgcgc gtacgggtaa
    19861 aacggtgcgg ctgaaacagc attaccaggt ttttatccgg ccagcctgcg cgcgccgctt
    19921 taatagtggc gtccacttcc gtcgggtggt ggccgtagtc atcgaccagc attgccgtac
    19981 cgctttttcc attcactggc tctagcggga attcaccgag gaaatcgaaa cggcgccccg
    20041 tcccctggaa gctttccagc gcacgcaaaa tagcctcgtc gtcaattccc tcttccgtag
    20101 caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg acctggcgca ttcagggtga
    20161 cgcgcatcgg ctctttgtcc tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct
    20221 gataatcttc tacacgcacg tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgcc
    20281 ccacacgcgg taacaattcg cggatcaccg gatcatcaac acacatcacc gcacgaccgt
    20341 aaaacggcag gttatgcaga aaattaataa aagtctgttt taaattctca aagtcgccct
    20401 ggtaggtatc catgtggtcg gcttcgatat tggtgacaat cgccaccatc ggttgcagat
    20461 gcaggaacga tgcatcactc tcatctgctt cggcaatcag gtaccgacca tgccccaaac
    20521 gcgcatgaac ccccgccgct tttaccagtc cgccgttaac gaaggttggg tcgagccccg
    20581 cttctgcgta gatgctggaa accatcgcgg tggttgtcgt tttgccgtgt gttccggcaa
    20641 tggcgatgcc atgacgaaaa cgcattaact cagccagcat ttcagcacga cggatcaccg
    20701 gaatacgcgc ttcatgagct gcaacaattt ccgggttatc ggcagaaatc gcgctggaaa
    20761 caacgaccac gctggcatca cgtacgtttt ccgggcgatg gttgaaataa atcgtcgctc
    20821 ccagattcat taactgctgc gtgaccggat ttggcgctaa atcggaacca ctgatctgat
    20881 aaccttcatt ggccagaact tcggcaatac cgcccatacc ggcaccaccg atgccgacaa
    20941 aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt
    21001 gtgtattcat tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta
    21061 caattacgcc cgggcagccc ggctcacttc atttgccact cgctcggtgg catccggaat
    21121 ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt tctcgcgacc acccggccag
    21181 ggtgttggcg acagcatcca cgctaagctg tgactgctcg ataattttgg ctgcgcccgc
    21241 tttttccagc ggtagcgcat tccagtattg ctgacggtct ttatgttgaa acggcacaaa
    21301 caacgccggt agtcctgccg cggcgatttc actcaccgtt aacgcaccgg agcggcaaac
    21361 gacgacatcc gcccacgcat acgccgccgc catatcatca ataaattccg tcactttatg
    21421 ctgcggttgt cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttgccgct
    21481 ctgatgccag atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctggtt
    21541 aagaatgcgt gcgccctggg aaccacccac taccaacaca cgaaccggac cttcacgtcc
    21601 agccaaacgt tgctgcggca acggcagcgc cagcacatcg gtacgcaccg ggttacccac
    21661 cacttccgca ttagggaaag cacctggaaa cgcctgcatc actttggtag caatcttcgc
    21721 cagccattta ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc
    21781 taacgaccac gcggccagac cacctggacc tgatacatag ccgcccatac cgagcaccac
    21841 gtcaggtttg tacgctttca taatcgcccg cgcctgacgc caggcgttga agatacgcag
    21901 cggcgcagct atcagtgctt ttataccttt tccacgcaga ccagagatac gaatgaaatc
    21961 aatttcgatg ccatgttttg gcactaagtc cgcttccata cggtcggcag tccccagcca
    22021 gcgaacttgc caaccctgag ccattagatg gtgcgcaacc gccagccccg ggaatacatg
    22081 tccaccggtt ccgcctgcca tcaccattaa tcgcttccct tgaccactca tcgtgaacct
    22141 cgtacaaacg cctgcgcttt ctccagacgc gtttcataat caatacgcaa cagcatcatg
    22201 atggctgtcg acataatcag taagctcgaa ccaccgtaac tgatcagcgg caatgtcaga
    22261 cctttggtcg gtaacatccc cgccgccgcg cctacgttaa ccagcgcctg gaaactaaac
    22321 cagataccaa tagaacaggc gagaaaaccg gaaaaacggt ggtcaatttc taatgctttg
    22381 cggccaatcg acatcgcgcg aaaagcgacg aagaatacca ttaaaagtgc cagcaccaca
    22441 ccgacatacc ccagttcttc gccgataatg gcgaaaataa agtcagtgtg cgcttccggc
    22501 agatactcca gtttttgtac cgagttacct aaaccttgtc cccacagttc accgcgacca
    22561 aacgccatca acgattgcgt taactgatag ccgctgccaa agggatcttc ccacgggttc
    22621 cagaatgcgg taacacggcg gatacggtac ggttcggcga ggatcagcaa cacaaccgct
    22681 gaaatgccca taccgataat ggcaatgaac tgccacaatt tcgctcccgc caggaacaac
    22741 atcgccagcg tagtcacaaa caacaccacc accgtaccaa ggtctggctg tgccagcagt
    22801 aacactgcca acaccagaat tacgcccatc ggtttcagga agccgcgcag gttattacgt
    22861 acttcgtcgc ctttacgcac cagatagttg gcgatatagc aaaacagcga cagttttgtc
    22921 agctccgcag gctgaatacg cagcaaaccg agatcgatcc aacgcgatgc ccctttaacc
    22981 gagctaccca ctaccagaac gatcatcagc aggatgatag atccgagcag catcgtggca
    23041 ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa tgatcgccag aatgaatgcc
    23101 aggatcagat agacgccatc acgcttcgcg aagaagaacg gatcgttggt taagcgttgc
    23161 cctatgggca ttgacgccga ggtcaccatg ataaagccaa tcgccgcgag gccgaaggtc
    23221 agccacagta aggtgcgatc gtacatgatc aggctgtcgg tatctttttc ccgcgagccc
    23281 atcacccagc cctttagcgc cgtggagatc cagaccagaa tactgaatcc tggcaggcgc
    23341 ggcattttca ggcgagggag agataaacgc atcaacctaa ctccttcgcc agacgggcaa
    23401 actcattgcc tcgttgttca aagttcttga attgatcaag gctggcacag gccggggaga
    23461 gcagaaccat atcgcccggc tgaacacgcg tagcaagcaa gcgcatcgcc tgttccatag
    23521 tttcggtttg ttctgccact tccgggcgta gcgccgccag ctgcgcgccg tcacgaccga
    23581 aacaatacag acatacgtta tcgccattca ggtaacgcgc cagtgggcta aagtccgccg
    23641 atttaccatc gccccccagc aacaaatgca gcgtgccgtc tacgtgcagg ccattcagcg
    23701 ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt aacccagcgc acaccgttat
    23761 gctccagcac aacttcaaag cgatgcggca gaccagtgaa tgtggttaac gctttcaggc
    23821 tgctggcacg cggtaaccct gcggcatctg ccagcgccag cgccgccagc gcattggtgt
    23881 agttatgctg cccggaaagt ttcatttctt tcacgttcag cactttctcg cctttcaccc
    23941 gcagccaggt ttcaccctgc tgatgattca ggtgatagtc gcccatgttg acgccaaagc
    24001 tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa ggcatcatca gcattaacca
    24061 cgcaaacttt tgcgttttcg taaatgcgca gttttgctgc acgatactgt tgtaaaccaa
    24121 acggatagcg atccatatga tcttcagtca cgttcagaat ggtcgctgct accgcctgta
    24181 agctggaggt ggtttccagc tggaagctcg acagttccag cacgtacagt tcgcactcat
    24241 catccaacag catcaacgca ggcaggccaa tattgccacc cacaccaacg ttaacccccg
    24301 ccgctttcgc catttcaccc actagcgtgg tgaccgtgct tttgccgtta gaaccggtaa
    24361 tcgccacaat cggtgcttgt gcttcacgac agaacagctc gatatcgcca acgatttcga
    24421 ttccggcatc agcggcagcg cttaaggatg gatgcgccag tgcaataccg ggactggcga
    24481 caatcagatc tgccgccatc agccattcat cattcagacc gcccgtgtgg cgttctacgg
    24541 cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt atccataacg cgcggcgtca
    24601 caccgcgagc gaggaaaaag tccacgcaag aaagcccggt gaggcccagg ccgataatga
    24661 cgacattttt accctggtaa tcagccatga ttaacgtacc ttcagcgttg ccagaccaat
    24721 caggaccagc atcagcgaaa taatccagaa acgcacaatg acgcgcggtt ccggccagcc
    24781 tttcagttca tagtggtgat gaatcggtgc catgcggaaa atacgttgtc cgcgcagttt
    24841 aaaggagccg acctgcagga tgacggaaag cgtttctacc acgaacacgc cccccataat
    24901 caccagcagg aattcctgac gcagcagtac ggcgataata cctaacgcac cacctaacgc
    24961 cagcgaacct acatcgccca taaagacctg cgccggatag gtgttaaacc acaggaagcc
    25021 cagtcctgcc ccgactatcg cggtacagac aatgaccagt tccccggcgt gtcgtagata
    25081 cggtatgtgt aaatagctgg caaagttcat gttgccggtc gcccacgcca ccagcgcaaa
    25141 accaccggcg acaaatacgg tcggcataat tgccagaccg tcgagaccat cggtcaggtt
    25201 taccgcgttg ccagtaccca caatgacaaa gtaagccagc agaatgtaga acagccccag
    25261 ttgcggcatc acatctttaa agaacggaac caccagctgc gttgcgggcg tgtctttgcc
    25321 ggcaaggtac agcgcgaagg caacacccag tgcaatgacc gacatccaga aatacttcca
    25381 acgggcaatc aggcccttgg tgtctttacg caccactttg cgataatcat caacaaagcc
    25441 aataacacca taacctacca gcaccaccag tacgcaccag acgtacggat tggacgggta
    25501 agcccacagc agcacggaga tcacaatcgc cgtcaggatc attatcccgc ccatggtcgg
    25561 tgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta cgcaccacct gaccaaagga
    25621 aagtttttgc aaatgagcaa tcatacgcgg gcccatccac aatgagatga acagcgcggt
    25681 cagcaggctg acgatggcgc gaaacgtcag ataggaaaag acgttaaagc cggaataata
    25741 tttgaccaaa tgttcggcca gccaaactaa catgtcccat tctcctgtaa agcgcgtact
    25801 acctcttcca tggcagcact acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca
    25861 gcaatcagtg atttaagacg cgcgataagc gcagttttat cggaaaaatg ttcgccaacg
    25921 ccgctggcgg tgctgatagc atggctttgt ttacccacac ttaacacgca gtcaatacca
    25981 gcagcttttg ccgcttcgcc cacctgtaca tggcaggctt cgctttcagc gcccagttcc
    26041 gccatatcgc ccaccaccag cacgcggtag cccggcattt cagccagaac ctgaacagcg
    26101 gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga gcagcaactg gttttctgcc
    26161 agttttatgg ggaacagacg gcctggaaca gctttcagat ttgccagccc cgctttgata
    26221 gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg ctgccagcgc attcgcaata
    26281 ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac ctgtaggggt ttgcagggta
    26341 aattccgtac cgtgcgaggt tacatggata ttggtggcgg tgaaatcgct gttggcggca
    26401 ttaggtgaga aacgccacac tttgcgtgag ccaattacgc tctgccagtt cagccagtcg
    26461 ttgttgtcgg cgttcataat ggcgataccg ttttccggca ggccgctaaa gatttcacct
    26521 ttcgctttcg cgacacccgc aagcgatcca aaaccttcca aatgcgccgc tgccaggttg
    26581 ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc aggcaatttc accctgatgg
    26641 ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg ttaagcgcaa cagcgtcatc
    26701 ggtacaccga tgtcgttatt gagattgcct gccgtataaa gtgtgttgcc gcactggctt
    26761 aaaattgccg ccgtcatctc tttaacggag gttttgccgg aggaacctgt cagagcaacc
    26821 acgcgcgccg gaacttgctg gcgaacccat gcagccagtt caccaaacgc cagacgcgta
    26881 tccttgacga ttaactgcgg cagatcaata tccagcggac ggctaaccag tagtgcgcct
    26941 gcgccgccag ctttcgcctg gtcggcaaaa tcatgagcat cgaaacgttc gcctttcagg
    27001 gcaacaaaca ggcagcccgg cgtcagtttt cgcgtgtcag tggttacagc atcaagggta
    27061 atatctgcac cttgcagttc accgttgaga atatcggtaa gttggctaag ggttacgcta
    27121 atcatgcaat cacccccagc agacgcgcca ccgtgacgcg atcggagtag tccagacgct
    27181 gattgccaac aatctggtaa tcctcatggc ctttgcccgc gaccagtacc acatcattct
    27241 ctttagcctg cataacggcg caagtcaccg cttcagcacg gccttccatc actttggcat
    27301 atccggcatc taacattccc gccagaatat cgttgatgat ggcacgaggc tcttcggtac
    27361 gcgggttatc gtccgtcacc accgccacgt cagcaaactc ttcagcaatt gcgcccatca
    27421 gtggacgttt gcctttatca cgatcgccac cgcagccaaa gacgcaccac agcttgcccg
    27481 cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc atccggcgta tgcgcgtaat
    27541 ccaccaccac cgtcggtttg cctggtgcag tgaacacttc catacgtccg caaaccggtt
    27601 gcagacgcgc ggcggttttc agcaaatcag ccagcggata gccgagtgcc agcagtgtcg
    27661 ccagcgccag cagcaggttg ctgacgttga atgcgcccat caggcgactt tcaatttccc
    27721 catcgcccca actcgagcta aagcgaatgg tcgcaccgct gtcgtgatag ttcacgtcgg
    27781 tcgctttcaa ccagcgtccg tgacagttcg gattgatatg atcttccatt gataccgcaa
    27841 ccgcgtccgg cagttttgcc agccagcggc ggcccacttc atcgtcggcg ttaataatcg
    27901 cctgaccgca atgatgctca gaataaagca gccattttgc agcttcgtag tgttccatat
    27961 caccatgata atcaaggtga tcgcggctta agttggtaaa gaccgacgcc gcaaatttca
    28021 atgccgccac acggtgttgt accagcccgt gggatgaaac ttccattgcg caaaaagtcg
    28081 cgccctgatc caccagcccc gccagctcat gctgaacatc gactgccgaa ccggtggtat
    28141 tttctgtcgg gatcactttc cccagcaggc cgttaccaac ggtgcccatt accgcgctgg
    28201 tttcgccaag cagttggctc cactgtgcca gcagctgggt cgtcgtggtt ttgccgttgg
    28261 tgcccgttac gcctacaaga cgtaaattgt cagagggttc atggtaaaag cggcccgcca
    28321 gtgcagataa acgctcgttg agctggctga gatagatgac cggtacgccg tgcatttcac
    28381 ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat aatggcagcc acaccttgcg
    28441 ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg acctactaca gctacaaaga
    28501 gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg
    28561 cgtctggcac ccacggagca agaaggtcgc gcaaattacg atctgccacc tgtaccctcg
    28621 ccttgattaa tcacaaattc atttttatcg cccgttgtca gcgcatccgg ctcgatgttc
    28681 atggtacgca atacgccgcc catgatggca ccaaagaccg gcgcggaaac ggcgccgccg
    28741 tagtatttac ccgcctgcgg atcgttgata acaacaacca gcgcgaagcg cggctgactc
    28801 gcaggcgcaa cgcctgcggt ataagcaata tatttattga tgtagcgacc gtccggcccg
    28861 acctttttcg cggtaccggt tttaatggcg atacgatagc ctttaatcgc cgccttcacg
    28921 ccgccgccgc ccggtagcgc cacgctttcc atcatatgca ccacggtgcg aacaatggat
    28981 tccgggaaga cacgttcacc gggaaccggg gggtcaactt tggtaatcga cagtgggcga
    29041 taaatgccgt agctgccgat agttgcgtag actcgcgcta actgtaacgg tgttaccatt
    29101 agcccgtagc cgaaagagaa ggtggccctc tctatgtcag accaccgttg tttttgagga
    29161 tataagccac tgcgttctcc gaccaacccc aaattggtcg cttttcccag tccaaaacgt
    29221 gagtaagtat ctactaacgc tgaggacggc atcgctaacg ccagtttgga aacaccgacg
    29281 ttactcgact tctgtaatac cccggtcagg gttaattcgc tgtagcgcgc cacgtctttg
    29341 atttcgtgac cgttaattcg ataaggaacg gtattgagta ccgagttttc ccgcaccacg
    29401 ccacgttgca acgcggtcat caccaccatc ggtttaaccg ttgagcccgg ttcaaacacg
    29461 tcggtgatgg tacggttacg catcgcttct ttcggcgtgc cgctcagatt atttgggttg
    29521 tatgacgggc tgttagccat cgccagcact tcaccggtgt tgacatccac cagcacggcg
    29581 ctaccagatt cagccttgtt aaaggccact gcgttgttca gttcgcgata aaccagcgcc
    29641 tgcaggcgtt catcaatact cagcgtcagg ttgtgcgctg cctggctgtc agtagaagaa
    29701 atatcttcaa ttacgcgacc atagcggtct ttacgcacaa tgcgctcacc cggctgcccg
    29761 gtaagccatt tatcgaaact cttctcgaca ccctcaatcc cttggctatc gacgttagta
    29821 aaaccgatga ggtgagcagt cacttcgccg gatggataat aacggcgaga ttcttcacgc
    29881 agatgaatcc ccggcagttt cagttttttg atgtagtccg ccatgtcagg gtttacctga
    29941 cgcgccagat aaataaagcg ccctttcggg ttggcgttaa tgcgggctga gagctgatcc
    30001 agcggaatat tgagcgcgtt agccagcgcc ttccagcgat caccgacgct gataccgcca
    30061 gcgtcatgca cttctttcgg gtcagcccaa atcgctttaa ccggcacgct caccgctaac
    30121 gggcgaccag aacggtcagt aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga
    30181 gaacgcatgt cgccctcttt caccagcata tccggggaga taacttgtaa ccacgctacg
    30241 cgtccgagca gaaaagccag cgccaggaga atacagccgc ataacaacgc aaaacgccaa
    30301 ctgataaagt tggcatgttc ttcctgacgt tttggtttct gcgttttcgc cgctgctttc
    30361 atgcgtcgcg tttatcctta tttttgcact acgatatttt cttgtgacgg atcaacatgc
    30421 tgcatttgca gcttttccgt ggctatcctt tccacccggc tatggtcgcc gagcgcattc
    30481 tcttcaagga tcaggttgcg ccattcaatg tctaaagcat ctcgctccag caccagttgt
    30541 tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg ttaccacggt caccgccgtc
    30601 aaaataatgc aaatgaacag gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg
    30661 ataacgccag gcaaggcatg gcgctcgtgg cttcccatcg atcctttaac tttgcttaga
    30721 gcttctgtca ctctgctgat catgcgttcg tcctctctgc aatacgcaga actgaactac
    30781 gggcacgagg gttctcagcc acctcttctt cgcccggcat caacttacct agtgctcgca
    30841 actgacggcc ccccagtttt ttgagctgct cttctgtcat cggtaaccct gccggaactt
    30901 gcggaccgcg gctgttttca cgcataaaac gtttcacaat gcggtcttca agtgagtgga
    30961 agctgatgat cgaaagccgc ccacccgggg ccagcacatt gagcgagctt tttagcgcct
    31021 gctctatctc ctccagttca ctgtttaccc aaatgcgcac cgcctggaag gtacgggtcg
    31081 cgggatgttt aaacttatct ttcaccggcg ttgcggcagc cacgacttcc gccagttctt
    31141 tggtgcgggt catcggctgt tcgcggttac gctcgacaat ggcgcgggca atgcgtttgg
    31201 caaaacgctc ttcaccatag gttttcaata cccaggcgat atcggcttct tctgcggttt
    31261 gtagccattc agcggctgac tgcccacggg ttgggtccat acgcatgtcc agcggaccat
    31321 cgcgcataaa ggaaaagccg cgctcagcat catcaagttg cggtgaagag acgccaagat
    31381 cgaggagaat gccgtcgatc ttgccgataa gatcgcgctc ggcaacatat tcgcccagcg
    31441 cggagaaagg tccgtggatg atggagaagc gcggatcatc aatagtcttc gcaacggcga
    31501 tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc ttcgccaagc tgcgagagga
    31561 tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat gtagatgcca tcaggacgga
    31621 tattgaggcc attaacggct tcatccagca gcaccgtagt atgtttatag ttttccatca
    31681 ttttatagag acaagtcctg cagtcgctcc gataagtctc cggtagccaa ctgctctgcg
    31741 tcgatatctt ccttgacctg ttgatgccag gttgtttcat cccacagctc aaacttgttg
    31801 aactgtccaa ccagcatcac ttcttttgtc agcccggcat gttgccgcag tactggcgcg
    31861 attaacaatc gacctgcgcc atccatctga cattcgctgg catgacctaa cagtaggcgc
    31921 tgcacacggc gctcaaccgg gttcatgctc gacagacgcg ataatttttg ctcgataatt
    31981 tcccattcag gcagggggta aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc
    32041 atttgaccgg cagcgttctc aagcagctgt tcccgataac gggtaggcac tgataagcgc
    32101 cctttgctgt cgagattgac taacgttgct ccccggaaca tgccagcctc accccttatt
    32161 cccactttgc cccacaattt cccactgaaa ggagtttacg gagcggagga aaagcttgtc
    32221 aagccagtca caaggcatac cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt
    32281 gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat gaatagctgt aagaaaaaat
    32341 ccctcatcgt catcaccgtt ttaaaatgac tcactatttt ccacagcaaa tataaagtgt
    32401 cagtttgcga cgcgagcggc attttaggac atatcttctc tggttaacag tccctgttgc
    32461 gtctgtgtgg cgcttgatgc cagcaggttt gcgcaaaaac aaagaaaagt gtttgttaaa
    32521 tcggcaattc atctctgcat gtaacaaaat aatgcaaaaa cgcgctgtca atatcattta
    32581 attattgtga atttcagcag gtgagttaat taacgggcgg gtttaaaagc atttatcaat
    32641 aatagccagg aatagtctta tttactttaa ggatattgat gtccattccc gtactcaacg
    32701 cgccagagag aaattcacct ggcgcgtact tttgttcgcg gcttagctac ggctgagcac
    32761 gccgcggcga tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc
    32821 gtccaggctt gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc
    32881 cactgccagc accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat
    32941 tgccaggtca gaaggcagtt tgccgtcacg acgtagcgtg acatccatca ccccttgcaa
    33001 caacgcaaac gacgttgtga acagtgcctg cggcatcgga tgcgtttcca gccatttttc
    33061 gaataactgg gcagctgcct cccgctcata gctgttggca taaaggaaat gcacttcgcg
    33121 aggatcatct ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc
    33181 cggcagcgcg ccaagataaa gtaccgtctc ggcgggaaac ttacgtaact cttccgccag
    33241 catttcggca tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc
    33301 gcggtccagc gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc
    33361 aggtggcaac gacgtcgaaa caataatggc atcaacctga cgctgtaaaa ggtgctcaat
    33421 gcagcgcatt tcgttgtcgg gctgatcttc cgagcaggca atcagcagtt gataaccccg
    33481 ttgccgcgcc tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc
    33541 ggggatcaca agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt
    33601 cgggtggtaa ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac
    33661 acggtattgc ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc
    33721 cagccgagcg atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag
    33781 ccattgtact ggtatgggtt aaattcatta acatctaagc gcagaaaatt aactgcggca
    33841 actgctttta ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg
    33901 ctgcaaattg cgcattgaga tcattctcaa cgcattattt tatcgccgcg tgaaagtccg
    33961 accacaccag agcgagcaac ttccacaatt ttcgccacat cgcgaatcga tgctaaaaat
    34021 gcatcaagct tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg
    34081 ataatttgcc cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg
    34141 gcctgaattt tcaccagcat gatttcccgc tcaacgtgcg cgccctgccc caactcgctc
    34201 acgcgcaaga catcgaccag cttgtgtaat tgcttttcga tttgctcaag cactttttca
    34261 tcgcccacgg tctggatggt catacgcgat aatgtcggat cgtcggttgg cgcaacggtc
    34321 aggctttcaa tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct
    34381 gattcatttt cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc
    34441 ttaaccacat ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcactgc
    34501 catcgacggt aacatcaaca aacaccaagc gattattgcg cacctgttcc agcgcctcgc
    34561 taagtttgct ttccagttct tgcggatgag agatctggat cccgacgtga ccataggctt
    34621 ccgccagacg gacgaagtct ggtaaggact gcatataaga ttgtgaatga cggccggaat
    34681 agatcatgtc ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca
    34741 gtacgggcaa ttcatattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc
    34801 cgtcaccagt gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg
    34861 caggtaaacc aaaacccatc gtgccgaggc cgccagagtt gatccagcga cgcggtttgt
    34921 cgaatggata ataaagcgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag
    34981 cgtctccctt cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac
    35041 tgtaagagtc atatttcagg cactgacgag cgcgccactg ttcaatctgc tgccaccagt
    35101 cgcggatctc atccagcggt tgatggacgg attcttgcga caggagttca agcatttgtt
    35161 cgaggacctg gcgagcatcc cccacaatcg ggatatcagc agtcacggtt ttagaaatgg
    35221 aagtaggatc aatatcgata tgcaaaacag tggcatttgg gcagtacttt gccagattgt
    35281 tcgtcgttcg gtcatcaaaa cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg
    35341 tcatattggc ttcgtaggta ccgtgcattc ccaacatgcc cagtgcctgg cgatgcgttg
    35401 ccggaaacgc ccccagcccc atcaaagagg aaacaacggg cagattcaac gcctccaccg
    35461 cttctttcaa ctgcagatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg
    35521 gtttttttgc cgccaccagc gtttgcagag cgcgcttaat ttgcccttta tgcccggagg
    35581 tcgtgggatt gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg
    35641 ccggatttag aatatctttc ggtaaatcaa cgaccacggg tccaggacga ccacttgccg
    35701 ctagccagaa agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac
    35761 tgtgtttaac caccgggcaa gaaatcccca ccatgtcgca ctcctgaaag gcatcataac
    35821 ctatcaacga agtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag
    35881 cggtggcaat gccagtaatt gcattggtcg cccctggacc cgacgttacc agcacgacgc
    35941 cgacttcccc ggtcgcgcgc gccagaccat cggccatatg caccgccgcc tgctcatggc
    36001 gaactaaaac atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggaccg
    36061 cgcctccagg ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca
    36121 tctcggctcc agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa
    36181 aacacattaa ccgtccgatc ttgcttaagc aattggcaga cagagacata aacacggggt
    36241 aataagaata aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacagaggat
    36301 gaaattgata ataaatcggc tgaatcctac aacttacagc aatttctcta tttttttaat
    36361 taatcctaaa atagagagca caaacattca gaataaaatt ctacattcaa taaaaaatgc
    36421 agaataaacc agacattgat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
    36481 gactaattgc tcttccatcc actgatggcc tttatcacgc ccggcagctt catgccagga
    36541 gagataacag gttctgctgt tttgttttaa cggcagcggt aatacctgta actctaagga
    36601 ttcagcgaac tcttcagcca accaacgcgg cgcaatagcg accagatgcg tttgcgacac
    36661 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
    36721 cgtgtcatac caggtttgac taaatgatgc gaaacgatcg agcgaaacct cagcatgttg
    36781 ttcgttataa acatcatgct tcagtaacgg gcctttaatt gttggatgat ttttgctggc
    36841 taccaacacc atttcatctt taaataatgg cacactggta aattcagggc gatggaagtc
    36901 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
    36961 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
    37021 ctgcgaggtc agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact
    37081 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
    37141 aactgagcca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
    37201 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
    37261 cgacattccc agagcatgag cggcacgggt gatgttttgc tcctgcatca cggcatcgaa
    37321 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc
    37381 tggatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
    37441 ccttattgga atgcagagaa ataatcctcg agaatatgat ttatcatgca tataaaataa
    37501 gaaaaagcaa aatgagtaaa attaggaaaa cataaagata cgggttctga taaaaacaga
    37561 atccactgcc gagcaaagag tcatttacta cagcataata atccataatg tttttgttgc
    37621 gaaaacaatc taatcataac taccacgaat ac
//
LOCUS       DAZFRE010000043        37166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFRE010000043 DAZFRE010000000
VERSION     DAZFRE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.043"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(42..323)
                     /gene="yehE"
                     /locus_tag="ACHKUI_002487"
     CDS             complement(42..323)
                     /gene="yehE"
                     /locus_tag="ACHKUI_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO6598670.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(586..1695)
                     /gene="apbC"
                     /locus_tag="ACHKUI_002488"
     CDS             complement(586..1695)
                     /gene="apbC"
                     /locus_tag="ACHKUI_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6598671.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     DSEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1827..3860
                     /gene="metG"
                     /locus_tag="ACHKUI_002489"
     CDS             1827..3860
                     /gene="metG"
                     /locus_tag="ACHKUI_002489"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6598672.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4001..7789
                     /locus_tag="ACHKUI_002490"
     CDS             4001..7789
                     /locus_tag="ACHKUI_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO6598673.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTNGQNQVKSFAD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGAPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIANIAENRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGCRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWTGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMINPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQILSKLWLWEGKAESYGWGSNSTQEAQFSVLDTITASELINDIEALFE
                     "
     gene            7799..11431
                     /gene="yehI"
                     /locus_tag="ACHKUI_002491"
     CDS             7799..11431
                     /gene="yehI"
                     /locus_tag="ACHKUI_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO6598674.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMATLACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDSKAVKELARYWSQDIFSDREASYMSHENHFGYTACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNSYLSAPLKQVIDSTDNSCLPEILVSPPWRSKKKMTAPRLDLASL
                     ELTPQVYWQLGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILHGWNSPEAQLAEQALRSGHVEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARELTTHILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGLRQFTVRFDENLNPVIYDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFHSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11492..11794
                     /locus_tag="ACHKUI_002492"
     CDS             11492..11794
                     /locus_tag="ACHKUI_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598675.1"
                     /translation="MIVQKELVAIYDYEIPAPEDPFSFRLEIHKFSELFTGSVYRLER
                     FRLHPTFNDADSLINDALIYIRDDFIDERKLRGESHETVIAIFNCELQNIFNQEIE"
     gene            12496..13584
                     /gene="yehL"
                     /locus_tag="ACHKUI_002493"
     CDS             12496..13584
                     /gene="yehL"
                     /locus_tag="ACHKUI_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6598676.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDYEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13595..15874
                     /gene="yehM"
                     /locus_tag="ACHKUI_002494"
     CDS             13595..15874
                     /gene="yehM"
                     /locus_tag="ACHKUI_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO6598677.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDESPDTQDDSQTLLLRATCMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRSDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVINPDTDPILVTLIDTLAGDGFGKLAPSTSQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLSLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEPPNASAETALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            15867..17003
                     /gene="yehP"
                     /locus_tag="ACHKUI_002495"
     CDS             15867..17003
                     /gene="yehP"
                     /locus_tag="ACHKUI_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6598678.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17000..19003
                     /gene="yehQ"
                     /locus_tag="ACHKUI_002496"
     CDS             17000..19003
                     /gene="yehQ"
                     /locus_tag="ACHKUI_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6598679.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDSAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19128..19589
                     /gene="yehR"
                     /locus_tag="ACHKUI_002497"
     CDS             19128..19589
                     /gene="yehR"
                     /locus_tag="ACHKUI_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6598680.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(19630..20100)
                     /gene="yehS"
                     /locus_tag="ACHKUI_002498"
     CDS             complement(19630..20100)
                     /gene="yehS"
                     /locus_tag="ACHKUI_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6598681.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20147..20866)
                     /gene="btsR"
                     /locus_tag="ACHKUI_002499"
                     /gene_synonym="yehT"
     CDS             complement(20147..20866)
                     /gene="btsR"
                     /locus_tag="ACHKUI_002499"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6598682.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20863..22548)
                     /gene="btsS"
                     /locus_tag="ACHKUI_002500"
     CDS             complement(20863..22548)
                     /gene="btsS"
                     /locus_tag="ACHKUI_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6598683.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGGDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            22770..23501
                     /gene="mlrA"
                     /locus_tag="ACHKUI_002501"
     CDS             22770..23501
                     /gene="mlrA"
                     /locus_tag="ACHKUI_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6598684.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            23561..23668
                     /gene="yohO"
                     /locus_tag="ACHKUI_002502"
     CDS             23561..23668
                     /gene="yohO"
                     /locus_tag="ACHKUI_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6598685.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(23649..24380)
                     /gene="yehW"
                     /locus_tag="ACHKUI_002503"
     CDS             complement(23649..24380)
                     /gene="yehW"
                     /locus_tag="ACHKUI_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6598686.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRVR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(24385..25311)
                     /gene="yehX"
                     /locus_tag="ACHKUI_002504"
     CDS             complement(24385..25311)
                     /gene="yehX"
                     /locus_tag="ACHKUI_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6598687.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(25304..26461)
                     /gene="yehY"
                     /locus_tag="ACHKUI_002505"
     CDS             complement(25304..26461)
                     /gene="yehY"
                     /locus_tag="ACHKUI_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6598688.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGTQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(26468..27385)
                     /gene="osmF"
                     /locus_tag="ACHKUI_002506"
     CDS             complement(26468..27385)
                     /gene="osmF"
                     /locus_tag="ACHKUI_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6598689.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(27575..29872)
                     /gene="bglX"
                     /locus_tag="ACHKUI_002507"
     CDS             complement(27575..29872)
                     /gene="bglX"
                     /locus_tag="ACHKUI_002507"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6598690.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30068..31783
                     /gene="dld"
                     /locus_tag="ACHKUI_002508"
     CDS             30068..31783
                     /gene="dld"
                     /locus_tag="ACHKUI_002508"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6598691.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     VLKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVK"
     gene            complement(31822..32754)
                     /gene="pbpG"
                     /locus_tag="ACHKUI_002509"
     CDS             complement(31822..32754)
                     /gene="pbpG"
                     /locus_tag="ACHKUI_002509"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6598692.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTVAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(32928..33515)
                     /gene="yohC"
                     /locus_tag="ACHKUI_002510"
     CDS             complement(32928..33515)
                     /gene="yohC"
                     /locus_tag="ACHKUI_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6598693.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            33685..34263
                     /gene="yohD"
                     /locus_tag="ACHKUI_002511"
     CDS             33685..34263
                     /gene="yohD"
                     /locus_tag="ACHKUI_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6598694.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(34383..35144)
                     /gene="yohF"
                     /locus_tag="ACHKUI_002512"
     CDS             complement(34383..35144)
                     /gene="yohF"
                     /locus_tag="ACHKUI_002512"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6598695.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     RKVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(35197..36633)
                     /gene="mdtQ"
                     /locus_tag="ACHKUI_002513"
     CDS             complement(35197..36633)
                     /gene="mdtQ"
                     /locus_tag="ACHKUI_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO6598696.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTLAVQVDTA
                     LPTALKNGWPDSQWWLEYHDDQLTSLINNALQNAPYMQVAEQRIQLAEAQAKAVATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGLIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            36857..36940
                     /gene="yohP"
                     /locus_tag="ACHKUI_002514"
     CDS             36857..36940
                     /gene="yohP"
                     /locus_tag="ACHKUI_002514"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6598697.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         9215 a         9898 c         9307 g         8746 t
ORIGIN      
        1 gatttaagct ttctaaatcc gattaaaacc agggtggcaa attaatcgta gcgatttaac
       61 acgattcgac gtttgctatc gctatctgca gcaatcactt ccgtcgttac acctttaact
      121 ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta actgaccaca atgctgttga
      181 ggttgaccat taaccatagc catactgcat gttgggggac tgatcctacc attaatcgtt
      241 aatgtggcag tttctgaaga gaacgcggga gaagccaatc cataggcaag aaaaataatt
      301 cctgacagcc agtacttatt cattgaacgt tatccctgta gtaaaggtta tgcctggaaa
      361 gaatgagtgc ataacaaact atagctgtac atccactaca cagccacgaa ggatgataat
      421 gaagcatcgc cggtacgatc aatcgacttt gtagagtttc ggacgaaggt ccgcagaata
      481 ttcgcagcat taaataagtg ttcattaatg cggcaaaaac aaatacaccg catcaatgta
      541 acatctctgc ggtgtatgaa gaataatcag aatggttaat acgcgttaga ccgcgcggaa
      601 ggaaatctcg cctggaatga cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc
      661 caactggcgg tagatagcgg taaattcgct atcaggacgg ctgataaccg ttggcgtgcc
      721 tttatcgaga tcctcacgta aggagatgtg taacggcatc tgccccagca gctgtgtgtg
      781 gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa atcggctcat ggtgaccaca
      841 gttactgcaa atatgcacac tcatattctc tacaataccg agcaccggaa cttcgacttt
      901 ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac
      961 cacgactgca ccggttactg gaatgttttg tgccagtgtc aattgaatgt caccggtacc
     1021 tggcggcata tcaagcacca gatagtcgag atccggccac aaggtttcct gcaacatttg
     1081 cattaacgcc ttgctcgcca ttgggccacg ccacaccatc gcattgtcgt ctgtcaccag
     1141 ataaccaatt gaattagttg ccaggccatg agacataatc ggtgccatat gggtaccgtc
     1201 cggcgaagtt ggacgctgat tttctgcgcc cagcatggta gggattgatg ggccgtagat
     1261 atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc agtgccaacg ccaggtttac
     1321 cgccgtggag gatttaccca cgccaccttt accggaactg accgcgataa tgttcttcac
     1381 gccgttaatt cccggctgat tcttcacgcg tttcagagtg gctatgttat gcgacagttt
     1441 ccagtcgata gctttcgctc cggtgatgcg cagcagttcg gcgctacatt gctcttttaa
     1501 ctcttcgaac gcgctatgcc agacgaatgg cataaccagt tccacatgca gcgtgtcgtc
     1561 catccaggcg acatggtgca acgctttcag cgttgtgagg ttatgcttca gggttgggtg
     1621 ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg atttggcctg
     1681 ggattgttcg ttcatcccga ctcctttttt gtatagataa accatcagct gatagtttac
     1741 ctgaagaata tagagaagta cttacttaac attttcccat ttggtactat ctaagccctt
     1801 ttcactatta agaagtaatg cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt
     1861 gcgcactgcc gtacgctaac ggctcaatcc acctcggcca tatgctggag cacatccagg
     1921 ctgatgtctg ggtccgttac cagcgaatgc gcggccacga ggtcaacttc atctgcgccg
     1981 acgatgccca cggcacgccg atcatgctta aagctcagca gcttggtatc accccggagc
     2041 agatgattgg cgaaatgagt caggaacatc agactgattt cgcaggcttt aacatcagct
     2101 atgacaacta tcactcgacg cacagcgaag agaatcgcca gttgtcagaa cttatctact
     2161 ctcgtctgaa agaaaacggt tttattaaaa accgcaccat ctctcagctg tacgatccgg
     2221 aaaaaggcat gttcctgccg gaccgttttg taaaaggcac ctgcccgaaa tgtaaatccc
     2281 cggatcaata cggcgataac tgcgaagtct gcggcgcgac ctacagcccg actgaactga
     2341 tcgagccgaa atcggtggtt tctggtgcta ccccggtaat gcgtgattct gaacacttct
     2401 tctttgatct gccctctttc agcgaaatgc tgcaggcatg gacccgcagc ggtgcgttgc
     2461 aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc tggcctgcaa cagtgggata
     2521 tctcccgcga cgcgccttat ttcggttttg aaattccgaa cgcgccaggc aaatatttct
     2581 atgtctggct ggacgcaccg attggctata tgggctcttt caagaatctg tgtgacaagc
     2641 gcggcgacag cgtaagcttc gataaatact ggaagaaaga ttccactgcc gagctgtatc
     2701 acttcatcgg taaagatatc gtttacttcc acagcctgtt ctggcctgcc atgctggaag
     2761 gcagcaactt ccgcaagccg accaacctgt ttgttcacgg ctatgtgacg gtgaacggcg
     2821 cgaaaatgtc caagtctcgc ggcaccttta ttaaagccag cacctggctg aatcattttg
     2881 acgccgacag cctgcgctac tactacaccg ccaaactgtc ttcgcgcatt gatgatatcg
     2941 atctcaacct ggaagatttc gttcagcgcg tgaatgccga tatcgtgaac aaagtggtta
     3001 acctggcgtc ccgtaacgct ggctttatca ataagcgttt tgacggtgtg ctggcaagcg
     3061 agctggctga cccgcagttg tacaaaacct tcactgatgc cgctgaagtg attggtgaag
     3121 cgtgggaaag ccgcgaattt ggtaaagcta tccgtgaaat catggcgctg gctgacctgg
     3181 ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc gaaacaggaa ggccgcgatg
     3241 ccgacctgca ggcaatttgc tcgatgggca tcaacctgtt ccgcgtgctg atgacttacc
     3301 tgaaaccggt actgccgaaa ctgaccgagc gcgcagaagc attcctcaat acagaactga
     3361 cctgggatgg tatccagcaa ccgctgttgg gccataaagt gaatccattc aaggctctgt
     3421 ataaccgcat cgatatgaaa caggttgaag cactcgtgga agcatcaaaa gaggaagtaa
     3481 aagccgctgc cgcgccagta actggcccac tggcagatga tccgattcag gaaaccatca
     3541 cctttgacga cttcgccaaa gttgacctgc gcgtggcgtt gattgaaaac gcagagtttg
     3601 tagaaggttc tgacaaactg ctgcgcctga cgctggatct cggcggtgaa aaacgcaatg
     3661 tcttctccgg cattcgttct gcttacccgg atccgcaagc actaattggt cgtcacacca
     3721 ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg tatctccgaa ggcatggtaa
     3781 tggctgctgg tcctggcggg aaagatatct tcctgctaag cccggatgcc ggtgctaaac
     3841 cgggtcatca ggtgaaataa tcccccttca aggcgctgca tcgacagcgc cttttcttta
     3901 taaattccta aagttgttct cttgcgattt tgtctctctc taacccgcat aaatactggt
     3961 agcatctgca ttcaactgga taaaattaca gggatgcaga atgagacact ttatctatca
     4021 ggacgaaaaa tcacataaat tctgggcggt ggagcagcaa ggcaacgagt tacatatcag
     4081 ttggggcaaa gtcggcacta acgggcaaaa ccaggtaaaa agctttgctg atgctgcggc
     4141 ggcggaaaaa gcggaactga agctgattgc ggagaaggtg aagaagggat atgtggagca
     4201 agcgaaggat aattctttgc aaccttccca aacggtaacg gactctctca aggtagcgga
     4261 cttatccacc attattcagg aacaaccctc tttcgtagca gaaacccgtg cggctgacaa
     4321 aaatacagat gctgttttac catggctggc gaaagatatt gctgtcgttt ttccgcccga
     4381 agttgtacac accacgttaa gtcatcgccg ctttcccgga gctcctgttc agcaagcaga
     4441 caaattgacc caattacgtc gcttagcctg tagtgtatcg caacgggata ataaaacagc
     4501 cacatttgac ttcagcgcct gttctttaga atggcaaaac accgtcgcac aggcgatcag
     4561 tcagatcgac ggcctgaaaa caacacagct accatcaccc gtaatggctg tactcacggc
     4621 acttgaaatg aaatgcacaa gatataaaga gcgtgaggat gttatggatc agattattca
     4681 ggaaggcggt ctggaatatg ctactgatgt aataatacat cttcaacaaa tatctattga
     4741 atgggattat gtcaacaata atattgtttt cctgtcgtct ggcatttcac ctgattactt
     4801 gcagcaatat tccagctttg aattacgcct acgtaaacat ttatcactgg cggaagagtc
     4861 tctctggcaa aaatgtgcac aaaaacttat tgccgcaatt ccacatattc cagaatggcg
     4921 gcaaccatta attgctttgt tattacccga aaaaccagaa attgcacatg aaatttccca
     4981 gcgtctactg gggcaaaaga aattaccctc gcttgagtgg ctaaaaatag tggcgactga
     5041 tgagcacatt cttgcctcat tagaaaaata tcacgagcca tatgccattt ttgatgatta
     5101 ctattgtggt gcgatatggt cagccaccgt attacaggag caaggtgtta cagccctgcc
     5161 ccgatttgct ccctatgccg caagtgacta ctgcgccgat gtgttgcgtc atatcaatca
     5221 tccgttcgca ttaacactgc ttatacgtgt agccgggcat actaaacgct gtcacgatcg
     5281 aatgacgaaa gcctgcgctg cgttcccaca cgcagcactg gcagcactgg cggaacttct
     5341 agtgcaaaaa gaagagaata gttggcgcat tatgctaatg acgatgctta tctcgcatcc
     5401 gacactagca gaacaggtca ttccctggct ttcgacaccc gcagttgcag tgctgaaatc
     5461 atgtcagcaa caactgacac agccctcaaa ccatgccagc gccgatctac tgccggccat
     5521 agtagtctcc ccaccatggc tttcgaaaaa gaaaaaatcg ccgattccgg tgctggattt
     5581 agcaccgctc aacctcgaat ccatatgcac aataaccgac acagaagcta aagagtttca
     5641 aactcattgg gactgggaac cgcataagcc aagtgaaggt gctaaaaatt tcctttactc
     5701 gctggggtat cgccgctggg attttgacac atataaatat atcggcgctt ctgactcggc
     5761 aattgacgca tgggaacgtg aggacttcgc tacactcatc caaatgttta aagcccacca
     5821 tgcaccgtat cagggggagt ggcatctcaa ctcacttccc tttttaccta tgcaaaaagc
     5881 gattaagcta tgggaatttc tcagcaaaga gccgcatacc gcgataaaac cagtcatgct
     5941 ttatttgcga ctggcaggta tgagtggttt tctacactcg ttttcacgct accctcaaga
     6001 aggttttgcg gttgctaatt attttgccgc aacggaactg gctcctgccg tcgcccgcgc
     6061 cttcaacaaa ctcaaaacat tacgacaaga cgccagtagc tggctgctga aatatccgga
     6121 acacgccatt accggcctgc tgcctgcagc gctcggcaaa gccagtgaag cacaggacaa
     6181 cgcccgtgct gccttgcgta tgcttaccga aaacggtcat cagccattgc tgcaagaaat
     6241 cgcccggcgt tataaccagc cggaagtaac cgatgcggtg aacgctctac ttgcgctcga
     6301 tcccttagat aatcacccga caaaaatccc cactcttccg accttttatc agccatcgct
     6361 ctggacgcgc ccgttattaa aagcaaacgc ccaatcactg cctgatagcg cactcctcca
     6421 cctcggtgaa atgctccgct tccctcagga agaggcactg tatccgggat tattgcaggt
     6481 gaaagatgcc tgtaccaccg actcactggc tgaatttgcc tgggatctgt ttaccgcctg
     6541 gcagaccgct ggcgcgccgt cgaaagagag ttgggcgttc actgcgttag gcgttctcgg
     6601 taacgatgac accgcccgca aactgacgcc tttgattcgc gcctggcctg gtgaatccca
     6661 gcataaacgc gccaccgtcg ggttggatat tctcgctgct atcggtagtg atatcgccct
     6721 gatgcagctt aacggcatcg cccagaaact gaaattcaaa gcattacagg agcgggcgaa
     6781 agaaaaaatt gccaacattg ccgagaaccg cgaactcacg gtagcggagc ttgaagatcg
     6841 gttagcaccg gatctcggtc tggatgataa cggttcgttg ctgctggatt tcggcccacg
     6901 ccagttcacc gtcagctttg atgaaacctt aaaaccgttt gtgcgtgacg cttccggctg
     6961 tcgtctgaaa gacctgccca aaccaaacaa aagcgatgat gaatcacagg cgaacgatgc
     7021 ggttaaccgc tacaaattgc tgaaaaaaga tgcgcgtacc gtcgccgccc agcaggtagc
     7081 aaggctggaa tccgccatgt gcctgcgtcg ccgctggtca ccggaaaact ttcagctctt
     7141 cctggttgag catccgctgg tccgccactt aacccgccgt ctgatttggg gcgtttatag
     7201 cgccgaaaac cagctactgg cttgctttcg cgtagcggaa gataacagct acagcaccgc
     7261 tgacgatgat cttttcaccc tgccggaagg cgatatctct attggtattc ctcacgttct
     7321 ggaaatatca ccgacggatg ctgccgcctt cgggcagctt tttgccgact acgaactgtt
     7381 accaccattc cgccagcttg atcgtaacag ttacgcgctg acagaagccg agcgcaacgc
     7441 cagcgaactg acacgctgga caggcagaaa atgcccgagt ggtcgggtaa tggggctggc
     7501 gaataaaggc tggataaagg gcaccccgca ggatgctggc tggatcggct ggatgatcaa
     7561 ccccctgggc cgctggtcgt taatcatgga aatcgatgaa ggttttgcgg taggcatgtc
     7621 gccagccgaa ctgagtgccg agcaaatctt aagcaagctg tggctatggg aaggcaaagc
     7681 agaaagctat ggctggggga gtaattcaac acaggaagcg cagttctccg tactcgatac
     7741 catcaccgcc agcgagctaa ttaacgatat tgaagccctg tttgaataag gaaagagcat
     7801 ggacaaggaa ttaccgtggc tggcggataa cgcccaactg gaacttaaat ataaaaaagg
     7861 caaaacacca ctcagccatc gcaattggcc aggcgaaccg gtacctgtta tcactgaaaa
     7921 catcattcag acactgggtg atgaattgct acaaaaagca gagaagaaaa aaaacattgt
     7981 ctggcgttat gaaaactttt cactggagtg gcagtccgcc atcacgcagg ccatcaactt
     8041 gatcggcgaa cacaaaccct caatcacggc ccggacaatg gcgacgctag cctgtatcgc
     8101 gcaaaatgat agccaacagt tgctcgacga aatcgtccaa caagaggggc tggaatatgc
     8161 aactgaggtg gtgattgcac gccagtttat tgtacggtgt tatgagagtg atcctctggt
     8221 agtaacattg caatatcaga acgaggacta tggctatggt tatcgctcag aaacctataa
     8281 cgaattcgat ctccgattgc gtaagcatct ctctctggca gaggaaagct gctggcagcg
     8341 ttgcgccgac aaactcattg ccgcactgcc aggaataacc aaagttcgcc gcccttttat
     8401 tgcgctgaac ctcccggaaa aacccgaaat cgccaacgag ctggtaagcc ttgaatgttc
     8461 gcaaactcat tttcgctcaa aagagtggtt aaaagttgtt gctgatgact ctaaggcggt
     8521 gaaagaactc gcacgttact ggagccagga tatatttagc gatcgagaag ccagctacat
     8581 gtcgcatgaa aaccacttcg gttacacggc ctgcgctgcc cttttgcgcg aacaaggact
     8641 ggcagctgtt ccgcgcctcg cgatgtatgc acataaagaa gattgcggca gtctgctggt
     8701 acaaattaac catccgcaag tcatccgcac cttgctactg gtggctgata aaaacaaacc
     8761 cagcctgcaa cgtgtggcta aatacagtaa aaacttcccc catgcgacgc tcgccgcact
     8821 ggcagaactg ctggcgttaa aagccccccc tgcgcgccct ggttatccca tcatcgaaga
     8881 ccaaaagctg cctgcacagc aaaaagcacg tgatgaatac tggcatacgc tgttacaaac
     8941 gctgatggca tcgcagccac aactggcaga agaggtgatg ccgtgcttaa gtacacaagc
     9001 ccaggcagtg gtgaatagtt atttatcggc accgctaaaa caggttattg atagcaccga
     9061 taatagctgt ctgcctgaaa tcctcgtttc accaccgtgg cgtagtaaga aaaaaatgac
     9121 agctccacgt cttgatttgg catcgctcga attaactccg caagtttact ggcaactagg
     9181 cgaacgagag agacttgccg ccacagagtc tgcccgttat ttcagcacgg gatcttttac
     9241 ggaacgcatg gaacgaaaaa gtggtcgagt tgtactacag gaactgggtt ttggggatga
     9301 tgtatggctg tttttgaatt atatactccc cggaaaactg gatgctgcac gcaattcact
     9361 cattgttcag tggcattact accaggggcg agttgaagag atcctgcatg gctggaactc
     9421 cccggaagca caattagcag aacaggcgct tcgcagcggt cacgtagaag cgttaattaa
     9481 catatgggaa aatgacaact actcacgtta tcgtccggaa aagagtgtct ggaacctgta
     9541 tttattggca cagttgccgc gtgagatggc tttgactttc tggctgcgta tcaatgagaa
     9601 aaagcatctg ttcgcgggtg aggactattt tctcagtatc ctcgggttgg atacattacc
     9661 tggcctgtta ttggcttttt cacatcatcc aaaagaaaca tttccgttaa ttttaaattt
     9721 cggcgcaaca gaactggccc tgcccgttgc ccgcgtctgg cgacgtttcg cggcgcagcg
     9781 tgatctggct cgccagtgga ttttacaatg gccggaacat acggctactg cacttatccc
     9841 tcttgtcttt acaaaaccca gcgataatag cgaagctgca ttacttgccc tgcgtttact
     9901 gtatgaacaa gggcatggcg aattactgca aaccgtggca aaccgctggc agcgtactga
     9961 tgtatggcct gccctggagc aattacttaa actgggtcca atagaaattt acccggcacg
    10021 tattccaaaa gctcctgatt tctggcatcc ggcaatgtgg tccagaccgc gccttatcaa
    10081 gaataatcag cctgttaccg atgacgctct ggaaattatc ggcgaaatgc tgcgctttac
    10141 ccagggcgga cgtttttata gcggactgga acagctgaaa acgttttgcc agccgcaaac
    10201 gctggcagct tttgcctggg atcttttcac cgcgtggcaa caagctggtg cccccgcaaa
    10261 agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg ctcgggagct
    10321 gacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgctg tcagtggact
    10381 gaacatcctt accctgatga ataatgatat ggcgctgata cagctgcacc atatatccca
    10441 acgggcgaaa tcccgctcct tacgtgataa cgcggcggaa tttcttcagg tggtcgcaga
    10501 aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacat tgggccttga
    10561 tgatccgcag gcgttgagtt ttgattttgg tctccggcag tttaccgttc gctttgatga
    10621 aaacctcaac ccggttatct acgatcaaca aaacgttcgc cagaaaagtg ttccccggtt
    10681 gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa aagggctaaa
    10741 aaaagatgcc actcaggtga gcaaaaacct gctcccacgt cttgaagcag ccctacgtat
    10801 cacccgccgc tggtcgcttg cagattttca ttctctgttt gttaatcatc cctttacccg
    10861 cctggttacc cagcgattaa tatggggggt ttatccggca aatgaaccgc gtcgtttact
    10921 caacgccttt cgtgtggccg cagaggggga gttctgcaat gagcaagatg agccaattga
    10981 cctgcctgcc gatgctctga ttggcattgc ccacccgtta gaaatggcag cagaaatgcg
    11041 cagtgaattt gcacagcttt ttgccgatta cgaaattatg ccgccttttc gccagttggc
    11101 gcggcgcacg gtgctgctca cacctgacga gtcagccagt aacagcctga ctcgctggga
    11161 aggtaaatcc gctaccgttg ggcaacttat gggaatgcga tacaaaggct gggagtcagg
    11221 ctatgaggac gcatttgtct atgacttagg tgcgtaccgg ctggtcctta agttttcacc
    11281 cggttttaac cactacaatg ttgatagcaa agcgctaatg agcttccatt ctcttcgggt
    11341 ttaccgtgac aataaatccg tcacttttgc cgaacttgat gtgtttgatt tgagtgaagc
    11401 gttaagcgca cccgacgtta ttttccatta aaacacagcg ggcagtgatg caactgcccg
    11461 ttatcgacac gacttacctc cgggaaaagc gatgatcgta caaaaagagc tggtcgctat
    11521 ttacgattat gagattcctg ctcctgaaga tccgttttcc ttcagacttg agatccataa
    11581 attctctgaa ttatttacag gttccgtcta tcgactggaa cgattccggc tacatccaac
    11641 atttaatgat gccgactcgc taataaatga tgcgttgatt tatataagag atgattttat
    11701 tgatgagcgg aaattacgag gtgaatcaca tgaaacagta atagcaattt ttaattgtga
    11761 actacagaat atattcaacc aagaaataga ataatatact ctagataatt caagtcgcag
    11821 gaaggcgata agcgagtgaa tccccaggag cttactaaag taagtgacag gggtgaacga
    11881 acgcagtcgc agcacatgca acttgaagta tggcgagtaa agctttatta aagggggaaa
    11941 cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
    12001 aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgtg
    12061 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
    12121 gatactggtt tacgtatgtt ggtacgaaac gttgctctcc ggatcagggc accgcctgcg
    12181 gcaccacccg tccagcagcc aggacttaaa attggtccga tccggcacaa cctcccaacg
    12241 ggcaggatta taaatctcat tcactgaatt gcaatggagt gtggatatgg ttataattta
    12301 ttcgttggta cgaaatgttg cacaatcagc aagttagcgt cattgtagta aataaaagaa
    12361 cctgcctcac cagcaggttt ttttatttac tgtgatctgc tttccagata tttttcgctc
    12421 aaacaactaa tgcggcaaac atttattgcg cgtaaaatat cgtttatttc attaatacat
    12481 ttcagggatg aatatatgtc accgcagaat aatcatctgc agcgtccgcc tgccgctgtg
    12541 ttatacgccg atgaactggc aaaattaaaa caaaatgatc acgcaccttg cccgcccggt
    12601 tggcagttaa gtttgcctgc ggcccgcgct tttatccttg gcgatagcgc gcaaaatatt
    12661 agccgtaaag tcgtgatcag tccctccgct gtcgaacgca tgttagtcac tctcgctacc
    12721 gggcgcggtt tgatgttggt gggggaacct ggcaccgcaa aatcgctcct gtctgaatta
    12781 ctggcaaccg ctattagcgg cgatgccggg ttaaccatcc agggcggcgc atctaccacc
    12841 gaagatcaaa tcaagtacgg ctggaactac gccctgctta tcaaccatgg tccgtcaacg
    12901 gaagcgctgg tccctgcccc gctttaccag ggaatgcgcg acggcaaaat tgtccgtttt
    12961 gaagagataa cgcgtacacc gctggaagta caagactgtc tgttaggtat gctttctgac
    13021 agggtaatga cggtgccgga actcactggt gaagccagcc agctctatgc acgggaaggc
    13081 tttaatatta ttgccactgc caatacccgc gatcgcggcg tcaatgaaat gagtgctgcg
    13141 ctaaagcgtc gtttcgattt tgaaactgtc tttccgatta tggatttcgc ccaggagctg
    13201 gagcttgtcg ccagtgcttc ggcgcgtttg ctggcccata gcggcattcc gcataaagtg
    13261 cctgacgcgg tactggagtt actggtgcgc acctttcgcg atctgcgtgc caacggcgaa
    13321 aagaaaacgt caatggatac actaacagcg attatgtcca ccgctgaagc cgtgaacgtg
    13381 gcccacgcgg tgggcgtccg cgcctggttt ttagcgaatc gcgcaggtga gccagccgat
    13441 ctggtggact gtattgccgg aaccatcgtc aaagattacg aagaagatcg cgcgcgcctg
    13501 cgccgttact tcgaacaacg tgttgctaca cataaagaag ctcactggca ggcttattat
    13561 caagctcgcc accgcctgcc gtgaggaaag atgcatgagc gagccgttaa ttgtcggcat
    13621 ccggcatcat agcccggcct gcgcccggct ggtgaaattg ttaatcgaaa gccagcggcc
    13681 acgatacgta ttgattgaag gcccggctga ttttaatgac cgggtggacg aactgttttt
    13741 agcccaccag cttccggtag ctatttacag ttattgccag tatcaggacg gtgcggcccc
    13801 cgggcgtggt gcctggacgc catttgctga attttcgccg gagtggcagg cgctacaagc
    13861 cgcacgtcgt attcaggcac aaacttactt catcgatttg ccttgctggg cgcagagtga
    13921 agaagaagac gaatcacccg atacgcaaga tgacagccag accttactgc tgcgtgccac
    13981 ctgcatggat aacagcgata ccctgtggga tcacttattc gaagatgaaa gccagcaaac
    14041 tgcattaccc tctacgctgg cgcactattt tgctcaactg cggagcgatt cccccggaga
    14101 tgcgctcaat cgtcagcgcg aagcctttat ggctcgctgg attacatggg cgatgcagca
    14161 aaacaatggc gacgtgttag tcgtctgcgg cggctggcac gctcctgcac tggcaaaaat
    14221 gtggcgcgaa tgcccgcagg aaattaacaa gccagaattg ccctcgctgg cagatgccgt
    14281 tactggttgt tatctcacac cctacagtga aaagcgcctt gatgtgctgg caggatacct
    14341 ttccggaatg cctgccccgg tctggcaaaa ctggtgctgg cagtgtggct tacagcaggc
    14401 aggtgaacaa ctgctgaaaa cggttcttac ccgtttgcgc cagcacaaat tgcccgcttc
    14461 taccgctgat atggctgctg ctcatctgca tgctatggca ctggcacagt tgcgcggcca
    14521 tacactacct ctacgcactg actggctgga tgccatagct ggttcgctga ttaaagaagc
    14581 cctgaatgcg ccgttgccgt ggagctaccg cggagtcatt aatcccgata ccgatccgat
    14641 tctggtaacg ttgatagaca cattagcggg tgacggattc ggtaaacttg ccccttctac
    14701 gtcacaaccg cctctgccaa aagatgtcac ctgcgaactg gaacgtaccg gaatctctct
    14761 tccggcggag cttaccttaa atcgctttac ccccgatgga ctggcgcaaa gtcaggtgtt
    14821 acatcggttg gcaatactgg agatcccagg ggttgtgcgc cagcagggaa gtacactgtc
    14881 acttgcaggc aacggtgaag aacgctggaa attaacccgg ccacttagcc agcatgcggc
    14941 attgattgaa gccgcatgct ttggcgccac actccaggaa gccgcacgcc ataaattaga
    15001 agccgatatg ctggacgcgg gtggaatcgg ctgtatcacc acatgtctta gccaggcagc
    15061 gttagcgggt ctggcgtctt tcagtcagca attactggaa caactcacat tattaatcgc
    15121 ccaggaaaat caattcgccg aaatgggcca ggcgctggaa gtgctatatg ccttatggcg
    15181 gctggatgaa attagcggta tgcaaggcgc gcagatatta caaacgacat tatgcgcggc
    15241 catcgatcgc acgctgtggc tatgtgaatc caacggcaga ccggatgaaa aggagtttca
    15301 cactcaccta catagctggc aggcgctttg ccatatcctg cgcgatctac atagcggcgt
    15361 taatttaccc ggcgtttcgc tttctgcggc agtagcctta ctggagcgcc gcagtcaggc
    15421 gattcatgcc ccggcgctgg atcgcggcgc gactcttggc gcactgatgc gtctggaacc
    15481 tcccaacgcc agtgccgaaa cggcgctgac gatgctggcg cagttatccc cggcacaatc
    15541 cggcgaggcg ctgcacggtt tgctggcatt agcccgtcat caactggcct gtcagccggt
    15601 atttatcgcc ggtttcagca gtcatttaaa tcaactgagt gatgccgatt ttatcaatgc
    15661 cctgcccgat ttacgcgcgg cgatggcctg gctaccacct cgagaacgcg ggacgctggc
    15721 gcatcaggtg cttgagcatt atcaactggt gcaacttccc gtttcggcac tgcaaatgcc
    15781 gttgcattgt ccaccacaag acattgcaca tcatcaacaa ctcgaacagc aggcactggc
    15841 atcgctgcaa cactggggag ttttccatgt ctgaactgaa cgatcttctg accacccgtg
    15901 agctacaacg ctggcgatta attcttggcg aagcggcaga aacgacgctt tgtgggctgg
    15961 atgacaacgc ccggcagata gaccacgcgc tggagtggct gtatgggcgc gatcctgaac
    16021 ggctccagcg tggtgaacgt tacggtggat taggtcgttc aaatctcacc acccctgagt
    16081 ggatcaacag cattcacacg ctgtttccgc aacaggtgat tgagcggctg gaaagcgatg
    16141 ccgtgctgcg ctacggcatt gaagatgtgg tgacgaatct cgacgtgctg gaacgtatgc
    16201 agccttctga aagcctgcta cgcgccgttt tgcacaccaa acatctgatg aatcccgaag
    16261 tactggctgc cgcccgccgg atagtgcacc aggttgttga agagattatg gcgcgactgg
    16321 caaaggaagt tcgtcaggct ttttctggag tacgcgatcg ccgtcgccgc tcatttattt
    16381 cactggcgcg aaactttgat ttcaaaagta ctctgcgcgc caacctgcaa cactggcacc
    16441 cgcaacacgg caagttgtat atcgaatccc cccgctttaa cagccgcatt aaacgccata
    16501 gcgaacaatg gcaactggtc ctactggttg atcaaagcgg atcaatggtg gactcggtga
    16561 tccactccgc agtgatggcg gcctgtttat ggcagttacc cggcattcgt acccatctgg
    16621 tcgcttttga cacaagtgtc gtcgatctca ccgccgacgt tgccgatccg gtagagctat
    16681 taatgaaagt gcaattaggc ggcgggacca atatcgccag tgccgtggag tatggtcggc
    16741 aacttattga acaacctgca aaaagtgtca ttattctcgt gagcgatttt tatgaaggtg
    16801 ggtcatcatc attgctgacg catcaggtga aaaagtgtgt ccagagcggc atcaaagtgc
    16861 tggggctggc agcgctcgac agcaccgcaa cgccttgcta tgaccacgat acggcccagg
    16921 cgctggttaa tgtcggcgca caaatagccg caatgacacc gggcgagctg gcttcctggc
    16981 ttgcggagaa tcttcagtca tgaattcact acgtccggaa ttattagaac tgacaccaca
    17041 ggcactgacg gcgttaagca atgccggttt tgttaagcgc agccttaagg aactggaaaa
    17101 tggcaacgtc ccggaaatca gtcatgagaa cggcgcttta atcgccacgt tcagtgacgg
    17161 tgtccgtacc cagctggcga acggccaggc actgaaagag gctcagtgca cctgcggggc
    17221 cagcggcatg tgccgtcatc gcgtgatgct ggtgttaagt tatcaacgac tttgtgccac
    17281 cgcccagcct acggaaaaaa aagaagaaga gtgggattcg gcaatctggc tgaaagaact
    17341 ggctaccttg cccgatgcca cccgcaaacg cgcgcaggcg ctggtcgcta aaggcatcac
    17401 cattgagttg ttctgtgcgc ctggcgaaat tccctctgcc cgattaccga tgagcgatgt
    17461 gcgtttttat tcccgcagca gcattcgctt cgcacgctgt gattgcattg aaggcacact
    17521 ttgcgaacat gtggtactgg ctgtgcaggc cttcgtcgag gccaaaactc agcaagcgga
    17581 atttactcat ttaatctggc agatgcgcag tgaacacgtc acatcatctg atgatccgtt
    17641 tgccagcgaa gaaggcaaaa cgtgtcgtca atatgttcag caattaagcc aggcattatg
    17701 gctgggcggg ataagtcaac cgccaatcca ttacgaggcc gctttcagtc gcgcgcaaca
    17761 agcggcggaa cgctgcaact ggcgatgggt gagtgaatcg ctacggcaat tgcgcgccag
    17821 tgttgatgcc ttccacgccc gtgccagcca ctaccatgcc ggagaatgct tacgtcagct
    17881 tgcggcatta aacagtcgat taaattgcgt acaagagatg gcgcgacgcg acagtatcgg
    17941 tgaagttcct cccatgccgt ggcgcacggt cgttggcgct ggtattgccg gagaagcaaa
    18001 acttgatcat ctgcggctgg tgtctttagg tatgcgttgc tggcaggata ttgagcagta
    18061 tggtttacgc atctggttta ccgatcccga cacaggaagt attttgcatc tttcgcgtag
    18121 ttggcagaga agcgaacagg aaaactcacc ggcagcgacg cgtcggctgt ttagttttca
    18181 ggctggcgca ctggcaggtg ggcaaattgt ttcacaagca gcaaaacgca gtgccgatgg
    18241 cgagctgctg ttcgcgaccc gtaaccgctt gagtagcgtt gttccactgt cgccagacgc
    18301 ctggcaaatg ttgagcgcgc cgttgcgcca gccgggcatt gtggctttgc gggaatattt
    18361 acgccagcgt ccccccgcct gcatacgacc actcaaccag gtcgataact tatttattct
    18421 gccggtcgcg gagtgtattt cgctcggttg ggatagcagc cgccagacac tggatgcgca
    18481 ggtaatcagc ggcgaagggg aagataatgt gctgacgtta tcccttccgg catctgcctg
    18541 ttctcctttt gccgttgaac gcatggcggc gcttttgcaa caaacagacg accccgtgag
    18601 cctggtttct ggctttgtca gttttgtaga ggggcaattg acactggaac cacgggtgat
    18661 gatgacaaaa acccgcgcct gggcgctgga cgcagaaact gcgcctgtgg caccgctacc
    18721 ttctgccagc gttctgcctg tgccgtctac cgctcatcag ttgctgatgc gctgccaggc
    18781 gttacttatt caactgctcc ataacggctg gcgctatcag gaacagagtg ctattggtca
    18841 ggcagagttg ctggcgaatg acctcaccgc ggtgggtttt tatcggctgg cacatgtgtt
    18901 gggacaattt cgtaatacag aaagcgaggc acgggtagaa gcaatgaata acggtgtttt
    18961 gctttgcgaa caattattcc ccatgcttca gcaacaagga tgaaatagtg ctttttatta
    19021 agagttctac tcctgttccg gactgctcac gccacagtat taggcatatc ctatatagcc
    19081 cctggtgaga gtcaccagtt ccttgattaa ataaaatgga gttttacatg aaggctttca
    19141 ataagctgtt ttcccttgtt gttgcatctg ttctggtttt ctctcttgct ggctgcggtg
    19201 acaaagaaga atcaaagaaa ttcagcgcca atctgaacgg cactgaaatt gccattacct
    19261 atgtctacaa aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct
    19321 ccattggtgc aaccaccaaa gaagatgccg ccaggacact tgagccgtta agcgctaaat
    19381 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
    19441 acgtgactat tgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
    19501 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
    19561 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
    19621 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
    19681 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgccgt
    19741 aatttccggc atcgaaacgc ggaactgctg ttcggtaagg attgccagaa tgtcatcggt
    19801 tttaagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc
    19861 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
    19921 gaatgaagac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
    19981 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
    20041 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
    20101 gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
    20161 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
    20221 tcaactcggc ctggccgtta tcttccagac ggatctcctg taaatgcgcg aggttaacca
    20281 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcagtt
    20341 cggtaaagcc ctctttccct tcgtgactgg taacgtagac accgctcatc cgactgctga
    20401 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
    20461 atttcagcgc ctgttgattt tccggtaaca acgaaacatc ctgcttgctg cgctcctgac
    20521 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
    20581 aggcatgttc ttcaaaggct ttaatggcgt attcgtcaaa cgcggtgaga aaaacaatat
    20641 acgggcgatg ttccgggtcg agcatcccca ccatttccag accactgatg cgcggcatct
    20701 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttctacgg
    20761 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt
    20821 tctcccgtgc aagcggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
    20881 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttatcccata
    20941 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
    21001 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
    21061 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
    21121 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
    21181 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
    21241 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
    21301 gtttttgcgg aaaaaagtag aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
    21361 gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatggggat tcacctgggc
    21421 gtgaagcagt ttgatctctg actgggtgag catcgctttt tgccgctcat attgcccggc
    21481 gaggatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt tgattgaact
    21541 gaataaacgg tttttggcct catacaattt gatggtaccc atcactcgct gattttcacc
    21601 acgcaacgga atgaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
    21661 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtata
    21721 agtcgaagaa atcggtttgc cgggtaaatg gtggtcgcca ccaattccgg taaaggccag
    21781 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
    21841 tgccactttc atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc
    21901 cgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
    21961 atcgagcaat atacgcataa acaacgccgc gccgacggta ttggtgacca tcattggcgc
    22021 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
    22081 caacatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
    22141 tttatcagtg cgcccgcgac ggataaggat gctgtgtacc aggccgccga gcaatccttc
    22201 aacgatggtc gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
    22261 cccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
    22321 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
    22381 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
    22441 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa
    22501 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
    22561 cacttccctt taaaacgcgt aacatacatt gcttgcgttt aactttcttt gaactcttgc
    22621 agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc
    22681 ctatagatgc tcaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
    22741 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
    22801 tgctttgtga tattaaccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga
    22861 aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt gaccggatcc
    22921 gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagtaaaa atgctgctca
    22981 gtaatgaaaa tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgactt
    23041 acctgcaaag cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt
    23101 accccgccca gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc
    23161 aacaaccgac tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatct
    23221 ctatttgcct ggcttcggcg cgcaaaaaac agggtaaaga tgcactggtg gttggctgga
    23281 atattcagga taccactcgt ctgtggctgg agggctggat tgccagtcaa caaggatggc
    23341 gcattgatgt cctcgcccac tcgctcaatc aactacgccc tgaactgttc gaaggccgta
    23401 cattgctggt atggtgcggt gataatcgaa cctccgccca acagcagcaa ctcaccagtt
    23461 ggcaggaaca aggccatgat attttcccac tcggcattta atgattcgtt aacaaatacg
    23521 ctttactgta caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg
    23581 ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg
    23641 ttataccttt attttgcgtg ctggctgaac gcctgcacca gcctttcaaa caggcggtct
    23701 gcgatgatcg ccgccagtgc caccagtaac gctccctgga tcacatacgc ggtattaaat
    23761 ccgctaagcc cgatgatgat cggcgtaccc agcgtgttgg cccctaccgt tgaggcgatc
    23821 gtcgccgtgc caatgttgat aatcaccgaa gttcgtacac ccgccagaat caccggagct
    23881 gccagcggta gctcgacctt acgcactcgc tgaccacgac tcattcccat acctttcgca
    23941 acttctgtca cgctggcatc aatcgctccc agcccggcaa gtgtcgcctg caggacgggc
    24001 agcacgccgt aaaggatcaa ggcgataatc gctggtttca gaccaaagcc gatcaccgga
    24061 acggcaatcg ccagcaccgc gacgggcgga aaagtctgcc caacggcggc aatagtttcc
    24121 accagtgggc gaaattccgc accccacggg cgagtgacag caattccggc accagtacca
    24181 atgatcaccg caaacaaact cgaaattccc accagccaga aatgagccag tgccagagct
    24241 gcaaagcttt cctgctgata aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc
    24301 ggctggctgt aaggcagcca gaaaatcagc gccacaaaca gagcaatcag ccagaacagc
    24361 ggatcgcgca acatcttcat acgattacgc ctcctccagc agatcctgaa aatgcagcgt
    24421 gccgcaaggc tcgccctgcg tgttcaccac cggcagcacc tcgcatcccc gcgcgacaaa
    24481 cagagaaagc gcatcgcgta gcgtcatcct ttcttccagt gcctcacctt cagcccgttc
    24541 ttcacggcgc acgtaatctg ccacgctacg taacgaaagc aggcgtacac ccagctcact
    24601 acgaccaaaa aactggcgga caaaatcatt cgccggacga gtcagcatcg tcagcggatt
    24661 accctgctgc accacttcac cgtgatccat caatacaaga tgttctgcca gccgtagcgc
    24721 ctcatcaata tcatgagtga ccagcacaat agtgcgcccc tgcaaacggt gaatgcgcgt
    24781 catctcttgt tgcaacgcgc cacgcgttac cgggtccagc gcgccgaatg gttcatccat
    24841 cagcaagact tgcggatcgg cagccagcgc gcgcgccact cccacacgtt gctgctgacc
    24901 accggaaagc tgatgcggat aacgctcacg caaatttggc tccagcccca gtagcgccat
    24961 taattcgtcg atacgatcgt cgatccgcgc ccgtgaccat ttttgtaatt gcggcacggt
    25021 ggcaatattt tgcgccacgc tccagtgggg aaacaggcca atagattgaa tggcatagcc
    25081 catccggcgg cgcaactcca gcactggcag cgagcgaatt tcttctccgg caaagcggat
    25141 caccccgctg tcatgctcca ccaggcggtt aatcattttc agggtagtgg atttaccgga
    25201 acccgacgtg ccaatcagta ccgaaaaact cccttcctga aaattgagat tgagatcgtt
    25261 aacggctttt tgtgcgccga acagtttgct gacatggcta aattcaatca ttacgtttca
    25321 ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt aagcaccgcc agaacaatta
    25381 ccgggatcac ccccagcaac actaaatcaa tggcactgct taacagcccc tgaaaaacca
    25441 gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac cgccatgcca acggtttgca
    25501 ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc cagcggtaac tgaacatgca
    25561 ggaaacgctg cgtcccgctc atccccatcg ctctggcgct ctccagcaca tcgcgcggga
    25621 tctggttcaa gccgactact acgccgcgca ccagcggtaa cagggcatag agcaccagtg
    25681 caatcagagc gggtgtcatt ccggttcctg ctatgccgag cgttcccagc cacggaaagg
    25741 ccgtaaccag cgcggcaagc ggcgcaatca acaggccaaa gagcgctacc gaaggcacgg
    25801 tctgaatgac attcagcaga gaaaaaatcg ccccttgccg cgcggtggaa aagtagcacc
    25861 agatgcccaa cggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca
    25921 gatgttgtgc cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattaacg
    25981 agagatcgtt aagcgtgccg gagtacagca accacagcgg aacaatggca atctgcatat
    26041 gcaacaacca gcgccacagc ggatgcgtgg agattcggcg gatggcatcg ctacaggcca
    26101 gcagtgccag cgccgcagcc agccagaaac cactgccgag gctggtacgc gccagcgcac
    26161 tgccattttg cgccagttgt gtcgccgcct ttccagctcc ccacaccagc aatacgaaga
    26221 cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt agcgggaata aaacaggctg
    26281 tcagccaggc gcaaccaacg cccaccagca tccagagagt ttgcggccac agctgccaga
    26341 gatgacgccc ctcacccgaa actaaacgat taggtgcgta actgataaac ggcagcgccg
    26401 ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg attaatacgg aaataagtca
    26461 cgggaaatta cttcgtccac cctttttgtt tcaggtagtc ggctgccact tttttggcat
    26521 ccagtccttc cactgcaatg ctggcattca gctgctgcaa tgttttttca tcgaggctgg
    26581 cgaagactgg ctgtagccac tgtgctattt gcggatactc tttcagcacc gactcacgca
    26641 ccactggcgc aggcgcgtag ataggttgca cgccttgcgg atcgcttaag gtttgcagcc
    26701 ccagcgccgc aaccgggccg tcggtgccgt aagccattgc ggcattaaca ccagaagttt
    26761 gctgggcagc ggctttgatc gtcaccgccg tgtcaccacc ggccagtgac agcaactgat
    26821 cctgaccgag cttaaagcca taggcttttt caaacgcggg taaggcatcg gcgcgttcaa
    26881 taaactctgc cgaggctgcc agtttgaagt taccaccctc tttcagataa cgactcagct
    26941 cggcaagcga agtgagtttg tttttctctg ccacatcctg acgcacggcg atggtccagg
    27001 tgttatttgc aggcgcgggc gtcagccaga ttaacttgtt ttgctctgca tcgagttttt
    27061 tgactttctc atagccttgc tgcgcgtttt tccatgccgt atcgttttca tctttaaaga
    27121 aaaacgcgcc attgccagta tattccggat agatatccag ctcgccagaa gtgatcgccc
    27181 cgcgcactac gggagtcgtt cccagctgca ctttatttac cgtaggaacg ccgtgacttt
    27241 ccagcacctg caaaatgata ttgccgagca gcgcgccttc cgtatcgatt tttgaaccaa
    27301 ccttaacggg ggaagccgcc tgtagcggca ggctcacggc tgccaacaaa accagtgaac
    27361 ctgcccagac ctttgagagt ggcatgatgc tttcctcatt cttttactgt tgttttcagc
    27421 gaattaagag aaaagcatag ttgataatgg cggagttagc ctgactgggt ggactcagtt
    27481 gcagaatcag ataaatactt aaaaggcata ttttgcccgg cgtttcttgc cggatgcggc
    27541 gtaaacgcct tatccggcat acaaacatcg aaaattacaa caactcaaac tcgcctttct
    27601 taacgcgtgc ggaatcagtg ccgatgaaga cattgaactt gccaggctcg gcatcatatt
    27661 tcatctgttg attccagaac ttcagcgcct caatatcgat tgggaagctg acggtctgag
    27721 tttcgcccgg tttcagggtg attttctcaa agcctttaag ctgcttcacc ggacgactca
    27781 tggaagccgt cacatcctgc aggtacatct gcactaccgt cgccccttcg cgcttaccgg
    27841 tgttcgtcac ctgcacgctg gccgtcactt tgccgtcacg cttcatggtc ggcgcagaaa
    27901 gtttcacatc agagacggtg aaagtggtat agctcagacc atagccgaac ggataaagcg
    27961 ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg gcattatacg
    28021 gacgcccggt attcagatgg ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg
    28081 acatcggcag cttgccggac gggttataat cgccaaacaa tacatcggca attgcattac
    28141 cgccttcagt ccccgcaaac caggtttcca gaatcgcatc agcctgctga tcttctttca
    28201 ccagcgccag cggacgcccg ttcatcagca ccagcaccag cggtttaccg gtggctttca
    28261 gcgccgcaat caagtcacgt tggctttgcg gaatagtgat atcggtacga ctggaagctt
    28321 cgtgcgccat cccctgagct tcaccgacca cagccaccac cacatcagat tgtttcgcgg
    28381 tttgcactgc ttcatcaatc atctcttgcg gcgaacgcgg atcaactttg accgcctctt
    28441 catactgatt caggaaatcg ataatgcctt tgtcactggt aacgttcgcc cctttggcat
    28501 acagcacttt accgttttca ccgacggcgt ttttaatccc tgttagcaca gtaacagatt
    28561 gatcggcgac acctgccgcc gaccagcttc ccatcacgtc acgcttgctg tctgccagcg
    28621 ggccaaccac cgcaatggtt gcagattttt tcagcggcag cgtttcgaga cgatttttca
    28681 gcaacaccag gctttcgcgc gccacttcac gcgcttcttt gcggtgcagg cggctttcgg
    28741 cattggtatc caccgggtca gactctttcg gccccaaatg gctgtatgga tcgttaaaca
    28801 atcccatatc atatttaacg ttcagtacat ggcgggcagc atcgtccagc tcttccatcg
    28861 tcactttgcc ggatttgatc aacccaggca gatacttcga gtaatactcg tcgctcatgc
    28921 tcatgttgat tccggacttc agcgccacgc gtaccgcatc ctctggatct gccgccgtgc
    28981 catgtttaat cagctctttg attgcgccgt gatcggaaac ggtgatgcct ttaaagcccc
    29041 actggtcgcg cagaacatct ttcagcagcc aggagtcgga ggtggctggc gtgccgttca
    29101 gcgagttcag cgccaccatc accgcaccgc tgcctgcatc cagccccgct ttgtacggcg
    29161 gcatatagtc gttaaacagg cgctgcggac tcatatcgac ggtgttgtac tctttaccgc
    29221 cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag tagcgatctg
    29281 ccgggctttt accctgcatc gcttccacca tggttttgcc cattattgag gtgagatacg
    29341 tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca
    29401 tcggtgccca ggtcatattc aggccatcat ctgccgcttc ataagcagag acacgcccga
    29461 ctgttttcac cgcatcgagg ttaaaagacg aagccagacc gagactaatc gggaacaccg
    29521 tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg cggcttaatt
    29581 ccatcacctg atcctgcatg gcgcggatat cctgacgggt tacggtgttg aaaatcgccc
    29641 caacctgacc gtctttgatc atctcgcgga tcgcctcttt cgggttatcc gggccgacgc
    29701 tgattaagcg cagctgacca attttctcat caactgtcat tttcttaagc agttcggtga
    29761 cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca tccgccagtg
    29821 ctggctgcag ggccagactc accgcgattc ctactgaaca tagccatttc atatggattt
    29881 ttcttccttt gttgcccgac gtggcagcga aaatggtgca aaaaccgtag tttgccataa
    29941 gcatgataga gagagaaaaa gaatgctcag tttattgtct gaattttcaa aatattcact
    30001 cgctgaattg tcatacaagg cgctattcta gtttgtgata ttttttcgcc accacaagga
    30061 gtggaaaatg tcttccatga caacaactga taataaagcc tttttgaatg aacttgcccg
    30121 tctggtcggt cattcacacc tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg
    30181 cttccgttct ggtcagggcg acgcgcttgc tgtcgttttc cctggctcac tactggaatt
    30241 gtggcgggtg ctgaaagcct gcgtcaccgc tgacaaaatt attctgatgc aggctgccaa
    30301 tacaggcctg accgaaggat cgacgccaaa cggtaacgat tatgatcgcg atatcgtgat
    30361 catcagcacc ctgcgtctcg acaagctgca cgtactcggt aagggcgaac aggtgctggc
    30421 ctatccgggc accacgctct attcactgga aaaagccctc aaaccgctgg gacgcgaacc
    30481 gcactcagtg attggatcat cgtgtatagg cgcatcggtc atcggcggta tttgtaacaa
    30541 ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg
    30601 tataaatgaa gacggcaaac tgacgctggt gaaccatctg gggattgatc tgggcgaaac
    30661 gccggagcag atccttagca agctggatga cgatcgcatc aaagatgacg atgtgcgcca
    30721 cgatggtcgt cacgcccacg attatgacta tgtccaccgc gttcgtgata ttgaagccga
    30781 cacgcccgca cgttataacg ctgatccgga tcggttattt gaatcttctg gttgcgcagg
    30841 gaagctggcc gtctttgcgg tacgtcttga taccttcgaa gcggaaaaaa atcagcaggt
    30901 gttttatatc ggcaccaacc agccggaagt gctgaccgaa atccgccgtc atattctggc
    30961 taatttcgaa aatctgccgg ttgccgggga atatatgcac cgggatatct acgatattgc
    31021 ggaaaaatac ggcaaagaca ccttcctgat gattgataag ttaggcaccg acaagatgcc
    31081 gttcttcttt aatctcaagg gacgcaccga tgcgatgctg gagaaagtga aattcttccg
    31141 tccgcatttt accgaccgtg caatgcaaaa attcggtcac ctgttcccca gccatttacc
    31201 gccgcgcatg aaaaactggc gcgataaata cgagcatcat ctgctgttaa aaatggcggg
    31261 cgatggcgtc ggtgaagcca aatcgtggct agtggattat ttcaaacagg ccgagggcga
    31321 tttctttgtc tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc
    31381 gggcgcagca attcgttatc aggctgtgca ttccgatgaa gtcgaagaca ttctggcgct
    31441 ggatatcgct ctgcggcgta acgacaccga atggtatgag catttaccgc cggagatcga
    31501 cagccagctg gtgcacaagc tctattatgg ccattttatg tgctatgtct tccatcagga
    31561 ttacatcgtg aaaaaaggcg tggatgtgca tgtgttgaaa gaacagatgc tggaactgct
    31621 acagcagcgc ggcgcgcaat accctgccga gcataacgtc ggtcatttgt ataaagcacc
    31681 ggaaacgttg cagaagtttt atcgcgagaa cgatccgacc aacagtatga atccggggat
    31741 tggtaaaacc agtaagcgga aaaactggca ggaagtgaaa taaaaattac ggatggcaga
    31801 gtatcgccat ccgtacttca tttaatcgtt ctgtgccgtc tgccccgccg ccgccatttg
    31861 ggcggctttt tgttttttat agctcaacgc tgctgccgga acaggcatca ctttaccggt
    31921 ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg gtatatttgc caaacgcgtc
    31981 catcactacc agcgccaccg gtttattatt gataaccgtg cgcatcacca gacaatggcc
    32041 cgccgcattg gtaaaaccgg ttttggttaa ctgaatattc cagttatcac gatacaccag
    32101 atggttagta ttgcggaacg gcagcgtata cgtcgggtta gagaaggttg ccatatcttc
    32161 ccgggtagta cttaactgcc cgatcaacgg atattgtttg ctggcaatga gtagtttggt
    32221 taagtcacgg gcggttgaaa cgttatgcac cgacaatccg gttggttcaa caaagcgcgt
    32281 gttgttcatt ccgagcgatt tcgctttcgc gttcatcgcc ttaataaagg ctttgtaacc
    32341 accgggataa tggtgcgcaa gacttgccgc cgcgcggttt tctgaagaca tcagcgccag
    32401 caacagcata tctttacggc tgatttcgct gttcagtctg acacgcgaat agaccccttt
    32461 catctccggc gtctggctga tatccacttt tagtttttca tccagcggca gtcgtgcatc
    32521 cagcacaacc atcgcggtca ttaatttgct gatagacgca atcggacgca ccagatccgg
    32581 gtggttcgaa tagatcactt tgttggtatt cagatcaaca atcatcgcgc taccggaggc
    32641 aatttccggt tgtgacgcgg tggtagcggc taccgttttc gcaacggcct gcggtgcaaa
    32701 aggcacagcc agcatcaggg ccaggctaaa taaagaaact cgaaatttcg gcatgatgag
    32761 cattcagata gtggttcacg cgcacgggtt gcgcaccgcc ggagtaagga tttactgagg
    32821 ctagcgacgc catcataacg agcaaaaagt gcgatcgtca aagaagaatc gtgaggaaat
    32881 gctgcattgc tgacatttac gccagcaatg cagcgcaaaa gaactttcta gaacaaacga
    32941 taaccgtagc cccatagtat aacggtaagg gcgagcagca cttccagcac cagcacgcct
    33001 atcgccagtg tcgaactgga gaagctaagt ccttcctctt tgttgatatt caggaagctc
    33061 ggaataccga gatacagcag gtagccggtg taaaacagcg ccaccgtgcc gaccagcgca
    33121 cacaaccaga ccagcgggta aagcgccacc agaccgctta aaaacagcgg agttgcaacg
    33181 tagcccgcga agaccatgca gtgcgcaagt gacggacgct gcggataatt acgcgccatc
    33241 caccagatga cccgccccat caccgcaacc ccagccagca taacgccgta aaacaagaca
    33301 gccagcgcca gtccggtaaa ccaggataac ttcaggatag tgccatcgcc aaaattccag
    33361 ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa tcgccgccat cagcaaaacg
    33421 tggtgggtgt agtgatgaga aatcgtttcg ttttcgcgat taatcacctg catttcacga
    33481 tcgggatggg aaaacagtcc ccagacatgg ctcataccgc ccccttgttg tgagttcatg
    33541 aacctgacag ttcaagtata agtcagcttg tgattatttt ttgttcgctc atgtaatttg
    33601 caccgtctga tgtacgtcct ttttcagcgt gaatgattca cagggtgtat gcttacaggc
    33661 aagcaaaggg agacagactg tcctatggat ctcaatacac tcatctcaca atatggttat
    33721 gccgcgctgg tgatcggtag cctggcggaa ggtgaaaccg tgactttgct gggaggcgtt
    33781 gcggcacatc aggggctatt aaagttcccg ctggtggtac tttctgtggc gcttggcggc
    33841 atgattggcg accaggtgct ttatctgtgc gggcggcgct ttggcggcaa gctgttacgc
    33901 cgtttctcga aacatcagga taaaattgag cgggcgcaga aacttatcca acgccatccg
    33961 tatctgtttg tcattggtac gcgttttatg tatggctttc gggtgattgg cccgacgctg
    34021 attggtgcca gccagctgcc gccgaaaatc tttctgccgc tgaatattct cggcgcattt
    34081 gcctgggcgc tgatttttac cactatcggt tacgctggtg ggcaggtgat tgcgccgtgg
    34141 ttgcacaatc tcgaccagca tttgaagcac tgggtctggt tgattctggt cgtggttctg
    34201 gtggtaggcg tgcgctggtg gctgaaacgg cgcgggaaga aaaagccgga tcatcaggcg
    34261 taaaactatt gccctggata aggtgttcgc gccgcatccg acatccggtg caccatgcct
    34321 gatgcgacgc tggcgtgtct tttctggcct aacccaatga aataaccacg cacctcactg
    34381 cgctattctg ggttgaactg cggattcgcc aacataaagc cgccatccac tatcaacgac
    34441 tgcccggtgg tgtaatttgc gccctccgaa caaagccata ccaccaggct ggcaatctca
    34501 tgcgttgtgc caaaacgtcg caagggaatc gaaggctccg cgtcaggctt cacgtcgccg
    34561 ccatccatgc cgttcattgg cgtggcgatc gccccaggtg cgactgcgtt caccaaaatt
    34621 ttatgcctga ccaactccag cgccattgct ttggttaacc cgccgagcgc atgtttagcg
    34681 gctgtgtagg cgctggcatc cggcagcggc gtatgttcat gtaccgatgt aatgttgatg
    34741 atgcgaccgc cctgcccttg cttcaccatc tggcgggcag caatttgcga gcataaaaat
    34801 gcaccatcga catcaacggt aaaaatcttg cgccactcat cgaaagccat atcaagaaac
    34861 ggcgctttgg tcattgcgcc cgcattattc accagcacat caatacgccc cagccgttga
    34921 ataagtttct ccagcgcctg cgcaccttct ggtagattgc cgagatccag ttgcacgatc
    34981 tccgcacgta cgccgtggct aaccacctta cgcgcggtat cttttgcccc ttcttcatct
    35041 gagtgccagg taatgccaat atcaaacccc tgctgcgcca gtaataacgc gcactctttg
    35101 ccgatccccg aatcggaggc ggtaattatc gcgacctgtg ccatcgagtt ctccacttaa
    35161 cgctgaataa atgttaagta tagaaggcgc atatcatcag cgtttgtacc ccccgcccag
    35221 cgcaccggtg agttgaatgg aggcatccag ccactgccct tgcaataaca ggccattggc
    35281 ccgctcacgc agcgccggga ttctggcttc gctgacccgg gaaccagcaa tgaggcccgc
    35341 gttaaagcgg gcctgcgcaa gacctactac acgcaaggca tcgcgctcaa tttgcgcctg
    35401 atgctggttt ttctccgcca gtgtctgaac ctgactggct gcccgcgcca cgtcattcac
    35461 cgcttcaacc accgctttgt tgtagctggc gatagacaag ttgctttcgg ctttagcgat
    35521 atcgaggttg gcgttaagac gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac
    35581 gcccatttgc tgcgcggaat gacggaacag atcgcttaag tgcaacgcat cctgttgcag
    35641 aaaggccatc aggttgatgt caggataaaa tgccgctttt gccgcatcaa tagtgcttaa
    35701 cgatgactca acgtaccagt gcgccgcctg caaatctgcc cgccgcgcca gtaaggagta
    35761 ccccagttca tcaggaagct ggcttgccac tttcggcaac gcgaccggtt taagcttcag
    35821 tgactttgtc tggtgatttg tgagtgcatt taaccgtgcc tcaataattt tcattttacc
    35881 cgcgacatcg ttgagctgct gccgggtttt gctggcatta atatcggttt ccacaccttc
    35941 aactgaagaa gtaatcccgt tctgatatag ctggcgatcg gtcgcgataa tggtgttctg
    36001 ctctttttcg atttgctgca agaccgtgtt taacgccgcc tgggtttgcc actcccagta
    36061 caggcgggct acgctgccag ccagcaattg gcgggtttgc tcgcgttccg ccgcccgtgc
    36121 tttaaccgta cccagtcgag cagtaacctc cgcccgattc tttccccaga tatcgagatg
    36181 ccagcccgcc gttaagccaa aagtaccgtt ggtgtaccac ggaccggtcg tacctgcggc
    36241 cggatcgttc aaagcaaacg gccccattaa cccttctgcc gacatttttt gccgctccat
    36301 atccgcagaa aagtcgagct gcggaccatc ctgagtggca actgccttcg cctgggcttc
    36361 agccagctga atgcgctgtt cagccacctg catatacggt gcgttctgta gtgcattgtt
    36421 aattaatgaa gtgagttgat catcgtgata ctccagccac cattggctgt ctggccaacc
    36481 atttttcagt gccgtgggta atgcggtgtc aacttgcaca gcgagcgtgt gctggcttaa
    36541 cgcctggcgg gtttcatgca taggcgcaca cccggccagc atcagtaaca gcggaaaaca
    36601 ggcgatggcg ggataaaagg aatcacgatt catgggggaa taatcaggta agaaaaggtg
    36661 cgcggagatt accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat acatcagtgg
    36721 caataaaacg acatatccag aaaaatatac actaagcgaa tgatatctcc cgatttatct
    36781 taatcgttta tgaatagcgg caaagggctt cgttttttcc tatacttatt cagcactcac
    36841 aaataaagga acgccaatga aaattatact ctgggctgta ttgattattt tcctgattgg
    36901 gctactggtg gtgactggcg tatttaagat gatattttaa aaatcagata tggcatataa
    36961 gttgtcaggt caggaactag atattttgtg tacttgattt gcaaacagct ccggtgttgg
    37021 tggcgtcaaa atcgctgaag cgttccccga aggtcggggc agcccacatg gatgtgggct
    37081 gagggcgcgt cttacaagga cgttacctcg cgcccgaccc ggtagccgaa ggggataagc
    37141 tgaaggtacc gcgcagcggc gatttt
//
LOCUS       DAZFRE010000044        36343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFRE010000044 DAZFRE010000000
VERSION     DAZFRE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.044"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            131..775
                     /gene="adk"
                     /locus_tag="ACHKUI_002515"
     CDS             131..775
                     /gene="adk"
                     /locus_tag="ACHKUI_002515"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6598698.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            907..1869
                     /gene="hemH"
                     /locus_tag="ACHKUI_002516"
     CDS             907..1869
                     /gene="hemH"
                     /locus_tag="ACHKUI_002516"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6598699.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1844..2812)
                     /gene="aes"
                     /locus_tag="ACHKUI_002517"
     CDS             complement(1844..2812)
                     /gene="aes"
                     /locus_tag="ACHKUI_002517"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6598700.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDRPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            2964..4268
                     /gene="gsk"
                     /locus_tag="ACHKUI_002518"
     CDS             2964..4268
                     /gene="gsk"
                     /locus_tag="ACHKUI_002518"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6598701.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(4398..6074)
                     /gene="ybaL"
                     /locus_tag="ACHKUI_002519"
     CDS             complement(4398..6074)
                     /gene="ybaL"
                     /locus_tag="ACHKUI_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6598702.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(6312..7532)
                     /gene="fsr"
                     /locus_tag="ACHKUI_002520"
     CDS             complement(6312..7532)
                     /gene="fsr"
                     /locus_tag="ACHKUI_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO6598703.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            7750..9402
                     /gene="ushA"
                     /locus_tag="ACHKUI_002521"
     CDS             7750..9402
                     /gene="ushA"
                     /locus_tag="ACHKUI_002521"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6598704.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(9439..9918)
                     /gene="ybaK"
                     /locus_tag="ACHKUI_002522"
     CDS             complement(9439..9918)
                     /gene="ybaK"
                     /locus_tag="ACHKUI_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6598705.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVTVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(10122..10916)
                     /gene="ybaP"
                     /locus_tag="ACHKUI_002523"
     CDS             complement(10122..10916)
                     /gene="ybaP"
                     /locus_tag="ACHKUI_002523"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6598706.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AEKQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPSGRYVVAVGALHLYGEGNLPQMLR"
     gene            11049..11351
                     /locus_tag="ACHKUI_002524"
     CDS             11049..11351
                     /locus_tag="ACHKUI_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6598707.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            11387..11728
                     /gene="ybaQ"
                     /locus_tag="ACHKUI_002525"
     CDS             11387..11728
                     /gene="ybaQ"
                     /locus_tag="ACHKUI_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6598708.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(11786..14290)
                     /gene="copA"
                     /locus_tag="ACHKUI_002526"
     CDS             complement(11786..14290)
                     /gene="copA"
                     /locus_tag="ACHKUI_002526"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6598709.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            14552..15484
                     /gene="glsA"
                     /locus_tag="ACHKUI_002527"
     CDS             14552..15484
                     /gene="glsA"
                     /locus_tag="ACHKUI_002527"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6598710.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKD"
     gene            15487..16779
                     /gene="ybaT"
                     /locus_tag="ACHKUI_002528"
     CDS             15487..16779
                     /gene="ybaT"
                     /locus_tag="ACHKUI_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6598711.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            16810..16907
                     /locus_tag="ACHKUI_002529"
                     /pseudo
     CDS             16810..16907
                     /locus_tag="ACHKUI_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16904..17311
                     /gene="cueR"
                     /locus_tag="ACHKUI_002530"
     CDS             16904..17311
                     /gene="cueR"
                     /locus_tag="ACHKUI_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6598712.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(17315..17518)
                     /locus_tag="ACHKUI_002531"
     CDS             complement(17315..17518)
                     /locus_tag="ACHKUI_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598713.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTEIN"
     gene            complement(17515..17937)
                     /locus_tag="ACHKUI_002532"
     CDS             complement(17515..17937)
                     /locus_tag="ACHKUI_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598714.1"
                     /translation="MMNSSIKSFSLLAVILLTGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(18023..19072)
                     /locus_tag="ACHKUI_002533"
     CDS             complement(18023..19072)
                     /locus_tag="ACHKUI_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6598715.1"
                     /translation="MKADDAQHAIMVAQTDIDTNKASIADTRSDVSAVQSDVTNIKGD
                     VAHAQSTADHANANANTALMNGVKLSSAVTENKNNIEQNRSDIADQQKQLDETQKTVA
                     ATGDVQTAARYQSMIDARQTAAANAQQEQADTQQQQITDQQKQIDATQKTVSALGDAQ
                     TSAHYQEMVNARLSAQNDANARTAAEQKQKIDTLATNQETQQHIDSVQYGEQIQRLAQ
                     DSTQTHEQIDSLTQDATQTHQQLSNTQKRVADNSQQINTLNNHFSSLKNEVEDNRKEA
                     NAGTASAIAIASQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISAD
                     TQSDFGAGVGVGYSF"
     gene            19805..20167
                     /locus_tag="ACHKUI_002534"
     CDS             19805..20167
                     /locus_tag="ACHKUI_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598716.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(20270..20728)
                     /gene="ybbJ"
                     /locus_tag="ACHKUI_002535"
     CDS             complement(20270..20728)
                     /gene="ybbJ"
                     /locus_tag="ACHKUI_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6598717.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(20725..21642)
                     /gene="qmcA"
                     /locus_tag="ACHKUI_002536"
     CDS             complement(20725..21642)
                     /gene="qmcA"
                     /locus_tag="ACHKUI_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6598718.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            21788..22465
                     /gene="fetA"
                     /locus_tag="ACHKUI_002537"
     CDS             21788..22465
                     /gene="fetA"
                     /locus_tag="ACHKUI_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6598719.1"
                     /translation="MQENSPLLQLQNVGYLASDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVHEQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            22452..23231
                     /gene="fetB"
                     /locus_tag="ACHKUI_002538"
     CDS             22452..23231
                     /gene="fetB"
                     /locus_tag="ACHKUI_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6598720.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(23294..24148)
                     /gene="cnoX"
                     /locus_tag="ACHKUI_002539"
     CDS             complement(23294..24148)
                     /gene="cnoX"
                     /locus_tag="ACHKUI_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6598721.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(24209..25018)
                     /gene="ybbO"
                     /locus_tag="ACHKUI_002540"
     CDS             complement(24209..25018)
                     /gene="ybbO"
                     /locus_tag="ACHKUI_002540"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6598722.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(25008..25631)
                     /gene="tesA"
                     /locus_tag="ACHKUI_002541"
     CDS             complement(25008..25631)
                     /gene="tesA"
                     /locus_tag="ACHKUI_002541"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6598723.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVINASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            25602..26288
                     /gene="ybbA"
                     /locus_tag="ACHKUI_002542"
     CDS             25602..26288
                     /gene="ybbA"
                     /locus_tag="ACHKUI_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6598724.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEV"
     gene            26285..28699
                     /gene="ybbP"
                     /locus_tag="ACHKUI_002543"
     CDS             26285..28699
                     /gene="ybbP"
                     /locus_tag="ACHKUI_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6598725.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(28940..30034)
                     /gene="mnmH"
                     /locus_tag="ACHKUI_002544"
     CDS             complement(28940..30034)
                     /gene="mnmH"
                     /locus_tag="ACHKUI_002544"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6598726.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     GYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(30103..31029)
                     /gene="allS"
                     /locus_tag="ACHKUI_002545"
     CDS             complement(30103..31029)
                     /gene="allS"
                     /locus_tag="ACHKUI_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO6598727.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            31259..31741
                     /gene="allA"
                     /locus_tag="ACHKUI_002546"
     CDS             31259..31741
                     /gene="allA"
                     /locus_tag="ACHKUI_002546"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6598728.1"
                     /translation="MKLQVLPLSQEAFCAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            31819..32634
                     /gene="allR"
                     /locus_tag="ACHKUI_002547"
     CDS             31819..32634
                     /gene="allR"
                     /locus_tag="ACHKUI_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6598729.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            32724..34505
                     /gene="gcl"
                     /locus_tag="ACHKUI_002548"
     CDS             32724..34505
                     /gene="gcl"
                     /locus_tag="ACHKUI_002548"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO6598730.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            34518..35294
                     /gene="hyi"
                     /locus_tag="ACHKUI_002549"
     CDS             34518..35294
                     /gene="hyi"
                     /locus_tag="ACHKUI_002549"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO6598731.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMEPYR"
     gene            35394..36272
                     /gene="glxR"
                     /locus_tag="ACHKUI_002550"
     CDS             35394..36272
                     /gene="glxR"
                     /locus_tag="ACHKUI_002550"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6598732.1"
                     /translation="MKLGFIGLGIMGTPMAINLARADHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLT"
BASE COUNT         8682 a         9234 c         9616 g         8811 t
ORIGIN      
        1 tgcactttcc gcaaattatc ttgccattaa ccgtttcagc ctcaggtgcc tttcttgagg
       61 caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac attttaaggg
      121 gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag ggactcaggc
      181 tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata tgctgcgtgc
      241 tgcggtcaaa tctggctccg agctgggtaa acaagcaaag gacattatgg atgctggcaa
      301 actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc aggaagactg
      361 ccgcaacggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag acgcgatgaa
      421 agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg aactgattgt
      481 tgaccgtatc gtaggccgcc gcgttcacgc gccgtctggt cgtgtttatc acgttaaatt
      541 caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagagctga ctacccgtaa
      601 agacgatcag gaagagaccg tacgtaaacg tctggttgaa taccatcaga tgactgcacc
      661 gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg cgaaagttga
      721 cggcaccaag ccggtggctg aagttcgcgc agctctggaa aaaattctcg gctaatcaag
      781 ccgccgcaga tctgctctcg ggcaggtctg ctcatcctcc tcaggcaata cctcttacag
      841 caattagttc ttcttcttca cttttccgct acaattatca acaagttgaa tcgataagag
      901 gcggtaatgc gtcaaactaa aaccggtatc ctgctggcaa acctgggtac gcctgatgcc
      961 cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag acgcgtggtt
     1021 gatacctcac ggttgttatg gtggccgttg ctgcgcggcg tgattttgcc gctgcgttcg
     1081 ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc gctgatggtt
     1141 tacagccgcc agcaacagca ggcgctggcg caacgtttac cggagacgcc cgtggcgctg
     1201 ggaatgagct acggctcgcc atcactggaa agcgccgtag atgaacttct ggcagagcat
     1261 gtagatcata ttgtggtgct gccgctttat ccgcaatact cctgttcaac ggtcggtgcg
     1321 gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg gatatcgttt
     1381 attcgtgatt acgccgataa ccacgattat attaatgcac tggcgaacag cgtacgtgct
     1441 tcttttgcca aacatggcga accggatctg ctgttgctct cttatcatgg cattccccag
     1501 cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacaac tcgcgaactc
     1561 gcttccgcac tggagatggc accggaaaaa gtgatgatga cctttcagtc gcgctttggt
     1621 cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg agaaaaaggc
     1681 gtgggtcata ttcaggtgat gtgcccgggc tttgctgcgg attgtctgga gacgctggaa
     1741 gagattgccg agcaaaaccg tgaagtcttc ctcggcgctg gcgggaaaaa atacgaatat
     1801 attccggcgc ttaatgccac gccggagcat atcgaaatga tggctaatct tgttgcagcc
     1861 tatcgctaaa gaatcgagca ccgtcgcgta gagcatcgtc ggcggttttc atcatccgtg
     1921 aataatgcaa aaaggcgtgc agcataccgg agtagagttt gaactcacaa ggttgctggt
     1981 gtgccgctaa cgtctggtaa agcagacggc tgtcatccag cagtggatcg aactccgccc
     2041 cggcaataaa acagggcgga acttcgcgag tgagatcatt attaaacagg cagtaatatg
     2101 gcgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc atacatctgc aaatcctgtt
     2161 gcgttaagcc atcccagaca ccacccaaca gacgacgagt cacggaatcc cgtaatccgt
     2221 aaagcccata ccacagcaaa acgcccgcaa ctttaccgca atcgatctgt ttatcgcgca
     2281 accacaacgc actggcgagc gccagcatgg cacctgcgga atcaccagca aaaccaatgc
     2341 gggacatatt gatttgataa tcctccgcct gctggtggaa ataacaacag gcagccacaa
     2401 tttcctctat cgcttgcgga aaacgcgctt caggtgaaag ggtgtaatca atgccaatca
     2461 ccgtacattg gctgtagctt gccagcaggc gcatgatgcg atcgtgggta tcgagattgc
     2521 cgagaataaa accgcctcca tgcaaataaa atagcgtcgc cggcctatct ggctgcggat
     2581 aaaagatacg tgttttcacc tgcccatatt ttgttggaac cctgtaagct ctggttgcca
     2641 tttctggagc gcccacattc cagaatcggc gctcaagcgt gtaatactgt cgttgctcag
     2701 caatcgttcc cgttgcaggc cagggcggta aatccggctg aagagtattc acaacggtct
     2761 tcatttcagc agagataagg tccagaacgg gtaatttgtt ttccggcttc atgcaaaaac
     2821 tcctttcaaa ttacgtcatt gtaaggaacc actgccatga aaatgcgatc ccgcctgctg
     2881 atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca
     2941 gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa acgtaaatcc aaacattact
     3001 tccccgtaaa tgcacgcgat ccgctgcttc agcaattcca gccagaaaac gaaaccagcg
     3061 ccgcctgggt agtgggtatc gatcaaacgc tggtcgatat tgaagcgaaa gtggatgatg
     3121 aattcattga gcgttatgga ttaagcgccg ggcattcatt ggtgattgag gatgacgttg
     3181 ccgaagcgct ttatcaggaa ctaaaacaga aaaacctgat tacccatcag tttgcgggtg
     3241 gcaccattgg taacaccatg cacaactact cggtgctcgc ggacgaccgt tcggtgctgc
     3301 tgggcgtcat gtgcagcaat attgaaattg gcagctatgc ctatcgttac ctgtgtaaca
     3361 cctccagccg taccgatctt aactatctac aaggcgtgga tggcccaatt ggtcgttgct
     3421 ttacgctgat tggcgagtcc ggggaacgta cctttgctat cagccccggc cacatgaacc
     3481 agctgcgggc tgaaagtatt ccggaagatg tgattgccgg agcctcggca ctggttctca
     3541 cctcttatct ggtgcgttgc aagccgggtg aacccatgcc ggaagcaacc atgaaagcca
     3601 ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac gctgggcacc aagtttgtca
     3661 ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga ccacgtctct atccttgcga
     3721 tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga tccgttgttg gcatctgaca
     3781 aggcgctgga ctgggtagat ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg
     3841 caggctttac cgaagacgaa gcgaaacgta aaacccagca tccgctgctg ccgggtgcta
     3901 tagcggaatt taaccagtat gagtttagcc gcgccatgcg ccacaaggat tgccagaatc
     3961 cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg gccggaaaaa atcatgaaca
     4021 ctaatggagc aggggatggc gcactggcag cgttgctgca tgacattacc gccaacagct
     4081 accaccgtag caacgtacca aactccagca aacataaatt cacctggtta acttattcat
     4141 cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta tcaggtactg aaccagcatt
     4201 cacctcgttt aacgcgcggc ttgccggagc gtgaagacag cctggaagag tcttactggg
     4261 atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt tcacgccgca tctggcattt
     4321 tgctttcaat gcctgatgcg acgctggcgc gtcttatcag gcctacatat ccgcgcctat
     4381 tatccggcaa ccaccgttta ccctgtcacc acctcacccg ccggtggcgt ttccagcagt
     4441 tccagcatag tacgggcaat ttcacgctca cccatcacta cctgattcgc accacgttcg
     4501 gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt
     4561 ttcgcgcggg cagatgccac aatctcgccc gcttcatagc cgttgggaat cgtcaggatc
     4621 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc cgcattgccc
     4681 aatactgcgc ggaccccgcg ctcgcgcagc tcatcaacac gggttcgtga cgtctcaatc
     4741 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
     4801 taacccacca gtagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt
     4861 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtagt
     4921 gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt
     4981 ccggcctgcg gcagtaaatt caatgccatt cctagtcccg ccaggataaa cgcgaactca
     5041 ccaatctgcg ccaggctggc ggcgatggtt aacgccgtgc gctgggagtg accaaacaaa
     5101 cgcacaagga acagggccgc gaccgattta ccaaacagaa taattgccag cgtcgccagt
     5161 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
     5221 aacagcaccg cgaacgcatc gcgcaatggc agtgtatcgt gggcggcacg gtgactcagt
     5281 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
     5341 agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
     5401 tcgcgggaac cggttgctgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc
     5461 agcatcataa tggcgataaa tgcgatcacc ttgccgatgg tgatccccat atcgactgca
     5521 agagtggcaa agcccacatc gccctgttcc atcattcctg ctaccgcggg tagcaatacc
     5581 agtgtcagga ccatcaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
     5641 tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
     5701 gaaagacata agccgaacac gataccggtc attaacgacc agcccagcac ggcggagagc
     5761 gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat ggcgatggcc
     5821 tttaccgcca tcaaatcctt cagcgaaaag tgcagaccga cgccaaacat caacagaatg
     5881 acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
     5941 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
     6001 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
     6061 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa
     6121 accaaaatta caggtaatag tttatgacaa tttcattgat gatgtttatg aataattgtt
     6181 gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa acagaaggcg
     6241 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
     6301 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
     6361 agtggcagga aagcacagat tttatagact aactctatgc tggtgtggtc ggcgataagc
     6421 cccagaactg ccgcccccag acctcccatg ccgaaagcaa aaccgaaaaa cagtccagaa
     6481 accataccga tacgtcccgg aagcagctcc tgggcgtaga ccagaatggc agagaatgct
     6541 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
     6601 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgt
     6661 ccaattttat cccctacagg cccgccaatc accgtacctg ccgcaacggc aaacaggaag
     6721 gcaaacaaat gaagctgagc attctggata gataatccga atttttgcat cagataaaag
     6781 gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag gattaacaga
     6841 atgctgaccg ccagaacaac tttattgcgc ggcagtggat tgataatcgt cgctttgggt
     6901 tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgcgc caacaccacg
     6961 atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata aggcgcgata
     7021 atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt tgccgccgac ctgaaagata
     7081 gattgcgcca ggccatgacg cccgccggaa gccatacggg ccacgcgaga agattccgga
     7141 tgaaagaccg atgaaccggt accgaccagc gccgctgcca gcagaactgc gccaaaactg
     7201 cccgccagcg ccagcagcac cagaccactt aaggtaaaac acatgccaat cggcaacgac
     7261 cacggcatcg gatatttatc ggtccagtag ccgaccaccg gttgcagtag cgaagaggcg
     7321 agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa ttctgactga
     7381 agcagtggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag atgtgagagg
     7441 ctgatagcac ctaaaatacc aaacgatgtt cgggccttag tcgttgacgc agccgcgccc
     7501 gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca gggttgcgat
     7561 gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg cgccgcctga
     7621 aggaagtctc aacgcggaat acagaatttc taatctggat gcagatttat cttcaccgga
     7681 cgcagacttg tttatgatgt cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg
     7741 gagagaagta tgaaattatt gcagcggggc gtggcgttag cactgttaac cacatttaca
     7801 ctggcgagtg aaactgctct ggcgtacgag caggataaaa cctacaaaat tacagttctg
     7861 cacaccaatg atcatcatgg gcatttttgg cgcaatgaat atggtgaata tggtctggcg
     7921 gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg cggtagcgtg
     7981 ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtccgactt acaggatgcc
     8041 gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat cggtaatcat
     8101 gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggctaa gttcccgttg
     8161 ctttccgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc atgggcgctg
     8221 tttaagcgtc aggatctgaa aattgccgtt attggcctga cgacggatga cacagccaaa
     8281 attggtaatc cggaatattt cactgatatc gaatttcgta agcccgctga tgaagcgaag
     8341 ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc ggcgacccat
     8401 atggggcatt acgacaatgg tgagcacggc tctaacgcac cgggcgatgt ggagatggca
     8461 cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca agatccggtc
     8521 tgtatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc atgcaaaccg
     8581 gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata cgtgggacgg
     8641 gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct gattccggtg
     8701 aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct gtacacccct
     8761 gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa caaaggcaaa
     8821 gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga tcgtgacaaa
     8881 gtgcgttttg tacagaccaa tatggggcgg ttgattctgg cagcacaaat ggatcgcact
     8941 ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga agcaggtgat
     9001 atcagctata aaaacgtact gaaagtgcag ccattcggca atgtggtggt gtatgccgac
     9061 atgaccggta aagaggtgat tgattacttg accgccgtcg cgcagatgaa gccagattca
     9121 ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggtaa actgaacgac
     9181 ctgaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgcatggc gacattaaac
     9241 ttcaatgcca ccggcggtga tggctatccg cgccttgata acaaaccggg ctatgtgaat
     9301 accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc gccgctggat
     9361 gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatccgaaag tgccggatgt
     9421 tcgcatccgg cacaatgctt aatcgcgacg ggcgatatca gcaaatttgg catcgagtat
     9481 ctttgccagg tcgcttgccg ccagttcgat atccagaccg cgcttgccgc cggaaacata
     9541 aatagtggca aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt ttttctgccc
     9601 cagtgggcta atccctccaa ccagatatcc cgtcgaacgc tgcgcgacca tcggatcggc
     9661 catctcaact ttcttggcac ccagcgcttt tgctactttt ttaagatcca gttgacctgc
     9721 gaccggcgta acggccacgg caaggtgttt catatcaccg ttcactgtga ccagcagcgt
     9781 tttgtagacc tgatccggat tcaaacctaa ttttttgacg acttcatcgc caaaattggt
     9841 ttcagccgga tcgtgctcgt aggtgtggat ttgaaacgaa atcttgtttt tttcgagtaa
     9901 tttaactgcg ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa gcaagcatac
     9961 gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag agtattggca ggatggtgag
    10021 attgagcgac aatcgagtta caccgtcgct taaagtgacg gcataataat aaaaaaatga
    10081 aattcctctt tgacgggcca atagcgatat tggccatttt tttagcgcaa catttgtggc
    10141 aaattccctt cgccatacag gtgtagtgca ccgaccgcga ccacatagcg ccccgacggc
    10201 atggcgcgta atttatcccg ccaggcgaga tttcgctgat gcatcagcac atcgtacagc
    10261 gactgactga acgtattggg caacgttatt tcattatttt gcggcggtgc attcagccac
    10321 cagctcatca tttgttgcag caaccgtgcg ttggtgtgcc agtgggtcag cgtgtcgtcc
    10381 agcagcgcca gtcctttgtc agggagctgg agcagcatgg caatttgctt ttctgcgccc
    10441 tccagttcaa tcacgggttt atgttgttgc ttcgccgcct gcaatagctg gtaatcaata
    10501 ccatattctg cccgcagccc cagtttttgc gcctgcgtcg cctgaagaac catcgcgatt
    10561 tgccacagcg gttgggtaga aaagagcgac ggagaaatgc ccatctcctg gctaatgtgc
    10621 tgcaggtttt ggagttgctc ctcgctaatg cgctcttcca gtgcctcgca ggcaggcaaa
    10681 ttggcaaaag gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc atcggcgttt
    10741 ttgagctttt tgagcaaacg ggtgggcagg ggagccatat cgtggctacc catatgaata
    10801 ctgccgatca gatgaaaatg gcgattgccg ggaagagtga tatcgatagc aggccaggtg
    10861 taatgattac cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag atccatacga
    10921 cctccctttg tgaaatatca tgctagcgcg cggggaggga tggcgcaaat ggagttgatt
    10981 gttgcacttg cgcacagtgc ttgtgtgcat tacaatgatt gaataatgga caaggagatg
    11041 agagaggaat ggctcaaaag aaaaatataa ggagttttcg tgatgcctgg ttggctgatt
    11101 tttttgtcca ttcaacgcca catcgaaaga tccctgctga aatccatacc acgttatcac
    11161 gaaagttgga catcattaat gctgccactt ctcatcggga tttacgctcc cctccgggga
    11221 accgatacga agagttatcc gggaaattac aggaatactc ctccattaga gtgaataaac
    11281 agtaccgact gatttttaaa tgggttaatg gcaaggccga agaattgttt ttagacccac
    11341 acaactacta aaacatagct gtcgtattga cgagaaaggt aaaactatga aacaggcaac
    11401 aagaaaaccg acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt
    11461 gaaaatcaat gagttagcag agttgctgca tgttcatcgt aatagcgtca gtgcactgat
    11521 caataacaat cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac
    11581 cacagtcgat ttttggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa
    11641 catgcgtacc caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg
    11701 tgaagagcgt gcaaaaaagg tcgcgtagaa taatttccct ataacggatg cgccacccta
    11761 aagcagcgca tccgcaatga tgtacttatt ccttcggttt aaaccgcagc aagcggttgg
    11821 cgttactcac tacggtgatc gacgagagcg ccattgccgc tccggcaacc accgggttaa
    11881 gcagtgttcc agtgaacggc cacaaaatac cggcggcgac cggaatacca atgctgttgt
    11941 agataaacgc accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaattgcga
    12001 gcgcatccgc aacacccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
    12061 caacatcact gccgccaccc atcgcaatac cgacatccgc ctgagccagc gctggcgcat
    12121 cgttaatgcc gtcgcccacc attgctacct gacgtccttc actttgcaga cgtttgatcg
    12181 cttcggcttt accgtccggc agcacgccgg ctatcacctc atcaatccct gcttctttag
    12241 cgatcgcatt ggcggtggtc gggttatccc cggttagcat caccaggcga tatcccgctt
    12301 tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca
    12361 gggctactgc tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag
    12421 ctgaaatatc tgcttcaatt gctttggtat tgacctgttg gtcatttaac aacgcctgat
    12481 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
    12541 ggaaaccgtt gacctgcggt agctgcatat cacctgcttt atcgaggatc gcccgtgcca
    12601 gagggtggct ggaaccttgc tccagcgccg ccgccagacg caatgcttgc gcttcatcaa
    12661 tatcagcaaa tgtttttact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt
    12721 tatcgaacac tacggtgtcg agcgtactgg cgcgttgcag cgcgtcggcg tcccgtacca
    12781 gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagtc
    12841 ccagcgcaca cggacaggca ataatcagca ccgtggtggc aatcaccagg gtatagacaa
    12901 tctgcggtgc cggaccaaag aaataccaga ttgccgcact gatcagcgca atcaccacca
    12961 ctaccggcac aaatacggct gagattttat ccgccagctg accgatttct ggcttactgc
    13021 tctgggcctg gcgcaccata cgaataatcc gcgacagcgt agtatggctg ccaaccgcac
    13081 tggcgcgaaa cagcacgcta ccgtcctgca ccactgtccc ggcatggacg ctatcgcctt
    13141 cgcctttttg ctgcgggata ggttcgcccg tcagcatcgc ttcatccagc cacgcttcgc
    13201 cctgggtgat ctcaccatct accggtacgc gatcgccggt cgtcaggcgc agcaacatac
    13261 ctggctgcac ttccgccagc ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg
    13321 tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgcg
    13381 cttccagcat atggccgaga ttgatcagac cgataatcat cgcgctggct tcgtaataaa
    13441 gatgtcgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
    13501 acgcaacacc cgttcccagc gccaccagcg tatccatcgt cgccgcaccg ttcagcaggc
    13561 ttttccatgc gctgcggtaa aaatgcccgc cggcgaaaac cattaccgcc agggttatca
    13621 ggccgataac taaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccgatca
    13681 tcccccagac catcaccggg atacccaccg ccagcgcgac aattgcctgc cagcggaagc
    13741 gcttcatcgt agcgacggcg gtttcttgct ggcgctcgcg gcgtttagcg tcatcttcaa
    13801 tcgcttccgc gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
    13861 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
    13921 cgctttgcaa cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca
    13981 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
    14041 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
    14101 atgcgtcata acccgcttgt ttgatggttt cgattagctg ttctgcactg gcagtcccgg
    14161 taacgtgcgc ttcagtgata gacacatccg cctgttcaac atccgggcgc tgttcgagac
    14221 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag
    14281 tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct
    14341 tccagcaagg ggaaggtcaa gaaattaata aaccaggcgg gtaaaagtcc gtaaagatta
    14401 aaaaatcggc tcgatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa
    14461 gttgcgggtg ctaagttaaa tcaggatgca tgaaaatcgg caccggggtg aggaattacc
    14521 tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc
    14581 agtggatcag gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgattacat
    14641 tccctttctg gcgaatgtcc ctggtcaact ggcggcagtg gctatcgtga ccagcgatgg
    14701 caacgtctat agtgcgggtg acagtgatta ccgctttgcg ctggaatcca tctcgaaagt
    14761 ctgtacgtta gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaagttgg
    14821 cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa
    14881 accgctttcg ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga
    14941 aaatactgaa caacgctggc agcgaatttt acatatccaa caacaactgg ctggtgagca
    15001 ggtagcgctc tctgatgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc
    15061 cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga
    15121 cgtgtacacc cgtcagtgct ccacgctcat caatactatt gaactggcaa cgcttggcgc
    15181 gacgctggcg gcgggtggtg tgaatccgct gacgcataaa cgcgttcttc aggccgacaa
    15241 tgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg
    15301 ggcgtatcgc gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt
    15361 tcctggcgtg atgggaattg ccgcgttttc accaccgctg gacgaagaag gcaacagtgt
    15421 tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaagga
    15481 ctgatcatga tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc
    15541 gtttccatcg gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct
    15601 gcgttgctaa tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg
    15661 ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggtattatc
    15721 gacttctttc gtcgcggatt aggcaacggc gttttttcgc tggcgctctc gttattgtac
    15781 ctgttgacgc tggcggtgag tatcgccatg gtcgcccgtg cttttggcgc ttatgccgtg
    15841 cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc attggggatc
    15901 attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg
    15961 atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg
    16021 ctgcaaccgg cgcatatttc cgtttctgcg ccccccagtt ccggggcgtt cttctcctgt
    16081 atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa
    16141 gtgaaagatc cggaggtcat aatgccacgg gcgtttctgg tggcgattgg cgttaccacg
    16201 ttgctttata tctcgctggc gctggttttg cttagcgatg tatcggcatt agagttagaa
    16261 aaatatgccg ataccgccgt agcgcaggct gcttctccgc tcctcgggca tgtggggtat
    16321 gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccatcaacgc aaacctgttc
    16381 gccgtgttta acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat
    16441 aaacctctgt ggcggcagag tacctggggc aacatcatcg tcgtggtgtt gattatgctg
    16501 atgacggcgg cgctgaattt aggctcactc gccagcgttg ccagcgccac ctttttgatc
    16561 tgctatctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg
    16621 ccgattctta tcgttggtac gttggttatg ttgttggtga tcgttggctt tatctacagt
    16681 ctctggtccc agggcagccg tgcgctgata tggattattg gcgcactctt actcagcctt
    16741 attgtggcaa tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtact
    16801 tcctgtatta ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatt
    16861 tgttgaaacc ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca ttagcgatgt
    16921 agcaaaaatt accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt
    16981 gacgccgccg atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga
    17041 actgacctta ctgcgccagg cacgccaggt gggctttaac ctggaggaga gcggcgaact
    17101 ggtgaatctg tttaacgatc cgcagcgtca tagtgccgac gtcaaacgtc gtacgctgga
    17161 gaaggtggcg gagatcgaac ggcatatcga agaactgcaa tccatgcgca accagctact
    17221 ggcgctggcg aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct
    17281 ctccggctgc tgtcatcatc gggcggggtg aaaatcagtt aatctccgtt tgcaccgaaa
    17341 gaatatgctg caaactttct accataatgc gccccgactg aaataagcct tcctggattt
    17401 cgttgttaac attcacagcg tgaaccaggg aggctaataa caccacttgc aagaaggcaa
    17461 aaataaacgc accgataatc ataatatgcg tcagggggat tttcctttgc tccttcattt
    17521 taaataaccc acaaactgac cttccttcat taaggcgact tttcccgtat ggtgataaac
    17581 aatgacgtta taaagacctg actgataaac agtggcatct ttattgtctt catcaattgc
    17641 acataatgaa tcattgagat aagcaaagtt cttactgctc attctaaagg tcatgccaaa
    17701 tcctttataa atggctttct ttgccgcctg ggcatgttgt gcggcttctg catttttaaa
    17761 tgcctgcgcc tcggatgcac ttaaaatagc cgtctgacgc tgttgttctg cgagataaca
    17821 ggtgtcatgg ctgacgccct gcgcctggca atctgcgatg cgggaagtgg gtgaactaca
    17881 gccagtcagt aatataaccg ccagcaggga aaacgattta atggagctat tcatcattgt
    17941 taaccttaaa taatgtgcga attaatgaag aggtgaaacc cggcaagctt ccttgctctt
    18001 atttatattg aggattgaaa tattagaacg aatatcctac gccgacacct gcgccgaaat
    18061 cagattgcgt atccgcagaa ataccggctt taagtaccgt atgcgttccg gcattaaaag
    18121 atgttccgac agacaccgca gattcaccgt tgaaggttcc cgctcccgct gacaccatca
    18181 tcaagtcacc ggttttcacc tgtggttgtg aggcgatagc gatggctgat gcagttcccg
    18241 cattggcttc tttacggttg tcctcaactt cgtttttcag cgaactgaaa tggttattga
    18301 gcgtgttaat ttgttggctg ttatctgcga cccgtttttg cgtgttgctt aactgctgat
    18361 gcgtttgggt tgcgtcttgt gtcaggctgt caatttgttc atgcgtttgc gttgagtctt
    18421 gcgccagacg ctgaatttgt tccccgtact gcacactatc gatatgttgt tgcgtttcct
    18481 ggttggtcgc aagggtatca attttctgtt tttgttctgc agcagtacgc gcatttgcat
    18541 cattttgtgc gctcagtctg gcgttaacca tttcctgata gtgtgcgctg gtttgggcat
    18601 cgccaagtgc ggaaaccgtt ttttgtgtgg cgtcgatttg cttctgctga tcggtaattt
    18661 gttgctgctg ggtgtccgct tgttcctgtt gcgcattggc agcggctgtc tgtctggcgt
    18721 caatcatgct ctgatatcgg gcggcagtct gtacatcacc ggtggcagca acggtttttt
    18781 gggtttcgtc gagctgtttc tgttggtcag caatatcgct gcggttctgc tcgatattgt
    18841 ttttgttttc tgtcacagca gaggagagtt tgacgccgtt catcagagcg gtgttggcgt
    18901 tagcgttggc atggtcagcc gttgactggg catgtgcgac atcgcctttt atgttggtga
    18961 cgtctgactg cactgcggag acatcactgc gggtgtcggc aatggaggct ttattggtat
    19021 caatatccgt ctgcgccacc ataatggcgt gctgcgcgtc atcagctttc agcgatgccc
    19081 agttggctgt gtgctgtgcg ttctgaatgg cggtatcctg cccattgtcg cggtcgatct
    19141 gcgcttgatt ggttatcggg ttcagcgtac tggcgggctg tacgggagaa gaagttctat
    19201 tttcctgagg cgtcgttgtc tggtaatcat gcgcaatcag ataattttgt gcggctgcgc
    19261 tattttctgt cctttctaaa aatcgcccaa ttttctgatc aatttccatt tgcgacattc
    19321 ctggctggat tgccgctaat tctgtcgctg ccgcctcaac agtatctccc gcaaacgcag
    19381 atggacttac cgcggtagca acaacgacag ccagtaaaga caactttaca gtattcatgc
    19441 attttttctc gttcaaagta agtcacacat ttgtttctat aagcaacgta aagtgttgtt
    19501 atgagaattg atctgcgttg caatttaatc gaaatctacg ccgccatttg tgtgatgtaa
    19561 agcgcatttt tgtctggtgt aagcgtgatt accaccgata agaaacaaaa aatgtttctt
    19621 atataaataa aataggaaat cattttgtgc tttttcaatg taaaaaatag taaagacttg
    19681 ttttgatgag cgaaataaat gttgtttctg tgctaaacgt gaagcggttc ctgatgtgtg
    19741 ctagaatgcg tttctttgat aaatctcaat ttttaagagg tttacggcag gcaggaaaat
    19801 ttaaatgaat gaatttgaga agatttttaa tgaaatgaat ctggaccggg cattgctacc
    19861 gatccttttt cgttcgaacc ggtcgacagt gtggaagtat ttaagcggcg atagtacggc
    19921 tcctgcctct gccatgtcgc tgattatgct gttgcagttg atccagaaac gaaaccccga
    19981 tctgttagcc gaatggttaa cgctttctga tttcaccatt ccgccagaag tttacctgga
    20041 ccaacctgat tactggaaag ggtgggtgta tacccaacac aaggtcaata aaaacgtcct
    20101 ggaatatcta aaaaaacact atccggatga agaccaaaaa agtatgggta aaggccggga
    20161 agagtaattt ttcctctact cggctatatg ccggatgcgg cgcaagcgcc ttatctggcc
    20221 tacgattcgg gcatattttg taggcatgat aagacgcgcc agcgtcgcat caggcgatga
    20281 ccgcacggat aatcagcgtt attccttcta tcgcaatcac ctcaacatgc gtaccagcgc
    20341 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccacgcc
    20401 cgttgaccag cggagattcc agaacaaaac gtcggccaat caactgctgc ccgcgctggt
    20461 ttaaatgact gtcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    20521 gccaggcggc gagcagcgtc aggacggcaa acattacccc ttgccactcc caacccagcg
    20581 gcagcaacca gaccaccaga ccggtaatca ctgctgccac gccgctccac aacagataac
    20641 cattgccgcc caacatctcg gctgccagca gcaaaccgcc gagacttagc cagaaaatat
    20701 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    20761 cagctcagca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    20821 cactttgctg ttactggacg aaccgatctg ctgtaacgct tcggtgtatt tctgtgcgac
    20881 gaagtagttc accgcctgaa tatcaccgga ggcgatggct tcagacacca ttttggtggc
    20941 gcgggcttct gcttcagcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    21001 ttcgccttcc gctttcagga tttgtgactg tttttcacct tcggctttga ggatttccgc
    21061 ctgacggatc ccttccgctt caaggatgta agcgcgtttg gtacgttccg ctttcatctg
    21121 cgcgttcatt gaagagataa gctctgcggg cgggcgcaca tcacgaattt cgatacgggt
    21181 gactttaatc ccccacgggt tggtggcctc atcgacaata tgcagcagac gcgagttaat
    21241 gctgtcgcgc tgagagagca tttcatcaag ttccattgaa cccagcacgg tacggatgtt
    21301 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    21361 cgcgtcaatc acctgaataa agcacacagc gtcgatggta acgttggcgt tatctttcga
    21421 gataacttcc tgggaaggga tgtcgagcac ttgctccatc atattgatct tgcgaccaat
    21481 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    21541 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    21601 gaccagcgcg acaaaaatga gaatagggat aaagataagc atcggaaaaa cctcctgttg
    21661 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    21721 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    21781 aacagtgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcgag
    21841 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    21901 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    21961 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    22021 ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg atacggtata
    22081 cgataatctg atctttccct ggcagatccg taaccagcag cctgacccag ccatttttct
    22141 cgattttctc gaacgcttcg ctttgccgga caccattttg acgaaaaata tcgccgagct
    22201 atctgggggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta tgccgaaggt
    22261 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    22321 gatgatccat cgttatgtgc acgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    22381 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    22441 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attggcactg gcattaatgc
    22501 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    22561 tctggagcgt cgggcgcgcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    22621 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    22681 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    22741 ttgccattac ggtcggggcg ggaattactc tggcggtgct gattttatcg ggatcgattg
    22801 agtttatccc gatgcaggtg atccctatcg ccgggatgat agccggtaac gccatggtag
    22861 cggtggggtt gtgttacaac aatttagggc aacgggttat tagcgaacag caacagattc
    22921 aggagaaact gagtcttggc gcgacgccga aacaggcttc agcgatattg attcgcgaca
    22981 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    23041 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    23101 atcagattat ggtgaccttt atgctgctct caactgccag cctgtcgacc attattgcct
    23161 gctatttaac ctatcgtaaa ttttataatt cgcgccacca attggtggtg acgcaattga
    23221 agaagaaatg atgcaatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    23281 gatcaacaaa acatcaatac aacaacgcat acagctggcg gcgatacttc gacgccagtg
    23341 catcacccgt acccagcgca gcaaggatct cctggaacgt tttacgtgtc tgaccgtctg
    23401 cggcggtgag atctttacgc agatgcccaa acagtaactc cagcgcctct tcattgcgcc
    23461 caacctggtg cagttgcagt gccagttgcg ttgccagtgc ggcatcttct ggattctctg
    23521 ccacctgctg ttgcaactgt tgaatttccg gcgtatctgc cgcctgcttc agcagttcga
    23581 tttgcgccac cagtccctgg tagcgggtgt cctgatcctg caatggaatg gttttaagca
    23641 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    23701 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcaag gcgtcggtgt
    23761 aattgccttc ctgcatcagc tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    23821 gcactttatc cagcagggca cggatcgcct cttccggttg cggcccctgg aagccgtcta
    23881 ctggttgccc gttctggaac agatatacgg tcggaatcgc gcgcagacca aactgggcgg
    23941 caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    24001 ccgcgaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    24061 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    24121 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    24181 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    24241 ccggcagtag gcgcttaagc accattaccg cccaggtcac cagcgttacc ggatagcgca
    24301 tcttcggctt ctcgctaata aaagcatggc gcactttgcc gaccaccgct tccggtccca
    24361 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    24421 cgttgtcggt gaagcgagta cgaatgggac caggttcgat caggctgact ttaattccgc
    24481 tgtggcgcag ctccatgcgc agtgcgtctg accacgcttc cagcgcatat ttactggccg
    24541 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgaggtc atcacaatac
    24601 gcccttcacc gtgcggcaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    24661 aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa aggggaccat
    24721 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    24781 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    24841 atcccatgct gttcatgcgc tcaacatcat ccggtttccg gcaacctgcc aggacatgaa
    24901 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat accactggaa catccggtaa
    24961 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    25021 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    25081 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    25141 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    25201 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    25261 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    25321 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccagcgcgg ctgatgctgt
    25381 ttcagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct gatgctggca
    25441 ttgattaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    25501 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    25561 gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg gaaaacattg
    25621 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    25681 ccggagttga gctggttgtc aaacgtggtg agaccattgc actggtgggc gagtcgggat
    25741 cgggtaagtc aaccttgctg gcgatcctcg cagggctgga tgacggcagc agtggcgaag
    25801 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    25861 cgaagcacgt aggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    25921 aaaacgtcga gcttccggcg ctgctgcgcg gtgagagtag cgcagaaagt cgtaacgggg
    25981 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct tgatcatctt ccggcacagc
    26041 tttctggcgg cgaacagcaa cgagtggcgc tggcgcgagc ctttaatggt cgacctgatg
    26101 tgctgtttgc cgacgagccc accggcaacc ttgaccgcca gaccggcgat aaaattgccg
    26161 acctgctgtt ttccctcaac cgtgagcatg gcaccacgtt gattatggtg acccacgacc
    26221 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    26281 aagtatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    26341 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    26401 ggagaagggc ttaagccagc aaagccggga gtttatggcg ggcgatcggg cgttgcgcag
    26461 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagttgg
    26521 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    26581 caaagcggtg gatgatatct acccgatgta tggcgatctg caaaccaatc cccctggcct
    26641 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    26701 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    26761 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    26821 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gctataaatt
    26881 cggcggcagc gagaaccagc tcgacggcta tgagaaatgg ttgttacccc agcttaaacc
    26941 tgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatagaacg
    27001 ttcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcgg tggcggcggt
    27061 tgcggtggcg atgaatcatt actgtcgcag tcgctacgac ttggtggcga tcctcaagac
    27121 gctgggggca gggcgagcgc aactgcgtaa gttaatcgtc ggtcagtggt tgatggtgct
    27181 ggcgctttca gccgttaccg gtggggcgat aggcctgttg ttcgaaaacg tgttgatggt
    27241 gctgctcaag cccgttctgc ctgccgcact accgcctgcc agcctctggc cgtggctgtg
    27301 ggcgctcggc accatgacag tcatctcgct gctggtgggg ctgcgaccat accgcctgtt
    27361 gttggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg tctggccgct
    27421 gaagttttat ctgccgattg tcaccgtggt ggttgtgctg ctgctcgccg gactgatggg
    27481 tggcagtatg ctgctctggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    27541 tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    27601 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    27661 attttcgctc tcctttatgc tgctggcact gttgctggtg ttgcgtggcg atctgctcga
    27721 ccgttggcaa caacaactgc cgccagaaag cccgaactac tttttaatca acatcgctac
    27781 ggaacaggtt acgccgctga aagcgtttct cgcggaacat cagatagtcc cggaatcgtt
    27841 ttatccggtg gtgcgggcgc ggttgacggc gattaacgac aagccgacag aaggcaatgg
    27901 agatgaggcg ctcaaccgcg agctgaatct cacctggcaa aatacgcggc ctgatcataa
    27961 cccgatcgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    28021 ggcgaaacgc ttaaacgttg ccctcggcga taccgtgact tttatgggcg atacccagga
    28081 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    28141 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    28201 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    28261 cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    28321 ggcgctggaa gtgatggtgg tgctggtcac cgcctgcggt atgttgttgt tgctggcaca
    28381 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca ctctcggtgc
    28441 gggcaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgctcg ggtttgtatc
    28501 cggcctggtg gccgcaattg gtgcggaaac ggcactggcg gtactgcaat cgaaagtgtt
    28561 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcgct
    28621 actgctgtcg ctttgcggcg gctggctggg tgcgcgactg cttaagggta aggcgctgtt
    28681 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    28741 ctacgaagat cgtgcaatat attgagtttt cgaaattttg taggccggat aaggcgttca
    28801 cgccgcatcc ggcagttgtg cgccgatgcc agatgcgacg caggcgcgtc ttatcatgcc
    28861 tacgaaacag cactcacctg taggctggat aaggcgttca cgccgcatcc ggcatgaaca
    28921 gcgtgcacag aatcccccat cactgcgcct taacccattc cgccacttcc gcccactcac
    28981 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatc gggtcgtaat
    29041 attcttcaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    29101 gcgttgtcag cgccgcatcc agccttgcag ccagttcgtt atagcgttgt agtcccagcc
    29161 gacgtttaat cgccgaaagc ccgtgatgca ggtattcgca atacccctgc cagccctgtt
    29221 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcgttcaggc
    29281 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgtcgcctga gtcattcgtt
    29341 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    29401 acaggcgcaa atcctgatgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    29461 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa agcagaaccg cgatggcgcg
    29521 ccagcccttc cagatcaaca ccgttcggtt gttgttgcac taacagcgtt ttgccgcagc
    29581 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatagtc gcctgaatcg
    29641 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcgtcat
    29701 gcagccagcg ttgcacaata tgcgagcgct gaccgccacg ggcgcagcag agaataccgt
    29761 gcggattttg caggcacgct gcccgccagg cgtctatacg ctgctgacga atttcacccg
    29821 ccaccagctt atgccccagt gccagcgccg cgtctgaccc ttgctgttta tagcagatgc
    29881 caacggcggc gcgttcatcg ttattcatta acggtaggtt gatagcggcg ggcattgcgc
    29941 cttgctcaaa ctcgatgggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    30001 cacgatagtc ctgttccgtg tgtctctctt gcatagtaaa aagtgaacct caaatcagct
    30061 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    30121 gaaaatttct aaaaatccgc tgatttccgg cctgcgctgg gtaaacaagg tcacaatatc
    30181 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    30241 catcgttggg attacccggc tgaccagttg ttgattatcg agcattgact ggcaaagtga
    30301 ttttggcaaa aaaccaatgc ccacgcccgc cagatgggcg gcgattttcg tttccatatc
    30361 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaatgtacg
    30421 ggcgctgtct tcaatattga ccgccggaaa gcgccgcaac tgtgcttctg ttagcggctc
    30481 ttcaacgttc gccagcggat gatccgccgc catgacaaag cgccattgca ccgatcctaa
    30541 gggatcaaga ctaaaggtat ttgccagcgc ctcggttccc gtgacgccga tagccagtga
    30601 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    30661 ttgggtaaac gggtaacgtt catttagcca cgccagcagc ctggcaacgg cctgggggtt
    30721 atagagcagg ttgttgatga cgatattcac ctggcgttcc acgccatcat tcacctgttg
    30781 taactcgctg ggcatacttt ccagccagct cagccagtct ctggcctgac aaagtagatg
    30841 ctcgccagcc gccgtcaacg tcacgctgcg agtcgtgcgg aaaaacagcg ccactccggt
    30901 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    30961 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    31021 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    31081 gatttatcag tatttttaca tgataaccct gttcaatttg tggactaaat ctagttttgg
    31141 aaaaatattc caacttttgt gttgatgttg ttctcttaag gttttagatt gcctgttatt
    31201 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    31261 gaaacttcag gtattaccgt taagtcagga agccttctgt gcttatggcg acgtaatcga
    31321 aacgcagaaa cgggattttt tccatattaa caatggcctg gtggagcgct atcacgattt
    31381 agcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    31441 ggcgaatctg ccgctgacta ttcacgaact cgaacgtcat ccgttgggca ctcaggcctt
    31501 tatcccgatg aaaggtgaag tgtttgtggt agtcgtggcg ttaggtgacg acaaaccaga
    31561 cctgtcaacc ctgcgggcgt ttattaccaa cggcgaacag ggcgtgaatt accatcgtaa
    31621 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    31681 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    31741 acgcctgcaa ccgacttgca taagataaac gaattgttca ttgtttatgc tcatttgtag
    31801 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    31861 cctgtggcac agaagggtgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    31921 gaaaaaagtg ggggaagttc gtcggttagc gatatctctc tcaatctgga tttgccgctc
    31981 tccacgacct ttcgcttgct gaaggtttta caggcagcgg attttgtcta tcaggacagt
    32041 cagttaggct ggtggcatat aggattaggt gtctttaacg tcggtgcagc gtacatccat
    32101 aaccgcgatg tactctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    32161 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    32221 tgtaaatcga tggtcaggat gtgtgcgccg ctgggcagtc gtctgccgct gcatgcttcc
    32281 ggagcgggca aggcgctgct ttatccgctg gcggaagaag agttgatgag catcattctg
    32341 caaaccggtt tgcagcagtt tacgccaacg acgcttgtgg atatgcccac cttgctgaag
    32401 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtcggc
    32461 ctgaattgca tagcttcagc aatttacgat gatgtaggta gtgttgttgc cgctatctcc
    32521 atctccgggc cttcgtcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    32581 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    32641 tcgtatcccg ctctgcggag cgggtttttt tgacaaaatt tgagagttgg aaaatttttc
    32701 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    32761 tgctggagaa agaaggtatc accaccgcct tcggtgttcc gggagctgca atcaatccgt
    32821 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    32881 gtgcttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    32941 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    33001 attccattcc tattctgtgt attaccggcc aggctccgcg cgcccgtctg cataaagaag
    33061 atttccaggc cgttgatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    33121 ttcgtgaagc ggcgctggtg cctcgtgtgc tgcaacaggc gtttcacctg atgcgttctg
    33181 gtcgcccggg tccggtactg gtggatttac cgttcgacgt acaggttgcg gaaatcgagt
    33241 ttgatcctga tatgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    33301 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccggaggcg
    33361 gggtaattaa tgctgacgca gctgtactgt tacaacagtt tgctgaactg accagcattc
    33421 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    33481 ggatggtggg cctgcaaacc gcgcatcgtt acggtaatgc aacgttgctg gcgtccgaca
    33541 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    33601 ccgaagggcg caaaatcgtt catatcgata ttgagccgac gcaaattggc cgcgtactgt
    33661 gtccggatct tggcattgtc tctgatgcta aagcggcgct gacgctgctg gttgaagtgg
    33721 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agagtgggtc gccgactgcc
    33781 agcagcgcaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    33841 agcgcgtgta tgaagagatg aacaaagcct ttggccgcga tgtttgttat gtcaccacca
    33901 ttggcctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    33961 tcaactgtgg gcaggcaggc ccgctgggct ggacgattcc ggcggcgctg ggggtttgtg
    34021 ccgctgatcc agagcgcaat gtggtggcga tttccggtga cttcgacttc cagttcctga
    34081 ttgaagaatt agccgttggc gcacagttca acattccgta catccatgta ctggtcaata
    34141 acgcctatct gggtctgatt cgccagtcgc aacgtgcctt tgatatggat tactgcgtac
    34201 agctcgcgtt cgaaaatatt aactccagcg aagtgaacgg ttatggtgtc gaccacgtaa
    34261 aagtagcgga aggtttaggt tgtaaagcaa ttcgcgtttt caaaccggaa gacattgcac
    34321 cagcctttga acaggcaaaa gtcttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    34381 ttattctcga gcgtgtgact aatatttcga tgggcagtga actggataac gtcatggaat
    34441 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgtttc atgcactatg
    34501 aataagggag ataaataatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    34561 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    34621 tgttccctta tgactacgac attgaagaat taaaacaggt gctggcgagt aataaactcg
    34681 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgg ggtattgcct
    34741 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgttatgctc
    34801 gtgctctggg taataaaaaa attaactgtc tggtcggtaa aacgccgact ggtttcagca
    34861 gtgaacagat tcacacaacg cttgtagaaa acctgcgtta tgccgcgaat atgctgatga
    34921 aagaagatat tttattactg attgaaccta ttaaccattt tgatattcct ggtttccatc
    34981 tcaccggaac ccggcaggca ctgaaactga ttgacgatgt tggttgctgc aatttgaaaa
    35041 ttcagtatga catttatcat atgcagcgga tggaaggtga attaactaat accatgactc
    35101 agtgggccga taaaattggt cacctgcaaa ttgctgataa tccgcatcgt ggcgaaccgg
    35161 gaaccggaga aattaattac gaatatctct ttacggtaat tgaaaattct gactacaacg
    35221 gttgggttgg ctgtgaatat aaaccccaaa ccaccacgga agccggttta cgctggatgg
    35281 agccgtaccg ttaaaacgta acgctattca gacaatgctt ttttaggccg ctaagttggc
    35341 aggggatcgt gttgtctgaa ttcaggaaaa gcgaaattta aaagaggtta attatgaaac
    35401 tgggatttat tggcttaggc attatgggta caccgatggc cattaatctg gcgcgtgccg
    35461 atcatcaact gcatgtcaca accattggtc cggttgctga tgaattactg tcactgggcg
    35521 ccgttagtgt tgaaactgcg cgccaggtaa cagatgcatc ggacatcatt tttattatgg
    35581 tgccggatac accgcaggtt gaagacgttc tgtttggtga aaatggttgt accaaagcct
    35641 cgctgaaggg caaaaccatt gttgatatga gctccatttc cccgattgaa actaaacgtt
    35701 tcgctcgtca ggtgaatgaa ctgggcggcg attatctcga tgcgccagtc tccggcggtg
    35761 aaatcggcgc gcgtgaaggg acgttatcga ttatggttgg cggtgatgaa gcagtatttg
    35821 aacgtgtaaa accgctgttt gaactgctcg gtaaaaatat cactctcgtg ggcggtaacg
    35881 gcgacggtca aacctgcaaa gtggcaaatc agattatcgt ggcgctcaat attgaagcgg
    35941 tttctgaagc cttgttgttt gcttcaaaag ccggtgcgga cccggtacgt gtgcgccagg
    36001 cgctgatggg cggctttgct tcctcacgta ttctggaagt tcacggtgag cgcatgatta
    36061 aacgcacctt taatccaggt ttcaaaattg cgctgcacca gaaagatctc aacctggcac
    36121 tgcaaagtgc gaaagcactg gcgctgaacc tgccaaacac cgcgacctgc caggaattgt
    36181 ttaatacatg tgcggcaaac ggtggcagcc agttggatca ctctgcgtta gtgcaggcgc
    36241 tggaattaat ggctaaccat aaactgactt gatacccgca ataaaaatgg ccgatatcag
    36301 aaaatgaatc ggccagcaat attaaaaaaa gaaagcagcc aaa
//
LOCUS       DAZFRE010000045        36317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFRE010000045 DAZFRE010000000
VERSION     DAZFRE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.045"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..190
                     /locus_tag="ACHKUI_002551"
     CDS             <1..190
                     /locus_tag="ACHKUI_002551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598733.1"
                     /translation="SKNDKGKNSGVQKNTILCCKMNNVYLQHFLKKYVRLAEWQLRKY
                     DFLLSECSASHYYLTQYS"
     gene            211..1011
                     /gene="ydcF"
                     /locus_tag="ACHKUI_002552"
     CDS             211..1011
                     /gene="ydcF"
                     /locus_tag="ACHKUI_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6598734.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1208..2647
                     /gene="aldA"
                     /locus_tag="ACHKUI_002553"
     CDS             1208..2647
                     /gene="aldA"
                     /locus_tag="ACHKUI_002553"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6598735.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEVVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2689..3690)
                     /gene="gap"
                     /locus_tag="ACHKUI_002554"
     CDS             complement(2689..3690)
                     /gene="gap"
                     /locus_tag="ACHKUI_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6598736.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3879..4409
                     /gene="cybB"
                     /locus_tag="ACHKUI_002555"
     CDS             3879..4409
                     /gene="cybB"
                     /locus_tag="ACHKUI_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6598737.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKCPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4654..4827
                     /locus_tag="ACHKUI_002556"
     CDS             4654..4827
                     /locus_tag="ACHKUI_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598738.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4853..4906
                     /locus_tag="ACHKUI_002557"
     CDS             4853..4906
                     /locus_tag="ACHKUI_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6598739.1"
                     /translation="MNLLVRCAGKIPALALT"
     gene            complement(4899..5048)
                     /gene="hokB"
                     /locus_tag="ACHKUI_002558"
     CDS             complement(4899..5048)
                     /gene="hokB"
                     /locus_tag="ACHKUI_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO6598740.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5438..7077
                     /gene="trg"
                     /locus_tag="ACHKUI_002559"
                     /pseudo
     CDS             5438..7077
                     /gene="trg"
                     /locus_tag="ACHKUI_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(7115..8038)
                     /gene="ydcI"
                     /locus_tag="ACHKUI_002560"
     CDS             complement(7115..8038)
                     /gene="ydcI"
                     /locus_tag="ACHKUI_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6598741.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            8255..9598
                     /gene="ydcJ"
                     /locus_tag="ACHKUI_002561"
     CDS             8255..9598
                     /gene="ydcJ"
                     /locus_tag="ACHKUI_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6598742.1"
                     /translation="MANSITADEIREQFSQAMSVMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRSAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGGPVLDEFQLYQEAEERSKRRCGLL"
     gene            9823..11478
                     /gene="opgD"
                     /locus_tag="ACHKUI_002562"
                     /gene_synonym="mdoD"
     CDS             9823..11478
                     /gene="opgD"
                     /locus_tag="ACHKUI_002562"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6598743.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            11618..11842
                     /gene="ydcH"
                     /locus_tag="ACHKUI_002563"
     CDS             11618..11842
                     /gene="ydcH"
                     /locus_tag="ACHKUI_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6598744.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            11905..12444
                     /gene="rimL"
                     /locus_tag="ACHKUI_002564"
     CDS             11905..12444
                     /gene="rimL"
                     /locus_tag="ACHKUI_002564"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO6598745.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(12436..13416)
                     /gene="ydcK"
                     /locus_tag="ACHKUI_002565"
     CDS             complement(12436..13416)
                     /gene="ydcK"
                     /locus_tag="ACHKUI_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6598746.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTPLAGLL"
     gene            13540..14532
                     /gene="tehA"
                     /locus_tag="ACHKUI_002566"
     CDS             13540..14532
                     /gene="tehA"
                     /locus_tag="ACHKUI_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6598747.1"
                     /translation="MQSDKGLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            14529..15122
                     /gene="tehB"
                     /locus_tag="ACHKUI_002567"
     CDS             14529..15122
                     /gene="tehB"
                     /locus_tag="ACHKUI_002567"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6598748.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNTMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            15425..16093
                     /gene="ydcL"
                     /locus_tag="ACHKUI_002568"
     CDS             15425..16093
                     /gene="ydcL"
                     /locus_tag="ACHKUI_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6598749.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYLVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(16128..17318)
                     /gene="ydcO"
                     /locus_tag="ACHKUI_002569"
     CDS             complement(16128..17318)
                     /gene="ydcO"
                     /locus_tag="ACHKUI_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6598750.1"
                     /translation="MTHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGA
                     TTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGV
                     FIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSM
                     LLAWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSL
                     SVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAI
                     TAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLAL
                     LSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIA
                     DRNR"
     gene            17392..17928
                     /gene="sutR"
                     /locus_tag="ACHKUI_002570"
     CDS             17392..17928
                     /gene="sutR"
                     /locus_tag="ACHKUI_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6598751.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            18001..19962
                     /gene="rlhA"
                     /locus_tag="ACHKUI_002571"
     CDS             18001..19962
                     /gene="rlhA"
                     /locus_tag="ACHKUI_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO6598752.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(20054..20284)
                     /gene="yncJ"
                     /locus_tag="ACHKUI_002572"
     CDS             complement(20054..20284)
                     /gene="yncJ"
                     /locus_tag="ACHKUI_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO6598753.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            20463..20699
                     /gene="hicB"
                     /locus_tag="ACHKUI_002573"
                     /pseudo
     CDS             20463..20699
                     /gene="hicB"
                     /locus_tag="ACHKUI_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            20778..22184
                     /gene="ydcR"
                     /locus_tag="ACHKUI_002574"
     CDS             20778..22184
                     /gene="ydcR"
                     /locus_tag="ACHKUI_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6598754.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            22429..23574
                     /gene="ydcS"
                     /locus_tag="ACHKUI_002575"
     CDS             22429..23574
                     /gene="ydcS"
                     /locus_tag="ACHKUI_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO6598755.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            23592..24605
                     /gene="ydcT"
                     /locus_tag="ACHKUI_002576"
     CDS             23592..24605
                     /gene="ydcT"
                     /locus_tag="ACHKUI_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6598756.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            24606..25547
                     /gene="ydcU"
                     /locus_tag="ACHKUI_002577"
     CDS             24606..25547
                     /gene="ydcU"
                     /locus_tag="ACHKUI_002577"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598757.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            25537..26331
                     /gene="ydcV"
                     /locus_tag="ACHKUI_002578"
     CDS             25537..26331
                     /gene="ydcV"
                     /locus_tag="ACHKUI_002578"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598758.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            26353..27777
                     /gene="patD"
                     /locus_tag="ACHKUI_002579"
     CDS             26353..27777
                     /gene="patD"
                     /locus_tag="ACHKUI_002579"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6598759.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCPNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEVVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(27874..27969)
                     /gene="yncL"
                     /locus_tag="ACHKUI_002580"
     CDS             complement(27874..27969)
                     /gene="yncL"
                     /locus_tag="ACHKUI_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6598760.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            28164..28337
                     /gene="ortT"
                     /locus_tag="ACHKUI_002581"
     CDS             28164..28337
                     /gene="ortT"
                     /locus_tag="ACHKUI_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6598761.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            28423..28656
                     /gene="ydcY"
                     /locus_tag="ACHKUI_002582"
     CDS             28423..28656
                     /gene="ydcY"
                     /locus_tag="ACHKUI_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6598762.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(28657..29106)
                     /gene="ydcZ"
                     /locus_tag="ACHKUI_002583"
     CDS             complement(28657..29106)
                     /gene="ydcZ"
                     /locus_tag="ACHKUI_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6598763.1"
                     /translation="MSQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(29103..29621)
                     /gene="mddA"
                     /locus_tag="ACHKUI_002584"
     CDS             complement(29103..29621)
                     /gene="mddA"
                     /locus_tag="ACHKUI_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6598764.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            29802..29870
                     /locus_tag="ACHKUI_002585"
                     /pseudo
     CDS             29802..29870
                     /locus_tag="ACHKUI_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29870..30025
                     /locus_tag="ACHKUI_002586"
                     /pseudo
     CDS             29870..30025
                     /locus_tag="ACHKUI_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(30102..32204)
                     /gene="pqqU"
                     /locus_tag="ACHKUI_002587"
     CDS             complement(30102..32204)
                     /gene="pqqU"
                     /locus_tag="ACHKUI_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6598765.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNSDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRDFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGQNYGVGINIAWRFE"
     gene            32446..33507
                     /gene="yncE"
                     /locus_tag="ACHKUI_002588"
     CDS             32446..33507
                     /gene="yncE"
                     /locus_tag="ACHKUI_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6598766.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTNNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(33620..35119)
                     /gene="ansP"
                     /locus_tag="ACHKUI_002589"
     CDS             complement(33620..35119)
                     /gene="ansP"
                     /locus_tag="ACHKUI_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6598767.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSS"
     gene            35386..35986
                     /gene="yncG"
                     /locus_tag="ACHKUI_002590"
                     /pseudo
     CDS             35386..35986
                     /gene="yncG"
                     /locus_tag="ACHKUI_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
BASE COUNT         9244 a         8777 c         9224 g         9072 t
ORIGIN      
        1 ttctaaaaac gataagggaa aaaattcagg agttcagaaa aatacaattc tctgctgcaa
       61 gatgaataat gtttatctac agcatttcct taaaaaatat gtcaggcttg cggagtggca
      121 gttaaggaaa tacgacttcc tcctttcaga gtgctccgct tctcactatt atctcacgca
      181 gtattcttaa gggaacgata aggaggaacc atgaatatta ccccgtttcc gacgctttcg
      241 ccggcaacca tagatgccat aaatgttatc ggacagtggc tggcgcagga tgatttctcc
      301 ggtgaggtgc cgtatcaggc cgattgcgtg atccttgcag gcaatgcggt tatgccgact
      361 atcgatgcgg catgtaagat tgcccgcgat cagcaaattc ctttactgat tagtggtggt
      421 atcggtcact cgacaacttt tttgtatagc gccatcgcac agcatccaca ctacaacact
      481 atccgcacca ctggcagagc tgaagctacc atcctggcgg atatcgctca tcagttctgg
      541 catattccgc atgaaaaaat ctggattgaa gaccagtcaa caaactgcgg tgaaaacgca
      601 cgctttagca tcgcgctatt gaatcaggcc gtagaacgag ttcatacggc tatcgttgtt
      661 caggacccca ccatgcagcg gcgcacgatg gcgacgttcc gccgtataac cggggataat
      721 cccgatgcac cacgctggtt aagttatccc ggattcgttc ctcagttagg aaataacgca
      781 gacagtgtaa tctttgttaa tccgttgcaa ggattatggc cggttgagcg ttatctatcg
      841 ctactcactg gcgagctgcc gcgtttacgc gatgatagcg atggctacgg cccccgcggg
      901 cgagatttta tcgttcacgt tgattttccg gcagaagtca tccaggcatg gcaaacgctg
      961 aaacacgatg cggtgctcat cgaggcgatg gaaagtcgct cgttacgtta aaaattgccc
     1021 gttcgtgaac caattgtttg caaacgggca tgacttctga cttttatttc tgcctttaat
     1081 tccttttaca cttgttttta tgaagccctt cacagaattg tcctttcacg atgccgtctc
     1141 tctgataatt gatgttaatt aacaataaat ttaccaaaaa caaacatgca aatcacagga
     1201 gtcgcccatg tcagtacccg ttcaacatcc tatgtatatc gatggacagt ttgttacctg
     1261 gcgtggagac gcatggattg atgtggtaaa ccctgctaca gaggttgtca tttcccgcat
     1321 tcccgatggt caggccgagg atgcccgtaa ggcaatcgat gcagcagaac gtgcacaacc
     1381 agaatgggaa gcgttgcctg ctattgaacg cgccagttgg ttgcgcaaaa tctccgccgg
     1441 gatccgggaa cgtgccagtg aaatcagtgc gctgattgtc gaagaagggg gcaagatcca
     1501 gcagttggct gaagtcgaag tggcttttac tgcggactat atcgattata tggcggagtg
     1561 ggcacggcgt tacgagggcg agattattca aagcgatcgt ccaggagaaa atatccttct
     1621 gtttaaacgt gcgcttggcg tgactaccgg cattctgccg tggaactttc cgttcttcct
     1681 cattgcccgc aaaatggctc ccgctctttt gaccggtaat accatcgtca ttaaacccag
     1741 tgaatttacg ccaaacaatg cgattgcatt cgccaaaatc gttgatgaaa taggccttcc
     1801 gcgcggcgtg tttaaccttg tactcgggcg tggtgaaacc gttgggcaag aactggcggg
     1861 taacccaaag gtcgcaatgg tcagtatgac aggcagcgtc tctgcaggtg aaaagattat
     1921 ggcgactgcg gcgaaaaaca tcaccaaagt gtgcctggaa ttggggggta aagcaccggc
     1981 tatcgtaatg gacgatgccg atcttgaact ggcagtcaaa gccatcgttg attcacgcgt
     2041 cattaatagt gggcaagtgt gtaactgtgc agaacgtgtt tatgtacaga aaagcattta
     2101 tgatcagttc gtcaatcggc tgggggaagc gatgcaggcg gttcaatttg gtaaccccgc
     2161 tgaacgcaac gacattgcga tgggaccgtt gattaacgcc gcggcgctgg aaagggttga
     2221 gcaaaaagtg gcgcgcgcag tagaagaagg ggcgagagtg gcgttgggtg gcaaagcggt
     2281 agaggggaaa ggatattatt atccgccgac attgctgctg gatgttcgcc aggaaatgtc
     2341 gattatgcat gaggaaacct ttggcccggt tctgccggtg gtcgcatttg acacgctgga
     2401 agaggctatc tcaatggcta atgacagtga ttacggcctg acctcatcaa tctataccca
     2461 aaatctgaac gtcgcgatga aagccattaa agggctgaag tttggtgaaa cttacatcaa
     2521 ccgtgaaaac ttcgaagcta tgcaaggctt ccacgctgga tggcgtaaat ccggtattgg
     2581 cggcgcagat ggtaaacatg gcttgcatga atatctgcag acccaggtgg tttatttaca
     2641 gtcgtaatga gtgaaagagg cggaggtttt ttcctccgcc tgtgcgcgtc agagtttagc
     2701 gaatttttcg agggtgcgaa taagctgcgt gacgaagcca tattcgttat cgtaccaggc
     2761 gaccgttttc accagttgta aatcgcccac ggcggtaatt tccgtttgcg tggcatcaaa
     2821 caccgaaccg aaatggctgc caatgatatc ggaagagact atttcttcat cagtataacc
     2881 aaatgactcg ttattgtttg tcgccttttt aagcgcgttg ttcacctctt cggcagtcac
     2941 tttctttccg agaatcgaca ccagttcagt gaccgaacct gttttcaccg gcacgcgttg
     3001 cgcatgacct ttcagtttgc cgctcagttc cgggatcacc agaccaatgg cttttgccgc
     3061 ccccgtagtg tggggaatga tattttctgc cgctgcgcgt gaagcacgta aatctttacc
     3121 acgtgggcca tccaccagtg actgggtgcc ggtataggca tgaatggttg tcatcgtgcc
     3181 gacttctatc ccgaaactgt catgcaaggc tttggccatc ggcgcaaggc agttagtggt
     3241 gcatgacgcc acggaaacaa tggtgtcgtt gccatccaga gtgtcgtcat tgacgttata
     3301 aacgatggtt ttcatttcac cggcaggggc ggaaatcaac accttcttcg cgccagcatc
     3361 aagatgcgcc tgcgatttct cggcggaggt ataaaagcct gtacattcga caatgatttc
     3421 tgcgcctttc gctttccacg gaatattttt agcctctttt tcggcgtaaa ccgcgatact
     3481 tttcccatca acgataagtg aatcttccgt ataatcaacg ctccagggga acggtccgta
     3541 gtttgaatca tgtttcagca ggtaggcgag aatttttggg gaagtgagat cattaatagc
     3601 gacaacgtct atgttgcttt tgacttcaag taatcgacgc aacaccagtc gaccgatacg
     3661 accaaaacca ttaataccaa ctttactcat ggtcttctcc tgtcaggaac gttcggatga
     3721 aaattgatcc tttccaggct tagaccagga tggcgggatg ggcaatctac attcttgcag
     3781 tgaaacgtaa cgtactgaaa acgggtgaac aatatttaat gaaattttaa gaaaagcccg
     3841 ttatgttaac ggcaaattat gttaaagcag gaaatgttat gggaaataaa tactcaaggt
     3901 tacaaatcag cattcactgg ctggtctttt tgctggttat cgtagcgtat tgcgcaatgg
     3961 agtttcgtgg tttcttccca cgtagcgatc ggcccctcat caacatgatt catgtttcct
     4021 gtggcatctc aattctcgta ctgatggtgg ttcgtctttt gttaaggctg aaatgcccaa
     4081 ccccgccgat tatacctaag ccaaaaccga tgatgacggg actggcgcat ttgggccatt
     4141 tggtgattta tctcctgttt attgcgctgc cagtgattgg tttggtgatg atgtataacc
     4201 ggggcaaccc gtggtttgcg tttggtttga cgatgcctta cgcttcagag gccaatttcg
     4261 agcgggttga tagcttaaag tcgtggcatg aaacgctggc gaatctggga tattttgtca
     4321 tcgggttgca cgctgcggct gcactggcac accactattt ctggaaggac aacacacttc
     4381 tacgcatgat gccgcgtaaa cgttcctgaa ggatatttaa agaaaacgcc tgtactaaaa
     4441 ccgacccgtg gtacaggcga agaatacggg cctacatcgg aagcgcctat tatatttatt
     4501 tgtatgacaa ataaaaccat aattcttgtc tatacgtcca tctggcttat ctttaatcaa
     4561 ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata acgttcaaat
     4621 cgaacataag attcgataaa tcatggaggt tatatgaaaa aactggcact tatcctggtt
     4681 atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc tggttcgaaa
     4741 ggggggatct cacactgtac ggcaggcggc aaatttgtct gtaatgatgg ttctattagt
     4801 gcatcgaaaa aaacatgcac taactgaagt gtaaaagggg tgccatgaga aaatgaatct
     4861 gctagtcaga tgcgcgggga aaatccccgc gcttgccctt acctagacgt gcaggccatg
     4921 agcgcagcaa cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc
     4981 aggagtgtga atgtcagaat cgtaatgcag ataatgagca gacacaccac cagagggttg
     5041 cgcttcatag cctttctcct tgccggatgg cgggtaagag gctaacatct gaattgcttg
     5101 gttcattcgt tggcctcggt tgatagaaat atcggtcggg gccttcgtct ttctgattcc
     5161 cggttagcct gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag
     5221 ggacgaaaat aatcacttga cgaaattgcg tgctgttttc cagaattttt cgtcattcgg
     5281 tttagccgtt cgttactctc ttcattccaa tggcattatt tttctctgcc ataattgttg
     5341 taaaaatgtg acgcaaagag gtttttggtc ataagtaatt accgtcaagt gccgatgact
     5401 ttctatcagg agtaaacctg gacgagagac aacggtaatg aatacaactc cctcacagcg
     5461 attaggtttt ttgcatcaca tcaggctggt tccgttattt gcctgcattc ttggcggtat
     5521 cttagttcta ttcgcattaa gttctgctct ggctggctat ttcctctggc aggccgatcg
     5581 cgatcagcgt gatgttactg cggagattga gattcggacc ggttagcgaa cagttcagat
     5641 tttttgcgtt cagcccggat caatatgatt caggccgggg cagcgagtcg tattgcggaa
     5701 atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa
     5761 ggttatcgtg cttatcagaa tcgatcggtg aaaacacctg ctgatgaagc cctcgacact
     5821 gaattaaatc aacgcttcca ggcttatatc acgggtatgc aacccatgct gaaatatgcc
     5881 aaaaatggca tgtttgaggc gattatcaat catgaaagtg agcagatccg accgctggat
     5941 aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa
     6001 ctggcggaac aggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt
     6061 gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt
     6121 ccactgcaac atgccgcaca acggattgaa aaaattgcca gtggcgatct gacgatgaat
     6181 gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag
     6241 cattcactgg ggatgacagt agggactgtt cgacagggtg cggaagagat ttatcgtggc
     6301 accagcgaaa tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg
     6361 gctatcgaac aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg
     6421 gataacgcgc atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc
     6481 gggcagatgg tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa
     6541 atttctgaga tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg
     6601 aatgctgccg ttgaagccgc gcgagcgggt gagcaaggcc ctggatttgc cgttgtcgcc
     6661 agcgaagtac ggacactcgc aagccgcagc gctcaggcgg cgaaagagat tgaaggcttg
     6721 atcagtgaat cagtcaggtt aattgacctg ggagcggatg aggtggccac ggccgggaaa
     6781 accatgagca ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc
     6841 gccgcctcgg atgaacaaag cagaggcata acacaggtta gccaggcgat ttctgaaatg
     6901 gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcagcggc ggcggtgtcc
     6961 cttgaagaac aggcggcacg attaactgag gcggtggacg tattccgtct gaacaaacat
     7021 tctgtgtcgg cagaacctcg cggagctggt gaaccagtta gtttcgctac agtgtgaaaa
     7081 tgttaaagga gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag
     7141 cgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa
     7201 tcggttcgcc tgcaccatgt cccggaacag gcagggctac cagcgtggca tgacgcaggt
     7261 cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacc gtaagttgac
     7321 gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacat ccctggctct
     7381 gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc
     7441 attccagcac ccggcttagc gttacgttct cctgaagtag cgggtgattg gggcggacaa
     7501 ccagcttcag cgattcaaga aacagcagtt cataattaag cccgttcatc agttcaggat
     7561 ctgacatccg accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag
     7621 ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta
     7681 taaccgaagg taatatccct agtgccgcag taggtagcgc accaaccctg acgacatcat
     7741 tattaagacc ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc
     7801 tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac
     7861 gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt caatgtctta gagagcgcag
     7921 gttgactcaa attaagggtt tcagccgcgc gccccaaagt tccttgttgt gcgacagcta
     7981 cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa
     8041 gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgttttatta tgataacttg
     8101 attgcaaaat attattaaca attaaagcaa ctatgttaca gcaaaatgga taatattgat
     8161 gttttcgcgg cgagatcaca gtttgtaaat tcttccagcc ggagtgaatg cggttaccta
     8221 cactccagat tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga
     8281 gattcgggaa cagttttcgc aggcaatgtc agtcatgtac cagcaagaag ttccgcagta
     8341 cggcacgctg ctggaactgg tagctgatgt aaatctggcg gtgctggaaa acaatcctca
     8401 actgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg
     8461 ggcgattcgc gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat
     8521 ggggatgtac ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac
     8581 agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacctc
     8641 gttactccgt cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct
     8701 gcgtcagcgc gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca
     8761 gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg
     8821 ctggcaccag tcagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg
     8881 gttgattgct gatgtggtct gttttcctgg atgccatatc aaccatctga cgccacgtac
     8941 gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaagttct
     9001 gatcgaaggg ccgccgcgcc gcgaggtgcc gattttacta cgccagacca gctttaaagc
     9061 actggaagag acggtgttgt ttgcggggca gaaacagggc acacataccg cgcgctttgg
     9121 tgaaattgag cagcgtggtg tggcattaac gccgaaaggg cgacaactgt atgatgatct
     9181 tctgcggagc gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac
     9241 cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggctgg gatggttccg
     9301 gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg attcatcccg gagacgatcc
     9361 acagccctta atagaacgtg gttgggtagt ggcgcaaccc attacctatg aagatttctt
     9421 gcccgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc aggcatgcaa
     9481 tcacggtgat gccagtcgcg aagcatttga gcaggcgttg gggggtccgg ttttggatga
     9541 gttccagctt tatcaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat
     9601 tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca
     9661 tcgacataaa aaaaatgcca atttatgcat attctctgat ttcaacaatt ggattattaa
     9721 taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaatgacca
     9781 gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt
     9841 attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt
     9901 tctcaggcgg cattcgcggc cgattctgat attgccgacg ggcaaaccca gcgttttgac
     9961 ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcatggcg tggtgcacca
    10021 cgtccgttac ctgatactct ggcgacaatg acgccgcagg cttataacag tattcaatac
    10081 gacgccgaaa aatcgctctg gcataacgtt gaaaaccgtc aactggacgc tcagtttttc
    10141 catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat
    10201 ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc gggtgtggat
    10261 accaaacaat tagaagggca aagcgatctc ggttttgccg gttttcgcgt gtttaaagcc
    10321 cccgaactgg cgcgccgtga tgtagtatca ttcctcggcg cgagttattt ccgcgccgtt
    10381 gatgacacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac
    10441 agcaaagaag agttccccga ctttaccgcc ttctggtttg atacggtaaa accaggggca
    10501 accaccttta ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc
    10561 actatccatt gtgagaaaaa tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc
    10621 aaagacatta aacagctggg cattgcaccg atgaccagta tgttcagctg cggtactaat
    10681 gaacgtcgga tgtgcgacac cattcatccg caaatccatg actctgatcg tctttccatg
    10741 tggcggggca acggcgagtg gatttgccgt ccgctgaaca atccgcaaaa attgcagttc
    10801 aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgatttc
    10861 tcccattatc aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg
    10921 cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg
    10981 ctggataaca ttgtctgctt ctggcagcca gaaaaagcgg taaaagcggg tgatgagttt
    11041 gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc
    11101 gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac
    11161 tatcccgaaa aatgggcgcg acgttttgcc gtcgatttcg ttggtggtga tctgaaagct
    11221 gccgcgccaa aaggcattga gccggtgatt acgctttcca gtggggaagc gaaacaaatc
    11281 gaaattctct atattgaacc cattgatggt tatcgtattc agtttgactg gtatccgact
    11341 tcggactcca ctgatccggt cgatatgcgg atgtatctgc gttgtcaggg cgacgctatc
    11401 agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgat
    11461 gaccgcgtga tgagttaatc gttttttctt cggcatcttc ttcgggaggt gccgtctggt
    11521 taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt
    11581 tcagatacac cttgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt
    11641 aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa
    11701 acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt
    11761 ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca
    11821 ggtgagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacag
    11881 ctgcatggat tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa
    11941 ttacatgctg ttgcggaaag tcatgtcaca cctctttatc agttaatctg taaaaataaa
    12001 acctggttac agcagtcgct aaactggccg cagtttgttc aaagtgaaga ggacacgcga
    12061 aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt
    12121 ttcaaagaag atgaacttat cggcgttatc tcttttaatc gtattgaacc actgaataaa
    12181 accgctgaaa tcggctactg gctcgacgaa tctcatcagg ggcaggggat catttctcag
    12241 gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc
    12301 aaatgtcggg tggacaatgc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt
    12361 gaaggttgcc tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac
    12421 gcgcgtatta tcgattcaca ataaccctgc caacggcgtt cgagtaatgc gctcctcgcc
    12481 gttaatgact tttggaccac ggacatgaat cgtgtcgcca tcaaaggctt caacgacggc
    12541 gtggtcggtt aactccacat ggttttcaat tatcaccgca ccagtaatac ggctctcacc
    12601 ttgtatgaca acgtgctcgt cgagcagaat cggtccgcca cgtaccacgg catttccgcc
    12661 aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg
    12721 tgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgtgcgtg
    12781 accgtaaact ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaatact
    12841 ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc
    12901 ataaatttgt gcctggtgga caatacgcga ggcgctaacc ctcgcgcgat cataaatttg
    12961 tagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc
    13021 aattaaggcg tgaccaaata ttctacattg accgcaaact aatgagtcac taatggtgac
    13081 gctgtcactt atatatgccc cctggctgat ttcactatta tcgatccaga cattatccgt
    13141 tgcgtaaacc tctccccaca gcacgctggt gccggtgata cgcgtattcc cggaaatcac
    13201 cgttccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa
    13261 caccgtttcg cgatcgatcc agccgccagc gcttcctgct atcacatcgt taaaatcgct
    13321 catggcgata atttgccgta ataacacact ttttttagtg ccatcttcct gataactaaa
    13381 ggctcgctgt tcttcactta gacgatattt acgcatagcg ttttcccaca ggtgacttac
    13441 tataaccgta gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt
    13501 tcaaatatac tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagggctc
    13561 aatttgccgg caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg
    13621 cgctatgcca gccaggtttg gcaggtcagc cactggttgg gggatgggct ggtgattctg
    13681 gcgatgatca tctggggatt attgactggc gcatttatta cccgactcat acgctttccg
    13741 catagcgtgc tggcagaagt gcgccatcca gtgctgagca gttttgtgag tttgtttccg
    13801 gcaacgacga tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc
    13861 ctgttcagtt ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta
    13921 tggcgcggat ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc
    13981 aacaacttta tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg
    14041 tttttaggcg caggcgtttt ctcatggcta agcctggaac cggtgatctt gcagcgtctg
    14101 cgcagttcgg gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct
    14161 gcgctggtgg cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg
    14221 aaaatgcttt tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg
    14281 tatctctccc agccatttaa tgcttcattc tggagtttct cgttcggtgt atctgcactg
    14341 gcaaccaccg gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg
    14401 gtgccgctgt ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg
    14461 cttctgatgc agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag
    14521 gacaaagaat gatcattcgt gatgaaaact attttactga taaatatgaa ttaacccgca
    14581 cacactctga agtgctggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg
    14641 gctgtggcaa tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat
    14701 gggataaaaa taccatgagt atcgccaacg tcgagcgcat taagtccatt gaaaatctgg
    14761 ataatttaca cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt
    14821 ttattctttc gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggctgattg
    14881 ccaatatgca acgttgcact aaacctggcg gttacaacct gattgtggcg gcgatggata
    14941 ccgctgatta tccatgtacc gtcggcttcc cgtttgcctt caaagagggg gaattacgtc
    15001 gatattacga aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca
    15061 ccgacgccaa cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat
    15121 gatccggtaa gaacaaagcg cgtgaaaatt ccccacgctg agatggttta ctgttcttct
    15181 tttcggtaag catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt
    15241 ttagccgctt aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca
    15301 ttgactatgt cgtctgaaaa ggggctaaaa aatttatttt caccaacact ttttttgcca
    15361 caacacgaag cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact
    15421 attcatgcgt accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc
    15481 tggttgtgcc tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc
    15541 tgatttaaaa gaaacaacct cggcaacagg taaacctgtt ttacgttggg tagacccgag
    15601 ttttgatcaa agcaaatatg acagcatcgt ctggaaccca atcacttatt atctggtacc
    15661 gaaaccttct acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac
    15721 cgaaatgaaa gaagcgatag cgcagcgtaa accactggtt accaccgctg ggccgcgtag
    15781 tctgattttc cgtggggcca ttaccggtgt agataccagt aaagaagggc tgcaattcta
    15841 tgaagtggtt cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac
    15901 catggatact cgcttgtatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt
    15961 tatcaaggtc gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc
    16021 tttcgaaaat attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt
    16081 taacaaaaag tagttccaga cgcgccatcg ttagatggcg ctttttatta tcggtttctg
    16141 tcagcgatta aattcaacac cacgtaacaa acgcctccgg caattaatcc ccaaaacgca
    16201 gaaccaatcc cgaacagcgt caatccactt gccgttacca gaaatgccac caccgccgcg
    16261 tcgcgctcac gctcattatg cagcgcctga tacaaactgc cgccaatggt actcaacagc
    16321 gcaagacctg ccagcatctg gatccaactt acgggcaggg cagccatcat cccggtaatg
    16381 gcactaccaa acagacctgc gatcaaataa aaaatgccgg caacggcagc ggccagccaa
    16441 cgttgatctt tatccggatg cgcttccggg ctttggcaaa tagccgcggt gattgccgca
    16501 ataccgacgg aataaacgcc gaaaggggaa aaaacaagcg ccagcaatcc agtaaacaca
    16561 atcaacggcg aaacaggagc cgaatacccg gctgctttca ttgctgcgat acccggtgcg
    16621 ttttgcgatg ccatcgtcac cagaaaaagg gggagtgcaa cgctcagact gtgagcaaac
    16681 gaaaaatcag ggggaatata agtggggaga acgggtttaa agacaacatc agttgtgaca
    16741 acgtcacctt gcgcgataac gatcacgatc ccaataatca tcgcggcaat taccgcatag
    16801 cgcggcgcaa cggccctggt tgccagccat gccagcaaca tactgccaca caacgtaaat
    16861 tgaccatcca gactggcaaa cgcctgtaaa ccaaaacgta ataaaatccc ggcaagcatt
    16921 gccgccgcaa gcgagtgcgg aataatgcgc atcagacgag caaagagtcc cgttatgccg
    16981 cagaggacta ttaacacgtt ggtgacaata aaaacgccga tggcttcgtt aagtgttagt
    17041 ccctgtaatc cggtgaccaa caaagccgcg ccaggcgttg accatgcggt gagaacaggt
    17101 acgcgatacc ataatgtcag agtcagcgta ctgacgccca ttgccagccc cagcgccgtc
    17161 atccagccag agatttgtgc agtggtggct ccggcgacaa tcgctgcttg ccagattatt
    17221 gccgctgaac tggcgtagcc aattaatacc gccagaaacc ccgccagtag cgtgggtgga
    17281 ggaatagaaa acagacgcat agtcacctcg tgcgttataa cgtacggcta acgtagcatt
    17341 tgtccgttat aacgcacaag tgataaactt ccgttttgcc ggaggagtcg catggaaaat
    17401 ctcgctcgct ttttatccac cacacttaaa caactacgtc agcagcgcgg ctggagtctt
    17461 tcgcgactgg cagaagcgac gggcgtttct aaagcgatgt tagggcagat tgagcgtaat
    17521 gaatccagcc cgacggtcgc gacattatgg aaaattgcca ccgggttgaa cgtgccattt
    17581 tcaacattta tttctccgcc gcagtcctct acaccttcag tttatgatcc acaacggcag
    17641 gcgatggtga tcacttcgct gtttccttat gatccacaac tctgctttga acacttctcg
    17701 attcagatgg cacccggtgc aataagcgaa tcgacgcccc atgaaaaggg ggtaatcgaa
    17761 catgtcttcg tcattgatgg acaacttgat ctgtgtgttg atggtgaatg gcagagcctt
    17821 aattgtgggg aaggcgttcg atttgctgca gacgtcacgc atgtctatcg taatggtggg
    17881 gagcaaaccg tacattttca ttccctcatc cattacccgc gcagttaagc gggaaaacta
    17941 tttcgcaacg tcgcgcttgt ggctaaaata gccgccattt ttcagctact ggataagaaa
    18001 gtgaccgtat cttctcatcg tcttgaactg ttaagcccgg cacgcgatgc cgccattgcc
    18061 cgcgaagcta ttttgcacgg tgccgatgct gtttatatcg gcggccctgg ttttggtgcc
    18121 cgtcataatg ccagtaatag cctgaaagat attgccgagt tggtgccgtt tgcccatcgt
    18181 tatggtgcaa aaattttcgt cacgcttaac accattttgc atgatgatga gctggaaccc
    18241 gcgcaacggc tgattactga cctctaccag accggtgtcg atgcgctgat tgttcaggat
    18301 atggggattc tggaacttga tattccgccg attgaactgc acgctagtac gcagtgcgat
    18361 attcgtacag tagaaaaagc gaagttcctc tctgatgttg gcttcacgca gattgtgctg
    18421 gcgcgagagc tgaatcttga gcagatccgc gcgattcacc aggctacgga cgcgaccatt
    18481 gaattcttta ttcatggcgc attgtgcgtg gcctattcgg gtcagtgcta catttctcat
    18541 gcgcaaacag ggcgtagcgc caaccgtggc gattgctcgc aggcgtgccg tttgccatac
    18601 acattgaaag acgatcaggg gcgtgtggtt tcctatgaaa aacatctgct gtcgatgaaa
    18661 gataacgatc agactgccaa cctcggcgcg ctgattgatg ctggcgtacg ctccttcaag
    18721 attgaagggc gttacaaaga tatgagctac gtgaagaata tcaccgccca ttatcgtcag
    18781 atgctggatg ccattattga agaacgtggc gatctggcgc gcgcttcatc aggtcgtact
    18841 gaacatttct ttgttccatc gacggaaaag actttccacc gtggtagcac agattatttt
    18901 gtgaatgccc gtaaaggcga tattggcgcg ttcgattcgc cgaaatttat cggcctgccg
    18961 gtgggcgaag tattgaaagt agcgaaagat catctcgatg ttgccgttac cgagccactg
    19021 gcaaatggcg atggcctgaa cgtgatgatt aaacgtgaag tcgtcggttt tcgtgccaat
    19081 acggtagaga aaaccggaga aaatcagtac cgcgtctggc ccaatgaaat gccagcagat
    19141 ttgcacaaaa ttcgcccgca tcacccacta aaccgaaatc ttgatcataa ctggcagcag
    19201 gcactgacaa aaacctccag cgaacgccgg gtggcggtag acattgaact gggcggctgg
    19261 caggaacaac tgattctgac cctcaccagt gaagagggtg tcagcatcac acatacgctg
    19321 gacgggcagt tcgacgaagc caataacgcc gaaaaagcaa tgaacaatct gaaggatggt
    19381 ctggcaaaac tggggcaaac catctattac gcccgcaatg tgcaaattaa tttgcctggc
    19441 gcgctgtttg taccaaacag tctgttaaac cagttccgcc gtgaagccgc cgatatgctt
    19501 gatgctgcgc gtcttgccag ttaccagcgc ggcagccgta aaccggttgc tgatcctgcg
    19561 ccggtttatc cgcaaacgca tctgagtttc ctcgcgaacg tatacaacca gaaagcgcgt
    19621 gaattttatc atcgctatgg tgtgcaactg attgacgcgg cgtatgaggc acatgaagag
    19681 aagggcgaag tcccggtgat gatcaccaag cattgtctgc gctttgcctt caatctgtgc
    19741 ccgaaacagg cgaaaggcaa tatcaaaagc tggaaggcga cgccaatgca actggttaac
    19801 ggcgatgaag tattaacgct aaagtttgat tgccgcccat gcgagatgca cgtcattggc
    19861 aaaatcaaaa atcacatact gaaaatgccg ttaccgggaa gcgtagtggc atccgtaagt
    19921 ccggatgatc tgctgaaaac attgccgaag cgaaaagggt aaaacgccag ttttcgggtt
    19981 actcaccact catcaaatct gcatgatatt gcctgccggg taaggcgtca tgccgcatcc
    20041 ggcaacacac gactcaacgc tgtcccgctt ctggtttgcg cgatttttgc cagtaaaaat
    20101 gctcgcgcaa accttccgcc gactcttccg ccacagcacg caattcatcg ctgtccgctt
    20161 catgacgcag ctgatgatcc acattcttta cccacataaa ttcatgtcct ttgtgccctg
    20221 ccatgagttg tcctgagaac agtgcacacg ttaataagac aaccgataac gccttcgaaa
    20281 acatcccgcc acctttttct aaccttttgc cgctatgatg ccgatcgttt cttgaggtta
    20341 ttattcagtt ttgcaaatta gagcaaagaa attctggaat cttccttcct gattttgcat
    20401 tgcattctgc cgttgcggcg atttagtgct attttcgagc acattacaca cggaggtaaa
    20461 cttcgccatt aaatagtcac gatcacttta ttgaagtgcc tttgagcgtc gcctctaagg
    20521 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
    20581 ttggcaaaca taaacaggag attactcgcc tatttaattt gcaccatgcg acaaaaatcg
    20641 acgccgtcca gctcgcggct aatgcgctag gcaaagagtt atcgctggtg atggtttgat
    20701 aacagttaat gaaaagttgt catttttaac aactgatata gactgccgaa tcacctgcac
    20761 ataataacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
    20821 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
    20881 tttctggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
    20941 atattatcgc gcgaccgcaa tcgggttatt acgttgcgcc acaggcaata aaaatgccga
    21001 aagcgacagt cattccagtc acacgagatg aagccgtcga tatcaacacc tatatttttg
    21061 atatgttgca ggccagtcgt gatccgtcgg tagttccgtt tgcctcggcc tttcccgacc
    21121 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
    21181 cggcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
    21241 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attaccgccg
    21301 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
    21361 tagtagagaa tccttgtttc tacggcgcgt tgcaggcgct ggagcggtta cggctgaagg
    21421 cgttatcggt agcgacggat attaaagaag ggatcgatct tcaggcgctg gaactggcgt
    21481 tgcaggagta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccacttggat
    21541 ttaccttaac gccgcagaaa aaagtgcggc tggtagcgtt gctcaatcag tacaacgtaa
    21601 cgctgattga agatgacgtt tatagcgaac tttattttgg acgggaaaaa ccgctgcctg
    21661 cgaaagcgtg ggatcgccac gatggtgttt tgcattgctc ttcgttttcg aaatgtctgg
    21721 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
    21781 tgcagttgat gagtacgctt tccaccagct caccgatgca gcttgcgctg gtggattacc
    21841 tttctactcg ccgatacgat gctcatcttc gtcgcctgcg tcgccagctt gcggaacgta
    21901 aacaacgcgc ctggcaggct ttgctgcgtt atctgcctgc ggaagtcaaa attcatcaca
    21961 atgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgca ggagaattaa
    22021 gcctggtggc actgacgcat catatcagta ttgcgccggg taatatgttt tctaccggtg
    22081 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
    22141 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
    22201 tcctctcagg ttgggtaata tgaatttcga atagcagtca tatattctaa ctccttgtct
    22261 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
    22321 ttttctcagg gccgaaggtg tgcctgcaag ctgccgtcta tggttaaaca aggagatatt
    22381 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
    22441 tttgcccgca gcagcctgtg tgcgctcagc atgacaataa tgaccgctca cgccgccgaa
    22501 ccgcctacca atttagagaa tccggaaggg cgactggata ttatcgcctg gccgggatac
    22561 atcgaacgcg gacaaactga taaacagtac gactgggtaa cgcaattcga aaaagagaca
    22621 ggctgcgcgg tgaatgttaa aaccgccgcg acttccgatg aaatggtcag tctgatggcc
    22681 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
    22741 aaacgcgtgc agccaattaa taccgccttg attcccaact ggaaagcgct cgatccgcgc
    22801 gtggttaaag gcgactggtt taacgttggt ggcaaagttt acggcacacc ttaccaatgg
    22861 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
    22921 gtggtttttg ttgagcagaa tctgccggac ggcaagagca ataaaggccg ggtacaggct
    22981 tatgatggcc ctatctacat tgcggatgcc gcgttgttcg tcaaagccac gcagccacag
    23041 ttgggcatca gtgatccgta tcaactcacc gaagaacagt atcaggcggt gctgaaagtg
    23101 ctgcgcgatc aacataattt gatccatcgc tactggcatg acactaccgt gcaaatgagc
    23161 gatttcaaaa acgagggtgt ggttgcttcc agtgcatggc cctatcaggc caacgccctg
    23221 aaagccgaag gccagcctgt cgctaccgtt ttcccgaagg agggcgttac cggttgggct
    23281 gacaccacca tgctacatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
    23341 tggtcattaa ccccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccagta
    23401 gtgccgcaag ggtgtaaagc cagtccgtta ttaggcgaga aaggttgtga aacaaacggt
    23461 tttaactatt tcgataaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
    23521 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
    23581 tggggtgttt tatgacgtac gcagtggagt ttgacaacgt ctcacggttg tacggtgacg
    23641 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
    23701 ggccgtccgg ctccggcaaa accacttgcc tgcgcctgat tgctggattc gaacagcttt
    23761 ccggtggggc tatctctatt tttggtacgc ccgccagcaa tctgccaccg tgggagcggg
    23821 acgtgaatac tgtcttccag gactacgcgc tatttccgca tatgtcgatt cttgacaacg
    23881 tagcctacgg gctgatggtc aaaggcgtga ataaaaagca gcggcatgca atggcgcaag
    23941 aggcgcttga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
    24001 gtggtcagcg ccagcgagtc gccatcgcca gagcactggt gaacgaaccg cgtgtgttgt
    24061 tgctggatga accgctcggc gcgctggatc tcaaattgcg agagcaaatg cagcttgagc
    24121 tgaaaaagct gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
    24181 aagcgttatc gatgtccgat cgggtggcgg ttttcaataa tggacgcatt gaacaggtcg
    24241 attctccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgcggga ttcgttggga
    24301 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
    24361 cactacgacc ggaacatatc cgcctcaaca cccctggcga aatgcaggct aatggcacga
    24421 tccaggcggt gcaatatcag ggcgcagcga cccgttttga actgacactg agcggcggtg
    24481 aaaaactgct ggtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
    24541 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtggcgctg gttgaggaga
    24601 ggtgaatggc gatgaacgta ttgcaatcac cttcacgtcc aggtctgggt aaggtgtccg
    24661 gtttcttctg gcgtaatccg gggctggggc tgtttttgct gctgcttggc ccgctaatgt
    24721 ggtttggcat tgtctatttc ggctcgctgc tgacgctgtt atggcagggc ttttatacct
    24781 ttgacgattt caccatgtcg gtaacgccgg aactgacgct ggcgaatatc cgtgcgctgt
    24841 ttaatccggc gaattacgac atcattgtcc gcacgctgac catggctgtg gcagtcacta
    24901 tcgccagcgc cattctggct tttccaatgg cgtggtatat ggcgcgctat accagcggaa
    24961 aaatgaaagc gtttttttat attgcggtaa tgttgccgat gtgggcgagc tacattgtta
    25021 aagcctatgc ctggacgtta ttgctggcaa aagatggcgt ggctcagtgg tttttacaac
    25081 atcttgggct ggaaccactg ctgactgcgt tcctcacgct accggcggtg ggaggtaata
    25141 cgctgtcaac ttccgggctg gggcgctttc tggtgtttct ctatatctgg ctgccgttca
    25201 tgatcctgcc cgtgcaggcg gcgcttgagc gtttgccgcc gtcattgttg caggcgtcgg
    25261 ctgatcttgg cgcacgtcca cgacaaacct ttcgctatgt ggtgttgccg ctggcaatcc
    25321 cgggtattgc cgctggctct atctttacct tctcactcac gctgggcgat tttattgtcc
    25381 cgcagctggt tggccctcca ggatatttta tcggcaatat ggtttattcc cagcaggggg
    25441 cgattggcaa tatgccgatg gcggcggcat tcaccctggt gccgattgtt ctcatcgcac
    25501 tttacctggc gttcgtgaaa cgtctgggag cgttcgatgc actctgaacg cgcaccgttt
    25561 ttcctcaaac tggcggcctg gggcggcgtg gttttcctac attttcccat cctgattatc
    25621 gctgcctatg cgtttaacac tgaagatgcg gcgtttagtt ttccaccgca aggcctgacg
    25681 ctgcgctggt ttagcgtggc agcacagcgt agtgatattc ttgatgccgt gacgctgtca
    25741 cttaaagtgg cggcgctggc gacattaatc gcgctggtgt tagggacgct ggccgctgcc
    25801 gcgctgtggc gacgagactt tttcggcaaa aacgccattt cgctgttact gttgctgccc
    25861 attgcgctgc cgggcattgt cactggtctg gcgctattaa ccgcctttaa aaccatcaat
    25921 ctggagccgg gatttttcac catcgtggtc ggtcatgcga ctttttgcgt agtggtggtg
    25981 tttaacaatg tcatcgcccg ttttcgccgc acctcgtgga gtctggttga ggcgtcaatg
    26041 gatcttgggg ccaatggctg gcaaaccttc cgctacgtag tgttgccgaa tctcagttcg
    26101 gcgttactgg ctggaggaat gctggcgttt gccttgtcgt tcgatgaaat catcgtgacg
    26161 acttttacgg caggtcatga acgaacgtta ccgttgtggt tgctcaatca gcttgggcga
    26221 ccgcgtgatg taccggtaac caacgtggtg gcactgctgg ttatgttggt aacaaccttg
    26281 ccgatcctgg gggcctggtg gctaacccgc gaaggcgaca atggtcaata accactgata
    26341 caggaatatg ctatgcaaca taagttactg attaacggag aactggttag cggcgaaggg
    26401 gaaaaacagc ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggca
    26461 tccgcagagc aggtcaatgc tgctgttcgc gcggcagatg cagcatttgc cgaatggggg
    26521 caaaccacgc caaaagcgcg tgcggaatgc ctgctgaaac tggctgatgt tatcgaagaa
    26581 aatggtcagg tttttgccga actggagtcc cgtaattgtg gcaaaccgct gcatagtgcg
    26641 ttcaatgatg aaattccggc gattgtcgat gtctttcgct ttttcgccgg tgcggcgcgc
    26701 tgcccgaatg gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc
    26761 gatccgttgg gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggctgcg
    26821 tggaaactgg ctccggcgct ggcggcaggg aactgcgtag tgctaaaacc atcagaaatt
    26881 accccgctga ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggctggcgtg
    26941 attaatgtgc tgtttggcag aggcaaaacg gtgggagatc cgctgaccgg acatcccaaa
    27001 gtgcggatgg tgtcgctgac gggttctatc gccactgggg aacacatcat cagccatacc
    27061 gcgccgtcca ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt
    27121 gatgatgcgg atattgaagt agtggtcgaa ggtgtacgta cttttggcta ttacaatgcc
    27181 ggacaggatt gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg
    27241 gtggaaaaac tcggtgctgc ggtggcaacg ttaaaatcgg gttcgccgga tgacgagtct
    27301 acggagcttg gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcggtagaa
    27361 gaggcgaaag cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat
    27421 ggctattact atgcgccgac gctgctggct ggagcattac aggacgatgc catcgtgcaa
    27481 aaagaggtat ttggtccggt agtgagtgtt acgctcttcg acaacgaaga acaggtggtg
    27541 aactgggcga atgacagtca gtacggactt gcatcttcgg tatggacgaa ggatgtgggc
    27601 agggcgcatc gcgtcagcgc gcggctgcaa tatggttgta cctgggtcaa tacccatttc
    27661 atgctggtaa gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg
    27721 tcactttatg ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagga
    27781 taatattgca gatcgtaaga gtatgagatg atcttgcgta ctgtcagagc actgatttct
    27841 ctgacagtac gtgctgcgtg caaaatcaca acatcaaata aaccagccaa atctcataga
    27901 gataagagta aacaaaccaa cagcagcaaa gacatttatc agtactacag ttctactgga
    27961 aacattcatt tttgccacct tttatcattg cccctatgtg tatagtacgg gttaagaaaa
    28021 tccgcaataa tactcattct gaagatagat aagggcaagt tgctgtttga tgattttttc
    28081 tctaactggt gaaaaaactt gatgcacgtc aaaaaatgac gcatatttgc gcgcgtttta
    28141 ttcatctggc tggacgctcg tacatgtctc tctatcaaca tatgcttgtt ttttatgcgg
    28201 ttatggcagc aatcgcattt cttatcacct ggtttctttc tcacgataag aaacgcatcc
    28261 gtttcttaag cgcctttctg gtgggggcaa catggccgat gagtttcccg gtggcgctgt
    28321 tgttttctct gttttaaatc ttatgcactg gcttgacggc accacgcaga cttatatcat
    28381 ttggatgaat cgataaattt cacaagtggc taaggagaaa gtatgtcgca tctggatgaa
    28441 gtcatcgcgc gcgtggatgc cgcgattgaa gagagcgtca ttgcccatat gaacgaatta
    28501 ttaatcgccc tgagcgatga cgcggagtta agtcgggaag atcgctacac ccagcagcag
    28561 cgtttgcgca ccgctattgc tcatcacggt cgcaaacata aagaagatat ggaagcgcgc
    28621 cacgaacagt taaccaaagg cggtaccatc ctctgattaa aatgagcgtc tggccaccag
    28681 ccaggcgcca accacaagta aaattgcgcc acaaattggg ccgatcaacg cccgaagtgg
    28741 tacgccatgg ctgcggaaga tatccagcac cagaccgcca atcagctggc tggcgaccag
    28801 tactgcaata gttgtcgccg ctcccacatt ctgatatccg ctgatactgg cgaagacaaa
    28861 aaacgatccc agtaagccag gtataagtgt ccaccagcgc acgctggaca ccagttcgcc
    28921 aaaccccgcc atgccttgtt taaaccataa aatagaaaca aagagcacaa tccccaccag
    28981 tgagttcagc aacatggcga tgagaatggt agaggaggtc tgggtgatgc gcaccatcag
    29041 cgtgttctgg actaccagac caatcccggc tgcaatcaaa aaggcaaggg taagtgactg
    29101 actcatccaa tcgcgtccgg ttcagtgcgc tcgtcgagtt gcaactgcat aaatgtcaga
    29161 tccagccagc gcccaaattt agtgcctacc tgcggcattt gcgcggtgac gacaaatccc
    29221 agcgactggt gcagatgcag cgaggcctga ttttgcgatt cgatcccggc gaccatgaca
    29281 tgcttcccgc aatcccgcgc ttcatcaatc aatcggctta acaatttacg ccccagacct
    29341 ttcccctgat gatcgggatg gacataaacc gaatgttcca cggtatggcg aaaaccatca
    29401 aaactacgcc agtcgccaaa cgaggcatat cccgtcacta cgccatcttc ttcactcacc
    29461 agcaccggat aacctgctat ggtccgcgct tcaaaccagg caatgcggtt atccgcatcc
    29521 accgtctggt cattccagat agccgccgta tacaacacgg cgtggttata aatttccgca
    29581 attgcagcac agtcggcttt gcgggcaaaa cggatggaca tgtctggcct cgattaaata
    29641 atgatgatga tgttatcaat actatcgaaa acagcccgaa gcaacggatt cctctatgaa
    29701 attattgcgt gttgcagagg ggatgtgacg gctgcaaaca aaaccaatcc tgtgagcctg
    29761 ctactattga tggattcatg ccaaaagcac aggagagcat tatggggcaa caaacgcagc
    29821 gtaaccgatg ttgggttctg gcctcgcgtc cgcatggcgc gccggttcct tacgtctggt
    29881 ttcgataaat gcgctctttg tcgcaccagc gcaagcaatt acagttccgg aagtgggaaa
    29941 acgtcaatta aatgaaatca atcggatccg ctacgaactg gaattaatgg cagttgctct
    30001 ggctgtcgaa aacctcacta tgcaaaaaaa cgttgctatg ttgtgccagc aatacagccg
    30061 ataaataaaa tacgcccggc aaataccggg cttaaagata attactcaaa tcgccacgca
    30121 atatttatgc cgacaccata attttgtccg ggcgcaggtt cgtaatatcg cccgtttgac
    30181 tcattgacaa tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat
    30241 aaatccacgg ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagtgta
    30301 taagaaggcg ctttggccgt attttcatca tctgccataa tatcgcccat ataacgcgct
    30361 tccgtgcctg cataccaacc atcttccggt atataaccta tcgacgcaaa gcccatatta
    30421 cgggcgatcc ctggcatccg gttaccgtta caatcctgtt cattgcaaac attgctgcga
    30481 taggtcgcat caagccaggt ccaggacgct tttacgcgaa aatctcccgc gaaacgttga
    30541 tcccatgcca gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc
    30601 ccaccgctac tgctatcgac aacaatttca tcatcggtgt cggtctggaa caatgcgaga
    30661 ctaagcagcc catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggag
    30721 ggttttaaac caaagttcat accgctttgc ccatcagcgc gataagacag ctcattaatc
    30781 gtcggcgttt caaaatcgcg cccggctgcc agatagacat tccaggcatc ggtcattgca
    30841 tattttaacg atccggctgg tagccatttg tgataactgg catcaccgct gtcgtcgctg
    30901 ttacccggag taacgtaatg atcattggaa tcaaaccaca cggagctgta gcgcacgcca
    30961 gcatccagcg aaagtttgtc ggtcagttgc cactgggttt gtaaataggg atcgacgttc
    31021 cacatcaaat tgcgttcgtc gcggcgcaac tcaccttttt gcccgtattc cggcacgccg
    31081 cgattcaggc ggaagttatt gtaacccttg cggttttcac tcatgttttc gtagttcagg
    31141 ccggttgtga acgtgactgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc
    31201 ccctgataat ggcgttgcag agtaatcacg ccgcccgcat gtgacgggtt aagttgggga
    31261 gccatcggga ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc
    31321 atatcatctt gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg
    31381 gtttttcgcg tgtcgtactg ttctgcacga ggcgcttgtt gtggattcgc cttccattct
    31441 gctttggtta gcccacctgg gtcatctgct ttgatatcca cactattgaa aatcaggctt
    31501 aatttgctgg cgtcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg
    31561 ccgctatggt cacgatagcc gtgggtcgta aaacgtgtgg ttgagacggt gtaatcgaca
    31621 tcgccaggct gtgtgccgtc gcccgttgcg cctgttgctt tcagcccata gcgccagctg
    31681 ccaaaactgc cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg
    31741 atgacattca ttacaccgcc agacgcgttg ccatacagag cagagaaggg gccacgcagc
    31801 acttccacat tttgcacact gcttaaatcg atgttggatg tctgcccttg tccgtcgggc
    31861 atggtggcgg gaataccgtc cacatacagg cgaataccac gaattccgta agtggagcgg
    31921 gagccaaatc cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc
    31981 tgtaaaccag gcacgccggt cagtgattca gacaagttaa tgcgcggtgt tgccaggcgc
    32041 atctcctcgc catccaccac gctcacggct gctggggtat ccagttctga aaccacctgc
    32101 ggtgcggcac tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaaggaca
    32161 agcagtgcgg gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta
    32221 agaatgggaa aacaccgtca tggtaatgaa attgtaaatt tatggaaaac gaaacggcac
    32281 aatacgttaa gtaattgaga aaattgtagt cgtaacggta agaaatgctc cacatttgag
    32341 aaaataatga ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa
    32401 gcattgtgaa gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat
    32461 ctgttttcat cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca
    32521 ttcagtacgc aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa
    32581 atggcttata gccagcaaga aaacgcgctg tggcttgcca cttcgcaaag ccgcaaactg
    32641 gataaaggcg gcgtggttta tcgtcttgat ccggttactc tggaagtgac gcaggcgatc
    32701 cataacgatc tcaagccgtt tggtgccacc atcaataaca cgactcagac gttgtggttt
    32761 ggtaacaccg taaatagtgc ggtcacggcg atagatgcca aaacgggcga agtgaaaggt
    32821 cgtctggtgc tggatgatcg taagcgcacg gaagaggtac gcccgttgca accacgtgag
    32881 ctggtagctg atgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg
    32941 atttgggtcg ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa
    33001 atgagtaccg gtctggcgct ggatagcaaa ggcaaacgtc tttacaccac taacgctgac
    33061 ggcgaattga ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg
    33121 gatgacggca aagagcactt ctttatcaac atcagccttg ataccaacaa tcagcgtgca
    33181 tttatcaccg attctaaagc ggcagaagtg ttagtggtcg atacccgtaa tggcaatatt
    33241 ctggcgaagg ttgcggcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa
    33301 gcctacgtga cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa
    33361 gtggtgaaaa cgttcgatac gccgactcat ccgaacagcc tggcgctgtc tgccgatggc
    33421 aaaacgctgt atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagccg
    33481 gacgatgtga ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gaaagaattg
    33541 cctgactttt atcacgccgc atccggcacc ttcgtcagta gtctgaattt gcccggcacg
    33601 tagccgggca attttgcaat taactcgacg tttcaggctt aaacacaatt tcctgttttt
    33661 cttcatcttc ctcgacgact ggcgcagtgc tgtgaatttc agcaacgcgt ttgcgcacac
    33721 caaaccagcc tataaccagc agaataccga taatcggcag tgccgcgatg gtataagtcc
    33781 cgttcgggta atcgaacgcc atcagcacca ggacactcag taaaaacagt aatgtcagcc
    33841 aggaagtgaa gggcgcgcca ggcagtttaa aactgacatc cgctgctttg ccttctttaa
    33901 tcgctttacg caggcgcatc tggcaaacga tgataaacgc ccatgaagcg atgattccca
    33961 gcgacgcgaa gttcaacaca atctcaaata cgcgtgacgg caccagatag ttgaggaata
    34021 cgccgacgac atacacaacc agtgtcgcca gaatcccggc atacggcaca tgctgacgac
    34081 tcattttcgc cataaaactc ggtgcggaac cgcccatcgc catcgagcgc agaatacgcc
    34141 cggtgcagta cagacctgaa ttcaggctgg agagggcagc ggtcagcacc acaatgttca
    34201 taatgctgcc gatgtacggc acacccagtt tagagaaaaa cgtcacgaac ggactttgcc
    34261 ccgcctgata cgcgctccac ggcaataaca taaccagcaa caccacggag ccgacgtaaa
    34321 acagaccaat acgccaaatc acactgttaa tcgctttagg caccatggtc tggggatctt
    34381 tacattctcc ggccgctgta cccaccattt caatggaggc aaaagcaaac actacgccct
    34441 gaatcaacac cagcgcaggc agcaaaccgt gggggaagaa gccgccatta tcggtgatta
    34501 aatgaaagcc agtggtgttg ccatccagcg gctgaccact accgaggaac actgtaccca
    34561 cgaccaaaaa ggttacaatg gcgagcactt taataagcgc aaaccagaac tccatctccg
    34621 caaaccattt cacaccgatc atattcatgg tgccaacgat ggtcagtgca gcgagcgcaa
    34681 agacccactg cggcacgccg ccaaacgcgc cccagtaatg catatacaga gcgacggcgg
    34741 taatatcaac aatccccgtc atcgcccagt tgatgaagta catccagcca gcaacataag
    34801 cggccttctc acccaaaaac tcacgggcat aagagacaaa actgccactg gaagggcggt
    34861 gtaacaccag ctcacccaat gcacgcagaa taaaaaacga aaacaggccg cagatcaaat
    34921 agaccagtgc cagtgcgggc cccgccattt gcagtcgcgc ccccgcgcct aaaaacaaac
    34981 ccgtaccaat cgcgccgcca atggcgatca tctgtacctg gcgattgccc atcgctttgt
    35041 gatacccctc ttcgtgggca ttaagccagc gacgtttcgc ggcgtgttga tctgaagtgt
    35101 cggtgttgtg tttactcatt gctctccctg attgctttaa tgaaaaagtc atataagttg
    35161 ccatgaacaa tgtttattca tcctggcgat gatagttatc accattattc tttatgggta
    35221 taaaactatc ggcgcagcat cctacccgtt atcgataaac gatgcaaaac atccccttac
    35281 aatcctgaag gggatttata caactgacga aaaaatgata aatccttttg ctggataacc
    35341 tgtgtgctgt cctacactta atctttaaaa gattgtgagg ggcatatgat taaggtgtat
    35401 ggcgtacccg gctggggctc tacaatcagt gagctgatgc taacactggc tgatattcct
    35461 tatcagttcg ttgatgtcag tggttttgac cacgagggaa cttcacgcga tttattaaaa
    35521 accctgaatc cgttgtgtca ggtcccgaca ctggcgctgt aaaaataacg agattatgac
    35581 ggagacagcg gcgattgcat tgatggttct cgatcgtcgc ccggatcttg cgccacctct
    35641 tgggcgcgcc gaacgtcaac agtttcaacg gctattggtc tggctggttg ctaatgtcta
    35701 tccaacgttc actttcgccg attaccctaa gcgttgggca tctgacgcac ctgtaattga
    35761 atatcgcaaa tcgctttata tctggctgaa ttcgcaattg actgctgaac cgtatgtgtt
    35821 tggagtgcaa ctaacgctgg tggactgtta tctctgcacc atgcgcacat ggggaccggg
    35881 acacgaatgg tttcaggata atgccccgaa cattaatgcg attgctgatg ctgtatgtca
    35941 gataccgaaa ttacaagaag tattaaaaag gaatgtcatt atttaacaat aatgccggag
    36001 gttaatgtta atgaatcact ggcgtagtta actggacgtc catttgtttg ctatgaatag
    36061 caattaatgt tggcacaact attcatatgg ttgatgtgat ttcgtttttt atattaaata
    36121 tcaatttaat ataatctgaa aataaaacat cttttcgcat gtagagaaac atgtcgatat
    36181 gatagcgcag tgcagatcat gttatcaggg ctgggcggta gcgtatttaa tcagggaaag
    36241 attacgtaac actgcttttt tgtactggac gattctcatt ttatgtttaa aaattgagat
    36301 actccttatt tcctgaa
//
LOCUS       DAZFRE010000046        35651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFRE010000046 DAZFRE010000000
VERSION     DAZFRE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.046"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            83..1222
                     /gene="arnB"
                     /locus_tag="ACHKUI_002591"
     CDS             83..1222
                     /gene="arnB"
                     /locus_tag="ACHKUI_002591"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6598768.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1226..2194
                     /gene="arnC"
                     /locus_tag="ACHKUI_002592"
     CDS             1226..2194
                     /gene="arnC"
                     /locus_tag="ACHKUI_002592"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6598769.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2194..4176
                     /gene="arnA"
                     /locus_tag="ACHKUI_002593"
     CDS             2194..4176
                     /gene="arnA"
                     /locus_tag="ACHKUI_002593"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6598770.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4173..5063
                     /gene="arnD"
                     /locus_tag="ACHKUI_002594"
     CDS             4173..5063
                     /gene="arnD"
                     /locus_tag="ACHKUI_002594"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6598771.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTVQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSH"
     gene            5063..6715
                     /gene="arnT"
                     /locus_tag="ACHKUI_002595"
     CDS             5063..6715
                     /gene="arnT"
                     /locus_tag="ACHKUI_002595"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6598772.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDEMRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRNKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6712..7047
                     /gene="arnE"
                     /locus_tag="ACHKUI_002596"
     CDS             6712..7047
                     /gene="arnE"
                     /locus_tag="ACHKUI_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6598773.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7047..7433
                     /gene="arnF"
                     /locus_tag="ACHKUI_002597"
     CDS             7047..7433
                     /gene="arnF"
                     /locus_tag="ACHKUI_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6598774.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7427..7693)
                     /gene="pmrD"
                     /locus_tag="ACHKUI_002598"
     CDS             complement(7427..7693)
                     /gene="pmrD"
                     /locus_tag="ACHKUI_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6598775.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7803..9158)
                     /gene="menE"
                     /locus_tag="ACHKUI_002599"
     CDS             complement(7803..9158)
                     /gene="menE"
                     /locus_tag="ACHKUI_002599"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6598776.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSSVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVKRQ
                     Q"
     gene            complement(9155..10117)
                     /gene="menC"
                     /locus_tag="ACHKUI_002600"
     CDS             complement(9155..10117)
                     /gene="menC"
                     /locus_tag="ACHKUI_002600"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6598777.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCEIPQMPSVAFGVSCALAELAETLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10117..10974)
                     /gene="menB"
                     /locus_tag="ACHKUI_002601"
     CDS             complement(10117..10974)
                     /gene="menB"
                     /locus_tag="ACHKUI_002601"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6598778.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10989..11747)
                     /gene="menH"
                     /locus_tag="ACHKUI_002602"
     CDS             complement(10989..11747)
                     /gene="menH"
                     /locus_tag="ACHKUI_002602"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6598779.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQEPAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11744..13414)
                     /gene="menD"
                     /locus_tag="ACHKUI_002603"
     CDS             complement(11744..13414)
                     /gene="menD"
                     /locus_tag="ACHKUI_002603"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6598780.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13503..14798)
                     /gene="menF"
                     /locus_tag="ACHKUI_002604"
     CDS             complement(13503..14798)
                     /gene="menF"
                     /locus_tag="ACHKUI_002604"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6598781.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14877..15182)
                     /gene="elaB"
                     /locus_tag="ACHKUI_002605"
     CDS             complement(14877..15182)
                     /gene="elaB"
                     /locus_tag="ACHKUI_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6598782.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEMLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15237..15698)
                     /gene="elaA"
                     /locus_tag="ACHKUI_002606"
     CDS             complement(15237..15698)
                     /gene="elaA"
                     /locus_tag="ACHKUI_002606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6598783.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15763..16680
                     /gene="rbn"
                     /locus_tag="ACHKUI_002607"
     CDS             15763..16680
                     /gene="rbn"
                     /locus_tag="ACHKUI_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6598784.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFNV"
     gene            16868..16984
                     /locus_tag="ACHKUI_002608"
                     /pseudo
     CDS             16868..16984
                     /locus_tag="ACHKUI_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            17036..18007
                     /gene="yfbL"
                     /locus_tag="ACHKUI_002609"
     CDS             17036..18007
                     /gene="yfbL"
                     /locus_tag="ACHKUI_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO6598785.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGVDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            18110..18613
                     /gene="yfbM"
                     /locus_tag="ACHKUI_002610"
     CDS             18110..18613
                     /gene="yfbM"
                     /locus_tag="ACHKUI_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6598786.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(18680..20137)
                     /gene="nuoN"
                     /locus_tag="ACHKUI_002611"
     CDS             complement(18680..20137)
                     /gene="nuoN"
                     /locus_tag="ACHKUI_002611"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6598787.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SASGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(20144..21673)
                     /gene="nuoM"
                     /locus_tag="ACHKUI_002612"
     CDS             complement(20144..21673)
                     /gene="nuoM"
                     /locus_tag="ACHKUI_002612"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6598788.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(21837..23678)
                     /gene="nuoL"
                     /locus_tag="ACHKUI_002613"
     CDS             complement(21837..23678)
                     /gene="nuoL"
                     /locus_tag="ACHKUI_002613"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6598789.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(23675..23977)
                     /gene="nuoK"
                     /locus_tag="ACHKUI_002614"
     CDS             complement(23675..23977)
                     /gene="nuoK"
                     /locus_tag="ACHKUI_002614"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6598790.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(23974..24528)
                     /gene="nuoJ"
                     /locus_tag="ACHKUI_002615"
     CDS             complement(23974..24528)
                     /gene="nuoJ"
                     /locus_tag="ACHKUI_002615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6598791.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(24540..25082)
                     /gene="nuoI"
                     /locus_tag="ACHKUI_002616"
     CDS             complement(24540..25082)
                     /gene="nuoI"
                     /locus_tag="ACHKUI_002616"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6598792.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(25097..26074)
                     /gene="nuoH"
                     /locus_tag="ACHKUI_002617"
     CDS             complement(25097..26074)
                     /gene="nuoH"
                     /locus_tag="ACHKUI_002617"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6598793.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(26071..28797)
                     /gene="nuoG"
                     /locus_tag="ACHKUI_002618"
     CDS             complement(26071..28797)
                     /gene="nuoG"
                     /locus_tag="ACHKUI_002618"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6598794.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIIGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(28850..30187)
                     /gene="nuoF"
                     /locus_tag="ACHKUI_002619"
     CDS             complement(28850..30187)
                     /gene="nuoF"
                     /locus_tag="ACHKUI_002619"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6598795.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(30184..30684)
                     /gene="nuoE"
                     /locus_tag="ACHKUI_002620"
     CDS             complement(30184..30684)
                     /gene="nuoE"
                     /locus_tag="ACHKUI_002620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6598796.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(30687..32489)
                     /gene="nuoC"
                     /locus_tag="ACHKUI_002621"
     CDS             complement(30687..32489)
                     /gene="nuoC"
                     /locus_tag="ACHKUI_002621"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6598797.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(32583..33245)
                     /gene="nuoB"
                     /locus_tag="ACHKUI_002622"
     CDS             complement(32583..33245)
                     /gene="nuoB"
                     /locus_tag="ACHKUI_002622"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6598798.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(33261..33704)
                     /gene="nuoA"
                     /locus_tag="ACHKUI_002623"
     CDS             complement(33261..33704)
                     /gene="nuoA"
                     /locus_tag="ACHKUI_002623"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6598799.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(34333..35271)
                     /gene="lrhA"
                     /locus_tag="ACHKUI_002624"
     CDS             complement(34333..35271)
                     /gene="lrhA"
                     /locus_tag="ACHKUI_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6598800.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDIVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         8607 a         9748 c         9141 g         8155 t
ORIGIN      
        1 tttgctccaa atggcaaaat atacacaaca ctctttatag caaatataag tggacaggta
       61 ttcaatgacg gatggaaaaa caatgtcagg atttttgcct ttttcgcgac cagcaatggg
      121 cgtagaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca caaccggtcc
      181 gaagaatcag gcgcttgaac aagctttttg ccagttgacg ggaaatcagc atgccatcgc
      241 ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa ttggcaaggg
      301 cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga tttccttgct
      361 gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca cgcctgaagc
      421 gatcgaagca gccattacgc cacgcactaa agccatcatt ccggtgcatt atgccggtgc
      481 gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg cagttatcga
      541 agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg caaaaggtac
      601 tgctattttt tcgtttcacg ccatcaaaaa tattacctgt gcggaaggtg gcctgattgt
      661 tactgataat gaaaatcttg cccgccagct gcggatgctg aaatttcacg gtctgggtgt
      721 cgatgcctat gacagacata cctggggccg tgcaccgcag gctgaagtct taacgccggg
      781 ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt tagtcaaatt
      841 agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag cactggcagc
      901 tctccccttt cagccattaa gccttcccgc ctggccgcac gttcatgcct ggcatctgtt
      961 tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga tggaagcgtt
     1021 aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac aaaaatatta
     1081 tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg aacgcatctg
     1141 ttcgttgccg ctgttcccgg atatgaccac cgccgatgcc gaccgcgtca tcacagccct
     1201 tcagcaactc gcaggacaat gagccatgtt tgaaatccac cctgttaaga aagtctcggt
     1261 ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc gcaccaccgc
     1321 agcctgtgaa tcactgggga aagagtatga aatcctgctg attgatgacg gcagtagcga
     1381 taattccgcg catatgctgg tcgaagcctc acaagcggag ggcagccata ttgtgtctat
     1441 cttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca gtcacgtcac
     1501 cggcgactta attattaccc tcgatgccga tctccagaat ccgccagagg aaatcccccg
     1561 tctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc agaaccgcca
     1621 ggacagctgg tttcgtaaaa ccgcttcgaa gatgattaac cggcttattc agcgcaccac
     1681 tggcaaagcg atgggtgact atggttgtat gctgcgcgcc tatcgccgtc atattgtcga
     1741 tgcgatgttg cactgccatg aacgcagcac ctttatcccg attctggcga atatcttcgc
     1801 ccgccgtgcc attgaaattc cagtgcacca tgccgagcgt gagtttggtg aatccaaata
     1861 cagctttatg cgcctgatta atttgatgta cgacctggtg acctgcctta ccacaacgcc
     1921 gctacgtatg ttgagtttgc tcggcagcat tattgcgatt ggcggtttta gcattgcggt
     1981 gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag gcgtctttat
     2041 gctatttgcc gtactgttta cttttattgg cgctcagttt atcggcatgg gattgctcgg
     2101 tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt ttgttcagca
     2161 agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt tgcctaccac
     2221 gatatgggat gcctcggtat tgaagccctg ctggctgccg gttacgaaat tagcgctatt
     2281 tttactcata ctgataatcc cggtgaaaaa gccttttatg gttcggtagc tcgtctggcg
     2341 gcggaaagag gcattccggt ttatgcgccg gataacgtta atcatccgct gtgggtggaa
     2401 cgcattgccc aactatcacc agaggtgatt ttttcttttt attatcgcca tcttatttgc
     2461 gacgaaattt tgcagctcgc tcccgcaggt gcatttaatc tgcatggttc gctgttaccc
     2521 aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac ggaaactggc
     2581 gttacattgc accgaatggt gaaacgtgcc gatgccgggg ccattgtggc acaactgcgc
     2641 gttgccattg cgccagacga tatcgccatt acgctgcatc ataaattgtg ccatgccgcg
     2701 cgccagctac tggaacagac attacccgcc attaaacacg gaaatattct ggaaatcgcc
     2761 cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc cggatgacag tttcctcgaa
     2821 tggcataagc cggcatccgt actgcacaac atggtgcgtg ccgttgccga tccgtggccg
     2881 ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg tgttcatcct
     2941 catgccagca aagcacagcc ggggagcgtg atttctgttg caccactgct gattgcctgt
     3001 ggcgatggcg cgctggaaat cgtcactgga caggcgggcg acggcattac tatgcagggc
     3061 tcgcaattag cgcagacgct gggcctggtg caaggttcac gcttgaatag ccagcctgcc
     3121 tgtgccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt tattggcaac
     3181 catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct ggatattggc
     3241 agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga aggcgatatc
     3301 agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt gttgccgctg
     3361 gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt tgaactcgat
     3421 ttcgaagaga atctgcgcat tatccgctac tgcgtgaagt accgtaagcg aatcatcttc
     3481 ccgtcgactt cagaagttta tgggatgtgt agcgataaat acttcgatga ggatcattct
     3541 aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc taaacaatta
     3601 cttgatcggg tgatctgggc ctatggcgaa aaagaaggtt tacagttcac cctcttccgc
     3661 ccgtttaact ggatggggcc acgactggat aaccttaatg cagcgcgaat tggcagctcc
     3721 cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccgattaa gctgattgat
     3781 ggcggaaaac aaaaacgctg ctttactgat attcgggatg gtatcgaggc gttataccgc
     3841 attatcgaaa atgcgggaaa tcgctgcgat ggcgaaatta tcaacattgg caatcctgag
     3901 aacgaagcga gcattgaaga actgggtgag atgctgctgg cgagcttcga aaaacatccg
     3961 ctgcgccatt acttcccacc gtttgcgggc tttcgtgttg tcgaaagtag cagctactat
     4021 ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc ccgccgctgc
     4081 ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct ggatttcttc
     4141 ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta cgcattgatg
     4201 tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc ttgagtaagc
     4261 ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc cgccatctct
     4321 ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg gcatcgcttt
     4381 atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt ggtcatgcca
     4441 atgctgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac gcctgggatc
     4501 accatgcctg gcaagcccat agcggtaatt gggatcggca aacaatgatc gacgatattg
     4561 cacgcggcct tcgcaccctg gaagagatta tcggtcaacc ggtaacctgt tctgccgctg
     4621 ctggctggcg tgctgaccaa caggtgatcg aagcaaaaga agcgttccat ttgcgctaca
     4681 acagcgattg tcgtggggcg atgccgtttc gtccattgct cgaatcagga acccccggca
     4741 ctgtgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg gatgtgaaag
     4801 cagaagattt taacggttgg ttactcaacc gcatccagcg agataaaggc acgccggttt
     4861 ataccattca tgccgaagtt gaaggctgcg cttatcagca taattttgtg gatctcctga
     4921 aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactatta tcagggacgt
     4981 tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg ctgggttgcc
     5041 aacaaattgc gggtagtcac tgatgaaatc ggtacgttac cttatcggcc tcttcgcatt
     5101 tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac cagatgaaat
     5161 gcgttatgcg gaaatcagtc gggaaatgct ggcatccggc gactggattg ttccccatct
     5221 gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca gcattgggca
     5281 atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg cgaccctgtt
     5341 aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcaataaac gtctggcttt
     5401 actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca cttatgccgt
     5461 gctcgatccg tttattgcct tctggctggt ggcgggaatg tgcagcttct ggctggcaat
     5521 gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa tcacctgcgg
     5581 catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa gcgtgctgcc
     5641 atgggtagca acgcaaaaac gctggaaaga tctctttatt tacggctggc tggcggttat
     5701 cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg agcctgactt
     5761 ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg atgcccaaca
     5821 tagagcgccg ttctggtact acttgccagt catcattgcc ggtagcctgc cgtggctggg
     5881 attactcccc ggtgcactgt acgctggctg gaaaaaccgc aagcattccg caaccgtcta
     5941 tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag gcaaattgcc
     6001 cacctatatt ctttcctgct ttgcacctct ggcaatgctg atggcgcatt acgcgttgct
     6061 ggcagcaaaa aataatcctc tggcgttgcg gattaatggc tggattaaca tcgcttttgg
     6121 cgtcactggc attattgcca catttgtggt ctccccctgg ggaccaatga acacaccggt
     6181 gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tctatttttt cgctatgggc
     6241 ttttttcggc tggtacacct taacaaacgt cgaaaagacc tggccttttg ccgcgctttg
     6301 cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca tggaaggaaa
     6361 acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc gctatattct
     6421 tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg atgacatcat
     6481 catgtatcgc cagacaggtg agttgaaata tggccttaat tatccggatg caaaagggag
     6541 atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg aggggattat
     6601 tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca ttccgcccgc
     6661 agatgtcatc gatcgtcagg agcgtctggt gctaattcag tatcggccca aatgatctgg
     6721 ttaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca gaaacaggca
     6781 acctgctttg cggcggtaaa caaacggcgc aaacatatcg tgctgtggct gggactggcg
     6841 ctggcttgtc ttggccttgc catggtgctc tggctgctgg tcttgcagaa cgtaccggta
     6901 ggcattgcct acccgatgtt aagtctgaat tttgtctggg taacgctggc tgcagtaaaa
     6961 ctgtggcacg agccggtatc gctgcgtcac tggtgtgggg tggcgttcat tattggcggc
     7021 attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc agcgtcatta
     7081 tcgcctccgc tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg ccaatgacgc
     7141 acttatggga ttttatcgcg gcgctgttag cttttggcct ggatgcccgg atactgttac
     7201 tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacacta cataaacttg
     7261 cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg attgcttcaa
     7321 tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga gtagcctgta
     7381 ttatgagcgg gttgatgctg attttcctgc ccacaacaaa acaacgttac tgagttttcc
     7441 ctgccgcttt acactgccgt tcccactcat cggggctata acagcttgca gaaaggactt
     7501 tcaccgtgtg cagcttttca cgattaatac agtaaagcgc attctgcaaa ggcgagagta
     7561 aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt atcgcgccgc
     7621 cagcatcgca gagcataatt acatgtctgt tatcttgttt attgcagcac gattttttga
     7681 ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt tacagattaa
     7741 cagataaact ctttatcaac ggcaatggaa tggcagaaat gagaagtgaa acggtgggtt
     7801 aattattgct ggcgtttcac ccactctttt agcgcctgac gtgaaatttt aataccgccg
     7861 tttttcagct ccggcggcag agtcagccag cgcaccggtt gttgaaaacg agccagctta
     7921 tctttcaccc attcgtcaag atcaacgctt tgctggtcat actccaccac cgctaccggc
     7981 cgatgaccaa actccttgtc ggcaacggga acgataaaca cctgcaaaac cgcaggatgt
     8041 gcagcaataa cgcgctcgac ttcttccggc tgaataccct caccgccact gaagaataga
     8101 ttgtctaaac gtccgacaat ggtcaacttg ccattatgca tctcaccgcg atcgcgcgtg
     8161 gcgtaccagc cttcgtcatt aaccagtgga acccgttgcc cgttacgcca gtaaccttct
     8221 gccatactgg cagcccgcag ccacacttca tcattaacga ttttcacttc ccgacccggc
     8281 agcggcgaac caacgtctgc cagaccgtcg gcttctttcg cacacactgt ggaggcaaac
     8341 tcggtcagac catagccgca aaagcaccga atcccctgct cgcgcgcctg ttccgtcaac
     8401 tcgaccggga tagccgcgcc gccaagtaaa accgctttca gggaaacgga actacggtta
     8461 accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc tgccagcatc
     8521 tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa ccagcgccac
     8581 ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc atcgtgatca
     8641 ccaaaaggaa tcagcgataa caccccttcg gcgctggcaa gatgagcctg ataagtatga
     8701 acagcggctt tcggcaagcc agtggaacca gaggttaacg tcattgagca cagacgcgtc
     8761 ggctgccacg ccgcggcatg tgcgccttca accagctgaa tgtgcagcga cgctaacgcc
     8821 ggaaacgtgt tttccccttc cggcaccagc gcaaattgca gcgtaagatt gggcagcaat
     8881 tcctcaagca gcggttgcgg cagctgaggg ttcacgggca acacccgcgc cccgcattgc
     8941 agtaacgcca gccaggccag cagcgtttgc ggcgtattcc acgcccgcaa catcacgctg
     9001 ctgccctcaa ccacaccctg caccgcaaag ctggaggcta attcatcgac gcgagcacaa
     9061 agttcgcgcc agttgagttg ctcgtcattg agacgtaagg cgatggcttc tccccgcact
     9121 tgccgccagt gacgccacgg ccagtcagag aagatcataa cagccgctcc agtgcatcaa
     9181 cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc tgcatcagat
     9241 ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc cgcgccagtt
     9301 gcgttagacc taagctcgac tcaatggaag agctgatcac cgccgtcagc cccagcgcat
     9361 gcgccgcctg cacttgctcg cgtactttat ccaggctgcc cgtgagcgtg ggtttgataa
     9421 ctaccgcgcg cacgccctct tcagctacaa aggcaaaatc cggctcgcgc aagctttcat
     9481 cccaggcaat ggcaatgccg gtttcacggg caaacgcacg cgaatcatcg cgggttttgc
     9541 acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacgtat ttggcaaact
     9601 gctggccttt cagcggtgtc caggcgcggt tcgcgtcaag acgcaaatgc agatccggaa
     9661 ttgcctccag caacagattc accaccatgc cgtcgcgcac ggcttcgtac aatcccactt
     9721 tgaccttcgc cactttctcg ccaggcatat cggcaagttt gaggatcaga tcgtcaggat
     9781 cgccattaca cagcggcgcc gcacggtaat tggccgcctg cgggagcgtc tctgccagct
     9841 ccgccaatgc acaacttacg ccaaaggcaa cggaaggcat ctgcggtatc tcgcaatcgc
     9901 ctgccagcca gttatttacc caggcaagca gcacactttg cgcatcttcc caggtttcct
     9961 gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgccc tcacgcaggc
    10021 aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccacc cccgcgtcca
    10081 tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt taaatttgct
    10141 gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt cttccgtcat
    10201 atagaacagc atggtggcgt tacctgccag ctcctgcaga cctgcctgac cgtcacagtc
    10261 ggcattcagc gcagctttca ggcagcgcag tgccatcggg ctgttttgca acatttcacg
    10321 gcaccagcgc acggtctctt tttccagatc cgccagcggt acaacggtgt tcaccaggcc
    10381 catatccagc gcctgttttg cgtcgtactg acggcacagg aaccagattt cacgtgcttt
    10441 tttctgccca acaatgcgcg ccatatagga agcgccccag ccgccgtcga aggaaccgac
    10501 tttcgggcca gtctggccga agatggcatt atcagccgcg atagtcaggt cgcacatcat
    10561 gtgcaggacg tgaccaccgc cgatggagta tccagccacc atcgcgacaa ccggtttcgg
    10621 acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc cggaatcatc
    10681 tttatagccg ccgtaatcac cacgtacttt ctggtcacca ccggagcaga acgctttatc
    10741 gcctgcacca gtcagaatga tcacgccgat gttgtcgtca taacgcgcat ccgccagcgc
    10801 ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg gacgattaat
    10861 ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa aaccttcgga
    10921 gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa tcatattgtg
    10981 tcctttagtc agaaacgcaa gatctgcgcc agacttgcga ttacgccagc gggattttcc
    11041 cggtgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag ttccgccgcc
    11101 agagcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa tgtgcgggcg
    11161 ctaaggttag cacgtaaatc gggctggacg gcgagagaag tcgcctccag catggcggca
    11221 agggttgcgc cattattgtt gctgcgcagc gccaccagct cccggcgttg atcgtcattg
    11281 agcgaggcaa aaacaggctg ttgataccaa tcggcaaata ccgccgttaa cggttctgta
    11341 cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg ttcagcattt
    11401 tgcaatccag gatgcccgcc ttcgacaaca actccacaaa gccccgccgg ctcctggcaa
    11461 gccgccatca tcgccacccg tccgccaagc gagtacccca ccagccagaa gttaagtatg
    11521 ttgtaactaa ccagcgtttt acacaataag ccggtgacat catcaaatcc atcgacgctg
    11581 atcgtcgctg aaccaccgtg acctgggaga tcaacataca accgcgagta gtcggcaaac
    11641 gcctcgccca cttcttgcca ttcatggcaa tcgccagaaa aaccgtgcaa aaacaccaac
    11701 cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct
    11761 gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc atttcaatca
    11821 ccgtggtggt cggcgtacgc caggcatcga ctagtgtcgt ttcaagttcc tgccagtttt
    11881 gcggacgatg atatttcagc tcgaacatcg cagcggcgtg ctcaaaatgg acgttttgcg
    11941 gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg caacagcgaa aaaatttgcc
    12001 cgccgttgtt gttcaccaca attaatacca gcggtgcgga aacctggcgc aataacgcca
    12061 gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc ggtttgccgc
    12121 ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg gcaccacggt
    12181 tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga cgcaccacca
    12241 ggctgttacc gacaaacaat tgcccctgtt caggcaagta gtcgctgatg cgatgcgcca
    12301 gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc tgttccgcca
    12361 ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc tccagccagt
    12421 cggcaatatt ggcaattaag cgacgtccgc gatggtgtgc cggatcaagt cgcccttcaa
    12481 tgtcatcaac aatccagtac tcttctggtt cacagcttgc ctgccattgg aggagtcgtt
    12541 tccccgtcag gctgcttccc agttgcacca ctatttgtgc ctgttgcagt tcgctggtcg
    12601 ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt tgcgacagca
    12661 cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt ttgccctctt
    12721 ccgcactcat gcgcccggca accaccacgc cgcgcttttg tcgccagaag aaccagtcac
    12781 gctgtttttc actttcccga cgaggcgctt cacgcagcca cggtttgtcg tcctgccacc
    12841 agtcgcccag ccgctgttgc cagctaatcc cggtgtcgtc catttcgcca tacagcggtt
    12901 cagcaaacgg gcagttgata tggactccac cagcatgaag cgtaccgaga gcgtggtcga
    12961 tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcagt gaaatactgt
    13021 gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg ccgcagtcaa
    13081 tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt aacccagctt
    13141 caatcagtgc cggatagaga tttgctaccg ccgtgccgga ggtcacaatc accgccaccg
    13201 gctgcttgct gactttcgcc agccccagtg ccagatgccc cagtccacgc tcatcgaaat
    13261 gggtatgatg aataaatgcg gaattctccg ccgccgctaa agttaacggt gtagaacgcg
    13321 agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc agaatgaccg
    13381 ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc aatattgcgg
    13441 ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga tgcgactcat
    13501 tactattcca tttgtaataa agtacgcagc cctgccgctt tgttgtcgat ttcctgccac
    13561 tcttgctcgg ggtcggaacc acggacaatg cctgcgccag catatagccg cacgatattg
    13621 ccgctgattt ttgctgagcg cagggaaacg cagaattcac tttgctgtaa tgagagatag
    13681 cccgcagaac cggcgtacca ttctcgagtg aacggttcgt gacgggcgat aaactgtcgc
    13741 gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg taaacagatc
    13801 acatcatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg tactttacgt
    13861 agacgcagta cctgcggcgg taaaacatcc agcgtttggg tatcggcctg caatcgttgg
    13921 cagatatctt ccaccaccaa catgttctcg cgctggtttt tatcatccgc catcagccac
    13981 tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc cgccagcgct
    14041 tcggtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga accaagaaaa
    14101 gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattgag ccgacgactg
    14161 gcagccatca tcgctgccgc attgaccgga cttgcgaaat gcaggtcggt tgcccgagca
    14221 agcacaactt tgtcgagctc gccttcggcg atggttttcg ttgccagttc gattaattgc
    14281 gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg taatccaggc
    14341 aggggcttga tactcaccag tgtggcgata aattcttttg ccttgattgc atcgtgctga
    14401 agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca acgtcgccat
    14461 tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcaa cccccagatg
    14521 cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc caacgacgta
    14581 aaacgggtaa tcgcacccag gacggcagct tcttcatcac cattacgttg ttgccagtag
    14641 aattgcggat acacctgctg actggccagc cagctcaagg catcaaaagc gtctttgaga
    14701 gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg
    14761 cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag
    14821 cctcacatta tacggggtac gacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa
    14881 cggcgtgcca gcaacagtcc cagaaccagc ccaacggccg cacccacacc aattccttgc
    14941 cagggttttt cgtggacgta gtcatcagca cgataaacag cctgcttcgc ccgatagtaa
    15001 taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt ttctgcacgc
    15061 gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag catctcttcc
    15121 agtgtttcac taagcagcgt caggtcatca tcaatacgtg tatcaccaaa ctgattagac
    15181 attctcgttc tccatttgtg taaaacctgt cactaactat agacaagggt tattgattac
    15241 gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc ataaatatcg
    15301 gtcacaggga tgaaaccaaa ttgcccataa aagttttgta aatgcgcctg cgcccccaga
    15361 taaatcggct tctccggcca gcggcgagta cacgattcca gcgccttact catcaattgc
    15421 tgaccgattt tttcaccgcg taaggcttcg ctgacaatca cccgaccaat cacaacgggt
    15481 tgcagttcgt cgtcactttt cagaatcctc gcatacgcca ccagtgtccc gttatgccaa
    15541 ccgagaatgt ggcgattttc tccctccaga tcgtcaccat cgatatcctg gtaaggacag
    15601 ttttgttcca ctacaaatac cgcacaacgc agttgtaata aggcataaag ctgagaaacg
    15661 gataaatcag aatgatgcag atcttgccag tcgatcatgg aaggctcctt tgtgctgttt
    15721 gctttatact agccggaata ttttcagcag cgcgatgaac tcatggaatt aattttttta
    15781 ggtacttcag ccggtgtgcc aacccgtacg cgtaatgtca cggcaatatt gcttaacctg
    15841 caacatccga cccagagcgg actttggtta tttgactgcg gtgaaggcac ccagcatcag
    15901 ctactgcata ccgcctttaa ccctggaaaa ctggacaaga tttttatcag tcaccttcat
    15961 ggcgatcatc tctttggttt acccggctta ctgtgcagtc gttctatgtc aggcattatc
    16021 caacccttaa cgatttatgg tccgcacggt atccgtgaat ttgtggagac cgcgctgcgg
    16081 attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt
    16141 ttcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact ggaatgttat
    16201 ggttatcgta ttgaagaaca tgataaacca ggtgcattaa atgcccaggc attaaaagct
    16261 gctggcgtgc cgccaggccc gctgtttcag gaattaaaag cgggcaaaac aatcatgctg
    16321 gatgatggaa ggcagattaa tggcgcagat tacttagccg ctccagtgcc gggtaaagcg
    16381 ctcgctattt tcggcgatac gggcccctgc gatgccgcgc tggagctggc taaaggcgtc
    16441 gacgtaatgg ttcacgaagc gactctggat atggcgatgg aggccaaagc caacagtcgc
    16501 ggccatagtt ctacacgcca ggcggcggca ctagcccgtg aggctggagt cggtaagcta
    16561 atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcagcacct gttacgtgaa
    16621 tgtcggtcaa ttttcccggc gactgaactg gcgaatgatt tcgcggtgtt taacgtttaa
    16681 ctctgcttat tattaaaaac agggcgaaac ttgccctgtt atcgcaaccc gcgctacata
    16741 tatccaccca cacgggtata ttgagcgatt ttttatattt attcatctcc tcatgcacta
    16801 tattcagagc tataagttgc ctctttttat cttgattcag caccaccttt gcaactaaaa
    16861 ggagcctatg atggttacag ttatcagcaa ttattgtcaa ttatctcaaa cccaactctg
    16921 tcagacattt gcagaaaaat ttactgtgac cgaggaatta ctgctgtctt taaaaaaaca
    16981 ctcataactc attacaaata atgatgaaat aacgcttttt attgataatt tacatatgaa
    17041 aaaaatgaca tctctttgtt ttataccatt actactatcg cttactttta ttatttttta
    17101 ccaaccctgg gtcagggcat taccaccaac gccccgacac gctagtcctg agcaattaga
    17161 aaaaacagtt cgttatctta cacaaactgt gcatccacgt agcgccgaca atattgataa
    17221 tctgaatagg tccgcagagt atataaaaga ggtctttatc agtagcggtg ccagagttac
    17281 cgcgcaggat gtccccatta cgggtggccc ctacaaaaac attgttgcta attatggtcc
    17341 tgccgatgga ccgctgatta ttattggtgc gcactatgac tctgtcagta gttatgaaaa
    17401 cgatcaattg acctatacgc cgggcgcgga tgataacgcc agcggcgtgg caggattact
    17461 cgaactggca cgtttgttac agcagcagac tccagggatt ggcgtccaac tggtcgccta
    17521 tgcgtcggaa gaaccgccct tctttcgtag cgatgaaatg gggagcgcgg tacatgcagc
    17581 ttcgcttgag cgtccagtga aattaatgat agcgctggag atgattggtt attacgactc
    17641 aacgcctggt agccaggatt acccatcccc ggcaatgtcc tggctttatc ccgatcgggg
    17701 agactttatt gccgtggtcg gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc
    17761 ggcattgtta tcatcccggg atttatctgt ttattctatg aatgccccag gttttattcc
    17821 cggtgttgat ttctctgatc accttaatta ttggcaacac gatattcccg ccgtaatgat
    17881 tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata tcgcagacag
    17941 attaaattat cagaaaatgg ctcaggtagt ggatggagtt acaaccttgt tatacaacag
    18001 taaataataa aaatttctcg aggcggatcc agaaaaagca acatgcgcat tttattttct
    18061 ttcgtctgtt ttaagatgcg aaacatgaaa ttcaacggag gaaaaataaa tgggaatgat
    18121 tggctatttt gcagaaattg actcagaaaa gattaaccaa ctgcttgagt ctactaaaaa
    18181 acctttgatg gataatatac atgacacgct ttcaggatta cgccgactgg atattgataa
    18241 acgctgggat tttttacact ttgggctgac aggcacctct gcttttgatc ccgcgaagaa
    18301 tgacccgctg agtcgcgccg tgcttggtga acacagccta gaagatggca tagatggctt
    18361 cctgggattg acctggaatc aggagttagc cgcgacaatc gaccgactgg aatcactgga
    18421 tcgaagtgag ctgcgtaaac agttttccat taagcgactc aatgaaatgg agatttatcc
    18481 gggagtaacc ttttcagaag agttagaggg gcaactcttt gcttcgatca tgcttgatat
    18541 ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt
    18601 tattgttggg tgatggtgtc agggcaccaa aaaaaaccgc cgaacatgtc ggcggctttc
    18661 tgacttacaa actgactgtt tacatcagcg gcattgctaa ccgcacaatg ctaatcagcg
    18721 gttgtggcca tacacccagc accagtacca acagtgcaga gatcagcacc acgataccgc
    18781 ttgcgctgta ctgccagttt gatggtgcat cgcgacccgg ttgttccgga gcgtgcaggt
    18841 ataagctcac cgccacgcgc aggtagtagt aaaggccgat tgccgaacca acaaccacgg
    18901 cacccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga
    18961 taaagcccag cgtcattggg ataccagcca gtgaaagcat catcaccgtc atcactgccg
    19021 cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc
    19081 cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat
    19141 aaccgaccag gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca
    19201 gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatat
    19261 tggtctggct cagcgccatc aggttaccga agatgataga ggcaaaggcg ataatcgcca
    19321 gcactacgcg aatcgcttca ctgtcaccca ccggtgcgta aaggaacaaa cgcatcacca
    19381 cgccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc
    19441 cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg aaaccgaggc
    19501 caacaatcat caggccaaaa cctgccagca acagcggctc gttgagcata ccgtcgccga
    19561 ggtttttacc cagtgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac
    19621 cgaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca
    19681 gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt
    19741 cgataccgag gaacagagac gccagatggt tggcattcgc cagcaggatc ccgcccagcg
    19801 cggcaattaa caccaacagg tagaactcgt ccttgttgtc gttatagcct tcaagccacg
    19861 ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg
    19921 cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc
    19981 agagcgatac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat
    20041 tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg
    20101 gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag
    20161 taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa
    20221 tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca
    20281 gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga
    20341 agtaagcgcg atgcagcatc gccagcgaat aaacagaagc aaagaccagc ccaaaggtag
    20401 agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga
    20461 cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg
    20521 gcagccattt cattttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac
    20581 gttcataaag ctgaccacaa agaataaaca gacccgccgc cgacaagccg tgcgcaatca
    20641 tctggattac cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga
    20701 agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg
    20761 ccatccacgc accatagaag atgccgataa cacccagcca catagcgatt ggcgcgaact
    20821 ctgccgacgc gttcgggaac agcggtaggg agaaacgcag cagaccgtaa gcggcggttt
    20881 tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg tgcctgggag tgcgcatccg
    20941 gtaaccagcc atgcagcgga accaccggca ttttcactgc gaaggcgatg aagaagccca
    21001 gcatcaacag gtattccacg ccgttagaca tcggcgtatt cagcagctct tcatagttga
    21061 aggtccagac gccggtcgca ttgtagtgaa cgaaaaccag cgccaggatg gcgatcaaca
    21121 tcaccagacc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt
    21181 taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca
    21241 tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc
    21301 ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg
    21361 aacagagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc aacgacagac
    21421 cgtcaatggc gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag
    21481 actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt
    21541 gcagcgacag cgccagcgtc aatcccatgg ttaccagcgc gatccagcgc ggcaccttga
    21601 cgccaaagcg ttcggtctgc cagcacagga agccgccgat aaagggaatt aatattagcc
    21661 agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa
    21721 cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag
    21781 acggcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa
    21841 cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac
    21901 cagcgcagat aaccgttctc gcttaacagc agacctttac ctgcaaagcg ggaaaggaca
    21961 gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg
    22021 aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag
    22081 gtacccagca gacggcccgg cgcactgttg gcgatggagg tcaccagagt acgtttaccc
    22141 agccacagcc aggcagccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc
    22201 agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc
    22261 ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg
    22321 tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa
    22381 atcatacgga aggtgtaaag cgaggtcata aacgcaccga ccagacctgc caccatcaga
    22441 ttgatatgtc cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca
    22501 gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc
    22561 ggaatagatt tacgcagacc acccatctta aagatgttct gttcgtgatg gcaggccaga
    22621 atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgggt catcaagtgg
    22681 aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc
    22741 atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgccgc aaaaccggcc
    22801 agcagcagcg taaccgcccc aacaatgccc accagatgca gaacttccgg cgtcatcagg
    22861 aacagaccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg
    22921 atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc
    22981 aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg
    23041 ttattgccgt cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg
    23101 cccagttcgt tgtaaagaat gaacagcgcg aaagcgagga agacgtcacc cacacgggtc
    23161 acgacgaacg ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc
    23221 agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttgtcg
    23281 gcaagcacca gaactaccat gctggcgatg aacaggttgg tgtaagcgaa gaagcgagag
    23341 tagccctctt caccgcgcat ataccaggag gcgtacatgt gaataaggaa acccacacca
    23401 gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta
    23461 aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgcctgctc accgttagcg
    23521 aagaagtcaa cgccgataaa ggcagttacc agcgccgcca ggcccacaga gccaacgccg
    23581 acgatcgccg agacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca
    23641 atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat
    23701 caatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg
    23761 cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctggcccc
    23821 agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa
    23881 tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga
    23941 ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt
    24001 cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccaacgtg
    24061 gaaggccaca accagacccg cgagcagcag catagaagcc aattccaccg ccagtacgta
    24121 aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg
    24181 atcgttaaca ccgagaatgg cgtaaacaat caccaccagc atgatggctg acaaaattgc
    24241 cggaccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc
    24301 caggttgagc atcatcacta cgaacacgaa cagcaccata atggcacccg catagacgat
    24361 aatttccagc gcaccggcga agtaagcgcc cagtgagaag aacaccccag agatcgccag
    24421 cagcgaaata atcaggtaca gcagcgcgtg taccggattg gtatgggtga tcactcgcaa
    24481 ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct
    24541 tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct
    24601 ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga
    24661 ccggagatca gcagatcctc tttctcgtaa accagatcct ggcgcttgta ttcccccatt
    24721 tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag
    24781 aaaatgcagc gtgagaagtt gatgcggaaa aattccggat accagcgacc gtctttggtt
    24841 tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct
    24901 acgcaacgct cttcgccgtc cggatcgcgg gtcagaacga tacgaccacg ataacggggc
    24961 ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg
    25021 ccgatcatcc agatactacg aacctgggta ccgaaaccta ctaacaattc ttttaaggtc
    25081 atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt
    25141 tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg
    25201 gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg
    25261 cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca
    25321 tcagtgcaga gatggtcaca atcccgatgt attcgcccac gaagaacaga ccgaacttca
    25381 taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa
    25441 acgggtgacg gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt
    25501 gcgggatgac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac
    25561 cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct
    25621 gcgcagaagc acgcatcgcc cccagcagcg agtatttgtt gttacttgac cagcccgcaa
    25681 acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca
    25741 ggtcggcaac cacccaaccc ggactgactg gcacaattgc aaaggccagc agcagcgagg
    25801 taaaggcaat catcggtgcc agggtaaaga tgacacgatc cgagaatttc gggatccagt
    25861 cttctttaaa gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa
    25921 cacggttagg tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca
    25981 tgaatgcccc acaggtgact accaccagca ggatcaccac cgctttaagg acggtcagca
    26041 ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag
    26101 atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc
    26161 tgctgtcagc ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa
    26221 ggagacgcgt gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat
    26281 gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc
    26341 aaacaggtgg tagtacggcg cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg
    26401 tacgctggtg aagtaatcca gaccgttttc gctggtttca aacagacgca cgcccggatc
    26461 gccaaaacgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt
    26521 ccagcctggc gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc
    26581 ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct
    26641 gatattggcg cgcatggcgg tacgaccgct atagcgatgc ggttcacggg ccagtttctg
    26701 cccacgaata cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat
    26761 tttcgccaca acagcgtcaa tcacgtggtc gagctgcgtc cagtccactt cacggctcag
    26821 cagggtgcta tgcagcgagt gcagccagcg ccagctttcc agcatgacag tttggctgtc
    26881 gtaataagcc ggatcgtaaa cttggaagaa acgttgggcg cggccttcgt tgttgatcac
    26941 cgtaccgtcg ctttcagcaa agctggcggc ggaaagtacc agatgagcgt tttccataat
    27001 cgctgtgcgt tgatggtcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac
    27061 gcgggtagcg gaagcgtgac gatgcagatc gttttccagc accaccaccg cgtcggcgcg
    27121 tccggtttcc agttcagtta acgcttcttc aagcgatcca ccgcctataa tgcccagccc
    27181 catgctgttg acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag
    27241 ggctttcgcc acgttagccg cagcctgaat cacttcagcg ctaccagcgt tcgtcccgga
    27301 gataatcaac ggtttcttcg caccagccag tgcctgcacg atgacgtcga ttttgctttg
    27361 cagctcaggc tctatgccgt caactgccgg tgcggagttg tccagcgcat gggcgatggc
    27421 aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc
    27481 cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt
    27541 gaggattgcc gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt
    27601 accttttacc gcctggcgca ccgccagcgc aacgcgcgcg ccggtctggg taacgtcttc
    27661 gcccagcacc agtaccgcat cgtaagattc gatttcacgc agagccggag tataaatgcc
    27721 gccttcacgc agtactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc
    27781 ggtatagaag ttttcttcgc ccaccagctc acgcagcgca aagttgcttt ccacgctggc
    27841 acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg ctgcaccctg
    27901 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg
    27961 cggacgatct ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta
    28021 gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccat aacgctcgcc
    28081 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
    28141 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggtg aatacgccgg tcgggcaaat
    28201 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
    28261 gacgttgtcg tgcgcaccgt aaacgcccag gtccgtaccg tcagcgtaat ctttgtagta
    28321 acgcacacag cggtagcagg cgatgcagcg gttcatttcg tgagagatga atggccccaa
    28381 atcctgatta cggtgggtac gtttagtgaa acggtagcga cggaagctgt gtccggtcat
    28441 cacagtcata tcctgaagat ggcagttacc gccctcttca cataccggac agtcgtgcgg
    28501 gtggttggtc atcaaccact cgaccacact ttcacgaaac tgtttcgctt cttcgtcgtc
    28561 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
    28621 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
    28681 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
    28741 caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag
    28801 catgcttcca gttttctcag tcagagactg agcgttaatc gaaattcggt taccagcgct
    28861 ctttcagcag gtttggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga
    28921 ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
    28981 caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg
    29041 tttcgatatc gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc
    29101 acggcagacc gtcgcggcat ggcgtacacc agccgcagga ctcacgggcg aaaaactctt
    29161 ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg
    29221 tacccagacg gcttcccgct ttaccgatac tttcgaattc catcggcagg tcaaggtgcg
    29281 cttcggtcag gaagtcagtc cccgctccgc ctggctgcca ggctttgaat ttcagaccgt
    29341 cacgcatacc accggcgtag tcttcgagga tctcgcgtgc ggtggtgccg aacggcaatt
    29401 cccacagacc cggatttttc acccgaccag agaagcccat cagcttggtg ccagcatctt
    29461 tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac
    29521 acagggtttc gacgttgtta acgcaggttg gtttacccca tgcaccggag gttgccggga
    29581 agggcggttt cgagcgtggg ttagcgcgac gcccttccag ggagttgatt aacgctgttt
    29641 cttcaccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaaa tcgaaacctg
    29701 ttcccataat gtttttgcca agcagacccg cttcggtggc ttcggcaatg gcacgacgca
    29761 gattcactgc cgcttcgata tattcgccgc gcaggaagat atagccacgg taagctttca
    29821 gcgcaaacgc ggagatgagc atgccttcca ccagcaggtg cggcagttgc tccatcagca
    29881 gacggtcttt ataggtgccc ggctccattt catcggcgtt acacagtagg taacggatat
    29941 tcatggattc gtctttcggc atcaggctcc acttcaggcc agtcgagaag cctgcgccac
    30001 cgcgcccttt cagaccagag tcttttacct gattaacgat ttcgtccgga gacaacccgg
    30061 tcagcgcctt acgcgcgcct tcgtaaccgt ttttgctgcg atattcgtcc agccacactg
    30121 gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt
    30181 ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt
    30241 gtcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag
    30301 cagcgtgaag cgaccgtcaa atgtcgtttg ccctggtttg atgttcagtt ttttctccag
    30361 cgccgcctga ataccctggt aaccgttgat gtgacagacc acgctgtcgc aataacggat
    30421 cacatggcgg cctactggct gacggaagat ctggctataa aacgttgcca cgccttcgac
    30481 gtcgctcgcc ggaataccca gcacatcggc gatcgcgtgg atcgcgccat ccggcaccca
    30541 gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta
    30601 gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt
    30661 ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata
    30721 ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc
    30781 tgcaaatgcg cataactcgg cgtacggata cgggtgcgat aactcatggt gctgccgtcg
    30841 ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa ccatctggaa ggattcattg
    30901 gcaggcatca ccggacccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt
    30961 tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct
    31021 tccggcatgt tgttgaggca ctgctcaaga atacgcagac tctggcgcaa ctcttctact
    31081 ttcagcatta cgcgggtgta gcagtcagaa acaccaccgc ccaccggaat ttcgaagtcg
    31141 aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtggca
    31201 cgcaggcccg cgccggtggt gccccactcc agcgcctctt tcgcgccgta agcggcaacg
    31261 ccctgggaac gacctttcag aatggtgttt tgcagcgccg ctttctcgta agacgccaga
    31321 cgtttcggca tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg
    31381 tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac gaaaaccagt gattgcttcc
    31441 accagatcgt aaattttctg acgatcggta aaggcgaaga acactggcgt catcgcgccg
    31501 acgtcctgaa taaaggtgga gatatacagc aggtgactgt tgatgcggaa cagttcggag
    31561 agcattacgc gaatgacgtt aacgcgatcc ggcacggtga tcccagccag tttctctacc
    31621 gccagcacat aaggcatttc gttaacgcag ccgccgaggt attcgatacg gtcggtatac
    31681 ggaatgtagc tgtgccagga ctggcgttcg cccattttct ctgcgccacg gtggtggtaa
    31741 ccgatgtctg gtacgcagtc gacaatctct tcgccatcaa gctgcaaaac gatacggaaa
    31801 gcaccgtgcg cagacgggtg gttcggaccg aggttgagga acatgaagtc ctcattttcg
    31861 gtgccgcgct tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc
    31921 tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta
    31981 cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg
    32041 tcgaatgtaa tgccaaatag atcccaggtt tcacgctcat accagttagc gttcgggaat
    32101 aatttggtaa aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg
    32161 tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac
    32221 ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa
    32281 ggtttcggca gtttctttaa gaaatcgccc acttccagta attgttcacg cttgatccac
    32341 acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg
    32401 cgcagttcgc caatcaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc
    32461 gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag
    32521 taagcttcgc gaaatagatt taggaataag cgaagaaaat tccctttgcc gacaggcgca
    32581 aattaaatct cgtcaggtgt acgcaggttg gtaacggcaa tgcgttcacc gcgcttgcgt
    32641 tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc
    32701 ggacgacgtt ctttaccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca
    32761 ggacgcggcg gacagcccgg gatatacaca tcaaccggga tgaatttatc aacgccctgc
    32821 acaacggaat aaatatcgta cataccgccg gagttggcac aggcacccat tgagataacc
    32881 cattttggtt ccagcatctg gtcatacaaa cgctgaataa ccggtgccat tttggtaaag
    32941 caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttcagcg
    33001 ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag
    33061 gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg
    33121 agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc
    33181 tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa
    33241 tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc
    33301 atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa
    33361 accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg
    33421 ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg
    33481 aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag
    33541 tcgataccgg attcaaacgg aacgtttttt gacctcgcgc gtgcgcgacc gcctaaaaac
    33601 caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat
    33661 gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa
    33721 gtagcgcctg attctctgct ctttacggca gatggacgcc acatcgattc atggggagga
    33781 ataaaaaaac cttacaataa ctgcagaaac tattttatac agctaattga tgtggtcttt
    33841 tactcctttc tataaccttt tgtcaacttt aacaaaggtt tcttcacact gatttacaca
    33901 atgacaacaa catttgcatt tggcgccctt tctccccaga tcattgacga ctccaggaaa
    33961 attagatgtt gttgaatcgt gtccgttgtg aagcaatgga aaaaatacgg gtctattttg
    34021 acaggaattt gtgtcgattc ctccccccaa aagagagtat tttcttgatc tgtgacacgc
    34081 ttttgtcatt ctataacaaa aatgcagcaa caaattcacg tattttttaa cattattgta
    34141 gcaggagatt tttttcaggt gattatttgt gcgtacgggg cgtgaatctc tggtgattga
    34201 aaaatgaaca gttttgtacg ttctgcacga tacgaggaag gctttttact aaaaaaaagc
    34261 cgctggggtt taaaataaac ccagcggttc gtttttacac tattgtctca ggaattatct
    34321 atcgtccgtc gattactcga tatccctttc aatcaacaat gagtcatctc cttccggagc
    34381 agacattggg ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg
    34441 ggccagctca ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg
    34501 cggtaagcca tctactccac tcagcacacg caggtccgga ctcatcatct cgaccggcct
    34561 tgccgtcacg ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg tcgatgcgac
    34621 ataagccagt cgccagggaa tatctgcttt attaagcgtc gccagcacga tatcgcgaaa
    34681 cggacttgga tcatccagca acacaagagg gatcggctca cctttttgca atacgtattc
    34741 cgctgcgcag taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgttgaagg
    34801 acgatgggtg gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc
    34861 attacgttta acgcggacat ccagcgctag tttcggataa accgaactca cgcgatttaa
    34921 caggaaaggt aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg
    34981 aagattactg aacattaatg atgagcaggc ctcatcatta aaacgcagaa tttttctggc
    35041 gtaaccaaga agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa
    35101 cagttctttc ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt
    35161 acgacacaca gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt
    35221 tctcagcaga tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca
    35281 cttactggcg gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat
    35341 ttgtaatgtg cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg
    35401 agttgcacta aaaaagtgac tcacattgtt ccgttataat gcctgaagta gatcacagaa
    35461 tatatcttca gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg
    35521 cgtgtttgta taaatgtaaa tgtgagtcct tgttccactc tcgtacagca tcgctggtca
    35581 tacgcgaaca cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt
    35641 atgcaagcat t
//
LOCUS       DAZFRE010000047        35260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFRE010000047 DAZFRE010000000
VERSION     DAZFRE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.047"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..443)
                     /locus_tag="ACHKUI_002625"
     CDS             complement(<1..443)
                     /locus_tag="ACHKUI_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6598801.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLP"
     gene            complement(701..1954)
                     /gene="amiC"
                     /locus_tag="ACHKUI_002626"
     CDS             complement(701..1954)
                     /gene="amiC"
                     /locus_tag="ACHKUI_002626"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6598802.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2186..3517
                     /gene="argA"
                     /locus_tag="ACHKUI_002627"
     CDS             2186..3517
                     /gene="argA"
                     /locus_tag="ACHKUI_002627"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6598803.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3579..5405)
                     /gene="recD"
                     /locus_tag="ACHKUI_002628"
     CDS             complement(3579..5405)
                     /gene="recD"
                     /locus_tag="ACHKUI_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6598804.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTDAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5405..8947)
                     /gene="recB"
                     /locus_tag="ACHKUI_002629"
     CDS             complement(5405..8947)
                     /gene="recB"
                     /locus_tag="ACHKUI_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6598805.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMKGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHLFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIVWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLIPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8940..11828)
                     /gene="ptrA"
                     /locus_tag="ACHKUI_002630"
     CDS             complement(8940..11828)
                     /gene="ptrA"
                     /locus_tag="ACHKUI_002630"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6598806.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMSADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDTLKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12004..15372)
                     /gene="recC"
                     /locus_tag="ACHKUI_002631"
     CDS             complement(12004..15372)
                     /gene="recC"
                     /locus_tag="ACHKUI_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6598807.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQFTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15385..15708)
                     /gene="ppdC"
                     /locus_tag="ACHKUI_002632"
     CDS             complement(15385..15708)
                     /gene="ppdC"
                     /locus_tag="ACHKUI_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6598808.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHSWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15693..16100)
                     /gene="ygdB"
                     /locus_tag="ACHKUI_002633"
     CDS             complement(15693..16100)
                     /gene="ygdB"
                     /locus_tag="ACHKUI_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6598809.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQMQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16097..16660)
                     /gene="ppdB"
                     /locus_tag="ACHKUI_002634"
     CDS             complement(16097..16660)
                     /gene="ppdB"
                     /locus_tag="ACHKUI_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6598810.1"
                     /translation="MSVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16651..17121)
                     /gene="ppdA"
                     /locus_tag="ACHKUI_002635"
     CDS             complement(16651..17121)
                     /gene="ppdA"
                     /locus_tag="ACHKUI_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6598811.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17306..18100)
                     /gene="thyA"
                     /locus_tag="ACHKUI_002636"
     CDS             complement(17306..18100)
                     /gene="thyA"
                     /locus_tag="ACHKUI_002636"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6598812.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18107..18982)
                     /gene="lgt"
                     /locus_tag="ACHKUI_002637"
     CDS             complement(18107..18982)
                     /gene="lgt"
                     /locus_tag="ACHKUI_002637"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6598813.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19133..21379)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_002638"
     CDS             complement(19133..21379)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_002638"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6598814.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21392..21922)
                     /gene="rppH"
                     /locus_tag="ACHKUI_002639"
     CDS             complement(21392..21922)
                     /gene="rppH"
                     /locus_tag="ACHKUI_002639"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6598815.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22275..22421)
                     /gene="ygdT"
                     /locus_tag="ACHKUI_002640"
     CDS             complement(22275..22421)
                     /gene="ygdT"
                     /locus_tag="ACHKUI_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6598816.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22607..23296
                     /gene="mutH"
                     /locus_tag="ACHKUI_002641"
     CDS             22607..23296
                     /gene="mutH"
                     /locus_tag="ACHKUI_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6598817.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23365..24078
                     /gene="ygdQ"
                     /locus_tag="ACHKUI_002642"
     CDS             23365..24078
                     /gene="ygdQ"
                     /locus_tag="ACHKUI_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6598818.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAI
                     FFSIAVESLNLIRNKKNPL"
     gene            24216..24434
                     /gene="ygdR"
                     /locus_tag="ACHKUI_002643"
     CDS             24216..24434
                     /gene="ygdR"
                     /locus_tag="ACHKUI_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6598819.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24542..25582
                     /gene="tas"
                     /locus_tag="ACHKUI_002644"
     CDS             24542..25582
                     /gene="tas"
                     /locus_tag="ACHKUI_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6598820.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25614..26807)
                     /gene="lplT"
                     /locus_tag="ACHKUI_002645"
     CDS             complement(25614..26807)
                     /gene="lplT"
                     /locus_tag="ACHKUI_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6598821.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26800..28959)
                     /gene="aas"
                     /locus_tag="ACHKUI_002646"
     CDS             complement(26800..28959)
                     /gene="aas"
                     /locus_tag="ACHKUI_002646"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6598822.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29545..30576
                     /gene="galR"
                     /locus_tag="ACHKUI_002647"
     CDS             29545..30576
                     /gene="galR"
                     /locus_tag="ACHKUI_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6598823.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30583..31845)
                     /gene="lysA"
                     /locus_tag="ACHKUI_002648"
     CDS             complement(30583..31845)
                     /gene="lysA"
                     /locus_tag="ACHKUI_002648"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6598824.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31967..32902
                     /gene="lysR"
                     /locus_tag="ACHKUI_002649"
     CDS             31967..32902
                     /gene="lysR"
                     /locus_tag="ACHKUI_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6598825.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32889..33581)
                     /gene="ygeA"
                     /locus_tag="ACHKUI_002650"
     CDS             complement(32889..33581)
                     /gene="ygeA"
                     /locus_tag="ACHKUI_002650"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6598826.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRLGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33710..35128)
                     /gene="araE"
                     /locus_tag="ACHKUI_002651"
     CDS             complement(33710..35128)
                     /gene="araE"
                     /locus_tag="ACHKUI_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6598827.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     RALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKMGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8706 a         9610 c         8663 g         8281 t
ORIGIN      
        1 tcaggaagtt gaacgctgaa ttgctcatta atgatagtta atgcatcgcg acttatcgca
       61 tactctgctt tgtacaacgc tttgatttca tcaaggaaaa cattattaat ttgtatacct
      121 gaaacgactc gggatattgc attcgaaatg tgatccgcaa gaatcaatag tagcgatgga
      181 ttgagatttt tttcgagatt tttctccgca tattgcacta ttttttcggc aacaaacaca
      241 tactcaatat ctacatgttc aatcaattta taaagtttgt ttttttgttc atttctgaca
      301 taaaaaatcc ggttagccgg atgctcaggg accgacattc catatttttt gttgtaaccg
      361 acgccaggcc cagaaatgat aacctcctgt ccattcatcg atgcctgtac acaattattg
      421 ttcatgactt tttcgatgat cattccatgg ctccaaaaaa aaaggcaagg cacgtcagaa
      481 gtaataacta cttcaaacat tgccttgcct gatttaacag ttacaagatg attaactaat
      541 gcgtatacat tacacggaat tgtgcttaat taaagagatg gtcggcttat ttttcaagcc
      601 gacattcaaa tatccgacat cagtcacaaa accagcgtct aatattcagg taaatgttat
      661 ttattcaacg aaatataaat tcattgaata aataaaaaca ttatcccctt ctcgccagcg
      721 tcgccccatc ggcaaaatac gctttaattc ccgcaagaat agactccgca acttcctgct
      781 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
      841 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
      901 ttttatgcag cttgttgatt ttaccgagct tattcagcac cgctttacca aacttaaggc
      961 tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
     1021 cgcttttgct tacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
     1081 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgatgaaccg ctcggctggc
     1141 gactggtaaa ggcgtcggca tggatagaaa caaacaagtc agcgcgctgt ttctgggctt
     1201 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
     1261 tattgccctc tttctcgatc agagagcgca gacggcgagc tatttgcaat accacgtctt
     1321 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
     1381 gcataatgac aatcggacga tcccgcccag ctttacccgg ttgtggacca ctttgtgctg
     1441 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
     1501 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
     1561 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatac
     1621 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtcagcgc
     1681 ggatttgcgc agccatcccc ttgagcaccg agttcaggtt tacatcttcg atatccacca
     1741 ccacgcgttc aggattactc aacgcgaact gcttatattt cagctgacga tttgattcta
     1801 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg
     1861 cagccaggct gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac
     1921 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
     1981 gctgtgtaac aagtaaacga ccaatttgac cggaaacttt agcgaaagac gcataatctg
     2041 tcatctataa aacggtaaac attcttttta tattcacggt attactgata aaaaactcgt
     2101 tcccgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
     2161 gaataatcat gcaaagaggt gtaccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
     2221 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
     2281 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
     2341 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
     2401 tctggctgca catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
     2461 aacactggaa ctggtgaagc aggcagcggg aacattgcaa ctggatatta ctgctcgcct
     2521 gtcgatgagt ctcaataaca caccgctgca gggcgcgcat atcaacgtcg tcagtggtaa
     2581 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgtc atagcgggcg
     2641 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
     2701 aatggggccg gtcgctgttt cggtcactgg cgagagcttt aatctgacct cggaagagat
     2761 tgccactcaa ctggccatca aactgaaagc tgaaaaaatg attggttttt gctcttccca
     2821 gggcgtcact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
     2881 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
     2941 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
     3001 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
     3061 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat atcggcggta ttctggagtt
     3121 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
     3181 ggaaatcgac aaattcacca ttattcagcg cgataatacg actattgcct gcgcagcgct
     3241 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
     3301 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
     3361 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
     3421 atttacccca gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
     3481 gcgtaaatcc aaagtgttga tggcggattt ggggtaagcg atgaaaatcg tcggatgcga
     3541 catgcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
     3601 gccgccagac cactgcgccg ctcggtgcgc gtggcaattg ccgcacttaa aatgcgctca
     3661 tcggcataca gcgacagacg gcgacgcgcg cgggtcactg cggtgtaaac cagctctcgc
     3721 gttactaccg gcgtgcgttg actcggtaaa atcaacgccg catggtcgaa ctccgatccc
     3781 tgcgatttat gtaccgtcat cgcccacgtc gtttcgtgct ctggcaggcg actcggttgc
     3841 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
     3901 agcgcaatac caatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
     3961 actggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
     4021 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
     4081 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
     4141 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
     4201 tcgccactct gtaaaagccg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
     4261 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
     4321 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcgtctgtg
     4381 ccagttcctg ccggaacgtg agttcccgtc aggcggctta gctgcccggc acgctcggcg
     4441 gtaaagccgg cattggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
     4501 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
     4561 agtctcgaca tcataggaag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
     4621 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc acccagcaat
     4681 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaat
     4741 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
     4801 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagtaacttc
     4861 gctacggtgg tcgttttacc ggtgccagga ccaccggaaa tcaccgagat ccgccgtgtc
     4921 agcgccactg ccgccgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
     4981 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ttcgttgaaa
     5041 aagcgtgcca ctgtgcgctc gttacaccac atgcgattca aataaagacg atcgccacag
     5101 aggatcatcg gcgttggttc atctccccgg ctgaccgctt gtgaagccag caagcattct
     5161 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgatgcc
     5221 tcgttatttt ccagtcgtga aagcggcaaa caaacgtgtc cctctccggc atcatgactt
     5281 aatagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaac
     5341 tgcacgtcca gtgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
     5401 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg tcaatcaacc
     5461 cggcgttggg tcgggtcgcg tagatccctt gttgcggatg ttctttatca acgccacgca
     5521 ggaacagata aataacgccg ccaaagtggc gctcatagtc gtaatcagca atacgatggc
     5581 gcagataacg gtgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
     5641 gcattgccgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc aaccagttgg
     5701 atttatagtc gagcaggtaa taacgccctt cgtggcggaa caccagatcg ataaagcctt
     5761 ttaacatgcc acgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
     5821 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatt ggcaggtaaa
     5881 actccatctc cacctgttta tcgcgatcgg aaagctgatt caggctaaca cccgtttcat
     5941 tgagaggtgc ctggaggaca gccgtgatcc actcagtcaa caccggttcc cactgcggtt
     6001 caaagccacc gagctccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
     6061 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
     6121 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
     6181 gtggtatcag atcctgagcg ataccgtgac cacgctgctg caaaccggag tagctggtga
     6241 cacgccagtt atcgccgggc aatcgttgta gcgtcctcgc attcagttct gcagttaatg
     6301 catcattaac ctgccagggc tggttatcac caatttgtgc cgtttgccag acaatatcat
     6361 cattgcacaa cgcttcaata caggtacgaa gccctgccgc atcttgcggt tctccttttt
     6421 gcagcaaacg cccgagcgca ctttggtgga cgtcggtatc acctttttta tcgccacgac
     6481 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
     6541 aaagtaaacg cagatcttcc gccagacgtt cggcctccgc gaggtcgacg ctttctggcg
     6601 cagcattaag atccagaacg gcctcaaaca agtggcgatc gtgataaaac gcctgatcct
     6661 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc ccttttgatt
     6721 tgtgtatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
     6781 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
     6841 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagac
     6901 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
     6961 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
     7021 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
     7081 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
     7141 ccgcctgcaa cagccaaagc atctcctgtg cttccagagt ttcaaaaaca ctgtcgcggt
     7201 tcgaaaggta aacggaaggg atttccagca gcgttaaggc atcgcgcacc tgagcggcct
     7261 cctggcggct gcgcaccagc acactgatgt ccgaagcacg caccggacgc gcatcgtcgc
     7321 cgttcatcag caacgcttcg ccgcgttgcc cggcttgtaa ccagtcgcgg atttgcgcag
     7381 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg cctttcatca
     7441 gccacatttt catcgcaggc tgcgtttcac ctttaaacac aaaacgtaac gcctgatttt
     7501 tcccggctga tttcacagga ataaacggta tttcacgaaa catgaacgcg tcatcagtct
     7561 ggctgaaaag tttgttaacg ctgttcacca ttcccggtgc ggaacgccag ttggtatcta
     7621 aggtgtagtg agcatgaact tcgctacgcg ccttcatata agtgaagata tccgcacccc
     7681 ggaatgcata aatggcctgt ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
     7741 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
     7801 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa tacctcgccg ctttcactac
     7861 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
     7921 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg agtgatcacc agatcgcgga
     7981 tcgacaatgg ttctgcaagc agttgttcga tcgcctcaaa cagtggatgt cgcggggttt
     8041 cccccccggc tttcgtgcga tcttctaaga aacgctgaga gaatttttcc agcgactccg
     8101 gcaactgata gctgttggtc tcttcttctg cccaggcgct gatcttctcg atccatttag
     8161 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
     8221 gttcacccac tgcgtcgcgc cactgctgtt ttacggcatc aatacgcgcc acaatttgcg
     8281 cgtggcggga agccagcgtt tcatcatcgg ggggcggtgc tttgataacc ggcgcttcgc
     8341 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
     8401 cgacctgggc aatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
     8461 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
     8521 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
     8581 catccatctg ccgctcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
     8641 cttccagcag gcgtttgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
     8701 actcgtggat attgctacgg atacgaccac gcaattctgc cgtagcagcc tcggtaaagg
     8761 tcaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcagaa ccgcctagtc
     8821 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
     8881 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
     8941 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
     9001 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
     9061 gacagaatag ccataccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
     9121 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
     9181 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
     9241 agcatctgag taattaccgc ctgctggatt tgcgcaaact cctctggctt catcgcccgc
     9301 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
     9361 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccctacgct cattgggaac
     9421 gcaaacacgg catagcccaa ctgttcttcg gtacgcaact gattgtagaa ccacggctgt
     9481 acgatctgcc ccaacagaga actatacgct gagctggtgt attcatcgta gccagtcggt
     9541 acaaataccg ctgccagtgc ggagtcggtg ctgttaccag ctttttcaaa gatgacggat
     9601 tgttttttat cgaccacgac atctttgtta cgacaccact ctgaaccatc agcgcccaac
     9661 tgtttttgca catggcgtgc cagcgttgtt gcctgggctt cagtcatgtt gccgataacc
     9721 ataaactctg gtctagcccc tgattttaag gtgtcgcgat aggccagcac ctctttcaac
     9781 gtaatggagg gcaaaatttt gcgccgttca tctcgcgaga agtacggcac ttgcgagagc
     9841 atctgcgcgg gcataatcgc ctgctcaaac gctttgccct tttctgcgga atccatcatc
     9901 tggttatacc aggacttcgc ctgctcaagc tgatcttccg tagcggtata gctaaagtag
     9961 ccctccagca gagcctggaa cagctgcggc aggcgctgag tgtaaccatt ggcattaacc
    10021 ataaggccgt tgttagcatt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    10081 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    10141 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    10201 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcaactct
    10261 ggatggtcgt atttcttctc tgacttaatc agcgagaaat cgtcaggaat ataggggtta
    10321 agctccggta aggagagcgc aatgttggcg gcttttttct gccagtccgc gaaagtttgt
    10381 gcgctaattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    10441 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttct
    10501 tttactgctt tagcatcgta ccgatcggca atattgactg catccagtgt atgctcaaca
    10561 ggaacgcgaa tcatggtatc agccagccat tcgacgtaat ccatatcacg ggtgattgac
    10621 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcgatc
    10681 cctttttcac gtaacagatt gaggtagcta aaaatggccg ccacaacctg atcgcgattc
    10741 gccagacctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    10801 ataggatcgg aattggcgct aataccctca actaatccct gcttttgcaa ccagtcagaa
    10861 agtgtacctg ggctgcgatt accaatcaga taggtaatca attcatccgt tttactacgg
    10921 aacttcgctg aattgttatc gatgcgaaac tcaacgcgca gaactttacg cggcaacgcc
    10981 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    11041 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgacat tttcgccaac
    11101 tccggcaacg gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc
    11161 tcgtggaaat ctttcagcgc ctgctgtacc ggattgcctg gtttgtcgct taaagtttcg
    11221 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    11281 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcggcatt cactgcatta
    11341 cgttcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcgtc cgccaggcgg
    11401 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggt
    11461 gccgtgctgg cattgtgact gccgccgtgc attttgagat attcggccag actgtcagcc
    11521 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccttgg
    11581 tacgcctcag gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    11641 gcctgcggat cagaaaccag taagaccacc ataccgttat ccagacgtat agcctgatac
    11701 tggcggttat ctttatcact tttacggatg gtttcctgaa tgggctgcca tcccgtttct
    11761 gcctgactta agggtgccca aagggcaaca aacaacaata atgctttgaa ccagatgctg
    11821 cggggcattc acggacctca taatcaactt tattttctgg ccagattcaa cacgttaacg
    11881 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtc tatataacca
    11941 tcagtccgtg actggtgcgc atcataaagt aagcggatgg attgcgcaat ttttatacaa
    12001 tactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    12061 agcctccatt gtctctggtg tgaattgccg ccagagccgt tgataccaga tatcatcacc
    12121 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    12181 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    12241 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    12301 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    12361 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    12421 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    12481 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    12541 atcaatctcc atactctgcc ctggctggcg acaggcaatg actctgtcgg caagttgctg
    12601 catctcctga cactgcgttt cccagaaaat ttcaccaaag gcaccatacg gcaaatcacc
    12661 tgccgcccgg aatcggcgga acaagcgttc ggcatcatcc tgctcaacca gtacatttaa
    12721 taactgctgg ttgatttgat aacgactaag cccttcaaga ataaaaggtt cagtgtcggg
    12781 aatttcgctg tcttcggtac ggaagttcac ctgcaaacgc atctggaaaa acgcccgtac
    12841 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaatgg
    12901 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    12961 acgagcatag ctttgtcgtt cgcctggctg gtagttttgt ggatcaaacg gcattcgggt
    13021 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    13081 atcgcccggt agataatggc tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    13141 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    13201 ctgcgcggaa attaacgctt ccaggaacag atagcggtcg tcatcgcgac ggctacggtc
    13261 gccacgcttc ggtttctggc tcatcagatc aaagcccaat ggcgcaagct gacgtggata
    13321 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    13381 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    13441 gcgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    13501 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ctgcttcggt
    13561 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    13621 cagcggacgc tcctgcgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaaga
    13681 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    13741 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atgcgcgtca ggccaaaccg
    13801 ccaggtgtgt tgaccggtag cgggaagttc cagctcgcga acgttgtcgt catctatccc
    13861 ccaacgaatg ccggactcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    13921 gtcaaaccgc gctgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa
    13981 gcggctgtct ggcagcgata acaggctgat aaacgcttca agtacaggat gcgactgccg
    14041 cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    14101 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    14161 agtaagtgtc gggtcttcct ccagcatcgc cagcaggcga tcgtgtaaaa cttcaacttc
    14221 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    14281 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcgc ggttttccag
    14341 ttccagaatg tcagactgaa tattatgcag caggttatct ggcgtcacat cgacaaaagc
    14401 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    14461 gccccatgaa gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    14521 cccggcattt tcgctgtcgc gaaatagcgg taattcgcga tcttcaaaac tgtgtcggcg
    14581 ctggcgagtc agtagtttcg ccagataagc gggatcttta atgtcgcccc agtaataacg
    14641 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct gtagcgcctg
    14701 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    14761 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg
    14821 ccagcgcggt tgcccaagtt cgtcggtata ttccaccaac gccttccaca acggcgcttg
    14881 ccaggcctgt gcttctccca acccttctac cagatgtcct gtttcccact gtgccagcca
    14941 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgaag aaagctggaa
    15001 cagttttcgc ttgtcactat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    15061 ctccaacagt tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaagc
    15121 gctctctttg gggatctccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    15181 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    15241 ctgtgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    15301 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    15361 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    15421 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    15481 tgtctgcatt cggttgacct gccagttagc aggtggcgaa atcgcgcgca gttgcgtttg
    15541 ctgccagcta tgccgccaga gctgttggta ctggtttctg ctggcaagac tgttcattaa
    15601 tgttcgctgg aaacccgata acgcagtgac aatcatcacc atcaacacca tcgccaacat
    15661 tacctccggc agactaaagc cttgttgatt cctcagggaa gctgacataa cgccctctct
    15721 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcgatgact
    15781 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    15841 gccagtaaac gcagacaaac ccgggcaccg gtggcagcat acagtaagca ctgaactgcg
    15901 ggctgcatct gccaggagtg catttttccc cactccagcg ccgactgaac gatggcctgg
    15961 cggcgcaatg actgactttc catgctcacg cgagaagcaa aactgcgatc ctgctgactc
    16021 attccttgca atagcaagct acccaaaatc agcagcatca ggaccagagc cagtgacgaa
    16081 acaccctttt cgcggttcac aggttggatc ctgtcacgct atagctggca tccaccacgg
    16141 tttgcggatc agccttgctg gcagcgtgca tattaaccgt caacaccggc gagaagccgc
    16201 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    16261 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    16321 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccattac
    16381 tgttcgcatc ccactgcaca atgacacagt caccctgtcc gacaatttcc agcccttcgc
    16441 ccgtacagct gccatggcaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    16501 gcagccagat ttcatcttcc agcgccagct tacgggtgtt cgttaaactt tcacgctgta
    16561 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    16621 acacttccag cagagaaaaa ccttgctctt ttacagacat ccttctgttt ctccttgctg
    16681 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    16741 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    16801 caggtcgctc atttcgactt cgggccagcg cggcacaaag accaatggtg aactgccatg
    16861 acaggtattc gccccagcaa cggaactcac aaggcaccat aacgtcccct ccctgataag
    16921 actgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    16981 ccgcgcctgg ctggcagttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    17041 gagcccactt gcgcttagca tgaccagaat cagcatcgcg accagcgttt caatcagcgt
    17101 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    17161 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    17221 tttcgggaag gtgtctcgaa gaatttaacg gagggcaaaa aaaaccgacg cacactggcg
    17281 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    17341 gatcgtatcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    17401 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    17461 ccatatggtt gctgtagaga tgcgtgtcgc caccggtcca gacaaaatca cccacttcca
    17521 gatcgcactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttgaacggca
    17581 ggccgaggaa gacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    17641 catagaactg gaagaatgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    17701 tccacgctga aacaataatg cgacgggaat ccgggtcgtt tttcagctgg ttcagtaccg
    17761 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    17821 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    17881 cgtgtagata agcaatgtta gtgtcgccct gcaggaacca cagcagttcg tggatgatgg
    17941 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    18001 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    18061 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    18121 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    18181 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accagtaaac tgcgcgtcgg
    18241 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta accaatcagg aacaagcctg
    18301 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    18361 caccttccag cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    18421 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    18481 cagggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    18541 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggggca ataaaatcag
    18601 agacctggaa gaaggaacgt ttagtacggc gggcgaagat aatcatcacc acgataacgc
    18661 caatcaggcc gccgtggaag gacatgccgc cgtcccagac acggaaaaga tacagcggat
    18721 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtcca ccgaggaaga
    18781 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccactgcccg
    18841 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    18901 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    18961 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    19021 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    19081 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    19141 ccccgcgaat cagcccgccc atgccgcgac gctccataaa ggcagcaacc tgatggcgaa
    19201 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcagcaaaat
    19261 caatgcgccg cagcaggtat tttacgcgcg ctacagaacg tccgttcata gacagatggc
    19321 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    19381 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    19441 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    19501 ggatgtattg cgtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    19561 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    19621 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    19681 cgtcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    19741 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    19801 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    19861 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcatta aacatttgca
    19921 gcatcccctg atactgcgcc acctgttctt cttccgacgg aaaaccactt tgcagcatga
    19981 atgggatttc agtgcgataa agtcctatgc catcaatacg gctgcccagt ttttcttcat
    20041 gttccgggct taaaccggca ttgagcatga ctttaatgcg ttcgccgctt tttaactggg
    20101 cgggtaaatt gacgtcatct tccgccagac ggctaagctc gatctcttca ctaattagcc
    20161 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgataaccat
    20221 cgacgatcag cgtccgacga tgcagtaccg aaggctgaat atccgcgccc atcacggtag
    20281 gtatccccag cgcacgtacc atgatcgcag catgggagtt ggcagcgcca tcgcgcacga
    20341 caacaccgac taagcgatcc tggggcagct cagcaagcgt tgtcgctgac agttcatctg
    20401 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    20461 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    20521 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    20581 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtatcc gaaagcaggt
    20641 gtgagtaaag atcgaaaata gccgccgttt ctttttgtgc accggcagca aagcgtttgc
    20701 tatagcggcg aaactcgtta gccgcctctt ccaacgcccc ggtcagtcgt tcgcgttcca
    20761 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    20821 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcacggatt cgcgtctggc
    20881 gatattgccc aaacaaggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    20941 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    21001 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    21061 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    21121 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    21181 ttacagtgcg accacgtggt tttttcagtc cccgggtcgc catcaggtag taacaacgtc
    21241 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    21301 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    21361 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    21421 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    21481 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgactgga taccagtaac
    21541 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    21601 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    21661 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    21721 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    21781 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    21841 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    21901 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    21961 gtttcatact ccagtaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    22021 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    22081 tattcacctt ttctgtggat agagttgtga agaactacgg aattgctacg ggaaaacccg
    22141 gagcattctg aataagacag atatagagat tttaaaatat tcattatcag tcaatgcatt
    22201 acatgtgatt tgagtaataa aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac
    22261 gtgttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    22321 actgaaccac taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt
    22381 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    22441 tttcagtgac cagatttgga aaacccgttg cagtgttgcg caactcgttt accggcaacc
    22501 taaaaagcaa tataaatcag agaattgaac aacgcatgtg gaaaaagtta cactgcgaat
    22561 attcagcaca taattgctgt ttgtttttta accaaggtat catgacatgt cccaacctcg
    22621 cccactgctc tctcctcccg aaactgaaga acaattgtta gcgcaagcac aacaactttc
    22681 tggttataca ttgggagaac tggcggcact tgccgggctg gttacgccgg agaatttaaa
    22741 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    22801 taaacctgag caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag
    22861 tcttggtcgt ccactggaaa caacattcgt ttgtgttgcc ccgttaacgg gcaatagcgg
    22921 ggtgacctgg gaaaccagcc acgtgcgcca caagctcaaa cgcgtgctgt ggataccggt
    22981 tgaaggcgag cgcagcatcc cgctggcaaa gcgtcgcgta ggatcaccgt tgctgtggag
    23041 cccgaatgaa gaggaagacc gacagttacg cgaagactgg gaagaattaa tggatatgat
    23101 tgttctcggt caaattgagc ggatcaccgc tcgtcacggg gaatatttac agatacgacc
    23161 gaaagcagcg aatgcgaaag cgctgaccga agctattggt gcccggggcg aacggattct
    23221 gacgctgccg cgcggatttt atttgaagaa gaatttcacc agtgcgctac tggcccgtca
    23281 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    23341 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    23401 cttgcgctcg gtacactgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    23461 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    23521 ttggcgggag ccatggttat gcgtctggcg ctgctggcat ccatcgcctg ggttacgcgc
    23581 ctgacgaatc cgctttttac aatattcagt caggaaattt ccgcccgtga tttgatcctg
    23641 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccatcgaa
    23701 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgcgat cgtgcagatt
    23761 atgctgctgg atattatctt cagcctcgac tcggtgatta ccgctgtggg tctgtcagat
    23821 cacctgttta ttatgatggc tgccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    23881 cgctcgattg gtgattttgt cgagcgccat ccttcggtaa aaatgctggc gctctctttc
    23941 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    24001 tacatctact tcgcgatatt cttctctatt gcggttgaaa gcctcaacct gattcgcaac
    24061 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactcctgt
    24121 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt tataaacaga
    24181 ctattatcat aggtgggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    24241 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    24301 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    24361 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    24421 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    24481 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    24541 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    24601 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    24661 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    24721 aactcaaggg ctaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    24781 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca aaggcatccg
    24841 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    24901 tctgcagact gattacctcg atctctatca ggtgcactgg ccgcagcgcc caaccaactg
    24961 cttcggcaaa ctcggttata gctggacaga ttctgcgcct gcggtttcgc tgctggatac
    25021 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    25081 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
    25141 tattgtcacc attcagaacc cctacagcct gttaaaccgc agttttgaag taggtctggc
    25201 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
    25261 actgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
    25321 tcggttcacc cgctatagcg gtgagcagac gcaaaaagcc gtcgcggcgt atgttgatat
    25381 cgccagacgt catggcctgg accctgctca gatggcgctc gcatttgtac gccgtcaacc
    25441 gtttgttgcc agcactctgc tgggcgcaac cacgatggag cagctgaaaa ctaacgtcga
    25501 aagtttgcat ctggagttga gcgaagacgt attagctgaa attgaagcgg tgcatcaggt
    25561 ttatacgtat cctgcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga
    25621 cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc gaagccaatg
    25681 ccaatgggca cgaccgggat gcctaccatt actgccagcg agtaaatgcc cagcatcaac
    25741 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    25801 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    25861 ccaatcagca tcaacaaagc ataggctggc agcagctcgt gttgcaggga gaaaatcagt
    25921 accactacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaact
    25981 aacttcgctg ccgcacctgc accaaccacg ataccaatcg ctaccatcgc gttgagatag
    26041 gtcggcgtag cgttatcggt aatacccagc gccaccggta cccacagcac caacaggaaa
    26101 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    26161 ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgct gatgagattc
    26221 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgcg
    26281 ccaccgtagg ccagtgcgca tgcggccagg gcaaccagaa tatgccagtc agccagaaca
    26341 ccaccagcta cggaaccgag caaaatcgcc gctatggtag acgcttccat taaaccgtta
    26401 gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    26461 taggctgcag caccaacacc caccaacgta tagccgagaa acggattgat accaaagcag
    26521 atactggctg cgcccagtag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    26581 aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc
    26641 atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg
    26701 gcaaacagta gcgcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    26761 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    26821 ttcgtctacc cagcttttca aagtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    26881 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    26941 atactgttgt aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    27001 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    27061 tgccagttgt tccaccattt ccagcgacac catttcacct gcaattttgg caaagcgttt
    27121 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    27181 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    27241 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    27301 gccctcttcg ataccaggaa ccgacaacag gcgcgcgtcc atacctggta gaatacgccc
    27361 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcactcggt
    27421 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    27481 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    27541 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    27601 gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    27661 tgcacctgtg agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    27721 taacgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    27781 gttcgccaga atgcttttat ggctatggac gacgcctttc ggatggcctt cagaaccgga
    27841 ggtaaaaagg atcagcgcct cttcttccgg ctgctgtttt acctgtgcca gacgcggcat
    27901 cagcaaatga gcgaaaatcc acactttgtc ggcagtggtg acatctgctt ttaaatcttc
    27961 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    28021 gagaaactga cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    28081 cccttttacc ccggcggtgt agttcatcat tgcgggaatg cggcgacggg cgatggcccc
    28141 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    28201 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    28261 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    28321 tgcactcagt aaagattcgt acagcgtttc acgcgggcgc accgccatac gcgcttccat
    28381 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    28441 cggcatctcc acctgcgttg gtggcaaaat atgcagagta atttgcggga acaagcgacg
    28501 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    28561 aataaccgtt gcaccagact tcgccgcgac aaatcccgcg ccatcgtaga ttttcatcag
    28621 cgagccagtg gtggtgatgc gtccttcagg gaaaatcacc actggtcgtc cctgttccac
    28681 cagacgtacc agatgtttaa tcgccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    28741 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaatac
    28801 cggacgtacg ggtaaaaaca atgccagcag tatgccatca ataaaagaga cgtgattagg
    28861 cgtaattaga acgcgctcgc ccttcagtgc cttggtgtca cccgtaacgc gaacgcgata
    28921 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    28981 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    29041 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagactggg tacgaagagc
    29101 gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct tcacaacgac
    29161 tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt
    29221 cggttactgt tacagattga tgaccgacaa aaaaaaacct gcgcatctgc gcaggctggt
    29281 gtaattcatg tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact
    29341 ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta
    29401 accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac
    29461 aatgaaaaca cgccgcccct tgaaccaacg ggcgttttcc gtaacactga aataatgtaa
    29521 gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc
    29581 gtttcagtcg ccaccgtttc ccgtgtcatt aataattcac ccaaagccag cgaagcttcc
    29641 cggcttgctg tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt
    29701 gcgctggcgc agcagaccac tgaaacgatc ggtctggtcg ttggtgatgt ttccgatccg
    29761 tttttcggcg caatggtgaa agcggtcgaa caggtggctt atcacactgg taatttttta
    29821 ttgattggca acggttacca caacgaacaa aaagagcgtc aggccattga gcaactgatc
    29881 cgccatcgct gtgctgcgct ggttgtccat gccaaaatga tcccggatgc cgatttagcc
    29941 tcgttaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa
    30001 aaccgttgta ttgctctgga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt
    30061 cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa
    30121 gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattccggc caatgaccgg
    30181 ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg
    30241 ggacgaggca gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggcgcg
    30301 atgggcgtgc tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt
    30361 gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc
    30421 gtgacgatgg cgacgcaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc
    30481 ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc gccattcagt gtcaactccg
    30541 tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc
    30601 cagtaattct tcaatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag
    30661 aacttctggt aacagcggac ggctgttgta gttggatgac attgatgcgc catatgcccc
    30721 cgtatcatgc agtaccagat aatcacccgc cttcacttcc ggcaaggcgc gggtttcgac
    30781 atttccccct tcctgctggg taaagacatc gcccgattca cataacggcc cggcgacgac
    30841 ggtttccacc gttggtgcgt gttccagaga acgaccatca gctgccaggg cactgatatg
    30901 gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat caaccagcac
    30961 aaagtggcgg ctacccattt gtttgacgct ccgcacttga gtaattaata cgccagcctg
    31021 cgctaccaga aagcgacccg gttcaatttc cagtttcaca ggatggccca aatggcgggc
    31081 gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc
    31141 accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta aatcctgacc
    31201 gaactcgagg acctgacgca ccatagcacc acatacctgt tccagatggg cataatcgac
    31261 gccagaacca atgtgcatgt gaatgccgac cagctgtaga tgatgacgtt gtatcacgtc
    31321 cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt
    31381 ggttttttgg ctatgcccgt gaccaaaccc cggattaaca cgcagccata cccgatgccc
    31441 tggcgaaacc tgacccagtt ggtcgagcat atcaacagaa cccgcattca ccggaatttg
    31501 caattcactg actcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc
    31561 atcggggtgc gtttgcggat tgtaacccgc cgccagtgca cgctctattt cgcctaacga
    31621 gacggaatcc acttttacgc cctgctcacg cattaagcgc aaaatatgaa tattggaaca
    31681 ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat
    31741 aatttgcgca tcgtagaccc acaccgggca gccaaattct gcgggcaaac gcagcagatt
    31801 ttcggcggtg agatcggtat cagtgctgaa cagtgaatgt ggcataacaa actccagata
    31861 agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga
    31921 gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa
    31981 cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcggc
    32041 acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt
    32101 gatcgggctg aaattgtttg agcgcatacg tggacgatta catcctaccg tgcaaggact
    32161 gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagtgccgc
    32221 agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc
    32281 gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt
    32341 aaacatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga
    32401 tttaggactc actgaaacgc tccatacgcc tgcgggaaca gaacgtaccg aattactctc
    32461 tttagatgaa gtgtgtgtgt tacctccggg ccatccgctg gcggtaaaaa aggtattaac
    32521 gccggatgat tttcacggtg agaactacat cagcctttcc cgtactgaca gctatcgcca
    32581 gttgctggat caattgttta ctgagaatca ggttaaacga cgcatgatcg tagaaaccca
    32641 cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggcgtttcgg tggttaaccc
    32701 gctcaccgca ctggattatg cggcaagcgg tttagtggtg cggcggttca gcattgcggt
    32761 tccattcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggtgcaggc
    32821 gtttagtgag catttacaag cgggattacc gaaactggtc acttctcttg acgctatttt
    32881 gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcggtatca
    32941 aacacaggca gaacactgcg ctcttctggc accagtaaac caatttctgt gcagccaaaa
    33001 atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt
    33061 gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt aatttttgcc
    33121 cgttcatccg cttcaggaat aagacagttg atggaaaatt gttccgtcag ccgcccgcga
    33181 taaaaatcct gttccatggt gtaacgtgta cccagcagcg ccacacgagt cattcctgcc
    33241 ccggtaattg cacgtccagt ggcatccgct atgtgtaaga aaggcagaga gcaacgagac
    33301 tcaatggcat ccgccacttt gtgcatcgta ttggtgcata acacaatacc ttctgcgccc
    33361 gcccgctgta agccaagcgc cgcctcagcc agaatatccc cggttttgtc ccactcaccg
    33421 agacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag
    33481 tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta aggaatagtg
    33541 gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg
    33601 ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcctaatc
    33661 tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga
    33721 tatttctcaa cttttcgcct gccatcagtt tgcgctcgat atgttccagc gtgacatttt
    33781 tggtttccgg aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga
    33841 gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggtc gcgccgataa
    33901 tcatattcga cacccagttt gtcgtcgttg agcaggtgat gccaaaatcg cggcatttca
    33961 gcggctgaat ttcagagcac aagatccaca ccactggcgc ggcgctcatc gcataaccgg
    34021 caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt
    34081 tatcaaactg catcaggcag taacccagca ccagagtgcc taacgccatc acgctgaaac
    34141 ccattttcag agccggctta cgccctgctt tatctaccgt aaagaccgca atgaaggtag
    34201 cgaacataaa ggtcagcccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa
    34261 agcccgccat tttgaagata cgtggcgcgt agtacatgat gatgttcata ccggtaaact
    34321 gctgcatcgc ctgcaacaac ataccgagaa atacagcacg acggacgtta cggttgatct
    34381 taaacaatgc ccaaccgccc tgttttaact tcaggctttc acgaatttcg ttgagttctt
    34441 ctcgcgcttt ttccgacgta tcgcgcagca tacgcaatac ttcttccgcc tcaatatgac
    34501 gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca
    34561 gaactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg
    34621 ctgtatcgga taaaaacgcc agcacgatgc cgagagtgac catcaactgg tacatgctga
    34681 tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atatagagga gcggtgtaag
    34741 acgcgatccc gacagcaatg cccaacacca cacgagcggc gattaacatc tctacgctgg
    34801 tcgcaaaagc ggaccctatc gaaccgagta caaacaggat ggcccccgcc atcaggctgt
    34861 atttacgccc caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga
    34921 gcatcatgct actgaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa
    34981 tgaacggcaa cgctcgggcg attacgccga tatcaagacc aaataacaat cctgcgaccg
    35041 cagcagctac cgaaacaaac atattcatac gccgcgtatc ccgcagagga cgtggcgtta
    35101 aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg
    35161 aaaaaatacg tgaacaattc acacaggtgt caggtcggaa atagcataaa tatggattaa
    35221 attgctacga catgtcgtta tgtgatggat attccaattt
//
LOCUS       DAZFRE010000048        35237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFRE010000048 DAZFRE010000000
VERSION     DAZFRE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.048"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            529..1617
                     /gene="pdeL"
                     /locus_tag="ACHKUI_002652"
     CDS             529..1617
                     /gene="pdeL"
                     /locus_tag="ACHKUI_002652"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO6598828.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1659..2591)
                     /gene="yahB"
                     /locus_tag="ACHKUI_002653"
     CDS             complement(1659..2591)
                     /gene="yahB"
                     /locus_tag="ACHKUI_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6598829.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLITRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2683..3180)
                     /gene="yahC"
                     /locus_tag="ACHKUI_002654"
     CDS             complement(2683..3180)
                     /gene="yahC"
                     /locus_tag="ACHKUI_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6598830.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3438..4043
                     /gene="yahD"
                     /locus_tag="ACHKUI_002655"
     CDS             3438..4043
                     /gene="yahD"
                     /locus_tag="ACHKUI_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6598831.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDHTCLNPFLMSCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4083..4946
                     /gene="yahE"
                     /locus_tag="ACHKUI_002656"
     CDS             4083..4946
                     /gene="yahE"
                     /locus_tag="ACHKUI_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6598832.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIVLPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITIGQHHHIEMSHCQRWLSPTMQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLALILFLPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4936..6483
                     /gene="fdrA"
                     /locus_tag="ACHKUI_002657"
     CDS             4936..6483
                     /gene="fdrA"
                     /locus_tag="ACHKUI_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6598833.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSAEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6483..7900
                     /gene="yahG"
                     /locus_tag="ACHKUI_002658"
                     /pseudo
     CDS             6483..7900
                     /gene="yahG"
                     /locus_tag="ACHKUI_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
     gene            7924..8004
                     /gene="yahH"
                     /locus_tag="ACHKUI_002659"
                     /pseudo
     CDS             7924..8004
                     /gene="yahH"
                     /locus_tag="ACHKUI_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            8054..9004
                     /gene="yahI"
                     /locus_tag="ACHKUI_002660"
     CDS             8054..9004
                     /gene="yahI"
                     /locus_tag="ACHKUI_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6598834.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9014..10396
                     /gene="yahJ"
                     /locus_tag="ACHKUI_002661"
     CDS             9014..10396
                     /gene="yahJ"
                     /locus_tag="ACHKUI_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6598835.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGGAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFKCEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10613..10687
                     /locus_tag="ACHKUI_002662"
                     /pseudo
     CDS             10613..10687
                     /locus_tag="ACHKUI_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10774..11823
                     /gene="yahK"
                     /locus_tag="ACHKUI_002663"
     CDS             10774..11823
                     /gene="yahK"
                     /locus_tag="ACHKUI_002663"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO6598836.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADRINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12445..12688
                     /locus_tag="ACHKUI_002664"
                     /pseudo
     CDS             12445..12688
                     /locus_tag="ACHKUI_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12705..13379)
                     /gene="yahN"
                     /locus_tag="ACHKUI_002665"
     CDS             complement(12705..13379)
                     /gene="yahN"
                     /locus_tag="ACHKUI_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6598837.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCGEIFSLIRIVGGAYLLWFAW
                     CSSMRRHSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPT
                     WARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASWVIGAIIGVFALRLIY
                     EGVTQR"
     gene            13526..13801
                     /gene="yahO"
                     /locus_tag="ACHKUI_002666"
     CDS             13526..13801
                     /gene="yahO"
                     /locus_tag="ACHKUI_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6598838.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(13902..15488)
                     /gene="prpR"
                     /locus_tag="ACHKUI_002667"
     CDS             complement(13902..15488)
                     /gene="prpR"
                     /locus_tag="ACHKUI_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6598839.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQASETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            15727..16617
                     /gene="prpB"
                     /locus_tag="ACHKUI_002668"
     CDS             15727..16617
                     /gene="prpB"
                     /locus_tag="ACHKUI_002668"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6598840.1"
                     /translation="MPLHSPGKAFRAALSKETPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            16777..17946
                     /gene="prpC"
                     /locus_tag="ACHKUI_002669"
     CDS             16777..17946
                     /gene="prpC"
                     /locus_tag="ACHKUI_002669"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6598841.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            17980..19431
                     /gene="prpD"
                     /locus_tag="ACHKUI_002670"
     CDS             17980..19431
                     /gene="prpD"
                     /locus_tag="ACHKUI_002670"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6598842.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYETAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNSADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            19471..21357
                     /gene="prpE"
                     /locus_tag="ACHKUI_002671"
     CDS             19471..21357
                     /gene="prpE"
                     /locus_tag="ACHKUI_002671"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6598843.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALAMIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            21682..22941
                     /gene="codB"
                     /locus_tag="ACHKUI_002672"
     CDS             21682..22941
                     /gene="codB"
                     /locus_tag="ACHKUI_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6598844.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            22931..24214
                     /gene="codA"
                     /locus_tag="ACHKUI_002673"
     CDS             22931..24214
                     /gene="codA"
                     /locus_tag="ACHKUI_002673"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6598845.1"
                     /translation="MSNNALQAIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLETGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(24335..24946)
                     /gene="lacA"
                     /locus_tag="ACHKUI_002674"
     CDS             complement(24335..24946)
                     /gene="lacA"
                     /locus_tag="ACHKUI_002674"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO6598846.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRIIREINDRDKQYYFKDFKVESSV"
     gene            complement(25012..26265)
                     /gene="lacY"
                     /locus_tag="ACHKUI_002675"
     CDS             complement(25012..26265)
                     /gene="lacY"
                     /locus_tag="ACHKUI_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6598847.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATVEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(26317..29391)
                     /gene="lacZ"
                     /locus_tag="ACHKUI_002676"
     CDS             complement(26317..29391)
                     /gene="lacZ"
                     /locus_tag="ACHKUI_002676"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6598848.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLERDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQSEAALLQCTADTLADVVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(29514..30596)
                     /gene="lacI"
                     /locus_tag="ACHKUI_002677"
     CDS             complement(29514..30596)
                     /gene="lacI"
                     /locus_tag="ACHKUI_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6598849.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSWLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(30633..30731)
                     /locus_tag="ACHKUI_002678"
                     /pseudo
     CDS             complement(30633..30731)
                     /locus_tag="ACHKUI_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            30798..31337
                     /gene="yaiL"
                     /locus_tag="ACHKUI_002679"
     CDS             30798..31337
                     /gene="yaiL"
                     /locus_tag="ACHKUI_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6598850.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNNEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(31563..32396)
                     /gene="fghA"
                     /locus_tag="ACHKUI_002680"
     CDS             complement(31563..32396)
                     /gene="fghA"
                     /locus_tag="ACHKUI_002680"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6598851.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(32489..33598)
                     /gene="frmA"
                     /locus_tag="ACHKUI_002681"
     CDS             complement(32489..33598)
                     /gene="frmA"
                     /locus_tag="ACHKUI_002681"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6598852.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(33633..33908)
                     /gene="frmR"
                     /locus_tag="ACHKUI_002682"
     CDS             complement(33633..33908)
                     /gene="frmR"
                     /locus_tag="ACHKUI_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6598853.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(34097..34870)
                     /gene="yaiO"
                     /locus_tag="ACHKUI_002683"
     CDS             complement(34097..34870)
                     /gene="yaiO"
                     /locus_tag="ACHKUI_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6598854.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENTTLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNNPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(34872..>35237)
                     /locus_tag="ACHKUI_002684"
     CDS             complement(34872..>35237)
                     /locus_tag="ACHKUI_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6598855.1"
                     /translation="IGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNH
                     RLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVE
                     RNLPTGTYSLRQELIRTGD"
BASE COUNT         8674 a         8996 c         9365 g         8202 t
ORIGIN      
        1 gtcattgtta taatgttggc tatctattgc tgaatggatt cagtcttaaa aagcaggttg
       61 ttaagggata ggcatagagt aatgacgtgt atgcataacc aacatcttta ctcattatgt
      121 cattgaatgt tgacgctatg tgtttatgag gttgaggtat tttcagttga tctggattgt
      181 taaattcata taatgcgcct ttactcatga atgggcgtca atacgtagtg ggaaattata
      241 aatatcgaaa tagtccaact acttctttat taccaaaaat gagtatctga attttaatat
      301 tgcattcttg cgtgattatc tcctgagttt gacttgtgat taccttttta aggtatttag
      361 cgtaactgtt tttgagcgag catcagaggt aaagataatc ttcttgatag tgatgtggga
      421 tgttatacgt atggcatcgc taatgtttat ggttacccct tatgtgtgct caggaatcca
      481 caggtaatca ctcatactga acagcgataa aagataaaag gtgtgttcat gaattcatgt
      541 gattttcgtg tttttctgca agagttcggt acaacggttc atttgtcatt gcctggtagc
      601 gtatccgaga aagaacgact gctactcaag ctgctgatgc agggaatgtc tgtaacagaa
      661 atatcacagt acagaaatcg cagtgcaaag accatttcac atcaaaagaa acagctcttt
      721 gagaaactgg ggattcagag cgatattact ttctggcggg atattttctt tcagtacaat
      781 ccggagatca tatccgctac gggtaataat agtcacaaat atattaatga taatcactat
      841 caccatatcg tcacgcctga agccatcagt ctggcgttgg aaaaccacga gtttaaaccg
      901 tggatccaac cggttttctg cgcgcagact ggggtactga caggctgtga agtgcttgtc
      961 cgctgggaac atccacaaac gggaatgatc ccaccggatc agtttattcc tctggcggag
     1021 tcatccggtc ttattgtcat aatgactcgc cagctgatga aacagactgc ggatattctg
     1081 atgccggtaa aacatttgct gccggacaat ttccatattg gtatcaacgt ctcggcgggc
     1141 tgttttttgg ctgcgggatt tgaaaaagag tgtctgaacc tggttaagaa attaggtaac
     1201 gataaaatca agctggttct tgagctgacg gaacgtaatc ctattccggt aacgccagaa
     1261 gccagagcga tatttgacag ccttcatcag cacaatatta cctttgcgct ggatgacttt
     1321 ggtacgggtt atgcgaccta tcgttacttg caggcgttcc cggtcgattt tattaaaatt
     1381 gacaagtcgt ttgtgcaaat ggcgagtgtc gacgaaatct ccggtcatat tgtggacaat
     1441 attgtcgaac tggcgcgtaa gcctggtttg agtatcgtgg cggaaggggt agaaacccag
     1501 gagcaggcgg atttaatgat cggtaaaggc gttcactttt tacagggcta tttgtactct
     1561 ccgccagtac cgggtaataa atttatctct gaatgggtaa tgaaagcagg tggttgatgt
     1621 aaaccgctat tcacagcgca tcgggaggtt ggcagcgatt aattctcccg atgcagtaaa
     1681 tcctgataga tccccgttaa tatgccattt ggcgcaaatt gttttttaat ccactgcgtc
     1741 acctgaccgg tggcagagtg ctgggttgct agtaacatgc gtgaatcctg gcgcgggtta
     1801 tggatttgtc gggtaatcag caaggattgc gccatcgcct cacggaccat gtaatccggc
     1861 aaaaaaccaa ttccttcacc taaaatctgg cactgacatt tggtgttgaa atctggcacc
     1921 agaattgact cctgcccatg cagcaaccag cccacttttt tattaatcgt atgcgcggtg
     1981 tcctccacca tgatattagg gtagagacgc agctggcttt cggcgatcgg ctccggcaca
     2041 aaggctaatg ggtgatccgg ggcgatggca aaagcccagc gaatcgcgcc aatctccgtg
     2101 taatcaatgc cgccgccgtc cagcagcgtg tcgggcgcgc cgatggcgat atttgcctga
     2161 ttattaataa tcgcatccca gacgccgtta tacacttcgg tcgtcacggt aatctggcag
     2221 gtaggaaact gctttttcag cacctgtaat aagcgcgcgg tgtgtttggg cgtataaagc
     2281 agctgattaa tacaaatgcg cacacgcgct tctatgccct gcgaaatagt atcgatactg
     2341 cgtttaatgg cgtgaaaatc attcagcagg tcggtagctt tgcggaaaaa ataacgcccg
     2401 gactccgtta actcaatgct gcgggtactg cgagtgaaca gcaccacatc cagccccgtc
     2461 tccatacgtt taatggtgta gctaatggcg gaagtggtta aacccaactc ttctgcggct
     2521 ttactgaagc tgccaaaacg cgcagcggta gtaaatgcca acagattctc ttcggtaaaa
     2581 attgagttca ttaaaccatt cccgccatta tctaatatga atttctccag gcataactgt
     2641 agaggtatca attaaagatg ggtaatcatt tttgaataac ttttaatacc cgtcgcgttt
     2701 agtcgtcgct tcgcttaatt tttgtccgcc atattctgaa attatcccca cgcagttacc
     2761 cgccagaaaa cccagtaatg tcaccgtcca gttcatcccg ctggcaaaaa atagcgtcat
     2821 gccgagaaag cccccgggaa taaaagacag taaccagaaa cgcccttgcc agaccaccac
     2881 cgccgagaag ggtacagtcg taattacgct cgcccagacc ccggaaatcc cactcaccgg
     2941 ggccagccaa ccggcagcca gcgcggcgaa aaacgcccac accatcccgg aataattaac
     3001 cgccaggctt ctgataaacc ccgatttacc gccgcctgcg gcataaaaac tgcaaaaggc
     3061 gacgaagcca atggttccta ataattccca tccctgagag agcccgacat gtgaagacac
     3121 taactgccaa agccctgcac aaataccgac ggtgacaccc gttgccgtga gtccgttcat
     3181 cttgttcccc tctggttaga catggacgaa agctactctt acatgtgcag actatagaga
     3241 aataaaacct gattgagtga aagggtatcg ggtcaaagaa acaaatattg cactaccgca
     3301 cacggcgaaa agattgttga acatcattca acaaaaaggc gaaatacggc gaattaaatt
     3361 taagcgggtt cttttatcag attatcgttg ctattctcaa ttcatcgaaa acagccacaa
     3421 aaaccaggtg aatgtgaatg actataaaaa atctacctgc agactattta ttggctgcgc
     3481 aacagggtga tatcgataaa gtaaaaacct gccttgcgct gggtgtcgat attaatacct
     3541 gcgatcgtca ggggaaaacg gcaattacgc tggcaagttt atatcagcaa tatgcttgcg
     3601 ttcaggcatt aattgacgcc ggagcgaata ttaataaaca agatcatacc tgtttaaatc
     3661 cttttttaat gagctgtctg aacgatgatt taacgctact acgaattatt ttaccggcta
     3721 aacccgatct taattgcgta acccgttttg gcggtgtcgg cctgacgcct gcctgtgaaa
     3781 aaggccattt aagtattgta aaagagcttt tggcaaatac ggagattaac gttaaccaga
     3841 ctaaccatgt tggctggaca ccgttgctgg aagcgattgt gcttaacgat ggcgggatta
     3901 aacagcaggc gattgtccag ttattgctgg aacacggtgc cagcccgcat ctgaccgata
     3961 aatatggcaa aacgccgctg gaactggcgc gggaacgggg ctttgaagag attgcgcagt
     4021 tgctgatcgc cgcaggtgca taaaccggga ggcttgttat caacacacca gaaaaacggt
     4081 gtgtgtgggc gctaactgcg gatgctgatt ttctggcgca gcgggggcaa ggacaggttg
     4141 aacaggtctt tgccagagcg gtaaatatcg tactcccggc tcgccagcag ttgctgacgc
     4201 tgctttgtga agagtacgac aatgcgccta acagttgtcg gttggcactc actcacttta
     4261 atggtctgtt ccggcatggt gataaggttc agtttgacga tcaaggcatt acgattggtc
     4321 aacatcatca tatagagatg agtcattgcc agcgttggct gtccccaacc atgcaaatga
     4381 ccgctctgaa ttttcacctt atcgcctggc aacagtggca cgacattatt catcagcacc
     4441 tgggggaaaa tgaaaccctg tttaattatc gcggcgataa tccgttttat caggcgttaa
     4501 ataaagaact acatattaaa cgtcgggcag ttattcaggc cgtaaacgat aaacaaaata
     4561 tcgccgtagc ggtcgccagt atgatgggct tagggattgg ccttacgcca tcagccgacg
     4621 attatttaac aggtctggcg cttattttat ttcttcccgg acatccggcg gaaaaataca
     4681 aagaggaatt ttatctcggt ctgcaacgcg gcagaaataa taccacgtta ttaagtgcca
     4741 tcacgctgga agccgcatta caacaacgct gccgggaaaa tattcatcgt tttattcaca
     4801 acattattta tgacatccct gggaacgcaa ctcaggcaat agaaaaaatt aaacatattg
     4861 gctccagttc cggctgcgac atgctgtatg gcatggccga tggttgtgcg ctgagtcaaa
     4921 cttacggagg gaattatgtc agttaaaata gtcattaaac cgaataccta ttttgattct
     4981 gtctcgctga tgtctatctc cacgcgtgca aataaactcg acggcgtcga gcaggcattt
     5041 gtggcgatgg cgaccgaaat gaacaaaggc gtgttgaaga atttaggact gctgacgccg
     5101 gagctggagc aggcgaaaaa cggcgacctg atgattgtca tcaatggtaa atcgggtgtg
     5161 gacaacgagc agttgctggc ggagattgaa gaactgttca acaccaaagc gcaaagcggc
     5221 tcgcacgagg cgcgttacgc cactattgcc agcgccaaaa agcatatccc ggaaagtaac
     5281 ctggcagtga tttcggtcaa cggtctgttt gccgctcgcg aagcgcgtca ggcgctgcaa
     5341 aatgacctca acgtgatgct gttttccgat aatgtctcag ctgaagatga actggcgctc
     5401 aaacagttgg cccatgaaaa agggctgctg atgatggggc cagactgcgg cacggcgatt
     5461 atcaacggcg cggcactctg ctttggtaac gccgtgcgtc gcggcaacat cggtattgtt
     5521 ggcgcatctg gcaccggcag ccaggaattg agcgtccgta tccatgaatt tggtggcggc
     5581 gtttcacaac tgatcggcac cggcgggcgc gacctgagcg agaaaatcgg tggcctgatg
     5641 atgctcgatg ccatcgggat gctggaaaac gatccgcaaa ctgaaatcat tgtgcttatc
     5701 tccaaaccgc ctgcgcctgc ggtggctcgc aaagtgctgg aacgcgcacg cgcctgccgc
     5761 aagccagtgg tggcctgctt cctcggtcgt ggcgaaacgt cagtggatga gcaggggcta
     5821 cagtttgctc gcggcagcaa agaggcggca ttgaaagcgg tgatgctctc cggcgtgaaa
     5881 caagaacatc tcgacctgca tacgcttaac cagccgttga ttgcggatgt gcgggcgcgt
     5941 ctgcaaccgc agcagaaata cattcgtggt ctgttctgcg gcggtacgct gtgtgacgaa
     6001 accctgttcg cggtgatgga aaaacatggc gatgtctaca gcaatatcca gcccgatcct
     6061 gaattccgcc tgcaagatat caaccgcagc atcaaacaca ccttcctcga ttttggcgat
     6121 gatgacttca ccaacggcaa gccgcatcca atgattgacc cgaccaaccg tatcagccgc
     6181 ttgatcgaag aagcgcgcga tccagaagtg gcggtgatcg tgatggattt tgtgctcgga
     6241 tttggatcgc atgaagatcc ggtcggttcc accatcgagg cgatcaaaga agcgaaagcg
     6301 atcgccgctg ccgaaggacg cgagttgatc attctcgcct atgtgctggg caccgatctc
     6361 gatacgccat cattagaaca acaaagccag atgctgcttg atgccggagt gattctggcg
     6421 agcagcagca ccaataccgg attgctggcg cgtgaattta tctgcaaagg ggaggaagcc
     6481 tgatgagcca gtcactgttt agccaaccgt tgaacgttat taacgtcggt atcgccatgt
     6541 ttagcgatga tctgaaaaag cagcatgtag aagtgaccca actcgactgg acgccgcggg
     6601 gcagggcaat atgcaggtgg tgcaggcgct ggataacatt gccgattcgc cgctggcaga
     6661 caaaatcgcc gccgctaacc agcaggcgct ggagcgcatt atccagtcgc atccggtgct
     6721 gattggtttt gatcaggcga ttaatgtggt gccgggcatg acggcgaaaa ccattcttca
     6781 cgccgggccg ccggttacct gggaaaaaat gtgcggcgcg atgaaaggcg cggtcaccgg
     6841 ggcgctggtg ttcgaaggac tggcgaaaga tctcgacgag gcggctgaac tggcggcttc
     6901 cggggagatc accttctcgc cgtgccacga gcatgactgc gtgggatcga tggcgggcgt
     6961 cacctcggcc tcgatgttta tgcacatcgt gaaaaacaaa acctatggca acatcgctta
     7021 taccaacatg agcgagcaga tggcgaagat tttgcgtatg ggcgctaacg accagagcgt
     7081 gatcgaccgc ctcaactgga tgcgcgatgt gcagggaccc atgctgcgcg acgcgatgaa
     7141 aattatcggc gaaatcgatc tgcgcttaat gctggcgcag gcgctgcata tgggcgatga
     7201 atgccataac cgcaataacg ccgggacgac gctgctgatt caggcgctga cgccggggat
     7261 tattcaggca ggttattccg tcgagcaaca gcgcgaagtg tttgagtttg tcgccagcag
     7321 cgactacttc tccggcccga cgtggatggc gatgtgtaaa gcggcgatgg atgcggcgca
     7381 cggcatcgaa tacagcaccg tggtgaccac catggcgcgt aacggcgtcg agttcggcct
     7441 gcgggtcagc ggcctgccgg ggcagtggtt taccggcccg gcgcagcagg tcatcggccc
     7501 gatgtttgcc ggttataagc cggaagattc agggctggat attggcgaca gcgccatcac
     7561 cgaaacctac ggtattggcg gatttgcgat ggcgaccgcg cccgcgatcg tcgcgctggt
     7621 ggggggcacg gtggaagagg ctatcgattt ctcccgtcag atgcgtgaaa tcacccttgg
     7681 cgaaaacccc aacgtcacca ttccgctgct cggttttatg ggcgtgccgt cggcaatcga
     7741 catcacccgc gtgggtagca gcggcattct gccagtgatc aacaccgcca tcgcccataa
     7801 agatgcgggc gtcggcatga ttggcgcggg cattgtgcat ccacccttcg cctgcttcga
     7861 gaaagccatt tttggctggt gcgaacgtta cggcgtctga tctgtaaagt gcgcgttgtt
     7921 tctgccggat gcggcgtgaa cgccttatcc ggcctacgag tggcgcaaga atctgtaggc
     7981 ctgataagcg tagcgcatca ggcagtctgg cgttggtcat aaccccatca ccctctgtcg
     8041 cggacataac aacatgaaag aacttgtggt cgttgccatt ggtggcaaca gcattatcaa
     8101 agataacgcc agccagtcga ttgagcatca ggcggaggcg gtgaaagccg tggccgatac
     8161 ggtgctggaa atgctggctt ccgattacga cattgtgctg acccacggca acgggccgca
     8221 ggtcgggctg gatttacgcc gcgcggagat tgcccacgag cgcgaagggc tgcccttaac
     8281 gccgctggcg aactgtgtgg cggatacgca aggtggcatt ggctatctga tccagcaggc
     8341 gctgaacaac cgactggcgc gtcacggcga gaaaaaagcc gtcaccgtgg tgactcaggt
     8401 ggaagtggat aaaaacgatc cggggtttgc ccatcccacc aagcccatcg gcgaattctt
     8461 tagtgaaagc cagcgtgacg aactacaaaa ggcaaaccct gactggcgtt ttgttgaaga
     8521 tgccggacgg ggctatcgcc gcgtggtcgc ctcgccggaa ccgaaacgta ttgtcgaagc
     8581 acctgccatt aaggcgctga tccaacaagg ctttgtggtg attggcgcgg gcggcggtgg
     8641 aattcctgtg gtgcgcaccg aagcgggaga ttaccaaagc gtggacgcgg ttatcgacaa
     8701 agatctctct accgcgctac tggctcgcga aattcacgcc gacattctgg tgatcaccac
     8761 tggcgtcgaa aaagtgtgta ttcactttgg caaaccgcag cagcaggcgc tggatcgcgt
     8821 agatattgcc accatgaccc gctatatgca ggaagggcat ttcccacctg gcagcatgtt
     8881 gccaaaaatc atcgccagcc tgacgttcct ggaacaaggc ggcaaagaag tgattatcac
     8941 cacgccggaa tgcctgcctg cggcgctacg cggcgaaacg ggcacccata ttattaaaac
     9001 ctaaggacgt aagatgaaag aaaacaatag ccgccgtgaa tttctgagcc agagcggtaa
     9061 gatggttacc gccgccgcgc tgtttggtcc ctcagtgccg ctcgcccatg cggggggagc
     9121 tggcacccta aactgcgaag cgaacaacac catgaaaatc actgacccgc attactatct
     9181 cgataacgtg ctgctggaaa ccggttttga ctacgaaaat ggcgtggcgg tgcagacccg
     9241 cacggcgcgc cagactgtgg aaattcagaa cggtaaaatt gttgccctgc gcgagaacaa
     9301 gcagcatccg gacgccacgc tgccgcacta tgacgctggc ggtaagctga tgctgcccac
     9361 cacccgcgac atgcatattc atctcgacaa aaccttctac ggcgggccgt ggcgctcgct
     9421 caatcgtccg gcaggcacca ctatccagga catgatcaaa ctcgagcaga aaatgctgcc
     9481 ggaactgcaa ccgtacacgc aggaacgggc ggaaaaactg atcgatttat tgcagtcgaa
     9541 aggcaccacc attgcccgca gccactgcaa tatcgaaccg gtttccggcc tgaaaaatct
     9601 gcaaaatttg caggcggtgc tggcgcgacg tcaggcgggc ttcaagtgtg aaatcgtcgc
     9661 ctttccgcag cacggtttgc tgctgtcgaa atcggaatcc ttaatgcgcg aagcgatgca
     9721 ggcgggggcg cattacgtcg gcgggctgga cccgaccagt gttgatggcg cgatggaaaa
     9781 atccctcgac accatgttcc agattgcgct ggactatgac aaaggcgtcg atattcacct
     9841 gcacgaaacc acgccggcag gcgtggcagc catcaattat atggttgaaa cagtggagaa
     9901 aacgccacag ctgaagggca agctgaccat cagccacgcc tttgcgctgg caacgctcaa
     9961 cgaacaacag gtagatgaac tggcgcaccg gatggcggcg cagcgaattt cgatcgcttc
    10021 gacggtgccg attggcacgc tgcatatgcc gctcaaacag ctgcacgaca aaggcgtaaa
    10081 agtgatgacc ggcaccgaca gcgttatcga ccactggtcg ccttacggcc tgggcgacat
    10141 gctggaaaaa gccaatctgt acgcgcagct ttatattcgt cctaacgaac agaacctctc
    10201 ccgctcgctg tttttagcca ctggcgatat attgccgctg aatgaaaaag gcgagcgtgt
    10261 gtggcctaaa gcgcaggatg acgccagttt tgtgctggtg gacgcctcct gttccgccga
    10321 ggcggtggcg cgtatttcgc cgagaacggc gacgttccat aaagggcaac tggtgtgggg
    10381 aagtgtggca ggttgatacg catttgaaac gcctgcaacg gtgaatcgta agagatttaa
    10441 agccccaggg agtggggcgg cctgagtatg agtgtgttgg cagaaatgga cattatcttt
    10501 gaagaatatg cccttattga cgttgtgtag ataactattt gtctacgtga aaatatcatc
    10561 agttttgccg atttttgtct tttacggata gcaaatatca cacttatagg cgctcgcctt
    10621 atggggaacg ttgctgctct gtttcattca ggctcgcggc atgttggggc tggatcgggt
    10681 ggtttaaata ttggctatat tcaatggacg cgttttgccg cgatgacata tcaggcatcg
    10741 ccaaatacac atagctaatc aggagtaaac acaatgaaga tcaaagctgt tggtgcatat
    10801 tccgctaaac aaccgcttga accgatggat atcacccggc gtgaaccggg accgcatgat
    10861 gtcaaaatcg aaatcgctta ctgtggcgtc tgccattccg atctccacca ggtccgttcg
    10921 gagtgggcgg ggacggttta cccctgcgtg ccgggtcatg aaattgtggg gcgcgtggta
    10981 gccgttggtg atcaggtaga aaaacatgcg ccgggcgatc tggtcggtgt cggctgcatt
    11041 gtcgacagtt gtaaacattg cgaagagtgt gaagacgggc tggaaaacta ctgtgatcac
    11101 atgaccggca cctataactc gccgacaccg gatgaaccgg gccatactct gggcggctac
    11161 tcacaacaga tcgtcgttca tgagcgatat gttctgcgta ttcgtcaccc gcaagagcag
    11221 ttggcggcgg tggctccttt attgtgtgca ggaatcacca cgtactcgcc gctacgccac
    11281 tggcaggccg ggccgggtaa aaaagtgggc gtggtcggca tcggcggtct gggacatatg
    11341 gggattaaac tggcccacgc gatgggggcg catgtggtgg catttaccac ttctgagtca
    11401 aaacgcgaag cggcaaaagc cctgggggcc gatgaagttg ttaactcacg caatgccgat
    11461 gagatggtgg ctcatgtgaa gagtttcgat ttcattttga atacagtagc tgcgccacat
    11521 aatctcgacg attttaccac cttgctgaag cgcgatggca ccataacgct ggttggtgcg
    11581 cctgcgacac cgcataaatc gccggaagtt ttcaacctga tcatgaaacg ccgcgcgata
    11641 gctggttcta tgattggcgg cattccagaa actcaggaga tgctcgattt ttgcgccgaa
    11701 catggcatcg tggctgatat agagatgatt cgggccgatc gaattaatga agcctatgag
    11761 cgaatgctgc gtggtgatgt gaaatatcgt tttgttatcg ataatcgcac actaacagac
    11821 tgaaaaaatt aataaacatc ctgtggttta acatattaac ttcgctccac ttaacttttt
    11881 agttaaggag agcgaaataa tatcaaagta gtggtaaacc tatagcgtaa attcagattg
    11941 ttaaattaac tccactactt tacacgttag ttatgagcga tttctgatag tgtctggttt
    12001 aatcagagct ttattatctg cgacgtttat ttttatttaa gagagccacc tgcaagttgt
    12061 tattccacca gagagtgaat cgcaaagcag gtcatcattt catctgtata tctgtatgcc
    12121 aaatggcatt ccccctcctt ttccgccgat attttgttgt gaacggcgga gtttcgctta
    12181 aaaaagcacc agcagtggtt ttacagctat atggtgtata aaaaatgttc tcatacagat
    12241 gttttgtgaa tgtgtgggtt gacattcata tgaaaaaaat cataattcca tcatgtttgt
    12301 gtggggtctt ttctgtatct tacgcatcgc actcaagcct gacagaaaat atgttgtaag
    12361 gctcatatca aaaaccgcca ttagctcatc aggaagagca gacgacaacc aggattgttg
    12421 tatggtacgg ggttcgaggc ctcgatggcg gtacaacttt tcaaaacttt tctgaatcag
    12481 atccccttat tatcatccct gcaaagtgga acattgccat tgtttggcta ttcaggacgg
    12541 gtcagaccaa tgaaaaagcc cacacagggg agagtggctg aaatgggaag ctaaagactc
    12601 aagtaaactt atcggaaata aggaccacgc attacggggg ctatcatcgc cgatggttgg
    12661 gattcggcaa atcaggttga ttacgtagag agtaaattat ctgctcaccg ctgcgtcacc
    12721 ccttcgtaaa tcaggcgtag cgcgaataca ccaataattg caccaataac ccaactggca
    12781 acgcgttgca tacgcccata agcacgacgc actgcgggca aagaaaacgc ctgactaaga
    12841 aagactcgcc agataattga tgcgagcaca atccccgccc aggccattaa tcgtgcccat
    12901 gttggtgttt cggcatgtaa tgttactgag aaaatactga taaaaaataa aacggtttgc
    12961 gggttagaga gatcggtaat taatccacgg cggaaaaaaa cataccaggg ggcgctaatc
    13021 ggttgttgta gtgtgctcat ttgcggtgtt gagtggcggc gcatgctact acaccacgca
    13081 aaccataaga gataagcgcc gccgacgatt ctgataagcg aaaaaatctc cccacactgc
    13141 gtaattagcg ttgcaagacc aaacaaaccc aacccggaat aaaatgcatc gcccagcgcc
    13201 acgcccagcc cggtcaacac ccctgcgcgt cgaccggaag ccaggctggt ttgtactacc
    13261 acaaagagat tggctcccgg attaaaaaaa gtaatcacga acaatcctac ggtcaggtaa
    13321 acggcgtgca aaggatccat agtgatttca tccataaata agtgaagtaa cttcatcata
    13381 tttctaccaa aaataatcgg gtgcgagaga gatcacaaag tgtcttattt ccggttactg
    13441 gcgtttatgc cctgactgaa ctaattatta atcaacccaa taatgtgggt gggtgatagt
    13501 gtgataacaa ctctggagcc gtaatatgaa aataatctct aaaatgttag tcggtgcgtt
    13561 agcgtttgcc gttaccaatg tctatgccgc agaattaatg accaaagcgg aatttgaaaa
    13621 ggttgcatcg caatatgaaa aaataggtga tatttcaacc agtaatgaaa tgtcgaccgc
    13681 agatgcgaaa gaagatttga tcaaaaaagc cgatgaaaaa ggggctgatg tgttggtact
    13741 gacctctggt caaactgaca ataagatcca cggcacggca aatatttata agaagaagta
    13801 attctgaatc ctatgtaaac atccccgatg cgtaagttta tcggtgatca tcatctattg
    13861 aaatttatgc cggataaagc gttcgcgctg cattcggcag ttcagctttt cagccgccgc
    13921 cagaacgtcg ttcggctgat acctaaataa ttcgccgctg ctgtcttatc gccattaaat
    13981 ttctccagtg cctgttgtgc tgtcagcaag cctggaatgg gagtcttcgc cgactcgcgt
    14041 gccagttccg gcagtagcag ctgcaaaaat tgcggcgtta aatccggcgt cgattccaca
    14101 cttaaaaaca gcgccagtcg ttccatcata ttgcgcagtt cacgaatatt gcccggccag
    14161 tcgtagtgca ccagcatggt ttcgcttgcc tgtaacccct ggcgtaatgc ggcagcaaac
    14221 ggggcggaga gcgccgccag agacactttc aaaaagcttt ccgccagcgg cagaatatcc
    14281 gccacccgct cgcgtagcgg cggcagttgc aggcgtaaaa tactcagccg ataaaacagg
    14341 tcacggcgaa actgcccttg ctgcatatct tcttccagat tgcagtgagt ggcgctaatg
    14401 acccgcacat ccaccggaac aggctgatgc ccgccgacgc gggtgacctc tttttcttcc
    14461 agcacccgca gcagccgagt ctgcaacggc agcggcattt cgcctatctc gtcaagaaac
    14521 agcgtgccac cgtgggcgat ttcaaacagc ccggcgcgtc ctccgcgtcg cgagccggta
    14581 aacgcccctt cctcatagcc aaacagctcc gcttccagca gcgattcggc aatcgccccg
    14641 cagttaacgg caacaaacgg atgtgacttt ttgccctgtc gcgcatcgtg gcgggcaaaa
    14701 tattcccgat gaatcgcctg ggccgccagc tctttgcccg tccccgtttc cccttcaatc
    14761 aacaccgccg cactggagcg ggcatacagc aaaatagtct gccgcacctg ttccatctgt
    14821 ggtgattgac cgagcatatc gcccagcacg taacgagtac gcagggcgtt gcgggtggca
    14881 tcgtgagtgt tatggcgtaa cgacatgcgc gtcatatcca gcgcatcgct gaacgcctgg
    14941 cgtacggtgg cggcggaata aataaaaatt ccggtcattc cggcttcttc tgccagatcg
    15001 gtaatcagcc ccgcgccgac caccgcttcg gtgccgttag ctttcagctc gttaatctgc
    15061 ccgcgcgcgt cttcttcggt aatgtagcta cgttggtcga ggcgcaaatt aaaggttttt
    15121 tgaaacgcca ccagcgccgg aatagtttcc tgataagtga caacgccgat agaagaggtg
    15181 agttttccgg cttttgccag tgcctgtaac acatcgtagc cgctcggttt aatcaaaata
    15241 actggcactg acaggcggct tttcaggtac gcaccgttag agccagcggc aatgatggcg
    15301 tcacagcgtt cgcttgccag tttcttgcgg atgtaggtca ccgctttttc aaagccaagc
    15361 tggatagggg tgatattcgc caggtgatca aactcgaggc tgatatcgcg aaataactcg
    15421 aacagccgcg ttacagatac cgtccagata accggtttgt cgtcattcag ccgtggtgga
    15481 tgtgccatag cgcaccgcaa agttaagaaa ccgaatattg ggtttagtct tgtttcataa
    15541 ttgttgcaat gaaacgcagt gaaacattgc ttgaaacgtt aactgaaacg catatttgcg
    15601 gattacttca tgactttatt tataacaaat tgaaattaaa catttaattt tattcagtca
    15661 attgtggcac accccttgcc ttgtctttat caacgcaaat aacaagttga taacaaagga
    15721 tgggctatgc ctctacactc tccaggtaaa gcgtttcgcg ctgcacttag caaagaaacc
    15781 ccgttgcaaa ttgttggcac catcaacgct aaccatgcgc tgctggcgca gcgtgccgga
    15841 tatcaggcga tttatctctc cggcggtggc gtggcggcag gatcgctggg gctgcccgat
    15901 ctcggtattt ctactcttga tgatgtgctg actgacattc gccgtattac cgacgtttgt
    15961 tcgctgccgc tgctggtgga tgcggatatc ggttttggtt cttcagcctt taacgtggcg
    16021 cgtacggtga aatcaatgat taaagccggt gcggcaggat tgcatattga agatcaggtt
    16081 ggtgcgaaac gctgcggtca tcgtccgaat aaagcgatcg tctcgaaaga agagatggtg
    16141 gatcggatcc gcgcggcggt ggatgcgaaa accgatcctg attttgtgat catggcgcgc
    16201 accgatgcgc tggcggtaga ggggctggat gcggcaatcg aacgagcgga ggcctatgtt
    16261 gaagcgggtg ccgagatgct gttcccggaa gcaattaccg aactcgccat gtaccgccag
    16321 tttgccgatg cggtgcaggt gccgatcctt gccaacatta ctgaatttgg tgccacgccg
    16381 ctgtttacca ccgacgaatt acgcagcgcc catgtcgcaa tggcgctgta tcccctttct
    16441 gctttccgcg ccatgaaccg cgccgctgaa catgtctaca acgtcctgcg ccaggaaggc
    16501 acgcagaaaa gcgtcatcga caccatgcaa acccgcaacg agctgtacga aagcatcaac
    16561 tactaccagt acgaagagaa gctcgacgac ctgtttgccc gtggtcaggt gaaataaaaa
    16621 cgcccgttgg ttgtattcga caaccgatgc ctgatgcgcc gctgacgcga cttatcaggc
    16681 ctacgaggtg aactgaactg taggtcggat aagacgcata gcgtcgcatc cgacaacaat
    16741 ctcgacccta caaatgataa caatgacgag gacaatatga gcgacacaac gatcctgcaa
    16801 aacagtaccc atgtcattaa accgaaaaaa tcggtggcac tttccggcgt tccggcgggc
    16861 aatacggcac tctgcaccgt gggtaaaagc ggcaacgacc tgcattaccg tggctacgat
    16921 attcttgatc tggcggaaca ttgtgaattt gaagaagtgg cgcacctgct gatccacggc
    16981 aaactgccaa cccgtgacga actcgccgcc tacaaaacga aactgaaagc cctgcgtggt
    17041 ttaccggcta acgtgcgtac cgtgctggaa gccttaccgg cggcgtcaca cccgatggat
    17101 gttatgcgca ccggcgtttc cgcgctcggc tgcacgctgc cagaaaaaga ggggcacact
    17161 gtttctggtg cgcgggatat tgccgacaaa ctgctggcgt cacttagttc gattcttctc
    17221 tactggtatc actacagcca caacggcgaa cgcatccagc cggaaactga tgacgactct
    17281 atcggcggtc acttcctgca tctgctgcac ggcgaaaagc cgtcgcaaag ctgggaaaag
    17341 gcgatgcata tctcgctggt gctgtacgcc gaacacgagt ttaacgcttc cacctttacc
    17401 agccgggtga ttgcgggcac tggctctgat atgtattccg ccattattgg cgcgattggc
    17461 gcactgcgcg ggccgaaaca cggcggggcg aatgaagtgt cgctggagat ccagcaacgc
    17521 tacgaaacgc cggacgaagc cgaagctgat atccgtaagc gggtggaaaa caaagaagtg
    17581 gtcattggtt ttgggcatcc ggtttatacc atcgccgacc cgcgccacca ggtgatcaaa
    17641 cgcgtggcaa agcagctctc gcaggaaggc ggctcgctga agatgtacaa catcgccgat
    17701 cgcctggaaa cagtgatgtg ggagagcaaa aagatgttcc ccaatctcga ctggttctcc
    17761 gctgtttcct acaacatgat gggtgttccc accgagatgt tcacaccact gtttgttatc
    17821 gcccgcgtca ctggctgggc ggcgcacatt atcgaacaac gtcaggacaa caaaattatc
    17881 cgtccttccg ccaattatgt tggaccggaa gaccgccagt ttgtcgcgct ggataagcgc
    17941 cagtaaacct ctacgaataa caataaggaa acgtacccaa tgtcagctca aatcaacaac
    18001 atccgcccgg aatttgatcg tgaaatcgtt gatatcgtcg attacgtcat gaactacgaa
    18061 atcagctcca gagtggccta cgagaccgca cattactgcc tgctcgacac gctcggctgc
    18121 ggtcttgaag ctctcgaata cccggcctgt aaaaaactgc tggggccaat tgtccccggc
    18181 actgtcgtac ctaacggcgt gcgcgtcccc ggaactcagt tccagctcga ccccgtccag
    18241 gcggcattta acattggcgc gatgatccgt tggctcgatt tcaacgatac ttggctggcg
    18301 gcggagtggg ggcatccttc cgacaacctc ggcggcattc tggcaacggc agactggctt
    18361 tcgcgcaacg cggtcgccag cggcaaagcg ccgttgacca tgaaacaggt gctgaccgca
    18421 atgatcaaag cccatgaaat tcagggctgc atcgcgctgg aaaactcctt taaccgcgtc
    18481 ggcctcgacc acgttctgtt agtgaaagtg gcttccaccg ccgtggtcgc cgaaatgctc
    18541 ggcctgaccc gcgaggaaat tctcaacgcc gtttcgctgg catgggtaga cggacagtcg
    18601 ctgcgcacct atcgccatgc gccgaacacc ggtacgcgta aatcctgggc ggcgggcgat
    18661 gccacttccc gcgcggtacg tctggcactg atggcgaaaa ccggcgaaat gggttatccg
    18721 tcagccctga ctgcgcctgt gtggggcttc tacgacgtct cctttaaagg cgaatcgttc
    18781 cgcttccagc gcccgtatgg ttcttacgtg atggaaaatg tgctgttcaa aatctccttc
    18841 ccggcggagt tccactccca gacggcagtt gaagcagcga tgacgctcta tgaacagatg
    18901 caggcagcag gcaaaacggc ggcggatatc gaaaaagtga ccattcgcac ccacgaagcc
    18961 tgtattcgca tcatcgacaa aaaagggccg ctcaataact cggctgaccg cgaccactgc
    19021 attcagtaca tggtggcgat cccgctgctg ttcgggcgct taacggcggc agattacgag
    19081 gacaacgttg cgcaagataa acgcatcgac gccctgcgcg agaagatcaa ttgctttgaa
    19141 gatccggtgt ttaccgctga ctaccacgac ccggaaaaac gcgccatcgc caatgccata
    19201 acccttgagt tcaccgacgg cacgcgattt gaagaagtgg tggtggagta cccaattggt
    19261 catgctcgcc gccgtcagga tggcattccg aagctggtcg ataaattcaa aatcaatctc
    19321 gcgcgccagt tcccgactcg ccagcagcag cgcattctgg aggtttctct cgacagaact
    19381 cgcctggaac agatgccggt caatgagtat ctcgacctgt acgtcattta agtaaacggc
    19441 ggtaaggcgt aagttcaaca ggagagcatt atgtctttta gcgaatttta tcagcgttcg
    19501 attaacgaac cggagcagtt ctgggccgag caggcccggc gtattgactg gcagacgccc
    19561 tttacgcaaa cgctcgatca cagcaatccg ccgtttgccc gttggttttg tgaaggccga
    19621 accaacttgt gtcacaacgc tatcgaccgc tggctggaga aacagccaga ggcgctggcg
    19681 atgattgccg tctcttcgga aacagaagaa gagcgcacct ttacctttcg ccagctgcat
    19741 gacgaagtga atgcggtggc gtcaatgctg cgctcactgg gcgtgcagcg tggcgatcgg
    19801 gtgctggtgt atatgccgat gattgccgaa gcgcatatta ccctgctggc ctgcgcgcgc
    19861 attggcgcta ttcattcggt ggtgtttggg ggatttgcct cgcacagcgt ggcggcgcga
    19921 attgatgatg ctaaaccggt gctgattgtc tcggctgatg ccggagcgcg cggtggcaaa
    19981 atcattccct ataaaaaatt actcgacgat gcgataagtc aggcgcagca tcagccgcgc
    20041 cacgttttac tggtggatcg cgggctggcg aaaatggcgc gcgttagcgg gcgggatgtc
    20101 gatttcgcgt cgttgcgcca tcagcacatc ggcgcgcggg tgccggtggc atggctggaa
    20161 tccaacgaaa cctcctgcat tctctacacc tccggcacga ccggcaaacc taaaggtgtg
    20221 cagcgtgatg tcggcggata tgcggtggcg ctggcgacct cgatggacac catttttggc
    20281 ggcaaagcgg gcggcgtgtt cttttgtgct tcggatatcg gctgggtggt agggcattcg
    20341 tatatcgttt acgcgccgct gctggcgggg atggcgacta tcgtttacga aggattgccg
    20401 acctggccgg actgcggcgt gtggtggaaa attgtcgaga aatatcaggt tagccgcatg
    20461 ttttcagcgc cgaccgccat tcgcgtgctg aaaaaattcc ctaccgctga aattcgcaaa
    20521 cacgatcttt cgtcgctgga agtgctctat ctggctggag aaccgctgga cgagccgacc
    20581 gccagttggg tgagcaatac gctggatgtg ccggtcatcg acaactactg gcagaccgaa
    20641 tccggctggc cgattatggc gattgctcgc ggtctggatg acaggccgac gcgtctggga
    20701 agccccggcg tgccgatgta tggctataac gtgcagttgc tcaatgaagt caccggcgaa
    20761 ccgtgtggcg tcaatgagaa agggatgctg gtagtggagg ggccattgcc gccaggctgt
    20821 attcagacca tctggggcga cgacgaccgc tttgtgaaga cgtactggtc gctgttttcc
    20881 cgtccggtgt acgccacttt tgactggggc atccgcgatg ctgacggtta tcactttatt
    20941 ctcgggcgca ctgacgatgt gattaacgtt gccggacatc ggctgggtac gcgtgagatt
    21001 gaagagagta tctccagtca tccgggcgtt gccgaagtgg cggtggttgg ggtgaaagat
    21061 gcgctgaaag ggcaggtggc ggtggcgttt gtcattccga aagagagcga cagtctggaa
    21121 gaccgtgagg tggcgcactc gcaagagaag gcgattatgg cgctggtgga cagccagatt
    21181 ggcaactttg gccgcccggc gcacgtctgg tttgtctcgc aattgccaaa aacgcgatcc
    21241 ggaaaaatgc tgcgccgcac gatccaggcg atttgcgaag gacgcgatcc tggggatctg
    21301 acgaccattg atgatccggc gtcgttggat cagatccgcc aggcgatgga agagtaggtc
    21361 ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatgcg acgctgacgc
    21421 gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg ccgcatccga
    21481 caccatgcgc agatgcctga tgcgacgctg acgcgtctta tcatgcctac ccactgtttt
    21541 tacacagata agtttccccc gcctttttgt actcattcat ataagaaata tatcccccta
    21601 cgaaaacgat tgctttttat cttcagatga atagaatgcg gcggattttt tgggtttcaa
    21661 acagcaaaaa gggggaattt cgtgtcgcaa gataacaact ttagccaggg gccagtcccg
    21721 cagtcggcgc ggaaaggggt attggcattg acgttcgtca tgctgggatt aaccttcttt
    21781 tccgccagta tgtggaccgg cggcactctc ggaaccggtc ttagctatca tgatttcttc
    21841 ctcgcagttc tcatcggtaa tcttctcctc ggtatttaca cttcatttct tggttacatt
    21901 ggcgcaaaaa ccggcctgac cacccatctt cttgctcgct tctcgtttgg tgttaaaggc
    21961 tcatggttgc cttcactgct gctgggcggc acccaggttg gctggtttgg cgttggcgtg
    22021 gcgatgtttg ctattccggt gggcaaggca accgggctgg atattaattt gctgattgcc
    22081 gtctccggtt tactgatgac cgtcaccgtc ttctttggca tttcggcgct gacagttctt
    22141 tcggtgattg cggttccggc tatcgcctgc cttggcggtt attccgtgtg gttggccgtt
    22201 aacggcatgg gcggactgga cgcattaaaa gcggtcgttc ccgcacaacc gttagatttc
    22261 aatgtcgcgc tggcgctggt tgtggggtca ttcatcagtg cgggtacgct caccgctgac
    22321 tttgtccggt ttggtcgtaa tgccaaactg gcggtgctgg tggcgatggt ggcctttttc
    22381 ctcggcaact cgttgatgtt tattttcggt gcagcgggcg cggcggcact ggggatggcg
    22441 gatatctctg atgtgatgat tgctcagggc ctgctgctgc ctgcgattgt ggtgctgggg
    22501 ctgaatatct ggaccaccaa cgataacgcg ctctatgcgt cgggtttagg tttcgccaac
    22561 attaccggta tgtcgagcaa aaccctttcg gtgattaacg gtattatcgg tacggtctgc
    22621 gcattatggc tgtataacaa ttttgtcggc tggttgacct tcctttcggc agctattcct
    22681 ccggtgggcg gcgtgatcat cgctgattat ctgatgaacc gtcgccgcta tgagcacttt
    22741 gcgaccacgc gtatgatgag tgtcaattgg gtggcgattc tggctgtcgc gctggggatt
    22801 gccgcaggcc actggttacc gggaattgtt ccggtcaacg cggtattagg tggcgcgctg
    22861 agctatctga tccttaaccc gattttgaat cgtaaaacga cagcagcaat gacgcatgtg
    22921 gaggctaaca gtgtcgaata atgctttaca agcaattatt aacgcccggt tacccggcaa
    22981 agaggggctg tggcagattc atcttcacga cggaaaaatc agcgccattg atacgcaatc
    23041 tggtgtgatg cccgtaactg aaaacagcct ggatgccgaa caaggtttag ttttaccgcc
    23101 gtttgtggag ccgcatattc acctggacac cacgcaaacc gccggacaac cgaactggaa
    23161 tcagtccggc acgctgtttg aaggcattga acgctgggcc gagcgcaaag cgttattaac
    23221 tcatgacgat gtgaaacaac gcgcatggca aacgctgaaa tggcagattg ccaacggcat
    23281 tcagcatgtg cgtacccatg tcgatgtttc ggacgcaacg ctgactgcgc tgaaagcaat
    23341 gctggaagtg aaacaggaag ttgcgccgtg gattgatctg caaatcgtcg ccttcccgca
    23401 ggaagggatt ttgtcgtatc ccaacggtga agcgttgctg gaagaggcgc tacgcttagg
    23461 ggcagatgtt gttggggcga ttccgcattt tgaatttacc cgtgaatacg gcgtggagtc
    23521 gctgcataaa accttcgccc tggcgcaaaa atacgaccgt ctcatcgacg ttcactgtga
    23581 tgagatcgat gacgagcagt cgcgctttgt cgaaaccgtt gctgccctgg cgcaccgtga
    23641 aggcatgggc gcgcgagtca ccgccagcca tactacggca atgcactcct ataacggggc
    23701 gtatacttcg cgcctgttcc gtttgctgaa aatgtccggt attaactttg tcgccaaccc
    23761 gttggtcaat attcatctgc aaggacgttt cgatacgtat ccaaaacgtc gcggcatcac
    23821 gcgcgttaaa gagatgctgg agaccggcat taacgtctgc tttggtcacg atgatgtctt
    23881 cgatccgtgg tatccgctgg gaacggcgaa tatgctgcaa gtgctgcata tggggctgca
    23941 tgtttgccag ctgatgggct acgggcagat taacgacggc ctgaatttaa tcacccacca
    24001 cagcgccagg acgttgaatt tgcaggatta cggcattgcc gccggaaaca gcgccaacct
    24061 gattatcctg cctgctgaaa atgggtttga tgcgttgcgc cgtcaggttc cggtacgtta
    24121 ttcggtacgt ggcggtaagg tgattgccag cacacaaccg gcacaaacca ccgtatatct
    24181 ggagcagcca gaagccatcg attacaaacg gtaatcggct gggctaccgc gagcgtagtt
    24241 tatgccggat gcggcgtgaa caccttatcc ggcctacgta gatcactgaa attgtaggcc
    24301 tgataagcgg agcgcatcag gtaattttgc ttatttaaac tgaagactca actttaaaat
    24361 ctttgaaata atattgctta tcccggtcgt ttatttcgcg aataatccga caaggaacgc
    24421 cagcagccac gacgtttggt ggaatgtctt ttgtgacgac actacccgcg ccaataacag
    24481 aattatcccc gatggtgacg cctggattaa taaccacatg acttccgatc cagacgttat
    24541 tgccaatcgt tatcggaaaa gagtacattt ctccgttttt tctcaattca tggtgtaccg
    24601 ggtgtcccgt aacggaaaga gtaacgttgg gtgcaatcag tacgttatca ccgatcgtta
    24661 ccgtgtagtc gtcgacaatg gttaaattga aatttgcata aaaattgcgg cctatatgga
    24721 tgttggaacc gtaagagaaa tagacggggg ggtctaccca ggcgttttcc cctaccgtgg
    24781 caaacatttc tttaatcagg ctttctcttt tatcaacttc tgatggatgc gagtgattaa
    24841 actcatacat taacgttttt ccacgaagtc ttttttcagg taagccttcg cacatatcgg
    24901 taaatagctt gcctgctttt attctttctg tcatcgacat gttcattgcg atcattccgc
    24961 cctgatatgt tggtcggata aggcgctcgc gccgcatccg acattaattg cttaagcgac
    25021 ttcattcacc tggcgacgca gcaaggaaag cgggccgggg ccgctaagcg tgaacacgga
    25081 aattaaggtg aagcccagcg ccaccagacc cagcaccaga taagcgccct ggaaaccgat
    25141 gctttcatac atattgcccg ccagtacaga cataaaaatc atcgccagtt gcttaaagaa
    25201 gcagaaacag accagataaa tcgtcgctga aaaacgcact tcaaactggc tggtaatata
    25261 tttaaagcag cccaccagca ggaacggtac ttcaaacata tgcagcgttt tcagaataac
    25321 tacttccagc gctgaggtgg cgaacgatga gccaataata cgtacagaca taatagtgcc
    25381 agccagcagc agggcatttt tcccaccgat gcgattaatg atcagtggcg caaagaacat
    25441 aatcgaggca ttaagtaatt cgcccattgt cgttacgtag ccaaataccc gcgtaccctg
    25501 ttcaacggta gcaaaaaacg aagtaaagaa attagcaaac tgttggtcaa aaacatcgta
    25561 ggtgcaggaa acgccaataa catacagtga caaaaaccac agttttggct gtctgaacag
    25621 ttccagcgcc agcttaaggc taaatgccga atggttggca cctaccgcat tggcaaccgt
    25681 ggctgaagag ggcgcatccg ttttggcgaa aaagagtaaa acggcgagga tgagtgcaca
    25741 gccagaaccc agccagaaaa caaactgatt attgatggtg aacatgatgc cgacaatcga
    25801 ggcacacagc gcccagccaa cacagccaaa catccgcgcg cgaccaaatt cgaaattact
    25861 gcgacggctg actttctcga taaatgcctc tactgctggc gcaccggcgt taaaacaaaa
    25921 gccaagataa ataccaccaa caatcgatcc tactaaaatg ttgtattgta acagtggtcc
    25981 gaagataaaa ataaagaacg gcgcaaacat cactaacatg ccggtaataa tccacagcag
    26041 gtatttgcgc agcccgagtt tgtcagaaag cagaccaaac agcggttgga ataatagcga
    26101 gaacagagaa atagcggcaa aaataatacc cgtatcactt ttgctgatat ggttgatgtc
    26161 atgtagccaa atcgggaaaa acgggaagta ggcccccatg ataaaaaagt aaaagaaaaa
    26221 gaataaaccg aacatccaaa agtttgtgtt ttttaaatag tacataatgg atttccttac
    26281 gcgaaatacg ggcagacata gcctgcccgg ttattattat ttttgacacc agaccaactg
    26341 gtaatggtag cgaccggcgc tcagctggaa ttccgccgat actgacgggc tccaggagtc
    26401 gtcgccacca atccccatat ggaaaccgtc gatattcagc catgtgcctt cttccgcgtg
    26461 cagcagatgg cgatggctgg tttccatcag ttgttgttgg ctgtagcggc tgatgttgaa
    26521 ctggaagtcg ccgcgccact ggtgtgggcc ataattcaat tcgcgcgtcc cgcagcgcag
    26581 accgttttcg ctcgggaaga cgtacggggt atacatgtct gacaatggca gatcccagcg
    26641 gtcaaaacag gctgcagtaa ggcggtcggg atagttttct tgcggcccca ggccgagcca
    26701 gtttacccgc tctgcgacct gcgccagctg gcaggtcagg ccaatccgcg ccggatgcgg
    26761 tgtatcgctt gccaccgcaa catccacatt gatgaccatc tcaccgtgcc catcaatccg
    26821 gtaggttttc cggctgataa ataaggtttt cccctgatgc tgccacgcgt gggcggtcgt
    26881 aatcagcacc acatcggcaa gtgtatctgc cgtgcactgc aacaacgccg cttcggactg
    26941 gtaatggccc gccgccttcc agcgttcgac ccaggcgtta gggtcaatgc gggtcgcttc
    27001 acttacgcca atgtcgttat ccagcggcgc acgggtgaac tgatcgcgca gcggggtcag
    27061 cagttgtttt tcatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg
    27121 ccaacgctta ttacccagct cgatgcaaaa atccgttccg ctggtggtca gttgagggat
    27181 ggcgtgggac gcggagggga gtgtcacgct gaggttttcc gccagacgcc attgctgcca
    27241 ggcgctgatg tgtccggctt ctgaccatgc ggtcgcgttt ggttgcacta cgcgtaccgt
    27301 tagccagagt tgtccggcgc tctccggctg cggcagttct ggcagttcaa tcaactgctt
    27361 accttgcggg ccaacatcca gaggcacttc accgcttgcc agcggcttgc catccagcgc
    27421 caccatccag tgcaggaact cgttatcgct atgacggaac aggtattcgc tggtcacttc
    27481 gatggttcgc ccggataaac ggaactgaaa atactgctgt tggtgttttg cttccgtcag
    27541 cgccggatgc ggcgtgcggt cggcaaagac cagaccgttc atacagaact ggcgatcgtt
    27601 cggcgtatcg ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt catcatattt
    27661 aatcagcgac tgatccaccc agtcccagac gaagccgccc tgtaaacggg ggtactgacg
    27721 aaacgcctgc cagtatttag cgaagccgcc aagactgtta cccatcgcgt gggcatattc
    27781 gcaaaggatc agcgggcgca tttctccagg cagcgaaagc cattttttga tggaccattt
    27841 cggcaccgcc gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc
    27901 ggttgcggag gtgtcggctc cgccgccttc atactgtacc gggcgggaag gatcgacaga
    27961 tttgatccag cgatagagtg cgtcgtgatt agcgccgtgg cctgactcat tccccagcga
    28021 ccagatgatc acactcgggt gattacgatc gcgctgcacc atccgcgtta cgcgttcgct
    28081 catcgcgggt agccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt
    28141 ttcaatattg gcttcatcca ccacatacag gccgtagcgg tcgcacagcg tgtaccacag
    28201 cggatggttc ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcatcagcag
    28261 gatatcctgc accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg
    28321 acggttaacg ccgcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat
    28381 ccgcacctcg cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt
    28441 gtgcagttca accaccgcac gatagagatt cgggatttcg gcgctccaca gttccggatt
    28501 ttcaacgttc aggcgtagcg tgacgcgatc ggcataaccg ccacgctcat cgataatttc
    28561 accgccgaaa ggcgcggtgc cgctggcgac ctgcgtttca ccctgccaca aagaaaccgt
    28621 cacccgcagt tcatcgcgca gctcgccgta catctgaact tctgcctcca gtactgcgcg
    28681 gctgaaatca tcattaaagc gagtggtaac ttggaaatcg ctgatttgcg tggtcggttt
    28741 atgcagcaac gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag
    28801 ataactgccg tcactccagc gcagcaccat caccgcgagg cggttttctc cggcgtgtaa
    28861 aaatgcgctc aggtcaaatt cagacggcaa acggctgtcc tggccgtaac cgacccagcg
    28921 cccgttgcac cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc
    28981 ttcctgtagc cagctttcat caatattaaa tgtgagcgag taacaacctg tcggattctc
    29041 cgcgggaaca aacggcggat tgaccgtaat gggataggtc acgttggtgt agataggcgc
    29101 atcgtaaccg tgcatctgcc agtttgaggg gacgacgaca gtatcggcgt caggaagatc
    29161 gcgctccagc cagctttccg gcaccgcttc tggtgccgga aaccaggcaa agcgccattc
    29221 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    29281 ccagctggcg aaagggggat gtgccgcaag gcgattaagt tgggtaacgc cagggttttc
    29341 ccagtcacga cgttgtaata cgacggccag agaatccgta atcatagtca tagctgtatc
    29401 ctgtgtgaaa ttgttatccg ctcacaattt cacacaacat acgagccgga cacatagagt
    29461 gtaaagactg gggtgcctaa tgagtgagct gactcacatt aattgtgttg cgctcactgc
    29521 ccgctttcca gtcgggaaac ttgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    29581 ggagaggcgg tttgcgtatt gggcggaagg gtggtttttc ttttcaccag tgagacgggc
    29641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
    29701 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacacgag
    29761 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
    29821 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
    29881 ggaacgatgc cctcatttag catttgcatg gtttgttgaa aaccggacat ggcactccag
    29941 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagccc
    30001 gccaggcgca gacgtgccga gacagaactt aatggacctg ctaacagcgc gatttgctgg
    30061 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
    30121 atactgttga tgggagtctg gtcagagaca tcaagaaata acgccggaac attagcgcag
    30181 gctgcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagccaactg
    30241 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
    30301 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
    30361 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
    30421 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
    30481 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
    30541 gtctgataag agacaccggc atactccgcg acatcgtata gcgttactgg tttcacattc
    30601 accaccctga attgactctc ttccgggcgc tatcatgcca taccacgaaa ggttttgcgc
    30661 cattcggcgg ggctgacgcc gaagtgagat ttaaaatgct gacgccaggt cacaagtgag
    30721 agaaaaccca cgccaataca cctacggcgt ttacaggtat actcgctaaa aattattcag
    30781 cgggtttgga aacaaagatg gcaaaactta ccttacaaga gcagttgctc aaagcgggac
    30841 tagtcaccag caaaaaagcg gcgaaggtcg agagaacggc gaaaaaatcg cgtgttcagg
    30901 cgcgtgaagc tcgggcggcg gtagaagaaa ataaaaaggc acagcttgag cgtgataaac
    30961 agcttagcga gcagcaaaaa caagcggcgt tggcgaaaga atataaagcc caggtgaagc
    31021 agctcattga aatgaaccga attaccattg ccaatggcga tattggtttt aacttcactg
    31081 acggcaatct gattaagaaa atctttgtcg ataagctcac gcaaacgcag ttgattaatg
    31141 gtcgtctggc gattgcccgc ttgttggtcg ataacaataa cgaaggtgaa tacgccatta
    31201 ttcccgccag cgttgccgat aaaattgctc agcgtgatgc cagcagtatt gtgttgcaca
    31261 gcgcgctcag cgccgaagag caggatgaag acgatccgta tgccgacttc aaagtgcctg
    31321 atgatttgat gtggtaaaca tagataagcc gtgcagtgat gcctgatgcg acgcttacgc
    31381 gtcttatcag gcctacggtt tacgggcgaa gtgtaggccg gataaggcgt tcacgccgca
    31441 tccggcagcc gtgcgcagat gcctgatgcg acgcttttcg cgtcttatca ggcctacggt
    31501 ttacgagcga aatgtgggcc ggataaggcg tttacgccgc atccggcagt cgtgcactat
    31561 tatcaacgca tattcagttt attggcgtgg tacgcaatat gctcgccaat aaaactggag
    31621 acaaaataat agctgtgatc ataaccctcg tgataacgga ttaacgtctt gatattcatc
    31681 tcctggcaga tcttttcaag atttggagtc cgcagctgtt ctgcgtaaaa atcatcactc
    31741 aatccctgat caaccatgat ttccgcaacg cgttgacctt gtgaaataag actcaccggg
    31801 tcgtaatcca accaggcatc tttattttca ccaagatatg cggcaaaggc ttgctgtccc
    31861 cacggtactt gcgatgggga gacaatgggc gaaaacgccg agacgctggc atattcatct
    31921 gggttacgta acgccagcac cagcgcgccc agcccgccca tagaatgacc tgagatagac
    31981 tttctggccg ttgctggaaa atgatgcatc actaaattcg gcagctcgtt gcggatatag
    32041 tcatacattt tgtaatgttc attccacggc gcttgcgtcg cgttcaggta aaacccggct
    32101 ccttgcccga gatcgtaacg gtcagcatct gcgacatgac tgcctcgcgg actggtgtcc
    32161 ggcgcaacaa caataatgtt gtgctcagcc gcgtaacgct gcatccccga tttagtaatg
    32221 aaattctgct cgttgcaggt caggcctgaa agccagtaca acaccggcaa tttttcattc
    32281 tcggcttttg gtgggagata gacgccgaca ttcatttcac atttcagtga ttgggaataa
    32341 tgccgataca cattttgcca gccgccaaag ctggcatgtt tttcaatgag ttccatcagt
    32401 cattatctca cggttaatcc cccggacggc tgcctgggga gagtattaag aaccccgtct
    32461 gaactggacg gggtaaacca gcgggaaatc agtaacgaat tacggttcga atggatttac
    32521 cttcatgcat caggtcgaag gcgtcattaa tttcatccag gctcatggta tgcgtgacaa
    32581 acggttccag atcgatatca cctttcatcg catcttcaac catacccggt aactgggaac
    32641 gacctttcac gccgccaaac gcggaacctt tccatacgcg accggtgacc aactggaatg
    32701 gacgggtgga gatttcctga ccggagcctg ctaccccgat gatcaccgac tgaccccagc
    32761 cgcggtgcgc gctttccagc gctgcacgca tcacgttgac attaccgatg cattcaaagg
    32821 tatggtcgat accccatttg ttgatatcca gcagaacatc tttaattggt ttgtcgtagt
    32881 cattcgggtt aatgcagtcg gtggcaccga agcgacgcgc cagttcgaat ttcttcgggt
    32941 tggtatcgat agcgataatc cgtcccgctt tcgcctgacg cgcgccctga accactgcca
    33001 gaccaatcgc gccaagacca aacacggcaa cagaatcacc tggctggact ttagctgtgt
    33061 tgtgtaccgc gccaataccg gtggtcacgc cacagcccag caggcagacg tgttcatggt
    33121 tcgcttctgg attaattttg gccagagaca cttccgctac cacggtgtat tcactgaatg
    33181 ttgagcatcc catgtagtga taaagcggct gcccgttgta agaaaaacgg gtggtgccgt
    33241 ctggcatcaa gcctttgccc tgcgtttcgc gaaccgcgac acacaggtta gttttgccag
    33301 aacgacagaa ctcacactcg ccgcactctg cggtgtaaag cgggatcaca tggtcgccag
    33361 gtttgacgct ggttacccct tcaccgactt caactacaac cccggcccct tcgtgaccga
    33421 gaaccaccgg gaagacacct tccgggtcat cgccggagag ggtaaatgcg tcggtatggc
    33481 aaacaccggt atgggtgact ttaatcagca cttcaccttt tttcggtggt gcaacgtcaa
    33541 tttcaacgat ttccagcggt ttaccgggag caaatgcaac ggcagcacgt gatttcatct
    33601 ctcgctcttc ctcaatatgg taatagattc agctatttaa gataggcacg aaccagttca
    33661 atagtgtcgt caacggattg gctgacttcg cggctgtagc agtcatttcg gtcaaacgtt
    33721 tcccggatat ggctttcaag cacttctgcc atcagcccgt tagccgcgcc ccgaacggca
    33781 gcgatctgtt ggagtatggc acggcattcg gcatcaccct ccagcgaccg ttccagagca
    33841 tcaatctgcc cccgaatacg acgaactcga gtaaggacct ttttcttctc ttccggagta
    33901 ctgggcattt cgcacctcat catctgcacg caatatacta taggggggta tgctatatgt
    33961 caatgcatac ccccctatag tatataacac tggagaataa aatttatccg gtgaatgtag
    34021 tcggaaaaca aagaggaaag ggggtagggg ctaatcggca gggaaggccg ccccggatag
    34081 cgggcggcag aaggaatcag aatttccagg tcagatgggc tgcaagttgc agaccgttat
    34141 aatcatcggt tggggtgtcg taccacactt tacctgccgt cagcccgaga ttaagttgct
    34201 cagtcagcgg ttgaatacgt tgtaaactga cgctcttcat gctgccgtag cgtgtttctg
    34261 gcgtccagtc ataggtgtaa gcgcctgttc cgcggcttag ccatagttgc gtataaccag
    34321 tgccgcgcgg gttattcaga cgcacggaaa tcatattgct gtaactacca cctgcatcgc
    34381 tggagtcata atgggtatag cggtagctgg agatcaccgg gccagtatag agtgatatgc
    34441 cgccttgcca ggcatcgact tcgacatcat cgtaatattt agtgtagcga taaccggtcg
    34501 tgaaaagcgt ttttggcaac agggccaggt taatatcctg acgaaaatcc tggcgggcaa
    34561 agaccggcgt attatccgca aaggcaatac ccgttcgggt tgtcagccag ttattccatt
    34621 tataccagac cgcaccctga ccgcgagtgg cattgaaatg ttcagtttca taatcacgac
    34681 gcccctggga aagattaaaa agcagcgttg tgttttcaac tttcgccacc agttcagcat
    34741 acgctaaatt acggttgcca tgatcgccag aataatcggt aaaatcgtag cccgcggtaa
    34801 tggaagttaa cccggcataa acgggtaatg cgctaaaaat tgccgccgct aataacgtcc
    34861 gttttatcat tttaatctcc tgtacggata agttcttgtc ggagtgaata agttccggta
    34921 ggtaaattac gttctacaat cacgcgcggg ccaagttgcg tgttcgggga aataattcgc
    34981 ccaggcaaaa taataacttg tacgccaagg cgtgaacgct gcccgatata acaacctaat
    35041 ttatcgcatc cggtagcgat aattccctct ggagttcgaa cagacacggg ctgttcatcc
    35101 agacgatgat tactggttcg tacttgtgcg cccaaatatg cctggtttgc aactaccgag
    35161 tcggcaataa agcattgcgg accaatcgtt gcttccgctt caataaccgc atttttaatt
    35221 tcggtggcaa aaccaat
//
LOCUS       DAZFRE010000049        34758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFRE010000049 DAZFRE010000000
VERSION     DAZFRE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.049"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            44..973
                     /gene="pfkB"
                     /locus_tag="ACHKUI_002685"
     CDS             44..973
                     /gene="pfkB"
                     /locus_tag="ACHKUI_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6598856.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLAHENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1074..1364
                     /gene="ghoS"
                     /locus_tag="ACHKUI_002686"
     CDS             1074..1364
                     /gene="ghoS"
                     /locus_tag="ACHKUI_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6598857.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            1470..2330
                     /gene="yniA"
                     /locus_tag="ACHKUI_002687"
     CDS             1470..2330
                     /gene="yniA"
                     /locus_tag="ACHKUI_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6598858.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2371..2907)
                     /gene="yniB"
                     /locus_tag="ACHKUI_002688"
     CDS             complement(2371..2907)
                     /gene="yniB"
                     /locus_tag="ACHKUI_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6598859.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3054..3722
                     /gene="hxpB"
                     /locus_tag="ACHKUI_002689"
     CDS             3054..3722
                     /gene="hxpB"
                     /locus_tag="ACHKUI_002689"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6598860.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLIEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKAHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3886..4476
                     /gene="ydjM"
                     /locus_tag="ACHKUI_002690"
     CDS             3886..4476
                     /gene="ydjM"
                     /locus_tag="ACHKUI_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6598861.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4609..6000
                     /gene="tcyP"
                     /locus_tag="ACHKUI_002691"
     CDS             4609..6000
                     /gene="tcyP"
                     /locus_tag="ACHKUI_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6598862.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESITSFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6047..6310)
                     /gene="cedA"
                     /locus_tag="ACHKUI_002692"
     CDS             complement(6047..6310)
                     /gene="cedA"
                     /locus_tag="ACHKUI_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6598863.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6493..8754
                     /gene="katE"
                     /locus_tag="ACHKUI_002693"
     CDS             6493..8754
                     /gene="katE"
                     /locus_tag="ACHKUI_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6598864.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCSYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVSADDGTVLSIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADCASGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(8801..9559)
                     /gene="chbG"
                     /locus_tag="ACHKUI_002694"
     CDS             complement(8801..9559)
                     /gene="chbG"
                     /locus_tag="ACHKUI_002694"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6598865.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFVAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(9572..10924)
                     /gene="celF"
                     /locus_tag="ACHKUI_002695"
                     /gene_synonym="chbF"
     CDS             complement(9572..10924)
                     /gene="celF"
                     /locus_tag="ACHKUI_002695"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6598866.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11028..11870)
                     /gene="chbR"
                     /locus_tag="ACHKUI_002696"
     CDS             complement(11028..11870)
                     /gene="chbR"
                     /locus_tag="ACHKUI_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6598867.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(11878..12228)
                     /gene="chbA"
                     /locus_tag="ACHKUI_002697"
     CDS             complement(11878..12228)
                     /gene="chbA"
                     /locus_tag="ACHKUI_002697"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6598868.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12279..13637)
                     /gene="chbC"
                     /locus_tag="ACHKUI_002698"
     CDS             complement(12279..13637)
                     /gene="chbC"
                     /locus_tag="ACHKUI_002698"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO6598869.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13722..14042)
                     /gene="chbB"
                     /locus_tag="ACHKUI_002699"
     CDS             complement(13722..14042)
                     /gene="chbB"
                     /locus_tag="ACHKUI_002699"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6598870.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14341..14679)
                     /gene="osmE"
                     /locus_tag="ACHKUI_002700"
     CDS             complement(14341..14679)
                     /gene="osmE"
                     /locus_tag="ACHKUI_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6598871.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            14881..15708
                     /gene="nadE"
                     /locus_tag="ACHKUI_002701"
     CDS             14881..15708
                     /gene="nadE"
                     /locus_tag="ACHKUI_002701"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6598872.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            15938..16825
                     /gene="cho"
                     /locus_tag="ACHKUI_002702"
     CDS             15938..16825
                     /gene="cho"
                     /locus_tag="ACHKUI_002702"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6598873.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWNGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNNQQAS"
     gene            complement(16785..17360)
                     /gene="ves"
                     /locus_tag="ACHKUI_002703"
     CDS             complement(16785..17360)
                     /gene="ves"
                     /locus_tag="ACHKUI_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6598874.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGYSPDQVQ"
     gene            complement(17563..18048)
                     /gene="spy"
                     /locus_tag="ACHKUI_002704"
     CDS             complement(17563..18048)
                     /gene="spy"
                     /locus_tag="ACHKUI_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6598875.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18378..19346)
                     /gene="astE"
                     /locus_tag="ACHKUI_002705"
     CDS             complement(18378..19346)
                     /gene="astE"
                     /locus_tag="ACHKUI_002705"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6598876.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19339..20682)
                     /gene="astB"
                     /locus_tag="ACHKUI_002706"
     CDS             complement(19339..20682)
                     /gene="astB"
                     /locus_tag="ACHKUI_002706"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6598877.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFRGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQIFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSAYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(20679..22157)
                     /gene="astD"
                     /locus_tag="ACHKUI_002707"
     CDS             complement(20679..22157)
                     /gene="astD"
                     /locus_tag="ACHKUI_002707"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6598878.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRATGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAGYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22154..23188)
                     /gene="astA"
                     /locus_tag="ACHKUI_002708"
     CDS             complement(22154..23188)
                     /gene="astA"
                     /locus_tag="ACHKUI_002708"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6598879.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEMAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGEFPACLVANENYHHFRVVLVRTDPATGRLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23185..24405)
                     /gene="astC"
                     /locus_tag="ACHKUI_002709"
     CDS             complement(23185..24405)
                     /gene="astC"
                     /locus_tag="ACHKUI_002709"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6598880.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYACMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGSNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            24851..25657
                     /gene="xthA"
                     /locus_tag="ACHKUI_002710"
     CDS             24851..25657
                     /gene="xthA"
                     /locus_tag="ACHKUI_002710"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6598881.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            25824..26534
                     /gene="ydjX"
                     /locus_tag="ACHKUI_002711"
     CDS             25824..26534
                     /gene="ydjX"
                     /locus_tag="ACHKUI_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6598882.1"
                     /translation="MNAERKFLLACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            26539..27216
                     /gene="ydjY"
                     /locus_tag="ACHKUI_002712"
     CDS             26539..27216
                     /gene="ydjY"
                     /locus_tag="ACHKUI_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6598883.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            27230..27937
                     /gene="ydjZ"
                     /locus_tag="ACHKUI_002713"
     CDS             27230..27937
                     /gene="ydjZ"
                     /locus_tag="ACHKUI_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6598884.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            27937..28485
                     /gene="ynjA"
                     /locus_tag="ACHKUI_002714"
     CDS             27937..28485
                     /gene="ynjA"
                     /locus_tag="ACHKUI_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6598885.1"
                     /translation="MGLPPLSKIPFILRPQTWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            28495..29661
                     /gene="ynjB"
                     /locus_tag="ACHKUI_002715"
     CDS             28495..29661
                     /gene="ynjB"
                     /locus_tag="ACHKUI_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6598886.1"
                     /translation="MRLCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            29634..31169
                     /gene="ynjC"
                     /locus_tag="ACHKUI_002716"
     CDS             29634..31169
                     /gene="ynjC"
                     /locus_tag="ACHKUI_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6598887.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALQLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            31169..31822
                     /gene="ynjD"
                     /locus_tag="ACHKUI_002717"
     CDS             31169..31822
                     /gene="ynjD"
                     /locus_tag="ACHKUI_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6598888.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGKNLLLALP
                     ATLNGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVTLRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            31889..33196
                     /gene="ynjE"
                     /locus_tag="ACHKUI_002718"
     CDS             31889..33196
                     /gene="ynjE"
                     /locus_tag="ACHKUI_002718"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6598889.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFIYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(33205..33825)
                     /gene="ynjF"
                     /locus_tag="ACHKUI_002719"
     CDS             complement(33205..33825)
                     /gene="ynjF"
                     /locus_tag="ACHKUI_002719"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6598890.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            33912..34319
                     /gene="nudG"
                     /locus_tag="ACHKUI_002720"
     CDS             33912..34319
                     /gene="nudG"
                     /locus_tag="ACHKUI_002720"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6598891.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(34285..34557)
                     /gene="ynjH"
                     /locus_tag="ACHKUI_002721"
     CDS             complement(34285..34557)
                     /gene="ynjH"
                     /locus_tag="ACHKUI_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6598892.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
BASE COUNT         8554 a         9098 c         8585 g         8521 t
ORIGIN      
        1 gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac gtatctatac
       61 gttgacactt gcgccctctc ttgatagcgc aacaattacc ccgcaaattt atcccgaagg
      121 aaaactgcgc tgtaccgcac cggtgttcga acccggtggc ggcggcatca atgtcgcccg
      181 cgccattgcc catcttggag gcactgccac agcgatcttc ccggcgggtg gcgcgaccgg
      241 cgaacatctg gtttcactgt tggcgcatga aaatgtcccc gtcgctactg tagaagccaa
      301 agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgagc agtatcgttt
      361 tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagctcgaag agcaagttct
      421 ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag gtgtgaagct
      481 ggaaaaatta acccaactga tttccgctgc gcaaaaacaa gggatccgct gcatcgtcga
      541 cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt tggttaagcc
      601 taaccaaaaa gaactcagtg cgctggtaaa tcgcgaactc acccagccag acgatgtccg
      661 caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg tttccctggg
      721 tccacaaggg gcgctgggcg ttgatagtga aaactgcatt caggtggtgc caccaccggt
      781 gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga cacttaaact
      841 ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg ggagtgcagc
      901 cacactcaac cagggaacac gcctgtgctc ccatgacgat acgcaaaaaa tttacgcgta
      961 cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca acctgtcggc
     1021 aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga actatggcca
     1081 gtggcgatct tgtccgttat gtaataaccg tcatgttgca tgaggataca ttgactgaaa
     1141 ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg acggatgatg
     1201 atggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc caaagtgaag
     1261 aagaaattag agaactggtt tcggggctta cccacagtgc aaccggcaaa gatcctgaaa
     1321 tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt tttgggcaat
     1381 aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg ctaaccttat
     1441 gatctggcag acaacatggg agagacatca tgtggcaggc aattagtcgt cttttgagcg
     1501 agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga gaagtccatg
     1561 ccgcatggca tttgcgctat gcaggacgtg actttttcgt caaatgtgat gaaagggaac
     1621 tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt agtaaaaccg
     1681 tcaccgtgcc taaggtttgg gcagttggcg ctgaccgtga ctacagtttt ctggtgatgg
     1741 attatctccc acctcgcccg ctggatgcgc atagtgcatt tattcttggt cagcaaattg
     1801 cgcgtttaca tcaatggagc gaccaaccac aatttggcct cgatttcgat aacgcgctct
     1861 ccacaactcc acagcccaac acctggcaac gtcgctggtc aacttttttt gctgaacaac
     1921 ggattggctg gcagctggaa ctggcggcag aaaaagggat cgctttcggc aatatcgatg
     1981 ccatcgtcga gcatattcag cagcgtctcg cctcgcatca accgcagcct tctctgttgc
     2041 acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac attttcgacc
     2101 cggcctgtta ctggggtgac cgagagtgcg acctggcgat gttaccgctg catactgaac
     2161 agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc gatttccttg
     2221 aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaaggtta tttggcggtc
     2281 agcatttggt tattgctcag cagtcactgg atagattatt agcagcatga tatgggttga
     2341 ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag aaaagaacac
     2401 ataacctgca gcaatacaaa taaccggcag gatatacagc gagaaaaact gcaggaaaat
     2461 agtatggtgc ggaacaacaa tacgtgactc aatttgttca cgcgttagcc cctcttcccc
     2521 tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg
     2581 actcatccgg gctccggaat cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac
     2641 ccagaacata acgttcacgc caccattaaa attgggcgtc ggagagttgt accagaacag
     2701 attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagtaa agtcgagcat
     2761 gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca gcaaagaaat
     2821 caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt tcaaaacagc
     2881 aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg tcgtttcact
     2941 tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga taggcgctta
     3001 atcataagca cggcttaata ccttacatat aatgctctaa aggagaggtt gtaatgtcaa
     3061 ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc gactcagaac
     3121 ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtggat atctcccgtc
     3181 gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat ctttggtacg
     3241 cccggcaacc atggaatggg ccaagccgtc aggaagtagt agaacaagtt attgcccgtg
     3301 ccatttcact gattgaagag acacgtccat tattgccagg tgtgcgtgaa gccgttgcgt
     3361 tatgcaaaga acaaggttta ttggtggggc tggcctccgc gtcaccgctg catatgctgg
     3421 aaaaagtatt gaccatgttt gatttacgcg acagtttcga tgccctcgcc tcggctgaaa
     3481 aattgcctta cagcaaggcg catccgcaag tatatctcga ctgcgcagca aaactggggg
     3541 ttgaccctct gacctgcgtg gcactggaag attcggtaaa tggcatgatc gcctctaaag
     3601 cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg
     3661 tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac cttctcggtt
     3721 aatgaccagg gggcagtgat cacactgccc ctggttcttt atctgaattt gcattcaact
     3781 gacggattaa tcgccaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta
     3841 taaaaaccct atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt
     3901 caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg
     3961 ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg
     4021 gacatcgatc acccaaagtc gtttcttggg cagcgattaa agtggatatc aaaaccgatc
     4081 gcccgcgctt ttgggcatcg tggttttacc cacagtctac tggcggtatt tgcgctgctg
     4141 gcaacctttt accttaaagt tcctgaaagc tggttcattc cggctgatgc gttgcagggt
     4201 atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc
     4261 ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac
     4321 caactggaac gttttatctg catggcatta tttgtctggt cggtatggat gccccattca
     4381 ttacccgaaa acagcgctgt tcgttggtca tcgcaaatga tcaatacctt acagatccag
     4441 tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatcg cctttctgga
     4501 ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca
     4561 ctatgactgt tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca
     4621 ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca aacccgccac
     4681 aaacagtgga gtctggcgaa aaaagtgctg gtgggtcttg tgatgggtgt ggtttttggc
     4741 cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg
     4801 tttaacattg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgctagtc
     4861 ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc
     4921 agttttctga ccatcggtac gcttttgttt accacgctga ttgcagcgct ggtcggtgtg
     4981 ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact
     5041 gcacgtctga acgccatcga aagtaactat gttggtaaag tctctgacct gagcgttccg
     5101 cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atctgaccgg agccaatccg
     5161 acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc gctgaaactg
     5221 ctgaaggatg atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac cctgcaaagc
     5281 tgggtgatga aactggttcg cctggtcatg cagttgaccc cttacggtgt tctggcgctg
     5341 atgaccaaag tggttgcagg ttctaacctg caagacatca tcaaactggg aagtttcgtt
     5401 gttgcgtcct acctcggtct gctgattatg tttgcagtgc atggccttct gctgggcatt
     5461 aatggcgtga gcccgctgaa gtacttccgt aaggtatggc cagtactgac gtttgccttt
     5521 accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg
     5581 ggtgttcctg aatccatcac cagtttcgcc gcctctttcg gtgcgaccat tggtcagaac
     5641 ggttgtgccg gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac ggtgggcatt
     5701 aacccactgg acccgatgtg gattgcgacg ctggtcggta ttgtcaccgt tagttccgca
     5761 ggcgttgccg gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg
     5821 ggcctgccgg taacgctggt ggcattgtta atctccgttg aaccgcttat cgacatgggt
     5881 cgtacggcgc taaacgttag tggctcgatg accgctggca cgctgaccag ccagtggctg
     5941 aagcaaaccg ataaagccat tctggatagc gaagacgacg ccgaactggc acaccgttaa
     6001 tcctgatttg ttacgttaaa cgcctgtcat cccgacaggt gtttttttat tcttcgacct
     6061 cattttcctg acgaatctga tttgcccaac gctgggcgga ttcaggcaca gtaaacgccg
     6121 gtgagcgcag aaatgactcc cccatcagta caaacgcaac atatttgcca cgcagcatcc
     6181 agacatctcg aaacgaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg
     6241 ggacatagct aataatctgg cggttttgct gacggagcgg tttcttcatt actggcttca
     6301 ctaaacgcat attaaaaatc agaaaaactg tagtttagcc gatttagccc ctgtacgtcc
     6361 cgctttgcgt gtatttcata acaccgtttc cagaatagtt tccgaagcgg gatctggctg
     6421 gtggtctata gttagagggt tttttgacca aaacagcggc cctttcagta ataatttaag
     6481 gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta
     6541 cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgaaga cggatctcat
     6601 cgtccagcgg ctgaaccaac accgcctggt gcacaaccta ccgccccagg gagcctgaaa
     6661 gcccctgata cgcgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa
     6721 aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg acgatcaaaa ctcactgcgt
     6781 gccggtagcc gtggtccaac tctgctggaa gattttattc tgcgcgagaa aatcactcac
     6841 tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt
     6901 tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatacg
     6961 aacaaaatca ccccagtatt tgttaggttc tctaccgttc agggcggcgc tggctctgcc
     7021 gataccgtac gtgatatccg tggctttgcc accaagttct atactgaaga gggtattttt
     7081 gacctcgttg gcaataacac gccaatcttc tttatccagg atgcacataa attccccgat
     7141 tttgttcatg cggtaaaacc agaaccgcac tgggcaattc cacaagggca aagcgcccac
     7201 gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggca
     7261 atgtcggatc gcggtatccc gcgcagttac cgcactatgg aaggcttcgg tattcatacc
     7321 ttccgcctga ttaatgccga agggaaggca acgttcgtac gtttccactg gaaaccactg
     7381 gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaat taaccggacg tgacccggac
     7441 ttccaccgcc gcgagttgtg ggaagcgatt gaagcaggcg attttcctga atacgaactg
     7501 ggcttccagt tgattcctga agaagacgaa ttcaagttcg acttcgatct tctcgatcca
     7561 accaaactta tcccggaaga attggtgccc gttcagcgtg tcggcaaaat ggtgctcaat
     7621 cgtaatccgg ataacttctt tgctgaaaac gaacaggtgg ctttccatcc agggcatatt
     7681 gtccccggtc tggatttcac caacgacccg ttattgcagg ggcgtttgtt ctcttatacc
     7741 gatacacaaa tcagtcgtct tggtgggccg aatttccatg aaattccgat taaccgcccg
     7801 acctgctctt accataattt ccagcgtgac ggcatgcatc gtatggggat cgacactaac
     7861 ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccgggg
     7921 ccgaaacgcg gcggttttga atcataccag gaacgcgtgg aaggcaataa agttcgcgag
     7981 cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg
     8041 ccattcgaac agcgccatat tgtcgatggt ttcagttttg agttaagcaa agtagttcgt
     8101 ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag
     8161 gcggtggcga aaaatctcgg tatcgaactg actgatgacc agctgaatat caccccacct
     8221 ccggacgtca acggtctgaa aaaggatcca tccttaagtc tgtacgccat tcctgacggt
     8281 gatgtgaaag gtcgcgtggt agcaattttg cttaatgatg aagtgagatc ggcagacctt
     8341 ctggccattc tcaaggcact gaaggccaaa ggcgttcatg ccaaactgct ctactcccga
     8401 atgggggaag tgagtgcgga tgacggtacg gtgttgtcta tagctgctac ctttgccggt
     8461 gcgccttcgc tgacggtcga tgcagtcatt gtcccttgcg gcaatattgc ggatatcgct
     8521 aacaacggcg atgccaacta ctatctgatg gaagcctaca aacaccttaa accgattgcg
     8581 ctggcaggag acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca gggtgaagaa
     8641 gggattgcgg aagctgactg cgcctctggt agttttatgg atgaactgtt aacgctgatg
     8701 acagcacacc gcgtgtggtc acgcattcct aagattgaca aaatcccggc gtaaatatgg
     8761 ataaggtcac gccccctggg gagtgacctt accaccttac ttacacatca tgataactac
     8821 ccaaccgata accacgctca gcaatcgcat atttcaacga cgctgaagtc agcacatgca
     8881 gttccgttaa ccgagggaag caataagcac tctggcgaat cgtattatcg acaaacgccg
     8941 gatggcacat aacttccaac gagcgttccc cacgatggct ggaatcatca agcacttgca
     9001 ggaacaatgc ctcgctaatc tcttcaccat aaaacgcgct actgaatccc tgcgaactgc
     9061 gcagattagc cggtaaatca ccatcgttag atagcggttg acggtcgatg cgcaacgcaa
     9121 tcccctcttc agccacaaac tttgccacaa tcgggaaaat ctgcgggaac atatgcacat
     9181 gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact
     9241 gactggcgag ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc
     9301 agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agaggcttgc
     9361 ccatagtaag gacaaagtgc atccctatgg ccagactcgg ttcatcacgg ctcaactgca
     9421 ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgacgtgaca atcccattgc
     9481 gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca tcggcattaa
     9541 caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc
     9601 agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct
     9661 cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaag acatcgttaa
     9721 attctccact aagtgcggcg ttgctggcag caatctcgaa gcctttgatg gtgtgaatca
     9781 gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc
     9841 ccagcgtaca ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat
     9901 gcgggatatt aacgtaatgt tctgcttgct tgtcgttata gatagcgttg atcacttcgc
     9961 acgcagcatc agaataataa gccccaccgc gctgttccag ttctttcggc ttaacgttca
    10021 actccgggtt tttatatagc tcaaaaagtt gtttctcgac tttctgtact acctgcgctc
    10081 gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttt tgcttgaagt
    10141 aataaagcaa ataagaacac ggcaacagat tcatagaacg aattaagcct tcgctaaatg
    10201 gcagatcgaa aatattttta acgccagatg cttttaactg cccggatgcc acaccatcaa
    10261 gcaattcggc aaagcgtgat ttgccattta ccagcacatc cttaatgaac accatatggt
    10321 tgaggccgaa cagatcgata gataaatcat cgctgtcttt cagcatcaga acatcgcgaa
    10381 taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat
    10441 gacgataaac ggcttcagtg accattcccg ccgggttagt gaagttaatc acccatgcat
    10501 tcggacatag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac
    10561 ctttaaacag accgcccgcg ccgttagttt cctgaccaag ataaccatga cttaatggaa
    10621 tacgttcatc cagttcccgc gccggtaatt ggccaacgcg cagttgggta gtaacgaaat
    10681 cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag
    10741 cgttatcaat catccgttgg cagagatcaa aaataatatc cagtttcgct ttaccacctt
    10801 cgacatccac cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt
    10861 ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc
    10921 tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc cccggacata
    10981 aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat
    11041 tcagtcaatt tcttacgata gctcttaggc gtaaaggatg ttaatttctt aaacgtttta
    11101 ataaacaagc tcgggctact ataaccggcc tcaaacgcaa tatccgtcac tgaatagttg
    11161 gtcatttcca gttgtttttt ggcaaaatta atacggattt cattaataat ctgcattggc
    11221 gttttaccat agtatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac
    11281 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
    11341 gtacttttca gccactgcgg tatatcatca atcacctgtt cttcgcggta atgacgtaaa
    11401 cggttaatga cataaaaagt aaccatctca acaaactctt caagccctgt ttcgcggaaa
    11461 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
    11521 tgcgaagcta caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta
    11581 ctgatcccaa cgttcaatat acgcgtggca ccaaattcat aaaaactttg atggtgcgaa
    11641 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
    11701 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagtccg
    11761 ctgatactct cagttttatt gtagataaac acatggaagt ttttgccatt aaacaactgc
    11821 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
    11881 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
    11941 cgtcattaaa tgatcctgag cgtggactag caccagactc actttcatct taccttcgcc
    12001 agcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
    12061 ctgatccatc atcgcttttg ctgcggcaaa atcgccctgc ttcgcctgtt tcagtgccgc
    12121 atacgccagg ctacgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
    12181 ctcttcagct tccgtttgcg tatcgggaat attatcgaga tccatcatac atcgttcctc
    12241 tctattctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg
    12301 atatcttctt cgctctcttc tttatcaatc gcgttttgtg ctttgttagc gaccacaaca
    12361 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcgaccag caatgcggcg
    12421 acgctaccgt tggtgttaaa gaaggctccc agaccggtgg gcatggtcca cggtgcaata
    12481 ttggtcaccg gtggaataat gcccatgtag tacgctgcca gggtgattgc cgccagaatc
    12541 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
    12601 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
    12661 taatcagcac ggcgagaagc gataaagata gccaggatca ggcctaaagt cgcaccactg
    12721 ccaccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
    12781 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
    12841 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
    12901 aaccagagca gtggaacaaa gatcacatag gcccagccta ccacgctacc caacgatgcc
    12961 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
    13021 gcccaggcaa taatccccat cacggaaaga ataataaaac cgggaattaa tgctgagaaa
    13081 gagcgcgaaa ccgatgtcgg aacgctatca ggtaacttaa tgacccagtt acgatgaaca
    13141 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc
    13201 ccacctaacc agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc
    13261 ataaatgctg caacggataa caacccggcc gccagcgcat cgactttacg ctcttctgcc
    13321 agcgccatgc caataaagaa cggggccatt aaagacatta tccctaatgt tccgttatat
    13381 acgttgccgc caataccttt cagaccatta agtgtttcaa tggttgaggc atccaggcga
    13441 atacctaagg aataaaaaaa cgacccctcc ccaaagctta gaaaaacgtt gttaattaat
    13501 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
    13561 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
    13621 gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata ttgcggtatt
    13681 gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc tgcggctttt
    13741 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
    13801 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
    13861 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
    13921 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
    13981 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
    14041 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
    14101 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
    14161 acggtatagg cctgagcaat agtatttctt tgaccttata aattgtctct ccttgacctg
    14221 tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaacacagg
    14281 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca ggcgaagtga
    14341 ttacttcgta gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
    14401 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
    14461 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
    14521 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
    14581 ctgtacaaac tggtctttgg tacgatcata agccgtacaa cccgccagca tggttaatac
    14641 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
    14701 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgtgaa cgaaacggct
    14761 cctctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
    14821 gtttcagtag caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
    14881 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
    14941 gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta tccgttcatt
    15001 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg taagctgtgc
    15061 cagatggcga ttaatgagct gcgccaggaa accggcaacg aatcactgca atttattgcc
    15121 gtacgcctgc cctatggtgt tcaggccgac gaacaagact gccaggatgc catcgccttt
    15181 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtactcgc cagcgagcag
    15241 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
    15301 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
    15361 gatcatgcag cagaagctat taccggattc ttcactaaat atggtgacgg cggtacggac
    15421 attaatccgc tgtatcgtct caacaaacgt cagggtaaac agttactggc ggcattaggt
    15481 tgcccggaac acctttataa gaaagcgcca acggccgatc ttgaagatga tcgcccttca
    15541 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
    15601 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
    15661 aaacgccgtc cgccaattac cgttttcgat gatttctgga agaagtaata atttgcacat
    15721 attggtttgt gcgaaaaaga gtactttgtt cacgccggat gcggcgtgaa cgccttattc
    15781 gacctacaaa actatgcaaa ttcaatacat tgcaggagtc gaataggcct gacaggcgta
    15841 gcacgtcaga cggtgtaacc tttgtcatca acccgcctct tttttaatcg cttcccggct
    15901 gttatactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
    15961 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
    16021 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
    16081 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
    16141 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgca ccgcaggcga
    16201 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
    16261 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
    16321 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
    16381 taggcgcgca gctttgcaag cattgcagag catcgctgat gaacaaaaac tttgttatgg
    16441 cctgctggga ctggaaccgt taagccgcgg gcgtgcttgt tttcgttctg cattaaaacg
    16501 ttgcgctggg gcgtgttgcg gtaaagagag ccatgaggaa catgcgctac gtttgcgcca
    16561 gtctctggaa cgtttgcgag tcgtttgttg gccgtggaat ggtgcggtgg cgctgaaaga
    16621 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
    16681 taattcgctg gaagaagcga caacgttaat tcgggcaccc gccgggtttg atcacgacgg
    16741 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
    16801 ggtgaataac cagcaagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
    16861 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcagttta
    16921 tcgcccaatt gccacgcccc gttaatgaca aacactaccc cgccgcgtga gccaaaggta
    16981 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccagtcg tgtcatgatg
    17041 ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt ttaccaccag gtccgctgca
    17101 aaggaaaaag gttgtaacgg ttttaaggta tggttaaagc ggtctgcgct ttcaaggaac
    17161 atctcgccgc cttccagcaa cgtcactatc ctttccatac cgggaaacaa agaaaactcc
    17221 ccattcgccg cgatggatgt aatgctggca cgccagtaaa aatcacgttt tgccggtggg
    17281 aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc acagattcac cgacatttta
    17341 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
    17401 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggcgatc aggctttttt
    17461 tgcatctgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
    17521 tagtggacaa gaccggcggt cttacgtttt ttggctgaaa gattattcag cagttgcagg
    17581 cattttacct tttgccgctg gacgttctgt cagacgcttc tcaaaattag cattaaattg
    17641 ctttttctgt tccggcgtca ggatgttgta aatcttgttc tgggtttcca tgtgcgccag
    17701 catgttagct ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt ttgctttatc
    17761 gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt
    17821 catctggtca cgctggcctt tcatgatttc gcggatctgc tgtttctgcg cgtcggtcag
    17881 gttcaggtct ttgaacatca tgtcctcatg cggaccgaac ttgcctttat ggtgcatcat
    17941 cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc
    18001 gccaagagcc agggtagagg caacaaacag tgcagttaat ttacgcatat tctatatcct
    18061 tcctttcagt tatttattac ggctttctta agtagcgtgc cgtgttgacg agattaactt
    18121 tactgacttt agcgtcaatt aatcagagca acggtaaaac aatgaaagtg taaaaaacac
    18181 ttttgcgcca attatggaga aaaaaagaaa atttgatgga gagtgatgaa agaatattac
    18241 aacacgatga ttttgcagag attatgaaga actataccgg atgactggtg ataaataaag
    18301 caaataacca ggattaatct gtattaattt ataagaaagc aacttaatac ccgcagaatg
    18361 atttctgcgg gtaagtatta gcttattttt tcgagcatta atcccgcacg taatcccaac
    18421 gctaccaacg gattagggaa taacacatac tctacatcat gggttacggt aaaacgttcc
    18481 tccccgtcct gcgccagcaa tgttcctttc tcaaacggca taaaattcag cgtgtcattt
    18541 gccatatgca tttcgaagga cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg
    18601 agcggcggtg ttctcacgat accgacactc tcaccagata gcagcgcagc aattgcgctg
    18661 gcagttactg caaactggcg aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc
    18721 agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc
    18781 tcctgatgga acaccagcgc ctccagcccc gccgcaccca gccacgtcag aaatttctcg
    18841 tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg gatgcaagga gccacgaatt
    18901 gcggtatgta gatcaaggtg ccagcgcaca gattctttgc cctggtcata aaaatcttcc
    18961 aggcactgtt ccagttcgcg cgcccgacag gtttccccgc tttcagcaaa cagttgccaa
    19021 cgaccgccaa acattcgatt catatcgcta tggcaataac gtttcccttg cttcagcgca
    19081 ggaggattcc cgaggatcac cagtaaccgc caacgtaacg ggatctcacc gtgagatatc
    19141 gcgccaagca acgcgtccag catttccaca ggtgccgtct cattaccgtg gatccccgct
    19201 gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca gttccagcac accatcgccc
    19261 agccagcgcc agcgaacgcc gttgatttct cgctcggtta taaccggttt tttacccgtt
    19321 aaggttagag caagaaaatt atccattgcc ccctccctcg cgctggaatg gataaaccga
    19381 accaagatcc aataattgag tcagtgtatc cagcgcttcc cttccttcgc gcagcaattg
    19441 tggatcggca agatccgccg cagtaagacg atcgcggtag taacgatcca cccagtcatt
    19501 gagcgcatta aacagcgtat cgttcatcat caccgccgga ttcaccgccc ggcgttcttc
    19561 ttctgtcaac accacgcgca accgcaggca cgccgggcca ccgccattcg ccatgctttc
    19621 acgtaaatca aaaactttta gtgagcttat tgggttgtca gcagcaagca attcattgag
    19681 ataacgccat actccagcat gttcccgaca ttcctgaggc agcaccaaca tcatggaacc
    19741 atcatcgcgg ctcagcagtt ggctgttaaa cagataagca gacaccgcat ctgacacgga
    19801 aacctgcgcc gtgggcacct ctattgccat aaaaccactg acccgtgcac gcagatttgc
    19861 cagtaactgt gactggcgag cgaacgcctg ttgatggcaa aacagcacct ggcggttact
    19921 cacggcaatc acatcattat gaaaaacgcc ctggtcgata acgtcggggt tttgctgggc
    19981 gaaaatcacc tgctgtggat tcacctgatt cagccttgcc accgcctcgc tggcttcgcg
    20041 agtctgccgc gccggatagc gggacggtcg ggtatcattg ccctcctctc gcccgtaaac
    20101 aaaaatctgc attcccggtt caccgtaatg accgccgaga cgattatgat tcgcggctcc
    20161 ttcgtcaccg agcaaagcca cctgcggcaa cgccgaatgg acgctaaatt tctcttcgtc
    20221 gttaaaaatc gcttttaaca gcgattcggt gacgggggct tccagcgaac ggtgaaattt
    20281 attgttcagg ttagcaacag tgagatgcac tttgccatcc agcgtatcgg cggatggcgc
    20341 gatcgttgcc gcattggcta cccacattgg cgaagcggaa ctgacgctgg aaagccagtg
    20401 cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg ctacccctga atcccaactg
    20461 acgcagcacc ggaataaacg gacgctcgtg cggcgggatc acggcctggg ggaatcccgc
    20521 atcggcaagt tttttcattt tcagtaagcc ctgttttgcc gccagtcgcg ggttagacac
    20581 ctgaaaacgg tgacgggtag aggcttcatt accaaacgac aggcccgcgt aatggtgcgt
    20641 cagccctacc agcccgtcaa aattgacttc ccaggcgttc atcgcaccac ctcatcggaa
    20701 aaatccagcc cggggttaag cgttgcgggc aacgttaacg agtccgactc caggctcgcc
    20761 atcggccagg cacaataatc tgcggcatac ccggcgctgg ggcgatgatt gccggaagcg
    20821 ccaatgccgc cgaatggcgc ggtactggca gcaccggtaa gcggtttgtt ccagttaaca
    20881 atccccgccc gtgcctccag caacagttga tcgaattttt cccgctcggg ggaaaccaga
    20941 ccgcaagaga ggccgaagcg agtgttattc gccattcgaa tcgcttcatc gaaattgtca
    21001 taacgccaga cgcgcaataa cggcccaaac acttcttcat ccggcacgcc agtaacgcct
    21061 gtcatttcaa tgatccccgg cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca
    21121 agcagggtcc gtccgcccat cgcctccagt tcctgccagg cagtaaccac ctgctgtgcg
    21181 gcctgctcag aaatcagccc accaataaat ggctgcggtt cgtcatccca agtgcccggc
    21241 gttaaccgct ggctgacggc aaccaggcga gcaagaaatg catcgccctg cgttccactt
    21301 ttcagaaata aacggcgggc gcaggtgcag cgttgcccgg cagtgacaaa cgccgactga
    21361 atggtcagat ggacagcggc atcgatatcc gccacctcat cgataattag cgggttatta
    21421 ccgcccatct caagggcgag aattttctcc ggctgaccgg aaagctggcg atgcagctgg
    21481 tagccggtat tggcgctgcc ggtaaacagc aagccgtcga gatcctccag cgcactcagc
    21541 gcctgacccg ttgcacgccc gccctgcacc aggttcagca cgcccggcgg caagccagcc
    21601 tgctgccata aacgcattac cgcgtcgcca ctccacggtg tcagttcgct gggtttaaag
    21661 ataatggtgt tacctgccag caatgccgga acgatatgtc cgttcggcaa atgaccaggg
    21721 aaattatacg gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgcg
    21781 ccgtccggca tttcgctacg ctgctcaccg gtacgaacgt gatacgcctt aattgatatc
    21841 gcgattttat tgatcatcgc cgtcacttcg gttgccgctt cccagcgcgg cttaccggtt
    21901 tctctggcaa taatcgcggt taattcggct ttattgcttt ccagcaatcc ggcaaagcgt
    21961 tcaactctta cctgacgatc gccaaaggag agacgcgccc agcgcggaaa cgccgcacgg
    22021 gcagcccgac aagcctgccc gacctgagcg gcatcggcat cattgccctg ccataacacc
    22081 tcgcccgata ccggattacg cttcacacgc gatgcgccct ggcccgtaac ccagtcaccg
    22141 ttgatccata aagtcatgct gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat
    22201 cccctgcgtg gcatttgaga gcatccagtt gtgcagcggt taaaatcaaa cgcccggtcg
    22261 ctggatcggt acgcaccagc accacgcgga aatggtgata attttcattg gcgaccaggc
    22321 aggccgggaa ttcgccctgc gcaggctgcc cttcagccac ttccaccagc cgacttttac
    22381 ggatggcgcg cacgcggtcg atatcacatt caagtgtcgg cccgccgtca aagatgtcga
    22441 tatagttacg gtagcgaaaa ccttctttct ccagcaccgc acgggcaggc gcggtttgcg
    22501 gatgtacctg accgatgacg tcctgggctt cctgggataa aaagtgggta tagatcggat
    22561 gtttcggcat cagttctgca ataaatgcct tttgcccggt gccgcagaga aaatcagcgc
    22621 ggctaaaatc catcgaaaag aagcgtttac cgaggctttg ccagaacggt gaatagccgt
    22681 gttcgtcaat caccccgcgc atttcggcga ccactttgtc attaaacttg tcgcgaaaag
    22741 ccgccataaa cataaagcgc gatttcgaca gcaaatagcc gttgccctct ttgcgccact
    22801 ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca
    22861 gcgtcggcaa tgcgttatag acattcagct ctttcgaagc gtgaaccagc gtgccgacac
    22921 gatagttgta ccagggatcg ttcagcccaa ccgccatctc aatggcgcaa atccccgcca
    22981 cagtgcctgt ctcactatct tccagtacaa acacataacc ctgctcactt ttgggcagtt
    23041 cgccttgcca ggttttgatt gccctttcaa tacgcaccga aagcgtggct tcattggcgg
    23101 gaagcgacgt caggccgccg cccgttttgc tggcaagctg catcagcgcc gcaacgtctg
    23161 agcgttcaac gggacgaatg accatcatga tgaacctcgg ctaacaaagt gttcgcaagc
    23221 agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc
    23281 aaagcgcacc acgttgctcc ccgcaatcag caccatcacg cctgctttca ccgcttcctg
    23341 agagatctgt ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa tcaaaagacc
    23401 taagccgcga acttcactga acaaactgta gtggtgatta atggaattta gacgctcaac
    23461 aaaccagtcg tgacgctgtt taaccccgtt aagcatctct ggcgtgttga tgagatccag
    23521 cactttgcct gccaccgccg aggccagcgg attgccgcca taggtggtgc cgtgagtgcc
    23581 aacggtcatc acgctggcgc actcttcggt tgccaacaac gcaccaaccg ggaaaccgcc
    23641 gcccagcgct ttggcggtag ttaacagatc aggcgtcacg ccgtagtgca tacaggcata
    23701 cagttccccg gtgcgcccga caccggtttg tacttcatca aagatcagca acgcattgtg
    23761 gcggtcacac aattcacgca gaccttgtaa aaatgcgttg ctggctggca ccacaccgcc
    23821 ttccccctgg atgggttcga caatcaccgc acaggtggcg tcgtcaatca gcgcactggc
    23881 agagttaata tcgttatatg cagcatgacg aatatccggc ggcagtggcg caaaatcctg
    23941 tgaataggct ggctgcccac ccgcactgac agtaaacagc gtgcgaccat gaaacgcatt
    24001 tttgaacgcc acgatgccgc tcttatggct gccgtagcgg tcgtgagcga atttacgcgc
    24061 cagttttagt gccgcttcgt tggcttccgc accggagtta caaaagaaga cgcgatcggc
    24121 aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc ggctcgttgg tgtaaccgtt
    24181 gccggtatgc cagaacttac tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc
    24241 atggcccagc gcgttcaccg caatgccacc cgcgaagtcg atatactctt tcccctgctg
    24301 atcccacaag cgtgaacctt cgccacgtac cggtataaac ggtgccggag cgtaaacagg
    24361 tatcatccat tcatcaaagt tttcacgcgt aattggctga gacatagcga cctctacagt
    24421 aaataattcg ttatttatat gttaataata agtaatgttt gcgctgtaaa tgtagattgc
    24481 agggttcgtg ccagccagtg ataaaagtgc ataaacggcg gaggctaact ggaaatcaag
    24541 cagttataac cacatcatat gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg
    24601 ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt
    24661 gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcaac gcgcaccaaa
    24721 agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa atcacttaac
    24781 aacaggcggt aagcaaagcg aaattctgct accatccacg cactctttat ctgaataaat
    24841 ggcagcgact atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca gacctcacca
    24901 gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt
    24961 tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca
    25021 cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg
    25081 tcgcggcttt cctggtgacg acgaagaagc gcaacggcgg attattatgg cggaaatccc
    25141 ctcgccgctg ggcaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga
    25201 ccatccgata aaattcccgg caaaggcgca gttttatcag aatctgcaaa actacctgga
    25261 aaccgaactc aagcgtgaaa atccggtgct gattatgggc gatatgaata tcagtcctgg
    25321 cgacctggat attggcattg gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg
    25381 ttctttcctt ccggaggaac gcgaatggat ggagaggctg atgagctggg ggttggtcga
    25441 taccttccgc catgcgaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc
    25501 aaaaggtttt gacgataatc gcggtctgcg catcgacctg ctgctcgcca gccaaccgct
    25561 ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt cgcagcatgg aaaaaccgtc
    25621 cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agctgatctc ctggctcaaa
    25681 ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc
    25741 tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc tctattatca
    25801 agtttgatat aggaaactcc acgatgaacg ctgagcgtaa atttctttta gcctgtctta
    25861 tttttgcgct ggtcatttac gctatccacg ctttcggttt attcgatctg ctcaccgatt
    25921 taccccactt acagacactc attcgccaga gcggattgtt cggctatagc ctctatattc
    25981 tgttattcat cattgccacc ctctttctgt taccaggaag catactggtg atcgcgggag
    26041 gaatagtttt tggcccgctc ttagggacac tactatcatt aattgccgcc acgctggcct
    26101 cctcgtgctc attcctgctg gcgcgctggc tgggacgtga tttactgctg aaatacgttg
    26161 gtcatagcca tacctttcag gccattgaaa aaggcattgc gcgtaacggt attgattttc
    26221 ttattctgac ccgcttaata ccgttgtttc cttacaatat tcaaaattac gcttacggat
    26281 taaccacaat cgccttctgg ccttataccc ttatttcggc actcacgacc ctacccggta
    26341 ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta
    26401 ttttacaact ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct
    26461 acgcccgaca caaacatgtg gatctgtctg cttctcgccg cagcccactt tctcacccaa
    26521 aaaatgaagg atagaacgat gttgcaacat tattcagtgt catggaaaaa aggactggct
    26581 gcactctgct tactggctgt tgctgggctt agcggctgcg atcaaaaaga gaatgcggct
    26641 gcaaaagtgg aatacgacgg actttcgaac agccaaccgc tgcgtgtcga tgccaataac
    26701 catacggtaa ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac
    26761 ggtattgtgt ttaaagatgg ctccaacgga cataaatcgc tgtttatggg ttatgcgacc
    26821 ccgaaagcat tttatgaagc cctgaaagag gcaggtggta cgccgggcga aaacatgacg
    26881 atggataata aagaaacgac tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg
    26941 caaggggcgg caaaagcgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa
    27001 ctggacatgc gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg
    27061 gtgtgtctgg atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg
    27121 gtggaaaaac gcggcgaagt gaaattcaaa ggcaatgcct cagttctccc ggcggataac
    27181 acgctggcaa cggttacctt taaaatcacc gaataaagca ggataacgga tgatgaagat
    27241 gcaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt tcgctgtgct
    27301 gctggcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg ttgcggcgtt
    27361 tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg cactagcggc
    27421 agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac ctgcgttttt
    27481 gatcaccttt gccaatgcgt cgctgtttgg tgcgttctgg ggcggcttac tgtcgtggac
    27541 cagttcgatg gccggcgcgg cactgtgctt ttttatcgcc agagtgatgg gccgcgaagt
    27601 ggtggaaaaa ttaaccggca aaaccgtact tgacagtatg gacggctttt tcactcgcta
    27661 cggcaaacac accatcctgg tatgtcggtt attgcctttt gtccctttcg atccaatcag
    27721 ctatgctgcc ggtttgactt caatacgttt tcgctcgttt tttatcgcca ccgggcttgg
    27781 tcaattaccg gcgactattg tttattcctg ggcgggcagc atgttaacag gcggtacttt
    27841 ctggtttgtc accggactgt ttattctgtt tgccctgacc gtggtgattt ttatggcgaa
    27901 gaaaatatgg cttgagcgcc agaagaggaa tgcctgatgg gtttaccgcc gcttagcaaa
    27961 attcctttca ttttacgtcc gcagacgtgg ctgcatcgtc gccattacgg cgaggtgcta
    28021 agcccgattc gctggtgggg gcggatcccg tttatctttt atctggtgtc gatgtttgtc
    28081 ggctggctgg agcgcaaacg ctcaccgctc gatccggtag tacgatcgct tgtcagcgcg
    28141 cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat gaaagtcgcc
    28201 gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca aagcccgctc
    28261 tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt aacgccgcca
    28321 acggtcgatg atgccctgcg cgcccgactg gctacgcatt ttgacgctca ggcgctcacc
    28381 gaactgacgg cattgatagg cctgcaaaat ctgtcagccc gttttaattc tgccatggac
    28441 attcccgctc aggggctgtg ccgtattcct gaaaaacgtt cttaaggaga gaagatgcgc
    28501 ctttgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca tgccagtgac
    28561 tggcaggaaa taaaaaatga ggccaaagga caaaccgtct ggtttaacgc ctggggcggc
    28621 gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac ccattacgct
    28681 ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaaacg cattcagacc
    28741 gaagttgctg ccggacgtaa aacgggcggt tcggtggatc tgctctgggt gaacggcgaa
    28801 aacttccgca ccttaaaaga ggccaattta ttacaaacgg gctgggcgga gactctgccc
    28861 aactggcgct atgtcgacac acagcttccg gtgcgggaag atttttcagt gcctacagaa
    28921 ggggctgaat cgccctgggg cggcgcacaa ctgacgttta tcgcccgccg cgatgttacg
    28981 ccacagccac cacaaacgcc gcaagcctta ctggagtttg ctaaagccaa tcccggcacg
    29041 gttacctatc cccgcccacc ggactttacc ggcacggcgt ttcttgaaca gttgctgatt
    29101 atgctaacgc cagatcccac agcattaaaa gaagcgccgg acgatgcgac tttcgcccgc
    29161 gtcactgcgc ccttgtggca atatcttgat gcactgcatc cgtatttatg gcgcgaagga
    29221 aaggatttcc cgccttcgcc cgcgcggatg gatgctctgc tgaaagctaa cacattgcgc
    29281 ctgtcgctga cctttaaccc tgcacatgcg cagcaaaaaa tcgccagcgg cgatttgcct
    29341 gcaagcagtt acagttttgg ttttcgcgag gggatgattg gcaacgtcca tttcgtcacc
    29401 attcctgcca acgcgaatgc cagtgctgcg gcgaaggtag ttgccaattt cctgctctca
    29461 cccgatgcgc aactgcgtaa agcagatccc gctgtctggg gcgatccttc tgttctcgat
    29521 ccgcaaaaac tgcctgacgg gcagcgcgaa acattgcaat cgagaatgcc gcaggatctg
    29581 ccgccggtac tggctgaacc gcacgcaggt tgggtaaatg cgctggaaca agaatggcta
    29641 caccgttacg gtacgcatta atctttttgc tgtgggcgat gatagcggtg atttatgcac
    29701 cgctgatccc ggcagctctc acgctgatat cgcctgcttt gtcgttgacg cactggcagg
    29761 cgttatttgc cgatccacag ttaccgcagg cattactggc gacgctggtg tcgacaacca
    29821 ttgcggcggt cggggcgttg ttgattgccc tgctggtgat tgtggcgttg tggccagggc
    29881 cgaaatggca gcgtatgtgc gcccgtctgc cgtggctgct cgccattccc catgtggctt
    29941 ttgccaccag cgcccttcag ctctttgctg acggagggct gctttatgac tatttcccgt
    30001 atttcactcc gccaatggac aaattgggta ttgggctggg cctcaccctg gcggtgaaag
    30061 aaagcgcatt tctgctgtgg atcttagcgg cagtattgag cgaaaaacgg ctgttgcagc
    30121 aactcattgt gctggattcg ctgggctaca gccgctggca atgcctgaac tggttgctgt
    30181 tgccctccgt cgcgtctgcg ctggcaatgg cgatgctggc gattgtcgcc tggtcgctgt
    30241 cggtcgtgga tgtggcgatt attcttgggc caggtaatcc cccgacgctg gcggtaatta
    30301 gctggcagtg gttaactcag ggcgacgccg atcaacaaac gaaaggtgca ctcgccagcc
    30361 tgctgctgat gctgttactc gccgcctacg ttttgctggg ctatctgcta tggcgtggct
    30421 ggcggcgtac tattccccgc gtagatggcg ttcgtaagcc tgccacgcct ttattgccag
    30481 gcaatacgct ggcgagtttt ttacccttaa ccggtgtgct gtgtgtggtt ctgctggcga
    30541 tcctcgcgga tcagtcgacg atcaatagtg aagcgctcat caacagcctg acaatggggc
    30601 tggtggcggc attcatcgct ttgctcctgt tactgctgtg gctggaatgg gggccacagc
    30661 gtcgccagtt gtggctatgg ttgcccattt tattacccgc tctgccgctg gtggcgggcc
    30721 agtacacgct ggcgctatgg ctgaacctgg atggaagctg gacggcggtg gtctgggggc
    30781 atctgctatg ggtaatgccg tggatgctgt ttatcctgca acccgcctgg cagcgcattg
    30841 attcacggtt aattttgatt gcgcaaacac tgggctggtc gcgggccaaa atcttctttt
    30901 acgtaaaatg cccactcatg ttgcgccctg cgctgattgc cttcgcggtg ggattttcag
    30961 tcagtattgc gcagtatatg ccgacgctgt ggctgggcgc ggggcgtttt ccgacgctca
    31021 ccactgaagc ggtggcatta agcagcggcg gcagcaacgg tattctcgcc gcccaggctt
    31081 tatggcaact gctgttaccg cttattattt ttgccctgac cgcgttagtc gcaaaatggg
    31141 taggttatgt cagacaagga ctccgctaat gctctgcgtg aaaaatgttt cgctacgttt
    31201 accagaaagc cgcttgctga caaacgttaa ctttacagta gataaaggtg acattgtcac
    31261 gttaatggga ccatccggct gcgggaaatc cactctgttt tcgtggatga ttggtgcgct
    31321 ggctggacag ttttcttgta caggggagct atggcttaat gagcaacgga ttgacatgct
    31381 acccaccgca cagcgtcaga ttggcattct tttccaggat gcactgttat ttgaccagtt
    31441 cagcgtcggg aaaaatttac tgctggcgct accggcgaca cttaacggga atgcccgacg
    31501 taatgccgtg aatgatgcac ttgagcgttc aggccttgac ggagcgttcc atcaggatcc
    31561 tgccactttg tctggcggtc agcgagcgcg cgttgctctg cttcgcgccc ttctcgctca
    31621 gccaaaagcg ttgctactgg atgagccatt cagccgtctt gatgtgactc tgcgcgataa
    31681 ttttcgccat tgggtgttca gcgaaattcg cgccatggcg atccctgttg tgcaggtgac
    31741 gcacgatctt caggatgttc ctcctgatag ccccgttctg gatatggcgc agtggtcaga
    31801 aaattacaac aaactgcgat aacgcaaagt ttttcccgat gcgtcagttc agaatggcgc
    31861 tctcaaaact acaatgtcgg gattttcgat gaaacgtgtt tctcaaatga ccgcgctggc
    31921 aatggcttta gggctggctt gcgcttcttc gtgggccgct gaactggcga agcctcttac
    31981 acttgaccag cttcaacaac aaaatggcaa agcgattgat actcgcccca gcgcgtttta
    32041 taacggctgg ccacaaacct taaatggccc ttccggtcat gaacctgccg ccttaaacct
    32101 ctctgctagc tggctcgaca aaatgagcac cgaacagctc aacgagtgga tcaagcaaca
    32161 taacctgaaa gccgatgccc cggtggcgct gtacggtaat gacaaagatg tcgacgccgt
    32221 caaaacgcga ctgcaaaaag caggttttac gcatatctcc atcctgagtg acgcgctaag
    32281 cgaaccttcc cgtctgcaaa aactgccgca ctttgaacag ctggtatatc cgcagtggct
    32341 gcatgacctg caacaaggta aagaggttac ggcgaaacct gccggtgact ggaaagtcat
    32401 tgaagcggcc tggggcgctc caaaatttta ccttatcagc catattcccg gcgctgacta
    32461 catcgacacc aacgaagtgg aaagcgaacc gctgtggaac aaagtttctg atgaacaact
    32521 gaaagcgatg ctggcaaaac acggtattcg ccatgacacc acggtcattc tgtacgggcg
    32581 tgacgtatac gctgcagcgc gtgtggcgca aattatgctt tatgccggcg tgaaagatgt
    32641 gcgcctgctg gacggaggct ggcaaacctg gtccgacgcg ggtctgcccg ttgagcgcgg
    32701 aacgccaccg aaagtgaaag cggaaccgga tttcggcgtg aagatcccgg cacaaccgca
    32761 actgatgctt gatatggaac aagcgcgtgg actgctgcat cgccaggatg catcgctggt
    32821 gagcattcgt tcgtggccag aatttatcgg tacgaccagc ggttacagct atattaaacc
    32881 aaaaggtgaa atagccggag cacgttgggg acacgcaggt agcgactcga cgcatatgga
    32941 agatttccat aacccggatg gcaccatgcg cagcgccgat gatataaccg ctatgtggaa
    33001 agcatggaat atcaaaccag atcagcaagt ttcattctac tgcggcactg gctggcgcgc
    33061 gtcagaaacc tttatttacg cacgcgcaat gggctggaat aacgtctccg tttacgacgg
    33121 tggctggtac gaatggagca gcgatccaaa aaatccggta gcaaccggtg aacgcggccc
    33181 ggacagcagt aaataacatt gcaattactg acgctggagc gacttcagcg tcagataccc
    33241 actccacacc cgcgtaaagg tcgtcatcca gcacaacgca ccaaataccc acgcaaacca
    33301 cggaaaacag gccggaaaga ggcaactcag aacaaacagc aaaatcgtct cgctgccttc
    33361 ggttaacccg cccagataat aaaacgattt atgcgcataa ccaggattat caatctgatg
    33421 tttcgccgcc agcgcagcaa aagcgagaaa actgctgccc gtgccgataa acgcaaacag
    33481 cagccagccg cctaccaggg cgttttgctc cggtgcggcg aggataaacc caaaaggcac
    33541 cagcgcgtaa aaaagaaaat cgagagaaat atcgagaaag ccgcccgcgt cggtaagccc
    33601 tctgcgccgc gccagcgcac cgtccagacc atcaagcagc ctgttcaaca aaatggcgac
    33661 tagtgccgcc agataccagc ccagcgccag aaaaggcagt gccagcacgc cgatggcaaa
    33721 tcccactaat gttaaaccat ctggcgtaat gcccggttta tcaagaaccc gcacgcactg
    33781 atgcaacaac ggtttaatcc ggggatgaag atggcggtct agcacaggca ctccttaaat
    33841 ataaagcctt tctgattgag caacagtgcg gatattatgg catttttcgc ttatctgccc
    33901 gtgtgtaatt tatgaaaatg attgaagttg ttgccgccat catcgaacgt gatggcaaaa
    33961 ttttactcgc gcaacgccct gcccatagcg atcaggcggg attatgggag tttgccggtg
    34021 gtaaagtcga gccggatgaa agccagcggc aggcgctggt gcgtgagtta aacgaagaac
    34081 tgggcatcga agcaactgtt ggtgactatg ttgccagcca tcagcgagaa gtttcggggc
    34141 ggattatcca tcttcatgcc tggcacgtac ccgacttcca cgggacgtta caggcacatg
    34201 aacatcaggc gctggtctgg tgctcacctg aagaggcgct gcgatatccg ctggcccctg
    34261 ctgacattcc attattagag gcgtttatgg cttcacgcgc cgccagacca gcggattagt
    34321 gcttagggtt ttgtcatccc gttggcattg cagcagtatt ccttcggctt taattactgc
    34381 cccttcagaa taattttgat cctgataaac gcagcactga gtacagggct gcgctgactg
    34441 cccgcctgaa ctgaacactt ctggcggtac gtttacctcc acgtccggat gataatgcgg
    34501 gttagccagt gtaattaatg gaaatgctaa tactacggcg atcaatgctc gactcacagg
    34561 gaactcctta acgttattgt ctcttctaca gataacggta gccgggcggc aaaactttag
    34621 cgtctgagtt atcgcatttg gttatgagat tactctcgtt attaatttgc tttcctgggt
    34681 catttttttc ttgcttaccg tcacattctt gatggtatag tcgaaaactg caaaagcaca
    34741 tgacataaac aacataag
//
LOCUS       DAZFRE010000050        34635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFRE010000050 DAZFRE010000000
VERSION     DAZFRE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.050"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            51..791
                     /locus_tag="ACHKUI_002722"
     CDS             51..791
                     /locus_tag="ACHKUI_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6598893.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKNVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            826..3414
                     /gene="htrE"
                     /locus_tag="ACHKUI_002723"
     CDS             826..3414
                     /gene="htrE"
                     /locus_tag="ACHKUI_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO6598894.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFAVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAILNIVRSDGKNIPFAADIYDEQ
                     NNVIGNVGQGGQAFVRGIGQEGNIRITWIEEGKSVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            3431..4003
                     /locus_tag="ACHKUI_002724"
     CDS             3431..4003
                     /locus_tag="ACHKUI_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598895.1"
                     /translation="MIKTTPHKITILMGLLLSPSVFATDVDVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPNMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFSYQ"
     gene            4015..4623
                     /gene="yadL"
                     /locus_tag="ACHKUI_002725"
     CDS             4015..4623
                     /gene="yadL"
                     /locus_tag="ACHKUI_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO6598896.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGSAKLSAINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIELTPR
                     ALCEGNSNNGPGFANASTAAAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTDVTAGNFTAPATFTVTYN"
     gene            4650..5246
                     /locus_tag="ACHKUI_002726"
     CDS             4650..5246
                     /locus_tag="ACHKUI_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6598897.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQDPANTFNVLGATGRSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPTLNVTSGPLISHVLVDIYYE"
     gene            5272..6561
                     /locus_tag="ACHKUI_002727"
     CDS             5272..6561
                     /locus_tag="ACHKUI_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO6598898.1"
                     /translation="MQHDNGVKMKTFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSDKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINYHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLENTKSAQMVLKPNDSTSIYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            6673..7467
                     /gene="panB"
                     /locus_tag="ACHKUI_002728"
     CDS             6673..7467
                     /gene="panB"
                     /locus_tag="ACHKUI_002728"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6598899.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7479..8330
                     /gene="panC"
                     /locus_tag="ACHKUI_002729"
     CDS             7479..8330
                     /gene="panC"
                     /locus_tag="ACHKUI_002729"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6598900.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(8412..9296)
                     /gene="rpnC"
                     /locus_tag="ACHKUI_002730"
     CDS             complement(8412..9296)
                     /gene="rpnC"
                     /locus_tag="ACHKUI_002730"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO6598901.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGTPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMANLPLAEIDKMIN"
     gene            9570..9950
                     /gene="panD"
                     /locus_tag="ACHKUI_002731"
     CDS             9570..9950
                     /gene="panD"
                     /locus_tag="ACHKUI_002731"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6598902.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVNMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9954..11183)
                     /gene="yadE"
                     /locus_tag="ACHKUI_002732"
     CDS             complement(9954..11183)
                     /gene="yadE"
                     /locus_tag="ACHKUI_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6598903.1"
                     /translation="MYKQAVILLLMLFTASVSAALPAHYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11247..11687)
                     /gene="yadI"
                     /locus_tag="ACHKUI_002733"
     CDS             complement(11247..11687)
                     /gene="yadI"
                     /locus_tag="ACHKUI_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6598904.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11792..12562)
                     /gene="yadH"
                     /locus_tag="ACHKUI_002734"
     CDS             complement(11792..12562)
                     /gene="yadH"
                     /locus_tag="ACHKUI_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6598905.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12559..13485)
                     /gene="yadG"
                     /locus_tag="ACHKUI_002735"
     CDS             complement(12559..13485)
                     /gene="yadG"
                     /locus_tag="ACHKUI_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6598906.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13594..14256
                     /gene="can"
                     /locus_tag="ACHKUI_002736"
     CDS             13594..14256
                     /gene="can"
                     /locus_tag="ACHKUI_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6598907.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14297..14833)
                     /gene="hpt"
                     /locus_tag="ACHKUI_002737"
     CDS             complement(14297..14833)
                     /gene="hpt"
                     /locus_tag="ACHKUI_002737"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6598908.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15039..17429
                     /gene="gcd"
                     /locus_tag="ACHKUI_002738"
     CDS             15039..17429
                     /gene="gcd"
                     /locus_tag="ACHKUI_002738"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6598909.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDTKLDLVYLPMDVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17476..19026)
                     /gene="cueO"
                     /locus_tag="ACHKUI_002739"
     CDS             complement(17476..19026)
                     /gene="cueO"
                     /locus_tag="ACHKUI_002739"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6598910.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPVPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAVAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19192..19539
                     /gene="yacC"
                     /locus_tag="ACHKUI_002740"
     CDS             19192..19539
                     /gene="yacC"
                     /locus_tag="ACHKUI_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6598911.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19646..20512
                     /gene="speE"
                     /locus_tag="ACHKUI_002741"
     CDS             19646..20512
                     /gene="speE"
                     /locus_tag="ACHKUI_002741"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6598912.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20528..21322
                     /gene="speD"
                     /locus_tag="ACHKUI_002742"
     CDS             20528..21322
                     /gene="speD"
                     /locus_tag="ACHKUI_002742"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6598913.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21360..21722)
                     /gene="yacL"
                     /locus_tag="ACHKUI_002743"
     CDS             complement(21360..21722)
                     /gene="yacL"
                     /locus_tag="ACHKUI_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6598914.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(21897..24494)
                     /gene="acnB"
                     /locus_tag="ACHKUI_002744"
     CDS             complement(21897..24494)
                     /gene="acnB"
                     /locus_tag="ACHKUI_002744"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6598915.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            24849..26606
                     /gene="yacH"
                     /locus_tag="ACHKUI_002745"
     CDS             24849..26606
                     /gene="yacH"
                     /locus_tag="ACHKUI_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6598916.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHTAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(26848..28272)
                     /gene="lpdA"
                     /locus_tag="ACHKUI_002746"
     CDS             complement(26848..28272)
                     /gene="lpdA"
                     /locus_tag="ACHKUI_002746"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6598917.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28597..30489)
                     /gene="aceF"
                     /locus_tag="ACHKUI_002747"
     CDS             complement(28597..30489)
                     /gene="aceF"
                     /locus_tag="ACHKUI_002747"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6598918.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30504..33167)
                     /gene="aceE"
                     /locus_tag="ACHKUI_002748"
     CDS             complement(30504..33167)
                     /gene="aceE"
                     /locus_tag="ACHKUI_002748"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6598919.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33328..34092)
                     /gene="pdhR"
                     /locus_tag="ACHKUI_002749"
     CDS             complement(33328..34092)
                     /gene="pdhR"
                     /locus_tag="ACHKUI_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6598920.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(34548..>34635)
                     /locus_tag="ACHKUI_002750"
     CDS             complement(34548..>34635)
                     /locus_tag="ACHKUI_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO6598921.1"
                     /translation="EDGKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT         8755 a         9063 c         8306 g         8511 t
ORIGIN      
        1 tacttttgat aattattccc tgttttaatt aattctgtaa ggaatggttt atgtttttta
       61 atactaaaca taccgcagca ttgtgcttag cgagctgtat ggcttttagc tcatcagcta
      121 ttgcagacat cgtaatttca ggtacacgcg tagtttataa aagcgaacaa aaaaacgtca
      181 atgtacgtct ggaaaataaa ggcaataacc cattgctggt tcagagttgg ttagatactg
      241 gcgatgacaa cgctgaacca ggcagtatta ctgtcccctt taccgctacg ccgccagtat
      301 cacgtattga tgctaaacgt ggccaaacaa tcaaactgat gtacaccgcc agcagtgctc
      361 tgccaaaaga cagagaaagt gtgttctggt ttaacgtact ggaagtacct ccaaaaccag
      421 atgcagcaaa agcagcaaac caaagcctgc tacaactggc atttcgcaca cgtataaaac
      481 tgttctatcg cccggaagga ttgagtggaa tttcatctga tgcaccattg gctctgaagt
      541 ggtcctgggc aacatctgaa ggcaaagcag cattacgcgt tgataaccct acgccctact
      601 atgtctcttt tagcagtggt gatttagaag ccaacggcaa acgctacccc ctggatatga
      661 caatgattgc gccgttcagt aacgacgtca tcaaggtaaa tgggatgagt ggcagaacaa
      721 gctctgcaaa agtgcatttt ttcgccatta atgattttgg tggatctatt gaaggcaacg
      781 ccaaccttta atctggcaag gatataacac agcaggaagc agaacgtgac tattaaatct
      841 actaatcacc ttacccacat cgccacgttt tgcgctctgc tgtatagcaa tagtgcttta
      901 tgcgctgaac tcgtcgaata tgaccataca tttctgatgg gaaaggatgc ttcaaatatc
      961 gaccttagtc ggtatacaga aggcaatccc acactacctg gcatttacga tgtcagtgtg
     1021 tatgttaacg atcaacccat catgagccaa agcattgctt ttgctgttat tgaaggtaaa
     1081 aagaacgctc aggcctgcat tacgcagaaa aatttattgc aattccatat cagttcacca
     1141 gacaagaata gcgaaaaagc cattttgctt aagcgagacg aagatcttgg tgattgcctc
     1201 aatctggctg aaatgatccc tcaatcatcc attcgttacg atgttaacga tcaacgcctg
     1261 gatatcgatg tcccccaggc atggattatg aagaattatc aaaactatgt tgatccctct
     1321 ttgtgggaaa atggcattaa tgcagccatg ctttcctaca acctcaatgg atatcacagt
     1381 gaaagtccag gcagaacaaa tgacagtatc tatgcggcat tcaacggtgg gataaactta
     1441 ggcgcgtggc ggttacgtgc ctcaggtaac tacaactgga tgaccaatgt ccatagcgat
     1501 tacgattttc agaatcgcta tttacaacgc gatcttgcgt cactacgttc acaactggtt
     1561 attggtgaat cttacacgac cggcgagacc ttcgattcag tcagcattcg cggtattcgc
     1621 ttatacagtg acagtcgaat gctgcctccg gtattagcca gttttgcgcc aatcattcat
     1681 ggcgtagcga ataccaatgc caaagtaacc gttatgcaaa atggttataa aatctatgaa
     1741 acgacggtgc cgccaggggc ctttgccatt gacgatctta gcccgtcagg ttatggcagc
     1801 gatctcattg tgactatcga agaagctgat ggcacaaaac ggacgttctc tcagccattt
     1861 tcatctgttg tgcaaatgct gcgccctggc gttggtcgct gggatattag cgccggtcag
     1921 gttttaaaag acagtatcca ggatgagcct aatttatttc aagccagcta ctactatggt
     1981 ttgaataact acctgacagg atataccggt attcagctaa ctgataataa ctacaccgcc
     2041 ggattgctgg ggcttggtat gaataccccc gtgggggctt tttctgtcga tgttactcac
     2101 tcaaatgtca gcattccgga cgataaaacc tatcaaggac aaagctaccg tatttcctgg
     2161 aacaagttat ttgaaaatac cagtacatca ttaaatatcg cggcctatcg ctactccacc
     2221 cagcattatc tgggcctcaa tgatgcgctg acgctgattg atgaagtaga acatcctgag
     2281 caagatcttg agcctaaatc catgcgcaat tactcgcgca tgaaaaatca ggtcacggtc
     2341 agtatcaacc aaccgctgaa atttgagaaa aaggattacg gctcatttta tctctcggga
     2401 agctggtcag attactgggc ctccgggcaa aacagtacta attactctat tggttatagc
     2461 aatagtgcgt cctggggcag ttacagtatc agtgctcaac gttcgttgaa tgaagatggt
     2521 caaaccgacg atagcattta tctcagcttc acaattccaa tagaaaattt actgggtacg
     2581 gaacaccgca gctccggctt ccagagtatt gatacacaat taaacagcga ctttaagggt
     2641 aataaccaac tcaatatcag cagtagtggt tacagcgata ccaatcgtat cagctacagc
     2701 gtaaacacgg gttatatgat gaataaatcc agtgatgatt tgagttatat cggcggttat
     2761 gccagttatg aatcgccgtg gggaacgctg tcaggttcag cttcagcaag cagtgataat
     2821 agtcgccaat tctctctcaa tactgatggt ggatttgtgt tacatagcgg cggactgacg
     2881 ttcagtaatg acagttttag tgattccgac actctggctg tcattcaggc accaggagca
     2941 aaaggcgctc gtattaacta cggaaacagc accgtagacc gctggggtta tggtgttacc
     3001 agcgcccttt cgccatacca tgaaaaccgc atcgcactgg acattaacga tctggaaaac
     3061 gatgttgaat tgaaaagcac cagtactgtc gccgtcccgc gccagggagc cgttgtcttt
     3121 gctgattttg aaacagttca gggacaatcg gcaattctga atatcgtgcg aagcgatggt
     3181 aaaaatattc catttgcagc ggatatttat gatgaacaaa acaatgtcat cggcaacgta
     3241 ggtcagggtg gtcaagcctt tgttcgcggt atcgggcagg aaggaaatat acgaattacc
     3301 tggattgaag agggcaaatc ggtcagttgt tttgctcatt accaacaaaa cacgacatca
     3361 gaaaaaatag cacaatctat tattctgaac ggacttcgtt gtcagataca gtaaccacaa
     3421 gggacttcca atgataaaaa ccacaccaca taaaataaca atactcatgg gactattgtt
     3481 atcgccttcg gtatttgcaa cggatgttga tgtcgatttt accgctactg tgaaagcgac
     3541 aacatgtaac atcaccctga catcgctgaa tggttcaaac gtcgttgata atggcaatga
     3601 tagttatacc ctgataatcc ccaatatggg actggataaa atagtcaata aagcgtcaca
     3661 atctgaggcc aacttcaaat tagtcgccag tgggtgtagc agtggaatta gctggattga
     3721 taccacaatg actggaaatc agtctggtag tacatcgctg attattccac aatcttctga
     3781 ctcctcctca acaaccagta atattggtat gggatttaaa agattagaga cttcgggcga
     3841 tacgtttctg aaacccaata gcgcggaata catccgctgg aaaaccacag aaattagcac
     3901 cagtggtctg gaaatgactg tcgcactgcg cgaaacaacc ccaggacagg gcatacccgg
     3961 gaaatttcgc gcacaggcaa cgttcaattt cagctatcaa taacaaggat gacaatgatg
     4021 atattaaaaa aattatgtta tggattatcg ggtagtctat ttctggcttc atcactgtta
     4081 accatttcct cacatgcagc cactgacagt attggcctga ctgttactac tgacgtagaa
     4141 atgggtacct gtacatcaac gttgactgat gacggctcag ctaaactttc cgccatcaat
     4201 ttcggagatg tatatatttc tgaaattaat gccgaaacta aaattaaaac cttcaaatta
     4261 cagttcaaag actgtgcggg gattcccggg aaaaaggcca aaatagaatt gactccacga
     4321 gcgttatgtg aaggaaattc taataatggt cctggatttg ctaacgcatc aacggcagca
     4381 gcaaaagcag cggcggtcgc cgttgaggtc tggagcacat ctacaccagg taagaacggc
     4441 gctaaacaat tcagttgtgt cacccccgcg acagaagaag tcagtattgc taatgcgaca
     4501 ggaagtaatg ttgtcgatta cccaatgagt gcggtgttgg tggtcgcaaa agataaaacc
     4561 gttacagatg tcaccgcggg taatttcact gccccggcaa cattcaccgt gacttacaac
     4621 taacaggcag actgattcag gaactaaata tgcgtcccac tcaacgtaat ctgatgaaaa
     4681 aatcaattct gattctgtcg ttgctattca gcgccacttc ccctgtggtt gcaggacagg
     4741 atgttgacat tgttgcaaat ataaaaaata acacctgcca aagtggaatc agcaacaacg
     4801 gaaatattga tttaggtgtc gttggcgtgg gcgattttac cggaaacatt tcagccgaaa
     4861 attatcaccc cggtggaaaa gagttcacta ttacagtaca ggactgcaca cttcagggca
     4921 cagggaatgt gttaaatcag ttacatattg attttcgtgc actcagtggc gtaatggcga
     4981 caggttccag tcaaatattc gctaatgaag acaacgccgg ggctaaaaat gttggggtag
     5041 ttatattctc tattcaggac ccggcaaata ccttcaacgt tctcggcgcc actggtcgct
     5101 cacgttccgt ttatccggtc atgtcatcag cactaaacaa ttcatcctgg aaatttagca
     5161 cccgaatgca aaaaatcgat ccaacactga atgtcacctc tggcccactg ataagccatg
     5221 tgttagtgga tatctactac gaataacaga caaagtgcta caagtattat cttgcagcat
     5281 gacaatggag taaaaatgaa gacattcttc agatactttc tttttttagc actatgctct
     5341 tgttgttata cagcaagtgc cggcaccgat gataacgtca gctatcttgt tgggaatgat
     5401 tacggagtgg gtccgggaga ccaaaagtgg agagaaacag gccccagtgc agacattact
     5461 gtcaaattca ggtattcaac aagcacaaat aacttagttt tttataaacc aacccaactc
     5521 ggaccaacag gtgtgtcatt atactggtct cagttaaata gcgcttcagg tggaggttac
     5581 ctttactgca acagaagtaa tactgcaagc ggtgagccta tggcaattga aaatcaaatg
     5641 gttgattcgg gtaaacgtta tggcgggcat aaactatttg aaacatctgt ccctggcctg
     5701 tattatacat taaagatatc aaacatttgg tcatcataca ccacaaccaa tatcagttca
     5761 tcaggtatgt atatcggcga ttctccaact caatattttc ggtggatccc cgaaagtgaa
     5821 gccactctat attggtcttg taacaatgca aactcatctg ataaatactg ggccgtaggt
     5881 ggagtttggc agtcgctcac ggtggaattt tataccgata ccaactttga ccccacaaag
     5941 acaaacaagg taacattatc taagccgggt ggttatctct actcattcaa aggatatggt
     6001 cttggtggcg gtatcaacta tcacagctat ttccttaaaa tagattttga cctggataat
     6061 atgacgttat ccaacccaac gtgttttact gctgcactta ctggcccatc tgtaagcggt
     6121 tcaacagtta aaatgggaga ttatacccca gcgcaaataa aaaatggcgc aacagcggtt
     6181 ccttttgata tcacgctaca aaactgtatt cgtgttcgca atatagaaac aaaacttatg
     6241 tcaaacaagg tgggcagtgt aagcaaagaa cttctggcta acacactcac aggaaataac
     6301 gcagcaaaag gcgttggagt actgattgaa ggtttagaaa acactaaaag tgcacaaatg
     6361 gtactgaaac caaatgattc aacatctatt tataaagatt atgaaactga agatgacact
     6421 accgggggga tttacccgga taaaggcaac ggtacctcac aacctcttca tttccaggct
     6481 acattaaaac aagacggtaa tattgctata gaaccggggg attttaaagc caccagcact
     6541 tttcaggtta cctacccctg ataacactat cgcaccgcca gacaacggcg gtgcgatacg
     6601 gataaatcat tgcccccacc aatgtgactg ataaaatgcg agacagcaca acatcattta
     6661 tcaggatacg ctatgaaacc aaccacgatt gcttcattgc agaaatgcaa gcaggataaa
     6721 aagcgttttg cgaccatcac cgcttacgac tacagcttcg ctaaactgtt tgccgaagaa
     6781 ggacttaacg tcatgctggt aggcgattca ctgggcatga cggttcaggg gcatgactcc
     6841 acgctaccgg ttaccgttgc tgatatcgcc tatcacaccg ccgctgtgcg ccgcggtgcg
     6901 ccaaactgcc ttctgttagc agacctgcca tttatggcgt atgccacgcc ggaacaagcc
     6961 tttgaaaacg ccgcaacggt aatgcgtgcc ggagccaaca tggtcaaaat tgaaggcggt
     7021 gagtggctgg tggaaaccgt acaaatgctg accgaacgtg ccgttccagt ttgtggtcat
     7081 ttaggcctaa ccccacagtc agtgaatatt ttcggtggct acaaagtcca ggggcgcggc
     7141 gatgaagcgg gcgatcgact gctcagcgat gcattagcct tagaagctgc tggggcacaa
     7201 ctgctggtac tggagtgcgt gccggttgaa ctggcaaaac gcattaccga agcactggcg
     7261 atcccggtta tcggtattgg cgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     7321 gacgcgttcg gcattaccgg cggtcatatt cctaaattcg ccaaaaattt cctcgccgaa
     7381 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttac
     7441 ccgggcgaag aacacagttt ccattaagga gtgacgttgt gttaattatc gaaaccctgc
     7501 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg taagcgcgtg gcgctggtgc
     7561 ccaccatggg taacctgcac gacggccata tgaagctggt cgacgaagcc aaagcccgcg
     7621 ccgatgtggt cgtcgtcagt attttcgtta acccaatgca gtttgaccgc ccggaagatc
     7681 tggcacgtta tccacggacc ttgcaagagg actgcgaaaa gctgaacaaa cgtaaagtgg
     7741 atttagtttt cgcgccttcg gtaaaagaga tctacccgaa cggtactgaa acccacacct
     7801 atgttgatgt tcccggtctt tccaccatgc tggaaggtgc cagccgtccg ggacattttc
     7861 gcggcgtttc gactattgtc agcaagctgt tcaacctggt ccagccagat atcgcctgct
     7921 tcggtgaaaa ggattttcag caactggcgc tgatccgcaa aatggttgcc gatatgggct
     7981 tcgatattga gattgtcggc gtaccgatta tgcgcgcgaa agacggtctg gcgctcagtt
     8041 cccgtaacgg ctatctgacg gcggaacaac gtaaaattgc gccgggtctc tacaaagtct
     8101 taagttcaat tgccgacaaa ttgcaggctg gcgaacggga tctcgatgaa attattgcta
     8161 ttgccgggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgtgatg
     8221 ccgacacatt gctggaagtc tctgaaaaca gcaaacgcgc agtgattctg gtagccgcct
     8281 ggcttggcga tgctcgcctg atcgacaaca aactggtcga actggcgtaa tgcttaactg
     8341 gcgctacggc tgatggcgcc agttattcat tgccacgtta tctgtaggca attattcata
     8401 atgcagacaa cttagttaat cattttatca atctcagcaa gaggtaaatt tgccatctct
     8461 gcaacgtctt cccgagacat tcctttactc agaagacgca gggcgaattc ctgacttact
     8521 tcctgtcttc cctgctgaat ccccttctct tcaaaccact gcgccagcgt catcatagac
     8581 tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
     8641 ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt gtacccttcg
     8701 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
     8761 tgcagcagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgata
     8821 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagttc cggtgagtaa
     8881 aacatatcaa accagcacat tgatagcgga taaggtgtgg cctcgccctg ataaaacaga
     8941 attggcacca ccagcggcag cttatcgtgg tcagcctcca gatgccggtg catggcagca
     9001 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg atgttcaatc
     9061 acaacatgca aataaccggg ggtgccctgc atttgcacgg aataaagcac gtcagtgctg
     9121 tgccctttca ggctttcttc aataaaactc cccgactcaa gatgcagcgt gtcgaggtca
     9181 cacagttcgc gtagctccgc tggcaaatgg atatccagaa agtcgcgagc cgtttccgca
     9241 tgcattaaaa attgtttaaa caccgcatca tgcggtgtgg tactcggtgc atccatcttc
     9301 cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgcac cgtcgtcagc
     9361 cgcctcttta gtgtttaccg agcagcgttc agagaaatat caattcgtta caggcgttac
     9421 atggcacgct tcggcacggt acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca
     9481 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat cgcctgcttc
     9541 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
     9601 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
     9661 attttcttga cgcagctggt attctcgaaa acgaagccat tgatatctgg aatgtgacca
     9721 acggcaagcg tttctccacc tatgccatcg cggcagaacg cggttcgaga attatctccg
     9781 ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg
     9841 ttaacatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
     9901 acaacgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcctga ttgttatccc
     9961 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    10021 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggtcgtcacc
    10081 gccaggtgaa atccggcttc ttttgctgcc ttcacggctt tgtcattaaa tccgccaaat
    10141 ggataagaaa gatacaaaac atgtggatta aattgcgcca gagcgcggcg tgaacgtgca
    10201 aaatcaaaca gaatattgtg ctcactacgg ctcagcaata taggccgccg ataaccatct
    10261 atccgatgca aaaaatgagt atgtgactgg aaatcaaata catcgcgaat ttcgttaagt
    10321 tcagaaacgc tcataaattg cagcgatttt gggttccatt tctgcgggtg acgtttgatg
    10381 cgcgaggtaa caataaacgc cgtcgccttc ataccatatt gtttcaacac aggatacgca
    10441 tagcggctca ccgacttgag gccatcatca aaggtaatta ccaccgctcg cgcagggaga
    10501 ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc gtatcccctg
    10561 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    10621 cgaaaacggg tgttttcttc gtcgcgcaaa atatggtgat aggttagcac cggcaagcca
    10681 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
    10741 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    10801 gccagtactc caaatggcgc actgcccaca ctcggtgcgt tataaaccgg cgtatctttc
    10861 caggttatta agttctgatt actcagcggc ttgttgaggt cacccaaacc atcttcaact
    10921 ttttgtcgcc cctgaaccgg ctcgagatgg cctttatcga taaacccttt gccaaagcca
    10981 aaattaaata cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    11041 atattcccca ccgtcaccat cttgtcacca atttgcgccc agaccgcggc attttcgatg
    11101 gtttgcatat aatgggcagg taatgcggca ctgacgcttg cggtaaacag catcagcagg
    11161 agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg cttttattgt
    11221 agcaaaagcg tcatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    11281 ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcgcacc cagttcgaca
    11341 atatgctcgc gaaacgctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    11401 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    11461 gccacgcggt acggtggcgc tccggctata tcggttaaaa agataacgcc ctcaccagag
    11521 tccgtttcat gcaaggcatc gcacatcatg cggctgagca tattagagct taatccgcgc
    11581 cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag cgcatccagt
    11641 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    11701 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    11761 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    11821 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    11881 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    11941 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agggagtaaa
    12001 acaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    12061 cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc accgcagtga
    12121 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    12181 aaattgccgt caccaaaata ccaacaaaca gaccacgcgc cacgccacca ccgacatatc
    12241 cggcaataat gacatgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    12301 acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc accgacatca
    12361 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    12421 aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact ggcggcacca
    12481 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    12541 ccacccagta aagatgcatc atgtgcgatc tccttgtttt tcattaacca gtgaaacaaa
    12601 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    12661 taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt ccagcgtggc
    12721 ggtatctacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    12781 atcgagaata aaggtttctg atttcagctt cgccagcagc gccttcatcg aagtattttc
    12841 caccaactca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    12901 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    12961 cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt
    13021 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    13081 acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatact tttcgctgcg
    13141 aatgtacgct tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    13201 cacggtttca aacgggttga agttaaattc ctgtggcacc agacccaact gacgtttagc
    13261 gtttacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    13321 taccagagag ctgataatac cgatagtggt cgattttccg gccccgttcg gcccgagaag
    13381 cgcataaaaa tcacccgctt cgacctgcaa atcgattcca cgaagcgcct gaacgccgcc
    13441 tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    13501 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    13561 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    13621 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    13681 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    13741 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    13801 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    13861 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    13921 aacccagaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    13981 catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    14041 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    14101 cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt
    14161 gatctggatg tcacagcgac taaccgcgaa acccttgagc aacgttaccg tcacgggatc
    14221 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
    14281 tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac ggcagatgac
    14341 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    14401 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    14461 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    14521 ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat
    14581 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    14641 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    14701 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    14761 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    14821 cagtatgttt catatctata accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    14881 ttcagcaccg caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga
    14941 atctgttaat aaagctcgaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    15001 gctactaaaa tattaatgaa ttgaaatggt gtctatttat ggcaattaac aataccggct
    15061 cgcggcgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    15121 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    15181 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctgt
    15241 acgcagccct gctgctcggc actatgattt ggggcgtctg ggaagttggt ttcgacttct
    15301 gggcgctgac tccgcgcagc gacattctgg tcttcttcgg catctggctg atcctgccgt
    15361 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac
    15421 tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag gaaatcagcg
    15481 gcaccttaag cgccgatacc acgccagcag aagccatctc ccccgttgcc gatcaggact
    15541 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc accactgaag caaattaacg
    15601 ctgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    15661 cgaacgatcc gggcgaaatc accaatgaag tgacgccaat taaagtgggc gacacccttt
    15721 acctgtgtac cgcgcatcaa cgtctgtttg cgctggatgc cgccagcggt aaagagaaat
    15781 ggcattacga tcctgagctg aaaaccaacg agtccttcca gcatgtaacc tgccgtggcg
    15841 tctcttatca tgaagccaaa gcagaaactg cttcgccgga agtgatggcg gattgcccgc
    15901 gtcgtatcat tcttccggtc aatgatggtc gtctgattgc gattaacgct gaaaacggca
    15961 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccggaca
    16021 ccaaaccggg tctgtatgag ccgacttcgc cgccgattat caccgataaa accatcgtga
    16081 tggccggttc agtcaccgac aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    16141 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaatg
    16201 cgatcccgtc tgacgaacat acctttacct ttaactcacc aaactcgtgg gcaccagcgg
    16261 cctatgacac gaagctggat ctggtctatc tgccgatgga cgtgaccacg ccagatatct
    16321 ggggcggtaa tcgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    16381 ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg tgggatatgg
    16441 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccggtca
    16501 tttatgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat ggtgaactgg
    16561 tggttcctgc cccggaaaaa ccggttcccc aaggtgctgc taaaggcgat tacgtgaccc
    16621 ctactcaacc cttctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    16681 tgtggggagc caccatgttt gaccagttgg tgtgccgcgt gatgttccac cagatgcgct
    16741 atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg
    16801 ggatgttcga atggggcggg atttccgttg atccgaatcg tgaagtggcg attgccaacc
    16861 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tcctggtaac ccgatggaac
    16921 agccgaaaga tgccaaaggc acgggtacgg aatccggcat ccagccgcag tacggtgtac
    16981 cgtatggcgt cacgctcaac ccgttcctct caccgtttgg tctgccatgt aaacagccag
    17041 catggggcta tatctcggca ttggatctga aaaccaatga agtggtgtgg aagaaacgta
    17101 ttggtacgcc gcaggacagt atgccattcc cgatgcctgt accggtgccg ttcaatatgg
    17161 gtatgccgat gcttggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    17221 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    17281 gtttaccagc gggtggtcag gctacgccaa tgacctatga agtgaatggc aagcagtatg
    17341 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg
    17401 cttatgcgct gccggatgat gtgaagtaac cataatgccc ggagagatcc gggcatattt
    17461 ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag
    17521 cagatggcag tgcgccatat aagcatgttc tttcggtgca tcgtgattaa acttcaccag
    17581 cacttcgctg acattacctt ctaccttaac ggtatctttc cagcctgcgc gatgcgtcgc
    17641 tggcggtttg ccattttctg acaagatacg gaactgcgta ccgtggatat ggaacggatg
    17701 cagcatcatg tcgcctacgc cagagataac ccaacgttcg tactgccctt tcgccaccgc
    17761 aaacatcggc ttattcatat caaacgcctg accgttgatt ttgttggcat ggtggaaatc
    17821 aaacttcccg ctgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg
    17881 gctgtgatcc atcccggcca tcgcctgatc gccatatttc tccatcagca tctgcatccc
    17941 catcatatcg agcattgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag
    18001 cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa gcaccagagg cactaatggc
    18061 aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg ccatccccat
    18121 ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac
    18181 ttcaaaacgc tcgcccatca gcactggcag ctcgttcacc ttcaccggta caggtagcag
    18241 accgccgtcg ctggcaatca cataaagcgg gcgattgtcg ctggtggcga aattgagcga
    18301 gcgggcatta cagccattga gcaaacgcag gcgcagccag ccacgcgggg cagcgtgttg
    18361 cgggtagatt acaccgttgg tcagcaacgt atcgccaaac caacccacag cggcggtcat
    18421 cacatccagt tgataatcaa tctgcccgtc agcgttaaat tttttatcct gaacaatcac
    18481 cgggacatca tcaatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc
    18541 aatcaccacc aaaccagcca gccccatcgc cacctgacgt ccggtcttgc catgttgatg
    18601 tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc
    18661 acctggcgga ataattccct gcgggccgcc gtccacttca cccggtactt ccagcccgtg
    18721 ccagtgcaac gtcgtctctt ccgtcagttg gttgtagata tcaaccgtta ccgctttgcc
    18781 acgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcggt
    18841 tttcccgcca aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc
    18901 ggtcgtgagc aaatcaggga ttggtaacgt tgggcgttcc gccgcaaata ctgcgcggct
    18961 ccacagcggc aaggctgaag ccacacccag cgcgacggaa tatttcaaga aatcacgacg
    19021 ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc
    19081 cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc
    19141 ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg
    19201 tttttcagaa cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc
    19261 agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtttt tctgaagaac
    19321 gattgtggtt accagaactt acctaacggg caaattcgtc gcgcgctggt ctttttcgcc
    19381 cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc cctcggtgaa
    19441 gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg caaagccctg
    19501 gctcgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga
    19561 aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccatt ttttttacgg
    19621 gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca
    19681 cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa agaccgatca
    19741 ccaggatctg atcatttttg agaacgccgc atttggtcgc gtaatggcgc tggatggtgt
    19801 agtacaaacc accgagcgtg acgagtttat ctatcatgag atgatgaccc atgttccgct
    19861 actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg gtgccatgct
    19921 gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg
    19981 tgtcgtgtcg ttctgccgtc agtatcttcc caaccataac gccggtagtt acgacgatcc
    20041 gcgctttaaa ctggtgatcg acgatggtgt caatttcgtt aatcaaacca accagacctt
    20101 tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagtc tgtttacttc
    20161 tgcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa
    20221 cggcgtctgc tttttacagc aggaagaggc catcgacagt catcgcaaac tcagccatta
    20281 cttcagcgat gttggctttt atcaggcggc gatcccgacc tattacggcg gcatcatgac
    20341 ctttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa ttattcaggc
    20401 gcgttttctc gcttctggcc tgaaatgccg ttattacaat ccggcagtcc atacggcagc
    20461 ttttgcctta ccccagtatc tgcaagatgc actggcttca cagccgtcct aaggagaaga
    20521 taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa gtctgagttt
    20581 ttgtatttac gatatctgct acgccaaaac taccgaagag cgcgacggtt atattgctta
    20641 tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat
    20701 tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg gtgccagcgt
    20761 cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa cagaacaccc
    20821 cggcccactg ccagaaacgg tcgttgccca tctcgataaa agtcatattt gcgtacatac
    20881 ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata ttgaagtctc
    20941 tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc agcttgagtc
    21001 cgatattgtg actattgatt atcgcgtgcg cggttttacc cgcgacatta acggtatgaa
    21061 gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtccgaag atatgaaggc
    21121 gctgtatgac atggtggatg taaacgtcta tcaggaaaat atcttccata ccaagatgtt
    21181 gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag atttaaccga
    21241 cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg
    21301 gcgcaatatg cccgctgttt aacgtctcag gcggagcacg gaggctccgc ctgatttact
    21361 tacttctgct gcacgaaatt acggtaagcc gccaccacct gaagaaaatc ctcaacgccg
    21421 cacagcgaca agctctcttc atcgtagtag ttcatcccct cttccatttc atcgccagcg
    21481 aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag agtgtattca
    21541 tgccctgccc gctgccagga tcgttcgcta cctttcaatg cgtgcgccgc ttgttccact
    21601 tcatcaagca aggccaggtt ttctttcacc tcttcattaa accagtgccc gaccacttca
    21661 tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc
    21721 atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgccat tgatgtaatt
    21781 atcgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata aaaaatgccc
    21841 gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct gactttttaa
    21901 accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag ctggttgaag
    21961 ttcagataac ggtaagtatc aacggcagtt ttatctactt gcgccacgta ggtctggtac
    22021 tcttccggcg tcggcagttt gccaatcagc gccgcaacag cagccagttc cgcagaagcc
    22081 aggaagacat tcgcgcccgt ccccaaacgg ttcgggaagt tacgggtaga ggtggaaacc
    22141 accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagggaaca gccagggatc
    22201 tcgatacgag caccactctt accgaagacg ctgtagtagc cttcttcggt cagctgtgca
    22261 gcgtccatac gcgttggcgg tgccacccac aggcgggtcg gcaactgacc tttgtgcgca
    22321 tccagcagtt tacccgcagc acggaagtga ccgatgttag tcatgcaaga accgataaac
    22381 acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gagcgtcgtc cgggtcgttc
    22441 ggtgcacaga ggattggctc tttaatatcc gccagatcga tgtcgatcac tgccgcgtat
    22501 tccgcatctg catcggcttc cagcagctca ggattcgcca gccatttttc catgccctga
    22561 atacgacgtt ccagggtacg acgatcgccg taaccttccg cgatcatcca cttcagcagg
    22621 acgatgttag agttcaggta ttcgatgatc ggttctttgt tcagcttgat ggtacaaccg
    22681 gcagcagaac gctcggcaga cgcatcggtc agctcaaatg cctgctcaac tttcagatcc
    22741 ggcagacctt caatttccag aatgcgacca gagaagatgt ttttcttgcc tttcttctca
    22801 acggtcagca gaccttgttt gattgcatac agcggaatgg cgtgaaccag gtcgcgcagg
    22861 gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg catatccagc
    22921 ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg gaaagagata
    22981 ccgatcggga aacgggtatg ggagtcaccg cccgtaccga cagtatccgg cagcagcata
    23041 cggttcagcc aggagtggat tacgccgtca cccggacgca gcgacacacc gccacggttc
    23101 ataatgaagt ccggcagcgt gtggtgcgta ttcacgtcaa ccggtttcgg atatgccgcg
    23161 gtgtggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc caggtctttc
    23221 agttcatcac gggtcatcgg gccggtggtg tcctgggaac ctacagaagt cattttcggt
    23281 tcacagtacg cgcccggacg aatgcctttc acaccgcagg cacgacctac cattttctgc
    23341 gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa cacatcacta
    23401 tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat aatcagcgga
    23461 atacggccgc cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc gaaggttgcc
    23521 agcagttcgc cggtttcgtg gttacgcact tcacctttgt acgggtaaac gtcaatcacg
    23581 tcacccatgt tcaggttaga gacgtccact tcgattggca gtgcacccgc atcttccatc
    23641 gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc
    23701 ggcacatgtg gaatatcatc acccataaac cacagtacgg agttcgtggc ggatttacgc
    23761 gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga
    23821 gcttcaatct gtttgatcgg accaacaaca ccaggctgat ctggctcaat accttcacgg
    23881 gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca cgcatccggt
    23941 gccggagaaa ggtcatcggt gttggtttcg cctgtgactt tgaagacggt aacggtcagt
    24001 ttttcagcca gtgccgggcg attcaggaac cattcggcat ctgcccagga ctgcattacc
    24061 tgcttcgcat attcgttacc tgctttcgct ttctcttcta cgtcatagaa gttatcgaac
    24121 atcagcagcg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc atcatccagc
    24181 gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tgcccagcag ttcgatggct
    24241 ttttccggag tcagcagtgg ggatttggct tcgcctttcg cgacagcagc caggaagcct
    24301 gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa cagatctaac
    24361 aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc ggccatttgg
    24421 tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac gtgcttacgg
    24481 tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca ggcgattctc
    24541 ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa
    24601 tagcaggatt ttgacgatgt gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg
    24661 aatcgttaag gatggtctag agaatgtttc cctccgtaaa aacttcgtta attcgcatgg
    24721 tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta cggcctcttt
    24781 atactcggat tcacagcacc tgcgggtggc agttcgccga ccattgcgat ttccttgaga
    24841 tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta atttgcagtg
    24901 ccgggctttg tgccgcctct accgggctat atataaaaag ccgcacagtg gaagcgcctg
    24961 tggaaccgca atcgacacaa ctggctgcgc ctgacatctc cgcagttacg cttcctgcaa
    25021 cggtttccgt gcctcctgta acacctgccg tcgtcaaatc cgcattcagc actgcgcaaa
    25081 tagatcaatg ggttgcacct gtcgcgctat accccgactc tctgctttca caagtgttaa
    25141 tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac gataatccac
    25201 ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccctgggac gccagcgtca
    25261 aatcactagt ggcctttcca cagttgatgg cattgatggg cgaaaacccg caatgggtgc
    25321 aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg gtacagcgat
    25381 tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aactgaacaa aagatcatca
    25441 cgacaacgaa aaaagtcgta ccggtaaatc agcccgccaa cgcccctgcc acacaatcaa
    25501 ataccgtttc tacgtccagc cccgtcgtcg cagaacctgc accgaccgta ataaccattg
    25561 agccagccaa tccggatgtg gtttatattc cgaactacaa cccaaacgtg gtttacggca
    25621 gttgggccaa taccgcttat ccgccagttt atctgccgcc gccagccgga gaaccgtttg
    25681 ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccaca tacgcactat
    25741 tcagcagcat cgactgggac gacgacgatc atgaccatca tcatcatgac gatgatgatt
    25801 atcatcacca cgatggcggt catcgtgacg gtaatgactg gcaacacaac ggcgacaaca
    25861 tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact gataagaata
    25921 tggcatggcg gcacaatcca aactaccgta atggtgtgac ctatcatgat caggatatgg
    25981 caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag cgccacgcag ttacctgctc
    26041 caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga acacacaccg
    26101 cccccgtcat tacgcgagat acccaacgtc aggcagcggc acagcggttt aatgaagctg
    26161 aacacaatgg gagctatgac gacttccgcg acttcagccg tcgccagcca ctgacccagc
    26221 aacaaaagga cgccgctcgt cagcgttatc agtcggcttc tcctgagcag cgccaggcgg
    26281 ctcgcgagaa aatgcagact aacccacaga accagcagcg aagagaggcg gcacgtcagc
    26341 gtattcagtc cgcgtcgcct gagcagcgcc aggcggttcg cgagaaaatg cagactaacc
    26401 cgcagaatca acagcaaaga gatgcagcac gtcagcgtat ccagtctgcc tcgcctgagc
    26461 agcgtcaggt ttttcgagag aaagttcagg agagccgccc gcagcgtcta aacgacagta
    26521 accatactgc ccgattgaat aatgagcaac ggtcagcggt acgcgaacgg ttgtctgagc
    26581 gtggagcaag gagactggaa aggtaaattg caggcgtaaa aaagcggcgt tgttagccgc
    26641 ttaagactga tgacaaacat cgaatcgccc gatgcgcaag cttatcgggc ctacacaagc
    26701 catgcaatat attgaatttg catgattttg taggtcggat aaggcgttta cgccgcatcc
    26761 ggcatcaacc acgagcactt tatcaacaat ctgaagcggc cagaatagcc gcttcttaat
    26821 ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc
    26881 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    26941 cgcatggatg gtcagtgcga tgtcttcagc gtcacaaccc atttcgattg ccaggccgat
    27001 ttcacccagc agctcaccgc cgttagtacc gacaattgca ccaccgatca cacggtgaga
    27061 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcggaagcga tagcacgacc
    27121 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    27181 ctcagtcaga ccaacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    27241 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    27301 acctttatgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac
    27361 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc
    27421 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca attgctacca gcacggcgtc
    27481 gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa tgccgtcttc
    27541 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaattttt tgctgatacg
    27601 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    27661 aaccacatca atctgtgaac ccagcgcgtg gtatacggtg cccatttcca gaccgatgat
    27721 accgccaccc attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga
    27781 gtcccagata cgcggatctt catgcggaat aaacggcagt tgaatcgggc gagaacccgc
    27841 tgcaatgatc gcgttgtcga agttgatcac ggttttaccg ttctcacctt caacttccag
    27901 ggtgttagcc ccggtaaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    27961 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    28021 gtcgatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    28081 ttcgatgact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    28141 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    28201 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    28261 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    28321 ggccgcagcg gcgttagctt cacgccctcc ccccagtcac ttacttatgt aagctcctgg
    28381 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    28441 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    28501 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    28561 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    28621 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    28681 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    28741 caccggctcc atcgcggact tggaaacgcc aaggatagcc acttccggcg cgttcacaat
    28801 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    28861 catttcgccc gcagtcagct taccgtcacg cgctttcttg gaaatagtca tcagctcgcg
    28921 agacaactcg atgatgcctt tcttattgac gtctttgaat accggaacaa ccagaccgtt
    28981 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    29041 ttccgacagc gaactgttaa agcgaggcat ctgctcaaga gctgcagcaa ccgctttcat
    29101 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    29161 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtgag taacatgcgg
    29221 gatcatcacc cagttacggc tcaggttcgc accagagatt ttctggatgc ggcccagttc
    29281 cacttcttcg atttcaccaa acttgctgaa gtccacttta ggccacggca gcatgcctgg
    29341 gataccgccg ccagttgccg ccggagccgc ttctgcacgt ttgatagctt ctttcacgta
    29401 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    29461 gttaacgcca aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa cgtaagcgtc
    29521 gttttcagca aattcagatt tgccttccgc tttcgcagcc ggtgctgctg ccggggcttc
    29581 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggcgc
    29641 tgcgccttca acttcgaaga tcataattag cgaaccagtt ttcactttat cgccaacgtt
    29701 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag acgctttatc
    29761 gccttctacg gtgatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    29821 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    29881 cgctggtgca ggagccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc
    29941 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    30001 gttcactttg atctctttca cggtgcctgc aaacggagcc ggaacttcca tagaagcctt
    30061 gtcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    30121 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    30181 tgcagccgct ggtgctgctg ccggagcggc ttctttcttc tcttctgcct gagcaggtgc
    30241 agcgtcagct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    30301 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagacg gaacttccat
    30361 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    30421 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    30481 gatagccatt attcttttac ctcttaagcc agacgcgggt taactttatc tgcatcgatg
    30541 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    30601 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    30661 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt
    30721 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccgga
    30781 gcatcgttca tcacctgagc gatgtatgga acgcgcggag tttccagcgg gtgcagcatg
    30841 ttccagcgtt cacaatcctg accatcacgc gccagctctg tgaaggaggt cacgctataa
    30901 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    30961 aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    31021 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    31081 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    31141 tccagaccgt catgcatgat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    31201 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttcg
    31261 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg
    31321 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    31381 aacggaatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacac
    31441 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    31501 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    31561 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    31621 agacgatctt tgatcgactt gttcttcagc atcacgttca gggcacgaac gaaagcgata
    31681 gtggtagaga tctctttgct ctgctcttcc aacagcgcgc cgaagtcttg caggctcggc
    31741 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    31801 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    31861 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    31921 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    31981 ccgtaacctt taatggtatg agcaaggatt accgtcgctt tgcctttggt ttcctgcgct
    32041 ttcttaaatg cagcgtagat tttcttcgga tcgtgaccgc cacggttcag ggcccagatc
    32101 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    32161 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    32221 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    32281 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    32341 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    32401 aagaccaagt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    32461 tcgtccattt caccgtcgcc gaggaacgcg taaacggttt gtttagaggt atctttcagg
    32521 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    32581 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    32641 gagaggccat tgccgtgaac ttcctgacgg aagttatcca actgctcctg agtcagacga
    32701 ccttccagga aagcacgagc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg
    32761 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    32821 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    32881 cgcagaaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    32941 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    33001 ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    33061 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    33121 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    33181 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc
    33241 aataagacag gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct
    33301 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgtagaga
    33361 acgctcacgg cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    33421 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    33481 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    33541 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    33601 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcat ttgattccgc
    33661 gtccagatcg ccagactgct gcgccagctc tatggcatgg tggagttcac ggatgcgttc
    33721 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    33781 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    33841 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    33901 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    33961 gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    34021 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    34081 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    34141 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    34201 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    34261 tatgcacaaa tgttaaattt acccatcaag gggtgagatt aacagaattg aaacgaaaaa
    34321 atatgtcctc tgaaccgatt caccaagcaa caaaacgcat aacaattcaa ttatgtgaat
    34381 taatatgtaa aatgaatgtt cagggaagga gagaaaggta gtatttacct atgttttctt
    34441 tttacattgc ttattatcac tgcaatcgcc accgtaacct tctcgtagcg aaaatcatca
    34501 cttaaccaga acgtgatgat tttcaggtga cagattctgt ttagaattca ttctgaatct
    34561 ttgaacaaag acaatccttg cgaacgccgc cattctttga cgattttcag gatgttttca
    34621 ggtttaccat cttct
//
LOCUS       DAZFRE010000051        33701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFRE010000051 DAZFRE010000000
VERSION     DAZFRE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.051"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(78..2147)
                     /gene="glyS"
                     /locus_tag="ACHKUI_002751"
     CDS             complement(78..2147)
                     /gene="glyS"
                     /locus_tag="ACHKUI_002751"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6598922.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2157..3068)
                     /gene="glyQ"
                     /locus_tag="ACHKUI_002752"
     CDS             complement(2157..3068)
                     /gene="glyQ"
                     /locus_tag="ACHKUI_002752"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6598923.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3163..3462)
                     /gene="ysaB"
                     /locus_tag="ACHKUI_002753"
     CDS             complement(3163..3462)
                     /gene="ysaB"
                     /locus_tag="ACHKUI_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6598924.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3637..4632
                     /gene="wecH"
                     /locus_tag="ACHKUI_002754"
     CDS             3637..4632
                     /gene="wecH"
                     /locus_tag="ACHKUI_002754"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6598925.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4673..5110)
                     /gene="yiaA"
                     /locus_tag="ACHKUI_002755"
     CDS             complement(4673..5110)
                     /gene="yiaA"
                     /locus_tag="ACHKUI_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6598926.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5156..5497)
                     /gene="yiaB"
                     /locus_tag="ACHKUI_002756"
     CDS             complement(5156..5497)
                     /gene="yiaB"
                     /locus_tag="ACHKUI_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6598927.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5666..7120)
                     /gene="xylB"
                     /locus_tag="ACHKUI_002757"
     CDS             complement(5666..7120)
                     /gene="xylB"
                     /locus_tag="ACHKUI_002757"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6598928.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGVLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFHRLYQQLLPLMA"
     gene            complement(7192..8514)
                     /gene="xylA"
                     /locus_tag="ACHKUI_002758"
     CDS             complement(7192..8514)
                     /gene="xylA"
                     /locus_tag="ACHKUI_002758"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6598929.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8880..9872
                     /gene="xylF"
                     /locus_tag="ACHKUI_002759"
     CDS             8880..9872
                     /gene="xylF"
                     /locus_tag="ACHKUI_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6598930.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9950..11491
                     /gene="xylG"
                     /locus_tag="ACHKUI_002760"
     CDS             9950..11491
                     /gene="xylG"
                     /locus_tag="ACHKUI_002760"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6598931.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11469..12650
                     /gene="xylH"
                     /locus_tag="ACHKUI_002761"
     CDS             11469..12650
                     /gene="xylH"
                     /locus_tag="ACHKUI_002761"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6598932.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12728..13906
                     /gene="xylR"
                     /locus_tag="ACHKUI_002762"
     CDS             12728..13906
                     /gene="xylR"
                     /locus_tag="ACHKUI_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6598933.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYVFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14014..14838)
                     /gene="bax"
                     /locus_tag="ACHKUI_002763"
     CDS             complement(14014..14838)
                     /gene="bax"
                     /locus_tag="ACHKUI_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6598934.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15158..17188
                     /gene="malS"
                     /locus_tag="ACHKUI_002764"
     CDS             15158..17188
                     /gene="malS"
                     /locus_tag="ACHKUI_002764"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6598935.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISLQNTVEPVASLVRECPKWDGLPLTLDVSTTFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDIVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17366..18619
                     /gene="avtA"
                     /locus_tag="ACHKUI_002765"
     CDS             17366..18619
                     /gene="avtA"
                     /locus_tag="ACHKUI_002765"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6598936.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18771..19244)
                     /gene="ysaA"
                     /locus_tag="ACHKUI_002766"
     CDS             complement(18771..19244)
                     /gene="ysaA"
                     /locus_tag="ACHKUI_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6598937.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19346..20194)
                     /gene="yiaJ"
                     /locus_tag="ACHKUI_002767"
     CDS             complement(19346..20194)
                     /gene="yiaJ"
                     /locus_tag="ACHKUI_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO6598938.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20395..21393
                     /gene="yiaK"
                     /locus_tag="ACHKUI_002768"
     CDS             20395..21393
                     /gene="yiaK"
                     /locus_tag="ACHKUI_002768"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO6598939.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDNETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITIVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21405..21869
                     /gene="yiaL"
                     /locus_tag="ACHKUI_002769"
     CDS             21405..21869
                     /gene="yiaL"
                     /locus_tag="ACHKUI_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6598940.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTIDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKLVVKVALAAL"
     gene            21892..22812
                     /locus_tag="ACHKUI_002770"
     CDS             21892..22812
                     /locus_tag="ACHKUI_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFO6598941.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            22926..23393
                     /gene="yiaM"
                     /locus_tag="ACHKUI_002771"
     CDS             22926..23393
                     /gene="yiaM"
                     /locus_tag="ACHKUI_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO6598942.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            23396..24673
                     /gene="yiaN"
                     /locus_tag="ACHKUI_002772"
     CDS             23396..24673
                     /gene="yiaN"
                     /locus_tag="ACHKUI_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO6598943.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            24686..25672
                     /gene="yiaO"
                     /locus_tag="ACHKUI_002773"
     CDS             24686..25672
                     /gene="yiaO"
                     /locus_tag="ACHKUI_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO6598944.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGAIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            25676..27172
                     /gene="lyxK"
                     /locus_tag="ACHKUI_002774"
     CDS             25676..27172
                     /gene="lyxK"
                     /locus_tag="ACHKUI_002774"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO6598945.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQKDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHKPERYAQIGCVMMTH
                     DYLRWCLTDVKGCEESNISESNLYNMGRGEYDPYLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            27169..27831
                     /gene="ulaD"
                     /locus_tag="ACHKUI_002775"
     CDS             27169..27831
                     /gene="ulaD"
                     /locus_tag="ACHKUI_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6598946.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAGDFHAQIDSIWGGK
                     RA"
     gene            27824..28684
                     /gene="sgbU"
                     /locus_tag="ACHKUI_002776"
     CDS             27824..28684
                     /gene="sgbU"
                     /locus_tag="ACHKUI_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO6598947.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28678..29373
                     /gene="araD"
                     /locus_tag="ACHKUI_002777"
     CDS             28678..29373
                     /gene="araD"
                     /locus_tag="ACHKUI_002777"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6598948.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(29420..30958)
                     /gene="aldB"
                     /locus_tag="ACHKUI_002778"
     CDS             complement(29420..30958)
                     /gene="aldB"
                     /locus_tag="ACHKUI_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6598949.1"
                     /translation="MTNNPPSARVQPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTIEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(31066..32361)
                     /locus_tag="ACHKUI_002779"
     CDS             complement(31066..32361)
                     /locus_tag="ACHKUI_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO6598950.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(32491..33642)
                     /gene="yiaY"
                     /locus_tag="ACHKUI_002780"
     CDS             complement(32491..33642)
                     /gene="yiaY"
                     /locus_tag="ACHKUI_002780"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6598951.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNILTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         8320 a         8663 c         8418 g         8300 t
ORIGIN      
        1 aagattatta aaggttgctg tattatttag cgataaaaaa agcctgccag atggcaggct
       61 atttaataac ggcattatta ctgtaacagc gaaatatccg caacgcgcag gaataattcg
      121 cgcagtttct ccagcatggt aagacggttg attcgcaatt ctttgtcatc aaccatgacc
      181 atcactttat cgaagaaagc atcgaccggt tcacgcagct cagccagttc gaccagcgca
      241 tcctggtaac gaccttccgc aaagtacggc tccagcttgt cacgtagcac cacaacctgc
      301 atcgccagtt taatttcttc cggctctttc agagtagagg cattcacgcg atcgctcagc
      361 acttcatcag atttcgccag gatgttagat acacgcttgt tcgccgccgc cagtgctgca
      421 gctgcttcca gggtacggaa gtgcgatacc gctttcatac gggcatcgaa atcagccgga
      481 cgagtcggac gacgcgccag taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga
      541 taccaggcgc ggaagcgacc gagcataaag tcgataacat catcaaccac gttggcatta
      601 gtcagcttat cgccatacag acgcactgct tcttcggtca gggtttgcag gtcaagattg
      661 aggttcttct caacgataat acgcagcacg ccaagtgcgg cacgacgcaa cgcaaacggg
      721 tctttgtcgc ctttcggatg ctggccgata ccgaagatac ccgccagagt atccatcttg
      781 tcagcaatcg ccagcgcaca ggctaccggg ttagacggca ggtcatcacc ggcaaagcgc
      841 ggctgatact gctcgttcag cgccactgcc acatcttccg cttcgccatc gtgacgcgcg
      901 tagtgcatcc ccataacgcc ctgggtgtcg gtgaactcga agaccatgtt ggtcatcagg
      961 tcgcacttgg acagcaggcc cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca
     1021 gcaatccagc cagccagcgc ctggatgcgg tcagttttgt cgcgcagtgt acccagctgt
     1081 tgctgaaaca acacggtttg caggcgcggc aggttatctt ccagacgttt tttacggtcg
     1141 gtgttgaaga agaactcggc atccgccaga cgcggacgaa cgactttctc gttaccggag
     1201 ataatctgct gcggatcttt cgattcgatg ttggcaacga agataaagtt cggcagcagt
     1261 ttgccgtcgt tcgcatacac cgggaagtat ttctggtcac ctttcatggt gtaaaccagc
     1321 gcttcagccg gcaccgcgag gaatttctct tcgaattttg cggtcagcac aaccggccat
     1381 tccaccagcg aagccacttc ttccagcaga ctttcgctta agtcagcgtt accgccaatc
     1441 ttacgcgctg cttcttcggc atcggcttta atcttcgctt tacgttcttc gtaatcggcg
     1501 atgactttcc cgcgctcacg cagaatttcc ggatactgat cggcgttatc gatggtgaat
     1561 tccggctcgc ccataaagcg gtggccgcga atcacgcgat cggactgaat gcccagaatg
     1621 gtagccggaa tgactttgtc gcccagcagc agggttacag tgtgaaccgg acgcacgaag
     1681 tgtacgtcag acgcgcccca acgcatcagt ttcgggatcg gcagtttcgc cagcgaagtc
     1741 gcaaccatat tcggcagcag tgcttcggtg ctttcgccct tcacatgggc gcgatacagc
     1801 agccattcgc ctttatcggt agtcagacgc tcagcctggt caacggtaat accgcaacca
     1861 cgcgcccaac cttctgccgc tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc
     1921 gccgggccgc gtttttcgat ttcacgatcc ggttgcgctt ccgccaggtt agcgactttc
     1981 agcgccagac gacgcggagc agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta
     2041 tccagctccg cagtaaaatt cgcagcaaag gactcagcca ggctgcgcag tgcttttggt
     2101 ggcagctctt cagtgccaat ttccaccaga aaagttttct cagacatagc cgcctcttac
     2161 ttatctttgt tgcacatcgg gaagccgagg gcttcacggg aagcgtagta tgcttctgcc
     2221 actgctttgg tcagggtgcg aatgcgcaga atatagcgct gacgctcggt gacggagatg
     2281 gctttacgcg catccagcag gttgaagctg tgggcggctt tcagaatacg ctcgtaggct
     2341 ggcagcggca gcggattttc cagcgccagc agttgctgcg cttctttctc gtactgctcg
     2401 aagcaggtga acaggaagtc cacatccgcg tattcgaagt tgtaagtgga ctgctccact
     2461 tcgttctgat ggaacacgtc gccgtaggtg gttttaccca gcgggccgtc gctccagacc
     2521 aggtcgtaaa cgctgtctac gccctgaatg tacatggcca gacgttccag accgtaggtg
     2581 atctcaccgg taaccggttt acattccaga ccaccaacct gctggaagta agtgaactgc
     2641 gtcacttcca tgccgtttag ccacacttcc cagcccagcc cccaggcacc cagcgtcggg
     2701 ttttcccagt tatcttccac gaaacggatg tcgtggatgg tcgggtccat gcccagctct
     2761 ttcagagaac cgaggtacag ctcctgaata ttgtccggcg atggcttaat gaccacctgg
     2821 aactgatagt agtgctgtaa acggttgggg ttttcgccgt agcgaccatc ggtcgggcga
     2881 cgagaaggct gaacataagc agccgccatc ggttctggcc ccagcgcgcg cagacaggtc
     2941 attgggtgag aggttcccgc gccgacttcc atgtccaatg gttgaacaat ggtgcagccc
     3001 tggcgagccc agtaatcctg taaggtcagg atcaagccct ggaaggtcct ggtatcaaac
     3061 ttttgcatat tatttcgtgc tggatacgtg tggatttaaa ggaagggatc agtatacccg
     3121 ctggatggaa gatatacagt acgaaacggg aaaaagcagg gcttaacgca tggaaagatg
     3181 caaaaaatgg ccatccgcgt caaaagaaca gacaaactgc tctttccgga aggtataacc
     3241 gcgcatttca tagcttccct ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc
     3301 ggtgttataa cgttttgccg cctgatcctt gcataacgct tccatattca acgaacgcag
     3361 aggatcaacc tttacccgtt gtgctttctg cacgggtggc ggtgtagaac aacccactag
     3421 cacaataagc gccattgccg gaaagaaagc gttcatcatc attaaattac cgtctggttt
     3481 gcaggtgagt cttattattt atatcggtat agtggctgat atcttacgaa tttccttaag
     3541 cctctataag ctgtagtgat aaaactcaga ttattccagt gcgccaactg aatgatttat
     3601 cggtcagact ttttttaacc agatacagag acacagatgc agcccaaaat ttactggatt
     3661 gataacctgc gagggatagc gtgtttaatg gtggtgatga ttcacaccac tacctggtat
     3721 gtgaccaatg ctcatagtgt tagccccatc acctgggata tcgccaatgt tctgaattct
     3781 gcctctcgtg tcagcgtgcc gctatttttc atgatttccg gctatctctt ttttggcgaa
     3841 cgcagcgccc agtcgcgcca tttcttgcgt atcggcttat gcctgttttt ttatagcgca
     3901 atcgcgctgc tctacattgc gctgtttacc tccatcaatg tggagttagc gctgaaaaac
     3961 ctgctgcaaa agccagtgtt ttaccattta tggtttttct tcgcgattgc agtgatttat
     4021 ctggtttcac cgctgattca ggtgaagaac gtcggcggaa aaatgttgct ggtgctaatg
     4081 gtggtgattg gtatcatcgc taacccaaat actgtgccgc agaaaatcga cggttttgaa
     4141 tggctgccaa ttaacttata tatcaatggc gatacttttt actacatcct ttatggcatg
     4201 ttgggccgcg ctatagggat gatggacaca cagcataaag cactgtcgtg ggtgagcgcc
     4261 gcactgtttg cgacgggggt ttttattatc tctcgcggga cattatatga attacagtgg
     4321 cgcggaaatt ttgccgatac ctggtatctt tactgtgggc cgatggtttt tatctgcgca
     4381 atcgcgttat tgactctggt taaaaacacg ctgtatacgc gtaccatttg cggacttggc
     4441 ttaatctccc gccattcgtt aggtatatac gggttccatg cattgattat ccatgcactg
     4501 cgcacccggg ggattgagct taaaaactgg ccaatactcg atattatttg gatcttttgc
     4561 gcgacgttgg cagcgagttt gttactttct atgctggtac aacgaatcga cagaaacaga
     4621 ttagtgagtt agtaaaagcc cggttacatt ggactaaccg ggcttatgtg aattattcgc
     4681 tgtattcttc ctgggtaacc tgtgtttctt ttggtgggtt tattccggca tcacgaatat
     4741 tcttctgcac cgctactgca ccaaacaagc ttaagaagaa agccagccca taaaagcctt
     4801 tttcgctgag taataacgtt gcattccata gacctaccag caacaacgca acagagataa
     4861 taaagaccgt caggcaagtc atataataaa tgggggtggt aggtatgcct tcatacttgt
     4921 cccgaacggt cttttgataa gacgccgcgg aaaacaaacc taataccagt acggcaaaat
     4981 aatacccttt ttcatttaac tgcatctctg cattccatag tcctaacaga taggtaacaa
     5041 taccgccaat aagggctacc catgaaacaa tactaaatgc cggagaatag gttgatattt
     5101 tgttgtccat cacagtattc ctttcattcc tgaatgtgta agaactttat gttgctcatg
     5161 ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg ggatagatca
     5221 ccatttcata agcattgata ggtgcgttta aaacaccgat aaacaagaga ccgatagtga
     5281 ttaacgctac caactgacag acgtggataa aaaaatcatc cagttgccca agtttttctg
     5341 cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga aaatagcctt
     5401 ttccacttaa caatgggcat gagatccata gtccaaccag ataaaccagc gccccaacaa
     5461 caaataatag ttttgcaaca gtctttgagg ttttcataac gatctccata tctaccagcg
     5521 atacattacg aataaccaac gaaagacaaa actgaaaaat gccattaaca aatgattttc
     5581 agaataaatt catactaaat attaattaat tactgagata tatagatgtg aattatcccc
     5641 cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc tgatagaggc
     5701 gatggaacgt ttctcgtcgt ggctgataag cggcataacg ctgcgcatct ggtagatgcg
     5761 actgttctaa cggtagttgc ggcaacaact caatgagcga tttctctgga ttcgccgcaa
     5821 tctgcgccag ccttgctgcg cccagtgctg gccccacatc gccacccgtg cggtaatcca
     5881 gttgctgacc gctgatgtcc gccagcattt gacgccagta ctcactacgc gccccgccac
     5941 caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc atgccatctg
     6001 ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg ccatgttgat
     6061 gagtcaaacc aaagaaaacc cctttcgcct ggggattatt gtgtggtgta cgctcgccgg
     6121 aaagataagg cagaaaccaa actggctcgg cactttcatc agcctgttga gctgcggtga
     6181 ttaaagcagg gacattgctc aggccggtta atttcgcggc ccaatccaga cacgaagccg
     6241 cactcagcat tacagacatt aaatgccaac gttgcggtaa cgcatggcaa aagctatgta
     6301 cggcgctttc tggcttgctt aagacccctt cgctgacagc aaaatagacc cccgacgtcc
     6361 ccagcgataa cattgcctga ttagcatcaa ccattcccac accaaccgca ccagctgcat
     6421 tgtcgccacc gcctgcgata actggcaccg ccgccatacc ccacgctttc gcaacttcag
     6481 gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg tcacgagata
     6541 agtggcaagc ctgcagcatg agatcgctcc agtcacgctt tgcgacatcc agccacatag
     6601 tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga cgcaagtaat
     6661 ctttcggtaa taatacttta tcgatttgac ggaatatctc cggctcatgc cgctgaaccc
     6721 atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc acccgcgatt
     6781 gcggaactcg cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg ttccacaaaa
     6841 tggcagggcg taacacccgt tgctgagcat ccagtaaggt tgcaccgtgc atctgtccgg
     6901 caatacctaa tgctttaacg tcttgcagag aatgctgaac gcccagagct ttcattgcgc
     6961 gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga tgcgggcgcg
     7021 aaacggtcag cttttccgtt tgcgcagcaa ccacctcacc ctgctcgttg agcaaaataa
     7081 cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt ccttaaaaaa
     7141 atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc gttatttgtc
     7201 gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct gatgcaccgg
     7261 agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt ggcctttcag
     7321 gatttgctgg cctaattcac tattccagcc ggaataacgc tgcgcgatgc gtttatccag
     7381 ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc aacgccatcg tatccatcgc
     7441 gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta ctttggcatc
     7501 gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca tcaccagcgc
     7561 attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg catcgccacg
     7621 gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt catgatggaa
     7681 agagtgacct gccagcgtcg cgtggttagc ttcaatattc agtttaatct ctttttccag
     7741 accaaactgt ttcaggaagc catagaccgt cgcagcatcg taatcatatt gatgtttggt
     7801 cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aaaccgattt tatgtttatg
     7861 ctcaaccacc atctgcataa agcggcccag ctgttcacgc tcctggcgca agtcggtatt
     7921 taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac cgcccaattt
     7981 atgggttgct tccatcgctg taacaacttg cgttgccgcc caactgaaga cttcaggatc
     8041 tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag cagttcccca
     8101 cagcagcttc acgccgctct cttcttgctt gcctgccagg acatcaacca tttgcgcaaa
     8161 attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat cgtggaagca
     8221 ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt tacgcttcgc
     8281 caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca caccaaacat
     8341 atccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca agtgctcttc
     8401 catacgcttg cccaacacca gttcgtcggg attgtagtga cggaatgcta acgggtttga
     8461 ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt gcatattgaa
     8521 ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg taatattggg cactcccttt
     8581 cagttgctca attatgttat ttcacactgc tattgagata attcacaagt gtgcgctcgc
     8641 tcgcaaaata aaatgggatg atgaaactgg gtaattcctc gaagagaaaa atgcaataag
     8701 tacaattgcg caacaaaagt aagatctcgg tcataaatca agaaataaac caaaaatcgt
     8761 aatcgaaaga taaaaatctg taattgtttt cccctgttta gttgctaaaa attggttacg
     8821 tttatcgcgg tgattgttac ttattaaagc tgtcctctaa ctacagaagg ccctacacca
     8881 tgaaaataaa gaacattcta ctcacccttt gcacctcact tctgcttacc aacgttgcgg
     8941 cacacgccaa agaggtcaaa ataggtatgg cgattgatga tctccgtctt gaacgctggc
     9001 aaaaagatcg agatattttt gtgaaaaaag cagaatctct cggcgcgaaa gtatttgtac
     9061 agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat tgaaaacatg attaaccggg
     9121 gtgtcgatgt tcttgtcatt attccttata acggtcaggt attaagtaac gttgtaaaag
     9181 aagccaaaca agaaggtatt aaagtattag cttacgaccg tatgattaac gatgcggata
     9241 tcgattttta tatttctttc gataatgaaa aagtcggtga actgcaggca aaagccctgg
     9301 tcgatattgt tccgcaaggt aattacttcc tgatgggcgg ctcgccggta gataacaacg
     9361 ccaagctgtt ccgcgccggg caaatgaaag tattaaaacc ttatgttgat tccggaaaaa
     9421 ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc ggaaaacgca ttgaaaatta
     9481 tggaaaacgc gctaaccgcc aataataaca aaattgatgc tgtagttgcc tcaaacgatg
     9541 ccaccgcagg aggggcaatt caggcattaa gcgcgcaagg tttatcaggg aaagtagcaa
     9601 tttccggcca ggatgcggat cttgcaggta ttaaacgtat tgctgccggt acacaaacta
     9661 tgacggtgta taaacctatt acattgttgg caaatactgc cgcagaaatt gccgttgagt
     9721 tgggcaatgg tcaggagcca aaagcggata ccacactgaa taatggcctg aaagatgttc
     9781 cctcccgcct gctgacaccg atcgatgtga ataaaaacaa catcaaagat acggtagtta
     9841 aagacggatt ccacaaagag agcgagctgt aagcgttacg ccccggcgcg gagcgggggc
     9901 gtgatttctc tccatgccgc gtgaattgat tggcttaggt ggagtcgtta tgccttatct
     9961 acttgaaatg aagaacatta ccaaaacctt cggcagtgtg aaggcgattg ataacgtcag
    10021 cttgcggttg aatgctggcg aaatcgtctc actctgtggg gaaaatgggt caggtaaatc
    10081 aacgctgatg aaagtgctgt gtggtattta tccccatggt tcctacgaag gcgaaattat
    10141 ttttgcggga gaagagattc aggcgagtca catccgcgat accgaacgca aaggtatcgc
    10201 cattattcac caggaattgg cgctggtgaa agaactgacc gtgctggaaa atatcttcct
    10261 gggtaacgaa ataacccata atggcattat ggattatgac ctgatgacgc tacgctgtca
    10321 gaagctgctc gcacaggtca gtttatccat ttcacctgat acccgcgttg gcgatttagg
    10381 acttgggcaa caacaactgg ttgaaattgc caaggcactt aataaacagg tgcgtttgtt
    10441 aattctcgat gagccgacag cctcattaac tgagcaggaa acgtcggttt tactggatat
    10501 tattcgcgat ctacaacagc acggtatcgc ctgtatttat atttcgcaca aactcaacga
    10561 agtcaaagcg atttccgata cgatttgcgt tattcgcgac ggtcagcaca ttggcacgcg
    10621 tgatgctgct ggaatgagtg aagacgatat tatcaccatg atggtcgggc gagagttaac
    10681 cgcgctttac cctaatgaac cacataccac cggagatgaa atattacgta ttgaacatct
    10741 gacggcatgg catccggtta atcgtcatat taaacgagtt aatgatgtct cgttttccct
    10801 gaaacgtggc gaaatactgg gtatcgccgg actcgttggt gccggacgta ccgagactat
    10861 tcagtgcctg tttggtgtgt ggcccggaca atgggaaggg aaaatttata ttgacggcaa
    10921 acaggtagat attcgtaact gtcagcaagc catcgcccag gggattgcga tggtacccga
    10981 agacagaaag cgcgacggca tcgttccggt aatggcggtt ggtaaaaata ttaccctcgc
    11041 cgcactcaaa aaatttaccg gtggcattag ccagcttgat gacgcggcag agcaaaaatg
    11101 tattctggaa tcaatccagc aactcaaagt taaaacgtcg tcccccgacc ttgctattgg
    11161 acgtttgagc ggcggcaatc agcaaaaagc gatcctcgct cgctgtctgt tacttaaccc
    11221 gcgcattctc attcttgatg aacccaccag gggtatcgat attggcgcga aatacgagat
    11281 ctacaaatta attaaccaac tcgtccagca gggtattgcc gttattgtca tctcttccga
    11341 attacctgaa gtgctcggcc ttagcgatcg tgtactggtg atgcatgaag ggaaactaaa
    11401 agccaacctg ataaatcata acctgactca ggagcaggtg atggaagccg cattgaggag
    11461 cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt agccgtaccg acatccggtg
    11521 gcttttccgg gctgaaatca ctgaatttgc aggtcttcgt gatgattgca gctatcatcg
    11581 caatcatgct gttctttacc tggaccaccg atggtgccta cttaagcgcc cgtaacgtct
    11641 ccaacctgtt acgccaaacc gcgattaccg gcatcctcgc ggtaggaatg gtgttcgtca
    11701 taatttctgc tgaaatcgac ctttccgtcg gctcaatgat ggggctgtta ggtggcgtcg
    11761 cggcgatttg tgacgtctgg ctaggctggc ctttgccact taccatcatt gtgacgttgg
    11821 ttctgggact gcttctcgga gcctggaacg gatggtgggt cgcgtaccgc aaagtccctt
    11881 catttattgt caccctcgcg ggcatgttgg catttcgtgg catactcatt ggcatcacca
    11941 acggcacgac tgtttccccc accagcgccg cgatgtcaca aattgggcaa agctatctac
    12001 ctgccagcac cggcttcatc attggcgcgc ttggcttaat ggcttttgtt ggctggcaat
    12061 ggcgcggaag aatgcgccgt caggctttgg gtttgcagtc tccggcctct accgcagtag
    12121 tcggtcgcca ggctttaacc gctatcatcg tattaggcgc aatctggcta ttgaatgatt
    12181 accgtggtgt tcccactcct gttctgctgc tgacgttgct gttactcggc ggaatgttta
    12241 tggcaacgcg gacggcattt ggacgacgca tttatgccat cggcggcaat ctggaagcgg
    12301 cacgtctctc cgggattaac gttgaacgca ccaaacttgc cgtgttcgct attaacggat
    12361 taatggtagc catcgccgga ttaatcctca gttctcgact cggcgctggt tcaccttctg
    12421 cgggaaatat cgccgaactg gacgcaattg cagcatgtgt gattggcggc accagtctgg
    12481 caggcggcgt cgggagcgtg gccggagcgg taatgggggc attcatcatg gcttcgctgg
    12541 ataacggcat gagtatgatg gatgtaccga ccttctggca gtatatcgtt aaaggtgcga
    12601 ttctgttgct ggcagtatgg atggactccg caaccaaacg ccgttcttga ttttgataaa
    12661 aattttctca aagccggtaa cgtattaccg gttttgagtt tttgcatgat tcagcaggaa
    12721 aagaaccatg tttactaaac gtcaccgcat cacattactg ttcaatgcca ataaagccta
    12781 tgaccggcag gtagtagaag gcgtagggga atatttacag gcgtcacaat cggaatggga
    12841 tattttcatt gaagaagatt tccgcgcccg cattgataaa atcaaggact ggttaggaga
    12901 tggcgtcatt gccgacttcg acgacaaaca aatcgagcaa gcgctggctg atgtcgacgt
    12961 ccccattgtt ggggttggtg gctcgtatca ccttgccgaa agttacccac ccgttcatta
    13021 cattgccacc gataactatg cgctggttga aagcgcattt ttgcatttaa aagagaaagg
    13081 cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc ggtaaacgtt gggccactga
    13141 acgcgaatat gtatttcgtc agcttgtcgc cgaagaaaag tatcgcggag tggtttatca
    13201 ggggttagaa accgcgccag agaactggca acacgcgcaa aatcggctgg cagactggct
    13261 acaaacgctg ccaccgcaaa ccgggattat tgccgttact gacgcccggg cgcggcatat
    13321 tctgcaagta tgtgaacatc tacacattcc cgtaccggaa aaattatgcg tgattggcat
    13381 cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc ctttcttcgg tcgctcaggg
    13441 cgcacggcaa atgggctatc aggcggcaaa actgttgcat cgtttattgg ataaagaaga
    13501 gatgccgttg caacggattt tggtcccgcc agtccgcgtg attgaacgac gttcaacgga
    13561 ttaccgttcg ttgactgatc ctgcggttat tcaggccatg cattacatcc gtaatcatgc
    13621 ctgcaaaggg attaaagtag atcaagtact cgatgcggtc ggaatctcac gctccaatct
    13681 tgagaagcgt tttaaagaag aggtgggtga aaccatccat gccatgattc atgccgagaa
    13741 gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg tcgatcaatg agatatcgca
    13801 aatgtgcggt tatccatcgc tgcaatattt ctactctgtt tttaaaaaag catatgacac
    13861 aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg ttgtagcgat gatgagaatt
    13921 atcggcgtcg catcaggtaa ctcagcacaa ccacctgata cgagcaaaag ccttatccgc
    13981 ctgacaagtc ggataaggct cgaggaaatg caattacata tgcgccgcga ttaaccgttg
    14041 gttatcctgg tacattgcga acaggtagtt gttataactc ttccctttgg tcgagtagcc
    14101 cttcagcttg tgaatcatcg ctgtggcagt cacttcctga tccgctttac gcagttgtgc
    14161 acgcgattta cggaacgaag agtaagccgg gtgcgtattg aggttagtga cataggcgct
    14221 caccgattct ttgacagaac taaactgtga gtaccctttc actttacccg gcgcattggt
    14281 acaacgtcct ttcatgcatt tcatgccgaa caggttgttg ttgttgcgcg ccagctttga
    14341 cgttccccaa ccgctttctg ctgcagccat cgtcgccacc atactggtag ggataatgtc
    14401 tacgcgttca agcaaggtat tccacgggat ttttcgcgta ttaccagacc acttcacctt
    14461 gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca ggtgaccatt ggccctgata
    14521 ctgttttgaa atcagccagt tacgttccgc agtaatggcc gcattttggc tggtaatgta
    14581 aggcattaca gtccggagaa acgccttttt ccttggtgtt ccggaagggt attttcgcaa
    14641 atcaggaagt gaactactct ttgcactatt gcgagaatac tcttgtttac tgcttacctg
    14701 tttattacta gctttagtta agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc
    14761 taacacctca ctcgaaaaca ccagagtgag taacataaga atcatcgccc catatcgtcg
    14821 tatgggagtc aaaatcatca ggtctcctgg tcggatttaa tcattccaac accttatatt
    14881 ttttacaaat ttgagagttg aatctcaaat catatcaaaa atagctgtca agagcacccc
    14941 aaggaatagt ccaaatctga aactatgtca cgagttaacg attcagattg gcgctaaatc
    15001 gcagaaaatg tggggtttat cgcaaaattc agccgttttt tgcgcgcgat cgctcaccct
    15061 tgcttctcat cctgtggact taccgctcag ggatgagttt tgtctggctt atcgctggca
    15121 aactgtctga aatcgcagca ataaggactc atccgccatg aaactcgccg cctgttttct
    15181 gacactcctt cctggcttcg ccgttgccgc cagctggact tctccggggt ttcccgcctt
    15241 tagcgaacag gggacgggaa catttgtcag ccacgcgcag ttgcccaaag gtacgcgtcc
    15301 actaatgcta aattttgacc aacagtgctg gcagcctgcg gatgcgataa aactcaatca
    15361 gatgctttcc ctgcaacctt gtagcaacac gccacctcaa tggcgattgt tcagggacgg
    15421 cgaatatacg ctgcaactag acacccgctc cggtacgcca acattgatga tttccctcca
    15481 gaacaccgtc gaaccggtag caagcctggt ccgtgaatgc ccgaaatggg atggattacc
    15541 gctcacactg gatgtcagca ccacttttgc ggagggagcc gccgtccggg attattacag
    15601 ccagcaaatt gcgatagtga agaacggtca aataacgtta caacccgctg ccaccagcaa
    15661 cggtttactc ctgctggaac gggcagaaac tgacacatcc gcccctttcg actggcataa
    15721 cgccacggtt tactttgtgc tgacagatcg tttcgaaaac ggcgatccca gtaatgacca
    15781 gagttacgga cgtcataaag acggtatggc ggaaattggc acttttcacg gcggcgattt
    15841 acgcggcctg accaacaaac tggattatct ccagcagttg ggcgttaatg ctttatggat
    15901 aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc ggtacaaaag gtgatttccc
    15961 gcattatgcc tatcatggtt attacactca ggactggacg aatcttgatg ccaatatggg
    16021 cagtgaagcc gatctacgga cgctggttga tagcgcgcat cagcgcggta ttcgtattct
    16081 ctttgatatc gtgatgaacc acactggcta tgccacgctg gcggatatgc aggagtatca
    16141 gtttggcgcg ttatatcttt ctggtgacga gctgaaaaaa acgctgggtg aacgctggag
    16201 cgactggaaa cctgccgccg ggcaaacctg gcatagcttt aacgattaca ttaatttcag
    16261 cgacaaaaca ggctgggata aatggtgggg aaaaaactgg atcagaacgg atatcggcga
    16321 ttacgacaat cctggattcg acgatctcac tatgtcgctg gcctttttgc cggatatcaa
    16381 aaccgaatca actaccgctt ctggtctgcc agtgttctat aaaaacaaaa cggataccca
    16441 cgctaaagtc atcgacggct ttacccctcg cgattactta acccactggt taagtcagtg
    16501 ggtccgcgac tatgggattg atggttttcg ggtcgatacc gccaaacatg ttgagttgcc
    16561 cgcctggcag caactgaaaa ccgaagccag cgctgcactt cgcgaatgga aaaaagctaa
    16621 ccccgacaaa gcattagatg acaaaccttt ctggatgacc ggtgaagcct ggggccacgg
    16681 cgtgatgcaa agtgactact atcgccacgg cttcgatgcg atgatcaatt tcgattatca
    16741 ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg gatacgacct ggcagcaaat
    16801 ggcggagaaa ttgcaggatt tcaacgtgtt gagctacctc tcgtcgcatg atacccgcct
    16861 gttccgtgaa gggggcgaca aagcagcaga gttattatta ttagcgccag gcgcggtaca
    16921 aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct accggttctg atccgctgca
    16981 aggtacgcgt tcggatatga actggcagga tgttagcggt aaatctgccg ccagcgtcgc
    17041 gcactggcag aaaatcagcc agttccgcgc ccgccatccc gcaattggcg cgggcaaaca
    17101 aacgacactt tcgctgaagc agggctacgg ctttgttcgt gagcatggcg acgataaagt
    17161 gctggtcatc tgggctgggc aacagtgatt tttccggctt cccgttcgtc agtacctcgg
    17221 gaagccgcca accaggataa aatgtcagcc ctaatcagca ttgcaggata aagcaccact
    17281 cactcttcaa cagaccgatt tgcaccccag caaatgtagc gttattgtta ccttccttgc
    17341 tacagagttc gacagatatc ccgctatgac attctccctt tttggtgaca aatttacccg
    17401 ccactccggc attacgctgc tgatggaaga tctgaacgac ggtttacgca cgcctggcgc
    17461 gattatgctc ggcggcggta atccggcgca gatccctgaa atgcaggact acttccagac
    17521 gctactgacc gacatgctgg aaagtggcaa agcgactgat gcactgtgca attatgacgg
    17581 tccacagggg aaaacggagc tactcacact gcttgccgga atgctgcgcg agaagttggg
    17641 ttgggatatc gaaccacaga atattgcact aacaaacggc agccagagcg cgtttttcta
    17701 cttatttaat ctgtttgccg gacgccgtgc cgatggtcgg gtcaaaaaag tgctgttccc
    17761 gctggcaccg gaatacattg gctacgctga cgccggactg gaagaagatc tgtttgtctc
    17821 tgcgcgtccg aatattgaac tgctgccgga aggccagttt aaataccacg tcgattttga
    17881 gcatctacat attggcgaag aaaccgggat gatttgcgtc tcccggccga cgaatccaac
    17941 aggcaatgtg attacagacg aagagttgct gaagcttgac gcgctggcga atcaacacgg
    18001 tattccgctg gtgattgata acgcttatgg cgtcccgttc ccgggtatca tcttcagcga
    18061 agcgcgcccg ctatggaatc cgaatatcgt gctgtgcatg agtctttcca agctggggct
    18121 acctggctcc cgctgcggca ttatcatcgc caatgaaaaa atcatcaccg ccatcaccaa
    18181 tatgaacggc attatcagcc tggcacctgg cggtattggt ccggcgatga tgtgtgaaat
    18241 gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca
    18301 gcgtgttcag gaaactatcg ctatcattcg ccgctattta ccggaagatc gctgcctgat
    18361 tcataaaccg gaaggagcca ttttcctctg gctatggttt aaggatttgc ccattacgac
    18421 cgagcagctc taccagcgcc tgaaagcacg cggcgtgctg atggtgccgg ggcacaactt
    18481 cttcccggga ctggataaac cgtggccgca tacgcatcaa tgtatgcgca tgaactacgt
    18541 accagagccg gagaaaattg aggcgggtgt gaagattctg gcggaagaga tagaaagagc
    18601 ctgggctgaa agccactaaa aattgcctga tgcgctacgc ttatcaggcc tacatgattt
    18661 ctgcaatata ttgaatttgg tagaatttgt aggccggata aggcgtttta gccgcatccg
    18721 gcataaacaa agcgcactat ccctgcgttg agaaacgccg gaaaaacgtt tcaaacaggc
    18781 tgctgccgta gccgatgccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc
    18841 gggcaggctt caacacaggc cggccccgtc gcccgatgcc agcacagatc gcacttaatc
    18901 gccctcgcct ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata
    18961 cagcttttac agccaatgca acgtgattgt tcaacgaaaa tatgcccatg ttcgcggctt
    19021 atcgcgtcaa cagggcacac atttgcgcac ggtgcatctt cacactgatg acaggctact
    19081 gccgtggtgt aagtatgatc tttaatgaca cgaatacggg aaataaactc gtctggtgac
    19141 aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg
    19201 caaccgatac atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac
    19261 taaaacagca gagtgccaga gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt
    19321 aaagttttgg cacttgtcca aagggttatg taatcgcgcc ctggtcgtcc ctgacagtaa
    19381 atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct
    19441 ctcccacctg tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc
    19501 catgaatatc aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt
    19561 ccatcgccgc tccactttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg
    19621 taatggtatt gcgggttaac ggctggatct catgctgatg gctttcccag tatgacttca
    19681 cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca
    19741 tatgctggcc tatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa
    19801 tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat agtttcacca gtggcgatgt
    19861 tcagagcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagat agcgccttct
    19921 gcccgacggc aataaatttg gtggtcaggc gataactccc tgcggcgggc gcggtggtca
    19981 catagccgca ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag
    20041 ccagctccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca
    20101 gcatcaaccc acgaaacaga ctctggcttc cggctgggcg ctctttttcc tgcgccatct
    20161 cgttctcttt ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa
    20221 agtgtgccgt agttcacgat ctcgacagat aatttataac cacttgattt ttaagttttt
    20281 tgaaattcat caatcagatt gctttgttaa aaagtgatcg atatatttga aatcaagttt
    20341 cgtatattga aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa
    20401 gtgacatttg agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac
    20461 aacgaaacgg ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat
    20521 tctcacggcg ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt
    20581 cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggacgcccag
    20641 cgttcgatcg gtaacctgac agcgaaaaag atgatggatc gcgccattga actggctgcc
    20701 gatcacggta ttggtctggt ggcactacgt aatgccaacc actggatgcg cggcggcagc
    20761 tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc
    20821 gtaatgccgc cgtggggcgc aaaagagtgt cgcatcggca ccaacccgct gatcgtcgcc
    20881 attccttcta ccccaatcac catagtcgat atgtcgatgt cgatgttctc ttacggcatg
    20941 ttagaagtta atcgcctggc tggtcgtcag ctcccggtag atggtggctt tgatgatgag
    21001 ggcaatttga ccaaagaacc tggcgttatc gagaaaaatc gccgcatttt gccgatgggc
    21061 tactggaaag gttcgggcat gtcgattgtg ctggatatga tcgctaccct cctttctgac
    21121 ggcgcatcgg ttgccgaagt caccgaggac aacagcgacg aatacggcat ttcacaaatt
    21181 tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc caaactgcaa
    21241 cgcatcatgg attacgttac cagtgccgag cgtgctgacg aaaatcaggc tattcgttta
    21301 cccggccacg aatttactac cctgctggca gaaaaccgcc gtaacggtat taccgttgat
    21361 gacagcgtgt gggcaaaaat ccaggcgtta taaggagata agccatgatt tttggacata
    21421 tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc
    21481 gcaccatcga cttcaacgcc ctggaaccgg gcgttgtcga aatcgacggc aagaatattt
    21541 atgcgcaaat tatcgattta accacacgcg aagcggtaga aaaccgtccg gaagtgcatc
    21601 gtcgatatat cgatattcag tttctggcgt ggggtgaaga gaaaatcggc attgctattg
    21661 atacgggaaa taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc
    21721 acgatagtga acatgaatca tttatagaaa tgattccggg cagctacgct attttcttcc
    21781 cgcaggatgt tcatcgcccg gcatgtaata aaaatgccac tacggcaata cgcaagctcg
    21841 tggttaaagt ggccctcgct gctttataaa aaatgattac tggagcacat aatgaaaacg
    21901 aataatgccg gttatattat cggcgcgtat ccctgtgcac cttcttttca ccaaagaagt
    21961 gaagaggaag agaaggattt ctggcgacaa ttatctgaga ctccagacat tttggggctg
    22021 gaacaacctt gcctggaaaa ttttcacccg ctgggcgatc aatggttact ccgtcatacg
    22081 ccagagagct ggaaatttgt cgtcaccgca gtgatggaaa ccatgcgtcg ccgcagcgaa
    22141 aacagcgggt ttggtctcgc ctccagtgat gaagaacagc gccaggcctg cgtggcgtac
    22201 tatcgccacc tgttcaataa aatcaactca ttgcaggcta ataaagtgct cgcactggag
    22261 ctccaggctg caccgttagc aacgaatcct aatgttatgc aagcgacgga cgcttttgcc
    22321 cgctcattga aagaaatcgc cagttgggac tggccgtgca agctggtgct ggaacactgc
    22381 gacgccatga catcttcttc cccccgaaaa ggctttttgc ctttagaaaa tgtgctggaa
    22441 gttattaccg attatgacat cagcatctgt atcaactggg cgcgctctgc cattgaaggg
    22501 cgcaatacaa cgctgccgct gacccacacc caaatggcga aacaagccgg aaaactcggc
    22561 gcgttaatgt tctctggtac gacgctaaac ggggcatacg gcgaatggca ggatttacac
    22621 gcaccgtttg caccattctg cgcagaaagc ctgatgacca cagatcatgt tcgcgagtta
    22681 tttaatgtag cagaatcgtc aacattgcat tttgctggca tcaagctact ggaaataaat
    22741 gcgacggctg atgtgcacca tcgcatcgaa atattacgta acggcattca ctctctgaat
    22801 gaatctcgtt aaatagttcc gcttatatat cactttgaat atctaattag ccgttttctg
    22861 ataattatta cgaatattaa caacattagc gcataaaaaa tattgcataa ccagaaggac
    22921 tcgttatgaa acggatactc gaaggcattc ttgcgatcat catcgcaata ctgtcttgta
    22981 ttatttttat taatatcatt cttcgatatg gattccacac aagtatatta tcgattgatg
    23041 aattatcccg acttttattt gtctggttaa cctttattgg cgcaatcgtt gcttatatgg
    23101 acaacagcca tgtgcaggtt acttttttag tcgaaaaact ctcaccggct aatcaacaac
    23161 gtgtttcgct gctaacccat acgctcattt tattgctgtg tctggggctg gcctggggcg
    23221 ctatagaaaa aacagcgcaa gactggagca acttatcgcc cattcttggc gtccccgttg
    23281 gcttaatgta cgccgccgct atccccacca gtctgattat cgcattgctg gaattgcgtc
    23341 acttataccg gcagttcaca aataccccca gccgaaacca acaaggagcc tgacgatgac
    23401 aatccttata tttattgtca gtttgttagg ggctatcgca attggcgttc ccatcgcctg
    23461 ggcattatta ctgtgcggca tttcattgat gttctggatg gatatctttg atgtgcaaat
    23521 tctggcacaa acgttggtaa atggcgcaga cagcttctcc ctgttagcca tccccttctt
    23581 tgtactggcg ggtgagatca tgaacgcggg cggcctgtcg caacgtatcg tcgacttgcc
    23641 aatgaaactg gtaggccaca ggccaggcgg tctgggttac gtgggcgtgc tggcagcgat
    23701 gatcatggca agcctgtccg gttctgcggt tgctgatacc gcagctgtcg cggcgctgct
    23761 ggtgccgatg atgcgccagg ccaactatcc ggtaaaccgc gccgccgggc ttatcggttc
    23821 cggcgggatt attgcggcca ttatcccgcc ttccatacca ctcattatct tcggcgtctc
    23881 cagcggactc tccatcagca aactgtttat ggcgggcatt gcgccaggca tcatgatggg
    23941 cgtcacgctg atggttacct ggtggtggca ggcaaaacga ctgaatttac cttgtcagcc
    24001 gaaagcctca ctgcgagaag tctggcaatc cttagtttcc ggtatttggg cgctcttttt
    24061 accgataatt attattggcg gtttccgttc ggggttattt acaccgaccg aagcaggcgc
    24121 ggttgccgcc ttctacgctc tgttcgtctc ggtggtggtc tatcgcgaga tgaccttctc
    24181 cacgctttat catgtgctga ttaacgcggc aaaaaccaca tccgtggtga tgttcctggt
    24241 ggcctctgct gcggtatctg catggcttat tacgatcgcc gaattaccca tgatggtatc
    24301 ggagctatta caaccgttgg ttgattcgcc tcgcttatta ttcatcgtca ttatgctcgc
    24361 tattatggtg atcagtacgg tcatggactt aacgcctacc gtattaattc ttacaccagt
    24421 attaatgcca ttagtgaaag aagcgggaat agatccggtc tatttcggca tcatgtttat
    24481 tatcaactgt tcaatatcac tgattacgcc tccagtcggt aatgttctta acgtggtatg
    24541 cggcgtggcg aaacttaaat ttgacgatgc ggtaaaaggc gttgcgcctt acgtcatggt
    24601 tttattcatg ttgttagcat tatttatttt tataccggag ctcattaccg ctcctcttaa
    24661 atggatgagt taaaaggaaa tcataatgaa attacgttct gtaacctacg ctttattaat
    24721 tgccggagtg gcgacgttca gcacatcttc tctggcggca caatcattac gtttcggtta
    24781 tgaaacctca caaaccgatt cacaacatat tgcggcgaaa aaatttaatg aattactgca
    24841 ggaaaaaacc aaaggcgaac tgaaattaaa actgttcccg gatagcaccc ttggtaatgc
    24901 gcaggcgatg atcagcggcg tgcgtggcgg cgctattgat atggagatgt ctggctctaa
    24961 caactttacg ggactctcgc ccgtcatcaa cctgctcgat gtcccgttcc tgttccgcga
    25021 taccgctcac gcgcataaaa cgctcgacgg caaagtcggt gatgacctga aagtctccct
    25081 tgaaggtaag gggctgaaag tactggccta ctgggaaaac ggctggcgcg atgtcaccaa
    25141 ctcgcgtgca ccggtcaaaa cgcctgccga cctgaaaggg ttgaaaatcc gcaccaacaa
    25201 cagcccgatg aatatcgctg catttaaagt ttttggcgct aacccgatcc cgatgccgtt
    25261 tgccgaagtc tataccgggc tggaaacccg cactatcgac gctcaggaac acccgatcaa
    25321 cgtcgtctgg tcagcaaaat tttatgaagt gcagaagtac ctttctctga cccatcacgc
    25381 ctattcgccg cttctggtgg tgatcaacaa agcgaagttt gatggcttaa ccccggagtt
    25441 ccagcaagca ctgatctcat ctgcacagga agcgggtagc tatcagcgca aactggtcgc
    25501 cgaagatcag caaaaaatca tcgacggtat gaaagaagcg ggcgtggaag tcatcaccga
    25561 tctcgaccgc aaagccttta gcgatgcgct gggtacccag gtccgtgaca tgtttgttaa
    25621 agatgttccg cagggtgctg atctgctgaa agccgtggat gaggtgcaat aaaccatgac
    25681 gcaatactgg ctggggttag attgtggcgg tagctggctg aaagccgggc tgtatgaccg
    25741 agaaggccgg gaggcgggcg tgcagcgcct gccgctgtgc gcattaagtc cacagccagg
    25801 ctgggcagag cgcgacatgg cagaactgtg gcaatgttgc actgctgtca ttcgcgccct
    25861 gcttacccat tctggcgtca gcggggaaca aattgtcggt atcggcatct ccgcgcaggg
    25921 aaagggctta tttctacttg ataaaaacga caaaccgctc ggtaacgcca ttttgtcctc
    25981 ggatcgccgg gcgatggaaa tcgttcgtcg ctggcagaaa gatggcatcc cggaaaaact
    26041 ctatccgctg acccgacaaa ccttatggac cgggcatccg gtgtcgctgt tacgctggct
    26101 gaaagagcac aaaccagaac gctacgcgca aattggctgc gtgatgatga cgcacgacta
    26161 cctgcgctgg tgtttaaccg acgtcaaagg ttgcgaagag agcaatattt ctgagtccaa
    26221 tctctacaac atgggtcgcg gggaatatga cccgtacctt accgactggc tggggatcgc
    26281 tgaaatcaat cacgccctgc cgcctgttgt cggatctgcc gaaatctgcg gggaaatcac
    26341 cgctcagaca gccgtactga ccggtctgaa agcgggtacc cccgttgtcg gcggcctgtt
    26401 tgatgtggtt tccaccgcac tctgcgccgg gctcgaagac gaattcaccc tcaatgcggt
    26461 gatggggacc tgggcggtga ccagcggcat aacccacggt ttacgtgacg gtgaagcgca
    26521 tccgtatgtc tatggtcgct acgttaacga tggtcaattt atcgttcacg aagccagccc
    26581 cacctcttcc ggcaatctcg aatggtttac cgcacagtgg ggagaaatct catttgctga
    26641 gattaatcag gccgttgcca gtttgccgaa agccggtggc gatctctttt tcctgccgtt
    26701 cctgtacggc agcaacgccg gactcgaaat gaccagcggt ttctacggga tgcaggccat
    26761 tcacacccgt gcacacctct tacaggccat ctatgaaggc gtggtgttca gccatatgac
    26821 tcatctcaac cggatgcgcg aacgttttac tgatgtgcac accctgcgcg tcactggcgg
    26881 cccggcgcac tccgatgtct ggatgcaaat gctggcggac gtcagcggtc tgcgtatcga
    26941 gctgccgcag gtggaagaaa ccggctgctt tggtgcggca cttgccgccc gcgtcggtac
    27001 cggggtttat cgcgatttca gcgaagccca acgtgactta cagcacccgg tacgcaccct
    27061 gctgccggat atggccgcgc atcagcttta ccaacaaaaa taccaacgct atcaacatct
    27121 cattgccgca cttcagggtt atcacgcccg catcaaggag cacacattat gagtcgacca
    27181 cttctgcaac tggccctcga ccactcatca cttgaagccg cgcagcgcga cgtgacgcgg
    27241 ttaaaagaca gcgtcgatat tgtcgaagca ggcaccattc tctgtttaaa cgaagggctt
    27301 ggcgccgtga aagccttgcg cgaacagtgc cctgacaaaa tcatcgttgc tgactggaag
    27361 gtcgccgacg cgggtgaaac gctcgcgcaa caggcatttg gcgcaggcgc taactggatg
    27421 accatcatct gcgccgcgcc gctcgccacg gtagaaaaag gccacgcgat ggcacagaac
    27481 tgcggtggtg aaattcagat cgagctattc ggtaactgga cgctggacga tgcccgtgac
    27541 tggcatcgta ttggcgtgcg gcaggccatt tatcatcgtg gtcgtgacgc gcaggccagc
    27601 gggcaacagt ggggcgaggc cgatctggca cgcatgaagg cgctttccga tatcggcctt
    27661 gagctttcca ttaccggcgg gattacccct gctgacctgc cgctgtttaa agagatccgc
    27721 gtgaaagcgt ttattgccgg acgggcgctg gcaggcgcag caaatccggc gcaagtggct
    27781 ggcgatttcc atgcgcaaat cgactccatc tggggaggca agcgtgcgta atcatccgtt
    27841 aggcatttat gaaaaagcgc tggcgaaaga tctctcctgg ccggagcggc tggtgctggc
    27901 aaaaagctgt ggttttgatt ttgtcgaaat gtcggtggat gaaaccgacg aacggttgtc
    27961 acgtcttgac tggagcgccg cacaacgtac ttcgctggtt accgcgatga tcgaaacggg
    28021 tgttgctatt ccgtcaatgt gcctgtccgc acatcgtcgt tttccttttg gtagccggga
    28081 cgacgccgtg cgccagcggg cgcgggagat tatgagtaaa gcgattcgcc tggcgcgcga
    28141 tctcggcatt cgtactattc aactggcagg ttacgacgtc tattacgaag accacgacga
    28201 aggcacccag caacgttttg ctgaagggct ggcgtgggcg gtcgaacagg cggcggcatc
    28261 acaagtcatg ctggcagtgg agattatgga caccgcgttt atgaactcca tcagcaaatg
    28321 gaaaaaatgg gacgagatgc tcgcctcgcc gtggttcacc gtctacccgg acgtcggcaa
    28381 cctcagcgcc tggggcaatg atgttcccgc cgaactgaaa ctgggcatcg accgtatcgc
    28441 cgccatccac ctgaaagaca ctcaacccgt taccgaacat agccccggac agttccgcga
    28501 tgtgccgttt ggcgaaggct gtgtcgattt tgttggcatc ttcaaaacgc tgcatgagct
    28561 gaactatcgc ggatcatttc tgattgaaat gtggaccgaa aaagccaaag agccggtgct
    28621 ggagattatt caggcgcggc gctggattga agcgcgtatg caggaggccg gatttatatg
    28681 ttagagcaac tgaaagctga cgtgctggcg gcaaacctgg cgcttcccgt tcatcatctg
    28741 gtgacgttca cctggggcaa tgtcagcgcg atagacgaaa cgcggcaatg gatggtgatt
    28801 aagccttccg gcgttgagta tgaggtaatg accgccgacg atatggtggt ggttgagata
    28861 gccagcggca aggtggtgga aggcagcaaa aagccctctt ccgacacgcc aacgcatctg
    28921 gcgctttacc gtcgctttgc ggagattggc ggcatagtgc atacccactc gcgccacgcc
    28981 accatctggt cgcaggccgg acttgatctc cccgcctggg gcactactca cgccgattat
    29041 ttttacggag ccatcccctg cacgcgcctg atgaccgcag aagagatcaa cggcgagtat
    29101 gaataccaga ccggcgaagt gatcattaaa accttcgaag agcgtgggtt gaacccggca
    29161 caaatcccgg cggtactggt gcattctcac ggcccgttcg cctggggaaa aaatgccgcc
    29221 gatgcggtgc ataacgccgt ggtactggaa gagtgcgcct atatgggtct gttctcgcgc
    29281 cagcttgcgc cacagctccc tgcgatgcaa aacgaactgc tggataagca ctacctgcgt
    29341 aagcatgggg ccaatgccta ttacgggcag tagccatgcc tgagagtgcc cggcaataca
    29401 cgccgggcag cctttcacat cagaacagcc ccaacggttt atccgagtag ctcaccagca
    29461 ggcacttggt ttgctggtaa tgctcaagca tcattttgtg ggtttcgcgg ccaatgcctg
    29521 attgtttgta gccgccaaac gccgcatgtg ccgggtaagc gtgataacag ttggtccaca
    29581 cgcgcccagc ctgaatgccg cgccccatct tataggccag attaccgttg cggctccaga
    29641 cgcccgcgcc caggccatat tgcgtatcgt tcgccagctc cagcgcttct tctatcgttt
    29701 tgaaggtggt taccgccagc accgggccaa aaatctcctc ctggaacacg cgcatattgt
    29761 tctgaccaaa cagaatcgtc ggttcgaggt agtagccgtc tttcagttcg ccttccagca
    29821 gcttgcgtcg cccgccggtg agcacatcag cgccctcttt tttaccgata tcaatgtagt
    29881 tgaggatggt ttccagttgc ccgtgagaaa cctgcgcgcc catttgcgtc acgctgtcga
    29941 gcgggttacc gctgcgaatg ctttcgacac ggcggatagc gcgttccata aagcgttcgt
    30001 agatagactc ctgcactaaa gcacggcttg gacaggtgca aacttcgccc tggttaaagg
    30061 caaacagtgc aaagccttcc agcgctttat caaaaaaggc atcttcttca tccatcacat
    30121 cagcaaagaa gatatttggc gatttgccgc ccagctccag cgtcaccgga ataatgtttt
    30181 gcgtggcgta ttgcataatt tgttggccca cttccgttga gccagtaaac gccactttgg
    30241 cgatgcgttt cgaggtcgcc agatattcgc caatttcccc acctgcgccg ttgaccacgt
    30301 tcaccacccc tggcggcagt aaatcaccga cgatttccat tagcagcagt acagaaagcg
    30361 gagtaagacg tgcgggtttc agcaccacac agttgcccgc cgccagcgcg ggagccattt
    30421 tccagctcgc catcagcagc gggaagttcc acgggataat ctgccccacc acgcctaacg
    30481 gttcatggaa atgataggcc acggtttcgc tatccacttc actgatccca ccttcctgcg
    30541 cccgaataca cgaggcgaaa tagcgaaaat gatcaatcgc cagcggcacg tcggcagcac
    30601 tggtttcgcg aatgggtttg ccgttatccc aggtttctgc cgtcgctaaa agttcaaggt
    30661 tttgttccat tcgatcggca atcttaaaca gaatcgccgc gcgatcctgc accgaggtgt
    30721 gcgcccattt atctttcact ttatgcgcag catccagcgc cagatcgatg tctcgtttgc
    30781 ccgaagacgc cacttcgcac agtagctgcc cggtcaccgg cgtcaggttc tggtaatact
    30841 cgccatcggc aggcgctacc cattcgccac cgataaagtt gtcgtagcgg gcttttaatt
    30901 tgagggggaa accatactcg ccgggctgaa cacgtgctga agggggatta ttggtcatga
    30961 tcgtctcctt gcggtgtgag gtataacaag ggtagacgta actggcgaaa tcttcgccag
    31021 tcggtgacag ctttacgacg gctatcacga atttacgggc aagggttaca gcggataatg
    31081 cgcgaagtac acggcttgct ggtcattaat tttcttctct tgcgcaaacc cttctttaac
    31141 aattgtccgt aaatcagctc gagccgtatt atcagagacg cccaagctcg cgctaatttc
    31201 agcaactgtg tactctttcc ccggattggc gagcatcaca ttcaggagcg ttacctggcg
    31261 cggattcagg cgtttaagca caccagagct aaacagcaca ctatcgagtt ttgcgcttcg
    31321 ttgtgtaatt tcatcgatat gctgctgcca gttttgcaga gcacgtttaa tgacacccgc
    31381 ctgatattca aggaaataag tcagatccat accatctgat tcagtgtact ggtagctggc
    31441 ggcatatttg accggtgcag catgaagcaa acggctgatg gaaatatatt taaagacatc
    31501 gtagcccgat ttcagcatat accagtaaaa cagcgcccga ctggtgcgcc cgttgccatc
    31561 ccgaaacgga tgttcatggg ccagcataaa atgcagaatg caggcacgga ccaacggatg
    31621 aatataccct tcatggctgt ttaaccactg gcaaactttt tccagccgtt ccggtaacaa
    31681 tgccgcagcg ggcggttgat gcactatatt gccatcatag tcggcgatca caacatcatc
    31741 tgtttcacga aattctcccg gacgatattt cgcgtcattg attccaccca taccaatggc
    31801 atgtaactgg cgaataagcg caggtgtcag cggttctgcg agtaagtgcg gaatttccgc
    31861 catcagccgg gcatttcccg caatcatatg ctcgtcttca gtgcgtggcg accgccctgt
    31921 ttccagcatt tttctcgcca ccagcgttgt agtattcgca ccttccagct gggagctggt
    31981 gatcggctct tccaggcgta actggcttaa ctcggctccg gccccgcgta accgttcagt
    32041 ctgatctttc agcgccagcg cggtagcacg tttgtcgaca agctcgcacg cttctgcaat
    32101 caccggggta atgttgaatc cggcctgttc acccgcttca ttgcaataat taattcgttg
    32161 gatcgccgcg ttgcgcgcca gacgggtaag cgtccacgcc acatcgacgt tttctccttt
    32221 tttgatgcgg tactgcaact catcaaaagg cagataacgt cctttttcat cggtaacccg
    32281 gttaaagcgc aggtgggcca ggagttcctc acccgataag tgtgataaag gaataagtcc
    32341 gggaggtgat ttgatagcca tgtgcttatc tcaaaaaagc gcaatattga gatagtgtat
    32401 agcaggaaag agaaactatc tcaaaaaaag caagttttga gatagttgaa aaagcggctg
    32461 acaactcgcc agccgttgtg gaaatgatga ttacatcgct gcgcgataaa tcgccacaat
    32521 ttcttcgtga gttgcctgga tcgggttagt aaagccgcag gcatctttca gggcattagt
    32581 cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg ccgggatatc
    32641 cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt ccgcgtcgtt
    32701 tttacctgtc acgttcacgc ccattgcagc ggcacaatca cgcagacgtg cagcggcgac
    32761 tttgctgttg aatacctgaa catgcggcag caaaacggcg ttacatacac cgtgtggcag
    32821 gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag aagcattatt
    32881 gaacgccata ccggcgagga actgggcata agccattgct tcacgcgctt tcgcattact
    32941 gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt tcagtgcaca
    33001 agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt gcgttaaggc
    33061 atccataccc gttgcggcgg tcagtgactt cggcatacca atcatcagag aggagtcatt
    33121 gacagaaagc aacggagtga catgtttatc cacaatcgcc attttgatgt gacgcgcttc
    33181 gtcagtgatg atgcagaaac gggtcatttc agaggccgta cccgccgtgg tgttgatggc
    33241 gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcataatcgc ggatgtcacc
    33301 gccgttggct gcaaccagcg caataccttt tgcgcagtcg tgtggagaac caccgcctaa
    33361 agaaatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcag cgacgttttc
    33421 cgtggtgggg ttaggttggg tgccatcata aataacgcta aaaatattgc gttcttccag
    33481 tgctttttgc acatcacccg ccatacccaa tttcgttaat atgttgtcgg tgacaattaa
    33541 ggtacgggta aatccataat ctgccatcat attcattgca tcattcaatg aatcagcacc
    33601 aataacattc acggaaggaa taaaaaaggt tgaagctgcc ataacacact cctgaaagtg
    33661 taaaaaaggg cgggcagctt actgaggatt ttcatttgaa a
//
LOCUS       DAZFRE010000052        33644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFRE010000052 DAZFRE010000000
VERSION     DAZFRE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.052"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..190)
                     /gene="safA"
                     /locus_tag="ACHKUI_002781"
     CDS             complement(<1..190)
                     /gene="safA"
                     /locus_tag="ACHKUI_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6598952.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAINQLG
                     ISEMGRIARDLTHFIINSL"
     gene            complement(438..2717)
                     /gene="ydeP"
                     /locus_tag="ACHKUI_002782"
     CDS             complement(438..2717)
                     /gene="ydeP"
                     /locus_tag="ACHKUI_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6598953.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLCERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(3056..3970)
                     /gene="ydeQ"
                     /locus_tag="ACHKUI_002783"
     CDS             complement(3056..3970)
                     /gene="ydeQ"
                     /locus_tag="ACHKUI_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598954.1"
                     /translation="MGKTFSIKVLFGIYLLLIAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
     gene            complement(4030..4533)
                     /gene="ydeR"
                     /locus_tag="ACHKUI_002784"
     CDS             complement(4030..4533)
                     /gene="ydeR"
                     /locus_tag="ACHKUI_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598955.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSTVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(4546..5076)
                     /gene="ydeS"
                     /locus_tag="ACHKUI_002785"
     CDS             complement(4546..5076)
                     /gene="ydeS"
                     /locus_tag="ACHKUI_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6598956.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTEVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPDQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            complement(5090..5824)
                     /locus_tag="ACHKUI_002786"
                     /pseudo
     CDS             complement(5090..5824)
                     /locus_tag="ACHKUI_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(5835..7063)
                     /locus_tag="ACHKUI_002787"
     CDS             complement(join(5835..6748,6748..7063))
                     /locus_tag="ACHKUI_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6598957.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            complement(7155..9077)
                     /locus_tag="ACHKUI_002788"
                     /pseudo
     CDS             complement(7155..9077)
                     /locus_tag="ACHKUI_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(9119..9829)
                     /locus_tag="ACHKUI_002789"
     CDS             complement(9119..9829)
                     /locus_tag="ACHKUI_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6598958.1"
                     /translation="MQTTRTPYSVSFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSKFTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(10191..10754)
                     /gene="fimA"
                     /locus_tag="ACHKUI_002790"
     CDS             complement(10191..10754)
                     /gene="fimA"
                     /locus_tag="ACHKUI_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6598959.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(11187..11453)
                     /locus_tag="ACHKUI_002791"
                     /pseudo
     CDS             complement(11187..11453)
                     /locus_tag="ACHKUI_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(11724..12125)
                     /gene="hipA"
                     /locus_tag="ACHKUI_002792"
                     /pseudo
     CDS             complement(11724..12125)
                     /gene="hipA"
                     /locus_tag="ACHKUI_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            complement(12190..13104)
                     /gene="yneE"
                     /locus_tag="ACHKUI_002793"
     CDS             complement(12190..13104)
                     /gene="yneE"
                     /locus_tag="ACHKUI_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6598960.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAQYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(13301..14752)
                     /gene="uxaB"
                     /locus_tag="ACHKUI_002794"
     CDS             complement(13301..14752)
                     /gene="uxaB"
                     /locus_tag="ACHKUI_002794"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6598961.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(14979..16397)
                     /locus_tag="ACHKUI_002795"
     CDS             complement(14979..16397)
                     /locus_tag="ACHKUI_002795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6598962.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(16536..16895)
                     /gene="yneG"
                     /locus_tag="ACHKUI_002796"
     CDS             complement(16536..16895)
                     /gene="yneG"
                     /locus_tag="ACHKUI_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6598963.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(16895..17821)
                     /gene="glsB"
                     /locus_tag="ACHKUI_002797"
     CDS             complement(16895..17821)
                     /gene="glsB"
                     /locus_tag="ACHKUI_002797"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6598964.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(17885..19273)
                     /gene="sad"
                     /locus_tag="ACHKUI_002798"
     CDS             complement(17885..19273)
                     /gene="sad"
                     /locus_tag="ACHKUI_002798"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6598965.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKTAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITTAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            19374..20255
                     /gene="yneJ"
                     /locus_tag="ACHKUI_002799"
     CDS             19374..20255
                     /gene="yneJ"
                     /locus_tag="ACHKUI_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6598966.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            20333..20452
                     /gene="yneK"
                     /locus_tag="ACHKUI_002800"
     CDS             20333..20452
                     /gene="yneK"
                     /locus_tag="ACHKUI_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO6598967.1"
                     /translation="MVTPVSISNSISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            20594..21784
                     /gene="ydeA"
                     /locus_tag="ACHKUI_002801"
     CDS             20594..21784
                     /gene="ydeA"
                     /locus_tag="ACHKUI_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6598968.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(21809..22474)
                     /gene="marC"
                     /locus_tag="ACHKUI_002802"
     CDS             complement(21809..22474)
                     /gene="marC"
                     /locus_tag="ACHKUI_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6598969.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            22686..23120
                     /gene="marR"
                     /locus_tag="ACHKUI_002803"
     CDS             22686..23120
                     /gene="marR"
                     /locus_tag="ACHKUI_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6598970.1"
                     /translation="MKNTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            23141..23524
                     /gene="marA"
                     /locus_tag="ACHKUI_002804"
     CDS             23141..23524
                     /gene="marA"
                     /locus_tag="ACHKUI_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6598971.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            23556..23774
                     /gene="marB"
                     /locus_tag="ACHKUI_002805"
     CDS             23556..23774
                     /gene="marB"
                     /locus_tag="ACHKUI_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6598972.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(23831..25270)
                     /locus_tag="ACHKUI_002806"
     CDS             complement(23831..25270)
                     /locus_tag="ACHKUI_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6598973.1"
                     /translation="MSGFKEDFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIMYQAVHYEL
                     VASALAVQTGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEIKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(25294..26967)
                     /locus_tag="ACHKUI_002807"
     CDS             complement(25294..26967)
                     /locus_tag="ACHKUI_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6598974.1"
                     /translation="MNIKTLNVSLLSISIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKFRLSTLQKSITENKYQSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQIAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGRTANAVVTYDGNVGQQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAREADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(27022..27333)
                     /locus_tag="ACHKUI_002808"
     CDS             complement(27022..27333)
                     /locus_tag="ACHKUI_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO6598975.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATKLMASS
                     ESACLQAHKIQTALISQDEGCGKIEVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(27361..28683)
                     /locus_tag="ACHKUI_002809"
     CDS             complement(27361..28683)
                     /locus_tag="ACHKUI_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6598976.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNVAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(28798..29109)
                     /locus_tag="ACHKUI_002810"
     CDS             complement(28798..29109)
                     /locus_tag="ACHKUI_002810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6598977.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHATAISLEVNISALAITEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            29308..30006
                     /locus_tag="ACHKUI_002811"
     CDS             29308..30006
                     /locus_tag="ACHKUI_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6598978.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(30051..30950)
                     /gene="eamA"
                     /locus_tag="ACHKUI_002812"
     CDS             complement(30051..30950)
                     /gene="eamA"
                     /locus_tag="ACHKUI_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6598979.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            31068..32222
                     /gene="ydeE"
                     /locus_tag="ACHKUI_002813"
     CDS             31068..32222
                     /gene="ydeE"
                     /locus_tag="ACHKUI_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6598980.1"
                     /translation="MLRIFAMLTIGRGATLPFMTIYLSRQYSLSVDLIGYAMTIALTI
                     GVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVF
                     ATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICS
                     AFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCI
                     SQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIG
                     LVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLG
                     AAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQPALC"
     gene            32349..32444
                     /gene="mgtS"
                     /locus_tag="ACHKUI_002814"
     CDS             32349..32444
                     /gene="mgtS"
                     /locus_tag="ACHKUI_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6598981.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            32444..32548
                     /gene="mgtT"
                     /locus_tag="ACHKUI_002815"
     CDS             32444..32548
                     /gene="mgtT"
                     /locus_tag="ACHKUI_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6598982.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(32663..33553)
                     /gene="dgcZ"
                     /locus_tag="ACHKUI_002816"
     CDS             complement(32663..33553)
                     /gene="dgcZ"
                     /locus_tag="ACHKUI_002816"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6598983.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         9061 a         8123 c         7444 g         9016 t
ORIGIN      
        1 gcaaactatt gataatgaaa tgtgtaagat cccttgcaat gcgacccatt tctgaaatac
       61 ccagctgatt aattgccgaa aaaatggcta tcaatgtaag ggtcagtaat aagacaacca
      121 caattacaga catgatccct ttatagttgt ctctttgcgt gattttattt ttcactatgg
      181 tcgcatgcat ttcaaatgtg tttattaaac gaatgacgtt aagcatcgtt aatcagtatg
      241 cttaatataa caggctggag aatagaggaa ataggacggg tacttcagta ctgagcggag
      301 tttcttacag ctgtaggcag aagtttatat cttatagtgt gtggtttaat ttaatccatg
      361 tgagcaaatt gcgcgataat agtcaatgag gaattcttct gctgtgcgga tgacagcaga
      421 agaaatgagg aggcgaatta atttgatggt tctaattcaa ccggaatact tttatagcca
      481 ggaatgccac ttaatggatc gtggttatca agtgttagca tgtgattcga ttctggaaaa
      541 taggtcacca gtgagcggtc agccataggg taaatgacca cttttaatct atccatgcgg
      601 cgtgagctac gtttaccgtc tggcgtgagt gcaataagat taactctttc gccgtttttt
      661 acacggcaaa ttttagcttg tttagcactc ataaagacca catctcgttg accgaatacc
      721 cctcgatagc gatcatccat accataaatc gtcgtgttgt actgatcgtg actgcgtact
      781 gtcgccatga ccagcttact gttaaacgct gaagagggat cttctaacag ccctttactg
      841 gtaatgaaat tagccttacc tgacgacgtc atccagcgcc tttcagctgc tgcgtttatc
      901 aggtgaaaac caccaggatg acggatgcgc tggttatagt cggcaaactc tggaagcaca
      961 gcttcaatgt cattgcgaat gcgatcataa tcttccacca gatactccca ggctaccacg
     1021 ctctggggta gtgctgcttg cgcgattccc gcgaccactg cacactctga tttcagcatt
     1081 acaccggcgg gttttaacac accacgcgag gcatgaatca tcgacattga atcttcaacg
     1141 gttaccgcct gcgcaccgct tttttgcatg tcaatctcgc tacgtcccag aaccggcaga
     1201 atatagctat gccgtgcggt cagcagatga gagcggttaa gcttagtggc tacgtgtacc
     1261 gccagatcta attgcgttaa cggtacagcg ctcgcttccc gatctggcat tgccagtgca
     1321 aagttgcccc ccatgcagat caatgctcga gcttgccccg tacatatagc ttgcatgctg
     1381 gcgattgcag catgtccagg tgcatgaggt ggggtgaagc catagcgctc acacaggcga
     1441 tccagaaact ctgcagacgg tttctcggtg ataccgacgg ttcggtcgcc ctgtacatta
     1501 gagtgacctc gtagtgggca gatacccgca ccaggcttgc caatgttacc tttcatcaac
     1561 agcagattga ccagttgctg tacgttctgg gtaccatgtt cgtgctgagt gatccccatt
     1621 ccgtaacaga taatagttcg ttcggcagcg gcatatgcat cagcaagttc ggcgatttgt
     1681 gtctgactta gtccagaaat acgttcgata tctttccact cggaattgag cacgtcacgg
     1741 cgtagctcgt caaagccgac ggtatgcgtt tgaataaatt cgtcatccag caatgagggc
     1801 cgacctgcgg cgcttgcagc atcatcgcgc tcaattaaca ggcgcatcat ccccttgagc
     1861 aatgccatat cgccaccaat gcgcacgtta tagtaggcac tggccaactg agtctcagag
     1921 ttcgtcagca tttcaaacgg gttttgcggt gcggtaaatc gctccaggcc acgttcctgt
     1981 agaggattga tggcgatcat tttcgctccc cgtttcacta aagcgcgcaa cgaagtcagc
     2041 atgcgagggt ggtttgtacc agggttatgc ccaatgcaaa tgactaaatc gcatttctca
     2101 aagtcttcca gcaacacggt ccctttaccc acaccgatac tcgctgccaa tcccacactg
     2161 gtcggttcat ggcacatgtt ggagcagtcg ggaaagttat tgctcccgta ttcacgggca
     2221 aaaagctgat aaagaaaggc agcttcattg gaagtgcgcc ccgaagtata gaattcaacc
     2281 tgattgggat cactatagct ttgaaggcga gcgccaattt cgtcgaaagc ttgttgccag
     2341 cttaatggct tgtaacagtc gctgacgtca tcatatttca aaggctgagt gagacgtccc
     2401 gcagcctcaa gctcgtgatc tccccaggta agtaatgatt gaaccgtatt ctcagcaaag
     2461 aaagaggcat ttacctgctt atccgtgact tcccaggcga ttgcttttgc gccgttttca
     2521 caaatgtcga atgacgcact gtgcttagga tctggccatg cacatcccgg acagtcaaag
     2581 ccctctggct tattcatgtc aaacatggca ataacatcct ggcgtatatc catctgctta
     2641 cgtactgcat tcgctacgga tttaacagca ccccaaccac cggcagctcc ctggtaggat
     2701 tcaatttttt tcttcatctg cttatcctgt gtggaaatga attattagga tttgtaatag
     2761 cgatgatgca aagtatatac aaagggttta tcgtcaacat gaatatataa gagtagggga
     2821 ctactctctt actattttta ctctattatc gttaaataat agagtagtat aaatacgata
     2881 agttaagcat catctctcat agggtttaag ttaacataat tctcaatata aaaaataaga
     2941 ctctggcttc aattgtgcgc ggattttctt acaagtgtag gataatagcc ttcacaaaat
     3001 aattatccgc gcaattttca tgaatggagc gcatagacta atgtcaaaat cacaattatt
     3061 catagataaa agtgacacca atgactgact ggacggctcc ggctgcaacc ttgtttcctg
     3121 tttgtccata ggttgcgctt aaacccagag gtactttgct tttatttact gtccccaggg
     3181 agacattttc tcctgttgcc agtgtttttc cattacgcat cagagagaca cccacgcccg
     3241 atgcttttgt tgcatctggt gctgtgttag caaacacttg tcgggcactg tcagtggtgg
     3301 cacccgaaag ataaaatgag agcttttgtt cgctagagca atatacacca aggggtattt
     3361 ctgcgcttcc aggaaagtcc gggagattca cggtgacatt acgactatca acggtgcaac
     3421 cgcctgttgg cataacaaca ctgttatttg aaataatatt ccacgtaaaa ttacgcggat
     3481 ttccgcttcc taacgtggca attttataca tatgaatacg ggcaataact tcccctgctt
     3541 ttatgacaac tccaccagcc gctccgactg gggtaatata gagttttaac ggtaatggca
     3601 tcggtgtctt atcaccaata tccaatacgt tggtatttgt cgtcaacggg aaagggtagg
     3661 tcacattatt ccagtacagt gatcctttat atgactgtag ggagcctgcg aaagctgatc
     3721 cttgtaccag gtttatatga tcagtgtcgt accagccgcc gtaatcattc cagcaactta
     3781 tatgctgaga caagtctaca accagattct ggcccggttg tattacagga tcgagattaa
     3841 cataaacaga ggttgtacct gctccaatac ttgacccgcc atcgacatta caactgaagg
     3901 caaaaacttt gcctgcaatt aatagaagat aaataccgaa caggaccttt atactgaatg
     3961 ttttacccat gatatatcct aaggttaaaa aattgattta aaaaggagga aaataaaatc
     4021 aaattgatgt tattgccagg tatagatcac attgattagc gcctcgatcg taccctggct
     4081 tgcgtttcca ttcaccgtga tagctcttgc cttaagtgga aactgtgcat tacgagtgat
     4141 ctcatcaaca ataaccgttt tgctatcgcc attttttaac gtcgcatcct ggtcatccct
     4201 tagctctatc tgaatatttt cggcagtacc ttcatttttg taataacccg tattgtcagt
     4261 tgaacctgtc acgattgccg tcactgtact tgtttcaatc ggacaatcgg ttaatgacaa
     4321 agtaatattg tgccagccag atgcagaacc aggttgttgc agattgcgtg tataaagatc
     4381 cccgagatta acgttagctt ctttggtttg aatagtgcat ggtttagcga ctacccgacc
     4441 attaacagtg atagtgacat cggcggcctg gagagttgct gtgaataatg tgcaaaacgt
     4501 agctaacagg aacctcttgt gaagtctttt catatctgac tctgattatt gaaattcaag
     4561 ggtaaaggtt gccgaagcgc tcaccagtcc aggattgacg gacttctgag ttgacttcaa
     4621 acgagcggag taaggcaaaa tattgttctg atctggtacc aatgggatcc actgcatccc
     4681 ggcatgaagg tcattcaatt tcaccggacg catatttccg tccagtattt ctatacccaa
     4741 accggaggcc gtattgcttc cttcatctag tttcaacaga gtattatttt ctgcatcctc
     4801 aataccgtta aatgcaaccc gaacttccgt tgtccctttg ctgcattcac ttaatgtaat
     4861 ctgaaaaggg acgactggac ttgtgctacc ggtcgttgga aattgtctgg cactgttttt
     4921 ttggagatct acggtaaaat taagcgaatc cgatgagact gtgcagccat aatcgaggac
     4981 gcgcccgcta attttaataa cactatctgc ggataaagca gaataggtgg ttaaccccag
     5041 acataaaccg aggaaaataa tgttattgta tttcataatc tattgttcct tagcgacaga
     5101 ttgctgtctg ctggttcagc aaggttccag gagagactac aggaagcttg taatcgacaa
     5161 tacagtttga gtttttatca ttgccccatg aaacctgtaa tttccctgac tgtggaagtc
     5221 cagtcagata aacctgacca ttttccgcga caatgctgcc atttttattc tctccgtgag
     5281 tgacaattgc accgaatgga acgcttttat taccgtactt caacgtcatt aatactttcc
     5341 cgccgatttg tgcattaaat gttgctctgg caatagcacc gtgagttggg atgacagtga
     5401 ctacggtttc atccagttca acattatctg caagggaatt cgcgttaaga gcgacacgat
     5461 tttctctata ttctgtcgca aatggtaata tggcatagcc acgccagtcg gtatgaattc
     5521 cggtctggtt ctctattttg acattatcag cgccagtagc cttaaccaga accattgtgt
     5581 cgcccagcgg ctgtccaaag gtgatgccat cagcatgagc aataatccca ccactcattc
     5641 cgtaataaat ctggctgctg tcaccactcc gactgtaacc gacattcgta ttagcgtaag
     5701 ctccacgata attaagagaa ctgtaaccac tggtgccaga cgatgtatta cccccttggg
     5761 tgttaccgac ctgaacgcta taattcaggt tattatccgg cagcagagtg ccataaaccc
     5821 ctgtggattt gcccctatat ttccagacac ctgttatcac ttaacccatt actggcttgc
     5881 tgccgtagat attcccgtgg cgagcgataa cccagtgcac tatgcggatg ccattcgtta
     5941 taatgctcga acgcctctgc aaggttcttt gctgccgtta acccgtctgg tttgggcatg
     6001 acactgatgt agtcacgctt tatcgttttc acgaagctct ctgctatgcc gttactctcc
     6061 ggactccgca ccgccgtgct cttcggttca agccccaaca tccgggcaaa ctgccgtgtt
     6121 tcattagccc ggtagcatga accattatcc gtcagccact ctactggaga cgccggaagc
     6181 tcgttgccga agcggcgttc caccgctccc agcatgacgt cctgtactgt ttcactgttg
     6241 aagccgcccg tagtgaccgc ccagtgcagt gcctcacggt cacagcagtc cagcgcgaac
     6301 gtgactcgca gtttttctcc gttatcacag cggaactcga acccgtcaga gcaccatcgc
     6361 tgattacttt ctttcacagc cactctgcct gtatgtgccc gtttcgatgg cggtacagca
     6421 ggttttcgct caagcaacag cgcattctgg cgcatgatcc ggtaaacacg tttggcattg
     6481 atcgcaggca taccatcaag ttctgcctgt ctgcgaagca gcgcccatac ccgacgataa
     6541 ccatacgtgg gcagctctcc gataacatgg tgtatacgga gaagcacatc cgtatcatca
     6601 gtgtgacgac tgcggcggcc atccatccag tcatcggttc gtctgagaat gacgtgcaac
     6661 tgcgcacgcg acacccggag acaacggctg actaagctta ctccccatcc ccgggcaata
     6721 agggcgcgtg cgctatccac ttttttgccc gtccatattc aacggcttct ttgaggagtt
     6781 cattttccat cgttttcttg ccgagcaggc gctggagttc tttaatctgc ttcatggcgg
     6841 cagcaagttc agaggcagga acaacctgtt ctccggcggc gacagcagta agacttcctt
     6901 cctggtattg cttacgccag agaaataact ggctggctgc tacaccatgt tgccgggcaa
     6961 cgagggagac cgtcatccct ggttcaaagc tctgctgaac aattgcgatc ttttcctgtg
     7021 tggtacgccg tctgcgtttc tccggtccta agacatcaat catctgctct ccaatgacta
     7081 gtctaaaaac tagtattaag actatcactt aaataagtga tattggttgt ctggagattc
     7141 agggggccag tctaccctga taggttggtc atgccgcctt tcaaatcgtt tgacatactg
     7201 taactggcgt ttgaattacg aaatgccgac tgactgtctg tacgcatcca atgactaaag
     7261 ggaacattaa gcgtaaaagc gagtaaatga tcccgatcgt tttgccatat attattggaa
     7321 tagctgtaat tcagtgaagt cgtaatatca ccaaacggca catttaatcc aaatgatatt
     7381 tgctggtcgc tgcgtgacgt gttccagtaa ctttggcgac tggcactgaa aaatgtcgta
     7441 ccgtaatttc caagctgctg agagatgctt atttgttcct gaccacgctt actgtagaac
     7501 agattaaaat aatcaataaa tagtgtctgc tcactggtgt ctccggtagg aggcttgacg
     7561 gtgtaaccac tcattcgact gtatgcactg tcggataagt tataaaaacc ttgcgtagaa
     7621 tagcgatatc ctgcgacctg gatattggtt cccgtctggt agaaggattt gctgtagacg
     7681 gatttaaccg attgtccgct gtgacgggtg tcatctgcaa gtgtcgtatt ggcctgcgta
     7741 atatcgaaag aaaaggcacc aaaaagtcct aaatctttac caataccaag gttgaaggcc
     7801 tgataatctt ctgcaatttg cattccgcca taaggtgtcc agtttccttt cagaccatgc
     7861 atcaagcttg cttgtacgaa cttgggggtg ctttgcaggt tatttccact acgatattcc
     7921 cccatggcaa gtgcataacg cgtatatcca gcacgttgga gaaccggaac agacgaataa
     7981 ggaacatata atgtctgaat actgccgtct gcctctttta tggttacttg cagatcacca
     8041 ccattggccg cagagttgat atcatcaata gtaaaagggc cgggtgggac agtagtctga
     8101 taaacatcgt atccattctg ttttacactc acttgtgcgg tgccacgagc aataccatga
     8161 atcaccggag cgaaaccatg ttggctatcg ggcaacatcg cttctgttga atttatttta
     8221 aggccacgaa agttcacact gtcaaaaata tcgccatcgg tataactatc acccactgtt
     8281 aaccgtgagc gtaacggaat aatgtcacgt tcagccgacg tattgatatg ctgccattta
     8341 ttgctgtcag atgaattgct actcccactg ttataactcc acgttgagtt atcgcgcagt
     8401 cgccaactac caatgttgat gccactctgt aaattcaaat atgcatagtt ggattttcct
     8461 gcattatggt tactacggtt attaatagaa ttaccattaa aactatagtt tagtagcccg
     8521 gcatttattc cttcttccca cagatcagga gaaacatagc cgcgagccat tctgccgaca
     8581 tatatttgcg gaacggagag tgacagatgt tgctgaccag catcaaactc agatgatgcg
     8641 tcagccaaac gatcctgaag tggtgtgcat gcgctgttgt ccggcacatg tttttctgcc
     8701 gaatgttcct tgctgtctaa taatgctgat ttttttatgc caagactgac aagaaggtct
     8761 gtactcaggc atgggatgag atctgcgtta ttatcatctg caataaaagt aatatcccga
     8821 ctagccataa attcgtcgtt caaataaatg tcgacccgat atgtaccagg tggggcctcg
     8881 cgtcccttag taaaggcaga taaatcgaca gattcagcaa ggtcattgct taaaaaccgt
     8941 ggattaaaat aatactctgc gtcaacggga aaaaccgcag ttgctaagcc aagggcaaag
     9001 gtaagccgtg caaagcgagg cagtattaac acctgatgca tacgatatgc cttaaatgcg
     9061 gcacgaaagg ctgtcatttt taatcccgat tgagtttatt attaattagg tcactggctt
     9121 actgcatggt tgccttaata cgcggagtta atgcaccgta atcattgatt gtctgaaaac
     9181 tgatatcccc ggttacagca tgcgggatat ccaccgatac ttcacctttt ggcggcacca
     9241 tggtgtttgg gagattgcta tttcctgctt tcatatttgt tacggtaatg aagtagggcg
     9301 ttggattaat aagagtgaat ttgctaccgc tgcgacgaaa ccgaagcatt gcaggcgctt
     9361 cttcaggtgc cattgccaga tgcgtggggc gataaaacat ttttatgcgg ctaataattg
     9421 ccagctgtaa tgtattctca ttcttctggt ctttttccat agcagggata gctttaacat
     9481 taacccagaa caggctttca cggtcgcctg gtaactgatg attagtggcg ttgattatcc
     9541 gaagcgtatt ctctttttta ccttgcatgg aaaaaagcgg tggggtaatg acaaactggg
     9601 tatctttctg gtcacccgca ttttctatcc atgactgaat gagatagaca ctggcaggat
     9661 cattattcgt gacaggcaat aaaacctgtt tctggtttgc cggataaatt acccgcgtcg
     9721 caccaagtgc gacagctgcg ttggcaatgg tgctatggca agcaaagagc aataaaagta
     9781 acactgtcgc cataaaggaa acagagtagg gggtacgcgt agtttgcatc tgatgcattc
     9841 tccatgtgtt aagaggataa gcggggaaac cgttacatca gacaatcggt aaatattgct
     9901 aatacattgg cgagtcgccg ggtttccaga cttaatgcgt ttgtaataaa tgttggttct
     9961 cttttgatag gaaaaccttg ctaccaagta aatattttaa ataacagcaa gtttaactac
    10021 tgttgtttat tgatcgttta gatattatgt gatttttcat accgctccaa aatacaaacc
    10081 atttttcatt aataatgaga tgaacattgg tgaatttctc gggtgtgcgt agccatgcca
    10141 cacacaccct gtttcaattt cagatgacgc atagtcacct taagagtgaa ttatagatat
    10201 tgaactttga aggtcgcatc agcgtttgca gtaccagcta ctgactgccc gagagcaata
    10261 taacgagcct ggaacggcag tatattcgtg ccatcaatca agtcagtttt agcgctaaaa
    10321 gtagcaccat caagtactaa tacctcccct gtacggtcca aaatttggat accgacattt
    10381 tgggcagagc ctgccgctga actctgcagc gctaatgtat cgtcattact ggtgacagta
    10441 gtacccagga atgcaacagc tgcattactg gaaacattgg tatcacaatc attcagcttg
    10501 atattgaagc caacggcgga gctgaggtca cccgctttag ccaggcgaga agaacggacc
    10561 tgacccagct caaccgtttg gttcattgat tctgaatcaa tcgcacaagc ggcatttacc
    10621 acttcacctt taaaatgaat aacaccgcca ttaacagtgg tcgttactga ttcatcaccc
    10681 tcggctgcgc ttaccgcagc agacgacatc gccaacacag aaacgacaat aataccgaca
    10741 tgtttgagtt tcataattct ccaaaattta tatttaagta gctgaataaa gagagttaac
    10801 aattgagtta ttctgttatt gcttataaaa caagaatcat tcttgattgg atattgccct
    10861 aagaatatag tttccatggt tattacattt cacgaagaac gataatatct ttaaattaca
    10921 aggacaccta ttcacaacag gtttattgct gcacttgttg tagagagtat ttgtaaaaat
    10981 attttatgtc gctaaataac atgacgattt cattatgcat gttatttata aacagcgact
    11041 aaacagaata ttattgcgaa caagggaagt gtcaggaaag gtcaagaggt actaatagtt
    11101 tatatggcta taaacattag ctatggttaa tatttgcctg gtgaaactaa cacagaattg
    11161 ctctaaacag ataagtgcag ggtttattaa agctgaccga cagacttttt taacctggta
    11221 atatctctct taggtgactc tccaaacatc cgcgaatatt cccgactgaa atgggataag
    11281 ctttcgtaac cgacaacata ggctgcagtg gtgacatcgt aatgttcatt cagcatcagt
    11341 cgcctggctt cattgaggcg tagccatttc tgataccgga gtggactcat tcccgcaagc
    11401 tgtctgaagt gttgatgaaa agagggggtg ttcatttgca cgcggtttgc cagcctgact
    11461 cttcaacgca gggaagatgc ggcaaacggc aaacctgcat accagccagc ctatatccgg
    11521 tatgcacagg ccaatcatga aagaggagag gctttatgtt ctggaaccct gaagtaataa
    11581 cgacgaccgg aaaatcgctt cgcatcactc aaattaaatt taacataata tacattatgc
    11641 gcaccaacat aaaccaaggg agaatccagt cgttggcggt tcatgattgt tctgctcatt
    11701 aacaacgacc aaaaccccat atcttactta ataccgtatt ctcggcttaa ccgaccgtgc
    11761 aacctcaaca cattggtttc aactgccgtc accacgttct ccggaaaatc tgtcggtaat
    11821 gaagtcttca cgttatccag tgctgccgga atcattctgg caaagtcact caggatttca
    11881 tgcatctgca cttccgggaa tttcagcacc tttgctgtcg ccagaaaatg tcgcggataa
    11941 attttatcga ttgccgtttt tttgcctttg gatgcgttaa gccccattgc cagtttgaga
    12001 tcgctgatgt gtattcccgt accgccaagg accggaaatg ccgaaatgat gtcataaaat
    12061 ggtgtgagcc gataactgcc gccagcctga ataaatacgg agaagttttt tgcatggcca
    12121 tctgttaacg gacagatatt gctggcattt ccgcgtctgt ttattgttgc ccgccgtacg
    12181 gagtaattat cacgtcagct ggtaatggcg atcgggaaga attttcgctg gaatttctgc
    12241 ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat ccagcggtaa
    12301 atcattgttt tcagtaccga agggatcttc cagttcttcc gccagacaat ccagcgaaat
    12361 aaaagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcagat ccacgaccag
    12421 cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca gggtgtaggc
    12481 aaagggaatt ggcgtgtagg caatgcgctc gcagcctgcc agaactgctg aaatatcatt
    12541 aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgcccattgc ggcgctgaac
    12601 cgccaaccac tcccccatta ttaacaagat acggttagct ggagagttcg aagccagtac
    12661 acgctgaaga tcttccgtct tgagatattg agccagcact tccgcctgtg gttgtttgcg
    12721 taatgtcatg cgtaaacagt gagcgaaggc gatttgcagc cgggcaaact cccttacact
    12781 tgccgaatct ggcaatgtcg tttttacctc gcgcagtaac gaccgtgagg caatcatcaa
    12841 ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat tacgaaaacc
    12901 aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga atttaatgcc
    12961 caaatgcgtg taccagggca gcatgaaaat aacagcgatc gaaaagagaa aattgagtag
    13021 taagcgcgag gatatcttgg ataatactga accgtgccag acaaaaatac ggcgcagcca
    13081 gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg ccctattaaa
    13141 cgtgattacg atcacattct caagacacta cttacaaatt acctacaagc ttacaacatt
    13201 tacaggtgtg gcatatggca cctgttaatg ataaaggcac ataggatgtt gtaactaata
    13261 tagtgaaata agaaacccgg tcgaaaccgg gttcagagta ttagcacaac ggacgtacag
    13321 cttcgcgcat tcctttttcg agaatcgcat ccaggtcatt agcaacctgc tcgaccagac
    13381 caggcacttg cgtcaggtcc tgctcccagt ggtctttttc agccaacaca atagcaacca
    13441 gttcctgggt gccgataaca cggtcacgat gctggctcca gagttgctga taacgttcca
    13501 gccagtgggc atcgtcctgt accggatatg tttctccatt acgctcaccg cgataaaacg
    13561 caatcaatgc tgctaatgcg aaagtaaggc gcgccggaag tgtgccgttt gccttctgcc
    13621 ctgccagcag ctgcggcagg atacgggtgc ggaacttggt catgccgtta agcgcgatag
    13681 acagcagctg atgcttaatg tacggattac ggaaacgacc ggtgactgca ctggcgaaag
    13741 attccagttc atcacgaggc aaatccagta ccggaataat ctcttcgtag atggcttttt
    13801 caacgaatgc acaaatttca gcatcgttca tcgcttcacc tacggtatcc agccctgcct
    13861 ggaacgccac cggcaccagc gcggtgtgcg caccgttgag tattgccact ttgcgttctt
    13921 tatacggttt aatatcgtca acaatcagca cgttgagcgg atatttgtcc agacgcagtt
    13981 cagtcgctaa ggatttcggc ccctgaatca caaacaggta aaagtgttca gcggtgtcga
    14041 ggaaaccatc gtgataaccc agttcttctt ccagtttagc cacttcatca cgcggatagc
    14101 cggtaacgat acggtccacc agcgtagaac agaagctgtt agcctgatcc agccattgaa
    14161 taaatgcttc tggcagtgcc cactcttgcg catagcgcag caccagttca cgcaacgcgt
    14221 cgccattgta gtcgatcaac tcacacggga tgatgatcca acctttatcc agcgcaccgt
    14281 tgaaatggct aaaacgttcg aacagcagac gggtcagttt tgccggataa cttaccgctg
    14341 gcgcgtcatc gaatttatcg cccgcatggt agctgatacc cgcttcggtg gtgttagaga
    14401 aaacaaagcg catttccggg ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga
    14461 cgctgatttc acgattaaca gagcgaatca ggcgcgcgtc gctgaccgct tcacccttct
    14521 cattcaggcc acggataatg gtggtataaa gaccatcctg cgtgctcagt gacggcggga
    14581 atgaagtttc aatcggacga acaacgacca cgccagaatt cagatcggtg tgctcattca
    14641 ggagatcgat ttgccagtca acaaaggcgc gcaggaagtt accttcacca aactgaatga
    14701 tacgttctgg atactgtgca ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt
    14761 cccttctgat tagtcataca acctgtttga attggtacga caggttagca aactttaata
    14821 cgccgaaccc ctgttttgat caactcctga tgattaatga gcagttttat gagaaaagtg
    14881 tggcgcggat catggtttga tcgaggaaac aggccttttc ttggatcata aagtggtaga
    14941 acatattgca tttaattcgc tctgagtaaa taaagatgtc agacaacttc ctcaccgtaa
    15001 cgcatagtgc tggtacggtt gcgcccatct ttcttcgagc gatacagaca cgtatcggct
    15061 tcgaccatca atttattaaa gtcatcagtc agcgttcggt acgatgcgca accactaccg
    15121 acgccaatac ttaccgtgag ataaagcgtt ttttgttgcc aggtgaacgg ttgaagttca
    15181 acgcctttac ggattttttc cgccattagc aggccatcga caggattcac cgacggcact
    15241 gcaacagcaa attcttcgcc gcccattcgc gccaccagcc ccttatcacc gacaatcttc
    15301 tgaatatgct gggcaaacac gcttaacact ttatcgccac attcatgtcc atagttatcg
    15361 ttaatgcttt tgaaatagtc gatatcaagc agcatgaccg tcagatgttg tgtctgtttc
    15421 agcgatggac ttttcagcgc ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa
    15481 tcaaagtcgg ctcgcagcgc aacttgcttc attagcgaat tgatcgctgc cacgctaaag
    15541 gaaaccataa ttgggcatat cgccatcgtg gcaataccga gacgtgcgga gaacatttgc
    15601 ggaatggaga acggcgaacc gaccgagata tcaatcaccg aatttgccac cagtacgact
    15661 tccaccgcac cggtgacaaa ggtcaacaga catgttacct gcggcgtata gcgcactgca
    15721 caccagatta atgcagggag cggaaacgcc agactccccg ctccgccaat gactaccgag
    15781 gcaataaccg acacaataag cgcgatagca ggcatcatct gctctgcttt aaagcgcggc
    15841 agtactccag gaattgccaa cgtcagcata caaggcacga tcaacacgcc cgttgagaat
    15901 tgctcactaa accagtcggc aagcaaaggc cagaaatcca gactatcaat actgaccgaa
    15961 ccaatcgccc cgacaatagc gcataataat gcggcaagca gacagtaatt aaatagccgt
    16021 aaggcactta ctggctcata cttatttttt cccagacgct tatcccgcgc gaccagtaag
    16081 gcgacggtaa caataaacat catattggag aaattgatag ccagtgaaac cagcccccat
    16141 tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata gtaatgcagt
    16201 cgattaagcc agacatagcg ggcaaacacc cccgccatta cgccattcag cggccagaaa
    16261 agagaaagtt cgtgaaccag tcgtaactct gcaccaataa aatagaacag cgtggttagc
    16321 acaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa
    16381 atgggctgta catgcattaa ttatctgtcc atacaattgc gggtggcaat tatctcaacg
    16441 caataagtaa attcttagaa aggatagcat gttcgctgac aacggcggca actaaagata
    16501 cctaatgaat gccattttat cgatttccgt aattatcatg gctgattcat ctgtataacc
    16561 agccagtggt agattaccgt cacaatataa cgttgttgct cctcacttaa cgcttccccc
    16621 tgaggaatgt tatgccactt agccagacaa ccgcgacaac aggtcgcggt ggcgtgttga
    16681 gcgataaaaa ccggatgccc gcgcatgggg gtttgcttac cgtcgttagc cggtaatgcc
    16741 ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt caatgactgg cgcgcctttc
    16801 tccagacaat actgacgctc tttcacaccc aaacgaaagc gagagcgaaa ttttgaacgg
    16861 gataaacgcg cgaagagtgg atcgagagac tgcattaata aactgaacgc cctaattgtt
    16921 tcgtcaattg ttcaaggact gcaatgcccg caagcgagtt accggcatca tccagttccg
    16981 gactccagac tgcgatggcc atttcatgcg gaacaatcgc cacaataccg ccaccaacgc
    17041 cagattttgc cggtagtccc acccgccagg caaactcccc cgcgttctgg tacataccgc
    17101 tggtcgccat cagcgcgtta atttgccgcg cctgcattgg cgtcaccaca ggttcatcaa
    17161 tatgaatagc tttcccctga ttcgccagaa agacaaacgt ccgggccagc tctacacagc
    17221 tcattttcag agcgcagtaa tgaaagtagt tttgcagaac ggttgtcacg tcatgatgga
    17281 aattgccaaa cgacttcatc agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt
    17341 cggaacgcgc taccaccgta tcgtaggaaa tatcagacac accacttaag ccgcgcacga
    17401 cttccagcat acgttgccga ggtgcgctta atcgcccttg caacatatcg cagaccacca
    17461 gcgcaccggc attaataaac ggattacgcg gaataccctg ctccatttcc agttgcacta
    17521 aagaattgaa tggtgatcca gacggatctt tgccgacgcg ttgccagatt tcctcttcgg
    17581 agtaatgacg catggcgaca acgagactca gcactttgga aatagactga atggaaaaac
    17641 gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac ggtacagata gcaatcccca
    17701 atcgggaacc gtctaccgta gccagcgccg gaatataatc cgcgacttta ccctgaccaa
    17761 tgagcggccg cacttgccgc aagatgtttt ctaaaattgc attatccatg gcgactgcca
    17821 ctttctactc ctggaccgca ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt
    17881 agcgtcagat ccggtctttc cacaccgtct ggatattaca gaattcgtgt aagccgaaat
    17941 gggaaagctc acgcccaaag ccactcttct tcacgccacc aaaggcgact cgcgcatcgc
    18001 tggcacaata accattgata aacaccccac cgcattccag acgtgccgcc atctgtctgg
    18061 cctgtgtttc gtcggtggtg aaaatggtcg ctgaaaggcc gaactcacta tcattagcca
    18121 gttccagcgc atgttctgca tctttcgcag tggtgattgc cgcaacgggg ccaaacattt
    18181 cttcccgaaa cgcggtcatt tctggggtaa cattcgccag aaccgttggc ggatagtagt
    18241 tacctgcccc agccatcttt tccccgccca gtaacaaacg cgcaccctgc gccagtgttt
    18301 tttccacctg atgatgcagc tcatcacgta aatcaaaacg agccattggt ccgagagcgt
    18361 tctcttcatc acggggatcg cccattttca aggctgccgc agctgccaca aaacgttcgg
    18421 taaatgccga agcaattccc tcttcgatga taaagcgttt tgccgctgca caaacctgtc
    18481 cggtattctg ataacgtccg gctaccgccg ttttcaccgc cagttccaga tcggcatcgt
    18541 taagcacaat aaacggatcc gaaccaccca gttccagtac gcattttttc aatgccgctc
    18601 cagcctgtgc gccaatagcc gctccagcac gaacacttcc ggtcactgtg acagcagcaa
    18661 tgcgcgagtc tttaatcatc agactgacac cgtcgttgtc ggcattcagc cagccatata
    18721 cgccttgtgg gatacccgca tctttaaaca cctgggcaat gagctgtgca cagcccatca
    18781 cattcggcgc atgtttaagt aagtagccgt taccagcaag aatgatggga acagcgccac
    18841 gcatcacctg ccataacgga aaattccacg gcataatcgc cagaatcgtc cccaacggtc
    18901 gatactcaat aaccgcctgc tgattttcca ccagcgtagg ttctgccttc agcattgccg
    18961 gaccatgttc tgcataccag tcacacaaat tcgccgattt cgccacttca gcgcgcgcct
    19021 ggttgattgg tttgcccatt tcacgggtga tcatttgcgc catttcttcg ctacgggcgc
    19081 gcagagcctt accgatatca cgcagttttt cagcacgata atctatattt gtctcgcgcc
    19141 agtcgcgaaa gcctgctgct gccagctgaa gcgcgttttc gatatcgtca acaccagccc
    19201 acggcagcac agaaagttgt tcaccggtgg caggatttat cgaaattgca tgagttgccg
    19261 gagtaatggt catcggggta tctcctttat gagtcatggt atgaagatac gcagatttac
    19321 tcttgcttta aaatgaataa tattaagcca cttattcacg aatcgagaat gctatggatc
    19381 tgacccaact ggagatgttc aacgcggttg ccgaagctgg cagcattacc caggctgcag
    19441 caaaagtgca tcgcgtgccg tcgaatctca ctacccgtct acgccagctg gaaacagaac
    19501 tgggggttga tctgtttatt cgcgagaatc agcgtttacg tctctcgcct gccgggcata
    19561 actttttacg ctatagccaa caaattctca cgttagtgga tgaagcgcgg agcgttgtcg
    19621 ctggcgatga gccgcaaggt ttattttctc tgggatcgct ggaaagcacc gctgcagtgc
    19681 gcattccggc cacgctggcg gaatttaacc gtcgttatcc caaaattcag ttttcacttt
    19741 ccaccggccc ttccggcact atgctggaag gtgtgctgga gggaaaactg aatgcggcgt
    19801 ttattgatgg acccattaac catactgcca tcgacgggat accggtatac cgcgaggaac
    19861 taatgatcgt cacgccacaa ggacatgcgc cagtaacccg tgccagtcag gtgaatggca
    19921 gtaacattta tgccttccgc gccaattgtt cgtatcgtcg ccacttcgaa agctggtttc
    19981 atgctgacgg tgccgctccg ggaactattc acgagatgga gtcttatcac gggatgttgg
    20041 cctgtgtggt cgcaggagca ggcattgcgc ttattccacg ctcaatgctg gaaagtatgc
    20101 cggggcatca ccaggttgaa gcgtggccgt tagctgagca atggcgttgg ttaacaacct
    20161 ggctggtctg gcgtcgtggt gcgaaaacgc gtccgcttga ggcatttatt caactgctgg
    20221 atgcgcctga ctcggcaaaa cagggttacc aatgagctat ttttgatagt tcatacggct
    20281 aatatgctct atatagtgat gttccgatga cttatgacta tatggggcaa atatggttac
    20341 gccagtaagc atcagcaatt ccatatctct tcctgatgat tttcccgttc gtaatattgc
    20401 acctcaagta aaagaagaac aggccccctt cctgatcgtc attaaaatgt gatgttaaca
    20461 actctctgat gcgtgacaga acaaccgtca gagagatgga aatgcgatcc agatcacaaa
    20521 tgcattgtat tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac
    20581 ccgataatct gttatgacaa caaacactgt ttcccgcaaa gtagcgtggc tacgggtcgt
    20641 tacgctggca gtcgccgcct ttatcttcaa taccaccgaa tttgtccctg ttggcctgct
    20701 ctctgacatt gcgcatagtt ttcacatgca aaccgctcag gtcggcatca tgttgacaat
    20761 ttatgcatgg gtagtggcgc taatgtcatt gccttttatg ttaatgacca gccaggttga
    20821 acgccgcaaa ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt
    20881 tttatcgtgg agttttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc
    20941 gattttctgg tcgattacgg cgtctctggc gatccgtatg gctccggccg ggaagcgagc
    21001 acaggcattg agtttaattg ccaccggtac agcactggca atggtattag gtttacctct
    21061 cgggcgcatt gtggggcagt attttggctg gcgaatgacc ttcttcgcga ttggtatcgg
    21121 ggcgcttatt acccttttgt gcctgattaa gttacttccc ttgctgccca gtgagcattc
    21181 cggttcattg aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt
    21241 gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt
    21301 acaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg
    21361 tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt
    21421 ggtgagtaca gcgattgcgc tattgctggt gtgcctggcg ctgctgctac ctgcggcgaa
    21481 cagtgaaata cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg
    21541 gcttggtatg caggttaaag tgctggcgct ggcaccagat gccaccgacg tcgcgatggc
    21601 gctattctcc gggatattta atattggaat cggcgcgggt gcgttggtag gtaatcaggt
    21661 gagtctgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgctgc
    21721 gttaatatgg tcaatcatca tttttcgccg ctggccagtg acactcgaag aacagacgca
    21781 atagttgaaa ggcccattcg ggcctttttt aatgatacgt tttaatgatt tccaggatgc
    21841 cgttaataat aaactgcacg cccatacata ccagcaggaa tcccatcaga cgggagatcg
    21901 cttcaatacc ccccttgccc accagccgca taattgcgcc agagctgcgt agacttcccc
    21961 acaaaataac cgccaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag
    22021 cgaaggttga actctgacgc actgtggagg ccgagctaat aatcatcgca atggttcccg
    22081 gaccggcggt acttggcatt gccagtggta caaaagcaat attggcactg ggttcatctt
    22141 ccagctcttc cgacttgctt ttcgcttccg gtgaatcaat cgctttctgt tgcggaaaga
    22201 gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg
    22261 aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa
    22321 atacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg
    22381 ccaggccaag aaataacgcg acagttgtta atgggttagc taacggcagt aacaccacca
    22441 gccccaggcc tattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg
    22501 tttatcagcg aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt
    22561 gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattc
    22621 atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa
    22681 ctaatgtgaa aaataccagc gatctgttca atgaaattat tccattgggt cgcttaatcc
    22741 atatggttaa tcagaagaaa gatcgcctgc ttaacgagta tctgtctccg ctggatatta
    22801 ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg
    22861 aactgaaaaa agtgttgtcg gtcgacctgg gggcactgac ccgtatgctg gatcgcctgg
    22921 tctgtaaagg ctgggtggaa aggttgccga acccgaatga taagcgcggc gtactggtaa
    22981 aacttaccac cagcggcgcg gcaatatgtg aacaatgcca tcaattagtt ggccaggacc
    23041 tgcatcaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc
    23101 ttaagaaagt cctgccgtaa acgaaaaaag aggtatgacg atgtccagac gcaatactga
    23161 cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc
    23221 actggagaaa gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa
    23281 aaaagaaacc ggtcattcat taggccaata catccgtagc cgtaagatga cggaaatcgc
    23341 gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga
    23401 gtcccaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata
    23461 ccggatgacc aatatgcaag gtgaatcgcg ttttttacat ccattaaatc attacaacaa
    23521 ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttta tccgcaatag
    23581 caactgcgct tattctcttt tctgcgcagg gcgttgcgga acaaaccacg cagccggttg
    23641 ttacttcctg tggcaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt
    23701 taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc tattacaatc
    23761 aacacgctat gtagttattg tcaggctctg atcacagagc ctgacatttc cgtaatatct
    23821 tcctgaagtc tcatatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg
    23881 atgctttacg tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca
    23941 ttccgtagcg tttttttatt tctcctgttc cggcagaaac cagatcaatg caaccccacg
    24001 gggtgtagcc aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat
    24061 gggaggaaaa gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt
    24121 caaccgcacc aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc
    24181 agttgagtga ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga
    24241 tataagggtt gctgaccaaa tctcgtggtt caacataatc cagttgcgga ttatctggcg
    24301 aaaattgcgt cgtaaaagac atgtagtagc taaagccgat aaagtcgaca caacctctgg
    24361 caagaatcgc gttatcatct ggtgtgatat cgaggttgaa tcctttcctt gcaaagtaat
    24421 tcagcatatg ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat
    24481 gcatcgcttt cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatggggc
    24541 acatagcaat catacagccg atattaaatt caggattgat cagttttcca gtctgtaccg
    24601 ccagggcact ggcaactaac tcataatgta ccgcctggta cattatttgt tcgcgctcat
    24661 tgagatcttc ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc
    24721 tgaaattcac ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat
    24781 agcgcgtgaa gaccgttgat gcgaagtgga taaaaaagtc gatcagttta cggtttcgcc
    24841 agccaccata ttttgtcacc agatgataag gcatctcaaa atgcgaaagc gtcaccacag
    24901 gttccattcc ctgcttcagg cattcatcga agagatcatc ataaaattgt aaaccctctt
    24961 cattcggctc ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa
    25021 agcatttgaa tcccatcccg gcaaataact gaatatctgt tttatagcga tgataaaaat
    25081 caattgcttc atgattggga taattaagtc cgtcgataac gccttctgtc acttcacgcg
    25141 gcaccccgtg agcgccagca gtcattacat cagcgatact gatgcctttt cctccttcat
    25201 tccagccacc ttccaattga tgtgcggcta ccgcaccgcc ccataaaaaa tcttctttaa
    25261 atcctgacat acacaactcc ttaactaaat agattaccac cagacttccg cctggatgcc
    25321 ggccataaac tcgtctttgc tgttctcatt aaacttaaaa ttcgataatt cgttatccag
    25381 aatattgata tacgtcccgt agaagcgaat ttctggtcgt gaacctaaaa tactttcacc
    25441 cactttcaat gcatgccaga gtgtcgtttt ataagccgat tcatttagcg ttaccccctg
    25501 ctgagttttg ttctgttgct taaaccagcc taattcaagc cccgtctgat tccatgtatt
    25561 ccagatccag gccggtctta ttacggtgcg aatactgtca aaatcactat gagcgccact
    25621 ttcataacta taaacatctt cgccatgaga atagacaagc gcgttagcca taataatatt
    25681 gtcagtcaga tacatctcgc cctgagagat taaacgccag gcgatcccat tggtatggtc
    25741 accatagtaa tagcgaccat gcgccatcgt attactggca tctgagaaac tggcaaaact
    25801 gctggcatag gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcaa
    25861 gttttgccgt aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgtcgtc
    25921 attttcatta tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga
    25981 caacgtcgca tcatcccata acgggatatt gcgataacga acatccacag aattggtatt
    26041 catctgactg gtacgcgtaa aatcacgaga gtaaacatcg acatcatctc ggcttaagga
    26101 catatcaaac agccctacac caagcgccca gttttcaatc cccacacccg cggcaacatc
    26161 cgtggttaag gttttccagt ccagcatttg gatctcatat tgcgggagtt tatgtttgcc
    26221 gacccagaag tctgcctctc gtgcgaaggg taaaaaacct cgcgttgtca gataaatatc
    26281 actgaactgc atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctg
    26341 acctacgttt ccatcatagg taacgaccgc atttgccgtt ctaccgtcct gattataaac
    26401 acgctgattt aaggtcaggt caaaccaacc actcatctcg ttaccaaaac gtcccagaga
    26461 acccgctgca taagtttgtg gtgagccgtg attaccggtg ccccagcctg agcggaagta
    26521 cccctgatag ctgaatccaa tatcatcctt tatatattta ctgatatctt tcagggtcac
    26581 gctttcgata gtggttttat cgcctttact ttcaataacg gcaatttgct gcgatccatt
    26641 tatagtatgt gctgcggcag acgagttctg ttcaccgttt tcatttttga tgttatcagc
    26701 aacgggagat gtcgctgata tttcggcaag cgaggccgat tgatatttat tttctgtgat
    26761 gcttttttgt aatgtcgaaa gtcggaattt atatactccc agttcattct gtgttgcttt
    26821 cagctcttgt ttattttgcg acaattcatt ttcaagcaat tcaaggcgct gctctatggt
    26881 taattttgtt gccatcgcgt tcaacggaaa caatgctgta ataatagaaa tagacaaaag
    26941 gctgacgtta agcgtcttta tattcataac ttatccttaa gagtgtagta atttcctcca
    27001 gctatcgcac tggagaaaaa atcaggcatg gagctctttt cttaatgaaa taatttccct
    27061 ggctaaatcc tggcataata ttgcattcat caaatgatcc tgtgcatgta tcagaattaa
    27121 attaacttct attttgccac agccttcatc ctgactaatt aacgcggtct gaatcttatg
    27181 cgcctgaaga caggcagatt cagagctggc cattagttta gttgcgccct gccagtcttt
    27241 ttgcccggca caacgtatgg cttccatcgc atcagaacga gcctgtcccg cgttgatcag
    27301 taactccata acaagttctt catctgcaaa catcacaatc tccttcctgg gtaaatggaa
    27361 ttatccgtta gttgtcatgt tattgacttt gttggcggcg ataacaaaag gtaaatagat
    27421 cattgtcgcc acacctaaac ataaaaaacc aaccagtaat gccagccagt ttcccccggt
    27481 tccgaggaaa gcaattaaag gtccgggcgt agtccagggc acggcataag caaccggtgg
    27541 aataatttcc atcgaaacaa agaagtaacc aatggcacag ttaacaatgg gaaccataat
    27601 aaacggcacc atcatgatgg ggttaagtac aataggaagg ccgaacatta tcggttcatt
    27661 gatattgaaa ataccgggga taaatgacat ttttgccaga tcacggtatt ccgcacgctt
    27721 agaagcgata aaaatagcca acaataaccc caacgtcata cctgaaccgc cgcagttggc
    27781 gaatgcatca ttaatagaag tccaggtaat cggatatggc gcgccccagg tagtgccgtg
    27841 ttgagcggcc caggagagat tctccagatt agcctcagaa aaaacggttt ctcgaattgc
    27901 cgacgtggta ttaggtccgt ggatccccag cacccacagg aaattaccca cagcccccag
    27961 gataattacc gcaaagatat tcgttcccat atgtttgagc ggcgtctgga ttaatgtgta
    28021 aatgagatca tttaatcctg ccggggatat caatgtcagg caataattaa gtgcggaaaa
    28081 gaacaccatg acaaaaaata ttggcaataa aactttaaat gaacgcgcta ccgcaggagg
    28141 tacagctgcc ggcatcgtta tggtgatttt ggggtttcga gctaagcgac aaaatatttc
    28201 actggtgatc aatgcaacga taacagcaac aaacaacccc tgcgcgccga gatatttggt
    28261 cgtcaggaat gcctgcccat ctatcatctg gtatggggta taaacaataa aaaatgctcc
    28321 tattgccgtt aatccacata aaagatcatc ttgctcatag ctaatcgcca cattcctggc
    28381 gaccaggaaa gcaatcataa ttgacatgat atttacagaa ccattcatta ccggagtaaa
    28441 aatagcttgt gctttttcaa ggttggggaa aatgctgttc agatgcagta atccggcaat
    28501 aaaaccgtcg ggcgataata tggcaaagtt aattaatata attaatgagc ttgccatgat
    28561 aattggaaac gtaaaaacga atccatctct caccgctgca acatgttttt gtgagtttaa
    28621 cttgatagca actggaacaa gaaaacgttc cattccacgt tcgaatgatg ccattaatcc
    28681 cattttttta tacctgttgt cctggatatt tatacatccc aataaagcat gtatgccata
    28741 tccattttat gatgtgtgaa aagttagttc aggtaatagt ttaataatat tagattctta
    28801 ctcttgcagt agtttcatcg caaattcaag aactgcctgt ccgttcattg tgccatagtc
    28861 tttcatctcg ataacggcta caggcatttt cccctgtgca acggcttcaa tctctggttt
    28921 ctgaaagcga acttgcggac caagtaaaac aacgtcaact tcgttatttt taattttccc
    28981 tttagcctct gtaatcgcca atgcggaaat attaacttca agtgaaatag cggtagcatg
    29041 atcaatcata cgtttaacca gcatactggt tgacatgccc gcagcgcaaa ctaacaatat
    29101 ctttttcata tttttcctta ctggtatata acagactaca ttgtgtaaat atcatatccc
    29161 acagtagcta cacgtcctgg tacattgatc acagtaatac agcaataaag ttgttcaaca
    29221 acagggtgat aattaaacga ctcggccata cgataaacta atcaggtctc aactccttgt
    29281 tttcaatggc tcttaaaagg tgcaaaagtg atttttcaaa agattgcccg cttacttaaa
    29341 tccgagatca atggcaattc atggcatgtt ggtgatttgt tgccgtcaga agcggaactc
    29401 gctgttcgtt ataatgtttc gcgtaatacc ctgcgtaagg cattatccct gctggaaggc
    29461 gaaggaatta ttcacagaaa gcatggttca ggaacataca ttcaaaaaaa gaattttgtt
    29521 gcacacattg atcacatgaa cagtttcagt gaaattgcac ataaaagcgg caaagaggca
    29581 ggaagtcaga ttatgaaatt tgaagtgcag gatgcttctc ctactattgc gactgagctg
    29641 aatttagtga ctggtgagca ggtttattac ataaagagac tgcgattcat tgaggataat
    29701 gcagcacaat tagaagaaac gtggatgtca gtggcacgtt ttcctgattt aaccgtatcg
    29761 catatgcaaa aatccaaatt ttcgtatatt gagaacgaat gcgggatcaa aatcattggc
    29821 acctttgaaa ctttctcccc gacttttcct accccagaaa tcgccagtat tttacggatc
    29881 agcccacggg atcccatact taaaattcag acccaggctg tggatagtaa ctctattccg
    29941 ctggattatt cgttacttta cagcaatatt ttcgagttcc aggtaaagta ctttttcccg
    30001 cgataaaagc agacgcacag agtgctggca tcacattctg tgcgtatggt ttagcctctg
    30061 acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc ggtcataatg
    30121 agcaccgcac cgaaaaattg cagacccgtt aagcgttcat ccaacaatag tgccgcactt
    30181 gccagtccaa ctacaggcac cagtaacgat aacggtgcaa cccgccaggt ttcatagcgt
    30241 cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc cagatacatc
    30301 agagacaaga tggtggtcat atcgatagta accagactgt gaatcatggt tgcggaacca
    30361 tcgagaatca gcgaggcaac aaagaaggga atgattggga ttaatgcgct ccagattacc
    30421 agcgacatca ctgccggacg cgttgcgtgc gacatgatct ttttattgaa gatgttgcca
    30481 cacgcccaac taaatgctgc cgccagggtc aacataaagc cgagcatcgc cacatgctga
    30541 ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc gatccccgcc
    30601 aattgtttgc catgtagtcg ttcaccgaaa gtaaacgcgc caagcacgat agtaaaaaac
    30661 gcttgtgcct gtaacaccaa cgaggccagt ccagcaggca taccaaagtt aatagcgcag
    30721 aacagaaagg caaactgcgc aaaactgatg gttaatccat accccagcag caaattcagt
    30781 ggtactttcg gtcgtgcgac aaaaaagatg gccgggaaag caaccagcat aaagcgcaaa
    30841 ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac aaaatttagc
    30901 ccccatacaa ccactaccag tagcgccaac accccatctt ttcgcgacat tctaccgcct
    30961 ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga
    31021 ttcgttagtc accgtttacg acggcattaa tgcgcaaata agtcgctata cttcggattt
    31081 ttgccatgct aaccatcgga cgcggcgcta cgctgccgtt tatgaccatt tacttgagtc
    31141 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    31201 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    31261 tgttactggc aattaccgcc ttcgctagcg gttttattgc cattccttta gtgaataacg
    31321 tgacgctggt ggtgctcttt tttgccctta ttaactgcgc ctactccgtt tttgctaccg
    31381 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaag attttctcaa
    31441 tcaactacac catgctcaac attggctgga ccatcggtcc gccactcggc acgctgttgg
    31501 tgatgcagag catcaatcta cccttctggc tggcagctat ctgttccgcg ttccccatgc
    31561 ttttcattca aatttgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    31621 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggct
    31681 ttctggcttc ttttgttagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    31741 ttgctgatgg tgattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    31801 tggtggttac gttgcaatat tccgtgggcc gccgccttaa cccggctaac atccgcgcgc
    31861 tgatgacagc aggcaccctc tgtttcgtca tcgggctggt cggttttatt ttttccggca
    31921 acagcctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    31981 caccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    32041 tttccgccca gtctttaggc tggcttggtg ctgcgattaa cccattagtg agtggcatag
    32101 tgcttaccag tctgccgcct ttctcgctgt ttatcatctt agcgttagtg atcgttgttg
    32161 cgtgggtgct gatgttaaaa ggaattcgtg caagaccgtg ggggcagccc gcgctttgtt
    32221 gatttaagtc gaacacaata agaatttaat tcagccttcg tttaggttac ctctgctaat
    32281 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    32341 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    32401 ctgggtttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    32461 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    32521 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    32581 tacgctaaca ggcattttcc tgcactgata acggatcgtt gatacagtag catcagtttt
    32641 ctcaatgaat gttaaacgga gcttaaactc ggttaatcac attttgttcg tcaataaaca
    32701 tgcagcgatt ccttccggtt tgcttaccct catacattgc cctgtccgct cttccaatga
    32761 ccacatccag aggctcttca ggaaatgcac gactcacacc agcagtcacg gtaatgttga
    32821 tatgcccttc agaatgtgtg atggcatggt tatcgactaa ctggcaaatt ctgacacctg
    32881 cacgacatgc ttcttcatca ttagtcgctt tgacaataat gataaattct tcgcccccgt
    32941 agcgataaac cgtttcgtaa tcgcgcgtcc aactggctaa gtaagttgcg agggtgcgta
    33001 atactacatc gccgattaaa tgcccgtagg tatcattaac caatttaaat cggtctatat
    33061 ccaacaacat taaataaaga ttcagaggtt cagcattgcg taactgatga tcgaaggatt
    33121 catcaagaac ccgacgaccc ggcaatcccg tcagaacatc catactgcta cggatcgtca
    33181 gcaaataaat tttgtaatcg gttaatgccg cagtaaaaga aagcaaccct tcctgaaaag
    33241 cgtcgaaatg cgcgtcctgc cagtgatttt caacaatagc cagcattaat tcccgaccac
    33301 agttatgcat atgttgatgg gcagaatcca ttagccgaac gtaaggtaat tcatcgttat
    33361 cgagtggccc cagatgatca atccaccgac caaactggca cagtccataa gaatggttat
    33421 ccgttatttc tggcttactg gcatctctcg cgaccacgct gtgaaacata cttaccagcc
    33481 actggtagtg ggcatcgata gccttattga gatttaacaa gatggcatca atttccgttg
    33541 tcttcttgat cattgccact cctttttcac agttccttgt gcgcgctatt ctaacgagag
    33601 aaaagcaaaa ttacgtcaat attttcatag aaatccggag ttat
//
LOCUS       DAZFRE010000053        33278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFRE010000053 DAZFRE010000000
VERSION     DAZFRE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.053"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..2208
                     /gene="crfC"
                     /locus_tag="ACHKUI_002817"
     CDS             <1..2208
                     /gene="crfC"
                     /locus_tag="ACHKUI_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6598984.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTELPTLI
                     RHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     IAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQDNSESLSE
                     MRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIAQQVKMELSESGFRPG
                     FHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            2205..3083
                     /gene="rdcB"
                     /locus_tag="ACHKUI_002818"
     CDS             2205..3083
                     /gene="rdcB"
                     /locus_tag="ACHKUI_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6598985.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3347..4849
                     /gene="proP"
                     /locus_tag="ACHKUI_002819"
     CDS             3347..4849
                     /gene="proP"
                     /locus_tag="ACHKUI_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6598986.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4961..5050
                     /gene="pmrR"
                     /locus_tag="ACHKUI_002820"
     CDS             4961..5050
                     /gene="pmrR"
                     /locus_tag="ACHKUI_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6598987.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5026..6126)
                     /gene="pmrB"
                     /locus_tag="ACHKUI_002821"
     CDS             complement(5026..6126)
                     /gene="pmrB"
                     /locus_tag="ACHKUI_002821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6598988.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(6127..6795)
                     /gene="basR"
                     /locus_tag="ACHKUI_002822"
     CDS             complement(6127..6795)
                     /gene="basR"
                     /locus_tag="ACHKUI_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6598989.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6792..8435)
                     /gene="eptA"
                     /locus_tag="ACHKUI_002823"
     CDS             complement(6792..8435)
                     /gene="eptA"
                     /locus_tag="ACHKUI_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6598990.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8539..9876)
                     /gene="adiC"
                     /locus_tag="ACHKUI_002824"
     CDS             complement(8539..9876)
                     /gene="adiC"
                     /locus_tag="ACHKUI_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6598991.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10013..10774)
                     /gene="adiY"
                     /locus_tag="ACHKUI_002825"
     CDS             complement(10013..10774)
                     /gene="adiY"
                     /locus_tag="ACHKUI_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6598992.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11110..13377)
                     /gene="adiA"
                     /locus_tag="ACHKUI_002826"
     CDS             complement(11110..13377)
                     /gene="adiA"
                     /locus_tag="ACHKUI_002826"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6598993.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEIQPETLQKKISESPLTKDKVGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13577..14509)
                     /gene="melR"
                     /locus_tag="ACHKUI_002827"
     CDS             complement(13577..14509)
                     /gene="melR"
                     /locus_tag="ACHKUI_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6598994.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTI
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14768..16123
                     /gene="melA"
                     /locus_tag="ACHKUI_002828"
     CDS             14768..16123
                     /gene="melA"
                     /locus_tag="ACHKUI_002828"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6598995.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(16120..16770)
                     /gene="yjdF"
                     /locus_tag="ACHKUI_002829"
     CDS             complement(16120..16770)
                     /gene="yjdF"
                     /locus_tag="ACHKUI_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6598996.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(16893..18539)
                     /gene="fumB"
                     /locus_tag="ACHKUI_002830"
     CDS             complement(16893..18539)
                     /gene="fumB"
                     /locus_tag="ACHKUI_002830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6598997.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQSPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(18617..19957)
                     /gene="dcuB"
                     /locus_tag="ACHKUI_002831"
     CDS             complement(18617..19957)
                     /gene="dcuB"
                     /locus_tag="ACHKUI_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6598998.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(20528..21247)
                     /gene="dcuR"
                     /locus_tag="ACHKUI_002832"
     CDS             complement(20528..21247)
                     /gene="dcuR"
                     /locus_tag="ACHKUI_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6598999.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCRGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(21244..22875)
                     /gene="dcuS"
                     /locus_tag="ACHKUI_002833"
     CDS             complement(21244..22875)
                     /gene="dcuS"
                     /locus_tag="ACHKUI_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6599000.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            23056..23286
                     /gene="yjdI"
                     /locus_tag="ACHKUI_002834"
     CDS             23056..23286
                     /gene="yjdI"
                     /locus_tag="ACHKUI_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6599001.1"
                     /translation="MDRALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            23298..23570
                     /gene="yjdJ"
                     /locus_tag="ACHKUI_002835"
     CDS             23298..23570
                     /gene="yjdJ"
                     /locus_tag="ACHKUI_002835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6599002.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            23799..24095
                     /gene="ghoS"
                     /locus_tag="ACHKUI_002836"
     CDS             23799..24095
                     /gene="ghoS"
                     /locus_tag="ACHKUI_002836"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6599003.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            24123..24296
                     /gene="ghoT"
                     /locus_tag="ACHKUI_002837"
     CDS             24123..24296
                     /gene="ghoT"
                     /locus_tag="ACHKUI_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6599004.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(24415..25932)
                     /gene="lysS"
                     /locus_tag="ACHKUI_002838"
     CDS             complement(24415..25932)
                     /gene="lysS"
                     /locus_tag="ACHKUI_002838"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6599005.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(26169..27626)
                     /gene="dtpC"
                     /locus_tag="ACHKUI_002839"
     CDS             complement(26169..27626)
                     /gene="dtpC"
                     /locus_tag="ACHKUI_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6599006.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(27685..29832)
                     /gene="cadA"
                     /locus_tag="ACHKUI_002840"
     CDS             complement(27685..29832)
                     /gene="cadA"
                     /locus_tag="ACHKUI_002840"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6599007.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(29912..31246)
                     /gene="cadB"
                     /locus_tag="ACHKUI_002841"
     CDS             complement(29912..31246)
                     /gene="cadB"
                     /locus_tag="ACHKUI_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6599008.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(31612..33150)
                     /gene="cadC"
                     /locus_tag="ACHKUI_002842"
     CDS             complement(31612..33150)
                     /gene="cadC"
                     /locus_tag="ACHKUI_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6599009.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         8459 a         7969 c         8380 g         8470 t
ORIGIN      
        1 gagttaagtc aggaggctga acgcctgtta cagctttctc gccaacagtt gcagttactg
       61 gaaaaaatgc ctctctctgt acccggagac gatgcgccac aactggcttt accctggagt
      121 cagcctaata tcgccgaacg tcacgcgatg ctgaataatg agttgcgtaa aatttcccga
      181 ctggaaatgg tgctggcgat tgtcggtact atgaaagcag ggaaatcaac caccattaat
      241 gccattgttg gtacggaggt tctgcctaat cgcaatcgcc caatgactga gctgccgacg
      301 cttattcgcc atacgcccgg gcaaaaagaa ccggtactgc atttttcaca tgtcgcgcca
      361 atcgaccatt taattcaaca attacaacag cgcctgcgtg attgcgatat taagcatctg
      421 accgatgtgc tggaaataga taaagatatg cgtgcgctta tgcagcggat cgaaaatggc
      481 atcgctttcg aaaaatatta tctgggtgct caacctattt ttcattgtct gaaaagtttg
      541 aatgatttgg tgcgactggc gaaggcgctg gacgtcgatt ttcctttttc tgcttacgcc
      601 gccattgagc atattcccgt gattgaagtg gagtttgtcc atctggcggg gctggagagt
      661 tatcccggtc agttgacgtt actggatacc cccgggccaa atgaagccgg gcaaccgcat
      721 ctgcaaaaaa tgcttaacca gcagctggca cgcgcctcgg cggtactggc ggtgctggat
      781 tatacgcaac tgaaatcgat ctccgatgaa gaggtccgtg aggcaatttt ggcggtgggg
      841 caatcggtgc cgttgtatgt gctggtcaat aagttcgatc aacaggatcg taacagtgac
      901 gacgctgacc aggtgcgggc actgatttcc ggaacgctga tgaaaggctg tattacgcca
      961 cagcagatat ttccggtgtc gtcgatgtgg ggctacctgg cgaatcgggc gcgccatgag
     1021 ttagccaaca acggtaagtt acccgcgcca gagcaacaac gctgggtgga agattttgcc
     1081 catgccgcgc tcggcaggcg ctggcgtcat gccgatctgg cggacctcga acatattcgc
     1141 catgctgccg atcagttgtg ggaagattcg ctgttcgccc agccaattca ggcgttgctt
     1201 catgccgctt acgctaacgc ctcgttgtat gctctgcgat ctgccgcgca taaactgttg
     1261 aattacgcgc agcaggcgcg ggaatacctg gattttcgtg cgcacgggtt aaacgtcgct
     1321 tgtgaacaat tgcggcaaaa tatccatcag gtcgaagaaa gtttgcagct attgcaactc
     1381 aatcaggcgc aggtgagcgg cgagattaaa catgaaatcg agttggccct gacctccgcc
     1441 aaccactttc tgcgtcaaca gcaagatgcg gtgaatgcgc agttagccgc gttgtttcag
     1501 gataattcgg agtcgttaag tgagatgcgt acccgctgtg agacactgtt acagacggcg
     1561 cagaacacca tcagtcgcga ctttacgctg cgctttgccg agcttgagtc caccctttgc
     1621 cgggtgttaa ccgatgttat tcgccccatt gcgcaacaag tcaaaatgga attgagtgag
     1681 tctgggtttc gtcctgggtt tcattttcct gtttttcacg gcgtagttcc ccacttcaac
     1741 acccgccagc tgttcagtga agtcatttcg cgccaggaag caacggacga gcagagcacg
     1801 cgtttaggcg ttgtgcgtga gactttttca cgctggttga atcagcccga ctggggacgg
     1861 ggaaatgaga aatctccgac agagacggtt gattacagtg tgttgcaacg agcattaagc
     1921 gcagaagtcg atctttattg ccaacaaatg gctaaagttc tggcagagca ggtcgatgaa
     1981 tctgttacgg caggcatgaa tacttttttc gctgagttcg cttcatgttt gacggaatta
     2041 cagacgcgtt tacgcgaaag tatggctctg cgtcaacaaa atgaatcggt ggtcaggctg
     2101 atgcaacagc aattgcagca gactgtgatg actcacggct ggatttacac cgacgctcag
     2161 ctgttacgcg atgatattca aacacttttc acggcagaac gatattgacc aagacgttac
     2221 tggacggccc cggtcgcgtg ctggagtcgg ttcatccgcg ctttttagta gatctggcgc
     2281 agggcgatga tgcccgcctt ccacaagccc atcagcagca gtttcgtgaa cgactgatgc
     2341 aggaacttct tgcgcgcgtg cagcttcaga catggacgaa cggcggcatg ttaaatgcgc
     2401 cgcttagtct gcgtctgaca ttggtggaga aactggcgtc gatgctggat cccggtcatc
     2461 tggcgctgac gcagatcgcg cagcatctgg cgctgctgca aaaaatggat caccgccagc
     2521 actctgcttt cccggagctc ccccagcaaa ttgtcgcctt gtatgagtgg ttttcagccc
     2581 gttgtcgctg gaaggaaaag gcgttaacgc aacgaggcct attggtgcag gcgggtgaac
     2641 agagcgagca aatttttacc cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg
     2701 ggcgctgttt tatcgttctg gaggagttgc gctggggtgc atttggcgat gcctgccgtc
     2761 tgggaagccc gcaagcggtg gcgttgttgc tgggtgattt gcgcgtgaaa gcgacacaac
     2821 atttggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
     2881 cctcttcgac cgtttcgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
     2941 ggtctacggc agataaacaa cccgtttgct ggtcagtgac ccaacgctgg cgaactgtcg
     3001 cactggggat gccacgactc tgttcagcgc agcgtctggc gggggcaatg gtcgaggaaa
     3061 tcttctctgt aaatttggtg taaataatca gttacaccaa tgagtcctaa acgaaataca
     3121 tgtgtgaagt tgatcacaaa tttaaacact ggtagggtaa aaaggtcatt aactgcccaa
     3181 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agagtgtaag caaacccgct
     3241 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
     3301 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa
     3361 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt gatgatggta aactgcgtaa
     3421 agccattacc gcagcatcac tgggtaatgc aatggaatgg ttcgattttg gtgtttatgg
     3481 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
     3541 ggttgctgca ctcgccactt tctccgttcc ctttctgatt cgaccgcttg gcgggctctt
     3601 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
     3661 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
     3721 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
     3781 taccggggcg tcaatatttg ttgcggaata ctcccctgac cgtaaacgtg gctttatggg
     3841 cagctggctg gactttggtt ctattgccgg gtttgtgctg ggtgctggcg tagtggtgtt
     3901 aatttcgacc attgtcggcg aagagaactt cctcgattgg ggctggcgta ttccgttctt
     3961 tatcgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagagactcc
     4021 ggcgttccag cagcatgtcg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
     4081 cccgaaagtc tcgtttaaag agattgccac caaacactgg cgcagcctgt tgacatgtat
     4141 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
     4201 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
     4261 gatcggtatg ctgtttgttc aaccggtgat gggcttgctg agtgaccgtt ttggccgtcg
     4321 tccgtttgtg cttcttggta gtgttgcact gtttgtgttg gcgatcccgg cgtttattct
     4381 gattaacagt aacgtcatcg gcctgatttt tgccggctta ttgatgctgg cggtgatcct
     4441 taactgcttt acgggcgtta tggcttctac cttgcctgcg atgttcccga cgcatatccg
     4501 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
     4561 gctggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
     4621 ggtagtggcg gtggttggtt taatcaccgg cgtaaccatg aaagagacgg cgaatcgacc
     4681 gttgaaaggt gcgacaccag cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
     4741 gcattacgat aatatcgagc agaaaatcga tgatattgac cacgagattg ccgatttgca
     4801 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
     4861 tggcctgatg tgacgctgtc taatcaggtc aattgaactc ttaaggttca cttaatctct
     4921 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatga
     4981 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
     5041 cacgtactga tcttttttca gccgtatcca ggcccgcgtg ccggaagtct cttgccggtt
     5101 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
     5161 accaatcccg ccataacggc tgtccattcg tacaaacgct ttactcaact ccccgcattt
     5221 actctcatca atacccggtc cttcatcttc aacggccatg accgctccgc catcttcttg
     5281 cagcttaatc ataatgttgc tgccttgtgg gctataacga tgggcgtttt ctaccaggtt
     5341 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
     5401 tggcaatagc agggtttgct ggcgctggtc gagcatggta ctaagttcgt catacgaggg
     5461 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
     5521 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gtcgtgccac
     5581 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
     5641 cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
     5701 cgtgctggtc aggcgactga ccagatcgtt taatgccgaa accaccgcat cgatttcgag
     5761 ggtggcgcta tgaatggcaa tgggcgttaa gttgtcggcg gtgcgcgctt ccagctcttt
     5821 ttgcagctcc gccagcggtc gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
     5881 caggctaacc ataaagacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
     5941 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
     6001 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
     6061 cccgatggtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aacgcatcag
     6121 attcaattag ttttcctcat ttgcgaccag catatagcca aatccgcgca cggtgcggat
     6181 acgggctttg cccactttgt cgcgcaggtt atggatatgc acttccaggg tgttggtcga
     6241 gggctcgttg tcccagttgt agatatcgtt gtagagaatt tcccgatgca ccgggctgcc
     6301 tgctttaagc atcaggcgcg acagcagagc atattctttg ggcgtcagaa tcagttcttc
     6361 tccgctcatc catacctggc ggcgacccat attcagcgtc aggttgccaa cgttcagctc
     6421 actttcgccc tgattattat ggcgtcgtag cagggcgcgg atacgggcgt gcaactcttc
     6481 cagcgcaaaa ggcttcacca gataatcgtc ggccccgaca tccagcccgg cgattttgtc
     6541 ggtaagggta tcgcgggcgg tgagaattaa caccggcaga gtgtattttt tctgccggat
     6601 acgggcaaga aaatgcagcc catcttcatc gggtaagcct aaatccagta ccaccaggct
     6661 gtaatgaccg gcctcaaggc tttgttccgc catccgcgcg gtgctcacgc catcgcacgc
     6721 gtagccttcg gtttgcgccg ccagaatcaa tccctgcaat aacagcgtat cgtcttcaac
     6781 aatcagaatt ttcattcact cactctcctg caagtttgca gaatatcatc cgcagcctgg
     6841 taatacttcg tctcaacgcc agtcaggccc aataaggttg agaataaatt gtcttgtgaa
     6901 tagtgttgcg tttgcgcctg tttttgcagg cagttctggt caacctgata ccgtttttga
     6961 taatcctccg atagccacag cagcatcggc acctgttttt ggctatccgg ggcgatggca
     7021 taaggcagac cgtgcagata gatgccattt tcacctaacg attcaccgtg gtcagaaaga
     7081 taaaccaggc tggtggtaaa tttatcctga tgttctttca gcagattaat cgctttatca
     7141 acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt tcaccagttg ctcttgggta
     7201 caggtctgga tctcgttagt gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag
     7261 cggttgtaat aggtcgggcc gtggctgccg atggtgtgta agacaatcac gccatcaccc
     7321 tgcaggttat tgatgtaatc ttccagcccg tggaacagca cttcatcata gcattcgccg
     7381 ttgatgcact gaccaggcag gttcagcgcg gtgacgttct ggtgaggtac gcgatcgcaa
     7441 acgcctttac agccgccatc gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg
     7501 atatccagca cgccttcctg gtgctgtgcc agctcttctt tgtagtgctc acgcggcata
     7561 tccgagaaca tgcacggcac tgagacggct gttgctgtgc cacaagatgc ggtattaggg
     7621 aaatagacca cgttatcttt tgccaggcga ggattggttt cgcgtggata accattcagc
     7681 gagaaattct ctgcgcgcga cgtttctccg acgatcaaaa tggtcaggtt tttgcgtttc
     7741 ccattttgca ttaatgggtt gcggtgagca tcttcaccaa tgcgtatcag tggcaggttg
     7801 gctgaccgct ggtgagagta ccacgaccag ctagcaacaa tgctgttgga cgggcttaag
     7861 gatttcacca gctctttgtt gttgcgaaac agcgaggcat agtctttgta gaacagtgcg
     7921 gcaaccagca gaatcagcag cacagaaatc aggatgttgg ccccgcggaa aagaacgctg
     7981 cgcaggcgtg aggtggtggg tttgattttg atccagcagg caataatcgc ggctaacacg
     8041 ccacttaacc ccagcgttaa taacattcgt ggcgtcatca acgcataact ttccgccggt
     8101 gtagtatcaa tgatattggt gatcattgaa cggtcgatga cgatgccata ggtcatgatg
     8161 aaatattgtg cagccgcacc gaccagaata aacaggcagg cgagtggacg attaagccat
     8221 aagaaagagc ctagtgtcag gacaatatta atcacgctga atgcgacgac cggcatcgac
     8281 aagaaaacca gcacgttatg tagtgaatcc agcggtaaca cctgtagcac ctgtttaaaa
     8341 aaggcgatat tcaggcagac tgagatataa aaagtagcaa gtaataacca ggagaataaa
     8401 tttaaagagg gtctttttag taggcgcttc aacatcacgg tgtttccatc gaacaaagtg
     8461 cgcatatgct cgcaaagcaa aattaagcca accttaagtt cttaaggttg gcttttatgt
     8521 ttgcttgatt aaggggaatt aatctttgct tattggtgca tctaagggat acgggttttt
     8581 atgcagccgg ttgtaattca gcgcatacat ggcggtgatt accatcagag tgacaaatga
     8641 ccacataacc tctttcgcgc cggaccctac tacggcccag atgcagtaga ggaaggcaat
     8701 ggtagtaact gccaggtatg ccgggcgtgc tttaccaaag tgaccgtggc cgagcagtag
     8761 taacgccgca caggtgtaaa gatatggcac cagtgtaaag atgaccgaca cggaagaaac
     8821 cagaccgaat tctttagtcg cgtttggcga aatgctactg agctggaaga tggtcatcaa
     8881 aataccgacg ataatcagcc ccgccactgg cgtgcccgct ttatttacac gggcaaaaat
     8941 cggtgggaac agtccgtcat cggcagcggc tttcgccgtt tgacccgcca gcaacgtcca
     9001 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccggcggt
     9061 gtcacccaac gccatccgtg cggcatcacc gaatggcgaa gcagaaacgc gcagtgcggc
     9121 attagggatc atccccataa tcgcggtggt agaaagtaca tagcaaacgg cagcaatcaa
     9181 taccccgcca atggtggcga tagggacgtt gcgtttcggg tttttcacca cacctgcggc
     9241 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
     9301 cgcaccgaag gtgcccagac cgctgacgtt ccatgccgcc atataggttt caccacggaa
     9361 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cggtagcaac
     9421 ggcctgtacg cgggtgatca ttttcggacc gacaatgttc agcaggacga agatccacag
     9481 caccacgacg caggtgatgg ttaataccaa tgggtctttc agaatcggga agaagtaact
     9541 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
     9601 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
     9661 accaccagga cttgggtcga ggaacgacat tttggcgtat accatcgaga gccccagcgc
     9721 accgataatc gtcaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
     9781 aggtaacaga aaaacacctg accccataat attccccgac accatcaggg tgacggggat
     9841 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
     9901 ttcgtgacaa taaattacgt cgtatcatac gcctgcattc gatagttgaa ttattatcgg
     9961 ccgggttatc gctgatgccg ttattaatat agctcttcgt taaggttaaa aatcaggcga
    10021 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
    10081 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
    10141 ttttaccgtc catcattaat tcatttacgg catagcgcat gcggcaggtg gtgattattt
    10201 ggctataact ggtgttttcg cttttcagct ttttctttaa cagacttggg ctaagacata
    10261 aacaactggc taccatacta agattccagt ctttgtggat atcgctttca ataatttgat
    10321 aaacgctgtc acttacacaa ttacgtaaca tatacatcat tagtgaaagg aattttttac
    10381 tgtcgagaaa acgggatagc acagtgaata ataatgcacg tgttcgttcc ttttctgact
    10441 cagtttcagc gggcatcatg ctatgctgcg cggcaacgcg aaagacgtct ggcgtcaggc
    10501 atggcagggt gaggatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
    10561 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
    10621 tattaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atgtggttag
    10681 ctttaaggct aataggttct ttcccattca cccttatcca gacatctttt tcagtcaata
    10741 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
    10801 gaccagttct cgctaaagcg aagcgatacc gggaaatggt tatataattg tatgatgaat
    10861 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
    10921 atgtacatat ttttttacag gctaaaaaaa ggataaataa caggcaaaaa aaagcgcggg
    10981 gtgggaccgc gcaaaaagca tgacattttt tgtttgcagc aatttttatg aaaacgttgc
    11041 catacttaaa aggcctcaag ccaggcggcg taatgttatt taacaattac gccttcagcg
    11101 gaatagtggt tacgctttca cacacataac gtggtaaata ccgtcaataa tttctgtccc
    11161 ttctgtttcg tgttcaaatc cagggaaatg atggtcccag gattgcagtg agcgtaaata
    11221 acttacttgc ggactgtttt tatcgccgaa gttttcacca gacagcagca tcgggattcc
    11281 tggcggatac gggataactg agtttgccgc gatacgtcct ggcagatttt caatggatac
    11341 cagttcgaca ttgttgtcga caatcgcgtt gtacgcttca cgcggggtga tttccgccac
    11401 cggtaggcca gaataggctt cattcaaccg tgcgccaggg ttgttttctt tcagccaggc
    11461 aaacatggtg tcacccagat cgtgaatccc catgttcgcg taagtgtcag gatattgttc
    11521 aacaagttcc ggcatcacct gcgccagcgg tgtgttggca tcatagtggc gtttgaagga
    11581 gcaaagggtg ttaaccagag tcccccattt cccacgggtc acgcccatag agaacaggaa
    11641 cataatctgg aagtcagtgg tacgggtagg cacaatgccg tggcgaccaa gccaggctgt
    11701 gaccagcgcc gccggaacac cggtttcttc cagttcacca tcttcaccca ttcccggagc
    11761 aaggatgctg actttaatcg ggtcgagcat actccagtta tccggaatat ctttgaagcc
    11821 gtgccagctt tcgcccggat gcatgaccca gcagtcctga acggtggtca gcagtttggt
    11881 tggtgcgtca gcaaagtcat aggttttgcc ggtttgtggg tcggtgacga cttctttgtt
    11941 ccatggtttg aagaaccagc taccgtcagc ggtgaactct ttatatagcc gcgccatcgc
    12001 ctgacggaaa tcaaccgctt cgtcgatcac ttcctgggtc agtgacaggc cgctgttgcc
    12061 gtccatcatc gacactgcca cgtcgttgga tgcgcagatg gcatacagcg gggaggtggt
    12121 ggcatgcatc atgtaggcct ggttgaagcg ggagaagtta atcgccccac gaccttcacg
    12181 tacatgaata taagaggcct gggacagcgc attcagcagt ttgtgggtgg agtgggtggc
    12241 gaaaacggta ggaccgttgt gatcgccagg ttcgccgcgc atggcatagt gatcggcata
    12301 gatcgggttg aaacgtgcat agccgtacca ggcttcgtca aagtgcagac gatcggaggt
    12361 tttttccagc agatcctgcg cttctttagc gttataacac acgccgtcat aggtacagtt
    12421 ggtcaccacg cagtaagacg gtttttgccc gactttgtct ttggtcagcg ggctttcact
    12481 gattttcttc tgcaaggttt caggttgtat ttcctgcgga tagattggcc cgataatgcc
    12541 gtagcggttg cggcttggca ccatgtagac cggtttcgcg cctgtcagca tcaagccttg
    12601 ttcgatggat ttatggcagt tacggtcaac gaccacgaca tcgttatcgg tcatgcaagc
    12661 ctgcatgatg gtgcggttag agccggaagt accgacgact accgaccagg agcgatcggc
    12721 accaaatacg cgtgcggcat atctttcgct ttcgccaaat gcgccagtat ggtcaagcaa
    12781 agaaccgagg gaagtgcgtt cgatgcccat gtcggtgcgg aacagatttt caccataata
    12841 gtcatggtag aaacgtccgg cgggtgtttt ggtaaaacca acgccgccct ggtggcctgg
    12901 cgctgcccag gaatattcat ggatgtcgct atatttcatc agcgcgctga acagtggcgg
    12961 caatagctgc tggcggtagc gggtcatcgc ggcaacggcg cgtccggcga taaagtcggc
    13021 ggtatcttcc agaatccagg cgaattcatc gacaagctcc agcagatccc ggtccattgc
    13081 ggcgagggct ttttcccgat cgcccaacag gaagaccggc acgttttgtt ggcgctcatg
    13141 aagcttaccg atcaattgtc tgacgttttg atgttcgtcc ggatgttcca tttgatagct
    13201 gaacatcagg cagtcaatgg cttcgtttga agagagaatg gcaaaaccat catcaaagga
    13261 ggtggattta atcacggtaa cattttgctg gcttaaagca tcagccagac gctcaacggc
    13321 gttaccgacc caggtgtctt gatggagaaa ctcgctttca acaattaata ctttcatcat
    13381 tgcttacccg gttatgaagg aatcttcgtt gcgggcaagt atcttccggc cttttcgggc
    13441 tgtaaagcgc gtgaaaaaca aacatttttg aaatatttgt tttttataaa caatatgtta
    13501 cgtgattatt ttaaattatt ttcactgctt attatcgacg gctaaactat ttttttggct
    13561 gatactgata tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt
    13621 attgttgcgg tgacatgccg acatatttgc cgaacgtgct gtaaaaacgg ctactcgaac
    13681 gaaagcctgc cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc
    13741 gaacgtggtt gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct
    13801 ggaatatccc cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg
    13861 tcagcgcctg atcatagttt tcggcaataa agcccagcat ctggctgaca taaaattgcg
    13921 catggcgcga gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc
    13981 agccagagag gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa
    14041 tttgctcgtt cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt
    14101 gctgtgtcgc cagtgatttg atcaccatgc cgtgagtgac gtggttaatc aggtctttat
    14161 ccagcggcca ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg
    14221 ttccggtatc tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat
    14281 tgatattcac tttttcattg ttgatcaggt attccacatc gccatcgaaa ggcacattca
    14341 cttcgacctg accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaattt
    14401 ccatccgctg gtattctgaa tacagcgaca gtgggctacg ggtctgcttt tcgtcgctgc
    14461 tgcacataaa cgtatctgta ttcatggatg actctctttc ctggaatatc agaattatgg
    14521 caggagtgag ggaggatgac tgcgaacagg cgcacggttt tattcctctc cctggaggtg
    14581 cgaggagagc ggatgagaag atttgggtcg attattcagc ttttcaggta atttattccc
    14641 ataaaatcag atctattgat gtgccactta ggatctgagt ttttgggaat actcaaccgg
    14701 gaaaccggag gttttctgcg atatcgcctg ccatgatgaa gtcattcaag caagccagga
    14761 gatctgcatg atgtctgcac ctaaaattac atttatcggc gctggttcga cgattttcgt
    14821 taaaaatatt cttggtgatg tgttccatcg tgaggcgctg aaaacggcgc atattgccct
    14881 gatggatatc gatcccaccc gcctggaaga gtcgcacatt gtggtgcgta agctgatgga
    14941 ttcagcaggg gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga
    15001 tgctgatttt gtggtggtgg catttcagat tggcggttat gaaccttgca cggtgactga
    15061 tttcgaggtc tgtaagcggc atggtctgga acaaaccatt gccgatacgc tggggccggg
    15121 cggcattatg cgtgcgctac gtaccattcc gcacctgtgg cagatttgcg aggacatgac
    15181 ggaagtctgc cccgatgcca ccatgctcaa ctacgttaat ccaatggcga tgaatacctg
    15241 ggcgatgtat gcccgctatc cgcatatcaa gcaggtaggg ctgtgccatt cggtgcaggg
    15301 aacggcggaa gagctggcgc gtgacctcaa tatcgacccg gctacgctgc gttaccgttg
    15361 cgcaggtatc aaccatatgg cgttttacct ggaactggag cgcaaaaccg ccgacggcag
    15421 ttacgtgaat ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc
    15481 aaatattcat ggtaatactc gctgccagaa tattgtgcgt tacgaaatgt tcaaaaagct
    15541 gggctacttc gtcacagaat cgtcagaaca ttttgctgag tacacaccgt ggtttattaa
    15601 gccaggtcgt gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg
    15661 ctgcgtcgag cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg
    15721 gattgatatt aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg
    15781 cgagccgagt gtgatttatg gcaatgtccg taacgatggc ttgattgata acctgccaca
    15841 aggatgttgc gtggaagtgg cctgtctggt tgatgctaat ggcattcagc cgaccaaagc
    15901 cggtacgcta ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct
    15961 gctgacagaa gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga
    16021 cccgcatact gccgccgtgc tgggcattga cgaaatatat gctcttgttg acgacctgat
    16081 tgccgcccac ggcgactggc tgccaggctg gttgcaccgt taaggggtaa tcgcttccgg
    16141 cgctctggtg attaagccaa agcgccgtag ctggcggcaa tgaaagcggg cgaggaatat
    16201 caccgtcgtt aatgcgccca gtagtgcgca gaacatatcg gattgcgtat cccacgggtc
    16261 gccttgagta ccgagaaaat catctgcgcc ctggcccatt gccagcgctg cccaccactc
    16321 gattaactca tacatcgcgc ttatcgccag cgcgacacaa cagaccagaa acgccaccat
    16381 tttacgcccg cgaacgtaca tcccgcgcac gaggatctct cgtgccacca gtgcaggcac
    16441 cagcccctgg aaaaaatgcc ccagtttatc atacggatta cggctcaacc ccagccattc
    16501 ctgcacctca aaaccaacgg ggactttcgc gtaggtgtat tgcccgccga ccatcaggat
    16561 gatggcgtga aagaaaatga gcgtatagag cagcggcgtt aacgggtaac gtttagcggt
    16621 ggcaagcagt agcggcacga caataatcac cggtgtcact tccatcagcc aggtgagttt
    16681 gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt
    16741 aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga
    16801 ctattgacca gtaagcgtga aattacaatc gcaaaagagg tggcccaggg ggatcacctg
    16861 gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
    16921 ttgacgatct gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa
    16981 tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
    17041 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag
    17101 aagccgccgt gtttatggca cgcgtcggta acctgctggc tgcggttacc tttcgccagc
    17161 atgatcatgc tgccgccgtg ggattgcagc agatccacgt aggagtccat acgacctgcg
    17221 gtggttgggc caagtgaacc tgatggataa ccggcaggcg ttttcgccgg acccgcgtag
    17281 tagatcgggt gatctttgat gtactgcggc agttctttac cggcgtcaat cagctctttc
    17341 agcttggcgt gtgcaatatc gcgacccacg ataatggtgc cggtgagcga caagcgggtg
    17401 gataccgggg attgcgaaag ctgggcaagg atctctttca tcgggcggtt aaggtcgact
    17461 ttcactgctt cgccttcacc tgcctgacgc agttcttctg gaatgtactg gcctggattg
    17521 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca
    17581 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
    17641 acgcggatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
    17701 gcctcttcca gcagttcctg ttccagctgg atatcgcgga acgcctgacc atgttcgttc
    17761 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta acttgacggt tttcaggttg
    17821 gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta
    17881 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggcgt cagcagggct
    17941 ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttagc gacacagagg
    18001 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttcgtgccg
    18061 gtgttgacct ctttatacat gtccagcggc gcgttctgtg aatagcgcag gttatcttcg
    18121 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
    18181 cgctgacctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg
    18241 cctttagcgg cgatttcgga gtttcttaag aattgcagcg ccacgtactt gtcgttttcg
    18301 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg gcggagcatg
    18361 aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
    18421 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cagaagtgag tagatagtat
    18481 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
    18541 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc
    18601 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
    18661 acgatacgct cacaccaatc aaccccggca gaataaagct gtggttgatg acgaagcgac
    18721 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
    18781 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc
    18841 cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
    18901 tggaaaccag cagcagaaca atggcatagg cccacggata ctctttcacc atttcaccca
    18961 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttcagccatc catgcgatac
    19021 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
    19081 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
    19141 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
    19201 caagaagggc gactacagcg attgccccga ggaagatcca cattgccagc cagttgcttt
    19261 tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
    19321 gtacggagat gaatttttgg aactcttcgt ctttatccag atctttaccg cggaaccagc
    19381 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcaggt
    19441 cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcatcgca accaaagata
    19501 cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
    19561 ccatcggacg ttccggacgg atgttgttct taatagcaac gtcgtagatg atcggcagaa
    19621 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag cgtacaggtc acaaacggcg
    19681 cgacaattga gacatatttc gggttgcgac gcagcagctt ctcggcaatt tgcagcatga
    19741 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
    19801 gcatgacatc aaccggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca
    19861 gaccgatacc gcctaataaa cccagcgcga tacccccctt tctggcacca taaaacagac
    19921 atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
    19981 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
    20041 gcctgactaa atatccgttt ggtcgttacc ggcttgagca aatacctcac agtgaatatt
    20101 ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaag
    20161 actctctggc gatgggttta tagtttgtac agcgcagatg ttaatgtttt gtttgctgtt
    20221 atcttattga tatttatcgg tctgtttttt gttctcaggg caggtatcat taggtttata
    20281 gattaatctg atctacccat ttgtgggtaa aaatacacat aatgtgggtg acataatagt
    20341 taattaactt ttgttagcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat
    20401 ttttagcgat gatggaggga taattatatt tgatctggca caagttttac tgatgaacga
    20461 tgtaacttgt gccaggggga ttttgcatta cggtaataat tattacttac agataaccag
    20521 cgatccgtta ttggcaatat tgtttcagta gtgagtagtg ttctgcctga atacggtaac
    20581 ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaatatg tggcagttga
    20641 ccagccagat aaggtattta cggcaggaaa cacgcgagat gttaacctcg ttagccagct
    20701 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
    20761 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ctcgttggag ctgctgccgt
    20821 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
    20881 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
    20941 aatccacaac accgtaatgc agcgaatctt taatggttac tgcatcggca gcggaggaga
    21001 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
    21061 tctctttttg catatagata tctagcaata tcaggtcgat aggcgtatcg ctattgaaga
    21121 taatctcttt ggctttctcc agcgtcgaag ccgttccacg gcattgaaag cccgggattt
    21181 gtgccacgta tcggcgattc agctccgcga ccattgcgtc atcatcgata attaatacat
    21241 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
    21301 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
    21361 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
    21421 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
    21481 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cctccagcgc
    21541 gttttctatc agatttccca acgtggtaat cagcgtcgcg acctgatcct cactaccgct
    21601 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
    21661 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
    21721 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
    21781 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
    21841 acgaagtgcg tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgtacttc
    21901 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
    21961 ggtgttgatc agtaatagtc ggtccttaat cgtaatctct tcgtcgcggc gtggggttcc
    22021 gtcgcgtaac acttccgaga catctaccac ctgtgaccac gagtggctta gcgtcgacag
    22081 tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
    22141 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatggact gcaacatggc
    22201 ctggcgttgc tcaaacagtg tggagatttc gtagggttcc aggccgaaaa ggattttttt
    22261 cagtacgtta accagaatgc atgtgccgat cagtccgacc agcatgccaa atagtaccga
    22321 ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta actcaaggcc
    22381 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
    22441 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag
    22501 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
    22561 gctttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
    22621 gatggcctgg attccactct cctgcggttt tttctgcaag ccctgacgga tttccggcga
    22681 gtcggcgagg gtacgcgcta ctgccagtgc cttgttagct agcccgtctc gcgtcatatc
    22741 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
    22801 gaccattaag atcacagtgg tactcaattt catcggacgt ttgcgtaaca tacggtaggg
    22861 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
    22921 gcgggtaatt caaagggagt aagaatgatt ggctatatag gggaagagac tctggcaacg
    22981 gaaactgcca gtgctgtatg aagattccgg ggctatgctt atagcgataa tcatactgat
    23041 gagagaggga aggtcatgga tcgggcgcta ctggacgggg gttatcgctg ttataccggc
    23101 gaaaatatcg atgtctattt taacactgcg atatgtcagc attctggcaa ttgcgtacgt
    23161 ggcaacggca agttatttaa tctcaaacga aagccgtgga tcatgccgga tgaagtcgac
    23221 gttgccactg tggttaaagt gattgatacg tgcccgagtg gcgcgctgaa ataccgtcat
    23281 aaataagcga gggtaaaatg gaaatacgcg aaggccacaa taaattttac attaatgacg
    23341 aacaaggcaa gcaaatcgct gaaattgtct ttgtgccgac cggagagaat ttagcgatta
    23401 tcgaacatac cgatgtcgat gaaagcctga aagggcaagg gattggtaaa cagctggttg
    23461 cgaaagtcgt ggaaaaaatg cgtcgggaaa aacgaaaaat tatcccacta tgcccatttg
    23521 cgaaacacga atttgataaa acacgggagt atgatgatat tcgcagttga tgggagagta
    23581 cagagtcacg atatttttca tgctctccgc ggtgtgatgc aggagagcat ctgaagggta
    23641 gggggatgca caaagaatgg tcagagagag cgtttttttg tcccaagtca tcccctttac
    23701 tgagcaaaaa aaaaagaata tctcctatat gagaatcatc attcggggtt aataagtttt
    23761 gcgtccccag agcgttcaat attgatagga gtcatattat ggaaggtaaa aacaagttca
    23821 atacttatgt tgtttctttt gattatccat catcttattc ctcagtgttc ttaaggttaa
    23881 gatcattgat gtatgatatg aatttctcct ctatcgtggc tgatgaatat gggataccac
    23941 gacaactgaa tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag
    24001 atttaatcag gctcaaatgc ttagacttac cggatattga ttttgacctc agcattatga
    24061 cagttgatga ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa
    24121 aaatggcact attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg
    24181 tcattatctg gtttatctca catgagaaaa cacatattcg tttacttagt gcatttctgg
    24241 tcggaataac ctggccaatg agtcttcctg tggcattact tttttctctc ttttaggagc
    24301 tgtagttgag tggacccgct ggttatcttg aaacgaagcg tctctcctca aaactggatt
    24361 tgcggaatga cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt
    24421 ctgtgggcgc atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag
    24481 cataatcatt cggtcgatac caatacccag accagcggtt ggcggcagac catattccag
    24541 cgcagtcacg taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt
    24601 aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt
    24661 accaatttca cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc
    24721 attacggcgc gccagcgggg acacttctgc cggatattcc gtaataaagg ttggctgaat
    24781 cagatgtgct tctgccactt catcaaagat ctccgtaaca atacgtccca gcccccagct
    24841 tttctctacc gtaataccga tagattcagc taatgcttta gcagcatcaa aattatccag
    24901 atccgccata tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc
    24961 aaacggtttg ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagtac
    25021 ctcttgtgcc agggtgcgga acagtgactc cgtcagttca atcaaatcgt ggtaatccgc
    25081 atatgccatg tagagttcca tcattgtgaa ctcaggatta tggcgaacag aaataccttc
    25141 attacggaag ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt
    25201 cagatacagc tccggcgcga tacgcaggta catatctaaa tccagagcgt tatgatgggt
    25261 aataaacggg cgagcagatg ccccacctgg aattacctgc atcatcgggg tttctacttc
    25321 cataaagccg cgcgcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac
    25381 aaacgtttga cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac
    25441 ttcctgatcc tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag
    25501 gcgcagctca gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg
    25561 ggcaccgata atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg
    25621 caggctatct cttgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa
    25681 ggaggctttc cccataatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt
    25741 taaggattcc agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt
    25801 atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt
    25861 ttcgcggcga tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc
    25921 tcgttcagac attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca
    25981 gatcggtaca ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact
    26041 aacgcttacc tcaagttgat atcactaata aataaccatc aaaatggttt aaatgactta
    26101 ttaatctcga tttactagtg tgataaactg aaagttaatt tattcataac caattgtttt
    26161 tacgacagtt aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg
    26221 gtagcaaagg ccaataccac gatgatcgcg acacaagcca acgtccactc tcccatctga
    26281 gaaaagaatc gctggtaagc ggcaattgcc ataccgctaa tttgcgactc tgtcgtctgc
    26341 tgtgccacga cgcctgccag ccagttggcg actgcgcctg ttgccagcat ataaatacca
    26401 gttaatacgc cagacatttt cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag
    26461 agttcagcaa agcccattag cgccagcccg gatatcatca cgcccattga cgcttgaccg
    26521 ccagccgctg catgtcgggc atcaaatgcc agcaacataa agccacaagc catcagcagt
    26581 aagccaaagg caaacttcag ccagacgcgc aatgctgaat tgccgcggct ttctggcgac
    26641 gccagccagg ccagaacaac cccagcgagc atcaccgcaa tagcattcac cgactggaat
    26701 agtgctgtag gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag
    26761 ctgatggtac tgccgccctg ttgtgccagt acccagaaca acgtcccgac aaacatcaac
    26821 aatacaattt gccaaagagc acggcggtgt tcggggaatt tgatcatcat gcgggcaatg
    26881 atttgtgcgg caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc
    26941 agcagggtaa aaaatactgg ggctaaacag agcatcacca ctaaccagct ccatactggt
    27001 aaggcaaatt tcacgctagt gagcgctttc ttatccatac tacgcgtgga ctggaaatga
    27061 cgatgaccac ttaagaaaat caacaaaccg ataaacatgc cgccacccgc aagggcaaag
    27121 ccaacatgcc atccatacca ctgagcagcc aggccgcagg cgatgggcgc ggcgatagaa
    27181 ccgatattgc ccgcagcata cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg
    27241 tcgtagagct cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata
    27301 atgattgcca gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca
    27361 tggccaaggg tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg
    27421 tcggcaagcc agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac
    27481 aggctgatgg catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag
    27541 gcacgcatgc cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc
    27601 gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca
    27661 agccacttcc cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt
    27721 atagcggcca tcagcctgac ggtatgcacc gtgaatatcg gtttcaaagc ccggatagtg
    27781 agcgccgatt tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat
    27841 catttcaccc ggcattacca gaggaactcc cggcggatac ggaaggatca tattggcgtt
    27901 aatacgacct accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg
    27961 gaacgcagca tacggagtca ttaccatagt cggcagaact tcaaatgcgc gatacatcag
    28021 atccggcaga ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg
    28081 catgttttca tagaattcag gatcttcacg atacagagac ggcagcatgt ttttcacacg
    28141 caggttcaga tcgaacgcac gtttgaagtc agtcagagca cgcagcaggc tcagtgcttt
    28201 ggtcttatcg ataccgatgc tgaacaggaa cagcaggtta tacggaccgg ttttctcaac
    28261 aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg gccggaatac caaagtcgct
    28321 catggtgccg tctttttcca tccccggagt cagcagggtg actttgatcg ggtcaagata
    28381 catgtgctcg ttatcgatgt ttttgaagcc gtgccaggtg ctgtcagaac gcagcggcca
    28441 gcattcagtc gtatcgatat gatccggctg ccatacatca aagaaccagc catcagattc
    28501 cgttctcaga cgtttgatct ctttacggaa tttgatcgca cgttcaatgg aaccattgat
    28561 cagacgttta cctgcattgc ctttcatcat cgccgcagcg gtttcagtgg acgccacgat
    28621 accgtagtgc ggagaggtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt
    28681 tacgtcacct ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt
    28741 agactgggtt tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc
    28801 ttcgtaaatc ggtgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt
    28861 cacatccagt gttttcttga tgaagtcggt gttgtacagc agaccatcat aggtagagtt
    28921 ggtaattaca gcatgtaccg gccaggttgc gtttggtgtt tctttcacgc gcttagcaat
    28981 ggtagcgtgc tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt
    29041 cgggcggaaa tagattggcg taacatcgct catcatcatc aggtgggtca gcgatttgtg
    29101 gcagttacgg tcaatcagaa tggtgctgcc tgccggagca gagtacatac caacaatttt
    29161 gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc
    29221 gatatactgt tctgcttctt tgtgtggacc actgtgatcc agcagagaac ccaattcaga
    29281 tactgaaatg gaaatatcag atttcatggt gttcggacca aagaaatcat agaacaggct
    29341 acctaccggg cttttctgga atgcagtacc acccatgtga ccaggagtac agaaagtata
    29401 tttaccttca cgaacatatt taaacagtgc tttggtcagc ggaggcagaa tagtgttgat
    29461 atattcgtca gtggtctgct tgatcttgtt agcaatatca tcagcagcac ccagcgcata
    29521 ttcaaagaag ctaatctgta aacgcaggtc attcaggctt acatcgagag tggaatacgt
    29581 attagcgaac gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc
    29641 gagattatat ttatcccagt caaaaataac gccgcacaga cgcgcattgt tttcgatcag
    29701 ttttaataag tcgtcacggt cgttcgggta aacaatctgg aagttcagac gttcaagcgc
    29761 gcgatgaagt tcacggatgg gttcttcttt aaaataaacc cccatgtgat tcaatattgc
    29821 aataacgttc atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa
    29881 tgaaaggagg agcctcggaa aatactttta attaatgtgc gttagacgct gtgtggttat
    29941 ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc
    30001 tgacgatgaa ggtacctgcc agctcgaagg agcttgcgcc catcagcgcg atgaagcaga
    30061 acacgcaacc cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa
    30121 aacgaatcag gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac
    30181 cggtcagttc accgaacagg tcagaggctt taccaccggc agagttcatc agagtgatga
    30241 ggatcatcag ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt
    30301 tgctgtcgac ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg
    30361 cctggcctac caacatcatc caggagccca gagaagtcag gcacgcaaag gcggtgaatg
    30421 cagaaaccag tggtgcagcc cagttaccga ggatagttga agcactgatt gcaaacggag
    30481 caccggaagc cgccattaca gaagacggat acataccgga aagcacctga gtcgcagcga
    30541 tgtaaacaat acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg
    30601 ggtttttaac cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc
    30661 agagcagaat acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag
    30721 ctgcataagt tgctgcatca aaccaatgcc agccaacaat agcagtcatc accacaggaa
    30781 taagaaccag caccagacca atcgtggtta aacggcttac ccaggtaccg ccgagcatat
    30841 tcacaaaggt aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat
    30901 ttaatactgg gaagaaggtg gaaagataag atacagcggt aataccaatt gccaggttac
    30961 caatccagtt agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggggaaattt
    31021 ctccggcata cgcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata
    31081 catatgccag cgacattgca ccaataatag agataatcca accccagata gcaataccac
    31141 cgatacttgc taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa
    31201 caacaccggt acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat
    31261 ttcatttttg aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac
    31321 tggcgctaag attactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa
    31381 aagtttctgt aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata
    31441 tcatgaaaag ataaacattc aatgtttaca atgcattgcg ttacattctc tggttaaatt
    31501 tatgtaataa aaattatgcg gcaaataaat tgccgcaaca tattatacca acgggaacat
    31561 acaaaaactc aacaacaagt attcccgagc atgaatcaac ccgggagtta cttattctga
    31621 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
    31681 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
    31741 agctgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
    31801 ccaggacatt tcaagatcaa tgccggtatt tatcgcctga taagactcat ctgttttacc
    31861 ttttaccagg gcactgaccg cttttatttg atatataatg gacaggttgt tcaattccgg
    31921 cagtgtaaca atgttatcta tttctgtgtt cagtgctgct aattgttttt cgtctaaagg
    31981 atgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
    32041 tggggatgat tgaacaatct cacctaataa ttcactggca cgatccaatg atttatcatc
    32101 accatgcagt aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgcgg
    32161 caaaatttgc tggagcatct cctgcattcg ttgtggccac ggttggttta acgcttttga
    32221 taaactctgg agcaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
    32281 tttatccagc atggttgaac catctgcatt gtctaccaat tttaccgaca taaagcattg
    32341 ttgagcacgg tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct
    32401 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
    32461 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gagaactcca
    32521 gctgttacaa ctcttattaa ccatattaat gtcaatatcg cgtggattga gcaaaatgcg
    32581 cgatttactc ataggaagac gtgtttcaag acttgaaaac gctaccagtg cgacacagat
    32641 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
    32701 tggggattgt tcaggtggcg ttgtggtgtt ttgaatgtta agactgtggg agggagaatc
    32761 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
    32821 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
    32881 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
    32941 ctgcgtcaca acgtgattgg tgacaatact tctcttccag acattatcga taagttcatc
    33001 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcga ttaatctcgg
    33061 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
    33121 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg
    33181 caatgataat tgccataacc tattttttcc atctatagat gggtttattt acatattatt
    33241 ggtgaatgta ggacgttatt tttacccgcc ataaactt
//
LOCUS       DAZFRE010000054        32886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFRE010000054 DAZFRE010000000
VERSION     DAZFRE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.054"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            970..1317
                     /locus_tag="ACHKUI_002843"
                     /pseudo
     CDS             970..1317
                     /locus_tag="ACHKUI_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1370..1765
                     /gene="yecN"
                     /locus_tag="ACHKUI_002844"
     CDS             1370..1765
                     /gene="yecN"
                     /locus_tag="ACHKUI_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6599010.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1806..2549
                     /gene="cmoA"
                     /locus_tag="ACHKUI_002845"
     CDS             1806..2549
                     /gene="cmoA"
                     /locus_tag="ACHKUI_002845"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6599011.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2546..3517
                     /gene="cmoB"
                     /locus_tag="ACHKUI_002846"
     CDS             2546..3517
                     /gene="cmoB"
                     /locus_tag="ACHKUI_002846"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6599012.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(3682..6110)
                     /gene="torZ"
                     /locus_tag="ACHKUI_002847"
                     /pseudo
     CDS             complement(3682..6110)
                     /gene="torZ"
                     /locus_tag="ACHKUI_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(6135..7235)
                     /gene="torY"
                     /locus_tag="ACHKUI_002848"
     CDS             complement(6135..7235)
                     /gene="torY"
                     /locus_tag="ACHKUI_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6599013.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGVLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDGTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7623..8369)
                     /gene="cutC"
                     /locus_tag="ACHKUI_002849"
     CDS             complement(7623..8369)
                     /gene="cutC"
                     /locus_tag="ACHKUI_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6599014.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(8383..8949)
                     /gene="yecM"
                     /locus_tag="ACHKUI_002850"
     CDS             complement(8383..8949)
                     /gene="yecM"
                     /locus_tag="ACHKUI_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6599015.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9165..10898
                     /gene="argS"
                     /locus_tag="ACHKUI_002851"
     CDS             9165..10898
                     /gene="argS"
                     /locus_tag="ACHKUI_002851"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6599016.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10951..11343)
                     /gene="flhe"
                     /locus_tag="ACHKUI_002852"
     CDS             complement(10951..11343)
                     /gene="flhe"
                     /locus_tag="ACHKUI_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6599017.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(11343..13421)
                     /gene="flhA"
                     /locus_tag="ACHKUI_002853"
     CDS             complement(11343..13421)
                     /gene="flhA"
                     /locus_tag="ACHKUI_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6599018.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(13414..14562)
                     /gene="flhB"
                     /locus_tag="ACHKUI_002854"
     CDS             complement(13414..14562)
                     /gene="flhB"
                     /locus_tag="ACHKUI_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6599019.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(14771..15415)
                     /gene="cheZ"
                     /locus_tag="ACHKUI_002855"
     CDS             complement(14771..15415)
                     /gene="cheZ"
                     /locus_tag="ACHKUI_002855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6599020.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(15426..15815)
                     /gene="cheY"
                     /locus_tag="ACHKUI_002856"
     CDS             complement(15426..15815)
                     /gene="cheY"
                     /locus_tag="ACHKUI_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6599021.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(15830..16879)
                     /gene="cheB"
                     /locus_tag="ACHKUI_002857"
     CDS             complement(15830..16879)
                     /gene="cheB"
                     /locus_tag="ACHKUI_002857"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6599022.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(16882..17742)
                     /gene="cheR"
                     /locus_tag="ACHKUI_002858"
     CDS             complement(16882..17742)
                     /gene="cheR"
                     /locus_tag="ACHKUI_002858"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6599023.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQKILRRFVPLLKPDGLLFAGHSENFSHLERRFMLR
                     GQTVYALSKD"
     gene            complement(17857..17987)
                     /locus_tag="ACHKUI_002859"
                     /pseudo
     CDS             complement(17857..17987)
                     /locus_tag="ACHKUI_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(18033..19694)
                     /gene="tar"
                     /locus_tag="ACHKUI_002860"
     CDS             complement(18033..19694)
                     /gene="tar"
                     /locus_tag="ACHKUI_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6599024.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(19839..20342)
                     /gene="cheW"
                     /locus_tag="ACHKUI_002861"
     CDS             complement(19839..20342)
                     /gene="cheW"
                     /locus_tag="ACHKUI_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6599025.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(20363..22327)
                     /gene="cheA"
                     /locus_tag="ACHKUI_002862"
     CDS             complement(20363..22327)
                     /gene="cheA"
                     /locus_tag="ACHKUI_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6599026.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     GQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGDERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(22332..23258)
                     /gene="motB"
                     /locus_tag="ACHKUI_002863"
     CDS             complement(22332..23258)
                     /gene="motB"
                     /locus_tag="ACHKUI_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6599027.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAINRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(23255..24142)
                     /gene="motA"
                     /locus_tag="ACHKUI_002864"
     CDS             complement(23255..24142)
                     /gene="motA"
                     /locus_tag="ACHKUI_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6599028.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(24269..24847)
                     /gene="flhC"
                     /locus_tag="ACHKUI_002865"
     CDS             complement(24269..24847)
                     /gene="flhC"
                     /locus_tag="ACHKUI_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6599029.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(24850..25200)
                     /gene="flhD"
                     /locus_tag="ACHKUI_002866"
     CDS             complement(24850..25200)
                     /gene="flhD"
                     /locus_tag="ACHKUI_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6599030.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            25980..26408
                     /gene="uspC"
                     /locus_tag="ACHKUI_002867"
     CDS             25980..26408
                     /gene="uspC"
                     /locus_tag="ACHKUI_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6599031.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
     gene            complement(26415..27839)
                     /gene="otsA"
                     /locus_tag="ACHKUI_002868"
     CDS             complement(26415..27839)
                     /gene="otsA"
                     /locus_tag="ACHKUI_002868"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6599032.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(27814..28614)
                     /gene="otsB"
                     /locus_tag="ACHKUI_002869"
     CDS             complement(27814..28614)
                     /gene="otsB"
                     /locus_tag="ACHKUI_002869"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6599033.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVYLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(28781..29767)
                     /gene="araH"
                     /locus_tag="ACHKUI_002870"
     CDS             complement(28781..29767)
                     /gene="araH"
                     /locus_tag="ACHKUI_002870"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6599034.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(29782..31296)
                     /gene="araG"
                     /locus_tag="ACHKUI_002871"
     CDS             complement(29782..31296)
                     /gene="araG"
                     /locus_tag="ACHKUI_002871"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6599035.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQKMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(31366..32355)
                     /gene="araF"
                     /locus_tag="ACHKUI_002872"
     CDS             complement(31366..32355)
                     /gene="araF"
                     /locus_tag="ACHKUI_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6599036.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         8063 a         8765 c         7818 g         8240 t
ORIGIN      
        1 aatctgtaca gtaacttttc agactctgaa aatttttata aaatcgtagt agcccctctt
       61 caaattcccc ttctaaaaat cttcgaaaat atctgacaga tgtctgtagc tgctaagtac
      121 ccgtcacacc agctatgtga ccccctttgc agaattttaa aatatctatg aggtttattc
      181 gataaaccga atcaactatt gactttcttc atagcatctg ttagctccac ctcaaaaaaa
      241 ccttcatcac atttcacagc atctatcaag aagatttaac ctatcaatag ctgtaacatt
      301 ttatagtcct gtgaagtcct ccccagtttt ctctttaaca gacacaaact tctgtgacct
      361 tatgtatcaa tgacttacaa gacatgctct caaggtgctg tatagcctct tatcttcaaa
      421 tacataaaac ccttataaat cagtactgat acatatacag tacgttttta acataatagc
      481 tgttttacgc actaatcgtg ttctacatga aatctgtata gatattaagg tgtctgtaag
      541 tgatttttta agaagggact acgaagattt gacaaggtaa ctatgaggtt gattcctaga
      601 acaaccgaat caactgtaac catcactttc taactttcac gtagctgatt gttatctctt
      661 gcaactgtaa cgtatggtga ccaactgcat gaatagcgtt atatactcct gtatatagca
      721 cttttctagg agtcgagaga gggacgacta acacgataca gtttaccctg aaaagagact
      781 tcacagattc tctatatatt tcaataagtt agggtaaaaa gagactatac tgatactgca
      841 tagaatctgt gtagaattgc atagatagcg cataaataca ccgttaaatt gcataggtac
      901 taacctgttt atgtcctgtt attaacaggt atgttctagg aatcaacctc atagtgtatt
      961 tagaccttga tgattttaga cagccgcccg gttcatgcag cacgtccaca cagtgaagct
     1021 attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact gacggcgaaa
     1081 aatccactga tccgttttat cggtagtgat gatatgacgc aaaaccggga gttatttcag
     1141 gtctggttac aaaaattagc acagtggcat cagaccacta cgccttatct ttttttacat
     1201 acgccagata ttgcccaggc cccggaactg gtacataccc tgtgggaaga cttacgtaaa
     1261 acgcttccag agatcggagc agttccggct attccacagc aatcttctct tttctgaatt
     1321 tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta tggtaagcgc
     1381 gctgtatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg tcgttcgcct
     1441 gcgaatgcag taccgcgttg cctatggcga cggcggtttt agcgaactgc aaagcgctat
     1501 tcgcattcat ggtaacgcgg tggaatatat tcctatcgcg attgtgttga tgctgtttat
     1561 ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt tgcttgctgg
     1621 tcgtctgatg cattattacg gttttcatca ccgtctgttc cgctggcgac gttctggcat
     1681 gagcgcgacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt atatgccctg
     1741 ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc ccggattttt
     1801 acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg ggcgactgga
     1861 cctttgatga acgggtagct gaagtcttcc cggatatgat ccagcgttcc gttcccggct
     1921 attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa cctggtacgc
     1981 aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc
     2041 atcatgataa ttgcaaaatt attgccatcg ataactcccc ggcgatgatt gaacgctgcc
     2101 gtcgtcatat tgacgcctat aaagctccta cgccagtaga cgttattgaa ggtgatattc
     2161 gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg caattcctgg
     2221 aaccttccga gcgccaggcg ttactggata aaatttatca agggctgaac ccgggcggtg
     2281 cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa ttgctgttca
     2341 acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc agccagaaac
     2401 gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa gcacgcctgc
     2461 ataaagccgg ttttgagcat agcgagctgt ggttccagtg ctttaacttt ggttcactgg
     2521 tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt ctctgattgc
     2581 caaaaatcat ctttcacact ggctcgaaac gctgcccgcg cagattgcta actggcagcg
     2641 cgagcagcag cacgggctgt ttaagcagtg gtccaacgcg gtggaatttc tgcctgaaat
     2701 taaaccgtat cgtcttgatt tactgcatag cgtgacggca gaaagcgaag aaccactgag
     2761 cgccgggcaa attaaacgca ttgaaacgct gatgcgcaac ctgatgccgt ggcgcaaagg
     2821 gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact ggaaatggga
     2881 tcgcgttctg ccccatcttt ctgatttaac cgggcgcacc attcttgatg ttggctgcgg
     2941 cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga
     3001 ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt aaactactgg gcaacgatca
     3061 gcgcgcacat ttgttaccgt taggtattga acaacttccg gcactgaaag cctttgatac
     3121 cgtcttttcg atgggcgtgc tttatcatcg tcgttcaccg ctggagcatc tctggcagtt
     3181 aaaagaccaa ctggtgaatg aaggcgaact ggtgctggaa acgctggtta ttgatggcga
     3241 tgagaacacg gtgctggtgc cgggcgatcg ttacgcacaa atgcgtaatg tctatttcat
     3301 tccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgttg atattcgcat
     3361 tgcagatgtg agcgttacca ccacagaaga gcagcgacgc accgaatgga tggtcaccga
     3421 gtctctggcc gattttctcg acccgcatga tccgggtaag acggtggaag gttatcctgc
     3481 gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg tctggtaata ctgccggatg
     3541 cggcgtgaac gccttatccg gcctacaaag tcttgctaat tcaatatatt gcaggggcta
     3601 tgtaggcctg ataagcatag cgcatcaggc agttttgcgt ttgcataacc tcagcgcccg
     3661 tttccgggcg cttttcacgt cttatgcctg aacagctggc tgatcaaacg ccgttaactt
     3721 cagcgcattg cccgtatatt tttcgatata caccagcgca gagttaccgg cacaggcatt
     3781 cgccagctgc gagctgggga tatccgccgt taacacgttc gcactaccgt ttttacacaa
     3841 gccattttcc agatctggcc atgcaccttc atgcacgcat accacgcctt ttttgatccc
     3901 gtcagtcacc accgcgcctg tcaggatctg accgcgcttg ttccagacac gcaccagatc
     3961 gccattcgca ataccaaagc gagcagcatc ttcggtgtga atagtgattg gttcacgatc
     4021 ggcaaccgca tattttttac gcagttccgc atagttaagc tgactgtgta aacggtgtgc
     4081 cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc actcatcagg
     4141 cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca gcgttctgga
     4201 gtaaatctca attttgccgc ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa
     4261 atcaccataa cgaatgtact gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt
     4321 ttgctgccag aaggcattaa acatcggcat agtcacgcgt tgcgcacggg cacctttctg
     4381 agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg tatagatctc
     4441 ttttccgcca ggtttgagta attcagcaag atcggcaaac acgtcaaaat cgttgcgcgc
     4501 ttcaaattgc gcagcgacag cctgcttcat cggcacaata tgctggttgc tgtaatcacc
     4561 ggtcatcgtc aggtcattgc gctcaaacga tgtggtgatc ggtaatacga tatctgcgtg
     4621 tttggctgct gcggtccagt agcattcaga aacgacgatc atctccggtt tctgccatgc
     4681 tttaatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg cccaccagat
     4741 cattttgata ttcggatacg tttgttcttt accgttatgc tgatatttgc tgccaggatt
     4801 ttccagcgca tcgacgatac gggcaacagg aatcgccgtc attccgccat catcagcggc
     4861 ctcgctggcc tgtccggcaa tcgccgcaga catttctggt agcacgccgc caacacgcgt
     4921 cgggttgcca ccgttggagt aatgataaga gaaaccaaac ccgccgcccg gtgtaccaat
     4981 ctgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac catattgctg
     5041 gcgctgaatt ccccagcctg ccatcagcat tgtgcgattt gccgccatca gttcagcgag
     5101 tttgactatc tgcgcttccg gtacgccagt aatttccgcc gcccaggccg cgctcttcgg
     5161 cgtattatcg ctcttacctg tcagatactc ttcaaactgc ggatacccgg tagtgtattt
     5221 ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa tcctaacatc
     5281 agtgccacat cggtgcccat attcggtgcg atccaggtgg cattatcgcc aaagaattcg
     5341 atggtttcgg agcagatagg atcaatggca atcactggtt tgccagattt tttcagctga
     5401 tggaagtatt ccagtccttg ctcatcggta ctgctccagg caattttgag cgtgttcagc
     5461 gggttcatcc cccacagcac caccacctgg ctgttttcca gaatcagcgg ccaggaggtc
     5521 tgctgttcat acacctctac agaaccgacc acatgcggca tgatcacctg tgccgctccg
     5581 gttgaataat cgccgctatg cccggaataa ccgcccgcca ggttcatata acgttgcagt
     5641 aaggtttgcg ctttatgcaa cacgccagaa gaacgccagc cataagatcc ggcaaaaatg
     5701 gccgatggtc cgttagcttt gcgaatgcga tcgtgttgtt catgaatcag ttttaatgct
     5761 tgttcccagc tcacctgtac ataggtatct tcgccacgac ctttcaccgg ttgcagtgga
     5821 ttatcgagat agctttttct caccatcgga tgctgaatac gcgccgtggt gtgtacctga
     5881 tccgccgccg tagactgtaa tgagttcggt atggttttcg ccagcgcgcc tgtcgaagaa
     5941 acaatcttgc cgtccttcac ttctacgttc atcgctcccc aacggcccgc ggtgagaatt
     6001 ttgccgccct tttcttctgc ccatgcgggt agcggtgcag ccgatgtcac caccagcgtc
     6061 cctgccgcaa taccgctatg tttaataaat tcacgtcttg ttaatgtcat aacttcctcc
     6121 ctgatcaacg aggatcactg tttctcggta atatctttgg cgttgtactg gaagtaccgc
     6181 gtcaaaatgt ccagttcgtt ttcgctcatg ctggttcgcg cccccattcc tttggcaatg
     6241 gacggccatg tattgacggt gtaatggtcg gcggcaatag gggcatggca gccagcgcaa
     6301 taggtaccgt caagtttttc agcgtattgc catagcggtt tacggtccgc taatgcggga
     6361 tcgataagcg ccccctgcaa agacgcctga cgccattgat tgccgtattc gtcagcctgc
     6421 cattccccgt ttaccgttag cgccttgata ccttcttcac ttaatgtggc gagcgccagc
     6481 cgttggcctg ccgccaggta gagcgtgttt tcactgccct gcatttgata accctgcaac
     6541 cgaacaattg gctgtttgcc actggcatca acgacggtaa gatcggtacc aggagtcacg
     6601 gtagccagtt cgcctatgcg agaagttttg aaaggataaa tatgtgcgcc attagtaact
     6661 gaagtagcgg cctgactttc cagctcatgc gccgcgttgt catccatttt tatttctggc
     6721 ggaaaatggg caatgccttt atggcaatcg atacaggttt cgccgtcctt ttgcgcttta
     6781 ttatgcattt tctgcgcaga ttcactttgc gaggcaatat ccatggcatc aaaagaatgg
     6841 caactacggc aggttgcaga gtcagttgct tttaattctt tccatactgt ttcggccatt
     6901 tcctggcgat gagcttcgaa cttatcatca ctgtctattt tgccgctaac aaattcatga
     6961 taaatatctt tagatgcttt taatttagca aataaataat ccatccctga ctttggaata
     7021 tggcaatcgg cacattccgc acgtatccct ttctggtttg aaaagtggac agttccctga
     7081 tattcctcaa aaggtttact catcgagtgg caggaaaggc aaaatgcggt atccgacgtt
     7141 ttatgtaagg ctttttgcgc cagcaatacc ccgccgccac caaccacaac cgctatcagc
     7201 aaaaacaata acccaatgcg ttttttccct cgcatagatt cctctaatta aattccagga
     7261 caccgatacc attgtataaa taaatacaca acgaattgac tacttgctta attcggcaca
     7321 acactgcctg aaataatcga taaagaatat gatttattac aatgtaatca ttaattgcta
     7381 aggaataacc cagttgttat ttagaatata tatttgtcag ttttggcaat ttaaatcaca
     7441 taatgaatgt gaatatataa aatattttta gcacaacgct atgcgcactg tcacccgctc
     7501 aacagatcac ggcaatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt
     7561 gaaattgcag gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa
     7621 aatcatttgg cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct
     7681 acgacataac gcgaatactc gtccgcgtgt gcatctgatg acatggacaa tccttgatta
     7741 cgataacgca tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg
     7801 gcatcgagga agtggtgcag gttttccgca cggactcctg ctccggccat aatgattgga
     7861 gcatcacgat gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt
     7921 tgccctgatg tcagtacccg ggcaatgccc aattccgtaa gattattgag tgtatttaaa
     7981 gggttagcgc acatatcgaa ggcacgatga aaagtcactg ccagcggacc ggcagcagcc
     8041 attatttttt ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc
     8101 agtccaggaa aacctaattc gcggactgtg cgcacatctt caagaatggc ggcaaactca
     8161 ccgtcgctgt agcaaaaatc accaccgcgt gggcggataa tcggatgcac tgggatcgtc
     8221 acccgctggc gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttgga
     8281 gctgcgcata attcaactct gtcagcgccg ttttgctgcg ccgttagcgc gcattccatg
     8341 ctgtaacagc aaatttccag taatgccatt tttgctcctt agttacgccg actgctcgct
     8401 ggcaacgatc tcttcaatgg accacggatg aaacttaatg gttgttttac catcggtgac
     8461 cgccagcgtc ggattcggta atcgttcatg ctcacctttt ggcgaactgg ttttaactga
     8521 gatccccggc aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt
     8581 ttctggatca cccggtagaa cgatttcaat atgttcccaa ccttcgtgcg ggtaacgttt
     8641 ttccccgggc cacggtagct ccacaatgga aaactgccag tgcgcaacct gtagcggttc
     8701 atgcaattta aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc
     8761 gcactgttca aacccgcgac gccagcgttc agcggtgacg ttttgatggc aacgcaagga
     8821 aatgtgatcg gcagtcagcg gagtgatatc caaccccaga cggcgggaaa gttcatctaa
     8881 tgcgtgggta aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca
     8941 gttcgccata atcactcttc gtctttcatt aaaagcgcta atttaccctg ttgccctgcg
     9001 ccaaccaacc gctgatttca cgccgcttct gatgcaatgg tgaaaacggc aatacgccac
     9061 gcgcacgttg ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact
     9121 ggtgcgggca atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc
     9181 ttctctcaga aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac
     9241 cgcaggttcg tcagtcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg
     9301 cagttgctaa aaaactgggt atggcaccgc gacaattagc agagcaggtg ctgactcatc
     9361 tggatcttaa cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt
     9421 tccttgatcc ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg
     9481 gtgttgctac gccagaaaaa caaaccattg tggttgacta ctctgcgcca aacgtggcga
     9541 aagagatgca tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gttcgtactc
     9601 tggagttcct cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt
     9661 tcggtatgct gattgcgtgg ctggaaaagc agcagcagga aaacgccggt gaaatggagc
     9721 tggctgacct tgaaggtttc taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt
     9781 ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg
     9841 agatgtggcg caaactggtc gacatcacca tgacgcagaa ccagatcacc tacgatcgtc
     9901 tgaacgtgac gctgacccgt gatgacgtga tgggtgaaag cctctacaac ccaatgctgc
     9961 cgggtatcgt ggcggacctg aaagccaaag gtctggcagt agaaagtgaa ggcgcgaccg
    10021 ttgtattcct tgatgagttc aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga
    10081 agaaagatgg tggctatctc tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg
    10141 aaacactgca tgccgatcgc gtgctgtatt acatcgactc ccgtcagcat caacacctga
    10201 tgcaggcgtg ggctatcgtc cgcaaagcag gctatgtacc ggaatccgta ccgctggaac
    10261 accacatgtt cggcatgatg ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg
    10321 gtacagtgaa actggcagac ctgctggatg aagccctgga gcgtgcacgc cgtctggtgg
    10381 cagaaaagaa cccggatatg ccagccgacg agctggaaaa actggctaac gcggttggta
    10441 ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac cacggactac atcttcgact
    10501 gggacaacat gctggcgttt gagggtaata ccgcgccata catgcagtat gcttacacgc
    10561 gtgtattatc cgtgttccgt aaagcagaaa ttgacgaaga gcaactggct gcagctccgg
    10621 taatcatccg tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa
    10681 ccctcactgt ggttgcccgt gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc
    10741 tggccggtct gttctctggc ttctacgagc actgcccgat cctcagcgca gaaaacgaag
    10801 aagtgcgtaa cagccgtctg aaactggcac aactgacggc gaagacgctg aagctgggtc
    10861 tggatacgct gggtattgag acagtagagc gtatgtaatc gcgttatacg gcaacagccg
    10921 atgtcattgc agacatcggc ttttttatca ttaacgataa tttacaatca cttcattccg
    10981 ttgaatcttt agcggcggaa ttaaccgccc accgccaggc acttcccaga taaagcgcaa
    11041 cggttctgcc gccggtatac cggaaaaggc cacggtggtt ccactctgcc cctctaattc
    11101 gacacaacga gattgtgaac acaagcgaac ccgcagtcct gcaggtgtcg gaccgataag
    11161 ctgataacgc cacgccacca gcgtcatcaa tcctgcagca ggttgtcgag tagaaagagg
    11221 cgctgacgac atcgactcac cacggtgatt cagcgtaacg cccacactac tcgcctgcca
    11281 catcccctcc ccggcggctt gcaccagcag cggaaacaat aatattgcta ataaagttct
    11341 catcatttac caccaattgt cgccgtcatg cggatatgtc ggttatcaga tagttccaga
    11401 ttcgacagca ccactaactg cggcaagctg cggcgcagga agcgagacaa taatggtcgc
    11461 agcgcgtggt tcaccaacag taccggcggc gcacccaaca tctcctgacg ggatagcgct
    11521 tcctgagttt gcgccaacaa gcgatccgcc agccctggct ccagtccccc cccgccctgc
    11581 aatgcctgta gcaacaaacg ttccagcggt gtatcgaggc caataacatg gacttcatct
    11641 ttgccaggaa accactgctg ggtaatcgca cgtcccaacg ccacgcgcac gacagcggtt
    11701 aattcatgcg gatcgctctg gatgggcgca tgttccgcca gcgtttcgag aatggtgcgc
    11761 atatcgcgaa tcggcacttt ttcatcgagc agattttgca gcactttatg cagtgtggta
    11821 agcgtgacga cgccaggaac gagatcttcc gtcagctttg gcatctcctg ggcgacgcga
    11881 tccagcagct gttgcgcctc ctgacgacca aacagctctg cggcatgctg gctaatgagg
    11941 tggttaagat gcgttgccac caccgtgctg gcctcaacca cggtgtaccc ctgaatctgc
    12001 gcctgttctt taagcgcact ttcaatccag atagcattca ggccaaatgc cggatcaacg
    12061 gtcgcttcac caggtaatgt tccggcggca gtgcctggat taattgccag ccagcgcccc
    12121 ggataagcat caccactccc catttcgaca cctttcatca agatacgata gcgggctggt
    12181 tgcaaatcca tattgtcgcg aatgtgtacc actggcggca gaaaccccat ctcctgggca
    12241 aatttcttgc ggatactgcg tatacggccc aacaactcac catcctgctg gaaatcgacc
    12301 atcgggatca gtcgataacc cacttccatt cccagagaat cttccagttg tacatcgttc
    12361 cacgtcgctt cgacaacggt attattctct gccattttta ccggttttgg ttcagcaggc
    12421 gctttttgtt cacgtccgcg gatccaccag gccagcccca gcaatccggc agtgaacagc
    12481 aaaaatacca ggttcggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt
    12541 aataacataa cgctcggatt actgaaaagc tgattcacca tctgctcgcc aacatcctga
    12601 tcggtgctga cacgtgtaac gatgacgccc gcggcggtag aaatcaccag tgccgggatt
    12661 tgtgccacca gaccgtcgcc aatggtcaat agcgtataac tttccgccgc gtgccccatg
    12721 ctcatgccat gttgcagcac accgaccagc aatccgccga caacgttaat gaccatgatg
    12781 aggatcccgg cgatggcatc gccgcgaaca aacttacttg ccccgtccat cgagccgtaa
    12841 aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cttcatcttc accaatcaat
    12901 ccggcgttaa ggtcggcgtc aatcgccatc tgcttacccg gcataccatc gagaacaaag
    12961 cgcgcgccca cttccgcaat acgcccggca cctttggtga taaccataaa gttgatgatc
    13021 acgagaatga cgaacaccac gataccgata gcgaaattgc caccaacgag gaagtgaccg
    13081 aacgcttcga ccacctttcc agccgccgcc gcgccggtat gcccttccat caaaatgata
    13141 cgggttgaag ccacgttaag tgccagacgc aacagcgtgg taaacaacag gatggtcgga
    13201 aacgcagcaa actcgagcgt gcgctgggta aacatcgcca ccagcaacac catgatcgac
    13261 aaggcaatat tgaaggtaaa cagcaggtcg agtatgaatg cgggcagtgg cagtaccatc
    13321 atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccattg tgtcgatttc
    13381 aggtttgcag gcaggcgcag catcgcggcc agattactca tgggtcggtt tctcgttaat
    13441 aaaatccaag gcttccggca ccggaagatg agtaggttgt acagggcgct gtccaccagc
    13501 cagacgccag cgtttcagtt gccagaccca ggccagcact tccgccaccg cggcgtacag
    13561 ttgacccggg atttgttgac cgatctccgc atgtcgatac agcgcacgcg ccagcggcgg
    13621 cgcttcaagc gtcgggacgt tattttcagc gccaatttca cgaatgcgca gcgcgaccag
    13681 ccctgcacct ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc
    13741 taccgaatag tgggtcgggt tattgacaat gacatccgct ttcggcacat cggccatcat
    13801 ccgacgccgt gcggccgctc gctgcatctg acggatccgc cctttaacat gggggtcgcc
    13861 ttcgctttgt ttgaactcat cacgaatatc ctgccgtgac attcgcagct ttttcaggtg
    13921 gctgaagatt tggaaaaaga cgtcaaatcc caccattgga atgacaccaa gcaccaccag
    13981 cagcgcgcat agccctacca gatccatcgc attacccatg gcggtaatcg gagactcggc
    14041 cattaagcgc atcatctgcg gccagtgatg ccagagaaaa aaccccgtca cgctgccaac
    14101 caggatggtt ttcaaaattg ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg
    14161 tttaatgccc ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt ttccgctaaa
    14221 taccagcccc cccagcataa ccggagaaat aatcgccacc agcaccacgc cgctaatcag
    14281 cggtagcagc gccagcatgg cttctctgat cagcagaata atctgcccga ggatcagatt
    14341 cgggtcattg ataatactgt gatcaaaatg cagccctgcg gagagcatgc ccgacaatcg
    14401 acgggccagc gacacaccgc caaaccagat aacactaacg cccaccagca aaatcagcag
    14461 tgaagtcagt tcgcgggaac gcgggatttg cccctcttcc cgcgcttttt ctagtcggtg
    14521 aggtgtgggg gcttctgttt tgtcgtcgct ctcgtcagac acgtcgccaa tcctggaaag
    14581 agtattaagc gtaaatgatg ccagagcgca aagtgttcaa tggtttgagt aaggggcaaa
    14641 acaggcggga tttagggctt ttgccacagc acatcaggca tggtgacatg tttgtcgggt
    14701 acggcgtaaa cgccttatcc aaccttgaag atctgatgtg acgcggccac gtcacatcag
    14761 gcaatacaaa tcaaaatcca agactatcca acaaatcgtc cacctgatcc tgactggcta
    14821 ccacaccggc tttgctggta tcgacctgag gtccattaag caaactctgg ttttcacgtt
    14881 ttggacgcga ctcctgttcc gggatgtttt ccaacagcac catcagcaac tgacgttcga
    14941 tctcctgaat gacatccatc atccgcttaa tgacctgtcc ggtgagatcc tgaaaatcct
    15001 gcgccatcat gatttccagc agttgcgcgt tagtaaagct ggtatgtgcg gggacatctg
    15061 ccagaaattg ccgcgtgtct gttaccagtt cacgggcgtc ggcaaggtca atcggatcgg
    15121 caaaccagtc atcccaacgt tgggttaacg cttttgctga tttctccatt tgatcctgat
    15181 gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc agcctgggcg gtcatctgca
    15241 caacatagta caaacgatcg cgcgcatcgg ggatagcttc cgcggcttcg gcaatggcct
    15301 gatccagccc cagttcccgc aaactgtcgc gcagcatacg cgtcaggctg ccgatgcgcg
    15361 caatgatatc gccagctgaa tgctcgtcag caggtttgat tgatggttgc atcatagtcg
    15421 catcctcaca tgcccagttt ctcaaagatt ttgttgagtt tttcctccag cgtcgcggcg
    15481 gtaaatggtt tcaccacata gccactggcc cccgcttgcg ccgcagcaat gatgttctct
    15541 ttcttcgctt ctgcagtcac cattaacact ggcaatgccg acatcgcgcc atccgcacga
    15601 atcgttttca gcaactccag gccatccata ttgggcatgt tccagtcgga gataacaaat
    15661 ccataaccgc ctgcctgcaa cttattgaga gcgtcgaggc catcttccgc ttcctcaaca
    15721 ttattgaatc ccagctcttt cagcaggtta cgcactatgc gtcgcatggt ggaaaagtca
    15781 tccacaacca aaaatttaag ttctttatcc gccatttcac actcctgatt taaatacgta
    15841 tcgcctgtcc ggcactaatt tttgccagca tttgctgact tacctggcta agatcgacca
    15901 cttcgcagac accacccata ttgatggcct cgcgcggcat gccgaacacc acgcaacttg
    15961 cttcgttttg cgcaagggtc catgcccccg cctgacgcat cgccaacatt cccgccgcgc
    16021 cgtcgttgcc cataccggtt aggattaccc caaccgcatt acgccccgcc tgtttggcca
    16081 cagaatggaa caacacatct accgatggcc gatggcgatt aaccgccggg ccatcgtgaa
    16141 ttttgatttg ataatttgcg ccactacgcg ccagctccat atgccgatcg cccggcgcga
    16201 tataggcatg tcccggcaag acgcgctctc cgtcttcggc ttctttaact ccgatctggc
    16261 aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc gggcggcata tgctgggtaa
    16321 ttaacagtgc cggactggaa agcggcaacg gttgcagtac gtgacgaatt gcctcagttc
    16381 cacccgttga agcaccaatc gcaatcagtt tttcagaact caacaacggc cccgccttca
    16441 gcgttgtcgg taccgacaat ggcttatgtg ctgcaaggct cgcctttgct gccgtacgca
    16501 ccttttcagc aatcatttcg ctatatgcca gcattccttc gcgaataccc agttgcggtt
    16561 tggtgacaaa atctatcgcc cccagctcca gcgcgcgcag cgtaacttct gaccctttgc
    16621 cggtcaggga agaaaccatc acaacgggca ttggacgcaa acgcattaat ttttcgagga
    16681 aatccagtcc gtccatccgc ggcatttcaa catccagcgt caatacatcg ggattgaatt
    16741 tcttaatcaa atcacgcgca accagcggat caggggcggt cgccaccatt tccatgtcgc
    16801 tatggctgtt gatgatttct gtcatgatct ggcgcatcag tgccgaatca tcgacagata
    16861 acaccctgat tttgctcatc gttaatcctt acttagcgca tacaccgtct gaccacgcag
    16921 catgaagcgg cgctcaaggt ggctaaagtt ttcagagtga cccgcaaaca gcaatccgtc
    16981 gggtttaagg agcggaacaa agcggcgcaa aatcttctgc tgggtagttt gatcgaagta
    17041 gatcatgacg ttacgacaga agatcgcatc aaacggcccc ggcacggtgt actgtttcgc
    17101 cagtaaattc agcggggcaa aatcaacata gttcgccagc tcctgacgca cgcgtaccag
    17161 cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc agttgctgcg gcgtcaggtt
    17221 tttcaactct tcatggcgat aaataccgct tctggctttt tccagcactt cggtgtcgat
    17281 atcgctggca aacactttcc agcgtccggg cgcggtgccc aatgtatcag ccagcgtcat
    17341 cgctatgctg tacggctctt cgccggttga agccgccgca ctccatacgc gatactcgcc
    17401 agaacggcga cgtgcgtgat ccgcgagcag aggaaaatgg tgtgcctcac ggaaaaatgc
    17461 cgtcagattc gtggtcagcg aattgataaa cgcctgccac tcaccgctgt gctgattaga
    17521 ttccagcaag ttcaggtaat gaccgaaatc cgccagtccc agcgcacgca aacgacgaac
    17581 cagtcggttg taaaccatgt cgcgtttatg gtcagccaga acgatcccgg ctcgttgata
    17641 gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc gccaggcgct cggtcatctg
    17701 taacaataaa gacgtttgcc cacagggcag agatgaagtc atagcgcctt ctcaatcact
    17761 tcaggatacc actggcgcaa tttgtaactg cgccgactca tgtcgtgcta cttcatgttc
    17821 ttcaagggta aataccgcca cacgcgacga aagatggtcg gcctgattca gtcgttgcaa
    17881 atcgtcgcga aatgcccaaa agacatgccg ttactgccaa tctgcaagat cccgcagaga
    17941 atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa cgctccccat
    18001 caggcggcaa tgaccgcttt agtaaatact cgtcaaaatg tttcccagtt tggatcttgt
    18061 tcagcaattc gcagtcgtgg ctgtgccggt ggttgctcac tggcaggacg agatggtgtt
    18121 tgcggtttat tggtgagtgg gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa
    18181 cgactcgcct gttcttccag cgcagctgcg gcggcagctg attcctgcac cagcgatgcg
    18241 ttctgttgcg tgacgcgatc catttccgaa accgccaatg cgacttgatc gatgccacga
    18301 ctctgttcat ccgatgctga tgcaatctcg cccataatgt cagtcacgcg agtgacagca
    18361 ttgacgatat tgttcattgt ttcccctgcg ctttcgacca gcaccgaacc ggtatcaacg
    18421 cgcgagacgg agtcttcaat gagggctttg atctcttttg ccgcctgggc gctgcgactg
    18481 gcaagattac gcacttcgcc cgccaccacg gcaaaaccac ggccttgttc acccgctcgc
    18541 gcggcttcaa cagcggcatt cagcgcgagg atattagtct ggaaggcaat accatcgata
    18601 acgctgataa tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat cgttttcact
    18661 acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc
    18721 gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg
    18781 gcggcagttt cttccagcgc ggatgcctgc tgttcagtac gggaggaaag atcggtgttg
    18841 cccgccgcaa tttcacgggt accggcatag atggcatctg aaccttcgcg cacatgagtg
    18901 acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc gcccatttca
    18961 ctgcgcccgt caatggtcag ggtattcgcc aggttaccac cggcaatttc gcgaatatgc
    19021 gcaataattt ttgccagcgg tgtaagcaac atacggcgaa tgccgtacca cgccaccagc
    19081 agaatcaata ccaccaccag cgcgataacc gccagttgcc actgggcaaa tcggtaatca
    19141 tctgcgttgt cagtgacgat atcgcgatac agtttttcac tgctgagggc gtactgagca
    19201 aacgcttcgc ccattgcatt ttgcattccc tgggttggct gagcgaaata agctccagta
    19261 ttgccataat caagataatc aatcagttca gttaacgctg tgtgatagtt tttatatttt
    19321 tcatcaatat tacgactggt agcgaccatt tcaggtaacg gtgccatgct tttgaatttt
    19381 ttataatgag tcgcggcctg ggccaatgtt ttcctggcac tatcgagcaa ctcaactttg
    19441 gcattacttt gctgattgga tgaatccatc atcatccgca ccgctgaacg actcaggtta
    19501 atgcgcgttt gtagcattaa atcccatgtt gacgtcagct cgccctgctg ttcccgtaat
    19561 tgattggaaa ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa cagactgccg
    19621 gaaataagct gtaacagtgc gaataccccc agcaccatta ccaacagcgt gactacgcgg
    19681 atacggttaa tcataaggca ccttcctgaa aacaagttga tctcgttatc ggcaaggagg
    19741 ggggaaactt tattgctgat gccacccgcc gcgaaattga aataaaaaac ccgatgcgca
    19801 gatcatcggg ttcatttcaa ttgaggacat cgggagaatt acgccacttc tgacgccgcg
    19861 ctatctaaca gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac
    19921 atgcggtcgc ccagtgcgcc cagtcctgtg agatattcgg ttgaaagcgt cacggcaaat
    19981 tccggtgccg gacgaatttg ctccgccgtc aatgaaagca cgtctgagac gccgtcaacc
    20041 acgatgccga ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag
    20101 tccacatcca cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc
    20161 agattcgtga cgcctttgat aaacgctggc gtgttcgcaa tccgtgtcac ctgatcgtag
    20221 ccacggatct cctgcacttt cagaatatca ataccgtact cttcatcacc aagggtaaat
    20281 accagaaatt cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc
    20341 atattgttac ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta
    20401 tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg
    20461 cagcagaaat gccggggact ttgcgatagt tactttcaag gtttttaacc acaacctggt
    20521 gttgaccaat taattgatcc actagcaagg cgtagcggcg accgccactt tgtaagatca
    20581 ccacaattcc ctgggtggct tcggttttcg cgcccgcgac gttgaacact ttccacagtt
    20641 cgacgatagg cagatattca ccccgcactt ccagcacccg ctcgtcgcca gccagtggat
    20701 gcagatcggc ttcacggggt tgcagtgatt ccatgacagc attcagcggc agaatgaaaa
    20761 cttcatccgc aacgcgtacg gacatgccgt caagaattgc cagcgtcagc ggcagtaaaa
    20821 tgcggatcgt agtgccagta ccctgcatcg actggatctc gacatgaccg cccatctcct
    20881 ggatattacg tttaacgacg tccatgccga cgccacgccc ggagacgtcg gtgacctgct
    20941 ccgccgtgga gaagccaggt gcaaatatca gcattgcaac ttcgtcgtcg ctcatgtttt
    21001 cgctgacggt cagaccttgc gaggccgctt ttgccagaat tcgttcacgg tttagccccg
    21061 ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta
    21121 aatttccgac gctgttttta cctgcagcga gccgtttttc tggcagttca ataccgtgat
    21181 cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat gcgttcaatc aggctcttgt
    21241 cgagttcagt agaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca
    21301 gatcacgcac cagccgggga tagcgactaa agacatattc catcggcatc atgcgaatcg
    21361 acatcactga ttcctgcaaa tcacgagcgt tacgttgtaa ctgccccatg ctggtaatca
    21421 aatcaccatg attaaccggg tccagttcac tggagcgctg agcaagcatg gactgggtga
    21481 tgaccagctc gccgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc
    21541 tggtggattc actgctgcgc gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt
    21601 cggctgccag tttaagcact ggtggggtgg atatttttgg cgagacgtct actgtttcaa
    21661 aggtaatctg accggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga
    21721 tgtcgcccgg taatattgcc gagagagaat ccgccccttt caccacgtca gttaacgttg
    21781 tcagatgccc caactcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga
    21841 taattcgtcg cggtgactga ctacgactct gctcatcttg cggttcactt ttggcaacca
    21901 cacttaatcg ggtcactgca gatggcgttt cgcctttcgc ttctaatgcc agttgacgca
    21961 aggcctggca gatataatcg aagctggcgg catccggctc ttgcgactgt ttataagcgt
    22021 cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga
    22081 gttgcatctc acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct
    22141 gcaaaacgct gaagccaaag gttcctgccc ctcctttgat cgagtgggca gcccgaaaga
    22201 tggcattcaa ctgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca
    22261 tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca
    22321 tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca
    22381 acctcaggtt tttccagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa
    22441 atggcctgtt cggcgtgttt gttcagtacc agcaggctga tgcgacggtt gatggcatca
    22501 tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc cgacgacacg taacactttg
    22561 ccgccattca acccgccaac catcagttcg cggcgggatg cattggcccg atcggcagaa
    22621 agctcccagt tgctatatcc tttctcacca ctggcgtaag ggaaatcatc ggtatgaccc
    22681 gaaaggctaa tacggttggg aataccattc agaacgggcg cgatggcgcg cagaatgtcg
    22741 cgcatatagg gttcgacgtc ggcactgccg gttctgaaca tcgggcgatt ctggctatcg
    22801 atgatctgaa tacgtagacc ttcctggacc agatcgattt tgagatgtgg acgtaacgcc
    22861 cgcagtttcg gatcggactc tatcaattgg tcgagatcgc cacgcaattt ccgcaatcga
    22921 ctttgctcca tgcgtttttt cagctcttcg atgttcggct gcttattcac ttccccctgg
    22981 ctttgggtgt aatcatcacc accgccggga attgggcttt cactattaga aatgcgatcg
    23041 ccgcccgtaa ccgctgtcgc cagcggagtc cggaagtact ccgcaatctg aatcagctct
    23101 tttggactgg agatagagat cagccacatc accagaaaaa aggccatcat tgcggtcata
    23161 aagtcggcat aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt
    23221 cgtttgacga caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt
    23281 gttgcggatt tttcaccgca cgcacatgct cttccagctc aataaacgac ggacgttcgc
    23341 tggaatagag cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat
    23401 tagaaagcag agtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac
    23461 gtaaaacact cgctaatggg gaaataaatc cgtaagccaa taaaataccg aggaaagtcc
    23521 ccaccatcgc atgagcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta
    23581 acgcgtgaac gacccccatt acagccgcaa caataccaaa cgccggaagt gagtccccga
    23641 ccagcgccag actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat
    23701 ccatcagagc ttcgatttcg aaggtgttca tgtgaccact gataatcagg cgcagataat
    23761 cgacgataaa ttcaagcatg acgctatccg caaggatgcg tgggtagctg gcgaagatct
    23821 cactctcacg gggattttca atatcgcgtt ccagcgaaaa catccccatc tgccgcgatt
    23881 tcgccatcaa ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg
    23941 agcgacgaaa cagcaacggc agcgccttca gcgtgccttt aatcgctttg ccattattgc
    24001 cgacgataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga
    24061 gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca
    24121 ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc
    24181 agtcgtcagg cgttaacacg cgataggggc aaaaaaaagc agcggtacat cgttaccgct
    24241 gctggaatgt ttcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca
    24301 gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg
    24361 gcggctgaca taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat
    24421 tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca
    24481 atgtccaggc acgggttaat gccagcagcg gtccttcttc tgcttgcggg cactgttcaa
    24541 ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaagcca gttttcagta
    24601 aaaactgcca tgcattacag aacatcgaag catgaacatt ttgttcccag gtcataaacc
    24661 agtcggttga gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataga
    24721 gttttatcag acgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc
    24781 ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt
    24841 cactcatgat caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag
    24901 caagcgtgtt gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc
    24961 ttgcgtcaac tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagttgatt
    25021 ggtttctgcc agcttaacca tttgcggaag agtcagtgcc gctaacgttg tcgccatttc
    25081 ttcatttatg ccgagacgaa acatagcgga cgctttgtcc tgaacaatca gacgctgtgc
    25141 aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat
    25201 tattcccacc cagaataacc aactttattt ttatgcggtc acaccgcacc ccgtgatgtc
    25261 gccgggaagc cccggtaaaa aataattagc attagaatag ttgcgataag ctgcaataag
    25321 cagacccacc tttttggttt aataagtcct tacaaataga agggggtctt tacacttatc
    25381 taagattttt cctaaatcga cgcaactgta ctcgtcacta cacgcacata caacggaggg
    25441 gggctgcgat tttcaataat gtgtgatgca gatcacataa aacgctcaat tacttaacat
    25501 aaatgtataa gtcatacttt tgttttgggt gtatttcaat ctgttaaaaa ttttttcgct
    25561 acactggcaa gcaaaaatga aacaggaata atcgaaatgg gatgttgcgc acagttaaaa
    25621 taactcaccg taaataatca tctgctataa ataatcgctt taatgcaata cccgataagc
    25681 catttttaaa caaacactta ccgcacaaca aactaattaa ctacaaagaa aggcaacaat
    25741 caacctaatg tcaataaaat gttttgcata tattaaccac ttgttcaata tgggaaatca
    25801 gtacattatt ttcttataaa tacagttgaa atcatatgat aaccaagagt gattgattaa
    25861 ttgtctttac taataatcag actaatattt acctgtttaa ctgagttggg attgcgtcgt
    25921 ttctccatta ggagtaaagc tttaatgtca cctgaagttc acagaataag gaacaggtta
    25981 tgagctatag caatattctt gtcgccgttg cggttacacc ggaaagtcag caactgctgg
    26041 caaaagcggt atctatcgcc aggccagtaa agggacacat cagtttaatt actctcgctt
    26101 ccgacccgga aatgtacaat caattagctg cgccgatgct tgaagattta cgcaatgtga
    26161 tgcaggaaga aacgcaaagc tttcttgata agttaattca ggatgcgggg tatcccgttg
    26221 acaagacgtt tattgcctac ggtgagttaa gcgaacatat tctggaagtt tgtcgcaaac
    26281 atcatttcga tttggtgatt tgcggtaatc acaatcacag tttcttttcg cgagcatcct
    26341 gctcggcgaa aagagtgatt acctcaagtg aggtcgatgt gctgttagtt ccacttacgg
    26401 gagattaacc gcacctacgc aagcttagga aaggtagcaa ctttatcgcg ctgctggctt
    26461 tctgcgcttc gcggaactat ctgctttagg tcgctaatga agcactcctg ccagtggtta
    26521 atatcgtttt tcacgataac gtccagcatt tctgcatgac gggaaatacg ttccgccagc
    26581 gacatagtca atgcacgatc cagcgcagct gcaacttcgt cacgatcgta gggattaaca
    26641 attaacgccg acgttaactc gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc
    26701 gggttagcag ggtcctgagc ggcaacatac tcttttgcca ccaggttcat cccgtcacgc
    26761 agtggcgtca ctaagcccac gtcagagtag cggaatattt tcatcagtaa tttacggtca
    26821 aaatgctgat tcaaataata aagcggcgtc cagcctaatt gcccatattt accattaatt
    26881 cgtccggctt cattttcgag ctgatgacga atatcctgat aggcttgcac atcaccacga
    26941 gacgttggcg cgatctgggt ataacgaatt ttaccgtgat gttgcggata tttttccagt
    27001 aacgcttcat aggcgagaaa acgctccggc aaacctttgg aataatccag tcgttcgaca
    27061 gaaaagatat tttgtacgtt tttcagttct gctttgagtt gcgccagttt tggcggcagt
    27121 ggaccggcag cctgtttcgc tatttctttc ggctcaatgc cgatcgggta aacttccgtt
    27181 cgaaacgctt tgccgcaggc cgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg
    27241 ttagaaaggc aatccaggaa cgccagacga tcgttctccg tctggaaacc cagcaaatca
    27301 taatcacaaa gctgttcaag caaggtgtca tatgtcggca gcgcgttgaa gatttccggt
    27361 gtcgggaaag gaatatgcag aaagaaacca atgcgattat tcacgccccg tttgcgtaat
    27421 tcatgcgcaa agggcaacag gtgataatcg tggatccaga taatgtcatc gtcttgcaac
    27481 agcggcagta atttatctgc cagcaacgca tttacgcgta gatagccatc ccaggcagga
    27541 cgctgaaatt gcaccagatc gagccgataa tgaaaagcgg gccagagaac ggcattggag
    27601 aatttgttgt agtattcgtc aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg
    27661 ttaccctttt tcaccttttt tagcggctga tcctcattcc ctgtttcacc gctccagcca
    27721 aaccacagtc cacctgcggc tttcagtgcc cccagtatgc caacggcaag gccaccggca
    27781 ctggcggcgt gctcgtctgg tggtgcaatc cggttagata ctacgactaa acgactcata
    27841 gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg gtggttatca tttcaagcca
    27901 gctccagaca tccggcacgc ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat
    27961 ttttactgac attccgccca gccggttaac gactgcgaag ccagattcat cggtcagatc
    28021 atcgcccaga aatacgggcg ttcgcccgat aaagggagct tcctgcataa aagcagcaat
    28081 cgcctcacct ttactggtac ctttcggttt gatctcgaca acacactttc cctgctgtaa
    28141 cgccatttgt ggccagatct gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg
    28201 ctgcggagcc tggcgataat gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc
    28261 gggatactgg gcgatgaccg tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg
    28321 cagataaacg atatgcgttt taccattgat gtcacggcgc tccgccccat gcacgcccgc
    28381 taacgggaag cgataaggtt ttgccagtgc gtcaagctcc accattgagc gccctgaaat
    28441 caatgccaat gcaccatcac ttgcggttgc cagtagctga agtccttgca gaatagtgtc
    28501 aggcacgacg acttgatcgg gatgcggttt gatttccgcc agcgttccat cgagatcaaa
    28561 aaaccaggca tatttcgcag atagttcagg ggtttcggtt aacggttctg tcacccggtt
    28621 ctcctccttc tcttttctta ttgtgttgcg cagaacgcgc tttacagaca tgtaagtata
    28681 gacagtgtga cgggggtcgc catttgcaac gaagaacagc caaccgttgt ggcggctggc
    28741 tgtggtggga aaaaacgcta aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt
    28801 tttgcttgta acggtcgaaa atcaccgctg ccagcaggat taagccgcga actacgtact
    28861 gtgcgaaagg agaaatatta agcaggttca tggcgttttc cacggtgcct aaaattaaga
    28921 tacccgccac cacatatgag atttttccga tgccgccttt cagagaaacg ccacctaaaa
    28981 cgcaggcgga gatcacaatc agctcatagc caatcgacgt cattggctgc ccactggtca
    29041 tacgtgaagc cagaataatt ccggctatcg ctgataccag gccagagaga acaaagataa
    29101 taattttggt gcgaacaacc ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa
    29161 tcgccagggt attacgacca aaggtggttt tattcagcag caagccaaag atgatcaaac
    29221 acgcgacggt gagccagatt ggcgcaggca gaccaaacca gttggcgtaa ccaagggcaa
    29281 agaagctttc atcttcgata ccgaccgctt taccgtctga aatgatgtac gccagacctc
    29341 gaacaatctg catcgttgcc agtgtcgtga tgagggcatt tattttcagt ttggcgataa
    29401 caaagccatt gaccaggcca cagagaacgc ccagcaacaa acccgctgcc acgccaatcc
    29461 acaggctttc agtcaggttg ataaccaccg cagtggtgac acccgcacag gcaattacgg
    29521 aggcgacaga aaggtcaaag tcaccggaag caaggcagaa caacatgcca cacgccacca
    29581 tccccgacat agaaattgcc aggcccaacc ctttcatatt aatgaaggtg gcaaaatttg
    29641 ggacaaaaat ggcacaggcg ataaaaagca ccgcaaacac caccagcatg ccgtactgat
    29701 cccagatacg cccaaagctg aatgacgact taggtgctcc agaacccgat gtagaaacag
    29761 aagacatcat actctcctta ctcaggcaac tgcctggctg actttaggca tcgcaaggct
    29821 cagtgcctga cgctcatctg cctgctcgtg taacaattca ccggcgattt caccttcgcg
    29881 catcaccaca atccggtcgg caacgccgag gacttcaggt aagtcgctgg aggcaaacag
    29941 caccgccaca ccctgcgccg ccagcgcata aatcacgtta tatatttcgt gcttagcacc
    30001 aacatcaatg ccgcgcgtag gttcatccag caaaatgacc ttcatctctt ccgataacca
    30061 gcggcccaga atggcttttt gctgatttcc acctgagaga ttcataatca gttgctcagc
    30121 gcccggcgtt ttgatgttga gcgaacgaat gtggtgatcg gcattgtttt cttcccaacc
    30181 gttgttgatt acacaaccgc cgagcacatg tttgcgtctg gcactgatgt tgatattgtc
    30241 gcgaacggag tgcacaggaa taatgccttc cgctttgcga tcttccgggc agagcatcat
    30301 gcctgcggta atagcgtggc tcggtttacg aatatcgatc ggctgttggt cgatataaac
    30361 ctgaccggcg gtgatttgcg taccgccaaa catgcctttc attaattcgc tacgccctgc
    30421 cccgaccaga ccaaacagac cgacaatttc tccactgcga accgccagac ttattggcgt
    30481 acgcacgcct ggtgctttca cagcatcaag acgcagccgc tcctcgccat aactacgcgg
    30541 ttgccagccg tagatatcgc caatgtctcg cccgaccatc gcctgcacca gtgcgtcgtg
    30601 gtcaacctgc tgcatatcgg taaaggtttt gacataacgc ccatctttaa agacggtgat
    30661 ggcatcgctg agggcaaata tttcttccat acggtgagaa acgtataaga tcacccgccc
    30721 ctcttttcgc agttcacgaa taacgcggaa aagattgtcg atttcgcggg cagagaggga
    30781 gctggttggc tcatcaaagg cgataatttt ggcgttacgc gccagcgctt tggcgatttc
    30841 aaccatctgc cactgaccaa tggagagata tttcagcggc gtgtccgggt caatatccat
    30901 accaagatgt ttaatttgta aacctgcctc ataattcagc aatgaacgat tcacaatgcc
    30961 gcctttatgc ggcagctggc cgagatagat gttttccgcg acggtcattt ccggcacgag
    31021 atgcagttcc tggtaaataa tcgccacccc cgcgttaagt gctgcggtcg tgtcggaaaa
    31081 ggacattttc tgcccattaa tcactacaga acccgtggtt ggcgcatagt ttccgctgag
    31141 gatttttaag agagttgatt ttcctgcgcc attttcaccc atcaacgcat gaacctgacc
    31201 ggcatagcag tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg ttttaccgat
    31261 gccgcgaaat gagagatacg gggtagactg ttgcataacg tctccgtgaa tcactggtcg
    31321 ttcacacccc ctctgcatca tgcagagggg aatttttccg gcaaattact taccgcctaa
    31381 accttttttc tccagttctt ctttaaagtt gtcacgcgtg atcagcacca cgtcggtaac
    31441 ttcggtgaat ttggtcggtt caacaccttt tgcaacccag ttgtaaagca tttcgctgga
    31501 cttataacca tgtacgtccg ggcttggcag cagggaaccg tagaagccgg ttgcctgtgc
    31561 cttagacagt tcactcaccg catccacacc gttaataccg ataccgatga tatcggccgc
    31621 tttaaagccc tgaccttccg tcgcgcgtac gccgcccagc acggtgctgt cgttcatacc
    31681 gacaatcagc caatgtttaa cttccggatg ttgaaccagc attgagttgg cagcgtcaaa
    31741 tgcccccggg atgtcgttag atttggtagg tacctgataa atttgttttt ccgggaatcc
    31801 tgcagctttc agcgcatcca tagaccccgt agtacggcga cgagccgtat ccagttcgtt
    31861 ggaggtaatc gccatcaccg cgctttcttt gacatcccag ccacgtttct gcatctcttt
    31921 atacagttcc tggccctgac gttcgccaat tttggtcgca gccatcatca ccagcggaac
    31981 ggtatccatt ggcttacctt tggcgttaac aaactggtca tccacggcaa tgactttcat
    32041 atcgtagcca cgcgctttcg ccacgatggc agagccgagt ttcgggtccg gagtacaaat
    32101 aacgaaacct ttcgcgccac tggcagccag gctgtcgata gcgttcaatg ttttttcgcc
    32161 atccggcacg gcaatcttaa taacttcaaa ccctaaatcc ttcccggctt tatcggcaaa
    32221 cttccattcg gtctggaacc acggctcttc cggttgcttc accaggaaac cgagcttcag
    32281 gttctccgcc atagcggatt gtgacataac ggctgccaga ccaatggctg ccagggcttt
    32341 agtaaatttg tgcatggttc tctccagctt tagtgtcgtt ttgtgtaggg caaaaacgaa
    32401 tgacattcgt taaattaatc ggaaaacaaa gcattacctt ttaactaaaa gataagtgac
    32461 tgtgttgaca tagttttagc gagaaattaa ttctccatag gagagcaata tcacatcgca
    32521 gaattacagt gagaacgtgc ataaatttag cgggaaaaga cataaggaaa agcgaatttg
    32581 tcagacaaat tatcgagtgc acagcagaat aatccataag attagcctgg aagtccttgt
    32641 tgtctttggt acccatgcgg gatgttttct ttttaaccag tcaataggcc tcattacctg
    32701 gcgttgagtt tttgcaatgg tgtaataacc gcaacttaaa gatgtggaaa atgcgcgtca
    32761 ttcatttcgc cattaattat cactgtactc attaattaac agaacacgta taatgagagc
    32821 catatcgcaa aaatgaaaaa acgttttata aaatcattgc ttcatcatga attcaaattc
    32881 atcgat
//
LOCUS       DAZFRE010000055        32332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFRE010000055 DAZFRE010000000
VERSION     DAZFRE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.055"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(146..1906)
                     /gene="ycaO"
                     /locus_tag="ACHKUI_002873"
     CDS             complement(146..1906)
                     /gene="ycaO"
                     /locus_tag="ACHKUI_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6599037.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2036..2728
                     /locus_tag="ACHKUI_002874"
     CDS             2036..2728
                     /locus_tag="ACHKUI_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6599038.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2927..4015
                     /gene="serC"
                     /locus_tag="ACHKUI_002875"
     CDS             2927..4015
                     /gene="serC"
                     /locus_tag="ACHKUI_002875"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6599039.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVVAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4086..5369
                     /gene="aroA"
                     /locus_tag="ACHKUI_002876"
     CDS             4086..5369
                     /gene="aroA"
                     /locus_tag="ACHKUI_002876"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6599040.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIHIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLENMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTMLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            5539..6303
                     /gene="ycaL"
                     /locus_tag="ACHKUI_002877"
     CDS             5539..6303
                     /gene="ycaL"
                     /locus_tag="ACHKUI_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6599041.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6476..7159
                     /gene="cmk"
                     /locus_tag="ACHKUI_002878"
     CDS             6476..7159
                     /gene="cmk"
                     /locus_tag="ACHKUI_002878"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6599042.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7270..8943
                     /gene="rpsA"
                     /locus_tag="ACHKUI_002879"
     CDS             7270..8943
                     /gene="rpsA"
                     /locus_tag="ACHKUI_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6599043.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDIVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9103..9387
                     /gene="ihfB"
                     /locus_tag="ACHKUI_002880"
     CDS             9103..9387
                     /gene="ihfB"
                     /locus_tag="ACHKUI_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6599044.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9593..11857
                     /locus_tag="ACHKUI_002881"
     CDS             9593..11857
                     /locus_tag="ACHKUI_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO6599045.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIVLTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVSKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVIWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11894..13642
                     /gene="msbA"
                     /locus_tag="ACHKUI_002882"
     CDS             11894..13642
                     /gene="msbA"
                     /locus_tag="ACHKUI_002882"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6599046.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13639..14625
                     /gene="lpxK"
                     /locus_tag="ACHKUI_002883"
     CDS             13639..14625
                     /gene="lpxK"
                     /locus_tag="ACHKUI_002883"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6599047.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLLGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            14662..15894
                     /gene="ycaQ"
                     /locus_tag="ACHKUI_002884"
     CDS             14662..15894
                     /gene="ycaQ"
                     /locus_tag="ACHKUI_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6599048.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGDYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYYVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            15946..16128
                     /gene="ycaR"
                     /locus_tag="ACHKUI_002885"
     CDS             15946..16128
                     /gene="ycaR"
                     /locus_tag="ACHKUI_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6599049.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQEIICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16125..16871
                     /gene="kdsB"
                     /locus_tag="ACHKUI_002886"
     CDS             16125..16871
                     /gene="kdsB"
                     /locus_tag="ACHKUI_002886"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6599050.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            17025..17918
                     /locus_tag="ACHKUI_002887"
     CDS             17025..17918
                     /locus_tag="ACHKUI_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6599051.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17895..18674)
                     /gene="elyC"
                     /locus_tag="ACHKUI_002888"
     CDS             complement(17895..18674)
                     /gene="elyC"
                     /locus_tag="ACHKUI_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6599052.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18810..19595
                     /gene="cmoM"
                     /locus_tag="ACHKUI_002889"
     CDS             18810..19595
                     /gene="cmoM"
                     /locus_tag="ACHKUI_002889"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6599053.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19592..20914
                     /gene="mukF"
                     /locus_tag="ACHKUI_002890"
     CDS             19592..20914
                     /gene="mukF"
                     /locus_tag="ACHKUI_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6599054.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20895..21599
                     /gene="mukE"
                     /locus_tag="ACHKUI_002891"
     CDS             20895..21599
                     /gene="mukE"
                     /locus_tag="ACHKUI_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6599055.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21599..26059
                     /gene="mukB"
                     /locus_tag="ACHKUI_002892"
     CDS             21599..26059
                     /gene="mukB"
                     /locus_tag="ACHKUI_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6599056.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVAALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26320..28167
                     /gene="ldtD"
                     /locus_tag="ACHKUI_002893"
     CDS             26320..28167
                     /gene="ldtD"
                     /locus_tag="ACHKUI_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6599057.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28348..28896
                     /locus_tag="ACHKUI_002894"
     CDS             28348..28896
                     /locus_tag="ACHKUI_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6599058.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28923..29570
                     /gene="gloC"
                     /locus_tag="ACHKUI_002895"
     CDS             28923..29570
                     /gene="gloC"
                     /locus_tag="ACHKUI_002895"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6599059.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29620..30810)
                     /gene="aspC"
                     /locus_tag="ACHKUI_002896"
     CDS             complement(29620..30810)
                     /gene="aspC"
                     /locus_tag="ACHKUI_002896"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6599060.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30995..32083)
                     /gene="ompF"
                     /locus_tag="ACHKUI_002897"
     CDS             complement(30995..32083)
                     /gene="ompF"
                     /locus_tag="ACHKUI_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6599061.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7925 a         7831 c         8655 g         7921 t
ORIGIN      
        1 ttattaaata caaattaccg atatttaacc ttataattac aattatttta ttaatgcaaa
       61 tatatgtaaa gcggggcatt aaaaaaacgc cccgtaatat aactcagacg aatcattaag
      121 cctacattgc gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgttgca
      181 gcttttcgta ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta
      241 ctggttgcaa gccataaaat gccgcttcgc cgctcattgc cgcactggcg gcttccacgg
      301 catctgcgcc gtacatgcga acaaacgcat tcagatattg cagcggctgg cgatcttctt
      361 cctgcgccag taataacaac gtttgcaggc agcgataata attggcgcgt tccgggctga
      421 atactgatga gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc
      481 caccagccag cgccagcatg gcttttaatt caccgatacg cagagtgtac cagccgttat
      541 ccgacccggt cgccagaccc aacagttcac gcacgcgggt aaagtcatca aaaccttctt
      601 catccagttg ctcgatgagg ttcaggtaat cttccttttc ccactcgcta cctggtagcg
      661 aaagaatcgt ttcacgtaaa tggctgccca tactgttatt cgccagccac agatcttcag
      721 ccggataaat atcggacatg ccaggcacga taatacggca agcgtagaca cccagatgct
      781 cgtaatcggc aatataaact tctttatctt cctttttgaa gatagccatc agcgtggcga
      841 actcttcttc cgtggtgccg gagaagctcc agtcaacaaa cggataatcg gcatcctgct
      901 tgaacaggtc ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat
      961 gttcagcgac ttcttcatca tcgaaggttg gtggagtaaa cacatccaga tctttcaggc
     1021 cacgaccttg cagcaactcg gtcacggtac gttccagtgc aacgccaaaa tcaggatgcg
     1081 caccgaatga ggcaaagcag gtaccgttag caggattgaa cagtaccacg caaatcaccg
     1141 gatactggcc gccaagcgaa ccgtcataag cgaagatcgg gaaaccttcc gcttccagcg
     1201 tttcgatcgc ttcaactact gctggataac gtgccagcac gtctgccggg atttccggca
     1261 agctgatgct ttcagcaata atgcggtttt tcacgtaacg ttcgaaaact tcggacaacc
     1321 cctgaacgcg tgcttcgtta cgggtattgc cagcagacat accgttagaa acgtacaagt
     1381 taccaatgat attcatcgga atataaatgg tctgattgtc ggactggcgc gtaaacggta
     1441 gaccgcaaat accacgatct tcgttaccgg attgcaggtc aatcagcata ctaccggtca
     1501 gttcattctc cggatcgtaa aatgcgcgca gacggtcatc gagcagccct tctggtacat
     1561 cgtcattttc ggtcagtggg aaccattttt cgttgggata atgcacgaat gggccgttgg
     1621 cgatggtttc gcccagccag aagtccgcaa aaaagtagtt ggttgagaga cgttcgaaat
     1681 attcaccgag ggcagaagcc agcgccgctt ttttggttgc gcctttaccg ttggtaaaac
     1741 acagtgcgca ctctttgtcg cgaatatgta cagaccagac attaggcacg ggattcagcc
     1801 aggaggcctc ttcaatctga aaaccgaggt ctgaaagttt ttgctggaag cgtgcgatgg
     1861 aatcttccag agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag
     1921 tcgtacggaa agcgcgcaat aatacgggtt ttatctcaaa ggtgctatca ccgccgccac
     1981 gctgatgaac tgttagctat gcttttagcg gattaactac gataagagca taaaaatgaa
     2041 ggcgtttgat ctccaccgta tggcattcga taaagtgcct tttgatttcc ttggcgaagt
     2101 tgcactacgt agtctttata cctttgtact ggtctttttg ttcctcaaaa tgaccggaag
     2161 acgcggtgtg cggcagatgt cgctgtttga agtgttaatc attctgacac tgggttcagc
     2221 ggcgggagat gtggcgtttt atgatgatgt gccgatggtc ccggtactta tcgtctttat
     2281 tactctggcg ttgttatacc gcctggtaat gtggttgatg gcgcacagtg aaaaactgga
     2341 agatcttctg gaaggcaagc cggttgtcat tattgaagat ggcgagctgg cctggtcgaa
     2401 actcaataac tccaacatga cggaatttga gttctttatg gagctgcgat tgcgcggtgt
     2461 agagcagttg gggcaggtac gtctggcgat tctcgaaacc aacgggcaaa tcagtgttta
     2521 tttctttgaa gatgacaagg tgaaaccggg gttacttatt ttacccagtg actgtactca
     2581 gcgttacaaa gtggtgccgg agtcggcgga ctatgcctgc atccgttgca gtgaaatcat
     2641 tcatatgaac gcgggggaaa aacaattatg tccgcgctgt gcaaatccag aatggacgaa
     2701 ggcaagtcgg gctaaacggg tgacctgaca gccaaaacat cggttttttg aaaataatcc
     2761 gagagattct tttgtgtgac gcgtgcctca tttttgccct caacggtttt actcattgcg
     2821 atgcgtgtca ctgaatgata aaaccgatag ccacaggaat aatgtattgc ctgtggtcgc
     2881 aattgattga ccgcgggtta atggcaacgc aacgtggtga ggggaaatgg ctcaaatctt
     2941 caattttagt tctggtccgg caatgctacc ggcagaggtg cttaaacagg ctcaacagga
     3001 actgcgcgac tggaacggtc ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga
     3061 gttcattcag gttgcagagg aagccgagaa ggattttcgc gatcttctta acgtcccctc
     3121 caactacaag gtattgttct gccatggcgg tggtcgcggt caatttgctg ctgtaccgct
     3181 gaatattctt ggtgataaaa ccactgcaga ttatgttgat gcgggttact gggcagcaag
     3241 tgctatcaaa gaagcgaaaa aatactgcac accaaatgtg tttgatgcga aagtgaccgt
     3301 tgatggtctt cgcgcagtta agccaatgag tgaatggcaa ctctctgata atgctgctta
     3361 tatgcattac tgcccgaacg aaaccattga cggtatcgct atcgatgaaa caccagactt
     3421 cggtaaagac gtggtggtcg ccgctgactt ctcttcaacc attctttccc gtccgattga
     3481 tgtcagccgt tatggtgtaa tttacgctgg cgcgcagaaa aatatcggcc cggctggcct
     3541 gacaatcgtc atcgttcgtg aagatttgct gggcaaagcg aatatcgcct gcccatcgat
     3601 tctggattac tccatcctca acgataacga ttccatgttc aacacgccgc cgacatttgc
     3661 ctggtactta tctggcctgg tcttcaaatg gctgaaagcg aacggcggtg tggttgcaat
     3721 ggataagatc aatcagcaaa aagcagaact gctgtatggg gtgattgata acagcgattt
     3781 ctaccgcaat gatgtggcga aagctaaccg ttcgcggatg aacgtgccgt tccagttggc
     3841 ggatagcgcg cttgataaat tgttcctcga agagtctttc gctgcgggct tacatgcgct
     3901 gaaaggccac cgagtggtcg gcggaatgcg tgcgtctatt tataacgcca tgccactgga
     3961 aggcgttaaa gcgctgacag acttcatggt tgagttcgaa cgccgtcacg gctaatgtcg
     4021 aaattttgca taatccccac agccagcctg tggggttttt atttctgttg tagagagttg
     4081 agttcatgga atccctaacg ttacaaccca tcgctcgtgt cgatggcact attaatctgc
     4141 ccggttccaa gagcgtttct aaccgcgctt tattgctggc ggcattagca cacggcaaaa
     4201 cagtattaac caatctgctg gatagcgatg acgtgcgcca tatgctgaat gcattaacag
     4261 cgttaggggt aagctatacg ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg
     4321 gtggtccatt acacgcagaa agtgcccgtg agttgttcct cggtaacgcc ggaacggcaa
     4381 tgcgtccgct ggcggcagca ctttgcctgg gtagcaatga tattgtactg accggtgaac
     4441 cgcgaatgaa agaacgcccg attggtcatc tggtggatgc gctgcgccag ggcggggcga
     4501 agatcactta cctggaacaa gaaaattatc cgccattgcg tttacagggc ggctttactg
     4561 gcggcaacgt tgatgttgat ggctccgttt ccagccagtt cctcaccgca ctgttaatga
     4621 ctgcgcctct tgcgccggaa gatacggtga ttcatattaa aggcgatctg gtttctaaac
     4681 cttatatcga tatcaccctc aatctgatga agacgtttgg tgttgaaatt gaaaatcagc
     4741 actatcaaca atttgtcgta aaaggggggc agtcttatca gtctccgggt acttatttgg
     4801 tcgaaggcga tgcatcctcg gcttcttatt tcctggcagc agcagcaatc agaggcggca
     4861 ctgtaaaagt gaccggcatt ggacgcaaca gtatgcaggg tgatattcgc tttgctgatg
     4921 ttctggaaaa tatgggtgcg accatttgct ggggcgatga ttatatttcc tgcacgcgtg
     4981 gtgaactgaa cgccattgat atggatatga accatattcc cgatgcggcg atgaccattg
     5041 ccaccgcggc gttatttgca aaaggcacca ccatgctgcg caatatctat aactggcgtg
     5101 ttaaagagac cgatcgcctg tttgcgatgg caacagaact gcgtaaagtc ggtgcggaag
     5161 tcgaagaggg gcacgatttt attcgcatta cgccaccgga aaaactgaaa tttgctgaga
     5221 tcgccactta taatgatcac cggatggcga tgtgtttctc gctggtcgca ttatcagata
     5281 cgcctgtgac gattcttgat ccaaaatgta ccgctaaaac ttttccagac tatttcgagc
     5341 aattggcgcg gataagtcaa cccggctgaa tgaataacgg gcgataacat agacaatcct
     5401 ttcttttcaa agacgtcggc acattgccgg cgtttttttt cggaccttgt ggggtatttt
     5461 tattaatggt agcgttaatt atcaatgtaa cttgttgata cataatgttt atatataatt
     5521 aatcaacgga tgattcacat gaagaatact aaattattgc tggcgattgc gacctctgca
     5581 gccttactga caggatgtca gaatacgcac ggtattaata ccgatttggc tatcagctca
     5641 ggtctgaatg cttataaagc cgcaacatta agcgatgctg atgctaaagc aatcgccaat
     5701 cagggctgtg tcgaaatgga cagcggcaat aaagtggcaa gcaaatccag caagtacggg
     5761 aaacgtctgg caaaaatcgc caaagcattg ggtaacaata ttaatggcac gccagtcaac
     5821 tataaggttt atatgaccag cgacgtcaac gcatgggcga tggcgaacgg ttgtgttcgt
     5881 gtctacagtg gcctgatgga catgatgaac gacaacgaaa ttgaaggcgt tctgggccat
     5941 gaactgggcc acgtcgcgtt gggtcactcg ctggctgaaa tgaaagcttc ttatgcgatc
     6001 gtggccgcac gcgatgccat ttcagctacc agcggtgtgg cttcccagct ttcccgttca
     6061 caattaggtg atatcgcaga aggcgctatc aatgctaaat actcccgtga taaagaatcc
     6121 gaagcagatg atttctcttt tgatctgttg aagaaacgtg gcatcagtac ccaggggctg
     6181 gttggtagct ttgaaaaact ggccagcctg gatggcggtc gcacccagtc catgtttgat
     6241 tctcacccac catcaacaga gcgtgcgcaa cacatccgtg atcgtatcgc ctctggtaag
     6301 taaatcattg tcatctttcg ggctggtctt ctgcctgccc gctataattg cgcaataaat
     6361 ccccatctga atacagacaa aactggtttt tgcacacaac gttaacgatt tgtggcgtcg
     6421 gcgcgtataa tgcgcgcggt tatgttaacg gtacgcctgt tttaaggaga taaagatgac
     6481 ggcaattgcc ccggttatta ccattgatgg cccaagcggt gcagggaaag gcaccttgtg
     6541 taaggctatg gcggaagcgt tgcaatggca tctgctggat tcgggtgcaa tttatcgcgt
     6601 actggcacta gcggcattac atcaccatgt tgatgttgca tcggaagatg cgctggtgcc
     6661 gctggcatcc catctggatg tacgttttgt gtcaaccaac ggcaatctgg aagtgatcct
     6721 cgaaggggaa gatgtcagcg gcgaaattcg tactcaggag gtggcgaatg ctgcttcaca
     6781 agtcgcggca ttcccacgcg ttcgtgaagc attattgcgt cgccaacgcg cgtttcgcga
     6841 attaccaggc ctgattgccg atgggcgcga catgggaacg gtggtattcc ctgatgcgcc
     6901 agtgaaaatt ttccttgacg cctcctcgga agaacgtgcg catcgccgca tgctacagtt
     6961 gcaggagaag ggctttagtg ttaactttga gcgccttttg gccgagatca aagaacgtga
     7021 cgaccgcgat cgtaatcgag cggtagcgcc actggttccg gcagccgatg ctttagtgtt
     7081 ggattccacc accttaagca ttgagcaagt gattgaaaaa gcgctacaat acgcgcgcca
     7141 gaaattggct ctcgcataag cgaccgaatt tgcagtaccc ccgttgcaat ggaatgacag
     7201 cgggtatgtt aaacaacccc atccggcatg gagccaggtg gacgttaaat ataaacctga
     7261 agattaaaca tgactgaatc ttttgctcaa ctctttgaag agtccttaaa agaaatcgaa
     7321 acccgcccgg gttctatcgt tcgtggcgtt gttgttgcta tcgacaaaga cgtagtactg
     7381 gttgacgctg gtctgaaatc tgagtccgcc atcccggctg agcagttcaa aaacgcccag
     7441 ggcgagctgg aaatccaggt aggtgacgaa gttgacgttg ctctggacgc agtagaagac
     7501 ggcttcggtg aaactctgct gtcccgtgag aaagctaaac gtcacgaagc ctggatcacg
     7561 ctggaaaaag cttacgaaga tgctgaaact gttaccggtg ttatcaacgg caaagttaag
     7621 ggcggcttca ctgttgagct gaacggtatt cgtgcgttcc tgccaggttc tctggtagac
     7681 gttcgtccgg tgcgtgacac tctgcacctg gaaggcaaag agcttgaatt caaagtaatc
     7741 aagctggatc agaagcgcaa caacgttgtt gtttctcgtc gtgccgttat cgaatccgaa
     7801 aacagcgcag agcgcgatca gctgctggaa aacctgcagg aaggcatgga agttaaaggt
     7861 atcgttaaga acctcactga ctacggtgca ttcgttgatc tgggcggcgt tgacggcctg
     7921 ctgcacatca ctgacatggc ctggaaacgc gttaagcatc cgagcgaaat cgtcaacgtg
     7981 ggcgacgaaa tcactgttaa agtgctgaag ttcgaccgcg aacgtacccg tgtatccctg
     8041 ggcctgaaac agctgggcga agatccgtgg gtagctatcg ctaaacgtta tccggaaggt
     8101 accaaactga ccggtcgcgt gaccaacctg accgactacg gctgcttcgt tgaaatcgaa
     8161 gaaggcgttg aaggcctggt acacgtttcc gaaatggatt ggaccaacaa aaacatccac
     8221 ccgtccaaag ttgttaacgt tggcgatata gtggaagtta tggttctgga tatcgacgaa
     8281 gaacgtcgtc gtatctccct gggtctgaaa cagtgcaaag ctaacccgtg gcagcagttc
     8341 gcggaaaccc acaacaaggg cgaccgtgtt gaaggtaaaa tcaagtctat cactgacttc
     8401 ggtatcttca tcggcctgga tggcggcatc gacggcctgg ttcacctgtc tgacatctcc
     8461 tggaacgttg caggcgaaga agcagttcgt gaatacaaaa aaggcgacga aatcgctgca
     8521 gttgttctgc aggttgacgc agaacgtgaa cgtatctccc tgggcgttaa acagctcgca
     8581 gaagatccgt tcaacaactg ggttgctctg aacaagaaag gcgctatcgt aaccggtaaa
     8641 gtaactgcag ttgacgctaa aggcgcaacc gtagaactgg ctgacggcgt tgaaggttac
     8701 ctgcgtgctt ctgaagcatc ccgtgaccgc gttgaagacg ctaccctggt tctgagcgtt
     8761 ggcgacgaag ttgaagctaa attcaccggc gttgatcgta aaaaccgcgc aatcagcctg
     8821 tctgttcgtg cgaaagacga agctgacgag aaagatgcaa tcgcaactgt taacaaacag
     8881 gaagatgcaa acttctccaa caacgcaatg gctgaagctt tcaaagcagc taaaggcgag
     8941 taattctctg actcttcggg atttttattc cgaagtttgt tgagtttact tgacagattg
     9001 caggtttcgt cctgtaatca agcactaagg gcggctacgg ccgcccttaa tcaatgcagc
     9061 aacagcagcc gcttaatttg cctttaagga accggaggaa tcatgaccaa gtcagaattg
     9121 atagaaagac ttgccaccca gcaatcgcac attcccgcca agacggttga agatgcagta
     9181 aaagagatgc tggagcatat ggcctcgact cttgcgcagg gcgagcgtat tgaaatccgc
     9241 ggtttcggca gtttctcttt gcactaccgc gcaccacgta ccggacgtaa tccgaagact
     9301 ggcgataaag tagaactgga aggaaaatac gttcctcact ttaaaccagg taaagaactg
     9361 cgcgatcgcg ccaatattta cggttaagtt tttactcaaa cttgaacgcg agaaaagcac
     9421 ctgtcgggtg cttttttcat ttctctaatc tggaactgga agctgcctcg cacagttttg
     9481 agcagttttc accctatcgt taaattcttc tgaacgtgcc tcggggaacg caaaactccc
     9541 accagacaac cgttcaacaa agttgcacac tttctataaa cagggagggt gcatgaaaat
     9601 aacgacagtc ggcgtatgca taatttgcgg aatttttccg ttgctgattt tgccccaatt
     9661 gcctgggaca gtaacccttg cgtttctgac tctcttcgcc tgtgtactgg catttatccc
     9721 tgttaaaacc gtccgttata tcgtgctgac gttgctgttt ttcgtttggg gcatattagc
     9781 agcaaagcaa attttgtggg caggagaaac cttaactggc gcgacgcagg atgcaattgt
     9841 tgagatcatt gctactgacg gcatgaccac tcattacggt caaattactc atctacaagg
     9901 tcgacgtata ttccctgcgc caggcctcgt actgtatggc gaatatcttc cgcaagcggt
     9961 ttgtgccgga caagtatggt caatgaaact caaagttcgt gcagttcatg gtcaacttaa
    10021 tgatggcggc tttgatagcc agcgttatgc cattgcccag catcaaccgc tcaccggccg
    10081 ttttctgcag gcaagtgtca ttgaaccgaa ttgtagcctg cgtgcacagt atctggcgtc
    10141 actacaaaca acgctgcaac cctatatgtg gaacgcggtt attcttggat taggtatggg
    10201 ggaacggtta tccgtctcca aagaaatcaa aaatatcatg cgtgatactg gaacggcgca
    10261 tttaatggcg atatcaggat tgcacatcgc ttttgcggcg ttgctggctg caggactcat
    10321 tcgcggtggg caagtttttc tgcctgggtg ctggatccac tggcaaatgc cattaattgg
    10381 tggaatctgc tgtgctgctt tttatgcctg gcttacggga atgcaacctc ctgcattgcg
    10441 taccgtggtg gcgcttgcca tttggggaat gcttaagtta agtggacgac aatggagtgg
    10501 ctgggatgta tggatatgtt gtctggcggc aattttgctg atggatcctg ttgccattct
    10561 ctcgcaaagt ttatggctct ctgccgctgc ggtcgcggca ctgatttttt ggtatcagtg
    10621 gtttccctgt ccggagtggc agctgccgcc ggtattgcgc gcggttgttt ccctcatcca
    10681 tctgcaactg ggaatcaccc tcctgcttat gcccgtgcaa attgtcatat ttcatggcat
    10741 tagtctgacc tcgtttattg caaatctttt tgcaattccc ctggtgacat ttatcacggt
    10801 tccgttgatt ctcgccgcga tggttgtgca tttaagcggg ccgtttattc tggaacaagg
    10861 gttatggttt cttgccgacc ggtctttggc tttacttttc tgggggttaa agagtttgcc
    10921 ggaagggtgg atcaacattg ctgaacgttg gcaatggcta acattttccc catggttctt
    10981 actggtgata tggcgattaa acgcctggcg aacgttgcca gcaatgtgtg tggctgtagg
    11041 cttgctgatg tgcttgccgc tgtggcaaaa acctcgacct gacgagtggc aagtgtacat
    11101 gcttgatgtc gggcaagggc tggcaatggt cattgcgcga aacggcaaag cgattctcta
    11161 tgatacggga ctggcctggc ccgaagggga tagtgggcaa caactgatta tcccctggct
    11221 ccactggcat aatcttgaac cggaaggcgt tattttgagt catgaacatc tggatcaccg
    11281 gggagggctg gactcaatat tgcacacatg gccgatgtta tggatccgaa gtccgttaaa
    11341 ctgggaacat catcagccct gtgtgcgtgg cgaagcgtgg caatggcaag gattgcgttt
    11401 cagcgcgcac tggcctttac aaggtagcaa cgataaagga aataaccatt cctgtgtggt
    11461 taagattgat gacgggacga atagcattct tctaaccggt gatattgaag cccccgctga
    11521 acaaaagatg ctaagccgtt actggcagca aatgcaggca acattacttc aggtacctca
    11581 ccatggcagt aatacctcat catcgttgcc attaattcag cgagtgaatg gaaaagtggc
    11641 actcgcatcg gcatcgcgct ataacgcatg gcgactgccc tcaagcaaag ttaaacatcg
    11701 ctatcaacaa cagggttata agtggcttga tactccacat cagggtcaaa taacggtcaa
    11761 tttttcagcg caaggctggc ggattagcag cctcagagag caaattttac ctcgttggta
    11821 tcatcagtgg tttggcgtgc cagtggataa cgggtagaat atgcggctat ttcaacaaat
    11881 gctggttttt tgaatgcata acgacaaaga tctctctacg tggcagacgt tccgccgact
    11941 gtggccaacc attgcgcctt ttaaagcggg tctgatcgtg gcgggcgtag cgttaatcct
    12001 caacgcagcc agcgatacct tcatgttatc gctccttaag ccacttcttg atgatggctt
    12061 tggtaaaaca gatcgctccg tgctgatgtg gatgccgctg gtggtgatcg ggctgatgat
    12121 tttacgtggt atcaccagct atatctccag ctactgtatc tcctgggtat caggaaaggt
    12181 ggtaatgact atgcgtcgcc gcctgtttgg tcacatgatg ggaatgccgg tttcattctt
    12241 tgacaaacag tcaacgggta cgctgttgtc acgtatcacc tacgattccg aacaggtcgc
    12301 ttcttcttct tccggcgcac tgattactgt tgtgcgtgaa ggtgcgtcga tcatcggcct
    12361 gttcatcatg atgttctatt acagttggca actgtcgatc attttgattg tgctggcacc
    12421 gattgtttcg attgcgattc gcgttgtatc gaagcgtttt cgcaacatca gtaaaaacat
    12481 gcagaacacc atggggcagg tgaccaccag cgccgaacaa atgctgaaag gccataaaga
    12541 agtattgatt ttcggtggtc aggaagtgga aacgaaacgc ttcgataaag tcagcaaccg
    12601 aatgcgtctt caggggatga aaatggtttc agcctcttct atctctgatc cgatcattca
    12661 gctgatcgcc tctttggcgc tggcgtttgt tctgtatgcg gcaagcttcc caagtgtcat
    12721 ggatagcctg actgccggta cgattaccgt tgttttctct tcaatgattg cacttatgcg
    12781 tccgctgaaa tcgctgacca acgttaacgc ccagttccag cgcggtatgg cggcttgtca
    12841 gacgctgttt accattctgg acagtgagca ggagaaagac gaaggtaagc gcgtgatcga
    12901 gcgtgcgact ggcgacgtgg agtttcgcaa tgtcaccttt acttatccgg gacgtgatgt
    12961 gcctgcattg cgtaacatca acctgaaaat tccggcaggg aagacggttg ctctggttgg
    13021 acgatctggt tcaggtaaat caaccatcgc cagcctaatc acacgttttt acgatattga
    13081 tgaaggcgaa atcctgatgg atggtcacga tctgcgcgag tataccctgg cgtcgttacg
    13141 taaccaggtt gctctggtgt cgcagaatgt ccatctgttt aacgatacgg ttgccaacaa
    13201 cattgcttac gcaaggactg aacagtacag ccgtgagcaa attgaagaag ctgcgcgtat
    13261 ggcctacgcc atggacttca tcaataagat ggataacggt ctcgatacag tcattggtga
    13321 aaacggcgtg ttgctctctg gcggtcagcg tcagcgtatt gctatcgctc gagccttgtt
    13381 gcgtgatagc ccgatactga ttctggacga agctacctcg gcgttggata ccgaatccga
    13441 acgtgcgatt caggcggcac tggatgagtt gcagaaaaac cgtacctctc tggtgattgc
    13501 ccaccgcttg tctaccattg aaaaggcaga cgaaatcgtg gttgtcgagg atggtgtcat
    13561 tgtggaacgc ggtacgcata acgatttgct tgagcaccgt ggcgtttacg cgcaacttca
    13621 caaaatgcag tttggccaat gatcgaaaaa atctggtctg gtgaatcccc tttatggcgg
    13681 ctattgctgc cactctcctg gttgtatggc cttgtgagtg gcgcgatccg tctttgctat
    13741 aaactaaaac tgaagcgcgc ctggcgtgcc cccgtaccgg ttgtcgtggt tggtaatctc
    13801 accgcaggcg gcaacggaaa aaccccggtc gttgtctggc tggtggaaca gctgcaacag
    13861 cgcggtattc gcgtgggggt tgtatcgcgg ggatatggtg gtaaggctga atcttatccg
    13921 ctgttattgt cggcagatac caccacagca caggcgggtg atgaacctgt gttgatttat
    13981 caacgcactg gcgcgcctgt tgcggtttct cccgtgcgtt ctgacgcggt aaaagccatt
    14041 ctggcgcaac accctgatgt gcagatcatc gtaaccgacg atggtttaca gcattatcgt
    14101 ctggcgcgtg atgtagaaat tgtcgttatt gatggtgtgc gtcgctttgg caatggctgg
    14161 tggttgccgg cggggccaat gcgtgagcga gcggggcgct taaagtcggt tgatgcggta
    14221 atcgtcaacg gcggtgtccc ccgcagcggt gaaatcccca tgcatctgct gctgggtcag
    14281 gcggtgaatt tacgtaccgg tacgcgttgt gacgttgctc agcttgaaca tgtggtggcg
    14341 atggcaggga ttgggcatcc gccgcgcttt tttgccacgc tgaagatgtg cggcgtacaa
    14401 ccggaaaaat gtgtaccgct ggccgatcat cagtctttga atcatgcgga tgtcagcgcg
    14461 ttggtaagcg ccggacaaac gctggtaatg actgaaaaag atgcggtgaa atgccgggcc
    14521 tttgcagaag aaaattggtg gtatttgcct gtagacgcac agctttcagg tgatgaacca
    14581 gcgaaactgc ttgcgcaact aacctcgctg gcttctggcc actagttacg ccgcggcagc
    14641 gttcgattga tggagtcatg aatgtcgctg ccgcacctct cccttgctga tgcgcgtaat
    14701 cttcaccttg ccgcgcaagg cctgttaaac aaaccccgcc gtcgagcgtc gttggaggat
    14761 attccggcaa cgatctcccg catgtccttg ctgcaaatcg ataccatcaa tattgttgcc
    14821 cgtagcccat atctggtgct tttcagccgt ctgggagatt atcctgcgca gtggctggat
    14881 gagtctctgg cgcgtggcga attaatggaa tactgggcgc atgaagcctg ctttatgccg
    14941 cgtagcgatt ttcgtcttat tcgccaccgc atgctggcac ctgaaaaaat gggctggaaa
    15001 tataaagacg cctggatgca ggaacatgcg gcggaaattg cactgttaat tcagcatatt
    15061 catgataagg ggccggtacg ttcagccgat tttgagcatt ctcgtaaagg ggcgagcggc
    15121 tggtgggagt ggaagccgca taaacgacat ctggaaggtt tatttactgc cggaaaggtg
    15181 atggtgattg aacggcgcaa cttccagcgc gtttatgatt taactcaccg tgtcatgcct
    15241 gactgggatg atgagcgcga tctcgtttcg caagcagaag cagaaatcat catgctggat
    15301 aacagtgcgc gtagcctggg aatatttcgc gaacagtggc tggcagatta ctaccggcta
    15361 aaacgtccgg cactggcggc gtggcgtgaa gcgagggctg aacagcagca aatcattgct
    15421 gtgcatgttg aaaaattggg caatctttgg ctgcatgctg atttgctgcc gctactcgag
    15481 cgtgcgctcg ccggaaagct cactgcaacg cacagcgcgg tactttcgcc ttttgatcct
    15541 gttgtctggg atcgcaaacg cgcggagcag ctttttgatt ttagctaccg gctggagtgc
    15601 tatatcccag cgccgaaacg ccagtatggc tattatgttc tgccgttatt acatcgtggg
    15661 caattagttg ggcgaatgga tgccaaaatg catcgccaga caggcatcct tgaagttatc
    15721 tctctgtggt tacaggaagg cattaaacca acgacaacgc tgcaaaaagg gttacgtcag
    15781 gcgattactg atttcgctaa ctggcagcag gcaacgcggg tgacattagg acgctgcccg
    15841 caaggcctct ttactgattg ccgcgccggc tgggaaatag accccgtcgc ataaatgatt
    15901 atgataagat ttatgcattc ataacccgat ccatctggag gaactatgga tcatcgtctg
    15961 cttgaaatca ttgcctgccc ggtttgcaac ggaaaacttt ggtataacca ggaaaaacaa
    16021 gagattattt gcaaactgga caacctggct ttcccgctgc gcgatggcat tccagtgttg
    16081 ctggaaacgg aagcccgcgt gctgactgct gatgagagta aatcatgagt tttgtggtca
    16141 ttattcccgc gcgttacgcg tccacgcgtc tgcccggtaa accattggtt gatattaacg
    16201 gcaaacccat gattgttcat gttcttgaac gcgcgcgcga atcaggtgcc gagcgcatca
    16261 tcgtggcaac cgatcatgag gatgttgccc gcgctgttga agccgctggc ggcgaagtat
    16321 gtatgacgcg cgccgatcat cagtcaggta cagaacgatt ggcggaagtt gtcgaaaaat
    16381 gcgcattcag cgacgacact gtgatcgtta atgtgcaggg tgatgaaccg atgatccctg
    16441 cgacaatcat tcgtcaggtt gctgataacc tcgctcagcg tcaggtgggt atggcgactc
    16501 tggcggtgcc aatccacaat gcggaagaag cgtttaaccc gaatgcggtg aaagtggttc
    16561 tcgacgccga agggtacgca ctgtacttct ctcgcgccac cattccgtgg gatcgtgatc
    16621 gttttgcaaa agaccttgaa accgtaggcg ataacttcct gcgtcatctt ggtatttatg
    16681 gctaccgagc aggctttatc cgtcgttatg ttacctggca gccaagtccg ttagaacata
    16741 tcgaaatgtt agagcagctt cgtgttctgt ggtacggcga aaaaatccat gttgctgtcg
    16801 cccaggaagt tcctggcaca ggtgtggata cccctgaaga tctcgagcgc gttcgcgctg
    16861 aaatgcgcta atttcacttc acgacactcc agccaatttt gggaggagtg tcgtaccgtt
    16921 acgattttcc tcaatttttc ttttcaacaa ttgatctcat tcaggtgaca tcttttatat
    16981 tggcgctcat tatgaaagca gtagctttta tgagggtaat ctgaatggaa cagctgcgtg
    17041 ccgaattaag ccatttactg ggcgaaaaac tcagtcgtat tgagtgcgtc aatgaaaaag
    17101 cggatacggc gttgtgggct ttgtatgaca gccagggaaa cccaatgccg ttaatggcaa
    17161 gaagctttag tacgcccgga aaagcccgac aactggcatg gaaaaccacc atgctggcaa
    17221 gaagtgggac tgtccgtatg ccgactattt atggtgtgat gacgcatgaa gaacaccccg
    17281 gccccgatgt cctgctactg gagcgaatgc gtggtgtttc ggtggaggca ccagcccgaa
    17341 caccagaacg ctgggaacaa ctcaaagatc aaatcgttga agccttactg gcctggcacc
    17401 gtcaggacag tcgcggttgc gtcggcgcgg tcgacaatac tcaggaaaat ttctggccct
    17461 catggtaccg gcaacatgtt gaagtgctat ggaccacgct caatcagttc aataacaccg
    17521 gtctaacgat gcaggataag cggatcctgt ttcgcactcg cgaatgtctc ccggcattat
    17581 ttgaaggctt taacgacaat tgtgtactga ttcacggtaa cttctgttta cgtagcatgc
    17641 tgaaagattc gcgcagcgat cagttactgg cgatggtcgg accgggacta atgctttggg
    17701 caccgcgaga atacgaactg ttccgactaa tggataattc tctggcggaa gatttgctct
    17761 ggagttatct gcaacgcgcg ccagtggcgg agtcgttcat ctggcggcgt tggttgtatg
    17821 tgttatggga tgaagttgcg caactggtca acaccggacg atttagtcgg cgtaacttcg
    17881 atctggcatc aaaatcactc ttgccgtggc tcgcctgacg aaccttttag ccactgccag
    17941 atgcggccaa gcgtttcata gccaacacga tcgctatgca tcaaccaaac cggagaaggg
    18001 attgcccgtt cccacggatt aagcggcgag tcgatggcca actgattcgc cggggctggc
    18061 agcggattta gcccttcctg ctgaaaaaag atcatcgccc gcggcagatg tgatgcggag
    18121 gtcaccagca ggaaaggggc gtcaccaatt gcctgtttca ctgccgcagc ttcttcttcg
    18181 gtatcttttg gcaaatccag agtgataatt tgctcgcgcg gcacgcccag cgattgcgca
    18241 actcttgcac ctacttcggc tgtactcacc gtattggttt ttgctacgcc tcccgtgaag
    18301 atcagttttg atcccggatt ttcgcgccat aagcgaatac cttcattcag gcggggcaga
    18361 ctgttattga ttaaattcga gcttggtgcc cactgcgggt tccaggtata accacctccg
    18421 agcaccacga tatagtccac tttttgcgaa ttattccatg tcggataggt gctttcgatg
    18481 ggacgcagca ggcggtcggc taccggttgc aaactcaata acaagagcgc cagccaccca
    18541 atgctaataa aaatcttccc ggttttttga aaacggctaa accacagcag cgccaggcca
    18601 gcgccaataa tcaacaacat cagcggaagt ggcagcaaca tattgccaat cacttttttc
    18661 agtgtaaaaa gcatcctttc tggttccttt tttgaccata tagcaaggga tcgccagcga
    18721 tattacacca gacgggttca ttctcgccgt ggctgtgaca aaatagcgtc tttatcagaa
    18781 aacgtcttag ccaaccggtg gagaaagcaa tgcaggatcg caattttgat gatattgcgg
    18841 aaaagttttc ccgtaacatt tacggcacca ccaaagggca gcttcgacag gctattctgt
    18901 ggcaggatct cgatcgcgtg ctggcggaaa tgggcccgca aaaactgcgt gtgctggatg
    18961 ctggcggcgg agaagggcag accgcaatca aaatggccga gcgcgggcat caggtcattt
    19021 tatgcgatct ttctgcgcag atgatcgacc gcgcaaaaca ggcggcagaa gcaaaaggtg
    19081 tgagcgacaa catgcaattt atacattgcg ccgctcagga tgttgcttcg catttggaaa
    19141 cgcccgttga tctgatattg ttccatgcgg tgctggagtg ggtggccgat ccccgcagcg
    19201 tattacagac cctgtggtca gttttgcgtc caggcggcgt gttgtcgtta atgttctaca
    19261 atgcgcatgg tttgttgatg cataacatgg tcgccgggaa ttttgattac gtgcaggcgg
    19321 gaatgccgaa aaagaaaaaa cggacgcttt cgccagatta tccacgcgac ccggcgcagg
    19381 tttatctgtg gctggaagaa gctggttggc aaattatggg taagacaggc gttcgcgtgt
    19441 ttcatgatta tctgcgcgag aagcaccagc agcgcgactg ctatgaagca ttacttgaat
    19501 tagaaacgcg ttattgccgt caggaaccgt atattaccct ggggcgttat attcatgtca
    19561 ccgcgcgcaa accgcagagc aaggataaag tatgagtgaa ttttcccaga cagtccccga
    19621 actggttgcc tgggccagaa aaaatgactt ctccatctcg ctgccggtag accgactctc
    19681 ttttctgctg gcggttgcca cgctgaacgg cgagcgtctg gatggtgaga tgagtgaagg
    19741 cgagctggtg gatgcattcc gccatgtgag tgatgcgttt gagcaaacca gcgaaaccat
    19801 cggcgtgcgc gccaacaacg cgatcaacga catggtgcgt caacgtctgc tgaaccgctt
    19861 taccagcgag caggcggaag ggaacgcaat ttaccgtctg acgccgctcg gcatcggcat
    19921 cactgactat tacattcgtc agcgcgagtt ttctacgctg cgcctttcta tgcagttgtc
    19981 gattgtggcg ggtgagctca aacgcgcagc agatgccgcc gaagagggcg gtgatgaatt
    20041 tcactggcac cgtaatgttt atgcgccact gaaatattcg gtagcagaaa ttttcgacag
    20101 tatcgacctg acgcaacgtc tgatggatga acagcagcag caggtgaagg atgatatcgc
    20161 ccagttgttg aacaaagact ggcgggcggc gatttccagc tgtgaattgt tgctttcaga
    20221 aacttccgga acgctgcgtg aattgcagga tacgctggaa gcggcaggcg ataaattgca
    20281 ggccaacctg ttacgcattc aggatgcgac gatgacccat gacgacctgc attttgtcga
    20341 tcgtctggtg ttcgatcttc agagcaaact cgaccgtatt atcagttggg gccagcaatc
    20401 catcgacttg tggattggct acgaccgcca cgtacacaaa tttattcgta ctgcgatcga
    20461 tatggataaa aaccgcgtct ttgctcagcg gttacgtcag tcggtacaaa cctattttga
    20521 tgagccgtgg gcgctaacct atgccaatgc cgatcgtctg ctggatatgc gtgacgaaga
    20581 gatggtactg cgcgatgaag aagtgactgg ggaacttcct gaggatctgg agtacgaaga
    20641 gtttaacgag atccgcgaac agctggcggc gatcatcgaa gaacaacttg ccgtgtacaa
    20701 aaccagacaa gtgccgctgg atcttggtct ggtggtacgc gaatatctgt cacagtatcc
    20761 gcgtgcacgt cactttgacg ttgcgcgcat tgttattgat caggcggtac gtcttggcgt
    20821 agcgcaagca gatttcaccg gactgccagc gaaatggcag ccgattaatg attacggagc
    20881 caaggtacag gcgcatgtca tcgacaaata ttgaacaagt gatgccggtt aagctggcgc
    20941 aggcgctggc gaatccgtta tttccggcgc tggatagcgc cttacgttca ggccgccata
    21001 ttggcctcga cgaactggat aatcatgcat tcctgatgga ttttcaggaa tatctggaag
    21061 agttttacgc gcgttataac gttgagctta ttcgtgcacc ggaagggttc ttctatctac
    21121 gcccacgttc caccacgctg atccctcgtt ccgtcttgtc ggaactggat atgatggtcg
    21181 ggaaaatcct ctgttatctc tatctcagcc cggaacggct ggcgaatgag gggattttta
    21241 ctcaacagga actgtacgac gaactgctca ccctggccga tgaagcgaaa ctgctgaaac
    21301 tggtgaacaa ccgttcaacc ggttcagacg ttgaccgtca gaagttgcag gaaaaagtac
    21361 gctcttcgct caaccgtctg cgtcgtttag gcatggtgtg gtttatgggc cacgacagca
    21421 gcaagttccg cattaccgaa tcggtgttcc gcttcggagc cgatgtgcgt gctggcgacg
    21481 atccccgtga agcacagcgt cgcctgattc gtgatggtga agcaatgccg attgaaaacc
    21541 atctgcaact caacgatgaa accgaagaga gtcagccaga tagcggagag gaagaataat
    21601 gattgaacgc ggtaaatttc gctcactgac gctgattaac tggaacggct tttttgcccg
    21661 aacttttgac cttgacgagc tggtcacgac gctttctggc ggtaacgggg cgggtaaatc
    21721 caccaccatg gcggcgttcg ttacggcgct tatccccgat ctgaccctgc tgcatttccg
    21781 taacactacg gaagccgggg ccaccagcgg ttcgcgcgat aaaggtctgc acggtaagct
    21841 gaaagcgggt gtctgttatt cgatgcttga caccattaac tcgcgccacc agcgtgtagt
    21901 ggtcggtgtg cgtctgcaac aggttgccgg acgcgatcgt aaagtggata tcaaaccatt
    21961 tgccattcag ggcctgccga tgtcggtgca gccgacacaa ctggtgaccg aaactctgaa
    22021 cgaacgccag gcgcgcgtgc tgccgcttaa cgagctgaaa gacaagctcg aggcgatgga
    22081 aggcgtgcag tttaaacagt tcaactccat taccgattac cactcgctga tgttcgatct
    22141 gggcatcatc gcgcgtcgtc tgcgctcagc gtctgaccgt agtaaattct atcgtctgat
    22201 cgaagcctcg ctgtatggcg ggatctccag cgccattact cgttctctgc gcgactacct
    22261 gttgccggaa aacagtgggg tgcggaaagc gttccaggac atggaagcgg cgctgcgtga
    22321 aaaccgcatg acgctggaag cgattcgtgt cacccagtcg gaccgagacc tgtttaagca
    22381 tctgatcagc gaagccacca actacgtggc ggcggactac atgcgtcacg ccaacgagcg
    22441 ccgtgttcat ctcgataagg ccctggagtt tcgtcgcgag ctacatactt cgcgtcagca
    22501 actggcggct gagcagtaca aacacgtcga tatggcgcga gagctggctg agcacaacgg
    22561 tgccgaaggc gatctggaag cggattatca ggcggccagc gatcacctga acctggtgca
    22621 aaccgcactg cgtcagcagg agaagatcga acgctacgaa gcggatctcg atgagctgca
    22681 gatccgtctg gaagagcaaa acgaagtggt ggcagaagcc atcgaacgcc aggaagagaa
    22741 cgaggctcgt gcggaagctg ccgaactgga agtggacgag ctgaaaagcc agcttgctga
    22801 ctaccagcag gcgctggacg tccagcaaac gcgcgcgatc cagtataacc aggcgattgc
    22861 tgcgcttaat cgtgccaaag aactgtgcca tctgccggac ttaaccgccg acagcgctgc
    22921 cgaatggctg gaaaccttcc aggcgaaaga gctggaagcg accgaaaaaa tgctctctct
    22981 tgagcagaaa atgagcatgg cgcaaaccgc gcacagccag tttgagcagg cttatcagct
    23041 ggtggtggca atcaacggcc cgctggcgcg taacgaggcg tgggatgtcg ctcgcgaact
    23101 attgcgcgaa ggggtcgatc agcgtcacct ggcagagcag gttcagccgt tgcggatgcg
    23161 cttaagcgaa ctggaacagc gtctgcgtga gcagcaagaa gctgagcgtc tgctggcaga
    23221 tttctgcaaa cgtcagggca agaattttga tattgatgaa ctggaagctc tgcatcagga
    23281 gctggaagca cgcattgcct ctctttccga tagcgtctct aacgcccgag aagagcggat
    23341 ggcactgcgc caggagcagg aacagctgca gtctcgcatt cagagtttga tgcagcgtgc
    23401 gccggtttgg ctggcagcgc aaaacagtct caaccagttg agcgaacagt gcggcgaaga
    23461 gttcacctcc agccaggatg tcaccgaatt tctgcaacag ttgctggagc gcgagcgcga
    23521 ggcaattgtt gaacgcgatg aagtaggcgc gcgcaaaaac gccgtcgatg aagagatcga
    23581 acgtttaagc cagccaggtg gttctgaaga tcagcgtctg aacgcgctgg cggagcgttt
    23641 tggtggtgtg ctgctgtcag aaatttatga cgacgttagc ctggaagatg cgccgtactt
    23701 ctcagcactg tatggcccgt cacgccacgc catcgtggtg ccagatctgt cacaggtaac
    23761 cgaacacctg gaaggcttga ccgattgccc ggaagatctc tatttgatcg aaggggatcc
    23821 gcagtcattc gatgacagcg tgttcagcgt tgatgagctg gaaaaagcgg tggtggtgaa
    23881 aatcgccgat cgtcagtggc gttattcacg tttcccggaa gtgccgctgt ttggtcgtgc
    23941 tgcgcgtgaa agccgtattg aaagcctcca tgccgagcgg gaagtgcttt ccgaacgctt
    24001 cgccacgctc tcctttgatg tacagaaaac ccagcgtctg catcaggcgt tcagccgctt
    24061 tatcggcagt catctggcgg tggcgtttga gtctgacccg gaagcagaaa tccgtcaact
    24121 gaacagccgt cgcgtcgaac tggaacgggc gttgagtaat catgaaaatg ataaccagca
    24181 gcagcgtatt cagtttgagc aggcgaaaga gggcgttgcg gcgctgaacc gcattctgcc
    24241 gcgtctcaac ctgttggctg atgacagcct ggcggatcgc gtcgatgaaa tccgcgaacg
    24301 tctggatgaa gcccaggaag ccgcgcgttt tgttcagcag tttggcaatc aactggcgaa
    24361 actggaaccg atcgtttcgg tattgcagag cgacccggaa cagttcgaac agttaaaaga
    24421 agattacgcg tactctcagc agatgcagcg cgatgcccgt cagcaggcgt ttgccctgac
    24481 ggaagtggta cagcgtcgtg cgcactttag ctactctgac tcggcagaaa tgcttagcgg
    24541 taacagcgat ctcaacgaaa aactgcgtga acgtctggaa caggcggaag cggagcgtac
    24601 ccgcgctcgt gaagcgttgc gcggacacgc agcgcagttg aatcagtaca accaggtgct
    24661 ggcttcgctg aaaagttcgt atgacaccaa aaaagagcta cttaacgatc tgcaacgtga
    24721 attgcaggat atcggcgtgc gtgctgatag cggggcagaa gagcgggcac gtattcgccg
    24781 tgacgagttg cacgcgcaac tgagcaataa ccgttcacga cgcaatcaac tggaaaaagc
    24841 gcttaccttc tgcgaagcgg agatggacaa cctgacccgt aaactgcgca agctggagcg
    24901 ggattacttt gagatgcgcg agcaggtagt gaccgccaaa gcgggctggt gtgcggtgat
    24961 gcgcatggtg aaagataatg gcgttgagcg tcgcttacac cgtcgtgagc tggcttatct
    25021 ctccgctgat gatttgcgtt ccatgtcgga taaggcgtta ggtgcgctgc gtctggcggt
    25081 ggcagataac gaacatctgc gcgacgtgct gcggatgtcg gaagatccaa aacgtccgga
    25141 acgtaaaatt cagttcttcg tggcggttta tcagcatctg cgtgaacgta ttcgtcagga
    25201 tattattcgt accgatgatc cggtggaagc tatcgaacag atggagatcg agcttagccg
    25261 tctgaccgaa gaattaacct cccgtgaaca gaaactggcg atcagttccc gcagcgtggc
    25321 gaacatcatt cgcaaaacca ttcagcgcga gcagaaccgt atccgtatgc tcaaccaggg
    25381 gttgcagaac gtatcgtttg gccaggtgaa cagcgtacgt ctgaacgtga acgtacgtga
    25441 aacgcacgcc atgctgctgg atgtgctctc tgaacagcac gagcagcatc aggatctgtt
    25501 taacagcaac cgcctgacct tctcagaagc gctggcgaaa ctgtatcaac gtcttaaccc
    25561 gcagattgat atggggcagc gcacgccgca gaccatcggt gaagaactgc tggattaccg
    25621 caactatctg gaaatggaag ttgaggttaa ccgtggttcc gatggctggt tgcgtgcaga
    25681 gtctggtgca ttgtcgaccg gtgaggcgat tggtaccggt atgtcgattc tggtgatggt
    25741 ggtacaaagc tgggaagatg aatctcgccg cctgcgcggt aaagatatct ctccttgccg
    25801 cctgctgttc ctcgatgaag cagcgcgact ggacgcccgt tccatcgcca cgctgtttga
    25861 attgtgtgaa cgtttgcaaa tgcagctcat tatcgcagcg ccggaaaata tcagcccgga
    25921 gaaaggcacc acctataaac tggtgcgtaa agtcttccag aataccgaac acgttcacgt
    25981 cgtcggcctg cgaggatttg cgcctcaact ccctgaaacg cttccaggaa gtgacgaagc
    26041 gccttctcag gcgagttaaa gaaaaggcgg cagcaatgcc gccttttctt tttccgaaaa
    26101 ctccgtttct gcactaaaaa agtggcacat tacggcgcgc ttttctttaa acttctttac
    26161 attaggttat gtaaaaacgt atcggcgttt atatactgaa gataagtctg atgagtaaca
    26221 ggcttgctcg tcatactttc gtgagtattg gcgttgtaca ggcaagtcgt aaaataacag
    26281 cctggctatt cagagtatga taaaaacagg gggcaaggga tgttgcttaa tatgatgtgt
    26341 ggtcgtcggc tgtcggcaat cagtttgtgc ctggccgtaa cattcgctcc actgttcaat
    26401 gcgcaggccg atgagcctga agtaatccct ggcgacagcc cggtggctgt cagtgaacag
    26461 ggcgaggcac tgccgcaggc gcaagccacg gcaataatgg cggggatcca gccattgcct
    26521 gaaggtgcgg ctgaaaaagc ccgcacgcaa atcgaatctc aattacccgc aggttataag
    26581 ccggtttatc ttaaccagct tcaattgttg tatgccgcac gcgatatgca acccatgtgg
    26641 gaaaaccgtg atgctgttaa agccttccag caacagctgg cagaggtggc gattgccggt
    26701 ttccagccgc agtttaataa atgggtagag ttactgaccg atcctggtgt taacgggatg
    26761 acacgcgacg tggtgctctc tgatgcgatg atgggctatc tccatttcat tgcaaatatt
    26821 ccggtcaaag gcactcgctg gctatatagc agtaaacctt atgcgcttgc aacgccgccg
    26881 ctttcggtga ttaaccaatg gcagctggcg ctggataaag gtcaattgcc tacgtttgtt
    26941 gcaggactgg caccgcagca tccgcaatat gcggcgatgc atgaatcgtt actggcctta
    27001 ctcagtgaca ccaaaccgtg gccccaactg accggcaaag caacgttgcg cccaggacag
    27061 tggagtaacg acgtaccggc gttgcgcgaa atattgcaac gtacaggcat gttggacggg
    27121 ggaccgaaaa ttacgctacc tggcgatgac acgccaactg acgcggtagt cagcccatcc
    27181 gctgttacta ttgaaacagc agaaactaag ccgatggaca agcaaactac gtctcgtagt
    27241 aaacctgcgc ctgtcgttcg tgccgcctac gataatgaac tggtggaagc cgttaaacgt
    27301 tttcaggcat ggcaaggatt gggggcagat ggtgctattg gcccggcaac gcgtgactgg
    27361 ttaaacgtaa cgcccgccca gcgtgctggc gtgttggcac tcaacatcca gcgattgcgc
    27421 ttgctgccaa cagagctttc taccgggatc atggttaaca ttccggccta ttcgctggtc
    27481 tactatcaga acggcaatca ggtgctggat tcgcgagtca ttgtcggtcg ccccgatcgc
    27541 aaaacgccaa tgatgagcag tgcccttaac aacgtagtgg taaacccgcc gtggaacgta
    27601 ccaccaactc tggcacgcaa agatattctg ccgaaagtgc gcaacgatcc gggatatctc
    27661 gaaagccatg gctatacggt gatgcgcggc tggaacagca gagaagcgat tgacccatgg
    27721 caggttgact ggtctacaat cacggcctcg aatttaccgt tccgcttcca gcaggctcca
    27781 ggcccacgga actcgctggg gcgctataaa ttcaatatgc cgagttcaga ggccatttat
    27841 ttgcatgaca cgccgaacca caatctgttc aagcgtgata cacgcgcatt gagctcaggc
    27901 tgtgtacgag tgaataaagc ttccgatctg gcgaatatgc tgttgcagga tgcaggctgg
    27961 aatgacaaac gtatttctga tgcgctgaag cagggcgata cgcgttacgt caatattcgg
    28021 cagtcgattc cggtgaatct ctactatctg acggcctttg ttggtgcaga tggtcgtacc
    28081 cagtatcgta cagatattta caattatgat ctgcctgcgc gatccagctc gcaaattgta
    28141 tcgaaagcgg aacaattaat caggtaaatg aagcagttcg gggaaatcag ttgtcgtaat
    28201 aattagcatg atcgggggcg attctctgca gcccccgtca ctgctggggt tgagtcatct
    28261 tgacgtctgc tttacgggcg gttaaggtgc ctcttgtgcg ccagaagtgc atataaacga
    28321 taacattgac ctgtagactt gattatcatg gacaaattcg acgctaatcg ccgcaaactg
    28381 ctggcgcttg gtggcgttgc actcggtgcc gccatcctgc cgaccccggc gtttgcaaca
    28441 ctctctaccc cacgcccgcg cattttgaca ctcaataatc ttcataccgg agagtcaatc
    28501 aaagcggagt ttttcgatgg cagaggctat attcaggaag aattggcaaa acttaaccat
    28561 tttttccgcg attaccgcgc gaacaaaata aagtccatcg acccaggatt attcgaccag
    28621 ttgtatcgcc tgcaagggtt gttaggcacc cgcaaaccgg tgcaactcat ttccggttat
    28681 cgttccattg ataccaacaa tgaactacgc gcccgcagcc gtggagtagc gaagaaaagc
    28741 tatcacacta aaggccaggc gatggatttc cacattgaag gtatcgcgtt aagcaatatt
    28801 cgcaaagccg cgttatctat gcgcgcaggt ggtgtaggat attacccacg tagtaacttt
    28861 gtgcatattg ataccgggcc agcacggcac tggtagtaat cgcttaacga aacaggggca
    28921 gtatgaacta tcgtattatt ccggtcaccg cattctccca gaactgttca ttgatctggt
    28981 gtgaacaaac ccgtctggcc gcactggtcg atcctggcgg cgatgcggaa aaaatcaaac
    29041 aggaagttga tgccagcggc ctgacactga tgcagatcct gctgactcat ggtcatctgg
    29101 accacgttgg cgcagcggcg gaactggcgc aacattacgg cgtgccggtt tttggcccgg
    29161 aaaaagaaga tgagttctgg ctgcaaggct tgcctgcgca aagtcgtatg tttggtctgg
    29221 aagagtgcca gccgctgacg ccagatcgtt ggctgaacga aggcgatacc atcagcatag
    29281 ggaatgtgac tttacaggtg ttacattgcc ctgggcatac gccgggtcat gtcgtgtttt
    29341 ttgatgatcg ggcaaagctg ctgatttctg gcgacgttat tttcaaaggt ggagtagggc
    29401 gcagtgactt cccgcgtggc gatcataatc aactgatttc ttcaatcaaa gataaattgc
    29461 tgccactggg ggatgacgtg acatttattc cgggtcacgg accattatcc acacttggtt
    29521 atgagcgcct gcataatccc ttcctgcaag acgaaatgcc cgtctggtaa aggcgcataa
    29581 aaaagcccgc tttcaagcgg gcttcatcgg ttttaacgct tacagcactg ccacaatcgc
    29641 ttcgcacagc ggcgccatgt tatctggtgt catcccggcc acgtttacgc gaccagaagc
    29701 aacagcatat acgccaaact cttcgcgcag acgcagcact tgttcttttg tcaggccact
    29761 gaaggagaac atgccgttct gtttgatgat aaagctgaag tcgcgatttg cgcctttttc
    29821 ctgcagcgta ttgacgaaca actgacgcat acgctgaata cgctggcgca tatcagtcag
    29881 ttcttgttcc caaatcgcac gtaacgcatc gttgctcagg atggtggcaa caacagaagc
    29941 gccgtgtgct ggtgggttag agtagttagc acgaatcgcc gctttcattt ggctgaatgc
    30001 gcgttcaaca gtttcactgt cagcagcaac cagagtacaa gcgccaacac gctcgttgta
    30061 caggccaaag tttttagagt aggaactggc aacaatcagc tccttatgca gagccgcgaa
    30121 agcgcgcagt ccttcagcat cttcttccag accacgggca aaaccctggt aagcgaagtc
    30181 aaacagcggt aaccagcctt tctcaacgga gagttgtgcc agtgtctgcc attgttccag
    30241 cgtagggtcg atgccggttg ggttatggca gcagccatgg aacagcacta cgtcgccagc
    30301 ctgggcttcg ttcaggctgt taatcagtgc atcgaagtca agagtgtgat tttccgcatc
    30361 ataataagcg tattcacgaa cttccagacc tgcagagtta aagacgctct tatggttcgg
    30421 ccagcttggg ttgctcaccc acacacgctt aacgctggta ttttttgcca ggaaatcggc
    30481 agccacgcgt agtgcgccag tgccacccgg agtctgtgcc gtgcgagcac gtttgtcatt
    30541 gatcagggcg ctacctttac caaacagcag ttcctgagtg cagcgaccaa attcagggat
    30601 gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg agcagatact gttcagcctt
    30661 tttcacgctg gtcagtaccg gggttttacc cgtctcatct ttatagacac caatcccgag
    30721 gttaattttg ccgggacgtt catcggcacg aaacagatcg gccaggccca gaatcgggtc
    30781 ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc cattatggtt acagaaggga
    30841 agtccgctat cagggtaacg ggagatttac aaaattccaa ctattactga tgaaaacgca
    30901 ggctgttttt gcaagacgtg agattgctct ggaaggcata aaaaaaacag gaccaaagtc
    30961 ctgttttttc ggcatttaac aaagaggtgt gctattagaa ctggtaaacg atacccacag
    31021 caacggtgtc gtcagaacct acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt
    31081 agtcaacata ggtggacatg tttttgttga aatagtaggt tgcgcccact tcaaagtagt
    31141 tcaccagatc aacatcacca ataccttcta cgtctttcgc tttagatttg gtgtaagcga
    31201 tggacggacg cagaccgaaa tcaaactggt attgcgcaac taacagaacg tcttgcgttt
    31261 tgttggcgaa gccgctggtg tttgtaaatt tattagtgat cggcgtagcg ttacgggttt
    31321 caccgtagtt cgctgccagg tagatgttgt tcgcgtcata cttcaggcca gtagcccact
    31381 gttcagcttt tttgcctttg ccaagggaag actcttcctg caggttggta cggtcagcgg
    31441 caccataagc accaacgata ccaaagcctt cgtattcgta gctgatagaa ccgccaacac
    31501 cgtcgccgtt agagcggcgt gcagtgtcac gctcgttttt acccaggtac tgaacagcga
    31561 agttcagacc atcaaccaga ccaaagaagt tggagttacg ataggtagca acgccgccaa
    31621 cacgaccaac gaagaaatca tcgctgtatg cagtatcacc accaaattct ggcagcatat
    31681 cggtgtaacc cagtgcatca taaaccacac cgtagttacg gccgtaatcg aaagaaccaa
    31741 cgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt gttaccagtt tgagcgtcag
    31801 cgccttcaga gttgttaccc tggaagttat attcccactg accataaccg gtcagatcgg
    31861 aattgatttg agtttcccct ttaaaaccaa gacgggcata ggtcatgtcg ccattgccac
    31921 cgtaactgtt ttcaccgtta cccttggaga aataatgcag accgacagct ttaccgtaca
    31981 gatctacttt gttgccatct ttgttataga tttctgcagc gtttgcagta cctgctacta
    32041 acagagcagg gacgatcact gccagaatat tgcgcttcat cattatttat taccctcatg
    32101 gtttttttat gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac
    32161 tattgatgag agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt
    32221 ttcgctaccg tgaaagtgct acaaagataa agatttagtt tcaaaaagaa aaaatatgtg
    32281 acaaaaagta aaaattaagg aactttgtga acaccgtcat atttccatag aa
//
LOCUS       DAZFRE010000056        31949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFRE010000056 DAZFRE010000000
VERSION     DAZFRE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.056"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            105..1499
                     /gene="galP"
                     /locus_tag="ACHKUI_002898"
     CDS             105..1499
                     /gene="galP"
                     /locus_tag="ACHKUI_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6599062.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITSSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1576..2073
                     /gene="yggI"
                     /locus_tag="ACHKUI_002899"
     CDS             1576..2073
                     /gene="yggI"
                     /locus_tag="ACHKUI_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6599063.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2168..2875
                     /gene="endA"
                     /locus_tag="ACHKUI_002900"
     CDS             2168..2875
                     /gene="endA"
                     /locus_tag="ACHKUI_002900"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6599064.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2955..3686
                     /gene="rsmE"
                     /locus_tag="ACHKUI_002901"
     CDS             2955..3686
                     /gene="rsmE"
                     /locus_tag="ACHKUI_002901"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6599065.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     TEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3699..4649
                     /gene="gshB"
                     /locus_tag="ACHKUI_002902"
     CDS             3699..4649
                     /gene="gshB"
                     /locus_tag="ACHKUI_002902"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6599066.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTHTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4758..5321
                     /gene="yqgE"
                     /locus_tag="ACHKUI_002903"
     CDS             4758..5321
                     /gene="yqgE"
                     /locus_tag="ACHKUI_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6599067.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5321..5737
                     /gene="ruvX"
                     /locus_tag="ACHKUI_002904"
     CDS             5321..5737
                     /gene="ruvX"
                     /locus_tag="ACHKUI_002904"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6599068.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKSKVDSASAVIILESYFEQGY"
     gene            complement(5911..6891)
                     /gene="yggR"
                     /locus_tag="ACHKUI_002905"
     CDS             complement(5911..6891)
                     /gene="yggR"
                     /locus_tag="ACHKUI_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6599069.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6909..7613
                     /gene="yggS"
                     /locus_tag="ACHKUI_002906"
     CDS             6909..7613
                     /gene="yggS"
                     /locus_tag="ACHKUI_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6599070.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRYFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7631..8197
                     /gene="yggT"
                     /locus_tag="ACHKUI_002907"
     CDS             7631..8197
                     /gene="yggT"
                     /locus_tag="ACHKUI_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6599071.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFVKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8194..8484
                     /gene="yggU"
                     /locus_tag="ACHKUI_002908"
     CDS             8194..8484
                     /gene="yggU"
                     /locus_tag="ACHKUI_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6599072.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8492..9085
                     /gene="rdgB"
                     /locus_tag="ACHKUI_002909"
     CDS             8492..9085
                     /gene="rdgB"
                     /locus_tag="ACHKUI_002909"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6599073.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9078..10214
                     /gene="hemW"
                     /locus_tag="ACHKUI_002910"
     CDS             9078..10214
                     /gene="hemW"
                     /locus_tag="ACHKUI_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6599074.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLNNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EAVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10279..11286)
                     /gene="yggM"
                     /locus_tag="ACHKUI_002911"
     CDS             complement(10279..11286)
                     /gene="yggM"
                     /locus_tag="ACHKUI_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6599075.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQRTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11403..12449)
                     /gene="ansB"
                     /locus_tag="ACHKUI_002912"
     CDS             complement(11403..12449)
                     /gene="ansB"
                     /locus_tag="ACHKUI_002912"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6599076.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(12625..13344)
                     /gene="yggN"
                     /locus_tag="ACHKUI_002913"
     CDS             complement(12625..13344)
                     /gene="yggN"
                     /locus_tag="ACHKUI_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6599077.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(13528..13854)
                     /gene="yggL"
                     /locus_tag="ACHKUI_002914"
     CDS             complement(13528..13854)
                     /gene="yggL"
                     /locus_tag="ACHKUI_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6599078.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKMEEVRTSEL
                     FDVWWD"
     gene            complement(13854..14573)
                     /gene="trmB"
                     /locus_tag="ACHKUI_002915"
     CDS             complement(13854..14573)
                     /gene="trmB"
                     /locus_tag="ACHKUI_002915"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6599079.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            14734..15786
                     /gene="mutY"
                     /locus_tag="ACHKUI_002916"
     CDS             14734..15786
                     /gene="mutY"
                     /locus_tag="ACHKUI_002916"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6599080.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAVLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQISADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            15814..16089
                     /gene="yggX"
                     /locus_tag="ACHKUI_002917"
     CDS             15814..16089
                     /gene="yggX"
                     /locus_tag="ACHKUI_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6599081.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            16154..17233
                     /gene="mltC"
                     /locus_tag="ACHKUI_002918"
     CDS             16154..17233
                     /gene="mltC"
                     /locus_tag="ACHKUI_002918"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6599082.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            17435..18691
                     /gene="nupG"
                     /locus_tag="ACHKUI_002919"
     CDS             17435..18691
                     /gene="nupG"
                     /locus_tag="ACHKUI_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6599083.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(18740..20875)
                     /gene="speC"
                     /locus_tag="ACHKUI_002920"
     CDS             complement(18740..20875)
                     /gene="speC"
                     /locus_tag="ACHKUI_002920"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6599084.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            complement(21038..21115)
                     /locus_tag="ACHKUI_002921"
     CDS             complement(21038..21115)
                     /locus_tag="ACHKUI_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599085.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            21268..21975
                     /gene="yqgA"
                     /locus_tag="ACHKUI_002922"
     CDS             21268..21975
                     /gene="yqgA"
                     /locus_tag="ACHKUI_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6599086.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            22081..22156
                     /locus_tag="ACHKUI_002923"
     tRNA            22081..22156
                     /locus_tag="ACHKUI_002923"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22114..22116,aa:Phe,seq:gaa)
     gene            22333..23511
                     /locus_tag="ACHKUI_002924"
     CDS             22333..23511
                     /locus_tag="ACHKUI_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599087.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLSLGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLATPKAAHFPHLRADELPVFLRR
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSQQATDILLQLRTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSSWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSSQ
                     "
     gene            complement(23881..24126)
                     /locus_tag="ACHKUI_002925"
     CDS             complement(23881..24126)
                     /locus_tag="ACHKUI_002925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599088.1"
                     /translation="MNIHAQWCIIHRMDISLLTTTRDPEQLRTLAIAMVQKVKAENAG
                     LLDRMRILEEQMKLSLQQRFGKKCESLIGLRCQSVIE"
     gene            complement(24133..24513)
                     /gene="tnpB"
                     /locus_tag="ACHKUI_002926"
     CDS             complement(24133..24513)
                     /gene="tnpB"
                     /locus_tag="ACHKUI_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6599089.1"
                     /translation="MLTPDNVWLAVKPVDMRAGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKARSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTPDEFHWLVCGVDWPQV
                     KGHDLAKWVYREPPPDAQHITVTT"
     gene            complement(24500..24835)
                     /locus_tag="ACHKUI_002927"
     CDS             complement(24500..24835)
                     /locus_tag="ACHKUI_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6599090.1"
                     /translation="MPDTRWTLEQKRQHVGAWRASGLSRMAYCEQHNIPHKSMRYWPK
                     DIARADRKAHGPAFIPVSVSGLPESRGTTATAPADEPVSLYLPGGIRLCCRAAQLADV
                     FRALRHADT"
     gene            24936..25157
                     /locus_tag="ACHKUI_002928"
                     /pseudo
     CDS             24936..25157
                     /locus_tag="ACHKUI_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(25160..25492)
                     /locus_tag="ACHKUI_002929"
     CDS             complement(25160..25492)
                     /locus_tag="ACHKUI_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599091.1"
                     /translation="MFIHSQMVTSDSVICLGVYDDYQWFRKELKTQKQSRKEITNDSL
                     HRKHISEKETGRWIEGYKKATELASSCPDTQIISVSDREGDIYTSLNVQKMKVSEPPR
                     VSWRVFYL"
     gene            complement(26695..27177)
                     /locus_tag="ACHKUI_002930"
     CDS             complement(26695..27177)
                     /locus_tag="ACHKUI_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr family adhesin structural subunit"
                     /protein_id="HFO6599092.1"
                     /translation="MKKLAIMAATSTILAMSSSHAAFTGSGSTGTTKLTVTEQCQVLV
                     TGSDATKTRGELTDGARVGGLSVTAKGCNTEHAALRAQPDNYHQGKIVLLRDDQQARI
                     NVRLQSSDGSAWNTNGDTVYRADAGNWGGNLFVVVDGDNVDKPAGSYILNLDGGYWVS
                     "
     gene            complement(27379..27552)
                     /locus_tag="ACHKUI_002931"
     CDS             complement(27379..27552)
                     /locus_tag="ACHKUI_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DraP"
                     /protein_id="HFO6599093.1"
                     /translation="MGNLPVGSRGLYKGKKRSAGMNELRRPGSTQMTRTGQAHRGGEH
                     RLNAGPPDWQTVE"
     gene            complement(27924..28367)
                     /gene="draD"
                     /locus_tag="ACHKUI_002932"
     CDS             complement(27924..28367)
                     /gene="draD"
                     /locus_tag="ACHKUI_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial tip invasin DraD"
                     /protein_id="HFO6599094.1"
                     /translation="MNGSIRKMMRVTCGMLLMVMSGVSQAAELHLESRGGSGTQLRDG
                     AKVATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGGDGWSPVKG
                     EGGKGVSRPGQEEQVFFDVMADGNQDIAPGEYRFSVGGACVVPQE"
     gene            complement(28383..30965)
                     /gene="afaC"
                     /locus_tag="ACHKUI_002933"
     CDS             complement(28383..30965)
                     /gene="afaC"
                     /locus_tag="ACHKUI_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153002854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaC-I/III family usher protein"
                     /protein_id="HFO6599095.1"
                     /translation="MRDTSSGRMKTGVTGLALAVMVACVMFRAESGIARTYSFDAAML
                     KGGGKGVDLTLFEEGGQLPGIYPVDIILNGSRVDSQEMAFHAERDAEGRPYLKTCLTR
                     EMLARYGVRIEEYPALFRASGEGRGASVAEEACADLTAIPQATESYQFAAQQLVLGIP
                     QVALRPQLRGIAPEALWDDGIPAFLLNWQANAGRSEYRGYGKRVTDSYWVSLQPGINI
                     GPWRVRNLTTWNRSSGQSGKWESSYIRAERGLNGIKSRLTLGEDYTPSDIFDSVPFRG
                     AMMSSDESMVPYNLREFAPVVRGIARTQARIEVRQNGYLIQSQTVAPGAFALTDLPVT
                     GAGSDLQVTVLESDGTAQVFTVPFTTPAIALREGYLKYNVTAGQYRSSDDAVEHTSLG
                     QVTAMYGLPWGLTVYGGLQGAEHYQSAALGLGWSLGRLGAVSLDTTHSRGQQKGHDYE
                     TGDTWRIRYNKSFELKGTSFTAASYQYSSDGYHTLPDVLDTWRDDRYAYRHTENRSRR
                     TTLSLSQSLGQWGYVGLNGSRDEYRDRPHRDYFGASYSTSWNNISLSVNWSRNRNSGG
                     YYGGWSRTEDSVSMWMSVPLGRWFGGTDNDISATAQMQRSTGQDTRYEAGLNGRAFDR
                     RLYWDVREQMVPGSESHADTSRLNLTWYGTYGELTGMYSYSSTMRQLNAGMSGSMVAH
                     SEGVTFGQRTGDTVALIAAPGVSGASVGGWPGVRTDFRGYTLAGYASPYQENVLTLDP
                     TTFPEDAEVPQTDRRVVPTKGAVVRAGFRTRVGGRALVSLARQDGTPLPFGAVVTVEG
                     EAGQAAGSAGVVGDRGEVYLSGLKESGKLKAQWGENSLCHADYRLPEEKGPAGIFLTR
                     TVCM"
     gene            complement(31005..31802)
                     /gene="draB"
                     /locus_tag="ACHKUI_002934"
     CDS             complement(31005..31802)
                     /gene="draB"
                     /locus_tag="ACHKUI_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021517431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial biogenesis chaperone DraB"
                     /protein_id="HFO6599096.1"
                     /translation="MKMRAVAVFTGMLTGVLSVTGLLSAGAYAAGGEGNMSASATETN
                     ARVFSLHLGATRVVYNPASSGETLTVINDQDYPMLVQSEVLSEDQKSPAPFVVTPPLF
                     RLDGQQSSRLRIVRTGGEFPSDRESLQWICVKGIPPKEDDRWAEGKDGEKKADKVSLN
                     VQLSVSSCIKLFVRPPAVKGRPDDVAGKVEWQRAGNRLKGVNPTPFYINLSTLTVGGK
                     EVKEREYIAPFSSREYPLPAGASGKVQWKVITDYGGTSKQFEAELKG"
BASE COUNT         7391 a         8813 c         7870 g         7875 t
ORIGIN      
        1 atgttagtgt aagcgattac actgatgtga tttgcttcac atctttttac gtcgtactca
       61 cctatcttaa ttcacaataa aaaataacca tattggaggg catcatgcct gacgctaaaa
      121 aacaggggcg gtcaaacaag gcaatgacgt ttttcgtctg cttccttgcc gctctggcgg
      181 gattactctt tggcctggat atcggtgtaa ttgctggcgc actgccgttt attgcagatg
      241 aattccagat tacttcgcac acgcaagaat gggtcgtaag ctccatgatg ttcggtgcgg
      301 cagtcggtgc ggtgggcagc ggctggctct cctttaaact cgggcgcaaa aagagcctga
      361 tgatcggcgc aatcttgttt gttgccggtt cactgttctc tgctgctgcg ccaaacgttg
      421 aagtactgat tctttcccgc gttttgctgg ggctggcagt gggtgtggcc tcttataccg
      481 caccgctgta cctctctgaa attgcgccgg aaaaaattcg cggcagtatg atctcgatgt
      541 atcagttgat gatcactatc gggatcctcg gcgcttatct ttctgatacc gccttcagct
      601 acaccggtgc atggcgctgg atgctgggtg tgattatcat cccggcaatt ttgctgctga
      661 ttggtgtctt cttcctgcca gacagcccac gttggtttgc cgccaaacgc cgttttgttg
      721 atgccgaacg cgtgctgcta cgcctgcgtg acaccagcgc ggaagcgaaa cgcgaactgg
      781 atgaaatccg tgaaagtttg caggttaaac agagtggctg ggcgctgttt aaagagaata
      841 gcaacttccg ccgcgcggtg ttccttggcg tactattgca ggtaatgcag caattcaccg
      901 ggatgaacgt catcatgtat tacgcgccga aaatcttcga actggcgggt tataccaaca
      961 ccaccgagca aatgtggggg acagtgattg tcggcctgac caacgtactt gccaccttta
     1021 tcgcaatcgg ccttgttgac cgctggggac gtaaaccaac gctaacgctg ggcttcctgg
     1081 tgatggctgc tggtatgggc gtactcggta caatgatgca tatcggtatc cactctccgt
     1141 cggcgcagta tttcgccatc gccatgctgc tgatgtttat tgtcggtttt gccatgagtg
     1201 ccggtccgct gatttgggta ctgtgctccg aaattcagcc gctgaaaggc cgcgattttg
     1261 gcatcaccag ctccactgcc accaactgga ttgccaacat gatcgttggc gcaacgttcc
     1321 tgaccatgct caacacgctg ggtaacgcca acaccttctg ggtgtatgct gctctgaacg
     1381 tactgtttat cctgctgaca ttgtggctgg taccggaaac caaacacgtt tcgctggaac
     1441 atattgaacg taatctgatg aaaggtcgta aactgcgcga aatcggcgct cacgattaat
     1501 ctcctcaggc ttcctcccat catggaggaa gccacctctt gcagtcatct tttcttcgct
     1561 ctatcctctg ccgctatgaa aacatcccgt ctccctatcg ccatccaaca ggccgttatg
     1621 cgtcgcctgc gggaaaaact cgcccaggcc aacctgaagc tagggcgtaa ctacccggag
     1681 ccaaaactct cttacaccca gcgcggaacc tctgccggaa cagcctggct ggaaagctat
     1741 gaaattcgtc tcaatcccgt tttgctgttg gaaaacagtg aagcctttat tgaagaagtg
     1801 gtgccgcacg aactggcaca tttgctggta tggaaacatt tcggccgcgt agcgccacat
     1861 ggcaaagagt ggaagtggat gatggaaagc gtgctgggcg ttcccgcccg ccgtacgcat
     1921 cagttcgaac tgcaatccgt gcgccgcaac accttcccct accgctgcaa gtgccaggag
     1981 catcagctta ccgtacgccg tcataatcgc gtagttcgtg gcgaagccgt ctatcgctgt
     2041 gttcactgcg gcgaacagct ggttgcgaaa taaccacctg aactatcagg aactttcctg
     2101 atctggctga ttgcatacca aaacaacttt cgctacgttg ctggctcgtt ttaacacgga
     2161 gtaagtgatg taccgttatt tgtctattgc tgcggtggta ctgagcgcag cattttccgg
     2221 cccggcattg gccgaaggta tcaatagttt ttctcaagcg aaagccgcgg cggtaaaagt
     2281 ccacgctgac gcgcccggta cgttttattg cggatgtaaa attaactggc agggcaaaaa
     2341 aggcgttgtt gatctgcaat cgtgcggcta tcaggtgcga aaaaatgaaa accgtgccag
     2401 ccgcgtagag tgggaacacg tcgttcctgc ctggcaattc ggtcaccagc gccagtgctg
     2461 gcaggacggt ggacgtaaaa attgcgccaa agatccggtc tatcgcaaga tggaaagcga
     2521 tatgcataac ctgcagccgt cagtcggtga ggtgaatggc gatcgcggca actttatgta
     2581 cagccagtgg aatggcggtg aaggccagta cggtcaatgc gccatgaagg tcgatttcaa
     2641 agaaaaagtt gccgagccac cagcgcgtgc acgcggtgcc attgcgcgca cctacttcta
     2701 tatgcgtgac caatacaacc tgacactctc tcgtcagcaa acgcagctgt tcaacgcatg
     2761 ggacaagatg tatccagtta ccgactggga gtgcgagcgt gatgaacgca tcgcgaaggt
     2821 gcagggcaat cataacccgt atgtgcaacg cgcttgccag gcgcgaaaga gctaacctac
     2881 actagcggga ttctttttgt taacccctac cccacgcgta caaccgcgtg gggagacgac
     2941 gcggattttt aactatgcgt atcccccgca tttatcatcc tgaaccactg accagccatt
     3001 ctcacatcgc gctttgcgaa gatgccgcca accatatcgg gcgcgtactg cgtatggggc
     3061 cgggacaggc gctgcaattg tttgacggca gcaaccaggt ctttgacgcc gaaattacca
     3121 gcgccagcaa aaaaagcgtg gaagtgaagg tgctggaagg ccagatcgac gatcgcgaat
     3181 ctccgctgca tattcacctc ggtcaggtga tgtcgcgtgg tgaaaaaatg gaatttacta
     3241 tccagaaatc gatcgaactc ggtgtaagcc tcattacgcc acttttttct gagcgctgcg
     3301 gcgttaaact ggatagtgaa cgtctgaaca agaagctcca gcagtggcag aagattgcaa
     3361 ttgctgcctg tgagcagtgt ggccgtaacc gggtgccgga aatccgtcca gcgatggatc
     3421 tggaagcctg gtgtacagag caggatgaag gactgaaact gaatcttcac ccgcgcgcca
     3481 gtaacagcat caatacgttg ccgttaccgg tcgaacgcgt ccgcctgctg attggcccgg
     3541 aaggcggttt atcggcagat gaaattgcca tgactgcccg ctatcaattt actgatatcc
     3601 tgttgggacc tcgcgttttg cgtactgaga caactgcgct caccgccatt accgcgctac
     3661 aagtgcgttt tggcgatttg ggctaacgga gaagaataat gatcaagctc ggcatcgtga
     3721 tggaccccat cgcaaacatc aacatcaaga aagattccag tttcgctatg ttgctggaag
     3781 cacagcgtcg tggttacgaa cttcactata tggagatggc cgatctctat ttgatcaatg
     3841 gtgaggctcg cgctcgtacc catactttga gcgtcgaaca gaactacgat aaatggtacg
     3901 agttcacggg tgagcaggat ctgccgctgg ccgatctcga tgtgatcctg atgcgtaaag
     3961 acccgccgtt tgataccgag tttatctacg cgacctatat tctggaacgt gccgaagata
     4021 aagggacgct gatcgttaac aagccgcaga gcctgcgcga ttgtaacgaa aaactgttca
     4081 ctgcctggtt ctctgactta acaccagaaa cgctggtcac acgcaataaa gcgcagctaa
     4141 aagctttctg ggaaaaacac agcgacatca ttcttaagcc gctggacggt atgggcggcg
     4201 cgtcgatttt ccgcgtgaaa gaaggtgatc caaacctcgg cgtgattgcc gaaaccctga
     4261 ctgagcatgg cactcgctac tgcatggcgc aaaattacct gccagccatt aaagatggcg
     4321 acaaacgcgt gctggtagtg gatggcgagc cggtgccgta ttgcctggcg cgtattccgc
     4381 aggggggcga aacccgtggc aatctggctg ccggtggtcg cggtgaacct cgtccgctga
     4441 cggaaagtga ctggaaaatc gcccgtcaga ttgggccgac gctgaaagaa aaagggctga
     4501 tttttgttgg tctggatatc atcggcgacc gtctgactga aattaacgtc accagcccaa
     4561 cctgtattcg tgagattgaa gcagagtttc cggtgtcgat taccggaatg ttaatggatg
     4621 ccatcgaagc acgtttacag cagcagtaac ccaccttagc gagaaggatc tcgttgagac
     4681 tctgagtgac agcgcccttc tttccacgca tactgggcgc tgttgctttt ttgaaccagg
     4741 aaacagaacc tctgacaatg aatttacagc atcactttct tattgccatg cctgctctcc
     4801 aggatccgat tttccgtcgt tccgtggtct acatttgcga acataatacc aatggtgcaa
     4861 tggggatcat cgtcaacaag ccgctggaaa atctcaaaat tgaagggatt ctggaaaaac
     4921 tgaagatcac gccggagccg cgtgatgaat caatccgtct ggataaaccg gttatgctcg
     4981 gcggtccgct ggctgaagat cgcgggttta ttttgcatac tccgccctcc aattttgctt
     5041 ccagcattcg catttcagac aacacagtga tgaccacttc ccgcgatgtg ctggaaacgc
     5101 tcggcaccga taaacaaccg tctgacgtat tggtggctct gggttatgcc tcctgggaga
     5161 aaggtcaact ggaacaagaa attctcgata acgcgtggct aacggccccg gcagatctga
     5221 atattctgtt caaaacgccg attgcggacc gctggcgcga ggcggcaaaa ctgattggtg
     5281 tggatattct caccatgcct ggtgtggcag gacacgcctg atgagtggaa ccttactcgc
     5341 cttcgacttc ggcaccaaaa gcattggcgt agcggtcggt caacgcatta ccggcaccgc
     5401 tcgccctttg cctgcaatta aagcgcagga cggtacgccg gactggaaca ttatcgagcg
     5461 tttactgaaa gagtggcagc cggacgaaat catcgtcggt ttgccgctga atatggacgg
     5521 caccgaacag ccgttaaccg ccagagcgcg taaatttgcc aaccgtattc atggtcgttt
     5581 cggtgttgaa gtaaagctcc atgacgagcg tcttagcacc gtggaggcac gttccggtct
     5641 gtttgaacag ggcggctatc gggcgctcaa caaaagcaaa gtcgactctg cctctgcggt
     5701 tattattctc gaaagctatt tcgagcaggg atattaaggc gatttactag cgcacagatt
     5761 tctgacaatg tgctcgttgt ttcatgttgg atgcggcgta aacgccttat ccaacctaca
     5821 gtgagtgcga attcaaagtt acgcttccct tgtaggcccg ataagcttgc gcatcgggca
     5881 tatttacaat tcgtatcaga cacagcaacg tcacaagcgc ccttctttca cccgctgctg
     5941 ataactctgc tgaaacgtta tcatccccac ctgctgcccg gtttgaataa catgcggtaa
     6001 ctggtgggtt ttcccttcac gaatcaaatt ccccaccgcg ggtgtgttaa tcagtaattc
     6061 aaacagcgcc acgcgtcctt cctgtttatc cacttccagt ttttgtgaca acactgcccg
     6121 taaactacct gccagttgat tacgcacggg atctttttcc tgcgcaggaa atgaatccac
     6181 cagtcgctca actgcctgcg ctgcgccgcg cgtgtgtaat gtcgccagca ccagatgccc
     6241 ggtttctgcc gccgtcagcg ccagacggat tgtctcgcta tcacgcagct ctccgagcaa
     6301 aatcacatca ggatcttccc gcaatgcagc ccgcaatccc gatgcgaaag tcatacagtg
     6361 caaaccaatc tcccgctgct ggatcaaaca tcgctgactg gtatagagat attccacagg
     6421 atcttccagc gtcagaatat gcgcatcggc atgttgattg agatagccaa ccatcgccgc
     6481 cagcgtggta gatttgccgc tccccgtcgc ccccgtcacc agaatcaggc cattctcgct
     6541 cttgagtaat tccggcaata ccggtggtgc gccaagctgt tcgagctgcg ggcagtgcga
     6601 aggtaacagc cgtaacgcca gagaaatacc ttgccgttgt gcgaacgcac tgccgcgcaa
     6661 tcgctggttt tccgccagcg acacagcaaa atccagctga ccattctcca gcaatattgc
     6721 ccgctgatcg tcatccagcc actcccgcag tagctcttcg acgtccggcg cgtcaaacgg
     6781 cgcagcttcc attctcccgc gaatacgcca tcgtgcgggc caggcgctgc acaggtgtag
     6841 atccgagacg ttatgcttta cactaagggc cacaatttct tccatattca tactaagatc
     6901 ctcggaaaat gaacgatatt gcgcataacc tggcacaggt ccgggacaaa atctcagcgg
     6961 ctgcaacgcg ttgcggccgt tctccagaag aaattacgct gcttgcagtc agtaaaacaa
     7021 aacctgcgag cgccatcgca gaagccattg atgccgggca gcgtcaattt ggtgaaaact
     7081 acgttcagga aggggtagat aaaattcgct actttcagga actgggcgta acaggattag
     7141 aatggcattt tattggcccg ttgcagtcta ataaaagccg cctggtggca gagcatttcg
     7201 actggtgtca taccatcgac cgtttgcgca tcgctacccg tctgaacgac cagcgcccgg
     7261 cagaacttcc ccctcttaac gtactgattc aaattaacat tagtgatgaa aacagtaagt
     7321 ccgggattca actggctgaa ctggacgagc tggcagctgc ggtcgctgaa ctaccgcgtt
     7381 tacgtctgcg cgggctgatg gcaatccctg cgcctgagtc agaatatgta aggcagtttg
     7441 aagttgcacg ccaaatggct gtagcatttg ccggactgaa aacgcgctac ccgcatatcg
     7501 acacgctctc tctgggaatg tcggacgata tggaagccgc cattgcggca ggtagcacga
     7561 tggttcgtat cggcactgca atttttggtg cgcgtgatta ctctaaaaaa taaggaatta
     7621 aaggaacgcc atgaatacgt tgactttcct gctttcaacg gtcattgagc tgtataccat
     7681 ggtgctgtta ttacgcatct ggatgcagtg ggctcattgt gatttttaca accccttctc
     7741 acagtttgta gtgaaggtaa cgcagccgat tatcgggcca ctgcgccgcg ttattccggc
     7801 aatgggcccc attgatagcg cctcgctgct ggttgcctat attctcagtt tcgttaaagc
     7861 catcgtgctg tttaaagtgg tgaccttcct gccaatcatt tggattgccg gtttattgat
     7921 tctgctgaaa accatcggcc tgctgatatt ctgggtcctg ctggtgatgg cgattatgag
     7981 ctgggtaagc cagggtcgta gcccgattga atacgtgctg attcagctgg ccgatccgct
     8041 gctgcgcccg attcgccgct tgctaccggc aatgggtggg attgatttct cgccgatgat
     8101 cctcgttctg ctgctgtatg tcatcaatat gggtgtcgca gaagtcttgc aggcgaccgg
     8161 aaatatgctg ctgccggggc tgtggatggc gttatgagtg ccgtaacagt taatgatgac
     8221 ggtctggttt tacggctcta tattcagccg aaagccagcc gtgattctat tgtcggttta
     8281 catggcgacg aggtaaaagt cgccattacc gcgccgccgg ttgacggcca ggccaacagc
     8341 catctggtga agtttctcgg taagcaattc cgggttgcca aaagccaggt ggtgattgaa
     8401 aaaggcgaac ttggccgcca caaacaaatt aaaatcatta atccgcaaca aatcccgcca
     8461 gaaatcgcgg cgttaattaa ttaggtatcc tatgcaaaaa gttgtcctcg caaccggcaa
     8521 tgccggcaaa gtgcgtgagc tggcgtcgct gcttagcgac ttcggtcttg atatcgttgc
     8581 ccaaacagac ctcggcgtag attccgctga agaaaccggc ctgaccttta tcgaaaacgc
     8641 gattctgaaa gcgcgccatg cggcaaaagt gaccggttta ccggcaattg ccgacgactc
     8701 cggtctggcg gtagatgcgc tgggtggcgc gccgggtatt tactccgcgc gttattccgg
     8761 tgaagacgcg accgatcaaa agaatctgca aaaactgctg gaaacaatga aagacgtacc
     8821 ggacgaccaa cgtcaggcgc gtttccactg cgtgctggta tatctgcgtc acgcggaaga
     8881 tcccactccg ctggtgtgcc acggtagctg gccgggcgtg attactcgtg aaccagcggg
     8941 cactggtggc tttggttatg atccaatctt cttcgtacct tccgaaggga aaactgctgc
     9001 cgaactgacc cgcgaagaaa agagcgccat ttcccaccgt ggtcaggcgt tgaaactgct
     9061 gctggacgct ttacgtaatg gttaaattac cgccgctgag tctctacatt cacatcccgt
     9121 ggtgcgtgca gaaatgcccg tactgcgatt tcaactctca cgcgttgaaa ggagaagtgc
     9181 cgcacgacga ttatgttcag catctgctta acgatctgaa caacgatgtg gcttacgctc
     9241 agggccgtga agtaaagaca atctttattg gcggtggtac gccgagcctg ctttccggcc
     9301 cggcgatgca aacgctgctg gacggcgtac gtgcgcgttt gccgctggca gcggatgcag
     9361 aaattacgat ggaagcgaat cctggtacgg ttgaagccga tcgctttgtt gattatcagc
     9421 gtgctggtgt gaaccgcatc tccattggcg tacagagttt tagcgaagaa aagctgaaac
     9481 gacttgggcg cattcatggc ccgcaagaag cgaaacgagc tgcgaatctg gcaagcgggc
     9541 tggggctgcg tagttttaac cttgatttga tgcatgggct gccggatcaa tcactggaag
     9601 aggcgcttgg cgatctgcgc caggccattg aactgaatcc gccgcatctt tcctggtatc
     9661 aactgaccat cgaacctaat acgctgtttg gctcgcgccc tcctgtactg ccggacgatg
     9721 acgcgctgtg ggatattttc gaacaggggc atcagttatt aaccgcagcg ggttatcagc
     9781 aatatgaaac ttccgcttac gccaaacccg gttatcagtg ccagcacaat ctcaactact
     9841 ggcgctttgg tgactacatc ggtattggct gcggcgcgca tggcaaagtg accttcccgg
     9901 atgggcgcat tctgcgtacc accaaaacgc gtcatccacg tggttttatg caggggcggt
     9961 atctggaaag ccagcgagat gtcgaagccg cagataaacc atttgagttc tttatgaatc
    10021 gcttccgtct gctggaacct gcgccgcgcg tggagtttag ccagtataca ggcctttctg
    10081 aagcagttat tcgttctcag ttagacgagg ctatcgctca gggttatctc acagaatgtg
    10141 cggattactg gcagataacg gaacatggga agttgttttt aaattcgctg ctggagcttt
    10201 ttctggctga gtaaatttgt attgccggat gcggcgtgta cgcaacatcc ggctacaact
    10261 cttgcgtcat cgcaaaaatt acttaatcac cccattgcgc cgcagttgtt cggtatcttt
    10321 tttccagcgt tcattagcct ggttgatatc gtcaattttc atcaaaatcg cgactttttt
    10381 actgtttatt tctgaaacct tcatcgttaa tggatattgc cctttctcga gcgttatcca
    10441 gtcatcctgc aatttacggc tgagtttttg tgattgtaga aaagtctgtc gccgttgttc
    10501 gttgatatca aaatctcgtt tctgctgaat tggcacttca taacactctt cactctgttt
    10561 atgacatgcc gcaatcgccg gttgataaac ctctttgtca tatttaaccg cgaacgcctc
    10621 gctatcgttc tgtttattaa aatcatcacg ctgttggtga atttttttaa aggcgtcttc
    10681 gcgggtcctt tgcttaccgt ttgcatcttt tccccaatat gcatccgctg ctttctgcgc
    10741 cgccaccatt tgcttatcaa gcgcttttac atcctttttc agtgcaggaa tcgagttatt
    10801 ctggttattc aacgcagact caagctgact aaagcgataa ttgaaatctt cgctattcac
    10861 gatcagatat ttattattcg ttttaatatc gtcaaccacc cgcccacgat cgctggctgc
    10921 cgcctgaaaa gagtaaaagt tcaccgacca gccagacgcg ggcgttcctt tactggttaa
    10981 catcgccgag aatttcaccg gtttatcttt cgtccaggtc tgttcgagca gctcgtaatc
    11041 tgccaactga ccgacccagg tatagagatc gtcactggaa gagacatcgc cttccgctga
    11101 ccaggtggcc tgattaccct tagcatcaag attttttaag gtgatgacat caagcttgag
    11161 aatgccgtga tactgctttt tgaattgatc ttttaagata ttttcagttg gcggttcacc
    11221 atctgcccgg acattaccag tcatcaacat aagcagcgcc gtaccaacaa tccattgctt
    11281 tttcacccga aaaccctcgc gcggaataat ttattccggt gattatcatt agagtaataa
    11341 aaattggcaa tggacgtgag tctgaagtga ataagccccg gcacgatacc ggggcgaagt
    11401 gattagtact gattgaagat ctgctggatc tgctgcggat ctttagtttg cgtcagagcc
    11461 agttgcagca gaacgcgcgc tttttgcggg ttcaacgtgc cagaggcgac gaagccgtat
    11521 ttcgcatcat ccacttcggc atcctgagtg gtagcgcccg tcggtacgcg ggaagaacgc
    11581 actactgcag tgccgttttt cgcagcggtt gcaagggtgt caaatacggt tttatacagg
    11641 ttgccgttac ccacgccagc gctaacgatg ccatcatagc ccgcatctac cagtgcttta
    11701 gccggaagat cggatgcgtt agcgtagtta taaacaatgc cgactttcgg cagttcattc
    11761 agcttagaga catcgaacgg cgtgtcgctg gtgtgcttac gtgccggggt acgttggtag
    11821 tcaatcttac cgttgtgaat gtaacccaga ggaccgtagt taacagactt gaaggtcgct
    11881 acgtcggtgg tgttggtttt ggtgacatca cggccatcaa gtacggtgtc gttcatcact
    11941 accagtacgc cacgattagc ggaggcttta tcagctgcgg tcactaccgc gttatacagg
    12001 ttgaatggac cgtctgcgct catagacgtg gacgggcgca ttgcaccgac catcaccacc
    12061 ggtttgtcgc atttcaccgt caggtcgagg aagtaagcgg tttcttccat cgtgtcggta
    12121 ccgtgggtaa tgacgaagcc gtcagtttta tcgcagtcgg tgttaatttt tttcgccagt
    12181 gtcagccaga catcatcgtt catgtcctgg gagccaatat tcactacctg ctcgccttta
    12241 acgttcgcaa tgtccttcag ttgcggcacc gcattaacca gattttctac gccaacttta
    12301 cccgctgtgt agttagattt ggttgcggag tcaccaccac cggcaatggt cccgccggtt
    12361 gctaaaatgg tgatattggg taatgccaat gctgcaccac taaaacccat aaccagtgcg
    12421 gcaagtgccg tctttttgaa aaactccatt tcattcctcc agttacgtga acgctacgca
    12481 ttatccctta gctttgcatg ggaaatttga cgttaaacaa tttacaacgt gaatatattt
    12541 tggagatcta caaagttaga ggcaggtaac aaaacgaaga attaaacggc ataaaaaagt
    12601 attatgccgt cttaaaatag aggattattt taaattcccg accagggctt tgcggctatc
    12661 ttccagagtc acaacgcggc tacaaacatc tttgccaaac tgctggaaat ctttttcctg
    12721 ctttttccac tcggtttgga ttgaggattg cagaccgccc aggcttccca gcacgttctg
    12781 taatgggtta ccgccgcttt tcagcaccgc tttcgcgccc atttcattaa tgctgtcctg
    12841 taaaattccg cccattgcct gattcactaa ttgctggcct tcggcgcgaa cctgatcaat
    12901 ggctttatag tggaacgtca ggccatcgct gcgcgtttcg ataatgcggt tcatctgctc
    12961 tttcagctgc gcatcaagtt tggtcagacg gctgcgcatt ttgctgcttt cgcccatctc
    13021 ctgaacgata attttatcca gcgcaatacg ggctttttcg acgcggcttt tcgcgccttc
    13081 atcaatccac ggcagggtgc tacggagttc agcctgataa tccttcgcct gctcgcgctg
    13141 ggcggcattc agggaatatt gcttaccgtt atacatcacg ttgccgtctg gcgtgatcac
    13201 cagattgccg ttttcgccct tcacctgcac ggtttgcggg ctgacaatca catcgtcacg
    13261 cggcgtgacg ctgcactggt agtcggcgtg agcggtcatt gccgtcactg aaagtgctgc
    13321 cgccagcagc attttgcgca tcatagtctt ccctcaagaa caaatcaggc cagcatttgc
    13381 tggccccaga ttgataacaa agtgcgcgtt ttccatgccg gatgcggcgt aaacgcctta
    13441 tccggcctac tcgatcgtgc atctttgtag gcctgataag cgtagcacat caggcagttt
    13501 tgcatttgtc atcgcccgta tgctttctta gtcccaccaa acgtcgaaaa gttcgctggt
    13561 gcgtacctct tccattttgc gttcttccag ccacttacgc acaatcgcct gatgttcttc
    13621 ggtgcatttg ccgatttcct gcatgcagat cagaccttcc caggccagat aaccgctgcc
    13681 gtcaaaggcc agtttgttcg gttcgataac ctcgttaata aagtcatcaa cggttttatc
    13741 aatctgttct tccgatgtac cttccgggaa tcgccatgcc accgaaaatc ctaattcctg
    13801 gaattcgtcg atgtgcattt ttttacgcag acgacggcta cggttctttg ccattatttc
    13861 accctctcga acattaagtc ccatactccg tgaccaagac gatgaccacg ttgttcaaat
    13921 ttcgtcaccg gacgtgatgc cggacgcggt acgtaatcat tgctctctga caggttttta
    13981 taaccgtcaa tagaagacat cacttcaagc atatgttccg cataaggttc ccagtcggtc
    14041 gccatatgga atatgccccc cagctgcagt ttgcttttta ccagctcggc aaacggcacc
    14101 tgaacgatac ggcgtttatt atggcgcgct ttgtgccacg ggtcagggaa aaagagctgc
    14161 accatgcgca atgaattgtc aggaatcatt ttatgcagca cttcaaccgc atcgtgacac
    14221 atcacgcgca ggttgcttaa gccctcttca tgcgcagaag acaggcacgc accaacgccc
    14281 ggtgaatgca cttcaatgcc gaggaagtcc tgctcggggc gatctttagc cattgccacc
    14341 aacgacgcgc ccatgccaaa accaatctca agcgtcaccg gcgcttcacg gccaaaaagc
    14401 gcagggaaat ccagcatatc ttcgctgaac tcaacgccca tcaccggcca gtagttttcc
    14461 agcgcatgtt cctggccttt ggtcagtcgc ccctggcggc gcacaaaact acggatacgg
    14521 cgcagcgggc ggccgttttc atcaaattcc ggtgagatga cgtcgttttt cataaagatt
    14581 tagtcgcttg tgaaagtgtt ctgaaaacgg gcattatcca aagttagttg ccggatgcaa
    14641 gcatgataag gccgtggctg cggaaagttc cggtttacac cctgccgtcg ctgtgctgca
    14701 atcttgcccc caacaacagt gaattcggtg accatgcaag cgtcgcaatt ttcagcccag
    14761 gttctggact ggtacgataa atacgggcgg aaaacgctgc cctggcaaat tgacaagacg
    14821 ccctacaaag tatggctctc agaagtgatg ttgcaacaaa ctcaggttgc gaccgttatc
    14881 ccctattttg aacgctttat ggtgcgcttc ccaacggtga ccgatctcgc caatgcgccg
    14941 ctcgacgaag ttctccactt gtggaccggg cttggctatt acgcccgagc gcgcaatctg
    15001 cataaagcgg cacaacaagt ggcggcctta cacggcggta aattcccgga aacctttgag
    15061 gaagttgcag tactgccggg cgtcgggcgt tccaccgcag gcgcgattct ctcgctttct
    15121 ctgggtaagc actttccgat tctcgacggt aacgtcaaac gcgtgctggc gcgctgctat
    15181 gctgtaagcg gctggcccgg gaaaaaagag gtcgagaata aactatggag tttgagcgag
    15241 caggtgacgc ccgcggttgg cgtggaacgg tttaaccagg cgatgatgga tttgggcgcg
    15301 atgatttgta cgcgctctaa gccgaaatgt tcgctctgtc cgctacaaaa cggatgtatt
    15361 gccgccgcca acaatagctg gtcgctttat ccgggcaaaa aaccgaaaca gacgctgcca
    15421 gagcgcaccg gctacttttt gctgttacag cacgaagatg aagtattgct ggcgcagcgt
    15481 ccgccgagcg gattgtgggg cggtttatac tgtttcccgc agtttgccga tgaagaaagt
    15541 ttgcggcagt ggctggcgca acgacagatt tctgccgata acctgacgca gctgaccgcg
    15601 tttcggcata ccttcagcca tttccactta gatattgtgc ctatgtggct tcccgtgtcg
    15661 tcattcaccg gctgcatgga tgaagacaat gcgctctggt ataacttagc gcaaccgccg
    15721 tcagttggcc tggcggctcc cgtggagcgt ttgttacagc agttacgcac tggcgcgccg
    15781 gtttagcgcg tgagtcgata aagaggatga tttatgagca gaacgatttt ttgtactttc
    15841 ctgcaacgtg aagcagaagg tcaggatttt cagctgtacc ccggcgaact gggaaaacgc
    15901 atctataacg agatctccaa agaagcctgg gcgcagtggc agcacaaaca aaccatgctg
    15961 attaatgaaa agaaactcaa catgatgaat gccgagcacc gcaagctgct ggagcaggag
    16021 atggtcaact tcctgttcga gggtaaagag gtgcatatcg agggctatac gccggaagat
    16081 aaaaaataaa aacagtgccg gagcacgcct ccggcaactt gcataaaaac aaacacaaca
    16141 cgcacccgga atgatgaaaa aatatctcgc gctggctttg attgcgccgt tgctcatctc
    16201 ctgttcgacg accaaaaaag gcgataccta taacgaagcc tgggtcaaag ataccaacgg
    16261 ttttgatatt ctgatggggc aatttgccca caatattgag aacatctggg gcttcaaaga
    16321 ggtggtgatc gccggtccta aggactacgt gaaatacacc gatcaatatc agacccgcag
    16381 ccacatcaac ttcgatgacg gtacgattac tatcgaaacc atcgccggga cagaacctgc
    16441 cgcgcatttg cgccgggcaa taatcaaaac gttgctgatg ggggacgatc cgagttcggt
    16501 cgatctctat tccgacgttg atgatattac gatttcgaaa gaacctttcc tttacggtca
    16561 ggtggtggac aacaccgggc agccgattcg ctgggaaggt cgcgcgagca acttcgcgga
    16621 ttatctgctg aaaaaccgtc tgaagagccg cagcaacggg ctgcgtatca tctacagcgt
    16681 caccattaac atggtgccga accaccttga taaacgtgcg cacaaatatc tcggtatggt
    16741 ccgccaggcg tcacgcaaat atggcgttga tgagtcgctg attctggcaa ttatgcagac
    16801 tgaatcttcc tttaacccgt atgcggtcag ccgttccgat gcgctgggat taatgcaggt
    16861 ggtacaacat accgccggga aagatgtgtt ccgctcgcag gggaaatccg gcacgccgag
    16921 ccgcagtttc ttgtttgatc ctgccagcaa tattgatacc ggcaccgcgt atctggcgat
    16981 gctgaacaac gtttatctcg gcggaattga taacccaaca tcgcggcgtt atgccgtcat
    17041 caccgcctat aacggcggcg caggcagcgt gctacgagtc ttttcgaatg ataagattca
    17101 ggctgccaat attattaaca ccatgacgcc gggcgatgtt tatcagacgc tgacgacccg
    17161 ccatccctct gcggaatctc gccgttatct ttataaagtg aataccgcgc aaaaatctta
    17221 ccgccgccga taattccact aaccgcccct gacgatgctc aggggtaaaa atgttatcca
    17281 catcacaatt tcgttttgca aattgggaat gtttgcaatt atttgccaca ggtaacaaaa
    17341 aaccagtccg cgaagttgat agaatcgcat catctcgcac ggtcaaatgt gctttttcaa
    17401 acactcatcc gcatcacgat gtgaggaaat taacatgaat cttaagctgc agctgaaaat
    17461 cctctctttt ctgcagttct gtctgtgggg aagttggctg acgaccctcg gctcctatat
    17521 gtttgttacc ctgaagtttg acggtgcttc tatcggcgca gtttatagct cactgggtat
    17581 cgccgcggtc tttatgcctg cgctgctggg gattgtggcc gacaaatggt taagtgcgaa
    17641 atgggtctat gccatttgcc acaccattgg cgctatcacg ctgttcatgg cggcagaggt
    17701 cactacgccg gaggcgatgt tccttgtgat attgattaac tcgtttgctt atatgccaac
    17761 gcttgggtta atcaacacca tctcttacta tcgcctgcaa aatgccggga tggatatcgt
    17821 tactgacttc ccgccaatcc gtatctgggg caccatcggc tttatcatgg caatgtgggt
    17881 ggtgagcctg tccggcttcg aattaagcca catgcagctg tatattggcg cagctctttc
    17941 cgccgttctg gttctgttta ccctgactct gccgcatatt ccggttgcta aacagcaagc
    18001 gaatcagagc tggacaaccc tgctgggcct cgatgcattc gcgctgttta aaaacaagcg
    18061 tatggcaatc ttcttcatct tctcaatgct gctgggcgcg gaactgcaga ttaccaacat
    18121 gttcggtaat accttcctgc acagcttcga caaagatccg atgtttgcca gcagcttcat
    18181 cgtgcagcat gcgtcaatca tcatgtcgat ttcgcagatc tctgaaaccc tgttcattct
    18241 gaccatcccg ttcttcttaa gccgctacgg tattaagaac gtaatgatga tcagtattgt
    18301 agcgtggatc ctgcgttttg ctctgtttgc ttacggcgac ccgactccgt tcggtactgt
    18361 actgctggta ctgtcgatga tcgtttacgg ttgcgcattc gacttcttca acatctctgg
    18421 ttcggtgttt gtcgaaaaag aagttagtcc ggcaattcgc gccagcgcgc aggggatgtt
    18481 cctgatgatg accaacggct tcggctgtat cctcggcggc atcgtgagcg gtaaagtggt
    18541 tgagatgtac acccaaaacg gcattaccga ctggcagacc gtatggctga ttttcgcagg
    18601 ttactccgtg gttctggcct tcgcgttcat ggcgatgttc aaatataaac acgttcgtgt
    18661 cccgacaggt acacagacgg ttagccacta attacgcaaa gaaaaacggg tcgccagaag
    18721 gtgacccgtt tttttattct tacttcaaca cataaccgta caaccgtttc atgccattcg
    18781 catcggtttc gctatagaca ccttgcaact ccggcgaaaa tcccggcaac aaattcacgc
    18841 cttcttccag cgcaaggaaa taacgttgaa ccgccccacc ccagacttcc ccgggtacca
    18901 cgcaaagcac gccaggtgga taaggcaacg ccccttctgc cgcaattcgc ccttcggcat
    18961 cacgaatccg caccaactcc acttcaccgc gaatataagc gctatgcgca tcctggggat
    19021 tcatcaccac tgacgggaaa ctctgctggc ggaacatcgc tttttgtagg tctttgacgt
    19081 cgaaactgac atacagatcg tgcatctcct gacacaactg acgcagggtg tagtcgcgat
    19141 agcgcaccgg atatttgtta taaacgctcg gcaacacctc agccagcggc gagtcatcct
    19201 caatatgctg ttcaaactgc gccagcatcg ccaccagttg tgccagcttc tcgtggcttt
    19261 ccgccggagt taataagaac agaatggagt tgagatcgca cttctccggc acaatgccgt
    19321 tctcacgcag gtagtgcgcc agaatcgtcg ctggaacgcc aaaatcgcta tattcgccgg
    19381 tttcggcatc gatacccggc gtggtgagta acagcttgca cggatcaaca aaatactgat
    19441 ccgcggcata tccttcaaag ccgtgccact tcgcccccgg ctcaaaactg aaaaaacggc
    19501 ggtcgctggc taacactgac gtcggataat cctgccacaa tttgccatca acaacgggcg
    19561 ggataaacgg gcggaacagc ttacagcgcg caagaatcgc cttccgggct tcaatcccca
    19621 acgcaacgca ctccgcccac agccgacgcc cactctcccc ttcatgaatt ttggcgttaa
    19681 cgtccagcgc ggcgaacagc ggatagaacg ggctggtaga agcatggagc ataaaggcat
    19741 tattcaaccg cttatgcggg caaaaacgcg cctgtccacg gatatggtta tcttttttat
    19801 ggatctgcga cgtctgtgag aatcccgcct gctgtttgtg caccgactgg gtcacaaaga
    19861 tccccggatc gttttcgtta agttctaaca gcagcggcga gctatccgcc atcatcggga
    19921 taaactgttc gtaaccgacc cacgcggaat caaacagaat gtaatcacac agatgcccaa
    19981 cggtatcgat cacctgacgg gcgttataga cagtgccgtc ataggttccc agctgaataa
    20041 tcgccaggcg aaacgggcgc ggcaggtcgg ctttttctgg cgcaacgtcg cgaatttgct
    20101 ggcgcagata ctcttcatta aaacagtgcg catcaatacc gccaatgaaa ccaaacgggt
    20161 tacgtgaagc ttccagatag accggcgtcg cccccgcctg aatcaacgcg ccgtgatgat
    20221 tcgacttatg gttgttacgg tcgaagagca ccaaatcgcc acgcgttaac agcgcattcg
    20281 tcaccacttt attcgctgcc gatgtgccgt tcagcacaaa ataggtttta tcggcatgaa
    20341 agactttggc tgcgaatttc tgcgcatctt tcgccgatcc ttcatgaatc agcagatcgc
    20401 ccaattttac gtcagcgtta cacatatcgg cgcgaaagat gttctcgcca aagaaatcgt
    20461 aaaaatggcg tccggcagga tgctttttaa agaacgcacc atgttgatgt ccagggcaag
    20521 caaaggtgct attacccatc tcaacgtact gcgtcagcgt gtcataaaac ggtggcagca
    20581 aattctcttc atactgacag gctgcggatt ccagctccag ccactgctgc tcgttgccgt
    20641 tgattaccgc cgtaacgccc gcaggtaatt caacaacatg ttcggaatac aaaaacaccg
    20701 gtagatgaaa accggtgcgc ttaagcaacg caagaatgcc actgcgacta tccgcagcgg
    20761 taatgacgac tgccgcgacg tccgtaaaat cagtatctgc caacgccacc acgcggcgat
    20821 gagtagaaag tcgggatacc agttcactac tggcggcaat attcattgat ttcataagcg
    20881 caaaacccgt ttcggggaag taagaatacc ggacaaggtg aaaaaccctg ccccacgaga
    20941 tatgggtcaa actggtcacc agctccgacc gccagacatc agtaaaagca gaaacgctct
    21001 gattttactg ttgtcctgca gtgagcgtgc gttaacttca ccgcatgagc agaaaaaggg
    21061 aggagtttcg cgcgaaacgg cgataagcga gagaatgtaa ggaggtggcg tgcatcgggc
    21121 aaactccgta cagagaggag aaaattcgcg caatcatggc acctttcgct aaagcgtgca
    21181 agccaggact ttgcgaccag acaagccatc aacaaggcaa aaagttcata ataagaaaat
    21241 caaacaatac agttaacagg aactcttgtg gtcatcggcc cttttatcaa cgctagtgca
    21301 gtcttactgg gtggcgttct cggcgcactg ctcagccaac gcttaccgga acgtatccgc
    21361 gtctccatga catcaatttt tggtctggca tcgctgggga ttggtatttt actggtggtg
    21421 aaatgcgcca accttccggc gatggtttta gcaaccttac tcggtgcatt aatcggcgaa
    21481 atttgtctgc tggaaaaagg cgtcaatacc gcggtcacca aagcacaaaa tctgtttcgc
    21541 cactcacgta agaagccagc gcatgaatct tttattcaga attatgtcgc gattattgtc
    21601 ctgttttgcg ccagcggcac cgggatcttc ggggcgatga acgaagggat gaccggcgat
    21661 ccgagtattt taatcgccaa gtcatttctt gatttcttta cggcgatgat cttcgcctgc
    21721 tcgctgggta ttgcggtatc ggtgattagt atcccattac tgatcatcca gttaacgctg
    21781 gcgtgggctg ccgcgctgat attaccgctg accacaccgt cgatgatggc agacttcagc
    21841 gccgtaggcg gtttattgct gctggcaacc ggattacgca tctgtggcat taaaatgttc
    21901 ccggtggtca acatgcttcc agcactcctt ctggcaatgc ccctttccgc cgcctggacc
    21961 gcctggtttg cctgacaatg cgtgcattag cggcaaagtg atgatagatt gtgcagtctg
    22021 cagtaaattg aagaaatttg attgacgaga cgaggcgaat caggtttaat gcgccccgtt
    22081 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    22141 ccgagtccgg gcaccacttt cacctccaga ttcatctccc gaactccacg aaactcaagt
    22201 ataatcacat ctttacaagc aaactgaaca ttctgaactc caccagactc cactgaattc
    22261 tctctcaatc aagcgttcat tgggggcacc tgtgggggcc cattgtgttc agtcagtttc
    22321 aggagcctcc atatggcact tacagccaga caggtcgaaa cagccagacc taaagaaaaa
    22381 gactataaac tctctgacga acgtggttta tatctgctgg ttaaaaccac gggagcccgc
    22441 tactggcggc ttaaataccg gatagcagga aaagagaaaa aactgtccct cggcgtctat
    22501 cccgatgttt cccttgctga agccagaatc aaacgcgacg atgcccgaaa aattatctcc
    22561 gaagggggtg acccgggcga aaagaagcga aaggaaaaac tcactcagaa aatctctgcc
    22621 accaatacgt tccatgccct cgctacggaa tggcaccagc ataaatcttt atcatggtct
    22681 gaaagttacg ccagaagcgt actggaggcg ctggataaag atattttccc gtatctgggc
    22741 aaacgaagcg ttactgatat cctcccgctg gaaatgctgg aaattctgcg tcgcatagaa
    22801 aaacgtggct cactggaaaa actgcgtaag gtccgccagt actgtaatca gatttttcgt
    22861 tatgccatcg ccaccggacg agccactgtc aatccggcat ctgaactgac cagtacgctg
    22921 gcgacgccaa aagctgcgca tttcccccac ctgagagcag atgagctccc tgtttttctc
    22981 cggaggctcg ctgaatatca tggcagtcct gttacccgca tggcgacaaa tctgctgctt
    23041 ctgacaggcc tcagaacgat tgaactacgg tccgctgaat ggtcagaaat tgattttgat
    23101 aatgccctgt ggacaatccc tgaaagccgc atgaaaatgc gacgtaaaca tgtcgtacca
    23161 ctgtcacaac aggccactga cattctgctg cagctcagaa ccttctccgg acaataccgg
    23221 ctggtttttc ccgggcgttg tgatatcaac aagccgatga gcgaagccag catcaatatg
    23281 gtgctcaaac gtatcggtta cgatggcagg gcaaccggtc atggttttcg tcacaccatg
    23341 agcaccattc tgcacgaaca gggctttaat tcttcctgga ttgaaatgca gttagctcat
    23401 gtggataaaa acgccatcag aggcacctat aaccacgcac agtatctgga tggtcgccgt
    23461 gaaatgatgc agtggtacgc cgactacatt gattcactct ccagccagta aagttaagtc
    23521 agaaatactt ttccatgata ctttttcagg gttaagtatt ttaccctccg gcctgcaggg
    23581 ccggaggggt tttgttatta attatatttt ttcatctgca aaattatcac aaaaaacatg
    23641 attgcagaca aattgacatt ttcaggctgg tgattacaga atatggcctt gcacttattc
    23701 cggcaccttc agcaaaggaa aaattgattc agatattatt tagcatttca gtcatctgac
    23761 atcattataa aaaatgaaat tcatataaag ataagattat tgttatcaat gccaggcagg
    23821 cactggcttc gtaagtaatg atcgttcggg gccaggacag agcctgatgc gtattccgct
    23881 ctattcgatc acggattggc atcgaagtcc gatcagcgat tcgcattttt taccaaaacg
    23941 ctgctggagt gagagcttca tctgctcttc cagaatacgc atacggtcca gaaggccagc
    24001 attctcagcc ttgactttct gcaccatggc aatcgccagt gtgcgaagct gttccgggtc
    24061 acgggtggtg gtgagaagtg aaatatccat gcggtggatt atacaccatt gggcatggat
    24121 attcattaca tttcatgtgg ttactgtgat atgctgcgca tccggaggcg gttcccggta
    24181 tacccatttt gccaggtcat gccctttcac ctgtggccag tcaacgccac agacgagcca
    24241 gtgaaactca tccggtgtga gatgccatgt ggcatcacct ttccggggcc agcggaagct
    24301 tccccggtgc agtcgtcggg tacacagcca tacaccatgt ttgtcccacc ggagaacttt
    24361 tatccgggag cgggctttgt tggtaaagac aaaagcagct ccgtcatgcc atgctgcgtt
    24421 aagttcgttc tgaacgtact gggtcagggt gtcgataccg gcacgcatat cgacgggttt
    24481 cacggccagc cagacgttat caggtgtcag catggcgaag cgccctgaat acatcagcca
    24541 gttgcgctgc ccggcagcac agtcggatac caccgggcag atataacgaa accggttcgt
    24601 cagcgggggc agtcgcagtt gtcccccgac tctccggaag tccggatacg ctgaccggga
    24661 tgaaggcggg tccatgcgcc ttcctgtcgg cccgggcgat atctttcggc cagtagcgca
    24721 tggatttatg ggggatattg tgctgttcgc agtacgccat ccgggaaagt ccactggccc
    24781 gccatgcgcc aacatgttgt cttttttgct caagcgtcca tcgtgtatct ggcattgtca
    24841 ttttcctcca ggttgcagac atgtagcaat cctaaaggag tgaaattttc tgtgaagatg
    24901 agttcaccgg aacgttactg aagatgagtt caccggaacg ttacaacgcg atggcggaga
    24961 gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtgcag
    25021 aagtggaact ggccacactc acgtgggtgg actggtataa caatcgacga ttgctggaaa
    25081 ggctgggcca tactcctccg gcagaagcag aaaaagctta ttatgcttcc atcgaaaacg
    25141 atgatctggc agcctgagtt cacagataaa acactctcca ggaaacccgg ggcggttcac
    25201 ttactttcat tttctgcaca ttcaaagagg tgtaaatatc accttcacgg tctgaaacag
    25261 aaattatctg ggtatcagga caggagctgg ccagttctgt tgctttttta tatccctcta
    25321 tccaccgtcc tgtttctttc tcactgatat gtttcctgtg aagtgaatca ttagttattt
    25381 ctttacgact ttgtttctgg gttttgagtt cttttctgaa ccattgataa tcatcataaa
    25441 cccccaggca aataactgaa tcactagtaa ccatttgaga gtgaataaac aatctcgctc
    25501 actttcatgt cgttttacac cgatgccctg tttctttttt gtccagaatg atttgtttca
    25561 gtcgtatcct gtaaaaccag agcccgttaa actgtttagt cctttcgatg ttatttttct
    25621 tatggggagc cataatttta tcggctgaaa ttttatcact gaaaaatgca acgataagca
    25681 ccttttgtct cactccgggt gggcaggata cgggaattct ttcctttggt gcgcgactta
    25741 ccacactgag aatgtgcttt attcgctttt cgagcctgat agcatgaacg cacagagatt
    25801 ccagcccctt ataacccagt tttccatagt tcctctagat tccgtttaat ttatcgacag
    25861 tatagcttcc ccttggccga tgtttcatgc cttggtgctc aactgcgttc gatacccaag
    25921 gccatgtttg cactattgcc tatgatttta cctcttgtat cgttgttcac cgcacggcgg
    25981 caccaattcg cttggcgggt tatccggtcg gtatgagccg cgaccgggcg ccgtgaccta
    26041 ccgacgtttg acttcagttc cgacggccgc aatcggcccg tcccgatcag gtattgtgcc
    26101 catggtttta cggggtcagt gtaaggaacc gtttgtgcgg ggacggcgac gagcggccac
    26161 tttagctttg gtcttcgttc gcattgtcat cctgcggtgg tccatcgttt agatttatcc
    26221 ggttaagggc aaccgggatc tcgcatggga tttacgatta caacggcatg tattcttccc
    26281 cactgtacgg tgtcctagag tggatcaacg ccgcagattc cttttctgat ggtgatcatc
    26341 gtagcatttt gtgagggaga cagtgtttga gttcgcatgg tctcaatccc gtcggcttaa
    26401 tatttttttg tctagtaact ttgttgattt tagagttttg ttcccagaca gtgttgtatc
    26461 tttggcattc tgtttggctc tccggaacca ccggcccatc cccgttcgtc ggtctatggg
    26521 tcgggtatcg tggtggagcg tgacggtaac cgtatggtgg accggctatg gtgccaccct
    26581 ttcggctccg ggtcgcgttc atctgtaccg acgttcgtga ccgttattgg ggcagtggtc
    26641 ctgccccgta cctcttcgtg gtcctatgtt ctacgcaggg tcagcgcgat gccctcaact
    26701 cacccagtag cccccgtcca ggttcagtat gtaggacccg gccggtttgt ccacgttgtc
    26761 cccgtccacg actacgaaca agttgccacc ccagttccca gcatcggcgc ggtatacggt
    26821 gtcgccgttg gtattccacg cactcccgtc ggaggactgc aagcggacat ttatccgtgc
    26881 ctgctggtca tcgcggagca gtacgatctt gccctggtgg tagttgtctg gctgtgcacg
    26941 caacgctgca tgctcggtgt tacagccttt tgcggttacg gacaggcccc ccacacgggc
    27001 cccgtcggtg agttctccgc gcgttttggt ggcgtcagat ccggtgacca gcacttggca
    27061 ctgttcggta acggttagtt ttgtcgtacc ggtgctacca cttcctgtga acgctgcatg
    27121 cgaggagctc atcgcgagga tagtgctggt cgcggccatg atcgctaatt ttttcatatt
    27181 gattacctgt tcagttttgt gagggggtaa cacaacagta cggtctaatt catcccagaa
    27241 tcagtaagct gtcttacccc acattaacca ccgttcacag atgtctgcac ccgcagcaga
    27301 gactgttcat tgcatataag taagtttctg actgcagggc ttgctcataa agccgcagac
    27361 aacgttcacg gtgatgcctt attccacggt ctgccagtcc ggtggccccg cgttcagccg
    27421 gtgttcgcca ccacgatgcg cctgaccagt gcgcgtcatc tgtgtgcttc ccggacgacg
    27481 taattcattc ataccggcgc tgcgtttttt acctttatat aaaccacgac ttcccactgg
    27541 cagattaccc ataataaaac ctccacctta tttctgattt aaatgattct gactgtttaa
    27601 attaaatccg gatggccgat ttatatttat gtaataaacc gccagcacgg attaactgcc
    27661 tgtgttaatc agctttgtgc tttccttctc tggtctttta tttttccttt attctttcct
    27721 gtattccctg cgtgccccct ccgggttgca ggtatccctc cacctcttct gcggaataca
    27781 agtcacggtc catgcgtgaa tcagtgttta tttcatttca gttaatttat ccggtgaaat
    27841 aaatactctc tgtgttttct gctttgtttt tgttttatgc tgtgttgtgc ttttgtcttt
    27901 ttcttctgtt tttcttctgc gctttattcc tgtggcacca cacaggctcc gccaaccgaa
    27961 aaccgatatt caccaggagc aatgtcctga tttccgtccg ccatcacatc aaaaaaaacc
    28021 tgctcctcct gaccgggcct cgacaccccc ttcccgcctt ctcccttcac cggcgaccag
    28081 ccatctcctc ctgtcctgac acgaagctca tgacgaccat ccttactctg caccacatag
    28141 cgctccgccc tgccgtccac ctgacgctca ttcatccaca catgaaaacc cgtgtgcgcc
    28201 tcccggcaga taatccgccc cgtcgccacc ttcgcaccat cgcgcagctg cgttcctgaa
    28261 cctccccggc tctccaggtg gagctcagcc gcctgcgaca caccactcat gaccatcagt
    28321 aacatcccgc aggtgacacg catcatcttc cttatactcc cgttcatctc cggctcctcc
    28381 cgtcacatac acaccgtacg ggtcaggaat atccccgcag gccccttctc ttccggaaga
    28441 cggtaatccg catggcacag actgttctct ccccactgcg ccttcagctt accgctttcc
    28501 ttcagcccgc tcaggtacac ctcaccacgg tctcccacca caccggctga gcccgcagcc
    28561 tgacccgctt cgccctcaac tgtcaccacc gcaccaaacg gcagaggcgt tccgtcctga
    28621 cgggccagac tcaccagcgc acgaccaccc acacgggtcc tgaatccggc ccggaccact
    28681 gcccccttcg tcggcaccac acgcctgtcc gtctgcggca cttccgcatc ctccggaaac
    28741 gtcgtcgggt ccagtgtcag cacgttctcc tgatacggtg acgcataacc ggccagcgta
    28801 tacccccgga aatccgttct cacacccggc cagccaccca cagacgcacc actcacgccc
    28861 ggtgccgcaa tcagtgccac cgtatccccg gtccgctgac caaaggtgac cccctcactg
    28921 tgggccacca tgctgccgga catcccggcg ttcagctggc gcatcgtgct gctgtaactg
    28981 tacatccccg tcagttcacc atatgtcccg taccacgtca ggttcagacg actggtgtca
    29041 gcatggctct cactgcccgg caccatctgc tcacggacat cccagtacag ccggcggtca
    29101 aatgcccgtc cgttcagccc ggcctcatac cgggtatcct gtcccgtgga acgctgcatc
    29161 tgcgccgtgg cactgatgtc gttatccgtc cccccaaacc agcgtcccag cggcacactc
    29221 atccacatac tgacactgtc ttccgtacgc gaccagccac cgtaatagcc gccgctgttg
    29281 cggttgcgtg accagttaac cgacagcgag atattgttcc aggacgtact gtatgacgcg
    29341 ccaaaataat cacggtgcgg tctgtcacgg tactcatccc ggctgccgtt cagtcccaca
    29401 tagccccact gacccaggga ctgactcaga ctcagcgtgg tacggcgact ccggttctcc
    29461 gtgtgacggt atgcgtaccg gtcatcacgc caggtgtcca gcacgtccgg cagcgtatgg
    29521 taaccatccg atgagtactg ataactcgct gccgtaaaac tcgtcccctt cagctcaaac
    29581 gacttgttat aacggatacg ccaggtgtca ccggtctcat aatcatgtcc cttctgctgc
    29641 ccccgggagt gcgtcgtgtc cagcgacacc gcccccagac gccccagtga ccagccaagc
    29701 cccagagccg cagactggta atgctccgct ccctgaagcc ccccgtacac cgtcagcccc
    29761 cacggcagac cgtacatggc cgtcacctgt cccagcgacg tgtgctcaac cgcatcatcc
    29821 gatgaacggt actgacctgc cgtgacgttg tacttcaggt acccctcacg cagcgcaatg
    29881 gccggcgtgg tgaacggcac cgtgaaaacc tgcgccgtcc cgtctgattc cagcaccgtc
    29941 acctgcaggt cactgccggc ccccgtcacc ggcaggtccg tcagggcaaa tgcccccggc
    30001 gccaccgtct gactctgtat cagatacccg ttctgacgca cctctatccg cgcctgtgtc
    30061 cgggcaatgc cacgtacaac cggcgcaaat tcacgcaggt tataaggcac catgctctca
    30121 tcagaactca tcatcgcacc ccggaaaggc acactgtcaa aaatgtctga cggcgtgtaa
    30181 tcctcaccca gcgtcaggcg actctttatc ccgttcagcc cccgctcagc acgtatgtat
    30241 gaactctccc attttcccga ctgaccggat gacctgttcc aggtggtcag gttcctaaca
    30301 cgccagggtc caatgttgat tcccggctgc agactgaccc agtaactgtc cgtgacacgc
    30361 ttcccgtaac cccggtactc actgcgcccc gcattcgcct gccagttcag cagaaaagcc
    30421 ggaatgccat catcccataa cgcctccggc gcaatccccc tcagctgcgg acgcagtgcc
    30481 acctgaggga tacccagaac cagctgctgg gcagcaaact gataactctc cgtggcctgc
    30541 ggtatcgccg tcaggtcagc acaggcctcc tccgccacgg aggcaccacg accctctccg
    30601 gatgcacgga acaacgccgg atattcctca atcctgaccc cgtaacgcgc cagcatctca
    30661 cgggtcagac aggtcttcag ataaggcctg ccctccgcgt ccctctccgc gtgaaaggcc
    30721 atctcctgtg aatccacacg ggaaccattc aggataatgt caaccggata aatgccgggt
    30781 aactgcccac cttcctcaaa cagggtcagg tccaccccct taccgccacc tttcagcatg
    30841 gccgcatcaa aggagtaggt gcgcgcaata ccactctccg cacgaaacat cacacaggcc
    30901 accatcacag ccagcgccag ccctgtcacc cccgttttca tccgccctga agaagtatca
    30961 cgcatctgtc agcccgccat ttaccctgtt atcaccttat gtattcaacc cttcagctct
    31021 gcctcaaact gcttactggt cccgccgtaa tccgttatca ccttccactg aaccttaccc
    31081 gatgcccccg caggcagcgg atattcacgg gaggaaaacg gcgcaatata ttcacgctcc
    31141 ttcacttcct taccccccac cgtcagcgtg gacaggttga tgtaaaacgg cgtcgggtta
    31201 acccccttca gcctgttgcc ggccctctgc cactccacct tgccggccac atcatccggt
    31261 cgccccttca ccgccggcgg acgaacaaac agcttgatgc agctgctcac tgaaagctgt
    31321 acattcaggg agactttgtc agccttcttc tccccgtcct tcccttccgc ccacctgtca
    31381 tcctccttcg gcggaatgcc tttcacgcaa atccactgca gactctcacg gtctgacgga
    31441 aactccccgc ccgtcctgac aatacgcaga cgactcgact gctgaccatc aagacggaac
    31501 aacggtggtg tcaccacaaa aggcgccgga ctcttctggt cctcactcag cacctccgac
    31561 tgcaccagca tcggatagtc ctggtcatta atcaccgtca gcgtctcccc cgacgaggcc
    31621 gggttgtaaa ccacccgcgt ggcccccaga tgcagcgaga atactctggc gtttgtctcc
    31681 gtcgcggatg cagacatatt cccttctccc ccggcggcat atgcccccgc tgacagcaaa
    31741 cctgtcactg ataacactcc cgtcagcatg ccggtgaaca cagccacagc ccgcattttc
    31801 atactccctc cggaacagac agatacacgt tgtcttttat atatattcat tcaccatccc
    31861 cacccggaac tgactctgac cggactgcat gcagtcaccg cattcgtgcc gggtggcgaa
    31921 acggatggtg aaactgatgt taatattca
//
LOCUS       DAZFRE010000057        31107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFRE010000057 DAZFRE010000000
VERSION     DAZFRE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.057"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(207..1076)
                     /locus_tag="ACHKUI_002935"
     CDS             complement(207..1076)
                     /locus_tag="ACHKUI_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6599097.1"
                     /translation="MTLQRNGAQSVSGTTNASGSVNLGSTFADDQDALLIVKKEGYSN
                     LVVKCPCAGMTYAISPAMTSLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDA
                     NLDVDDTDSYGPETVTISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSS
                     LVRSYSVPAGKRGNIWTMFKLNPNGEFEDINSVTSANFNDTTLDVRDLATVIMPATGT
                     SASASPAMQNSGDTQLARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLG
                     LAYQKNGNIAEAI"
     gene            complement(1406..2275)
                     /gene="sucD"
                     /locus_tag="ACHKUI_002936"
     CDS             complement(1406..2275)
                     /gene="sucD"
                     /locus_tag="ACHKUI_002936"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6599098.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(2275..3441)
                     /gene="sucC"
                     /locus_tag="ACHKUI_002937"
     CDS             complement(2275..3441)
                     /gene="sucC"
                     /locus_tag="ACHKUI_002937"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6599099.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(3703..4920)
                     /gene="odhB"
                     /locus_tag="ACHKUI_002938"
     CDS             complement(3703..4920)
                     /gene="odhB"
                     /locus_tag="ACHKUI_002938"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6599100.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4935..7736)
                     /gene="sucA"
                     /locus_tag="ACHKUI_002939"
     CDS             complement(4935..7736)
                     /gene="sucA"
                     /locus_tag="ACHKUI_002939"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6599101.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(8192..8908)
                     /gene="sdhB"
                     /locus_tag="ACHKUI_002940"
     CDS             complement(8192..8908)
                     /gene="sdhB"
                     /locus_tag="ACHKUI_002940"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6599102.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8924..10690)
                     /gene="sdhA"
                     /locus_tag="ACHKUI_002941"
     CDS             complement(8924..10690)
                     /gene="sdhA"
                     /locus_tag="ACHKUI_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6599103.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(10690..11037)
                     /gene="sdhD"
                     /locus_tag="ACHKUI_002942"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(10690..11037)
                     /gene="sdhD"
                     /locus_tag="ACHKUI_002942"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6599104.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(11031..11435)
                     /gene="sdhC"
                     /locus_tag="ACHKUI_002943"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(11031..11435)
                     /gene="sdhC"
                     /locus_tag="ACHKUI_002943"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6599105.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11816..11923
                     /locus_tag="ACHKUI_002944"
     CDS             11816..11923
                     /locus_tag="ACHKUI_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599106.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            12129..13412
                     /gene="gltA"
                     /locus_tag="ACHKUI_002945"
     CDS             12129..13412
                     /gene="gltA"
                     /locus_tag="ACHKUI_002945"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6599107.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            13539..13934
                     /locus_tag="ACHKUI_002946"
                     /pseudo
     CDS             13539..13934
                     /locus_tag="ACHKUI_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            14086..15132
                     /gene="abrB"
                     /locus_tag="ACHKUI_002947"
     CDS             14086..15132
                     /gene="abrB"
                     /locus_tag="ACHKUI_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6599108.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(15129..15920)
                     /gene="nei"
                     /locus_tag="ACHKUI_002948"
     CDS             complement(15129..15920)
                     /gene="nei"
                     /locus_tag="ACHKUI_002948"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6599109.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQY
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(15956..16690)
                     /gene="pxpA"
                     /locus_tag="ACHKUI_002949"
     CDS             complement(15956..16690)
                     /gene="pxpA"
                     /locus_tag="ACHKUI_002949"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6599110.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(16680..17612)
                     /gene="pxpC"
                     /locus_tag="ACHKUI_002950"
     CDS             complement(16680..17612)
                     /gene="pxpC"
                     /locus_tag="ACHKUI_002950"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6599111.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFESDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(17606..18262)
                     /gene="pxpB"
                     /locus_tag="ACHKUI_002951"
     CDS             complement(17606..18262)
                     /gene="pxpB"
                     /locus_tag="ACHKUI_002951"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6599112.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(18285..19028)
                     /gene="ybgI"
                     /locus_tag="ACHKUI_002952"
     CDS             complement(18285..19028)
                     /gene="ybgI"
                     /locus_tag="ACHKUI_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6599113.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLEVDAVIVHHGYFWKGESPVIRGMKRRRLKTLLANDINLYGWHLPLDS
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GSELVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            19299..20780
                     /gene="dtpD"
                     /locus_tag="ACHKUI_002953"
     CDS             19299..20780
                     /gene="dtpD"
                     /locus_tag="ACHKUI_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6599114.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAIDY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(20822..22240)
                     /gene="phrB"
                     /locus_tag="ACHKUI_002954"
     CDS             complement(20822..22240)
                     /gene="phrB"
                     /locus_tag="ACHKUI_002954"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6599115.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(22237..22746)
                     /gene="ybgA"
                     /locus_tag="ACHKUI_002955"
     CDS             complement(22237..22746)
                     /gene="ybgA"
                     /locus_tag="ACHKUI_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6599116.1"
                     /translation="MNQQLFDDSTIIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHAQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(22847..23053)
                     /gene="ybfA"
                     /locus_tag="ACHKUI_002956"
     CDS             complement(22847..23053)
                     /gene="ybfA"
                     /locus_tag="ACHKUI_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6599117.1"
                     /translation="MELYREYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            23366..23455
                     /gene="kdpF"
                     /locus_tag="ACHKUI_002957"
     CDS             23366..23455
                     /gene="kdpF"
                     /locus_tag="ACHKUI_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6599118.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            23455..25128
                     /gene="kdpA"
                     /locus_tag="ACHKUI_002958"
     CDS             23455..25128
                     /gene="kdpA"
                     /locus_tag="ACHKUI_002958"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6599119.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     NTLGNAWVDLLRITLWVLTPVALLIALFFIQQGALQNFLPYQAVTTIEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSYTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            25151..27199
                     /gene="kdpB"
                     /locus_tag="ACHKUI_002959"
     CDS             25151..27199
                     /gene="kdpB"
                     /locus_tag="ACHKUI_002959"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6599120.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            27208..27780
                     /gene="kdpC"
                     /locus_tag="ACHKUI_002960"
     CDS             27208..27780
                     /gene="kdpC"
                     /locus_tag="ACHKUI_002960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6599121.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTSNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            27773..30457
                     /gene="kdpD"
                     /locus_tag="ACHKUI_002961"
     CDS             27773..30457
                     /gene="kdpD"
                     /locus_tag="ACHKUI_002961"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6599122.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDAPLARTLNNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            30454..>31107
                     /gene="kdpE"
                     /locus_tag="ACHKUI_002962"
     CDS             30454..>31107
                     /gene="kdpE"
                     /locus_tag="ACHKUI_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6599123.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     "
BASE COUNT         7002 a         8397 c         8109 g         7599 t
ORIGIN      
        1 gaaaaacaag caagaatata aaatttaaaa atatgattcc actttcattg ttataaaatg
       61 gcatcgtgaa gagcgagttg ataatgctgc aatgcatcag cgtcctgtcc agcagttcca
      121 taaattttgg caatgttata ataggagtta gcgcgcgttg tcgccgcatt tgctccacag
      181 gctaacgaga tcgctttacg atttgcctaa attgcttccg ctatgttgcc atttttctga
      241 tacgccagcc ccaggttact aaatgcctgt ccgtaattgc cgtctaattc tgttgcctgt
      301 tggaagagtt gaatggcttg ctcaagttga ccggttttat aaacagcttc accttcgcga
      361 ttatattttc ttgccagttg agtatctcca ctgttttgca tcgcaggcga agccgaagct
      421 gaagtgcccg tcgcaggcat tatcacagtg gctaaatccc tgacgtctaa tgtggtgtcg
      481 ttaaaattag cgctggtaac agagttaata tcttcaaact cgccgtttgg attgagttta
      541 aacatggtcc agatgttccc tcgttttcca gcagggacac tataactacg caccagggag
      601 ctccctacat aaacaaacac ttttgcctta ctggcagaga gataattact gttgggcaac
      661 cctttattgc tatagtcctg cacggcatag atgtaacttt cgccaaaatg ctttttgctg
      721 atagtgacag tttctggtcc atagctatcc gtatcatcaa catccagatt agcatcagtc
      781 ccctctttac tatcaaaata gatatgtccg ccagggaaga tcaaatgtga atcaagatca
      841 aaaggctttt cgccccagct caaaactaca cgcatgccat ccagactagt cattgccgga
      901 ctaatcgcat aagtcatgcc agcacacgga catttgacca ccagattaga atagccctct
      961 tttttgacga tcaataaagc atcttgatca tcagcaaatg tagaacccag attaacgctg
     1021 cccgatgcat ttgtggttcc agaaacagat tgtgcgccat tgcgttgcag cgtcatcgat
     1081 gctccctcaa tgcgtttatc tttcactgtc gcacttaaaa tatcgatatt tacatcggcc
     1141 gcataaacgt ccgaatttat acccatcagg gccagagcaa ctggcagaac aattttcttc
     1201 ttgtataaat gcttttacag aacggagagg gaatattttg ccgggcatta ttgcgcagaa
     1261 gatgggaaaa aagcagtggg caaacttttc agattagtgc ccgaataaaa aaggccatca
     1321 taagatggcc aaccatgtcg aaaccgacag cccttcgccc ctcctgtaga gggaagcggc
     1381 gagggctatt tcttattaca gatatttatt tcagaacagt tttcagtgct tcaccgatat
     1441 ccgccaggct gcgaacggtt ttcacgcctg cggcttccag agcagcgaat ttctcatcag
     1501 cagtcccttt accaccggca atgatcgcac ctgcgtgacc catacgtttg cctttcggcg
     1561 cagtcacacc agcgatgtaa cccacaactg gcttggtaac gtgctccttg atgtacgccg
     1621 ctgcttcttc ttcagcgcta ccgccgatct caccgatcat cacgatcgct tcggtctgcg
     1681 gatctttttc gaacatttcg aggatatcga taaagttaga gccagggatc gggtcgccgc
     1741 cgataccaac acaggtcgac tgaccgaaac cgtaatccgt ggtctgttta accgcttcat
     1801 aggtcagcgt accggaacgg gaaacgatac ccactttacc cggtttgtga atgtgacccg
     1861 gctggatacc gattttgcat tcccccgggg tgataacgcc tgggcagttc gggccgatca
     1921 tacgaacgcc tgcttcatcc agtttcactt tcaccgtcag catatccagc gtcgggatgc
     1981 cttcagtgat ggtgataatc agtttgatgc ctgcatcgat agcttccaga atggagtctt
     2041 tgcagaacgg tgctggtacg tagataacag aggcggtagc gccagtagca gcaacagctt
     2101 cacgcacggt gttgaacacc ggcaggccga ggtgggtagt accgccttta cctggggtta
     2161 cgccgccaac cattttagtg ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc
     2221 taccggtaaa gccctggcag ataaccttgg tgtttttatc aattaaaatg gacattattt
     2281 cccctccact gcggcaacaa cctgctgagc tgcatccgtc agaccttttg ctgcaataat
     2341 attcaggccg ctgtcagcca gtttcttcgc accgagttcg gcgttgttac cttccagacg
     2401 taccacgacc ggtacgttaa cacccacttc tgctaccgcg ccgatgatac cgtcagcgat
     2461 caggtcgcaa cgaacgatac cgccgaagat gttaaccaga acggctttca ctttgtcgtc
     2521 agagaggatg attttgaacg cttcggttac acgttctttg gttgcgccgc cgccaacgtc
     2581 caggaagtta gccggttcgc cgccgtgcag tttaacgatg tccatcgtac ccatcgccag
     2641 acctgcgccg ttaaccatac aaccgatgtt accgtccagc gcaacgtagt tcagttccca
     2701 ctgtgcagcc tgtgcttcac gcggatcttc ctgcgactgg tcacgcattt cacgcagatc
     2761 aggctggcgg aacagtgcgt tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc
     2821 gccctgtttg gtaataacca gcgggttgat ttcgataagc gcaaggtcgc gctccaggaa
     2881 aatggtcgcc aggcccatga agattttggt gaactgctga accagtttac cttccagacc
     2941 cagtttaaac gccagttcgc gtccctgata cggcatcggg ccagtcagcg gatcaagcgc
     3001 aactttgtgg atcaggtgcg gagtttcttc cgccactttt tcgatttcca cgccgccttc
     3061 ggtggaggcc ataaagacca cgcgacggga actacggtca acaacggcac cgagatacag
     3121 ctctttagcg atatcggtcg ctgcttcaac cagaatctgg ttaaccggtt ggccattagc
     3181 atctgtttga tacgttacca gacgcttgcc cagccagttt tctgcaaaag cacggatgtc
     3241 ttctttgctg tttacaactt tcacaccgcc cgctttaccg cggccaccag cgtgaacctg
     3301 acatttcact acccacggac cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg
     3361 agtagtacag gcataaccca ccggtgctgg taagccatag cgggcaaaaa gttgttttgc
     3421 ctgatattca tgtaagttca tgtgttctgt ccatccttca gtaatcgtta tcttttaaac
     3481 cgtagaccga atatgacgta tcgcgtccgg gtactgccgg tcacccgtag gcctgatgtc
     3541 gcatcagaca tcgcttgtcg gatgcggcgt aagcgcctta tccgacctac attggtgatc
     3601 accgtaggcc tgataagacg cattagcgtc gcatcaggct tcgattgccg gagaagtcaa
     3661 tattgtctta tccggtctac agtgcaggtg aagcttaaac tactaaacgt ccagcagcag
     3721 acgcgtcgga tcttccagca actctttgat cgttaccagg aagcccacgg attcgcgacc
     3781 atcgatcaga cggtgatcgt aggacagcgc caggtacatc ataggcagga tctcaacctg
     3841 accattcact gccatcggac gatctttgat agcgtgcata cccagaattg cgctctgcgg
     3901 cgggttgatg ataggcgtag acatcaggga accgaacaca ccaccgttgg tgatggtgaa
     3961 gttaccaccg gtcagatctt caaccgttag cttgccgtca cggcctttaa cagccagctc
     4021 tttgattttc ttctcgatgt ctgccatgcc gagggtatcg acatcacgca gaaccggcgt
     4081 caccagaccg cgcggcgtag aaaccgccat actgacgtcg aaatagttgt ggtagaccac
     4141 atcatcgccg tcgatagacg cattcacttc cgggtaacgt ttcagggctt caaccaccgc
     4201 tttcacgtag aaggacataa agcccagacg aatgccgtgg cgtttttcaa acgcttcacc
     4261 gtactgctta cgcagatcca taatcggctt catgttgact tcgttgaacg tggtcagcat
     4321 ggcggtggag tttttcgctt ccagcagacg ctctgccaca cgcttacgca ggcgggtcat
     4381 cggcacacgt ttttcgctac gtgcagccag ggccggttgc gccgccggag cagccgctgc
     4441 cggagcagac tctttcgccg gggctttcgc cagatgtttt tccacatctt cacgggtcag
     4501 acgaccaccc acacccgtgc ctttaatggc gctggcgtcg aggttatgtt cagccagcag
     4561 gcgacggatc gccgggctta acgcatcgtt gttttgctct tccagagacg cctgctggcg
     4621 ttgcgccgga gtggacgctt tctcttcaga tttggcgctg gtttctttac ccgcgctgtt
     4681 gccttcacgc aggcgaccaa ggatctgacg agacgttacc gttgtacctt catcttccag
     4741 aaccgcatcc agaatgccgt ctgctgatgc cggtacttcc agtaccactt tgtcagtttc
     4801 gatttctacc agcacttcat cacgtacgac tgcgtcgccg ggttttttat gccaggttgc
     4861 gacggtggca tcggctacgg attcaggcag gtcagggacc agaatatcta cgctactcat
     4921 tgtgtatcct ttatttattc gacgttcagc gcgtcattaa ccagatcttg ttgctgtttc
     4981 tggtgaacgg acatataccc taccgccgga gaggcggagg ctgggcggcc tgcataacgc
     5041 agagaagccc caaacggaat cacttcacgg aaatgatgct ggctgcagta ccatgcgccc
     5101 tggttgagcg gctcttcctg gcaccagaca aaatccttga cgtgagcaaa ctgctgcaac
     5161 acttcctgca tcgctttatg cgggaacggg tagagttgct cgatacgcac aatggcgaca
     5221 tcgtgttgat tgttcttacg acgttgttcc agcaggtcgt aataaacctt accagaacac
     5281 atcactacgc gcttcacgcc cttcggatca agctcgtcga tttcaccgat ggctggcagg
     5341 aaggtgccgt tcgccagttc ttcgaggctg gataccgcca gcggatgacg cagcagggat
     5401 ttcggcgaca tcacgaccag tggacgacgc atcccgcgca acgcctgacg acgcagcatg
     5461 tggtaaacct gtgccggggt agaaggtacg caaacctgca tgttttgctc agcgcaaagt
     5521 tgcagataac gttccagacg cgcggaggag tgctccggcc cctgcccttc gtaaccgtgc
     5581 ggcagcaaca tcaccaggcc acacatccgg ccccatttct gttcgccaga ggagatgaac
     5641 tggtcgataa ccacctgtgc accgttggcg aagtcaccga actgtgcttc ccagatggtc
     5701 agggtacgtg gttctgcggt ggcataaccg tattcaaacg ccagtacggc ttcttcagac
     5761 agtacagagt cccagacgcg gaacgcgccc tggccgttat ggatatgttg cagcggcgtg
     5821 taagtggaac cgttagactg gttgtggatc accgcgtggc ggtggaagaa ggtaccgcga
     5881 ccggagtctt cacccgacag gcgaaccgga atgccttcgt caaccagcgt ggcgtaagcg
     5941 aggttttccg cgccgcccca gtcgaacagt ttctcaccgg cagccattgc ctggcgatcg
     6001 ccataaatct tggcaacgcg agactgcatt tcaaccgctt ccggcaccgt gctgatgcgt
     6061 ttagccagtt cctgcaggcg cttcatctca actttgttcg ggtactcttc gtcccattcg
     6121 tggttgaggt acggcgacca ggtgaaagag tgcatgttca tcggacgcca ctctgctaca
     6181 acgcaatcgc cagcatccag cgcatcgcgg tacaggttaa ccatctcggt ggcatcttcc
     6241 agcgtcgcga ctttttcctg ctccagcttg tcagcgtaga ttttgcgcgg cgtcggatgt
     6301 tttttgattt tctgatacat cagcggctgg gttgcgctcg gctcgtcggc ttcgttgtgg
     6361 ccgtgacggc ggtagcacac cagatcgatg aagacatcac gtttaaaggt gttacggaaa
     6421 tcgagcgcca gacgggtcac aaaggcaacg gcttccggat catccgcatt aacgtggaaa
     6481 atcggtgcct gtaccatctt gccgatatca gtacagtacg gcgtagaacg ggcatccagc
     6541 gggttagagg tggtgaagcc aacctggttg ttgataacga tacgtaccgt accgccaact
     6601 tcataaccac gcgctttcga catgttcagg gtttcctgaa ctacgccctg cccggtcact
     6661 gcggcgtcac catgaatggt gattggcagc actttgttgc tgctcggctc atcaagtctg
     6721 tccagacggg cacgaacaga accgataacg accgggctta caatctcaag gtgagacggg
     6781 ttaaacgcca gcgccaggtg aaccaggccg ccatcggtct ggaagtcaga cgagaagccc
     6841 atgtggtatt tcacgtcgcc cgtgccgagg tgttctttat gtttaccggc aaactcgtcg
     6901 aacaagtctt gcggtttttt acccagcacg ttcaccagca cgttcagacg accacggtgc
     6961 gccattccga gaaccacttc gcgggtgccg ctgttgccag cgtggcggat catctcttta
     7021 agcatcggga ttaacgcgtc accgccttcc agcgagaagc gttttgcgcc agggaatttt
     7081 gcgccgaggt aacgttcaag gccttcagcg gcggtcagtt cgcttaagaa gcgttttttc
     7141 tcttcgctat tgaaagtcgc gcgaccagac tcaatacgct gttggatcca gcgtttttct
     7201 tcagtgctgg taatgtgcat atactcggca ccaatcgggc cgcagtaggt ttgcttgagt
     7261 gcttccagca gttcgccgag tttcatggtt tctttgccgc tggcaaatga accgacgttg
     7321 aaggtctcct ggaagtctgc ttcggtcaga tcgtggaaag acggatccag atcggccact
     7381 ttatcttgct gccacagtcc cagcggatcg agattcgcat gctggtgacc acggaagcgg
     7441 tatgcgttaa tgagctgcag gactttaacc tgcttcacat tggtgtcagg gtcggagatc
     7501 gttgaagagt aacgtgaagc gtctttcgcc aggcggcgga aatattcacg cgtttgagag
     7561 tggaattgat ccggtttgac tcccgtacca ggtaactgct ggaacgtcga acgccagtta
     7621 gcgtcaaccg agtcaggatc ggttaagaag tcttcataga gctgttctat ccagctctgg
     7681 tttgcgccag agaggtaaga agagtccaac caggctttca aagcgctgtt ctgcatcgtg
     7741 atcccttaag catctttttt atgcttactt cgccgtggat actaccacgc acatttcata
     7801 tctgatgtgc gtgccggggt tcacttgttg cggacttgtc gtgttttctg cgtccgctaa
     7861 ggaaccttta aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt
     7921 ttgtaggccg gataaggcat tcacgccgca tccggcgctg tttgcctgat gcgacgcttg
     7981 cgcgtcttat caggcctacg gtttctgcat ttcttttgta ggccggataa ggcgttcacg
     8041 ccgcatccgg cactggttgc ctgatgcgac gcttgtgcgt cttatcaggc ctacggtttc
     8101 tgcatttctt ttgtaggccg gataaggcgt tcacgccgca tccggctctg gttgcctgat
     8161 gcgacgcttg cgcgtcttat caggcctggg tttacgcatt acgttgcaac aacatcgact
     8221 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
     8281 tgatgctgtg acagcggaat acactgaatg catcgctcaa accgtcgagg cggctgtcag
     8341 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
     8401 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
     8461 tacattcata caacccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
     8521 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
     8581 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
     8641 caatcttctt gcccggctgg ttgagtgccg aaatcggagt aatacaggcc agaccattct
     8701 taccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
     8761 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcacgac
     8821 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
     8881 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
     8941 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
     9001 ccgactctgg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
     9061 cgaagcggct atgcgcgcca cggctttcgg tgcggaagtt ggcagaaaca gccgttgcat
     9121 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
     9181 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gataactttc aactgctcaa
     9241 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
     9301 gcagtgcttt acggatcgcc accggatctt caccgttacg gttattgttc cagcggttca
     9361 ggcgatccag agacgcttct acatcagact cgctggcatc gcgcagtgcg ccctgctcgg
     9421 cgatagactc ttgcagatgt agacctgccg cacgaccaaa tacgaccagg tccagcagcg
     9481 agttgccgcc cagacggtta gcgccatgta ccgatacaca agcgatttca ccaacggcaa
     9541 acagccccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtca
     9601 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
     9661 tcaccggatc gacgtgagcg aaggtacggg agagttccag aatacccggc agacgggatt
     9721 cgagaacttc tttccccaga tggtcaagtt tcagttttgc gtgtggcccc cacggaccat
     9781 cacaaccgcg accttcacgg atttcgatca tgatggaacg cgccaccacg tcacggcccg
     9841 ccaggtcttt ggcgttcggc gcataacgtt ccataaagcg ttcgccatgt ttgttcagca
     9901 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaataccgg
     9961 tcgggtggaa ctgccacatt tccatatcct gtaccggtac gcctgcacgg atagccatgc
    10021 cgacaccgtc gccagtgtta atgtgggcgt tggtggtgga ctgataaata cgccctgccc
    10081 cgccagtcgc cagcactgtc gcgcgagctt taaagtaaac cacttcacca gtttcgatgc
    10141 acagtgcggt acaaccgacc actgcgccat cctggttttt caccagatcc agcgcatacc
    10201 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    10261 cgtgaccggt acggtcggca gccgccgcag tacgtgccgc ctgctcgccg ccgaagtttt
    10321 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcaagacgc gagaacggca
    10381 ggcccatatg ttccagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    10441 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    10501 tatcttcatg ggtattaccc agcgcaacgg taataccacc ttgcgcagaa acggtatggg
    10561 aacgggtcgg gaagacttta gagagcagcg cacaggtctg gccactctgg gaaatttgca
    10621 gcgccgcgcg catacctgcg ccgccagcac caatcacaac tgcatcaaat tctctgactg
    10681 gcaatttcat cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    10741 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    10801 cacctgccac atgccgatcc aggcatggat caagatagaa aacagcgcca gcagggtgaa
    10861 cactttggtg aacgcagagg cgaagaaacc aatccagact tcatatgtca gttcgccact
    10921 ggtagcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcacg
    10981 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    11041 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    11101 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    11161 acgacgacgt gatatgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    11221 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    11281 agccacagca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    11341 gacgctatcg ccgtgacggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    11401 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt cccaggggac
    11461 tacgggcaca gaggttaact ttctgttacc aggagacgtc gggatttcct tcctccggtc
    11521 tgcttgcggg tcagacagcg tcctttctat aactgcgcgt catgcaaaac actgcttcca
    11581 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    11641 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    11701 gttttatccc gaacagtgat ctgcgtcacg ataacaacat ttatttaatt tttaatcatc
    11761 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    11821 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    11881 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    11941 tacttgattc gccatttatt cgtcatcaat agatccttta cctgcaagcg cccagagctc
    12001 tgtacccagg ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    12061 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    12121 gaccttaaat ggctgataca aaagcaaaac tcaccctcaa cggggacaca gctgttgaac
    12181 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    12241 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    12301 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    12361 cgaccgattc taactacctg gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    12421 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    12481 ttacccgtct gttccatgct ttccgtcgcg actcgcatcc aatggcagtc atgtgtggta
    12541 ttaccggcgc gctggcagcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    12601 gtgaaattgc cgcgttccgc ctgctgtcaa aaatgccgac catggccgcg atgtgttaca
    12661 agtattccat tggtcagcca tttgtttacc cgcgcaacga tctctcctac gccggtaact
    12721 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    12781 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tccacctcca
    12841 ccgtgcgtac cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg
    12901 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    12961 aaatcagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagac aaaaatgatt
    13021 ctttccgcct gatgggcttc ggtcatcgcg tgtacaaaaa ctacgacccg cgcgccaccg
    13081 taatgcgtga aacctgccat gaagtgctga aagagctggg caccaaagat gacctgctgg
    13141 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaaaaaac
    13201 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    13261 ctatgttcac cgtgatcttc gctatggcac gtacggttgg ctggatcgcc cactggagcg
    13321 aaatgcacag tgacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    13381 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagccg ttcactttcc
    13441 ggacccgcct ttaatagagc gggttctttt tatatggctg aacataaaaa attgtaaaaa
    13501 ttaaaccaga tgcaaatacc cattaataat ggtgaaaaaa tatttcaccg gaaacactgg
    13561 tgattgattt gggtaaattt aacttactgg atattcgccg ccacacaatg tcgaaaacat
    13621 tcagtatcaa gacaacaaaa tcgcaaaatg accagtgtac cgacggattt aaggtcagct
    13681 cctcgtttta taccgaaaaa acgttggttg aagaagataa agcattgttg attggtaatg
    13741 gtctgaagct acgtatactg gatgaaaacg cctcgcctta tacattcaat aaatatgctt
    13801 aatacgccga tttcaccagt gatatgctga tctacgaaaa aacctatacg gctgaattat
    13861 cgtccacacc tggcactccc atcgcggtgg gtcccttcga tacagtggta ctttttaaga
    13921 ttaactataa ctgatttaac acgacgccct tttcagccgg aaagggcaac gtattcagat
    13981 tattgctgat tttccccttt tccttgatta ccgttatttt ttccagaccc agctttcttt
    14041 atgctgtatc gatacaccaa agataaaata atgaggaagc gaagaatgcc tgtattgcag
    14101 tggggaatgt tatgtgttct gtcacttctc ctttctattg gcttcctcgc tttacatctc
    14161 ccggcggcac tcctgcttgg cccgatgatc gccgggatca tcttcagtat gcgcggagtc
    14221 actctgcaac ttccccgctc cgcttttctc gctgcccaag ccattcttgg ctgcatgatt
    14281 gcacaaaacc tcactggttc gattctcacc acgctggccg tcaactggcc gatcgtgctt
    14341 gcgattttac tggtgacgct gctttccagc gccatcgtgg gttggttatt ggtgcgctat
    14401 agctcactgc ctggaaatac cggggcctgg ggttcttctc ctggcggcgc ggcggcaatg
    14461 gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat gcaatatctg
    14521 cgagtgctgt tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct gggcgataac
    14581 gctgaagcgg ttaaccagca gatcgtctgg ttcccgccag tgagcattaa tctcctgctt
    14641 acgattttgc tcgcggtcgt tgccggtacg gcgggatgct tgctacgtct tccttccggc
    14701 acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcacgatt
    14761 gaactcccgg aatggctgct ggcgatggca tatatggcaa ttggctggcg gattggtctt
    14821 ggtttcgata agcaaatatt actgcgggca ttgcgcccac tgccacaaat cctgctgtcg
    14881 atttttgctc tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcat
    14941 attgatttta tgaccgccta cctcgccacc agccccggcg ggcttgatac cgtggcggtc
    15001 atcgccgcag ggagcaatgc cgatatggcg ctcatcatgg cgatgcaaac tctgcgcctg
    15061 ttcagtattt tgctgacggg gcctgccatc gcacggttta tttcaaccta tgcgccgaag
    15121 cgttcggcct agtgctggca gcctgggcac cagtaaaacg ggcgcgatga cagcgtggtt
    15181 ttctcaatga tgccgccaca gcgttcgcaa ggttcgccat cacgatgaaa aaccttaaag
    15241 cgaaacagcg ccccatgata tttgttttca tccacctgcc cccgcgtagc gtaagaaagt
    15301 cgcggaatat ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc
    15361 gctttatgat ttccggtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca
    15421 agtcctgcca gaaacgcctg atcgagcagt aatccagcaa actggcggtt acgaaagcgc
    15481 ggcgacaata atcgttcctt taccacctcc ggcgtcagat tcggatccag cacatcggga
    15541 ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gagtcaacat ctcaatatcc
    15601 gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg cagtactcgc
    15661 gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg
    15721 ctgtagagcg ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca
    15781 tgggtaacgt attgaccgat aagtcgtgat tgataagatt ttaactgcgg gaaggcaaac
    15841 cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg
    15901 atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaag cggggttatg
    15961 ctgcgacaac aatccccttt tcagcaaatg tagctcgtaa tcggcgggcg aacgccagtg
    16021 cgtgctcgcc atcgccatgt aggcagaccg tttgcgccgt gaccattgcc cattcgccgg
    16081 tgatactttt gactctgccg tgttgcacca tctccaacgt ttgcgccagc gcctgttctt
    16141 cgttttcaat caacgcgcct ggctggcttc gcggcaccag cgaaccgtct gcctgataac
    16201 cacgatcggc aaacacttcc tcgcgcgtta ccaggccata tcgttcgcct gcacgaatca
    16261 gctcgcttcc tgccagcccg acgagaatca atgccggatc gcaagcgtat accgctctgg
    16321 cgatggcgtc tgccagttgt gcctctttcg ccgcctggtt gtataacatg ccgtgcggtt
    16381 tgacatgaca cattacgccg ccttgcgcac gggtaatcgc tgccagcgcg ccaatttgat
    16441 acagcgtctg ggcgaaaaca gtttctggcg gcagctgcat ggcgctgcga ccaaaatttt
    16501 ccctgtcggg aaaactcggg tgcgcgccaa tcgcgacacc gttttttatt gcttcacgca
    16561 cgcaagcctg catggtttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg
    16621 aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg gcgttcaggt
    16681 caattttcat tgtgcagccg ccatgctaat tgttcgaaat aacgttgttg atcttgccgt
    16741 gctttcagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt
    16801 tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgcgggta accaccggtg
    16861 gtttgcgcat cgttcatcaa cacaatgggc tgcccgttat gcggcacctg caccacacct
    16921 ggtaacaaac cgtgagataa cagttcgcga tcggtggtgc gttttaaaat ttgcccctgt
    16981 aagcgatagc ccatccggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca
    17041 tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg
    17101 ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcgc tttgccaatc
    17161 gggagtcggt caccatccct cagtaaacgg ccttccagcc cgccaatccc caccttgaga
    17221 tcggtgctgc atgatcccat taccggcgga acatcaatac cacccgcgac cgccagataa
    17281 ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgctct cattggcaat
    17341 cgccagccgg tccagacggc attatcatcc agccgtgctt cacagcctgc gccagtcagg
    17401 gcaaaccaac catcgctttc aaattcaacc gttaactgac cgagcgtgat ctcaagtgcg
    17461 ggcgcgttgg catcattgcc caccagcagg ttagcaatac gtagcgcagg catatccagt
    17521 gcgccacagt ggctgatacc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg
    17581 gtatacatgc ccgcacgaat aatcttcagc atactccctc cttctggggt acaaagcgca
    17641 cgctgtctcc cggacgtaat aagatgggtt cgtcacgctc cggatcaaac aggctgagtg
    17701 aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca ccggtctgcg
    17761 gcccgccgat cccgacagaa cctgccggaa cgtgcaagcg cggttcagcg cgccgtggcg
    17821 tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc
    17881 agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc aatccacaat
    17941 gcgccgcgac caccgccaaa tccggtccgg ctgcgccacc gtaaaccacc ggaatttcaa
    18001 taaagcgaga ctccggctcc agcgcctcac tctcctccca ccagcgttgc aaacgctcta
    18061 tggcatccag cgccagcgac tcaggattac gcaaaatcac tgtgatattg ttcatgccag
    18121 gaatggcttc aaccacattc ggcatatcca ccagacgctg tgccagtcgc cagatccgtt
    18181 tctggctggc cagcgtcacc ggcggttcca gttccagtac taccgccgtt tcacctatca
    18241 gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga
    18301 atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga
    18361 ataccaccgc gttcagtggc atggtgaccc gcagcataaa aatgcaatcc ctgctcgcgg
    18421 gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac gccaaaacgc
    18481 gcggcgctat cgataaaact ttgcccgcct cccgtgcacc aggcgacgcg ctgaaccagc
    18541 tcagacccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggag
    18601 gccagttcca gtcccggcac aggcatggtc agttcgcccc acggcaccaa cggctcaatt
    18661 tcgcccatga cggtgatgcc aagtaatgct gccagttgtg cgttattgcc cagttcagga
    18721 tgggaatcca gcggcagatg ccagccatac aggttgatat cgttagccag caaggttttt
    18781 aaacgacggc gtttcatgcc gcgaatcacc ggagattcac ctttccagaa ataaccgtga
    18841 tgcacgatga ccgcgtcgac ctccaggcgt accgcttcat caagcagtgc ctggctggcg
    18901 gtaacaccgg taacgatttt ttgcaccgtc tctttgcctt ccacctgcaa accattcggc
    18961 gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg
    19021 tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg
    19081 cctgttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg
    19141 caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt
    19201 gcataaataa tcagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc
    19261 aactattacg tgacgttgca gtcaattgtg gaattattat gaataaacac gcatcacaac
    19321 cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca
    19381 tgcgtgccct gctgatcctc tatctcacca atcaactaaa atatagcgat aatcacgcct
    19441 acgagttatt tagcgcctac tgttcgctgg tatatgtcac gccaatcctc ggtggctttt
    19501 tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgttg ttgatggcga
    19561 tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg
    19621 cgattatcgt ctgcggctat ggtctgttta aatccaacgt cagttgcctg ctcggcgagc
    19681 tgtatgagcc aaccgatcca cgtcgtgatg gcggtttctc gctgatgtat gcggcgggta
    19741 acgtggggtc tattatcgca cctatcgcct gtggtttcgc ccaggaagag tacagttggg
    19801 cgatgggctt tggcctggcg gcggtgggta tgatcgcggg tctggtcatt ttcttatgtg
    19861 gcaatcgtca tttcacacat acccgcggcg ttaacaaaaa ggtactgcgt gcgacaaact
    19921 ttctcctgcc caactgggga tggctactgg ttctgctggt ggcaacgcct gcgctgatta
    19981 ccgtgttgtt ctggaaagag tggtcggtat acgccttgat tgtcgcgacg atcattggcc
    20041 ttggggttct ggcaaaaatt tatcgcaaag cagaaaacca aaaacagcgt aaggagctgg
    20101 ggctgattgt taccctcacc ttcttcagca tgttgttctg ggcattcgca caacaggggg
    20161 gtagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatgttt ggttataccg
    20221 ttccgactgc tatgttccag tcgattaatg ccttcgcagt tatgctgtgc ggtgtgttcc
    20281 tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc tggggaaaat
    20341 ttgctcttgg ccttggcctg atgagcgccg gattctgcat tctgacctta agcgcccgct
    20401 ggtccgcaat gtatggtcac tcttctctgc cactgatggt gttaggcctg gcggtgatgg
    20461 gctttgcgga actgtttatc gatccggttg ctatgtcgca gattacgcgc attgaaatcc
    20521 ccggcgtgac cggcgtatta accggcattt atatgctgct ttccggcgcg attgcgaact
    20581 atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct tccggggcga
    20641 tcgactactc catcaatgca tatattgaag tatttgatca aattacctgg ggcgcactgg
    20701 cgtgtgtagg actggtactg atgatttggc tgtatcaggc gcttaagttc agaaaccgcg
    20761 cgctggcgct ggagtcttaa taagccagca ccatgcctga taaaacgctc gctgcgtttt
    20821 atcaggctcc tttcctcgct tcttcgtacg ctgccagcgt tcgcagcctt gcctctttgt
    20881 gatcgactat cggttgcgga taatccaacg tcacacctgt tttctctgcc cacttccacg
    20941 gctcatgcac cgctttcccc ggtacatcgc gcagctccgg tagccactgg cggataaatt
    21001 cgccttcgcg gtcaaatttc tcgccctgag ttgtcgggtt gaaaatacga aaatacggcg
    21061 ctgcatcagt acccgtagaa gcagcccact gccagccacc gttattagct gccaaatcac
    21121 catcaatcaa ctgcgacatg aaatatcgct cgccttcgcg ccagtcgatc aacaaatctt
    21181 tcaccagaaa actggctgta atcatccgta gccggttatg catccagcca gtgctgttaa
    21241 gctgacgcat ggcagcatcg acaatcgggt atcccgtttt gccctcctgc caggccttta
    21301 aatgggcggg attgctttgc cactgtacac gatccgtcca ggcaataaac ggacgatgtt
    21361 tacacagcga aggataatac gtcatcaaat ggcggtagaa ctcgcgccat atcagctcat
    21421 taagccagac gctccccggt ccgccttcca gcacctgcgg ttgttccgcc aacaggcgat
    21481 gtaaacactg gcgaggcgat aacccgcccg ttgccaggct ggcggacaaa cgactggtgc
    21541 cttccactgc cggaaaatct cgttgttgct catactctcc ggcaccgttc tggcaaaact
    21601 ggcgtaattg cgcaatcgcc gctttttctt ccaccgggaa atgcgcagta tcgaaagact
    21661 gacgaggata attcagcgta atggatggcg cgggctctat cgatccgcta ctacgaactt
    21721 ttggtgcagc gacgcactcc ggcatccctt cccgcagccg tttcaaccat gcgtttttaa
    21781 aaggcgtaaa gactttgtac atctcatgat tgccagtcat caccgccccc ggcggcagga
    21841 tcacgctgtc atcaaatcct tcacacacca cgttacgcag cgtcctctca gcctgcacat
    21901 cccgcgcccg ctcattcacc tcatactgat agttataaaa cagatgggta atgcgatttt
    21961 ccgcgcacac ctgtttaact gtttcgacac tggcagcaaa gtcatccact tcgcggaaca
    22021 ataaaggaat gcctttttcc gccagcgcgt tttgcagcgc attaagttgt gtattgataa
    22081 gttcagcctg acgcggcgac acattatgcg ccgcccactg gcgtggtgta gcgatataca
    22141 acgccagcac gcgcgcagac gaattgcggc aggcggcagc cagcgcgaga ttatcgtgct
    22201 gacgtaaatc ctggcgaaac cagaccagat gggtagtcat aaaactcctg catggcgcaa
    22261 gttaatcaca cgcggccaaa gttcgaaata acgctgccct gaaagccagg cgtcaggata
    22321 aagcgccata tagtgtttga tacgcatcag cggcgcaaga agcggttgct cgccacggcg
    22381 ataactgtcg ataagcgccg ccagctgctg gcgttctgtg gaatcaatat gtgggcgaaa
    22441 atatccctga gcgtgcatca aaacgttagt atgatcacgc cggttggcag ggtgagaaag
    22501 caaaacaatt acgcgttggc ggtactggtt gtaatagtca tccagacttt gccaccggtg
    22561 aatatcagcc acaaacgggc caagtttgcg gtactccggc tgagaatgtg ccagaaagac
    22621 gagtttatag cggctgtgat aatcgagaag cgccccgcgc gttagtcctc gatctttgag
    22681 attgtgtaac tcatgcaaag caaaaatgcg gataatggtg ctgtcatcaa aaagttgttg
    22741 attcattact gctcctgaac ggtttacagg agtaagtgta gacgccgaaa aaagaaaacc
    22801 gccagcgttt ccaccggcgg ttttctttaa acgtcatgtt tggcaatcag taaaaatcac
    22861 cggttgcctt ttcggcctga tccatccaca ccggtttatc gctggttttc gaccagacgc
    22921 ggtgcaggta gctataaaag cgggcgcggt ctttccagaa aagcatgaaa ggcagcgcca
    22981 gaacgcccgc tgcaaccgca caagtacggc gtaaaaagat aagccatgca ggatattctc
    23041 tgtagagttc catacttttc tccccctttt gtatctaccc ggtgaatggc accggaaaaa
    23101 tgaatttgtt tatctgatga aaatagtacc gccttttgtg taattttact actcatccga
    23161 ccacttattt ttgcttattg atggtttatt tacattcata ccgtaattaa gttacacaaa
    23221 ggttaaatta atactaaaca ttagttaaat catggctttt gccattttta tacttttttt
    23281 acaccacgcc cgcagatttt tgcgaaatct ttgcagccag aactctaccc ttccagtatc
    23341 acttttaggc cactggaggt gcactgtgag tgcaggcgtg atagccggcg tgttgctggt
    23401 gtttttatta ctgggttatc tggtttatgc cctgatcaat gcggaggcgt tctgatggct
    23461 gcgcaagggt tcttactgat tgccacgttt ttactggtat taatggtgct ggcgcgtccc
    23521 ttaggtagcg ggctggcgcg gctgattaat gatattcctc ttcctggtac gacgggcgtt
    23581 gaacgcgtac tctttagcgc gcttggcgtc tctgaccgcg agatgaactg gaagcagtat
    23641 ctctcagcca ttcttggcct gaatatactg gggctggcgg tgctgttttt tatgttgctc
    23701 ggtcagcact atctgccgct taatccacag cagttgccgg ggctgtcgtg ggatctggcg
    23761 ctcaataccg ccgtcagctt tgtcacgaat accaactggc aatcttatag cggtgaaacc
    23821 acgttgagct atttcagcca gatggcgggc ttaacggtgc aaaactttct ttctgccgcc
    23881 agcgggattg cggtgatttt tgcgcttatt cgcgcgttta cccgccagag catgaacacg
    23941 ctgggtaatg cgtgggtaga cctgttgcgg attacgttat gggtgttaac ccccgtagca
    24001 ttgctgatcg cactgttttt tattcaacaa ggtgcgctgc aaaacttttt gccttatcag
    24061 gctgtgacga ccattgaagg agcgcaacag ctgttaccaa tggggccagt agcttctcag
    24121 gaagcgatca aaatgctcgg aactaacggc ggtggctttt ttaatgccaa ctcgtcgcat
    24181 ccgtttgaaa acccaaccgc actgaccaac ttcgtgcaaa tgctggcgat tttcttgatc
    24241 ccaacagcgc tgtgctttgc ctttggtgaa gtggcgggcg atcgtcgcca ggggcgcatg
    24301 ttgctgtggg cgatgtcggt gatttttgtc atctgcgtag gcgtagtgat gtgggcagaa
    24361 gttcagggta atcctcatct gctggcgctc ggcgcggaca gcagcatcaa tatggaaggt
    24421 aaagagagcc gcttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggct
    24481 tcctgcggcg cggtgattgc gatgcatgat tcgtataccg ctcttggtgg catggtgccg
    24541 atgtggttga tgcaaattgg tgaagtggta ttcggcggcg tcggttccgg cctttacggc
    24601 atgatgctgt ttgtcctgct ggcggtgttt attgccgggc tgatgattgg tcgtacaccg
    24661 gaatatctgg gtaaaaaaat cgacgtacgc gagatgaaac tgactgcact ggcgattctg
    24721 gtcaccccga cgctggtgct gatgggcgcg gcgctggcaa tgatgaccga cgccggacgt
    24781 agcgccatgc tcaaccctgg cccgcatggt tttagcgaag tactgtacgc cgtgtcgtcc
    24841 gccgctaaca acaacggcag cgcctttgcc ggattaagcg ccaactctcc gttctggaac
    24901 tgtttactgg cgttgtgcat gtttgtcggg cgcttcgggg tgattatccc ggtgatggca
    24961 attgccggtt cgctggtgag taaaaagagc caacccgcca gctccggcac attgccaacg
    25021 cacggcccgc tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc
    25081 tttatccctg ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg
    25141 agcactgaat atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc
    25201 gctgaaagaa gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt
    25261 tatcgtctgg atcggcagtc tgctgaccac ctgtattagc atcgcgatgg caagcggtgt
    25321 gatgcctggc aatgcgctgt ttagcgcggc aattagcggc tggctgtggg tcaccgtact
    25381 gttcgctaat ttcgccgagg cgttggcaga aggccgcagt aaagcgcagg ccaacagtct
    25441 gaaaggggtg aaaaaaaccg cttttgctcg caaactgcgc gaaccgaaat atggcgctgc
    25501 ggcggacaaa gttcctgctg atcaactgcg taaaggtgac atcgtgctgg tagaagctgg
    25561 cgatattatc ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc
    25621 catcaccggg gaatcggctc cggtgatccg tgaatccggc ggtgattttg cctccgtcac
    25681 tggcggcacg cgtattcttt ctgactggct ggtgattgag tgcagcgtta accccggcga
    25741 gacatttctg gatcggatga tcgcgatggt ggaaggtgcg cagcgacgta aaacgccaaa
    25801 cgagattgcc ctgaccattc tcctgattgc cctgactatc gtctttttac tggcaaccgc
    25861 cacgctgtgg ccgttttccg cgtggggcgg taatgcagtc agcgtaacgg tactggtggc
    25921 actgctggtc tgtctgatcc caaccactat tggcggcctg ttgtcagcga ttggcgtcgc
    25981 cgggatgagt cggatgctgg gcgcgaatgt gattgccacc agcggacgtg cagttgaagc
    26041 ggcaggtgac gttgacgttc tgctactgga taaaaccggc accatcaccc tcggtaaccg
    26101 tcaggcgtca gagtttatcc ccgcacaagg cgtggaggaa aaagcgctgg ccgacgccgc
    26161 acaactggct tcgctggctg atgaaacgcc ggaaggccgc agtattgtga tcctcgccaa
    26221 gcagcgtttt aacctgcgcg agcgcgatgt gcagtcgctc catgccacct ttgtaccttt
    26281 tactgcacaa agccggatga gcgggatcaa catcgacaac cgcatgatcc gtaaaggttc
    26341 tgtcgatgcc attcgccgcc atgttgaggc taacggcggt cacttccctg ccgatgttga
    26401 tcaaaaagtc gatcaggttg cgcgtcaggg agccacgccg ctggtggtgg tggaaggttc
    26461 tcgtgtgctg ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt
    26521 cgcccagctg cgtaaaatgg gcattaaaac ggtgatgatt accggcgata accgcctgac
    26581 tgccgccgcg atagctgcgg aagcgggtgt cgatgatttt ctcgccgaag caacaccgga
    26641 ggccaagctg gcattgattc gtcagtatca ggcagaaggc cgtttagtgg cgatgaccgg
    26701 cgacggcacc aacgatgctc cggcgctggc gcaggcagat gtcgcggtgg cgatgaactc
    26761 cggtactcag gcggcgaaag aagcgggcaa tatggtggat ctcgactcta acccgaccaa
    26821 gttgatcgag gtggtacata ttggcaaaca gatgctgatg acccgtggct cgctgaccac
    26881 cttcagcatt gccaacgatg tggcgaaata cttcgccatt attccggcgg cgttcgcggc
    26941 aacgtatccg cagttaaatg cgctgaacat catgcgcctg cattcgcccg actccgccat
    27001 tctcagtgcg gtcattttca acgccttgat tatcgtcttt ttgattcccc tggcgttaaa
    27061 aggcgtgagt tataaaccgc ttaccgcttc tgccatgttg cgccgtaact tatggattta
    27121 cggtctgggt gggctgctgg tgccgtttat tggtatcaaa gtcattgatt tactgctaac
    27181 cgtttgcggt ctggtgtgag gtttaccatg cgtggattac gtccggcatt atcgacattt
    27241 ctctttctgt tattgattac tggcggcgtt tacccgctgc tgaccaccgc actggggcaa
    27301 tggtggtttc cctggcaggc caacggttca ttgattcgtg aaggtgatac ggtgcgcggt
    27361 tcggcattaa tcgggcaaaa ttttaccagc aacggctatt ttcatggtcg cccgtcggca
    27421 acagcagaaa tgccctataa tccacaggct tctggcggga gcaacctggc ggtcagtaac
    27481 ccggagctgg ataaacagat agccgcacgc gttgctgcat tacgggctgc taacccggat
    27541 gccagcacga acgttccggt tgaactggtg acggcgtcag caagcgggct ggacaataac
    27601 atcaccccgc aagcggcggc ctggcagatc ccacgcgtgg cgaaagcgcg taatctcagc
    27661 gttgaacagc tcacgcaact gatcgcaaaa tacagccaac aaccgctggt gaaatatatc
    27721 ggccagccgg tcgtcaacat tgtcgaactc aatctggcac tggataagct tgatgaataa
    27781 tgaaccctta cgtcccgacc ccgatcgtct gctggaacaa gctgccgcgc cgcatcgggg
    27841 gaagctgaaa gtattcttcg gtgcctgcgc aggcgtcggg aagacctggg cgatgctggc
    27901 ggaagcccag cgactgcggg cgcaagggct ggatattgtg gttggcgtgg tagaaaccca
    27961 cggtcggaaa gataccgccg caatgctgaa aggtctggct gttctgccgt taaaacgcca
    28021 ggcgtaccgt gggcggcaca tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc
    28081 ggcactgatc ttaatggacg aactggcgca cagtaatgcg ccaggttctc gtcatcctaa
    28141 acgctggcag gacatcgaag agctgctgga agcaggcatt gatgttttca ctaccgtcaa
    28201 cgttcagcat ctggaaagcc tgaacgatgt ggtcagcggc gtcaccggaa ttcaggtacg
    28261 ggagaccgtg cccgatcctt tttttgatgc cgccgacgac gtagtactgg tggacttgcc
    28321 cccggacgat ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg ggcaagcgga
    28381 gcgcgccatt gaacattttt ttcgcaaagg caatctgatc gccctgcgcg aactggcact
    28441 gcgccgtact gccgatcgcg tagatgagca aatgcgcgcc tggcgggggc atcctggcga
    28501 agagaaagtc tggcacacac gcgacgcgat ccttttatgc atcgggcata acaccggcag
    28561 tgagaaactg gtccgcgcgg cggcgcggtt ggcctcgcgc ctgggcagtg tctggcacgc
    28621 ggtgtatgtt gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc gggcaattct
    28681 cagcgcctta cgtctggcgc aggagctggg cgcggagacg gcaacacttt ctgatccagc
    28741 ggaagagaaa gcggtagtgc gttatgcccg tgaacataat ctcggcaaga ttattctcgg
    28801 tcgcccggct tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac tggcgcgtat
    28861 cgcccccgat ctcgatcagg tgctggtcgc acttgatgcc cccctcgccc gcacgcttaa
    28921 taacgcgccg gatagccgct cttttaaaga caagtggcgg gtacagattc agggatgcgt
    28981 ggttgccgcc gcgttatgcg ccgttatcac cttaattgcc atgcagtggc tgatggcgtt
    29041 tgatgccgcc aacctggtga tgctgtatct gcttggcgtc gtggtggtgg cactatttta
    29101 cggacgctgg ccttcggtgg ttgccaccgt cattaatgtg gtgagtttcg atctcttttt
    29161 tatcgcccca cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga ccttcgcggt
    29221 gatgttaacc gtcgggctgg tgatcgggaa ccttactgct ggcgtgcgtt atcaggcgcg
    29281 ggtagcccgt taccgcgaac aacgcacacg gcacttatat gaaatgtcca aagctctggc
    29341 tgtgggccgt agttcgcagg atatcgccgc caccagcgaa cagtttattg cctccacgtt
    29401 tcatgcccgt agtcaggtgt tgctgcctga tgacaacggt aaattgcagc ctttaacaca
    29461 tccgcaagga atgacgccgt gggacgatgc catcgcgcag tggagttttg ataaaggcct
    29521 gcctgcgggc gcgggcaccg acacgttacc cggtgtaccg taccagattt tgccgctaaa
    29581 aagcggcgag aaaacctacg gactggtggt ggtggaaccg gggaatctgc gccagttgat
    29641 gatcccggaa cagcagcgcc tgctggagac gtttacgctg ttagtcgcca atgcccttga
    29701 gcgactgaca ctgaccgcca gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat
    29761 ccgcaacgcc ctgctggcgg cgctttcgca tgatttacgc acgccgctta cggtgctgtt
    29821 cggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcgcccc acgcccgcca
    29881 ggccagcgag attcgtcagc atgtgctgaa cacgacccga ctggtgaata atctgctcga
    29941 tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa cgctggaaga
    30001 agtggtcggc agcgcgctgc aaatgctgga accgggttta tcgtcgccca tcaatctttc
    30061 tctgccagag ccgctgacct taatccacgt tgacgggcca ctctttgaac gggtgctgat
    30121 taacctgctg gagaacgcgg tgaaatatgc gggggcgcag gccgaaattg gtatcaatgc
    30181 ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg gtcttccacc
    30241 aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt cggcggtacc
    30301 gggtgtaggg cttgggttgg caatttgtcg ggcgatagtg gatgtacacg gcggcaccat
    30361 taccgcattc aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc cccagcaaac
    30421 agcccctgaa cttgaagaat ttcatgagga tatgtgacaa acgttctgat tgttgaagat
    30481 gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacggtat gcgcgtcttt
    30541 gaggccgaaa cgctgcaacg cggcttgctg gaagcggcaa cccgtaagcc agatttgatt
    30601 attctcgatc tcggcctgcc cgacggtgat gggattgaat ttatccgcga cctgcgccag
    30661 tggagcgcgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga taaaatcgcc
    30721 gcgctggatg ccggagcgga tgattatctg agtaagccgt ttggcattgg cgaattgcag
    30781 gcccgtctgc gcgtcgcatt acgccgccac tccgccacca ccacgcccga tccgctggtg
    30841 aaattttctg atgttaccgt cgatttagcc gcccgcgtta tccaccgcgg cgaggaagag
    30901 gtgcatctca caccgattga gttccgcctg ctggccgtgc tgctcaacaa cgccggaaaa
    30961 gtgctcaccc agcgccagtt actcaatcag gtgtggggac caaacgcggt cgaacacagc
    31021 cactatttgc gtatttatat ggggcatctg cgacaaaaac tggagcaaga tcccgcccgc
    31081 ccccgccatt ttattactga aaccggt
//
LOCUS       DAZFRE010000058        30756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFRE010000058 DAZFRE010000000
VERSION     DAZFRE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.058"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            18..554
                     /locus_tag="ACHKUI_002963"
     CDS             18..554
                     /locus_tag="ACHKUI_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599124.1"
                     /translation="MTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDAYPDVQV
                     SWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEMKNDIRR
                     FLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENAQFLEWV
                     WTQLMEATKFAYEGSVNG"
     gene            547..789
                     /locus_tag="ACHKUI_002964"
     CDS             547..789
                     /locus_tag="ACHKUI_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599125.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            810..956
                     /locus_tag="ACHKUI_002965"
     CDS             810..956
                     /locus_tag="ACHKUI_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599126.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            964..1218
                     /gene="xisR"
                     /locus_tag="ACHKUI_002966"
     CDS             964..1218
                     /gene="xisR"
                     /locus_tag="ACHKUI_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6599127.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            1252..2541
                     /locus_tag="ACHKUI_002967"
     CDS             1252..2541
                     /locus_tag="ACHKUI_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599128.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITTN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPRKIQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            2586..3236
                     /gene="rcsB"
                     /locus_tag="ACHKUI_002968"
     CDS             2586..3236
                     /gene="rcsB"
                     /locus_tag="ACHKUI_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6599129.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3436..6285)
                     /gene="rcsC"
                     /locus_tag="ACHKUI_002969"
     CDS             complement(3436..6285)
                     /gene="rcsC"
                     /locus_tag="ACHKUI_002969"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6599130.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            6452..8278
                     /gene="atoS"
                     /locus_tag="ACHKUI_002970"
     CDS             6452..8278
                     /gene="atoS"
                     /locus_tag="ACHKUI_002970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO6599131.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8275..9660
                     /gene="atoC"
                     /locus_tag="ACHKUI_002971"
     CDS             8275..9660
                     /gene="atoC"
                     /locus_tag="ACHKUI_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO6599132.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTTPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            9856..10518
                     /gene="atoD"
                     /locus_tag="ACHKUI_002972"
     CDS             9856..10518
                     /gene="atoD"
                     /locus_tag="ACHKUI_002972"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO6599133.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10518..11168
                     /gene="atoA"
                     /locus_tag="ACHKUI_002973"
     CDS             10518..11168
                     /gene="atoA"
                     /locus_tag="ACHKUI_002973"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO6599134.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11165..12487
                     /gene="atoE"
                     /locus_tag="ACHKUI_002974"
     CDS             11165..12487
                     /gene="atoE"
                     /locus_tag="ACHKUI_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO6599135.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12518..13702
                     /gene="atoB"
                     /locus_tag="ACHKUI_002975"
     CDS             12518..13702
                     /gene="atoB"
                     /locus_tag="ACHKUI_002975"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO6599136.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESSALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(13776..14552)
                     /gene="yfaP"
                     /locus_tag="ACHKUI_002976"
     CDS             complement(13776..14552)
                     /gene="yfaP"
                     /locus_tag="ACHKUI_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6599137.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14557..16206)
                     /gene="yfaQ"
                     /locus_tag="ACHKUI_002977"
     CDS             complement(14557..16206)
                     /gene="yfaQ"
                     /locus_tag="ACHKUI_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO6599138.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQMSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQQIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16207..20721)
                     /locus_tag="ACHKUI_002978"
     CDS             complement(16207..20721)
                     /locus_tag="ACHKUI_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO6599139.1"
                     /translation="MGTGLANADDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGELVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQKSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKSLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALTLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEVDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(20745..21368)
                     /gene="yfaT"
                     /locus_tag="ACHKUI_002979"
     CDS             complement(20745..21368)
                     /gene="yfaT"
                     /locus_tag="ACHKUI_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO6599140.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21365..23053)
                     /gene="yfaA"
                     /locus_tag="ACHKUI_002980"
     CDS             complement(21365..23053)
                     /gene="yfaA"
                     /locus_tag="ACHKUI_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6599141.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTQVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(23202..25829)
                     /gene="gyrA"
                     /locus_tag="ACHKUI_002981"
     CDS             complement(23202..25829)
                     /gene="gyrA"
                     /locus_tag="ACHKUI_002981"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6599142.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            25976..26698
                     /gene="ubiG"
                     /locus_tag="ACHKUI_002982"
     CDS             25976..26698
                     /gene="ubiG"
                     /locus_tag="ACHKUI_002982"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6599143.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(26838..30596)
                     /gene="yfaL"
                     /locus_tag="ACHKUI_002983"
     CDS             complement(26838..30596)
                     /gene="yfaL"
                     /locus_tag="ACHKUI_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6599144.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NSNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGVAGNTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVHQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         7544 a         8192 c         7504 g         7516 t
ORIGIN      
        1 gttacagggt ccccacaatg acatcgtggg aaagtcttgc agaagagcgg ggaggctctc
       61 tggataaata tatcgtcagt ccactattca ctgttcctca gataccgcca gtgagaacag
      121 aacaggatgc ttaccctgat gtgcaggttt catgggatgc aacacgtcac tatgtcaacg
      181 gctggaacgc ctgccgacag gcaatgctga cgacggcaga aaaagtactt gatgtgcgaa
      241 tccctgaata tatcggacgc tggctttccg ctgcacttca tgatcagaac tcatgcgagg
      301 aaatgaaaaa cgatatacga cgcttcctgg ctgaatacca ccatgttcca tcgaaatata
      361 acggctggcg acttgtaccg gacaagttaa ccgaacagat gaagtaccgg attgtgccac
      421 tactggcgac catcaagccc cgcagcgtcg atgaatatga ccgctcagga gaaaacgcac
      481 aatttcttga atgggtatgg acgcaactga tggaagccac gaaattcgcg tatgagggct
      541 cagtgaatgg gtgaaatggt tgatattaac gatgaactgg tccgcacagt aattaccgtt
      601 gatgacggat gcgaccatac ccagcgtttt atctggatga tgaatacacg gcgtaatatc
      661 agaaacaaag tgttccggca cgaaccaaag ccagtcaggg tgacaaagat acgcattgaa
      721 gccagaaaac gccgcaaata caggccacgg aaggcctggg ccccagcgca accggatgaa
      781 ctggcataaa gacagcagga gaataagtcg tgaatcgcga gtttgaaata tggatacgac
      841 tcagatacgg gtaccgatac gatttaacca gagatgctca cggcttctac tgccgtgaag
      901 ttgtaaagcg aatgtttgac gtgtggtgcc actgccgtgg cctgaagata gtgtgagtca
      961 ggaatggaaa caatcatcta tcagataacg cccagcaaat ggtgcacaga aagaaccctt
     1021 attgctgcaa ctggactgaa atcaggaacc attgccagag caagaaaaaa atcctggtta
     1081 ctggggaaag aataccgtca ttacgcagca gaaggagagc ccgggccttt cagtgaatgt
     1141 gtatacaaca ttgaggaaat tatgcgctgg atcgaaaacc agaaacaacc aggtgccaaa
     1201 aatgcaagtt ccggttaacc tgttaatgct cctggacgtc tgggaggttt aatgagtaac
     1261 gcatcatacc cgacaggcgt tgaaaaccat ggcggatcac tccgcatatg gtttcactat
     1321 aacggcaaac gtgtcagaga aaacctcggt gttcctgaca ccgccaaaaa ccggaagatc
     1381 gcaggtgaac ttcgcacttc cgtttgtttt gcaataagaa tggggagctt cgactacgcc
     1441 gcgcagttcc ctaattcccc taacctgaaa cattttggtc tgggaaaaaa agagataacc
     1501 attaaggcac tttcggaaaa atggcttgag cttaagaaaa tggaaataac aacaaatgcc
     1561 atggtcagat acaaatcagc tatccgaaat tgccttgact gccttggagg gaacaaactg
     1621 gcatcatcaa ttacacagga agatgttctc atttttcgta aagaattatt aacgggatat
     1681 cagttgccaa gaaaaattca gaggaagcct attaaaggtc gtagtgtacc tactgtaaat
     1741 agctatatga caattatctc cgggatgttc cgttttgcgg caaacaatgg gtatataggc
     1801 aaaaaccctt tcagtgagct tccatcactg aaaagagcga aaacacctcc cgatccacta
     1861 acaaaagatg agtttgccag actgattgat gcgtgccacc accagcaaac taaaaatttc
     1921 tggtcgctgg ctgtatatac cgggatgcgg catggcgaac tgtgtgggct ggcatgggag
     1981 gatatcgatc tggaagcggg taccttgacc gtcaaaagga attacactca gacaaaggaa
     2041 tttacactac ccaaaacaga tgcaggaaca aacagggtaa tacaactcat acagccagca
     2101 attgacgtat taaaaaatca ggcgacatta accaggcttg gtaaacaata tcagatagaa
     2161 gtaaagctgc gagagtatgg ccggacaagc actcactcat gcagctttgt tttcaatcct
     2221 cagatattag atcgcacagg taactcagga gttcattatg ccgtaacatc catcaacaga
     2281 acatgggaat ctgcattaaa acgagcaaaa attcgccata gaaaagcgta tcagtctcgg
     2341 catacttatg cttgctgggc tttaactgcc ggagcaaatc caaacttcat agccaaccag
     2401 atgggacatg cgaatgccca gatggtttac aatgtttacg gtgcatggat gaaagacaat
     2461 aaccagtccc aggtagaagt actaaacaga aagctatgta cttatgtccc acaggtgccc
     2521 caacgactaa acaaagcata gaatatattt ttaaaatcaa atagttaata cataggtaca
     2581 agtacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc ttgttcggta
     2641 ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt gaagactcta
     2701 cagcactgat caacaacttg ccgaaactgg atgcacatgt gttgattacc gatctctcca
     2761 tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag cgccatttcc
     2821 caagcctgtc gatcattgtt ctgaccatga acaacaaccc ggcgattctt agtgcggtat
     2881 tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat ctgccgaaag
     2941 ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct cgcctgttgg
     3001 aaaaaatcag tgctggtggt tatggtgaca agcgtctctc gccaaaagag agtgaagttc
     3061 tgcgcctgtt tgcggaaggc ttcctggtga ccgagatcgc taaaaagctg aaccgcagta
     3121 ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggtgtc gagaacgata
     3181 ttgctctgct gaattatctc tcttctgtga cgttaagtcc ggcagataaa gactaatcac
     3241 ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcacggat tgccggatgc
     3301 ggcgtaaacg ccttatccgt cctacgattc ccattatttc aacagattac attaaagtag
     3361 gccagataag acgcgtcagt gtcttatctg gcatttgcac ggattgccgg gtgcggcgta
     3421 aacgccttat ccgtcctacg attcccgcga tttcctgacc ctctcggcat ataccgtcag
     3481 cgtctgtttt atcacatcca gcgttactgg cttcgacagg cagctgtcca taccggactc
     3541 cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta ccggcaacgt
     3601 cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg gcatattgac
     3661 gtcgctaagc acgatatcaa tatgattctt gttaagtaca ttaagcgcat cgacgccatc
     3721 attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca gacgtcggtt
     3781 aatcggatga tcatccacga ccagaatcat catatcgtca ttatcgctga ccgctttgtc
     3841 cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat aaatacgcgc
     3901 caacaatgcc ggtagctcat gcggagcagc cacactgtgt acccactccc ctggcgcttt
     3961 ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctgc cctgccattt
     4021 tttactcact acctcgtcag tgattaacac atcttcggga gtcggttcct gcccttcgta
     4081 tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac agagcgacgc
     4141 attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt ttttctgcgg
     4201 atactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc ccggttctga
     4261 atctaccgag atgtcgccgt ccatcatgct gatcagtttt tcacaaatcg ccagacccag
     4321 accagtcccc tggaagttac gctgtacgcc cgttccgacc tggaagaagg gatcaaacaa
     4381 gcgcaccact tctttcgccg gtatccccac gccggtatcg cgaacacgga tagagagata
     4441 atcgccatca gcgcgaacat gcaaaactat acagccggta tcggtgaatt ttatggcgtt
     4501 actcagcagg ttagagatga cctgttgtaa acgcatcggg tcgccattta aggccactgg
     4561 cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca gcggtaaata
     4621 gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa
     4681 ctgttctgat tcaattttcg agaaatcgag aatatcgctg ataattttca acaacaggct
     4741 ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact ctttggtttg
     4801 caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat gactgacggt
     4861 ggccaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca tctcctgcaa
     4921 cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg ccacgttttc
     4981 attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc tggtcaggac
     5041 atcaacaaaa ttgacctgct gcccacagat aatttgtgtc agccgttggc ggtcctcatg
     5101 cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga cgccatcagc
     5161 ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat taaactgctc
     5221 atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgcc gctcgtacat
     5281 ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg cgttaaggat
     5341 caacatgcga atgcgttcca gcaccttatc aactggcact gaataaacga tgcttagcga
     5401 tgagggtggc agatttttct tcagcaccag ttcgcggaac ccttccgtat agccaaacca
     5461 ggagcgttcc tgcatccagc gaggatcgcc cttaattttg ctttctggtc cggtaagcga
     5521 aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg taccaggtaa
     5581 gaaaaagttc tccatccgga tggtttgctc gacacccaaa agcgcctgca accggttcgc
     5641 cagataaact ggcgtcaagg cgtaaaaata cccgacgcca gggcgcggac cttcgctgat
     5701 ccagtagagg ttactgccac tatcatcttg tggtgcattt cgatatttat taatgcgttc
     5761 atgcaaagct tttaacgcgg tatcgcgttc cactggcata tcacgcagac cgaaattagc
     5821 catgcagagg ttatcgctgc cgattaaaaa tacccggttt agatcgtaag ccgcagaaaa
     5881 attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc gccaggtgtt
     5941 actcattgcg gaacaatcag agtcagcaaa tagcggttca aacgcaggca catccgtctg
     6001 cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat tttcagctat
     6061 gtacttcagc tctttcatca catcagaggt gcgttgaata aagcgctgag cctgatcgga
     6121 actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat taacgatgta
     6181 aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc tgaacatgta
     6241 gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg cgaagctccg
     6301 cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc
     6361 tctcttctct ggaatatgat acaccgccga gaaatcatca ccttaacctc tgataatcgt
     6421 catataccgg acaagactag tggatttcag catgcattat atgaagtgga tttatccacg
     6481 ccgcttacgc aatcaaatga tcctgatggc aatcctgatg gtcattgtcc caacgcttac
     6541 tattggttat atcgtagaaa cggaaggacg ttcagcagtc ttatctgaaa aagagaaaaa
     6601 actttctgcc gtggtcaacc tgcttaatca ggcgctaggc aatcgctacg atctctacat
     6661 cgacttaccg cgtgaggagc gtatccgcgc attaaatgca gaacttgccc ccattaccga
     6721 aaatatcact cacgccttcc ctggcatcgg cgctggttat tacaacaaaa cgctggatgc
     6781 gataatcacc tacgcgcctt cagcgctata tcagaataat gtgggcgtta ccattgccgc
     6841 agatcaccct ggtcgcgaag tcatgcgtac aaatacccct ttggtttatt caggcaggca
     6901 ggtgcgcggc gatattttga attcaatgat tcccattgag cgtaatggtg aaatcctcgg
     6961 ctatatctgg gccaatgaat taaccgaaga tattcgccgc caggcctgga aaatggatgt
     7021 gaggattatc attgtgctca ccgccggttt gctgataagc ctgctgttga ttgtcctttt
     7081 ctcccgtcgc ctgagcgcca atatagatat catcaccgat ggcctctcga ctctggctca
     7141 aaatattccc actcgattac cacaattgcc cggtgaaatg gggcaaatca gtcagagtgt
     7201 taacaacctc gcccaggcac tgcgtgaaac gcgaacactt aacgatctga ttattgaaaa
     7261 cgctgccgat ggcgtcattg ccattgaccg ccagggtgat gtaaccacca tgaacccagc
     7321 agcagaagtc atcactggct atcaacgtca tgaactggta gggcagcctt actccatgtt
     7381 gttcgacaat actcagttct acagtcccgt actggatacg ctggaacatg gcaccgaaca
     7441 tgtagcactg gagatcagtt ttccaggccg tgaccgcacc attgaactca gtgtcacgac
     7501 cagtcgtatt cataacacgc acggtgaaat gataggtgct ttggtgattt tctctgattt
     7561 aactgcccgc aaagaaaccc agcgccgcat ggcgcaagca gaacgcctcg ccacactggg
     7621 tgagctgatg gctggcgtgg cgcatgaagt acgtaatccg ttaacggcta ttcgtggtta
     7681 tgtacagatc ttgcgccaac aaacccgtga cccaatacat caggaatatc tgtccgtagt
     7741 actcaaagaa atcgattcaa ttaacaaagt tattcagcaa ttgctcgaat tttcacgtcc
     7801 acgccacagt caatggcaac aagtcagcct caatgcattg gttgaagaaa ctctggtact
     7861 ggtacaaacc gccggcgtac aagcgcgggt cgacttcata agcgaactgg ataatgaatt
     7921 aagcccgatt aacgccgatc gtgaactgct caaacaggta ctactgaata tcctgatcaa
     7981 tgccgtccag gctatcagtg cacgagggaa aattcgcatt cgaacctggc agtatagcga
     8041 ctcgcaacag gccatttcga tagaggacaa cggcagtggc attgatctct cgctgcaaaa
     8101 aaagatcttc gatccctttt tcactaccaa agcctcagga accgggcttg gtctggcgtt
     8161 aagtcaacgc atcattaatg cccatcaggg tgatattcgc gtcgccagtt tgccgggcta
     8221 cggcgcaacc ttcacgctta ttttaccgat caacccgcag ggaaatcaga ctgtatgact
     8281 gctattaatc gcatccttat tgtggatgat gaagataatg ttcgccgtat gctgagcacc
     8341 gcttttgcac tacaaggatt cgaaacacat tgtgcgaaca acgggcgcac agcattacac
     8401 ctgtttgccg atattcaccc tgacgtggtg ttgatggata tccgtatgcc agagatggac
     8461 ggcatcaagg cactaaagga gatgcgcagc catgagaccc ggacacccgt tattctgatg
     8521 acggcctatg ctgaagtgga aaccgccgtc gaagcactac gctgcggagc cttcgactat
     8581 gttatcaaac cgtttgatct cgatgagttg aatttaatcg ttcagcgcgc tttacaactc
     8641 cagtcaatga aaaaagagat ccgtcatctg caccaggcac tgagcaccag ctggcaatgg
     8701 gggcacattc tcaccaacag cccggcgatg atggacatct gcaaagacac cgccaaaatt
     8761 gccctttctc aggccagcgt cttgattagc ggtgaaagcg gcaccgggaa agagttgatt
     8821 gccagagcga ttcactacaa ttcgcggcgg gcaaaggggc cgttcattaa agtcaactgc
     8881 gcggcgctgc cggaatcgtt gctcgaaagt gaactgtttg gtcatgaaaa aggtgcattt
     8941 actggtgcac aaaccttgcg tcagggatta tttgaacgtg ccaacgaagg tactctgctc
     9001 ctcgacgaaa ttggcgaaat gccgctggta ctgcaagcca aattactacg cattctacag
     9061 gaacgggaat ttgaacggat tggcggccat cagaccataa aagttgatat ccgcatcatt
     9121 gctgccacca accgcgactt gcaggcaatg gtgaaagaag gcaccttccg tgaagatctc
     9181 ttttatcgcc ttaacgttat tcatttaata ctgccgcctc tgcgcgatcg ccgggaagat
     9241 atttccctgt tagctaatca ctttttgcaa aaattcagta gtgagaatca gcgcgatatt
     9301 atcgacatcg atccgatggc aatgtcactg cttaccgcct ggtcatggcc gggaaatatt
     9361 cgagagcttt ccaacgttat tgaacgcgct gtcgtgatga attcaggccc gatcattttt
     9421 tctgaggatc ttccgccgca gattcgtcag ccagtctgta atgctggtga agcaaaaaca
     9481 acccctgtcg gtgagcgtaa tttaaaagag gaaattaaac gcgtcgaaaa acgcatcatt
     9541 atggaagtgc tggaacaaca agaaggaaac cgaacccgca ctgctttaat gctgggcatc
     9601 agtcgccgtg cattgatgta taaactccag gaatacggta tcgatccggc ggatgtataa
     9661 cgccaaaact tgctatgcag aaatttgcac agtgcgcaat tttctgcata gccgctcatt
     9721 ctccttatga atccccatct aatttatcct tttctattca attagttaaa taacaacatt
     9781 caataatctc attctggcac tccccttgct attccctgac tgtacccaca acggtgtatg
     9841 caagagggat aaaaaatgaa aacaaaattg atgacattac aagacgccac cggcttcttt
     9901 cgtgacggca tgaccatcat ggtgggcgga tttatgggga ttggcactcc atcccgcctg
     9961 gttgaagcat tactggaatc tggtgttcgc gacctgacat tgatagccaa tgataccgcg
    10021 tttgttgata caggcatcgg tccgctcatc gtcaatggtc gagtccgcaa agtgattgct
    10081 tcacatatcg gcaccaaccc ggaaacaggt cggcgcatga tatctggtga gatggacgtc
    10141 gttctggtgc cgcaaggtac gctaatcgag caaattcgct gtggcggagc tggacttggt
    10201 ggttttctca ccccaactgg tgtcggcacc gtcgtagagg aaggcaaaca gacactgaca
    10261 ctcgacggta aaacctggct gctcgaacgc ccgctgcgcg ccgacctggc gctaattcgc
    10321 gctcatcgtt gcgacacact tggcaacctg acctatcacc ttagcgcccg caactttaac
    10381 cccctgatag cccttgcggc tgatatcacg ctggtagagc cggatgaact ggtcgaaacc
    10441 ggcgagctgc aacctgacca tattgtcacc cctggtgccg ttatcgacca catcatcgtt
    10501 tcacaggaga gcaaataatg gatgcgaaac aacgtattgc gcgccgtgtg gcgcaagagc
    10561 ttcgtgatgg tgacatcgtt aacttaggga tcggtttacc cacaatggtc gccaattatt
    10621 taccggaggg tattcatatc actctgcaat cagaaaacgg cttcctcggt ttaggcccgg
    10681 tcacaacagc gcatccagat ctggtgaacg ctggcggaca accgtgcggc attttacccg
    10741 gcgcagccat gtttgatagc gccatgtcat ttgcgctaat ccgtggcggt catattgatg
    10801 cctgcgtgct cggcggtttg caagtagacg aagaagcaaa cctcgcgaac tgggtagtgc
    10861 ctgggaaaat ggtgcccggt atgggtggcg cgatggatct ggtgaccggg tcgcgcaaag
    10921 tgatcatcgc catggaacat tgcgccaaag atggttcagc aaaaattttg cgccgctgca
    10981 ccatgccact cactgcgcaa catgcggtgc atatgctggt tactgaactg gctgtctttc
    11041 gttttattga cggcaaaatg tggctcaccg aaattgccga cgggtgtgat ttagccaccg
    11101 tgcgtgccaa aacagaagct cggtttgaag tcgctgccga tctgaatacg caacggggtg
    11161 atttatgatt ggtcgcatat cgcgttttat gacgcgtttt gtcagccgat ggcttcccga
    11221 tccactgatc tttgccatgt tgctgacatt gctaacattc gtgatcgcgc tttggttaac
    11281 accacaaacg ccgatcagca tggtgaaaat gtggggtgac ggtttctgga acttgctggc
    11341 gtttggtatg cagatggcgc ttatcatcgt taccggtcat gcccttgcca gctccgcgcc
    11401 ggtgaaaagt ttgctgcgta ctgccgcctc cgccgcaaag acgcccgtac agggcgtcat
    11461 gctggttact ttcttcggtt cagttgcttg tgtcatcaac tggggatttg gtttggttgt
    11521 cggtgcaatg ttcgcccgtg aagtcgcacg gcgagtaccc ggttctgatt atccgttgct
    11581 cattgcctgc gcctacattg gttttctcac ctggggtggc ggtttctctg gctcaatgcc
    11641 tctgttggct gcaacaccgg gcaacccggt tgagcatatc gccgggctga tcccggtggg
    11701 cgatactctg ttcagtggtt ttaatatttt catcactgtg gcgttgattg tggtgatgcc
    11761 atttatcacc cgcatgatga tgccaaaacc gtctgacgtg gtgagtatcg atccgaaact
    11821 actcatggaa gaggctgatt ttcaaaagca actaccgaaa gatgctccac cgtccgagcg
    11881 actggaagaa agccgtattc tgacgttgat catcggcgca ctcggtatcg cttacctggc
    11941 gatgtacttc agcgaacatg gcttcaacat caccatcaat accgtcaacc tgatgtttat
    12001 gattgcgggt ctgctgctac ataaaacgcc aatggcttat atgcgtgcta tcagcgcggc
    12061 agcacgcagt accgccggta ttctggtgca attccccttc tacgcgggga tccaactgat
    12121 gatggagcac tccggtctgg gcggactcat taccgaattc ttcatcaatg ttgcgaacaa
    12181 agacaccttc ccggtcatga ccttttttag ttctgcactg attaacttcg ccgttccgtc
    12241 tggcggcggg cactgggtta ttcaggggcc gttcgtgatg cccgcagccc aggcgctggg
    12301 cgctgatctc ggtaaatcgg taatggcgat cgcctacggc gagcaatgga tgaacatggc
    12361 acaaccgttc tgggcgctgc cagcactggc aatcgccgga ctcggtgtcc gcgacatcat
    12421 gggctattgc atcactgccc tgctcttctc cggcgtcatt ttcgtcattg gtttaacgct
    12481 gttctgacgg caaccctaca gacagaagga atataaaatg aaaaattgtg tcatcgtcag
    12541 tgcggtacgt actgctatcg gtagttttaa cggttcactc gcttccacca gcgccatcga
    12601 cctgggggcg acagtaatta aagccgccat tgaacgtgca aaaatcgatt cactacacgt
    12661 tgatgaagtg attatgggta acgtgttgca agccggactg gggcaaaatc cggcacgtca
    12721 ggcgctgcta aaaagcggac tggctgaaac ggtgtgcgga ttcacggtca ataaagtgtg
    12781 tggttcgggt cttaaaagtg tggcgcttgc cgcccaggca attcaggcag gtcaggcgca
    12841 gagcattgtg gcggggggta tggaaaatat gagtttagcg ccctacttac tcgatgcaaa
    12901 agcacgctct ggttatcgtc ttggagacgg acaggtttat gacgtaatcc tgcgcgatgg
    12961 tctgatgtgc gccacccatg gttatcatat ggggattacc gccgaaaacg tggctaaaga
    13021 gtacggaatt acccgtgaaa tgcaggatga actggcgcta cattcacagc gtaaagcggc
    13081 agccgcaatt gagtccggtg cttttacagc cgaaatcgtc ccggtaaatg ttgtcactcg
    13141 gaagaaaacc ttcgtcttca gtcaagacga attcccgaaa gcggattcta cggctgaagc
    13201 gttaggcgca ttgcgcccgg ccttcgataa agcaggaaca gtcaccgccg ggaacgcgtc
    13261 tggcattaac gacggtgctg ccgctctggt gattatggaa gaatcttcgg cgctggcagc
    13321 aggcctgaat cccctggctc gcattaaaag ttatgccagc ggtggcgtgc cccccgcatt
    13381 gatgggtatg gggccagtac ctgccacgca aaaagcgtta caactggcgg ggctgcaact
    13441 ggcggatatt gatctcattg aggctaatga agcatttgct gcacagttcc ttgccgttgg
    13501 gaaaaccctg ggctttgagc ctgagaaagt gaatgtcaac ggcggggcca tcgcgctggg
    13561 gcatcctatc ggtgccagtg gtgctcgtat tctggtcaca ctattacatg caatgcaggc
    13621 acgcgataaa acgctggggc tggcaacact gtgcattggt ggcggccagg gaattgcgat
    13681 ggtgattgaa cgattgaatt aacatgtaaa aacgcccgat agcgaaagtc atcgggcgtt
    13741 ttcttgaaca ttgacagtaa agataacctc attaatcacc agtcaaaact tttcaccagc
    13801 gtcagttcgc cagcattacg catcggtaca ataaaagttt cctgtttctc attgaccgac
    13861 ccttcatcgg tgatcagcgt cagttgggcc gtagttaatt ccgtttcgct gcgtccgcca
    13921 tagtagttga tatacacctg ataacggccg tgaactggcg ctggcatggc gaaaatctcg
    13981 gggccgtacc ctgtcgtgac atccatatcc agtgcaccac tgtttttcag cacggtgtta
    14041 ccgtaccagg catgttcacc gtcaggcgta acgacatgaa gatcgaggtc ggtattgtcc
    14101 gtatcccacg agagaaccag ccgtaaacgt gcgcgaatcg ccccagcacc cggcgttgag
    14161 taaaactgca ttttttgtcg gctttgccca tctgggctga tgacctgcac gctgttgctg
    14221 ccttcagtga aaatataagg gcgtgcaaaa gaaccatctg attcaattct ctgcggcata
    14281 ctggcaccgt tgaccaccag ccggccctgc tgaactttac ccgccgcagc ataattcttt
    14341 attttgccgc ggatctgcgc tgagatattt tgatcatctg ccatgttgac ggacgacgcc
    14401 gggtaattga tcgtttgtga aaaactggca tcttcaccct ctgccggatg ccagccagag
    14461 agcggtgcat caatctcgac ttcctgcaca ccttcactgt gagcgacagg cgaaagcgcc
    14521 accagtaaca acggaagaaa aatctttcgc atagcattag tccattaata attgtcgggt
    14581 cagcgtttcg atatagttct catcaagccc ggtgggataa ccatcaaagg ccaggtgcag
    14641 atactcatgg gttaaatcca gccgatcctg aagcgtaaag aagttacgaa taaacagccg
    14701 tttctgctgc cgatcggtat aggggaaacc tgagactaaa cgacagaccg caaacacgtc
    14761 tggttcgttg tatcccgtct caccttgtaa tatacgccgc cactgcggca tttttttcgc
    14821 cagccaggct ttcgctttgg gtaataactg gcaggttgag cgtggcgctc cccagcggct
    14881 aaggttgttg tcaggataag caaacgcaag tatctgatcg taacgctctc cctgccccgc
    14941 ctgggcagta gcgtgccgcc aggaaagcgt cccttcggta acccggctgc catgataatg
    15001 aaccggatcg cccgcataga tgagatcctg cgtccaggcg gtcatcgtcc gcgcgcctac
    15061 agtggctggc gaagctgaaa cgcgctgggt agcgctactg tccgggatgc tcaggcaatc
    15121 accatcgcga tcagcgtttt gctgtaaata cgtgcgaatc gccacggtca tggctttcgc
    15181 tgcctcagga ggcgtacttt tcgcctcgcg atccagaact ctggcaacgt actcttcacg
    15241 atcgagatgc gactgtaatt tcagtttgcc ctttaccgtc agtaacgttg tctcaccgtg
    15301 gctgacaaat gtcatgtggt taccattggc aaacgtcaca cgatagcgcc cattgagcac
    15361 gcctggtttg actgccgtcg tactcttttc ctcagtaact ttcttcagcg gataacgggc
    15421 aaacagctcc acctctacac attgcccact ggcaacttgc gtcggcactg gcaatacccg
    15481 attaagcacc gtcgcataac gggtcagtac cgttttgctg gtgccactgc cagtaaccca
    15541 cagcggtgta ccatcggtta accagccggc aaaaccaccc tgacggattt gctgtttatc
    15601 atcggcaaac cagctccagg ttttcacccg tacccgacta ccgagcatgg atgcgacacc
    15661 gattttcgcc tcgtccagca ccacatccag taagacttcc tgagctttgt tttgcgcggg
    15721 taacgtcgct aatgaatcaa gcagcgattt aaccgttacc gaggtttctg gtttcagcgt
    15781 cggaagagac gctaaccacg caggtgcctg acgctgttgc cagtattgcc cccacatatc
    15841 tgcaccaata tgcaaccgtt gcggcgcaaa atagagtcca caggagcgta ccagcgcggt
    15901 atcgcgcgtt atgctctccc cagcctgaca acaataaacc tcttccgggg agtttccgcg
    15961 acactggtag ggctgctccg gttgatgggt atcctccagc caggcgtaaa catacagttt
    16021 ccataaactc cctaacggcg tggtgagcgt gtcaggtaat gctgagactt tggtcacgcc
    16081 tgatgacgac atctgataca gttgatcgtg ctgcgcaccg cgcagcacca gttgtaacgg
    16141 cgtctcctcc gcaagcgttg gcagagtaac caacgccaat agccagacga ttctgcgcca
    16201 gttcacttat ttcacctgca tcccggtcca ttcactccct gccgcaacac tttgctgtgc
    16261 aggtgcatag gaacgcacat aacgagcagg aggcagaacg aattgccctt tttgcgagaa
    16321 gcgcagcaaa tggcggaaag tgaccgttcc cgtcagttct ttcaccggca ccatataggc
    16381 cagttcgccc atttcattac gcgctttttc cagcaattgc ccctgctgtt ttccagcgtt
    16441 aggtttattg accgaaatgc cccatgttgt gcgctcaacg tcggctcccg gcgggagcgg
    16501 tacttccacc tgaccatagc gcagaactgc atcctgctcg ctggtaagcg taatttcatc
    16561 gagatacagc gcatcgctgt caatctcatt gctggtcacc ggctgcagag taaagctcat
    16621 ctcttcttca ccggggataa gccgatacaa ctggcgttca acggtcaccg gaatgttact
    16681 ttgttgagcc gtttttgccg gttcacgcca gcggacctgc acattttgcg gcgataattc
    16741 gtcaccaaaa gagagaatgt ccggcacacc ctgaccaacc caacgccagt cttcgccccc
    16801 tccagttaac ttatgtttgg cccatgcgcc cgcaggcgca ggcaacacaa ctggaggcat
    16861 cgtcgccata tatttcgcca gccagttcat ggccagcgcg cgctcaatag tggattgctc
    16921 agcggtcaaa ccacttaaaa tagcggccgc ctgggtagcg tcgccaccgc tattaagcag
    16981 cacaaccgtg tgcgccagcg gctggttact gtttgccgct ttatcccacg catattgctg
    17041 aacctgtggc atcacagtgg acttcaactg cgcttttttc agcaacgtca tggttaagac
    17101 gtttgccacc gcatctgcca gtggagattc aggtgtatcg aggatcatgc tgtcattgat
    17161 atcgctggtg tctacctcag agaaatcttt agttttagtt ccgcgccggg cgatagcttc
    17221 atccaaccct ttcaacaacg ttttgcacgg cagattcatc tcctgcgccc acgccagcac
    17281 cagcgcccga tgcaataacg gcatgttatc cgcctgctcc gcgtagctgt cgagcatatg
    17341 ctgccagtat tccggttgct gcgttacgcc gagcgcctgg ctggcctgcc agtcggcgta
    17401 ccatgcccat gccgtgagga aggcgtcgcc attgccatct tcaccccacc aggtaaagcg
    17461 cgctccgggc cccgccagtt gcatcagccg cagacggtta tcctgaatca tctgacgaat
    17521 gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc caggcgagac tgagcgggat
    17581 cagacggcta ccggtgttga tcacgccacc ccacggttca tcgactaatg catcaagatt
    17641 gttacggaaa atctcctgcg gcgtttcgct actttgtagc cggatattgc tcgcctgctc
    17701 gggcaacgtc agcgcattat cgccaccgcc gagcatgacg tttttctgtt gttcaacggg
    17761 ccagctgtta tccacaaaag acagttttgt gctaatgctg tcctgtactt gcccattttg
    17821 ttgcagttct gcacttaaca agccagattg ctgaatattc tgcgtcagcg aaatataatt
    17881 cgcccctttg tgcagcgtca gcgtctggcg catctcagca cctgcaaatt tagtcaccag
    17941 cgccaccggt tcgttatcct gctgactgaa gataaacagt cctgccgccg gtttgtcgcc
    18001 catgcgatac accgttggca tactccattt catgtagaga cttttttccg aacgcagata
    18061 agcacgcccc tgcccgacca gcccgtcgcc gttcatccca cgcgcggtga tgcgccagcg
    18121 ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg ccttgtttat cggttgtgag
    18181 tgacggcatc caggccgcgg tatccacctc ttcacgccgt ggacgttcaa gcatttttac
    18241 tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga gcgcctgatc
    18301 gtagctgata aatgacaggc tggagctggt acgcacattg ttacgcccca gcggatagaa
    18361 aaatttgccg atattcggcg cgatttccgg ttgcagcgcg tagatcattt catcgaccac
    18421 gcctaccgtt aactgcgcag aaacaggttt acctttcagc gacgaggtta attcgacatt
    18481 gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat ccagctgggg
    18541 aacggcaact ttaatcccgg cgttctgaaa actgtactga ccgttacggg tatacagcac
    18601 cgaaaaagtg atgttaggcg caaaggaatt gctcaccggt acgcgggctt catactgggt
    18661 atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gatagcagcg acttctgttc
    18721 cacgcgatcg cgttccagcg tcaataatgc ttcatcaatt ggctccggga aggtaatcag
    18781 catcttcgcg gtttcgccag gctggtacag cgttttatct gccacgatat ctaccgtacc
    18841 agtatgcgac gtgctgccct taccgctgac tgcatggctt aacccggcga gaattaagcc
    18901 gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga cggtaaagga
    18961 tttgccgcct gacggtagct ctccgctgtg gctcgtgcgg tcttcgagac gcaaccattc
    19021 atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca caaccagctc
    19081 gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc cacgttcaat
    19141 gaggatctct ttggtggtgg taactcgata cgccgcgccg tcactggcgg agacggttaa
    19201 caaataacgg ctcggtttat cggcggcggg gagattcaac gccacatgac cattatcgtc
    19261 ggaaaccgtt tcactgcctt ccagcgacac cgggaaacgt cctgcataac gcaaatcgtt
    19321 accaaccatt gataattgct gagcgcgcaa acttaactgc acgcgagcat tttttaccgg
    19381 ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag ttttgaactc
    19441 ttttttatcg agagctaaac caatctcaaa atggggtttg atgtagtttg ccacgcgaaa
    19501 actgctgcta tagacctgtt tgcggtaagc aagacgtaac tcataacctc cggcgacggc
    19561 attttctggc aggcggaaac tcccctgccc gccattacgc gcatccagcg tgacattgac
    19621 ggtttgcaac agactgccgt tggcgtccag caccgaaagc ttcgccggtg cgctgacgat
    19681 gggggatgaa tgcaacggat cgtggaactc gcggcccatc actttaacat cgacacgatc
    19741 gcctgcgcga tatagcgggc gatcggtaaa gatatacaag cgggtgttgt agatttcgct
    19801 ttcgtagaaa aagttctcgg agacaaaaac gccgccttca gcatccttac ccagaatata
    19861 tgaacgttct ggcgatatat gttgtaactg caaggtaccg ctgccatcgg tcacaccgcg
    19921 ggtcatcacg ccaagaccgt cagtccacaa gatctctgag ccgggcttcg cttcaccctg
    19981 ttttttcccc gcggtccaca ccagaagctc gttacctgac actttgctaa gcgccacggt
    20041 atcggaaaca aacaccaccg tcgtcgcccg atacccaccc accatcgcct cgacgaggta
    20101 cagtcccggc tcttgtttgc cgagaggaat atagatgttg cccggctgcg gcgaaatgaa
    20161 attgctggat gcgccttcca gttttacccc ttgttgcggc tcgaccggtt tagcctgcca
    20221 tagtgggtaa cggaactgtt ccactagtgg atattttttc agcggtgaaa actggttgtt
    20281 ctgtacataa cgggaaggtt taataatggc attaccgagc tgtaattcgg gtaacgcctg
    20341 agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc gagatttgcc
    20401 gtaccagtta tcccacagcc aggtcagcgt attgttcagt ccgtcgccca gatattgcgg
    20461 ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg ggtcaggaat
    20521 acgatacagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc gataatcacg
    20581 ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc tgtcagcgag
    20641 caaaaagaat gttcccccgg cgggcggcac atagttactg gaaggaagcg aatcatcagc
    20701 attagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg cgccagaaaa
    20761 tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca tcgggtgtcc
    20821 ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt tttcgtggcg
    20881 cttccggtgt ggtagatgat gtaacgcccc atccagacca ttaagtgctg ggcatcgccc
    20941 tgatcgaaaa aaatcatatc gccaggcagt gcctggttta tgtcctggcc aataaactgg
    21001 ctgttgtact gaatcaaatt aatcgcggtc acataggggc cggttttccc attcccctga
    21061 ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg cggcaaatac
    21121 tggttagata aaccgttact ttttagccat ttactgtcgt gaactttcag cgcctcgttc
    21181 gccgcaaatc gcaccaggcc cgcgcaatcc tgttgatacc agcgtgggct tggcccctgg
    21241 cggagctgtt cctgtgcaat gcgcacaaac cacgcgcgaa acagcccgga ttgttcgaca
    21301 tttagcattt cactatgggc aacaacacaa tacagccagc aaatcagcac cagcagcccg
    21361 tgtctcatag cggttgccag gttatgggta gccactgcca ggcggcaccg ggttccatct
    21421 gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc ggcattaata
    21481 aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg gtcagcgttt
    21541 cgttacgcag cagtttcgcc attccttgtg gattgatata gagcggaacg atgccatcag
    21601 tgggtattac atccaccatt gccgggcggg ttttattcag tgcttgaagg gcgttattaa
    21661 ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc gacacgcgga
    21721 aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga tactgaccgt
    21781 atcgggaact cacttcgcga cgccagattt gcgcttcgcc ctgctgagtc tggcttaccg
    21841 gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta aacagtttcc
    21901 ctggcaattg cgcctgttcg gcagtgccat caaactgacc aacaaacagt ggggtttgca
    21961 attttgagtc ttcataccag cacagccccg cgccaccgtc taacgcccca ttcagcttgt
    22021 cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa tacggcaccg
    22081 ccacacagaa gctggctccg gcaggcatac tgttccatac ctgcgtaaaa tcgaaactgg
    22141 catctacgct ggcggattca tcgtttaacg ccagaaaact gtgccagccg tcgttaccca
    22201 tttcgaagcg cacgccagca aaagaaggca ttaatcgctg gtagccaaac cccagcaacc
    22261 tggcgctgac tacgatgcgc tggcgtactg gcgttttttc ggcagtacgc gcttccaggc
    22321 caaagcgggt ttcccagcgt tttttaccac tcaacaaatc acttgcgatc gccgtagctt
    22381 cggtatcctg ttggtcatct ttaaacagca tatccgaact ggaaaacgcc aatattttgt
    22441 cctgataagt cgcaaacatc aggacgttat tgccgttata gcgcaactga taaacaggaa
    22501 tagtttcact atttatcttg atgctactga tttccgtttt acttaactgg ctgtcgctgg
    22561 tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg ataagcacca
    22621 tataatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgca ggctgatcta
    22681 agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca atgcggcgaa
    22741 tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg aaatcttcgc
    22801 taagaacatc gtggagaaac ggaatggtaa ggagatcttt gggaagctgg ctcagagagt
    22861 cgctgtcgag aaagaggtcc ggctcattga gatcgatttg cagattgttg tgcaccacca
    22921 gtggcgacaa cgttttttct ggcccactgc cagagtattg taatgcccat acgccagcgg
    22981 atagcagcgc tattgcccca aaaccaacaa ggccataaaa ccgccagcct ttcgcctttt
    23041 tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg ggcacagcca
    23101 aactttaccg cgccctaata cgacaaaagc ccagacttta cagcctggac ttttcaattc
    23161 aaaaaaggga gatggctccc ttttggcatg aagcgatgaa attattcttc ttctggctcg
    23221 tcgtcaacgt ccacttccgg agcgatttca tcgtcccctt ccgcggcact gccgtcgatg
    23281 gtatccagat cttcctcgtc aaccggttca gcaacacgtt gcagacccac cacgttttca
    23341 tcttccgaag tacggataag gattacgccc tgggtgttac ggcctacgat gctgatttcc
    23401 gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg gtcgcagtca
    23461 tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat ggagataacc
    23521 cctttcgtcg cacgcgactt ggttgggtat tccgccactg cggtacgttt accataaccg
    23581 ttctgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga gacgacttta
    23641 tcgccttcgc ctaaacgaat accacgaaca ccggtggtgt tgcagcccat cgcacggaca
    23701 gaagactctt taaagcgcac cactttacct tcagcggaga acagcattac ttcgtcttcg
    23761 ccgctggtca ggtcaacgcc gatcagctca tcgccttcaa ccagtttgat cgccacttta
    23821 ccggcggtac gcagacggtt gaactcggtg aggacggttt tcttcaccgt accgttagcg
    23881 gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat cgcggtgata
    23941 cgttcgtcct gttccagcgg cagcaggttg acgatcggac gaccacgcgc gccacgagta
    24001 gcttctggca actggtagac cttcatcgaa tagacgcggc cacggctgga gaagcacaga
    24061 atatggtcgt gagtgttcgc caccagcagt cggtcgataa agtcttcttc tttaatacgt
    24121 gcggcagatt tacctttccc gccacgacgc tgcgcttcat attcagaaag cggctgatac
    24181 ttcacgtaac cctggtgaga aagcgtgacc accacatctt cctgggtgat cagatcttcc
    24241 aggttgatgt cggcgctgtt agcggtgatt tcggtacgac gtttgtcacc gaactgttca
    24301 cgaaccagct ccagctcttc acggatcact tccatcagac gatcggcgct accaagaata
    24361 cgcaacagtt ccgcaatctg atccagcagc tctttgtatt cgtcgagcag tttttcgtgc
    24421 tcaaggccgg tcagtttctg cagacgcaga tccagaatcg cctgagcttg ctgttcggtc
    24481 aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg acgcgcagca
    24541 tcgtcgcccg cacgttccag catcgcagca acgttgccca gctgccacgg atttgcaacc
    24601 agcgcggttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat gatcgggtcg
    24661 atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg agctttacgc
    24721 agttcgaaaa tagtacgacg ggtcactact tcacggcggt gacgaacaaa cgccgcgatg
    24781 atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat gttgataccg
    24841 aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc accgaccgca
    24901 tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc acgcagcgcg
    24961 ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat caggcgcgct
    25021 ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt tttggcgtca
    25081 acttctactt cggcacgggc gcggatatat accttgccgc gaccggtacg gtaagcttct
    25141 tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc cgggatgtgt
    25201 tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca accgttgatg
    25261 acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat accggaagaa
    25321 ccgttcacca gcaggttagg aattttggtt ggcatgacgt ccggaatttt ttccgtaccg
    25381 tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag ttcatgggca
    25441 attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc gtcgatggaa
    25501 ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg cgccatacgg
    25561 acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat tacgtcacca
    25621 acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac gttcatggcg
    25681 taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag cgcacggcca
    25741 acaatgaccg acatcgcata atccagatag gagctcttca gctcttcctc aatgttgacc
    25801 ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg tatcccggat
    25861 tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata cgctttattc
    25921 acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag tgccaatgaa
    25981 tgccgaaaaa tcgccggtaa accacaacgt agaccacgaa gagatcgcta aatttgaagc
    26041 cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgctgcacc gcattaaccc
    26101 gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa aggtgctcga
    26161 tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgggagggcg cgacggtgac
    26221 cggtctggat atgggctttg agccattgca ggtggcaaaa ctgcacgcac tggaaagcgg
    26281 cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac atgccggaca
    26341 gtatgacgtg gtgacctgta tggagatgct ggagcacgtc cccgatcctc agtcagtggt
    26401 cagagcctgt gcgcaactgg tgaaaccggg cggcgatgtc tttttctcga cgcttaaccg
    26461 caacggcaag tcatggctga tggcggtggt tggtgcggaa tatattttgc gcatggtgcc
    26521 taaaggtaca catgatgtga agaagtttat taaaccggca gaattgctgg gctgggtgga
    26581 tcagaccagt cttaaagaac ggcatatgac cgggctgcat tacaacccta tcactaacac
    26641 ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga ataagtgagg
    26701 ttgttgtttg gattcatccg gcaaccgcgc caagcctgat gcgacgcttg ccgcgtctta
    26761 tcaggcctac aaatgctccc cgtaggcctg ataaggcgtt tacgccgcat ccggcaacag
    26821 tgccggtcag acagcgatta ccatttcact gtcatcgaca aaaaccctgc cgtctgggca
    26881 aaatcatcac tccctttctg ccacgccacg ctgccgcgca gcgaaactcg ctgactgata
    26941 ttgcccgtta ttcccacttt tatttcaccc cgttgcttca ccgcatcgtc actgatagtg
    27001 ctgccatctt cttcaatttc cgtcgcatgg ggatcgtgat aataattcag atctaacgtt
    27061 ggcgtgacac caacagcagt acgccattcg ctgtgtaaac ccagccgcgt ctgaatatca
    27121 tcaccctgcg attgtgacac gcgcgcatgg ttagcggcgg taaaatcatc ctgatgcacg
    27181 ccctgataaa tcacctgcgt ctgcggttca atcaccacac catgccccgg taaccactga
    27241 taaccggctt ccagcgaggc gataatcccc gacgagtggt aatgatccac gccatcgtct
    27301 tgttcggaaa catcattgtt aaaccacaca tattgcagcc agctatccaa ccaggccccc
    27361 tgcttctgat taccgtgctg ataccagctt gaggtcagcc caacggcata accgtggttc
    27421 tggttatcgg cgcgagttcc ggtcatattc gagcggctgt cgccctggtt atcgctgtag
    27481 ccaccaacaa tcccaagcat ccactcgcca tcatcacccc agtgcccgct aaacaggttg
    27541 ccgctaagct gcaccgtaga agtgtcttca tgctgagcca actgccctac cgccgtgtaa
    27601 tgataacgcc cgccgataac gcgtaaattc agcgtctgac catcgccacc agcgtgatcg
    27661 cgtcgctcca tcacaaatgc ctgatttgcc gcccgcaggt tattaaaata tccaccaact
    27721 ttggcattca acaccggctg gtaggcaggc gtaggttccg ggtcgggggt tgggtcggga
    27781 tccggcgtgg gatcaggatc tggagtcggg tctggatcag gcggcggcgt cggattgact
    27841 tcttgtgatc gcagatacca gtcgttgtta tcttccacca gcgtgtagtc atacgccccc
    27901 atattgacgt agccgcttcc tgccagactg aactgcgcat tgttttgaaa ctgcgcggga
    27961 tcagctgcga aatcaactac tttaatacct gtagatgtcg gctcaccaat acccgtaatg
    28021 gggttaatta ccacggccgt gttgccagca gtattaccgt tcaataccag ttggtcactt
    28081 gctgagtcat cgccgtttaa ttcgctatcg agcagtagcg taccgccgcc ggtataatca
    28141 ccatttaccg tcagcgtgtt gccagcgacg ccattttgca gactgacagc accggacgta
    28201 ttggtcagat tgccattaac cgtcacgtcg gaaagataag tcaggctgtc agtttcaagg
    28261 ttggtattaa gctcacctgc cagcgtcacg ccatcggtgg cattcaccag cgtaccgtcg
    28321 ccgctaaaca gcgaggcatc cagcgcctgg ttcgtatcct gctgcgtcaa tcgcaacaca
    28381 gtaccatcgg ttatgtcgat atcgccgcca gaggtggcat cgattgaacg aatatcctga
    28441 ttttgcccgg tttcaaaaac accgtcttcc cccacccaca gtgaagaaac ataaggaata
    28501 atattctccg cttcaccttt tagcgttccg tcctccacac gtaccgcaga cgtcgcagtg
    28561 ccgctggcgg tcaactccag cgtcccgccg ccacttttag tcaacgtgct tacgctatcc
    28621 agttgttgcc cggtactatc cgccatcaac ccgccccatt gcgtatctac gccatcctca
    28681 acgtgtattt cgccatcagc gcgcatttca atattcctgc cctgacctgt tgtagccgag
    28741 cgagtgtcaa catcagtcgt cacatccatt tcactgtgtt gttggggatc agtgaagatc
    28801 actgaacggt tgtaacttgt gtcccccaat tgcgaattat cgctgacttc aagcgtaccg
    28861 gaggcgattt gcgtattacc gagataaccg ttgctattcg ccagcgttac gcgctgatcc
    28921 cccgcattaa tgacgacata aacgccattc tggctggcat cactgccgcc aatatgtaaa
    28981 tcatcgccac tgctgatatc aacgacatca ttactgccaa tcactgtccc cccgctgccg
    29041 ctgatttgca gaccgtcatt gtatgagcta tcgccatccc aggtggtgaa atcagagaga
    29101 tccaatactc cgccatttag cacgatcgat tgcggatcgc tctgcattgc ccgtaaatcg
    29161 gcctgattcc ccgctagcga caatacggcg ttatcttcca ccacaatatc gccggttaat
    29221 gccatcgact gcgcccctgt caggagatag ctgccatctt gcgctaccgt caactgtcct
    29281 tcaccctgaa tcgagccgga aaaaccgccc tggtttactg taacattgcc gcctgcgtcg
    29341 atattcaaaa tgccattagc aaaaccggtt aatgagtgca caaacgtttg ttgtgtattg
    29401 ccaacattca gttctgcctg atactcagaa tgaacagtgc tgccgaccat cagaccaaag
    29461 caatcctgcg gatcgctctg acagtgcgtg tcgccgacat tcattaactc atcgctgcgc
    29521 cccaacgtca cctcgccatt ctggatactc atctcgccgg taaacgcatt attgtcggca
    29581 ttgaggacta actctcccgc tccctcttta acgatgactc ccgttccggc aagagaatca
    29641 atcgcgccat cgttgctggt attgcctatt accagcgttt tatcggcggc aatgttaaat
    29701 tcagccacac tatgaccgat atacataaag ccccccgccg ctgccgtcgc cgaatgatca
    29761 tatcccgagg cgctgttatt ttcatcaatc atcacaccat tgttttggtc ataattatca
    29821 tcaacagaaa tatcaataag atacggggat gtggtgctgc tggtataaat tgctccgcca
    29881 taaccttctg catagttatt ggtaaaggag gtattattaa ttatcgtttt tcctgaaaga
    29941 tgtgtgttat ccgtactatt atccgtaaca tcaatggcgc cgccatcccc atcactataa
    30001 cttgttgatg tatatgcttg gttattatta aaagcatcat cacttaaata gacgtcatta
    30061 ttgatggtat aaattgcacc gcctttgccg tcattagcga tgttattgtg aaatacggcg
    30121 ttagtgacac gtaaatcggc ggcaccggta tcattggtac cggcagaata gattgctcct
    30181 ccatagcctc ctgcaacatt actgtcgaaa ataacattcg caagatttat cgttgagttt
    30241 tctttagcaa agatagcccc accattatta tattcgccac taaccgagtt atcagcaaac
    30301 agggtcatcc ctgtttcatt ttgtggtgat atcgtaaatt cagcactttg ctgcaaaaat
    30361 actgcgccac cgctggcatt attggccata cccgaaaaaa tcaaccattg cccatccgcg
    30421 atgctccagt cctgcatatt gcctgaaagg ctttgcctgc tggcctgaca gctcgctttg
    30481 atatcatatc cctggcatga atcaatgacc gctgcggcac cgttaccaga gaaaagagat
    30541 gcaatcattg acgggagtaa agataaatac tccttgcgta gaaagataat ccgcatattc
    30601 atctaaccat cattttctat aagatggcgt atttaatcgc attatgcgat atagggtatt
    30661 tttcttttgt aaattctgcc cctgatgtaa taactatttt gttattatct gataattaat
    30721 ttagatgcat cacaattcaa attattaact ttaagg
//
LOCUS       DAZFRE010000059        30229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFRE010000059 DAZFRE010000000
VERSION     DAZFRE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.059"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHKUI_002984"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHKUI_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6599145.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHKUI_002985"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHKUI_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6599146.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            832..1992
                     /gene="pheA"
                     /locus_tag="ACHKUI_002986"
     CDS             832..1992
                     /gene="pheA"
                     /locus_tag="ACHKUI_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6599147.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2035..3156)
                     /gene="tyrA"
                     /locus_tag="ACHKUI_002987"
     CDS             complement(2035..3156)
                     /gene="tyrA"
                     /locus_tag="ACHKUI_002987"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6599148.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVSDAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQSFQSESRVLLRQANDNRQ"
     gene            complement(3167..4237)
                     /gene="aroF"
                     /locus_tag="ACHKUI_002988"
     CDS             complement(3167..4237)
                     /gene="aroF"
                     /locus_tag="ACHKUI_002988"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6599149.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /gene="yfiL"
                     /locus_tag="ACHKUI_002989"
     CDS             4447..4812
                     /gene="yfiL"
                     /locus_tag="ACHKUI_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6599150.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENTSQ
                     LHEVWRKGADENASTIRLN"
     gene            4961..5479
                     /gene="yfiR"
                     /locus_tag="ACHKUI_002990"
     CDS             4961..5479
                     /gene="yfiR"
                     /locus_tag="ACHKUI_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6599151.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5469..6695
                     /gene="dgcN"
                     /locus_tag="ACHKUI_002991"
     CDS             5469..6695
                     /gene="dgcN"
                     /locus_tag="ACHKUI_002991"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6599152.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            6711..7193
                     /gene="yfiB"
                     /locus_tag="ACHKUI_002992"
     CDS             6711..7193
                     /gene="yfiB"
                     /locus_tag="ACHKUI_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6599153.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7270..7617)
                     /gene="rplS"
                     /locus_tag="ACHKUI_002993"
     CDS             complement(7270..7617)
                     /gene="rplS"
                     /locus_tag="ACHKUI_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6599154.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7659..8426)
                     /gene="trmD"
                     /locus_tag="ACHKUI_002994"
     CDS             complement(7659..8426)
                     /gene="trmD"
                     /locus_tag="ACHKUI_002994"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6599155.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8457..9005)
                     /gene="rimM"
                     /locus_tag="ACHKUI_002995"
     CDS             complement(8457..9005)
                     /gene="rimM"
                     /locus_tag="ACHKUI_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6599156.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9024..9272)
                     /gene="rpsP"
                     /locus_tag="ACHKUI_002996"
     CDS             complement(9024..9272)
                     /gene="rpsP"
                     /locus_tag="ACHKUI_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6599157.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9409..10770)
                     /gene="ffh"
                     /locus_tag="ACHKUI_002997"
     CDS             complement(9409..10770)
                     /gene="ffh"
                     /locus_tag="ACHKUI_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6599158.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10937..11728
                     /gene="ypjD"
                     /locus_tag="ACHKUI_002998"
     CDS             10937..11728
                     /gene="ypjD"
                     /locus_tag="ACHKUI_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6599159.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            11749..13035
                     /gene="yfjD"
                     /locus_tag="ACHKUI_002999"
     CDS             11749..13035
                     /gene="yfjD"
                     /locus_tag="ACHKUI_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6599160.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQVLMMPLVWLLNAITRILMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13090..13683)
                     /gene="grpE"
                     /locus_tag="ACHKUI_003000"
     CDS             complement(13090..13683)
                     /gene="grpE"
                     /locus_tag="ACHKUI_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6599161.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKD"
     gene            13806..14684
                     /gene="nadK"
                     /locus_tag="ACHKUI_003001"
     CDS             13806..14684
                     /gene="nadK"
                     /locus_tag="ACHKUI_003001"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6599162.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQTRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14770..16431
                     /gene="recN"
                     /locus_tag="ACHKUI_003002"
     CDS             14770..16431
                     /gene="recN"
                     /locus_tag="ACHKUI_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6599163.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16580..16921
                     /gene="bamE"
                     /locus_tag="ACHKUI_003003"
     CDS             16580..16921
                     /gene="bamE"
                     /locus_tag="ACHKUI_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6599164.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16983..17273)
                     /gene="yfjF"
                     /locus_tag="ACHKUI_003004"
     CDS             complement(16983..17273)
                     /gene="yfjF"
                     /locus_tag="ACHKUI_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6599165.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17263..17700)
                     /gene="ratA"
                     /locus_tag="ACHKUI_003005"
     CDS             complement(17263..17700)
                     /gene="ratA"
                     /locus_tag="ACHKUI_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6599166.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17871..18353
                     /gene="smpB"
                     /locus_tag="ACHKUI_003006"
     CDS             17871..18353
                     /gene="smpB"
                     /locus_tag="ACHKUI_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6599167.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18567..18929
                     /gene="ssrA"
                     /locus_tag="ACHKUI_003007"
     tmRNA           18567..18929
                     /gene="ssrA"
                     /locus_tag="ACHKUI_003007"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19612..20589
                     /gene="glaH"
                     /locus_tag="ACHKUI_003008"
     CDS             19612..20589
                     /gene="glaH"
                     /locus_tag="ACHKUI_003008"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6599168.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLPELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSTGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLCRELMRQRGYFAYATHHYQTHQ"
     gene            20609..21877
                     /gene="lhgO"
                     /locus_tag="ACHKUI_003009"
     CDS             20609..21877
                     /gene="lhgO"
                     /locus_tag="ACHKUI_003009"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6599169.1"
                     /translation="MYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            21900..23348
                     /gene="gabD"
                     /locus_tag="ACHKUI_003010"
     CDS             21900..23348
                     /gene="gabD"
                     /locus_tag="ACHKUI_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6599170.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPTGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            23362..24642
                     /gene="gabT"
                     /locus_tag="ACHKUI_003011"
     CDS             23362..24642
                     /gene="gabT"
                     /locus_tag="ACHKUI_003011"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6599171.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMLRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            24879..26279
                     /gene="gabP"
                     /locus_tag="ACHKUI_003012"
     CDS             24879..26279
                     /gene="gabP"
                     /locus_tag="ACHKUI_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6599172.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            26300..26962
                     /gene="csiR"
                     /locus_tag="ACHKUI_003013"
     CDS             26300..26962
                     /gene="csiR"
                     /locus_tag="ACHKUI_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6599173.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVNLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTATVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(26963..27412)
                     /gene="kbp"
                     /locus_tag="ACHKUI_003014"
     CDS             complement(26963..27412)
                     /gene="kbp"
                     /locus_tag="ACHKUI_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6599174.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILIAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(27496..27654)
                     /gene="yqaE"
                     /locus_tag="ACHKUI_003015"
     CDS             complement(27496..27654)
                     /gene="yqaE"
                     /locus_tag="ACHKUI_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6599175.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            27837..28136
                     /gene="ygaV"
                     /locus_tag="ACHKUI_003016"
     CDS             27837..28136
                     /gene="ygaV"
                     /locus_tag="ACHKUI_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6599176.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            28146..28670
                     /gene="ygaP"
                     /locus_tag="ACHKUI_003017"
     CDS             28146..28670
                     /gene="ygaP"
                     /locus_tag="ACHKUI_003017"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6599177.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQSGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQSLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(28717..29121)
                     /gene="stpA"
                     /locus_tag="ACHKUI_003018"
     CDS             complement(28717..29121)
                     /gene="stpA"
                     /locus_tag="ACHKUI_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6599178.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            29789..>30229
                     /gene="alaE"
                     /locus_tag="ACHKUI_003019"
     CDS             29789..>30229
                     /gene="alaE"
                     /locus_tag="ACHKUI_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6599179.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
BASE COUNT         7574 a         7795 c         7383 g         7477 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattt ccatcacaca
      181 ttttgacatc aggaacgtta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacaccgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgta
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aatcgggggg ccttttttat tgataacaaa aaggcaacac tatgacatcg
      841 gaaaacccgt tactggcgct gcgagagaaa atcagcgcgc tggatgaaaa attattagca
      901 ttactggcag agcgacgcga actggccgtc gaggtgggaa aagccaaact gctctcgcat
      961 cgcccggtac gtgatattga tcgtgaacgc gatttgctgg aaagattaat tacgctcggt
     1021 aaagcgcacc atctggacgc ccattacatt actcgcctgt tccagctcat cattgaagat
     1081 tccgtattaa ctcagcaggc tttgctccag caacatctca ataaaattaa tccgcactca
     1141 gcacgcatcg cttttctcgg ccccaaaggt tcttattccc atcttgcggc gcgccagtat
     1201 gctgctcgtc actttgagca attcattgaa agtggctgcg ccaaatttgc cgatattttt
     1261 aatcaggtgg aaaccggcca ggccgactat gccgtcgtac cgattgaaaa taccagctcc
     1321 ggtgccataa acgacgttta cgatctgctg caacatacca gtttgtcgat tgttggcgag
     1381 atgacgttaa ctatcgatca ttgtttgttg gtctccggca ctactgattt atccgccatc
     1441 aatacggtct acagccatcc gcagccattc cagcaatgca gcaaattcct taatcgttat
     1501 ccgcactgga agattgaata taccgaaagt acgtctgcgg caatggaaaa ggttgcacag
     1561 gcaaaatcac cgcatgttgc tgcgttggga agcgaagctg gcggcacttt gtacggtttg
     1621 caggtactgg agcgtattga agcgaatcag cgacaaaact tcacccgatt tgtggtgttg
     1681 gcacgtaaag ccattaacgt ttctgaccag gttccggcga aaacgacgtt gttaatggcg
     1741 accggacaac aagctggtgc actggttgaa gcgttgctgg tactgcgcaa tcacagtctg
     1801 attatgaccc gtctggaatc acgtccgatt cacggtaatc cgtgggaaga gatgttttat
     1861 ctggatattc aggccaatct tgaatcagcg gaaatgcaaa aagcattgaa agagttaggg
     1921 gaaatcaccc gttcgatgaa ggtattgggc tgttacccaa gtgagaacgt agtgcctgtt
     1981 gatccaacct gatgaaaaag tgccggatga tgtgaatcat ccggcactgg attattactg
     2041 gcgattgtca ttcgcctgac gcaataacac gcggctttca ctctgaaaac tctgtgcgta
     2101 atcgccgaac cagtgctcca ccttgcggaa actgtcaata aacgcctgct tatcgccctg
     2161 ctccagcagc tcaatcgcct cgccgaaacg cttatagtaa cgtttgatta acgccagatt
     2221 acgctctgac gacataataa tgtcggcata aagctgcgga tcctgagcaa acagtcgccc
     2281 gaccatcgcc agctcaaggc ggtaaatcgg cgaagagagc gccagtagtt gctcaagctg
     2341 aacattttct tctgccaggt gcagcccata agcaaaagta gcaaagtggc gcagagcctg
     2401 aataaacgcc atattctgat cgtgctcgac agcgctaata cgatgcaacc gagcgcccca
     2461 gacctgaatt tgctccagaa accattggta tgcttccggc ttacgtccat cacaccagac
     2521 cacaacttgc tttgccaggc taccgctgtc cgggccgaac atcgggtgta accccagcac
     2581 cgggccatcg tgcgccgcca gcatggcctg taatggtcca tttttcaccg atgccagatc
     2641 aaccagaata caatctttcg gtaaaggcgg taatttgccg ataacttgct cagtaacgtg
     2701 gatgggcaca ctaacaatca ccattccggc atcggaaaca atatcagccg ctcgatccca
     2761 gtcatgttgc tccagaatcc gcacctgata acccgagagt gtcagcatct tctcgaacag
     2821 acgtcccatc tgaccgccac caccgacgat aaccaccggg cgcagtgacg gacaaagcgt
     2881 tttaaatcct ttgtcgtttt cactggagta agattcacgc atcacccgac gcaaaacatc
     2941 ctcaatcaga tctggcggta cacccagagc ttccgcctct gcacgacgcg aggccaacat
     3001 agatgcctcg cgctccggaa cataaatagg tagtccaaag cggcttttca cctcgcccac
     3061 ttcagcaacc agttccagac gcttcgctaa taaattcagc agcgctttat cgacttcatc
     3121 aatttgatcg cgtaatgcgg tcaattcagc aaccataata aacctcttaa gccacgcgag
     3181 ccgtcagctg cccgttcaga tcctgatgaa tttcacgcag caaggcatcg gtcatttccc
     3241 agctaatgca ggcatcggtt acggatacac cgtatttcat ttcactgcgc ggttgctcgg
     3301 aagactgatt gccctcgtgg atattacttt cgatcatcag accaataatt gagcgattgc
     3361 catctttgat ttgagcaacc acggattctg ccaccgcagg ctgacggcga taatctttat
     3421 tggaattacc gtggctgcaa tctaccatca gagacgggcg cagtcccgcc tgttccatct
     3481 ctttttcaca ttgcgcaaca tccgcagggc tatagtttgg cgctttacca ccgcgcagga
     3541 tcacatgccc gtccggattc ccctgagttt gtagcaacgc aacctgccct gcctggttaa
     3601 tgccaacaaa acggtgcggc tgggcggcgg cacgcatagc gttaattgcc gttgccagac
     3661 tgccatcggt gccgttttta aaaccaaccg gcatggaaag cccggaggcc atttcacggt
     3721 gagtttgcga ttccgttgta cgagcaccaa ttgctgacca gctaaacaga tcgcccaggt
     3781 attgcgggct attcggatct aacgcttccg tcgccagtgg cagtcccata ttcaccagct
     3841 caagcagcaa tttacgcgcg atctgcagcc cggcttctac atcaaaagag ccatccatat
     3901 ggggatcgtt aattaaccct ttccagccga cagtggtacg gggtttttca aaatagacgc
     3961 gcattaccag atagaggcta tcgctgacct ctgcggcaag ggctttaaat cgacgagcat
     4021 attccagagc agtttccgga tcatgaatgg aacaaggacc acatactacc agcagacgag
     4081 gatcgcgccc ggcgataata tctgaaatgg ttttacgcga gtcagcaatc tgggcttctt
     4141 gttgcaagct taatggaaaa gcggccttca gttgttccgg agtcattaaa acctgttcgt
     4201 cggtaatatg tacgttattc agcgcgtctt tctgcatgat ggcgatcctg tttatgctcg
     4261 tttgcgatag ttgatcctca gcgaggatga cgtaacgata acacataaag taaagttttc
     4321 aatccatatt tagtacatat ttctttacat aagcaattta ttcgcgcttt ttccccttat
     4381 ttctgtataa ataaatttac accagcttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaaatt tatcgccccc ttgttggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgttggtatg
     4561 gaagatgcga tatcgggcag cgccattaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaagggcaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttctggtaaa agctttcctg ccagctgcaa taatgttgaa
     4741 aatacaagcc aactgcatga agtatggcga aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaccccc ccaaaatcaa ttaagcatga gattttactc attatgtttt
     4861 atttctcaca ttgatgacga ccgcatgaaa ggatgatatt ttaatgagca ttctgaaaaa
     4921 atatcgatgc atttcgagcg aagatggtga agatccctga atgcgttttt ctcaccgact
     4981 gttcttactg ctgattctcc tgctgacggg agcaccaata ctcgctcagg aaccttctga
     5041 cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgg
     5101 gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccttaca
     5161 agaaaatgct gctacatctt taccttatct gcctgtcatc attcatactc aacaagaggc
     5221 gataatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga
     5281 attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg
     5341 cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa
     5401 cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg
     5461 gaagaaaaat gatggataac gataattctc ttcataagcg ccccacgttt aaaagagcat
     5521 tacgcaacat cagcatgacc agcatattta tcactatgac gctgatctgg ctgctgcttt
     5581 ccgtgacgtc agtgctaacc ctgaaacaat acgcgcaaaa aaacctggca ctgacagcag
     5641 caacaatgac ttacagtctg gaagcagctg tcgtttttgc cgatggccct gcagcaactg
     5701 aaacactggc agcgctgggc cagcaaggcc aattttcaac tgcagaagtg cgtgataagc
     5761 agcaaaatat tctggcatcc tggcattaca cccataagga accaggcgat actttcagca
     5821 atttcataag ccactggctc ttccctgccc ccatcattca gccgattcgc cacaatggtg
     5881 aaaccattgg cgaagtacgc ttaactgctc gcgacagttc aatcagccat tttatctggt
     5941 tttcgctcgc tgtcctgacc ggttgtattc tgctggcatc aggcatcgca attaccctca
     6001 cccgccattt gcacaatggc ctggtggaag cgctgaaaaa tatcaccgat gtcgtacatg
     6061 atgtgcgttc taaccgcaat ttttcccgac gagtttcgga agaacgtatc gctgagtttc
     6121 accgcttcgc tctcgacttc aacagtctgc tggatgaaat ggaagagtgg cagcttcgtt
     6181 tacaggccaa aaatgcgcag cttctacgta ccgcgctaca tgacccatta accgggctgg
     6241 ctaaccgcgc agcgtttcgt agcggcatca acacgttgat gaacaattcc gatgcccgaa
     6301 aaacgtcggc gttactattt cttgatggcg ataatttcaa atatatcaat gatacctggg
     6361 gtcatgcgac gggcgataga gtcttgattg aaatagcaaa acggttagct gaatttggcg
     6421 ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt cgctatggtg ctctatgatg
     6481 tacagtcgga atctgaagtg cagcagatat gctcagcact gacacaaatt tttaatctcc
     6541 cgtttgatct tcataatggc catcagacta ccatgacatt aagcattggt tacgcgatga
     6601 ctattgagca cgcctctgcg gaaaatttac aagagcttgc cgatcacaat atgtatcagg
     6661 ccaaacacca gcgtgccgaa aagctggtga gataacaagg atatatcgat atgataaagc
     6721 acctgctagc acccctgatt ttcacctcgc taatacttac tggctgccag tcccctcagg
     6781 gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta tggatttact gaatccgccg
     6841 gcgactggtc gctgggctta tcagatgcca ttctgtttgc aaaaaatgac tacaaattgc
     6901 tcccggaaag ccagcaacag atccaaacca tggcagccaa attggcctcg acagggctaa
     6961 cacatgcccg tatggatgga cacaccgaca actatggtga agacagttac aacgaagtct
     7021 tatcattgaa acgggcgaat gtcgtggccg atgcatgggc tatgggtgga caaattccgc
     7081 gcagcaatct caccactcag ggtttaggaa aaaaatatcc catagccagt aacaagaccg
     7141 cccagggccg cgccgagaac cgccgcgtcg cagtggtgat tacaacccct taaaacacga
     7201 gcaaacagac aaaaaaaggg ccagccaatt ggccagccct tcttaacagg atgtcgctta
     7261 agcgaaatct tagttaagac gctctttgat acgagcagcc ttaccagtac gctcacgcag
     7321 gtagtacagt ttagctttac gaacagcacc acgacgtttg acagaaatgc tgtcaactac
     7381 cggagagtga gtctggaaga cacgctcaac gccttcgccg ttggaaattt tacgaacagt
     7441 gaatgcagag tgcagaccgc ggttacgaat agcgataacc acgccctcga atgcctgcag
     7501 acgttttttg gaaccttcaa caacccatac tttcacttcc acggtatcac ccggacggaa
     7561 ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc ttaataatgt tgctcataat
     7621 ttaatctctt atcctgggta aactgatatc tcgggggctt acgccatccc atcatgttta
     7681 tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca accttgcttg ctcttcagtc
     7741 agagccaggt tttccagaag ttcaggtctt ctaagccagg tacggcccag cgactgtttc
     7801 aaacgccagc gacgtatctc ggcatggttg cccgacagta acactggcgg aacttccatc
     7861 ccttctaaca cctcaggccg cgtatagtgc gggcaatcca gcaatccttc agcaaaggaa
     7921 tcttccgttg ccgaggcttc atgtcccagt actcccggaa taaaccggga aacggagtca
     7981 atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt aatcgccgat tgaccattct
     8041 tcgtcaattt cggtttggat cacgcgctca tctatacctt cgtagcgacc acacaccaga
     8101 atcaattttt gattcgttgc cagttcgctg acgcccgctt gatcaagctt gcgtccctgt
     8161 ggtgacagat aaatcacctt tgcgccttca cccgccgcgg cttttgctgc atgaatggcg
     8221 tcccgcaagg gttgcaccat cattaacatc cccggtccgc cgccgtaagg acgatcgtcc
     8281 acggtacggt gccggtcatg cgtgaagtcg cgaggactcc agctctggat gctcagcagg
     8341 ccatttttaa ctgcccggcc agttaccccg taatcggtaa ttgcgcggaa catttcagga
     8401 aacaggctaa ttatgccaat ccacatagcg ccgtctttta ccgtttatcc ggtggtttaa
     8461 aaaccaggat cccaatctac ttcgattgaa cgtgtagtga gatcgacttt cttgataacc
     8521 tgcccatcga ggaacggtac gagacgttcc ttgataccaa acgcatcttt caggtttgcc
     8581 ttaatgacga gaacgtcatt agatccggtt tccatcatat ctacgacttt accgagatcg
     8641 tagccttcag tggttactac ctggcagccc atcaggtctt tccagtagta gtccccctct
     8701 tcaagctgag gcaactgcga tgaatccacg acaatttcac aattcgtcag caggttcgcc
     8761 gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt cctgattgtg gtgcttccag
     8821 ctttccagct ggacttgctg ccactgaccc gccttctgga taaaccaggg ctgatagtca
     8881 aaaatgcttt cggcgtcttc ggtggaagaa aacactctga gccacccacg aataccgtaa
     8941 gacgacccca tttttcccaa aacgatggga tcaacaggtg cttgcgcggt gagttgtttg
     9001 ctcatcatga ccaccgtgac agattaagct gctttgttta cttctttgat cagcgcagca
     9061 acgcgatcag aaatagttgc gccctggcca acccagtgag cgatgcgatc cagatccagg
     9121 cgagtgcctt cttctttttc gctagcgatt gggttgaaga aaccaacgcg ctcgatgaag
     9181 cgaccgttgc gtgcattacg gctgtcagcg acaacaacct ggtagaacgg acgcttttta
     9241 gcgccgtgac gtgctaaacg aatagttacc ataacatcct cttgtgtgaa taaaacaacc
     9301 ggaccccatc gaggaacgga gtccggtgtc atattaaaag cccgaaaatt ttactgattt
     9361 ctggagaaaa tgcaatcagc agttaataac tctatcgcat aaggtcattt agcgaccagg
     9421 aaatcccggc ggcatcatcc ctttcatgct tctcatcatc ttcgccatcc cgcccttttt
     9481 cattttcttc atcatgcgct gcatgtcgtc gaactgtttc agaagacggt taacgtcctg
     9541 cacctgcata ccgcaaccgg cagcgatacg acgcttacgc gaacctttga tgatttctgg
     9601 cttagcgcgc tctttcatcg tcatcgagtt gatgatggct tccatacgca ccagcacttt
     9661 atcgtccatc tgtgacttga cgttatccgg gatctgcccc atgcccggca gcttgcccat
     9721 caggctagcc atgccgccca tatttttcat ctggcgcagc tgctcaagaa agtcgttgag
     9781 atcgaagccg tcgccttttt tcagcttgct ggctaatttc tctgcctgcg cgcggtcaac
     9841 cttgctttcg atatcttcga tcagtgacag tacgtcgccc atgccgagaa tacgggaggc
     9901 aatacggtcc ggatggaatg gctccagcgc ctcggttttc tcgccaacac cgaggaactt
     9961 gatcggtttg ccagtgatgt gacgaataga gagcgccgca ccgccgcggg catcgccgtc
    10021 cactttggtc aacactacac cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt
    10081 ggccgcatcc tgaccggtca tggcatcgac cacaaacagg gtttcaaccg gattaatcga
    10141 cgcatggact tgtttgatct cgtccatcat cgcttcgtca acgtgcagac gaccggcagt
    10201 atccaccagc agcacgtcgt agaatttcag tttggcttct ttcagcgccg cgttaacgat
    10261 atctaccggc ttctgaccaa catcagaagg gaagaaatca acgcccacct gctctgccag
    10321 cgtctcaagc tgtttgattg ccgccgggcg atagacgtcg gcagaaacca ccagcacttt
    10381 cttcttgtgc ttctcgcgca ggaacttacc gagtttacca acgctggttg ttttaccggc
    10441 accttgcagg cccgccatca gcacgaccgc aggcggttgc gcagccaggt tcagggtctg
    10501 gttctcttcg cccatcgccg caaccagttc gttacggaca attttgacga actcctgccc
    10561 cggcgtcagg ctcttattaa cttcatgacc aaccgctttc tcttttacgc gattgataaa
    10621 ctcacgcact accggcagag cgacgtccgc ctccagcagc gccatgcgca cttcgcgcag
    10681 cgtatctttt acgttgtctt cagtgaggcg tccacggcca ctgatattgc gcagcgtgcg
    10741 cgacaaacga tcggttaaat tatcaaacat tgtctctcgc ctggggtgga aacggttggc
    10801 cgcaatcgcg acacatcatc agtattttgc cgcagtataa catgaaggcg tctttgttgt
    10861 tatgcaacgg ttggagcagc gttcacctga cgctatactg cttctctttc ttattgctca
    10921 aactgtcgac attactatgc ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag
    10981 tcttgcgctg attgttcccg gtctgctgca aaaaaacggc ggctggcggc gcatggctat
    11041 tatttctgcg gtcatcgcgc tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc
    11101 cgacggtgat agcggacaaa acctcagcct gctgaacgtt ggttcattgg tcagtttgat
    11161 gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt ggctggttgt tgctacccat
    11221 tgtctatgcc tttgcgctta tcaacctggc gctggcgacc ttcatgccca atgaatacat
    11281 cacccatctg gaagcaacgc cggggatgct ggtgcacatt ggcttatcgc ttttttccta
    11341 tgccacgcta attatcgctg ccctgtacgc gctgcaactg gcgtggattg attaccaact
    11401 gaagaacaag aagctggcgt ttaaccagga aatgcctcca ttgatgagta tcgagcgtaa
    11461 aatgttccat atcacgcaga ttggcgtggt gctgctaacg ctcactcttt gcactggcct
    11521 gttctacatg cacaacttat ttagcatgga aaatatcgac aaggccgtgc tctctatcgt
    11581 ggcgtggttt gtctatattg tgctgctgtg gggacattat catgaaggat ggcgtggacg
    11641 ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg acactggcct acttcggcag
    11701 ccgaattttt cagcagttaa tcagctaaac ccagaaaagg agtttcccct ggaacacatt
    11761 tctactacta cgttgatcat tattctgatc atcatggtgg tcatttcagc ctatttttcc
    11821 ggttcagaaa ctggaatgat gacactcaac cgctatcgcc tgagacacat ggcgaagcaa
    11881 ggtaatcgct cggcaaaacg cgtcgaaaaa ttactgcgca agccagaccg cctgataagc
    11941 ctggtgttaa tcggcaataa tctggtcaat atccttgctt ccgcactcgg cactattgtc
    12001 gggatgcgct tgtatggcga tgctggcgtg gcaattgcga ctggcgtgct gaccttcgta
    12061 gtgctggttt ttgctgaagt attgccgaaa accattgccg cactgtaccc ggaaaaagtc
    12121 gcctatccga gtagttttct actggcgccg ctacaagtcc tgatgatgcc gctagtctgg
    12181 ttactaaatg ccatcacccg aattttgatg cgcatgatgg gcatcaaaac cgatatcgtg
    12241 gttagcggct ctttgagcaa agaagagttg cgtaccatcg tgcacgaatc acgctcacaa
    12301 atttcccgtc gcaatcagga tatgctgctg tcggtgctcg atctggaaaa gatgaccgtc
    12361 gatgacatca tggtgccgcg cagtgaaatt atcggtattg atatcaacga tgactggaaa
    12421 tcgattctgc gccaactctc ccactcacct cacgggcgca tcgtgctcta ccgtgattcg
    12481 ctggacgacg ccatcagtat gctgcgtgtg cgtgaagcct ggcgactcat gtcggagaaa
    12541 aaagagttca ccaaagaaac catgctacgc gccgcggacg agatctattt tgtcccggag
    12601 ggtacgccgc tcagcacaca gttggtgaag tttcagcgca acaaaaagaa agtcggcctg
    12661 gtcgtcaacg agtatggaga cattcaggga ctggtgacgg tcgaagatat tctcgaagag
    12721 attgtcggcg atttcaccac gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa
    12781 aacgacgggt cggtgattat cgatggcacc gccaacgtgc gagaaattaa caaagccttt
    12841 aactggcatc taccggaaga tgatgctcgt actgttaacg gcgtcattct ggaagcgctg
    12901 gaggagatcc cggtagcagg cacccgcgtg cgtataggcg agtacgatat cgatattctc
    12961 gacgtacagg acaatatgat taagcaggta aaagtttttc ctgtaaaacc gctgcgcgag
    13021 agtgtggcgg agtaacgaaa acggcccggc attcgcatgc tgggccgtga tttattacga
    13081 aagcagaaat taatcttttg ctttcgctac agtaaccatc gccgcacgaa tcgtacgacc
    13141 gttcagcgta taacctttct gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga
    13201 ttccaccatt gcgatggcct gatgcacatt tgggtccagt gggacgttgg tttcggcgat
    13261 cacttcaacg ccaaacttac gcacaacatc cagcatcgac ttcagcgtca gctcaatacc
    13321 ttcaaccatc gcggacatat ccgggttagc tttatcagcc acttccagcg cccgatccag
    13381 gctatcaatc accggcagca attcgttgat gaatttctcc agcgcgaatt tgtgagcttt
    13441 ttcaatatcc agttcagtac gacgacgcag gttttccatt tcggctttta cgcgcaaaat
    13501 gccgtcacgt tcacgggtct gggcttcagc cagctgagct tcgagattcg caattttttc
    13561 atcacgcgga tccacctgct cagcagaagc ttctggctca actgcctcaa tctcttcgtg
    13621 ctgatccatg ataatttctt ccggggcttg cccctcaggc gttttctgtt ctttactact
    13681 catgaatttc tccgcgtttt tttcgcattc atctcgctaa cttcgcttat tatggggatc
    13741 agtttcaggg tttcaaggga agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg
    13801 gaaaaatgaa taatcatttc aagtgtattg gcattgtggg acacccacgg caccccactg
    13861 cactgacaac acatgaaatg ctctaccgct ggctgtgcac aaaaggttac gaggtcatcg
    13921 ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg
    13981 agattgggca acaggctgat ctcgcggttg tcgttggcgg cgacggtaat atgctgggcg
    14041 cggcgcgcac gctcgcgcgc tatgatatta aagttattgg aatcaaccgt ggcaacctgg
    14101 gtttcctgac tgaccttgat cccgataacg cccagcaaca attagccgat gtgctggaag
    14161 gccactacat cagcgagaaa cgttttttgc tggaagcgca agtctgtcag caagattgcc
    14221 agacacgcat cagcacggcg attaacgaag tggtacttca ccctggcaaa gtggcgcata
    14281 tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt ttcgcagcgt tccgatggac
    14341 tgattatttc gacgccaaca ggctccaccg catattccct ctctgcgggc ggtcctattc
    14401 tgacaccctc tctggatgcg attaccctgg tgcccatgtt cccgcatacg ttgtcagcac
    14461 gaccactggt cataaacagc agcagcacga tccgtctacg tttttcgcat cgccgtaacg
    14521 acctggaaat cagttgcgac agccagatag cactgccgat tcaggaaggt gaagatgtcc
    14581 tgattcgtcg ctgtgattac catctgaatc tgattcatcc gaaagattac agttatttca
    14641 atacattaag caccaagctc ggctggtcaa aaaaattatt ctaattttac gccagcctct
    14701 ttactgtata taaaaccagt ttatactgta cacaataaca gtaatggttt ttcatacagg
    14761 aaaacaacta tgttggcaca actgaccatc agcaactttg ctatcgttcg tgagcttgag
    14821 attgattttc atagcggcat gaccgtaata actggcgaga ccggcgcggg taaatctatt
    14881 gcaatagatg ccctcggcct ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc
    14941 ggcgctgctc gtgccgacct gtgcgcccgt ttttctctga aagatacgcc agcggccctg
    15001 cgttggctgg aagaaaacca gcttgaagac gggcatgaat gtttgcttcg tcgcgtaatc
    15061 agcagcgatg gtcgctcccg tggtttcatc aacggtacag ctgttcctct gtcacaactg
    15121 cgcgaactgg gtcagttgct gattcagatc catggtcagc acgctcatca gttactcacc
    15181 aaacccgagc accaaaaatt cctgcttgat ggctatgcca atgaaacctc tcagctccag
    15241 gaaatgaccg cacgttatca tttgtggcat caaagctgcc gtgacctcgc gcatcatcaa
    15301 cagttaagtc aggaacgcgc cgctcgtgcg gagctgctgc aataccaatt aaaagaactt
    15361 aacgaattta atccgcagcc tggagagttt gagcaaatcg acgaagagta caaacgtctg
    15421 gcgaacagcg gtcaattgct gaccaccagc cagaatgcat tggcattaat ggctgacggt
    15481 gaagacgcaa acctgcaaag tcaactttac acggctaaac aactggtgag cgaattgatt
    15541 ggcatggaca gcaaactgtc cggcgtactt gatatgctgg aagaggctac catccagatt
    15601 gctgaagcca gtgatgaact gcgccactac tgcgatcgtc tggatctcga tcccaaccga
    15661 ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc tggcacgtaa acatcacgtc
    15721 agccctgagg cattgccaca gtattaccag tcgctactgg aagaacagca gcaactggac
    15781 gatcaggccg actcacaaga aacgctcgcg ctggcggtaa cgaaacatca tcagcaggca
    15841 ctggaaacgg cgcgcgcgtt acaccagcaa cgccagcatt atgcaaatga gttggcacag
    15901 ctgatcaccg atagtatgca tgcactctct atgccacatg ggcagtttac gatcgatgtt
    15961 aaatttgacg agcatcacct gggcgctgac ggcgctgatc gtattgagtt tcgggtaacc
    16021 accaacccag gtcagccaat gcagcctatt gccaaagtcg catccggtgg tgagttgtcc
    16081 cgcatcgcat tggcaatcca ggtcattacc gcgcgtaaaa tggaaacccc ggcactgatt
    16141 tttgatgaag tggatgtagg gattagcggc ccaacagcgg cagttgtcgg caaactgcta
    16201 cgtcaacttg gcgaatcaac tcaggtgatg tgtgttaccc acctgccaca agtcgcggga
    16261 tgtggtcatc aacactattt tgtcagcaaa gaaactgatg gtgcgatgac agaaacgcat
    16321 atgcaatccc tggataaaaa agcgcggtta caagagttgg cgcgcctgct tggtggcagt
    16381 gaagtcacac gtaacacact ggcgaatgcg aaagaactgc ttgcggcgta aacttttttc
    16441 ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac cggaaagcaa aaggttttaa
    16501 agtgatgaaa ggtctattat catcggcata ttacagatga gccacgtact gctcgggccc
    16561 gaaaaggaat caaatcacta tgcgctgtaa aacgctgact gctgcagcag cagtactatt
    16621 gatgttgacc gcaggctgtt ccactctgga gcgagtggtt taccgtcctg acatcaacca
    16681 ggggaactat ctgaccgcta acgacgtatc caaaatacgt gttggcatga cgcaacaaca
    16741 agttgcgtac gcattgggta caccgctgat gtccgatcca tttggtacaa atacctggtt
    16801 ctatgtcttc cgccagcaac ctggtcatga aggtgtaacg cagcaaacgc tgacgctgac
    16861 ctttaacagt agcggtgtgt tgaccaacat tgataacaaa cctgcgctga gtggtaacta
    16921 ataataaagt tgctctcaaa gacgttaaaa aaggtgctca atgagcacct tttttctgtc
    16981 tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg
    17041 agaggacgat aaatctccac ccgatcgcca tcatgcacaa tatcgctgag ttttgccgga
    17101 cggctgtaaa tgccgacttt gtttttagtt aaatcgatat cggtacgcaa ttccagcaaa
    17161 ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct gcaacgtcac gcgctgcagg
    17221 tactgcttct caggtagcgc ataagccacc tcaacggcaa ttttacctgg cactgtagac
    17281 ctcttttgca cgaacagtaa aagcctgcac catattagct gccagctctt taaacacacg
    17341 accaaaggcg agttcaatca acttattggt aaactcaaag tcgagatgaa actcaatacg
    17401 gcacgcgtcc tggctcaacg gcgtaaactt ccaaccgccg atcaatttct taaacggtcc
    17461 atcaaccaga ctcatgagaa tactttgatt actggtcaac tggttgcggg ttgtaaacgt
    17521 tttgctgatc ccagccttag acacatctac cgcagcagtc atctgcccag gagtggactc
    17581 cagaatccga cttccggtac aacccggcaa aaactgagga taagactgaa cgtcattcac
    17641 taactgatac atttgctccg cgctgtaggg caccagtgcg gtccggctaa tctgaggcat
    17701 aacaatttcc atcaacaaaa atccaacaaa taatatcatt tatcctgctg taaaaaaaac
    17761 gctatcccgg cgctgggtaa catcgggttc atgctaagat agagccttgt cccccgcagg
    17821 attgatatgg ggtgttttcg atttcagatt accgatgatt cacgacgctt atgacgaaga
    17881 aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat
    17941 actttatcga agaagagttc gaagcgggac ttgccctgca aggctgggag gttaaatccc
    18001 tgcgcgcagg aaaagccaat atcagcgaca gctatgtcct tctgcgcgac ggagaggcat
    18061 ttctgtttgg cgctaacatc acgccaatgg ctgtggcctc cacgcatgtg gtgtgcgatc
    18121 ctacccgtac ccgcaagtta cttctcaacc agcgcgaact ggactcattg tacggtcgcg
    18181 tcaatcgaga aggctatacc gtagtggcgc tctccctgta ctggaaaaat gcctggtgca
    18241 aagtgaaaat cggcgtcgcc aaaggtaaga aacagcacga taaacgttca gatatcaaag
    18301 aacgcgaatg gcaggtggat aaagcgcgta tcatgaaaaa cgcccaccgt taaacctata
    18361 taagcgatac taatcaaatc ctcaccgcgc ctccctatcc ggtggcgcga atgaacatct
    18421 tatcgactgt caaatctgac acagatgtag ccatcccatt gcttaatcga aaaaaatcat
    18481 actatgcagg cgctaattcc aggatgataa cgccttttgg gctagtcatg ctgctcataa
    18541 atctggtata cttaccgtta cacattgggg ctgattctgg attcgacggg atttgcgaaa
    18601 cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag ccgcaaaaaa tagtcgcaaa
    18661 cgacgaaaac tacgctttag cagcttaata acctgcttag agccctctct ccctagcctc
    18721 cgctcttagg acggggatca agagaggtca aacccaaaag agatcgcgtg gaagccctgc
    18781 ctggggttga agcgttaaaa cttaatcagg ctagtttgtt agtggcgtgt ccgtccgcag
    18841 ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc gaggatgtag gaatttcgga
    18901 cgcgggttca actcccgcca gctccaccaa tcatgattgg acggtgtaag gactacacca
    18961 acaaaaacag gaagttataa gtctcagcag gacaccgacc agacggtgag gagacaaaaa
    19021 aggatacgca acggagtcgc gactccctat gacattaaag cccgctgatg cgggcttttt
    19081 tattggtcct gacccgccct gaatagaatt catccaatta cagtccagac atatacagta
    19141 tccctcagct cagtatcagc ttctcgacaa atggtcactg ttatttaccc gttgccagaa
    19201 agagaataat ttgctcccat agccagcctt cccggaatct gtatattcat taatattgtt
    19261 atcgattcca tcaagtaaca taggttggat gcgattgatc ttactcacat tagtcgacac
    19321 ggaactcagt aagttgaact ctggaaaagc agtttaaata gcatttaaac aaatgccacc
    19381 agttcgaaaa agagtataag cacacttaat atattgtttt aattattcat ttcctttcaa
    19441 cccaattctt atccctcaca ttatttgtta ttaatttgtt atttttgatc acaataagaa
    19501 aacaatatgt aacttttatg cgcatttttc agaaatgtag atatttttag attatggcta
    19561 cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca
    19621 ctgaccgccg tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc
    19681 atcccgtcgg cgcaatcccc gcgtctgccg gaactcacct tcaccgaaca gacgaccaat
    19741 cggttcctcg aacaggttgc cgagtggccc gtgcaggcgc tggagtacaa atcgtttctg
    19801 cgttttcggg taggcaaaat tctcgacgat ctgtgtgcga atcagctgca accgctgctg
    19861 ttgaagaccc tgttaaaccg cgctgaaggt gcgctgttga tcaatgcggt gggtatcgat
    19921 gatgtcgcgc aggcggatga gatggtgaag ctggcgacgg cggtggcgca tctgattggt
    19981 cgctccaatt ttgacgccat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtt
    20041 gataactcag acagctatct tcgtcagccg caccgcgtaa tggagctgca caacgacggc
    20101 acttacgttg aagagatcac tgattacgtg ctgatgatga aaatcgacga gcaaaacatg
    20161 cagggcggta actcgctgct gctgcatctc gatgactggg aacatctgga ccactttttc
    20221 cgccatccgc tggcgcgccg tccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc
    20281 aaagatgtct tccatccggt gttcgatgtc gatcagcagg gccgcccggt gatgcgctat
    20341 atcgaccagt ttgtccagcc gaaagacttc gaagaaggcg tgtggttgag cgagctttcg
    20401 gacgccattg aaaccagtac aggcattctt tctgtacccg ttcccgttgg caaattcctg
    20461 ttgattaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
    20521 tgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
    20581 catcagtaag cgcaaaggaa ttgagcggat gtatgatttt ttgattattg gcggcggcat
    20641 catcggcatg tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt
    20701 gctggaaaaa gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca
    20761 tgccggagtc tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg
    20821 tgccactaaa gtcttttgcg atcaaaacgg cattcgctac gacaactgcg gcaagatgct
    20881 ggtcgccacc tctgaactcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc
    20941 gaacggtatc gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat
    21001 caccgggctt ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtgac
    21061 ggcggcgatg gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt
    21121 cagcgccctc agtgagcata aaaacggcgt ggtgatacgt acccgtcagg gcggtgaata
    21181 tgaagcatcg acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct
    21241 cggcctcgaa ccgggcttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc
    21301 ggagcataac cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt
    21361 tttgggcgtg catctcaccc gcatgatcga tggcagcgtg accgtcgggc caaacgcggt
    21421 gctggctttt aaacgcgaag gctatcgcaa gcgcgatttc tcgtttagtg acacgctgga
    21481 gattttaggc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggattggg
    21541 cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc
    21601 ccggctttcg ttaagcgatc tccagccctg gcccgcaggt gtgcgggcgc aggcggtatc
    21661 gccggacggc aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac
    21721 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
    21781 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg
    21841 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
    21901 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
    21961 acgccaacaa tggcgaggtc atcgacgtca ccaatccggc gaacggcgac aagctgggta
    22021 gcgtacccaa aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgctc
    22081 tgcccgcctg gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca
    22141 atttgatgat ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac
    22201 cactggctga agcgaaaggc gaaatcagct acgccgcctc atttattgag tggtttgccg
    22261 aagaaggcaa acgtatttat ggcgacacca ttcccggtca tcaggccgat aaacgcctga
    22321 ttgttatcaa gcagccgatt ggcgtcaccg cggctatcac gccgtggaac ttcccggcgg
    22381 cgatgattac ccgtaaagca ggtccggcgc tggcggcagg ctgcacgatg gtgctgaagc
    22441 ccgccagtca gacgccgttc tctgcgctgg cgctggcaga actggcgatt cgcgcgggcg
    22501 ttccgactgg cgtgtttaac gtggtcaccg gttcggcggg cgcggtcggt aacgaactga
    22561 ccagcaaccc gctggtgcgc aagctgtcgt ttaccggctc gaccgaaatt ggccgtcagt
    22621 taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagttgggc ggcaacgcac
    22681 cgtttattgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga
    22741 aattccgtaa cgccgggcaa acctgcgtct gcgccaaccg cctgtatgtg cagaacggcg
    22801 tgtatgaccg ctttgccgaa aaattgcagc aggcggtgag caaactgcac atcggcaacg
    22861 ggctggagaa aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg
    22921 aagagcatat tgccgatgcg ctggagaaag gcgcgcgcgt ggtttgcggc ggtaaagcac
    22981 atgaacgcgg cggtaacttc ttccagccga ccattctggt ggacgttccg gctaacgcca
    23041 aagtgtcgaa agaagagacg tttggccccc tcgccccgct gttccgcttt aaagatgaag
    23101 ccgatgtgat tgcgcaagcc aatgacaccg aatttggact tgcagcctat ttctacgccc
    23161 gtgatttaag ccgcgtcttc cgcgtgggcg aagcactgga gtacggcatc atcggcatca
    23221 acaccggcat tatttccaat gaagtggccc cgtttggcgg cattaaagcc tcgggtctgg
    23281 gtcgtgaagg ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg
    23341 gtctttaact ggagaatgcg aatgagcagc aataaagagt taatgcagcg ccgcagtcag
    23401 gcgattcccc gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc
    23461 cgggtgtggg acgttgaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc
    23521 aataccgggc acctgcatcc gaaagtggtt gcggcggtgg aagcgcagtt gaaaaaactg
    23581 tcgcacacct gcttccaggt gctggcttac gagccgtatc ttgagctgtg cgagattatg
    23641 aatcagaagg tgccgggcga ttttgccaag aaaactctgc tggttacgac cggttccgaa
    23701 gcggtggaaa atgcggtgaa aatcgcccgc gccgccacta aacgtagcgg tactatcgcc
    23761 tttagcggtg cgtatcacgg gcgcacgcat tacacactgg cgctgaccgg caaggtgaat
    23821 ccatactctg cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc
    23881 ccgctgcacg gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat
    23941 gacgccgcgc cggaagatat cgccgccatc gtgattgagc ccgttcaggg cgaaggcggt
    24001 ttctacgccg caacaccagc ctttatgctg cgtttacgcg cgctgtgtga cgaacacggg
    24061 atcatgctga ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg
    24121 atggagcaga tgggcgtggc gccggatctc accacctttg cgaaatcgat cgcaggcggc
    24181 ttcccgctgg cgggcgtcac cgggcgcgcg gaagtgatgg acgccgtcgc accgggcgga
    24241 ctgggtggca cctatgccgg taacccgatt gcctgcgtag cggcgctgga agtgttgaag
    24301 gtgttcgagc aggaaaatct gctgcaaaaa gccaacgttc tggggcagaa gctgaaagac
    24361 ggattgctgg cgatcgccga aaaacacccg gagatcggcg acgtacgcgg gctgggggcg
    24421 atgatcgcca tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc
    24481 gccgagatcg tggcacgcgc ccgcgataag ggtctgattc ttctctcctg cggcccgtat
    24541 tacaacgtgc tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt
    24601 ctggagatca tcagccagtg ttttgctgag gcgaagcagt agcaccgcac catgccggag
    24661 gcgacgctgc gcgtcttgtc cggcctacga gaatccaggt cggataaggc gtttacgccg
    24721 catccgacaa tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg
    24781 ctctccggca aaaagaacca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg
    24841 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
    24901 atgagttagg cggcgggctg aagtcacgcc acgtcaccat gttgtctatt gccggtgtta
    24961 tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat
    25021 tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa
    25081 tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgataaa gccattggcc
    25141 gctgggcggg ctataccatc ggctggttgt actggtggtt ttgggtactg gttatcccac
    25201 ttgaagccaa catcgccgcc atgatcctgc actcgtgggt tccaggcatt cccatctggt
    25261 tattttccct cgtcattacc ctcgccttaa ccggcagtaa tttattaagc gttaaaaact
    25321 acggcgaatt tgagttctgg ctggcgctgt gtaaagtcat cgctatcctg gcctttattt
    25381 tccttggtgc agtcgcaatt agcggttttt acccgtatgc cgacgtgagc ggtatttcaa
    25441 gattgtggga tagcggcggc tttatgccca acggcttcgg tgcggtatta agcgcgatgt
    25501 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg
    25561 acacgccgga aaaacatatt gtccgcgcca ccaactcggt tatctggcgt atctctattt
    25621 tctatttgtg ttctattttt gtcgttgttg cattaatccc gtggaatatg ccagggctga
    25681 aagccgttgg ttcttatcgc tcggttctgg aattgctcaa tattcctcat gcgaaattaa
    25741 tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggca ctgtataccg
    25801 cttccaggat gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa
    25861 tcaaccgcag taaaacccca tacgtagcgg tgttactctc caccggcgcg gcattcttaa
    25921 cggtggtggt gaactattac gcgcctgcga aggtgtttaa atttctgatc gacagctccg
    25981 gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acaattgcgg atgcgcaaaa
    26041 ttctgcgagc agaaggaagc gaaattcgcc tgcggatgtg gctttatccg tggctcacct
    26101 ggctggtaat cggttttatt acctttgtgt tggtagtgat gctattccgc ccggcgcaac
    26161 agttagaagt gctctccacc ggcttattag cgattgggat tatctgtacc gtgccaatta
    26221 tggctcgctg gaaaaagctg ataatgtggc aaaaaacacc cattcataat acgcgctgat
    26281 aatgatcagg agtcacacca tgaccattac ttctctggat ggctatcgct ggctgaagaa
    26341 cgatattatt cgcggcaatt ttcagccgga tgaaaaatta cggatgagtt tgctgacatc
    26401 gcgttatgca cttggcgttg ggccgttgcg ggaagcgctt tcgcaactgg tggcagaacg
    26461 actggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaagagct
    26521 gctcgatatt ttcgatgccc gcgccaatat ggaagcgatg ttggtgaatc tggcgattgc
    26581 ccgcggtggc gatgaatggg aggcagacgt tcttgcgaag gcgcatctgc tcagtaagct
    26641 tgaggcctgt gacgccagtg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
    26701 tcatactgca attgtggcgg gctgtggttc ttactatttg ctgcaaatgc gtgaacggtt
    26761 gtttgatctg gcagcgcgtt atcgatttat ctggctgcgg cgaacagtgc tttcggtgga
    26821 aatgctggaa gataaacacg atcagcacca gaccctgact gcgacggtac tggcgcgaga
    26881 taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
    26941 gcaggcgatg gctggcaatt aactactctt caggaatacg caacacttgc cccgggtaaa
    27001 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag
    27061 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
    27121 actggctggc agtggctggt gtcgccgttt tcacctgatc atcgacactg gcaataccgg
    27181 aaatattccc caccgcaata aggattttct ccttcgcctc ctgacttaag ccgtcaccag
    27241 tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
    27301 ttttgttcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
    27361 ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct
    27421 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
    27481 ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    27541 atagccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    27601 cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    27661 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
    27721 ttagcggagc ctgccagttt cagcgtacgc ttttactctc atttacgcgg ctgaggctgg
    27781 tatatgttgc actcttagtt tagagtttac ttaatttaga aaatacttaa ataactatga
    27841 ctgaactcgc acaattacag gccagtgccg aacaggcagc ggccttattg aaatcaatga
    27901 gccaccctaa acggttgctg attctgtgta tgcttagcgg ttcccccggc accagcgcgg
    27961 gagagctgac gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgctcgta
    28021 tgcgggacga agggcttatc gacagccaac gggatgccca acgcattcta tattccatta
    28081 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
    28141 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
    28201 caaagttaat cgatattcgt gatgctgatg aatatcttcg tgaacatatt cctgaagcag
    28261 atctagctcc attatccgta ctggaacagt caggtcttcc gcctaaatta cgtcgcgagc
    28321 aaattatatt ccactgccag tcaggtaaac gcaccagtaa taacgccgat aaattagcag
    28381 cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag
    28441 caggattgcc agtagcggta aataaatccc aatccttgcc gttaatgcgc caggtgcaga
    28501 tcgccgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcgttt
    28561 tcttcttatt aagcggcttt gttggtgctg ggttactgtt tgcaggaatt agtggttttt
    28621 gtggaatggc aaggttgtta gataagatgc cgtggaacca acgagcttga gaagcgacgc
    28681 cggacgcgcc atagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga
    28741 tttaccttct gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt
    28801 tttagtttca ccgttaacat cgatgaattt atatttcgcc ggacgcggct ggcgtttttt
    28861 accagcgcgt ggtgccgcag cagagctatt acccaataac tcttccgggt taattccgtc
    28921 agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg
    28981 ctgttgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
    29041 ttcaagaacg tcaatggaga attcgcgagc catcgcacga agggtgcgaa tgttatttaa
    29101 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt
    29161 tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt
    29221 cctgcacgag attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat
    29281 atattagcca ttttgatgag taatttcggt tattggtgaa cagagctgct ctctctgctc
    29341 gataattccg caagcctgac aaaaccaaag tgaataaaaa tgcaactcaa ccgaataaaa
    29401 gaaaacatct atcactggtg ctttctgctg tcaatgtaca ttatctggca taaaaaatgc
    29461 tgaaaatgac ccggtcacta catacaccga attctctacg cttatagcgt tacctcaccc
    29521 ccaaaccttc atgagtgaaa ataaaggtaa acacatatcc aattgattta attaaaaata
    29581 aatcataccg gcatgtaaaa atccacaatg tacaaaaaac gcgccgttac ggaatatttt
    29641 atctacaaaa acagattaaa taaaaatttt tcactaattg attagtcata gccagcgata
    29701 tacgctatgc gaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc
    29761 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
    29821 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
    29881 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
    29941 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
    30001 caaagttagc ccgtcgggct ggataaaaaa tctggcggat atcctggctt atgtgacgtt
    30061 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
    30121 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
    30181 cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggta
//
LOCUS       DAZFRE010000060        30027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFRE010000060 DAZFRE010000000
VERSION     DAZFRE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.060"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="ACHKUI_003020"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="ACHKUI_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6599180.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ACHKUI_003021"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ACHKUI_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6599181.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ACHKUI_003022"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ACHKUI_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6599182.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ACHKUI_003023"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ACHKUI_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6599183.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ACHKUI_003024"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ACHKUI_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6599184.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHKUI_003025"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHKUI_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6599185.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ACHKUI_003026"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ACHKUI_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6599186.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHKUI_003027"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHKUI_003027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6599187.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHKUI_003028"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHKUI_003028"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6599188.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ACHKUI_003029"
     CDS             complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ACHKUI_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6599189.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYTELKQLGLDGVMVYQETYNEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ACHKUI_003030"
     CDS             complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ACHKUI_003030"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6599190.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ACHKUI_003031"
     CDS             complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ACHKUI_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6599191.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ACHKUI_003032"
     CDS             complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ACHKUI_003032"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6599192.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRANGCPVCGGCNADPV"
     gene            complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ACHKUI_003033"
     CDS             complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ACHKUI_003033"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6599193.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAVDWRLATAQLLEIAGAGDE"
     gene            complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ACHKUI_003034"
     CDS             complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ACHKUI_003034"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6599194.1"
                     /translation="MSATKLTRREQRALAQHFIDTLEGSAFPNSKRIYITATQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGIITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18154..18254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ACHKUI_003035"
     CDS             complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ACHKUI_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6599195.1"
                     /translation="MLNQLDNLAERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18877..19650
                     /gene="nudC"
                     /locus_tag="ACHKUI_003036"
     CDS             18877..19650
                     /gene="nudC"
                     /locus_tag="ACHKUI_003036"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6599196.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19690..20754
                     /gene="hemE"
                     /locus_tag="ACHKUI_003037"
     CDS             19690..20754
                     /gene="hemE"
                     /locus_tag="ACHKUI_003037"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6599197.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVL
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20764..21435
                     /gene="nfi"
                     /locus_tag="ACHKUI_003038"
     CDS             20764..21435
                     /gene="nfi"
                     /locus_tag="ACHKUI_003038"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6599198.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21478..22068
                     /gene="yjaG"
                     /locus_tag="ACHKUI_003039"
     CDS             21478..22068
                     /gene="yjaG"
                     /locus_tag="ACHKUI_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6599199.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22255..22527
                     /gene="hupA"
                     /locus_tag="ACHKUI_003040"
     CDS             22255..22527
                     /gene="hupA"
                     /locus_tag="ACHKUI_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6599200.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22540..23235
                     /gene="yjaH"
                     /locus_tag="ACHKUI_003041"
     CDS             22540..23235
                     /gene="yjaH"
                     /locus_tag="ACHKUI_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6599201.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ACHKUI_003042"
     CDS             complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ACHKUI_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6599202.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINALAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            23894..25270
                     /gene="zraS"
                     /locus_tag="ACHKUI_003043"
     CDS             23894..25270
                     /gene="zraS"
                     /locus_tag="ACHKUI_003043"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6599203.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMMGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25267..26592
                     /gene="zraR"
                     /locus_tag="ACHKUI_003044"
     CDS             25267..26592
                     /gene="zraR"
                     /locus_tag="ACHKUI_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6599204.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ACHKUI_003045"
     CDS             complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ACHKUI_003045"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6599205.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDISALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLAGNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ACHKUI_003046"
     CDS             complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ACHKUI_003046"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6599206.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAVSNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLICGQWGERAPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7777 c         8277 g         6943 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagaa ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aacgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgac cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttatccc
     2641 cacgtatgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag atgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg ggtctttttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaggtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact acctgcgaca atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagttg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgcattactg cgcgccatat tcgccagctg gaaaaagacg
     4981 acgtcaagct gatcgaagtt ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 acattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcata agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagctg
     5281 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ctatgacttg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagttgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actccggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgcggcg
     6481 acgtgctggc agacggtccg tccaccgatc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     6661 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 ctctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgatg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aaactcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 atggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggcc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg tgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg cggtatgacc
     8701 aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggtgcg gaagcaatcc aggctctact gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga catcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gttgacgccc tgttggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gcgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtga tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtagcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggttt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta ctcatgaagg tatcatgatg actccggtta tcgaaggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat taacaaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgacaggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tatgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    12541 cttcacggaa tttttaagaa aggcagggcg aaacgaggaa gaagcttttc gccctggaca
    12601 acattcctgt tgacgggact accataaaaa aacacaacgc tttcaacaac cttgcgtttt
    12661 tcatcttccg gagctgctcg gaataaattt acgttcttac attctgtctc atggcctttg
    12721 cgatgggcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac cctgagcggt
    12781 taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact gttccagctc
    12841 ggggtggttg tcggcatagc cacctggctg cgttttcgaa aaagcgctga cgttattaat
    12901 tgccagcgga attacgcgat cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc
    12961 aatctccggt gcaagcagcc ggaaagcgca gatggcttgc actaactggc gttcatccat
    13021 aatcgacgca ggttcaatgc cgccagtaca tggacgcagg cgtgggaagg agacagagta
    13081 gcggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca tatagcagtc
    13141 aactcgccag ctgtcggaaa ggccgattag cgcgccgagg cctatcttat caatccccgc
    13201 acgccctagc cgatccggcg tttccagccg ccagaagaag tcctgttttt tacctttcag
    13261 atgatgatgg gcataagtcg cctcattata tgtctcctga taaaccatta ctccatcgag
    13321 gccaagctgt tttaactcgg tatattccgc ctccgccagc ggttgcactt ccatctgtag
    13381 tgaagagaac tgttcacgca gggcagggag atgacgacga aagtaatcca tccccacttt
    13441 cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc gtatagcggc
    13501 gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcggt tgctcataga
    13561 aaatccgcaa taagtacagt cgttagcgca aagattggaa agataaagcg ggacgtagaa
    13621 gctgacgacg ttgccaaatc gctgacgagt cagacgttgc gctcgctggg ccagtggttc
    13681 cagatagcca ctggcggcgg gtgatagcaa tgccatcatg tcgtcgcggg tgagttgcga
    13741 ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca ggtggatgtc
    13801 atcccagtcc agttgtcgcc agcgatcgct gaaggttttc atgccgatgc ctccagaaat
    13861 ccagtcagcg ggctggtggc atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt
    13921 gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac gggatcgtcc
    13981 gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag cgcctgcgcg
    14041 gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaacggtggc ctgctggata
    14101 ataatctcca gcatggcgcg ggtttccagt ccctgattcg agccaatcgg cgcgccgagc
    14161 ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg atcggctccg
    14221 cagtaaggca gcacgataaa tccctgttgt accagcgttt cggcggcttt cagggtttcg
    14281 atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag ccagtttgtg
    14341 cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgcggt tttcgccccg
    14401 gaagtattcg gcagcagggt cacacccgcc gcgataagcg gttcgaggat agcgtcgttg
    14461 tgctggcgca agtcgacacg tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga
    14521 atagactcca ccatcagttg tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa
    14581 tcaaacgttt tgtccgcaat atgtaacatc tcaaccccct gcaataactt gaaaaagcag
    14641 gatctggtcg ccatcctgca cgatatgttg cgcccattgt tcccgcggga cgatttgctg
    14701 attaatcgct agtgccgcac ctgcttgttg ttgatcgagt tgctccagca gttcgtgaac
    14761 agttagtccg gcgacacact gcatcggttg atcgttaaac aggatctgca ttgcatcctc
    14821 cacataccgg gcaaccgttg gcgcggcgca acgtcaggct gcgccactgg ctcgatttac
    14881 cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac ttaatcgctt
    14941 ccagtgcctg taaagtgccc ataaccccga ccaccgggcc aaccacgccc gccgtgcggc
    15001 agttgcgttc tggctcctgg ttatctggcc acaggcagcg gtaacatccc tgctcccagg
    15061 gcggcgtcag taccatcaac tgcccgccaa atccgaccgc gctggcggtg ataagcggcg
    15121 tgttgagtgc cacgcaggtg gcattaatct cctggcgagt cgccatattg tcagtacagt
    15181 cgagcaccac atcggcttgt gcaaccgcat cttttaacgc ctcacccgtt aaccgttgtt
    15241 gtagtgccgt cagttgaatg tcgggattca actgtgtcag tcgctgttgg ctgacctgtg
    15301 atttcgggcg atcgatatct tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat
    15361 gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccccgcc aggtatagcg
    15421 cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg agcagttttt
    15481 gctgcccgtc cagagcgata tcgtcgagca ggatttggcg gctataacgc ataaagtcac
    15541 ggtcattcat cgccagctcc tgcaatttcc agcaactgtg ccgttgccaa acgccagtct
    15601 acggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc tatcaccgca
    15661 ggcgcgcgcg ccagactgat accgccaatc gccacggtgg gataatccgc caggcgctca
    15721 acatgccgtg ccagctgttc cagtccctgc ggtgcagaag gcatctgttt ggtttgcgtt
    15781 gggaacacat gtcccagcgc gatataagaa ggacgtgccg ccagcgcgac gtcgatctcc
    15841 atatcgtcat gagtcgaaac gcccagccgc aggcccgctg cacggatggc attgagatcg
    15901 gtggcttgca aatcttcctg ccccagatgg acgccatacg cctgatgctt aatcgccagc
    15961 cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc
    16021 acgacatcgg cttccacctc ttcatcgcgc cgatctttga tgcgtagctg gagagtacgt
    16081 acgcctgcat ccaacagacg ttcgatccac tgtacgctgt caaccaccgg gtacagtcct
    16141 aaacgaaagg gtacaggagg aaactcaggc tgatacatca cgcttcctcc ttacgcaggt
    16201 agatttctcc gcctctggcg cggaagttct ccgacatatc cgccattccc acttcaatgg
    16261 tttgcgcggc ggcgtaatca cgcacttcct ggctgatttt catcgagcag aatttcggtc
    16321 cacacatgga gcaaaaatgg gcgactttac cggactcttg cggcagggtt tcatcgtgat
    16381 aagcgcgggc agtaaacggg tcgagggcca gattaaactg gtcttcccag cgaaattcga
    16441 agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt
    16501 cggcggcgtg agcggcaatc ttataggtga taatcccttg tttaacatct tctttatttg
    16561 gcaggcccag atgctctttc ggcgttacgt agcagagcat cgcgcagcca aaccagccaa
    16621 tcatcgccgc gccaatccct gacgtgaagt ggtcatagcc cggcgcgata tcggtagtta
    16681 gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc
    16741 ggcgaatcat ctgcatcggc acgtggcctg ggccttcaat catcacctgc acgtcatatt
    16801 cccaggcgat tttagtcagt tcacccagcg tatgcagctc ggcaaactgt gcttcatcgt
    16861 tggcgtcctg aatagaaccg gggcgcaggc cgtcgcccag cgatagcgaa acgtcataag
    16921 cagcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc tgatgatggg
    16981 agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc aggcgtttcg
    17041 cggtcatcgg cacgtagcgc agcagcaccc ccgcatggat agtgaagtaa tccacacctt
    17101 gctcggcctg ttccagcagg gtgtcgcgga acgcttccca ggtaagatct tcggcgatcc
    17161 cgttcacctt ctccagtgcc tggtagatcg gcactgtacc gatcggcacc gggctgttac
    17221 gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccagt ggaaagatcc atcacggtat
    17281 ccgctcccca gcgcgtggac cataccagtt tttccacttc ttcttcgatg gaagaggtga
    17341 ccgctgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca ataatcatcg
    17401 gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca gcaacttcat
    17461 cacggacaaa ttccgcagtg atattttccg gaagacgtgc gccaaagctc attcccggat
    17521 gctggtggcg taaaacttcg ctacgtatgc gctcgcgacc catattctcg cgaatggcga
    17581 tgaattccat ttcgggcgtg atgataccct ggcgggcgta gtgcagttgg gtgacgcggc
    17641 gtcctgcttt ggcgcgtttt ggcgttaata cgccgctaaa acgcagctcg tcgaggccat
    17701 catctgccag ccgcgctttt gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgc
    17761 cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg ttaatggcga
    17821 tctgcggatc gccatacgga ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt
    17881 actgcggttg ttctttgcta ccgccgatta gcgtcgggct aagctggatc tcacgcatcg
    17941 gcacgcgcac gccgggttgt gtggcagtga tgtaaatgcg ttttgagttg ggaaaggcgg
    18001 agccttccag ggtatcgata aaatgttggg ccagggcgcg ttgttcgcgg cgggtcagtt
    18061 ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga cgacggatga
    18121 agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt tcccttcgca
    18181 ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca gttaaggcac
    18241 tccgacaaga aattagcccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc
    18301 agaactcaag caggatattt gacgcgggcg gcgtcaagca ccagtagaat cagcttatct
    18361 tccagcacaa agcgcgcttc cagtgcctcg ccgatgtcag ataaaacctg ttgaaactca
    18421 aggtaattat catgatcgat ggcggtttcc agactggaat cgtagtcatc cataatctgt
    18481 tgagtattgg cttcgagttg tggccaaatc ttcgcggcgc gtgcgagttg cccattgcct
    18541 tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc
    18601 aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca
    18661 ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag
    18721 cgatcaacca gtttgttact tcctctgacg cgctccgcca ggttatcgag ctggttaagc
    18781 atgattgact ccgcaagttt gtattcaaaa actgctctgt gagaaatgta aaaaccatgt
    18841 taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta
    18901 gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg
    18961 ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa
    19021 tggcaggggg aacctgtttg gttagtacaa ctgcagcggc gtcacgatat ggggtcggta
    19081 cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg
    19141 gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc
    19201 aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc
    19261 ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc
    19321 catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggtga aaccctcgag
    19381 caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttac
    19441 gtgacctccc aaccgtggcc gtttcctcag tctttaatga ccgcgtttat ggcggaatat
    19501 gacagcggcg agatcgtgat cgacccgaaa gaattgcttg aggcgaactg gtatcgctat
    19561 gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat agaagataca
    19621 gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac gcactaagga
    19681 acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc
    19741 gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa
    19801 gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaatgccga gctggcgtgc
    19861 gaagtgactt tgcaaccgct gcgtcgctat ccgctggatg cggcgatcct cttttccgat
    19921 atcctcaccg tgccagacgc gatgggttta gggctctatt ttgaagccgg agaaggtccg
    19981 cgttttacct cgccagtcac ctgcaaagcc gatgtcgata aactgccaat tccggacccg
    20041 gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc
    20101 gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcgaccta catggtggaa
    20161 ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga tgtatgccga tccacaggcg
    20221 ctgcacgcgc tactcgataa actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt
    20281 aaagccggtg ctcaggcagt catgattttc gacacctggg gcggcgtgct taccgggcgc
    20341 gattatcaac agttctcgct ctattacatg cataaaattg ttgatggttt actgcgtgaa
    20401 aacgacggtc gccgcgtacc ggtcacgctg tttaccaaag gtggcggaca gtggctggaa
    20461 gcgatggcag aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat
    20521 gcgcgccgtc gcgtgggcaa taaagtcgcg ctgcagggta atatggaccc gtcgatgctg
    20581 tacgcgccac ctgcccgcat tgaagaagaa gtcgcgacta tacttgcagg tttcggtcac
    20641 ggtgaaggtc atgtctttaa ccttggtcac ggcattcatc aggatgtgct gccagaacat
    20701 gctggcgtgt ttgtggaggc agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg
    20761 attatggatc tcgcgtcatt acgcgctcaa caaattgaac tggcttcttc tgtgatccgc
    20821 gaggatcgac tcgataaaga tccgccggat ctgatcgccg gagccgatgt cggatttgag
    20881 cagggcggcg aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg
    20941 gtggagtata aagtggcccg catcgccacc accatgcctt acattccagg ttttctctcg
    21001 ttccgcgaat atcctgcgct gctggcagcg tgggagatgc tgtcgcaaaa gccggattta
    21061 gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtggc cagccatttt
    21121 ggcttattgg tggatgtgcc gaccattggc gtagcgaaaa aacggctctg cggtaaattc
    21181 gaaccgctct ccagcgaacc gggcgcgctg gccccgctga tggataaagg cgagcagctg
    21241 gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg
    21301 gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg
    21361 gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca
    21421 gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga actcaacatg
    21481 ttacaaaacc caattcatct gcgtctggag cgcctggaaa gctggcagca cgtcactttt
    21541 atggcctgct tatgcgaacg tatgtacccc aattacgccg tattctgtca gcaaaccggc
    21601 ttcggcgatg ggcagattta ccgccgcatt ctcgatctca tttgggaaac gctgaccgtc
    21661 aaagacgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca
    21721 gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcttgcgt ggcgttaagt
    21781 gaactggtcc attcgcgttt aagtggcgaa acgctcgaac acgcggtgga agtgagtaag
    21841 acttccatca cgaccgtggc gatgctggaa atgactcagg ctggtcgcga aatgagcgat
    21901 gaagagctca aagaaaaccc agctgtagag caagaatggg acattcagtg ggaaatattc
    21961 cgacttttag ctgagtgcga agaacgcgat atcgagctga taaaaggcct tagggcagac
    22021 ctgcgtgagg cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg
    22081 tgatttaacg cctgatttgt cgtacctgga gtcttccctt tcgccccccg tctggtctac
    22141 atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc
    22201 atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac
    22261 aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa
    22321 gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta
    22381 caactggttg gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac
    22441 ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc
    22501 aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg
    22561 gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcagg cgctctgttt
    22621 ctcactgcct gtagtcacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa
    22681 gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc
    22741 gtgtttagcc cgtggcgcag tggcgatacc accacgcgag agtatcgctg gcagggcgat
    22801 aaccttacgc tcatcaatat caatgtttac agcaaaccgc cggtgaacat tcgcgcgcgt
    22861 tttgacgatc gcggcgatct gagctttatg caacgtgaat ccgatgggga aaagcagcag
    22921 ctttctaacg accaaatcga tttataccgt tatcgtgctg atcagatccg ccagattagc
    22981 gatgccttac gtcaggggag agtcgtgctg cgccaggggc gctggcatgc gatggaacag
    23041 accgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg
    23101 catatcgagc gccgccagag ccgctcttct gttgatgtca gcgtggcatg gctggaagcg
    23161 cccgaaggtt cgcaattact gttggtggca aactctgatt tctgtcgctg gcaacccaac
    23221 gagaaaacgt tctgatttac cagtggccca tacccatatg accgccacca ccgcagtcgc
    23281 cgtaacccat tccggtaccg cgcggaatac ccgcttcagc catcgcaata tctcgtttca
    23341 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgagc gcgttaattt
    23401 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc tttgtcacca
    23461 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    23521 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccatgt ccgccgtgag
    23581 caaatgccga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    23641 tcgtgttccg tttcatggtt catcctccag tggttgtctt acttcgggta ttgcatcttc
    23701 cgtgccaacg gcgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    23761 actcgcctgt tgtgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    23821 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    23881 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    23941 cgcgatcctc cccgtggtca ttgttgggct ggtggggctg tttgcggtga cagtgattcg
    24001 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24061 tatccgtgct ctcgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24121 gcagcaggca ttactggaag aaatggccgg gcagcctggt gtacgttggt ttgcggtcac
    24181 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atgatgggaa aacagcttta
    24241 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    24301 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    24361 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caatttttat
    24421 cgctttcgac gccagtaaca ttgtgagtgc tgaagatcgt gagcagagaa acaccctgat
    24481 tatcctcttc gccctggcga cggtcttgct ggcaagcgtg ttgtcattct tctggtatcg
    24541 ccgatatctg cgctcgcggc agctattgca ggatgaaatg aagcgcaaag agaagctggt
    24601 ggcactgggg catctggcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    24661 taaagggctg gcgaaatact ttgccgaacg cgcgtcagca gggggagaag cgcatcaatt
    24721 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agttgctgga
    24781 actggttaag ccaacgcatc tggctttgca ggcggtggag ctcaacacgc tgattaacca
    24841 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    24901 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    24961 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25021 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcgga
    25081 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggactggg
    25141 gctggcggtt gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25201 ggagggaaaa ggcgcaacgt tcaccctttg gcttccggtc aatattacgc gtaaggaccc
    25261 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    25321 actattttgc aggctttgct gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    25381 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    25441 atggcagaga tggatggtat cgccacgcta aaagagatca aaacattaaa ccccgcgatt
    25501 ccggtgctga ttatgaccgc gtactccagc gtcgagacgg cggtagaggc gttgaaaacc
    25561 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    25621 aaggcgctcg cgcatacaca cagtgttgac gctgaaacgc ctgcggtgtc tgccagccag
    25681 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    25741 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    25801 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgc
    25861 gcggcgctca acgaatcctt gctggagtct gaattgttcg gtcacgaaaa aggggcgttt
    25921 accggggccg acaaacggcg ggaggggcgc tttgttgagg cggacggcgg cacgttgttt
    25981 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctacg tgcgattcag
    26041 gagcgcgaag ttcagcgtgt cggtagcaac cagactattt cggttgatgt ccggctgatt
    26101 gcggcgactc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26161 tactatcgcc tgaacgtggt ggcgattgaa gttccgtcgc tgcgtcagcg gcgggaagat
    26221 attcctctgc tggcaaacca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    26281 aaaggtttta cgccccaggc gatggatctg ctgattcact acgactggcc gggaaatatt
    26341 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    26401 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggtaca aagtcaggat
    26461 attcagccgc tggtggaagt cgaaaaagag gtgattcttg cggcactgga gaaaacgggc
    26521 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct cttggcaaaa
    26581 ctgtcgcgtt agttctgctc gcgttcgata gcgcgccagc cgatatcttt ccggcagaag
    26641 cagtcgtccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgctacg
    26701 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggtcactac ctgctcgtta
    26761 cccgccagtt tcgtgcccgc gtggaacact ttgccgtcag acacttcttc cagcggcagg
    26821 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    26881 ccgagagaag cgcgttcatc ccactcggac gttttctcgt ccagcttgcc ttcacaggcc
    26941 gccaggcaga gttcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27001 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27061 cccgcgtaga gaaaaccagt gtaggtgttg ccttctgccg ccatgccttt cacggttggc
    27121 cagatgattc gttccatcgt gcgctgatga acctcatcgg tcactactgg cgcaggggag
    27181 taagcgccca tcccgccggt gttcggcccg gtatctttat cgcctacgcg tttgtgatcc
    27241 tggctggtgg ccatcggcag cacatgctcg ccgtctacca tcacgataaa gctcgcttct
    27301 tcgccgtcga ggaactcttc gataacaatg cgatgacccg cgtcgccaaa cgcgttgccc
    27361 gccagcatat cgcgcacggc ggcttcagct tcttccagcg tcatcgccac gataacgcct
    27421 ttcccggcgg ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    27481 gccagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat tttatggcgg
    27541 gccaggaaat ctttggtaaa cgctttcgag ccttccagtt gcgcggcacc tgcggttggg
    27601 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    27661 tccgggccga cgatggttag atctaccttt tcgttttgtg cgaaatccag cagcgccgag
    27721 atatcggtta cgccgatagc gacgttctgt agggcgggtt ccagcgcagt gcctgcgtta
    27781 cccggagcaa caaaaacagt ttcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    27841 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    27901 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    27961 cgtcatcacg gatagaaccg ccaggctgga ttacacaggt cacacccgca gcggcggcgg
    28021 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28081 ccagaccttc atcggcggct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28141 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagcg ttggatttca
    28201 cgaacttcgc cactttccag cagaacagcg catcacgcaa ttcctgttcg gtcggctggc
    28261 gtttggtgac gacgcgcagt tcttctgcac cgaccatccc caggtcgcga tcctgaacca
    28321 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggagc acgctcgccc cactgaccgc
    28381 agatcaggac gcgaacgttc tgtttagcgg cggttatttt cagggcttct tcgctggcgg
    28441 acggcgcaat aatcacttca acaaactgac gagaaatgat ggcctgcgcg gtttccgcat
    28501 ccagctcgcg gttaaaggcg ataatgccgc cgaatgcgga ggttgggtcg gttttatacg
    28561 cgcgatcgta agcatcgaga atggagttgc tgaccgccac gccgcatggg tttgcatgct
    28621 tcacgatcac acaggccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    28681 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggtg gcaacggagg
    28741 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtagc
    28801 gcatatcctg cttcttgatg aagttcaggt tcagcgtacg cgggaagcga ccggcggctt
    28861 ctttgctttc accgtgatag gccggaacca tgctgccgaa gtagttggca atcatgctgt
    28921 cgtaggcggc agtgtgttcg aaggctttga tggcaaggtc gaaacgggtt gcaagcgtca
    28981 gcgatccttc gttggcatcc atctctttaa taatggcgtc gtagtcgcta ctcttcacca
    29041 cgattgcgac atctttatgg ttcttggcgg cggagcgcac catcgttggg ccgccgatat
    29101 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcgacggtc tgggcgaacg
    29161 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29221 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    29281 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    29341 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    29401 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    29461 ctggacgacg ttgttgcatg gtaaatcccc tggatttgac tattacagag agcgttagct
    29521 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    29581 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    29641 ttttgacagt ggctaccgtg ggaaaaatac agaaattaca ttgaagattg tggataactc
    29701 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcattttta
    29761 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgcctcca tcgacacggc
    29821 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    29881 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg agctgaaagc
    29941 cgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    30001 gattcttgac gtcgcaagac gaaaaat
//
LOCUS       DAZFRE010000061        29667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFRE010000061 DAZFRE010000000
VERSION     DAZFRE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.061"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..188)
                     /locus_tag="ACHKUI_003047"
     CDS             complement(<1..188)
                     /locus_tag="ACHKUI_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6599207.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGF"
     gene            350..523
                     /locus_tag="ACHKUI_003048"
                     /pseudo
     CDS             350..523
                     /locus_tag="ACHKUI_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(755..1618)
                     /gene="potB"
                     /locus_tag="ACHKUI_003049"
     CDS             complement(755..1618)
                     /gene="potB"
                     /locus_tag="ACHKUI_003049"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6599208.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPGAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(1602..2738)
                     /gene="potA"
                     /locus_tag="ACHKUI_003050"
     CDS             complement(1602..2738)
                     /gene="potA"
                     /locus_tag="ACHKUI_003050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6599209.1"
                     /translation="MGQSKKLNKQPNSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2988..4214
                     /gene="pepT"
                     /locus_tag="ACHKUI_003051"
     CDS             2988..4214
                     /gene="pepT"
                     /locus_tag="ACHKUI_003051"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6599210.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(4263..5384)
                     /gene="roxA"
                     /locus_tag="ACHKUI_003052"
                     /gene_synonym="ycfD"
     CDS             complement(4263..5384)
                     /gene="roxA"
                     /locus_tag="ACHKUI_003052"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6599211.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(5460..6920)
                     /gene="phoQ"
                     /locus_tag="ACHKUI_003053"
     CDS             complement(5460..6920)
                     /gene="phoQ"
                     /locus_tag="ACHKUI_003053"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6599212.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(6920..7591)
                     /gene="phoP"
                     /locus_tag="ACHKUI_003054"
     CDS             complement(6920..7591)
                     /gene="phoP"
                     /locus_tag="ACHKUI_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6599213.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(7761..9131)
                     /gene="purB"
                     /locus_tag="ACHKUI_003055"
     CDS             complement(7761..9131)
                     /gene="purB"
                     /locus_tag="ACHKUI_003055"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6599214.1"
                     /translation="MELSSLTAVSPVDGRYCDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(9135..9776)
                     /gene="hflD"
                     /locus_tag="ACHKUI_003056"
     CDS             complement(9135..9776)
                     /gene="hflD"
                     /locus_tag="ACHKUI_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6599215.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(9812..10918)
                     /gene="mnmA"
                     /locus_tag="ACHKUI_003057"
                     /gene_synonym="trmU"
     CDS             complement(9812..10918)
                     /gene="mnmA"
                     /locus_tag="ACHKUI_003057"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6599216.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(10972..11433)
                     /gene="nudJ"
                     /locus_tag="ACHKUI_003058"
     CDS             complement(10972..11433)
                     /gene="nudJ"
                     /locus_tag="ACHKUI_003058"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6599217.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(11443..12096)
                     /gene="rluE"
                     /locus_tag="ACHKUI_003059"
     CDS             complement(11443..12096)
                     /gene="rluE"
                     /locus_tag="ACHKUI_003059"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6599218.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWREATD"
     gene            12268..13518
                     /gene="icd"
                     /locus_tag="ACHKUI_003060"
     CDS             12268..13518
                     /gene="icd"
                     /locus_tag="ACHKUI_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6599219.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            13955..14725
                     /locus_tag="ACHKUI_003061"
     CDS             13955..14725
                     /locus_tag="ACHKUI_003061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599220.1"
                     /translation="MDIIIVPRDQRIPASGISTAYLHVDHWNDFSFITMFYMYLFDQK
                     GERHEIGNVKIGFQGQTTQQSTYSTLGDRFKILPEGYFSVGQDVDYYQRISNLPESVK
                     VSLLEALKDIAYTPELIDFVKNEAVFKTSLLRYVSLSVIKGQFARVLEGKSPLTNFEF
                     KFIRPAQDKISDIELSFKVKVGEKPSTNIHAIIGRNGVGKTTILNGMIEAVTSKGTSN
                     AKFYDLEGWREDPIDNDYFSSLVSVSFSAFDPFEPPSE"
     gene            14765..15472
                     /locus_tag="ACHKUI_003062"
     CDS             14765..15472
                     /locus_tag="ACHKUI_003062"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6599221.1"
                     /translation="MKKKGESLKGLNDIHEDFLEALTLCFSQHPKRERWLKAIDTLES
                     DENFASMELKNLAEYNDEMLVSNARKCIKRMSSGHAVVLLTLTRLVATVEEKTLVLID
                     EPESHLHPPLLSAFIRALSDLLLDRNGLSIIATHSPVVLQEVPKRCVWKINRSRLSTE
                     SRRPDIETFGENVGILTREVFGLEVVKSGFHDLLVQSVKSGGTYEEIVDEYRNQLGVE
                     ARALLKALVTHRDRSVK"
     gene            15472..16329
                     /locus_tag="ACHKUI_003063"
     CDS             15472..16329
                     /locus_tag="ACHKUI_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6599222.1"
                     /translation="MLKLKQPAYTYADMLTSCREGITGNPGLLLKVNEAITDLQTLAA
                     SYSASASTGELYTIQPLKNKDKEDPIVVGRLKKFDFVKLYGNYVVGKKKPARAIYDSL
                     ITAANEKCPYCGGIGRPRNLDHYLPKAYYPQFSILPVNLVPACRDCNMDGKGEVFARN
                     EAEQIIHPYLDDSRYYDEQWISARYLAGKNDEPGVIEYFVSPPADWDEAHKQRVEKHF
                     NDFDLSLRYSKEASARLVVLLALYNSVLPLSRDKNAAKHLIFQTIINKSPFVNHWERV
                     MCLALMSDL"
     gene            complement(16598..17724)
                     /gene="intE"
                     /locus_tag="ACHKUI_003064"
                     /pseudo
     CDS             complement(16598..17724)
                     /gene="intE"
                     /locus_tag="ACHKUI_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(17705..17842)
                     /locus_tag="ACHKUI_003065"
                     /pseudo
     CDS             complement(17705..17842)
                     /locus_tag="ACHKUI_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003841900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            17917..18726
                     /locus_tag="ACHKUI_003066"
                     /pseudo
     CDS             17917..18726
                     /locus_tag="ACHKUI_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            19053..20129
                     /locus_tag="ACHKUI_003067"
     CDS             19053..20129
                     /locus_tag="ACHKUI_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HFO6599223.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTIRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLDVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSRIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQLNSIPITA"
     gene            20146..20454
                     /locus_tag="ACHKUI_003068"
                     /pseudo
     CDS             20146..20454
                     /locus_tag="ACHKUI_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            20476..21363
                     /locus_tag="ACHKUI_003069"
                     /pseudo
     CDS             20476..21363
                     /locus_tag="ACHKUI_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774771.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            21360..22061
                     /locus_tag="ACHKUI_003070"
     CDS             21360..22061
                     /locus_tag="ACHKUI_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6599224.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTSL
                     YQNMRANALTDTELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGVMK"
     gene            22311..26219
                     /locus_tag="ACHKUI_003071"
     CDS             22311..26219
                     /locus_tag="ACHKUI_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6599225.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQTYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNVHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVNNSGVSTADSSFT
                     LDGDSSPTISADGQSTYPIVLSLKDSNGKALTGLADDIEMSVEFTADSNSARQRETVT
                     ALSLGAVEEISAGVYRSVLAAGSQAGTVRVTAKVQGKTFTLNIKQAAVIDSDVSTADS
                     SFTLDGDSSPTISADGQSTYPIVLSLKDSNGKALTGLADNIEMSVEFTADSNSARQRE
                     TVTAPSLGAVEEISAGVYRSVLAAGSQAGTVRVTAKVQGKTFTLSIKQTAATEPDSEV
                     SAVLTAAPAEQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDG
                     VYSATVTSKRAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGK
                     QRNMVTVSLADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGS
                     YVITAHAGSQMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNT
                     NASVNGQKIQLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVE
                     SGSVKLTFVPDVASAELTMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITP
                     STGASLTSSNLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKG
                     AVLEITSSASSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTT
                     VTTDENGQATVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPG
                     YETNDGSPVGIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAA
                     SEFSTYESGYHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
     gene            complement(26256..27299)
                     /locus_tag="ACHKUI_003072"
     CDS             complement(26256..27299)
                     /locus_tag="ACHKUI_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFO6599226.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            27649..27750
                     /locus_tag="ACHKUI_003073"
     CDS             27649..27750
                     /locus_tag="ACHKUI_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599227.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            27747..28202
                     /gene="ybcN"
                     /locus_tag="ACHKUI_003074"
     CDS             27747..28202
                     /gene="ybcN"
                     /locus_tag="ACHKUI_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6599228.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            28202..28372
                     /gene="ninE"
                     /locus_tag="ACHKUI_003075"
     CDS             28202..28372
                     /gene="ninE"
                     /locus_tag="ACHKUI_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6599229.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKCKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            28365..28655
                     /gene="ybcO"
                     /locus_tag="ACHKUI_003076"
     CDS             28365..28655
                     /gene="ybcO"
                     /locus_tag="ACHKUI_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6599230.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            28652..29014
                     /gene="rusA"
                     /locus_tag="ACHKUI_003077"
     CDS             28652..29014
                     /gene="rusA"
                     /locus_tag="ACHKUI_003077"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6599231.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYCDNVARIIKN
                     AMLDIGLAMPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            29011..29151
                     /locus_tag="ACHKUI_003078"
     CDS             29011..29151
                     /locus_tag="ACHKUI_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6599232.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            29237..29620
                     /gene="quuD"
                     /locus_tag="ACHKUI_003079"
     CDS             29237..29620
                     /gene="quuD"
                     /locus_tag="ACHKUI_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO6599233.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
BASE COUNT         7835 a         6753 c         7338 g         7741 t
ORIGIN      
        1 taaaacccag ttccaacgcc aatatccaga tggttgttac ctaaatgttc cagaaagtgt
       61 ggaagaaggt gttcctttgt aggacatccc catgcaagcc gatttgatac tcccaaaacc
      121 caccagtcat aaagctttag ggtaagtggt gtgtaaattt tagccccatc atctgtgttt
      181 tttttcatta gtttcaccgt attatagttt tatttgtgaa ttaaatcaat tatggcgatg
      241 aattacaagg ggttaaatgc tgccgcagca tagcgatatt gaaatagcct ggtatgcttc
      301 gatacagcag gagccgaatg gctggaagac cgtcaccaca cagttctaca tccaggaatt
      361 cagtgagtat attgcgccac tgcaggatgc tgtaggtctg gaaatcgcaa cggaggaaga
      421 aagatcgttg ctggaggcat ggaataaata tcgggtattg ttgaatcgtg ttgatacatc
      481 aactgcacct gatattgagt ggccgacttc acctgcagag taatcaacgg cgcagggatg
      541 atattttgtg cttgaagtgc ctccccgacc attcagatag tcggggaggg gtattgggat
      601 tatacgcaat cagttttagt taaagaccga agctctcgaa aaatctttct ccaaaaccag
      661 aatggaattg attgattttg ataggatcta taacgtcgtt accttaaacg gcaaaaccac
      721 tcaaaaacaa acttaatgca ttgaatataa aggattaatc atctaattcg ctcaccttct
      781 tattcagcag acgagaagcg cgccagtaaa ccagcaacat caggcccatt acgatagtca
      841 gcgtaatgct ggtggctgca ccaaacggcc agtcacgaat attgaggaac tgtaccttga
      901 tgacgttacc gatcagcagg tttttcgcac cgcccatcag gtcggacaca tagaacaggc
      961 ccatcgctgg cagcatcacc agcagacatc cggcaataat tcccggcatc gttagtggga
     1021 taatgatgcg gataaaggtc tgcaacttgc tggcaccgag atcgcgcgcc gcctcaagca
     1081 acggcttatc cagtttttcg atactggagt acagcggcat caccataaac ggcagcagaa
     1141 tgtaaaccag accgataatc accgcaccag gcgtgaacat gatgcgaatt ggtgtatcga
     1201 taacgcccag ccagagcaaa aactcgttga gatagccttt ggtgctgagg aaaattttca
     1261 gcccgtaaat acgaatcaat gagttggtcc agaacggaac aatcagcaga aacagcagca
     1321 gcggacgcac cttgtgtggc agcttcgcca gaaaccaggc aaaaggatag cccagcacca
     1381 ggcaggcgag ggtggcgatc agcgccatat tcagcgagtg caatagcact tcaaaataga
     1441 gcggatcgag cagacgcgtg tagttatcca gcgtaaagac cattttgacg aaactggcgt
     1501 cgtcgcgggt caaaaagctg gtgccaatga tcatcaggtt gggcagaaag acaaacaaca
     1561 caagccaacc gacaatagtg acaatcacta cattctggaa cttacttgtg ttcttcatca
     1621 gccagtacga cctcccagct ttctacccaa ttaatggcca ttttttggtc gagagagtgg
     1681 tcaaagtcag gatcgtcttc attgaagaat tcgctgacca tcaccatctt gccattttcc
     1741 agctcaacaa ccgactccag cgtcatgcct ttgtagttac gctcgcgaac gtaaccaatc
     1801 agcccttcgg cgtggttgtc gtcgttaatc tcttcaacac gtaagtcttc cgggcgcagc
     1861 agaacatgca gtttttgccc cggttcaacg gcgaagttaa cgtagatatt acattcgcgg
     1921 ccttcaacgt tggcgcgtac gcgctgctcg tcgagacgtt cgatgacagt ggcgttaaac
     1981 atattgattt cgccaataaa cccggcaaca aacaggtttt tcggctcttc gtagatttca
     2041 cgcggcgtgc cgtcttgctc aatgcgacca tcgcgcatca ccacgatcct gtccgacatg
     2101 gtaagtgctt cttcctggtc gtgagttacg aacacgaatg taatgccaag cttacgctgt
     2161 aacgctttca gctcgttctg catttgcttg cgcagtttgt aatccagcgc tgagagcgac
     2221 tcatccagca acaacagacg aggcttgtta accaccgcgc gagcaatggc gacgcgttgc
     2281 tgttgaccac cagagagctg atgcggtttg cgttgagcga aggtttccag ctgcaccatc
     2341 cgcagggctt ccatcacgcg gggcgtaatt tcagcagcgg gggttttttg catgcgcaac
     2401 ccaaaggcca cattttcgaa cacggtcatg tgggggaaaa gtgcgtagct ttggaaaaca
     2461 gtgttcacat agcggttttc cgccggaacg tgggtgatgt cctcgttatc cagcatgatg
     2521 cgtccggaat caacagtttc cagacctgca atcaggcgaa gaacggttgt tttaccgcag
     2581 ccagaagggc caagcagcgt gaggaactcg ccattgttga tagtcagatc cagctgggga
     2641 atgacctctt taccatcaaa gcatttgcga attcccgcca attgcaccag cggtgaaagc
     2701 gaattcggtt gtttattcaa ttttttactc tgtcccatgt aaacgcaacg gatggcttac
     2761 cgatgcgggg tttgtggtta accaccttgg tgactcttaa tgagggcggt aattctacgg
     2821 caaaccgctt gaatcgccaa tctttgttgt gaattactgg cttagcttta tattcattaa
     2881 ggtaatgctg ataaatattc ccgcttgcag ggctaaaagt gacctgacgc aatatttgtc
     2941 ttttcttgct tcttaataat gttgtcacaa aaagtgaggg tgactacatg gataaactac
     3001 ttgagcgatt tttgaactac gtgtctctgg atacccaatc aaaggcaggg gtgagacagg
     3061 ttcccagcac ggaaggccaa tggaagttat tgcatctgct gaaagagcag ctcgaagaga
     3121 tggggcttat caatgtgacc ttaagtgaga agggcacttt gatggcgacg ttaccggcta
     3181 acgtccctgg cgatatcccg gcgattggct ttatttctca tgtggatacc tcaccggatt
     3241 gcagcggcaa aaatgtgaat ccgcaaattg ttgaaaacta tcgcggtggc gatattgcgc
     3301 tgggtatcgg cgatgaagtt ttatcaccgg ttatgttccc ggtgctgcat cagctactgg
     3361 gtcagacgct gattaccacc gatggtaaaa ccttgttagg tgccgatgac aaagcaggta
     3421 ttgcagaaat catgaccgcg ctggcggtat tgcaacaaaa aaacattccg catggtgata
     3481 ttcgcgtcgc ctttaccccg gatgaagaag tgggcaaagg ggcgaagcat tttgatgttg
     3541 acgccttcga tgcccgctgg gcatacaccg ttgatggtgg tggcgtcggc gaactggagt
     3601 ttgaaaactt caacgccgcg tcggtcaata tcaaaattgt cggtaacaat gttcacccgg
     3661 gcacggcgaa aggagtgatg gtaaatgcgc tgtcgctggc ggcacgtatt catgcggaag
     3721 ttccggcgga tgaaagcccg gaaatgacag aaggctatga aggtttctat cacctggcga
     3781 gcatgaaagg caccgttgaa cgggccgata tgcactacat catccgtgat ttcgaccgta
     3841 aacagtttga agcgcgtaaa cgtaaaatga tggagatcgc caaaaaagtg ggcaaagggt
     3901 tacatcctga ttgctacatt gaactggtga ttgaagacag ttactacaat atgcgcgaga
     3961 aagtggttga gcatccgcat attctcgata tcgcccagca ggcgatgcgt gactgcgata
     4021 ttgaaccgga actgaaaccg atccgcggcg gtaccgacgg cgcgcagttg tcgtttatgg
     4081 gattaccgtg cccgaacctg ttcactggcg gttacaacta tcatggtaag catgagtttg
     4141 tgactctgga aggtatggaa aaagcggtgc aggtgatcgt ccgtattgcc gagttgacgg
     4201 cgcaacggaa gtaagcgaaa agggatgcgg catgtgatgc cgcatccggc ttaaatccaa
     4261 acttaccctt cgaagaacca gtatccgcta ttgaccagcg ccgcgagcat cgcgaggaat
     4321 gacggatctt ccagcgcatc gccaaaattc tccgcggtca gcgcaatgtt gctggcgagc
     4381 gcatccagtg ccggacggtg cggggaatcg atcttctcac cattggcata cacgtcgtcg
     4441 ccaatgcgca atacgcgcag accccccagg cgcaccagca catcaccttg tttcagcgca
     4501 tcgtagattt catccggctg ataaggcggc tctggcggcg cgatgtccag ttcatgacgt
     4561 gactgggata taaactcgcc aaaccattgt ttaaagtgtt ccggctggtt gatcaattcg
     4621 agcatcatct cacgcagttt atccatctct tgcggcagaa cgtccgcagg atgagcgcga
     4681 ggtggaacat caggatcgct gtagtagttg ccgcccagtt cacgttgcag cacataatcg
     4741 gcaaatccgc taatcagttc ccgcgtattt ggcgcgcgaa aacccacgga atagttcatc
     4801 gcattttcca gcgcgtagcc ttcatgcggg aatcctggcg gaatataaag aatatcgcca
     4861 ggctccagct cttcatcgat gatggcttcg aacggatcga cctgtaacag gtccgggtgc
     4921 gggcagtgct gtttcatttg cagcttttcg cccactcgcc agcgacgacg accggtaccc
     4981 tgaatgataa acacgtcgta ctgatcgaga tgcgggccga cgccgccgcc cggtacagaa
     5041 aaagagatca tcagatcatc aatacgccag tccggtagtt cacggaacgg tcgcattagc
     5101 gcggcggtcg gctcatgcca gtggttcact gcttgcacta acaatgacca gttagtttca
     5161 ccgagatgat cgtagctttc gaatggaccg tggctgacct gccatttgcc atcctgatga
     5221 ctgaccagtc gactgtcgac ttcgctttcc atcgccagac ccgccaactc gtctggagag
     5281 attgggtcaa taaaattatt aaagccgcgt tttaacacca ccgggcgttt ctgccagtga
     5341 cgttcaagaa aatcgggcca gttaagagtg agttggtatt ccatgttaag catccacagg
     5401 ctggtatctg caaccgatta taacggatgc ttaacgtaat gcgtgaagta tggacatatt
     5461 tattcatctt tcggcgcaga atgctggcga ccaaaaatca cttccatccg cgcaccgccc
     5521 agcatgctct ctccggcgac gattttaccc tcatattgct cggtgatttc gcgggctacc
     5581 gccagcccca caccttgccc agggcgtaaa gtatcaaccc gttgaccacg gtcgaaaatg
     5641 acctctcgct tgcttaatgg aataccgggg ccatcatcct cgaccacaat atagagatgc
     5701 tcgtcggttt gccttgcaga aatttcgaca aactcgaggc aatatttaca ggcgttatcc
     5761 agcacgttgc ccatcacttc gacaaaatca ttctgctcac caacaaagct gatttctggc
     5821 gagatatcga gagagatatt gacccctttg cgttgataca ctttgttcag cgccgaggtg
     5881 agattgtcca acagtggggc gaccggatgc agctcgcggc taagcaatgt cccgccgcgc
     5941 atactggcac gatgcaggta gtagccaatt tgctgtgaaa tgcggctgat ttgctccagc
     6001 attaccggct cagcatcact gacgctcatc ttctcactac gcagagaacg cagcgtactt
     6061 tgcagcaccg ccagtggcgt tttcagacta tgggtcaggt cggtgagcgt cgtacgatat
     6121 ttgtcgtaac gttcgcgttc actttttaac agtcggttca ggtttcgtac cagactggtc
     6181 agttctcgcg ttgtggctgg attgagcaat tcacggttat gttcttccag ttcgcggact
     6241 tcttttgcca gggcttcgat ggggcgtaaa ctccaccagg cggcgaccca cagcagcggg
     6301 atcactaaca gcagattggc tgagagcaca tagataaacc agctccagac catataggaa
     6361 ctttttagct ccaccggaat ggtatccacc accacaatgg ttaactttgg catccgcgat
     6421 gttgccgggt agacgtttac cgccaccgag tgggtcatct ccgcgtcgtc atcatcttcc
     6481 cgcacttcct gcaactgttg ctgtatcgaa tgatctccac tcagcaagag gctggtatcg
     6541 ttaacatccg cttcaatttc atgaaaacca ttcgatttca gccagtcagg ctggatcatc
     6601 ttcatcagcc agggcacgtc acgttgcgcc cataaaagct gcccgttctc atcataaatt
     6661 agtgtcatcg tggggctttg cttgtcgata ttttcgggta actcgacatg caacttattg
     6721 ttttcccact tcgcaagggt atagaacaga ttgctctcgc cacgtaacag ccgaaacgtg
     6781 gttttatcga aactgacgct ataaccgatc agcgcaacca ttccgtaggc aagcgaaagc
     6841 accaatacta ctgctgccgt tgctaacaga aaacgtaccc gcagcgagag cgggaaaaaa
     6901 agacgcagta attttttcat cagcgcaatt cgaacagata gccctggccg cgaacggtgg
     6961 taatcacttc ttgcggatat tgtgcctgta tttttttgcg cagacgtccc atcagtacat
     7021 ctatggtatg actttcccgc agctccgcat ccggatagag ttggagcatt aacgaatctt
     7081 tgctcaccac tttgccatta ttgcgtatca acgtttccat aatggtgtat tcgaacgcgg
     7141 tcagtttgat cacttcgtca ttaatagata attcacggcg ggagagatca acctgaaacg
     7201 ggggtagcga aatgacctgt gaagccagac cgctattacg ccgcattaat gcctgcattc
     7261 gcgccatcac ctcttcaata tgaaacggtt tagtcacgta atcatcagca ccggcactta
     7321 atacttcgac tttgtcctgc cagctttcac gggcggttaa taccagaatc ggcagtgaaa
     7381 catcattgct acgccagcgg cgaatcagtg acagaccgtc ctcgtcgggc aaaccgagat
     7441 cgacaatcgc aatatccggt aaatgttcat tgagataata atcggcttct ttggcatctt
     7501 ctgcgtcatc gacctgatga ccagcatcct gaatctgaac tttaagatgg tgacgtaaca
     7561 acgcattgtc ttcaacaacc agtacgcgca ttttttattt ctccctgtct taattattaa
     7621 aatagtgtaa cgcgattatg tggttatggg ggtaaacatt aaataaacca gcggggaggg
     7681 gaggtaaagt gaaaaaataa aaagtggata atcttaataa gcaggccgga cagcatcgcc
     7741 atccggcact gttttgaggc ttatttcagc tcatcaacca tggtgatggc gcgaccaatg
     7801 tagttagccg gcgtcatcgc tttcaggcgg gctttctctt cttctggcaa cgccagacca
     7861 tcgataaact gcttcatgcc ttcggcgtca acgcgcttac cgcgagtcag ctctttcagc
     7921 ttctcgtacg gtttttcgat gccatagcga cgcataactg tctggattgg ttcagccagc
     7981 acttcccagt tgtgatccag ttcatccagt agatggtcac ggttcacttc cagtttactc
     8041 acgcctttca gggtggattg atacgcaatc agcgcataac cgatacccac gccgaggtta
     8101 cgcagcacgg tggagtcggt caggtcacgc tgccagcggg aaactggcag tttgcttgcc
     8161 aggtgctgca ataccgcgtt ggaaagcccc aggtttcctt cggagttttc gaagtcgatc
     8221 gggttaactt tatgcggcat ggtggaagaa ccaatctcac cagcaatggt tttctgtttg
     8281 aagtggttaa gggcgatata accccagacg tcacggtcaa agtcgatcag aatggtgttg
     8341 aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt gcggttcgat ctgggtggtg
     8401 tacgggttcc actgaatacc cagcgaggtg acgaactctt cgctgaactg atgccagtca
     8461 acttccgggt aagcggcgat gtgggcgttg tagttaccga ccgcaccatt gattttgccg
     8521 aggatctcca cctgattaag ctggcggtac tggcgctcca tacggtaggc gacgtttgcc
     8581 atctctttac cgatggttga cggcgtggct ggctgaccgt gggtacggga cagcagcggg
     8641 atatcgcgat actgaacggc gagatcttta atgccatcaa tcagttgacg ccagtacggc
     8701 aggatcactt catcacgcgc ggttttcagc attaatgcgt gggagaggtt attgatatct
     8761 tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt gcagttccgg gatctccgcc
     8821 actttttctt tcaggaaata ctcaaccgct ttaacgtcgt ggttagtggt acgctcgatg
     8881 gttttgatgc gtgctgcatc ttcttcgctg aaactggcga caattgcatc aaggtaaccg
     8941 attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg cgtgcgcggc cagtttttgc
     9001 agccaacgta cttcaacttg tacacggaat ttcagcaaac cgtattcgct gaaaatcccg
     9061 cgcagcgcgc tgactttatc gcagtagcgt ccatcgacag gggaaacggc ggtcagtgag
     9121 gataattcca tagatcacaa ctccggggtt aaatgagcaa gaatttgttt tgcctgagtg
     9181 gtcaggcgat tacgagaaaa catcagttgc agacgaccgc cgccgacctg gtgccagagc
     9241 acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt tcgcctgcac ctgtgggctt
     9301 tgcagtacag caggggaacc agtgacctga atgcgcgggc caagcgggct gataacatca
     9361 acatagatgg cagccatcgc gctcatcagc gtttcggact gtaaatcgaa gtgttcgagc
     9421 tggcgttgca ggccgttgat ccggttgccc agagtgtcga gcgcaccttt cgctgaggag
     9481 agtttgcgct caagcaccat cagactgagt gtgtagcggg ttaattcggc gtttaagccc
     9541 tggcgactgc tggcattgag tacgccgagc aaggtttcca gcccgacgcg caggttggct
     9601 tcgctaccgc caaaaaccgc cagcgttgag ctggggttca tatcgataat actgttgagt
     9661 gagacgtgta gcgcatcgcc atcacaatgc ccctggtgag cgagttgttg caccaggcgt
     9721 gccgactgac aaataccggc aagggcgagg gtgatgtcat agtaattctt tgccacgttc
     9781 actgcttcct tgttttaagt aaagataata atcagaccgg cagcggcaga cgctgctcaa
     9841 taataccgcc accgaggcac acttcaccgt tatagaaaac ggcagactgg cccggcgtca
     9901 cggcggcaac cggctcatcg aaaatcactt caatgcgatc agcgtccagc gccttgacgg
     9961 tgcacgggat gtcggtctgg cgatagcggg tttttactgt gcaacgcata gtaccggtga
    10021 acggctcgcg atcgacccag tgcagctgct gggcaatcag accgacagac atcagacgcg
    10081 ggtgttcatg gccttgcgca acaatcagaa tgttgttttc gacgtcttta tcgaccacat
    10141 accacggttc ttcggtgccg tctttggtgc caccgatacc cagaccttta cgctgaccga
    10201 gggtgtgata catcagcccc tggtgctcac caatttcatc gccatcgacg gtaatgattt
    10261 tgcccggttg cgccgggaga taacggccca ggaactcgcg gaatttacgc tcgccgataa
    10321 agcagatacc ggtagaatct tttttcttcg cggtgaccag accgagatct tcagcaatct
    10381 tacgcacctg cggtttttcc agttcgccga ccgggaacag gctttgcgca atttgctcat
    10441 ggctgagcgt ataaaggaag tagctctggt ctttattgct gtccagacca cgcagcaggc
    10501 ggctcttgcc atcgacatct gcccgacgta cgtaatgacc ggtagcgata taatcggcac
    10561 ctaaatcttc ggcggcaaat tcgaggaaag ctttaaattt gatctctttg ttgcacagaa
    10621 tatccggatt tggcgtgcgg ccggctttat attcggcaag gaacagttcg aagacgttgt
    10681 cccagtactc ggcagcaaag ttaacggtgt gcagttcaat gccgagcttg tcgcagacag
    10741 cctgggcatc agccagatcc gctgccgctg tgcaatattc ctcaccgtcg tcttcttccc
    10801 agttcttcat aaacaggcct tcgacctgat atccctgttg ttgcaacagc caggcagaaa
    10861 cggaggaatc gacaccgccg gacatgccga cgattacttt ttttgcggtt tcagacattg
    10921 gatcactcac gacattgaac ttcaaggcgg cgtattctat cacgcagcct tttagatgac
    10981 accctttgta aaaggccagt taaaatcgcc aatcatctcc agcggatagc gttgcccgct
    11041 ttgataacaa cgaatacttt ccgccaccag cggcgaacga agatttgacg cctgtaaaat
    11101 ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg tcatgaggct gcgtcgggca
    11161 tatttgctca agctcaatgg caaagaggaa acgcaaaaac ggcgttttat ctggcgcaat
    11221 ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg atgccggttt cttcccacag
    11281 ctcacgggcg gcggcttcca ctaaggtttc atcggcttcc aggtgcccgg caggctggtt
    11341 ccataacgct ttaccattaa tcgtctcttc aacgactaaa aatttgcctt ctgcgtgcac
    11401 cacgcaggca acggtaacgt gcggtttaaa cattttgcat ccttaatctg tcgcttctcg
    11461 ccattcgcca ttggcaagat tatccaaaga gtaattcccc atcgcatagc gaatcagtcg
    11521 cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc tggcgattac gtccttcata
    11581 taaggtgatc ttcagccagc tggtgggaat acttttgcgc tcacgaattg gtggattccg
    11641 cggccataac cacgcgggtt cttcaaccag ctccgcgcct gcgggcaggg tagggccatc
    11701 atttaaggtt acaccattgc gcaaggcttc aagtgcgtct tgtgtgggaa taccttccac
    11761 ctgcacataa tagatttttc cggtgcgttt acccggctgg gttaaacgcg cctgcaacgc
    11821 gccgttattg gtcagcacca gtaacccttc gctatcgcgg tcaaggcgac ctgctgcata
    11881 aacgccttga accgggatga attcttttaa tgttttgcgt ccggcttcat cggtgaactg
    11941 cggaagaaca tcgtagggtt tattgaacag gatcacacgc gtgggctggt tgtcaggttt
    12001 tcgtctggta gaacgttgcg agctgaatcg cttaacctgg tgatttctaa aagaagtttt
    12061 ttgcatggta ttttcagaga ttatgaattg ccgcattata gcctaataac gcgcatcttt
    12121 catgacggca aacaataggg tagtattgac aagccaatta caaatcatta acaaaaaatt
    12181 gctctaaagc atccgtatcg caggacgcaa acgcatatgc aacgtggtgg tagacgagca
    12241 aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag tagttgttcc ggcacaaggc
    12301 aagaagatca ccctgcaaaa cggcaaactc aacgttcctg aaaatccgat tatcccttac
    12361 attgaaggtg atggaatcgg tgtagatgta accccagcca tgctgaaagt ggtcgacgct
    12421 gcagtcgaga aagcctataa aggcgagcgt aaaatctcct ggatggaaat ttacaccggt
    12481 gaaaaatcca cacaggttta tggtcaggac gtctggttgc ctgctgaaac ccttgatctg
    12541 attcgtgaat atcgcgttgc cattaaaggc ccgctgacca ctccggttgg tggcggtatt
    12601 cgctctctga atgttgccct gcgccaggaa ctggatctct acatctgcct gcgtccggta
    12661 cgttactatc agggcactcc aagcccggtt aaacaccctg aactgaccga tatggttatc
    12721 ttccgtgaaa actcggaaga catttatgcg ggtatcgaat ggaaagcaga ctctgccgac
    12781 gctgagaaag tgattaaatt cctgcgtgaa gagatgggcg tgaagaaaat tcgcttcccg
    12841 gaacattgcg gtatcggtat taagccgtgt tcggaagaag gcaccaaacg tctggttcgt
    12901 gcagcgatcg aatacgcaat tgctaacgat cgtgactctg tgaccctggt tcacaaaggc
    12961 aacatcatga agttcaccga aggcgcgttt aaagactggg gctaccagct ggcgcgtgaa
    13021 gaatttggcg gtgaactgat cgacggcggc ccgtggctga aagttaaaaa cccgaatacc
    13081 ggcaaagaga tcgtcattaa agacgtgatt gctgatgcat tcctgcaaca gatcctgctg
    13141 cgtccggctg aatatgatgt tatcgcctgt atgaacctga acggtgacta catttctgac
    13201 gccctggcag cgcaggttgg tggtatcggt atcgcccctg gcgcaaacat cggtgacgaa
    13261 tgcgccctgt ttgaagccac ccacggtact gcgccgaaat atgccggaca ggacaaagta
    13321 aacccaggct ctattattct ctccgctgag atgatgctgc gccacatggg ttggaccgaa
    13381 gcggctgatt taattgttaa aggtatggaa ggcgcaatca atgcgaagac cgtaacctat
    13441 gacttcgaac gtctgatgga aggcgctaaa ctgttgaaat gttcagagtt tggtgacgcg
    13501 atcatcaaga atatgtaatc actacatgtg ttaaatatta taacgggcgt ataacacgcc
    13561 cgttgttttt tgttagcctg ctaacggtta tcaaaatttt atcaaaataa gttatcaaaa
    13621 ccccttggtt gttggggggc tgccagatgg cagttctcct gctaggttcc ctcggggcat
    13681 tcctcagctt tttattattg ctggtggatg gccgttaata tttaagatct gttatgcgcc
    13741 agacgctgtg cttactagaa tattgttgtg tcaaatggtt tggtatactc tgattgtggc
    13801 taatatgtaa atcattttaa ctaataatta aaatttctga gggtactcga gttttcttat
    13861 atatttaact ttctcgcaat atacatttac gtttctgttg ttgtttttga tataattcaa
    13921 tgagtctcaa caaaacaaga ttttcggaaa ttatatggat atcattattg taccccgaga
    13981 ccaacgtatt cctgcctcag gcattagtac ggcttacttg catgtggatc attggaatga
    14041 cttttcattc attacaatgt tttacatgta tctgtttgat cagaaaggtg aacgccatga
    14101 aattgggaat gtcaagattg gttttcaagg acaaacaact caacaatcaa cttactccac
    14161 attaggggac aggtttaaaa ttctcccaga aggatatttt tccgttggtc aggatgtcga
    14221 ctattatcag aggatttcaa atttaccaga atccgtcaaa gtttcgttac ttgaggcgct
    14281 caaagatatt gcttatacgc cagaacttat agatttcgtt aagaatgaag cggtctttaa
    14341 aacgtctttg cttcggtatg ttagcctgtc tgttattaaa ggacagtttg cgcgagtgct
    14401 tgagggaaag agtccactga ctaattttga gtttaagttc attcgccctg cacaagacaa
    14461 aatttccgat attgaattat catttaaggt aaaagtagga gaaaaaccaa gtaccaatat
    14521 tcatgcgatc ataggtcgta atggtgtggg gaaaacgacg attctaaatg gaatgattga
    14581 agctgttacc agtaaaggta cgtcgaacgc aaaattttat gatctggagg ggtggagaga
    14641 agacccgatt gataatgatt actttagtag tttagtctct gtgtcattta gcgcattcga
    14701 tccattcgag ccaccaagtg aataacctga tccatcaaag ggaacttgct acttctatat
    14761 aggtttgaaa aaaaaaggag agtcgctgaa agggctaaat gatattcatg aagactttct
    14821 agaagctttg acgctatgct ttagccagca tcctaagcgt gaaagatggc tgaaggccat
    14881 tgatactttg gagtctgatg aaaattttgc aagcatggag ctgaaaaatc tagcggaata
    14941 caatgatgag atgctcgtta gtaatgcacg gaaatgtata aaaaggatga gttccggtca
    15001 tgcggttgtg ttattaacac tcacacgttt agtcgcaacg gtagaagaaa aaacgttagt
    15061 gctaattgat gaaccagaga gccatcttca tcctccgttg ctgtctgctt ttattcgtgc
    15121 gctgtctgat ttgctacttg acagaaatgg tctttctatt attgctactc actcacctgt
    15181 ggtacttcaa gaggtcccta aacgatgtgt ttggaaaatt aatcgttcta gattatctac
    15241 cgaatctcgc aggccagaca ttgaaacatt tggtgagaat gtaggaatct taacaagaga
    15301 ggtgtttggt cttgaagttg tcaaatctgg ttttcacgat ttattagtcc aatcagttaa
    15361 gtcaggtgga acatatgagg agatagttga tgagtatcgt aaccagcttg gtgttgaagc
    15421 tcgagctcta ttaaaagcgc ttgtcacaca cagagatcgg agtgttaagt aatgttgaaa
    15481 cttaaacaac ctgcttacac atatgccgat atgctgacta gttgccgcga aggcataacc
    15541 ggtaatccgg gcttgttgct gaaagtaaat gaagcaataa ctgatttgca aacactggca
    15601 gcttcatata gcgcatcggc atcaactggt gagctttata caatacaacc attgaaaaac
    15661 aaagataagg aagaccctat tgttgtcggt cggttaaaga aatttgactt tgttaagctt
    15721 tatggcaatt atgttgttgg gaagaaaaag cctgctcgag ctatttatga ttccctgatt
    15781 actgctgcta atgaaaaatg tccatattgt ggtggaattg ggcgccctcg aaatttagat
    15841 cattatttgc ctaaagctta ctatcctcag ttttctatat tgccagttaa tctggtgcct
    15901 gcttgtcgag actgtaatat ggatggcaaa ggggaagttt ttgcaagaaa tgaagcagaa
    15961 cagatcatac atccctattt agacgatagc cgttattatg atgaacagtg gatatcggct
    16021 agatacttgg ctggcaaaaa tgatgagcca ggagttattg agtattttgt ttctccaccg
    16081 gctgattggg atgaagcaca caaacaaaga gtggaaaaac attttaacga ttttgatttg
    16141 tctttaagat attcgaaaga ggctagtgcg agattagttg tacttttagc tctatataac
    16201 tcagtgctac cactctccag agataaaaat gctgctaaac accttatatt tcaaacaata
    16261 attaataaat cgccttttgt gaatcattgg gaacgggtta tgtgtttggc tcttatgtca
    16321 gacttgtagt aatgcttgaa gtttttattt ctgttctaat ttcgtagttc cattttatta
    16381 aatagatacc aagctctatg cataagagct tggtatctat tgaaattaag ctcaataact
    16441 gatctgctct ccattgatta acacgtcaca atattagtaa tgtaactatt aggtttcgag
    16501 attaactgcg aacttctgct ttttgctcaa agtggactgt ttggttcttt ttcaagtgta
    16561 gttcgaaatt caaggtggcg ttaaggcact taatgcttta caacgctact ttactccatc
    16621 ctttacctcg atcatcatga taacgatcgg tttgctgttg cgttttatga ccaagtagtt
    16681 tttgtgtatt taaaccctgt tctttataca aacgttctga taaagacctt tgttcatgaa
    16741 atgttgcggg agaaccttca ccccagttaa ttcctgctaa atccctcgct ttactgaaat
    16801 tcatcgtcaa tgtattggct ttaacctgcg cgccgcgttc agcctgtgag gtagtacgaa
    16861 aaaaatgtac aaggtatggg ctgacagcat agtcacggca gcgggctact acatcgcgca
    16921 aactccattt aatcgcattg agacgcaggg aaagcgggat ggcgattttg ctcccggttt
    16981 tttcctggat gacatggaga tgatcatccc aaatatcgct aaatttcata cgcgagatat
    17041 cacctaacct ctggccggta accagtgcta acagcatggc attccccata taacgatgac
    17101 tggcatcagc gatatcaaag attttttgcc actcttcaag acttaaccgt tgtcgggtaa
    17161 tttttcttct gggctgtttg gtggctaatg ccgggttata gccaggggga cttctccgta
    17221 gtgttgcgcc tctttgaaaa catcaatcag tacggaacga actacttgcg ccattctcgg
    17281 ctgtccggcg gcgatatact cgtcaagcaa ctgggctata tccctgacat caacggctga
    17341 aattaacttc agtcctgctc gttccctgag caaggatact ggtttggctt tctgtttata
    17401 ggtgttgagc tttatatcgc cacttttcag tctgtcttcc tggattgctt gatagcgatc
    17461 taaccaggtt gacgttgtga ttgcttttcc tttgctggtt gcgatcctgt cactgatagc
    17521 tagaatctgc cgggttcttt gctcagctag gcgtgtattg gcctcagtgg caatagcaat
    17581 ggcttcagct tcgtttgtgc ccaaagaatg aaacttcccg gtcactggat gcttatatcg
    17641 ccaataaact ttatttactt ttctgctata gagcggatac aagttcggga ctgaaacatt
    17701 attcttacgt ggtctggctg ccattactta aaatcctttg tagaataata gaatcacttt
    17761 tcttaatgac aggagtaact aattctccaa ctaactctgc gtcctcacgt actcgccata
    17821 gtcgaccttg tttcattgcc ggtgtgtagt ggcacactga atttggccac ctgaacagag
    17881 gtgatatgct cacctcagaa caacacaggt gctccaatga aaaaaagaaa tttcagcgca
    17941 gagtttaaac gcgaatccgc tcaactggtt gttgaccaga actacacggt ggcagatgcc
    18001 gccaaagcta tggatatcgg cctttccaca atgacaagat gggtcaaaca actgcgtgat
    18061 gagcgtcagg gcaaaacacc aaaagcctct ccgataacac cagaacaaat cgaaatacgt
    18121 gagctgagga aaaagctaca acgcattgaa atggagaatg aaatattaaa aaaggctacc
    18181 gcgctcttga tgtcagactc cctgaacagt tctcgataat cgggaaactc agagcgcatt
    18241 atcctgtggt cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga
    18301 aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagtcag gtacttgagt
    18361 tgcataacat caggttctgc cggggcaaga agcatcgcca caatggcaac cctgagaggc
    18421 ttcagaatgg ggcgctggct tgccggcagg ctcatgaaag aactgggact ggtcagttgc
    18481 cagcagcctg cgcaccgtta taaacgaggt ggtcgtgaac atgtcactat cccgaatcac
    18541 cttgggcggc agttcgcagt gacagagcca aatcaggtat ggtgcggcga cgtgacgtac
    18601 atctggacgg ggaaacgttg ggcatacctt gccgttgttc tcgacctgtt tgcaaggaaa
    18661 ccggtaggtt gggcaatgtc gttctctccg gacagcagac tgaccatcaa agcgctgaaa
    18721 atggcctagg aaatccgcag taaaccagcc ggggtaatgt tccacagcga tagtaataat
    18781 gccggtatca gtttttatca tcactctgtt tgctgtttaa ccagactggt gtgattactg
    18841 atgcagtgaa gaccttcccg catcctgact cacacagcga tcgacccttt gtgtcctgcc
    18901 ctggacctgt cggttgccgg aagcgccttc atgcgaggcg tctcctcacc gatgcgcgtg
    18961 actcaagaag ggcctgacgg tttgtctcgt tactgtcctg tccgggttat ctgtctggag
    19021 attcaactct gtttcctcac aggagctctg ttatggcagg taaagttacg gaaaccgctg
    19081 ttgtgggtgg cgtggataca cataaagatc tgcacgttgc cgctgtcgta gatcagaaca
    19141 ataaagttct ggggacccag tttttctcca caatacggca aggttaccgg cagatgctgg
    19201 catggatgac ttcgtttggg gcattaaagc gaattggtgt tgagtgtact ggcacctatg
    19261 gatcaggtct gcttcgctat ttacagaatg ccgggttaga cgttcttgag gtgactgcgc
    19321 cagatcggat ggagcgacgc aaacggggta aaagtgacac gattgatgct gaatgtgccg
    19381 ctcacgccgc attctcccga ataagaaccg tcacacccaa aacgcgcaat ggcatgattg
    19441 agtctctgcg ggtattaaaa acttgccgaa aaacagcaat atcagcccgc agagtcgctc
    19501 tccagattat ccattccaat attatctctg ccccggatga attacgtgaa cagctcagaa
    19561 atatgacgcg catgcagctc atcaggactc tgggatcctg gcggcctgat gccagtgaat
    19621 accgcaatgt taccaacgtt tatcgtattt cattaaagtc ccttgcccga cgctatctcg
    19681 agttacatga cgaaatcgct gatttggatg tcatgattgc ggcaattgtc gatgagctgg
    19741 cgcctgaact gattaaacgt aatgctattg gatacgaaag cgcttcgcag ttgctgatca
    19801 cggcaggaga caatccccaa cgattaagat cagaatcagg ttttgcggca ctgtgtggtg
    19861 tcagccctgt tcccgtatct tcaggaaaaa cgaatcgtta tcgacttaac cggggtggag
    19921 atcgtgctgc aaatagtgca cttcacatca ttgccatcgg acgtttgcga actgacgata
    19981 aaacgaagga atatgtcgcc agacgagtag cggaagggca tacaaaaatg gaagcaatac
    20041 gctgcctgaa gcgctatatc tcacgcgaag tttatacatt actgcgtaat caaaacaggc
    20101 agctcaacag catcccgata acggcttgac tcttagaagg gcgtccaggg cagccactat
    20161 acaagcaggc agttccggca gttactgtgg cgttaccaga tcaaacagag tctgagtcga
    20221 cgaggaaatt gctgggataa cagcccgatg gagcgcttct tcaggagtct gaaaaacgag
    20281 tggataccgg tgacgggtta catgaacttc agcgatgctg cccatgaaat aacggactat
    20341 atcgttgggt attacaacgc gctcaggccg cacgaatata acggtgggtt gccaccaaat
    20401 gaatcggaaa accgatactg gaaaaactct aaagcggtgg ccagtttttg ttgaccacta
    20461 caaaacactg ctgagatatt caattttcca gtgccggatg tggcacaaaa ggagcggcgc
    20521 gtggcagatc tcgacgatgg ttatacgcgc attgcaaatg agttgctgga agctgtgatg
    20581 ctggccggat taacacagca ccagtttctg gtcttcctgg ctgtcatgcg caaaacatat
    20641 ggctttaata aaagactgga ttgggtgagc aacgagcaac tttccgaatt gaccgggata
    20701 ttgccgcaca agtgttctgc tgcaaaaagc gttctggtaa agcgtgggat ttttattcag
    20761 agcgggcgga atatcggcat taataatgtg gtcagtgaat ggtcaacatt acccgaatca
    20821 ggtaagaaaa ataaagttta cctgaaagag gtaaatttac ctgaatcagg taagaaaagt
    20881 ttacccaaat caggtaaaca caaaagacaa actaacaaaa gacaatataa aacctttttc
    20941 gtccgagaat tctgacgaat cctctgacca gccagaaaac gatcttcctg tggtgaaacc
    21001 ggatgctgca attcagagcg gcagcaagtg ggggacagca gaagacctga tcgccgcaga
    21061 gtggatgttt gacatggtga agaccatcgc gccatcagcc agaaaaccga atttgcaggg
    21121 tgggctaacg atatccgcct gatgcgtgaa cgtgacggac gtaaccaccg cgacatgtgt
    21181 gtactgttcc gctgggcatg ccaggacaac ttttggtctg gtaacgtgct gagcccggcc
    21241 aaacttcgcg acaagtggac ccaactcgaa atcaaccgta acaaacaaca ggctggcgtg
    21301 acagccggaa aaccaaaact cgacctgaca aacactgact ggatttacgg ggtggattta
    21361 tgaaaaacat cgccgcacag atggttaact ttgaccgtga gcagatgcgt cggatcgcca
    21421 acaatatgcc ggaacagtac gacgaaaagc cgcaggtaca acaggtagcg cagatcatca
    21481 atggtgtgtt cagccagtta ctggcaactt tcccggcgag cctggctaac cgggaccaga
    21541 acgaactgaa cgaaattcgc cgccagtggg tgctggcttt ccgggaaaac gggatcacca
    21601 caatggaaca ggttaacgca ggaatgcgcg tagcccgtcg gcagaatcga ccattcctgc
    21661 catcacccgg gcagtttgtt gcatggtgcc gtgaagaagc atccgttatt gccggactgc
    21721 caaacgccag cgagctggtt gatatggttt acgagtattg ccggaagcgt ggcctgtatc
    21781 cggatgcaga gtcttatccg tggaaatcaa acgcgcacta ctggctggtt actagcctgt
    21841 accagaatat gcgggccaat gcgcttactg atacggaatt acggcgcaag gctgccgatg
    21901 aactgacttg tatgaccgcg cgaattaacc gtggtgaggc gatacctgaa ccagtaaaac
    21961 aacttcctgt catgggcggt agacctctaa atcgtgttca ggcgctggcg aagatcgcag
    22021 aaatcaaagc taagttcggg ctgaaaggag tgatgaaata gagctaaagt attagcttaa
    22081 aaataaagta tacctagcga atataatcta ggactagttg ttatgcccga aatgattgat
    22141 tgcgtgagta acaaagtgtc tcttccattg ttgtgaaatc atttagccat aaagtttatg
    22201 cagagatttt attttatttt tgagttgatt tttttgtaac gatctgtata tttcgcgcct
    22261 cttgtcatac cagttatttt tttcattata ttcataacgt tgattgaact atgaccactg
    22321 tgaataaaaa gttaaaaaaa actgcatctg gcgcgattac atggtcggta attgttaccc
    22381 agatattatc tccagtttct ctttctttga tcccagcaaa cagttttgca tcatctggta
    22441 ataaagatgt tacgcaaact tatgccagcg atgagcatgc aaataaagtg gcctcatttg
    22501 cagcaagtgc aggtcagagc ctggcgaata ataatgcaag tagttttgct gtaaatactt
    22561 tatcaactca ggcgacaaaa gaggtcgtcg actggttgca acaatatggc aatgcgcgca
    22621 tcaagcttaa tgtcgatgat tctttttcct tgaaggattc atcattcgac tttttatatc
    22681 catggctgga tactcaggat tatgtgttat tcagtcagac atcactacat cgtactgatg
    22741 accgaaatca gaccaatatt ggtttgggga ttcgtcattt cactcctgat aacgcaatgt
    22801 tgggtgcgaa tgttttttat gattacgatt taagtcgcag tcactctcgt gcaggttttg
    22861 gggttgagta ctggagggat tatttcaggc ttggtgtaaa tacctatttt ggtctatctg
    22921 actggaagaa cagtcgggat attgatgatt atctggaaag acctgcaaat ggctgggatt
    22981 tttctgctga aggatggcta cctgcttatc cgcaattagg ggcatctatt cagtttgaaa
    23041 aatattatgg taaaaatgtc gggttatttg gaagcgataa ccttcaggaa aacccttacg
    23101 cagttactgg gggaatttct tatacaccag ttcctctgat taagttttct gcacagcata
    23161 agcaaggaca gagcaacgtt cacgatacaa ccttcggtgt tgagtttaat tatcgcccgg
    23221 gcgtttccct ggctgaacag cttagcagcg ataatgttgc agttatgcga gaagtccaga
    23281 atcggcgtta tgattttgtt gaacgaaata acaacattgt tctggagtac aagaagaagc
    23341 atgcactgaa aatcagtttg ccagagtctg ttcaggggga gggggaatca atcatccctg
    23401 taacactgac aatcaacaat gctagtggtg gtatcaagtc tgtacagtgg aatgatagtg
    23461 cattcactgc ggctggcggc aagatcagtg gaaatggcac atcatggcag atcactttac
    23521 cggcttataa aagcgaaggt gtaaattcgt ggaatgttgg agccacagtc caggataaca
    23581 gaggcaatgt ttccaactat gccgttatga atatcagcgt taataatagt ggtgtctcga
    23641 cagcggattc atcttttacg ctggatgggg attcgtctcc gacgatctct gctgatggtc
    23701 aatccactta tccaatagta ttaagcctga aagacagtaa cggtaaggca ttaaccggac
    23761 tggctgatga cattgaaatg tctgtggaat ttactgcgga tagcaatagt gctcgacagc
    23821 gtgaaacggt aactgctctg tcattaggcg cggtagagga aatctctgct ggtgtatatc
    23881 gctctgttct ggctgctggt tcgcaggctg ggacggtacg tgtaactgca aaagttcagg
    23941 gaaaaacctt tactctgaat attaagcagg cagccgttat tgatagtgat gtatcgacag
    24001 cggattcatc ttttacgctg gatggggatt cgtctccgac gatctctgct gatggtcaat
    24061 ccacttatcc aatagtatta agcctgaaag acagtaacgg taaggcatta accggactgg
    24121 ctgataacat tgaaatgtct gtggaattta ctgcggatag caatagtgct cgacagcgtg
    24181 aaacggtaac tgctccgtca ttaggcgcgg tagaggaaat ctctgctggt gtatatcgct
    24241 ctgttctggc tgctggttcg caggctggga cggtacgtgt aactgcaaaa gttcagggaa
    24301 aaacatttac tctcagtatt aagcaaacag cagcaacaga acctgattct gaggtcagtg
    24361 ctgttttaac cgctgcacca gcagaacagg tagttggtta caatatcaat ctacaactgg
    24421 cagtgaagga ttcacaagga aatgcaatca ctggtgataa cactctgagc ttttatgctc
    24481 ttaaccaggc agaaggagtc gagtttggga ctgtaacgga aaaagacggt gtctatagcg
    24541 cgacagtaac ttcaaaacga gctggtaaga ttactatcgg tgttaaatct gatagtcatg
    24601 atttctcagg tgttaagaaa gaaatcacct ttattgagga ccggcagcag gttacattct
    24661 ctcagtttga agccagccaa aataatgcat tggctgatgg aaaacagaga aatatggtaa
    24721 ctgtaagcct tgcggaccgt ttcggcaatg ttgttcctgg ttatgctgtc acattgtctt
    24781 taccagcagg gataactcaa gttggtggtg aacatgctgt ttcaactgac gaaaatggta
    24841 atgctatttt tgcgttaata agctccactc cggggtctta cgtgattaca gctcatgctg
    24901 gttctcagat gtctactgag ttaacagtga cctttgcatc aaatatgacc ggagcttcac
    24961 tgtcactgac gcctgaaagc agtagtttaa tttccaatat tcctgctaat ggtaaggatg
    25021 cagcggtatt agatgtgcag ctgacaaata cgaatgcgtc ggtcaatggg caaaagattc
    25081 agctcatcac ctcatctgaa ggattatctg taccaacaaa tatcgtgact gattcaacag
    25141 ggcatgtttc tgttccgctt acaacggtga aagctggtga gtacacggtg actgcacggg
    25201 tgactgacgg ttcccatagc gtggaatctg gcagcgttaa gttaactttt gtgcccgatg
    25261 ttgcttctgc agaacttact atgagtgtat caaaacagga gattgtggcc gatggcagtg
    25321 aaaacgcaac agtaaacatt cagttggttg atgcgaataa caatgcgttt acaggtgagg
    25381 tggatttaac gattacacca tcgacgggag catcattgac cagtagtaat ctgcaactgg
    25441 atgctcatgg acaggcaaca acgcagttca ctgcatcgaa atcaggccag tacacaattc
    25501 aggccgagta tgttctggat gggaaacgca taaccgcaag ccagaatatt gatgccgtga
    25561 cggatgtgaa gggggctgtg ttggagatca catcatctgc atcttcggct gtagtgagcg
    25621 ataccaacaa tctggtattt acgttactgt tgaaaagtaa gtcaggcgaa gcattaagtg
    25681 gtcgagcatt gaatgttaag acatctgggc cgtctaaata tggtgcgttg gtggttgata
    25741 aaaccacagt aacgactgat gagaacggtc aggctacggt aagcgtacat ggtcgaacgg
    25801 ttggttcgta taaattaacg gcgaccttaa acgaacttgg cagtgatgtt agcgcggtga
    25861 aatcattctc cctgtacgct gatgacgcaa atggagtatt gactctaaca aaagatcctg
    25921 gttatgagac aaacgatggc tctccagtag gtatttatgc tcgatttgtc gatcattttg
    25981 gaaacccatt atctggtacg gttgagtttt ctgctggtag tgaagatagt ccagactctc
    26041 aacgggtcaa aatggagcct gctacagtaa cgttacactg gacaggtaat gcagcttcgg
    26101 aattcagcac ctatgaaagt ggttatcact ggattaaggc caaaataacg aatagcaaaa
    26161 atacatatga gaaaacaata aggacttatg ttgtaaaact acctgaaaag gatagttaac
    26221 aaaattgaat ggggagggct tagccctccc tctttttaca ggttagcgtt tgggttttgt
    26281 tgttggggag ctgtttttat aggcgctccc aggtttttag taaaccactc aacgactctg
    26341 tttataataa ttggctgata ccatgtctta tcgccatgct cggccccttc taccagcacg
    26401 tactcagcgt tatcgccgtt cttcttcaac atcctgaata gtttggcgct ttgctcaggt
    26461 gaaaccagag tgtctttgct accatgcata ataagaaatg ggggttttac tcctttcata
    26521 tgtccgattg gactggcgtt tagcgctttt tcttttgacg ctgtgatggg ggctcccgca
    26581 aaatttctga atgcagggcc attgatcatt aaggcttcgg ttacggcagg agagcgatga
    26641 acctcctgca ctgattcagg gaacccctcg ccaatattca acaagtcaga aatcccataa
    26701 agtgtggcaa ctgcctgaac atctgaggat tgctgaagaa agtcaccttt atcaaaggtt
    26761 ttgtcaccat ttgtagttcc catcatctga gcaagccatc caccggcaga gtcacccaga
    26821 actccgattc tttgaggatc aatcccataa ttgctggcat gctctctcag gtaacgtatt
    26881 gcagcttttc catcctcaac tggtgctgga aatgtatcag gaattgttct gtattctaca
    26941 gcggccacaa caaaaccagc ttctgccaga gccattctca tttcaataaa tttgtcatgt
    27001 tcagaagaca tgaatccgcc gccgggataa taaataatgg ctggttttaa atcatttgtt
    27061 cgcgggacaa gaactgacat gtgaagctgt ctgacagaac gagttccttt tatctgggaa
    27121 taaacaacat caccaatgag atcgacttgg tttctggtcg gtgaaacact aatgatatct
    27181 gcacccgggg tgtaaccagg aagattagtc tggacaggtg ttgcacaact cccgaccgac
    27241 atggccattg acataccata caatagtgtt gcagatgata atatcttgtt aattttcatg
    27301 ttttacatgc cttgacttat cggatgaaat tctcttactg aatttaacgg cagtttaata
    27361 gccgtacaac tcgtggtagc cagggaaata tcccagtcgt ggttaaggtt gtacttgacg
    27421 gctattattt caacaatgct taaagtgaga gcttaaggtt atgcgtatgt tttacagtcc
    27481 gagtagattc tgaccaatag cttctttgta taattctgtc agaaataaat cttggcttgc
    27541 atgaagtttg agaccttatc ttgtttgatt atgaataatc aattcgccat aattgtatca
    27601 ccggagcctg aacaactccg gtgacttctg cgctaaacgg ggacgtttat gcgcacacac
    27661 aatccaaact ctcttctccc ttcacagatg cagaaatgca cctgcgtttt tttgcatcca
    27721 gcgtctgacc tctgtggagg tgaagcgtga acctaccaca agatggcatc aaactgcatc
    27781 gcggtaactt catcgctatc ggccagcaga tccagcctta tctggaggac ggaaaatgct
    27841 ttcgcatggt gcttaaaccg tggcgtgaga aacgcagtct ttcccagaat gcactcagcc
    27901 atatgtggta cagcgaaatc agtgaatacc tcatcagcag ggggaaatcg ttcgctaccg
    27961 cagcatgggt aaaagatgct ctcaaacaca catacctcgg ttatgaaacc aaggacctgg
    28021 ttgatgtcgt aaccggcgaa atcactacta tccagtcgtt acgccatacc tccgatcttg
    28081 ataccggaga gatgtatgtc ttcctgtgta aggttgaagc ctgggcgatg aatattggct
    28141 gccacctgac tattccgcag agctgcgagt tccagctgct gcgcgacaag caggaggcgt
    28201 aatggctaca ccgcttattc gtgtcatgaa cggacacatc tacaaagtac caaatcgtcg
    28261 taagtgtaaa cctgagctga agccatccga aataccaaca ctgctcgggt ataccgccag
    28321 cctggttgat aaaaaatggt tgcgactggc agcaaggagg aatcatggct gatttaagaa
    28381 aagcagcgcg tggtcgggaa tgccaggtaa gaatccctgg cgtatgtaat ggcaatcctg
    28441 aaacgtcagt actggcacat atccggctgg ctggattgtg cggtaccggt atcaaaccgc
    28501 cagacctgat tgccaccatt gcatgttctg cctgccacga cgaaatcgac cgtcgcacgc
    28561 attttgttga cgctggatat gcaaaagaat gcgcgctgga aggtatggcg agaacacagg
    28621 ttatctggtt gaaagagggg gttattgagg cgtgaatacc tacagcatca cattaccctg
    28681 gcctccgagc aataatcgct attaccgcca taatcgtggg cgcacgcacg tcagcgcaga
    28741 ggggcaggca tactgcgata acgtcgcccg aatcattaaa aacgcaatgc tggatatcgg
    28801 cttggctatg ccagtgaaaa tcagcattga gtgccacatg ccggatcgcc gtcgccgtga
    28861 ccttgataat ctgcaaaagg ccgcttttga cgcactcacc aaagcaggtt tctggctgga
    28921 tgatgctcag gtcgttgatt accgcgttgt gaagatgccc gttaccaaag gtgggaagct
    28981 ggagctgact atcactgaac tgggagatga atgatgtttg agtcttatat ggcagagcgt
    29041 cttcgccgcc gctgggtgcg cctgcgctta tatcgttttc ctggttctgt tttgaccgat
    29101 taccgaatac tgaagaatta cgccaaaacc ctgacaggag caggagtatg aagtcagaga
    29161 taacaatcaa ctaatactgt tttgttgatt tttgcttgta attggcgttc tggtctgatt
    29221 tttgtggagt aagttgatgc gtgatattca gatggttctt gagcgttggg gagcgtgggc
    29281 ggctaataat catgaagatg tgacctggtc gtccgttgcc gccggtttta agggattaat
    29341 tccttcaaaa gtaaaatctc gcccgcaatg ttgtgacgat gacgcgatga tcatttgcgg
    29401 gtgcatggcc cgtctgaaaa agaacaacag cgatttacac gatttattag tagattatta
    29461 tgtatgtggt atgacattca tgtcactggc aagtaagcat tgctgctcgg atggttatat
    29521 cgggaaaagg ttacagaagg ctgagggcat aattgaaggg atgttaatgg cattagatat
    29581 ccggttagat atggatatcg ttgctaataa ttctaattga tatgcaattg tttactaaaa
    29641 gttattaaaa atggggcgtg gaaacgc
//
LOCUS       DAZFRE010000062        29548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFRE010000062 DAZFRE010000000
VERSION     DAZFRE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.062"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            44..562
                     /locus_tag="ACHKUI_003080"
     CDS             44..562
                     /locus_tag="ACHKUI_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6599234.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            578..1117
                     /locus_tag="ACHKUI_003081"
     CDS             578..1117
                     /locus_tag="ACHKUI_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6599235.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1309..1533)
                     /locus_tag="ACHKUI_003082"
     CDS             complement(1309..1533)
                     /locus_tag="ACHKUI_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599236.1"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     gene            complement(1530..2267)
                     /locus_tag="ACHKUI_003083"
     CDS             complement(1530..2267)
                     /locus_tag="ACHKUI_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6599237.1"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     RLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     gene            2415..2747
                     /locus_tag="ACHKUI_003084"
     CDS             2415..2747
                     /locus_tag="ACHKUI_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6599238.1"
                     /translation="METINAVAALAAIAQESRLAVFRLLVQAGPAGMAAGKISEAAGI
                     PPSSLSFHLKELAHAGMVTSRQEGRFVIYEANFSTATNLVAFLTENCCGGQVCNLSCT
                     TEAGKVLA"
     gene            2744..3511
                     /gene="arsH"
                     /locus_tag="ACHKUI_003085"
     CDS             2744..3511
                     /gene="arsH"
                     /locus_tag="ACHKUI_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HFO6599239.1"
                     /translation="MRLRHLSDPDSLPALDKSFAIERPALGLAPDAPPVRILLLYGSL
                     RARSFSRLAVEEAARLLQFFGAETRIFDPSDLPLPDQVQSDDHPAVKELRALSEWSEG
                     QVWCSPERHGQITSVMKAQIDHLPLEMAGIRPTQGRTLAVMQVSGGSQSFNAVNTLRL
                     LGRWMRMFTIPNQSSIAKAFQEFDAAGRMKPSPYYDRIADVMEELVRFTALVRPHREA
                     LTDRYSERKAAGHVIDEATDLSSIAIAPQPLPESETS"
     gene            3508..4014
                     /locus_tag="ACHKUI_003086"
     CDS             3508..4014
                     /locus_tag="ACHKUI_003086"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HFO6599240.1"
                     /translation="MTERIYNVLILCTGNSARSIMAEALINTMGQGRFRAYSAGSHPT
                     GKVNPFAVEKVESVNYPTENLRSKSWDEYATPDAPKMDFIITVCDNAAGEMCPVWPGQ
                     PISAHWGFEDPAAVEGTDAEKRRAFEQTFRHMMNRVRLFVNLPLKMLDQTAIKRELAN
                     IGKTEQEA"
     gene            4011..5078
                     /gene="arsB"
                     /locus_tag="ACHKUI_003087"
     CDS             4011..5078
                     /gene="arsB"
                     /locus_tag="ACHKUI_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HFO6599241.1"
                     /translation="MSAGAQAIAAKPRQAPMSGFERYLTLWVALCIVAGVALGQGLPD
                     VFQAIGRMEVAQVNLPVGLLIWVMIVPMLVKIDFGALHQVKEHWRGIGVTLFVNWAVK
                     PFSMALLGWLFIRQVFAPFLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDPLFT
                     LSQVALNDAIMIVAFAPIVGLLLGMSSISVPWDTLLISVVLYIIVPVILAQLLRRHLL
                     KQGQAAFERAMQKIGPWSMAALLLTLVLLFAFQGEAIIRQPLVIAMLAVPILIQVFFN
                     SGLAYWLNKRAGEKHSVACPSALIGASNFFELAVAAAISLFGLHSGAALATVVGVLVE
                     VPVMLLVVRVVNRSKSWYERG"
     gene            5193..5396
                     /locus_tag="ACHKUI_003088"
     CDS             5193..5396
                     /locus_tag="ACHKUI_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599242.1"
                     /translation="MTDPKNLENRLHEKADPARAVSTGRVRYTLAELLADAEASGAYP
                     LPPEEREWMDAPSVGREWPNSED"
     gene            complement(5433..5657)
                     /locus_tag="ACHKUI_003089"
     CDS             complement(5433..5657)
                     /locus_tag="ACHKUI_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system ptaRNA1 family
                     toxin"
                     /protein_id="HFO6599243.1"
                     /translation="MTTQHIIEPGQAVHQAAAILSSLEYINQAEARSLGPLAEAVANA
                     FMVVYYQAETGRATQADFQEAMNALRQACS"
     gene            complement(5721..5969)
                     /locus_tag="ACHKUI_003090"
     CDS             complement(5721..5969)
                     /locus_tag="ACHKUI_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599244.1"
                     /translation="MTTSTYMAALAALDEAPREEVSQVFISPLGDQSTEVDAETETAA
                     FVSRPDPMRESETKPISIEVNGGRNERSAFAEAAGLYL"
     gene            complement(5974..7401)
                     /gene="trbL"
                     /locus_tag="ACHKUI_003091"
     CDS             complement(5974..7401)
                     /gene="trbL"
                     /locus_tag="ACHKUI_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFO6599245.1"
                     /translation="MRKKIALGGLMMVATMVLAEPAMAQELSSSGVMNDVLKRFHDAA
                     ATWGPAIESAASRLFWTLVVISMVWTFGMMALRKADIGEFFAEFVRFTIFTGFFWWLL
                     TNANQGMNIAGTIVQSLQTLGAQAGGLSNSNLGPSSILDLGFELYNRTVQATSELGWR
                     QMATALVMELMALAVLFVLALIAVNLLLLLASAWILLYAGVFFLGFGGSRWTSDMAIN
                     YYKTVLGLAAQLMAMVLLVAIGKEFINHYYTQISENMASQELAVMLVISVILLFLVNK
                     VPPMISGLVSGGGIGAAGGIGNFGAGAAVGAAVTAASMATGGAALAGKAVMGAAAGAA
                     GGASALQAAFQKASASMETGGDMSSMGSVVSSGGNGGGEAGTAGSSPFAQAAGFGDSG
                     SSSSGGGFAKAAKLATGTASELAKGVGSQVKQGFQERVSETTGGKLAASIRESMEPKE
                     ASQSGQFEGNSLGADSGPDSNEVRS"
     gene            complement(7412..7648)
                     /gene="trbK"
                     /locus_tag="ACHKUI_003092"
     CDS             complement(7412..7648)
                     /gene="trbK"
                     /locus_tag="ACHKUI_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion lipoprotein TrbK"
                     /protein_id="HFO6599246.1"
                     /translation="MKKIFFTTAATLLLLAGCEQERMPEPNAATCAPDAFQAALNEMR
                     SEANREAFTEECRAFQKAKQMRQWEFKPSPKDDY"
     gene            complement(7661..8440)
                     /gene="trbJ"
                     /locus_tag="ACHKUI_003093"
     CDS             complement(7661..8440)
                     /gene="trbJ"
                     /locus_tag="ACHKUI_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO6599247.1"
                     /translation="MKSKILAAKKTALAVALATGFITTTTAPVQAGIPVIDGGNLAQN
                     IMTAIESVAQTLKQIEQYQTQLQQYENQLQNTMAPAAYIWDQAQTTINRLIAAQNTLA
                     YYENQLGSLDRYLAKFQDVAYYRSSPCFNGSGGCTPAEKAAMEENRRLASESQKKAND
                     ALFQTVADQQKALKDDARTLERLQGAAQGATGQLQAIGYANQLASQQANQLLQIRTML
                     TAQHNAEAARIAAELDAEARGDARAEQMRTWTFRPSPADNY"
     gene            complement(8581..8769)
                     /locus_tag="ACHKUI_003094"
     CDS             complement(8581..8769)
                     /locus_tag="ACHKUI_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stabilization protein"
                     /protein_id="HFO6599248.1"
                     /translation="MANVLIRNLPAEVHRALKVRAALNDRSTDAEIREILTAAVQPLE
                     LEEIGREVGLSESDKKTE"
     gene            complement(9802..10683)
                     /gene="repC"
                     /locus_tag="ACHKUI_003095"
     CDS             complement(9802..10683)
                     /gene="repC"
                     /locus_tag="ACHKUI_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFO6599249.1"
                     /translation="MKKPKHDLTHVRHDPAHCLAPGLFRSLKRGDRKRCKLDVTYTFG
                     EDESMRFVGFEPLGADDMRLLQGIVALGGPNGILLTPEPTSETGRQLRLFLEPRFEAI
                     EQDGLVVRESLTKLLSETGMTDSGDNIKALKASLLRMSNVTILVTKGRRQAAFHLMSH
                     AFDETDGRLWVALNPRIAEAILGHRPYARIDMAEVRVLQTDPARLMHQRLCGWIDPGK
                     SGRVELDTLCGYVWPDEANAEAMKKRRQTARKALAELAAVGWVVNEYAKGKWEIKRPG
                     PTATAPVYRRNVPLLPS"
     gene            complement(10670..11497)
                     /locus_tag="ACHKUI_003096"
     CDS             complement(10670..11497)
                     /locus_tag="ACHKUI_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6599250.1"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     gene            complement(11502..11723)
                     /locus_tag="ACHKUI_003097"
     CDS             complement(11502..11723)
                     /locus_tag="ACHKUI_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6599251.1"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     gene            complement(11871..13073)
                     /locus_tag="ACHKUI_003098"
     CDS             complement(11871..13073)
                     /locus_tag="ACHKUI_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599252.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
     gene            complement(13378..14955)
                     /gene="guaA"
                     /locus_tag="ACHKUI_003099"
     CDS             complement(13378..14955)
                     /gene="guaA"
                     /locus_tag="ACHKUI_003099"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6599253.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15024..16490)
                     /gene="guaB"
                     /locus_tag="ACHKUI_003100"
     CDS             complement(15024..16490)
                     /gene="guaB"
                     /locus_tag="ACHKUI_003100"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6599254.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            16652..18028
                     /gene="xseA"
                     /locus_tag="ACHKUI_003101"
     CDS             16652..18028
                     /gene="xseA"
                     /locus_tag="ACHKUI_003101"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6599255.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFELQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASLIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(18097..26148)
                     /locus_tag="ACHKUI_003102"
     CDS             complement(18097..26148)
                     /locus_tag="ACHKUI_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO6599256.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLMLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNEQGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGDGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSSDALVAPVVVNGGLADETSLNNTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTARLYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGAVFKRPLLLKELSSTSGRTAIAEDNEDWAQFTQAQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADLTKFDRMNNVVKVKKGEEAVLRVTTKDAQ
                     GKPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQNDFLSTTLVIYGVTGAD
                     GTTTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQLTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGAINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVV
                     AMDDLVVQPLSGSAVTLSESGNTISGMTGADGTASFSLRQDNTPGYKMPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNSKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(26269..27246)
                     /locus_tag="ACHKUI_003103"
     CDS             complement(26269..27246)
                     /locus_tag="ACHKUI_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO6599257.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(27301..29478)
                     /gene="sinH"
                     /locus_tag="ACHKUI_003104"
     CDS             complement(27301..29478)
                     /gene="sinH"
                     /locus_tag="ACHKUI_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO6599258.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINFATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRLNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDLLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         6624 a         8323 c         7583 g         7018 t
ORIGIN      
        1 atcttaaatg tcttattttt cgtaatgtgt ataacaagga atagtgatga aatttaaaaa
       61 atgtcttctg cctgtggcaa tgttagcgtc attcactctg gcaggatgcc agtcaaatgc
      121 tgatgatcat gccgccgatg tttatcaaac cgatcaactg aataccaaac aagaaactaa
      181 aaccgttaat attatttcca ttcttcccgc aaaagttgcc gtagacaacg cccaaaataa
      241 acgaaatgca caagccttcg gcgcgcttat tggtgcagtc gctggcggtg taatcggcca
      301 caacgtgggg tctggcagca attccggaac gacggcaggt gcagttggcg gcggagctgt
      361 tggcgcggca gcgggttcta tggtgaatga taaaacctta gtggaaggtg tttctctaac
      421 gtataaggaa ggcaccaaag tgtatacctc cacccaggtg ggtaaagagt gccagtttac
      481 gacaggttta gccgttgtta ttaccacgac gtataacgaa acgcgtattc agccaaatac
      541 gaaatgtcct gaaaagagct aatgatcagg aggagtcatg aagaaagttt ttctttgcgc
      601 catcttagcc tccttaagct atccggctat cgcctcatca ttgcaggatc aactctcggc
      661 tgtcgcagaa gcggaacagc aaggtaaaaa tgaagagcaa aggcagcatg acgaatgggt
      721 cgcggagcgc aacagggaaa tccagcaaga gaagcaacgt cgcgcaaacg cccaggccgc
      781 ggctaataaa agagcggcaa cggcagcggc gaataagaaa gctcgtcagg ataaactgga
      841 cgccgaagcc actgcggaca aaaaacgcga tcaaagttat gaagatgagc tacgcagctt
      901 agagattcag aagcaaaaac tggcgctggc gaaagaagaa gcccgcgtta agcgagaaaa
      961 cgaatttatc gatcaggaac tgaagcacaa agctgcgcaa accgatgtgg tgcaatctga
     1021 agctgacgca aacaggaata tgactgaagg cggtcgcgat ctgatgaaaa gcgtgggcaa
     1081 agcagaagag aataaatcag atagctggtt taattaatcg atgttagtaa cttcaatcct
     1141 atgattcgtg agtataaaaa accctctgta gtaacagagg gttttgttca ttcatagtgc
     1201 agagtcaaat cattcccact cgattgtaaa caagcacaca aaacccttta gtgacaacaa
     1261 tttgcaagaa ctatcaggct cagtgccatg aaaaatacca tgaccagatc atgaggttga
     1321 acacggcttg cgccggcagc gttcacgctc ctggtcggcg atctcgcccg gctgcaatgc
     1381 tagctcagca tcggtcatgc gctccagcac gtcgcgtagg cggatggtgc aaggaatggg
     1441 cggcagctcg aactcatagc cgccggcgat catttcggcc gcaatctcct cggtgatgaa
     1501 aaacggctcg tcgtcttggt tcggcttctt catgcccttt cctcctggcg ctcatcctgg
     1561 ccgacagcgg ccagggcatc ggcttccgtc aatgcgtcct ccacgaacgc gccgtgctgc
     1621 ttcgcttcgg cctggctgac ctcggcccat atccgtttca cctgggccgc actgtacccg
     1681 gccagctcgg cggtcttgtt gatgctgtag ccggacttgc gcagcgcgac aacttgggcg
     1741 cggcgcttcg gatcagcacg gcggcccttg taccgcccgg cctggcgggc caactcaatg
     1801 ccttggcgct ggcgttcgcg cctgtcctcg tagtcgtcgc gggccatctg caaggccagg
     1861 cgaaaaagca tgatctgcac ggcttccagc acgatcttgg cgacgccctg ggcctcggcc
     1921 gccaggtcgg atagatcgac cacgccaggg acggccaggc gtgcgccttt ggcctgtatc
     1981 gaggccacca ggcgctcggc ctcgggcaaa ggtaggcggc tgatgcggtc gatcttctcg
     2041 gcaatgacca cctcgccggg ctgtaggtcg ccgatcatgc gcagcagctc aggccggtcg
     2101 gcgcgtgcgc cggatgcctt ctcacggtag atgccggcga cgtagtagcc ggcggccttc
     2161 gcggccgtag tgatcgcctc ttggcgttcc aagtcctgcg cgtcggtgct gacgcgcaga
     2221 tagacccgcg ccaccatcgg cgcggctact ggcttcgtcc tgcgcatact tgcgggctcc
     2281 tttgggcata ctcaaatgca cccatcttaa actggcaggc caatttatca catatcgatc
     2341 taaatgcgcc tagctcattt ttacagctta aatgagctat tgagcacact ccatatttcg
     2401 actattattg aaacatggaa acgataaatg ctgtagccgc cctggcggcc atcgctcaag
     2461 aatcgcgcct cgcggtgttc cggcttctcg tccaggccgg ccccgccggc atggccgccg
     2521 gaaaaatcag cgaagcggcc ggcatcccgc cgtcttcgct atccttccac ctgaaagagc
     2581 tggcacacgc cggcatggtc acgtcgcggc aagaaggccg atttgtgatc tacgaggcga
     2641 acttttcgac ggccactaac ctggtggcct ttctcacgga aaactgctgc ggcggccagg
     2701 tgtgcaacct gtcttgcacc acggaagccg ggaaggtgtt ggcatgagac ttcgccatct
     2761 ttccgacccc gattctttgc ccgctttgga caaatccttt gccatcgagc gcccggcgct
     2821 cgggctggca cccgacgccc cgccggtgcg tatcctgctg ctgtatggct cgctgcgcgc
     2881 ccgctcgttc tcacggctgg ccgtcgagga agcggcccgg ctgctgcaat tctttggcgc
     2941 agaaacacgc atcttcgacc cgtccgattt gccgttgccc gatcaagtgc aaagcgacga
     3001 tcacccggcc gtcaaggagc tgcgcgccct gtccgagtgg tcagagggac aagtctggtg
     3061 cagcccggaa cgccacggtc agattaccag tgtcatgaag gcgcagattg accatctgcc
     3121 gcttgaaatg gccggcatcc ggccgaccca aggccgcacc ctggccgtga tgcaggtatc
     3181 cggcggctcg cagagcttca acgccgtgaa caccttgcgt ctgctcggcc gctggatgcg
     3241 aatgttcacc attccgaacc agtcgagtat cgccaaagcg ttccaagagt tcgacgcggc
     3301 gggccgcatg aagccctcgc cgtactacga ccgaatagcc gatgtgatgg aagaactggt
     3361 gcgcttcacg gcgctggtac ggccgcaccg tgaagcactg acagaccgct attccgaacg
     3421 gaaagcggcc ggccacgtca tcgacgaggc caccgatctt tcatccatcg ccattgctcc
     3481 ccagccacta ccagaaagcg aaacatcatg accgaaagaa tctataacgt cctcatcctc
     3541 tgcaccggca attcggcgcg cagcatcatg gccgaagctc tcatcaacac gatggggcaa
     3601 gggcgcttcc gcgcctacag cgccggcagt cacccaaccg gcaaggtcaa tcccttcgcc
     3661 gtcgaaaagg tggagtcggt gaattacccg accgagaatc tgcgcagcaa gagctgggac
     3721 gagtacgcca cgcccgacgc accgaagatg gacttcatca tcaccgtctg cgacaacgcc
     3781 gccggcgaaa tgtgtccggt gtggcctggt cagccgatct cggcgcattg gggatttgaa
     3841 gacccggccg ccgtcgaagg cactgacgcc gagaagcgcc gggccttcga acaaaccttc
     3901 cggcacatga tgaaccgcgt ccgcctgttc gtgaatctgc ctctcaaaat gcttgaccag
     3961 acggccatca agcgcgagct ggcgaacatc ggcaagaccg agcaggaagc atgagcgccg
     4021 gcgcacaggc catcgccgcg aagccgcgcc aggccccgat gagcggcttc gagcgttacc
     4081 tgacgctgtg ggtggccctg tgcatcgtcg ccggtgttgc gctcggccag ggccttcctg
     4141 acgtgttcca ggccataggc cgcatggaag tggcccaggt caatttgccg gtgggcctgc
     4201 tgatttgggt aatgatcgtc ccgatgctgg tcaagatcga cttcggggcg ctgcaccagg
     4261 tcaaagagca ttggcgcggc atcggcgtca cgctgttcgt gaattgggcc gtcaagccat
     4321 tctcgatggc cctgctgggg tggctgttca tccgacaagt gttcgctccg ttcctgccgg
     4381 ccgatcaact ggacagctac atcgcgggct tgatcttgct ggccgccgcg ccctgcacgg
     4441 caatggtgtt cgtgtggagc cgccttacca acggcgaccc gcttttcacg ctctcgcaag
     4501 tggctctgaa cgacgccatc atgatcgtgg ccttcgcgcc catcgtcggc ctgctgctgg
     4561 gcatgtcctc gatctcggtg ccttgggaca cgctgctgat ttcggtcgtg ctctacatca
     4621 tcgtgccggt gatcctggcg cagctcttgc ggcggcatct gctcaagcaa ggacaggccg
     4681 cctttgaacg ggccatgcag aagatcgggc cgtggtcgat ggccgcgctg ctgctgacgt
     4741 tggttctact gttcgccttc cagggcgagg ccatcatccg gcagccgtta gtgatcgcca
     4801 tgctagccgt gccgatcttg attcaagtgt tcttcaattc cgggttagcc tactggctga
     4861 acaagcgggc gggggaaaag cactctgtcg cctgtccttc ggccctgatc ggtgccagca
     4921 acttcttcga gctggccgtg gcggccgcca tcagcttgtt cggtctgcac tccggcgctg
     4981 cattggcaac cgtggtcggc gtcctggtcg aagtgccggt gatgctgctg gtcgtgcgcg
     5041 tggtgaatcg ctcgaagagc tggtacgaac gaggttgaac atgagtagga aaaaactgag
     5101 gaaatgattg cagattgccg cgccggccgc ttcctggcgc attcgatcac tgacctggaa
     5161 cacctagtat ccatgcttgc tgaggtcgcc cgatgacaga cccaaagaac ctagagaaca
     5221 ggctgcatga gaaggccgac ccggcccgcg ccgtttccac tggtcgggtg cgctacacac
     5281 tggccgaact actggccgac gcggaagcat ccggggctta cccgctgccg ccggaggaac
     5341 gcgaatggat ggatgcgccg tctgtgggtc gggaatggcc aaacagtgaa gattgaaaaa
     5401 ggcggccagt tcgagtggcc gccctttcgt cattagctgc acgcttggcg caaggcgttc
     5461 attgcttctt gaaaatcagc ctgtgtcgcc cggcccgttt cagcttggta gtacaccacc
     5521 ataaaagcat tagcgacggc ttcggccaat ggcccaaggc tccgcgcttc cgcttggttg
     5581 atgtactcca aagaagaaag aatagccgct gcttgatgca ctgcttgccc tggttcgata
     5641 atatgttgtg ttgtcataat tcacctctcc agtaggtgtt gattgaaagt gccgggctgg
     5701 ttgtggccag cccggtgcgc ctacaggtac agccccgccg cctccgcgaa tgctgaccgc
     5761 tcattcctgc cgccattaac ctcaatactt atcggcttag tttcgctctc tctcataggg
     5821 tctggccgtg atacaaaagc ggctgtttct gtctctgcat cgacctcggt cgattgatca
     5881 cccagcggcg aaataaatac ctggctgact tcttcccttg gcgcttcatc cagggccgcc
     5941 aatgcggcca tgtatgttga tgtcgtcatc gtgctaactc ctgacttcgt tactatctgg
     6001 gccagaatcg gcgcccaagc tattgccctc gaactggcca gattggcttg cttctttcgg
     6061 ctccatgctt tcgcgtatcg aagcagccag ttttccgcct gtggtttcgc tcactcgctc
     6121 ctggaatccc tgcttcactt gagagccgac acccttggct aactcggagg ccgtgcctgt
     6181 ggccagcttc gcggccttgg caaagccgcc accgcttgag ctactgccgc tgtcaccaaa
     6241 gccagccgct tgggcgaatg ggctactgcc agcagtaccc gcttcaccac caccgtttcc
     6301 gccactgctg acaactgacc ccatgctgga catgtcaccg ccggtttcca tactcgctga
     6361 tgctttctga aaagccgctt ggagtgcact tgcacctccg gctgcaccgg ctgcggcacc
     6421 cataaccgct ttacctgcca gggcagcgcc gccagttgcc atactggccg ccgtcaccgc
     6481 cgccccaact gccgcacccg caccgaagtt tccaattcca ccggctgcgc caataccacc
     6541 accagacaca agaccagaaa tcatcggcgg aactttgttg accaagaaaa gcaagatgac
     6601 cgatataacc aacatcacgg ccagttcctg ggatgccatg ttctcgctga tttgcgtgta
     6661 gtagtgattg atgaactctt tgccaatcgc aaccagaagc accattgcca taagctgtgc
     6721 ggccaggccc agcacggtct tgtagtaatt gatcgccatg tcggaagtcc aacgacttcc
     6781 accaaagcca aggaagaaca caccggcata caacagaatc caggctgatg cgagcaacag
     6841 caacaggttg accgcaatca acgccaggac aaacaaaaca gccagggcca ttagctccat
     6901 gaccagagca gttgccatct gccgccatcc cagctccgag gtggcctgta ctgtgcggtt
     6961 gtataactcg aaaccaagat caaggatgct ggagggccca agattgctat tggaaagtcc
     7021 ccccgcctgc gcgcccagag tttgcaatga ctgaacaatc gtaccggcga tattcatgcc
     7081 ctggttcgca ttcgtcagca accaccagaa aaagccggtg aagatcgtga agcgaacgaa
     7141 ctccgcgaag aactcgccaa tgtcggcctt gcgcaaagcc atcatgccga atgtccagac
     7201 catcgaaatc acaaccagcg tccagaacaa acgcgatgca gcggactcaa tagctggccc
     7261 ccatgttgcg gcagcatcat gaaaacgctt gagcacatca ttcataacac cgctgctact
     7321 tagctcctgc gccattgcag gttcagccag caccattgtg gcgaccatca tcaagccacc
     7381 tagagcaatt ttctttctca tggcgaccac ctcaataatc atctttaggg ctgggcttga
     7441 actcccactg acgcatctgc ttggccttct ggaatgctct gcattcctcg gtaaaggcct
     7501 ctcggttcgc ttcgctgcgc atttcattca gtgctgcctg gaacgcatcg ggggcacacg
     7561 tcgccgcatt tggttcgggc atcctttcct gttcacatcc agccaacaac agcaacgtag
     7621 ccgcggtagt aaaaaagatt tttttcatct caccgcctcc ctagtagttg tctgccggac
     7681 tcgggcgaaa cgtccaggta cgcatttgct cggccctggc atcacctcgc gcctcggcat
     7741 cgagttctgc tgcaatccgg gctgcctccg cgttgtgttg ggcggtcaac atggtgcgaa
     7801 tctgcaagag ctgattggcc tgctggctgg cgagctggtt ggcgtagccg atggcctgta
     7861 gctggccagt tgcaccctgg gccgcgcctt gcagccgctc cagggtacgg gcgtcatcct
     7921 tcaaagcctt ttgctggtcg gcaacggtct ggaacagggc atcgttggcc ttcttttgcg
     7981 actctgacgc caggcggcgg ttctcttcca tcgctgcctt ttcagccggc gtgcatccgc
     8041 cgctgccgtt gaagcatggc gagctgcggt aataggccac gtcctgaaac ttggccaggt
     8101 aacgatccag gctgcctagc tggttctcgt aataggccag cgtgttttgc gcggcaatca
     8161 gccggttgat cgtagtctgc gcctgatccc agatatacgc ggctggggcc atcgtgttct
     8221 ggagctgatt ttcgtactgc tgcaactggg tctggtactg ctcaatctgc ttgagcgtct
     8281 gcgccaccga ctcgatggcc gtcatgatgt tctgggccag gttgccgccg tcgatgacgg
     8341 ggataccagc ttgcacaggg gcggtggtag tggtgataaa gccggttgcg agtgcaacgg
     8401 ctagagcggt ttttttagcc gctaaaattt tggacttcat ggtcgtactc cgtcgatgaa
     8461 aagaaggcat tgccttgacc aagcgttgtg cttgttctcg gcaacgtttc atcgaagagc
     8521 tacgccacta gctgatcggt attgcccttg gcgctgaggc ccggacttac aaacgtaaaa
     8581 ctactctgtt tttttatcgc tttcggacaa gccaacttcc cggccaatct cttcaagctc
     8641 tagcggctgt actgccgccg tcagtatttc gcgtatttcc gcgtcggtgc tcctatcgtt
     8701 gagcgctgct cgaactttca acgcacggtg cacctcggcg ggtaaattcc tgataagcac
     8761 atttgccata tcaataccct tttggtgtac ccgacaacca tgctatcaaa gatattaaaa
     8821 atagcaagac ctatactctt gcaaccggcg gtagccgatc agccccgcag ggcaggattc
     8881 ccgttgagtg cccccggcgc gaataaggga cagtgaagat agataaccgg cttgccggtt
     8941 agctaacttc acccatcctg cccgcatttc acccactatt gaaaatctag cgcatatcga
     9001 acgtaaagcg ttttatcgtc aaacaaacac cgattcttgg caacataacc gtaatcgtca
     9061 ccaaatcggc ctgcaatcga catagtcgct tcaaaatcgc tgctcaatcg gctttaaatc
     9121 ggaacctaaa atgccaacaa tcgacacaaa ccattcgccc cgatggggcg agttggacgc
     9181 caaaaaagaa gggcataacc cgcccctcaa gtgtcaatga acacaagaaa tttgcctccc
     9241 ctcccgtcat accactaagg cgacgtattg atacttgagg ggcaaccttt cctgatgttg
     9301 acaccttcac gcaggttact ggttatgggc gtccaacaca cacggctcat tcctgcttgg
     9361 taataataag aataataatc ttcttattat tcttaccgaa cgaatgggca ctgcttagcc
     9421 atttcacgtt tcgagccaag gtttcagtac agataactac aagtgcacac agcggccctc
     9481 cagcaccgtt agacgcagat tctgcttcag ctcatggaat aagggactcc gggttgtttt
     9541 cccctctcac aaggcgtttc agcggccact gaagccgcct tctcttcttg gcgttaaacc
     9601 atcggcctat ggccgattga taaagcccgc cagggcttta ttggcaaggt cagaacgcgc
     9661 cctcaaggcg cgagctgcac cgcgcatgat ggattatctt tagataatct tggatggatt
     9721 tcccaaactg cccgcaaagc agcgcaccgt ctgggttttc caaattggga cggggaacgt
     9781 tacgacggca gaaggggagc gttacgacgg taacaaggga acgttacgac ggtaaacggg
     9841 tgcagttgcc gtggggccag gcctcttgat ctcccatttt cccttggcgt attcgttcac
     9901 tacccaaccc acggcggcaa gttcggccag tgccttccgg gcagtctgac ggcgtttttt
     9961 catagcttcg gcattggctt catctggcca gacatagccg caaagcgtgt ccagttccac
    10021 gcgcccggat ttgccggggt cgatccagcc gcatagccgt tggtgcatca gccgtgccgg
    10081 atcagtctgt agcacccgca cttccgccat gtcgatacgg gcatatggac gatgccccag
    10141 gatcgcttcg gcaatacgcg gattcagggc aacccatagc ctgccgtccg tctcgtcaaa
    10201 agcatgactc atcaggtgga acgcggcttg ccgccgtccc ttcgtcacaa ggatggtgac
    10261 gttcgacatg cgcagcaggc tggctttgag cgccttgatg ttgtcgccgc tatccgtcat
    10321 gcccgtttct gagagcagtt tggtcaggct ctcacgaacc accaagccgt cttgctcaat
    10381 ggcttcgaaa cggggttcaa ggaatagccg tagctgtcgc cccgtctcac tggtcggttc
    10441 cggggttaac aagatgccgt tcgggccgcc aagggccacg atgccttgca aaagacgcat
    10501 atcatcggcc ccgagaggtt cgaatccgac gaaacgcatg gattcgtcct cgccaaaggt
    10561 gtaggtcacg tccagcttgc agcgtttgcg atcgccacgc ttgaggctgc ggaacaggcc
    10621 aggtgccaaa cagtgcgcgg gatcatgtcg gacgtgggtc aggtcatgct taggcttctt
    10681 cacgctgtct ccttttcacc ttgcactgcc gctccagtac agcaggcttc aacacgccgc
    10741 cgtcgtgccg tctaaaccag agttcttgaa aaggtgcgcc atagttggcc ttgctgacgc
    10801 caaagcggac aaaataccca cgctgacaat catcgacccc cagtatctcg gcctcgcctt
    10861 gcgtcatgcc ggataggtac gattgccaac ggatgttatc aaccagtacg gacgatccac
    10921 gactggcctg ctgttgatcg cccgacccca tcatggctgc gcttttactc gcgtggtgca
    10981 gaaacacaat ggagcagcct gtatcagcgg ctatggcctc catgtgccca acaacctgcg
    11041 ccatcggccc gctagcgttc tcctcctcaa tgtggaagcg ccgcaaagtg tccaagatca
    11101 tcaagcgacg accttcagcg gctcgtttga gagcatcgaa ccagctagca gccatgatgt
    11161 ttgggcattt accgatcaag ggttcaatga gcaaaccatc agccacggct tgccgttccg
    11221 ctgcgctgag gtgtgcccca agggcgtgca gacgatggtg aatagcggcc ggtggatctt
    11281 cagcaggcag atagaccact tgcccggtgg gaaactcgcc tatttcaagt aaatcaggcc
    11341 cgcctgcaat ctgtgcagcc aattgaaggg ccaacatgga tttaccagcc ccaccaggcg
    11401 acacaagagc accaacagtg ccagcaacca tattaggcag aacataatca atgggtggcg
    11461 gcaattccgt gaaagccgcc ataagatcaa gagccatata actagccctc tatagggcca
    11521 ggtcgcgcag cttgatcacc cgatgattta cgtgcctcaa tccactcttc gacctctgac
    11581 aaatcccagg cgacatttct actggttaac gcgatacggc gtggaaaatc ccctcgctgc
    11641 tccaggttat aaattgttcg tgtcgaaagc gggatcatct ccaggagctt cttcctgttg
    11701 atgagggtct ttatattttg catgggtcaa tacctctcaa atgaatagtt gctattcatc
    11761 gagtagctgc gcgaagcagc taatcggtaa ttgacctgcc cgatgccaat ggcggcctta
    11821 tttataaatt cacggcatca acttcaatgc tatcaaagat agcaaaaaca tcaaccctta
    11881 ctgcctcccg aagtccactc atcaatcata tcggcccaat cctgcaacat cgccgcccgc
    11941 tgctcgcggt actcagcctt gttgtagaca gccctcacgc ccttctgctc gtgcgccagg
    12001 cacttctcga tccagtcggt gttgtagccg gcctcatgca acagcgtgct ggctgtgcgc
    12061 cgcaaatcat gcggtccgaa cttggtaagt gacttcccat ctttctgcgc cgccttgtaa
    12121 gtcagcgtca gcacctggtt aagtgtggca gcgctcatcg gcgcatccga gtcgtaccgt
    12181 gatggcaaaa cgaagtcgga accaccggca aaggttttca tggcaatgaa aatatccaga
    12241 gcctgctggg acagaaatac caggtgcggg ttacggcgtt tcatccgctc ctttggaatc
    12301 gtccacaacg cttcgctgaa attgatctcg ctccaggtcg cattggtcag ctcgctcttg
    12361 cgcaccatcg tcagcaataa cagcttggcc gccgcacggt ttgttgggct tgtgcccact
    12421 cgctccatgt actggtacat cagcccaatt tcttctggcg tcaacgctcg gtcacgtggc
    12481 tcgaatcgag cgatgcttgt tgggcgcacc agttctgccg ggttttcgac cttctgccca
    12541 cgctcgatgg cccagcgaaa tacctgcaac acgatttcac gcacatgaac ggcggtggcc
    12601 ggtgcgcctc gctcaacaat ggcatcagcc agcgcccgca agtcttcgtg ggtgatctcc
    12661 accagcttct gattgctgaa tttcggcttc agctcacgct cataaaccga gcggcgcata
    12721 tcgcgggtgg agtcggccat ctggtagcca cgcagccact tctccgccca ggcaccgaac
    12781 gtctctgcat ctttcacccg cgccttgtct cgggctttct ccttggccgg cgacttgccc
    12841 gccgcaacca tcttcttggc gtcacccaac agctcgcggg cttcggccag ggtgatgcca
    12901 ccgacaccat aacgcccaaa ggtgatggtc tcctgccgac cgttgattga gtagttgtaa
    12961 cggaacgaga tggagccggc tggagtcact gccacataga gaccttcccg gtcattcact
    13021 ttgtagagtt tgtccctggg cttgagattg cgcagcttgg tatcggtcag catgtacctt
    13081 gtccttcttc gtctccgata ccatgctgaa aaaatctcga atttatcgta ttttttctta
    13141 cgaaacagta acttatagaa tattaatacc atgaacacac aaaagaacgc agcatggtat
    13201 cggcatcaat catggcatcg ccaaaccata ataccagctt taatgccatg ctcaaacggt
    13261 gcttggcgct tcctcccgtt gccagatcgt gccaaacaca aacaaaaaaa agcccgcaat
    13321 tacgcgggct tagcggcatt tcatgccatc aggtgcctgg ctgtgccaga cgcggaatca
    13381 ttcccactca atagtcgctg gtggcttgcc gctgatgtca tacaccacgc gggaaatacc
    13441 attcacttca ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg gcagatgtgc
    13501 ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc agagagacaa cccagtcata
    13561 cttacgacca tcgcccatta cgccaacgga acgtactggc aggaagacgg tgaacgcctg
    13621 gctgactttg tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg
    13681 gcgcagcagg tcacaatact ctttcttcac ttctcccaga acacgaacgc caaggcctgg
    13741 tcccgggaac gggtgacggt acaacatgtc atacggtagg cccagctcca gaccaatctt
    13801 acgcacttcg tctttgaaca gctctttcag cggttcaacc aggcccatct tcatctcttt
    13861 cggcaggcca cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc
    13921 agcagattcg ataacgtcag ggtagatggt gccctgcgcc agccacttca cgtcttccag
    13981 tttcagcgct tcttcatcga atacttcaac gaaaacgcgc ccgatgattt tacgttttgc
    14041 ttccggatca ttttcgccag ccagcgctga caagaagcga tcttctgccg gtacgtgaac
    14101 aatattcaga ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg
    14161 cagcaggccg ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcaacag
    14221 cattgcggtt acggaggaat ccacgccacc agagaggccg aggatgactt tatcgtcgcc
    14281 cacttgctcg cggatgcggg ctacagcgtc gtcgataatt ttcgctggcg tccacagggc
    14341 ttcacactgg cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg
    14401 agtcacttcc gggtggaact gtacgccata gaagcgtttt tcttcgttgg ccatgatggc
    14461 aaacgggcag ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt
    14521 atcgccgtgg ctcatccaga cgtccagcag cggtttacca tctgcggtca gcgcatcttc
    14581 gataccgcga accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg
    14641 ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca tgccatagca
    14701 aacgccgaat accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc
    14761 ttcggtggtg ctttccgggc cgccggaaag aataatgccg cttggattga agtcacgaat
    14821 ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa acacccagct cacgcacgcg
    14881 gcgcgcaacc agttgagtgt actgagaacc gaagtccaga atgaggatgc gatgcttatg
    14941 aatgttttcc gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc
    15001 gacatgaagt cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta
    15061 atggtcacgt cgtgaacgtg gctttcctga atacccgcac cgctgatacg tacaaactcc
    15121 gctttagtac gcagttcgtc gatagtacca cagccggtca ggcccataca ggagcgcagg
    15181 ccgcccatct gctggtgaat gatctctttc aggcgacctt tataggctac gcggccttcg
    15241 ataccttccg gcaccagttt gtcggcagcg ttgtcgctct ggaagtaacg gtcagaagaa
    15301 cctttggaca tcgcgcccag ggaacccata ccacggtaag atttgtaaga acggccctgg
    15361 tagagttcga tttcacccgg agattcttca gtacctgcca gcatggaacc taccatcacc
    15421 gcgcttgcgc cagcggcgat agctttagcg atatcgcctg agaaacggat accgccgtca
    15481 gcgataaccg gaatgccggt accttccagg gcttctactg catcagcaac ggcggtgatc
    15541 tgcggaacac cgacaccagt cacgatacgc gtcgtacaga tagaaccagg gccgataccc
    15601 actttaacgg cactgcaacc ggcttctgcc agcgcgcggg cacctgcagc agttgccacg
    15661 ttgccgccga tgatttgcag gtcaggatat ttagcgcgag tttcacggat acgctgcaga
    15721 acaccttcag agtgaccgtg agaggagtcg atcagcagaa cgtcaacgcc tgcggcaacc
    15781 agcgcatcaa cacgctcttc gttaccagcg cccgcgccaa ccgctgcacc tacacgcaga
    15841 cggccttgtt cgtctttaca ggcgttcggt ttacgttccg ctttctggaa gtccttcaca
    15901 gtgatcatgc cgatcagatg gaattcatca tcaaccacca gcgctttttc aacgcgtttt
    15961 tcgtgcattt ttgccagcac cacttcacgg gcttcgcctt cacgcacggt taccagacgc
    16021 tctttcggcg tcatgtaaac actaaccggc tggctcaagt cggtcacaaa acgcacgtca
    16081 cgaccggtga taatgccgac cagttcgttt tcttcggtaa caaccgggta gccagcaaag
    16141 ccgttacgct cggtcagttc tttcacttca cgcagcgtcg tggttggcag tacggtctgc
    16201 ggatcggtta ccacgccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt
    16261 tcaatggaca tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc
    16321 aggcgcgctt ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata
    16381 gttttcgtca gctgagtgct gaggtcagca gtattcggca gaacggtaga atgagcagga
    16441 acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc
    16501 tcgaccagag tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc
    16561 tacccctttt tgcaaaaaat gcttgctatc cccggagggc gggttactat cgactgaata
    16621 acctgctgat ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt
    16681 taccgttagc cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tggggcaggt
    16741 gtggatcagc ggcgaaatct ctaattttac acaaccagcc tccggtcact ggtactttac
    16801 gcttaaagac gataccgcgc aggtgcgctg tgcgatgttc cgcaacagca accgccgggt
    16861 caccttccgc ccacagcacg ggcaacaggt tttagttcgc gccaatatta cactctacga
    16921 gccgcgcggc gattatcaaa ttatcgtcga aagtatgcag ccggccggtg aaggattgct
    16981 gcaacagaag tacgaacagc ttaaagcgaa gttgcaggct gaaggtttgt tcgagctgca
    17041 atacaaaaaa tcactcccct cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg
    17101 tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc gatccttctc tgccggtgat
    17161 catctacccc accgccgtac agggggatga tgcgccgggg caaatcgttc gtgccattga
    17221 actggcgaat aagcgcaacg agtgtgacgt gttgatcgtt ggccgtggcg gcggttcgct
    17281 ggaagatttg tggagtttta acgacgaacg cgtagcgcgg gcgatttttg ccagcctcat
    17341 tccggtagta agtgccgtcg ggcatgaaac cgatgtgacg attgccgatt ttgttgctga
    17401 tttgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg agccgcaatc aacaggagtt
    17461 actgcgtcag gtgcagtctg cgcagcagcg cctcgaaatg gcaatggatt attatctcgc
    17521 caaccgcacg cgtcgtttta cgcagatcca tcatcgctta cagcaacagc atccgcagct
    17581 ccggctggca cgtcagcaaa ctatgctcga acgtctgcaa aagcggatga gctttgcgct
    17641 ggagaatcag cttaaacgcg cgggtcagca acagcagcga ttaacacggc aactcgtcca
    17701 gcaaaatccg cagtcgcgta ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg
    17761 tttagcagaa accctgcgcg cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt
    17821 aacacacctc gaagccgtaa gcccactgtc aacgctggcg cgcggttata gcgtcaccag
    17881 cgccgctgat ggcgcggtgt taaaacaggt taagcaagtg aaagtgggtg agacactgac
    17941 cacacgactg ggagatggtg tggtgattag tgaagtcagc gccgtaacaa aaacccgcaa
    18001 atcacgtaaa aaaacgtcta atccgtagac cggataagag gcgccagcat cgcatccggc
    18061 aataactccc aggctactcc agatccgcaa tgctggttac tggaagcagg tcaccagacc
    18121 cggtttattt attagcgtga tactacaatc ctgtgctccg gtaccttcgt ccatcccaca
    18181 ctgttgagag gacaaataag gaaaactggg tgaagacggc catcccagtg aattaaaaac
    18241 agtatgcaac aactctaatt cattgtatgt cggtgcattt tctatgctgc cacactgcct
    18301 tgcgatatcc catttactgg catcaatgat atgtgcagaa gcccagtttt cgtagttaat
    18361 aatcggtgag gatacgggat tagccccgga cggcaattcc gtcgttaaca acggtcgatg
    18421 tagcgattta ctattcacca ctaccgtgtc tgccatatgt ccccaaaagt gcgccgatga
    18481 aacatttggg ctggttggca ccgtaaaaat agcgtccagc gtgtctgtcg cggaagcgta
    18541 attagccaga gtaaccgtca gtggcatttt ataaccaggg gtattatcct ggcggagcga
    18601 aaaacttgcc gtcccatccg cgcctgtcat gccactaatg gtattaccgc tttcactcag
    18661 cgtaacagcc gaacctgata acggctgaac gacgagatcg tccatcgcca ctacattgcc
    18721 atacaacgtt gccccagcgt tacgcggcga tgcctcacca cgtttcaaag taaattcaac
    18781 attcggcacc gtattcccgg cgctgtcttt caccgtcacg gtaacggaca tggcttcgcc
    18841 ttttttcact ttcgccgcct gagtggctgc atcaaacgct gttgaggtca gtgtgacgct
    18901 ggaaaccgtt gaacgcgcgt tagccaggca aggctgaaat gattttgttg aacccgtata
    18961 cagcgtgcca tttctcaaat caaccgactg gtctttacca tcggcggatt tatcctctgc
    19021 ccaccaggtg taatacgcag gccagccagt ttcagtgaca aacgtgttat tcggataacg
    19081 cgcagacaac gaacttaaat cattgaatag tggctgcctt gctacttcac aagggctcac
    19141 actcgcatct gatttctgtg ttggggtaaa gtacggccag tcttcattat tgttattgat
    19201 cttgccgacg ccagaagtcg atgtcagttc actccacaat ttcgggcgtt caaataccgc
    19261 accattcact gccgtcaagg ttgctggcat atgcccccag tagttagcct tcgcagaatc
    19321 cgggctggtc actaccgtga atatcaggtc ctgattagcc gtaacctgcg gatttcgcgc
    19381 caatgaagct gtaaacggcg ttaccagacc aacggtgtta ttttgggtaa cgttgaacgt
    19441 tatcgtgcca tcactgccgg tgaagccgct ccatgccgac gtattctggt tgaaggagat
    19501 acgcgccgat gtcggggtga cttcatctaa aatgagatca tctacggtat tggaggatac
    19561 agagatccct gaacggcttt tcgcctgcac gcgcttgagc gtaacacctt cgccgccaac
    19621 aggattacca ttggcatctt tgatcgtcac cacgatggga agcatctcac cttttttcac
    19681 agatgacgtg ggggtaccac cattgctggc agttcggccc tcatcatagg cagtcgatga
    19741 cagcgtaagg cttgctggtg ccgaatgtgg ctgcgtcaga cagagcatta aagcgtatcc
    19801 atttatagtc gtcgtcgatt gtcccgtttt caaattaatg gcgccgtaat accacgtcaa
    19861 cgcgtcagtg gtgctcattt cataagtcca ccaatttcca cttccggcag ttataggcaa
    19921 ccccaaatct gtcagtattg tcccgcctgg atgatcatta tatagcccct gtaactcatt
    19981 gagacgcggc tgatactcaa ccgggcaatc cggcgtaact ttattggtat aaaaaaggta
    20041 tgaccacgac tcattattat tacttaccga ggaccccaag gtcgcttctg ctgccagtag
    20101 cggtcgttta aaggtcaccc ccgccctact ggtaaacgtg tcgggcatat gaccccagta
    20161 ttttgctttc ggtgagttcg gactggttat cacagtgaaa ataacatcca tcgtggcact
    20221 ttttgtggtg tcgcgcatca atgttgcagt gagcggagtt gccagcccat aagtctcgtt
    20281 ttgcgtcaca tcaaaagtcg ctttaccatc acttcctgtc gtcagaagtg cctgtgcccc
    20341 attattatag agtgacatgt ttactccggc aggggtgacc ggcacgatag taagatcatc
    20401 atacgtagaa tctagcctgt tttttgcacg atccaacgca gtcccgcgta tgagattaaa
    20461 gtttgcacct ggcaacagat ttcctgcgct atcccgcaca atcaccgtca tccggatcgc
    20521 cgtacctttc gccgccgttg cactagggcg accatcgttg ctggccgttt tctccgcatc
    20581 cagtgccgtg gaggtcatct ctatagaagc tgccactgca tgagggttta ccagacagga
    20641 cacaaatgcc acggtgtttg ataatgccct ggagcttgat ccattcgata aattaataga
    20701 ctgatcgtac cagacgttcg cgtaggcaat tttatcgtaa gcccaccagg tattagtcag
    20761 cggaaggcct aaatcggtcc ctatcgcacc attaggatgt tcactgtata actcttgaaa
    20821 ttcgcttaat aacggttgat aaggttcgcc gcagtctgat ttgctgacat cattttttgc
    20881 tgccgtattc aaagaaagcc atgattcatt attagccatg gttgactgcc ccgctggatg
    20941 ctcggcagta agcaatggcc gcttaaaggc aattcccgac cgggtagtaa acgtttcagg
    21001 catatgcccc cagtaacttg ccagtggcgt atccgggctg gttatgacgg taaacatgac
    21061 cggtaatttg ttggattcta tcgtcagctg atacaactgg gcgtacatat tggttaccag
    21121 gcccatagaa tcatcttgtt ttaccgtaaa ggaggtagtc ccgtccgcgc cagtcacccc
    21181 ataaagcagg cttgaagaac tattcatcct cgcagaagcg cctgccgcgt taattaccgt
    21241 aatcgactta tttatcatct cggcattttg acggttcttc gagccttcac gtctgagaat
    21301 aaaggggaca ttgggcacgc tatttccctg cgcattttta gtcaccacgc gaatcaccgc
    21361 gtcttcgcct tttttcactt tcaacgcact caaggtgtcg tcaactttga caaattgcgc
    21421 cgtatcattc gcctcaacaa tgatctgact ggcaacggcg acaggcgtgg tccggcagct
    21481 cagtaatgca ccaccagatg agggcatctg cgtttcatct cctgtcttca gcgaaacgac
    21541 atcatacgta aaggtttgag tgaacgtatc tgcaacggta ttcgagatat agaacctcga
    21601 cactggccag ccaatagcgt catgtaatgc gctactcccc tggtgcgcat aaatggccct
    21661 aagttcattg tcagttggaa catactccgc gccacaacca tttatcgagg tatcgttagc
    21721 ctggcccgac gggaacgtag accaggcctc cccctcttcc accagggtac cgatactggt
    21781 ggtagcacta ttctcgtccc tcagcaaagg ccttcgataa ataagaccgt catcgcctgt
    21841 cgccgtttca gccatatgcc cccagaattt ggcttttggc gaatccgggc tggtaagcac
    21901 cgtaaagaca acgggaagcg tcgatttagt gctgctggag gaatccaacg ctgccgtgag
    21961 ttctgttttt agcccggtag tctggtcttg cttcaatgtg aaagtggtgg tgccatcagc
    22021 ccccgtcacg ccataaataa ccagggtcgt tgaaagaaaa tcattttgag tatttcccca
    22081 ggcatccgtt accgccagtg aagcaatcga cgtcgtagtt gacacattgg ccctgttcac
    22141 gctggtgttt cgtttgaggg taaatgcggt atttcccaca ggcttaccct gcgcatcttt
    22201 agtggtcact cgtaatacgg cttcttcacc ctttttcact ttgacaacat tattcatacg
    22261 gtcaaatttt gtgaggtctg cagcttcaag aacgatttgg cttgccttaa caatcggcgt
    22321 cgtctggcag gtaagcagtt cttttgtggc agatggaatt atcgaactgg tgcctgaact
    22381 gagattgacg gccttgaagt cacgctgttc caggctacta ctacctgtcg tgctggaaag
    22441 gtaacttgag gcgacgggcc agccctgaac cgttttcatc gcatttccgc ggttggcttc
    22501 atacaatgac tccagcccgg cctgagaggg aacatattca ctaccacaac cattagagga
    22561 tgtgcttata gcctgagctt gggtaaattg cgcccagtct tcattatctt ccgcaatcgc
    22621 tgttcgaccc gacgtactgg ataactcttt caacaataat gggcgtttaa agaccgctcc
    22681 atctgcagcc gtcactgttt caggcatatg cccccacatt ttcgccttat cgctgtccgg
    22741 actggttaca acggtaaaaa tggtatcgag ggtcgctttt ttcgttgtat ctgaatagag
    22801 cctagcggtc agcgacgttt tcgtgccatg ggtatccggg cgggtaatag tgagaatttt
    22861 agttccgtcg ctgccggtca tggcgctgta tgctgcagcg gtattattaa gcgaagtttc
    22921 gtccgccagg ccaccattga ccacaaccgg cgcaaccagc gcatcactgc taccagcaac
    22981 gtgtttttcc tctgagcggg tataaccatc gccacgtttc agggtaaagg gtatatcacc
    23041 caatggattg ccctgcgcat cttttaccgt cacctttacc tgtagcgttt cgcctttttt
    23101 cagcttcgcc gcatttgcag ccgcgttcca ctgcgccgga tccaccactt ccagcgtaat
    23161 gctactggca ggattgttag ccgtcgtcag gcaactgaca taaaccgccg tgctaccgcc
    23221 aacggtccgc gccccatcgt tgagggctat ggtgtaataa tgcggtacct gatctgcggg
    23281 cgaactgctc cagtagggct gacgctgcgt tggccagccg tgtactgtct gtacaccgtc
    23341 accgttattg gcatcataga gcgcgctaag ctgagaacgg cgtgggagca tattggcgcc
    23401 acagccacct ttactggtgc tggaggcgtt ggcctggctg actcgcgacc agacttcgtt
    23461 attttccgtt aatgtgccgt ccggactgaa cacttccgca gccagtcggg gtcgggcaaa
    23521 ggttaaagca tcgacggtaa tcgtctcatc catatgcccc cacatctgcg cgccaaccgc
    23581 atccggactg gtgagcgtag tgaaaatcac attgtaatta acagtattgg gaatatagga
    23641 gttcacaggc acaatattta gcggcgtcga taagcccaca ccttgcgatt gcttaatttc
    23701 cacagtcgca aaaccctgcg catccgtagt accggcatag actaaagacg gttgcgatgt
    23761 tccgtagagt gtgccattca tctcgattgc gccgcgggtt ggatcatcaa aacctgtagt
    23821 ctgcccctga cgatttttcc ccatatcttt agtaatggtg aacgcggtat acggcacggg
    23881 tgcattattc aaggcattaa aggtgcgtac cgtcatggtg atcgtgtcac ccactttggc
    23941 ctgcgctctg ctgcctgcgg aaacatcacg atcggtggtc gcggcaatct gggcgaccat
    24001 ctcattgccc gaacaagaga gatagttttg cttaaagccg gaataggtgt cgatgttgcc
    24061 cgtggccagg tttaccgacg cgtgcgacgt ctcttccacc gcactaagat acgctttttg
    24121 caacgttggc cagccatatt cggtgccgat ggcattggct ggatgagcgg caaacaaact
    24181 gtgcaatgaa ctttggctcg gaatatgacc taaaccacac tcagcctgca tactgctatt
    24241 ctgatcaaac agcgcccagt cttcgttatt ttcccgcacc gatccctgtt cgttgtcggt
    24301 ttcatccgcc agccgtgggc gtttaaagat attgttcgct tcaataatgc ccaacatatg
    24361 gccccacatc cgcgctttgt cggtatcggg gctggtgatc accgtaaaaa taacgtcttt
    24421 ctcatccgag gcagtaaaat cactgcgcat tctcgcggtg atgtgagttt tcacccccac
    24481 gccgcctggc tgggtcagcg tcagagtggc ctctccgtta gcatcagtga tcccttcata
    24541 ggtatgggcg tcaactttgc gaacgttact gccgccatca atttgtaccg gatgcgtctc
    24601 ccacgcggcg tcagtttgat tcttacggtt aatgccgtca tccagatgca gcgaaaagta
    24661 gtagtaagcc agcggctgat cgttgttctt gctatcggta atcgtcagac gtaaagaagc
    24721 ctcttccccc acctttactt tcatcgcttt tatcgtcgaa tcatagctgc ccggcgtcag
    24781 taccagcttc ggttcaacat cgggcgcttc tttatcgaca cagctcacaa taaacgtcgt
    24841 atcgtccggt ttttgcgctt cgctgcggtt cgaaacatcc gccgcatggt gctggccgct
    24901 catccccgcc agtgatgacc agtagaaatt accctgcatc ggccaaccag ccacatcctg
    24961 cactgacgtt ggcaccaccg tcgccagcgc accgaactga cgcattccgg gcaaaatgtc
    25021 gcaatgttta tccgcgccgc tccaggtaaa ggtggaccag gtttcattgt gatcgttaac
    25081 cgtaccgcct tcattatcga cttccgccgc cagttttggt cgactaaaag tgtaatcacg
    25141 ggccttcagc gtatcgggca tatgacccca cattgtagcc tgcggaacat ccgggctggt
    25201 gaggacggta aagatcactg ccgtttcact ggcctgagca atacccgcga ggctcgcgac
    25261 caacggcgtt tttacaccag ggccgttggc ctgggtgacc gtaaccgtgg caacaccatt
    25321 cgcatcggta gtaccacgat attccgtcgc ggtggtggtt aattcggtcg tgcccagttt
    25381 tacaggcgca gtattgttca ccacaccctg gcggttttca gcatctttgc gtttgataac
    25441 aaaaggaata ttccccacaa cattaccgac gcaatctttg gtggtgaccg ttaacgtgat
    25501 actgccccca accgtcattt tcgccgcacc tgaggattcg tcatagcccg cagcgctgac
    25561 ggaaataagg tcaccctgcg cgggcgcaat cttcagcgtc actttctgat cggcgagcat
    25621 cagaacactt tcatagggat agcccgtcga ggtgagtaaa tataacagcg gcatggtctg
    25681 ctggggatca ggctgcgccc aggcgaccag cgaacgcccg gcaagtccct gtgcacaaaa
    25741 cgagcgatct gcgactggct gtgggtccag taaggtgtcc ggcgtggcgg catccgccca
    25801 aactaaagat acagccggtg gcgtttggtt ttcccaacgt ggggtggcaa tgatatcgcc
    25861 ttctctgtct tgcgggttgg tgacgagcaa tttagagacg ctaacatccg cgttgaattc
    25921 actcggttta gcggtaaacg ctacctcatg cgtctttgta ggatcgagaa agacactgcc
    25981 ctggtagacg ggtatgctgg cgctgtcggc ggtgggcacg gtaccgttaa attcgccgac
    26041 cgtatctgaa gagtcttgcc atgcagattc tgcccatgac cacccaggca gcattgcgct
    26101 tatcagcaat gcgcataaca caaggatttt attcagggtc agggacatga cagaaactcc
    26161 accggttgtt aaacggacgc ttgtcgcatc aacatatttt gcttaatccg ggattaagca
    26221 aaatatgcgg aaacagcatt cccttgcggg aatgctgctg agtacgcgtt atttgtgttt
    26281 gtaaataatg tgaagtttgt aaccctgtac cccgtcgccg ttggtcggga tggaaagtgc
    26341 ctcttttcca tcgcgcgcag ggccgttaag gttggttctg gcttcgtcgt tggtcgcagg
    26401 gatgaccaaa ttgttattat cgaagcttga gttaacgatg ccacccgaag tgccaagctg
    26461 ttggctgctg ccagaaagta cccaacggaa gtcataattg gcggtgacgt cagcatcggt
    26521 acccgcatca aacttgccat ttttgttctc atccgagtac agtttggcca cgtaggtttt
    26581 gccaagttcg attggcgtgc taccgttgat aaggttgata ctggtaccat caacaaagat
    26641 ggcgactttc aggttctggt taaccaccgg ccccggtgca acgttatcgc tgtcgcttcc
    26701 gccgccgctg gcagtagaaa cgtcataaag atgcagcgca gtcccgacgt ttggcgtacc
    26761 ggtctgcgtg gttggcgtaa tttcaatacc gatataacga ccacggtcgg catcggtaag
    26821 agtgtaactg gtcaccttcg ttaccagctc ttttggatca cccccctgcg catccttgta
    26881 gctcatccag cggatagtat cgatagtttt cgcgttatcg cccaatcccc caagatccac
    26941 gtcgccttcg gtatcaccaa tttcccaact tacggttaat ttatcacccg agtaaagacg
    27001 cttgtccgtt tcactgcctt gcgggcgagc gccttttaga tcagaagtca acgttacacg
    27061 gtcggcatct gtcgtagcac ttcccccttc cgaatagagc cacggaatgg tcccctggaa
    27121 aatattggtt tcgccgctgt cgacccaggc actgtcttca gccagcgcca caggtgccac
    27181 acaataacct gccagtgcca gcgctaatgc gatccgggtc agaccgcgtc ttttatcctg
    27241 cttcattgat gctctcctta atgaaccttc agcgactggc cgactggcct tacgccaatc
    27301 ttattttttg gtatacagca cgcgtaaacc gtatccctgc aagccatctc ccgcttgtgc
    27361 gccataggtt tgcgccgctt cacggttagt ggccggaatc acaatgtcct cattggaagt
    27421 gttcgcctgt gcacccactt caccattgat gctcttgccg gtaaattccc acttgtagtc
    27481 gtactgcacc atctcttcgt cagtcagttt ttcgacctgc tggaagacac cgtctttatt
    27541 gttgtcgcgc cataaaacaa atcgataaat gttgttgagc ggcaacggtt ctttatcttt
    27601 acctatcagg ttgctgggtt tagccgcccc cacacgatag ataaaagcat ttagcccacc
    27661 aatttctgcc ccgaggaaag tgacatccac atagttactg tcatcgtagg gcgcggcttt
    27721 tgctttccag cgataggtgc caggtaaggt actggagagg gtgacagtgc ccttttcttt
    27781 atcttgtttg gcaataaaaa cgcaggggtt atcggtcgag cagggcttgt tggttaacgt
    27841 cactcgtttg tcctgcgcgt cgtaaagctg aggcgtaagg ttatcttccg gcaacgcatc
    27901 gccgttgcgg tcggtgttat cgccaaagct gtcggttaca tcgagatcca tcactacacc
    27961 atcaacgcca tctgcctgtg cgctggcttc gtggctgtgg ttgttctctt ctggcacgtt
    28021 cgcgatgtta ttaacaatca actctatttt acggtcctgc tgaacggtga tttccaccac
    28081 gccagagttc tgtcggttgc ccttttcatc ttccagcgcc agacgtactc gccaggtatt
    28141 gagtttttct tgttcggctt gatcggaact gtactgccag gcaggcagca ccaggttcca
    28201 gtggttattg ttaccctctg tcagagtatt ctctttgata accggcatca ccagcgtgcg
    28261 ggcggcattg tttttcgcct gccaggaggc cgcaacgatt ttgtaatgat gattaatgag
    28321 tgcgttaatg gtccacttac atttttccag accgacggcc gcttcgggag tgtccttaat
    28381 gacgcactca gggtgctcgt tggtggcatc cgctttcaac cacagggtga catcaagcgg
    28441 atctttttct ttgtattcca gaacaataaa gttgttacgt tcaacaaagt catgacggct
    28501 acccatgagc gagtgagccg cgctcacttt gtcaggatca agcaggtctt tgagcgggac
    28561 gccaaactgg tagttcagcg ttgcgttaac agaaagatcg gtattatcgc cagtgccagc
    28621 tttgaaatct gtaccgacaa caatgagcgg aacgggctgg tatttcacac ccagcgtgac
    28681 agcgaaggga tctttctcca gactatcggt accaaaaagc gcgacttcat tcccataata
    28741 ttgttcgaag acaatttttc cgccaagctg cgggtaagcg ggtagccagg cttcagcgcg
    28801 aatatcccaa ccgcgcgcag ggcgttcttc ataaaagtcg aaatcttcag agtctttcca
    28861 gtcagaaagt gggtgataat agttgcctga gaattttaaa taatccgtcc aggcttcggc
    28921 gcctaaacct aaacggcgat gtccacgggt aaagtcataa tcataaaata tatttccacc
    28981 caatagatat ttatcaaaat tataccttac cccaaggcca atattcccga tcgtacgatc
    29041 ttcttttcgt tgcgcagaaa attggctgaa ataaacagtg gtttgattat cgtaccaggg
    29101 aacaaaataa tcgatggaac tgccatccag atcgccgccc tgaccaatgg agaggttcaa
    29161 acggacctta ccgtagggag agagtgtgtc ttcaatataa gattgtgcgg ttgaggtgat
    29221 ttgactttgc agatagcttc tggcctgaga ttccagcgcc tcaggggtca agttttcaaa
    29281 cccttgcgtg gcgaagttga tgacgtaatc ggctccgcgc tctttcagcg atttaccctt
    29341 gttggtttgt tcatcctgtt gtgctgcctg gctacctaaa tcaggtaacg attcgttttg
    29401 cttaacattt atctctggcg ccgcgaaagc agcaccagag ataaatgtta gaataataca
    29461 gcgtttccat cgcagcatgc actttccctg tgacaatgat acagtcaaaa ataagcttta
    29521 atattgtatc cctgattatt cagaaagg
//
LOCUS       DAZFRE010000063        29526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFRE010000063 DAZFRE010000000
VERSION     DAZFRE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.063"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(227..772)
                     /gene="orn"
                     /locus_tag="ACHKUI_003105"
     CDS             complement(227..772)
                     /gene="orn"
                     /locus_tag="ACHKUI_003105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6599259.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            867..1919
                     /gene="rsgA"
                     /locus_tag="ACHKUI_003106"
     CDS             867..1919
                     /gene="rsgA"
                     /locus_tag="ACHKUI_003106"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6599260.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2016..2984
                     /gene="asd"
                     /locus_tag="ACHKUI_003107"
     CDS             2016..2984
                     /gene="asd"
                     /locus_tag="ACHKUI_003107"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6599261.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3006..6329
                     /gene="mscM"
                     /locus_tag="ACHKUI_003108"
     CDS             3006..6329
                     /gene="mscM"
                     /locus_tag="ACHKUI_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6599262.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6360..6674)
                     /gene="yjeO"
                     /locus_tag="ACHKUI_003109"
     CDS             complement(6360..6674)
                     /gene="yjeO"
                     /locus_tag="ACHKUI_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO6599263.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6772..8274)
                     /gene="yjeM"
                     /locus_tag="ACHKUI_003110"
     CDS             complement(6772..8274)
                     /gene="yjeM"
                     /locus_tag="ACHKUI_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6599264.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(8493..9470)
                     /gene="epmA"
                     /locus_tag="ACHKUI_003111"
     CDS             complement(8493..9470)
                     /gene="epmA"
                     /locus_tag="ACHKUI_003111"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6599265.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLLEVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9795..11603
                     /gene="frdA"
                     /locus_tag="ACHKUI_003112"
     CDS             9795..11603
                     /gene="frdA"
                     /locus_tag="ACHKUI_003112"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6599266.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11596..12330
                     /gene="frdB"
                     /locus_tag="ACHKUI_003113"
     CDS             11596..12330
                     /gene="frdB"
                     /locus_tag="ACHKUI_003113"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6599267.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12341..12736
                     /gene="frdC"
                     /locus_tag="ACHKUI_003114"
     CDS             12341..12736
                     /gene="frdC"
                     /locus_tag="ACHKUI_003114"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6599268.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12747..13106
                     /gene="frdD"
                     /locus_tag="ACHKUI_003115"
     CDS             12747..13106
                     /gene="frdD"
                     /locus_tag="ACHKUI_003115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6599269.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13169..14302
                     /gene="ampC"
                     /locus_tag="ACHKUI_003116"
     CDS             13169..14302
                     /gene="ampC"
                     /locus_tag="ACHKUI_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HFO6599270.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14391..14924
                     /gene="blc"
                     /locus_tag="ACHKUI_003117"
     CDS             14391..14924
                     /gene="blc"
                     /locus_tag="ACHKUI_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6599271.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14921..15238)
                     /gene="sugE"
                     /locus_tag="ACHKUI_003118"
     CDS             complement(14921..15238)
                     /gene="sugE"
                     /locus_tag="ACHKUI_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6599272.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15414..15560)
                     /gene="ecnB"
                     /locus_tag="ACHKUI_003119"
     CDS             complement(15414..15560)
                     /gene="ecnB"
                     /locus_tag="ACHKUI_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6599273.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGIGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15671..15796)
                     /gene="ecnA"
                     /locus_tag="ACHKUI_003120"
     CDS             complement(15671..15796)
                     /gene="ecnA"
                     /locus_tag="ACHKUI_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6599274.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15848..16414)
                     /gene="efp"
                     /locus_tag="ACHKUI_003121"
     CDS             complement(15848..16414)
                     /gene="efp"
                     /locus_tag="ACHKUI_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6599275.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16456..17484
                     /gene="epmB"
                     /locus_tag="ACHKUI_003122"
     CDS             16456..17484
                     /gene="epmB"
                     /locus_tag="ACHKUI_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6599276.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17719..18588
                     /gene="yjeJ"
                     /locus_tag="ACHKUI_003123"
     CDS             17719..18588
                     /gene="yjeJ"
                     /locus_tag="ACHKUI_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO6599277.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18638..18991)
                     /gene="yjeI"
                     /locus_tag="ACHKUI_003124"
     CDS             complement(18638..18991)
                     /gene="yjeI"
                     /locus_tag="ACHKUI_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6599278.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19129..20775)
                     /gene="groL"
                     /locus_tag="ACHKUI_003125"
     CDS             complement(19129..20775)
                     /gene="groL"
                     /locus_tag="ACHKUI_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6599279.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20819..21112)
                     /gene="groS"
                     /locus_tag="ACHKUI_003126"
     CDS             complement(20819..21112)
                     /gene="groS"
                     /locus_tag="ACHKUI_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6599280.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21388..22644
                     /gene="yjeH"
                     /locus_tag="ACHKUI_003127"
     CDS             21388..22644
                     /gene="yjeH"
                     /locus_tag="ACHKUI_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6599281.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAELWLFLPKRKTPENGITT"
     gene            complement(22660..23136)
                     /gene="fxsA"
                     /locus_tag="ACHKUI_003128"
     CDS             complement(22660..23136)
                     /gene="fxsA"
                     /locus_tag="ACHKUI_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6599282.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23473..24909
                     /gene="aspA"
                     /locus_tag="ACHKUI_003129"
     CDS             23473..24909
                     /gene="aspA"
                     /locus_tag="ACHKUI_003129"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6599283.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            25027..26328
                     /gene="dcuA"
                     /locus_tag="ACHKUI_003130"
     CDS             25027..26328
                     /gene="dcuA"
                     /locus_tag="ACHKUI_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6599284.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26444..26782
                     /gene="cutA"
                     /locus_tag="ACHKUI_003131"
     CDS             26444..26782
                     /gene="cutA"
                     /locus_tag="ACHKUI_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6599285.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26758..28455
                     /gene="dsbD"
                     /locus_tag="ACHKUI_003132"
     CDS             26758..28455
                     /gene="dsbD"
                     /locus_tag="ACHKUI_003132"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6599286.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGSGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTVQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28492..29067
                     /gene="yjdC"
                     /locus_tag="ACHKUI_003133"
     CDS             28492..29067
                     /gene="yjdC"
                     /locus_tag="ACHKUI_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6599287.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            29174..29249
                     /locus_tag="ACHKUI_003134"
     tRNA            29174..29249
                     /locus_tag="ACHKUI_003134"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29207..29209,aa:Phe,seq:gaa)
     gene            29447..>29526
                     /locus_tag="ACHKUI_003135"
     CDS             29447..>29526
                     /locus_tag="ACHKUI_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO6599288.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGM"
BASE COUNT         7283 a         7706 c         7439 g         7098 t
ORIGIN      
        1 actgagctat tcccgcgtac tacttaattt tgctttcgta attttcgcat ttctgcgtcg
       61 ttacgggagg cgcattatac gagaaatctt tccctctgca acccccctaa aaaatttttt
      121 ttgagttttt atctcaagtg ctgaaattat cagcaagacg ttcaatttag cgtcaaaatc
      181 acgccgccgc aagtggattc cggcgcttac gtgaccagga aaaattttac agcttgataa
      241 aatgctcgcg gtagtaagcc aactccgcca ccgattcacg gatatcatcc atcgcctgat
      301 gcgtcccctg cttggtaaag ccgtcaagaa tttccggctt ccagcggcgc gccagctctt
      361 tcagggtgct aacatcgaga tagcggtagt ggaagtaggc ttccagctcc ggcatgtatt
      421 taaacaggaa gcgacggtcc tgaccgatgc tgttaccaca aatcggcgat tttcccgcag
      481 gcacccactg ttttaagaat tcgagcgttg ccagttcagc ttcacgatcg cccatcgtgc
      541 tcgctttcac acgctctacc agcccgctgg cggtatgggt gcgcacgttc cagtcatcca
      601 tcagcgccag ctgttcatcg gactgatgta ctgcaatggt cggcccttct gccagaatat
      661 tcaggttggc atcggtcacc agcgtggcaa tctcaataat gcgatcgcgc tcgggatcca
      721 gaccggtcat ctcaagatcg atccaaatca ggttgttttc attggcactc atgctatttt
      781 ccacccattc acagtgacta tattgatcca caattgcgtg tatcatagat gttttgccca
      841 tcaggggcga ccaggagtct gtacgattga gtaaaaataa actctccaaa ggccagcagc
      901 gccgcgtgaa cgccaatcac cagcgtcgtc ttaaaacgtc taaggagaag cccgactacg
      961 acgacaatct gtttggcgag cctgatgaag gtatcgtcat cagccgcttt ggtatgcacg
     1021 ctgatgtgga atccgccgat ggcgacgttc accgctgcaa tattcgccgt accatccgtt
     1081 cgttggtgac cggcgaccgc gtagtctggc gtccgggtaa accagcggcg gaaggcgtaa
     1141 atgtcaaagg gatcgtcgaa gcggtgcatg agcgcacctc agtgttgacg cgcccggatt
     1201 tttacgacgg tgtgaaacct attgctgcca acatcgacca gattgtcatt gtctccgcca
     1261 ttttgccgga gctgtcgctc aatattatcg accgttacct ggtggcctgc gaaaccttgc
     1321 agattgagcc gattattgtg ctcaacaaga tagacctgct ggacgacgaa ggcatggagt
     1381 tcgtcaacga gcagatggat atctaccgca atatcggtta tcgcgtattg atggtttcca
     1441 gtcatactca ggatgggctg aaaccgctgg aagaggcgtt gaccgggcgc atcagcattt
     1501 ttgccgggca gtctggcgtt ggcaaatcca gcctgctgaa cgcgttgctg gggctgcaaa
     1561 aagagatcct gaccaacgat gtctctgaca actcggggct tggtcagcac accacgaccg
     1621 ccgcacgact gtatcacttc ccgcacggcg gtgatgtgat tgattcccca ggtgtgcgtg
     1681 agtttggtct ctggcacctg gagccggaac aaatcactca gggctttgtc gaattccatg
     1741 actatttagg tctgtgtaaa tatcgcgatt gcaaacacga taccgatccg ggctgcgcta
     1801 tccgggaagc ggttgaggaa gggaaaatcg cggaaacccg tttcgaaaac tatcaccgta
     1861 ttctggaaag catggcgcag gtaaaaacgc gtaaaaactt ttctgatacg gatgactgac
     1921 aattaagcta accgtcgtta gaatcgtccc ctttttttca ggatccggca tgtaggccgg
     1981 atcaggaacg acaaaacaat ggcctggagg ctaccttgtt aaattcattt aaactttcgc
     2041 tacagtacat tctgccgaaa ctatggctta ctcgcctggc gggttggggc gcaagcaagc
     2101 gggcaggatg gctgacaaaa ctggttatcg atctgttcgt taaatactac aaggtcgaca
     2161 tgaaagaggc gcaaaagccg gacaccgcca gctaccgcac ctttaacgaa ttctttgtcc
     2221 gtccgctgcg tgacgaagta cgcccaatcg ataccgatcc gaatgtactg gttatgcctg
     2281 ccgatggcgt tatcagccag ttgggtaaaa tcgaagaaga taaaatcctg caagccaaag
     2341 gccacaacta cagcctcgaa gccctgctgg caggcaacta tctgatggcg gatctcttcc
     2401 gtaacggtac gtttgtgacc acttacctct ccccacgtga ctaccaccgc gtacacatgc
     2461 cgtgcaacgg tattctgcgt gaaatgatct acgtgccggg cgatctcttc tccgttaacc
     2521 atctcacggc tcagaacgtg ccgaatctgt ttgcccgtaa cgaacgcgtg atctgcctgt
     2581 tcgataccga atttggcccg atggcgcaga ttctggtcgg tgcgacgatt gtaggcagca
     2641 ttgagacggt ctgggcgggc accattacgc cgccgcgcga aggcatcatc aagcgctgga
     2701 cctggcctgc cggggaaaac gacggttctg tggcattgct gaaaggccag gaaatgggtc
     2761 gctttaaact tggctctacc gttatcaacc tgtttgcacc aggtaaagtg aacctggttg
     2821 agcaactgga aagcctgtct gtcacgaaaa ttggccagcc gctggcagta tctaccgaaa
     2881 cctttgttac gccagacgct gaaccagccc cgcttcctgc tgaagaaatc gaggcagaac
     2941 acgacgccag cccattggtt gacgacaaaa aagaccaggt ctaatccatc aaaggaaacg
     3001 ctgacgtgcg cctgattatc acttttctga tggcctggtg cctcagttgg ggggcgtacg
     3061 ccgcgacggc ccccgatagc aaacaaatca cccaggaact ggagcaggca aaagcggcga
     3121 aacctgcgca gccggaagtc gtagaggcgc tccagtctgc cttaaatgcg cttgaggaac
     3181 gaaaaggttc ccttgagcgc atcaagcaat atcaagaagt cattgataat tatccgaaac
     3241 tttccgccac tctgcgcgcg cagttaaaca acatgcgtga cgagccacgc agcgtgtcgc
     3301 cgggaatgtc taacgatgcg ctgaatcagg aaattctcca gatcagcagt cagttgctgg
     3361 ataaaagccg tcaggcccag caagagcagg agcgcgcccg cgagattgcc gattcgctga
     3421 atcaactgcc gcaacagcaa accgatgccc gccgtcagtt gaatgaaatc gagcgccgcc
     3481 tggggacact taccggcaat accccgctca atcaggcaca aaatttcgcg ttgcagtctg
     3541 actccgcacg tctgaaagcg ctcgttgatg aactggagct ggcgcagcta tctgccaata
     3601 accgccagga attagcgcgc ttgcgctctg agctggcgga aaaagagagc cagcagctgg
     3661 atgcatattt gcaagcacta cgcaaccagt tgaatagtca acgccaactg gaagcggaac
     3721 gggcgctgga aagtaccgag caactggcgg aaagtagcgc cgatttgcca aaagatatcg
     3781 tcgcccagtt taaaattaac cgcgaactat cggcggcgct gaatcaacag gcgcagcgga
     3841 tggatctcgt tgcctcgcaa cagcgtcagg ctgccagcca gacgttacag gtccggcagg
     3901 cgttgaatac gctgcgtgaa cagtcgcaat ggctgggatc atctaacctg ctcggcgaag
     3961 cgttgcgggc gcaggtggca cggctaccgg aaatgccaaa accacaacag cttgataccg
     4021 aaatggcgca gttacgtgtg caacggttac gttatgagga tctgcttaat aaacagccgc
     4081 tgctacggca aatccatcag gccgacggtc agccactgac cgctgagcaa aaccgtattc
     4141 tggaagcaca gctacgcact cagcgtgagt tgctgaactc attgttgcag ggtggcgaca
     4201 cgctactgct ggagctgacc aagctgaaag tctccaacgg gcaactggag gatgcgctga
     4261 aagaggtgaa cgaagcgacg caccgctatc tgttctggac ctctgacgtg cgcccgatga
     4321 ccatcgcctg gccgctggaa atcgcccagg atctgcgtcg tctcatttcg ctggacacct
     4381 tcagtcagtt gggcaaagcc agtgtgatga tgctgaccag taaagagacg attttgccgc
     4441 tgtttggcgc gttgattctg gtcggttgca gtatttactc gcgccgctat ttcacccgtt
     4501 ttcttgaacg ttcggcggcg aaagtcggca aagtgactca ggatcacttc tggctgacgt
     4561 tacgcactct tttctggtcg attctcgtcg cgtcaccgct gcctgtgctg tggatgacac
     4621 tgggttacgg cttgcgcgag gcgtggcctt atccgctggc ggtcgcgatt ggtgatggcg
     4681 taacagccac cgtgccgctg ctgtgggtag tgatgatttg cgccaccttt gctcgcccga
     4741 acggcttgtt tatcgctcat tttggctggc cgcgcgaacg agtttcccgt gggatgcgtt
     4801 actacctgat gagcatcggg cttattgtgc cgctgattat ggcgctgatg atgttcgata
     4861 acctcgacga ccgtgaattc tccggttcgc tgggacggct ttgctttatc ctcatttgcg
     4921 gtgcgctggc ggtggtcacc ctcagcctga aaaaggccgg gatcccgctg tatctcaaca
     4981 aagagggcag cggcgacaac attaccaacc atatgctgtg gaacatgatg attggcgcac
     5041 cgctggttgc cattctggcg tcggcggtgg gttatctggc aacggcgcag gcgctgttag
     5101 cgaggcttga aacctcggtt gccatctggt ttttgctact ggtggtttat cacgttatcc
     5161 gccgctggat gctgatccag cgccgcaggc tggcgtttga ccgggcgaag catcgccggg
     5221 cagagatgtt agcgcagcgc gcgcgtggcg aagaggaagc gcatcatcac agtagcccgg
     5281 aaggagcaat tgaagtcgat gaaagcgaag tcgatctcga tgccatcagt gcgcaatcct
     5341 tgcggctggt gcgctcaatt ttgatgttga tcgccctgct ttcggtcatt gtgctgtggt
     5401 cagaaatcca ttctgctttc ggcttcctcg aaaatatttc gctgtgggat gtcacttcca
     5461 cggtacaggg cgtagaaagc ctggagccga ttaccctcgg tgcggtactg attgccattc
     5521 tggtgtttat catcaccacg cagctggtgc gcaacctgcc cgcgctgctg gaactggcga
     5581 ttctgcagca cctggattta acgccgggta cgggctacgc catcaccacc atcaccaaat
     5641 atctgctgat gctgattggc gggctggtcg gcttctcgat gattggtatt gagtggtcga
     5701 aattgcagtg gctggtcgcc gcgctcggtg ttggtctggg ctttggttta caggagattt
     5761 tcgccaactt tatctctggc ctgattatcc tgttcgaaaa accgattcgt attggcgata
     5821 cggtaacgat ccgcgatctc accggtagcg tgacaaaaat taacacccgc gccaccacca
     5881 tcagcgactg ggaccgtaaa gagataattg tgccgaacaa ggcgtttatt accgagcagt
     5941 ttatcaactg gtcgctctct gactcggtca cgcgcgtggt attgacgatt ccggcccctg
     6001 ccgatgccaa cagtgaagaa gtgacggaaa tcctgctcac cgcagcgcgt cgctgctcgc
     6061 tggtgatcga caacccggcg ccggaagtct tcctggtgga tctgcaacag gggattcaaa
     6121 ttttcgagct gcgtatttac gccgctgaga tgggtcaccg tatgccacta cgccatgaga
     6181 ttcaccagct aattctggca ggattccatg cccacggtat tgatatgcca ttcccgccct
     6241 tccagatgcg tttggaaagc ctgaatggta agcaaacggg gagaacatta acttctgcgg
     6301 gcaaaggtcg tcaggcggga agtttgtaag gtctttgtgg ccggatgaac ccatccggct
     6361 tataagcaaa actgatagcg cagaaaaaac tgccacagcc aatagccgaa aatagccagc
     6421 gcggtggcgt acataaacca gccgcgtttt tttcgccaca aagcgaacca gagaaaaggg
     6481 aaaaagagcg gtaacgtcat tatcacaccg acatcgcgag tatcgtcatc ttcgagatac
     6541 atcaaaacat cacagacgtt gttaatcccg cgtccatcaa tcatccactc tttgtccagc
     6601 gcggaggtaa tggttatcca gaggaacgcc acaatgaacc agatgcagca caagacagac
     6661 atgcgcgcac tcatgaggta acccatagtt taatttcgct atagcgcata cgaaatctca
     6721 ctaattccct gtcattacgc cctatcgtca ggccgacgat agggcatttg attactcagc
     6781 cacccactgc ggatttttta ccactctgct gcaatagttc tggtaaatcg ccatcgctaa
     6841 cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc
     6901 aaccacaggt tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac
     6961 tgttgcaatc attgccgaca tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg
     7021 acgtgcttta aaaaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac
     7081 gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac
     7141 cagcaggatg aagacagtaa ccaacccaca ctgcatccac atggcgatag acggcatccc
     7201 catcgcattc aggcgcgtca tcggttccgg ccacaatgct ttcggcgtcc cctggatgat
     7261 ggctttcagc ggtgaatagc aaagtgtaaa gaacgcaccg gtataagcga ggaacatcga
     7321 cagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg
     7381 cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccgag
     7441 gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat
     7501 tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt
     7561 cttttctgga ttttccgttt tatcaaccag accaccaacc gcttcaattc cgccataggc
     7621 aaaaatggca aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg
     7681 tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat
     7741 gcttactaac agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat
     7801 gcgggcaatt ttattaatcc cttttgaagc aacgacggtg accagaatca tccatgccac
     7861 tgccagcaga ccaaccacct gcgtaggctc cagtccggca atatgccagt gctgggtcat
     7921 gtcgctaccg tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat
     7981 ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac
     8041 actattattc atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc
     8101 agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat
     8161 cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa atacagaagt
     8221 aaagatcatc aatatgagtc ctatcagact catctttttt atcgagtgag gcatgaaacc
     8281 atccttcatt tcagcgcgtg tataaaacgc gcccactgaa ctgcactgcc caacattgag
     8341 cgggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta
     8401 aattgtggcc tttagcggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca
     8461 ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg
     8521 acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc
     8581 acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg
     8641 tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc
     8701 tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc
     8761 tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg
     8821 gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag
     8881 gtaaacagca attgtagaag cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa
     8941 tccagtttcg ctgccacttc cagcagttgc gttttgtcgg cagatagtgg gtcaatttcc
     9001 agataacgca agaaagcttg ttgataagaa aggctttctg ctgccggaca gtccagcacc
     9061 tgttgcaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac
     9121 cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg
     9181 cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgcttcat atggtattcc
     9241 gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc
     9301 tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc
     9361 tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt
     9421 aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct
     9481 ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc
     9541 aggaataaaa caacggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta
     9601 aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca
     9661 aatttcagac ctatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg
     9721 ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa
     9781 tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg
     9841 cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa
     9901 tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg
     9961 tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact
    10021 ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc
    10081 aactggaact gtgggggtgc ccatggagcc gtcgtccgga tggtagcgtc aacgtacgtc
    10141 gcttcggcgg catgaaaatc gagcgtacct ggttcgccgc cgataagacc ggcttccata
    10201 tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac
    10261 atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca
    10321 tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg
    10381 cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta
    10441 tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg
    10501 gtctaccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggt ggtattctgg
    10561 tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg
    10621 gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct
    10681 ggcacgaatg gcgtaaaggc aacaccatct ccacgccacg tggcgatgtg gtttacctcg
    10741 acctgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg
    10801 cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac
    10861 actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt
    10921 tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc gaaccgtctg ggctccaact
    10981 cgctggcgga actggtggtc ttcggtcgtc tggccggtga acaagcgaca gagcgtgcag
    11041 caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac
    11101 gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggctaagatc cgcgacgaaa
    11161 tgggcatggc catggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa
    11221 ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatt accgacactt
    11281 ccagcgtgtt caacaccgac ctgctctaca ccattgagct gggccacggt ctgaacgttg
    11341 ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgcg caccagcgtc
    11401 tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc
    11461 gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc
    11521 cagctaaacg tgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata
    11581 agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac
    11641 ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc
    11701 tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc
    11761 tggtcctgcc gtatggcgat ttgtggctcc tgcggcatga tggttaacaa cgtgccaaaa
    11821 ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct
    11881 aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa
    11941 gcgatcaaac cgtacatcat cggcaacgcc cgcaccgcgg atcagggtac taacatccag
    12001 accccggcgc agatggcgaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc
    12061 tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg
    12121 ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag
    12181 ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc
    12241 ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac
    12301 tttcttatcg cgactctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc
    12361 gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat
    12421 gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcggcct
    12481 gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc
    12541 ggttatcgtg atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg
    12601 gtttgagctg gcaccaaaag cagccaatat cattgtaaaa gacgaaaaaa tgggaccaga
    12661 gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt
    12721 tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac
    12781 cggtattctg gggcctcttc ggggccggtg gtatgtggag cgccatcatt gcgccggtga
    12841 tgatcctgct ggttggtatt ctgctgccgc tagggctgtt tccgggtgat gcgctgagct
    12901 acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga
    12961 tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa
    13021 tccacgtacc tgcgggtaaa tgggttttct acggtctggc tgctatcctg acagttgtca
    13081 cactgattgg tgtcgttaca atctaacgca tcgccaatgt aaatctggcc cgcctatggc
    13141 gggccgtttt gtatggaaac cagaccctat gttcaaaacg acgctctgca ccttattaat
    13201 taccgcctct tgctccacat ttgccgcacc tcaacaaatc aacgatattg tgcatcgcac
    13261 aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtagcgg taatttatca
    13321 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
    13381 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
    13441 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc acaacaaaat actggcctga
    13501 acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct acaccgctgg
    13561 cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc tgcgcttcta
    13621 tcaaaactgg cagcctgcat gggctcctgg aacacaacgt ctgtatgcca actccagtat
    13681 cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa
    13741 aactcgtgtc ttccagccac tcaaactcaa tcatacgtgg attaatgtac cgtccgcaga
    13801 agaaaagaac tacgcctggg gatatcgcga aggtaaggca gtacatgttt cgccaggggc
    13861 gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
    13921 aagcaattta aaaccccttg atatcaatga gaaaacactt caacaaggga tacaactggc
    13981 acaatctcgc tactggcaaa ccggcgatat gtatcagggt ctgggctggg aaatgctgga
    14041 ctggccggta aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
    14101 acgccccgta aaaccgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
    14161 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
    14221 tatcgtgatg ctggctaaca aaaactaccc caatccagcg agagtcgccg ccgcctggca
    14281 gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctccgc
    14341 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
    14401 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tcccctacgc
    14461 cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgttatctc ggtacctggt
    14521 atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat
    14581 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
    14641 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
    14701 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
    14761 aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca
    14821 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
    14881 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttaatgagtg ctgagtttca
    14941 gaccaataat ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact
    15001 caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
    15061 cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc agactgacaa
    15121 tcatcgccgt cacggtaata acacttggcg tcaaacgact aaagccgtga gtatatttca
    15181 ggccaacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
    15241 caggctccgg aaaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca
    15301 aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact attacctatg
    15361 taactatcgc agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc
    15421 tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaatg
    15481 ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct
    15541 gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat
    15601 agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggagg
    15661 agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gagatgtttg atatcttcgc
    15721 cgaaaccgcg agcggtgtta cagcccgtga gcagcgcgct ggcaagcaaa acaagaacga
    15781 taaggcgttt catcatctct gacctgaaaa agtgatggtg cagcctgcag gccgcaccac
    15841 aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt
    15901 cgccgatctg tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt
    15961 tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt
    16021 caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact
    16081 ctgcctgatc cagcaaccat ttagcgttgt caccaattgc ttttgcatca gcagacagct
    16141 gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca
    16201 ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta
    16261 cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg
    16321 gttttacgaa ttcactcgct tcaactgcgt aaggttcgcc gtctaacatg attttaagac
    16381 cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg
    16441 tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg
    16501 ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata
    16561 gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg
    16621 ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag
    16681 gtacttacct cgcaagatga gtttgtcgtc gcgcccggat tctccaccga cccgctggaa
    16741 gaacagcata gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg
    16801 gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc
    16861 gaaaatcagg gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg
    16921 gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg
    16981 gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc
    17041 cgtctgccga ttgtgatccc ggcacgcatc acagatgcgc tggttgaacg cttttcacat
    17101 tctacgctgc aaatcttgct ggtgaatcac atcaaccatg ccaatgaggt agatgaaaca
    17161 ttccgtcagg cgatggctaa gttgcgccgg gtgggcgtta ctttgctgaa ccagagcgtt
    17221 ctgttacgtg gtgtgaacga taacgcgcaa acgctggcaa acctgagtaa tgcgttgttc
    17281 gatgcgggcg tgatgccgta ttacctgcat gtgctcgata aagtacaggg cgcggcgcat
    17341 tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg
    17401 ggatatctgg tgcctaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg
    17461 gatctccagc tacgccagca gtaactgatt aaacagggaa gtttaatctt tgcccgttgc
    17521 ataaccttta attattcata tacattgctg ttgttactct ttatataacc aacaccaata
    17581 attaaccttt cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac
    17641 tattactacc aagcccattt atctggctta taaactaaaa ttaattattt aattgagaaa
    17701 tggttaggga gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc
    17761 aagagtaata atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg
    17821 ttgttcttta tgtctgccat ggaattgcgc gaccttctga ttgcgctgga aagtcgtctg
    17881 catcaaaagc ataaactgga tgctgctgca cgccttcagt atgaacaagc tcgcgataaa
    17941 gtcattaaga aaatggcaga aaatatccca gaaattctgg ttgatgagct taaaaatgcc
    18001 gatattaatc gtcgggttaa cacgctggaa cttaccgatg atcagggcga aaatcttacc
    18061 ttcgtattaa cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt
    18121 gaaatgctgg cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca
    18181 ttacgtatta cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggaaaat
    18241 ggcaatctgg aatatgacac ctattcccaa ccggaatgga aacataactt gttcaaccat
    18301 tatctggcgg tgctttaccg ctttaaagat gaaagtggca atgaacaatt tagcggcgca
    18361 gtggtgaaaa cgcgtgaagc aacgccgggt aaagaagtgg aggcgatcac tcgccgaatg
    18421 cttgatttca gcccccgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc
    18481 accgtagcgg caaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat
    18541 cacttacgtg ttcaatccac cagcaaaact gcaccacagg cgaaataatc tcatctgttt
    18601 tactacgagg ctggaagtat ccagcctctg gatcacatca gttcgggcac ttataaacct
    18661 gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc
    18721 tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg
    18781 cgccgcgcat agaaccgcct tcttctccgt gttgcccgga aagccagttg ctttgcttac
    18841 ctgtcgcagt accaatcagc tggcactctg cacctggttg ctcgtccaca atgcgtacac
    18901 tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac
    18961 cgacaatccc tgctaaatat tttacgtgca tgttattccc cataatcaat gagctggacg
    19021 ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc
    19081 agacatttct gcctgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc
    19141 gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt
    19201 cggcaggtcg gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta
    19261 ctgcagagca gaacgggtta ctttggttgg atccaggata cccatatcga tcatgttgcc
    19321 gtattcttca gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc
    19381 aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc
    19441 acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag
    19501 tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc
    19561 agcacgggtc gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc
    19621 ggtagcagca ccaactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg
    19681 cagtttttca cgatcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
    19741 acggccctgg attgcagctt cttcacccac gccatcgatg atggtggtgg tgtctttgtt
    19801 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
    19861 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
    19921 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
    19981 gttaacaacc agagttgcca gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
    20041 tttacctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tggagatttt
    20101 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
    20161 gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc
    20221 gtcctgcaga ccggtaccgt cttcaacggt gataacacct tctttaccga ctttgtccat
    20281 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg
    20341 agcaatcgct ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc
    20401 ggtaacagct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
    20461 tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc
    20521 tgcagcgtcg ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
    20581 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
    20641 accgaaagat ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc
    20701 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
    20761 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt
    20821 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
    20881 tcttctcaga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat
    20941 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
    21001 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
    21061 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
    21121 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
    21181 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
    21241 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct
    21301 tcatcaccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccat
    21361 aatttgggcg caatcggaca tcaacccatg agtggactca aacaagaact ggggctggcc
    21421 cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
    21481 ttagctgcgc tagtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta
    21541 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc
    21601 gtcgcacact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggttg
    21661 tttttatcgg tcattcccgt gggtttgcct gccgcgctac aaattgctgc cggattcggc
    21721 caggcaatgt ttggctggca tagcgggcaa ctgttgttgg cagaactcgg tacgctggcg
    21781 ctggtgtggt atatcggtac tcgaggtgcc agttccagtg ctaatctaca aacagttatt
    21841 gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat caaacctgcg
    21901 aatatcccct tccctgcgcc aggaaatatc gaacttaccg ggttatttgc tgcgttatca
    21961 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
    22021 aatccagagc gtgattttcc tcgtgctttg atgattggcc tgctgctggc aggattagtc
    22081 tattggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggcg
    22141 gcagcatcgc ttcccaaaat tgtagtgcag ttgttcggtg taggagcgtt atggattgcc
    22201 tgcgtaattg gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc
    22261 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
    22321 cgccatattc cgaataatgc cctcaatgcg gtgctcggct gctgcgtggt gagcacgttg
    22381 gtgattcatg ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
    22441 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga
    22501 ctactggcag tggttggcgg gctattatgc gttctgttac tggcaatggt cggctggaaa
    22561 agtctctacg cgctgatcat gctggcggag ttatggctgt ttctgccaaa acgaaaaacg
    22621 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
    22681 atgatcaagg cggtcgcggt catcatcctt tcgctggtac tcaccatcaa aagtattacc
    22741 gccaccggtc ccggcgctaa aaccgccacc tggcatacgg gaaaaacgca gatgaggcat
    22801 caacttcact gtcagatgct tttgcaccgg cggtaataaa agaagcaaac cgaggaaatc
    22861 ggtgaaaaag cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat
    22921 catctccgcc gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
    22981 aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag
    23041 ggtcagcaat acccccaata catgagcaac ctgaataaag attgaaatct caatatagac
    23101 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
    23161 gttaaacccg gctacctgaa tgggttgcga atcgcgttta gcttatattg tggtcattag
    23221 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatccatt
    23281 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccagg ttacggtaga
    23341 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
    23401 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
    23461 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
    23521 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
    23581 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
    23641 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
    23701 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
    23761 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
    23821 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
    23881 catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt
    23941 tactcttccc tgattaaact ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
    24001 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
    24061 ccgatgaccc tcggtcagga attccgcgcc ttcagcatcc tgctgaaaga agaagtgaaa
    24121 aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
    24181 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
    24241 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctccga ctgcggcgct
    24301 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
    24361 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
    24421 ctgcaggcag gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
    24481 aaccaggtct gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
    24541 ggtcagctgc agttgaacgt tatggagcca gtcattggcc aggccatgtt cgaatccgtt
    24601 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
    24661 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
    24721 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
    24781 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
    24841 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
    24901 gaacagtaat cgtacagggt agtacaaata aagaaggcac gtcagatggc gtgccttttt
    24961 tcttgtgagc agtaacttaa aaataacaat ttaatatcaa cttgttaaaa aacaaggaag
    25021 gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga
    25081 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
    25141 ggcgttaaac ccggtaacat cccattcgat gtcatttcca ttatcatggc ggttatcgcc
    25201 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
    25261 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
    25321 actatctttg ctggtactgg caacatctct ctggcgacac taccagttat cgctgaagtt
    25381 gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag
    25441 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
    25501 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
    25561 gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg
    25621 atttatcgca agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa
    25681 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
    25741 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccact gatgaacacc
    25801 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
    25861 gtcgataccg acaacattct caactccagc accttcaaag caggtatgag cgcctgtatt
    25921 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
    25981 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
    26041 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct
    26101 ctggcactga acgtttctcc gctgaccgct gttgcttctt ttgctgcggt gtctggtctg
    26161 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact
    26221 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
    26281 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg acccatcgcg
    26341 gggcgttcac gccccgcttt ctttcccgcc gactaacatc ctttccccgt ccgttgtata
    26401 gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
    26461 ttcgaatacc acgtctgtcg tggtgctatg tacggcaccg gatgaagcga cagcccagga
    26521 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
    26581 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
    26641 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
    26701 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
    26761 gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta
    26821 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc ttttgatttt
    26881 cagcaaaacc aacatgacct gaatctgacc tggcagatca aagacggtta ctacctctac
    26941 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa
    27001 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
    27061 cttcccgtaa ccatcaacca ggcgagtgcg ggagcaacgt taactgtcac ctaccagggc
    27121 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
    27181 gtcgccaaca acgaagcgtc acagcctgtg tctgttccgc agcaagagca gcccaccgcg
    27241 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
    27301 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggcggtaa acagcggctt
    27361 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc gctgacttac
    27421 acggcgctgg gtctggtggt tgccgccgca gggttacagt tccaggcggc gctacagcac
    27481 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
    27541 ttgtttactc tgcaactccc ctcttcgctg caaacacgtc tcacgctgat gagcaatcgc
    27601 caacagggcg gctcacctgg cggtgtgttt attatggggg cgattgccgg actgatctgt
    27661 tcaccatgca ccactgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
    27721 atgtggctgg gcagcggcac gctttatctt tatgcgctgg gcatgggcct gccgctgatg
    27781 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
    27841 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
    27901 ggtgatatat ggggattacg cttgtggtcg gcgcttggtg tcgcattctt tggctgggcc
    27961 tttatcacca gcctacaggc caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
    28021 gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc
    28081 gtgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
    28141 gcgctcgttg aagccaaagg caaaccagtg atgttagatc tctatgccga ctggtgcgtc
    28201 gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca
    28261 gacacggtct tacttcaggc taacgtcact gccaacgacg cacaagatgt ggcgctgtta
    28321 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
    28381 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
    28441 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
    28501 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
    28561 ctggagatgg ttgctgaacg tgtggattac ccactggacg agctacgccg cttctggcca
    28621 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
    28681 cgtcgtcagc tgatgctgga cgaaacacaa accgccgaac aaaagttgct ggcacgttat
    28741 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctgtt tatcgctgcc
    28801 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
    28861 gcggcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
    28921 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
    28981 agccaggcgg atgtcgacac cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
    29041 tgccgccagg gtggtgcttt gacctgacag aaacacagaa aagaagcgat ttgccgcaat
    29101 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt
    29161 aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt
    29221 ccttggttcg attccgagtc cgggcaccaa attcatatca acggacctcc acggaggtcc
    29281 gtttttcgtt tcagaacgcc acaatttaag cgttctgcct ccaaatcaat tctaccgaac
    29341 tcaaccagat tccccccaca tcaaccccat tgtgtgggta taattgcggg tataccccag
    29401 ttcgacagaa tttgtaccct ctttaacgca ttcagaaggc tgaactatgg cactgactga
    29461 cgcaaaaatc cgggctgcaa agcacactga caaggcttat aaactcactg acggggctgg
    29521 catgtt
//
LOCUS       DAZFRE010000064        29312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFRE010000064 DAZFRE010000000
VERSION     DAZFRE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.064"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..351
                     /locus_tag="ACHKUI_003136"
     CDS             <1..351
                     /locus_tag="ACHKUI_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6599289.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            507..2042
                     /gene="gadC"
                     /locus_tag="ACHKUI_003137"
     CDS             507..2042
                     /gene="gadC"
                     /locus_tag="ACHKUI_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6599290.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2173..3492
                     /gene="digH"
                     /locus_tag="ACHKUI_003138"
     CDS             2173..3492
                     /gene="digH"
                     /locus_tag="ACHKUI_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6599291.1"
                     /translation="MDICSRNEKLAIRRPAILGALALLLCSCKSTPPESMVTPPAGSK
                     PPAMTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3870..4034
                     /locus_tag="ACHKUI_003139"
                     /pseudo
     CDS             3870..4034
                     /locus_tag="ACHKUI_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4059..6458
                     /gene="dosP"
                     /locus_tag="ACHKUI_003140"
                     /gene_synonym="pdeO"
     CDS             4059..6458
                     /gene="dosP"
                     /locus_tag="ACHKUI_003140"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO6599292.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKVW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIECVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRIFALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(6648..7079)
                     /gene="osmC"
                     /locus_tag="ACHKUI_003141"
     CDS             complement(6648..7079)
                     /gene="osmC"
                     /locus_tag="ACHKUI_003141"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6599293.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            7424..7639
                     /gene="bdm"
                     /locus_tag="ACHKUI_003142"
     CDS             7424..7639
                     /gene="bdm"
                     /locus_tag="ACHKUI_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6599294.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7741..7878
                     /gene="sra"
                     /locus_tag="ACHKUI_003143"
     CDS             7741..7878
                     /gene="sra"
                     /locus_tag="ACHKUI_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6599295.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8035..9732
                     /gene="maeA"
                     /locus_tag="ACHKUI_003144"
     CDS             8035..9732
                     /gene="maeA"
                     /locus_tag="ACHKUI_003144"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6599296.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASEMLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            9866..10876
                     /gene="adhP"
                     /locus_tag="ACHKUI_003145"
     CDS             9866..10876
                     /gene="adhP"
                     /locus_tag="ACHKUI_003145"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6599297.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            11062..11340
                     /locus_tag="ACHKUI_003146"
     CDS             11062..11340
                     /locus_tag="ACHKUI_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6599298.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            11340..11624
                     /gene="yddM"
                     /locus_tag="ACHKUI_003147"
     CDS             11340..11624
                     /gene="yddM"
                     /locus_tag="ACHKUI_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6599299.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(11853..12506)
                     /gene="fdnI"
                     /locus_tag="ACHKUI_003148"
     CDS             complement(11853..12506)
                     /gene="fdnI"
                     /locus_tag="ACHKUI_003148"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6599300.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(12499..13383)
                     /gene="fdnH"
                     /locus_tag="ACHKUI_003149"
     CDS             complement(12499..13383)
                     /gene="fdnH"
                     /locus_tag="ACHKUI_003149"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6599301.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(13396..16443)
                     /gene="fdnG"
                     /locus_tag="ACHKUI_003150"
     CDS             complement(13396..16443)
                     /gene="fdnG"
                     /locus_tag="ACHKUI_003150"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15856..15858),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6599302.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            16675..17556
                     /gene="yddG"
                     /locus_tag="ACHKUI_003151"
     CDS             16675..17556
                     /gene="yddG"
                     /locus_tag="ACHKUI_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6599303.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            17829..19217
                     /gene="narU"
                     /locus_tag="ACHKUI_003152"
     CDS             17829..19217
                     /gene="narU"
                     /locus_tag="ACHKUI_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6599304.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            19312..23052
                     /gene="narZ"
                     /locus_tag="ACHKUI_003153"
     CDS             19312..23052
                     /gene="narZ"
                     /locus_tag="ACHKUI_003153"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6599305.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMISYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDTVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADNAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCIGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            23049..24593
                     /gene="narH"
                     /locus_tag="ACHKUI_003154"
     CDS             23049..24593
                     /gene="narH"
                     /locus_tag="ACHKUI_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6599306.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            24593..25288
                     /gene="narW"
                     /locus_tag="ACHKUI_003155"
     CDS             24593..25288
                     /gene="narW"
                     /locus_tag="ACHKUI_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6599307.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPILTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            25285..25965
                     /gene="narI"
                     /locus_tag="ACHKUI_003156"
     CDS             25285..25965
                     /gene="narI"
                     /locus_tag="ACHKUI_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6599308.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRVTSSGADILILAILLIQCILGLTPIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            26045..26938
                     /gene="yddE"
                     /locus_tag="ACHKUI_003157"
     CDS             26045..26938
                     /gene="yddE"
                     /locus_tag="ACHKUI_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6599309.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDNILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(27034..27879)
                     /gene="nhoA"
                     /locus_tag="ACHKUI_003158"
     CDS             complement(27034..27879)
                     /gene="nhoA"
                     /locus_tag="ACHKUI_003158"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO6599310.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            28054..28623
                     /gene="yddH"
                     /locus_tag="ACHKUI_003159"
     CDS             28054..28623
                     /gene="yddH"
                     /locus_tag="ACHKUI_003159"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6599311.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(28940..29155)
                     /locus_tag="ACHKUI_003160"
     CDS             complement(28940..29155)
                     /locus_tag="ACHKUI_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6599312.1"
                     /translation="MFIRPVKPSDVEPLMDMLLDRDQFDQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCASEMMTNDV"
     gene            complement(29236..29312)
                     /locus_tag="ACHKUI_003161"
                     /pseudo
     CDS             complement(29236..29312)
                     /locus_tag="ACHKUI_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7128 a         7516 c         7385 g         7283 t
ORIGIN      
        1 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg tacgggtcgc
       61 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac
      121 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
      181 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa
      241 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
      301 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacgttta
      361 acggtaacgg tgtcccgaaa cgaagccgtt tcgggacaat ttccaaagtc tgttcactgg
      421 cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga
      481 caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc
      541 agctcacatt actcggattt tttgccataa cggcatcgat ggtaatggct gtttatgaat
      601 accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt
      661 tatggtttat tcccgtggga ctttgtgctg cggaaatggc taccgtcgac ggctgggaag
      721 aaggtggcgt cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga
      781 tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag
      841 gggcactctc ctacatcctg aaatggccag ctctgaatga agaccccatt accaaaacta
      901 ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca
      961 cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga
     1021 tcgcattagc ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggatgcga
     1081 agaccttctt ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt
     1141 tgagttatat gggcgttgaa gcttccgcaa cccacgtcaa tgaaatgagc aaccccgggc
     1201 gcgactatcc actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg
     1261 gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa
     1321 tgcaaacctt taccgttctg atgtcacatg tggcaccgga aattgagtgg acggttcgcg
     1381 tgatctccgc actgctgttg ctgggtgttc tggcggaaat cgcttcctgg attgttggtc
     1441 cttctcgcgg gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa
     1501 tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctatcg
     1561 cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg
     1621 cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt
     1681 tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga
     1741 aactggtcgt ggctattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc
     1801 tgccgccgga taacatccag ggtgactcta ccgatatgta tgttgaatta ctggtcgtta
     1861 gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaagca
     1921 aagcgaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact
     1981 tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact
     2041 aaccatgtct gaatggttat aagacaaggg agcgataatt catcgctccc tttttcgtgc
     2101 ttgttgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg
     2161 aaaccagcac atatggatat ctgctcccga aacgagaaat tagcgattag aagaccagcg
     2221 atactgggtg cactggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg
     2281 gtgacaccac cagcgggttc aaagccacca gccatgacgc aacaatcgtc acaaccgatg
     2341 cgtggcatct ggctggccac ggtgtctcgc ctcgactggc caccggtttc ctcggttaac
     2401 attagtaacc ccaccagccg ggcacgtgta caacaacagg cgatgatcga caaactggat
     2461 catctgcaac gtctcggcat aaacacggtc tttttccagg tcaagccgga tggtaccgcc
     2521 ctgtggccat cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat
     2581 ccgggttacg atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta
     2641 cacgcctggt ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa
     2701 ctgaatagca ccctgtctca acaaccggcc agcgtctatg tgcaacaccg cgactggatc
     2761 agaacgtccg gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc
     2821 acatcaatag tcgcggaagt ggtttcccgc tatccagtag atggcgtgca gtttgatgac
     2881 tatttctata ctgaatcacc aggttcacgg ctaaatgata acgaaacgta ccgcaaatac
     2941 ggcggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgcg
     3001 aaggtatcgc acaccattaa aagcattaag ccggaagtcg aatttggcgt tagcccagca
     3061 ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc
     3121 tatgacgaat cctacgctga tacccgtcga tgggtggaac aagggttgct ggattacatt
     3181 gctccccaaa tttactggcc attctcacgg agtgccgcgc gttatgacgt gttggcaaaa
     3241 tggtgggcgg atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat
     3301 aaagtgggtg aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa
     3361 ctgaaaaagc agctcgatct taacgatgct gtgccggaaa ttagcggcac aatcttgttc
     3421 cgtgaggact atctgaataa accgcagact caacaagcgg tcagctatct gcaaagtcgt
     3481 tggggcagtt aaactcccat tcttactttg taacaagccg gtgctttacc cactggcttg
     3541 ttaccctctt gaactatccc actagcaatc atgcttaaat acgtcaaatt aaccacgcaa
     3601 ttaaccacac ttagttaaac agccataatt cccctaataa aaatgtatga atatgaaaaa
     3661 acatacgata ttcgctgtca ccgaaaaata attacgatga gctatactct tctgaagaac
     3721 agtagcgtag attgaaactg tcctaagtcg cttatttctt ttcagtatat cttcatattt
     3781 caggagaata tggtgcagtt agtcgatctc gcacgttgtg tttggttgat tttggcctgc
     3841 ggtttaacca taaaggtcga cattatttta aagccgcaaa cggcgaggat attgccctct
     3901 cactttccat cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa
     3961 tagccgatga agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgta
     4021 aagccagtct ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggata
     4081 atgccgccga tggcattttt ttccccgccc ttgagcaaaa tatgatgggc gcggtgttaa
     4141 ttaacgaaaa tgatgaagtg atgtttttca accccgccgc agagaagctc tgggggtaca
     4201 aacgtgaaga agtcattggc aataacattg atatgctgat tccgcgggat ttgcgtcctg
     4261 cgcatcctca atacattcgt cacaaccgtg aaggtggtaa agctcgcgtc gaggggatga
     4321 gtcgggagct gcagctggag aaaaaagacg gcagtaaagt ctggacccgt tttgcgctat
     4381 cgaaagtgag cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag
     4441 aaatggcgca aaaagaacag acccgacaat tgattattgc cgttgaccat ctcgaccgac
     4501 cggtgattgt cctcgatccg gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa
     4561 tgtttggtta ctgcattaac gaagccagcg gtatgcagcc cgatacactc ctgaacattc
     4621 cagaattccc tgctgataac cgtattcgtt tacaacagtt gctatggaaa accgcccgcg
     4681 atcaggacga atttctgctg ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta
     4741 tcagcccggt ttatgacgtg ctcgcgcatc tgcagaacct ggtaatgaca ttctcggata
     4801 tcacagaaga aaggcaaatc cgccagcttg aaggcaatat tctcgccgcc atgtgcagca
     4861 gcccgccatt tcatgaaatg ggggaaatca tttgtcgtaa catcgaatgt gttctcaacg
     4921 aatcgcatgt ttcgctgttc gcgctgcgca acgggatgcc gatacactgg gcgtcatctt
     4981 cgcacggtgc agaagttcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgcgatg
     5041 gcgcgcctgc ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta
     5101 tcgaacgcgt ggcagatatc agccagcata tggccgcgct ggcgctggaa caggaaaaaa
     5161 gccgtcagca tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca
     5221 ataacctgca caattacctc gatgacctgg tcgacaaagc cgtctctccg gtggtgtatc
     5281 tcatcggtgt tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc
     5341 aggcattgct ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct
     5401 gtcgtattga aggtgcgtct tttgtcctcg tgagcctgga aaacgatgtc agtaacatta
     5461 cccagatcgc cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacgataaac
     5521 cattcccgct caccttgagc attggcatca gctacgacgt gggtaaaaac cgcgattacc
     5581 tgctctccac tgctcacaat gcaatggatt ttattcgcaa aaatggcggt aacggctggc
     5641 agttcttcag cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc
     5701 taaaagaagc gattagcaat aaccaactta aactggttta tcagccgcaa atcttcgcag
     5761 aaacgggtga actgtacggc atcgaagccc ttgctcgctg gtacgatccc ctgcatggtc
     5821 atgtgccccc ttcacggttt attcctctcg cagaagagat tggtgaaatc gaaaatattg
     5881 ggcgctgggt catcgcggaa gcttgccgtc agttagcaga atggcgtagc cagaatattc
     5941 atatcccggc gttatccgtt aacttgtctg cgctgcactt tcgcagtaat cagctgccta
     6001 atcaggtatc tgatgcaatg cacgcctggg gtattgacgg ccaccagctg acggtggaaa
     6061 tcacggaaag catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc
     6121 gcgatatggg catcggttta tcggtagatg attttggtac aggcttttcc ggattatccc
     6181 gcttagtcag tcttccggta acggaaatca aaattgataa aagttttgtc gatcgttgtc
     6241 tgaccgaaaa acgcatcttt gccttactcg aagccattac cagcattggg caaagcctca
     6301 atttaaccgt cgtggctgaa ggcatcgaaa ccaaagagca atttgagatg ctacgcaaga
     6361 tccactgtcg cgttattcag ggatatttct tttcccgccc cctgcccgcc gaagaaattc
     6421 caggctggat gagcagcgtg ttaccgctga aaatctgata aattcctctc gcccgcactc
     6481 gcgggttttt atttaacgtg acactgtcac ttaaatactg tgatttccgc tacgtttagt
     6541 tggacaacga caacgcatag ccatgtcaca acaatccctt ctcagcctga tgtgattgtg
     6601 ctgacgcatc aggcattcac gcgcctgacg cgtcatccgg caacgcttta cgatttcaac
     6661 tggtaatcca gcgtaatttc cgctttcagt acctgagaaa ccgggcatcc tgcttttgct
     6721 ttctggataa tgccgtcaaa ggtggaggca tcaatacccg gcaccgcaac ttcgctcttc
     6781 agtgcgattt tagtaatcgc aaaaccggca tccactttat ccagcgagac atcagcggtg
     6841 gtatcaatcg atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag
     6901 aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca
     6961 aaacgcgtgt taaatccata cggttgttgg ttcagcacgc cgctctcagt agatactgtt
     7021 cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt
     7081 gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat
     7141 aagcaggata atccgaataa aatccggcga aaatataatt cctgcactta aattaaattt
     7201 ccacaataga taaccggaaa tatataactc aagagatatt gcatcaatat caatcaactc
     7261 atcacaataa taatacctat caatatcatg gagttaaaat ataaaaatca tcaaccagga
     7321 ctaatcttaa caacgaagcg gcaaatattt gccatgctga atgtgcttta taaatctgcg
     7381 atccgtagca gacaccataa atacacagac acggagaatc actatgttta cttattatca
     7441 ggcagaaaat tcaacagcag aacccgctct ggttaatgcc attgagcaag gccttcgggc
     7501 agagcacggt gttgtcactg aagatgacat tctcatggag ctgaccaaat gggtggaagc
     7561 ttctgataac gacatcctca gtgatatcta ccagcaaacg atcaattatg tggtcagtgg
     7621 tcagcaccct acgctttaag gtgctatgct tgatcggcaa cctaatttag gggtttagca
     7681 cgtgtttctt cgctacggcg atgttgtcct taaaactagc aacaggattg aggagttaaa
     7741 atgaaatcga accgtcaggc acgtcatatt cttggactgg accataaaat ttctaaccag
     7801 cgcaaaatag ttaccgaagg tgacaaatcc agcgtggtaa ataatcccac cggcagaaaa
     7861 cgccccgctg aaaagtaatt cataaccatc agtcctcaat gacgattaaa caccattgcc
     7921 tgcgcaatgg tgtttttgtt tttatctgct ttatacttgg tacttgacgc cctggcggta
     7981 atgcaaagac gataaaagcc cccagggatg gatattcaaa aaagagtgag tgacatggaa
     8041 ccaaaaacaa aaaaacagcg ttcgctttat atcccttacg ctggccctgt attgctggaa
     8101 tttccgttgt tgaataaagg cagcgccttc agcatggaag aacgccgtaa cttcaacctg
     8161 ctggggttac tgccggaagt ggtcgaaacc atcgaagaac aagcggaacg agcatggatc
     8221 cagtatcagg gattcaaaac cgaaatcgac aaacacatct acctgcgtaa catccaggac
     8281 actaacgaaa ccctcttcta ccgtctggta aacaatcatc ttgatgagat gatgccagtc
     8341 atttacaccc caaccgtcgg cgcagcctgt gagcgttttt ctgagatcta ccgccgttca
     8401 cgcggtgtgt ttatctctta ccagaaccgc cacaatatgg acgatattct gcaaaacgta
     8461 ccaaaccata atatcaaagt gattgtggtg actgacggcg aacgtattct ggggcttggt
     8521 gaccagggca tcggcgggat gggcattccg atcggtaaac tgtcgctcta taccgcctgt
     8581 ggcggcatca gtccggccta tacccttccg gtggtgctgg atgtcggcac caacaaccaa
     8641 cagctactta acgatccgct gtatatgggc tggcgtaatc cgcgtatcac tgacgacgaa
     8701 tactatgaat tcgttgatga atttatccag gctgtgaaac aacgctggcc ggacgtactg
     8761 ttgcagtttg aagacttcgc acaaaaaaat gcgatgccgt tacttaaccg ctatcgcaat
     8821 gaaatttgtt cttttaacga tgacattcag ggcaccgcgg cggtaacagt cggcacactg
     8881 atcgcagcca gccgcgcggc aggtggtcag ttaagcgaga aaaaaatcgt cttccttggc
     8941 gcaggttcag cgggatgcgg cattgccgaa atgatcatcg cccagaccca gcgcgaagga
     9001 ttaagcgagg aagcggcgcg gcagaaagtc tttatggtcg atcgctttgg cctgctgacc
     9061 gacaagatgc cgaacctgct gcctttccag accaaactgg tgcagaagcg cgaaaatctc
     9121 agtgactggg ataccgacag cgatgtgctg tcactgctgg atgtggtgcg taatgtaaaa
     9181 ccagatattc tgattggcgt ctcaggacag accgggctgt ttacggaaga gatcatccgt
     9241 gaaatgcata aacactgtcc gcgtccgatc gtgatgccgc tgtctaaccc gacgtcacgc
     9301 gtggaagcca caccgcagga cattatcgcc tggactgaag gtaacgcgct ggtcgccact
     9361 ggcagtccgt ttaatccagt ggtatggaaa gataaaatct accctatagc ccagtgcaac
     9421 aacgccttta ttttcccggg catcgggctg ggtgttattg cttccggcgc gtcacgtatc
     9481 accgatgaga tgctgatgtc ggcaagtgaa atgcttgctc agtattcgcc gctggtcctg
     9541 aacggcgaag gtctggtact accggaactg aaagatattc agaaagtctc ccgcgcaatt
     9601 gcctttgcgg ttggcaaaat ggcgcagcag caaggcgtgg cggtgaaaac gtctgccgaa
     9661 gctctgcaac aggccattga tgataatttc tggcacgccg aataccgcga ctaccgccgt
     9721 acctccatct aagcctgcgc ccggtagtga aggctaccgg gcaattttcc tctccctttt
     9781 tcagatctca tccatactgg gtagtggcga ataaatctca tttgcctcac ctgctatgca
     9841 gaacatcatc cgaaaaggag gaactatgaa ggctgcagtt gttacgaagg atcatcatgt
     9901 tgacgttacg gataaaacac tgcgctcact gaaacatggc gaagccctgc tgaaaatgga
     9961 gtgttgtggt gtatgtcata ccgatcttca tgttaagaat ggcgattttg gtgacaaaac
    10021 cggcgtgatt ctgggacacg aaggtattgg tgtggtggca gaagttggcc ctggtgttac
    10081 ttcattaaaa ccaggcgatc gtgccagcgt ggcgtggttc tacgaaggat gcggtcattg
    10141 cgaatactgt aacagtggta acgaaacgct ctgccgttca gttaaaaatg ccggatacag
    10201 cgttgatggc gggatggcgg aagagtgcat cgtggtcgcc gattacgcgg taaaagtgcc
    10261 agatggtctg gactcggcgg cggccagcag cattacctgt gcgggggtca ctacctacaa
    10321 agccgttaag ctgtcaaaaa ttcgtccagg acagtggatt gctatctacg gtcttggcgg
    10381 tctgggtaac ctcgccctgc aatacgcgaa gaatgtcttt aacgccaaag tgatcgccat
    10441 tgatgtcaat gatgaacagt taaaactggc aaccgaaatg ggtgcagatt tagcgattaa
    10501 ctcacgcacc gaagacgccg ccagaattgt gcaggagaaa gccggtggcg ctcacgcagc
    10561 ggtggtgaca gcggtagcta aagctgcgtt taactcggcg gtggatgctg tccgtgcagg
    10621 tggtcgtgtc gtggctgtcg gtctgccgcc ggagtctatg agcctggaca tcccacggct
    10681 ggtgctggat ggtattgaag tggtcggttc gctggtcggc acgcgtcagg atctaaccga
    10741 agccttccag tttgccgccg aaggcaaagt ggtgccgaaa gtcgccctgc gtccgttagc
    10801 ggacatcaac accatcttta ccgagatgga ggaaggcaaa atccgtggcc gtatggtgat
    10861 tgatttccgt cgttaagagg tctttgctgc gactgccatg ttcgggtcgc agcctgtttt
    10921 tcgagacgct ttccgcaatt tcactcttct tcttttcctc ccccatcttt cccctcacaa
    10981 ttcgcgaaaa cgtagcgaaa tttgttgcgc tgcacggaac aatagtgata gtgttccttt
    11041 cgagggaaca aggatgaaga gatgataatg aatttcagac acaagggatt gcgtgacttg
    11101 tttcttctcg gcaaaacctc cggcgttata ccgacgcaag tcaaacgatt acgccaccga
    11161 ctcgctgtga ttgatgcagc atgctgtctt gctgatatcg atatgcccgg ttaccgacta
    11221 catccgttaa gcggcgatcg cgatggaatt tgggcgatat ctgtctcggg caactggcga
    11281 atcacatttg aattcgtcaa tggcgatgca tacatactgg attacgagga ctatcactga
    11341 tgaaaatggc caatcatccc cgcccggggg acattattca ggaatcactg gacgaactta
    11401 atgtcagcct gcgcgagttt gccagagcaa tggaaattgc gccctcaacg gcaagccgat
    11461 tgctgaccgg aaaagcagct ttgacgccag aaatggcaat aaaactttcc gtggtgatcg
    11521 gcagttcgcc gcaaatgtgg ctgaatctgc aaaatgcctg gagtctggca gaggcagaaa
    11581 aaacagtgga tgtgtcgcga ctgcgccgtc tggttacgca ataaaaaaat ggcaccttat
    11641 aaagcgccat ttgaaactga tgacaaacgc aacattgccc gatgcgctac gcttatcagg
    11701 cctacacgtt cactgcaatc tcctggattt gaacggtttg taggccggat aaggcgttca
    11761 cgccgcatcc ggcatgaaca aagtgcacgt tgtctacaat aaaaaaatgg cgccttttac
    11821 agcgccattt tcagcttaaa gctcaaaaga gattataacc cttcttcact ctcttttttc
    11881 gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc ccagcgacgg
    11941 ctcaccttcc cttcgatcat ccctttaata gatcctttca cccagaatgc catatacata
    12001 tggatcagga tggcgtggat caggatgata cccgcagccg catggatcag caggctataa
    12061 cggacaacct gtattgggaa gtactgcgca aagtacggac gccagataat cactccggtc
    12121 accagcagca cgaaaatcat gctcatgatt gaccagaaca tcatcttttg cccggcgttg
    12181 tacttaccga catccgccac tttgtgctca ttgcctttta atacttcgac aatgttcaac
    12241 agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac aaacataaac
    12301 atcagcgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc catctgcggc
    12361 gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat cccggacagc
    12421 gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat aaatttggtg
    12481 cgcacaatca ttttcgactt actcatgatt tccctcctcg tcatcgtcca cttccttatt
    12541 cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc
    12601 tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtatcgatct tcggatcttt
    12661 cggcagacca tgatacagct ccggctgatc ggcatgatgc agcacgtaca taacgtgtgt
    12721 accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg ctttcagttt
    12781 cgctacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt ggatagcccc
    12841 ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat cgacgcagag
    12901 cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa acggacaccc
    12961 ggcaatgcag taaccacagc cgatgcaatt ttccgactgg aaatcgacaa tcccgttagc
    13021 gtactgaatg attgcaccag cagacgggca cgccttcagg cagcccggat cttcacagtg
    13081 catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg tttcgctaaa
    13141 gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca
    13201 gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac aggctttaca
    13261 gccgatacag gtggaaacgt cgataagttt cgctacttct gctttgtaat cacgcacctg
    13321 agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg tttccatagc
    13381 catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat attccggcgt
    13441 ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac ctttacgcgc
    13501 gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct gaccgtttac
    13561 attcagcggt ttcagacggc gcgttaccac agccaccgcg cggataaagc cacgcttgct
    13621 ggagacagtg acacgatcgc cattggcaat gcctttcgcc gccgccagcg tttcgctgat
    13681 ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg tccaggtgtg
    13741 gaagtgctcc gtcagacgat aggtcgtacc cacatacggg aactgctctt ttttacccat
    13801 ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt tcggatgcaa
    13861 cgggttagtg cccagcggcg tttcaattgg ctcgtagtgt tccgggaacg gaccttccgc
    13921 cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa acggcccggt
    13981 tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc acttgctacc
    14041 gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat ccgccgaagc
    14101 acggttgtag agtacgcgac ggttgagcgg ccacgcccag gcccatccca gcgtattccc
    14161 cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct cggtccagct
    14221 accggtgtag atccagcaag aagatgcggt tgtaccgtcg tcacgcagat gcgcaaagct
    14281 actcagcaac tgacctttct tcgcaatcag cacgccatta gcgtcataga gatcttccag
    14341 cgcatagccg ttgttctctt tcgccacttc gtcagactgc ggttcgtgcg gctgcttgta
    14401 gttccagctc atcttcatca gcggttctac gcctttacca ccctcggcct ggtacagctc
    14461 gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgtgctt cgcccggcgc
    14521 gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag aaccatcttc
    14581 ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagatg ccggatcgac
    14641 atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca gcggatcgat
    14701 aaccaccatg tacttcagtt tgctcaggca gctcaccact ttgtttttgt ccgggaagga
    14761 cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcgt ccatcatgtt
    14821 gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcgt agccccagtt
    14881 gttctctttc tgcgcggcat cgccgtagaa agatttcatc aggctaacga agaacttcgg
    14941 atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtat tcgcttccag
    15001 atacgactgc aaatcaacct gtttttctga cggtagcgtc agataacctg gcaggctggt
    15061 agagagcagg cctaagtcag tcaggccctg aatgttggag tgaccacgca atgcgttcac
    15121 gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca tcgtacggat
    15181 gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg tggttgtgcg
    15241 atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg cttttggcgt
    15301 accgcaaatg ttttctacta cgtccggcgt gtagcgggaa acgtgcgctt tcagcagatt
    15361 ccatacacag cgcggatgag tcagcgtgtc atcgcgtttc gcatagccgt tttcatcgaa
    15421 ctgatagttc caggacgact tatcgtattg acgtttttca gcgtcgtagc cgctgaacag
    15481 accgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt aatgcttaac
    15541 gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag acaggaacgt
    15601 aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg tacgcgtaaa
    15661 acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca tcgcccagcg
    15721 gaaaccgacg ggatgcgctt cagcagcgtt accgcccatc accattacga cgttagcgtt
    15781 tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc
    15841 taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc cgagggagcg
    15901 ggcaaatttt tgagtcagca tcccggtttc gttgctggca ccggaagcac acagcatacc
    15961 ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa agttagcgtc
    16021 acggtcggct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc taatgcgctg
    16081 ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt cgctgttgac
    16141 gtaatccagc aaaccggccc ctttcgggca cagcgcacca cggcttaccg gatgatccgg
    16201 gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac ccaggctata
    16261 catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct ctttcgcgcg
    16321 taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc ccaatgctgc
    16381 tactgttgtt cccgccatac cgcccgcgca gattttaaaa aattgtctgc gactgacgtc
    16441 cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag taaaattctg
    16501 aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc cgcctgcgca
    16561 aattgaccta cctcaatagc ggtagaaaaa cgccccactg cctgacaggg cagttaaaaa
    16621 aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc gagcatgaca
    16681 cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac gatggtagga
    16741 ttgattcgcg gtgtcagtga gggactcggc ccggtcggcg gcgcagctgc tatctattca
    16801 ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcgaca gatcccgaaa
    16861 ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct ggcgctttcc
    16921 ttagggtatg cggcgacccg tcatcaggcg attgaagtgg ggatggtgaa ctatctatgg
    16981 cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa ctggctgatt
    17041 gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg tgacaatggg
    17101 ttacattatg atcaaattat taataatatc accaccagcc cattgagtta tttcctggcg
    17161 ttcattgggg cgtttatctg ggcagcctac tgcacagtaa cgaataaata cgcacgcgga
    17221 tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt ttactatttt
    17281 cttacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact catctctgcg
    17341 gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattaca tggcaacgtc
    17401 accataatgg cggtaggttc gtattttacg cccgtccttt cctcagcgct tgcagccgtg
    17461 ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt ctgcggcggt
    17521 tccctgctct gctggctggc gacacgtcgt ggctaaaata attgtcgggt ctacggcccg
    17581 gcagttaatc agataaattc tgacaatatt aaaaaataaa tttcaaatac cagaaagtca
    17641 ttttagttac atttagtaac acatatctgt ctgtttaatc catattctgc ccccgcagtt
    17701 acgctttctt atctattctt ctaatcagca cttcacctga ttcatcctta attctaataa
    17761 ctggctattg cgccttacct cctgttttgt ctttttttca ttaccaatgt gtgcatgtga
    17821 ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc gactggaaac
    17881 cagaaaatcc ggccttctgg gaaaataaag gaaagcatat tgcacgaaga aacctctgga
    17941 tatcagtcag ttgcctactt cttgccttct gtgtctggat gctatttagc gcagttactg
    18001 ttaatcttaa taaaatcggt tttaatttca ctaccgatca actcttttta ttaaccgcat
    18061 taccctccgt ttctggcgca ttattgcgtg tcccctactc ctttatggtg cctatattcg
    18121 gtggacgccg atggacggtt tttagtactg caatcctgat tattccttgc gtctggctcg
    18181 gaattgccgt gcaaaatccg aatacccctt ttgggatatt tatcgttatc gctttgctat
    18241 gcggttttgc aggtgcgaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa
    18301 aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg
    18361 tgatgcagct ggttgcaccg ctggtcattt ttgtacccgt atttgccttt ctcggcgtca
    18421 atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg
    18481 tgccattact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt
    18541 cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga
    18601 gcctgcttta tcttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc
    18661 tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta
    18721 tcggtgccat cgcgcgatcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg
    18781 tgacgttgat caacttcatt tttatggcga ttttcagcgc cctgctgttc cttaccttac
    18841 cgggcacagg ctccggtaat tttatcgcat tttacgccgt atttatgggg ctgtttctga
    18901 ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa
    18961 caatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcaaaga gaagccgtca
    19021 ccgaaacggc agccgctctg ggctttatct cagccattgg cgcagtgggc ggctttttta
    19081 ttccgcaggc gtttggcatg tcgctcaata tgaccggctc tccggtgggc gcgatgaaag
    19141 tgtttttaat cttctacatc gtttgtgtgc tactgacctg gctggtttat ggtcggcgga
    19201 agttcagcca aaaataagag atcgatgcct gacacgtctt atcaggctac aaccgtgtcg
    19261 catccgacaa ggcatcagat cccaattcat tcctggagca ggagttatgt catgagtaaa
    19321 cttttggatc gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga
    19381 caggtgatgc atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac
    19441 aaaatcgtgc gttccaccca cggtgttaac tgtacaggct cctgtagctg gaaaatctac
    19501 gttaaaaatg gtctggtgac ctgggaaatc caacagaccg actacccgcg cactcgccct
    19561 gacctgccaa atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctc
    19621 tacagcgcta accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc
    19681 gaagccctca agcaacacag cgatccggta ctggcgtggg catcgattat gaacgatccg
    19741 caaaagtgcc tgagctacaa acaagtgcgt ggacgcggtg gttttatccg ctccaactgg
    19801 caggagctaa atcagctgat tgccgctgct aacgtctgga ccatcaaaac ttacggcccg
    19861 gatcgcgttg ccggattctc accgattccg gcgatgtcga tgatttctta cgccgccgga
    19921 acacgctatc tgtcgctgtt aggtggcacc tgtttaagct tctacgactg gtattgcgac
    19981 ctgccgcccg cctcgccgat gacctggggc gagcaaaccg acgtaccgga atctgccgac
    20041 tggtataact ccagttacat catcgcctgg gggtctaacg taccgcagac acgtacgccg
    20101 gacgcccatt tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct
    20161 gactactctg aagtggccaa attgtgcgac cagtggctgg caccgaaaca aggcactgat
    20221 agtgccctgg caatggcaat gggccatgtc attttaaaag agtttcatct cgataatccc
    20281 agcgattatt ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg
    20341 gaacctcgcg acgatggcag ctacgttccc gggcgcatgg tccgcgcatc tgacctggtg
    20401 gatggactgg gcgaaagcaa taatccgcag tggaaaaccg tggcagttaa taccgcaggt
    20461 gaactggtgg tgccgaacgg ttcgattggt tttcgctggg gagaaaaagg caaatggaac
    20521 ctggaatcca ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacac
    20581 gacaccgtcg caggcgtggc cttcccctac tttggcggca ttgaaaatcc acattttcgc
    20641 agcgtaaaac acaatcctgt cctggtgcgc caattgcccg ttaaaaacct gacattagcc
    20701 gatggtagca cctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc
    20761 gatcgcgggc tggaagatga aaacagcgcg aaagattacg ctgaaatcaa accttatacc
    20821 cctgcctggg gcgagcaaat taccggcgtt ccgcgccagt atattgaaac catcgcccgt
    20881 gaatttgccg ataccgccca taaaacgcat gggcgctcga tgattattct cggcgcaggt
    20941 gttaaccact ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc
    21001 tgcggttgtg tcgggcaaag cggtggcggc tgggcacact atgtcggtca ggaaaaactg
    21061 cgcccgcaaa ccggctggct gcctctggcc tttgcgctcg actggaaccg accaccgcgc
    21121 cagatgaaca gcacctcgtt tttctacaat cactccagcc agtggcgcta tgaaaaagtc
    21181 accgcgcagg aattgctctc accgctcgcc gatgccagta aatacagcgg tcacctgatt
    21241 gatttcaacg ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gttggggcgt
    21301 aatccgctcg ggattaaagc tgaagccgac aatgccggat tatctcccac agaatttacc
    21361 gcccaggcgc tgaaatcggg cgatttacgt atggcctgcg aacaaccaga tagcggcagc
    21421 aatcatccgc gtaatttgtt tgtctggcgt tctaacctgc ttggctcctc cggcaaaggc
    21481 cacgagtata tgcagaagta tctgctggga accgaaagtg ggattcaggg tcaggaactc
    21541 ggtgccagcg acgggatcaa accagaagaa gtcgagtggc aaactgcggc gattgaaggc
    21601 aagctcgacc tgctggtgac gctcgacttc cgtatgtcca gtacctgcct gttctccgat
    21661 atcgttctgc ccaccgccac ctggtacgaa aaagacgata tgaatacctc ggatatgcat
    21721 ccgtttattc atccgctttc tgcggcggtc gatccggcct gggaatcacg cagcgactgg
    21781 gaaatctaca aaggtatcgc taaagccttt tcgcaagtgt gcatagggca tctcggcaaa
    21841 gaaaccgacg tggtattaca accactgctg catgactccc cggcagagct ttcacagccg
    21901 tgtgaagtgc tcgactggcg caaaggcgaa tgcgatctga ttccaggcaa aaccgcgccg
    21961 aatattgtgg cggtggagcg cgactaccct gctacctatg aacgctttac ctcgctcggg
    22021 ccattgatgg acaaacttgg caacggcggt aaagggattt cgtggaatac ccaggatgaa
    22081 attgatttcc tcggtaaact taattacacc aagcgtgatg gcccggcaca ggggcgtccg
    22141 ctgattgata ccgcgattga cgcatcagaa gtgattctgg cgctggcacc ggaaaccaac
    22201 ggccatgttg cagtcaaagc gtggcaggcg ctgggcgaga tcaccggacg cgaacatacc
    22261 catctggcgc tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg
    22321 cgtaaaatta tctccagccc cacatggtct ggtctggaaa gcgatcacgt ttcctataac
    22381 gcgggataca ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcaa
    22441 ctctatcagg atcatccgtg gatgcgtgct tttggtgaaa gtctggtggc ttatcgcccg
    22501 cctatcgaca cccgtagcgt cagtgaaatg cgccagatac cgccaaacgg cttcccggaa
    22561 aaagcactta acttcctgac gccgcaccag aaatggggca ttcactcaac ctacagtgaa
    22621 aacctgctaa tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaaccgat
    22681 gcccgagaac tgaccattgt cgataacgac tgggtggaag tatttaacgc caacggcgcg
    22741 ctgactgccc gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac
    22801 gcccaggaac gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt
    22861 cataactcgg tcacccgcgt ttgtccgaaa ccaacgcata tgattggcgg ttacgcgcag
    22921 ctggcctggg gctttaacta ctacggcact gtcggctcta accgcgacga attcatcatg
    22981 atccgcaaga tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggttcaggag
    23041 gcgaaaaaat gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg
    23101 gctgccatac ctgttcggtg acctgtaaaa acgtctggac cggacgcgaa ggcatggagt
    23161 acgcatggtt taacaacgtc gaaaccaaac cgggcattgg ttacccgaaa aactgggaag
    23221 atcaggatga gtggcaaggc ggctgggtgc gtgatgtgaa tggcaagata cgcccgcgtc
    23281 tgggcagcaa aatgggcgtg attaccaaaa ttttcgccaa cccggtagtg ccgcagattg
    23341 acgattacta cgaacctttc accttcgact acgaacattt gcatagcgcg ccggaaggca
    23401 aacatattcc caccgcccgt ccgcgttcac tgattgacgg caaacggatg gacaaagtga
    23461 tctggggccc aaactgggaa gaactgctgg gcggcgaatt tgaaaaacga gcccgcgacc
    23521 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
    23581 acctgccgcg cttgtgcgaa cactgcctta accccagttg tgtagcgacc tgccccagcg
    23641 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat cgatcaggat aaatgccgtg
    23701 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
    23761 gcaagtcaga aaaatgtatc ttctgttacc cacgaattga gtcaggccaa cccaccgtgt
    23821 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
    23881 gcattgagga agcggcaagc accgagcgcg aagttgacct ctatgagcgc cagtgcgaag
    23941 tgttcctcga tccacacgat ccctcagtga tcgaggaagc cctgaaacaa ggtattccac
    24001 aaaacgtgat tgacgctgcc cagcgttcgc ctgtctacaa aatggcgatg gactggaaac
    24061 tggcgctacc gctgcaccct gaatatcgca ccctgccgat ggtctggtac gttcctccgc
    24121 tgtcaccgat tcagtcctac gctgatgcgg gaggtttgcc gaaaagcgaa ggcgtgctgc
    24181 ccgccatcga aagcctgcgt attccggtgc aatatctcgc caatatgttg agtgccggtg
    24241 ataccggtcc ggtactgcgg gcgctgaaac ggatgatggc gatgcgccac tatatgcgtt
    24301 cacaaaccgt ggaaggcgtt actgatactc gtgccatcga cgaagtaggt ctgagcgtcg
    24361 cccaggtcga agagatgtat cgttacctcg ccattgccaa ctatgaagat cgtttcgtca
    24421 tcccgacaag ccatcgggaa atggcgggcg atgccttcgc agaacgcaac ggctgcggtt
    24481 ttacctttgg cgacggttgc cacggttcgg acagcaaatt caacctgttc aacagtagcc
    24541 gtatcgatgc catcaacatc accgaagtgc gcgacaaagc ggagggcgaa taatgcagat
    24601 cctcaaagtg atcggcttgt tgatggagta tccggacgag ctgttatggg aatgcaagga
    24661 ggacgcgctg gcgttgatcc gccgcgacgc accgattctt acggatttta cccgcaacct
    24721 gcttaacgcg ccgctgctgg ataaacaggc cgaatggtgc gaagtgtttg accgtggacg
    24781 taccacatcg ctgctgctgt tcgaacatgt tcatgccgag tcccgcgatc gcggtcaggc
    24841 aatggtggac ctgctggcgg agtatgaaaa ggtcggtctg caactgaatt gtagggaact
    24901 gcccgattat ctaccgctgt atctggagta tttaagcgta cttccggacg atcaggcgaa
    24961 agaaggatta ctcaacgttg cgccgatcct cgccctgctt ggcggacgct taaaacaacg
    25021 cgaggcaccg tggtacgcgt tatttgacgc cctgttgcaa ctggcaggaa gccctctgtc
    25081 aagtgacagt gtcacaaaac agattcgcag tgaagagcgt gacgacaccc gccaggcgct
    25141 ggatgcggtg tgggaagaag aacaggtgaa gtttattgaa gataacgcca cggcgtgtga
    25201 cagctcgccg cttaatcaat atcagcgacg ctttagccag gatgtcgcgc cgcagtatgt
    25261 cgacatcagt gcgggaggtg ggaaatgatt cagtatctga acgtcttttt ttacgatatc
    25321 tacccgtact tgtgtggcac ggtttttatc cttggaagct ggctgcgcta cgattacggg
    25381 cagtatacct ggcgtgccgc ctccagccaa atgctggata aacgcgggat ggtgctatgg
    25441 tcgaacttgt tccatatcgg cattctgggg atcttcttcg gtcatctgtt cggcatgtta
    25501 acgccgcact ggatgtacgc gtggtttctg ccggtggcgg taaaacagca gatggcgatg
    25561 atcctcggcg gaatatgcgg ggtattgacg ctgattggcg gtgcggggct actggtgcgt
    25621 cgtttaacaa atccgcgtat cagggtgacg tcttctggtg cggatatcct gatcctcgcc
    25681 attttgttaa ttcagtgcat tcttgggtta acccccattc ctttttcagc acaacatccg
    25741 gacggcagtg aaatgatgaa gctggtgggc tgggcgcaaa gcattgtcac tttccagggc
    25801 ggtgcatcta cgcatcttga tggtgtcgcc tttatatttc gcgttcatct ggtgcttgga
    25861 atgaccatct tcctgctctt cccgttcacc cgactggtgc atgtctggag cgcgccgttt
    25921 gagtacttta ctcgtcggta tcagattgtg cgttcgcggc gttaattctc acgcgggccg
    25981 gatagccctt cgctctccgg ccacttgcag gctatgatag ttctccagcc acaggagaac
    26041 attaatgaaa ccgcaggttt accacgtcga tgcttttacc tctcaaccat ttcgcggcaa
    26101 ttctgccggt gttgttttcc ccgccgataa tctcagtgaa gcgcaaatgc agcttatcgc
    26161 ccgcgagtta ggtcattcgg aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg
    26221 catccgctac tttacgccaa cggttgaagt tccgatttgt ggtcacgcga cagtagctgc
    26281 gcactatgta cgtgcaaagg tgttaggttt aggaaattgc acggtctggc aaacatcgct
    26341 ggcaggaaaa catcgtgtga ctatagaaaa gcaaaatgat gattatcgta tttcgctgga
    26401 acaaggtact ccaggctttg agccaccgct ggaaggtgaa acacgtgcag cgattatcaa
    26461 cgcgcttcat ctcactgagg acaatattct gccaggcttg ccaattcagg tggcaacaac
    26521 aggacactca aaagtgatga tcccactgaa gccggaagtg gatatcgacg ccctttcgcc
    26581 tgatcttgct gcgctgaccg ctatcagcaa acagattggc tgcaatggtt tcttcccgtt
    26641 ccagatccgc ccaggcaaaa gcgaaaccga cggtcgcatg ttctcgcctg cgattggtat
    26701 tgtggaagat ccggtgactg gcaatgccaa tggcccgatg ggtgcatggt tagtacatca
    26761 caacgtattg ccccacgatg gcaacgtgtt gcgtgttaaa ggccatcagg ggcgcgcatt
    26821 agggcgtgat ggtgtaattg atgtgacggt gacaattcga gataaccaac cggagaaagt
    26881 taccatttcc ggcacggcgg tgattctatt ccatgctgaa tgggccattg atttttgatg
    26941 ccgttgcctg atgcgcttcg cttatcaggc ctacaaccgg actaatgtag gccggataag
    27001 gcgtttacgc cacatccggc aacctgacat aaattatttt cccgcctccg ggtgcgtatc
    27061 aaacgccgcc atcaccagcg ctaactcatc cacagtaaag ccatgtttcg catcatctac
    27121 gcccagacca aactgttcct gcatcactgc atataacgac gccacatctg gtagatttcg
    27181 ctgctcaact gcgtgcccat tttcataatg ggtaaaatga aaattggtca gcgttagctt
    27241 gccaccgtcc ggcaaatggc gacacatcag caaatgatgg cgaaaatgcg actgcggcca
    27301 gtgcgccgac cagaaattgc ccatcacata atcgctttgt tgctgctcgc agagatcaaa
    27361 acggtacatc gactgccagt gctgatgatg attaaactgc aatacccaat catcaccctc
    27421 ttgcaacaac cgatactctc cgtgtggtgt ggtttgcatg atatccggca ttaaacgaat
    27481 cggcgcggtt agcgtctgcc caccgaaacc gacatcagca atccattttt ccccttccag
    27541 ttccaccaac agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg ataacactac
    27601 gcgacctaac aagctgcgaa cgttaaaccc cagctcgcgt aacacccgct caaacacgcc
    27661 attctgctca aaacagtaac cgccacgacg ggctatcacc agtttatctt ccagcgattg
    27721 atcatcaagc tgtatttccc tcggcagcaa aacgtcgaga ttttcaaacg gaatggtgca
    27781 attgtgtttc aggtgcaatg cacgcagcgt atcaatattg gccgcagcag ctcccaacca
    27841 gttaatacgg gcaaaataat gattcagaat gggcgtcatc aggcgtttcc cttagtggtt
    27901 ttctcaactt ataacctgga atagacgccg cactggctat tttgtgcgat cgcggacatg
    27961 catcacgttc gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt acgagctacg
    28021 cttttaaaac acccttcata gcgaggcgat atcgtgagcc aattcattcc cgtcgaatta
    28081 caccatgcca gccgtctgtt aaatcatggc ccaactgtca tgatcaccag tttcgatgag
    28141 caatcccagc ggcgtaacat tatggcagca gcctggtcga tgcccgtaga gtttgaaccg
    28201 ccacgcgtgg cgattgtggt agataaatcg acatggacca gggagttgat tgaacgtaac
    28261 ggtaagtttg gcatcgttat cccgggcgtt gcggcgacta actggacgtg ggcggtggga
    28321 agtgtgtcgg gtcgtgaaga agataaattt aattgctacg gcattccggt agtgagaggc
    28381 ccggtgcttg gtttgccgct ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgcctg
    28441 ctacctgcaa cttccgcgca agaaaaatac gatacgctat ttggcgaagt ggtatcagca
    28501 gcggcagacg cacgggtatt tgtcgaaggc cgctggcagt ttgatgatga taagctcaat
    28561 acgttgcatc atttaggtgc tggcacgttt gttaccagtg gtaagcgagt taccgcgggc
    28621 taaagaatca tatgcagcaa ccaagttgtt taagcaatgt ttccatatga gttaaagcat
    28681 attttatctc acgattgcat atttttagtg aagattaatt tatcctcacc aacatcatag
    28741 taattgcgta ttatcgcctc attgataaag ccctgttttg tataaaatgc gcgggcggca
    28801 ctaaaatcga tatggctggc ggtctctact aatagtagtc ttgcctgttt aattgtgagc
    28861 tcttttctat ttcgttaatc agcagatgtc tcactccctg gcgctgatgg gaaggactga
    28921 tccagagcat taataaattt cagacatcat ttgtcatcat ttcagaagca cagtaggcaa
    28981 tccctgccag tcccaagtgt tctgcactaa accataaatc ggcagattga cctgagaaat
    29041 aatgtgttag tgttttttgt acatgatgaa ggccatcttg atcaaattga tccctgtcaa
    29101 gcagcatatc cattaatggt tcaacatctg aaggtttgac tggtcgtata aacatggaac
    29161 tccttattta ttgatgaata tttgaaaatt gtttgcatgg aacgcggtta acctgaattt
    29221 tcagcggaag tttcactcaa aggctttatt aatccgctcc ttatgtcctt ttacagcaaa
    29281 taaataatta cctccctgtt tttgtatctt ct
//
LOCUS       DAZFRE010000065        29216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFRE010000065 DAZFRE010000000
VERSION     DAZFRE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.065"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(47..739)
                     /gene="ompL"
                     /locus_tag="ACHKUI_003162"
     CDS             complement(47..739)
                     /gene="ompL"
                     /locus_tag="ACHKUI_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO6599313.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(807..2210)
                     /gene="yihO"
                     /locus_tag="ACHKUI_003163"
     CDS             complement(807..2210)
                     /gene="yihO"
                     /locus_tag="ACHKUI_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599314.1"
                     /translation="MSNHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDATP
                     TGHKASILKSFYAIFSNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            complement(2253..3638)
                     /gene="yihP"
                     /locus_tag="ACHKUI_003164"
     CDS             complement(2253..3638)
                     /gene="yihP"
                     /locus_tag="ACHKUI_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599315.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(3684..5720)
                     /gene="yihQ"
                     /locus_tag="ACHKUI_003165"
     CDS             complement(3684..5720)
                     /gene="yihQ"
                     /locus_tag="ACHKUI_003165"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO6599316.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQTELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            complement(5826..6701)
                     /locus_tag="ACHKUI_003166"
     CDS             complement(5826..6701)
                     /locus_tag="ACHKUI_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6599317.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            complement(6762..7664)
                     /gene="yihR"
                     /locus_tag="ACHKUI_003167"
     CDS             complement(6762..7664)
                     /gene="yihR"
                     /locus_tag="ACHKUI_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO6599318.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            complement(7731..8972)
                     /gene="yihS"
                     /locus_tag="ACHKUI_003168"
     CDS             complement(7731..8972)
                     /gene="yihS"
                     /locus_tag="ACHKUI_003168"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO6599319.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(8988..9866)
                     /gene="yihT"
                     /locus_tag="ACHKUI_003169"
     CDS             complement(8988..9866)
                     /gene="yihT"
                     /locus_tag="ACHKUI_003169"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO6599320.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(9890..10786)
                     /gene="yihU"
                     /locus_tag="ACHKUI_003170"
     CDS             complement(9890..10786)
                     /gene="yihU"
                     /locus_tag="ACHKUI_003170"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6599321.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            10954..11850
                     /gene="yihV"
                     /locus_tag="ACHKUI_003171"
     CDS             10954..11850
                     /gene="yihV"
                     /locus_tag="ACHKUI_003171"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO6599322.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNEAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHEGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            11884..12669
                     /gene="csqR"
                     /locus_tag="ACHKUI_003172"
     CDS             11884..12669
                     /gene="csqR"
                     /locus_tag="ACHKUI_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6599323.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            12768..13367
                     /gene="yihX"
                     /locus_tag="ACHKUI_003173"
     CDS             12768..13367
                     /gene="yihX"
                     /locus_tag="ACHKUI_003173"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6599324.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            13361..14233
                     /gene="yihY"
                     /locus_tag="ACHKUI_003174"
     CDS             13361..14233
                     /gene="yihY"
                     /locus_tag="ACHKUI_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6599325.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            14230..14667
                     /gene="dtd"
                     /locus_tag="ACHKUI_003175"
     CDS             14230..14667
                     /gene="dtd"
                     /locus_tag="ACHKUI_003175"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6599326.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDADGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            14712..15653
                     /gene="fabY"
                     /locus_tag="ACHKUI_003176"
                     /gene_synonym="yiiD"
     CDS             14712..15653
                     /gene="fabY"
                     /locus_tag="ACHKUI_003176"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6599327.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(15717..16625)
                     /locus_tag="ACHKUI_003177"
     CDS             complement(15717..16625)
                     /locus_tag="ACHKUI_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6599328.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            16854..17165
                     /locus_tag="ACHKUI_003178"
     CDS             16854..17165
                     /locus_tag="ACHKUI_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6599329.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            17166..17456
                     /gene="nadS"
                     /locus_tag="ACHKUI_003179"
     CDS             17166..17456
                     /gene="nadS"
                     /locus_tag="ACHKUI_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6599330.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            17815..18093
                     /locus_tag="ACHKUI_003180"
     CDS             17815..18093
                     /locus_tag="ACHKUI_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599331.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            18489..18707
                     /gene="yiiE"
                     /locus_tag="ACHKUI_003181"
     CDS             18489..18707
                     /gene="yiiE"
                     /locus_tag="ACHKUI_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6599332.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(18923..19852)
                     /gene="fdhE"
                     /locus_tag="ACHKUI_003182"
     CDS             complement(18923..19852)
                     /gene="fdhE"
                     /locus_tag="ACHKUI_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6599333.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(19849..20484)
                     /gene="fdoI"
                     /locus_tag="ACHKUI_003183"
     CDS             complement(19849..20484)
                     /gene="fdoI"
                     /locus_tag="ACHKUI_003183"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6599334.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(20481..21383)
                     /gene="fdoH"
                     /locus_tag="ACHKUI_003184"
     CDS             complement(20481..21383)
                     /gene="fdoH"
                     /locus_tag="ACHKUI_003184"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6599335.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(21396..24446)
                     /gene="fdnG"
                     /locus_tag="ACHKUI_003185"
     CDS             complement(21396..24446)
                     /gene="fdnG"
                     /locus_tag="ACHKUI_003185"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23859..23861),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6599336.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            24640..25473
                     /gene="fdhD"
                     /locus_tag="ACHKUI_003186"
     CDS             24640..25473
                     /gene="fdhD"
                     /locus_tag="ACHKUI_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6599337.1"
                     /translation="MKKTQQKEIENVTSVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            25561..26115
                     /locus_tag="ACHKUI_003187"
                     /pseudo
     CDS             25561..26115
                     /locus_tag="ACHKUI_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
     gene            26469..27863
                     /locus_tag="ACHKUI_003188"
     CDS             26469..27863
                     /locus_tag="ACHKUI_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6599338.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
     gene            complement(27904..28218)
                     /gene="rhaM"
                     /locus_tag="ACHKUI_003189"
     CDS             complement(27904..28218)
                     /gene="rhaM"
                     /locus_tag="ACHKUI_003189"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6599339.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(28228..29052)
                     /gene="rhaD"
                     /locus_tag="ACHKUI_003190"
     CDS             complement(28228..29052)
                     /gene="rhaD"
                     /locus_tag="ACHKUI_003190"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6599340.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIIHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
BASE COUNT         7242 a         7204 c         7540 g         7230 t
ORIGIN      
        1 tgtgtaaaaa gccgcttaaa tatcagcggc tttttacatt ttcatatcag aagaaatact
       61 tcgttccgat acgaatctgg ttttgctcgc ggtggtacgg ctcgacgttg cggtctaacc
      121 agcgcaattc gaaataagga agccagtgtt cattaatacg gtaacggaag gtgttagtga
      181 tttcccaatg atgatctttc ccgttgctac tattaaagtc attcactcgc atgaagtaat
      241 gtggttcaaa tgtataggaa aatttttctg ttattttaaa gttccagtag gtgccaactt
      301 cataggtgtc gttattatcc aattcgccgt tcaaatctgt agagctgtaa ttattatggt
      361 tatagcgatt acgcaccgtc aggttaaacc acggagtaaa tttgtagtta acatccagat
      421 aaacagcacc acccgaaccg atactcttgt cattaattaa gccaccgggc tggatggtta
      481 atttatcggt aggtttaaat aaaggatacc aaccttcaat ttcattatag ccatgtttga
      541 gttcatcttc tcgctgaaag gtgtaggtat tggttagcat aatacccgcg cccatatcga
      601 agttataacc cacacgtagc ataacctcac cctgatcgga ggcgagatta tatgcttccc
      661 tgttttcgac gtatgcgcca gcgaataccg aggcagaggt cagagaagat aataaaatta
      721 ttgcattaat atttttcata ataagtccat tttttaaacg tagggaggcc gccacccatc
      781 aggatggaaa tagcggctat tgacatttat aacgttacgg aagccgtttt ttcttctgtc
      841 tcaatttctt tatttttgcg ttggttaatt tcctcaataa tcagtgcaaa ccgtttctca
      901 ttgagcgtgt aaaagaaccc catcgtcaat gcagcaataa ttgccagcgc acaaggccag
      961 ataaaaatga gctgacgcaa accctgtaat gttgcatcgc tttgcgcgat gttgggaaca
     1021 taaccgattt gcgtcagcat aatgcctggc aagaagccag caagtgcggc tgaaatttta
     1081 cgaaagaagg tataaccggt ataaacagaa ccttcggcac gtatacctgt tttccattca
     1141 ccataatcta cggtatctgg taccagtgcc cagttcaggc tgtttacaaa ggccgtgcca
     1201 aaaaaggcga cacaagagaa catgacgaaa gtgaatgagt tactgcccca gcaataattc
     1261 aggatatccc ccaccgccca cagcaccatg ccagccagat aaacctgttt tttaccaaaa
     1321 cattttacag ttagtggcac cagtaatacg ccgacgagaa tgcatcccat actaaagaat
     1381 cccatccacg acaataagtt aatatcattc agcacatact gggtgtaata gacctgaatc
     1441 gccagtttga tattaaatgc cgccagggta cacaggttag caatgcacaa taccagcaaa
     1501 ggtggattac taaatattgc gtaaaatgat ttgagaatac tggccttatg tccggttggc
     1561 gtcgcgtcca cataatgctc tttgacacct ctgtagcaca gcatcataaa aataaagccg
     1621 ccaatggaga acatcagtgc cgcgcaggcg tagccgacgg tggagtcaga aaatagtgac
     1681 tgcaaaggaa taaacgccac ggtacagatc aatagcccta tggttgcacc accctggcgg
     1741 taagcagcga gttgcgcgcg ctcattcggg tttttggtaa tggccgggat catcgcgcca
     1801 tacgagcagt tcatcagact gtatgaaagt ccaaacatca taaacagcgc ggtggcaatc
     1861 gtcgttttaa ccggcaaact aaaagtggtg gcgataaact ggagcgttgc aattaacgcc
     1921 gccggaatcg ctgcgtataa aataaacggc ctgaattttc ctttaggtcc aatatttttc
     1981 cgcgagtcga gtaaaaatcc ggtgagcata tcggtaaatg cggtaaagaa tttcgccacc
     2041 agaaagataa tcccgccata gtaagcaggc atccctaatt catcggtata aaatttgagg
     2101 agatacagag tgccgatgct aagcattaaa ttcgaaccga cgtcgcccat accataggcg
     2161 attttttccc gcagattcag ttttaatgtc agtggattat ggttagacat aatcactcct
     2221 tataacgccc cgcagggcgt tgtttattat aattacgccg tgcgtttacg ggcttctatc
     2281 tcttcaacaa tgcggacgta catcttctcg ttcagactgt agaagcaacc cattgccaca
     2341 atggtgacta ctgccagtgc gcttgggtag atgaagatca gttggcgcaa cccttcgata
     2401 gtgtggtcag cctgggcgac gtttggcaca tagccaattt gcgtcagcat ccagcctggg
     2461 aagaaacctg ccagcgcctg agatactttg cgaaagaagg tgaaaccggt gtagaccgta
     2521 ccttccgaac gcacaccggt gcgccactcg ccatactcga cggtgtcaga aaccagcgcc
     2581 cagttcaggc tgttaacaaa cgccgaacca aagaacgcca ggcaggagaa agccacaaag
     2641 ctgaccgagc cgccgccgaa gaaatagttg agcagatcgc ccagcaccca gatcagcaga
     2701 ccgccgatat agaccttctt cttaccaaaa cgtctgactg caccaggcat caggaacacg
     2761 ccgatgaaaa tacagcccat gctgaaaaat cccatatatg acaacaggat gggatcgtta
     2821 agcacgtact gggtgtaata gacctgaatg gcgagcttga cgttaaacgc ccctaaggta
     2881 cagaggttgg caatgcacaa aatgaacagt gggcggttcc ccgcaattgc gcggaaagat
     2941 tgcaacaagc caggcttttg cgccggattg gtaggttggg tttcgacata acgctctttc
     3001 acgcccgaat agcagatcca cataaacagc aggccgaaca gtgaaaacag cgtggcggcg
     3061 aagatatagc caagttgctg attaccctcg ataagattca taaccggcac gaatcccacc
     3121 gtacacagca gcaggcccaa tgtagcgcca ccctgacgcc atgctgccag cgatgcacgt
     3181 tcgttggggt ttttagtaat agcggggacc atcgcgccat acgagcagtt catcatgctg
     3241 aaaaacagtc cgtaaagcat aaacaggatg gtggccatca ccgttttacc ggtgacatca
     3301 aacggtgtgc cgacaaagtt ggcgatcgcc agcagggtga ccggaaatga cgcatacaga
     3361 ataaaaggac ggaacttacc ttttggaccg atcttgcgtc gggaatccaa cataatgccg
     3421 gtacccatat cggtaaaggc ggtaaagaat tttgaaatca aaaagataat gccgccatag
     3481 gtgccaggca gccccagaac atcggtataa aacttcaaaa gataaagcgt gccgatatcc
     3541 agtaggatgt tagaggccag atcgccgata ccgtaagaga gtttctcttt aaagggcagg
     3601 cgtaaagtag ccggatcttc cgttgtgatg tgactcatcg ttgttctccc tttgcccgtg
     3661 agtttcccca cgggcggatg tgattagatg ctttttaatg acgcgaacag tgccgcccat
     3721 tcgctatcgg cgcgataaaa gaccggcggt ttaccgatgg gcgcattaac ggtaacttcc
     3781 ccgccccgga acgcttcacc cgtccaggca tggacccagt tatcctccgg cagatagagc
     3841 gtccagtcgc tgcgaccttc ttcgtgtacc ggggcgacca gaatgtcgcg gcctaacagg
     3901 tactgatatt tcagggtgta ggtctgtgca tcgtcttcgt aatgcaggaa cagagggcgc
     3961 ataaccggca ggccggactt cgcattcagt gcgacggcct ctttcaggta aggtttcagg
     4021 gtagtgaaga cggtggtcat acgggcaaaa tgggcgatag tttccgcgtc gccgtcaaac
     4081 tgccagttgt cgccaggacg gttaccttca tgggtgcgca tcattggcgt gaaggcgctg
     4141 aaatcgcacc agcgcagcag cagctctttg ctgcgcttca tctcaaacag ggtggtgtaa
     4201 ccgccaatat cactgtgatg caggccatgt ccggtcattg ccagcgacag cgccgccggg
     4261 acgacagacg ccagtccatc gtccagactc cagtcaacgt tctggtcgcc cgcccacatc
     4321 atggtggagt atttctggct accggtggaa ccggcgcgca taaagaagag gatctcgccg
     4381 agcttgcccg tttcttcaag ggcttcgtag ttacatttcg cccacagcgc aggccaggcg
     4441 ttatgcataa tttcggcgct gacgccgtta tgcaggtaag tgtcggtggg cagatactcg
     4501 ccgaagtcag ccatccagcc gccgcagccg agttcaatca tgttcttttt gatcacttcc
     4561 ttgaaccagg cgtaggcttc cggattagtg agatcgacaa cgccgccgta gaactcgcca
     4621 aactccacca gataatcgcc gccggcggca tcttttgcca gatagccgcg ctttgccgct
     4681 tcttcgcaga ggtctttatc gctggcaaca tacgggttga tataggcaag gaactgtacg
     4741 ccctctttgt tccactgttt gatacgtgaa tccagttgcg ggtagttttc gctgttccac
     4801 ttccagttcc acattacgcg tttgccaaaa gaggtcatac gaataccgga ccaatcctgc
     4861 gcccagatgc cgttgacctt cacgcccgcg ttacgcatgg tgtccagttt cttctggcac
     4921 acctcagtgc cgccctgaat gccgagcgtt actccgtcat aaatccagtc gggcagttct
     4981 gtctggcgtc ccagcagcgc ggttaatttt tccagcaggg agatgtatgt atcagcacat
     5041 tcaaaacgca gtgttgcttt gtcttcccac agcgccagtt catggtattc cggcgcgcta
     5101 aagtcgaagt tcatatagca actgttatca acatggcagt aatacttctg cgtgctaaca
     5161 aacgtgggtt gtggaaagaa ggtccagtag taatcccctc ccgcattctc tttgcagtcg
     5221 gcctgccagg tgacataggt ttgtttgttg cgaccaacgc cttgttcact ggtccacagc
     5281 gggaacggtt tgccgcgcag atcaaagtag gagaattgtt cgccgcagcc gtagatatga
     5341 tcttccggct gtgcggcaag gcgcagccag atgcggttgt ggttaaggtt gtcgttttgt
     5401 agttccagca ataaacggcc atgatcgtcg gcagagatat tcagcgtggc gctaatgtca
     5461 gaaccacggc tgaaatggat taaccagcca tccggtgact ggctgacgat ggcgtcggta
     5521 agcgcaattt tctcctgcaa tttatcttta atgctgaaat taccgcggaa catatcgata
     5581 tccgctatac ctgaaccaat ccataaacaa ggattatctt tgctatggat taaaataagg
     5641 cggtcttgaa aacgtagagt aaaactgtcg ttattctgat gaaattgaaa atctaatagc
     5701 tgtgggcgtg gcgtatccat aaataatctc catagatata aaactaatat ttcatgccat
     5761 cctgttatta caggtttcat taattaaaga atgtgtgcgg gtataaatta aaacccgcat
     5821 aatgattatt tactttcttc cgcccgacgg attgccgcca cttttttacc aaatgccagt
     5881 tgcagtataa ctaacaaggt aaacggagga agaagtgcgg cagcggcggg aattcccagc
     5941 gcgcctttta tcaacataga accggaggca atcagcaata aaaagaccat cacacggaac
     6001 actttgaccg aaatgcgttt atgcacatat tgtccaacca gtgaaccgag gatcatggtg
     6061 ggaatacaaa acagcaccag tttaaagata gagacagtca aaattccgct ggtcgccagc
     6121 ccgccgatca ccgcgatgtt gtttgcggtg aagaaagcgt tcagagtgcc gcgaaatgcc
     6181 gccgggccta agttacgcag cataccgtag ataaccatcg gcggtccgtt ggttgaaaat
     6241 gctgccccta acgctcctgc cacggcgccg ataggtgcgg cgatccagct acggtcataa
     6301 atcggcaggc gtggaataaa catactgtaa aaggagtaca ggataaggaa tccgcctaag
     6361 cccactttca taaaatggtc gggtaaatac gacagcgcat ataaaccgac gggtattccc
     6421 agaaacgaga aaataatcag taccattgcc gatcgccaat ccgtttcttt acgtgacagc
     6481 caggtggcgt acaaggcggt cgcggtgccg acaatcaccg agagcggagt tgccattttt
     6541 acggggagca ttaacgtgac gagcggcatg gtggttaaac caccgccaaa tcccgcgcat
     6601 atccccacaa aggtataaag aaacattaac gcgataacga tacataccac gtttgtttcc
     6661 agcatgaccc catccggtga aaataacgta aaaaagtcca taatatttcc tgttgtgtaa
     6721 atacaactct aaacaatgat ctcgttcttt tcttaataaa attaattgtg ctgcccgtga
     6781 atattaaaaa acaggcggtg cgttttccct ggttccagta ataataagtc gatacctgaa
     6841 ttaaaggcgt taggcgggca actcattggt tcaactgcca gtccctgacg ttgtaatttt
     6901 tcaccgctat aaacctgcac ccagggttga tcggaacaga ggctcaccga aagcgcctgc
     6961 tgtggatggg taatagtcat ttcccacaaa tcattagcgg ttttaaaggt gtgatcgatt
     7021 tttgtcgcgg ctattttttt cgcctgcgtg aaatttaaat ccagctcatc aacgtggtga
     7081 agcgttgtcg gattagcgtg ctcatcgatg gcataaactt ggtttgccgg aagctggaac
     7141 agatattcat caacagatgt taagttgcag gttaaataag ggtgaatacc cacaccgtaa
     7201 ggcgctgcga cagtgccgat attctggctt gcgatctcaa cggaaagacc cgtgcgggcg
     7261 ttgagcgaat aaaccacctg cgaagccagc ataaacgggt agccgtaact gggtggcaga
     7321 aaggcggtta atgtgacgct ggtggcgctt aattcgctga tttgccagtc acgccaggcg
     7381 agcagcccat gaatagcggc ttttgaacca tgttcattaa tgggtaactg atattcctgc
     7441 ccctgatagc gataacagcc gttggcgatc cggtttggcc acggaataag gactttgccc
     7501 agatgtgcca gcggcatctc ttcgggttta tgcggaataa ctaaatgaca gccctgaaag
     7561 gttaattcag ccagacctgc accgacggtc acaatggtcg cgtgataatc ccccgcggca
     7621 agtgaaacgg tctgtccact gcaatgcatg ttggtgattt gcatacgagg tacctttatt
     7681 gaaaaacgac atacccggcg tgcagcctga tatgtcgttt ttttagtcag ttatttcgcg
     7741 ttaatatcca gcagacccgc cgcaaccgcc ggagccaggc ctggcgctaa cgggatacgt
     7801 gggatcacca gacaatgcag cagatgataa atatcctgtt tgccgtccca gactttggtg
     7861 gtgaccttat tgtccgcatc cagctcctgc caccatgaac cattttcgta gtccatcagg
     7921 tatttgatgc agtagtccca ccatgtctga taccaagttt cgtactggcg atcgccagtg
     7981 acggtgtaga gcgcgtaggc cgtacccatc gcttcgacga taggccaacg cacacgttcg
     8041 cggaccaccg gttttccttc ccagtcaacg gtataaacaa tcccgtccgc gccatcgggt
     8101 gcccaggcat cgcgcacggt ggcgttaaac agacctttgg catcttctag cagccatgct
     8161 ggcggttgtt cgcaacgggc ttccagggcc gcgtgaatgt gcagcattaa acggccccat
     8221 tcgatccagt ggcccggagt acctccgaac gcgcggaagc ggtgcgccgg gttatctttg
     8281 ttgtaatctg gcagcggatt ccactgggta tcgaagtgtt cgttaacgcg ataatgatta
     8341 tttctggcga cgtcgtggat aatcacggaa gccacgcgaa tcgcgcgatc cagccatttt
     8401 ttgtcatggg tgacgtcata gacaatcagg aaggcttcca ccgcgtgcat attggcattg
     8461 ccgccgcgat actcttcggt tttgctgaag gcttcatccc aggactccag gcacatctgc
     8521 tcttcttcgc tccagaagta tttctcgata atttcaatgg tgtaatcgag cagcttgcgc
     8581 gcttccggat gaccggtagt gacggcgctg gcagcaccca gcagagcaaa gaaatgttga
     8641 tagccctgtt tggaggcatc caccacgccc tcgtcattca cgcaggcata ccaaccgcca
     8701 tattttttat cgcgcagtgc gccattcatg gctttgatac cgtgatcaac caacgagtaa
     8761 gcgccaggtc ggcccatcgc cgcagcaacg gaataaacgt gcaacatacg agctgtgatc
     8821 cacagatggg tgcccatctc ttctttgatt tgccctttat tgcctaacca gccaaatccg
     8881 gtcggtacta cggatttttt accaaaatca aagatgcgat cggtttcctg ttccagccaa
     8941 cggttgtgac ttaaagtgtt aaaccatttc atttttgtat ccccatctta acggcgttta
     9001 gccatcattt cgtcgacgat atcgccaagt tgttgtaatt tcggtgcgca aacgtcacgc
     9061 agcatcagct cgttgtctgg taaaccgacg accgatgccc agacggcacg accagccagg
     9121 aagcccgatg cacccgctgt cattgccacg cgtacggcac gcgggaacag tttttcgtcg
     9181 acaccggaag agaggatcac ccacggcata ttgatatggt cattcagacg ttgtgaagca
     9241 ctgagaagct cttgttgcgg acctttaccg taaagcggca tttcaacttt gtagagatca
     9301 gcgccactgt cgcccagctc tttggctgca tcgatgattg cttgttcgcg atcgaattta
     9361 tcgccacgac gcggtgggcg gacgactggt tcaatgatgc ttaccagacc gtgtgagtgg
     9421 cacatttcgt taaactcttt caccatatcc aggcgttgct gcgcatcttc atcactacgc
     9481 cacagcacca gcagttttaa ggctttgcca ccgtcttgtt tgatttgtag cggattgatt
     9541 ttgcggtcaa tgaccacgct atctaccgga ataccgttgc caggaatgaa ctcatcggcg
     9601 gcgacaatca tggcgcaact tttggcaatc gcgttttgct caaccacctg gcggtagcag
     9661 aattgttgat ctaccagaat cgccgatgca taaggtgaaa gagtcttcgc ggcgttaact
     9721 ttgaaatcag ttaaaacgct atcggctact ggagcgggtg cgccagccgc ggcaaacatc
     9781 atgcgcatgg cttcgcgctg atcgaccgcc agcatggcaa aaccgcccga tgcgcgcgta
     9841 atgtcgttga tggtgtactt attcattcat ttattccttt atccagtcgt tacattttta
     9901 ctttggcagt catcccggca ctgacacgga cctgctccag aatggcggac cagtcctggc
     9961 gaccgcgacc cgctgcgcgc gcctggctat aaacctcccg tgaggcggcc cccagtggca
    10021 tcggcacatg caactggttg gcgacatcaa gggcgatgcc aagatcctta tgggcaagat
    10081 cgatcatgaa ggcgggagaa agatccccgc tgaggacttt gttcggccag gaagtggtga
    10141 agtggccttt accggcggcg gtaccgctca tcaccttgac ggcaacatcg aagggaagat
    10201 tcagggcttc gcacaaaacg gcggcttctg ccgacaacgc attgagcgcg atgctcatgt
    10261 agttgttgat aagcttaacg cggatcccca tgcccggacc gcccgcgttg atcaactcgc
    10321 tgcccatcgc catcaggatc ggcgtggcgc gttcaacttg ttcagcagtg ccgccagcca
    10381 gcagtagcag agtaccggta atggcatttg cagaagtacg gcctaccgga acatccatca
    10441 tgctgaagcc tctggcctgc atatcagcaa tcaatttatc ggtttgcagc ggatggatgg
    10501 tcgacatatc aatgaccagc gcatcggaag ataagctctc acagacaccg ttttcaccga
    10561 acaacacgct gcgcaccaga tcaccattcg gcaacatggt aatgataaat tcggcatctt
    10621 tagcggcctg cgccgggttg gcggcgggag tcgcaccttt gtctaccaga tgccgcacag
    10681 cttcggcgtt cacatcaaag acgcgaagtt gatgcccttg ctgcaataaa ttgctcgcca
    10741 ttggcgaacc catttgccct aaaccgataa acgcgattgc tgccataacc ctctcctgaa
    10801 tacagtaatg tcactttttg tcatttatga catgccttgc ttgtctgttt ttgatcgtat
    10861 ttgtaattta tcgtcaaaaa attgacagcc gtcacttttt aaacaattgg tgaaattaaa
    10921 atgaacgcat cccaaaatgc ttaaggaatg accatgattc gtgttgcttg tgtaggaatt
    10981 accgtgatgg atcgcatcta ttacgtggaa ggattgccga cggagagcgg taaatacgtg
    11041 gcgaaaaatt atacggaagt tggcggcggg ccagcggcta cagcagcagt tgcggcggcc
    11101 agactaggtg cgcaggtcga ttttattggt cgcgtcggtg acgacgacac aggcaacagc
    11161 ctgctggcag agctggaatc ctggggcgtt aacacccgtt ataccaaacg gtataacgag
    11221 gcgaaatctt cgcaatccgc catcatggtg gataccaaag gcgagcggat aatcattaac
    11281 taccccagcc cggacttgct gcctgacgca gagtggttgg aggaaattga tttctctcag
    11341 tgggatgttg tgctggcgga tgtacgctgg cacgaaggtg ctaaaaaagc cttcaccctg
    11401 gcccggcagg cgggcgtgat gactgttttg gacggggaca ttacgccgca ggatatcagt
    11461 gagctggtgg cattaagcga tcatgcggcc ttttcagaac cgggtctggc gcgcttaacg
    11521 ggggtgaaag agatggccag tgcgctaaaa caggcacaaa cgctcacaaa tggtcatgtc
    11581 tatgtgaccc agggcagcgc aggctgcgac tggctggaaa atggcgggcg tcaacatcaa
    11641 ccggccttca aagttgatgt ggtagatacc acaggagcgg gtgatgtttt tcacggcgct
    11701 ttggcggtgg cgctggccac aggtggggat ttagcggagt cagtccgctt cgccagcggt
    11761 gtagcggcgt taaaatgcac acgtcccggt ggacgagctg ggatccctga ctgtgatcaa
    11821 acccgatctt ttttgtcact ttttgtataa aatgccaggg tgatggtttt tcgaggaatt
    11881 ttcatgagcc ttaccgaact aaccggtaac ccgcggcacg accaactcct catgctgatc
    11941 gccgagcgtg ggtatatgaa tattgatgag ctggcaaatc tgctggatgt ctccacgcag
    12001 acggtccgcc gggatattcg taaattaagc gagcaaggac tgattacgcg ccatcacggt
    12061 ggcgcgggtc gggcttccag cgtcgttaat acggcgttcg agcagcgtga ggtttcgcaa
    12121 accgaagaaa aaaaagcgat tgccgaagcg gtggcagatt atattcctga tggttcaaca
    12181 atatttatca ccattggtac gaccgttgag catgttgccc gggcgttact taaccataac
    12241 catttgcgga taatcaccaa cagcctgcgt gtggcgcata ttctttacca caacccgcgc
    12301 tttgaagtga tggtgcctgg cggaacgctg cgttcccata acagtgggat cattggccct
    12361 tcagcggcgt cctttgtggc cgattttcgc gccgattatc tggtaacaag tgtaggcgca
    12421 attgagagcg atggcgcgtt gatggagttt gatgttaacg aagctaacgt ggtgaaaacg
    12481 atgatggcgc acgcgagaaa tattctgctg gtcgccgatc acactaagta tcatgcttcg
    12541 gcggcggttg aaattggtaa tgtggcacag gtcactgcgc tctttaccga cgagctgccg
    12601 cccgctgcgc taaaatcacg cttacaagac agccaaattg aaatcatcct tccccaggaa
    12661 gacgcgtaga attttgtgac cgttgacctg gcttcatacc tgaccacata gccaaaccca
    12721 tcctttcccg ctacagttaa tttcttgtgg cgcgaaagga ggcaaaaatg ctctatatct
    12781 ttgatttagg taatgtgatt gtcgatatcg actttaaccg tgtgctggga gcctggagcg
    12841 atttaacgcg tgttccactg gctacgctta agaagagttt ccacatgggc gaggcgtttc
    12901 atcagcatga gcgtggggaa attagcgacg aagcgttcgc agaggcgctg tgtcatgaga
    12961 tggctctacc gctaagctac gagcagttct ctcacggctg gcaggcggtg tttgtcgcgc
    13021 tgcgcccgga agtgatcgcc atcatgcata aactgcgtga gcaggggcat cgcgtggtgg
    13081 tgctttccaa tactaaccgc ctgcatacta ccttctggcc ggaggaatac ccggaaatcc
    13141 gtgatgctgc tgaccatatc tatctgtcgc aagacttggg tatgcgcaaa cctgaagcac
    13201 gaatttatca gcatgttttg caggcagaag gtttttcacc agacgatacg gtctttttcg
    13261 acgataacgc cgataatata gaaggagcta atcagctagg cattactagt attctggtga
    13321 aagataaaac caccatcccg gactatttcg cgaaggtgtt atgctaaaaa ccattcagga
    13381 caaagccagg catcgtaccc gtccactatg ggcctggcta aaactactct ggcaacgcat
    13441 tgatgaggac aacatgacaa ccctggcagg taaccttgcc tatgtgtcgt tgctctcatt
    13501 agtgccgctg gttgccgttg tttttgcgct tttcgccgct tttcccatgt tttccgacgt
    13561 cagcattcag ttgcgtcact ttatttttgc caactttctg cctgctactg gcgatgttat
    13621 ccagcgatat atcgaacagt ttgtcgccaa ttccaacaaa atgaccgccg tcggggcgtg
    13681 cgggctgatc gtcacggcgt tattgttgat gtactccatc gatagcgcgt tgaataccat
    13741 ctggcgcagt aaacgagcgc gacccaaaat ttactcgttt gccgtgtact ggatgatttt
    13801 aacgctgggg ccgctgttgg caggggccag tctggcgatc agctcctatc tgctctctct
    13861 gcgctgggcg agcgatctca atactgtcat cgataatgtg ttgcgtattt ttccgctgct
    13921 gttgtcgtgg atctctttct ggctgctgta cagcatcgtt cctaccatcc gtgtacctaa
    13981 ccgcgacgcg attgtcggcg cgtttgtcgc cgcgctcctg ttcgaagcag gaaagaaagg
    14041 tttcgcgctt tatatcacca tgttcccatc atatcagctc atttacggtg tgctggcggt
    14101 gatccccatt ctctttgttt gggtctactg gacatggtgt atcgtcttgc ttggcgcgga
    14161 aattactgtc actctcgggg aataccgcaa attaaaacaa gctgctgaac aagaagaaga
    14221 cgacgaacca tgattgcatt aattcaacgc gtaacccgtg ccagcgtcac cgtggaggga
    14281 gaagtgacgg gcgaaattgg cgcgggactt ttggtgttat tgggtgtcga aaaggatgac
    14341 gacgaacaga aagcaaaccg tctgtgcgag cgtgtgcttg gctatcgcat cttcagcgat
    14401 gccgatggca agatgaatct caacgtgcaa caggcgggcg gcagtgtgct ggtggtttcc
    14461 cagtttaccc tcgccgcaga taccgaacgg gggatgcgcc caagcttctc aaaaggcgca
    14521 tcaccggatc gcgcagaggc gttatatgac tatttcgtcg aacgctgccg tcagcaggag
    14581 atgaacacgc aaacagggcg cttcgctgcg gatatgcagg tatcgctggt caatgatggc
    14641 cccgtgacat tctggttgca ggtatgagcc agcttccagg tgtgtcacgg gaaacaagag
    14701 agagtatcgc tatgtatcac cttcgggttc cacaaacaga agaagaatta gagcgctact
    14761 atcagttccg ctgggagatg ttgcgtaaac ccctgcatca accgaaaggt tcggaacgcg
    14821 acgcgtggga tgcgatggcg catcaccaga tggtcgtcga cgagcagggt aatctggtgg
    14881 cagtagggcg gctgtatatt aatgccgata acgaagcgtc catccgtttt atggccgttc
    14941 atcccgacgt gcaggacaaa gggttaggca cgctgatggc gatgactctg gagtcggtgg
    15001 cgcgtcagga aggcgttaag cgcgtgacct gtagcgcccg tgaagacgcg gtggagtttt
    15061 tcgccaagct ggggtttgtt aatcagggag aaatcaccac gccaaccacc acgccgattc
    15121 gccatttttt gatgattaaa cctgtcgcca ctctggatga tattttgcat cgcggcgact
    15181 ggtgcgcgca gctgcaacag gcgtggtacg aacatatccc gctaagcgaa aaaatgggcg
    15241 tgcgcattca gcaatatacc gggcaaaaat ttatcaccac catgccggaa acaggcaatc
    15301 agaatccgca tcacacgctg tttgccggga gtttattctc gctggcaacg ctcactggtt
    15361 gggggcttat ctggctgatg ctgcgcgaac gccacctcgg cggaacgatt attctggcgg
    15421 atgcgcatat ccgctatagc aaaccgatta gcggaaaacc ccatgcggta gccgaccttg
    15481 gtgccttaag tggcgatctc gaccgtctgg cgcgcggacg aaaagcacgg gtgcagatgc
    15541 aagtagaaat ctttggcgac gagacgccgg gtgcagtgtt tgaaggcacg tatatcgttc
    15601 tgcccgcgaa gccatttggt ccgtatgaag agggcggaaa cgaagaagag taggggcgtt
    15661 ttacttgact gaaaaggttt tcaatctgaa aaccttttct gggccctggt ccggagttac
    15721 cttcccgctc tccaggcaac atcccgtagc gccgatcgcg cagcctcact gataccacct
    15781 aactgaaaaa atccgtggat aacgccaagg tagcgttggc aggtgcattg cactccttgc
    15841 cctgtcatgc ggtgatacaa cgcttcacct tcatcgcaaa gtgggtcgta ttcagcggta
    15901 atgatatgta caggtggcaa gccattgaaa tcattgcgcc aaatagggct ggcttcagga
    15961 tgcctgcgtt caatgcctgg caggtacatc tcaaagccgc tcagtaaagt gtcgcgtgtg
    16021 atgatgtaat cgtgaccatt gcgtatgtag ctttcgaaat gcgcagtcgc atcaagcata
    16081 ggataaatta agatgagttg tgctggttgc cactcgccag cacgcttcaa tcgcaaagca
    16141 gttaccagcg ccagatgccc tcccgcactg tcaccggcaa gagttattcg ttgtttatca
    16201 acgccgagtc gttcggcgtg ttgccggact aattctgcac ctcgttgtgc atcatcatgg
    16261 gcggcgggga aagtatgttc cggtgccagc cgatactgga cggcaataat ccgacattgc
    16321 ccgtaatagg ctaactggcg cagctggttg tcgtgagttg cgaacccgcc gctaacaaaa
    16381 cagccaccgt agtaataaat tatcgtcggg agtaatgtcg gggcattcag cggtgacact
    16441 attcgtaagg tcatcccttc aagttcgata gtctgaatat ctacccgcgt ttctgtctca
    16501 cccgcaagga cagcgctggc gatatagccc tctctgcgct gcgtaatact ttgttggcgc
    16561 gatgaggggc gacccgcagc gataaactct tcgactaact ctgcaattcc tttttccagt
    16621 gccatagtag aactcatttt gctgtatgaa tgtacagttt tatacacccg tttttgctgg
    16681 atggatacag ggaaattcac gggcgtgaaa aatctggaaa gcacctcgca aatgtaaaga
    16741 caaacccggc agagacgtgg ttttgctgga gcactatcag taagctagtg gtttcaatgg
    16801 tacgtcaatg acgtacaaaa aggattgtta ctctaagcca atggtgtata attatgctct
    16861 tcatcgaaac ggaaattttt actgaagatg ttcaaaaact gctgaacgac gatgaattca
    16921 gtcgttttca gtttttcctg gccttgaatc ctgactatgg tgaggtaata cctgaaactg
    16981 gtgggttaag aaaggttcga tgggtctcag gaggaaaggg gaaacgtgct ggtgtcaggg
    17041 ttatttactt tcatcaggtt aaacattatg agatcagatt actgcttatt tatcgtaagg
    17101 gtattaaaga tgatctgtct ccccaggaaa aagcaatgct ccggttgtta aatacgaggt
    17161 ggtaaatgga taaggcactt tttgagcggt tgactcacag catggcccag atgaatgaaa
    17221 ttattgaagg aacgcgacag ccttcacgta cttttgaagt tgatgctatg aaaatcaaag
    17281 aaatacgccg cgcttcaggg ttgtcacaat ctaaatttgc cgatcttatt tccgtaagcg
    17341 tagacacgtt gcgtaactgg gagcaggggc gacgttcacc gaccgggcct gcaaaggctt
    17401 tgctgcgagc gattgccaat gatccgcaac atgtgctgca agcacttaac cgttagaggc
    17461 atggttatgc catacagaaa gcaccagcat tcagaactcc tatgaaagcg gcatagtgcc
    17521 gcctgctcag gagttttgct atgacccgta atcgtgctga acgtcgccat catatggtgc
    17581 gattaaaaaa aatccgctgt cgttatcgca ctgcaggcga tggaagcaaa aaagcatcag
    17641 gtatttgctt tcgtactccc tgtatttgtt cttgttggat gtgtgggcat cgacgctatc
    17701 ataatggtcc ccgcgtatcg gaaattcgtg caaaagcacg ctatgcgaat taagggaaaa
    17761 tgatgaacta ttttaaggaa gcacattttt agaactctta acaggagact aaaaatgtcc
    17821 acgtttaata cgcgtaaaga tagggatcgt aatggtaaag cgaagccgca taagcattca
    17881 gggatcccaa cacttacagg taaatggcgt cacccttcaa tggattgttg ggtaaactac
    17941 agtacaccgg gcgatcatga atggaagcgg ctctatacta cgcttcgccg tcgcgcagat
    18001 gatcgacgcc aatgtcatcg ggtaatgcag ggggatgatc cggaaggaat cgtctggttc
    18061 ccggactgtt atccacaaat ttactactac taacaattaa agaccggttg gtgcggcact
    18121 gaccggtcgt tatgaatggt agatttcacc ttaaaccctg gtgcggtaaa gctgctgact
    18181 ttttcgggta caccatcctg atgaagcaaa tgaagaccca atcaaaaaat attcttgaat
    18241 ataaaatatt tatagcatcc ataattcatc cttattattt gttgtttctt ttctgtagat
    18301 gtgcccccgc ttaattaaaa gtaataaacc atttttgagt aacaattgaa ataactgaaa
    18361 ctgttacatt atctttggat gccgtctcgc aaaaacaaaa caatatgaaa aaatccgctt
    18421 tcgttaatta actttccggc gtaattttcg cacatacatt aatgagtaat atatgtaagg
    18481 tggtggtaat ggcgatgaat acggtttttc ttcatttatc agaagaggca attaaacggc
    18541 tgaacaagct tcggggatgg cgtaaggttt cgcgctctgc aattttacgc gaagcggtag
    18601 agcaatatct ggagcggcag caatttccgg tgcgtaaggc aaaaggcggc aggcaaaggg
    18661 acgaggcagt tggtgttgaa gaactatgta agcagcataa ggaatgacaa aagttttttt
    18721 attcatgaat ataaaaaacc agatgccctt attctggtat taatacaagg ctgttttact
    18781 tgaacttata ataactgcaa ctgttacatc atatctggaa aacgcctcgc gaaatacgag
    18841 agattttgcg ggaatagcag gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc
    18901 gccacccgga aaacgtcaga gattactccc cttcacccgg aaacaggaac gggttaatgg
    18961 aactacgggc atagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat
    19021 ctgccacggc ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccgc
    19081 agtcatcgca gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca
    19141 gtttgccgct ttgttcacag ttgctgcatt ttacgcgcac tacgtgccat tcagtttcac
    19201 acaggttgca gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca
    19261 ccggcataga gccacatacc gggcaatatt gacgttgttc gccgtattca gcgcgggctt
    19321 tgccggggat cagattagcc atctgcgccc agtagagcga cagcgcagcc cagataaacg
    19381 gcgctttatc gctgctgacg gacgagaaat cagaggcaaa cagtgcgctg gccatatctt
    19441 ccagctcctg agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca
    19501 tttcaggttt cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg
    19561 gcagaacgtg aatatccagc gggggcttgc cttgtgcgct ggcttcttta atgcgcgtgg
    19621 tcagatccat ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg
    19681 cagcaaagcg caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg
    19741 cgcggcggtt gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg
    19801 tacgtttctc gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt
    19861 cttttccgtt gtcttgcgga cctcacggta ccagcgcggg tgatgtttct ttgcccatgc
    19921 gctggtcacc catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta
    19981 gatatgcacc atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc
    20041 gaatcggatc accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc
    20101 gctcaccagc aacaggacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa
    20161 gttataacgc ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc
    20221 ccaaaagata tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat
    20281 gaacgaggca aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt
    20341 gcccatgatt tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc
    20401 cgccaggatg aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg
    20461 cacgatggtg tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc
    20521 tcttcctcat ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggctgcgaag
    20581 gtagccgcaa agccaacagc tgcgagcggt ttccagatgc ctttccagaa tttcacggtt
    20641 tcgctgattt ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc
    20701 agcacgtaca taacatgtgt accaccgacg cctgccgggt cgtacaaacc cgcattgtcg
    20761 taaccacggg ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat cgactctttc
    20821 gtaccgaagt gaatcgcgcc cgttgggcag gtcttcacgc aggccggttc ttgcccaacc
    20881 accacgcggt caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc
    20941 ggaatgtcga acggacagcc cgcaatgcaa taaccgcagc cgatgcactg ctcggactgg
    21001 aagtcgacga taccgttggc atactgaata attgcccctt ccgccgggca cgctttcagg
    21061 cagcctggat cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg
    21121 ttctgctcca cttccgagaa tcgcatcacc gtccacgatt tggcacttaa atcattgggg
    21181 ttgtcgtaca ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac
    21241 gccacctgac aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct
    21301 tcctggaagt cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg
    21361 atatcttgcg attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg
    21421 aaggacttaa actccggcgt ctgcgtgttc gcatcaccga cgaatggcgt caacgtattg
    21481 gcaataaagc cttttttcgc aacaccttca tagccccagt gaatcggaat accgatggta
    21541 tcgatatctt tgccgtttgc tttcagcgtg cgaatacgtt tggtcactac cgctttggct
    21601 ttgatatagc cacggttgga ggagactttc acagtatcgc cctgggcaat gccaagttta
    21661 ttcgccagcg actccccgat ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc
    21721 gcgtgtttgg tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg
    21781 aacttatcgg ctttacctaa tgcttcggca tcgtctttaa agatacgcgc cgccggattc
    21841 gagataacgt ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt
    21901 tccgggaacg gaccttccgc catcttatcg agggcaaaca gacgtcccat gccttcctgc
    21961 tgcatgataa acggccctac gccgctgccc ggaggcgctg cgctgtaatc cggaatatcc
    22021 cagccggtcc acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta
    22081 ccctgcggat ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat
    22141 gcccagccca gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg
    22201 ttgccttccg gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtgccgtca
    22261 tcgcgcagtt gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt
    22321 gccgggtcgg taatatcggc cagcgctttg ccgttgctct ccattgccac ttcttctgaa
    22381 gatggctcat gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg
    22441 ccaccctgtt cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca
    22501 tcagtcagcg caatccccgg ggcgtccgca cctttccagt gccactgtaa ccagcgacct
    22561 gagttaacga tagaaccgtt ctcttccgcg aagcaggtcg atggtagacg gaacacttcg
    22621 gtctggatct tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag
    22681 gtttcagtgt tcagcgggtc gatagtcacg aggaatttca gcttcgacag acagccgatc
    22741 actttgtttt tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg
    22801 accttgccct ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc
    22861 ggcaaccagt caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc
    22921 atcatagaga cgaagaattt cgggtagttg ccccagtagt tgacctggcc ttccagcagt
    22981 ggttttggcg tgttggcagc gaggtaagtt tgcagatcgg tctgcttctc gcttggcaac
    23041 gtcatgtaac ctggcagact ctgcgacagc agccccaggt cggtcagccc ctgaatattg
    23101 gagtgaccgc gcagggcgtt aacgccgccg cctgccatcc ccatgttgcc gagcagcagc
    23161 tggatcatcg ccatcgtacg aatgttttgc gcaccaatgg agtgttgcgt ccagccgagc
    23221 gcgtaaagga acgaggcagt tttatcgtga gcactggtct ctgcgatgta ttcgcagact
    23281 ttcaggaacg cgtctttcgg cgtaccacag atgttttcaa ccacatctgg cgtataacgg
    23341 gaaacgtgct gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt
    23401 ttggcgaagc cgttttcgtc cagctcgtag gtccagctgg atttatcgta cttgcgtttt
    23461 tccgcgtcgt agccggtgaa caggccatct tcaaagccgt aatcctcacg cacgatcagg
    23521 ctagcgttgg tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac
    23581 agcaatacgc ctgacaggaa agcaatgtca gtcccggaac gaataggggc atagtagtca
    23641 gccaccgccg ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga
    23701 attttggctt ccatcgccca gcggaacccg accgggtgag cttcagcggc attaccgccc
    23761 atcaccacga cgaggttggc attcttgatg tcgacccagt ggttggtcat cgcaccgcga
    23821 ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg
    23881 accgcgagca tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc
    23941 gacgcggaag cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg
    24001 ttttgcgcaa tgtagttcgc atcgcggtct tctttcatca gtttggcgat gcgatcaaac
    24061 gcctcttccc aactgatttg ttgccattta tcagaacctg gcgcacggta ttccggaaac
    24121 ttcaggcggc tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgct
    24181 ccgcggttga ccgggtgatc cgggtcacct tcgatatgga agatagatgc tttggcgttt
    24241 tttgctccgt caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag
    24301 gtattacggg tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagtgctacg
    24361 ctgggtgcaa aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta
    24421 aagaactgcc ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca
    24481 tttacattcg cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc
    24541 gcaaaggtcc agaattggct caatttccct ccatccagga agggtttgta acagaatacc
    24601 ataatgttgg tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa
    24661 gaaatcgaaa atgtgacaag tgtcacaggt gttcgtcaaa ttgagttatg gcggcgtgat
    24721 gatttacaac acccacggtt agatgaggtg gcggaagagg ttcccgttgc gctggtctac
    24781 aacggcattt cgcatgtagt gatgatggcg tcacccaaag accttgagta ctttgcgctc
    24841 ggtttttcgc tttccgaggg gattatcgaa agtccgcgcg atatcttcgg tatggatgtc
    24901 gttccttcct gtaatggtct ggaagtgcaa attgagcttt ccagccgccg ctttatgggg
    24961 ttgaaggagc gtcgccgggc actggcggga cgtacgggat gtggcgtgtg cggcgtggag
    25021 caacttaatg acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc
    25081 aacaaactgg atgatgcatt acgtcatctc aatgattttc agccagtggg gcaactgacc
    25141 ggttgtactc acgccgccgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa
    25201 gacgtgggtc gccatgtggc gctggataaa ctgttaggcc gccggtcaca agaaggggaa
    25261 agctggcagc aaggtgcggt gctggtttcc agccgtgcca gttatgaaat ggtgcaaaag
    25321 tcggcgatgt gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta
    25381 gaagtggctg agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg
    25441 gtttataccc atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta
    25501 aattacagga cttattcatt tcgtgaattt tgttattttt ttataaacaa aacgcgtgta
    25561 gtggcgttaa cgcctgcgct gcaacctatt gatggcgtgg cggtgagtta tattgatgct
    25621 gccgtagcgt tgggtaatac cattaacgaa atggataaat attacaccca ggaaaattat
    25681 aaagacgatg cgtttgagaa agggaaaaca ttccactact actctctggc agtcatgatt
    25741 tcagcgaaac aaattaataa cctgatatcg caagataagt ttgatgcaga agcggcaatg
    25801 aagaaagtgt ctgaactgga aacgctggtg gcgcaggcca aagaagcgga taaaagcggc
    25861 atgaatttct cgtttattaa ttcggcaggc cagtatcaac tcgaggctaa aaaatacgtt
    25921 cgccgcatca gagataaagt cccgtactct gactgggata aagagcaact tcaggacgca
    25981 aactcaagct ggatggccga agactcattt ccacgagcgt tatgcgatta caacgaaatg
    26041 gtcgatgaaa tatttcagtt aattgtaatc gcggggcggg tttgtgatga gcacggatat
    26101 gtcacgaagt cgtaacaata taaagcagag tgaaggataa attttgtgaa taaagtatca
    26161 ttatttaatg attttgtgaa tgttaaaatg tctcagataa tttgtttaag cgttatttat
    26221 atttgtggct tatgagtcag cgtgaatcat atctaatatt ttttacgata tttagttaaa
    26281 aacggcacta ttgttatatt cctcacgttc caggaaatca gcggatttac tcatattatg
    26341 cggcgggata ctatccatcg gcctgctgaa gttggattaa aaattcgaaa gtgcacacct
    26401 tatatttctc tatatacgta ttccttttgg aaagcgtagg gtctccttta tttcagggaa
    26461 cgtaaataat gaaaaaatcg acattatctt tagccatcgg tttattattg gcatgtagta
    26521 ccggtatggc aaaaacacag catttaacgc tggaacaacg catggcattg ctggaagaac
    26581 gcctggaagc ggcagaaatg cgggcagcaa aagcagaggg gcaggttaaa cagcttcaga
    26641 cacaacaagc cgccgagatc cgcgaaatta aaaccgcaca gggcaacacg ccggtaaacg
    26701 gtcaatcaac gacggagtca gagaagaaaa acgccacccc gcctaatctc ctgctttcag
    26761 ggtatggcga tttaaaaatc tacggtgacg tagaatttaa tatggatgcg gaaagtaatc
    26821 atggcctgct ggcaatgacc aacgctgatg tgaatagcga tcccactaat gaacagtgga
    26881 atctcaatgg tcgtattctg ttaggttttg atggtatgcg aaaactggat aatggctatt
    26941 tcgctgggtt ctccgcacaa ccgctggggg atatgcacgg ttcagtaaat atcgatgatg
    27001 cggttttctt ctttggcaaa gaaaacgact ggaaggtcaa agtaggccgt tttgaagcct
    27061 acgatatgtt cccgctgaat caggatacct ttgttgaaca ttccggtaat actgcgaacg
    27121 atctttatga cgatggcagc ggttatatct atatgatgaa agagggccgc ggacgttcta
    27181 acgctggcgg taatttcctc gtcagcaaac aactcgataa ctggtatttt gaattaaaca
    27241 cgttactgga agacggaaca tctttatata acgacggtaa ttatcatgga cgcgatatgg
    27301 aacagcagaa aaatgttgct tatctgcgtc cggtaattgc ctggtcgccg acggaagaat
    27361 tcaccgtttc cgcagcgatg gaagcgaatg tggtaaataa tgcttatggt tataccgata
    27421 gcaagggtaa ttttgtcgat cagtccgatc gtaccggtta tggcatgagt atgacctgga
    27481 atggcctgaa aaccgatccg gaaaatggca tcgtggttaa tcttaatacc gcctatttag
    27541 atgctaataa tgaaaaagat ttcacggcag ggattaacgc gctgtggaaa cgtttcgagc
    27601 tgggttatat ctatgcacat aataagattg atgaatttag tggcgtggtt tgtgataacg
    27661 attgctggat tgatgatgaa ggaacataca acattcacac cattcatgcg tcttatcagt
    27721 tcgctaatgt gatggatatg gagaacttta atatttacct cggcacgtat tactccattc
    27781 tggatagcga cggcgataag atacacggcg acgatagtga tgaccgttac ggcgcacgcg
    27841 ttcgctttaa atacttcttc tgatgaaagg gcggtgctgg atattttccg gcaccgcaac
    27901 tcattaaggc aggtaaaaca cttcttgcag ttcgctactg actgggctgt tatccgcatt
    27961 agcgggcata acgtcggtca tatatttcca ccaacgttgg caaacatctg tactggcaac
    28021 cgcattccac cgttcttcag attcaatctc taccgtggca aatagcagat tatgcgcttt
    28081 gtcgagatag atggcgtagt tatgcgcacc gtgagatttc agcactgctt ccagttccgg
    28141 ccagattgga ttatgccgac gctgatactc ttcgtgggcg tcggggttaa cctgcatgac
    28201 aaaggctttg cggatcatgt ttgctcctta cagcgccagc gcactggcga gtggtgtaac
    28261 gccgaaacgc tggccgagcg ctatcaactc ttcacggctg atggtctgtt tcatgccgcc
    28321 catcgaataa accttcacta atatttgtgc tgatttttct gcggtgtcga ttaaaccgaa
    28381 ggtttcatcc agtgtcggtc cggaaccgaa cacaccgtgg aagggccaca acaccagcga
    28441 atgtttttgc atctcttgtg cagttgcctg gccgatttcg tccgtgccgg gtaccatcca
    28501 cggcagaatg ccaacgccat ccgggaatac caccagacac tcggtgctgc cttcccacag
    28561 ttggcgagtg aagaccgcgg tgtcgttttc cagcacgtag gtgagagcga tcaggttggt
    28621 ggcgtggcag tgtatgatca cccgatcttt gccgttggtg gctttaatgc gctcgcagtg
    28681 ggagagaaag tgagccggaa gttcggaagt ggggacggct tcgttggtta acccccaaag
    28741 aatgtggtag cccgcgccgt cgctgtcgac ttttacgatg cctaagttag ccgcaggatc
    28801 aagctggacg ttacggaaga atttgccgga gccggtgaca ataaacggtg tatttgccag
    28861 taaaggcatg ggctggctga gcgggatata gcgcggttgt tggtgaaaat tgtcgtgata
    28921 tggtgcgata tcggcgtcat ccaggcgcag cgtcaggttg ccgccgttgc gctcgtccca
    28981 gcctttcagc caggcatcgg tggtggcttt gatcattccc tggacaaacc aggactgggt
    29041 tatgttttgc atgttctgtg ttcctgttaa ttggacgtcc gtgcctgttg ccggatgtac
    29101 gatctgtagg ccggataagg cacttgtgcc gcatccggca atcaatgcct gatgcgacgc
    29161 tgtcgcgtct tatcaggcct acaactgttg ctgacccgta ggccggataa ggcact
//
LOCUS       DAZFRE010000066        29102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFRE010000066 DAZFRE010000000
VERSION     DAZFRE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.066"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(118..1593)
                     /gene="ampG"
                     /locus_tag="ACHKUI_003191"
     CDS             complement(118..1593)
                     /gene="ampG"
                     /locus_tag="ACHKUI_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6599341.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1637..2215)
                     /gene="yajG"
                     /locus_tag="ACHKUI_003192"
     CDS             complement(1637..2215)
                     /gene="yajG"
                     /locus_tag="ACHKUI_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6599342.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2520..2837
                     /gene="bolA"
                     /locus_tag="ACHKUI_003193"
     CDS             2520..2837
                     /gene="bolA"
                     /locus_tag="ACHKUI_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6599343.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3181..4479
                     /gene="tig"
                     /locus_tag="ACHKUI_003194"
     CDS             3181..4479
                     /gene="tig"
                     /locus_tag="ACHKUI_003194"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6599344.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAVQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4725..5348
                     /gene="clpP"
                     /locus_tag="ACHKUI_003195"
     CDS             4725..5348
                     /gene="clpP"
                     /locus_tag="ACHKUI_003195"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6599345.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5474..6748
                     /gene="clpX"
                     /locus_tag="ACHKUI_003196"
     CDS             5474..6748
                     /gene="clpX"
                     /locus_tag="ACHKUI_003196"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6599346.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6936..9290
                     /gene="lon"
                     /locus_tag="ACHKUI_003197"
     CDS             6936..9290
                     /gene="lon"
                     /locus_tag="ACHKUI_003197"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6599347.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9481..9771
                     /gene="hupB"
                     /locus_tag="ACHKUI_003198"
     CDS             9481..9771
                     /gene="hupB"
                     /locus_tag="ACHKUI_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6599348.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9963..11834
                     /gene="ppiD"
                     /locus_tag="ACHKUI_003199"
     CDS             9963..11834
                     /gene="ppiD"
                     /locus_tag="ACHKUI_003199"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6599349.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            11985..12356
                     /gene="ybaV"
                     /locus_tag="ACHKUI_003200"
     CDS             11985..12356
                     /gene="ybaV"
                     /locus_tag="ACHKUI_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6599350.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12462..12860
                     /gene="fadM"
                     /locus_tag="ACHKUI_003201"
     CDS             12462..12860
                     /gene="fadM"
                     /locus_tag="ACHKUI_003201"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6599351.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLGPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12912..13607)
                     /gene="queC"
                     /locus_tag="ACHKUI_003202"
     CDS             complement(12912..13607)
                     /gene="queC"
                     /locus_tag="ACHKUI_003202"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6599352.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13672..15372)
                     /gene="ybaE"
                     /locus_tag="ACHKUI_003203"
     CDS             complement(13672..15372)
                     /gene="ybaE"
                     /locus_tag="ACHKUI_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6599353.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPDWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLEATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15472..16290
                     /gene="cof"
                     /locus_tag="ACHKUI_003204"
     CDS             15472..16290
                     /gene="cof"
                     /locus_tag="ACHKUI_003204"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6599354.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLEAYLITGNGTRVHSLEGELLHRDDLPADVSELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16443..16901
                     /gene="decR"
                     /locus_tag="ACHKUI_003205"
     CDS             16443..16901
                     /gene="decR"
                     /locus_tag="ACHKUI_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6599355.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16931..18703
                     /gene="mdlA"
                     /locus_tag="ACHKUI_003206"
     CDS             16931..18703
                     /gene="mdlA"
                     /locus_tag="ACHKUI_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6599356.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18696..20477
                     /gene="mdlB"
                     /locus_tag="ACHKUI_003207"
     CDS             18696..20477
                     /gene="mdlB"
                     /locus_tag="ACHKUI_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6599357.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAVVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20658..20996
                     /gene="glnK"
                     /locus_tag="ACHKUI_003208"
     CDS             20658..20996
                     /gene="glnK"
                     /locus_tag="ACHKUI_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6599358.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21026..22312
                     /gene="amtB"
                     /locus_tag="ACHKUI_003209"
     CDS             21026..22312
                     /gene="amtB"
                     /locus_tag="ACHKUI_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6599359.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22361..23221)
                     /gene="tesB"
                     /locus_tag="ACHKUI_003210"
     CDS             complement(22361..23221)
                     /gene="tesB"
                     /locus_tag="ACHKUI_003210"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6599360.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23439..24011
                     /gene="ybaY"
                     /locus_tag="ACHKUI_003211"
     CDS             23439..24011
                     /gene="ybaY"
                     /locus_tag="ACHKUI_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6599361.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24042..24353)
                     /locus_tag="ACHKUI_003212"
     CDS             complement(24042..24353)
                     /locus_tag="ACHKUI_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6599362.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24516..24612
                     /gene="ffs"
                     /locus_tag="ACHKUI_003213"
     ncRNA           24516..24612
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKUI_003213"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24732..25085
                     /gene="ybaA"
                     /locus_tag="ACHKUI_003214"
     CDS             24732..25085
                     /gene="ybaA"
                     /locus_tag="ACHKUI_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO6599363.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25127..26677)
                     /gene="pdeB"
                     /locus_tag="ACHKUI_003215"
     CDS             complement(25127..26677)
                     /gene="pdeB"
                     /locus_tag="ACHKUI_003215"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6599364.1"
                     /translation="MRTRHLVSLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHSDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDIQP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26841..27311)
                     /gene="ylaC"
                     /locus_tag="ACHKUI_003216"
     CDS             complement(26841..27311)
                     /gene="ylaC"
                     /locus_tag="ACHKUI_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6599365.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27427..27978)
                     /gene="maa"
                     /locus_tag="ACHKUI_003217"
     CDS             complement(27427..27978)
                     /gene="maa"
                     /locus_tag="ACHKUI_003217"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6599366.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28151..28369)
                     /gene="hha"
                     /locus_tag="ACHKUI_003218"
     CDS             complement(28151..28369)
                     /gene="hha"
                     /locus_tag="ACHKUI_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO6599367.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28395..28769)
                     /gene="tomB"
                     /locus_tag="ACHKUI_003219"
     CDS             complement(28395..28769)
                     /gene="tomB"
                     /locus_tag="ACHKUI_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6599368.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7339 a         6972 c         7838 g         6953 t
ORIGIN      
        1 caataatttc caaacgctaa gccgcacaaa agaacaaata ttaataagcg atgctgatgt
       61 ttatttagcc gtaataatta cggcgagtga ttactacagc taaataatat ttacagatta
      121 cgtcagatgt gtttttcgta gcgccagata atccagcaaa ccaccaagca caacgccaga
      181 aagggcgact aaaaccccga cttccagcag cgcaggcagg aaagagaagt gcgtcaaatc
      241 cagcgcgtcc attgtcaaca gcagtaacca cacggccaac aggctgatac ccgccgccag
      301 tgtccacatg gcaaaggcat aacctgcagg atattcggta cgggagataa agttgccatt
      361 tactcgtgta tattcaagcg tctggcggca aaccagcagc aaaataagcc ctggtacggc
      421 agcggcgacg gagaatagat agaatgtcga ccagccgtgt gcttcaacaa accaacccgc
      481 cacggggccg acataaactc gccctacagc agaaagcgct gagagcaggg caaattgagt
      541 agcggaaaat gacttattac acagcgtcat taacagcgcg acaaaggctg atgtacccat
      601 cccgccacag aggttttcga aaaagacggc tgcacccatg ctgtagagat gcttatcagt
      661 aatcgacagc agccagtaac cggcgttaga tgcaccttgt aaaatgccga aaatcagcag
      721 tgcacggaac agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc caacaatggt
      781 cgccagtaag ccaagcgttt tgttaaccac gcctacttca cccgcatcaa acccgacgcc
      841 gcgaatcaaa aacgtggttg tcaggctcat agcgaatgcg tcgcccagct tatacagcac
      901 gataagaagc aaaataagcc aggcattatt gcgaccaaag aaatctcgca gaggtgcaac
      961 aaccgcttgt tccagcgttt tgggcacagg aatggtgtcg gtgggttctg gtgcaagcaa
     1021 cgtcgcgata atacagggga tcaacagcgc cgccatcaac cagtacatgc cctgccagcc
     1081 cagccattta tctgccagcc acagggctaa gccgccggaa accagcatcc ctaaacggta
     1141 acccagcacg ctgattgccg cgcccgcacc acgttcttct gccgggagca catcggtttt
     1201 ccacgcatcg aagacaatat cctgggaggc agagcaaaaa gcgatcacca ccgccagtgc
     1261 cgccatccag cggagctggg tgcctggttc gagaaaaccc atcgccgcaa tggcgactaa
     1321 taacagaatt tgcgtggcga gcagccaacc gcgccgccgc ccgaaaaatg ggggcgtgta
     1381 gcggtccatc agtggtgacc agaggaattt aaaaacgtaa gcctggccta ccagagagaa
     1441 gaaaccaatg gttttgagat cgatattctc gaccgtcatc caggcctgta aggtgccgga
     1501 cgtcagggcg agcggtagcc cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg
     1561 ctgttgaaaa atacgtaaat attgactgga catgggcgtc tacaggccca gcttgcaccg
     1621 ggccagaggg cagaaattaa cgcgcgttct gcttgatgaa ttcgtggatg ctggtgtcct
     1681 gagacatatc agcgatggta tcggtcagca cactgttaac cgcatcggcg atatttttgt
     1741 tggaggcctg gaatgcacct tcaacgttgt agctggcacg atagtttttg gtcattttgt
     1801 tgccattctg cgcggtagcg atgatggcga tatccgcttt ggtcgcgatg ttgtagcgca
     1861 cgttgccctg ggacacgtca gcatacagtt ggctaacgat gatttgcaga ttaaccgggc
     1921 cattcggacc aaccatgtat ccacgggcgg tcatctgttt ttccagcact tcttgcagca
     1981 ggaaacgcag atcgcgggag gcggtcaggg taacgatttg attatcgcgg gtgacttttg
     2041 ccagcgcctg atcggtacgc tgatcggcac cattaatgct tacggtgacg cccatcaggc
     2101 ttggatcctg ctgtggcagt gtaatcgtcg gggaaacttc gatagttgtt ggcggttttg
     2161 cgcatcctgc aagcataaac agagcaacta acgggaagag gatttttttg aacatgttcg
     2221 ggctctcaga gactcttaag cgtgtttggt aaaaattccc gccatcataa cattgccaac
     2281 ggcgagggga agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa tcgcccctgg
     2341 taaaagaaac gctgatgcga ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc
     2401 ttgacggaaa aaccaggacg aaacctaaat atttgttgtt aagctgcaat ggaaacggta
     2461 aaagcggcta gtatttaaag ggatggatga catctcagcg ttgtcggagg agatatttca
     2521 tgatgatacg tgagcggata gaagaaaaat taagggcggc gttccaaccc gtattcctcg
     2581 aagtagtgga tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc cattttaaag
     2641 ttgtgctggt cagcgatcgt tttacgggtg aacgttttct gaatcgtcat cgaatgattt
     2701 acagtacttt agcggaggaa ctctctacta ccgttcatgc gctggctctg catacttaca
     2761 ctattaagga gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag
     2821 caggaagcat cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt atgttgctaa
     2881 agattttatg aaaaacggcc tgcgggccgt tttgttttgt ctggattttg cgctttttgc
     2941 ccagcattca gacgaaaatc gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa
     3001 ccgttctcga ctcataaaag tgatgccgct ataatgccgc gtcctatttg aatgctttcg
     3061 ggatgattct ggtaacaggg aatgtgattg attataagaa catcccggtt ccgcgaagcc
     3121 aacaacctgt gcttgcgggg taagagttga ccgagcactg tgattttttg aggtaacaag
     3181 atgcaagttt cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct
     3241 gctgacagca tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt
     3301 attgacggct tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg
     3361 tctgtacgcc aggacgtcct gggcgacctg atgagccgta acttcattga cgccatcatt
     3421 aaagaaaaaa tcaatccggc tggcgcaccg acctatgttc cgggcgaata caagctgggt
     3481 gaagacttca cctactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg
     3541 gaagcgatcg aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt tgacggcatg
     3601 ctggatactc tgcgtaaaca gcaggcaacc tggaaagaaa aagacggcgc tgttgaagca
     3661 gaagaccgtg tgaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt
     3721 aaagcgtctg atttcgtact ggcgatgggt caggggcgca tgatcccggg ctttgaagac
     3781 ggtatcaaag gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagag
     3841 taccacgcag aaaacctgaa gggtaaagca gcgaaattcg ctatcaacct gaagaaagtt
     3901 gaagagcgtg aactgccgga actgactgca gaattcatca aacgtttcgg cgttgaagat
     3961 ggttccgtag aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc
     4021 gccatccgta accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc
     4081 gacgtaccgg ctgcgctgat cgacagcgaa atcgacgttc tgcgtcgcca ggctgtacag
     4141 cgtttcggtg gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt cgaagaacag
     4201 gctaaacgcc gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac caacgagctg
     4261 aaagctgacg aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc gtacgaagat
     4321 ccgaaagaag ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa catgcgcaat
     4381 gttgctctgg aagaacaggc tgttgaagct gtactggcga aagcgaaagt gactgaaaaa
     4441 gaaaccactt tcaacgagct gatgaaccag caggcgtaat ttacgtagca taacgcgcta
     4501 aattcgcaca aaggcccgtc accgccaggt ggtgggcttt tttttgtcat gaattttgca
     4561 tggaaccgtg cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg
     4621 aaatatggtg atgccgtacc cataacacag ggactagctg ataatccgtc cataaggtta
     4681 caatcggtac agcaggtttt ttcaatttta tccaggagac ggaaatgtca tacagcggcg
     4741 aacgagataa ctttgcaccc catatggcgc tggtgccgat ggtcattgaa cagacctcac
     4801 gaggtgagcg ctcttttgat atctattctc gtctacttaa ggaacgcgtc atttttctga
     4861 ctggccaggt tgaagatcac atggctaacc tgattgtggc gcagatgctg ttcctggaag
     4921 cagaaaaccc agaaaaagat atctatctgt acattaactc tccaggcggg gtgattactg
     4981 ccgggatgtc tatctatgac accatgcagt ttatcaagcc tgatgtcagc accatctgta
     5041 tgggccaggc ggcctcgatg ggtgctttct tgctgactgc aggggcaaaa ggtaaacgtt
     5101 tctgcctgcc gaattcgcgc gtgatgattc accagccgtt gggcggctac cagggccagg
     5161 cgaccgatat cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc atgaatgaac
     5221 ttatggcgct tcatacgggt caatcattag aacagattga acgtgatacc gagcgcgatc
     5281 gcttcctttc tgcccctgaa gcggtggaat acggtctggt cgattcgatt ctgacccatc
     5341 gtaattgatg ccagaggcgc aactgtgccg ctatacttat ccagggcggc acaacgctgt
     5401 aagcggcttg cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa
     5461 gaggttttga cccatgacag ataaacgcaa agatggctca ggcaaattgc tgtattgctc
     5521 tttttgcggc aaaagccagc atgaagtgcg caagctgatt gccggtccat ccgtgtatat
     5581 ctgcgacgaa tgtgttgatt tatgtaacga catcattcgc gaagagatta aagaagttgc
     5641 accgcatcgt gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc acctggacga
     5701 ttacgttatc ggtcaggaac aggcgaaaaa agtgctggcg gtcgcggtat acaaccacta
     5761 caaacgtctg cgcaacggag ataccagcaa tggcgtcgag ttgggcaaaa gtaacattct
     5821 gctgatcggt ccgaccggtt ccggtaaaac gctgctggcc gaaacgctgg cgcgcctgct
     5881 ggacgttccg ttcaccatgg ctgacgcaac cacgctgacc gaagccggtt atgtgggcga
     5941 agacgttgaa aacatcattc agaagctgtt gcagaagtgc gattacgacg tacagaaagc
     6001 gcagcgcggg attgtctaca tcgatgaaat tgacaagatt tctcgtaagt cagacaaccc
     6061 gtccattacc cgagacgttt ccggtgaagg cgtacagcag gcactgttga aactgatcga
     6121 aggtacggta gctgctgttc cgccgcaagg tgggcgtaaa catccgcaac aggaattctt
     6181 gcaggttgat acctctaaga tcctgtttat ctgtggcggt gcgtttgccg gtctggataa
     6241 agtgatttcc catcgtgtag aaaccggttc cggcattggt tttggcgcga cggtaaaagc
     6301 gaagtccgac aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg aagatctgat
     6361 caagtttggt ctgatccctg agttcattgg tcgtctgccg gttgtcgcaa cgttgaatga
     6421 actgagcgaa gaagctctga ttcagatcct caaagagccg aaaaacgccc tgaccaagca
     6481 gtatcaggcg ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga
     6541 tgctatcgct aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc gttctatcgt
     6601 agaagccgca ctgctcgata ccatgtacga tctgccgtcc atggaagatg tcgaaaaagt
     6661 ggttatcgac gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt atggtaagcc
     6721 ggaagcgcaa caggcatctg gtgaataatt aaccattccc atacaattag ttaaccaaaa
     6781 aggggggatt ttatctcccc tttaattttt cctctattct cggcgttgaa tgtgggggaa
     6841 acatccccat atactgacgt acatgttaat agatggcgtg aagcacagtc gtgtcatctg
     6901 attacctggc ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt tctgaacgca
     6961 ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct
     7021 tatttgtcgg gcgggaaaaa tccatccgtt gtctggaagc ggcgatggac catgataaaa
     7081 aaattatgct ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt
     7141 tcactgtcgg gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca
     7201 aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac
     7261 acttttctgc gaaggcggag tatctggagt cgccgaccat tgatgagcgg gaacaggaag
     7321 tgctggtgcg tactgcaatc agccagttcg aaggctacat caagctaaac aaaaaaatcc
     7381 caccagaagt gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca
     7441 ttgctgcaca tatgccgctg aaactggctg acaaacagtc cgttctggag atgtccgacg
     7501 ttaacgaacg tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg
     7561 ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact
     7621 atctgaacga gcaaatgaaa gctattcaga aagaactcgg tgaaatggac gacgcgccgg
     7681 acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag
     7741 agaaagcgga agcagagctg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga
     7801 ccgtagtgcg tggatatatc gactggatgg tacaggtgcc gtggaatgcg cgtagcaagg
     7861 tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac cgaccattat ggtctggagc
     7921 gcgtgaaaga tcgtatcctt gagtatcttg cggttcaaag ccgtgtcaac aaaatcaagg
     7981 gaccgatcct ctgcctggta gggccgccgg gggtaggtaa aacctccctg ggtcagtcca
     8041 tcgccaaagc caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag
     8101 cggaaatccg tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ctgatccaga
     8161 aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt
     8221 cttctgacat gcgtggcgat ccggcctccg cactgcttga agtgctggat ccagagcaga
     8281 acgtagcgtt cagcgaccac tacctggaag tggattacga tctcagcgac gtaatgtttg
     8341 tcgctacgtc gaactccatg aacattccgg caccgctgct ggatcgtatg gaagtgattc
     8401 gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga
     8461 agcagattga acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta
     8521 tcggcattat tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct
     8581 ccaaactgtg tcgcaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg
     8641 aaattaacgg tgataacctg catgactacc tcggtgttca gcgtttcgac tatgggcgcg
     8701 cggataacga aaaccgtgtc ggccaggtaa ccggtctggc gtggacggaa gtgggcggtg
     8761 acttgctgac cattgaaacc gcgtgcgttc cggggaaagg taaactgacc tataccggtt
     8821 cgctcggcga agtgatgcag gagtctattc aggcggcgtt aacagtggtt cgtgcgcgtg
     8881 cggaaaaact ggggatcaac cctgattttt acgaaaaacg tgatatccac gtccacgtac
     8941 cggaaggtgc gacgccgaaa gatggtccga gtgccggtat tgccatgtgc accgcgctgg
     9001 tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc
     9061 tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa actactggca gcgcatcgcg
     9121 gcgggattaa aacagtgtta attccgttcg aaaataaacg cgatctggaa gagattcctg
     9181 acaacgtaat tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc
     9241 tggcgctaca aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag tgacctcgcg
     9301 caaaatgcac taataaaaac agggctggca ggctaattcg ggcttgccag cctttttttg
     9361 tctcgctaag ttagatggcg gatcaggctt gcccttatta aggggtgttg taaggggatg
     9421 gctggcctga tataactgct gcgcgttcgt accttgaagg attcaagtgc gatataaatt
     9481 ataaagagga agagaagagt gaataaatct caattgatcg acaagattgc tgcaggggct
     9541 gatatctcta aagctgcggc tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa
     9601 tctctgaaag aaggggatga tgtagcactg gtaggttttg gtacttttgc cgttaaagag
     9661 cgtgctgccc gtactggccg caacccgcag accggtaaag agatcaccat cgctgctgct
     9721 aaagtaccga gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc
     9781 ccagtgggga tgtgacgaag ttcaagggcg catctactga tgtgcctttt ttatttgtat
     9841 tcggtgactt tctgcgtctt gtgggctgac aattgccccc gtttcttgtc acaataggcc
     9901 tttgcgcgca tcgatacgtt gcgtgaggta cacagtcatc tacagcggag tgttgttaca
     9961 ccatgatgga cagcttacgc acggctgcaa acagtctcgt gctcaagatt attttcggta
    10021 tcattatcgt gtcgttcata ttgaccggcg tgagtggtta cctgattggc ggaggcaata
    10081 actacgccgc aaaagtgaat gaccaggaaa tcagccgtgg gcaattcgag aacgccttca
    10141 acagcgagcg taatcgcatg cagcaacagc tgggcgatca atactccgag ctggcagcga
    10201 acgaaggcta tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc
    10261 ttctggatca gtacgctcgt gagctgaaac tgggtatcag cgatgagcag gttaaacagg
    10321 cgattttcgc gaccccagcc ttccaggttg acggcaaatt tgataacagc cgctataacg
    10381 gtatcctcaa ccagatgggg atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc
    10441 tcactaccca acagctgatt aacggcgttg ccggtaccga ttttatgctg aaaggtgaaa
    10501 ccgacgagct ggcggcactg gtagctcaac aacgcgtggt gcgtgaagcg actatcgatg
    10561 ttaacgcgct ggcggcgaag cagcctgtga ccgaacagga aattgccagc tactacgaac
    10621 aaaacaaaaa caatttcatg acgccggaac aattccgcgt gagttacatc aagctggatg
    10681 ccgcgacgat gcagcaaccg gttagcgatg cggatatcca gagctactac gatcagcatc
    10741 aggatcaatt cacccagccg cagcgtaccc gttacagcat catccagacc aaaactgaag
    10801 atgaagcgaa agcggtactt gatgagctga ataagggcgg tgattttgct gcattagcca
    10861 aagaaaaatc tgccgatatt atctctgccc gcaacggcgg tgatatgggt tggttagaag
    10921 atgccactat cccggacgaa ctgaaaaatg cgggtctgaa agaaaaaggc caactgtctg
    10981 gtgttatcaa atcttcggtc ggtttcctga ttgtgcgtct ggacgacatt cagccagcga
    11041 aagtgaaatc gttagacgaa gtacgtgacg acattgcggc gaaagtgaaa cacgaaaaag
    11101 ccctcgatgc gtactacgcg ctgcagcaga aagtgagcga tgcggcaagc aacgacaccg
    11161 agtctctggc cggtgcagag caagctgccg gcgttaaagc cactcagacg ggttggttca
    11221 gcaaagataa cctgccggaa gagttgaact tcaagccagt tgccgacgct atcttcaacg
    11281 gcggtctggt aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc accgtagacg
    11341 gcgaccgcgc attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg aaaccgttgg
    11401 cagatgttca ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa caacaggcga
    11461 aagtggatgc tgagaaactg ctggttgatt tgaaagcagg caaaggtgcg gaagctatgc
    11521 aggctgccgg tctgaaattt ggcgagccga aaaccttaag tcgttccggt cgtgacccga
    11581 ttagccaggc tgcgtttgca ctgccgctgc cagcgaaaga caaaccgagc tacggtatgg
    11641 cgaccgatat gcaaggtaat gtagttctgc tggcgctgga tgaagtgaag caaggttcaa
    11701 tgccggaaga tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac gcacaaatcg
    11761 tctttgaagc tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgc
    11821 tggaacagca ataatcctga agccgcctcg caaaaaaatg ctttgcagct gtaacaagaa
    11881 aaggtcgctt tcgcggcctt ttccatttcc gaacattgcc atttgtttac tgttttcact
    11941 gccgttaagg tgattccact gttaacaaac aaggagaaaa cagtatgaaa cacggaatta
    12001 aagcactgct cattaccctg tccctggcct gtgccggaat gtctcatagt gctctggcgg
    12061 cagctcctgc ggcaaaaccg acaacggtag aaaccaaagc ggaagctcct gcagcacaaa
    12121 gtaaagcagc agttccggcg aaagccagtg acgaagaagg cactcgggtc agcattaata
    12181 atgccagcgc ggaagagtta gcccgcgcga tgaatggcgt tggcctgaag aaagcgcagg
    12241 cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat ctaaagcagg
    12301 tgccggggat gggcaattcg ctggtggaac gtaatctggc ggtattaacc ctgtaattaa
    12361 tttgcatagt ggcaattttt tgccagactg aagaggtcat accagttatg acctctgtac
    12421 ttataacagt tcttataaca acaacgtaag gttattgcgc tatgcaaaca caaatcaaag
    12481 ttcgtggata tcatctcgac gtttaccagc acgtaaacaa cgcccgctac cttgaatttc
    12541 tcgaagaagc ccgctggcat gggttggaaa atagcgacag ttttcactgg atgacggccc
    12601 ataacatcgc tttcgtcgtg gtcaatatca atattaacta tcgccgcccg gcggtattaa
    12661 gcgacctgtt aaccattacc agtcagttgc agcaattaaa cggtaaaagt ggcatcttaa
    12721 gccaggtcat tacactgggg ccggaaggac aggtggtagc ggatgcgctt attacgtttg
    12781 tttgtattga tcttaaaacg cagaaagcat tagctctgga aggggaattg cgcgaaaagc
    12841 tggagcagat ggttaagtaa acgttttgtg gtgccggatg ctcaagccgc atccggcgac
    12901 acccggaata attacttcaa cccggttttc tgcttcatcg ctgccatcac cgtcggttta
    12961 tcggccagat aatgattcaa accgttggca cgtaaattac acgccgcaca atgaccgcaa
    13021 ccgtcgcctt taatgccgtt ataacaggtc agcgtttcgt tacggactaa atccagcttg
    13081 ccgtaataat ctgccagcgc ccaggtttcc gctttatcaa tccacatcag cggcgtttca
    13141 aaacgaatat ctttcgccat gcccaaactg acggcatggt ttagtgcttt cacaaactca
    13201 tcgcggcaat ccgggtagcc ggagaaatcc gtttcgcaga cgccggtaat tacggcttct
    13261 gcttttacct gatacgcata tattgccgcc agtgtcagga acaaaatatt acgccctggg
    13321 acaaacgtat tcgggatacc atcggcttca ggttcataat caggcaccgg aatgctgtca
    13381 cgcgtcaggc tgctgaccgc cagctcgttg agcagcgtta catcaagcac cttatgcgcg
    13441 cgtgccccca gtttcagcgc cagttcgcgt gccacgtcga tttctgcgcg atggcgttga
    13501 ccgtaatcga acgtcacgca atggacttca tcatattgtt gtaatgcctg caccagacag
    13561 gtggtggaat cctggcctcc actgaacaca acgacagcac gtttcataga taatcctgct
    13621 tgaacaataa aaacgttatg gtaacgcctg cgattaaccc ggaccagcta ttcattgcga
    13681 cggcgcggga agccaggctt cggtaaattc aaaccagccg cgcggtgtca gtcgcacacc
    13741 gttcacgccg ggaggggcgc taatgcgata gtgatagttg aacagcggcg tcagcgtcgc
    13801 atctgccatt aactggctaa aaaccgcttt cagggcatta aatcggtttt cctcatcagg
    13861 cattacttgc accgcgtcca gtgtcgattg caaatgtgca tatgctggag cgtcgaaaac
    13921 atgtggccac agtggatcgc aacgcagcca ttgctccagg gtatattccg gtgcttcgcc
    13981 aattaatctg tcgcccatca tgaggtctgc gtgtgccagt agggtcgtgt cgtcccagtt
    14041 ttttgcgtta tgaaaaataa ttgtgagttc acagccttcc gctgccagtg tcgcctctag
    14101 gcgttctgcc atggtatgaa gttctatcgg taggtgataa accagcgtca aggttttcgg
    14161 tagtttaact tcatccggta cttgccattg tggaatagtc cagtctggca gtaatgcatg
    14221 actggcggtg atcaggtttt ctccgacttc taacgtttgt aataaaccgg attgatgaat
    14281 aatggagatc acttttcgcg cctgccagag ggacagtcgg ggacttttgc gcaacgttaa
    14341 atagcaaaaa cctaaactga tgccactact gacctggctg acccgttgca gctcctcagg
    14401 tttgccgata gtgatttgca cgggatgccg acaactggtt cccaaatctt tttcgaaaag
    14461 cggaggagtt atccagtact caaccgcttt aagcagcgga tgacgtaaat ggtaataatc
    14521 atggctttcc aggcgcacca gttccgctgt gaattgtgtt aagcgaaaag gaccagtgcc
    14581 gatcaggggg aattgcggat gcgccagatg gctgcaatag ctcgccagcc ggtgcgcaag
    14641 ccagtaatcg gggcgatgta aaaagaaggt cagacactgc ggatgggtga cttcaatacg
    14701 cttcacgcta ataaataatt gatccagtgc tggcagttgt aacagcatca ataaccgctg
    14761 gtgtaagtgg gaggctttta ctgcatcgcc gttatgccag tgtaaggttg agcgaagata
    14821 aaaatcccag cgtaacccgt cagtagaggt ttcccagtga tgcgctaaat cgccaatcgg
    14881 gcgctgggta ttattatcga agcgggtcag gccggaaaat atctgtccgg cgagatgctg
    14941 ctcggcacga ccgggcaaaa agcccggttg tagcggttcg agcgggcgat agtagggaat
    15001 acgcaacgtg ggcgtatcgt tttgccattg tccgcccata aacggctgta acagagtgcg
    15061 cagctcacct ggggccagtt gcgccagctc cagcacatct tgctgctttc cggtttccag
    15121 cgcctgttcc atcatcgcat tgcgtagcga ttctggcgtg accagaaagc gtaattgtcc
    15181 gcgctttccg cgtcctgact gcgcctgcca ctccagccat cccgcctcct gtgcctgacg
    15241 caacagcgta cgaacatggc gttcgctgca aaaacagcgt tcggccagtt cgctgacggt
    15301 gacggtttgc ggctttccgg cggaaggttg ccacagacgt tgatactggt taagacggtt
    15361 gagcagtcgc atataaaccc ggaacaatat tatttaacta ttcactatta cttccgtata
    15421 tatcaggtga tactcaatca ccattaaccg tgtcacagag tggagaaaga aatggctcgt
    15481 ctggcagcat ttgatatgga tggcacttta ttgatgcctg accatcattt aggtgagaaa
    15541 accctctcta ctttggcgcg actgcgtgaa cgcgacatta ccctcacttt tgccacgggg
    15601 cgtcatgcgc tggagatgca gcatattctc ggggcgctat cgctggaggc atatttgatt
    15661 actggcaacg gaacgcgcgt gcattctctg gaaggtgaac ttttacatcg tgatgattta
    15721 cctgcggatg tctcggagct ggtgctgtat cagcaatggg atacccgagc aagcatgcat
    15781 atcttcaatg acgatggttg gtttaccggg aaagagatcc ctgcgttgtt gcaggcattt
    15841 gtctatagcg gttttcgtta tcagataatc gatgtcaaaa aaatgccact cggcagcgtc
    15901 accaagatct gcttctgtgg cgatcacgac gatcttacac gcttgcagat ccagctatac
    15961 gaagcattag gcgagcgtgc acatttatgt ttttccgcca cggattgcct cgaagtgctg
    16021 ccggtgggct gcaataaagg cgctgcattg acggtgctga cccaacattt aggtttatcg
    16081 ttacgcgatt gcatggcctt tggtgatgcg atgaacgatc gcgaaatgtt aggcagcgtc
    16141 ggtagcggat ttattatggg caatgcgatg ccgcaactgc gcgcggagct cccgcattta
    16201 ccggtgattg gacattgccg aaatcaggct gtctctcact atttgacgca ctggctggac
    16261 tatccacatc taccttattc ccccgaataa cgagatccct tccagcaccg ggcaattgcc
    16321 cggttttttt tgcgttgaat ttgtcatttt gtgccgtggt gcttaaaccg cacagaataa
    16381 attgtcgcga tttcaccttt aaaatagaat taaaagagaa aaaaattctc tgtggaaggg
    16441 ctatgttaga taaaattgac cgtaagctgc tggccttgct acagcaggat tgcaccctct
    16501 ctttgcaggc actggctgaa gccgttaatc tgacaaccac cccctgctgg aagcgtctga
    16561 aacggctgga ggacgacggt atccttatcg gcaaagtcgc cctgctggat ccggaaaaaa
    16621 taggcctcgg cctgaccgcc tttgtgctga taaaaacgca acatcacagc agcgaatggt
    16681 attgccgctt tgtcacggtg gttaccgaaa tgccagaagt gctggggttc tggcgcatgg
    16741 ctggtgaata cgattatctg atgcgcgtcc aggttgccga catgaaacgc tacgacgagt
    16801 tttataagcg tctggtaaac agtgtaccgg ggctgtcgga cgtcacttcc agcttcgcga
    16861 tggaacagat taaatacacc acttctttac ccatcgaata aatatccaga atcaagtcag
    16921 gacacaacgc gtgcgattat ttgctcaatt aagctggtat ttccgtcggg aatggcgtcg
    16981 ctatctcggg gctgtcgcct tgcttgtcat tatcgcgatg ctgcaactgg ttccgccaaa
    17041 agtggttggt attgttgttg atggcgtgac agaacaacac tttactaccg ggcagatcct
    17101 gatgtggatc gccaccatgg tgctgattgc cgttgtggtt tatctcctgc gttacgtctg
    17161 gcgcgtattg ctgtttggtg cgtcttatca actggctgtt gaactgcgtg aagattatta
    17221 ccgccagcta agccggcagc atcctgagtt ttacctgcgt catcgcacgg gtgacctcat
    17281 ggctcgtgcg accaatgacg tcgatcgcgt cgtgtttgcc gccggagaag gggtgctgac
    17341 gctggtggat tcactggtaa tgggctgcgc tgtgttgatt atgatgtcta cgcaaattag
    17401 ctggcagttg accttatttg ccctgttgcc gatgccagtg atggcgatca tgatcaagcg
    17461 caacggcgat gccttgcatg aacgctttaa gctggcacag gcggcgtttt ccagtcttaa
    17521 tgaccgcacc caggaaagcc tcaccagtat ccgcatgatc aaagcctttg gtctggaaga
    17581 tcgtcagtcg gcgttatttg ccgcggatgc cgaagatacc ggcaaaaaaa acatgcgggt
    17641 ggcgcgtatt gatgctcgtt tcgacccgac catctatatc gcgattggta tggcgaactt
    17701 gctggcgatt ggcggcggta gttggatggt ggtacagggc agtttaacgc tgggccagct
    17761 caccagtttt atgatgtatt taggtctgat gatttggcca atgctggcgc tggcatggat
    17821 gtttaacatt gtggaacgtg gcagcgcggc gtatagccga attcgtacca tgctggccga
    17881 agcgccagtc gttattgatg gtagtgataa ggtgccagaa ggtcgtggtg aactggatgt
    17941 gaatattcgc cagtttacgt atccgcagac tgaccatcct gcgctggaaa acgtcaattt
    18001 cgccctgaaa cccggtcaga tgctgggtat ctgcgggccg actggttccg gcaaaagtac
    18061 cctgttgtcg ctcattcagc gtcatttcga cgtcagcgag ggtgatatcc gttttcatga
    18121 tattcctctg acgaagttac aactcgatag ctggcgtagc cgtctggcgg tggtcagcca
    18181 gacaccattc cttttttccg acaccgtggc gaacaacatc gcgttgggtt gcccgaatgc
    18241 cacccagcaa gagattgaac acgtagcgcg gttagccagc gtacatgacg atattttgcg
    18301 tctgccgcaa ggttacgaca cagaagtggg cgagcgcggc gtgatgcttt ctggcggaca
    18361 aaaacagcgt atctccattg ctcgagcgtt attagtcaac gcggaaatcc tcatccttga
    18421 cgatgcgctt tcagcggtgg acggacgcac agagcaccag atcctgcata acctgcgtca
    18481 gtgggggcag ggaagaacgg taatcatcag tgcccatcgc ctttctgcac tgacggaagc
    18541 cagtgaaatt attgtgatgc agcacgggca tatcgcccag cgtggcaatc atgatgtgct
    18601 ggtacaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg aggcagcgct
    18661 cgacgacgcc ccggaaattc gcgaggaggc tgtcgatgcg tagttttagc caactgtggc
    18721 cgactctcaa gcgtctgtta gcgtacggtt caccgtggcg taaaccgttg gggattgcgg
    18781 tcctgatgat gtgggttgcg gcggcggcag aagtcagtgg gccgctgctt atcagctatt
    18841 ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa agtggttgca gggctggcgg
    18901 cggcgtatgt tgggctgcaa ctgtttgcag ccgggctaca ttacgcgcag tcgctgctgt
    18961 ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg gatgctgcgt
    19021 tacgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt tcccgcgtca
    19081 ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact gtcctgcgta
    19141 gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg cgaatggcgc
    19201 tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatat cagcgttaca
    19261 gcacgccgat tgtccgtcgt gtgcgcgctt atctggcgga tatcaacgac ggctttaacg
    19321 aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga tttggcgaac
    19381 gtatgggtga ggccagtcgt tcacactata tggcgaggat gcaaaccctg cgcctcgacg
    19441 gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt ggcttgttga
    19501 tgctgtttgg cttctccgcc agcggcacca ttgaagtggg cgtgttgtat gcgttcatca
    19561 gttatttggg gcgacttaac gaacccttaa tcgaactgac cacgcagcag gcgatgctgc
    19621 aacaggctgt tgttgctggt gagcgcgttt ttgagctgat ggacggaccg cgccagcaat
    19681 atggcaatga tgatcgcccg ttgcagagcg gcaccatcga agtcgataac gtgtcatttg
    19741 cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct tcgcgcaact
    19801 ttgtggcgct ggtcgggcat accggcagtg gcaaaagcac cctcgccagt ttattgatgg
    19861 gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta agtacgctaa
    19921 gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg gtgctggcgg
    19981 ataccttcct cgccaacgtg acgctgggac gtgatatctc tgaagaacgc gtctggcagg
    20041 cgctggaaac cgtgcagctg gcggagctgg cgcgtagcat gagtgatggt atttacacgc
    20101 cgcttggcga gcaggggaat aatctctcag tcgggcaaaa gcaactgctg gcactggcgc
    20161 gcgtgctggt ggagacgccg caaatcctga tccttgatga ggcaaccgct agcattgact
    20221 ccgggactga acaggcgatt caacatgctc tggcggtggt gcgtgaacat actacgcttg
    20281 tggtgattgc tcaccgctta tcgactattg ttgatgccga caccattctg gtgcttcatc
    20341 gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag ggacgatact
    20401 ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg cgtgaagagg
    20461 aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg taacgcactg
    20521 tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct ctttctcgtt
    20581 tttcattttt ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca ttaccgaatt
    20641 ctgaccggag gggatctatg aagctggtga ccgtgattat caaaccattc aaacttgaag
    20701 acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc gaagtgaaag
    20761 gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac agcgtcaact
    20821 tcctgccaaa agtaaaaatt gatgtggcga ttgctgatga ccaactcgat gaagtgatcg
    20881 atatcgtcag caaagcggct tacaccggaa aaattggcga cggcaaaatc ttcgtcgctg
    20941 aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg ctgtaatctc
    21001 tggcacacag caacaggaac gaaaaatgaa gatagcaacg ataaaaactg ggcttacttc
    21061 actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata aagccgacaa
    21121 tgcgtttatg atgatttgta ctgcgctggt gctgtttatg accgttccgg ggattgccct
    21181 gttttatgga gggctgattc gtggcaaaaa cgtgctgtcg atgctgacgc aggtgaccgt
    21241 gacgtttgca ctggtctgta ttctctgggt ggtttacggc tactcgctgg cgtttggcga
    21301 aggcaacaac ttcttcggca acattaacgg gttgatgctg aaaaacattg aactgacggc
    21361 ggtgatgggc agcatttatc agtatatcca cgtggcgttt cagggatcgt ttgcctgcat
    21421 taccgtaggc ttgatagttg gggcgctggc ggaacgaatc cgcttctcag ctgtgttgat
    21481 ttttgtggtg gtatggctga cgctctccta cattccgatt gcgcatatgg tctggggcgg
    21541 cggtttgctg gcttctcacg gggcactgga tttcgcgggt ggcacggtgg tgcacattaa
    21601 cgccgcaatc gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg gcttcggtaa
    21661 agaggcgttt aaaccgcaca acctgccgat ggtcttcacc gggactgcta ttctctatat
    21721 cggttggttt ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg cggcactggc
    21781 atttgtgaat actgtggtcg caacggcggc agcaattctt ggctggatct tcggtgaatg
    21841 ggcgctgcgc ggtaagcctt cactgctggg agcgtgttct ggcgcgattg ccggtctggt
    21901 cggcgtgacg ccagcgtgtg gctacattgg tgttggcggc gcgttgatta tcggcgtggt
    21961 agctggtctg gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc gggtggatga
    22021 tccctgcgat gtcttcggtg tgcacggtgt ttgtggcatt gtcggctgta tcatgaccgg
    22081 gatttttgcc gccagttcgc tgggcggcgt gggcttcgct gaaggtgtga cgatgggcca
    22141 tcagttgctg gtacagctgg aaagcatcgc cattacgatt gtctggtctg gtgtggtggc
    22201 atttatcggc tacaaactgg cggatttgac ggttggtctg cgtgtaccgg aagagcagga
    22261 gcgagaaggg ctggacgtca acagccacgg cgagaatgcc tataacgcgt aacaagcact
    22321 gcaaaaaaca gccggacggt tttcacctcc ggctattttt ttaattgtga ttacgcatca
    22381 ccccttcctg aacggtcgag gcaaccagca cgccgtcctg ggtataaaac tcaccgcgca
    22441 caaagccgcg tgcgctggac gccgaggtgc tttccacgct atacagcagc cattcattca
    22501 aattaaacgg gcgatggaac cacatggaat ggtcaatggt ggcaatctga atccccggtt
    22561 cgagaaaacc gatgccgtgc ggctgtagag ctaccggcag gaaattaaga tcagaagcat
    22621 aaccgagcag atactgatga acgcgcagat catccggcac gctaccattt gcgcggatcc
    22681 acacctgacg atgcggttct gcgacgtgac ctttcagtgg gttatgaaac tccaccggac
    22741 ggacttccag cggacgatcg cagatgaatt tatctttcag cactggcggc agcaggtgcg
    22801 ccagcgattg ggcgatttgc gtttctgaag gcaggccatc aggcgctggc gcggacggca
    22861 ttgttttttg atgttcgaaa cccgcttctg gtgcctggaa agaggcagtc atataaaaaa
    22921 tcggtttgcc gttttgaata gcagcaaccc ggcgggcgct gaagctgtta ccgtcacgca
    22981 gcgtttcgac atcataaata atcggcttct tactatcgcc agggcgaaga aagtagctgt
    23041 gaaaagaatg taccagccgc tcgtcaggga cggtctcttt tgcagcatac aaggcctgac
    23101 ccacgacctg gccgccaaat acctggcgta aacctaaatc ttcactctgg ccgcgaaaga
    23161 gtccttcctc aattttttcc agatttaaca atgtcagtaa attttttagc gcctgactca
    23221 tataactctc cagtaacaaa gctgccgcag caagccaaag tgagttgagt ataacgcaaa
    23281 tttgccactg gtccgatggg tgcaatggtc tgaattacgg gctaattaca ggcagaaatg
    23341 cgtgatgtgt gccacacttg ttgacgttac tattttgtta accactcttc cggcgaggaa
    23401 agttagcccg ctggtgcatt gataataagg agaagtgaat gaaactcgtg cacatggcca
    23461 gtggtttagc ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg gatattcaga
    23521 cgccagcacc ggctgcaaat acgtctattt cagcaacaca acaacctgct atccagcaac
    23581 cgaatgtctc cggtaccgtt tggatccgtc agaaagtcgc actgccgcct gatgccgtgc
    23641 tgaccgtgac actttctgac gcgtcgttag ccgatgcgcc gtcgaaagtg ctggcgcaga
    23701 aagcggtgcg taccgaaggt aaacagtcac cattcagctt tgttctgcca tttaacccgg
    23761 cagatgttca gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg aatgacaaac
    23821 tggtatttat taccgatacc gttcagccgg tgatcaacca gggcggaact aaagccgacc
    23881 tgacattggt gccggtgcag caaaccgccg tgccggttca ggctagcggt ggcgcaacga
    23941 ctaccgtacc ttcaacttca ccaacccagg tgaatccgtc ttcggcagtt ccagctccaa
    24001 cgcaatatta agcgaagtta accctctccg cccggagagg gttagtagtt ccagcgataa
    24061 agctgcaagt cgatttgccc gcttcccgac accatcacac cttctgccag taatgcctgt
    24121 cgctgacgct gtaaatccgg tccggtcagt gaaatggtgc cgtggcgatt aaccacccgg
    24181 tgccagggca aggtgctgcc ttcggggaga cgctttaaca caccgcctac ctggcgcgcg
    24241 gcgcgcggcg atcccgccag tttcgccaca tcaccgtaag tggtgacata gccttcggga
    24301 atagcggcta cgatttgcca gacgcgttgg ggaaatgaat cttctttttc catcttttct
    24361 tcctgaggta atttttcagc ataatctgga aaaacgcccg agtgaagccg cattgcgcaa
    24421 gaaaccagca tctggcacgc aatgggttgc aattagccgg ggcagcagtg ataatgcgcc
    24481 tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg gttctcccgc aacgctactc
    24541 tgtttaccag gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg tgccgggatg
    24601 tagctggcag ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa atcccaacat
    24661 ggctaaactt taaccacaac tgacgtcgca agaattgtct ggctgcgcag tacgcttcgg
    24721 aggtatgtct gatgaagtat gttgatggtt ttgttgttgc cgttcctgcc gataaaaagg
    24781 atgcctatcg ggaaatggcc gctaaggccg cgccattatt taaagagttc ggtgcgcttc
    24841 gtattgtcga atgctgggcc agcgatgtac cggatggtaa agtgaccgat tttcgtatgg
    24901 cggtgaaagc ggaagagaat gaagaggttg tctttagctg gattgaatac ccttcaaaag
    24961 aggtccgcga cgctgctaat caaaagatga tgtcggaccc acggatgaaa gagttcggcg
    25021 agtccatgcc gtttgacggc aagcgaatga tctatggtgg attcgaatca atcatcgacg
    25081 agtaggtagc gtgacgggct gtgcggcgtg ccgtcagccc taaaattcac aaatgttgct
    25141 cggcccagcg taagaaatcc tctttcggta atgccttgct gtagagccag ccctgaccgt
    25201 agtgcacgcc atgctggcgt agccactctt cttgtttact ggtttcgatt ccctccgcaa
    25261 ccattttcag tttcagtgtt tttgccattt cgatgatatg cggcgtgaca tttttatatt
    25321 ccagcgcatc aacgaaagat ttatcgatct tcagaatgtc gacatccaga tcctgtaaat
    25381 agcttaaact tgaataccct gtaccaaaat catcgagata aatttcatgg cccgcctccc
    25441 ggtagcgaga aattatcggg gcgctggttt tcggatcggc aaactcgcgt tcagtgagtt
    25501 caagcgcgat ctgtctggga ttaacctgat agtgattgat catttcacgc agcaattgcg
    25561 ggattttttc cgaggtgagc acggtggatt caagattgat cgaaatatgc tgctgaggat
    25621 gctgacgcag ccagtcgccc atatcttcaa agacgcttct tataatcagt agcgtcaatg
    25681 gctcagaaag gcccgtttgc tgtgccagcg gaataaaact atctggtgac aaccaactac
    25741 cgtctgtctg cggccagcgc gccagtgcct cagcaccgac aattttgcca ttggctaagg
    25801 agacaatcgg ctgatagtgc acgcaaatat cacgattttc gatagcatcc tgcagccgat
    25861 gatgcggtga ttggatacgg cgcaaaatac gcagcacaaa catcgctgcc agcaggccaa
    25921 tcaccaaccc gagcggtaac caaataaaga cttgccgatg ccagcctttc tgcaacattt
    25981 tcgttgaagc ccaggtgatg atcgaaatat tcatctccgg taagggctgg atatcgtaaa
    26041 tgattccatt attttcgata tgctcaccgg gtgttttttg tagcctggta ataattccct
    26101 gagcaagttc atcgctgctg gttatgacaa cgttatgggc attgccaata atggcggcat
    26161 caatttgcca tgagctatag ggaataacat caataaagga ggcagggtcg atcatgacga
    26221 cataatgtgc cgttcccatg gcgaccatgt aacggataat gcctaaatcg ctatgcgatg
    26281 ttaaccagac acgataacca tctttcgaaa ttttacctgg ctcggggaag gtatcgggcg
    26341 ggctttcatg ctccagagac gaacactggg gaacgttgtt atcgatataa accacttcct
    26401 gaatatagcg ataactgtaa gatacccgac gcatttccat gagatgggct tcgctacagg
    26461 cagcgccttg ccatctttcc agctcttgca gcgcatcttt cccttgtgtc gccaccttat
    26521 tggcccgaat agcgacgcgg gaggaatacg tattcagctc ttcaataaat gatgtttcta
    26581 cctgctgatg ggccagccag atgcttaagc cgacaggcag caatactgaa agaatcagaa
    26641 ctcccgaaat aaggctgacc agatgtcgtg ttctcaccag catatcctta ttaacacagt
    26701 gtagggaaag tatatctgat tgtggaaaaa ggctggcagg agataaggaa gtagggctgg
    26761 tatttcgtga agtgatgggt ggaaacgatc gttgtcattt gaccaggcgt agagctttac
    26821 gcctggtatg taaccctaac ttaagatgtt gattcggcgc gagcgagggt aaaaatatcg
    26881 taaaaagata gttcaccttt acggacgatc attttttgca gttgttcttt atgaacatcc
    26941 gcgacacgcg gagagttcag gatggcttca accagtgcgg caggtacaaa gcgcgtgttg
    27001 taccattcgc cgttatagca aacgcggaaa tccagcacgt cgatatcttc atagcggtaa
    27061 cgtgggtaga catcgaaaaa gaagaaacca ttaagcagga taaataatac aaccagtagc
    27121 cagacagagc ctgaaagcgt ttcggattgc aacatcaccg ccagggtagc aaaaaaagca
    27181 acgtacatac cgataaacag ccccggatgt ttacggataa aactgatact aaagcggggt
    27241 ttgttgtcgc gtttttcgcg ggtattcaga gactcaatcg tttctgtcag caggcgttgt
    27301 atttcggtca tcgtttgcct tcgtgatatc tgcaactttc cagggaacac cctattttaa
    27361 cgtgggaata gtagagggga agtaacagat ttgccacgat tttgcataac agttgagaaa
    27421 agctggttac aattttttaa tgattctggc tggattaccg cccacgacaa cgttgtccgg
    27481 gacatctttt gtgacaactg cacctgaggc taccacgacg ttatcaccaa tggtcacacc
    27541 agggttaatg actgcacgtc cgccaatcca gacgttatta ccgatggtga cgggtttccc
    27601 cagttcagca ccgctattac gtgctacagg gtcgatggga tgtgttgccg tgtagatatg
    27661 aacgcctggt gccaacatac agttatcacc aatgcgaata gggcagacat caagcatcac
    27721 gcaatcgaag ttggcgaaaa aattattgcc gagaaaaatg ttatagccat agtcacagcg
    27781 aaacgttggc tcaatataag cctctgtcac ctgaccgaat aaatcagcaa gaatttgctg
    27841 gcgtaatgtg tgctcttccg ccagggaatg attgtatcgg tgaataagcc gacgagctcg
    27901 caggcgatcg cgagataacg cctcatctgc cgagtgatac aactcaccag caatcatctt
    27961 ttctttttct gtgctcatta ctgacttccg gttgctgaaa atgcggcaac acagtaagac
    28021 gaaaagtcag tgaagagaac gttcccatta catctgtgtg atgaagatca catagggtat
    28081 gtcttcatgg cgaaaaaagt gcaaaattct taataaacag ccagttatag ctccgaaagt
    28141 gagttattta ttagcgaata aatttccata ctgaggaagg gatcttgtcg tacagtttat
    28201 tcatggtcaa ttcggcgagg cggtgatctg cggctgagta aaataccgcc agttcattat
    28261 ctgataattc gtatttattt ttctcgataa cacgctccag cgtgtcaatt gtctggcaac
    28321 gacgtaaacg cattaaataa tcggttttcg ttaaaggttt ttcggacata cttctaccca
    28381 tggttgtaat aattctaaca agataaactc gcaggattct ctttcgtcgc attgacaaaa
    28441 caacggaata gtcgattacc tgacttccgc catttctgaa gatcctgcat attaatacca
    28501 taactactga acaacataaa ggtgtcatcc agatattcgt cgatctgctc aatgagctta
    28561 ttgtcttcat tatacttaat tttgtaatta agtgcgaagg tcgcaatatg ctcaatcagt
    28621 tcattcaact ggaggttgat cgccgaggtt gggtcgttaa cccagccatg attgctttct
    28681 tcaaggtttg caaggcagtc atgatacagg gtttcacaga gaaacttaag ctgtgcgata
    28741 tcatgtcttt tgggtgagta ttcatccata acgcgtcccc ttcttagcgg ttgaactaac
    28801 ggacaccttt cgggatggaa aaaacttact gacctggact tgccctctcg tttgttagct
    28861 taactataag ccactctttg taggttttca tcgcattatt acgaaaaatt acaattagga
    28921 aggaagcggg acaggactca ctttttcata ctagcaccaa cccgcagcag gtaaaagcag
    28981 tttgctcgaa ctgttcataa catttcagaa ttatcgccag aagaaattgg tgacactaat
    29041 accaggattg ctctgaatat gacgtaataa ccgaggaatg aataaagaat taccgcaaat
    29101 aa
//
LOCUS       DAZFRE010000067        28998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFRE010000067 DAZFRE010000000
VERSION     DAZFRE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.067"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHKUI_003220"
     CDS             <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHKUI_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO6599369.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEFRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
     gene            705..3323
                     /gene="bcsA"
                     /locus_tag="ACHKUI_003221"
                     /pseudo
     CDS             705..3323
                     /gene="bcsA"
                     /locus_tag="ACHKUI_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHKUI_003222"
     CDS             3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHKUI_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6599370.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPSPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHKUI_003223"
     CDS             5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHKUI_003223"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6599371.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVPTGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHKUI_003224"
     CDS             6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHKUI_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO6599372.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVVNLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHKUI_003225"
     CDS             10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHKUI_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6599373.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12494..13780
                     /gene="dctA"
                     /locus_tag="ACHKUI_003226"
     CDS             12494..13780
                     /gene="dctA"
                     /locus_tag="ACHKUI_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6599374.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14000..15496
                     /gene="yhjJ"
                     /locus_tag="ACHKUI_003227"
     CDS             14000..15496
                     /gene="yhjJ"
                     /locus_tag="ACHKUI_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6599375.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15592..16521)
                     /gene="kdgK"
                     /locus_tag="ACHKUI_003228"
     CDS             complement(15592..16521)
                     /gene="kdgK"
                     /locus_tag="ACHKUI_003228"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6599376.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGIAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16753..17520
                     /gene="pdeH"
                     /locus_tag="ACHKUI_003229"
     CDS             16753..17520
                     /gene="pdeH"
                     /locus_tag="ACHKUI_003229"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6599377.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17590..19650
                     /gene="yhjG"
                     /locus_tag="ACHKUI_003230"
     CDS             17590..19650
                     /gene="yhjG"
                     /locus_tag="ACHKUI_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6599378.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVIGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQVFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19829..21151)
                     /gene="yhjE"
                     /locus_tag="ACHKUI_003231"
     CDS             complement(19829..21151)
                     /gene="yhjE"
                     /locus_tag="ACHKUI_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599379.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21544..22557)
                     /gene="yhjD"
                     /locus_tag="ACHKUI_003232"
     CDS             complement(21544..22557)
                     /gene="yhjD"
                     /locus_tag="ACHKUI_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6599380.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22606..23505)
                     /gene="rcdB"
                     /locus_tag="ACHKUI_003233"
     CDS             complement(22606..23505)
                     /gene="rcdB"
                     /locus_tag="ACHKUI_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6599381.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGIAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24025..24627
                     /gene="yhjB"
                     /locus_tag="ACHKUI_003234"
     CDS             24025..24627
                     /gene="yhjB"
                     /locus_tag="ACHKUI_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6599382.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24678..26327)
                     /gene="treF"
                     /locus_tag="ACHKUI_003235"
     CDS             complement(24678..26327)
                     /gene="treF"
                     /locus_tag="ACHKUI_003235"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6599383.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTHEPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26732..28129
                     /gene="ccp"
                     /locus_tag="ACHKUI_003236"
     CDS             26732..28129
                     /gene="ccp"
                     /locus_tag="ACHKUI_003236"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6599384.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKLSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            28340..>28998
                     /locus_tag="ACHKUI_003237"
     CDS             28340..>28998
                     /locus_tag="ACHKUI_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6599385.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMCPGQLFMDPKGMIEACDENTIGVVPTFGVTYTGNYE
                     FP"
BASE COUNT         6871 a         7285 c         7982 g         6860 t
ORIGIN      
        1 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg
       61 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta acgttgattt tacccaccgt
      121 cagggttggg caagagctat gctggatggc caggactggc gtgacgccgg gttgcgctat
      181 acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca
      241 cagcactggc aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc
      301 gggcgttacc agtggatttt aattgactta ccgcgtgatg cctcgcagat aacccaccag
      361 ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc
      421 cgactgcatc agcaagcgct gccggatggt gcacatattt tgattaatga cttccgtatt
      481 ggcagccagg ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattgctg
      541 ccgatgctca ttcatcgtga tgaagcgatg gctgaatgtc tggcagctaa gcaaccagtc
      601 ggtgaatttc gcagtgatgc gctggcggct gaagaaatac tgacgctggc gaactggtgc
      661 ctgttgaact attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc
      721 ggtggttgct tatcccgccg gtcaacgcac ggcttattgg gcgttatcgc gattatcgtc
      781 gtcacggcgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga
      841 tttttattcc gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt
      901 atccgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat cttattcaaa
      961 catgctggtt actgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat
     1021 tttcaggtct acaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
     1081 gcgttagcca taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg
     1141 gggcgcggcg gttgatcctt ggtatcatcg tcaccttctc gctgattctg gcgttaatct
     1201 gcgttactca gccgtttaac ccgctagcgc agtttatctt cctgatgctg ctgtgggggg
     1261 tagcgctgat cgtacggcgg atgccggggc gcttctcggc gctgatgctg attgtgctgt
     1321 cgctgaccgt ttcttgccgt tatatctggt ggcgttacac ctcgacgtta aactgggacg
     1381 atccggtcag cctggtgtgc gggcttattc tgctgttcgc cgaaacgtac gcgtggattg
     1441 tgctggtgct cggctatttc caggtcgtat ggcctctgaa tcgttagccg gtgccattgc
     1501 cgaaagatat gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc
     1561 tcaacgtggt gaaaaatacc atttacgctt cgctgggtat cgactggccg aaagacaagc
     1621 tgaacatctg gatcctcgat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg
     1681 tgggggtgaa atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca
     1741 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatatt cgactgcgac cacgtaccaa
     1801 cgcgatcgtt cctgcaaatg accatgggat ggttcctgaa agagaaacag ctggcgatga
     1861 tgcagacgcc gcaccacttc ttctcgccgg atccgtttga acgcaacctg gggcgtttcc
     1921 gtaaaacacc gaacgaaggc acgctgttct atggtctggt gcaggacggc aatgatatgt
     1981 gggatgccac tttcttctgc ggctcatgtg cggtgattcg ccgtaagccg ctggatgaga
     2041 ttggcggtat tgctgtcgaa acggtaaccg aagatgcgca cacttctctg cgtctgcacc
     2101 gtcgtggcta cacctccgcg tatatgcgta ttccgcaggc agcggggctg gcgaccgaaa
     2161 gtctctcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc
     2221 gtctcgataa cccgctcacc ggtaaagggc tgaagtttgc tcagcgactg tgctatgtca
     2281 acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg
     2341 cgttcctgct gtttcatgcc tacatcatct acgcgccagc gttgatgatc gccctgtttg
     2401 tgctaccgca tatgatccat gccagcctga ctaactcgaa gatccagggc aaatatcgcc
     2461 actctttctg gagtgaaatc tacgaaacag tgctggcgtg gtatatcgca ccaccgacgc
     2521 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
     2581 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca
     2641 acctggtggg cgtagcggtt gggatctggc gctacttcta tggtccgcca accgagatgc
     2701 tcaccgtggt cgtcagtatg gtgtgggtat tctacaacct gattattctc ggcggcgcag
     2761 tcgcagtatc ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc
     2821 ctgcagcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
     2881 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
     2941 atctgttgct taaacgcggt cagcaggaat acgtcttccc ggctcaggtg gcgcgcgtga
     3001 tgggtaatga agttgggctg aaattaatgc cgctcactac ccagcaacat atcgattttg
     3061 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag
     3121 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
     3181 tggcggaatt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
     3241 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
     3301 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaaatatt ctggatttgt
     3361 gcagtggctc tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
     3421 caaccactga tcaatgctga gccagctgta gccgcccagg cggagcaaaa tccgcaggtg
     3481 gggcaagtga tgccgggcgt gcagggcgca gatgcgccag tcgtggcgca gaacggtcct
     3541 tcccgtgatg tgaagctgac ctttgcgcaa attgcgccgt cgccgggcag catggtgcta
     3601 cgtggcatta acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg
     3661 aaggcgatgc tcaacctcga atacacgcca tcgccatcat tactgcctgt ccagtcgcag
     3721 ttaaaggttt atctcaatga tgaactgatg ggcgtgctgc cagtgaccaa agaacagttg
     3781 ggtaaaaaaa cgctggcaca aatgcccatt aacccgctgt ttattactga tttcaaccgt
     3841 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
     3901 ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg
     3961 aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgtgataa ccgcaccaac
     4021 accttgccga tggtctttgc gggtgcgccg gatgttgagc tgcaacaagc atctgccatt
     4081 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
     4141 aaccaactgc cggatcgcaa tgccattgtc tttgccacca atgacaaacg cccggacttc
     4201 ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat
     4261 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacttgtt gcaggcagcg
     4321 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gtgtggtagt gaatgaagtg
     4381 aaaccgctgc taccgcgtaa accgtacgat gcgccgaact gggtgcgtac cgatcgtccg
     4441 gtcacctttg gtgaactgaa aacctatgaa gaacaattac aatccagcgg tcttgagcca
     4501 gcagcgatta acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc
     4561 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
     4621 gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg
     4681 aaccgcctgc tgctgcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc
     4741 tctattccgg cgctgaaact gggcgcgacc aaccagctgc ggttcgactt tgagtatatg
     4801 aacccgatgc cgggcggttc ggtggataac tgcattacct tccagccggt gcagaatcat
     4861 gtggtgattg gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg
     4921 ccggatctac gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatttgtcg
     4981 caaaccatca ccgtgatgcc gaaaacgcca aacgaagcac agatggaaac cttgctgaat
     5041 accgttggct ttatcggtgc gcagacgggc ttcccggcga ttaacctgac ggtgaccgat
     5101 gatggcagca ccattcaggg caaagatgcc gacatcatga tcgtcggtgg tatcccggac
     5161 aaactgaaag acgataagca gatcgacctg ttggtgcagg ctaccgaaag ctgggtgaaa
     5221 acgccgatgc gccagacccc gttccccggt attgtgccgg acgagagcga tcgcgcggca
     5281 gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg tttccagtcg
     5341 ccgtataacg accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa
     5401 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
     5461 gtgatccgcg agtccggtat caacagccta cgtgttggcg atgtttatta cgtaggtcat
     5521 ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
     5581 ctggcggcta tcagtgtgat attgctggcg tgggtactgt ggcgtctgct gcgtattatt
     5641 agtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
     5701 ctcgtgacga tgctgctgct ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
     5761 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
     5821 gcgcgcaaaa tcaccacctc cgaagggcaa agttacggca tgttctttgc cctggcggct
     5881 aacgaccgtg cagctttcga taatcttctc gactggacgc agaacaatct cgctcagggt
     5941 tctttaaaag aacatttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
     6001 gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcctggtc gttgctggag
     6061 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcatt gctaaaacgt
     6121 atcgcgcggg aggaagtggt gacggtgcct gggctgggct ccatgttgtt accgggcaaa
     6181 gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacctgcc gccgacgctg
     6241 gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacgt
     6301 ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta tgagaaagac
     6361 aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt
     6421 tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg
     6481 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
     6541 cctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcccttt
     6601 ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg tggccgataa ctttcccggc
     6661 agcgatgcct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
     6721 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
     6781 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
     6841 caaccgctca gcaacagttg ctggagcaag ttcggttagg cgaagcgacc catcgtgaag
     6901 acctggtgca acagtcgttg tatcggctgg aacttattga tccgaataac ccggatgtga
     6961 ttgctgcccg ttttcgttct ctgttacgtc agggcgatat tgatggcgca caaaaacagc
     7021 tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta taaatcgtcg cggaccacaa
     7081 tgttactgtc tacgccggat ggtcgtcagg cactgcaaca ggctcgattg caggcgacta
     7141 ccggtcatgc ggaagaggcc gtggcgagtt acaacaagct gttcaacggt gcgccgccgg
     7201 aaggtgacat tgctgtcgag tactggagta cggtggcgaa aattccggct cgccgtggtg
     7261 aagcgattaa tcagctaaaa cgcattaatg cggatacgcc gggcaatacg ggcctgcaaa
     7321 acaatctggc gctattactg tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg
     7381 aacagatggc gaaatcgaac gccgggcgcg aaggggcctc taaaatctgg tacgggcaga
     7441 ttaaagacat gcccgtcagc gatgccagcg ttcaggcact gaaaaaatat ctctcgatct
     7501 ttagcgatgg cgatagcgtg gctgctgcac aatcgcaact ggcagaacag caaaaacagc
     7561 tggccgaccc tgctttccgc gctcgtgcgc aaggtttagc ggcggtggac tctggcatgg
     7621 cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc gaacccgaaa gacagtgagg
     7681 ctctgggggc gctgggccag gcgtattccc agaaaggcga tcgcgccaat gcagtggtga
     7741 atctggaaaa agccctcgcg ctggatccgc acaacagcaa taacgacaaa tggaacagcc
     7801 tgctgaaagt aaaccgctac tggctggcga tccagcaggg cgatgccgcg ctgaaagcca
     7861 ataatcctga ccgggcagaa cggctgttcc agcaggcacg taatgtcgat aacaccgaca
     7921 gctatgcagt gcttgggctg ggcgatgtgg cgatggcgcg caaagattat cccgccgccg
     7981 aacgctatta ccagcagacc ttgcgtatgg acagcggcaa cactaacgcc gtgcgcgggc
     8041 tggcgaatat ttaccgccag cagtcgccag aaaaagccga agcgtatatc gcctcgctct
     8101 ctgccagcca gcggcgcagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg
     8161 cacagcaggc agaggcactg gaaaaccagg ggaaatgggc gcaggcggca gcacttcagc
     8221 ggcaacgact ggcgctggac cccggcagtg tatggattac ttaccgactt tcgcaggatc
     8281 tctggcaagc cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga
     8341 agccgaacga cccggagcag gtttacgctt acgggctgta cctctccggt cacgaccagg
     8401 acagagcggc gctggcgcat atcaacagcc tgccgcgcgg gcagtggaac agcaatattc
     8461 aggagctggt taatcgactg caaaacgatc aggtgctgga aaccgctaat cgcctgcgag
     8521 aaaacggtaa agaggcagaa gcggaagcga tgctgcgcca gcaaccgcct tcctcgcgta
     8581 ttgacctcac gctggctgac tgggcgcagc agcgacgtga ttacaccgcc gcccgcgctg
     8641 catatcagaa tgtcctgacg cgggagccaa ctaacgccga tgccattctt ggtctgacgg
     8701 aagtggatat tgctgccggt gacaaagcgg cggcacgtag ccagctggcg aaactgcctg
     8761 ccactgataa cgcctcgctg aacacacagc ggcgcgtggc gctggcacag gcgcagcttg
     8821 gcgataccgc agcggcgcag cagacattta ataagttgat cccgcaggca aaatctcagc
     8881 caccgtcgat ggaaagcgcg atggtactgc gtgatggtgc gaagtttgaa gcgcaggcgg
     8941 gcgatccaac gcaggcgctg gaaacctaca aagacgccat ggttgcatcc ggtgtgacca
     9001 cgacgcgtcc gcaggataac gacactttta cccggctgac ccgtaacgac gagaaagatg
     9061 actggctgaa acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta
     9121 acgtcactct cgagcacgat tactggggtt cgagcggcac cggtggttac tccgatctga
     9181 aagcgcacac cactatgttg caggtggacg cgccctattc tgacgggcgg atgttctttc
     9241 gcagtgattt cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg
     9301 atgacaactg gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt
     9361 ccggtgccag cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggtacca
     9421 cgccgatggg cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg
     9481 ggccgctggg ttacaccgtt aacgcccacc gtcggcctat ctccagttct ttgctggcct
     9541 ttggtgggca aaaagactct ccgagcaata ccgggaaaaa atggggcggc gtgcgtgccg
     9601 acggcgtggg gctaagtctg agctacgata aaggtgaagc aaacggcgtc tgggcatcgc
     9661 ttagcggtga tcagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga
     9721 cgggctatta ctataaggtc atcaaccaga acaatcgccg cgtcacaatc ggcctgaaca
     9781 acatgatctg gcattatgac aaagacctga gtggctactc actcggtcag ggcggttact
     9841 acagtccgca ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa
     9901 actggtcgtg ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc
     9961 cgcgttatcc gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat
    10021 ccaacgatgg cggcagcagt cagggctttg gctacacggc gcgggcatta cttgaacgac
    10081 gtgttacttc caactggttt gtcggcacgg caattgatat ccagcaggca aaagattacg
    10141 cccccagcca tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg
    10201 atttaccgcc gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct
    10261 cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca
    10321 atttgcgcgt aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc
    10381 tggtgttcgt ttttattttt tgcaccgttt tgctgttcca tctggtccag cagaatcgct
    10441 ataacacggc tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttctg
    10501 ccattttgaa aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag
    10561 gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt
    10621 ttattccaga gcgtccggtg ccggtaatgg ttactcgcct gtttgagcta ccggtgcaaa
    10681 tctcgctggg cgtttactcg ctggaacgtc cggcaaaccc gcagccaatt gcctatctgg
    10741 tgttacaggc ggattccttc cgcatgtata agttcgtgat gagcaccctc tcaacgttag
    10801 tgaccattta cttactcttg tcgttaatat tgacggtggc gattagctgg tgcattaacc
    10861 gcctgatttt gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccaccccagg
    10921 agcttgttgg tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt
    10981 tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg
    11041 aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc
    11101 tggagcaggt ggtggcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc
    11161 tgcgtgatac tgcgggtgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg
    11221 tggaaaaact caaatcggta ctgtcgccac gcatgatcct cgcgcagatt agcggttatg
    11281 actttgctgt cattgccaac ggtgtacagg aaccgtggca cgctatcaca ttaggtcagc
    11341 aagtgctcac tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact
    11401 gtagcattgg cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg
    11461 ctatttctgc ggcatttacc gctcgccata aaggcaaaaa tcagattcag ttctttgatc
    11521 cgcagcagat ggaagccgcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac
    11581 tggaaaatca tcagtttgcg atttggttac agccacaggt cgagatgacc agcggtaaac
    11641 tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgcccg
    11701 atggcttaat cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc
    11761 tggaagagtc ctgtcgattg cttgctgcct ggcaagagcg cggcattatg ctgcccttgt
    11821 cggtaaacct ctcagcgctg caactgatgc atccaaatat ggtggcggat atgctggaat
    11881 tgttaacccg ctatcgtatt cagccgggaa cactgattct ggaagtgaca gaaagccgac
    11941 gtattgacga tcctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc
    12001 gggtggcgct ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga
    12061 aatcgttgcc aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggt ttgccggaag
    12121 atagcagcat gattgctgca attatcatgc tggcgcagag cctgaaccta caaatgattg
    12181 ccgaaggcgt ggagactgaa gcacaacgcg actggctggc aaaagcgggc gttggtattg
    12241 cccagggctt cctttttgct cgcccactcc ctattgaaat cttcgaagag agttacctgg
    12301 aagaaaagta gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc
    12361 cagctcaaac tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt
    12421 gtctgggtgt tattttaagg ccgcaggtac cccataacct tacaagacct gtggttttac
    12481 taaaggacac cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc
    12541 gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc
    12601 gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac
    12661 cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt
    12721 cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt
    12781 taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc
    12841 ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt
    12901 catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt
    12961 tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa
    13021 cgtgatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc
    13081 acctattggt gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact
    13141 ggtgcaactg gggcagctga ttatctgctt ctacatcacc tgtatcctgt ttgtggtgct
    13201 ggtactgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgttacat
    13261 ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat
    13321 gctcgacaag atggagaaac tcggctgccg caaatcggtg gtggggctgg tcatcccgac
    13381 aggctactcg tttaatcttg atggcacatc gatatacctg acaatggcgg cggtgtttat
    13441 cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt
    13501 gctgctttct tctaaagggg cggcaggggt aacgggtagt ggttttatcg tgctggcggc
    13561 gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga
    13621 ccgctttatg tcagaagctc gtgcgctgac taacctggtc ggtaacggcg tagcgaccat
    13681 tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa
    13741 tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcaccact tatgcccgca
    13801 ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc
    13861 tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac
    13921 acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt attaccagga
    13981 tagttgatca ggggttcaca tgcagggcac aaaaattcga cttttagcgg gcggtttgct
    14041 gatgatggcc actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca
    14101 acaggggacg ctttccaacg gtttacagtg gcaagtgctg actacgcccc agcgtcccag
    14161 cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca
    14221 gagcggttac agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc
    14281 agcgcaggcg cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt
    14341 aattgtctct tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgactt
    14401 gctgaaagag gcgctctctt atctggcaaa tgccactggc aaactgacta tcacgccaga
    14461 aaccatcaac catgcgttgc aaagtcagga catggtggca acctggcctg ccgatactaa
    14521 agagggctgg tggcgttatc gtctgaaagg atcaaccttg ttaggtcacg atcctgccga
    14581 tccgctgaaa caacccgttg aagcggaaaa gattaaagat ttctatcaga aatggtacac
    14641 cccggatgca atgacgctgc tggtggtggg aaacgtggat gcgcgctcgg tcgtcgacca
    14701 aatcaataaa acgtttggcg aactgaaagg caaacgtgaa acaccggctc cggtgccgac
    14761 gctttctccg ctgcgtgcgg aagcggtgag tattatgacc gacgcggtgc gtcaggaccg
    14821 gctatctatc atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg
    14881 ctactggcgt gcggacctgg cccgtgaggc gctgttctgg catgttcagc aagcgttaag
    14941 cgccagtaac agtaaagaca tcggtcttgg atttgactgt cgtgtgctgt atctgcgtgc
    15001 gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt
    15061 ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt
    15121 agtggcgcaa aagaaactgg agctgcagaa actgtttgcc gcctatgcgc gagctgatac
    15181 cgatattctg atggggcagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc
    15241 ggagcaatat cagaaactgc gtcaggattt ccttaacagc ctgacggtgg agatgttaaa
    15301 tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg
    15361 cgagccggaa tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc
    15421 gactgcggct gccgccacat ctgtcgccac ggatgacgta catcctgaag tgacggatat
    15481 tccacctgta cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga
    15541 tgagctggat aggggggtaa cactctatcc agccttttgc atatgctgct tttacgctgg
    15601 catcgcttca cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg
    15661 cccgcgtttc gctgcgtctt ccgcaattcc gcccgtcaaa cgtaccgcca gataaccggc
    15721 actgaaagag tcgccagctg cggtggtatc gatcactttt tctttcggca gttttaccgc
    15781 cggaacatcc actaaccctt cgccagcaat ggacaccagg caagaatccg ccccgcgttt
    15841 caccaccact tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg
    15901 ttgaccccac agcgcgtctt catcgtccag cgtcaggaag gcgatatccg tgcattccag
    15961 catttgttgg tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt
    16021 attgtcgaaa atcacttttc cgccgttggc gcggcattcg cgcagcaggg aaagcagctt
    16081 ttcgcggctg gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa
    16141 attcgccagc tcttcgcaaa tcgccgcgga ttgctcactc tccagccaga atttggcggc
    16201 ggcttcgtta cgccagtagt agaacgtacg ttcgccggtg ctgtcggttt caatgtagta
    16261 aaggcccggc agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgcg
    16321 ccaggcgtcc agcatctgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac
    16381 ggttaatgcc gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc
    16441 gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat
    16501 cacggcaatc tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg
    16561 cgctgtggtg gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca
    16621 gaacgtattc ctgaaagatt ccgttgtgga gtggggaaat ttaccccgtc caggcggtaa
    16681 agttctgccc ttgcgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac
    16741 aggacatctt tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc
    16801 gagagcttgc aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg
    16861 atctatcaaa catgcgggcg gttaatggcc gtggagctat taacggtggt cacgcatccc
    16921 ttgaaccctt cgcaacgcct gccgccggat cgctatttta ctgaaatcac cgttagccat
    16981 cggatggagg ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttgta
    17041 gagcacggcc tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag
    17101 caaccaaaaa tcctgcgcca gattgaggat cttccctggc tgcgtttcga actggtggaa
    17161 cacatccgcc tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg
    17221 ctggatgatt ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac
    17281 tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc agtcaccgga aggacgcaca
    17341 ctcttttctc agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc
    17401 gtagaaacac cagaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc
    17461 tggtttcttt cacgcccggc accgatagaa acgctgaata cggcggttct ggcgctataa
    17521 gctgcctcat tttccgcctg gctggactat ctttaggact ggcacaggaa gaatatgagg
    17581 aagcgaacga tgagtaaggc aggcaaaata accgctgcga tttcaggggc tttcttgttg
    17641 ttgattgtcg tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc
    17701 atcaaccaga aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg cgatctgggc
    17761 gtggtgtggg agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggccccat
    17821 gtacacgcgg aagacatcat tcttggcaat ccaccggata ttcccgaagt cacaatggtg
    17881 catttgccgc gagtagaagc gacgctggcc ccgctggcgc tgctgaccaa aacggtctgg
    17941 ttgccgtgga tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc cgaaaagaac
    18001 aataactgga cgtttaatct cgccaacgat gacaacaaag acgcgaatgc aaagccgtcg
    18061 gcgtggtcgt ttcagctgga taatattctt ttcgatcaag ggcggatcgc cattgatgat
    18121 aaagtaagca aagcggatct ggaaattttt gtcgatccgt taggcaagcc gctgccgttc
    18181 agcgaagtta tcggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacgttttt
    18241 ggcctgaagg cgcagggacg ttataacggt gaaccgctca ctggtacggg aaaaataggc
    18301 ggtatgctgg cgctgcgtgg cgaaggaacg ccgtttccgg tacaggctga tttccgctct
    18361 ggtaacaccc gtgttgcttt tgatggcgtt gtgaatgacc caatgaagat ggggggtgtc
    18421 gatttacggc ttaaattttc tggcgattcg ctgggtgatc tctatgaact gacgggcgtt
    18481 ctgctgcccg atacaccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact
    18541 gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc gcattggcga tagcgatatc
    18601 cacggttctc tgatctatac caccggcaag ccacgaccaa aactggaagg tgatgtcgag
    18661 tcgcggcaat tgcggctggc ggacctggga ccgttgattg gcgttgattc cgggaaaggg
    18721 gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc
    18781 aaagtgctgc cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt
    18841 cgcttcaaag ggcggcgcat tgagcatggc agcagcctgc cgattagcga tctttctact
    18901 catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catagcgggc
    18961 ggcagcattg cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca
    19021 gatattcagg ctcgtcgact gaaactgaaa gaactgatgc ccgatgtaga actgatgcag
    19081 aagacgctgg gggaaatgaa cggtgacgcg gaactgcgcg gtagcggtaa ctcggtggcg
    19141 gcgcttttag gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc
    19201 cgcaacctga tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt
    19261 ggtgatgatg aggtgcgggt gaactgcgcg gcagcgaatc tggatattgc caacggtgtg
    19321 gcgcgtccgc aggttttcgc ttttgatacc gagaatgcgt tgatcaatgt taccggcacg
    19381 gcaagttttg cttcggaaca gctggattta actatcgatc cagaaagtaa agggattcgc
    19441 attatcacgc tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg
    19501 gtgaaagccg ggccgttgat tgcccgtggt gctgttgcgg cggcactggc aacgctggtt
    19561 acgccagcgg cagcattgct ggcactgatc tcaccttccg aaggggaggc taatcagtgc
    19621 cggacaattt tgtcgcagat gaagaagtga tgataattgc cggatgtctg acatccggca
    19681 attaaacaga tttagattgt tggcaaagtg tgctttgttc atgccggatg cggcgtgaac
    19741 gcctccggct tacaaatcct tgctaattca atatattgta tggaaattct ggcctgataa
    19801 gcgtagcgca tcaggctttt cactctgatt acaacgactg atgtcgcgtc tcatgggtca
    19861 gcagcagggc gattaacgtc aagccagcca tcgccgccag atatagcccc accgcaccta
    19921 acccgtagtt agtctgcaac caggctgcga tatatggcgc aacggaagcc ccgagaatcg
    19981 acgatacgtt gtaagagaac gatgctccgg tgtaacgcac ttctgtcggg aacagctccg
    20041 gcagcagcgc ccccatcgga ccgaaggtca gccccatcag acttaacccc agcagcagga
    20101 aggcaaaaac cagaatcggg ttgccagaac cgagcaaagg gttaaaggcg aatagcgcga
    20161 acaggatgat cagcgtggtg atgattacca tgcttttacg gcgaccaaag gcatcagcca
    20221 gtaatccggc gacgggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca
    20281 acacttcgtt acgcggcagg ccaagcccaa ctggcgcggc ggcggtacta aaggtcatag
    20341 agtagaccgt catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta
    20401 cggttacgcg aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg
    20461 ctttagcgac tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga
    20521 taatgaccag caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct
    20581 cgtcggtcag tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg
    20641 gtgcgcccag ctgcgggaag gagccgtaca atgcacgttt gcgcggtggg gcgttttcag
    20701 tcgccagcag cgccgcgccg ccccattcac cacctaagcc cagaccctga ccaaatcgag
    20761 ccagcgccag cagcagcggg gcgaaaatac caatcgtggc atagcctggc agcagaccaa
    20821 tcaccacggt cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa
    20881 cgcgatcgcc aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg
    20941 cgaaggtggc gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat
    21001 gcggaaacac aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg
    21061 tgccaatgag agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgct
    21121 cgtggtcgag tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat
    21181 agccatatta cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca
    21241 aaaatcatgc aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc
    21301 acataatttt ataaatggtg gattaaactg gttttaggtt aaataaaagt taccaactgg
    21361 atttatatgc tggggttatg agttggaatg aaattgtttg ctcctgaata gatggctaaa
    21421 gaggctctgg gaaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg
    21481 cgcatcttat ccggcctacg gggagccagg ccgaataaat tcgtacgccg catccggcct
    21541 attttaaggc tgcgttttcc ccggcattcg cgggtcgtct ttatattcgg cggtggcaat
    21601 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    21661 cacggaacca aatgctgcgc cagagggtga tttcatcaac gatggcaggg tgtaggtcat
    21721 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgga tcagggcttt
    21781 tttgcgcgga cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc
    21841 gaagatggaa atcgccaggc caatcaaccg ccatgtcggt ttaagccact caatgctgtt
    21901 caggtgcagg gcgctaataa tcatttgctg cgcagaaccg gcaaccgagg tgatagaaag
    21961 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgca gatattttac
    22021 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgtgact gggcgcgaat
    22081 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccactg ccagaccgac
    22141 aagccctaca gtcgtccgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22201 tagcgtcgga tcgctaatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22261 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatgg ggatcatcga
    22321 caaaaatgag aaataggtga tagccgcccc aaactggtta cccagccgat cgttaaagcg
    22381 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggactttct cggcagtggt
    22441 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22501 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    22561 ctcttttgtc cttctgtcgt ggttattctt aaaaagtata gtcggtcagt ccaggtgctc
    22621 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    22681 ctgcggataa accagtgaaa gggacagcgg ctcggcacgg tagccaggca acacttcaat
    22741 aagccgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    22801 accggcaata ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    22861 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    22921 gtgcggtgta taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    22981 aaagcgcgtc agatagtgtg gactggcaca gttgaccatg gtcagtttgc cgagagaacg
    23041 ggcgataacg ccatcttccg gtaatgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23101 aagaatatct accggacggt catggctact cagttccagc tcaattcccg gatgcagata
    23161 gagaaattcc gacaagcgcg gcagtaacag gcttttcgcg attcctgacg ggatgtcgat
    23221 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23281 attactcaac acatcttttg ctcgttgata ataggtcatg ccttctggcg tgagtttgac
    23341 ccgtcgcgtg gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23401 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgct gcgcgggaaa aactttccag
    23461 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatctctt tgaccgattg
    23521 ttgttcacaa cgaaacagta gcgcgcatat cgcattgttt attgtttttt ctaacatcta
    23581 atgccgggtt tcacaatgtt gtcatgatga atgtggcgat ttctcagcag gaaaagggag
    23641 gaatagcttt atgagaaagg tgaattccct taaacagagc gtatttttca gcgattccct
    23701 ttaaagtgaa tagaagatgt attggctatt tgtgcttatt tttatctcat tgccgtttgt
    23761 tatttttgtt tatggtcgcg gaatttcatt tttgattgca tgcgtactaa ctaccgatat
    23821 aaggaaatcc tcaaaaatgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    23881 tgtaactatt tatatcggta actattttct acgcgttatc tctgtagaat ggccaccgcc
    23941 aaatggcgaa ttcttatttt ttctcgcgag taataccctt ccactactta agtcttttta
    24001 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24061 attcatggaa tgaaaatcag tttacagcat cgtattccag gagtgagtat tcagggggcc
    24121 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agtgatgctc
    24181 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcaattt
    24241 cctgaggtta aggtgttaat tacggcgacg gattgcaaca aacggtggtt acaggaagtt
    24301 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24361 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg aacgacgccg
    24421 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24481 ttagcggcgg gcgaatcaaa taaagagatc ggcagggcgc tgaatatcag taccggaacg
    24541 gtaaaagcgc atctggaatc actgtatcgc cgtctggaag tcaaaaatcg cacccaggcg
    24601 gcaatgatgt taaatatctc ctcctgaaga ggataatttt acagccagca ggaagttagc
    24661 ggctggctgt aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg taccacaccg
    24721 ttagtccagc caaacccatc ctgcaacgga tactcgccac cgccgccttc gcgaggaaca
    24781 ccatcggcaa tatggtattt ctcgatcagt ttgtgctgtt ccagatagaa ctgattcacc
    24841 gtcttcagcc agttgcgcgc gatttcatca cccagaaggt catcgccgta catcttaaat
    24901 ccctgaattg ccatccactg taacggtgcc cagccgttgg gtttatccca ctgttcaccg
    24961 gtttcgtact cgcttgccag aatcccgcca ggtgtcagta accggctgcg cacggcgttt
    25021 gccagacgat cggcctgttc atggttcgcc ataccgacat agagcggcac aatggcggca
    25081 gcggaaaaca gcgccagttg ctcgcgtcgc cagtcgtaat cgcggtagat gccgttttca
    25141 tcatcccaga ggtaacggtt taccgcatcg cgacgggcac tggccttctg gcggaacagt
    25201 gcttctgtct ctttctcgcc tttcagcgcc gagatgttgg cgatggcgct ctccagttta
    25261 aacaggaagg cattcagatc gatggggatg aactgggtgg tacgaatgct cgccagacga
    25321 ccagtatcac gcagccaacg ggaagagtaa tcccaaccgg aggccgcccc cgcacgtaaa
    25381 tcgcggtaca cctcgttagg cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc
    25441 caggattcgt cacgcggcgt gtcgcgatcg tcccagtaac ggttgagcag cgagccgtcc
    25501 ggcatccgca caacatggcg ataggcctga ttagggatca acgattctgc accgtccatc
    25561 cagaaggcat attccatttt caggtggtcg agatagcggc gcgcgccgcg tacaccatct
    25621 tcttcaaaca actccaccat cagcgcaaaa accggtggtt gcgagcggct caaataatag
    25681 gtgcggttgc cgttggggat gtggccgtag ttttcgatca tccaggcgaa gttatcagcc
    25741 atgcatttca gcaaatcttc ccgaccactt tccgccagcc ccagcatggt gaaataggaa
    25801 tcccaatagt aggtttcgct aaaacggccg cccgggacaa tatatgactg cggcagcgcc
    25861 agcagagaag accacggaat gtgatcctgt ggttcatgcg ttagcaccgg ccacagctgg
    25921 tcgatatgct ctttcaggga attttgcggg tccgatacat actcgctgga gtagacctcc
    25981 ggcagccaga agtggttttc aacaaacttg cgcaagtcaa aatcacgatg acggcgcact
    26041 ttacggtagc ggattaaaat atccagcggg tccattttgg gtgcgcagtc gggaaaggtt
    26101 ttactgtcgg ggaaaatttt cgccgactga acatgctcga acagttcgag atagcgatcg
    26161 gcaggcgtca gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc cggttctgcc
    26221 tcgatcatct catccagttt taattcatac gggtccagct catagcagag atcgacctcg
    26281 atcatcagtt cgtctggatt agggttttga attttctgat tgagcataag gatccgacct
    26341 ccgaaattcg tcgctaatta tcgggctttt ctttaagggt agtcattcgc tccgcctggt
    26401 gggcgacaaa gcgtgcggtg gatcacgttt attaacctgc caaaaaatat aacgcactaa
    26461 tatcaatgga atttataaaa atacggattt cgtgttgttt ttcattttca gaacattcaa
    26521 tcctgctctc gccgcaatca tgatatgcca ttcctgatct gggtaatagg cactggctat
    26581 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagca tatctccctg
    26641 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    26701 cacaataact cactaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    26761 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    26821 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    26881 tttaggcttt ctccgcgaaa aaggatgcga ttattgccat acgccttcgg cagaattacc
    26941 tgcctattat tatattcccg gcgcgaaaca gttgatggat tacgacataa agcttggata
    27001 taaatctttt aacctcgagg ccgtgcgtgc ggctctgctg gctaataaac ccgtttcgca
    27061 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccgc caacgcgtta
    27121 taccgcgctt cactgggcgg gtaagttgag tgatgaagag cgggcggaaa tactggcctg
    27181 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    27241 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    27301 ttttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    27361 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    27421 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    27481 ggatggtcgt gcggcaacat tgcaggatca ggctggtgga ccgccgttga acccgattga
    27541 aatggcgtcg aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    27601 agcgcagttc ctcgaagtct atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    27661 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    27721 agatgagaat gctctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    27781 atgtgcaact tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct
    27841 gaaaaaagac tttaactttg gtgaaattac ggcggcggat attggtcgta tgaatgttac
    27901 taaagaagag cgtgataaat tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac
    27961 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct
    28021 gcgctatcag gtaggcaaag agctgccgca ggaagatgtg gatgatatcg tagctttcct
    28081 gcacagtctg aacggggttt acacgccgta tatgcaggat aaacaataat taatttgatc
    28141 gcccgaacag gagtgtttgg gcgattttta ttacgataat aaagtctgtt tttaatatta
    28201 tcatattaaa tgtttatatt ataaaaagtc gtttttctgc ttaggatttt gttatttaaa
    28261 ttaagcctgt aataccttgc ttccattgcg gataaatcct acttttttat tgccttcaaa
    28321 taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact
    28381 cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct
    28441 gcacgaaatg cgcgatgatg ttgcatttca gattatcaat gatgaattat atcttgatgg
    28501 caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
    28561 attgatggat ttgtcgatca ataaaaactg gatcgacaaa gaagaatatc cgcaatccgc
    28621 tgcaatcgac ctgcgttgcg taaacatggt tgccgatctg tggcatgcgc ctgcaccgaa
    28681 aaatggtcag gccgttggca ccaacaccat tggttcttcc gaggcctgta tgctcggcgg
    28741 gatggcgatg aaatggcgtt ggcgcaagcg tatggaagcc gcaggcaaac caacggataa
    28801 accaaacctg gtgtgcggtc cggtacaaat ctgctggcat aaattcgccc gctactggga
    28861 tgtggagttg cgtgagatcc ctatgtgccc cggtcagttg tttatggacc cgaaaggcat
    28921 gattgaagcc tgcgacgaaa ataccatcgg cgtggtgccg actttcggcg tgacctacac
    28981 cggtaactat gagttccc
//
LOCUS       DAZFRE010000068        28816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFRE010000068 DAZFRE010000000
VERSION     DAZFRE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.068"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(85..1209)
                     /locus_tag="ACHKUI_003238"
     CDS             complement(85..1209)
                     /locus_tag="ACHKUI_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO6599386.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGASLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1342..2895
                     /locus_tag="ACHKUI_003239"
     CDS             1342..2895
                     /locus_tag="ACHKUI_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6599387.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3277..4167
                     /gene="metF"
                     /locus_tag="ACHKUI_003240"
     CDS             3277..4167
                     /gene="metF"
                     /locus_tag="ACHKUI_003240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6599388.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4496..6676
                     /gene="katG"
                     /locus_tag="ACHKUI_003241"
     CDS             4496..6676
                     /gene="katG"
                     /locus_tag="ACHKUI_003241"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6599389.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6770..7675
                     /gene="yijE"
                     /locus_tag="ACHKUI_003242"
     CDS             6770..7675
                     /gene="yijE"
                     /locus_tag="ACHKUI_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6599390.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7702..8319)
                     /locus_tag="ACHKUI_003243"
     CDS             complement(7702..8319)
                     /locus_tag="ACHKUI_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6599391.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8595..9698)
                     /gene="gldA"
                     /locus_tag="ACHKUI_003244"
     CDS             complement(8595..9698)
                     /gene="gldA"
                     /locus_tag="ACHKUI_003244"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6599392.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9709..10371)
                     /gene="fsa"
                     /locus_tag="ACHKUI_003245"
     CDS             complement(9709..10371)
                     /gene="fsa"
                     /locus_tag="ACHKUI_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6599393.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAFGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10383..12884)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_003246"
     CDS             complement(10383..12884)
                     /gene="ptsP"
                     /locus_tag="ACHKUI_003246"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6599394.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLVEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     AHHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13193..14272
                     /locus_tag="ACHKUI_003247"
     CDS             13193..14272
                     /locus_tag="ACHKUI_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6599395.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            14287..14607
                     /locus_tag="ACHKUI_003248"
     CDS             14287..14607
                     /locus_tag="ACHKUI_003248"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6599396.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14658..16955
                     /locus_tag="ACHKUI_003249"
     CDS             14658..16955
                     /locus_tag="ACHKUI_003249"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6599397.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16921..17799
                     /locus_tag="ACHKUI_003250"
     CDS             16921..17799
                     /locus_tag="ACHKUI_003250"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6599398.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17801..18142
                     /locus_tag="ACHKUI_003251"
     CDS             17801..18142
                     /locus_tag="ACHKUI_003251"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6599399.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18129..18980)
                     /locus_tag="ACHKUI_003252"
     CDS             complement(18129..18980)
                     /locus_tag="ACHKUI_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6599400.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19206..20939)
                     /locus_tag="ACHKUI_003253"
     CDS             complement(19206..20939)
                     /locus_tag="ACHKUI_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6599401.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALPYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21122..23773)
                     /gene="ppc"
                     /locus_tag="ACHKUI_003254"
     CDS             complement(21122..23773)
                     /gene="ppc"
                     /locus_tag="ACHKUI_003254"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6599402.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24075..25226)
                     /gene="argE"
                     /locus_tag="ACHKUI_003255"
     CDS             complement(24075..25226)
                     /gene="argE"
                     /locus_tag="ACHKUI_003255"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6599403.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25380..26384
                     /gene="argC"
                     /locus_tag="ACHKUI_003256"
     CDS             25380..26384
                     /gene="argC"
                     /locus_tag="ACHKUI_003256"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6599404.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26392..27168
                     /gene="argB"
                     /locus_tag="ACHKUI_003257"
     CDS             26392..27168
                     /gene="argB"
                     /locus_tag="ACHKUI_003257"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6599405.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRQLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27229..28602
                     /gene="argH"
                     /locus_tag="ACHKUI_003258"
     CDS             27229..28602
                     /gene="argH"
                     /locus_tag="ACHKUI_003258"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6599406.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
BASE COUNT         6680 a         7230 c         7903 g         7003 t
ORIGIN      
        1 atttcacggt catacattta tatatttaat taaatgaaag gtagtgatct ctcttaattt
       61 atggtcgttt gtcgagtgta ttgactaaca agatggagtg agcgattcat tgtattttaa
      121 cgccagtgag gtcagcatct gcgttacgcg acctgcggat aataacgcgc tttgggtaaa
      181 gtcgtgttgg tctgtcagcc atgtgataaa acaacgactg gttgccagag aaacatttgc
      241 gcccctttct aagctggctc ccgtgagtag gcagaccatc ttgcagttat tcccctgctc
      301 ccagcgctgc cactgatttt gccaccaacc atggtatgtc gttcccttaa aatgccaggg
      361 cgtatcagcg ttactgtcac tgatttcttc ttctacgaac caaagttcac cttgttgttg
      421 gcggaactga tctgcccagg ctgtaataat tgcgcctctt agaaaacatt cgtgtgaaat
      481 aatatgcgtt ttgacatctg gatagcggat tttcagtatt ctgaaatgcc cgtgtgccgt
      541 gataaccggg cgtagtatgg ctcccttacc gttgcgcttg agtacagcac tgccatactg
      601 aatttcatcg aactggctcc ggcgcaagaa ttgcgcttca cgcatatcta ccttctgcac
      661 gatatcgcga tggtgcatag ttggttctgt gttgcccttg taactgtacc gcagactgtc
      721 tcctgtctga tgttgcgtat aattcgtgga gagatgaaga atgttgccac gagtggcgtc
      781 aagactgact aaaacataca gtttttgtac tgcgctggcc ccttgaaagg gtatgaacag
      841 ttgaacggtt tcaatggcct ggggatgcgt aatttctttc tgatattttt taggcgtcca
      901 gacttttttg cagttacggc attgaatgcg ttgcgaaccc tgtggattgt gaccataaca
      961 aatcatgtct gtgccataac ataccgggca gaaatggcct ttctcaattg cataagtaga
     1021 caagtgaact gacaaccagt cacgaaattg ttgctcgtca aacaacggcg ggtaactgcc
     1081 acatgcccga cagtgcagtg ccggataacc cagtttatag tctggccaac tgtaatcttc
     1141 cgatgaggct aatcccaaat ttcggcaacc gaacgtttta caggcattaa tcgtaaacag
     1201 agtcgacata tatcaccaac gatttcacaa ggtcgttgat tttgtccggc aactatcccc
     1261 tgcggatgtg attactctca cataaataag tctttcagaa cgtaatgtag caatccttta
     1321 tctcactgaa agggattgtt tatgaaaatt aaaatacttg ctgcgggtat cgcattaaca
     1381 ctgccgttct gggcttgcgc taaagatgtc actattattt ataccaacga cctccatgcg
     1441 catgtagaac cttataaagt gccgtggatc gctgacggta aacgggatat tggcggctgg
     1501 gcaaatatca ctacgctggt taagcaagaa aaagcaaaaa ataaagcgac atggtttttt
     1561 gatgcgggcg actattttac cgggccatat atcagcagcc tgaccaaagg taaggctatt
     1621 atcgatatta tgaatactat gccgtttgat gccgtcacaa taggcaacca tgaatttgac
     1681 cacggctggg acaacacatt attacagttg agccaggcaa aattccctat tgtgcagggc
     1741 aatatttttt atcagaacag cagtaaatca ttctgggata agccatatac catcatcgaa
     1801 aaagacggtg tgaaaatagg cgttattggt ttgcacggag tatttgcctt taatgacact
     1861 gtctctgctg ctacgcgcgt gggtattgaa gcgcgcgatg aaattaaatg gctacaacgt
     1921 tatatcgatg aactcaaagg caaggttgat ttgaccgtcg ccctgatcca cgaaggtgtt
     1981 ccagcccgcc agtccagtat gggaggcaca gatgtgcgtc gcgcgttgga taaagatatt
     2041 cagacggcaa gtcaggtgaa agggctggat attttgatca ccggacatgc acatgtgggt
     2101 acgccggaac cgattaaagt cggcaatacg ctcatcctct ccactgacag cggcgggatt
     2161 gatgtgggca aactggttct cgactacaaa gagaagccgc ataattttac ggtgaaaaac
     2221 ttcgagctta aaaccattta cgctgacgag tggaagcccg atccgcaaac gaaacaggtg
     2281 atcgacggct ggaataaaaa gcttgatgaa gtggtgcaac aaacggtggc gcaatcgccg
     2341 gttgaattaa aacgtgccta tggtgaatcg gcttccctcg ggaacctggc ggctgacgct
     2401 ttactggcag cagcgggtaa aaatacccaa ctggcgttaa caaactctgg tggtatccgc
     2461 aatgagatcc ccgcaggggc gatcacgatg ggcggcgtta tcagtacgtt cccgttcccc
     2521 aacgaactgg tgacgatgga actcacgggt aaacaattac gcagtctgat ggaacacggc
     2581 gcaagtttaa gcaatggcgt tttacaggta tcgaaaggcc tggaaatgaa gtacgacagc
     2641 agcaagccgg ttggtcagcg ggtaatcaca ctgactctga atggcaaacc cattgaagat
     2701 gcgacggttt atcacattgc tactcagagt ttccttgctg atggtggaga tggttttacc
     2761 gcctttaccg aagggaaagc gcgtaacaca acgggcggtt attacgttta tcacgcggtg
     2821 gttgattact tcaaagcggg caacaccatt acggatgaac agctcaacgg tatgcgcgtg
     2881 aaggatatca aataaaagtg cgcactgaat tgactcaact gtttgttacc cgttgattgc
     2941 gcagatcctg tattcggcct acgcgagggc gcaggccgaa tcagatgcct taacatcacc
     3001 atccggtggc aggacgttgt ttttcgaatt agcccaggtg tcgctgtgtt gtcagaatat
     3061 gagtcaaaac gtgcgaggtg gattgcgcta tagagaaatc ttttatcgcc tctaattaag
     3121 atgagttttt ttcatcttcc ctgatttttc ctcaccatca ttggttattt ttcggttgac
     3181 gcccttcggc ttttccttca tctttacatc tggacgtcta aacggataga tgtgcacaac
     3241 acaacatata acgataagcg attgatgagg taaggtatga gcttttttca cgccagccag
     3301 cgggatgccc tgaatcagag tctggcagaa gtccaggggc agattaacgt ttcgttcgaa
     3361 tttttcccgc cgcgtaccag tgaaatggag cagaccctgt ggaactccat cgatcgcctt
     3421 agcagcctga aaccgaagtt tgtatcggtg acctatggcg caaactccgg cgagcgcgac
     3481 cgtacgcaca gcattattaa aggcattaaa gatcgcaccg gcctggaagc ggcaccgcat
     3541 cttacctgca ttgatgcgac gcctgccgag ctgcggatca ttgcacgcga ctactggaat
     3601 aacggtattc gccatatcgt ggcgctgcgt ggcgacctgc cgccgggaag tggtaagcca
     3661 gaaatgtatg cttctgacct ggtgacgctg ttaaaagaag tggcagattt cgatatctcc
     3721 gtggcggcgt atccggaagt acatccagaa gcgaaaagtg cccaggccga tttactcaac
     3781 ctgagacgaa aagtggatgc cggagccaac cgcgccatta ctcagttctt cttcgatgtc
     3841 gaaagctacc tgcgttttcg cgatcgctgc gtctcggcag gcattgatgt ggaaatcatt
     3901 ccgggaattt tgccggtttc gaactttaaa caggcgaaga aatttgccga catgaccaac
     3961 gtgcgtattc cggcgtggat ggcacaaatg ttcgacggcc tggatgacga tgctgaaacc
     4021 cgcaagctgg tgggcgcgaa tattgccatg gatatggtga agattttaag ccgtgaagga
     4081 gtgaaagatt tccacttcta tacgcttaac cgcgctgaaa tgagttacgc gatttgccat
     4141 acactgggtg ttcgacctgg tttataaata gtgtgccttt tgtgaaaatc acacagtgat
     4201 cacaaatttt aaacagagca caaaatgctg cctcgaaatg agggcgggaa aataaggtta
     4261 tcagccttgt tttctccctc attacttgaa ggatacaaat ctaaaaccct tttttataaa
     4321 gcatttatcc taaatcggac ataatcaaaa agtcttaatt acgatcaatt tgatctacat
     4381 ctctttaacc aacaatatgt aagatctcaa ctatcgcatc cgtggattaa ttcaattata
     4441 acttctctct aacgctgtgt atcgtaacgg taacactgta gaggggagca cattgatgag
     4501 cacgtcagac gatatccata acacgacagc caccggcaaa tgcccgttcc atcagggcgg
     4561 tcacgaccag agcgcaggtg ggggcacaac cacgcgcgac tggtggccaa atcaactccg
     4621 tgttgacctg ttaaaccaac attctaatcg ttctaacccg ctgggtgagg actttgacta
     4681 ccgcaaagaa ttcagcaaat tagattacta cggcctgaaa aaagatctga aagccctgct
     4741 gacagaatct caaccgtggt ggccagccga ctggggcagc tacgccggtc tgtttattcg
     4801 tatggcctgg cacggcgcgg gtacttaccg ttcaatcgac ggtcgcggcg gcgcgggtcg
     4861 tggtcagcaa cgttttgcac cgctgaactc ctggccggat aacgtaagcc tcgataaagc
     4921 gcgtcgcctg ttgtggccaa tcaaacagaa atatggccag aaaatctcct gggccgacct
     4981 gtttatcctc gcgggtaacg tggcgctgga aaactccggc ttccgtacct tcggttttgg
     5041 tgccggtcgt gaagacgtct gggaaccgga tctggatgtt aactggggtg atgaaaaagc
     5101 ctggctgact caccgtcatc cggaagcgct ggcgaaagca ccgctgggtg caaccgagat
     5161 gggcctgatt tacgttaacc cggaaggccc ggatcacagc ggcgaaccac tttcggcggc
     5221 agcagctatc cgcgcgacct tcggcaacat gggcatgaat gacgaagaaa ccgtggcgct
     5281 gattgcgggt ggtcatacgc tgggtaaaac ccacggtgct ggtccgacat caaacgtagg
     5341 tcctgatcca gaagctgcac cgattgaaga acaaggttta ggctgggcga gcacttacgg
     5401 cagcggcgtt ggcgcagatg ccattacctc tggtctggaa gtggtctgga cccagacgcc
     5461 gacccagtgg agcaactatt tcttcgagaa cttgttcaag tatgagtggg tacagacccg
     5521 cagcccggct ggcgcaatcc agtttgaagc ggtagacgca ccggaaatta tcccggatcc
     5581 gtttgatccg tcgaagaaac gtaaaccgac gatgctggtg accgacctga cgctgcgttt
     5641 tgatcctgag ttcgagaaga tctctcgtcg tttcctcaac gatccgcagg cgttcaacga
     5701 agcctttgct cgtgcctggt tcaaactgac gcacagggat atggggccga aatctcgtta
     5761 catcggaccg gaagtaccga aagaagatct gatctggcaa gatccgctgc cgcagccgat
     5821 ctacaacccg accgagcagg acattatcga tctgaaattc gcgattgcgg attctggtct
     5881 gtctgttagt gagctggtat cggtggcctg ggcgtctgct tctaccttcc gtggtggcga
     5941 caaacgtggt ggtgccaacg gtgcgcgtct ggcattaatg ccgcagcgtg actgggatgt
     6001 gaacgccgca gccgttcgtg ctctgccagt tctggagaaa attcagaaag agtctggcaa
     6061 agcttcgctg gcggatatca tcgtgctggc aggtgtggtt ggcgttgaga aagccgcaag
     6121 cgccgcaggt ttgagcattc atgtaccgtt tgcgccgggc cgcgttgatg cgcgtcagga
     6181 tcagactgac attgagatgt tcgaactgct ggagccaatt gctgacggtt tccgtaacta
     6241 tcgcgctcgt ctggacgttt ccaccaccga gtcactgttg attgataaag cacagcaact
     6301 gacgctgacc gcgccggaaa tgactgcgct ggtgggcggg atgcgcgtac tgggtgccaa
     6361 cttcgatggc agcaaaaacg gcgtcttcac tgaccgcgtt ggcgtattga gcaatgactt
     6421 cttcgtgaac ttgctggata tgcgttacga gtggaaagcg accgacgaat cgaaagaact
     6481 gttcgaaggc cgtgaccgcg aaactggcga agtgaaatac acagccagcc gtgcggatct
     6541 ggtctttggt tctaactctg tcctgcgtgc ggtggcggaa gtctacgcca gcagcgatgc
     6601 ccacgagaag tttgttaaag acttcgtggc agcgtgggtg aaagtgatga acctcgaccg
     6661 tttcgacctg ctgtaatctg accttcttca gcgactgcct ttcatgcagt cgctgaagtt
     6721 tctttaccgg cgtatagtgt ccacaggaaa actacacact ggatctctca tgtctgccgc
     6781 aggaaagagc aacccactgg caatcagtgg cctggttgtg ctcacactta tctggagtta
     6841 tagctggatt ttcatgaagc aagtcaccag ctacatcggt gccttcgact tcaccgcctt
     6901 acgctgcatt ttcggcgctc tcgttttatt catcgtcctt ttattacgtg gtcgcggaat
     6961 gcgcccgacg ccgtttaaat acaccttagc cattgccctg ttacaaacct gcgggatggt
     7021 tggcctggca cagtgggcgc tggtcagcgg cggagcgggg aaggtggcaa tcctgagcta
     7081 taccatgccg ttctgggtgg taattttcgc cgcgttgttt ctcggtgaac gcctgcgacg
     7141 tgggcaatat ttcgcgatac tgattgccgc tttcggctta tttttggtgt tgcagccgtg
     7201 gcaactcgat ttctcttcga tgaaaagtgc catgctggca atcctctccg gtgtgagttg
     7261 gggggcgagc gcgattgttg ctaaacgtct gtatgcccgc catccgcgcg tggatttatt
     7321 gtcgctaaca tcctggcaga tgctgtatgc ggcgctggtg atgagcgtgg tcgctttact
     7381 ggtgccgcaa cgtgaaattg actggcaacc caccgtgttc tgggcgctgg cctacagtgc
     7441 gattctggcg acagcgctgg cgtggagctt atggttgttt gtattgaaaa acttgcctgc
     7501 cagtattgcc agcttaagca cactggccgt tcccgtttgc ggcgtactct tttcctggtg
     7561 gctgctcggt gagaatccgg gggcagttga aggtagcggt attgtgctga ttgtgctggc
     7621 actggcgctg gtgagccgta agaaaaaaga agccgtcagt gtaaaaagga tctgaatttt
     7681 ttcttcatgt ggggagatct cttatttagc aaaataccga tagtgcccca ccatccgcca
     7741 gctaaacagt acatcttctt cctgcgcgcc tgcgccaatg ttatgtatca ccagcggcgt
     7801 accgtcgcgg gcgaagccat ctgacaccac cccaatatgt gccagcccgt tatctaatcg
     7861 ccaggagaca atatcgcccg cttgatagtc actggggttc ttgctggtgg ggcgtgtttt
     7921 atcgtggcgg gtaaaccagg tttccagatt aggcacccga cggtgatcga tgttgctgtc
     7981 cgggcgcttt aactgccatt tttgcgggta cgcagcaaaa ttcttcgcca tatcttcgtg
     8041 caccagtttc tgcaaatcga ccttctggct gcgcaatgca cggatcacca catcggaaca
     8101 tacgccgcgt tcttgcggga catcaccgcc aggataggta agctgcacat acgccggatc
     8161 gtaaaatagc gtgctgccga tttgctgtct ggcgccgtct gcgatggcaa gattggtgtt
     8221 agcctggatt tgtaccacgg ttggtggaac ggcgggagat tttaaggagt aactggtaaa
     8281 taccgtcaga agactgaata acgccagtga agctttcatc tgtttcgtcc gtgaaaataa
     8341 gagtcacaga ttcgaccttc cgggctaacg attgttctgt ggagaaaatg tccagaagcg
     8401 gataaaaaaa cacccgaaca aggtggaagt ttcagattac tggcacagcg tgccggatgc
     8461 ggcgtgaacg ccttatccgg cctacaaaag tacgtaaatt tactatattg cagagattgc
     8521 gtaggcctga taagcgtagc gcatcaggca attttgcgtt caaactcccg gacaagccgg
     8581 gagtttggag taggttattc ccactcttgc aggaaacgct gaccatactg gtcggctacc
     8641 agcagtgcgg cgtaaacctg atctggcgtc gcgccgccag gcatgttgtg gatggtttca
     8701 ccttctgcac atgccgcttc tgccacaatt cgcattttcg ccgggacatc ttctttaata
     8761 tccagttgag cgagagttat tggcaaacct accgcatggc taagcgcagc tacggtttcg
     8821 atttcctcca ccggcgcgtt ttccagaacc agctgcgtca gcgtaccgaa tgccactttt
     8881 tcaccatgat aatagtgatg cgcgtccggg atagcggtca ggccgttatg taccgcgtgt
     8941 gccgccgcca gcccgccgct ttcaaaacca acgccactca aataggtgtt cgcttcaatc
     9001 acgcgctcca gcgccggagt cactacatgc tgttcggcgg caagcatcgc tttttcgcct
     9061 tcttccagca gggtgttgta gcacagttca gccagtgcca gcgctgcctg agtgcacttg
     9121 ccacccgcca tggtggtcgc gccgctacgg gaacaggcac gcgcttcaaa ccaggttgcc
     9181 agcgcatcgc caatacccgc cgctaacaga cgcgcgggcg cgccagcgac gattttggtg
     9241 tcgacaatga ccatattcgg gttatttggc aacagcagat agcggtcaaa ctcaccctca
     9301 tcggtgtaga taacagacag tgcgctgcac ggtgcgtcgg tagaagcgat agtcggtgcg
     9361 atcgctaccg gaacacccat gaaatgtgcc agtgctttgg cagtatcgag ggtttttccg
     9421 ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg cagacggtcg
     9481 atctcatttt gcgaacattc accgccaaac ggcgcaattt ctactaccag tccagcatct
     9541 ttaaagcttt tctcgacagt ggattgagca aaacctaaaa caaatttgtc acccaccact
     9601 aaccagcgtt ccgccagtgg cttcaggtac tcgcccagac gattaattac atctgcgccc
     9661 tggatgtatt tacctggtga ttgaataatg cggtccataa ttactccttt agagatgagt
     9721 agtgccaaat gcggcattcc agtcgtgctc gaacttctct atagctgact ctaccgcagg
     9781 ggtgttaagc atttgttgcg ctacatctaa gggcagtgtg atggattcac atccagccag
     9841 caaacaatcc agcgcctgac gtggtgtttt aaagctggca gccagcacca tgctttctgg
     9901 cgcatgcatt tccagtaacg cttgcaactc ctgtacagta cgaatgccgt caccgccctg
     9961 ggcatctacg cggttaacgt atggagcaac gtatttggct ccagccagcg ccgccagtaa
    10021 tccttgcgcg gcgctgtaca ctgcggttcc cagcgtagta atgccttctt tcttcagcat
    10081 tttaattgct gccagacctt cagaggttac cggaattttc accacaatgc ccggaatagc
    10141 gtcgcgcagg cgtttcgctt ctttcaccat accctgcgcg tcgcggctca tggtctgagc
    10201 aaacagaatt ccctcatcac cgaacgcttt ttgcaggcgc ggcagcactt cccagatgga
    10261 ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatcg ggaatatgcg
    10321 tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca tgatgtttcc
    10381 ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta
    10441 accagcgcat tacggaattc ttcgtgcatg atgcgacgag cgaggcgcga gaaaatgcgc
    10501 atatgttgat cgcccgcggc gtgtttgttc agggttaaca taatgatgaa ttgcgcttca
    10561 tcatcgcccc agcgcactgg cgcttgcaga cgcgctacgc tgatggtgga ttgctcaatg
    10621 tgttccgatt tactgtgggg aatggcgaaa ctaaagccca gtccggtaga gaaaacggcc
    10681 tcgcgcgccc acaagtcggc ttccagttta cgcggatagc ggcagcggcc cgccagcagc
    10741 aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcgct ttccagtgtg
    10801 atgcactcgg cggtgaccag cggtgcgtcc tgttgggtca tgcggaattg cgccagcagg
    10861 tgttccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactg gcggcactca
    10921 cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg
    10981 agttcatcca gccctaaacc gaccagcaac ggcagcacgg aacctttcgc tcccagttca
    11041 ccgcacagac caatccattt gccctggcga tgcaccgcct gcacggcgta atcgagcgcc
    11101 cgcaagaagg ccggattcag gctgttgtag tgacgagtca ccttagcgtt atcgcgatcc
    11161 actgccagta gatattgcgt caggtcatta ctaccaatac taaagaaatc aatctcttcg
    11221 cagcattgat cgatgatgaa catcaccgac ggcacttcca gcatgatgcc gagctggatc
    11281 ttctcatcaa acggaatgtg ttcgttacgc agttgctgtt tggcttccgc cagtttttct
    11341 ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt caggctgccg
    11401 tgagcagagg cgcgcaggat cgaccgtagt tgtgtggtaa acaacgacgc gtattcttca
    11461 taaatacgca ccgcgcgata accgaggaac gggttagctt ctgccggaat gttcagataa
    11521 tcaacgggtt tatcaccgcc aatgtccata gtgcgcacaa taatgctgcg tccgttggca
    11581 gactccagcg cctgacaaaa aatgttgtac agctcgcttt cacccggtgc actggtgcga
    11641 tccatataga gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg
    11701 gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat accgtctgcg
    11761 gtacgcgctt gctgggtcag ccagacacgc tgttgctcac gcagagcgtc ctgtacgcgg
    11821 gcttcttgct gataataacg ggccacggat tcccctggct caaccacaat cgcccctgcg
    11881 ttgccgtcga tataaatcgt ttgatgttgc cacggagtaa gggcatcaat atccacacct
    11941 accagcgtcg gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt ggtgccgcca
    12001 cttttcagta atagaccttt gaggtgattt ttatccagtt cgaggaactg gctgggggtc
    12061 agttcatccg ccatacaaat ggcgggctgc gtcagtttgc ccggtgccgg gaagcgttgc
    12121 tcaccgtaga tttgctggag taactggaag cagacgtcgc gtacgtccag ggcgcgttct
    12181 tgcaggtagc tgctgctgga acgggaaaac tcttcgcaga agtggtgtgc actggcgaca
    12241 atcgcttcgg cacagcttaa tccggcgctg acacctgcca gtaaatgttc gcgcagggaa
    12301 gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc tggtagcacc gtcgctatcc
    12361 agcagacgaa actctatgtt tttcagtacc agcgtcagac cgttttccag tgcggattgt
    12421 tcggcgtcaa cgcctttggc tgcgggaaga ttacccagcg cattgagatc taaagaagag
    12481 atcggcgtca gaatgccgcc tgcgctaccg ctgcacacgg tgcgagcacg gataatttgc
    12541 ggattcagat tggtcagtga aatcggcagt ggttccagtt cgtcagattt tacttccacc
    12601 agcggtgcgt cgcagtgggg gaattcatcg cgcagccact ggcttaaacg ctggtgcgct
    12661 tcctgttcgt cggccccgga aatcagtaac tggcagttat cgcccaccag cgtatcggtg
    12721 ccaatcagcg caagggcgct tttggcgttg cccttgcggt cagtgcgcag gttatgccac
    12781 tcaatttgtg atgaaaaagt attacacagc gtttcaacgt ggcttgccgg acgcgcatgc
    12841 acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctt tctcccggta
    12901 cggttttctg tttacaggat aagagcgcac ggcaacggcc tgccatgtga caaatctgcc
    12961 aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt
    13021 tgcggcgtac ttccggatta tcgcaagtta tgaggcggat cgcatttttg tactgatatt
    13081 acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga ttgcctattg
    13141 tttcttccat caaaaatatg gacatgggct ttcgcaagcc cgggagcagc ttatgaatga
    13201 gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt cacacatgat
    13261 tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt atggcaaagg
    13321 cgcagtgccg gatgccgtag ccgatccgaa tctgaaaaaa ctgtttgata tcggcgttgc
    13381 aggtttgacg ctgatggtgc ctttcctcgc cgcttacatc ggttattcca ttgcagaacg
    13441 ttctgcgctg gctccatgcg ctatcggtgc ctgggttggt aacagctttg gcgcgggctt
    13501 ctttggtgcg ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat
    13561 tccggtgcat aaagttctgc gctcggtgat gccaatcttc atcattccaa tcgtcggcac
    13621 actgattacc gctggcgtca tgatgtgggg gctgggcgag ccggtggggg cgttgaccaa
    13681 cagcctgacc cagtggcttc aggggatgca gcagggcagc attgttatgc tggcggtgat
    13741 catgggtctg atgctggcgt tcgatatggg cggcccggtt aacaaagtgg cctatgcctt
    13801 catgctgatt tgcgttgctc agggtgttta taccgtggtg gctattgccg ctgttgggat
    13861 ttgtgttcca ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa atttctccgc
    13921 agaagagcgc gaaaccggca aggcggcact ggtgatgggc tgtgttggcg ttactgaagg
    13981 ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt ccttccatca tggtcggttc
    14041 agtttgtggt gcggtaactg cggcgctggt cggtgcgcag tgctatgcag gctggggtgg
    14101 tctgattgtg ctgccggtgg ttgaaggtaa gttgggttac atcgcggcag tggctgtcgg
    14161 ggcggttgtg acggcagtct gcgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc
    14221 ttcaactgaa gagaaggaag acgacctgga tctggatttt gaaattaatt aattgaggaa
    14281 cgagccatga cgaaaattat cgcagtaacc gcatgtcctt caggtgttgc ccatacttat
    14341 atggcggcag aggcgctgga aagcgccgcg aaagcgaaag gctgggaagt gaaagtagaa
    14401 acccaggggt cgattggtct ggaaaatgaa ctgactgcgg aagacgtggc aagcgccgat
    14461 atggtgattt tgaccaaaga tatcggcatc aaatttgaag agcgttttgc tggcaagact
    14521 atcgtgcgtg tcaacatcag cgacgcggta aaacgtgctg acgccattat gagcaaaatt
    14581 gaagctcacc tggcgcaaac cgcttaagtt cttatcccgc ccgcaagggc gggttcgctt
    14641 tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca ctgtttgcca
    14701 atacccgtga aatctcgctg gagcgggcgt tgctttatac cgccagccat cggcaaaccg
    14761 aaggggagcc ggtgataatg cgtcgggcga aagcaacagc gtatatcctt gaacacgttg
    14821 aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa ccacgcgccg
    14881 ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctggat caattcccga
    14941 cgcgtccgca ggaccgcttt gctatcagcg aagaagataa acgtatctac cgcgaagagt
    15001 tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag atgacggatg
    15061 aagtaaaagc cgcgaccagc acgcagattt tcagcatcaa ccagacggat aaaggccagg
    15121 ggcacattat tattgattac ccgcgcctgc tgaatcacgg gctgggggag ctggtggcac
    15181 agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggca gcgctgttac
    15241 tgctggaagc ctcgcagaaa catattttgc gttacgccga actggcggaa acgatggcgg
    15301 caagctgcac tgatggcccg cgtcgcgaag aactgctgac cattgcggag atctcccgcc
    15361 ataacgccga acataagccg cagacgttct ggcaggcgtg ccagttattc tggtacatga
    15421 acatcattct gcaatacgaa tccaacgcca gttcgctatc gttggggcgc ttcgaccagt
    15481 atatgttgcc gttctatcag acatcattaa cccagggcga agatccggca ttcctgaaag
    15541 aactgctcga atctttatgg gtgaaatgca acgacatcgt gctgttgcgc tccaccagta
    15601 gcgcgcgtta tttcgccggt ttccctaccg gctataccgc actgctcggc gggttaaccg
    15661 agaacggacg tagcgcggtg aacgtgcttt cgttcctttg ccttgacgct tatcaaagcg
    15721 tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac acccctttcc
    15781 tgatgaaaac cgcagaaacc attcgcctcg gcaccggtat tccgcaaatc tttaacgatg
    15841 aagtggtagt gccagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt
    15901 ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg catgacatcg
    15961 cgatgtttaa cctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa ggcaacgctg
    16021 cgctgactta tgaaggttta ctggaacaga tccgcgccaa gatcagccac tacatcaccc
    16081 tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggct cctgtaccgc
    16141 tactctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc gatggcggcg
    16201 cgcgttataa cttctccggc gtacagggga tcggtatcgc caacctgagc gattctctcc
    16261 atgcgttgaa agggatgatt tttgatcaac agcgtttaag ttttgacgaa ttgctgtcgg
    16321 tattaaaagc caacttcgcc acgccagaag gcgaaaaagt ccgcgctcgc ttaattaacc
    16381 gcttcgagaa atacggtaac gatatcgacg aggtggataa catcagcgcc gaactgttac
    16441 gccactactg caaagaagtg gaaaaatacc agaacccgcg cggcggctac ttcacgccgg
    16501 gatcgtatac cgtttctgcc cacgtcccgt tgggatcggt ggttggcgcg acgccagacg
    16561 gtcgttttgc cggggaacaa ctggcagacg gtggcttgtc acccatgctg ggtcaggatg
    16621 cacaagggcc aacggcggta ctgaaatcag tcagtaagct cgataatacg ctgctgtcta
    16681 acggtacgtt gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac
    16741 gcaaactggc cgacttctta cgggcgttta cccagcttaa gttgcagcat attcagttta
    16801 acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc
    16861 tggtggtgcg cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg
    16921 atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcaatgat
    16981 gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg
    17041 gtggtctttt ttaaaggctg tccgcatctt tgcccatggt gtgctaatcc ggagtcgatc
    17101 tccggcaaaa tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg
    17161 cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt
    17221 gacgctctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggtggc
    17281 gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgcgacccg ttttttacag
    17341 cgactgcggc tatggggtgt gtcatgcgcc atcgaaactg ccggagacgc gccagccagc
    17401 aagctgttac cgctggcgaa attgtgcgat gaagtgttgt tcgatttaaa aattatggat
    17461 gcggctcagg cgcgggatgt ggtgaagatg aacctgcaac gcgtgctgga gaatctgcgt
    17521 ttgctggtga gtgagggtgt caacgtgatc ccgcgtttac cgctgatccc tggtttcacg
    17581 ctcagccggg agaatatgca gcaggcgctg gacgtgctga tcccgctgaa tatcaagcag
    17641 atccatctgt taccgtttca tcagtacggc gagccgaaat accgcctgct ggggaaaaca
    17701 tggtcgatga aagaggttgc tgctccgtcg tcagccgatg tggcaacgat gcgtgaaatg
    17761 gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt
    17821 aaccgcctgc gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt
    17881 gtgccagtta gagaagtggg gcgttagcat tgaaactcag ggcgcgttgg gaacggagaa
    17941 tcgtttagcg gacgaggata tccgccgggc ggatgttgcg ctgttaatta cggatatcga
    18001 gcttgccggt gctgagcgat ttgaacattg tcgctacgtg caatgcagca tctacgcttt
    18061 cctgcgtgag ccgcagaggg tgatgagcgc agtgcgcaaa gtgctttctg cgccgcagca
    18121 aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccgacga
    18181 tattccgacg gcgagcgttc ggtattttta cgaaacagac ggcagaagta gttgctgtcg
    18241 acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt caataatgtc
    18301 ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt
    18361 tgaaacaggt gcgagaggta atttggcgaa atatagaacg cctgtgcgac agattcgcgg
    18421 gtaagtgcgg aggcatagcg ttcgtcgata taatcacgaa tagcttcgaa tagagcctgg
    18481 ctgcgtgagg cggtctgtat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg
    18541 gtgacgataa gtctggccgt ttgctgctcc tgcgactgca tttgcatttc attgagcgtt
    18601 tgtagcagaa aagaaccaat gcgcgggccg cgtcgggcga cgtgttgctt cgccagattt
    18661 tgatattgtt tgccatccca ttgcacaacg ctgaagccga gttgctgttt gccaaacagc
    18721 acgctaaagg tggtggcggg ggcttgccat tgtggaaaat tccagccacc agcagggacg
    18781 tacagcacat cgccgggaac taacgctgct ccagcgccgg tatcaataaa ctcaccttcc
    18841 agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgttg
    18901 ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga
    18961 tagctgacgt cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg
    19021 cattatgaaa aaaggccacc tgaaaagtgt ggcctgatgg gagtgagatg taaacattgc
    19081 ggcagtttca gtcgacttgt attgccggat gcggcgtaaa cgccttatcc ggcctaccgc
    19141 cgcgatctgt aggccggata agacgcgtca agcgtcgcat ccggcaaata acatctggct
    19201 gataattact gattacccac ctgatcgcca tacggcagag tgtcgtaatc gatcagtgcg
    19261 tttttcttat acgggttacc aatccagcgg gtagtttctt tgaattgcgg attcaccact
    19321 gaacgggttg gatcgtaacc gtcgtaagat aagcccgcca aatctgacca ggtgtggatc
    19381 agttccgcca ggctgtattt acgatcaaca tcctgtgaga aatcacgcgg atgagtcgct
    19441 tgccattttt ctgacgtcca cagcaggaac ggaatggtgt acatatgacg cgtcgggttg
    19501 tcttcattac gcccctgagt tttgtgcggc ggcgtgtcgt aaacctcttc accgtggtca
    19561 gagaaataaa ccaggaaacc gttcggatcg gtcgctttaa agtctttaat caggctggca
    19621 accacgtgat cgttgtacag gttagcattg tcataatcat tatatgactc cagctcttct
    19681 gcgtttaatc ccggtggaac atgctctgta ttaccgtcaa atttgccctg atcttccgga
    19741 taacggtact tgtacttgat atgcgtaccc agcagatgaa cgatgatcag tttcttcggc
    19801 gcagggtcct tcagcacttc ctggaacggc ttcagcacgt tggtgtcata ttcacgcgca
    19861 ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg tctggcgcga aaataccgtc
    19921 agcatggtat tgcgggcggt catcgtctgc tggttggtga tccagaaggt tttataaccc
    19981 gcctgtttca tcatgttcat cagcgacggc tgcgtcagat acagatccgg gttcttttca
    20041 ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt acggacgaga cgtaaccacg
    20101 ttattaaaca cggtcagatt cggatcggtt ttatgcagcg catccagctc cggcgtggtt
    20161 tcacgcggat aaccgtacag actcatgcgt ccacgctggg tcgattcgcc aattaccagc
    20221 actaaggtgc gcggttcgtt acccgattca tctttgaaat tagccagcgg cggcaaggca
    20281 ttattttcat tcagtagctt tgttagcgag tttagttgct gacgatactg ataatagccg
    20341 gtcaggaatt gccacggtgc ggcaggctcc atacgcgatg ccaggttatc caacgttttc
    20401 tcaaacggct tgttcttgat aaacgtattc atggcgatcg gatgcagaat caagccataa
    20461 agcagggcaa aagagacaac ataacgccac ggctttggaa tatagaccgg gcgcaggcgt
    20521 gtccacagca gaactgccac cgccgtatag ggcagcgcga taagcacaat tttcaggctg
    20581 aaatactggc ttaaatactc gctggcttcg ttggtgttgg tttcgaacat cacaaacaga
    20641 acgctctgcg agaactcctg accgtagatg acgtaatagc acagcgccgc cagagaggcc
    20701 gcccatagca ccacgccgat cactgcggca ataattttaa tccgcttcgg aaagaggaat
    20761 accgggatca accacagcga actgaataac agcgagtcgc gaatgccgtt agtgccacta
    20821 taaccactga tgtaaataat ggcctgtagc agagtagaga aaaaccaaaa gtagagcagt
    20881 gcccaaccca gggctttcca gctaaaaagg ggtttagcct ggacttctgt ggaatgcata
    20941 gtaagaacct gtcttgaaaa aaatatcgcc gaatgtaacg acaattcctt aaggatatct
    21001 gaagggatat ccagattttg aataaaatgc agacagaaat atattgaaaa cgagggtgtt
    21061 agaacagaag tatttcagaa aaccctcgcg caaaagcacg agggtttgca ggagaggaag
    21121 attagccggt attacgcata cctgccgcaa tcccggcaat agtgaccatt aacgcctgct
    21181 cgacgcgagg atccggttcc tggccttctt tttctgcctg gcgggagcgg tgcagcaact
    21241 cggcctgcaa tacgttcagc gggtcggtgt aaatattccg tagctgaata gactctgcaa
    21301 tccacggcag atcggccatc aggtgagaat cgttggcaat cgccagcacc actttgatgt
    21361 cttcttcttg cagattgcgt aactctttac ctaacggcca cagtgctttg tcgaccaggc
    21421 gttggtcata gtattctgcc agccacaggt ctgctttggc gaagaccatc tccagcatgc
    21481 cgagacgcgt cgagaagaat ggccaatcgc ggcacatggc ttccagctcg ctctgtttgc
    21541 catcttcgac cactttttgc agcgccgtac ctgcacccag ccaggcgggg agcatcaggc
    21601 ggttttgcgt ccaggcgaaa atccacggaa tagcgcgcag tgactcgacg ccgccggtcg
    21661 ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc caattcttgt tccggcgtag
    21721 cggagcggaa gtaaggcaca aaatctttgt tttcacgtac gtagccacgg tagagatcgc
    21781 aggagatgac tgacagttca tccataatgc gacgccagct ctctttcggc tcaggtggtg
    21841 gcagcaggtt ggcttccaga atcgccccag tgtacagcga caggctgctg acggtgattt
    21901 ctggcagacc atatttaaag cggatcatct cgccctgttc ggtcacgcgc aggccacctt
    21961 tcaggcttcc tggcggttgt gacagcagcg ccgcatgggc aggtgcgccg ccgcgaccaa
    22021 tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc cgctttttcg caggttttga
    22081 ttaatgcatc ctgtgcctgg tattgcgccc aggaagctgc catcacgccc gcatcttttg
    22141 ctgagtcgga atagccaatc atcaccatct gtttgccctg aatcaggccg cgataccagt
    22201 cgatattcag cagctgggtc atgacatcgt tggcgttgtt caggtcatcg agggtttcaa
    22261 acagcggagc aaccggcatc gcaaacccga tacccgcttc tttcagcagc aggtggacag
    22321 ccagcacgtc ggacggcgtt ttcgccatcg agatcacgta ggcggcaatg gagccttgcg
    22381 gcgcttcggc aatcacctgg caggtatcga gcacttcgcg cgtttcggcg cttggttgcc
    22441 agttgcgcgg tagaagcgga cgtttggagt tcagttcgcg gatcaggaac gcctgtttgt
    22501 cggcttctga ccagctctcg tagtcgccga taccgaggta gcgggtcagc tcgcccagcg
    22561 cttcggtatg gcgggtgctc tcctgacgga tatcaatacg gaccagcggt acgccgaaac
    22621 atttcacgcg gcgcagggtg tcgagcagat cgccgttggc gataataccc atgccacacg
    22681 cctgaagtga ctggtagcaa gcgtagagcg gttcccacag ttcttcgttt tgtgtcagca
    22741 ggccttctgg ttttggcagt tcttcgcctt tcaggcgcgc ttccagccat gcctgtgtcg
    22801 ccatcaggcg agaacgcagg tttttcatca gatagcgata cggttctgcg gcaccttctt
    22861 cgccaaccag cgccagcagt tcaggggtcg cttcaaccat cgacagttca gaaaccagca
    22921 cctgaatatc tttcaggaac agatcggtgg ctttccagcg actgagcagc aggacgtggc
    22981 gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc accgcccatc cacgaggtaa
    23041 aacggaccgg aacaaattcg acgggcagtt tgtagccgag attctcttcc agttgttcgt
    23101 tcagttcgcg caggtaattc ggtacgcctt gccacaggct gttttctact acggcaaagc
    23161 cccatttggc ttcatctacc gggcttggac gcagcttacg gatttcatcg gtatgccatg
    23221 actgggcgat caactggcgc aggcggcgca tcagctggtt atgttcgtag tcggcgatat
    23281 ctttgttatc gagctgtttt aagcaggcgt tcacttccac cattttgtgg atcagtgtac
    23341 gacgggtaat ttcggttggg tgagccgtga ggaccagttc cagcgacagc gattccactg
    23401 ctttttttat ggtgtcttcg ctcagttccg gctggttttt cagtttgcgc agggtgcggg
    23461 cgatcacttc cgggttgctg gcagcttcgc ctttcggcga aatgctgtgg tattgctcgg
    23521 cggtgttggc caggttcaga aactgactaa acgcacgcgc aacgggcagc agctcgtcgt
    23581 tcgacaaatt ttgtaaggtg gtgagcaact cctggcggtt agcatcattg ccagcgcgtg
    23641 aagatttgga caacttacgg atagtttcta cgcgttcaag aatgtgttct cccaacgcat
    23701 ccttgatggt ttctcccagc actttgccga gcatactgac attactacgc aatgcggaat
    23761 attgttcgtt catattaccc cagacacccc atcttatcgt ttgatagccc tgtatccttc
    23821 acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg tcgtctttta taaagccacg
    23881 taaaagcgat gacgtcaaat gctgcgaaat cgcttcagca aacgaataaa tagcaggaat
    23941 ttacgccatt aaattcacga cgctttaaat aagcgtaact tatgggaatg ttaaaaaatc
    24001 gcctcgagta acaccaaagg tgtaggtcgg ataagatgcg caagcatcgc atccgacatt
    24061 gtttggccta cgttttaatg ccagcaaaaa tggtgaatca cctgggtgat cagttcgcgg
    24121 gtgggcttga taaacctcgt ctccagatat tcatcaggct gatgagcctg attaattgag
    24181 ccaggcccca gaaccagcgt cgggcataac gtttgaataa acggcgcttc ggtacagtaa
    24241 ttcaccactt cggtctttgc tccgagcaat ttctcaacca cttcaaccag ttgatgattc
    24301 ggtgggcatt catagccagg gatcggcgga tgcagctcgt cgaccgtcag acgacccggc
    24361 cagcgttcgc tcactggagc caatgcatcg ttgagcaaac cgttaagttc attgagtgtc
    24421 atgcctggca gcggacgaat atccatatgc aactcacaac aagcgcaaat acggttagat
    24481 gcatcgccgc cgtggatatg cccgaggttg agcgtagggt atggcacggt aaacgcgtcg
    24541 tagtgataac gttctttcag gttatcgcgc aattgcaaaa tatgccctat ggcgtcgtgc
    24601 atcagttcga tagcgttaac tccgcgtgct ggatcgctgg agtggcccga ctggccctga
    24661 atacggatgg cgttagagat atgaccttta tgcgcgcgta ccggttgcag cgacgttggc
    24721 tcgccaatga tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata acgcgctccg
    24781 gccatactgg tttcttcatc ggcagtcgcc agaatgtaga gcggttttgc aagcttcgtg
    24841 acgtcgacat cgcgtagcgc atcaaggata aacgcaaaaa agcctttcat gtcggcggtg
    24901 cctaagccgt aaagcttgcc gtcatgctcc gtcaacgtaa atggatcgcg cgtccagcgt
    24961 ccgtcatcaa atggcaccgt atcggtatgc cccgccagca acaaaccgcc agccccctgt
    25021 ccgcaactgg ccagcatatt aaatttgttg cgagttcctg gcacaggctg tacttccaca
    25081 ttgaagccta aatccttaaa ccagtccgcc agcagagtga ttaaatctgc attgctttga
    25141 tcgagtgcct cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg gtaaatctcg
    25201 ataaatggcg gtaatttgtt tttcattgtt gacacacctc tggtcatgat agtatcaata
    25261 ttcatgcagt atttatgaat aaaaatacac taacgttgag cgtaataaaa cccaccagcc
    25321 gtaaggtgaa tgttttacgt ttaacctggc aaccagacat aagaaggtga atagccccga
    25381 tgttgaatac gctgattgtg ggtgccagcg gctacgctgg cgcagagcta gtgacctatg
    25441 taaatcgcca tccgcatatg aacataaccg ctttgactgt ttcagcgcaa agcaatgatg
    25501 cgggaaagtt aatctccgat ttgcatccgc agctaaaagg catcgttgat ctgccgttgc
    25561 agccgatgtc ggatatcagc gagtttagcc caggggtgga cgtggtgttt ctcgccaccg
    25621 cccatgaagt cagccacgat ttagcgccgc aatttcttga agcaggctgt gtggtgttcg
    25681 acctttccgg cgcgtttcgt gttaacgatg tcgccttcta tgaaaaatat tacggcttta
    25741 cccatcaata cccggaactg ctggaacagg cagcctacgg tctggcggag tggtgcggta
    25801 ataaattaaa agaagcgaac ttgattgccg tgccaggctg ttatccgacg gcagcacagc
    25861 tggcgctgaa accgttgatt gatgccgatc ttcttgacct caatcagtgg ccggtgatca
    25921 acgccaccag cggcgtgagc ggtgcaggac gtaaggcagc gatttcaaat agtttttgtg
    25981 aagttagcct gcaaccgtat ggcgtcttta cccatcgcca tcaaccagag atcgccacac
    26041 acctcggtgc tgacgttatc ttcaccccgc atctgggcaa tttcccgcgc ggcattctcg
    26101 aaaccattac ctgccgcctg aaaccgggtg tgagccaggt gcaagtggcg caagcgttac
    26161 agcaggcgta tgcccataaa ccactggtgc ggctgtatga caaaggcgtt ccggcgctga
    26221 aaaatgtcgt tggactgcca ttctgcgata tcggttttgc cgttcagggc gagcatctga
    26281 ttattgtggc gaccgaagac aacttactga aaggcgcggc ggcacaagcg gtgcagtgcg
    26341 ccaatattcg tttcggctat gcggaaacgc agtctcttat ttaagggtgc aatgatgaat
    26401 ccattaatta tcaaactggg tggcgtactg ctggatagtg aagaggcgct ggaacgtctg
    26461 tttagcgcac tggtgaatta tcgcgagtca catcagcgtc agctggtgat tgtgcacggc
    26521 ggcggttgcg tggtggatga gctgatgaaa gggctgaatc tgccggtgaa aaagaaaaat
    26581 ggcctgcggg tgacgcctgc ggatcagata gacattatca ccggagcact ggcgggaacg
    26641 gcaaataaaa ccctgctggc atgggcgaag aagcatcaga ttgcggccgt aggtttgttt
    26701 ctcggtgacg gcgacagcgt caaagtgacc cagcttgatg aagagttagg ccatgttgga
    26761 ctggcgcagc caggttcgcc taagcttatc aacagcttgc tggagaacgg ttatctgccg
    26821 gtggtcagct ccattggcgt aacaaacgaa gggcaactga tgaacgtcaa tgccgaccag
    26881 gcggcgacgg cgctggcggc aacgctgggc gcggatctga ttttgctctc tgacgtcagc
    26941 ggcattctcg acggcaaagg gcaacgtatt gccgaaatga ccgccgcgaa agcagaacaa
    27001 ctgattgagc agggcattat tactgacggc atgattgtga aagtgaacgc ggcgctggat
    27061 gctgcgcgca cgttgggccg tccggtagat atcgcctcct ggcgtcatgc ggaacagctt
    27121 ccggcactgt ttaacggtat gccgatgggt acgcggattt tagcttaagt tttgttggcc
    27181 ggaggcgcag ctttccggca ttgaatttca aaataaggaa acagagttat ggcactttgg
    27241 ggcgggcgtt ttacccaggc agcagatcaa cggttcaaac aattcaacga ctcactgcgc
    27301 tttgattacc gtctggcgga gcaggatatt gttggctctg tggcctggtc caaagccctg
    27361 gtaacggtcg gcgtgttaac cgcagaagag caggcgcaac tggaagaggc gctgaacgtg
    27421 ctgctggaag atgttcgcgc caggccacaa caaatccttg aaagcgacgc cgaagatatc
    27481 catagctggg tggaaggcaa actgatcgac aaagtgggtc agttaggcaa aaaactgcat
    27541 accgggcgta gccgtaatga tcaggtagca actgacctga aactgtggtg caaagatacc
    27601 gttagcgagt tgctgacggc taaccggcag ttacaatccg ctctggtcga gaccgcgcag
    27661 aacaatcagg acgcggtgat gccgggttac acccacctgc aacgcgccca gccggtgacg
    27721 ttcgcgcact ggtgcctggc ctacgttgag atgctggcgc gtgatgaaag ccgtttgcag
    27781 gatgcgctta agcgtctgga tgtcagcccg ctaggctgtg gcgcactggc aggaacggcc
    27841 tatgaaatcg accgtgaaca gttagcaggc tggctgggct ttgcttcggc gacccgtaac
    27901 agtctcgaca gcgtttctga ccgtgaccat gtgttggaac tgctttctgc tgccgctatc
    27961 ggcatggtgc atctgtcgcg ttttgctgaa gatctgattt tctttaacac cggcgaagcg
    28021 gggtttgtgg agctttctga ccgtgtgact tccggttcat cattaatgcc gcagaagaaa
    28081 aacccggatg cgctggagct gattcgcggt aaatgcggtc gggtgcaggg ggcgttaacc
    28141 ggcatgatga tgacgctgaa aggtttgccg ctggcttaca acaaagatat gcaggaagac
    28201 aaagaaggtc tgttcgacgc gctcgatacc tggctggact gcctgcatat ggcggcgctg
    28261 gtgctggacg gcattcaggt gaaacgtccg cgttgccagg aagcggcgca gcagggttac
    28321 gccaacgcta ctgaactggc ggattatctg gtggcgaaag gcgtaccgtt ccgcgaggcg
    28381 caccatattg tgggtgaagc ggtggtggaa gccattcgtc agggcaaagc gctggaagat
    28441 ctgccgctca gtgagttgca gaaattcagc caggtgattg gcgaagatgt ctatccgatt
    28501 ctgtcgctgc aatcgtgcct cgacaagcgt gcagcaaaag gcggtgtctc accgcaacag
    28561 gtggcgcagg cgattgcttt tgcgcaggcg cggttggggt aatttgttta tattgctccc
    28621 ctgaaaatgg ggagcttacc acgcctacaa agtgtgacac ttcgtaggcg gataaggcgt
    28681 ttacgccgca tcaggcagaa atttgtcagc attctggtga ccttaaattt aacgcccaac
    28741 cagaaccatt atttgtcaca ttccttttta ttcttgcgtc ttctttacct tcactctccc
    28801 cataaaaatt atattt
//
LOCUS       DAZFRE010000069        28581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFRE010000069 DAZFRE010000000
VERSION     DAZFRE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.069"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(18..193)
                     /locus_tag="ACHKUI_003259"
                     /pseudo
     CDS             complement(18..193)
                     /locus_tag="ACHKUI_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(404..1249)
                     /gene="araC"
                     /locus_tag="ACHKUI_003260"
     CDS             complement(404..1249)
                     /gene="araC"
                     /locus_tag="ACHKUI_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6599407.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHSEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            1588..3288
                     /gene="araB"
                     /locus_tag="ACHKUI_003261"
     CDS             1588..3288
                     /gene="araB"
                     /locus_tag="ACHKUI_003261"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6599408.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQHAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKDQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEKHYLPTSAPAQAAQAVPTL
                     "
     gene            3299..4801
                     /gene="araA"
                     /locus_tag="ACHKUI_003262"
     CDS             3299..4801
                     /gene="araA"
                     /locus_tag="ACHKUI_003262"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6599409.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            5086..5781
                     /gene="araD"
                     /locus_tag="ACHKUI_003263"
     CDS             5086..5781
                     /gene="araD"
                     /locus_tag="ACHKUI_003263"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6599410.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            5856..8207
                     /gene="polB"
                     /locus_tag="ACHKUI_003264"
     CDS             5856..8207
                     /gene="polB"
                     /locus_tag="ACHKUI_003264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6599411.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATGSGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFCLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGLKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            8372..11278
                     /gene="rapA"
                     /locus_tag="ACHKUI_003265"
     CDS             8372..11278
                     /gene="rapA"
                     /locus_tag="ACHKUI_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6599412.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            11290..11949
                     /gene="rluA"
                     /locus_tag="ACHKUI_003266"
     CDS             11290..11949
                     /gene="rluA"
                     /locus_tag="ACHKUI_003266"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6599413.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(12067..12882)
                     /gene="djlA"
                     /locus_tag="ACHKUI_003267"
     CDS             complement(12067..12882)
                     /gene="djlA"
                     /locus_tag="ACHKUI_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6599414.1"
                     /translation="MQYWGKIIGVTVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            13137..15491
                     /gene="lptD"
                     /locus_tag="ACHKUI_003268"
     CDS             13137..15491
                     /gene="lptD"
                     /locus_tag="ACHKUI_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6599415.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVAASNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15544..16830
                     /gene="surA"
                     /locus_tag="ACHKUI_003269"
     CDS             15544..16830
                     /gene="surA"
                     /locus_tag="ACHKUI_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6599416.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            16830..17819
                     /gene="pdxA"
                     /locus_tag="ACHKUI_003270"
     CDS             16830..17819
                     /gene="pdxA"
                     /locus_tag="ACHKUI_003270"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6599417.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            17816..18637
                     /gene="rsmA"
                     /locus_tag="ACHKUI_003271"
     CDS             17816..18637
                     /gene="rsmA"
                     /locus_tag="ACHKUI_003271"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6599418.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            18640..19017
                     /gene="apaG"
                     /locus_tag="ACHKUI_003272"
     CDS             18640..19017
                     /gene="apaG"
                     /locus_tag="ACHKUI_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6599419.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            19024..19872
                     /gene="apaH"
                     /locus_tag="ACHKUI_003273"
     CDS             19024..19872
                     /gene="apaH"
                     /locus_tag="ACHKUI_003273"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6599420.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(19869..20183)
                     /locus_tag="ACHKUI_003274"
     CDS             complement(19869..20183)
                     /locus_tag="ACHKUI_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO6599421.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(20186..20419)
                     /locus_tag="ACHKUI_003275"
     CDS             complement(20186..20419)
                     /locus_tag="ACHKUI_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6599422.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENTEAIAALNQLADETGCFSDEYRSF"
     gene            complement(20505..20984)
                     /gene="folA"
                     /locus_tag="ACHKUI_003276"
     CDS             complement(20505..20984)
                     /gene="folA"
                     /locus_tag="ACHKUI_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6599423.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(21176..23038)
                     /gene="kefC"
                     /locus_tag="ACHKUI_003277"
     CDS             complement(21176..23038)
                     /gene="kefC"
                     /locus_tag="ACHKUI_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6599424.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASNASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(23031..23561)
                     /gene="kefF"
                     /locus_tag="ACHKUI_003278"
     CDS             complement(23031..23561)
                     /gene="kefF"
                     /locus_tag="ACHKUI_003278"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6599425.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGTHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(23669..25000)
                     /gene="yaaU"
                     /locus_tag="ACHKUI_003279"
     CDS             complement(23669..25000)
                     /gene="yaaU"
                     /locus_tag="ACHKUI_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599426.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(25059..25346)
                     /gene="fixX"
                     /locus_tag="ACHKUI_003280"
     CDS             complement(25059..25346)
                     /gene="fixX"
                     /locus_tag="ACHKUI_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6599427.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(25343..26629)
                     /gene="fixC"
                     /locus_tag="ACHKUI_003281"
     CDS             complement(25343..26629)
                     /gene="fixC"
                     /locus_tag="ACHKUI_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6599428.1"
                     /translation="MFEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(26680..27621)
                     /gene="fixB"
                     /locus_tag="ACHKUI_003282"
     CDS             complement(26680..27621)
                     /gene="fixB"
                     /locus_tag="ACHKUI_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6599429.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(27636..28406)
                     /gene="fixA"
                     /locus_tag="ACHKUI_003283"
     CDS             complement(27636..28406)
                     /gene="fixA"
                     /locus_tag="ACHKUI_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6599430.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAVEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         6872 a         7888 c         7448 g         6373 t
ORIGIN      
        1 agctgcgcct gatgccgcta cacttttgcc agaccatgtt ctcctctgag ggagtaactg
       61 gcgtttttca tagaatgccg tacttttttc aacaactgcc acatcgaacg gcatttatgg
      121 tggcgtgaag tgcctgccat aaccgctcaa tgtggtgttc ctacgatgaa caaaccgctt
      181 gatatattct cctgatgcaa ccggacaata tgttcatgat gggtatcccg atacataagc
      241 cttcgtttta tttttacaaa aagaaatcgt tgtcgatatt ttatcaagaa aaaaataatt
      301 caggtaaaac gattcaacta aaaataacta aaattaatcc cccgtgaaat atataaataa
      361 cgtcagatga agaaagcaag ggggttcccc cccttctttc ttattattca caacctgccc
      421 taaactcgct cggactcgcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat
      481 catcaaaacc gacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      541 gcttcgcctg actgatgcgc tggtcctcgc gccagcttaa tacgctaatc cctaactgct
      601 ggcggaacaa atgcgacaga cgcgacggcg acaggcagac atgctgtgcg acgctggcga
      661 tatcaaaatt actgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      721 tatccatcgg cggatgaagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      781 caagcagatt tatcgccagc aattccgaat agcgcccttc cccttgtccg gcattaatga
      841 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaaccgg
      901 tattggcaaa tatcgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      961 aaacccactg gtgataccat tcgtgagcct ccgaatgacg accgtagtga tgaatctctc
     1021 caggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
     1081 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     1141 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     1201 cgttaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     1261 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     1321 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
     1381 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1441 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1501 ccataagatt agcggatcca gcctgacgct ttttttcgca actctctact gtttctccat
     1561 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
     1621 gattctgtgc gagctttggc ggtggactgc gccaccggtg aagagatcgc caccagcgta
     1681 gagtggtatc cccgttggca gaacgggcaa ttttgtgatg ccccgaataa ccagttccgt
     1741 catcatccgc gtgactacat tgagtcaatg gaagcggcgc tgaaaactgt gcttgcagag
     1801 cttagcgtcg aacagcacgc agctgtggtc gggattggcg ttgacacaac cggctcgacg
     1861 cccgcaccga ttgacgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
     1921 ccgaacgcga tgttcgtatt gtggaaagac cacaccgcgg ttgaagaagc ggaagagata
     1981 acccgcttat gtcacacgcc gggcaacgtt gactactccc gctatattgg cggtatttat
     2041 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
     2101 caatctgccg catcgtggat tgagctgtgc gactgggtac cagctctgct ttccggtacc
     2161 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg gacataagtc gctatggcat
     2221 gaaagctggg gcggcctgcc gccagccagt ttctttgacg aactggaccc gatcctcaat
     2281 cgccatttgc cttccccgct gttcactgat acctggactg ccgatattcc ggtgggcacg
     2341 ttatgtccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
     2401 gcgtttgact gccatatggg cgcagttggt gcaggcgcac agcctaacgc actggtaaaa
     2461 gttatcggta cttcgacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
     2521 gcagtgaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
     2581 gaagcaggcc aatcggcgtt tggcgatatc tacgcctggt tcggtcgcgt actcggctgg
     2641 ccgctggaac agcttgccgc ccagcatccg gaactgaaag atcaaatcaa cgccagccag
     2701 aaacaactgc ttccggcgct gaccgaagca tgggctaaaa acccgtctct ggatcacctg
     2761 ccggtagtac tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
     2821 ggggtgatta ccgatctgaa cctcgctacc gacgctccgc tactgttcgg cggtttaatt
     2881 gccgccaccg cctttggcgc acgcgcaatt atggagtgct tcaccgatca ggggatcgcc
     2941 gtcaataacg tgatggcact gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
     3001 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
     3061 ggtgcggcga ttttcgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
     3121 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgt gcagcgaaca ggcacaacgc
     3181 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaaa acactatctt
     3241 ccaacttccg ccccggcaca ggctgcccag gccgttccga ctctataagg acacgacaat
     3301 gacgattttt gataattatg aagtgtggtt tgtaattggc agccaacatc tgtatggccc
     3361 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtc gttaatgcgc tgaatacgga
     3421 agcgaaactg ccctgcaaac tggtgctgaa accgctgggc accacgccgg atgaaatcac
     3481 cgctatttgc cgtgacgcga attacgacga tcgttgcgct ggcctggtag tgtggctgca
     3541 cactttctcc ccggccaaaa tgtggatcaa cggcctgact atgctcaaca aaccgttgct
     3601 gcaattccac acccagttta acgcggcgct gccgtgggac agtatcgata tggactttat
     3661 gaacctgaac cagactgcac atggcggtcg tgagttcggc ttcatcggcg cgcgtatgcg
     3721 tcagcaacat gccgtggtta ccggtcactg gcaggataaa caagcacatg agcgtattgg
     3781 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgttt
     3841 tggcgataac atgcgtgaag tggcggtcac cgatggtgat aaagttgccg cacagatcaa
     3901 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
     3961 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
     4021 tgccacacaa atccacggcg aaaaacgcca gaacgtgctg gaagcggcgc gtattgagct
     4081 ggggatgaag cgcttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
     4141 tttgcacggt ctgaaacagc ttcctggtct ggccgtacag cgtctgatgc agcagggtta
     4201 cggctttgcg ggcgaaggcg actggaaaac tgccgccctg cttcgcatca tgaaggtgat
     4261 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
     4321 aggcaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga tcgccgcaga
     4381 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
     4441 cctgattttc aatacccaaa ccggcccagc gattgtcgcc agcttgattg atctcggcga
     4501 tcgttaccgt ctactggtta actgcatcga cacggtgaaa acaccgcact ccctgccgaa
     4561 actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
     4621 gtggatcctc gctggtggcg cgcaccatac cgttttcagc catgcgctga acctcaacga
     4681 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
     4741 cctgccagcg tttaaagacg cgctgcgctg gaacgaagtg tattacggat ttcgtcgcta
     4801 agtagccgaa tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat caggcctaca
     4861 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaccgc ctgatgcggc
     4921 gctggcgcgt cttatcaggc ctacgcgttc cggatttgta ggccggataa gcaaagcgca
     4981 tccggcattc accgcctgat gcgacgccga cgcgtcttat caggcctaca cgctgcgatt
     5041 ttgtaggccg gataagcaaa gcgcatccgg cacgaaggag tcaacatgtt agaagatctc
     5101 aaacgccagg tattagaagc caacctggcg ctgccaaaac acaacctggt cacgctcacc
     5161 tggggcaatg tcagcgccgt tgatcgcggg cgcggcgtcc tggtgatcaa accttccggc
     5221 gtcgactaca gcatcatgac cgccgacgat atggtcgtgg ttagcatcga aaccggtgaa
     5281 gtggttgaag gaacgaaaaa gccctcctcc gacacgccaa ctcaccggct gctctatcag
     5341 gcattcccgt ccattggcgg cattgtgcac acacactcgc gccacgccac catctgggcg
     5401 caggcgggtc agtcgattcc agcaaccggt accacccacg ccgactattt ctacggcacc
     5461 attccctgca cccgcaaaat gaccgacgca gaaatcaacg gtgaatatga gtgggaaacc
     5521 ggtaacgtca tcgtagaaac cttcgaaaaa cagggtatcg atgcggcgca aatgcccggc
     5581 gtcctggtcc attctcacgg cccatttgca tggggcaaaa atgccgaaga tgcagtgcat
     5641 aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg
     5701 cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggtgcg
     5761 aaggcatatt acgggcagta atgactgtat aaaaccacag ccaatcaaac gaaaccaggc
     5821 tataatcaag cctggttttt tattggattt tcagcgtggc gcaggcaggt tttatcttaa
     5881 cccgacactg gcgggacacc ccacaaggta cagaagtctc cttctggctg gcgacgggca
     5941 gcgggccgtt gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt cccgccgatc
     6001 aggttccccg cgcccagcat attttgcagg gtgagcaagg cttttgcctg acgccgctgg
     6061 cgttaaagga ttttcatcgc cagccggtgt atggccttta ctgtcgcgcc catcgccagt
     6121 tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc
     6181 gcccgccaga acgctatctg atggagcggt ttatcacctc gccagtgtgg gtcgagggtg
     6241 atatgcgcaa cggcgctatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc
     6301 cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg tactgcatcg
     6361 gcctggaagg ttgcgggcag cgtatcgttt atatgctggg gccagagaat ggcgacgcct
     6421 ccgcgctcga ttttgaactg gaatacgtcg ccagccgccc gctgctactg gaaaaactta
     6481 acgcctggtt tgcgactcat gatcctgatg tgatcatcgg ttggaacgtg gtgcagttcg
     6541 atctgcgaat gctgcaaaaa catgccgagc gttaccgtat tccgctgcgt ctggggcgtg
     6601 ataacagcga gctggagtgg cgcgagcacg gcttgaaaaa cggcgtcttt tttgctcagg
     6661 ccaaagggcg actgattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct
     6721 cttcattctc gctggaaacc gtcgctcagg agttattagg cgaaggaaaa tctatcgata
     6781 acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataag cctgcgctgg
     6841 caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa actgaaatca
     6901 tgccgttttt gctcgaacgg gcgacggtga acggcctgcc ggtagatcga cacggcggtt
     6961 cggtggcagc gtttggtcat ctctattttc cccgtatgca tcgcgctggt tatgtcgcgc
     7021 ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg gattcgcgac
     7081 ctgggcttta tgattcggtg ctggtgctgg actacaaaag cctgtacccg tcgatcatcc
     7141 gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct gatccagagc
     7201 acagtaccga aggttttctc gatgcctggt tctcacggga gaaacattgc ctgccggaga
     7261 ttgtgactaa catctggcac ggacgcgatg aagccaaacg tcagggtaat aagccgcttt
     7321 cgcaggcgct gaagatcatt atgaatgcct tttatggtgt gctcggcacc accgcctgcc
     7381 gtttcttcga tccacgcctg gcatcgtcga tcaccatgcg tggccatcag atcatgcggc
     7441 aaaccaaagc gttgattgaa gcgcagggct acgacgtgat ctacggcgat accgactcaa
     7501 cgtttgtctg gctgaaaggt gcacattcag aagaagaagc ggcgaaaatc ggtcgtgcac
     7561 tggtgcagca cgttaacgcc tggtgggcag aaacgctgca aaaacaacgg ctgaccagcg
     7621 cactggaact ggagtatgaa acccatttct gccgttttct gatgccaacc attcgcggag
     7681 ccgataccgg tagtaaaaag cgttatgccg ggctgattca ggagggcgat aaacagcgga
     7741 tggtgtttaa agggctggaa accgtgcgca ccgactggac gccgctggcc cagcagtttc
     7801 agcaggagct ttacctgcgt atcttccgta acgagccata tcaggaatac gtccgcgaaa
     7861 ccatcgacaa actgatggcg ggtgaactgg atgcgcggct ggtttatcgt aaacgccttc
     7921 gccgtccgct gagcgagtat cagcgtaatg tgccgcctca tgtacgcgcc gctcgccttg
     7981 ccgatgaaga aaaccataag cgcggtcgcc ccttgcaata tcagaaccgc ggcaccatta
     8041 agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt
     8101 acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg
     8161 aggataattt tgctacactt atgaccgggc aacttgggct attttgagca aaaaaaagag
     8221 ttcgccagat accattttga tgcgtgacga atgctttgcc atccagtacc atagcgccct
     8281 ttccattcct ggacctgaat aacaccacta cctcataagc acggtagcgg gtggttattg
     8341 cctgcaatta aagatataga gccgaacaca tatgcctttt acacttggtc aacgctggat
     8401 cagcgataca gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt
     8461 cactttactt ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt
     8521 gacccgcgtg atgttcaacc ctggtgatac cattaccagc catgacggct ggcagatgca
     8581 agtcgaagaa gtaaaagaag aaaatggctt gctgacctat atcggtactc gccttgatac
     8641 tgaagagtcc ggcgtggccc tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa
     8701 accgcaggac cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta
     8761 tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca
     8821 gcgtaccagc ctgatcccgc accagctcaa catcgctcat gatgttggtc gccgccacgc
     8881 gccgcgcgtc ctgttggctg acgaagtggg tttagggaaa accattgaag ccgggatgat
     8941 cctgcatcag caactgctct ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac
     9001 cttacagcat cagtggctgg tagaaatgct gcgccgtttc aacctgcgct ttgctctgtt
     9061 tgatgatgag cgttatgccg aagctcagca cgatgcttac aacccgttcg ataccgagca
     9121 gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa cagcgtctgg aacatctctg
     9181 tgaagccgag tgggatctac tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga
     9241 tgcgccgagc cgtgaatatc aggccattga acaactggca gagcacgtgc cgggcgttct
     9301 gctgctgacc gcaaccccgg aacagctggg gatggaaagc cacttcgccc gtctgcgtct
     9361 gctggacccg aaccgtttcc acgactttgc gcaattcgtt gaagagcaga aaaattatcg
     9421 tccggttgcg gacgccgttg ccatgctgct ggcaggtaac aaactgagca atgacgaact
     9481 gaacatgctc ggtgagatga tcggcgagca ggatatcgag ccgctgttgc aagcagcaaa
     9541 cagcgacagc gaagatgccc agagcgcccg tcaggaactg gtttcgatgc tgatggatcg
     9601 ccacggtacc agccgcgtgc tgttccgtaa cacccgtaac ggtgtgaaag gcttcccgaa
     9661 acgcgagctg cacactatta agctgccact gccgacgcag tatcagaccg ctattaaagt
     9721 ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgc gctcgcgata tgctctatcc
     9781 ggagcgtatt tatcaggaat ttgaaggcga taacgccacc tggtggaact tcgatccgcg
     9841 cgttgagtgg ctgatgggct acctgaccag ccatcgctct cagaaagtgc tggtgatctg
     9901 cgccaaagct gccactgcgc tgcaactgga gcaagtactg cgcgaacgtg aaggtattcg
     9961 cgccgcggtg ttccacgaag gcatgtcgat aatcgaacgt gaccgcgctg ccgcctggtt
    10021 tgctgaagaa gacaccggtg cgcaggtact gctgtgctcg gaaatcggtt ctgaaggacg
    10081 taacttccag ttcgccagcc acatggtgat gtttgacctg ccattcaacc cggatctgct
    10141 ggagcagcgt attgggcgtc tggatcgtat cggtcaggcg cacgatattc agatccatgt
    10201 gccttatctg gaaaaaacgg ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga
    10261 tgcattcgag cacacctgcc cgaccggacg cactatctac gatagcgtgt acaacgattt
    10321 gattaactat ctggcctcgc cggatgagac cgaaggcttt gacgatctga tcaaaaactg
    10381 ccgcgagcaa catgaagcgc tgaaagcaca gctggaacaa ggtcgtgacc gcctgctgga
    10441 aatccattcc aacggtggcg aaaaagccca ggcactggca gaaagcattg aagagcagga
    10501 tgacgatacc aacctgatcg ccttcgccat gaacctgttc gatattatcg gtatcaatca
    10561 ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga
    10621 cttccctggc ctgtcggaag atggcatcac catcaccttc gaccgtgaag tggcgctggc
    10681 gcgtgaagat gcacagttta ttacctggga gcatccgctg atccgcaacg gtctggatct
    10741 gatcctttct ggcgataccg gtagcagcac gatttcactg ttgaaaaaca aagcgttgcc
    10801 ggtaggtacg ctgttggtgg aactgattta tgtggttgaa gcccaggctc cgaagcagtt
    10861 gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg ctgctggata aaaacggcaa
    10921 caacctggcg gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg cggttaaccg
    10981 tcacaccggc agcaagctgg ttaacgcggt gcagcaggat gttcacgcta tccttcagct
    11041 gggggaagcg cagatcgaga aatctgcccg tgcactgatt gatgcagcgc gtaacgaagc
    11101 cgacgaaaaa ctgtctgccg agctgtctcg tctggaagcg ctgcgtgcag ttaacccgaa
    11161 cattcgtgac gacgaactga ccgccattga gagcaaccgt cagcaggtaa tggaaagcct
    11221 ggatcaggca ggctggcgtc tggatgccct gcgtttgatc gttgtaacgc atcagtaacg
    11281 gagccgaaaa tggggatgga aaactacaat ccaccgcagg aaccctggtt ggttatcctg
    11341 tatcaggatg accatattat ggtggtcaac aagccgagcg gtttgttgtc tgtgccgggt
    11401 cgtctggaag agcacaaaga cagcgtgatg acgcgcattc agcgtgatta tccgcaggca
    11461 gaatcggtgc atcgtctgga tatggctacc agcggcgtga ttgtagtggc gctgaccaaa
    11521 gccgcggagc gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa acagtatgtg
    11581 gcccgcgtct gggggcatcc atcccctgcg gaaggtctgg tggatctgcc gctgatttgc
    11641 gactggccaa accgcccgaa acagaaagtc tgttacgaaa caggtaaacc tgcgcagacg
    11701 gagtatgaag tggtggaata tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt
    11761 accgggcgtt cgcatcagct gcgtgtgcat atgctggcgc tgggtcatcc gattctcggc
    11821 gatcgttttt atgcatcacc agaagcgaga gcaatggcac cgcgtttgtt gctacatgca
    11881 gagatgctga cgattaccca tccggcgtat ggtaatagta tgacatttaa agcaccagcg
    11941 gatttttaat agtcacgctg gcgaagttcg gtaggccgga gaaggcgttc acgccgcatc
    12001 cggcaatttt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag gcctacacgt
    12061 tgaaacttat ttaaaccctt tctgctgctt tatcagctca taggcctgct gaatttcctg
    12121 cgctttctgc ttcgccatct ccatcatctc tggcggcaaa cctttcgcca ccaacttatc
    12181 gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg catcatccgt
    12241 cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct gcgcttgctg
    12301 ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac cgccctgcat
    12361 catgcgcaaa aactggtcaa actgagcgcg ggagatccct aattcttcag caatgacata
    12421 cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct gaatctggat
    12481 ctccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa actggcgcat
    12541 cttttcgcgc agcgggtaat tgtctgattt tcccacccgg aacgcatttt gcgccgcagt
    12601 acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat gaatatcagc
    12661 ttccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag tggtggcaaa
    12721 aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc tacgggcttt
    12781 atcaaacata tggccaatta acaggcccaa tactacgccc caaaagccgc cgcccattat
    12841 taaggccacg gtcacgccaa tgatttttcc ccaatactgc atatattccc caaatcgaca
    12901 cacggatatc agggctatct cccacaatat aaaggcgctt ttacccattt ctggctgcgg
    12961 tctattgtga gacatcgcct ataatttgca ttatcatacc tgtcattcaa tgccgtgcct
    13021 aacaccacag acgctattcg gacaggatta acactagcgt agagatgacg agtacgttag
    13081 tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat caacgtatga
    13141 aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat agtcaacagg
    13201 gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat gaccgtcctc
    13261 tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac gcgaaagggg
    13321 actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt aacagccgtc
    13381 tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg gagccggtac
    13441 gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc ctcaaagggc
    13501 cgaaaggctg ggcgaatctg aacaccaaag ataccaacgt ctgggagggt gattaccaga
    13561 tggtgggtcg ccagggccgc ggtaaagcgg acctgatgaa acaacgtggc gaaaaccgct
    13621 ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac acctggagcg
    13681 tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgcggagatc tggaacgccc
    13741 gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg ccggtgggtg
    13801 acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc aactactttg
    13861 agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc atcacgccgc
    13921 attatatgca tcgtcgtggc aatatcatgt gggagaacga attccgctac ctctcccagg
    13981 cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat aaagatgaac
    14041 acccgaacga tgacagttca cgtcgttggt tattctactg gaaccactcc ggggtcatgg
    14101 atcaggtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc tacttcaatg
    14161 atttcgataa caagtacggt tccagtaccg acggctacgc aacgcaaaaa ttcagcgttg
    14221 gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag gtcttcagcg
    14281 agcagaacac cagtagctac tcggcagaac cgcagttaga cgttaactac tatcaaaacg
    14341 atgtcggtcc tttcgatacg cgaatttacg gccaggcggt gcactttgtt aacaccagag
    14401 atgacatgcc agaggcgact cgtgttcacc tggaaccgac catcaatctg ccgctctcta
    14461 ataactgggg cagcatcaat actgaagcga agttgctggc aacccattat cagcaaacca
    14521 atcttgactg gtataactcc agaaacacga ccaggctggc agagtccgct aaccgtgtaa
    14581 tgccgcagtt caaggtagac ggtagaatgg tcttcgagcg tgatatggaa atgctggcgc
    14641 cgggctatac ccagacactg gaaccacgcg cgcagtatct gtatgtgcca tatcgcgatc
    14701 agagcaaaat ctataactac gactcgtcgc tgctgcaatc tgactactcc ggcctgttcc
    14761 gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaggtg acgaccggtg
    14821 tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc gttggtcaaa
    14881 tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag aatgacgaca
    14941 aaacgggctc actggtatgg gcaggcgata cttactggcg catctctgat cgttggggat
    15001 tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcggccagt aactccagca
    15061 ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccgttac gccagcccgg
    15121 aatatattca ggcaacgctg cctaagtact attccactgc tgagcaatat aagaatggta
    15181 tttcgcaggt aggtgccgtc gccagctggc caattgccga tcgttggtcc attgttgggg
    15241 cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt gtgcaataca
    15301 gctcctgctg ctatgcgatt cgcgtcggtt acgagcggaa gctgaacggt tgggataacg
    15361 ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt cgcggcctga
    15421 gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg ccgtatcaaa
    15481 acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg aaatggaaaa
    15541 agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc
    15601 gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt cgtgctggaa
    15661 agcgacgttg atggattaat gcagtcggta aaactgaacg ctgctcaggc aaggcagcaa
    15721 cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat ggatcaaatc
    15781 atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct ggatcaggcg
    15841 attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag ccgtctggct
    15901 tacgatggtc tgaactacaa cacctatcgt aaccagatcc gcaaagagat gattatctct
    15961 gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccgcagga agtcgaatcc
    16021 ctggcgcagc aagtgggtaa ccaaaacgac gccagcactg agctgaacct gagccacatc
    16081 ctgatcccac tgccggaaaa cccgacctct gatcaggtga acgaagcgga aagccaggcg
    16141 cgcgcgattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc gattgctcat
    16201 tctgccgacc agcaggcgct gaacggcgga cagatgggct ggggccgtat tcaggagttg
    16261 ccggggatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt tggcccgatt
    16321 cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga aagcaaaaat
    16381 atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc gatcatgact
    16441 gacgaacagg cccgcgtgaa actggaacag attgctgccg atatcaagag tggtaaaacg
    16501 acttttgctg ccgcagcgaa agagttctct caggatccag gctctgctaa ccagggcggt
    16561 gatctcggct gggctacacc agatattttc gatccggcct tccgtgacgc cctgacccgc
    16621 ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca tttaatcgaa
    16681 ctgctggata cccgtaatgt cgataaaacc gacgcggcac agaaagatcg tgcataccgc
    16741 atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga acaacgtgcc
    16801 agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt gtgatcactc
    16861 ccggcgagcc cgccgggatt ggcccggact taattgtcca gcttgcacag cgtgagtggc
    16921 cagtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca gcgatgctcg
    16981 gtttgccgct caccctccgt ccttattccc ccaactcccc tgcacaaccg caaactgcgg
    17041 gcacattaac gctacttcct gtcgcgctac gtgaatctgt cactgcgggg cagttagcgg
    17101 ttgaaaatgg gcattatgtg gtggaaacgc tggcgcgagc gtgtgatggc tgtctgaacg
    17161 gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac gacgcaggca
    17221 ttccgtttac cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa aaagtggtga
    17281 tgatgctggc aaccgaagaa cttcgcgtgg cgctggcaac gacgcattta ccgctgcgcg
    17341 atatcgcaga tgctatcacc cctgcgcttt tgcacgaagt gattgctatt ttgcatcacg
    17401 atttgcgcac caaatttggt attgccgaac cgcgcattct ggtctgcggg ctgaatccac
    17461 acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt ccggtgctcg
    17521 acaagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat accctgtttc
    17581 agccgaaata tcttgataac gccgacgcgg tgctggcgat gtaccacgat cagggtcttc
    17641 ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc ctgcccttta
    17701 ttcgcacatc agtggaccac ggtaccgcgc ttgaactggc gggacgtggt aaagccgatg
    17761 tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac acccaatgaa
    17821 taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact ttctcaacga
    17881 tcagttcgtg atcgacagca ttgtttctgc cattaacccg cagaagggcc aggcgatggt
    17941 cgaaatcggt cccggtctgg cggcattgac cgaaccggtc ggcgaacgtc tggaccagct
    18001 gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc cattcttagg
    18061 cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg aactggccgc
    18121 gaaaatgggc cagccgctgc gtgttttcgg caacctgcct tataacatct ccacgccgtt
    18181 gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta tgttgcaaaa
    18241 agaagtggtg aatcgtctgg ttgcaggacc gaacagcaag gcgtatggtc gattaagcgt
    18301 catggcgcaa tactattgca acgtgatccc ggtgctggaa gtaccgccgt cagcctttac
    18361 accaccaccc aaagtggatt ccgccgtcgt gcgcctggtt cctcatgcaa cgatgcctca
    18421 cccggttaaa gatgttcgcg tgttgagccg catcaccacc gaagccttta accagcgtcg
    18481 taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa cgggaatggg
    18541 gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc agatggcgaa
    18601 ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc gccccgagtg
    18661 tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa tgaacgttac
    18721 gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca gttgttgggg
    18781 cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg cgaaggagtg
    18841 gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag cggtgcaatc
    18901 attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga aaatggcgtc
    18961 cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat tcattaaaag
    19021 aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact gatcgcattg
    19081 ctgcataaag tagaatttac ccctgggaaa gataccctct ggctgacggg cgatctggtc
    19141 gcgcgcggcc cgggttcgct ggatgttctg cgctatgtga aatccttagg cgacagcgta
    19201 cgtctggtgc tgggtaatca cgatctgcat ctgctggcgg tatttgccgg gatcagccgc
    19261 aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga cgagctgctt
    19321 aactggctgc gtcgccagcc tttgctgcaa atcgacgaag agaaaaagtt ggtgatggcc
    19381 cacgccggga tcacgccgca gtgggatctg cagaccgcca aagagtgcgc gcgcgatgta
    19441 gaagcggtgc tgtcgagtga ctcctatccc ttctttcttg atgccatgta cggcgatatg
    19501 ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat caccaacgcc
    19561 tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag caaagaatcg
    19621 ccggaagagg cccctgcccc actgaaaccg tggtttgcga ttcctggccc cgtcgctgaa
    19681 gagtacaaca tcgcctttgg tcactgggca tcgctggaag gcaaaggtac gccggaaggt
    19741 atttacgcgc tggataccgg ctgctgctgg ggtggtacat taacctgcct gcgctgggaa
    19801 gataaacagt attttgtcca gccgtcgaac cggcataagg atttgagtga gggagaggcg
    19861 gtagcgtctt aaatcccgtc gagcataaaa tctaatgctc ctttaatcaa cgttcggtgc
    19921 gcgctggcat cgcaaaattt cgttcccagc gcgttaactg gaacagttgc agtctcatgc
    19981 gtcatgagca catattcttt gccgtctacc agtaaaagga ttgggttaag ccgttctggt
    20041 gggcgaatac ggctaaatcg ttcaataggt gttaatggaa taacgatacg gcgattaatc
    20101 tcaccaataa tgtcgctggt aacatcaata acaaagggaa acgcggcgtt cctgccgcga
    20161 ctgcggtata ccgtaaattg catcgctaga agctccggta ctcatcagag aaacatccgg
    20221 tttcatcagc caattgattt aacgcagcga tagcttctgt gttctcttca cgccaacgtg
    20281 ttgctgcatg ttttttaagt tcagcattga gcgcaaccgt aagggtggcg ctcatattta
    20341 agcctgcatc acgagcgcga ttgactaact cacggtcgac ggtgacggtc acactttgtg
    20401 tggtacgttt tgcagtcatg atctcgtgct cctgtacaat ttatacgtct agtatacacg
    20461 gatatctgca cgtatcagtg atgccggaat tctaatatac gaaattaccg ccgctccaga
    20521 atctcaaagc aatagctatg agagttctgc gcatcagcgt cgtggaattc gctgaatacc
    20581 gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac ttccgcgtcg
    20641 atatgcgtca gatacagttt ttgcgctttc ggcaggaact gctcataaac gcgaccgccg
    20701 ccaatcacca tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgacttc
    20761 acccacgtta cgcgatcgtc cgtacccggt tgactgctga gaataatatt tttgcgtcct
    20821 ggcaacggac gaccgatgga ttcccaggta tggcggccca taatcacggg tttatttaaa
    20881 gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg
    20941 ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa
    21001 aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc gaggatgatg
    21061 ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac tcgccgcatt
    21121 acaatgaaac aaaaacaaac agttagctgt aaagcgtgat ttacgtcact ctttattagg
    21181 atgagggctt cgtttccggt tcatccgcca tattgccagt atgtttccct tcttcggttc
    21241 cctgccagcc atgtcgttga attaatgaca gatgttcacg gtcttcggta atgatctcac
    21301 ttaacatcgc gctggtgcgt ttatagaccg ccgcacgggc tttggtgtcg ttctcaacca
    21361 ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggcg cgttctcgcg
    21421 cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc agcgcacctt
    21481 cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg tagtggtcga
    21541 catcgcgggc gcgggcaata atctgcaaat gtgggaaatg ttctttcacc atctccgtca
    21601 gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct ttcgccgctc
    21661 cggcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc ataccgaatt
    21721 tacgcagcgt ttcgatatgg tccgggtcgt gatcgagcac caccattttc accccgctgg
    21781 agagcagtaa acgtccggtt atctgcccaa aacgcccgaa tccggcaatg attacccgcg
    21841 gctgttcttc gtcgatctca tcggcttcac gcgcctcttc agtagaagat tgctcaaggc
    21901 gattgaggat caccagcaga atcggcgttg ccgccatcga cagtgccacc gccagggtca
    21961 gcgatttcgc ccactctggc tccagcacat tcgccatctg cgccgcacca aatactacaa
    22021 aggcaaactc actgccctgt cctaacagca ccgcaaacca gcgacgctgt ttatttggca
    22081 cctgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg aagccgagca
    22141 gcaaaataac aatgcgcaac ggattttcaa gcagcgtgcc aaagtctatc gacatgccaa
    22201 cgccgatgaa aaacagcccc agcagcaaac ctttaaatgg ttcgatatcg ctttccagcg
    22261 catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc atcgccatcg
    22321 acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac gccacggcac
    22381 taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc gtgacatagc
    22441 gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag agagcaaatg
    22501 cgcccatcgt cgtcgaggca ttgctcgccg ccagtagcgg gatcatcgcc accagcggga
    22561 tcgccgcgat atcctggaac agcagcaccg caaaggcact gcggcccatt tgcgtcacca
    22621 tcagattgcg ttcattcatc gcctgcatgg caattgccgt agaggagagc gccagcgtca
    22681 tgccgatcaa ttccgctacc tgccagcgca gcccaagtaa catgcagaac agccccagca
    22741 ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca cgcagcttcc
    22801 acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc acgccaatct
    22861 cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac gggccaataa
    22921 tgcagcctgc tatcaggtag ccaagtaccg atcccagacc gaggcgtact gcaatgggta
    22981 caatcagcgc cgccgaaccg agataaatca gcgcctgaat caaggtatga ctatccatga
    23041 tgtgcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc ttcgagggtt
    23101 tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt cagcccgcag
    23161 tagattgccg tcgcctgtag cggctgcgac agcacatcaa accccggatg cgtgccaatc
    23221 tcaaaatggc tttccccacc gccggtcgtc accgcccaca gtaaatgttt gccatgcagc
    23281 gccgtgccgc cgtgaccgta agcccagccg tgcgagaaga ctttatcgat ccaaagtttg
    23341 aggagcggag gaatgctgta ccactgcatc gggtgctgcc agacgatcag atcggcacga
    23401 gacagcgcct cctgttcggc ggcaatgtcg atattgaagt caggatagag ttgataaaga
    23461 gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt attcgcatgg
    23521 gaatgatgcg gatatggatg cgcataaatt ataagaatca taggtagccc gcgtgattac
    23581 gcctttgttt tcccacaaag tgtagtcata tattcacgca gcttatagtc aataatattg
    23641 attcagaaaa gagagaaatc tgaacaaatt accccattct ctgcccgcgg atcgtcatat
    23701 tgctggtctg cgccagtgac atccctcgag tctccggggc aaacgctacg gaaatcaaca
    23761 agccaaatag cgagataccc gcccccatta gcatcgtgtt actgataccg taattattga
    23821 taaagatcgg cagtgcccag gtcgatacaa tagtgccaat acggcttaag gacataatca
    23881 cgcccacggc agaggcgcgg atatccgtcg ggaagagttc attaggataa agccactgca
    23941 aattacccgg cccaccggag aaaaaggcat acaccgcaaa cgccattacc accagccaga
    24001 tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg gcaaagctgc
    24061 caatcaacaa tggacgccgt ccggcagtgt ttagccacag catcggcgga atacagccga
    24121 gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca acccccaatc
    24181 ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg atcacctggc
    24241 aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta aacagatcgc
    24301 gaaaacgggt ttgctgcggc tgctcttcct cgaaagccac cggttcgcca aacagtttta
    24361 tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa caaccagcgg ggagattcag
    24421 gcagttcgaa tcgaccaatc aaaatcaaca aacagggaat cgctgcgcta cccagcatcc
    24481 agcgccagcc gccttccaca tcataaagcc aatagccgac caaatcggcg caggtcgcgc
    24541 cgacatacca catcgcggca ataaagctga tggaaaatgc ccgctgacgg gtactggaga
    24601 actcggtgat cattgaggtg gcgatgggat aatccgcacc gataacaatg ccgataagta
    24661 cccgcatcac caacagttcg acgggggatg agacaaacat cgtcgccacc gatatcacgc
    24721 cgatggcgat gatatcaatg aggaacattt tgcgccgtcc gactttatcg gagatatagc
    24781 caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccagc aagccaatcc
    24841 agtcagcgtc cagtttcagc gccggagtca gttgctccag cgccacgcca atcattacca
    24901 gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg cggtgaatag
    24961 aggagaattt aagatcatca aagtttctgg acggttgcat gggttattcc tgtttttttg
    25021 caatgagagt cagtacggca ttacgtatca aaccaacatc agccgtaacg gaactccaca
    25081 ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc cagaatgcga
    25141 caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc atcctgcttc
    25201 ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt atcagcatcg
    25261 gccttcacaa cgatgtgcgg atgctcttca tcgacattga atttattgac gcccagtttg
    25321 acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca tccttgatca
    25381 gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc agttccggcg
    25441 cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc gggtagccgc
    25501 taaacatgcg tgggttatca agaaacgccg gtagtttctg gtacatgcgc atatcgcgca
    25561 gcgggccact ctcaagatgc tgacgatatt ccgcgagttt ttgcttactg aaatcgtcgc
    25621 ttttcatcgc tgaaagcaca gtttttgctg ctgcttcccc ggcggcaatc gccagatcca
    25681 taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcacca gcaatcaata
    25741 cgccgtcacc gaccagctcc ggcagcatgt tgatgcccgc ttccggcact acgtgagcgg
    25801 aatattccac cagcttgccg cccgcgatca gcggtgcaac ggccggatgt tgtttgaaat
    25861 cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaaa ccacaaacca
    25921 gccccagcga cagggtattt tcattggtat aaaggaagcc acctcccatc aggccatcgg
    25981 tgggtgatcc cgcaaacagg caagccgcgc cctgattacc ctgcaactga aaacggtctt
    26041 caataaccga cttcggtaac tcgatcagtt ccttcacgcc aactgccaca tccgtcggtt
    26101 taacgcgttt tgccatcccc agcttttcgg caaggatgga attcacccca tcagcaagga
    26161 tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact ttgccatcgc
    26221 gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct tcttcggcct
    26281 gctccatcag ccaggcatca aatttactgc gtaaaacgga gtaagaacgc tgggatggcg
    26341 aggtttcgtc gccattgcag tagtccatag tcatcgctga cttttccgtc ataaacgcaa
    26401 gtttttcatg ggtgatcagg cgttctacgg gggcggagtc agcgaaacca ggaataatgt
    26461 gttccagact gtgggcatag agacgcccgc cggtgacgtt cttggcacct gcggaattgc
    26521 ctcgctcgat aactaacact tgcgcacctt cgcgggcgag caccagtgcg gcaaccgaac
    26581 cggcaagccc tgcaccgacg atgatggcgt caaagatatc ttcgaacata agagctccct
    26641 gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta aagctgcggt
    26701 cagtgccgga aggatcttca cggcgtcgcc aacaatgccg taatccgcgt actggaagat
    26761 cggcgcattt ttatctttgt tgatggcgaa aatggtttgc gacgcattag cgccaaccat
    26821 gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca gcatcaggtt
    26881 ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca ccggacgaga
    26941 acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa tgttctcttt
    27001 gctgccaata ccgcgaccaa cgctgaccac caggcgggct ttgtcgagat cgacgctgtt
    27061 gctctgacgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct gccactccac
    27121 ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc acatcgaacg tgccgctgct
    27181 gatggtcaga accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca gaccgccgta
    27241 aaccatgtgt ttcactgtcg ctttaccgtc ctgcacgcta acagcgctgg catcgttaga
    27301 caccgccgcg ttaaggcggt aacccagttt cgccgccagt aatttgccgc gacgggtgtt
    27361 tggcaacagc accagaccgt ctgcgccgtg ctggcgaata gtgtcagcca tgacaccggc
    27421 gtaatcttcg atcatccgct cgtccggttt gccgcttaat ttccagacat gattagcgcc
    27481 gagctggatt gcctgtgcgc cgtcggcatc attgaggacg aacgtgttga tttgattagc
    27541 taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc tgaataccca
    27601 gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg aagattttca
    27661 gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg acgctcgcgc
    27721 tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa accgatatcc
    27781 gccgccgacc atacctggac tggctttttc gccgcgccga gaatggcttt catcgaagga
    27841 atttgtgggg agttgatatc agtggaaaca gcaacaaccg caggcagcgg aatgcttaag
    27901 gtttctgttt catcttccag ttcgcgctca acggtgaggg tatctgccgt cagggagata
    27961 attttgctga cgccgttaac tgccggaata ttaaggattt cgcctactag caaaccaacc
    28021 tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa gcctgctttc
    28081 tgagcggcag cagccagtac gctcgccgtt tgttgcggca gtgcctgctc gaactggtca
    28141 tcaatcacca caatcagttc atccggaccg cgcgatagca catctttacg ccctttggcg
    28201 ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctac tgcctgttgc
    28261 tttagctggc aagccgcttc aatagcgttg agatcgtatt ggcttatttt ggcatcggct
    28321 ttgctgaagt ctaacgaacc atcagcatta ttgaccgcaa tatcctgttc atcaggcacg
    28381 cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga aacgtcgttc
    28441 atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa actttaatca
    28501 ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta agctcctcac
    28561 caatattgaa aacatggcgt a
//
LOCUS       DAZFRE010000070        28578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFRE010000070 DAZFRE010000000
VERSION     DAZFRE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.070"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            134..9754
                     /gene="clbB"
                     /locus_tag="ACHKUI_003284"
     CDS             134..9754
                     /gene="clbB"
                     /locus_tag="ACHKUI_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin hybrid non-ribosomal peptide
                     synthetase/type I polyketide synthase ClbB"
                     /protein_id="HFO6599431.1"
                     /translation="MDNTSGDFPCNKMDTRKQLPLTPSQQGFLFHSLKDKKRSNYHEH
                     FTCIFSQHVDSAHFKWALETLFRKHECFRTDYNWEIDERPCQVVKTDVLPDIYVLDCE
                     QEEIRFLLANDDIIIPVPQDDGIDAIIPQLLQADLKYPFSLKTIPVRAYLIQSTKESA
                     FILSYHHIVMDGWSLSLFIKQLLQLYGAAVVSGVRDDSAIIPSSLKPLVDTLSARRHT
                     FQHDYWAAYLREGTPTCIVPLSQYHTDTEAENNSYVNQTNHVEINLSPDVCQKIQTLC
                     SDYRITPAVIFYVAWGILLQRWCYADDVLFGATISGRNIPIDGIEETLGLFINTLPLR
                     LRDDGATLLQHLQRMHQTLIAHYSNEHDALASIQRLVHKEGHAGDLFNTLVVLENYPV
                     DMTLLSCASPVAIRHLSVHEQTHYPLTLTITQQKGFRFSIAYALNYLTNNMAQALLMH
                     LSYLLEQLVDNPQRPIAALVNLSPCQQAQVLQPYLERMACRDWDSQSNVIEQFHQVAA
                     TSPAQVAVVDELCALTYSELAAQAEQLAAYLVQQGVMVGDTVGIISERRVNTVVAIIA
                     IMLIGAAYVPISPDYPVGRMQEIIDDSGLALLLVHGKPLDALNVAQSDLCAFPVAPSV
                     VFPVITPDSRAYVIYSSGSTGKPKGIAVAHRGLLRLIQGDSPLKVESGETTLLTCPFE
                     FDVSVFEMWSTLLNHGKLVLLSKQALLDINHIRRTIADEQVARAWFTSSLFNSYVAEG
                     ADFFGMLQHITVGGEAVSAWHVNDVMQKYPHLVVTNGYGPTENTIFTTAYRFNGLQPA
                     RVPIGYAVPGTSLYITDLHGHLLPIGATGELVAGGVGVAIGYQNNPALSATVFVPDPF
                     IPGGMMYKTGDYARLLDDGCVDCFGRKDGQIKINGQRIETGEIEQRLLECSGIIEAVV
                     VPYRVRETLHIAAVVCVNDSYDEVEVRGQLADRLPPFAIPESLVVVTEIAKSHSGKAD
                     LAQLRYLLPATQCNAVSTTISEVHSDMEHALHAIWQRVLDRQDIDSNASFFALGGTSL
                     DTIRVKGDIKRQLGLEIDITDLFKYPTLTALAHFLDTAVSPEDAIPTRAVVYSDMPVA
                     IVGMAGRFPGAANIAALWTLVVGGESGLTLFSDEELRAHGVTPDTLKQANYIKTKGIV
                     DDHEWFDADFFGYTPNEAECMDPQIRLLHQCCWQTLEHAGCDPATFTGAIGIYAGLLT
                     SPHWLNAVMQDTTDSTALYKASILNIHSVTALIAHALNLTGPAVTLDTACSTSAVAIH
                     QACIALRNRDCDAALAGGVSIEMPAYRGYEYHEGMINARDGVCRPFDSQASGTVTGDG
                     LGMLLLKRLDDALADRDCIYGVIKGSAVNNDGNNKIGYTAPSVIGQSTVIRTSLRRAG
                     FDSDSIGLVEAHGTGTVLGDPIELRALNEVFGPTPVPFCVVSALKSNIGHLNSAAGVA
                     GVIKTTLALHHQVLPPTAHFRQLNPAIDLSRSALYVNQQVQPWPSTRPRRALVSSFGI
                     GGTNASIALEAHQHEDDPSATGVRDSYLLLFSAKTPAALELRVASTLEYVKHGVGVRL
                     PDVAYTLQTGRTAFDHRRAYLVSRGSKIDLSCATILQAEIFNGQRTTAEICFMFPGQG
                     SQYHGMASALYAHQPMFRQHMDRCFAAFQRYSTVDLKALLFDDEDTRDIDQTQFTQPA
                     LFCVEYSLARTLIDLGITPDSMIGHSLGEYVAACIAGVFTLEDALHVIEARGRLMQSM
                     RPGSMMAVYLSREQLTPWLAAERGIELAANNSAHFCVVAGEQAAISRLSTRLVEGGIQ
                     HRRLKTSHAFHSAMMTPMLHDFAQLLGQIPMHAPHKRFISNVSGTWITEEQATSPDYW
                     VQQVRNAVLFSEGAAQLLVQPTLFIECGPGNTLSTFIQGHNQYSDQPTLLTLRKANAA
                     IDDEHMLHRTLAALWVRGENIDWRRFNQTALGKHIPLPDYPFEQTYYYRYGAALSGYR
                     QYPNPLRRPQDEWLQRVLWRMHDTSLREAFYAPGELIIIISADGDKLQQTLMSSGVDS
                     ITMPLPISSEDDVWDNDRILTHFHDICALLAHKTYRQLHCLYAPGAEAGSSLTQSLSG
                     LYRVARWCMHSTTPLASLTVLTHGAFRVQEEDNPEPTLAALSGAVNVFAQELHPTEVR
                     LIDIDAQSSDENLNLLTQRLAPKQETVMALRQGMLYLRRFIPTRLLAHLPPQTGCIPG
                     NVLWIIGGEKGIGRMIGEALAQREGVRVVLSSRTGYHHEAVQQDALDVIHCDVTQAEA
                     VRACLATLLERYGRLDGVIFAADATTTLTLHQLSESALRDTLTVKERGTANVLHALAQ
                     RNLLDERLLLLFCNSLAAVNAEIGQTGYATASAYLDALAQQLRTRYKVNALSIGLDAL
                     REQGMLLDAINGSEYDVLRGLRPLMTGTLLQAYKQQGADTSYYARLSPESDWLLDEHR
                     ISGIATLPGTGYLALAYEALRHYFVQDQICIDELVFLAPLTVMDNCSVDVFVDISPNG
                     QGVSVEVKSMTERFSGTLTTHARGRATRLMVDDNVVCDLTGLMREMHTITPPTKELSS
                     THFHYGPRWHSVQQLYGNTAQTQVFATLALPTVAANDTIALHPALLDIASSVVEQLPG
                     FHTDSVPFLYQDLRLYRPLPNTLHVALTVNRHDEEGDSYAFTLYDMAGEMVARCAAMV
                     KRKVQLHIQDVDDDTRLRVPSADNYQLRLAAEGEGAGKLALCPTPRLALGDSQVEIEV
                     LATGLNFKDVLFTTGLLRQQPGEAPLQLGLECAGRITRVGKNVTEFAPGEDVMAVLNG
                     GFVQYARVESDCVVRKPAHCRIEQAAALPIAYLTAYYALVVRANLQPGERVLIHSAAG
                     GVGLAALHIAKRCGAQIFATAGSEQKRDYLLSLGVHAVADSHDEQFAATLLTASDGQG
                     MDVILNSLTGRLLDASLALLAPLGRFLELGSKDIVEDKALPMRFFAQGGTFIPINFHA
                     AHGAFSRYLQQIVAWIDDNTLPLLPCKSVPLPEVARAFATLTTPQHIGKVVVTHRTAA
                     GMDRLNAMIAERRLGGYALSMSNAEVMRQLWPILNTRSPWAQLLLSPRAIDRLARGNR
                     VDRGVPSAANDTITQQTVKKRPRPEIGVPYSPATREVERVLCQILEEYLGLDRVGIDD
                     NYAELGATSLDMVQLSGQMARHYPQVSVVSLYNHATVRQLATFCQPPEGESNAPSPQP
                     AVQTNTRANQIAKRALQIAKNTARSHTSLH"
     gene            9795..12395
                     /gene="clbC"
                     /locus_tag="ACHKUI_003285"
     CDS             9795..12395
                     /gene="clbC"
                     /locus_tag="ACHKUI_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbC"
                     /protein_id="HFO6599432.1"
                     /translation="MEYASEMNGMEIAIIGMAVRFPQSRTLHEFWHNIVQGKECVTFF
                     SEEELLAEGVEQSTLDNPAYVRAKPYIEGICDFDAAFFGYSHKEAQTLDPKSRVLHEV
                     AYHALEDAGYAQRTSDLITGVFVGASEDVDWLRRSLSQIGGDALNRFESGIYGHKDLL
                     AHLIAYSLNLNGPVYSLYTSCSTSLSATHIACRSLLFGECDLALAGGITIDLPQKSGY
                     FCQQGMIHSTDGHCRPFDSQASGTLFGDGAGVVVLRRLEDALAAGDRIYAVIRGSAVN
                     NDGKQKIGFVAPGHEGQKAVICAACHLAEVSPESIGYVETHGTGTRIGDPIEFAALTE
                     AFDTSHRQYCALGAVKANIGHTHAAAGVAGLIKTALVLHHRTIPPLANYQMPNSKLDL
                     AHSPFYIPIQPQEWPASRMPPRAGVSSFGIGGTNVHMILEGLNPAVRDDHDQVRAPVF
                     IPLSAPSFEQLDELTQQLTPLLATLDASTLAYTQQVARPVFDCRRVIQVENDGTQAML
                     ASLDNLMPDAPWGLHCPDLRTTNDCTYAQWLAHSAHYQREATALTALLDGMNIPPAYC
                     HAETWAAQANSSLLIRGCQTIAALKTWMNLLPTLTLLSGAGTGLLPAAAASGMIATQD
                     VLHLLWEMEQKALHLWLPERHEPIPGYVLAWQGNPITDAQRNDRGFWSEALLADTREL
                     GEGVHSINWVRLPPEIREDVDVLRYVAQLWCAGINVDWAVWYGTPLPQRGSASAYPFA
                     HNHYPLPGRVMGSVETQPEAGPETHHPYQARPVLSVPFVAAHSRGMQYITGLMELLLE
                     ISPVGVDDDFFELGGHSLLVTQLTSRLERDFNVHIDLLTLMENPNPRNIYAHIAAQLG
                     GEDNLEIACQ"
     gene            12405..13274
                     /gene="clbD"
                     /locus_tag="ACHKUI_003286"
     CDS             12405..13274
                     /gene="clbD"
                     /locus_tag="ACHKUI_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbD"
                     /protein_id="HFO6599433.1"
                     /translation="MMNVAVIGAGVMGTGVAHNMAQYGISTNVVDISQSQLDKCRQMI
                     EANLRLYNFHPQHKKKTHSTAEIMENIRFTTELDDIVECDLVIENITEDIEKKNALYT
                     RMNTICGASTVFGVNTSAISITALSKLMRHPENVVGVHFMNPVPLMHTVELIRGVHTA
                     ERTLNIFHHLFAQLNKTGIVVNDSPGFVTNRAMMIFVNEAIFMVQEQIARAEDIDTLF
                     KTCFGHKMGPLQTADLIGLDTILQSLQVLYESFNDDKYRPSFLLKKMVDAGYLGVKSG
                     QGFYRYQQTYAEQ"
     gene            13304..13552
                     /gene="clbE"
                     /locus_tag="ACHKUI_003287"
     CDS             13304..13552
                     /gene="clbE"
                     /locus_tag="ACHKUI_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis aminomalonyl-acyl
                     carrier protein ClbE"
                     /protein_id="HFO6599434.1"
                     /translation="MKKQDMKAAIREFLSRSLRGHTLNDDDDIFSLGLVHSLFTVQII
                     LFIEKNFQVELEVSELKTEQIATVNKIVELIQRQTGLE"
     gene            13556..14686
                     /gene="clbF"
                     /locus_tag="ACHKUI_003288"
     CDS             13556..14686
                     /gene="clbF"
                     /locus_tag="ACHKUI_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbF"
                     /protein_id="HFO6599435.1"
                     /translation="MCTENYELAQQEAVLFAKQHLALAAQNIERQQFIVPDIISCVAQ
                     AGYLGASIPQKYGGRGYDSYQLCALHEVMAGVHGSLENLITVTGMVSTLLQRVGSAAQ
                     KAHYLPKLATGELIGAIALTEPNIGSDLVNVETELQQDGDGWRLNGKKKWITLGQIAD
                     FFIVLIHCGNQLATVLIDRNTDGFTITPLNDMLGLRGNMLAELHFNDCRLKEDALLGP
                     LTPGVPLAVNFALNEGRFTTACGSLGLCRAAVDVAARYIRQRKQFKRRLFSHGIVQHL
                     FATMLTQTRSAQLMCFSAAEYRETLHPAMINQILMAKYVASKAAVDVAGKAVQLLGAN
                     GCHADYAVERYYRDAKIMEIIEGTSQIHEIQIAMNYMMGSEA"
     gene            14683..15951
                     /gene="clbG"
                     /locus_tag="ACHKUI_003289"
     CDS             14683..15951
                     /gene="clbG"
                     /locus_tag="ACHKUI_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis acyltransferase ClbG"
                     /protein_id="HFO6599436.1"
                     /translation="MTKDVALMFPGSGSQYVGMARWLYERYPQVRTLFDEASQITERD
                     MAALCLSGTLVQLAEPTAMALAIYTTSVAHFVAWQQFLAQTGVHVNLRYMLGHSLGEY
                     AALTCSGALSFSQALALVAMRSRLASEIAREMDASTTIIKQGNQALVAAACEVAERET
                     RQQVGIACFNSPQQFMLSGQNSAIIAAEQYLLDHDRQVEVVPLIGGVPYHSPLLKPCG
                     QQLRKALDRCEWRRPCCPVISNVNAQPYPDTVTPVQWLEQQLSQPVQWQRSLTYLTGH
                     LSPIAIEIGPQSVLKNLLLENRYPAPVYAFDNRHDRAQLALVLGDNMAVKTDPEAVRR
                     QRITLLTNALTATRHHRAADVAASAQLKELLSRFFERIQQIEQRGTSSEEDIAFLHEL
                     LEQGFQLKGSSQAEIDACHARLASDNGGQA"
     gene            15999..20795
                     /gene="clbH"
                     /locus_tag="ACHKUI_003290"
     CDS             15999..20795
                     /gene="clbH"
                     /locus_tag="ACHKUI_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbH"
                     /protein_id="HFO6599437.1"
                     /translation="MEQQGIMRQLPTDDQTIVDYLYRIAGEYGEKAAVLMGDAALSYH
                     DLNARSNQLAHYLRGLGIGEDRVVAIRLPRGMAMLIAIFAIVKAGGAYLPLAYNAPRS
                     RIENILSNSGAVCLIGTDDGDRWPIPRVEIDSAAVSAMPTTDLRYRPHARQLAYIIYT
                     SGSTGVPKGVATEHAALLNRIVWMQNAYPISSQDVLFQKTVYTFDVSVWEMFWWAMYG
                     ASVVLLPSGLESDPRTLARLIQRHRVSVVHFVPSMLNLFVEYLEMKQDPRLTASLRLV
                     FSSGEKLTVHSVARFYQSVAQGDLINLYGPTEAAIDVSHHRCLRGYDYDDIPIGQAID
                     GCRLYVLDDHGNPVADGEEGELYLAGIGLARGYLNNVALTDRCFTIHPTLRHLGKPER
                     LYKTGDLVWRDGESQQIHYIGRNDFQIKIRGLRVELGEIEAHAMRFPGVQQAVVVADQ
                     DDPDNQLIYAFVVSSVPLNLAALMDALSKNLPAYMLPNRLLAMSELPLSDNGKCCRKT
                     LLDLARAYSASRVDLRETPAVRYLPLSSAQSSMWFMQQLAPHTALYNNPTALLLEGEL
                     DRTRMDGAIRQLMSRHTLLRAMAETHNGQPVLAVPQCVSSQALLTIVPLPSVSDDNAL
                     QAMINQRAAHPMPLTSGTPLCRFELLTLDDDRSVLLIHLHHIISDGWSKGVLLRELQA
                     AYNGESLTPEPLLEYADYMEYQEEWRQSDAYQDAMRYWQNTLAGTLPILDIPTDQPRQ
                     KVARYQGAFVAFALSANTCERVLAAARAQRVSLYNYLLTAFVLLLHRNARQQEYIVGM
                     PIAARLTKEQEHMIAPLVNVLPLRLPLDEAASFSELVQTIRGILFAAFRHQRLEFTDI
                     VRAVNVDRSAGHFPIYQCMFQLDNMPLASPTLNGVNVTPLLLDTSASQVDISLSMQHI
                     DGRITGTFEYDAGLYSADRIQHLVAQWRELLDEASSQPTQLVRDLIRFTPREHAWLAR
                     HNATEVALPPVDNLLALVLPHCQQRPTQVALRHADDAMTYGELQQATMQMCTWLRAQG
                     VKRGESVALQLPFCFELIIAQLAILSLGASYVPLDGNAPAARNALILAQATPCMLLVA
                     QPLESPHGLTIPWVLVPDWRSLLTEIPNLPVSVAPDALDCDAVVIFTSGTTGQPKGVR
                     LSQRNLVNLTASFISSYQVTHQDVLLPITSVASASFVGEVLPLLAAGGTLVLAQKAQS
                     LDSDALIALLASQRVTILSTTPSLSASLSVLAQSMGSLRLFLCGGEALEYEQIAPLLP
                     HMAVVNGYGLTESGICSTYFPVAKRREQETGALPIGRPIQNTQAYVVDAYNRLVPPGA
                     CGELCFSGLGISPGYLDARQDPERFVELPEYPGVRVLKTGDRARWATDGMLFYLGRQD
                     RQVQIRGYRVELGDIESLLKQHPDIADAWVDVRRNAAATPLLVAFYCSVNGVALDAQQ
                     LRVWLSLRLPLHMLPLLYVPLSAMPLGVNGKIDPQCLPLVDLRQLEGPGEYVPPATEL
                     EQRLAEIWQQLLGLERVGTTTNFFDLGGHSLLLVQMQQYIGQQCGQHVALVDLLRFTT
                     IKRLAEFLLAPDAAQGTTGDQTQLRAAKQRLAFGHTRWAATTDSHH"
     gene            20845..23877
                     /gene="clbI"
                     /locus_tag="ACHKUI_003291"
     CDS             20845..23877
                     /gene="clbI"
                     /locus_tag="ACHKUI_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbI"
                     /protein_id="HFO6599438.1"
                     /translation="MAENDFGIAIIGMAGRFPQADTVQAFWENLLASRECISFYSDEE
                     LLAMGISPEFVQHPDYVKAKGEVADIDKFDAAFFGIAPREAELMDPQHRVLLETAWAA
                     FEDAGYVAADYPGDVGIFAGKSMDSYLMLNLMPHFKRVFSSGSLQAAIGNDKDSITTT
                     IAYHLNLRGPAITVQTSSSTSLVAVCVACQSLLTWQCDMAIAGGVTLGPPAKTGYLSQ
                     EGGITAADGHCRAFSDNSSGFVPGTGAGLVVLKRVDEALRDGDNIYAVIKGFAVNNDG
                     SEKISYTAPSVDAQARAIAQAQRLAGLTPQDITYVEAHGTGTRLGDPVEFSALSQAFA
                     GASQKQYCALGSVKTNIGHLDTAAGVAGLIKTALAVQQGIIPATLHFERPNAQIDLTN
                     SPFYINTTCQPWQPESGIRRAGVTSLGMGGTNAHVVLEQAPAVDLQARAPVPAYSILP
                     FSAKTDSALSSGLARFADFLQHESLPDRRDLAWTLSQGRKAFAHRAALVTRDLHAAGT
                     LLQQAATAPFARGVAQTQLGLGLLFSGQGSQYQRMGHQLYQVWPAYADAFDRCATLLE
                     REYQLDIRHELFRAEVSLAQGERLAQTCLTQPLLFSVEYALAQLWLSWGITPTVMIGH
                     SLGEWVAATLAGVFSLEDALRLVARRAELMHQAPSGAMLMVALPEAQIRALITAPLAI
                     AAVNAPDYSVIAGPTSEILAVSQRLTEQNIINKRLHTSHAFHSSMMQDAAQALRQAFE
                     NVRLNPPTLTIISTVTGAHVSADTLTTPDYWIEQMLMPVQFSAALQEAQATFDVDFLE
                     IGPGATLTQLTNGHALGDRLAFSSLPAGARSSDEHKHILDTVAALWVRGHNIDLSAFA
                     GEQPRRVSLPTYAFDKIRYWVDSPEEQRSAVTPVADAGSVIPSEPSVRRQPRPAFSVP
                     YAAPESKTQRGLVAICEALLGIDGLGIDDNFFEAGGHSLMLGMLLAQVQERFAVTLSF
                     FDVMEDASVRALAQLVEQEQQDDGGSALAVLVNDMINE"
     gene            23921..>28578
                     /gene="clbJ"
                     /locus_tag="ACHKUI_003292"
     CDS             23921..>28578
                     /gene="clbJ"
                     /locus_tag="ACHKUI_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbJ"
                     /protein_id="HFO6599439.1"
                     /translation="MTIHHAALARMLPAEKKEKLLRQLAQSGVSPSRIPIIKADPAQA
                     IPLSFNQERLWFLQKYDSTATNYNLYVVYRLHGVVDMPMLTEALRHVQARHAILRTRI
                     IVRNDRPCQVIDDASSLVLDTVTLAAQAPTSALDAVIQQVINTRFDLARGPLWGVTQI
                     IQPDQGCHLVFCAHHIIIDGISLRLLFDELQQQYARLHAGNETSLPPPPLQYADYAFW
                     QREWFQDTLLANELAYWRARLQDAPLLSTFPSLHPRPAQPSTHGSRFSITLDETLSLA
                     LKHVARTQETTPFVLMLTAFQLVLMRYAQQQRLVIGMPVSGRIRPELQSSIGYYASTA
                     VIYTDFNGVEVGREALQRVKASVKETQGRQQLPFENLVNMLDLPRSLSHSPLFQILYI
                     YHNHVTPRAFTLAGAYWEQVTYHNQTVKYDMTVEVFQNDATFDVSFEYDLGLYDADVV
                     KQIAEALRQHCLSLTSSLETPIGAIPLHAPETATPRRDPLNATNVPWLGPQDVLRIIE
                     QRCVQHPKQLAIQQHDGTLTYAELWARVQFIAMRFRAHGIQPGDRIGVLLPRHRDVIA
                     TMLATWFVGACYVPFDIHQPAARLQRLMQRARLVCLVVRQPGEWGEIVQLSLPELMQD
                     MSNAIRYSTPCALLPDMQAYLLFTSGSTGEPKGVCVVHRGLLNLLLDMQRTFAVGSQD
                     RLLSVTTPTFDISFLEYLLPLISGASLYLTEAERAADSFRMIPLIADYRPTLMQATPS
                     FWHGLLMAGWRGDPELCVLAGGEALPTKVAEELLRCCGSLWNLYGPTETTIWSLKSQI
                     TQAENITLGAPIANTRIYILDNEGHPVPQGVDGELYIAGDGVAQGYDGQPELNAQFFL
                     SEPGVPGGRMFRTGDLVRSDAQGQLFFVGRKDSQIKLRGYRIELGEIERTLARHPHVD
                     AAVVACIERAPLHKALAAFIITSEPPSLFEQLKNELRQQLPDYMVPTLWQRVADFPNT
                     DNGKIDRKRLAENFVADSSLVSPQTQALSDTEQMLLALWMRYLPIKNVDPECDFFRLG
                     GHSLLAVTLVAEINRTFHCALTLKDIFHYSTLRALSARIAQQSITDAAASQDDWVIVH
                     DPEHRHQPFPLTDVQRAYWLGRQTGATSIATHIYHEFDVEHFNVTRFTHAVNALIARH
                     EMLRARVLPDGTQQILAQVPAYQLEQRDLSALSPNARNDALMAIRDRLSHHVHPADRW
                     PLFDFSYSACTAQHGRLHFSLDLLIADALSMRTLQQELMMLYREPHVSLPLLPFSFRD
                     YVQALLVEQASEAYARDQAYWQRALPQLYGPPTLPVQGDLAQLSAIRFVRRRHRLSAH
                     NWGVLSALAQRTRITKTALLLTVFSQVLARWSLSPTFTLNLTLFNRPQGYPNAEAVIG
                     DFTAVSLLNVCYDSQHSYAHNAQRIQVQLWEDLEHRRFSGIRASEALIHSGRFHAPMP
                     VVFTSMLDIDGETTAQDPRDTTRFTLCPDANITQTPQVWLDHQVIELAGELHFNWDAV
                     EQLFDTTLLDQMFGAYCHALQALVAMPQSWWGVNSSLALPTVSAPVTQAPAPTALLHH
                     G"
BASE COUNT         6381 a         7418 c         8043 g         6736 t
ORIGIN      
        1 ggggtattcc tgtaagtgat aaaatgcaat atgattatat gaatacgcaa taaaaaacta
       61 tacttgcgga taggtgtgcc atttagaata atcatgttaa ataatctata aatccgataa
      121 taaggtgatg gttatggata atacctctgg agattttcca tgtaataaga tggacacgcg
      181 taagcagtta ccgctaacac caagtcaaca ggggttttta ttccattcct taaaggataa
      241 gaaaaggagt aactaccatg agcattttac atgcattttt tctcagcatg tagatagcgc
      301 ccacttcaag tgggcgctgg aaacgttatt tcgaaagcat gagtgttttc gcactgatta
      361 taactgggag attgatgagc gcccttgtca ggtggtgaag accgatgtgt tgccggatat
      421 atatgtgtta gactgtgagc aagaggaaat acgttttcta ctagctaatg atgacattat
      481 cattcctgtc ccgcaggatg acggtattga tgctataatt cctcaactgc tacaggctga
      541 tttaaaatac ccattttcct tgaaaacgat cccagtccgg gcctacctta ttcagtcaac
      601 gaaagaaagt gcttttatac tatcatacca tcatattgtg atggatggct ggagcttatc
      661 ccttttcatt aaacagttgc tccaactcta tggagcggct gtggtcagtg gtgtgaggga
      721 tgatagcgcc attatcccct catctctgaa accccttgta gacacactgt cggcccgacg
      781 tcacaccttt cagcacgact attgggctgc atatcttcgg gagggaacac caacttgtat
      841 cgtgccgctg tcacaatatc acacagatac tgaagccgag aacaattctt acgttaatca
      901 aacaaatcat gtggagatca atttgtctcc ggatgtgtgt cagaaaatac agacgctatg
      961 cagcgattat cgtatcaccc ccgcagtaat cttctatgtg gcctggggca tcctgctaca
     1021 acgttggtgc tatgctgacg atgtgttatt cggcgcgaca atatcagggc gaaatatacc
     1081 aattgatggt atagaagaaa cactagggct atttattaac acgttgccgc tgcgtctgcg
     1141 tgatgatggg gcgacactgt tgcaacactt acaacgtatg caccaaacac taatagctca
     1201 ctacagcaat gaacatgatg ccttagccag catacaacgg ttggtacata aagaaggtca
     1261 tgctggggat ctttttaata ccttagtggt gttggagaat tatcccgttg atatgacatt
     1321 attgtcatgc gcgtcgcccg tggcaattcg ccatctcagt gtacatgaac aaacgcacta
     1381 tccgctgacc ttgaccatca ctcaacagaa gggattccgt ttcagtattg catatgccct
     1441 taactacctg accaacaaca tggcgcaagc gttgctgatg cacctgagtt atctgcttga
     1501 acaactggtg gacaatccgc agcgccccat tgctgcgttg gtaaacttgt ccccatgcca
     1561 gcaggcgcag gtacttcaac cctatctgga acgcatggca tgccgggatt gggatagtca
     1621 atccaacgtc atcgaacaat ttcatcaagt tgctgcgact tcgccagcac aggtcgcagt
     1681 ggttgatgaa ttgtgcgcgt tgacctattc ggagttggca gcacaagctg agcagctcgc
     1741 ggcttatttg gtacagcagg gtgttatggt tggcgatact gtaggcataa ttagcgaacg
     1801 ccgggtaaac accgtggttg ccatcatcgc catcatgttg atcggggctg cttatgtgcc
     1861 catcagcccc gactacccag tgggtaggat gcaggaaatt attgatgaca gcggcttggc
     1921 gctgttgctg gtacatggca aaccgctaga tgcattaaac gttgcgcaga gtgacctctg
     1981 tgcatttccc gtcgcgccct cggtggtatt tccggttatc acaccagatt ctcgcgctta
     2041 tgtgatttat tcatcggggt cgacgggtaa gccaaaaggt atcgcggtgg cgcaccgcgg
     2101 tttattgcgc ctgatacaag gcgacagccc gctgaaggtg gagagcggtg agacaacgct
     2161 gctgacctgc ccatttgagt ttgatgtttc ggtgtttgag atgtggtcta ccttgctcaa
     2221 ccacggcaaa ctagtattac tcagcaaaca ggcattactt gatatcaatc acattcgccg
     2281 cacgatcgct gatgaacagg tggcgcgcgc ctggtttacc tcatccttgt ttaactccta
     2341 tgtggcagaa ggtgccgatt tcttcggtat gttacaacac attacggtgg gcggcgaagc
     2401 agtatctgcg tggcatgtca acgacgtgat gcaaaaatac ccgcatctgg tggtgacaaa
     2461 cggttatggg ccgacggaaa acactatttt taccaccgca tatcgtttca acgggttgca
     2521 acccgcccga gtcccgatag gatacgcggt accgggcacc tcgctctaca ttaccgatct
     2581 ccatgggcat ttgttgccta tcggtgccac cggtgaacta gtggcgggtg gagtgggggt
     2641 cgccatcggc tatcagaaca acccggcgct aagtgcgacg gtttttgtcc ctgatccttt
     2701 tattcccggc ggtatgatgt acaaaactgg cgattatgcc cggttgctgg atgatggctg
     2761 tgttgactgt tttgggcgta aagacggtca aattaagatc aatggacaac gaattgaaac
     2821 cggagagatc gagcagcgcc tgctggagtg ctccggcatt atcgaggcgg tagtggttcc
     2881 ttaccgcgta cgtgaaacgc tgcatattgc ggcagtggtc tgcgtcaatg atagctatga
     2941 tgaggtggaa gttcgagggc aattggctga cagattgccg ccattcgcta ttccggaatc
     3001 cttggtggtg gtgacggaga ttgcaaaaag ccatagtggc aaggccgact tggcgcagtt
     3061 gcggtatctc ctgcccgcaa ctcagtgcaa cgccgtgtcc accacgatat cagaggtgca
     3121 tagtgacatg gaacatgcgc tgcatgctat ctggcaacgc gtacttgata gacaagacat
     3181 tgatagcaat gcctcctttt tcgccctcgg tggcacctca ttggatacca tcagggttaa
     3241 aggggatatt aagcggcaac ttggcttgga gattgatatt accgatctct ttaagtaccc
     3301 aacgctcacg gcgttagcac attttctcga tactgccgta tcgccggagg atgcaattcc
     3361 aacgcgtgct gttgtctaca gcgacatgcc ggtggcgatt gtcggtatgg cgggacgttt
     3421 ccccggtgcg gcgaatattg cagcgttgtg gacgctggtt gtaggagggg aatcgggatt
     3481 aacactgttc agtgatgaag agttgcgcgc gcatggtgtg acacctgaca cgcttaaaca
     3541 agcgaattat ataaaaacca aagggattgt tgatgatcac gaatggtttg atgcggattt
     3601 ctttggttat actcccaacg aggcggaatg tatggatccg caaattcgct tattgcatca
     3661 gtgctgctgg caaactctgg aacacgctgg gtgcgatcct gccaccttta ctggtgcgat
     3721 tggcatttat gccggactgc tgacatcccc ccactggctt aatgcggtaa tgcaagacac
     3781 taccgactct accgccctgt acaaggccag tatcctgaat atccattccg tcacagcatt
     3841 gattgcccat gcgcttaacc tcaccggccc tgccgtgacg cttgacaccg cctgctccac
     3901 ttcggcagtg gctatccacc aggcctgcat cgcactacgt aaccgggatt gcgatgcggc
     3961 attggcgggc ggcgtttcta tcgagatgcc tgcgtaccgg ggctatgaat atcatgaagg
     4021 catgatcaat gcgcgagatg gtgtctgtcg tcctttcgat agccaagcct ctggcacagt
     4081 caccggtgat ggcttaggaa tgctgctgct aaaacgtctg gacgacgcgc tggcggatcg
     4141 ggactgcatc tatggtgtga tcaaaggttc ggcggtcaat aatgatggca ataacaaaat
     4201 cggctatacc gcgccgagtg tgatcgggca atcaacggta atccgcacca gtttgcgccg
     4261 tgccggtttt gatagcgaca gtatcggatt ggtggaagcg cacggcaccg gtacggtgct
     4321 gggtgatcct attgagttac gtgcgcttaa tgaggtgttt ggcccaacac ctgttccgtt
     4381 ttgtgtggtt tcagcgctga aaagtaatat tggccacctc aactccgctg cgggagtggc
     4441 gggcgtcatc aaaacaacgc tcgccctgca tcatcaagtg ttgccaccaa cagcgcactt
     4501 tcgccaactc aatcctgcta tagatctatc gcgttctgca ttgtacgtta accagcaagt
     4561 ccaaccgtgg ccgtcgacgc gtcctcgtcg tgcactggtg agctccttcg gcatcggcgg
     4621 caccaatgcc agcatcgcac tagaagcaca tcaacatgag gacgaccctt cagcgacggg
     4681 ggtacgcgac agctatctgt tgttgttctc cgctaaaaca cccgctgcgt tagagttgcg
     4741 cgtggcctcc acactggaat atgtcaagca tggagtaggg gtgcgcctgc cggatgtggc
     4801 ttatacattg caaactggac gcacagcctt tgaccatcgg cgggcttatt tggtgagtcg
     4861 tgggtcgaaa atcgatctct cctgtgccac gatattgcaa gcggaaatct tcaatggtca
     4921 gcgcacgaca gcggagatct gcttcatgtt tcctggtcaa ggtagccaat atcacggcat
     4981 ggccagcgcg ctctatgctc atcaacccat gtttcgccag cacatggatc gctgctttgc
     5041 tgcattccaa cgctattcga cggtcgatct caaggcgttg ttgtttgacg atgaggatac
     5101 gcgggatatt gatcaaacgc agttcacaca accggcgttg ttctgtgtcg aatacagcct
     5161 agcgcgcacc ttgattgatc tggggattac gccggacagt atgatcgggc acagtctggg
     5221 cgagtatgtt gcggcctgta ttgctggcgt atttactctt gaggatgcgc tgcacgtcat
     5281 tgaggcgcgc ggacgtttga tgcagtccat gcgtcccggt agcatgatgg cggtctacct
     5341 tagtcgcgaa cagttgaccc catggctagc tgcagaacgg ggtattgaac tggcagctaa
     5401 taatagcgcg catttttgcg tagtcgcggg cgagcaggcg gccatttccc gtttgagcac
     5461 acgcttagtc gagggcggga tacagcacag gcgcctgaaa acctctcacg cattccactc
     5521 ggccatgatg acgccgatgc tgcacgattt tgcacagttg ctggggcaaa tcccgatgca
     5581 cgcgccgcac aagcgcttta tatctaatgt aagcggtaca tggattactg aggagcaagc
     5641 tacctcgccg gattactggg tgcagcaagt gcgcaacgcg gtgctgttta gcgaaggtgc
     5701 ggcgcaactg ttggtacaac ccacgctgtt tatcgaatgt gggccgggta atacgctctc
     5761 tacctttatt caaggacata accaatacag cgatcagccg accctgttga cgctacgcaa
     5821 agccaacgcg gcgatcgatg atgagcacat gttgcatcgt acgctggcgg cgctgtgggt
     5881 caggggggag aatattgatt ggagacgctt taaccagacg gcactcggca agcacattcc
     5941 attgccggat taccccttcg aacagactta ttactatcgc tatggtgctg cactttccgg
     6001 ttatcgccag tatccaaatc ctctgcgccg tccgcaagat gagtggctcc agcgtgtgct
     6061 gtggcggatg cacgacacat ccttgcggga ggcgttctat gcgccgggcg aattgatcat
     6121 cattattagt gctgacggcg acaagttaca gcagacgctg atgagtagtg gtgtcgacag
     6181 catcacaatg ccgctgccga tatcgtcaga ggacgacgtg tgggataatg accgtattct
     6241 gacgcatttc cacgacatct gcgcactttt agcacacaaa acctaccgac agttacattg
     6301 cttgtatgct cccggtgcgg aggcaggatc atcgttgaca cagtcgctct cgggacttta
     6361 tcgtgttgct cgctggtgta tgcacagcac cacgccgctg gcgtccttaa cagtattgac
     6421 ccatggtgcg tttcgcgtac aggaagagga taatcccgaa ccgacgctgg cagcgttgtc
     6481 gggcgcagta aacgtgtttg cccaagagct gcacccgacc gaagtacgat tgatcgatat
     6541 cgatgcgcag agcagtgatg agaatctgaa cttgctaacc cagcgtctgg ccccgaaaca
     6601 agaaacggta atggcgctca ggcaggggat gctttacctg cgacgcttta tcccgacacg
     6661 gctgttggcg cacttacccc ctcaaacagg gtgtataccg ggcaacgtac tgtggattat
     6721 cggcggtgaa aaggggattg gccgcatgat cggcgaagcg cttgctcagc gtgagggagt
     6781 ccgtgtggta ctgagcagcc gcacaggtta tcaccatgaa gcggtgcagc aggacgcatt
     6841 agacgttatc cactgcgacg tgacgcaggc ggaggcggtt agagcttgtc tggcaactct
     6901 gctcgaacgc tatggacggt tggatggcgt gatttttgct gccgacgcta ccaccacatt
     6961 gacactgcat caattgagcg aatctgcgct acgcgacacg ctaacggtga aagaacgggg
     7021 tactgctaat gtgctgcatg cgttagcgca acggaatctg ctggatgagc gtctgctact
     7081 gctgttctgt aactcgttgg ctgccgtgaa tgcggagatt ggccagacag gctatgctac
     7141 cgccagcgcc tatttggatg cactggcaca gcaactgcgt acccgctaca aggtgaatgc
     7201 gctcagtatc gggttggatg cattgcgtga gcagggcatg ttgttggatg ctataaacgg
     7261 cagtgaatac gatgtcttgc gtggactgcg cccattgatg acgggaacgt tgctacaggc
     7321 ttataaacaa caaggggctg acaccagcta ctacgcgcga ttatcccccg agtccgattg
     7381 gctgctggat gagcaccgga tatccggtat cgccacgctg ccgggaaccg gctatctggc
     7441 gctggcgtat gaagctctgc gccattactt tgtgcaagac caaatctgca ttgatgaatt
     7501 ggtctttttg gcaccgttga ctgtgatgga caactgcagt gttgacgttt ttgttgacat
     7561 ttcacctaac gggcaaggag ttagtgtcga ggtgaaatca atgacggagc gctttagtgg
     7621 cacgttaaca acccatgcca gaggcagggc gacgcgtctg atggtagacg ataatgttgt
     7681 gtgcgatctc acggggctga tgcgcgagat gcacactatc actcctccaa caaaggaatt
     7741 atcgagcacg cacttccact atgggccgcg ctggcacagc gtacaacaac tgtatggcaa
     7801 taccgcccag actcaggttt tcgcaacgct ggccctgccc accgttgccg ctaatgatac
     7861 gatcgcactg caccctgcgc tgttggacat cgccagcagt gttgtcgaac aactgcctgg
     7921 ttttcatact gattcggtac ctttccttta tcaggattta cgcctgtacc gcccgttgcc
     7981 gaacacctta catgtggcgc tgactgtcaa tcggcacgat gaggagggtg acagctacgc
     8041 tttcacgctc tacgacatgg caggcgagat ggttgcccgc tgtgcggcaa tggtgaagcg
     8101 caaggtacag ctccacatac aagatgtcga tgacgacacg cgactgcgcg tgcccagtgc
     8161 cgataactac caactgcggc tggccgctga gggggagggg gcaggaaagc tagcgttgtg
     8221 ccctacgccg cgcttagcgc tgggggattc acaagtagag attgaggtac tggccaccgg
     8281 actgaacttt aaggatgtgc tgttcaccac gggattgctc cggcagcagc cgggtgaggc
     8341 tccgctgcaa ttgggactgg agtgcgccgg acgcattact cgcgtgggta aaaatgtcac
     8401 tgaatttgcc ccgggagagg atgttatggc ggtgctaaac ggtggttttg tccagtacgc
     8461 acgggtagaa agcgattgcg tagtgagaaa accagcccat tgtcgcatcg aacaggcggc
     8521 tgcgctgcct atcgcatacc tcaccgccta ttacgcactg gtggtgcgcg ctaatttgca
     8581 acccggagaa cgagtattga tccacagtgc ggcggggggc gttggcttgg ctgcgctaca
     8641 tattgccaaa cgctgcggag cacagatttt cgccacagca ggtagcgagc agaaacgcga
     8701 ttacttgctt tcgctaggcg tacatgccgt agctgattca cacgacgaac agttcgctgc
     8761 cactctgctg accgcatcgg acggacaggg gatggatgtg atccttaact ccctcacagg
     8821 ccgtctgctt gacgctagcc tcgcgctgct ggcaccgctg ggccgttttc ttgagctggg
     8881 cagcaaggac attgtggaag acaaagcgct accgatgcgt ttcttcgccc aaggcggcac
     8941 ctttattccg attaattttc acgcggcgca tggtgcgttt agtcgctacc tgcaacagat
     9001 tgtcgcttgg atagatgata acacgctacc gctccttcca tgcaaatccg taccattgcc
     9061 cgaggttgca cgcgccttcg ccaccctgac cacgccgcag catattggca aggtggtggt
     9121 aacgcatcgc actgcggcag gcatggaccg gctgaacgcc atgatagcag aaaggcgcct
     9181 cggcggctat gcgctcagca tgagcaatgc cgaggtgatg cgccaattgt ggccgatact
     9241 gaacactcgc agtccgtggg cgcaactgct gctctcacct cgggcgatcg atagattagc
     9301 gcggggcaac cgggtggatc gcggtgtacc gtctgccgct aacgatacga ttactcagca
     9361 gacagtgaaa aagcggcccc gtccggaaat tggcgtgcct tacagccccg cgacacgtga
     9421 agtggaacgc gtgctctgcc aaatcctgga agagtatctg ggtctggata gagtaggcat
     9481 tgacgacaac tatgccgaat taggggcaac ctcactcgat atggtgcaac tgagtgggca
     9541 aatggcgcgt cactatccgc aagtgagcgt ggtgtcgctg tacaaccacg ccaccgtgcg
     9601 ccagctggcg acgttttgcc agcccccgga gggcgagtca aatgcgccat caccacagcc
     9661 tgcagtacag acgaatacgc gcgcgaatca gatagcaaaa cgtgctttgc aaattgcgaa
     9721 aaatactgcg cgcagtcaca cgtctttgca ttaatccggg cgcctcctcg ccctgaagaa
     9781 aggtaatgaa gtttatggaa tacgcaagcg aaatgaacgg catggaaatc gccattattg
     9841 gtatggcggt ccgtttcccg cagtcccgga cgttacacga attctggcat aacatcgttc
     9901 agggtaaaga gtgcgtcacc ttctttagcg aggaggagtt gctggccgaa ggcgtcgaac
     9961 agagtactct ggacaacccg gcctatgtac gggccaagcc ctatatcgag ggcatatgcg
    10021 actttgatgc tgcatttttc ggttacagtc acaaagaggc gcagactctg gatccgaaat
    10081 cccgcgtatt acatgaagtt gcctaccatg cgctggaaga tgcaggctat gcccaacgta
    10141 ccagcgatct gatcaccggg gtattcgtgg gggcgtcaga agatgtggat tggctacggc
    10201 gttcgctgtc acagattggc ggcgatgcgc tgaatcgttt tgagtctggc atctatggtc
    10261 ataaggatct gctcgcacat ctcattgcct acagtctcaa tctcaacggt ccggtgtata
    10321 gtctctacac cagttgttcg acttctctga gtgcaacgca tatcgcttgc cgcagcttgt
    10381 tgtttggcga atgtgatctg gcgttggcgg gtggaattac tatcgattta ccgcagaagt
    10441 caggctactt ctgtcaacag ggcatgatcc actccaccga tggccactgc cgtccttttg
    10501 acagtcaggc ttctggcacc ctgtttggcg atggcgcggg tgtggttgtg cttcggcgtt
    10561 tggaagatgc tctggcagcg ggcgatcgca tctatgcggt gatccggggt agcgcggtca
    10621 acaatgacgg taaacaaaaa atcggttttg tcgcgcctgg tcatgaggga cagaaggcgg
    10681 tcatttgtgc ggcctgtcat ctggcagaag taagcccaga aagcatcggc tacgttgaaa
    10741 cccacggtac cggcacccgt attggcgatc ctatcgagtt cgctgcgttg acggaggcgt
    10801 tcgatacttc acaccgccaa tactgtgcac taggggctgt aaaggcgaat attggccaca
    10861 cccacgcggc ggcgggcgtg gctgggttaa tcaagacggc gctggtactt catcaccgga
    10921 ccattccgcc gctcgccaac tatcaaatgc ctaactcgaa gctggatctg gcgcattcac
    10981 cgttttatat cccgatacag ccgcaggagt ggcccgcatc gcggatgccg ccccgcgctg
    11041 gcgtcagttc ttttggcatt ggcggtacta atgtgcatat gattttggaa gggctgaatc
    11101 ctgcggtgcg cgatgaccat gaccaagtgc gagcaccggt gtttatccct ctctccgcgc
    11161 cgtctttcga gcagttggat gagctgacgc aacagcttac cccgttgctg gctacccttg
    11221 atgcgtcaac gctagcctac acacaacaag tggcgcgccc cgtgtttgat tgccgccgag
    11281 tgatacaagt ggaaaacgat ggtacgcaag cgatgctggc atcgctggat aacctaatgc
    11341 ccgacgctcc ttggggccta cactgtccag atctacgtac tacgaacgat tgtacttacg
    11401 cacagtggct ggcgcattcg gcacattatc aacgcgaagc gactgctctg acggcattac
    11461 tcgacggcat gaatattcca cccgcttatt gccacgctga aacgtgggcg gcacaagcga
    11521 acagcagcct gctaatcaga ggctgccaga ctatcgccgc gctaaaaacc tggatgaacc
    11581 tgctaccgac attaacactg ctgtcgggtg ctggaacagg ccttttgcct gccgctgcag
    11641 ccagtggcat gattgcgacg caagacgtgt tgcatttgct gtgggaaatg gagcaaaagg
    11701 cgcttcatct ctggcttcct gagcgccatg aacctatccc cggctacgtg ctggcttggc
    11761 aaggaaatcc catcaccgat gcacagcgta acgacagagg gttttggagt gaagcgctgt
    11821 tggcagatac ccgagaactg ggggagggcg ttcacagtat caactgggtt aggctgccgc
    11881 cggaaataag agaagacgtt gatgtattgc gctacgtggc gcaactgtgg tgtgcaggta
    11941 tcaatgtgga ttgggccgtg tggtacggca ctccgctgcc gcaacgcggg agcgcatcag
    12001 catatccctt cgcacacaac cactacccat tgcctggacg ggtaatgggt agtgtggaga
    12061 cccaacccga agcaggaccc gaaacgcacc acccttatca ggcacgtccc gtgttaagcg
    12121 tacctttcgt agccgcacac tcgcggggta tgcagtatat cacaggtctg atggaactgt
    12181 tgctggaaat atcgccggtc ggggtggacg acgacttttt tgaattaggc gggcattcgc
    12241 tgctggtgac gcaattgacc tcccgtttag aacgcgattt caacgtacat atcgatcttt
    12301 tgaccctgat ggaaaacccc aatccgcgca atatctacgc gcatatcgcg gcgcaactgg
    12361 ggggcgaaga caacctcgaa atagcctgtc agtaaaggag ctcaatgatg aacgtggcgg
    12421 taataggtgc aggagtaatg ggaactggcg tcgctcataa catggcgcaa tacggcatat
    12481 caactaacgt tgtggatatt tctcaatctc agttggataa atgccgacag atgatcgaag
    12541 cgaacttacg cttatataat tttcacccgc agcataaaaa aaagacgcat agcacagcgg
    12601 agataatgga aaatatccgt ttcacaactg agttggacga cattgtagaa tgtgatttgg
    12661 tgattgagaa tattactgag gatatagaga aaaaaaatgc actatacaca cggatgaata
    12721 cgatctgcgg cgcgtcgaca gtgtttggtg ttaatacgtc cgccatatct atcactgcgt
    12781 tgtcaaaatt aatgcgacat ccggagaatg tagtcggcgt ccattttatg aacccggtac
    12841 cgctgatgca caccgtcgaa ttaatccgtg gtgtacatac tgcagagcgt acactgaaca
    12901 tatttcacca cttatttgct cagttgaata aaaccggcat cgtggtcaat gattctccgg
    12961 gctttgtcac taatcgtgcc atgatgattt ttgttaacga agccatattt atggtgcagg
    13021 agcagatcgc ccgtgctgag gatatcgata cgttgtttaa aacctgcttc gggcacaaga
    13081 tggggccact acaaactgcc gatttgattg gcttggatac gattttgcaa tcgttgcagg
    13141 tgctatatga aagttttaat gatgataagt atcgccccag ctttttatta aagaaaatgg
    13201 tcgatgcagg gtatttgggc gtgaaatcag ggcaggggtt ttatcgctat caacagacgt
    13261 acgccgagca gtgattctgg gttaaccaga tagggatatg aatatgaaaa agcaagatat
    13321 gaaagccgcc attcgggaat ttctttcacg ctcattacgt gggcatacgt tgaacgatga
    13381 tgacgatatt ttttctctcg ggcttgtcca ttcgttattt actgtgcaaa tcatactgtt
    13441 tatagaaaaa aattttcagg ttgagctgga agtgagtgag ttgaaaacag aacagattgc
    13501 taccgtcaat aaaatagtgg agctcattca gcgacaaaca ggcctggagt aaactatgtg
    13561 cactgaaaat tatgagctgg ctcagcaaga ggcagtcctg tttgccaaac aacatctcgc
    13621 cctggctgca cagaatattg aacgtcagca gtttattgtg ccagacatta tttcttgcgt
    13681 ggcgcaggcc gggtacttag gtgcgtcaat cccccaaaaa tatggcggac gaggttacga
    13741 ttcttatcaa ctgtgcgctt tgcatgaagt gatggctggg gttcacggtt cgttagaaaa
    13801 tctcataaca gtgactggca tggtcagcac gctgctgcaa cgcgtgggca gcgcagcgca
    13861 aaaagcccat tatttgccaa agctggcaac gggggaattg atcggcgcga ttgccctcac
    13921 agagccgaat atcggcagtg acttagtgaa cgtggaaaca gaattacagc aggatggcga
    13981 cggttggcgg ctgaacggga aaaagaaatg gatcacgcta gggcagattg ctgatttttt
    14041 tattgtttta atccactgtg gtaatcaatt agcgacagtg cttatcgatc gcaataccga
    14101 cggctttact atcactccac tcaacgacat gttggggtta cgcggcaata tgttggcaga
    14161 gctgcatttt aatgactgcc gcctgaagga ggatgcattg ctcggtccgc tcacccctgg
    14221 ggtaccgctg gcggtgaatt ttgcgctcaa tgaagggcgg ttcaccaccg cttgtggcag
    14281 cctaggatta tgtcgggccg ctgtggatgt ggcagcgcgc tacatccgcc agcgtaaaca
    14341 gttcaagcgg cggttatttt ctcatggcat tgttcagcac ttgtttgcca caatgctgac
    14401 ccaaacgcgc agtgcgcaac taatgtgctt cagcgcggcg gaataccgcg aaacgctgca
    14461 cccggccatg ataaaccaaa tcctgatggc gaagtatgtc gcttccaaag ctgcggtgga
    14521 cgtggccggg aaggcggtgc aactgctcgg tgcgaatggt tgccatgccg attatgccgt
    14581 cgaacgttac taccgcgacg ccaaaatcat ggaaatcatc gaagggacat cgcaaattca
    14641 tgaaatccaa attgcgatga attacatgat ggggagtgaa gcatgacgaa ggatgtcgca
    14701 ctgatgttcc ctggctccgg ttcgcaatat gtaggcatgg cacggtggct gtatgagcgt
    14761 tatccgcagg tgcgtactct gtttgatgaa gctagccaga tcacggaacg ggacatggca
    14821 gcgctgtgcc tgtcgggtac tttggtacaa cttgctgagc cgacggcaat ggcgctggct
    14881 atctatacca ccagcgtggc ccactttgtc gcgtggcagc agtttttggc gcaaaccggt
    14941 gtccatgtca acctacgtta tatgttgggt catagtttgg gcgaatatgc tgcgctgacc
    15001 tgtagtggtg cgctgagctt ttcccaagca ctggcgctgg tggcgatgcg tagccgttta
    15061 gccagtgaga tcgcccgcga aatggacgcg agtaccacta tcatcaagca agggaatcag
    15121 gcactggtgg cagcggcgtg cgaggtggcg gagcgtgaaa cccgacagca ggtcggtatt
    15181 gcctgcttca attcgccgca acagtttatg ttatccggac aaaactcggc cattatcgcc
    15241 gccgagcaat acctgctgga tcatgatcgc caagtcgaag tagtgccgct gattggtggt
    15301 gttccttacc acagcccact attgaaacct tgcggacagc aattgcgcaa ggcgctggat
    15361 cgctgcgaat ggcgacgtcc gtgctgccca gtgatctcca atgtcaacgc tcaaccctat
    15421 cccgatacgg tcacgccagt ccagtggcta gaacaacagc tctctcagcc agtgcagtgg
    15481 cagcgctctc tcacctacct gacaggacac ttgtcaccga tcgcgataga gatcggtccg
    15541 caaagtgtgc tgaaaaacct gctgctggag aatcgctatc cagcaccagt ttacgcattt
    15601 gacaatcgtc atgatcgtgc gcaactagca ttggtgctgg gggataacat ggcggtgaag
    15661 accgatccgg aagccgtgcg ccgccaacgc atcacgctgc tcactaacgc cctgacagcc
    15721 acacgccatc accgcgccgc cgatgtggct gccagtgcgc aattgaaaga attgctcagt
    15781 cgtttttttg agcgcatcca gcagattgag cagcgcggca catccagcga agaggacatt
    15841 gcttttttgc atgagcttct tgaacaggga tttcaactca aaggcagtag tcaggcagaa
    15901 atcgacgcct gccacgcgcg gttggcttcc gacaacggcg gacaggcgta acccgtcacg
    15961 ccattttacg tacttatctc gctttgaagg ccagagttat ggaacagcaa gggattatga
    16021 gacagttgcc taccgacgac caaacgatag tcgactattt gtatcgtatc gccggagaat
    16081 atggggaaaa agccgctgta ttgatggggg acgcggcgct gagctatcac gatcttaatg
    16141 cacgctctaa ccaactggcg cattatctgc gtgggctggg gatcggcgag gatcgtgtgg
    16201 tagctatccg cctgccgcgc ggcatggcaa tgctgattgc cattttcgct attgtaaaag
    16261 ctggtggtgc ctatttaccg ctggcgtaca atgcaccgcg cagccgcatt gaaaatatac
    16321 tgagcaacag cggcgctgtt tgtctgatcg gtactgacga tggtgatcgc tggccgattc
    16381 ctcgcgtcga aatcgacagc gcggcggtca gtgccatgcc aacgacggat ctgcgttacc
    16441 gaccacatgc gcggcaactg gcgtatatta tttacacctc cggttccacc ggtgtcccta
    16501 aaggggtggc gacggaacat gcagcgctgc taaaccgaat tgtctggatg cagaacgctt
    16561 atcctatcag ttctcaggac gtgctgtttc agaagacggt gtacaccttt gacgtctctg
    16621 tctgggagat gttctggtgg gcgatgtacg gcgcatctgt agtgctgtta ccgtccggac
    16681 tggagagcga tccgcgaacc ttggctcgac tgattcagcg tcaccgcgtg tcggttgtgc
    16741 actttgtccc ttcgatgctg aacctgtttg tcgagtatct ggagatgaaa caggatcctc
    16801 gtttgaccgc ctcattgcga ttagtgtttt ccagcggtga gaaactcacg gtccacagtg
    16861 tggctcgctt ttatcaatcg gtggcgcagg gtgatcttat aaatctctat ggcccgaccg
    16921 aagcagcaat cgatgtcagt catcaccgct gcctgcgcgg gtacgactac gacgatatcc
    16981 ctatcggtca agcgattgac ggttgccgac tctatgtgct ggatgaccat ggtaatccgg
    17041 tagcagacgg cgaagagggc gaactgtatc tcgctggtat cgggctggca cgtggctatc
    17101 tcaacaacgt ggcgttaact gatcgctgtt ttactataca tccaactttg cgccatttag
    17161 ggaaaccgga gcggctgtat aaaactggag atctggtgtg gcgcgacggg gaaagccaac
    17221 aaattcatta cattggccgt aatgattttc agattaaaat tagagggttg cgcgttgaat
    17281 tgggagaaat cgaagcccat gcgatgcgtt tcccgggggt acagcaggca gtcgtggtgg
    17341 cggatcagga tgatcccgac aatcagttga tttacgcttt tgtcgtcagt agcgtgccgc
    17401 tcaatttggc ggccttaatg gacgcactgt ccaaaaactt gcctgcctac atgctgccga
    17461 accgtttgtt ggcaatgtca gagttaccac tctccgacaa tggcaagtgc tgtcgtaaaa
    17521 cgttgctcga cttggcgcgg gcgtactcag ccagtcgcgt cgatttacgt gaaactcccg
    17581 ccgtgcgcta cctaccgttg tcatcggctc aatcgtcgat gtggtttatg caacaattgg
    17641 cgccgcatac tgcactatac aataacccca ccgccttgct gcttgaagga gaactggatc
    17701 gcacgcggat ggacggcgcg attcgtcaat tgatgagtcg gcatactctg ttacgtgcta
    17761 tggcggaaac ccacaatgga caaccagtat tggcggtgcc tcagtgcgta tcgtcgcagg
    17821 cgttgctgac catagtgcca ctcccctcgg tgagtgatga taacgcgcta caggcgatga
    17881 tcaaccagcg tgcggcacac cccatgccct taacgtcagg cacaccgctg tgccggtttg
    17941 aactgttgac gctcgatgac gatcgtagcg tattgttgat tcatctgcac cacatcatca
    18001 gtgatggctg gtcgaaaggc gttttgttac gcgaattgca ggccgcatat aacggtgaat
    18061 cgctgacgcc agaaccgttg ttggagtatg ccgactacat ggagtaccaa gaagagtggc
    18121 gtcagagtga cgcctatcag gacgcgatgc gttactggca aaataccctg gcggggacgt
    18181 tgccgattct tgatatcccc accgatcaac cgcgtcagaa ggtggcacgt taccaaggcg
    18241 cgtttgttgc cttcgcattg tctgccaaca catgcgagcg agtgttggcg gcggcgcgtg
    18301 cgcagcgcgt gtcgttgtat aactatctgc tgaccgcctt cgtcctcctg ctgcatcgta
    18361 atgcgcgtca acaggaatac atcgtcggta tgccgattgc tgcgcggctg acgaaagaac
    18421 aggagcatat gatcgcgccg ctggtcaacg tactaccgct gcgcttacct cttgacgaag
    18481 cggcatcgtt ttccgagtta gtacagacga tcagaggtat tctgtttgcc gctttcaggc
    18541 accagcgtct cgaatttacc gacatagtgc gcgcagtgaa tgtggatcgc agcgccggac
    18601 atttcccaat atatcagtgc atgttccaac tcgacaatat gcctctggcg agcccgacac
    18661 taaacggtgt caacgttaca ccattattgt tggataccag tgcatctcag gtggatattt
    18721 cgctaagtat gcagcatatc gatgggcgca tcaccggcac tttcgaatac gacgctgggt
    18781 tatacagtgc ggatcgtatt cagcacctgg tggcgcagtg gagagaactg ctagatgagg
    18841 ccagcagcca acccacgcag ttagtccgcg atttgattcg tttcacccct cgagagcacg
    18901 cttggctggc gcggcacaac gccactgaag ttgctctgcc gcccgtagat aacctgctgg
    18961 cgctagtgtt accgcactgc cagcagcgcc ctacacaggt ggctttgcgc cacgctgacg
    19021 acgccatgac ctatggcgaa ttgcaacagg ccacgatgca gatgtgtact tggctgcgtg
    19081 cgcaaggcgt aaaacgcgga gaatctgtcg cactgcaact gcctttttgt ttcgaattga
    19141 ttatcgcaca attggcgatc ctctctttgg gggcgagcta tgtgccgctg gatggtaacg
    19201 cgccagcggc ccgcaatgcc ctgatcttgg cgcaggcaac gccgtgcatg ctgctggtgg
    19261 cgcaaccgct ggaatcccct catgggctga cgataccttg ggtgctagtg cctgactggc
    19321 gtagcctgct gacagaaata ccgaacctgc cagttagtgt tgcgccagat gctttagatt
    19381 gcgatgcggt ggtgatcttt acctccggta ctaccgggca gcccaaaggc gtccgactca
    19441 gccaacgtaa tctggtcaac ttaaccgcat cctttatatc cagctatcaa gtgacgcatc
    19501 aggatgttct gctacccatt acctcagtcg cgtctgctag ctttgtcggg gaggtactgc
    19561 cgctgctcgc cgctggcggt accttagtat tggcgcaaaa agcgcagagt ttggatagtg
    19621 atgcgctcat tgcgctgctg gcatctcagc gggtgactat cctgagcacc accccatcgc
    19681 tttctgccag cctctctgtg ctggctcagt cgatgggatc gctgcgcttg tttctgtgcg
    19741 gtggtgaagc gttagagtat gagcagatag cgccgcttct gccgcacatg gcagtggtta
    19801 acggttatgg cctgaccgaa agcggtatct gctcgaccta ctttcctgtc gcaaagcgta
    19861 gggagcaaga aacgggagca ttgcccattg gccgcccgat tcagaacacc caagcttatg
    19921 tggtggatgc atataatcgt ttggtaccgc caggtgcctg tggagaactc tgtttctctg
    19981 gtctgggcat ttcgcccggc taccttgatg cacgacagga tcccgagcgc tttgtcgagt
    20041 tgccggaata ccctggcgtt cgggtgctga aaactggcga tcgtgctcgt tgggcaaccg
    20101 acgggatgtt gttctatctc ggcagacaag atcgccaagt gcagatccgt ggataccgag
    20161 ttgaactggg cgacatcgaa agcctactga aacagcatcc ggatattgct gatgcgtggg
    20221 tcgatgtgcg acgcaatgcg gcggcaacgc cgctactagt ggccttctat tgcagcgtca
    20281 acggcgttgc gctggatgct cagcaattgc gcgtatggct cagcctgcgg cttccgttgc
    20341 atatgctgcc gttgctctac gtgccgctga gcgccatgcc gctaggtgta aacggcaaaa
    20401 tcgatcccca gtgcctgccg ctggtcgatc tgcggcagtt ggaagggccg ggcgagtatg
    20461 tccctccggc aaccgaactg gaacagcgtc tggcggagat ttggcagcag ttgcttggcc
    20521 tggagcgtgt cggcaccacc acaaatttct tcgatctcgg tggacactca cttctgctag
    20581 tacagatgca gcaatacatc gggcagcagt gcggtcagca cgtggcgttg gttgacctgc
    20641 tgcgcttcac taccatcaaa cgcttggcgg aatttctgct ggccccggac gcagcgcaag
    20701 ggaccacagg agatcagaca caactgcgtg cggcgaagca gcgtttggcc tttggtcaca
    20761 cgcgctgggc agccacaacc gacagtcatc actgattcca ctgaatgaaa cgcgtgataa
    20821 acctgaatat agggggaaag caacatggca gagaatgatt ttggtatagc tatcattggg
    20881 atggcggggc gtttccctca agccgatacg gtacaggcgt tttgggaaaa tcttctggca
    20941 agccgagagt gcatttcctt ttacagcgat gaggaactgc tggcgatggg aatcagtcct
    21001 gaatttgttc agcacccaga ttacgtcaag gccaaagggg aggtagctga catcgataaa
    21061 tttgatgctg ctttcttcgg tatcgcaccc cgagaggcgg agctgatgga tcctcagcac
    21121 cgtgtcctgt tggaaaccgc gtgggcagcc ttcgaagatg cgggctatgt ggctgccgat
    21181 tatccggggg atgtgggtat ttttgctggc aaaagcatgg attcctattt gatgctgaac
    21241 ttgatgccgc actttaaacg cgttttctct tctggcagtt tgcaggcggc cattggtaac
    21301 gacaaagaca gtatcactac taccatcgcc tatcacctca acctgcgcgg cccggcgatc
    21361 acggtgcaga cttcctcatc gacatccttg gtggcggtgt gcgtggcatg ccaaagtctg
    21421 ttgacttggc agtgtgacat ggcgattgct ggcggagtaa cgctggggcc accagcaaaa
    21481 accggctact tgtcgcagga aggtgggatc accgccgccg atggccactg tcgggcgttt
    21541 tccgataaca gcagcggatt tgtgcccggc accggcgcgg ggctggtggt gttaaagcgt
    21601 gttgatgaag cgctgcgtga tggcgataac atctatgcgg tcatcaaagg atttgccgtc
    21661 aacaacgacg gttcggagaa gatcagttat accgcgccga gcgtggatgc ccaagcgcgc
    21721 gccattgctc aggcacagcg gttggcaggt ttgacaccgc aggatatcac ttatgtggaa
    21781 gcccacggta ccggtacgcg tttgggcgat ccggttgagt tttctgcgct aagccaagcc
    21841 tttgcgggcg cgtcacaaaa gcagtactgt gcgctggggt cggtaaaaac caatatcggc
    21901 catttggata cggcagcggg cgtcgcaggg ttgataaaaa ccgcgctggc agtgcagcag
    21961 ggaataattc ccgcaacatt gcactttgaa cggcccaatg cacagatcga tctgaccaat
    22021 agtccgtttt acatcaacac tacctgtcaa ccgtggcaac cggaaagcgg catccgtcgc
    22081 gcgggcgtca cctcgcttgg catgggcggc accaatgccc atgttgtgct ggaacaagcg
    22141 ccagccgttg acttgcaagc gcgagcgcct gtacctgcct acagtatttt gccgttttct
    22201 gccaagaccg acagtgcctt atcctcgggg ctggcgcgtt ttgccgattt tctgcaacat
    22261 gaatcgctgc cggataggcg ggatctggcg tggacactct cacagggacg taaagccttc
    22321 gcacatcgcg ccgcgctcgt aaccagagat ctacatgctg ccgggacgct gttgcagcag
    22381 gccgcgacag cgccgtttgc tcgtggtgta gcgcagacac agctcgggct ggggctgttg
    22441 ttttccgggc agggtagcca atatcagcgc atgggccatc agctctatca ggtttggccg
    22501 gcctacgccg atgccttcga ccgttgcgcg acgttactcg agcgtgaata ccaactggat
    22561 atccgccatg agttgttcag agcagaagtc tcgttagccc aaggcgaacg ccttgcgcaa
    22621 acctgcctca cacaaccgct gctgttcagc gtcgagtatg ccttagccca attgtggctc
    22681 agttggggaa tcacgccaac ggtaatgatt ggtcattcgc tgggcgaatg ggtggcagcg
    22741 acgttggcag gcgtgttctc tttagaggat gcgttgcgct tggtggcgcg tcgagcagag
    22801 ctgatgcatc aggcaccaag cggtgccatg ttgatggtgg cgctgcccga agcacagatt
    22861 cgcgcactga ttaccgcccc gctggcgatt gccgctgtta acgcccctga ctattcggtt
    22921 atcgccgggc ctacgtcgga gatcctcgcc gtcagccagc gtctgacgga gcagaacatc
    22981 atcaacaaac gattgcatac ctctcatgcc ttccactcta gcatgatgca agatgcggcg
    23041 caggcgctac gtcaggcatt tgagaatgta cgactgaacc cgccgacgct caccatcatc
    23101 tctaccgtaa ccggcgcgca cgtcagtgcc gacactctca ccacaccgga ctactggatt
    23161 gaacagatgc tgatgcctgt gcagttctcc gctgcactgc aagaggcgca agctacattt
    23221 gatgtcgatt ttctggaaat cgggccaggt gccaccctga cccagctgac caacggtcat
    23281 gcattaggtg atcgtctcgc gttcagctcg ctgccagcag gtgcccgcag cagcgatgag
    23341 cacaaacata tcttagatac cgtggcagcg ctttgggtgc gagggcataa catcgatttg
    23401 tctgcgtttg caggtgagca accgcgccgt gtctctctac cgacctacgc ctttgacaaa
    23461 atccgttact gggtcgacag tccagaagaa cagaggagcg ccgtaacgcc ggtggcggac
    23521 gcgggaagtg tcatccctag cgaaccgtcg gtgcgccgtc agccgcgtcc ggcgttttcg
    23581 gtgccttacg cggctccaga gagcaaaacg cagcgcggct tagtggcgat ttgtgaagca
    23641 ttgctgggaa ttgacggctt gggcattgac gacaacttct tcgaagctgg cggacattca
    23701 ttgatgctgg gcatgttgct ggcgcaggta caggaacggt tcgccgtcac tctttccttt
    23761 ttcgacgtga tggaggatgc cagcgtgcgc gcgttagcgc agttggtcga gcaggagcag
    23821 caggatgatg gagggtccgc acttgccgtg ctagttaacg acatgattaa tgagtgacgt
    23881 tggcaaaagt atttaacaca gcgaaaagga aaggacggtc atgacgatac atcatgccgc
    23941 attggcgcga atgttaccgg cggaaaaaaa ggaaaagcta ttacgacagt tggcgcaatc
    24001 aggcgtgtcg cccagccgca ttcccatcat caaagcggat ccggcgcagg caatcccgct
    24061 gtcgtttaat caggagcggt tgtggttttt gcaaaaatac gacagtaccg ccactaacta
    24121 caacctttat gtggtgtatc gtttgcatgg cgtggtggat atgccgatgc tcacggaggc
    24181 attgcgccat gtgcaagcac gccacgccat cttgcgtacc cgtatcatag tacgtaacga
    24241 tcggccttgc caggtgatag atgatgcgtc ttcgttagtt ctcgatacgg tcacgctggc
    24301 agcgcaggcc ccaacttcag cgctagatgc ggtaattcag caggtgataa acacccggtt
    24361 cgatctggca cgcggtcctc tgtggggggt aactcagatt attcaacccg atcaaggttg
    24421 tcatttggtg ttttgcgcgc accatatcat catcgacggt atctcgttac ggctattgtt
    24481 cgatgagtta cagcagcagt atgctcgatt gcatgctggt aatgaaacgt cgctgcctcc
    24541 gccgccgctg caatatgccg attatgcctt ctggcagcgc gaatggttcc aggatacctt
    24601 gctggcgaac gaacttgcct actggcgtgc gcgtttgcaa gacgcgcctc tgctgtcgac
    24661 atttccatcg ctgcacccgc gcccggcaca accctctaca catggttccc ggttcagcat
    24721 caccctggat gaaacattaa gcctcgcgtt gaaacacgta gcccgcacgc aggaaacaac
    24781 gccgttcgtg ctgatgctga ctgccttcca actggtgctg atgcgttacg ctcagcaaca
    24841 gcgattggtg ataggcatgc cagtatcggg gcgcattcgc ccggaattgc agagtagcat
    24901 tggttattat gccagcactg cggttatcta caccgatttt aatggggttg aggtagggcg
    24961 cgaggcgctc cagcgtgtga aggccagcgt gaaagagacg caggggcgcc agcaactgcc
    25021 gtttgaaaac ttggtgaata tgctggatct ccctcgcagc ctttcccatt caccgttgtt
    25081 ccagatcctc tatatctacc ataaccacgt gaccccacgc gcttttacct tggctggtgc
    25141 gtattgggag caggtgacgt atcacaatca gaccgtcaaa tacgacatga cagtcgaggt
    25201 gttccaaaac gacgccacgt tcgacgtctc ctttgagtac gatttggggt tatacgatgc
    25261 tgatgtggtg aagcagattg ccgaggcgct gcgtcagcac tgcttatcgt tgacatcatc
    25321 actggagacc ccgatagggg cgatccctct gcatgcaccg gagaccgcaa cgccgcggcg
    25381 tgatccgctc aacgccacta acgtcccgtg gctcgggccg caggatgtgc tgcgtatcat
    25441 tgaacagcgc tgtgtgcagc acccaaagca actggcaata cagcagcatg acggcacact
    25501 gacctacgct gagctctggg cgcgtgtgca gttcatcgcg atgcgttttc gagcgcatgg
    25561 catacaaccg ggcgatcgta ttggcgtgct gttgccacgt cacagggatg tgattgcaac
    25621 catgttggcg acgtggtttg tgggggcgtg ctacgtgccg ttcgatattc atcagcctgc
    25681 cgcgcgtttg caacgcctta tgcaacgcgc gcgtttggtc tgtctggtgg tccgtcagcc
    25741 gggagagtgg ggtgaaattg tacaactgtc gttgccggaa ttgatgcagg acatgtcgaa
    25801 tgcgatccgg tattctacac cttgcgcgct gttgccggat atgcaggcct acctgttgtt
    25861 tacctccggc agtaccggtg aacctaaagg cgtgtgcgtt gttcaccgcg ggttgctgaa
    25921 cttgttgttg gatatgcagc gtacctttgc ggttggctcg caagaccggc tgctctcggt
    25981 gacgacgcca acatttgata tctcattctt ggagtatctg ttgccgctga tctccggtgc
    26041 gagtctctat ctgacagagg cggaacgcgc cgcagacagc ttccgtatga ttccgctgat
    26101 tgccgactat cgaccaacgc tgatgcaggc gacgccctcg ttctggcatg ggctgttgat
    26161 ggcgggttgg cgtggcgacc cggaactatg tgtgctggca ggcggtgaag cgctgccaac
    26221 gaaagtggcg gaagaactgt tgcgctgttg tggttcattg tggaacctct acggtcccac
    26281 cgaaacaacc atttggtcgc tgaaatcgca gataacccaa gcggaaaaca tcaccctcgg
    26341 cgctcccatt gccaataccc gtatatacat tctggacaat gagggccatc cagtgccgca
    26401 aggcgttgac ggcgagcttt acatcgccgg ggatggtgtg gcgcaagggt atgatgggca
    26461 gcctgagttg aacgcacagt tcttcttgtc agaaccaggg gtccccggtg gcaggatgtt
    26521 ccgcactggc gatctggtca ggagtgatgc gcaggggcag ttgttttttg ttgggcgcaa
    26581 agatagccag atcaaactgc gcggttatcg tattgagtta ggcgaaattg aacggacgtt
    26641 ggcacggcat ccgcatgtgg acgccgcggt ggtggcctgt attgaaagag caccgttaca
    26701 caaagcactg gcagcattca tcatcaccag tgagcctccc tcgctattcg agcaactgaa
    26761 aaacgagctt cggcagcaac tgccagacta tatggtgcca acgctgtggc agagggtggc
    26821 cgacttcccg aacactgaca acggtaaaat cgatcgtaag cggttagcgg aaaatttcgt
    26881 tgccgatagc tcccttgtgt cgccgcagac gcaagcgctg agcgacacgg aacagatgtt
    26941 gctggcgctg tggatgcgct atttgccgat aaaaaacgtc gatcctgagt gcgatttctt
    27001 tcgcttgggg ggacattcgc tgttggcggt aacgttggta gcagagatca accgcacttt
    27061 tcactgcgcc ttgaccctga aggacatttt ccactactcc acactacggg cactgagtgc
    27121 gcgtattgca cagcaatcta tcacggacgc tgccgcgtct caggatgact gggtgatagt
    27181 gcacgaccct gagcatcgtc atcaaccgtt cccattgacg gatgttcagc gcgcctactg
    27241 gcttggacga cagacgggtg ctacctcgat cgcgacccac atctaccatg aatttgacgt
    27301 agaacacttt aatgttacgc gttttaccca tgcggtgaat gcgctgatcg ctcgccatga
    27361 aatgctacgt gcgcgggtac tccccgacgg tactcagcag attctggcgc aagtgccggc
    27421 gtatcagtta gagcagcgcg atctgagtgc tttgtcccct aacgcacgaa acgatgcctt
    27481 gatggcgatc cgcgatcggc tgtcgcatca tgtgcatccc gcagatcgtt ggccgctgtt
    27541 tgatttcagt tattcggctt gcacggcgca acatggccgc ttgcatttca gtctcgatct
    27601 gctgattgcc gatgctctga gtatgcgcac gctacagcag gagttgatga tgctgtaccg
    27661 tgagccccat gtgtcactgc cgttgctacc gttctctttt cgtgactacg tgcaggcgct
    27721 gttggtagag caggcgagtg aagcctatgc acgcgatcag gcctattggc aacgggcgct
    27781 gccgcagctg tatggcccac caacgctgcc cgtacagggc gatttggcgc aactgtctgc
    27841 gatcaggttc gtacgtcgcc gtcatcggct gtcagcccac aactggggag tgctgagcgc
    27901 gctggcccaa cgcacacgta tcaccaagac ggcattgttg ttgacagtct ttagccaagt
    27961 gctggcacgt tggagcctta gcccgacgtt tacgctcaat ctgacgttgt tcaaccgccc
    28021 gcagggttac cccaacgcag aggcagttat tggtgatttt accgctgtca gcttgctgaa
    28081 tgtttgttac gacagccagc actcttatgc ccacaacgct cagcgtattc aggtgcaact
    28141 gtgggaagat ctcgaacatc gtcgtttcag tgggatccgc gccagcgagg cgctgatcca
    28201 tagcggtcgt ttccatgcgc cgatgccggt ggtattcact agtatgttgg atatcgacgg
    28261 ggagacgact gcgcaagacc ctcgggacac aacccgtttt actctgtgtc cggacgccaa
    28321 tattacccaa acaccgcagg tgtggctcga tcaccaggtg atcgagttgg ctggggagtt
    28381 gcatttcaac tgggacgcgg tcgagcaact gtttgatacc acgctgctgg atcagatgtt
    28441 tggtgcttat tgtcatgcgc tgcaggcgct ggttgccatg ccgcaaagtt ggtggggggt
    28501 aaatagttct ctggcgctgc ccaccgttag tgcaccggtc acgcaggctc ctgcacctac
    28561 ggccttgttg caccatgg
//
LOCUS       DAZFRE010000071        27734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFRE010000071 DAZFRE010000000
VERSION     DAZFRE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.071"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            90..1238
                     /gene="mtlD"
                     /locus_tag="ACHKUI_003293"
     CDS             90..1238
                     /gene="mtlD"
                     /locus_tag="ACHKUI_003293"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6599440.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAEISGLDANSEVVSEAVTAYKAMQ"
     gene            1238..1825
                     /gene="mtlR"
                     /locus_tag="ACHKUI_003294"
     CDS             1238..1825
                     /gene="mtlR"
                     /locus_tag="ACHKUI_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6599441.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1836..2045)
                     /gene="yibT"
                     /locus_tag="ACHKUI_003295"
     CDS             complement(1836..2045)
                     /gene="yibT"
                     /locus_tag="ACHKUI_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6599442.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            2329..2691
                     /locus_tag="ACHKUI_003296"
     CDS             2329..2691
                     /locus_tag="ACHKUI_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6599443.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            3250..3933
                     /locus_tag="ACHKUI_003297"
     CDS             3250..3933
                     /locus_tag="ACHKUI_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6599444.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPA
                     ESPQRKNKK"
     gene            3977..8572
                     /locus_tag="ACHKUI_003298"
     CDS             3977..8572
                     /locus_tag="ACHKUI_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6599445.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVVGG
                     LLSSGVQASTALDGGGASESTPLTDTWIAIGQGAKASSVDTSPSGGPGTSSIAIGQEA
                     QSGIGSTSLGFKSQATGERSVALGQTADATGNRAIAIGSGAKSTADYSLTLGNGSKAF
                     AENATAIGKDARAEGKGSQAFGKVALATGENAIALGSNSNASGDNAVALGSTSQAYGD
                     SSLAIGELSETNAENAIALGKLSNASKINSIALGSNSTASGVGSVALGENSFAGGINS
                     LALGSQSNANGDNAVALGVGSVAEQDNTVSVGNSTTQRKITNMAAGLILPGSTEAING
                     SQLHALSASVAARLGAGAGVNGDGSINAPSYKLKSGTYNNVGEALLGIDNDTLHWDKT
                     EGAFSASYIVKNADGSPKSSIAQNKIINVAKGTISATSTDVVNGSQLYDLQQDALLWN
                     GTAFSAAHGTEATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIATNTTDI
                     ATNTANIADNTANIATNTTDIATNTADIATNASSITTLNADVDTLEKDALLWNGTAFS
                     AKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNLTDSVGDLKD
                     DSLLWNKTAGAFSAAHGTEATSKITNLLAGKVSSDSTDAINGSQLYGVADSFTSYLGG
                     GADISDAGVLTGPTYTIGGTDYTNVGDALAAINTSFSSSLGDALLWDATAGKFSAKHG
                     INNDPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNADGSITTPTYA
                     IAGGSYDNVGDALEAIDTTLDDALLWDETANGGNGAFSAAHGKDKTASVITNVANGAV
                     SATSSDAINGSQLYSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEALDAL
                     DNNALLWDATANNGAGAYSASHDGKASIITNVADGNIGEDSTDAINGSQLNATNMMIQ
                     QNSEIINQLAGDTSKNYIGDNGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNTVAKG
                     DSSVAIGHGSNSIVETSIALGSESVSSRLIVKGSRNTRVTENGVEIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVG
                     EDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTR
                     GAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            8984..10639
                     /gene="lldP"
                     /locus_tag="ACHKUI_003299"
     CDS             8984..10639
                     /gene="lldP"
                     /locus_tag="ACHKUI_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6599446.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            10639..11415
                     /gene="lldR"
                     /locus_tag="ACHKUI_003300"
     CDS             10639..11415
                     /gene="lldR"
                     /locus_tag="ACHKUI_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6599447.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            11412..12602
                     /gene="lldD"
                     /locus_tag="ACHKUI_003301"
     CDS             11412..12602
                     /gene="lldD"
                     /locus_tag="ACHKUI_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6599448.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            12650..13123
                     /gene="trmL"
                     /locus_tag="ACHKUI_003302"
     CDS             12650..13123
                     /gene="trmL"
                     /locus_tag="ACHKUI_003302"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6599449.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(13178..13999)
                     /gene="cysE"
                     /locus_tag="ACHKUI_003303"
     CDS             complement(13178..13999)
                     /gene="cysE"
                     /locus_tag="ACHKUI_003303"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6599450.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRAQAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(14079..15098)
                     /gene="gpsA"
                     /locus_tag="ACHKUI_003304"
     CDS             complement(14079..15098)
                     /gene="gpsA"
                     /locus_tag="ACHKUI_003304"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6599451.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(15098..15565)
                     /gene="secB"
                     /locus_tag="ACHKUI_003305"
     CDS             complement(15098..15565)
                     /gene="secB"
                     /locus_tag="ACHKUI_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6599452.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(15628..15879)
                     /gene="grxC"
                     /locus_tag="ACHKUI_003306"
     CDS             complement(15628..15879)
                     /gene="grxC"
                     /locus_tag="ACHKUI_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6599453.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(16020..16451)
                     /locus_tag="ACHKUI_003307"
     CDS             complement(16020..16451)
                     /locus_tag="ACHKUI_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6599454.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            16696..18240
                     /gene="gpmM"
                     /locus_tag="ACHKUI_003308"
                     /gene_synonym="pgmI"
     CDS             16696..18240
                     /gene="gpmM"
                     /locus_tag="ACHKUI_003308"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6599455.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            18274..19533
                     /gene="envC"
                     /locus_tag="ACHKUI_003309"
     CDS             18274..19533
                     /gene="envC"
                     /locus_tag="ACHKUI_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6599456.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            19537..20496
                     /locus_tag="ACHKUI_003310"
     CDS             19537..20496
                     /locus_tag="ACHKUI_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6599457.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(20483..21517)
                     /gene="waaH"
                     /locus_tag="ACHKUI_003311"
     CDS             complement(20483..21517)
                     /gene="waaH"
                     /locus_tag="ACHKUI_003311"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO6599458.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(21756..22781)
                     /gene="tdh"
                     /locus_tag="ACHKUI_003312"
     CDS             complement(21756..22781)
                     /gene="tdh"
                     /locus_tag="ACHKUI_003312"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6599459.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(22791..23987)
                     /gene="kbl"
                     /locus_tag="ACHKUI_003313"
     CDS             complement(22791..23987)
                     /gene="kbl"
                     /locus_tag="ACHKUI_003313"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6599460.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            24201..25133
                     /gene="rfaD"
                     /locus_tag="ACHKUI_003314"
     CDS             24201..25133
                     /gene="rfaD"
                     /locus_tag="ACHKUI_003314"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6599461.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKSRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25143..26189
                     /gene="rfaF"
                     /locus_tag="ACHKUI_003315"
     CDS             25143..26189
                     /gene="rfaF"
                     /locus_tag="ACHKUI_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6599462.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHILREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHAAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            26193..27173
                     /gene="rfaC"
                     /locus_tag="ACHKUI_003316"
     CDS             26193..27173
                     /gene="rfaC"
                     /locus_tag="ACHKUI_003316"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6599463.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
     gene            complement(27243..>27734)
                     /locus_tag="ACHKUI_003317"
     CDS             complement(27243..>27734)
                     /locus_tag="ACHKUI_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO6599464.1"
                     /translation="KDTLLTRMNDLNRDLVNYSHDNTRTSVGARLAMYEVGLKTYSPI
                     GQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVALTILAFSAI
                     FIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKVQSDQCL
                     LDK"
BASE COUNT         7107 a         7111 c         7069 g         6447 t
ORIGIN      
        1 aaagcccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
       61 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
      121 tcggtcgtgg ctttatcggt aaactgctgg cggacgcggg tatccaactg acgtttgccg
      181 atgtaaatca ggtggtgctt gatgccctga atgcccgtca tagctatcag gttcatgtgg
      241 ttggtgaaac cgagcaggta gataccgttt ctggcgtcga tgccgtcagc agcattggcg
      301 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggccctgtcg
      361 tgctggaacg tattgctccg gccatcgcca aagggctggt gaaacgtaaa gaacaaggta
      421 atgaatcccc actgaacatc atcgcctgtg aaaacatggt ccgcggcacc acgcagctga
      481 aaggccatgt gatgaacgcc ctgccggaag atgccaaagc gtgggtagaa gaacacgttg
      541 gctttgttga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
      601 cgctggaagt gacggtagaa accttcagcg aatggattgt cgataaaacg cagttcaaag
      661 gcacgctgcc aaacatccct ggcatggaat taaccgacaa tctgatggca tttgtcgaac
      721 gtaaactctt caccctgaat acaggtcatg ctataaccgc gtacctcgga aaactggccg
      781 gtcatcagac cattcgtgac gcaattctcg acgagaaaat ccgcgcggtg gtaaaagggg
      841 caatggaaga aagcggcgcg gtactgatca agcgctacgg ctttgatgct gacaaacatg
      901 cggcatacat ccagaaaatc ctcggtcgtt tcgagaaccc gtacctgaaa gatgatgtag
      961 aacgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
     1021 tgctcggaac cctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg
     1081 caatgcactt ccgcagtgaa gatgacccgc aggctcagga actggcagcg ctgattgccg
     1141 acaaaggccc acaagccgcg ctggcagaga tttccggtct tgatgccaac agcgaggttg
     1201 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggatac
     1261 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     1321 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
     1381 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     1441 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgatggtc cgctgggcga
     1501 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
     1561 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
     1621 ttttaccgac gacgaaatac ttggaccctt tggtgaactg cattgcgtgg cggcgttacc
     1681 accgccgcca cagtttgaac ctgcagactc cagtttgtat gcaatgcaaa ttcagcgtta
     1741 ccaacaggct gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag
     1801 cttaaaaaaa gcctttcaaa agtaagtact ttcgcttact tctcctctac ctgtttcgga
     1861 cgataaagct gacgataata ctccagacgt tgtagatata acggcacgct ttcatcaggt
     1921 attccggacg gaatcgcgtt acggggaggt agttttttca gatactcccg gaacgcctgg
     1981 ctggatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
     2041 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt
     2101 agatcccgat ataaatgaca ttaaggaaaa cgtgctcaat cctcaataat cacctgcttc
     2161 tgttgactat cttttttctt taaatttctt aactaatcaa tgtattgggt gattttttaa
     2221 cgcattaatg gttacaatct ggaactatta caatgccatc agttgtgaac aaagcacctg
     2281 gtcgcgcata ctagaggcta taaatttatc tctatcagaa gtcatcacat gaaagaagtc
     2341 gaaaaaaacg aaatcaaacg tctcagcgat cgtctggacg ccatccgtca ccagcaggcc
     2401 gatctatcgc tggttgaagc cgcagacaaa tatgccgaac tggaaaaaga gaaagccacg
     2461 ctggaagcag aaattgctcg cctgcgcgaa gttcacagcc agaaactcag caaagaagcg
     2521 cagaaactga tgaagatgcc gttccagcgt gcaatcacca aaaaagagca ggctgatatg
     2581 ggcaagctga aaaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggc
     2641 cgcgaaatgg gcctgcaaga gatgacaggg ttctcaaaga ctgcgtttta agttcctttc
     2701 tcgtcgctgt aatcacagcc tctcaccatt tactcgctcc tgctggcaca tgcaggagcc
     2761 tgtttcagag atcgaatctt tataacgttc ttaacattat atattcaact ccacctacac
     2821 ttctgagcga tcgtagcagg ctaaattctg aaaatcaggt acacattaaa gaatgatgta
     2881 tcaatatcaa caatgaattt ccgcatatca atgctcgaaa cataattaat tatttaaatt
     2941 tttcaatcag cttcgcaaat tccacaataa aacatcactc aactgaatat ttacctttcc
     3001 ctgccctgtg cctttatccg gtgaaacatt tgacatcgat tgacaccgat cattaaaatc
     3061 cgcgcgatta atattattgg ctacaaactg aaatcgtttg acatttaata acatattcgc
     3121 acaaccaatt aatacaaaaa ctctctaaca aatttatttt gcggatatgc ctcattccaa
     3181 tcattcataa aaaaaatgat tgtgccgtaa ttatcaactg tatcgggatt atgaaaagga
     3241 attcatccta tgaacctaaa gaaaacactg ttaagcgtgt taatgatatt gcaactttgc
     3301 ttattggtag ggtgtgacta tattgaaaaa gcgagtaagg tcgacgatct cgttacacag
     3361 caagagttac aaaaaagcaa aattgaggcg cttgaaaaac aacaagaact cgacaagcgc
     3421 aagatagaac actttgaaaa acaacaaact accatcataa acagtaccaa aacgctcgct
     3481 ggcgtggtga aggcagttaa agacaaacag gacgaatttg tctttacaga atttaacccg
     3541 gcacaaaccc aatacttcat tttaaataac ggttctgttg gtttggcagg gaaaatactg
     3601 tctattgacg cagtagaaaa cggcagcgtt attcgtattt cactggttaa cttattaagt
     3661 gttcctgtat caaatatggg tttttacgcg acatgggggg gagaaaaacc caccgacatc
     3721 aacgcattag caaaatggca gcaattgcta tttagtacct caatgaactc ctccctgaaa
     3781 ttattaccag gtcaatggca agacattaat ttgacgctaa aaggtgtctc gcccaacaac
     3841 ctcaaatatc tgaaattagc catcaacatg gcaaatattc agttcgaccg tcttcaacct
     3901 gctgaatctc cacagcggaa aaacaaaaaa taaatgcagc ttctgcgctt atatcaagga
     3961 atagagagca tcaataatga acaaaatatt taaagttatc tggaatccgg caacaggtag
     4021 ttacaccgtt gccagtgaaa cagcaaaaag tcgtggtaaa aaaagcgggc gcagtaagct
     4081 gttaatttct gcgttggttg tgggtgggtt gttgtcgtct ggagtacaag catcgaccgc
     4141 gcttgatggt ggtggggcta gcgaaagcac tccattgacc gatacctgga ttgccattgg
     4201 tcagggtgct aaagcatcat cagtagacac ttcaccatca ggaggaccag gaacatcaag
     4261 tattgcaata gggcaagaag cccaatctgg gataggtagt acctcattag gtttcaagag
     4321 ccaggctaca ggagaaagat ctgttgcttt aggacaaaca gctgacgcaa ccggtaatag
     4381 ggccatagca atcggtagtg gagctaaatc gactgccgat tattccctta cgctggggaa
     4441 tggatctaag gctttcgctg aaaacgccac ggcgataggc aaggacgcaa gagcggaggg
     4501 caaaggtagt caggccttcg gaaaagtggc cttggccact ggggagaatg ccattgcctt
     4561 gggtagtaac agcaatgcat ctggtgataa cgccgttgcg ctgggtagta caagtcaggc
     4621 atatggcgac tccagccttg caataggtga gttaagtgag accaatgctg aaaatgctat
     4681 tgcactggga aaactcagta acgcaagtaa aatcaattca atcgccctgg gtagtaacag
     4741 cactgcatct ggtgtgggct ccgttgcgct gggagagaat agttttgctg gaggcataaa
     4801 cagtcttgct ttgggtagtc aatccaatgc aaacggtgat aacgcagttg ccctcggtgt
     4861 aggatctgtc gcagagcaag acaataccgt ttcggtaggg aacagcacaa cacaacgcaa
     4921 aattaccaat atggctgccg gtcttatact accaggcagt actgaggcca ttaacggttc
     4981 acaacttcat gcattaagtg cctcagtcgc agcccgactc ggggctggtg ctggcgtaaa
     5041 tggagatgga agtataaatg cgccaagcta caagctgaaa agcggcacct acaataatgt
     5101 gggagaggcg cttttgggaa tcgacaatga cactctgcac tgggacaaaa ccgagggagc
     5161 atttagtgcc agctatatcg tcaaaaacgc tgatggcagc ccaaagagca gcatagccca
     5221 aaacaaaatc attaacgttg caaagggtac tatttccgcc accagcaccg atgtagtaaa
     5281 cggctctcaa ttgtacgacc tgcagcagga tgcgctgttg tggaacggca cagcattcag
     5341 tgccgcacac ggcaccgaag ccaccagcaa aatcactaat gttgaagatg gtactatttc
     5401 agacaccagt aaagatgcgg ttaacggttc ccagttgaaa gaaaccaaag acgatgtggc
     5461 caccaacaca gccaatatcg ccactaacac cacggatatt gcaaccaaca ctgccaatat
     5521 cgccgataac actgccaata tcgccactaa cactacggat attgcgacta ataccgccga
     5581 tatagctaca aacgctagca gtatcactac tctgaacgcg gacgttgata ccctggaaaa
     5641 agatgccctg ctctggaatg gcacagcatt cagcgccaaa cacggcaccg aagctaccag
     5701 caagatcacc aatgttaccg ctggcgacct gactgctggc agcactgacg ccgttaatgg
     5761 ctcccagctc aaaaccacca acgataacgt gtcgaccaac accaccaaca tcaccaacct
     5821 gacggattcc gttggcgacc ttaaggacga ttccctgctg tggaacaaaa cggctggcgc
     5881 attcagcgcc gcgcacggca ccgaagccac cagcaagatc accaacttac tggctggcaa
     5941 ggtatcttct gacagcaccg atgccattaa cggatcacaa ctttatggcg tagcggattc
     6001 atttacgtca tatcttggtg ggggtgctga tatcagcgat gcaggcgtat taaccgggcc
     6061 aacctacact attggtggta ctgactacac taacgtcggt gatgctctgg cagctattaa
     6121 cacatcattt agctcatcac tcggcgacgc cctactctgg gatgcaaccg caggtaaatt
     6181 cagcgccaaa cacggcatta ataatgatcc cagtgtaatc actgatgttg caaacggtgc
     6241 agtctcgtcc accagtagcg acgccattaa cggttcgcaa ctttatggcg ttagtgacta
     6301 cattgccgat gctttgggcg ggaacgctgt ggtgaatgca gacggcagta tcactacacc
     6361 gacttatgcc atagcgggcg gcagttacga caatgtcggt gacgcgctgg aagcgatcga
     6421 taccacgctg gatgacgccc tgctgtggga tgagacagcc aatggcggta acggtgcatt
     6481 tagcgccgct cacgggaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc
     6541 agtctctgcc accagtagcg atgccattaa tggctcacag ctctatagca ccaataagta
     6601 catcgctgat gcgctgggtg gtgatgcaga agtcaacgct gacggaacta tcactgcacc
     6661 gacttacacc attgcaaata ccgattacaa caacgtcggt gaagccctgg atgcgcttga
     6721 taataacgct ctgctgtggg atgcaacagc caataacggc gcaggtgctt acagcgccag
     6781 tcatgacggc aaggccagca ttatcacaaa cgttgctgat ggtaacattg gtgaagatag
     6841 caccgacgct atcaacggtt ctcagttgaa tgcaaccaac atgatgatcc agcagaacag
     6901 cgaaatcatc aatcagcttg ccggtgacac cagtaaaaac tacatcggag ataacggtgc
     6961 aggtattaac tatgtacgta ccaacgacag cggcttagcg ttcaacgatg ccagcgcagc
     7021 aggtattggc tctaccgctg tggggtataa cactgttgcc aaaggcgata gcagcgtggc
     7081 aatcggtcat ggcagcaaca gcatcgttga gacgagtatt gcgctgggta gcgaatccgt
     7141 ctccagccgt ttgattgtga aaggctcccg taatacccgc gttacggaaa atggggtcga
     7201 gatcggttat gacaccacgg atggcgaact gcttggcgcg ttgtcgatcg gtgatgatgg
     7261 taaatatcgt caaatcatca acgtcgcgga tggttctgaa gcccatgacg ctgttactgt
     7321 tcgccagttg caaaacgcca ttggtgcagt agcaaccaca ccaaccaaat actatcacgc
     7381 caactcaacg gctgaagact cactggcagt cggtgaagac tcgctggcaa tgggcgcgaa
     7441 aaccatcgtt aatggtaatg cgggtattgg tatcggcctg aacactttag ttctggctga
     7501 tgcgatcaac ggtattgcta tcggttctaa cgcacgcgca aatcatgccg acagcattgc
     7561 aatgggtaat ggttctcaga ctacccgtgg tgcgcagacc aactacactg cctacaacat
     7621 ggatgcaccg cagaactctg tgggtgagtt ctctgtcggc agtgaagacg gtcaacgtca
     7681 gatcaccaac gtcgcagcag gttcggcgga taccgatgcg gttaacgtgg gtcagttgaa
     7741 agtaacggac gcgcaggttt cccagaatac ccagagcatt actaacctga acactcaggt
     7801 cactaatctg gatactcgcg tgaccaatat cgaaaacggc attggcgata tcgtaaccac
     7861 cggtagcact aagtacttca agaccaacac cgatggcgca gacgccaacg cgcagggtaa
     7921 agacagtgtt gcgattggtt ctggttccat tgctgccgct gacaacagcg tcgcactggg
     7981 cacgggttcc gtagcagacg aagaaaatac gatctctgtg ggttcttcta ccaaccagcg
     8041 ccgtatcacc aacgttgctg ccggtgttaa tgccaccgat gcggttaacg tttcgcaact
     8101 gaagtcttct gaagcaggcg gcgttcgcta cgacaccaaa gctgatggct ctatcgacta
     8161 cagcaacatc actctcggtg gtggtaacgg cggtacgact cgcatcagca acgtttctgc
     8221 tggcgtgaat aacaacgacg cggtgaacta tgcgcagttg aagcaaagtg tgcaggaaac
     8281 gaagcaatac accgatcagc gcatggttga gatggataac aaactgtcca aaaccgaaag
     8341 caagctgagt ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttacac
     8401 gccgggtgcc agcatggcgt ctattggtgg cggtacttac aacggtgaat cggcagttgc
     8461 tttaggtgtg tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac
     8521 caatagccag ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat
     8581 taaagaatgg gtaaacgcca cattgcctga tgcgctacgc ttatcaggcc tacggggtga
     8641 ttcagcacat tcctgtaggc cggataaggc gttcacgccg catccggcaa gataaggcgc
     8701 accagatcaa caatctgagg gcaattctct gatgggaatt gcccttttct ttattacctc
     8761 tctcccagaa ttggccctac caattcttcg cttatctgac ctctggttca caattcccca
     8821 attaaaactc acatcaatgt tgccaacaca taacatttag ttaaccattc attgtcatta
     8881 cccctacaca acacaattgg cagtaccact tttacacaac gtgtgacaag gagatgggca
     8941 acagactcat tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca
     9001 aaactacgat cccgccggga atatctggct ttccagcctg atagcatcgc ttcccatcct
     9061 gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac
     9121 ggtggcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct
     9181 ggcctcggtg gtttatggct tcttctacgg gttgtggccc atcgcgtgga tcattattgc
     9241 agcggtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc
     9301 tattctttcg ataacccctg accagcgcct gcaaatgctg atcgtcggtt tctgtttcgg
     9361 cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct
     9421 ggtcggtctg ggctttaaac cactgtacgc cgccgggctg tgcctgattg ttaacaccgc
     9481 gccagtggca tttggtgcga tgggtattcc gattctggtc gccgggcagg taacaggcat
     9541 cgacagcttt gagattggtc agatggtggg gcgtcagctg ccgtttatga ccattatcgt
     9601 gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaaa cctggcctgc
     9661 ggtcgtggtt gcgggcggct catttgccat cgctcaatac ctcagctcta actttattgg
     9721 gccggaactg ccagacatta tctcttcgct ggtatcgctg ctctgtctga cactgttcct
     9781 caaacgctgg caaccagttc gcgtattccg cttcggtgac ttaggggcgt cacaggttga
     9841 tatgacgctg gctcacaccg gttacacggc aggtcaggtg ctgcgcgcct ggacaccgtt
     9901 cctgttcctg accgccaccg taacgctgtg gagtatcccg ccgtttaaag ccctgttcgc
     9961 ttcaggcggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact
    10021 ggttgcccgt atgccgccag tggtcagcga agcgacagcg tatgccgccg tgtttaagtt
    10081 tgactggttc tctgccactg gcaccgccat tctgtttgct gccctgctct cgattgtctg
    10141 gctgaagatg aaaccatctg acgctatcag caccttcggc agcacgctga aagaactggc
    10201 tctgcccatc tactccatcg gtatggtgct ggcattcgcc tttatctcga actattcagg
    10261 actgtcatca acactggcgc tggcactggc gcacaccggt catgcattca ccttcttctc
    10321 gccgttcctc ggctggcttg gggtattcct gaccgggtcg gatacgtcat ctaatgcttt
    10381 gtttgccgct ttgcaggcta ccgccgcaca acaaattggc gtttctgact tgttgctggt
    10441 tgctgccaac accaccggtg gtgtcaccgg caaaatgatc tctccgcaat ctatcgctat
    10501 cgcctgtgcg gcggtaggtc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa
    10561 acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg cctatgtctt
    10621 aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat
    10681 cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgaa
    10741 cgtcaactgg cgatgcaact cggcgtgtcg agaaattcac tgcgcgaggc gctggcgaaa
    10801 ctggtaagcg aaggtgtgct gctcagtcga cgtggcggcg ggacgtttat tcgctggcgt
    10861 catgacacat ggtcggagca aaacatcgtc cagccgctga aaacgctgat ggccgatgat
    10921 ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg
    10981 catgcagcaa tgcgtgccac gcctggcgaa aaagaaaaga ttcagctttg ttttgaagca
    11041 acgctcagcg aagacccgga tctcgcctca caagcggacg ttcgttttca tctggcgatt
    11101 gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg
    11161 caatcctctg tgaagcatag ccgccagcgg atgtacctgg tacccccagt tttttcacag
    11221 ctgaccgaac aacatcaggc ggtcattgac gccatttttg ccggtgatgc tgacggggcg
    11281 cgtaaagcaa tgatggcgca ccttagcttt gttcacacca ccatgaaacg attcgatgaa
    11341 gatcaggctc gccatgcacg cattacccgc ctgcctggtg accataatga gcattcgagg
    11401 gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc
    11461 tgccgccgtt cctgttccac tatatggatg ggggggcata ttctgaatac acgctgcgcc
    11521 gcaacgtgga agatttgtca gaagtggcgc tgcgccagcg tattctgaaa aacatgtctg
    11581 acttaagcct ggaaacgacg ctgtttaatg agaaactgtc gatgccggtg gcgctaggtc
    11641 cggtaggttt gtgtggcatg tatgcgcgac gcggcgaagt tcaggctgcc aaagccgcgg
    11701 atgcgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtgg
    11761 ctccggctat caaacgtccg atgtggttcc agctttatgt gctgcgcgat cgcggcttta
    11821 tgcgtaacgc actggagcga gcaaaagcag cgggttgttc gacgctggta ttcaccgtgg
    11881 atatgccaac accgggagcg cgttatcgtg atgcgcattc tgggatgagc ggcccgaacg
    11941 cggcaatgcg ccgctatttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc
    12001 tgaacggtcg tccgcatgat ttaggtaata tctcggctta cctcggcaaa ccgaccggac
    12061 tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc
    12121 ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg
    12181 aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg
    12241 gccgccagct ggacggtgtg ctgtcttctg cccgtgcgtt gcctgctatt gcagatgcgg
    12301 tgaaaggtga tatcgccatt ctggcggata gcggaatacg taacgggctt gatgtcgtgc
    12361 gtatgatcgc gctcggtgcc gacaccatac tgctgggtcg tgctttcctg tatgcactgg
    12421 caacagcggg ccaggcgggt gtagctaatc tgctaaatct gatcgaaaaa gagatgaaag
    12481 tggcgatgac gctgactggc gcgaaatcga ttagcgaaat tacgcaagat tcgctggtgc
    12541 aggggctggg taaagagttg cctgcggcac tggctccgat ggcgaaaggg aatgcagctt
    12601 aagggttaga cgaatatctg ctatcctgcc cccgcactaa gggggcagta tgctaaacat
    12661 cgtactttac gaaccagaaa ttccgccaaa tactggcaac atcatccgtc tttgcgctaa
    12721 taccggcttt cgtctgcata tcatcgaacc gatgggattt gcctgggacg ataagcgcct
    12781 gcgccgcgcg gggctggact atcacgagtt taccgccgtt acgcgtcatc atgactatcg
    12841 cgcgtttctc gaagcagaaa atccccagcg tctgtttgcc ctcaccacga aaggtacgcc
    12901 tgctcacagc gccgtaagct atcaggatgg cgattatctg atgttcggcc cggaaacacg
    12961 cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc gtattccaat
    13021 ggtgccggac agccgcagca tgaacctgtc caatgcggtg tcggtggtgg tgtatgaagc
    13081 ctggcgccag ctggggtatc cgggagcggt gttgagagat taagatgcgg taggccggat
    13141 aaggcgttca cgccgcatcc ggcacgatca cagaatgtca gatcccatca ccatactcaa
    13201 acgtatggtt aatgccgttg aaatgctgat ccatatccat tgacggttta tcgctatccg
    13261 gcttgccgac aatgcgtgcc ggaacgccag cggcagtggt gtgcggcggc accggttgca
    13321 gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatattg ccgagaattt
    13381 tcgcgcccgc gccaatcatc acgccttcac gaattttcgg gtgacggtca ccaccagatt
    13441 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca atcaccgccg
    13501 tttcaccaac gacgatgcct gtcgcgtggt cgagcatgat accgcgacca atttttgctg
    13561 ccgggtgtat atcaacctgg aacgttacag agacctggtt ttgtagaaaa atagcctgcg
    13621 cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa gcatgaaaac
    13681 ccttcagata tagcaacggg gttgagtatt tatccaccgc cgggtcgcgg gtacgcactg
    13741 cctgaatatc acaggccgca gaagcgatca tttccgggtc ggcggcgtag gcctcttcca
    13801 ccacttcgcg gatagcaatg gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    13861 agctcagtgc actgcccagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    13921 gcattggctc acaatccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    13981 ccagttcttc acacgacatt gcttactcca cacgataaga taatgaccga cccgttctgc
    14041 gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct gcgctcgtcc
    14101 ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    14161 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    14221 acttctttcg tattgcggta gccttccacc acttgaccaa ttttctcctg cgcgctttgt
    14281 acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta
    14341 caggtaagca ccagatcgcc cagccccgcc attcccataa aggtggcagg atcggcaccc
    14401 agcgccgcgc caagacgcga catttcagcc agtccacggg tgatcagcgc cgtacgcgca
    14461 ttcgcgccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc
    14521 gcaccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa acttttgcca
    14581 cagtgcagca attgctggag gtcatcggca aaggtctggt ccgtcgaggc cagcgaaatg
    14641 gccgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    14701 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttctgct
    14761 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    14821 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc
    14881 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    14941 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    15001 aggacaacct cgtggccatt tcttgccagg gtgatggcaa gagcggtgcc gtacgagccg
    15061 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    15121 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    15181 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    15241 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    15301 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    15361 ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct gccagttgag
    15421 tagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    15481 gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc tggaaagtca
    15541 tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    15601 gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    15661 atccaatgca tacaagtcat cacagccacc aatgtgctgt gcgtcaataa aaatctgtgg
    15721 aaccgtggtg cgaccactgc gtttgatcat ctcttcacgc ttagcggcat tgccatcgat
    15781 cggcaactcc tgaaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    15841 cgggcaggtt tctttggtat agatttcaac attggccata aattagctcc tgttttttac
    15901 cctgaaattt catgttgcag ggcggcagca aaatggagtg tccccagaag cttatatcaa
    15961 taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat
    16021 tatttgccgc gcaccaaagg caagttttcg ccagcccagc cagcgacgcc ttctttcagt
    16081 acgaatactt gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg
    16141 ccagaaccat ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg
    16201 ttggctttga tttcgctcgg caacaggttg atagaacctg cgatatggcc tttacggaag
    16261 tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct
    16321 tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc
    16381 gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata
    16441 atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt
    16501 atacctgcgc ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc
    16561 gcgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat
    16621 cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag
    16681 tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc
    16741 tatggctatc gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg
    16801 gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt
    16861 ctgcctgacc gtcagatggg taactccgaa gtgggccacg ttaacctggg tgccggccgc
    16921 atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttcttcgct
    16981 aacccggtac tgactggcgc ggtagataaa gcgaaaaacg caggcaaagc agttcacatt
    17041 atgggtctgc tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta
    17101 gaactggccg ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc
    17161 gacactccgc cgcgcagtgc tgaatcctcg ctgaaaaaat tcgaagagaa atttgccgcg
    17221 ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac
    17281 cgttgggatc gcgttgaaaa agcttatgac ctgctgactc tggcacaagg tgagttccag
    17341 gccgataccg ccgttgccgg tttgcaggct gcttatgctc gcgacgaaaa cgacgagttc
    17401 gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cagcaatgga agacggcgat
    17461 gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg tgctttcgtg
    17521 aacgctgatt tcgatggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg
    17581 accgaatacg ctgccgacat caaaaccgca gtggcttacc cacccgcttc cctggttaac
    17641 accttcggcg aatggatggc gaaaaacgac aaaactcagc tgcgtatttc cgaaaccgaa
    17701 aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa
    17761 gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc
    17821 tccgcagagc tgaccgaaaa actggttgcg gcaatcaaga gcggcaaata cgacaccatt
    17881 atctgtaact atccgaacgg cgacatggta ggtcacaccg gagtgatgga agcggcggtt
    17941 aaagcggttg aagcgctgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg
    18001 ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca
    18061 acgggtcagg cacacacagc acacaccaac ctgccagttc cgctgattta cgttggtgat
    18121 aagaacgtga aagcggttga aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg
    18181 ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa
    18241 tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg
    18301 tttgcaatca ggcccatcat ctacgccagc gttctgagcg ctggcgtatt gttgtgcgcc
    18361 ttttccgccc acgcggatga gcgtgaccaa ctcaaatcca ttcaggctga catcgccgcg
    18421 aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa
    18481 aagcaggaag aagcgatctc tgaagccacc cgtaaactgc gcgaaacgca aaacacgctt
    18541 aatcaattga ataaacagat tgatgagatg aacgcgtcga ttgccaaact agagcagcaa
    18601 aaagccgccc aggagcgcag cctcgccgcg caactggatg ccgcgtttcg tcagggtgaa
    18661 cataccggta ttcagctgat tctcagcggt gaagaaagcc agcgtgggca gcggttacag
    18721 gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgctcagtt gaaacaaacg
    18781 cgtgaagaag tcgctatgca gcgtgccgaa ctggaagaga aacagagcga gcaacaaacg
    18841 cttttatatg agcagcgcgc ccaacaggcg aagctgaccc aggtgttgag cgagcgtaag
    18901 aaaacactgg cagggctgga gtcttccatc cagcaaggtc agcaacagtt gagcgagctg
    18961 cgcgccaacg aatcccgtct gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg
    19021 cgtgctgaac gtgaagcgcg cgaagcccag gcggttcgcg accgccagaa agaagcgacg
    19081 cgcaaaggca ccacctacaa gccgaccgaa agcgaaaaat cgctgatgtc ccgtaccggt
    19141 ggtctgggtg cgccgcgtgg tcaggcattc tggccagttc gcgggccgac gctgcatcgc
    19201 tatggcgagc agctacaggg tgaattacgc tggaaaggaa tggttatcgg tgcctctgaa
    19261 ggtactgaag ttaaagcaat tgccgacggt cgggtgattc tggctgactg gctgcaaggc
    19321 tacggtctgg tggtggtggt tgagcatggt aaaggcgaca tgagtcttta cggctataat
    19381 cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg gccagccgat tgcactggtg
    19441 ggcagcagtg gcggtcaggg cagaccttca ctctatttcg aaattcgccg ccagggtcag
    19501 gcggtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt
    19561 cttgcatttg ccgccctgtt ggcgctctcc tcccccgtaa ttgctggcaa acttgccatc
    19621 gtcattgatg attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc
    19681 gctatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagca
    19741 cataacagcg gtcacgaagt gttgattcat ctaccgatgg caccactgag taaacagccg
    19801 ctggagaaaa atacgctacg cccggagatg agcagcgacg aaattgagcg cattattcgt
    19861 agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca accacatggg cagcaagatg
    19921 acctctaacc tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta caatctttac
    19981 ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggcactggc
    20041 gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc aaaatgaagc ggacatccgt
    20101 gtgcaattca atcgcgcaat tgacctggcg cgtcgcaacg gttcaaccat tgccattgga
    20161 catcctcacc cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac
    20221 attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac gtctacaccg
    20281 ccgaaaaaca ctgtgcccga cacaccgcgt aatccattcc gtggcgtgaa gctgtgcaaa
    20341 ccgaagaagc cgctggaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc
    20401 atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag
    20461 cccgaagcgg caaaacttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt
    20521 gcgaccgcgt tttgtcgcgc cattgccata agcggaaaga ccacagtaac gcctggtaac
    20581 ccaccttcac gctgcgcaca ttggtaatca ggcgcttata cataccggaa gtaaatatct
    20641 cagcaatcat ccgttggcgg gtaataatat ccggctcttt gcgcaccgca tggcaaacac
    20701 gcaatgcttc gtaggttatt tgctgatgaa attccggata aatcgtaatt ttgtcagcat
    20761 aatttcgatt taatttctcc agcaggcggg taatcttaat atagtgacgt tgataattaa
    20821 ggtttttatt cccttgtctg ttcaaccgac tcactgacgt attatgcaga taatatttat
    20881 ataatgattg ctcggtatat cgcgcacgca gcgcgttaaa cataaattct gttgtccaga
    20941 caatatcctg atgatgtaat ccggcaataa atttaatgtt atttttaaca ataacatcgc
    21001 ggcgataaac ccccatccag accacgtgtg tccaacggcg ggaagaaagt cccatccgca
    21061 gccagtccgg accagttaat acaccggttg agcgaaggcg atcggtaggg atggattgcc
    21121 aggtttcacc cgtttcacga aaacaccaat cagcgttgca ctgcgccacg tcgaggtcgt
    21181 cctctaacgc catggtcatc agcgtttcgt acatggtggg atacacctca tcgtcagcat
    21241 cgacaaaagc gacatatttt cccgttgcca cttcaatccc acgattacgc gccaccgatg
    21301 cgccagcatt cgcctgatgc aacaaacgaa cgtgcggata gttttctgcg taatgctttg
    21361 ctatttcaac agaattatcc gttgaaccat cgttaataat aatgatttcc agagcagtcc
    21421 atgtttgcgt aattaaagat tccatacaag tgcggaaatc atcgcccgca ttatataacg
    21481 gaataataac actaagttta ttggtgctgt tcatcataaa ttaccgacga cctctgaagt
    21541 tatcctgacg ttttacaggc tttgtattaa gaaactatta agcctgtgag atattatttt
    21601 taccggtgag agagtcacgt cagggtagag agataatgag agcagctata taagagatca
    21661 gcgcaattac gaactcttga ttacactgac aggattttac atatttaacg gattatcctc
    21721 aggcaaaagg gctggaatac cagcccttgt tcgtgttaat cccagctcag aataactttc
    21781 ccggactggc ccgaacgcat ggcatcaaag cccttctgga aatcatcgat agagaaacga
    21841 tgggtaatga tcggcgagag atcgaggcca gactgaatca gcgccgccat cttgtaccag
    21901 gtttcaaaca tctcacgacc gtaaatacct ttaatgaaca agcctttaaa gattactttg
    21961 gtccagtcga tagacatatc ggacggtgga atacccagca tcgcaatacg accgccgtgg
    22021 ttcatggtgt cgagcatggt acgaaacgct ggcggcgcac cggacatttc cagaccgaca
    22081 tcaaagcctt cggtcatgcc cagttctgcc atcacatcat taaggttttc tttagcgacg
    22141 ttaaccgcac gggtgatacc cattttgcgt gccagctcaa ggcggtattc gttaacatca
    22201 gtgatcacca cattgcgtgc accaacgtgt ttcgccaccg ccgcagccat aatacctatc
    22261 ggacctgcac cagagaccag cacatcctcg ccaaccagat cgaacgacag cgcggtatgc
    22321 acggcgttac cgaagggatc aaaaatggaa gccaggtcgt cggagatatt gtccgggatt
    22381 ttgaaggcgt tgaacgccgg aatcaccaga tactcggcga agcagcccgg acggttaaca
    22441 ccgacgccga tcgtgttgcg gcacagatgg gtacgaccgc cacgacagtt gcgacagtga
    22501 ccgcaggtga tatggccttc gccagaaacg cgatcgccaa tcttgaagcc tttcacttcc
    22561 tgaccaatac ctaccacttc accgacatat tcatggccca cgaccatcgg caccgggatg
    22621 gttttttgcg accactcatc ccagttatag atgtgaacgt cagtcccgca gatggctgtt
    22681 ttacggattt taatcagcag atcgttatgc ccgagttccg gtacaggaac gtcggtcatc
    22741 cagatgccct cttccgcttt cagtttggat aacgctttca tctcacaccc tcaggcgata
    22801 acgcccagtt gtttaccaat acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg
    22861 gtatgcgccg cagacatctg ggtacgaata cgcgcctgac ctttaggaac gaccggatag
    22921 aagaaaccgg tgacgtaaat gccctctttt tgcagctcac gggcaaattt ctgcgccact
    22981 accgcatcac caagcatgac cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc
    23041 gccgccgaca tttgctcacg gaactgacgc gcgttcgccc acagacggtc acgcagctcg
    23101 ctacccgctt cgaccatctc cagcactttg atggatgcgg cgacaatcgc tggtgccagc
    23161 gagttggaga acaggtacgg acgagaacgc tggcgcagcc actcaaccac ttctttgcgc
    23221 gccgcggtat aaccaccaga agccccgccc aacgctttac ccaacgtacc ggtaatgatg
    23281 tctacacgtc ccatcacatc gcagtattca tgggaaccac gaccattttc accgacaaaa
    23341 ccgaccgcgt gggagtcgtc taccatcacc agggcatcgt atttatctgc cagatcgcaa
    23401 acgcccttca ggttggcaat cacgccgtcc atcgagaaca caccatcggt ggcaatcagc
    23461 acatgacgcg caccggcttc acgcgcttct ttcagacgtg cttccagctc ctgcatatcg
    23521 ttgttggcat aacgatagcg tttggcttta cacagacgca caccatcaat aatagacgcg
    23581 tggttcagtg cgtcggagat aatggcgtcc tccgcgctca gcagcgtttc aaacaggccg
    23641 ccgttagcat caaagcagga agagtagaga atcgcatctt ccatccccag gaaggccgcc
    23701 agtttttgtt caagctcttt atggctgtcc tgagtgccgc aaataaaacg caccgaagcc
    23761 atgccgaaac cgtgagaatc cattcccgcc tttgccgccg caatcagatc cggatgattc
    23821 gccagtccga gatagttgtt ggcacaaaag ttaatgacgt ggcttccatc agccacagtg
    23881 atatctgctt gctgcgcaga cgtaataatg cgctcttctt taaacaaccc ttccgcccgt
    23941 gcggtttcca gatcgttggt taactgctga taaaattctc cacgcattgc gattctccag
    24001 acagggcaaa ttccagcaca tattacccaa acttataggt cgcgacgaga tgacgcgtta
    24061 acacttctgc aaaattcagg ataaataacg tccatcaatc tcacttttgg cgaatggctg
    24121 aagccaacgc cgtgtagtga tatgatagaa gcattcgtgt ctgagattgt ctctgactcc
    24181 ataattcgaa ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt atcggcagca
    24241 acatcgttaa agccctgaat gacaaaggca tcaccgatat tctggtggtg gacaacctga
    24301 aagacggcac caagtttgtg aacctggtgg atctggatat cgcggactat atggataagg
    24361 aagacttcct gatccagatt atggctggcg aagagttcgg cgatgtcgaa gcgattttcc
    24421 acgaaggcgc gtgctcttcc accaccgagt gggacggcaa atatatgatg gataacaact
    24481 atcaatactc caaagagctg ctgcactact gtctggagcg cgaaatcccg ttcctgtatg
    24541 cctcttccgc agccacttac ggtggacgca cctccgactt tattgaatcc cgcgaatatg
    24601 aaaaaccgtt gaatgtctac ggttactcaa aattcctgtt tgatgaatat gttcgccaaa
    24661 tcctgcctga agcgaactcg cagattgttg gcttccgtta tttcaacgtt tatggaccgc
    24721 gtgaaggcca taaaggcagt atggcgagcg tcgctttcca tctcaacacc cagcttaata
    24781 acggtgaatc accgaagctg ttcgaaggta gcgagaactt taaacgtgac ttcgtctatg
    24841 tgggcgacgt ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc ggcatcttca
    24901 acctcggcac cggtcgtgcg gaatccttcc aggcagtagc agatgctacg cttgcttatc
    24961 acaagaaagg ccaaatcgaa tacattccgt tcccggataa actgaaaagc cgctaccagg
    25021 cgttcactca ggcagatctg acgaatctgc gcgcggcggg ttacgataaa ccgttcaaaa
    25081 ccgttgccga aggcgtaacg gaatacatgg cctggctgaa tcgtgacgca taagagctct
    25141 gcatgaaaat actggtgatc ggcccgtctt gggttggcga catgatgatg tcgcaaagtc
    25201 tctatcgcac gctccaggcg cgctatcccc aggcgataat cgacgtgatg gcaccggcat
    25261 ggtgccgtcc attattatcg cggatgccgg aagttaacga agcgatccct atgcctctcg
    25321 gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg tcatattctg cgtgaaaagc
    25381 gctacgaccg cgcctacgtc ttacctaact ccttcaaatc cgcattagtg cctttcttcg
    25441 caggtattcc tcatcgcacc ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg
    25501 tacgcgtgct cgataaagaa gcctggccgc taatggtgga acgctatgtc gcgctggcct
    25561 atgacaaagg tattatgcgt accgcacaag atctgccgca gccattgtta tggccgcagt
    25621 tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca attttcgctt tcatcagaac
    25681 gtccgatgat tggtttttgc ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact
    25741 accactatgc ggagctggca aagcagctga ttgatgaagg ttatcaggtg gttctgtttg
    25801 gctcggcgaa agatcatgcg gcgggcaatg agattcttgc cgctttgaat acagagcagc
    25861 aggcatggtg tcggaatctg gcgggagaaa cacagcttga tcaagcggtt atcctgattg
    25921 cagcctgtaa agccattgtt actaacgatt ctggtctgat gcacgttgcg gcggcgctca
    25981 atcgtccgct ggttgccctg tatggaccaa gcagcccgga cttcacacca ccgctatccc
    26041 ataaagcacg cgtgatccgc ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg
    26101 cggagggtta tcaccagagc ttgatcgaca ttactcccca gcgcgtactg gaagaactca
    26161 acgcgctatt gttacaagag gaagcctgac ggatgcgggt tctgatcgtt aaaacatcgt
    26221 cgatgggcga tgttctccac acgctgcccg cactcactga tgcccagcag gcaatcccag
    26281 ggattaagtt tgactgggtg gtggaagaag ggttcgcaca gattccttcc tggcacgctg
    26341 ccgttgagcg agttattcct gtggcaatac gtcgctggcg taaagcctgg ttctcggctc
    26401 ccataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca agcaaagaac tatgacgcag
    26461 ttatcgacgc ccaggggctg gtaaaaagcg cggcgctggt gacgcgtctg gcgcatggcg
    26521 taaagcatgg aatggactgg caaaccgctc gcgaaccgtt agccagcctg ttttacaatc
    26581 gtaaacatca tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcca
    26641 aaagtctggg ctatagcaaa ccacaaaccc agggcgatta tgctatcgca cagcatttcc
    26701 tgacgaacct gcctacagat gctggcgaat atgccgtatt tcttcatgca acaacccgag
    26761 atgataaaca ctggccggaa gaacactggc gagaattgat tggtttactg gctgattcag
    26821 gaatacggat taaacttccg tggggcgcgc cgcatgagga agaacgggcg aaacgactgg
    26881 cggaaggatt tgcttatgtt gaagtattgc cgaagatgag tctggaaggc gttgcccgcg
    26941 tactggccgg ggctaaattt gtagtgtcgg tggatacggg gttaagccat ttaacggcgg
    27001 cactggatag acccaatatc acggtttatg gacctaccga tccgggatta attggtgggt
    27061 atgggaagaa tcagatggtt tgtagggctc cggggaatga gttgtctcaa ttgacagcaa
    27121 atgctgttaa gcaattcatt gaagaaaacg ctgaaaaagc tgccatgatt taaattacaa
    27181 ctctttaata gataattttg aaataaaatc agcttccttg ctgattttat ttcacatatt
    27241 cattacttat ctaataaaca ttggtccgat tgtactttaa aataagcaca aagcaatatt
    27301 atggtgacaa acacagcagt cggaaccggt ttagaaaaga gtattacatc acttaggcct
    27361 actaccagta gtgaaaaaag caagattaaa atataaggtt ctttagcagt tcttaaggca
    27421 tatatgaata ttgctgagaa ggctaaaatt gttaatgcta ctccaggaat accacgcgtt
    27481 gataacgtat ctatgagatc gttatgcaaa tgagaatcta caaagggtaa agcaccactt
    27541 aatctaggct ctttctcttc tagttcatgt attttttccg cacgtttttc cagtgattgt
    27601 cctattggag aatatgtttt taaaccaact tcatacattg ccagacgggc accgactgaa
    27661 gttctggtgt tatcatgcga ataattaact aaatccctat ttaagtcatt cattctcgta
    27721 agtaatgtat cttt
//
LOCUS       DAZFRE010000072        27011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFRE010000072 DAZFRE010000000
VERSION     DAZFRE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.072"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            153..899
                     /gene="mipA"
                     /locus_tag="ACHKUI_003318"
     CDS             153..899
                     /gene="mipA"
                     /locus_tag="ACHKUI_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6599465.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGIAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            989..1843
                     /gene="yeaE"
                     /locus_tag="ACHKUI_003319"
     CDS             989..1843
                     /gene="yeaE"
                     /locus_tag="ACHKUI_003319"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6599466.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1894..2778)
                     /gene="yeaD"
                     /locus_tag="ACHKUI_003320"
     CDS             complement(1894..2778)
                     /gene="yeaD"
                     /locus_tag="ACHKUI_003320"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6599467.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPLCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVVLTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(2862..3857)
                     /gene="gapA"
                     /locus_tag="ACHKUI_003321"
     CDS             complement(2862..3857)
                     /gene="gapA"
                     /locus_tag="ACHKUI_003321"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6599468.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            4199..4612
                     /gene="msrB"
                     /locus_tag="ACHKUI_003322"
     CDS             4199..4612
                     /gene="msrB"
                     /locus_tag="ACHKUI_003322"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6599469.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            4654..4926
                     /gene="yeaC"
                     /locus_tag="ACHKUI_003323"
     CDS             4654..4926
                     /gene="yeaC"
                     /locus_tag="ACHKUI_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6599470.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            5296..6372
                     /gene="ydjL"
                     /locus_tag="ACHKUI_003324"
     CDS             5296..6372
                     /gene="ydjL"
                     /locus_tag="ACHKUI_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6599471.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            6399..7778
                     /gene="ydjK"
                     /locus_tag="ACHKUI_003325"
     CDS             6399..7778
                     /gene="ydjK"
                     /locus_tag="ACHKUI_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599472.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            7795..8838
                     /gene="ydjJ"
                     /locus_tag="ACHKUI_003326"
     CDS             7795..8838
                     /gene="ydjJ"
                     /locus_tag="ACHKUI_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO6599473.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGAQVEPAAVGMHA
                     AMLANVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            8859..9695
                     /gene="ydjI"
                     /locus_tag="ACHKUI_003327"
     CDS             8859..9695
                     /gene="ydjI"
                     /locus_tag="ACHKUI_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6599474.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            9700..10647
                     /gene="ydjH"
                     /locus_tag="ACHKUI_003328"
     CDS             9700..10647
                     /gene="ydjH"
                     /locus_tag="ACHKUI_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6599475.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            10657..11637
                     /gene="ydjG"
                     /locus_tag="ACHKUI_003329"
     CDS             10657..11637
                     /gene="ydjG"
                     /locus_tag="ACHKUI_003329"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO6599476.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            11774..12532
                     /gene="ydjF"
                     /locus_tag="ACHKUI_003330"
     CDS             11774..12532
                     /gene="ydjF"
                     /locus_tag="ACHKUI_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6599477.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            12650..14008
                     /gene="ydjE"
                     /locus_tag="ACHKUI_003331"
     CDS             12650..14008
                     /gene="ydjE"
                     /locus_tag="ACHKUI_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599478.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMHWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(14101..14742)
                     /gene="pncA"
                     /locus_tag="ACHKUI_003332"
     CDS             complement(14101..14742)
                     /gene="pncA"
                     /locus_tag="ACHKUI_003332"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6599479.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(14753..15769)
                     /gene="ansA"
                     /locus_tag="ACHKUI_003333"
     CDS             complement(14753..15769)
                     /gene="ansA"
                     /locus_tag="ACHKUI_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6599480.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGALIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASYRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(15936..17792)
                     /gene="sppA"
                     /locus_tag="ACHKUI_003334"
     CDS             complement(15936..17792)
                     /gene="sppA"
                     /locus_tag="ACHKUI_003334"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6599481.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SIRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            17953..18504
                     /gene="ydjA"
                     /locus_tag="ACHKUI_003335"
     CDS             17953..18504
                     /gene="ydjA"
                     /locus_tag="ACHKUI_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6599482.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            18621..19664
                     /gene="selD"
                     /locus_tag="ACHKUI_003336"
     CDS             18621..19664
                     /gene="selD"
                     /locus_tag="ACHKUI_003336"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6599483.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            19669..21615
                     /gene="topB"
                     /locus_tag="ACHKUI_003337"
     CDS             19669..21615
                     /gene="topB"
                     /locus_tag="ACHKUI_003337"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6599484.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKHFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGIVASGGGA
                     VKKAPNKRVKKAAKPKTDNTDSAN"
     gene            21982..23682
                     /locus_tag="ACHKUI_003338"
     CDS             21982..23682
                     /locus_tag="ACHKUI_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HFO6599485.1"
                     /translation="MIIGIDLGTTNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDG
                     MVLTGEAARDLQLIKPQNCASNFKRMMGTSKTLKLGGREFRAEELSSLILRQLKEDAE
                     NYLGEEVTEAVISVPAYFGDMQRKATKAAATMAGLNVERFINEPTAAALAYGLHNKDD
                     EHQFLVFDLGGGTFDVSILGLFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPE
                     NIEWREPTLQRHLRIEAERVKRVLSVRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFF
                     ERIHMPLERAIRDAKINISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVI
                     AQGAAIQAALKARHSELRDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIP
                     CSRVRAFYTAHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTY
                     DINGILDVDISVPLTGAKNSLVIEQNPGALTAEQIQQSLSKLSLLKIHPRDEQINQAF
                     IARLDNLYQLTLAETRDWISNCSRHFSYLLEKQDPDQLADFRTKVEPTLDSLERERLQ
                     "
     gene            23679..25622
                     /locus_tag="ACHKUI_003339"
     CDS             23679..25622
                     /locus_tag="ACHKUI_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFO6599486.1"
                     /translation="MNCWEILALQPGAEERDIKRRYAQLVKNCRPEDDPAAWQQLHDA
                     YEQALRYDDEDQWQEDEEEQPINMSRGIVHPRPVMTFRALNTFSGHPLPDVTIKSEGE
                     VTWQAVLDMLCEENEAIPEHAPMQLAKALSFLEKKDLSSRMCFEEALLLHLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPVIQTEINESCLLYYRIEKQITPFFASNLSPLEEMENGK
                     EMATIYQSLKDNEESLKWFDIAVLNKVAEFELSDNYMALLIECLSYSLRKEKMIRKWK
                     LTERFNNLAMQHHSGYKKLLNAIKLKGLFYYPSSVYQSCLHAAEQGYSLAWYSLGKMY
                     YEGDEVSQDLKLAFNWFTRAAQHNVIDALYALGIMYSDGRGTDKNICEARKWFLLAAQ
                     NGNTSAQYELARISRFAVEPLRNYEEALQWYLSAATQGHEVAQYELGQMYIQGIGVER
                     DEVQAHRWFLQSAEQGYLHAQYHTARLYSGSESISQDQEKALYWFTKAAKNGADGAGD
                     AMYELGKYYLTNNDDPENNAEATQWLTGAAQRGRIEAIFLLAEMYLYGTKDTIKDENH
                     ALHWYEKAARLGSTEAQHQTAAMYAQGTGTKIDNKQAWMWLTIAGNNNPWIEKDALRC
                     LMSEQDIQDAEQTAANCKRLLRI"
     gene            complement(25678..27003)
                     /gene="gdhA"
                     /locus_tag="ACHKUI_003340"
     CDS             complement(25678..27003)
                     /gene="gdhA"
                     /locus_tag="ACHKUI_003340"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6599487.1"
                     /translation="MESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSL
                     LERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKF
                     LGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPA
                     GDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLK
                     RHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARL
                     IEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGV
                     KAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKV
                     DARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
BASE COUNT         7010 a         6153 c         6905 g         6943 t
ORIGIN      
        1 gctaaattca tttggctgta agcgtggtgt catctgcgtc aggaaaatta aacagttact
       61 ataaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc
      121 ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg
      181 gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt tccctgggcg
      241 caggcgtagg tgtcgttgaa cacccatata aagattacga taccgatgtt tacccagtac
      301 cggtaatcaa ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc
      361 tgtggaatga cgcaacggac aaactttcaa ttaccgctta ctggtcgccg ctttacttca
      421 aagctaaaga cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga
      481 tggctggtct gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg
      541 gcgataccct ggataacagc aacggcatcg tctgggatat ggcctggttg tatcgttaca
      601 ccaacggtgg cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga
      661 acgaatacta ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtggctata
      721 acccgaacga cagctggagc ccttacctgg agctgagcgc cagctacaac ttcctcggtg
      781 actggagtgt ttacggtatc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc
      841 caatggtgga taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat
      901 aatgcatttt tccagggcat tttgtgcata aaaacagggc gtaatgccct gttttgcatt
      961 acggtggtgc aatacctggg aggaagcaat gcaacaaaaa atgattcaat ttagtggcga
     1021 tgtctcactg ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg
     1081 taaaacagaa gttgctgcac tacgcgcggg cattgaactc ggtttaaccc tcattgatac
     1141 cgccgaaatg tatgccgatg gcggtgccga aaaagtggtt ggggaagcat taatcggtct
     1201 gcgtgagaac gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc
     1261 gataaatgca tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt
     1321 attacactgg tctggcagtt tcgctctcga agagactgtc gcagcgatgg aaaaattgat
     1381 cgcccaggga aaaatccgcc gctggggcgt ttctaacctt gattatgccg atatgcagga
     1441 actctggcag ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg
     1501 ttcacgaggg attgagtacg atctacttcc ctggtgccag aaacagcaga tgccggtgat
     1561 ggcttacagt ccattagctc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt
     1621 agtcaacgaa attgcacatg ctcacaatat cagtgcggca caagtattgt tggcgtgggt
     1681 aatcagccat cagggtgtga tggcgatccc aaaagcggca actgtcgcac atgtccaaca
     1741 aaatgcggct gtgcttgagg ttgaactttc gtcagcggaa ttagttatgc tggataaagc
     1801 gtatccggca ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga
     1861 tgcatcgcac ccggcacaca atattaacgc aaattaacgt ttcgcaacgc gaatggattg
     1921 cgccagatgt gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc
     1981 tacacaaaca aaggtcttgt agccatcatc cggcatatcg cccatgctaa ctgaaagcgc
     2041 cggtcctggg ttccagccta caacgttcag atgatgctgg tggcctacgg cgataatacg
     2101 attcagcgct tcgtcattaa ttacgctgca atcttgcgga ttcagataca cgcgatcggt
     2161 acggtcaggg aaggtctgaa taccgtcggt cagtacatct tctttcgcgt catttacttt
     2221 atcaatgaag cgatcgccta gtccactgac gcttacctta gcgatatcac ccacgttaaa
     2281 gtaggtatgc agagcagagg tagtttcaaa ttcgccgtgt gcttcaagat cgatttcaca
     2341 ggttttaccc acgtggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt
     2401 ctcttcgctt tgtgtcaatt caaaagttag cactacacca tcagcatctt cacgatgtga
     2461 tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgcagc
     2521 cggaccaaac cacggccagc aaagcggtat gccaccgcga atggcgacgc catttttgaa
     2581 cggtgtgttg ttacttaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag
     2641 gtgtgcgccc tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc
     2701 cagttcatcc agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc
     2761 aaaaattttc ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag
     2821 cgaccgaagt cgctcttttt agatcacaga gtcatctcaa cttatttgga gatgtgagcg
     2881 atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc
     2941 acgaagttgt cgttcagagc gatacccgct ttagcatcga acacggaagt gcaaacttcg
     3001 ccgttgaaat cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg
     3061 ccttcagcag cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga
     3121 cgaacggtca ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc
     3181 agtttgccat tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaagac
     3241 gggatgatgt tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca
     3301 acggttttct gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg
     3361 aagttatcgt tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg
     3421 gaaacgatgt cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg
     3481 ttgtctttag acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga
     3541 gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga caacgtcaac accaacttcg
     3601 tcccatttca ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg
     3661 atcagatgac cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat
     3721 ttcagcatgt atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg
     3781 tcagaacgtt tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata
     3841 cctactttga tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta
     3901 aaattacaaa aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa
     3961 tcctgtgcct aagcattacg cgactgactc gcctcactct tcctttgggc ttgagaccac
     4021 atggggtcgg cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg
     4081 atttaggtgc agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt
     4141 aatgttttgt tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat
     4201 ggctaataaa ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttctacgt
     4261 gacgcagaat catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga
     4321 cggcgtatat cactgtttga tctgcgatgc cccgctgttt cattcccaaa ccaagtatga
     4381 ttccggctgt ggctggccca gcttctacga accggtgagt gaagaatcta ttcgttatat
     4441 caaagacttg tcgcatggaa tgcagcgcat agaaattcgt tgcggtaact gcgatgccca
     4501 tctgggccat gtctttcctg acgggccgca gccaacgggc gaacgttatt gtgttaactc
     4561 tgcttcttta cgcttcaccg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga
     4621 ttcagcaact tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc
     4681 atgacgcctg aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat
     4741 ggcgttacgt tgaccgagga acaaaaagag aactgcctgc aactggtgat gctgtggcaa
     4801 gcccgccata ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg
     4861 aagagcaaac agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt
     4921 aagtaagtgg tttatcccct ttttgccgcg gttataaagg ggatagccaa caatgacccc
     4981 acgactcatc tcaattatct cgccccgacc tggctaagtc cctgtccgat ctcttgcagt
     5041 cgcgttcaca cctgaatgac ttctcctgaa cttaagtctc aggcctattt ggccgggaat
     5101 ccctctcgaa tttgctcttt ttgcgtcccg ccttccatca acctgtaaca gccccagcca
     5161 ttttctgcgt aattgtgatc taagttgtaa aacgcagatc attatctgtg ttttacagat
     5221 ttgatgttga cctcaaaaat catgtgggta agtatgatga ccatctaagc aaaacaaaac
     5281 acagaggatg acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag
     5341 atgattgatg tcccacaacc catttgtggc ccggaagatg tcgtgattga aattaaagcc
     5401 gcggcaatct gcggcgcaga catgaagcac tacaatgtcg atagcggttc ggatgagttt
     5461 aactctatcc gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa
     5521 gactggaaag tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgc
     5581 ccggcctgtg aacaaggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat
     5641 aacaatacct ggggcggtgg tttttccaaa tattgtcttg ttcctggtga aattctcaaa
     5701 attcatcgtc atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta
     5761 cttgacccta tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttccaggt
     5821 caggatgttg tcgtcatcgg cactggccca ctcgggctgt tctccgtaca aatggcgcgg
     5881 attatggggg cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc
     5941 ccggttgcaa aagaactggg tgcgacggca gtagtaaatg gttctaccga agatgtggtg
     6001 gcgcgctgcc agcaaatttg tggcaaagac aatctgggac tggtgattga atgctccggt
     6061 gccaatatcg cactgaaaca agccatcgaa atgctccgcc caaatgggga agtagtacgc
     6121 gttggaatgg gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaacaaa
     6181 agcatcattg ggcatatggc ctatgactcc acctcatggc gtaacgctct ccggctatta
     6241 gccagcggcg cgatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg
     6301 cgcgaagggt ttgatgcgat ggtcgataaa actgcaatca aagtgatcat gacttacgac
     6361 tttgatgaat aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc
     6421 attgtggtgc acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca
     6481 tcgtcgcgtt tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc
     6541 agctgaaagc gttgggctgg acagataatt ccaccactgc cacattctca gcaatcacga
     6601 ccgccggaat gtttctcggt gcgctggtgg gcggcatcat cggtgacaaa accggtcgca
     6661 gaaatgcgtt tatcctctat gaggccattc atattgcttc gatggttgtc ggtgctttct
     6721 caccgaatat ggatttcctt attgcctgcc gttttgtgat gggcgttggg ctgggagctt
     6781 tactggttac actgtttgct ggtttcaccg aatatatgcc cggaagaaat cgcggaacgt
     6841 ggtccagtcg ggtttccttt attggtaact ggtcatatcc gctctgttca ttgattgcga
     6901 tgggactcac gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttatccctg
     6961 caatattgtc acttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct
     7021 ggctggaatc gcgcggacgt tatcaggaag ccgagaaagt catgcggagt atagaagaag
     7081 gcgtcatacg ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag
     7141 caccacaagc ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat
     7201 taggctcttg tgtgctgatt gccatgaacg ttgtgcagta cacactaatt aactggttgc
     7261 caacgatatt catgacccag ggaattaatt taaaagactc gattgtttta aataccatga
     7321 gtatgtttgg cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc
     7381 gtaaaacaat gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac
     7441 tgcaaaccag tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata
     7501 tgtacgtttg ctatgcctcg gcagtgtatg tccctgagat ctggccgaca gaggccaaac
     7561 tccgtggctc cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcgccttatg
     7621 ccgttgcagt gctgctcagt agttatggcg taacgggagt ctttattctt ctgggggctg
     7681 tttcaattat tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg
     7741 ttgaaagttt aagtattgat gcagtagcca ataaataata ctggtggatt caaaatgaaa
     7801 aattcaaaag caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacct
     7861 gtgcctaaag aagatgaagt tttgattaaa gtggaatatg tcggtatttg tggttcagat
     7921 gtacatggtt ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc
     7981 ctgggtcatg aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa
     8041 ccgggggatc gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg
     8101 gaaggcaaat ataacatttg cccggacgtt gattttatgg cgacacaacc caactaccgc
     8161 ggcgcattaa cgcactatct gtgtcatccg gagagcttta cttacaaact gccagacaat
     8221 atggacacga tggaaggggc gcaggtggag cctgccgcag tcgggatgca tgccgcgatg
     8281 ctggcaaatg ttaaacctgg taagaagata attattctgg gagcgggttg tattggtttg
     8341 atgacgttgc aagcgtgcaa atgcctggga gcaacggaaa ttgccgtcgt tgatgtgctg
     8401 gaaaaacgtc tgacaatggc ggaacagctc ggcgcgacag tggttattaa cggcgcaaaa
     8461 gaagacacta ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttt
     8521 gaaacagcgg gttctgcggt caccgttaaa caggcacctt atctggtcat gcgcggcggt
     8581 aaaattatga ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat
     8641 cgcgaagtca ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa
     8701 gctatttctt cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg
     8761 gatgtacaac aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt
     8821 attaaaatta gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt
     8881 gggaaaacga tgccacaaat aaacattatg caattgccca ttttaacgta tggaatgcag
     8941 aaatgttgat gggcgttatc gacgcagccg aagaagcgaa atcaccggtt attatttctt
     9001 ttggcacagg ttttgtcggc aacacctcat ttgaagattt ctctcacatg atggtatcaa
     9061 tggcacaaaa agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtatggaga
     9121 ttattcataa cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg
     9181 atttcgaaga aaatattcgt ttaaccaaag aggctgttga tttcttccat ccgctgggta
     9241 ttccggtaga ggcggaatta gggcatgttg gtaatgaaac cgtttatgag gaagcgctgg
     9301 cgggctatca ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg
     9361 actcattggc tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccacaattga
     9421 actttgaggt cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg
     9481 catcagggat cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca
     9541 acattcatac ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc
     9601 ctttcctgca tttagaacgt gaagtgcgta aagctgtaaa agaacgggca ttggataaaa
     9661 ttaaactgtt cggttctgat ggcaaagcgg aataataata tggataatct cgacgttatt
     9721 tgtataggtg ccgctattgt tgatattcca ttgcaaccgg tcagtaaaaa tatctttgat
     9781 gtggattctt atcctcttga aagaatcgca atgaccaccg ggggtgatgc aataaatgaa
     9841 gcaacaatta tttctcgtct gggccatcgc acagcattaa tgagtcgtat tggtaaagat
     9901 gccgcagggc aatttattct cgaccattgc cgcaaagaga atattgatat tcaaagcctg
     9961 aagcaggacg tcagcataga tacctctatt aacgttggac tggtgacgga ggatggtgag
    10021 cggacctttg tcacaaaccg taacggcagt ctgtggaaat tgaatattga cgatgttgat
    10081 tttgcgcggt tttctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta
    10141 ttggatggta gagcgctaac agaaattttt acgcaagcta aagcccggca gatgattatc
    10201 tgtgccgata tgatcaaacc gcggttgaat gaaacgctga atgatatttg cgaagcattg
    10261 agttatgtcg attatctgtt tcctaatttt gccgaggcaa aattactcac cgggaaagag
    10321 acactggatg aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa
    10381 acgggtaaag acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc
    10441 gcaggaataa ccgctatcga caccattggt gcgggcgata actttgcttc aggttttatt
    10501 gcagcactgt tagaaggtaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg
    10561 gctatctcgg ttctaagcat cggcgccacc actggtgtaa aaaacagaaa gctggtcgaa
    10621 caattgctgg aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcaca
    10681 acggatataa cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca
    10741 tggaatggcg atctcgatcg acaaatatgc attgatacga ttcttgaagc ccatcgttgc
    10801 ggcattaacc tgattgatac tgcgccagga tataactttg gcaatagtga agttatcgtt
    10861 ggtcaggcgt taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt
    10921 gtctgggaac gaaaagggag tttattcaac aaagttggcg atcggcagtt gtataaaaac
    10981 ctttctccgg aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat
    11041 tacatcgata tctacatgac gcactggcag tcggtgccgc cattttttac gccgatagct
    11101 gaaactgtcg cagtgcttaa tgcgttaaag gccgaaggga aaattcgcgc tataggcgct
    11161 gctaacgtcg atgctgacca tatccgcgag tatctgcaac atggtgaact ggatattatt
    11221 caggcgaaat acagtatcct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt
    11281 gataatggca ttgtggtaca ggtctactct ccgctagagc agggattgtt gaccgggacc
    11341 atcacgcgtg attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa
    11401 aacatgctga aagtgattga tatgcttgaa cagtggcagc cactctgtgc tcgttatcag
    11461 tgcacaattc ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt
    11521 cttagtgggg ctactgcgcc ggaacaggta cgtgaaaatg tcgcggcact gaatatcaac
    11581 ttatcggatg cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata
    11641 ctcaacacga ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaacag
    11701 gttattttga aagtaaacat cggttatgcg ataattgcgc taatgtgatg taaaaagata
    11761 caggggtgta atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa
    11821 cgataaaaag gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat
    11881 tcgccgcgat cttgagaagt tggaagatga aggctttttg accagaacct atggtggtgc
    11941 tgttttaaat acagcgatgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt
    12001 ttatgaagag aagcagctta ttgcacgcaa ggcactcccc tttatcgaca ataaaaccac
    12061 catggcagcc gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag
    12121 tgacctgacg ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat
    12181 taaagtcgtt tcaacgggtg gggaactaaa caaaaataca ctttcactgc aaggaagaat
    12241 tactaaagag atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct
    12301 tgatattaac agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat
    12361 gatccgtcag gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgcaaagc
    12421 ttttgtccag ttagctgact ttagtcatat taattacata ataactgata aatcaccggg
    12481 tgcagagtgg atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca
    12541 cacattgtca atattatttg ttcctgcgcc tggaaataaa atgatgataa agatgcggca
    12601 agtgaatttg ccgcaattgc tacctttttt agcaaatccg aggcacccaa tggaacaata
    12661 tgatcaaatt ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt
    12721 tggtataata agctttagtc tgttattaac ggggtttttg agttactccg gtaatgtcgt
    12781 cttagcaaag ctggtaagca atggatggtc aaataatttc ctcaatgccg cctttacctc
    12841 ggcattaatg tttggttatt tcatcggctc tcttaccggt gggtttattg gtgactactt
    12901 tggacggcgc agggcgtttc gcataaatct tctcatcgtc ggtattgcgg caacaggggc
    12961 cgcttttgtc cctgatatgc actggctcat cttctttcgc ttcctgatgg gaacaggaat
    13021 gggggcgctg attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg
    13081 tggaaaatgg tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc
    13141 gataggcgtg gtggttatcg ctttttttag ttggcggata atgtttctgc tgggcggtat
    13201 tggcatactg ttagcctggt ttctctcagg taaatacttt attgagtcgc cacgatggct
    13261 ggcagggaaa gggcaaatcg caggtgcaga aagccaactt cgtgaagtag agcagcaaat
    13321 tgaaagagag aagagtattc gtttaccccc gcttactttg aaccagagca acagcaaggt
    13381 taaggtaatc aagggtacct tctggctcct gtttaaaggg gaaatgttac gacgtacatt
    13441 agtcgcgatt actgttttaa ttgcaatgaa catttcgctt tataccatca ccgtatggat
    13501 accgaccata tttgttaact ccggcattga tgttgataaa tcaatattaa tgaccgctgt
    13561 tattatgatt ggcgctccgg tagggatatt tattgcggca ttaattattg atcattttcc
    13621 tcgtcgatta tttggctctg ccttacttat tattattgct gtgttaggct atatctattc
    13681 aattcagact acagagtggg cgattttaat ctatggcctg gtgatgatct tctttttata
    13741 catgtatgtg tgcttcgcgt cggcggttta tatcccggaa ctttggccaa cgcatttacg
    13801 cctgcgtggt tcgggtttcg ttaatgccgt cggacggatc gtcgcagtct tcacgcccta
    13861 tggcgttgcg gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt
    13921 catgttagtg ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc
    13981 gctggaagag atttctgagg tgaattaaat ctttcgttga tggcgtcaat taccagatgt
    14041 atcactgatg tgttttatct ggcctacaat gccagtacag aattgtaggc cagcgtaaaa
    14101 ttactcctgc gtttcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat
    14161 ctccataaac gcgtgcgcac tgtcctgtgg ctggatattt acgccacgac aaccatcggt
    14221 aatcacgttc accttatagc ctaactgtaa cgcgtccagc acggtaaact taacgcaata
    14281 gtcagtagcc agacccataa cgatcaattc attgattaca tgagcgcgca accagtcatc
    14341 gagcgcagtt ttctgccgac ggccgttatc aaaaaaggca ctgtaactgt caactaaagg
    14401 attttcgcct ttatggaaca ccgctgcgat cgctttttgc ttcagtaacg gatgtaattg
    14461 tgcgccttca ctgttctgca cacagtgatc gggccagaag gtttgtggca aaccgtcgag
    14521 ttggcccggc gtataaggct ctacaccgtg ctgactggca aaactgccgt gattcgccgg
    14581 gtgccagtcc tgactggcga taaccgcttc accgcgcgac tggcaccagt caatcaggcg
    14641 gttagcgaca tccaccgtac tgtcaccttc cggcacggcg agcgcgccac cagcacagaa
    14701 atcattttgt aaatcgacca gtaacagggc gcgagggggc attgcagtct ccttaatcat
    14761 ccggcgtcag ttcgccacgc aggttttggc tcatggcctt gcgaatggtt tcagtatcaa
    14821 gttcctggct aagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac
    14881 cgccaataac gccggcatgg gcgagggcgt taccagtggc ataaccaccc atgttcactt
    14941 taccggacat acattgtgtc aggttgacca ccacaatacc gcgatagctg gcttcttgta
    15001 attcctgcag gaaggctttg ttttgtggcg cattacccac gccatatgag cgcagaatca
    15061 atgctttcac aggttggcgc agaaaattgc gcacgacgtc agcagaaatc cccggataaa
    15121 tcgttactac gccaattggt tgcggcgtga ttggatgaac gatcaatgcc ccttcaccgt
    15181 gcggggcggg tggcgtattc aaacggcgaa tatggatacc tgcttccagt aacggaggaa
    15241 ggtttggaga ggcaaacgca tcaaaaccat cggcatgggc tttggtggtt ctgttgccac
    15301 gatacagtcg attattaaaa aagagagtta cttcgttgat aggataattc gccgcaacgt
    15361 acaacgcatt cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg
    15421 accctgtcac aatgaccggt ttaccgagat tctcgagcat gaacgacagt gcagaggcgg
    15481 tatacgccat cgtgtctgta ccgtgcagaa tgacaaaacc atcataatcg tcatagtgcg
    15541 ctttaatatc ttcagcaata tgctgccagt cttctggcgt catatcagaa gaatccatca
    15601 atggcgtata ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca
    15661 gcgccagttg gcgctgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca
    15721 tcccgatggt cccgcccgtg taggcgacgt aaattgactt cttttgcatg atattgatat
    15781 gaatgatatc gaaagagcaa aagtatatac gtgaggcagg gggagcgcac ctgatactat
    15841 gaggataagg aaatatgtgg cgagacagcg ggaagaggat tcgtcgacgt aaaaagtaat
    15901 gtggccgacg atctgtcggc cacttagtac aagacttaac gcacgttggc gcaggtcagg
    15961 caaaacgcat aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta
    16021 acggtagagg ctaccgaggc cagcggtgca ggtaacatgg cctggaacgc atctggcaac
    16081 attgcccgga tagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca
    16141 acgtagtatt ccagatgcca ctgtttcact tttgccagct ctgctgcttt ggcgaccgca
    16201 tcatcgaaat ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag
    16261 acgtgaccct gggcgatttt atcaatctgc tccggcgttg aatgacgcgc atcagcaacc
    16321 agcgtgataa agcgtttata gccattctca atgcttaact gcatcatctg ctgcgcttcc
    16381 ggcggcagtg ccctggtgat agaaacatct gccagcggtg aagttgagac accatcagta
    16441 tgaacaccaa tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata
    16501 gaaccggtca gggtgctggg gttagccaca atgtaattag ctggtgtgga aatccagtaa
    16561 ccaccagatg ccgccatgcc acccatcgat acaaccacag gcttacccgc tgcccgggct
    16621 gctgccagtt cagcgcgaat cacttcagac gcggtaacgc tgccgccggg actattaaca
    16681 cgcagaacaa tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg
    16741 gtatcaccgc caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag
    16801 acgacaccga tactgtcacc agtatctgcc ggcgttttca atgcgtaatc gtaataactg
    16861 atggcgcgat aatttttatc agttttactc cagccgaact ctttggtcag agttttttcg
    16921 atttcggcac tcgatgccag tgcatcgacc agcttgtttt ccagcgcata tttcgcggta
    16981 tcgccaccgg ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga
    17041 gcagggatct ggcggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc
    17101 cagcggctgt cagcttcgcg ggctgccggc gacatatcat cacgaataaa cggttcaacg
    17161 gcagatttat acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagtaac
    17221 gatttgtagt acagaccgtt ggtggcaaag ccgtgcagat caaccacgcc ttgcggagac
    17281 agccaaattt tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca
    17341 acggcataaa ccggtttccc gctatcacga aactctttca gagctttgcc gatgtactgc
    17401 atagacggct ggtcgccgcc tgcgaagttt ttcagatcca tcacaatacc ggtgatattg
    17461 cggtcgtcct tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga
    17521 cgatcggaac tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtcg
    17581 ggtttatcga cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct
    17641 ttcgaatcac caccactgac ctgcatccag atccccacac caaccagcac gagaaaaata
    17701 aagaacaggt taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa
    17761 aatccggcaa taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg
    17821 tttcaggcgc aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt
    17881 gcggactggg ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcatgaca
    17941 ggagttaatc aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg
    18001 gctgaacccg cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg
    18061 ccggaccata agtccatgca accgtggcat ttctttgtga ttgaagggga agggcgcgag
    18121 cgtttcagcg ctgtactgga gcagggggcg attgctgccg gtagtgataa caaagcaatc
    18181 gacaaagcgc gtaatgcgcc gttccgcgca ccgctcatca taacggtggt ggcgaaatgc
    18241 gaagaaaatc ataaagtccc gcgctgggaa caggaaatgt ctgcgggatg cgcggtcatg
    18301 gcgatgcaaa tggcagcagt tgcccagggg tttggcggca tctggcgcag tggtgcatta
    18361 actgaaagtc cggtagtccg tgaagcattc ggttgccgtg agcaggataa aattgtcggt
    18421 tttctctacc tcggtacgcc gcagttgaaa gcatctacgt cgataaacgt cccggacccg
    18481 acgccgtttg tgacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg
    18541 cgcaggaatt cagacactct cacttatcac ttcatggaat gagggttacc atacccggat
    18601 tgcattgaca ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg
    18661 gcgctggttg cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc
    18721 aggcgaagtt tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg
    18781 tatacgatct gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg
    18841 ttgataatcc tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatctttg
    18901 ctatgggcgg caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt
    18961 ccccggaaat tgcccgcgaa gtgaccgaag gtggacgcta tgcatgccgt caggcgggga
    19021 ttgcgctggc tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa
    19081 cggggatcgt accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac
    19141 tgttcctgac gaaaccgctg gggattggcg ttcttaccac ggctgagaaa aaatcactgc
    19201 tgaaaccaga acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg
    19261 cgtcctttgc caacatcgaa ggcgtaaaag cgatgaccga cgttacgggt tttggtttac
    19321 tggggcattt aagcgaaatg tgtcagggcg caggtgtgca ggcgcgcgtc gactatgacg
    19381 cgatcccgaa attgccgggg gttgaagagt acattaagtt gggcgcagtg cctggcggca
    19441 ccgaacgtaa ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg
    19501 atctgctgtg cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag
    19561 aaaatgaggt caaagctaca gccgccgagt ttggcattga actgacggcg attggcgaac
    19621 tggtgccagc gcgcggcggt cgtgccatgg ttgagatccg ttaattcaat gcggttgttt
    19681 attgccgaaa aaccgagtct ggcgcgcgcc attgctgatg tcctgcctaa accgcaccgg
    19741 aaaggcgatg gctttatcga gtgcggtaat ggtcaggtgg tgacctggtg tatcggtcac
    19801 ctgcttgagc aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg
    19861 gatttgccaa ttgtcccgga aaagtggcaa ttacagcccc gaccctccgt gactaaacaa
    19921 cttaatgtca tcaaacactt cctgcatgaa gccagcgaaa tcgttcacgc cggagacccg
    19981 gatcgcgaag ggcaactgct ggtggatgaa gtgctggact acctgcaact agcaccggaa
    20041 aagcgccagc aggtacagcg ttgtttgata aacgacctga acccgcaggc ggttgagcgg
    20101 gcgatcgatc gtcttcgctc caatagcgag tttgtaccgc tgtgcgtttc tgcactggcg
    20161 cgggcgcggg ctgactggct gtacggcatt aatatgaccc gtgcgtatac cattctcggc
    20221 cgcaatgccg gttatcaggg cgtgctttcc gtgggacgcg tgcagacgcc cgtactcgga
    20281 ctggtggtgc gccgcgacga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc
    20341 aaagcacata tcgtgacacc tgcagatgag cggtttaccg ctatctggca accgagcgaa
    20401 gcgtgtgaac cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat
    20461 gtggttaacc gcattagtgg tcaaccagct attgtcacca gctataacga taaacgggaa
    20521 tcagaatccg cgccgctgcc tttttcgctt tcggcgttgc agattgaagc ggctaaacgt
    20581 tttggtctga gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag
    20641 ctaatcactt atccgcgttc cgattgccgc tatttgccag aagaacattt tgccggacgc
    20701 cacgcggtga tgaatgctat cagcgttcat gcaccggatc tgttgccgca gccagtggta
    20761 gatccagata tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc
    20821 attccgaccg cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac
    20881 ctgattgccc gtcagtattt gatgcagttc tgcccggatg cggtgttccg caagtgtgtt
    20941 atcgaactgg acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca
    21001 ggctggcgca cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccgctg
    21061 cctgtggtgg caaaaggcga tgaattgttg tgcgaaaaag gtgaagtggt agagcggcaa
    21121 acccagccgc cacgccattt taccgatgca acactgcttt cggcgatgac cgggatcgca
    21181 cgctttgtgc aggacaaaga tctgaaaaag atccttcgtg cgaccgatgg tttggggaca
    21241 gaagcaaccc gtgccgggat tattgaactg ttgttcaagc gtggtttcct gaccaaaaaa
    21301 gggcgctata tccactccac cgacgccgga aaagcgctat tccattcgct gccagaaatg
    21361 gcgacgcgac cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag
    21421 cagtgtcgct atcaggactt tatgcagccg ctggtgggga cgctttacca actgattgat
    21481 caggctcgta gcactccggt caggcagttc agaggaattg tggcatcggg tgggggggct
    21541 gttaaaaaag cacccaataa gcgtgtgaaa aaagcggcaa agcccaaaac agataatacg
    21601 gattctgcta attaattcgt tttccactct taaataaagc caatggtaga tattctgcca
    21661 ttggctttat tggttatact cgtcatgctt taagttgcat gtgctgcgtc tgcgttcgtt
    21721 cagcccagtc acttactcat gtaagctcct cgggattcac ttactttctg acttcctaca
    21781 actcgaatta tttagagtat atgatataag tacccatagt taaaattata aacgaaatgt
    21841 ctttacattc acgtaattta tgttatggat tgcatcatta taaattattt tcaaatgcag
    21901 ggattgtgaa cattattcac aaaatgaata tatataactg ctctataaaa tgatcagttc
    21961 atttatttga agaggataat tatgattatc ggtatcgacc tgggtacaac taacagtttg
    22021 gcagctgtct ggcgaaatgg acaaagtgaa ctgatcccca atgcgttagg taagttttta
    22081 acacccagcg tagtttgtgt tgacgaagat ggtatggttt tgacagggga ggcagctcgt
    22141 gacctgcaac tcatcaaacc acaaaactgc gcatcaaatt ttaaacgcat gatgggcaca
    22201 tcaaagacgt taaaactggg cggacgggaa tttcgtgcgg aagaactctc atcgttgata
    22261 ttacgtcagc taaaagaaga tgcagaaaac tatttgggcg aagaagtcac tgaagcggtg
    22321 attagcgttc ccgcatattt tggtgatatg caacgtaaag caacaaaagc cgctgcgaca
    22381 atggcaggct taaatgtcga acgttttatt aacgaaccaa ccgccgctgc actggcgtat
    22441 ggtttgcata ataaggatga tgaacatcaa tttctggtat tcgatttagg tggtggtacg
    22501 tttgatgtct ccatactggg gttgtttgac aatattatgg aagtacgtgc cagtgccggg
    22561 gataactttt taggtggtga agatattgtc gatatcctga tcgatgcata ttgctcacgt
    22621 cgggatcttc ctgaaaatat tgaatggcgt gaaccgacac tccagcggca tttacgcatc
    22681 gaggctgagc gtgtcaaacg cgtgctgagt gtgcgagatg aagccacttt cagtgtggag
    22741 attgaaggaa ggcgatatta ctggcatctg actacagaaa aatttgaatt tttattacag
    22801 acctttttcg aacgaatcca tatgccactg gagcgggcaa ttcgcgatgc caaaattaat
    22861 attagccaat tagaccaggt tgttcttgtg ggtggcacta cacgtatgcc actgattcgc
    22921 aaacttgtga cacgattatt tggtcgtata ccggcaatgc atttaaaccc ggatgaagtc
    22981 attgcccagg gcgcggctat tcaggctgca cttaaagcgc ggcacagcga attacgagat
    23041 gttgtagtga ctgatgtttg cccttatacg cttggcgttg atacctctaa atcgctgggg
    23101 catacccgcg aatcaggcta ttttgcaccc gttattgagc gtaacaggag tattccttgt
    23161 agtcgcgttc gtgcctttta cacggcacat gatcaacaaa cagaggttaa ttttaaaatc
    23221 taccagggtg aaagcaggat ggtggctgat aatattttcc ttgcggagtt gtcagtgaat
    23281 gttccgccaa aaccggccgg tgatgtgcgt attgatgttc gttttacgta cgacattaac
    23341 ggtattcttg atgtcgatat cagcgtgcca ctgacaggtg ctaaaaatag tctggttatt
    23401 gaacagaatc caggggcatt aacggcagaa caaatacagc agtcgttgtc caaattaagc
    23461 ctgttaaaaa ttcacccacg cgatgaacag ataaaccagg cctttattgc ccgtcttgat
    23521 aatctctatc aactcaccct ggcggaaacg cgagactgga taagcaattg ctcccgtcat
    23581 ttcagttatc tactcgagaa gcaggatcct gatcaacttg ctgattttcg gactaaagtt
    23641 gaacctacat tagactctct ggaaagagaa cgtctgcaat gaactgctgg gaaatattag
    23701 ctttacaacc aggtgcggaa gaacgagata ttaagcgtcg ttatgcgcaa ctggtaaaaa
    23761 attgccgccc ggaagatgat cccgcagcct ggcaacaatt gcacgatgct tatgaacagg
    23821 ctttacgtta tgacgacgaa gatcagtggc aagaagatga agaagagcaa ccgatcaata
    23881 tgtccagggg tattgttcat cctcgtcctg tgatgacatt tcgcgcactc aacacctttt
    23941 ctggtcatcc acttcctgat gtcactatta aatcagaggg ggaagtcacc tggcaagccg
    24001 tgctggatat gctctgtgag gagaatgaag ctattcccga acacgccccc atgcaacttg
    24061 ctaaggcgtt atctttcctt gagaaaaaag atctcagctc acgaatgtgc tttgaagaag
    24121 cgttattact gcatcttcat cgggtattcc ggccattatt aactctcgct gctgcacaaa
    24181 cttttagatg gcatttagtc gcaggcagtc gtcaacccgt aatccagaca gaaattaacg
    24241 aaagttgtct tctttattat cgcatcgaaa aacaaattac ccctttcttt gccagtaact
    24301 tgtctccact ggaagaaatg gaaaacggga aggaaatggc tacgatttat caatcattaa
    24361 aggataatga agagagcctg aaatggtttg atatcgcagt attaaataaa gttgctgaat
    24421 ttgaactatc agacaactat atggcattgt taatagagtg tttatcatat tccttgcgaa
    24481 aagaaaaaat gattcgcaag tggaaattaa cagaacgctt caataattta gcaatgcaac
    24541 atcatagtgg atataaaaag ctgctaaatg ccataaaatt aaaaggtctt ttttattatc
    24601 catcttctgt ataccagtcc tgcttacatg ctgcagagca aggctattcg ctagcctggt
    24661 attctcttgg caaaatgtat tacgaaggtg atgaggtttc acaggatcta aaattagcct
    24721 ttaactggtt tacccgcgcc gcgcagcata atgttatcga tgctctgtat gcgctgggta
    24781 tcatgtatag cgatggaagg ggcacggata agaatatttg tgaggcacga aaatggtttt
    24841 tacttgctgc ccaaaatggc aacacatctg cgcaatatga gcttgcccgt atctcacgct
    24901 ttgctgttga gccattacga aattatgaag aagcattgca gtggtattta tctgccgcca
    24961 cacaaggaca tgaggttgcc cagtatgagt tgggacaaat gtatatacaa gggattggcg
    25021 tggagcgcga tgaggttcag gcccatcgtt ggtttttgca atcagcagaa cagggatatt
    25081 tacatgcgca atatcatact gccaggttgt atagcggaag tgagagtatt tcacaggacc
    25141 aggaaaaagc gctgtactgg tttaccaaag cagcaaaaaa tggggcagat ggggcagggg
    25201 atgccatgta tgaattgggg aaatattatt taaccaacaa tgatgatcct gaaaataacg
    25261 cagaggctac tcaatggctt actggtgcag cgcagcgtgg tcgaatagag gcgatatttt
    25321 tgttagctga aatgtatctg tacggtacaa aagataccat aaaagatgaa aatcatgcgc
    25381 tgcactggta cgaaaaagcg gcgcgtttag ggtcgaccga ggcgcagcac cagactgcgg
    25441 caatgtatgc tcaggggacg ggcactaaaa ttgataataa acaagcatgg atgtggctaa
    25501 caattgccgg aaataacaac ccctggattg aaaaagatgc gttgcgatgc ttaatgtctg
    25561 aacaggatat tcaggatgca gaacaaacgg ctgcaaattg caaacgtctg ctaaggattt
    25621 aattaactca gggaaaatcg tctggttata aggaaaacca gacgattgta ataacgatta
    25681 aatcacgccc tggcttaaca tcgcatccgc aaccttcaca aaaccggcaa tgttcgcgcc
    25741 ctgcacgtag ttggtttgct caccttcacc accatgctca acacaggcat ggtggatatc
    25801 cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct ttccagccca ggcgcgcagc
    25861 gttttgcgcc atttccaggc ccgatgttgc tacaccgcca gcattagccg ctttacccgg
    25921 tgcaaacagt acgcctgcct gctggaacag ttcagtcgct tcgatggtgg tcggcatatt
    25981 tgccccttcg gcgacggctt taacgccatt ggcgataagc tgatgcgcgg cgtcaacatc
    26041 cagttcgttc tgggtggcgc aaggcagggc gatatcaacc ggtacagacc acggctgttg
    26101 gccttcgaga tagaccagac caaattcttt ggcgtaatct gccactcgac catcgcggct
    26161 ggctttgatt tcgataagac gcgccagttt ttctttcgtg aatccgcttt catcaactac
    26221 agtgccgctg gagtctgacg cagtgatcac tcgagcacca aattccatcg ctttttcgat
    26281 agcgtactgg gcgacgttgc cggagccaga aacggaaacg cgcattcctt caaaacccat
    26341 accgtggcgt tttagcattg cttctgtgaa ataaaccaga ccgtagccgg tagcttccgg
    26401 gcgaataaga ctgccgccaa atgaaaggcc cttaccggtg aagacgcagg cggtattgtt
    26461 ggagagcttt ttcatcatcc ccgccataaa gccgacttca cgaccaccaa cgccgatatc
    26521 acctgccgga acgtcagtat ccgcgcccag gtggcgatac agttcagtca tcagcgcctg
    26581 gcagaaacgc ataacttcgc cttcgctttt tcctttcggg tcgaaatcgc tgccgccttt
    26641 accaccgccc atcggtagag tagtcagggc atttttgaag gtttgttcga agccgaggaa
    26701 tttgagaatg gagaggttaa ctgacggatg gaagcgcata ccgcctttgt acgggccgat
    26761 ggcagagctg aactgcacac gccatgcacg gttgacctgc acctggttgc gatcatcaac
    26821 ccatactacg cgaaattgga tcacgcgctc cggttcaacc agacgctcca gtaatgacat
    26881 ctgacgatat ttgggatttt gttcaagaaa aggccagagt gtggtcatta cttcacgaac
    26941 ggcttgcgcg aactcggttt gattcgggtc gcgcttttgg acatggttga ggaatgactc
    27001 cagagaatat g
//
LOCUS       DAZFRE010000073        26562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFRE010000073 DAZFRE010000000
VERSION     DAZFRE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.073"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            154..912
                     /gene="ydiY"
                     /locus_tag="ACHKUI_003341"
     CDS             154..912
                     /gene="ydiY"
                     /locus_tag="ACHKUI_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6599488.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(965..1072)
                     /gene="yniD"
                     /locus_tag="ACHKUI_003342"
     CDS             complement(965..1072)
                     /gene="yniD"
                     /locus_tag="ACHKUI_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO6599489.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1243..2272)
                     /locus_tag="ACHKUI_003343"
                     /pseudo
     CDS             complement(1243..2272)
                     /locus_tag="ACHKUI_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2795..4723
                     /gene="thrS"
                     /locus_tag="ACHKUI_003344"
     CDS             2795..4723
                     /gene="thrS"
                     /locus_tag="ACHKUI_003344"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6599490.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4727..5269
                     /gene="infC"
                     /locus_tag="ACHKUI_003345"
     CDS             4727..5269
                     /gene="infC"
                     /locus_tag="ACHKUI_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6599491.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5366..5563
                     /gene="rpmI"
                     /locus_tag="ACHKUI_003346"
     CDS             5366..5563
                     /gene="rpmI"
                     /locus_tag="ACHKUI_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6599492.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5616..5972
                     /gene="rplT"
                     /locus_tag="ACHKUI_003347"
     CDS             5616..5972
                     /gene="rplT"
                     /locus_tag="ACHKUI_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6599493.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6089..6213
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6095..6139
                     /gene="pheM"
                     /locus_tag="ACHKUI_003348"
     CDS             6095..6139
                     /gene="pheM"
                     /locus_tag="ACHKUI_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6599494.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6423..7406
                     /gene="pheS"
                     /locus_tag="ACHKUI_003349"
     CDS             6423..7406
                     /gene="pheS"
                     /locus_tag="ACHKUI_003349"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6599495.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAKQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7421..9808
                     /gene="pheT"
                     /locus_tag="ACHKUI_003350"
     CDS             7421..9808
                     /gene="pheT"
                     /locus_tag="ACHKUI_003350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6599496.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLDADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9813..10112
                     /gene="ihfA"
                     /locus_tag="ACHKUI_003351"
     CDS             9813..10112
                     /gene="ihfA"
                     /locus_tag="ACHKUI_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6599497.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            10213..11193
                     /gene="btuC"
                     /locus_tag="ACHKUI_003352"
     CDS             10213..11193
                     /gene="btuC"
                     /locus_tag="ACHKUI_003352"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6599498.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            11255..11806
                     /gene="btuE"
                     /locus_tag="ACHKUI_003353"
     CDS             11255..11806
                     /gene="btuE"
                     /locus_tag="ACHKUI_003353"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6599499.1"
                     /translation="MQDSILTTVVKDIDGKVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11806..12555
                     /gene="btuD"
                     /locus_tag="ACHKUI_003354"
     CDS             11806..12555
                     /gene="btuD"
                     /locus_tag="ACHKUI_003354"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6599500.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12633..13097
                     /gene="nlpC"
                     /locus_tag="ACHKUI_003355"
     CDS             12633..13097
                     /gene="nlpC"
                     /locus_tag="ACHKUI_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6599501.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            13344..14057
                     /gene="ydiV"
                     /locus_tag="ACHKUI_003356"
     CDS             13344..14057
                     /gene="ydiV"
                     /locus_tag="ACHKUI_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6599502.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPHVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14120..15556
                     /gene="selO"
                     /locus_tag="ACHKUI_003357"
     CDS             14120..15556
                     /gene="selO"
                     /locus_tag="ACHKUI_003357"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6599503.1"
                     /translation="MTLSFITRWCDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGIWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGVNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKDDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15560..15751)
                     /gene="hemP"
                     /locus_tag="ACHKUI_003358"
     CDS             complement(15560..15751)
                     /gene="hemP"
                     /locus_tag="ACHKUI_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6599504.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15883..16929)
                     /gene="aroH"
                     /locus_tag="ACHKUI_003359"
     CDS             complement(15883..16929)
                     /gene="aroH"
                     /locus_tag="ACHKUI_003359"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6599505.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17086..17919)
                     /gene="ppsR"
                     /locus_tag="ACHKUI_003360"
     CDS             complement(17086..17919)
                     /gene="ppsR"
                     /locus_tag="ACHKUI_003360"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6599506.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            18252..20630
                     /gene="ppsA"
                     /locus_tag="ACHKUI_003361"
     CDS             18252..20630
                     /gene="ppsA"
                     /locus_tag="ACHKUI_003361"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6599507.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPLEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLSLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20687..22333)
                     /gene="fadK"
                     /locus_tag="ACHKUI_003362"
     CDS             complement(20687..22333)
                     /gene="fadK"
                     /locus_tag="ACHKUI_003362"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6599508.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEDGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(22390..22683)
                     /gene="ydiT"
                     /locus_tag="ACHKUI_003363"
     CDS             complement(22390..22683)
                     /gene="ydiT"
                     /locus_tag="ACHKUI_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6599509.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(22680..23969)
                     /gene="ydiS"
                     /locus_tag="ACHKUI_003364"
     CDS             complement(22680..23969)
                     /gene="ydiS"
                     /locus_tag="ACHKUI_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6599510.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKLPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24024..24962)
                     /gene="ydiR"
                     /locus_tag="ACHKUI_003365"
     CDS             complement(24024..24962)
                     /gene="ydiR"
                     /locus_tag="ACHKUI_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6599511.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(24982..25746)
                     /gene="ydiQ"
                     /locus_tag="ACHKUI_003366"
     CDS             complement(24982..25746)
                     /gene="ydiQ"
                     /locus_tag="ACHKUI_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6599512.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGELLQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTKRKHIILDNDSPEAIAELAEHLKKALN"
     gene            26062..>26562
                     /locus_tag="ACHKUI_003367"
     CDS             26062..>26562
                     /locus_tag="ACHKUI_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6599513.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         6608 a         6340 c         6921 g         6693 t
ORIGIN      
        1 catgcttatt caaaaaatga aataagaata cattgctaag ttattcgcgc ctttttataa
       61 ttcgttacct ttccagggac gctaatcttt gatccttaag aaagtttccg ggaccgcgat
      121 ggtctctttt tttgttgtac ggactcttga taaatgaagc ttttgaagac agttcctgcc
      181 atcgttatgt tggcgggggg catgtttgcg tcactgaatg cagctgccga tgattccgtt
      241 tttactgtca tggacgatcc tgccagcgct aaaaaacctt tcgaaggtaa tctgaacgca
      301 ggatatcttg cacaatcagg caatacgaaa agctcctcac ttacggctga caccactatg
      361 acctggtatg gacaaaccac tgcctggtcg ctgtggggga acgccagtaa tacttcttct
      421 aatgatgagc gttcttcaga aaaatatgcg gcgggcgggc gtagccgttt taatttaact
      481 gattatgact atttatttgg tcaggcaagc tggctgacag accgttataa cggctatcga
      541 gagcgcgatg tgttgaccgc gggttatggt cgccagtttt tgaatggccc ggtacacagt
      601 ttccgctttg aattcggccc tggtgtgcgt tacgacaaat ataccgacaa tgccagtgaa
      661 acccagccgc tgggctatgc ctccggggct tatgcatggc agttgactga caacgctaaa
      721 tttacgcagg gtgtttcggt ctttggcgcg gaagacacaa cgctgaactc cgaaagcgca
      781 ttaaatgttg ctattaatga acattttgga ctgaaggtgg cttacaacgt tacatggaac
      841 tctgaacctc cagagtctgc accggaacat accgatcgtc ggacaacctt atcgttgggt
      901 tacagcatgt aaaattatct gccggaccgt gttatgcggt ctggctgttt tcatttgtca
      961 ctgctcagcc ccaaatcatt gcagcccaac gtagcaacaa catagcgcca cagattgcca
     1021 gtagcaccac caccatcttc cagtgttttt tatcaaagcg tttagtcggc ataccagtgt
     1081 cctgcatatc tcttgtgcgt gataacggag tgtaacaaaa gggacaggca tcaagggatt
     1141 ttttgttatt acagtcgtcg ggtggcgttg tacacctggt cactgtttct gcattttatg
     1201 aaaaaaatta tgtatcgata tacaaatatc ataaatagcg ggttataaat acgtctttag
     1261 ttcatcagca ctaaaactca ggctttggct gattacgttt atagcagcat aatatgtttc
     1321 tacagtttta tgatgattat gatgaatggc tatatgaaca gctgacatgt tgtcatgatt
     1381 tttagcggcc aacagtgtaa ataactgacg ttggctaaaa gaatattttt ttcaaaagta
     1441 cccaacgttg atatataaga taacacgaca tctttactta ctgacaaagt atgattgtct
     1501 atatataaaa tcgcggacat atgactgaca ttgtttgcca tcattcgaca gaagaaaata
     1561 tctaaagcaa agccaaaatc ctgactcgcg ataacgcgac tccttgcaag aggatggcga
     1621 agtaatgctc tgaattccgc gtcccggact aagggaacat gctgctgtat ggattcaaca
     1681 gaggagtaat gtttaatcag gttgactttg ccttttccat ttcgcaatga atcaagggcg
     1741 tattgtaatg acggatggcg acaaaaaata gtgtcagaag gagattgcga aaaaatgcac
     1801 tatcattgag atttactgtt ggctgtatgg ttttttctct atgaattttt ttggacagtt
     1861 tcatgcaatg gttacgtctt tcggcagtta actgttcctg ttcaatataa tccagtatgc
     1921 ttcgcaaatg gttagcttta ataggcgtgg tttccaggaa taaggaaatg atttcttttt
     1981 tatagtgtaa tgtaatgagt gtattttctg catggatggg tgaagtggca aagttaatga
     2041 taaaaagggc agtggaataa caattttgtg aaatttttcg acagcttgcg gtaaattcct
     2101 gagtaacaca gtatgacagc atcgaactta aataagaggt atcaagtagg gtgatgagct
     2161 ggcgcgcaaa ttgattaata ggctctgatg aaggcgttag tatgtcgcta tcgatgtgca
     2221 ttctaagagg agacttataa tgagttccga taatgatatc gtttttactc ataatctatt
     2281 cgccttatat atgtctggag attttgagga tgatgattga ttattatcgc cagaacgaaa
     2341 tgcgcacgaa aaattgctat aagaattatg tcacttttat cattgcgata tgactacttc
     2401 gtcattaatc tcatgatcgt ttaaatacta ccgccaaatc acgcaacgaa ctttgctgga
     2461 ttcagtttat gctgctgtaa atccgctcgt agtaaacctt tcagacactc tgtgatgtta
     2521 tcagtggttc tttaagcgtt ttgctggtgt actcactaca aacgaattgc gaatcgaatc
     2581 aatgtgaaac ggaaaggtac aatctccctt tttttagtgt taacatcgtt caaccgggtg
     2641 gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat ttagcatttg
     2701 ttggctagat ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt atgtgatctt
     2761 tcgtgtgggt caccactgca aataaggata taaaatgcct gttataactc ttcctgatgg
     2821 cagccaacgc cattacgacc acgctgtaag ccccatggat gttgcgctgg acattggtcc
     2881 aggtctggcg aaagcctgta tcgcagggcg cgttaatggc gaactggttg atgcttgcga
     2941 tctgattgaa aacgacgcac aactgtcgat cattaccgcc aaagacgaag aaggtctgga
     3001 gatcattcgt cactcctgtg cgcacctgtt agggcacgcg attaaacaac tttggccgca
     3061 taccaaaatg gcaatcggcc cggttattga caacggtttt tattacgacg ttgatcttga
     3121 ccgcacgtta acccaggaag atgtcgaagc actcgagaag cggatgcatg agcttgctga
     3181 gaaaaactac gacgtcatta agaagaaagt cagctggcat gaagcgcgtg aaactttcgc
     3241 caaccgtggg gagagctaca aagtctccat tcttgacgaa aacatcgccc atgatgacaa
     3301 gccaggtctg tacttccatg aagaatatgt cgatatgtgc cgcggtccgc acgtaccgaa
     3361 catgcgtttc tgccatcatt tcaaactaat gaaaacggca ggggcttact ggcgtggcga
     3421 cagcaataac aaaatgttgc aacgtattta cggtacggcg tgggcagaca aaaaagcgct
     3481 taacgcttac ctgcaacgcc tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa
     3541 acagctcgac ctgtaccata tgcaggaaga agcgccgggt atggtattct ggcacaacga
     3601 cggctggacc atcttccgtg aactggaagt gtttgttcgt tctaaactga aagagtacca
     3661 gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca
     3721 ctgggacaac tacaaagatg caatgttcac cacgtcttct gagaaccgtg aatactgcat
     3781 taagccgatg aactgcccgg ggcacgtaca aattttcaac caggggctga agtcttatcg
     3841 cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac cgtaacgagc cgtcaggttc
     3901 gctgcatggc ctgatgcgcg tgcgtggttt tacccaggat gacgcgcata ttttctgtac
     3961 tgaagaacaa attcgcgatg aagttaacgg atgtatccgt ttagtctatg atatgtacag
     4021 cacttttggc ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg
     4081 cagcgacgaa atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa
     4141 catcccgttt gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac
     4201 cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg acttctcttt
     4261 gccttctcgt ctgagcgctt cttatgtagg cgaagacaat gaacgtaaag taccggtaat
     4321 gattcaccgc gcaattctgg ggtcgatgga acgtttcatc ggtatcctga ccgaagaatt
     4381 tgctggtttc ttcccgactt ggcttgcgcc agttcaggtt gttatcatga atattaccga
     4441 ttctcagtct gaatacgtta acgaactgac gcaaaaacta tcaaatgcgg gcattcgtgt
     4501 taaagcagac ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg
     4561 cgtcccatat atgctggtct gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg
     4621 cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg agaagctgca
     4681 acaagagatt cgcagccgca gtcttaaaca attggaggaa taaggtatta aaggcggaaa
     4741 acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc aggaagttcg
     4801 cttaacaggt ctggaaggcg agcagcttgg tattgtgagt ctgagagaag ctctggagaa
     4861 agcagaagaa gccggagtag acttagtcga gatcagccct aacgccgagc cgccggtttg
     4921 tcgtataatg gattacggca aattcctcta tgaaaagagc aagtcttcta aggaacagaa
     4981 gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta cagatgaagg
     5041 cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg ataaagccaa
     5101 aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta tggaagtgct
     5161 taatcgcgtg aaagacgatt tgcaagaact ggcagtggtc gaatccttcc caacgaagat
     5221 cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagtaag gccttcaagt
     5281 agcactgtct gtggagcctt tgggcttcac aggcattgtt cgcctttgtt tcgtttatta
     5341 acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa
     5401 gcgcttcaaa aaaaccggta aaggtggttt taagcacaag cacgctaacc tgcgtcacat
     5461 tctgaccaaa aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa
     5521 aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta acgtttttaa
     5581 ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta
     5641 ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac ggtgcgcgtt
     5701 ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat gcttaccgcg
     5761 accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc aacgcagcag
     5821 cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg
     5881 aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg ttcaccgctc
     5941 tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag ctagtctccc
     6001 tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt ttcaataaag
     6061 gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat gctgctattt tccgcttctt
     6121 tttttacttt agcacctgac tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc
     6181 gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcct
     6241 gtctcatcag gcgtaattgt cgcagccagt ctggacacgg ccagcgcgga gaaaccggag
     6301 cgtacttaag tacgtgagga tttcgagcac tgcccgggac caaaatggca agtaaaatag
     6361 cctgatggga tgggctctaa gtccaacgaa ccagtgtcac cactgacaca atgaggaaaa
     6421 acatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc caggcgtcag
     6481 atgttgccgc gttagataat gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc
     6541 ttcagatgac gaccctgcgt gagctgccgc cagaagagcg tccggcagct ggtgcggtta
     6601 tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa
     6661 gcgccgcact gaatgcgcgt ctggcggcgg aaacgattga tgtctccctg ccgggtcgtc
     6721 gcattgaaaa cggtgggctg catccggtga cccgtaccat cgaccgtatc gaaagtttct
     6781 tcggtgagct tggctttacc gtggcaaccg ggccggaaat cgaagacgat tatcataact
     6841 tcgatgctct gaacattcct ggtcaccacc cggcgcgcgc tgaccacgac actttctggt
     6901 ttgacgctac ccgcctgctg cgtacccaga cctctggcgt acagatccgc accatgaaag
     6961 ccaagcagcc accgattcgt atcatcgcgc ctggccgcgt ttatcgtaac gactacgacc
     7021 agactcacac gccgatgttc catcagatgg aaggtctgat tgttgatacc aacatcagct
     7081 ttaccaacct gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag gaagatttgc
     7141 agatccgctt ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa gtggacgtca
     7201 tggggaaaaa cggtaaatgg ctggaagtac tgggctgcgg gatggtgcat ccgaacgtgc
     7261 tgcgtaacgt tggcatcgac ccggaagttt actctggttt tgccttcggg atggggatgg
     7321 agcgtctgac catgttgcgt tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc
     7381 tgcgtttcct caaacagttt aaataaggca ggaatagatt atgaaattca gtgaactgtg
     7441 gttacgcgaa tgggtgaacc cggcgattga tagcgacgcg ctggcaaatc aaatcacgat
     7501 ggcgggcctg gaagttgatg gtgtagaacc ggttgccggt agcttccacg gcgtggtcgt
     7561 tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt
     7621 gaatgtcggc ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact gccgtcaggg
     7681 cctgcgtgtg gcggtagcga ccattggtgc tgttctgccg ggtgatttca aaattaaagc
     7741 cgcgaaactg cgtggcgaac cgtctgaagg gatgctgtgc tccttctctg agctgggaat
     7801 ttctgacgat cataacggca ttatcgagct gcctgcggat gcgccgattg gcaccgacat
     7861 ccgcgaatac ctgaaactcg atgacaacac catcgaaatt agcgtgacgc caaaccgtgc
     7921 cgactgctta ggtatcattg gtgttgcgcg cgacgttgcc gtgttgaacc agctgccgct
     7981 ggttgaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgctgc cgattgcagt
     8041 agaagcgccg gatgcctgcc cgcgctatct tggccgtgtg gtaaaaggca ttaacgttaa
     8101 agcgccaact ccgctgtgga tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga
     8161 tgcagttgtt gacgtcacca actatgtgct gctcgaactg ggccagccaa tgcacgcttt
     8221 cgataaagat cgcattgaag gcggcattgt ggtgcggatg gcgaaagagg gcgaaacgct
     8281 ggtgttgctc gacggcactg aagcgaagct ggatgctgac accctggtca tcgccgacca
     8341 caacaaggcg ctggcgatgg gcggcatatt tggtggcgaa cactctggcg tgaatgacga
     8401 aacgcaaaac gttctgctgg aatgcgcttt cttcagcccg ctgtctatca ccggtcgtgc
     8461 tcgtcgtcat ggcctgcata ctgatgcgtc tcaccgttat gagcgtggcg ttgatccggc
     8521 actgcagcac aaagcgatgg aacgtgcgac ccgtctgctg atcgacatct gcggtggtga
     8581 agcgggtccg gtaattgata tcactaacga agcaacgctg ccgaagcgtg caaccatcac
     8641 tttacgtcgt agcaaactgg atcgcctgat cggccatcat attgctgatg agcaggtaac
     8701 agacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgagt ggcaggcagt
     8761 tgcgccgagc tggcgtttcg acatggagat tgaagaagat ctggtcgaag aagtcgcgcg
     8821 tgtttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga ttatggggac
     8881 tcaccgtgaa gctgacctgt cgctcaagcg cgtaaaaacg ctgctcaacg acaaaggcta
     8941 tcaggaagtg atcacctaca gcttcgttga cccgaaagtg cagcagatga tccatccagg
     9001 cgttgaagcc ttactgctgc caagcccgat ctctgttgaa atgtcagcaa tgcgtctttc
     9061 cctgtggacc ggcctgctgg caaccgtggt gtacaaccag aaccgtcagc agaatcgtgt
     9121 acgcattttc gaaagcggtc tgcgtttcgt gccagatact caggcaccgt tgggtattcg
     9181 tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc actggaacct
     9241 ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg ttctcgacct
     9301 gaccggtaaa ctgaatgagg ttgagttccg tgcagaagcg aatccggcac tgcatccggg
     9361 gcaatccgca gcgatttatc tgaaaggtga acgtattggt tttgttgggg ttgttcatcc
     9421 tgaactggaa cgtaaactgg atctcaacgg tcgcactctg gtgttcgaac tggagtggaa
     9481 caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt tctcgcttcc cggcgaaccg
     9541 tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt tatccgaatg
     9601 taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt accgcggtaa
     9661 gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag ataccagccg
     9721 tacactcgaa gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga
     9781 gcgattccag gcatcattga gggattgaac ctatggcgct tacaaaagct gaaatgtcag
     9841 aatatctgtt tgataagctt gggcttagca agcgggatgc caaagaactg gttgaactgt
     9901 ttttcgaaga gatccgtcgc gctctggaaa acggcgaaca ggtgaaactc tctggttttg
     9961 gtaacttcga tctgcgtgat aagaatcaac gcccgggacg taacccgaaa acgggcgagg
    10021 atattcccat tacagcacgg cgcgtggtga ccttcagacc cgggcagaag ttaaaaagcc
    10081 gggtcgaaaa cgcttcgccc aaagacgagt aatctgatct aactaaaaag gccgctctgc
    10141 ggcctttttt cttttcactg tcgaagagtc accgtaaaat caacgccatg acacttcagc
    10201 agaacggata ccatgctgac acttgcccgc caacaacagc gacaaaatat tcgctggtta
    10261 ttatgcttgt cagttttgat gctactggcg cttctcttaa gcctttgcgc cggtgaacta
    10321 tggattttgc caggtgactg gttttctcct cgtggcgaac tgttcgtctg gcaaattcgc
    10381 ctgccacgta cgctggctgt attgctggtt ggtgcggcgc tggctatatc cggcgctgta
    10441 atgcaggcgt tgtttgaaaa tcctctggca gaacctggac tacttggcgt ctctaacggc
    10501 gcaggcgtgg ggcttatcgc cgcggtatta cttgggcaag ggcaactccc caactgggcg
    10561 ctagggctgt gtgcgattgc tggcgcgctt atcatcactt taatactctt acgtttcgcc
    10621 cgtcgtcatc tttcgaccag tcggttattg ctggctggcg ttgcattagg gattatctgt
    10681 agcgcactaa tgacgtgggc tatctacttt tccacctctg ttgatttacg tcagctgatg
    10741 tactggatga tgggcggttt tggcggcgta gactggcggc aaagctggct gatgctggca
    10801 ttgatcccgg tgttgttgtg gatctgttgt cagtccaggc cgatgaatat gttagcactt
    10861 ggcgagatct cggcgcggca actgggttta cccctgtggt tctggcgcaa tgtgctggtg
    10921 gcagcgaccg gctggatggt tggcgtcagt gtggcgctgg cgggtgctat cggctttatt
    10981 ggtctggtga tcccacatat tctccggctg tgtggtttaa ccgatcatcg cgtattactt
    11041 cccggctgcg cgctggcagg ggcgagcgca ttgctgctgg ccgatattgt agcgcgcctg
    11101 gcattagctg ccgcagagct gccaattggc gtggtcaccg caacgttggg tgcgccggtg
    11161 tttatctggt tattgttaaa agcaggacgt tagccgcaaa acacggtcta tgattaaaag
    11221 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
    11281 agtgaaagat attgacggta aagtgaccac gctggagaag tacgccggta atgtgctgtt
    11341 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
    11401 tcagaaagcc tgggctgatc gtggttttgt ggtgctggga ttcccgtgta accagtttct
    11461 ggagcaagaa ccgggtagcg atgaagagat taaaacttat tgcaccacca catggggggt
    11521 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgtcatc cgctgtatca
    11581 aaaattgatt gccgcagcgc cgactgcagt cgcgccggaa gagagcggat tctatgcccg
    11641 aatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga attttgagaa
    11701 atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata tgacgccgga
    11761 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
    11821 cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga ggttcgggct
    11881 ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt actggcgcga
    11941 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
    12001 tggtctgcaa caaagctcgc gctgcatcgc gcctatcttt cacaacagca gacgccgccg
    12061 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
    12121 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
    12181 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgct gcaaatcaca
    12241 ccacaagcca atcccgcagg ccaattgctg cttcttgatg agccgatgaa cagtcttgat
    12301 gttgcgcaac aaagtgcctt agacaaaatt ctgagcgcgc tgtgtcagca aggactggcg
    12361 attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg ggcgtggttg
    12421 ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag aggtgctcac gccggcaaat
    12481 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    12541 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    12601 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    12661 cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg
    12721 attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    12781 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtgacga
    12841 tgcgcgatcg tttcgatttg cagctgcccc gtgaaaccaa acaacaagcc tctattggca
    12901 cgcaaattga taaagacgag ttgctgccag gtgacctggt ctttttcaaa acgggttccg
    12961 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    13021 ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc
    13081 aggcgagacg aatctagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta
    13141 ctcctgaata cagtagtatt ttgcagacaa aaattccgta aatggattct tcgtttataa
    13201 tcaggataat ctaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt
    13261 actccttctg taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    13321 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    13381 gctatttcct gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc
    13441 atttctccag tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta
    13501 ccgaggagca acattggcaa ttattttctg aacagttgga attactaaaa tcatgccaac
    13561 acttttttat tcaacataaa ctctttgcct ggcttaattt aacgccacac gtggctacat
    13621 tattattaga cagggataat ttcgcaggcg aactattaaa atatccgttt attgaactgt
    13681 tgattaatga gaattacccg catttcaatg aaggaaaaga taatcgcgat cttttatcct
    13741 tatcgcaaat gtatcccctt gtgttaggta atttgggagc aggtaatagc acaatgaaag
    13801 cggtttttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    13861 tcacgcatcg ttccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt
    13921 gcaattgcat catcgcgggg ggcattgata cgccagaaat attggcacag attatcccct
    13981 ttgatttcca tgctctccag ggatgcctgt ggcctgcggt tcctataaat cagataacga
    14041 cgttggttca gcgataaccc tcctgtttgc tggtgtttag gacgagagta accgtctaca
    14101 ctatcaaaca ggaggatcta tgaccctgtc ttttattacc cgctggtgcg atgaattgcc
    14161 agaaacctat acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    14221 taccgaactc gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt
    14281 ctggggcggc gaaaccctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    14341 tcagttcggc atctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca
    14401 actgcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    14461 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    14521 tgaggcgatg cattatctgg gcattccgac gacccgcgcg ttaagtatcg tcaccagcga
    14581 ttcgccagtg tatcgggaaa cggtggagtc aggcgcgatg ctgatgcgtg tggcaccaag
    14641 tcatctgcgc tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg
    14701 tcagttagct gactttgcca ttcgtcatta ctggtcacat cttgatgatg aggaggacaa
    14761 ataccgtctc tggtttaccg atgttgtcgc acgtaccgcc tcgttaattg cccaatggca
    14821 gacggtcggc tttgctcatg gagtgatgaa taccgacaac atgtcgctgc tggggctgac
    14881 gcttgattac gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca
    14941 ctcggatcat caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt
    15001 acagcgtctg gcgcagacat tgtcgccatt tgttgccgta gatggcgtga atgaggccct
    15061 ggacagctat cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg
    15121 cttcatgacg gagcaaaaag aggataacgc gctactgaat gaattattca gtctgatggc
    15181 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag
    15241 cgcggcgtca ccgctacgtg atgagtttat tgatcgtgcg gcatttgatg actggtttgc
    15301 ccgttatcgg gggcgtttgc aacaagacga gattaccgat agtgagcgtc agcaactgat
    15361 gcaaagcgtt aacccggctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    15421 ggcagaaaag gatgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    15481 cagcgacaga gatgatgact atgtcagtcg tccacccgac tggggtaaac ggctggaagt
    15541 cagctgctcg agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga
    15601 gcagatattc ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    15661 ggctggtaat tcgccggatg ggctgcgggg aagcggtctt gtgactggca tccgtttcag
    15721 gcgtgagttt tctgctatcc gtataacgca taaaaattaa taataactta tataacaatg
    15781 attcttatta tcaatattat ggcgggccag agcaagcagt aatttgttga gaagacaaca
    15841 agttgcgcag aaatgcccat cccgtcagga atgggcacgc attcagaagc gagtatctac
    15901 cgcagaggcg agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt
    15961 aatggattga ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag
    16021 gaaactttcc gccataattc cagcaatcgc cgtagagcca ttgcggattt gctgacaaat
    16081 atcctcacaa acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc
    16141 caccaccaga tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc
    16201 atcggcatga taattcggtt ttttgccacc acgcataata atgtggccat acgggttgcc
    16261 gctggtctga tagatggtca tctgaccatt tttgtctggc gagaggaaca tatggctggc
    16321 gcgggctgcg cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa
    16381 acccaccgga caggagagtg ccgaagccat ttcgcggtgg atctgacttt cggtagtacg
    16441 tgcgccaatc gcaccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag
    16501 gaactcagtc gcggtaggga cgcccagctc atttacctgt aaaagtaatt tgcgcgccag
    16561 ctccagaccg tgatttaccc gatagctacc gtttaaatct ggatcggaga tgagtccttt
    16621 ccagccgaca accgttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtga
    16681 ctggtactgg ttgcgcagcg actgcagacg ggtggcgtac tccatcgcag cggtgagatc
    16741 gtggatcgag caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt
    16801 ttcaattcta cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg
    16861 tagcgcgagt tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt
    16921 tctgttcatt acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat
    16981 gcgcatcacg ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg
    17041 ggtggttagc atgataacaa aaaaacaagt taatgcacta gttctctagt acattcggcg
    17101 gctaaggccc atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact
    17161 gttaatccac gggatctgat ttttacggta caacgcttcc acttccgcga cttccatcct
    17221 gcactgacga agcgaggcat agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag
    17281 acgttccggg tcgatagtca ggccgaacaa tttgtgctga agtggtttga gcgacgcggg
    17341 tagcaccaga ttatccatat cgtcggcaat aaaggggtaa tttgccgcgc ggataccaaa
    17401 ttgcattgcc agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat
    17461 cacctgagcc tggtccagat tgcgcaacga aataccgtca tcgtgggcaa gggtgtaatc
    17521 aatcgccgca atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg
    17581 atgagcaatc ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg
    17641 aacgatatcc tggcaaaagc cttcactttg caagatgata gcgcgaatct ccggcaacac
    17701 gatggagtag aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc
    17761 cttcactgcc cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt
    17821 gacgggaaat tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc
    17881 agaaatataa aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag
    17941 acataattct atgaaagcat aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt
    18001 ttataaaatg aaacttaaat tttatttttt atgaaaacag catttcattt ttatggtttc
    18061 gtttataccg atggtttatg tggaaattgt cgaagagagc agatttgcgc aacgctggga
    18121 tcagtcttaa aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg
    18181 ttaaatatgc aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa
    18241 aggattgttc gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg
    18301 gcatgaatga tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta
    18361 acctttccgg aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta
    18421 accagtttct ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg
    18481 atattgacga tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg
    18541 acactccctt ccagcctgag ctggaaaacg ccatccgcga agcctatgca cagctttccg
    18601 ccgatgacga aaacgcttcg tttgcggtgc gttcctccgc tactgcagaa gatatgccgg
    18661 acgcttcttt tgccggtcag caggaaactt tcctcaacgt tcagggtttt gacgccgttc
    18721 tcgtggcagt gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg
    18781 tgcaccaggg ttacgatcac cgtggcgtgg cgctctccgc cggtgttcag cggatggtgc
    18841 gctccgacct cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc
    18901 aggtggtgtt tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggtgcggtta
    18961 acccggatga gttttatgtg cataaaccga cacttgcggc gaatcgcccg gctatcgtgc
    19021 gccgcaccat ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca
    19081 agcaggttaa aatcgaagac gtaccgctgg aacagcgtga catcttctcg ctgaccaacg
    19141 aagaagtgca ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga
    19201 tggatattga gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc
    19261 cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta
    19321 agattatcgc cgaaggccgt gctatcggtc atcgtatcgg cgcgggtccg gtgaaagtca
    19381 tccatgacat cagcgaaatg aaccgcatcg aacctggcga cgtgctggtt actgacatga
    19441 ccgacccgga ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg
    19501 gtcgtacctg tcacgcggcg atcatcgctc gtgaactcgg cattccggcg gtagtgggct
    19561 gtggtgatgc aacagaacgg atgaaagacg gcgagaacgt cactgtttct tgtgccgaag
    19621 gtgataccgg ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag
    19681 aaacgatgcc ggacctgtcg ttgaaggtga tgatgaacgt cggtaacccg gaccgtgctt
    19741 tcgacttcgc ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca
    19801 accgtatgat tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt
    19861 tgcaaaacga aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg
    19921 gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg
    19981 tccgtctttc tgattttaaa tcgaacgaat atgccaatct ggtcggtggt gagcgttacg
    20041 agccagatga agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca
    20101 gcttccgcga ctgcttcgcg ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg
    20161 ggctgactaa cgttgagatc atgatcccgt tcgtgcgtac cgttgatcag gcgaaagcgg
    20221 tggttgagga actggcgcgt caggggctga aacgtggcga gaacgggctg aaaatcatca
    20281 tgatgtgtga aattccgtcc aacgccttgc tggcagagca gttcctggaa tatttcgacg
    20341 gcttctcaat tggctcaaac gacatgacgc agctggcgct cggtctggat cgtgactccg
    20401 gcgtggtgtc cgaactgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg
    20461 cgattcgtgc cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccatccg
    20521 accacgaaga ctttgctgca tggttgatgg aagaggggat cgatagcctg tctctgaacc
    20581 cggacaccgt ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatacc
    20641 cggcggcgtt ttgtcgccgg gtttatgtga tccccgaaga tgaaacttat tcaatctctt
    20701 cacagacatc ctgagttaat cgccgcataa tatctttcct taacaaaaac ttttgtattt
    20761 tacctgagac agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt
    20821 ttgcgacccg tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg
    20881 gcgctttcag cacgacataa gcgcatgaac gttcacctaa acgttcatcg ggcatcgcaa
    20941 ccacacaggc atcgtgaatt ttaggatgct gcaataaaat atcttctact tcacggctgc
    21001 taatattttc gccgccgcgg acaataatat ctttcttgcg cccggttatt tttatatagc
    21061 catcctcatc catgcggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac
    21121 gggcggttaa ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt
    21181 cttcaccttc gcaacctggc ggtaaagtct tgcgtgcgtc atcgaccact ttaatctcta
    21241 cacctgcggc agcgtaaccg tcggtgtgca taaagcgcgg caaaggatcg tcgagattca
    21301 ccaccgcatg cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct
    21361 gctggcattc tcgcgccact tttttgggga ttgtggtacc tccgcaaaga aagaagcgca
    21421 gcgctgaaag gtctgcgggt tgtttctcta gtaaattcaa aagatcatag acaaacggcg
    21481 ttgcgccgag catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag
    21541 tgaaaatatc taacaacacg ctgcgagcac caatcaaaaa aggtgctgtt acgccatgca
    21601 gaaagcccgt tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat
    21661 ttagccgcgc gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacac
    21721 cctttggcag accctcggtt ccggaggtaa atagcacagc ggctaattca tcgccgtgga
    21781 ccgttatcgc cgtggtcaga ggagtattgt cggcgagaat ctgacttaat gagagggaag
    21841 aggtggcggg agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt
    21901 gcagcggcag gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca
    21961 tttttgcctg acacttattg agcacccaca ccagttctgc ttcccgccag gaaggtaaca
    22021 gcggcacgga aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc
    22081 agcctggcag ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt
    22141 ttgccagaca gctcgcggcg tgatcgagcg cgctataggt gtacgttgca ccatgattgt
    22201 cgaccacggc aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagtg
    22261 aagcatcgcc ccataaccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta
    22321 atgtcacttt cattttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg
    22381 gatgcatgat tagccgtagc gaaattcaat accgaaggtg cctgcgggat attgccactg
    22441 ttcgagaata gtgttgccgc acagcacccg acaggtgccg cactccagac aaccggcgga
    22501 atcgaaatga atgtttcctg cgtcatcctg cttataaagt ccggcagggc aagctttcat
    22561 taatttacgg aattcattga tatcgggatt tgccgccaaa atgatatgcg ggtggccctc
    22621 atcaacatgg aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct
    22681 catagcgcgg ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc
    22741 gcgtgtccca tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacata
    22801 tcgttcatga tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc
    22861 agcgccggga gcttgcggaa atgctgcata tcgcgcataa cacaactttg ttccagctca
    22921 cgtttgtatt gcgccagact gctggcggag aaatccgcgc gttctttggc ggcgatcacc
    22981 gttgtggcgg cagcctgagc tgatgcaatg gctaaatcca tgccgcgaac tgtaaaaccc
    23041 aaattcaggc agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccagctgc
    23101 ggcaccattg ccagaccgcc ctccggcacc atatgcgcgg aatattcaag cagtttgccg
    23161 ccgctaatca gcgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg
    23221 cttttttgcg catgggcgat atcacccaat ccacaaacca gccccaacga tacggaatcc
    23281 ttgttggtat agaggaatcc gccgcccatc aggccgtcag aaggggaacc ggcaaacagc
    23341 caggcggcac cttcctcgcc cgtaacatta aagcgatcgt tgatctgttc tggtgtcagg
    23401 cctataacct ctttaacacc aacagcgtaa tgatgcggat tggaagcgga aaccattccc
    23461 agcgagcggc caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga
    23521 atatcatccc cggcctggac gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg
    23581 cgtacgcccg ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc
    23641 agacgattac gcaatacggt ataagacgcg tgttgcggaa catctggttg ctcgcggtga
    23701 aaatcgaggg taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg
    23761 cgttctaccg gcgctgatgc tgcaaagcct ggaataattg cttcaagtgt gtgggcataa
    23821 agacgcccgc cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca
    23881 tccagcccgg ctcgcgccat gaccagtgcg gcaacgctac cggcaacgcc cgcaccgacc
    23941 acaatggcat caaatttgtc atccgacatg cgttatgctc ccttcccgct gcgtttacag
    24001 cgggagatgt ggttgaaagg gaattagcga ttcaactggc taatcagggc agggacgact
    24061 ttgtagatat cgcccaccag accgtagtcg gcatagttga agattggcgc atttttatct
    24121 ttattaatgg cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg
    24181 gagatcccca gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa
    24241 cgttcacgct ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca
    24301 ttcagcactg ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc cagaccacga
    24361 ccgacgccaa ccacacgttt tgctttgctc aggtccacgc tgcttgccgc tttcgcacgg
    24421 cgttcgcgac agagaatttc atgacgcggg gcaacataag gtaccgtttc tgtcgggcac
    24481 tgatgagaga tatcacgggt gcacggctcc tgaacaccgg gtgcaagggt aatgatcgcc
    24541 agcgggctgt tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg
    24601 caaatgtgac catcgacaat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt
    24661 tgcacactta accgtgcggc cagagcttta ccacgtttcg tcgcggccaa cagcaacata
    24721 gcggggtgtt tatctttcag cagggcagca atgctttcgg cgtaattttc agtacgttgc
    24781 agcgcgtcgt tttgctcaag aacataaatg cattttggcc cataaggcat aaccgcctgc
    24841 gcctgctcgg tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca
    24901 aacagttcag cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc
    24961 atcgtttctt ctccgtaggc ttcagttcag ggcttttttc agatgttccg ccagctcggc
    25021 aatggcctcc ggcgaatcgt tatcgagaat gatgtgctta cgttttgttt gcggcggtac
    25081 gcgaataccg gtcagttcag caagtggtgc gctctggctc cagccaatat cacttgcctg
    25141 ccactgattt accggttttt tacccgcgcc gaggatggct ttcatcgaag gaatacgtgg
    25201 tacgttaata tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac
    25261 atcatcttca agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa tagcactcac
    25321 tgcattaatc accggaagtt gcagaagttc tccgaccagc aagccaacct gctgggcata
    25381 aaggtcgccg gaaccttcac caaagatcag taaatcgaag ccgatctttt caactgctgt
    25441 cgccagcgcc tttgcggtat cgagaggcag tgcatgctca agttgtgcat cctgcaccat
    25501 atacaggctg tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa
    25561 agagccgccg acggtcagcg cggctatctc atcgtcatct gttgcaagct ggcttgcagc
    25621 ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt
    25681 gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat
    25741 tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc
    25801 ctgaaaatag tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc
    25861 aacaatcttg caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt
    25921 tattccatga ccgtgcaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa
    25981 cgcggttgta taaccgcaat acacgccgta ttttatccgg catatattgt ttttgtttgc
    26041 tcatggaata gggaggttgt catgtatcaa cgctgttttg ataatgccag tgaaacgctg
    26101 tttgtcgccg gtaaaacgcc acggctttca cgtttcgcat ttagcgatga tccaaaatgg
    26161 gagtctggac atcacgttca tgacaatgaa accgagctga tttacgtcaa gaaaggggta
    26221 gcaaggttta ccatcgattc ttcgttatat gtcgcgcatg ccgatgacat tgtggtgata
    26281 gaacgtggca ggttgcatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc
    26341 tgtgcgctgt acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat
    26401 tcttgcccgg taattgccgc aggacaggga aaagaagtca ttaaaacctt atttaatgag
    26461 ctaagtgtga ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggacgcattt
    26521 gcctatacgt tagcaattct ttactacgaa aactttaaaa at
//
LOCUS       DAZFRE010000074        26204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFRE010000074 DAZFRE010000000
VERSION     DAZFRE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.074"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            80..796
                     /gene="nanC"
                     /locus_tag="ACHKUI_003368"
     CDS             80..796
                     /gene="nanC"
                     /locus_tag="ACHKUI_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6599514.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            816..1922
                     /gene="nanM"
                     /locus_tag="ACHKUI_003369"
     CDS             816..1922
                     /gene="nanM"
                     /locus_tag="ACHKUI_003369"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6599515.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN"
     gene            1987..2967
                     /gene="nanS"
                     /locus_tag="ACHKUI_003370"
     CDS             1987..2967
                     /gene="nanS"
                     /locus_tag="ACHKUI_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO6599516.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGACWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3077..3282
                     /locus_tag="ACHKUI_003371"
                     /pseudo
     CDS             3077..3282
                     /locus_tag="ACHKUI_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3550..4790)
                     /gene="yjhR"
                     /locus_tag="ACHKUI_003372"
                     /pseudo
     CDS             complement(3550..4790)
                     /gene="yjhR"
                     /locus_tag="ACHKUI_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_176259710.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase YjhR"
     gene            4906..5037
                     /locus_tag="ACHKUI_003373"
     CDS             4906..5037
                     /locus_tag="ACHKUI_003373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599517.1"
                     /translation="MTQVLIGDIYLRQQCDVFWLGYTISPAYARQGYAIEAITATID"
     gene            5528..5785
                     /gene="topAI"
                     /locus_tag="ACHKUI_003374"
     CDS             5528..5785
                     /gene="topAI"
                     /locus_tag="ACHKUI_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HFO6599518.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            5797..6342
                     /gene="yjhQ"
                     /locus_tag="ACHKUI_003375"
     CDS             5797..6342
                     /gene="yjhQ"
                     /locus_tag="ACHKUI_003375"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6599519.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            6398..7144
                     /gene="yjhP"
                     /locus_tag="ACHKUI_003376"
     CDS             6398..7144
                     /gene="yjhP"
                     /locus_tag="ACHKUI_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6599520.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            7313..7531
                     /locus_tag="ACHKUI_003377"
                     /pseudo
     CDS             7313..7531
                     /locus_tag="ACHKUI_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7569..7685
                     /locus_tag="ACHKUI_003378"
                     /pseudo
     CDS             7569..7685
                     /locus_tag="ACHKUI_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            7930..9051
                     /gene="sgcX"
                     /locus_tag="ACHKUI_003379"
     CDS             7930..9051
                     /gene="sgcX"
                     /locus_tag="ACHKUI_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFO6599521.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            9048..9326
                     /gene="sgcB"
                     /locus_tag="ACHKUI_003380"
     CDS             9048..9326
                     /gene="sgcB"
                     /locus_tag="ACHKUI_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB SgcB"
                     /protein_id="HFO6599522.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            9338..10651
                     /gene="sgcC"
                     /locus_tag="ACHKUI_003381"
     CDS             9338..10651
                     /gene="sgcC"
                     /locus_tag="ACHKUI_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC SgcC"
                     /protein_id="HFO6599523.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            10664..11470
                     /gene="sgcQ"
                     /locus_tag="ACHKUI_003382"
     CDS             10664..11470
                     /gene="sgcQ"
                     /locus_tag="ACHKUI_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HFO6599524.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            11601..12032
                     /gene="sgcA"
                     /locus_tag="ACHKUI_003383"
     CDS             11601..12032
                     /gene="sgcA"
                     /locus_tag="ACHKUI_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcA family PTS sugar transporter subunit IIA"
                     /protein_id="HFO6599525.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            12044..12676
                     /gene="sgcE"
                     /locus_tag="ACHKUI_003384"
     CDS             12044..12676
                     /gene="sgcE"
                     /locus_tag="ACHKUI_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HFO6599526.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            12693..13475
                     /gene="sgcR"
                     /locus_tag="ACHKUI_003385"
     CDS             12693..13475
                     /gene="sgcR"
                     /locus_tag="ACHKUI_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6599527.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            13778..14566
                     /gene="yjhI"
                     /locus_tag="ACHKUI_003386"
     CDS             13778..14566
                     /gene="yjhI"
                     /locus_tag="ACHKUI_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6599528.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            14571..15476
                     /gene="yjhH"
                     /locus_tag="ACHKUI_003387"
     CDS             14571..15476
                     /gene="yjhH"
                     /locus_tag="ACHKUI_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6599529.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            15487..17454
                     /gene="yjhG"
                     /locus_tag="ACHKUI_003388"
     CDS             15487..17454
                     /gene="yjhG"
                     /locus_tag="ACHKUI_003388"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HFO6599530.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDL
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTRLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            17561..18910
                     /gene="yjhF"
                     /locus_tag="ACHKUI_003389"
     CDS             17561..18910
                     /gene="yjhF"
                     /locus_tag="ACHKUI_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO6599531.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            19257..20243
                     /gene="yjhU"
                     /locus_tag="ACHKUI_003390"
     CDS             19257..20243
                     /gene="yjhU"
                     /locus_tag="ACHKUI_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6599532.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(20284..21288)
                     /locus_tag="ACHKUI_003391"
     CDS             complement(20284..21288)
                     /locus_tag="ACHKUI_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFO6599533.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(21392..22033)
                     /locus_tag="ACHKUI_003392"
     CDS             complement(21392..22033)
                     /locus_tag="ACHKUI_003392"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6599534.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(22064..23179)
                     /gene="mtnA"
                     /locus_tag="ACHKUI_003393"
     CDS             complement(22064..23179)
                     /gene="mtnA"
                     /locus_tag="ACHKUI_003393"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HFO6599535.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(23189..24448)
                     /gene="mtnK"
                     /locus_tag="ACHKUI_003394"
     CDS             complement(23189..24448)
                     /gene="mtnK"
                     /locus_tag="ACHKUI_003394"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HFO6599536.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            complement(25188..25286)
                     /locus_tag="ACHKUI_003395"
                     /pseudo
     CDS             complement(25188..25286)
                     /locus_tag="ACHKUI_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
     gene            complement(25376..25744)
                     /locus_tag="ACHKUI_003396"
     CDS             complement(25376..25744)
                     /locus_tag="ACHKUI_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6599537.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(25907..26128)
                     /locus_tag="ACHKUI_003397"
     CDS             complement(25907..26128)
                     /locus_tag="ACHKUI_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6599538.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT         6730 a         6407 c         6452 g         6615 t
ORIGIN      
        1 aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc tttctgtatc
       61 ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat tattactgtg
      121 cttttcgtcc ccattaattt ctcaggctgc gacactggac gtacgtggtg gatatcgtag
      181 tggaagccac gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa atggatggtg
      241 ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg aaaatgccgc
      301 actcaatgat gttcaggttg aagttaatta cgcgattaaa cttgatgatc aatggacggt
      361 gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa
      421 actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa
      481 agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga
      541 tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata
      601 cagcaaacag aacgattatc gctatgcaaa ccataagaaa tgggcgacgg aaaatgcatt
      661 tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg actaccttga
      721 ccgtcagggt gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt
      781 gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaacaata acggcgcttg
      841 ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat
      901 ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta ggtagcgcag
      961 gtacggcatg gtacaagctg gatacacagg ccaaagataa aaaatggaca gcgttagctg
     1021 cattccctgg cggaccaaga gatcaagcaa cctctgcatt tattgatggc aatctgtatg
     1081 tgtttggcgg cattggcaaa aacagcgagg gcttgactca ggtatttaat gacgtacaca
     1141 aatacaaccc caaaaccaat agttgggtta aattgatgtc gcacgcgccg atgggcatgg
     1201 cgggccatgt gacttttgta cacaacggca aggcttatgt tactggtggt gttaaccaga
     1261 atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca accgctatag
     1321 ataaaatcaa tgctcactat tttgacaaaa aagcagaaga ttatttcttc aataagtttc
     1381 tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg ccctggtacg
     1441 gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt aatggcgaag
     1501 ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt aataatttaa
     1561 aatggaataa gcttgctccc gtctcatcac cagatggcgt agctggcggt tttgcgggga
     1621 taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca cgagaaaatt
     1681 accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc actgatattc
     1741 atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt cgggcctacg
     1801 gagtatcatt gccctggaat aatagtctat tgattattgg cggtgaaact gcaggcggca
     1861 aagcggtgac ggattcagtt ttgatcactg tgaaggataa taaagtcaca gtacaaaact
     1921 aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttca attacgcgaa
     1981 atttatatga acgcaataat atcgcccgat tattactatg ttcttaccgt tgctggtcag
     2041 tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga tgcgcctcat
     2101 cccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc gccatgtcac
     2161 tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat
     2221 caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat
     2281 attgcacgga aattactgcc cttcattcct gataatgcag ggattcttat cgttccgtgt
     2341 tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga acggcacgga
     2401 gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga tttagtcagc
     2461 agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgc atgctggatg
     2521 caaggcgaat ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat
     2581 atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa taatattact
     2641 gacgcgccgt ggttttgcgg cgataccacc tggtactgga aagaaaattt cccgcattcg
     2701 tatgaagcta tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac
     2761 ttccagcaac aaggtgaaag aggactgacg aacgcgcctg atgaagaccc ggacgattta
     2821 agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac ggcactgcga
     2881 agcagtcatt tcagcactgc agcccgtcgg gggattattt ctgacagatt tgtagaagca
     2941 attttgcagt tttggcgcga aaggtgagcg gggagtttat tcatggggga tgtccttgtt
     3001 cgttctcaga gtagacaaca aaaaacaaat gagatcaagg taatatctct tcacttgcgg
     3061 gctcgaacag caggcaattg ccaatataat aatctggcac aagggtgaaa aacgcgcacc
     3121 gcataaaccg ttgttattgc ttacagatta gccggatgcc ttaatagaca tccgcgcctt
     3181 ttcgattatg gctcggaaat ctacgaacat ctacacagtt tactggaacg ttttgggcca
     3241 cagcgttcgc agcaccgtcc tgatatgccg ttctggcgat tataagtcga cggattctgg
     3301 caaatagacg atgatgatat gcttaatgat gttcaaagaa attaggccat tattatatta
     3361 ttgcttgcat tttctttcat tcctaaaagc acccgccatt aatcccaggc gcattatgca
     3421 aaggaaaatg attaggtaaa caataagatt tataatcagg aaaggcgact cacaataaaa
     3481 cggaaagtcg ccttaacatt ggtaaaatat gccctttact actcctgtat catgaacggc
     3541 tcatcacatt taaagctgac gttgatccag actaccatat attgccttta tttcattttt
     3601 tacgccttca ccacggtaca ccaaagacgt atcgtatcgt tgatacttat cttcccgtgt
     3661 agcactgaac cagttaaaag atccgacaca cagcagttct tcatcctcaa tcacaatttt
     3721 gctgtgaaca cgattaacca atttcgtagc gatacccatc ttattgagct tttcaacagc
     3781 atcattcagc agatgttgtt tctcctgacg cttatcatca tcgacatgcg ctatattgca
     3841 gtttttatcc gtcaccacgg taatatcaat gccccgggaa cgggccaaag ccattgatgc
     3901 cagaaatccc gtttgctcca ctttttgcca ggataaccaa ggggaaataa tcgttatttt
     3961 cttttgagct ccggcaagag tcttgttcaa aaattcatca tgctgctcca caccatgcag
     4021 cgttgaaatt tgtgtatgtg cggaaattaa atcctgtcgt ttctgaaact caaattgcaa
     4081 cgcattatta tctgaagaaa agagatattt tgccaataac cctcgcggcg aaaatgcggg
     4141 ctgcatctcg ataaggtcca tatcgccaaa taccagaaaa ctatctttag cgcgtgagac
     4201 agcaacattg aggatagtgc tgttgctatc aagaaacctg ccatcttcat gttttgagta
     4261 gacaggagaa aagagaacaa tcgccctttc cgccccctgc aaggagtgca cagtccccac
     4321 tgtcagtaaa ccctgctcgt ctttgccatt aatttccagc ttacgcaatg acattttgat
     4381 agcattaacc tgcgccgaaa atggcgtcac aacaccaaca actttgtaca gtggttcccc
     4441 gtaatggcgc tctatatcat ccttatgtgc caccagccat gctgctattg tttcagcttc
     4501 aagagggtta tgacggctcc ctccatttgg cttcatgcct ctaccatcaa tatgtaaata
     4561 tcccattgcg gggaaaatcg tcctttttcc atccctcttt taggctgcaa cttaccgtga
     4621 tagcaaagtg agttacagta gtcgatgatg ttattgaaac agcgacgatg ctcatacaga
     4681 tacattccgc gctcgaactt gggatcatac tggtagtgag agttgaactg ggctacctgc
     4741 attacgctgc cagatgctgc actttttcca gacccattta ttatcgaata cggtgaccgc
     4801 ctgagcggcc acctttaatc gtgggcagtt acacagaatt atttacaggg tcttgctggg
     4861 agttcttaca cttgctcgcg atattcaatt ggccattgtc aacaaatgac gcaggttttg
     4921 attggtgata tctatttacg tcagcaatgc gatgtgtttt ggctcggcta taccatcagt
     4981 cccgcttatg ctcgccaggg atatgcaatt gaagcaatta ccgccacgat tgactgaata
     5041 aaagagaacg gtttttcact catcaaagcc ggtgtgaatc cggaaaatac cctttcaaaa
     5101 aaattactta tcaggattgg cttcaatttt tcaagcatcg aagatgggtg agcatattta
     5161 tgttctggat ttaacaaaag gagaataatc tcccgaaggg aaaagccgat gaggccattt
     5221 caggcgtact tcaacatgca ttcgtcagtg tcatctgcct tttccagtaa atgggttggc
     5281 attcaatttc caaagtacat cacacttttt ctactgaaac atatccctcc tataactacg
     5341 tgttaatatt cgctccgtaa atacctttca actcaacccg aggctttgat gctggagaat
     5401 gtaatcatcc gataatcagt tccccgacct tttcaggccg gactgattat caatgcgccg
     5461 aaatcgaatg cggacaccgc ggtgtgtttg cacgttttgc acatgatgat taaataggtt
     5521 cttcagtatg aatttatccc gtcaggaaca acatacctta cacgttctcg ctaaaggtag
     5581 acgtattgcg cacgtccgcg attcttcagg ccgcgtcact tccgttgaat gctacagccg
     5641 cgaagggctg ttgctgaccg actgcacgct cgccgtcttc aaaaaactca aaaccaaaaa
     5701 acttatcaag tccgtcaatg gccagcccta tcgaatcaac accaccgagc tgaataaagt
     5761 tcgcgctcag ctcgataatc gctaaggaga gtttctatga cagtacacca cttcactttt
     5821 catatcacag ataaaagtga tgccagtgat atccgggaag tcgaaacccg cgcatttggc
     5881 ttcggcaaag aagccgattt agtggcctct ttacttgagg atgaaagcgc gcgccccgcg
     5941 ttatcgctgc tagcccggta tgaaggcaaa gccgtcgggc atattttatt tacccgggca
     6001 accttcaaag gagaaatgga ttcaccgttg atgcacatcc tcgctcccct ggcggtcata
     6061 ccggaatacc aagggatggg cgtgggagga cggttgatac gcactggcat cgagcacctg
     6121 aggttgatgg gctgccagac ggtttttgtt ctcggccacg ccacctatta cccccgacat
     6181 gggttcgaac cctgtgccgg agacaaaggc tatccggcac cttatcccat cccggaggag
     6241 cacaaagcct gctggatgat gcaatcactc accgctcagc ccatgaccct gaccggccac
     6301 atccggtgcg cagaccctga tgaaaccgga gcactgacgt gaataacaat cttgttgtgt
     6361 cctttttaag gcttaaccca ataacaggaa ttaaataatg gatatcccac gtatttttac
     6421 catcagcgaa agtgaacacc gcatccataa cccgttcacc gaagagaagt acgccacgct
     6481 gggtcgcgtg ctgcgcatga agccgggcac ccgcattctt gacctcggca gcggctcggg
     6541 cgagatgctc tgcacctggg ccagggatca tggcattacg gggactggca tcgacatgag
     6601 ttcgctcttc accgcgcagg caaagcgtcg cgcggaagaa ctcggcgtca gcgaacgcgt
     6661 acattttatt cataacgatg cggccggtta cgtcgcaaat gaaaaatgtg acgtggcggc
     6721 ctgcgtcggc gcgacatgga ttgccggtgg gtttgccggg gcggaggagc tgctagcgca
     6781 aagcctgaaa ccgggcggga tcatgcttat cggcgaaccc tactggcgtc agctaccagc
     6841 aacagaagaa atagcccagg cctgcggcgt cagctcgaca agcgatttcc tgacgctgcc
     6901 aggacttgtc ggagcgttcg acgatctcgg ctatgacgtg gtggaaatgg tactggcaga
     6961 ccaggaaggc tgggaccggt atgaagccgc aaaatggctg accatgcgcc gctggctgga
     7021 agcgaatcct gacgatgact tcgccgccga agtcagggcc gaactaaaca tcgcgccgaa
     7081 acgctacgtg acctacgcgc gggaatgctt tggctggggc gtgttcgcac tcatcgcccg
     7141 gtaacaacag gttcccggta tttctggctg gaaatatcga ggctttacct ttgctttgat
     7201 ctgatgtatc acctgcacaa tacatattca gtatgtttat taatcaagat gtcaccataa
     7261 ggagcatcat atgtcacaac atgaacagtt ctgtcaggct tgcggaatgc cgatgtcggc
     7321 accagatgca cacagtgcca gtgatcaata ttgtgcgtaa tgcagcgaca gcaacggcaa
     7381 tctcaaaccc tgggaagaag cggtatcagg tttagccagc ttccttgatt cctggcaaaa
     7441 agtcggcccg gaggaagcac ataagcgtgc aaaacgttat ctgacggcaa tgccagcctg
     7501 ggctcataaa gcggagagtg atttgagctg attatctata ctccctctga atctattcag
     7561 gagatccgat gttttcttac attatgctgg gaaccaacga cctgccacgc gccattacat
     7621 tctaagatcc gctgatggag atgctggggt atccacgtgc aggacgtaat gaagagggag
     7681 cgtcctgcaa caaagtggtg ctataacagt taccgcgttt tattcatcct cattatttca
     7741 accataatca ctggtctctc cagcccgtaa ctttcacaaa cgttgagcgc ttttattcca
     7801 ctcggctcac ataatttttt atttgttcag ctttgcgcgt tttcacgtga acgttctcac
     7861 aattattcga ccagtctgcg cctataaaac gctcaacgag tgattgtttt acggtctacg
     7921 gagatagcta tgtcattttc tgtgcaggaa accctgttct cgcttttgca gcacaatgcg
     7981 atttcaggac acgaaaacgc tgtcgctgac gtcatgctgt gcgaattcag gcgtcaggca
     8041 aaagaggtct ggcgagacag gcttgggaat gtcgtcgcgc gctacggtag tgataaaccc
     8101 gatgcgctgc gactgatgat ttttgcgcat atggatgaag tcggttttat ggtgcgcaaa
     8161 atagagccgt ctggattttt acgctttgaa cgcgtaggcg gtcctgcgca ggtcactatg
     8221 gctggttcca tcgtcaccct caccggggac aaagggccag tcatggggtg tatcggcatt
     8281 aagtcctacc actttgccaa aggcgacgag cgcacgcagt caccttctgt cgacaaactg
     8341 tggattgata ttggtgccaa agacaaagac gacgctatac ggatgggcat tcaggtcggt
     8401 acgcctgtca ctctgtataa cccgccgcaa ctcctggcaa acgatctggt gtgcagtaaa
     8461 gcactagacg atcgtctagg ctgtactgcc ctgctcggtg tagcggatgc tatcagtact
     8521 atggagcttg atatcgccgt ttatctggtg gcttcggtac aggaagaatt taatatccgc
     8581 ggcattgttc ccgtattacg ccgtgtaaaa cctgacctgg cgattggtat tgatatcact
     8641 ccatcgtgtg acacccctga tttacacgat tattccgagg tcaggattaa tcagggcgtt
     8701 gggatcacct gcctgaacta ccatggtcgg ggaacgctgg ccggattaat cacgcctcct
     8761 cgcctgatac ggatgttgga acagacggct cttgaacaca acattccggt gcagcgagaa
     8821 gtggctcccg gcgtgataac agaaaccggt tatatccagg ttgagcagga tggtattccc
     8881 tgcgccagtc tctctattcc ttgtcgctat acccattctc cggcggaggt tgccagcctg
     8941 cgtgatttga ctgattgcat ccgcctattg accgctctgg caggtatgtc agcagcacat
     9001 ttccccgttg agcctgattc aggcactaca caagaggcac atccattatg aaaaagatcc
     9061 ttgtggcatg cggtaccggc atgtcgactt caaccatgat tgcacacaag ctgcaagagt
     9121 tcctgactga acagggcatt tcagccacta ccgcccaatg ctgtctgaat gagatccctc
     9181 ttaactgtaa cggtatggac cttattgtaa cctccatgcg aaccaacagt gattacggca
     9241 tccctacgct taatggcgcc gccctgctca cagggatcaa cgacgacgcg ttaaaacaac
     9301 aaatcaaggc tctgttaact caataaaagg aggcattatg tttgattaca tcctgtctct
     9361 cggcggtacc gttttcgtcc ctatcatcat gatcgtgatt ggccttatct tccgcatccc
     9421 ctggctgcag gcgattaagg caggggtaac ggttgggatt ggttttgtcg gtatgggact
     9481 ggtgatcgtc atggcgatcg acagcctcag tccccctatc aaagtgatga ttgagcgttt
     9541 cggcctagcc ctgcatgtct ttgacgttgg cgcaggccct gcctccggcg ttggttatgc
     9601 gacagccatt ggcgccatga ttatcccagt tatttttctt ctcaatgtcg ccatgctggt
     9661 cacccgtctg accaaaacga tgaacgtcga tatttacaat tactggcact acgccattac
     9721 gggtacggtg gtccagctta tgaccggtag cctgatttat ggtgtgctgg gggcaatctg
     9781 ccatgcggcg ctatcgttaa aaatggccga ctggacggca aaacgggtac agaacatcgt
     9841 cgggctggaa ggtatttcga taccgcaagg ctacggttcc agttcagtac cgcttttcgt
     9901 tctacttgat gctatctatg aaaaaatccc tttcatgaag ggacgcaata tcgatgctca
     9961 ggagatccag aaacgctacg gcatggtcgg cgatccggtg attatcggcg tagtgctcgg
    10021 tctgattttt ggcctagcgg ctggtgaagg ctttaaaggt tgcgccagtc tgatgattac
    10081 cgttgcggca atcatggtgt tgttcccgcg catgatccgc ctgattgttg agggcctgct
    10141 gcccatctcc gacggtgcaa gaaagttctt ccagaaatat ttcaaaggac gtgaagtgta
    10201 tatcggtctg gatactgctg tgacgctggg gcatccaacc accattgctg tcggtctgct
    10261 actgataccc attatgttaa ttctggccag cattttaccg ggcaacaaag tgttacccct
    10321 cgcagacctg ccggtagcac cgttctttat ctgtatggct acggttatcc accgcggaga
    10381 cctggtcagg acgttgataa gcggggtcat cgtgatgatt accgtcctgc tgatcgccac
    10441 ccagttcgcg ccttacttca ctgaaatggc gctcaaaggc ggtttcagtt ttgccggtga
    10501 aagtgcgcaa atctcagcgt tgtccgtggg caatatgttc ggttggtcga tttctgaatt
    10561 gatgtcgctt gggattattg gcgttgtcgt ggcggtcggt attgtcgcca gcgtcgtgct
    10621 gttcttacgt aaacgtgaat tatcggaata aggagtactt tcaatgagtt ggctgaaaga
    10681 ggttattggg acggaaaaag cggtcatcgc catgtgccat ctacgtgcat tgcccggcga
    10741 cccgagtttt gatgcgcagt tggggatgaa ctgggttatc gacaaagcct gggacgatct
    10801 gatggcgctc caaaatggtg gggtggatgc agtcatgttc tccaacgagt ttagtcttcc
    10861 ctatctcacg aaggtgcgtc cggaaaccac tgcggcaatg gcacgaatca tcgggcaatt
    10921 aatgagcgat atccgcatcc cgtttggcgt gaatgttctg tgggatccgg tcgcctcatt
    10981 cgacctggcg atggctaccg gcgctaagtt tatccgcgag atatttaccg gtgcctatgc
    11041 cagcgacttt ggcgtctggg acactaacgt cggtgagacc atccgtcatc aacaccggat
    11101 tggtgcaggc gaagtgaaaa ccttgttcaa tatcgtccca gaggccgccg tgtatctggg
    11161 caatcgcgat atctgttcta ttgctaaatc gacagtattt aacaaccatc ctgatgcgct
    11221 ctgtgtttct ggcctgaccg ccgggacgcg taccgacagc gcgctgttaa aacgggtcaa
    11281 agaaacggtg cctgacaccg tcgtgttggc caacacgggc gtctgcctgg aaaacgtgga
    11341 ggaacaactc agtatcgccg atggctgtgt gaccgcaacc acctttaaaa aggacggcgt
    11401 ttttgccaat ttcgttgacc aggcgcgagt cagtcagttt atggagaaag tgcatcatat
    11461 acgccgataa gatggggata agcagagcgc ttatccccgg caaagagcaa taacccttct
    11521 gcgatccctg ccgccttcgt caaaagtatc gacggggatc gctaccagat taacccgaat
    11581 cggcatggag tcaacgacgg atgattaacg atattaaatg ggtacaggca caacgtaaag
    11641 ccaccgactg gcgtcaggcc gtggaaatcg ccacccgccc gctggtagcc tatggtgccg
    11701 cgcagccatg ctacgttaat gggatcatcg aaaataccct taactggggg ccttattatc
    11761 tgatagcccc aggaatcgcc ttgcctcacg cccgaccaga gcaaggcgca aactataatc
    11821 aggtcagtat caccacgcta cgcaccccgg tcgcctttgg taacgaggag tgcgaccccg
    11881 tctggctgct tctctgcgtc agcgccacgg atgccaacgc acatattttg accattcagc
    11941 gcatcagcca gtttattgat tcgccacaac gcttaacggc agtgggaaat gccagcacag
    12001 acgatgcact gtttgcgctg gtatcaggtt aacggaggcg cttatgatcc tgcacccttc
    12061 tctcgccagc gcaaatcctc tgcactatgg tcgtgagctg acggcattgg acaatctgga
    12121 ttttgggagc ctgcatctgg atattgagga cagcagcttc attaacaaca ttacgtttgg
    12181 gatgaaaacg gtgcaggccg tcgccagaca gacgcctcat cccttgtcct ttcatttcat
    12241 gcttgcccgg ccacagcgct ggtttaacgc gctggctgaa atcagacccg cgtggatctt
    12301 cgttcatgca gaaacgctgg actatccttc ggaaacgtta actgagatcc gccataccgg
    12361 cgcacgagcc ggactggtat ttaatccggc aaccccgatc gacgcctggc gatatctggc
    12421 gtctgagctg gacggagtga tggtgatgac cagtgagccc gatgggcagg gacagcggtt
    12481 tattccctcc atgtgcgaga aaatccaaaa agtacgcacg gcttttcctc agaccgagtg
    12541 ctgggccgac ggtggaatta cgctggcagc tgcacagcaa ctggcagcag ctggggcgca
    12601 acatatggtg attgggcgcg ccctgttctc ttcatctgac tatcgcgcga ccttagctca
    12661 attcgccacc ctgtaagagg agattgtttc tgatgagcca acagagacct gaccgtatca
    12721 aacagatgct gcactacctg tggcaacatc gtcacctctc cacacagcag gccatggagc
    12781 tttttggcta tgcggaagcg actgtccgtc gtgatttcca gtatattgtc aaccagtatc
    12841 caggcatgat ccgcggtcac ggttgcctgg acttcgacga cagtacagac gacaaagagt
    12901 acgtcttcga cgtgaaacgt acattgcaat ctgtggcgaa aagagaaata gctgcccttg
    12961 cccgaacaat gatcaaagat ggcgactgct tttttctcga ctccggctca acctgcctcg
    13021 aactggcgaa atgcctggcc gatgcgcggg taaaggtcat ctgcaacgac attaaaatcg
    13081 ccaatgaact gggctgtttt ccccatgtgg aaagttacat tatcggagga ctgattcgcc
    13141 caggttattt ttcggtagga gagagtctgg cgctggagat gatcaatgcc ttttccgtag
    13201 aacgtgcgtt tatctcctgc gatgcgctgt cgctggagac ggggatcacc aatgccacca
    13261 tgtttgaggt cggggtgaaa acacggatta ttcaacgctc gcgtgaagtt attctcatgg
    13321 ccgatcactc aaaatttgat gccgttgagc cgcatgctgt agcaacacta tcatgtatta
    13381 aaacgatcat cagtgacagc gggttacctg agactattgc acagcgttat cagcgagccg
    13441 gatgtcagtt gtttttacct cattcaataa agtgataagt aagttcattc gagagggatt
    13501 tcaagcaaaa ataatcaatg gcacccaata gaaaatattg gcgatgcgct cgaacgaata
    13561 aagaagctct aggcgcaatc cacacactac gatgttgcaa caacacgcca tctacttttt
    13621 attctcattc actaaatgtg gctgttctgg attcattatt caaagtgtgt acaagatcac
    13681 atttaatcac atcattacgg ttcagcatgc tgaacaaagc atattttcca ctatgtaatg
    13741 ccgataccat ttattccatg agcaaggagg agccattatg gttcgcaaag ggtgtaattc
    13801 attagtccgt gctgaaaaaa tattaaccca cattgcatgg gttggtatgg caagttatat
    13861 ggagttatta aataaattcc agtaccccaa aagcagcctg cttaatttat tgaatgtaat
    13921 ggtggattgt ggtttcttaa ttaaaaacaa aaacggctac tattcactgg gaataaaaaa
    13981 ctacgaattg ggttgtcagg cgttacaccg tcaaaatata tttgaggtga ctaagcggcc
    14041 aatgcaggag ttgtcgctga aaagtggact ggtttgtcat cttggggcga tggagagcat
    14101 ttcggctatc tatctcgaca agatcgaaag tccggactct gtaccgacga gcaaaagctg
    14161 gattggcaaa aaacttgagt tgcatattac cgcgttgggt aaggcattat tagcctggaa
    14221 gacacgagaa gaactggatt attttttaga agcactcacg ttaactccac atacgcgcaa
    14281 tacatttacc gataaaaaat tgtttctgga agaattacaa aaaacacgcc ttcggggatg
    14341 ggccatagac aacgaagaat caacctatgg ggcggtatgt ttaagtatgc cggtattcaa
    14401 tatgtataac agagttaact atgcaatctc actttctggc gatccggtag tttattcagg
    14461 aaataagata gacagctatc tggaattgct ccggaaatgc gctgagcaaa tatcatatgg
    14521 gctgggatac agaaacgaaa atgagcactt acgaaaagga aactgaggta atgaaaaaat
    14581 tcagcggcat tattccaccg gtatccagca cgtttcatcg tgacggaacc cttgataaaa
    14641 aggcaatgcg cgaagttgcc gacttcctga ttaataaagg ggtcgacggg ctgttttatc
    14701 tgggtaccgg tggtgaattt agccaaatga atacagccca gcgcatggca ctcgccgaag
    14761 aagctgtaac cattgtcgac gggcgagtgc cggtattgat tggcgtcggt tccccttcca
    14821 ctgacgaagc ggtcaaactg gcgcagcatg cgcaagccta cggcgctgat ggtatcgtcg
    14881 ccatcaaccc ctactactgg aaagtcgcac cacgaaatct tgacgactat taccagcaga
    14941 tcgcccgtag cgtcacccta ccggtgatcc tgtacaactt tccggatctg acgggtcagg
    15001 acttaacccc ggaaaccgtg acgcgtctgg ctctgcaaaa cgagaatatc gttggcatca
    15061 aagacaccat cgacagcgtt ggtcacttgc gtacgatgat caacacagtt aagtcggtac
    15121 gcccgtcgtt ttcggtattc tgcggttacg atgatcattt gctgaatacg atgctgctgg
    15181 gcggcgacgg tgcgataacc gccagcgcta actttgctcc ggaactctcc gtcggcatct
    15241 accgcgcctg gcgtgaaggc gatctggcga ccgctgcgac gctgaataaa aaactactac
    15301 aactgcccgc tatttacgcc ctcgaaacac cgtttgtctc actgatcaaa tacagcatgc
    15361 agtgtgtcgg gctgcctgta gagacatatt gcttaccacc gattcttgaa gcatctgaag
    15421 aagcaaaaga taaagtccac gtgctgctta ccgcgcaggg cattttacca gtctgaggag
    15481 aaactcatgt ctgttcgcaa tatttttgct gacgagagcc acgatattta caccgtcaga
    15541 acgcacgccg atggcccgga cggcgaactc ccattaaccg cagagatgct tatcaaccgc
    15601 ccgagcgggg atctgttcgg tatgaccatg aatgccggaa tgggttggtc tccggacgag
    15661 ctggatcggg acggtatttt actgctcagt acactcggtg gcttacgcgg cgcagacggt
    15721 aaacccgtgg cgctggcgtt gcaccagggg cattacgaac tggacatcca gatgaaagcg
    15781 gcggccgagg ttattaaagc caaccatgcc ctgccctatg ccgtgtacgt ctccgatcct
    15841 tgtgacgggc gtactcaggg tacaacgggg atgtttgatt cgctaccata ccgaaatgac
    15901 gcatcgatgg taatgcgccg ccttattcgc tctctgcccg acgcgaaagc agttattggt
    15961 gtggcgagtt gcgataaggg gcttccggcc accatgatgg cactcgccgc gcagcacaac
    16021 atcgcaaccg tgctggtccc cggcggcgcg acgctgcccg caaaggatgg agaagacaac
    16081 ggcaaggtgc aaaccattgg cgcacgcttc gccaatggcg aattatctct acaggacgca
    16141 cgccgtgcgg gctgtaaagc ctgtgcctct tccggcggcg gctgtcaatt tttgggcact
    16201 gccgggacat ctcaggtggt ggccgaagga ttgggactgg caatcccaca ttcagccctg
    16261 gccccttccg gtgagcctgt gtggcgggag atcgccagag cttccgcgcg agctgcgctg
    16321 aacctgagtc aaaaaggcat caccacccgg gaaattctca ccgataaagc gatagagaat
    16381 gcgatgacgg tccatgccgc gttcggtggt tcaacaaacc tgctgttaca catcccggca
    16441 attgctcacc aggcaggttg ccatatcccg accgttgatg actggatccg catcaacaag
    16501 cgcgtgcccc gactggtgag cgtactgcct aatggcccgg tttatcatcc aacggtcaat
    16561 gcctttatgg caggtggtgt gccggaagtc atgttgcatc tgcgcagcct cggattgttg
    16621 catgaagacg ttatgacggt taccggcagc acgctgaaag aaaacctcga ctggtgggag
    16681 cactccgaac ggcgtcagcg gttcaagcaa ctcctgctcg atcaggaaca aatcaacgct
    16741 gacgaagtga tcatgtctcc gcagcaagca aaagcgcgcg gattaacctc aactatcacc
    16801 ttcccggtgg gcaatattgc gccagaaggt tcggtgatca aatccaccgc cattgacccc
    16861 tcgatgattg atgagcaagg tatctattac cataaaggtg tggcgaaggt ttatctgtcc
    16921 gagaaaagtg cgatttacga tatcaaacat gacaagatca aggcgggcga tattctggtc
    16981 attattggcg ttggaccttc aggtacaggg atggaagaaa cctaccaggt taccagtgcc
    17041 ctgaagcatc tgtcatacgg taagcatgtt tcgttaatca ccgatgcacg tttctcgggc
    17101 gtttctactg gcgcgtgcat cggccatgtg gggccagaag cgctggccgg aggccccatc
    17161 ggtaaattac gcaccgggga tttaattgaa attaaaattg attgtcgcga gcttcacggc
    17221 gaagtcaatt tcctcggaac ccgtagcgat gaacaattac cttcacagga ggaggcaact
    17281 gcaatattaa atgccagacc cagccatcag gatttacttc ccgatcctga attgccagat
    17341 gatacccggc tatgggcaat gcttcaggcc gtgagtggtg ggacatggac cggttgtatt
    17401 tatgatgtaa acaaaattgg cgcggctttg cgcgatttta tgaataaaaa ctgaaagcat
    17461 aaataaaaac atccagacac ctgaattatc cgactctgca tcattcgtta tttgctcagt
    17521 cggataatga tgggtcgcta ccctgttatg agaggacgtt atgccactaa ttatcgttgt
    17581 ggcagggatt gctttactcc tgcttttaac cataaaaatt aagcttaata cgtttgtttc
    17641 gttaattatt gtctcgattg ctgtcgccat cgccagtggt atggatttga gtaaagtcgt
    17701 cacctcagtc gaatccggac ttggcggtac gctgggccat attggtttga tatttggctt
    17761 cggtgttatg ctcggtcgct tgctggccga tgcgggtggt gcgcagcgaa tagcgctgac
    17821 catgctgaat tatttcggga aaaataaact tgactgggca gtggtctgtt cggcgtttat
    17881 tgtcggtatc gcactttttt tcgaagtcgg tttaattctt ctggtaccta ttttattcgc
    17941 tattgcccgt gaagcaaaaa tatcaccgat gtttatgtgt gtgccgatgc tttctggttt
    18001 gctggtcgca cacggttttt tgccgccgca ccccggcccg actgttatcg ccagggaata
    18061 tggcgctgat gtaggattgg tactgatata cggtatcatt gtcggcattc caacctttat
    18121 tctttgcggc ccggtattga ataaattctg ccagcgcatt attccggacg cctttaaaaa
    18181 agaggggaat atagcctctc tcggcgcgac ccgccgattc agcgaaagcg aaatgccagg
    18241 ctttgggatc agttttctca cggcaatgct gccggtgatc ctgatggcgg ttgtcaccat
    18301 catccagatg actcatgcca aaagtgccgc ggattcaggc ttattttaca acgtgatact
    18361 gttcttaggt aattcaacga tcgccatgct gatttcactg ctattcgcga tctataccat
    18421 gggcctgggg cgcgggaaaa caatccctga cctgatggat tcttgcggaa aagctatcgc
    18481 gggtattgcc ggtctgctgc tgattatcgg tggtggtggt gcattcaagc aagttctcat
    18541 cgactctggc gttggtcagt atatttccac cctggtttcg ggtatggata ttaacccgat
    18601 cctgatggcc tggggcgtgg ccgcattcct gcgtatttgt ctgggttcag ccaccgtcgc
    18661 ggcgatctct accgccggtc tggtcatccc attgttggca gtacatccca ataccaacct
    18721 ggccttgatt acgctggcta ccggtgccgg atcctgtatt tgttctcacg ttaacgatgc
    18781 cagtttctgg atgattaaag acttttttgg cctgaccacc aaagaaacgc tgttatcctg
    18841 gacgctgatg tccacactct tatccatcag tgggctgatt tttattctgc tcgccagctt
    18901 agttttataa tcgggttctt gccagtgagc caacctgttg ttcactgtcc cccataattt
    18961 agtgagtgac ggcaaccgtc tgcacggtga cgaatcgctg gcgatcaaaa aggggcgata
    19021 ttgaaccttc gattatgctt gatgggtgta ttctcagtaa tactgtaaag ccggagcatg
    19081 tctccggctt tacagtatgg gggaacagcg ggcgatcgct caaatggtat gctggtttat
    19141 ttcatgtaaa agaataaaat atctctataa aatcatgcca ctggcaatct gcatcaactc
    19201 tccgggtaaa cagggttaag atgagcattt ccccggttta atacaggtat acaaaagtgg
    19261 atagagatca aacaaacagc agcctgttca atgatgatcc cgtacttcat gcaacatggc
    19321 tgtactatca ggaaggtaaa agccagactg aagttgcagc tatcatgggg gtgtcccgcg
    19381 tcacggttgt caaatacctg caaacagccc gggaaaatgg actggttcat atcaacctgg
    19441 atgtgaatgt ctttggctct atcgacgctg ctttgcaaat tcgcgataaa ttcaatctcc
    19501 agagagtgat catcgtgccg gatggcgaac atgccggaaa acgtgatgat acaaagctga
    19561 tgcggactcg cctttcacgt gccggtggca tgtatctgaa tcaggtgatt gagaatggtg
    19621 atgtgctggg ggttgcctgg ggaagaacaa tccatcagat gagcaaaacc atgacgccga
    19681 agtcatgtaa aaatgtcacc gttatacaga tgctcggttc catgccatct cagccagacc
    19741 tgacgatcat tgaatcctca tcgcaaatcg cctacaagct ttcaggccgt gtcgcctcgc
    19801 tgcacgtgcc cgccgtggta tcaagcgcca gactggccat ggagttacag gcagagccta
    19861 ttattcgatc gaattttgac gttctgactc gctgtacgaa agcctttttt gtcgtgggta
    19921 acgcgcttga tgaaaatccc ctgattcggg tgggcgtgct gaataagaag gaaatgcaga
    19981 cgtatcggga tttaggcgca gtaggtgtca tttgtggtcg cttctatgac aaggaaggga
    20041 tgcccgtcgt ggctgatgtt gaccagcgta ttctgggaat cagtctggct cagctaaggc
    20101 agattgaacg gaagatcttc cttgctggtg gggaaagagg ctatgacgca acgttgggtg
    20161 ccctgctggg aggatatgtc acggacctga ttgttgatga aggaacggca gagtttttgc
    20221 tggcgtgtga attaccacac taaaagaatg aacaactgcc ccatgtagat atggggcagg
    20281 attttattga ttagcggcga taataagaac aatacccaca atgtatgaaa taaacatggt
    20341 cagcagcagt ttattggttc cggctggcgc actggcaaat tctctccaga tgaagactcc
    20401 ccagatcacg gcaatcattg tggccccctg tcccagaccg taggaaatgg cataccccgc
    20461 ctgaccagac gcaatcagac tgaatgccag accgacacac caaatcatac cgcccagcca
    20521 gccgcaaaca tgatcccgta aactcccgga aaaatacatt ttcccgttga ccggttctcc
    20581 tgaaataggt tttttcatga ccagcctgtt aaggacaaag ttcgacaaaa acagccccac
    20641 ggcaaacaga acaagtgctg agtaaggcgt catcagaccg ctggcaggct ggctgaaatt
    20701 gtcagacatg gagtccgcca ggaagcggaa aaaccatccc atgatgatac ccgccagaat
    20761 ggcagtaatg agacctttat tacggcgaga tttatccgcc tcctgcgtga cacgaccata
    20821 ggctatcgcg gtaaaaataa tggcgatcac aacgcagacg acaccgagga acagaatgag
    20881 cggatcgccc tgcggattgc cgatataggt ggtgatcaca cccagagcca gtgccagccc
    20941 cacacccaca ggaaaagcta ccgccatacc ggcaagatta atagaagcga ccagaagaat
    21001 attggaaata ttgaagataa tccctgccag tattgccgac atcagactac tgctgctcgc
    21061 ctgctgaata tcaggtataa aactgcgacc ggcttcgccc atagaaccca gcgtgaaggc
    21121 aaataacaaa gaacacagca ataatccaat tgaataatcc caatagaata gagggaactc
    21181 ccatttttta ttgctgacaa gtttagttgt attcgcccat gaaccccagc aaatcatggt
    21241 aataaaacac attattatgg cgacagcata cgattccacg ataaacataa ataactcctt
    21301 gtggtagtta taatcaatcg ttgtggacag agggaatagc agccattctg cgaatggctg
    21361 tcatatgtta ttaattaaaa taaagctgta attacgcttt caaaccatac gttttgaatt
    21421 tttcaaggac gatggtcatt tcggcttcag acagcacggg aacatcgtga tgtaatccgc
    21481 cacatttgat gtataaatca gccagcgtct cggtttcacc cgccagccac aacgtctttt
    21541 ccagcgtgac gtccatggcc agcataccgt ggtgctgcat cagcaaggac ttactttctc
    21601 tgattccttt agatacaccg tcggccagtt caggactgcc aaaggtataa tagggaatac
    21661 agggaatatg atccgtcccc gataccgcca ccatataatg aatggccgga attgaatgat
    21721 tatgtattgc caccgccgtg gaattcaccg catgcgtatg tatgacagca ttcaattctg
    21781 gtctggcttt ataacacgcc agatgaatta accattcact tgatggtatt ttacccgctt
    21841 caggttttcc cttatcgtct acaaagacaa tcatgtccgg cgtcatcttt gaataagcga
    21901 tcccactcgg cgtaatcaac atgccaccgg tatacctggc actcacatta cctgatgttc
    21961 cttgattaag acctgacgca ttcatttccc gacaagtgga aatgattttt tctgctaact
    22021 taatccgttc cattcctgat ccttaatagc aatggccgct gttttaatgc tgttccccct
    22081 ggagtgcttt actcttcagg tcagcaaaca tatccgccag cgctgactta ctggcagcac
    22141 agactccccg ctcagtaatg aagccggtaa tgtatcgggc aggcgtgacg tcgaaagcgt
    22201 agttcccaca acgggttcct tcaggggcgg tattgaccca gctcaattct ccctgagggt
    22261 ttatcccata gacatgggac tgctcttttc cgtcacgttg ctcaatcgga atgctttttc
    22321 cgtcctcgat agtccagtca atagtgggag aaggtaacgc gacgtagaag ggaacatggt
    22381 tatcatgggc cgccagcgcc ttaagatagg taccaatttt attacagaca tcccctctgg
    22441 ccgtggttcg atcggtccca acaatgcaga gatcaacgtc accgtgttgc atcaggtggc
    22501 cacccgcgtt gtcggcgatc agggtgtgag gaataccgtg tgagcccagt tcaaatgccg
    22561 tgagtccgcc ctgattacgt ggccgcgttt catccaccca gacgtgaaca gggattccgt
    22621 tttcatgcgc tttgtagatg ggagaaagcg cggttcccca gtctacagtc gccagccagc
    22681 ctgcgttaca atgtgtcagg atattgacca cactcccggc cggttttttc tgggctattt
    22741 cgcggatgac ttctgcacca tgtaatccaa tttgttcaca caacgcgaca tcttcctcgg
    22801 caatttctgc cgccagcgcc caggccacat ccttgcgctc tgatacacag agatccttca
    22861 gtgtgtctat catcctgtcg agggcccatt tgagattaat tgccgttggt cgggttttga
    22921 taagaaggtc atagtaacgc tgcagaccca tgtcactggc atcgtgattc attcccagcg
    22981 cgatgccata ggctgcaacg acaccaataa gcggagcgcc acgaacccac atatcctgaa
    23041 tagccgtagc ggccatttcc gcagaggtaa gcgccaccac ctcgaactta aaaggcaact
    23101 tagtctgatc gatgatttct accgcttttc catccccgga aacccagacc gtacgatagt
    23161 gtttaccttt aatattcata atgacctctt acttaacctt cgcagactgg atgacggaat
    23221 ccatattttt aaactggcgg gcattgacga taagatcgcg cgccatggtc aatgcacgtc
    23281 gttcacatcc tgcgcgaagc tctgtatttt caatctgttt cagctcggca acgccagcaa
    23341 aaccaatgat ccggcgattc atttccatgc cggcattcac cagggaatct tcaagcagcg
    23401 tggcaaaaaa ctgatcctgt gcgtcctcaa gagcggccct gttctgttga tacatttctg
    23461 tcgaccacgc gtcgccctgt gttttgttgt cccagagctg gcggaactcc cgggtgaaaa
    23521 cggaccaggt ttggacaatc tgctgaagca gccattcctg atagtcagcg cgacgttgct
    23581 cattcgcatc ccacccaggg cgtgagaagt acgccagcag gaggttgcca atatagttgc
    23641 cgatatcaaa cgccattggc cccatgaaac cgaactccgg atcaatcact ttggtatccg
    23701 tgtcggtcac catgattgag cctgaatgaa ggtcgccatg taacaatgcc tgcgcttctg
    23761 tcataaattt gtacttataa cgcatggcaa cctggatcat ctctacatca gcccaggcct
    23821 tatggacggc atcgtccagc tcaggagaag tccagttatt ccgttcagcg ttgtaatagg
    23881 gctctgtgaa gatcagatct tccgtaattt tgcacagctc atgattcaac gcgaactcgg
    23941 cagtaagcgc ttttttctgt tctgctgaca tgccaatgtc agacgtattg aagagagtct
    24001 gtgctaaaaa tctgccgata tcttcagcta gtttagggaa ttttttaccg ttaatcagtt
    24061 ccttacgcag aatcacgtga ggagtcagat actccatggc aaacagcgcc atttccgggt
    24121 cgtaaaaata gacctgcgga atcagtgtgt gacccgcgta cttggcttcc tgacataaga
    24181 cgttgtactc aaagcccgca cggctcagag ataaaggcca tccttcgccc ccggcacgaa
    24241 gccagggcag agcttgtttt acaatgatgg ttttatgtgt cccctcaacg ataaacacca
    24301 tgttcaggtt tccgtccccc acttctgaaa ctttccactc ttcaggtaac cctcccggtt
    24361 cgcatgaagg ggtcagtctg gacgagagat aacccggcag cgtgtcacat gtcagcggct
    24421 tgtaacctga tggaattgaa tccgtcataa cagttctcct tgtctacaca gaatgataat
    24481 caacttgtca tgtgacaact atacattatc aaatgtaaaa ttatttgtga cacatgtaga
    24541 ttttttgaac tgtataaaat taaaaatgat gtcgtatgga gtcatgatcc ttacaggaat
    24601 cagcagagga aaacatctat tattaccatg taattcatgt tgttatgtcg caacaatagt
    24661 actgcacggg gaattattac gggcaaactt aggcgaaagg aagtactgaa gtgacagaat
    24721 aaatcacact ttttgtacat atgtactttt ctattgtctt ttgttgtgga aacaaatggt
    24781 gtttgtcatg ccgatgctaa tagacagata gattcgaggg ccaacagccc catcatcatg
    24841 atggagcaac agaggtgagt ctaacgtttc cgcacttctt atcgatcggc actaacgatc
    24901 aatatcgata aactgatcta tcaaaacgat tatctttctt gcaacctgcc tagttatcct
    24961 tctcttacat tgctcgcaga aaagcccttt tattacggtt agttatctag ctgaggcttg
    25021 ctacgcccat tttccatgta ccccaaatca cgaggtcaca aaggcgcgga aagatgaagg
    25081 gatgtgcacg ttgcaacaaa aaaaagaagc gtccttgaac agaggctctc actcgataaa
    25141 gaattctgcg acgagtgctt attcgatatc gatgaggaaa tcagggaatg ctggtccagt
    25201 aaccgggtga gcagtgtctg ccagatggtg actggagacg ggcagcacga tacctcccgt
    25261 acatgcgtgt cgggtaatgt tttcatactg aagattttcc tgatatgcag agataaaaaa
    25321 tgggacagtg gcgtggtgaa aacaccaggc cggagcagaa ggttattctg gtggtttact
    25381 ttttcgtttc aggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt
    25441 cggcttcaca ggtcagcccc ttcgcataca gtgtcacggt atgctgatgg cggggattca
    25501 gttcaccact ggtgagcatt aatttcagtt gtttcatcag cagcggaaag gcctggtcca
    25561 gagggtacgc atccgcatct ctgaaccggc ctctgatacc ggcacggtcg gcaaggtaat
    25621 gcaaccggtt accctcctgc accagacggg cgccgaaaca gggcgtcact gtgcagggta
    25681 gtccccacca gggatggtcg tcattgtcat cgggatgcgt tgtcccggaa agtgtgtctg
    25741 acacgataaa atcctcacag aaaatcggtg aatgatagtt taacgatgac gggtcagccg
    25801 gcgcgcagac cggcttcggt aaaggtgtat tcgttgatga gcaggacctc atccacagcg
    25861 gcgtctgagc tctgccagtc gtactcgttt tccgggtgac ggaacatcag ttcagggtga
    25921 cgctcatcag ccggacatac ggaccaaaac tgtccttacg gcgttctgca aacacggcca
    25981 gcacaccggg aatatcctgc acttcacgac cggtatacgc ttcagcactg ccgtgccagc
    26041 ggtatttgcc ggtacagaac ggaaaaagac gggatgccgg atgctgacgg tgaatacgca
    26101 tggcttcgcc acgggtgatg attttcatag tgggatacct ctgaagacag aagataaaag
    26161 tgaaaacagg tgtgatgtgg ttgtgacggg ttaaagcaga ccat
//
LOCUS       DAZFRE010000075        25478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFRE010000075 DAZFRE010000000
VERSION     DAZFRE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.075"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKUI_003398"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKUI_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6599539.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKUI_003399"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKUI_003399"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6599540.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKUI_003400"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKUI_003400"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6599541.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLTRQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKUI_003401"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKUI_003401"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6599542.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAIYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGIDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKUI_003402"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKUI_003402"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6599543.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="ACHKUI_003403"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="ACHKUI_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6599544.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11233
                     /gene="ftsK"
                     /locus_tag="ACHKUI_003404"
     CDS             7244..11233
                     /gene="ftsK"
                     /locus_tag="ACHKUI_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6599545.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPHQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQQQYQQPQQPV
                     AQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGVGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            11392..12003
                     /gene="lolA"
                     /locus_tag="ACHKUI_003405"
     CDS             11392..12003
                     /gene="lolA"
                     /locus_tag="ACHKUI_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6599546.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12014..13357
                     /gene="rarA"
                     /locus_tag="ACHKUI_003406"
                     /gene_synonym="mgsA"
     CDS             12014..13357
                     /gene="rarA"
                     /locus_tag="ACHKUI_003406"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6599547.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13448..14740
                     /gene="serS"
                     /locus_tag="ACHKUI_003407"
     CDS             13448..14740
                     /gene="serS"
                     /locus_tag="ACHKUI_003407"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6599548.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKFIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            14979..17423
                     /gene="dmsA"
                     /locus_tag="ACHKUI_003408"
     CDS             14979..17423
                     /gene="dmsA"
                     /locus_tag="ACHKUI_003408"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6599549.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYGIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGIPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILLDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKLRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17434..18051
                     /gene="dmsB"
                     /locus_tag="ACHKUI_003409"
     CDS             17434..18051
                     /gene="dmsB"
                     /locus_tag="ACHKUI_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6599550.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18053..18916
                     /gene="dmsC"
                     /locus_tag="ACHKUI_003410"
     CDS             18053..18916
                     /gene="dmsC"
                     /locus_tag="ACHKUI_003410"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6599551.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAESQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(18952..19578)
                     /gene="ycaC"
                     /locus_tag="ACHKUI_003411"
     CDS             complement(18952..19578)
                     /gene="ycaC"
                     /locus_tag="ACHKUI_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO6599552.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19892..21040
                     /gene="ycaD"
                     /locus_tag="ACHKUI_003412"
     CDS             19892..21040
                     /gene="ycaD"
                     /locus_tag="ACHKUI_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599553.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLAPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(21137..21877)
                     /gene="pflA"
                     /locus_tag="ACHKUI_003413"
     CDS             complement(21137..21877)
                     /gene="pflA"
                     /locus_tag="ACHKUI_003413"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6599554.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(22069..24351)
                     /gene="pflB"
                     /locus_tag="ACHKUI_003414"
     CDS             complement(22069..24351)
                     /gene="pflB"
                     /locus_tag="ACHKUI_003414"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6599555.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(24406..25263)
                     /gene="focA"
                     /locus_tag="ACHKUI_003415"
     CDS             complement(24406..25263)
                     /gene="focA"
                     /locus_tag="ACHKUI_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6599556.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6329 a         6328 c         6686 g         6135 t
ORIGIN      
        1 ttgttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg aataaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttctaaagt actccataca tatcacaaaa
      181 taaaaaggcc ggctaaaccg accttttact cattctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgaggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacgg ataaattcat cacagaagac cagaagcgcc gttttggacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag tgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcgatg acctgaccaa aagcgtaatt catcgtgaca cgatagggtg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc cataacaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgccagg agaaaaccac gggaaaatac
     1321 cacgctgata agccattaac aggcgcgcag ggctaagatc gccccccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggtaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc gcacatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgcca gagcctcatc actactacca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgggga acaacgctaa tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg actatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagataa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagcgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccggacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtgag ttcagattga cggcgttgcg cttccagcca
     2521 ctgaatttct gtattctcta attgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcacc taccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagt aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtatatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gctaccgctg aggcggagag gaaccagccg gaaagggtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc cgccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactgatg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcaccaaca ggcgtatcaa tgccttgcgg caggagcggt agaaactcac tgacccaggc
     3601 gttatccagc gctgtttgta actcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacactgct cgttggcctg ctggcaaagt aaaattcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ttcaatggtc accggatcgg tcgatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcaaactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggctag aaaacccgca gccagcgtca caccagtatc
     4141 gtagtgacca aaatccagct cgccgaggta ggaaaaacca aagtagatcg ccaccagagc
     4201 aatcgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaat aacggaatta gcggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atagccacca caatcagcag cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagcacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaacagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaat tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgacttg
     5101 taaaattatt ttgcgtcagc taaaccgtcg aggtagcgtt ccgcatcaag agctgccatg
     5161 cagcctgtac cagcagatgt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatctga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa taatgttgcc gttctccact ttatccatca gacgcttaat gaggattttt
     5521 tccgcacgga aaccatcacg gcggtgaatc agatgaactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgc tatcgccagt cagacggaac ggacgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg gcaggattgt agggaattta
     6121 cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg cagcatcgat
     6181 aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc gctttataag
     6241 ccgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg tactaaaaat
     6301 cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccatgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atccggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtctg cttaccgtaa gttactgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgtcttc tggaggcgtt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggcag cgggcctgac gctgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa tattcttacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga gtatgaagac gacgaagagt atgaagatga
     7921 aaatcacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag atgctgcgct
     8041 gttctccggt aagcggatgg atgatgaaga agagattacc tacactgcac gcggtgtggc
     8101 tgctgaccct gacgacgtcc tgttttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gttgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcacc ggttgaacct gtgactcaga cgccgccagt
     8281 tgcctctgtt gatgttccac ctacgcaacc tacagtagcc tggcagcctg taccggggcc
     8341 gcaaacggga gaaccggtta ttgcacctgc accggaaggt tacccacacc agtcacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa cctgtacagc cacagcagcc
     8461 gtattatgcg cctgcggctg aacaacctgt gcaacaacct tactacgctc ctgcggcgga
     8521 acaacctgtt cagcaaccgt attacgcccc tgcgccagaa caaccggtgg caggtaacgc
     8581 ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc agacggagca
     8641 aacttatcag cagccagctg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca
     8701 gcagcctgtt gtggagcctg aacccgttgt agaagagaca aaacccacgc gtccgccgct
     8761 ttactatttt gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg
     8821 gtatcaaccg attccagaac cggttaaaga accggaaccg atcaaatctt cgctgaaagc
     8881 accttctgtt gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc
     8941 tggtgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt
     9001 cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt
     9061 gccacgaccg aaacgcatcc gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa
     9121 gctgccctca cagcgtgcgg cagaagaaaa agcccgcgaa gcccagcgta atcagtacga
     9181 ttctggcgat cagtacaacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg
     9241 tcagttcgcc cagacgcagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt
     9301 gaacacagaa gatgcagatg ctgcggcaga ggcagaactg gctcgtcagt ttgctcaaac
     9361 tcaacaacaa cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga
     9421 ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac
     9481 gccaattgtt gaacctgtac agcagccgca acaaccggta gcaccgcagc agcaatatca
     9541 gcagccgcaa caaccggtag cacagcagcc gcaatatcag cagccgcaac aaccggttgc
     9601 accgcagcag caatatcagc agccgcaaca gccggttgca cagcagccgc aatatcagca
     9661 gccgcaacag ccagttgcgc cgcagccgca tgataccctg cttcatccgc tgttgatgcg
     9721 caatggtgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
     9781 gacacctccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg
     9841 cctggtagaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
     9901 ggggccagtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cagcgcgcat
     9961 ttctaacttg tcacgtgacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    10021 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    10081 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    10141 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    10201 cttgttggtt gcggggacta ccggttccgg taagtctgtc ggggtgaacg cgatgatcct
    10261 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    10321 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttaccgatat
    10381 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    10441 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    10501 taggatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    10561 gcatccggtg ctgaaaaaag aaccgtacat tgtggtgttg gtggacgaat ttgccgacct
    10621 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    10681 tgccgcgggt atccacctcg tactggcaac gcagcgtcca tcggttgatg ttattactgg
    10741 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    10801 acgtaccatt cttgatcagg ctggtgcgga atcactgctg ggtatggggg atatgctcta
    10861 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    10921 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    10981 cacctccgac agtgaaagcg aaggtggcgt gggcggtttc gatggcgctg aagaactgga
    11041 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    11101 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    11161 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc
    11221 gccatttgac taattaatgc aacgtagacc ggataagacg cgatagcgtc gcatccggta
    11281 ttctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat
    11341 agagagtaga gggaactccc gattgggagt gacgtaattt gaggaataat gatgaaaaaa
    11401 attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc
    11461 gcaagcgatc ttaaaagccg cctggataaa gtcagcagct tccacgccag cttcacacag
    11521 aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa
    11581 cgtccaaact tattcaactg gcatatgact caacctgatg aaagcattct ggtttctgac
    11641 ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac ctggctgaaa
    11701 gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag cgactggcag
    11761 cagtacaata tcaaacagaa tggcgatgac tttgttctga cgccgaaagc cagcaatggc
    11821 aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg
    11881 gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa tggggctgtg
    11941 gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag
    12001 tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct
    12061 ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac atttgctggc
    12121 tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta tgatcctctg
    12181 ggggccgccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc
    12241 tgatgtggaa cgtatttctg ccgtcacctc tggtgtgaaa gagattcgcg aggcgatcga
    12301 gcgcgctcgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca
    12361 ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac
    12421 ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg
    12481 tgcccgtgtc tatctgttga aatccctgag tacagaggat attgagcaag tactaactca
    12541 ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc cagatgaaac
    12601 acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga
    12661 aatgatggcg gatatggccg aagtcgatga tagcggtaag cgggtcctga agcctgaatt
    12721 actgaccgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg atcgctttta
    12781 cgatctgatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctcta
    12841 ctggtatgcg cgaattatta ccgccggtgg cgatccgtta tatgtcgcgc gtcgttgcct
    12901 ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg tggcaattgc
    12961 ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc
    13021 gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc
    13081 gctggccgat gctcgcgaac gtccggatta tgacgtgccg gttcatttgc gtaatgcgcc
    13141 gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc atgatgaagc
    13201 aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta
    13261 tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct ggctggctga
    13321 acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt
    13381 taatgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag
    13441 gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact
    13501 ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgagg agcgtcgtaa
    13561 agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat cgaaattcat
    13621 tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact
    13681 gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga
    13741 tatcgcgcta accatcccta acctgcctgc agatgaagtg ccggtaggta aagacgaaaa
    13801 tgacaacgtt gaagtcagtc gctggggcac cccgcgtgag tttgactttg aagttcgtga
    13861 ccatgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagccgcag ttaagttgac
    13921 tggttcccgc tttgtcgtca tgaaagggca gattgctcgc atgcaccgcg cactgtcgca
    13981 gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg ttccgtacct
    14041 ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt
    14101 ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc
    14161 agaagttccg ctgaccaacc tggtacgcgg tgaaatcatc gatgaagatg atctgccaat
    14221 taagatgacc gcccacaccc catgtttccg ttctgaagct ggttcatatg gtcgtgacac
    14281 tcgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc
    14341 ggaagactca atggcggcgc tggaagagat gaccggtcat gcggaaaaag tcctgcagct
    14401 gctgggcctg ccgtaccgta aaatcatcct ttgtaccggc gacatgggct ttggcgcttg
    14461 caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgcg agatctcttc
    14521 ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc gtagcaagtc
    14581 ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtcttgctg ttggtcgtac
    14641 gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt
    14701 tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct
    14761 aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata attactcctc
    14821 acttacaagt aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaattat
    14881 tcgatgtata caagcctata tagcgatctg ctatagaaat aattacacaa tacggtttgt
    14941 tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa atccctgatg
    15001 cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc
    15061 tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata
    15121 gcgctattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg
    15181 gtagtcgctg cccgctacgt atgcacgtag tggacggtga aatcaaatat gtcgaaacgg
    15241 acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgt ctgcgtgggc
    15301 gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg
    15361 gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcttac ggcatcatcg
    15421 cgaccaatat gcagcgcttg atcaaagagt acggcaacga atccatctat ctgaactatg
    15481 gcaccggtac gctgggtggc accatgaccc gctcgtggcc gccgggaaat accctggtcg
    15541 cgcgcctgat gaactgctgc ggcggttatc tgaaccatta tggcgactac tcctccgcgc
    15601 aaattgcgga aggtctgaac tatacctacg gcggctgggc agatggtaac agcccgtcgg
    15661 atatcgaaaa cagtaaactg gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga
    15721 gtggcggtgg ggtgacttac tatcttgaac aggcgcgcca gaaatctaat gctcgcatga
    15781 tcatcatcga tccgcgctat accgacaccg gtgccgggcg tgaagatgag tggatcccta
    15841 ttcgtccggg aacagatgcc gcactggtta acggtctggc gtatgtgatg atcaccgaaa
    15901 acctggtaga tcaggaattc ctcgataaat attgcgttgg ctacgacgag aaaaccctgc
    15961 cagccagtgc gccgaaaaat ggtcactata aagcttatat tctgggtgaa ggacccgatg
    16021 gcgtggctaa aacaccggaa tgggcttcgc aaattaccgg tatcccggca gataaaatca
    16081 tcaaacttgc tcgtgaaatt ggtagcacca aaccggcatt tatcagccag gggtgggggc
    16141 cgcagcgcca tgccaacggc gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga
    16201 ccggtaacgt tggtattaac ggggggaaca gcggcgcgcg cgaaggttca tacagcttac
    16261 cgtttgtccg tatgccgact ctcgaaaacc cgattcagac cagcatttcc atgtttatgt
    16321 ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga
    16381 aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta
    16441 atcagcattc tgaaatcaac cgtacccatg aaatcctcct cgatgataag aagtgcgagc
    16501 tgattgtggt tatcgactgt cacatgacct catcggcgaa atatgctgac atcttgctgc
    16561 ctgactgcac tgcttccgaa cagatggact ttgcactgga tgcatcctgc gggaatatgt
    16621 cttacgttat tttcaatgat caggtcatta aactgcgctt tgaatgtaag actatctatg
    16681 agatgaccag cgaactggca aaacgtcttg gcgtcgaaca acagtttact gaaggtcgta
    16741 ctcaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc
    16801 caacgtttga agagttccgt aagcagggga tctttaaaaa gcgcgatccg caagggcatc
    16861 acgttgctta caaagccttc cgtgaagatc cgcaggctaa tccattgacc acgccatcgg
    16921 gcaaaattga gatctattca caggcgctgg ctgacattgc cgctacctgg gaattgccag
    16981 aaggtgatgt gatcgatcca ctgccgatct atacgccggg ctttgaaagt tatcaggatc
    17041 cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct cgcgttcact
    17101 caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg tggatcaacc
    17161 cgcttgatgc gcaaaaacgc ggtatccaca acggcgataa agtcaggatc tttaacgatc
    17221 gtggtgaggt ccatattgag gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac
    17281 tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggc ggttgtatta
    17341 acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc
    17401 ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt
    17461 gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat
    17521 ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggtggcga ctggcaggaa
    17581 gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc atgtaaccac
    17641 tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgtgaagat
    17701 ggttttgttg tggtcgatga agatgtatgc attggctgcc gttactgtca tatggcttgc
    17761 ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt
    17821 tatgaccgtg ttgcggaggg aaaaaaaccg atctgcgtag agtcctgccc gctgcgggcg
    17881 ctggatttcg gccctatcga cgagctgcgt aaaaaacacg gcgatctggc ggctgttgcg
    17941 ccgttgccgc gtgcgcactt caccaaaccg aatattgtga tcaaacctaa cgccaatagc
    18001 cgcccaaccg gggataccac tggctatctg gcaaacccga aggaggtgtg agatgggaag
    18061 tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tagcgggtgg
    18121 ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaat cccagcagcg
    18181 tgttatcgcc tgcatgtttg gtttatgggt gctgatgggc attggcttta tcgcctctat
    18241 gcttcatctt ggttcaccga tgcgcgcttt taactcgctc aatcgggtag gggcttcagc
    18301 actcagtaac gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt
    18361 gctggcaatg ctgaaaaagc tttcaccggc attgcgtacg ctttggctgg tggtgacgat
    18421 ggttcttggc gtagtctttg tctggatgat ggtgcgtgtg tataacagca tcgatacagt
    18481 acccacctgg tacagcatct ggacaccgat gggcttcttc ctgacgatgt ttatgggcgg
    18541 cccgttactg ggttacctgt tgctgagtct ggctggtgtc gatggctggg cgatgcgtct
    18601 gctgccagcc atttctgttc tggcactggt agtgagtggc gtgatgtcag tgatgcaggg
    18661 cgcagagctg gcgactattc atagctctgt gcagcaggcc gcagcgctgg tgccggacta
    18721 tggtgcgctg atgtcctggc ggattgtgct tttggccgtt gccctgtgct tgtggattgc
    18781 accacagcta aaaggttatc agcctgcggt tccgctactt tctgtatcat tcattctgct
    18841 tctggcaggg gaattaatcg gtcgtggcgt attctatggt ttgcatatga ccgtggggat
    18901 ggctgtcgca agttaatcag taacaaccgg ggtttcggcc ccggttcgct tttatttctg
    18961 cttcgttaac gtgtcataac tggtcatcaa gttacgataa tcaggaatgt ggttagagaa
    19021 caacgtcgcc agtccttcaa tatcattacg ccagtcgcga tgcagctcgc atgccacgcc
    19081 gaaccatgtc atcaattgcg caccagcttg cgacatgcga tcccatgccg aatgccgggt
    19141 aatttcatta aaggtccctg acgcgtcagt cacgacaaag acgtcaaacc cttcttcaat
    19201 ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa taattaactg
    19261 ttttttacct gtcgctttga cagcttttac aaaatcttcg ttatcccagg cgttaatatt
    19321 tcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg gaactaatgg
    19381 gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga aatacttagc
    19441 caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat cccgtacaag
    19501 ggaaagtaaa ccagcctggt gatcaacaag caaaacggca gcatcatttt tatcaagacg
    19561 aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc taaatcggtc
    19621 agacgtgatg cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa
    19681 atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga
    19741 gattaatcgt ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc
    19801 gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca
    19861 tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct
    19921 tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgcgcc
    19981 gctttggctc gcccaggaac acatgtctac atggcaggta ggcgttgtca gctcatccta
    20041 ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt
    20101 taaccgtagc tattatctgg cctccttcat ttttgccgct ggctgtgccg gccttggcct
    20161 gatgattgga ttctggagtt ggttggcgtg gcgttttgtc gctggcatcg gctgtgccat
    20221 gatttgggta gttgttgaga gtgcgctgat gtgcagtggg acgtcgcgta accgcgggcg
    20281 gttgcttgct gcgtatatga tggtttatta cgtcggaacg tttttaggcc agttactggt
    20341 cagcaaagtc tcaaccgagt tgatgagtgt attgccgtgg gttacaggtt tgacgttggc
    20401 aggaatctta ccgctgttgt ttacgcgtgt cctgaatcag cagactgaaa accatgattc
    20461 gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat
    20521 tatctcagga atcgtgctgg gatctctata tggcctgatg ccgctgtacc tcaatcacaa
    20581 aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg cgggtatcct
    20641 cgggcaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg tgttgcgtgt
    20701 tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc
    20761 agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc
    20821 ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac tgttgagcta
    20881 taccgtggga agcctgcttg gcccgtcatt taccgctatg ctaatgcaga atttctccga
    20941 taatttatta tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg
    21001 taacgccggt catacgccga aacccgtggc tcacgtgtaa atgaattcaa gcagagtgtg
    21061 aacttactgt ttcacactct gcttttctgt gcacaaaaaa gcggtaactc aataaagttg
    21121 ccgctttacg gggaaattag aacatgacct tatgaccgta ctgctcaaga atgcctttca
    21181 cgcgttccat ggtctctttc ttcggtggtt taacaccatc gagtttgtat tcttcaccca
    21241 tcgccaccca tttgtgtttg cccagttcgt ggtaggggag aagttcgatt ttctcaacgt
    21301 tacccatatc acgggtaaat tcaccgaggc gatgcgctga atcgtcatcg tcagaccagc
    21361 cagggacaac aacatagcgg atccacacct tcacattttt gttcgccaga tatttagcga
    21421 actccagcgt gcggtggttg gaaactccaa ccagattttg gtggatctcg tcgttcatct
    21481 gtttgagatc gagcattacc aggtcggtta cttccagcag ttcatcaatc accggatcgt
    21541 aacgacgaac aaaaccgttg gtgtccagac aggtatgaat gccttctttt ttgcaggcgc
    21601 ggaaccagtc acgaacaaac tcagcttgta ggattgcctc accgccggat gcggtaacgc
    21661 cgccgccgga agcgttcata aagtggcgat aggtcaccac ctccttcatt aaatcttcaa
    21721 cggtaacttc tttaccgcca tgcgtgtccc aggtgtcgcg gttatgacaa tacaggcagc
    21781 gcatcaggca gccctggaaa aaggtgataa agcgaatacc cgggccgtct acggttccac
    21841 aggattcaaa ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc cagatgtggc
    21901 ccatctgagg ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca
    21961 ccttaaagta tagatagctg acaaaaaagg ctctcgtgct aaaaaaggcc ccactttcgt
    22021 ggagccttta ttgtacgctt tttactgtac gatttcagtc aaatctaatt acatagattg
    22081 agtgaaggta cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac
    22141 tgcgtagcca gatacacgga tggtcagctg cggatatttt tccgggtttt ccatcgcgtc
    22201 gagcagcatt tcacggttca tcacgttaac gttcaggtgc tgaccacctt cgatggatgc
    22261 ttcgtggtgg aagtaaccat ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc
    22321 tttacccagt gcgttcggaa cgatagagaa ggtgtaggag ataccatctt tagcgtaagc
    22381 aaacggcagt ttagcaacgg aagtcagaga cgctacagca cctttctggt cacgaccgtg
    22441 catcgggtta gcacccggtc cgaacggcgc gccagcacga cgaccgtctg gggtgttacc
    22501 agttttctta ccatacacaa cgttagaagt gatggtcaga acagactgag tcgggatagc
    22561 gtcacggtag gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca ggtcaacagc
    22621 caggtcatct acacgcggat cgttgttacc aaactgcggg tattcgcctt cgatttcgaa
    22681 gtcgatagcc agaccgtctt catcacgaat cggtttaact ttcgcatatt tgattgcaga
    22741 cagggagtca gcagcaacgg acagaccagc gataccacac gccatggtgc ggataacgtc
    22801 acggtcgtgc agcgccatca gagaggcttc gtagctgtac ttgtcgtgca tgtagtggat
    22861 gatgttcagt gcagtgatgt actgtttagc cagccagtcc atgaagtgat ccatgcgctc
    22921 catcacttca tcatagttca ggacatcgcc tttgatcggt tcagacttcg gaccaacctg
    22981 cattttcagt ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt tcgccaggtt
    23041 tgcacgcgca ccgaagaact gcatttgttt accaacgatc atcgggctta cgcagcaagc
    23101 aatagcgtag tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct catactgcag
    23161 agaagaggtg tcgatggaca ctttagcggc gaatttcttg aagttcagcg gcagtttttc
    23221 agaccacaga atggtcatgt tcggttccgg agacggaccc atggtgtaca gggtgttcag
    23281 gaaacggaag ctgtttttgg taaccagggt acgaccgtcg aggcccatac caccgataga
    23341 ttcggttgcc cagatcgggt cgccagagaa cagttcatcg tattccggag tacgcaggaa
    23401 gcgaaccata cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt
    23461 gatcttgcca gctttcaggt cacgttcgat gtacacatcc aggaaggtgg aggtacgacc
    23521 gaaggacatt gcagcaccgt tctgagactt aacagcagcc aggtagccga agtaagtcca
    23581 ctggatagct tcctgagcgt tggtagccgg accagagatg tcgtagccgt atttcgcagc
    23641 catttctttc atctgaccca gagcgcggtg ctgttcagcg atttcttcgc gcagacggat
    23701 agtctgttcc aggtttacgc cgttttccag atcagcctgc agagaggtga actgtgcaag
    23761 tttgtctttc atcaggtagt cgataccgta cagcgcaacg cgacggtagt caccgatgat
    23821 acgaccacgg ccataagcat ctggcagacc ggtcagaaca ccggatttac ggcaacgcag
    23881 gatgtccgga gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt attcagtgaa
    23941 gatttttttg atcatcgggt ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat
    24001 tttgatacca ccgaacggga taagagcacg tttcagcgga gcttcagtct gcagaccaac
    24061 gattttctca agctgcttgt tgatgtaacc agcgtcgtga gaggtgatgg tggaagcaac
    24121 agcggtgtca aagtcaactg gcgcgtgagt gcggttttcc agtttaacgc cttccattac
    24181 tttgtcccac agggtggtgg tcgcttcagt agcgccagcc aggaaggact cgtcaccctc
    24241 gtacggagtg tagtttttct gaatgaagtc acggacgttt acttcattct gccagtcacc
    24301 tttggtaaaa ccttcccagg ctgtggctaa cttttcatta agctcggaca tgtaacacct
    24361 accttcttaa gtggattttt tatttactgc gtacttcgac aaccattaat ggtgatcgtt
    24421 ttcacgcagg taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc cgataatatt
    24481 accgatcgta accggaatca ggttatcagt gatgaaattc atcacggtca ggtgagaaaa
    24541 attttccggt gcagaaccga cagcggtcca gaattccgga gatgcgaagt cgcggattac
    24601 aatacccatc gggatcataa acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa
    24661 catcgcgacc ggcagcacca taataaacgc tttgtccatc aggctgcggc cagaataact
    24721 catccatact gccagacata ccatcaggtt cgccaggata ccaagacaga cggcctcaat
    24781 aaaagtatgg tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc attgaccatt
    24841 tgcggtcata tactcgccgg aaagccacat taaaagtaca aacagcagtg cgccgaccag
    24901 gttgccaaaa taaacattta gccagttttt cgccaactga ccccaggtga tgcgcccact
    24961 cgccttggca acaacaatca acactgtgga agtaaagaga tcggctccgc agacaacaca
    25021 aagaatcaac cccagagaga agcaaatgcc gccaaccagt tttgccatgc cgaagggcat
    25081 tgtacctgtg ccagtggttg ctgtgatata gaagacgaat gcgattgaga tgaaaacacc
    25141 ggcggtaatc gccagataga aagtcttaag cggatgtttc gttgctttat agacacctgc
    25201 ctcttcggcc actttggcca ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt
    25261 cacactaact ctctctttat taagtcggcg acgagatact aacaaagcat tatagatgag
    25321 aaattgatat agatcatatc tcgcctggct tataggcccg taactcgcat ggtttttatg
    25381 caaatacgga gtaaatattt gattatccaa ataaaaataa attttaaaaa ttaacaaatg
    25441 agttgaattt tttccgcatc ctccgctaaa acagttaa
//
LOCUS       DAZFRE010000076        25001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFRE010000076 DAZFRE010000000
VERSION     DAZFRE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.076"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            85..930
                     /locus_tag="ACHKUI_003416"
     CDS             85..930
                     /locus_tag="ACHKUI_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HFO6599557.1"
                     /translation="MEKKFFIDWNNEIPQEEFINNILADEDLVKIRSLVISRWGECYE
                     ESCQPIIDMFAEHAERFAHLESLFIGDMESEECEISWIKQGDYSRLYAALPNLKKLTI
                     KGTQDLELGEIAHDKLEHLEIISGGTPCNVFASLQNSQLPALKTLIVYIGVEDYGFDT
                     SLDIVMKLASKSLFPSLTHLGLVNSEEQNEIVRRVLESDILPQLEVLALSCGTLTDEG
                     AEHLLKHADRLSHLKLLDLYHHYLTTAMQKKLQKTLTCTVELSEALTPEVYDDEVYMN
                     PMFTE"
     gene            990..1976
                     /locus_tag="ACHKUI_003417"
     CDS             990..1976
                     /locus_tag="ACHKUI_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HFO6599558.1"
                     /translation="MITLIGHPESKRTFYFCQAASRLQEHINVVNYDQALTLPLNKSM
                     VKIDPPLHQETDIRLINTVSQNYITFLQKMANRREIRFINSPESIIHLLDKRHCKQRL
                     IEHAIPTPPILADDIRSYDQLIACMRSEKVYRVFIKPNFGSGAAGVLALSLHPGDNRQ
                     VLYCAIQVAGNRLVNSKHIIRYENSDDITLIVNTILQQENIIERWLAKARVGNKSYDL
                     HIVYVNGEIVWRVVRTSSQPITNLHLQNEAIRFADLNLPAEKVAEIDTLCLNAMKLYP
                     DVHIAGLDVLLTQNLTPYIIEINGQGDLIYQDTCEQNIIYQRQIQVMREHHV"
     gene            1969..2841
                     /locus_tag="ACHKUI_003418"
     CDS             1969..2841
                     /locus_tag="ACHKUI_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HFO6599559.1"
                     /translation="MYNVTDEVPENTRTAIHSCDGEINMNDLIAREANVLFVVLDSLR
                     FDIAVQEQEAGNTPNINRYGRWIKCEAAGNFTWPSHHSMFSGFMPKPLEAAPGHSMLF
                     FPKDIGLGRLGPKNAFAFSEETWIKGLENKGYRTICVGGVSFFNNRSGMGKVFPAMFK
                     ESYWYPNFSCTVKESMDNQLTLINKKVNAEHPLMMYINIDTIHYPNHFYIENAKPGDT
                     VETHAAALHYIDARIEKLFDIFRQTGRETLVILCSDHGTCYGEDGKYFHSFNHPIVNT
                     VPYFHFVLDGKTHE"
     gene            2834..4072
                     /locus_tag="ACHKUI_003419"
     CDS             2834..4072
                     /locus_tag="ACHKUI_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HFO6599560.1"
                     /translation="MNNESIYQQYMYSYPHKTAYRELNNIAFADVKSRIYEHKTHLYI
                     HMPFCQSRCGFCNLFTCTGADNAFIDNYIEAIIVQARQMQLAPVDWASFTIGGGTPLL
                     LNVGQLSRLFRGVFDELQVDAQIFKAIETSPSDTTAEKVALLRQLAVNRVSIGVQSFN
                     DLELATLHRRHSTKNAHRALELLRTGNFPILNIDIIYGIPGQTAASLLASLNQALDYQ
                     PDELFLYPLYNMPRQPDMYSLYIEARDRLLHEGYTQTSMRRFVRVNNHSAVESCGFEN
                     SLALGAGGRSYLGNLHYCTPWSQNQPASRQIVADFIARQDKTLINHGYVLSQAEMKRR
                     FVIKNLFFYKGLSLRDYQRQFSSEVLSDFPLLGDFIKQDYCHQQDNRIQLTAQGMALS
                     DQLGPLFISPEVRRLETHKR"
     gene            4069..4923
                     /locus_tag="ACHKUI_003420"
     CDS             4069..4923
                     /locus_tag="ACHKUI_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HFO6599561.1"
                     /translation="MTLKQIQTLIYRGEIKSCNYRCGYCPFHKQDRNDKQQDEAALWR
                     MVEHLPALGSPLNVLITPYGEALRYRYYMAAMAVISRYSHVQAVGIQTNASFNLSALI
                     ATFQHHLGDLAKLRLWCSFHPSQVSKEVFLKQILAIKDAGIACCVGAVASVTDYDAFQ
                     WLRAHLPADIYFWLNANERDKRPTSDERNSQFQRLDPLYHLETSLWPANVAFCLGGKK
                     SVFMNAEGDLFACNISKIKIGNLYQQQLSSPACMARHCHCYLAYHLRQDIPALSQFDA
                     ERCFRIFP"
     gene            4923..5621
                     /locus_tag="ACHKUI_003421"
     CDS             4923..5621
                     /locus_tag="ACHKUI_003421"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFO6599562.1"
                     /translation="MICRLIALDLDGTLLNSKKMISPASVSALQSAQQVGVKIILATG
                     RSHSEALPYYQQLQLTGPMICCNGSYLYHPRQRQILRPLPLDIHKVERLRRWFSQQAL
                     QPHFYTYDDFVLQISLVHRKASLLRQAEEFARQELSLACSWSWHHQLDITQAGCEKGK
                     SLAWYAQQQNIALDEIIAFGDNDNDAGMLQMVGKGIAMGNGSFLAKASSNCVIGHNNT
                     DAIADFLYAQGFSS"
     gene            complement(5696..7519)
                     /gene="typA"
                     /locus_tag="ACHKUI_003422"
     CDS             complement(5696..7519)
                     /gene="typA"
                     /locus_tag="ACHKUI_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6599563.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            7892..9301
                     /gene="glnA"
                     /locus_tag="ACHKUI_003423"
     CDS             7892..9301
                     /gene="glnA"
                     /locus_tag="ACHKUI_003423"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6599564.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            9475..10524
                     /gene="glnL"
                     /locus_tag="ACHKUI_003424"
     CDS             9475..10524
                     /gene="glnL"
                     /locus_tag="ACHKUI_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6599565.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DLSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            10536..11945
                     /gene="glnG"
                     /locus_tag="ACHKUI_003425"
     CDS             10536..11945
                     /gene="glnG"
                     /locus_tag="ACHKUI_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6599566.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            12057..12167
                     /locus_tag="ACHKUI_003426"
     CDS             12057..12167
                     /locus_tag="ACHKUI_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6599567.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(12400..13773)
                     /gene="hemN"
                     /locus_tag="ACHKUI_003427"
     CDS             complement(12400..13773)
                     /gene="hemN"
                     /locus_tag="ACHKUI_003427"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6599568.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(13962..14471)
                     /gene="yihI"
                     /locus_tag="ACHKUI_003428"
     CDS             complement(13962..14471)
                     /gene="yihI"
                     /locus_tag="ACHKUI_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6599569.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(14590..14756)
                     /locus_tag="ACHKUI_003429"
                     /pseudo
     CDS             complement(14590..14756)
                     /locus_tag="ACHKUI_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15053..15685
                     /gene="yihA"
                     /locus_tag="ACHKUI_003430"
     CDS             15053..15685
                     /gene="yihA"
                     /locus_tag="ACHKUI_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6599570.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(16067..18853)
                     /gene="polA"
                     /locus_tag="ACHKUI_003431"
     CDS             complement(16067..18853)
                     /gene="polA"
                     /locus_tag="ACHKUI_003431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6599571.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            19217..20149
                     /locus_tag="ACHKUI_003432"
     CDS             19217..20149
                     /locus_tag="ACHKUI_003432"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6599572.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(20190..21620)
                     /locus_tag="ACHKUI_003433"
     CDS             complement(20190..21620)
                     /locus_tag="ACHKUI_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6599573.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMMLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFNMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVFFNGQEMSEEEFMSRAGRFVH"
     gene            complement(21775..22401)
                     /gene="dsbA"
                     /locus_tag="ACHKUI_003434"
     CDS             complement(21775..22401)
                     /gene="dsbA"
                     /locus_tag="ACHKUI_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6599574.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKQVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(22418..23404)
                     /gene="srkA"
                     /locus_tag="ACHKUI_003435"
     CDS             complement(22418..23404)
                     /gene="srkA"
                     /locus_tag="ACHKUI_003435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6599575.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTVTFIEQAKVLQEPPLQLTPMY"
     gene            complement(23481..23750)
                     /locus_tag="ACHKUI_003436"
     CDS             complement(23481..23750)
                     /locus_tag="ACHKUI_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6599576.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            23820..24404
                     /gene="mobA"
                     /locus_tag="ACHKUI_003437"
     CDS             23820..24404
                     /gene="mobA"
                     /locus_tag="ACHKUI_003437"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6599577.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQESGEWFLFCPCDT
                     PYIPHDLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            24386..24913
                     /gene="mobB"
                     /locus_tag="ACHKUI_003438"
     CDS             24386..24913
                     /gene="mobB"
                     /locus_tag="ACHKUI_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6599578.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
BASE COUNT         6187 a         6189 c         6072 g         6553 t
ORIGIN      
        1 taaaatattc ctcatcctct ctatcaaagc cattgactac ccggtaagat cttttccact
       61 gcaacaccca caaggaatga gaaaatggaa aagaaatttt ttatagattg gaataatgag
      121 atcccgcaag aagaatttat taataatatt ttagctgatg aagacttggt aaaaattcgc
      181 tctttggtca ttagtcgttg gggggaatgc tacgaggaaa gttgtcaacc gataatcgac
      241 atgtttgctg aacatgcaga acgctttgct catcttgagt ccctgtttat cggcgatatg
      301 gaaagcgaag agtgtgaaat ttcgtggata aaacaaggcg attattctcg tctgtatgcc
      361 gcactgccga acctcaaaaa gctgacgatt aaaggtactc aagacctgga actgggagaa
      421 atcgcccacg acaaactgga gcacctggaa atcatctctg gcggtacacc gtgtaatgtg
      481 tttgcgtcat tgcaaaactc tcaactgcct gcgttaaaaa cacttatcgt ttatattggc
      541 gttgaggatt atggttttga tacatcgctg gatatcgtga tgaaactggc gtcgaaatcc
      601 ctcttcccaa gcctgacgca tcttggtctg gtgaacagcg aagagcaaaa cgaaatcgtc
      661 cgccgcgtgc tggaaagcga cattctgccg caactggaag tgttggcact ctcctgcggt
      721 acgcttaccg acgaaggtgc agagcatctt ctcaaacatg ccgatcgcct tagccatcta
      781 aagttgctgg atttgtatca tcattacctg acaacagcga tgcagaaaaa actgcaaaaa
      841 acactgacat gtactgttga attatcagaa gcgctaacgc ctgaagtcta tgatgacgaa
      901 gtttatatga atccgatgtt tactgaataa ataacatttt tgggagagag tcatctctcc
      961 cattatttat cacagaaaag gaattcttcg tgattacgct aattggtcat cctgaaagca
     1021 agcgcacatt ttatttttgc caggcagcgt cacgcttaca ggaacatatc aacgtggtga
     1081 actatgatca agcgcttacc ctgccgctaa ataaaagcat ggtaaaaatc gatcccccct
     1141 tacatcagga aacagatatt cgcttaatta atacggttag ccaaaactat attacttttt
     1201 tacaaaaaat ggctaaccga agggaaatcc gttttattaa ctcaccagaa agtattattc
     1261 atcttctgga taaacgccac tgtaaacagc gattaataga acacgcgatc ccgacaccgc
     1321 cgatattagc ggatgatatc cgtagctacg atcaactgat cgcctgtatg cgcagcgaaa
     1381 aggtttatcg cgtgtttatt aagccgaatt tcggatcagg ggccgccggg gtgctggctt
     1441 tgagtctaca tcccggcgat aaccgtcagg tgctttattg cgccatccag gttgcaggta
     1501 acaggctggt gaacagtaaa cacattatcc gctatgaaaa cagcgatgat attacgttga
     1561 ttgtgaatac tattttacaa caagaaaata ttatcgaacg ctggctggca aaagccagag
     1621 tgggcaataa aagctacgat ttacacatcg tgtatgttaa tggcgaaatc gtctggcggg
     1681 ttgtcagaac atcatcacag ccgatcacca atttgcatct gcaaaatgaa gctatccgtt
     1741 ttgccgatct taatttaccc gccgaaaaag tggctgaaat tgacacgctt tgtctcaacg
     1801 ccatgaaact ctatccggac gtacacattg ccgggcttga cgtattactc acgcaaaatt
     1861 taacacccta tattatcgaa attaacggcc agggcgatct tatttatcag gacacctgcg
     1921 aacaaaacat aatttatcaa cgccagattc aggtaatgag agaacaccat gtataacgta
     1981 acagacgaag taccggaaaa tacccgcacg gcaatccatt cctgcgatgg cgaaatcaat
     2041 atgaatgatt tgatagccag ggaggcgaat gttctgtttg tagtgcttga ttccctgcgt
     2101 tttgatattg cggttcagga acaagaggcg ggcaatacgc cgaacattaa ccgctatggt
     2161 cgctggatca agtgcgaagc cgctggcaat tttacctggc cttcgcatca ttcgatgttc
     2221 tctggtttta tgcctaagcc gctggaagcg gcaccgggtc attcaatgct ctttttcccg
     2281 aaagatattg gtttagggcg tttagggcca aaaaatgcgt tcgcattcag cgaagagacg
     2341 tggattaaag ggctggaaaa caaaggttac cgcactatct gcgtgggtgg cgtctccttt
     2401 tttaataacc gttcagggat ggggaaagtg ttcccggcga tgtttaaaga gagttactgg
     2461 tatcctaatt tctcctgcac ggtaaaagag agcatggata atcaactcac cttaattaat
     2521 aaaaaggtaa atgctgaaca tccgctgatg atgtatatca atatcgacac cattcattat
     2581 cccaatcatt tttatattga aaatgccaag cctggcgata cggtagaaac tcacgccgct
     2641 gccctgcatt atattgatgc gagaatcgaa aaacttttcg atatctttcg gcaaaccggg
     2701 cgggagacgt tagttattct ctgctccgat catggtacct gctacgggga agacggtaaa
     2761 tatttccaca gttttaacca tccgattgtc aataccgtac cctattttca ctttgtgtta
     2821 gacggcaaaa cccatgaata atgaatcgat ttatcagcaa tatatgtaca gttacccgca
     2881 taagaccgct tatcgggaat taaacaatat tgcttttgca gatgtgaagt cgcgtattta
     2941 cgagcataaa acacatctct atattcatat gcctttttgc cagagccgct gcgggttttg
     3001 taatcttttt acctgcacgg gcgcagacaa tgcttttatc gacaactaca tagaggcgat
     3061 tatcgtccag gcccggcaaa tgcaacttgc gcctgtagac tgggccagtt ttaccattgg
     3121 cggcggtacg ccactgctgc ttaatgtcgg gcaactgtcg cgattgtttc gcggggtgtt
     3181 tgacgaatta caggtcgatg cgcagatttt taaggccatt gaaacatcgc cttccgatac
     3241 cactgccgaa aaagtcgcgt tgttgcgcca gcttgcggta aaccgtgtca gtattggtgt
     3301 tcagagtttt aacgatctgg aacttgccac attacaccgc cgccatagca ctaaaaatgc
     3361 ccatcgggcg ctggagctac tgcgcacggg taatttcccg atactcaata ttgatattat
     3421 ttacggtatt cccggtcaaa cagccgcaag tttattggcg tcgcttaatc aggcattaga
     3481 ttatcaacct gacgaactgt ttctgtaccc actgtataac atgcctcgcc agccggatat
     3541 gtatagcctc tatattgaag cccgcgatcg cttattgcat gaaggttata cgcaaacgtc
     3601 aatgcgccgt tttgtgcggg tgaataatca ttcggcggtt gaaagttgtg gctttgaaaa
     3661 ttcgctggcg cttggcgcgg gcgggcgcag ttaccttggc aatttacatt actgcacgcc
     3721 gtggtcgcaa aaccagcctg catcgcgaca aattgtcgcg gattttatcg cccgacaaga
     3781 taaaaccctt attaaccacg gctatgtgct ttcgcaagca gagatgaagc ggcgttttgt
     3841 tatcaagaat ctctttttct ataaagggct ttcgttacgt gattatcagc gccagttctc
     3901 ctctgaggta ttaagcgatt ttcccctgct gggcgatttt atcaaacaag attattgcca
     3961 ccagcaggat aatcgcattc agctcaccgc gcagggtatg gcgctttcgg accagcttgg
     4021 cccgctgttt atttccccgg aagttcgcag gctggagacg cataaacgat gacgctcaag
     4081 cagatccaga cgctgattta tcgcggcgag ataaagtcgt gtaattatcg ctgtggctat
     4141 tgtccgtttc ataaacaaga ccgcaatgat aagcagcagg atgaagcggc attatggcgc
     4201 atggttgagc atttaccggc gctgggttca ccgttaaatg tgttgattac gccctacggc
     4261 gaagcattgc gttatcggta ttatatggcg gcgatggcgg taatcagccg ttattcccat
     4321 gtgcaagcgg tgggtattca gaccaatgcc tcgtttaatt tatctgcgtt aattgcgact
     4381 tttcaacatc atctgggcga tctggcaaag ctgcggctgt ggtgttcatt tcatccttcc
     4441 caggtcagta aagaagtttt ccttaaacag atactggcaa ttaaggacgc agggattgcc
     4501 tgttgtgtgg gggccgttgc cagcgtaacc gactatgacg cattccagtg gttacgcgcg
     4561 catcttcctg ccgatatcta tttctggcta aacgccaatg agcgcgataa aaggcccacc
     4621 agcgacgaaa gaaatagcca gtttcagagg ttggacccgc tttatcatct ggaaaccagc
     4681 ctctggcctg ccaatgttgc gttttgtctc ggcggcaaaa agagcgtgtt tatgaatgcc
     4741 gaaggcgatc tcttcgcctg taatatcagc aaaattaaaa tcggcaattt atatcagcag
     4801 caactttcat ctccagcctg tatggccagg cactgccact gttatctggc ttatcatctg
     4861 cgtcaggata tcccggccct ctcacaattt gatgctgaac gctgttttcg gatcttccct
     4921 taatgatctg ccggttaatc gcgctcgatc tagacggcac attgctgaac agcaagaaga
     4981 tgatttcccc tgcgtcggtt tcggcgctgc aaagcgcaca acaggtaggt gtgaagatta
     5041 ttctcgccac cggacgttcg cacagcgaag ccctgccgta ttaccagcaa ttgcagttaa
     5101 ccgggccgat gatttgctgt aacggcagct atctttatca tccgcgccag cggcaaatat
     5161 tgcgtccgct gccgcttgat atccacaagg tggaacggct acgacgctgg ttttcacaac
     5221 aggcattaca gccacatttt tatacgtatg atgattttgt tttgcagatt agcctggtac
     5281 atcgcaaggc ttcgttattg cgccaggctg aggaatttgc ccggcaagaa ttatcgctgg
     5341 cctgtagctg gtcctggcat catcaactgg atattacaca ggcggggtgt gagaaaggca
     5401 aaagtctggc ctggtatgca cagcagcaaa atatcgctct ggatgaaatc atcgcctttg
     5461 gcgataacga taatgatgcg gggatgttgc agatggtagg gaaaggtatt gcgatgggca
     5521 atggtagctt tctggcaaaa gccagctcta actgcgttat cggtcacaac aacactgacg
     5581 cgattgcgga ttttctttat gcgcaggggt tcagttcatg atgcctctta tcaggtctac
     5641 aaaagcaaaa aaacctgcca tcgctggcag gtttttttat gactaaaaaa cgaaattaat
     5701 cgtctttcgg tgcgcggttg gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa
     5761 tacggataga ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt
     5821 gttccagagt catgcggata ggcggaacca gaacaacggc ttcgtcagta ccggaagcac
     5881 gcatgttggt cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat
     5941 gaataccgat aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccac
     6001 ggtcctgcag accgaacagc gcgaacgcga ccgctttacc ctgaccgtta gagatcagta
     6061 caccgttctg acgctgaccc acttcgcccg gacgtacgtc gtcgtagtgg ctgaaggtgg
     6121 agtacagcag accagtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac
     6181 cacggcttgg gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt
     6241 tcaggtcgcc tttacgttcg cccagcgcct gcatcacaga accctgatgc tgttcttcaa
     6301 cgtccagcgt cacgttttca tacggctctt gtttacgacc gtcgatttca cggaagatta
     6361 ctttcggacg ggataccgcc agttcgaaac cttcacgacg catgttttcg atcagaacag
     6421 acaggtgcag ttcgccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta
     6481 cgcgcagcgc aacgttgtgt accagttctt tgttcagacg atccaggatc tgacgagacg
     6541 ttacgaactt accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa
     6601 cggtcggctc atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg
     6661 tgtcagaaat gttcagttcg ccaaggcccg tgatcgccac gatatcgcca gcttccgcca
     6721 gatcggtttc gatacgttcc agaccgaggt ggcccagcac tttaccgact ttcgcgttgc
     6781 gggttttgcc ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccgcgct
     6841 tgatgcggcc aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca
     6901 tctggaacgg accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt
     6961 acagcggggt catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg
     7021 cagaagcgta aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca
     7081 gatcgaatac ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga
     7141 taacaacaat cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg
     7201 gcatcgggcc gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac
     7261 gttcaacttc accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat
     7321 cattccattt gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat
     7381 cgttggagtc catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt
     7441 ggagcagctt gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat
     7501 tacgcaattt ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac
     7561 ggattaatcg cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt
     7621 ccctttgtga tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata
     7681 tggtgcttaa tgttcccatt gaagcactat attggtgcaa cattcacatc atggtgcagc
     7741 ccttttgcac gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag
     7801 atttcgcttt atctttttta cggcgacacg gcccatttaa ttgcagattt cgttaccacg
     7861 acgacaatga ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct
     7921 gaacgagcac gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca
     7981 gcacgtcact atccctgctc atcaggtgaa tgccgaattc ttcgaagaag gcaaaatgtt
     8041 tgacggctcc tcgattggcg gctggaaagg cattaacgaa tccgatatgg tgctgatgcc
     8101 agacgcatcc accgcagtga ttgacccgtt cttcgccgat tctaccctga ttatccgttg
     8161 cgacatcctt gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa
     8221 gcgcgctgaa gattacctgc gttctactgg cattgccgac accgtactgt tcgggccaga
     8281 acctgaattc ttcctgttcg atgacatccg tttcggatcg tctatctccg gttcccacgt
     8341 tgctatcgac gatatcgaag gtgcatggaa ctcctccacc caatatgaag gtggtaacaa
     8401 aggtcaccgt ccggcagtga aaggcggtta cttcccggtt ccgccggtag actcagctca
     8461 ggatattcgt tctgaaatgt gtctggtgat ggaacagatg ggtctggtag ttgaagctca
     8521 tcaccacgaa gtcgctaccg caggtcagaa cgaagtggct acccgcttca ataccatgac
     8581 caaaaaagct gacgaaattc agatctacaa atatgtcgtg cacaacgtgg cgcaccgttt
     8641 cggtaagacc gcgaccttta tgccaaaacc gatgttcggt gataacggtt ccggtatgca
     8701 ctgccacatg tcgctgtcta aaaacggcgt caacctgttt gctggcgaca aatacgcagg
     8761 tctgtctgaa caggcactgt actacattgg cggtgtaatc aaacacgcga aagcgattaa
     8821 cgccctggca aacccgacca ccaactctta caagcgtctg gtcccgggct acgaagcacc
     8881 ggtaatgctg gcttactctg cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc
     8941 ttctccgaaa gcacgtcgta tcgaagtacg tttcccggac ccggcggcta acccgtacct
     9001 gtgctttgct gccctgctga tggccggtct tgatggcatc aagaacaaga tccatccggg
     9061 cgaagccatg gacaaaaacc tgtatgacct gccgccagaa gaagcgaaag agatcccaca
     9121 ggttgcaggc tctctggaag aagcactgaa cgaactggat ctggaccgcg agttcctgaa
     9181 agctggtggc gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga
     9241 agatgaccgc gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta
     9301 atagttgaag ttgtactacc cggcgcaaca acgccgggat ttagttgccg tggaaacttt
     9361 cagcccatct ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat
     9421 ccgtaaaaag ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca
     9481 acaggcacgc agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta
     9541 atcgatgaca atctggcgat ccattacgcc aaccccgccg cgcaacaact gctcgcccaa
     9601 agctcccgca aattgtttgg tacgccgtta ccggaactgt tgagctactt ctcattaaat
     9661 atcgagctga tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg
     9721 ctggtcatcg acgggcgctc gcatatcctt tctgtgacgg ctcagcgtat gccggacggc
     9781 atgatcctgc tggagatggc accgatggat aaccagcgcc gcttaagtca ggaacagcta
     9841 cagcacgccc agcaggttgc tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa
     9901 aatccgcttg gcggtttacg tggcgcggcg cagctgctca gcaaagcatt acctgacctg
     9961 tcactactcg aatataccaa agtgattatc gaacaggcgg accggctgcg aaatctggtc
    10021 gaccgtctgt tggggccgca gctgcccggt acgcgcatta ccgaaagtat tcacaaagtg
    10081 gctgaacgcg tggtgacgct ggtgtcgatg gaactgccga acaacgtgcg gttgattcgg
    10141 gattacgacc ccagcctgcc ggaactggcg cacgacccgg atcaaattga acaggtcttg
    10201 ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg agggtggtga aatcattcta
    10261 cgtacccgca cagcgtttca actgacctta cacggcgagc gctatcggct ggcggcgcgg
    10321 attgatgtgg aagataacgg gccgggcatt ccgcctcatt tgcaggatac gctgttttac
    10381 ccgatggtca gcggccgcga aggtggcacc gggcttggct tatccatcgc ccgtaatttg
    10441 attgatcagc attcaggcaa aattgaattt accagttggc caggtcatac cgagttctcg
    10501 gtttacctgc ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag
    10561 tcgatgacga tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa
    10621 cctgtacgac atttgagaac ggcgcggaag tactggaggc gctggcgagc aaaacgccgg
    10681 atgtgctgct ttcagatatc cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga
    10741 ttaaacagcg ccatccgatg cttccggtca tcattatgac cgcacattcc gatctggatg
    10801 ctgccgtcag cgcctatcaa caaggggcat ttgattatct gcccaaaccg tttgatatcg
    10861 acgaagccgt ggcgctggtt gagcgcgcca tcagtcatta ccaggaacag cagcagccgc
    10921 gtaatgttca gcttaacggc ccaacgaccg atatcatcgg tgaagcgcca gccatgcagg
    10981 acgtgttccg gattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg
    11041 aatccggcac cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgcgagcca
    11101 aagcgccgtt tatcgcgctc aatatggcgg ctatcccgaa ggatttgatc gaatccgaac
    11161 tgtttggtca cgagaaaggc gcatttaccg gcgcgaatac cattcgtcag gggcgttttg
    11221 aacaggctga tggcggtaca ttatttctcg atgaaattgg cgatatgccg ctggatgtgc
    11281 agacgcgttt gctgcgcgtg ctggcagacg gtcagtttta tcgcgttggc ggctatgcgc
    11341 cggtgaaagt agatgtgcgg attatcgctg ccactcacca gaatcttgaa cagcgggtgc
    11401 aggaaggcaa gttccgtgag gatctgttcc accgcctgaa cgtcatccgc gttcatctgc
    11461 cgccgctgcg cgaacgtcgg gaagatattc cccgtctggc gcgccatttt ttacaggttg
    11521 ccgcgcgaga gctgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa gccgcgctga
    11581 cgcgtctggc gtggccaggc aacgtgcgcc agctggaaaa cacctgtcgc tggctaacgg
    11641 tgatggccgc cgggcaggaa gtgttgattc aggatttgcc tggcgaactg tttgaatcaa
    11701 acgtaccgga aagcacttca cacatgcagc cggacagttg ggcaacgctg ttagcacagt
    11761 gggcagacag agcgctgcgt tccggtcatc aaaacctgct ttccgaagca cagccagagc
    11821 tggaacggac gttactgacc accgcgctgc gacatacgca ggggcataaa caggaagcgg
    11881 cgcggctact tggctggggt cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg
    11941 agtgattcac ggcttgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg
    12001 ctttactgtt ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc
    12061 tggaatcaat aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg
    12121 ctgttgctgc cgtgctgtgt gccgcgatga ttggtctgtt tagctgaagc tcgttgttta
    12181 cgccggatgc ggagaaagtc gtaggcctga taagacgcaa ccagcgtcgc atcaggcgtt
    12241 gattgccgga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacagat
    12301 tttgcaggcc tgataagcgt agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc
    12361 tttcgttttc tactttgtaa acgaagcgcc attcacaatt taaatcaccc gagagaactg
    12421 ctgcatccgc gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag
    12481 caagcgacct ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt
    12541 tgctaacggg gcgagcagct tgagatcttc cgcaaagtaa tcagcgaaga gcaaatccca
    12601 ctgttgctca ataggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc
    12661 gcggcgaata cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc
    12721 atccacttgc tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat
    12781 catgctgatg gcggaaacgc ccatcccaag cagatcggta tcgccctggg tggtgtagcc
    12841 ctggaagtta cgatgcagca cgccttcacg ctgggccacc gccagctcgt catccggacg
    12901 ggcaaagtga tccataccga taaactgata gcccgattgt gtcaggaagg cgatggtttc
    12961 ctgcaggata tcgagttttt gctgcggact cggcaggtca gcgtctttga ttttgcgctg
    13021 agcagcaaaa atggtcggca aatgcgcgta gttaaagacg ctcagacgat cggggttcag
    13081 ctccgccacg cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta
    13141 aatcaggtcg atgttggtgg aggtaaagcc aatctcacgc gcatggttaa gcagtgcaaa
    13201 gatgaactct tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg
    13261 cacgcccatg ctcagccgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc
    13321 gatttcccgc ggatcgactt cgatcgagat ctccgcatcg gcattgaact ggaagttttc
    13381 gcgcagcagc ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc
    13441 gccccagtgc aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc
    13501 ttgctccagc gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt
    13561 gcaaccgcag aagtaacaaa gcttatggca gaacggaata tgtacgtaga gagataatgg
    13621 acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa
    13681 ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag
    13741 ggccagatcc cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg
    13801 cggctacggc gtaccggcgg atgcccgttt cgagtcatca gccggttgcg cagccacttt
    13861 tgtcgccgcg acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga
    13921 agggttatca gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga
    13981 cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg
    14041 ccgagtttct gcatcaactc atcaatacga tccagtttgg catccaccca ggattgctct
    14101 tcggcgctca gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc
    14161 gtttccagta actccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg
    14221 tgctgcttgg tgactttttc agtcacgccc aatggaatag gggttttact gccaatacgt
    14281 ggatcttttg gtgcgttctg acctttgctg cctgacgtgg tgttaccgcc cgctgcgcgg
    14341 ctgcccggcg catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc
    14401 agctcctcgc gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat
    14461 gatggtttca taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat
    14521 ctaacagaaa gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac
    14581 tcataaccct taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat
    14641 tagccggaac aatccgtgtt gattccattt ccatttaatt acgtctccgg acgtttatct
    14701 tcctgacaat cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt
    14761 cctgttagcg tcctcgctct atgggtgcta ctttacgcct ttgctttaaa taaacaatca
    14821 accgccatca gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata
    14881 tagcttattt gacatacgtc gcagaatttt ctctgctatt tcgcataaag actatcggca
    14941 atgtctcaca aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta
    15001 ttatggttca ggtataatcg ccgcaaagct ttgactgatg gagacgaccg ctttgactaa
    15061 tttgaattat caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc
    15121 cgataccgga attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct
    15181 gaacacgctg actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca
    15241 gcttatcaac ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg
    15301 ttatgcggaa gtcccggaag agatgaagcg caaatggcag cgcgcgctcg gcgagtacct
    15361 cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa
    15421 agatttggat cagcagatga ttgagtgggc ggtagacagc aatatcgccg tgctggtact
    15481 gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg
    15541 tgaagcggtg ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgctgaagaa
    15601 acaaggcgtg gacaagctgc ggcagaaact ggatacctgg tttagcgaga tgcagcctgt
    15661 agaagaaacg caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt
    15721 atccggcccg taaattcaat agattacaat tctaccgttg gcctgataaa catggcgtat
    15781 caggcattac ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg
    15841 gcggctaaaa tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca
    15901 tcttacctct gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcatt
    15961 ttttgtaatt ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac
    16021 agcttatgtt gcttacttac gaaaaaaggg catgttcagg caaatcttag tgcgcctgat
    16081 cccagttttc gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt
    16141 ccatcagttg atgaatctgc ttcgcgacgg catcgacatc atctttatgg acttcaaata
    16201 ccagttcatc gtgtacctgc atgatcatac gaacgcgcgg ttgctcagcc tgtagccacg
    16261 catcaacggc aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt
    16321 taatggctgc acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca
    16381 gatacagacg gcgtccgtcc agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg
    16441 tgcgttccat atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct
    16501 gcgcttcttt acgcggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa
    16561 tcagaccaaa gttgatcgct ttcgcgctac gacgttgctc gctggtgacg gtttccagtg
    16621 gcaaaccaaa cacttctgcc gccgttgcac ggtggatatc ttttccttcg gcgaatgcgg
    16681 tcagcaagcc tttgtcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt
    16741 ccgctgagac aatcacataa tcctctggcg caataaacgc ctggcggata cggcgacctt
    16801 cttcgttacg caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg
    16861 cggttactgc ctggtgataa gaggtatgta cacgcccggt tttcgggttg atcatcaacg
    16921 gcagcttgtc ggtgtaggtc gatttcagct tcgccagacc acgatactcc agaatcactt
    16981 ttggcaacgg atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc
    17041 cacccggagt tttcttcagc ggtttaatgc cctgtttttc aaacagaatg gtttgtaact
    17101 gcttggtgga agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct
    17161 cagccagacg caaggtgagc tcttcagagt gattgtgcag cactttcgga tcgatcttca
    17221 caccgttacg ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga
    17281 cgttcaacgg ccctttgtgt ttttgcagat ccggccacat tttcagatgc aactgcaagg
    17341 tgacatctgc atcttcggcg gcgtaacgtc cggcttcttc gagggcaatc tggttaaagg
    17401 tcagttgatt tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc
    17461 aacgttccgc gaggctgtcc atatcgtgac gtccggcaac gctattgaga atgtaggact
    17521 ccaacatggt atcaaacgca atcccacgta gttcaatgcc gtagttcgcc agaataccgc
    17581 gatcgtattt caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta
    17641 gcagctcgag tgcacgctcg cgagagattt gatcgggcgc atcaagataa tcatgagcaa
    17701 ccggaatata tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag
    17761 agatgttatc aaggctgtca gtttcggtat caaatgcaaa taccggcgct ttttccagct
    17821 tcgcaatcca ctctttcagt gtttcttcat caaggatggt gacgtagttg tcataagaaa
    17881 tcaccgttgc cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcctggctg
    17941 cgggtttcgc ccctttagcc tgtagccatt ttcccgcttc gacatcagca gtccagcgtt
    18001 tgaactcata ctttttgaac agccctaaca actcttccgc cgccggtggc tgcacttcca
    18061 gttgttcaca ggtcagctcc agttcaacgt cggttttaat cgtcgccagc tggtatgaga
    18121 gataagcaac ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc
    18181 tcaacccagc aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca
    18241 gcaatgcctg cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt
    18301 cacccatcag cgccaggaaa tcgatgatca gttctggcgg cacgccgtac ttattcacca
    18361 cctcttccgg tccgaggatg gtattcgtca tggtgttgat aagcgtgata tttggtgtca
    18421 ccagctgcgc catatcttta tcgccagtgc tgatcagcac cggacgcccg gctttttcgg
    18481 cttcgcgcgc cagagtacca ataacgtcgt ccgcttctac gccagaaacc gccagcagcg
    18541 gcagtcccat cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg
    18601 gcattggcgg gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct
    18661 tggcgtcaaa gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca
    18721 tgttgaggac accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa
    18781 acgcgtgata tgcgcgataa aggtaagatg aaccatctac aaggataagt ggattttggg
    18841 ggatctgaac cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac
    18901 aggatgaaaa catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat
    18961 cgctcgcaaa tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt
    19021 attatcatgc tttttacagc atggtgtttg tctttgtttt tcatgcagtt aaaaataaag
    19081 ccatccctaa taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt
    19141 tctccttaag gatcatccgt tatttgggtc gttttctgct aaaatctgcg ccttgcgctt
    19201 ctttagcgca ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc
    19261 tgcgataacc cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt
    19321 cccgatcatc attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa
    19381 ggtttcgcgt ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact
    19441 gcatctgaac ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa
    19501 ctggtatctg cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct
    19561 gtttcgtaag cacattccga tgaataaata tttcctcaag cagcagctgg cctgggtgcc
    19621 ttttcttggc ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc
    19681 ttatttatta cgtcatccgg agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg
    19741 tgaaaagttt cgcctgcatc ccaccactat tgtcaatttc gttgaaggct cccgtttcac
    19801 gcaagaaaaa catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc
    19861 aggcattgca atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac
    19921 gctgtgttat ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac
    19981 gcgaattgtc gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat
    20041 caatgacaaa agctttaagc gccattttca gcaatggctt aactcattgt ggcaggaaaa
    20101 agaccggctt ctgacatcgt tgatgtcatc acagcggcag gaaaagtaag ttttactcgc
    20161 ctctgaatga gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata
    20221 aattcttctt cgctcatctc ctgaccgtta aaaaaaactt tacccggtgt ataacgcagt
    20281 tgcagcgagg cggtattgtt gtcaatcgtg attaactgca acatctgacc taatgtcatc
    20341 atcccgctga tttgtttatc agcccgcttt tgcgcttttt ccgcatccat cccttcagat
    20401 aaattaagct gttttgcggt ttctgtggcg acattaagcg gtaacttcat attaaaattg
    20461 agcgatttaa tatctttgtt tgtggatgat gaagatttgg cggggtcggc aatactgata
    20521 tccagattgg catttagttc gccgagtgcg ttcttccatc ttactggttg tttaatggtc
    20581 ggctcacttt tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc
    20641 agagcaactt catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg
    20701 ttatactgga tgataaattg gcgcaccgct gagggatcaa tagactcagc aatcaatgac
    20761 cactcaccac taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga
    20821 ttgacattga tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca
    20881 tcgatttggg caacgacttc cccctggttg ataatattaa cctgattaaa tttcgactcg
    20941 ctttcaccaa ccggaaattt cttttcttct aatcgtgtca gagaatcaat ggtaaaactt
    21001 ttcgccgtta ctttgttcat tggtgaaaat tgcagaacaa tatcttcgac tttgccttca
    21061 atatccaggt tagacaatga actatcatca ccattaaaag taaactggcc ttcaccaaaa
    21121 gaaaactttc catactctgc aggggcaaca tctaatgtcg tggagaatga gccaccaaac
    21181 gcgaatcgca atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt
    21241 gtcagctcat tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca
    21301 tgcattaaca ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaattttc
    21361 tggcctggct tgatattgag atcgggtgca ccgttatcaa atgtcatttg catttgaaaa
    21421 cgggaattaa aaaagccttt gtcaaaattt ttataactta acatcatatc gtaggcatgt
    21481 tcacctttct ttttagcatc gttaaaacct ttaataaact tttcgacacc aggctgaatt
    21541 tgcgtacctg tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aatggcacct
    21601 gtagctgact tacgtatcat ataaatattc cctgataaaa cagcaaaact ttgaatatcc
    21661 acttatgctg aaataatgtc cagcagcagg acgcattatc agaatgtatt tatatttatt
    21721 aaataataaa aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt
    21781 tttctcggac agatatttca cagtatcagc atactgctga acaaaaacat ccatattgct
    21841 ggtatccata ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc
    21901 acgcaattgc acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa
    21961 gctgttccac gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg
    22021 gatatcagat gctgaacgaa tggtctgggt tttttgtacg ccttcaaaca gcggaactgt
    22081 gactttgtct tccacgccca gcgccatcgc cacagcccat gcctgagtca gctctttgcc
    22141 caggtcaccc cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt
    22201 tttcttcacg ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcaaaa
    22261 gaaagagaaa aactccagca cttgcggcgc gccagcaact tgtttttcca gggtagtgta
    22321 ctgtttacca tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag
    22381 cgccagccaa atctttttca tgatctactc tctccgatta atacataggt gttaattgca
    22441 aagggggttc ttgtagaact tttgcctgtt ctataaaagt caccgtctgt cgtagccagt
    22501 aatcttcccc ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caacgccgca
    22561 ttagccaggc aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt
    22621 cagcggtgtc gaactcgcta aattcttcat aagcctcaat aatagtttcc agttgcatcc
    22681 gctgctcggc tttatcacca ttgagcaaca tccacaaatc ctgaacggct ggaccattac
    22741 gtgcatcatc cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc
    22801 agtctccatg tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcaa
    22861 tcagctcatc tgtcgctttc aggaatgccg ctttcaaccc ggaaggtatc agtgtagcgt
    22921 cctcaaacag cttgcgtggc tcaatgagat attcattcaa accgatggtc gggcgatgga
    22981 taaaaagctg tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca
    23041 tctgatcgat attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat
    23101 aaaatccctg atggttcaat aaagtctgac cattaaaggc cacaggtgct gcgaccggaa
    23161 cttcatcgtt aaccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc
    23221 aacgttcagg gcgataaaat ttgacgacaa aacgtcgacg atcttcgtcc tgaaattgat
    23281 agacacggtt ttcatagctg ttaagcgggg taagaccgga atccacccga atcccatgct
    23341 caaacagagc gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt
    23401 tcatcccatc atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg
    23461 gttgcgccgc ttacaagctt ttactcttta attacgccac gtgcgcgtaa tagtgccgtt
    23521 ttgaaatctt cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca
    23581 cgcattttca ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa
    23641 cctgactctt tcgccagttt ctgcaaaaat tgcagcaggt taaggtctgg ctctttttgc
    23701 caggctggct ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc
    23761 cttactcttg agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg
    23821 tgaatctgat gacgacgata acaggcgttg tgctggcggg cggtaaagcc agacgaatgg
    23881 gcggcgtaga taaaggattg cttgaattaa acggcaaacc attatggcaa catgtcgctg
    23941 acgcgcttat gacgcagctt tctcacgtcg tggttaatgc taatcgtcat caggaaatct
    24001 atcaggtaag cggtctgaaa gtgattgaag attcactggc ggattaccct ggccctctgg
    24061 caggaatgct ttcagtaatg cagcaggaat cgggtgagtg gtttttgttt tgcccgtgcg
    24121 atacgcctta cattccccat gatttagcct cccggcttaa tcatcagcgc aaagatgcgc
    24181 ctgtcgtgtg ggtccacgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg
    24241 caattgagcc tttattactg gaatatctgc aagcaggaga acgccgggtt atggcattta
    24301 tgcgtctggc tggcggtcat gcggttgatt tcagcgatcg taaagaggca tttataaacg
    24361 tgaatacgcc agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt
    24421 tgctgcatgg agtggcaccg gaaaaacaac gctgctgaaa aaattgatcc cggcattatg
    24481 cgccagaggg atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa
    24541 accgggcaaa gatagctatg agctgcgcaa ggctggcgcg gcgcaaacca tcgttgccag
    24601 ccaacagcga tgggccttga tgacagaaac accagacgaa gaagagctgg atctacattt
    24661 tctcgcaagt cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca
    24721 tgaagagatc gcaaagattg tgctgtttcg cgatggagcc ggacatcgac cggaagaatt
    24781 agtgatagac aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc
    24841 gttactggat attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa
    24901 gcaggacgga taagcaagag taggctggag caaacgcgcc agcgtcgcat ccggcattta
    24961 ttccgcacca acgcaaaaaa gcccagtctt ccgactgggc t
//
LOCUS       DAZFRE010000077        24926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFRE010000077 DAZFRE010000000
VERSION     DAZFRE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.077"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            293..484
                     /locus_tag="ACHKUI_003439"
     CDS             293..484
                     /locus_tag="ACHKUI_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6599579.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALATVTSVEETRVVFMREGYPHPCM
                     RPMYNFLGKFKPDPREETE"
     gene            complement(537..770)
                     /locus_tag="ACHKUI_003440"
     CDS             complement(537..770)
                     /locus_tag="ACHKUI_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599580.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLAYNRNRVVEVVTRKL
                     QDSFYINVSWDALNTAYNEHPEWFSGLVSGDEN"
     gene            complement(866..1365)
                     /locus_tag="ACHKUI_003441"
                     /pseudo
     CDS             complement(866..1365)
                     /locus_tag="ACHKUI_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            1716..2834
                     /gene="grpA"
                     /locus_tag="ACHKUI_003442"
     CDS             1716..2834
                     /gene="grpA"
                     /locus_tag="ACHKUI_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system alcohol
                     dehydrogenase GrpA"
                     /protein_id="HFO6599581.1"
                     /translation="MARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTR
                     YLISHMPEASVCIFGEVTPDPDIDILTSGAARFKAFKPDVIIALGGGSSLDAAKGIKV
                     TLEEYFGDHQIDLIAIPTTSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPN
                     LVLTAPRHVAVDTGMDVLTHAIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKD
                     IAARTHMHNASCMAGMAFNSAGLGLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSK
                     RASTETRDRYHRCAQIIGIDHAVPEQAIALMTQEIRQINRHFCIPVTLSELGIDRAKI
                     IELRSALVNSTLADGCTASNPRQVTTHDVEGLIDLITG"
     gene            2974..3660
                     /gene="grpB"
                     /locus_tag="ACHKUI_003443"
     CDS             2974..3660
                     /gene="grpB"
                     /locus_tag="ACHKUI_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system
                     phosphotransacylase GrpB"
                     /protein_id="HFO6599582.1"
                     /translation="MNYTEQQAEWITQQILQELATSKKTAAPAAAPVALNIPVGVSNR
                     HVHLCREDMDILFGYGSTLTRSKALKQPGQYAAEEMVTLRGPKGELNKVRVLGPLRNA
                     TQVEISVADGFALGTKAPVRMSGDLLDSPGIEIIGPKGRVVKHNGMIVAWRHIHISPE
                     EAELHGLHDGMEIDVQTNGPRGGVLSHVVVRVTADAVLELHIDMEEANGVGLRNGDTV
                     RGIKQAARHG"
     gene            3715..4473
                     /gene="grpC"
                     /locus_tag="ACHKUI_003444"
     CDS             3715..4473
                     /gene="grpC"
                     /locus_tag="ACHKUI_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system chaperone GrpC"
                     /protein_id="HFO6599583.1"
                     /translation="MSALLRRVEGGGDLGTANIAIVVLDEDNQPVAGVMYPSNVVKDG
                     VVVDYLTASRVVARLKQILEQKQGCTLERAAIAVPPGISEGNTKVIVNVVELAGFIVT
                     QVLDEPVAAASALNIKNGAVVDVGGGTTGMSILKSGKVTFSADEPTGGSHMKLVIAGA
                     LGLPYAEAEEMKKNGQEAQVFTLVLPVAQKMASLVSGWFATQQVKNIYLVGGASSFSQ
                     FANVFSKQTGKNVIPSAEPLLVTPLGIAMNADQG"
     gene            4457..5182
                     /gene="grpD"
                     /locus_tag="ACHKUI_003445"
     CDS             4457..5182
                     /gene="grpD"
                     /locus_tag="ACHKUI_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system flavoprotein
                     GrpD"
                     /protein_id="HFO6599584.1"
                     /translation="MRTRGKSMDNQTLSQLLDRVIAEVLAQSVAQKNVCVVVTGEDIS
                     SLPETLNCLEALELAGYQLWVNFSHSASQSALKSVCMDALIQRGSRAGFDRSPPHYEV
                     LYLPALSVNSMSKIALGIRDNLACETVFQALQHRKQIIATLHQQCVDSELPMPLLSCL
                     EGYAQVLESYGITLFGKRATHVKSVQGSQSTLTVAGKKRLITQSDIRLLTIGTVLRIE
                     SDTLITPAARDEIQRRNLTVIHG"
     gene            5194..5463
                     /gene="grpE"
                     /locus_tag="ACHKUI_003446"
     CDS             5194..5463
                     /gene="grpE"
                     /locus_tag="ACHKUI_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment vertex
                     protein GrpE"
                     /protein_id="HFO6599585.1"
                     /translation="MYLAKVTGALVSTTKHASLNGSKLLIVARLDENYQPTVHAQVAV
                     DTVGAGNGEIVIITTGSSARMSNNKDHSVIDAAVVGIVDSLELNG"
     gene            5491..5988
                     /gene="grpF"
                     /locus_tag="ACHKUI_003447"
     CDS             5491..5988
                     /gene="grpF"
                     /locus_tag="ACHKUI_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system heme-binding
                     protein GrpF"
                     /protein_id="HFO6599586.1"
                     /translation="MSANEASLDNWINEAIATQLPELRMSLSLEDSELLAKLVREIAQ
                     SSNVSIVFSLVDACGQQRFFFSMDNALLVSHTLAPQKAWTAVALKMPTHELATLVQPG
                     GDLYGLEKEKDICCFGGGFPCWSGRRLLGGIGISGGSVEEDMLIARQAMAQFSVLRYP
                     LTTTR"
     gene            6017..7213
                     /gene="grpG"
                     /locus_tag="ACHKUI_003448"
     CDS             6017..7213
                     /gene="grpG"
                     /locus_tag="ACHKUI_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system propionate kinase
                     GrpG"
                     /protein_id="HFO6599587.1"
                     /translation="MSLACVLVINCGSSSMKFSVIPQDADQPLLSGLAERLGIDHAVI
                     TFKDRDGHKSTVALDDASHQHALKVLFAKLDEQQLLEAINAVGHRVAHGGSDFKRSVL
                     VTDDVIEKVRALSVLAPLHNPANLIGIEAARALLPALPHIAVFDTAFHQTLSPAAYTY
                     AIPLEFQQDYMVRRYGFHGTSHRYIAAEALASLDLDPADHGIVIAHLGNGSSLCAVQN
                     GTSIDTSMGMTPLEGLVMGTRCGDLDFGVVAYLAKRTGQTFDTLYKMLNNQSGLMGIS
                     GLSSDCRTLQQARDEGHERAALAIDVLVHRLARHIGGHVTSLKRFDALIFTGGIGENS
                     ALIRELTVQRLQALGLELDKERNQQLFGGRSGLISKTDTTKIAVIPTNEEKMIAIDAA
                     EIARTA"
     gene            7498..7779
                     /gene="grpH"
                     /locus_tag="ACHKUI_003449"
     CDS             7498..7779
                     /gene="grpH"
                     /locus_tag="ACHKUI_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system shell hexameric
                     protein GrpH"
                     /protein_id="HFO6599588.1"
                     /translation="MQQEALGMVETKGLVSAIEAADTMVKSANVTLVGYEKIGSGLVT
                     VMVRGDVGAVKAATDAGSAAASKVGELVSVHVIPRPHIEVEKILPKAIG"
     gene            7801..8586
                     /gene="grpI"
                     /locus_tag="ACHKUI_003450"
     CDS             7801..8586
                     /gene="grpI"
                     /locus_tag="ACHKUI_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     GrpI"
                     /protein_id="HFO6599589.1"
                     /translation="MRDNLVEQIISDVMNKQTDISPEAKTAANFAGCGITEFVGTALG
                     HTIGLVIANVDNQLHEVMNIDKKYRSIGILGARTGAGPQIFAADEAIKATNSEIISIE
                     LARDTEGGGGHGCLIIFGASDVSDVRRAVEVALSEIERTMGDVYGSSAGHLEFQYTAR
                     ASYALNKALGAPIGKSFGMTCASPAAIGLVIADAAAKSAVIDPVGYASPSQGTSFSNE
                     VIFTFSGDSGAVRQAVIAAREVGLQLLSTLDPVEIKSTTTPYI"
     gene            8647..10164
                     /gene="grpJ"
                     /locus_tag="ACHKUI_003451"
     CDS             8647..10164
                     /gene="grpJ"
                     /locus_tag="ACHKUI_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system aldehyde
                     dehydrogenase GrpJ"
                     /protein_id="HFO6599590.1"
                     /translation="MNDIEIAQAVSTILSKFTKATPDEAPATSEAARVDGLDEIVAKA
                     LAQHSSVRDASAISQVAKVANASTGAFDTMDEAISAAVLAQVQYRHCSMQDRASFING
                     IRDVFLQEDVLCALSRMAVEETGMGNYEDKLIKNRVAALKTPGIEDLTTSAVSGDGGL
                     TLIEYSAFGVIGSITPTTNPTETIINNSIGMLAAGNTVVFSPHPRSRKVSLYAVELIN
                     NKLAQLGAPANMVVTVTKPSIDNTNVLINDPRINMLVATGGPAIVKTVMSSGKKAIGA
                     GAGNPPAVVDETADIEKAARDIIKGCSFDNNLPCVAEKEVIVVNQVADYLIHCMKKSG
                     AYLLCDKKLSQQLQSLVLNEKGTGPNTAFVGKDARYILQQLGIQVGDDIKVILIEAEK
                     THPFVVHELMMPVLPVVRVDNVDEAIELAVKVEHGNRHTAVMHSTNVEKLTKMARLIQ
                     TTIFVKNGPSYAGLGVGGEGHATFTIAGPTGEGLTSARSFARRRRCVMVEALNIR"
     gene            10188..10487
                     /gene="grpK"
                     /locus_tag="ACHKUI_003452"
     CDS             10188..10487
                     /gene="grpK"
                     /locus_tag="ACHKUI_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system shell hexameric
                     protein GrpK"
                     /protein_id="HFO6599591.1"
                     /translation="MKKRIINAPTPDILAMLKRRMPGEFRSRLDLIRIDAIGLLILPV
                     PDLYFYADVASKSANVVVSEIFGSCPQHITTLAIFGEVAAVHEAMRIIEEDDNQF"
     gene            10498..11073
                     /gene="grpL"
                     /locus_tag="ACHKUI_003453"
     CDS             10498..11073
                     /gene="grpL"
                     /locus_tag="ACHKUI_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system protease GrpL"
                     /protein_id="HFO6599592.1"
                     /translation="MKKILLITGDFSEDYEVMVPWQALNMLGFRVDVVCPGKRTGEFI
                     KTAIHDFEGDQTYTEKPGHLFRLTASFDDIRLQEYSGVYISGGRSSEYLRLNKSVLDI
                     VHYAMNLTLPVAAICHGPQILAAAGVLKGRKLTGYFTVKPEVEMAGGQWVTAADDEAI
                     VDGNLITATTWMGHPAILRHFITQMGTSIIH"
     gene            11122..13674
                     /gene="grpM"
                     /locus_tag="ACHKUI_003454"
     CDS             11122..13674
                     /gene="grpM"
                     /locus_tag="ACHKUI_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical diol dehydratase GrpM"
                     /protein_id="HFO6599593.1"
                     /translation="MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQ
                     LPAIMRRAKVVEKIFNQLPVTIRPDELIVGAVTINPRSTEICPEFSYDWVEKEFETME
                     HRIADPFVIPKKTAQELHEAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFG
                     GVGHVSVDYGKVLKIGFRGIINEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRF
                     AHRYAEEASRLAQQESNPTRKRELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQI
                     ESSGHSISPGRFDQYMYPYLESDKSISREFAQELVDCCWIKLNDINKTRDEVSAQAFA
                     GYAVFQNLCCGGQTEDGRDATNDLSYMCMEATAHVRLPQPSFSIRVWQGTPDEFLYRA
                     CELVRMGLGVPAMYNDEVIIPALQNRGISLRDARDYCIIGCVEPQAPHRTEGWHDAAF
                     FNVAKVLEITLNNGRVGNKQLGPVTGELTQFTSMEDFYTAFQKQMAHFVHQLVEACNS
                     VDIAHGERCPLPFLSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSVYAIQKQ
                     VFEDRKLSLSELKSALDANFGYPVGANPHTPAAKSSLNEQDIYDVVKRIIEQHGALDP
                     AAIKNEVYRQLTSGSAAPVQSGTMSRHEEIRRILENTPCFGNDIDDVDLVARKCALIY
                     CQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLAKEPLADGVSPRQGKDTL
                     GPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRNFSGLIRHYFDKKGMHVQF
                     NVIDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDDIISRTEQQLS"
     gene            13783..14700
                     /gene="grpN"
                     /locus_tag="ACHKUI_003455"
     CDS             13783..14700
                     /gene="grpN"
                     /locus_tag="ACHKUI_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical diol dehydratase-activating
                     enzyme GrpN"
                     /protein_id="HFO6599594.1"
                     /translation="MNKVEYETEGVIFNIQRYSLHDGPGIRTIPFFKGCPLSCKWCSN
                     PESQRHSPELLFKKNDCIRCGKCIDACPQQALSTTNAWFINRDRCIQCGKCTEICPTR
                     ALEMKGKRMSVTAVMRELEKEENLYRRSGGGITLSGGEPLAQPEFARELLKACKAKGW
                     HTAIETSGFTTKAVIDEVFPYVDLALTDIKAIDPEIHERNTGVNNRVILENLLRISFL
                     TKVVVRIPVIPGVNDNSQEIHNIAEFARLMQNVDTIHLLPYHSFGENKYNLLGRQYPM
                     GDTKSIEEAQMAELKAMVESLGFNCHIGG"
     gene            14837..15742
                     /gene="grpO"
                     /locus_tag="ACHKUI_003456"
     CDS             14837..15742
                     /gene="grpO"
                     /locus_tag="ACHKUI_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system permease GrpO"
                     /protein_id="HFO6599595.1"
                     /translation="MNSLDIVVAFGGGIFGAAVGALAAFEFVGLLVIAMTVVQIITGA
                     SSDFITFPFGLFGPHTGGFAAGVAATAYAAKKGKLGSGRDITAGLSGLAAYDVLLVGG
                     VFGAVGYIIAWGLNQIPAFPSGNAWTDTVALTVVISGVVSRLVFGKTGLFGKPEQGIR
                     HCYPPQDKCWIPYHSRIPQLSVLGLGIGLMAGFLGLKFGGNGALLAFGISAFSLIFLH
                     FNTQVPVSHHISLPAALVAVPSGSLIWAAIVGIICAILGELMSRIFLIHGDTHIDPPA
                     MVITIMTTMINLLATIGLFTLVPLF"
     gene            16228..16767
                     /locus_tag="ACHKUI_003457"
     CDS             16228..16767
                     /locus_tag="ACHKUI_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HFO6599596.1"
                     /translation="MMINEAQAQATATSGRGDGQYPSGLHDGAEISTAAGGQTKAEVP
                     LTMEAVITRENLMLAYQRVVENKGTAGVDNLSVAELKPWLKKNWRSVRQALIDGNYQP
                     RTIRRMDIPKPDGGVRTSGIPTVVDRLIQQAVQQAQRYIRGGKRWVVDMDLEKFFDRV
                     DHRLLMTRLARTIKDRRVL"
     gene            16834..17316
                     /locus_tag="ACHKUI_003458"
     CDS             16834..17316
                     /locus_tag="ACHKUI_003458"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO6599597.1"
                     /translation="MLQGGPLSPLLSNILLDELDKELERRGHSFCRYADDCNIYVSSR
                     KAGDHLLKNIRAFVENKLKVNEKKSAVARPWDRKFLGYSVTWHKQAKLKIALTSVNRL
                     KEKVHSLTTGNRSKSVKATINALTPVLCGWISYFRLTEVRGVLEELDGWIKRKLRCLL
                     "
     gene            17866..19128
                     /gene="grpP"
                     /locus_tag="ACHKUI_003459"
     CDS             17866..19128
                     /gene="grpP"
                     /locus_tag="ACHKUI_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization sensor histidine kinase
                     GrpP"
                     /protein_id="HFO6599598.1"
                     /translation="MIMFLKMQAESMKNKYKLNEFLDVKRLQTLQDNFSKSMMIALVV
                     VDENGIPVTKPSGFSDFCMRSRLNPTLAHHCYESDNAGGRAAMHARKPVVYRCHCGFV
                     EFAVPIMINDHYLGAFISGQVKVEEEKEQSISYILNNNDVWKDNPWLINLHQNTRRMP
                     YERFESTASTLLHVSSYLVEQAHTNNIQRELRKKDLALANELRKRVEIERSLHEAEFK
                     ALSYQINPHFLFNVLNTIGRLAFLEDAQRTETMVHDFSDMMRYLLRKNSHGLITLRNE
                     INYVNNYMSIQKVRMRDRFDYLCDIPEKYLDVVCPFLILQPLVENFFNYVVEPRDSNS
                     HLLIRATDDGLNVIIEVTDNGDGIAPDTINRILSGDQKLQKGSIGINNIKNRLKLLFG
                     ESYGLEIMSPNKPRMGTTIKLRFPMQEA"
     gene            19145..20200
                     /gene="grpQ"
                     /locus_tag="ACHKUI_003460"
     CDS             19145..20200
                     /gene="grpQ"
                     /locus_tag="ACHKUI_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization response regulator
                     transcription factor GrpQ"
                     /protein_id="HFO6599599.1"
                     /translation="MFTIVIVEDEPIELESLRQIISQCVENAAIHEASTGKKAIHLID
                     QLSQIDMILVDINIPLPNGKQVIEYLKKKNSDTKIIVITANDDFDIVRSMYNLKVDDY
                     LLKPVKKCILTDTIKKTLAFDEGENEKSRALKQKVFAMIDDCDYTQWHNFIFDTLNGA
                     CPVDMIEEDKRKDVIDFLEIVSQYMTAKGEKLMPTNRKVAALIKDINQYGMMESRYFR
                     IMISLLTISEELFDYALKGYTGNIDFIERAKFHIEKNILRNLTLDDVAAKSFVSACYL
                     SRTFKKITGFGFSNYIATRKITIAKSLLRFSDLQVNTIALELAYQDSNYFCRIFKKDT
                     GMTPSDYRKVVSPGITR"
     gene            20329..21480
                     /gene="grpR"
                     /locus_tag="ACHKUI_003461"
     CDS             20329..21480
                     /gene="grpR"
                     /locus_tag="ACHKUI_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system methionine
                     adenosyltransferase GrpR"
                     /protein_id="HFO6599600.1"
                     /translation="MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACEC
                     LVKTGATIVAGEISTHAAVDIEKIVRNTIKEIGYDHSRLGFDGNTCCVLNILGKQSAN
                     IADGIRGHSMEELGAGDQGITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPF
                     LLPDAKSQVTLRYQDNRVHSVDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLT
                     PQTRFLVNPAGSFVIGGPVGDCGLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLASRCEVQLGWAIGLPRPVSVRINTFGTQTVSSEALLRGVNSHF
                     DLSVFGIITTLDLLVPRYRKTACYGHFGRDSFPWEVTDKAALLYEDVGR"
     gene            21525..22070
                     /gene="grpS"
                     /locus_tag="ACHKUI_003462"
     CDS             21525..22070
                     /gene="grpS"
                     /locus_tag="ACHKUI_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization system shell hexameric
                     protein GrpS"
                     /protein_id="HFO6599601.1"
                     /translation="MSGKSLGLIETVGMSAAVEAADAAMKSANVSLVGYELTKGGGMV
                     TVKLEGEVGAINAAVAAAISAASRVGEVYAHKVIARTAQHIEQIIHSKVTAGVAEPQP
                     EVPAAQPTLQAENAPSQTFPVVNLDTSISASCMDVTDETLKSVEQHQPVKPSIENTKP
                     TKSDVPQEAAKKGPRSSGKKK"
     gene            complement(22427..22624)
                     /locus_tag="ACHKUI_003463"
     CDS             complement(22427..22624)
                     /locus_tag="ACHKUI_003463"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599602.1"
                     /translation="MVFLLVGILINAAEELRSTTESVTTITFKYQFDFQQTFTRAFTR
                     RFGIAPIAYRKLAKEEVRWNS"
     gene            complement(22563..22772)
                     /locus_tag="ACHKUI_003464"
     CDS             complement(22563..22772)
                     /locus_tag="ACHKUI_003464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6599603.1"
                     /translation="MNATIKNSSDINEFAKWIDENLEVELSINIEAKKAGYSKWYLQR
                     LFKHIIGISIGRYINKCSRRTTFYY"
     gene            complement(23043..23414)
                     /locus_tag="ACHKUI_003465"
     CDS             complement(23043..23414)
                     /locus_tag="ACHKUI_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFO6599604.1"
                     /translation="MSMEISTVVRHTKFAAEETRKQILDVAEFCFCETGFSKTTLEMI
                     AARAGCTRGAIYWYFNEKKDLLRQVIERVPLSLFDELTAIKNGRVSEPVKAMYRCLSR
                     SLIKLQYNVHLRNVIYIVFFL"
     gene            complement(23514..24659)
                     /locus_tag="ACHKUI_003466"
                     /pseudo
     CDS             complement(23514..24659)
                     /locus_tag="ACHKUI_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            24762..>24926
                     /locus_tag="ACHKUI_003467"
     CDS             24762..>24926
                     /locus_tag="ACHKUI_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6599605.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQE"
BASE COUNT         6517 a         5975 c         6369 g         6065 t
ORIGIN      
        1 gaaaagtgca gaaaattatt ccactgaatg cttttaatac agttggttag cttaaaaaat
       61 agcaattttt gatttcagtc attgaaaatt gcaatgcgtg acgattcaac agcatttaag
      121 tgagtttcag gatatgggaa ggacatgaaa agaggagcgc aataaaatac acaggaagaa
      181 ggggaaaatg tttcgtaaaa acagaaaatt aacagcgcac atcaggaaac gcagtctcct
      241 gattcagtgc taccacatga aaatttagtt aactgttacc gtactaaaat ttatgtcaga
      301 agatgttcca ttaccgaaag tcaatcagcg atacaaagac gatcatggag cactggccac
      361 cgtaaccagc gtcgaagaaa cccgagtggt ttttatgcgg gagggttatc cgcatccctg
      421 tatgcgaccg atgtacaact ttctgggaaa attcaaaccc gacccccggg aagaaacaga
      481 ataaaacgaa atccctgccg gcaatcacac tgcctgatgg cagcataatc cacttattaa
      541 ttctcatccc cggagacaag ccctgaaaac cactccgggt gttcattgta tgccgtgttc
      601 agcgcatccc aggaaacgtt gatataaaag ctgtcctgca atttgcgggt aaccacctct
      661 accacccggt tccggttata cgccagtccg gcataacctc tctctgccag ccactcattc
      721 agttcatgtt ccagagacag attgataata tcgtaatcgg accttgccat tcccgtttcc
      781 ttagcacatg ttttcaggca cggggataaa gtataaagaa tgtcgtactg ctgctgccag
      841 agcaatattc cccctgatgg ttgtatcaga gatggtatgc cggtattttg cgggttgttc
      901 ccgtcaggtt atcgtgcacc tccacggtcg ttgtcaccac cggcattccg ctctgtctta
      961 gtctcaggac atcagccgca atacgctgac tgccagacca gaacaggccg tccagtgcgg
     1021 tcaccagtaa ccccgcctcc agcgcatact tcagccgttc acggggcgct ttcacttccc
     1081 gggcaacctg ctgatatggc aatgatgtgt tttcattccc aaccacgcga cgaatacggt
     1141 gagacagatg ataccggtat gtatccggca cactggaaag actggccttc aggctgtaca
     1201 cgcagccaaa gcgtttatcg ttgaacacca catttttctg gctgatgtcc cattcttcac
     1261 gcagcacagc aatcagttgt ggtgtccggg taagcagaaa ggcaattcaa aacaggtgac
     1321 gtaatccaca ttcagcaggg caatacgcgg ccatttttct gatccattta caatcggatg
     1381 tgggcgaaca ttattatcat tatctgaaaa tctcccccta tggcatgttt gccatggcgg
     1441 agagtcattg aagcctttat gaggggtgaa tacattcctg aagcagtgca gtggcaaaaa
     1501 aacaccccgc ttttattcat tctctgacaa taaatattga gtttgactgt atcagatttt
     1561 aggtatttcc tagaaaagga cttttttgct caaggcaaaa caataccgca aaataatcct
     1621 tcacctgcac aaacatcctc agtccattaa aaaaatcgcc atgttaggtt aacgacatca
     1681 aatttggtcg tttaccatga ttaaggtagt tacacatggc ccgctattat ttttcgtgcc
     1741 ccaaaattta ttttggtgaa gaggcaataa cctctctatg ccaatttaat aataaacgag
     1801 tcggtattgt caccgatgat tttatgatgt catcaggtaa aactcgttat ttaataagcc
     1861 atatgcctga ggcatcggta tgcattttcg gtgaggttac tccggatcca gatattgata
     1921 ttctgacttc cggcgcggcg cgttttaaag cctttaagcc cgacgttatt atcgccctcg
     1981 gcggcggctc gtcgctggac gctgcgaaag gaattaaagt cacattggaa gagtattttg
     2041 gagatcatca aatcgatctc attgctattc ccacaaccag cggatccggc tctgaagtga
     2101 cgtcctacgc gatcatctct gatccgcaaa atggccgcaa atatccgttg atctccgggc
     2161 aactggtgcc ggaaatcgcc attctcgatc ctaacctagt gttaaccgcg ccgcgtcatg
     2221 tcgcggtaga taccggcatg gacgtgctga cccatgccat tgaagccttt gtctctactg
     2281 gctcaaatga ctttagcgat gcgctggcgg aaaaagccat cgcgttgacc tggcgctatc
     2341 tgccgcaggt gtttaacgac gaaaaagaca ttgcggcgcg cacccacatg cacaatgcct
     2401 cctgtatggc aggcatggca tttaactccg ccgggctggg gttggtacac ggcatggcac
     2461 acgccatcgg cgggatgctg catattcctc atggcaaaat taacgccatg ctgctgccga
     2521 ttgtcatcga cttcaacagc aaacgggcca gcactgaaac ccgcgatcgc tatcaccgtt
     2581 gcgcgcaaat cataggcatt gaccatgcgg taccggagca ggccatcgcg ctgatgactc
     2641 aggagatccg ccagatcaat cgccatttct gcatccccgt cactctcagc gaactgggca
     2701 tcgaccgtgc aaaaatcatc gagttgcgca gcgcactggt gaattcgacg ctggccgacg
     2761 gttgtaccgc gtcgaacccg cggcaggtga cgacgcatga tgtcgaaggg ttgatcgacc
     2821 tgattaccgg ctagctggat aactttgttc agcctccgtg aagcagtcaa aaaagtcctg
     2881 ctgataaaca aaaaccgggc gtcgttactc cctatcctga tcgttagacg cccattaccc
     2941 ataagataac ccaataatca ggtcgtaccc attatgaatt acaccgaaca acaagccgag
     3001 tggattaccc aacaaatttt gcaggaactt gccaccagta aaaaaacggc agcgcctgct
     3061 gccgcgcctg tcgcgctgaa tattccggtg ggcgtgtcca accgccatgt tcatctgtgc
     3121 cgtgaagata tggacattct gtttggctac ggctctacgc taacccgttc taaagcgttg
     3181 aaacagcccg gtcagtatgc cgccgaagag atggtgacgc tgcgcgggcc aaaaggcgag
     3241 ctcaacaaag tgcgcgtgtt agggccactg cgtaatgcca cgcaggtgga aatctctgtg
     3301 gccgacggct ttgcgctggg cactaaagct ccggtgcgca tgtcggggga tctgctggat
     3361 tcgccgggca ttgaaatcat cggtccgaaa gggcgggtgg tgaaacacaa cggcatgatt
     3421 gtcgcctggc gccatatcca tatctcgccc gaagaagccg aactgcacgg cctgcatgac
     3481 ggaatggaaa tcgatgtgca aacaaacggt ccgcgcggtg gtgtgctcag ccatgtagtg
     3541 gtacgcgtca cggcggatgc agtgctggaa ctgcatattg atatggaaga agctaacggc
     3601 gtcggtctgc gtaatggcga taccgttcgc ggcataaaac aggctgcgcg ccatggctaa
     3661 cagccccgaa aactgccgat ttcacgaatt ggccgcgctg attgacagcg gcgaatgtcg
     3721 gccctactcc ggcgcgttga aggtgggggg gatctcggta ccgccaatat cgcaatcgtg
     3781 gtgctggatg aagataacca gccggtggca ggggtaatgt atccctccaa tgtggtgaaa
     3841 gacggcgtgg tggtagatta cctgaccgcc agccgggtag tagcacgtct caaacaaatt
     3901 cttgaacaga aacagggctg cacgctggag cgcgcggcga tcgcagtccc accggggatc
     3961 tctgaaggca acacaaaagt gatcgtcaac gtagtggagt tggcgggttt tatcgtgacc
     4021 caagtactag atgaaccggt agccgctgcc agcgcgctga atattaaaaa cggggcggtg
     4081 gtggatgtcg gaggaggcac caccggcatg agcattttga aaagcggtaa agtgacgttc
     4141 agtgccgatg agcccaccgg tggaagccat atgaagttgg tgatcgctgg cgcgctgggc
     4201 ttgccttatg ccgaggcgga agaaatgaaa aaaaacgggc aggaagcgca ggtctttacc
     4261 ctggtgctac cggtggccca gaaaatggcc agcttggtgt cgggatggtt cgctacccag
     4321 caggtaaaaa atatttattt ggtaggtggc gcgagcagct tcagccagtt tgccaacgtg
     4381 ttcagtaaac aaaccggtaa aaatgtgatt ccttcggcag agccactatt ggtcacacca
     4441 ttaggcatcg ccatgaatgc ggaccagggg taaatcaatg gacaatcaaa ccttatcgca
     4501 actgctggac cgggtcatcg ctgaagtgtt ggcacaatcc gtcgcgcaga aaaatgtctg
     4561 cgtagtggtg actggtgagg atatctcctc gctgccggaa acgctgaact gccttgaggc
     4621 gcttgagctg gcgggttatc agttgtgggt aaatttttct cacagcgcca gccagtcggc
     4681 gctgaaatct gtctgcatgg atgcgctaat tcagcgtggt tctcgtgctg gatttgacag
     4741 atcgccgcct cactatgagg tgctctatct gcctgcgctc tctgtcaaca gcatgagcaa
     4801 aattgcgctg ggcattcgcg ataatctggc ctgtgaaact gtatttcagg cgttacagca
     4861 ccgtaaacag attatcgcca cgctgcatca acaatgcgtc gatagcgaat taccgatgcc
     4921 actgctgtct tgtctggaag gttacgctca ggtcctagag agctatggca ttacgctttt
     4981 cggtaaacgt gcaacccatg tcaagtcggt gcaaggaagc caatccaccc tgacagtagc
     5041 cggtaaaaaa cgtttaatca cccagagcga tatccgccta ctgaccattg gcacagtgct
     5101 gcgcattgag agcgacacgc tgattacccc tgctgccaga gatgaaatcc agcggcgtaa
     5161 tctcactgtt atccatggtt aaccggagga aatatgtacc tcgctaaagt tactggcgca
     5221 ttggtttcca ccaccaaaca cgcctcgctg aatggctcaa aactgctgat agtggcgcgg
     5281 ctcgatgaaa actatcagcc gactgtccat gcccaggtgg cagttgatac tgtcggcgcg
     5341 ggcaatggag agattgtgat catcaccacc ggcagttcgg cgcgtatgag caacaacaaa
     5401 gatcactcgg tgattgacgc tgccgtggtg ggtatcgtcg actcgctcga acttaacggt
     5461 taatagcggc gcgtacgctg aggaatactt atgtctgcga atgaggcttc actcgacaac
     5521 tggattaacg aggctattgc cacgcagtta ccggaactcc ggatgtcgct gagcctggag
     5581 gacagtgaac tgctggcaaa gttggtccgg gaaatagcac agtccagcaa tgtatcgatc
     5641 gtttttagcc tagtggatgc ctgcggtcag cagcgttttt tcttcagcat ggataacgcc
     5701 ctgctggtca gccatacctt ggcaccacaa aaggcctgga ccgcggtagc tctcaaaatg
     5761 ccaactcacg aactggcaac gctggtacag ccgggtggtg atttgtacgg ccttgagaaa
     5821 gaaaaagata tctgctgctt cggcggtggt tttccctgct ggtcgggcag gcgcttacta
     5881 ggcggcatcg gcatcagcgg cggcagtgtt gaggaagata tgctcatcgc ccgccaggcg
     5941 atggcgcagt ttagcgttct gcgttatccc ctcacaacaa cccgttaatt ttttgcagtc
     6001 catttcaggg ggcatcatgt ctttagcttg tgtattagtg attaattgtg gttcctcatc
     6061 aatgaaattc tccgtcattc ctcaggatgc agatcagccg ttgctgtcgg gcctagcgga
     6121 acgactgggt attgatcatg ctgtcataac gtttaaagac cgagacggcc ataaatccac
     6181 tgtcgcgctg gatgacgcca gccatcagca tgcgctcaaa gtgctcttcg cgaagctcga
     6241 cgaacagcaa ctgttggaag ccatcaacgc cgttgggcac cgcgtggctc atggcggcag
     6301 cgacttcaaa agatcagtac tggttaccga tgatgtgatc gaaaaagtcc gcgcgctgtc
     6361 ggtgctggcc cctttgcata accctgccaa cctgattggt attgaagcgg ctcgggctct
     6421 gttgcccgcg ttgccgcata ttgcggtgtt cgatactgcg ttccaccaga ccttgtctcc
     6481 ggctgcttac acctatgcga tcccgctgga gttccagcaa gattatatgg tgcgacgtta
     6541 cggctttcac ggaacatctc accgctatat tgccgccgag gcgcttgcat cactagatct
     6601 cgacccggcg gatcatggca tcgtgattgc ccacttgggc aacggttcat cgctatgtgc
     6661 ggtgcaaaac ggcacgagca ttgacacctc aatgggtatg accccgctgg aagggctggt
     6721 aatgggcacg cgctgcgggg atcttgattt tggtgtcgtc gcatatctgg cgaaacgcac
     6781 aggtcaaacg ttcgacacgc tgtacaaaat gctcaataac cagtccggcc taatggggat
     6841 ttccggctta tccagcgatt gccgcaccct tcagcaggcg cgtgatgagg gccacgaacg
     6901 ggcagcgtta gccatcgacg tgttagtaca tcgtctcgca cgacatatcg gcggccacgt
     6961 gacctcgctt aaacgcttcg atgcgctgat tttcaccggt ggaattggcg aaaactcggc
     7021 gttaattcga gaacttaccg tgcaacgcct tcaggcgctg ggattggaac tggataaaga
     7081 acgtaaccag cagctgttcg gcggacgtag cggactgatt tctaaaaccg acaccacgaa
     7141 gattgcggtc atcccaacca atgaagaaaa aatgattgct atagatgcag cggagattgc
     7201 ccgcacggca taactaattg gcaaaaatac gacagtttta acagtaatta tgattgctat
     7261 tcagagcggg gctattttgc aaggaagttt aaacgcagat cttttattac aaacctcggg
     7321 tggattttat tgtcaattgc gggaatgcaa aagcaagata gtcccactga cgcgcaaaac
     7381 attttgtctg aataacctta gcattatgat aattaaataa ttaatgcaca tgggtgaatc
     7441 cgtagattgc tattcctctg tgcacgtttt ctttctatcg gaaatattga ggtcgttatg
     7501 caacaagaag cattaggaat ggttgaaacg aaaggtctgg tttctgcaat tgaagctgca
     7561 gataccatgg tgaaatctgc caacgtgacc ctagtgggtt atgaaaaaat cggctccggc
     7621 ttggtgactg tgatggttcg cggcgacgtt ggcgcagtga aagccgcgac agatgcgggc
     7681 tccgctgctg cgagtaaagt gggagaactg gtctccgtac atgttattcc acgcccacac
     7741 attgaggtgg aaaaaattct accgaaggct atcggttaat aacaataaag gatactgatc
     7801 atgagagata atcttgttga gcagattata tctgacgtaa tgaataaaca aaccgatatc
     7861 tcaccggaag ctaaaacagc agcaaatttc gctggttgcg gtattacgga atttgtcggc
     7921 actgcgttgg gccacactat cggcctagtg attgccaatg ttgataatca attgcatgaa
     7981 gtcatgaata tcgacaagaa atatcgctct atcggtattc ttggcgcacg tactggcgca
     8041 ggcccgcaaa tttttgcggc tgacgaagcg attaaagcaa ccaacagcga aattatttct
     8101 attgagctgg cccgcgatac cgaaggaggt ggcggacacg gttgcttaat tatttttggt
     8161 gcgtctgatg tttccgacgt caggcgtgcg gttgaagtgg cgctgtctga aattgaacgc
     8221 accatgggtg acgtttatgg ctcgtcagcc ggacatcttg aattccagta caccgctcgt
     8281 gccagctacg cgctgaataa agcgctgggt gcgccgattg gtaaatcctt cgggatgacc
     8341 tgtgcctcgc ctgcggcgat tggcctagtg attgccgacg ctgcggcgaa atccgccgtg
     8401 attgacccgg tgggttatgc cagcccgtcg caaggcacca gttttagtaa cgaagtgatc
     8461 ttcaccttct ccggtgattc cggtgcagta cgtcaggcgg tgattgccgc gcgtgaagtc
     8521 ggtctacaac tgctctccac gttagatccg gtggaaatta aatccaccac gacgccatat
     8581 atctaagcat ctgatgacga tgcacgcggc gcggtcggta gatgaagagt agtaaggaat
     8641 ttaaagatga atgatattga aatcgctcag gctgtctcga ccatcctgag caaattcacc
     8701 aaagcaacac cggatgaagc tcccgccacg tcagaagcag cgcgggtcga cgggttggat
     8761 gaaattgtcg ctaaggcgtt ggcgcagcac tcttccgtgc gcgacgccag tgcgatctct
     8821 caggtagcga aggtagcgaa tgctagcact ggagcattcg ataccatgga tgaggcgatt
     8881 tccgccgccg tcttggcaca ggtacagtat cgccactgtt ctatgcaaga ccgcgcctcg
     8941 tttatcaatg gcattcgcga tgtcttcctg caggaagatg tgctgtgtgc cctttcccgt
     9001 atggcggtgg aagagaccgg catggggaat tacgaggaca aactgatcaa aaaccgcgtc
     9061 gcagcgctga aaacgccagg tatcgaagat ctgaccacca gcgcggtttc cggagacggg
     9121 ggtttaacgt tgatcgaata ctctgcgttc ggagtgattg gttcgattac accaacgact
     9181 aacccgaccg aaaccattat caacaacagc ataggcatgc tggcggcagg caacactgtg
     9241 gtgttcagtc cacatccgcg ctcgcgcaaa gtgtcgctgt acgcggttga gctgatcaac
     9301 aacaagcttg cccagttggg cgcaccggcg aacatggtgg taacggtgac caaaccgtcc
     9361 atcgacaata ccaacgtcct gatcaacgac ccacgtatca acatgctggt agcgactggc
     9421 ggtccagcca tcgtgaaaac cgtgatgtca tcaggtaaaa aagcgattgg tgcaggcgca
     9481 ggtaacccgc cagctgtggt agatgaaacg gcggatatcg aaaaagcagc ccgcgacatc
     9541 attaagggct gtagcttcga caacaacctg ccgtgcgtgg cggaaaaaga ggtgattgtt
     9601 gtgaaccagg tagcggacta tctgattcac tgcatgaaga aaagcggtgc ctatctgttg
     9661 tgcgataaga agttaagtca acaactgcaa tcgttggtgc ttaatgaaaa aggcactggg
     9721 ccgaataccg cctttgtcgg caaagatgcg cgctatatcc tgcaacaact gggaatccag
     9781 gttggcgacg atatcaaagt catcttaatt gaggctgaga aaacacatcc gtttgtggtg
     9841 catgagctga tgatgccggt cctgccggtg gtgcgcgtag acaatgttga tgaagccatt
     9901 gagctggcgg tgaaagtgga gcacggcaat cgtcataccg cggttatgca ctccaccaac
     9961 gtcgaaaagc tgaccaaaat ggcgcgcctg atccagacca ccatctttgt taaaaatggt
    10021 ccctcttatg cagggctggg ggtgggcggt gaagggcatg ccacctttac gattgccgga
    10081 ccgaccggcg agggacttac gtcagcgcgt tcgtttgcac gccgtcgtcg ctgtgtaatg
    10141 gttgaagcgc tgaatattcg ctaactatcg cgaagggtga cttaaagatg aaaaaacgaa
    10201 tcataaacgc accgaccccg gatattttgg ctatgttgaa acggcgtatg cccggggagt
    10261 ttcgttcgcg tttggatttg attcgcatcg acgccattgg tttgttaata ctgccggttc
    10321 cggatctcta tttttatgcc gacgtagcca gtaaaagcgc caatgtggtg gtatcagaaa
    10381 tctttggcag ttgccctcag catatcacca cattggccat ttttggcgaa gtcgcggcgg
    10441 tgcatgaagc catgcgaatc attgaggaag acgataacca gttttgagag tccgcttatg
    10501 aagaagatat tacttatcac aggcgatttc tctgaagact atgaagtcat ggtgccatgg
    10561 caggcattaa acatgttagg tttcagagtg gatgtagtgt gtccgggaaa acgtaccggc
    10621 gagtttatta aaaccgcgat tcatgatttt gaaggtgatc agacctacac cgaaaaaccc
    10681 gggcatcttt ttagattaac tgcatcattt gatgacataa ggttacagga atatagtggt
    10741 gtttatattt ctggtgggcg ttcatcggaa tacttgcgat taaataagtc agtattggat
    10801 atcgtgcatt acgcgatgaa tttaacatta cctgttgctg caatttgtca tggcccacaa
    10861 attctggccg ctgctggggt attaaaaggc cggaaactga cgggatattt tacggtaaaa
    10921 ccggaagtgg aaatggcggg tggccagtgg gtcactgccg cagatgatga agccattgtg
    10981 gatggtaatt taataaccgc gactacctgg atggggcatc cagcaatatt acgtcacttt
    11041 attactcaaa tgggcactag cattattcat taataccctg gcatctccgc aaggttaaca
    11101 aaatatattc gggagtttta tatgttagag aaaggctttt ctaatccgac agaccgtgtt
    11161 gtaagactga gaaatatgat cctgacggcg aagccgtatg tggaatcaga acgcgcggtg
    11221 ttagcaaccg aagcatataa agaaacagaa caactgcccg cgattatgcg tcgtgctaaa
    11281 gtggtggaaa aaatctttaa ccaattaccg gtaacaattc gcccagacga attaatcgtt
    11341 ggagcggtaa ctattaatcc tcgctcaact gaaatctgcc ctgagttctc ctacgactgg
    11401 gtggaaaaag agtttgagac tatggaacac cgtattgccg acccgtttgt tatcccgaaa
    11461 aagactgctc aggagcttca tgaagcattt aaatactggc cgggtaaaac taccagtgct
    11521 ctggcggcat cttacatgtc agaaggcact aaagagagta tggcgagcgg tgtattcact
    11581 gttggtaact acttcttcgg gggcgtcggt catgtcagtg ttgactacgg taaagtgctg
    11641 aaaattggtt tccgtggtat cattaatgaa gttagccgtg ccttggaatc attagatcgc
    11701 actgaaccag gatatataaa gaaagaacag ttctataatg cggtgctgat cagttataac
    11761 gcagcgatcc gtttcgccca tcgttacgct gaagaagcat cccgtctggc gcagcaggag
    11821 agcaatccga cccgtaaacg cgaactggag cagattgctc aaaactgtac tcgcgtgccg
    11881 gaatacggtg ctaccacatt ctgggaagca tgccagacat tctggtttat tcagagcatg
    11941 ttacagattg aatccagcgg tcattctatc tccccggggc gtttcgacca gtatatgtac
    12001 ccgtatctgg agtcggacaa atctatctcg cgtgaattcg cgcaggaact ggtggattgc
    12061 tgctggatca agcttaacga cattaacaaa acgcgtgacg aagtctctgc ccaggcgttt
    12121 gccggttacg cagtgttcca gaacctgtgc tgtggcggcc agacagaaga cggtcgcgac
    12181 gcaactaacg atctgagcta catgtgtatg gaagcgactg cccatgtgcg tctgccgcag
    12241 ccatccttct caatccgcgt atggcagggt actccggatg agtttctgta tcgcgcttgc
    12301 gagctggtgc gtatgggcct gggcgttccg gcaatgtaca acgatgaagt aatcatcccg
    12361 gcgctgcaga accgtggcat ttcgctgcgt gatgcgcgtg attactgtat catcggctgc
    12421 gtagagccgc aggcaccgca tcgcaccgag ggctggcatg acgccgcgtt ctttaacgtt
    12481 gcaaaagtgc tggaaatcac cctgaacaac ggtcgcgttg gcaataaaca gctaggccca
    12541 gtgactggcg aactgactca gttcaccagc atggaggact tctacaccgc gttccagaaa
    12601 cagatggcgc acttcgttca tcaactggtt gaagcctgta acagcgtgga tattgcccac
    12661 ggcgaacgct gcccgctgcc gttcctatca gcactggtcg acgactgtat cggccgtggt
    12721 aaatctctcc aggaaggcgg ggcgatttac aactttactg ggccgcaagc attcggcgta
    12781 gccgataccg gcgactccgt ctatgccatc cagaaacagg tattcgaaga tcgtaaactg
    12841 tctttgagcg aactgaaaag cgcactggac gccaacttcg gttatccggt gggtgcgaac
    12901 ccgcacactc cagcggcgaa atcctcgctc aacgagcagg atatctacga tgtggtgaaa
    12961 cgcatcattg aacagcatgg cgcgctggat ccggcagcga ttaaaaacga agtttatcgc
    13021 cagctgactt ccggcagtgc agcaccggtg caatccggca ctatgtcccg tcacgaggag
    13081 atccgtcgta ttctggaaaa cacaccgtgc ttcggtaacg acatcgatga cgtggattta
    13141 gtagcgcgta aatgtgcgct gatctactgc caggaagttg agaaatatac caacccgcgc
    13201 ggtggccagt tccaggctgg tatctatccg gtgtctgcga acgtgctgtt tggtaaagac
    13261 gttgccgcac tgccggatgg ccgtctggcg aaagagccgt tggcggatgg cgtatctcca
    13321 cgtcagggta aagacaccct tggcccaacc gccgcagcca actcagttgc caaactggat
    13381 cactttatcg cctccaacgg tacgctctat aatcagaaat tcctgccgtc ttctttggct
    13441 ggcgagaacg gtctgcgcaa cttcagcggc ctgatacgtc actacttcga taagaaaggg
    13501 atgcacgttc aatttaacgt tatcgaccgc aacactttga tcgaagccca gaaaaatcct
    13561 gagcaacatc aggatctggt ggtacgcgtt gcgggctata gcgcccagtg ggtagtttta
    13621 gcgaaagaag ttcaggacga cataatcagc cgtactgagc aacaactgtc gtaaatacca
    13681 gacgggctat gtatcaatat atagcccgtt actttctcca cgacttctgg ttgttacttt
    13741 tatctgaaaa ggtaattcat ctggtaaata tagagtgatg ttatgaataa agttgagtac
    13801 gaaaccgaag gcgtcatatt taatattcaa cgctattctt tgcatgatgg tccaggcatt
    13861 cgaaccattc ctttttttaa aggctgcccg ctgtcctgca aatggtgtag taatcctgaa
    13921 tcacaacgtc acagcccaga gttattattt aaaaagaatg attgcatccg ctgtggaaaa
    13981 tgtattgatg cttgcccaca acaggctctt tccactacta atgcctggtt tatcaatcgc
    14041 gaccgctgta ttcagtgtgg gaaatgtact gagatttgcc ctacccgtgc gctggaaatg
    14101 aagggcaagc gtatgtcggt aacggcagtg atgcgcgaac tggaaaaaga agagaattta
    14161 taccggcgtt caggcggcgg gattaccctg tccggtggtg aaccgctggc gcagccggaa
    14221 tttgcccgcg agctgcttaa agcctgtaaa gccaaaggct ggcacaccgc tattgaaaca
    14281 agcggcttca ccaccaaagc ggtgattgat gaggtatttc cctatgtcga tcttgctctg
    14341 accgatatta aagccatcga tccggaaatc catgagcgca acaccggcgt gaataacaga
    14401 gtgattctgg agaatctgct gcgcatctcc tttttaacca aagtggttgt gcgtattcct
    14461 gtgatcccag gagtgaatga caattcgcag gaaatccata atattgcgga gttcgcccgc
    14521 ctgatgcaga acgtcgatac cattcactta ttgccttatc acagcttcgg tgaaaataaa
    14581 tataatctgc ttggtcgcca gtatccgatg ggcgatacaa aaagtatcga agaagcccaa
    14641 atggcagagt taaaagcaat ggttgaatcc ttaggtttta actgccatat cggcggttaa
    14701 taaaagatgt aataagagtg cgaaaaaatg ctgcgcttgt ttatttgtta cgcatgaata
    14761 ctatttcaat tttaaaaata tagagcaatc tgcattactt atattgtcat ttgatttaat
    14821 tacagaggta agctagatga atagcttaga tattgtagtt gccttcggcg gcggcatatt
    14881 tggtgcggct gttggcgcgc tggcggcgtt tgaattcgtt gggcttctgg ttatcgccat
    14941 gacggtagtg caaattatta ccggagcatc atcggacttt attacttttc cgtttggact
    15001 gtttggcccg cacaccggcg gctttgccgc tggggttgcg gcgacggcgt atgcagcgaa
    15061 gaaaggcaag ctgggttccg ggcgtgatat cactgccggg ttgagcggtc tggcggctta
    15121 cgatgtgctg ctggtaggcg gggtttttgg ggcggttggt tacatcatcg cctgggggtt
    15181 gaaccagatt ccggccttcc cgtccggcaa tgcttggacc gataccgtgg cactaaccgt
    15241 ggtgatttct ggcgtggtct cgcgtctggt tttcggtaaa actggcctgt ttggaaaacc
    15301 ggagcaggga attcgtcact gctatccgcc gcaggataag tgctggatac cttaccatag
    15361 ccgcattccg cagctttccg tgctgggctt gggtattggc ttgatggcag gctttttagg
    15421 cctgaagttt ggcggcaacg gcgcgctgct ggcgtttggt atttccgcgt tctcactgat
    15481 tttcctgcat ttcaatactc aggtaccggt gtcacatcat atttcgctgc ctgccgcgtt
    15541 ggtagcggtg ccatcaggta gcctgatttg ggctgctatc gttggcataa tttgcgcgat
    15601 attgggtgaa ctgatgtcgc gtatcttcct gatccacggc gatacacata ttgacccgcc
    15661 ggcgatggtc atcaccatta tgaccacgat gattaatctg ttggcgacca ttggattatt
    15721 taccttggtt cccctgtttt aatcccgcca taacaaaaag ccagcagtgt ctgcctgctg
    15781 gctttttgtt atgtgcgccg tgactggcgc tgttcttcaa ctgtggtggc agaagaacga
    15841 ctaagaggtg aaagtcctcc acacacccgg tgaggggaag tgttagcgga aggcaagggt
    15901 gttcatcgcg aggtgaaatc tgaaggaagc tggatgcaaa atgctggcct gacgaacagg
    15961 aagcggttaa ggcggcgcag cagggtaagg tggcactaat caccaaagcc cgatactcac
    16021 acggaacgct gtgacgtaaa gccgacaggc ataagcagga aagtcgcgtg aattaccccg
    16081 ggagatctgt cgccctgcca gtgtgctacc ggcgccgtga ggcgccggga tggggcggca
    16141 gaagtcagcc gacgccgtag tagtgctgca aaaccggcag catgaagggc cgaacatgat
    16201 taactgtgat taggacatcc gaacccgatg atgattaatg aagcacaagc gcaggccact
    16261 gcgaccagcg gcagaggaga cggacagtat ccgtcagggc tgcatgatgg cgctgaaatc
    16321 agcacggcgg cgggtgggca aacgaaagcg gaagtgccgc tgacgatgga agcggtgata
    16381 acgagagaaa acctgatgct ggcttatcag cgcgtggtgg aaaacaaagg cacggcgggg
    16441 gtggataacc tcagcgtggc ggagttgaaa ccgtggctga agaagaactg gcgcagcgtc
    16501 aggcaggcat tgattgacgg aaactaccag ccgcggacaa tacgccggat ggatatcccg
    16561 aagccggatg gcggcgtgag aacctcaggc atcccgacgg tggtggacag gcttatccag
    16621 caggcggtgc aacaggcaca gcgttacata cggggtggca aacggtgggt ggtggatatg
    16681 gatctggaga agttcttcga ccgggtggat cacagactcc tgatgacgcg tctggccaga
    16741 acgataaagg acaggcgggt gctgtgactg atacgccgct acctgaaagc ggaaatggtt
    16801 aaagacgggc aaagggagaa acggcaggcg ggcatgctgc agggcggccc actgtcgccg
    16861 ttactgtcga acatcctgct ggatgaactg gataaagaac tggagcgtcg tggacacagc
    16921 ttctgccgct acgcagacga ctgcaacatc tacgtaagca gccggaaagc gggcgaccat
    16981 ctgctgaaga acatcagggc gttcgtggag aacaaactga aggtgaatga gaagaagagc
    17041 gcggtggcgc gcccgtggga tcggaagttc ctcgggtaca gtgtgacatg gcacaaacag
    17101 gcgaagctga agatagccct gacgagcgtg aacaggctga aggagaaagt ccacagtctg
    17161 acgacgggaa accggagcaa atcagtgaaa gcgacaatca atgctctgac accagtgctg
    17221 tgtggatgga taagctactt caggctgacg gaagtcagag gagtgctgga ggagctggat
    17281 ggctggatca aacggaaact gcgttgcttg ttgtagcggc agtggaagag accgctaacc
    17341 cgggcaaaga acctgcaaag agcaggactg agtaaggaca aggcaatgat ctctgcgtac
    17401 aacaaccacg ggccatggtg gaactcaggg tcaagccata tgaatcaggc gataaagaat
    17461 acatggttca ggaggcaggg tctgatatca ctgctggaac agtagaggca gttccagagt
    17521 taacatgaac cgccgtatgc ggaaccgcac gtacggtggt gtgagaggac ggcgggagtg
    17581 atcccgcctc ctactcgatc aattttaaaa cacctcctat gtaggtggtg atcgttcatg
    17641 tggggctttc tgacagactg cctcgtcatt ccattcgtat cgccagtaac tggcgtttgt
    17701 attgtggcag ttctggtata cgtttccctg tgtatctgca ctggttcagc aaggtttgaa
    17761 aaggagtatc ccttgcaaat aaccgaagca ttactttcag agccggagct gtttttgttg
    17821 ctgtgatcca tttcaatttt ttcgcgacaa attatttata aatatatgat tatgttttta
    17881 aaaatgcagg ctgaaagtat gaagaataaa tacaaattaa atgagttttt ggatgtaaaa
    17941 cgccttcaga ccctgcagga caacttttca aaatcaatga tgatcgcgct cgtggttgtg
    18001 gatgagaatg gtatcccggt cactaagccc agcggttttt cagacttctg catgcgttcg
    18061 cgtctcaacc cgacactggc gcaccactgt tatgaaagcg acaacgctgg tggccgtgcc
    18121 gcgatgcacg ctcgcaaacc ggtggtttat cgctgccact gcggttttgt cgaattcgct
    18181 gtcccaatca tgattaacga tcattatctc ggtgctttta tttccggtca ggtaaaagtc
    18241 gaagaagaga aagaacagag catttcctat attctgaata ataatgacgt ctggaaagat
    18301 aatccttggt taattaattt gcaccagaat acacgtcgca tgccgtatga gcgttttgag
    18361 tctactgcct cgacgttact acacgtatcg tcttatctgg tcgaacaagc gcacaccaat
    18421 aatatccaac gcgaacttcg taaaaaggat cttgcactgg ctaatgagtt aagaaaacgt
    18481 gtagagatag aacgctcact acatgaagct gaatttaaag cgctctctta tcagataaat
    18541 ccacactttt tatttaacgt attaaatacc attggccggt tagcttttct tgaagatgcg
    18601 cagcgtaccg aaacaatggt gcacgatttc tctgacatga tgcgctattt gttacgtaaa
    18661 aatagtcacg gcctgattac tctgcgtaat gaaataaatt acgtcaacaa ctatatgtcg
    18721 atccaaaaag tgcgtatgag agaccgtttc gattacctgt gcgatatccc ggaaaaatat
    18781 ctggatgtag tctgcccgtt tttgatcctg caaccgctgg tggaaaactt ctttaactac
    18841 gtagtggagc caagggatag caatagtcat ctgctgatcc gtgccacgga cgatggcctg
    18901 aacgtgatta ttgaagtcac tgacaatggc gacggcatcg caccagatac cattaatcgc
    18961 attttatcag gcgaccaaaa attgcaaaaa ggcagtattg gcattaacaa cataaaaaat
    19021 agattgaagt tgttatttgg cgagagctat ggcttggaaa taatgagccc taacaaaccc
    19081 aggatgggta caaccattaa acttcggttc ccaatgcagg aagcctgata caggaaaagg
    19141 acatatgttt accattgtta tcgttgaaga tgagcccatc gaattagaat ccctgcggca
    19201 gattatctcc cagtgtgtgg aaaacgctgc gatccatgaa gcctcaaccg gtaaaaaagc
    19261 gattcactta attgaccaac tgagtcaaat cgatatgatc ttagtggaca ttaatattcc
    19321 gttgccgaac ggtaaacagg tgattgaata tctgaagaaa aaaaacagcg atactaaaat
    19381 catcgtaatc acggccaatg atgactttga tatagtacgc agcatgtaca accttaaagt
    19441 ggatgattat ctgctcaaac cagtgaaaaa atgtatcttg accgacacca tcaaaaaaac
    19501 cctagcattc gacgaaggag aaaatgaaaa atcccgcgcc ctaaaacaga aagtgttcgc
    19561 catgatagac gattgcgatt acacccagtg gcacaacttt atttttgaca cgctcaacgg
    19621 tgcctgtccg gtggatatga tagaagagga taagcgcaaa gacgtgatcg attttctgga
    19681 aatagttagc cagtatatga ccgccaaagg ggaaaagtta atgccgacta accgtaaagt
    19741 cgccgcgctg atcaaagaca tcaaccagta tggcatgatg gaaagccgct atttccgcat
    19801 catgatcagc ctgctgacca ttagcgaaga gctgtttgat tacgcattaa aaggctatac
    19861 aggcaatatc gattttatcg aacgagcgaa attccatatt gagaaaaata ttctcagaaa
    19921 cttgacgctc gatgacgtgg cagcaaaatc gtttgtcagt gcctgctatt tgagccgtac
    19981 ctttaagaaa attaccgggt tcgggttttc gaactatatt gccacgcgta aaattactat
    20041 cgccaaatcg ttattgcggt tcagtgactt acaagtaaat accattgcgc tggaactggc
    20101 ataccaggat tcaaactatt tttgccgcat cttcaaaaaa gatactggca tgaccccctc
    20161 tgattatcga aaagtcgtgt ccccgggaat tacacggtga tggcgtgatt ttgcattggc
    20221 ccgataatga aagcaaaata gtccaacacg cccgcaagat agtttacgcc cacttgtcag
    20281 gttcctatcc taagatagga ataaacttcc caaacaagtg ggctcgcttt gaacgattac
    20341 ctgtttacct ccgaatccgt tgccgaaggg catcccgata aaatggcgga ccaaatttct
    20401 gatgccattt tggatgcgat actcctacag gacccgtggg gtaaggttgc ctgtgaatgc
    20461 ttggtgaaaa ctggtgccac tattgttgcc ggagaaatat ctacccatgc cgccgtggat
    20521 attgagaaga ttgtccgcaa caccataaaa gagattggtt acgaccattc gcgattgggg
    20581 tttgatggca atacctgctg tgtattaaat atccttggca aacagtccgc caatattgct
    20641 gacggcattc gcggccactc gatggaagaa ctgggggcgg gcgatcaggg catcaccttc
    20701 ggttacgcct gtgatgaaac gtcggaactg atgcccgcca cgctggtata cgcccatcgt
    20761 ctgatggagc gccaggcgca gttgcgtaaa tcccaacggt taccattttt actccctgat
    20821 gccaaaagcc aggttaccct gcgctatcag gataatcgtg ttcattcagt agataccgtt
    20881 gtggtgtcta cccaacacag ccctgacgtt tctctggacg cgctacggga agcggtcatc
    20941 gaagaaattg ttaaacctgt gatgccttcg cactggctca caccacaaac ccgctttctg
    21001 gtgaaccctg caggcagttt tgtgattggc gggccggtgg gtgactgcgg gctaacgggg
    21061 cgtaaaatta ttgttgatac ctatggcggc gcggcttgcc atggcggcgg cgcgttttca
    21121 ggcaaagatc cgtcgaaagt cgatcgttcc gccgcctacg ctgctcgcta tgtggcgaaa
    21181 aacatcgtcg ccgctgggct tgcctcacgc tgtgaagtcc agttgggatg ggccatcggc
    21241 ctgccccgcc ctgtttccgt caggattaac accttcggta cacaaacagt gtcatctgaa
    21301 gcgttgctga gaggcgttaa cagccatttc gatcttagcg tgttcggcat cattactacg
    21361 cttgatctgc tggtcccccg ctatcgtaaa acagcctgct atggtcactt tggtcgcgac
    21421 agctttccgt gggaagttac ggacaaagcg gcgctattat acgaagacgt tggacgttaa
    21481 gccgacgacg cgcagcacag ttgaatttat tacaggagaa atttatgtct gggaaaagtt
    21541 taggcttaat cgaaaccgtc gggatgtctg cggcggtaga agccgccgat gccgcaatga
    21601 aatccgctaa cgttagtctg gtaggctatg aattgacgaa aggcggcggg atggtgaccg
    21661 tgaagctgga aggggaagtg ggcgcaatta acgcggcggt agcggcagcc atcagcgcgg
    21721 caagccgggt tggcgaggtg tatgcccata aggtgatcgc ccgcaccgcg cagcatatcg
    21781 agcagattat tcactctaaa gtgaccgccg gcgtcgcaga acctcaacct gaagtgcctg
    21841 ctgcacaacc gacactgcag gctgaaaacg cgccttcaca gacattcccg gtggttaacc
    21901 ttgatacgtc aatcagcgca tcctgtatgg atgtcactga tgaaacgctg aagtccgtgg
    21961 agcagcatca accagttaaa ccctcaatag aaaacactaa acctacaaaa tctgatgtac
    22021 cgcaggaggc agcaaagaaa ggtcctcgtt ccagcggcaa gaaaaaataa gtaaattgct
    22081 atgacgctgc cgcggtaaca atttctttat cgcggcattt ttttgcttaa cagataaagt
    22141 aaaaaaccat ctgcatagcg ggcggctttg acacggcccc ctgaaagggg gcttaaacac
    22201 taccctttca ggcccagttg tatttgtctt tcttcttcct cttttgctac cttgttcgaa
    22261 aagggacgct cgtcctcgcc cactgttctg tatgcatacc tgctcccact gcaaattata
    22321 ttgctgggct atgttaacaa gcctatatgc tattaaaaac tcatgagcaa catattgata
    22381 atatcattct tatatatcca cgcttaagta aataacgctt acataactaa ctattccatc
    22441 tcacttcttc cttagcgagt tttcggtatg caataggagc tataccaaag cggcgagtaa
    22501 atgcccttgt aaatgtctgc tgaaaatcaa actgatattt aaaagtgata gtcgtgaccg
    22561 attcagtagt agaacgtagt tcttctgctg catttattaa tatacctacc aatagaaata
    22621 ccaatgatat gtttaaacaa tctctgtaaa taccattttg aatatccagc tttttttgcc
    22681 tcaatattta ttgagagttc tacctcaaga ttttcatcaa tccatttagc aaactcattg
    22741 atatcagagc tgttttttat agtagcgttc atttttatcg atgcctgtga aggttgattg
    22801 ggtcaactcc agtactatgc aaccctctct gaaaagggat ttatcatcag gttgcagaat
    22861 gcaacttcgc cccccccgta aacaaagaaa aaagcaggaa cgccagagtc tcactacgaa
    22921 tttccaaaca tatttctcta tataacttac ctttttcgaa aatataacat agtaatttca
    22981 caaaattatt gacatccacc attttttgta agaaatttat tcttatatca tccggacatt
    23041 cttcacaaaa aaaacacaat ataaattaca ttccgtaagt gtacattata ttgtaattta
    23101 atcaggctac gtgataagca acgatacatc gctttaaccg gttcagaaac tctcccattt
    23161 ttgatggctg taagctcatc aaataatgat agtggtaccc gctcgatgac ttgtcgcaga
    23221 agatcttttt tttcgttgaa ataccagtat atggcccccc gagtacaacc tgcgcgcgcc
    23281 gcaatcatct ccagtgtcgt cttcgaaaag cccgtctcac aaaagcagaa ctctgccaca
    23341 tccaggattt gtttgcgggt ttcttccgct gcaaattttg tgtgccgcac cactgttgaa
    23401 atctccattg acatcttatt tcaggaaata tctctggggc atttagtacc aacccaacag
    23461 ccatttactc aggcgcagta tgttgtttac tgcacctgaa tatttactca tgcttatgat
    23521 tgctcagctt cagtcttatc cgatgatttc ttatttctgc ggctgaagaa cccggcaatt
    23581 aacatataga agagaggaat ataaaagatt gccagtatcg tagcagtgat catccctcca
    23641 accacaccag taccgatggc atgtttactt ccggaacttg ctccagtcga gacagccagt
    23701 gggataaccc ccattacaaa tgccagcgaa gtcataatta tcggtcttaa cctcactctc
    23761 gccgcttcaa cagcagcctc tttaatgctt agtccttctt tctcatgcaa ttcacgagca
    23821 aactccacga taagtatggc atttttcgca gacagcccta ttgttgttaa taaaccgacc
    23881 tggaaaaaga catcgttacc aaggccgcgc aacaaagtag cacagacagc ccccaggaca
    23941 ccaagcggaa caaccagcat gaccgagaaa ggaatgctcc agctttcata cagtgcagcc
    24001 agacacagaa agacgatcaa cagtgaaagt gcatatagtg caggagcctg agaacctgaa
    24061 agtcgttcct caaaagacaa acctgtccag gaaatatgga aaccttcggg aagtcttgcc
    24121 gcaatatcct caattgcttt cattgcatcc ccggaactgt atcctggtgc cggttccccc
    24181 aggatttcga cagcagagat accattatat tgctccagtt ttggtgagcc ataaatccat
    24241 ttgcctgttg caaaagaggc aaaggaaacc atgtcgccat cactgttacg gacataccat
    24301 ttatcaaaat cctgcggtga tatacgtgag ccgggttcgc cctgaatata gacttttttc
    24361 acccgtccac gatcgttaaa atcatttaca tacatcgaac cccaggctgc tgacattatg
    24421 ttgtcgacgt cgctcatgct cagtttaaac gcctgaactt tctcgtcatc aatcagaatc
    24481 tggaactgag gctcatcgtt catgccgtta ggacgaacca tgtttaacgc tggacttttc
    24541 gccgcttcag caagaagctg atttcgggcc tggatcattt tttcatggct agctccgcta
    24601 cgatcctcga ggaacaggtc aaaccctgta gcattcccca gttctgatac ggcagggggc
    24661 gctttaaaac tagactagcc cccagaatct ccagacaacc aatatcactt atttaagtga
    24721 tagtcttaat actagttttt agaccagtca ttggagaaca gatgattgat gtcttaggac
    24781 cggagaaacg cagacggcgt actacacagg aaaagatcgc tattgttcag cagagctttg
    24841 aaccgggaat gacggtctcc cttgttgccc ggcaacacgg tgtggcagcc agccagttat
    24901 ttctctggcg caagcaatac caggag
//
LOCUS       DAZFRE010000078        24767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFRE010000078 DAZFRE010000000
VERSION     DAZFRE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.078"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            83..1165
                     /locus_tag="ACHKUI_003468"
     CDS             83..1165
                     /locus_tag="ACHKUI_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO6599606.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1667..2464)
                     /gene="ybcM"
                     /locus_tag="ACHKUI_003469"
     CDS             complement(1667..2464)
                     /gene="ybcM"
                     /locus_tag="ACHKUI_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6599607.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(2474..3025)
                     /gene="ybcL"
                     /locus_tag="ACHKUI_003470"
     CDS             complement(2474..3025)
                     /gene="ybcL"
                     /locus_tag="ACHKUI_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO6599608.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3194..3526)
                     /gene="emrE"
                     /locus_tag="ACHKUI_003471"
     CDS             complement(3194..3526)
                     /gene="emrE"
                     /locus_tag="ACHKUI_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6599609.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3871..4185)
                     /gene="fliE"
                     /locus_tag="ACHKUI_003472"
     CDS             complement(3871..4185)
                     /gene="fliE"
                     /locus_tag="ACHKUI_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6599610.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4400..6058
                     /gene="fliF"
                     /locus_tag="ACHKUI_003473"
     CDS             4400..6058
                     /gene="fliF"
                     /locus_tag="ACHKUI_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6599611.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            6051..7046
                     /gene="fliG"
                     /locus_tag="ACHKUI_003474"
     CDS             6051..7046
                     /gene="fliG"
                     /locus_tag="ACHKUI_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6599612.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7039..7725
                     /gene="fliH"
                     /locus_tag="ACHKUI_003475"
     CDS             7039..7725
                     /gene="fliH"
                     /locus_tag="ACHKUI_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6599613.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMAESEETIIEEVEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7725..9098
                     /gene="fliI"
                     /locus_tag="ACHKUI_003476"
     CDS             7725..9098
                     /gene="fliI"
                     /locus_tag="ACHKUI_003476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6599614.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9117..9560
                     /gene="fliJ"
                     /locus_tag="ACHKUI_003477"
     CDS             9117..9560
                     /gene="fliJ"
                     /locus_tag="ACHKUI_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6599615.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            9557..10684
                     /gene="fliK"
                     /locus_tag="ACHKUI_003478"
     CDS             9557..10684
                     /gene="fliK"
                     /locus_tag="ACHKUI_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6599616.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTAKGEPLVSDILSDAQQADLLIPVDETPPVINDEQSTLTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10789..11253
                     /gene="fliL"
                     /locus_tag="ACHKUI_003479"
     CDS             10789..11253
                     /gene="fliL"
                     /locus_tag="ACHKUI_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6599617.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11258..12262
                     /gene="fliM"
                     /locus_tag="ACHKUI_003480"
     CDS             11258..12262
                     /gene="fliM"
                     /locus_tag="ACHKUI_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6599618.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12259..12672
                     /gene="fliN"
                     /locus_tag="ACHKUI_003481"
     CDS             12259..12672
                     /gene="fliN"
                     /locus_tag="ACHKUI_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6599619.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSATDAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12675..13040
                     /gene="fliO"
                     /locus_tag="ACHKUI_003482"
     CDS             12675..13040
                     /gene="fliO"
                     /locus_tag="ACHKUI_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6599620.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13040..13777
                     /gene="fliP"
                     /locus_tag="ACHKUI_003483"
     CDS             13040..13777
                     /gene="fliP"
                     /locus_tag="ACHKUI_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6599621.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13787..14056
                     /gene="fliQ"
                     /locus_tag="ACHKUI_003484"
     CDS             13787..14056
                     /gene="fliQ"
                     /locus_tag="ACHKUI_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6599622.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14065..14850
                     /gene="fliR"
                     /locus_tag="ACHKUI_003485"
     CDS             14065..14850
                     /gene="fliR"
                     /locus_tag="ACHKUI_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6599623.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15140..15763
                     /gene="rcsA"
                     /locus_tag="ACHKUI_003486"
     CDS             15140..15763
                     /gene="rcsA"
                     /locus_tag="ACHKUI_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6599624.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15807..15995)
                     /gene="dsrB"
                     /locus_tag="ACHKUI_003487"
     CDS             complement(15807..15995)
                     /gene="dsrB"
                     /locus_tag="ACHKUI_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6599625.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16158..16385
                     /gene="yodD"
                     /locus_tag="ACHKUI_003488"
     CDS             16158..16385
                     /gene="yodD"
                     /locus_tag="ACHKUI_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6599626.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16683..17498
                     /gene="yedP"
                     /locus_tag="ACHKUI_003489"
     CDS             16683..17498
                     /gene="yedP"
                     /locus_tag="ACHKUI_003489"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6599627.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDEHMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(17495..19189)
                     /gene="dgcQ"
                     /locus_tag="ACHKUI_003490"
     CDS             complement(17495..19189)
                     /gene="dgcQ"
                     /locus_tag="ACHKUI_003490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6599628.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSQRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(19360..19542)
                     /gene="yodC"
                     /locus_tag="ACHKUI_003491"
     CDS             complement(19360..19542)
                     /gene="yodC"
                     /locus_tag="ACHKUI_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6599629.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19621..20538)
                     /gene="yedI"
                     /locus_tag="ACHKUI_003492"
     CDS             complement(19621..20538)
                     /gene="yedI"
                     /locus_tag="ACHKUI_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6599630.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20711..21631
                     /gene="yedA"
                     /locus_tag="ACHKUI_003493"
     CDS             20711..21631
                     /gene="yedA"
                     /locus_tag="ACHKUI_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6599631.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21620..22090)
                     /gene="vsr"
                     /locus_tag="ACHKUI_003494"
     CDS             complement(21620..22090)
                     /gene="vsr"
                     /locus_tag="ACHKUI_003494"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6599632.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIN
                     RLQALGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22071..23489)
                     /gene="dcm"
                     /locus_tag="ACHKUI_003495"
     CDS             complement(22071..23489)
                     /gene="dcm"
                     /locus_tag="ACHKUI_003495"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6599633.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23601..24251)
                     /gene="yedJ"
                     /locus_tag="ACHKUI_003496"
     CDS             complement(23601..24251)
                     /gene="yedJ"
                     /locus_tag="ACHKUI_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO6599634.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARLFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGEN"
     gene            complement(24291..24572)
                     /gene="drpB"
                     /locus_tag="ACHKUI_003497"
     CDS             complement(24291..24572)
                     /gene="drpB"
                     /locus_tag="ACHKUI_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO6599635.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6180 a         6383 c         6174 g         6030 t
ORIGIN      
        1 tgaaataatc tcattgatta gatgaatatt tgtagtgcag tgacatcatt tttaaataat
       61 agttcaaata aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc
      121 atcagtactg atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa
      181 gctggatctg tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga
      241 tggcgacaca acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact
      301 gactggtttc ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc
      361 ttccaaagat aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat
      421 cgattatggc cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc
      481 agaattcggt ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg
      541 tgttgcaacc tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc
      601 tcagtatcag ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg
      661 tttctccact acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga
      721 ccgcactaat gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc
      781 tgaagtatgg gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta
      841 ttctgaaacc cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa
      901 cttcgaagta gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct
      961 gcaatctaaa ggaaaagact tgggtgcgtg gggtgatcag gacctggttg aatatattga
     1021 tgtaggtgca acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa
     1081 cctgattgat aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc
     1141 tgtaggattg gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg
     1201 cctccccaac atataagtgg ccccctcaag ccactttcct tagaagcact atcttgcttc
     1261 tgactatata aaccttctgt tatatattac cctttatttt ctggggcgtt tcaacgcccc
     1321 atttttaata atactcagtg aacaattaac gtatcaatta gatttaataa caacgatatc
     1381 cgtacctaac cggatatcta atgtcataag catcccttta attatcccct caacctcttg
     1441 taactttccc actcttgaac tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt
     1501 gcatttctga aattgtgatg aaggaagata gtatcaatta tatgtgctta taaacgaccc
     1561 cgattagcgc agaagtcacc ggattgttca ggctccaata ggttgagtat gccgtattta
     1621 ttttccaaaa taaaaatcga tgctgttgct ccttcataaa atacttttac tctctggacg
     1681 cttttcttag ctctcttggt gttatcttaa aacgattatg gaacctttca gtaaaacgag
     1741 aaggacactt gtaaccattt tccctggcaa tctcgctgat gggttttact gtcgtttgta
     1801 tagcagacaa cgcattattt aacctcacgt cgtccaatat actttgaaaa cttattccct
     1861 cgcttgccag acggcgatgc aatgtggaaa cagaaatgta gagatatctc gctaccttgt
     1921 ttgctgtcca ttttgttcca ggttcggata gcattaggtt ataacaacga cttattaatg
     1981 attgtttact atctgataaa agtaaacaat taacatgatg tgctcctaat gaaagtagaa
     2041 cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc tcggaaaata gatgttttta
     2101 gttgctccca acaatatatt aactcggggt tctgaggtaa gcagaaacct gtcgtgttac
     2161 gaatttgatc agttatcgca taaaattttt gaaacttttc aattagatca atgggataat
     2221 aaagcatttc tgcaagataa agacctgctt ctggataatt cgcaatataa aattcatatc
     2281 cacagggaaa taatattatc tgattattat caacagttag actatgcgtc tcccaattga
     2341 taactttttt tccctgacgg atacgacata aagctggcat aagaggctta accctgtgaa
     2401 tctcatgatg tttatgcatc cgtatttctt caatctttag gttagactta tcttttgcca
     2461 gcatactctt accctacttt atctcataaa ctggtgttat ctcagcggtt gcaattttat
     2521 tggcattaag catataacca actaacgctc cgctggaatt agaatccaca ggaatcttat
     2581 cagtttttag agcccatact ttaaactggt aatgatgggg tttatctcct ttgggaggac
     2641 atgctccacc aaatcctgca taaccaaaat catttcggcc ttgaacagca ccagtcggaa
     2701 gttttgtacc atcacgtctt cctgcatcag tgggcaaata tgttacagtt gctggaatat
     2761 tagcaacagt ccagtgccac catccactgc ctgtaggagc atctggatca tacacagtta
     2821 cggcaaagct tttggtgcct tcaggagcac cagaccaggt taatgagggc gatgtattac
     2881 ccccttcaca cccaaatcca gaaaatacat gagacgttgt aagttgctct cctgttttta
     2941 tttcattact agtgacctga aatgctgcgg cctgcgcaga aaatgttatg aatgccaata
     3001 cacttgaaac gataagtttt ttcataaaaa cctcattgtt gtgacctatc gttattttat
     3061 ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga tcgcgcaaat gcaatcataa
     3121 ttataaaggt gccccgaaca ttagccataa tatgcacaaa tcagattcct gttatctcaa
     3181 ggataattat tttttaatgc ggtgcacttc gtgacaacaa gttaatgact aacacaccgg
     3241 cacaaatcaa catcatgcct atgatagctg gcaggtccag tcgttgacca aaaaatcccc
     3301 aggaaagcaa actaatcaga acgataccga ctcctgacca gatagcataa gcaatccctg
     3361 taggtatata agccagcgtc tgagctaata accagaatga tgcacaatag caaataattg
     3421 tgccaacaga gggccataac cgtgtaaacc cctctgaaaa cttcattaag gttgtaccaa
     3481 tgacctctgc aagtattgcg ccaccaagat aaatataagg gttcatagca tattctttcc
     3541 tgttcatact ggagagaatt gtactacagt ttgaacacaa ctcaccgttt tcatcctggt
     3601 gtatgtgtat ccagggggct ctggcgtggc aagagataaa ggcaggaaca ataatgatca
     3661 gaaggaaccc caggctaacg aatgagttca ctcttcctgg gagcaaagcg aagacaaaac
     3721 atgttatcgc gtttacggat cgtggatggc agaaaacaac caggatcagg tatccatttt
     3781 aaaccagaaa ttgagtgagt ttgccccatc catgccccac gctgtgggat cggatataat
     3841 taaacaagca tagtcaacag gttacacaac ctacacctgc atggacatca cttcctgata
     3901 cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg
     3961 catatcggtc atcacatcgt ttagcgccac gccgggttca ccgagggtga atttttctgc
     4021 ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg agcgcggcgt gcagctgccc
     4081 ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca tcgccgtagc
     4141 ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttcg tctcccggat
     4201 aatttctggt aacaaagcct accagtaagt caataagaca aaggcgctaa atagcaacaa
     4261 aaaaacgggt ttattggcgg atagaaaaaa acgaaagcac aaataatggg agcgtcaatt
     4321 tttcgagttt gctgacccgg gagtgagtct tgttccactt tgccaataac gccgtccata
     4381 atcagccacg aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc ttgagtggct
     4441 taatcgcctg cgtgcgaatc cgaaaattcc attgattgtt gccggttccg cagcagtggc
     4501 ggtcatggtc gcactgatcc tgtgggcgaa agcccccgac taccgcacat tattcagcaa
     4561 tctttccgat caggatggtg gcgcaattgt cagccaactg acgcaaatga atattcctta
     4621 ccgcttcagc gaagccagcg gcgctattga agttccggca gataaagttc acgaactgcg
     4681 cctgcgcctg gcgcaacaag gtttgccaaa aggcggcgcg gtcggtttcg aactgctcga
     4741 tcaggaaaag tttggtatca gccagttcag cgaacaggtg aattatcagc gggcgctgga
     4801 aggcgagctt tctcgtacca tcgaaactat cggcccggta aaaggggcgc gcgtacatct
     4861 ggcaatgccg aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg catcggtgac
     4921 ggtaaatctg ttacccggcc gcgcactcga tgaagggcaa attagcgcca ttgtacatct
     4981 ggtttccagc gcggtggctg gtctgccgcc gggaaacgtc acgctggtgg atcagggcgg
     5041 acatctgtta acccagtcca acaccagcgg gcgcgatctt aatgacgctc agttgaaata
     5101 tgccagcgat gtcgaaggcc gtattcagcg gcgtattgaa gcgatcctgt cgcctattgt
     5161 tggtaacggt aatattcacg cccaggtcac ggcgcaactg gacttcgcca gtaaagaaca
     5221 aacggaagaa cagtatcgcc ctaacggtga tgaatctcac gcggcgcttc gttcacgcca
     5281 gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc
     5341 gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccggcaa atcaaaataa
     5401 ccgccagcag caggcgagca ccaccagcaa tagtgggccg cgtagcacac agcggaatga
     5461 aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt
     5521 gcaacgtctg tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg gtaaaccatt
     5581 gcctctcagc aacgaacaga tgaagcaaat tgaagctctg acccgcgagg cgatgggctt
     5641 ttctgaaaaa cgcggtgatt cgctcaatgt tgttaactcg ccgttcaata gcagtgacga
     5701 aagcggcgga gcactgccgt tctggcaaca gcaagcgttt atcgatcagt tgctggctgc
     5761 cggtcgctgg ttgctggtac tgctggtggc gtggttgctg tggcggaaag cggtacgccc
     5821 gcagctaaca cgtcgcgccg aggcggtgaa gactgtgcag caacaggcac aggcccgcga
     5881 ggaagtggaa gatgcggtgg aagtccgtct gagcaaagac gaacaactcc aacaacggcg
     5941 cgctaaccaa cgactggggg cagaagtcat gagccagcgt atccgtgaaa tgtctgataa
     6001 cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc atgagtaacc
     6061 tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac cgggcggcag
     6121 aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca atggcgaacg
     6181 tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag caagaagctg
     6241 aacagtttgc cgcactgaat atcaacgcca acgattatct gcgttcggta ttggtcaaag
     6301 ctctgggtga agaacgtgcc gccagcctgc tggaagatat tctcgaaacc cgcgataccg
     6361 ccagcggtat tgaaacgctc aactttatgg agccgcagag cgccgccgat ctgattcgcg
     6421 atgagcatcc gcaaattatc gccaccattc tggtgcatct gaagcgcgcc caggccgccg
     6481 atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt atcgccacct
     6541 ttggcggcgt gcagccagcc gcgctggcgg aactgaccga agtactgaat ggcttgctcg
     6601 acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc gaaattatca
     6661 acctgatgaa aacgcagcag gaagaagccg ttattaccgc cgtgcgtgaa ttcgacggcg
     6721 agctggcgca gaaaatcatc gacgagatgt tcctgttcga gaatctggtg gatgtcgatg
     6781 atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgttg atcgcgctga
     6841 aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg
     6901 atattctgcg cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag gtcgaaaacg
     6961 aacagaaagc gattctgctg attgtgcgcc gtcttgccga aactggcgag atggtgattg
     7021 gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac gccagacgat
     7081 ctcgcgccac caccggcaga gtttgtgcca atggccgagt cggaagaaac cattattgaa
     7141 gaggtcgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc ccacgagcag
     7201 ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc acgaacaggg ctatcaggaa
     7261 ggactggccc aggggctgga gcaaggtctg gcagaggcga aggctcaaca agcgccaatt
     7321 catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc gcttgatagt
     7381 gtgatagctt cgcgcctgat gcagatggcg ctggaggcag cacgtcaggt cataggtcag
     7441 acgccaacgg tggataactc ggcactgatc aaacagatcc aacagttgtt gcagcaagaa
     7501 ccgttattca gcgggaaacc acagctgcgc gtacacccgg atgatctgca acgtgtggat
     7561 gatatgctcg gcgcaacttt aagtttgcat ggctggcgct tgcggggcga tcccaccctc
     7621 catcctggcg gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag tgtcgccacc
     7681 cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc acgcgcctga
     7741 ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg cctgcggtac
     7801 gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc ggattacaat
     7861 tgccgctcgg cgcaacctgt gtcattgagc gccagaacgg cagcgaaacg cacgaagtag
     7921 aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg gaggaagtgg
     7981 aaggtgtcct gcccggcgcg cgtgtatatg ccaaaaacat ttcggcagaa gggctgcaaa
     8041 gcggcaagca gttgccgctc ggtccggcgt tattaggtcg tgttctggac ggtagcggta
     8101 aaccgctcga tggcctgccc ttccccgata cgacggaaac cggtgcgctg attaccccgc
     8161 catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc gtgcgcccaa
     8221 tcaatgccct gcttaccgtc ggacgcgggc agcgtatggg gctgtttgcc gggtccggcg
     8281 ttgggaaaag tgtgctgctg gggatgatgg cccgttacac ccgcgccgat gtcattgtcg
     8341 tgggtttgat tggtgaacga gggcgcgaag taaaagattt tattgagaat atcctcggtg
     8401 ccgaaggacg tgcacgctcc gtggtgattg ccgctccggc ggatgtttct ccgctcctgc
     8461 gaatgcaggg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat cgtggtcagc
     8521 atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt gaaattgcgc
     8581 tggcgattgg cgaaccacct gccactaaag gttatccacc gtcggtgttt gccaaattac
     8641 cggcactggt cgagcgtgcc ggaaatggca ttagcggcgg cggctcgatt accgcgtttt
     8701 ataccgtgct caccgaaggc gatgaccagc aggaccccat tgctgactcc gcgcgggcaa
     8761 tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac tatccggcta
     8821 tcgatattga agcgtcgatc agtcgcgcaa tgacggcgtt gatcagtgag caacattacg
     8881 cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc gatctggtta
     8941 gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga caaagccatc gccctgtggc
     9001 cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg gaagcgtctc
     9061 tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa cggcagatgg
     9121 cggaacatgg tgcgctggcg accctgaaag atctggcaga aaaagaggta gaggatgccg
     9181 ctcgcctgct gggtgaaatg cgtcgcggat gtcagcaggc ggaagaacag ctcaaaatgc
     9241 tgatcgatta tcagaatgaa tatcgcaata acctcaatag cgatatgagt gccgggatga
     9301 ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa gccattactc
     9361 agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg aacagctggc
     9421 gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa tccacggcgg
     9481 cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc cagcgcgccg
     9541 ccatgaggaa acctgaatga ttcgcttagc gcccttgatt accgccgacg ttgacaccac
     9601 cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt tgttgagcga
     9661 agcattagca ggcgagacaa caaccgacaa agcggccccc cagttgctgg tggcaacaga
     9721 taagcccacg gcaaaaggcg agccgctggt cagcgatatt ctttcagacg cgcaacaagc
     9781 agatttactg atccctgtgg atgaaacacc gcctgtcatc aacgacgaac aatccacatt
     9841 aacaccgtta accaccgctc agacgatggc gttggctgcg gtggctgaca aaaatacgac
     9901 aaaagacgaa aaagcggatg accttaatga agacgtcacc gcaagtctga gcgccctttt
     9961 tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgcaccgt caaccgtgtt
    10021 accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca ctacagcaca
    10081 gcctgatgac gcccccggca caccagctca gccattaaca ccgctggtag cagaagccca
    10141 gagtaaagcg gaagtcatca gcacgccttc gccggtgacc gctgacgcca gcccgctaat
    10201 cactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga gtgcaccgct
    10261 gggttctcac gaatggcaac aatcattaag ccagcatatt tcgctgttca cccgccaggg
    10321 gcaacaaagt gcagagttgc gtctgcaccc acaggattta ggtgaagtgc aaatttccct
    10381 caaagtggat gataaccagg cgcagatcca gatgatttca ccgcatcagc acgtacgcgc
    10441 cgccctggaa gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg gcattcagtt
    10501 agggcaaagc aacatcagtg gcgaaagctt tagtggtcag cagcaggccg cttcccagca
    10561 acagcaaagc caacgcacag caaaccatga acctctggcg ggggaagacg acgatacgct
    10621 tccggttccc gtctctttac aagggcgtgt aacaggtaac agcggcgttg atattttcgc
    10681 ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc gctcaagacg
    10741 caggataatt agccgataag cagtagcgac acaggaagac cgcaacacat gactgattac
    10801 gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt cattaccctc
    10861 gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc cgctgacgac
    10921 aaagcgcagc aacgcgtcgt gccctccccg gtcttctatg ctctggatac ctttacggtc
    10981 aatttgggcg atgcggatcg cgtgctttat atcggcataa ccctgcgact gaaagatgaa
    11041 gcaacccgct cgcggctgag tgagtatttg ccggaagtcc gtagtcgctt gctgttactg
    11101 ttttcgcgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa cctgattgcc
    11161 gagattaaaa ccacactttc caccccgctt gttgccgggc aaccgaaaca ggatgtcacc
    11221 gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta ttctttctca
    11281 agctgaaatt gatgcgctgt tgaatggcga cagcgaagtc aaagacgaac cgacagccag
    11341 tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac gggttgtgcg
    11401 cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt ttcgtatggg
    11461 gctgttcaac ctgctgcgcc gtagcccgga cattaccgtc ggggccatcc gcattcagcc
    11521 gtaccatgaa tttgcccgca acctgccggt gccgaccaat ctgaacctta tccacctgaa
    11581 accgctgcgc ggcacagggc tggtggtgtt ctcgccgagc ctggtgttta tcgccgtgga
    11641 taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg agtttaccca
    11701 taccgaacag cgcgtcatca accgcatgtt gaaactggcg cttgaaggct atagcgacgc
    11761 atggaaggcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc aggtgaaatt
    11821 taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc atgtggagat
    11881 tggcaacctg accggcgaat ttaatatctg cctgccattc agcatgatcg agccgctacg
    11941 ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga actggcgcga
    12001 taacctggtg cgccaggtgc agcattcaca gctggaactg gtggccaact ttgccgatat
    12061 ctcgctacgc ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc cgatagaaaa
    12121 acccgatcgc atcatcgccc atgttgacgg cgtcccggtt ctgaccagtc agtacggcac
    12181 tctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt taaattctct
    12241 gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa caacggcgca
    12301 atggacgatc tgtgggctga agcgttgagc gagcaaaaat caaccagcgg aaaaagcgct
    12361 accgatgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt gcaggatatc
    12421 gacctgatta tggatattcc ggtcaagctg accgtcgagc tgggccgtac gcggatgacc
    12481 atcaaagagc tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg tctggcgggc
    12541 gaaccgctgg atattctgat caacggttat ttaatcgccc agggcgaagt ggtggttgtt
    12601 gccgataaat atggcgtgcg gatcaccgat atcattaccc cgtccgagcg gatgcgccgc
    12661 ctgagccgtt agtgatgaat aaccatgcta ctgtgcaatc ttccgcgccg gtttctgctg
    12721 agccactgct gcaggtgagc ggcgcactca tcgccattat tgccctgatc ctcgctgctg
    12781 cctggctggt aaaacggctg ggatttgccc ctaaacgcac tggcgttaac ggtctgaaaa
    12841 ttagcgccag tgcttcgctg ggcgcgcgtg aacgggtcgt ggtggtcgat gtggaagatg
    12901 cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa cttccccctt
    12961 cagcaccaac ggaagagata ccgcagaccg attttcagtc ggtcatgaaa aatttgctta
    13021 agcgtggcgg gagatcctga tgcgtcgttt gttttctgtc gcaccggtcc ttctctggct
    13081 gattacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc tacctggcgg
    13141 tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt tgacgttcat
    13201 tccagcgatt ttactgatga tgaccagttt cacccgcatc attattgttt ttggtttatt
    13261 gcgtaacgcg ctgggaacgc cctccgcgcc acctaaccag gtattgctgg ggctggcact
    13321 gtttttgacc ttttttatta tgtcgccagt gatcgacaaa atttatgtcg atgcgtacca
    13381 gccattcagc gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg cgcagccgct
    13441 gcgtgagttt atgctgcgtc agacccgcga ggcagattta gggttgtttg ccagactggc
    13501 gaataccggc ccgttgcagg ggcctgaagc tgtgccgatg cgcattttgc tcccggccta
    13561 cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca tccctttttt
    13621 gattatcgac ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggttccccc
    13681 cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg gctggcaatt
    13741 gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga cacctgaatc
    13801 ggtcatgatg atgggaactg aagcgatgaa agtcgcgctg gcactggcag ccccgctgtt
    13861 gctggtggcg ctggtcacgg gccttatcat cagtattttg caggcagcca cgcagattaa
    13921 cgaaatgacg ctgtcgttta ttccgaaaat catcgccgta tttatcgcca ttattattgc
    13981 cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca ctaacctgcc
    14041 gtatatcatc gggtagcccg tacaatgatg caggtgacaa gcgatcaatg gctttcctgg
    14101 ttaagcctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac cgcgccaatt
    14161 ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat gatcacattc
    14221 gccattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg
    14281 ctggccgtgc agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt
    14341 gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc atttgcgaca
    14401 tttgtcgatc ctggcagcca tctcaatatg cccgttttag cgcgcatcat ggatatgctg
    14461 gcattactgc tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat
    14521 acctttcaca ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt tctggcactc
    14581 accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact
    14641 ctgttgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc acaattatcc
    14701 atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta
    14761 atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa tttgctggct
    14821 gatattatta gtgaattacc attaatataa ttccgtaacg tttatcatgc taccctaagg
    14881 attatccgaa aaataatatc tgcgaacatc ttccaggata ctcctgtagc gaaatatttg
    14941 ttttaagctc actcacatat cgcaacattt actttacttt aagacaattc caggcaaatt
    15001 atacaacact ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt
    15061 gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg
    15121 cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact
    15181 aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga
    15241 aaccgttgat gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa
    15301 tgaggactgt ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca
    15361 acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata
    15421 tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga
    15481 cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc
    15541 gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg
    15601 aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg
    15661 taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact
    15721 gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg
    15781 tttcccacca gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga
    15841 tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga aaccaggtac
    15901 attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg cggaccgcca
    15961 tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc
    16021 agtgtagaac aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct gattttctcg
    16081 acggtctata cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac
    16141 ggttgcgagg aaagatgatg aaaaccgcaa aagagtacag cgataccgca aaacgtgagg
    16201 tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc
    16261 atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc
    16321 gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc
    16381 gttgaatgca caataaaaaa atcccgaccc tgagggggtc gggatgaaac ttgcttaagc
    16441 aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc
    16501 aatttttttc gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata
    16561 gaaatgtgac gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa
    16621 atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga
    16681 ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg
    16741 acagtcatag ttatgactgg caaccggcag ccccttggct cagccgttta cacgaagcca
    16801 atattcccgt cattctctgt agcagtaaaa cttcagcgga aatgctgtac ttgcaaaaaa
    16861 tgttggggct acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg
    16921 aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa
    16981 tctgccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg
    17041 atgtcgacga tgcaaccatt gccgaatgga cgggattaag ccgtagccag gcggcgctga
    17101 cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag catatggcac
    17161 aatttatcgc tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc
    17221 acgtactgga tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc
    17281 aacaattgtc aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc
    17341 ctttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtcc
    17401 atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct
    17461 ggcgtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc
    17521 gattgcgccc ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt
    17581 caaaatcata atcaccggtt tcctcgctgc tgcttacccc cagcgaggca ctaatgcgta
    17641 tcgttgtact cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg
    17701 cgacctccgc agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac
    17761 cgacccgccc ggcaatgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag
    17821 aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa
    17881 gatcgacctg aatgacagaa aaaggatgtt ggtgcgtgtg acacaattta gcgagcggac
    17941 aggctttttc gaacaatgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct
    18001 gccactgcaa cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc
    18061 aggagaggag taacatgctg gtaaagagcg cccacagcag ggttaatgca atgctgatac
    18121 tgccgaaatc gccgcgcacg ccttcgctta gcgtatggac gcgcaccagc acaccgtcga
    18181 aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt
    18241 catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg
    18301 gatggtcagg atttgaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac
    18361 catcgaggtt tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac
    18421 tcatccccag cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct
    18481 gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac
    18541 gattttctcg ttgtgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg
    18601 ttggtacatt gcgcgtaaac aaggtcggca gcgtcgaaac gtaaaatcca gcacgcgaga
    18661 catacacagc ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa
    18721 cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggagattcc
    18781 cctcgctgac taatgcatcc gatacgccat tgactggcag ggtgcgccgt cggttgagtt
    18841 cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgctgag
    18901 aggtgaaatc cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt
    18961 tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac
    19021 gctggctgga tatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg
    19081 aaaaaagcaa taccacgata aagcagagat taacgatatg accaggcccc aggcggcgtg
    19141 cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc
    19201 ccaacctgct tttttatgat tctggcataa cccggcgata gcgtaccgca aaacagcgat
    19261 aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca ttgggacacc
    19321 aggccggatg tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg
    19381 actcccctcc cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc
    19441 gtcataccaa cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc
    19501 gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg
    19561 agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta
    19621 ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact cccagcacca cgatgccgcc
    19681 gatgataaat ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact
    19741 ttgctgcccg gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat
    19801 cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca
    19861 gggcgcgata atcaataacc ctttacctaa tgcctgcatc agcgcgctgg atttttccgc
    19921 cagccaatac cccaggtcat caatcttaac gataacccct accagaccgt agacgcccac
    19981 ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc
    20041 cacaatcccc agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc
    20101 ccctttaatt ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg
    20161 ctgctgactc tgcgccggat cttctttatg tttacgcgcc tccagcatat gcagcacttt
    20221 ctctacccct tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca
    20281 cgggataaac gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga
    20341 tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg
    20401 ttgcgcattg agcgatagat catcccctaa tacaccggcg gttttcttcg ccgccagttt
    20461 gcccatcacg gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact
    20521 actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa
    20581 aaacggcagc tctgatactt gcttcacagg tcaacatttt tttaacaatc aagtgcaatt
    20641 atatctctcg ccagcagaat gattttcacg tttactatga accacctttt tatttccacc
    20701 gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta
    20761 tatcatttgg ggctcaacct attttgtcat tcggattggc gtggaaagct ggcctccgtt
    20821 aatgatggcg ggcgttcgat tcctggcagc cggtatttta ttgctggcat ttttgctact
    20881 gcgcggacac aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt
    20941 attgctggct gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg
    21001 catcgccgcc gttgtggttg caaccgtgcc cctctttacc ctgtgcttca gccgcctgtt
    21061 tggcattaaa acgcgcaaac tggaatgggt gggtatcgcc attgggcttg ccggaatcat
    21121 catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat
    21181 cggctcgatt agctgggcgt ttggctcggt ttatggctcg cgcattacct tacctgtagg
    21241 gatgatggcg ggtgcgattg agatgctggc ggctggcgtg gtgttaatga tcgcgtcgat
    21301 gattgcgggt gaaaaactga cggcgctccc ttccctttca ggcttccttg cggtcggcta
    21361 tctggcgctg tttggttcga ttatcgccat taacgcttat atgtatttaa tccgcaacgt
    21421 cagtccggct ctcgccacca gctacgccta cgttaaccca gtggtcgcgg tcttgctggg
    21481 tacgggactg ggcggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt
    21541 tttcgcggtg gtactggtca cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc
    21601 gccagttatt caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc
    21661 tggcaccttc gccgcagatc cactcttcca gacgctcggt aagcgcttca tccgtcagct
    21721 tctcgcgccc acgtaacgca cactcccaga caatcagtac gcgccagccg agtgcctgca
    21781 agcgattgat atcgcggcga tcgcgctcaa catttttacc tatcttctcc agccagaact
    21841 cggttcgggt cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt
    21901 gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg
    21961 cgtcctgaac gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa
    22021 tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa
    22081 cgtcgaccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca
    22141 agcagttttg ccaccgcggc aaagaccggc acgaccaccg agttaccgaa ctggcgatag
    22201 gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt
    22261 aagcgcgcgc attcacgagg tgttaaccga cgtggacgat gttgctgatt cagcggatcg
    22321 tcaaagtctt tctcacccgt ggccatatcc cagccgcggt cgattaaaat ttccgcgcca
    22381 tctttgtaat aacgagcaga cagcgtacgg gtgacacttt gcggattgtt cggataaacc
    22441 attccataac cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagaggtac
    22501 ttccacagca ccggcgtcag gatatatttt gcctcaacca tcgggtccag cagctgcgcc
    22561 agcgtcactc gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc
    22621 agattcagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa
    22681 tgtttaccgt cgatgatttt cggatcgtcc ggcccgttat cttctgcgtc ggcaacgtca
    22741 tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactt
    22801 ttcaggtttt tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc
    22861 accacatcaa agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc
    22921 gagttctttt tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa
    22981 acatcgtgtt caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct
    23041 tcttgatgac tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg
    23101 caataatggt tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg
    23161 cactgtccac caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg
    23221 ataaagcgaa acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga
    23281 tgggcaaact ctttctcact taaacggtgc catgcagagt cattcgccag cgcacgtttt
    23341 aaaattgccg cgctccagtg attctcaccc acaccattaa gctgcgccac cagcgtttta
    23401 acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat
    23461 gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca
    23521 ggttaacata attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct
    23581 atcaccttgt gatcgatacc ttaattctcc cccgccagtt cggcaccgag cttcgccata
    23641 aactcgacta aaaattgcgc gttgtgctgt gccagctgct tgcctcttgc ggtttgcatg
    23701 gtttgcggta atttcagtaa ctttgtctgg aaatgatcca gcgcgtagcg tttatcatca
    23761 agcgggcgat gctgtgcgaa cggatcttcg ccatcaaaca gcgccaagcc caatgcccct
    23821 gaaacggcaa ataagcgcgc caggccaatc gcccccaggg cttccagccg atccgcatcc
    23881 tggacgattt tagcttccgt tgttaagggg gcgatttgcg cgctgaaact gtgagcggca
    23941 atggcatgac aaacggcctc aattttctcc gccggaaatt gcacgaactc ttcacggagc
    24001 aggcgacgcg tctcttctgc cgccaggatt gaagaacgct gccgttgcgg atgatttttc
    24061 gccaggctca cgatatcgtg aaaataacag gcggttaaaa tcaccagcat atcgacatcg
    24121 tcatctgccg ccagtttctg tgccgtagcc cagacacggc gaaaatgaca caggtcatgc
    24181 gccgcgtcct gatgttgatg atggtttttc aaccagttct cgaactgtgc ctgccagtgt
    24241 tgtaactcca tacgcgctcc gttgccaaag agtcgcaacc ttagcagttt ttattcatag
    24301 cgtctgcaac gtgcgggacg aggtcgggtg taccatgcaa tgccgcctaa caaggcacca
    24361 accagggcca taaacagaaa tccgaccaat gctcctaacc acttccccgc ggtagatcct
    24421 aaatcacttt caaatcccga cacgggggca tcggggatcc ggctcatcac ccagatgtat
    24481 cgcgccattg cccagacgaa atagccacac catgtataga aagcccacaa agccaatttg
    24541 ccgccagggc tgcgagagag tctctcttcc atctttgtcg aaccgtattc cattttgaga
    24601 taacccggaa aattcagact tagaactatt atgtgataaa aatcacattt ttccacattg
    24661 gatgagccag tcaagactca atcatttttt gaacaaatag ataaattttg cgcaacggcg
    24721 gtgaatacag gaaaattcga tttgaacgat tttatttatc tttgatt
//
LOCUS       DAZFRE010000079        22900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFRE010000079 DAZFRE010000000
VERSION     DAZFRE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.079"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(304..456)
                     /gene="hokE"
                     /locus_tag="ACHKUI_003498"
     CDS             complement(304..456)
                     /gene="hokE"
                     /locus_tag="ACHKUI_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6599636.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            425..678
                     /locus_tag="ACHKUI_003499"
                     /pseudo
     CDS             425..678
                     /locus_tag="ACHKUI_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            902..2020
                     /locus_tag="ACHKUI_003500"
     CDS             902..2020
                     /locus_tag="ACHKUI_003500"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6599637.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HHQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2086..2334
                     /locus_tag="ACHKUI_003501"
     CDS             2086..2334
                     /locus_tag="ACHKUI_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6599638.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2399..2782
                     /locus_tag="ACHKUI_003502"
     CDS             2399..2782
                     /locus_tag="ACHKUI_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6599639.1"
                     /translation="MDKQSLHETAKRVALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            2861..3514
                     /gene="nfsB"
                     /locus_tag="ACHKUI_003503"
     CDS             2861..3514
                     /gene="nfsB"
                     /locus_tag="ACHKUI_003503"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6599640.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3622..4869
                     /gene="ybdG"
                     /locus_tag="ACHKUI_003504"
     CDS             3622..4869
                     /gene="ybdG"
                     /locus_tag="ACHKUI_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6599641.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4950..6326)
                     /gene="pheP"
                     /locus_tag="ACHKUI_003505"
     CDS             complement(4950..6326)
                     /gene="pheP"
                     /locus_tag="ACHKUI_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6599642.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6428..9571)
                     /gene="cusA"
                     /locus_tag="ACHKUI_003506"
     CDS             complement(6428..9571)
                     /gene="cusA"
                     /locus_tag="ACHKUI_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6599643.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLLILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9583..10806)
                     /gene="cusB"
                     /locus_tag="ACHKUI_003507"
     CDS             complement(9583..10806)
                     /gene="cusB"
                     /locus_tag="ACHKUI_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6599644.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10822..11154)
                     /gene="cusF"
                     /locus_tag="ACHKUI_003508"
     CDS             complement(10822..11154)
                     /gene="cusF"
                     /locus_tag="ACHKUI_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6599645.1"
                     /translation="MKKTLQVAMFSLFTVIGFNAQANEHHHETMSEAQPKVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGSLSL
                     LQDIKVSQ"
     gene            complement(11178..12560)
                     /gene="cusC"
                     /locus_tag="ACHKUI_003509"
     CDS             complement(11178..12560)
                     /gene="cusC"
                     /locus_tag="ACHKUI_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6599646.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYFATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPVFNAGRNQANLDIAEIRQQQSVVNYEQKVQNAFKEVADALALRQTLNAQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12717..13400
                     /gene="cusR"
                     /locus_tag="ACHKUI_003510"
     CDS             12717..13400
                     /gene="cusR"
                     /locus_tag="ACHKUI_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6599647.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13390..14832
                     /gene="cusS"
                     /locus_tag="ACHKUI_003511"
     CDS             13390..14832
                     /gene="cusS"
                     /locus_tag="ACHKUI_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6599648.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRNTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTRFVIILPA"
     gene            14982..17219
                     /gene="nrfB"
                     /locus_tag="ACHKUI_003512"
     CDS             14982..17219
                     /gene="nrfB"
                     /locus_tag="ACHKUI_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6599649.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVICARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVRKYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            17206..20178
                     /gene="nfrA"
                     /locus_tag="ACHKUI_003513"
     CDS             17206..20178
                     /gene="nfrA"
                     /locus_tag="ACHKUI_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6599650.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGVTLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQDDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPSIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINITPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            20179..21069
                     /locus_tag="ACHKUI_003514"
     CDS             20179..21069
                     /locus_tag="ACHKUI_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6599651.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSLDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            21717..22670
                     /gene="ompT"
                     /locus_tag="ACHKUI_003515"
     CDS             21717..22670
                     /gene="ompT"
                     /locus_tag="ACHKUI_003515"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO6599652.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DRDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT         5677 a         6069 c         5657 g         5497 t
ORIGIN      
        1 ggtttgggta ggtagcccca gggttgatag aaatatcgtc ctggggcttc atctttccag
       61 acctcagagt taacctgaaa ccggaaagct tcaggcaccc gccgttatct tacccttcct
      121 cagcaaaata gctgtttatt tatacagtga atcctgttta ggacttttca cctgtattat
      181 cgcccatgcc agtaaaacga aatcgcccgc agccatctct gactgcgggc gaaagtgtaa
      241 agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact ccccgcacaa
      301 cggctacttc ttcggttcgt aagcgagaac agccttaaac tcaatattac gttccttcac
      361 cgtaaactca cacagcgagt ctccgaccag aagcgtaaat cccagcaccg ttaaacacag
      421 cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc catgcgaaga
      481 agggactacc atccaaattg ttcgggtttg gagtggcagc cccagggttg atagaaatat
      541 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaaccggaaa gcttcaggca
      601 cccgcagtta tcttaccctt ccttagcaaa atagctgttt atttatacag tgagtcctgt
      661 ttcggacttc tcatttaatc aactgaagct gaacccgttt ttttctgcat gcctcaatcg
      721 tcaaaattct tcgccgccag ccagctctgc cataacttgt cacattgcaa aagtagccaa
      781 attgcttcag tcaccatcag ttcaccatcg ccaaaaaaaa gaaacattcc agaacttccg
      841 catctcaccc ggccgctccc acttctatac tgaatagaaa acgccaatat aagagaaacc
      901 tatgccatta cccgattttc atgtttctga accttttacc ctcggtattg aactggaaat
      961 gcaggtggtt aatccgccgg gctatgactt aagccaggac tcatcaatgc tgattgacgc
     1021 ggttaaaaat cagatcacgg ccggagaggt aaagcacgat atcaccgaaa gtatgctgga
     1081 gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc gggcaatttt cagcgatgca
     1141 gaaagtcgta ttgcaggcag ccgcagacca tcatctggaa atttgcggcg gtggcacgca
     1201 cccgtttcag aaatggcagc gtcaggaggt atgcgataac gaacgctatc aacgaacgct
     1261 ggaaaacttt ggttatctca tccagcaggc gaccgttttt ggtcagcatg tccatgttgg
     1321 ttgcgccagt ggcgatgatg ccatttattt gctgcacggc ttgtcacggt ttgtgccgca
     1381 ctttatcgcc ctttccgccg cgtcgccata tatgcaagga acggatacgc gttttgcctc
     1441 ctcacgaccg aatatttttt ccgcctttcc cgataatggc ccgatgccgt gggtcagtaa
     1501 ctggcaacaa tttgaagccc tgtttcgctg cctgagttac accacgatga tcgacagcat
     1561 taaagatctg cactgggata ttcgccccag tcctcatttt ggcacggtgg aggttcgggt
     1621 aatggatacc ccgttaactc ttagccatgc ggtaaatatg gcaggattga ttcaggctac
     1681 cgcccactgg ttactgacgg aacgcccgtt taaacatcag gagaaagatt acctgctgta
     1741 taaattcaac cgtttccagg cctgccgcta tgggctggaa ggcgtcatta ccgatccgca
     1801 cactggcgat cgtcgaccat tgacggaaga caccttgcga ttgctggaaa aaatcgcccc
     1861 ttcggcacat aaaatgggtg catcgagtgc gattgaggcc ctgcatcacc aggtcgtcag
     1921 cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg
     1981 gctggtgaaa aagcattgtg agatctgggc tggtgactaa gccgggaatt gccttacaga
     2041 agaatatccc tgacaatggt gttttaactt acatttaaca atgttatgaa acacccttta
     2101 gaaaccttga ccaccgcagc aggcattttg ctgatggctt tcctctcttg cctgctgctg
     2161 cccgcccccg cactggggct gacgctggca caaaaactgg tgaccatgtt ccatctgatg
     2221 gatcttagcc agctttacac tttattgttt tgtctgtggt ttttagtgct gggcgctatt
     2281 gagtattttg ttctacgctt tatctggcga cgctggttct cgctggcgga ttaaacgtcg
     2341 attatcgccg aacttcgcgg catactttgc ttattctcct tcgcctaaag gaacatttat
     2401 ggataagcaa tcactgcacg aaacggcgaa acgcgtggct cttgagttac cctttgtcga
     2461 gctttgctgg ccttttggcc cggagttcga tgtttttaaa attggcggca agatttttat
     2521 gctgtcgtcg gagctgcgcg gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc
     2581 cctgctaaat cagcaaatat acccaagcat caagccaggg tatcacatga ataaaaagca
     2641 ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa
     2701 cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc gatcaaaaac gactgcgtcc
     2761 tgactcaacc gtaaatcaat agcgcatttt tctcgcttac catttcatct cgatccttgt
     2821 aatctgctgg cacgcaaaat tactttcaca tggagtcttt atggatatca tttctgtcgc
     2881 cttaaagcgt cattccacta aggcatttga tgccagcaaa aaacttaccc cggaacaggc
     2941 cgagcagatc aaaactctac tgcaatacag cccatccagc accaactccc agccatggca
     3001 ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt gccaaatccg ctgccgataa
     3061 ttacgtgttc aacgagcgta aaatgcttga tgcctcgcac gtcgtggtgt tctgtgcaaa
     3121 aaccgcgatg gacgatgcct ggctgaagct ggttgttgac caggaagatg ccgatggccg
     3181 ctttgccacg ccggaagcga aagccgcgaa cgataaaggt cgcaagttct tcgccgatat
     3241 gcaccgtaaa gatctgcatg atgatgcaga gtggatggca aaacaggttt atctcaacgt
     3301 cggcaacttc ctgctcggcg tggcggcgct gggtctggac gcggtaccca tcgaaggttt
     3361 tgacgccgcc atcctcgacg aagaatttgg tctgaaagag aaaggctaca ccagtctggt
     3421 agttgttccg gtgggtcatc acagcgttga agattttaac gctacgctgc cgaaatctcg
     3481 tctgccgcaa aacatcacct taaccgaagt gtaattctct cttgccgggc atctgcccgg
     3541 ctatttcccc tcagattctc ctgatttgca taaccctgtt tcagccgtca tcatagtctg
     3601 ctgttgtata aaggagacgt tatgcaggat ttaatatccc aggttgaaga tttagcgggt
     3661 attgagatcg atcacaccac ctcaatggtg atgattttcg gtattatttt tctgaccgcc
     3721 gtcgtggtgc atattttttt gcattgggtg gtactgcgga ccttcgaaaa acgcgccatc
     3781 gccagttcac ggctttggtt gcaaatcatt acccagaata aactcttcca ccgtttagct
     3841 tttaccctgc aggggattat cgtcaatatt caggcggtat tctggctaca aaaaggcacc
     3901 gaagcggcag atattctgac tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt
     3961 tcagtcttct cgttgctgga tgtgattttg aatctggcgc agaaattccc ggcggcatct
     4021 cagttaccgc tgaaagggat atttcagggg attaaactga tcggcgcgat tctggtcggc
     4081 attttgatga tctcgctgct gattggtcag tcgccagcga ttctgatcag cggtcttggt
     4141 gcaatggctg ccgtgctgat gttggtattt aaagatccga ttcttggtct ggtggcaggt
     4201 attcagcttt ccgcgaacga tatgctgaaa ctgggcgact ggctggagat gccgaaatac
     4261 ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg tcaaagtgcg taactgggac
     4321 aataccatta ccactattcc cacctggtct ctggtttctg actcctttaa aaactggagc
     4381 gggatgtcag catccggcgg gcgacgtatt aagcgcagta tcagtattga tgtcaccagt
     4441 attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt gttaaaacct
     4501 tatttaacca gccgccatca ggaaattaat gagtggaatc gccagcaagg ttctacggag
     4561 tcggtattaa acctgcgccg aatgaccaat attggaacct ttcgtgccta tctgaacgaa
     4621 tatctacgta accatccgcg gattcgtaaa gatatgacct taatggtacg ccaactggct
     4681 cctggcgata acggtttacc gctcgagatc tatgcgttta ccaacaccgt ggtgtggctg
     4741 gaatatgaaa gcattcaggc tgatatattc gatcacatat ttgcgattgt cgaagagttt
     4801 ggtctgcgac ttcatcagtc gccaaccggc aatgatattc gctctctggc gggtgcattt
     4861 aagcagtaat taaaaaaacc gctcttatcg aatggatgag agcggtttcg gatggttgac
     4921 atcattttgt cggatacggc gtgaatgcct tatttccgac gcagcgtttt aaatgctaca
     4981 aacaggaata caatccacac cggcagcaga atcgctgaca agcgcatatc atccatcgtg
     5041 cacatcagca gcaaaatcat gccgaggaag gcgatgcaga gatagttgcc gaacggataa
     5101 agcagcgcct taaactgtgt ttcacgtccc tgacgtcgca tcgccgcgcg aaaacgcaga
     5161 tgcgccaggc agatcataat ccagttcaac agcagcgttg ctaccaccag cgccatcagc
     5221 agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga agtgatggct
     5281 ccggaaagca tcagcgagtt aatcggcacg ccgcgacggc tgacgcgagt caaaaacttc
     5341 ggtgcattac cctgcacaga aaggccaaac agcatgcggc tgttagaata aaccccgctg
     5401 ttatacaccg ataacgatgc caccagaatg acgaagttca gcgcagaagc caccacgttg
     5461 ctgtcgagat tatggaaaat catcacaaat gggctactgt tggatttcac ttccacccac
     5521 ggatagagtg ccagtaaaac caccagtgaa ccgatataaa acagcaagat gcgatacacc
     5581 acctgattca ctgctttagg gatgcttttt tccggatcgc gcgcttcagc ggcagtaatc
     5641 ccaatcagct ccagaccgcc gaaagagaac ataattaccg ccagcgacaa aatcagccca
     5701 ttccagccgg tggcaaaaaa accaccgtaa cgccagaggt tatcgatact ggctttctcg
     5761 ccgccgtgac cagaaaacag cagccacagg ccaaagccga tcataccgat gatcgccagc
     5821 actttaatca gcgcaaacca gaattcggtt tcgccatata agcgcacgtt caccaggtta
     5881 acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac atccgggaac
     5941 cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag cacgaacatt
     6001 acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt ataggcaaag
     6061 tgggcaaatg aaccagatac cggctcctca accaccattt cgccaagctg gcgcataatc
     6121 aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc cgccatctga
     6181 atcgccggac caatgccaag aaacagacca gtaccaattg cgccacccag cgcaatcagt
     6241 tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga cgcagtatct
     6301 tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt gttgaggggc
     6361 accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta aaccgggtat
     6421 cctgctttta tttccgtacc cgatgtcggt gcagccacat cagcttatac gccgccggga
     6481 taataaacag cgatagcaaa ggcgcggtga tcatgccgcc aatcatcggc gcggcaatcc
     6541 ggctcatcac ctctgaacct gctcccgttc cccacagaat cggcagcaga ccagcgataa
     6601 tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac cgcgccgtga tacaacgcct
     6661 catccagctt ctgctcgctg aatgtttgcg gattattcaa cgacggctcg gcctctatgg
     6721 cgtgacgtaa atacatcagc atcaccacgc caaattcggc agcaaccccg gcaagggcaa
     6781 taaaaccggt acccgtcgcc acggaaagat gaaagcccat ccaccacagc aaccagatgc
     6841 cacccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc acccgacgga
     6901 acgccagata caacagcacg aagatgatca tcaacgtcat cggcaccatg agtttcagct
     6961 tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg gtgcccggtt
     7021 ttaactgcac tttttcagct atcgcttttt gcagatcgtg aaccaccgac accatgtcac
     7081 gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt ttcagcatcg
     7141 acggtccggt agagacttta acgtcagcca cgtctgccag ggtgatttgc tgcttcatcg
     7201 gcgtgaggat cagcaactgg cgcagtgcct gcgggctatc gcgccagctt tgcggataac
     7261 gcagattaat tggataacga gcaatccctt ccaccgtttc gccaaccatc gccccgccca
     7321 ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat accgtaacgc gcggcttttt
     7381 cacggttaat ctcaacgttg atataacgcc caccttccag acgctcagca agcgcagaag
     7441 ccacgcctgg caccgttcgc gccacttctt caatttgctc cgccatcgtg tcgatatccg
     7501 ccagcacagt gccggaaact ttaatgccaa tagggctttt aatgccagtc gagagcatat
     7561 cgatacggtt acgaattggc ggcacccaca gattcgccag ccccggcaga cgcacggtgt
     7621 tatccagttc ctcaatgatt ttgtccatcg tcatgccagg ccgccactga tcctgcggct
     7681 taagctggat agtcgtttct accatctcca gtggagctga atcggtggcg gtttccgctt
     7741 tcccggtttt gccaaatacc cgcgccactt ccggtacgct cataatcagc ttgtcggttt
     7801 tttgcagcat gctcgccgcc tctgctgcgg aaatccccgg cagcgtcgat ggcatataca
     7861 acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc aactttattg agcggccaga
     7921 gaaccgtcag caccgaaagc gctgccacca acagcgtggt tttcggccag tgcagtactt
     7981 tcagcaacag cggatgataa acacgaatca aaaagcgatt gagcgggtta ctgctttccg
     8041 gcggaatttt gccacggatc cagtagccca tcaggatcgg gatcactacg atcgccagta
     8101 gcgccgcgcc cgccatcgca taggttttgg tgaacgccag cgggccaaac agacgacctt
     8161 cctgcccttc cagggtgaag atcgggataa acgacagcgt gataatcagc agactgataa
     8221 acagcgccgg acccacttca acagatgcat cggtgatcac ctgccagcgc gttttattat
     8281 ccagcgtggc gtcaggatgc tggtgctgcc actcttccag ccgtttatgc gcattctcga
     8341 tcatgacgat agcggcatcg accatcgccc cgacggcaat cgcaatgcca cccaacgaca
     8401 taatattggc gttcagtccc tgaaagtgca tgacaataaa agcaatacac aaccccagcg
     8461 gcaacgaaat aatcgctacc agcgccgagc gcacatgcca gagaaacagc gcacagacta
     8521 ccgccaccac aataaactct tccagcaact tgccgctgag gttgtcaatg gcgcggtcaa
     8581 tgagctggct gcgatcgtat gttgtaacta tctccacacc ttccggcaga ctgcttttca
     8641 gcgtttccag tttgtccttc acggcggcga tcacttcacg ggcgtttttg ccggaacgca
     8701 ggatcaccac cccgcccgcc acttcgcctt cgccgttcag ttcggcaatg ccccggcgca
     8761 tctccgggcc aacctggacc ttcgcaacat cacgcagata aacgggcacg ccattttcac
     8821 tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg cagatagccg ctggcgcgca
     8881 ccatatattc cgcttccgcc agttcgatcg acgaaccgcc cgcttcctgg tttgaagcat
     8941 ccagcgcgct ttttacgtcg gcgagactga tgccatactg cgccaggcgc tgaggatcga
     9001 taaccacctg atactctttc accacaccgc ccaccgacgc cacttccgca acgtcaggga
     9061 tggtttttag ctcatatttg agaaaccagt cctgcaatga gcgtaaatcg gccagatcgt
     9121 gcttaccgct gcgatccacc agtgcatatt catagatcca gccaacaccc gtggcatctg
     9181 gccccagctc ggcgctgact cccgcaggca gcttaccctg tacctggttg aggtactcca
     9241 gcacccgcga gcgcgcccag tacggatcgg tgccatcttc gaaaatgaca tacacataag
     9301 agtcgccaaa ctgcgagaaa ccgcgcacag tcttcgcgcc aggcaccgac aacatggtgg
     9361 tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctgaccg ggatagctgg
     9421 ttttaataat cacctgtaca tcagagagat ccggcagtgc atccactggc gtgttaatga
     9481 tggtccaggt gccccagata ctcagaaaca acgcgcccat cagcaccaga aaacggttcg
     9541 ccaccgagcg acgaataatc cattcaatca ttggctattc cctcaatgcg catgggtagc
     9601 actttcagag cgcatccgct ccagtgcgcc agaaatattg gcttcagaat caatcaggaa
     9661 caggccgctg gaaaccacct tttcaccttc cgccagacca gagcgtaacg cggtgacgcc
     9721 ttgcgatgcc tggaaaacgg caacgcgttt cggtacaaag cgcccgtcag catcaacggt
     9781 aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga gcagcatcgg
     9841 ttcgctggcg gtattgagtt gcaaccaggc gttcatgccc ggttttagcg cctcgtcggc
     9901 gttgtcgact tccagacgca gttgcagcgt gcgggtcgca gcatccacgc caggtagcag
     9961 cgtccatttg cggatggtga gtgttttatc aggtcgcgcc ggaacggtga gcgtaaactg
    10021 cgaggcatct ttcaccagcc aggcgatgga ctccgggatc gcagcagtga cccacaccgg
    10081 gtccataccc tgaattttcg cgaccacgtt atctttggcg atattcattc ccgcgcgcag
    10141 atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag tctggatttt
    10201 ttgcgtggcg atcaggcggc gaatatcagc ctccggcatt cccgccagtc gcagtcgctc
    10261 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcagcagta aatactcact
    10321 ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc ggcgcgccct tttgcacttt
    10381 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcggcgcggg cctgcacaat
    10441 ggcatactga tactcgttgt aactgacatt cgccgggaaa ctctgggaaa aagtcagcgg
    10501 cccgcgcgtg acggtcgccg ttttcacccc cagattctgc gtctgagtcg ggtcaatgcg
    10561 cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
    10621 cggcgatttc cctggtttat cgaaccgcgt attgggatac atcgggtcgt accagaataa
    10681 gactttacgt tctgcggtcg acgttttttc tgcgggcggt tcctcctttg caaaccaggt
    10741 aaaacctgcc gcagaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
    10801 tttcatctca ttaaacccgg attactggct gactttaata tcctgtaata aagaaaggct
    10861 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcgc tcattttcgt
    10921 ctgcggggtg atggtaaagc gcatagtcat ctccggccag ttcacggcag caatcggatc
    10981 gtgatggatg gtgatttttt tgctttccag atcaacacct tttaccacgc cagtggcgct
    11041 aataaccttt ggttgtgctt cgctcatggt ttcatgatga tgttcgttag cctgggcatt
    11101 aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgtttttt tcatttattc
    11161 tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata taaagaaatt
    11221 tcgttaacct gacgggcata attcaggtca agtaaagttt gtcgggttgc aaataaagaa
    11281 cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa tgcccgcgcc
    11341 cgttgcaaag taatttgcag cgacgcaaga taacgctgct gggcgctgat ttgagcgttc
    11401 agggtttgac gtaatgccag cgcatctgcc acttctttaa aggcgttctg gactttctgt
    11461 tcataattca ccaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
    11521 cgtccggcat taaaaacggg gatctcaatt ttaggaataa aattccacat cccgctgctg
    11581 gcgttaaata atgacgacag atcgctgctg gcggttgata ttccgctggt caggctgata
    11641 gacgggaaaa atgccgcacg cgcagcacca atattggcat tagccgccat taacgcgtgt
    11701 tcagcttcca taatatcagg gcgctgcaat aagatttgcg acgacaagcc cgccggtaat
    11761 ttaacgcttt gcaggctgtc gctgtttact gtctgcgctt gcggcagctt gccgtagctt
    11821 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
    11881 tcgctgcggg tactttctat caccccgcga gcctgttcca gcgccagaac attgctgcta
    11941 ccggtcaaca gttgtttttc gacaaacgca tatgactgct gataattacg cagcgtttct
    12001 tcggctattt gcaattgcgc atacgccagt tgctgattga aatagctttg cgcgacatta
    12061 gaaaccagca gaatatgcac cgcgcgctga gcttcctcag tggcaaaata attttggcgc
    12121 tcggcttcgc tcatgttctt taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg
    12181 ccagtcgata actcccgcgt cgtggctgaa tcgcctttaa gattgccgct ccagctgccg
    12241 ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgc
    12301 gcttcctgca ctttcagcgc cgccatgcgc aaatcccggt tattcaccaa cgcctcgcta
    12361 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccgcgtt ctggtagtta
    12421 tctgctgcgt taaccagacc gttctggctg agtgagaact gctgcggcac gggcattgcc
    12481 ggacgctgat aatccggtgc cagtgaacaa ccggttagcg caagggccac acaaaatggc
    12541 agaagtttac aaggagacat aggctcataa tttctggtga ttttatgctg ccaactttac
    12601 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
    12661 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaatatga
    12721 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
    12781 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
    12841 ccggtgatta tgatctgata atcctcgata ttatgctgcc ggacgtgaac ggctgggata
    12901 tcgtgcgcat gttgcgttcc gccaataagg ggatgccgat tctgttgctt accgcgcttg
    12961 gcaccattga acatcgcgtc aaggggctgg agttgggggc ggatgactat ctggtgaagc
    13021 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct actgcggcgc ggagcggcgg
    13081 taattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
    13141 tcacccgcag cggcacgcgc atcactctga ccagtaaaga atttactctg ctggagtttt
    13201 tcctccgcca tcagggcgaa gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca
    13261 tgaattttga cagcgatacc aacgccattg atgtggcggt gaagcggctg cgcggcaaaa
    13321 tcgacaacga ctttgagcca aagttgattc agaccgtgcg cggcgtgggt tacatgctag
    13381 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgcctgacc
    13441 ttttttatca gcctcgccac catcgcggcg tttttcgcct ttgcatggat catgatccac
    13501 tcggtaaaag tgcattttgc cgagcaggat attaatgatt taaaggagat tagcgccacc
    13561 cttgaacggg tacttaatca ccctgacgaa acgcaagccc gacgcttaat gacgctggaa
    13621 gatatcgtca gtggttattc caatgtgttg atctccctgg caaatagcca cggtaaaacg
    13681 gtgtatcact cccccggtgc gccggatatc cgcgagttta cgcgtgacgc catacccgat
    13741 aaagacgcgc agggcggcga ggtgtatctc ctttccggcc cgacgatgat gatgccaggg
    13801 cacggtcacg ggcatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
    13861 ctggttgacg gcaaaccgat ttatacgctc tacatcgcgc tttcgatcga ttttcatctt
    13921 cattacatca atgatttgat gaataaactt attatgaccg catcggtaat cagcatcctg
    13981 atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
    14041 cgtcaaatcc agaatatcac ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg
    14101 cccattgagc tggaacagct ggtactgtcg ttcaaccata tgatcgagcg tattgaggat
    14161 gtctttaccc gacagtccaa tttctcagcg gatatcgccc acgaaattcg cacacctatt
    14221 actaatctca ttacgcaaac ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg
    14281 gaagatgtgc tctactctaa tctcgaagaa ctgacgcgaa tggcgaaaat ggtcagcgat
    14341 atgctgtttc tcgctcaggc cgataacaac cagctaatcc ccgaaaagaa aatgctcaac
    14401 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgttagcaga agatcgcggc
    14461 gtggagctgc ggtttgttgg cgacgagtgt caggttgcgg gcgatccgct gatgctgcgt
    14521 cgggcgttaa gcaacctgct ttctaatgcc ctgcgtaata cgccaaccgg agagacaatt
    14581 gttgtgcgct gccagacggt cgatcacctg gtgcaagtta ccgtcgaaaa ccccggtacg
    14641 cccattgcgc ccgaacactt accgcgattg tttgaccgtt tctaccgcgt tgatccttcc
    14701 cgccagcgaa aaggcgaagg tagcggcatt ggcctggcga tagtgaaatc gattgttgtc
    14761 gcgcataaag gcacagttgc ggtaacgtca gatgtgcggg ggacaaggtt tgttatcata
    14821 ttacccgctt aatccttcag caaacggcaa cttttataac cagtgtaaaa ataacatgcc
    14881 gcaatagtaa tacctattaa tgataaaaag ctgtcacaat tcataaaaaa ccttaatata
    14941 cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg
    15001 tttttgctac ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcattatgt
    15061 tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac
    15121 gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag
    15181 atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata
    15241 tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct
    15301 accccaacga ccccgatact cagcgtgatg ttgacgaagt gtgcgctcgc ttcccgaacg
    15361 tgcataaggt aatctgcgcg cgtcctggcc ccaccagcaa agccgactgt ctgaacaacg
    15421 tgctggacgc catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc
    15481 tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg
    15541 agcgtaaaga tctgattcag atcccagtgt atccgttcga acgcgaatgg acgcacttca
    15601 ccagcatgac ctacattgat gagttttcag aactgcatgg caaagatgtt ccggtgcgtg
    15661 aagccctcgc cggacaggtg cccagcgcag gcgtcggtac ctgtttcagc cgccgcgccg
    15721 tgaccgcact gttagctgac ggtgacggta ttgctttcga cgtgcagagt ctgactgaag
    15781 attacgacat tggcttccgc ctgaaagaga aaggtatgac ggaaattttt gtccgttttc
    15841 cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcat gcgcggacgt
    15901 caaacatgat ctgcgtgcgc aaatatttcc ccgatacctt ttcgactgcg gttcggcaaa
    15961 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccacaaa tggacctcca
    16021 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
    16081 gcttcctcgc gatgctggtg atgatacagc ttttgctgtt gctggcgtat gaaagtttgt
    16141 ggcccgatgc ctggcatttc ctttctattt tcagcggcag cgcatggtta atgactctgc
    16201 tgtggctaaa ctttggcttg atgattaacc gcatcgtgca gcgggtgatt ttcgttactg
    16261 gctactacgg cctgacgcag gggctgctat ctgtcctgcg tcttttctgg ggcaacctga
    16321 ttaacttcat ggccaactgg cgcgcgttaa aacaggtact tcaacacggc gatccacgtc
    16381 gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt gactggcgat acccgctcgt
    16441 tgcgtccgtt aggtcaaatc ctgctggaaa atcaggtcat cactgaagaa caactcgata
    16501 cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc
    16561 tgattagcgc cgagcaactg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat
    16621 ccatcgatgc ctggcagatc ccttcctcgc tgattgccga aatgccggcc tccgtggcgt
    16681 tgcattatgc ggtactgccg ctgcgtctgg acaatgatga gttaattgtc ggcagtgaag
    16741 atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt
    16801 acgtcattgt tctgcgggga caaattgtca ccggattacg ccactggtat gcacgccgac
    16861 gcggtcacga tccgcgggca atgttgtaca atgcagttca gcatcagtgg ctcacggaac
    16921 agcaggccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac
    16981 tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac
    17041 gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt
    17101 tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca
    17161 aagcaggttt aaacactgaa caggttgcac aactggagtc cgaaaatgaa ggagaataac
    17221 cttaatcgcg tcatcggatg gtctggttta ctgctgacat ctttattgag taccagcgca
    17281 ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt
    17341 gttatttatc cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc
    17401 gccatccgcg aatttgaata tatacaccag caggtgccgg ataatattcc gctgacttta
    17461 taccttgcgg aagcctatcg tcattttggt catgatgacc gggcgcggct gttgcttgag
    17521 gatcaactga aacgtcaccc aggagatgat cggcttgagc gcagtctggc ggctattcct
    17581 gttgaagtga aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgtgcgat
    17641 gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg gctggcacaa
    17701 ttacctgtcg ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa
    17761 acgctgcgaa ccgatctgct gcaacgggca atctacctga aacaatggtc ccaggcagat
    17821 acgctatata atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg
    17881 tttgacgtgc ttcttgccgg gcagttggat gatcggatcc tggcgctgca atcacagggg
    17941 atcttcaccg atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa
    18001 aaagcacgcc tccagcatta tctcattgaa aataagccgc tgtttaccac ggacgcacaa
    18061 gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttca ggcgttggcg
    18121 aattatacgg tgcagtttgc cgataaccgc cagtatgttg ttggcgtgac gctaccggtg
    18181 ctgttaaaag aaggtcagta cgacgcagcg caaaaactac tcgccaccct ccccgccaat
    18241 gaaatgcttg aggagcgtta tgccgttagc gtagcgaccc gtaacaaggc tgaagctctg
    18301 cgtctggcac gattgctgta tcagcaagaa ccggcaaatc ttacccgcct ggatcaactg
    18361 acctggcaac tgatgcagaa cgggcagtca cgcgaagctg ccgatttatt gctgcaacgc
    18421 tatcctttcc aggacgatgc gcgtgtcagc cagacgttaa tggcgcgact ggcgtctctg
    18481 ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg caattttatc gaaaccttta
    18541 ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgctga taattgcccg
    18601 tcaatagttc gcttgctggg cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt
    18661 ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc
    18721 gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt
    18781 gaggactacg ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat
    18841 gaggatctgc ttgctgccgc caataccgcc caggcggcag gaaatggcgc ggctcgcgat
    18901 cgctggttac aacaggcaga acaacgtgga ctgggaaaca atgccctcta ctggtggctg
    18961 catgcgcaac gttacattcc aggtcagccg gaactcgcac tgaacgatct cacgcgctca
    19021 atcaatatta cgccttctgc caacgcttac gttgcgcggg caacaattta tcgccaacgt
    19081 cataatatcc cggcggcggt gagtgatttg cgcgccgcgc tggaactgga accgaataat
    19141 agcaacaccc aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg
    19201 cgggaaatgc tcgaacaggc gcataaaggg ctaccggacg atccggcact tatccgacaa
    19261 ctggcctacg tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctg
    19321 gtgattgatg acattgataa tcaggcgctg ataaccccac tgaccccaga gcaaaatcag
    19381 caacgcttca atttccgccg tctgcatgag gaggtcggtc gccgctggac gttcagcttc
    19441 gattcttcta tcggcttgcg ttccggcgca atgagtaccg ctaacaataa tgtcggcggc
    19501 gcagcgccag ggaaaagcta tcgtagctac gggcaactgg aagccgagta ccgcctcgga
    19561 cgcaatatgc tgctggaagg cgacctgctc tcagtttata gccgcgtctt tgccgatacc
    19621 ggtgaaaacg gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg
    19681 aagccgctgc gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacgga
    19741 caaaatggcg catccgatac catgctgcgc gccagcgcct cattctttaa tggcggcaaa
    19801 tacagcgacg aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat
    19861 gcggcgcaat atatccgcca ggatattcag gcgtggacgg cagattatcg cgtaagctgg
    19921 catcagaagg tggcgaacgg acagactatt gagccttacg ctcacgttca ggacaacggt
    19981 tatcgtgata aaggcactca gggcgcgcag cttggcggtg tcggggtccg ctggaatatc
    20041 tggaccggcg agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgaatat
    20101 caacatacct ttaaggcgat taatcaacgt aacggagagc gcaacaacgc gtttctcacc
    20161 attggagtgc actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc
    20221 agcccttttt cctttgcgat gaaaggtatt atctggcaac cacagaaccg tgatagtcag
    20281 gttaccgata cccagtggca ggggctgatg agtcagttac gtttgcaagg cttcgatacc
    20341 ctggttttgc aatggacccg ttacggcgat gcattcaccc agccagaaca gcgcgcgtta
    20401 ttgtttaagc gtgccgcagc tgcgcaacag gctggcctga agctgattgt cgggctgaac
    20461 gccgatccgg aattttttat acaccagaaa cagtcctccg cagcgctgga aagctatctt
    20521 aatcgcctgc tggctgccga tctccagcaa gccagattat ggagcgccgt gcctggcgta
    20581 acgccggatg gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagcc
    20641 gcccgtcagc ctttgctaac atggttaaac aacgcgcagc ggttgattag cgatgtttca
    20701 gcaaaaccgg tttatatcag tagttttttc gccggaaaca tgtcgctaga tggctatcgc
    20761 caactgctgg aacaagttaa agcaaccggt gttaatgtct gggtacagga tggcagcggc
    20821 gtggataaac tgaccgctga acagcgtgaa cgttatctac aggccagcgc cgattgccaa
    20881 agttccgccc cagcaagcgg cattgtttat gaactgtttg tcgctggtaa aggtaaaacg
    20941 tttacagcga aaccgaaacc ggatgcagag atcgcctcgc tgttagcgaa acgttcctct
    21001 tgcggtaaag acacgctcta tttctctctg cgctatttgc ccgtcgcgca gagtatcctt
    21061 gagtattaat gattaaaggg agcaacttag cttgctcctt tccttttcac tttcctaatc
    21121 aaaatcctgg caagatgaat tgtttagcag aatttctgtt ttcattttat tcgtctgacg
    21181 tgttccaatt atttagcaaa actgcgacaa caaagtgatg gtgtaatttg taaaagggaa
    21241 caaataaatg gctcgccctg caggattcga acctgtagcc cacggcttag aaactccagg
    21301 aacgacattt taagtcaaca aattaccgcg ccatctctgc gttcacacgt cccactacct
    21361 caaaatatgt aaaaccttgc aagccattgc gaggccttat gtgtctcagt tttgttcctc
    21421 ttttttatac taaaaaacac agcaattgag gataaacctc atgctattat cgctaatatg
    21481 catctaaagg catggcactt gaatagataa aaactccaca aagaaaaaaa taattagccc
    21541 ataaaaccac aatgcaaaac acaaacagac aattaaacta aaaaactgac agtgcattgt
    21601 gacaaaaatt caatgttaaa caaaatataa acagtggggc aatatacaat tgactcatta
    21661 agttagatat aaaaaataca tactcaatca ttaaaacgat tgaatggaga ccttttatgc
    21721 gggcgaaact tctgggaata gtcctgacaa cccctattgc gatcagctct tttgcttcta
    21781 ccgagacttt atcgtttact cctgacaaca taaatgcgga cattagtctt ggaactctga
    21841 gcggaaaaac aaaagagcgt gtttatctag ccgaagaagg aggccgaaaa gtcagtcaac
    21901 ttgactggaa attcaataac gctgcaatta ttaaaggggc aattaattgg gatttgatgc
    21961 cccagatatc tatcggggct gctggctgga caacactcgg cagccgaggt ggcaatatgg
    22021 tcgatcggga ctggatggat tccagtaacc ccggaacctg gacggatgaa agtagacacc
    22081 ctgatacaca actcaattat gccaacgaat ttgatctgaa tatcaaaggc tggctcctca
    22141 acgaacccaa ttaccgcctg ggactcatgg ccggatatca ggaaagccgt tatagcttta
    22201 cagccagagg tggttcctat atctacagtt ctgaggaggg attcagagat gatatcggct
    22261 ccttcccgaa tggagaaaga gcaatcggct acaaacaacg ttttaaaatg ccctacattg
    22321 gcttgactgg aagttatcgt tatgaagatt ttgagctagg tggcacattt aaatacagcg
    22381 gctgggtgga agcatctgat aacgatgagc actatgaccc aggaaaaaga atcacttatc
    22441 gcagtaaagt caaagaccaa aattactatt ctgttgcagt caatgcaggt tattacgtaa
    22501 ctcctaatgc aaaagtttat attgaaggcg catggaatcg ggttacgaat aaaaaaggtg
    22561 atacttcact ttatgatcac aatgataaca cttctgacta cagcaaaaat ggtgcaggca
    22621 tagaaaacta taacttcatc actactgctg gtcttaagta caccttttaa caacgttaaa
    22681 tagattttct ccccggtggt attgtatagc cccggggaat ttgtttactg ttaaaagcaa
    22741 acaatatccc ctaaccttgt aagcatttaa cctgcgtcct ggcgtgtaac ctcttaccgc
    22801 aacacaagac tcaatatatt caaattcgtt atgccgtggc atcatttaac cccttgtaat
    22861 tcatcgctat aattgattta attcacaaat aaaactataa
//
LOCUS       DAZFRE010000080        22812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFRE010000080 DAZFRE010000000
VERSION     DAZFRE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.080"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(34..429)
                     /locus_tag="ACHKUI_003516"
     CDS             complement(34..429)
                     /locus_tag="ACHKUI_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO6599653.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMSIIAGLACVDGVFLEESLAQKAEYLWGYSADILVMGDDWAGKFDD
                     FSCICEVVYFPRTPSVSTTGIIEVIRGKA"
     gene            complement(432..866)
                     /gene="fliJ"
                     /locus_tag="ACHKUI_003517"
     CDS             complement(432..866)
                     /gene="fliJ"
                     /locus_tag="ACHKUI_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6599654.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSADAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQHESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(869..2209)
                     /gene="fliI"
                     /locus_tag="ACHKUI_003518"
     CDS             complement(869..2209)
                     /gene="fliI"
                     /locus_tag="ACHKUI_003518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6599655.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSPEHQQSARAMKQHYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMHEPASLELVQSRLQILFPNGKKAEGQ"
     gene            complement(2202..2912)
                     /gene="fliH"
                     /locus_tag="ACHKUI_003519"
     CDS             complement(2202..2912)
                     /gene="fliH"
                     /locus_tag="ACHKUI_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6599656.1"
                     /translation="MTIETLRERYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGCKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(2916..3926)
                     /locus_tag="ACHKUI_003520"
     CDS             complement(2916..3926)
                     /locus_tag="ACHKUI_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6599657.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            complement(3904..5550)
                     /gene="fliF"
                     /locus_tag="ACHKUI_003521"
     CDS             complement(3904..5550)
                     /gene="fliF"
                     /locus_tag="ACHKUI_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6599658.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVTAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMSNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(5555..5896)
                     /locus_tag="ACHKUI_003522"
     CDS             complement(5555..5896)
                     /locus_tag="ACHKUI_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6599659.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMYGVLGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(5911..6897)
                     /locus_tag="ACHKUI_003523"
     CDS             complement(5911..6897)
                     /locus_tag="ACHKUI_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO6599660.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLESTLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVTAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIFPDALGLPEIDVADHSELQWPVQPSVSVAETSDLG
                     QAGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            7284..8135
                     /locus_tag="ACHKUI_003524"
     CDS             7284..8135
                     /locus_tag="ACHKUI_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6599661.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSMPLTLN
                     VKIAELSLNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            8128..8499
                     /locus_tag="ACHKUI_003525"
     CDS             8128..8499
                     /locus_tag="ACHKUI_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6599662.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            8496..9248
                     /gene="fliP"
                     /locus_tag="ACHKUI_003526"
     CDS             8496..9248
                     /gene="fliP"
                     /locus_tag="ACHKUI_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6599663.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIELSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            9251..9523
                     /locus_tag="ACHKUI_003527"
     CDS             9251..9523
                     /locus_tag="ACHKUI_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO6599664.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIVQLHDLCIHLFTQAALLVH"
     gene            9525..10307
                     /gene="fliR"
                     /locus_tag="ACHKUI_003528"
     CDS             9525..10307
                     /gene="fliR"
                     /locus_tag="ACHKUI_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6599665.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            10297..11436
                     /gene="flhB"
                     /locus_tag="ACHKUI_003529"
     CDS             10297..11436
                     /gene="flhB"
                     /locus_tag="ACHKUI_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO6599666.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQQINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSNEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLGLQALPFKLAMNEGLSLYASVMRNFVILFIFFALLDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            11420..13513
                     /gene="flhA"
                     /locus_tag="ACHKUI_003530"
     CDS             11420..13513
                     /gene="flhA"
                     /locus_tag="ACHKUI_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6599667.1"
                     /translation="MAKTTKSFLAPLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGVGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFTALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            13592..13936
                     /locus_tag="ACHKUI_003531"
     CDS             13592..13936
                     /locus_tag="ACHKUI_003531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599668.1"
                     /translation="MIRRVAAHLAPVHNCRMRRERLIRPTGERGFVGMIRRVAAHLAP
                     MHNRRMRRERLIRPTDERGFVGMIRRVASHLASAYNCRMRRTCLIRPTGERGFVGMIR
                     RVCVASGIGAQL"
     gene            complement(13931..14428)
                     /gene="rayT"
                     /locus_tag="ACHKUI_003532"
     CDS             complement(13931..14428)
                     /gene="rayT"
                     /locus_tag="ACHKUI_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO6599669.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(14604..15353)
                     /gene="yafL"
                     /locus_tag="ACHKUI_003533"
     CDS             complement(14604..15353)
                     /gene="yafL"
                     /locus_tag="ACHKUI_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6599670.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15665..16405
                     /gene="dpaA"
                     /locus_tag="ACHKUI_003534"
     CDS             15665..16405
                     /gene="dpaA"
                     /locus_tag="ACHKUI_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6599671.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16376..17143)
                     /gene="yafJ"
                     /locus_tag="ACHKUI_003535"
     CDS             complement(16376..17143)
                     /gene="yafJ"
                     /locus_tag="ACHKUI_003535"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6599672.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNEAWQKIMPGEWRLFCLGECVV"
     gene            complement(17348..17926)
                     /gene="lpcA"
                     /locus_tag="ACHKUI_003536"
     CDS             complement(17348..17926)
                     /gene="lpcA"
                     /locus_tag="ACHKUI_003536"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6599673.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            18166..20610
                     /gene="fadE"
                     /locus_tag="ACHKUI_003537"
     CDS             18166..20610
                     /gene="fadE"
                     /locus_tag="ACHKUI_003537"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6599674.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(20653..21099)
                     /gene="ivy"
                     /locus_tag="ACHKUI_003538"
     CDS             complement(20653..21099)
                     /gene="ivy"
                     /locus_tag="ACHKUI_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6599675.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            21280..22050
                     /gene="yafV"
                     /locus_tag="ACHKUI_003539"
     CDS             21280..22050
                     /gene="yafV"
                     /locus_tag="ACHKUI_003539"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6599676.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVSWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(22101..22295)
                     /locus_tag="ACHKUI_003540"
                     /pseudo
     CDS             complement(22101..22295)
                     /locus_tag="ACHKUI_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(22321..>22812)
                     /locus_tag="ACHKUI_003541"
     CDS             complement(22321..>22812)
                     /locus_tag="ACHKUI_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6599677.1"
                     /translation="VMLKMSLYVIILLFSLQFSAAITGKESEVVSPLLMDVNPSLTME
                     NISELSTSSEPSQQGVFPVICTRLHPGSVMKRQLLTGWGPVFIIGDDPFSLRWMSEHL
                     EILKSLNALGLVVNVESVERMEVLQQRADGLLLLPVICDNFVQALQLNAYPVLITEME
                     ISQ"
BASE COUNT         5030 a         6155 c         6155 g         5472 t
ORIGIN      
        1 ttactttgag gctcaaaaat aaattttatt ttgtcatgct ttccccctaa tcacctcaat
       61 gatccccgtc gtcgataccg acggcgtacg gggaaaatag accacctcac agatacacga
      121 gaaatcgtca aacttccctg cccagtcatc acccatcacc aaaatatccg cactgtaccc
      181 ccacagatat tccgctttct gcgccagcga ctcctccaga aatacgccat ccacgcacgc
      241 cagcccggcg atgatcgaca tgcggtcatc ctgatggtaa accggtgctc tgcccttttt
      301 ggcgatattt aaggcgtcgg acgatacgcc gaccagcagc cgttcgccca gcgtgcgggc
      361 gcgttgtaaa aggcgcagat gaccgacatg aaacacatcg aaggtgccga aggtgatgac
      421 cgttttcatg gctactccgc taatttgtgc cgtagccagc actgaacggc gatatcatcc
      481 accgcttttt gcgctttcgc cgtggcactt tcatgctgct cgcggcgttt gtcatccagc
      541 gtcagggcca ccactttctc ttcgcacgcc gccgccgtca ggtttttctg cattcgcgta
      601 gccttgatgt tagccagcgt tttttcctgc tcctgccagg cgatcacctt gcgtaaggtg
      661 cctttgtagc cgctgacgtt tttcagcgat tccaccgacg ccgccgcgtc cgcagacgtt
      721 ttctccacca ggtagcctaa cgctttgata ttgttgtcat agcccgcgca gatctgtttt
      781 tgctttgcca gttcgacggt tttgtctttg accgatttat cccgcaggcg ctgcaactgc
      841 gccagagtgt cgatgatttg tcgcatggtt attgcccttc tgcctttttg ccgttgggaa
      901 agaggatttg caggcggctt tgcaccagtt cgagggaagc gggttcgtgc atctcctggc
      961 gcaaaaatcg ctcgatagcc gggaacattt tcaccgcttt gtcgacgctg gcgtcggcac
     1021 ctgccacgta gcccccgagc gggatcagcg gtttgatctc catataggcg gcgtagtgtt
     1081 gtttcatcgc acgggcggat tgctgatgtt ccgggctggt gacctggttc atgcagcggc
     1141 tgatcgactg gccgatatca atggccggat agtgccccgc ttccgccagt ttgcgggtta
     1201 atacgatatg tccgtccagc actgcgcggg cgcagtcgac tattggatct tgctggtcgt
     1261 cgccttccgc cagcacggtg tagatggcgg tcatcgatcc tgagctttcg ctgttgcctg
     1321 cgctctcgac cagtttgggg atcatgccga atgccgacgg cggatagcct ttggtggcgg
     1381 gcggttcacc gagtgagagg gcgatttccc gctgcgccat cgcgtagcgg gtcagggaat
     1441 ccaccagcag taaaacgtga tgcccgcggt cgcgaaacca ggcggcgatg gagtggcaca
     1501 gttcggtggc tttcaggcgc atcagcggcg attcatcggc aggcgcgaca acaacgatag
     1561 actttgccag cccgtccgcg cccagcgaat ggtcaataaa ctctttcact tcgcgcccgc
     1621 gttcgccaat caacccgacc accacaatgt cggctttggt ctggcgggtg atcatcccca
     1681 gcagcacgct tttcccgacg ccgctgcccg ccatcagccc gacacgctgg cctttgccga
     1741 tggtcagcag gccgttaatc gctttcacgc ccacgtccag cggttcgtcc actgaacggc
     1801 gggttaaggg attgaccgag ggtggcagcg gcggcagaag gtcgttgccg ttcaggcggc
     1861 ctttgccatc cagcggttcg ccgagaccgt taaccacgcg gcctaaccag ctttcgccaa
     1921 tcaggatgtc gtgggctttt tcttccggga agacgcgtgc gcctgccatc aggccgaccg
     1981 gctgtttaaa cggcattaag taggtgatgt cgcgattaaa gcccacggcc tgcgcgtcga
     2041 tcagcgtgtg attggcgctt tcgatgcggc acagttgccc ggtcattagc gggcagccga
     2101 cgctctccag cagaatgccg ttcaggcgca ccagccgccc ggcgacgcgg gccagcggga
     2161 tcgactccag cgagcgcagg gcgttatcga aagcagaaaa atcactcacc ctgcgactcc
     2221 ggcgttagcg tctcttgcag cgcggtcatg cactgttcaa ggcgatgctc acagccgata
     2281 tccagttcag atttgtcggt aatcactcgg cattcgcctg gcggcagatc cggcgaaggc
     2341 gtcagccccc attcgctgac tttttccggc accgcgtcgc gcaggcggtt aaactcttct
     2401 gtactcagca tcacttgcag ggtctccggc atcgccggga aggccgccag cgcctcttcc
     2461 accagcgcca gtaactgcgt cggttgcagc gccagttcgc agcggatcac ctgacgggtg
     2521 actttctcca ccagttgcag caaatcttcc cgctgtttac gttcaatatg cgcgaggaag
     2581 tcgttcactt taccggtgat ggcttccagc ggctgcgccg cctcaacaaa ctgtttgcat
     2641 ccttcctgct gcccggcgag gctgccttcg gtgtagccct gacgtcgccc ctcggcaaaa
     2701 ccttgttgat gaccttcgct aaagccctgc tcctgacctt cggtcattcc ctgagcaaac
     2761 cctttttgca gcccttcctg aaaaccgtcc atcaactgac gctggtaatc ggcggcgctg
     2821 atactggcag taagcggttc tccggtcacc gcacgctggc gcggcgggaa gcggtgcagg
     2881 cgatagcgct cgcgcagtgt ttcaatggtc atgaattact ccgccgtccg ttccgcaaag
     2941 agctgtagct gaatttcgcc cgcttcttcc agctcacggg cgatgcccat gatttcgcgg
     3001 cggatctgtt cgatacggct gaccggcacc ggacccagac gcgcggtgat cgcttccagt
     3061 tgctgcgcct gacgttttgg cattaccgcg taaatcgagc ggcgcagcag ggcttcggtg
     3121 cctttcagcg ccaccgccca gtcttccatc ggcacttcgt caagcagacg ccgacgcact
     3181 tcttcgttct ggcggctgag gataaagaag tcgtacatct catcctggat ctgccccaac
     3241 acctcttcgt cacgttcgcg catctggtcg aggatcacct gctgattgcc ctggaagcgg
     3301 ttgacgatat ccgccgcctg tttgatgccc ttcaccttcg agccgtgctc cgaaagcacc
     3361 gacaggccac gttcaatcag gcggtccagc tcatccacca cgtcgcggtt gacgtcgttg
     3421 agtttcgcca cccggtagag aatttcgttt tgcaccgatt cgttgagata agagagcacc
     3481 gctgccgaga tttccggcgt cagaaacgcg aggaacaccg cctgcaattg cagatgttct
     3541 tcggaaatca gcatcgccaa ctggcgtggt tccacccatt gcaggcgcgc catccgcgag
     3601 cggatctcat cgccatagat gccgttgatc acgctactgg cgatctcggt gcccagcgct
     3661 ttgttaagaa tgccctgcaa catggagcgt gatgcgccgt tgatcccgct ctgttcgcgg
     3721 aactcatcaa agaagttatt gatcaccttg cgcgccatgc tggttttgac gccggaaagc
     3781 cgcgccatgt tttcgctcag gcgcaccact tcttcgcggc tgagtttttg catcaccgtc
     3841 gccgccgcct cttcgcccag acacagcaac aggatcgccg cctgttccag gtagctgttg
     3901 ctcttatttg ttgtcaattc gctcattgct ggtgatccat tgtctgagaa cttcggctac
     3961 gcgatcggtg tcgctcatgg ccagtttttg caggaactcg agtttcactt cgaggccaga
     4021 actttgtgac ggcaggctgt catcgccggg tagcgacggc aggtcgacgt ttttgcgctc
     4081 ctcttccggc atggcaatcc acggttcact gacggcttcc ggttctggtg tgataaccgg
     4141 cgcacgtacc gccgtcagcc gtttcatcaa cggacgcacc acgaaaaaca gcagcaacag
     4201 cgccagcagg ccgcagccaa tcagccgcac ccaggccagt acgctgtcat ctttccacag
     4261 tggaataacc ggttcgacag gcaccgcctg cggtacaaag ttgagtaacg ataaggagag
     4321 attgtcacca cgctgggcgt cgatcccggc ggcattgttc agcaacgcgg tcagttgcgt
     4381 ggtttgctcc ggcttccagt ttttcagcgc cggagcgttt tgattgagta ccaccgccac
     4441 gttgaggcgt ttcaccgcga agccgggatg ctgaatatgc tggacgctgc ggtcgtaaga
     4501 gtagtcgcgc ttactttcgc tgtgtttgga aagcgcttcc ggcgagcggt tttcttccgt
     4561 accgttgctc atctgattcg ctgcaatcgg cggacggtta ctgagcgaac caggcacgcc
     4621 catcgccacc tgattggtgt cgctgtccag cacgctctct tcgcggttga ttttcggcgc
     4681 gtcaccgtag tgttcctggg tttcgtcgat ggtgctgagg tcgagatccg gcatcacact
     4741 gacgcggtaa ttccccgtgc caaccagcga atccagcacg ttcgccacgc tggcacgggt
     4801 tttgtcctga atatctttga ggatctgatc gcgtttacgg gtcgcggcag aaaccgcctc
     4861 gcctgcgcca atgccgtcgg tcagcagatt tcccgcctga tcgaccacgc taactttgct
     4921 ggcctgtagc cccggaatac tgccggacac cagatgcaca atggcggtca cctggtccat
     4981 attcagcttc gcgccgtaat gcaagcgcac caccaccgag gcgctgtttt gcggctcatc
     5041 gctgaccaca aacgagcttt cttcgttgag cgccagatgc acccgcgcgc tttccaccgc
     5101 gtccagcgac atgatgctct gcgccagctc cccttcgaga ctgcgtttat agcgcacgtt
     5161 ctgcacgaac tgactgctgc cgagcacttc gtctttgtcc atcagctcgt agccgctggg
     5221 caatatcgcc tgcacgcctt tcgcggcaag ggtcattcgc gttttcgaca gtgcatcttc
     5281 cggcaccaga atctgcccgc tctgcgggtc gatgcggtag gtgagctttt cgccgtccag
     5341 caccgtgaca atctgcgaca cgggcagatt ttcctggcgt ccgtagagcg aaacgtagcc
     5401 gcgattaccg gtccacagca cgctgacgat aatcgccgtc gccgccacgg caagccccac
     5461 tcccgccatc aacgcccagc gtttgttatc tgcaagacgc agtcgaaacg ccggaaacgc
     5521 ctgggttaac ttctttattt gtgcattcat agggttagat cgacatactc atcaggtcgt
     5581 taaacccggt ggcgatttta ttgcgcacct gcaccagcgc ggagaaagag agcgacgcct
     5641 gctggctggc gatcatcgca cctgccaggt catcactttt gcccgtgtcg acagccgttt
     5701 gctgctgctc cgccacctgc tggaactgat caacgtgacc aagcacgccg tacataatgc
     5761 ggttaaacga cacgtccggg tctgtggaac tgaaggtcat ggcgggtagc accggcgcct
     5821 gggcgttggc ctgcatccgc tgcacgtcct gcatgatttg cgcctgcatg gtgctgttga
     5881 ttgtggtaat gctcattttt aacttccggg tcaggagaaa acttcgatac cttgcttgcg
     5941 catggaggcc agccgatagc gcagtgcgcg cggggtaatg cctaacaggt cggcaatttt
     6001 gctgcggttg ccctgatatt tacgcatcag gtcggcgata tattgatact gggcgctacg
     6061 tccggcctgg cctaaatcgc tggtttccgc aacagatacg gagggctgaa caggccattg
     6121 cagttcactg tgatcggcaa cgtctatttc cggcaggcct aaggcatccg ggaaaatcac
     6181 gccgtcgcgg tttaagatca ttcctcgctg aatggcgttt tccagttggc gaacatttcc
     6241 gggccagcgg taattaagta gcgcccggcg ggtagattct gaaagggtga tatttttcac
     6301 cagcaccgtg gcatattttt taataaacga ctctgccagc ggaataatat cgttcagacg
     6361 ttcacgcagt ggcggcatgg ttataggaat aaccgccaga cgataataaa gatcttcacg
     6421 aaaacgtcca gcagtcacat cctgttccag atttttattg gtgcaggcaa tcaggcgaaa
     6481 attgagtttg atttgtcggt tgctgcctaa tcgctcaacc tgctgttcct gcaatacgcg
     6541 taatattttg gcctgtaatg ccagcggcat atcgccaatt tcatcgagca ataaggtgcc
     6601 gttattagcc agttccattt ttcccggcac gctggcaatt gcgccggtaa atgcaccttt
     6661 gtcgtagcca aataaggtcg attccagcat attttccggg atcgccgcac agttcacgcc
     6721 gatatagggc gcgttatcat tttcaccaaa ggctacggtg tgaatatatt tcgccacgca
     6781 ctctttcccc gcgccggttt cgccctgaat gagcaccggt acgttaaagg cggcgacacg
     6841 ctttgccaga gtaaaagcgt taatgctgga agcggcagtt gcaataagtt cagacatagg
     6901 gtgaacgata taagtagcga caataatcag gatgttctga agcgtatcac cggtgggatt
     6961 aacaagttcg aattaaacga taataaaaat attatttgtt tataaacaac tagttagcaa
     7021 tgtataccgg ataaggcgtt tacgccgcat ccggcaattg cgtgccgatg cctgatgcga
     7081 cgctgcgcgt cttatcaggc ctacagtttg tgcaatgttt tatgacgatg cccgatatct
     7141 cgcccgttaa taatttaatt attttttcac cctattaata atttgcccct ttcactcctc
     7201 ctctatttaa tacctcacct tgtgtcaccg ccggatttgc gtgcacaaaa tcgcccatac
     7261 cattacagca gagaaagtaa gccatgctga agtacagtaa aacgccaggc atctttaaat
     7321 tagaaggtaa tcggctcgga cgcccctacc atcgcctgcc gaccctgttt acggggaatt
     7381 ttgacgtgat tgattcgcat cttggcagct actttctcaa aaagcatcgc agcaatatca
     7441 ccctaaaaaa gattggctgc gaaatggata ttattaataa aaacgctgaa ctgatggttt
     7501 cccaggtcgg gcatctggcg tttgatattg atcgctcctt attgttaatg ctgctgggca
     7561 atttttatgg tctggagtcg tctctcgaag aggctaaagc ccataacggg ttgccgacca
     7621 aaaccgaaac acgattaaag aaccgtctgg cgctggatat ctgcacgctg atatttaatc
     7681 tgcaaacctc cggtattgcg ctgaaattaa aactggacag cagcacggta attacccact
     7741 gggcgtatca attaaccttt acccttgcgg gcgatgagga gagcagtttt cgtattttgc
     7801 tcgacgacgc ccacaccgat tttattttga atctgatccg ccacagcgag cacagtaaac
     7861 cgcagcaggt ggcgaaatcc gttaataaac ctgccctgat taaagaaatc atccgctcta
     7921 tgccgctgac cctgaacgtc aaaatcgccg agctgtcgct gaatgtcgcg gatctggcgc
     7981 agataaaagc gggggatatt ttacccatct cgctgggaga aacgttcccg gtggcgattg
     8041 ggcaatctga attatttagc gccctgattg tggaagacaa agacaaactg tttttgtcgg
     8101 agctggcggg aaaaaatgag aacaaccatg agtaagcaag aagatatttt agccggggat
     8161 tttggcctca ccgatgaagt tgcgcctgtc gccaaatccg ccgacgccga aacgctggtc
     8221 acccgcctgg aagaccgatt ttccgactcg atgacgctac tgaaacgcat tccggtgacc
     8281 ttaacgctgg aagtgtcgtc ggtggaaatc atgttagccg acctgcttaa catcgacgac
     8341 gacacggtga ttgaactgga taaactcgcc ggggagccgc tggatattaa agtgaacaat
     8401 atcctgctgg gcaaagcgga agtggtggtg gtcaacgaga agtacggcct gcgcgtgctg
     8461 gaatttaaca cccgcgatat caacgacctg gcaccatgaa acgccgtact caactgacgc
     8521 tgggcctggc gctgctggcg ctcgccccgc tggcgctggc ccagggtggc gatattgcgc
     8581 tgcttaacgt ggtgacccac ggcaacactc aggagtacag cgtcaaaatt caggtactga
     8641 ttctgatgac cctcgtcggt ttgctgccga cgatggtgct gatgatgacc tgctttacgc
     8701 gctttattat cgtgctgtcg ctgctgcgcc aggcgctggg gctgcaacaa acgccgccga
     8761 accgcattct gataggtatt gagctctcac tcaccctgct ggtaatgcgc cccatctggc
     8821 tcaatattta cgaccacgcg gtagtgccgt ttgaaaacga tcagattacc ctgaccgatg
     8881 cgttaagcac cgccgcgacg ccgctgaaac gctttatgct ggcgcagacc gacaaaaaag
     8941 cgatggcgca aatcatgacc atcggcggcg cgaagggcaa tgccgccgac caggatctct
     9001 ccatcgtggt gcccgcctat gtactgagcg agctgaaaac cgccttccag attggcttta
     9061 tgatttacat cccgtttctg gttatcgacc tgattgtcgc cagcgtattg atggcgatgg
     9121 ggatgatgat gctgtcgccg ctgattgttt cgctaccctt taaactgatg ctgtttgtgc
     9181 tgattgacgg ctggtcgctg accatcggca cgctcaccac cagcattcgc gggctggggc
     9241 tgggctgatt atgttgaccg tagacgtagc cgccgacatt gtcgccagcg gtattaaagt
     9301 agtgattttg ctggtttcgg tactggtggt gccaagcctg ctggtcggcc tgctggtgag
     9361 cgtgttccag gcggtgacgc agattaatga acagaccctg agctttttgc caaggctgat
     9421 tgtcacgctg gtggtgctgg gcgtgtgcgg aaaatggatg attgtccagt tgcatgacct
     9481 gtgcattcac ctgtttaccc aggccgccct gctggttcat taagatgcgc acaagcgacg
     9541 ttacgcaact gatggatctg gcgctggggc tgtggtttcc cttcgtgcgc attatggcgt
     9601 ttctgcgcta tatgccggtg ctggataaca gcgccctgac cgcgcgcgtg cggattattt
     9661 tgtcgctggc gctggcgatc atcatcacgc cgttgatccc ccatcccatt ccccacgacc
     9721 tgctttcgct caatagcctg atcctgacgg tagagcaaat tttatggggc atgttattcg
     9781 ggctgatgtt tcagtttctg tttctggcgc tacaactggc cgggcaaatt ctctcgttca
     9841 atatggggat gagcatggcg gtgatgaacg acccgagcag cggcgcatcg accaccgtgc
     9901 tggcggagct aattaacgtc tacgcgatcc tgctgttttt tgccatggac gggcatctgc
     9961 tgctggtaag cgtgctgtat aagggtttca cctactggcc gatcggcaac gcgctgcatc
    10021 cgcaaaccct gcgtaccatc gcactggcct ttagctgggt gctggcgtcg gcatcgctgc
    10081 tggcgctgcc gaccactttt atcatgctga ttgtgcaggg ctgctttggc ttgctcaacc
    10141 gtattgcgcc gccgttgaac cttttttctc ttggctttcc catcaacatg ctggcggggc
    10201 tggtctgctt cgccaccctg ctctacaacc tgccagatca ctatctgcat ctggcgaatt
    10261 ttgtgttgca gcaactcgac gcgctgaaag gtcactatgg cggatagcag cagcgaagaa
    10321 aaaacagaga aaccctcggc gcaaaagctg cgtaaagcgc gtgaagaggg gcagctaccg
    10381 cgctcgaagg atatggggct ggcagcgagc ctgtttgcgg cgtttgtggt gatctccagc
    10441 agtttcccgt ggtatgccga ttttgtgcgc gagagtttta tcagcgtgca tcagtacgcg
    10501 cagcagatta ataacccgca ggtgatcggc cagtttctgc gccatcacct gttaattctc
    10561 ggcaagttta tccttacgtt gctgccgatg cccgccgccg cactgctctc ctcactggtg
    10621 cccggcggct ggctgttttt gccgaaaaaa atcctcccgg atttcagcaa aatcagcccg
    10681 ttaaaaggca ttgggcgact gttttccaac gaacatctgg cggaaacggg gaagatgaca
    10741 gtgaagtcgg tggtggtgct ggtaatgctg tgggtaagcc tgcgcaataa ctttgccgcc
    10801 tttctcggcc tgcaggcgct gccgttcaaa ctggcgatga atgaggggtt atcgctctac
    10861 gccagcgtga tgcgcaactt tgtcatcctg tttatcttct ttgcgcttct cgacgtgccg
    10921 ctggcgaaag ccctgttcac caaagggctg aagatgacca aacaggagct gaaagaagag
    10981 tacaaaaatc aggaaggcaa gccggaagtg aaggcgcgcg tgcggcggct gcaacggcaa
    11041 ctggcgatgg ggcaaattcg caaagtggtg ccgaaagcca acgtggtgat caccaacccg
    11101 acccactacg ccgtggcgtt acagtatgac tcatcacgtg ccgccgcgcc gtttgtggtg
    11161 gcgaaaggca ccgatgaaat cgccctctat atccgccagg tcgccactga aaatcaggtg
    11221 gaagtggtcg agttcccgcg actggcgcgc tcggtttact acaccacgca ggtgaaccag
    11281 caaattccgt ttcagcttta ccgggccatc gcccacgttc tgacctacgt cctgcaaatg
    11341 aaacactggc gcgagggcgc acagccgcgc ccggccctga acagacatat ttccattccc
    11401 aaagaggtac ttaaactgga tggcgaaaac aactaaatcg ttcctcgcgc cgctgcgcgg
    11461 cggcaatctc ggcgtgccgt tggtgatcct gtgtattctg gcgatggtta ttctgccgct
    11521 gccgcctgcg ctgctggata ttctgttcac cttcaacatt gtgctggcgg tgatggtgct
    11581 gctggtggcg gtgtcagcga aaaggccgct ggaattcagc ctgttcccga ccattttgct
    11641 tatcaccacc ttaatgcgcc tgacgctcaa cgtcgcctcc acgcgcgtgg tgctgctaca
    11701 tggccatctc ggcgtgggcg cggcgggtaa ggtgattgag tcgtttggtc aggtggtgat
    11761 tggcggcaac tttgtcgtcg gtttcgtggt gtttatcatc ctgatgatca tcaactttat
    11821 tgtcgtcacc aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc
    11881 gatgcccggt aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc
    11941 gcaggcacaa acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcaatgga
    12001 cggggcatcg aagtttgtgc gcggggacgc catcgccggg atgatgattc tggcgatcaa
    12061 cctgatcggc ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt
    12121 ccagcaatat gtgctgatga ctatcggcga tgggctggtg gcgcaaatcc cttccctgct
    12181 gctctccacc gcggcggcga ttatcgtcac ccgcgtcagc gataacggtg atatcgccca
    12241 tgacgtgcgt aatcagttgc tggcaagccc gtcggttctc tacaccgcca ccgggatcat
    12301 gtttgtgctg gcggtggtgc cggggatgcc gcacctgccg tttttgctgt tcaccgccct
    12361 gcttggtttt accggctggc ggatgagcaa gcgcccacag gcagcggagg cggaagagaa
    12421 aagcctcgaa acgctaaccc gtacgatgac agaaaccagc gagcaacagg tcagttggga
    12481 aactattccg ctgatcgaac ctatcagttt aagcctcggc tacaaactgg tggcgctggt
    12541 ggataaagcg cagggcaacc cgctcaccca gcgtattcgc ggcgtacggc aggtgatttc
    12601 cgacggcaac ggcgtgctgc tgccggagat ccgcattcgg gaaaatttcc gcctcaagcc
    12661 cagccagtac gccattttca tcaacggcat taaggctgat gaagcggata ttccggcaga
    12721 taaactgatg gccctgccct ccagcgaaac ctacggcgag attgacggcg tactggggaa
    12781 tgacccggcg tacgggatgc cggtcacctg gattcagccc gcgcagaaag cgaaggcgct
    12841 gaatatgggg tatcaggtaa tcgacagcgc cagcgtaatt gccacccatg tgaataagat
    12901 tgtgcgcagc tatattcctg atttgtttaa ctatgacgat attactcagc tgcataaccg
    12961 tttgtcgtcg atggcaccgc gactggcgga agatttgagc gcagcgctca attacagcca
    13021 gttgctgaaa gtgtaccggg cgctactaac tgaaggcgtt tccctgcgcg atatcgtcac
    13081 catcgccacc gtgttggtcg ccagtagtgc ggtgactaaa gatcatattc tgctggcggc
    13141 cgatgtgcgc ctggcgctgc ggcgcagcat aacccatccg ttcgttcgca agcaggagct
    13201 gacggtgtat acgctgaata atgagctgga aaatctgctg accaacgtgg tgaatcaggc
    13261 gcagcagggc gggaaagtga tgcttgatag cgtgccagtg gatccgaata tgctcaacca
    13321 gttccagagc acgatgcctc aggtgaagga gcagatgaaa gcggcgggga aagatccggt
    13381 gctgctggtg ccgccgcagc tgcgcccctt gctggcgcgc tacgcaaggt tgtttgcgcc
    13441 ggggctgcat gtgctgtcgt ataacgaagt gccggatgag ctggagttga agataatggg
    13501 ggcgttgatg taggcatccg gcatcggtgc atattgccgg atgcggcgtg aacgccttat
    13561 ccggcctacg ggtgagcacg gttttgtcgg catgataaga cgcgtagcgg cgcatctggc
    13621 accggtgcac aactgccgga tgcggcgtga acgccttatc cggcctacgg gtgagcgcgg
    13681 ttttgtcggc atgataagac gcgtagcggc gcatctggca ccgatgcaca accgccggat
    13741 gcggcgtgaa cgccttatcc ggcctacgga tgagcgcggt tttgtcggca tgataagacg
    13801 cgtagcgtcg catctggcat cggcgtacaa ctgccggatg cggcgtacat gccttatccg
    13861 gcctacgggt gagcgcggtt ttgtaggcat gataagacgc gtctgcgtcg catcaggcat
    13921 cggcgcacag ctataaaatg atacgctccc cagcactaaa atccgttacg tctcccgccc
    13981 aatcgatggg atataaccct ttcgcaacat cgcggtgaaa cgttgagaat ggccaatcac
    14041 tcacttgttt tacccaacca tgttttactg gatttatata aatataatca acatgatggc
    14101 gatagtcttt ggtattgcgg atagcatgtt cccaaaaacg tggttgccag atatttttca
    14161 atccacaagc atgggtaaat tgctttttaa tttcccgcca gcgcgaggaa aaatcatcat
    14221 cgccttcagg taatgtccat atgcaatgca tatgctccgg caaaactacc caagcgttaa
    14281 tttcaaaagg tctgtctcgc ttaactttaa taataacgtt acggagcgtc tgaaactggg
    14341 tggtcaaaag ttggtttcga cgattctgta aatttaccgt gaaaaaccag gttcccccct
    14401 tgatgtaata acgacgatat tcagacataa cgactccttt ttctcaacgg taagaagaaa
    14461 aggaaaagtc ggcaatgatg ttgtgttaaa tcgggaaagc ggctcgcaaa aaatgcacag
    14521 atttgtaggc atgataagac gcgtagcgtc gcatcagaca ccggcgcaca accgccggat
    14581 gcggcgtgaa cgccttatcc ggcctacaaa atcgtctctt ccgtcaaaat ccgccgcgcg
    14641 cctaaaaagt gatcctgcca gaaaggttcg gctaaccgac ttacccgaat ggtttccccg
    14701 gtgcgcggtg actcgataaa ctgaccgtct ccaagatata cgcccatatg gtcggctatc
    14761 tcacgactgt ggatatggaa aaacagcaaa tctccccggc gcaggtcgtt attcgccaca
    14821 atcgttgccc gacggtagtg gtacatctca ttggccgtgc gcgggagctt agcctcaagg
    14881 atcttgttgt aggcataaaa aaccaacccg ctacagtcga agcctttatc aggccgcgtg
    14941 cccccccaga tataaggctt acctagctgc tgctccaggc ggtgaatcgc cacttcggtg
    15001 atgttatgta gatggtcgct gcttaagaaa tgattattct ccaccaacgc ctgccagcgt
    15061 ctgtcactcg ccttgagcgg cgcaggaaac cattctggat gctgttttat ctgctcccga
    15121 ttatgctggc gcagcgccct tctgcttttg gcattgcctt ccacaatata gctggcctgc
    15181 tttttcagat gggtttggta ctgttttaat aaacgcgcac ggttttgcat ccgctggcgg
    15241 gcggcgtagc taaaagagat atgtgatgtg gtggcgctgg cgagtaaagg gagcaaaacg
    15301 cataacgaaa acacgcctgg atgcagaaat ctgccgtgca agaacgaaga catgaaggac
    15361 ataaaccagc tgaatgaagg tgagaatttt aaaagaccgg aggagtaaac gggattatta
    15421 attgcttatt tgcatattac tattgcggtt tcataatctt aggaaataag aaataaatct
    15481 acaatccatg cgcttttcgg actcttgaaa actgcccctg tgctaaattt gtaaaccctg
    15541 cgccgacagg cttgcgtaaa actcttttgg tgataatgtg agccttccaa atccagacta
    15601 ttccgatttg ggattcattt tgccgtcctg gcgtgtgtaa ccgttttatc aaggaataag
    15661 cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg tttcgtttgc
    15721 cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata agcagcagtt
    15781 gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg atctctacgt
    15841 caaaatgggc gagcaatatc aactgctcga cagctataaa atctgtaaat attccggcgg
    15901 cttggggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt atagcgtcca
    15961 gcgtaatcag ttaaaaccag acagccgtta ctacaaggcg attaatatcg gtttccccaa
    16021 tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg atgatccacg gcgattgtgt
    16081 ttccatcggc tgctacgcga tgaccaatca gggtattgat gagatcttcc agttcgttac
    16141 tggtgcgctg gtttttggtc agccgagcgt gcaagtgagt atttacccgt tccgcatgac
    16201 cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg agcagctgaa
    16261 gccaggttac gactactttg agcaaacccg taagccaccg accgtttctg tggtcaatgg
    16321 ccgttacgtg gtcagcaagc cgttaagcca cgaagttgtg cagccacaac tggcatcaaa
    16381 ctacacactc cccgaggcaa aataagcgcc attcacctgg cataatcttt tgccaggctt
    16441 cattgcccgt cagcggctgt gtcgcaatca ccgtgaccac atcattcggt gtggtctgcg
    16501 agctgaagtc gatttccaca tcctgatcca gcaacgttgc cacgccaaac ggcgcgcggc
    16561 gggtgatcca gtgtaaatta gtcgagcaat acgccattac atagcgcccg tcagaaagca
    16621 gcatgttgaa aacgcccttc tgccgcagtt catccgccag tgaggcgata tatttaaata
    16681 ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc aggagccagc
    16741 aaaaggcttt ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt tccagtgatt
    16801 tgtagcccgt cagttgtcca ttatgggcat aagtccagtt acgcccccat aactcgcggg
    16861 taaacgggtg agtattttcc agcgccacct cgccccgatt agcctggcga atatgagcca
    16921 ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg gaattaaagc
    16981 tgggttgcgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta atgccccagc
    17041 catctttatg tggcccggtt ccaccaccac gctgtacaag tccggtgaaa ctaaagcaga
    17101 tatcggtagg gacgttggca ctcatcccga gcaattcgca catgggtaac ctccacaaca
    17161 gcgggagcgc atggcccccg gcgaagattg atttaagaat tttcaggtcg gatgtggcgt
    17221 gaacgcatta ttcggcctac ggttcgggtg cagattgtag gcatgataag acgcgtcagc
    17281 gtcgcatcag gcatcagagc acaaatgccg gatgcggcgt aaacgcctta tccggcctac
    17341 gccagactta cttaaccatc tctttttcaa ttaactggat caggatatgg atcactttaa
    17401 tgtggatctc ctgaatgcgg tcggcataac caaagtgcgg aacgcgaatt tcaatatccg
    17461 ccgtgccagc cattttgccg ccgtctttac ccgtcagggt gatcactttc atccccttct
    17521 cacgcgccgc tgcgatcgct ttgatcacgt ttgcagagtt accggaggtg gagatcccca
    17581 gcagtacatc gccttcgcga cctaccgctt caacgtaacg ggagaaaata tcattgaaac
    17641 caaaatcgtt accgacgcag gaaatatgac taacgtcaga aatggcaatc gccgggtagc
    17701 ccggacggtt ttcacggtag cgaccggtca actcttcggc aaagtgcatg gcgtcgcaat
    17761 gggagccgcc gttgccacag gaaagcactt tgccaccggc tttaaagctg tctgctaaca
    17821 ggaccgccgc gcgctgaatg gcgtgaatat tggcgtcatc ttttaaaaag ttagccagcg
    17881 tttccgccgc ttcgttcagt tcattacgaa taagatcctg gtacatgagg atatccttca
    17941 gcataaatgt aatagacaaa atgcagtgta ccggataccg ccaaaagcga gaagtacggg
    18001 caggtgctat gaccaggact ttttgacctg aagtgcggat aaaaacagca acaatgtgag
    18061 ctttgttgta attatattgt aaacacattg ctaaatgttt ttacatccac tacaaccata
    18121 tcatcacaag tggtcagacc tcctacaagt aaggggcttt tcgttatgat gattttgagt
    18181 attctcgcta cggttgtcct gctcggcgca ttgttctatc accgcgtgag cttatttatc
    18241 agcagtctga ttttgctcgc ctggacagcc gtcctcggcg ttgctggtct gtggtcggcg
    18301 tgggtactag tgcctctggc cattatcctc gtgccattta actttgcgcc tatgcgtaag
    18361 tcgatgattt ccgcaccggt atttcgcggt ttccgtaagg tgatgccgcc gatgtcgcgc
    18421 actgagaaag aagcgattga tgcgggcacc acctggtggg agggcgactt gttccagggc
    18481 aagccggact ggaaaaagct gcataactat ccgcagccgc gcctgaccgc tgaagagcaa
    18541 gcgtttctcg acggtccggt agaagaagcc tgccggatgg cgaatgattt ccagattact
    18601 catgagctgg cggacctgcc gccggagtta tgggcgtacc ttaaagagca tcgtttcttc
    18661 gcgatgatca tcaaaaaaga gtacggcggg ctggagttct cggcttatgc ccagtctcgc
    18721 gtgctgcaaa aactctccgg cgtgagcggg atcctggcga ttaccgtcgg cgtgccgaac
    18781 tcattaggcc cgggcgagct gctgcaacat tacggcactg acgagcagaa aaatcactat
    18841 ctgccgcgtc tggcgcgtgg tcaggagatc ccttgctttg cactgaccag ccccgaagcg
    18901 ggttccgatg cgggcgcgat tccggatacc gggattgtct gcatgggcga atggcagggc
    18961 cagcaggtgc tgggaatgcg tctgacctgg aacaaacgct acattacgct ggcaccgatt
    19021 gcgaccgtgc ttggtctggc gtttaaactc tccgacccgg agaaattact cggcggtgca
    19081 gaagatttag gtattacctg tgcgctgatc ccgaccacca cgccgggcgt ggaaattggt
    19141 cgtcgccact tcccgctgaa cgtaccgttc cagaacggac cgacgcgcgg taaagatgtg
    19201 ttcgtgccga tcgattacat catcggcggg ccgaaaatgg ccgggcaagg ctggcggatg
    19261 ctggtggagt gcctctcggt aggccgcggc atcaccctgc cttccaactc aaccggcggc
    19321 gtgaaatcgg tagcactggc aacgggtgcg tatgcgcaca ttcgccgtca gttcaaaatt
    19381 tctattggta agatggaagg gattgaagag ccgctggcgc gtattgccgg taatgcctat
    19441 gtaatggatg ctgcggcatc gctgattacc tacggcatta tgctcggtga aaaacccgcc
    19501 gtgctgtcgg ctatcgttaa gtatcactgt acccaccgcg ggcagcagtc gattattgat
    19561 gcgatggata tcactggtgg caaaggcatt atgctcgggc aaagcaactt cctggcccgt
    19621 gcttaccagg gggcaccgat tgccatcacc gttgaagggg caaatattct gacccgtagc
    19681 atgatgattt tcggtcaggg ggcgattcgt tgccatccgt acgtgctgga agagatggaa
    19741 gcggcgaaga acaatgacgt caacgcgttc gataaactcc tgtttaaaca tatcggtcac
    19801 gtcggtagca acaaagttcg cagcttctgg ctgggcctga cgcgcggttt aaccagcagc
    19861 acgccaaccg gcgatgccac taaacgctac tatcagcacc tgaaccgcct gagcgccaac
    19921 ctcgccctgc tttctgatgt ctcgatggct gtgctgggcg gcagcctgaa acgtcgcgag
    19981 cgtatctcgg cacgtctggg ggatatttta agccagcttt acctcgcctc tgccgttctg
    20041 aagcgttatg acgacgaagg ccgtaatgaa gccgatctgc cgctggtgca ctggggcgta
    20101 caggatgcgc tgtatcaggc agaacaggcg atggacgatt tactgcaaaa cttcccgaac
    20161 cgcgtggttg ccgggctgct gaatgtggtg atcttcccga ccggacgtca ttatctggca
    20221 ccttctgaca agctggatca taaggtggcg aagattttac aagtgccgaa cgccacccgt
    20281 tcccgcattg gtcgcggtca gtacctgacg ccgagcgagc ataacccggt tggtttactg
    20341 gaagaggcgc tagtggatgt gattgccgcc gacccgattc atcagcggat ctgtaaagag
    20401 ctgggtaaaa acctgccgtt tacccgtctg gatgaactgg cgcacaacgc gctggcgaag
    20461 gggctgattg ataaagatga agccgctatt ctggtgaaag ctgaagaaag ccgtctgcgc
    20521 agtattaacg ttgatgactt tgatccggaa gagctggcga cgaagccggt aaagttgccg
    20581 gagaaagtgc ggaaagttga agccgcgtaa ttgaataacg gagccgaaag gctccgtttc
    20641 tttatccgct aattatttaa aattaaagcc atccggatgg ttttccaggc tgccggtcaa
    20701 cgccgcgaac agcaccgttt taccatcaat cgaaagcgca tcgttcacat tcaaccaggt
    20761 gagtttctct tgcgacgttt tctcatcaat agtcgagaac agccccgtca tctgattaga
    20821 tttctcggac cacatcacag cgatacgttg cgagccacag tcatgcggtt tgcacgcgct
    20881 catcacctga tacgtctcat ctcccaatgt cacggtttgt gcgggagtat aagtaccgcc
    20941 tttcatcacc caggcaggca gcttatgccc ttgcaccatc tgattaaacg ccgctttggt
    21001 ggtttcgccc tttgcaagac tgctaatggt taaatcatcc tgcgccattg cactggtggc
    21061 gatgaccaga gcggcgactg tcgttattgc cttaaacatc attcctcccg ggcttatcct
    21121 gcccatgtga ttaactccct aaagcctggc gataacacga taatttgcaa atcagatatt
    21181 tccaatatca gcatttgtta tcaatttatt ctgcgctaag gcaactgcct gataaggtgt
    21241 tagagttata aaacccttcg cataaaggag ttgtacaccg tgcctggttt gaagattacg
    21301 cttttgcagc aaccactggt gtggatggat ggtcctgcca acctgcgcca ttttgatcgt
    21361 caactggaag gtattaccgg gcgcgatgtg atcgttctac cggagatgtt taccagcggc
    21421 tttgccatgg aagcggcagc ttcgtcgcta gcacaagatg acgtagtgag ctggatgacc
    21481 gctaaagcgc agcagtgcaa tgcgctgatt gcaggcagtg ttgcattaca aacggagtct
    21541 gattcggtta accgcttttt gctggttgag ccgggcggca cggtacattt ttatgataag
    21601 cgtcatctgt tccgcatggc agatgagcat ctgcattata aagcgggcaa tgagcgagtg
    21661 attgtggaat ggcgcggctg gcgtattttg ccgctggtgt gctacgactt acgttttccg
    21721 gtgtggtcgc gcaatctcaa cgattatgac ctcgccatct acgttgccaa ctggcctgca
    21781 ccgcgttctc tgcactggca ggcattgctg acagcccgcg cgattgagaa tcaggcgtat
    21841 gtagcgggat gtaatcgcgt cggcagcgat ggcaacggct gccattatcg cggtgacagc
    21901 cgggtgatta atccacaagg cgagattatc gctactgccg acgcgcatca ggcaacgcgc
    21961 attgatgcgg agctatcgat gatggcattg cgggaatata gagaaaagtt tccggcatgg
    22021 caggatgcgg atgagtttag gttgtggtga gtgggggttg tgtttagggc cggggagagt
    22081 cggggaagac tacgaaagcc cgctctcctg cgacgacttt tatcacagga gtgtggaagt
    22141 gcttttccat caatcgcaat gacgtctatg tcgtctgaag aatgacattc acgcatccgg
    22201 ttaataaagc attcgtgaaa ttttactgaa cggatacagg acataactct gattatggtg
    22261 tcgtgaacag gattaccatt ttcaaaatca ccatattatt agcagtaatt atgttattaa
    22321 tcattgtgat atttccatct ctgtgattaa taccggataa gcatttaatt gcagagcttg
    22381 cacaaaatta tcgcagataa ctggcaataa caataaccca tcagcccgct gctgtaatac
    22441 ctccattcgt tctactgatt caacattaac aaccaaacca agggcattta gcgatttaag
    22501 gatctccaga tgttcactca tccagcgcag agaaaaagga tcatcaccaa ttataaaaac
    22561 aggcccccag ccggttaata actgacgttt cattacggag ccaggatgaa gtcgagtaca
    22621 tattacagga aataccccct gttgcgatgg ttccgatgat gtggataatt cagaaatatt
    22681 ttccattgtt agtgaaggat taacatccat taataatggt gaaacaacct cactttcctt
    22741 gccagtaatg gctgcactaa actgaaggga aaataacaat attattacgt ataaactcat
    22801 ttttaacata ac
//
LOCUS       DAZFRE010000081        22061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFRE010000081 DAZFRE010000000
VERSION     DAZFRE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.081"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            56..1510
                     /gene="ybbW"
                     /locus_tag="ACHKUI_003542"
     CDS             56..1510
                     /gene="ybbW"
                     /locus_tag="ACHKUI_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6599678.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1570..2930
                     /gene="allB"
                     /locus_tag="ACHKUI_003543"
                     /pseudo
     CDS             1570..2930
                     /gene="allB"
                     /locus_tag="ACHKUI_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2986..4287
                     /gene="ybbY"
                     /locus_tag="ACHKUI_003544"
     CDS             2986..4287
                     /gene="ybbY"
                     /locus_tag="ACHKUI_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO6599679.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4309..5453
                     /gene="glxK"
                     /locus_tag="ACHKUI_003545"
                     /pseudo
     CDS             4309..5453
                     /gene="glxK"
                     /locus_tag="ACHKUI_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
     gene            complement(5582..6367)
                     /gene="allE"
                     /locus_tag="ACHKUI_003546"
     CDS             complement(5582..6367)
                     /gene="allE"
                     /locus_tag="ACHKUI_003546"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6599680.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPLGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6378..7613)
                     /gene="allC"
                     /locus_tag="ACHKUI_003547"
     CDS             complement(6378..7613)
                     /gene="allC"
                     /locus_tag="ACHKUI_003547"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6599681.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7635..8684)
                     /gene="allD"
                     /locus_tag="ACHKUI_003548"
     CDS             complement(7635..8684)
                     /gene="allD"
                     /locus_tag="ACHKUI_003548"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6599682.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            9001..10668
                     /gene="fdrA"
                     /locus_tag="ACHKUI_003549"
     CDS             9001..10668
                     /gene="fdrA"
                     /locus_tag="ACHKUI_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6599683.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10678..11937
                     /gene="ylbE"
                     /locus_tag="ACHKUI_003550"
     CDS             10678..11937
                     /gene="ylbE"
                     /locus_tag="ACHKUI_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6599684.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQYIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDVGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11948..12763
                     /gene="ylbF"
                     /locus_tag="ACHKUI_003551"
     CDS             11948..12763
                     /gene="ylbF"
                     /locus_tag="ACHKUI_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6599685.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALATSDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12760..13653
                     /gene="ybcF"
                     /locus_tag="ACHKUI_003552"
     CDS             12760..13653
                     /gene="ybcF"
                     /locus_tag="ACHKUI_003552"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6599686.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13986..15053)
                     /gene="purK"
                     /locus_tag="ACHKUI_003553"
     CDS             complement(13986..15053)
                     /gene="purK"
                     /locus_tag="ACHKUI_003553"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6599687.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(15050..15559)
                     /gene="purE"
                     /locus_tag="ACHKUI_003554"
     CDS             complement(15050..15559)
                     /gene="purE"
                     /locus_tag="ACHKUI_003554"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6599688.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15655..15984)
                     /locus_tag="ACHKUI_003555"
     CDS             complement(15655..15984)
                     /locus_tag="ACHKUI_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599689.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFANSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(16095..16817)
                     /gene="lpxH"
                     /locus_tag="ACHKUI_003556"
     CDS             complement(16095..16817)
                     /gene="lpxH"
                     /locus_tag="ACHKUI_003556"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6599690.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16820..17314)
                     /gene="ppiB"
                     /locus_tag="ACHKUI_003557"
     CDS             complement(16820..17314)
                     /gene="ppiB"
                     /locus_tag="ACHKUI_003557"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6599691.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            17488..18873
                     /gene="cysS"
                     /locus_tag="ACHKUI_003558"
     CDS             17488..18873
                     /gene="cysS"
                     /locus_tag="ACHKUI_003558"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6599692.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18909..19430)
                     /gene="ybcI"
                     /locus_tag="ACHKUI_003559"
     CDS             complement(18909..19430)
                     /gene="ybcI"
                     /locus_tag="ACHKUI_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6599693.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(19538..19750)
                     /gene="ybcJ"
                     /locus_tag="ACHKUI_003560"
     CDS             complement(19538..19750)
                     /gene="ybcJ"
                     /locus_tag="ACHKUI_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6599694.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19752..20618)
                     /gene="folD"
                     /locus_tag="ACHKUI_003561"
     CDS             complement(19752..20618)
                     /gene="folD"
                     /locus_tag="ACHKUI_003561"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6599695.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            20668..20856
                     /locus_tag="ACHKUI_003562"
     CDS             20668..20856
                     /locus_tag="ACHKUI_003562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599696.1"
                     /translation="MSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYIRCGR
                     TLVQQLVQKPLTQQGLTV"
     gene            20889..20965
                     /locus_tag="ACHKUI_003563"
     tRNA            20889..20965
                     /locus_tag="ACHKUI_003563"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20923..20925,aa:Arg,seq:tct)
     gene            complement(20981..21582)
                     /locus_tag="ACHKUI_003564"
                     /pseudo
     CDS             complement(20981..21582)
                     /locus_tag="ACHKUI_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            21589..21963
                     /locus_tag="ACHKUI_003565"
                     /pseudo
     CDS             21589..21963
                     /locus_tag="ACHKUI_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         5077 a         5545 c         6002 g         5437 t
ORIGIN      
        1 tcctcatagc tagattaaaa caacgttatt cgatacgtga aattaagagg gatttatgga
       61 acatcagaga aaactattcc agcaacgcgg ctatagcgaa gatctactgc cgaaaacgca
      121 aagtcagcgg acctggaaaa catttaacta ttttacctta tggatgggtt cggttcataa
      181 cgttcccaat tatgtgatgg ttggcggctt ttttattctc ggcctgtcta cctttagtat
      241 tatgctggca attatcctca gtgccttttt cattgccgcg gtaatggtat taaacggtgc
      301 tgcgggcagt aaatacggcg taccgtttgc catgatcctg cgtgcttctt acggcgtacg
      361 tggtgcactg tttcccggat tattaagagg cggtattgct gccattatgt ggtttggcct
      421 gcaatgttac gcggggtcac tggcctgctt gattctgatt ggcaaaatct ggccgggatt
      481 tttgactctc ggtggcgatt tcacgctgtt aggcctttct ctaccgggct taattacttt
      541 tttactcttc tggctggtta acgttggtat tggttttggc ggcggaaaag ttttaaataa
      601 attcactgcc attcttaacc cgtgcatcta tatcgttttc ggcggtatgg cgatttgggc
      661 gatttcgctg gtcgggatcg gtccaatctt tgactacatt ccgggcggta ttcagaaagc
      721 agaaaacagt ggcttcctgt ttctggtggt gataaacgcg gtggttgcgg tctgggcggc
      781 accggcggtg agcgcatccg actttacgca aaacgcccac tcgtttcgtg agcaggcgct
      841 ggggcaaacg ctgggtttag ttgtggccta tattctgttt gcggtcgccg gggtatgtat
      901 tattgccgga gccagtattc attacggcgc tgacacctgg aacgtgctgg atattgttca
      961 gcgttgggac agcctgttcg cctcgttctt tgcggtactg gttattctga tgacgactat
     1021 ctctactaac gccaccggta atattattcc ggctggttat cagattgccg ccattgcacc
     1081 gacgaaactg acctataaaa acggcgtact gattgccagt attatcagcc tgctgatctg
     1141 cccgtggaaa ttaatggaaa atcaggacag tatttatctg ttcctcgata ttatcggcgg
     1201 aatgcttggt ccggtaattg gtgtgatgat ggcgcattat tttgtggtga tgcgtgggca
     1261 aattaatctt gatgaactgt acaccgcacc tggcgattat aaatattacg ataacggttt
     1321 taacctcacc gcgttttcag taactctggt ggccgttatt ttatctcttg gcggtaagtt
     1381 tattcccttt atggaaccgt tatcacgtgt ttcatggttt gtcggcgtca tcgtcgcctt
     1441 tgcggcctac gccttattaa agaaacgtac aacagcagaa aaaacaggag aacaaaaaac
     1501 cataggttaa ttaatcacga tattgaacat tgagttaaaa accaatctgt attttacaag
     1561 gagtttgtta tgtcttttga tttaatcatt aaaaacggca ccgttatttt agaaaacgaa
     1621 gctcgcgttg tagatatcgc cgttaaagac ggaaaaattg ctgctatcgg ccaggatctg
     1681 ggcgatgcaa aagacgttat ggatgcgtct ggtctggtgg tttcgccggg catggttgat
     1741 gcgcacaccc atatttctga accgggtcgc agccactggg aaggttatga aaccggtact
     1801 cgcgcagcag caaaaggtgg tatcaccacc atgatcgaaa tgccgctcaa ccagctgcct
     1861 gcaacggttg accgcgcctc aattgaactg aagttcgatg ccgctaaagg aaagctgact
     1921 atcgatgcgg cacaactcgg tggcctggtg tcttacaaca ttgaccgtct gcatgagttg
     1981 gatgaagtgg gtgttgtcgg tttcaaatgc ttcgttgcga cctgtggcga tcgcggtatc
     2041 gacaacgact tccgtgacgt caatgactgg caattcttca aaggcgcgca gaagctgggt
     2101 gaactaggcc agccggtgct ggtgcactgc gaaaacgcgc tgatctgtga cgcactgggc
     2161 gaagaagcga agcgtgaagg tcgcgtaact gcccatgact atgtggcttc gcgtccggta
     2221 tttaccgaag tggaagcgat tcgccgcgta ctgtacctgg cgaaagtcgc tggttgccgt
     2281 ctgcacgttt gccacgtcag cagcccggaa ggtgttgagg aagtgactcg tgcacgtcag
     2341 gaaggtcagg atgttacttg tgaatcctgc ccgcattact ttgtgctgga taccgatcag
     2401 ttcgaagaaa tcggtactct ggcgaagtgt tcaccgccga tccgcgatct ggaaaaccag
     2461 aaaggcatgt gggaaaaact gtttaacggt gaaatagact gcctggtttc cgaccactct
     2521 ccatgcccgc cggaaatgaa agccggtaac atcatgaaag cgtggggcgg tatcgctggt
     2581 ctgcaaagct gcatggacgt gatgttcgat gaagcggtac agaaacgcgg aatgtctctg
     2641 ccaatgttcg gcaaattaat ggcgactaac gcagcagata ttttcggtct gcagcaaaaa
     2701 ggccgtatcg cccaggaaaa gatgccgact tcgtcttcat tcagccgaat agcagctatg
     2761 ttcttaccaa tgacgatctg gaatatcgcc acaaagtcag cccgtatgtt ggccgtacta
     2821 ttggcgcgcg tatcacgaaa accatcttac gtggtgatgt gatttacgat atcgaacagg
     2881 gcttccctgt tgcgccgaaa ggtcaattta tccttaaaca tcagcagtaa tctggccctg
     2941 caatgcccgt ccttgtggcg ggcattctcc ggttaaggtg tgtttatgtt caattttgca
     3001 gtgggccgcg aaagcctgtt atcaggattc cagtggtttt tctttatttt ttgcaacacg
     3061 gttgtggttc ctcctacgct actttctgct tttcagttgc cgcaaagtag cctgcttacg
     3121 ctcacgcaat atgcttttct tgctaccgca ctggcctgct tcgctcaggc gttctgcggt
     3181 catcgtcgcg ctattatgga ggggccgggc ggcctgtggt ggggaaccat cctgactatc
     3241 acccttggcg aagcatcgcg cgggacaccg atcaacgata tcgctaccag tctggcggtg
     3301 gggattgcgc tctccggcgt gctgacgatg ttgattggtt ttagcggatt aggccatcgc
     3361 ctggcacggt tatttacgcc gtcggtgatg gtcttgttta tgttgatgct gggtgcgcaa
     3421 ctgaccacta tctttttcaa aggtatgctc gggctgccgt ttggcatagc cgacccgaat
     3481 tttaaaattc aattaccgcc gttcgcgctc tcggtggcgg tgatgtgcct ggtactggcg
     3541 atgattattt tcctgccaca acgttttgcc cgttacggcc tgctggtcgg tacgataacc
     3601 ggctggttgt tgtggtactt ttgctttcct tcttcgcatt cgctctccgg tgagttgcac
     3661 tggcagtggt tcccgctcgg cagtggcggt gctttgtcgc cggggattat tctgacggcg
     3721 gtgattacag gtctggtaaa tatcagcaat acctacggtg cgattcgggg cacggatgtt
     3781 ttttatccgc agcagggcgc ggggaatacg cgttatcgtc gcagctttgt ggcgaccgga
     3841 tttatgacac tgataaccgt gccgctggcg gtaattccat tttcaccgtt tgtttcatcc
     3901 attggtttat taacccagac tggcgattac acgcggcgtt cgtttattta tggcagcgtt
     3961 atttgcctgc tggtggcgct ggttccggca ctcacgcgac tgttttgcag tatcccttta
     4021 cccgtgagta gtgcggtcat gctggtttct tatctgcctt tactcttttc cgcgctggtg
     4081 tttagccagc aaataacgtt taccgctcgc aatatttatc gactcgcatt gccgttattt
     4141 gtcggcatat ttttaatggc attaccgcct gtgtatctgc aagaccttcc attaacgctt
     4201 cgtcctctgc tcagtaacgg cttactggtc ggaattttac tggctgttct tatggataat
     4261 cttattccgt gggaacgcat cgaataattt gttgaaaaag gattgataat gaagattgtc
     4321 attgcgccag actcttttaa agagagctta agtgcagaaa aatgttgtca ggcaattaaa
     4381 gccgggtttt cgaccatatt tcccgatgcg cactatatct gtttgccgat agcggatggc
     4441 ggcgaaggga cggtggaggc gatggtcgcc gcgacggacg gtaacatcgt gaagcttgaa
     4501 gtctgcgggc cgatgggcga aaaagtgaat gctttttatg gccttaccgg cgacgggaaa
     4561 acggcggtga ttgagatggc agcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt
     4621 aatccgttgc tggcctccag ttttggtacg ggggagttaa ttcgccatgc gctggataat
     4681 ggcattcgcc atattattct cggcattggc ggcagtgcga cggtcgacgg cggtatgggc
     4741 atggcgcagg cgctcggtgt gcgtttcctt gatgccgacg gtcaggcgct ggcggctaac
     4801 ggcggcaatt tagcgcgcgt ggcaagcatt gagatgaatg aatgcgatcc gcgtctggcg
     4861 aactgtcata ttgaagtagc atgtgacgtt gataacccgc tggtaggggc acgcggcgcg
     4921 gcggcagtgt ttggcccgca aaaaggggca acgccggaga tggtcgaaga gctggaacag
     4981 ggctgcaaaa ttacgcccgt gttttacaac agctaactga aatcaacgtc tgccagatgg
     5041 cgggtggtgg cgcggcgggc ggtatgggca ttgcggcggc ggtattcctc aatgcggata
     5101 ttaaaccggg tattgagatt gtgttgaatg cggtcaatct tgcgcaggca gtgcagggtg
     5161 cagcactggt gatcaccggg gaagggcgca tcgactcgca aacggcaggc ggtaaagcgc
     5221 cgctgggcgt ggcgtcggtg gcgaagcagt ttaatgtccc ggtgattggg attgctggcg
     5281 tattgggtga tggcgtggaa gtggtgcacc agtacggcat tgatgcggta ttcagcattt
     5341 tgccacgtct ggcaccttta gccgaagtgc tcgccagcgg tgaaaccaat ctcttcaaca
     5401 gtgcgcgaaa tattgcctgc gccattaaaa taggtcaggg aattaaaaac tgactattac
     5461 ctttaaagcg gatgcgattt atatcgcatc tttttttacc tgaattgact acaaaaaaag
     5521 ccacggtata aaccgtggca aaatccaaca tagctaaaaa taatcaggcg agtggtatga
     5581 cttaaatctc tacgtcgcgg ttgcaatctt tcgagtaaat atagctgaac gcttcaccac
     5641 gccctacgcc ataaccagcc tgtaaagaat aagcgcccat aaagatgtaa tcgccttttt
     5701 tcaccgggat ccagttattg tcgaggttat aaaccccctg accggaaaga atataggcac
     5761 cgtgttcctg aacgtgggtt tcgatataac cgtggctggc acctggtgca aaagagagga
     5821 tatgcatgtt catatcaaaa cctaactctt tgggcagaaa atccagcaga ataacatcgt
     5881 ccatgccttc ataatgaatg cgttccagtt cgctggcatt gccagaaacc agccacggcg
     5941 catgaccttc taccggaata tagcgacgct tatataaaaa gatttggctg tcttcggcct
     6001 gggcgttaac aaacgtcatt aaggagccta gcgggcaata aagatagcca ccttcactta
     6061 aggtaaatgt tttcccttcg gctttggcag tgatatttcc agagatcaca tacaggaacg
     6121 tttcaatgcc ttcgccaccg aagccctgtt ggttgccgcc gttttgatgc agtgtgacca
     6181 gataatcaac aaaagaggca cccagctttg gcgtggagag gattgtcgcg tcacaatttt
     6241 caaagcccgg aataatattt tttaccagac cgtctggtgt taacagtgcg aaattaccgt
     6301 gtttcacaat cgcacggtta gccagtaaat catcgcggta accggtgacg ttatttaaat
     6361 atcccattta taactcctta tttctgccag gcaagttgat aaagcatgag tgccaacgtt
     6421 ttgacccctt cggcaaggtc ggtaatattg gtgcgttccg ccgggttatg gctgatcccg
     6481 ttgatgctgg gaatgaaaat catgcaggtc ggcacgcgag gcgcgaaaat ttgcgcgtcg
     6541 tgcccggcac cactgtgcat cacccggtaa ttcagttttt cgctttcaca caattctgtc
     6601 agggtggcga ccaactcctt attcatcggc acgggttctt cgtccatcca taaatcgata
     6661 tcaataccaa tatccatttc atcgcaaatc gcccgcatgt cgttttctaa ctgttgggtg
     6721 aaatcgcgca gcacggtagc gtcggtatga cgacaatcaa tggtgaacgt ggttttaccc
     6781 ggcactacat tcaccgtatt cgggcgcggc tctactttgc caaaggtcag aaccagcggg
     6841 tcgcccatct ttttcgcttt ttcgacagac tgatggcaaa tgcgactgaa agcgtaaacc
     6901 gtatcacgac gatagcccat cggcgtggtg cctgcatggt ttgattcacc gttcagcgtc
     6961 accgtataac gacgctgccc gacaattgca ttcaccacgc caattgattg cccattactt
     7021 tccagcacac agccctgttc gatatgcagt tcgacaaagg ctttaatatc ctgacgcgga
     7081 gttagtgggg cgttcggcag agtaaatccg caagccttca tcgcatcgac aaaactattt
     7141 cctttggcat cacagatatt ccgcacgtcg tcaggattcg ccagcccaaa aatattttta
     7201 ctgccccaga aaacatacgg gaagcggctg ccttcttctt ctgccatcgc caccacttcg
     7261 accgtacgta acggcgcgcc gtattgcgtt ttcagccagt caatagccag ccacgccgcc
     7321 agcgcaccga attgcccgtc gaggttaccg ccgttaacca cggtatcgat atgcgaaccg
     7381 ctcagaacca cttcctgtgg atattcggtg ccattcaggc gaccgtataa attccccact
     7441 tcatcgaaac gtgtttccag cccgcttgct gccattcttt ttttaaactg ctgctgggtt
     7501 tccagccatt ccggcgaata aagtaaacgg gtcataccgc cggttgggtc agcgccaaaa
     7561 gaggaaagcc agggcagcgt ttcttctata gcttgacgga aatgtgtaat cataagaaag
     7621 tcctgtctca ataattattg cgcaaaggga tttttcgttt cgtatgacgt gttataaagc
     7681 gcgtcggaaa ttaaatactg gtaaatatca tcaacaattt cgatgccttc gacggcggct
     7741 ttgcgttgtt taatatcctg atcctgtccg ggataataaa cctgattaaa accgggcgcg
     7801 ggggtaatgg tatttaattc gcgcatggtc tggctaagat gttgacggaa taattcgctg
     7861 gaggagaaaa agttcgggtt aataactata tgtaattgcc ccaaattacg cccggcgtgt
     7921 aaatcgtcat acatcgaact aacctgtcgc ccgaacggta agccgagtaa gacgcctgag
     7981 aggacgtcaa tcatcatcat caggccatac cctttcggcc cagcggcggg gagcagagca
     8041 tgtaccgcga acggatcggt tgttggtgca ccgtttttat cgactgccca ggtatccggg
     8101 atagacatat tgcgcgagcg ggcgtcgagc acttttcccc atgcctgtac ggtagtcgcc
     8161 atatcgaagg taaggatctc atcgccttct cccggcgcgg caaaggccag tgggttagtg
     8221 ccgtagtaaa tttccgcgcc gccaaacggc accaccattg gatcggactg gcacagcgaa
     8281 atgccaatta atccggcgcg ggctgcctgc tgcacaaaat atgagattgc gccgctgtga
     8341 cccatccggc tgataccgac caccgcaacg cctttttgct gggcggtttt gatggcatgt
     8401 tccataccca ttttcgctgc gacctgtccg gcggcattgt cggcatgtaa aattgccgag
     8461 cacggtccgg tttcctcaag acgaaactcc ggttcgcggt tggtgccgcc ttttgaaatg
     8521 cgttcggcgt agtattcaac gcgcaccgcg ccatgagagt ggatccctct ggcatcggcg
     8581 taaaccaata cttcagccac ggttgcagcg tgctcacgtt ttaacccagc ctggcagagt
     8641 ttattctcga ttagctggtg gagtgtttcc cgactgattt tcatctgtct tcctttttaa
     8701 cgacggtgtg aagcatgact gcaattaaca tacagggaaa atatctggat tatgtgatcc
     8761 agacaggcaa aaaaatatag ttagaattta tttgataatc ttcgtgcttt taacctggtt
     8821 tttaaaacaa ccatgcttat taaaaaataa cgcgtaatag tctggcggtt atttgaattc
     8881 ttttgttaat aatccacgtg tgatattcat caccttattt actcgttgtc atcgataccg
     8941 taatcgccac attaacactg ctcgtgcaat tgccatgggt gcaattttta aggagttgtt
     9001 atgatccacg cctttattaa aaaagggtgt tttcaggatt cggtcagttt aatgattatt
     9061 tcacgaaaac tcagcgaatc agaaaatgtt gatgatgttt ccgtaatgat gggtacgccc
     9121 gccaataaag cgttattaga tactacaggt ttctggcatg acgattttaa taacgccacg
     9181 ccgaacgata tttgcgtggc aattcgtagc gaagcggcgg atgcggggat cgcgcaggcg
     9241 gttatgcagc agcttgaaga ggcgctaaaa caactggcgc agggttcagg cagcagccag
     9301 gcgttgacgc aggtgcgtcg ctgggacagt gcctgtcaga aattacccga tgccaatctg
     9361 gcgctgattt cagtggctgg tgagtatgcg gcggagctgg cgaaccaggc gctggatcgc
     9421 aacctcaacg tgatgatgtt ttccgataac gtcacgctgg aagatgaaat ccaacttaaa
     9481 acccgcgcgc gggaaaaagg cttgctggtg atggggccag actgcggtac gtcgatgatt
     9541 gccgccacgc cgctggcttt tgctaacgtg atgccggaag gcaacattgg cgtcataggc
     9601 gcttccggta ccgggattca ggagctgtgc tcacagattg cgctggcagg ggagggaatt
     9661 actcacgcga ttggtcttgg cgggcgcgat ctcagccgtg aagtgggcgg catcagtgcg
     9721 ctaacggcgc tggaaatgct cagtgcagac gagaaaagcg aagtgctggc atttgtttca
     9781 aaaccacctg ccgaaactgt gcgtctgaaa attgttaatg ccatgaaagc aactggcaaa
     9841 ccgacggtgg cgctgttttt aggttatact ccggcggtgg cccgcgacga gaatgtctgg
     9901 tttgcctcct cgctggatga ggccgctcgt ctggcttgtc tgctttcacg cgtcactgcg
     9961 cgacgtaacg cgatagcgcc tgtcagcagc ggatttattt gcggtttgta taccggcggt
    10021 acgctggctg ctgaagcggc gggattactt gccggacacc ttggcgtgga agccgacgat
    10081 acccatcaac atggcatgat gctggacgcc gacggacatc agatcctcga cctgggcgat
    10141 gatttttaca ccgtcgggcg tccccatccg atgatcgacc cgaccttacg caacctgtta
    10201 attgccgatc tcggcgctaa accgcaagtg cgcgtgttgc tgcttgatgt cgtgattggc
    10261 ttcggtgcga ccgccgatcc tgccgcctcg ctggtgagcg cctggcaaaa agcctgtgcc
    10321 gcgcgttcag acaatcaacc actgtatgcc attgccacgg tgacaggcac tgaacgtgac
    10381 ccgcaatgcc gctcgcagca aatcgccacg ctggaagatg cggggatcgc ggtagtgagt
    10441 tcgctaccgg aagccacctt gctggctgcg gcgttaattc atccgctctc gcctgccaca
    10501 cagcaacaca caccgtcatt actggaaaac gtcgccgtga ttaacatcgg attacgcagc
    10561 tttgcgctgg agctacaaag cgccagcaaa ccggttgtgc attaccaatg gtcgccagtc
    10621 gccggcggca ataaaaaact ggctcgttta ttagaacgtt tgcaataagg ggttcccatg
    10681 tttacatcag tggcgcaagc caatgctgcg gttatcgaac aaattcgtcg cgctcgtcca
    10741 cactggctgg atgtgcaacc ggcttcttca cttatcagcg aactaaacaa gggcaaaaca
    10801 ctgcttcacg ccgggccgcc aatgcgctgg caggagatga ccggacccat gaaaggggca
    10861 tgtgtgggcg catgtctgtt tgaaggctgg gcgaaagatg aagcgcaggc actggcaatg
    10921 ctggagcagg gggaagtgaa cttcattcct tgtcaccatg tgaatgctgt cgggccaatg
    10981 ggcggcatta cttctgccag tatgccgatg ctggtggtcg agaacgtgac cgacggcaac
    11041 cgggcgtact gcaacctcaa cgaaggtatc ggcaaagtga tgcgttttgg cgcttacggc
    11101 gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt taatgccagt attaagtgcg
    11161 gcgctggggc gcatggagcg cggtatcgat ctcaccgcga tgatggcgca gggcattacc
    11221 atgggcgatg agttccatca acgcaatatt gcttcctctg cactgttaat gcgtgcgctg
    11281 gccccacaaa ttgctcgcct cgatcatgat aaacagtaca ttgcggaagt gatggatttc
    11341 ctcagcgtga ccgatcagtt cttcctcaac ctcgcgatgg cctactgcaa ggcggcgatg
    11401 gatgtaggcg cgatgatccg cgcaggcagc atcgttacgg ctatgacccg caacggcaat
    11461 atgttcggga ttcgggtaag cgggctgggt gaacgctggt ttactgcgcc tgtaaacact
    11521 ccgcaaggtc tgtttttcac cggcttctcg caggagcagg cgaacccgga tatgggcgat
    11581 agcgcgatta ccgaaacctt tggtatcgga ggtgcggcaa tgatcgccgc acctggcgta
    11641 acacgctttg tcggtgcggg tggcatggaa gcggccagag cggtatctga agagatggcg
    11701 gaaatttacc ttgaacgcaa tatgcagttg cagatccccg gctgggattt ccagggggcg
    11761 tgcctggggc tggacattcg tcgcgtggta gaaaccggca ttacgccact catcaatacc
    11821 ggtatcgccc ataaagaggc ggggatcggg cagattggcg caggcaccgt gcgggcaccg
    11881 ctggcgtgct ttgaacaggc gctggaagca ctggctgaaa gcatggggat tggttgagga
    11941 acgcacaatg acgatcatcc atcctctact tgccagccgt agcgcaccga attatcgcca
    12001 gtcctggcgg ttagcgggag tgtggcggcg ggcgattaac ctgatgacgg aaagcggcga
    12061 actgttaacg ctgcatcgtc aggggagtgg tttcggcccc ggcggatggg tgcttcgccg
    12121 cgcgcaattc gatgcgttat gcggtggatt atgcggcaat gaacgaccac aggttgtggc
    12181 acaagggatt cgtctcgggc gtttcacggt taaacagcca cagcgttatt gtttgctacg
    12241 tattacgccg cctgcgcatc ctcaaccgct tgccgttgca tggatgcaac gcgcggagga
    12301 aaccgggctt ttcgggccac tggcattggc gacaagcgat ccgctgcctg ctgaattacg
    12361 ccagtttcgt cactgttttc aggccgcgct caatggcgtt aagaccgact ggcggcactg
    12421 gctgggtaaa ggccccggat taacgccaag tcatgatgac acgctgagcg gaatgctgct
    12481 ggcggcctgg tattatgggg ctttagatgc gcgctccggt cgcccgtttt ttgcctgttc
    12541 cgacaatctt caactcgtta ccactgcggt gagcgtcagt tatttacgtt atgccgcgca
    12601 aggatatttc gcctcgccac tcctgcactt tgttcatgct ctgagttgcc cgaaacgtac
    12661 cgcagcggcg atcgattcgc tgctggcgct ggggcatacg tcaggggccg atacgctgct
    12721 ggggttctgg cttggccaac aattattaca agggaaacca tgaaaacact ggttgtggct
    12781 cttgggggca acgccttact ccagcgcggt gaggcgctga cggcaaaaaa tcaatatcgc
    12841 aatattgcca gtgctgtacc cgcgctggcg cgcctggccc gttcttatcg gctggcgatt
    12901 gttcacggca acggaccgca ggtggggctg ctggcgttac agaatctggc gtggaaagag
    12961 gtagaaccgt atccgctgga tgtactggtg gcggaaagcc aggggatgat tggctatatg
    13021 ctggcgcaga gcttgagcgc acagccgcag atgccgcctg tcacgacggt gctgacgcgc
    13081 attgaggttt cgcctgatga tccggcgttt ttacagccag agaaatttat tggtccggtt
    13141 tatcagccag aagaacaaga ggcactggaa gcggcttacg gctggcagat gaaacgtgat
    13201 ggtaaatatt tacgccgggt ggtggcgtct ccgcaaccgc gtaaaattct cgacagcgaa
    13261 gccatcgagt tgttgctcaa agaggggcat gtggtgattt gcagtggcgg cggcggtgtg
    13321 cctgtgacgg aagacggggc agggagtgaa gcggtgattg ataaagatct cgccgctgcg
    13381 ttgctcgccg agcagattaa tgccgatgga ctggtgatcc tcaccgatgc tgatgcggta
    13441 tatgaaaact ggggaacgcc gcagcaacgt gccattcgcc atgccacacc ggatgagtta
    13501 gcgccatttg ccaaagccga tggttcgatg gggccgaagg taacggcggt gagtggctat
    13561 gtcagaagcc gtggtaaacc cgcgtggatt ggggcgttat cgcggattga agagacgctg
    13621 gcgggcgaag cggggacttg tatttcgctg tagtgatggg caccaggcaa ttggcacatc
    13681 cagcctgatg cgatgctggc gcatcttatc aggcctacag gcggtgtcgg aacggtaggc
    13741 cggataaggc gtttacgccg catccggcaa tcggtacacc atgcctgatg cgatgctggc
    13801 gcatcttatc aggcctacag tcggtgttgg agcggtgggc cggataaggc gtttacgccg
    13861 catccggcaa tcggcacacc atgcctgatg cgatgctggc gcatcttatc aggcctacag
    13921 tcggtgttgg aacggtaggc ctgataaggc gttcacgccg catgcggcaa gaaaatgagc
    13981 atcaattaac tgaacttgct ctgcgcccac atcacgccgc tggcatactc cggcggcagc
    14041 agtgggatta aggcttccag cgtcgcggtc agacgcgatg tgtcgctgtc ggtcaaattc
    14101 aaatgcccca ctttacggcc cggacggact tctttgtcgt accagtgcag atgcacaagc
    14161 ggcagtttca gccagtcata attcagatcg ctaccaatca gattgatcat caccgacgga
    14221 ttattcacta ctggttgcgg taacggcaga tcggtaattg cccgcagatg tagctcaaac
    14281 tggctgatgc tggcaccgtt ttgcgtccag tgaccgctgt tatgcacacg cggagccagt
    14341 tcgttgatca gcagaccttg tggggtgaca aaacactcca ttgccatcac gcctacatag
    14401 ctcagctcct gcataatcgc cgacaacatc tcttcagctt gcgcctgcag ctgtgcgttg
    14461 gcctgcggga aagcgacgct ggtgcgcaaa atgccgtcct gatgcaggtt atgcgtcagc
    14521 ggataaaaca cggtgctgcc atcaaaaccg cgcgcgccaa ccagcgacac ttcaccagag
    14581 aagtttatgc cctgctcgac aatacattcg ccgtagcatt cagcaggtaa ctgttcggtt
    14641 tcatcggcgc gtaaacgcca ttgaccgcgt ccgtcatagc caccagtgcg gcgcttaaca
    14701 atcgccagct cacctaaacg atcaaacacc gcaggccact cgctgcgttc ggaaagtaac
    14761 tgccacggtg cggtcggcag gtggagctta tcgaaaagct gcttctgagt cagacggtcg
    14821 gcaataatcg ggaacacatc gcggttcaca aaggccggat ggcgcgccag ctcgcgggtt
    14881 aatgcagttt ccggccagcg ttcgatctca gcggtaatca cgctttgttg aaaaggcacc
    14941 gccgccggtt cagcgtccag cccgaccggc cagacagcaa tgcctaacgg ttcgcctgcc
    15001 tgacgcagca tacggcctaa ctgcccgtta ccgaggacgc aaacctgttt catgccgcac
    15061 ctcgcgggtc cgggttttcc agcacttcat cagtttgtgc tttgcgccat tcattcagac
    15121 gctggtgcag ttctttatca tgagtcgcaa gaatttgtgc tgccagtaac gccgcgtttg
    15181 ccgcgccagc tttaccaatc gccagcgtac ccaccggaat gccgcgcggc atttgtacga
    15241 tggagtagag gctatcgaca ccgctcagtg cggcgctctg tactggcacg cccagcaccg
    15301 gcaccagcgt tttggcggca atcatgccag gcaggtgcgc tgcgccgcct gcgcccgcaa
    15361 taatcacctg ataaccgttc tcttcggcgc tttcggcgaa gctgaacagt ttatcggggg
    15421 tgcggtgagc agaaaccact tcaacgtggt gcgggacatt caggatttcg aagatttcgg
    15481 cggcgaactg catggtagcc cagtcgcttt tggaccccat cacgatggcg acacgcgccg
    15541 gattattgcg ggaagacatg cgtcttaaaa ctcctgtggt gcacaactct cggctttaga
    15601 gggcacagag aatagcacgg aaagagtgca aggaaaacgg ttgcgtaggc ggaattaacc
    15661 gtcagttgtt gagtttttaa tttgttctgc gtgttgatta tgcgctctgg ctgaattggc
    15721 aaagaatgcc catatcaaca caatgctcca tggaaaaaag caaataccga caatcaatat
    15781 aaatctttct acaaaacgtt ggttatgggg gcgggtgagt actaaataaa tgagccagaa
    15841 atagactatc caggaaatga ccgccgcagc taccagtgac aatgcgccaa aatagaagcc
    15901 gacaagaccg aaccagctaa tacacagtgg cgctcctgcc gtaagggcga tgccagaaat
    15961 caaaaatccg ttgcagcgtt gcataataat attccctgtt gttatttcac tcagagatta
    16021 ttaccttgct gattttaaaa aagatataca ggttgcgtta gttcggtgct ttttcagaat
    16081 ctgtccattc gtggtcagaa aggaaaatgg attagctcaa cgtcatccgc cgtgactttc
    16141 accatcgaac cttctgtatg ccaggcaccc agtaccacgc gaaaagccgt ttgctgattg
    16201 gcgataagtt catgcaccgc cgggcgatgg gtatgcccgt ggatcagcca ttgcacttga
    16261 tgtttttcca tcgcactgac caccgcgttt tggttaacgt ccatgatcgc cagcgattta
    16321 ctgctgttgg cttctttact gttcgcgcgc attcgcgcgg caatgcgttt gcgcacaaac
    16381 aacggcaggg cgaggaatag cgtttgcagc cagggtttgt ggactttggc acgaaaagcc
    16441 tgataacccg cgtcatcggt gcacagcgtg tcgccatgca taatcaacac ccgacgacca
    16501 taaagttcga gcaccttttc ttccggcaat aacgtcatgc cgctttcacg ggcaaagcgt
    16561 ttgccgagca gaaaatcacg gttgccatga atgaaataac agggaacgcc ggaatcggat
    16621 accgctttga tcgctgcggc catctggtga tgaagtgggt tgggatcgtc gtcgccgatc
    16681 caggcttcaa acagatcgcc aagaatatac agcgcatcgg ccttgcgggc ctcccccgct
    16741 aaaaaacgca gaaaaccggc ggtgatcgcc ggttcttccg cgcagagatg aagatctgca
    16801 ataaagagtg tcgccacgat tactcgctaa cggtcacgct ttcaatgata acgtcttctt
    16861 ttggcacgtc ctggtgcata ccgctacgac cggttgccac acctttgatt ttgtctacca
    16921 cgtccatgcc ttcaaccact tcagcaaata cgcagtagcc ccaaccttgc aggctttcgc
    16981 cagagaagtt caggaagtcg ttatcaacca cgttgatgaa gaactgggca gttgcagagt
    17041 gcggtgcctg agtacgggcc attgccagcg taccacgggt atttttcaga ccgttgttgg
    17101 cttcgttttt gatcggttct ttggtggctt tttgtttcat gcccggttca aaaccgccgc
    17161 cctgaatcat aaagccgttg ataacacggt ggaaaatggt gttgttgtaa aaaccttcgc
    17221 ggcagtagtc caggaagttt ttaactgttt caggtgcttt atcgtcaaaa gttttgatga
    17281 caatatcgcc gtgattggtg tggaaagtaa ccatttttgc atcctgttcc gtttgattgg
    17341 tgcttcaacc cggttcgggt catatatagg gtggtgttat agcataaccg cacgatcgga
    17401 tcatcacgca atgtatgctg attcgcgcgg gaaatatggg tattatacgc aactcaatta
    17461 cccacacatg tctaaacgga atcttcgatg ctaaaaatct tcaatactct gacacgccaa
    17521 aaagaggaat ttaagcctat tcacgccggg gaagtcggca tgtacgtgtg tggaatcacc
    17581 gtttacgatc tctgtcatat cggtcacggg cgtacttttg ttgcttttga cgtggttgcg
    17641 cgctatctgc gtttcctcgg ctataagctg aagtatgtgc gcaacattac cgatatcgac
    17701 gacaaaatca tcaaacgcgc caatgaaaat ggcgaaagct ttgtggcgct ggtggatcgc
    17761 atgatcgccg aaatgcacaa agattttgat gctttgaaca ttctgcgccc ggatatggag
    17821 ccgcgcgcga cgcaccatat cgccgaaatt attgaactca ctgaacaact gatcgctaaa
    17881 ggtcacgctt atgtggcgga caacggcgac gtgatgttcg acgtcccgac cgatccgaca
    17941 tatggcgtgc tgtcgcgtca ggatctcgat cagctgcagg caggcgcgcg cgttgacgtg
    18001 gtcgacgaca aacgcaaccc gatggacttc gttctgtgga agatgtcgaa agagggcgaa
    18061 ccgagctggc cgtctccgtg gggcgcgggc cgtcctggct ggcacattga gtgttcggca
    18121 atgaactgca aacagttggg taaccacttt gatatccacg gcggcggttc tgacctgatg
    18181 ttcccgcacc acgagaacga aattgcccag tccacctgtg cgcacgatgg ccagtatgtg
    18241 aattactgga tgcactccgg tatggtgatg gttgaccgcg agaagatgtc taaatcgctg
    18301 ggtaacttct tcaccgtgcg tgacgtgctg aaatactacg atgcggaaac tgtgcgttac
    18361 ttcctgatgt cgggtcacta tcgcagccag ctgaactaca gcgaagagaa cctgaaacag
    18421 gcgcgtgcgg cgctggaacg tctctatact gccttgcgcg gcacagacaa aaccgttgcg
    18481 cctgccggtg gcgaagcgtt tgaagcgcgc tttattgaag caatggacga cgatttcaac
    18541 accccggaag cctattccgt gctgtttgat atggcgcgtg aagtaaaccg tctgaaagca
    18601 gaagatatgg cagcggcgaa tgcaatggcg tctcacctgc gtaaactttc tgccgtattg
    18661 ggcctgctgg agcaagaacc ggaagcgttc ctgcaaagcg gtgcgcaggc agacgacagc
    18721 gaagtggctg agattgaagc gttaattcaa cagcgtttgg atgcccgtaa agcgaaagac
    18781 tgggcggcgg cagatgcggc gcgtgaccgc cttaacgaga tggggatcgt gctggaagat
    18841 ggcccgcaag ggaccacctg gcgtcgtaag taattgcgct attgccggat gcgagttttc
    18901 gcatccggtt atcgtctgcg ccaccacaac attcccatca gtagcatccc cggcaaccac
    18961 acccacatca attcagaaat aatcacctga tgcccgtacg gcgtggtgta acgagacaat
    19021 gcaaacggcg cgactttaat cacctgccag ggagcgaaaa agcgttcatc tgaccacggc
    19081 cacagccagc caaccccttt accgccagtg gttaccgaat ccagcaagct gtgcgatagc
    19141 aacgagacgg ttaaaaacag ccagcagcga atcagcccag ccctgaacca tcgacgtcca
    19201 atcagcacac ataacagcgg gacaacaaac gcaaacacca gcgaatgggt aaactcgcga
    19261 tgaccaaaaa cattgccgta agcaacgcca aatttaaacg acaatacatc agcatcgggc
    19321 agcatcgcca ggatgattcc ggcaaataac agacgcggag ggatggcttt cgaacccaac
    19381 cctaaaccaa tgcatagggg aacggcggcg tgcgtaataa cggttggcat gatggtcgct
    19441 tcggcaaaat gtcgatgcta tcagcatgga tgaacggggc gtagagggca aaagtctgaa
    19501 aagagaaccg gcctgttgat acaggccggg aaagggatca ggcaacaacc tgtacgctgt
    19561 gacctgcaaa actcactgtc tgaccggcga cgattttgca gcgtttgcgc gtttcaaccg
    19621 caccgtcgac tttcacctgg ccttcggcaa tcgcgatttt cgcctgcgcg ccgctttcgc
    19681 tccagccttc cagtttcagc aagtcgcaca gctcaacgtg cggatgttta cctaaagaaa
    19741 atgtcgccat gttactcatc ctgtggatca tgatattcaa cgcacgcctg tagcgtgttt
    19801 tcaatcagcg tggcaaccgt catcgggcca acgccgcccg gaacaggcgt aatgtatgag
    19861 gcgcgtttag ccgcgtcttc aaacacgacg tcgcccacaa ctttgccatt ttccagacgg
    19921 ttgatgccaa catcaatcac aattgcgcct tctttgatcc agtcaccagg aataaagccc
    19981 ggcttgccga cggcaacgat cagtagatcg gcattttcta cgtggtgacg cagattttta
    20041 gtgaaacggt gagtcacggt ggtggtgcaa cctgccagca gcagttccat gctcattggg
    20101 cgaccgacga tattcgatgc gccaatcacc acggcgttaa ggccgaaggt atcaatgttg
    20161 taacgctcaa gcagcgtgac gataccgcgc ggggtacagg gacgcagacg tggcgcgcgc
    20221 tggcacagac gaccgacgtt gtaaggatgg aaaccgtcca cgtctttgtc cggatgaata
    20281 cgttccagca ctttgacgtt atcaataccc gccggtaacg gcagttgaac cagaatgcca
    20341 tcaatggtgt tgtcggcatt cagcgcatcg ataagctcca gcagctccgc ttcgctggtg
    20401 gtttccggga ggtcataaga gcgggagacg aacccgactt cttcacaagc cttgcgtttg
    20461 cttgcgacat aaatttgcga tgcagggttg ctacccacca gcacaacggc cagtcctggc
    20521 gcccgcagtc cggctgcaat acgcgcctga actttttgag caacttcaga gcgcacctgc
    20581 tgcgcaatcg ttttaccgtc aataatcttt gctgccatca gagagaggat tccatctgtt
    20641 acgtaggtcg aaggggatgc gtctattttg tcagaagcgt ggcgcgctgt cagttttcgt
    20701 tttctgaaaa gtaaaacctt ctgtacctgg ctgattcaca cctatcatga taaccgtcgt
    20761 tggtttgccg ccatagtgag ctacatcaga tgcggtcgta cacttgttca gcaactggtt
    20821 caaaagccat tgactcagca agggttgacc gtataattca cgcgattaca gcacaccgta
    20881 gtatcaatgc gcccttagct cagttggata gagcaacgac cttctaagtc gtgggccgca
    20941 ggttcgaatc ctgcagggcg cgccattaaa attcaatcag ttacgccttc tttatttctt
    21001 ctataattcc agagtgggac attatcacca aaaatgtcgt ctattttcct cgcgtgctca
    21061 gtcaaatgat taggcgcaag gtgagcatat ctacgaacca tttctctgga ctcccatccg
    21121 cccatttcct gaagcactga taatgggacg cctgacggaa tcaaccagct tgccctggta
    21181 tgtctgaggt catggaaacg gaaattttca attcctgcac gacgacaagc tgatagccat
    21241 gctgtcttgc tgtcaatgcg catcttcctg agcgcaggca ttgatgttcc atctgctcgc
    21301 ttagccgccc tggtatgtac acccacattt gtgatgcttg cctgtttgat cacgcaacac
    21361 tttagaggcg gtatcgttca gcgccacacc aatggcgcag tttgatttgc tatcttctgg
    21421 attcaccctg gcaacttgtc gctgcatgtc aatctgttgc cactccagat ttatgatgtt
    21481 cgactttctc agaacagtta ccagcgcaaa tttgacgaca gatttcagtg gtttggggct
    21541 ctcatcaatc aggcgttttt cctgattctt ttgtaagtct cttgtgccat tccacctcat
    21601 acaggtttgc ctgcaaacag tactgatatt gcactgccag atattccggc tggcttcgtg
    21661 gttgttttca acagtgatga ggcatcgtgg catctcgttg aagaccatcg gggtaaaacg
    21721 gtttatgacg tggcatcagg ggacgcgtta tttatttctg agctcggtcc gttaccggaa
    21781 aatgttacct ggttatcccc ggcaggggag tttcagaagt ggaacggcac atcatggata
    21841 aaggatacgg aggaagaaac ctcgttgctg gaagcctgta agatgtatcg ggtgttgctg
    21901 aaccgagttg atacatcaac tgcccctgat attgagtggc ctgtgaaccc tgtcagagag
    21961 taatcattgg gattatgccg cagcacgtcg tgcgcaagaa cgtgctgcgg ctggatgcta
    22021 ttttttccct gaagcggaaa acattactac agtaccttta a
//
LOCUS       DAZFRE010000082        21839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFRE010000082 DAZFRE010000000
VERSION     DAZFRE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.082"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..161)
                     /locus_tag="ACHKUI_003566"
     CDS             complement(<1..161)
                     /locus_tag="ACHKUI_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599697.1"
                     /translation="MNIYFRIVISLAIIACIYGLLVPFLISMKDTVAVISGFALTFLT
                     PPCIYTIYKG"
     gene            complement(222..614)
                     /locus_tag="ACHKUI_003567"
     CDS             complement(222..614)
                     /locus_tag="ACHKUI_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599698.1"
                     /translation="MIDNRTASAIDQALQKHDTPVGPLFFVTHHGRTKKCLTRKTAIR
                     YLAFFMTTRAFERSGFRQRHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIA
                     RKQYTEKWFRKYDTWSAGYYELMATKPF"
     gene            complement(611..829)
                     /locus_tag="ACHKUI_003568"
     CDS             complement(611..829)
                     /locus_tag="ACHKUI_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599699.1"
                     /translation="MTLTATRIPEWVHQQALLVLRRYRCRRIFPHRIQRNGYLSLKVN
                     RRWRLLSKDDGRNWEIMSHERYSGEIKK"
     gene            complement(801..1016)
                     /locus_tag="ACHKUI_003569"
     CDS             complement(801..1016)
                     /locus_tag="ACHKUI_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599700.1"
                     /translation="MANLSPVSVVHEKVQIVMTIENGQVTDVCKVRDGELIASMDTFI
                     WLAERAGYQITAPAQEETSDINSNANS"
     gene            complement(1016..1375)
                     /locus_tag="ACHKUI_003570"
     CDS             complement(1016..1375)
                     /locus_tag="ACHKUI_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599701.1"
                     /translation="MTHEPINTYRRRIAVAALHRIKRKTGGDLLIVDLLDGNITTIEI
                     TEQFINQLLLRFEDITRGELGRVEGETEFRTAYQNAIGINQHTEYQAETGKLIIDNLL
                     QEVIDYAKEKYISGGIN"
     gene            complement(1571..1777)
                     /locus_tag="ACHKUI_003571"
     CDS             complement(1571..1777)
                     /locus_tag="ACHKUI_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599702.1"
                     /translation="MTETTSQQDNYEGICLEPDSFAVNVYHLLHATQVLHMSSNHETK
                     TLGSEILNFACEYAKTAAEKELAQ"
     gene            2116..2343
                     /locus_tag="ACHKUI_003572"
     CDS             2116..2343
                     /locus_tag="ACHKUI_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599703.1"
                     /translation="MINAEQLEKEISELKKELIWHKVAISALIRQVVSPEDKVKFMKQ
                     FSSSSKEFFTDEVHPEAGFWIRQLFSQDKRK"
     gene            complement(2512..2928)
                     /locus_tag="ACHKUI_003573"
     CDS             complement(2512..2928)
                     /locus_tag="ACHKUI_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599704.1"
                     /translation="MINKATTLDCLEELKNLGSLITLIAKATPDATLSSDIESCAGLA
                     WDMTNSISRKLSSAMLLQNKNSAINNRLRTQREACGLTTAELARLLDLDEEIIIQWES
                     GEYEPTISMLIPLANILGCDPMWLLTGETAAKDICA"
     gene            complement(3032..3688)
                     /locus_tag="ACHKUI_003574"
     CDS             complement(3032..3688)
                     /locus_tag="ACHKUI_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6599705.1"
                     /translation="MKSLGERLINARQKAGLTQDALAKKAGVTRVAISKAEQGLTKSF
                     NGDTLFKVAAALQCSPQWLQNGDEKDKHWENNVKSCPQRDTAYSYPVINWVQAGLFAT
                     SGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
                     GCYVIARKKSTNEATFKKYISEMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
                     "
     gene            3800..4027
                     /locus_tag="ACHKUI_003575"
     CDS             3800..4027
                     /locus_tag="ACHKUI_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599706.1"
                     /translation="MTLYEILKIQFKTNAAIGRRFPKKGRPRGSQGVGKWKTRGVPED
                     VAILCHLDTSIPYTHPSLANTEDDKPTGDQQ"
     gene            4024..4731
                     /locus_tag="ACHKUI_003576"
     CDS             4024..4731
                     /locus_tag="ACHKUI_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6599707.1"
                     /translation="MNTAIFNGKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQYEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            4840..5706
                     /locus_tag="ACHKUI_003577"
     CDS             4840..5706
                     /locus_tag="ACHKUI_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6599708.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGIGVLKLITEHNRAHAVF
                     SCHEHCYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTM
                     TTLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETS
                     ISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNLMEKSLSGADA
                     SDMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVD
                     SRLNGSDKEVRP"
     gene            5703..5897
                     /locus_tag="ACHKUI_003578"
     CDS             5703..5897
                     /locus_tag="ACHKUI_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599709.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            5894..6118
                     /locus_tag="ACHKUI_003579"
     CDS             5894..6118
                     /locus_tag="ACHKUI_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599710.1"
                     /translation="MIRHIVNSLYHRYNRCPRVGQWFTTSNGLVLRVCLVNAESQKVV
                     CQVQGRTYTLSYPLVAFQSGKMFKRLGGVV"
     gene            6115..7041
                     /locus_tag="ACHKUI_003580"
     CDS             6115..7041
                     /locus_tag="ACHKUI_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599711.1"
                     /translation="MSSKILGNVWDACAAYGVKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLEQLARTERD
                     KVAELKQQRRLSVLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGIKPETMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDYWQQPA
                     AHGGGRQRQVDVLAGLGAMSDKFGKSSNKLTF"
     gene            7052..7951
                     /locus_tag="ACHKUI_003581"
     CDS             7052..7951
                     /locus_tag="ACHKUI_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6599712.1"
                     /translation="MMTIDQREKQTRLQARMDELRAEMDELRAEIAFAQKGEKPWPYR
                     SCLMREGRGYCEKHGKYRTHILVWIDRNGEDREKISCCPDCLIAEASDLTMELSSLKA
                     EELTDNAGIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSC
                     GTGKNHLAVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLD
                     LLIVDEVGVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRIT
                     DGGRNWLSFNWPSYRSRIGGVAA"
     gene            7948..9348
                     /locus_tag="ACHKUI_003582"
     CDS             7948..9348
                     /locus_tag="ACHKUI_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6599713.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQASISGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAYTCEAAEDALKDVQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTKHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            9345..9602
                     /locus_tag="ACHKUI_003583"
     CDS             9345..9602
                     /locus_tag="ACHKUI_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599714.1"
                     /translation="MNNRTITVSPEQLRRQAQEMLRCAEQMEKTSVKKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFEILVKEFTA"
     gene            9654..10643
                     /locus_tag="ACHKUI_003584"
     CDS             9654..10643
                     /locus_tag="ACHKUI_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6599715.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESRATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPTVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            10661..11041
                     /locus_tag="ACHKUI_003585"
     CDS             10661..11041
                     /locus_tag="ACHKUI_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6599716.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            11175..11471
                     /locus_tag="ACHKUI_003586"
                     /pseudo
     CDS             11175..11471
                     /locus_tag="ACHKUI_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            11512..11587
                     /locus_tag="ACHKUI_003587"
     tRNA            11512..11587
                     /locus_tag="ACHKUI_003587"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11545..11547,aa:Ile,seq:cat)
     gene            11595..11671
                     /locus_tag="ACHKUI_003588"
     tRNA            11595..11671
                     /locus_tag="ACHKUI_003588"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11629..11631,aa:Arg,seq:tcg)
     gene            11685..11761
                     /locus_tag="ACHKUI_003589"
     tRNA            11685..11761
                     /locus_tag="ACHKUI_003589"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11719..11721,aa:Arg,seq:tct)
     gene            complement(11865..12014)
                     /locus_tag="ACHKUI_003590"
                     /pseudo
     CDS             complement(11865..12014)
                     /locus_tag="ACHKUI_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12202..12417
                     /locus_tag="ACHKUI_003591"
     CDS             12202..12417
                     /locus_tag="ACHKUI_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6599717.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            12422..12772
                     /locus_tag="ACHKUI_003592"
     CDS             12422..12772
                     /locus_tag="ACHKUI_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6599718.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALGFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            12836..13369
                     /gene="rrrQ"
                     /locus_tag="ACHKUI_003593"
     CDS             12836..13369
                     /gene="rrrQ"
                     /locus_tag="ACHKUI_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6599719.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            13586..13771
                     /locus_tag="ACHKUI_003594"
     CDS             13586..13771
                     /locus_tag="ACHKUI_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6599720.1"
                     /translation="MRKLKMKLCALMLPLVVSACGSMPVAPQPCVKPPDPPAWIMQPA
                     PDWQTPLNGIISPSENG"
     gene            13989..14255
                     /locus_tag="ACHKUI_003595"
     CDS             13989..14255
                     /locus_tag="ACHKUI_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599721.1"
                     /translation="MNVENLSNAHYIYNEMKELQRQKGILESGAGLGVTIQSAYQYSA
                     FLEAIRPHAVAELNSRIEEKKSALVNLGASFSIYEHNKAGWKPA"
     gene            complement(14261..14800)
                     /locus_tag="ACHKUI_003596"
     CDS             complement(14261..14800)
                     /locus_tag="ACHKUI_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6599722.1"
                     /translation="MDIKDKINTILLCDIAIHLGIETDIDPQLVKYAVSSGNDWVIKA
                     EYSHLDVDEPSKEDRDFVTAVLNMYRGLSNAFRKLSDDEQKELVRDHHLKIHDGAIQL
                     PGFDGNNECDYFSIIEAYQKIDRFPEQKQPIANTHSRTEHLYNAMLDEFKKIDAVNRS
                     WDLSKEELASILSTAPRSF"
     gene            14939..15289
                     /locus_tag="ACHKUI_003597"
     CDS             14939..15289
                     /locus_tag="ACHKUI_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6599723.1"
                     /translation="MPALIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            15437..15919
                     /locus_tag="ACHKUI_003598"
     CDS             15437..15919
                     /locus_tag="ACHKUI_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6599724.1"
                     /translation="MSGPPKIPPRLHLIRGNPSKRPVKDLKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRSETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDNVDPLAELLKARD
                     "
     gene            15919..17676
                     /locus_tag="ACHKUI_003599"
     CDS             15919..17676
                     /locus_tag="ACHKUI_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO6599725.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEKRGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADSEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPQHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVALFTAMSRFLVNG
                     GGVNDFLSTLDPDEDLLIL"
     gene            17673..17834
                     /locus_tag="ACHKUI_003600"
     CDS             17673..17834
                     /locus_tag="ACHKUI_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599726.1"
                     /translation="MKQLITDMTGLIGFGLLTAGVYLYAGLPASLMLSGCLLLLYALV
                     VSMRRKHAS"
     gene            17824..19050
                     /locus_tag="ACHKUI_003601"
     CDS             17824..19050
                     /locus_tag="ACHKUI_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6599727.1"
                     /translation="MLLDALFRSEPLENPSVPVTGEAAETDNIFARDVYVSPETSMKL
                     AAVYACIYVISSSVAQMPLHVMRKTNEHVQPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWGRLKNMWQRAVTALRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKNDE"
     gene            19043..19642
                     /locus_tag="ACHKUI_003602"
     CDS             19043..19642
                     /locus_tag="ACHKUI_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6599728.1"
                     /translation="MNDRETRCYSGEVRAEQYDNAPTHILGYGSVFNSRSEPLWGFRE
                     IIKPGAFDDVLNDDVRGLFNHDPNFILGRSSAGTLSLSVDERGLRYDIVAPDTPTICD
                     LVLSPMLRGDINQSSFAFRVARDGESWYEDDEGIVIREITRISRLYDVSPVTYPAYQD
                     ADSGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            19657..20874
                     /locus_tag="ACHKUI_003603"
     CDS             19657..20874
                     /locus_tag="ACHKUI_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6599729.1"
                     /translation="MKLHEMKQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQSVTTDSQGGYTVPTQMRNKIIDTMKAYGGIASVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTAASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEEMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            20951..21268
                     /locus_tag="ACHKUI_003604"
     CDS             20951..21268
                     /locus_tag="ACHKUI_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6599730.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            21277..21615
                     /locus_tag="ACHKUI_003605"
     CDS             21277..21615
                     /locus_tag="ACHKUI_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6599731.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQATVRVWMRFRRDVTATSRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            21612..>21839
                     /locus_tag="ACHKUI_003606"
     CDS             21612..>21839
                     /locus_tag="ACHKUI_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6599732.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISG"
BASE COUNT         5543 a         4938 c         5828 g         5530 t
ORIGIN      
        1 cccttataaa tggtataaat acatggcggg gttagaaacg tcagtgcaaa gccagaaatc
       61 accgctaccg tatccttcat tgatatcaga aacgggacga gcaatccgta aatacatgcg
      121 ataattgcca gtgatataac tattctgaaa taaatgttca tggtcctcct gctgtattca
      181 gcttgcctta tttaattgcg tcatggttaa tttcgtttac gtcagaatgg ttttgttgcc
      241 atcagttcgt aatatccggc gctccatgtg tcatattttc tgaaccattt ttctgtatac
      301 tgtttcctgg cgatgagtct gcgcagtcgt ctgattgttc gctggtgtgc gcgggtgtac
      361 tctgtggttg attctccacg tttccatatt tcattcccgt tgaagataaa acgcttgtca
      421 ggatggcgtt gtcggaatcc tgaacgttca aaagcgcggg tggtcataaa gaatgccagg
      481 taacgaattg ccgtttttcg agtgaggcat ttttttgttc ttccgtgatg tgttacaaaa
      541 aataacgggc cgacgggtgt atcatgtttc tgtaatgcct ggtcaatggc gctggcggtg
      601 cggttgtcga tcatttcttt atttctcccg aataacgttc atgactcatt atttcccagt
      661 tccggccgtc gtctttcgat aacagccgcc agcgacggtt aaccttcaga ctgagatatc
      721 cgttgcgctg tatccgatgc gggaatatac gtcggcatct gtaccgccgc aggaccagca
      781 acgcctgctg gtggacccac tcaggaattc gcgttgctgt taatgtcact ggtttcctcc
      841 tgagcaggtg ctgttatctg ataccccgct ctttctgcca gccatatgaa tgtatccatg
      901 ctggcaatca gctctccatc gcggactttg cagacatctg ttacctggcc attttcaatt
      961 gtcataacga tctgcacttt ttcgtgcaca acagatacag gggataaatt agccatcagt
     1021 taattcctcc gctgatatat ttttctttcg cgtaatcaat aacctcttgt aaaaggttgt
     1081 ctataattaa ctttccggtt tcagcctggt attcagtatg ttgattaatc ccgatggcat
     1141 tctggtatgc agttcggaat tctgtttcac cctccactcg acccaattca ccacgggtaa
     1201 tatcttcaaa gcgcaacagc aactggttta taaactgttc tgttatttct atggtcgtaa
     1261 tgttcccatc cagaaggtca acaataagca gatcaccacc tgttttacgt tttattcgat
     1321 ggagtgctgc aacagctata cggcgacgat atgtattaat gggttcatgt gtcatttgtt
     1381 atttcccgta tgctttacgc agaaataagc aggcaatatg catgtaattt tcaccgtatt
     1441 gtgcaataag acaggcggtc ttgtgtgatg ccatattctt tataaaagtc ataataaatc
     1501 ctcctgtgga ttaagattgt aacaatcccc ggcgataaaa ccgcaataaa cgttcagagc
     1561 atatttgttg ttattgcgct aattcttttt cggcagcagt ttttgcatac tcacatgcaa
     1621 aattcagaat ttcgctgccg agtgttttcg tttcgtgatt actggacata tgtaatacct
     1681 gtgttgcatg caataaatga taaacattta ccgcaaatga atcaggctcc agacaaatgc
     1741 cttcgtaatt atcttgctgt gaggttgttt ctgtcattgc ttctgaagtg catgcgagcc
     1801 tgtttttgac aattctcttt cctctaatca ctatatcggc aacatctatt gcctttacaa
     1861 cctccgggag aagttccggg tttgtataat caaagtcatc aacatggaga acagttatgt
     1921 tttcgaactt tttcatggct tcctcagctg acttatatgt cctgctatat agcgagtctc
     1981 agaagtgttt tcatattgag actgtttccg caatgattga taatcagtta ccggatgctt
     2041 atccgtgtcc ggcgcacgac cacacgtagc cgcgtgttgg tccccatttt caattcagct
     2101 ctcaatggag gataaatgat taacgcagag caactcgaaa aagagatttc agaactcaaa
     2161 aaagaattaa tttggcacaa agttgctatc tccgcattaa ttcgtcaggt agtttcacct
     2221 gaagataaag taaagtttat gaagcagttc tcatcttcat caaaggagtt tttcactgac
     2281 gaggttcatc cagaggctgg attttggatc cgtcaattat tttcgcaaga taagcgtaaa
     2341 tagcgtcatc aatactcgcg tcgctcttga atttaagagc gatgctcttt atatcctctg
     2401 aaattattac cagatcacca tatgttatat ttttattatt gcgcgcagtg cttttttgta
     2461 tgaaatcaag caattcacca atgagacaaa tatggtaagc atcacagttt ttcatgcgca
     2521 gatatccttt gctgccgttt cgccagttaa cagccacatc ggatcgcagc caagaatatt
     2581 tgccagtggg ataagcatac tgatagttgg ttcatactct ccgctctccc actggatgat
     2641 aatttcttca tcgagatcga gcagcctggc gagttcggcg gttgttaagc cgcaggcttc
     2701 gcgttgggtg cgaagacggt tgttgattgc agaatttttg ttctgtaaaa gcattgctga
     2761 cgatagcttt ctggatatgc tatttgtcat atcccatgcc agtcctgcgc atgactctat
     2821 atcgctagag agcgtagcat caggtgttgc ttttgctatt agtgtaatga ggctgccgag
     2881 gtttttcagt tcttcgagac agtcaagagt tgtagcttta ttgatcatga gatgatacct
     2941 cagttacgaa ctttgtttta tggtaactaa ggtatcaagg tgtggcaagt gatttttgat
     3001 actttggttt ctttttgtgt tttgtgtctg gttagaaaat atcccacctg gcatcaacca
     3061 caacacctac tatttcgcaa tcattgtcca tttctatgat tggatattgt ggattaaggg
     3121 gctttagaaa cgcctttccc atttcagaaa tatatttttt gaatgttgct tcattggtag
     3181 atttttttct ggcgatgacg taacaccctg aaaaaacttc tttatctggg ttgacaagga
     3241 tcgacattcc ttcaggaaat gttattccta cgggcgaagt cattgagtct ccgtgcactt
     3301 ccagccagaa ccccctctca ccagcgtatt ttacagaatg cctccaatta tcctgatcat
     3361 acatgttgta gtcatcacca gaagttgcga ataatcctgc ctgaacccag ttaattacag
     3421 ggtaagagta tgctgtgtct ctctgtgggc agctcttaac attattttcc caatgcttat
     3481 ctttttcatc tccgttctga agccactgcg gtgaacactg cagtgcagct gcaactttaa
     3541 aaagggtgtc accgttgaaa ctttttgtaa ggccttgctc ggctttactg attgcaactc
     3601 tggtgacccc agctttttta gccaacgcat cttgtgttaa cccagctttt tgccgtgcgt
     3661 tgatgagacg ttcacctaaa gacttcattt ttcttctcct ctcatggctg ttgatactaa
     3721 agtaacagaa tttcttgata ctttgggttc ctgtggttaa catcgttgga taacaaagta
     3781 tctggtgtga gactaaagaa tgacccttta tgaaatatta aaaattcaat ttaaaaccaa
     3841 tgccgctatt ggtcgcaggt tcccaaagaa aggaaggcct cgtggcagtc aaggtgttgg
     3901 aaagtggaaa acgcgaggtg ttccggagga tgttgccatt ctttgtcatc tggatacgag
     3961 cattccatat acacacccaa gtctagcgaa tacagaagat gacaagccca caggagacca
     4021 acaatgaaca ccgcaatttt taacggcaaa gcatccatga ccagcgttga aattgcagag
     4081 ctggtgggta aacgtcatga caatgtgaaa cgcactattg aaacattagc caaaggtggc
     4141 gttgtccggt ctcctcaaat tgaggtttcc gaaagaatca ataacttagg ttttaaagtt
     4201 caatatgagc attacctgtt tgaaggagaa caaggtaagc gcgacagcat cattgtcgtc
     4261 gcacagctct gtcctgaatt cactgctcgc ctggtagatc gctggcgcga actggaagaa
     4321 cagatccgta agccaatgag cgaaatcgaa atggttgccg cgatggctct tgaagcagtt
     4381 cgtcagcaga aacggatcac tcaggtggaa gaaaaagtca gccacgttgc tgaaacagtc
     4441 gagcaaatta aaaagggcac tattcgtgag ggctatgccg gatatcgcca actgaaagca
     4501 aaaaccggtt tgtcagatga taaatgccgc aatctggtga acgcctatca gattcctaca
     4561 gacacccatg agttcatgac gccggacgga ttgttgtcac gtcgcgcaat tgttgctgtg
     4621 gaaccgttta tggctgcttt ttatcgggtt atggaggaag cagaaccgcg agggactcgc
     4681 tggtatcacc cgaaaatggg gttatttcag gttattggtt ggcagcggtg aaaaaaagcc
     4741 gggagtaacc cggctcactc aacatcaata acggggagct gtttcgcata aaacggctcc
     4801 gaaacatcca agaacagttc taaagatatc agcagctata tgatcatttc aagaccaaat
     4861 attgattctg caatttcggg acgttacact gtctccgcac cttataaagc gggtgccggg
     4921 attggcgtcc tgaaattgat tactgagcat aaccgcgctc atgcggtttt ttcgtgtcat
     4981 gagcattgct acgcccaaat tatggtgggg cgtacagggc cgacttcggt cgggccgggt
     5041 tcggtagtct ccggtaacgc caaccctgta cgtctcacca cctctgtgat tggcgtccca
     5101 tgtggtgagt tttctaaaaa actgactacc ggggctgtca ctatgactac tctcccaacc
     5161 ctctctcaac ctgaaattgc catcgttgat ggtcaggctg ttacatcttc tttggctgtt
     5221 gctgacttct tctctaaacg tcatgacgat gttctgaaaa agattcgcat tttggattgt
     5281 tctccagagt tttgtgcccg caattttgcg gagacatcga tttcggtaaa tcaaccgaac
     5341 ggcggtacac gcaagctccc ttgctatcaa atcacacgag acggttttgc atttcttgct
     5401 atgggtttca cgggtaaacg tgctgcccgg ttcaaagagg catacatcaa cgcctttaac
     5461 ctgatggaga agagtttatc aggtgccgat gcgtctgata tgtcagctgt cgcacgaaac
     5521 gccagaggcg tatacctgca tttgcgtgaa atccatcaaa tctggacaag ccagctttat
     5581 ccaatgctta aggccgttga atctccgctg gctagcaaac tgtacgaccg tgttggtgat
     5641 gctgtttttg gcgctgcact tgttgattct aggctgaatg gttctgacaa ggaggttcgc
     5701 ccatgattag ttacgaaatc atcatctcca ctacggaata cagaaacgat gtatcagttc
     5761 gcacggatgt atctgtctgg caccgtcgct ataaatccag aaaaacagcg gaactgaaag
     5821 cggcagagat gtgtgaaacc atctcaatga aaggtagccc ggttaaatac gtaactacgg
     5881 cggaggtgcg tccatgatcc gccacatcgt taattccctg tatcaccgat acaaccgttg
     5941 cccccgagtg gggcagtggt ttaccaccag caacggcctc gttctgcggg tttgcctggt
     6001 caatgcagaa agtcagaagg ttgtctgcca ggtgcagggg cgtacttata ccctgagtta
     6061 cccgctggtg gcgtttcagt ccggaaaaat gtttaagcgt ctgggaggtg tcgtatgagt
     6121 agcaagatcc tcggtaatgt ctgggatgca tgcgcagcat atggcgtcaa aggtgcaaaa
     6181 ctgatgatta tggcgcgcct ggctgattat tcgaatgatg acggggtgtg ctacccgggt
     6241 gttgaaacca tatgtcgaca gcttggattg ggagaaagta cagtcagaac ggcaatctcc
     6301 gaactggagg ctgatggctg gctgacgcgt cagtcacgcc gcaaaggtaa ccgtaatacg
     6361 tccaatcttt atcatctgaa tgctgatcgg cttgagcagc ttgccagaac tgagcgggat
     6421 aaggttgcag aactgaaaca gcagcgcaga ctttcagtat tacgtgaccc ttcagattct
     6481 gaaccttcaa aatctgaacc gtcagaatct gtatgttcag gcgtgtttga cccttcagat
     6541 tctggcaaaa atacgcgttt gacccttcag aatctgactc cagatccaca aggtttaaaa
     6601 catgaaccac cagtaaattc aaaacatgaa ccgcaagata ttggcgcatc cgctgacgcg
     6661 tctgcaccag cgcgttctgc cagacaggaa tattcaccgg aatttgaaca ggcctggcag
     6721 gaatatccca aacgtgctgg tggcaattcc aaatcagcag ccttcaaagc ctggaaagcc
     6781 cgtatcaggg agggaataaa accggagacc atgcttgatg gcgtgaagcg gtatgccgcc
     6841 tgggtacgtg ctacaggaaa taccggcaca cagttcgtga agcaggctgc gacgttcttt
     6901 ggacccgatc gtcacttcga agattactgg caacagccag ccgctcacgg aggtgggcga
     6961 cagcgacagg tcgatgtcct ggctggcctg ggagccatgt ctgacaaatt cggtaaatcc
     7021 agtaacaaat tgacattctg aggtgacagc gatgatgacg attgaccaac gtgagaaaca
     7081 aacaagacta caggcgcgaa tggatgagtt acgggcagaa atggatgagt tacgggcaga
     7141 gattgcattt gctcagaagg gcgaaaagcc atggccttat cgttcctgcc tgatgcgtga
     7201 aggtcgcgga tattgcgaaa aacacggtaa atatcgtacg catatactgg tgtggatcga
     7261 tcgtaatggc gaggacagag aaaaaatttc atgctgccct gactgcttga tcgctgaggc
     7321 cagtgatttg accatggaac tgtcgtccct caaggcggaa gaactgactg ataacgccgg
     7381 aattgctctg cgttttcggg actgcgagtt tgataattat ctggaggtta atcctgacgc
     7441 agccagaaat cttgcggcct gtcgccgcta tgcggagaac tggccagata tgctggagaa
     7501 cggtaccagt cttgttatga ccggcagttg cggtaccggg aaaaatcatc tggcggtatc
     7561 aatggcaaaa cacatcatcc gtaactatct ggccagtgtg gagatcaccg acgtgatgcg
     7621 ccttacccgg gctgtgaaaa actgctggcg gaatgacagt gaaaaaacag cggatgacgt
     7681 cattgagcat tatgcgtcac tggatttgct gattgtcgac gaagtcggcg ttcagtttgg
     7741 cagtgcggct gaaatggcca ttttgcagga aattatcaat gcccggtatg agggtatttt
     7801 gccaactatc ctgatcagca acctttcacc ggaagaattg tgggcgttca tcagtccccg
     7861 gattgccgac agaatcaccg atggcgggcg caactggttg tcgtttaact ggcccagcta
     7921 ccgttctcgt atcggaggtg ttgccgcatg accagccaga acaccccggc atggcgtaac
     7981 gatgacctgg aaggcgctgt catcggtgcg ttttttctgc gtggggccga tccggaagtg
     8041 atggatattc tggccacact tccggcggat gtattttttg tgcgtcagta ccgggatatt
     8101 tacgcgggga tttgcagaca ggctagcata tccggcgtca ttgaccccgt actgctgtgc
     8161 aatgagatgc cggaacttgc cccggtgatt accgacaccg gacgcaaaac ctgggtgaag
     8221 tcttcactgg agcactatgt cgcagcgttg cggcgcaatg ccgcactgcg cgatgcagaa
     8281 aaaacactga ctgaagcatt acagaattta cgtgatgcgt atacctgtga agcagccgag
     8341 gatgccctga aggatgtgca gaacatgatg gcctcactgt cgaccggaaa gggcgtcatt
     8401 cagccggttc acattgatga tgtccttccg gaagtggtcg accgtgttga atgccgcaat
     8461 cagggactgg agaaatccag ggcgctgatg accggtattg atgaactgga cgcaaaaacg
     8521 ggcggtatgg agcccggaga cctggtattc attgccgccc gtccttcgat ggggaaaacc
     8581 gaacttgcgc tggacatcat cgacaaggtg actgagcagg ggcatggcgt gcttctgttc
     8641 accatggaga tggcgaacat ccagattggt gaacgtatgg tgtctgctgc cggtggaatg
     8701 ccggtatccc gtcttaagtc tgttgcccgt tttgaagatg aagactgggc gcgtttctcg
     8761 cagggcgtgg gacgaatgac ggggcgtaat atctggatgg tggatcaggc aaacctgacc
     8821 attgatgaga tatgtgcaac cacgaagcac caccggatga aacacccgga aacggcgctg
     8881 gtggtggtcg attacctcgg tctgattaaa acccgcagca cggggcgtca cgaccttgct
     8941 gtgggggaaa tctcaaaggg acttaaaagc ctggcaaaat ccggcggttt cccgctgatt
     9001 gctctgagcc agctctcccg aggcgtggaa tccagaccca ataaacgtcc catgaactca
     9061 gacctgaaaa actccgggga aatagaggct gatgccgata tcattctgat gctttacagg
     9121 gatgaggtgt ataacccgga tacacaggcc agaggcatag cagaaatcaa catcacgaaa
     9181 cagcgtaatg gcacactcgg gaccatttac cggcgttttc ataacggaca ttttctgcct
     9241 gtggaccagg agagtgcccg ggttctttcc actcccatga cgccgggcaa tccgcgcaga
     9301 tacagcaata accgcatgtc gggcagtaaa acggagcgtt tattttgaac aacagaacaa
     9361 tcactgtttc accggaacaa cttcgtcggc aggcgcagga gatgcttcgt tgtgctgaac
     9421 agatggaaaa aacgagcgtg aaaaaagata cgctccgcaa gcagcttact ccggcgcttc
     9481 gtgatctgct gcaggcaaaa caccgcacac aaaaggcggt ggatgagctg gtggattgcg
     9541 tggcggaact ggaaggacag gtaagccagt ttgaaatact ggtgaaggag tttactgcgt
     9601 gatgactgag tttttttctc tgtatgcatt caatatcgtt tgctgaggtg accgtgagag
     9661 cactgctgac ccctgaaatt gccccgcgta tggggattgt attgttcagg cccggttcag
     9721 agctgatgcc cctgtttatg caggggcgtg tcctgctgga gcctgagccg gaacgttatt
     9781 catctttcgc cagcggtgcc gttccggcgg catcacaacc gctggcggat gatcctgccg
     9841 ttcgggccgt gttccgcaat gaggcagtga tccgtcgtgc tggtggcgtg gaatgtcttg
     9901 aaagctggtt acttcgtgaa aaaggctgcc agtggcctca ttccgactgg cacagcgaga
     9961 acatgaccac aatgcgacac gctccgggtg caatccgtct gtgctggcac tgcgataatc
    10021 agctgcgcga tcagttcacg gaacggctgg aatcaagggc aacggataac tgtgcccgct
    10081 gggtgttgtc tgttgtgcgt cgggatctcg gttttgatga cagtcacgtt gtgacaatgc
    10141 cggaactgtg ctggtggctg attcgtaatg atctggcgga tgccttaccg gaaagtgcag
    10201 cccgtaaggc actgagatta ccgaagcctg ttgtgccgac tgttacccgg gaaagtgacc
    10261 ttgtgccttc ggttcctgcc accagcatca tccgggataa ggcgaaaaag gtgctggcgc
    10321 tgaaagtgga tccggagtcg ccggagtctt ttatgttacg cccaaaacgt cgccgctggg
    10381 ttaatgaaaa gtacacgcgc tgggttaaga cacagccgtg tgcatgttgt ggaaagcccg
    10441 ctgatgatcc ccaccacctg ataggtcacg gtcagggtgg aatgggaaca aaagcgcatg
    10501 acctttttgt gttgcctttg tgcagaaaac accatgacga actgcatgcg gataccgtgg
    10561 catttgaaga gaagtatggc tcccaactgg agctgatatt tcgttttatc gatcgcgcgc
    10621 tggcgattgg tgtgctgtcc tgattttgtg gagaaagttg atgcgtgata ttcagatggt
    10681 tcttgaacgc tggggggcat gggctgcaag tggtaacacc ggggtggact attctccgat
    10741 cgctgccgga ttcaaaggac ttttaccatc tgccactaaa ccacgtccgg cctgctgcga
    10801 tgatgacgga cttatcattg aaaactgtct tgctcgtctg aagcagaaaa aacctgagga
    10861 gtattcgctt ctcattgctc attatttgtt gcgaatatca aaaagacaga tagccaggac
    10921 gagaaagaaa agcgaaaaag caatacgaat tgagatgcag atagccgaag ggtttattga
    10981 cggatgtttg tctgtgctgg gggtaagact ggagatggac gactggctgc taaaaaagta
    11041 aaaaatgatt agtgcggtcc gcaaaaagta tgtcagtatg ttaagagtgg ttacttcgcc
    11101 acacagctta aacccgccgc gagcgggttt ttttatggct gaacgttaaa tgcgctggtg
    11161 gttgtgaatg ccgactgcgg cggtattttg gcgtgacggc gcaggtgccg tataccgatg
    11221 tctggacgca taaaccggtg cagttctatc ccgggaaaca tccgtgcgaa aaaccggcag
    11281 aaatgctgca gcagataatc agcgctagca gtcgtccggg tgacctgatt gcagattttt
    11341 tcatggggtc gggttcgaca gtgaaagcgg cactggcgct cgggcgtcgt gcaattggcg
    11401 ttgagctgga gactgaacgt tttgagcaga cggttcggga agtacaggat ttagtcagcc
    11461 agaacggatg atattgaaga attaattacg cgtcgttatt atgcggctcc cggcccttta
    11521 gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa tccagcaagg
    11581 gccaccatca cataccgcca ttagctcatc aggatagagc gccagccttc gaagctggtt
    11641 gcgcggggtt cgagtcctcg atggcggtcc attatctgta ccctgcgttg ttagctcaac
    11701 cggacagagc aattgccttc taagcaatcg gtcactggtt cgaatccagt acaacgcgcc
    11761 agacttattt ttcccggctc gcttttgcgg gccttttttt aaatgtctca caattcagac
    11821 ggttgacagt tgtctgtttt gcggggagtt tgttaaaaga aactggcatg gtgaatcccc
    11881 ctgtgcggag gggcaatcag cgagtaggta tatgggataa tcgcggattc aggtgctggt
    11941 actgaattca ccgggaggca cccggcacca tgcagttgac tgaaaccatg tatactctca
    12001 ggccccacgc acatgtgttg gggccttttt acatgcaaaa aaaagcccgc atatggatac
    12061 gggcggcaag gaacaaaaaa cgtgaagtga tctattcagc cagtggataa taccctgacg
    12121 ttaccatatt gcgcaatcgc gcattctttc tttttcgctc cccttataac tacgccatcc
    12181 gttcgctgcg gaggtgaggc tatgaaatcc atggataaaa tttcaacggg cattgcctac
    12241 ggcacctccg caggcagtgc tggctactgg tttttacagt ggcttgatca ggtcagtccg
    12301 tcacagtggg ctgcgattgg tgtactgggg agtctggttc tgggcttcct gacatatctg
    12361 actaacctgt atttcaaaat cagagaggac cgtcgtaagg ctgcacgggg agagtaattc
    12421 aatgactcaa aactatgaac tgattgtgaa agggatccgc aattttgaga ataaagttac
    12481 ggtaacttta gcgttacggg acaaaaaacg ctttgacggt gaaatttttg acctggacat
    12541 ctcgctggac cgtgttgaag gtgccgcgct ggggttttat gaggcagcag ccagaaggag
    12601 catcagacag gtcttcctgg atgttgctgc cgggttatgt gaaggggatg agcagtcgcc
    12661 ggaaaagcgc cccgtaattt tagaggcgca gaatgtgtgg ataacctaca aaggaaagct
    12721 accaggaaga attactggtt ctctgaagac tcctccggaa tcacaacctt aagtcactga
    12781 ccggaacaga taaacctgtc cgtgggcaga aaccgataaa tcctgataaa tatccatgaa
    12841 cgcaaaaatc agatacggcc tgtcggctgc cgttctggca ctgattgccg tcggtgcgcc
    12901 cgcgcctgat attctcgacc agtttctgga tgaaaaagaa ggtaaccaca caacggcata
    12961 ccgcgatggt tccggcatct ggaccatctg tcggggtgcc acgatggtgg atggaaaacc
    13021 cgtttttccc ggtatgaaac tgtcgaagga aaaatgcgac caggtcaacg ccattgagcg
    13081 tgataaggcg ctggcatggg tggagcgcaa tattaaagta ccactgaccg aaccacaaaa
    13141 agcgggtatc gcgtcatttt gtccctataa cattggcccc ggtaagtgtt ttccatcgac
    13201 gttttataag cggctgaatg ctggtgatcg taagggcgca tgcgaggcga ttcgctggtg
    13261 gataaaagat ggtgggcgcg attgccgcat acgttcaaat aactgctatg gacaggttat
    13321 tcgtcgtgac caagaaagcg cattagcctg ttggggaata gatcagtgag cagagtcgcc
    13381 gcgattattt atgctctggt tatctgcatc atcgtctgcc tgtcgtgggc ggtcaatcat
    13441 taccgtgata acgccatcac ctacaaagaa cagcgtgata aaaaagtcag tgagctgaag
    13501 cagctgaccg ccaccatcgc tgacatgcag cagcgtcagc gtgatgttgc tgcgctcgat
    13561 gcaaagtact cgagagaatt agccaatgcg aaagctgaaa atgaaactct gcgcgctgat
    13621 gttgccgctg gtcgtaagcg cctgcgggtc aatgccagtt gctccgcagc cgtgcgtgaa
    13681 gccaccggac ccaccagcgt ggataatgca accagcccca gattggcaga caccgctgaa
    13741 cgggattatt tcaccctcag agaacggttg atgacgatgc agaagcaact ggaaggggca
    13801 cagctataca ttcgtgagca atgtctcaga taaaaaccgg ccaaggataa tccgctaaag
    13861 attcgccggt ggcgaaagag agccaaggtg tcaacctacg ctattactta tgataatgca
    13921 acagacgaag cgggacatat tggcgcataa caaatcagtg caggctaact gcaggaagaa
    13981 cttagggcgt gaacgtagaa aacctaagta acgcacatta catctataac gagatgaaag
    14041 agctacagcg acagaaaggt atactggaaa gtggtgcagg gcttggtgtg acaatccagt
    14101 ctgcctatca atatagtgct tttcttgagg ccatacgccc gcatgcagtt gctgaactta
    14161 acagccgtat tgaggaaaag aaatccgcgc tggttaattt gggtgcttcc ttctctatat
    14221 acgagcataa taaggcgggt tggaaacccg cctaaagcac ttagaaactg cgtggagctg
    14281 tggaaagaat ggatgccagt tcttccttcg ataaatccca gcttcgattt acagcgtcaa
    14341 ttttcttaaa ctcatcaagc attgcgttat agagatgttc tgtacgtgaa tgagtattgg
    14401 caatgggctg tttctgttcg gggaagcgat caattttctg atatgcctca atgatactga
    14461 agtaatcgca ttcattatta ccgtcgaaac ctgggagctg aattgcccca tcatgtattt
    14521 ttagatgatg gtcacggact aattcttttt gctcgtcatc actaagtttc ctgaaagcat
    14581 tggaaagtcc gcgatacata ttcaagacag cagtaacaaa atcacgatct tctttacttg
    14641 gttcgtcaac atccaaatgt gaatattcgg ccttgataac ccaatcatta ccagatgaca
    14701 cagcatattt aacaagctgt ggatcaatat cagtttcgat acctaggtgg atggcaatgt
    14761 cacataacaa aatggtattg attttatctt taatatccat gagttaaccc tctgaagtaa
    14821 aaagtaatat ctacacccgt tggctcatga agtctattga tctgtctcat atcagcagca
    14881 aatctattcc atggagatac tcaatgcagg tcacctttga tttatataag ggcgaaacat
    14941 gccagcacta atcccacgcg cctgccgtaa gcgtggatgt gcaggtacaa ccacagacag
    15001 ttctggttac tgcgataaac atcgtggcga aggatgggta cagcatcaac gcggactgag
    15061 ccgccaccag cgtggctatg gctcgaaatg ggatgccata cgtgcgcgca tactgaagcg
    15121 tgataatcat ctgtgtcaga actgcctgcg caatgggaga gccgttgaag ccagaactgt
    15181 ggaccacatc attccgaaag ctcatggtgg cacggatgca gacagtaacc tgcagagtct
    15241 gtgctggccc tgtcataaag caaaaacagc gcgcgaacgc atcaattgat aacagctccc
    15301 atctgcaggg gaggggcggg tcaaatctct gcaaccctgg ctgttcagta ccgccgcctg
    15361 acctttcctc gcatcgccgc aggttcgaaa actttttttt ggaatgtgat taaatgattg
    15421 ataggtaaaa ccgattatgt ctggaccccc gaaaatcccg ccacgcctgc atttgatacg
    15481 aggcaacccc tcaaagcggc cagttaaaga cctcaaaaaa accgctaaaa aggatgaaaa
    15541 aggtctccct aaaattccgc aacatttagg ggcgcagggg aagtactggt tcaggcgaat
    15601 ggcggaagag ctgaatgcgg aagggatcat ttctcagctt gatgcgcgtg cgcttgagct
    15661 actggtggaa gcctataccg aataccggca tcactgcgaa acactcgatg ttgaggggta
    15721 cacctaccgc tcggaaacgc agaacggtga tgtgctgatt aaggcacacc cggctgctgc
    15781 gatgaaagcg gatgcctgga agcggatccg ggcgatgctt gcagaatttg gtatgtcacc
    15841 ggcaagccgg gcaaaagtaa ataccgccgg accggataat gttgatccgc tggcagagct
    15901 tttaaaagcg agagactgat ggcaaaagtg gctgacggga tccgctacgc cgaacgtgtt
    15961 gttgcaggag aaattgttgc tggcgaattt gtccgcctgg cctgccagcg ttttcttgat
    16021 gatctgaagt acggcgaaaa gcgggggatt tatttcagtg aaccccgtgc gcagcacatc
    16081 ctgaatttct acaaatttgt gcctcatgta aaaggggcac tggcaggcca gcccattgag
    16141 ttgatggact ggcatgtatt tatcctcatt aatatttttg gttttgtcat tccgctggtc
    16201 aatgaagaaa ccggggaagt tgtcatgcgc agcgatggca gcggacgtcc ggtgatggtg
    16261 cgccggttcc ggacggcgta caacgaagtc gcccgtaaaa acgcaaaatc aacactgtca
    16321 tcgggtatcg gcctgtatat gacgggggca gatagtgaag gcggagctga ggtgtattca
    16381 gccgcaacca cgcgtgacca ggccagaatc gtgtttgaag acgccaaaaa tatggtcaga
    16441 aaagcccggt cgacactcgg gcggttgttt gatttcaaca agctggcgat ttaccaggag
    16501 cagagcgcat caaaatttga accgctttcc tcggatgcaa acaacctgga cggtctgaac
    16561 atccactgcg ccattattga tgagctgcat gcacataaaa cccgcgacgt gtgggacgtt
    16621 ctggaaacgg caaccggtgc ccgtctgcag tccctgttat ttggtatcac cacggctggc
    16681 tttaacaagg aagggatttg ttacgagcag cgtgattacg ccatcaaggt attgcgaggc
    16741 tataacagcg acgtggaggg cgctgtaaaa gacgactctt actttgcgat catttacacg
    16801 cttgatgagg gagatgatcc gtttgatgaa acggtctggc agaaagcgaa tcctggcctg
    16861 ggcatctgta aacgctggga tgatctgcgt cgtctggcga aaaaagcgaa ggagcaggtc
    16921 tctgcgcggg tgaatttttt taccaaacac atgaatgtgt gggtcactgc cgaatctgcc
    16981 tggatggata tgattaagtg ggagaagtgc gaatacattg ctccacaaca tgagctgaaa
    17041 acatatccca tgtgggtcgg cgtcgacctt gctcataaga ttgatatctg tgcggcggca
    17101 aaactctggc gaaccgataa cggacatgtt catgctgatt ttaaattctg gcttccggaa
    17161 ggacggctgg aacgatgctc gcggcagcag gcagaacttt accggaagtg ggcggagatg
    17221 gataagctca tcctgacgga tggtgatgtt atcgatcatg ctcagataaa aagtgactta
    17281 ctggaatgga ttggcggtga aaacctcagg gaactgggat ttgacccgtg gagcgcaatg
    17341 cagttcagcc tggcactggc tgaagaaggg ataccgctgg tggaggttcc gcagacggtc
    17401 cgcaatctgt cagaggccat gaaggaaacg gaatcactgg tttatgccgg gcgtttccat
    17461 cacagcaatc atccggtcat gaactggatg atgtctaacg ttactgtaaa accggacaaa
    17521 aacgacaaca tcttcccgaa taaatccacg ccggaagcca aaatcgacgg ccctgttgcg
    17581 ctttttacag ccatgagccg ctttctggta aatggagggg gcgtgaatga ctttctgtcc
    17641 acgcttgatc ctgatgagga cctgttaatt ctgtgaaaca gcttattact gatatgaccg
    17701 ggctgatcgg tttcggtttg ctcactgctg gcgtttatct gtatgcaggt ctgccagcgt
    17761 ctctgatgct gtcgggctgt ttgttgctgc tttatgcact ggtggtgtcc atgaggagaa
    17821 aacatgcttc ttgatgctct gtttcgcagt gagcctctgg aaaatccctc ggttccggta
    17881 accggagagg ccgctgagac ggataatatt tttgcccggg atgtgtatgt cagtccggaa
    17941 acatccatga agctggctgc tgtctatgcc tgtatttatg ttatttcatc cagtgtggct
    18001 cagatgcccc tgcatgtgat gcgaaaaacg aatgagcatg ttcagccggc acgcgatcat
    18061 ccgttgttct ggctcgttca tgatgaacct aatgcctggc agaccagcta taagtggcgg
    18121 gaactgaagc agcgtcatgt gctggggtgg ggcaatggtt atacgtgggt aaaacgtaat
    18181 cgtcgtggag aggttaccag ccttgaatgc tgtatgccat gggaaaccac gttacttaac
    18241 accggtgggc gtcatactta cggggtgtat aacgaagagg gtgcatttgc ggtaagtccg
    18301 gacgacatga tccatatcag ggcgctggga aacaatcaga aaatgggact gagcccgatc
    18361 atgcagcatg ctgaaaccat tggtatggga atgagtggcc agcagtatac cagcgccttt
    18421 tttaacggta atgcccgtcc tgccggtatt atttctgtga aaaatgaact gaacgaacaa
    18481 agctggggca ggcttaaaaa tatgtggcag cgggcggtga cagcgcttcg cagccaggaa
    18541 aataaaacca tgttgctgcc tgcgcaactg gattaccgcg ctctgacagt ttctccggtg
    18601 gatgctcaga tcattgatat gaccaagctg aaccggtcga tgattgccgg gatttttaat
    18661 gtcccggcgc acatgattaa tgacctggaa aaagccacat tttcgaatat tacgcagcag
    18721 gcgattcagt ttgttcgcta cacgatgatg ccctgggttg cgaactggga gcaggagctt
    18781 aaccgtcgcc tgtttacccg tacagaacgg gctgccgggt attacgttcg tttcaacctc
    18841 acggggttgc tccgtgggac cccacaggag cgtgcgcagt tctatcactt tgccattaca
    18901 gatggctgga tgagtcggaa tgaagccagg gcatttgagg atatgaaccc ggttgacggt
    18961 ctggatgaaa tgctggtcag cgtaaatgca gcaaatccgt tgaataactt taaagatacg
    19021 aaaggcaaag aggaaaagaa cgatgaatga ccgtgaaacg cgctgttaca gcggggaggt
    19081 gcgggcggaa caatatgata atgccccgac ccacattctg gggtatggct cggtatttaa
    19141 cagtcgttca gaacctctgt ggggatttcg tgaaatcatc aagccggggg cttttgatga
    19201 tgtactgaat gatgatgtac gtggcttgtt taatcatgat cctaatttca ttctcggacg
    19261 aagttctgcc ggcacgttgt cattgtcggt ggatgaacgc ggtttacgtt atgacattgt
    19321 tgcaccggat actccgacta tttgtgacct ggtgctgtct ccaatgttgc gtggtgacat
    19381 taatcagtcc tcgttcgcgt ttcgtgtcgc ccgtgacgga gagagctggt atgaagacga
    19441 cgaggggatt gttatccggg aaatcacgcg catttctcgt ctgtatgacg tcagcccggt
    19501 gacatatccg gcctatcagg acgcagactc tggtgtccgc tcaatgaaag cctggcagga
    19561 agcgcgggcg agtggtgcgc tgaagaaagc tgttaacgaa cgaatggcgc gtgagcgtct
    19621 tttgaccctt cttaatgcat aaggatacta ctgacgatga aacttcatga gatgaagcaa
    19681 aaacgaaaca ccattgcaaa ggatatgcgt gcactgcatg aaaaaattgg tgataacgca
    19741 tggactgatg agcaacgggc agagtggaac agggcgaaag ctgagctgga tgcgctggat
    19801 gagcaaatcg cccgtgaaga agagttgcgc cgtcaggatc aggcatatgt ggatgagtcc
    19861 gggccggaag agcgccagaa taatgaggcg gagaacggga aaaaggcggt ggaagagaag
    19921 cgcgctgcgg catttaaccg ttttctgcgt gccggatttg cagaactgaa tgctgaagag
    19981 cgtaatctga tgcgtgaact gcgggctcag agtgtaacaa cggattctca gggcggatat
    20041 acggtgccca cgcagatgcg taacaaaatc attgacacca tgaaggctta tggcgggatt
    20101 gccagtgtgg cgcaacttct gaccacatca accgggcagg atatcacctg gtcaacgtct
    20161 gatggcacga ctgaagaggg cgaactgctg gcggaaaata cagccgcaac ggaacaggat
    20221 gtgacgttcg ggaccgctat tctgggggct aaaaagctgt catcaaaaat aattcgtgtg
    20281 tccaatgagc tgctccagga cagtggggtg gatattgaat cttatctggc aaaccgtatt
    20341 gcccagcgta ttggtcgtgg agaggcaaaa tatctggttc aggggaccgg aacgggatca
    20401 ccgttacagc caaaagggct ggcagcgtcg gtgacgggaa ccatccagac tgcagcctct
    20461 gccgctttca cctggaaaga aatgaatgcc ctgaaacatg ccattgatcc ggcatatcgt
    20521 ggtgggccga aataccgctg ggcattcaat gatgccacat tgcagactat tgaagagatg
    20581 gaggatgggc agaaacgccc gttatggctg ccggatattg caggcggtac gccggctact
    20641 gtgctgggga tcccttatgt tattgatcag gctattgacg ggattggtac cggaaaaaaa
    20701 ttcattttcc tgggggattt caaccgcttt atcattcgcc gcgttactta tatggaactg
    20761 aaacgtctgg ttgagcgtta tgctgagttt gatcaggtgg catttctggc tttccatcgt
    20821 tttgactgtg tgctggaaga tgtggcagcc atcaaggcgc tcactggcaa ataaccactc
    20881 gttgttcagt tacagaccgc gccgacgcgg tttttttatg cccgcacagt gttgcgggca
    20941 ggagtttctg atggcagcaa tagtggaaaa actcagggcg cagtgccgta ttgatacaga
    21001 tgatgcaact gatgatgagt tactgatgct gtatttccgg gctgcctgcc gcaaggcaga
    21061 aaattttatc aaccgtaagc tttatgagga gacggtgccg gaaggtgatc ctgaaggggt
    21121 gcttatagct gatgatgttt tgctggcgct catgttgctg gtcgggcact ggtacgaaaa
    21181 ccgggaaaat tcctcagatg tcagcaaggc accagtcccg tttggttttt cttctctgct
    21241 ggagccttat cgttttattc ctttgtagga ggagacatgc aggcgggcag attacgtgat
    21301 cgcgtaatta ttctgaatgt caccaccgcc cgctctccgt cagggcatcc ggtggagacg
    21361 gtgacggagg gagctaccgt atgggcagaa gttaagggta tcagcgggag ggagataatc
    21421 tcaggcggag cagaaaccgc tcaggctacg gtcagagtct ggatgagatt ccggcgcgat
    21481 gtgacagcga cttcacgtct gaaagtgctg accggtgcat ttaaaggggc cattctgggt
    21541 atagaaggtc caccaatacc ggatgcacgc gctacccggc ttgaaatact ctgcagcctg
    21601 aaggggaatg tgtgatggat ttcagtcttg atttttccgg cctggcggat attgcacggg
    21661 atctggagac gctcagcagg gcagaaaaca ataaggttct gcgcgatgcc acccgtgccg
    21721 gtgctgaagt tatgcgggat gcagttgttg aacgtgcgcc ggagcgaacc gggaaactga
    21781 agaaaaatgt ggttgttctc actcagcgtt caaagcgtcg gggggaaatt atctcgggt
//
LOCUS       DAZFRE010000083        21417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFRE010000083 DAZFRE010000000
VERSION     DAZFRE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.083"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..368
                     /locus_tag="ACHKUI_003607"
     CDS             <1..368
                     /locus_tag="ACHKUI_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6599733.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHKUI_003608"
     CDS             complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHKUI_003608"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6599734.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHKUI_003609"
     CDS             complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHKUI_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6599735.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3318..4076)
                     /gene="aroD"
                     /locus_tag="ACHKUI_003610"
     CDS             complement(3318..4076)
                     /gene="aroD"
                     /locus_tag="ACHKUI_003610"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6599736.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4107..4973)
                     /gene="ydiB"
                     /locus_tag="ACHKUI_003611"
     CDS             complement(4107..4973)
                     /gene="ydiB"
                     /locus_tag="ACHKUI_003611"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6599737.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4985..6250)
                     /gene="ydiN"
                     /locus_tag="ACHKUI_003612"
     CDS             complement(4985..6250)
                     /gene="ydiN"
                     /locus_tag="ACHKUI_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599738.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6477..7691)
                     /gene="ydiM"
                     /locus_tag="ACHKUI_003613"
     CDS             complement(6477..7691)
                     /gene="ydiM"
                     /locus_tag="ACHKUI_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599739.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7791..8147)
                     /gene="ydiL"
                     /locus_tag="ACHKUI_003614"
     CDS             complement(7791..8147)
                     /gene="ydiL"
                     /locus_tag="ACHKUI_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6599740.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8283..8390)
                     /gene="rprA"
                     /locus_tag="ACHKUI_003615"
     ncRNA           complement(8283..8390)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKUI_003615"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8576..9688)
                     /gene="ydiK"
                     /locus_tag="ACHKUI_003616"
     CDS             complement(8576..9688)
                     /gene="ydiK"
                     /locus_tag="ACHKUI_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6599741.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9902..12958
                     /gene="ydiJ"
                     /locus_tag="ACHKUI_003617"
     CDS             9902..12958
                     /gene="ydiJ"
                     /locus_tag="ACHKUI_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6599742.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTIIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTGFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12955..13365
                     /gene="menI"
                     /locus_tag="ACHKUI_003618"
     CDS             12955..13365
                     /gene="menI"
                     /locus_tag="ACHKUI_003618"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6599743.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13465..13653
                     /gene="ydiH"
                     /locus_tag="ACHKUI_003619"
     CDS             13465..13653
                     /gene="ydiH"
                     /locus_tag="ACHKUI_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6599744.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14202..14570
                     /gene="sufA"
                     /locus_tag="ACHKUI_003620"
     CDS             14202..14570
                     /gene="sufA"
                     /locus_tag="ACHKUI_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6599745.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14579..16066
                     /gene="sufB"
                     /locus_tag="ACHKUI_003621"
     CDS             14579..16066
                     /gene="sufB"
                     /locus_tag="ACHKUI_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6599746.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16076..16822
                     /gene="sufC"
                     /locus_tag="ACHKUI_003622"
     CDS             16076..16822
                     /gene="sufC"
                     /locus_tag="ACHKUI_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6599747.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16797..18068
                     /gene="sufD"
                     /locus_tag="ACHKUI_003623"
     CDS             16797..18068
                     /gene="sufD"
                     /locus_tag="ACHKUI_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6599748.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18065..19285
                     /gene="sufS"
                     /locus_tag="ACHKUI_003624"
     CDS             18065..19285
                     /gene="sufS"
                     /locus_tag="ACHKUI_003624"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6599749.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPLDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19298..19714
                     /gene="sufE"
                     /locus_tag="ACHKUI_003625"
     CDS             19298..19714
                     /gene="sufE"
                     /locus_tag="ACHKUI_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6599750.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19863..20867
                     /gene="ldtE"
                     /locus_tag="ACHKUI_003626"
     CDS             19863..20867
                     /gene="ldtE"
                     /locus_tag="ACHKUI_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6599751.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGEKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20931..21167)
                     /gene="lpp"
                     /locus_tag="ACHKUI_003627"
     CDS             complement(20931..21167)
                     /gene="lpp"
                     /locus_tag="ACHKUI_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6599752.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5591 a         5180 c         5389 g         5257 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata actatcgcga aaaaatcact ttagagcagt
       61 tatcgaaaaa atttcgtgcc agcgtcagtt atatctgcca tgaatttacc aaagagtatc
      121 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaacca gtaagtcttg
      361 ccggataaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgataatctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
      481 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcgcag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
      661 actttcaaca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgctggtt ggcatagcgg
      781 gcggcatctt cgaatgcgca ttcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gtggattgat tttaatgccg ggtttactgg agtcgaccca ccacagagta
     1021 aaggcctttt tcggatcttt cggttgcgga tcgcgcgcca acaccagcat atatgggtac
     1081 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
     1141 gtgtaagtgg tagtggcact gttgttgtct gagcctgcgc ctggctctgt caatgccagg
     1201 gcataggcgg gatcacccgt ttccagggtg ctctctgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taatcaaaaa tgccggagca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagtgc ccgcataaac
     1441 tcacgcgggt atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcattatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga aatccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggagcgatt tcgattaaat gcaggccgtc
     1741 ttcttttaac gtgaatacgg cgcgctcagt gatataacga acatccagcc ctcgctcgag
     1801 agcgattttt ccgctgaaag ttatttccgg tagttcccga ataaatttct tcacccgtcc
     1861 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
     1921 tgtgccgcag aaaacgattt tcttggaagt ggcactgata tcaataaatc caccggtgcc
     1981 catgatttta ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggcg cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taataccgcc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
     2221 caggccaatg ccgtcagcaa taccgacgcc gacattcccc actgcgcctt tgcgcatttc
     2281 gaataatgca cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaaga gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catacagttg
     2401 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgagcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgctatcgc
     2641 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgttccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     2881 tccccagtga ccgcataatg cccatttcac cagaccttct tgcgccagcg gactaatacc
     2941 acggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggtgtttg
     3001 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcagg tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa
     3241 atatttttat taaatacggt taaaaatact ttttttagtt cggcgggaga gggtattccc
     3301 gccgaaaata ttattgctta tgcctgatgt aaaatagtta ataccgtacg caaatcattt
     3361 accgagattt gccctggcgc agaggctttt tttaccgcac caaaagttgc cgccgaccca
     3421 aatacttcac cagccagacg agaaattacg ccagtttttg ccatcgacat cgtgatgatt
     3481 ggacgatcgg catactgctc ctgcatctcc agggtcgcgg caagcaacgt cagcacatcg
     3541 ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg
     3601 cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg gttggacatg
     3661 actactttca catcatgcgc gtgggcgtag gcgacggttt ctttaacctg atcatcgccg
     3721 gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc acgattgagt
     3781 gcaatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact gcggaaggta
     3841 aacagcagcg gtttttctgg catggtttca cggagaattt ttgctgccgc catgacagac
     3901 tccacattgg agaggtcggc atagtggtcc acacgccatt ccagaatatc aaagtccgct
     3961 tcacgatagg cgagagcttc ggatttcacg ctggcgatat ctttcgccat cagcgagacg
     4021 atgattttag gtgcgcccgt accaatgacg agatctttta cagttacggt tttcattttt
     4081 taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg tttaacatat
     4141 tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg ccacaacaac
     4201 atgccgtatc catcaatcgt tttgcaaccc gcttgttgcg cctgctgcaa taacttcgtc
     4261 atatgagggt tatacacgca ttcagtgacc agaagtcccg gatgtaacag actgatatca
     4321 ttaaccaatg attcattctc aaggggtttc atacccactt tggtgccatt ggttaaaatg
     4381 tcggcggaag ccagggcttc tgcaaaggct tgctgatcgg cgagatcggt gactgtcacg
     4441 acgcaatcgg tgttttcgtt aacccgctgc gcgaaggcga gagctttatc gaagaactca
     4501 tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg cgcgccaatt
     4561 gccgttgagg caccaccggc ccctaatagc accatcgttt tgcctttgat atcaaaaccg
     4621 ctctctttaa tggcgcgaat atgacccgtg ccgtcggtgt tatagccacg cagatagcca
     4681 tcatcattaa cgatggtgtt gatggctccc accagtttgg ccgctggcgt taattcatca
     4741 acatattcac acgccagttg tttgttcggc attgatacac cagttccgcg cattttgagg
     4801 gcttttaatc cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa ggccatatag
     4861 gtaaatggca atcccgcttt ttctaaggct ttattctgca tttcgggcga taaactgtgg
     4921 cggatgggat aggccatcaa cccaatcaat tcgtattttg cggtaacatc cataattaac
     4981 tcctttaacc tctatgctta attgtgttta accgggtgct aaatttatgc tcgccaaacc
     5041 gcacatcatt ttcaggaata atgaaaacgc ggtaatagcg gataaaaaca ataattgcgg
     5101 taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattgc aggccgatgt
     5161 tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc atcatcatat
     5221 atatactggt gactttggct ttgcttttgg ggaaaaactc tgacatgacc gaaacgccga
     5281 gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggctccggca ttacacacca
     5341 atggagaagg gtacaggtag ataatggcag ctgttattgt tgccagtgca gagttaaata
     5401 cattagccca gatgggacgg accatttttt tcagtagtgc ggcaaaaata aagacacaga
     5461 ccagcgaacc catactgtaa taagagatgg tttttaatgc ctcagcttct gacataccag
     5521 caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg
     5581 ctacaccgaa cagtactgat gaaacacctt ccagccagac taacggtttg ctgttcattt
     5641 gcggtaattc attagctacg ctggcgtcca ccaactggct ggggaatttg cttttcaaca
     5701 gcatcagcgt gatcagtaca aacagaatac ccggaataat cagcccatag ccgtaccaga
     5761 tgttattgag caacatatag ctcaccagca ttgggtagaa catttgtccg aatgacacca
     5821 tcgctttaac cagtatgacc gccgaaccag aggctttcgg aaagcattcc ataagcgcgg
     5881 ggtagccacc cgtatccagc gctgagttag cgatacctac gcacactgcc agacagtagg
     5941 cgagagttaa attcgggcaa gcaggaatac caaagaagaa tagcagatac attattactg
     6001 ccattaatat caccgcccga cgaccaaact tatcggagat cactccgaag aataaaatac
     6061 tgatcaatcg ccccaatccg ataccggaaa ttaaataggc aatgcccgcg ttgtcagtgg
     6121 aaaacttttc tgccaaagat gtcatgtttt gggcaagcgt aataacacta atgccgtgca
     6181 ggaaatagct gaagtaaata caaagaacag ccaggataaa tggcgtgctg aaagccttat
     6241 tttgagacat tttcacagct ccttgctgaa agataatttc gttaccgtac gtattgttct
     6301 ccgacggttt acataattac tatactgaag ataattttcc ctgggtacat ggcataaagt
     6361 ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt tccttagtta
     6421 gcgcagaaag agttatttat ccgtaagatc ttgcataacc tgaaaagaat attgcattac
     6481 ccacccggag cgatattttc ctttagcgtg tgatgccgcg tttctttgcg actgcgtaag
     6541 ccaataaaca gtgccagtat aaagccgata gccgcgatgg cggtatcgaa ccacataata
     6601 tcggcaatac ttctttgcga aagatgagcc gtaatcaacg gaatggtaaa tgttgcaatg
     6661 ctgcccgcac tgtaatagat ccccgttgct tttcctttag cgtagggaaa acgttccgcc
     6721 attaacgtca ggccaatttg tacaacaccg cctgcggagg taaaaccgat gacgaaagca
     6781 aatattatca ccacataaaa tgtgggatgc aggcagacgg tgagcagggc gataaatgag
     6841 ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tattacgaat gagtggagca
     6901 gtaataaata cacacagtag cgaacccacg gtgtagatac tgagtaattt gatcgacatg
     6961 gtgtatgaca tgccagcgac aaattgtccg tattgtgcca gccactggct aaccagataa
     7021 aacgttgcca ttgagatata gccatatagg gaataactgg ctaaatcgat aattgaacag
     7081 cgatgttccg tagagctggt ggttttcttt atgacaggta agtgacgacc cggatggggt
     7141 gggaacgtac aacggtataa aaacagagcg ttaataaaca taatgcctgc ggcaatcata
     7201 aaggaccaac cgaaccacag ttcagcccac actaacaggc tgataattag cggtaataaa
     7261 aactgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtacccgg tgagcgtgga
     7321 aaagcttcca tcaaactggg ataggtgcct gcatcgagaa aactgtttgc cattcccgcc
     7381 agaaagccaa aaacataagc gataatgatg ttattggtgt gcaggatgcc aaaaaagaag
     7441 gccatatagc agcacatccc gagcatgata aaagggcggc gaccaaagcg atcggataat
     7501 aatcctgcaa aaagcaagac acttaatcga ccaatgccca gtgatgagat aactatcgag
     7561 acgcccgcgg cattagtctg ccaaagcgtc tccagcgagg ccatattcag gctcatcaaa
     7621 atgacgccca taccatgcac caggtaatta aaatacaacc caagtgcggt agggtaatag
     7681 ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca
     7741 gtatccgttc attattgttg taacaacgac aattcctttc tgtggagaaa ttaacaaagc
     7801 cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt ccagtcaata
     7861 aaattgccat cagggtagac ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg
     7921 ttgttgccga tgcggttatt tattttctct atgatggcat gaagatgttt ttttcgttgc
     7981 tgacgcatag ccaacagttg ctcgactaca caatcaggaa tggcgcattt gccattttcc
     8041 cactgacgcc agctttcact gttaccggtc tgggcaatcc aggtcgcgca ttcatcaata
     8101 gtcatggcaa aaatatggcg aagtgcctgg agttcataag cgttcatcgt gtaatggctg
     8161 catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct tgaagagagt
     8221 cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga agcagaaaaa
     8281 tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa ttcgttgttt
     8341 cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt gagctaatag
     8401 taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga
     8461 atttgcgaca ggtactacca gctgaatagc ggtaagcagg aaaatatggg gaagagcaag
     8521 caaatatcaa ttagcagggg ttaacaatca ggcggcagag ccgcctgatg agaaattact
     8581 tattcgattt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg tccgtcggcg
     8641 gtgggacttc ttccacccac gcggcaaaca gacgccagga aacggctaac agaaccggac
     8701 caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacgcca gagaggatca
     8761 ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg ttatcgagcg
     8821 tgccaaccac accgctccaa actaacaata ccgttcccca ggtggtatcg ccagtccagt
     8881 agagccagat aatcgccgga atcagtaccg gcaacggtcc aagctggaca aggcaggaga
     8941 ggatcattaa caccgttagc aaagttgcat aaggtacgcc ggatacggcg aggccgatac
     9001 cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc gcgcggatag
     9061 cctgtgccgc cagcagcacg gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat
     9121 ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcgctg aagagaagca
     9181 tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg
     9241 tggtggtgcc aatataagga cggactttcg ccatgatcgc cgtccccccc atatccagca
     9301 aattgtgcca gcctgcatat agcttcgcgc caatcaccgg aatggtatta agccacgcca
     9361 gatcgggtaa cgtcatgtca ccactggaaa tggctttaat tagcgggccg ctgccgtcga
     9421 cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat aacagcgtca
     9481 tcaccagaac ggcgagagag cggcgaccaa acatgatctt ttgcaaacgt aacaataccg
     9541 gccaggtggc gataaccact gtaccggccc atgcaaagcc gagaataaag ggttgtacaa
     9601 tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga atttgtgcga
     9661 catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt gcgtcaaatc
     9721 gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt tcagcggttt
     9781 ttaacaggtc gattctgccc ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac
     9841 aactcaggca acacgcaaac gattaatctc tcatcacaga ccgcaggaaa gggtcaatat
     9901 aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca
     9961 agagctggag caacagggtt ttaccggcga tacggcgaca agttatgccg atcgtctgac
    10021 aatgtcgact gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc
    10081 aaccgcagat gtagcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgttgat
    10141 ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat
    10201 tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt
    10261 gcgcgttgag gccggggtga ttaaagacca actcaatcag tacctgaaac cctttggcta
    10321 cttttttgcg ccagaacttt cgaccagcaa ccgggcaacg ctcggcggga tgatcaatac
    10381 cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt
    10441 acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgaactggc
    10501 ggaaacgctg ggtaaatcca ataccataat cgggcgaatt tataacacgg tttatcaacg
    10561 ttgccgtcag caacgccagt taatcatcga caacttcccc aagcttaacc gctttcttac
    10621 cggttacgat ctgcgtcatg tctttaacga tgagatgacc gggttcgacc tgacgcgcat
    10681 tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcgactgg atattacgcg
    10741 cttgcctaaa gtacgccgtc tggtgaacgt caaatatgac tcttttgact ccgcgctgcg
    10801 taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt
    10861 gctcaacctt gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt
    10921 gcctgataaa gagatgctcg ggctgaacat tgtggaattt gccggtgatg atgaggcact
    10981 gattgacgag cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca
    11041 ggcaggagtg atcggctggc aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc
    11101 gatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgctgcta agccaattcc
    11161 gtttgctgag gatacctgcg taccgccgga acacctggcg gattatattg ctgaatttcg
    11221 cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt
    11281 cttgcacgtc cgtccagcac tggatatgtg cgatccgcaa caggagattt tgatgaagca
    11341 aatctctgat gatgtagtgg cgcttaccgc gaaatacggt ggtttgttgt ggggcgaaca
    11401 cggcaaaggt tttcgcgccg aatacagccc ggcgtttttt ggtgaggaac tttttgcaga
    11461 actgcgtaaa gtgaaagcgg cgtttgatcc gcataaccga ctcaaccccg ggaagatttg
    11521 cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt
    11581 cgatcgacag atccccattg cggtacgcca gcagtggcgt ggtgcaatgg agtgtaacgg
    11641 caacggttta tgcttcaact ttgatgctcg tagtccgatg tgtccgtcga tgaagatcac
    11701 ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtacgtgaat ggctgcgttt
    11761 gttggcggat cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt
    11821 cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata
    11881 tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc
    11941 gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta
    12001 tcacacccgt tatttacgcc ctctgcgcga ccaccttgtc gctacggtcg agagctacgc
    12061 accgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg
    12121 caaactctcg gaaaaacata ttggcatggt tgatttgccg ctgctgtcgg tcccctcgct
    12181 gcaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct
    12241 caatgcagag cagaaagcgc gcacggtgct ggtggtgcag gaccccttta ccagctatta
    12301 cgatgctcaa gtggtggcgg attttgtccg tctggtcgaa aaattaggtt tccagcccgt
    12361 gttactgcca ttttcgccaa atggaaaagc ccagcatatc aaaggttttc ttaatcgttt
    12421 tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc tgggtatgcc
    12481 aatggtaggc gtcgacccgg cgctggtact ttgttatcgc gatgaatata aactggctct
    12541 gggcgaggag cgtggcgagt ttaacgtctt actggcaaat gaatggctgg caagcgcact
    12601 tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac
    12661 cgaagttacc gccttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg
    12721 cgcgaaactg gaaaatgtca gcgtggggtg ctgcggcatg gcagggactt acggacatga
    12781 agcgaaaaac cataaaaatt cgctcgggat ctatgaatta tcctggcatc aggctatgca
    12841 gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagtc aggtaaaacg
    12901 ggttgaaggc acaggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata
    12961 tggaaacgaa aaatcaccct ggaagcactg aatgctatgg gggaaggaaa catggtggga
    13021 ttgctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta
    13081 gactcacgga caaagcagcc tttcgggttg ctgcatggag gtgcatccgt ggtactggcc
    13141 gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt
    13201 ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc
    13261 aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa
    13321 gggcgtttgt gctgttcgtc acgattgacg accgctattt tgtgacgttg tcaccagaaa
    13381 agtgtgacgc atattgcttt tggttattat ctgcacagta aacggtggta tactgtgcag
    13441 gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca actggccatt
    13501 gaatttttac gccgtgacca aagcaatctt tcacctgccc aatatctcaa gcgtctgaaa
    13561 caattagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa
    13621 atctacttcg cctggcgtct gggcgtgcat tgatcctgat ttaatgtcgc gagttcgcca
    13681 aatcggcctg gattaatctt cgtccaggtt cgagggcctt tttaatacag cctatctacc
    13741 cgcctgataa cgttcaaaaa taatacaaat cgcagtttgt gttaaaacag tgggttagct
    13801 ttatgagatt atccttatcg ccccttcaag agctaagcca ctgtgagtgc cggagataag
    13861 cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt
    13921 ctgaacgctg cactttttta cctatctctt aaatagttat tttcaataca actttttctt
    13981 tttcatcaca gccttaatgc tccttagata ataatcatta tctaacaatg agatacctaa
    14041 ttcttagcgt gcctgttaac ccacacatca gggtctatgc ttattaaaag caggaaaaaa
    14101 gcatgtttta cgctaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa
    14161 catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc gatggacatg cattcaggaa
    14221 cctttaatcc acaggatttc gcctggcagg gcttaacgct gacacccgca gccgctgtcc
    14281 acatccgcga gctggtggca aagcagccgg gtatgatcgg cgtgcgctta ggcgtgaagc
    14341 aaacaggctg cgcgggcttt ggctatgtgc tcgatagtgt tagcgagccg gacaaagacg
    14401 atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg atgcctttta
    14461 ttgatggcac ggaagtcgat ttcgttcgcg aaggacttaa tcagatattc aaatttcaca
    14521 accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag gcggtactat
    14581 gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta
    14641 taaagaagga ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga
    14701 ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct
    14761 caacgcctat cacgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga
    14821 caagctgaat tatcaggatt acagctacta ctcggcacca tcgtgcggta attgtgacga
    14881 caactgcgcg tctgaacccg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa
    14941 agaggtggag gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc
    15001 agtggatgcc attttcgact ctgtttcggt tgccactact tatcgcgaaa aactggcgga
    15061 gcagggaatt attttttgtt cctttggcga ggcgatccac gatcacccgg aactggtgcg
    15121 taaatatctc ggcaccgtgg tgccggggaa tgacaacttc tttgccgcac ttaatgcggc
    15181 ggtagcctct gacggtacgt ttatttatgt gcctaaaggc gtgcgttgcc cgatggaact
    15241 ttccacctat tttcgcatta acgcggaaaa aaccgggcag tttgagcgca ccattctggt
    15301 ggccgacgaa gacagctacg ttagctacat tgaaggctgt tccgctccgg tgcgtgacag
    15361 ctatcagtta cacgcggcgg tggtggaagt catcatccat aaaaacgccg aggtgaaata
    15421 ttccacggta caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt
    15481 caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac
    15541 cgggtcagcg attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg
    15601 tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat
    15661 gatccacatc ggtaaaaaca ccaaatcgac cattatatcg aaagggatct ctgccgggca
    15721 tagtcagaac agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa
    15781 tttcactcag tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta
    15841 tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg
    15901 tgaagatcaa ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat
    15961 gattgttaac ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga
    16021 agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt
    16081 aagtattaaa gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct
    16141 cgacgttcgt cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac
    16201 cttatcggca acgcttgccg ggcgagaaga ttatgaagtg acgagcggca cggttgagtt
    16261 taaaggcaaa gatttgcttg cgctgtcgcc ggaagatcgt gcaggtgaag gcatctttat
    16321 ggccttccag tatccggtgg aaattcccgg cgtcagtaac cagtttttcc tgcaaacggc
    16381 acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga
    16441 tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt
    16501 aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct
    16561 ggaaccggag ttatgcattc ttgatgagtc agactccggg ctggatattg acgcattaaa
    16621 agtggttgcc gatggcgtga actcgctgcg tgacggcaag cgttcattca tcattgttac
    16681 gcactaccaa cgcattctcg actacatcaa gcctgattac gtccatgtgc tgtatcaggg
    16741 acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg
    16801 ctggcttacc gaacagcagt aacgcactgc aacagtggca tcatttgttt gaagctgaag
    16861 gggcgaaacg ctccccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc
    16921 cgacacgtaa acatgaaaac tggaaatata cgccgctgga cgggctgacc aatagccagt
    16981 ttgtcagcat tgcgggggag atatccccac agcagcgtga tgccttagcg ttaacattag
    17041 acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc cgcactgagc gatgcaactg
    17101 aaggcagcgg atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc
    17161 aggcggaagt gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg
    17221 tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat gcatatcacc caaggcgtgg
    17281 caggtgaaga ggtgaatact gcccattacc ggcatcatct ggatctggcg gaaggtgccg
    17341 aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc tcgtcacttc accggcgcac
    17401 ggttcactat caacgtcgca gcgaacgccc atttgcagca tatcaagctg gcgtttgaaa
    17461 acccgctcag tcaccacttt gctcataacg atttgttgct ggctgatgat gccaccgcat
    17521 ttagccacag tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca
    17581 atggcgaaaa cagcacggtg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt
    17641 gtgatactcg tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca
    17701 aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc
    17761 acgccatcaa aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg
    17821 aagtggatac taaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg
    17881 cgacggtggg gcgtattgat gacgaacaga tgttttatct gcgctcgcgc gggatcaatc
    17941 agcaagatgc ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc
    18001 gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg tcaacgactg ccaggaggtg
    18061 caagatgact ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt ctcgtgaggt
    18121 aaacggtttg ccgctggctt atctcgacag tgccgccagt gcgcagaaac caagccaggt
    18181 gattgacgcc gaggctgagt tttatcgtca cggctacgcg gcggtgcatc gcggtattca
    18241 taccttaagc gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt
    18301 tattaatgcc cgttcggcgg aagaactggt gttcgtccgc ggcacgacgg aagggatcaa
    18361 tctggtcgcc aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag
    18421 tcagatggag caccacgcta acattgtccc ctggcagatg ctttgtgcac gcgttggcgc
    18481 agagctgcgt gtgatcccgc tcaaccccga cggtactctg caactggaga cgctgcctac
    18541 gctgtttgat gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttgggac
    18601 agaaaatccg ctggcggaga tgatcacact tgcgcatcaa catggcgcaa aagtgctggt
    18661 ggatggcgct caggcggtga tgcatcatcc gctggatgtt caggcgctgg attgcgattt
    18721 ttatgtgttc tccgggcata aactgtatgg ccctaccgga attggcattc tttatgtcaa
    18781 agaagccttg ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt
    18841 cagcctgagt gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc
    18901 caataccgga ggtatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct
    18961 taataacata gccgagtatg aacagaatct gatgcattac gcgctatcac agttggaatc
    19021 tgtaccggat ctcactctct atggcccaca aaacaggctt ggcgttattg cttttaatct
    19081 cggtaaacac cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg
    19141 taccggacat cactgcgcaa tgccattaat ggcctattac aacgtccctg cgatgtgtcg
    19201 ggcatcgctg gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca
    19261 acgtattcac cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga
    19321 aaaattgctg cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat
    19381 tgagttgggt cagcgtctgc cagaattacg tgacgaagac aaaagcccgc aaaatagcat
    19441 ccagggctgc cagagtcagg tgtggattgt gatgcgtcaa aatgcgcagg gaattattga
    19501 attacagggc gacagcgatg cggccattgt aaaagggctt attgcggtcg tctttattct
    19561 ttacgatcaa atgacgccgc aggatattgt caatttcgat gtgcgcccgt ggtttgaaaa
    19621 aatggcgctc acccaacatc tcaccccatc acgctcacaa ggtctggaag cgatgattcg
    19681 cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt
    19741 ggctcacatt gccgggtggt caatgtttac actggcctgc acaggagtgt tttggtttca
    19801 ggtgaacata agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta
    19861 caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg
    19921 ctgcctgggc ggttgattat ccgctaccac caaccggcag tcgactggtt gggcaaaatc
    19981 aaacgtatac ggtgcaagaa ggggaaaaaa accttcaggc catcgcccga cgttttgata
    20041 ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca aaacccggta
    20101 cgacgataac tattccttca caactattat tacctgatgc gccgcgtcag gggattattg
    20161 taaaccttgc agagctgcgc ctttattatt atccgccggg agaaaatatc gtgcaggttt
    20221 atccgatagg tattggtttg caggggctgg aaacgccggt gatggaaacg cgtgtggggc
    20281 agaaaatccc taacccaacc tggacgccta cggcgggcat tcgtcaacgt tcgctggagc
    20341 gtggcattaa attaccacca gtcgttcctg ccgggcccaa taacccacta ggacgttacg
    20401 cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca
    20461 gcgtcggttt gcgcgtcagt tcgggctgta ttcgcatgaa tgcaccggat attaaagctt
    20521 tattctccag cgtgcggacg ggaacaccgg tgaaagtgat caacgaaccg gtgaaatatt
    20581 ccgtggaacc taacgggatg cgttatgttg aagtacatcg accactatcg gcagaagaac
    20641 agcagaacgt tcagacaatg ccgtacacgc tgccagcagg ctttacgcaa tttaaagaca
    20701 ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg
    20761 ttgcggtgag cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc
    20821 agaatgggga accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaatagcag
    20881 gcaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg
    20941 tatttagtag ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc
    21001 tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga
    21061 gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga
    21121 gtagaaccca ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc
    21181 tagattgagt taatctccat gtagcgttac aagtattaca caaacttttt tatgttgaga
    21241 atattttttt gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc
    21301 tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttagat
    21361 aaagtgagat tatatagtga aatccgattt gattttttat tgcttctggt tatcgat
//
LOCUS       DAZFRE010000084        21307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFRE010000084 DAZFRE010000000
VERSION     DAZFRE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.084"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(92..2539)
                     /gene="glgP"
                     /locus_tag="ACHKUI_003628"
     CDS             complement(92..2539)
                     /gene="glgP"
                     /locus_tag="ACHKUI_003628"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6599753.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKSLFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2558..3991)
                     /gene="glgA"
                     /locus_tag="ACHKUI_003629"
     CDS             complement(2558..3991)
                     /gene="glgA"
                     /locus_tag="ACHKUI_003629"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6599754.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3991..5286)
                     /gene="glgC"
                     /locus_tag="ACHKUI_003630"
     CDS             complement(3991..5286)
                     /gene="glgC"
                     /locus_tag="ACHKUI_003630"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6599755.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5304..7277)
                     /gene="glgX"
                     /locus_tag="ACHKUI_003631"
     CDS             complement(5304..7277)
                     /gene="glgX"
                     /locus_tag="ACHKUI_003631"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6599756.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIYG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQRGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7274..9460)
                     /gene="glgB"
                     /locus_tag="ACHKUI_003632"
     CDS             complement(7274..9460)
                     /gene="glgB"
                     /locus_tag="ACHKUI_003632"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6599757.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9733..10836)
                     /gene="asd"
                     /locus_tag="ACHKUI_003633"
     CDS             complement(9733..10836)
                     /gene="asd"
                     /locus_tag="ACHKUI_003633"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6599758.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11028..11621
                     /gene="yhgN"
                     /locus_tag="ACHKUI_003634"
     CDS             11028..11621
                     /gene="yhgN"
                     /locus_tag="ACHKUI_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6599759.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11667..12962)
                     /locus_tag="ACHKUI_003635"
     CDS             complement(11667..12962)
                     /locus_tag="ACHKUI_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO6599760.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(12869..14965)
                     /locus_tag="ACHKUI_003636"
     CDS             complement(12869..14965)
                     /locus_tag="ACHKUI_003636"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO6599761.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(15105..16445)
                     /gene="gntU"
                     /locus_tag="ACHKUI_003637"
     CDS             complement(15105..16445)
                     /gene="gntU"
                     /locus_tag="ACHKUI_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6599762.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(16449..16976)
                     /gene="gntK"
                     /locus_tag="ACHKUI_003638"
     CDS             complement(16449..16976)
                     /gene="gntK"
                     /locus_tag="ACHKUI_003638"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6599763.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLRAGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(17115..18110)
                     /gene="gntR"
                     /locus_tag="ACHKUI_003639"
     CDS             complement(17115..18110)
                     /gene="gntR"
                     /locus_tag="ACHKUI_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6599764.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(18203..18898)
                     /gene="yhhW"
                     /locus_tag="ACHKUI_003640"
     CDS             complement(18203..18898)
                     /gene="yhhW"
                     /locus_tag="ACHKUI_003640"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6599765.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(19021..20058)
                     /locus_tag="ACHKUI_003641"
     CDS             complement(19021..20058)
                     /locus_tag="ACHKUI_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6599766.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            20396..20884
                     /gene="yhhY"
                     /locus_tag="ACHKUI_003642"
     CDS             20396..20884
                     /gene="yhhY"
                     /locus_tag="ACHKUI_003642"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6599767.1"
                     /translation="MSEIVIRHAETRDHEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            21120..>21307
                     /locus_tag="ACHKUI_003643"
     CDS             21120..>21307
                     /locus_tag="ACHKUI_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO6599768.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTTESIGNRWQSGHEDEIFTFSL
                     LNSITNTGLGSQFHGITF"
BASE COUNT         5287 a         5751 c         5217 g         5052 t
ORIGIN      
        1 atcaaagtta ttgtttattg tgtatagaaa taccgtgagt tccactctgt attacttcag
       61 cggaacacac ggtatttagc cgtatttgaa cttacaatct caccggatca atatgccaga
      121 tatgatcggc gtactctttg atcgtacggt cagaagagaa gtagcccata ttggcaatgt
      181 tcagcatcgc ttttgcggtc cactcttcct gacgctcgta gagttcgtcg actttatcct
      241 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      301 gcgaatcgac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      361 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      421 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaagataaag atattgtcag
      481 caccgacatg atccagcatc tcgacattcg caccgtccag cgtaccgata gtcagcgcgc
      541 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccccgcc agcgaaatct
      601 gttcagatag atcggccgcc ggaatgatca actgcgccag gctaacactg tagttcggga
      661 tgaacacgac tttcagcttg tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      721 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      781 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      841 tgatcacgtg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgca
      901 catcgaacag cgatttcgga tttaccacca cattcagctg ctgggcgata tactccgcca
      961 gacgcttttt gttctccagc ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac
     1021 agtgttgttg cagatcatta agcaggctaa ggtcggtgcg ccagttgcgg cccaggtgtt
     1081 cgtccaacac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1141 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aatagcgatt
     1201 gcaccatcag gttagagtgc agctccgata caccgttaac tttgtggctc accacaaccg
     1261 ccagccaggc catacgcaca cgacgaccgt ttgattcatc aatgatcgac gcccgtccca
     1321 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1381 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcgact ggccaggttt
     1441 ccagcgcctc gctcattagc gtgtggttgg tgtaggagaa gacctgacaa cacacttcaa
     1501 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1561 acagcaccgg atgagtatcg ttgaggtgaa tcgcaatttt atccgccagg ttatcgtagg
     1621 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1681 attcctgacg cagtcgtagc tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc
     1741 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtcg ccctggttga
     1801 atttaccgag gttaatttcg ctactggctt gggcactcca caaacgcaac gtgttggtcg
     1861 cgtcggtgtc gtaacctggg attatctgat cgtaagctac tcccagaatc tcttcggttt
     1921 caatccagcg cgttttttta ccttcctgct gaatgcgacc gccaaaacgg actttatagc
     1981 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tcaggcgact
     2041 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2101 aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacggc
     2161 ccaggccacc gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta
     2221 atcccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2281 tggaaagcgt acgaccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2341 gcgacaactg ggcacggttt gagcgtaacc agcgctccac gagacgatcg cgcacagcaa
     2401 ataacgttgc gttcagccat tcatgtttat tggcgacgac cggatccttg ccaatcgtaa
     2461 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2521 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2581 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2641 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca
     2701 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2761 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2821 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2881 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2941 cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     3001 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagaccaggt aaggcttcca
     3061 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3121 tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
     3181 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3241 tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca
     3301 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttgggctga
     3361 ctgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3421 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3481 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc
     3541 cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat
     3601 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3661 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3721 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3781 acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca
     3841 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3901 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3961 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     4021 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4081 ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac
     4141 gcagcgacgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4201 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4261 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4321 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4381 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta
     4441 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4501 agagagcggg aacgggtgcg cataagccag accggcttcg gtgatcttgg gaatcaaatc
     4561 tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag
     4621 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4681 cattgacggc gggttagctg gtttttcaac gaattcgata attttatcgt tctcatcaac
     4741 cgccataacg ccaaacgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4801 acgtgcgcct ttttcgacgt ggtcgataag catacgcgag tagtcttgct tgtagatatg
     4861 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4921 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4981 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctggatgtg
     5041 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt
     5101 gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg
     5161 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5221 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5281 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5341 tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5401 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5461 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5521 ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc
     5581 tccatcaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5641 ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg
     5701 ttattgccac gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg
     5761 gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gccgccgttc aaccagatca
     5821 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5881 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5941 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     6001 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg ctcccagagg taaatcataa
     6061 tgcagccaga aacgacgggc tgcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6121 gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc aattaacttc
     6181 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacgaaac
     6241 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6301 tctacccaat aacgcaggca ggcgctggca taatctacca ccgccggatg actcaaattg
     6361 agcgtgttac cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6421 ctacggttgt cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6481 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6541 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6601 atcgccaccg ggttgtaccc ccagtagtta ctaagcccca tgcgttgcag acgtggttcg
     6661 ctggcaaatt gtgccaccgg cagcagttcc agcgcggtaa tgcccaactg tttcaaatag
     6721 ttgatcatca ccggatgccc cagggcttta taagtgccac ggatctcgac cggaatctcc
     6781 gggtgcaggt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6841 gtgcgcggcg gagcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6901 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6961 tctttaaact ccccgtaaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     7021 aagcgatgcc cctcggcagg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7081 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag
     7141 cgatgttcct ggccattggc atcaaagaca cacagctcta cccgctcggc atgagcggag
     7201 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagt gggtttgcca
     7261 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccaga ggtggtagcg
     7321 tcaggctcag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt actgcgccgc
     7381 cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt
     7441 tacccggctg gttaataccg aagcgataat catgacgcgg caccggtgta aagttactgg
     7501 caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7561 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7621 tatggtggcg gtaggtgtgg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7681 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7741 gggcaaattc gttccccatg aacagtagtt tcttgcccgg gaatgcccac atccagccgt
     7801 agtaggcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg
     7861 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7921 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7981 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     8041 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctcc gccattgtca
     8101 ctgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8161 caagattctc gcgaccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8221 cgcgataaat cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8281 tccagtaaag tgcgttaccg acaaggaagt tactgacttc acggcgacca tagttgtaga
     8341 tcagcgtgtt ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg
     8401 tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggt acccagtcga
     8461 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8521 taccaaaacg gcgggtcggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8581 agggatgctc gttaatgggc agtagctcga gatgggtaaa gcccatccat ttagcataag
     8641 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8701 gccaggagcc aaggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8761 tgcgctcttc agtctgcaca accttttccg gcagcccgca aataagagac gcggtttccg
     8821 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8881 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8941 ttccgctctc tttacgcagt cgcatcggat gacggcgacc gtcccagtag ttgaattgcc
     9001 caaccaccga gacccgacgg gcgtttggag cccagacaga aaaacgcgta cctgtgacgc
     9061 catccatagt atctgcatgc gcgcctaagg tttcatacgg acgtaggtga gtaccttcag
     9121 ataatagcca ggcatccatt tcctggatta gcgggccaaa acggtaagga tcatcaatca
     9181 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9241 gaatgacacc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9301 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9361 gtcccgcggt ggttttatgc atccccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9421 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9481 gtgtcacccc atccaactga atttttgact tctggttgtg acattttttt gacctgaacg
     9541 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9601 cgtgtattcc gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9661 aaacatctgc gcttactcct gtattacgca ctagcagggg cggcatcgcg ccccagattt
     9721 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9781 cagcagctgg tcgcccacgg taaaggctga caggaactcc ggtcccatat tcagcttacg
     9841 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9901 agtgatttcc cgatcgttcg gaacaacttt cgcccacgga ttgtgcgcag ccagcagttc
     9961 ttccacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
    10021 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10081 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcaagctg
    10141 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ctaccggcag
    10201 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10261 gttcgcgagt tcatctgcca catggccata cagatggccc atttgggtta ataactcacg
    10321 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10381 aagatcattg gcgaataaac cacccagcga catcaacatc aggcttacgg tacagttacc
    10441 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10501 gtcaagaatg atgatggcgt catctttcat gcgcagagat gatgctgcgt caatccagta
    10561 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10621 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10681 gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac
    10741 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10801 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10861 gtgctgtata aatgtgccgg tctcctcctg gcacatcttt caccatacaa aaagcagcca
    10921 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10981 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    11041 tttctgcaac agttttattg atcctgatta tggatccgct cggaaaccta cctattttca
    11101 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11161 ttattgctct ccttgtgatg ctggtgttcc tgtttgcggg cgagaaaatt ctggcatttc
    11221 ttagcctgcg ggcagaaacc gtctccattt ctggcggcat cattctgttt ctgatcgcca
    11281 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11341 catttatcgt gccgttggca attccgctgg tcgccgggcc gactattctc gccacgctga
    11401 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11461 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11521 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11581 agatgttcct cgacggcatt cgaatgtgga tgaaggggta aggttcaaac aggtgttgca
    11641 cacaaccaga cgcggcacct gttttattac ctgaactgat aatgaccttc gcccaatcgg
    11701 ataacccttc cctcttcttc tgcgcggttc agccacacac taatttgttc gatggttaaa
    11761 ccagtacaag acgccagcct ttcaatactt atcgattcct ttgcaaggat cgccagttcc
    11821 gccacaaaga gttgataaaa atcgacaggg gtatgcgcgg gcattactgc gtaatttgcg
    11881 tcatcttcgt gcagcgaaaa cagttctggt tgagcgttac ggagtgctct gtcatacatc
    11941 agcccaggaa cgtctggcag tctgatcgtc acattctcag cctgttcagc actccatgac
    12001 gccccatttt ctactaatcg tgaattggct gattgcatat cctgatttgg tttaacctgc
    12061 ataggcagcc actgatgttt taatgcctcc atcgccccgg ttatcgttcc accggtcatt
    12121 cccgcacgta caaccattgc gctttcagca aggcaataaa tatatttatt ccgcgccatc
    12181 gcattgccga cggtgaaacg tacctctggg taaaacggcg aaatcaacac caggttgcct
    12241 gctataagcc cttcacgcca tttcactaac gtactcgttt ttaacaagct atcagctaat
    12301 acgccaacgg cagttccccc gacctccaga gccgaggcca ttgcacattc atcaatacct
    12361 cgcgcaccac cagagataac gcaaatcccc tgttgggcca gtttcgcggc cagttgttgg
    12421 gtatagcgca aatcgtcagt cggagcatct cgcgagccaa caatcgccat accttccgct
    12481 tgcagtaatg ctttattccc gcagccaaat aaaacgggag gtgcatccgt tcgcaatcgg
    12541 tttttcaagc gaacgggata atcggcatct ccgcgggtta agatccataa accggcgcgt
    12601 tgccacttat ctacagccaa cgccagacta tgaccacgcg ccagcaagcc aagaatacgc
    12661 tcctgagaaa tacgcggatc gctccagcct gtaagtttct cttgcggttg cggcaccaga
    12721 agctcggcgg gactgatacg ttggtgcttc agccataatg caaacttccc ccattcacta
    12781 ttactaagag gtttatactc actcgccgcc gcgcgagaaa aatcgctggt tagcaggaga
    12841 gttgcttgtg cattggctga aagattcatc aattttttac cgaggtagaa gcaagggcaa
    12901 gaggataaac cgtcgggcaa cctgcctggc gtaacagtgc tgtcagaact gtcagcgtcc
    12961 atgcagaatc cacgatatca tcaaccagca acgccggacc tggcatcaaa ggaggggtaa
    13021 tcacaaacgc cccgtcgaga ttttgacact ggtgaaaacg gttttgctgc attttctgcg
    13081 gtggattgtc cacgactttt tcaatggcat caataaaagg taagccaagt ttcgccgcca
    13141 gtcgccgggc aaaatcagga accaggtcga ggtgccttaa tgaaggaacg cagcataccc
    13201 aggttggttc aggatgcgga ttccagcgtt ggcgaaccat ttccgcacat gcctccacca
    13261 gttcatcact gaagcgcccc gtttttttct cctgggctac ctgctttccc cacccggaat
    13321 ctccccaacg agaaaggatt cgccccgtag atccttgtaa acccgcaggc aagttccctt
    13381 taaatccgta ttgcgtaaaa gccccagatg ccacctgttt attgagattt aatggcaggt
    13441 cagcgtgttt aataaataaa tttgccgcat ggagtaagcc gctatcaatg tcgggcgaca
    13501 acagcggatg ctgaagacaa ctgctacatt taccacaacg ttcggcactt ggctcatcga
    13561 gtgcacggcg cagaaattgc attttgcact ccttggtcgt gatatagctt tcaacgtctg
    13621 ccagttcact ttttctctga ttcatcaaat ggttaattcg ttcatgatca ggagaaaaac
    13681 tgacgatagt tctgcgccat aatttctcgg tatacaccac aggcgatgga ttttccgcta
    13741 ccaataattt cagtgctttt tctatttgcc cataacgaag attcgtccgt tgttcaatgc
    13801 ctcgtagggt aagaccgtca ttctcgctaa gtacgttgag tatttcatga atttgcgcct
    13861 ccgcaggaaa ggcgctttca cggaagaatt gatgaatagc gcggtcttca ccaccacaaa
    13921 gcaatatgcc aactgcggaa tctatagcac gcccggcacg ccccacctgt tggtagtaac
    13981 cgacaataga ccccggcatc tgataatgaa tgacgaaacc taaatcaggt ttatcaaacc
    14041 ccatacctaa tgccgtcgtc gcgaccagca ctttgatttt atttgccagc agtgcctgct
    14101 ccagatattc cctggcagta ttagaatctt ccgcgccttc acaagtcacg ccgctgtagt
    14161 aagcaaatgc actgatacca tttttcctta accactcggc gacaagttca gcatcgcgag
    14221 tagtcagggt ataaacaatt cccgatttgg aaaactgagg gataaccgtt gctaaccatg
    14281 ccagacggga tgactgttca cctaagacta aggcatcaag cgccaaactt tcgcgagcca
    14341 gcgttccacg ctgaatcaca atgtcaccca attgctgacg gatatcctca acgacacggt
    14401 tattcgctgt cgcggttgta cccagaactg gggtattcgc aggcagttgg cgcaaaatat
    14461 ctaatatacg tcggtaatcc gggcgaaaat cgtggcccca atcagagatg cagtgcgcct
    14521 cgtcgaccac aagcaagcca atacgatcgg ctataggata taaaaccgtt tcgataaagt
    14581 cctggtttgc caaacgttcc ggggaaacca gcaagcaatc gacctcacct tgcagcaact
    14641 tatcgctaat acgctgccat tcttccctgt ttgtagaatt caacgtttca gccgtaatac
    14701 ctaaccgttc agcggcggca acctggttac gcatcagggc aagcaaagga gagatgataa
    14761 ttgtcgggcc agcaccgcga tcgcggaaga ttttactggc gatgaagtac acggcacttt
    14821 tcccccagcc agtgcgttgg acaaccagca gttttcgtcg ttggttcacc accgcgtcta
    14881 tggcttccca ttgcccttcc ctgaaagtag cggtggtatt tcctaacatc cgctgtaaaa
    14941 gcaattcagc tccgtgcttt tccatcttta tctcctgcga tctttaggca gtgattagaa
    15001 cggattatta gcaggtaaat ttgcgagaga gatcaaaaag atgaagagag gagataagag
    15061 aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca gtaattaact caacagctgg
    15121 aacgcaatca tcccaacgat ggcaccaaca gtgccgagga tggtttccat catggtccag
    15181 gttttaagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaacccgcg
    15241 tcgttaacgt ggctgacaac aatcgaacca ccggcgatac aaatcgacag cgccgccatt
    15301 tgcgcaccgg agtagttcag ttgttcaatg accggcatta ccagtcccac cgccgttaaa
    15361 caggcaacag tggcagaacc ctgaatgatg cgcactgcag ctgccagcac gaagcaggtg
    15421 atggcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc gacaccagag
    15481 tcaaccagca cctgtttgaa cacgccgccc gcaccaatca ccagcagaat gatccccgcc
    15541 ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatacc ctgacgcatt
    15601 gccagaccgt aaatcgccac caggcaagca accagaatcg cggtaaacgg atgaccaata
    15661 aactcgaacc attcgtaagc ggtagagcct tccggcacaa agcgcgcggc aatggttttc
    15721 agccccacca gcaccagtgg caacaggatc agcgacaggc tgaatccgaa ggacggcatt
    15781 ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcagctc cacgtagcgg
    15841 ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg
    15901 ccaatcagga tcatccagcc aaagtcggcg ttcatctgcg atgccagcag cattggcgct
    15961 ggtccaggca ccaggaacgc agccgcggcc gccacccctg caaataatgg gattaccagc
    16021 ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca
    16081 atcgccactt caaagaacaa cggcagtgca cagaccagcc ccgcaaggcc gatggcataa
    16141 tgcgcgcggc tgtgaccgaa ggatttgagc attttgacgg caatctgatc gactgcgccg
    16201 gtttcatgta agatcttgcc aaacatggct cccagggcga caaccaccgc caggaagccg
    16261 agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa
    16321 aaaaggccag cccccataga caccaccatt aaagccagga aagcgtgcat acgcgccttc
    16381 atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta
    16441 gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc
    16501 ggttgatcga tatccaccac cagtacatcg gtttcgtccg cacccggctc ctgcagcgtt
    16561 tcaaactgcg tcactaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg
    16621 ctttcaatca catcaaaatc gcctttcagg tagatgaacg agagattcgg attacccgcg
    16681 cgtagcaagt cgcgatagtg ttttttcagt gcagaacaga cgatcagcga cactttattg
    16741 gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg
    16801 tcattcagcg gttcaccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg
    16861 ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc agatttgccg
    16921 ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg
    16981 gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattgtcc
    17041 ggccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt gtgagctact
    17101 tcaaatttgt gggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt
    17161 ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc aatactgccc
    17221 atccgctcac gcggcgtcag tacgctcgcc aaccgtggtt ccatcacctg accaatgtca
    17281 tgaccatgga aaccggcaat cgccatatcg tcaggaactt tcaaccccag acgctgacat
    17341 tcaaacgccg cgccgaccgc caggtcatca ttagtacaga acacgccatc cagctgcgga
    17401 tattcccgcc tcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca
    17461 accatcacgc tatatggcac caggcctgca tccagcatcg cctgttcgta tcccttctgt
    17521 ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg caatgtggcg atgcccgcga
    17581 gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc gacggcaata
    17641 tcgaggcacg gcgacttgct gtccatcagt tccaccactg gaatccccgc cacttcaatc
    17701 atctttaagg tgcgcggcgt gtgcgtacgt tcagtgagga tcaggccgtc gatattccag
    17761 gagagcatgg attcgaggcg ttcttgctcc atttccggtt tataaccgta gtgggccagc
    17821 atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcaaaa
    17881 acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc gttagagagg
    17941 atatcgggtg cgcgattggg aatataaccc agttcatcaa gagcggcggc aatcttgccg
    18001 cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt cattttggtc
    18061 acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag
    18121 gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt cccgattcct
    18181 caggaaggtt tgtgagataa atttagactg gcggtaaatc gaacagcagg atttcactgt
    18241 cgctatcagc atggatggaa atcgcctgct catcccagat tgccagacca tcgctggtcg
    18301 aggctttcac gccgttaatg gtgacattgc ctttcaccac ctggatccag acgcggcgtt
    18361 cagcggcaat ctgatgcacc gactgctcat ctttcaccag cgcccaacga tacagttcca
    18421 tatcctgatg cactttcaat gaaccatcac gcgcatccgg cgaaagcacc agttgtttgc
    18481 cctgcacggc atcgaagcga cgctgttcat aacgcggcgt aatgccgttt tcttcaggca
    18541 tgatccagat ctgatacaga tgcagacgct cggtgctgct tgggttgtac tctgagtgac
    18601 gaatacccgt acccgcactc ataatctgga actcgcccgc cggaacctgc tctttattgc
    18661 ccatgctgtc ctgatgctca acagtacctt ccagcacgta ggtcaaaatt tccatatctt
    18721 tatgcgggtg agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta atcacgcgca
    18781 gcgcagagaa gcccataaaa ttcggatcgt aatagttggc aaaagagaaa gtatgccagg
    18841 agtccagcca gccatgattt gcatgaccgc gttcatttgc tttgcgtaag tagatcattt
    18901 gtattcgccc cctgaatgat ttcgatgggc ttagtgtgga cccgatccgc ctgggatgat
    18961 agagggtgaa aattgacccc tctgttcaaa aaaattgaac tattcgaggg gccatcaaac
    19021 ttacctcgcg agagtcactg cggagggaga agcttgctca aaaccgcgtt caaggatttc
    19081 cagattcgta agaacttcag attccttgac gtaatttggc gcaccgttgg tgatggtttg
    19141 atacaacgca tcataaacgc gcccataatc gcccatctct ggcttcatct cttctcttac
    19201 cgtcacgccc tcgtcattga catactccag cacaccgacc gaatcatccg ctgcgaatcc
    19261 tggttcgccc ggcataatat tagccttcag gctggtttcc tgctggtcga taccgtattt
    19321 aataaacgaa cctttcttac cgtgaacgat aaatttcgga taatcgattt tcaccagatg
    19381 gctggttttg acgatggctt ttagatcgcc ataaaacagc tgcgcttcaa aggtgtcgtc
    19441 cggattggct ttattacgca ggctgcggat gtcataagcg acgtgatccg ggcgaccgaa
    19501 cagagaaata atctggtcca tcgtatgcac accaaggcca tagaacgcgc catcctgcgg
    19561 cagccccggt ttggtttctg ccaccgggcg gtaatagtca aaatggcttt ccacttcgac
    19621 aatctctccc agcttgccgc tttcaatcgc ttttttcgct gtcaggaagc aggaatcaaa
    19681 gcgacgattc tgatacggcg taacgatcag ccctttgctt ttcgccagtg caaacagctc
    19741 tttcgcctgc gcaattgtcg gagtgaacgg tttttcgacc agcacatttt tcccggcttc
    19801 cagcgcgcgc ttcgcgtact cgaagtggct gtccgcgtgg gtgcagacga caaccagctt
    19861 aacatcggga tcgtttagca cttcgtcgag atcgctggtg aaatggatat gggaataaat
    19921 gggggcctgt tcttccggct tcgcatggcg gcgaaaaata tgcgcgacat gccagctatc
    19981 cttgcggtta agtacatacg gcagatggta acgggtggtg cttttgccga agccaataaa
    20041 ggcgcagtta atgaccatga tttcgtcctt tttaaggtgg ttatttacac cttagcgcaa
    20101 agcggacgtg gttcctactg gagtgcgcat aacgaacaca agcacttccc gaggataaat
    20161 tgagaacgaa aggtcaaaaa aaaagccagc acccggctgg ctaagtaata ctggaagcaa
    20221 tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct tcctgatcgc gagacaataa
    20281 taatcattct cattcgcact tgtccaacac tttttgcaaa aaaatgcatt tgactcgcat
    20341 ttgaaagtca atgatgttga aagggacatt taccccaaag aggacaaagg aaggaatgag
    20401 tgagatagta atacgccacg cagaaacacg cgatcacgag gccatcaggc agattcacgc
    20461 ccagccggag gtgtattaca acacgctaca ggtgcctcat ccttcagaac aaatgtggct
    20521 ggaacgactc accgctcgcc ctggcatcaa gcagttagtc gcctgtattg atggaatcgt
    20581 cgtaggccat ctcaccattg acgtgcaaca acgcccacgc cgcagtcatg ttgccgattt
    20641 tggtatctgt gtcgactccc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    20701 gatgattgaa atgtgcgaca actggttgcg ggtagatcgt attgagctaa cggtgttcgt
    20761 cgataacgcg ccagcaatta aggtctataa aaaatttggc tttgagattg aagggactgg
    20821 caagaagtac gcgctgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    20881 gtaagatagt gccctttttc tgagatggaa aaagggtgtc attcaaaatc ggcatacctt
    20941 cctttaaggt atttattttc ccaataaata tatgagattt acctcataat tacttcctaa
    21001 agtgtaatat tttatttttt aatatatacg cctacaattt cctggagtaa ataaataaca
    21061 attaacaagc ataatattgc cattgataaa atagcatgcc ataaaaggac ttttcaggga
    21121 tgagtaatat tgtttatctg acagtaacgg gagaacaaca aggaagcatc tccgcaggtt
    21181 gtgggactac tgaatctata ggtaatcgct ggcagagcgg gcatgaggat gaaatattta
    21241 cattctcact cttaaatagt attactaata cggggcttgg ttcacagttc catggtataa
    21301 cattttg
//
LOCUS       DAZFRE010000085        20341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFRE010000085 DAZFRE010000000
VERSION     DAZFRE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.085"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            25..738
                     /gene="ybhM"
                     /locus_tag="ACHKUI_003644"
     CDS             25..738
                     /gene="ybhM"
                     /locus_tag="ACHKUI_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6599769.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVTTAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSSVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(774..1730)
                     /gene="ybhN"
                     /locus_tag="ACHKUI_003645"
     CDS             complement(774..1730)
                     /gene="ybhN"
                     /locus_tag="ACHKUI_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6599770.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1730..2971)
                     /gene="clsB"
                     /locus_tag="ACHKUI_003646"
     CDS             complement(1730..2971)
                     /gene="clsB"
                     /locus_tag="ACHKUI_003646"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6599771.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2968..3729)
                     /gene="ybhP"
                     /locus_tag="ACHKUI_003647"
     CDS             complement(2968..3729)
                     /gene="ybhP"
                     /locus_tag="ACHKUI_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6599772.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYLEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3862..4272
                     /gene="ybhQ"
                     /locus_tag="ACHKUI_003648"
     CDS             3862..4272
                     /gene="ybhQ"
                     /locus_tag="ACHKUI_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6599773.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4234..5340)
                     /gene="ybhR"
                     /locus_tag="ACHKUI_003649"
     CDS             complement(4234..5340)
                     /gene="ybhR"
                     /locus_tag="ACHKUI_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6599774.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5351..6484)
                     /gene="ybhS"
                     /locus_tag="ACHKUI_003650"
     CDS             complement(5351..6484)
                     /gene="ybhS"
                     /locus_tag="ACHKUI_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6599775.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6477..8213)
                     /gene="ybhF"
                     /locus_tag="ACHKUI_003651"
     CDS             complement(6477..8213)
                     /gene="ybhF"
                     /locus_tag="ACHKUI_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6599776.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     NSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            complement(8206..9201)
                     /gene="hlyD"
                     /locus_tag="ACHKUI_003652"
     CDS             complement(8206..9201)
                     /gene="hlyD"
                     /locus_tag="ACHKUI_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6599777.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(9204..9875)
                     /gene="cecR"
                     /locus_tag="ACHKUI_003653"
     CDS             complement(9204..9875)
                     /gene="cecR"
                     /locus_tag="ACHKUI_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6599778.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10104..11465
                     /gene="rhlE"
                     /locus_tag="ACHKUI_003654"
     CDS             10104..11465
                     /gene="rhlE"
                     /locus_tag="ACHKUI_003654"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6599779.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRTESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            complement(11698..11898)
                     /locus_tag="ACHKUI_003655"
                     /pseudo
     CDS             complement(11698..11898)
                     /locus_tag="ACHKUI_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            12002..14152
                     /gene="dinG"
                     /locus_tag="ACHKUI_003656"
     CDS             12002..14152
                     /gene="dinG"
                     /locus_tag="ACHKUI_003656"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6599780.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14180..15142
                     /gene="ybiB"
                     /locus_tag="ACHKUI_003657"
     CDS             14180..15142
                     /gene="ybiB"
                     /locus_tag="ACHKUI_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6599781.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            15283..16368
                     /gene="hcxB"
                     /locus_tag="ACHKUI_003658"
     CDS             15283..16368
                     /gene="hcxB"
                     /locus_tag="ACHKUI_003658"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6599782.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDEDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16434..16493
                     /gene="ybiE"
                     /locus_tag="ACHKUI_003659"
     CDS             16434..16493
                     /gene="ybiE"
                     /locus_tag="ACHKUI_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6599783.1"
                     /translation="MRLSANRQGNTCAERSVNA"
     gene            complement(16596..16856)
                     /gene="ybiJ"
                     /locus_tag="ACHKUI_003660"
     CDS             complement(16596..16856)
                     /gene="ybiJ"
                     /locus_tag="ACHKUI_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6599784.1"
                     /translation="MKTINIVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17121..17387)
                     /gene="ybiI"
                     /locus_tag="ACHKUI_003661"
     CDS             complement(17121..17387)
                     /gene="ybiI"
                     /locus_tag="ACHKUI_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6599785.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17461..18138)
                     /gene="ybiX"
                     /locus_tag="ACHKUI_003662"
     CDS             complement(17461..18138)
                     /gene="ybiX"
                     /locus_tag="ACHKUI_003662"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6599786.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18354..>20341)
                     /locus_tag="ACHKUI_003663"
     CDS             complement(18354..>20341)
                     /locus_tag="ACHKUI_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6599787.1"
                     /translation="GVGAFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRD
                     TFNTEQVEVIKGPSGTDYGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDV
                     NQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQH
                     NTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSITDTATMRFEHD
                     INDNATIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKIL
                     TNQTNLTSTFYTGAIGHDLSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLT
                     RNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITC
                     PAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQ
                     SGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQ
                     YGKKRVEGYEISVAGNITPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTL
                     WSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQL
                     NVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
BASE COUNT         4729 a         5385 c         5477 g         4750 t
ORIGIN      
        1 ttttttctaa ggacaactaa aagaatggaa tcatactcgc aaaatagcaa taaattagat
       61 tttcagcacg aagccaggat attaaacggc atatggctca ttaccgcttt gggtttggtg
      121 acaaccgcag gactggcctg gggcgctaag tatctcgaaa ttacggcaac aaaatatgat
      181 tcaccgcaaa tgtatgttgc tataggatta ttattacttt ctatgtatgg cttaagtaaa
      241 gatatcaaca agataaatac cgccatcgcg ggcgtaatat atttgttttt aatctctttg
      301 gtggcgattg tcgttgcgag tttagcgcct gtatctgcca ttatcgtcgt gttcagcact
      361 gcgggatcga tgtttttaat cagtatgctg gcaggtctat tgtttaaatt tgatcctggt
      421 tctcaccgtt ttatcattat gatgacgttg acagggttgg ccctggtaat catcgtgaat
      481 gccacattaa tgagtgaacg acccatttgg gtaataagtt gcttaatgat tgtcttatgg
      541 tcaggcatta tctcgcatgg acgaaataag ctccttgaac tgacaggaaa aagccatagt
      601 gaagaattat ggagttcggt tcgttgtgct tttataggtg cattaacact ctattactat
      661 tttatcggct tctttgggat tcttgccgct atagctataa cgcttgtctg gcaaaggcat
      721 acgcggtttt ttcattagcc gtaaatgcac atactaaaga acttaactat acttcacatc
      781 gccgcttcat ttttcgcccg tagcttcttc gcctggcttt ccagcaacag gtaacagatc
      841 agcgccagca gcagtgggat aaagtaatac agcacacggt aagcgagcag ggcggcgata
      901 attgtgcctt tggaggtatg ctccccagcc agtagcgcga taaacaccgc ttccagcaca
      961 ccaattcccg ccggaatatg cacgatgacg ccagcaatac tactaaccag caacacgccc
     1021 agtacaaaga aatagttcac gctttgacca agtaacagcc agataatcgc tcccattacc
     1081 atccagttaa cgctggaaat cagcatctgg gtgagggcga atttccacga aggcagcacc
     1141 agcttttgcc ctttgatggt catatggcgg tgcttcgcga aagcgcaaaa ccacaaataa
     1201 accgcgataa tcatcagtaa gccaataccg agaatacgca gcgtagtttg atcgacatac
     1261 cagtgatccg gcaactccac cacgcctgcg gtaaagataa cccccgctag caaaatatag
     1321 cctagccagt tggtggtaat actgagcgaa aaaatccgcg taatagtgct gcccggtaac
     1381 cccagtcggg agtacaaacg atagcgcata ccaatgccgc cgacccaggt actgagtgtc
     1441 aggttgaagg cgtagcagat aaacgacacc agcatcacct ggcgcttcgc cagtttgtga
     1501 ccgcagtaga aacgggcgag caggtcgtag caaccgtaaa tcagataact gacgaccacc
     1561 agcccgaccg cactaagcag cgcaacgcga ttgtagtcgc ggatgacctt ccagacctct
     1621 tcccagtcca cttttttggc gtagaccacc agcaacacaa tcaccgcgat aaaaaacagc
     1681 caggtgagga gtttctttgc taagcgccag cgcgggtgtg atttactcat cagggtttta
     1741 cccccgtgtt ttcagtttct acccgatcct gcgtttccat tgtcggttgt gcggggggac
     1801 caacctgcgc cagacgtggc gtgtgcgccg gaagccagcc caccagcgcc gggaagtggc
     1861 gtaaaaagtg gaacgccagc acgcttttgg tcaggttcca ccaggtgcgt ttcggcagca
     1921 tggtctcatc gacctgctga caatctgcgg cgataatgcc gttcagatta tcgcgcagcg
     1981 tctggttaaa attacgatcg tggatgatga catttgcttc gagattcagt gacaaactga
     2041 gcggatcgag attactggac cctactgtcg cccagtgatc gtccatcaat gccactttgc
     2101 cgtggagcgg gcggcggcgg tactcaaata cctgaacgcc gcctttaacc agatagttat
     2161 acagcaaacg tgcgccgact ctgacaatcg gcatatccgg ttcgccctga atgatcagtt
     2221 tgatccgcac cccgcgccgt gccgctttac gcaaggcgtg taaaaaacga tagccgggga
     2281 agaagtaggc gttggcgata atcacttccc gccgcgcctg agtgagcatt ttcagatagt
     2341 gacgttcgat atcatcgcga tgttcttcgt tatcgcgcca gaccagcaat acctgcgctt
     2401 ctcccggctg gcggttctct tccgctttat gatgacgtcg ccaccagcgt cgcgccgcgc
     2461 tctgtcctgg caggttttcc agctcaaact ggagaatatc ttcgacaatc ggcccttcga
     2521 ggcgaaccgc gtaatcctgt ttggcctccg ggccgtagct ggacatatgc tcggcggagt
     2581 aattcagccc gccaataaag gcgatacgcg cgtcgatcac cacaattttg cgatgcatcc
     2641 ggcgaaacac attggtgcgc ataccaaaaa ggcgagggcg gggatcgtag tagcggaaca
     2701 ccacgccagc tgccgtcagt tcattgacaa actcatcgct gagatccggc gaaccgtagc
     2761 catccagcaa gacttccgct tttaccccgc gttgcgctgc tgccaatagc gccgcatgca
     2821 gctgtttgcc gacgtcatcc tcaaaccaga taaacgtttc aagaatgatg cgttcttgcg
     2881 cctcgccaat cgccttaaac accgcgggat aatattgctc gccgttttcc agcaactgga
     2941 tcttattgcc ttcgcgccag ctacatttca taaatgaatc tccgcactta aaggggcatg
     3001 atcagaaagg tgtcgccatg tccgcagcgg caacgcggtt ggcgcgctgg cgctggcatt
     3061 tttgacgtag atcctgtcca gtcgtagtag aggaaattgc accggaaacg tgcgcgccgg
     3121 gcgtccgtgg gcgcgggtaa aaatctcatc cagcccggcc tgcactttta acggatgatt
     3181 agctttttgc cgccagtcgt tgaaatcacc cgctaccaat accggttcgc cgtccggtag
     3241 ctcattcacc cattcggcga gcatcgcaag ctgcgcctga cggtgcgcct cacgcaggcc
     3301 cagatgtacg cacatcacat gaatcgcttt cccggtcatc ggcggcacaa tgcggcagta
     3361 gagcacgccg cgcttttccg caccatcgac cgaaacatca cgattctcat aatgttcaat
     3421 gggataacgt gaaagaacgg cgttaccgtg atgtccttcc aggtatacgg cattgcgacc
     3481 gtaggcgaaa tcgctccaca tggtgtcggc gagaaactcg tagtgcgagg tatcgggcca
     3541 gttttccaca tgcagcggat gaacttcgtg cgcgcccatc acttcctgca ggcaaacaat
     3601 atcggcgctg acggtacgca cggcgtcgcg aagttccggc aaaatgaagc gtcggttaaa
     3661 cgcggtaaag cctttgtgaa tattgatggt gagcaccttg aacgaaaatt gttgtgtttg
     3721 atcgggcata attttcctgt ctttgcctct tatctcattg aaatagtgta gtcggcgtca
     3781 caaaaaggtg cggtcttacg gaattttccg taaagttcgg tactctgaat aagtagagat
     3841 aaattcttca ggagagaagc catgaagtgg caacaacgtg ttcgtgtcgc aactggccta
     3901 agttgctggc agattatgtt gcatttactg gtagtggcgc tgctggtggt gggctggatg
     3961 agtaaaactc tggttcacgt cggcgtagga ttatgcgcac tgtattgtgt cacggtagtg
     4021 atgatgctgg tgtttcagcg ccaccccgag caacgctggc gtgaggtggc agacgtgctg
     4081 gaagagctga ccacgacctg gtattttggt gcagcgctga ttgtgctgtg gctgttgtcc
     4141 cgcgtactgg aaaacaactt tttgctggca attgcagggc tggcaatcct tgccggccct
     4201 gcggtggtgt ctttgctggc gaaagataag aagttacatc accttacgtc taaacatcgc
     4261 gtacgccgct gaccctgtcg tggccgttat caccagtagc ggccacaaac tattccacac
     4321 aatatccaga ctcgcatcct tcaaataaat ctgcttggta atgtccgtaa agtggcgaat
     4381 agggttaatc cacgtcagat tttgcagcca cactggcatg ttttccaccg gcgaaacgta
     4441 accggaaagg agaatggcgg gcatcataaa gacaaatacg ccgataaatg cctgctgttg
     4501 cgttgaacag agcgatgaaa tcaacagacc gaatcccacc agcgataaac cgtaaatcac
     4561 catcgtaaag tagaacagcg ccagcgatcc ggcgaagggg atttgatacg cccagatacc
     4621 aatcgccagc acaatggtgg cctggaaggt ggcgacaatt aatgccggta cggctttgcc
     4681 gatgaatatc tgccaggtgg tgaggggcga aaccagcaac tgatcgagcg taccttgttc
     4741 acgttcacgg gcgacggaaa gtgaagtgac tatcattacg ccgatagtgg tgatcatggc
     4801 gatcagcgac ggcaccacaa accatttgta gtcgagattc gggttatacc agttgcgtac
     4861 caccagctcg ctgttgttgg gtttcggttt tccttccagc agctcctgct gataattttt
     4921 gacgatctgt tgcaggtagt tggcggcaat ttgcgcactg ttggagttac gcccgtcgag
     4981 gatcaactgc aacggcgcgg tctggaaggt atccagtttg cgcgagaagt cagccgggaa
     5041 acgcaccagt agtaacgcct tttgtgtgtc gatggtcggg cggatctcct gcgggctttt
     5101 cagcagcagc acatgggtaa aggcgctggc gcgggcaaaa cgttgagtaa gttccaccga
     5161 atgctcgccg ttatcttcat cgtagatggc gatggtggcg ttggtcactt ccagcgtggc
     5221 ggcgaacggg aacaggatca cctgaatcag cacgggtaaa atcagaattg cgcgggtttg
     5281 cggttcgcgc agcagcgact gcaactcttt gcggattaac gtccataagc gatgaaacat
     5341 gctcttctcc ctaatccagc cgacgtttgg ttttcagcca cgtcaggccg ataaacatca
     5401 ccgccgaagc gatcaaaaac agcacgttca ccaccagtac caccggaata ttcccggcga
     5461 ggaacaggct ttgcagggtg ctgacgaaat aacgtgcggg aataatgtac gtcaccgcgc
     5521 ggatcaccgc gggcatactg tctatctgaa atataaagcc ggaaagcata atcgacggca
     5581 gaaaagccgc gttcagggcc acctgagcgg cgttgaactg gttgcgggta atcgtggaaa
     5641 tcagcagccc catccccagg gtactgagta aaaacaggct ggagataaaa aacagaatca
     5701 gcagcgaccc gcgatacggc acgccgagaa taaacaccga caccagcata cacagcaaca
     5761 tcgccagcat cccgagaaag taataaggga tcagcttaca cagcagcagc tccgtgcggg
     5821 taatctccgt agagagcaaa gcctccatgg tgccgcgttc ccattctcgc gccaccacca
     5881 gcgaggtgag aatcgcgccg atgaccgtca tgataatggt caccgcaccg gggataataa
     5941 agtgctggct tatcgccgcc gggttaaacc agtagcgggt ttgtacatca ataagcggtt
     6001 caaaagtctg cccgttatcc tccgcgcgct gcatttgcca gatctgccag atcccttcga
     6061 catacccctg cacaaagtta gcggtattcg gctcactgcc gtcggtgatc acctgaatcg
     6121 gtgcggtggc gttggcgcgc tccatctgtt cggcgaaatc caccggaata accaccagac
     6181 cgcgaatttt ccccgcctgc attttggcga tcagttcctg acggttatcg ctgatggtgg
     6241 cgtcgatgta gggcgaaccg gtcatggtgt gggtgaaatc cagcgccgct tcgctacgct
     6301 gttccagtaa aatcccgacg cgcagcttgc tggagtcgag gttaatgccg tatccaaaaa
     6361 taaacagcag tagcagtggg atcactaccg caatcagcca gctactcgga tcgcgaacaa
     6421 tctgccgcgt ctctttgacg cacagcgccc gcacgcgacg ccaggacagg atcggattac
     6481 tcattgccat gctccttatc ccagtcgtgg atcaactgaa taaaggcctg ctccattgtg
     6541 ggatcgggtt gctcgtcgtt ggccgactgc gctttcaaat catccggcgt gccgctggcg
     6601 attaatttcc cgcggtacac caggccgatg cggtcgcaat attccgcttc atccataaag
     6661 tgggtggtga ccatcaccgt gacgcctttc tctaccatac tgttgatatg tagccaaaat
     6721 tcacggcggg tgaggggatc aacgccggac gtcggttcgt cgagaaacag aatgtccggt
     6781 tcatgcatca gcgaacaggc cagcgccagc cgctgtttaa aacctaatgg cagttcatcg
     6841 gtcgcgtggg aggcgatact gttcaggccg aacgcctcgc tcatacggga gattttttcg
     6901 ttctgcgccc gaccgcgtaa gccatacaca ccagagaaaa agcgtaaatt ctgttcgacc
     6961 gtcaggttgc cgtagagcga aaatttttgc gccatatagc cgagatgctg gcgcgcttta
     7021 ccagaactct ctttcagatc catgcccagc accagcgcct gaccagaggt cggtaccagc
     7081 aaaccgcaca tcatcttaaa ggtggtcgat ttacccgcgc cgtttggtcc gagcaaacca
     7141 aaaatctccc cgcgtttaac ggcaaagttg acgtgatcgg tggcggcaaa atcaccaaat
     7201 ttcttggtta gttccttcgc ttcgatcacc gtctcgccgg gtgtgccttc taccgtatgt
     7261 aatattgcgc ccagcggcga ttccgaggtt ccggcaccgc ccagcaaatc aataaacgca
     7321 tcttcaaaac gcggcgtagt ttcgttgatg tcgatttccg gcatcccgtc ggcatggcga
     7381 atatcgtctg gcgtggtctc ttttttgagg atcagacgta ccgatttccc ctgaatcatg
     7441 ccgtcgctga cctgcggcag tttcaatgcc cgttgcaaca gtttgcggtt gccctcgtgc
     7501 gggctggtca tcagaaagct gcgtccggcc atggtttgag tcagggccgt cggttctccc
     7561 tgatacagca gctcgccttc gttcatcagt aacacgtcac ggcactgctc ggcttcgtcg
     7621 agatacgagg tactccagag gattaacatc ccttcgcccg ccagctcatg caccatctgc
     7681 cacagttcgc gccgtgagat agggtcaacg ccgacgccgg gttcatcgag cagcaacact
     7741 ttcggttcgc ccaccagggt acaggccagg ccaagttttt gtttcatccc accggagagc
     7801 ttgcccgcca ggcgtccggt aaacgggcca agagacgtaa actccagcag gcgagcaaaa
     7861 gtttgcttcc gtgcctcgcc ggtgacgctg cgcaaatccg cgtacagatt gaggttctcc
     7921 atcaccgtca gatcttcata cagaccaaat ttctgcggca tatagccgag cacggcgtgc
     7981 agcgcagcgt catttttgat cggatcaaag ccaatcaccg tggcactgcc gctgtcgggt
     8041 ttcagtaatc ccgccagcat ccgcatcagc gtggttttgc ccgcaccgtc cggcccaacc
     8101 agccccgtca cataaccggc gtgaatggtg cagtcgagcg gtgcgacggc gggcttgtcc
     8161 atgcccggaa agcgtttttc caggccgttc agcgtgataa cggcatcatt catgtcctgc
     8221 ctcgtcaccg aattgcaccg ttaccggcat tccctggcgt aacgcatcat cggcgtcggt
     8281 caccacaata cgcaggcgat agacgaggtc ggtacgcaga tccggtgttt cgacggtttt
     8341 cggggtaaat tcagcagtcg gcgaaacgaa accaatctgc ccgtgatacg gcttgtccgg
     8401 gcgaccatcg gtataaagca acactttgcg ccccggctgg gcctggtcaa gattacgttc
     8461 atcaacatag gcgcgtaccc acaccggacg cgtcagcgag acggtaaata ccgtgccacc
     8521 ttcattaagc accgtgccgg gctctaccgc gcgcgttaac agcgtgccat cagacggagc
     8581 gaccagcgtc gaatcctgca aattcagctc cgcctgcgcc agttgcgcct gcgcttgttc
     8641 gaggctggct ttcgcctggg cgatgtcctg ttcgcggtta ccggaacggt actgacgcaa
     8701 tttatcctgc gctgatttca gcgtggcctg cgcctggtcg cgcgaggagc gggcattttc
     8761 caggtcattt gccgaaatgg tgcggctttt ccacagtcct tgctggcggt tatagaagtt
     8821 ctgcgcatag tcataggcag cttgcgcctg tttcaccgct gcggcggcct gagcgatttc
     8881 ttcatctcga tacccggcaa gcatcaggtc atactgcgcc tgagccaccg aaacgcccgc
     8941 tttcgcctgc atcagggcaa tctcatacgg cttgtgatcc agttcgccca gcacctggcc
     9001 cgctttgatc gcatcacctt cgtccaccgc cagcgactca acgcgccccc caacacggaa
     9061 actaagattc accgtacgaa tatccacgtt gccatacagc gtcaggccgt tatcctggcg
     9121 gctttgatac caccagtagc ctccggcaac cacggcggca agtactacta ccgccagtcc
     9181 gatcacgaca ggttttttca tcactacaaa ctcctttgcg ataatccttg caaaatcagg
     9241 tcaatatgac aggtcaccgt ctggttgatc agttcggtct tttcttcatc gaacgcggtc
     9301 cagccggtac gtaacagaat agtttctttg ccaagacgaa acgccagaat ctcaccaatc
     9361 aacgcatggg tatgaaggat catgcgggta tcgctagcgt cgcagccggt ccaggcggca
     9421 atcaggcgtg taaggtgact gtgtagcgga ctaatcacct gttcgtgcac caggtggtag
     9481 gctgccgtgg gagagagctg ctcacgggag ataaatttgc tgaggttgac ggtgtcatcc
     9541 tgggtgagca gcttaatcat gtttctgcag gcgcgaagga tcagttcacg gatggctgcc
     9601 cgatccggct gtggttgtgc gaacaaacgt tcggcttctt cggcgtgtgg acggaactgc
     9661 tcgccaataa aatcagcaat ccactgggcg caggcgaggt acaaatcttc tttcgaaccg
     9721 aagtagtagg tgatggctgc gatattctgc ccggcctggg cggctatctc gcgggtggtg
     9781 gcgttcattc catattcacc aaactgtgcc agtgccgcag caatcagctg ttttttcgcc
     9841 tgttcaccct tgattgtcat ggcaggatta ttcatcgcac agtccattct taatcaaatg
     9901 attgattaag attatgactc cataggggag ttgtccagta tggctaagaa ttttagctac
     9961 gccagtcaca gggataattt atgcgctgcg tcacaaaaac tgctacactc cgctccctca
    10021 tgacattgtg gtttttgtca ttttcctttt cagtatctcc ctgaaaacta caccggtaac
    10081 ggtcggggcg gttcggagta gttatgtctt tcgattcttt gggtttaagc cctgatatcc
    10141 tgcgcgccgt tgccgagcag ggttaccgtg aacccacccc tattcagcag caggcgatcc
    10201 ctgcggtgct ggaaggccgc gacctgatgg ccagcgccca gaccggtacc ggcaaaacag
    10261 cgggctttac gctgccgctg ttgcaacacc tgatcactcg ccagccgcac gccaaagggc
    10321 gtcgtccggt acgcgcgctc attcttaccc cgacccgtga actggcggcg cagattggcg
    10381 aaaacgtccg tgattacagc aaatatctga acattcgttc gctggtggtg tttggcggtg
    10441 tcagcattaa cccacaaatg atgaagttgc gcggcggcgt tgatgtgctg gtggcaacgc
    10501 cgggacgtct gctggacctg gaacatcaaa atgcagtgaa gctggatcag gttgaaatcc
    10561 tcgtccttga tgaagctgac cgcatgctcg acatgggctt tatccacgat atccgtcgcg
    10621 tgttaacaaa actgccagcg aagcgccaga acctgttgtt ctccgcgacc ttctctgacg
    10681 atattaaagc cctggcggaa aaactgttgc acaacccgct ggaaatcgaa gtggcacgcc
    10741 gcaatactgc gtctgatcag gtgacccagc acgttcactt tgtcgataag aaacgcaaac
    10801 gcgaattgct gtcgcatatg attgggaaag ggaactggca gcaggtgctg gtgtttaccc
    10861 gtaccaaaca tggcgctaac catctggctg aacagcttaa taaagacggc atccgtagtg
    10921 cggcgatcca cggtaataaa tcgcaaggtg cgcgtactcg tgcgctggcc gattttaaat
    10981 cgggcgatat tcgtgtactg gtggcaactg acatcgctgc gcgcggtctg gatatcgaag
    11041 agttgccgca cgtcgtcaac tatgagttac caaacgtacc ggaagattat gttcaccgta
    11101 tcgggcgtac tggccgtgcg gcggcaactg gcgaagcatt gtcgctggtg tgtgttgatg
    11161 aacacaaact gctgcgtgat atcgaaaaac tgctgaaaaa agagatcccg cgcattgcga
    11221 ttccggggta tgagccggac ccgtcaatca aagccgaacc gatccagaac ggtcgccagc
    11281 aacgtggcgg cggtcgtgga cagggcggtg gtcgcggtca acagcaacca cgccgtacgg
    11341 aaagtggtgc gaagtctggt aacgcgaagc ccgcagaaaa accgtctcgt cgtctcggcg
    11401 atgccaaacc ggcaggcgaa caacaacgtc gccgccgtcc gcgtaaacct gccgctgcgc
    11461 agtaatcttt tatgccgggc tatgcccggc atgagactga tgacaaacac aaaactgcct
    11521 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaatgt gcatgttttt
    11581 gtaggccgga taaggcgctc gcgccgcatc cggcatttca caacaagcac ttgtcagcca
    11641 tttgcgagcg cggcaaaaag atttctctgt tttaacgccc acaagccacc cgatgcgtta
    11701 cttctccgca gccaattgtc cgcgcaacgc cattaaaagg taccccaggc gattcttgcc
    11761 ctgaccatta ccgccatctc cccagtaagc atcattttgc gtatgctcaa ccagttttgc
    11821 ggacgcggtt gcccgcaaga gtgtacgcag ttctgcatgt tgttcgaatt tagcgcgaag
    11881 cgctttacgc atcatcctgc tggttattgc gagccgcttt cccgaaacag aaaaaccatt
    11941 acccctgaaa accgaaaaat gccacaatat tggctgttta tacagtattt caggttttct
    12001 catggcatta accgccgcgc ttaaagcgca aattgccgcc tggtataagg cgcttcagga
    12061 acagatcccc gactttattc cccgtgcgcc gcagcggcag atgattgcgg acgtcgccaa
    12121 aacgctggcc ggagaagaag ggcggcatct ggcgattgaa gcccctaccg gcgttgggaa
    12181 aacgctctcc tatttgatcc ccggcatcgc cattgcccgc gaagagcaaa aaacgctggt
    12241 ggtaagtacc gccaacgtgg cattgcagga tcagatctac agcaaagatt taccgctgct
    12301 gaaaaagatc attcccgatc ttaaattcac tgccgctttt gggcgtgggc gctacgtttg
    12361 cccgcgtaat ctgacagcgc tcgccagcac tgagcccacg caacaggatc tgctggcgtt
    12421 tcttgacgat gaactgacgc cgaacaatca ggaagagcaa aaacgttgtg cgaagctgaa
    12481 gggcgatctc gacacttata aatgggatgg tctgcgcgat catactgata tcgctataga
    12541 tgatgatctc tggcgtcgtt tgagtaccga caaggccagc tgcctcaacc gcaactgtta
    12601 ttactatcgc gaatgcccgt tttttgtcgc tcgtcgggaa attcaggaag cggaagtggt
    12661 ggtggcaaac catgcgctgg tgatggcggc gatggaaagc gaagcggtat tgcctgaccc
    12721 gaaaaattta ctgctggtgc tggacgaagg tcatcacctg ccggatgtgg cgcgggatgc
    12781 gctggagatg agcgccgaaa tcaccgcgcc gtggtatcgg ctacagctgg acttgttcac
    12841 gaaactggtc gctacctgca tggagcagtt tcgcccgaag accatcccgc cgctggcgat
    12901 ccctgaacgt ttgaatgcgc attgtgaaga gttgtatgag cttatcgcct cgttaaacaa
    12961 cattctcaat ctctacatgc ctgccgggca ggaggcagag caccgttttg cgatgggcga
    13021 actgccagat gaagtgctgg agatctgcca gcggctggca aaactcaccg agatgctgcg
    13081 tggcctggcg gagttatttc ttaacgattt aagtgagaaa accggcagcc atgacattgt
    13141 acgtctgcat cggttgattt tgcagatgaa ccgcgcattg gggatgttcg aggcgcaaag
    13201 caaactctgg cggctggcgt ctctggcgca atcttccggt gcgccggtga ccaaatgggc
    13261 gacgcgggaa gagcgcgaag ggcagctaca cctttggttt cactgcgtgg gaatacgtgt
    13321 cagcgatcag ctggaaaggc tgctgtggcg cagtattccg cacattattg ttacctccgc
    13381 aaccttgcgt tcgctgaaca gtttttcgcg tttgcaggag atgagtgggc tgaaagagaa
    13441 agcgggcgac cgttttgtgg cactggattc cccttttaac cactgtgaac agggcaaaat
    13501 tgttattccc cggatgcgcg ttgagccttc catcgacaac gaagagcagc acattgccga
    13561 aatggcggcc tttttccgtg aacaggtgga gagcaaaaaa catctcggta tgttggtgct
    13621 gtttgccagc gggcgtgcga tgcagcgctt tctcgactat gtgacggatt tacgcctgat
    13681 gttgctggtg cagggcgatc agccacgtta tcgcttagtt gaactgcacc gcaaacgcgt
    13741 tgccaacggt gagcgtagcg tgctggtggg cttacagtcc tttgccgaag gtcttgatct
    13801 gaaaggcgat ctgctcagcc aggtgcatat ccacaagatc gcttttccgc ccatcgacag
    13861 cccggtggtg atcaccgaag gtgaatggct gaaaagcctc aaccgctatc cgtttgaggt
    13921 gcaaagcctg ccgagcgctt cgtttaacct gattcagcag gttgggcgac tgattcgaag
    13981 ccacggttgc tggggggaag tggttatcta cgacaaacgc ttgctgacca aaaattatgg
    14041 caagcgacta ctggatgcat taccggtatt tccgatagag caaccggaag tccctgaagg
    14101 tatagttaaa aagaaagaaa aaacgaaatc cccacgccgt cggcggcgtt aatgatgtga
    14161 gtcaggtaag gagtcgtaaa tggactatcg caaaatcatt aaagagatcg ggcgcgggaa
    14221 aaaccacgcg cgcgatttag accgggatac tgcgcgcggt ctgtatgccc atatgctcaa
    14281 tggtgaagtc cctgaccttg agttgggcgg cgtattgatt gcgctacgta tcaaagggga
    14341 aggggaagca gagatgctcg gcttttacga agccatgcaa aatcacacca tcaagctgac
    14401 gccgcctgca ggcaagccga tgccgattgt catccccagt tacaacggcg cgcgtaaaca
    14461 ggccaacctg acgccgttgc tggcgattct gctgcacaaa ctcggttttc ctgtggtggt
    14521 tcacggggtt agcgaagatc caacccgcgt gctgaccgaa accattttcg aactgatggg
    14581 tattacgcca acgttgcacg gcgggcaggc gcaggcgaag ctcgacgaac atcaacctgt
    14641 ctttatgcct gtcggcgcgt tttgcccgcc gctggaaaaa caactggcga tgcgctggcg
    14701 gatgggcgtg cgtaacagtg cacataccct ggcgaaactg gcgacgccat ttgccgaagg
    14761 tgaggcactg cgtctttcca gcgtttcgca tccggaatac attggacgcg tcgcgaagtt
    14821 ctttagtgat atcggcgggc gggcgctact tatgcatggt actgaaggtg aagtgtatgc
    14881 taatccgcag cgctgcccgc agatcaatct cattgaccgt gaagggatgc gggtactgta
    14941 tgaaaaacag gacactgctg gtagcgagtt actgccacaa gcaaaagatc cggaaaccac
    15001 ggcgcagtgg attgagcgtt gccttgctgg cagcgaaccg attcctgaat cgctgaaaat
    15061 ccagatggct tgctgcctga tggctacggg tgaagcggca actatcaacg acggcctggc
    15121 gcgcattaat caggcatttt aatttttatc tcagccggat gagccatgtc atccggcttt
    15181 taccccaccg caatatgaaa ttcctgcatc tttattgacc ttcccgcgcc cggcgtgcag
    15241 cataaaaaaa caacaaacac ataacataaa caggagttaa ccatggaaag tggtcatcgc
    15301 tttgatgctc agacgctgca cagttttatt caggctgtat ttcgtcagat gggtagcgag
    15361 gaacaggaag cgaaattagt tgccgatcat ttaatcgcgg caaacctggc agggcatgat
    15421 tcacatggta ttggcatgat cccaagctat gtgcgttcct ggagtcaggg gcatctgcaa
    15481 attaatcatc atgccaaaat cgttaaagag gcgggggcgg cggtcacgct cgatggcgat
    15541 cgcgcatttg gtcaggtcgc ggcacacgaa gcgatggcgc tggggattga gaaagcgcat
    15601 cagcacggca ttgccgccgt ggcgctacat aactcgcatc atatcggccg tatcggttac
    15661 tgggcggagc agtgtgcagc ggcggggttt gtctctatcc actttgttag cgtggtcggt
    15721 attccaatgg tcgcgccgtt ccacggtcgc gacagccgct ttggcaccaa tccgttctgt
    15781 gtggttttcc ctcgtaaaga taatttcccg ctgttgctcg attacgccac cagcgccatt
    15841 gcatttggca aaacccgcgt cgcctggcat aaaggcgtcc ccgtgccgcc aggttgcctg
    15901 attgacgtta acggcgtgcc gacgaccaat ccggcggtaa tgcaggagtc gccgttgggt
    15961 tcgctgttga cctttgccga acataaaggc tacgcccttg cagcgatgtg tgaaattctt
    16021 ggcggggcgc tttctggcgg taaaacgacg catcaggaaa cgttacaaac cagtcccgat
    16081 gccattctta actgcatgac tactatcatt atcaacccgg aactgttcgg cgcgccggat
    16141 tgtagtgcac agaccgaagc ctttgccgag tgggtgaaag cctcgccgca tgatgaagat
    16201 aaaccgattt tgctaccggg cgagtgggaa gtgaacacgc gtcgcgaacg gcaggagcag
    16261 gggattccac tggatgcggg aagctggcag gccatttgtg atgcggcgcg gcagattggt
    16321 atgccggaag agacgttgca ggctttctgt cagcagttag ccagctaaaa aaaacccgtc
    16381 cagtggcgga cgggcgaaca agggtaacat aggatcaatg agggttagag catatgcgtc
    16441 tgtcggcaaa cagacaggga aatacttgtg ctgaacgtag cgtaaacgcc tgatccagcc
    16501 aacggttcgg attttgtagg tctgataaga cgcggtagcg tcgcatcagg catctgttgc
    16561 cggatgcggc gtaaacgcct tatctgtcct acgaattact tataaattac cgcagtaccg
    16621 cttaatttat tgttgttggt ggcggaagta atgctgtatc cactggcacc ggccgccgcg
    16681 gctttctcag ccagtttcgc ttccagggca tcgagggtgg atgcgccatc ggcagaaacc
    16741 acgccgattt tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg
    16801 ccaaatgaca gagttgaaag agccatagca gcaacaacaa tattgatagt tttcataatt
    16861 aatctctcgc aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac
    16921 gtttttttac gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg
    16981 atatctaaca tactgataaa ccatttattt gttgtattta tgaatccgtt ttgtaccgcg
    17041 tggtgctcac ggcagcgtta aaggcacgga atgccacgca aaatacaaat tacactcact
    17101 aaacatcccg tcagttgcag ttaacgtaac tggctgtctt tcgaacctct gcgattatat
    17161 cctgtataag ctggtttttg taaatctttc tcctgttggc aatgaatgca taagcgcacg
    17221 ccaggaatgg cttcccgacg ggcctgcggg ataggggcac cgcactcttc acattcatac
    17281 aggctttcgc cacgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg
    17341 ttgatctgtt cgttgacggc gtcatcatta gcccaaccgg atgccatatc gtcctcccca
    17401 tatcaatact tgtacagtta agtgtagcta atatagggac gaactcgggc agttcaagcg
    17461 tcagatctcc gaccattccc gcagcagatt atgataaaga ttgagcagtg acaggatctc
    17521 ttcactttcg ccgtagcggc ttttcagcga ctgaatattg ttgtccagtt caaacagcat
    17581 ggcgcgcttt ttatcatcac ggatcatcga ctggatccac ataaatgatg ccactcgcac
    17641 gccgcgggtt acgggtgtca cgcaatgcag gctgctggag ggatacaaca ccagatcgcc
    17701 tgccgggagt tttacccgat gttgcccgaa ggtgtcatta acgaccagtt cgccgccgtc
    17761 gtagctttct ggatcgctta aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt
    17821 ttgtggatgg ctgcgtactg cgccatccac atgaaaacca taggtttcat tgttctgata
    17881 gcgattaaac agcggcgtgg aaagggtacg cggcaaggcc gcggcaaaga acaaagcatg
    17941 ttggttaacc gcgttcagca cctcattttg cagggcggcg tacagggcgc tgcgggtgtc
    18001 tacttgttga ttgttcttaa cttgcgcacc ttgtgcgccg gtggtgacgc gcccatccac
    18061 ccattcggct tgttccaggt gttcgcgaaa acgggcgacg tcctgtggcg ataacacgcc
    18121 gggaatgtgg tacatcatac aagttctcca aaaagtgggg cctgcgcccc acaccgattt
    18181 ttaacagcag gcaatggtgc tcaggtgccg gatgcggcgt gaacgcctta tccggcctac
    18241 caaatgatgt gaattcagtg tattgcacaa ccttgtaggc ctgataagcg cagcgcatca
    18301 ggcagtcctg ctctcgccat caacattagt gagccttgcg ccccacatct gaatcagaaa
    18361 tgcatattag ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg gtagccgctc
    18421 ttattgattg aggcgacgta atcggtatca aacaggttgt agacgtttag ctggaagtcg
    18481 agattgcgat taacccgata ccccagtttg gcgtcggcga cccaataacc ttcggtaaac
    18541 gctggcgttc ccaccgcgcc gtctgaacct ttatgcatac tgccgatata ccgtgcgccc
    18601 gcaccaacag aaatatcatc ggttgcctga tactggctcc ataaggtgaa ggcgtgttcc
    18661 ggggtatacg gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt tttgatagtt
    18721 gctttttgct gggtatagcc gccaatcatc tgccatgcgg gggtgatatt cccggccacg
    18781 gatatctcgt agccttcgac acgtttctta ccatattgcg aataagtccc gtcatcattt
    18841 tgctcaactt cattttcaat atcagtacgg aacagcgcgg cggtaagtag caggcgttta
    18901 tccagaacct gccatttggt gccaatctcg ctggtgttgg ctttttgtgg tttaaaatcg
    18961 gtgcggttgg cactgttacc gctgccagac tgcgcaaggg cgaagttgtt gccgcccggc
    19021 ggctgctggg aaacggcata gttaatatag acattgccgt tttccgtcag gtgatacagc
    19081 gccccggctt tccagttcac cagattgccc gacttggcgg tgtcgacggt ggtgaccgga
    19141 gaaccttttg ccacgccagc agggcaggtg atggcaccgc gcccgctgcc gccgcaggcg
    19201 gtagcactgt catattcagt atgataatta tccagacgga tcccgccgtt cagctcaaaa
    19261 tcacgggtga tttgcagcgt atcgaaggca taaattgcga aggtatccgt ctgaccattg
    19321 gcgtttgcgc cgttgcgcgt caggccgccg ggatgaatgc tgctgtcagg atgataaata
    19381 tttaccgccg gtaacgtcac cggattaacg ccatagttag tctgtgtttc gcgggtaaat
    19441 tccacgccgg tactgagatc atgaccgata gcgccggtat agaatgtcga ggtcaggttg
    19501 gtctggttgg tgagaatttt gttactcaca tctttggtat tcgccgtgcg tgaccacgtc
    19561 cagctattca catcgctggt gggctgggta atattcgacg ctccacccat aatcgccgtc
    19621 atcaggtaat cctgctttac gcgcgaccaa cgcgtagtgt tgcgaatggt ggcgttatcg
    19681 ttgatgtcgt gctcaaaacg catggtagcg gtgtcggtga tcgaatcgtc gtaatcggaa
    19741 tctgtgccgt aaaagttatg agtatcaact tttccggaat gattcagggc tgctgttccc
    19801 gcagatgggg cagaataacc cggtaaaccg atagtcggaa tgccgccgtc tggcgtgttg
    19861 tgctgggtga catgcagata attaagatac aaacgattcg ctgtaccaag gccaaaagcg
    19921 acagaagggg cgacgccgta acgctcattt ttgactttgt cgcgtccggc atcgtgcgtt
    19981 ttttcgccca ttacattcag gcgcaccgca gtggtatcac caatgacctg attgacgtcc
    20041 agcgtgccgc ggcggaacca ggcgctgcca atactggcgg aggcgtcaat gccggaatca
    20101 ttgcgcggct gtttgctgat catattgatc gagcctgtcg gtgcgctgcg cccgtagtcg
    20161 gtgccggacg gccctttaat tacttcgacc tgctcggtat tgaaggtgtc gcgcgagacg
    20221 ctgccgatat cgcgaatgcc atcgatataa atactgttag aggtatcggc accgcgcata
    20281 taaatggcgt cgccagtggt ggagttaccg ttctcacccg caaaaaacgc acccacgccg
    20341 g
//
LOCUS       DAZFRE010000086        20111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFRE010000086 DAZFRE010000000
VERSION     DAZFRE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.086"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            34..558
                     /gene="lpfA"
                     /locus_tag="ACHKUI_003664"
     CDS             34..558
                     /gene="lpfA"
                     /locus_tag="ACHKUI_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO6599788.1"
                     /translation="MKKVVFALSALALTSGSVFAADSGDGTVKFTGEIVDAACVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPSKPFEIKLEDCDITTLKKAKVSFSGVGDTDKPEL
                     ISVSTEAGAAKGVGIGIYDNSNTLVAMNSGEASTDLKKGQTVLYFTANYVSTLAAVTT
                     GYGNAQVDFNLSYE"
     gene            640..1311
                     /gene="lpfB"
                     /locus_tag="ACHKUI_003665"
     CDS             640..1311
                     /gene="lpfB"
                     /locus_tag="ACHKUI_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO6599789.1"
                     /translation="MALTLWVLISQSALASGVGLSSTRVIYDGNKKEASLTVQNKNKS
                     EEFLIQSWVDDATGSKKTPFIITPPLFKLEPGKNNILRIVNVNPSLPQDRESVYWVNV
                     KAIPSQNEENEGKNVLQIAVRTRIKLFYRPAGLKGDIKSSPGELRFTRNGKQIKIDNP
                     TVFNITFSDVFINGHEIEKSGMVPAKGSLNINLPAGVNSASQIKYNTINDFGSSGEYL
                     TREIN"
     gene            1335..3911
                     /gene="lpfC"
                     /locus_tag="ACHKUI_003666"
     CDS             1335..3911
                     /gene="lpfC"
                     /locus_tag="ACHKUI_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO6599790.1"
                     /translation="MSWMVVSRTYTSLPFSLSVLALTVAGSFSATAGKFNPRFLEDTA
                     GINQHVDLSMYETDHGAQLPGTYRVSLIVNEQKMETRTLEFKAATESQRKEMGEFLIP
                     CLSRTQLADMGVRVDSFSALNLIPAEACVAFNEIIPQATSHFDFSEQKLVMSFPQAAM
                     QQVARGTVPESRWDDGVPALLLDYSFSGSNSSHDTKSYNRYIDENGNHHQVKNETSQT
                     NDSYYLSMRSGLNLGAWRLRNYSNWSYSNGEKQWDNIGTYVTRAIVPLKAQLTLGDTA
                     TPSDIFDSVQMRGALLASDEEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRAFV
                     QPGAFEINDLYPTANSGDLTVIVKEADGSEQRFIQPFSSVAIFQREGHLKYSLSAGEY
                     RAGNYDSANPRFGQFNAMYGLPFGMTTYGGALISKDYNAFALGLGKNFGSIGAISVDI
                     TQAKSTLNNNATDQGQSYRFLYSKSFASGTDFRLLGYKYSTSGFYTFQEATDVRSGAD
                     SDYGRYHKRSEIQGNLTQQLGTYGSVYFNMTQQDYWNDDGKRLSLATGYNGRIGRVNY
                     SIAYSWNKSPEWDENDQLWSFNVSIPFGRAWSNYRVTTDQDGRTTQQLGVNGTLLEDR
                     NLSYNVQEGYSSNGVGNSGNASLAYQGGAGNISVGYSYGKDYQQTNYSLRGGIVAHSE
                     GISLSQPLGETIGIVSAPGARGAKVLNNSGVSVDWQGNAVVPYLSIYRENDVSIRSET
                     LNDSVDMNSAFQTVVPTRGAVVRAHFDTRVGYRVLMTLIRQNEVSVPFGATATLVSDK
                     SEQISGIVGEDGQLYISGMPKKGDVKIVWGKDTSQQCVAKYELPIKKKSSGIISVTTN
                     CQ"
     gene            3927..4982
                     /locus_tag="ACHKUI_003667"
     CDS             3927..4982
                     /locus_tag="ACHKUI_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6599791.1"
                     /translation="MMKISSLFFVGSMLFTANNVMAADWGPCTSTSGVHDYPVNFDID
                     IMDASKNEAGTELKLIYSWTGVGQPFNFICECPDTYKTEKDTLIKVVSNLSTATRKIN
                     SEQYYRLTDELEVMTMIQGPSVMFRVPEANSQIPNLNNSSQDCKGNNLGQFGSNGYLS
                     FYIAKPVQGEVEIPYTLIATMYASKKKNTYRDPLSTVSIAGKLTFTQGCKIDAGTVLD
                     VPFGEYPSSAFKNKKGTIPVGGTEKEINLQFDCENITDGIQVSLRIEGNTSQADERAI
                     DMGNPDIGVLVMDPNGNVLKPNDTNSSVNLNLGSNDSQKHRDATIKLKAAPISTTGNA
                     PAAGQYSGVATIFLDMD"
     gene            4988..5518
                     /gene="lpfE"
                     /locus_tag="ACHKUI_003668"
     CDS             4988..5518
                     /gene="lpfE"
                     /locus_tag="ACHKUI_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO6599792.1"
                     /translation="MWRNSIRCLLPFLALAYFPGAEAKISLGEAGKLTFSLSVVAKGC
                     EFEAADLEVVMGKMMLMKPVTVGRVINEKNFVIELKDCDGISKAKVTMDGLPDTNDNS
                     LFALDAGGATGIALQIVDGKGTKQIPKVAGGTAIEWPVNGTTTQLNYKASYVVVNANA
                     TFGHANAMVNFSVEYE"
     gene            5782..7473
                     /gene="eptB"
                     /locus_tag="ACHKUI_003669"
     CDS             5782..7473
                     /gene="eptB"
                     /locus_tag="ACHKUI_003669"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6599793.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEASAN"
     gene            7562..7638
                     /locus_tag="ACHKUI_003670"
     tRNA            7562..7638
                     /locus_tag="ACHKUI_003670"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7596..7598,aa:Pro,seq:cgg)
     gene            8382..9989
                     /gene="dppA"
                     /locus_tag="ACHKUI_003671"
     CDS             8382..9989
                     /gene="dppA"
                     /locus_tag="ACHKUI_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6599794.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10296..11315
                     /gene="dppB"
                     /locus_tag="ACHKUI_003672"
     CDS             10296..11315
                     /gene="dppB"
                     /locus_tag="ACHKUI_003672"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6599795.1"
                     /translation="MLQFILRRLGLVIPTFIGITLFTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11325..12227
                     /gene="dppC"
                     /locus_tag="ACHKUI_003673"
     CDS             11325..12227
                     /gene="dppC"
                     /locus_tag="ACHKUI_003673"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6599796.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12238..13221
                     /gene="dppD"
                     /locus_tag="ACHKUI_003674"
     CDS             12238..13221
                     /gene="dppD"
                     /locus_tag="ACHKUI_003674"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6599797.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFQAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13218..14222
                     /gene="dppF"
                     /locus_tag="ACHKUI_003675"
     CDS             13218..14222
                     /gene="dppF"
                     /locus_tag="ACHKUI_003675"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6599798.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRQEKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14252..15523)
                     /gene="yhjV"
                     /locus_tag="ACHKUI_003676"
     CDS             complement(14252..15523)
                     /gene="yhjV"
                     /locus_tag="ACHKUI_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6599799.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSLVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            15999..16106
                     /gene="ldrD"
                     /locus_tag="ACHKUI_003677"
     CDS             15999..16106
                     /gene="ldrD"
                     /locus_tag="ACHKUI_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6599800.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16193..17872)
                     /gene="bcsG"
                     /locus_tag="ACHKUI_003678"
     CDS             complement(16193..17872)
                     /gene="bcsG"
                     /locus_tag="ACHKUI_003678"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6599801.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17869..18060)
                     /gene="bcsF"
                     /locus_tag="ACHKUI_003679"
     CDS             complement(17869..18060)
                     /gene="bcsF"
                     /locus_tag="ACHKUI_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6599802.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18057..19628)
                     /gene="bcsE"
                     /locus_tag="ACHKUI_003680"
     CDS             complement(18057..19628)
                     /gene="bcsE"
                     /locus_tag="ACHKUI_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6599803.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19911..20099
                     /gene="bcsR"
                     /locus_tag="ACHKUI_003681"
     CDS             19911..20099
                     /gene="bcsR"
                     /locus_tag="ACHKUI_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6599804.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5254 a         4676 c         5151 g         5030 t
ORIGIN      
        1 taagcatatt tatctatata aatggagtta ttcatgaaaa aggttgtttt tgccttgtca
       61 gcactggcgt taacttctgg ttccgtattt gcagcggatt ccggtgatgg cactgtgaaa
      121 tttacaggtg aaatcgtcga tgcagcttgc gtactgtcag ttgattctca gaatcaggaa
      181 gttgtactgg gtcaggtgca aaaatctgta tttgctgcag tcggtgataa atcaccatca
      241 aaacctttcg aaatcaagct ggaagattgt gatatcacca ctttgaaaaa ggcgaaagtc
      301 agctttagtg gcgttggtga taccgataaa cctgaactta tctctgttag caccgaagca
      361 ggcgcggcga aaggcgtagg tatcggtatt tatgataaca gcaacaccct ggttgcgatg
      421 aatagtggtg aagccagcac tgacctgaaa aaaggtcaga cagttcttta tttcaccgca
      481 aactacgttt ctactttagc tgctgtaact actggttatg gtaatgctca ggttgatttc
      541 aatctgtctt acgagtaata gttagtttca caattggcga agatgaattc tccgccaatt
      601 gtgttcaacg ttcatggata gaatgatgaa aaaaaattca ttgcgttaac tctttgggtg
      661 ttaatttcgc agtctgcact agcgagtggg gttgggctta gttctactcg ggtgatttat
      721 gacggtaata agaaagaagc atcgctaacc gtacaaaaca aaaataaatc agaagaattt
      781 cttattcagt catgggttga tgatgcaacg ggtagtaaaa aaacaccatt catcattact
      841 ccgccattat tcaaacttga gccaggaaaa aacaacatct tacgtattgt aaatgtgaat
      901 ccgtccttac ctcaggatcg tgaatctgtt tactgggtaa acgtcaaagc gattccttct
      961 caaaatgaag aaaatgaagg gaaaaatgtc ttgcagattg cagttcgtac tcgcatcaag
     1021 cttttttatc gcccagcagg tctaaaaggg gatattaaat cttcgccagg agaactacgt
     1081 tttacgcgaa acggtaaaca gataaaaatt gacaatccaa ctgtttttaa catcacgttc
     1141 agtgatgtat ttattaacgg acacgaaatt gaaaagtccg gtatggtgcc agcaaaaggg
     1201 tctttgaata tcaatttgcc tgcaggtgtg aacagtgctt cgcaaataaa atataataca
     1261 attaatgatt ttggctcttc aggtgaatat ctgactcgcg aaattaatta atagttttca
     1321 ggggaaccaa cttaatgtca tggatggtcg tttcgcgcac ttacacttct ttgccctttt
     1381 ctttatctgt attggcgctt acggttgcag gctctttttc tgcgacagct ggaaaattta
     1441 atccgcgttt tctggaagat accgcaggga tcaaccagca tgttgacctt tccatgtacg
     1501 aaactgacca tggcgcgcag ttgcctggaa catatcgcgt ttctttaatc gtcaatgaac
     1561 aaaaaatgga aacgcgcact ctggagttta aggcggctac agaatcccaa cgaaaggaga
     1621 tgggggagtt tctcatacct tgtttaagcc ggacacaact ggccgatatg ggcgttcgtg
     1681 tagacagttt ttcagcactt aaccttatac cagcagaagc ctgtgtggcg tttaatgaaa
     1741 ttatccctca ggcgaccagt cattttgatt ttagcgaaca aaagctggtg atgagttttc
     1801 ctcaggcggc aatgcagcag gttgctcgcg gtacagtacc tgaatctcgc tgggacgatg
     1861 gtgtgcccgc gttgcttctt gattacagtt tcagcggtag taacagttct catgatacaa
     1921 aaagttataa tcgctacatt gatgaaaatg gtaatcatca ccaggttaaa aacgaaacta
     1981 gccagactaa cgatagctac tacttaagta tgcgaagcgg ccttaacctt ggggcatggc
     2041 gtctgcgaaa ttacagtaac tggagctata gtaacggcga gaagcagtgg gacaatattg
     2101 gcacctatgt aacacgagcc attgttccgc tgaaagcgca actgacattg ggtgatacag
     2161 caacaccttc agatattttt gacagtgtgc agatgcgcgg agcattactg gcctctgatg
     2221 aagaaatgtt gccagacagc caacgtggtt ttgctccggt agttcgaggg atagcgaaaa
     2281 gtaacgcgga agtcagtatt gaacaaaatg gttacgtgat ttatcgagca tttgttcagc
     2341 caggtgcgtt tgaaattaac gatttatacc ccacagcgaa cagcggtgat ttaaccgtaa
     2401 ttgttaaaga ggctgatggt agtgaacaaa gatttattca gccattctct tcagtcgcta
     2461 ttttccaacg tgaagggcat ctcaaatata gcctttctgc tggtgaatac cgtgctggta
     2521 attacgatag cgccaaccca cgctttggtc agtttaatgc gatgtatggt ttaccgtttg
     2581 gtatgaccac ctatggtggc gcattaattt ctaaggatta caacgcattt gctctgggat
     2641 tgggaaaaaa ctttggctct attggtgcaa tatctgttga tattactcag gcaaaaagta
     2701 cattaaataa taatgcaacc gatcagggac aatcgtaccg ctttctttat tcaaaaagct
     2761 ttgccagcgg cacagatttc cgtttattgg gttacaagta ttctaccagt ggtttttata
     2821 ccttccagga agcaacagat gtgcgtagcg gtgctgacag cgattacgga cgttaccata
     2881 agcgtagtga aattcagggt aatttgacac agcaattggg cacctatggg tcggtttatt
     2941 tcaatatgac ccagcaggat tactggaatg atgacggtaa gcgcctttct ttggcaaccg
     3001 ggtacaacgg tcgtattggg cgtgttaatt acagtattgc ttacagttgg aataaaagcc
     3061 ctgaatggga tgaaaacgac cagttatggt catttaatgt ttccattccg tttggtcggg
     3121 catggagtaa ctaccgcgtg acaaccgatc aggatggtcg tacgacacaa cagcttggcg
     3181 tcaatggcac tctgttagaa gatcgcaatc ttagctataa cgttcaggaa gggtattcca
     3241 gtaatggtgt tggtaatagc ggtaatgcca gtttggcgta ccagggcggc gccggtaata
     3301 tcagcgtggg ttacagctat ggtaaagact accagcagac taactacagc ctgcgtggtg
     3361 gcattgtagc gcacagtgaa gggatctcgc tttcccaacc tttgggtgaa accattggta
     3421 tagtttcagc accaggtgcc cggggggcga aagtattaaa taatagtggt gtgagtgttg
     3481 actggcaagg taatgcggtc gttccttatc tgagcatcta ccgtgaaaat gatgtgtcca
     3541 ttcgcagtga aacgttgaat gacagcgttg atatgaacag cgcattccag acagttgtgc
     3601 caacgcgagg agctgtcgta cgggcgcatt tcgatacccg tgtgggttat cgagttctta
     3661 tgacattaat tcggcaaaat gaagtgagcg tcccctttgg tgcaactgca acgctggttt
     3721 cagataaaag tgaacagatt agcggtattg ttggtgaaga tgggcagtta tatatcagtg
     3781 ggatgcctaa aaaaggggat gtgaagatcg tatggggtaa agatacttca caacaatgcg
     3841 ttgcgaaata tgaactgccg attaagaaaa aatcgtcagg tattatttct gtaacaacaa
     3901 attgccaatg atcagggata gataatatga tgaaaatttc aagtctgttt tttgttggaa
     3961 gcatgttgtt tacggccaat aatgttatgg ctgcagattg gggaccctgt acatcgactt
     4021 caggtgttca tgattaccca gtcaattttg atattgatat tatggatgcc agcaaaaatg
     4081 aagcgggtac tgaattaaaa ttaatttact catggactgg tgttgggcag ccttttaatt
     4141 ttatttgtga gtgtccggat acttataaaa cggaaaagga tacactgatt aaagtcgtga
     4201 gtaacttatc tacggcaacg cgaaaaataa acagtgagca atactaccgg ttaaccgatg
     4261 agctggaagt aatgaccatg attcaaggtc caagcgtgat gttccgcgtt ccggaggcca
     4321 atagtcagat ccctaattta aataactctt ctcaagactg taaaggtaat aatctggggc
     4381 aatttggcag taatggctat ctgtcatttt atatagcaaa gcctgttcag ggtgaggtgg
     4441 aaattcccta tacgcttatt gcaacaatgt atgcctcaaa gaaaaaaaat acatacaggg
     4501 atccactttc tacggtcagt attgcaggaa aattgacctt tacacaaggc tgtaaaattg
     4561 acgccggtac ggtattggat gtccccttcg gcgagtaccc atcttcggcg tttaaaaata
     4621 aaaaaggaac cattccagtt ggagggactg aaaaagagat taacctccag ttcgattgtg
     4681 aaaatattac cgatggcatt caggtatctc taaggattga aggaaacaca agtcaggctg
     4741 acgagcgtgc catcgatatg ggtaacccag acattggtgt gttggttatg gatccaaacg
     4801 gcaatgtctt aaagcctaac gataccaaca gcagtgttaa cttaaacctc ggttcgaacg
     4861 attcccaaaa acatcgcgat gcaacaatta aactgaaagc agcaccaatc agcacgaccg
     4921 gaaatgctcc agcggcaggg caatatagtg gggttgcaac aatctttctg gatatggatt
     4981 agtcatcatg tggagaaata gtatacgttg cctgttaccc tttttagctc tggcatattt
     5041 tcccggcgct gaagctaaaa taagcctcgg tgaagcagga aaactgacgt tttctctttc
     5101 agtcgtggcc aaagggtgtg agtttgaagc cgctgattta gaagttgtca tggggaaaat
     5161 gatgttaatg aagccagtca cggtcggaag agtcataaac gaaaaaaatt tcgtaatcga
     5221 attaaaagat tgtgacggaa tatcaaaagc taaagtaaca atggacggcc ttcctgacac
     5281 caatgataac tctctttttg ctctggatgc cggaggcgca acgggaatag ccctgcaaat
     5341 tgttgacggc aagggcacaa aacagattcc gaaagtcgct ggtggaacgg cgatagagtg
     5401 gccagtaaat ggaacaacaa cccaactgaa ctacaaagcg agttatgtcg ttgtaaacgc
     5461 caatgcaacg tttggacatg caaatgccat ggtcaatttc tccgtggagt atgagtaacc
     5521 agtttcggac ttttggcaga aaaagaggag attactcctc ttttttacaa gaacagttaa
     5581 ttattggtct gcattctgac ttgctttttg taaatatctg tagctaaaac tcacttcatt
     5641 gccacttttc cttctctctg tggtctactt aacgacatga gcagaggata gcgttcacta
     5701 ctccggcatt ttatcctgat actcccactg taaaaacgga gtgctggata ttttttcccc
     5761 tttaccccag ggtttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc
     5821 tttttgcttg caatctatat tggccttttt atgaatggcg cggtttttta ccgccgcttc
     5881 ggcagctatg cgcacgattt taccgtctgg aaaggtattt ctgctgttgt tgaactggcc
     5941 gccaccgtac tggtgacctt ctttttactg cgtcttcttt cgctgtttgg ccgccgcagc
     6001 tggcgtattc tggcatcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc
     6061 ttccttaatg tagtcattgg ttatggcatc atcgcatccg tcatgaccac cgatatcgac
     6121 ctgtcaaaag aagttgttgg tctgaacttt attctctggt taatcgccgt aagcgcgttg
     6181 cctcttatcc ttatctggaa taaccgctgt cgttacacct tgctccgaca actgcgaacc
     6241 ccagggcaac gtattcgcag cctggcggtc gtcgtactgg caggtattat ggtttgggca
     6301 ccgattcgtt tgctggatat ccagcagaag aaagtggaga gggcgacagg tgttgatttg
     6361 ccgagttatg gcggcgtcgt cgcgaactct tatctgccat caaactggct ttcggcatta
     6421 ggtctttatg cctgggcgcg ggtcgatgaa tcttccgata acaattctct ccttaaccct
     6481 gcgaagaagt ttacgtacca ggctccacaa aacgttgatg atacatatgt cgttttcatt
     6541 attggtgaaa ccacgcgctg ggatcatatg ggcattttcg gctatgaacg caataccaca
     6601 ccgaagctgg ctcaggagaa aaatctggcg gcgttccgtg gttactcctg tgatactgca
     6661 accaaactct cactgcgttg catgtttgta cgtcagggtg gagcagaaga taatccgcag
     6721 cgcacattaa aagaacagaa cattttcgcg gtattgaagc agttaggatt cagttctgat
     6781 ctctacgcta tgcaaagcga aatgtggttc tacagcaata cgatggcgga caacattgct
     6841 tatcgtgagc agattggtgc ggagccacgt aaccgtggca aaccggtaga tgatatgttg
     6901 ctggtagacg aaatgcagca atcgctgggg cgcaacccgg atggtaagca tctgatcatt
     6961 ctgcatacca aaggctcaca ctttaactac acccagcgtt acccgcgcag ctttgcgcag
     7021 tggaagccgg aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactca
     7081 tacgacaact cggtgaccta tgtggatcac tttatttcca gcgtgattga tcaggttcgc
     7141 gataagaaag cgattgtgtt ctatgcagct gaccacggtg agtcaattaa tgaacgcgag
     7201 cacctgcacg gcacgccgcg tgaactggca ccgccggaac agttccgtgt gccgatgatg
     7261 gtctggatgt cggataaata tctggaaaat ccggtcaatg cgcaggcatt tgcgcagctg
     7321 aaaaaagaag ccgacatgaa agtgccacgc cgtcatgtag agctgtacga caccatcatg
     7381 ggttgtcttg gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgtcac
     7441 atcccgcagg caaaagaggc gtcggctaac taggcctgct gactttctcg ccgatcaaaa
     7501 ggcattttgc tattaaggga ttgacgaggg cgtatctgcg cagtaagatg cgccccgcat
     7561 tcggtgattg gcgcagcctg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga
     7621 atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg agcaggcttt tttgcatctg
     7681 tagctcatga ggaagagaac ctccgggggc ggggaggttc gactcgacgt aggcctgata
     7741 agacgcgcca gcgtcgcatc aggcaaatgt accggcccat tgtcggatgc ggcgcgaccg
     7801 ccttatccga tctacacacc acacatttcc ctgccaaatt tgcgccccat atctcactat
     7861 ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta aatctttttt
     7921 tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt catggtatta
     7981 atcgctcaga tatccacctt tacctcagat ttttttcatt aaatgcataa aacttaatca
     8041 ccaaatgttg ctaaatatta agctgattgt tctggttgtg ttggcgtagc acaaatttct
     8101 ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct caccgttggt
     8161 gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg taggtcacga
     8221 ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg gcatccccac
     8281 ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga caacccactg
     8341 caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt tccttgaaaa
     8401 agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca gcaagtgttc
     8461 aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac ccgcagctgt
     8521 ttacctccgg caccacctat gacgcctctt ccgtaccgct ttataaccgt ctggttgaat
     8581 ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga aaagtgggaa gtcagcgaag
     8641 acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcacgac aataaagaat
     8701 tcaaaccgac gcgcgaactg aatgccgatg acgtagtgtt ctcgttcgat cgtcagaaaa
     8761 acgcgcaaaa cccgtaccat aaagtctctg gcggcagcta cgaatacttc gaaggcatgg
     8821 gcttaccaga gctgatcagc gaagtgaaaa aggtggacga taacaccgtt cagtttgtgc
     8881 tgactcgccc ggaagcgccg ttcctcgctg acctggcaat ggacttcgcc tctattctgt
     8941 caaaagaata tgctgacgcg atgatgaaag ccggtacacc ggaaaaactg gacctcaacc
     9001 caatcggtac cggcccgttc cagttacagc agtaccagaa agattcccgt attcgctata
     9061 aagcgtttga tggctactgg ggcaccaaac cgaagatcga tacgctggtc ttctctatta
     9121 ctcctgacgc ttccgtgcgt tacgcgaaat tgcagaagaa cgaatgccag gtgatgccgt
     9181 acccgaaccc ggcagatatc gcccgcatga agcaggataa atccatcaac ctgatggaaa
     9241 tgccggggct gaacgtcggt tatctctcgt ataacgtgca gaaaaaacca ctggatgacg
     9301 tgaaagttcg ccaggctctg acctacgcgg tgaacaaaga cgccatcatc aaagcggttt
     9361 atcagggcgc gggcgtatca gcgaaaaacc tgatcccgcc aaccatgtgg ggctataacg
     9421 acgacgttca ggactacact tacgatcctg aaaaagcgaa agccttgctg aaagaagcgg
     9481 gtctggaaaa aggtttctcc atcgacctgt gggcaatgcc ggtacaacgt ccgtataacc
     9541 cgaatgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg ggcgtgcagg
     9601 ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg tgcgaaagat ggcgagcatc
     9661 agacggtaat gatgggctgg actggcgata acggggatcc ggataacttc ttcgccaccc
     9721 tgttcagctg cgccgcctct gaacaaggct ccaactactc aaaatggtgc tacaaaccgt
     9781 ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca caataaacgt gttgaactgt
     9841 acaaacaagc acaggtcgtg atgcacgatc aggctccggc actgatcatc gctcactcca
     9901 ccgtgtttga accggtacgc aaagaagtta aaggctatgt ggttgatcca ttaggcaaac
     9961 atcacttcga aaatgtctct atcgaataat taaagccatg caagactgat ggcaaaggca
    10021 aaactgcctg atgcgctccg cttatcaggc ctacgaaaac tctgcaatgt attgagtttg
    10081 cacgattttg taggtcggat aaggcgtttg cgccgcatcc ggcataaaca aagcgctctt
    10141 tgtcaacaat ctgcataccc ggtggcgctg tgcctctgcg tgcagcgcca tccggcagca
    10201 atacttcatt ccctgttcca caagggcggg aattgatttg tgagcaatac agacacgcag
    10261 ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt ctccgacgtt
    10321 tgggactcgt catccccacg tttatcggta ttaccctttt cacatttgcc tttgtccaca
    10381 tgatcccggg cgatccggtg atgatcatgg cgggcgaacg tgggatctcc ccagagcgtc
    10441 acgcgcaatt gctggctgaa ctcggcttag ataaaccgat gtggcagcag tatctccatt
    10501 acatttgggg cgtaatgcac ggtgatttag gcatttccat gaaaagccga attccggtat
    10561 gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc gcgatgattt
    10621 ttgcgaccgc agtcgggatt ccggttggtg tgctggctgc ggttaaacgt ggttccattt
    10681 tcgatcacac agcggttggc ctggcgctga ccggctactc gatgccaatt ttctggtggg
    10741 gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcctgtc tccggtcgtg
    10801 tgagcgacat ggtgttcctc gatgattcca atccgttaac cggttttatg ctgatcgaca
    10861 ccgccatctg gggtgaagac ggtaacttta tcgatgccgt cgcccatatg atcctgcccg
    10921 ccattgtgct gggtaccatc ccgctggcgg tcattgtgcg tatgacgcgc tcctcgatgc
    10981 tggaagtgct gggcgaagat tatatccgca ccgcgcgtgc caaaggtctt acccggatgc
    11041 gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtggtgacc gttatcggcc
    11101 tgcaggtggg aacattgctg gcgggggcga ttctgactga aaccatcttc tcgtggcccg
    11161 gtctggggcg ctggttgatt gacgcactgc aacgccgcga ttatccggta gtgcagggcg
    11221 gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat ctgctgtacg
    11281 gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca caggttactg
    11341 aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc tggcactatt
    11401 ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacgt cgtcatcgtg ctgtttatcg
    11461 cgatctttgc caactggatt gcaccctata acccagcgga acagttccgc gatgcactgc
    11521 tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc actgatgacg
    11581 tggggcgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg ctggttggct
    11641 gtctggtagt ggtgttatcg ctgattatgg gcgttattct cggcctgatc gctggttact
    11701 ttggcggtct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg gcgctgccaa
    11761 gtctactgct ggcactggtg ctggtggcga ttttcggccc gtcgattggt aacgccgcgc
    11821 tggcgctaac cttcgttgcg ctgccgcatt atgtgcgctt aacccgcgcc gccgtgctgg
    11881 tggaagttaa ccgcgattac gtcaccgcgt ctcgcgtggc gggtgccggg gcaatgcgcc
    11941 agatgtttat taacatcttc ccgaactgcc ttgcgccgct gattgttcag gcgtcgctcg
    12001 gtttctctaa cgccattctc gatatggctg ctcttggttt cctcggcatg ggcgcgcagc
    12061 cgccaacgcc ggagtggggc accatgctct ctgacgtgtt gcagttcgcg caaagcgcct
    12121 ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca tttaacctga
    12181 tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag gttcgagatg
    12241 gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc accgttccgc
    12301 gccgtagacc gcatcagcta cagcgtaaaa cagggtgaag tggtcgggat tgtgggtgag
    12361 tccggctctg gtaaatcggt cagttcgctg gcaattatgg ggctgattga ttatccgggc
    12421 cgcgtaatgg cggaaaagct ggagtttaac ggtcaggatt tgcagcgtat ctcagaaaaa
    12481 gagcgccgca acctggtggg tgccgaagtg gcaatgatct tccaggaccc gatgaccagc
    12541 cttaacccgt gctacaccgt gggtttccag attatggaag cgattaaggt gcatcagggc
    12601 ggcaacaaaa gtacccgccg tcagcgagcg attgacctgc tgaatctggt cggtattccc
    12661 gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat gagccagcgc
    12721 gtgatgatcg ccatggcgat tgcctgtcga ccaaaactgc tgattgccga tgaaccgacc
    12781 actgcgctgg acgtgaccat ccaggcgcaa atcatcgaac tgctgctgga gttgcagcag
    12841 aaagagaaca tggcgctggt gttgatcact catgacctgg cgctggtggc ggaagcggca
    12901 cataaaatca tcgtgatgta tgcaggccag gtagtagaaa cgggcgatgc gcacgctatc
    12961 ttccaggcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc ggaatttgct
    13021 caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta cgaccgcccg
    13081 aacggctgcc tgcttaaccc gcgctgcccc tacgccactg acaaatgccg tgctgaagaa
    13141 ccggcgctga atatgctcgc tgacgggcgt cagtccaaat gccattaccc acttgatgat
    13201 gccgggaggc cgacactatg agtacgcatg aggccacctc gcaacaaccg ctgttgcagg
    13261 ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg gaacgtctgg
    13321 tgaaggcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg gcagtagtag
    13381 gtgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt gaaacgccca
    13441 ccggcggcga actgtattac caggggcagg atctgctcaa gcacgatccg caggcgcaga
    13501 agctacgtcg gcagaaaatc cagattgtct tccagaaccc ttacggttcg ctgaatccgc
    13561 gtaaaaaagt tgggcagatt ctcgaagagc cgctgctgat caacaccagc ttaagcaaag
    13621 aacagcgtca ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa accgagcatt
    13681 acgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc attgcccgag
    13741 gtctgatgct cgacccggat gtggtgattg ccgatgagcc ggtttctgcg ctggacgtct
    13801 cagtgcgtgc gcaggtgctg aatctgatga tggatttgca gcaggaactg gggctatctt
    13861 atgtctttat ctcgcacgat ctgtcggtgg tggagcacat tgctgatgaa gtgatggtga
    13921 tgtacctggg ccgctgcgtg gagaagggga cgaaggacca aatcttcaat aacccgcgcc
    13981 acccgtacac tcaggcattg ctctccgcga caccacgcct gaacccggac gatcgtcgcg
    14041 aacgcatcaa acttaccggt gaactgccaa gcccgctgaa tccaccgcca ggctgcgcct
    14101 tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcatcca gttgcagccg cagctaaaag
    14161 actacggcgg tcaactggta gcctgttttg ccgttgatca ggatgaaaat ccgcagcgtt
    14221 aactcacaaa accggggcca tgcgctccgg tttactcaat caacttcaac agtggcgaca
    14281 agcataacaa aatgccgtac agcagaatca tccaggcttt aaacccgcga agtttttcca
    14341 gttgtgagac tttatagatc aggaaaaacg gaatgaggca cgacacaatg ccatataacg
    14401 ggcttcccaa ctgaaagaac accagtaccg atacacgaaa cgaaacccaa atcgtcaacg
    14461 taatgacgat aaaagtgcag atcgccagag tcagcacgag tgagttaatt ttcctggtat
    14521 caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg aaaccgagat
    14581 aaatgccaaa gaatgcagtc agtacggcaa agatattcag taccgtagag gtgatatgaa
    14641 tgatatgccc agggatcacc tgcgcggcca gtgccagtgc cgagatattt tgttcaaagg
    14701 cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa aacaagatca
    14761 ccgcaatgag cgtgatatag ctaatccggt gggtacgcag cgcgagccgg gttgccagta
    14821 ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc tgaataaata
    14881 ccgcggaaaa gaagcaaaat ggaatagtaa gcaggacatc gcggaaaaag tcggaggctt
    14941 gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga
    15001 caataatccc cactttgacc accaccattg gtccggaaat cttaaacagt aatcgttcac
    15061 caccagacgc aatcgccacc agtacggcga aaatggcgac tttataaaat agagattgag
    15121 aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg
    15181 agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa
    15241 ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta caggaatcgc
    15301 tttcagaaag ggtttttaaa taaatgtcct gtactaccca ggtggcagga taagcaatga
    15361 tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc atcagcacgg
    15421 ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa
    15481 acggcaattt ctgatcgtgt ttcggtagtg tgttgtgctg cataaagtcc tgtccagaaa
    15541 gaagtcggcc tcgcaaaaat gaataacccc agtttctctt gcgcaacagg tggagaagtc
    15601 gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc gaggaggcgc
    15661 aattctacaa aattgcgcat ttcactacta gtctggagtc agcccggata tgagctgcaa
    15721 ctattgcaaa taaagaaatt aagctggaag gcatcacgca atgaggaaga ttgccgggtg
    15781 ctgacggcaa aaaacaatcg taaaatcagc ggcaagccgg aaaggttccg gtgaggcgca
    15841 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
    15901 ttgcaggtat gcacctgaca atatcacggg ggctaatctt gaacctagac cactcaagaa
    15961 tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca gaactgggca
    16021 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
    16081 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
    16141 atttgcgtcc ggatcaggcc gcaggggtga aactctgcgg cctttttcgt tcttattgcg
    16201 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
    16261 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
    16321 tccagtcaac attgtcttcg gagaaaatct tgccatcaag aacgcgaacc accagatcgg
    16381 agatagccag gaagctgctc ggttggtcga tgacaatcgg agccccctga tgtggtgcct
    16441 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
    16501 gtagaccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
    16561 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
    16621 agaatttttg cgcccgcgct ttgtaatccg ctgttttgct gacgcccgga taatggttgc
    16681 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
    16741 taacgtccag ccaacggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
    16801 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc gctctgcatg ccgccatttt
    16861 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
    16921 taaagcccag tttcgacagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
    16981 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
    17041 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
    17101 gcgacatcaa tccggcggct tcaatatccg accaggaaag cgaacagata ttaatcacca
    17161 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaagatcgat ttacgtttcg
    17221 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt tgctgttgtt ggcggtgcag
    17281 tttgtgcggg tatatcaccc actaccggtg cgccacccgt cgccgcaacg gttgccgctg
    17341 cgttaccacc cgtcgttgtt acagtggtcg tcggttgacc ggctggccac aaggagaaac
    17401 ttggtcccgc cagggtaagt acgttcagcc atagcagaat ggcaaccaca aaaacggtaa
    17461 tgcgaatcca ttgtgacagg aataaccagg ccacgaataa aacaaaaatg gccccaatca
    17521 tctgccagtt aataaagcgt gtgacaagat cgattaaata atcggtactg aaccccgcca
    17581 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
    17641 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
    17701 tgggcatcag cagaaacgtg gcaaacacca gattgaggag cggatggaag ttaagatatc
    17761 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
    17821 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
    17881 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
    17941 aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag
    18001 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
    18061 gatgagcgct ccacagcatc atctaacagt cgcattggtt ctggtattcg tcgccagtgg
    18121 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgctaaagg cagcggcatg
    18181 ccccattgtt ctggggcaag caggcgcatc tgcaccagct cggcactgat ttgatcatct
    18241 tcaaaccaga ccattcggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
    18301 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
    18361 ccaatggtca tgatatcgcc ggtacggtta gggcgacata gcgtcagggc ttgctcaacg
    18421 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
    18481 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
    18541 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
    18601 aacttctgcc cttgcacgct ttcgatcatc gtcagacagc gggagagtgg cgcattccac
    18661 gggataacca tatttgcacc gcaggccaat aataaacgtt catcggtggc gcgcaggctg
    18721 gcggtatttt cccgtacgag gattttcatc gcactaccgc gctggcgacg cagagtatga
    18781 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
    18841 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
    18901 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
    18961 ctgcgtggtt ggatttccgc ctcctggtgt tgaactaatg tccagcaacc attttgttgc
    19021 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa agcaatatcc
    19081 agcaaatgtt ggtcgccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
    19141 tcaagtaaca atgaaaattg tttatcgtta ttatttccgg aattaattac caaaagcgaa
    19201 caatggttta gccggctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
    19261 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
    19321 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
    19381 aataatgtta tttttcccgg cccttgagaa tcatctaatt gaaagatttt cgctggatcg
    19441 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
    19501 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
    19561 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
    19621 tccctcatgt caacattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
    19681 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagca ggtcacaaga
    19741 catgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
    19801 tgagcacatt cccgtctcgc cgtaaacaat aaaccgacga aatgctcaca gttagaattg
    19861 tctcatgaac ggtacggtta tttcataggg atcaagaaaa atgaataaca atgaataaca
    19921 atgaaccaga tactctgcct gatcccgcga taggctatat cttccagaat gatattttgg
    19981 cgttaaagca ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac
    20041 agttggccgc ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg
    20101 ggattggtga a
//
LOCUS       DAZFRE010000087        19881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFRE010000087 DAZFRE010000000
VERSION     DAZFRE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.087"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            102..605
                     /gene="tssB"
                     /locus_tag="ACHKUI_003682"
     CDS             102..605
                     /gene="tssB"
                     /locus_tag="ACHKUI_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6599805.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
                     LGIAVQK"
     gene            628..2112
                     /gene="tssC"
                     /locus_tag="ACHKUI_003683"
     CDS             628..2112
                     /gene="tssC"
                     /locus_tag="ACHKUI_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6599806.1"
                     /translation="MSVKEEIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPVKTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2117..2542
                     /gene="tssE"
                     /locus_tag="ACHKUI_003684"
     CDS             2117..2542
                     /gene="tssE"
                     /locus_tag="ACHKUI_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6599807.1"
                     /translation="MLRRDSGPTGSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELCFHIVATVDVSETRGVFEFDILLDNHQRYCVE"
     gene            2548..4389
                     /gene="tssF"
                     /locus_tag="ACHKUI_003685"
     CDS             2548..4389
                     /gene="tssF"
                     /locus_tag="ACHKUI_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6599808.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSEKSSLTAQKALPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLEAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPINAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPL
                     RASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLLHHGLEHSIAFVHADGSIPGSSRFNDDVITTSLICTNRMIPARLHTGDI
                     CVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI"
     gene            4353..5402
                     /gene="tssG"
                     /locus_tag="ACHKUI_003686"
     CDS             4353..5402
                     /gene="tssG"
                     /locus_tag="ACHKUI_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6599809.1"
                     /translation="MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQD
                     ISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLSDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5419..6699
                     /gene="tagH"
                     /locus_tag="ACHKUI_003687"
     CDS             5419..6699
                     /gene="tagH"
                     /locus_tag="ACHKUI_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO6599810.1"
                     /translation="MAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDIGHTP
                     ECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPASSDALIRLRQGDEIQ
                     LGALALKVFLHDGAAVTYDERMATPETIVMNRDSLADTLLTTEGQPAYPGMPFQHQLA
                     PTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASDLTGANGINTPFMDLPPGN
                     ARHEDGDEFSAMAQYHLAVTPLLRGMECPLTLHNSQDADEFLEEAGRALQAAIKGLLS
                     LQQQQNSLSDKHLRPLEDNPLRLDMDYATALNVMFAEGKSPVHLAAPAAIAESLRNIR
                     HHEEANRAAIVEALRVMLDAFSPGNLMRRFAQYRRSHELRQKMDDAWAWQMYSNYYDE
                     LASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6696..7220
                     /gene="tssJ"
                     /locus_tag="ACHKUI_003688"
     CDS             6696..7220
                     /gene="tssJ"
                     /locus_tag="ACHKUI_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6599811.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFHAADYDQVATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7223..8569
                     /gene="tssK"
                     /locus_tag="ACHKUI_003689"
     CDS             7223..8569
                     /gene="tssK"
                     /locus_tag="ACHKUI_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6599812.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLTRRLDALGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRYQMQFTHRSQLHTLHPEAFYRDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATINDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPYHEGYVYFELEKGTPAWQDVIKAGALALHISGSFPDLNLQLWAIRG"
     gene            8574..9338
                     /gene="icmH"
                     /locus_tag="ACHKUI_003690"
     CDS             8574..9338
                     /gene="icmH"
                     /locus_tag="ACHKUI_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO6599813.1"
                     /translation="MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLG
                     MVMRLSTMNSQAMPEHLFTQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAAL
                     GNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
                     RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKH
                     VLCGGLVVLTVFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9348..12110
                     /gene="tssH"
                     /locus_tag="ACHKUI_003691"
                     /gene_synonym="clpV"
     CDS             9348..12110
                     /gene="tssH"
                     /locus_tag="ACHKUI_003691"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6599814.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFAAWTKESAESV
                     DLAGGQTPRATETGDTLLARYAKNMTADARNGRLDPVLCRNYEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVIFSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLEQYDETLSALDELEVAWQQQQTLVQEIIALRQQLLGVAEDDVA
                     SLPDADAVEDTPPEAEQDSTDAESADDAGSVQPEETAETVSPVQRLAQLTAELDALHN
                     DQLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGGTIKGQDLAIASL
                     HKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQ
                     EKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDK
                     GEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFKPALLAR
                     MEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGADVIIGPEVTDEIMSRVTRAENGA
                     RMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDALPDDAVTPQT
                     EDETVL"
     gene            12107..12850
                     /gene="vasI"
                     /locus_tag="ACHKUI_003692"
     CDS             12107..12850
                     /gene="vasI"
                     /locus_tag="ACHKUI_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO6599815.1"
                     /translation="MSRLCVFRQTGWFIAGLMAGLPASAAPAETSSVAGVAVAVATAT
                     PPDATATLQAMQSCRQETAALERLDCYDRILAPEQAGFGGAALVKARYQGEAWARATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12855..14297
                     /gene="tssA"
                     /locus_tag="ACHKUI_003693"
     CDS             12855..14297
                     /gene="tssA"
                     /locus_tag="ACHKUI_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6599816.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     ASELAKATDDLFSLFQRAFRDVAPEMTSSARSAAIAPQATTGYVPDMSPHTVAAQPPV
                     AATPIPQVTVESHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTDFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTVPVVQTD
                     EELQAAKACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFRTGLHMMLSEWEPSLLKALEQKLTAEQ"
     gene            14377..17844
                     /gene="tssM"
                     /locus_tag="ACHKUI_003694"
     CDS             14377..17844
                     /gene="tssM"
                     /locus_tag="ACHKUI_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO6599817.1"
                     /translation="MPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANR
                     WLATAVWGLIALVWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLR
                     LRRHLDNRRYLWQLPWYMVIGPAGSGKSTLLREGFPSDIVYTPESIRGVEYHPLITPR
                     VGNQAVIFDVDGVLTTPGGDDLLRRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLT
                     ADKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLNGFAALFHSLDKKDRDAI
                     LGVTFTRRAHESDGWRSELGAFWQTWVQQVNLALSDLVLAQTGAAPRSAVFSFSRQMQ
                     GTGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLAT
                     WPLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSTCGAALAALMVGSW
                     HHYYNQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLPLLNPVRDATLAYGDYRDHG
                     FLADMGLYQGARVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNIAPPESEEKLAVLRVV
                     RMMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDS
                     DAVSRWTPYDKPIINAQQELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNV
                     FVAGNDEKLVIPQFLTRYGLQSYFVKQREGLVELTALDSWVLNLTQSVAYSEADREEI
                     QRHITEQYLSDYTATWRAGMDNLNVRDYETMPALTDALEQIISGDQPFLRALTALRDN
                     THALTLSGKLDDKAKEAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQL
                     TELHRYLLAIQNSPVSGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLA
                     DQAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDG
                     ILDNFYKNNLRLFLENDLTFGDDGRMLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFA
                     VETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGRAP
                     RSIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYQVHVDTEDNPFTGGLFS
                     LFRLPDTLY"
     gene            17855..19202
                     /locus_tag="ACHKUI_003695"
                     /pseudo
     CDS             17855..19202
                     /locus_tag="ACHKUI_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            19220..19699
                     /locus_tag="ACHKUI_003696"
     CDS             19220..19699
                     /locus_tag="ACHKUI_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6599818.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLANARLSNIGLTIPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4628 a         5578 c         5523 g         4152 t
ORIGIN      
        1 agaaggaagg ttacattatc taactgccac tgaaacggta ttgtcggtat ttgtatgcag
       61 tacgaaaatg ctgtgctcat ggcctgaacg ggaacatttt tatgagcaaa atgaacaaca
      121 atggcggcag tgtcgcgccg aaagaaagaa tcagcgttcg gtatacacca aaagttgatg
      181 gggtggctgc tgatatcgaa ttgccgctga atttattgat caccggcaat ctgaaaggga
      241 aaccagataa cacgccgttg gatgagcgaa ctgccattgc tatcaaccgc tataacctca
      301 acgcagtcat ttcagaagct gacattgaac gggagtttgt tgttccggct gaactcagtg
      361 acgctcccaa tgagcaaatg tacgtcaacc tgaaagtcaa atctatggat gacctttctc
      421 ctgaccatat tgccagccag gtaccagaaa taaaacgtct gctcgaattg cgtgaagccc
      481 tggtcgcact taagtctcca ctaggcaata ttccagcctt ccgcgcacaa cttcaggcgc
      541 tactggaaaa cgaagacacc cgcgagcagc tgatccagga actgggtatc gccgttcaga
      601 aataattttc acagaaggat attcagtatg tcagtaaagg aagaaattgc tcccgtcagc
      661 gcgtctcaaa ccgccgcgcc cccatctctg cttgatgaaa tcatggcgca aacccgcgtc
      721 cagcctaaat cagaaagcta cgatatcacc cgccagggcg tgagcgcctt tattgccgcc
      781 atgttacagg gcgacagtag cgctgaacct atcaatatcc tggccgttga cgccatgatt
      841 gctgatattg atgcccgaac cagcaggcag atggatgcca ttattcatgc cccggagttt
      901 caggaactgg aatccctgct gcgctcgctg aagctgctgg ttgaacgcgc ggatacacgg
      961 gaaaacatca aagtccattt cctgaatgtc actcaggaag aactgctgga tgatttcgaa
     1021 tttgcacctg aaatcacgca gtctgcgtat tacaagcatg tatattcaag cggttacgga
     1081 caatttggcg gtgagccagt ggcggcggta atcggcaact ttgcctttaa gaacaccacg
     1141 ccggatatga agctgctgaa atatatcagc caggtcagcg cgatggcgca cagcccgttc
     1201 ctgtcgtcag tatcatccga atttttcggt ctggattcat ggacagagtt accggggatc
     1261 aaagaaccgg gtgccatctt tgaaggcccg gcctactccc gctggcgcgc cctgcgtgaa
     1321 tcggaagatt cccgttacct gggcctgacc gccccccggt tcctgctgcg ccacccttac
     1381 tcaccggatg aaaaccctgt caaaaccttc cgttaccatg aagatgtcag ccagagtcat
     1441 gagtcttatc tgtgggggaa tacctcgttt ttactggccg ccaacctggc ggaaagtttt
     1501 gctaagtatc gctggtgccc aaatattatt ggcccttcca gcggcggcgc agtcaaagac
     1561 ctcccggtgc atctgtatga atccatgggg caaatgcagg caaaaattcc gactgaagtg
     1621 ttgatcactg accggagaga gtatgagctg gcggaagagg gctttatcac actgaccatg
     1681 cgtaaagggt ctgataatgc gtgctttttc tcggccaact cggtgcagaa acccaaaaca
     1741 ttccccaaaa cccccgaagg caaggctgcg gagaccaact ataaactcgg cacccagctg
     1801 ccatacttgt ttgttatcag caggttagcg cattacatca aggtaattca gcgcgaacag
     1861 cttggctcat ggaaagaacg tagtgacctg gagcgtgagc tgaacacctg gattcgccag
     1921 tatgttgctg accaggaaaa tccgcctgcc gaagtccgca gccaccggcc tttgcgtcag
     1981 gctaagatcg aggtactgga tgtggatggt gagccgggct ggtaccaggt cgcgatttct
     2041 gtcagaccgc attttaaata catgggtgcc agtttcgacc tgagcctggt cggacgactg
     2101 gataaggaat aatccgatgc taagacggga ttcaggacca accggcagtc tttttgaacg
     2161 tatccgtgag gcggcaaatc cgccttcata tcagaatcca aaagaagcac tgatacgttc
     2221 catcagacgt aacctgcgac aggtcctgaa tacccgctcc ggcagttgtt atggttcacc
     2281 ggagctgggg attactgatt taaatgatga atcgctggcc tccagcgatt tcagacgaga
     2341 aatccgtaag tccattagcc agtgcatttt acattacgaa cctcgtatta ccgatgttgt
     2401 tgttacggct gctgcaccgg atgaatacgc ccctgtggaa ctgtgttttc atattgtggc
     2461 tacggtcgat gtcagcgaaa ccaggggagt gtttgaattt gacattttgc tggacaacca
     2521 tcaacgctat tgtgtggagt gatctctgtg gcttttgaag aacgttacta ccgcgaagag
     2581 ctggattatc ttcgccagct aggcaaactg ctggcgcagg aaaaaccaca tctggcccac
     2641 tttctggccg agaaagaggg tgatccggac gtggagcgtc tgatggaagc ctttgccttt
     2701 atgtccggta gcctgcgcca gaaacttgag gacgaattcc ccgagttcac ccatgggctg
     2761 atccgtatgc tgtgggcaaa ctatttacgc ccggttccgg ccatgacggt tattgcctat
     2821 gaacccaaaa ccgatcagtt aaaagtccct gttcaggtct gtcgtaatga actgatcaga
     2881 agccgctcag aaaagtcctc cctgactgct cagaaggcgc tgcctgacaa tcatgataaa
     2941 atcgtttctt ccgtagcctg ccatttcacg ctggcgcgcg atatctggct gcaaccactg
     3001 cgcattctcg atacccgcaa cgccagcagc ctgaaagaag gcatcataga tatcagtttt
     3061 accgcagata acaatgtctc ccccgccatg cttgacctga acaaaatcac gttctggctg
     3121 ggtaatgaag acgattatac ccgacaccag ctttatctgt ggttttgcga atgcctgatg
     3181 gatgcggaac tgattgccgg agagtaccgc ctgccgctgc cggatctgtg gctggaggcg
     3241 gcgggttttg acaaccggga tgctctgctg ccgtggccga aaaatgtcca cagcggctac
     3301 cgcgtgcttc aggagtattt ctgctatccc gaagccttct tctttttcca tctgcgcgat
     3361 gttgcgccgc taccagacga tttcccgatc aatgcgttta cgctgcgcct gcactttaac
     3421 cgcccgttac ctgccgacat caagctgcgc caggattcgc tgcgtctgca ctgtacgcca
     3481 gccattaacc tgtttacgca ttatgcggaa cccgtcaggc cggatgggcg gatggcggaa
     3541 tacccgctgc gcgccagcca taagcatccg gacgcctatg acatttttca ggtcagcaca
     3601 gtcaccagta aagtcaaagt ttcaggtact gaaatcagcc caggtagcca ggcgcgtgtc
     3661 tggcccgaat ttgaaagctt ccagcaccag atggagtaca gccgccagcg agaagtcgtc
     3721 tactggcatc accggactaa aacgtccctg cttcaccacg ggcttgaaca ctctatcgcc
     3781 tttgtccatg cagacggtag cattccgggc agctcacggt ttaacgatga cgtgatcacc
     3841 acgtcattaa tctgtaccaa ccgtatgatc cctgcccggc tgcataccgg tgatatctgc
     3901 gtggccgtca acaaaaatcc ggcagtggcc tcatttcgta atgtcacgcg cccgacactg
     3961 ccgctctggc cagtcacgga cggcgacatg cactggtcgc tgatttccgc gatgaacctg
     4021 aactacctct ccctgctgga cagggagaca ctgatccaga tcctgcggac cttcgacctg
     4081 ccaggtgctc accatccgca gcgggccaga ctgtcacgcc agaaactgga tgccatagaa
     4141 aaactcgaaa ccaaacctgt tgacaggttg tttaaaggcg taccggttcg cgggctggcg
     4201 accacgctgt ggatacgtcc cgatccgttt atctgcgaag gtgaaattta tcttctgggt
     4261 accgtgctct cgcacttctt tgccctctac gccagcatca attcgtacca ctgtctgaaa
     4321 atcattaaca cggaaagtca ggagtcctgg gaatggcagg agaaaatggg ccagcacgcc
     4381 ctgatatagc gacaaaaccg ccgttaccgg cggatgtgcg caactgcaat ttctatgtgc
     4441 tgatggaggc gctgtaccgt cggcatggcg cgccgggcca ggacatttcc ctgcgtaccg
     4501 agcctgcccg ggagatcgtg cgtttcagct ctgacgccag tatcagtttt ccgggtacgg
     4561 acctgagtgc gctttcccgt agccagaacg gacagtatgt actgcaaacc cggttcctgg
     4621 gtttttccgg cagccagtcg ccgctaccgg gctattatct ggatcagatg gcgcaggagt
     4681 ccgcacagaa cgaagacggc ctgaaagaat ttctcgatct attcagccac cgctggacac
     4741 agtttgccta tcatgcctgg cgcaaatacc gctactacat ctgctttcgt agcggcggta
     4801 ctgacacctt ttcacagcgc atgtacgcac tggtcgggct gggtaatcag agcgtgcgtg
     4861 acaggcttgc cattaaccac agcaaaatgc tggcctacgc cggaattctg gcaacaccag
     4921 gccgcgcgcc ggaggttatc tgtaatctgg tgtcgcactg ttttgatttg ccggatgtca
     4981 ccattgaacg ctggcaactg cgtaaagtcg ccattgatcc ggcacaccag aaccggctgg
     5041 gtgtacgtaa tccgaaagga aaaacggctg gacatattcc ggggcgctcc atactcggcg
     5101 ttaattttac cctcggcgca cgggtgccgg atcgcagcgg aaaattcctg ttgcagattg
     5161 gcggcctgtc gcgggagcgt tatctctcat ttttacccga tggcgaaaat cacctgccgc
     5221 tgaccatgtt cctttccttt attctgcgcg accagtttgc ctgggattta cggctgtgcc
     5281 tggcgccaca gcaggctaaa gggatgcgcc ttagcgatcc ggccagctcc cgcctcggca
     5341 gaaccagctt tattggtcag ccgaaagtac cgcccgccat cacaatccga atcagggaat
     5401 aattctatgg aaccacttat ggctgaggaa aaacagcaat cccgccaggc ctcactgact
     5461 ttacaggtga tgaatggcaa cgaactggaa agcggacgcg ccgcaaaatg cctgttcagc
     5521 gaaaacggcg gtgatattgg ccatacgccg gaatgtcact ggcaggtaca ggaccgggcg
     5581 ggaagcattg ctgcgcgtgc ctgtaccgtt atccggcacg acggagccta ttgcctgcgg
     5641 tgcctgacgc cgggactgat gattaatctg gccccggcct cttccgatgc gctcatccgc
     5701 ctgcgccagg gggatgagat ccagctcggc gcactggcgc tgaaagtgtt tctgcatgac
     5761 ggtgcagctg ttacctatga cgagcggatg gcgaccccgg aaacgattgt aatgaaccgc
     5821 gacagtctgg cggacaccct gctgaccacc gagggacaac cggcgtatcc gggaatgccg
     5881 ttccagcatc agcttgcccc cacggtggcg cacggttttt ccagcgatcc gctccaggca
     5941 ctccagacgg aaagcctgat ggtggcagat gatccgatcg ccccacgcgt ttcccggccc
     6001 gtagcccccg catccgatct gacaggtgca aacgggatca acacgccgtt tatggatctg
     6061 ccgcccggca atgcccgtca tgaggacggc gatgaatttt ctgccatggc gcagtaccac
     6121 ctggccgtaa ccccgctgct gcgcggtatg gagtgtccgc tgacgctgca caattctcag
     6181 gatgcggatg agtttctcga agaggccgga cgggcgttgc aggccgccat taagggattg
     6241 ctgagtctgc aacaacaaca gaacagcctg tcggataaac acctgcgtcc actagaagac
     6301 aacccgttgc gccttgatat ggactatgcc accgcgctga acgtcatgtt cgccgagggt
     6361 aaaagcccgg tacatctcgc agcacctgcg gccatcgccg aaagcctgcg caacatccgg
     6421 catcatgaag aggctaaccg ggcggcgatt gtggaagcgt tgcgtgtcat gctggacgcc
     6481 ttttcgccgg gcaacctgat gcgccgcttt gcgcaatacc gccgcagcca tgaactgcgc
     6541 cagaaaatgg acgacgcctg ggcatggcag atgtacagca actattacga tgagctggcc
     6601 tccagccgac agcagggatt tgaaatgctg ttcaatgaag tttatgccca ggtctatgac
     6661 cgggtgctgc gcgaaaaaca gcgggagccg gaagcatgaa cggcaaagcg tttctggcct
     6721 gcgttctgat gagcgtcgta ttaactggct gtgaaacagc gaaaaaaatc agccaggtga
     6781 tccgcaatcc ggatattcag gtcggaaagc tgatggatca gtcaaccgag ctgaccgtca
     6841 cgctgctgac cgagccggac agcaacctga cggcggatgg cgaagccgcg ccggtggatg
     6901 tccagttggt ttatctgagc gacgactcaa aattccatgc cgccgactac gaccaggttg
     6961 ccaccaccgc gctgcccgac gtgctgggga aaaactatat cgatcaccag gacttcaacc
     7021 tgttgccgga taccgtaaaa acactgccgc cgatcaagtt ggatgagaaa accggttata
     7081 tcggtgtcat tgcctatttt tcagacgacc aggccacaga atggaaacaa attgagtcgg
     7141 tagaaagtat cggccaccac tatcgcctgc tggtgcatat ccgcgccagt gcgattgaga
     7201 tgaaaaaaga ggaaaactga cgatggcaac aatgaaaaac aaagtgatct ggcaggaagg
     7261 gctgtttgcc ctgccgcagc attttcagca acagcagcgt cactgcgagt acctgctgac
     7321 ccgtcgtctg gatgcactgg gtgatttttt ctggggtttt acggaactct ccatcaatac
     7381 agaactgctt gcccagggca aaatcatgat tgaccgtgcg gcgggctgta tgcctgacgg
     7441 caccgtattc agcattcccg accaggatct gctgccggaa cctttccagc cgggaaccct
     7501 ttcatcgaaa gagagccaca atatttatct ggcgctgccg gtgataagcg acgtgatcaa
     7561 tgaaatccag gggttgcaca gcgccggtca gggtacggag cgttaccgcc tcacccatac
     7621 ccgcgtccgt gattttcaca ctgacgaagg cgacgaacag ccagtcggtc tggggcagtt
     7681 gatccccagg atagtcagcg gcgcagatga tctcagcgcg atggtgactc tccccctgtg
     7741 ccgtatcctc aataaaaatg ccaccggcgc gctggtgctg gacaacacct ttattcccac
     7801 cattcaggcc gtccgtgtca gcgggttgct cggggcattc tccggcgagg tgcagggact
     7861 gcttgccacc cgcgccgctg acctggccgg gcgcattggt tcgcctgaac agagcgggat
     7921 cgccgatgtg gccgaattta tgatgttgca gatgcttaac cgctatcaaa tgcagttcac
     7981 gcatcgttcc caactgcaca cgctgcatcc ggaagccttc taccgggatc tggtggggct
     8041 gctgggagaa ctgatgacct ttaccgaagg taatcgcctg ccctgcaccg tggagccgta
     8101 taatcaccgg gatcttaccg cgaccttcac cagaaccgtg atcccggaac tgcggcgcgc
     8161 gctcaatacc gtactggtgc cgcgtgcgca gaatctgccg ttgttctttt cagaggggac
     8221 ctggatcgcc accatcaacg atcccacatt gttgcagtcc tgcaagctgg tactggcagt
     8281 gcgcgccagg atgccctacg accaggtaca acgccagttc atccagcagt cgaaagtggc
     8341 cgctaccgac aaaatccgca gcgtggtcag cgtgcaggtg ccgggtattc cgctgcacac
     8401 gcttaccgct gcgccacgtc agttgccata ccacgaaggt tatgtttact tcgaactgga
     8461 aaaaggcacc ccggcctggc aggacgtgat aaaagcgggc gcactggcgc tgcatatctc
     8521 cggcagcttc cccgatctga acctccagct ctgggctata aggggataag tgaatgagcg
     8581 atatgagtga agcgtcggtt tttcccgtgg agccggagag cggaacacca ggacgccagt
     8641 accgtctcgc ccttcgcggc aacagcctga atccaatgat tgacgccgcc acgccgttgc
     8701 tcggcatggt gatgcgtctg tccactatga acagtcaggc catgccggaa catctgttta
     8761 cccaggtagt aaccgatgtc caggccgtag agcaactact ccaggaacag ggatacgaac
     8821 cgggcgtcat tgtttcgttc cgttacattc tctgcacgtt tattgatgaa gcggcgctgg
     8881 gcaacggctg gtccaacaaa aacgaatgga tcaaacagtc gctgctggtt cattttcata
     8941 acgaagcctg gggcggcgag aaagtgttta tcctgctcga acgcctgatc cgggagccgg
     9001 tacgttacca ggatctgctg gaatttttat acctctgttt ttcgctcggc tttcgcgggc
     9061 gctacaaggt tgccattcag caacaggatg aatttgagcg gatctaccag cgcctgcacc
     9121 atgccctgca caaactgcgc ggtgacgcgc catttccgct acttcatcag aagaacaaca
     9181 tacagggtgg gcgttatcag ttaatccgac gactgaccat caagcatgta ctgtgcggcg
     9241 gtcttgttgt gctgaccgta ttttatctgt tttatctgct gcgtctggac agccagacac
     9301 aggatattct gcatcagctc aataaattac tccgataagg acatcttatg atccagattg
     9361 atttagccac gctcgtaaag cggcttaacc cctttgcaaa acaggcgctg gaaatggctg
     9421 cctcggagtg tatgagccag caggccagtg agatcaccgt tgcccatgtg ctgttacaga
     9481 tgctggcaat cccgcgcaac gacgtaaggg tgattgccga acgcacaggc atcagtgcgg
     9541 aggatttacg ccaggcgctg accgtggaga gttatcccgg tggacgttcc gccgaaggct
     9601 atcccagctt ctccccgatg ctgatcgaat ggctcaagga gtcatggctg ctggcctcag
     9661 cccagatgca gcacagcgaa ctgcgcagcg gcgtcctgct gcttactctg ctgcattcac
     9721 cgctgcgcta tattccaccc gctgctgccc gtctgctgac ggccataaac cgcgaccagt
     9781 tacagcagga tttcgcagca tggacaaaag aatccgccga atccgtggat ctggctggcg
     9841 ggcaaacgcc ccgcgcaacg gaaaccggcg acaccctgct tgcccgctac gccaaaaaca
     9901 tgaccgcaga cgcccgtaac ggcaggcttg acccggtact gtgccgcaac tatgaaatcg
     9961 acctgatgat cgacattctc tgccgccgcc gtaaaaacaa tccggtggta gtgggcgaag
    10021 caggcgtggg taaaagcgcg ctgattgaag ggctggcgct gcgcatcgtg gcaggccagg
    10081 tgccggacaa gctgaaaaac accgatatca tgacccttga cctgggcgca ttgcaggccg
    10141 gtgcgtcggt gaaaggtgag tttgaaaaac gcttcaaggg gctgatggcg gaggtcattt
    10201 tctccccggt gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca
    10261 accagcaggg cgggctggat atatccaacc tgctcaaacc agcgctggcg cgcggcgagc
    10321 tgaaaaccat cgccgccacc acctggagcg agtacaaaaa atatttcgag aaagatgcag
    10381 ctttgtcgcg ccgcttccag ttagtgaagg tcagcgaacc caacgctgcc gaagccacca
    10441 ttattctgcg cggtttgtcg gcggtctatg aacaatctca cggcgtgctg attgatgatg
    10501 acgccttgca ggccgctgcg acactgagcg agcgttatct ctccgggcgt cagttgccgg
    10561 acaaagccat tgatgtgctg gacaccgcct gcgcccgtgt ggccatcaac ctgtcatcgc
    10621 cgccaaaaca aatctcggca ctgaccaccc tgagccacca gcaggaggca gaaattcgcc
    10681 agcttgagcg cgagcttcgc atcgggctgc gtaccgacac ctcacgaatg actgaggtgt
    10741 tggagcagta tgatgaaacg ctgtcggcgc tggacgaact ggaagtcgcc tggcagcagc
    10801 agcagacgct ggtacaggaa attatcgcgc tgcgccagca gttactgggc gtggcagaag
    10861 acgacgttgc gtcgttgccg gacgcagatg ccgtggagga tacgccgcca gaggcagaac
    10921 aggatagtac cgatgccgaa tcggctgatg atgcaggcag cgtacagccg gaagagaccg
    10981 ctgaaacagt ttccccggta caacggctgg cacaactcac tgccgaactg gacgccctgc
    11041 ataacgacca gttgctggtt tccccgcatg tcgataaaaa acagattgca gcggtgattg
    11101 ctgaatggac cggcgtgccg cttaaccgcc tgtcacagaa tgagatgtcg gtcatcaccg
    11161 acctgccggt atggctcggc ggcaccatca agggccagga cctggcgatt gccagcctgc
    11221 ataaacacct gctgaccgca cgcgccgacc tgcgccgtcc gggacgcccg cttggtgcgt
    11281 tcctgctggc tggccccagc ggcgtgggta aaaccgaaac cgtcctgcaa ctggcggaac
    11341 tgctctatgg cggacgtcaa tacctgacca ccatcaatat gtctgagttc caggagaaac
    11401 acaccgtttc gcggctgatt ggctctcctc cgggttatgt cggctacggt gaaggcggcg
    11461 tactgaccga agcgattcgc cagaaaccgt actcggtggt gctgctcgat gaagtagaaa
    11521 aagcgcaccc ggatgtactc aacctgttct accaggcgtt tgacaagggc gagatggcag
    11581 acggtgaagg ccgcctgatt gactgtaaaa atatcgtttt cttcctgacc tccaaccttg
    11641 gctaccaggt gattgtcgaa cacgccgatg acccggaaac catgcaggaa gccctctatc
    11701 cggtgctggc ggacttcttt aaacctgctc tgctggcgcg catggaagtg gtgccttacc
    11761 taccgctgtc gaaagagacg ctcgctacca ttattgccgg gaaactggcc cgcctggata
    11821 acgtgctgcg cagccgcttt ggcgcggacg tgattattgg gccggaagtg acggacgaaa
    11881 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gttatcgacg
    11941 gcgacatgct gccgccgctc tcgctgctgc tgttgcagaa aatggcggcc aacacggcga
    12001 ttgcccggat tcgcctgtcg gcggcagacg gtgcgttcac ggcagatgtg gaagatgcgc
    12061 tgcctgatga tgccgtgaca ccgcaaacag aggatgaaac ggttttatga gcaggttgtg
    12121 cgttttccgg cagacaggat ggtttatcgc gggcctgatg gcgggcttac ccgccagtgc
    12181 cgcgcccgca gaaacatcgt cagtggccgg tgtggcggta gctgtcgcca cagcgacacc
    12241 gccggatgcg acagccacgt tacaggccat gcagtcctgc cgccaggaga cggcggcgct
    12301 ggagcggctt gactgttatg accgcattct tgcgccggaa caggctggct ttggtggtgc
    12361 cgcactggtg aaagctcgtt accagggtga agcctgggcc cgcgcgacag aacaggaaaa
    12421 acgccgccag ggcaacacca cggagctgct ggtaacgcaa gtaccgggtg aacgcccaac
    12481 ggtggtgata accaccccgg ctatcggcca tgttccgcca cgcccggtgc tgatgttcag
    12541 ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg caccctctgg atgtccatga
    12601 tatcgccgtc accctgaatg ccgacagccg ggcattgcgc agccactggt ttgtgcgcga
    12661 gaacggcacc ctgctggagt ccagccgggg gttatccggt atcgatgaaa ttaagcaact
    12721 gttcggcgca aaaacgctga cggtggatac cggcacagac aacgccgccg gaaagctgac
    12781 ctttaacatt gacgggctgg cacgggttat tgcgccgcta cgcgacgcct gccactgggc
    12841 gggagaataa gaatatggca atggacttac gtaatcccga tgtatggctt gcacacctgc
    12901 tggaaaacct gccagaagat aagctgtccg ccgcgcttga tgatggcaat gcagactggg
    12961 agtttgtcga cagcgaaatc gtcaagctcg gttcactggc gcacagccag cttgatatcc
    13021 cggaacttca gcgccggggc ctgatgcttc tggcctcaga aaccaaagat tttcgcctgc
    13081 tggcccactt gctgcgtacc ctgcaacatg ccggtgatat tctgctggcc tccaggatgc
    13141 tggcacaata tacagagcac tactggacat gcgccgcacc gcagaacatg gcccacaaaa
    13201 aacgctttgc cgcccaggtg ataaaacgct ttgagtctgc cgttcaggac tttgccggga
    13261 atgctgcgac aatacagcgc gatgctttgc tgggagagct ggcaaaactg gcacagtgct
    13321 ggcaggcgca caacgcgtct gagctggcaa aggcgacaga cgatttattt tccctgtttc
    13381 agcgcgcgtt tcgtgatgta gcgcctgaaa tgacatcatc tgcacgaagc gccgccattg
    13441 cgccgcaggc cacgaccgga tatgtaccgg atatgtcacc gcacacggtt gccgctcagc
    13501 ctcctgttgc agccactcca atcccgcagg tcaccgttga aagccacgac gataaagcat
    13561 ggcgcgacac cctgctaaag gtggccgcca ttctctgtga gcgccagccg gagtcaccgc
    13621 agggttaccg actgcgccgc catgcgctgt ggcaaaccat caccagtacc ccgcaggcag
    13681 aaagcgatgg ccggacccca ctggcagcct tccctgtcga tatgatggat gattacctgg
    13741 cgcggctgaa caatgccgac atggcgctgt ggcagcaggt ggaaaaaagc ctgctgctgg
    13801 ccccgtactg gctggacggt cattacctct cagcacagac cgcgctgcgc ctcggttata
    13861 aacaggtggc ggatgctatc cgtgatgaag ttaccgattt tctggcccgc ctgcccgcac
    13921 tgatcaacct gttgtttaac gaccgcactc cgtttgtgtc cgaacagacg aaacagtggc
    13981 tggcatcatc cggctcagtc aaccagaccg tcccggttgt acagaccgat gaagaattac
    14041 aggcggccaa ggcgtgcttc gatgaaaacg ggctggaagc cgcgctgcgg tacctggaaa
    14101 acctgcccga aggtgatccc cgccaccagt ttcaccgcca gtttttcggc gcgcagttac
    14161 tggaagaggc cggtatggtt cagcttgcac aacagcagta ccggatgctc ttcagaacag
    14221 gattacacat gatgctatcc gaatgggagc cttccctgct gaaagcactg gaacaaaaac
    14281 tcacggcaga acagtaaaag ataacaagga gttactgtgt tcaaatttcc cacatcccga
    14341 ctgttcagca cgttgaaatc tgcgctcagg ccagcgatgc cgcggtttaa agtttccgcc
    14401 acctggctac tgacgctggc atggattttt ctgctggtgt ggatctggtg gcagggtcca
    14461 aaatggacgc tctatgagca gcactggctg gctccgctgg caaaccgctg gctggcgacc
    14521 gccgtctggg gacttatcgc tctggtctgg ctcacctggc gggtgatgaa gcgtctgcaa
    14581 aagctggaaa aacagcagaa acagcagcgg gaggaagaaa aagatccgtt gaccgtggaa
    14641 ctccaccgcc agcagcaata tctggatcac tggctgctgc gcctgcgccg ccatctggat
    14701 aaccgccgtt atctgtggca gttgccgtgg tatatggtca ttggtcctgc gggtagcggc
    14761 aaaagcacgc tgctgcgcga gggctttccg tctgacattg tttacacgcc ggaaagcatc
    14821 cggggtgtgg aataccaccc gctgatcaca ccgcgagtgg gcaaccaggc ggtaattttc
    14881 gatgttgacg gcgtactgac cactcccggc ggggatgatc tgctccgccg ccgcctgcgc
    14941 gaacactggc tgggctggct gatgcaaacg cgcgctcgcc agccgctcaa cggtcttatc
    15001 ctgacgctcg atcttcccga tctgctgacg gcggataaat cccgccgtga gacactggta
    15061 caaaatttgc gccagcaact tcaggagatc cgtcagagcc tgcactgccg tctgcccgtt
    15121 tacgtggtgc tgacacggct ggatctgctg aacggctttg ccgcgctgtt ccattcactg
    15181 gataaaaaag accgcgatgc gatcctcggc gtcacattta cccgccgcgc ccatgaaagt
    15241 gacggctggc gcagcgaact gggggctttc tggcagacgt gggtacaaca ggtgaacctg
    15301 gcgctgtcgg atctggtgct cgcacaaacc ggtgctgctc cccgcagcgc tgtgttcagc
    15361 ttctcccgtc agatgcaggg aacaggagaa atcgtcaccg cactgctcgc cgcattgctg
    15421 gacggtgaga acatggatgt aatgctgcgt ggcgtctggc tcacatcctc gctacagcgt
    15481 ggccaggtgg atgatatttt cacgcagtcc gccgcccgcc agtacggact gggtaacagc
    15541 tcgctggcaa cctggcctct ggtggagacg acgccgtatt ttactcgccg cctcttcccg
    15601 gaagtcctgc tggctgagcc gaacctggcg ggtgaaaaca gcgtctggct gaacagctcc
    15661 cggcgcaggc tgaccgcctt ttccacctgt ggcgcggcac tggcggcatt gatggtcgga
    15721 agctggcacc attattacaa tcagaactgg cagtctggcg ttaacgtact ggcacaagct
    15781 aaagccttta tggacgtacc accaccgcag ggaacggatg aattcggcaa tctgcaattg
    15841 ccattgctta acccggtacg cgatgccacc ctggcctatg gtgattatcg cgatcacggt
    15901 tttctggcgg atatgggatt gtaccagggc gcccgcgtag ggccgtatgt ggagcaaacc
    15961 tacattcagc ttcttgagca gcgttatctc ccctcgttaa tgaacggcct gatccgggat
    16021 ctaaacattg ccccgccaga gagcgaagaa aagctcgctg tgctgcgcgt agtgcgcatg
    16081 atggaagaca aaagtgggcg caacaacgag gcggtaaaac agtacatggc gcggcgctgg
    16141 agcaatgaat ttcacggcca gcgcgatatt caggcgcaac tgatggcgca tctggactat
    16201 gcgctggagc acaccgactg gcacgcgcag cgccagagcg gtgacagcga tgctgtcagc
    16261 cgctggaccc cctatgataa accgatcatt aatgcgcagc aggaactgag caagctgccc
    16321 atataccagc gtgtctacca gaccctgcgc accaaagcat taagcgtgtt gcccgccgat
    16381 ttgaatttgc gcgaccaggt tggtcccacc ttcgacaacg tgttcgtcgc cggtaatgat
    16441 gaaaaactgg tgatcccgca gttcctcacc cgctatggac tgcaaagcta ttttgtcaaa
    16501 cagcgtgagg gcctcgttga gctgaccgcg ctggattcgt gggtactgaa cctgacgcaa
    16561 agcgtcgcct acagcgaggc cgaccgtgaa gagatccagc gccatatcac tgaacagtac
    16621 ctcagtgact ataccgctac atggcgtgcc ggaatggata atctcaacgt ccgtgactat
    16681 gagaccatgc cggcgctgac cgacgcgctg gagcagatta tcagcggtga tcagccattc
    16741 ctgcgtgctc tgacggcgct gcgcgataat acacacgcgc tgacgctctc cggcaaactg
    16801 gacgataagg cgaaggaagc ggcgataaat gagatggatt accgcctgtt atcccggctg
    16861 gggcatgagt tcgcgccgga aaatagcgca ctggaggagc aaaaggacaa ggcgagtact
    16921 ctacaggccg tgtatcagca actgaccgag ctgcaccgtt acctgctggc gatccagaac
    16981 tcgccagtgt ctgggaaatc ggcgctgaaa gcagtacagc tacggctgga tcaaaacagc
    17041 agcgatccaa tcttcgccac ccgtcagatg gcaaaaaccc tgcctgcgcc tcttaaccgc
    17101 tgggtaggta agctggcgga tcaggcctgg catgtagtga tggtggaagc cgttcgttac
    17161 atggaagtgg actggcgcga caatgtagtg aaacccttca acgagcagct tgccgataac
    17221 tatccgttta atccgcgcgc cacacaggat gcctcactgg attcgtttga acgtttcttt
    17281 aaaccggatg gcattctgga caatttctac aagaacaacc tgcgcctgtt ccttgaaaac
    17341 gatctgacct ttggcgacga cggcagaatg ttaatccgtg aagatatccg gcagcaactg
    17401 gatactgcgc agaaaatccg cgacatcttc ttcagccagc agaacgggct gggcgcacag
    17461 tttgccgtgg aaaccgtatc gctttccggc aataagcggc gcagcgtact taacctggac
    17521 ggccagttag tggactacag ccagggacgc aactacaccg cccatctggt ctggccgaac
    17581 aacatgcgtg aaggcaatga aagcaagctg acgctgattg gcaccagcgg cagagcaccg
    17641 cgcagtatcg cgttcagtgg accgtgggcg cagttccgcc tgttcggcgc gggccagttg
    17701 accaatgtga ccagtgacac ctttaacgtg cgctttaacg tggacggcgg cgcaatggtt
    17761 taccaggtgc atgtggatac cgaagataac ccgttcaccg gcggtctgtt cagcctgttc
    17821 cgtttaccgg atacgttgta ttaaggggat agtgatgagc aattacgcac tgacacaaac
    17881 tatcgttacc ggcagcgatc cgcgcgccct gccggagttc agcgccatcc gcgaagaaat
    17941 taacaaagct aaccacccgt cacagccgga gctgaactgg aaactggtgg aatcgctggc
    18001 gctgtcgatt tttaaggcca acggggtgga tttgcatacc gccacctact atacgctggc
    18061 acgtacacgt aatcagggac tggcagggtt ctgtgaaggc gcggaactgc tggctgcgat
    18121 gatcatccac gaatgggata aattctggcc gcagagtggt ccggcgcgga ctgagatgct
    18181 ggactggttc aacacccgca ccgggaatat cctgcgccag caggtttcct tttccgaaaa
    18241 tgatctttcg ttgctctacc gcacggaacg ggctttgcag cttatctgcg acaaactcca
    18301 gcaagtggaa ctgaagcgcc agccgcgcgt ggaaaatctg ctttattttg tgcagaacac
    18361 acgcaagcgg tttgagccgc agcccagaaa ccgtactgat acggcggcac agaccatggt
    18421 cagaacgctg gtctatgcgc ctgaaggcac ggcttccgcc acagcggaaa ccatgccccc
    18481 cctacccgac ctgccggaaa tgcgggtggg ggtgcgcggt gtggcagata acgccgacaa
    18541 agcaaaacag ggcgacacgg taaaaggttt tgttgccggt gccgcctgta ctgccgttat
    18601 cgcatctgca ctctggtggt ggcaggcgta tccgatgcag cagcagctta cacaggtcag
    18661 ggataccaca cagggagcca ccaccgtctg gttggcatcc ccggtactga aagaatatga
    18721 acagtacctg caacagcttc tgaatgcccc gccactacag ccgctggaaa ccggaatgca
    18781 gatgatgcgc accgcagaca ccctctggcc ggaaagcctg caacagcagg atgtggagca
    18841 acacgctgag aaaccgggcg caagccagtc cgcagatgaa gggctggcaa caagcgcggc
    18901 agaatttgcg ggattttgca gatttgatga tgaagaagga gacggagaaa cagggtttca
    18961 ccctatcgta tatcaaaacg gtgacctggc aggccgaacg gttactgaat caagaaacac
    19021 cactggagta cctgctgacg cagtaccagg agacccgtac acagaaacag gacacacagg
    19081 cgttggaaaa agagatcaac gaacggctgg acgggctgtt aagccgctgg ctgctgctga
    19141 aaaataccgg acaggatatg gcaacggata acagaaccga acctgtccat ccaactcatt
    19201 gagattaagg agaataacaa tggcaaatcc cgtctatctg acactgaacg gagaacttca
    19261 ggggctgatt tcatccggat gctcgtcaat gccgtccatc ggtaacaaag cccagattgc
    19321 acatcaggat cagatcatgg tgacgtcact gtcccatggt ctgagccgtg cgcagaatgt
    19381 caaccatcag gagttgacca tcacgaaacc ggtggacaaa tcctcccctc tgctgggcaa
    19441 ggccatttca gagaatgaat gtctgacctg tgattttgta ttctaccgca ccaaccgctt
    19501 tggaattaat gaaccgtact acaagctgaa actggctaac gcccggctct caaacatcgg
    19561 ccttaccatt ccccatacca ttaacgacag tcctgggcag gcggaagagt ctgtgtcatt
    19621 tacctatgag agcatcaatt gggagcacag cgtcgccgga accagtgcat acagcctttg
    19681 gagtgaacgg attttctaag ttaccaggga gtctttcatc tgcattattg ttatatttta
    19741 aaaaggcaat ttaacattat aggataaaat gacaaagtac ggttaatcac gaataaaaat
    19801 tcttaattaa ccgtacctgc tgttttatag atgtatatga tgtatttttg acagttcaac
    19861 cgaaaactcg aattacagct t
//
LOCUS       DAZFRE010000088        19860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFRE010000088 DAZFRE010000000
VERSION     DAZFRE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.088"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..324)
                     /locus_tag="ACHKUI_003697"
     CDS             complement(<1..324)
                     /locus_tag="ACHKUI_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO6599819.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDK"
     gene            complement(417..608)
                     /locus_tag="ACHKUI_003698"
     CDS             complement(417..608)
                     /locus_tag="ACHKUI_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6599820.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(605..793)
                     /gene="dicB"
                     /locus_tag="ACHKUI_003699"
     CDS             complement(605..793)
                     /gene="dicB"
                     /locus_tag="ACHKUI_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6599821.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCAQ"
     gene            1356..1616
                     /locus_tag="ACHKUI_003700"
     CDS             1356..1616
                     /locus_tag="ACHKUI_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599822.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHEKEHVKSVINSLILNQKN"
     gene            complement(1594..1914)
                     /locus_tag="ACHKUI_003701"
     CDS             complement(1594..1914)
                     /locus_tag="ACHKUI_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599823.1"
                     /translation="MEFKDLPMPFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKTKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(1937..2156)
                     /gene="ydfC"
                     /locus_tag="ACHKUI_003702"
                     /pseudo
     CDS             complement(1937..2156)
                     /gene="ydfC"
                     /locus_tag="ACHKUI_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
     gene            complement(2186..2314)
                     /gene="ydfB"
                     /locus_tag="ACHKUI_003703"
     CDS             complement(2186..2314)
                     /gene="ydfB"
                     /locus_tag="ACHKUI_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6599824.1"
                     /translation="MDFDTIMEKAYEEYFDGLAEGEEALSFSEFKQALSSSGKSNG"
     gene            complement(2316..2471)
                     /locus_tag="ACHKUI_003704"
     CDS             complement(2316..2471)
                     /locus_tag="ACHKUI_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6599825.1"
                     /translation="MDTIELGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2725..3186)
                     /locus_tag="ACHKUI_003705"
     CDS             complement(2725..3186)
                     /locus_tag="ACHKUI_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599826.1"
                     /translation="MKKTREVIATPEASKNLKAAWNARKKELKLTQELAAELLGFESQ
                     GTVSQYLNGKIPVNTDAALKFAALLKVKPEDIREDLKDLMNYVRSSDTYDDNFSGKGW
                     RLVNEEQAELLNLFEILPASEKAKLLNQLRGLNKLYEEAFENMLALKKRNQ"
     gene            3294..3569
                     /locus_tag="ACHKUI_003706"
     CDS             3294..3569
                     /locus_tag="ACHKUI_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO6599827.1"
                     /translation="MNLRDYLKEKHITQLQFGKLTGLSQVHVSRVLGGYERFSPEKAL
                     RVAEVTNFEVTPHELRPDIYPNPTDGLPVGFKANTPNASELIHENQA"
     gene            3553..3978
                     /locus_tag="ACHKUI_003707"
     CDS             3553..3978
                     /locus_tag="ACHKUI_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6599828.1"
                     /translation="MKIRHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            4050..5090
                     /locus_tag="ACHKUI_003708"
     CDS             4050..5090
                     /locus_tag="ACHKUI_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO6599829.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEEVIDSILQAMQGRVLDGDLITGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKQNEGCHDESRICHDMSRRVTTDKDTDKE
                     LNPTHNAHVRESAPTSESSGTPLQAAEPASLDGLSEPIGKFPMVDDWHPSPDFRRRAA
                     LWGMALPEPEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            5083..5544
                     /locus_tag="ACHKUI_003709"
     CDS             5083..5544
                     /locus_tag="ACHKUI_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6599830.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRDESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            5578..6348
                     /locus_tag="ACHKUI_003710"
     CDS             5578..6348
                     /locus_tag="ACHKUI_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6599831.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALREVNKHRDIVRQITDSSRRVVSEK"
     gene            6364..6756
                     /locus_tag="ACHKUI_003711"
     CDS             6364..6756
                     /locus_tag="ACHKUI_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6599832.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            6753..7049
                     /locus_tag="ACHKUI_003712"
     CDS             6753..7049
                     /locus_tag="ACHKUI_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6599833.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            7046..7507
                     /locus_tag="ACHKUI_003713"
     CDS             7046..7507
                     /locus_tag="ACHKUI_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6599834.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            7485..7784
                     /locus_tag="ACHKUI_003714"
     CDS             7485..7784
                     /locus_tag="ACHKUI_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599835.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLI"
     gene            7937..8119
                     /locus_tag="ACHKUI_003715"
     CDS             7937..8119
                     /locus_tag="ACHKUI_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599836.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIQDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            8112..8288
                     /locus_tag="ACHKUI_003716"
     CDS             8112..8288
                     /locus_tag="ACHKUI_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599837.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            8285..8644
                     /locus_tag="ACHKUI_003717"
     CDS             8285..8644
                     /locus_tag="ACHKUI_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599838.1"
                     /translation="MSEINYQEGHEKVGQAKPVAWRYRYVKKGVTDFQGKQWVGDWKY
                     VPTKEDCNDRPNYEIQALFTAPPASVTSEGLVKAVRFYEQVRREDPPVETGAWKDAID
                     WVLKEACLVVNTGIKGG"
     gene            8645..8860
                     /locus_tag="ACHKUI_003718"
     CDS             8645..8860
                     /locus_tag="ACHKUI_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599839.1"
                     /translation="MGIAASYTMHLYCDCRQCTDGKYQSPDFGEYIGTSWAGCAKEAR
                     KDGWRISADKTRAFAPGHKILRSNKGE"
     gene            8862..9080
                     /locus_tag="ACHKUI_003719"
     CDS             8862..9080
                     /locus_tag="ACHKUI_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6599840.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMIPISPLILVWGIGKII
                     EPVIELYNDVVWAPFNTLHNKINPYKEN"
     gene            9082..9345
                     /locus_tag="ACHKUI_003720"
     CDS             9082..9345
                     /locus_tag="ACHKUI_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599841.1"
                     /translation="MATLTKKEQAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            9356..9523
                     /locus_tag="ACHKUI_003721"
     CDS             9356..9523
                     /locus_tag="ACHKUI_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599842.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            9520..9864
                     /locus_tag="ACHKUI_003722"
     CDS             9520..9864
                     /locus_tag="ACHKUI_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599843.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            10156..10311
                     /gene="hokD"
                     /locus_tag="ACHKUI_003723"
     CDS             10156..10311
                     /gene="hokD"
                     /locus_tag="ACHKUI_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6599844.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            10477..11127
                     /locus_tag="ACHKUI_003724"
     CDS             10477..11127
                     /locus_tag="ACHKUI_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599845.1"
                     /translation="MNENQLAPCWEFQPYLAENYVRHLLAEIANVLEQLYYHKHALDS
                     NWSEGVRAYDWVRNHLIQNEDAIPGLEMISKGLDYVVALNKVPLQFTKDCINNPKKKH
                     RLRRNKVEYEQLSLFGDVEAEQDITWRVIAEPFLSEEGDGELESTLPRWEVALVGFNT
                     YGAQISIVSHQSTASMPLMPLDYNTLPDEAEINKVPLRRRTKDKDLDVSSDGTSGE"
     gene            11108..12211
                     /locus_tag="ACHKUI_003725"
     CDS             11108..12211
                     /locus_tag="ACHKUI_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599846.1"
                     /translation="MEHQVNNFTEYRGDKLKLARMAVGLSCEELAEKIGKTKQFVSKL
                     EKGCRPSEQCLELISSALMIKSSFLFTERKYALESDVCHFRSKKSRTQTLTNSVLARA
                     EILNIIISAVEGEIEFPDVNIPEHPGAELLTPNDIERVAEDCRRAWNLGLGPISSMVK
                     LAESLGVIVAHVTGVDDRVDAFTVHNNRPVIIRNNVKKSICRFRSDLGHELGHLVMHE
                     GITTGDKLTESQADHFSSALLVPRLSFIKEFPRIRGKQFDWNALVEFKLRWKISLKMC
                     IYRASALGLLTQEQARTGYMHLNSRGYTRVEPGDELLRPEEPGMLAEAIEMLDDATWL
                     RILMKTGLSQDLIRELFSINRPITNPRNIFQIV"
     gene            12369..12443
                     /locus_tag="ACHKUI_003726"
                     /pseudo
     CDS             12369..12443
                     /locus_tag="ACHKUI_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12602..12874
                     /locus_tag="ACHKUI_003727"
     CDS             12602..12874
                     /locus_tag="ACHKUI_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599847.1"
                     /translation="MAHNFKFRNSDSAFASRHGWRSHAFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFRKALTLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            12876..13922
                     /locus_tag="ACHKUI_003728"
     CDS             12876..13922
                     /locus_tag="ACHKUI_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6599848.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEETVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            13935..14291
                     /locus_tag="ACHKUI_003729"
     CDS             13935..14291
                     /locus_tag="ACHKUI_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6599849.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMH"
     gene            14306..15127
                     /locus_tag="ACHKUI_003730"
     CDS             14306..15127
                     /locus_tag="ACHKUI_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6599850.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            15354..15551
                     /locus_tag="ACHKUI_003731"
     CDS             15354..15551
                     /locus_tag="ACHKUI_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6599851.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            15702..16760
                     /locus_tag="ACHKUI_003732"
     CDS             15702..16760
                     /locus_tag="ACHKUI_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6599852.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     HYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            16801..16876
                     /locus_tag="ACHKUI_003733"
     tRNA            16801..16876
                     /locus_tag="ACHKUI_003733"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16834..16836,aa:Ile,seq:cat)
     gene            16886..16962
                     /locus_tag="ACHKUI_003734"
     tRNA            16886..16962
                     /locus_tag="ACHKUI_003734"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16920..16922,aa:Arg,seq:tcg)
     gene            16977..17053
                     /locus_tag="ACHKUI_003735"
     tRNA            16977..17053
                     /locus_tag="ACHKUI_003735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17011..17013,aa:Arg,seq:tct)
     gene            17224..17655
                     /locus_tag="ACHKUI_003736"
     CDS             17224..17655
                     /locus_tag="ACHKUI_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO6599853.1"
                     /translation="MLGIFKKKTRRASAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPTLSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            17652..17816
                     /locus_tag="ACHKUI_003737"
     CDS             17652..17816
                     /locus_tag="ACHKUI_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6599854.1"
                     /translation="MTVKMRLAVVALLLFLVVMVDFSSRIMSVLADGVLVAGVVVVAF
                     PLLKKKASGD"
     gene            complement(17910..18041)
                     /locus_tag="ACHKUI_003738"
                     /pseudo
     CDS             complement(17910..18041)
                     /locus_tag="ACHKUI_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18782..>19860
                     /locus_tag="ACHKUI_003739"
     CDS             18782..>19860
                     /locus_tag="ACHKUI_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6599855.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGAVISTTSNPEYYFVVALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGASCNYNDIIPADHCLHDVQDLSKFSHPKASAAQYGCVG
                     QGLHIAKKLLPFIPANAGILLVPCCRGGSAFLAGDEGTFSESTGASETSARWGVDKPL
                     YKDLLTRTQAALKANPKNILLAVVWMQGEFDLKQGAYATQPGLFDSMVEKYRSDLSEF
                     GGQCLGGSPSSVPWICGDTTYYWKQTYSSQYDAVYGAYKTKSAKKIFFVPFMTDENGR
                     NVGTNEPSEDPDVADIGYYGAGGRT"
BASE COUNT         5210 a         4346 c         5389 g         4915 t
ORIGIN      
        1 cttgtcaaaa gtgacgttca tcggatctga ttcagtctca accgggacaa aggaagcaga
       61 ctcctcatcc cagcggtttt cctgcatata ttcagcatcc caggaatcga gggcagggcg
      121 gggtatgccg ggtttatcct cacagacaat aaatttataa gcgcagtcct gagcagccgg
      181 ataatgttcc aggaattgcc agtgaaattt tgcgcgggcg cgacgttcat caccggcttc
      241 aatggcagtg gctacagcga ctgcaccttc ttcctttatt gcctgttcgt ccggaatggc
      301 ggcgcaaata aagactttac tcattttgtt ttacctcatt acagatttaa gggtgaacaa
      361 atccctgcca ttgctggcat ataagaatga aaccggatat ttattacgga actgttttaa
      421 agacctgccg ggatttcgtt attatcctgg tgaataactt tatcgaccgg gtaacagtta
      481 ccgggaattt tctgttcggt tgctgcagtc acacactcct gcattgtcct gtgaacactg
      541 actgcaatat caactggctc tccggaaaca agaaaaactg tcagaacaag tgcaaatgct
      601 gtattcattg tgcacatcct ttttgtatcg gacgtaaacg ggccagcatt gaaagaatgc
      661 atattttatt taataactcc cgttcgtgtt ttctcttgtt aatggcatct tcagtaaata
      721 cagggttact gatagtgaca ccaatttcaa aacaaccttc agacgtatta acgtttggta
      781 ataacgtttc cattatcgcg tcctcaacaa tgaattttgt gatgcggtgc ctggtgcctc
      841 caggtgacgt taaccagtta acaattaacg ccggatacag agaatccacc cataacactg
      901 tttttggttt taactgttcc gcgtgcgctc agccgcattc accacatcac aaaattcact
      961 ttaaaaaggg cggcagagca gtcacggagt aaaactgata ccgccaaacg tcaccagaaa
     1021 attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca acctgacaac
     1081 ccggtgtcct caacggggga aggaataacc ccgccatact taccgccgcg ccatttcgcg
     1141 gattgccaca accggaagcg cacggtcgac gaaaatttaa cgacaggcta tctatgaacc
     1201 agctacctcg ccgagcgctt tcgcgttatg gtctgacttt tcagggaaat atcctttcag
     1261 taaactgtca gtgccggatg ctcacccgtg tccggcgcac gcactccacc tcacccgtgg
     1321 agaactcctt aatcaccaac cctcaggagg gtgaaatgtc gactgaaaat gatgaaatca
     1381 ttaactccct gatacgccag attaataatt ttgataaagc attgcagcat gctgcggcgc
     1441 gtagtgatat aactctttta gcaatttcat ttcttgcatc tgttatggat aaaaatgaag
     1501 tcgtacgaca gagtcttgtt gattatatcg actcgcttca acccggcact ttcaatcatg
     1561 agaaagagca tgttaagtct gtaattaatt ctcttatttt gaatcaaaag aattaatgct
     1621 ttttgttgca aagtaatttt caaggggttc tattcgaatc cctttcgttt tcattaacaa
     1681 gccaaacccc ttatcaatga tgtccattag atccaggaag tatttttcat gtaaatcctg
     1741 gttatcagag agctgcttct cttcgtacag accgataaag gcacgacgca cgttaccgga
     1801 tatagtatcg atggtttctt tttctacggt actcaggtca agagtcgcca gttgggaacg
     1861 aactatattc gctgccattt cctggaatgg cattggtaaa tctttaaatt ccattattag
     1921 cctcgttggt tagctattaa cgtgggtatg taaccattct ggcaatgctt aatgccgctg
     1981 cttttttcca gcctggtgat atcctgctcc agagcggaca gattttcagc ctgcttagtc
     2041 ctggcttcat tggcccattt cagatcctgc actgcattaa ttttctggcg catccactca
     2101 taaagttcat catcggtata gtctggcgcg atgatgacgg gttctcgttt ctgcatactg
     2161 attcctcgcg gtgctgtttc gcttatcagc cgttagattt tcccgaactg gaaagcgcct
     2221 gtttaaattc actgaagctg agagcttctt cgccttcggc aagaccatcg aagtattctt
     2281 cgtaagcctt ttccatgatt gtgtcgaaat ccatatcact cacctgagtt tctttccagc
     2341 cagcgacggg caccattttc ggttttaaac gttttgcttt tggtatacgt catcgcggtg
     2401 aatgtgccgt cctggttggg aaacacgccg tacaccagag attcgttgtt gccaagctcg
     2461 atagtatcca tgctgacctc atttcccctt aacgccgggt ggcggaacta aaacctacag
     2521 cgccgtgctg ttcttgatag aaatattagt aacgcggata ttttaagtca acagtatggc
     2581 gtatgatatt tttgatttgg taactatgta aatgtttttt caagggaaaa atattagtta
     2641 tacagctgat ttgcagaagt tatggcacaa aaaaaccgac taagacgtcg gtttttttgt
     2701 tgtggatggg gtagtgagcg gtggctactg gttacgtttc tttagtgcca gcatgttctc
     2761 gaaggcttcc tcgtagagct tgtttagtcc acgtagctgg ttaaggagtt tggctttttc
     2821 tgacgcaggt agaatctcga agaggttaag taactctgcc tgttcttcat tgaccagcct
     2881 ccatcctttg cctgaaaagt tatcatcata agtatctgat gatcttacat aattcattaa
     2941 gtctttaagg tcttctcgaa tgtcctctgg ttttaccttt aacagagccg caaattttag
     3001 cgcagcgtcg gtatttaccg gtatcttgcc gttcagatac tggctaacgg tgccttgaga
     3061 ttcgaatccc aacaactcag ccgccagctc ttgagtcagc ttcagctctt tttttcttgc
     3121 attccatgcg gcttttaaat tcttgctcgc ttctggagtt gcaatcactt cgcgtgtttt
     3181 tttcatacat agagtttatt tgttttacca atattatcaa agatagtctg gctattgatc
     3241 tttaaaatta gcggggctaa tatttgctcg aggcataacg tagaaggttg gctatgaact
     3301 taagagacta tttaaaagag aaacatatca cccagctaca gtttgggaag ctaacgggtt
     3361 tatctcaggt gcatgtaagt cgagtgctgg ggggctatga aagattcagc cctgaaaaag
     3421 cattacgtgt tgctgaagta acgaatttcg aggttacacc tcatgaactc cggcctgata
     3481 tttacccgaa tccaaccgac ggcttacctg ttggattcaa ggctaacaca ccaaatgcat
     3541 cggagttgat tcatgaaaat caggcatgag cacatccgca tggcgatgaa tgcctgggcg
     3601 catccggacg gtgaaaaagt tccggcagct gaaataaccc gggcttattt tgagcttggt
     3661 atgacgttcc cggaattata tgacgacagc catccggaag ccctggctcg caatacccag
     3721 aaaattttcc gctgggtgga gaaagacact cctgatgcgg ttaaaaaaat tcaggcgttg
     3781 ttaccagcga tcgaaaaagc aatgccgcct ctgctggtgg cccgaatgcg cagccacagt
     3841 tcagcttatt ttcgggagct ggtggagacg cgggaacgac tggtgagaga cgctgatgat
     3901 tttgtcgcag tggcgatcgc tggtttcaac cagatgaatc gtggtggccc tgcaggaaat
     3961 attgtggctg tgcattgact cgcaatattc ataccggatc acttccggca atttgtgagt
     4021 aaaaagattc ggtatcagaa gaggtgagta tggctaacgc ctggctcaga ttatggcatg
     4081 acatgccaaa tgaccctaag tggcgaacaa ttgccagggt gtcagggcag ccaattgcaa
     4141 cagtgatggc agtgtatatc caccttctgg tgagcgcgtc acgaaatgtc acgacatgtc
     4201 acggcgtgtc actacgtggt cacattgatg tcacgacgga agatttagca agtgcgcttg
     4261 atgtgacgga agaagtaatt gattcaattt tacaggcaat gcaggggcgg gtacttgatg
     4321 gagatttaat caccggatgg gaaaaacgcc aggtactgaa agaggacaat ggcaacgttt
     4381 cacaaaccgc gaaatccccg gcagagcgca agagagcgca gcgcgagagg gaaaaattac
     4441 gaaaacagaa tgaggggtgt cacgacgagt cacgcatatg tcacgacatg tcacgacgag
     4501 tcacgacaga taaagataca gataaagaat taaaccccac acataacgcg cacgtgcgcg
     4561 agagtgctcc gaccagtgag tcgagtggta cgccgttgca ggcagcagaa cctgcatccc
     4621 tggatggact gagcgaaccc atcgggaaat ttccgatggt cgatgactgg catccgtcgc
     4681 cggattttcg acgacgggct gcgttgtggg ggatggcttt gccggagccg gaatttacac
     4741 ctgctgaact tgccgctttc cgggactact gggcagcgga ggggaaagtt ttcacgcagg
     4801 ttcagtggga gcagaaattc gcccgtcacg taaatcacgt cagggcgcag gttaagccag
     4861 tcagcaaggg ggtaaaccat gcagcagcac caggtggcac cgcatcacgg gcagttcagg
     4921 aaattcgggc agcacgtgag cagtgggaac gtgaaaacgg atttatcagc gacggaaacg
     4981 gtctggaagc tgtgggaact catgggggag gtttattcga accgctggac ccagaagaac
     5041 ggggccgcac cttcgaagct ctggattgca cagattggcg cgatgactga gcagcaaatc
     5101 cgacaggtct gccgccagtg catggaccgc tgccgggcgg gtgaaacatg gcctccggac
     5161 ctggctgagt ttgtggcgct gatttcagaa agcggggcca atccatttgg cctgacggtg
     5221 gatgctgtga tggaggagta ccgccgctgg cgtgatgagt cctggcgata tgacggaagc
     5281 gacaaatatc cgtggcctca gcctgtgctg taccacatct gcctcgaaat gcgtaccaga
     5341 gggattgagc gccagatgac gcagggtgag ttaaaacgac ttgcggaacg gcaactgacg
     5401 aaatgggcaa agcatgttgg taacgggatg agtgttccgc cagtgcgacg acaactggaa
     5461 ggggcgaaac acccgcaagg gccaacgcca attgaacggc tgaaacagga atacgaacgc
     5521 cggaaggcag ctggttttat ttgaatctga gaaacgattt tgtcggagga aattttaatg
     5581 gaaaccgtat ttgacgcact gaaagcaatg ggaaaagcca catcggtaga actggccgcg
     5641 cgacttgata tcagtcgtga agaggttctc aacgagctgt gggaactcaa aagaaatggc
     5701 gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc
     5761 gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa
     5821 gttaccgcag acatgatgat tgagtttatc ggtcaggatg gtgctaaaac gtgtgaggaa
     5881 ctggcgggta agttcggcgt cagtactcgc aaggttgcct ccacgctggc ggtggtaacc
     5941 gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc
     6001 gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt
     6061 cctcagccag caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa
     6121 acagaaactg tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat
     6181 gagctgattt ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat
     6241 gttcagaagt gggagcgagt ctgcgccgcg ctgcgggagg tgaacaagca ccgggatatt
     6301 gttcgacaga ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg
     6361 cttatggcaa aagtatttac accagaagag cgggaagaag tgaaggcgcg cattgtggaa
     6421 ttcgtgcgcc tgagcggacg agaaactttt cgacaactgg cagataaaac gggtgtcagt
     6481 aagaccgcta ttcgtcgttt atctggtgcg cttgcggcca gtggtgatgt ctggctctct
     6541 ggttgcgggg tatttccatc agagcaggcg tatcgcgtat ggcgtaagac accggagaag
     6601 gctgctgacc cgacactgat tcgaaagtta cctgacggag aaatacgtcg ttacaacaga
     6661 cggcagaaca taatttgtcg tgagtgccgc cagagcgaag ttatgcagcg tgtgctggcg
     6721 ttctatcggg gaaactttca ggaggtgatg gagtgagggt cagagtttat attgccggtc
     6781 caatgacggg atatgaaaat ttcaaccgtg aggcgtttca caaggcggaa gaggaactga
     6841 aacgggaagg gcataccgtc ttaaacccgg cagtacttcc ggacgggctg acacagccgc
     6901 actacatgga tatttgcatg gcaatgattc gttgtgtgga tgcgatttac atgctgaatg
     6961 gctggcagcg gtcagcgggc gctaaggcag agctggcact ggcggagaaa ctggggcatg
     7021 cagtgattta tcaggaggtg gctcaatgag agaggttaac tatgaggcgc ttcgtgaggc
     7081 agcacaaaac tatcagtcga cgctggcgtg gtatcaggct atcccggaca gcccaaatgc
     7141 tgaacgggat tgtgatgcgg ctcttgctgc gtttaagcgt cacatccgtc atcgggaagc
     7201 ggatattatc gctgatttgc tggatggact ggaagaagca aaatcacaac tcaacgagca
     7261 gcgtgagtat tacgaaggcg ttatctctga tgggagcaag cgtattgctg aactggaagc
     7321 gcgggaagtt caattaccga ctcgctacga ccttcgatat ggacacccga taaatgcaga
     7381 tgagcgacat gtcatgatac ctaaagaaaa tggcagttgg ctttacctga ttgacttaga
     7441 acacgcatta cgcgtcgctg gcattcgcat caaaggagag gagcatggaa ataaaaccag
     7501 aggatgagtt aagcaatatc gttttatttc cggtaaaaga ggatgaccct cgtaatcagg
     7561 ttaattttct ttatgagcca tcggaaagac catattgtca tcacgcctct gttcgggttg
     7621 acgaaaaaga gcgtcaggtc cgctgtaaaa tctgcggtgc agttgtggag ccgtttgact
     7681 ggatgctctc tgtggcgaaa agagaaacca gactggcaga tgatgtaagg ctattgcgcc
     7741 aggaggaaca ggaaaggcgg aaaaatatag aaaagttaat ttagattgag cgtaacgcga
     7801 aagcgcggat atgcagggcg acaaaatcca gaactgaata attaaattta gcactgtaaa
     7861 taaaatcaaa tccttaaccg gagggatttc tgcaccctca gaacatcagg aggccgtccg
     7921 aaagggcggt agtgaaatgc gaaaattcaa aataattatt gaaacgggaa tagccggtgg
     7981 agatttcgag gatgaattcg aagtggatga tgatgcaaca ccagatgaaa tacaggatga
     8041 agctaaagat attttcttta actactgcaa ttactcatac cacgaaataa aagacgaaga
     8101 ggaagaacaa aatggctgat tttggttcaa ctaaatataa cgccagtttt gaagaatggc
     8161 atgaactgtt aatggattat gcagagttac gcggtggaag tgccgctgat gctgaagcat
     8221 ggcgtgatga ttatgaagca gggaaaacac cggtcgaagc atattgtgat gagtggggcg
     8281 atgaatgagc gagattaatt atcaggaagg gcatgaaaag gtagggcagg caaaaccagt
     8341 ggcatggcga tatcgctacg tgaaaaaagg cgttacagac tttcagggga agcagtgggt
     8401 tggtgactgg aaatatgtcc cgacaaaaga ggattgtaac gacagaccaa actatgaaat
     8461 tcaggcgtta ttcacggccc cgccagcttc ggtgacatca gaaggactgg ttaaagctgt
     8521 gcgcttttat gaacaggtac ggcgtgagga tccgccagtt gaaaccggag catggaaaga
     8581 cgctattgac tgggtactca aggaggcctg cctggttgta aacacgggca tcaaaggagg
     8641 ctgaatgggt attgccgcaa gttataccat gcatctctat tgtgattgcc gccagtgtac
     8701 ggatggcaaa tatcagtcgc cagattttgg tgagtatatc ggtacgtcat gggctggctg
     8761 tgcaaaagag gcacgtaaag atgggtggcg aataagtgcc gacaaaacgc gtgcttttgc
     8821 gccagggcat aaaattttga ggagcaacaa aggagagtga tgtgcctaca ttattcagaa
     8881 aagaatatcc gcgaaaaagt agagcgacag aatttttgtt tctcattctg tttatcgtgt
     8941 tgatgatacc gatatccccg ttaattctgg tatggggaat cggaaaaata attgagccag
     9001 ttattgaatt gtataacgac gtggtatggg ctccgttcaa cacactgcac aataaaatta
     9061 atccgtataa ggaaaactga tatggcaact ttgacaaaaa aagaacaggc atggttgaac
     9121 gaattacagg acgttcttga tcgctgtcca tcaccgaaaa aaattggttt ttacaccatt
     9181 ggcgataaaa gcatttacct gtatgacctg cgccgcatgg atgaaatcat ggaggctctt
     9241 gataatcgtt cgtcaatgga ttggtgtgtt gctgtccatg atatgaatgc agggtttgat
     9301 gaaaagattt tgttcccctc atcagttgaa agcactgcgg gttaaggagt aacacatgac
     9361 cactattacc aaagaacgta ttgaattgtt cattaaaaac ccacttgaaa acgggcttac
     9421 ccgtggtgaa caaatggaac tggcacggat tgcgctggca tcgctggaag cagagccggt
     9481 tgtgttctgg tttgaaaaat atcaagaagg ggctacggca tgacgacttt taccagagag
     9541 cagttaatag ctcacgcaga ggagactatt gaagcacaga gactgtgcat accgggcaca
     9601 atcgaccatg acatcatccg cacatataag atggatattg ctgttctgga aatcgcactg
     9661 gtatcgctgg cagcagagcc agccggtaaa ttgcatgaat acaaaccagt gggatatcag
     9721 cgtctggtcg atgagttaac catgctggta aagcagttaa cctggcaact gaggaaagcg
     9781 aagccagact gcaaattacc ggataaggcg atgagttatc tggagcggaa cggactgata
     9841 agcgtggagg atattttacg atgacctggc ctgaagcatt aacaacggta ggaattgcga
     9901 tggcggtggc gctggtggtg tattcgattt gccgctgggg ataaaaacgg tttgcgggaa
     9961 aaggagagtt aagtagaatt gcagcgggtg cttgaggcta tctgtctcag gcatgaacac
    10021 caaaaggcag atagagaaaa gccccagtta acattacgcg tccggcaaga cgcttaacat
    10081 taatctgagg ccatatctat gctctacaca cgtaggttag cctcttacgt gccgaaaggc
    10141 aaggagaagc aggctatgaa gcagcaaaag gcgatgctaa tcgccctgat cgtcatctgt
    10201 ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga
    10261 accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagacccgg
    10321 cgggggagta atctcccgcc acctctgatg tgccaggcat cctcaacgca cccgcactta
    10381 acccgcttcg gcgggttttt gcattagtct ggttgacaaa aatagaaaaa tgcgaaaata
    10441 tgtggtttac gaattctaaa aaaagcgaaa cttgaaatga atgaaaatca gttagctcct
    10501 tgttgggaat ttcaacctta tcttgctgaa aactatgttc gccacttgtt ggcggagatc
    10561 gctaacgtac ttgagcagct gtactatcat aagcacgcat tagacagcaa ctggtctgaa
    10621 ggtgtaaggg cttatgattg ggtcagaaat catcttattc aaaatgaaga tgcaattcct
    10681 ggccttgaga tgatttccaa ggggttggac tatgtagttg ctttaaataa agttccgcta
    10741 caatttacca aagattgcat taataacccc aaaaagaaac atcgtctgcg tcgaaataaa
    10801 gtagagtatg agcagctctc attgtttggt gatgttgagg ctgagcaaga tattacatgg
    10861 cgagtcatag ctgagccttt tttatccgaa gagggcgatg gtgaattaga gtccacactg
    10921 cctcgttggg aggtagctct tgttggattt aatacttatg gtgctcagat tagtatagtt
    10981 tctcatcaat ctacagcatc gatgccgctt atgcctcttg attataacac actccctgac
    11041 gaagcggaga ttaataaggt gcctcttcgt cggcgtacga aggataaaga tttggatgtg
    11101 agcagtgatg gaacatcagg tgaataactt cactgagtat cggggtgata agctcaaact
    11161 agcgagaatg gctgttgggc tttcttgtga agagttagcc gaaaaaattg gcaagacaaa
    11221 gcaattcgtt agcaaactgg agaaggggtg caggccatcg gagcaatgtc ttgagttaat
    11281 atcttcagcg cttatgatta agtccagttt tctttttact gaacgaaaat acgctctgga
    11341 aagtgatgtc tgccattttc ggagtaagaa gtccaggact caaacgctga ctaatagtgt
    11401 attggccagg gctgagattc ttaatattat aatttctgct gttgaaggtg aaatcgaatt
    11461 tcctgacgtt aacataccgg agcacccagg ggctgaatta cttactccga atgatattga
    11521 gcgagtggca gaagattgtc gccgtgcctg gaatttaggt cttggcccta tatcatcaat
    11581 ggttaaattg gcggagagtt taggggtaat cgttgcgcat gttacgggag tcgatgatcg
    11641 tgttgatgct tttactgttc acaataacag gcctgttatc atcaggaaca atgttaaaaa
    11701 aagcatatgt agatttcgct ctgatttagg tcatgaatta gggcatttag taatgcatga
    11761 gggcataacg acaggtgata aacttacgga atcacaagcc gatcactttt cgagcgcctt
    11821 attagttccc aggttatctt tcattaaaga atttccacga atacgaggta agcaattcga
    11881 ctggaatgct ctggttgaat ttaaacttag atggaaaatc agccttaaaa tgtgtattta
    11941 tcgagccagc gcattaggct tattgaccca ggaacaggca agaactggct atatgcatct
    12001 taattccaga gggtatacga gagttgaacc tggtgatgaa cttttgcgcc ctgaagaacc
    12061 cggcatgctg gccgaggcga ttgaaatgct ggatgatgca acctggctaa gaattcttat
    12121 gaaaactggc ttgagccaag atttaattcg tgagttgttc tccatcaaca gacctattac
    12181 aaatccaaga aatattttcc agattgtttg agtatacccg cttcggcggg ttttttgttt
    12241 ttattttcaa cgtgtttgaa gttttggacg gtgccagaat agaatcaaaa atacttaagt
    12301 agcgcgcagg gagaagaggg atggaccccg aacaggggga gtgctattta tctggaagga
    12361 ttctgttgat gaaaatcgaa gaattacgtg aaatttttag tgaagatggc ctctatgctg
    12421 tgcgcgttga gaatgggggt attacctaca cagcgttaat tcctgatgat catgtagtgt
    12481 tatctgttga ggcattcatt gaatacttgg aaagactcgg tttcaaggta gttcgggaat
    12541 aagttataat acgtgagcca gcctgaacaa ctggcaacct gcagcgccat tggagatgac
    12601 aatggcgcat aatttcaaat ttcgcaattc tgattctgcc tttgccagca ggcacgggtg
    12661 gcgttctcac gcattcaaat atgactggta tcagcacgat ccctgtactg aagaacaggc
    12721 cgaatggctg attcataact accgcagacg tggatatgag tttaggaaag ccctcaccct
    12781 cgattatcgt cactggataa tctacgtcag actcccttat tccgaacgcc caccgcgtcc
    12841 gtcccgcaca ttccagcaac gcatctggag gtaacgtgcg ggtattactt cgacctgttc
    12901 tggtaccgga actcgggctg gtgatcgtta agccgggccg tgaatccatg ccggtattcc
    12961 acaatacccg ggtactggtg gagccggaac cgaaaagcat gcgtaatctg ccgtccgggg
    13021 tcgttcctgc cgttcgccag ccgctggcgg aggataaatc attactgcca tttttcagcg
    13081 acgaacgagt gattcgtgct gctggtggcg ctggcgcatt gtctgattgg ttactgcgcc
    13141 atgttaaatc ctgccagtgg ccacacggcg attatcacca cagtgaaacc gtcattcacc
    13201 gttatggtac cggcgcaatg gtgttgtgct ggcactgcga caaccagctg cgcgaccaga
    13261 cctccgaatc actcgggcaa cttgctcacc aaaacctgtc tgcatggatg attgacgtca
    13321 tacgccatgc aatgaatggc tcgcaggaac gggaattatc gctggctgaa ttatcctggt
    13381 gggcggtccg caatcaggtg gcggacgcgc taccggaagc ggtattacgt cgttcgctgg
    13441 ggttgcgtgc ggaaaaaatc cgctcaatgt accgtgaaag cgacatcgta ccgggagagc
    13501 agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg cctcacgccc
    13561 accagcaaaa cccgccacag gaagagacgg tggtcagcat tgccgttgat cctgagtctc
    13621 cggaatcttt catgaaacga cctaaacgtc gccgctgggt taacgagaaa tacacacgct
    13681 gggtgaagac acagccgtgt gcgtgttgtg gtaagccagc cgacgatccc catcacctga
    13741 ttggtcatgg tcagggcgga atggggacaa aatctcacga tattttcacg ctaccgctgt
    13801 gtcgggagca tcacaacgag cttcatgcgg atcctctggc gttcgaagaa aagcatggtt
    13861 ctcaggttga tttaattttt cgttttcttg atcacgcctt tgcaactggc gtgcttgggt
    13921 aaaagaggtg actgatgctc atagatttgg ttttacctta cccgccgacg gtgaacactt
    13981 actggcgacg ccgtggcagc acatatttta tctcggagga gggaaagcgt tatcgccggg
    14041 ctgtggcgct tattgttcgc cagcagcggc tgaaattaag cctgtccgga aggctggcga
    14101 taaaggtgat tgcagagcca ccggataagc gtcgtcgcga cctggacaat atcctgaaag
    14161 caccgctgga tgcgctgacg catgcgggag tgttaatgga cgatgagcag tttgatgaaa
    14221 tcaatatcgt tcgtggtcag ccagtatctg gtggacgtat gggggtgaag atttacccca
    14281 taatgcatta agagcaggtc aaaaaatgaa actggaagat ttaccgaaat actactcccc
    14341 aaaatcccct ggcctgaccg atgcatcggc ctcaacgtca aaagatgcgc tgagtatcac
    14401 tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggt tttctgcgtt
    14461 cctggggaaa atgggcatca gtatgaatga cagggcgcgg gcaacagaat tactggcaga
    14521 ttatgcactc agtcggtgcg atcgtgtggc ggcgttgaga aaacttccgg cagaaataaa
    14581 accggtagtg atgcgcatta tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc
    14641 gagcaaaaaa cagtgcccct gctgtcacgg aaaaaaattt attgaaagcg aggtttttac
    14701 aaacaagatc cagtatccgg atggtaagcc accagtgtgg gcaaagtgca caaaaggcgt
    14761 gtatccgtct tactgggaag aatggaaaaa agtcagggag gtggtaaaag ttgcctgtcc
    14821 ggagtgtggc ggaaagggtg aggtttccac cgcctgtaag gattgccgtg ggcgtggtgt
    14881 cgccattcat cgtgaagagt cggtaaaacg tggtatgcct gttatcagag attgccagcg
    14941 ttgtggtggt cgtggctgtg aaaggctgcc atcaacggag gcatttaatg ccatatgcaa
    15001 agtgacgagt gctatcacgc ttgatacgtg gaaaaaatca gtgaaacgct tttacgatac
    15061 gttggtggtt cggtttgaca ttgaagaggc atgggcggag cggcagttaa agagggtaac
    15121 gcgatagtgt tgttgatttt tcccgaatct gtggtaaatt tgctctaacg atgggcgttt
    15181 tatgcctgac gttagaagat tttttacacc ccgccgcctg gcgggttttt tatgactgaa
    15241 attgcgtcag tacagtaaac gcgctggtgg cggtgaatac ctgtctttca gcttgctggc
    15301 tttttcgaca agagttattg gtgtgtcacg ttaaccggaa aagggaaaaa gacatgctaa
    15361 aacagcagga tatgacagaa accgccagag tggtgtttaa tgaattaagc gttaccgaac
    15421 cggcgacagt cggggagata gcgcagaata cttacctttc acgcgaacgc tgccagttaa
    15481 tactgaccca gctggttatg gcgggtctgg cagactatca gtttggttgt tacagacgcc
    15541 ttcagtcctg aaggcttttt tatttgtggt aaatgggcgg ctggtgggtg ttaggggcac
    15601 tcaccagcca tctgctcatg cgtctggatc acaagcaaac ctcaggccca ctgctttgcg
    15661 caaaagcaga atgagcctat cagagacagg tttaatgatc catgcttaat actgtaaaaa
    15721 tatccagttg tgagttaatc aacgccgact gcctggaatt tatccggtcg ttacccgaaa
    15781 attctgttga cctgatagtc acggacccgc cgtactttaa agtgaagcct gagggctggg
    15841 ataaccagtg gaagggcgac gatgattacc tgaagtggct ggaccagtgt ctggcgcagt
    15901 tctggcgggt gctgaaacct gccggaagtc tttacctgtt ctgtggtcat cgcctggcat
    15961 ctgatatcga aatcatgatg cgtgaacgct tcagtgtgct gaaccatatt atctgggcaa
    16021 agccgtccgg acgctggaac gggtgcaaca aggaaagcct gcgggcgtat ttccccgcca
    16081 cagagcgcat tctgttcgcg gaacattatc aggggccgta tcgtccgaaa gatgccgggt
    16141 atgaggcgaa gggcagggca ctgaaacagc atgtgatggc cccgctgatt gcttactttc
    16201 gtgatgcgcg cgctgccctg gggataacgg caaaacagat agtggatgcc acaggaaaga
    16261 aaaacatggt gtcgcactgg ttcagtgcca gtcagtggca gctgccgaac gaaagtgatt
    16321 atctgaaatt acaggcgctg tttgcccggg tggcagaaga gaagcatcag cggggtgaac
    16381 tggaaaagcc ccaccaccag ctgctggaga cgtatacttc actgaaccgg cagtatgcgg
    16441 aactgcagag tgaatataag catctgcggc ggtattttgg cgtgacggcg caggtgccgt
    16501 acacggatgt gtggacgcat aaaccggtgc actactatcc cgggaaacat ccgtgcgaaa
    16561 aaccggcaga aatgctgcag cagataatca gtgcgagcag tcgtccgggt gacctggttg
    16621 cagatttctt catggggtcg ggttcgacag tcaaagccgc gatggcgctg gggcgtcgtg
    16681 ctacaggcgt tgagctggag actgaacgtt ttgaacagac ggtcagggaa gttcaggatt
    16741 tagccagtca gaacggatga tattgcagga ttagttacgt accgttatta tcctgcgccc
    16801 ggccctttag ctcagtggtg agagcgagcg actcataatc gccaggtcgc tggttcaaat
    16861 ccagcaaggg ccaccatatc acataccgcc attagctcat cgggatagag cgccagcctt
    16921 cgaagctggt tgcgcggggt tcgagtcctc gatggcggtc catttatcgg cattctgcgt
    16981 tgttagctca gccggacaga gcaattgcct tctaagcaat cggtcactgg ttcgaatcca
    17041 gtacaacgcg ccagacttat ttttcccggc tcgcttttgc gggccttttt tgtatccgcg
    17101 ccacgcccgg cgcatatcaa ccacagagcc tttcgggggt gagcttacgg agtggtcagt
    17161 gtgactttct ctgtgggcag atcgctcccg ggcgttggct cacccaccca aaggaacgtc
    17221 acaatgcttg gaatcttcaa aaagaaaacc cgcagagcgt ctgcggaaat taaaaagttt
    17281 gaaaaacgcg atctggcaca ggcagtgatt aacgctgcat acctggtggc ttatgccgat
    17341 ggtgaatgtg aggcttccga gaaagcgaag atcgaacagg tattacgtaa ccagcccacc
    17401 ttgtccgcgt ttacgtcaga aattaatgcg attagcgcaa ccattatcgg tcagctggat
    17461 acgaacttta aaattggtcg tcgtgcggcg ttacgtgaga tcgaggatgt gaaacacgat
    17521 acgcgtgaag cggaagacgt gctggatgtg gcggtggcca ttgccgaagc agacggcgaa
    17581 atagagccgg aagagcgcaa ggtgctggaa gagattgccg gtgttctggg ccttcgtctg
    17641 gagaatcatc tgtgacggta aaaatgcgtc tggctgtggt tgcgctcctg ctgtttctgg
    17701 tggtgatggt ggacttcagc agccggataa tgtcagtgct ggctgacggt gtgctggtgg
    17761 cgggtgtggt ggttgttgct ttcccgttgc tgaaaaagaa agcatcaggc gattagcagg
    17821 gtatcagtta cccgttgaaa tttttaaata cctcacaatt caggcggttg actgttgtct
    17881 ggtttgcggg gagtttgtta aaagaaactg gcatggtgaa tccccctgtg cggaggggca
    17941 atcagcaact ggtgttttgt caccgaccct tatcctttct gtgcgggttc aggtgctgat
    18001 actgaactca ccgggaggca cccggcacca tgcaatggca catagtgcca ctctccagcc
    18061 cctctccgga ggggcttttc tgtgccggat acatcacagt ttctggaacc ttaggtacta
    18121 cagtatcagt cagggtgcta tattttcaga tgtgatgaaa gcctgtcagc aggcagggcg
    18181 tatcggaaat gacccagtag agaaaacgtt gactcagata ccggtgctga gttaccggga
    18241 aaccggcatc acatgaccgc tatccttcca ggcccatccg ctccggtggg cctttttact
    18301 gcagaaaaca ggttccccgt taaatgctat gttgctcaca attcagtaag ttgacagttg
    18361 cctgtcagac tgggcatttg ttaaaaaaat ttcgcatggt gaatccccct gagcggaggg
    18421 gcgactggtg acggtataat ctctgattat caaaacgaga atgacgcggg tttagtggca
    18481 ccgggctgaa ctcaccggga ggcacccggc accatgtgca tgatgataca gatacgcggc
    18541 tttagcccct ctccggaggg gctttcttgt gggcaaaaaa agcccgagtg ggttcgggca
    18601 acagcatgag atatacattt ttataatcga atggatttta accagaattc ataaggctgc
    18661 gcaactgcgc ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta
    18721 acttccggac ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt
    18781 catggcattt aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg
    18841 actgacacaa aaactgaagg aggggtggca gccatttggc agtccggtgg ccatcacgcc
    18901 ttataccctg atgcaggcca ttgcggcgga aggtgatgtc accacacctg tggtggtgaa
    18961 gccgtcggat ggagaaggcg cagttatcag caccaccagc aacccggagt attactttgt
    19021 tgttgccctg gccgggcagt caaacggtat ggcgtatggt gaagggcttc cgctgccgga
    19081 gacatatgac cgtccggacc cgcgtattaa acagctggcg cgtcgcagca ctgtcacgcc
    19141 gggtggtgcg tcctgtaact acaatgacat tattcctgcg gaccactgcc tgcatgatgt
    19201 tcaggatttg agtaagtttt cacacccgaa agccagcgca gctcagtatg gatgcgtggg
    19261 gcagggatta catatcgcga agaaattgtt gccgtttatt ccggcgaatg ccggtattct
    19321 tctggttccg tgctgccgtg gtggttctgc atttttggcg ggcgatgaag gtaccttcag
    19381 cgaatccacc ggcgcaagcg agacctcggc acgctggggt gttgataagc cactgtacaa
    19441 ggacctgctt acccgtactc aggccgcact gaaggccaac cctaaaaata ttctgcttgc
    19501 agtggtctgg atgcagggcg agtttgattt gaaacagggt gcatacgcca ctcagccggg
    19561 gctgtttgat tccatggtgg aaaaatatcg ttctgacctg tcggaattcg gaggtcagtg
    19621 tctcgggggc tctccgtcat cggttccctg gatttgtggc gacacgacct actactggaa
    19681 gcagacttat tcttcgcaat acgatgcggt gtatggtgca tacaagacga aatccgcaaa
    19741 aaaaatcttc tttgtgccgt ttatgacgga tgaaaacggg cgaaatgtgg gtaccaacga
    19801 gccgtcagaa gatccggatg ttgcggatat tgggtattac ggagccggtg gtcgaacgga
//
LOCUS       DAZFRE010000089        19697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFRE010000089 DAZFRE010000000
VERSION     DAZFRE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.089"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            104..646
                     /gene="yfaZ"
                     /locus_tag="ACHKUI_003740"
     CDS             104..646
                     /gene="yfaZ"
                     /locus_tag="ACHKUI_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6599856.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            746..1948
                     /gene="yfaY"
                     /locus_tag="ACHKUI_003741"
     CDS             746..1948
                     /gene="yfaY"
                     /locus_tag="ACHKUI_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6599857.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2168..2950
                     /gene="yfaX"
                     /locus_tag="ACHKUI_003742"
     CDS             2168..2950
                     /gene="yfaX"
                     /locus_tag="ACHKUI_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6599858.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2965..4170
                     /gene="rhmD"
                     /locus_tag="ACHKUI_003743"
     CDS             2965..4170
                     /gene="rhmD"
                     /locus_tag="ACHKUI_003743"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO6599859.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSENGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4227..5516
                     /gene="yfaV"
                     /locus_tag="ACHKUI_003744"
     CDS             4227..5516
                     /gene="yfaV"
                     /locus_tag="ACHKUI_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6599860.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGGPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5534..6337
                     /gene="yfaU"
                     /locus_tag="ACHKUI_003745"
     CDS             5534..6337
                     /gene="yfaU"
                     /locus_tag="ACHKUI_003745"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO6599861.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6378..6611)
                     /locus_tag="ACHKUI_003746"
                     /pseudo
     CDS             complement(6378..6611)
                     /locus_tag="ACHKUI_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6612..7523)
                     /gene="rpnE"
                     /locus_tag="ACHKUI_003747"
     CDS             complement(6612..7523)
                     /gene="rpnE"
                     /locus_tag="ACHKUI_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6599862.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTITERLRQEGHQIGWQEGKIEGLHEQAIKIAL
                     RMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7716..8906)
                     /gene="glpC"
                     /locus_tag="ACHKUI_003748"
     CDS             complement(7716..8906)
                     /gene="glpC"
                     /locus_tag="ACHKUI_003748"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6599863.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8903..10162)
                     /gene="glpB"
                     /locus_tag="ACHKUI_003749"
     CDS             complement(8903..10162)
                     /gene="glpB"
                     /locus_tag="ACHKUI_003749"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6599864.1"
                     /translation="MHFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRIIPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10152..11780)
                     /gene="glpA"
                     /locus_tag="ACHKUI_003750"
     CDS             complement(10152..11780)
                     /gene="glpA"
                     /locus_tag="ACHKUI_003750"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6599865.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGINAEAIEPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12053..13411
                     /gene="glpT"
                     /locus_tag="ACHKUI_003751"
     CDS             12053..13411
                     /gene="glpT"
                     /locus_tag="ACHKUI_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6599866.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13416..14492
                     /gene="glpQ"
                     /locus_tag="ACHKUI_003752"
     CDS             13416..14492
                     /gene="glpQ"
                     /locus_tag="ACHKUI_003752"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6599867.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPANIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14695..15345
                     /gene="inaA"
                     /locus_tag="ACHKUI_003753"
     CDS             14695..15345
                     /gene="inaA"
                     /locus_tag="ACHKUI_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6599868.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKVEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15399..15653)
                     /gene="yfaE"
                     /locus_tag="ACHKUI_003754"
     CDS             complement(15399..15653)
                     /gene="yfaE"
                     /locus_tag="ACHKUI_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6599869.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15653..16783)
                     /gene="nrdB"
                     /locus_tag="ACHKUI_003755"
     CDS             complement(15653..16783)
                     /gene="nrdB"
                     /locus_tag="ACHKUI_003755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6599870.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(16973..19258)
                     /gene="nrdA"
                     /locus_tag="ACHKUI_003756"
     CDS             complement(16973..19258)
                     /gene="nrdA"
                     /locus_tag="ACHKUI_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6599871.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPVIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4533 a         4873 c         5355 g         4936 t
ORIGIN      
        1 taagtgacta gatccctttt ttcgctcatt gaatcctctg cgccaggcag tacaatcccg
       61 cagctttggg aattttgatt aattgcttgt gggaaagagg ataatgaaaa aaattgcgct
      121 agcaggtctg gccggaatgc tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg
      181 ccaggcgggt aaagaataca ccaatattgg tgtcggtttt gggactgaat cgacgggcct
      241 ggcgttaagc ggtaactgga cacataacga cgacgacggt gatgccgcgg gcgtggggct
      301 ggggttgaat ctgcctctcg ggccattaat ggcgaccgtt ggcggaaaag gcgtttacac
      361 caacccgaac gatggtgatg aaggttatgc cgcagcagtt ggcggtggtt tgcagtggca
      421 aattggtgac agcttccgct tgtttggtga gtattactac tctccggatt cgctctccag
      481 cggaattaaa agttatgaag aagcgaatgc tggcgcgcgt tacaccatta tgcgtccagt
      541 cagcattgag gctggctacc gttacctgaa cctggcaggt aaaaatggca atcgtgataa
      601 cgccattgct gatggtccgt atgttggggt aaatgccagc ttctgacgcc ttccggcgcg
      661 gcgttctgtc gcgccattca gtttcttact acgccctgtc tccctttgcg ttatagtgat
      721 tcgataacaa aagcaggaga taaccatgtt aaaagtggaa atgttatcca ccggggatga
      781 agtgttacac gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt ttttctttca
      841 tcaggggttg ccattatctc gccgcaatac ggtgggggat aaccttgatg acttagtcac
      901 cattcttcgc gaacgtagtc agcacgccga tgtgctgatc gttaacggcg ggctgggacc
      961 gaccagtgat gatttaagcg cactcgccgc tgcgacagca aaaggtgaag gcctggtgct
     1021 gcatgaagcc tggctcaaag agatggaacg ctatttccac gaacggggac gagtaatggc
     1081 accgagcaac cgtaaacaag cggagttgcc tgccagtgct gaatttatca ataacccggt
     1141 aggcactgcc tgtggttttg ccatacagct taatcgttgc ctgatgttct ttacccccgg
     1201 cgtaccgtca gaatttaagg tgatggtcga gcacgaaatc ctgccgcgcc tgcgcgagcg
     1261 tttttcttta ccacagccgc cggtttgcct gcgtttgact acctttggtc gttcggaaag
     1321 cgatctggca caaagcctgg acactctaca actgccgccg ggcgtaacaa tgggctatcg
     1381 ctcctcaatg ccgattatcg aactgaaact caccggaccg gcaagcgagc aacaggcgat
     1441 ggaaaaactg tggctggacg ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac
     1501 cgaaggactg cccgcgcaga tcagtcgcga attgcagagc cgccagttca gcctgacgct
     1561 gagcgagcaa ttcaccggtg gtttattggc tttgcaactt tctcgcgcag gtgctccatt
     1621 gctggcgtgt gaagtggttc cttcacagga ggaaaccctg gcgcaaacgg cgcactggat
     1681 tacagaacgg cgggcgaacc attttgctgg gttggcactg gctgtttctg gtctggaaga
     1741 tgagcatctc aactttgcac tagccacgcc agacggcact ttcgctttgc gtgtacattt
     1801 cagtgccaca cgctacagtc tggctattcg ccaggaagtc tgcgcaatga tggcgcttaa
     1861 tatgctacgc cgttggttaa acggccagga catcgccagc gagcatggct ggattgaggt
     1921 tgttgagtcc atgaccttat ctgtctgatt ccgccactct gtgccggttt acagcaccgg
     1981 cacattcccc ctccggcaaa atttgtccaa cctgttgtac tcaataagaa aaaatccgtg
     2041 cgctgattca caaatttgtg cgtaatttct ccagtccgct ggacgagaaa cgggatttga
     2101 tgcgcttcac aattttactc cccctgagca acgagactga ttccagtatc taagactgga
     2161 gtccagtatg ttggaatcaa gcaaagttcc cgccctgaca cgggctattg atattctcaa
     2221 tttgattgcc cggattggcc cctgtagtgc tgcgacgatc attgacacat tggggatccc
     2281 taaaagcaca gcctatttgc tgcttaatga actcaggcgt cagcgttttc ttagccttga
     2341 tcaccaggaa aatttttgtt tgtggaccag gctggtggag ctttccggtc atgcgttaag
     2401 caagatggat ctccgggaac tggctcgacc gcgtttgacg caactgatgg ataccaccgg
     2461 gctgttatgt cacctgggga ttattgataa cggcagcgct tattacattt tgaaagtgga
     2521 gtcatcagcc actatcagcg tgcgttccca tgaaggaaaa agcctttcgc tttatcgctc
     2581 cggaataggt aaatgcctgc ttgcctggca acctgaagct gtacagaaaa gtattatcga
     2641 agagctggtc tgggagcaga tgacacccac caccattacc catccacaac ggctctacga
     2701 ggaactggcg cgtattcgcc gtcagggctg gagctatgac aacggcgaag attatgccga
     2761 tgtccgttgt gtcgctgcgc cggtgtttaa cgccaataac gagcttaccg ctgcgatttc
     2821 ggtggtcggt acccgcttac aaatcaatga agaataccgt gattatctgg cgggcaaggc
     2881 cattgcctgc gccagggata tttcacgttt gttgggatgg aaaagtccct ttgacttaca
     2941 ggcctcataa caatggagaa catcatgacc ctaccaaaaa tcaaacaggt tcgcgcctgg
     3001 tttactggcg gtgcgacagc agaaaaaggc gctggcggcg gtgattatca cgaccagggg
     3061 gcgaatcact ggattgatga tcatattgcc accccgatga gtaaataccg cgattacgag
     3121 cagtcacgcc agtcatttgg cattaacgtt cttggcacgt tgattgttga agttgaagca
     3181 gaaaacggcc agaccggatt cgctgtttcg acagccggtg aaatgggctg ttttattgtc
     3241 gaaaaacatc ttaaccgttt cattgagggt aaatgcgtca gtgatatcaa actgatccac
     3301 gatcaaatgc tcaatgccac cctgtattac tccggctctg gtggcctggt gatgaatacg
     3361 atttcctgtg tcgatctggc tctgtgggat ctgttcggca aagtggtcgg gcttccggtt
     3421 tataaacttt taggtggtgc agttcgtgat gaaattcagt tctacgccac aggtgcgcgt
     3481 ccggatctgg caaaagagat gggctttatc ggtggcaaaa tgccgacgca ctgggggcca
     3541 catgatggtg atgcggggat ccgcaaagat gccgctatgg tcgcggatat gcgtgaaaaa
     3601 tgcggtgagg atttctggtt aatgctcgac tgctggatga gtcaggacgt gaactatgcc
     3661 atcaaactgg cccacgcttg cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg
     3721 ccacagcagt atgaaggtta tcgcgaactg aaacacaacg caccagcggg gatgatggtc
     3781 accagcggtg agcaccacgg cacactgcaa tctttccgta cgctttcaga aaacggtatc
     3841 gacattatgc agccggatgt tggctggtgc ggtggcttaa ccacgctggt ggaaattgcc
     3901 gcaatcgcca aatccagggg gcaactggtg gtgccacacg gttcgtctgt ttactcccac
     3961 catgcggtga tcaccttcac caatacgcca ttcagcgaat tcctgatgac cagcccggat
     4021 tgttcaacga tgcgtccgca atttgatccg attctgctta atgagccagt accggtgaat
     4081 ggtcgtattc ataaatcagt gcttgataaa cccggtttcg gcgtcgaact caatcgtgac
     4141 tgcaatttga aacgccccta cagccactaa tcactccggg cgttgctgtg acaacggtaa
     4201 cgcccacata ttctaaggag ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga
     4261 aaaaccgcgc gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc
     4321 gttcgaatat tggttttgcc aaacagacct accagattga taccgggctg agtaatgaag
     4381 cttatgcgct gggagcaggc attttctttg tggtatacgc gtttctgggg ggtccggcga
     4441 atcttttgat gcgcaaactg ggggcaagaa cctggattgg tacgacaaca ctgctgtggg
     4501 gatttctttc ggcagccatg gcatgggccg atactgaagc gaaatttctg attgttcgca
     4561 ctctgcttgg tgctgcggag gccgggtttt tccctggtat gatttatctc acctcgcaat
     4621 ggtttccgca gcgtaatcgc gccagcatta tggggctgtt ctatatgggc gcaccgctgg
     4681 cgttaacact gggatcaccg ctttctggcg cgctgctgga gatgcatgga tttatggggc
     4741 atcccggctg gttctggatg tttgttattg aaggattgtt ggcagtcggc gctggggtat
     4801 tcacattctt ttggcttgat gacacaccgg agcaggcacg ttttctgagt aaacaagaaa
     4861 aaacgttgct tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact tctcgattga
     4921 gcgatgcgct gcgtaatggc cgagtctggc aactggcgat tatctacctg accattcagg
     4981 tagcggttta cggattaatt ttcttcctgc cgacccaggt tgctgcattg ctggggacaa
     5041 aagtgggctt tacggcgtcg gtggtcaccg ccattccgtg ggttgcggcc ttgtttggaa
     5101 cctggcttat tccgcgctac tccgataaaa ccggcgaacg gcgtaatgtc gcagcgctga
     5161 cattactggc ggcgggcatt ggtattggtc tgtccgggct gctttctcca gtactggcga
     5221 tcgtagcgct gtgtgttgca gccatcgggt ttattgccgt gcaaccggtg ttctggacga
     5281 tgccgacaca gcttctttcc ggtacggcgc tggctgcggg gattggtttt ataaacctgt
     5341 ttggtgcagt gggcgggttt attgccccga tcctgcgcgt gaaagcagaa acgttatttt
     5401 ccagcgatgc ggcgggatta ctgacgctgg cagcggtggc ggtcatcggt tcgctgatta
     5461 ttttcactct gcgtgtaaat cgcactgttg cgcagaccga cgtggcacat cattaaatag
     5521 tttaaggaac acgatgaacg cattattaac caatcccttt aaagaacgtt tacgcaaggg
     5581 cgaagtgcaa attggtctgt ggttaagctc aacgactgcc tatatggcag aaattgccgc
     5641 cacttccggt tatgactggt tgctgattga cggggagcat gcgccaaaca ccattcagga
     5701 tctttatcac cagctacagg cggtagcgcc ctatgccagc caccccgtga tccgtccggt
     5761 ggaaggcagt aagccgctga ttaaacaagt cctggatatt ggcgcgcaaa ctctactgat
     5821 cccgatggtc gatactgccg atcaggcacg tcaggtggtg tctgccacgc gctatcctcc
     5881 ctacggtgag cgtggtgttg gggccagtgt ggcacgggct gcacgctggg gacgcattga
     5941 gaattacatg gcgcaagtca acgattcgct ttgtctgttg gtgcaggtgg aaagtaaaac
     6001 agcactggat aatctggacg aaatcctcga tgtcgaaggg attgatggcg tgtttattgg
     6061 acctgcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca
     6121 gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct
     6181 ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt
     6241 tggcgttgac acgatgctct acagcgatgc cttggatcaa cgactggcga tgtttaaatc
     6301 aggcaaaaat gggccacgcg taaaaggtag ttattgatat caaaggccca tggggatcgg
     6361 ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgagtg
     6421 gccgcgagca cctggtcacg atcaatgccc tgctccagca tgcgcaaagc aattttaatg
     6481 gcttgttcat gcatgccttc ctgcaaacct tctaatttac cttcttgcca gccttcaatc
     6541 ttaccttcct gccagccaat ttgatgtcct tcctgccgta gccgttcagc aatagtcatc
     6601 aatctctcct tttaatgaca gttcggaata tcagcatcag tgagttgggt tgccgccagc
     6661 acaatctcac gttcaaagcc ctgctccagc atgcgcaagg caattttaat cgcttgttca
     6721 tgcaaacctt caattttacc ttcttgccag ccaatttgat gtccttcctg ccgtagccgt
     6781 tcagtaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat ctcctgaata
     6841 aagcgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag tgtttgtagc
     6901 tgactgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac cataccgatt
     6961 aaatcgcggt cgcgaatatg cttttgaatc agttccagca gagctatacg ccgatgttgc
     7021 atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc ggtgtacaac
     7081 tgccgggcaa gttgcggatc gtcaaactca tccagccagt tgagtgagta cgggtagggc
     7141 gaggcttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc atagccttta
     7201 tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc catattcttt
     7261 tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccctc gctggtttcc
     7321 acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa actggtgggt
     7381 tccaggcgta aggtttgcag gttgcaaagc ttgcgcagcg gctctggtag atgtatttcc
     7441 agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaacaccgc atcatgcggg
     7501 gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat gacgcgacat
     7561 tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac gttttcagag
     7621 gaattttcac tgcgttgcaa cgcatacatt gggtacggaa tcgtgtacgc aaaaatgccc
     7681 ggtaacaaca tgcttaccgg gcatcagaaa ggagtttaag ccagcgcctg tgccagcagc
     7741 gtaatcggat gttcgcagcg aagacttgtg gacatctcaa tctgccattt acaggtttcg
     7801 cagtcggtga ccaccagatc tgcgccgctc tcttctatct ggcggaacag tggtgcgccg
     7861 atggcttgtg aagtggggta gttctctttt ttgaaaccgt aagttcccgc aataccgcag
     7921 cactgggaat ccagcaccgt taactcaagc cccgggattt tacgcaacag ctccagggtg
     7981 tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac tttcagcggc
     8041 agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca gcgggtcgcc
     8101 agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg gtattcgtcg
     8161 cgcagggcaa atgtacacgt tgaggaggtg gcaatcaccg gaatgccttt tactcccaca
     8221 gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc ggtaaaaccg
     8281 ttggcgatca gcggtacgcc gcagcatttt tctttgctga gcagttgtac gccggtaccc
     8341 atcgcgttga gcactttaat taaatctttg cctagctgcg gatggttgta gttaacgaag
     8401 cagccgtgaa agaaagcgac ctggtcttta tactgcgctt gctgagccgc cacgctgcga
     8461 taccagcgac ggaacgtacc gaaggagtat ttcggtagcg tgcgacggtg gtctattttt
     8521 aacgctgcat caagcaactg ccgcaccggt ttcagcgagg tagcggtgtt gacgatgggt
     8581 gcgaacggcg tggaaacgct acccatcagg tcggtatgac tcaacacaaa attacgcagc
     8641 gacgggcgtg tggtgtcata tttcgcccgc gcgcgctgga taatatcgcc aatcttcaca
     8701 tcggacgggc aggcgacttc acaacgtttg cagttgatgc aatatttcag cgcctcgtca
     8761 tacagtgcgc catcttttaa acgcagacgc tcgccatccg gcccggcttg ttttggcccc
     8821 ggataacccg gattcacccg gctcaccggg caggcggtgg tgcagacggt gcacttaatg
     8881 cagttttcga agctggtgtc attcattgtt ggcctcctgc gcgttgggca atctgttgag
     8941 cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg atgggatcaa
     9001 atccgcccag caccgaaccg atggcaaaca ggttctcaat ggtttgcccc gcctgcgacg
     9061 ggcgtagcgc ctcatcagtg gttacaccga actgctgcca cggctgcggc gcaaaaaaat
     9121 cgcccttata ccattcaccc cgcgtagcgg tttgtagcac atcaaggccg agaatcggct
     9181 cgcgaatgcc gtcacgttct gccaccagtc cgccgctaaa gaaactgccg ctcgcaagca
     9241 ccgcaaatcg tgggcgcagc gggatatcgg cgtgattgcg cgtccagatt tcattcacta
     9301 cgccattttt acaggtcact tttttcactt catcgcccgg catccacacg ccgccctggc
     9361 gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa ggcggcagcg
     9421 ttggcaaaag catcaatgaa caaggtagtt tttcattcaa ccaatgccac agtttgtcat
     9481 cggccaggcc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg accggaataa
     9541 gtgcatcaag taacaacggc cagttttctt cattatcaag gaagcgggca atattcaccg
     9601 cccggaactc ggtggcgtta ttgcgcagca catccagttc cggcagttca atttcagcgg
     9661 tttcaacttt aagatccaat tcgcgcaatg aagccgccgc aagatgcgcc tgaaaatcca
     9721 tcaggccgct aattcccact acacatattt tcttcgcggg cagcggccag acagggactt
     9781 ctggcgaact tagccaggtt gagcgcagag tgcctagcgg tataatccgc tggtgagcca
     9841 gctctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc gcctggcaag
     9901 cgagatcgag cacgcgttgt ggcccgagaa gagtgtaagg atgggcaggt gcctgctgac
     9961 gcaaagattc cagtccactg tgaatgtctg tcaccggttg accatccggc agatggctca
    10021 gcaaatccag cgatccagat gagaaatgca gcgcgctttg accacgagtg acaatggcac
    10081 agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa tccggcgagg ccgccgccca
    10141 taatgacagt atcaaagtgc atctttctgc tccttctcca gaccacacaa tccctgataa
    10201 acccagcggg taaattcgct ttcgcgcagt gcatctcccc aggcgatggg ttgcacgcct
    10261 ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg
    10321 ttaaaacgtt gcagcagtcc ggcagcgcgg caggcgcaga gctcgccctg gcaggtgccc
    10381 atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa attttctacc
    10441 gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct acggtgcaga
    10501 cggccttcgc tcagccaggc aggcgtgcga tcgccatgac gataaaccgc agaaccgcgc
    10561 agcggggcag gcagggagat gactttacgc aaggtaactt cagcgggttc ctgtgaacca
    10621 ggaagtgcca gatcggcggt cgtacagggg cgcgtgtttc ccagtttgcg gcatacggcg
    10681 tcggtagccc attccgccat cagccgatag gtcatcagtt tgccaccggt gatggtgata
    10741 aacccgtcca gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg gctgacgtta
    10801 cgcccgctcg gatcgtcatc gctggcaacc agcgggcgta cgccagagta ggcccgcaaa
    10861 atgcgcgttt tcgccatcac gggggccagt ttttcccctt cacgcagcag aatatcaact
    10921 tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa agaggtggtg
    10981 ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg tttgcggcag
    11041 cggttgatca catgctgatt aatgcggtga tccatgatca gcagcgatcc tttcgccggg
    11101 aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat cccggcggca
    11161 ttaactacga caggcgcatg aagtgcctga atttcgccgg tgagatggtt acgtacacga
    11221 acaccgcaca ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc ggtaaggata
    11281 acggcaccgt gttctttggc atccagcatg tttgctgctg tcagacgaaa tggatcaacg
    11341 gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg ttcgataatg
    11401 cgcgcttgct gcggctcaat ggcttctgcg ttgatccctg cttcttcgca ggcgcggata
    11461 aaagtggcct ggaaggagag gtcatcttcc ggcagggtga taaacaggcc gttggttggt
    11521 tctacgcagt gacgtgcaat gcgtttcaga atctggtttt cactaataca ttcgcgggcc
    11581 gattccgcat cggttaccgc atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg
    11641 gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc gcgcagggca
    11701 cagtcgcggg caatcccggc tcccgttgcg ccaccgccaa tgataatcac gtcacttgat
    11761 tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca tttagccata
    11821 gtaaaaacat gaattgtttg atttcgcgca tattcgctca caattcgaaa gtgaaacgtg
    11881 atttcatgcg tcattctgaa catttcaacc atcgtattta ataatgtgtg cggtaattca
    11941 catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttctccc
    12001 actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggctat caatgttgag
    12061 tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg atccgactta
    12121 tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgctg cttactattt
    12181 ggttcgtaag aactttgcgc tcgctatgcc ttatctggtt gagcagggat tctcacgcgg
    12241 tgatttaggt tttgcccttt cggggatctc gattgcttat ggattttcga aattcatcat
    12301 gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt tgattctggc
    12361 ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca ttgcggtgat
    12421 gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc cgtgtggtcg
    12481 tactatggtg cactggtggt cgcagaaaga acgtggcggt attgtgtcag tgtggaactg
    12541 tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga tggcctggtt
    12601 caatgactgg cacgcggcgc tctatatgcc cgctttctgt gccattctgg tggcactgtt
    12661 cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ctgccgccga tcgaagagta
    12721 caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga ccgcgaagca
    12781 gatcttcatg cagtacgtgc tgccgaacaa actgctgtgg tatatcgcca tcgccaacgt
    12841 gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc tgaaagaggt
    12901 taagcatttc gcgctggata aatcctcctg ggcctacttt ctttatgaat atgcaggtat
    12961 tccgggcacc ctgctgtgcg gctggatgtc ggataaagtc tttcgtggta accgtggggc
    13021 aaccggcgtc ttctttatga cactggtgac catcgcgact atcgtttact ggatgaaccc
    13081 ggcaggtaat ccaaccgtcg atatgatttg tatgattgtt atcggcttcc tgatctatgg
    13141 ccctgtgatg ctgatcggtc tacatgcgct ggaactggca ccgaaaaaag cggcaggtac
    13201 ggcagcgggt tttaccgggc tgtttggtta cctcggcggt tcggtggcgg cgagcgcgat
    13261 tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa tgattggcgg
    13321 cagcattctg gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatga
    13381 acaattactg caaaaacgca acggaggcta atggcatgaa attgacgctg aaaaacctta
    13441 gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg gcggacagca
    13501 acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag catacgctgc
    13561 cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat ttggtgatga
    13621 ccaaagacga ccatctggtt gttctgcatg accattatct cgatcgtgtt actgatgttg
    13681 ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc
    13741 tggatgaaat taagtcgctg aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag
    13801 tgcagactta tccggggcgt ttcccaatgg gtaaatctga cttccgggtg cacacctttg
    13861 aagaagagat tgaatttgtt caggggttaa accactctac cgggaaaaat atcggtatct
    13921 atccagaaat taaagcgccg tggttccatc atcaggaagg gaaggatatt gcggcaaaaa
    13981 cgctggaagt gctgaagaaa tatggttaca ccggtaagga cgacaaagtt tatttgcaat
    14041 gttttgatgc tgatgagctg aagcgtatta agaatgtgct ggaacccaaa atgggtatgg
    14101 atctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag cagaaacagc
    14161 cggacggaag ctgggttaat tacaattacg actggatgtt taagccgggt gccatgaaac
    14221 aggtggcgga atatgctgat ggtattggtc cggattacca tatgttgatt gaggagacat
    14281 cgcagccggc taatatcaaa ctcactggca tggtgcaaga tgctcagcag aataaactgg
    14341 tagtgcatcc ttataccgtg cggtcagata aactgcctga atacactact gatgtgaatc
    14401 agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gctatttact gatttccctg
    14461 ataaggcagt aaaattcctt aataaagagt aaattaacta ccggatggca cttatgtgct
    14521 atccgatacc actcttcatt aaagtctgtg ggttatcctt aattgcaatt attttgagcg
    14581 agatgacttg ttcccggcat aagatactcg tcaatacaac acgtattatt aagattttcc
    14641 tcagctaaac atctatagtc attgtcgtta ataaaaaacg acaggcgata gggtatggca
    14701 gtttcagcaa agcacgacga atttaatcac tggtgggcaa cagagggcga ctgggttgaa
    14761 gagcctaact atcgccgtaa tggtatgagc ggggtgcaat gcgtcgagcg caacggcaaa
    14821 aagctgtatg taaagcgcat gacgcatcat ctgtttcatt ccgtacgtta tccgttcggc
    14881 cgaccaacga ttgtccgtga ggttgcggta attaaagaac tggaacgggc cggtgtcatc
    14941 gtgccgaaga tcgtttttgg tgaagcggtg aaagttgagg gtgaatggcg cgcgttgctg
    15001 gtgactgaag atatggcggg gttcatcagc attgctgact ggtatgcccg gcatgcagta
    15061 tcgccttatt ctgacgaagt acggcaagcc atgttgaaag cagtggcgct ggcgtttaag
    15121 aaaatgcata gcgttaatcg tcagcatggc tgttgttatg ttcgccatat ctatgtgaaa
    15181 acagaaggta aggcagaagc tggatttctt gacctggaga aaagtcgtcg ccgcttgcgt
    15241 cgtgataaag cgattaatca tgacttccgc cagctggaaa aatatctgga gccgatcccc
    15301 aaagcggact gggagcaggt gaaagcatat tactacgcta tgtaatcgtt gtaggccaga
    15361 taagacgtat aagcgtcgta tctggcgtta aaaccaattc acatctcgat ttcaatatcg
    15421 ccttttgccc ggcaacaaca gggcaaaatt tcccccggct gaataaaagc taacggttcg
    15481 gcaatccagt ccacctggcc tgcgaccagt cgggtgcgac aggagccgca gtaaccttcg
    15541 cggcactggt actcaaccgc cacattgtgg gattccagcg ccgccagaag ggaagggtgt
    15601 tcatcctggc acagcagttg tgtgccagta atgcgcaggg taacgcgggc catcagagct
    15661 ggaagttact caaatcgtcg gtgtccactt ccgcgtcaat ctgcccgacc agataagaac
    15721 tgacttccac ttcctgcgga gcaacctgca cgttatcaga caccagccag gtgttgatcc
    15781 acgggatcgg gttggagcgc gtctggaacg gcagatccaa accgactgcc tgcatacgga
    15841 tattggtgat gtattcaacg tactggcaga gaatgtcttt attcagacca atcatcgaac
    15901 catcacggaa cagataatcc gcccagtctt tctcttgctg tgccgcctgg acaaacaggt
    15961 catagcactc ctgcttacat tcttcggcaa tttccgccat ctccggatca tccgcgccgc
    16021 tgcgcagcag attcagcata tgctgggtgc cggtcaggtg cagggcttcg tcgcgggcaa
    16081 tcaggcgaat aattttggcg ttaccttcca tcagctcacg ttcagcgaat gcgaaagagc
    16141 aggcaaagct gacgtagaaa cggatcgctt ccagcgcgtt aacgctcatc aggcagagat
    16201 acagtttttt cttcagctcg cgcaggctaa cggttacggt tttaccgtta acggtgtggg
    16261 tgccttcgcc cagcagatgc cagtagctgg tcatttcgat cagttcatcg taatagctgg
    16321 agatcccttc cgcacgtttc tggatctgct cgttggtgac gatatcgtca aagacaactg
    16381 acggatcgtt aacgatatta cggatgatat gggtatagga gcgggaatga atcgtttctg
    16441 agaacgccca ggtttctacc caggtttcca gttccgggat cgagatcagc ggcagcagtg
    16501 ccacgttcgg gctgcgaccc tgaatggaat ccagcagcgt ctgatatttc agattgctga
    16561 taaagatgtg tttttcgtgt tcaggcagtg cctggtaatc gatacggtcg cgggagacgt
    16621 caacttcttc cggacgccag aagaaagaga gctgcttttc gatcagcttt tcgaagatgt
    16681 catatttttg ctgatcgtag cgagccacgt tgaccggctg accaaagaac attggttctt
    16741 tgagctgatc atttttcgtc tgtgaaaagg tggtatatgc catgaatgtg tcctgttagg
    16801 agttttatgc cggatgcgct tcgcttatcc ggcctacaaa ccgcatcgta ggctgggtag
    16861 ggcgttcatg ccgcatctgg gcatccagtg ccggatgcgc ttcgcttatc cggcctacaa
    16921 attggacccg taggccgggt agggcgctca cgccgcatcc ggcatctcaa tatcagatct
    16981 tacatgcgcc gctttcgcag ccatcgtcct ggattgacgg caccagatcg tcttgtgcgt
    17041 cttctgcacc gtcacgggtg ttctgataat acagcgtttt aaccccgaat ttgtaggcgg
    17101 tgagcaggtc tttcagcagt tgctgcatcg gcacttttcc tgacgggaag cgtgacggat
    17161 cgtagttggt gttagcagag atcgactgat cgataaattt ctgcatgata cccaccagtt
    17221 gcagatagcc atcgttaccc ggcatttccc acagcagttc ataggcgtcg tgcagatgct
    17281 cgtagtccgg caccacctgg cgcagaatac cgtctttcga cgctttgatg ctgacgtaac
    17341 cacgcggcgg ttcaataccg ttagtggcgt tagagatctg cgaagaggtc tctgacggca
    17401 tcagagcaga aagcgtggag ttacgcaggc cgtgcgtttt gattgactca cgcagtgctt
    17461 cccagtcgta atgcagcggc tcattagcga tggcatccag atctttctta taggtatcga
    17521 tcggcaggat ccctttcgcg taagtggttt cgttaaacca cgggcacgcg ccttgctctt
    17581 tcgccagctc attagacgct ttcagcaggt aatactgaat ggcttcgaag gttttatgcg
    17641 tcaggttgtt ggcgctgccg tcggagtagc gtttaccgtg cttcgccagg tagtaagcga
    17701 agttgatcac accaataccc agcgtacgac gacccatcgc tccacgtttg gcggccggga
    17761 tcgggtaatc ctgataatcc agcagcgcgt cgagtgcgcg tactgccaga attgccagct
    17821 cttccagttc atccaggctg ttaattgcgc ccaggttgaa agcagacagc gtacacagcg
    17881 cgatttcacc gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg gcgatttcca
    17941 ggcacaggtt agactggcgc actggcgcga tgaccggatc aaacgggcta tgggtattgc
    18001 agtggtcaac gttctgaata tagatacgac cggtagacgc acgttcctgc atcatcagcg
    18061 agaacagctc aacggctttc acacgttgct tgcggatgct gtcgtctttc tcatatttgg
    18121 tatacaggcg ttcaaactct tcctgatcgg cgaagaacgc gtcatacagc cccggtacgt
    18181 cggacgggct gaacagagtg atatcttcac ctttcagcag acgggtatac atcagtttgt
    18241 tgatttgaac cccgtagtcc atatgacgca cgcggttgcc ttccacacca cggttgtttt
    18301 tcaacaccag caggctttcc acttccagat gccacatcgg gtagaacagc gttgccgcac
    18361 cgccgcgcac accgccctga gagcaggatt tcaccgcggt ctggaaatgt ttgtagaacg
    18421 gaatacaacc ggtatggaac gcttcgccac cgcgaatcgg gctacccagc gcacgaatac
    18481 gcccggcgtt gatgccgatc ccggcacgct gggaaacgta tttaacaatc gcgctggagg
    18541 tggcgttaat ggaatccagg ctgtcgccgc actcgatcag cacgcatgag ctgaactgac
    18601 gagtcggggt acgcacgccg gacatgattg gcgtcggcag cgaaatttta aatgtggaaa
    18661 ccgcgtcgta aaaacgcttc acgtattgca ggcgcgtttc acgcgggtag ttcgagaaca
    18721 ggcacgcggc aaccagaata taaaggaact gggcgctttc gtagatttcg ccggtcacgc
    18781 ggttctgtac cagatatttg ccttccagct gcttaacggc agcataagag aaggtcatat
    18841 cacggtcgtg atcgataaac gtgtccatct gcttgaactc ttcttccgtg tagtcttcca
    18901 gcagatgatt atcgtatttg cccatctcga ccattttcac cacatggtcg tacagtgcag
    18961 gcggctcaaa ctgaccgtag gcttttttac gcaggtggaa gatcgccaga cgtgcggcga
    19021 gatactgata atccggcgca tcacgggaga tcaggtctgc ggcagccttg ataatggttt
    19081 cgtggatgtc agaggtcttg ataccgtcat aaaactgaat gtgggagcgc agctcgacct
    19141 gggaaatcga aacgttatgc agtccttctg ccgcccaatc cagaacgcga tggattttgt
    19201 cgagattgat gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc tgattcatgt
    19261 atgtcgtacc tgtttttcaa tagcttcatg cagactacct gtagtgaggg aagctgctaa
    19321 gaacactata tatagggggt atgtttggga ttcactgcaa gatagtgtga aactggccct
    19381 cttgcaagtg cataactttg tggataactc aggaaggaaa aagtggcttc cgcgcacctt
    19441 aggtcagaca aggtgtccgg gaaagtcaat gggaagagaa aaatttgtta aaaataaccg
    19501 ttcggtgaat tcttgaacgg taaaatagga taggtaaatt gatatagatg gtttattaat
    19561 cttgttgcta atgattgcca aaattgatct aggcacaagt cgtcacataa tctgatgtat
    19621 cagttgtggt caatatataa gcgagtgtaa gcgattgttg agtcggaaat ggttgtttta
    19681 tattcggtta ttgttaa
//
LOCUS       DAZFRE010000090        19349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFRE010000090 DAZFRE010000000
VERSION     DAZFRE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.090"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..812
                     /locus_tag="ACHKUI_003757"
     CDS             <1..812
                     /locus_tag="ACHKUI_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599872.1"
                     /translation="TLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRM
                     GQIFRKVLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKD
                     GYFLQRGMLLALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNL
                     TLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANG
                     TPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(858..1496)
                     /gene="ompW"
                     /locus_tag="ACHKUI_003758"
     CDS             complement(858..1496)
                     /gene="ompW"
                     /locus_tag="ACHKUI_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6599873.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1735..1824
                     /locus_tag="ACHKUI_003759"
                     /pseudo
     CDS             1735..1824
                     /locus_tag="ACHKUI_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1852..2595
                     /gene="yciC"
                     /locus_tag="ACHKUI_003760"
     CDS             1852..2595
                     /gene="yciC"
                     /locus_tag="ACHKUI_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6599874.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANIRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            2625..3164
                     /gene="yciB"
                     /locus_tag="ACHKUI_003761"
     CDS             2625..3164
                     /gene="yciB"
                     /locus_tag="ACHKUI_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6599875.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3270..3668
                     /gene="yciA"
                     /locus_tag="ACHKUI_003762"
     CDS             3270..3668
                     /gene="yciA"
                     /locus_tag="ACHKUI_003762"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6599876.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3708..4424)
                     /gene="tonB"
                     /locus_tag="ACHKUI_003763"
     CDS             complement(3708..4424)
                     /gene="tonB"
                     /locus_tag="ACHKUI_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6599877.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTTPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            4648..4944
                     /gene="yciI"
                     /locus_tag="ACHKUI_003764"
     CDS             4648..4944
                     /gene="yciI"
                     /locus_tag="ACHKUI_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6599878.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5244..6497
                     /gene="kch"
                     /locus_tag="ACHKUI_003765"
     CDS             5244..6497
                     /gene="kch"
                     /locus_tag="ACHKUI_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6599879.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(6552..6725)
                     /gene="yciY"
                     /locus_tag="ACHKUI_003766"
     CDS             complement(6552..6725)
                     /gene="yciY"
                     /locus_tag="ACHKUI_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6599880.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            6868..8328
                     /gene="cls"
                     /locus_tag="ACHKUI_003767"
     CDS             6868..8328
                     /gene="cls"
                     /locus_tag="ACHKUI_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6599881.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8363..8692
                     /gene="yciU"
                     /locus_tag="ACHKUI_003768"
     CDS             8363..8692
                     /gene="yciU"
                     /locus_tag="ACHKUI_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6599882.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(8745..9749)
                     /gene="oppF"
                     /locus_tag="ACHKUI_003769"
     CDS             complement(8745..9749)
                     /gene="oppF"
                     /locus_tag="ACHKUI_003769"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6599883.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(9746..10759)
                     /gene="oppD"
                     /locus_tag="ACHKUI_003770"
     CDS             complement(9746..10759)
                     /gene="oppD"
                     /locus_tag="ACHKUI_003770"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6599884.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(10771..11679)
                     /gene="oppC"
                     /locus_tag="ACHKUI_003771"
     CDS             complement(10771..11679)
                     /gene="oppC"
                     /locus_tag="ACHKUI_003771"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6599885.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GLLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(11694..12614)
                     /gene="oppB"
                     /locus_tag="ACHKUI_003772"
     CDS             complement(11694..12614)
                     /gene="oppB"
                     /locus_tag="ACHKUI_003772"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6599886.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(12700..14331)
                     /gene="oppA"
                     /locus_tag="ACHKUI_003773"
     CDS             complement(12700..14331)
                     /gene="oppA"
                     /locus_tag="ACHKUI_003773"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6599887.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            14750..14911
                     /locus_tag="ACHKUI_003774"
     CDS             14750..14911
                     /locus_tag="ACHKUI_003774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599888.1"
                     /translation="MSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            complement(15069..15716)
                     /gene="ychE"
                     /locus_tag="ACHKUI_003775"
     CDS             complement(15069..15716)
                     /gene="ychE"
                     /locus_tag="ACHKUI_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6599889.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(15747..15800)
                     /gene="ychE"
                     /locus_tag="ACHKUI_003776"
     CDS             complement(15747..15800)
                     /gene="ychE"
                     /locus_tag="ACHKUI_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6599890.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16193..18868
                     /gene="adhE"
                     /locus_tag="ACHKUI_003777"
                     /gene_synonym="adhC"
     CDS             16193..18868
                     /gene="adhE"
                     /locus_tag="ACHKUI_003777"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6599891.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            18994..>19349
                     /locus_tag="ACHKUI_003778"
     CDS             18994..>19349
                     /locus_tag="ACHKUI_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6599892.1"
                     /translation="MLIGQALDLISRYDSLRNPLTSLGDYLAPELISRCLAESGTVTL
                     HKRRLPLEMMVWCIVGMALERKEPLHQIANRLDIMLPGNRPFVAPSAVIQTRQRLGIE
                     AVRRVFTKTAQLWHNAT"
BASE COUNT         4989 a         4757 c         4541 g         5062 t
ORIGIN      
        1 acacgctgaa acagaaagcg gcccctctca gggtatttga tgaacgtttt ggcaccagac
       61 cgttagatgc cataaccgta aaagatgtgg tatcagtact ggaagagtac aaggccagag
      121 gacataacag aatgggacaa attttcagga aagtactgat cgatgttttc cgggaagctc
      181 agcaaacggg cgatgtcccg ccaggcttta accctgcaga atcggcaaaa aaaccgcagg
      241 tgcggatatc aagacagcga ctgacttttg atgagtggat gatgatttat aacgcagcgg
      301 aaaaggatgg ttacttttta cagcgcggta tgctgctggc actgatgaca ggccagcgcc
      361 tttcagatat ttgcaaaatg caattttcgg atatccggga tggttatctt catgtcgaac
      421 agcaaaaaac aggaacccgg attgccatcc ctctggctct gcgttgcgat aaattaaatc
      481 tcaccctgga tgatgtagtg tcatcctgcc gcgattgcgt tcttagtccg tggctattgc
      541 accaccatca cgcgaaaggg acagctaagc gcggcgggat ggttaagcca gcaacattaa
      601 ccgttgcatt taaaaaagcc cgggattctg tggattacaa ctggcgtgct aatggcaccc
      661 caccctcttt ccatgagcag agatctttat cagagcgatt gttcagagag cagggggttg
      721 ataccaaaat tttgctgggc cattcgaatc aaaaaatgac cgatatttac aacgacgcac
      781 gcggtaagga atggaaaaaa ctggtcattt gatgaccagt tttgcagagg ggttttgcag
      841 aggttttgca gagaaaatta aaaacgatat cctgcggaga acataaacac ccacggatcc
      901 agtcgtaccg agtcttttac ggtagtaaca ccagatttgt acttagccgt ggtatcgata
      961 tccatgtacc agaccgacat attcagcaac cagtcacggt tgatgagata atccagacct
     1021 acttgccccg ccacgcccca tgaatccttc agactaaggt cagataaacc aagaccttca
     1081 ccggtgtcat tgaagtcatt attaaaaaag gtggtgtagt taatacccgc accaatgtaa
     1141 ggacggaatt tactgcctga atcgccaaaa taccattgtg ccatcagtgt tggcggtaaa
     1201 tggtggacag ttgcaatatc cccggtagtg gctgtgccaa ctttatgacg aaacggcgat
     1261 gccgccagta attccacacc aatgttgtcg gtcgccatat aagtaaacgt caggcccagt
     1321 tgagtgttat tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct
     1381 gttggacgta cggttgcaga acctgcacgc ataaaaaatt cgccggcttc atgcgcaaag
     1441 gcactgccag agagaagagt tgctactgcc aaagccgcca ctgttaactt tttcatatcc
     1501 gctccgtcgt tatggttata aaaacgtgac gaatataccc acattggggt aacaaatgat
     1561 cctacacaga tcacattaca tatggtaatt caccctttat tgatctggat taatttagaa
     1621 gttgcattga aaatcctggt taatgtgatt taaatcaatt tttatatatt tcgctacgca
     1681 aatattaact cgctcgccac ttgcagcggt ttgcccacgc ctgaaggcca gcaattacct
     1741 ccgctttaca aagatatgct tttccataca agacctgatg ctgccacccg gggcttaacc
     1801 agggtacaat tgcccgctaa ttaacacctg cataaaactc aaggagagtg catgtctatc
     1861 acggcgcagt ccgtataccg tgacaccgga aattttttcc gtaatcaatt tatgaccatt
     1921 ctgttggtat cgttgctatg tgcgtttatc acagtggtgt tagggcatgt tttctcaccc
     1981 agtgatgcac agcttgcgca gctcaatgac ggcgtacccg ttagcggcag tagtgggttg
     2041 ttcgacctgg ttcagaatat gtcaccggaa cagcaacaaa ttttgctgca ggcttcagcg
     2101 gcgtccactt tttcaggatt aatcggtaac gccattctcg ccgggggcgt aatattaatt
     2161 atccagctgg tgtctgcggg tcagagagtc agtgcactgc gagctattgg tgccagtgcg
     2221 ccgatattgc caaagttatt tattctgatt tttctgacta cccttttagt acagattggc
     2281 atcatgctgg tggtcgttcc gggaattatc atggccattt tactggctct ggcaccggtg
     2341 atgctggtac aggacaaaat gggcgttttt gcctcgatgc gtagcagtat gcggctgaca
     2401 tgggcgaata tacgtctggt ggcacccgca gtactgagct ggttgctggc aaaaacactg
     2461 ttgctgcttt ttgcctcttc ttttgccgca ttaaccccgg aaattggtgc cgtactggcg
     2521 aacaccttga gcaacctgat ttcagccgta ttgctcatct atctgttccg cctgtatatg
     2581 ttgattcgcc aataacctta agcatttgat tacggaatcg taaaatgaag cagtttcttg
     2641 attttttacc gctggttgtc tttttcgcgt tttacaagat ttatgacatc tatgcggcta
     2701 ctgcggcgct catcgtcgcc acggcgattg tgcttatata tagctgggtt cgctttcgta
     2761 aggttgagaa gatggccctg atcacttttg ttctggtggt cgtcttcggt ggcttaacgc
     2821 tgttcttcca caatgatgag tttattaaat ggaaggttac tgtcatttat gccctgtttg
     2881 cgggtgccct gttagtcagc caatgggtga tgaaaaagcc gctaattcag cggatgctgg
     2941 gtaaagaact caccctgccg caatcggtat ggtcgaagct gaatctggcc tgggctgttt
     3001 tctttatcct ttgcggtctg gcaaacatct acatcgcatt ctggctgccg caaaatattt
     3061 gggtcaactt taaagtcttt ggcctgaccg cccttacctt aatcttcaca ttgttaagcg
     3121 gtatctatat ctaccgccac atgccgcagg aagataaatc ctaaccagac tgccagtcct
     3181 tcaggggctg gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc
     3241 tgtgattttc cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg
     3301 atcttgtttt acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg
     3361 gtggttggtt aatgtcacaa atggatattg gcggcgctat tctggcgaaa gaaattgccc
     3421 acggtcgtgt agtgaccgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg
     3481 gggatgtggt gtgctgctat gcacgctgtg tccagaaagg gacgacatca gtcagcatta
     3541 atattgaagt gtgggtgaaa aaagtggcgt ctgaaccaat cgggcaacgc tataaagcaa
     3601 cagaagcatt atttaagtat gtcgcggttg atcccgaagg aaaacctcgc gccttacccg
     3661 ttgagtaata atcaaaagcc tccgatcgga ggcttttgac tttatgctta ctgaatttcg
     3721 gtagtgccgt taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc
     3781 tcataacgcc atctgcgcat cgcatttttc acctcacgct caaacatgtt tgcaggcttg
     3841 gctgagagga tttgtacgtt atccacgcga ccatctggcg tgacatcaaa tttaacttta
     3901 acctgccctt caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt
     3961 aatgcgcgtg gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta
     4021 cttgatgtcg ggcgcgcggg tgtcgtattt tcaaacggtg atgccggacg cgactctacg
     4081 ggtttgacat cgcgttttgg ctgctccacc tttttcaccg gcttcggttt tggcttaggt
     4141 ttcggcttcg gcttttcaat gaccaccgga gcttcttttg gcggttcagg gatcggctca
     4201 ggttccggtt ctggctctac caccggctcc ggtggtggct gaacggcttg tggcggttca
     4261 agatcagcag gcgcaaccat cgtgacagaa atcggctgcg caggcgcagg tagttcaata
     4321 acctgatgta ccgaggtata gagcagaccc gccacaacag caccatgaat gcagaccgaa
     4381 agtaacgtcg gccatgggaa gcggcgaggt aaatcaaggg tcattgaagt cataatcatt
     4441 tcagtagaaa aaccaggccc cgattttaaa tgcaaatagc aatcatattc aataaggcaa
     4501 gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tatgcatggt
     4561 cttaacaaag acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac
     4621 aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct
     4681 gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg
     4741 catgatgaag gtcgtttgtt gaccgccggt ccaatgccag cggtagacag taacgatcca
     4801 ggggctgcgg gatttactgg ctcaaccgtt attgctgaat ttgagtcgct ggaagcagct
     4861 caggcctggg ccgatgctga cccgtacgta gcggcaggag tctatgaaca cgtttcagtg
     4921 aaaccattta agaaagtgtt ctgaatagag attcaatatt taagggcacg gtttttgcaa
     4981 ccgtgttctt gtttggcctt tccttatagg tcggtcagtg cattcaccac cacaagcaga
     5041 taataacttt atctgtattt ctttatatga tgtgaacata ttcaggaaaa tgccttttcc
     5101 tgacgcagag ttgctcactc aaaacctttg attactgcgg ataaagtcat ctactattgt
     5161 ttaacacatt cacgcgccgc atgtatgttc agaaaatgtg cacgttgaga taattaaaca
     5221 aaattgtggt tacagaggta tcagtgagtc actggactac attcaaacaa acagcaacaa
     5281 agttatgggt gaccctacgg cacgacattc tcgcgctggc cgtcttttta aatggattgc
     5341 ttatttttaa aacaatctat ggtatgtcgg tcaatttgct tgatattttc catatcaaag
     5401 cattttcaga gctggatctc tccttgctgg caaacgcccc actatttatg ctcggcgtat
     5461 ttcttgtcct gaactccatt ggcttactgt tccgggcaaa gctcgcatgg gcaatcagta
     5521 tcattttgtt gttgatagcg ctaatttaca ctctgcattt ttatccctgg ctgaaattta
     5581 gtattggatt ttgcattttt acgctggtgt ttttgctgat actgcgcaaa gacttctccc
     5641 acagtagcgc agcagccggg acaattttcg catttattag tttcacgacg ttactgtttt
     5701 actccactta cggtgcgctt tatttaagcg aaggttttaa tccgcgaata gaaagtttga
     5761 tgaccgcgtt ctatttttcg atagaaacca tgtcaaccgt cggctatggc gatattgtcc
     5821 ctgtttctga atcagcacga ttgttcacta tttcggtcat tatttccggc attaccgttt
     5881 ttgccacatc tatgacctca atttttggtc cgcttatccg cgggggattc aacaaacttg
     5941 taaaaggaaa caatcataca atgcatcgta aagatcattt tattgtttgc ggacattcga
     6001 ttctcgccat caatacgatt ctgcaactga atcaacgcgg acaaaacgta acggttatca
     6061 gcaacttgcc tgaagatgat atcaagcaac ttgagcaacg cttaggcgat aacgccgatg
     6121 ttatccccgg tgacagtaat gacagttcag tgttaaagaa agcgggaatc gatcgttgcc
     6181 gggccattct ggcgctgagt gataacgatg cagataacgc gtttgttgta ctctcggcaa
     6241 aagatatgag cagtgatgtc aaaacagttc tcgccgtcag tgatagcaaa aacctgaata
     6301 agattaagat ggtacatccg gatatcattc tctcaccaca actgtttggc agcgaaattc
     6361 tggcgcgagt tttaaatggt gaagagatta ataatgatat gctcgtttca atgttgttga
     6421 actccggtca tggcattttc agcgataacg atgaacaaga aacgaaagct gacagtaaag
     6481 aatcggcgca aaaatagagt ttaaagacgg aagagtaaac ttattgggca ccgcaatggt
     6541 gcccagtaca tttagatatt gtagatcgca aacaataacg agttacgctg tttgtttaga
     6601 atgcacttcc tgatgctgtg gatacgcata tttcggcgcg actgcccctc aagccaacgc
     6661 gatttacgtc ggctagcctg acgctgcatg cgccagcgcc ccacttccgt tctactccgc
     6721 ttcatgttaa ctactctatg caacaacaga atggtcatta tacccccgcc cctgacgctg
     6781 accagtgctt ttcccgcgtt tttatttgcc agatgaatcc atatgcgtaa actcataaca
     6841 atgcgctttc aaaaggattt ctaatctatg acaaccgttt atacgttggt gagttggttg
     6901 gccattctgg gatactggtt gctcattgca ggcgtaactt tacgcattct aatgaaacga
     6961 cgcgcagttc cctccgcgat ggcctggctg ttgattattt acattctgcc gttagtcgga
     7021 attattgcct atcttgccgt tggcgagctc catttaggca aacgccgcgc tgaacgcgcc
     7081 agagcgatgt ggccttccac cgcaaaatgg cttaacgacc ttaaagcctg taagcatatc
     7141 ttcgccgaag aaaatagcag tgtcgctgcg ccattattca agctttgcga gcgtcgtcag
     7201 gggatcgctg gggtcaaagg gaatcagcta caactgatga ccgagtcaga tgatgtgatg
     7261 caggcgttaa tccgcgacat ccagctcgcg cgccataata ttgagatggt gttttatatc
     7321 tggcagcccg gcggcatggc ggaccaggtg gctgaatcat taatggccgc tgcacggcgt
     7381 ggtattcatt gccgattgat gctcgactcc gccgggagtg tggctttttt ccgcagcccg
     7441 tggcccgagc taatgcgtaa tgctggtatt gaagttgtcg aagccttaaa ggtcaatctg
     7501 atgcgtgtgt ttttacgccg tatggacctg cgccaacatc gcaagatgat catgattgat
     7561 aattacatcg cgtacaccgg cagcatgaat atggtcgacc ctcgctactt caaacaagat
     7621 gcgggcgtag ggcaatggat tgatctgatg gcgcgtatgg aaggccccat cgccaccgcg
     7681 atggggatta tttattcctg cgactgggag attgaaaccg ggaaacgtat tctgccgccg
     7741 ccaccagatg tcaatattat gccgtttgag caggccagcg gtcacactat tcacacaatt
     7801 gcttctggcc ccggctttcc ggaagatctc attcaccagg cattattgac tgctgcttat
     7861 tcggctcgtg aatatttgat catgacaacg ccctattttg tgccaagcga tgatttactt
     7921 catgcgattt gcacggcggc gcagcgcggg gtggatgtca gtattatcct tccgcgaaaa
     7981 aatgactcga tgctggtcgg ctgggccagt cgcgcattct ttacagaact gctggctgct
     8041 ggggttaaaa tttatcagtt tgaaggcggg ttactgcata ccaagagcgt gctggtcgat
     8101 ggtgaactaa gtctggttgg caccgttaac cttgatatgc gtagtctgtg gctaaatttc
     8161 gagattaccc tggcaatcga cgataaaggt tttggtgcag acctcgccgc cgttcaggac
     8221 gattatattt cgcgttctcg tctgctcgat gcccgtttat ggctaaaacg tccattatgg
     8281 caacgtgtcg ccgagcgact gttttacttc ttcagcccgt tgctgtaaaa cgtcgccaac
     8341 agaggttaaa caggtagtga ctatggatat ggatctaaac aatcgcctga ctgaagatga
     8401 aacgcttgag caggcctacg atatttttct cgaactggct gccgacaatc tcgatccagc
     8461 tgatgttctg ctgttcaatc ttcagtttga agagcgcggc ggcgcggagt tattcgatcc
     8521 ggcggaagac tggcaggaac atgttgattt cgatttgaac ccggactttt ttgccgaagt
     8581 ggtgattggt ctggcggaca gtgaagatgg tgaaatcaac gatgttttcg cgcgcattct
     8641 gttatgccgc gaaaaagatc acaaactttg ccatatcatc tggcgggaat aaataatcgc
     8701 ttttgacgtg aataagggct gacaactgtc agcccttatt gcttttaaag cggatcgact
     8761 ttcaggcaag aaacggcgtg tctgaaactc ccttccagca caggacgtgt tttggcgcac
     8821 tccggaccgg caatcggaca acgggtacgg aaaacacaac cggaaggcgg gttgatcggc
     8881 gacggtaatt ccccttccag taactggatg gttttgttct tctccagatc cggatcaggt
     8941 atggggactg ccgacatcaa tgccctggtg taaggatgta gcggattgtg gtagacctca
     9001 tcataggtcc ccagttctac cgcatggccg agatacatca ccagcacacg atcggaaatg
     9061 tgttttacca cggccaagtc gtgagcgata aaaattaatg acaatcccat ctcacgttgc
     9121 aactgctgga gcaggttgac cacctgcgcc tgaattgaca cgtccagcgc cgacaccggc
     9181 tcatcacaga taatcagctt cggttcaaga ataagagcac gagcaatccc gatacgctgg
     9241 cactgcccac cggagaactc atgcggatag cggttaatca ggttaggcaa caaaccgact
     9301 ttcagcatca tcgccttcac gcgctcgcga acttcctggc gtgacatttt cggatgataa
     9361 gtacgcagtg gttcagcgat gatctcgccg atggtcatac gcgggttcag cgatgccaac
     9421 ggatcctgga aaatcatctg gatatcactg cgtacggcac gccattcatc gggcttcatg
     9481 cccagcaact ctttacctaa ccaggcaaca tgaccgtcgg tcgctttaac caggccaatg
     9541 atggcgcgag caaaggtgga cttaccgcat cccgattccc ctaccacacc taatgtttcc
     9601 ccttcataca ggcgaagagt tacaccatcg acggctttga gcgttttcgg cggttgccag
     9661 aaccactgtt tgccatcttt gatttcaaag tgcactttca gatcggcgat ttcgaggagg
     9721 acttttcttc cttcggttac agcattcata acagttcctc caccggttta aagcaagcac
     9781 gcagacggcc aggcgtaaac tcttccagcg gcggtgcgct actacaaatg tccatcgcat
     9841 gcggacaacg tggctggaac gggcaacctt tcggtaatcg cagcaggttt ggcggattac
     9901 ccggaatagt caacattgtt tcaccttccg catcgagacg cggcaccgcg ttgagcaaac
     9961 cgatagaata aggatgtacg ggttgataaa agacatcgcg cgcgttgcca tattccatcg
    10021 tgcgcccggc gtacattacc agcactttgt cgcagatccc cgctaccacg ccaagatcgt
    10081 gggtaatcat aatgatggcg gtattaaatt cccgcttcag ttcattcaat agcgtcatga
    10141 tctgcgcctg tacggtgacg tccagcgcag tagtgggttc atccgcaatc agcagcttag
    10201 gtcgacatag caatgccatc gcaatcatga ctcgctgacg catgccgcca gaaaattcgt
    10261 gcgggtacat tttcatgcgt ttacgcgctt ccggcatttt taccgcatcg agcatccgca
    10321 ccgattcttc aaacgcctca gctttgctca tgttcttatg cagcatcagc acttccatca
    10381 actgttcacc gacgcgcata tagggattca acgaagtcat tgggtcctga aaaatcattg
    10441 agatttgttc agctcgcagt ttattgagtt cacgctctgg caaattgagg atttcacgcc
    10501 cattgaaggt tgccgatccg ccaatacgtc cgttggcagc caacagcccc atcaacgcaa
    10561 atgcggtttg cgatttgccc gaaccagact cacctacgat gcccagcgtt tctccggcac
    10621 gtagcgaaaa attcaaatca ttgaccgccg tgacgtcgcc atccggggta ctaaaggtga
    10681 cacgcaaatc tttcacgttc agcagtgcgt cagcctgttg ttgtgcgagc ggcacagttg
    10741 cagtttcaat tacgctcatg gctgcactcc ttaacgatct ttcgggtcga gggcatcacg
    10801 caagccatcg ccgataaagt tgaaacaaaa cagcgtcacc acgaggagtc ccgctgggaa
    10861 caacagtaac catggagaaa cttccatcga gttcgcgcca tcactcagca atgcacccca
    10921 gctgcttaac ggctcttgcg tacctaatcc caggaagcta aggaaagatt caaagaggat
    10981 catgctgggc accagaagcg acgcatagac caccaccaca ccgagtacgt tcggcacaat
    11041 gtggcgaata acaatgcccg gcgtcgatac accgccaact tgtgccgcct caataaactc
    11101 tttgcgcttc agactcaggg tttgcccacg cacaatacga gccatatcca gccaggaaac
    11161 catgccaatc gccacgaaaa tcagcaggat gttttgaccg aaaaaggtca ccagcaaaat
    11221 gacgaagaac atgaatggga aggagttgag gatttccagc agacgcatca ttaccgaatc
    11281 cactttaccg cccagataac cggaaagcga accataaagt gtccccacga ccactgccac
    11341 cagtgccgca gcaacaccga ccatgagtga gatacgcccg ccaatcgcaa cgcgcacaag
    11401 caggtcgcga ccggatgagt cagtaccaaa gtagtgaccg gactccatat ccggggcgct
    11461 ggacatcatc gcccagtcag tatcgtcata ggcaaactgc gaaagcatcg gtgccaggat
    11521 tacaaataac gcgatcagca ccagcactat cagactggca accgccgcac ggttatgcat
    11581 aaaacgtcga cgtgcgtcct gccacaagct gcgcccttcg acctccagct tttcactgaa
    11641 attttccagc gtctcgctgt ttttcttact taacatcatc gcgagctcca gtatcagtaa
    11701 cggattttcg ggtcgataac cgcatatagc acatcgacaa tggcattaaa caaaatggtt
    11761 aacgcgccaa ccagaatggt cagacttaac actaaggaat agtcacggtt caatgcgcca
    11821 ttaacgaaca attgcccaat ccccggtaaa ccataaatgg tttcgatgac catcgaaccg
    11881 gtaataatgc cgacaaatgc gggccccata taggagagca cgggtaacag agcaggtttt
    11941 aatgcgtggc gtaaaatgat ccgccgcata ggtaaccctt tcgcccgggc agtacgaata
    12001 aagttggagt gtaaaacctc aatcatagaa ccacgggtaa tacgcgcaat actggcgata
    12061 taggcgagtg ataacgccac cattggcaat atcatgaatt taagcgctcc accattccag
    12121 ccaccgcccg gcagccaatg caaaatgatc gcaaatatca tgactaataa tggcgcaacc
    12181 acaaaactgg ggataacaac cccggtcatt gccagcccca tcacggtata gtcccatttg
    12241 gtgttttgct ttaatgcggc aataacgcca gcactaacac ccagtattac cgcaagaaaa
    12301 aatgctgcgg ctcccagttt ggcagaaacg ggaaaactgg atgcaaccag gtcattgacc
    12361 gaataatctt tatatttaaa cgatggaccg aaatcgccat gcgccagttg tttcaggtag
    12421 ctgaaatact gagtcatgat cggatcatta agatgatatt tcgcttcgat attggccatc
    12481 acttccggtg gtaaagtacg ttcgccggta aaaggactgc ccggagcgag gcgcatcata
    12541 aagaacgaaa tagtaataag aataaatagc gtcggaatcg cttccagaca gcgacgtaga
    12601 ataaatttta acattgccct accttctggc ctgtgccttc tgtaatgcga taaaaatcag
    12661 acaccgtgga gcaggacact cctgccccac gtattgccat tagtgcttca caatgtacat
    12721 attccgggtg taggtattat ccagcggatc tttgccggta tagccaccaa cccacggttt
    12781 caccaaacgc gcattcacgt agtaataaac aggaacaatg gccgaatcct tatccagctg
    12841 ttgttctgct ttagtgtaca gagctgtacg ctgcgcctcg tcagtcactt tcagcgtttc
    12901 cgccataatg ctgtcaaagg ccgggctctt ataatgcgcg gtattcattg agctattcga
    12961 cagcatggtg ttcaggaaag aagttggttc gttgtagtct gcacaccagc ctgcacgggc
    13021 cacatcaaaa gtgccctggt gacgggtgtc gaggaacgtt ttccactcct ggttaaccag
    13081 tttgacgttt acaccaatgt ttttcttcca caatgaagag gcagcaatcg ccagcttttt
    13141 atgcagatcg gaggtgttat acaacaggtt gatggtcaac ggtttgtctg cggtataacc
    13201 cgcttcagcc agcagttttt tcgcttcttc gttacgtttt tcctggctcc agccaaacca
    13261 ttccggctga gtcaattttg cgccatcagt atatggtgga gtgtaaccat aggcgggcat
    13321 gtcgccctgc gctttcactt tattaacaat gatatcgcgg tccataccca atttcagtgc
    13381 ggtacgcaca cgcacgtcat taaatggcgg tttctggttg ttaatttcgt aatagtaagt
    13441 gcacaggtat ggatcaacgt gaacttcgtc cgggatctct tttttcagct tctggaacaa
    13501 ttcgatcggc atgttgttat aagtcatgtc gatttcacca ctacggtagc ggttgacatc
    13561 ggtaacttca gaagcaatag gcaaataggt tacctggtta ataacggttt tcgcgttgtt
    13621 ccagtaggtc gggctgcgtt caagaacgat tcgttcgttt acgacccaat cttttaaggt
    13681 ataggcaccg ttggtgacga tattaccagg ctgggtccat ttttcgccga atttctcgat
    13741 agcggctttt ggcaccggtg aagttgatgg gtgaacaagt aatttataga agtacggaac
    13801 aggttcactt aaggtgactt ctaatgtgtg atcatcaata gctttcacgc ccagatcggt
    13861 aatcggtttt ttcccttcaa gaatttcatc aataccggcg atatgcccat attgcagata
    13921 actggcatac ggagaagcag tgtttggatc aacagaacgt tgccagctat acacaaagtc
    13981 ttgtgctgta actggcgtgc catcagacca tttcgcatct ttacgcaaat ggaaggtcca
    14041 gactttcgcg tctttattat cccaggattc agcgacgcca ggtgccggat gaccgtcaag
    14101 atcgctgacc agtaagcctt caaacaggtc tcggctgata ttagactccg gaacaccttc
    14161 aattttgtgc ggatctaatg actgaacttc tgaaccattg ttacgtacca gcgtttgttt
    14221 ttccgccagt gtgacacctg cgggtacatc agctgccagc gcgacattcc ctgccataag
    14281 cgcagccaaa acgccagctg ctactaaact tctcttggtg atgttggtca ttgttttttg
    14341 gactccctca ttataattac tggtattaac cagcatgtgt aatcccggat tgggaccctg
    14401 cacaggctcg gagactttct gctgtcaggt tactttatcg ttacgtatta ctatcaccga
    14461 ctttatttat tctggtgatt cagacgatca ccttatgtcg attcgtactg tgtctcccct
    14521 gtcgagacac attattcttt cgcgacgatc tttaattgtt aattattctc attaccaata
    14581 aagaatcgcc tggcggttgc ccggaaagta ccaaatgcgg ttcttactcg ccaatacatt
    14641 ttgcaaaatt gttaagcaat tctcttttat tgaccgtacc agaatctata gcttgcatcc
    14701 atgccttaac aaacatcttt caattcaatc aaatagaggt tggttaagca tgtctctctt
    14761 ttctgattac agttcaagca gtgaaatgca caataattta acaattgatt attatttagc
    14821 actttcctct accaaaggca gtggaattac taatatcatt tcaattattc ttcagcaagc
    14881 ccaggattat gatgtggcga aaataacatg atggtgagga gaagcagtta aggaaatcct
    14941 tataactaac tgtaatttga gaagaatatt tttacctatt ccttttgctt ttgataactg
    15001 cgcaggaaca aagtaaaaat aatgacgaag taaacggagc atggcagctc cgtttcattt
    15061 gaaaggaatt aattaagcag gccggggaaa atccccttaa taccagtaac gataaattca
    15121 atccccaatg ccatcagcaa tagccccata atacgcgtaa tcacgttgat gccggtctgg
    15181 cgtaaaactc gtaccagcca cggtgccatg cggaacaatc cccagcaaca taaagcgaac
    15241 aatgcaatag ccacaaagaa accaaacaga taactaatgc tgtgataacg cgtaccccag
    15301 acgatggtag aactgatcgc ccctggcccc gccatcaacg gcaacgccaa tggcaccaca
    15361 ccaatgcttt cacgtaccgc ggtttctgat ttttcttgct tgttctgttt atcctcacca
    15421 agcttgccgc tgatcatcga catcgctatt gtcaccacca ggatgccacc ggcgatacgg
    15481 aatgaatcaa ttgatatacc aaaaagttgt agaatcgtgt cgccgagaaa aagcgagatc
    15541 cacaagataa tggccacaga caggttggct gtaaggttag ttttgtttcg cgctgccgct
    15601 gtctgataac tggtcatgct gataaagacg ggaataatcc ctaccgggtt gaccagcgca
    15661 aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa aaagggtctg aatcacggtt
    15721 agctccgaag caaaagccgg ataatgttag ccataaataa ggttgaaaag acgcgctgac
    15781 aatacgcctt ttgacagcat ttttcacctc ctaactactt aaaattgcta tcattcgtta
    15841 ttgttatcta gttgtgcaaa acatgctaat gtagccaaca aatcatacta caatttatta
    15901 actgttagct ataatggcga aaagccatgc tgaaaggtgt cagctttgca aaattttgat
    15961 ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc tggaagtgac
    16021 gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc
    16081 gcgagtaagc ttttgatttc catcggttaa gcaaatcatc accgcactga ctatactctc
    16141 gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca ttatggctgt
    16201 tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc agcgtgaata
    16261 tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc tggctgctgc
    16321 agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga
    16381 agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgctt ataaagatga
    16441 aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta tcgctgaacc
    16501 catcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg ctatcttcaa
    16561 atcgctgatc agcctgaaga cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa
    16621 agatgccacc aacaaagcgg ctgatatcgt tctacaggct gctatcgctg ccggtgctcc
    16681 gaaagatctg atcggctgga tcgatcaacc ttctgttgag ctgtctaacg cactgatgca
    16741 ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta aagccgcata
    16801 cagctccggt aaaccagcta tcggcgtagg cgcgggcaac actccggttg ttatcgatga
    16861 aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct tcgacaacgg
    16921 tgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg acgcagtacg
    16981 tgaacgtttc gcaacccacg gcggctatct gttgcagggt aaagagctga aagctgttca
    17041 ggacgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc cagcctataa
    17101 aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga tcggtgaagt
    17161 gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat
    17221 gtaccgtgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg ttgctatggg
    17281 cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc
    17341 ttacttcggt cagaaaatga aaacggctcg tatcctgatt aacaccccgg cttctcaggg
    17401 tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg gttgtggttc
    17461 ctggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac
    17521 cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta tctacttccg
    17581 ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca aacgtgcgct
    17641 catcgtgact gaccgcttcc tgttcaacaa tggctatgct gatcagatca cttccgtatt
    17701 gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagctgacc cgaccctgag
    17761 catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct
    17821 gggtggtggt tccccgatgg acgctgcgaa gatcatgtgg gttatgtacg aacatccgga
    17881 aactcacttc gaagaactgg cgctgcgctt tatggatatc cgtaaacgta tctacaagtt
    17941 cccgaaaatg ggtgtgaaag cgaaaatgat cgctgtcacc accacttctg gtacaggttc
    18001 tgaagtcact ccgtttgcgg ttgtaactga cgacactact ggtcagaaat atccgctggc
    18061 agactatgca ctgaccccgg atatggcgat tgtcgacgcc aaccttgtta tggacatgcc
    18121 gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg aagcttatgt
    18181 ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga aactgctgaa
    18241 agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt
    18301 tcacagtgca gcaactatcg cgggtatcgc gtttgcgaac gccttcctgg gtgtatgtca
    18361 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
    18421 gctgatttgt aacgttattc gctacaacgc gaatgacaac ccgaccaagc agactgcatt
    18481 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgctg accacctggg
    18541 tctgagcgca ccgggcgacc gtactgcagc taagatcgag aaactgctgg catggctgga
    18601 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
    18661 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
    18721 cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg atacctacta
    18781 cggtcgtgat tatgtagaag gcgaaactgc agcgaagaaa gaagctgctc cggctaaagc
    18841 tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca taaacggccc
    18901 cttctggggt aatgccagtc agttaaggat cagttgaccg ctccagtggc tgtgtaagaa
    18961 tccggaaacg ctcactcgtt tccggatttt tttatgctca ttggacaggc ccttgatttg
    19021 atatcccgtt acgattctct gcgtaaccca ctgacttctc tgggggatta cctcgccccc
    19081 gaactcatct ctcgttgcct tgccgaatca ggtactgtaa cgctacacaa gcgccgtctt
    19141 cccctcgaaa tgatggtctg gtgtattgtt ggcatggcgc ttgagcgtaa agaacctctt
    19201 caccagattg cgaatcgcct ggacatcatg ctgccgggca atcgcccctt cgttgccccc
    19261 agtgccgtta ttcagacccg ccagcgtctg ggaattgagg ctgtccgccg cgtgttcacg
    19321 aaaacagcgc agctctggca taacgccac
//
LOCUS       DAZFRE010000091        19239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFRE010000091 DAZFRE010000000
VERSION     DAZFRE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.091"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            3..1514
                     /locus_tag="ACHKUI_003779"
     CDS             3..1514
                     /locus_tag="ACHKUI_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6599893.1"
                     /translation="MSKKFTMTLLSSSLAGLLVMSGGVSAQEEKYTVPYATGNNKWGN
                     TYEVVKTGDNGNFKYEVKEKNNGKRTLFTFDSNGDVIINGSGITYTIHDGALNDFAQT
                     AEKKKNGPSQSHRMTDSVVRDVYNKVYSLQRTKITGFSVEDGENGKVSLGSDAKASGE
                     FSVAVGTGARADKKFATAVGSWAAADGKQSTALGVGAYAYADASTAAGTAAYVDGSAI
                     YGTAIGNYAKVDENATEGVALGAKAKVDKNATEGTALGAKATVTNKNSVALGANSRTT
                     RDNEVYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRL
                     VVEAKKSREYTDSRTTVGVNPDGKLTRAEGATKTIAVNDGLVALSGRTDRIDYAVGSV
                     DRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSV
                     GKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGIAASDGDVSYNMGVNFEF"
     gene            1603..2073
                     /locus_tag="ACHKUI_003780"
     CDS             1603..2073
                     /locus_tag="ACHKUI_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599894.1"
                     /translation="MKVILATRNRYLEYGLQALLKGHSVILAREFFLPENRRYIPDFD
                     ESWLIISDGLLGRLMRCMFQGRHFLQLDAELLRDGEQISDAIHNGVWTYNSAARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGINTKTVNTFLYTGMAKNGLYGVSVRRLVGA"
     gene            2106..2255
                     /locus_tag="ACHKUI_003781"
     CDS             2106..2255
                     /locus_tag="ACHKUI_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599895.1"
                     /translation="MIQKVQMMNKDRDRRYGRKRYTTGAMCFMLFVWLGGVLALVATA
                     AVVMQ"
     gene            2436..4061
                     /locus_tag="ACHKUI_003782"
     CDS             2436..4061
                     /locus_tag="ACHKUI_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599896.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNAALATEQIISPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPANMDIRYLSTPFDCACVDLVNDVMRVVSGQNMSAIAGGS
                     LPSTIRWHSKVFSLPERTSFSCLSVKSPTPERVGITVLADDVPVIHLAPGSFSGRVVR
                     LPAATGQNWQMLVSGFGQVERITLSTSMSELPV"
     gene            4058..5326
                     /locus_tag="ACHKUI_003783"
     CDS             4058..5326
                     /locus_tag="ACHKUI_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6599897.1"
                     /translation="MTKKPWRAGKDLSAVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRASGGVSYALNPGIEIDSSLMVVDFPSKPQNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLSDAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAVKGTP
                     AQTQISVQAIIDQIKEEAAKSPVVEELRKEIKNAQGQAVKDAAIETTEVVGNLREETL
                     KTIGGIDTRVTGMNKSTSEELNKVNERITKVDKEGGEAFLAMWSKKTGVEGITAGIGI
                     VAGKDGEGKPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGASITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLS"
     gene            5341..5622
                     /locus_tag="ACHKUI_003784"
     CDS             5341..5622
                     /locus_tag="ACHKUI_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599898.1"
                     /translation="MAEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEG
                     KTLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            5628..6245
                     /locus_tag="ACHKUI_003785"
     CDS             5628..6245
                     /locus_tag="ACHKUI_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599899.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINSSIYD"
     gene            6325..7062
                     /locus_tag="ACHKUI_003786"
     CDS             6325..7062
                     /locus_tag="ACHKUI_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6599900.1"
                     /translation="MKSIMKFFACAVLVMSCLTAQVNAASGDSTVSLGFAHIRFPGLK
                     DFVRDAGVYNRDTFRNVVNVNHFNSSAEYENAIARGHDGTAKSPLGMSIRYRYEITDE
                     LGVIASFTWARSMTNAQAFIDVKPSDPSREVKNPAASARTDIRANYWSLLSGPSWRFN
                     EYLSVYAMAGMGVAKVTTDLKINDNLNHGAGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
     gene            7295..7435
                     /locus_tag="ACHKUI_003787"
     CDS             7295..7435
                     /locus_tag="ACHKUI_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6599901.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            7492..7893
                     /locus_tag="ACHKUI_003788"
     CDS             7492..7893
                     /locus_tag="ACHKUI_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599902.1"
                     /translation="MAPVSGERMDDTILRYMQRVVRNSRNPEFMNEVKDAGLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSKSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            7987..8643
                     /locus_tag="ACHKUI_003789"
     CDS             7987..8643
                     /locus_tag="ACHKUI_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599903.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     KKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVIGQIDTTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGNGGGGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            8646..9092
                     /locus_tag="ACHKUI_003790"
     CDS             8646..9092
                     /locus_tag="ACHKUI_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599904.1"
                     /translation="MEYGKYETLARAGYSGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGKLVSA"
     gene            9102..9353
                     /locus_tag="ACHKUI_003791"
     CDS             9102..9353
                     /locus_tag="ACHKUI_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO6599905.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAMGAAVGGLPGAAIGAVIGGIAGSLF"
     gene            9364..10629
                     /locus_tag="ACHKUI_003792"
     CDS             9364..10629
                     /locus_tag="ACHKUI_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599906.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRLRMQKY
                     NQRRLEYNDMLARDQPVMAALGKAVDAGDRDAAMRLYGQLSEGNPLRLMANDGYVAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKGAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSP
                     QHQQKIQQQKIQQGITSWAGDDPDKQAFAREVASRQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDDSAASASEFIRGLKQNYAQ"
     gene            10698..19064
                     /locus_tag="ACHKUI_003793"
     CDS             10698..19064
                     /locus_tag="ACHKUI_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6599907.1"
                     /translation="MAYSDEQRPEAQFGNQNRNSLNIQLPGETDSYDAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGVAQSVRGAGEMARGLGDAMIQNPVKTGARILNEFSRMGLPG
                     VATVQDIFVGGSKGADEVIDTLPDGKNAVTDTVGKGLKVTGKAVSGGAKASDEWLTGK
                     MSPGAQRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVTKKLGEVTLRKVLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSSASAQGQSAMAAADSVLN
                     ADYSELAKSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLAMRGTAKTVKSGIVRNATEQGTINAAQGGYSRYQENMTLRETA
                     GMDISPWEGVADATIEGAALGAAMGAPFGAVAGYRGKRQAAEETAMREAEVVQQGEAA
                     PQPEPADPVAQHRESMQGMNREQLLEQYADADMAPEGDTSAVHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRPELLEEYRKLSLKEGRTDTEEQQLQAIRDVLRPQQEARPEAQP
                     QPENADDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNRYFTDEKSALG
                     SDVFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADAEPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGR
                     QGEILEGEPVRRGLPSPDEQNATAPVREGLPAPEQRGVDMPHPESLPRMVRDSLPELA
                     QQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQARGAKIEDFGEEIKGAAKHRYA
                     QLAETLGKTLEDRDYFTQPLSKVFPKPDYAKLANEGADADTLAMIALYRSDIPAKTKL
                     NAVKWVESVKSIRTSVAGMLEGKVTAGRLAEWMEGRMPSRYADTWQLLRTLPPSQMDK
                     ASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKAWFAEQ
                     EEKSRAKGDEKTAPSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDARKYI
                     DSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGN
                     YVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGE
                     VAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGR
                     YVDAEYPVRQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAARAFERY
                     VQDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRET
                     DKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRV
                     VKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAM
                     EHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQ
                     PNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGA
                     GASSQSPRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAP
                     DLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVD
                     KNGDPVVSAVHMKATDKRIEINKVASVYGTKGGMNKANSLDKAGLTLYRKEKLSRDNP
                     QYSGLQLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQ
                     VLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIA
                     QADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRL
                     AGIIDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDIDGVVSLPRTGKGFT
                     IGGRESRQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDKDYWQV
                     FTDDKPDTMRAIAERVDPETGETRREVVERPVPMAMMADRYFTTKKDGKTYYIKLHDP
                     RLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAE
                     QGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGG
                     KTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSA
                     YKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGH
                     LNGDGPLPERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHV
                     KERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGG
                     ILGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKP
                     DSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISK
                     TTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGA
                     ERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQAAMVKSVERDM
                     KIAVDRFNREYNKKVGVD"
BASE COUNT         4958 a         4243 c         5711 g         4327 t
ORIGIN      
        1 taatgagtaa aaagtttaca atgacactcc tgtcatcttc tctggccggg cttctggtga
       61 tgagtggtgg ggtgagtgcg caggaagaaa aatacacagt gccgtatgcc acaggtaaca
      121 ataaatgggg gaatacctat gaagttgtaa agacaggcga taatggtaac ttcaaatacg
      181 aagtaaaaga gaagaacaac ggcaagagga cacttttcac atttgattca aatggggatg
      241 taataatcaa tggcagtggc attacgtaca caattcatga cggtgcgctg aacgattttg
      301 ctcaaacagc tgagaaaaaa aagaatggac cgtctcagtc gcacagaatg actgatagcg
      361 ttgttcgtga tgtatataac aaagtataca gtctgcagag aacaaaaatt acaggtttca
      421 gcgtcgaaga tggggagaat gggaaggttt ctctgggtag cgatgcgaaa gcctctggtg
      481 agttctcagt agctgttggt acaggggcca gagcggataa aaaatttgca acagcagtgg
      541 gctcatgggc tgctgcggac ggtaaacaaa gtacagctct gggagtggga gcatatgcct
      601 atgcagatgc ttcaacagcc gccggaacag ctgcttatgt tgatggttct gctatttatg
      661 gtaccgctat aggtaattac gcaaaagtgg atgaaaatgc aactgaaggt gttgcattag
      721 gagccaaagc aaaagtggat aaaaatgcaa cagaaggtac tgcgttaggg gcaaaggcaa
      781 cagtaacaaa taaaaacagt gttgcattag gagctaattc acgcacaacc cgtgacaatg
      841 aggtttatat cggttatgaa gcagagccgg gtaaggctta taaaacccgg gtgcttggtg
      901 gtctgagtga cggtactcgt ccctcagatg ctgcaaccgt tcgtcaggtt gaccgtgtaa
      961 aagacagtgt tgagcaactg gctcaggata cgaatacccg ccttgtagtc gaagcgaaaa
     1021 agtctcgtga atatacagac tccagaacga cagttggtgt gaaccctgac ggaaaactta
     1081 cccgtgcaga aggtgcgact aaaactatcg ccgtcaatga tggtctcgtt gctctgtctg
     1141 gcagaacaga ccgtattgat tatgcagttg gtagcgttga ccgccgggtt actaaaaata
     1201 cacaggctat tcagtccaat actcgccagt tgcaggagca taatgcacgc ctgaacagtc
     1261 agcagcgtca aattcgtgag aatcacgaag agatgaagcg tgcagcagca cagagtgcag
     1321 cgctggcggg gcttttccag ccgtacagtg tggggaaatt caacgccact gctgccctgg
     1381 gggggtacag tgataaacag gctgttgccg tgggtgttgg ttaccgtttc aacgagcaga
     1441 ccgcagcgaa agcgggtatt gcagcgagtg atggcgatgt gtcctacaac atgggcgtta
     1501 acttcgagtt ttaattgctg aaagtgacat gaagtatggc ggcaggcgca ggactctggt
     1561 ctgccgtttt tcatggggca taaaaaacag gaaggtgctg cgatgaaggt gattctggct
     1621 accaggaacc gctatctgga gtatggtctg caggcgttac tgaaggggca cagtgtgata
     1681 ctggcgaggg agtttttcct gccggagaac cgccgttata tcccggactt tgacgaatcc
     1741 tggctgataa tcagtgatgg cctgctgggc aggctgatgc gctgtatgtt ccaggggcgt
     1801 cattttctgc aactggatgc ggagttgctc cgtgatggtg agcagataag tgatgccata
     1861 cacaacggcg tctggacgta taacagtgct gcccgcccgc tgacgatgtc agagatggtg
     1921 gtgatgttcg gttatgtcta ccgccagtcg agaccctgcc gccttgccag tgagatgggt
     1981 attaacacaa agacggtgaa tacctttctg tatacgggaa tggcgaaaaa cgggctgtat
     2041 ggtgtgagcg tcagacggct tgttggtgcc tgatggcggt cctgccggat gcgcggcatc
     2101 ttctgatgat tcagaaggtg cagatgatga acaaagacag agaccggaga tacgggagaa
     2161 aacgttacac cacaggcgca atgtgcttca tgctgtttgt gtggcttggt ggtgtgctgg
     2221 ctcttgttgc tacagctgcg gtggtaatgc agtaaaaaaa ggggagcaac atgctccccg
     2281 accagaagaa agacgtttga taacaattag tgagttgtta cgtcttgtcc agaatatcat
     2341 agcaacactc tgttgcagtg ataccgatcg cgattttagc gaattccatc ataaatcccc
     2401 tgatttttaa gcctgaagca gtcaaaggaa tttctatgcc ctatatcgat atcaccacga
     2461 tgcgtgggat gatgccgcgc gttgtgacat ccatgctgcc cgagcattcc gctgtactgg
     2521 cggaggactg ccatttccgg tttggtgtta ttacaccaga acgtcagata tccggggttg
     2581 agaaaacatt cacaattaag ccaaaaacaa tttttcatta ccgtgacgat ttctggtttg
     2641 catggccgga tgtggtggat gtgatccgca gtccgatcgc tcaggacccc cacgggcgta
     2701 tttactacac tgacgggcgt tttcctaaag tgacggatgc gaccattgcc acaaaagggg
     2761 acgggaatca cccgacatca tcgtatcgtc tggggatccc cgcgccgacg acagctcctg
     2821 tctgtactgt tcagcagggc ggtgatgttt ctgacgataa cccgaatgat gatgaaaccc
     2881 ggttttatac ggaaaccttt gtctcagatt atggtgaaga aggtccgcca ggtccggcgt
     2941 ctctggaggt aacactccgt actccgggaa ctgcggtaca actgacgctg gctccggtgc
     3001 cattgcagaa tgccagtatt aaacgtcgtc gtatttatcg ttctgcatca ggtggagggg
     3061 aggcggattt tttacttgtg gctgaactgg atgcatccgt gctcagttac acggacaaaa
     3121 taccggcgaa aaaccttgga ccttcgctgg cgacatggga ttacctgccg ccgccagaga
     3181 atatgacagg cctttgcctg atggctaacg gtattgccgc cgggtttgcc ggtaatgaag
     3241 tgatgttttc ggaagcgtat ctgccgtatg catggccgga agtgaatcgt cacacgacgg
     3301 cagaagatat tgtggctatc tgtccgctcg gaacatcact ggtggtggcg acaaagggtg
     3361 agccttattt gttcagtggg gtctcaccgt ccacaatttc tggttccaga atcccttcaa
     3421 tgcaggcgtg tctgagcagg cggagtatgg tcgcgatgga gggctttgtg ctgtatgcag
     3481 gaacaaatgg cctggtgtct gttgatgcaa acggtaatgc cgctctggcg acggaacaga
     3541 ttatttcacc ggaacagtgg cagagtcagt ttaatccggc ctccattgtg gcttatccct
     3601 ggcgtggtga atacattgcc tgttacacga aaccggatgg taagcaggat gtgtttgtct
     3661 tcagcccggc gaacatggat atccggtacc tcagtacgcc gtttgactgc gcatgtgttg
     3721 acctggttaa cgatgttatg cgggtggtat caggacaaaa catgtctgcg attgccggag
     3781 ggagtttgcc gtcgacaatc agatggcatt caaaggtatt ttcccttccg gaaagaacct
     3841 ctttttcctg tctcagtgtg aagtctccga cgccagagcg ggtgggaatt actgtgctgg
     3901 ctgatgatgt gccggtgatt caccttgcac caggaagctt ttcaggacgc gttgtgaggt
     3961 tacctgctgc aaccgggcaa aactggcaga tgctggtttc tggttttggt caggttgaac
     4021 gtatcacact cagtacatcg atgtcggagc tgccggtatg acaaaaaaac cgtggcgcgc
     4081 aggaaaggat ttatctgcgg ttgtggaaaa tatggagatt ggcacagggc agcgtggaga
     4141 tggtcgtcat gcttttgtaa cccgtgaaga gctggttggt ctgaagcttg cccgtcgccg
     4201 ggcttcagga ggtgtctcat acgccctgaa tccggggatc gagattgaca gtagcctgat
     4261 ggtcgttgat ttcccctcga agccgcagaa ttttaaggct accgggggat ttggttcggt
     4321 gcttcttgaa tgggatatgc cgaattatcg cggtcattca ctgactgaaa tctggcgggg
     4381 tacggaggat gacctttcgg atgcggtgct ggttgccacg acgccggggc aggtttacgg
     4441 tgatccggtt gatccgggct ggtcgggatt ctactggata cgctttgtta atgcagcggg
     4501 agtgaaaggg ccatggaacg cggtaaaagg cactcccgca caaacccaga taagcgttca
     4561 ggccattatt gaccagatca aggaggaggc tgcaaagtca ccggtggtgg aagagctgcg
     4621 taaggaaata aagaatgctc aggggcaggc agtaaaggac gcggcaatcg agacgacgga
     4681 agttgtgggt aatctcaggg aagaaacact gaaaactatc ggtggtattg atacccgtgt
     4741 tacggggatg aataagtcaa ccagtgaaga gcttaataag gtaaatgagc gcatcactaa
     4801 ggtggataaa gaaggcggtg aagctttcct ggccatgtgg tcaaaaaaga ccggcgttga
     4861 gggaattact gcggggatcg gaattgttgc aggaaaagat ggtgaaggga agccggtaag
     4921 ccaggttgca atttcggcat cgcagttgtt tgtctttgac ccgaacaacc cggataacac
     4981 agcctatccc tttgcggtgt caggtggaaa agttgttatt cccaaagcga tgatttatga
     5041 cgcggtgatt gaaacactgg tgtcgcggaa ggttgtggca gatgaagtaa aagccggggc
     5101 aagtatcacg tcgcctgtta tccgtagtgc tgttattcag aacgggaact ttcaggttga
     5161 ttctcagggt aacctgaata ttggtggcct ttttagtgtt acgtcgcagg ggcaactgac
     5221 aattcgttac tctaatcaga atgtggggct ggtgatccgc aatgataaaa ttgaagttta
     5281 tgaccagaac ggacgcctgg ctgttcgcat aggcaggtta agctgagccg gaggctgaca
     5341 ttggcagaat atggttttgc aatatacaac agaaataatg ttaatgttac gggcgtgctt
     5401 actcccatat ttttcctgga caggtttacg gcggagtcgg gatcaaaaac gtatacgaat
     5461 aaaccagaag ggaaaacatt acaggcagtc tgttcattat ttccgtggaa taatgtattt
     5521 aaggaccgga aagtaccgaa gataaccatt aatggcaata cggtgacatg gtcgaatctt
     5581 gagcagggga tggggtcata tatttataca ttctgggggt gagttgtatg tatggtttaa
     5641 gcattacgaa accagatggc agcttgtgga taagcccggg gtttacgcct cagtgtctga
     5701 tcaataaggg aactattccg gcgactgaga aggctttttt taaaacgtca atcccgtcag
     5761 gaaaaagttg ttttttcttt atcagaacag agaaaaaggc cgatgttatg tacacgcatg
     5821 aacaaattga tggatatcat gccttaaggc ttcatcaaat tgtcagggga acgaatcccg
     5881 gtgttacgac tgtttatgct tttgcgaata tggttactca accttctgag tatggtatcg
     5941 ccatgtataa cccgtcgggt gagatgattt accatggcga aatgatgctg cttgacgcga
     6001 agttaatacc agttgatatc aagtttgaga aggaccttgg atatccatgc gcaattatgc
     6061 ctgcactggt tggatattat aactggaaac gaacacctta tgaccgaccg atttacacca
     6121 catccactgg tgctacagga aataaaatat attcctgtga gcattattcc ggtggtgcaa
     6181 catgggatat ccgaaagccg tatatagata aagttctggt tattaattcc tcaatatatg
     6241 attagcgtaa gaaaaacttt aatagtgtaa aacgaaagcg ttcctttcag acatcgcatc
     6301 ctttaattca gagaaatact taaaatgaaa agtatcatga aattttttgc gtgtgcagtt
     6361 ctggtgatga gttgcttaac tgcacaggta aatgcagcat caggcgatag tacggtatct
     6421 ctcgggtttg cacacatacg cttccccggg ctgaaggatt ttgttagaga cgcgggtgtt
     6481 tataaccggg atacgttcag gaatgttgta aacgtaaacc attttaattc atcagctgaa
     6541 tacgaaaatg ctattgcccg ggggcatgac ggcacagcca aaagccccct ggggatgagc
     6601 atcaggtatc gttacgagat aactgatgaa ctgggtgtta tagcctcttt tacatgggcg
     6661 cgctccatga ctaatgcgca ggcatttatt gatgttaagc cttccgatcc atcacgagag
     6721 gtaaaaaacc cggcagcttc agcgagaact gatatcaggg ctaattactg gagtctacta
     6781 tccggtcctt cgtggcggtt taacgaatac ctcagtgtgt atgcaatggc tggcatgggg
     6841 gttgcaaaag tcaccactga cttgaaaatt aacgacaatc ttaatcatgg tgccggtagt
     6901 ttttctgaaa gtaacagcac caaaaaaacg tctattgcat ggtctgtcgg cgcgcaattt
     6961 aacttcaatg aaagtgttac tatggacgtc acttatgaaa gctcaggctc tggcgactgg
     7021 aaaactgatg gtttatttgc aggcattggc ctgaaattct gagtaatacc cgacaacatg
     7081 tcataacccg ctgtgatggc gggttttttg ttgcccgtgc agggcaaaaa tcgtagatta
     7141 tgcgcgggtg cctttcggct gatggctgga gggagaacct gaaggcctga tgtggaaagg
     7201 ccccgagtca aacattttgt ttaacccgag gccctaacca tcatacctta agcaagtaga
     7261 aggttagcgc ctctccactc aggaggcaag cgctatgtcg caaaaatcgc tcatcaccgt
     7321 cacaatttgt atgacggtaa tcttcaccat ctggatgtta cacggctcgt tgtgtgagtt
     7381 ccggctgaat ttgtggggag cggagtttgc ggcgttctta cagtgtaagc agtaggaaaa
     7441 ccgcgacggg gacgaaagtc cccgtcaact ggttgctgag gttcagccga tatggcaccc
     7501 gtttctggtg agagaatgga cgacacaatt ctccggtata tgcagcgtgt tgtgagaaat
     7561 tcccgcaacc ctgaatttat gaatgaagtt aaagacgccg gccttaaaaa gcaggcgttt
     7621 tgctttgagg caccggatgg ttttctggtg ctgcgttctg tgctcagtga cgatggtatc
     7681 ccttatgttc tggtgttact gggcgtgtgt acggggagta agagtgttga gcgttatctg
     7741 ccggaggtga agacattaac ccgcctggct ggcggacgct gggccgaatt tcatacggca
     7801 agacgaggat ttatccggct gggaaaacgt ctgggctttg agcgaatgcc ggatgatgag
     7861 gatggcttca tggtgttcag gatagcagtc tgactgccac tgtattcatc atcgagcgta
     7921 aacccacatt gcaattcaca ttctgaccct gctccggcag ggttttttgt tatctgagga
     7981 gccattatgg gcggaagtaa aggtggtggt gataccaaag taaaaccaac agcagcgcaa
     8041 atagcacagg aagaggtggc ctggaaaggg tggcaggatt acaaaaatat tctccgtccg
     8101 gcagaagata acttcatgaa aaaggtcgat gaccttaaca gtgagcagca gtacgaaaat
     8161 atcgcaggca caacaaatct tgggtatcag aaacagtttg gtgaagcgcg aagggagctg
     8221 gcgggtaatc ttgctcagtc cggcgttgac ccatccagtg gtcgttttaa tgcagtaatg
     8281 gatgcaaatc aaagcgacca ggtgatcggg cagattgaca cgaccacacg ggggcaggta
     8341 tcgcaggcag ataaatatat tgccgggcta caggatgttg cggctctcgg ttccggtcag
     8401 aaggcagatg cgttacagag ttttaactcc ctggctgaca gcagtctggc aaaagccaaa
     8461 tcggatgcac aggcggcgtt tacgaaacaa caggggcgag cttctcttgt tggcgctggt
     8521 ctgggagctg taggtgcgta cgcgatgcat aaggctggcg gtaacggagg tggtggcggc
     8581 gctaaaacgc caggcaccgg cgctaatgct attcagcatc aggctcagaa ctggagactg
     8641 taattatgga gtatggcaaa tacgaaactc tcgcaagggc tggttattca ggcgcagccc
     8701 gcccacaggg tgactggcag acgtcagcag cgctgacacg ccaacaatac gacgactggc
     8761 gaaccagata tttgccccgc gtggcaaggc tggctgacct tggcgagaac aacagcctga
     8821 tgaatgcgca gcttgcccgg gtgggcggcc ttgccacttc cagtctccgt acagcgcaga
     8881 tggcgcagga taaccagatg gcgagatacg gggtaagccg cccggataat cccaacagta
     8941 acacactggg tttgcgtaat gctctggcaa ttgctggcgc gaaaaatggt atccgtgaag
     9001 cagagcagga tcgccagatg aacatactga cgggggcttc tgcacctgca agacaacaac
     9061 tgagtgttgg cggaaaactg gtgtcagcgt aagggggcaa tatgggatac ggtttactgg
     9121 atattgcgaa tcagtcgcgg cgtgaggcat tacagggaat aagtgatgcc gacagacgac
     9181 gtgaagaaat tgaggccgcg aacaaacaga tggcggcgca acagaaagcg cagaacaaac
     9241 agaatatcgg cacgggaatt ggtacgggtg cagctatggg cgctgctgtt ggtggtctcc
     9301 ccggtgcggc tattggtgct gtcataggtg gtattgctgg ttctttgttt taaggagtgg
     9361 tgaatgagcg gatttgcaca gggtttactt gccggattca gcacggttga ccaggcaatg
     9421 acccgtcgta aggaacttgg tttgcgagaa gcacagcttg ctcaacaaca gaaaaataac
     9481 gagcgcgatt ttgaatttgc acagtcgcag tttgaacata ataaagacgt tgatcagcgg
     9541 aactttgatt acagagccaa agtcgacgac cgtaattatg cactgcagga aagggagttt
     9601 aacgccaacc agaattaccg gaatgcgtcg ctgggtatgg agcagcagcg actccggatg
     9661 cagaaataca accagcgacg gcttgagtat aacgatatgc tggcgcgcga tcagcctgtg
     9721 atggcggcgc ttggaaaggc tgtggatgct ggcgatcggg atgcagcaat gcgtctttac
     9781 ggccagttgt cagagggtaa tccgctgagg ctgatggcga atgatggcta tgtagcgaaa
     9841 gcgggtcagg ccgtgaacaa cctgcaaaaa atctttgatg acaagccgga cagggcgatc
     9901 gcttcactca ataccccgga aaatctcgat gtgctttccg gcgtgtttgc cccggaacta
     9961 caacagcgta ttggcatgcc tgattcaacc ggggaaaaaa cgataaaagg ggccaggatt
    10021 ggcagtatcg taccggcgca gcaggaaggg tacgtactta ttggccttga tctcacatac
    10081 agcgatggct ccaccgcgca taaacctgta acagaatacg gcagtgcgca ccctgatgat
    10141 caaaccgtgc tggcgatacc cgttgataag gctatcgctc aggtcaggga tcgcagcaaa
    10201 tttgcagaga tatcgaaaaa ttatggttat tttatgccga agcagcaggg actttctctg
    10261 aaagagcttc agaagggggc cagcaacgta gcggcggacg cgatcaagaa tggcggtaat
    10321 gcacaggctg cggtggatga atattatgct gcaactggtt caccgcaaca tcagcagaaa
    10381 attcagcagc aaaaaattca acaagggatt accagttggg cgggtgatga tccggataag
    10441 caggcattcg ccagagaagt tgcttcccgt cagccagaaa tgctggagcc tcagaatcag
    10501 aaattgctgg agaacgggta tgcgaatttt ctccgtattc aaaaggccag gggggaacag
    10561 gccagagatg atagtgcggc ctctgcatct gagtttatcc gtggtctgaa acagaattac
    10621 gcgcagtaat tcaccattcc cgttaatact atttcctgat acccggtcat tgtgccgggt
    10681 ttttttatgg agtctgtatg gcttattccg atgaacagcg ccctgaagcg caatttggca
    10741 accagaaccg caacagcctg aacattcagc tgccgggcga gactgacagc tatgatgcgt
    10801 ttttctccga tcccaatcgc tggaaggaca acagcacgtc gttcagcctg ggcgatgtat
    10861 tgccaactat ggggaaaggt gtcgcccagt ccgtccgggg ggcaggggaa atggcccgtg
    10921 gactcggtga tgcgatgatt cagaacccgg taaaaacagg ggcgcgtatt ttaaatgagt
    10981 tcagccgtat ggggctgccg ggagtcgcaa ctgttcagga tatttttgtc ggtggcagca
    11041 agggggcgga tgaggtcatc gacactctgc ctgatggaaa aaatgcggtt actgatactg
    11101 tcggtaaagg tctgaaggtc acaggcaagg cagtcagtgg cggcgctaaa gcctcggatg
    11161 aatggctgac tggcaagatg tcgccggggg cgcagcgggc gctgaataca ccgatgactg
    11221 aaggatatga tgattctgcg gtctgggtgg cgaagggcgt aaacctgatt ggtgcccttg
    11281 tacctgatat ggttgcaggc ggtgtgacta aaaagctggg tgaagtcact ctgcgaaaag
    11341 tgctgaccgc cgggctggag aaaaaataca ttgcggcagg gatgcagccg gaaagagcta
    11401 ccgcactggc agcagaagct gtcgataaaa aaatgccgga tttattccag gcaggcctga
    11461 tcacccattc ctcagccagc gcacaggggc agagtgcaat ggcagcagca gactctgttc
    11521 ttaacgcgga ttactctgag ctggcgaagt ccccgaaatt tcagcagacg tttttgtcaa
    11581 ttgatgcaga tccgcagcac gcacagctta ctgatcgcca gaaaatggat ctggcaaaag
    11641 agcgtgttgc cgatgaggtg cgcgcgcagc tggcaaccga tcctgaattg ctggctgtga
    11701 atgccatggc ggcaaaactg ggcgatgctc agttgtttaa tctggcgatg cggggcacgg
    11761 cgaagaccgt taaaagcggc attgtcagaa atgccacgga acaggggacg attaatgcgg
    11821 cgcagggcgg ctattcgcgt taccaggaaa atatgacgtt gcgtgagact gccggaatgg
    11881 atatatcacc atgggagggg gtagctgacg caacgatcga aggagccgct cttggtgcag
    11941 cgatgggcgc accgttcggt gcggttgccg gatatcgtgg aaaacgtcag gccgcagaag
    12001 aaaccgccat gcgtgaggct gaagtcgtac agcagggtga agcagccccg caacccgaac
    12061 cggctgatcc ggtggcacag catcgcgaat ccatgcaggg catgaatcgc gagcaacttc
    12121 tggaacagta tgctgatgcg gatatggcac ctgagggaga cacgtctgcc gttcatcgcc
    12181 gggaagccgc cagccagctg ttaaatgaac tggatgaaca ggcgaagcga caggcggtga
    12241 tggatgagct gaaggcgaag ccgcgccctg aactgcttga ggaataccga aaactcagcc
    12301 tgaaggaagg gcgtactgat actgaagagc agcagttaca ggctatccgt gatgtgcttc
    12361 gccctcagca ggaggccaga ccggaggcac agccacagcc ggaaaatgcg gatgatggcg
    12421 acgggagtat ttacccgacg gtgcggttcc gcgatccgga tgaagttcgt attgaaatta
    12481 acgggagtgg tgcgtccaga ccagcagaac gcattgaaaa ggtgcgtccg gacaaccgtt
    12541 atttcacgga tgaaaaaagt gctctgggga gcgacgtttt ccgcaatgcc agcgccaccg
    12601 gcctgaaacc gtccgtagtg aagaaaggcg agaatcagta tgccgttgaa atggataatc
    12661 ctgcgttctc tgaagatgtg gcaacggaga ccattaacac cctggctgac ggagagcgta
    12721 ttgctgatgc tgaaccgatg gagcagcccg cgttcatgcg tgacccgcga ttccgtggtt
    12781 tcacggggga tgatacagaa gtgcaggccc gccttgcccg tggcaacgcg ccgacggcag
    12841 aggagcttgt acgttcacag atggctgaag gtgatgccgg tccgacagca caggagttaa
    12901 ctgagcgtcc acgccttccc gctcccggcg atattcatcc cggacaggga tatccgttac
    12961 caggagaagt ggcgcgtacg ccggatgaaa atcaggccgg acgtggtggt cgttttacca
    13021 caaccggtga ggtgaagggc cagagtttcc agaaaggacg tgcgcaggca ccggaaaatg
    13081 ccgctggtcg ccagggggaa atactggagg gcgaaccggt tcgtcgtggt ctgccatcac
    13141 cggatgagca gaacgccaca gcaccagtgc gtgaaggact tcctgcacct gaacagcgtg
    13201 gggttgatat gccacatcct gaatcactcc ctcgtatggt tcgtgactcc cttccggaac
    13261 tggcacagca ggcagaagcg cgcagacaga ccggggataa tcgccagacc ataaccgatg
    13321 ttccggatac tgaggtgccg gtgccggtag ataaaccgag cactcaccag caggcgcgtg
    13381 gtgcaaaaat tgaagacttt ggcgaggaga ttaagggcgc ggcaaaacac cgttatgcac
    13441 agcttgccga aacactgggt aaaacgctgg aagacaggga ttatttcacg cagccgctga
    13501 gcaaagtgtt cccgaaaccg gactatgcaa aactggcgaa cgaaggggcc gacgctgaca
    13561 cactggcaat gattgcgctg taccgcagtg atattcccgc gaaaacgaaa ctcaatgcgg
    13621 tgaagtgggt ggagagtgta aagagcatcc gcacaagcgt ggcgggaatg ctggaaggaa
    13681 aagtgactgc cggacggctt gctgagtgga tggagggaag aatgccttcc cgttatgcgg
    13741 atacctggca actgttacgc acgctgccac cttcacagat ggacaaggct tctgcttatc
    13801 gggtggtatc cggtgtgtat caggcggccg gagggaagcg ttacgatccg ccacagaaac
    13861 tttattcact gcgcaataag gacaataagg ggagtaacct ctttttctcg gaaagcaggg
    13921 atgaattact gacaaaggcg aaagcctggt ttgcagagca ggaggaaaag tcacgggcga
    13981 aaggtgatga aaaaacagca ccgtcaccag atgacaaaat cagctttgac gtttaccgga
    14041 atacccgtag tggcgatatt tttatcgctt acggcaaaaa caaaatgcgg gtgagaggtg
    14101 gttttaagtc agccagtgat gcgcgtaagt acattgattc acatcgtgat gagcttgttc
    14161 gtcatgtgaa ggagatgcgg gagatttcgc gtgaggagca gcgtaacgcc actaaccgcg
    14221 accgcaccgg accagaacgc cgcaaagggg atgtttcgcc ggagcagttc agtgatgcgt
    14281 ttggtttccg tggtgtgcag tttggtaatt atgtggaagg tccgcgtcgt caggctgatt
    14341 tgaaccgggc ttatgactcg ctgcatgacc tggctgacgt gctgaacgtt ccgacaaaag
    14401 cgctttccct gaatggtcgt cttgggcttg cttttggtgc ccgtggtaag ggtaaggcgg
    14461 cggcacacta tgagccgggt gaggtggcaa tcaacctgac aaaaggtaac ggaccggggg
    14521 cgctggccca cgaatggttc cattcactgg ataattattt tggtcgttat gatgtttcca
    14581 ctgacgggaa gatcacgtca ggtggcgact atatgacgga agcacaacgt gccgggcgcg
    14641 tatttaaaga cggcagatat gttgatgcgg aatatccggt acgtcaggag gtttacgacg
    14701 cgtttaaagg cgtgatgaaa gcgatcaaca gcagtgacat gccgcgtcgt tcagcgcttc
    14761 tcgatgaggt gcgctcaaaa ccgtactggt caacggatgt tgaaatggcg gcgcgtgcct
    14821 ttgagcgtta tgttcaggat aaagcgcgta cggctggcgt ggagaatgat tatctggtca
    14881 atatccgtaa ggcacctgag cacaacacag ataacacctg ggcttatccg acgaatgcgg
    14941 aactggatgg cggtattcgt gatgcattcg atcacctgtt cagtacgctg aaaacccgtg
    15001 agacggacaa gggcgttgcg ttttattccc gtaagggcgt gacccgcaca cctgaaggca
    15061 atctcatttc ggatgttaac cgcagtgcgg aagccaaagg cagcccggtc ccgcaggttg
    15121 aagcggttgc ccgtggcgtg atgagtggca ttaaggacag tgacctgaag gtccgcgtgg
    15181 tgaagtcaca gaaagaggct gaagcgctgg cgggtgaatc gttcgacggt tacggcaggg
    15241 tgcatgcgtt ctatcgtccg gataaacgtg aaattgtcct ggtggcggat aacattcctg
    15301 acgggcggac cgttcgcgag aagctgcgtc acgagattat ccaccatgca atggagcatg
    15361 tagtcacgcc agcggaatat cagacgatta ttaagaccgt gctgaaaacc cgtgacagtg
    15421 ataacgccac catccgtgaa gcctggcgta aggttgatgc gtcttatggt aaggaatcac
    15481 cggaagtgca ggcgggtgaa tttctggcac atatggcgga gaaacagccg aataaatttg
    15541 tggcggcgtg ggagcgtgtt gttgccctgg tcaaaggggt actgcgtcgc acggggttac
    15601 tgaagccgac agagctgaac gatatcagac ttgttcgcga aactattcgc acgttaggcc
    15661 agcgtgtgcg ggaaggttac acgccgcgtg aggatggcgc gggcgcatcg tctcagtccc
    15721 cccgtagtgg taaacctgat ccgttcaaag tgccggaagg tgagggcgag cgttatcgtg
    15781 atgaccttgc cagaatgatg aagtcgttac gttctggtgc aatgactgca aacatcgggc
    15841 gtacgccgcc ggtattgcgc caccttggcg caccagattt gccgctggtt atctcccgcg
    15901 acactgtgcg gaaggcaacc aatggagtga aacatgtggt gccaatggat gttatcgaga
    15961 gacttcctga gctgatgcac gatccggatg caatttaccg ttccgcgaca gaaagaaatg
    16021 cggttgtgat gctgcttgat gccgtggata aaaatggtga tccggtggtg tcggcagtgc
    16081 acatgaaggc aacagataaa cgaatagaaa ttaacaaggt ggcttctgta tatggaacaa
    16141 aaggggggat gaacaaagct aatagcctgg ataaagcagg tttgacgctt taccggaagg
    16201 aaaaattaag ccgcgataac cctcagtaca gtgggcttca attgcccaaa gaggagcgtt
    16261 cttatcgcgg ctctgtagat aaaatactct atcctgaaga tattcgcaag gggccgtatt
    16321 actcccgtac cagcagtctg acaccggaag agacaattgc atcgcgtttt gtgcgtcaga
    16381 tgcaggataa attccaggtg ctgaaagctg ttcaggagaa catccgtaaa actggcggaa
    16441 aaatagacga cagtaacaac gcttatatgg cggaagaact cttccacggg aaggcggaaa
    16501 acgacctgaa cgtgatgaag gagcgctacg ttcagccact ggctaaatta ctggcggact
    16561 acaaaattgc gcaggccgat ctggatgagt acctctacgc ccgtcacgcg ccggaacgta
    16621 acgcgcatat cgcgaaaatc aacccgaaaa tgccggacgg cggttcgggg atgaccaacg
    16681 cggaagcggc ggaaatcatg cagcgtgtac gtaacagtgg caaacaggca cagtatgacc
    16741 gtctggcagg gattattgac gatatgctgg cccgtcgccg tgagcttatc cgtgaggccg
    16801 gacttgaaga gagtggtgtg gtggatgcct ggcagaacgc ctaccgttac tacgttcccc
    16861 tgaaaggtca ggatattgac ggtgtggtgt cactgccccg tacaggtaag ggcttcacta
    16921 tcggcggacg tgaaagcagg caggccatgg ggcgtgcatc ccgtgcacag tctccgtcca
    16981 ctcaggcgat acaggatttg agcgaatcgc tgatccgcaa tcgcaaaaac gaagtgggta
    17041 acgccttcct gaaactggtg caggataatc ccgataagga ttactggcag gtattcactg
    17101 atgacaaacc ggacaccatg cgggcgattg cagagcgtgt tgatccggaa accggcgaaa
    17161 cccgtcgcga agttgttgaa cgtccggtgc ctatggcaat gatggcggat cgctacttca
    17221 ccaccaaaaa ggacggcaaa acttactaca tcaaactcca tgacccgcgc ctgatgcgtg
    17281 cgatgaagaa catggggccg gaaaccagta acgccgtaat ccgtacgctg gggaaagtta
    17341 accgtttcct ggcaacggtg aacacgtcgt ataacccgga atttctggtc agtaacttca
    17401 tccgtgacgt gcagacggcg gtgatgaacc tgaaggctga gcagggaagg agcgacggta
    17461 aactgaaagg gctggataac ttatccgccc tggctgtggt gaaagacagc cggtctgcca
    17521 tgtctgccgt atacgccagt ctgcgtggta aaaccctcac gggcaacggt gcgcagtggc
    17581 agaaggtgtg gaaagagttt gttgaggacg gaggtaaaac cggctggttt aacatgggtg
    17641 accttgaagg ccagcagaag gaaatggatc gccttgtatc gctggcgaag ggaggatgga
    17701 aaggccagag tatcggtgca tggaattcgt tccttaacct tgtcgaggat gccaacgggg
    17761 cggttgaaaa cgctctgcgt ctttctgcct ataaacacgc ccgtgatgct ggtttgtcac
    17821 gccagcaggc ggcgtctctt gccaaaaaca tgacggtgaa ctttaaccgt cgtggtgagc
    17881 agggagcgct gatgaactcg ctgtatatgt ttgccaacgc cagcattcag gggaccgcaa
    17941 acctggtgag aacgctcgga catcttaatg gcgacgggcc gttaccggag cgccttcgct
    18001 ggaagaatct caatgtaccg cagaaaatcg cgcttgcagc tgtgggagcg ggttatctgc
    18061 ttggctcgct taaccgcagt gttgcggggg aggatgatga cggggttaac tggtatgaca
    18121 aggtgccgtc tcatgtgaaa gagcgtaacc tcgtcattat gaaatcggtg ttcgggggca
    18181 aggccggaga gtactggagt attcctctgc cttacgggta caacgttttc ttcctgctcg
    18241 ggcatactgc tgaaggtgtg gcagcgggcg acctgactgc ttcacgtgct gccggtaatg
    18301 ttgtcggtgg tatcctgggg gcattcagtc ctgtgggcag tgaaacgtcg gaaacactgt
    18361 ccggggcatt gctgaaaaat gcagcgccga ccattctgcg tccgtttgcg aaccttgcca
    18421 tgaatgaaaa cttcatgggg gcgcagattt accaggagaa catgccattt ggcacaccga
    18481 aaccggacag ccagctgggc agacgttcaa cgcctgaggc gtacaaggcg tttgcatcct
    18541 ggctgaatgc gttctcaggt ggtagccagt atcgtcccgg cgcggtggat atcacaccgg
    18601 aatcgctgaa attctggatt gactatatct ccggagggac agggcgcttc atttccaaaa
    18661 ccacagatgc ggcggtgaaa tcgctgaacg gtattgatat accggagcag caggtgccct
    18721 tcctggggaa aatttcgggt gaggtgatgc cgtatgctga ccagcagaag atgtatgacc
    18781 ggatgacgga ggttgcgcag taccacgcag agctgaagag tctgaccggt gcagaaagaa
    18841 cggcattcat tgacgagaac aacggaaaat tgtcgatgaa cgggcttatg caggataccc
    18901 ggaagagact gaaggatttg cgtaaacagc gtgatgccat ttacgccgac agtactctca
    18961 gtctggcgca gcaggcggcg atggtgaaat cggtagagcg ggatatgaaa attgccgtgg
    19021 atcggtttaa ccgcgagtac aacaaaaaag tgggagtgga ttaacagaaa tggccccgta
    19081 cggaagtgcg gggctgatat aaagtcacgc atggaaatat tgtagatgaa ctgaacagtt
    19141 aaattcggat aatgttgtga tgttgatcag aaattacagt gtaagcagat gtgaaacagc
    19201 aagtctgatt gagggtaagt tttttctgat gtctgttcc
//
LOCUS       DAZFRE010000092        18945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFRE010000092 DAZFRE010000000
VERSION     DAZFRE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.092"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            441..875
                     /locus_tag="ACHKUI_003794"
     CDS             441..875
                     /locus_tag="ACHKUI_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO6599908.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            968..1234
                     /locus_tag="ACHKUI_003795"
     CDS             968..1234
                     /locus_tag="ACHKUI_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599909.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1411..1800
                     /locus_tag="ACHKUI_003796"
     CDS             1411..1800
                     /locus_tag="ACHKUI_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO6599910.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            1797..2711
                     /locus_tag="ACHKUI_003797"
     CDS             1797..2711
                     /locus_tag="ACHKUI_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6599911.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPKVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            2711..2956
                     /locus_tag="ACHKUI_003798"
     CDS             2711..2956
                     /locus_tag="ACHKUI_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599912.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(2987..3238)
                     /locus_tag="ACHKUI_003799"
     CDS             complement(2987..3238)
                     /locus_tag="ACHKUI_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6599913.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHTWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            3819..4667
                     /locus_tag="ACHKUI_003800"
     CDS             3819..4667
                     /locus_tag="ACHKUI_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6599914.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKQEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            complement(4753..5088)
                     /locus_tag="ACHKUI_003801"
     CDS             complement(4753..5088)
                     /locus_tag="ACHKUI_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO6599915.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            5321..5653
                     /gene="mobA"
                     /locus_tag="ACHKUI_003802"
     CDS             5321..5653
                     /gene="mobA"
                     /locus_tag="ACHKUI_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO6599916.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            5665..8376
                     /locus_tag="ACHKUI_003803"
     CDS             5665..8376
                     /locus_tag="ACHKUI_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO6599917.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAELQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            8444..8875
                     /locus_tag="ACHKUI_003804"
     CDS             8444..8875
                     /locus_tag="ACHKUI_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO6599918.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            9225..9629
                     /locus_tag="ACHKUI_003805"
                     /pseudo
     CDS             9225..9629
                     /locus_tag="ACHKUI_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(9736..10572)
                     /locus_tag="ACHKUI_003806"
     CDS             complement(9736..10572)
                     /locus_tag="ACHKUI_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO6599919.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(10572..10847)
                     /locus_tag="ACHKUI_003807"
                     /pseudo
     CDS             complement(10572..10847)
                     /locus_tag="ACHKUI_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(10922..11395)
                     /gene="dfrA14"
                     /locus_tag="ACHKUI_003808"
     CDS             complement(10922..11395)
                     /gene="dfrA14"
                     /locus_tag="ACHKUI_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFO6599920.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(11416..11943)
                     /locus_tag="ACHKUI_003809"
                     /pseudo
     CDS             complement(11416..11943)
                     /locus_tag="ACHKUI_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(12004..12819)
                     /gene="sul2"
                     /locus_tag="ACHKUI_003810"
     CDS             complement(12004..12819)
                     /gene="sul2"
                     /locus_tag="ACHKUI_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO6599921.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(13683..15986)
                     /gene="trbC"
                     /locus_tag="ACHKUI_003811"
     CDS             complement(13683..15986)
                     /gene="trbC"
                     /locus_tag="ACHKUI_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO6599922.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            complement(15967..17091)
                     /locus_tag="ACHKUI_003812"
     CDS             complement(15967..17091)
                     /locus_tag="ACHKUI_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO6599923.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(17088..18413)
                     /gene="trbA"
                     /locus_tag="ACHKUI_003813"
     CDS             complement(17088..18413)
                     /gene="trbA"
                     /locus_tag="ACHKUI_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO6599924.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            18751..18897
                     /locus_tag="ACHKUI_003814"
     CDS             18751..18897
                     /locus_tag="ACHKUI_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599925.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
BASE COUNT         4463 a         4661 c         5305 g         4516 t
ORIGIN      
        1 cgctacggtc cgatgtctga aaaaccaggc ttgcagcgac cgttccggtc gcgccgcctc
       61 tgtcgcgccg tgacctgcgc tccctgcaca cctgacggtg tgtaaggaaa ctgcgcctga
      121 tggcgcagtt gtctgccctt ccgccgcccc ccctgcggcc tgtttccccg ctgtggactt
      181 tccgggggct gaagcggcca caacacgttt cagccgtcca ggggagagat gtaccagcca
      241 caaaactttt tgccgcaagc gtcaaaaact tttgcgtctg cccccggaca actgccccgt
      301 gccgtggcag tcgctgcccc atcggaaagg tccactgcgg ggagaaaccc cgaagaagtg
      361 gacggcagaa ggggctttcg cccctccggg ggagcaaccg gtcggcccgg cgacctgatt
      421 cagcagaagg agacgttttc atgacagtca gcagcacgat ttccgttttt tgccgcgacg
      481 gggtgttccg taccgtttac tgccacctgc acggtgagcc gacctggaac ggtcgcatcc
      541 tgcataccca ctatgccacc ggtcagcagg ccgaagccct ggttgaacac ggtgatatcc
      601 gttgcctcgg tccccgttgc gacaaacccg cagggcatac gcttcagaac ccggtggacg
      661 gtgtgacggc ttattacgga cgtgacagtg gtttccggat ggacagtgag gcgcgtgagt
      721 accgttcttt cagggaggcc atcgccacgg aaagcactga agaggtgcgc ttccattatg
      781 tgttcatcga cggctactgg aaggtgatgt accgcacgcc ggaaggctgg aagatgaaag
      841 cgctcgcgct ggcactgcgt cgctgtccgg aatgaaaaaa agcagggcga taaaccctgc
      901 ctttctccgc tggcgcttcc ccgtacggaa gtccggcatc tcattcacta actatggaga
      961 ttatgccatg agaccatcaa ttatcttcgc aaccgccgag tatgtaaagc gtctgcgaga
     1021 agagtgcctg cgggagaaca aacccctgca ccgccatacc cgcttcagac gccaggaact
     1081 ggcaccggat gagattaacc cggacgtcct ggcgatgggc ggtcatatcg ccagacgctg
     1141 cagtgagcag aagcgggtac gtatccccgc catgaaagtc agcgaatggg gccacctgct
     1201 ccgcgcgctt gaaattgaac gggtctgtca ctgattaacc cctgccgtct gcctgtgcag
     1261 gtggcaggat gtgttcaggt actttaccag gaggaaccgt gagcaagatg aaaaacatca
     1321 cgacgtctgt gttgcatggt gtctccacaa aaaacagaaa agggaaacag attctctttc
     1381 attcagggcc cgggagggct ggagataaca atgaagtctt gttttgatac tgacaaagct
     1441 tttcatatgt cttttgatga agtcactttc aacgctgaca gaattcgctc aaaactggaa
     1501 ctattatccg aagaactaat gccccgttgt ggtcttgttt atgagctata tcagcgacgt
     1561 ttgagtgctg ctattgataa ttttgtggcc acattgccga cagaacaaca tctactggtc
     1621 ctggaactgg cccgtgagga gtttgattac ctttccgcag aagagattgc tgaagaaata
     1681 cgccgtgact cagaacgcgg ctattgttgt catggctttg aacgcaattg ctgtcctctc
     1741 ggttgtgggg atttgggcga tgattgctgc gatgctgctg atttgatgga ggaaccgtga
     1801 gtaagaagaa aaacaccaca acgcccacgc cgcatgatgc cgcgttccgg tcgttcctgg
     1861 cgaatcccga cgtcgccaga gattttctgg aactgcatct tcctgcggag taccggcagt
     1921 tgtgcgacct gtccacgctg aagctggaac ccgccacctt tgtagagccg gacctgcatc
     1981 agtacgccag cgatatcctc tggagcgtga aaaccaccgg gggtgaagac ggctatgttt
     2041 atacgctcat cgagcaccag agcacagaaa atctgtacat gccttttcgc atgttacgct
     2101 acagtgtggc ggcgatgcag agacatctgg agcagcacaa aacgttgcca ctggtgattc
     2161 cggtgctgtt ctatcacggt gagcgcagcc cgtacccgta cagcatgaac tggctggact
     2221 gttttgagaa tccggcactt gcggctaaaa tatacacaaa gccgtttccg ctggttgata
     2281 tcactgtcgt tgatgacaat gaaatcatga atcatcgccg gatggccgca ctgacgctgc
     2341 tgatgaagca tatccgccat cgtgacatga tggagctgct ggacaaactc ccgaaggtca
     2401 tggtggaaat ttcagacgag caggtgcgtg ttctgattca ttacatcgtt aacgcagggg
     2461 actctgtatc accggaattt atgcgggcgc tggctgagcg tctgccgcag catgaggata
     2521 aactgatgac tatcgcagaa cgtcttgagc aaaaaggtcg ccaggaaggc aggatggaag
     2581 gacgcatgga aggagcgctt gaaaaagccc tggctattgc gtgccagctt cagaaaatgg
     2641 ggatgacgcc ggagcagatt aagcaggcta ccggactttc cgatgacgaa ctgaagaaaa
     2701 tcactcactg atgagaacca caaagcccgg caaaatgccg ggcttttttg tcagcaggag
     2761 cagagcaaac agcatgacga ttgcagaacg tcttatacag aaaggtttcg acgagggttt
     2821 taaacaaggg gctcttgaag tggcgcggga aatagcctgc cggcttcggg atataggctg
     2881 gccgccggaa cggattcagg aggtcacggg actttccggt gaagaactga aaaagctgtt
     2941 tcctgatgag cagtagcctg gcattcagcc aggctgtgaa tcacgctcac acattccctg
     3001 ccgtttcccc cagttcattt tccagtcgcc tgatgatatc tgccttacgc atcccctgca
     3061 tgtgaggcag caggtcacgg actgaaagtg cctgggtggt cctccagacg gtgacgtcca
     3121 cctgttccgc attttcccag gtgtgccatg ttctctgcgt cacgccaaat tctctggccg
     3181 ctttttcgcg tgaccagagc atgctttttc gccagattcg cagttcccag ccggtcataa
     3241 aacacctttg aaaaagtgaa aaaacttcac tttattttat catcacaatt gccaaatcag
     3301 cgcgataaat aagtgtaaaa aaatgaagaa atttcacttt tttttgcttg taaatgtcgt
     3361 gcggttttct ctgttttcac ctgtgcggga gtggtgtgcc agtgaccacc acatgccatt
     3421 gttgtgccgt cgtttttctt cagtcgcgcc gtatgcggcg ctccctgcac accttacggt
     3481 gtgtaaggcc actgcgcctg atggcgcagc tgtctctgcc tgtctgccgc cgcactgcgg
     3541 cctgttctcc gcagacggac tttccggggg tacatacatg accacatccc gtttcagtcg
     3601 tccaggggag agatgtacca gccacaaaac tttttgccgc aggcgtcaaa aacttttgcg
     3661 tctgcccccg gacaactgcc ccgtgacgtg gtgagcgtcc cccgtcggaa aggtccactg
     3721 cggggaaatc cccgaagacc cggacggcag aaggagcttc gctcctccgg gaaatgacct
     3781 ggctgacccg gcagtcgtga cgatgaggag acaatgtgat gaatcagact ttacccactg
     3841 ctgacctgaa tactgccggc acgacagatg ttattccgtc tgtggctatc gaccgcatca
     3901 tcgcgcagcg taacgaaggt attgcactgt tcatgcaggc gatggaatgc ctggcgacag
     3961 cgcgcaagat tctgctcgat gcgtcaggtg atatttttct ttacgggttt gaagactgcg
     4021 tgactgattc cgttcgtcgc atggataagc aggaagaagc gaaaaggaat atcacccgtc
     4081 ttgccgaccg gaaaatctgg gaccgcctga tgacagatac gggcatgtac accttcatga
     4141 gttcatgcca gcgtgatgag tggaacagcc agctgatgag cgacacctgt cctgaaatca
     4201 ccctggacaa tgtactggca acattccgcc atcttaatgc cagcaagatg cagacgtttg
     4261 aacaggggct gattgatgtc taccggaaat tgtcatggga ttacagaacc aataatccct
     4321 gccgtctggg taagaaaatc attattgaaa acctgctgta ccgctggagt aacgggcgtg
     4381 tgacgctgga ctgcagcgga cgggaggcac tggatgacct ggtacgtccg ttttatctgc
     4441 tggaggggcg caacgttcct gacttcagga acagcatcgg ggcgcagtat ggtgaatttc
     4501 tcgggaacgg cgacaatgtc ggtaagctgt tagaagggga atattttacg gtgcgtggct
     4561 accagaaagg gaccgtacac attgtcttta agcgttctga ccttgttgaa aaactgaatg
     4621 atattattgc acggcattat ccaggtgcat tgccgccacg agtctgatta aacagaaaag
     4681 ccctggtatt tatgccgggg cttttttttc tgtaatcatc ataatctcag aacacgatgt
     4741 gacattgtca catcaattgt tgttcatatc gatgccatct ttcctgcctg aatctgtctt
     4801 tttaggtgct gttttagagg aagaacggcg ggatggtttt ttcgattctg cttcccgaat
     4861 aatgtcacga agtgccttct ctcctacttc ataccctgca tttttgagtg tgtcacgaac
     4921 atctgccagt gtataaccct ttgtccttac caggacaata atgtcatcac gaatggcatc
     4981 aagaaaatca cgcaatgttt tccgttgagc ggtgaggtct ggcaattccg ataaagcagc
     5041 ttttgccagc tggatgtcat catctgagta aaatttttta gacgccattg gcactttctc
     5101 tccgtttaat gttgaagcct gattttatca tcaaaaactg actcctggca gggctgcgcc
     5161 ctgcctctgc cgtctgcata agactatgat gcacaaaaat aacaggctat aatggcctga
     5221 aaaacgggac aggatgtggg aatgtcggac cgcgcacgcg cactcagaca ttccccacct
     5281 ggtcagggct ttgccccgac accccggtaa ggagcagacc atgagtgaga agaaaacccg
     5341 aacgggcagt gagaacagga agagaattgt aaaattcacg gcgagattca cggaggatga
     5401 ggctgaaatc gtaagagaaa aagcggagtc aagtggacag acggtttcaa catttatacg
     5461 aagctcttca ctggataagg tggttaactg ccgtactgac gaccgtatga ttgatgagat
     5521 tatgcgtctt ggccgccttc agaagcatct gtttgtggag ggaaaacgca ctggtgataa
     5581 ggagtatgca gaggttcttg ttgccattac tgaatatgtc aatgctctgc gaagagactt
     5641 aatgggacgc tgagataact gctgatgaat gccattattc cgcacaaaag acgtgacggc
     5701 aggtcttcct ttgaagacct gattgcgtac acgtctgtgc gggatgatgt gcaggaaaat
     5761 gagctcacag aagacagcga aatcaagagt gatgtccctc accgcaaccg tttcagacgg
     5821 cttgttgatt atgcaacacg cctgcgtaac gaaaaatttg tctccctgat tgatgtgatg
     5881 aaagacggca gtcagtgggt aaatttctat ggcgtaacct gtttccataa ctgcaattca
     5941 ctggaaacag ctgctgaaga gatgcagtac aaggcggaca aagctgtttt ttcccgtaat
     6001 gatacagacc ccgttttcca ctacattctt tcctggcctg cgcatgaaag cccgcgtcct
     6061 gagcagcttt ttgactgtgt gcgtcacaca ctgaaatctc ttgaactctc aaaacatcag
     6121 tacgtggctg ccgttcatac ggacacggat aatctgcatg tgcatgttgc cgtgaatcgt
     6181 gtacatcctg aaactggtta tattaactgg ctttcctgga gccaggaaaa actcagccgt
     6241 gcatgtcgtg aacttgaact taagcacgga ttcgcgcctg ataatggctg ctttgtgcat
     6301 gcacccggca accgtattgt gcgcaaaaca gcgctggtac gtgagcgccg taatgcctgg
     6361 cgacgcggga aaaagcagac gttcagggaa tatattgcgc agatgtcgat tgccgggctg
     6421 cgtgaagagc ccgcacaaga ctggttgtct ttgcataaac ggctggccag tgatggtctg
     6481 tatatcacga tgcaggaagg ggaactggtg gtgaaggatg gctgggacag agcgcgggaa
     6541 ggtgttgcac tcagttcgtt cggaccgtca tggacagctg aaaaactggg gcggaaactg
     6601 ggggaatatc agcctgtacc gacagatatt ttcagtcagg tggggacacc cggtcgttat
     6661 gatccggaag ccataaacgt tgatatccgg ccggaaaaag tggcagaaac ggagagtctg
     6721 aaacagtacg cctgccgtta ttttgccgaa cgcttaccgg aaatggcgag aaatggtgag
     6781 ctggaaagtt gcctggatgt gcacagaact ctggcaaagg ctggattatg gatggggatc
     6841 cagcacgggc atctggtact gcatgatgga tttgataaac aacagacacc agtacgtgca
     6901 gacagtgtgt ggccgttaat gacgctggat tatatgcagg atctggatgg tggatggcag
     6961 cctgtaccga aggatatttt tactcaggtt atacccggag aacgattccg gggacgtaat
     7021 cttggtactc aggctgtcag tgattacgaa tggtatcgta tgcggatggg gacaggtcca
     7081 cagggagcaa taaagcgcga actgttttct gataaggaaa gtctgtgggg gtacacgact
     7141 gttcagtgtg agtcgttgat tgaagatatg atcgcgggcg ggaatttcag ctggcaggcg
     7201 tgccatgaga tgtttgcgcg taaagggctt atgttgcaga aacagcatca tggtcttgtg
     7261 attgtggatg cgtttaatca tgagctcaca ccagtgaagg ccagcagtat ccacccggat
     7321 ttgacgttat ccagggctga gctgcaggca gggccatttg agattgcagg tgcagatata
     7381 tttgaacggg tgaagcctga atgtcgctac aatccggaac ttgctgccag cgatgaagtg
     7441 gaacccggct tccggcgcga tcctgtactg cgtcgtgaac gccgtgaagc ccgtgcagct
     7501 gcgcgggaag atttgcgcgc gcgttatctt gcatggaagg agcactggcg taaacccgac
     7561 ttacgttacg gagaacggtt acgggaaatt catgctgcct gccgtcggcg caaggcgtat
     7621 atccgtgtgc agttccggga tccacaattg cgtaagctgc attaccatat tgcggaagtg
     7681 cagcgtatgc aggcgctgat caggctgaag gagagtgtga aggaagagcg gttgtcactg
     7741 attgcggagg gtaagtggta tcctttgtcc taccggcagt gggtggagca acaggcggta
     7801 cagggagaca gagctgcatt atcacagtta cgtggctggg attatcgtga tcgtcgcaaa
     7861 gacaagcgac gaacaacgaa tgccgaccgc tgcgtgattc tctgtgaacc gggaggaact
     7921 ccactttatg aagacactgg cgttctcgaa gcccgtctgc agaaagatgg cagtgtgcgt
     7981 tttcgtgatc ggaggaatgg cgagttagtc tgtgtggatt atggggaccg tgtggtgttc
     8041 taccatcatc aggacagaaa tgaactggtg gataagctga atctgattgc tcctgtattg
     8101 tttgatcgtg agccaggaat gggttttgaa ccggaaggct catatcaaca gtttaatgat
     8161 gtatttgcgg aaatggtggc ctggcataat gcagctggaa taaccggaaa tggacacttt
     8221 gtaatcagcc gcccggatgt ggatttacat cgtcagcgta gtgaacagta ctaccacgaa
     8281 tatatcaggc aacagaaaag catatccggg gggcatggcg catcttatgc tccagttcag
     8341 gataatgagt ggacgccacc atcacctgga atgtagggat gaggagtgga tattaaaaat
     8401 cttaacttgt attagttaag attttctttg ctctgacaga gctatggtac tgagatctat
     8461 atggattgag gagcaatata tgggggtaaa gaaaatcgtt atgctggcta ttttagtcag
     8521 ctttgtggcg ggatgtgcgc cattacatcc ttcaaattgc cataaaacta cagccctggg
     8581 tagttgcagt tcaggacgtt gggatgatca ggatgaatgg ggggagcagg cacggggaat
     8641 cagggatgca attaatgcta aacttgatga tccgcaaaaa tggaaaagga aaaaatgcag
     8701 gttgcatatg gaattctccc gggatggcac ggcgttaaaa atatcaacca gtaatggcga
     8761 taaagcctat tgcgaagcga taaagtcagc agcccataaa gctaaattcc cggccttcaa
     8821 caatccggaa gtttatagag attttcaaaa atccggcttt gacatgcgcg gttagctcca
     8881 cgacatatct gtagggctaa cagaagactg tcaggttggc tgattactac actggaacct
     8941 gaggtacttt gcctcaggtt atgttgacat actcaaaaat agattcttac attatcccgg
     9001 taacggcgtc tttcatcggc ctgccagtta ccatcaatgg tattggtacc ctcgttcttt
     9061 ctgtctgtga tgttaataag ggcaaaatta atcagcatgt ctggcaggag tgacgggcac
     9121 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     9181 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     9241 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     9301 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     9361 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc
     9421 ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg
     9481 ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa
     9541 gtgccgcctt gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga
     9601 gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta
     9661 cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga
     9721 gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc
     9781 tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc
     9841 gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc
     9901 catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa
     9961 catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca
    10021 gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc
    10081 cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca
    10141 acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg
    10201 gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc
    10261 tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc
    10321 cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct
    10381 cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg
    10441 tttcaatccc ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct
    10501 ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg
    10561 cggcatgaac atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg
    10621 cggtcggggg cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca
    10681 tctggcgctg cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga
    10741 tctgctgttc cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc
    10801 accatgaagt tcggcatgca gggatcacca tggcaaacaa ccatatcgtc gctatcaatc
    10861 gcggaaaaag gcgtagtgcg cgaagcgaac gaagcttttt gccgtcgatt gcatagcttt
    10921 gttaaccctt tttccaaatt tgatagcaat agttaatgtt tgaagtaaag tgttgctcaa
    10981 aaacaacttc gaaggtattt ggaatactcg ggaagaaaac atccccctct ggctcgatgt
    11041 cgatcgtcga taagtggagc gtagaggcca tgggtaatgt ttctcggtaa atttctccgc
    11101 caccagacac tataacgtga ccggtgaatt cagctagcct gtccatggcc tcttcgattg
    11161 actgaaatac aactacattg tcatcatttg atgtccaacc tgagcgggta acgaccgcgt
    11221 atttcctatt ggggagtgcg cccatagatt caaacgtctt gcgacccacc agaagccact
    11281 gattgtaggt caatgcttta aaaagtagct gctccccttt cgcggaccag ggtatgtctg
    11341 gaccgcaacc aatcacgccg tttttcgctt tcgcagctat caatgatact ttcaaggttc
    11401 tcatcctggg ttaacggtgc gctcttggtc gagccgcacc ggtagctctc gttcgacacg
    11461 agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt cgggattgac
    11521 ggcattgcgg gacaccacat caacggcgcg tccgaacatt cgcgacagcc tgcgctcaaa
    11581 cggacattga tcaaccgata ggctgtgaac agcgccaagt tgctgcccca ttgacggcca
    11641 cgctttgagc aaatccgctc cagacagatc agccgccggt actcccggaa ttgccgttat
    11701 caccaagcat gcaccctcct gttcctcctg ccagttgatg acctcggggc aagccacacc
    11761 tcgacctttg agccaaatga ggcggtcacg ctctccagcg agctcaccgc ggcgggaagc
    11821 aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat caccagattc
    11881 tccgcctctg acaggcaacc agtcagaatg cgattcacca aaaaaaatat tagttcgatt
    11941 caatggaggt tccttcagtt ttctgatgaa gcgcgaatat agagaaatat cccgaatgtg
    12001 cagttaacga attcttgcgg tttcttttag cgccgccaat accgccagcc cgtcgcgcaa
    12061 ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct gcggcggcgg caagctctgc
    12121 agcgagtgtc gcggccccga catcccccgg accacggcct gtgagcgcgc gcagaaagga
    12181 tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc agccgcaatt catcgaaccg
    12241 cgccagcacc gagagcgagg tttcgggagc agcccccaga aaaaacccca tgccgggatc
    12301 aaggacaagg cggttgcgtt tgataccggc acccgtcagc gccgcgatgc gcgcgtcaaa
    12361 gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc tcgcgccgat ctgcctgccc
    12421 gtcttgcacc gaatgcataa cgacgagttt ggcagatgat ttcgccaatt gcggatagaa
    12481 cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc acaccacgcg acaaggcata
    12541 ggcttgcgtc gcgggttgat aactgtcgag cgagacggga atgccatctg ccttgagcgc
    12601 gtccagcacc ggcgcgatac gcgcgatttc tgtgtcggac gaaacaggcg cggcgtcggg
    12661 attgctggat gccggaccga ggtcgatcac atctgccccc tcggccatca gcttacgcgc
    12721 ctgcgcaatg gctgcgtctg gcgccagata ccggcctcca tcggagaaac tgtccgaggt
    12781 tatgttgacg atgccgaaaa tgatgagcga tttattcatg ggggcttcta taataataat
    12841 aatcgagcat gagtctcata cggatgctcg ggtcgaaagg gaatccccag gcgagtaacc
    12901 tgtttgcggt gatccattag ctgcaggagc agaatagcat acatctggaa gcaaagccag
    12961 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
    13021 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
    13081 gaatataaaa gataaaaatg tcttgtttac aatagagtgg gatctgggaa ttcataccaa
    13141 tatccataga agtgaatcta ttacgaacgt ggggaaccct ggcaccattt tttgtgctgc
    13201 ccatatttgt tttcatgtat aattcataat caaagcgttc atcctgattt agcttaactg
    13261 attaaaatcc gtagattatg attttatcag ttttgtgact ttatttatta tggggagctt
    13321 acccagtatt tcgaaattat cgggagaaca cttgtaccat ctgtttgagc ctgcaagtat
    13381 ttctctgtcc catcgtctga ccctctcccc gtttctgagt atgcatatca tatagttttc
    13441 gggcaccatc agtgtccggg ctgccagaag actattttta aacgtcaggg aagtgatatt
    13501 gatatcttct ccggaatttt tacgataaat aacagaccat gagtttgttt ccgggctact
    13561 aatacacatt tattgtacct ctggtgatgg attgaaatcc tgaatcctcc gcttactatg
    13621 gtgagcggag gagattaata atcgcgtaaa ggttgttatt aaaatcagcc atacaggtgt
    13681 tatcagaatc tttttttacg actctcttgt tttccacctg atggtgtctg agcgacattt
    13741 tttttatggg gcggggatgc agttagatcc ggtacaggta acagcatctc cagggggagc
    13801 agagtattca gaatcgcctt cactgaaggt atatcttcag gaccggagtg ttcctcttcc
    13861 ggacgattca gtgtgatata acatacccgg cgttcttccc tggttgtgtt attgatagtg
    13921 tgcacagcct ttttccacag ttcgtttgcc cgggattcga tatctgtatc atattgcgga
    13981 agcagatcca gtgatgtctg cgctgttgcc aactggcgct ggaatgtatc aatgatacgg
    14041 taaggctctg tacggttttt acgacgttta cgggatggtt ttgctgtcag tacaccgatg
    14101 atgctgctga catgctccgg tgtggggagc ctgcgctgca ctgtcctggg gacgagacgg
    14161 cggagacgct ccagccgggg ggcttcaacg gaaatatagc ggttgatgac caccggatca
    14221 ctgtcgcaac ttttctcgct gtccggaata tacacagagg ctgacggcac cacatccccc
    14281 tggaaaacgg taaagaactc accagcattc agtctgatca aatcctgcac gtcgattttg
    14341 ctttccatct ggatggtgat ctggttaccg tccacccagg actcacccat cacaccatcg
    14401 tggcgctgca ggctgcccat tctggcccgt gcctcttcac cggcggcatt tttcaccgtt
    14461 cttgccgttt tgtcgtcact gacaatgcgt ccggcgaatt tggtgccggc gttctgcatc
    14521 agtgtggcgg tgttggtggc ggaggtctgc ccttcaattc gctcctgatc ctgcgccgtc
    14581 attatcaggg aaaactccag tgaacgtacc tgggtggcct ccacagcaat gcggtctgtg
    14641 tagtaggcac cgacttcgtc aaggatacag atatagggga agctgccttt gtattttttg
    14701 acttccaggg tctgtgcatc tgtgccttcc agccggtagc ccaggtcacg ggcgaggagc
    14761 atgctctgct gggtgacgaa catacgaccg agtgccgacg tggtatgagc cgatgtgtcc
    14821 agtgcaggga tcatcacaat cagaatgcgg tcgctgtgaa tactgtcgcg aatatcgatg
    14881 tcgccggcat ctgccgcaaa gacgtccccg aaggtttcgg caaaggttgt gaacgtttcc
    14941 gagaactgac cggagagata agcatgctgt ttacggggct cctcagtcca ggctgacggc
    15001 gttctcacca gtgacatatc gaatcccggg acatcctgca ggtagtttcg cagtggtgcg
    15061 atagcttctt ccggccactg gtcatcaagg ccacggcaat agagttttgc catcccttcc
    15121 agcgtcatat gttcccgtaa catctgcagg gacatggttt taccttcccg gacgcaccag
    15181 aattttgtgc caaagaccag ggccttgttc atggctatcg cccgtgactg ccattcaccg
    15241 ccctgaacgt tctggggcag catggactgc atggtttcgg cggtgaaggc ttcggtgctg
    15301 aatgcgaacg ggttccaggt gtttgactgt gggcgggatt tttcgccgct ctgaatgatt
    15361 tcactgcggg attttccgcc gttcatgaag ttgatgactt cgatatcatc ctccctgcca
    15421 aaacgtcggg agagatacca gattgtcctc gtggtatcgt tctgtgcctt tccgtcaaca
    15481 aaggtaaagc ctcttcccca gcagagcggg ttgagcagcc aggcaaaggt ggtttctgtt
    15541 ttaccgccac cggtggtggc aaaaaacatg acatgccggg tgaggtcatc catgctcagc
    15601 cagagctcac gaccgatatc gttgatacgg cggtaaccga cataaaaaat cccccgggcg
    15661 gggaatgttt catccgcttc atactgaaac agggtgggcc agaagctgaa gaggcttctc
    15721 ctgacctttc tgtcctggga ggggtcatca aggctgagtg tcatgggcat acgcaggggg
    15781 catcgccagc gttgcagcat gaatatcacg gaggtgacca gggtgaacag caggcagaca
    15841 ggaagggcca gtggccatat cagcgccgta atgatactgc cggccagaca cagaccagtg
    15901 atgttcaggt tatgcagtgc attccacaac gggtttcccc aggccgtgcg gtgtatgagg
    15961 tcaggattaa cgtggtgctg gctcattgtg atactcctcg ctatctgttg ctttcaggaa
    16021 gaggcgaacg gaagtggcgt ctttcaggat gccggacggc aggtgccagc cggtatctga
    16081 taacacccag ggggttccgc caaaaccgaa ctggcggaaa gcctggttat tgacatcgat
    16141 ggcagcacgt gcggcggtca gacactgtcc atcctgtgcg gccggtgcat cgccggtggg
    16201 ggccatacca ctgtcagcgg aatacagctg atgccatccg gtcgcccgtt tttgtgagtc
    16261 cggttcacac agcagcggag cagtccgctt tgtactggac tccccgccaa tcacggagac
    16321 cggataaatc accacgctga attcccgggc cagttgctta agcgcgggtt ccagcagacg
    16381 gcagttgatg catgcggggt caaggaaggc gtacagcgtg cgttcatgac cagtggacag
    16441 gttaatggtg aacatccccc ggtctgcgtt gcgtttaagt actgtggcca gattacggcg
    16501 ggcctcagcg gcctgaacgt gttgttcttc cgtcactgcc gtcggggcgg gggcagaaga
    16561 aggttcctgc tgtgtgggag ccgggcgggc cgggatgctg ttaacatgat gttccgtatg
    16621 tgggcgtggt gtctctgtgt cggttactgg ttcagctctc cattcctgag atgtagggta
    16681 agccagcgtg gaagggggct ctgccttcat ccagtggtaa cctgcaactg tcgccggcag
    16741 agcaattccg gcgataaagc cacacatcag tgtccgccag aagccatttt tccgggaggg
    16801 atgttttcct tttccggcat attcggcatc caggcaggca tcaatcgctg agagcattct
    16861 gccgatgtga tgtccgggac gttctgcatc aacggtatca acaagcaccg tttcggttat
    16921 ttcgcgaaaa ggggaactga aggacaggtg catcagcctg ttattacggt taatcagtgc
    16981 gcccagttct gtgactggca ggatggcaat cacctcccga ttaccgtcag ggtacagggc
    17041 gatcagggac aggtgtttat tattcagtgt tgcagtgtag tggatggtca ttgttctttt
    17101 tccttgtcgg cgggatgctg tttttcggtg gtttcggtgg tttcggtggt ttcggtggtt
    17161 tcggtggttt cggtggtttc ggtggtttcg gtggtttcac cagtgtcagt gtcttcgtcg
    17221 ttatcatggc ggcggggtat cacgtcatcc gtgagtgacc agcgggtcct gatctgacgt
    17281 ttacgatcgc gatctggctg aggttcgtcc catatcaggc ccagccccag catgtcccgt
    17341 cgaagccctt caatggcctc ttccacacag ggttccgggc aggggaggcc gagacggctg
    17401 gcttcatttt ctgcccgggc aacggccacc acgccggccc cttcgatgaa gccttttgtg
    17461 gtgtttgccc ggtgaagggc atagaagagc gtccggtcaa cgcccttgag ccacagccag
    17521 ttcggaggcg tcaggcgcaa atcatgtgcg taaagccaga ccagaccgct gcgaacgtaa
    17581 cggtgctgcc tgagccactg ttgtgcgcct tctgttttga tgacccggat aaaggcgttt
    17641 ctggccagtg aatacaccgg cgtgctgaaa cgcccctggt cccgtttact tttgagcctg
    17701 caggagagat tgaggttgtt catcagggct accgctgctt tccggtcacc gaggaagaac
    17761 tgcaggccaa agatggcaaa gagcgctttt tcatgtgggg ccatgtcttt ccagctggtc
    17821 agtggttgcc ccagttgcgc catgaagcac tggcgtgtgg acgcaacatc gagctgcatg
    17881 ttgcggataa ggttgttctg ctccacaaag acttcggggg ttagtgcgac acggcgctcg
    17941 ggacgttttt gtccgtggaa gagtctgttg ttgtctccat cagccagaac gggggcgatc
    18001 gccggtgaca gagctgaaaa aatatggggc agtgtgtgga tattcagtgg cctgcgggta
    18061 aaacggggct gggccggatg gtgaaaccac atccacagaa agccgaatgt catgggaagc
    18121 agaatcaacc agaggatccc cactgtccgg gacataacat ccagccactg gccgaaagtg
    18181 atggttgccg cgttgttagc agtgatggcc agcaggttgt aacggggggc ggcaaagttg
    18241 tgaaagggcc cccagtcaat catgccccac agtgtgtgca ggatcaggca ggaggcgaag
    18301 acgatttcag gtaaaaagcg ccagatgata atcgcaaaga gcaggcatat ggcaccgacg
    18361 atataccata tagccattgg atcttcggcg gcagacgtct gttggtaaga cacggggatt
    18421 cctccatcga taaatagata aatttcggga tgaggctgag tggaattaat gcgaacaatc
    18481 gccggtaatg actgggcagg gctgaattca gcagggcatt ttcacaggaa cagcagaagg
    18541 tggtgagaga caggcacaaa aaagccgact ggtgaggccg gcttttttgc gatcccaggg
    18601 agagtgggtg gctctggccc ggaggaagtc tcttttcaac taacgttacc agaatatcgt
    18661 tatattcacc tgatcgcaat ctgttcgtta cagtctattg ttccgggagt agcgatatcg
    18721 cctgaaacaa gtataatcag caagttagcc ttgtctggag acttaccttc gcatcaatcc
    18781 atcgggacta tggatctgtg ctattcaaag acctggccgg acaaacaggt ggtgctgcgt
    18841 ctggtgctct ggccggagga agtctctttt cagctaacaa tgaaagagct gttgtgacag
    18901 gcaatgtaag agtaaacaac agtgatttcc tccattgatg gagaa
//
LOCUS       DAZFRE010000093        18684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFRE010000093 DAZFRE010000000
VERSION     DAZFRE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.093"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(19..895)
                     /locus_tag="ACHKUI_003815"
                     /pseudo
     CDS             complement(19..895)
                     /locus_tag="ACHKUI_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799662.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(881..960)
                     /locus_tag="ACHKUI_003816"
                     /pseudo
     CDS             complement(881..960)
                     /locus_tag="ACHKUI_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067459.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            1291..1422
                     /locus_tag="ACHKUI_003817"
                     /pseudo
     CDS             1291..1422
                     /locus_tag="ACHKUI_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1525..1794)
                     /gene="stxB2"
                     /locus_tag="ACHKUI_003818"
     CDS             complement(1525..1794)
                     /gene="stxB2"
                     /locus_tag="ACHKUI_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit B"
                     /protein_id="HFO6599926.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCASGSGFAEVQFNND"
     gene            complement(1806..2765)
                     /locus_tag="ACHKUI_003819"
     CDS             complement(1806..2765)
                     /locus_tag="ACHKUI_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2k subunit A"
                     /protein_id="HFO6599927.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSQEFTIDFSTQQSYVSSLNSIRT
                     AISTPLEHISQGATSVSVINHTPPGSYISVGIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISLPGVTTISMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTREASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPEDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE"
     gene            complement(2857..2933)
                     /locus_tag="ACHKUI_003820"
     tRNA            complement(2857..2933)
                     /locus_tag="ACHKUI_003820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2897..2899),aa:Arg,seq:tct)
     gene            complement(2947..3023)
                     /locus_tag="ACHKUI_003821"
     tRNA            complement(2947..3023)
                     /locus_tag="ACHKUI_003821"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2987..2989),aa:Arg,seq:tcg)
     gene            complement(3033..3108)
                     /locus_tag="ACHKUI_003822"
     tRNA            complement(3033..3108)
                     /locus_tag="ACHKUI_003822"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3073..3075),aa:Ile,seq:cat)
     gene            complement(3135..3848)
                     /locus_tag="ACHKUI_003823"
     CDS             complement(3135..3848)
                     /locus_tag="ACHKUI_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599928.1"
                     /translation="MDVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISHSIVSQYLESQHKLTRGPLTDIPLYLLLDPNNPALAAALITSQRFSGE
                     TTDMFLMMACLSLFESDERMSLFLSGCVSSISVKVRAIIQTDISSSWTLGAIALQLHM
                     SESLLKKKLKDEGLRFSNLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSIQGLDF"
     gene            complement(3985..4182)
                     /locus_tag="ACHKUI_003824"
     CDS             complement(3985..4182)
                     /locus_tag="ACHKUI_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6599929.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(4398..5626)
                     /locus_tag="ACHKUI_003825"
                     /pseudo
     CDS             complement(4398..5626)
                     /locus_tag="ACHKUI_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(5721..6101)
                     /locus_tag="ACHKUI_003826"
     CDS             complement(5721..6101)
                     /locus_tag="ACHKUI_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6599930.1"
                     /translation="MRDIQMILERWGAWAANEGGSVYYSPIAAGFKSILPFSGKVRPI
                     CSDDDGLIISSAMNALKKKDPYLCTLLELHYIHSISARNIAKRQGISHTQILKRLQKA
                     EGFIDGCLSIMNVKLDVDGALHHG"
     gene            complement(6101..6358)
                     /locus_tag="ACHKUI_003827"
     CDS             complement(6101..6358)
                     /locus_tag="ACHKUI_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599931.1"
                     /translation="MNNQTMTFTPEQLRKQAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            complement(6355..7746)
                     /locus_tag="ACHKUI_003828"
     CDS             complement(6355..7746)
                     /locus_tag="ACHKUI_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6599932.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNKALQKLRDAHTCEAAEDALKDAQNMMASLSTEKGIIQPVHIDDVLPEV
                     VERIECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVTHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTVYRRFYNGHFLPVDQESAQVLSTPMQQPQPRRYS
                     NKRTDSSKMERFF"
     gene            complement(7743..8621)
                     /locus_tag="ACHKUI_003829"
     CDS             complement(7743..8621)
                     /locus_tag="ACHKUI_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6599933.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGKYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIEHYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(8731..9456)
                     /locus_tag="ACHKUI_003830"
                     /pseudo
     CDS             complement(8731..9456)
                     /locus_tag="ACHKUI_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(9453..9677)
                     /locus_tag="ACHKUI_003831"
     CDS             complement(9453..9677)
                     /locus_tag="ACHKUI_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599934.1"
                     /translation="MIRRIVNSLYHRYNRCPCVGQWFTTSNGHVLRVCLVSTESQKVV
                     CELLGRNYTISYPLVVFQSGKMFKRLGGVL"
     gene            complement(9674..9907)
                     /locus_tag="ACHKUI_003832"
     CDS             complement(9674..9907)
                     /locus_tag="ACHKUI_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599935.1"
                     /translation="MCNNHYSDTSVTVIKTLMDALIDISVIADMAHKHATTETEYAGA
                     FVPHSLAVMQISADQALETASKMLMADVQEVRP"
     gene            complement(9900..10562)
                     /locus_tag="ACHKUI_003833"
     CDS             complement(9900..10562)
                     /locus_tag="ACHKUI_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO6599936.1"
                     /translation="MIVLQNIDSAISGRYTVPAPYKAGAGGRSPELHTAIYDALASFL
                     SSALTHARIMVGWAGEPKGSPVSVCAGTANPVQFTTHEFRSSGGGSYPLHTEAAIMAT
                     VPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTYH
                     VYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHKAETSLSHTAFRRNQWEAS
                     HV"
     gene            complement(10559..11383)
                     /locus_tag="ACHKUI_003834"
     CDS             complement(10559..11383)
                     /locus_tag="ACHKUI_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO6599937.1"
                     /translation="MKNNIKVFDFKSSTGELLSSVRSVVIDSTPWFFAVDICNALGLT
                     NTAISLQSIDDEDKTEYKDYLGSGRKPLLVNESGLYALIIKSRKKQARRFKRWITSEV
                     IPSIRKTGNYCLTTMTSLPDFSDPAAAARAWADEYEAKNRAISYVHRQAQYIEHLENL
                     FQPGMTPVQFCKQLNGVNVQRITAFLEAHNWLYDERPESRSPAWRVKAYARDLYLTER
                     HHYIDSGYEEGFYSYTPVLLQKGAVWIYRQYLRGALPMKRNWNGEFTHDKELAGAA"
     gene            complement(11436..12143)
                     /locus_tag="ACHKUI_003835"
     CDS             complement(11436..12143)
                     /locus_tag="ACHKUI_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6599938.1"
                     /translation="MNAAIFNDKVSMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQYEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQK"
     gene            12315..12560
                     /locus_tag="ACHKUI_003836"
     CDS             12315..12560
                     /locus_tag="ACHKUI_003836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599939.1"
                     /translation="MSNTQHLKAADPVLRLNALETVVRAMLSSMTPEQINKARLIATR
                     GAQLAALDPLLTPANREDVEHIQGMVDYLFAHIPDQI"
     gene            complement(12642..12887)
                     /locus_tag="ACHKUI_003837"
     CDS             complement(12642..12887)
                     /locus_tag="ACHKUI_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6599940.1"
                     /translation="MKHKAIEKAIEILGSQQALAKRCGKAQSTVCDWLNCKNKISPEF
                     VPLLVAAVDGKIQAHEFRPDLPELFPHPSLAHTEDEK"
     gene            13007..13699
                     /locus_tag="ACHKUI_003838"
     CDS             13007..13699
                     /locus_tag="ACHKUI_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6599941.1"
                     /translation="MTFSERLDLAMRNAKFTQGRLAKEVGMAQSSVNQLLNKANGSRK
                     TVEIAKVLGVNPEWLASGVGPMEIKTSADSHQIRNISEDWVTDSYVVDVLDIRYSCGP
                     GSYNSDFPDIVRSIAIEPGYASRVFGGRPASAIKAINAHGDSMKGTIDPEDLVFVDVS
                     VRTFNGDGIYAFTYSGTSHIKRLQKIKDTLTVISDNPAYKDWAIEPEDLEQLHIDGKV
                     IVSWPMTLHRFA"
     gene            13845..14255
                     /locus_tag="ACHKUI_003839"
     CDS             13845..14255
                     /locus_tag="ACHKUI_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6599942.1"
                     /translation="MSTYMGLPTNQHDALENIQLLVGTSALLYSSNNPELIELASSIL
                     DVTQKYSLTAARSYKQNFAINSRNRLRTQREACGLTTAELARLLALDEEIIIQWESGE
                     YEPTISMLIPLANVLGCDPMWLLTGKPTAGDTCA"
     gene            14411..14617
                     /locus_tag="ACHKUI_003840"
     CDS             14411..14617
                     /locus_tag="ACHKUI_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599943.1"
                     /translation="MTETTSQQDDYEGICLEPDSFAVNVYHLLHATQVLHMSSNHETK
                     TLGSEILNFACEYAKSAAEKELAQ"
     gene            14814..15002
                     /locus_tag="ACHKUI_003841"
     CDS             14814..15002
                     /locus_tag="ACHKUI_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599944.1"
                     /translation="MEEKRQNITHKKVRVLLTIENGEVIYSKHLLDNEFVGCMDTFLW
                     MAKRAGYTIIPPAKEQTL"
     gene            14999..15580
                     /locus_tag="ACHKUI_003842"
     CDS             14999..15580
                     /locus_tag="ACHKUI_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599945.1"
                     /translation="MNHSEFRPEVTPHGIKIGNTTIDYVEAVQRLNDGEYDYPNSRGL
                     SILQCLAEAYESGILENCSIHMKVAQWRWLYVTVFINEEEYKNGTIDIPNDNGTTDRA
                     VIYKGKHGCMSIYPGPLRIALQNHVEWGFIEKYGEAEGMGRVLFLYQKMLIADPDNGF
                     IVSAMGREGLELLLDEMINDLNTHSMPEAPVTH"
     gene            15757..15945
                     /locus_tag="ACHKUI_003843"
     CDS             15757..15945
                     /locus_tag="ACHKUI_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599946.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKR"
     gene            15942..16769
                     /locus_tag="ACHKUI_003844"
     CDS             15942..16769
                     /locus_tag="ACHKUI_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6599947.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMNLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGASTPFIQVK"
     gene            16810..17181
                     /locus_tag="ACHKUI_003845"
     CDS             16810..17181
                     /locus_tag="ACHKUI_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO6599948.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMTKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGEPW
                     LAGMLISAEIISIEPATNIRL"
     gene            17213..17455
                     /locus_tag="ACHKUI_003846"
     CDS             17213..17455
                     /locus_tag="ACHKUI_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6599949.1"
                     /translation="MFALINQGQLYTDSAGYPVKIIRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERLDHQEYRQILAETEQETHLKKLRAMKRK"
     gene            17459..17605
                     /locus_tag="ACHKUI_003847"
     CDS             17459..17605
                     /locus_tag="ACHKUI_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599950.1"
                     /translation="MNKAFELWVRQRYGNRYDLTRDVDGFYCCEIVKRMFEVWCHCRG
                     LSVV"
     gene            17614..17850
                     /locus_tag="ACHKUI_003848"
     CDS             17614..17850
                     /locus_tag="ACHKUI_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6599951.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRARKECWMVGREYI
                     HVSPDGNPKPSSECMYNRKAIDAWVASMKNKQPG"
     gene            17906..>18684
                     /locus_tag="ACHKUI_003849"
     CDS             17906..>18684
                     /locus_tag="ACHKUI_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599952.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVNKKEITVKELEKKWLDLKRMEISAN
                     AFNRYESVARTMIPKIGGSRLVSTVTKEELLYIRKDLLTGYQNSTKDKAPVRGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFEGIKPLKKARAEPDPLTRDEFIRLIDACRHQQ
                     IKNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKL"
BASE COUNT         5126 a         4518 c         4025 g         5015 t
ORIGIN      
        1 aaataacaga aagcgtgttt aaagattatt ttctattaca gttacagttt gattaaatct
       61 ctctttttcc agttccacac caattgcccg acgccccagc gtcattgctg ctttaattgt
      121 ggagcctgac cccataaaaa agtcgacaac caagtctccc ggacgactgc tggcggtaat
      181 tatctgacgt aacatatccg ctggtttttc acagggatgt ttgcccggat aatactgcac
      241 tggcttgtac gtccagacat ccgtataagg aacagcagcc gatacggaaa aataacgccg
      301 taaagatttg tactctgcca gcaggctggc atattgccgg ttcagttcac tgtatgcgct
      361 gaccagctgg tgatgtggtt tttccagttc tccgcgctga tgcttttctg ctgctacacg
      421 tgcaaataac aactgcagtt ttctgtaatc accctcgttc ggcaactgcc actgactggt
      481 accaaaccag tgtgacgcca tgtttttctt tccggtggct tccgctatct gtttcgatgt
      541 tacccccagt gaatcacgcg catcacgaaa gtaagaaatc agcggggcca tgacgtgctg
      601 tttaagctcg cgcccctttg ccgcatagcc atcattttag ggccaatatg gtccctgata
      661 atgttcaaca agcagaatga acgctgtagc cgggaaatac accgtagact ctccttgctg
      721 gcccgttcca gcgcccggac ggtttcgccc agatgatatg gttaagcacg ttgaaacgct
      781 cacgcattat tatctcgata tcagatgcct agcgatctcc acagaacagg taaaagcttc
      841 cggcaggttt cagcacccgc cataaccggg ccagacagtg gtccagccac tgcagaaacc
      901 tgcactacct gctgatgtgc cgtaagcaac gtcagttgtg attttttcca tcaggtacat
      961 atctccgaac tccggaagct gacagcaaca aaaaaaagcc acccgttaat tactgatggc
     1021 cctgatgcat aaacgtcatc attcctgacc gttatgattg acaatggtta tcacttttat
     1081 ataaaaagac tcccgatatg tgtaacatat catttatcca cggggaatat ccccacgcca
     1141 gcgtcagact agtcttcccg ttctcttcta tgctggcttt tttttattac gatgtcggta
     1201 catttgtctc cagcaccagg ctgtcaatct caacgccata cgcggcattt tttggcgtaa
     1261 aaatcccctc cggtgagggg atttgattat gtgaacattt acactgctta cctgcatggt
     1321 tccgggtgcc tcccggtgaa ttcagtatca gcacctgaat ccgcaattat cccatatatc
     1381 tacttgctga ttgcccctcc gcacaggggg attcaccata cgtaaatttt ttaacaaacg
     1441 ctcagcctgt caggcaacag tcaactgact gaattgtgat acagattaca cttgttaccc
     1501 acataccacg aatcaggtta tacctcagtc attattaaac tgcacttcag caaatccgga
     1561 gcctgatgca caggtactgg atttgattgt gacagtcatt ccagtcaact gagcactttg
     1621 cagtaacggt tgcagattcc agcgactggt ccagtattct ttcccgtcaa ccttcactgt
     1681 aaatgtgtca tcctcattat acttggaaaa ctcaatttta cctttagcac aatccgccgc
     1741 cattgcatta acagaaacca atgcaaataa aaccgccata aacatcttct tcatacttaa
     1801 ctcctttatt cacccgttgt atataaagac tgtgactttc tgttcagaaa cgctgctgct
     1861 gtattacttt cccataatgt attgtttatt tttataacgg gcctgtcgcc agttatctga
     1921 cattctggtt gactctcttc attcacagcg cgaacagaac gcgcgccctg atgatggcaa
     1981 ttcagtataa cggccacagt accaagtatc gctgatatgt tattaaagga tattctcccc
     2041 actctgacac catcctctcc ccgatactcc ggaagcacat tgctgattcg cccccagttc
     2101 agagtgaggt ccacgtcttc cggcgtcatc gtataaacag gagctgtttc agacagtgcc
     2161 tgacgaaatt ctctctgtat ctgcctgaac cgtaaggctt cggctgtgac ggttacaaaa
     2221 cgcaggacag ccctagaagc ctctctggtc attgtattac cactgaactc cattaacgcc
     2281 agatatgatg aaaccagtga gtgacgactg atttgcattc cggaacgttc cagcgctgcg
     2341 acacgttgca gagtggtata actgctgtcc gttgtcatgg aaatagttgt cacaccgggc
     2401 aatgatatat gtgcaaaatc tgaaaatctg tagaaagtat ttgttgccgt attaacgaac
     2461 ccggccacat ataaattatt ttgctcaata atcagacgaa gatggtcaaa acgcgcctga
     2521 taaacatcaa gccctcgtat acctacggaa atataacttc ctggtggtgt atgattaata
     2581 acggataccg atgtagctcc ctgagatata tgttcaagag gggtcgatat cgctgtccgt
     2641 atactattta acgaagatac ataactttgt tgagtcgaaa agtctatcgt aaactcctgg
     2701 gaataggata ccgaagaaaa acccagtaac aggcacagta cccatttaaa taatatacac
     2761 ttcatataca ggtgttcctt ttggctgaag taatcagcac cagacccggc gcagatataa
     2821 aaaaggcccg caaaagcgag cccgagaaaa taagtgtggc gcgttgtact ggattcgaac
     2881 cagtgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgctgaatac
     2941 cgataatgga ccgccatcgg gtgctcgaac cccgcgcagc cagcttcgaa ggctggcgct
     3001 ctgtcccgat gagctaatgg cggtatgtga tatggtggcc cttgctggat ttgaaccagc
     3061 gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccga gccgaaaata
     3121 ataatcagat gaaatcaaaa atcaagccct tgtatagata catatctgtc tggcgggaag
     3181 ccataatagc ggtgaaatac agagataaag taggacctac ttgaataacc gcatttttct
     3241 gctacagcct gtccatatcc atgtcgggaa cataacatat ttacagcaac ccgcatccgt
     3301 tcctcaagta acaaattact gaacctgaga ccttcatcct tgagtttttt ctttaacaag
     3361 ctctcactca tatgcaactg tagagcaatc gcaccaagcg tccagcttga tgatatatct
     3421 gtttgaataa tcgctctgac tttgacactt atactggata cacatccact taaaaataat
     3481 gacatccgtt catctgattc aaacagcgac aggcaggcca tcataagaaa catatccgtg
     3541 gtctctccgg aaaatctctg gctggtaatt aaagccgcag ccagcgcagg attgttgggg
     3601 tccagcaaca agtaaagcgg aatgtcagtc agaggacctc ttgtcagctt atgctggctt
     3661 tccagatatt gacttactat agaatggctt atatcgacaa ttttaacttt gccataatgc
     3721 ataaggaaaa gctccctgat gcatttggtg gccagaacga ctgagccggg cttaagtgac
     3781 aacgtatcct tttcaagaaa aatattaatt ggggagcaaa ccatgataac tgaacagaca
     3841 acatccatta taattttact ttaattagca attggttagc ttaattatag ccccaaaaag
     3901 taaattctca tcaacacata agcaaatgac tgacaggtgc cgctaacacc catcagccgc
     3961 ccattaccac aaataaaaaa gccttcactg cggaaggcgt ctgtaacaac cgaactgata
     4021 atctgccaga cccgccataa caagctgggt cagtattaac tgacagcgtt cgcgtgaaag
     4081 gtacgtattc tgcgcaattt ccccgacggt cgccggttca gtgacgctta attcattaaa
     4141 caccactctg gcggtttcgg tcatatcctg ctgttttagc atgccttttc ccttttctgg
     4201 ttaacgtgac ataccaataa ctcttgtcga aaaagccagc aagctgaaag cctcgtatcc
     4261 gcaaccacca gcgcgtttaa cgtcctgtgc cgctttttgg gcgcaaaaaa cccgccaggc
     4321 ggcgggttta agctgtgtgg cgaagtaacc actcttaaca ggatattcgg atttttggaa
     4381 ttccgcaaga ctttgcatca ggctgtagca agggaagcct gctcttcata catctcacta
     4441 tcttgatctt ccatttcaag agttcggtaa tattgcagcg cggctggatt ggcgacgtac
     4501 tctatatttc catgatggtg cttcaccact tcaatgattt tttctagttc aactctgaaa
     4561 aattctttcc tcaaattgac tttatttact ctatagcgct ccaaataatc atgtaatgct
     4621 ttttccaaag ctggagcatc atcgcaggaa atcatggcat gcacatcaaa atcaaatggt
     4681 acagatgcat cacccaattc tttaactcta tccattggtt ccagacgtcg agtcatacca
     4741 actttatata cattctcacc aaatgaacca atatttgata ttatatatac gtgtccttga
     4801 cgagttaatt gcgccataga cttggcgcgt tcatactgct tatgcacatc tgcaattttc
     4861 tgttcaagct ccaatctctg ttgttccagt tcggctttat atgtcccctc agccaaagcc
     4921 aaggcttcat caatagcacg ctgctgttct tccagtcgct tctcctcttc ttctgcttca
     4981 cgttgcctac gctctagctc ttcctgacgc tgacgctctt ctttcatttg gcgcttgatt
     5041 tcattctgat attctcgttc ttcttgagca gcctgtagag ccagcacctt ttctttaaat
     5101 tctttctcta ccgcctccca atcagaatga tcctctaaat ggaagaaatc atatttagcg
     5161 attaatcctt tatacagttc acgctctttc tttatatctt gtagtttttt tcataatttt
     5221 ttaaagaaac tgatgataaa agagtttttc ttttataacg aaaggtatca tcaagtactt
     5281 tttgtatttc aacttgagct gttttgtgct gttgaaagaa ttcatcttca aaattaaatg
     5341 cataatcaac cgcttttgac aaaatatttt tacatctaac tcgatgattc tcaataagcg
     5401 cattcaattc aatgattttt tgttgggttt tatactctgc atccctaacc ttttcatcac
     5461 acttttcacg caaccataac aatttctctg agtgttcagc gttcaatttt tctatttcat
     5521 catttttagt ttttttcgaa tatttccata cccatatact acctatagct aaaccgatta
     5581 aaaaaactcc aaaatgagaa gttaaaaaat caaatatacc gcccatagaa atacctcaat
     5641 gttccatgta tccattcact tgcccattgc aagcgaatgg attttattca gcaaacaatg
     5701 aaaatcaagg gacgctttaa ctacccatga tgaagagcac catcaacatc aagcttcaca
     5761 ttcataattg ataaacaacc atcaataaaa ccttcagcct tttgtaaacg tttaagaatt
     5821 tgagtgtgcg agatgccttg tcttttggcg atatttctag cagaaatact atgtatataa
     5881 tgcagttcaa gtagtgtaca aagatatgga tcttttttct ttaacgcatt catagccgaa
     5941 ctgataatca gtccgtcatc atcactgcaa ataggtctga ctttgccaga aaaaggaagt
     6001 atgcttttaa acccagctgc tatcggtgag tagtaaacac tacctccttc attcgcagcc
     6061 cacgcccccc aacgctcaag aatcatctga atatcacgca tcagcgcagt acctcctgca
     6121 ccagtttttc aaacttacca actctggttt ccagctctgc cacacaatcc accagctcat
     6181 ccactgcttt ttgtgcacgg tgttttgcct gcatcagttc cctgagcgct ggcaccatat
     6241 cccgacgaat ggcatctttt gttacacctg ttttttccag ttgttccgcc tgtcgcaaca
     6301 tttcctgtgc ctgtttacgt aattgctcag gggtaaaagt cattgtctgg ttgttcaaaa
     6361 gaaacgctcc atcttactgc tgtcagttcg tttgttgctg tatctgcgcg gctggggctg
     6421 ttgcattggg gtggaaagaa cctgtgcgct ttcctgatcc acaggcagaa aatgtccgtt
     6481 ataaaaacgc cggtaaaccg tccccagaga accgttacgc tgtttcgtga tattgatttc
     6541 tgcgatgccc ctggcctgcg tatccgggtt gtacacttca tccctgtaaa gcatcagaat
     6601 gatgtctgca tccgcctcta tttctccgga atttttcagg tctgagttca tcggacgttt
     6661 attgggtctg gattccacac cgcgggagag ctgactcagc gcaatcaacg gaaaaccccc
     6721 ggattttgcc aggcctttaa gcccctttga gatttcaccc acggcaaggt catgacgccc
     6781 cgtggttcgg gtttttatca gtccgagata atcaaccacc accagcgccg tttccggatg
     6841 tttaatcagg tggtgtttcg ttgttgcgca tatctcgtca atggtcaggt tcgcctggtc
     6901 caccatccag atattgcgcc cggtcatccg ccccactcct tgtgagaaac gtgcccagtc
     6961 ttcgtcttca aagtgagtga cagatttcag gcgtgatacc ggcattcctc cagccgcaga
     7021 taccatgcgt tcaccaatct ggatgttcgc catttccatt gtgaacagaa gaacaccatg
     7081 cccctgctca gtcaccttgt cgatgatatc cagcgccagt tcggttttgc ccatcgaagg
     7141 acgtgcagca ataaatacca ggtcgccagg ctccatgccg cccgtttttg cgtccagttc
     7201 atcaataccg gtcatcaacg tcctggattt ctccagtccc tgattccggc attcaatgcg
     7261 ctcaactacc tccggaagca catcatcaat atgtacaggc tgaataatgc ccttttccgt
     7321 cgacaatgag gccatcatgt tctgcgcatc cttcagggca tcttcagctg cttcacaggt
     7381 atgcgcatca cgtaatttct gcagcgcctt attaagtgtt ttttctgcat cacgcaatgc
     7441 ggcattgcgc cgcaacgctg caacatagtg ctccagtgac gacttcaccc aggttttgcg
     7501 cccggtatca gtaatcaccg gagcaagttc cggcatctca ttgcacagca gcacaggatc
     7561 aatcacacct gaaacacgag cctgtctgca gatgcctgtg tagatatccc gatacgctcg
     7621 tacagaaaaa acgtccgcag gtagtgtggc cagaatatcc atcacttcag gatctgcccc
     7681 acgcagaaag aacgcgccaa tgacagcgcc ttccaaatca tcgttacgcc agactggtgt
     7741 tgtcatgcag ccacacctct gatacgagaa cgatagctgg gccagttaaa cgacaaccag
     7801 ttgcgtcctc cgtctgtgat cctgtcggca atgcgggggc tgatgaacgc ccacaactct
     7861 tccggtgaaa ggttgctgat caggatggtg ggcaagatac tttcgtaccg ggcattgata
     7921 atttcctgca aaatagccat ttcagccgcg ctgccaaact gaacaccaac ttcgtcgatg
     7981 atcagcaaat ccattgacgc ataatgctca ataacttcat ccgctgtttt ttcgctgtca
     8041 ttccgccagc agtttttcac agcacgggta aggcgcatca cgtcggtgat ctccacacta
     8101 gccagatagt tacggatgat gtgttttgcc attgataccg ccagatgatt tttcccggtt
     8161 ccgcaactac cggtcataac aagactggta ccgttctcca gcatatctgg ccagttctcc
     8221 gcatagcggc gacaggctgc aagatttctg gccgcgtcag gattaacctc cagataatta
     8281 tcaaactcgc agtcccgaaa acgcagggca attccggcgt tatcagtcag ctcttccgcc
     8341 ttgagggacg acagctccat ggtcaaatcg ttggcctcag cgatcaagca gtcagggcag
     8401 catgaaattt tttctctgtc ctcgccattg cgatcgctcc acaccagtat atgcgtatgg
     8461 tatttaccgt gtttttcgca atatccgcga ccttcacgca tcaggcagga acgataaggc
     8521 catggctttt cgcccttctg agcaaatgca atctctgccc gtaactcatc cattcgcgcc
     8581 tgtagtcttg tttgttgttc acgcaggtta aacgtcatca tcgctgtcac ctcagaatgt
     8641 caatttgtca ctggatttac cgaatttgtc agacatggca ccaaggccag acaggacatc
     8701 gacctgtcgc tgtcgcccac ctccgggagc ggctggctgt tgccagaaat cttcgaagtg
     8761 acgatcgggt ccaaagaacg tcgcagcctg cttcacgaac tgtgtgccgg tatttcctgt
     8821 agcacgtacc caggcggcat accgcttcac accatcaagc atggtctccg gttttattcc
     8881 ctccctgata cgggctttcc aggctttgaa ggctgctgac ttggaattgc caccagcacg
     8941 tttgggatat tcctgccagg cctgttcaaa ttctgatgaa tattcctgtc gggcagaacg
     9001 cgctggtgca gacgcgtcag cggatgcgcc aatagttgat tcattgactg gttctttgac
     9061 tggttcaaaa gagtgactgg ttctgggtga atctcctgca ctaccccctg gtgcaactcc
     9121 tgcaccacct ggtgaatttg ctgcactagg tagtgaatta tttgcactac cacctggtga
     9181 atcttttgca ccatcaagac gaagaagata aatattgctc gagttccctt tttcaccttt
     9241 ccgggaaact ttttttacca gtcccgattc acaaagagcc gtaatatgat tcatcacaga
     9301 acgtttgcta atctcgcact gatcagcaat atgctgatag ctgggccagc actcgccctg
     9361 atcgctggca ttatcagcca gcttaatcag aaccagtttt cgcaacggat ttcccacgcg
     9421 aattttcatc gctttcacca tcagctccat gctcataaca cacctcccag gcgtttaaac
     9481 atttttccgg actgaaacac caccaatggg taactgatgg tgtagttacg ccccagtaat
     9541 tcacacacaa ctttctggct ttcggtgctg accaggcaaa cccgcagaac gtgaccgttg
     9601 ctggtggtga accactgccc cacacagggg caacggttgt atcggtgata cagggagtta
     9661 acgatgcggc gaatcatgga cgtacctcct gcacatcagc catgagcatt ttgctggctg
     9721 tttccagcgc ctggtcggcg ctaatttgca ttacagccag tgaatgagga acgaaagccc
     9781 cagcatattc tgtttcagtg gtggcgtgct tatgcgccat atctgcgata acagagatgt
     9841 caatcagtgc atccatcagg gttttaatta ccgtaacaga ggtgtctgaa taatggttat
     9901 tacacatggc tcgcctccca ttgattacgg cgaaaagcgg tgtgactgag gctggtttct
     9961 gccttatgga atgcttcaat acagctctcg tagtaccgca ttgtgcgcag acttaaccca
    10021 agctgaagca tcatcaggcc gtcaagggtg atgtaataac cacgcaggga atcgccatac
    10081 acgtggtaag tacccggtat gaaattacgg gtgaaaaatt cgcgcgagca gttcaggtac
    10141 tcgattttgt cgacgatgtt ctggtgcatg cgcttaaagt ggcaggcaac atgcagggag
    10201 aaaataacag ctttgccgtt gacgacttcg attttcaggt atggggaagt tgggactgta
    10261 gccatgatgg cagcctccgt atgcaatgga taacttccac caccggagct gcgaaactca
    10321 tgggtggtga actgaacagg gttcgcagta ccggcgcata cggaaaccgg cgagcctttc
    10381 ggctcccctg cccagcccac cataattctg gcgtgcgtga gcgcggacga taaaaaagac
    10441 gctagcgcgt catatatcgc cgtatgcaat tccgggctgc gacccccggc acccgcttta
    10501 taaggtgcag gaacagtgta acgtcccgaa attgcagaat caatattttg gagaacaatc
    10561 atgcagcacc cgccagttct ttatcgtggg tgaattcgcc attccagttt ctcttcatgg
    10621 gtaatgcacc tctaagatac tggcgataaa tccagaccgc ccctttctgg agaagaactg
    10681 gtgtgtacga ataaaaccct tcttcataac ctgaatcgat atagtggtga cgttcggtca
    10741 gatacagatc tcgggcatat gcctttacac gccatgcggg gcttcgcgat tcaggacgct
    10801 cgtcataaag ccagttgtga gcctccagaa atgctgtgat gcgctgaaca tttactccat
    10861 taagttgctt gcagaactga acaggtgtca tgccaggctg gaataaattt tccagatgtt
    10921 cgatgtattg ggcctgacga tgaacgtaac taattgctct gtttttagcc tcatattcgt
    10981 ccgcccaggc acgggcagca gcagccggat cgctaaaatc tggtagggat gtcattgtgg
    11041 taagacagta attccccgtt ttacgaattg acggaattac ctccgatgta atccaccgtt
    11101 taaaacgtct tgcctgtttt tttcgacttt tgataatcag cgcatagagc ccggattcat
    11161 tgaccagcaa aggcttacgt cccgaaccta agtaatcctt atattcggtt ttatcttcat
    11221 catcgatgga ctgaagggag atagcagtat ttgtcagccc cagcgcgtta catatatcca
    11281 cggcaaaaaa ccatggtgtt gaatcaatga ccacactacg aactgatgac aacaattcac
    11341 cagtgcttga tttaaaatcg aaaactttga tattgttttt cacgtaatat ctccgacgat
    11401 aaacccgact ggccggtttt ttctgccggc ctttcttatt tctgccaacc aataacctga
    11461 aataacccca ttttcgggtg ataccagcga gtccctcgcg gttctgcttc ctccataacc
    11521 cgataaaaag cagccataaa cggttccaca gcaacaattg cgcgacgtga taacaatccg
    11581 tccggcgtca taaactcgtg ggtatctgta ggaatttgat aggcgttcac cagattgcgg
    11641 catttatcat ctgacaaacc ggttttcgct ttcagttggc gatatccggc atagccctca
    11701 cgaatggtgc cctttttgat ttgctcaact gtttcggcaa catggctgac tttttcttcc
    11761 acctgagtga tccgtttctg ctggcgaaca gcttcaagag ccatcgctgc aaccatttcg
    11821 atttcgctca ttggcttacg gatctgttct tccagctcgc gccagcgatc taccaggcga
    11881 gcagtgaatt cagggcagag ttgtgcgacg acaatgatgc tgtcgcgctt accttgttct
    11941 ccttcaaaca ggtaatgctc atattgaact ttaaaaccta agttattgat tctttcggaa
    12001 acctcaattt gaggagaccg gacaacgcca cctttggcta atgtttcaat agtgcgtttc
    12061 acattgtcat gacgtttacc caccagctct gcgatttcaa cgctggtcat ggatactttg
    12121 tcgttaaaaa ttgcagcgtt cattgttgcc tctttacgaa aaaccatgat taacctctag
    12181 ctttccctgt cttttccgca cttcttgtac ggtgatatta ttcagtgccg gatgcttacc
    12241 cgtgtccggc gcacgaccac acgtagccgc gtgttggtct ccattttcaa ttcagctctc
    12301 aatggaggat aaaaatgtca aatacccaac atctaaaagc ggcggatccc gttttacgcc
    12361 tcaatgcact tgaaacggtt gttcgggcga tgctgtcgag catgacaccc gagcaaataa
    12421 acaaagcgcg attgattgct actcgtggtg cccaattagc agccctagat ccattgctca
    12481 ctcctgcgaa tcgcgaagat gtagagcaca ttcaggggat ggttgattat ttattcgccc
    12541 atattcctga tcagatttga ttctttgaac cataatttca gcgatacgaa tggccgccgc
    12601 ttcgagcctg gcggcttctt tatctgcata agagctaccc atcacttctc gtcctctgtg
    12661 tgcgctaagc ttgggtgtgg aaaaagctct ggtagatcag ggcgaaattc atgcgcctga
    12721 atctttccat cgactgctgc aaccaataac ggaacaaatt caggcgaaat tttgttcttg
    12781 cagttcagcc aatcgcaaac tgttgactga gcctttccgc atcgcttggc taaggcttgt
    12841 tggctcccaa gaatctcaat agctttctct atggctttat gtttcataat cgcatctcct
    12901 attaaatgca taataacaac aggaaaagcg atattcaacg ttaaatctag aacatttatc
    12961 ggaaagccga tagactagcc aaggtagaga ggggaggaaa atcgcaatga cattttcaga
    13021 acgacttgat ttagctatgc gcaacgcaaa atttacgcaa ggcaggctgg ccaaagaagt
    13081 aggtatggct cagtccagtg tcaatcaact acttaacaaa gccaacggtt ccagaaaaac
    13141 agtcgagata gcaaaagttt tgggcgttaa cccggaatgg ctcgcatcgg gtgttggtcc
    13201 aatggaaatc aagacatctg cagactcaca ccaaatacgt aacatttcag aagactgggt
    13261 tactgattcg tatgtagtgg atgttttaga catcagatat agttgcggac ctggtagtta
    13321 taactctgat tttcctgaca ttgtcagatc tatagcgata gaaccaggat acgcatcaag
    13381 agtttttggc ggcagaccag cctcagcaat caaagcaata aacgcccatg gcgacagtat
    13441 gaaaggcaca atagatcctg aagacttggt atttgtagat gtatcagttc gcacatttaa
    13501 tggcgatggt atatacgcct ttacatactc tggaacatcg cacatcaaaa gacttcaaaa
    13561 aatcaaagat actctcacgg tgatatctga caacccagcc tacaaggatt gggctatcga
    13621 accggaagac ttagaacaac ttcacattga cggaaaagta attgtcagtt ggcccatgac
    13681 gctacaccgt tttgcataag cgaaaatcat ttaatatacc gcccactctt aagtgggctt
    13741 ttttttgaag aaaaatcgat aaaccgattg acagacaaaa tcggcaaacc tattatcatg
    13801 gcataagtat cgaactcacc aaagcaatta tagaggatga ggaaatgtca acatatatgg
    13861 ggttaccaac aaatcagcac gatgctcttg aaaatattca gcttttagta gggaccagtg
    13921 ctctactgta tagcagcaat aaccctgagt taattgagct tgccagctcc atcctagatg
    13981 taactcaaaa atatagcctc actgcggcac gttcatacaa acaaaatttt gctattaatt
    14041 cacggaatcg tcttcgcacc caacgcgaag cctgcggctt aacaacagcc gaactcgcca
    14101 ggctgctcgc tctcgatgaa gaaatcatca tccagtggga gagcggagag tatgaaccaa
    14161 ccatcagtat gcttatccca ctggcaaatg ttcttggctg tgatccgatg tggctgttaa
    14221 ccggtaaacc aacagcagga gatacttgcg catgaaaaag ttcgaaaaca taaccattct
    14281 ctgtgttgat gagtttgatt atacaaacca ggaatttctc ccggaggtta taaaagcaat
    14341 agatgttgcc gatatagtga ttagaggaaa gagaattgtc aaaaacaggc tcgcatgcac
    14401 ttcaggagca atgacagaaa caacctcaca acaagatgat tatgaaggca tttgtctgga
    14461 gcctgattca tttgcggtaa atgtttatca tttattgcat gcaacacagg tattacatat
    14521 gtccagtaat cacgaaacaa aaacacttgg cagcgaaatt ctgaattttg catgtgaata
    14581 tgcaaaatct gctgccgaaa aagaattagc gcaataacaa taaatatgcc ctgaacgttt
    14641 attacggttt tatcgccggg gattgttaca acctttattc gcaggagatt atgttatgac
    14701 tttcctgaaa cataaggcat cgtataaaac tgcctgcctc attgcacaac atggagattc
    14761 ttatcttcat atagccaacc tgtatttgcg caaagcatat gggagataag tcaatggaag
    14821 aaaaacgaca aaacataaca cataaaaaag taagagtgtt gctaaccatt gaaaatggtg
    14881 aagtaattta ctcaaaacat ctgttggata atgaattcgt tggctgcatg gatacatttc
    14941 tgtggatggc aaaaagagcg ggttacacga ttattccacc agcaaaggag caaacattat
    15001 gaatcattca gagttccgac cagaagttac gccacatggc ataaaaattg gcaatacaac
    15061 cattgattat gttgaggccg tacagcggct taatgatggt gaatacgatt atccaaattc
    15121 acgcggttta agcattctcc agtgcctggc agaagcctat gagtccggga ttttagaaaa
    15181 ctgttctatc catatgaagg ttgcacagtg gcgctggctg tatgtaacgg tatttattaa
    15241 tgaagaggag tataagaacg gcactattga tatccctaac gataacggaa ctacagatcg
    15301 cgcagttatt tataagggga agcatggttg catgagtata tacccaggac cacttcgcat
    15361 tgccctgcaa aaccatgtcg aatggggatt tattgaaaaa tatggcgaag ctgaaggcat
    15421 ggggcgagtt ctgtttctct atcaaaaaat gctcatcgca gatcctgata atggtttcat
    15481 tgtctctgct atggggcgcg aagggcttga actccttctg gatgaaatga ttaacgacct
    15541 gaatactcat agtatgccag aagcgccagt gacacattaa atattaagaa gaatataatt
    15601 cttccgtttt ttactaaccg tttgtatgaa aagcaaccgt gaattaaaca gagtaaaact
    15661 gattttaatc cttgccacag tactgacact aactgaaatc attattctct ttattgtgct
    15721 gtcagtcggt taaaaatatc gggataccac agaccaatga gactgtattt cacaatagta
    15781 attttactgg caattatcgc atgtatttac ggattacttg ttccgttcct tatatccatg
    15841 aaggatacga tagcagttat ttctggcttt gcactggcgt ttctgacccc gccctgcatt
    15901 tatgccattt acaagggtct ttctttcact aaggataaaa gatgaaaaaa attatttttg
    15961 ctttagccat tgttctgccg actattggcc ttgtcggttg cgatcgcgtt gaaccaggta
    16021 atgttggcat caaggtaaat aaactgggcg acgacaaagg cgtcggtgag gtggtcggtg
    16081 ttggtcgcta ctggactggc tggaatactg aagtttacat cttccccacc ttcaagcaaa
    16141 tgaagaccta cgatgaaccg ttcagtttcc agatgagcga cggtacaacc atcgggtatc
    16201 acattggcgt ggcctacaaa gttgatccat ccaaagttac cacggtgttt cagacctacc
    16261 gcaaaggcgt ggatgacatt accgacaccg acctgcgtca gaagatcgcc gacgcactta
    16321 accgactggc cagcaaaatg accaccgaca aatttatcga cggcggcaag tctgagctgc
    16381 tggatgctgc tcttaaagac attcaggaag aaatgacgcc catcggtatt caggtaatga
    16441 acctctcata tgtgggtaag ccggagtacc cgccaaccgt tatcgacagc attaatgcca
    16501 aagtcacggc gaaccagaaa acactgcagc gcgagcagga agttaaacag cgcgaagcgg
    16561 aagccaacat gttgcgcgcg gaagctgccg gacaggctga tgcgattcgc acaaaagccc
    16621 aggctgaagc cgatgctatt cgtttacgcg gtgaagctct gcgccagaac cccggtgtta
    16681 tggagctgga agccatcaat aaatggaacg gcacactgcc gcaatacatg accagcggcg
    16741 ccagtacacc atttatccag gttaaataac ttatatgccc ggcaggtcgc cgggctaagg
    16801 gaaaagcaga tgaacaccca taatgcccaa ccgcaaataa tgaactatga cccgaatctg
    16861 acgtcatgcg gacgcatgac aaaacaaacc gttcgattaa ctttcgggtt atgggaatac
    16921 cgcgaaacat tcgaagttac tgtcggcggc aatctgaccg gactggatgt tatcagttgc
    16981 gctattgaaa gcctgtacgc aacactgcct tatgaagaag tcgaggatga gcgcacaggg
    17041 ggaacagata tcatggccac cattaatatt ggcgaactga tatgtcagga tgaagacctg
    17101 tccggagaac cctggcttgc cgggatgctt atctcagcag aaattatcag tattgaaccc
    17161 gctacaaaca tacgactctg aagttctcac tattcagaga gcaggagaaa aaatgttcgc
    17221 tttgattaat cagggacaac tgtataccga cagtgccggt tacccggtaa aaattattcg
    17281 ctgcataaat aacactgtgt tgtacagaag aatggatggg cgaacacagt cggtaaaaat
    17341 aaacgatttt aatgaactgt ttgaacggct cgatcaccag gaataccgac aaattctggc
    17401 agaaacagaa caggaaactc atctgaaaaa attacgggcc atgaaaagga aataaagaat
    17461 gaataaagcg tttgagctat gggtacgcca gcgttacggc aatcgctatg acctgacgcg
    17521 agatgttgac ggtttctact gctgtgaaat tgtgaaacga atgtttgaag tgtggtgcca
    17581 ctgccgtggg ctgagtgttg tgtgaggtaa ttcatgggca atgtgattca actggctccc
    17641 aatgaatggg tttgtgaaag cgttcttatc gcaattaccg ggctcaaacc aggcacaatt
    17701 ctccgggccc ggaaagaatg ctggatggta ggaagagagt atattcacgt atcgcctgac
    17761 ggtaatccaa aaccttccag cgaatgtatg tataacagaa aagcaatcga tgcctgggtc
    17821 gcttcaatga aaaacaaaca acctgggtga tttaatacca tgaaatatgt aagctcatat
    17881 cgctcttggg cgtctggagg tatcgatgga taaagtcaaa tatccaacag gcgtcgaaaa
    17941 ccacggtggc acattacgca tctggtttaa ttttaaaggt aaacgtgtca gggaaaatct
    18001 tggtgtccct gacactgcca agaacaggaa gatcgccgga gagctgcgga catctgtatg
    18061 ttttgccatt cgtacaggaa actttgatta cgctgcgcag ttccctgact ctcctaacct
    18121 caaggctttt ggggtaaata aaaaagaaat tacggtgaaa gaacttgaaa aaaagtggct
    18181 ggatctgaaa cgcatggaaa tctctgcaaa tgcattcaat cgctatgagt ccgttgcaag
    18241 gacaatgatt ccgaaaattg gaggtagcag actggtgtca acggtgacca aagaggaatt
    18301 gctgtatatc aggaaagatt tgctgaccgg atatcagaat tcaacgaaag acaaagcacc
    18361 agtaagagga cggagcgtcg ttactgtaaa ttattatatg acgacaatcg ctggaatgtt
    18421 tcagtttgct gcagatcacg gttacttaga agcaaatccc ttcgagggaa ttaagcctct
    18481 taaaaaagcc agggcagagc cagatccgct aactcgtgac gaatttattc gcctgataga
    18541 tgcttgccgg catcagcaga taaaaaacct gtggtcattg gctgtgtaca caggaatgcg
    18601 tcatggtgag ctggtctccc tggcctggga agatatcgat ctgaaagcag gaacaattac
    18661 tatcaggcgc aattatacga aact
//
LOCUS       DAZFRE010000094        18642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFRE010000094 DAZFRE010000000
VERSION     DAZFRE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.094"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1954)
                     /locus_tag="ACHKUI_003850"
     CDS             complement(<1..1954)
                     /locus_tag="ACHKUI_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6599953.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGGVNEASG
                     STKTLSGVLNGVAGHIDAVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQTTLD
                     NKQQSALTKTERELALSRLKGEEKERA"
     gene            complement(2002..2211)
                     /locus_tag="ACHKUI_003851"
     CDS             complement(2002..2211)
                     /locus_tag="ACHKUI_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6599954.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALNFAAQS"
     gene            complement(2307..2681)
                     /locus_tag="ACHKUI_003852"
     CDS             complement(2307..2681)
                     /locus_tag="ACHKUI_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6599955.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2699..3412)
                     /locus_tag="ACHKUI_003853"
     CDS             complement(2699..3412)
                     /locus_tag="ACHKUI_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6599956.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTLSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSFECTVTVSAAA"
     gene            complement(3478..3822)
                     /locus_tag="ACHKUI_003854"
     CDS             complement(3478..3822)
                     /locus_tag="ACHKUI_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6599957.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3819..4265)
                     /locus_tag="ACHKUI_003855"
     CDS             complement(3819..4265)
                     /locus_tag="ACHKUI_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6599958.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4262..4612)
                     /locus_tag="ACHKUI_003856"
     CDS             complement(4262..4612)
                     /locus_tag="ACHKUI_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6599959.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDTT
                     GGRLEILCRLGGEK"
     gene            complement(4626..4952)
                     /locus_tag="ACHKUI_003857"
     CDS             complement(4626..4952)
                     /locus_tag="ACHKUI_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6599960.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(4949..7371)
                     /locus_tag="ACHKUI_003858"
                     /pseudo
     CDS             complement(4949..7371)
                     /locus_tag="ACHKUI_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(7317..7538)
                     /locus_tag="ACHKUI_003859"
     CDS             complement(7317..7538)
                     /locus_tag="ACHKUI_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6599961.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            complement(7583..9520)
                     /locus_tag="ACHKUI_003860"
     CDS             complement(7583..9520)
                     /locus_tag="ACHKUI_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6599962.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRIREGLEITATLVKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIQTIKSFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGHGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGQGKARPLTKFDFESITFSHAKVAAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDTDAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            complement(9585..11246)
                     /locus_tag="ACHKUI_003861"
     CDS             complement(9585..11246)
                     /locus_tag="ACHKUI_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6599963.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
     gene            complement(11243..11806)
                     /locus_tag="ACHKUI_003862"
     CDS             complement(11243..11806)
                     /locus_tag="ACHKUI_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6599964.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(12098..12463)
                     /locus_tag="ACHKUI_003863"
     CDS             complement(12098..12463)
                     /locus_tag="ACHKUI_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6599965.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSDRADDIIGL"
     gene            12505..12705
                     /locus_tag="ACHKUI_003864"
     CDS             12505..12705
                     /locus_tag="ACHKUI_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6599966.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPTSMTNNEK"
     gene            complement(12904..13138)
                     /locus_tag="ACHKUI_003865"
                     /pseudo
     CDS             complement(12904..13138)
                     /locus_tag="ACHKUI_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13135..13629)
                     /locus_tag="ACHKUI_003866"
     CDS             complement(13135..13629)
                     /locus_tag="ACHKUI_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6599967.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKNVREL
                     KLANAAITDMQMRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCTKLAFYY
                     PEDT"
     gene            complement(13928..14461)
                     /locus_tag="ACHKUI_003867"
     CDS             complement(13928..14461)
                     /locus_tag="ACHKUI_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6599968.1"
                     /translation="MSRKLHYGLSVAVLALIATGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKLPLTEPQK
                     AGIASFCPYNIGPSKCFTSTFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(14466..14681)
                     /locus_tag="ACHKUI_003868"
     CDS             complement(14466..14681)
                     /locus_tag="ACHKUI_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6599969.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVTPSQWAAIGVLGS
                     LCLGFLTYLTNLYFKIKEDRRKAARGE"
     gene            complement(14758..15048)
                     /locus_tag="ACHKUI_003869"
     CDS             complement(14758..15048)
                     /locus_tag="ACHKUI_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6599970.1"
                     /translation="MTDIKQLVTAEAVKEVIRSEEVRSVLKQKLRQNLEERLDAEVDS
                     ILDELLGAQPEPSPELLPEPQAEDVTTENGDIQPESPVTDMTDTQPEPGTML"
     gene            complement(15074..15268)
                     /locus_tag="ACHKUI_003870"
     CDS             complement(15074..15268)
                     /locus_tag="ACHKUI_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6599971.1"
                     /translation="MTFINQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SATQSAGAKTEAPLIPEQPS"
     gene            complement(15409..17382)
                     /locus_tag="ACHKUI_003871"
     CDS             complement(15409..17382)
                     /locus_tag="ACHKUI_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6599972.1"
                     /translation="MAFKHYDVVRASSPSDLAGKLTQKLKEGWQPYGSPIISSAGYGA
                     EFIQAIVAEGDMTSPVVSPGGDGTRTVVVEPEYYFVIALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDLSRFSHPKASAEQYGCVG
                     QGLHIAKKLLPFMPENAGILLVPCCRGGSAFTAGADGTFSESSGASDKSERWGVDKPL
                     YKDLLTRTQAALKANPKNILLCVVWMQGEFDLKQGAYASQPAMFDAMVEKYRSDLAGV
                     SGQCAGASPSLVPWICGDTTYYWKETYSAQYDAVYGAYKTKAGKNIFFVPFMMDDNGQ
                     KVGTNEPSEDPDIPAIGYYGSGGRTDAKSWTTSDRKTHFGSWARRGIISDRLATAILL
                     HVGRVADFVSGKVTKAAVSSGGGVSQPAQESGDTQPESVVPTKIQSVLAYDANAENAD
                     LASQGWTLTDVKNTLVSDSGSGKKALRLEKPENGLAQKKTWKVSHAIDAGKGKELFDN
                     GGEITLRFKIPDDVSLNASANQYSAGIYWRGSGWPGAESEEGYIAAFYLQTDKTNINV
                     MYHANTTSQRLGSYGPFDHDWHTLTFRFPGGGSLNVTPVLDNAAGKPFTLTRWTNAAF
                     EANALAITDITGNAATYPVLIESLTVGVNAVAA"
     gene            complement(17993..>18642)
                     /locus_tag="ACHKUI_003872"
     CDS             complement(17993..>18642)
                     /locus_tag="ACHKUI_003872"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6599973.1"
                     /translation="ELEAYLIAAGLSDYQLTEKDYLILEHFKNIEFSGFPVTELFSVC
                     NTGNILSRDIIENSGDVPYLCASRDNNSVSSYIAYDDSLLEKGNCIFIGGKTFVVTYQ
                     ERDFFSNDSHNLRLYVNNEDGRTKFAYLGIISCIYRSISHKYSWGDSVSNRKIKDDVI
                     WLPVKNKSPDFCYINDLISVVHKLVIKDVVQYAERKMNAYRQAITSRGYDDSGVS"
BASE COUNT         4102 a         5740 c         4424 g         4376 t
ORIGIN      
        1 gggcacgctc tttttcttcc cctttcagtc tggacagggc cagctcacgc tctgtttttg
       61 tcagggcgct ctgctgttta ttatccagag tggtctgtgg cagccgtaac ggcacattca
      121 ccagtccctg ccgctgctga agcagttcat tccccagccc cagcagacgg ttgaattccg
      181 tatgctgacc gttcataacc agcatggact ggtacacctt attctgctct gccgcctgct
      241 gacgaattaa cgccacacga cggtcttcca gcccggcaag cacatcctga atggactgcg
      301 ctttttcctg catctgtgcc agacgggact gctcaacggc aagctgctct gttgcctgag
      361 aaagcccttc cgttacggtc ttcaccgatg tcagatggtt tatcatgaat ccgtcaccgg
      421 ttgtccagcc agggttagcc agaacatact gatatccagc gattttttcc tgcagggatt
      481 tcacccggct ggcctgttca tcaatcagcc ggttctgctc tgtcagcgcc gcccgtgttc
      541 gtccttcatt atctgaggct tcaggcaaag acattgacgg cgttttatgc gcgatttcat
      601 ctatcgtcag tgcatactgg cgcgctgact ccctggcctg ctcctgattc tggtacagcg
      661 tataccatgc tgctgccccc agcatcacca gtccgggtac gccaccaacc agtcccaacg
      721 caccagtcat cagacgtgaa cccaccgccg ttgtactgtt cagcgcattc tgggctgcgg
      781 ttctggcagc aatatttctg ttcaggcgtt cctgtgtggc cgccagacgg gcttctgcag
      841 caatctgcat ctccgtcccg cgggctgccg ccacagcctg ctgtgcacgg tacacggctg
      901 cccttgcccg cgccgtggca atctgcgtcc ccctgagctg tgcttccgcc agtgccactt
      961 cattacgtgc tgccgtcaca agtcctgccg tggcagacac cgctccggag gccatattgc
     1021 caaagtaccg ggcaaccccg acggcaacca gcgcccccgc tgctgttgcc actgcatcaa
     1081 tatgtccggc cacaccattc agcacgccgg agagcgtttt cgtcgagccg ctggcttcat
     1141 tcacaccgcc cacccaggcc ataaaggcgt tttccacctt cgtgatacta ccggaaaccg
     1201 tttccggcat ggccgcatat tcatcacgta atatccccag ctggctgatt aacgcaggga
     1261 ccactttatc cgctgtcagt tttccgtcat ccgccattgc cttcagatct ttacgggcca
     1321 cacccatccc cgcagcaagc gcgcggatca cccggtcgcc attttcgtta acagcattaa
     1381 attcttcgcc acgcagtaca ccctgtgcca gtgcctggct gaactgggtg atcaccgaac
     1441 cggattccgc aactccagcc cctgacagtt tcagtcctgt cgaaatggcc tccgtcacct
     1501 tcagcacatc atcagcactg taaccatatt cacgcattga ggctgccgag cgggcaaaca
     1561 gggccgcatt atctgaaaat gccgtgcccg tccgctggct gatatccatc agcactttct
     1621 gtgatgacga aaattcatcg gatgactgcg acgcctgttt cagtcgggca ttcacggaac
     1681 tccattcatc ggccagagaa atcaggtgcc cggtggcaaa ggcaccggca aatgcccccg
     1741 ccgttccggc agctgaagcg cggatttccg tcaactggct gttcagctca gccagggcgc
     1801 gtcgctgctc ccgggcgact gcggcagcct gacgcccgcc attctgcagg gtccgataat
     1861 attcactgcc catgcgggaa gcccgctgga tctccgactg gaatgactgc gaatttgccg
     1921 aaattttgat aatcagttca cgtaacgtcg ccattcacct ttctccgggc gtaaaaaaac
     1981 cgcctcagcg gttctcatca ttcatgactg tgctgcaaag ttcagcgcgt cttccagcgc
     2041 cgcaaacgga tccacctccg gcttatcctc atcctcgccc cagcagagca tggcgtcctt
     2101 cagtgcaaca ttcatcccct gtgccccgaa aaccgctttc acgatctgtg cattacggat
     2161 atccccgcgc tcatcaccca gcggggatac cctgtcgaac tccatccaca tcatcgcctc
     2221 gctcgcactc aggctgtgcc gcagttcgga taaggtgcgc cccagacgga gcgcaagtcg
     2281 catcagaaag cgaatttccg ggcgggctac ttttttctgg ccgactctgc atcagcgatc
     2341 agttccagtg cctgacgcag caaccgggca tgtaccggac catagacggc cagcacctgc
     2401 tcacggtcgt ccggagtgaa cacccgctgc agatccgtat cacacaggac atcgcagaac
     2461 agcgtcacat ccgcttccag gttacggcgg gttttcgcca ccaccgacag ggtatcgtca
     2521 tcctctccat caccattgag cacttcctgc cacagatacc aggcctctgc cgaaggctcc
     2581 cgcagcacca cgctgacatt accccattcc ggcaccttca ccgttttatg acgaaaccct
     2641 gacagtctgg ccagcgccag cgttttcaga tcctttttca tgatgaccca tccccttatc
     2701 aggcggctgc gctcactgtc acggtgcatt caaaagacgt cacactctgt gctttctctg
     2761 ccgaatcggt caccacgcag gtatatttcc ccgcatcagc ggactgcgca cctggcttac
     2821 ttaaggtgtc tgtcgtctgc ccgtcaaccg gctgaccatc cttcttccag gcgtatttat
     2881 acggcggcgt tcccccgttg gcactgactg acattgtcag cagcgcaccg gtattcacgg
     2941 taagtgtttt atccagattt ttcacaaacg ccagcggtac cacaaaggac accggtttgc
     3001 ctttcagacg cagtgagaac gttgcagcca ccacgccgtt ggtaccggat gaccaggtgt
     3061 gctgacgcac ttccgccagg aatttaaagc ccttaccgga cggaaacagc accttaaacg
     3121 catacaacgc gtcattgtca taggcatcac gcagggcgtt ctgggcctga ttcagataaa
     3181 aattacccga catggaaatc tcagacgacg cccccagacc gttgatgttc tcctgctctg
     3241 tggagcagag cgtggtcaca tcaatatcct gtttctgacc ggcggtgaac tggacttcct
     3301 tgatggtgca gtccaggcgc agatattccg ccttatccat agtttcagca gtcgccgggg
     3361 cagatgaaat catcacctgc gtcagctgtg agcgttcata caaagcagac attctgcctc
     3421 ctgataataa aaaacccgca cgcggcgggg tatggttttg tagaaaaaaa gaaaaagtca
     3481 caccgtgacc tgaaactcca gggttgcacg gtaacagcgg ttttccggaa tatagtcctg
     3541 catttcactg acggatcccg gggccagcag cattatggct tcacgggcgt cctgacgtat
     3601 ctgacgcgcc tgcgtcacag tcccggcata aacgtctatc tgcaccgaca ctgaggactc
     3661 cgcctgcccg cccatcacgt ccgctgacac cgatgaaatc aggctgaaaa ccacccacgg
     3721 aagcgccacc gacggcctgc catccagcag ggggaccaca tacgggtaca cctgcccgcc
     3781 ggcaagatgc gccagatgag gatacaaatc cgcctccgtc atcgtctcag tacctcatca
     3841 atggcccggt tcatccgcgc aatcgccacc tgagctgcct gttcactgcg cacatcaaac
     3901 gccgggcgca caaacgggtg cggtggcata ttcacggtcc ccatttccac aaaccgccag
     3961 tagaaagcat tgcgcgggtt atccgccttc atggtgttat cgctgttacc ggtgtccgga
     4021 ttaacaccac ggatatggac accggattcc atcccgccat cgcgggagcg ccgggaaagg
     4081 atcaccacat tgcggcgcag ttttcccctg cgtaccggtg cccgtgacac cacttcttct
     4141 ttcagcacat tcgcacccgc acgggttgcc tcacgcagca cccggttatt ttccgcacca
     4201 ctcagaagct gcaaatcgcg gctgatgtcc tccagccccg aaaaatccag cagggtttcg
     4261 atcatttttc ccctcccagc cgacagagaa tttccagacg cccgccggtc gtatccggca
     4321 cgggcagccc gacaacgttc aggatccggt cacgccatgg accactcagc acatgaagtc
     4381 gtgacgctgc tgtgatttcc cggccggact gaccgcgcac ccagatgcgg atttccgcct
     4441 gagccatttc cgcaccggac tgcatccgct cccggctgct cctgccccgg atatccgcat
     4501 gaattttccc gcatgacacc cactcttccg tcatttctcc ggcagcatta cgggttaaca
     4561 ccgggttcag aacacttatc atctgtgtca gacgacctgc agatattgcc atcccccccc
     4621 cctcctcata acaccgtcgg acaacgcaaa tcgtaaatca gcacggacac agaaaacggc
     4681 agttccccct gcacgaggtc ttcccgctcc gcaagatccg gattccggta caacatcccg
     4741 gtcaggcgca tggcagcccc cttcatccgg gttaatgcct cgcccgggat cagttcaccg
     4801 tcctcacgga ttaccttatc ccggctgccc tgaatgtagg ccagcagcac agctgtagcc
     4861 tgacgaacct tgtccatcag catgtcatca tccgcgtcat ggtcaacacg cagatgagcc
     4921 ttgatttctt ccagtgtcag taatgctgtc actttccacc tcctgcatcc cgccctcgtt
     4981 ttgcagccag ggtccagcct gatgaatgag cttctccggg tttatcaccg gtcatgctgt
     5041 tgcagtgcca caacgagccc ccccacgtca ccgtatcgcc ggggtggtag gtttcgccgg
     5101 ttctgaatac accgcgatag agcatcaccg gcagggaaaa tgttttttcc gtacactggc
     5161 cactgctctg ccggaccacc acagtgaacg accgttcgcc ggtcatgctg acgtcaatat
     5221 cggccacccc gtcaaccagg cattcccatc cccgcatccc gtgcgttttt tcatacgccc
     5281 gccagagtcc gccctggtgt gtggcatacg tgccccgggg aaaggatttt tgctcatcaa
     5341 tcgccgggaa tatttccagt gccgtagcat cacgcccgtc ctgtggagcc ggcagggcac
     5401 tcaccgcatc cagaaccgcc ttctgcagaa cttccggatc gtagtcacga ccgtcgcgcg
     5461 gaacggggat atgactcacg gcatccgtca ccatctgctc gagcatcgga cgcacatcat
     5521 ccggtgtgat actttttcca tctgccggta ccggaagttt tccgacagcg tcattcaccg
     5581 ccctcagcag cacctccgga tcataatcac gaccatcgcg cggagcaggg atatgactca
     5641 ctgcgtcctt caccatctgc tcgatcatcg ggcgtacatc atccgcagtg acacttttac
     5701 cgtccgccgg taccggtatt ttcgcgaccg catcattcac cgcctgctgc agcacctccg
     5761 gatcataatc acgaccatcg cgcggtaccg gaatggcccc cacggcgtca ttcatcatcg
     5821 cctgcagaac agggcgcacc tcatccaccg tcacatgctt ctgtaacaga gcagacaggg
     5881 aagccagttt ctcttcaaac gcccccgact gtgcggccat cttctcctca aatgtgcgct
     5941 gtaaatccgc cagcaccgtg gcgaattctt cacccagcgc acggataatg gacagttcac
     6001 gctctgtcat tttcgcagta tccccctgaa catcgccttc accgcatcac gctctgtttc
     6061 gcttatggcc ttattaccgt cagatgcgcc gtcaggcagc tgtgatgaaa ctgttttccc
     6121 ggtcgacgcg aacggatcct cacgggcatc acgacgggac agcgcctcca gactgtagtt
     6181 ctgctgctga agatacagtg catcaccgcc ggccagaggc ggcaggttct cccgtttacg
     6241 ggcctcattg ggcgtgagaa gcgtattttt caccgcatcc cccagcgttt tcatgcgccg
     6301 ctcactgtcc attctcagca gcgtggtgac atcaaattct gtactctcgt tttcccccgt
     6361 ttccagcgcc tcatccagta acagttcaat ggactcaatc agcgtctgca ggcactggga
     6421 ataatactgc tgctccagcg cctccacgtt gtcactggaa ggcggttgtc ccacgccaat
     6481 cttgtaggcc gggacacgga acaccgaaca gacaatttca gcggtcatct tcagttgttc
     6541 caccgtctgc gcatccaccg gtgaaaacgt cgtggggttg tattttaccc cgttgctcag
     6601 aatggccgtt ttccccgcat tttcgcctgt atacccgctg tcccagttgc tcttcagttt
     6661 tttcgcattt tcttccgtaa tactgccggg gatctcaatc accccggacg gcctgccgcc
     6721 atttctgaaa aaagacgtcg aattttcctg aatatgatgc ccctgcgtgg ccgccagccc
     6781 ggcagcatac accggcggca gccccacaag cggatgaaaa aaacagttaa accggtcgtg
     6841 gatcacttcc ctggcaggca ccgtcaccgc ctcagtgatc ccgcagttcc ggtccggtgt
     6901 aatgcgatag aacacctcgc cgtcatccgc caccagaggt tcaacccggc tccagtccag
     6961 aatacgcagt tctttgatct gcccccggga gttacggatt ttcaacacca ccgtattgcc
     7021 gtgacgcagt ttggcgttca gccacagttc aaaaaactgg atacgattct gctgtgcatt
     7081 gggacgacga cagagacggg caatatcccc ctgtcgtttt tcacggcgga tcccctgtgt
     7141 atcggtctgc atcaggcgca gccgcatttt ggcgatatcc tgggatatca gcgaaatgca
     7201 agaaaacacc gcgtgaaagg acaaaacggt ttccggatcg gctttcacac cctgctgcca
     7261 ggcaccggca aaaggctcag ccaccgcctg aaacaggctg gtccagccca cctcttttac
     7321 atcacgtcct gatttctggt tttttcgggt tcgccgcaaa aggttccaca ttcgccatgc
     7381 tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca ctgtgatgta ctccgccttc
     7441 cccaggcgaa ccagcacctc cgcacacggc tgtgccacat cacggatatc cccggcccgg
     7501 gcatcatgcg tgccctgcag atactggatt tttgccatca gttactgcgg gaagctcgcg
     7561 cctcccgccc tcctcatcag actcagccgc cggacgcagt tccgtagttc acaccggtga
     7621 tcaccgccac tgccgcggta cggcgacgac gccagttgat ccagcgctcc gcacggatgg
     7681 ccacgctgcc ggtctggaac atggagacca gctccaccgg tgacggtgtg ctgctgtcgc
     7741 tggtcggttc agactgcatc tccagtgacg cttcacggga catatccacc gccacaccgc
     7801 cgtcatcagc cagataaata tccggcgcat tcaccagcac cagctggtca cccacgtact
     7861 gggagacaat caccggcagg ccctggaagg tgccgccaag cagggtcatg tccggatatt
     7921 ccttctgacc cagcgcattt ttacgcatgg acagcgccag ggcatttgtg ctggacatca
     7981 gccagaccgc accggtgggc tgcaggtttg ctgccacaaa ctgaccaaac gcggcttccg
     8041 catccgtatc cgggttaccg gttgatgccg tgcccttcac atcatgggtg atggacgcag
     8101 gggaaacatc ggccaccgcc gcttttttcg ggtccacaaa gtctgtgtcc agacgcgcca
     8161 ccaccgcttc cgccagtgca ttacggacca gcgcatcggc ggcaggactg gaaaaacgga
     8221 tcagctcttc cgtcagtacc gcaatagccg ccaccttcgc atgactgaag gtgatggatt
     8281 caaaatcaaa cttcgtcagg ggtctggcct taccctgccc cacccagccg gcagcaccac
     8341 cggacacctg ggcatgcacg cgaatattga acggcacctg acgaagtgcc gggatcccgc
     8401 catgaccaaa tcgcccgata atggtctgcg gacgcagata atcaataaag tcctgcgcat
     8461 attcctgata ttcagacagg ctgcctgccc actgtgggtc cgtggtggtc cctgccccca
     8521 ccgccgactt caggacatga tgcagacggc tgtcatccgg atactgacga cgggccactt
     8581 ccagggcttc agagcggaca cctttagccg cggccagtga tttggcaaag cgggcgaaac
     8641 caatcccctt ctccagtttc tgctcaacac ggatcaccgg cgctgaagcc accgtggcca
     8701 cattcccgtt accggcctgt ttcaccggct gtgccgtggc ggccttactg gtttccagtt
     8761 cacgcagacg cttcaggtgc gcatccacct gacggatttc cgctgcggtg ttgtcgtagt
     8821 gctcttcctc ttccacatcc agtgtgcggc cttcctctgc ggctttggtc atgacctcct
     8881 caagggaggc tgccagcgcc gcacgcttgt tttcaaaact tttaatctgt tcaccagtat
     8941 tcattgctga cttttcctta tgaaaagagg ttattgactg tgccgaagcg ccggcagaag
     9001 atgcgatttt caccaccggt ttccggttgc cggacgcggc agaaaacggg cggtcgaaag
     9061 atttaatggt ctggatggtg cattccgcat tcgcgggcac ggtgacggca gacacctcca
     9121 tcagctccca gcgcagaaaa tgcagtccgc ctccgtccag ataagtgtat tcatggggcc
     9181 ggaagcccac agaaagcccc ctgaccagcc cggtcttaat ggcagcccat gcctcatcca
     9241 gccgggcagc cagttgcgac ggcatatccg gtacgggctt caccagtgtt gccgtgattt
     9301 ccagcccttc cctgatccga cgcaccgtac actggcctac agggcgggaa tggtcatgct
     9361 gccagagaaa cgggatcgta ctgccaaact ccgccccctc cggctccagg atgtcaccat
     9421 cccgatccgg agaaggcgtt gacgcaatcc cggtgatcac ccgttcatcc tcactgaagg
     9481 atttcaccgt cagcagggaa caggcccgtt taagagtcac atcagcctcc tgaaaataaa
     9541 aaaaaccgcc gcagcggttc atgatggtta cagggtgagc agggttatat gaaaaaaacc
     9601 tcatacgctt tctttttcgg ttccggattc agggacatca gggacaccgc attgaagagc
     9661 gccatcagcg ggtcaatttt tccccgtccg ctggcctgtt tggtaataag gatggcgtta
     9721 cctttaggct ccacccgggc attgccgaca caccaggcca tcaggggctg gccaccatgc
     9781 accagtaccc cttcagccag tttgcgctcg gtggttttga tggccccgcc cagcttccag
     9841 ccctggctta tccccaccac aattccgtcg gggatcccgg cttccgccag tgaatccaga
     9901 atctgcccca cccctgacgg gtcaataccg atatggtcca gtaactcagc ctcatgaatg
     9961 cgacgcacat attccgccac ttccgccgtg tcatccccga cacgccggac aatggtcata
    10021 tctccacagg caacaagatc ctgaaaccgg gacgcctcgc tcttccgtcg gaccaccgcg
    10081 gtttcatgcg cccaggcatg gccccagccc agccattcgc gggtctcccg gtcacgccca
    10141 atcacataca tccccagcag atcatccagc cctccgccgt caatccccac cgtcaccaca
    10201 tcagcacgac gcaggatatc gtccaggctg atacaacggc cctgctcttc ccagaaatca
    10261 gcccccgccc agcggtcaga gcgcagggca agaccaattt ccacattggc gtgttttgac
    10321 atgaaccccc ggaatgtctc ttcaccggct tcccgggctt tacggtactc ccggtacaga
    10381 aaggcctcat ccactgaata gccgagattc ggattgacca tggcgaggtt ttccatcagc
    10441 aggtgagccc cgctttccac catttcagga gggtgttcaa atatcaccgg cagaaagtgc
    10501 ggatcatgaa ttttgccgtc gcgcacatcc cgggcgtact gcagtttctg tctgaacacc
    10561 ccggcaggcg gttcattcga ctgggtggtc gtatacacca caaacccttc cgggcgggag
    10621 gcaaggccgc ctatggcttc acgtaacatg tcctccgcct tgcactgctt gccaaacagc
    10681 cacaactcat caatcagcgt acccacggac ttgataccgg acaccgtatt cggatcggca
    10741 gccaccacct tcagggtggt gtctgtcacc ctgtgggtga tggtccggat atgggtctgt
    10801 acctggcaga ggtcatccag atcatcgtca cgtcgtacca tatcccgggc agggttgaag
    10861 gcgttggccg ccacctccac agtcggggcc agaatcgtgt aacccgccgc ctgccgccag
    10921 ttcagtaaca gtgcagtcat catgatcccc gcggccagcg tggacttcga gtttttcttg
    10981 gggataagga taaaaacttc cttgatatgg cgtacaccgg tctgcgcatc gtaggagcca
    11041 aacagggccg ccaccaggtc aaacacccac ggtgcacagg actccccgaa cgtcgggcta
    11101 ccaggtgcat ccacaatccg cagttgttta aaaatcgcca gggcatgtgc ggcctcgtcc
    11161 ggataaatcg gatccggaat aatcgacagc ccctttttca ggcgctctgc ccagtccggg
    11221 caggctgtgc tccatacagg tatcatccgt tgccctcatt atcgttattc accaccagtc
    11281 ggggtggcgg tggcaccgca aaacggttag ccgctttttt cgcggcatca ccttttgccg
    11341 attttttccc ggtatcccct tttttatggt gcgtgaactg cgccagacgc caggccgcat
    11401 ccagtgccag tttcggatca atgcagaggt tttccaccag gatccgcccc atggctttca
    11461 ccggatcggg aagaccatcc tccatatatt caataccagg agacatcacc gcggacggtg
    11521 gcatctccgg attattttcg tccggctgag gtattgcagc cgcctcacgg cgacggggtt
    11581 tatcctcctg ctctgatttt ttctgccggt aaacaggaac ctcatccacc tccaccgtct
    11641 cgcattgttt acgggctata aacgcaagca cctccggatc ttttgccagc tgcgagcctt
    11701 taaccctggc ggtcttcgcc gaataaccgg cggcaatggc tgacgctgtt ttgtttttcc
    11761 cggacatgag cgccagcgca aattttcgtt tttgcgttgt cagcacagcc tcctcccggg
    11821 tccagaacgc actcagccgg gtatggttca gcccattttt ccccggcgtc tcatgccgca
    11881 aatgttaact gctgcctggt taacatttgc tgaaaaagcc tgttaacatt ttttccgcac
    11941 aacaaactga ataataaaga taaaaaccgc aaaaatgccc gggcagccag ttaacatgtt
    12001 aactgccctg aaacgggaat tttttctctg cgtgagaggg ggcgcggtgt ccaaagcgat
    12061 cgttttttac gccggatgat accccccccg ggtcgggtta cagtccgatg atgtcgtccg
    12121 ctctgtcact acctccggac acctccggca gcgtcgggtc cggcatacca cccgccgctt
    12181 cacgagcaga cttttgtcga tggcattcgg tacagagcgt ccagagattc gtctcctcat
    12241 taccaccacc gaactgaagt gcaattcggt gatcgagttc actgtcacag aggtcaacca
    12301 cacgaccaca gatacagcac tgcccggcgt ccctcagcca gatatgacgc ttgagggaaa
    12361 cacgtgcact gccactgaca cgacgctgtt cccccttcag aatattcacc cgtcgggtat
    12421 tcagtgtttt gattctgctc tggagtgtac gaagctcagc catgtaaaat ccccgtcata
    12481 tggcaatcag taaaggaaat aaatatgtca tcgaaaaacc ggacccgcag aaccacaacc
    12541 cgcaatatcc gtttccccaa tcacataatt gaacaaatca acatagccct tgagcataaa
    12601 gggtccggta acttttcagc gtgggttatt gaagcctgca ggagaaggct ggcaacagat
    12661 gcaacgcatc tgcgcccgac cagcatgaca aataacgaga aatgaacgtt cggtttcttc
    12721 caccatcgca ccggacaggc gactatgagg ggacaacgcc gcgctccgtt aacgcggtaa
    12781 accccggtgt gtatcgtttt tgattatccc cgcacactcg cgcagaggag tctccctgtc
    12841 gggctgcggt ctctgttaat gcaggaatac ggcgacaata ccgcgcatgg ataataaggt
    12901 cgctcaacac actggctgta atgcagcgga taccatgcgg catttagcgg catttagcgg
    12961 cattcatcgt acactccacg gttagctctt cattcgtggc attcacctga aaggtccggg
    13021 agtgtaattg cgtacattta ccactgaacg aaccttcaac aagaacacga ccacgctgca
    13081 aaatacggaa cggaattgtt ccctgaaaag gttctacggt tacccgtaat ttcttcatgt
    13141 atcctccgga taataaaaag ccagcttagt gcactgagtg cggatatatt cctgcgcccc
    13201 ttccagctgc ttctgcattg tcatcagccg ttctctgagg atgaaataat cccgttcagc
    13261 ggtgtctgcc agttgggggc cggttgcatt atccacgcgg gcggtgccgg tggcttcacg
    13321 cacgggacct ggacaggtgg cgttgatacg caggcgctta cgaccagcgg caacgtcagc
    13381 gcgaagagtt tcattttcag ctctcgcatc ggctaattcc ctcgagtatt ttgcatcgag
    13441 cgcagcaaca tcacgctgac gcatctgcat gtcagtaatt gccgcgttcg ccagcttcag
    13501 ttctctgaca tttttgtcgc gctgggcttt gtaggttatg gcgttatcac ggtaatgatt
    13561 cagccccaga ctaagcgcac cacaggccac cagcagggca atgatgacca cgcacagtac
    13621 gcggttcatt tcaccaccag cgtatctgac cgatgaaata accggaggcc ataatcacaa
    13681 acaccagcca gataagaatg aacttccagg tggataattt ttcagccatc actcgaatct
    13741 cccgaatcag tttgctaaaa tcaaacacac tttctccttt gacttttcca gagtcaggaa
    13801 acacaaaacc ccgcttgcag ccaacaaacg gggtttttac ttttattcac ttagtttttg
    13861 tcagttcgca ggatttcgtg ttatccgtcc gtgtgagcaa accgcatttt tcagcaaaat
    13921 attctgctta tctgtcaatt ccccagcacg ccagcgcgct ctcctggtca cgccgcgata
    13981 cctgcccgta acagttattt gagcgattcc gacagtccct gccgccgtca tatatccatc
    14041 ggcggatttc agcacatgca cctttccggt ctccggcgtt cagtttccgg taaaacgttg
    14101 aggtgaagca tttcgaaggc ccgatgttgt acggacagaa tgacgcgatg cccgctttct
    14161 gcggttcagt cagtggcaat ttgatgtttt tctccaccca tgccagcgcc ttatcacgtt
    14221 caatggcatt aacctgagcg catttttcct tcgacaactt catgcccggg acgacaggtt
    14281 tgccatccac catgatggca ccacggcaga tggtccagat ccccgcacca tcacggtatg
    14341 ccgtggtgtg gttaccttct ttttcgtcaa gaaactggtc gaggatttca ggcgcagaag
    14401 cacctgtggc aatcagcgcc agaacggcta ccgataaacc atagtggagt ttcctgctca
    14461 tcagcttact ctccccgcgc agctttgcgc cggtcttctt taatcttgaa atacagattc
    14521 gtcaggtatg tcaggaaccc cagacacagg ctccccaaaa ccccgatagc agcccactgt
    14581 gacggagtaa cctgatccaa ccactgtaga aaccagtaac ccgcactgcc cgcagaagta
    14641 ccataggcta tgcccgttga tattttttcc atctgataca tatccccgcc ccgacagacc
    14701 tgtgctaccg gaaataaaaa aggccatcag tgttttactg atggccctgc cccgccgtta
    14761 cagcattgtg cccggttcgg gttgtgtgtc tgtcatatcc gtcaccggtg actccggctg
    14821 aatatcacca ttttccgtgg tgacatcttc cgcctgtggt tccggaagca gttccggaga
    14881 tggttccggc tgtgcaccaa gcaattcatc cagaattgaa tccacttctg catcaagacg
    14941 ctcttccaga ttctggcgga gtttttgttt caggacgctt ctgacttctt cagagcgaat
    15001 gacttccttc actgcctcag cagtgaccag ttgttttata tctgtcatga gattttctcg
    15061 ttgaaagggg ttattaagaa ggttgttccg gaatgagtgg ggcttctgtt tttgctccgg
    15121 ctgactgagt ggcgctgatt ttctcagcgg cccttttgtc aatctgtctg cgccagaagt
    15181 cgcgtactgc cctgtatcca cccgaaagca gatacaacac acagaccacc gtacagaagt
    15241 acagcattaa ctggttgatg aatgtcatgg tttattaccg ttactgttga caatgagaac
    15301 tgttttcatt tagaaatgat tgatgtcgaa agcatccttt cgttagattc tccattggga
    15361 ttacctccgc cagtctccat tcctgttgct ggcggttttt ttcgtctgtt atgctgctac
    15421 ggcattcaca ccaacagtaa gactttcaat cagcacaggg taagttgccg catttccggt
    15481 aatatccgta attgccagcg cgttggcctc aaaggctgca ttcgtccatc tggtcagcgt
    15541 aaacggtttt cctgctgcat tatcaagcac cggcgtgacg ttcagcgagc caccacccgg
    15601 gaagcggaaa gtcagcgtgt gccagtcgtg atcgaacgga ccataactac caagccgctg
    15661 tgatgtggtg tttgcatgat acatcacatt gatgtttgtt ttatcagtct gcagatagaa
    15721 ggccgcaata taaccctcct ctgattccgc tcccggccat ccactaccgc gccagtaaat
    15781 acccgcagaa tattgatttg cagaagcatt gagagataca tcatcgggga ttttaaagcg
    15841 cagcgtgatt tcaccgccgt tatcgaacag ctccttcccc ttaccggcat caattgcgtg
    15901 cgatactttc caggtctttt tctgtgcaag accattttct ggtttttcaa gacgaagggc
    15961 ttttttacct gaaccactgt cactgaccag cgtgtttttg acatctgtca gcgtccagcc
    16021 ctgcgaagcc agatcggcat tttccgcatt cgcatcataa gccagcactg actgtatctt
    16081 tgtcgggaca acggattcag gctgagtatc gccactctcc tgtgctggtt gcgatacgcc
    16141 tccgcctgaa gaaaccgcgg cttttgtcac tttcccggaa acaaaatctg ccactctgcc
    16201 cacatgcaga agaatggctg ttgccagacg gtcggaaata attccgcgac gcgcccatga
    16261 accaaagtgg gttttacgat cggatgttgt ccagctcttc gcatccgttc gtccaccgga
    16321 accataatac ccgatagccg gaatatccgg gtcttctgac ggttcattgg tcccgacttt
    16381 ctgaccattg tcatccatca taaacggcac aaagaagata tttttaccgg ctttggtttt
    16441 gtatgcgcca tagacggcat cgtattgcgc tgaatacgtc tccttccagt agtacgtcgt
    16501 gtcaccacaa atccagggca ccagagaagg agaagcacca gcgcactgac cggatactcc
    16561 cgccaggtca gaacggtatt tttccaccat ggcatcaaac atggcgggct gactggcgta
    16621 cgctccctgt tttaaatcaa actcgccctg catccagaca acacaaagca gtatgttttt
    16681 ggggtttgcc ttcagcgcgg cctgagtgcg tgtcagcagg tctttataca acggcttatc
    16741 aacgccccat cgttcagatt tatccgacgc tcctgaactt tcactgaacg ttccgtcagc
    16801 accggctgta aacgcagagc caccacgaca gcacggcact aacaaaatac ccgcattttc
    16861 cggcataaag ggcaacagct ttttggcaat atgcaatccc tgtccgacac acccatactg
    16921 ctctgcgctg gctttcggat gagaaaaacg gctcaaatcc tgcacatcat gcaggcagtg
    16981 gtccgccgga atgatgtcgt tatatgcaca ggccgcacca cccggtgtca ccgtactgcg
    17041 acgcgccagc tgtttaatac gcgggtccgg acggtcatat gtctccggca gcggcaggcc
    17101 ttcaccatac gccatgccgt ttgactgtcc ggccagcgca atgacaaagt aatattctgg
    17161 ttcaacaaca acagtccggg taccatctcc ccccggtgaa actaccggag aggtcatatc
    17221 cccttcagcg actatagcct ggataaattc agcaccataa cccgcgcttg agataatcgg
    17281 actaccatag ggttgccagc cttccttcag tttttgcgtc agttttcctg cgaggtctga
    17341 cggcgatgat gccctgacca cgtcatagtg tttaaatgcc atgaatcctc ccggccggga
    17401 tagtgggttg aatcagataa ggactgactg aagtctgaaa atacaaaaga caactgccag
    17461 taaaaacgaa aaggccgcgc agttgcgcag tctcatcacc atcgggtatt attcacacgg
    17521 tgaataatcg aaactcactt tctttgtttt attccttgcc gctcgcgcct cccggcgcgg
    17581 gctttttttg catgtaaaaa ggcccctgcg atgaggggcc tgagagtatt acctggtttc
    17641 agttaactgc atagtgccgg gtgcctcccg gtgagttcag cccggtgcca ccgaacccgc
    17701 gtctttcaca ttgcctgtga cattaaaaat gccagcccgc accagccgcc ccgccgctga
    17761 gggggattca ccatgcgttg cgatttttaa caaattctcc gtcagtcaga caatcatcaa
    17821 cttgctgaat tgtgaggtat ttaaaaattt ccccgattaa ctgataccct gctaatcacc
    17881 tgttgttttc tttttcagca acaaaaaagc ccgctcatcg gcggattcgg ctgcgtggca
    17941 atgtaaccat tcttatcatg atatgaggat ttttacgatt gtaaaatgtt ttttaactga
    18001 caccagaatc atcataaccg cgacttgtta tagcttgcct gtatgcgttc attttgcgtt
    18061 ctgcgtactg gacaacatct ttaatgacaa gcttatgaac aactgaaata aggtcattta
    18121 tataacaaaa gtcaggtgac ttatttttca ctggcaacca aataacatcg tccttaattt
    18181 ttctattact aacactatcc ccccatgaat atttatgtga aatagaccta taaatacatg
    18241 aaataatgcc caaataagca aactttgtcc ttccatcctc attgttaaca tagagtcgca
    18301 aattatgact gtcgtttgaa aaaaagtctc gctcctgata agttacaacg aatgtcttac
    18361 caccaataaa aatacagttc cctttttcca agagtgagtc atcataggcg atataagaac
    18421 taacagagtt attatcacgg ctagcacaaa gataaggaac atccccacta ttttctatga
    18481 tgtctctaga caatatgttt cctgtattgc acactgagaa taactcagta acaggaaagc
    18541 ctgaaaattc aatattttta aaatgttcca ggataagata atctttttcc gttagttggt
    18601 aatcactcag cccagcagct atcaggtatg cttccagctc tt
//
LOCUS       DAZFRE010000095        18451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFRE010000095 DAZFRE010000000
VERSION     DAZFRE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.095"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(81..1289)
                     /locus_tag="ACHKUI_003873"
     CDS             complement(81..1289)
                     /locus_tag="ACHKUI_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6599974.1"
                     /translation="MTDGGGMHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLAD
                     ARVRRDETRKLLANGVDPGDKKKNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVL
                     KSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSG
                     LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFI
                     RSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGSKMRTTHLVPLSTQALAILK
                     QIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLVE
                     SGLWSRDAVERQMSHMARNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFE
                     YAKINNPLKQ"
     gene            complement(1532..1616)
                     /locus_tag="ACHKUI_003874"
     tRNA            complement(1532..1616)
                     /locus_tag="ACHKUI_003874"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:Leu,seq:caa)
     gene            1812..2831
                     /gene="ahr"
                     /locus_tag="ACHKUI_003875"
     CDS             1812..2831
                     /gene="ahr"
                     /locus_tag="ACHKUI_003875"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6599975.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(2835..3398)
                     /gene="idnK"
                     /locus_tag="ACHKUI_003876"
     CDS             complement(2835..3398)
                     /gene="idnK"
                     /locus_tag="ACHKUI_003876"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6599976.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            3615..4646
                     /gene="idnD"
                     /locus_tag="ACHKUI_003877"
     CDS             3615..4646
                     /gene="idnD"
                     /locus_tag="ACHKUI_003877"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO6599977.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSKLHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            4670..5434
                     /gene="idnO"
                     /locus_tag="ACHKUI_003878"
     CDS             4670..5434
                     /gene="idnO"
                     /locus_tag="ACHKUI_003878"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO6599978.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            5499..6818
                     /gene="idnT"
                     /locus_tag="ACHKUI_003879"
     CDS             5499..6818
                     /gene="idnT"
                     /locus_tag="ACHKUI_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO6599979.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6885..7883
                     /gene="idnR"
                     /locus_tag="ACHKUI_003880"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6885..7883
                     /gene="idnR"
                     /locus_tag="ACHKUI_003880"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO6599980.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7961..9463
                     /gene="yjgR"
                     /locus_tag="ACHKUI_003881"
     CDS             7961..9463
                     /gene="yjgR"
                     /locus_tag="ACHKUI_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6599981.1"
                     /translation="MSKPLLIARTLDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAAEGTASEKLLARLKNIGVNDWQPHTNPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILRAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNTPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9624..10706)
                     /gene="lptG"
                     /locus_tag="ACHKUI_003882"
     CDS             complement(9624..10706)
                     /gene="lptG"
                     /locus_tag="ACHKUI_003882"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6599982.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(10706..11806)
                     /gene="lptF"
                     /locus_tag="ACHKUI_003883"
     CDS             complement(10706..11806)
                     /gene="lptF"
                     /locus_tag="ACHKUI_003883"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6599983.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAINVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(11869..11919)
                     /gene="ytgA"
                     /locus_tag="ACHKUI_003884"
     CDS             complement(11869..11919)
                     /gene="ytgA"
                     /locus_tag="ACHKUI_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6599984.1"
                     /translation="MSIIILATISTTQENK"
     gene            12073..13584
                     /gene="pepA"
                     /locus_tag="ACHKUI_003885"
     CDS             12073..13584
                     /gene="pepA"
                     /locus_tag="ACHKUI_003885"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6599985.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13938..14381
                     /gene="holC"
                     /locus_tag="ACHKUI_003886"
     CDS             13938..14381
                     /gene="holC"
                     /locus_tag="ACHKUI_003886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6599986.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            14381..17236
                     /gene="valS"
                     /locus_tag="ACHKUI_003887"
     CDS             14381..17236
                     /gene="valS"
                     /locus_tag="ACHKUI_003887"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6599987.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTDAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(17292..>18451)
                     /gene="yjgN"
                     /locus_tag="ACHKUI_003888"
     CDS             complement(17292..>18451)
                     /gene="yjgN"
                     /locus_tag="ACHKUI_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO6599988.1"
                     /translation="SFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCRRYLYAN
                     MEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLLIFMA
                     AKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSS
                     SSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAI
                     LAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFG
                     IAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYP
                     LLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         4716 a         4579 c         4696 g         4460 t
ORIGIN      
        1 aatacatcag ttaataccgt ttgtcttttt taacaaagaa aataatccta gaataattcc
       61 cgggcatttg cccgggatga ttactgtttt aatggattat taatctttgc atattcaaat
      121 ggactgataa acctttctct gtttacatcc agaaaatcgg cccaccactg tagcatcaat
      181 cgccgttctt ccagatgctc tgctttatgg atatacgcgg ccctcactga atttcgcgcc
      241 atgtggctca tctgacgttc aacagcatca cgagaccaca gacctgattc gaccaatgaa
      301 ctacaggcca ttgttcgaaa gccatgacca caaacctcta cttttgtatc ataccccatg
      361 acccgtaacg cactatttac cgtattctca ctcatgggtt tgtgcgaatc gtgatcacca
      421 ataaatatca agtcatgggc cccataaaac tgttttatct gctttaaaat tgcaagagct
      481 tgcgttgaaa gaggcactag atgcgttgta cgcatttttg agcctctatg ggaatgtttc
      541 actccaggaa taggctcccg ctccggtggg atagtccata tagacgcttc gaaatcgatc
      601 tctgaccaac gagcaaaacg cagctcactg gaccgaataa agatcagcaa agtgagttct
      661 atcgcccatc gggttagcgg cctaccagta tagctatcta tttttgtaag caactcaggg
      721 atgcgcttta attcaagcgc gggacgatgt tgtcgattac aggaagcaac cgccccagcc
      781 atctcttgtg ccgggttata atcaattaac ccactttgca ctgcatagcg catgatggct
      841 gtagtgcgct gctgaagacg agcggccact tcaagacgtc cagacatttc tacggcctta
      901 ataggtgcta ataaatctcg agtttttaac tcagcgatat tacgttcacc aagcgctgca
      961 aaaagattat cttcaagact ttttagcaca cgatgggcgt gatcttcaga ccacttttta
     1021 ttggtgccat gccactcaat cgcgacttct ttaaaggttc gtgctttact ctgttcaacc
     1081 ttatcatttt tctttttgtc tcccggatcg acgccattcg caagcagctt acgcgtctcg
     1141 tcacgacgta ctctggcatc cgctagtgtg atttcaggat aaaccccaag tgccagcatt
     1201 ttttgctttc cctcataacg gtactgcaaa cgccagtact tagaaccatt tggatggaca
     1261 agcagatgca taccgccacc atcagtcagc taatattgct tatccgttgg cttggctgct
     1321 ctgactttga tatctgttaa tgccatcgta ttccctccct gttggtacaa gcattatcga
     1381 accggaaata ccatcatctg taccaacata tgttggtaga tgtacgttga tatcggttga
     1441 ccttgagaga gttaatatag cgggaaagat agataaacac tggatttcag gcataaaaaa
     1501 agacctcagt tgaggtctat ttacatactt ttggtgccga aggccggact cgaaccggca
     1561 cgtatttcta cggttgattt tgaatcaact gcgtctaccg atttcgccac ttcggcactg
     1621 aagaggtatg cggaaaacgt tgtggattat acctgtcgca cgccaccatg caagcgccag
     1681 cttgctcgca cctcgctaag tgctgaaatt ttcagcattg catacagcga tgtgtaacct
     1741 ttgtcacact ccaggcaccc cgccctgcca tgctctacac ttcccaaaca acaccagaga
     1801 aggaccaaaa aatgtcgatg ataaaaagct atgccgcaaa agaagcgggc ggcgaactgg
     1861 aagtttatga gtacgatccc ggtgagctga ggccacaaga tgttgaagtg caggtggatt
     1921 actgcgggat ctgccattcc gatctgtcga tgatcgataa cgaatgggga ttttcacaat
     1981 atccgctggt tgccgggcat gaggtgattg ggcgcgtggt ggcactcggg agcgccgcgc
     2041 aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg cgtagctgtg
     2101 gtcactgcga cgcctgtatt agcggtaatc agatcaactg cgagcaaggt gcggtgccga
     2161 cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa tgggtgattc
     2221 cactgccaga aaatattgat atcgagtccg ccgggccgct gttgtgcggc ggtatcacgg
     2281 tctttaaacc actgttgatg caccatatca ctgctaccag ccgcgttggg gtaattggta
     2341 ttggcgggct ggggcatatc gctataaaac ttctgcacgc aatgggatgc gaggtgacag
     2401 cctttagttc aaatccggcg aaagagcagg aagtactggc gatgggtgcc gataaagtgg
     2461 tgaatagccg cgatccgcag gcactgaaaa ctctggcggg gcagtttgat ctcattatca
     2521 atactgtgaa cgtcagcctc gactggcagc cttattttga ggcgctgacg tatggcggta
     2581 atttccacac cgtcggtgcg gttctcacgc cgttgccggt tccggccttt acgctgattg
     2641 cgggcgatcg cagtgtctct ggctctgcta ccggtacacc ttatgagttg cgtaagctga
     2701 tgcgctttgc cgcccgcagc aaggttgcgc cgactaccga actgttcccg atgtcgaaaa
     2761 ttaacgacgc catccagcat gtgcgcgacg gtaaggcgcg ttaccgcgtg gtgttgaaag
     2821 ccgatttttg aaaatcattc gcagcgctga tctgaggcgc tgccctcttt cgcacatatt
     2881 ctgttttgtc gtatcgccag cacagcctgc cgacattgtt cggtgacatt ggcaatatca
     2941 tggttgatat caatgcgcac aatatcctgt tcatctgctt gtggacgctc cagcgcctca
     3001 aactgacttt ttagtaacgc taccggcata aaatgcccag cccgacgctg cattcgcgcg
     3061 agaatagttt cataatcgcc atctaaccag aggaaatgaa catgggggct acccttgcgt
     3121 aaaatatcac gatactgttt ttttaatgat gaacagacaa taaatcctgt ttcattcttt
     3181 ttataaagac tgtatgaagc atcatttaag cgttccagcc agggaagtcg atcttcatca
     3241 gataatggaa tgccttgcga cattttatct atatttttgg ctggatgaag atcgtcacca
     3301 tcaataaatt tcgcagacaa taacgcggca accttgctac caattaatgt tttaccactc
     3361 cctgatacgc ccatcaaaat aaagctttca cccgccattt aaaatctcct gaatcatctt
     3421 acctgctgcg atactacgcc tggtggcgta ataatttcac gtttttttat ttctgctgat
     3481 aagaattaca aggcacatca cgttatgcgt aacatagtaa tgtaacaatt ttctaacgtg
     3541 atcttcatca caaataatga caattaaaac cgcttaaatg cttccgaggt gtctacctga
     3601 ccagtgagga atttatgcaa gtgaaaacac agtcctgcgt tgttgcgggc aagaaaactg
     3661 ttgccgttac cgagcagacg atagattgga ataataatgg aacattagta caaataaccc
     3721 gaggtggaat ttgcggttcc gatttacatt attatcagga aggaaaagta ggtaatttca
     3781 tgataaaggc accgatggtg ttaggtcatg aagttatcgg taaagttatt catagcgact
     3841 catcaaaatt acatgaaggg caaacggtag ccattaatcc gtctaaaccg tgcggtcact
     3901 gcaaatactg cattgaacat aacgagaatc agtgtacaga gatgcgtttt tttggcagtg
     3961 ccatgtattt ccctcatgtt gatggtggct ttacccgtta taaaatggtc gaaacgtcgc
     4021 aatgtgtccc ttatccggcc aaagctgacg aaaaggttat ggcttttgcc gaacctttag
     4081 ccgtcgcgat tcatgccgca catcaggccg gcgagttaca gggcaagcga gtatttattt
     4141 ctggtgttgg gcccattggc tgcctgattg tcagtgctgt gaaaacactg ggggccgcgg
     4201 aaattgtctg tgccgatgtg agtccccgtt ccctttcgct gggcaaagag atgggggcgg
     4261 atgtgctcgt aaacccacaa aacgacgaca tggatcactg gaaagcggaa aaaggctatt
     4321 tcgatgtcag ctttgaagtg tccggtcatc cttcatcagt gaatacctgt ctggaggtca
     4381 ctcgtgcacg cggcgtaatg gtgcaggtag gtatgggagg cgcgatggca gaattcccaa
     4441 tgatgacatt gattggtaag gagatttcac tcaaaggctc tttccgtttt accagcgaat
     4501 ttaataccgc agtgtcatgg ctggcgaatg gcgttatcaa tccactgcct ttactgagtg
     4561 ctgaatatcc cttcacagac ctggaagagg cgctacgttt cgccggtgat aaaacccagg
     4621 cagcaaaagt ccagcttgtt ttctaagaga taaataaagg aataatacaa tgaacgatct
     4681 attttcactg gcaggaaaaa atatcttgat taccggttca gcacagggca ttggcttttt
     4741 actggcgacc ggcctgggta aatatggcgc acaaataatt attaatgata ttactgccga
     4801 acgcgcagaa cttgctgtag aaaaactcca ccaggagggt attcaggccg ttgccgcacc
     4861 ttttaatgtt actcataaac atgaaattga tgccgccgtt gaacatatcg aaaaggacat
     4921 cggccccatt gatgtgctgg tgaataacgc cggtatccag cgccgtcatc cttttactga
     4981 gttccctgaa caagagtgga atgatgtgat cgcagtaaac cagaccgccg tgttcctggt
     5041 gtcgcaagcg gtaactcgtc acatggttga acgcaaggca ggtaaagtta ttaatatttg
     5101 ctcgatgcaa agcgaactgg gacgtgacac catcacccct tatgccgcat cgaaaggggc
     5161 ggtaaaaatg ctcacccgcg gcatgtgcgt cgagctggcg cgccacaata ttcaggtcaa
     5221 cggtattgcg ccgggctatt tcaaaacaga aatgactaaa gcactggttg aggacgaagc
     5281 cttcaccgcc tggttgtgca aacggacccc cgccgcacgc tggggagatc cgcaggaact
     5341 gattggtgct gcggtgttcc tttcttcaaa agcctctgat ttcgtaaacg gccacctgtt
     5401 gtttgttgat ggcggcatgt tagtggctgt ttaacagcta ctaccctacg tgcagcgcga
     5461 aagcgctgct tttctggcac taataagaga taaagactat gccattaatc attattgcgg
     5521 caggcgtcgc gctgcttctt atcctgatga tcggctttaa agttaacggc tttattgccc
     5581 tcgttctggt cgctgccgtc gtcggatttg ccgaagggat ggatgcacag gccgtcctgc
     5641 actctataca aaatggtatc ggcagcacac tcggcgggct ggcaatgatc ctcggtttcg
     5701 gggccatgtt aggcaagctg atttctgata cgggcgcggc acaacgtatc gccactacgc
     5761 tgattgctac ttttggtaaa aaacgcgtgc aatgggcgct agtgatcacc ggtctggttg
     5821 tgggcctcgc catgtttttt gaagtgggtt ttgtcctgct gttgccgttg gtatttacca
     5881 tcgtggcatc atctggatta cccctgttgt atgttggcgt accgatggta gcagcgctct
     5941 ctgtaaccca ctgttttctg ccgccacatc cagggcctac tgccatcgcg actatctttg
     6001 aggctaatct cggaacgact ttactgtatg gatttatcat taccattccg acagttattg
     6061 tcgcaggacc gctgttttct aaactgctaa ctcgctttga gaaagcacca ccggaaggct
     6121 tatttaatcc tcatctgttt agcgaagagg agatgccctc cttctggaac agtatttttg
     6181 ctgcagtgat cccggtcatc ctgatggcta tcgccgccgt ttgtgaaatt acgttaccga
     6241 aaactaacac cgtgcgcctc ttctttgaat ttgtcggtaa ccctgccgtt gcgctgttta
     6301 ttgccattgt tattgcgatt ttcacactgg gccgacgtaa tggacgcacc atcgagcaaa
     6361 tcatggatat cattggggat tctataggcg ctatcgcgat gattgtgttt attatcgctg
     6421 gcggcggcgc gtttaagcag gtattagtag atagcggtgt cgggcagtat atttcacact
     6481 taatgaccgg aactacgctt tcgccgttat tgatgtgctg gactgttgcg gcgctgttgc
     6541 gtatcgctct gggctctgcc accgtcgcgg ccattaccac cgcgggtgtg gtgttgccga
     6601 ttatcaacgt tacccatgcc gatcccgctt taatggtact ggcaaccggt gcgggcagcg
     6661 tgatcgcgtc acacgtaaac gaccctggct tctggctatt taaagggtat tttaatctga
     6721 cggttggtga aacgttgcgt acctggacgg tgatggaaac ccttatttct attatgggtt
     6781 tgctgggcgt gttagccatt aacgccgtat tgcactgaaa gaataaaata ttgtgatgta
     6841 gtttcggggg aagattgaag attcccccga cgttgggaga agttatgcgc aatcacagaa
     6901 tttctttaca ggatatcgct acgctggctg gcgtaacaaa aatgaccgtg agtcgttata
     6961 tccgctcgcc gaaaaaggtg gcaaaggaaa caggcgagcg catcgccaaa attatggaag
     7021 agatcaatta tatccctaat cgcgctcccg gcatgttgtt gaatgcacaa agttataccc
     7081 tcggtatctt gattccgtca tttcaaaatc aactcttcgc tgacattctc gcgggaatag
     7141 aatccgtcac ctctgtgcat aattatcaga cccttattgc taattacaat tacgatcgcg
     7201 attccgaaga agagtcggtg attaacctgc tctcttataa cattgacggg attattcttt
     7261 cggaaaaata tcacaccatc aggacggtga aatttctgcg ttcagccacc attccggtcg
     7321 tggaattgat ggatgtacag ggagaacggc tggatatgga ggtcggtttt gataatcggc
     7381 aggctgcttt tgacatggtg tgtaccatgc tggataagcg agtcaggcgc aaaattctgt
     7441 atctcggttc caaagatgac acccgcgatg aacagcgtta tcaggggtat tgcgatgcga
     7501 tgatgctgca taatctttcc ccattacgca tgaatccacg cgccatctca tccattcatt
     7561 taggaatgca gttaatgcgt gatgccctta gcgcaaaccc ggatttagac ggtgtatttt
     7621 gcaccaatga cgatattgcg atgggtgcgc ttctgctgtg ccgcgagcga aacctcgccg
     7681 tgccagaaca aatttccatt gcgggttttc atgggctgga gattggcagg caaatgatcc
     7741 caagcctcgc cagcgtaatt actccccgtt ttgatattgg tcggatggcc gcccaaatgt
     7801 tgctgagcaa aattaagaat aacgatcaca accacaacac tgttgacctc gggtatcaga
     7861 tttatcacgg taacacgctt taggttattt tctcttcatt acacatattc tctttgccgg
     7921 acgttgccta tactccaggc aggaaactgg aggaaatctc atgagcaaac ccctgttaat
     7981 tgcccgcacg ctggacacag aactgttttt actgccggga atggctaacc gtcacgggct
     8041 gattactggc gcaacgggga caggtaaaac tgtcacgctg caaaaactgg cggaatctct
     8101 gtcagaaatc ggcgttccgg tgtttatggc cgatgtaaaa ggtgatttaa ccggggttgc
     8161 ggcagaaggt acggcgtcgg aaaaactgct cgcaaggctt aaaaatatcg gcgtaaatga
     8221 ctggcaacct catactaatc cggtggtggt gtgggatatc tttggcgaga aaggccaccc
     8281 ggtgcgggcg acggtttcgg atctggggcc gctgttgctg gcacgactgt tgaatctcaa
     8341 cgatgtgcaa tctggcgtgc tgaatattat cttccgcatt gcggacgatc agggattgtt
     8401 gctgctcgac tttaaagatt tgcgggcgat tacccagtac atcggcgata acgccaaatc
     8461 cttccagaat cagtacggaa atatcagtag cgcatcggtt ggtgccatcc agcgcggatt
     8521 actgtcgctg gaacagcaag gcgcagcaca cttctttggt gagccgatgc tggatatcaa
     8581 agactggatg cgcaccgatg ccaacggtaa aggcgttatc aatatccttc gcgccgaaaa
     8641 actctaccag atgccgaaac tgtacgccgc cagcctgctg tggatgcttt cagagttata
     8701 tgaacaattg ccggaagcgg gcgatctgga gaaaccaaaa ctggtgtttt tcttcgacga
     8761 agcgcatctg ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat
     8821 aaggcttatt cgctcaaaag gcgtgggcgt ctggttcgtt tcgcaaaacc cgtcagatat
     8881 cccggataac gtgctcgggc agctcggtaa tcgcgttcaa cacgctttgc gtgcttttac
     8941 gcctaaagat cagaaagcag tgaaggcagc ggcgcaaacc atgcgggcca atccgacgtt
     9001 tgataccgaa aaggcaatcc aggaactggg gacgggcgaa gcgttaatct cgtttctcga
     9061 tgcgaaagga agtccttctg tggtggaacg ggcgatggtg attgcgcctt gttcgcggat
     9121 ggggccggtg acggaagatg agcgcaatgg cttgattaat cactccccgg tgtatggcaa
     9181 gtatgaagat gacgtggacc gggaatccgc ctatgagatg ttgcaaaaag gctttcaggc
     9241 cagtatcgag cagcaaaata atactcccgc gaaaggaaaa gaggtggcgg tggatgacgg
     9301 tattctcggt ggattgaaag atattttgtt cggcactacc ggaccacgcg gcgggaagaa
     9361 agatggtgtg gtgcaaacaa tggcaaaaag cgccgcccgc caggtgacga atcagattgt
     9421 gcgcgggatg ttggggagtt tgctgggggg gagaagaagg taagccaggt gcgatttgtc
     9481 tttgcctgat gcgacgctgg cgcgccttat catgccaaca ccactgcaat atattgaatt
     9541 ttcatgattt ttcagacatg aacgaagcac actttgctaa caataataaa gggagctttc
     9601 gctcccttta ttcgttcatc aggttacgat tttctcatta acagccacag gctgattaag
     9661 aagaagctgg cacttggcaa cagtgcgccg atgatcggcg ggatgccgta aaccaacgtc
     9721 agcgggccga agatctggtc cagtacgtag aagacaaaac cgaagctaat accagtgacc
     9781 acacgcacgc ccatcggtac gctacgcagt gggccaaaga tgaacgacag cgccatcagc
     9841 atcatcaccg ccacggatag cggctggaag attttgctcc acatgttgag ctgataacgt
     9901 ccggcatcct gaccgctcga cttcagatac ttcacatagt tgtgcaaacc gctgatagag
     9961 agtgcatccg ggtccagcgc caccacgccc agtttgtctg gcgtgaggtt ggttttccag
    10021 gtgccgctca ccgtctgcga accggtgatc tgtttcggat tggtcaaatc agattcatca
    10081 acctgcgaca gacgccagac tttatgttcc gggtcaaact tcgcggtagc ggcatagcgt
    10141 acggattgca gacgacgatt ctcgttaaag gcataaatac tgatgccacc taactcttcg
    10201 tcacctttaa cccgctcaat gtagacgaag ttgttgccat ctttcgccca taaaccttgc
    10261 tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg cgccatctgc
    10321 tcgccctgcg gcgcgaccca ttcaccaatc gccatcgtca gcaagaccag tggaatggcg
    10381 gttttcatca ccgacagcgc tacctgcata cgggtaaaac cagaagcctg catcaccacc
    10441 agttcgctgc gctgcgccag catcccaaga ccaagcaacg ccccaagcag agccgccatc
    10501 gggaagaaga tttgcacatc tttcggcacg ctcagcaagg tatacattcc tgcgcctaac
    10561 gcgtcgtaac tcccctgccc ggcttttttc agctgatcga caaacttgat aatgccggac
    10621 agcgacacca gcatgaacag cgtcatcatg atggtggtga aaatagtttt accgatatag
    10681 cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa ctggcgcgca
    10741 ggcggcggac cggcacggtg tcccaaaggt tgagaacaat cgccaaagcc agataaatca
    10801 ggttaacggt ccacatccac agcgtcgggt ccagcttacc tttaccgccg ttcgatttca
    10861 gggaggtctg gatcaggaag aaaagtagat acagcagcat ggctggcagc atcgacagta
    10921 cgcgtccctg acgtgggtta accacgctca gcggtacgac cataagtgcc atcataaaca
    10981 cggtgaatac caacgtgata cgccagttca gttctgcgcg agcacgatcg gtgtcagtgt
    11041 tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg gtcgagcgcc accgcctggt
    11101 gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg gaaatcacgt aacagtgcag
    11161 tgccttcgaa gcgcgttccc tggttgagag tgacgacctg ggagccgtcg cgcagctggg
    11221 ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc attacctttt ggtcgaattt
    11281 gcgcgaggaa cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac agcaccgaac
    11341 tgccattagt cgcttgctgg aattgccctt gcgccagcgc cgccatgcca gggttcgctt
    11401 tcgcttctgc taacacttca tcctgatgac gcgatgacca cggtcccgcc cacatcacgt
    11461 taatcgccgc gacgattgcc gtgaataccg caaggatcat tgccgctttc accagaaccg
    11521 ctttgctcag gccgcaggca tgcattaccg taatttcact ttcggtatac agtttgccca
    11581 gcgtcatcag cagcccgagg aacaggctta atggcaggat aagctgcgcc atttccggca
    11641 cgcccaaccc gagaagggag agcaccagat tcgccggaat atcgccgtca accgctgcgc
    11701 cgaggatcct cactaacttt tgacagaaga agatcaaaag caagatgaag agtatcgcca
    11761 gctggctttt gagcgtctcc cgcaccagat atcttatgat tatcacttta aatacgcccg
    11821 taaaaactcg tcttttgcag gattttagct tgtttcatgg cttaaacgtc atttattctc
    11881 ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac ggattcacct ctcagatttt
    11941 gttctgacgt gccaatgccg taataacgtt aagattaaca cgaagtcatc gcaacagcgg
    12001 acatgagtta cgaaagcttg caattctatc tgtagccacc gccgttgtct ttaagattca
    12061 ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca gcggagtgcc
    12121 tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga acagctcgat
    12181 aaaatcagcg atgggtacat cagcgccctg ctacgtcggg gcgaactgga aggaaaaccg
    12241 gggcagacat tgttgctgca ccatgttccg aatgtacttt ccgagcgaat tctccttatt
    12301 ggttgcggca aagaacgtga gctggatgag cgtcagtaca agcaggttat tcagaaaacc
    12361 attaatacgc tgaatgatac tggctcaatg gaagcggtct gctttctgac tgaactgcac
    12421 gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg tcgagacggc aaaagagacg
    12481 ctctacagtt tcgatcagct gaaaacgaac aagagcgaac cgcgtcgtcc gctgcgtaaa
    12541 atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc gatccagcac
    12601 ggtctggcga ttgccgccgg gattaaagca gcgaaagatc tcggcaatat gccgccgaat
    12661 atctgtaacg ccgcttacct cgcttcacaa gcgcgccagc tggctgacag ctacagcaag
    12721 aatgttatca cccgcgttat cggcgaacag cagatgaaag agctggggat gcattcctat
    12781 ctggcggtcg gtcagggttc gcaaaacgaa tcgctgatgt cggtgattga gtacaaaggc
    12841 aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta aaggtttaac cttcgactcc
    12901 ggcggtatct cgatcaagcc ttcagaaggc atggatgaga tgaagtacga tatgtgcggt
    12961 gcggcagcgg tttacggcgt gatgcgtatg gtcgcggagc tacaactgcc gattaacgtt
    13021 atcggcgtgt tggcaggctg cgaaaacatg cctggcgggc gtgcctatcg tccgggcgat
    13081 gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga ataccgacgc tgaaggccgc
    13141 ctggtactgt gcgacgtgtt aacttacgtt gagcgttttg agccggaagc ggtgattgac
    13201 gtggcgacgc tgaccggtgc ctgtgtgatc gcgctgggtc accacattac tggtctgatg
    13261 gcgaaccata acccactggc ccatgaactg attgccgcgt ctgaacaatc cggtgaccgc
    13321 gcatggcgct taccgctggg tgacgagtac caggaacagc tggagtccaa ttttgccgat
    13381 atggcgaaca ttggcggtcg tcctggtggg gcgattaccg caggttgctt cctgtcacgc
    13441 tttacccgta agtacaactg ggcgcacctg gatatcgcgg gaaccgcctg gcgctctggt
    13501 aaagcgaaag gcgcaaccgg tcgtccggta gcgttgctgg cacagttcct gttaaaccgc
    13561 gctgggttta acggcgaaga gtaattgcgt caggcaaggc tgttattgcc ggatgcggcg
    13621 tgaacgcctt atccgaccta cacagcactg aactcgtagg cctgataaga cgcgcaagcg
    13681 tcgcatcagg caatgctgtt attgtcggat gcggcatgaa cgccttatcc gacctacaca
    13741 gcaccgaact cgtaggcctg ataagacgcg gcagcgtcgc atcaggcaat gctgttattg
    13801 tcggatgcgg cgtgagcgcc ttatccgacc tacacacagc actgaactcg taggcctgat
    13861 aagacacaac agcgtcgcat caggcgctgc ggtgtatacc tgatgcgtat ttaaatccac
    13921 cacaagaagc cccatttatg aaaaacgcga cgttctacct tctggacaat gacaccaccg
    13981 tcgatggctt aagcgccgtt gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca
    14041 gcggtaagcg cgtgctcatc gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag
    14101 ccctatgggc gcgtccggca gaaagctttg ttccgcataa tttagcggga gaaggaccgc
    14161 gcggcggtgc gccggtggag atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata
    14221 tattgattag tctgcgaaca agctttgcag attttgccac cgctttcaca gaagtggtag
    14281 acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat aaagcctacc
    14341 gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga catataaccc
    14401 acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact ttaagcctaa
    14461 tggcgatgaa agccaggaga gtttctgcat catgatcccg ccgccgaacg tcaccggcag
    14521 tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc gctatcagcg
    14581 catgcagggc aaaaacaccc tgtggcaggt cggtactgac cacgccggga tcgctaccca
    14641 gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg actacggccg
    14701 cgaagctttc atcgacaaaa tctgggaatg gaaagcggag tccggcggca ccattacccg
    14761 tcagatgcgc cgtctcggca actccgtcga ctgggagcgt gaacgcttca cgatggacga
    14821 aggcctgtcc aatgcggtga aagaagtttt cgttcgtcta tataaagaag acctgattta
    14881 ccgtggcaaa cgcctggtaa actgggaccc gaaactgcgc accgctatct ctgacctgga
    14941 agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc tggctgacgg
    15001 tgcgaaaacc gcagacggta aagattatct ggtggtcgcg actacccgtc cggaaaccct
    15061 gctgggcgat actggcgtag ccgttaaccc ggaagatccg cgttacaaag atctgattgg
    15121 caaatatgtc attctgccgc tggttaaccg tcgtattccg atcgttggcg acgaacacgc
    15181 cgacatggaa aaaggcaccg gctgcgtgaa aatcactccg gcgcacgact ttaacgacta
    15241 tgaagtgggt aaacgtcacg cgctgccgat gatcaacatc ctgacctttg acggcgatat
    15301 ccgtgaaagc gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt attccagcga
    15361 aatcccggca gagttccaga aactcgagcg ttttgctgca cgtaaagcgg tcgttgctgc
    15421 ggttgacgca ctcggcctgc tggaagaaat taaaccgcac gacctgaccg ttccttacgg
    15481 cgaccgtggc ggcgtagtta tcgaaccaat gctgaccgac cagtggtacg tgcgtgccga
    15541 tgtactggcg aaaccggcgg ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa
    15601 gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt gtatctctcg
    15661 tcagctgtgg tggggtcacc gtatcccggc atggtacgac gaagcgggta acgtttatgt
    15721 tggccgcaac gaagatgaag tgcgtaaaga aaataacctc ggtgccgacg ttgctctacg
    15781 tcaggacgaa gacgttctcg acacctggtt ctcttctgcg ctgtggacct tctctaccct
    15841 tggctggccg gaaaacaccg acgccctgcg tcagttccac ccaaccagcg tgatggtatc
    15901 cggcttcgac atcatcttct tctggattgc ccgcatgatc atgatgacca tgcacttcat
    15961 caaagatgaa aatggcaaac cgcaggtgcc gttccacacc gtttacatga ctggtctgat
    16021 tcgtgatgac gaaggtcaga agatgtccaa atccaagggt aacgttattg acccgctgga
    16081 tatggttgac ggtatttcgc tgccggaact gctggagaaa cgtaccggca atatgatgca
    16141 gccgcagctg gcggacaaaa tccgtaagcg caccgagaag cagttcccga acggtattga
    16201 gccgcacggt actgacgcgc tgcgtttcac cctggcggca ctggcttcta ccggtcgtga
    16261 catcaactgg gatatgaagc gcctggaagg ttaccgtaac ttctgtaaca agctgtggaa
    16321 cgccagccgc tttgtgctga tgaacacaga aggtcaggat tgcggcttta acggcggtga
    16381 aatgacgctg tcgctggcgg atcgctggat tctggcggag ttcaaccaga ccatcaaagc
    16441 gtaccgcgaa gcgctggaca gcttccgctt cgatatcgcc gcaggcattc tgtatgagtt
    16501 cacctggaac cagttctgcg actggtatct ggagctgacc aagccggtaa tgaacggtgg
    16561 aacagacgcc gaactgcgcg gtactcgcca tacgctggtg actgtactgg aaggtctgct
    16621 gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc gtgtgaaagt
    16681 actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt atgatgcgtc
    16741 tcaggttgat gaagcggcgc tggccgacac cgagtggctg aaacaggcga tcgttgccgt
    16801 acgtaacatc cgtgcagaaa tgaatatcgc gccgggcaaa ccgctggagc tgctgctgcg
    16861 tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaaccct
    16921 ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac
    16981 gaagatcatc gacggcgcag agctgctgat cccgatggct ggcctcatca acaaagaaga
    17041 tgagctggcg cgtctggcga aagaagtggc gaagatcgaa ggcgaaatca gccgtatcga
    17101 gaacaagctg gcgaatgaag gctttgtcgc ccgcgcaccg gaagcggtca tcgcgaaaga
    17161 gcgtgagaag ctggaaggct atgcagaagc gaaagctaag ctgattgaac agcaggctgt
    17221 tatcgccgcg ctgtaatcaa aacacagtga tgaaaacgaa ggccggagca tgctccggcc
    17281 ttttttatct cttacagaaa tggtaaagaa ggcataattc cccgtgaaat actggctaag
    17341 aagcctttat ctggttgttc ttctttattg attaaaggaa ggtcatccaa atcgcccagc
    17401 aaatacgtat tttttgcctg ccagtcaatc atccatattt tcagcagtgg ataagccaga
    17461 ccgcccgtaa tcccggatat caccaccaac gcacacatgc gataaagcat accgtggtat
    17521 gttaaagttg agcgaaaacg aatacgccca tcattcagtg acaggttgct cataaaatga
    17581 tttcgcaacg acaccgttaa ataactggtg ctaacagcaa tcccaaaata atagattaac
    17641 tgcgcgatta tcattttacg ttgcatttcc ataaattgct gtaaattctc aatatcatca
    17701 tttgcataca cagaactatc atacgcattt aatatttgat cgaagataat ataaccagca
    17761 acagcgagaa aaggtaataa agctaaaaat gcgaggatgg catattttat acaatacgca
    17821 gtatctaatt tcaccttgaa acgatgtata ccgaaactgg tattgctcca gagaaaactc
    17881 attaccaggc tatataaagt accattaaaa ataccaatgg aaacaatacc aactattgcc
    17941 atcagaacca cagatataat aacactactt gaactattgg catgtagcat ctttgtcgag
    18001 ataaagaaaa cagtccccat cccaatggcc attaaaatcg gcaagaaaaa ggttacccac
    18061 cagaaccctt tcatagagca attaaaacta aatcttacgc cgttgagact ggtcatcaag
    18121 gcctgataac gcagtccttt tgctgccata aatataagca aaaccaacag tgacaaagtc
    18181 aaaacacagc caacaagcgg catatctgct gacactgtca ttaagattgc gaaatagaaa
    18241 aaaacaaaaa caagacaact aaaaaaaaca ttcccaccag taattccata agaaaatcgt
    18301 tgtccgttaa cttccatatt agcatagaga taacgcctac atttcattaa tgcccatggt
    18361 aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat aagaaaatat
    18421 ctctcacctg taccatgaaa aacaaacgaa t
//
LOCUS       DAZFRE010000096        18263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFRE010000096 DAZFRE010000000
VERSION     DAZFRE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.096"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            244..1023
                     /locus_tag="ACHKUI_003889"
     CDS             244..1023
                     /locus_tag="ACHKUI_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6599989.1"
                     /translation="MEHKPTIRVLEILEALAAENQGVSLTHLSEKTGILKGTIFPILK
                     TLVEQRYISYDANTQLYNLGISCSILSRAFLDRSYWLKMVYKEMNNIVKECNEVCQMG
                     ILDGADVLYINKVQAAQTVQLVSHIGTRLPAIYSALGKAIICEYSDQQIRQLYPDGFV
                     PMTSYSVTTLEQLRQQLEDARVNGYAFDNREINEETVCYAVALKQRNKTLAAISISIP
                     VFRATKEKIADVTRILLNARSRIEQELNTLQDINIGIDSHH"
     gene            complement(1100..1831)
                     /gene="rlmB"
                     /locus_tag="ACHKUI_003890"
     CDS             complement(1100..1831)
                     /gene="rlmB"
                     /locus_tag="ACHKUI_003890"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6599990.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(2011..4452)
                     /gene="rnr"
                     /locus_tag="ACHKUI_003891"
     CDS             complement(2011..4452)
                     /gene="rnr"
                     /locus_tag="ACHKUI_003891"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6599991.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(4491..4916)
                     /gene="nsrR"
                     /locus_tag="ACHKUI_003892"
     CDS             complement(4491..4916)
                     /gene="nsrR"
                     /locus_tag="ACHKUI_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6599992.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(5121..6419)
                     /gene="purA"
                     /locus_tag="ACHKUI_003893"
     CDS             complement(5121..6419)
                     /gene="purA"
                     /locus_tag="ACHKUI_003893"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6599993.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(6523..6720)
                     /gene="yjeT"
                     /locus_tag="ACHKUI_003894"
     CDS             complement(6523..6720)
                     /gene="yjeT"
                     /locus_tag="ACHKUI_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6599994.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6802..7806)
                     /gene="hflC"
                     /locus_tag="ACHKUI_003895"
     CDS             complement(6802..7806)
                     /gene="hflC"
                     /locus_tag="ACHKUI_003895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6599995.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7809..9068)
                     /gene="hflK"
                     /locus_tag="ACHKUI_003896"
     CDS             complement(7809..9068)
                     /gene="hflK"
                     /locus_tag="ACHKUI_003896"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6599996.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(9154..10434)
                     /gene="hflX"
                     /locus_tag="ACHKUI_003897"
     CDS             complement(9154..10434)
                     /gene="hflX"
                     /locus_tag="ACHKUI_003897"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6599997.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(10510..10818)
                     /gene="hfq"
                     /locus_tag="ACHKUI_003898"
     CDS             complement(10510..10818)
                     /gene="hfq"
                     /locus_tag="ACHKUI_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6599998.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10904..11854)
                     /gene="miaA"
                     /locus_tag="ACHKUI_003899"
     CDS             complement(10904..11854)
                     /gene="miaA"
                     /locus_tag="ACHKUI_003899"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6599999.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11847..13694)
                     /gene="mutL"
                     /locus_tag="ACHKUI_003900"
     CDS             complement(11847..13694)
                     /gene="mutL"
                     /locus_tag="ACHKUI_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6600000.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTISLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(13704..15035)
                     /gene="amiB"
                     /locus_tag="ACHKUI_003901"
     CDS             complement(13704..15035)
                     /gene="amiB"
                     /locus_tag="ACHKUI_003901"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6600001.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPVVAKRVETPAVVAPRASEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(15054..15515)
                     /gene="tsaE"
                     /locus_tag="ACHKUI_003902"
     CDS             complement(15054..15515)
                     /gene="tsaE"
                     /locus_tag="ACHKUI_003902"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6600002.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYSLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSSGELLLARLAG"
     gene            complement(15487..17034)
                     /gene="nnr"
                     /locus_tag="ACHKUI_003903"
     CDS             complement(15487..17034)
                     /gene="nnr"
                     /locus_tag="ACHKUI_003903"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6600003.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTHSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            17033..18172
                     /gene="queG"
                     /locus_tag="ACHKUI_003904"
     CDS             17033..18172
                     /gene="queG"
                     /locus_tag="ACHKUI_003904"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6600004.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEQFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(18155..18208)
                     /locus_tag="ACHKUI_003905"
     CDS             complement(18155..18208)
                     /locus_tag="ACHKUI_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600005.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         4225 a         5150 c         4540 g         4348 t
ORIGIN      
        1 tgagtcaaat ttagccaatc acagaagtga tacagctaag ctattgatat atttggagta
       61 attatgtcag agacctgcat aaaaaacaat cgcagttctt tttttatccg ttttatgacc
      121 agtatcacgc taaaaaagga ctgatgggct caacagcagt caaggaacgc gagggggtta
      181 atttacttga attataattc tccctagaat ggtgcattac tcatggtaag gaaaatagtc
      241 attatggaac ataagcctac catccgggtg ttagagatac tggaagcact ggcagcggag
      301 aatcagggtg tttcattaac tcacctttcg gaaaaaacag gcatcctgaa aggaacgatt
      361 tttcccatcc tcaaaacgct ggttgaacag cggtacatca gttatgacgc taatacccag
      421 ctttataatc tggggatctc ctgctcgatt ctgtcccgtg ctttcctcga cagatcgtac
      481 tggttaaaaa tggtttacaa agagatgaac aacattgtca aagagtgcaa tgaagtttgt
      541 cagatgggga tcctggacgg tgcagatgtt ttatatatca ataaggtgca ggcggcacaa
      601 accgtacaac tggtttcaca tattgggact cgtctgcctg ctatctattc tgcgctggga
      661 aaagccatta tctgtgaata cagtgatcaa caaattcggc aactctaccc tgacggtttt
      721 gttcccatga catcgtatag cgttacgacc ctggaacaat tgcgccaaca acttgaggat
      781 gccagagtaa atggctatgc atttgataac agggaaatca atgaggagac ggtatgttat
      841 gctgttgcct taaaacagcg aaataaaacg cttgccgcca tcagcatttc aatacccgta
      901 tttcgtgcga caaaagaaaa aattgccgat gtcacgcgta tcctgctgaa tgcacgttcg
      961 cgtattgaac aggaactcaa caccttacag gatatcaata tcggtattga ttcccaccat
     1021 taagggtatt ttccattcga agcccgatac gtaaaaatta tcgggctaac aaaatatcca
     1081 ttgaatggat agcgatgaac tagctgcgct ggcgcaccgc ttcaaataag caaattccgg
     1141 tcgcaaccga aacgttcagg gaagaaacgc ttccagccat cgggatgctg atcaactcat
     1201 cgcaatgttc acgagtcagg cgacgcatac cttcaccttc cgcacccatc accagcgcca
     1261 ggcgtccggt cattttgctc tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga
     1321 tccagatatt ctcttcctgc aacatacgca tggtgcgcgc aaggttagtc acccgaatca
     1381 gcggaacgct ttctgccgcg ccgcaggcta cttttttcgc cgtagcgttg agctgtgcgg
     1441 agcgatcttt cggcacaatc accgcgtgaa cgccagcagc gtccgcgcta cgcaggcacg
     1501 cgccgaggtt gtgcggatcg gttacaccgt cgaggatcag caggaacggt tgatcgagcg
     1561 aagcgatcaa atccggcaga tcgttttcct gatactgacg tcctggcttc acacgggcga
     1621 taatgccctg atgcacggca ccgtcgcttt tctcatcgag atactgacgg tttgccaact
     1681 ggataaccac gccctgggac tcaagggcgt gaatcagcgg taacagacgt ttatcttcac
     1741 ggccttttaa aataaagact tcctgaaaac gttcaggggc gcgctccagc agggcctgca
     1801 ctgcgtggat gccgtaaatc atttcgctca ttaatgtact cgttgtttac gttttgcctg
     1861 atgcactacg tttatccggc ctacatgatc tctgcaatat attgaatttg cgtgattatg
     1921 taggccggat aaggcgttca cgccgcatcc ggcatgaaca aagcgcattt tgtcagcaat
     1981 ctaactctct tcttttaaag agggtattga tcactctgcc acttttttct tcgccgcacg
     2041 cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc gatggctttt tcgcttttct
     2101 cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca ccgcggaagg cgctgtctgg
     2161 ctcaaagttt acctttttac cgacctgacg acgcttgccg ccttttttgc ctgcatcgcc
     2221 ttttttcgct ttctcgcgcg ccgttttacc gacattgcgc ggtgcgcgtt cgctggagat
     2281 caggctaaag tcgattttgc gctcgtccat attaaccgct tcgacgcgaa cttccacgcg
     2341 atcgcccagg cgataagtct ggccgctgga ttcacccatc agccgttgcc ctacctggtc
     2401 aaagcgatag tagtcattgt ccagcgaaga gacatggacc agaccatcaa tgaacaagtc
     2461 gtccagacgg acgaagaagc caaagccagt gacgctggaa attacgcctt taaagacgtt
     2521 acctacctgg tcgagcatga agtcacactt cagccagtca gcgacatcgc gcgttgcttc
     2581 gtcggcacga cgttccgcca tcgaacagtg ctgacccaat tgcagcatct cttccatcga
     2641 ataatggtag ccgccggttt cagtggtgtt gccctgatgc ccctgctctt tcgccagcag
     2701 atatttaatg gcgcggtgca gcgtcaggtc tggataacga cgaatcggcg aagtaaagtg
     2761 cgcataggac tgcaatgcca ggccaaagtg accacggttt tctggatcgt aaatcgcctg
     2821 tttcatcgag cgcagcagca tggtttgcag catttctgca tcaggacgat cggcaaccga
     2881 ctccagcagc tccgcgtagt cacgcggttc cggcttatta ccgcccggca gttccagccc
     2941 cagctccgcc agcactgaac ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat
     3001 acggaacagt gccggttctt tggctttctc aacaaaacgc gccgccgaga tattcgccag
     3061 aatcatgcac tcttcaatca acttgtgtgc gtcgttacgc tgagtctgtt cgatacgttc
     3121 aatacggcgt tcagcgttga aaatgaactt cgcttcttcg ctctcaaatg aaatcccgcc
     3181 gcgttcttca cgggctttat ccagcacttt atagaggtta tgcaactctt cgagatgctt
     3241 aaccagtggg gcgtactgct cgcgcagatc ctgatcgccc tgcagaatat gccagacttt
     3301 ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg tagaatttgt agccagtcag
     3361 gcggcctttc gacgaaaccg tcatctcgca caccatacac aggcggtcta cctgcgggtt
     3421 gagcgaacac aggccgttag agagcacttc cggcagcatc gggataacct gcgaagggaa
     3481 gtacaccgac gtgccacggt tacgcgcttc tctgtccagc ggcgttgacg gacgcacata
     3541 gtagctgacg tcggcaatcg cgacccataa acgccagccg ccgccgcgtt ttttctcgca
     3601 gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca atggtgacca gcggtaaatc
     3661 gcgcaaatcg acacggcccg cttttgcttc ttccggcact tcttctttca gcccggcaac
     3721 ctgttgctca acagcctgcg gccagatata cggaatttca tgggtacgca gagcgatatc
     3781 aaccgccatg ccggtgccca tattatcgcc aaggacttcg acgattttac ccaccgcttt
     3841 ggtgcggcga gtcggacgct gagtcagttc gactacgacc acaaagccca tccgcgcgcc
     3901 catgatctga tcgggcggga ttaagatatc gaagctcaga cggctatcgt caggaaccac
     3961 aaagccgact cccgcttcgg taaagtagcg accaacaatc tggctggttt ttggcaccag
     4021 tacgcggaca atacgcgctt cacgacgacc tttacggtca gcgcccagcg gctgagccag
     4081 cacctgatcg ccatgaatgc aggttttcat ctgctcgctg gagagataca aatcatcttt
     4141 acgcccttca acccgcagaa agccgtagcc atcacggtgg ccaataacgg tacctttcac
     4201 caggtcgagg cgttccggca gcgcatagca ctgacgacga gtgaagacca gttgaccatc
     4261 gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg
     4321 cagttctacc gccagctcat cacggctggc cggtttttca cgtttggtta aatgttcgag
     4381 gataaattcc cgactaggga tgggattcgc gtatttttca gcttcgcgtt cctggaaagg
     4441 atcttgtgac atctcggttc ctccgttgtc atctctgatg aagattttcg tcactccacc
     4501 agcaataatt tataaagcgg ttgattctct tcaaccaaat cggcaagcgt gtagttatcc
     4561 agttccgtaa gaaaactttg cacggcctta gaaagcgcct gtttcaaccg acaggcaggt
     4621 gtaatgtggc aaaactcact gctgcaattc accagcgata agggctccag ctcgcgcacc
     4681 acatcaccaa tacgtatcgc actcgccgat ttacccaggc gaatgccgcc attttttcca
     4741 cgaacagcag tcacgtagcc ggcacgacta agttgattga ttattttgac catatgatta
     4801 cgggagacgc cgtagacgtc agtcacttca gaaatactgg tcatccgccc ttctggcaat
     4861 gacgccatgt agatcagcgc acgtaatccg taatcagtga aactcgttaa ctgcacatca
     4921 acctcaaaag ggaaatcggg aaaaaataca tttatattga tgataaacca gccacaagct
     4981 gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg
     5041 atagcgtaat ggcttaaccg acacagcaat gtatcacact gttagcccgg caggcaaaat
     5101 atctgccagg cgtaccagaa ttacgcgtcg aacggatcgc gcagaatcat ggtttcagta
     5161 cgatccggac cggtagagat gatatcgatc ggcacaccag tcagctcttc aatacgcttg
     5221 atgtagttca gcgccgcctg cggcagaccg ctacgatctt tcacgccgaa ggtggattca
     5281 gaccagcccg gcatggtttc gtaaatcggc tctacacctt tccagtcgtc agctgccagc
     5341 ggagtggtcg tcacttcacg accatccggc atacggtaag ccacacagag tttcacctct
     5401 ttcaggccat ccagaacgtc cagtttagtc aggcagaagc cagacaggga gttcagctgt
     5461 accgcacgac gaacggcaac ggtgtccagc cagccggtac gacgacgacg ccccgtagtt
     5521 gcgccgaatt cgttaccctg cttgcagagg aactcgccag tttcatcaaa cagttcggtc
     5581 gggaacggac ctgcacctac acgagttgag taagctttga ggatacccag aacataatca
     5641 acataacgcg ggcccaggcc ggaaccggtc gccacgccac cagcagtggt gttggaagaa
     5701 gttacgtacg gataagtacc gtggtcgata tccagcagcg taccctgcgc accttcaaac
     5761 atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt cagaaacgtc aaccaccata
     5821 gaagtcagga tgtcggcaac agccatcgta tcatccagaa ctttctggta atcaaccgct
     5881 tcagctttgt agtagttaac caactggaag ttgtgatatt ccatcacttc tttcagtttt
     5941 tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca gaccgcgacg tgctacttta
     6001 tcttcataag caggcccgat accacgaccg gtggtgccga tcgctttcgc gccacgcgct
     6061 ttctcacgcg cgttatccag cgcaacgtga taatcaagga tcagcggaca tgcttcagac
     6121 agcagcagac gctcacgaac ggggatgcca cggtcttcca gttctttcat ctctttcatc
     6181 agcgcggccg gagacagcac aacaccgtta ccgatgatgc tggttacatt ctcgcggaga
     6241 atacctgatg gaataagatg gagaacggtt ttttcaccgt tgattacgag agtatggcct
     6301 gcgttgtgac cgccctggta gcgtacaaca tatttagccc gttcagtcag aagatcgacg
     6361 atcttacctt taccttcgtc accccattgg gtgcccagta cgacgacgtt gttacccatt
     6421 tttcaaaatc accgtttgct taaaaatgga ttctaccatc gctttttcag agttacagca
     6481 ctttttgcac tcaaaaatga cctcgatcag tctgtttttt gttcagccaa tcgttttcct
     6541 caacatgtag tagaccacaa cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat
     6601 attatcgggc aaattggtca tcgcagagat catcttcttc catgccttcg ggtaaagcat
     6661 cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc agccagattg tcgaattcat
     6721 tcattatcct tatagaaaaa gaaaaccacc gacatccctg aggatgcggt ggctttattg
     6781 acctgtaccg cagtcgttat attaacgcgt tgcggaagtc ggcgtcttca tgtagcggaa
     6841 gaaatcgcta tccgggctca tgaccatcac gtcctgattg ccagagaagc tgttctcata
     6901 agcacgcagg ctacggatga atgcgtagaa gtccggatct ttactgaatg catcagcaaa
     6961 cagtttggct gcttcggcat cgccttcacc acgcatgata cggccctgac gctcagcttc
     7021 tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc agtttttccg cttcttcctg
     7081 accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat
     7141 cgcttcagac acttcggtag gcaggttgat ctgcttaatt cgcacatcga caacttcaat
     7201 acccagcgcc gccatactgt tcgggttgat gaccggaact ttgcccttcg tctcagccgt
     7261 tacgcgctct gccgcttcgg caatggcgtt atctgccgcc ggggtagtaa cttcatcttc
     7321 tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg agggtcagac gaccacggga
     7381 atcggtgacg atgtctttca cgtccaggcg accaatttca gaacgcagac ggtcagagaa
     7441 cttacgtttc aacagcactt ccgcttgcga aatgtcgcca ccacccgttg ccaggtagta
     7501 acggctgaaa tcgctgatgc gccatttgat gtaagagtcg acgatcaggt ctttcttctc
     7561 tttggtcaca aagcggtcgg cctggttgtc catggtctga atacgtgcgt cgagcatttt
     7621 caccgtttca atgaacggta tcttgaaatg cagacccggc tcataaacca gaggtttgtt
     7681 gtcatcgtca cgcagtacct taccaaaacg cagcgtaata ccgcgctcac cttctttgac
     7741 gacaaagaca gacatgtaaa gcactaccag cacgatgatg ataatcgcga taactgactt
     7801 acgcatcgtt attccccctg acgctggtag tcgttacgct gcgcgttggc gcggcgttgg
     7861 tccataatat cgccctgact ggtggacgac gtgttgcttg ctccactggt tgtggaggaa
     7921 gaggctggcg gcagacgcag cagattgctg gcaccgttat cgctcttcgc cgcaggggcg
     7981 ttaccacctt tcagcatctg gtctaacggc agaaccatca ggttgccacc tttatcgtta
     8041 accagcactt tgcgggtgtt gcccaacact ttttccatcg tctcgatata cagacgctcg
     8101 cgagtaattt ccggcgcggc tttatattcc ggcagaagtt tagcaaagcg cgccacttca
     8161 ccctgggctt ccaggatggt ctgggccttg tacgcacgcg cctcttcgag gatacgttgc
     8221 gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg cttctgcttc acgaatgtat
     8281 tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc
     8341 ggacgagcag cctggaagtt gacgtccagc agagtgatgc ccatgtcata cggacgaatc
     8401 gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg tacgaccttc cgtcagaatg
     8461 cggtccatgg tgtatttacc gataaccccg cgcagggcgc tgtcggtagc ctgacgcaga
     8521 ctgtcatccg ggctggtcac gctatacaga tatttttccg gatcggtgac gcggtactgc
     8581 acgttcatct caacgcgcac tacgttctcg tcagacgtca gcatcacacc agaagctgcc
     8641 agttcacgca cggcttccac gttcaccggt ttgacttcgt caataaacgt cggtttccag
     8701 ttcagacccg gctcaaccag atggctgaat ttaccaaagc gtgttaccac gccgcgttcg
     8761 gcttctttaa tggtatagaa accactggcc gcccagataa tgacaatcgc tgccgctgcg
     8821 atggtaacga cgcgaccgcc aagctgcggg cgcgggcctt gcgatgaact gccaccgcca
     8881 gatccggtgc ctttaccgcc gcccagacca ccgagctttt tgctcagttt gcggaagata
     8941 tcatctaaat caggtggccc ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg
     9001 ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc cgttattacc gggctgattc
     9061 cacgccatgt ttgtgctcca tatttgttat gcggtgatcc ctgttgtctt tcgacgccag
     9121 aggatatgac tccacgcttc agacgctacg ccgttagatc aggtaatcga tcaacgccgg
     9181 ttcttgttta cagaggcgac gccagtcaac gatcggcata cgaacttgca gacttacgct
     9241 gccgtcctcc tccatccact ctttttctat tgcctgaagc tgataaaaac gacttctcag
     9301 gcgcccttcc tgcggcggca gacgcaatgt atgctgcgcc acctcgccag aaagacgctc
     9361 cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc tgcgcagaaa gccagacacg
     9421 gatcggtttg ttctcttcat ctctgtcaat acgcggttcg aaatcgtcca gcatatcgat
     9481 cttgttcatc accagcaggg ttgggatctc gtgagcatca atctcttcaa gaaccgtatt
     9541 caccgcttcg atgttttctt gtacacgcac atccgccgcg tcaatgacgt gcagcagcaa
     9601 tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgcc gccaccagat catgcggcag
     9661 gtggcgaata aaccctacgg tatctgccaa cacggtttca ccgacatccg caacgtcaat
     9721 acgccgcaac gtcgggtcga gggtggcaaa caactggtct gccgcgtaga cccgcgcttc
     9781 ggtgattcga ttgaaaagag tagatttacc ggcgttggta tatcccacca gcgaaacagt
     9841 aggaacgtcg gctttgatac gcgattgccg cccctgctca cgctgctttt caactctttc
     9901 caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg
     9961 ggtttcaccc ggaccacgca aaccaatccc gcctttctgt ctttcaaggt gggtccagcc
    10021 acgcaccagg cgcgtagcca gatggcgcaa ctgcgccaac tcaacctgca acttaccctc
    10081 atgggtacgc gcacgttggg cgaaaatatc taaaataagg ccggtgcggt cgatgacacg
    10141 acactcgcac aaacgctcca ggttacgctc ctgcgccggg ctcagggcat ggtcaaaaag
    10201 aacgaccgaa gctcccgtcg cttttacggc ttccgcaatt tcaactgctt taccttcacc
    10261 tacaaaatac tttgggtgcg gcgctttacg gctaccggta atcacctgca atgcttcgac
    10321 accggcggaa gagaccagag attcaaactc ctggaggtct tccatatctt tgtcttgcgt
    10381 aaaatagatg tgtaccagta ccgcctgctc accagcatca taacggtcaa acaagcgtat
    10441 aaccctctaa atagatcagc ggggaacgca ggatcgctgg ctccccgtgt aaaaaaacag
    10501 cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct
    10561 accatgatga tagttactgc tggtaccgcc accggcgttg ttactgtgat gagaaaccgg
    10621 gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt
    10681 caacaggatc acgaactgat caaaagactc gatttgtcct tgcagcttaa taccattcac
    10741 caaataaata gaaactggaa cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa
    10801 agattgcccc ttagccattc tctcttttcc ttatatgctt atttgtactt tgaacctttc
    10861 gattctgaaa aaattgcgca cgatacgtct caattgtaca cattcagcct gcgatagcac
    10921 caacaacctg taatacttcg tcacgcgcct gttctggttt ttcactgtca agccagtgaa
    10981 ccccttccca accacgcagc caggttatct gccgcttcgc caactgtctc gtggcgcaaa
    11041 cacctcgata aaccatttca tcgtatgaga tttcgccttc aaggtaagac cacatctggc
    11101 gataacccac gcaacgaatg gaaggcaaat ccgtatgcaa atctcctcgg gcaaaaagcg
    11161 cccggacttc tgcttcaaaa cctgaagcca acatctgatg aaaacgctgc tcaattcgtt
    11221 gatggagcag ttcacggctc gccggggcga tggcgaactg atgcacctga tacggtagag
    11281 cgtctcctga cgtttgcgtc agttccgtta aagttttacc cgaaatgaaa aaaacttcca
    11341 gtgcccggga aagcctttgt ggatcatttg gatgaatcct tgctgccgca accggatcga
    11401 tctcctgaag ttgacgatgc aatgactccc aaccttgctc tgccgcctgt tgctcaattc
    11461 tggcccgtac ttccgggtct gccgacggta gcggcgacaa cccttccagc aatgccttga
    11521 aatacaacat cgtaccgccc actaacagtg ggatccgccc cgccgcggtg atatcggcca
    11581 tttccgccag cgcatcgcgg cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa
    11641 tattcagcaa tcggtgcggc gcggcgagta actcttcagc gttcggcttc gccgtcccga
    11701 tatccatccc tttgtaaata agggcagaat caacgcttat caactctact ggtaaaattt
    11761 tacgcagctc aatggctaac gccgttttac cggaggccgt cggccccatc aaaaaaatcg
    11821 ccttaggcag gctcgcctta ctgatatcac tcatctttca gggcttttat cgccggatgt
    11881 aaatcaacag attgtaacag accacccggc ggcgttttca caagttgcgg acataaccgt
    11941 tccacgtccg ccagcagggt tatggcctgt gccattgacc actgcgcatg ttcgctcatc
    12001 agatttcgtg caatccactg cgcaatattg ccaggttcga atacggactg cttcgccagg
    12061 tagcctatca gttcaggaat caagatttgt aaattttgtt ggcgtaaggg taaaggcacg
    12121 gccctgatgg tcacatgctg cgcatcggac tggaaatcaa tacccagttc cgccagggca
    12181 gactgcgctt tttctaatgc cgatttttct tcgccagaaa cttttagccg caacggaata
    12241 agcagcggtt gggcgcaaac tggcacttca cccggcgtca attgtgcctg acgcagccaa
    12301 cgttctgcca ctggcaagga taaaagtgaa atgttgccat cgcgctcaag caacgcacag
    12361 tcggaatgga cgatagtcag tacccgacca aaactctgac tgttcgccgc aagtgcaggt
    12421 tcctgcggtt ctggcgcttt tggtttttgc atcggagcgg gcgtttgcaa aagctggcga
    12481 tacacttcac cttgctgttt ctggtaacca ggctgcgcat tcggccagga ggcagccgga
    12541 cgactgcctg aggcgggcgc aggagagtag cgcggagcta ccggctcacg aactgccggt
    12601 tcagcaaagt gattgcgccc agccgccacg cgattttccg gaatggcacg cggtgcaggt
    12661 tgtggttcat cgtccagcgg tagcggcgtt tccagttgct gttgtagcac gctcagcacg
    12721 ccctgataga taaagtcatg caccagacgc gactgatgga aacgcacttc gtgtttggca
    12781 gggtgcacgt tgacgtccac ctgatgcggg tcgatctcca gatacaacac aaatgccggt
    12841 tgctgatcgg cccccagttt gtcttcgcag gcctggcgga tcgcgtgatt gatcaggcga
    12901 tcgcgcatca tgcgaccgtt cacgtagcaa tactgaattt ctgccagtgc gggcgtagtg
    12961 tgatttggat cggccaccca gccgcgcagc gtgagatcgc cgtgttgcca ttcaatcgcc
    13021 agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta aacgccgttc tttttgcccg
    13081 ccttccggca ctgcacgata ctggcgtacg attttgccgt tatgcgacag actgatagtg
    13141 acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat cgatatggtt aaattcggtt
    13201 ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt agaacaggtc cagcacctcc
    13261 agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca cgtccatatc gcgcccttcg
    13321 gcataggcct gccaggcttc ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa
    13381 accgaactga tactcgccag cgcctcaccg cgaaagccca gactgataat ggcttcgaga
    13441 tcgtccagag aggcgatttt actggtggca tgacgcgcca gcgccagcgc cagctcgtct
    13501 tttttgatac cgcagccgtt atcacgaatg cgaataagtt tcgccccacc gcgttcgata
    13561 tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt ccaccagttc tttgactacc
    13621 gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt tcgccagttg tggcggtaag
    13681 acctgaattg gcatcaatcg tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta
    13741 ctggccgttt gtgccgttgc accctgcggc gcagattgca tcggatgcgc aaggaagtaa
    13801 ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc
    13861 aaacgttctt cgctgttgtt actgataaaa ccggtttcga ccagtactga cggaatatcc
    13921 ggcgaacgca gaacgccgag actcgcgtgt tctggtcggc gtttatgtat ttcgccaatg
    13981 cgttgcaact gactgatcat actggtcgct acatcatacc ctacccgctg ggaatgaccg
    14041 aactgtaaat ccagcaccgc ctggcttaaa taggggtcag actgactgtt cgccagcaca
    14101 tcacccgccc cgcccagcag ctccgactgt ttctcgtgct gctccagcca gctggccatt
    14161 tcgctgttgg cgcgacggtt agagagcacc catacggaag cgccagtcgc actgcggttc
    14221 ggtgctgcat cggcatgaat cgacacaagg aaattggcgt tttgcttacg tgccacatca
    14281 ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg ttaaaacgcc cttaaacatc
    14341 ggatcgttat tgagcaaagt acgcaattta cgcgcgatgg cgatggtgac atttttctcc
    14401 cgcgtaccac cggggccgat agcgccaggg tcctgaccgc cgtgtccggc atcaatagcg
    14461 atgataattt tatcgccagt gttagccgtc gcgcgggctg ccggacgcgt taccgtatta
    14521 ctgctgataa cacccgtagt gcggttactt tccgttttaa acggattgcg cgccggttcg
    14581 ctggcgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt tcgcaaccac tggcggaggc
    14641 ggtggcggcg catcggcgtt aatcgtaaag acaacagtgt agttgctgcc attctgccgc
    14701 ttcaccgctt cggttttacc gttttcggtg agatcgacca ccagccgtag cgtttgtgca
    14761 tctttaggcg ttccggagcg aatcgccttc accagattat taccgctgaa caacaacggc
    14821 agcccctgaa ttacccccgt ttgtttgata tcgagcgcca cggtgcgttt gctttgatgg
    14881 ctaaacgcat aatcaggatc gccaataaaa ctcaacgtta tccgtgcctg ttgattaccg
    14941 ttagaaacct gaatatcaga gagcgtcgcg gcacccaccg gcgcgcacag cagcagcaac
    15001 gtcgctacca accaatttct gatgcgatac atcatcccgc cacctttcaa aggttaaccg
    15061 gctaaacgcg ccagcaacaa ttcacccgaa gaggaaaccg cactcacgcg cgcctcacgg
    15121 ccttgtgcct gataatcaat gtgtatttcg acatccgggt caggaagaac acctgtacct
    15181 tgttgtggcc actccaccag gcagatggca tcgttggcaa aataatcgcg gatccccata
    15241 aactccagct cctcgggatc ggcaaggcgg tacaaatcaa agtgataaac cattaaattg
    15301 tcgagcgaat agggttcgac cagcgtataa gtggggcttt tgacattacc ctgatgacct
    15361 agagcctgta aaaagccccg gctaaaggtg gttttacctg cacctaaatc gccatacaga
    15421 tagattacgg ttgcgccatc gcaggctttc gctacccgct cgcccaggtc taatgttgcc
    15481 tgctcatcag ggagcggaat tactcgattc atcatggttt ttatcagtca cttccgggtt
    15541 aacaatacgc tgtagcgtgg aaaagagatc ggttgccagc atcccgcgcg ttccaaaacg
    15601 cgccgccagt acgtcagctg ccgcaccgtg cgcgacacag cccgcacagg ctgcatcata
    15661 agggctcatt ttttgcccaa gcaatgcgcc aataatacca gagagcacat cgcccatgcc
    15721 gccgctcgcc atgcctgcat ttccggcatc aataatgcct aaagcgtcag gatgggcggc
    15781 gaccacggtt ccggcacctt tcagcaccgc tacgccgcca taacgttgta ccagacgttg
    15841 ggcgcaatgt aagcggtcac tttcaatttc agcgacggaa cagcctaaca accgtgcggc
    15901 ctcgccagga tgcggcgtga tcacgcgatt gtgacgctta tcgggattga ttgccagcag
    15961 gttcaatgca tcggcatccc acaacatcgg tttgcgaaaa ttctcaactt tttgcagggc
    16021 ttttttcccc cactcttgct ggcccagacc gggaccaatc accaccacat cggcccattc
    16081 caggctttcg gtaagagagt ccatagtcag ttcatgcacc atcaattccg gtcgtgcagt
    16141 cagcagcggc gcaatgttct cactgtgggt cagtactcgg actaaaccag caccagcacg
    16201 cagcgccgct tcccccgtca tacgaatagc ccccgccgtg ccgtgatcgc caccaataat
    16261 caccagccgc ccgtgatcgc ctttatgcga agtcgggcga cgcggtatca gccagtgaga
    16321 aagttgttct gccgaaaacc gctgaatttt cgtctcctga cctgccagcc agctatccag
    16381 ccccagtgag tcaaaatgca gttgtccggt aacatcccgt gcttttccag tgagcaagcc
    16441 tggtttcagc gcaataaaag tgatggtgtg atcggcgttg attaccgcgc ctggcgtagc
    16501 gccggtttca gccagaaggc cggaagggat atcaaccgcc acaatcggcg cagggtgagt
    16561 attagcgtgg tcgattaact ggctaatgga ttcgcggggc gcttgctgca agccggtgcc
    16621 gagcagcgca tcaacaatca gatctaccga ttcgggccag acaatattcg aagcatggat
    16681 ctcgcctccc gcgtttaacc atgcttcgcg tgccagcgcg gcctcttccg gcaacggttt
    16741 gtcactttcc tgggccagca gcgtgacatc aataccgatc gctttggcca gccgcgcgac
    16801 cacgtagcca tcgccgccgt tattaccatg accgcacagc accagccagt ggcgggcgtc
    16861 aggataagcc gaacgacaca cctggaatgc ggcttcgcca gcgcgaagca tcagctcata
    16921 gagtgtgagc cccagcgcat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
    16981 ccagactgtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
    17041 gcccctcgat ctcaatcaat tagcgcaaaa tattaaacag tgggggctgg aactgggctt
    17101 tcagcaggta ggtattaccg ataccgatct cagcgcgtcc gagcccaaac tgcaagcatg
    17161 gctggacaaa caataccacg gcgaaatgga ctggatggcg cgtcacggta tgctgcgcgc
    17221 tcgccctcac gaactattgc ccggtacgct gcgtgtgatc agcgtgcgga tgaattacct
    17281 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
    17341 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
    17401 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
    17461 tcttgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
    17521 cctcaatcgt gaggccggct cgttcttctt tttaggcgaa ttgctggtcg atattccgct
    17581 acccgtggat caaccagtcg aggaaggatg cggtaaatgc gtggcctgta tgacgatttg
    17641 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
    17701 caccatcgaa ctggaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
    17761 ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac
    17821 agaagacgat ttcagcccgc gtaagccgct acacgctccg gaactcattg agttattcgc
    17881 ctggagcgaa gagcagtttt taaaagtcac ggaaggttcg gcgattcgcc gtataggtca
    17941 cctgcgttgg ctgcgtaata tcgccgtagc attaggcaat gccccctggg atgaaacgat
    18001 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt cttgatgagc acatagcgtg
    18061 ggcgattgcg cagcaaatcg agagacgaaa tgcgtgcgtg gtcgaagtgc aattaccgaa
    18121 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
    18181 cagcttgtga ataaaaataa aaacgcattg caattcaagc cgaaaaaatc gtcaagtgat
    18241 cgatacgaca aaacgaaatc tta
//
LOCUS       DAZFRE010000097        17949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFRE010000097 DAZFRE010000000
VERSION     DAZFRE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.097"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            108..1583
                     /gene="cdgI"
                     /locus_tag="ACHKUI_003906"
     CDS             108..1583
                     /gene="cdgI"
                     /locus_tag="ACHKUI_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6600006.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1764..3254
                     /gene="dgcJ"
                     /locus_tag="ACHKUI_003907"
     CDS             1764..3254
                     /gene="dgcJ"
                     /locus_tag="ACHKUI_003907"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6600007.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3297..3800
                     /gene="yeaK"
                     /locus_tag="ACHKUI_003908"
     CDS             3297..3800
                     /gene="yeaK"
                     /locus_tag="ACHKUI_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6600008.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3801..3905)
                     /gene="yoaI"
                     /locus_tag="ACHKUI_003909"
     CDS             complement(3801..3905)
                     /gene="yoaI"
                     /locus_tag="ACHKUI_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6600009.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            4075..4521
                     /gene="yeaL"
                     /locus_tag="ACHKUI_003910"
     CDS             4075..4521
                     /gene="yeaL"
                     /locus_tag="ACHKUI_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6600010.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4478..5299)
                     /gene="nimR"
                     /locus_tag="ACHKUI_003911"
     CDS             complement(4478..5299)
                     /gene="nimR"
                     /locus_tag="ACHKUI_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6600011.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5396..6577
                     /gene="nimT"
                     /locus_tag="ACHKUI_003912"
     CDS             5396..6577
                     /gene="nimT"
                     /locus_tag="ACHKUI_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6600012.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAIIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLLGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6632..6979
                     /gene="yeaO"
                     /locus_tag="ACHKUI_003913"
     CDS             6632..6979
                     /gene="yeaO"
                     /locus_tag="ACHKUI_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6600013.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(7001..7255)
                     /gene="yoaF"
                     /locus_tag="ACHKUI_003914"
     CDS             complement(7001..7255)
                     /gene="yoaF"
                     /locus_tag="ACHKUI_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6600014.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7438..8463
                     /gene="dgcP"
                     /locus_tag="ACHKUI_003915"
     CDS             7438..8463
                     /gene="dgcP"
                     /locus_tag="ACHKUI_003915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO6600015.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            8496..8594
                     /gene="yoaK"
                     /locus_tag="ACHKUI_003916"
     CDS             8496..8594
                     /gene="yoaK"
                     /locus_tag="ACHKUI_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6600016.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8597..8671
                     /gene="yoaJ"
                     /locus_tag="ACHKUI_003917"
     CDS             8597..8671
                     /gene="yoaJ"
                     /locus_tag="ACHKUI_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6600017.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8730..8978)
                     /gene="yeaQ"
                     /locus_tag="ACHKUI_003918"
     CDS             complement(8730..8978)
                     /gene="yeaQ"
                     /locus_tag="ACHKUI_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6600018.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9126..9308)
                     /gene="yoaG"
                     /locus_tag="ACHKUI_003919"
     CDS             complement(9126..9308)
                     /gene="yoaG"
                     /locus_tag="ACHKUI_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6600019.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9312..9671)
                     /gene="yeaR"
                     /locus_tag="ACHKUI_003920"
     CDS             complement(9312..9671)
                     /gene="yeaR"
                     /locus_tag="ACHKUI_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6600020.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9844..10482)
                     /gene="leuE"
                     /locus_tag="ACHKUI_003921"
     CDS             complement(9844..10482)
                     /gene="leuE"
                     /locus_tag="ACHKUI_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6600021.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10609..10776)
                     /locus_tag="ACHKUI_003922"
                     /pseudo
     CDS             complement(10609..10776)
                     /locus_tag="ACHKUI_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10777..10932
                     /locus_tag="ACHKUI_003923"
                     /pseudo
     CDS             10777..10932
                     /locus_tag="ACHKUI_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(11025..12140)
                     /gene="rnd"
                     /locus_tag="ACHKUI_003924"
     CDS             complement(11025..12140)
                     /gene="rnd"
                     /locus_tag="ACHKUI_003924"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6600022.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(12222..13907)
                     /gene="fadD"
                     /locus_tag="ACHKUI_003925"
     CDS             complement(12222..13907)
                     /gene="fadD"
                     /locus_tag="ACHKUI_003925"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6600023.1"
                     /translation="MKKVWLNRYPTDVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(14112..14693)
                     /gene="yeaY"
                     /locus_tag="ACHKUI_003926"
     CDS             complement(14112..14693)
                     /gene="yeaY"
                     /locus_tag="ACHKUI_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6600024.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14733..15428)
                     /gene="tsaB"
                     /locus_tag="ACHKUI_003927"
     CDS             complement(14733..15428)
                     /gene="tsaB"
                     /locus_tag="ACHKUI_003927"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6600025.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQEA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(15486..17396)
                     /gene="yoaA"
                     /locus_tag="ACHKUI_003928"
     CDS             complement(15486..17396)
                     /gene="yoaA"
                     /locus_tag="ACHKUI_003928"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6600026.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            17528..17872
                     /gene="yoaB"
                     /locus_tag="ACHKUI_003929"
     CDS             17528..17872
                     /gene="yoaB"
                     /locus_tag="ACHKUI_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6600027.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4470 a         4536 c         4260 g         4683 t
ORIGIN      
        1 ctcactgtaa attatctcat ttattacata cattagctta caatcgcttt aaatatgaca
       61 gcataacctt tacacaattt agttccagaa aacaatcatt cggaaaaatg attcggtcaa
      121 cacgtatttc cctggggtta ttctttaatt attttttatc gttaacgaaa attgatcttg
      181 gtcaaaacta tatatctctg ccatcaataa aatccagcac tcacattgct ctccttttta
      241 tggtttctat gggtacacaa aaattaaaag ctcaaggctt ttttattttc agcttattgc
      301 tgacgttaat tttattttgc attactacct tatataacga aaatacaaat gtaaaactca
      361 tcccacagat gaattacctg atggttgttg tggctttgtt tttccttaac gccgtcattt
      421 tccttttcat gttaatgaag tatttcacta acaaacaaat tttaccaaca ctcattttaa
      481 gccttgcatt tttaagtggc cttatctatt tagttgaaac cattgtaatt atccataaac
      541 caatcaacgg cagtacactg atccagacaa agtcgaatga tgtttccatt ttctatattt
      601 tccgccaact cagtttcatt tgtttaacct cgctggcgct cttttgttat gggaaagaca
      661 acatccttga caacaataag aagaaaactg gcattctgtt gctggcgctg attccttttt
      721 tagtttttcc ccttctggca cacaatctga gcagttataa cgctgactat tctttgtatg
      781 tcgtcgatta ctgtccggac aaccatactg cgacctgggg aatcaactat acaaaaatat
      841 tggtttgttt gtgggcattt ttactgttct ttattatcat gcgtacacga ttagccagcg
      901 aactatggcc gttaatagca ttattatgtc tggcatcgct atgctgcaac ttacttctac
      961 tgactctgga tgagtataat tacaccatct ggtatatcag tcgcgggatt gaagtttcca
     1021 gtaaactgtt tgttgtgtct tttctgattt ataacatttt tcaggaactg caactctcca
     1081 gcaaactggc agttcatgat gtgctgacca atatttataa tcggcgctac tttttcaaca
     1141 gcgtagagtc attattgtcg cgacctgttg ttaaggactt ctgtgtcatg ctggttgata
     1201 ttaatcagtt caaacgcatc aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg
     1261 tttcaattgt cgatattatc cagcaaagca tccgccccga tgatatttta gcgcgactgg
     1321 agggtgaggt atttggcttg ctatttaccg aactcaatag tgcccaggca aaaatcattg
     1381 cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt tagtaacaga tatgatgttc
     1441 ctgaacaaat gaccatcagt attggcacgg ttttttcaac gggtgacacg cataatatct
     1501 cgcttgtcat gacggaagca gataaagcct tacgcgaagc gaaaagcgag gggggcaaca
     1561 aagtgattat tcatcatatt taagtgcaaa aatattcaga gccatgcttt ttgcgtggct
     1621 tttgcataca atttattact attaccccta aatttcccct ccccagcggc gcagagatga
     1681 gtataattag cgcccctgtg ccaggccgca atcgaacttt atctggtttt ctcgtttcac
     1741 taaccgaagg agtgccattt atcatgaaat tgcaccatag aatgctccgg cattttatcg
     1801 ccgcaagtgt cattgtgctg acatcttcct tccttatttt tgaacttgtc gccagcgaca
     1861 gagcaatgag tgcctatctg cgctatatcg tgcagagagc agactcctcc tttctttatg
     1921 ataagtatca gaatcagagt attgccgcgc atgtgatgcg cgctctcgct gctgagcagt
     1981 cggaagtgcc gccagaacag cggcgcgcca tctgcgaggc ttttgagtct gccaataata
     2041 cccatggctt aaacctgact gcccataaat acccgggctt acgcggcaca ttacaaaccg
     2101 catccactga ctgcgacaca attgtggaag ctgcagcact attacccgct tttgatcagg
     2161 cagtggaagg caaccgccac caggatgatt acggttcagg tcttgggatg gccgaagaga
     2221 aatttcacta ttatctcgat ctcaatgacc gctatgtcta tttttatgag ccggttaatg
     2281 ttgaatactt tgcgatgaat aactggtcct tcctgcagtc aggaagtatt ggtatcgatc
     2341 gcaaagatat tgaaaaagta tttaccgggc gtaccgtatt atcgagcatt taccaggatc
     2401 agcgtactaa acagaacgtg atgagtttgc tgacgccggt atatgtcgca gggcagctaa
     2461 aagggattgt gctgctggat attaacaaaa acaatctgcg gaatatcttt tacactcatg
     2521 accgccctct cctctggcgt tttctcaatg tcacgctaac cgatacagat tcggggcgcg
     2581 acattatcat caaccagagc gaagataatc tgttccagta tgtcagttac gtccatgact
     2641 taccgggcgg cattcgtgtc tcgttatcca ttgatattct ttactttatc acgtcttcgt
     2701 ggaaaagcgt tctgttctgg attttgacgg cgttaatttt gctgaatatg gtgcggatgc
     2761 acttccgctt ataccaaaat gtgtcgcgag aaaatattag tgatgcgatg actggactct
     2821 ataatcgcaa aattttaacc cctgaactgg agcagcggtt gcagaaactg gtgcaatccg
     2881 gttcttcggt gatgtttatt gctattgaca tggacaagtt aaagcaaata aatgacaccc
     2941 tcggtcatca ggagggggat ttagcgatta cgttattagc tcaggcgatt aaacagtcga
     3001 ttcgtaaaag tgattatgcc atccgactcg gtggcgatga attctgcatc attcttgtcg
     3061 attcgacgcc gcaaattgca gcacaactgc ctgaacgtat cgaaaaacgt ctgcaacata
     3121 tcgcgccgca gaaagagatc ggcttctctt ccggtattta cgcgatgaaa gaaaacgata
     3181 cgttacatga tgcgtataaa gcttccgatg agcgtttata tgtcaataag cagaacaaaa
     3241 acagccgttc atgataatct tctgtggttg tttgcttgta atctcaggag catgaaatga
     3301 ctgaaatggc taaaggaagc gtgacgcatc agcggttaat cgctttatta tcacaagaag
     3361 gtgctaactt ccgcgtcgtt acccatgaag cggtaggtaa atgtgaagcg gtgtcagaaa
     3421 ttcgtggcac cgctttaggc cagggggcta aggcactggt atgcaaagtc aaaggcaatg
     3481 gcgtaaatca acatgttctg gcgatcctcg ctgccgatca acaggccgat ctgagccaac
     3541 tcgccagcca tataggcgga ttacgcgctt cgttggccag cccggcggaa gtcgatgaac
     3601 tcacaggctg tgtcttcggc gcgatccccc ctttcagctt ccatccaaaa ctcaaactgg
     3661 ttgccgaccc cctactcttc gaacgatttg atgaaatcgc cttcaatgca ggcatgctgg
     3721 ataaatccgt tattttgaaa accgcggatt atttgcgtat tgcgcaacca gaactggtca
     3781 atttccgccg cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc
     3841 acaactgcaa tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg
     3901 ttcatagtat gcccccggga gatagctgtt ttcacgttat tgttaacgta cacaaatggc
     3961 agtttgatga cagttcgtct gattttttat gcaaaaaacg taaagatttt ctacttcctt
     4021 cctgcagcaa gcgtaaagta agcaggctca ttattttttg gcaaggaaac cacgatgttt
     4081 gatgtcactt tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc
     4141 actgtcgccg tttcaattct ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt
     4201 ttcccgtgga ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt
     4261 atggccccca tcgccagcgg gacgctaccg ccctcaaccc tgattcactc gtttctgaac
     4321 tggaaatcgc tggtggcaat tgcagtcggc gttattgtct cctggctggg ggggcgcggc
     4381 gtgacgttaa tgggcagcca gccgcaactg gtcgccgggc tgctggtggg cactgtttta
     4441 ggcgtggcgc tgtttcgcgg cgtaccggtt ggcccgctta ttgccgcggg tctggtttcg
     4501 ctgattgtgg ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc
     4561 ctttcttaaa catagtaata aaggccgttg ttgaatcata ccccagcgta tgagcaactt
     4621 tctgtacagt atcgcctttc actaatccct gaagcgccat aatcagctgt aactgctgac
     4681 gccactggcg aaaactcaac cccgtctctt tcactatcag ccgggcaaga ttgcgttcac
     4741 tcatcgcaaa aaagccagcc cattgcccca atgagcccca ctcgacgggc tctttcgcca
     4801 tcatctccac catcgtgcgg attttaggat gagaagaaac gggcaggtgc agctgttgtt
     4861 gtggctgctg agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag
     4921 cacgctgagt cgtcgtcctg tttgccaggg ttaaaattaa ttcccggcat aagggcgata
     4981 ttttcaatgt gcaacatgtt gtcggcattg tcactgcgct gggctcgata aacagaaaac
     5041 agagttcagc gttagctgtc acttgattac tatgtttcac cccgcccggt atccaaaccg
     5101 catattgtgg cggcaccatc cacaaagcat tttccaccgt acaggtaatt gcaccatgta
     5161 gtgccagtat caactgccct ttgcggtgct gatgtacagg actggttaac tcatccgttc
     5221 cggcgtgaat acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga
     5281 gattcaacct gtgcatcatt ttgtccgaat ttagcgatat tttgtcattt tagcttgatt
     5341 caacataaca ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac
     5401 ctgttcaact tcattaagcg gcaaaaacag gattgtcctt atcgctggca ttctgatgat
     5461 tgccacaaca ttacgcgtca cctttaccgg cgcagcaccg ttactggata cgattcgttc
     5521 cgcttactcg ctaacgacgg cgcaaaccgg cttattgacc accctgccat tattagcctt
     5581 tgcgctaatc tcaccgctgg ccgccccggt ggcgcgacgt tttggtatgg aacgtagcct
     5641 gtttgccgcg ttacttttga tctgtgcagg tatcgcaatt cgctctctcc cttcgcctta
     5701 cttattattt ggcggtacag cgatcattgg cggtgggatt gcattaggca atgtcttact
     5761 gccaggatta attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc
     5821 cctgacaatg ggagctgcgg cggcactggg atcggcgatg gtagtgccgc tggctttgaa
     5881 cggttttggc tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt
     5941 tttatggctg ccacagtggc gaagtcaaca acatgcaaat ttgagtacct cacgcgcctt
     6001 acatactcgg ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat
     6061 caactcactg gtctattacg tgattattgg ctggcttccg gcgattctca tcagtcacgg
     6121 ctatagcgaa gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc
     6181 cggtttgctg atcccacttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt
     6241 cgttgccttg atgtgcgcag tgggcgcggt tgggctctgc tttatgccag cacacgcgat
     6301 cacctggact ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt
     6361 cattggtctg cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc
     6421 cgtcgggtat ttgctggcag cctgtgggcc gccgctgatg ggtaaaatac acgatgctaa
     6481 cggtgactgg tccgtaccac tgctgggtgt tgcaatactt tcactactga tggcgatttt
     6541 cggactttgc gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga
     6601 caaatcatgc gtgaaaggga gaacaaatac gatgaatatt cagtgcaaac gcgtttatga
     6661 tccggctgaa cagagcgatg gttatcgcgt actggtcgac cgcctctggc cacgcggtat
     6721 caaaaaaacc gatttagccc ttgatgaatg ggataaagaa atcacgccgt caacggagct
     6781 gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc
     6841 agaactggcg caacacgagc aagaaggaaa gcggctggca gacatcgcca aaaaacagcc
     6901 gctgaccctg ctctactcag caaaaaacac cacgcagaac catgcgctgg tgctggcaga
     6961 ctggctacgt agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat
     7021 ggtcccgtcg ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt
     7081 tgctgacccc ttgcgctgtc tgcacaggaa tgagcgtccc gcccttctgc tggcaataga
     7141 ccgacgccgg atttgccata ccaatctgcg gcggtttagg tgcttctggc tgagaagggg
     7201 ttgaacaacc agccaggacc agcaagcaag gcagaacaaa actgataatt ttcatttatt
     7261 gatctcacat atttatccag gcttagagta tcgcggtatc gttttgtttt gcagcactat
     7321 ttttattaca ttcactcaaa acatattacg ccttgtttca tctttgttga tgatgtttta
     7381 tcatgcctgc aaagattaaa taatcagcat ttacccgccg tatcctggag ttgtttcgtg
     7441 tcagatcaga ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt
     7501 ctggttcgac agcttctgga gatgctggaa atggtcactg atatggagtc aacctacctg
     7561 accaaagtgg atgtcgaagc gcgcctgcag catataatgt ttgcccgtaa tagccagaaa
     7621 atgcacatcc cggagaattt taccgtctcg tgggattact cattatgcaa acgcgccatt
     7681 gatgaaaact gttttttcag cgatgaagtc cccgaccgtt ggggcgactg tgttgcggca
     7741 cgcaatcttg gcatcaccac atttctgagc acgccaattc acttaccgga tggatcattc
     7801 tatggcacgc tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag
     7861 gtcttacagt tgttcgccgg actgattgca caatatattc aaaaagaggc actggttgaa
     7921 cagctgcgcg aagctaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg
     7981 ctaccgaatc ggcgggcgat ttttgaaaat ctgacgacgc tgttttctct cgcccggcat
     8041 cttaaccata agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat
     8101 cgttttggtc ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg
     8161 ctccagcaaa atggcgaagt tattggtcgt ctcggcggtg atgagttttt ggttgtttca
     8221 ctgaacaacg agaatgcgga tatttcgtcg ctgcgagaac gtattcaaca acaaatacgc
     8281 ggagaatatc acttaggtga tgttgatttg tattatcctg gtgccagtct tggcatagta
     8341 gaagtcgatc ctgaaacgac cgatgcagac agtgccctgc atgctgccga tatcgcgatg
     8401 tatcaggaga aaaaacataa acagaaaaca ccttttgtca cgcattcagc gctacattcc
     8461 tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg
     8521 ttgtaatctt tattacagcg gtcgtatttt tagcatggtt ttttattggc ggctatgctg
     8581 ccccgggagc ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt
     8641 cgtactcggc actgagctgg gatggtggta acgtcacatc taaaaaatag caaaggctgc
     8701 ctgtgtgcag cctttgtgca atttacgcgt taacttttaa tcttcctgta gataaatagc
     8761 acaacaattg caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta
     8821 ccaaagccaa acagcgtgct gatccatccg ccaactacgg caccgactat ccccagtagg
     8881 atagtcataa agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg
     8941 gcaataagcc caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt
     9001 gcattagcgg gttagtcgct gataaaaagc atagcacaac atcgggaggg taagatttgt
     9061 gacgagcatc acggaggaat tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt
     9121 gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc
     9181 accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct
     9241 gtttcataat cgacagaaac gccattgctg ttattggtga cggtcacggt atacgttgct
     9301 ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcgcc ttccatcagg
     9361 acttcaggag caacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg
     9421 ttgtgccact tttctggagg gaacaccgca aactgccccg cttcgataag gatcacctga
     9481 tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg
     9541 gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc gaatattccg
     9601 gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata attctgtggg
     9661 atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta ttccagccgt
     9721 ttcagggtac gcctgataat ttgcatttta aataccattt attggttact ttttagcacc
     9781 gtatcagaga agaatcaggg aggattacag gtgggaagcc cgcggcaacg cgggctgaaa
     9841 gcatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca aaccaatcag
     9901 ggagttgccc actttagcca gtttcttttt ggtacgtatg tactgcgtga caaaagcccc
     9961 agaaataatc agaaagctca aatagcagaa actcaccagt tccagcgtcg tcgccagaat
    10021 aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga aaaacgacac
    10081 atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc
    10141 accgtattgg ggctcatcgg atttggcctc gctattttta cccttcaggg ttgcgtagag
    10201 aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacga tgttgaataa
    10261 tatcggggtg gtcttaatta atgtcgccac tccagcccat gccagaaaca tcaataccgc
    10321 atcgccaata aatacaccac aggccgcaag ataaccgcct ttcataccgc tactgacgct
    10381 atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa tggccccaac
    10441 cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct ctttaattat
    10501 ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg
    10561 agaaaatcag gttttaactc gatatcaacc cgataattga atcattaatc aggcatgctc
    10621 cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta ccgttatccg
    10681 cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacattcg ctggctggta
    10741 atattccggt agcacttgta ctaaatgacc actggcggga aacagcctgg cgacatcgat
    10801 ttttacgtgg tccggaaaaa caccgaaggc gaatattcct cgccaggcgg tagagtaaat
    10861 gaaggaacag agaatgaagt cgtcattcag gaatcggtat ttactcgtcg tggtgtcgag
    10921 cgcattttgc gttaagctca aataagttca tttgaaccac ctgctgttgg atttgtagtt
    10981 tgccgaagcc ggactgagcg cgcccagtcc ggcttcggaa gattttactg tggatattcc
    11041 tgcaataaat tgtgtaatgc ttccgccatc aactcaccac gccagccgga aatcagctcc
    11101 ggcaaattgt tctgcggttt cagtttccag tgccagttca gcagttggtt gatttgccga
    11161 cgcgacgcca gcaattcggc gctgatcttg tgcgtttcgc tgacgtcagt aatcagcgac
    11221 ttaatcgctt taaacgcttt acgataaccc ggcatgtcca tcaggttaag catcggctgc
    11281 ggtaaggcct cttctggcaa tgcctgcgct ttttccacca gcgccagcaa cgttttaccg
    11341 tggaagcgga tttcgctacc ggataagccc aggctgtcca gttcgcccag acttcccggc
    11401 atataacgcg ctaccgacca caaatgctct tcacgcacga caaagttcac cgccagatcg
    11461 cgctctcgcg ccttgcgcaa tcgccagtcg gctaacagtt gcagacaggc cagttggcgt
    11521 gtgcgtaact gccaggcatt ggtgatatca cgccaggcgt cttccggcgc aacgacttcc
    11581 tgacgacgca tttgcatcag gcggcattca tccagcgccg caggtagcca gccggaggcc
    11641 tccgtttcta ccataagctt ggcggtgatc ggtaacagat accagacatc cgccgctgca
    11701 tattcacact gacgttcggt cagcggtctg gccagccagt cggtgcgcga ttcactcttg
    11761 tccagcgtaa cgccggaata ctcttccacc atggaagcga aaccccatga catcgggcgt
    11821 ccgcagaagg cagcaaggat ttgcgtgtca atcaggggtt gtggtaattc gccaaagaca
    11881 ttgaggaaca cttccagatc ttcactgcct gcatggagaa attttgtgat ggacggatcg
    11941 cgcaggatcg ctttcagtgg tgaccagtcg gagatcccga gaggatcaat tagcgccaga
    12001 tgctcaccat caaaaagttg aattagaccc agttgcgggt aataggtgcg cgtacgaaca
    12061 aattcagtat ccagggctat cgccggaaag gcacggacgg cttcacacaa agaagccagc
    12121 gcatcgtccg tggtaatcat ttggtaattc aaattgtatt ctctttagtg ggcgtcaaaa
    12181 aaaacgccgg attaaccggc gtctgacgac tgacttaacg ctcaggcttt attgtccact
    12241 ttgccgcgcg cttcgtcacg taattctcgt cgcaaaattt ttccgacgtt agatttcggt
    12301 aactcatcac gaaactccac cagcttcggt actttgtagc ccgtgagctg acggcggcaa
    12361 aaggtcacca gtgactcttc ggtaagcgat ggatcttttt tcactacgaa gattttcacc
    12421 gcttcaccac tggagccgga aggtacgcca acagccgcga cttcctgtac gccaggatgc
    12481 tgcatgacga catcttcaat ctcgttggga taaacgttaa aaccggaaac cagaatcatg
    12541 tcttttttac gatcgacaat gcgcaggaat ccttcttcat ccatcaccgc gatgtcgccg
    12601 gtgtgtaacc agccattttt gatgatttca tcggtagcat ccggacgctg ccagtaaccc
    12661 agcatcacct gcggtccttt gacacaaagc tcacctggtt gaccaggtgg tacttcatta
    12721 tcatcatcat ccaccagttt ggcttccgtc gacggcaccg gcaaaccgat gctaccacta
    12781 tgataatcaa tatcatacgg gttaacgctg accagcggcg cgcactcggt caggccataa
    12841 ccttccagca gatactgtcc agtcagttta acccaacgtt ctgccaccac ttgctgaaca
    12901 ggcatccctc cgcctgcgga aagatgcaga ctggagaaat ccagctgctg gaactcttta
    12961 ttgttcagca acgcattgaa caaggtgtta acaccagtga tagcggtaaa cggatatttc
    13021 gctaactctt ttaccaaccc tggaatatcg cgcgggttag tgataagcag gttctgcccc
    13081 cccagttcga taaacagcag gcagttaatg gtcagtgcaa aaatgtgata cagcggcagc
    13141 gccgtcacca ccagctcttt gcccggatgc aacagcggac cataggtcgc gttaacctgt
    13201 tccaggttcg ccagcatatt gcggtgagtc agcatcgcgc ctttcgccac accagtggtg
    13261 ccgccggtgt attgcaggaa agctaaatct tccggcacca gttctggttt gacgtactgc
    13321 atccggtagc cgttgtgcag ggcgctacga aatgaaatgg catctggcag atggtatttc
    13381 ggcacaagac gcttgatgta tttaacaacg aaattgacta ccgtgccttt tgccgtagat
    13441 agctgatcgc ccatacgggt cagaataacg tgctgaacgg cggttttatc aaccactttt
    13501 tccagagtgt gggcaaagtt agacacgata acaatcgccg atgcgccgct atcgttaagc
    13561 tgatgctcaa gctcacgcgg ggtatacaac gggttaacgt ttacgacgat catcccggca
    13621 cgcaaaatgc caaacagcgc caccggatat tgcaataagt taggcatcat caacgcaacg
    13681 cgatcgcctt tcttcagccc caacccttgt tgcaaataag cggcaaacgc gcgactgcgt
    13741 tcttccagtt tgcggaaggt catcacctcc cccatattca caaacgcggg ttgatcggca
    13801 tagcgcgcga ccgactgctc aaacatatct accagagatt gataacggtc agggttgatc
    13861 tccgtcggaa cgtccgtggg ataacggtta agccaaacct tcttcaattc ttcacctcta
    13921 aaatgcgtgt tcgtcgtcat cgcaacccca agtgatagac atgctgttaa cataatatta
    13981 actcatctta ccagcttgat aattacccaa ggaaaaggtt gcgaagcgcg tcactattta
    14041 tttttatctt taccgtaaga atgcagaaac agcggaccgg ccgctgtttc tttttcttta
    14101 aaaacaagca attattcagt tacaactgtt tgtactctcg cggggccggg attataccag
    14161 ccccatccac cgtagccata gggccagcca cgaccgccat aaaaccatgg atcaatcggc
    14221 tgaggcggca taatcacctg ctgggttaaa tgccaacgtt tgtaacctgt tgcctgcatc
    14281 accataaatt tatagggcgt gttgccgatt tttccgtcaa ccgtaccggt gattggcccg
    14341 acaactgtca ccagctgccc acggaaatcg accggatcaa gaaaaccatt cacatcggca
    14401 taaatgcgac cgcgagaagg ttctcccagc gtcggtctgg caccgctgtc cagcggtacg
    14461 gtagcaattt ccaggcgggt tttcccttgc tggttttgta ccgcaaccac tttgccgcca
    14521 aagcgtgcct cctgaccaac gtacagctgc ggcgcactca tcacccgaac taaatcttgt
    14581 tgcggcgtgg gactgctgcc tttaatggcg tccggcacag taacacaacc gctcagcatt
    14641 agcgcaaacg tacctgccag tatgcctttg ataacattct tttgaaccgc catgatgcga
    14701 ctcctttttt tctcagggca tactcttaag attcattctt tgcccggaag tttcttccat
    14761 gcgacgttgt tacgtaaata aactggttca gcatgttcca ccgccaccgt tttaccctcg
    14821 gcaaacatct ggcacgcaat cggcagcata tcttctgcag cgggcagtaa cacttcgcca
    14881 tcgcgcaaaa ccagcccgct ctctttaccg agatccggcc aggcttgcca gcccgtccct
    14941 accgtcaccc attcgccgga aagctgttgc attcgttcat gaaccagttc gggtttgagt
    15001 acggcttcgg tttcttcacc atgccagata ccgttttcat cacgctgata ttcggcccag
    15061 taaacttcgc ccattcgcgc gtcaatagct gccagcacgc gggttgcgcc gtttttgcgc
    15121 cacgcctctt gcgccatcgt cattagcgtg gagacgccaa tcatcggcag ctctgcgcca
    15181 agtgccagcc cttgcgcaat gccaatacca atgcgcacgc cggtaaagct accggggccg
    15241 cgcccgtaag ccagagcgtt tatgtcagtc agagaagttc cgctggtggt caggatatcc
    15301 tgcaccatcg gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg
    15361 acagtaccgt cgttccacag ggcgacagag caggcctctg tcgcggtatc gatagccaga
    15421 attcgcatgg gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc atactccgta
    15481 acaaattacc tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat gtcgcgggtg
    15541 cgcggcgctg gcggcagact ggcgagaaac gtcgcgccgt aagggcgcat caccagccga
    15601 ttgtcgcaaa tcactaacac gccgcgatcg tcggcgtcgc gaatcaatcg ccctaccccc
    15661 tgcttgagag taatgaccgc atccggcagt tgtacttcat cgaacggatc accaccgcgc
    15721 aaacggcaat cttccatgcg tgcttttaat agtggatcat ccggcgaggt aaacggcaat
    15781 ttgtcgataa tcaccaatga caatgtatcg ccacgcacgt ccaccccttc ccagaaactg
    15841 ctggttgcca caagaagcgc gttaccggcg ctgacaaatt gctgcaacag ttgccctttg
    15901 ctggtttccc cctgcaacaa aacgggaagc gtcatggtag cgcggaactg ctcggccaga
    15961 tcgcgcatca tggcgtgcga ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg
    16021 atcggtcgca gcattgccgc cagttgccgt gcggaacccg gttgattggt ttgcggcagg
    16081 ttgcgcggca cacagagtaa cgcctggcgg ctgtaatcaa aggggctggg taacagcaac
    16141 gactctgcct gttcaatgcc aagccgcgag gtgaaatgat gcagatcgtc gtttaccgac
    16201 agcgttgctg aggtaaagat ccagctaccg gttttttgcg ccattaactc tttgaattta
    16261 tccgccacgc tgagcggcgt gagagccaga gtaaaatggc gcgaagtgca ttcgtaccag
    16321 tagctgtagc ccggctgatt gatctctttt agccgtttca gccgtgtgcg atataacgtg
    16381 gcgcgttcaa atgccgcatc cagcaaggcg gaacgcccca gcgacagttt cgccacgtca
    16441 taacaaagtt ccagagtgtc atcgagcagt aaaaatgccc gctgaatttg cggattagct
    16501 aacagctcac gcaggttacc gcgataacct ggctcaccga gttgcaggcg aaaatcctgc
    16561 gcactctggg caagacggtc cgcgcacttt tgtaactgct gggtgtcttt taattcggtg
    16621 cggtaggcga tggtgatgtc ttttgccagg tcgagcagtt gtcgactgga gagtgactga
    16681 ccaaaatact ggctggcaat gtcgggcagc tggtgggctt cgtcgaagat catgacgtcc
    16741 gcttccggga tcaattcgcc aaaaccgctc tctttcacca ccatatccgc cagaaacaga
    16801 tgatgattca ccaccaccac atcggcgtcc atcgcttttt tgcgtgcttt gaccacaaag
    16861 caatctttat acatcgggca gtcactgccg aggcagttat cgttggtgct ggtgaccagc
    16921 ggccacgcct gtgaatcttc cgccacgctg acgcaggtgc tgatatcgcc atcgactgtt
    16981 tgattagacc aggagcgcag caggatcaca tcgcttaaga tttgtaccgg cagatcgccc
    17041 cccgccagcg cctgctgttc gagacgttcg aggcagaggt agtttgagcg ccctttcagc
    17101 agcgccacat tgcccgtgta tttcaatgcc tttgagaccg ttggcaaatc gcggctgtag
    17161 agctgatcct gcaacgcttt tgagccggtc gagattatga ctttcttttt cgcccgcagc
    17221 gcaggggcca ggtaagcgta ggttttgccc gtaccggttc ctgcttccac caccagcggc
    17281 tggccttttt ctatcgcctg ggtgacggct accgccatct gtcgctgtgg ttctcgcggc
    17341 ttaaagcctg gtatcgcttt cgctaactga ccgtctggtg caaaatcgtc cgtcacacta
    17401 ccccctgttg atttgaacag ggattatgtc aggatgaggg cgctttcgcc agttgaagtg
    17461 gtgacggcga cctcacattg tggcagtctt tgcagcacga aaatgggagt ataatgagga
    17521 caaaatgatg actatcgttc gtatcgatgc cgaagcccgc tggtctgatg tggtaatcca
    17581 caacaacacg ctctactaca ctggtgtacc ggaaaacctc gacgccgatg cctttgagca
    17641 aaccgccaac acgctggcgc agattgacgc cgtgctggaa aaacagggca gcaataaatc
    17701 gagcattctg gatgccacca ttttcctggc cgataaaaac gacttcgcgg cgatgaataa
    17761 agcgtgggat gcctgggttg tcgcgggtca tgcgccggtg cgctgcacgg tacaagcagg
    17821 tttgatgaac ccgaagtata aagttgaaat taagattgtg gctgcggtgt aagctttatc
    17881 gaagcaaaat aagtcagacg ataatttatc gacaatactg gtcggttttg cataaatcga
    17941 ccagagatt
//
LOCUS       DAZFRE010000098        17590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFRE010000098 DAZFRE010000000
VERSION     DAZFRE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.098"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            219..2003
                     /locus_tag="ACHKUI_003930"
     CDS             219..2003
                     /locus_tag="ACHKUI_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFO6600028.1"
                     /translation="MWSDKESSEDYLNFGEVSQLAVDVLTTKDMLPVSIGIFGNWGAG
                     KSSLLKLIEQKLEQDDKDWIVINFDSWLYQGYDDTRAALLEVIATELTKAAEGNSALI
                     SKTKRLLSRVDGFRAMGLLAEGTALMAGLPTGGLLSRGIGALRNITDGIQSQEEYEAL
                     GNIAKEGKETACGLIKPQTKKSPPQQIDAFRKEYGEILEELGKPLIVVIDNLDRCLPA
                     NAIHTLEAIRLFLFLTNTAFIIAADEDMIRSSVADYFKGASQRHQIDYLDKLIQVPIR
                     VPKAGVREIRSYLFMLYAIEHGLEGEKITMLREGLEKALQQSWKDEPISRQEALKMTG
                     EADDSNLALAFARADRIAPILANSPIIHGNPRIVKRLLNVVKMRSQIAKRRAMPLDEA
                     IITKLVIFERCVGVDGTADLYHLVDIEQGVPQILKQLDDNGGQIPTDAPKTWTDSPTT
                     KSFISQWAQLEPRLGGIDLRAAIYLSRETMPIGAYVVGLSPSGREVLNALIELKNTSS
                     PTAENLLKALPREEQIPVMEGLINQLRQVSDWDRKPRGFSGACLLARYSTDAASILIR
                     YLQELQLGMKRPAWMTAALKDEQWNKDA"
     gene            2003..2755
                     /locus_tag="ACHKUI_003931"
     CDS             2003..2755
                     /locus_tag="ACHKUI_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600029.1"
                     /translation="MGTSQSSKGPGGGSPLVPPWADDQPQQPLPSPQERRFAPFRESL
                     GNAVSNGNRADFRKAIGHYARKASGGSSNAARRLGSVTQAGAELFGALVGMPSAPGEP
                     SIDLGSLAGLPCEIAISTIAQALTSQDGDSEKICAAMNHALVEALDGVEIFDPQKITD
                     GVIVDTMIGYLAESIFLQMVMDSNRAWNKADTPSKAIHAEIELRELIKVVVDKHMAPK
                     LAGNIRSFTRNQMVKIERQAIIEAWQEWEAYQ"
     gene            2737..4056
                     /gene="qatC"
                     /locus_tag="ACHKUI_003932"
     CDS             2737..4056
                     /gene="qatC"
                     /locus_tag="ACHKUI_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system QueC-like protein QatC"
                     /protein_id="HFO6600030.1"
                     /translation="MGGIPVTQLVFHHKHHHLPPASEKVLPVQLYGLSGQRRGDISVI
                     GNPAIDRIRRLGVQLPAKVMDFLSVALAVTAADTFVQRESSEDGWTRQLSLRLPLHEP
                     SRWISLKKELESALHFLSGDIWDFEFCDDGYAPPEPYSQHSRHRLIKLKGLDCVSLFS
                     GGLDSAIGAIDLLAAGRAPLLVSHAYKGDKSRQDQIAEKLSGQFSRFEINADPHIYQG
                     VTDITMRTRSLNFLAFAAVGACAVQEISQQEKIDLFVPENGFISLNAPLTPRRIGSLS
                     TRTTHPHFITSIQKIFDALGISCQIINPYQFKTKGKMISECSNKQLLSKIVESTVSCS
                     HWKRMGQQCGVCIPCIIRRASLHAGGISRDVEYIFQSLAKVMNEIDRRDDLIALRIAI
                     TQKSTLKIGTWIAKSGPLPTAEFDNFKQVFKDGLDEVESYLLSENIV"
     gene            4053..4778
                     /gene="qatD"
                     /locus_tag="ACHKUI_003933"
     CDS             4053..4778
                     /gene="qatD"
                     /locus_tag="ACHKUI_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system TatD family nuclease QatD"
                     /protein_id="HFO6600031.1"
                     /translation="MSIDMHCHLDLYPRPDLVAEESKRRGTYILSVTTTPKAWHGTSL
                     LAKESQRIRTALGLHPQIAHQRSHELDLFDSLLSETKYVGEIGLDGGQGFKEHWDIQL
                     KVFRHILNSVNRAGGKIMTIHSRGSASAVLDEIENIDGVAILHWFTGTPKQLERAIDL
                     GCWFSVGPAMLDTIKGKALVLKIPKSRILTETDGPFAKFRNDPLMPWDSGIAEKQLAA
                     LWGISQMEVNAQLVDNFKVLCTS"
     gene            complement(5331..5906)
                     /locus_tag="ACHKUI_003934"
     CDS             complement(5331..5906)
                     /locus_tag="ACHKUI_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFO6600032.1"
                     /translation="MTQSEMLKLIARHGYNVGFGAKKNFATYDLICKASGWISFISLA
                     VGVFALFIPELATNVISAILIIFGVATMYIQFYDSEKQSYESAGIEQTKIFNELEVIY
                     RNVKSDANFDYQRTQQSIDAIMSRFYGTVISKQIFGSNWYAHYKFYCEFEKAWVEEEL
                     NLTLKDKIPLSLCFLASLAIIFTIAVIWKTP"
     gene            complement(5914..7242)
                     /locus_tag="ACHKUI_003935"
     CDS             complement(5914..7242)
                     /locus_tag="ACHKUI_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFO6600033.1"
                     /translation="MSHRELFSEFLENLNLDLKQAKKISYHYRKITKSLNLAFRGTSS
                     RVANRLKVGSVGRHTAIKGISDLDMLYIMPPNQYEYYNRKDNGQSALLTDVRNILAEE
                     YPDQTVKKDRLVVQIIFKNFYVEVQPVFRQDDDSFKFPESYNGGAWRITKPLHEKAAM
                     TAFSRDKSNNLRKLCKMIRAWKNLHGVNMGGLLIDTLAYRFLSSTSDYDNTGNGSLGA
                     LARDFFEYLSNEERKERYLALGSNQHVRVKSPWFGRAAKHAYELCCDALDAEGAASEN
                     DRWRKVFGRAFPRRKVGIMEARLGLESHAADAVPWTDTEEFIEDKYPVDIRYSLNLDC
                     TVTQDGFPPRSLREMLIRRFRLSARKSLLFRADLTEMEAEEPYTVMWKVLNVGDEARR
                     RNMIRGQIVSDGGYCTKKETTDFRGDHMVECYVIKNEVVVARAQIEVPIS"
     gene            complement(7377..7562)
                     /locus_tag="ACHKUI_003936"
                     /pseudo
     CDS             complement(7377..7562)
                     /locus_tag="ACHKUI_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7583..8739)
                     /locus_tag="ACHKUI_003937"
     CDS             complement(join(7583..8479,8482..8739))
                     /locus_tag="ACHKUI_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6600034.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKGVLRKAVRLRYAFIRD
                     NTCCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRK
                     IHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIMSPNRLQRQFNPDA
                     PDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRN
                     PEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIK
                     KKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            complement(8812..9165)
                     /locus_tag="ACHKUI_003938"
                     /pseudo
     CDS             complement(8812..9165)
                     /locus_tag="ACHKUI_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(9783..10476)
                     /locus_tag="ACHKUI_003939"
                     /pseudo
     CDS             complement(9783..10476)
                     /locus_tag="ACHKUI_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            10729..12198
                     /locus_tag="ACHKUI_003940"
     CDS             10729..12198
                     /locus_tag="ACHKUI_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFO6600035.1"
                     /translation="MHIDSTEKNNKQKLGLLALTSIVVSSMIGSGVDGLPQNMAENSA
                     VGPVAIAWIVCGIGMFFISRTFIVLSNIRPDLQSGVYMYAREGFGRFTAFNVAWGYWL
                     MTIFSNVAFAIMVMDTLNYFLPGDFKGGNNIPSVIGASLLIWGFNFLVLSGTRIAGSI
                     NIVGTLAKLIPLVAFIIILMLFMKYTTFADNVWGHSASKASQDLGSIFSQVTSPLSVA
                     LWSFIGVEGAVALSGRAKNKRDVGKATFIGFVVSLIICLLVSILPFGVLTQHELSAIP
                     TPSTAGILKIVVGEWGKIMIILGVLISVLTSWLAWTIICAELPMVAAQNGTFPKPFSK
                     KNVKGSASVSLYISSAFMQIVVLLVYFSNDAWLAMLAISTLTVLPAYLMTTAYLFKLC
                     ISGEYASYTTNHSRSAALVSSIIGFVFCLFMFYASEIKYLAMVPVLLTLGVPLFIWAR
                     KKDDRADKIFTLRELAFFILLLVLDIIVFILLHYKTIHF"
     gene            12303..14672
                     /locus_tag="ACHKUI_003941"
     CDS             12303..14672
                     /locus_tag="ACHKUI_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HFO6600036.1"
                     /translation="MKFNHNLLFISSQYLDGDNPSQQVLEELQTELAERGFKIHITHQ
                     ISDGLKIIEKSPQYSGIGFYWEPDNPTFAEELQHFISIFRKRNATTPLIIFSEQNITD
                     RIPLDVLKEVSEYVYLFSESAAFTANRLYSLVHRYADKLLPPYFKTLKDFTEDGDYYW
                     DCPGHMGGMAYLKHPVGIEFINFFGENMMRADIGVATAEMGDYLIHAGPPKKSEEIAA
                     RLFGSDWTFYGVSGSSGSNRIVAQAAVGADEIAIIDRNCHKSLNHGLTLSQARPVYLK
                     PTRNAWGLIGPIPTGRLKKASIDALVANSRLASGAVSQSPSYAVVTNCTYDGFCYNVN
                     DVVRHLGESAPRIHFDEAWYAYARFHPLYQSRYAMDAEETPNRPTLFAVQSTHKMLPS
                     LSMASMIHVKKSDRAPLNFDDFNDAFMMHGTTSPYYPIIASIDVAVSMMEGESGYSLV
                     QESIEEAIAFRKAVVSVKRQLQEQEGGDAWFFDVLQPTEVQDSDSGQRYSFEEAPVSL
                     LSHSADCWSLRSGERWHGFADDDLVETNSMLDPVKVTLTCPGIGPKGEYQKNGIPGYL
                     LTRFLDDRRIEIARTGDYTVLILFSVGITKGKWGTLIESLLAFKKHYDNDDLATDAIP
                     SLKAHSPHYDTLTLKELCQIMHEKMDELELMSHINDAVNTDPEPVMTPAEAYQKVVRY
                     KTEHIRLDDFSGRIAASMLVPYPPGIPVLMPGERMPQGNKGIIGYLRALQEFDKQFPG
                     FEHEIQGVNVDEDGDFWVRAIVEEERDGQSLPGHITFKRQVSGIKKGRQ"
     gene            14747..16246
                     /locus_tag="ACHKUI_003942"
     CDS             14747..16246
                     /locus_tag="ACHKUI_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFO6600037.1"
                     /translation="MAEQSSDVKNSDNSAGGHKLGLISLIAIVVSSMLGGGVFSLPQN
                     TAASSALGPVIIAWIIAGIGIYFIANSFRVLSDLRPDLQAGVYMYAQEGFGSFIGFNV
                     AWGYWLMTAFGNVAFAVILMDAFNQFMPGVFTDGNNLNSIICGSVLIWGYNFLVLSGT
                     KVAGFVNTLGTIAKLVPLILFVLLLGVLIDYSDLFKNFWGESPAILSGNGNDAAPVSL
                     LSQILAPMTVTLWAFIGVEGAVVLSGRAKNKQDVGKATLIGFLLALIIYMLMSILPFG
                     VMPQAELAQVSNPSTAGVLAHVVGQWGDWLMSAGLIISVLAGWLAWTMLCAEIPMVAG
                     QKGAFPRAFARTNSKQAANVSLWVSSFVMQAAMLLVYFSNNAWTTMYNISALMVVPAY
                     ITTTLYLTSICRNGKYDQYAKKGRRLALSSGVLGSLFCLFILYASQIEYVALIPILLT
                     CGLPVFIWSRKEKNDGQPIFTKKELIYLIMLLLCDIIVVALFIGGYLKV"
     gene            16389..16664
                     /locus_tag="ACHKUI_003943"
     CDS             16389..16664
                     /locus_tag="ACHKUI_003943"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFO6600038.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHLKNSGRSR"
     gene            16709..16900
                     /locus_tag="ACHKUI_003944"
     CDS             16709..16900
                     /locus_tag="ACHKUI_003944"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO6600039.1"
                     /translation="MDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERL
                     LSLLSAFEVVVWDGWLAAV"
     gene            16860..17078
                     /locus_tag="ACHKUI_003945"
     CDS             16860..17078
                     /locus_tag="ACHKUI_003945"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO6600040.1"
                     /translation="MRSWYGTDGWPLYESRLKGKLHVISKRYTQRIERHNLRQHLARL
                     GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(17264..>17590)
                     /locus_tag="ACHKUI_003946"
     CDS             complement(17264..>17590)
                     /locus_tag="ACHKUI_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6600041.1"
                     /translation="DPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGL
                     WSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREQCISPFEYAK
                     INNPLK"
BASE COUNT         4570 a         4022 c         4186 g         4812 t
ORIGIN      
        1 tttgacaaga gcgaatttaa cccttcggat tctgatagga aaatatggac tgggataaga
       61 aagcttattt cattctttaa aaaatagatc tatagcagtc atcatattgg attattggtg
      121 aagtggtaca ctgaatttgc ccacctgaac agagttggtt ttatcaaact tgtagtttac
      181 tcaatgacgt aaaaattggt gatgtaaagg atataaaaat gtggtcagat aaagagtcat
      241 cagaagacta cctaaatttt ggtgaagtat ctcagttagc cgtggatgta cttaccacga
      301 aagatatgtt accagtatct atcggaattt ttggaaactg gggggcaggt aaatcctctc
      361 tgttaaaact gatagagcaa aaacttgagc aagacgacaa agattggatt gttatcaatt
      421 ttgactcttg gctctatcag gggtacgacg acacccgtgc cgcacttctt gaagtcatcg
      481 ctacagaatt gacaaaagct gctgaaggta attcagccct tatatcaaaa actaagagac
      541 tccttagtcg agttgatggt tttagagcta tgggattact agctgagggt acagctttaa
      601 tggcaggatt acctactggc ggtttgcttt ctagggggat tggtgcatta agaaatatca
      661 ccgatggcat ccagagccag gaagagtatg aggctttagg caatatagct aaagaaggta
      721 aagaaactgc ttgtggtttg attaaaccac aaacaaaaaa aagcccccct cagcagattg
      781 atgcctttcg taaggaatat ggggaaattc tagaagaact tggaaagcca ctcattgtgg
      841 taatagataa cctagaccgc tgtctccctg ccaatgctat ccatacactt gaagctatca
      901 ggctattcct tttcttgact aatacagcct ttattattgc agcagatgag gacatgattc
      961 gctcttctgt ggctgattac ttcaaagggg catcacagcg ccatcaaata gattatctgg
     1021 ataagctaat ccaggttcct attcgggtgc ctaaggctgg ggtccgtgag atccgttcgt
     1081 atctgttcat gctttatgcc attgaacatg gcttagaagg cgaaaaaata actatgctcc
     1141 gtgagggctt agagaaggcg ttacagcaat cctggaaaga tgaaccaatc tcacgtcagg
     1201 aggccttaaa aatgactggt gaagcggatg atagcaacct cgcgctggcg tttgcgcgtg
     1261 ctgaccgtat tgctcccatt ttagccaact ctccaattat tcatggtaat cccaggatcg
     1321 ttaaacgctt gttgaatgtt gtgaaaatgc gatctcaaat tgcgaagcga cgagcaatgc
     1381 ctttggatga agcaattatt actaagctag taatttttga acgctgtgtt ggagtggatg
     1441 gcaccgctga tttatatcat ctcgtggata ttgaacaagg tgttccccag atacttaaac
     1501 agcttgacga taatggcggt caaataccta ctgatgcacc aaagacatgg actgatagtc
     1561 caacgactaa atctttcatc agtcaatggg cccaacttga acctcgtctt ggtgggattg
     1621 acttaagggc cgccatatat ctgtcccgag aaactatgcc aataggtgca tatgtggttg
     1681 gtttatcgcc atctggacgg gaagtactaa atgcactaat tgaattgaaa aacactagtt
     1741 ctcctacagc agaaaacctt ttgaaagcac ttcctcgtga ggagcaaata cctgtaatgg
     1801 aaggtttaat taaccagtta cggcaggtat cagattggga tcgtaagccc agaggctttt
     1861 ccggtgcatg tctgttggcc cgctactcaa cagatgcagc cagcatatta attcgttatc
     1921 tacaggaatt acagttgggg atgaaacgac cagcgtggat gactgcagca ttaaaagatg
     1981 aacaatggaa taaggacgct taatgggaac atcacaatca agtaaaggtc caggaggtgg
     2041 ctctccgctg gttccaccat gggctgatga tcagccacag caaccgttac cctcgccgca
     2101 agaaaggagg tttgcgccat ttcgagaatc gttgggaaat gcggtatcaa atggaaatcg
     2161 agcagatttc agaaaagcca tagggcacta cgcgcgaaaa gcctccggag ggagcagtaa
     2221 cgctgctcgg cgattaggga gtgtcacgca agctggggcc gaattatttg gggctttagt
     2281 gggaatgcct tcggctcccg gagaaccaag catcgatttg ggcagtttgg caggccttcc
     2341 atgcgaaata gcaatatcaa ctattgctca agctttaaca tcacaggatg gtgactcaga
     2401 aaagatctgt gcggccatga accatgcttt agtggaggct cttgatggcg tagaaatttt
     2461 cgatcctcaa aaaataactg atggtgtgat tgttgacaca atgattggtt atctagcgga
     2521 aagtattttc cttcagatgg taatggattc taatagggca tggaacaaag cagatacacc
     2581 ttcaaaggca attcatgcag aaattgaact ccgggaattg attaaagttg ttgttgataa
     2641 acatatggca ccaaaacttg ccggtaacat aagatcgttc acacgaaacc aaatggtaaa
     2701 aattgaacgt caggccatta ttgaggcctg gcaagaatgg gaggcatacc agtgacacaa
     2761 ttagttttcc atcataaaca tcaccatttg ccgccagcaa gtgagaaagt gttacctgtt
     2821 cagctatatg gattaagtgg tcagaggcgc ggagatatat ctgttatcgg gaatcctgcg
     2881 attgatcgga tcagacgttt gggagtacag cttccagcta aggtcatgga ttttctgagt
     2941 gttgcattag cagtaactgc agcagatact ttcgttcagc gtgaaagttc cgaggatggt
     3001 tggacccgcc aattgtcgtt acgactcccc cttcatgaac catccagatg gattagtcta
     3061 aagaaagaac ttgagagtgc tttgcatttt cttagtggag acatctggga tttcgaattt
     3121 tgtgacgatg gttatgcacc gccagagcct tatagccagc attcaaggca tcgtctgatt
     3181 aagctaaaag ggcttgactg tgtcagctta ttttcaggag gtctggattc agctattggt
     3241 gcaatagatc ttctggctgc agggcgcgct ccacttttgg ttagtcatgc ttataagggg
     3301 gacaagtctc gtcaagatca gatcgctgaa aaattaagtg gccaattttc gcgctttgag
     3361 attaatgctg acccacacat ttatcaaggc gtgactgata ttacgatgcg aactcgtagc
     3421 ctcaattttc ttgcctttgc ggccgtaggt gcttgtgccg tacaagagat atctcaacaa
     3481 gaaaagattg atttgttcgt acctgaaaat ggatttatct cattaaatgc accacttact
     3541 ccacggcgga taggttcgct gagcacacga acaacacatc cacattttat tacgagcata
     3601 caaaagatct ttgatgcgct cggtatttct tgtcaaataa tcaatccata tcagtttaag
     3661 acaaaaggaa aaatgatctc cgaatgttca aataagcagc tcttatctaa aattgtggaa
     3721 agtacagtat cctgcagtca ttggaaacga atggggcagc aatgtggggt atgtataccg
     3781 tgtatcattc gacgagcatc acttcatgca gggggaatta gtagagatgt tgaatatatt
     3841 ttccagtcct tagctaaagt aatgaatgaa atagatcgca gggacgacct gatcgccctt
     3901 aggattgcga tcacgcagaa atcgactttg aaaataggta catggattgc caaaagtggc
     3961 cctttgccta cggcagaatt tgataatttc aagcaagtat ttaaggatgg cctagatgag
     4021 gttgaaagct atttactgag tgagaacata gtatgagcat cgatatgcac tgtcatctag
     4081 acttatatcc tcggccagac ctcgtggctg aagaaagtaa gcgtcgaggg acttatattc
     4141 tgtcggtgac aacaacacct aaagcatggc atggtacttc tttattggct aaagaaagtc
     4201 aacgaatccg aactgctctt gggctacatc ctcaaatcgc gcatcaaaga tcgcatgagt
     4261 tagacctgtt tgattcattg ctttcggaaa ctaagtatgt aggggaaata gggcttgatg
     4321 gtggacaggg atttaaagaa cattgggata ttcaattgaa agtgttccga cacattctca
     4381 acagtgtaaa tcgggctggt ggcaagatta tgactatcca tagtcgggga agtgcatcag
     4441 cggtgcttga tgagattgaa aatatcgatg gggtggcaat attgcattgg ttcactggaa
     4501 cacctaagca gcttgaaagg gcaattgatt taggatgctg gttctcagtg gggcctgcta
     4561 tgctcgatac aataaagggt aaggccttag ttttgaaaat acccaaatca cgcattctta
     4621 cagaaacaga tgggccattt gctaagtttc gtaatgaccc actaatgcca tgggatagtg
     4681 ggattgcaga gaaacagtta gccgcattat gggggattag tcagatggag gttaatgctc
     4741 agctagttga taattttaag gtattatgta catcataaga atgaaaaact tagatatgca
     4801 tttacagttc aattcatttt tcgtcatcag ttaattacac ataaaattaa aagtaagaat
     4861 atatctaccc tgtgaatgag taaggcggat ttatatagtt tgtaattagt ttaaatgtaa
     4921 gcagttcgtc agagtgcgta ttccgctcta ttcgatcacg gattggccgt tatgacccga
     4981 tctcgatgga agttattggg tgatcccctt ccatgaaaat atgcaccgaa gctcccgcag
     5041 aggtttccgt gggcgaaggc atgattctgt ccgagagccg gatgtgttag aagcgcatgt
     5101 ccgcttcgat gagcggggtg tggaaacagg atttatggtc aggacagtaa ggcaccgcca
     5161 atcgaaagag gcggcaacag atatgcctga cctgtaaaat taccgcgcca catcttgact
     5221 ctacccgttt tccaagcgcc cccccttata gaacgtacac tggatggtga taccggctat
     5281 acatcgcata tttgacagtc agcttgctga atttttcatt ggctgcagca tcagggcgtt
     5341 ttccagatga cagctatagt aaaaatgatc gccagactgg cgagaaagca gagcgaaagc
     5401 gggattttgt ccttcagtgt caggttcagc tcttcttcaa cccatgcttt ttcaaattca
     5461 cagtaaaatt tataatgtgc gtaccagtta cttccgaaga tctgtttgct gatgaccgtg
     5521 ccgtaaaacc gggacatgat agcgtcgatg gattgttgtg tacgctgata gtcaaagttt
     5581 gcatcgcttt ttacgttgcg gtagatgact tcaagttcat taaaaatttt ggtctgctca
     5641 atccccgcac tttcataact ctgcttttcc gagtcataaa actgaatgta cattgtagca
     5701 acaccaaata taattaatat cgcggaaatg acgtttgtgg ccagctcagg aataaaaagg
     5761 gcgaacacac cgacagcaag agaaataaag ctaatccaac cggatgcctt acaaatcaga
     5821 tcataggtgg caaagttttt cttagcgcca aatccaacgt tataaccatg tcgggcgatt
     5881 aatttcagca tctctgattg cgtcataaaa acctcagctt atgggaactt caatctgggc
     5941 ccgggcaacc acaacctcat tttttatcac atagcattct accatgtgat ctccgcggaa
     6001 gtcggtagtt tctttcttcg tacagtagcc gccgtccgaa acgatttgtc cccggatcat
     6061 atttcttctg cgtgcttcgt cacccacgtt cagtaccttc cacataacgg tgtagggttc
     6121 ctctgcctcc atttctgtca agtctgcccg gaaaaggaga gattttcgag cggagaggcg
     6181 gaatcttctg ataagcatct ccctcaggct tctgggaggg aaaccatcct gggttacggt
     6241 acagtcaagg ttgagtgagt acctgatatc caccggatat ttatcctcta taaactcttc
     6301 ggtatcggtc cagggaacag catctgccgc atgcgattcc agaccaagac gggcttccat
     6361 aatacccact ttacgtctgg ggaaggctcg tccgaacact ttgcgccagc ggtcattttc
     6421 actggcagcc ccctcagcgt ctaaggcatc acaacataat tcatacgcgt gtttcgctgc
     6481 ccggccaaac cagggagatt taacacgtac atgctgatta ctgcctaacg cgagatacct
     6541 ttcctttctc tcctcatttg acagatattc aaaaaagtct ctggcgaggg ctcccaggct
     6601 gccatttccg gtattgtcat aatctgatgt tgatgaaaga aagcgatacg ctagtgtgtc
     6661 tatcagtaag ccacccatat tgacgccatg caggttcttc caggctctga tcattttaca
     6721 cagcttgcgc agattattgg atttatctcg cgaaaatgcg gtcatggccg ccttttcgtg
     6781 aagaggtttt gtaatgcgcc atgctcctcc attatagctt tcagggaatt taaagctgtc
     6841 atcatcctgt ctgaatacag gttgaacctc gacataaaag tttttaaaga tgatctgaac
     6901 aaccagtctg tcctttttca cagtctggtc aggatattct tccgccagaa tgtttctgac
     6961 atctgtaagc aatgcagact gaccattatc cttacggtta taatattcat actgattggg
     7021 gggcataata taaagcatat ccagatcgga tatgcctttt attgcggtat gtctgcctac
     7081 tgaccccact ttcaagcggt ttgctacccg ggaactcgtt cctctgaacg caagatttaa
     7141 tgacttggtt atttttcgat agtgatagct gatctttttt gcctgtttga ggtcaagatt
     7201 caaattttca agaaattcgc tgaacaattc cctgtggctc atggtgtttc ctttggcatg
     7261 tgaaacatta gcttacatta tcattacacc ttataaaaac caagatcttg ggaggtgagc
     7321 gttctgcatg gctttatgac ttaaacgtaa gcgttaacgg agtgccgtat tgacgcttat
     7381 ttattggtga gtactacgtt ccatggcagg agttcgtcaa ctcggttgga aggccattcc
     7441 ggcagtacgc tcaggatatg gcgcagatac gcttccggat cgatatcgtt cagtcggcag
     7501 gtgccgatca gcccgtacaa cagtgctcct cgctcgccgc cgtgatcgct gccgaagaac
     7561 ataatgaatc gccacggata atctagacac ttccgagccg ttgataatac tggttttcat
     7621 attctgtcgg tgacatctgt tcgctagaac catgccgacg cttactgtta taaaacattt
     7681 cgatgtaatc aaaaatatca ctgcgggctt cttcccgcgt tccgtagatc tttttcttta
     7741 tccgttcacg tttcaacaac tggaaaaaac tttctgcaac cgcattatca tggcagttac
     7801 cgcgacggct catgctaccc tccaggccgt gtgatttcag gaacgactgc cactcatggc
     7861 ttgtgtactg actgccctga tccgaatgaa ccagcacctg tttttcggga ttacgccgcc
     7921 atacagccat cagcagtgcg ttcaggacaa tgtcctttgt catccgggat tgcatggacc
     7981 agccgataat tttgcgtgag aacagatcaa caacaacggc aagatacagc cagccttcgt
     8041 gggtcctgat gtaggttatg tccgttaccc aacgctcatc aggagcatcc ggattgaact
     8101 gtcgctggag cctgttgggt gacatgatac tggcctcgcc tttacgtgcc cgcgggcttc
     8161 ggtatccgac ctgagccttt attccgacac gtttcatcag tctccagact ctgtttactc
     8221 cgcactgttg cccgctgtca cgcagatcca gatggatttt gcgataacca tagacgcatc
     8281 ccgattccag ccagaactgt ttaatctgtc ctgtcagtct caggtctgcc tgatggcgtt
     8341 gtgaatgcgg ctgctgaagc caggcgtaaa aaccactggg atgaacatcc agcacccgac
     8401 agagcaggcg aacaggccag caacaggtgt tgtcacggat aaaggcgtac ctcagtcgga
     8461 cagctttgcg aagtacgccg ccttttttaa tatttcattc tccatttcaa tgcgttgtag
     8521 ctttttcctc agcttacgta tttcgatttg ttctggtgtt atcggagagg cttttggtgt
     8581 tttgccctga cgctcatcac gcagttgttt gacccatctt gtcattgtgg aaaggccaac
     8641 atccatagct ttggcggcat ctgccaccgt gtatttctgg tcaacaacca gttgagcgga
     8701 ttcgcgttta aactctgcgc taaaatttct ttttttcatt ggagcacctg tgttgttctg
     8761 aggtgagcat atcacctctg ttcaggtggc caaattcagt gtgccacttc aagccgggag
     8821 cggcgtcagc cagtctccct ttacccgcgc tttttcggaa agcccacccc aatgtttttc
     8881 tcctacaccc cagccggtca gcaggacctt atccccggtc tgatattcag gatgtgagct
     8941 ggcggacact gtaccggcaa aatcaatgcc tggcaccatt ggaaagtgcc ggacgatagg
     9001 cccgcggccc gtaatagcca gtgcgtcttt gtagttgata gatgaacagt cgacattgac
     9061 ctccacatcc ccctgtggca gctcgctttc agcgatttca gcgatttcag caatttgggc
     9121 actgtggcga ccatacatct tactgattaa taaggattta aacatttttt tctcgtcttt
     9181 ctggtttgat gtcattgagc tataaagtag tcaataaaca atattgaccg atcgtctaat
     9241 aatcaacgct gatatcccag tgtttgtcag aaaacaagaa ttgcttttct tttcatcagg
     9301 ttaaatctat cttttttgat ttatatcaaa ccagattcgg tcttcaatta tataggcatc
     9361 aatgttagcg gtcctacaag atacgcagtc gatgcttcag ggcgtactga tgaaataaaa
     9421 caagtaggct gagattagtt ggtctgctca tcgctcacag cggaccttca gcttcgttag
     9481 ccaagcgctg acatagttac cgatgtagcg tgttaatctg tcgggagcat agccgaaatc
     9541 acttaaccca ttagacttca cctaaaactg caaaaattac cgtctttgtc acactcaccg
     9601 agtttaatca aactaacact gtacatagtg cgaacaagag ccaaccagaa agactgttca
     9661 gcatcaacag tgaagttttt ccccgccact tccccgcaat tcttccgcag ttttcccact
     9721 agagagttcg acacatccct tgcataccaa cgctttgatt caaatctcgg tagtgcatcc
     9781 aattagtaga atatgtgttt ttcgataaat gctccgatca ctttttcgtg gatctcaact
     9841 gagcgtgaga agcagattgt ttacgagcca accgcttaat gtgggtgcgt agcgtcagat
     9901 tattacgctc aatgcgttgg gtgaatattt tgccggtcag atgcttattc ttcggcacca
     9961 cccggccata gctgtcccag tcatcgctgg tgagtatgcc gatgttgaag ggtgtaagca
    10021 gtgccagtag ctcccggcac gtttgatcgg ttcggggacc aaaagtgtag gccagtacac
    10081 cgcctgtttt ggtgttgtac gcgtaccaga gccagtgtag ccgggcttta ctgccaacgt
    10141 agctccattg ctcatcaagc tcgcagataa gcgccacatc agcatgggca acaggcgaag
    10201 aagttatttg cgttggtgcg agttttttaa agtccggatg atggtgttaa taccaatttt
    10261 cagtgtcctg gcggtatcgc gaaccccggc accattaaag gccatttcag tgatcatctc
    10321 tttcataccc ggcttacgtg cttgataagt ataagtgagc tgaaacacac ggtggcagtc
    10381 acggcagcga aatctgtcac ggcctttagg gttctgacca tggcggtaaa cctgagctga
    10441 ctgacaacgg ggacaatgaa tattaacgct ggccatgaga taacctcaaa agcccgtatt
    10501 atacatcaga ttcaagcaat tagaggcatc accctttcct acattcgtag gactttgatt
    10561 acatatagca agggttaatt cttatataca tagaataaat taggaatata atgaatttta
    10621 tcgtgacgac cttatttata attaatttat atcgtcagag aaattagtgt ccacaacatg
    10681 aagccttaca aagagattct atgatttaaa aaaaataaga gccagcttat gcatatcgat
    10741 tcgactgaaa aaaataataa acaaaagttg ggcttgcttg cgctaacatc cattgtcgtg
    10801 agctcgatga tcggcagcgg cgttgacgga ctcccccaga atatggcgga gaattctgcc
    10861 gtgggcccgg tggcgatagc atggatagtc tgtggtatcg gcatgttttt catctccaga
    10921 acttttatcg tgctgtcaaa cattagacct gatctccagt caggagttta tatgtatgcc
    10981 cgggaagggt tcggccgatt tacggcattt aacgtcgcct ggggctactg gctgatgact
    11041 atattcagca acgtcgcctt tgccattatg gtgatggata cgctgaatta tttcttacca
    11101 ggtgatttca agggagggaa caatattcct tccgttatcg gtgcctcttt gctgatatgg
    11161 ggattcaact ttttagtcct ctccggtacc cgcattgccg ggtcgattaa catcgtcggc
    11221 acgctggcca aactgatccc gctggtggcc tttattatta ttctcatgct gtttatgaag
    11281 tacaccacgt tcgcagataa cgtctggggg cacagcgcga gtaaggccag tcaggaccta
    11341 ggctctattt tctcacaggt aacttcaccg cttagcgtgg cactctggag cttcatcggc
    11401 gttgaggggg ctgtcgctct ttccggtcgg gccaaaaata aacgcgacgt gggtaaagcc
    11461 acattcattg gcttcgttgt atcactgatt atctgtctgc tggtctccat cctgccgttt
    11521 ggcgtgctga ctcaacatga gctcagcgca attccaaccc catccaccgc cggtattctg
    11581 aagattgttg tcggtgaatg ggggaagatt atgattatct tgggggtgct gatttcggtg
    11641 cttaccagct ggctggcgtg gaccatcatc tgcgccgaac tgcctatggt ggccgcgcag
    11701 aacggcacat tcccgaaacc gttcagcaaa aaaaatgtga agggttcggc ctcggtttcg
    11761 ctttatataa gcagtgcgtt tatgcaaatc gtcgtacttc tggtctactt ttctaacgat
    11821 gcctggcttg caatgctggc tatctctacc ctgaccgttt tacctgcgta tttaatgacc
    11881 acggcatacc tgtttaaact atgtattagc ggcgagtatg ccagctatac cacgaatcac
    11941 agccgcagcg ccgcgctggt cagcagtatt atcggctttg tattctgcct gtttatgttc
    12001 tacgccagtg aaatcaaata tctggcgatg gttccggtgc tgcttacgct gggcgtgccc
    12061 ctgtttatct gggcaagaaa aaaagacgat cgcgcagata aaatatttac gctacgcgaa
    12121 ctggcatttt ttattttact gctggtcctc gacatcattg tttttattct actgcattat
    12181 aaaacgatac atttttagtc cagattaatt cacagcaagt taaggtgacg ataatgtctg
    12241 tttcttgggc atgacatcct cacagttaaa tactatctga acagtaacag tggagacacc
    12301 taatgaaatt taaccacaat cttttattta tttcctcaca atatcttgat ggcgacaacc
    12361 cttcgcagca ggtactggaa gagcttcaaa ccgaactcgc ggaacgcggt tttaaaattc
    12421 atatcacgca ccagatctct gatggattaa aaatcatcga aaaaagcccc caatacagcg
    12481 ggattggttt ttactgggag ccagacaatc cgacctttgc agaagagcta cagcacttta
    12541 tcagcatatt ccgcaaaaga aatgccacga cgccgctgat aattttctca gaacagaata
    12601 ttaccgatcg tatcccactc gacgtcttaa aagaggtcag cgaatatgtt tatctgttct
    12661 ctgaatccgc agccttcact gccaatcgac tgtactcctt agtccaccgc tatgcggata
    12721 agcttcttcc tccctacttt aaaaccctta aggactttac cgaggacggt gactattact
    12781 gggactgccc ggggcatatg gggggaatgg cctatctgaa acatcctgtt ggcattgagt
    12841 tcattaactt ctttggcgaa aacatgatgc gcgctgatat tggcgtggct accgcggaga
    12901 tgggggatta tctgatccac gccggaccgc ctaaaaaatc ggaagaaatt gcggcccgac
    12961 tgtttggctc tgattggacc ttctacggtg tttccggctc atcggggtcg aaccgtattg
    13021 tggcccaggc tgcggttggc gcagatgaaa ttgccattat tgaccgaaat tgccacaagt
    13081 cacttaatca tggcctgacc ttgtctcagg caaggccggt ttacctgaaa cctacccgca
    13141 acgcgtgggg gctgattggg cctatcccga ccgggcgcct gaagaaggcc agtattgatg
    13201 cgctggtggc aaacagccga ctggcatctg gtgcggtcag ccagtccccg tcctacgccg
    13261 tggtaaccaa ctgcacctat gatggcttct gttataacgt gaacgatgta gtcagacacc
    13321 tgggtgaaag cgcaccgcgc atccattttg atgaagcatg gtatgcctat gcgcgtttcc
    13381 atccgctata ccagagtcgc tatgcaatgg acgcggagga aacgccaaat cgtccaaccc
    13441 tattcgccgt acagtccacc cataaaatgc tgccctcact ctccatggcc tcaatgattc
    13501 atgtgaagaa aagcgatcgc gcgccgctta atttcgatga ctttaacgat gcctttatga
    13561 tgcacggcac gacctcaccc tactacccga tcattgcatc aattgatgtg gcagtaagca
    13621 tgatggaggg tgaatccggt tattcgcttg tgcaggagtc aattgaagaa gcgattgcct
    13681 tccgtaaagc cgtggtcagc gtcaagcgcc agctccagga gcaggaaggt ggtgacgcct
    13741 ggttcttcga tgtgcttcag ccaaccgaag tgcaggacag cgattctggg cagcgataca
    13801 gttttgaaga ggcacccgta tcgctgctga gccacagcgc cgactgctgg tcgctgcgct
    13861 ccggtgagcg ctggcacggt tttgccgatg atgatctggt cgaaaccaac agtatgctcg
    13921 atccagtaaa agtgaccctg acttgtccag ggatcggccc gaagggagaa taccagaaaa
    13981 acggtattcc gggatatttg ctgacccgtt ttctcgatga ccgtcgcatt gaaattgccc
    14041 gtactggtga ctacaccgtg ctgatcctgt tctccgtcgg catcaccaaa ggcaaatggg
    14101 gaactctgat tgaaagcctg ctggccttta aaaagcatta cgacaatgac gatctggcca
    14161 cagatgctat cccgtcgctg aaagcgcatt ctccacacta cgacacgcta accctcaagg
    14221 agctatgtca gataatgcat gaaaaaatgg atgagctgga gctgatgtcg catatcaacg
    14281 acgcggtaaa caccgatccg gaaccagtta tgaccccggc agaagcctat cagaaagtcg
    14341 tacgctacaa aaccgagcat atccggctgg atgattttag cggcagaatt gccgccagca
    14401 tgctcgtccc atatccccca ggcattcctg tactgatgcc gggcgaacgg atgccgcaag
    14461 gtaacaaggg cattattggc tatctgcgcg ccctgcagga attcgataag caattcccag
    14521 gttttgaaca tgagatccag ggcgttaacg tcgatgaaga cggggacttc tgggtcagag
    14581 cgattgtgga agaagaacgc gatgggcagt cgctacccgg ccacataacc tttaaacgcc
    14641 aggtctctgg tattaaaaag ggccgccagt aggcggggga aagaacaata ccgtcggatc
    14701 gcctgcaatc cgacggcaat attatagaat agctgaggga aacgttatgg cagagcaatc
    14761 ttcagatgta aaaaatagtg acaatagcgc cggaggacat aagctggggc ttatatccct
    14821 catcgctatt gtcgttagct ccatgctggg aggcggcgtt ttcagtctgc cgcaaaacac
    14881 ggcggcctct tctgcactag ggccagtaat cattgcatgg attattgccg ggatcggtat
    14941 ttattttatc gcaaactcct ttcgcgtact atcagatctg cgcccggacc ttcaggctgg
    15001 agtgtatatg tatgcccagg aaggttttgg ttcctttatt ggctttaacg tcgcctgggg
    15061 ctactggctg atgaccgcat ttggcaacgt tgcctttgcg gtaattttaa tggatgcttt
    15121 taatcagttt atgccggggg tttttacgga tggtaataat ctgaactcca ttatctgcgg
    15181 ctcagtgctg atctggggct ataattttct tgttctgtcc gggacgaaag tggccggatt
    15241 tgttaacacc ttgggaacca tcgccaagct cgtccctctg atcctattcg tattactgct
    15301 gggtgtgctg atcgattatt cggacctgtt taaaaacttt tggggagaat cacccgcgat
    15361 cctcagtggc aacggaaatg acgccgcacc agtatcgctg ctgtctcaga ttctggcacc
    15421 gatgaccgta acgctctggg cctttattgg cgtcgaggga gcggttgttc tatccggacg
    15481 tgccaaaaat aaacaggacg tagggaaagc cacactgatc ggctttctgc tggcgctaat
    15541 tatttatatg ttaatgtcca ttctgccgtt cggtgtaatg cctcaggcag aactagcgca
    15601 ggtcagtaac ccctctaccg caggcgtgct cgcccacgtt gtcggtcagt ggggcgactg
    15661 gctgatgagc gcggggctga ttatttctgt tctggccggc tggcttgcct ggaccatgct
    15721 atgcgctgaa ataccgatgg tagcaggaca gaaaggcgct ttcccgcgcg cgtttgcccg
    15781 aacgaacagt aaacaggcgg ccaatgtgtc actgtgggtt agtagctttg tgatgcaggc
    15841 ggccatgtta cttgtttatt tttcaaataa tgcttggacc accatgtata atatcagtgc
    15901 gctgatggtt gttccggcct atattaccac cacgctgtat ctgaccagta tttgcagaaa
    15961 cggaaaatat gatcagtatg caaaaaaagg ccgcaggctg gctttgtcca gcggtgtttt
    16021 aggctcgcta ttctgtctgt ttattcttta cgccagccag attgagtatg ttgcattaat
    16081 tcctatcctg ttgacctgtg gtctgccggt ctttatctgg tcaagaaaag aaaaaaatga
    16141 tggtcagccg atatttacca aaaaagagct gatttatctg attatgctgt tgctgtgcga
    16201 tattatcgtc gttgccctgt ttattggtgg atatttaaag gtataagtcc gagttaaatc
    16261 tttactgggc tagtaatgcc gccttttaaa caagaagggc ggcaatctta ctgaaattag
    16321 tatgctgcaa atcggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag
    16381 gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg
    16441 tgcgtaacgg caaaagcact gccggacatc agcgctatct ctgctctcat tgccgtaaaa
    16501 catggcaact acagttcact tacaccgcct ctcagcccgg taagcaccag aaaatcattg
    16561 atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg cattatgggc gttggcctca
    16621 acacggtttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacaacc
    16681 gggcagtgat gtgattgtct gcgctgaaat ggacgaacag tggggctacg tcggtgctaa
    16741 atcacgtcag cgctggctgt tttacgcgta tgacaggata cggaggacgg ttgtggcgca
    16801 cgtcttcggt gaacgcactc tggccacact ggagcgtctt ctgagcctgc tgtcggcctt
    16861 tgaggtcgtg gtatgggacg gatggctggc cgctgtatga atcacgcctg aagggaaagc
    16921 tgcacgttat cagcaagcgt tacactcagc gcattgagcg acataatctg agacaacatc
    16981 tggcaaggct gggacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg
    17041 tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt acctggtttt
    17101 tgatgccagc cataacgtgt ttgactacaa agtattacga aagaacttta atgagcccct
    17161 ggaataattc attaaagtaa tttacttcgc tatccttttt ccagttactc acaaagaaaa
    17221 atattcagaa aataattccc gggctggctg cccgggatga ttactatttt aatggattgt
    17281 taatctttgc atattcaaac gggctgatac attgttctct gttcgcatcc agaaaatctg
    17341 cccaccactg tagcataagt cgacgttctt ccagatgttc ggctttatgg atatacgcag
    17401 cacgtacacc attacgctcc tgatggctca tctggcgttc cacagcatcg cgggaccaca
    17461 aacctgattc tattaatgca ctacatgcca tcgcccggaa tccatgacca cagacatcct
    17521 gggttgtgtc ataccccata accctcaggg ctttatttac ggtgttttca ctgattggtt
    17581 tacgtgggtc
//
LOCUS       DAZFRE010000099        16980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFRE010000099 DAZFRE010000000
VERSION     DAZFRE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.099"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(418..954)
                     /gene="yghD"
                     /locus_tag="ACHKUI_003947"
     CDS             complement(418..954)
                     /gene="yghD"
                     /locus_tag="ACHKUI_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO6600042.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAA
                     VFLFCAVYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIIAARKSGTTTQAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVLRLEFGRG"
     gene            complement(956..2134)
                     /gene="gspL"
                     /locus_tag="ACHKUI_003948"
     CDS             complement(956..2134)
                     /gene="gspL"
                     /locus_tag="ACHKUI_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6600043.1"
                     /translation="MSSILEIFFPLCDSDTVRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPFSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(2131..3108)
                     /gene="gspK"
                     /locus_tag="ACHKUI_003949"
     CDS             complement(2131..3108)
                     /gene="gspK"
                     /locus_tag="ACHKUI_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6600044.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHQAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(3105..3710)
                     /gene="gspJ"
                     /locus_tag="ACHKUI_003950"
     CDS             complement(3105..3710)
                     /gene="gspJ"
                     /locus_tag="ACHKUI_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6600045.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMALQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3707..4078)
                     /gene="gspI"
                     /locus_tag="ACHKUI_003951"
     CDS             complement(3707..4078)
                     /gene="gspI"
                     /locus_tag="ACHKUI_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6600046.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(4075..4638)
                     /gene="gspH"
                     /locus_tag="ACHKUI_003952"
     CDS             complement(4075..4638)
                     /gene="gspH"
                     /locus_tag="ACHKUI_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6600047.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(4642..5097)
                     /gene="gspG"
                     /locus_tag="ACHKUI_003953"
     CDS             complement(4642..5097)
                     /gene="gspG"
                     /locus_tag="ACHKUI_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6600048.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(5114..6337)
                     /gene="gspF"
                     /locus_tag="ACHKUI_003954"
     CDS             complement(5114..6337)
                     /gene="gspF"
                     /locus_tag="ACHKUI_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6600049.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQHLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6337..7830)
                     /gene="gspE"
                     /locus_tag="ACHKUI_003955"
     CDS             complement(6337..7830)
                     /gene="gspE"
                     /locus_tag="ACHKUI_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6600050.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7830..9890)
                     /gene="gspD"
                     /locus_tag="ACHKUI_003956"
     CDS             complement(7830..9890)
                     /gene="gspD"
                     /locus_tag="ACHKUI_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6600051.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLALVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9920..10879)
                     /gene="gspC"
                     /locus_tag="ACHKUI_003957"
     CDS             complement(9920..10879)
                     /gene="gspC"
                     /locus_tag="ACHKUI_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6600052.1"
                     /translation="MARVVFRDARIYLIQLLTKIRHTLSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTKAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10897..11307)
                     /gene="gspS2"
                     /locus_tag="ACHKUI_003958"
     CDS             complement(10897..11307)
                     /gene="gspS2"
                     /locus_tag="ACHKUI_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO6600053.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11373..12182)
                     /gene="pppA"
                     /locus_tag="ACHKUI_003959"
     CDS             complement(11373..12182)
                     /gene="pppA"
                     /locus_tag="ACHKUI_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO6600054.1"
                     /translation="MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12312..16868)
                     /gene="sslE"
                     /locus_tag="ACHKUI_003960"
     CDS             complement(12312..16868)
                     /gene="sslE"
                     /locus_tag="ACHKUI_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO6600055.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTG
                     ATCNGESSDGFTFTPGEDVTCVAGNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNGEA
                     LSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGCNSQRWFSLTARNVNEGQIQGV
                     INKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPH
                     YNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASMTV
                     GTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYV
                     TQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTG
                     EVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIFE
                     KFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNVSVWLWNKIEYRYENDKDDELGF
                     KTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESSASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVTFK
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNFTGGVAEFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKQWYPEGDLPEFYSDREGMKGWNLFQLMHRKARGDDVGKTKFGERNYCAES
                     NGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMNFEGGVSQSAYETLAA
                     LNLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         3777 a         4826 c         4241 g         4136 t
ORIGIN      
        1 agcagacatt tcttaaaatc gacgaatagt ttcactggaa attattatgc ttatttgaaa
       61 ttccctttct gattatctcc aggaatttta ctaatttcca agcaacacaa aatacagatc
      121 acacaagaat tatcctatat gttttgacct atcaattgtt actcaatgat ttattacaac
      181 tattaataat cataatgtgt caaaacactt aagcatcttt acagtcttag aaaggtgtcg
      241 attaacgaag gctacatggt gaaaaacagg ttccttcagt gttaccgaac attccgtggt
      301 ggtctggagg gagataaatc ttgctacatc ggaactcagc ccggtgagct ggtttgacag
      361 ttccgcactc accattgggc taagggttat caggtggggt taaggaaatg gcaataccta
      421 cccccgtcca aactccagtc gcagcacatt taccatccct ggcttctcag cagcactgac
      481 atcaatttgt gtcacccgca gcgcatattt ttcatccagt gcgtttaacc atttcagaag
      541 gtcattaaac accacaggtt ctatccagac ctgaatattc tccccacgct cggctatccg
      601 cttgatgacc accgagtgcg cggaagcgct gtcactgatg acccgcgata cctgtgcttg
      661 cgttgtcgtg ccggattttc gcgccgcaat aatatccggc gcagtgctct tcagtcgcgc
      721 gttcatcgcc accagctgct gcaacatcgt ctcctgttgt tctatccgct cgctcaacgg
      781 ctgccagatg aggacgtaat atacggcgca aaacaggaac acggccgcgg ccagtaacat
      841 gcctttttca cgcggcgaac gccccgccag gtgctgtgcc agccagtgtt cgccacggct
      901 taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt
      961 tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt
     1021 gcacaacata atctgccgcc agtgcgctac gcagtttatc gaagctggca aagttcgcag
     1081 cccgtagctg gaggtgaagc gtctggcgtt tttgatcaaa ggtgaaacca cgcatttcga
     1141 tgtcggtaag tgacgctgat ttcagggtac tggcgatcgc tgacaattct gcgagcagcc
     1201 gggtatcgtc ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat
     1261 tcacaatccg cttctgctcc gggaatagcg ttaagaactg tttctccgcc tgggtgcggc
     1321 tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgttccact gccagcgcaa
     1381 ccagaatcag caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc
     1441 tgacgcgagg ctgccacggt ccagtcagca ggttcccttc cggttcgcca taagtggtaa
     1501 tggcgggcag agccgtcact gtcaggcgtt cggcgtctgc accagcccat gcagacagtt
     1561 cttccggtgc aatgccgacc acggttagtg aaaacggtaa atcctgctca ttgagctgtg
     1621 cgcggaacat gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcgatgc
     1681 ggcagataac ccgttgcgca tcgccagcca tatagccaca aggaatggac atccagtctg
     1741 gcgcgacggt agcgcgggtg atgccgtttg cctgcaacca ttgcgcaatg ttacgcatat
     1801 gctgttggtg aatcactgct acggttgcca gttgctggtc gattttcaac ggggcgaaat
     1861 gcagttcatc gatatcctga ttcagctctt cttccagcaa ggcgggcaga atagtcggta
     1921 tctgcttgcg ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa
     1981 tgtagagtcg aatcgcatcg gtttgcagcc attgctggag acgttcgtca gcaacgtcag
     2041 gccagatacc gtgttccaca tcgggtgtac ggcgctgcca gcggacggtg tcggagtcgc
     2101 aaagcgggaa aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag
     2161 aacagaaaag tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc
     2221 attcacggtg atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt
     2281 tagctgtttt ttagtacgct catcgacgtc tgcgagtagc ggctgtgcaa gaaactgatc
     2341 gacatcttcc cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gtacagggct
     2401 taaccacggg tcaaacagcg cctcaagaat cacactttgc gtgacatcca gggtgttgat
     2461 gttgatttgc tggcgggtca tcggcagcgc acagaccagc ggtttcagtt tttgataaag
     2521 tccggcgtcc attccctgca ccacgcgcat ctcactgata tcggccagcg gttgattagc
     2581 ggcgtaaaac ggcaccgaac gggcgagata ctcgctgtct tcacgaccca gacgcgtctg
     2641 cacgctgcgg tcttcgtcaa taaactccca caggctttcg gctatcagtt cggcacgata
     2701 agcaggcaca tcgaggcgcg agatcagggc aatcagttgt tgtaccgcga gcggacgcga
     2761 cgccgtcgtc ggctgagcga gggcattcag gttaaagcag gcctgtgcgt cacgcagagt
     2821 gacggcgatt tgcccctgcg gcagtgggaa aaaacgaggg ccagaagccc acggctgtgc
     2881 ctgatgcaca cgcttttcat ttttcaggct cagactgagt gcgctcaacg ccaggctttc
     2941 cgcactggcg ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag
     3001 ttgttgctgc atccgcccgg aaagcgtgat ggtcaccagc atcataaccg ccagcaatac
     3061 cagcaccacg accagtgcca ttccgcgttt tggtggtgag gtgatcatga taattgcggc
     3121 ccgcgtaaca accagatgcg ttcaatttcg ccccattgcg gcgaatgcag ggttatgcgt
     3181 actgccacag gaatcgcctg cactgatgac cagctctcct gccagcgcgt gccgtcgtag
     3241 aactgcaaac ggagcgaatc cgccgggatt aatttttgcg ttgttggctt cacgctgcct
     3301 gccgcatcgg tcagtggcca ggctaaccgt tcgagataac caccatgaat gcggtaaccg
     3361 acggtgagca gattactgcg cggcagacgc atcaacggat taaccacgcc gccgcgcaca
     3421 aaacgcatcc cttcactctc agacgccagc acgccagcgc ccgccagtaa cgctggttca
     3481 cgctggccct gatcgcctct taccggacgc ggcatcattt gtgtcagatc gtggttcaaa
     3541 aaactcatcg tttgctgcat gaggtttagt ttttgatcgt gtccggcgac ggcgctattc
     3601 acgcgtgtaa cgccgtttgt cacctgcagc gccatcagtg ccagtgaggc aaaaatggca
     3661 attgccacca gcatttccag taacgtgaaa ccagcgcaag tccttctcac tgttggcctc
     3721 ccacggcgct aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc
     3781 tgacttcaat atccactgcc tggagcagcg cgttgcctgt actcagcggt gtggttcgcc
     3841 agtaccagcg gcttcctgcc atatcactct cgccctggtg cctgaccgct cgttgttcac
     3901 gggtcatcag gtaaagcagt gcggtctggt tttcagctac ccagcccgct accgtttttt
     3961 cttcaaggat ttgctgatta ctcagcgcgc cgctggcgat ctgtaacacc gccatggcgg
     4021 ccaacgcaaa aatcgccagc gcgagcatta cttccagcaa ggtaaatcca cgcttcatgg
     4081 catcccctca tcacattgat tgagggataa cacgccatcg tgcgccagct ttaccgccca
     4141 acacgcgttt tgtgccgacg cgtaaaaacg cagtgtaaac ggcgtggcgg gttcaaaagg
     4201 cgaaaaacgg atctgcggtg tcttcttttt cgcctctttt tgcagctcca gttcaatatc
     4261 gtgcagcgtc aggcgacgac gctgcagctc cagcgcatat tccttctgcc agatatcact
     4321 tccgggttgt aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt
     4381 cgcagaaacg ggcaaccatt gcccgtgacg acgctgcata aactggtaac ctggcgggtc
     4441 gattagcaca ccgagtgttt gcccttcgat cactgccctg tccttaaact gcgcaaagcg
     4501 agtcagaaaa tcctgcgccg cttttttcgc aggcggctct gaagcggtcg caaacgtctg
     4561 taccacgccc gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt
     4621 gaatccgcgt tcaggcactg attactgaaa ctcctgcaaa ttccagttac cgatatctgc
     4681 gccagcgccc tccccgtttt cctgaccatc tgctcccagg gtataaacat caaacagccc
     4741 tttttcaccc gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt
     4801 aatgtatcca ccggtacggt agttacggga atccgccata ttggccggtt gctggatcag
     4861 cgcctccagc ccctgttcgg tagtcggata acgcccgtta tccagtcggt acatatccag
     4921 cgcattctcc agcgccacga tatcgctgat ggctttttgc cgatcggctt tctctttgtt
     4981 gcccaacagg ttaggcacca ccagacttgc taggacgcca agaataacaa tcaccaccat
     5041 cacttccagc agggtaaaac ctgcccgtgg tttttgtgtg cgggataacg aattcatgtg
     5101 ataactccgt aaattacatt ccaaccatat tgttcagttg cagcatcggt tcgaggatgg
     5161 cgatgacgat aaacagcacc acgcccgcca tcatcaccac cagcgccggc tcaaacagcc
     5221 ctaacgccag ccccacctgg gtatcaaatt cccgttcctg gttgaccgcg gcctgttcaa
     5281 gcatggtttc cagttcaccg ctctgttcgc cggaggcgat catgtacagc atcatcggcg
     5341 ggaacaggcg caactccgcc agcgcggcgc gcaggctgct tccttcgcgg acgcgatctg
     5401 ccgccagcag cagttgttgc tcgacataac gatttgccga cacggcggcg gcggtctgaa
     5461 tgccttccag cagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg
     5521 ccgtattcag tccgcgcgca acacgccccg tcacgggcaa acgcagcaag gttttatccc
     5581 agcgcaggcg catcgcagga tttttgagta gccgttgccc cagcaccaga agccccagca
     5641 aacccgccag ccagtacacg ccgctggtct gtaacgtgtc gctcatggcg atgagcattc
     5701 gggtggaggc aggcagcgcg tgtccgagat gatcaaactg ttcgataatt ttcggcacta
     5761 ctgccgtcag caaaatagtg accacgcccg ttgccaccac cagcagtacc agcgggtaga
     5821 gcatggcctg cagcaggcgt gatttcagat gctgccgctg ttcggtgtaa tccgccaggc
     5881 gattgagcac cacgtcgaga tgtccggatt tttctccggc agcgaccatc gaacaaaaca
     5941 gggagtcaaa gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa
     6001 tccggctgcg cagcgccatt ccgaggcttt ttacatgcag tttttcactt tgctcactga
     6061 ccgcctgtaa gcaggtttcc agcggcattg ctgcctgcac cagcgttgcc agttggcgtg
     6121 tgaacagcgc caaatctgcc gccgccacgc gacgatgtgc atgccgccga cgctgcaaca
     6181 ttccccctgt cgaagcattc agccgggctt caatgtgcac ggggataagc tctttaccac
     6241 gcaacaattg acgggcatga cgcgcggaat ccgcctcaat cattcctttg gttttgcgac
     6301 cattacgctc cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag
     6361 aacttcatcg agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc
     6421 cgcagagtgt tgccggacat aacgttccag ctccagttcc ccggcctgac ggtggatcaa
     6481 atcacgtaat gtggcgtcca ccacgatcag ctcatggatg gcagtacgtc cgcgaaaacc
     6541 tttgtgatta caggcgggac agccctgcgg atggtacagc gtgacggttc gcgcatcggt
     6601 aatccccagc aggcgttttt cttcgtcggt ggcaggcgcg gactggcggc aatcgggaca
     6661 cagcgtgcga accagtcgct gcgccatcac gcccgtcaga ctggaagaga gcaggaaagg
     6721 ctcaacgccc atatcctgca aacgcgtgat cgcccccacc gccgtgttgg tatgcagcgt
     6781 ggaaagtacc aggtgtccgg tcagtgaagc ctgaacagcg atttctgcgg tttcggtatc
     6841 gcggatttca ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc gcaggccacg
     6901 ggcgaaggtc atgccgacgc gggtgttaac ctgcgtctga ccgatccctt caatcatgta
     6961 ttcgataggg tcttcaaccg tgagaatgtt acgcgagtgg ttgttcagct cctgcaatcc
     7021 agcgtacagc gtggtgcttt tgccggaacc ggtcggcccc gtcaccagaa aaatgccgtg
     7081 cggtttgtgt aacagctggc gcaactgcgc agtcagttcg agacttaaac ccagacgctc
     7141 cagcgtcagg cgagcctggt ttttgtccag cagtcgcagc accacccgtt ccccccaggc
     7201 ggaaggcatg gtggagacac gcacgtcaat cgcccgaccg cccagcagca gcgcaatgcg
     7261 cccatcctgc ggtacgcgtt tttcggcgat atccagccgt gccatcacct taatacgcga
     7321 taccagcagc gaggccagtt tgcgccccgg acgcagcatt tcatgtagtg tgccgtcaac
     7381 acgaaaacgg atcaccagac tcttttcaaa cgtctcgatg tggatatccg aagcgccttc
     7441 tttaatcgcc tctgccaaca tggcgttgat cagtttgatg attggcgcat cgtcgtcact
     7501 ttccagcaga tcttccgttt ccggtagttc ttctgcgaga gtaaaaaagt cctcagcaga
     7561 accgagatct tccatcagct ggcgggcctc ggaagagtcc cgctgccaga ccgcattcag
     7621 ccgctgttca aattcggcct cgtcgatttg cctcagcgta aaagacgcgt tcagcccccg
     7681 ctgcagctcc tgcaaaacag agagcgacaa cggatgaaca tggaggatct ccagcgacgc
     7741 ttcgcaccat gccaccaggc taaaccgacg gctgaaactg tagggcagac gcacggtgtt
     7801 agcggtggtt tcctgtgcta caggcaccat taacgcgttc tcccggcatt aaggaacgcg
     7861 cgaacttctg gcggtagagc ctgattttgc gctggcagta ccggctgcgc ggtgtgcggc
     7921 atcaggctca agccttgctc atcgcgatag atctgttcgg cgcgcatata gttatatttg
     7981 cgctgcgaca cgccgtctgc cgccataccg tcacgcagaa tggtcgggcg gataaatacc
     8041 atcaggttac gtttttcttt tttatccgcc gtcgatttaa acaggttacc aatcaatggg
     8101 atatcgccaa gcagcggcac tttcgccacg ctttctcccg cctgatcgtc catcagaccg
     8161 ccaagcacga tcagctcacc atcgtttgcc agcacggtgg ttttcagttt gcgctcgcca
     8221 aacacgacgt cgagactggt ctgtccttcc accttcgaca cttcctgctc aatcaccatc
     8281 tgtaccgcgt ttccttcgtt aatctgcggc gtgactttca gcatgatgcc gacttttttc
     8341 ctctctaccg tattgaaagg attgctgtta ttagagccaa cagtagagcc ggttaatacc
     8401 ggaacgtcct ggcccaccat gaagaaggct tcctggttgt ccagcgtggt gatactcggc
     8461 gtggagagca cgttcgagct ggagtcgttt ttgaccgcct gtaccagcgc catccagtcg
     8521 cctttcacca cgccaaccgc cgtaccgcta aagccagaaa gaagctgagc gagcgtggag
     8581 agatcgccgt tagtatccgg gttaatggtg gtagcgccgt tttcactgat caccgtggag
     8641 cctttctgcg gttttgcctg agaaatggct gcacccagcg tgccaatagg gatctgcgta
     8701 ccgttagcaa actgcattaa tccggcatct ttcgacgccc actgcacgcc gaaattgata
     8761 ttgctacctt cggcaacttc cacgatcaac gcctcgacgt gcacctgagc acggcgaata
     8821 tccagttgtt caatcacgct ttgcagcgac tgcatgatgt cctgcggcgc ggtaacaatc
     8881 agggcattac tgtgtttgct ggcggcgatg gagacaacct cacgcccgct accgacggtg
     8941 ccttccgcct cttctttagc cgccgtgagc gtgccgctga cctgcttcag tacatcgacc
     9001 agatcttcgg ctttgctgta tttgagatag aaaacctggc tgttgccgct gcgttccatt
     9061 tctgagtcca gccgacggat caggcggcgc attttgtccc gcgtggccgg gtcaccactg
     9121 acaatcacac tgttggtgcg ttcgtcggcg acaatttgag atttcagcgt cgcaggctgg
     9181 ttctcgccgc tgtttttcgt caggctttcc agcacgcggg cgatttccga agccgaggcg
     9241 ttatccagcg ggatcacctc ttcggtgcga ttacccgcat gatccacgcg ctggatcact
     9301 tccgtcaggc gctccacaac tgaggcgcgt ccggtaagca taatcacgtt ggagggatcg
     9361 taattaacaa cgttgcctga gcctgcgctg tcgatcatct ggcgcagaat cggtgccagc
     9421 tcgcgtaccg acacattacg taccggcacg actttggtga ccatttcatc gcccgcgtag
     9481 ttgtcgctgc cttcaccaac cagcggcagc ggctcgactt tcgcggcact ggatttcacc
     9541 accttcagca cgtcgttttc catcggtacg acggcatacc cctgtgcttc cagcaggtta
     9601 aggaatagct ggtaatactg gcgttcattg agcggggtca tggtgcgaat actcactttc
     9661 ccctgaacgc ccggccccat aatgatggtt ttattaaggt tagcgccgac ggtttcgatg
     9721 aacgatttca ggtcggtatc tttaaaatta gcggtgaaag tggcttcttc cgcccagacc
     9781 ggtgaactgc ataatgccgc tgccagcacc agcgccagta aacgcttttt tgttttgagg
     9841 ccagttgtct tcttacgcca gaccgacaac gtcatatcac gccaaaacac gatgaatgat
     9901 tctcctggat taaatgcggt tagcgcagcg cgatggaaat gtcgtagcgc gcacccttgc
     9961 gtaaaaccgt aagttgaatg gaatccattg aaggtaactg ccgcatcaga gcaatcattg
    10021 ctcgtggatc agtgaaatcc tgctgattta gcgcaatggc gatatcgcct tccttaaaac
    10081 cgctggcatc gaacagagaa cgatctgccc ccggtttcac tgcataaccg acaatccctt
    10141 ccttacgcac aggcgtaagc tggatatagt taaaaatttt ctgcggatct ttcgccagtg
    10201 cctgacgcac ggcagccggg atctcaactg gcgcactggc agcaggctca gcaacagctt
    10261 gctttgcttc gtcgctgacg gcttttttgc tggtcacggc tttggtggga cgcttctctt
    10321 ctgccagact cagacgttcc attttcccct gatagcgcag catcacatgg tcacgattaa
    10381 tttcctcaat caccgcgttg tgagagccaa gccgttcgcc ctgtaaatag acctgctgtt
    10441 tgccgccttc ttcaatcacc gcgccgggtc tggcaccaaa ggcgatcccg cgcagcacca
    10501 cattaagacg cgtttctgcc acaggcgcag gttcgggttg ttttaccggt gcggcgacgg
    10561 gttgatattt gccaaaccag ttttgttggc tgactaattg cacgtcattt ttatcgaacg
    10621 gttttgcatc cgcacggagc ggtttattcg ccgatgagga aaccgccgta tattccgcag
    10681 aaaaggagaa atagcgccag agtgaatacg ccatttttgc agaaataata agcagcatga
    10741 gccagaacat cccgcgcacc atttttcgca gatgctcttt gtctgtatta agagattgtc
    10801 tctggctaag agtgtgacga atttttgtca gcaactgaat gagataaatt cgtgcgtcac
    10861 gaaaaacaac ccgcgccaat ttatatctac ccgacgttat gctttgacta ttccacaggt
    10921 ggtacgatcc agtttccgct gatactggtt tcctgtacgt gtatcattaa tcgttatgct
    10981 gtaattaatt ccctcggtaa tcattaattt caccgtcggg tcagcgcaca tttgtcgttg
    11041 atagctggtt aaaaaggcat caggtgtctg cgtggtctga gcacccgctt cgctgataat
    11101 ggtcattttt accgtcgtgc cgctactttg cgccagcact aaggtatatc ccgcagattt
    11161 aatcggcagg ttttggctga tattttgcgc ctgttttttc gccagtaaac tggcattttc
    11221 attatggccg gcacagccac ttaataatcc tgtaacgctc aacaatagcg atattaatac
    11281 cctccctggc atttgtttta tcgacatggt aaataatctc taaggttatt aataagagtt
    11341 aaaatgtcac tttgataatg acgtggttat cattaaaaca atgcctgtag ataaagtgtt
    11401 gctataccgc ccagacttaa acacggtcca aaaggcagtg tggttgatcc tctttttgta
    11461 ataacggcat atatcaggcc gcagcatgat gcgattaaag caacattggg tagcgacaac
    11521 ggccccaccc agccacctaa cgcagcgaaa agtaatacat cgcccatgcc taatgcttct
    11581 ttacgcagaa ctattccggc tatccagcgc agggagtaaa aagtgataaa ccccaccagg
    11641 acgccggtaa ctgcgtcttg tagcgttaac ggactctgtt gcgcccatgc cgcaatcagc
    11701 ccggtccaca atacgccctg agtaaaaaca tcgggcagcc attggttatc gaggtcaatg
    11761 acgctcgcgg caatcagcca ggcggataat atcatcaccg ccagccccca tccactttct
    11821 ggccacacca gactcgccag caaaaaagcg agtgccgtca ataactccac cagcggataa
    11881 cgcttgctga ttttcgcctg acagtcgcgg cagcgccctt tgagcatcaa ccatgagagc
    11941 agcggaatat tgtcacgaac gcggatggtc tgctggcaat gcggacagtg cgagcgtggc
    12001 agcgccaggt ttatttttga ctgcgtactc ggcatttcac cgtgaaactc cgccatttgt
    12061 tggcgcagca tgatggggta acgccaaatc accacattca aaaaactgcc gatgatcaat
    12121 cctccgacgg ttgccagtat gggcatcccc gcggagtatt gctgaaaaac atcaaaaagc
    12181 atggttaaag gttgtttgtt gtaacttgcc ggatgcgggc ctgcgggtgt atgccatatg
    12241 gctttcctcc aggcccgatg cgccttattt catgccggat gcggcgcgag cgccttatcc
    12301 ggcatacagg cttactcagc tgacatctta tgctcggtaa cctgattaat ggtttccggc
    12361 ccttgctgcg gtttcggcag attaagcgcc gccagcgtct cgtaagccga ctggctcaca
    12421 ccgccctcga agttcatctc gctcgctccc ggtaactggt aagcattcgc gcccggattc
    12481 catttcttaa agaactccga aagatccgtc tgagcgaccc aggaggcaca cagcatcagc
    12541 ttgtcggcag cgttaccgtt ggattcggca cagtaatttc tttcgccaaa cttggttttg
    12601 ccaacatcat cgccgcgtgc tttacggtgc atcaactgga acaggttcca gcctttcatc
    12661 ccttcacgat cgctgtagaa ctcaggcagg tcaccttctg gataccactg tttgatatca
    12721 aagtttttct ctgcccactc cttcagctgc gcgtacatca gcagacggtc gcccgcaccg
    12781 ccgcgcgccc atgcctgacc gttgctctcc tccagatatt ccggcgcgac agtaatatcg
    12841 tcagcgacac ggttcatctt gccgagatag cgatcctgca tgtacagcgc cagaacgttg
    12901 ttcgctactt cagttgcgcc aggaacagtc agcggcgttt cggcggcgtt gtgccctact
    12961 tcgtgccaga tcaaccagtc gttcagcggc gtcgtcggca gcgtggtgct gttcgtcgag
    13021 aagctgctgt tcattaccgg ataacccgag tgcgcatcac cgatggagat ctgcacatcg
    13081 ttggtgaaac gatgcttgtg ccccgtcaag tttttatagg taaacatccg gtgcttaccg
    13141 tcttcatcat tacgaccgta gaagtcattc atcgagctgg caaaggtatc cagatcttta
    13201 gcgaattctg ctacaccacc agtgaaattg ctggcctcaa ggttcttctt cggcgtggta
    13261 taaacgaaag cgtctgattc cagctcgccc agcggcgcag gggagttcag agcgtttttc
    13321 cattcgccgt ctttatagaa cggcgctttt accacaccag taaaggtgaa ttcggctgac
    13381 tcattctttg ggctgttgcc cttgatataa atcagaccac cgtaaggcac cttgaaggtc
    13441 accttatcat tggctttcag atcataggtt ttcgtcactt ttggcggacg gttcagcgcg
    13501 acttcatgct tctcgcgtcc ggtcagatcg tcggccagcg ccacggtgac agtcacagga
    13561 actgatgcag aagactcaat ggtgacctct ttctgagccg gagcccacag gccagtagac
    13621 tgcatgttac ccgcaaacca tttggtcgga ttggagtaca ggctgatggt ttcagtaacc
    13681 ttctcacctt ctgccgatac cgctcccgga tacttctcga catcaacttt gatgttcaga
    13741 tcccaccagg aacgacccag catcaggcgc gtcagcggtt tttccatata gttgagcgga
    13801 tagctcgggt tcatcatgcc cgctttatta acgctcttct cgccgtagat catgttgtta
    13861 tcgaccagcg attttttcag ctcatcagaa cactgcgtgc cgccagtata ggcatcgttg
    13921 gcgtagcagt tcaggaactc ggtgaacgtt ttaaagccca gctcgtcatc cttgtcgttt
    13981 tcatagcgat attcaatttt attccacagc cagaccgaca cgttctggta cagacgttcc
    14041 agatcgacgc tgctcagacg ctcacctttg cgaccattgg tccggaagta gagctcatgc
    14101 tgatacagac gctgaatgtt ggtgcctaaa tccgcagcct gcaccatcgc ttttgccgtg
    14161 tcggcgttaa ggcttagttg cgtatactgt ggaacataca tgccaccagt aaccggaacc
    14221 cccgtgccag gacgatattc cagacagttg acctcgtagt ggtaagttgg gtccttacac
    14281 tcctttaatc caggaaactt ctcaaagatt tttgccttcg cagccttcag agaatcctct
    14341 gttttatgat cggcctcatc aataaaggca taacgcgttt cctgtttgcc atctacatct
    14401 tccagccagc tggcaacttc cagcttcggt ttgtcatcag gtttgttttc tacctgatat
    14461 ttccacttaa cttcccctgt cttactatcg atggtgtacg gcagcgcacc atctacggca
    14521 ggataacgtt catagaccca aatgcccgtt gcgcgctgct gacgaacgcg gttcggatac
    14581 ccttgcggat cgttatttac taccgacttg ttcagtgcca tcgacagacc tgcggcatca
    14641 agcagacgta caaagccaga cgcgctctct tccttaagat tgctcatcac gttttccatg
    14701 atcagcaccg atccaccttt gttcagatag gcgatcagat cggtcacatc ctgctgagtc
    14761 agcttcggtt tgctggtatc tgctcgcagc gggattgcat aaggatcgtt accaacctga
    14821 gtcacatact caaagccgtt gaggatcagc agcggcattt cctgcgggtc gagatcgcca
    14881 tagctactta aatgctcaac agagataccc gcaaaatccg gatgaaagcc gaactcagcg
    14941 ctgtttcctg taacctgacc atgacgtttg aaatagacag tatccaggtt ggtgcctacg
    15001 gtcatgctgg ctttcgcgtc cggcgtccat ttatcgtcgg acagatagcg caacacgttc
    15061 tgcataaagt gcttcatatc attagaatca ctgttacgcg tacactgacc gtttttatca
    15121 acaccgcctt cccagctgta accgttcgga caacgcaaaa tgctgttgta gtgcgggtta
    15181 ccgataacca tcagtttgcc ctcaccgact tgccccagcg aaataaacgg caggttaaag
    15241 gtggcggtat cgcgcgtaac gttttccggc tcaacgagag aaggcgcttc cgtaatgtac
    15301 gccagctcgt ttttatccca ggcgcgtttt tcgccaaacg ccagccagta gtttttatca
    15361 ttacgcgcca tcagaatcgg gaaggccgcg ttggagatat tcaccactgc ctgaccgcgc
    15421 gcattaccgg tactgccata gaagttggta gagtcatgga agacgtggaa tttcgtcact
    15481 gatttgtaat ctttatccac accccacagc ttgttgataa cgccctgaat ctgaccttcg
    15541 ttaacattgc gtgctgtcaa cgagaaccaa cgctgagagt tgcagccccc aagggagtca
    15601 caaatcgccg tatcaatctc tttagcctgc ccggattcaa actgctcaag aaattcgttt
    15661 gtccgctcaa aggtttgatc gccttcgctc aacgcctcgc cattggataa cgagagattg
    15721 ataatctcgt tgattacgtt gggatactcg gcaaagacct tgcgcactac gtccggcact
    15781 tcacgctcat cattttttcc ggcctgggag taacgatgaa taagctgatc aatattcgcg
    15841 ccgcgaactt cgtcacccag ttcagtcaac gcaatggtcg acttattgcc gcgcactgag
    15901 cccagttcaa aggtgtcgat accaaaggag atggtttcgc cccagctgaa gttgaatttg
    15961 ccgttttcgc cagttacgcc acggcctgag ctggtgtagt aatttacgcc aaccacacca
    16021 ttgcccatgc tatctaccag tcggccttcg gagagaatga tttcagtggg ttgatactga
    16081 taaaactgtt ccgcgttagc cgacacgaag gaagcgttca gatccggttt tgttcccggc
    16141 gtggtgacgg gcacaaccgg tgaagtatga gtggatggcg ctttatcggt cgcggcattg
    16201 ttttccacct cttcattgac cagctttttg aactcttccg gtgccagatc gatttgctta
    16261 tacagcgagt cgaaacgttt actctcgatc actgaggaga acgtcaggca aacctgttct
    16321 gtattcgccg gacagctgtt actggacgtt accagcgaaa ccgcattgct tttcttgtca
    16381 tcggaagctg ccagttcttg cgcgtcctca agactaaacg acactttttc aaccgcacgc
    16441 aggctacgcg cagcttctga ctgggtgtcg aaggtagcaa ttgtcgtgtt acccgccacg
    16501 caagtaacgt cctcaccagg tgtaaatgta aagccatcgc tggattcacc attacaagta
    16561 gcaccagtta cccgctggct tccgcccagg gtcagataac ccgttttcgt aggaacaggt
    16621 tctggctccg gttctggctc aggatcaggt gtcggttccg gcgtaggttc tgggtccggc
    16681 gttggctcag gcgtcggctc cgggtttggt gttggatcag gtttcacttc cggcaaagac
    16741 cctgttccag aatctacagg cggcgtatcg gaggaagatc cggagccacc gccatcacaa
    16801 ccggctaaca gggttgcact caaaatagcc gctaaaagcg atttcttata tttaaatttc
    16861 ttattcatta ataacgcaag tgacaaaaca aagttaaaag attcgtaacc ggggaaatag
    16921 ttttatgctt tatcttcttt aataacttcc tccattctta agaaaacggc atcattgata
//
LOCUS       DAZFRE010000100        16722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFRE010000100 DAZFRE010000000
VERSION     DAZFRE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.100"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..789
                     /gene="yeeO"
                     /locus_tag="ACHKUI_003961"
     CDS             <1..789
                     /gene="yeeO"
                     /locus_tag="ACHKUI_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6600056.1"
                     /translation="RYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGI
                     PASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGR
                     RLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIW
                     LNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGV
                     WMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            890..965
                     /locus_tag="ACHKUI_003962"
     tRNA            890..965
                     /locus_tag="ACHKUI_003962"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:923..925,aa:Asn,seq:gtt)
     gene            1127..2398
                     /locus_tag="ACHKUI_003963"
     CDS             1127..2398
                     /locus_tag="ACHKUI_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6600057.1"
                     /translation="MSLNDAKIRSLKPTDKPFKVSDSHGLYLLVKPGGSRLWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGIRKMLALNINPAQQRAAERGSRMQEKMFKSVA
                     LEWHSSKKKWSQNTADRVLARLNRHVFPTIGHLPVTELKSRHFIELLKGIEEKGLLEV
                     ASRSRQHLSNIMRYAVHQGLIEINPAANLDGVTASPARRHYPTLPLERLPELLERIDS
                     YHQGRELTRLAVLLTLHVFIRSSELRYARWTEINFRNRIWTIPATREAIAGVRYSSRG
                     AKMRTPHIVPLSEQVISILKRIKEISGGYELVFPGYHDPYKPMSENTINKALRQMGYN
                     TKQDICGHGFRAMACSALMESGLWSQDAVERQMSHQERNTVRLAYIHKAEHMEARMDM
                     MQWWSDYLDMCSEIWVPPYIWSQQNINLAVT"
     gene            complement(2678..3190)
                     /gene="clbS"
                     /locus_tag="ACHKUI_003964"
     CDS             complement(2678..3190)
                     /gene="clbS"
                     /locus_tag="ACHKUI_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HFO6600058.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDELYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(3225..3947)
                     /gene="clbQ"
                     /locus_tag="ACHKUI_003965"
     CDS             complement(3225..3947)
                     /gene="clbQ"
                     /locus_tag="ACHKUI_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HFO6600059.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRSVLSDNITLRPLEPAGRGTRI
                     RQPLCLTMVDAVADLYQQFVKHYTGGDYAIFGHSLGGIMAFELVHYILDHGHDMPCAL
                     FFSGCRPPDRASHEVILHTLPDQAFMEEIVKLGGTPVDVFRNKELMTIFTPIIKNDYR
                     LYEQYVFQAKARTLTCPIVLFHGDADNLVMQDELLAWEKFTTRKTRTIIFPAADHFFV
                     DKHFEQVVGYVNQTIESLEIVG"
     gene            complement(3940..5454)
                     /gene="clbP"
                     /locus_tag="ACHKUI_003966"
     CDS             complement(3940..5454)
                     /gene="clbP"
                     /locus_tag="ACHKUI_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin peptidase ClbP"
                     /protein_id="HFO6600060.1"
                     /translation="MTIMEHVSIKTLYHLLCCMLLFISAMCALAQEHEPIGAQDERLS
                     TLIHQRMQEAKVPALSVSVTIKGVRQRFVYGVADVASQKANTLDTVYELGSMSKAFTG
                     LVVQILIQEGRLRQGDDIITYLPEMRLNYQGKPASLTVADFLYHTSGLPFSTLARLEN
                     PMPGSAVAQQLRNENLLFAPGAKFSYASANYDVLGAVIENVTGKTFTEVIAERLTQPL
                     GMSATVAVKGDEIIVNKASGYKLGFGKPVLFHAPLARNHVPAAYIHSTLPDMEIWIDA
                     WLHRKALPATLREAMSNSWRGNSDVPLAADNRILYASGWFIDQNQGPYISHGGQNPNF
                     SSCIALRPDQQIGIVALANMNSNLILQLCADIDNYLRIGKYADGAGDAITATDTLFVY
                     LTLLLCFWGAVVVVRGAFRVYRATAHGPGKQQRLRLRVRDYIIALAVPGLVAAMLYVA
                     PGILSPGLDWRFILVWGPSSVLAIPFGIILLAFVLTLNHQIKRILLHNKEWDDE"
     gene            complement(5467..7926)
                     /gene="clbO"
                     /locus_tag="ACHKUI_003967"
     CDS             complement(5467..7926)
                     /gene="clbO"
                     /locus_tag="ACHKUI_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbO"
                     /protein_id="HFO6600061.1"
                     /translation="MAKDDFTCGSLDIAIIGMSGRFSGAESVPEWWDKLLAGEEFTQP
                     TCTEDDNGNPWIRLRNIITAPYDFDAAFFNIPPGEALLMDPQQRIFLECCYNALEHAG
                     YIPTQLKRVGVYGATYANNYFIDRVYPYLKMSGDHHYLQAQIGNEKDYLCAQVAYKLG
                     FTGPAVSVQTACSSSLVAAYLACEGLLTFQADVALAGGVTLGFLQAHGYSPQGDKLVS
                     QDGHCAPFSAEATGTVYSSGAGVVVLKRLEDALRDQDRVYAVIKGGAVNNDGGRRLGF
                     VAPSVEGQVEAINTALAAAEVVPTDIALIETHGTGTPLGDEIELEALHRVFAPACAPH
                     SIQLGAVKANLGHLGVASGIVSLMKTALTLYTGLVPPQINLVNKHKKLLQPASPFYLS
                     DVVTSVPQTKRIHATVSSFGLGGTNAHLVLQNWCETPAQAVQENERRLFFFSAKTPLA
                     LRQQLDAHYHALATYAEADKDRIAYTLAQRRAHFPYRCALAADSVVALRASLAKLRDA
                     DMSFTPINMETTLVFLYPDRDDKLESALTHLLACQPNLRQRHQRLSQDVAQICEPADW
                     TPALRQFIQQVSLSEWLIEQSISPVQHIGYLTGAAAAQYVARIISLENAVQQVIVAET
                     TPEQTLAGNSELSEILANLAVTEGTLMLEIGRAGTFSILYHQHAQWVGQTVFSPMLNT
                     DTPEDILPLLGTLWQRGVTICLPEMPAVQTIGLPGYSFDRVRYEIQSSDARENAMLPV
                     SYLSVSDFVEKTWRSLLCIDHYDEHAVIFEYGATSMHVISFVDSCNHIYKIGLTAADI
                     YARPAIREHSEFISECVDGIL"
     gene            complement(7957..12324)
                     /gene="clbN"
                     /locus_tag="ACHKUI_003968"
     CDS             complement(7957..12324)
                     /gene="clbN"
                     /locus_tag="ACHKUI_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbN"
                     /protein_id="HFO6600062.1"
                     /translation="MMSGNPLSWPQEQCHIIDQLYPYSAVNIIGGVVTIEGIVDLPRL
                     HAAIQSAIRQFDALRMWFVMGEESEVVSQVQPYHWRDIRHLTFSPDYDKENLRPAAIE
                     TFVDEWFRQPFTLLAHDLFEFVTFTCGEQYSGYLFKAHHGIADGWSMALLSNHVKRAY
                     EQQDVPDDASPAYSAFLAQQQSYQASTRFAVDRGWWRDYIDEYRDCFPDSSPIVTTEG
                     ISCSTWLEPAMINRLYRLCNRYGCTLNTLFIALFALYRARVWGEEKGVIGVPLANRHT
                     REARRCFGMFTNQLPLAYRLVRTERFCERVAFFQRELKRGFKHSKYPITLFNQDLAEQ
                     GGGKLRAFDYCVNYYNFTYERHIAGAAQRVESYYSGEQSYKLQIVLQTVNNHKESLRL
                     SLEALRSAFTPHQLTAMKNGLLDLVTALDRQPDARLGDLEVYPAPHVALACGSLKPSF
                     TSRFAAQVVEHGDRTALIDNEQSLTYRQLDDAVERVARYLRQQGIGRGQVVGIIAEHS
                     AQTVMVIYGILRCGAAFLPLNPALPTTRLYAMCRKAQVAHILYDPAMHELTQALAFPA
                     SSLLQALATSALAREPWPAIEPQDLAYVLFTSGSTGEPKGVQVSHGNLANYLHFAAER
                     YFTAQDRAALYSSLSFDLTITTLFAPLCVGASISVCRHAESETLLRMAVVDQPNTVIK
                     LTPAHLRLLCAAGISSEQIRTLVVGGEDFKRDLARKAAALFPQAVIYNEYGPTEATVG
                     CMIYRYTGQETLPSLPIGMAIDGCQVAICSPWGCPVPEGETGELVIYGASVTQGYIDA
                     PQQTAAAYLKDTNGVMIGYRSGDIGYAIAPNTLVYQGRKDDQVKINGYRIELCEIEQA
                     LLSAPQVESAAVAVIDDVQGQHSGLLACVTPSSVDVATVMQHLRQQLPTYMQPKQCCA
                     IAQLPLSHNGKVDVRQMVATVRNTAPASGSERLGDAAIRHSVRVCVEGALEQTEFDDN
                     ENLYVLGLDSIKSIQIAAQLRHHGWTMSAVQVMECGTVNAICEFLASHTTVSQLAQYA
                     HNTRIDLPALRWFTQLALPVPNVYNHVIVLKVLPGCPLEQLHNRLHTLIQQQPALHSA
                     LDAEGRLLVCDPNVCYPNEVLTEYSTAQWTLAEVIAQCNSMLDVTNGRVFTAALLHAP
                     QPASSTLVLCAHHLCVDMHSWYLILSTLDAVSTVNGTSNSGLHRWNDYLASKTVDSAT
                     HESWRTVCQTLPLHFPPVSLPDDSLPRTRAWREDFRHPCVRRLFESSGNTAYSAETYV
                     LTALALVLRYYSEEPWCRIEMEGMGRGCWPDEPDVADTVGWFTLFYPWAIPLHGDMAT
                     LLSAIASDLAKRTHGGGDYGLLQMRHAPEDSLAQGIRMNYIGVQAQPSLRYFHIDHFN
                     SDIYTAPENALGCVLEFNIARSAADGLSFHCRFDPTRIALNDVQLLLARYKNSLTDLD
                     AWLCQHSATLTGAPTLWTL"
     gene            complement(12321..13760)
                     /gene="clbM"
                     /locus_tag="ACHKUI_003969"
     CDS             complement(12321..13760)
                     /gene="clbM"
                     /locus_tag="ACHKUI_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin export MATE transporter ClbM"
                     /protein_id="HFO6600063.1"
                     /translation="MTVNKLEPDNGTPNDELFTVAGMFDGSLYKLLLRMALPMFVGML
                     TQVTYAIADIFWLSHIDVTNSGIIAGVGLVFPVGMGLFAIANGIQIGMGSLLSRAIGM
                     QRLDRAQRILSVGIIIALFFAIVITVLGYVYAQPLLRSLGATKSIIGYATEFYYYSLL
                     TVFSIMLIGVMMGLFQGAGKIMVIMKASLLGALVNIMLDPIMIFVFDFGVKGVALASF
                     LAQLSMVAYFIYTLMGLHIGLSIRIALRPFSWKIYREFLSVGMAQMLMQLIIAVGIVI
                     YNFFIVRLDVNAMAAFTLTGRIDYFIITPMLAIATALLTVVGQNWGHGNVTRTLNAYW
                     AAVALAFSIVLVLAVMHIVLAPWMYPLFTRVVAVSDYAVLQTRIMALALPFVAISLLA
                     SEYYQAIGKPWYSVLLTLMRHVFISVPVVYLLAIVLEMRITGVYFGAMSGTFVAALLA
                     WRLLRLSPRLLRWNQEAVRSQHLDMEVAP"
     gene            complement(13822..15285)
                     /gene="clbL"
                     /locus_tag="ACHKUI_003970"
     CDS             complement(13822..15285)
                     /gene="clbL"
                     /locus_tag="ACHKUI_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis amidase ClbL"
                     /protein_id="HFO6600064.1"
                     /translation="MSEQSYRSAGTLLAQLASGETTSVALVNHYFSRMAQFNKPLNAV
                     VQQHYALALEAAARADRERLEGRARGVLHGLPCTVKESFDVQGWLTTSGAHYLKDNRA
                     TQDAPSIARLRAAGAILMGKTNVPMMTADWQTYNDLYGTTHNLWDRQRSPGGSSGGAA
                     VAVAADFTPVEFGSDLFGSLRIPAHYTGVYAHRCSLGLMSVRGHVPGGGPQATDEPDL
                     STAGPMARSAADLRLMMRALSTFWVEPPRIPDFSRYQAKANYRVCTWFSAPHHEIDQQ
                     IAQRFQSFIDKLRAQPGVEVDDAMPADIDPDALFDIAVKLSGRLVSTALNGRQRLTAG
                     LAALGFRLVGKLADVPEGITSYYQGMLKDSGEQRNTDKLRHEYSRVIETLFARYDVLL
                     TPVSPVLAFAHMQQPVRKRKLIVNGEPQDYNEHLFWNMLATVFGLPATVYPLAKTMDE
                     LPCGIQIISGHFHDDVTINFAEFCESISGGFTVPEGY"
     gene            complement(15278..>16722)
                     /locus_tag="ACHKUI_003971"
     CDS             complement(15278..>16722)
                     /locus_tag="ACHKUI_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFO6600065.1"
                     /translation="ERCIARHPDVEQSVVVAVGNSQHRRLVAFAKLHDRHQAQALQAK
                     EAEAAALAQGIIVNPAQRLAFKLKEPHIRALDGLGIALTAPADSTRYIKRRSYRHFSA
                     QKTTLAQLGQLLSGLGQMRLPGLPFAKYAYASAGGLYPVQTYVYLHPDKIEEGVSGIY
                     YFDPRQSCLMPVAPEVELNSGFHAGPNQSIADRAAFTLFMVADMAVISPFYGQEAAWH
                     FSVMEAGTLCHLLEEDAPRYGLGLCQLGMADFSAVASHFQLSPHHRYVHCTVGGAIGQ
                     EAASAAALLRDFSTYEKPKETAAPLDMQSYKDAMLRGLRQQLPDYMVPSDLMLATDFP
                     LTANGKLDRQKLQLQGEQIAHQRDGVGPIQVDSALQQRLVALWQEVLGVSHVSAEDDF
                     FSLGGSSIELVRIQQALEAIIGQEIPIVDLFRLPTIADVARYLDEQLHNLPAAHDIVL
                     AQAEVSQVSAARENLALRRKRAQQGEKGDE"
BASE COUNT         4170 a         4583 c         4115 g         3854 t
ORIGIN      
        1 cgttatattg gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta
       61 aggatttcgt taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg
      121 gggattggta ttccagcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc
      181 caaatgttcg ttgccgggat ggggaccagc gttattgccg gaaattttat cgcgttctca
      241 attgcggctc ttatcaactt acccggaagt gcgctcggtt ctgcttctac gatcattaca
      301 ggccgaaggt tgggggtagg gcagatagcg caagcagaga ttcagttgcg gcatgtgttc
      361 tggctgtcca ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt
      421 atggcatcgt tttacaccca ggatccacag gttaaacacg tcgttgtgat tctgatttgg
      481 ctaaatgctt tatttatgcc tatttggtcc gcctcatggg tgctacccgc tggatttaaa
      541 ggtgctcgtg atgcccgtta cgccatgtgg gtttcgatgt tgagcatgtg gggttgtcgg
      601 gttgtagtcg gttatgtgct gggaatcatg cttggctggg gtgtggttgg cgtctggatg
      661 ggaatgtttg ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga
      721 cgttggctat ggaaataccc tcgatccgag ccgcaaaagt gtgaaaaaaa gccagttgta
      781 tcggaataaa cgacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg
      841 ctttgacatt gtgggtgggc atcgctaata ttcgcctcgt tctcacgatt cctctgtagt
      901 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
      961 agccaatttc ctgctttcat gcatccttgc gaatccttat gttattttta ttcaacaggt
     1021 tagcgtgaaa actcttcccg gtgcattttg attttaccct ctgcatcggg aaaaattggt
     1081 ggtcaaatct ggggtcaggt tagttcgata atggagtgac ccccatatgt cccttaacga
     1141 cgcaaaaatc cgtagtctca agcccactga taaacccttt aaagtctccg attcccacgg
     1201 tctgtatctg ctggtcaaac caggcggttc ccgtctctgg tatcttaaat accgtattaa
     1261 tggcaaagaa tcccgtattg cactgggtgc ctaccctgcc gtctccctgt ctgacgcccg
     1321 ccagcagcgc gaaggcatcc gtaagatgct ggcgctgaac atcaacccgg cgcagcaacg
     1381 tgccgctgaa cgcggctcgc gcatgcagga gaaaatgttt aaatccgtgg cactggagtg
     1441 gcacagtagc aagaaaaaat ggtcgcagaa taccgcagat cgcgtgcttg cccgtctgaa
     1501 cagacatgtc tttccgacca tcgggcatct accagttacc gagcttaaat cacgtcattt
     1561 cattgaatta ctgaaaggca ttgaggaaaa gggcttgctt gaagttgcgt cccgctcgcg
     1621 gcaacacctc agcaacatta tgcgttatgc tgttcatcag gggttaatcg aaatcaaccc
     1681 agctgcaaat cttgacggcg tgacggcttc tcccgccaga cgacattatc ccaccctgcc
     1741 actggagcgc cttcctgagc tgcttgaacg gattgacagt tatcaccaag gacgggaact
     1801 caccaggctt gcagtcctgc tgacgttgca tgtgttcatt cgctcaagtg aactgcgcta
     1861 tgctcgttgg acagagatta acttcagaaa tcggatctgg acgatacctg ccacacgcga
     1921 agcgattgca ggcgtacgtt attccagtcg aggtgccaaa atgcgtacac cgcatattgt
     1981 gcccctttct gagcaggtta tctctattct gaaaaggatt aaggaaatat ctggcgggta
     2041 tgaactggtg ttccccggct atcatgaccc ctacaagccg atgagtgaaa atactatcaa
     2101 taaggcactt cgccagatgg gatacaacac gaaacaagat atctgcggtc atggtttccg
     2161 ggcaatggcg tgtagcgcgc tgatggaatc cggtttatgg tcgcaagatg cagtggagcg
     2221 tcagatgagt caccaagagc gcaacactgt gcgtctggct tacattcata aggccgaaca
     2281 tatggaagcc cgcatggaca tgatgcagtg gtggtcggat tatctggata tgtgcagtga
     2341 aatctgggtg cccccgtaca tctggagtca gcaaaacatt aatctcgcag taacctgact
     2401 tatgaataat cttgtaaaga accaggccgt atcagcaaac gctgatacgg cttttcatcc
     2461 acaggccgtt tttcatcttc taagtcgtcc aggacatcat ggttcttaat acacatgcgt
     2521 tcgcatgtgc ttatgttaag tagacttatt gtcttacaca ttttatccgt atcattaacc
     2581 agaatatgtt ccggagcagc gggtgatagt gccgccagtc cacactcatc gctgcccgga
     2641 aaaatcatca gtggggaggc aaacggtaag caccccgcta ttctgcaaga catttctgca
     2701 gtttatttaa cctccccgaa gcatttttat aaggcgaggc ggtattaaat tgaatcatac
     2761 gacctcgggt ccatttatta taccaaggtt taccgtaaag ctcgtcgtta ctgaatcgtt
     2821 caatcaaagc cacgagttgc tgcttatttt cctttaaccg tgccaataaa acttcgtact
     2881 ctgtaatatc ctcatagtca cggtagaatt tctgtgctaa acgccccaat tcattccatt
     2941 taaatccttc ctcaggaaaa ataatagttt ttccttttgc ctcttggtca tgccagtgta
     3001 acaccagctc tccccagcca atcagatagg aaaccagatt cgctacgcta atcgtagtcc
     3061 ccttcgcgtg cccttccaat agaggttcaa aggcgagttg gggcgtaata gattctagct
     3121 tcttatttaa taaagaaaaa ttactattaa tagctttaat taactcttct tttgatgatg
     3181 gaacagccat atctattgct ccttgtatag ttacacaact atttctaccc tactatttcg
     3241 agtgattcaa tcgtctggtt cacataacct accacctgtt caaaatgctt atcgacaaaa
     3301 aaatgatcgg cagcaggaaa tataatagtc cgcgtctttc gtgtggtgaa tttttcccat
     3361 gcaagtaatt catcctgcat taccagattg tcagcatcgc catgaaatag cacgatcgga
     3421 caggttaatg tgcgcgcctt ggcctgaaat acatactgct catagagccg ataatcgttt
     3481 ttaatgatgg gggtgaaaat tgtcattaac tctttattac gaaagacatc aaccggagtt
     3541 ccgcccagct tgacgatctc ttccataaac gcctgatcgg gcaaggtatg cagtattact
     3601 tcatgagagg cccgatcggg tgggcgacag ccggaaaaaa acagcgcgca tggcatgtca
     3661 tgtccatgat cgagaatata atgcaccagt tcgaaggcca tgatccctcc gagactatgc
     3721 ccaaaaatgg cgtagtctcc acctgtgtag tgtttcacaa attgttgata aaggtcagcg
     3781 acggcatcca ccatcgtaag acacagcggc tggcgtattc tagttcccct ccccgcaggt
     3841 tctaaaggcc gcaaagtaat attgtccgac agcacgctac gccatttata atacatggcg
     3901 gcagaaccac ctgaatatgg caaacaatac aaactgatat tactcatcgt cccactcctt
     3961 gttgtgtaat agaattcgtt taatttgatg atttaatgtc agaacgaaag ctaacaggat
     4021 aattccgaac ggtatcgcca acacgctcga tggaccccat accaagataa aacgccagtc
     4081 aagtcctgga gatagtatac ccggtgcgac atagagcatg gcggccacga gcccaggaac
     4141 cgccaaggcg atgatatagt cacgtacgcg taaacgtaac ctctgctgtt ttccagggcc
     4201 atgcgccgtt gcgcgataaa cacggaaagc accgcgcact acaaccaccg ccccccaaaa
     4261 acacagcaac aacgtgaggt agacgaaaag ggtatcggtg gctgtaattg catcaccagc
     4321 gccgtcagca tatttgccaa tgcgcagata attatcgata tccgcgcaaa gctgtagtat
     4381 cagattcgaa ttcatatttg ccagcgcaac aatgccaatc tgctgatccg gtcgcaacgc
     4441 aatgcaagaa gaaaagtttg gattctgccc accgtgactg atgtaagggc cttgattctg
     4501 gtcgataaac caaccgctgg catagaggat acgattgtct gcggcaagcg gaacatcact
     4561 attaccacgc caactgttac tcatcgcctc acgcagcgtt gccggcaaag cctttctgtg
     4621 caaccaggcg tctatccata tttccatatc aggcagagtg ctatggatat aggcggcagg
     4681 aacatggttc cgggccagag gcgcatgaaa cagaacgggt ttgccgaatc ccagtttata
     4741 gccgcttgcc ttgttgacaa taatctcatc ccccttaact gccacagtcg ccgacatgcc
     4801 cagcggctgc gtgagtcgtt ccgcaatgac ctctgtaaag gtttttcccg tcacattttc
     4861 aatcaccgcg cccaacacat cataattggc ggaggcatag ctaaacttcg cacccggcgc
     4921 aaacagcaga ttctcgttgc gcagttgctg tgccacagcg ctcccaggca tagggttttc
     4981 cagccgagcc agtgttgaaa aaggcaatcc tgatgtatga taaaggaaat cagccacggt
     5041 cagggaagca ggttttccct gataattcaa gcgcatttcc ggcagatagg taatgatatc
     5101 atccccttgc cggagtctgc cttcctgaat cagtatttgc accacaagtc cggtaaacgc
     5161 cttactcatc gatcccagct cgtaaactgt gtctagagta ttcgctttct gactagccac
     5221 atcggcaaca ccgtagacaa atcgctgacg taccccctta atggtcacac ttacggaaag
     5281 ggctgggacc ttggcctcct gcatccgttg gtgaattaat gtcgacaggc gctcatcttg
     5341 cgccccgata ggctcatgtt cttgcgccaa agcgcacata gcggaaataa agagcagcat
     5401 acagcacagg agatgatata atgttttaat gctaacgtgt tccattattg tcatcctgtg
     5461 aacacctcat aaaataccat caacacattc ggagataaat tcactgtgct cacggatagc
     5521 gggtctggca taaatatcgg cggcagtcag tccgatttta taaatgtggt tacagctgtc
     5581 gacgaaagag ataacgtgca tcgaggtcgc tccgtattcg aaaataaccg cgtgttcgtc
     5641 ataatgatcg atgcataaca atgaacgcca ggttttctcc acaaagtcgc tgaccgacaa
     5701 ataactcact ggtaacatag cgttctctct cgcatcactg gactgaattt cataccggac
     5761 gcgatcaaac gaataaccgg gtaagcctat agtctgcacg gctggcattt ccggtaaaca
     5821 aatagtcact cctcgttgcc aaagagtgcc caacagcggc aggatgtcct caggcgtatc
     5881 cgtattcagc atgggggaaa atactgtttg cccaacccac tgcgcatgct ggtgatacaa
     5941 tatggaaaac gtccccgcgc gaccgatttc cagcatcagc gttccctccg tcaccgccag
     6001 attagccaat atttcgctca gttcactgtt gccggccagc gtctgctccg gtgtcgtttc
     6061 agccacaatc acctgctgaa cagcgttttc cagtgagatg atgcgcgcaa catactgcgc
     6121 tgctgcagcc cccgtcaggt aaccgatgtg ctgcacaggc gagatacttt gttcgatcag
     6181 ccattcacta agactcacct gctggataaa ctgacgcaat gctggcgtcc aatcggctgg
     6241 ttcgcagatt tgtgcaacat cctgagacag gcgctggtgc cgctggcgca agttaggttg
     6301 acaagccagc aaatgcgtca gggcactctc cagcttatcg tcccggtccg gatataggaa
     6361 caccaaggta gtttccatat tgataggggt aaaagacatg tcagcatccc gcagtttcgc
     6421 caaacttgca cgcaacgcta ccacactgtc tgccgccagc gcacagcgat acgggaaatg
     6481 tgcccggcgt tgcgcgagag tataggcgat acgatcttta tctgcttccg cataggtcgc
     6541 cagagcgtga taatgagcat ccaactgttg tctaagggct aatggcgttt tggcgctgaa
     6601 gaaaaacagg cggcgctcat tttcttgcac cgcttgcgca ggcgtctcgc accagttttg
     6661 cagaaccaaa tgagcattgg tccctcccag cccaaacgaa ctaacggtgg catggatcct
     6721 tttcgtttgt gggacagaag tcactacatc actgagataa aatggcgagg ctggctgcaa
     6781 cagtttttta tgtttattta ctaggttgat ctgcggggga actaaaccag tatatagcgt
     6841 cagtgcggtt ttcattaagc tgacgatacc ggaagcgact cccaggtgac ccaagttggc
     6901 ttttaccgca cccaattgaa tgctgtgcgg cgcacatgcc ggtgcgaaaa cccggtgcag
     6961 agcttcaagc tcaatctcat cacccaacgg ggtaccggta ccgtgcgttt cgatcagtgc
     7021 gatatcagtc ggtacgactt ctgcggcggc aagcgccgta ttgatcgctt ctacctgtcc
     7081 ttctacgctg ggggcgacaa accccagccg acgcccgccg tcgttattta ccgcacctcc
     7141 tttaatcacc gcatacactc tgtcttggtc gcgtagtgca tcctcaagac gttttagcac
     7201 cacaactccc gcaccgctgc tgtataccgt gccggtcgcc tcagcgctaa acggggcgca
     7261 gtgtccgtct tgcgatacca gcttatcacc ttgcggactg tagccgtgcg cctgtaaaaa
     7321 acccaaggtg accccacctg ccaacgcaac atcggcttga aaagtcaata acccctcgca
     7381 tgctaaatat gctgccacca acgaactgga acaggcggtc tgtacactca cagcgggtcc
     7441 ggtaaacccc aacttgtagg caacctgagc acacaagtaa tccttttcgt taccaatctg
     7501 cgcctgaaga taatggtgat cgccgctcat cttcaggtat ggatagacgc gatcgataaa
     7561 atagttattg gcgtaggtcg ccccatagac gccgactcgc ttaagttgcg tgggaatata
     7621 gcccgcatgc tcaagagcgt tgtagcagca ttcaagaaat attcgttgtt gcggatccat
     7681 caacagggct tcgccaggcg gaatattgaa aaatgcagca tcgaagtcat agggagcggt
     7741 gattatattg cgcagcctaa tccaagggtt cccattatcg tcttctgtgc atgtcggttg
     7801 agtgaactcc tcacccgcca gcaacttatc ccaccactcg ggcaccgatt ccgcaccgga
     7861 aaaacgcccg ctcatgccaa taatggcaat atccagtgag ccacaggtaa aatcatcctt
     7921 tgccattgtt gtattcctta agggttggca tcgtgtttat agtgtccaca aagtcggcgc
     7981 gcccgtcagc gtggcgctgt gttggcacag ccaggcatcc agatcagtga ggctgttctt
     8041 gtagcgcgcc agcaggagtt gcacatcatt cagcgcaatg cgcgtgggat cgaaacggca
     8101 gtggaaggag agaccatcag ctgccgagcg cgcaatatta aactccagca cgcagccgag
     8161 cgcgttttca ggcgcggtat agatatcact attaaagtga tcaatatgaa aatagcggag
     8221 tgaaggttgc gcctgcacac cgatgtaatt cattcggatc ccttgcgcaa gagagtcttc
     8281 cggtgcatgc cgcatttgca gcagaccgta atctccccca ccatgcgtgc gttttgccaa
     8341 atcggaagcg atcgcagata acagtgtcgc catatcgcca tgcagaggga tcgcccaagg
     8401 ataaaataga gtaaaccacc cgaccgtatc cgcaacgtca ggctcatcgg gccagcagcc
     8461 gcgtcccatg ccttccatct cgatgcgaca ccaaggctct tcgctgtagt aacgcagcac
     8521 cagcgccaat gccgttagca cataggtttc ggcgctgtac gcggtattac cactggattc
     8581 aaacaacctc ctgacgcaag gatggcgaaa gtcttcacgc caagcgcgcg tacgaggtaa
     8641 tgagtcatct ggcaatgaca cgggtgggaa atgcagcggt agcgtttgac atactgtgcg
     8701 ccagctttcg tgtgtggcgg aatctaccgt tttgctcgcc agatagtcat tccagcgatg
     8761 cagtccgctg ttgctggtgc cattgacagt gctgacggcg tccagcgtgc tgaggattaa
     8821 ataccaagag tgcatatcga cgcacaagtg atgggcacag agtaccagcg tgctagacgc
     8881 aggctgcggg gcgtgtaaca gagctgccgt aaagactcga ccatttgtca cgtcaagcat
     8941 gctattgcac tgagcgatca cctctgccaa cgtccactgc gccgtcgaat attccgtgag
     9001 cacctcgttg ggataacaca cattagggtc gcatactagc aaccgcccct cagcgtccag
     9061 cgcactgtgc aaagctggct gctgctggat taatgtatgc agcctgttgt gtaactgctc
     9121 gagcgggcag cccggcaaca ctttcagcac aatgacatgg ttataaacat tgggcactgg
     9181 caacgccaac tgcgtaaacc aacgcaatgc gggtagatcg atacgggtgt tatgagcata
     9241 ctgcgcgagc tgtgacaccg ttgtatgact agcgagaaac tcacagatgg cgttcactgt
     9301 tccacactcc atcacctgca ccgctgacat cgtccagccg tggtgcctga gctgcgctgc
     9361 aatctggata ctcttgatgg agtccaaccc caagacataa agattttcgt tatcatcaaa
     9421 ctcagtttgc tccagcgccc cttccacaca tacccgaact gaatgcctta tcgccgcatc
     9481 tcccagccgc tcgctacccg atgcaggcgc ggtgtttcgg accgttgcga ccatctgacg
     9541 cacgtccacc ttgccattgt gcgacagcgg cagttgggcg atagcacagc actgcttggg
     9601 ttgcatgtag gtgggtaatt gctgacgcag atgttgcatt acggtagcta catcaacaga
     9661 tgacggtgtc acgcaggcta gcaacccgct gtgctgcccc tgcacatcat caatcaccgc
     9721 caccgccgca ctttctacct gaggtgcgct caacaacgct tgttcgattt cgcacagctc
     9781 aatacgatag ccattaattt tgacctgatc gtctttacgt ccctgataaa ccagcgtatt
     9841 aggagcgatg gcgtagccaa tatcaccgct gcgatagcca atcatcaccc cattggtatc
     9901 tttaaggtag gcggcggccg tttgttgcgg cgcatcgata tagccctgcg tcactgacgc
     9961 gccataaatc accaattcac cagtttcgcc ttccggtacc ggacagcccc aaggggagca
    10021 aattgctacc tggcaaccgt cgatcgccat accgatgggc aatgaaggca gcgtttcttg
    10081 cccggtgtag cgatagatca tgcaaccaac ggtggcctct gtcggcccat attcgttata
    10141 gatcacggcc tgaggaaaga gagcagccgc cttacgcgcc agatcccgct tgaaatcctc
    10201 cccgccaacc accaaggtac gaatctgctc actgcttatg ccagccgcac acaacaaccg
    10261 caggtgtgca ggcgtcagtt tgatcactgt gttgggctga tcaaccacgg ccatgcgcag
    10321 caatgtttcg ctctcggcgt gccgacaaac gctgatgcta gcgcccacac ataagggggc
    10381 gaacagcgta gtgatggtca aatcgaagga cagcgaagag tagagcgcag cccggtcctg
    10441 cgctgtaaag taacgttcgg cggcgaaatg gagatagtta gccagattgc catgagagac
    10501 ttgcacgccc ttaggctcgc cggtcgatcc ggaggtaaac agcacatatg ccagatcctg
    10561 cggttcaata gctggccaag gttctctagc aagcgccgaa gtggctaagg cctggagcaa
    10621 gctggacgcg ggaaacgcca gcgcctgcgt caattcatgc atcgccggat cgtaaaggat
    10681 atgcgctacc tgcgctttac gacacatggc gtaaaggcgg gtcgttggca acgcgggatt
    10741 tagcggcaga aatgccgcgc cgcagcgcaa aataccataa ataaccatca ccgtttgagc
    10801 gctatgctca gcgataatcc caaccacttg accgcgtccg atcccctgtt ggcgcagata
    10861 acgcgcaacc cgctcaacag cgtcatccaa ctgccgataa gtcaaagact gttcattatc
    10921 aattagtgct gtgcgatcac catgctccac aacttgcgct gcaaatcggg aagtaaatga
    10981 gggtttgagt gagccacatg ccaatgcgac gtgcggcgca ggatagactt cgagatcgcc
    11041 tagacgagcg tcaggttgcc gatccaatgc agtgactaaa tccaacagcc catttttcat
    11101 cgctgtcagt tggtgcggag tgaaagcgct acgcagcgcc tccagactca acctaaggct
    11161 ttctttatga ttattaacag tttgcaaaac aatctgtagc ttataggact gctcgccgct
    11221 gtagtaactc tccacgcgtt gcgcggcacc tgcaatgtgg cgttcatagg taaagttata
    11281 ataattaacg caataatcga acgcccgcaa cttccctccc ccctgttccg ccaaatcctg
    11341 gttgaataag gtaatgggat atttgctgtg tttaaaaccg cgtttcagtt cgcgctggaa
    11401 aaaggccacc cgttcacaga atcgttccgt gcgcaccagt cgataggcca gcggcaattg
    11461 attagtaaac atgccaaagc aacgccgcgc ctcgcgagta tgccgattgg ctaacggcac
    11521 gcctattacg cctttttcct ccccccatac tctggcgcga tagagagcga agagtgcaat
    11581 aaacagcgta ttaagcgtac agccataacg attacacaac ctgtagagac ggttaatcat
    11641 ggctggctca agccaagtgg aacaactgat gccctcagta gtaacgatgg gggaactgtc
    11701 tggaaagcag tcgcggtatt cgtcgatata gtcacgccac cagccacgat caacggcaaa
    11761 gcgcgttgac gcctgataag attgctgctg cgccagaaat gcgctatagg ctggtgacgc
    11821 atcatctggt acgtcctgct gttcgtatgc tcgcttgaca tggtttgaca acaacgccat
    11881 tgaccaacca tcagcaatgc cgtgatgggc tttaaataaa taaccgctgt attgttcacc
    11941 gcacgtaaag gtgacaaact cgaacaaatc gtgcgccagc agggtaaagg gctgccggaa
    12001 ccactcatca acaaatgttt cgatcgccgc tgggcgtagg ttctccttgt cataatctgg
    12061 ggaaaaagtc aaatggcgga tatcgcgcca gtgataaggc tgcacttggc tgaccacttc
    12121 actctcctct cccatcacaa accacatacg caacgcatca aattgccgaa tggcggactg
    12181 gatggccgcg tgtagtcggg gcagatcgac aattccctca atggtgacca caccaccaat
    12241 gatgtttacg gcactgtaag gatacagttg atcaatgatg tggcactgtt cctgtggcca
    12301 cgacaacgga ttgcccgaca tcatggtgcc acctccatat ccaagtgttg ggagcgcacc
    12361 gcctcctgat tccatcgcag cagacgaggt gaaagacgca gcagacgcca cgccaacaac
    12421 gcagccacaa aagtgccgct catcgcccca aaatagacgc cggtaatacg catctctaac
    12481 acaatcgcca gcaggtatac gacagggaca gatataaaca cgtgacgcat cagcgtcaac
    12541 aacaccgaat accaaggctt accaatggcc tgataatatt cacttgctaa caggctgata
    12601 gcgacaaagg gtagtgccag tgccatgata cgggtttgca gtaccgcata gtcactcacg
    12661 gcaacaacac gagtaaacag gggatacatc cagggtgcca gtacaatatg cataacggct
    12721 aacaccagca caatagagaa ggccagcgcg acggcggccc aataggcatt cagcgtgcgg
    12781 gtgacatttc cgtgtcccca gttttgcccc accacagtca gcaacgcggt cgcgatagcc
    12841 agcatcggcg tgatgatgaa atagtcgatg cgaccagtga gcgtaaacgc tgccatcgcg
    12901 tttacgtcca gcctgacgat aaaaaaatta taaatcacta tgccgaccgc aatgatcagt
    12961 tgcatcagca tctgcgccat gccgacggag agaaactctc ggtagatttt ccaggagaat
    13021 gggcgtaacg cgatgcgtat actcaacccg atatgtaatc ccatcaatgt gtagatgaaa
    13081 taggcgacca tactcagttg cgccagaaac gacgccaatg ccaccccttt cacaccaaaa
    13141 tcgaatacaa agatcatgat tggatcgagc ataatattta ccaacgcccc caacaatgag
    13201 gccttcatga tgaccatgat cttacccgcg ccttgaaaca gccccatcat gacgccaatc
    13261 aacatgatgc tgaacacggt cagcagcgaa taataataga actccgttgc gtagccgatg
    13321 atgctcttcg tggcccccag tgagcgcaat agcggctggg cataaacata gccaagtacg
    13381 gtgatcacga tcgcaaagaa tagcgcgatg atgataccga ccgacaaaat ccgctgtgca
    13441 cgatccaacc gctgcatgcc gatggcgcgg gacagcaaag atcccatacc gatttgaatg
    13501 ccgttggcga tggcaaataa ccccattcca acggggaaaa ccagccccac accggcaatg
    13561 atgccgctgt tggtgacatc aatgtggctc agccaaaaaa tatcggcgat ggcataggtc
    13621 acctgcgtta acataccgac aaacatcggc aacgccatgc gcaggagtaa tttatacagc
    13681 gagccgtcaa acatacccgc caccgtgaac agctcatcgt tcggcgtgcc gttgtctggc
    13741 tcaagcttat tcaccgtcat acctgctcct tattcattaa atgcgccgca cctttattgg
    13801 cgctgtcacc gatatccgcc tctagtaccc ttccggtacc gtgaatccgc cactgatgct
    13861 ttcgcagaac tcagcaaagt taatcgtcac atcatcgtga aaatgcccgg aaatgatctg
    13921 tatgccgcac ggaagctcat ccatcgtttt cgccaatggg taaacggtgg caggcaaacc
    13981 aaaaaccgtt gctaacatgt tccagaacaa atgttcgttg taatcttgcg gttcgccatt
    14041 gacgataagc ttgcgcttac gcaccggctg ctgcatgtgc gcaaacgcca gcaccgggct
    14101 gacaggtgtc aacaacacgt cataacgcgc gaaaagggtt tctatcaccc ggctgtattc
    14161 gtggcggagt ttgtctgtat tccgctgttc gccactgtct ttcagcatgc cctggtaata
    14221 gctcgttatc ccctcaggca catccgccag cttgcccaca agcctgaagc ccagcgcagc
    14281 cagcccggct gtaagacgct ggcgtccatt gagcgccgta cttaccagcc gaccactgag
    14341 tttgactgct atatcaaaca gtgcgtcggg atcgatatcg gcgggcatag catcatcaac
    14401 ctccacgcca ggctgtgcgc gtaacttgtc gataaacgat tgaaaacgct gtgcgatctg
    14461 ttgatctatc tcatggtggg gcgcactaaa ccacgtgcac acgcggtagt ttgctttggc
    14521 ctgataacgg ctaaaatcgg gaatacgcgg cggttccacc cagaatgtgc tcaatgcgcg
    14581 catcatcaga cgcagatccg ccgcgctgcg cgccatgggg cccgccgtcg agagatccgg
    14641 ctcatcagtc gcttgcggac caccaccggg cacatgtcct ctgaccgaca tcaaccccaa
    14701 gctacaacga tgggcataga cacctgtgta gtgtgcggga atgcgcagtg agccaaacaa
    14761 atcgctgcca aattccacgg gggtgaaatc cgccgccacc gccaccgccg ctcctccgga
    14821 cgatccgccg ggtgaacgtt gcctatccca caggttatgg gtggtgccat acagatcgtt
    14881 ataggtttgc caatcggcag tcatcatcgg cacattggtt tttcccatca ggatcgcccc
    14941 ggcagcgcgt aagcgtgcga tggaaggagc atcctgcgtc gcccggttat ctttcagata
    15001 atgggcaccg gacgtggtta accacccttg cacgtcaaac gactccttaa cagtacaagg
    15061 taatccgtgc aacacgcccc ttgcgcgccc ctccagtcgc tcacgatcgg cgcgcgcggc
    15121 ggcctccaac gccaacgcat agtgctgctg caccaccgca ttcaacggct tgttaaactg
    15181 cgccatacgt gaaaaatagt gattcaccaa cgccactgag gtggtttctc cggacgccaa
    15241 ctgtgccaac agtgtccctg cgctacgata gctctgctca ctcatcgccc ttttctccct
    15301 gttgggcacg tttacgccgc aacgccagat tctcgcgcgc agcgctgacc tgcgaaacct
    15361 cagcctgcgc taatacgata tcgtgggcgg cgggtagatt gtgcagttgc tcatcaaggt
    15421 aacgcgccac atccgcgatg gttggcagac ggaacagatc gacaatggga atctcctgcc
    15481 cgataatcgc ctcaagtgcc tgctgaatac gcaccaattc tatagaactg ccccccagcg
    15541 agaagaaatc gtcttcggcc gacacgtggc tcacgccgag tacctcctgc cagagcgcca
    15601 ccagccgctg ttgtaacgca ctgtccacct ggattggacc cacgccgtca cgctggtggg
    15661 caatttgttc gccctgcagc tgtaattttt gccgatctag cttgccgtta gcggttaacg
    15721 gaaaatcggt cgctaacatc agatcactcg gcaccatata gtcaggcagt tgctgacgca
    15781 ggccgcgcag catggcatct ttgtagctct gcatgtccag cggcgcagcg gtttccttcg
    15841 gtttctcata ggtggagaaa tcgcgcagca atgctgcagc acttgccgcc tcttgcccta
    15901 tcgcgccccc cacggtgcaa tggacatagc gatgatgtgg cgacaattga aaatgcgatg
    15961 ccacagcgga aaagtctgcc atcccaagtt ggcacagccc caatccgtag cgcggcgcat
    16021 cttcttccag taaatggcag agagtacctg cttccatcac cgagaagtgc caagctgcct
    16081 cttgcccata gaatggcgag atcaccgcca tatcagccac cataaacagc gtgaatgccg
    16141 cccgatcggc aatagactga ttaggtccgg catgaaaacc actgttcagc tcgacttctg
    16201 gtgctaccgg cataagacag ctctgtcgcg ggtcgaagta gtaaataccg gatactccct
    16261 cttcgatctt gtctggatgc aggtacacgt aggtttgcac cgggtatagc cccccggcgg
    16321 acgcataggc atacttggca aaaggtagac cgggtagacg catctgcccc aagcccgaca
    16381 gcaattgccc caactgtgcc agcgtggttt tttgcgcgct gaaatgacga tagctgcggc
    16441 gtttgatgta acgtgtgcta tccgccggtg ccgtcagtgc aatgcccaga ccatccagcg
    16501 cgcgaatatg tggctccttg agtttgaacg ctagacgctg tgccggattc acaataatac
    16561 cctgcgccag tgccgccgcc tccgcttcct tagcttgcaa tgcctgcgcc tggtggcgat
    16621 cgtgcagttt ggcaaaagcg accagccgac gatgttgaga attacccact gccaccacca
    16681 ctgactgctc cacatcggga tgtcgcgcaa tgcagcgctc ga
//
LOCUS       DAZFRE010000101        16212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFRE010000101 DAZFRE010000000
VERSION     DAZFRE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.101"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            67..324
                     /gene="bhsA"
                     /locus_tag="ACHKUI_003972"
                     /gene_synonym="ycfR"
     CDS             67..324
                     /gene="bhsA"
                     /locus_tag="ACHKUI_003972"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6600066.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(406..1368)
                     /gene="ldtC"
                     /locus_tag="ACHKUI_003973"
     CDS             complement(406..1368)
                     /gene="ldtC"
                     /locus_tag="ACHKUI_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6600067.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1511..4957)
                     /gene="mfd"
                     /locus_tag="ACHKUI_003974"
     CDS             complement(1511..4957)
                     /gene="mfd"
                     /locus_tag="ACHKUI_003974"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6600068.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHNFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLINTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5085..6158)
                     /gene="ycfT"
                     /locus_tag="ACHKUI_003975"
     CDS             complement(5085..6158)
                     /gene="ycfT"
                     /locus_tag="ACHKUI_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO6600069.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAISALNQWLAPERNLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6419..7618
                     /gene="lolC"
                     /locus_tag="ACHKUI_003976"
     CDS             6419..7618
                     /gene="lolC"
                     /locus_tag="ACHKUI_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6600070.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7611..8312
                     /gene="lolD"
                     /locus_tag="ACHKUI_003977"
     CDS             7611..8312
                     /gene="lolD"
                     /locus_tag="ACHKUI_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6600071.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVLFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8312..9556
                     /gene="lolE"
                     /locus_tag="ACHKUI_003978"
     CDS             8312..9556
                     /gene="lolE"
                     /locus_tag="ACHKUI_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6600072.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9585..10496
                     /gene="nagK"
                     /locus_tag="ACHKUI_003979"
     CDS             9585..10496
                     /gene="nagK"
                     /locus_tag="ACHKUI_003979"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6600073.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10512..11333
                     /gene="cobB"
                     /locus_tag="ACHKUI_003980"
     CDS             10512..11333
                     /gene="cobB"
                     /locus_tag="ACHKUI_003980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6600074.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVCCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11471..12259)
                     /gene="ycfZ"
                     /locus_tag="ACHKUI_003981"
     CDS             complement(11471..12259)
                     /gene="ycfZ"
                     /locus_tag="ACHKUI_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO6600075.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12256..12717)
                     /gene="ymfA"
                     /locus_tag="ACHKUI_003982"
     CDS             complement(12256..12717)
                     /gene="ymfA"
                     /locus_tag="ACHKUI_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO6600076.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12775..13821)
                     /gene="potD"
                     /locus_tag="ACHKUI_003983"
     CDS             complement(12775..13821)
                     /gene="potD"
                     /locus_tag="ACHKUI_003983"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6600077.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13818..14612)
                     /gene="potC"
                     /locus_tag="ACHKUI_003984"
     CDS             complement(13818..14612)
                     /gene="potC"
                     /locus_tag="ACHKUI_003984"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6600078.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14779..15897)
                     /locus_tag="ACHKUI_003985"
     CDS             complement(14779..15897)
                     /locus_tag="ACHKUI_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6600079.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(15866..16135)
                     /locus_tag="ACHKUI_003986"
     CDS             complement(15866..16135)
                     /locus_tag="ACHKUI_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6600080.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
BASE COUNT         3869 a         4059 c         4186 g         4098 t
ORIGIN      
        1 tatcatttag ttatcgatcg ttaagtaatt gcttgcgacg tcattcatct gcataaggcc
       61 actattatga aaaacgtaaa aaccctcatc gctgcggcga ttttaagctc catgtcattt
      121 gccagctttg cggctgtcga agttcagtcc acgccagaag gccaacaaaa agtcggtact
      181 atcagtgcta acgcggggac aaatctggga tcgctggaag agcagctggc gcaaaaagcg
      241 gatgagatgg gcgcaaaatc tttccgtatt acttctgtaa ccggtccgaa taccctccat
      301 ggaacagcag taatttataa ataagcatta accctcatta atgcctgcta ctgctgattt
      361 tttccccgcg acatgctgtg tcgcggggat ttttttatcc gagctttaca gagtttgtgg
      421 gctcacttga tgacggcgga catccaccgg catcccggaa cggacatcca tcacatgttg
      481 catcacttca gcgtcagttt gtgctgcatc tttaaatgat tgcattgcgc tattcagcgt
      541 aattggcagc agctgcggat cgtcatcaat cttctctgac agcggctgat gtacttcaac
      601 cagacgcgca ccgtttggtt cggcagagac tttaatcggg gtgttgatga tattcacttt
      661 ggtgcctggg gtgacctggc taaagagtgt tttgatatcg tcatcccgca gacgaataca
      721 gccagaactt acccgcatgc caatgccgaa atcagcgttc gtaccgtgaa gcaaataaac
      781 gccgccatag gccgccagac gaatcgcatg atggcccatt gggttatccg gtccagccgg
      841 aactaccgca ggcaactcaa ttccctgtgc cttataccgc gcgcgaatgt ttgccgttgg
      901 cgtccaggtt gggtttgcac gcttgtcaga aacggtggtc accattgtcg gtgtcagcgt
      961 gtcaccacct aactgaccaa tgccgatggg atatacggtt accgaatttt ttcccggcgg
     1021 ataataatag agacgcagct ccgcaatgtt gatcacaatg ccttcgcgcg gcgcgtctgg
     1081 aagtagggtt tgcaacggga tcgttaacac gctacccgcg cgcggtacgt aaggatcaac
     1141 gccggggtta gcctgtaaca gagcgagaaa gccgacgtta tattttttgg cgatcgcttc
     1201 cagagaacca ccgtcatttt ccaccacatg aaatttgttt tcaccaacca gacgactgcc
     1261 cgctgacggc agcggccagg tgttggcttt tgccggtagc gccagcgcca cggcagcggc
     1321 gaacgtaaaa aacgttagcc agcgagaaaa acgcgttttg atcatcacca aagtccataa
     1381 taaatataag gttattgtaa taaaaaggta agtctgaatt atggagggtg acagtgtcgg
     1441 ataatgcagg gaagtgcaaa gaatttgtaa atattgcaga tgggggcgca gaaacgcccc
     1501 cgatttacca ttaagcgatc gcgttctctt ccagttcacg cataaactgg cgtacccatt
     1561 cgatacgcgt tttccgctcg ctcaaatcct gaataaactt cagacgcgtc ggaccatcaa
     1621 ggcggtaatg ctgcggctgt ttttgcagca aaccaatcaa ccaggccgga ttaacgtgat
     1681 tcttctcggc aaattcgatc acgccgcctt tctcattacc ttccagcttc ctgatcccca
     1741 gtttctgcgc ttgctggcgc agacgggcaa tatccagcag ggtacgcgcc ggatccggca
     1801 gcaggccgaa gcgatcgata agctcgactt tgatctcttc cagttcgttt tccgttttgg
     1861 cgctggcaat acgtttgtag aacgacagac gcgtgttcac gtcagggatg aaatcatctg
     1921 gcaatagcga cggcatccgc agctcgactt ctgtttgctg gctggtgaga tcttccagcg
     1981 acggctcgcg tccggctttc agcgcatcga cggcgttttc cagcaactcc atatacagtg
     2041 agaaaccgat ggtttccatt gagccgcttt gttcttcgcc aagcagttca cctgcgccgc
     2101 ggatctccag atcgtgcgtt gccagcgcaa aacctgcacc gagatcttcc agcgaggcaa
     2161 tcgcttcaag acgtttttgc gcatcggtag tcatcgcttt tggatgcggc gtcagtagcc
     2221 atgcatatgc ctggtggtga gaacgcccga cgcgaccgcg caactggtgc aactgtgcca
     2281 gaccgaagtg atccgcgcgt tcaatgataa tggtgttggc ggtaggaatg tcgatcccgg
     2341 tttcgataat ggtggtacaa accagcacgt tgaaacgttg atgatggaaa tcattcatca
     2401 cccgttccag ttcgcgctca cgcatttgcc cgtgaccgat ggcaatccgc gcttcaggta
     2461 ccagttctgc cagccgttcg gcggctttct ggatgttttc cacatcattg tagagataat
     2521 aaacctgtcc tccgcgcaaa atttcacgca ggatcgcctc ccggaccacc agactgtcat
     2581 actcacggac aaaggtttta actgccagac gacgggcggg cggcgtggcg ataatcgaca
     2641 gatcgcgcat tccgctcatt gccatattca gcgtacgtgg gatcggcgtt gcggtaagcg
     2701 tcagaatatc cacgttcgcg cgcatcgctt taatgcgctc tttatgacgc accccgaagc
     2761 ggtgttcttc atcgacaatc agcaggccta aatctttaaa cttgacgtca ctttgcagca
     2821 gtttgtgcgt accgatcaga atatcgattt tcccttccgc cacttccgca aggatttgcg
     2881 tctgctcttt ggcgctgcgg aaacgggaga tcatttcgat acgtaccggc cagttggcga
     2941 aacggtcgcg gaagttgtcg taatgctgct gcgcgagaag ggtggtaggc accagcaccg
     3001 ccacctgttt gtggttatct accgccagga aagcggcgcg catcgccact tctgttttac
     3061 caaagccaac atcgccgcac accagacgat ccattgccag cggctgacac atgtcgctaa
     3121 gcaccgcatt aatggcttga gcctgatccg gcgtggtttc aaacgggaag ctgtcgcaga
     3181 ataactgata ctgctcacga tcgtgtttaa acgcgaagcc ctctttggcg gcgcgttgcg
     3241 cgtagatatc cagcaattcc gccgccacat cacgcacttt ttccgccgct ttctgccgtg
     3301 cgcgtgacca ggcatcgcca ccaagtttat gcagtggtgc gttttcttct gcgccacccg
     3361 cgtaacggct aatcagatgc agtgacgaca ccggaacata cagtttggcg tcgttggcat
     3421 aggtgagcat caaatactcg ccagtaatgc ctccagcttc gagcgtggtc attccggcat
     3481 aacgaccgac gccgtgctcc agatggacca ccggctgacc aatatgcagc tccgcaaggt
     3541 tacggatcag tgtatcgggg ttgatggcgc ggcgagaatc ctgacgacga cgggcaacgc
     3601 gttcaccgag cagatcgctt tcgcaaatca gcgccaggtt acgcatcgta tcgacaaaac
     3661 catgttcggc agcgccaatc atcagataac gcccacggtc gctggcttca tcaagacgca
     3721 taatgcgttg cggagcaatt ttaattcgcg cgagcagttc acccagcgct tcacggcgac
     3781 cttcactttc taccgagaac accaccggac cgtcgaaagt ctcgaggaac ttacgcagcg
     3841 catccagcgg cgctttttgt tgtgcctgaa tggccaggtc tggcagtttc tggaaaccta
     3901 aattggcatt cgctgctttt gtcggtaaat gttcagtttt tagctgcaca cgcggccagt
     3961 ttttcagctc tgaaaagagc tcgtctaccc gcagccagag cgattgtggt ggcaacagtg
     4021 gacgcatcgg atcgacgccg cgattttcaa aacgcgccag cgtgtcagcc tggaaacgtt
     4081 cggcactgtt ttccagatcg ccagtattta tcagcagcgt attggcaggg aaataactga
     4141 acagtggcgg cagcggttcg ctgaagaata gcggctgcca gtactcgatc ccggcaggta
     4201 atgtgccttt actcacttgc tggtaaatat gttccggatc gcgcttcact tcgaaggtat
     4261 cgcgccactg gctgcggaac agttcaattg ccgctttatc ggtcggaaat tcgtgcgcgg
     4321 gcagcagatt gatctcttct acttcctcca gcgtgcgctg gctatcgacg tcaaacaccc
     4381 gcaggctgtc gatttcatca tcaaagaaat caagacgata aggcagctca ctccccatcg
     4441 ggaagagatc cagcaacgcg ccgcgcgtgg cgtattcgcc gtgctccatc acctggtcaa
     4501 catggcgata accggcgctg tccagttggg ttcgtaatgc atctcgtgac aggcgctgac
     4561 cttttttcat caccagcgca tgaccgtgga gaaagttgtg cgggcaaacg cgctgcataa
     4621 gcgtattcac cggaacaatc agtacgccac gctgcatggt cggtagctgg taaagggtgg
     4681 aaaggcgcga ggagataatg tcctgatgag gcgaaaaact gtcgtaaggg agggtttccc
     4741 agtccgccag attcatcacc atctgatcgg tgaactggct gatttcatca tgcaaacgca
     4801 gagcattttg catatctggt gcaatgagta ccaccggacc ggcgtgacgt tcggcaattt
     4861 ccgctaccag cgtcgcgcag gcagcgccgg ttaactcgcc cagcagacgc tgctcacccg
     4921 ctttgacggg cagcgtataa cgatattgtt caggcataac ggttgtcaga ttctcgttag
     4981 gatatgcctc aacatatggg ggcatatctc tgataagcaa tgctgttcat tatccgtaat
     5041 cgacatggtt tagcaaattg aatcgccccg gctggggcga tggcttagcg ggagtaactg
     5101 acggccgcgg gcagagaagg cggggagaac aacagatcgc tgaatgcgcg ctgtgaaagt
     5161 tttcttacca gcaagcccgc aacagtacag atgaacaaac taacaaaggg gtaaaccagc
     5221 aggagtgtca gttcgacttg cggcgaccag cgtgctgcgt tcatttgcgc aagcagagtt
     5281 aagctgaata tttcaaccag aatgcgatgg gtggtgtaga tagcaatggt gttggaacca
     5341 atcacattca gcaggctgga ggaacgcata ccgaaacgct gctcgtactg ataaaacagc
     5401 ttcatgatta ccacaatcga taccagcgac aacagcagcg agatagtaaa caaccaggca
     5461 ccgaccgcca gaacggtcag caaagaagcc atcagcagat ggcggcgcaa cggcacctct
     5521 ttaacacagg tcattattgt tgcgccaaac catgcgccaa ggctgtaata aggcaaattg
     5581 cggatcacac tgttcattcc ccaccacggc gtgggaacga aattaaccgc cacactcatc
     5641 agtacaaaca gaacaaataa cggcagggcc aggcggttaa aaattttaca tatcacgaaa
     5701 tagacaatta aagcatacag ataccacaag ctggtgctgg cggtgatcat cccgtgcagg
     5761 aactcaccgg tagaatcggc ataagcggca ttggaggcat tacttaaatt gcgctcaggt
     5821 gccagccact gatttagtgc acttatcgcc agccactgca ctacgcccca aagtgccagc
     5881 acccagaaga tgttccagat gcgtttatcg agacaatttc cccacggtac gctgtcgata
     5941 tagcggcgaa tcaaatagcc agagataaag aaaaaaaccg gcatacgaaa gggtgcaagg
     6001 taaagattga aatagatcca gcatttgctc aggacttccg ataacggatg ctgaaaagtg
     6061 gtcagatgcg gataaaaggt aatgaccgag tgataaatca ctaccagaca aatacataac
     6121 cctttgatct ggttaatcca tagctctttt tgtttcatca ggacaacacg ctctttttat
     6181 ttaacatgct gttaatcctg cggggcgagt tgcgtggtgt aatgcgtatc tgtctgtatt
     6241 tggttatttt tcagataaag atcagcccac aaggccgatg agaaaatgag gccctttagc
     6301 cgattgattt acgggggctt ttcagattag ccctgacgat cacttacagt tcagacgttt
     6361 acccatcccg ctttcgctta tatactcgtg tctttgctac agcaaccaga cggatttcat
     6421 gtaccaacct gtcgctctat ttattggcct gcgttacatg cgtgggcgtg cagcggatcg
     6481 cttcggtcgt ttcgtctcct ggctttctac catcggcatt accctcgggg tgatggcgct
     6541 ggtcacagta ttgtcagtga tgaacggctt tgagcgcgag ctgcaaaaca acatccttgg
     6601 cctgatgcca caggcaattc tctcttctga gcatggctct cttaacccgc agcaactccc
     6661 ggaaacggca gtcaaactgg acggcgttaa tcgcgtcgca cctattacta ccggtgatgt
     6721 ggtactgcaa agcgcgcgca gcgtggcggt cggggtgatg ctgggtatcg acccggcgca
     6781 aaaagatcca ctaacgccgt atctggtcaa tgtgaaacaa actgacctcg agccggggaa
     6841 atataatgtc atcctcggtg aacagcttgc ctcacagttg ggcgtcaatc gcggtgatca
     6901 aatccgcgtg atggtgccat ctgctagcca gttcacgccg atggggcgta ttccaagtca
     6961 gcgcctgttc aatgtgattg gcactttcgc cgccaacagt gaagtcgatg gctatgaaat
     7021 gctggtgaat attgaggatg cctcgcgtct gatgcgttat ccggcaggca atattaccgg
     7081 ctggcgtttg tggctggatg agccgctgaa agtcgactca ttaagtcagc aaaaactgcc
     7141 tgaaggcagc aaatggcagg actggcgtga ccgtaaaggc gagctgttcc aggccgtacg
     7201 catggaaaaa aatatgatgg gcttgctgct gagcctgatt gtcgccgttg cggcatttaa
     7261 cattattacc tcgctgggac tgatggtgat ggagaagcag ggcgaagtgg ccattcttca
     7321 gactcagggg ctgactccgc gtcaaatcat gatggtcttt atggtgcaag gggccagcgc
     7381 cgggattatc ggtgcgatcc tcggagcggc gcttggcgca ctgcttgcca gccagttgaa
     7441 taatctgatg ccgataatcg gcgtcctgct tgatggtgcg gcgctgccgg tcgctatcga
     7501 gcctttacag gtcattgtta ttgcgctggt ggcgatggct atcgcgctgc tgtctacgct
     7561 ttacccttcc tggcgcgctg ccgccactca acccgctgag gctttacgtt atgaataaga
     7621 tcctgttgca atgcgacaac ctgtgcaaac gctatcagga aggcagtgtg caaaccgatg
     7681 tactgcacaa tgtcagtttc agcgtgggcg aaggtgaaat gatggcgatc gtcggcagct
     7741 ctggttccgg taaaagcacc ttgctgcacc tgttaggcgg gctggatacg ccaacctccg
     7801 gcgatgtgct ctttaacggt caaccgatga gcaaactgtc gtcggcggcc aaagcggaac
     7861 tgcgtaacca gaagctggga tttatttatc agtttcacca cctgttgccg gattttaccg
     7921 ccctggaaaa cgtggctatg ccgctgttga ttggcaagaa aaagcccgct gaaatcaaca
     7981 gccgtgcgct ggaaatgtta aaagcggtag ggctggagca tcgtgcgaat catcgcccat
     8041 ctgaactttc tggcggcgaa cgccagcgtg tggcgattgc ccgtgcgctg gtcaataacc
     8101 cgcggctggt actggcggat gaacctaccg gtaacctcga tgcacgtaac gccgacagca
     8161 tcttccagtt gcttggggaa ttgaatcgct tgcagggcac cgccttcctg gtggttactc
     8221 acgacctgca actggcgaaa cgtatgagcc gccaactgga aatgcgtgat ggacgtctga
     8281 cggcggaact gagcctgatg ggggcggagt aatggcgatg cctttatcgt tattgattgg
     8341 cctgcgtttt agccgcggac ggcgacgcgg cggcatggtg tcgttgatct ccgtcatttc
     8401 taccattggc attgccctcg gcgtggcggt attgatcgtc ggcttaagcg cgatgaacgg
     8461 ctttgaacgc gaactgaata accgcattct ggcggtagta ccgcacggtg aaattgaggc
     8521 ggtagatcag ccgtggacca actggcagga agcactggat aacgtgcaga aagtgccggg
     8581 tattgccgcc gctgcgccgt atatcaattt caccggactg gtagaaagtg gtgccaatct
     8641 gcgcgcaatc caggtgaagg gcgttaaccc gcaacaggaa cagcgtctga gcgcattacc
     8701 ctcgtttgtc cagggcgatg cgtggcgcaa ttttaaagcg ggcgaacagc aaattatcat
     8761 cggcaaaggc gtggcggatg cgctgaaagt gaagcagggg gattgggtgt cgattatgat
     8821 ccccaactcg aatcctgagc ataaactgat gcagccaaaa cgtgtgcgtt tgcacgttgc
     8881 cggtattttg cagttgagtg gccaactcga tcacagtttt gccatgatcc cgctggccga
     8941 tgctcagcaa tatcttgata tgggttccag cgtgtcaggt attgccctta aaatgacgga
     9001 tgttttcaac gccaataagc tggtacgcga tgcgggggaa gtgaccaaca gctatgttta
     9061 tattaaaagc tggattggta cttacggcta tatgtatcgc gatatccaga tgatccgcgc
     9121 cattatgtat ctggcgatgg tactggtgat tggtgtggcc tgtttcaaca tcgtctccac
     9181 cttagtgatg gcggtgaaag acaagagtgg cgatatcgca gtattaagaa cgctgggggc
     9241 gaaagatggt ttaattcgcg ccatctttgt ctggtatgga ttgctggcag ggctgttcgg
     9301 cagcctgtgt ggtgtgatta tcggcgtagt tgtttcactg caacttaccc cgattattga
     9361 gtggattgaa aagctgatcg gtcatcagtt cctctccagc gatatctatt ttattgactt
     9421 cttgccatcg gaattgcact ggctggacgt cttctacgta ctggtcacag cattgttgct
     9481 gagtcttttg gcaagctggt atccggcgcg gcgcgccagt aatattgacc ctgcgcgagt
     9541 ccttagcggc cagtaaaggc agtacattaa aacaaggagc ggcaatgtat tacgggtttg
     9601 atattggtgg aacaaaaatt gcgctaggcg tgtttgatag cggtcggcag ttgcagtggg
     9661 aaaagcgggt gccgacaccg cgtgacagct atgacgcatt tttagatgca gtgtgcgagc
     9721 tggtagccga agctgaccgg cgttttggct gtaaaggttc tgtcggcatc ggtattccgg
     9781 gaatgccgga aacagaagat ggtacgctgt atgccgccaa tgtccctgct gccagcggta
     9841 aaccgctgcg tgccgacctg agcgcacgtc ttgatcgcga tgtacgcctt gataacgatg
     9901 ccaactgctt tgccctttct gaagcatggg atgatgaatt tactcaatat ccactggtga
     9961 tggggttgat tctcggcacc ggcgttggcg gcgggctgat tttcaacggt aaacccatta
    10021 ccggtaaaag ctatattacc ggcgagtttg gccatatgcg tctgccggtt gatgcgttaa
    10081 ccatgatggg gctggatttc ccgttacgcc gctgcggctg tggtcagcat ggctgcattg
    10141 aaaattatct gtctggtcgc ggttttgcgt ggctgtatca acactattat catcaaccgt
    10201 tgcaggctcc tgagatcatt gcgctttatg atcaaggcga tgagcaggca agggcgcacg
    10261 ttgagcgtta tctggattta ttagcggttt gtctgggaaa tatcctgacc attgttgacc
    10321 ctgacctggt cgtcattggt ggtggcttat cgaatttccc ggcaatcaca acgcaactgg
    10381 cggaaaggtt gcctcgtcat ctcttacctg tagctcgtgt tccgcgcatt gaacgcgcgc
    10441 gccacggtga tgcgggggga atgcgtggtg cggccttcct acatctaacc gattaatcaa
    10501 cagaggttgc tatgctgtcg cgtcggggtc atcggttaag tcgttttcgt aaaaataaac
    10561 gccgcctgcg cgagcgtttg cgtcagcgta tttttttcag agataaagtg gtgccggaag
    10621 caatggaaaa accaagagta ctcgtactga caggggctgg gatttctgcg gaatcaggta
    10681 ttcgtacctt tcgcgccgca gatggcctgt gggaagaaca tcgggttgaa gatgtggcaa
    10741 cgccggaagg ttttgatcgc gatcctgaac tggtgcaaac gttttataac gcccgtcgtc
    10801 gacagttgca gcagccagaa attcagccta acgctgcaca tcttgcgctg gcgaaactgc
    10861 aagatgctct cggcgatcgc tttttgctgg tgacgcagaa tatcgacaat ctgcatgaac
    10921 gcgcaggcaa taccaatgtg attcatatgc atggggaact gctgaaagta tgttgttcgc
    10981 aaagtggcca ggtcctcgac tggaccggag atgttacgcc agaagataaa tgccattgtt
    11041 gccagttccc ggcacccttg cgcccgcacg tagtgtggtt tggcgaaatg ccactcggca
    11101 tggatgaaat ttatatggcg ttgtcgatgg ccgatatttt cattgccatt ggtacttcgg
    11161 ggcatgttta tccggcggct gggtttgttc acgaagcgaa actgcatggc gcgcacaccg
    11221 tggaactgaa tcttgaaccg agtcaggttg gtaatgaatt tgccgagaaa tattacggtc
    11281 cggcaagcca ggtggtgcca gagtttgttg aaaagttgct gaagggatta taaagcggga
    11341 aaaattgcct gatgcgctac gcttttcagg cctgtgaagg ggcgcaatta attgaatttg
    11401 cgggttttgt gggccggata aggcgttcgc gccgcatccg gcataataca tgcgaacttt
    11461 actgcctctt ttaccgttta ctcgtccata gtaacgcggc aataaacgta cagacaccaa
    11521 tcagccctgc ccagagtggc gatattctgg taatcgcaaa gctgttatcg atatacaaac
    11581 ataaagcctg ataacccata ttggcaacag ccatcgccac tgcaaatttt aacgcataat
    11641 aaaggcgcgt tgtgtagtta aaaaatgcag caaagacgat gaatgcaact gcccaggcaa
    11701 aaaggcctaa tgtcgtggta ctgccgggtg gatcattttc atgctgttgt ccgggatgcg
    11761 gataatatgt cagtggtggc gtcggttgtt ttaatatctg tgcttgtaaa acagccaatg
    11821 cattattgat accctgatac cagagatccg catgtaaata agccagcgtg tgattttcta
    11881 ccagaaggcc gagatgttta tcgtccatac tgctttcgac ctggccgttg gttatcataa
    11941 ttaaccgtgg ctcgcgggta gtaaagagta acagaacact tccttttaca ttactcattg
    12001 aagatgccgc atgcgccagc atcgctttgc cattttgcct gagactggct tcattattaa
    12061 tgtcgggtaa aaccacaata aacggtgcaa ctccggtttg ttggcgaaaa agttcggctt
    12121 tatgcgcgat ctcttcagca tctggtacag taccggtaac atccgtaaaa agtgtcttca
    12181 ttattggaat taagggctta ctgattgccg tcagaggcag gaacatcagt aaactgagca
    12241 gtatcatgat ttttttcatg ggcgggactt ccgtttcctg cgtgtctttt ttgaggaggg
    12301 catactgatt aataaaccga cggcgataaa aataacgccc ataatgccga taaaccccca
    12361 tttaaaaaaa ttaacccacg ggttatttat ctcagccttt tcaggatgtc ctggagaata
    12421 ataaacatta actttatcgc cttcgctgtc ttcataaaaa tcgctgccaa tactcgaatt
    12481 gaaaattagc gtgtaatctg gcgtcggacg aaaggcaacg acgggatacc acgaaccatt
    12541 tttacccgtg cgatgagaat gactggagtg ccagacggta tcaacaatga cgccttcagt
    12601 atgaatcgcg tctttgctat aggtgaactg gttataaaag ataacaacag aaatgaggat
    12661 cagtaccaga ccgataccga agaatatacg aaagaaaact ctactgtcct ggctcattag
    12721 ccacatcctt gctaaataaa aaacgggcgg taataccacc gcccacttgc tgaattaacg
    12781 tcctgctttc agcttctgat aatactcttc ataaatgctg ctggcggagc ctacgtcatt
    12841 ttgccattca ccatttttaa tggtttcagc atccgggtag agcgttttat cgttcgccac
    12901 ttccggactt aacagcttac gcgccgcaag gtttggcgtt ggataaccga tagtttcagc
    12961 aacctgtttt gccacatccg ggcgcagcag gaagttgatc aatttcagcg cgccttcttt
    13021 gtttttggca tttgccggga tcgccaggct gtccatccag aaaatgccgc cttctttcgg
    13081 ccataccaca tcaattggcg tacccgcctg gcgtgcaacg aaagcagaac cgttccagat
    13141 catgccgagg ttaacttcgc cttccatata cgggttagcg gggttatcgg agttaaacgc
    13201 cgcgacgttt ggcatcagtt ttttcagctc gttgtatgca gcttcaatct ctttcggatc
    13261 ggtggtgtta ccggagtagc ccagcttacg cagcgccatc tggaacactt cacgggcatc
    13321 gtcggtcagc agcaggctgc ctttgtactc aggcttccac agatcggccc agctggtgac
    13381 agatttcgga tccaccgcat caccgttaac gccaatcgcc gttgcacccc agatatacgg
    13441 aatggaatag tcgttattcg ggtcaaacgg cttgttgagc atgtctggat cgaggttgct
    13501 gaaattgctt aacttcgact tgtcgatctt ctggatcatc ccttctttac gcattttatc
    13561 gacgtaatag gttgaaggaa ccaccaggtc ataggcaccg tctttgtacg ttttcagctt
    13621 cgcgtacatg gtttcgttcg actcgtaagt cgaatagata accttaatac cggtttcttt
    13681 ggtgaactgt tcaagcagtc ctggcggcac gtactcggtc cagttgtaga aatacagcgt
    13741 gttgttgtca tcggcgtgag cggcgctcat gcccagtgcc agagcacccg ccgcgagcag
    13801 gtggcgtgac cattttttca tttaacgtcc cctgtgttac ctttcgtttt atcacgagca
    13861 ataagctggc tggcaattac catcaccagc gacagcacca gtaatatggt tgccagcgcg
    13921 ttaacttccg gcgatacgcc gactttgacc atcgaataaa tttttaacgg cagaatttca
    13981 taactcggcc cggtgacaaa cgaagaaacc accacgtcgt ccatcgacag ggtaaagctt
    14041 aacacccagc ccgccgctac cgctggcatt gccagaggca gaatgatttt tcgcagaatg
    14101 gtaaattcgc tggcaccgag atctttcgcc gcttccagca ttcgcacgtc aaaacctttc
    14161 aggcgcgaat acactgtcac caccacaaat ggcaggcaga aggtgatatg cgagaacagc
    14221 agcgaccaga agccaagctg aatacccagc agcataaaca gcaccagcag cgaaatcgcc
    14281 atcacgatat ctggtgacat catcaccaca aacagcattc cgctaacgaa cggcttacca
    14341 cgaaagcgat aacggtacag cgcaactgcc gtcagtgaac cgataagcgt agcaaacgtc
    14401 gccgaaaaca ccgccattgt tagtgaatgc tgcgctgcct gtaacaggct gtcgttgttc
    14461 atcagcaggc tataccattt ggtggtaaaa ccctgccagt tgatgccaaa gcgcgagctg
    14521 ttaaaggagt tcacaatcaa aataatgatt gggatataca ggtacgcgta gatagcggtc
    14581 ataaaaccgc cgcgaagcag tcgaccgatc attcgagttc caccttctta ttcataaggt
    14641 ggagcacttt caggattttg aatttattca attttttcct ttctccaaaa tctctccaaa
    14701 acgtttcccc aaaactaggc cacgcatttt acaacacttt tttcctgaga tggtgacgga
    14761 tattccatat ggtgtttgtc aaataaccaa cttaacccat tcctgacctc gagtatcgtt
    14821 atagcgatcg gtggttgcct ggactttatg tcctagtaat gtttttgtat cgataccctg
    14881 tgcacggtac agccgttctg atagagagcg ttgttcatga aatgttggcg gagtttttcc
    14941 tgctggtgga attatcccag ccagatcccg tgctttggca aagtagtcgc tcaggttgtc
    15001 tttactcatc ggcttcggtt gtttctggtg ccgactatgg attagatatg gacttaatat
    15061 tctgtctcgg cacccatcaa taacttcttt taacgttatc ccaatggcat cacagcgtag
    15121 tgtaagcggt aacgccagac gcattccggt ttttccctgg gtgatatgca agtgttcgtt
    15181 ccacacatct gaaaaacgca tgtggcaaat gtcatcacgg cgctgaccag taacaatcgc
    15241 aagaagcatt gcgttacgga taaagtgttt ttcaggcgtt gcattgtaaa ttttttgcca
    15301 gtcttccatg gtgagcctgg ctctggttac tttagggatc ggtttacggg tagcctccgg
    15361 aggattccat ccaggaggaa cttcccctgc atgctgtgct tctttataaa tatcaaccca
    15421 taatccacga tttactctcg ctgtgctgac catgtcttta tccagccact catccagtat
    15481 taatgcaaag tctcttactt ccagttcttt caatgggtgg tttcccagac gggaaaccag
    15541 gtatgcagcc attcgagttt tttctttgtg agttgtagct gcaatatctc catttttcag
    15601 tcgcgtgtcc tgtattttca gatatcgatc aacccatgcc tttaatctga taccccgacg
    15661 ttttgttgct gacggacttt catcaatttt gcgcatgaaa tattcagctt ctgctgcagc
    15721 tattcgctga ttggctgtgg aagcgatttt ttctgcctta cctttgtctg ttccgagtcc
    15781 gtgaaatttt ccagtcacag gatttttata ctggtagtaa actctgccag ttctgcgatc
    15841 aaacttttcg taaagaccgg ctacgtcagt gctgtttttt cgtggcctcg gtgacatgag
    15901 ttaaaatctc cttcagtgca tcatcatcgc cagtatgaat ttccggcgca attcccgttt
    15961 caccaggccc aacaaatact gctcggcgat ctatcagcca acgcccacga attttttgtg
    16021 gccttggaac gatgtatcct agttttccgt atttcaccag ggtagtgttt gttattggga
    16081 gactgaaccg ttttggtttc cactcgtcga gcgttatcag gtactgttcg ctcatggcta
    16141 tcactccgga acgcgccagt tgcagaatat caacgacaac tggcgacggt tgaacattaa
    16201 aaatcagcct ga
//
LOCUS       DAZFRE010000102        15860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFRE010000102 DAZFRE010000000
VERSION     DAZFRE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.102"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHKUI_003987"
     CDS             complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHKUI_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6600081.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQ"
     gene            complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHKUI_003988"
     CDS             complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHKUI_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6600082.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(2576..2905)
                     /gene="ybaB"
                     /locus_tag="ACHKUI_003989"
     CDS             complement(2576..2905)
                     /gene="ybaB"
                     /locus_tag="ACHKUI_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6600083.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(2958..4889)
                     /gene="dnaX"
                     /locus_tag="ACHKUI_003990"
     CDS             complement(2958..4889)
                     /gene="dnaX"
                     /locus_tag="ACHKUI_003990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6600084.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(3561..3625)
                     /gene="dnaX"
                     /locus_tag="ACHKUI_003990"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(5018..5569)
                     /gene="apt"
                     /locus_tag="ACHKUI_003991"
     CDS             complement(5018..5569)
                     /gene="apt"
                     /locus_tag="ACHKUI_003991"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6600085.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(5722..6099)
                     /gene="ybaN"
                     /locus_tag="ACHKUI_003992"
     CDS             complement(5722..6099)
                     /gene="ybaN"
                     /locus_tag="ACHKUI_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6600086.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            6169..6696
                     /gene="priC"
                     /locus_tag="ACHKUI_003993"
     CDS             6169..6696
                     /gene="priC"
                     /locus_tag="ACHKUI_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6600087.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            6710..6871
                     /gene="rsmS"
                     /locus_tag="ACHKUI_003994"
     CDS             6710..6871
                     /gene="rsmS"
                     /locus_tag="ACHKUI_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6600088.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(6924..7022)
                     /locus_tag="ACHKUI_003995"
                     /pseudo
     CDS             complement(6924..7022)
                     /locus_tag="ACHKUI_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7083..10445)
                     /gene="mscK"
                     /locus_tag="ACHKUI_003996"
     CDS             complement(7083..10445)
                     /gene="mscK"
                     /locus_tag="ACHKUI_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6600089.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(10573..11220)
                     /gene="acrR"
                     /locus_tag="ACHKUI_003997"
     CDS             complement(10573..11220)
                     /gene="acrR"
                     /locus_tag="ACHKUI_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6600090.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11362..12555
                     /gene="acrA"
                     /locus_tag="ACHKUI_003998"
     CDS             11362..12555
                     /gene="acrA"
                     /locus_tag="ACHKUI_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6600091.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETSRINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            12578..15727
                     /gene="acrB"
                     /locus_tag="ACHKUI_003999"
     CDS             12578..15727
                     /gene="acrB"
                     /locus_tag="ACHKUI_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6600092.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         3669 a         4197 c         4185 g         3809 t
ORIGIN      
        1 gctggttcat acgacgaata aacaggttcg gatcttccag cgtaccgcgt tctgccagca
       61 gcgcctgatc cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatccg
      121 ccgcacgttt caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca
      181 ctttctggcc cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcgtcgg
      241 tcgaaacgat cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct
      301 cgccgagcag ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg
      361 cgctctcatc aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact
      421 ggaacggttt accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg
      481 aaagcagcag aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg
      541 ctgccgcata gctgtctgcg gtgatgtagt agattttctc ctgcccttct ttcatgcggg
      601 aaacgtagtc ttccagagat acggtctgcg cagaagaatc ggtatgggta gaagcaaaac
      661 gcagcaattt ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta
      721 ccaggccaaa ctgttgccag aaggtctggt atttttccgc gtcgtctttc gccagttttt
      781 ccagcatttg cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt
      841 cctggaggat ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca
      901 cgaagcgcag atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgga
      961 cgtacagttt caggccgtgt ttatgatcgc ggttccacat atcccacgga gcctgggacg
     1021 gaatgtacag caggctggtg tactcctgct taccttcaac gcggttgtgg ctccaggtca
     1081 gcggatcgtt aaagtcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga
     1141 tttccgactt gttacgagtc cacagcgcct gcgctttgtt gattttctcc caggagataa
     1201 tggtttcgcc gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg
     1261 aatatttgct gatgatggaa cgcacacgcc agtcatcgag gaactcgtct tcgccttcac
     1321 gcagatgcag ggtgatttca gtaccacgat cttctttagt gatgtcggca acggtgtatt
     1381 caccttcgcc agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag
     1441 tacgcacggt cactttgtca gccacgataa acgcagagta gaaaccaaca ccaaactgac
     1501 cgatcagctg gctgtctttc gcctggtcag agcccaggga ttcgaggaat gatttggtgc
     1561 cggatttagc gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt
     1621 tatcggagat ggtcagcgtg cgcttgtctt tatcgaaaga gacacgaacg cgtagttcac
     1681 catcaccttc gtacaggtcc gggttagaaa gcgcgcggaa acgcagcttg tccgccgcat
     1741 cggaggcgtt agagataagc tcacgcagga agatttcttt attggaatag agagaatgga
     1801 tcatcaggtg cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca
     1861 tgtaggtcta cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg
     1921 acaagggaga ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga
     1981 atcttatgac gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagttcg
     2041 ccgccaaccg gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcacaaagc
     2101 tcggcaatgt agttagcggt agcttcacct tcaaccgtgg ggttggtggc gaggatcact
     2161 tcagtgattt tttcctctgc cagacgctgt tccagacgat caagcccgat atcatcagga
     2221 ccgatgccgt ccagcggtga caggtgcccc atcaacacaa aataacggcc tgaaaactgc
     2281 cccgtctgct caatggcgta gatgtccgcc ggactctcca ccacgcagat ttgaccgttt
     2341 tcctgacgac gcggattcga acagatgtta cagacttcct gttcggtgaa ggtgcggcaa
     2401 tcggcgcagt gaccgatttc cgacatcgcc cgggtgagcg cctgcgccag acgcatcccg
     2461 ccgctacgat cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg
     2521 cccggcagac agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga
     2581 acggcatctt aaagccaggc ggcagctgca ttccggagga tacagaggcc attttttctt
     2641 tctgcgtttc ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca
     2701 gcatctcttt gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttgtgtg
     2761 caccgttgat ggtcactttt accagacctg cgccagattc gccggtgact tccagctgcg
     2821 cgatctcttc ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt
     2881 tacccagacc gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac
     2941 gtaagctgtg cttacgatca aatggggcgg atactttctt catccagctc cgcatcgaag
     3001 aatcgacgca gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt
     3061 ttttcttcgt atatcgcctg acgccattcc agcggcgtac gcaccgcggg attatcatct
     3121 tcaacgatag tcagttcaac cgttgaacct tttaacgtgc tcaacgcttc agccagtttt
     3181 tgctgtgcgc cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg
     3241 ttgtcgctct cctctttcca ggcatttaac gccacctgtt cgaccagttt tggtagcgaa
     3301 agttgactca cctgagccgc ccacgggtcg cgctcaatgg cttctgccgc cagcttcgcc
     3361 gccagttccg gcgttttttc atgttccagc gcttttttca gcgccttcgg cgtggcgacc
     3421 acttcttttt gctgcatcac tggagtggtc gccttccagc gatacgcttc ttttttggct
     3481 ggcgcttttt ccagcgccga tggcaccggg cgcgcctgaa cgcgatcggt gaccgaagcc
     3541 agtctttcca gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cggttcactc
     3601 ttttttgctt tggttgctcc ctgcacgcgc tgcaactgct ggcgcgccgc cagcacctgg
     3661 ctggtggttt ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggttgtggc
     3721 ggcacctggg ttggcgtcat tactgccgtt ggcgcaacgg gcgcaaagga ctggcgtggc
     3781 acttctggct caggcagagg catacgcgga tggaacgcca gcgcgcgcag cagcgtcatc
     3841 tcaacaccca tacggcggtc tggcgcatac ggtaattctt tgcgaccaat caacagcgtc
     3901 tgatagtaaa gctgaatatc cgtcggcggt atggtgcgcg ccagttcacg catccgcagc
     3961 tcaatggcgg ccatgtcgtt gccaagagca gcaggcgaaa gttgtaccat cgcaatacga
     4021 tgcaacagac cgagcatttc caccagcaac gcttcccact cgataccacg ggcagcagct
     4081 tcattaatta gcgccattac gcgctcgccg ttggcctcga ccatcgcttc aaccagcgat
     4141 aacgcctgat cgtcgtcaag cgtacccagc atcgcactga cggcctgggt tgaaacctgg
     4201 ccgtcaccgc tggcaatcgc ctggtcggtc agacttaagg catctcgcag gctgccttca
     4261 gcggcgcgtg ccaacaattg cagcgcccgc ggctcgtgag cgatatgttc ttcgttgagg
     4321 atgtgctcaa gctgatggcg aatttgctcg acatccagcg ccttgagatg aaattgcaga
     4381 cagcgtgaca aaatcgtcac cggcaatttc tgcggatcgg tcgtcgccag caaaaactta
     4441 acgtgctctg gcggctcttc aagggttttt aacagtgcgt taaagctgtg acgcgacagc
     4501 atatgcactt cgtcgatgag ataaactttg aaacgaccac gcgccggagc gtactgaacg
     4561 ttatccagca ggtcgcgggt atcttcgact ttagtgcgcg aggcggcgtc gatttcaatc
     4621 agatcgacaa agcgcccctg ctcgatttca cgacagttat cgcacacgcc gcacggcgtc
     4681 gcggtaatgc cggtttcgca gtttagcccc ttcgccaaca gtcgggcgat agaggttttt
     4741 ccgacgccac gggtgccgga aaaaagataa gcatgatgaa tacgccctaa cgacaagccg
     4801 ttcgccagtg cggtcagcac atgttcctgg ccgacgacgt cagcaaaggt ttgtgggcgc
     4861 cattttcggg ctaagacctg ataactcatt ggcaggctct gaaacgctgg aaggtggatt
     4921 cacgaagggg taatgctaac acagccccgt cagaacggcg aggctgaatg cacatcttgt
     4981 gctgcccgta gcgtgagcag cgcaagactg acgataatta atggcccggg aacgggacaa
     5041 ggctgtagct ggtaatgccc tgtttttcga gacgctgttc gccgccgaga tcgaacaggt
     5101 tgataatgaa cgcagcgtca gctacttcac cacccagacg acggatcagt ttaacggtcg
     5161 cttcgatagt gccgccggtt gccagcagat cgtccaccac cagaactttg tcgcccggtt
     5221 tgatggcatc aacgtggatt tccagctgat cggtgccgta ttccaggtcg taagtttcac
     5281 tgatggtttc acgcggcagt ttgcccggtt tacggaccgg tacaaagcca acgcccagac
     5341 ccagagctac cggagcgcca aacaagaagc cacgcgcttc ggtgccgacc actttggtaa
     5401 tgcccgcatt tttgtaacgc tcaaccagca agtcgatgct gagagcgtaa gctttcgggt
     5461 cttccagtaa gctggtgaca tcgcggaaaa gaatgccggg ttttgggtag tcctgaatgc
     5521 ttttgatgct atttttgaga tactcaagct gctgtgcagt cgcggtcata agtgtatgcc
     5581 tgcttgttac ggtggtactc acggcgcgtt ttaaacgtat caaaagtaac agttgtttaa
     5641 cctttgaccg cctgcgcctg tgctcgaaaa cgccagaatg tactggctgt acgcaataat
     5701 tgcaactgcg attgttgtgc ttcagtgctt ttcttgcttt tcatcaatca ccggaattcg
     5761 ccacatataa aaaagcaaac aggcgagaat taccagcaac atgatgcgca cccacggcat
     5821 ctggacgaac cacagcgaaa tcgtaaacgt aatcaaaata agcaaaatcg cccgtggttt
     5881 tgcgccgcgc ggcatcgcat gatgtttctg ccagaaacgt agatagctgc caaaccatga
     5941 gcggtacagc aaccaggcgt gaaagcgcgg ggaagaacgg gcaaagcacc aggccgccag
     6001 cagaataaac ggcgtcgtcg gtaataccgg taataccacg cccagcgtac ccagcactac
     6061 cgccagccag ccaatgatga ttaaaatgat tcgttgcata ttgagtgttg tcagcttaca
     6121 gagtggctac tttagcataa caattatcat tttcattgag gtcttatcgt gaaaaccgcc
     6181 ctgctgctgg aaaaactgga aggacagctc gccacgctgc gtcagcgttg tgccccggta
     6241 gcgcaatttg ccacgctaag tgcccgtttt gacaggcacc tttttcagac ccgtgcgaca
     6301 acactacagg cttgtctcga tgaggcgggc gataacctgg ctgcgcttcg tcatgcggtt
     6361 gagcagcaac agctgccgca agtcgcctgg ctggcagaac atctggcggc gcaactggag
     6421 gctatagcgc gtgaagccac agcctggtcg ctacgcgagt gggatagcgc accaccgaaa
     6481 attgcccgct ggcagcgtaa acgtattcag catcaggatt ttgagcggcg gctacgtgag
     6541 atggttgccg aacgtagagc ccgtctggca cgagcgaccg atctcgtgga acagcagacg
     6601 ctgcatcgtg aagtggaagc ctatgaagcg cgcctggcac gctgccgcca tgcgctggaa
     6661 aaaatcgaaa acaggttagc gcgtttaacc cgctagcaat ggagagagta tgtcactgga
     6721 aaatgcccct gacgatgtca aactggccgt cgatttgatt gtgctgctgg aagaaaatca
     6781 gatcccagcc cgcaccgtgc tgcgcgcgct ggatattgta aaacgcgatt atgaaaagaa
     6841 attaacgcgc gatgatgagg cggaaaagtg agaaataaat ggggccgcta aatgtcccct
     6901 aagattgtgg gcaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacaccgctc
     6961 ctgcaatata ttgaattctt gcggtttcgt aggccggata aggcggtcac gccgcatccg
     7021 gcatgaacaa cgcgcacttt gttagtaatc tcggggcaac taaatgtcgc cccctttggt
     7081 tattacccaa ccgctggtgt cggatcgtcg cctttgtagt cgcgtttgac ttccgtcacc
     7141 tcatcgccct tctcgttgtg cagatgcact tcaagctggt taaaggcaat gttgatgtcg
     7201 ttttcacggc acaactgatc gatagtacgg ttcagctcat cgacagtacg gctacggtca
     7261 cgcagttcac gcacatacag acgcagctca tgatccaacg tgctggcacc aaatgccgta
     7321 aagaagactt ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc cgccttcagc
     7381 aacactttac gcactttttc cagatcggag ccataagcta cgccgagacg gatcaccaga
     7441 cgcgtagtcg tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa cgctttgttc
     7501 gggataatca cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat acggatcttg
     7561 ctgaccgtac ccgagaagct accaatggtt accgtgtcgc ctatgcgcac cggacgttcg
     7621 aacagaatga tcaaaccgga aacgaagtta ccgaagatct cttgtaaacc aaaaccaaga
     7681 cctacggata atgctgccgc cagccactgg agtttatccc acgagacgcc cagcgatccg
     7741 aacaccgtca tcgcaccaac ggcaatgatg atgtagttaa ggatagtggt gatggcatac
     7801 gatgccccct ggcgcatatt cagtcgcgag agcaccagca cttccagtaa accaggcagg
     7861 ttgcgaatca acgcccaggc caccattgag gcgataatcg caaacaacag actgcccatg
     7921 gtgacgtttt tcaccaccgc agcaccagct tcagtaccgt tgtaatgcca gagcgtaatg
     7981 ctgtcgagat agctgaacac ggtgatcaaa tcagaccaaa ttgcccagaa catgacaccg
     8041 aacagcgcaa acatcagcaa catggtaata cgcagcgtct gctggttaac ctgctccagc
     8101 gcaatggtgg gttcttccgg cggttcggca ccttctgcgc cctctttcac cagattctgc
     8161 cgacgcgcca gtgcacgacg ccaggcgata cgccgcgccg ctacgcttaa gccgcgcaat
     8221 accgtctggt acagcaggtt ccagatgatc accaaataaa cggtttcaat ccaacgcccg
     8281 gagaggcgca gcgtggtgta gaagtaaccc gtggcggtca gcaccatcaa cgcaatcggg
     8341 ataatcgaca gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt atcacgccag
     8401 ctttcgcggc acatcggcca caccaggaag gcaatcagca gcaggttgaa gaaaatcatc
     8461 gcctgcccca gcacatcatc catcagatgc agcggggaga gttctgccac cacagaccag
     8521 aaatggatcg gcagcaacgc gaggctgatg cggacaattt gccgacgcca gtggctggtc
     8581 tgctgttccg gcataccaaa gtgacgcacg gcaacaccat ttttctccag cactttccag
     8641 cacaggccaa ataccagcca gaaaatcgcc agttttttgc tgaacgacca caataactcg
     8701 ctgatattga gctgcatagt cagcagaatc aggccgacag cgagaataat caggcacacc
     8761 ggcagtgcgc ggatcaggtc gataaggatc gcttttggtg tgttgagctg gctgtcgtta
     8821 cgcagggaac ccacagcgga agccagtttt tgttgatacg ctttcagcca gcccagacgc
     8881 cagtggatca gcccggcaat caacagcagc ggcaaaccag cgaggaaggc gatgaatacg
     8941 gcgggccagg ctttttccca gttcaccgtg attttcatcg acttaaattc atctttcagc
     9001 gtttgcggga acgctttaat ccagtcccag tccatcgggc ggttactgtt cacccaaaaa
     9061 atttgctgcg tcaggatgga tttcaggttt ttcgacacac tcattaactg ctgctggttg
     9121 atttgcaggt taatggccat catcaactgg ttacccaact gtttgttaag ctgatccagc
     9181 aattcgcgac gcatatcaac tacttgcaat aatgcatcgt gaacttcgct gttgacttcg
     9241 ttggtgtgac cttcttccag tttgctgacg aacgcatcgc tctggaacag cgcgtcacgc
     9301 tgctggttga cttcaaactg ttcgagacgc aaatccgcga tgcggttggt catgttttcc
     9361 agttcatctg ccgaggggag tgtttgttgt tgctggtaca ggatacgtga caacagcaga
     9421 ctgcccttca gaacggcaat ctgctcctta atattacgtt ccgattgcag cgcacgctcc
     9481 agccagtttt tgactttaat gttttgctgc atcaactgat taccgttttg agtcgcggta
     9541 atcagacgct gacttaactg ctggttaatt tccagttcct gcttcaccag cggattagcc
     9601 tgaatacgcg cggcttcatc cggggagaca gcttcctgcg ccgttttttc ggttaaagtc
     9661 aggcgcttgc tgtttaccgc ttcttgcaac agttgtaact ggtgctccag acgggcgctg
     9721 ttcgccgtca cgtaatcacg ctgcttttgc aaggtatcct gcaagacagt gttcccttcc
     9781 aggcttttac gctgctggtc aatctcggca ttcagcaaag cctgctggac ctgcattaac
     9841 actttctggc tgggacgtaa ggcagtctcg ccgacatcag tcccatccag acgactgcga
     9901 atttgttgca gctgctgcga agcgttatac atcgcatttt gtacgcgttc gggctgcgtc
     9961 tgtagcgaaa ccagttggct gttataagac gccagatcgt tttgtgcgtt ttgcaaatcg
    10021 tccagcgcct gggcaacgcg agtttccagc tggcgcaacg aaagcgtgct cagaattttg
    10081 cgcgtttctt cgtcgttatc gacatcacta agtgctgtta acgccgcggt cgcctggcgc
    10141 attttttccg gcgcttcagc gactttttgc cgtagctgaa ctgtctcttc ttttacgcga
    10201 tcgattttat cgagggtggc taatgtatct gtcagatcct gctgcaccag tttgtcctga
    10261 gcagaaagat ctttttgttt attcagtgag tcaagttgtg cctgcaggtc cgcttttgtc
    10321 ggtaaatcac cattcgatga cgcccgtgca aacgccgtgt tctggcataa caagacaaaa
    10381 acaaaagcaa taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata ctggaacata
    10441 gtcatgatga atgaaggttt ctgaacctga agaacgacct gaaaaagtca aaccgcaaga
    10501 atatcacgac gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa attcctggag
    10561 tcagattcag ggttattcgt tagtggcagg gttacgaagc gtggggcaca ggagatacat
    10621 ctccagtaag atggcgacgt aatcgcgggc ttctttttta agatcaaaag attgcggggc
    10681 aaagagccag ttttccatca ggccggaaat atagccgcgc ataataattg ctgcgcgacg
    10741 cgtcattaaa tccgcaggca acattttcgc ttcaatacaa tgttttaacg tttgttctat
    10801 acggtcataa ctttccagac agagattacg ttgtgcctgt tgcacaacag ccatttcgcc
    10861 gacaaattcg catttgtgga atataatctc catcaataat cgacgccgtt cttctgtcac
    10921 cgtggactca agaacatgaa ttaatatctc tcttaatact gagagtggat cgccagggaa
    10981 ttttgcctga tactcaagct cgagttcacc aatattggat tctgacagtt cccagatctc
    11041 actgaacaaa tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg taacgccagc
    11101 tgcttttgca atctcaccca gcgaggtgga tgataccccc tgctgtgaga aaagacgtag
    11161 agccacatcg aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt ttcgtgccat
    11221 atgttcgtga atttacaggc gttagattta catacatttg tgaatgtatg taccatagca
    11281 cgacgataat ataaacgcag caatgggttt attaactttt gaccattgac caatttgaaa
    11341 tcggacactc gaggtttaca tatgaacaaa aacagagggt ttacgcctct ggcggtcgtt
    11401 ctgatgctct caggcagctt agccctaaca ggatgtgacg acaaacaggc ccaacaaggt
    11461 ggccagcaga tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct gcagatcaca
    11521 accgagcttc cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc tcaagttagc
    11581 gggattatcc tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagcagg tgtctctctc
    11641 tatcagattg atcctgcgac ctatcaggcg gcatacgaca gtgcgaaagg tgatctggcg
    11701 aaagcccagg ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca gaaattgctc
    11761 ggtactcagt acatcagtaa gcaagagtac gatcaggctc tggctgatgc gcaacaggcg
    11821 aatgctgcgg taactgcggc gaaagctgcc gttgaaactt cgcgaatcaa tctggcttac
    11881 accaaagtca cctctccgat tagtggtcgc attggtaagt caaacgtgac ggaaggcgca
    11941 ttggtacaga acggtcaggc gactgcgctg gcaaccgtgc agcaacttga tccgatctac
    12001 gttgatgtga cccagtccag caacgacttc ctgcgcctga aacaggaact ggcgaatggc
    12061 acgctgaaac aagagaacgg caaagccaaa gtgtcgctga tcaccagtga cggcattaag
    12121 ttcccgcagg acggtacgct ggaattctct gacgttaccg ttgatcagac cactgggtct
    12181 atcaccctac gcgctatctt cccgaacccg gatcacactc tgctgccggg tatgttcgtg
    12241 cgtgcacgtc tggaagaagg gcttaatcca aacgctattt tagtcccgca acagggcgta
    12301 acccgtacgc cgcgtggcga tgccaccgta ctggtggttg gcgcggatga caaagtggaa
    12361 acccgtccga tcgttgcaag ccaggctatc ggcgataagt ggctggtgac agaaggtctg
    12421 aaagcaggcg atcgcgtagt aataagtggg ctgcagaaag tgcgtcctgg tgtccaggta
    12481 aaagcacaag aagttaccgc tgataataac cagcaagccg caagcggtgc tcagcctgaa
    12541 cagtccaagt cttaacttaa acaggagccg ttaagacatg cctaatttct ttatcgatcg
    12601 cccgattttt gcgtgggtga tcgccattat catcatgttg gcaggggggc tagcgatcct
    12661 caaactgccg gtggcgcaat atcctacgat tgcaccgccg gcagtaacga tctccgcctc
    12721 ctaccctggc gctgatgcga aaacagtgca ggacacggtg acacaggtta tcgaacagaa
    12781 tatgaacggt atcgataacc tgatgtacat gtcctctaac agtgactcca cgggtaccgt
    12841 gcagatcacc ctgacctttg agtctggtac tgatgcggat atcgcgcagg ttcaggtgca
    12901 gaacaaactg cagctggcga tgccgttgct gccgcaagaa gtacagcagc aaggggtgag
    12961 cgttgagaaa tcatccagca gcttcctgat ggttgtcggc gttatcaaca ccgatggcac
    13021 catgacgcag gaggatatct ccgactacgt ggcggcgaat atgaaagatg ccatcagccg
    13081 tacgtcgggc gtgggtgacg ttcagttgtt cggttcacag tacgcgatgc gtatctggat
    13141 gaacccgaat gaactgaaca aattccagct aacgccggtt gatgtcatta ccgccatcaa
    13201 agcacagaac gcccaggttg cagctggtca gctcggtggt acgccgccgg tgaaaggcca
    13261 acagcttaac gcctctatta ttgctcagac gcgtctgacc tctactgaag agttcggcaa
    13321 aatcctgctg aaagtgaatc aggatggttc ccgcgtgcta ctgcgtgatg tggcgaaaat
    13381 tgagctgggt ggtgagaact acgacatcat cgcagagttt aacggccaac cggcttccgg
    13441 tctggggatc aagctggcga ccggtgcgaa cgcgctggat accgctgcgg caatccgtgc
    13501 tgaactggcg aagatggaac cgttcttccc gtcgggtctg aaaattgttt acccgtatga
    13561 caccacaccg ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg tcgaagcgat
    13621 catcctcgtg ttcctggtaa tgtatctgtt cttgcagaac ttccgcgcga cgttgattcc
    13681 gaccattgcc gtaccggtgg tattgctcgg gacctttgcc gtccttgccg cctttggctt
    13741 ctcgataaac acgctaacaa tgttcgggat ggtgctcgcc atcggcctgt tggtggatga
    13801 cgccatcgtt gtggtagaaa acgttgagcg tgtgatggcg gaagaaggtt tgccgccaaa
    13861 agaagccacc cgtaagtcga tggggcagat tcagggcgct ctggtcggta tcgcgatggt
    13921 actgtcggcg gtattcgtac cgatggcctt ctttggcggt tctactggtg ctatctatcg
    13981 tcagttctct attaccattg tttcagcaat ggcgctgtcg gtactggtgg cgttgatcct
    14041 gactccggct ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc acggggaagg
    14101 taaaaaaggc ttcttcggct ggtttaaccg catgttcgag aagagcacgc accactacac
    14161 cgacagcgta ggcggtattc tgcgcagtac ggggcgttac ctggtgctgt atctgatcat
    14221 cgtggtcggt atggcctatc tgttcgtgcg tctgccaagc tccttcttgc cagatgagga
    14281 ccagggcgta tttatgacca tggttcagct gccagcaggt gcaacgcagg aacgtacgca
    14341 gaaagtgctc aatgaggtaa cgaattacta tctgaccaaa gaaaagaaca acgttgagtc
    14401 ggtgttcgcc gttaacggct tcggctttgc gggacgtggt cagaataccg gtattgcgtt
    14461 cgtttccttg aaggactggg ccgatcgtcc gggcgaagaa aacaaagttg aagcgattac
    14521 catgcgtgca acacgtgctt tctcgcaaat caaagatgcg atggttttcg cctttaacct
    14581 gcccgcaatc gtggaactgg gtaccgcaac cggctttgac tttgagctga ttgaccaggc
    14641 tggccttggt cacgaaaaac tgactcaggc gcgtaaccag ttgcttgcag aagcagcgaa
    14701 gcaccctgat atgttgacca gcgtacgtcc aaacggtctg gaagataccc cgcagtttaa
    14761 gattgatatc gaccaggaaa aagcgcaggc gctgggtgtt tctatcaacg acattaacac
    14821 cactctgggc gctgcatggg gcggtagcta tgtgaatgac tttatcgacc gcggtcgtgt
    14881 gaagaaagtt tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg atatcggcga
    14941 ctggtatgtt cgtgctgctg atggtcagat ggtgccgttc tcggcgttct cctcttctcg
    15001 ttgggagtac ggttcgccgc gtctggaacg ttacaacggc ctgccatcta tggaaatctt
    15061 aggccaggcg gcaccgggta aaagtaccgg tgaagcaatg gagctgatgg aacaactggc
    15121 gagcaaactg cctaccggtg ttggctatga ctggacggga atgtcctatc aggaacgtct
    15181 ctccggcaac caggcacctt cactgtacgc gatttcgttg attgtcgtgt tcctgtgtct
    15241 ggctgcgctg tacgagagct ggtcgattcc gttctccgtt atgctggtcg ttccgctggg
    15301 ggttatcggt gcgttgctgg ctgccacctt ccgtggcctg accaatgacg tttacttcca
    15361 ggtaggcctg ctcacaacca ttggtttgtc ggcgaagaac gcgatactta tcgtcgaatt
    15421 cgccaaagac ttgatggata aagaaggtaa aggtctgatt gaagcgacgc ttgatgcggt
    15481 gcggatgcgt ttacgcccaa tcctgatgac ctcgttggcg tttatcctcg gcgttatgcc
    15541 gctggttatc agtactggtg ctggttccgg cgcgcagaac gcagtaggta ccggtgtaat
    15601 gggcgggatg gtgaccgcaa cggtactggc aatcttcttc gttccggtat tctttgtggt
    15661 ggttcgccgc cgctttagcc gcaagaatga agatatcgag cacagccata ctgtcgatca
    15721 tcattgatac aacgtgtaat cactaaggcc gcgcaagcgg ccttttttat gcataaccta
    15781 cgaacattaa ggatgtattg aaccaccaac tcaggatctc atacgaaaac cagtattaac
    15841 cacgtataaa attcataaaa
//
LOCUS       DAZFRE010000103        15812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFRE010000103 DAZFRE010000000
VERSION     DAZFRE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.103"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..331)
                     /locus_tag="ACHKUI_004000"
     CDS             complement(<1..331)
                     /locus_tag="ACHKUI_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6600093.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
     gene            483..812
                     /locus_tag="ACHKUI_004001"
     CDS             483..812
                     /locus_tag="ACHKUI_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6600094.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(869..1612)
                     /locus_tag="ACHKUI_004002"
                     /pseudo
     CDS             complement(869..1612)
                     /locus_tag="ACHKUI_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            1702..2133
                     /locus_tag="ACHKUI_004003"
     CDS             1702..2133
                     /locus_tag="ACHKUI_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6600095.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            2342..2587
                     /gene="nrdH"
                     /locus_tag="ACHKUI_004004"
     CDS             2342..2587
                     /gene="nrdH"
                     /locus_tag="ACHKUI_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6600096.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2584..2994
                     /gene="nrdI"
                     /locus_tag="ACHKUI_004005"
     CDS             2584..2994
                     /gene="nrdI"
                     /locus_tag="ACHKUI_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6600097.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2967..5111
                     /gene="nrdE"
                     /locus_tag="ACHKUI_004006"
     CDS             2967..5111
                     /gene="nrdE"
                     /locus_tag="ACHKUI_004006"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6600098.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTCYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYSKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            5121..6080
                     /gene="nrdF"
                     /locus_tag="ACHKUI_004007"
     CDS             5121..6080
                     /gene="nrdF"
                     /locus_tag="ACHKUI_004007"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6600099.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6437..7639
                     /gene="proV"
                     /locus_tag="ACHKUI_004008"
     CDS             6437..7639
                     /gene="proV"
                     /locus_tag="ACHKUI_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6600100.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7632..8696
                     /gene="proW"
                     /locus_tag="ACHKUI_004009"
     CDS             7632..8696
                     /gene="proW"
                     /locus_tag="ACHKUI_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6600101.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8753..9745
                     /gene="proX"
                     /locus_tag="ACHKUI_004010"
     CDS             8753..9745
                     /gene="proX"
                     /locus_tag="ACHKUI_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6600102.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9937..11121
                     /locus_tag="ACHKUI_004011"
     CDS             9937..11121
                     /locus_tag="ACHKUI_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600103.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11245..11982
                     /gene="ygaZ"
                     /locus_tag="ACHKUI_004012"
     CDS             11245..11982
                     /gene="ygaZ"
                     /locus_tag="ACHKUI_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6600104.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGFLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11972..12307
                     /gene="ygaH"
                     /locus_tag="ACHKUI_004013"
     CDS             11972..12307
                     /gene="ygaH"
                     /locus_tag="ACHKUI_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6600105.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12398..12928
                     /gene="emrR"
                     /locus_tag="ACHKUI_004014"
     CDS             12398..12928
                     /gene="emrR"
                     /locus_tag="ACHKUI_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6600106.1"
                     /translation="MDSSFTPIEQMLKFLASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            13055..14227
                     /gene="emrA"
                     /locus_tag="ACHKUI_004015"
     CDS             13055..14227
                     /gene="emrA"
                     /locus_tag="ACHKUI_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6600107.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14244..15782
                     /gene="emrB"
                     /locus_tag="ACHKUI_004016"
     CDS             14244..15782
                     /gene="emrB"
                     /locus_tag="ACHKUI_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6600108.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRIGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
BASE COUNT         3741 a         4086 c         4077 g         3908 t
ORIGIN      
        1 caaataaacc attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac
       61 cgtaatgctc atgacgttca cccgctaaat agagcggaaa ttgctggtaa tgatcgcaag
      121 ttttaatatc ggaagcgggt tcagccagtg ttgaactacg ttcttttaat gttggatgaa
      181 tcaccagggc ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac
      241 ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta
      301 cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttattat caaccgcggg
      361 cctaccttac ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga
      421 acctcagtta agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc
      481 atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg
      541 atgtcaatca attagctgac agtctcgaat ctgtattgaa gtcctggggc agcgacgcca
      601 aaggggaagc cgaagccgct cgtagtaaag cgcaggctct actgaaagaa accagggcgc
      661 gaatgcatgg tcgtactcgc gtccagcaag ccgcgcgcga tgccgttggc tgcgcggatt
      721 cttttgttcg tgaaagaccc tggtgtagtg tgggtacagc ggcagcggtg ggtattttta
      781 tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt
      841 tgaccccgaa gcctggcttc ggggtttttt agcttaaggc tttacgtaat tgttgtgcta
      901 cctgtcgcgc catagcttcg ttaacgatat tagtaaacga aagcagtaat ccaccttccc
      961 cacttgaacg gatacgccag tcgctaagtg cctgcaccgc aaggcctgca gcattggctt
     1021 tacgcgcatg ggcaatatca tcgcctatca ttctgatcac catctggata ccacctttct
     1081 gcggcacaac ctgaaatcct tgctgcgtca acgcttgctc aatccattgc cgacgttgag
     1141 cataatgctg acgcattttc ttcagatgcc gccagaaatg cccctcgcga agaaagtctg
     1201 ccagtgtgtt ctgccataga acaggtaccg cacatggagc cagtgacgcc tggtggcgga
     1261 attgtgtaat ttgcttcacc ggcaccacca gccacgcaca gcgcaatgca ggaaatagcg
     1321 ctttgctgaa tgttccggca taaattaccc gctgcggtgc gtcgagactt tttaacgccg
     1381 gtaacggctt accgtgatag cgaaactcac tgtcataatc atcttcaata atccatgcct
     1441 gactacgatc tgcccattcc agtatctgat gcctgcgcgc taaagagagc gccacaccca
     1501 gcggactttg gtgtgctggt gttatcaggg caaaacgcgc atcaggataa ttttgtattc
     1561 cgcttgtgat atccagtcca ttgtcatcaa ccggcacagg caaaatttcc acatctggtc
     1621 tgctttttta ggtaatttat ggctctcttt aacagtccat tatagtgcta cattgcgggc
     1681 gttaataaga ggagaaactg tatgactaca ttacgtcagc cttactacga acttagcccg
     1741 gcagtgtata acgcactagt gcaggccaaa acggcactgg aaaatagcac gctggatacc
     1801 acgttgatgg agttagttta tttgcgcgtc tcgcaaatca acggctgcgc attttgtctg
     1861 gagatgcaca gcaaagcatt gcgcaaatcc ggcgtgccac agcacaaact ggacgccctg
     1921 gcaggttggc gcgtaagcca tcattttgat gaacgcgagc gggcggcgct ggcatgggca
     1981 gaatcggtaa ccgaaattgc cagaacccat gcggaagacg aggtttatca gcctttgctt
     2041 gagcatttca gcgcagcaga gatcagcgac ttaacgtttg ccatcgggct gatgaattgt
     2101 tttaaccgtc tggccgtttc catgcggatg taacttcttg atgccgggta tttgcccggc
     2161 aaaaaaacac aaaatcccct accccgttga gccattcatc ggtggcgtaa tcaatctaaa
     2221 attcccatat cttgtgtggt tgaatctttt ttcaactaca tctagtatct ctgtatcaac
     2281 agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg aaatacgaat
     2341 catgcgcatt actatttaca ctcgtaacga ttgcgtccag tgccacgcca ccaaacgggc
     2401 gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc ctgaagcggc
     2461 agaagcgctg cgtgctcagg gctttcgtca gttgccggtt gtgattgcag gcgatcttag
     2521 ctggtctggt ttccgcccgg acatgatcaa tcgtctgcat ccagcgccac acgcggccag
     2581 tgcatgagcc agctcgtcta cttttccagc agctccgaaa atacgcagcg ttttatcgaa
     2641 cgtttagggc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat tcaggtagac
     2701 gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg cgcggtgcca
     2761 cgacaggtca ttcgcttttt aaacgacgag cataaccggg cgttgctgcg cggcgttatt
     2821 gcttcgggta atcgcaactt tggtgaggca tatggccgcg ccggagatgt gattgcccgg
     2881 aaatgcggcg tgccgtggct gtaccgcttt gaactcatgg gtacgcaaag cgatatcgaa
     2941 aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaacgc ctgacgcagg
     3001 aaacgatgga ttatcacgct ctgaatgcga tgcttaacct ttacgatagc gcaggtcgca
     3061 ttcagttcga taaagacagc caggcagtag acgcctttat gacgacgcat gtgcgtccga
     3121 acagtgtggc cttcagtagc cagcagcagc gcctgaactg gctggttaac gaaggttact
     3181 atgatgaaag tgtccttaat cgctactctc gcgattttgt cattacgctg tttgcccacg
     3241 cgcacgccag cggttttcgt ttccagacat tcctcggagc atggaagttt tacacctgct
     3301 atacgttgaa gacattcgac ggtaagcgtt atctggaaga ttttgccgat cgcgtcacga
     3361 tggtggcgct gacgctggca caaggcgatg aaacactggc gacgcagctc actgatgaga
     3421 tgctgtcagg acgctttcag ccagccacgc caacattcct caactgcggt aagcagcagc
     3481 gcggcgaact ggtttcctgt tttctgctgc gtattgaaga caatatggag tcgattggcc
     3541 gggcagtaaa ttccgcacta caactgtcga aacgcggcgg cggcgtagca tttttgctgt
     3601 cgaatctgcg ggaagcgggc gcgccaatta aacgtattga aaatcaatct tctggcgtga
     3661 ttccggtgat gaaaatgctg gaagacgcat tttcctatgc caaccaactc ggcgcgcgtc
     3721 agggggctgg ggcagtctat ttacatgctc atcatcccga tattctgcgt tttctcgata
     3781 caaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctg ggcgtggtga
     3841 tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg ttttcgccct
     3901 atgacgtaga gcgagtttat agcaagccgt ttgccgatgt cgccatcagt gaacactatg
     3961 acgaactggt tgccgatgaa cgcatccgca aaaaatacct caacgcccgt gatttcttcc
     4021 agcgactggc agaaatccag tttgagtccg gctatccgta catcatgtat gaagacacgg
     4081 ttaaccgtgc taaccctatc gccgggcgca taaatatgag caatctctgc tcagaaattt
     4141 tgcaggttaa cagcgcctca gaatatgacg agaatctcga ctatgctcgt acaggacatg
     4201 atatttcctg caatctaggg tcgttgaata ttgcccacac catggattcc cccgactttg
     4261 cccgcacggt agagactgcc gtacgcggtt taacggcggt gtcagatatg agtcatatcc
     4321 gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcaca cgccatcggt ctggggcaga
     4381 tgaatttaca cggttatctg gcacgagaag gcatcgctta tgggtcgccg gaagcactgg
     4441 atttcaccaa tctctatttc tataccatca cctggcacgc actgcgtacc tcgatgttgc
     4501 tggcgcgcga acgcggtgaa accttcgccg ggttcaaaca gtcacgctat gccagtggtg
     4561 aatatttcag ccaatatctg caaggtaact ggcagccgaa aacggcgaaa gttggcgaac
     4621 tgtttgcccg tagcggtatt acgttaccta cccgtgagat gtgggcacag ctgcgcgacg
     4681 acgtgatgcg ctatggtatc tacaaccaga atctccaggc ggtgccgcca accggttcta
     4741 tctcttatat caaccatgcc acgtcgagta ttcatccgat tgtggcgaaa gtagagatac
     4801 gcaaagaggg caaaacagga cgcgtttact accctgcccc gtttatgact aacgaaaatc
     4861 tggcgctgta tcaggacgct tacgaaatcg gcgcagaaaa gatcatcgac acctacgcgg
     4921 aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc gataccgcca
     4981 ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caagggtatc aaaacgctct
     5041 attacattcg cctgcgtcag atggcgctgg aaggcactga aattgaaggc tgcgtctcct
     5101 gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac tggaacaaga
     5161 tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc tggctaccag
     5221 aaaaggtgcc actgtcgaac gatattcctg catggcagac attaagtgtc gtagaacaac
     5281 aactaacaat gcgcgttttt actggcctga cgctgctcga cacgctgcaa aatgttatcg
     5341 gcgcgccctc gctgatgccc gatgcactca ccccccatga agaggcggta ttatcaaata
     5401 tcagctttat ggaagcggtt catgcccgct cttacagttc aattttctcg acgctgtgcc
     5461 agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcaccg ttgcagcgaa
     5521 aagctcagat tattcagcaa cattatcgcg gggatgatcc gctgaaaaag aaaatcgcca
     5581 gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg tatttttcca
     5641 gccgcggaaa gctaaccaat accgcagacc tgatccgtct gattatccgg gatgaagcag
     5701 tccacggtta ttacatcggc tataaatatc agaaaaacat ggaaaagatc tcccaggcac
     5761 aacgtgaaga gttgaagagt ttcgccttcg atttgttgct ggaactttat gacaacgagt
     5821 tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg aaagcgtttc
     5881 tctgttacaa cgccaataag gcgttgatga atctgggcta cgaaccgtta tttcccgcag
     5941 aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc gccgaatgcc gatgaaaatc
     6001 acgatttctt ttccggttca ggctcttcat atgtgatggg aaaagcggtc gaaacagaag
     6061 atgaagactg gaatttctga gggtgttatt tttcaaaaat atcactaccc gcagcatgga
     6121 aataattccc gccaaatagc tttttatcac gcaaatattt tgtggtgatc tacactgaca
     6181 ctctgttgca ttattcgcct gaaaccacaa tattcaggcg ttttttcgct atctttgaca
     6241 aaaaatatca acttttctcg atttgctctc agcccttata tcatgggaaa ttccggcgat
     6301 ttgctcacat caatattcat gccacatttg ccatcagggg ttgcctcaga ttctcagtat
     6361 gttagggtag aaaaaagtga ctatttccat tgggtaatgt atcgacataa acaaataaca
     6421 ggaatctttc tattgcatgg caattaaatt agaaattaaa aatctttata aaatatttgg
     6481 tgagcatcca cagcgagctt tcaaatatat cgaacaagga ctttcaaaag aacaaattct
     6541 ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt ctggccattg aagaaggcga
     6601 gatatttgtc atcatgggat tatccggttc gggtaaatcc acaatggtac gccttctcaa
     6661 tcgcctgatt gaacccaccc gcgggcaagt gctgattgat ggtgtggata ttgccaaaat
     6721 atccgacgcc gaactccgtg aggtgcgcag aaaaaagatt gcgatggtct tccagtcctt
     6781 tgccttaatg ccgcatatga ccgtgctgga caatactgca ttcggtatgg aattggccgg
     6841 aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca ctgcgtcagg tcgggctgga
     6901 aaattatgcc cacagctacc cggatgaact ctctggcggg atgcgtcaac gtgtgggatt
     6961 agcccgcgcg ttagcgatta atccggatat attattaatg gacgaagcct tctcggcgct
     7021 cgatccatta attcgcaccg agatgcagga tgagctggta aaattacagg cgaaacatca
     7081 gcgcaccatt gtctttattt cccacgatct cgatgaagct atgcgtattg gtgaccgaat
     7141 tgccattatg caaaatggtg aagtggtgca ggttggtaca ccggatgaaa ttcttaataa
     7201 tccggcgaat gattatgtcc gaaccttctt ccgtggcgtc gatattagcc aggtgttcag
     7261 tgcgaaagat attgcccgtc gtacaccgaa cggcttaatt cgtaaaaccc ccggcttcgg
     7321 cccgcgttcg gcactgaaat tattgcagga tgaagatcgc gaatatggct acgtcatcga
     7381 acgcggtaat aagtttgtcg gcgctgtgtc tatcgattcg cttaaagccg cgttaacgca
     7441 gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta gcggtcgatg cacaaacgcc
     7501 tcttagcgaa ttgctctctc atgtcggaca ggcaccctgt gcggtgcccg tggtcgacga
     7561 ggaccaacag tatgtcggca tcatttcgaa aggaatgctg ctgcgcgctt tagatcgtga
     7621 gggggtaaat aatggctgat caaaataatc cgtgggatac cacgccagcg gcggacagtg
     7681 ctgcacaatc cgcagacgcc tggggtacac cggcgactgc accgactgac ggcggtggcg
     7741 ctgactggct gaccagtacg cctgcgccaa acgtcgagca ttttaatatt ctcgatccgt
     7801 tccataaaac gctgatcccg ctcgacagtt gggtcactga agggatcgac tgggtcgtta
     7861 cccattttcg tcccgtcttt cagggcgtgc gcgttccggt tgattacatc ctcaacggtt
     7921 tccagcaatt gctgctgggt atgcccgcgc cggtggcgat tatcgttttc gctctcatcg
     7981 cctggcagat ttccggggtc ggaatgggcg tggcgacgct ggtttcgcta attgccatcg
     8041 gcgcaatcgg tgcctggtca caggcaatgg ttaccctggc gctggtgtta acagccctgc
     8101 tgttctgtat cgtcataggt ttgccgttgg ggatctggct ggcgagaagt ccgcgagcgg
     8161 cgaaaattat tcgtccactg cttgatgcca tgcagaccac gcccgcgttt gtttatctgg
     8221 tgccaatcgt catgctgttt ggtatcggca acgtgccagg cgtggtggtg acgatcatct
     8281 ttgcgctgcc gccgattatc cgtctgacga ttctgggaat taaccaggtt ccggcggatc
     8341 tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca gatgctgttc aaagttcagt
     8401 taccgctggc gatgccaacc attatggcgg gcgttaacca gacgctgatg ctggcccttt
     8461 ctatggtggt catcgcctcg atgattgccg tcggcgggct gggtcagatg gtacttcgcg
     8521 gtatcggtcg tctggatatg gggcttgcca ccgttggcgg cgtcgggatt gtgatcctcg
     8581 ccattatcct cgaccgcctg acgcaggccg ttgggcgcga ctcacgcagt cgcggcaatc
     8641 gtcgctggta caccactggc cctgtcggtc tgctgacccg cccattcatt aagtaactct
     8701 gcacttgccc ggtgacgccg ggcatatcac cctgccaaaa aaaggaataa caatgcgaca
     8761 tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt ttgctgccga
     8821 cctgccgggc aaaggcatta ctgttaatcc agttcaaagc accatcaccg aagaaacctt
     8881 ccagacactg ctggtaagtc gtgcgctgga gaaattaggt tataccgtca ataaacccag
     8941 cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa ccttcaccgc
     9001 agtgaactgg acgccactgc atgacaacat gtacgaagct gccggtggcg ataagaaatt
     9061 ttatcgtgaa ggagtatttg ttaacggcgc ggcacagggt tacctgatcg ataagaaaac
     9121 tgccgaccag tataaaatta ccaacatcgc gcaacttaaa gatccgaaga tcgccaaact
     9181 gttcgatacc aacggcgacg gtaaagcgga tttaaccggc tgtaacccag gctggggctg
     9241 cgaaggtgcg atcaaccacc agcttgccgc gtatggactg accaataccg tgacgcataa
     9301 tcaggggaac tacgcagcga tgatggctga caccatcagt cgttacaaag agggtaagcc
     9361 ggtgttttac tacacctgga cgccgtactg ggtgagtaac gaactgaaac cggggaaaga
     9421 tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg ccgataccaa
     9481 actgccgaat ggcgcgaatt acggcttccc ggtcagtacc atgcatatcg ttgccaacaa
     9541 agcctgggcc gagaaaaacc cggcagcagc gaaactgttt gccattatgc agttaccagt
     9601 ggctgatatt aacgcccaga acgccatcat gcatgacggc aaagcctcag aagacgatat
     9661 tcagggccac gttgatggtt ggatcaaagc ccaccagcag cagttcgatg gctgggtgaa
     9721 tgaagcgctg gcagcgcaga agtaattttt attcgggcgg ataaggcgtt tttgcctcat
     9781 ccgccgctct gtacacaatg cctgatgcga cgctggcgcg tcttatcata cctacaaacc
     9841 acaccgcaca tttcaacaat ctatctttca tcccatattc atcaacatcc gctattattg
     9901 atttccagct taatcatcac ctgatgaaca aaaataatga ctaaacctaa tcatgagctt
     9961 agcccggcgc tgatcgtgct gatgtctatc gccaccggtc tggcggtagc cagtaactat
    10021 tacgcccagc cattgctcga caccatcgcg cgtaactttt ccctttccgc cagttcggca
    10081 ggctttattg ttaccgccgc acagttgggc tatgccgcag gtctgctgtt tcttgttccc
    10141 ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact ggcagcaggc
    10201 ggcatgttaa tcactgccag cagtcagtcg ctggcgatga tgatcctcgg tacagcatta
    10261 accggtttat tttcagtcgt ggcacaaatt ctggttccac tggcagcgac actggcttca
    10321 ccggacaaac gcggcaaagt ggttggcacc attatgagcg gtctgctatt ggggatctta
    10381 ctcgcacgga ccgttgccgg attgctggcg aatctcggcg gctggcgcac cgtcttttgg
    10441 gttgcttcga tgttaatggc actgatggcg ctggcattat ggcgtggtct gccacaaatg
    10501 aaatcagaaa cccacctcaa ctacccacag ttgttgggtt ccgttttcag catgtttatc
    10561 agcaataaga tcctgcgcac ccgcgcgttg ctgggctgcc tgacctttgc caacttcagc
    10621 attctctgga cctcaatggc ctttttgctt gccgctccac cttttaacta cagcgatggt
    10681 gtaattggtc tgtttgggct tgcgggagct gccggagcgt tgggcgctcg tccggcgggc
    10741 ggttttgccg ataagggcaa atcgcaccac accacaactt tcggtctgct gctgctgtta
    10801 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgctgat tatcggcatc
    10861 ctggtgctgg acctcaccgt gcagggcgtg catatcacta accagacggt aatttatcga
    10921 atacaccctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    10981 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtg
    11041 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    11101 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    11161 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    11221 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    11281 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    11341 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    11401 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    11461 gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa tggatgttcg ccatgttttg
    11521 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgccctg
    11581 tgggcgtttg gcctgacgga tgaggttttt gccgccgcaa ccgcaaaact ggtacgcaat
    11641 aatcgccgct ggagcgaaaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    11701 gtatttggta cggtaatagg ggcattctcc ggcagcggct ttctgcaagg ttatcccgcc
    11761 gttgaagcag cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    11821 ttccagcgca aacaatctct ttgcgttacc gcagcgttag ttggtgccct tgcaggcgta
    11881 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    11941 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    12001 actggttggc gcggcgaatt attgcttccg ctatctgccg ctgcgcctgc gtgtgggtaa
    12061 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    12121 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    12181 cgtgcccaca ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    12241 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    12301 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    12361 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    12421 tgaacaaatg ctaaaatttc tcgccagccg ccacgaagat tttccttatc aggagatcct
    12481 tctgacccgt ctttgcatgc atatgcaaag caagctgctg gaaaaccgca ataaaatgct
    12541 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    12601 aaaccacagt attcagcctt ctgaattgag ttgtgctctt ggatcatccc gtaccaacgc
    12661 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgtg aaagcgataa
    12721 cgatcgccgc tgtctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    12781 tttaccgccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    12841 agaccagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacagga
    12901 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    12961 aaagaaaaaa taaccggcca gcatcgtaac atgctggcct ttttggcaag caggtcggct
    13021 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    13081 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    13141 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    13201 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    13261 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgacgtgct
    13321 ggtcactctc gacccgacag atgctcgcca ggcatttgaa aaagccaaaa ctgcactggc
    13381 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    13441 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    13501 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    13561 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    13621 ggggacgaaa ctggaagatc agcctgccgt acaacaggct gccaccgaag tacgtaacgc
    13681 ctggctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tctcccgccg
    13741 cgcggtacag cctggggcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    13801 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgtatcgg
    13861 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    13921 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaacgccac
    13981 cggtaactgg atcaaagtcg ttcagcgtct gcctgtgcgt attgaactgg accagaaaca
    14041 gctggagcaa tacccgctgc gtatcggttt atccacgctg gtgagtgtca ataccactaa
    14101 ccgtgacggt caggtactgg caaataaagt acgttccaca ccggtcgcgg taagcaccgc
    14161 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    14221 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcacaactgg
    14281 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctggac tccaccattg
    14341 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc cagggaacgt
    14401 gggtgatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    14461 caaagcgcat cggggaagtg aaattgttcc tctggtcaac catcgccttt gctattgcgt
    14521 cgtgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    14581 ggattgtcgc cgggccgctg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    14641 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtt gcgccaattt
    14701 gcggcccgat cctcggcggt tatatcagtg ataattacca ctggggctgg atattcttca
    14761 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaactctg cgcggacgtg
    14821 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    14881 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactggttc tcatcacagg
    14941 aaattatcat cctcaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    15001 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgtaac ttcaccatcg
    15061 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgcgattgtt ctgctgccgc
    15121 agttgttgca ggaggtctac ggttacacgg cgacgtgggc agggctggcc tctgcgccgg
    15181 tagggattat tccggtgatc ctgtcgccga ttatcggccg ctttgcgcat aaactggata
    15241 tgcgccgact ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    15301 atacctttga accgggtatg gattttggcg cgtcggcctg gccacagttt atccagggtt
    15361 ttgcggtggc ctgcttcttt atgccgctga ccaccattac gctgtccggt ttgccaccgg
    15421 aacgactggc ggcggcatca agcctctcca actttacgcg aacgctggcg gggtctatcg
    15481 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    15541 ctgagtcggt aaacccgttc aacccgaatg ctcaggcgat gtacagtcaa ctggaagggc
    15601 ttggtatgac gcaacagcag gcatcaggct ggattgccca gcagataacc aatcaggggc
    15661 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtcctgctgg
    15721 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggt ggtgcgcact
    15781 aagtacaact aagccagttc atttgaactg gc
//
LOCUS       DAZFRE010000104        15670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFRE010000104 DAZFRE010000000
VERSION     DAZFRE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.104"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..551
                     /locus_tag="ACHKUI_004017"
     CDS             <1..551
                     /locus_tag="ACHKUI_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6600109.1"
                     /translation="EHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGVGKSGIIARK
                     LAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGN
                     YLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELK
                     KFTRADFGLYHPGGALGIKANS"
     gene            623..1570
                     /locus_tag="ACHKUI_004018"
     CDS             623..1570
                     /locus_tag="ACHKUI_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6600110.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1870..3246)
                     /locus_tag="ACHKUI_004019"
     CDS             complement(1870..3246)
                     /locus_tag="ACHKUI_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO6600111.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMTGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3246..3674)
                     /gene="tssE"
                     /locus_tag="ACHKUI_004020"
     CDS             complement(3246..3674)
                     /gene="tssE"
                     /locus_tag="ACHKUI_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6600112.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3678..4214)
                     /gene="tssJ"
                     /locus_tag="ACHKUI_004021"
     CDS             complement(3678..4214)
                     /gene="tssJ"
                     /locus_tag="ACHKUI_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6600113.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4195..5274)
                     /gene="tssG"
                     /locus_tag="ACHKUI_004022"
     CDS             complement(4195..5274)
                     /gene="tssG"
                     /locus_tag="ACHKUI_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6600114.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPDGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5238..6998)
                     /gene="tssF"
                     /locus_tag="ACHKUI_004023"
     CDS             complement(5238..6998)
                     /gene="tssF"
                     /locus_tag="ACHKUI_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6600115.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGIQQFWLRLPGQERRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPERITGFEIDV
                     VLAENWPHDLPFNTDNLRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLNLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            complement(7646..7978)
                     /locus_tag="ACHKUI_004024"
     CDS             complement(7646..7978)
                     /locus_tag="ACHKUI_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600116.1"
                     /translation="MTRVVIALIFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTANKDSSGNFDYPFVILSSGR
                     EKNLGKTI"
     gene            complement(7971..9389)
                     /locus_tag="ACHKUI_004025"
     CDS             complement(7971..9389)
                     /locus_tag="ACHKUI_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO6600117.1"
                     /translation="MGRGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            complement(9395..11005)
                     /gene="tssA"
                     /locus_tag="ACHKUI_004026"
     CDS             complement(9395..11005)
                     /gene="tssA"
                     /locus_tag="ACHKUI_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6600118.1"
                     /translation="MSELLQKLTRSCFANRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKE"
     gene            complement(10998..14326)
                     /locus_tag="ACHKUI_004027"
                     /pseudo
     CDS             complement(10998..14326)
                     /locus_tag="ACHKUI_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
     gene            complement(14383..15597)
                     /locus_tag="ACHKUI_004028"
     CDS             complement(14383..15597)
                     /locus_tag="ACHKUI_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600119.1"
                     /translation="MPVRLNTIPDAEPYPAPPAHIKWLSALLLMLSVGVLLTSLFASD
                     ELAKNGPHFWGLACGLPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILHETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFVQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
BASE COUNT         3571 a         4276 c         4050 g         3773 t
ORIGIN      
        1 acgaacataa agcagttgaa gaagttattg attcccctct ttcggaggtc gcaaatttaa
       61 ttaaagtatt acaatcttgt cagggaaaag ttgtttttat tggtgttggt aagtccggta
      121 ttattgccag gaaactcgcc gcaacatttg ccagcaccgg aactccctcg ttttttgttc
      181 acggtacgga agctgtacac ggcgaccttg gaatggtggc gaaagacgat gttgttattc
      241 ttatttcaaa cagtggtgag acggcggaaa tcctggcgac actgcctagt ctgaaaaaaa
      301 tgggtaatta tttgatctct tttacccgca gtcatcactc atcgcttgcg ataagttgtg
      361 atttatctgt tgaaatcccc gtcaaaagtg aggcggataa tttaggttta gcaccgtcat
      421 gctcgtcgac tgttgttctg gttgttggtg atgcggtggc gcttgcactc tctgaattga
      481 aaaaatttac tcgtgctgat ttcggcttat atcatccagg aggtgcactc ggcattaaag
      541 caaattcata atttcaaaat ataaatacag taaccggtca cgccatcttt tggggtggcg
      601 ttctttgaag tgagtgtccg ctatgaaaaa tgttctggtc accgtcccca gcttttcagc
      661 acgctgtgtt tccgccagca agttgctaag agagaataat tttaatctta ttattaagaa
      721 taatgtggag catctgctta aatcagaatc cactgcatta cgagaatcta tttgcgcggt
      781 catcgccgga aaagatggtt atcaggcgga cacgctatca ttgctgcctg gtgtgagaat
      841 aatatcgaga ttcgggacgg ggatagacaa tattgatctt cgtgcggcac agcaatccgg
      901 tattgttgtc aataatgcgg ttgggattaa ttcaaatgcg gttgcggaat ttatcattgg
      961 acttatcttc gcgagcatga gaaatatccc tggcagctat catgcgatgc aaaatggcta
     1021 ctggggtgag tcgcatggct gtgaattaca agggaaacgg attggcctgg ttggctacgg
     1081 taatataggt aaaactctgg cgaaaaggtt atccggtttt gatgttgagc ttttagcttt
     1141 tgacaaacaa cccgactatc aggttgctga caaagctgga gttcagtttg tatcaattga
     1201 agatatcttt atgcagtcgc atgtcatcat tgttcttttg cctttttctt ctgagctgga
     1261 gaacttcatt agtcataaat atttgtcgat gatgcgcaat ggtgctctga tcatcaatgc
     1321 cgcaagggga aagttgctgg atgagggcgc tttacttcag gtgattgaag aacgaaatgt
     1381 gtttgcggcg ctggatgtgt ttagctctga accgttggcg caatttagcc cactgctaca
     1441 tgccaaaaat atcattacaa cgccgcatat cgcggccgcc accgttgagt cataccagca
     1501 aaccgggata cacgttgccc agtcgataat tgattacttc gcaggaaggg agataaaaaa
     1561 cgtgttgtga gttcagcgaa caaagatttt aaatacaaaa aaaccgcaat ttgtagaaca
     1621 cattgcggtt tgtcagcatt ctaataaatt ggttgcgggg gccacagtca agaggttaat
     1681 ccaatctttt gaaacttaag gtattcactg ttcccgaacc caagctatct accgaaatat
     1741 acactcatta tctgatgtga atcaacgagt agatcgaatg gcctgttcct ctcttttttt
     1801 gtacacagag actcctttaa aaaatggcaa ggtcacatat cccttattgg tacataaatc
     1861 ccccgatgtt tactgacttt gttccacctt cgtatccagt aaaatgacat agcgattcaa
     1921 caactgattt aaccgggtat caatttgcgt caacagcgtt ggcgaaacct ctccggtctg
     1981 tttttgctct tccagttgcc gaagcagttc ttccagcggc ggctccttta acgactgccg
     2041 gatgccgtac accgccgttt ttagttccga accggtcaga tatctcccct tacgttcatc
     2101 cagtgcatcc agctgcgccg acaggcgctg tagctggctc tgcgcctgag catagccatt
     2161 cagtgcctca gcagacaggg cgctcagctc ccgctgtttg cgccactgcg cgttcagcgc
     2221 cctgacctga ggattatccg gccacagggc atcaagctgg cgtagctggc gatatccctg
     2281 ctccagtggc cagcgggcag gcaatttgtt taactgctcc agttgcatct gacttgccgc
     2341 aattgttctg tcctgagcca gcagcgcctt atcctttaca tccagctttg ccagttcgcc
     2401 gctctccagc gacagcggca acggcatgac agacagttgt attcgctgga ttagcgcgtc
     2461 cggctgatta agctgcgacc agccccataa tcctgttccg ccgagaccgg ccatcacaac
     2521 cattccggtc ataaatatcg gccatgtccg ctgccgggag atttcagggg atggaacggg
     2581 tggactggca tccttatcgt tttgctgaac ggatctggtt gcaaaaaccg gctgtggact
     2641 ttcctctgac tttttcggtt gattcatttc ccggcggggt aattcagggg gagtgatagt
     2701 ggtttccgct ccctgaggcg ccagccgttc cagacgcaat gcggtattgc gaaactgccc
     2761 ggccagtaga tccagtttcg tcatatgcca cagttcacac tgttgcagcg tggtttcgat
     2821 ggcggaaaga tgctgtacac aacggtagat ctgcggcaga tcctgatact gaatatccag
     2881 cgttctcagt gcctgctgca ttttttctgt gacccagctg agcagtgata ttctggaatg
     2941 tacctgtacc ggccagaagt cagcccagcg ctggagcagt atatccagtg aaccgagtcc
     3001 gtctgccatc cctgccagcc cctgccgtct ggttatcgcc agtacgtaac agaccagcgt
     3061 ctgtagttcc acgccatttt gccggaacag cgcgaggcag agtttttcca cctgacgcca
     3121 gtccacatcc ggtcgggccg ggtgagtcag tttactcatt tcagcctgca gttcaccgga
     3181 ttccttataa gtacagggat cgccgcccat gacgaactgg ctgataaaat tcgcgttact
     3241 tgccatcagt acgcccgctc ctgttgtttg agatgacgca gaacaattcg tccttccgga
     3301 gaaaatacgc catcaaagcg tatccagccg gtatcgggaa gctgcgcgtg gatcaggtaa
     3361 ttaagatgcc ccggacgggg ctgggggagt aattccacct gtatctgact caggcgtggt
     3421 tcataacgca gcagtgtttc ttcaatctga cgcatcaggc catgaatacc cgcagccatc
     3481 ccctgatgga tcagactgag atccggcaga ccgtaatccg gcaaatgcgc cagcgtgcct
     3541 gcccggcagt tgataatgcg ttgcatattg tccatcacgg aaaggataag ctgatcccgc
     3601 tcgctgatct gttccagcgg taactcgccg ctgaaactga acgtcagcat ttcatataag
     3661 gaaggtgtcg acatcggtta tttatccttg agaggcagca gtcgcaggtc gcttctcatc
     3721 agttcaatcg agcgcggctt gtcggcctcc agttcgtccc ttttgatcac cagacgccag
     3781 ctgtcgcttt tttcatcagg gtgataaaac tgcccaataa tggcgacaaa ctgcgtctct
     3841 ttatccagcg gcacattcag ggatgccgtg ccttccggtt ttacccagac ggtgtgtttt
     3901 gccagtacgt ccttctccag cgtcttctct tcctgcataa acagggtgtc gtaatccgcc
     3961 ttgtcaaaat cttcgcgggt tttcagttgc cagacatgca ccatcgtgga aagcggcgta
     4021 tcttccgcat ccgtattcag ggcgctgcgg gagaagaaat cgagatgcag tattttgatt
     4081 tgtttgtaga aaacggctga ggccacgctt ttcgattcat ccaccacttt ctgaatcagc
     4141 ccgcagccac tcagaacaac cgtcagcatc gccatacaga gcaacgccgg acgtttaaaa
     4201 cgaataggaa acatgagtca tactctccct gttacgaatg gaaaaagcgg gtattaatcc
     4261 ctgataacgc cccagactga ccgtcactgt tccggtcgcg ggcgcggcaa aggaggcgcg
     4321 aaggatgccg gtacggctaa tctgtacacg ctgttttccc agccgggcag caggcaacag
     4381 gctgacgggc agcgtcagtt gcagacgggc ctgacagcgc cagcccagat aaacgcgcag
     4441 cagcaccagc aaatcgctgt gcagttgccc gtcgggcagc cagccacgtg cttctgcggc
     4501 atcttcggta tacagtttca gcagcaactg gctgttgacg tcggtaccgg tacgccccag
     4561 cagcgttcgc gttttcaggc tgatgcgtga attttttgac agacccgcag gggccgggag
     4621 aattacaggc tgcggatcgt gaggcaccac cgtcgctttg gtcagcgggg caagcagccc
     4681 taccagtgcc gtgacgcctt ccgccgtgcg cgtcggcagg cgcatcacgc tgagcagggc
     4741 cagaaaacgg gataccggcg tggcgatatg gttctcgctg ccgggaatac ccagcccgat
     4801 gagtcccagc agacaacggg aaatgtcatc cacggcacct gcttcaaacg acgccgggta
     4861 gttatatttg cgccagatgc ggtagtactg agtaataaag cgatggttaa aaatatcgag
     4921 aaacgccatc actgcgtcat gtccgtcatg acgctgggta atgtagtcca gataggcggt
     4981 tggcagcggc gaatccacgc cgtacaggcc gagaaaagtg gtgcgcaccg tggggggcgc
     5041 gtcgggattg cccacgtcac gttcaacgtt ttttaactcg ctgaccggaa agcccatacc
     5101 tggatgagga cgaaagcgca ccgcatcggt ggcggggctg tcggtactgc ccagcggcgc
     5161 gttctgctgg ctctgttcca gcaactggca aaaacggtaa aaattgattc gcgcaatatc
     5221 cggggccagc cggtcggtca gcccggtacg cgctggctgt gatgctctgt ccattgcagg
     5281 cactttcctg tgggttgcag aattaaggtc agttgagtga ataagtggat atcggcatac
     5341 agtgcgaaaa agcggtgcag cagttcacca aacagcgtga tatcaccttc tccggcaaag
     5401 ccgttactgt ccagcgtcac ctgaatatcc acgccacgca gcagaaagcc tctttcaaaa
     5461 cgctgtatca ggctgtgctg aacgtcgaca atcgcctcca gacgacggcg gttcatctcg
     5521 ctttcggtcc agtcgtacag tgccagcgtg ccgcgcagaa tttcggcgtt atccatcatc
     5581 gataaaaagt tagaaccaag gtgactgagc acccgccagt ggaagcggtc acgcgcaggg
     5641 ggatagcagg gctgcgtcgg agcgcacagg ttgcgcaccc gcaggacgtt ctgtgaggca
     5701 tgaactggcg tatcgagcag cgtgctctgt aaagccttac ggggaagctg cccgttagta
     5761 ccggtcaggt ttaaagagag cgtttcatcc tccagcatcc ggtcagtatc aaacgcatcg
     5821 ccgcccagaa tcagccaggt gtcatgcagg ccggaagggc cgcgctttac ccgcgtgtgg
     5881 taatagcgtt ccggcgcatc gtggcgcagc atcccgcccc ggtgacgaaa gctggagaag
     5941 gggacatacg cctgactgcc ggtatggcgc gaagaaataa tgttgtcgac ggaataaatc
     6001 tcggtatgcc cgtcctgaat gcgcatcggg cgcagcagaa attcgttttc cagcggcgaa
     6061 aggtgtaacg gatcggcttc gagcgggaac aggttgatga ccggcacgca gtgcaggcgg
     6121 agattgtcgg tattaaacgg caaatcatga ggccagtttt ccgccagcac cacgtcaatc
     6181 tcaaagccgg ttatcctttc cggccagatg acgttctcca gcccgttcag cgccacaaac
     6241 ataaacttct cacggaaggt gaaatattcc agcaacaact ggtagccgct gaacgccgac
     6301 tcgcctttcg gccacagtcg gtcatcgtca tcaaaaccca tcggggaaaa gtgggcgtcc
     6361 agcacacggc gttcctgccc cggcagacgc agccagaact gctggatgcc aagcgttaaa
     6421 gcccggtgca gcgcacaggc caccgggctg tcggcattga gatagagcgg caggcgggat
     6481 aaatcgatct gtgaccagtc accaaccagc gggccgcatt caaagcgcag acggataacc
     6541 gagcgtccgt ccggttcata ttgcaatgac acgtccggca acgccagcgg atgcagcgtg
     6601 atatcacgcg tggcgctgta gacgcagcgt gttctgcgtt cgccgatggg acgggatagc
     6661 acctgaaact ctttcagcgt atcactttgc ttcatttgct ggtgatccgt ggtcagttcg
     6721 accacggaaa gcgacggaat ggtgcgcagg tagtgcggcc acagcaggct gaccagcccc
     6781 tcggtcagtt cgggcaggtc gtcgtccagc ttttcgcgca gtcgccccat taaaaaagcg
     6841 aagccttcga acaggcgctc cacataggga tcgcgtgcgc cgggtttatc aagattgagc
     6901 atcgcggcgc ggtcggggta agcctgcgca aattctttcc ccgcttcacg caggtagcgc
     6961 atttcggctt cgtagtagcg ttgggtcagg tcgtccatcg gaggatttat cttcctttta
     7021 ttgaaattaa taccaacgag tagtgagatt agaatttttt attttaagaa tcctaaggac
     7081 atatagatgg atcatttatt tcaaaagaaa aatcggactc catactagat gaggatttct
     7141 tcaataaaga ggagatatga tatccagata aagttttaga attattacca tcagatatca
     7201 aaatgctaag ttcagacaca atattcacag ggtttttatt aaaaacatct gactctctac
     7261 ctgtgtaaat aaagtataca attggctttc gctctccgac atcaagatgg aaattctctc
     7321 ttactttacc atcataatgt gttgaattat taactttcga aatactaata gatactttgc
     7381 atgtatttat catgacaatg gaacaagaat ggaatggttg agataaacat ccagcacaca
     7441 gtgtgcataa taaaaaagag aatgttaatc taatcatcaa atttcacttg atagtatcaa
     7501 aaaatcacaa atgattaata atgtcaattt ttcagttgat tatgatatga atcatactat
     7561 gggcaaatat cctgactatc tatcatgtac tttacaaccc catgcttctt atcgtctttt
     7621 gttgaaatgt ccctggataa ttcaattaga tcgtttttcc caaatttttt tcacgtcctg
     7681 atgataagat aacaaacgga taatcaaaat tacccgagct atctttattt gcggtgaatg
     7741 cttcttcata ttggaaacca tcattgatgt tttcagtata aaaataagac aaaacatcaa
     7801 tcccattaac aggtaagtaa atatgatgtt gcttaataac attacctcca aaaacatact
     7861 caggaataaa cacattaata tctgaggaac atacattcct aatcctgacg acattggaat
     7921 acttcatttg cgaagtacat ccggcaagaa atattaaagc aataactact ctagtcatca
     7981 aattttatcc tgcttaacca gatcaatccc tgaacatctt catctgctgc aaccaatcga
     8041 gtatacatgt caggacgata ttttccatat ttcatcacat caatctctcc ctgagcgggc
     8101 ataacattcc ctggtattgt tttttgcata agagtagatg ttcctttatg agtccacgaa
     8161 taatcagggc tagaaccctc tccatattca tccagtactt tatgccccat ttcatgagca
     8221 ctatcccatt tatatctgtt attagcgtca agaagcgcct gtaattcatc ttgaatatcc
     8281 ttataactat caccgtagtt ataatatatt ttttcaaatc ctgatagact aacggatgcc
     8341 tgaaagtcag gatctaatga tgatatcaaa gaaaaagttc gcatggatgg agtaatattt
     8401 atctcagcct ttacatttac tttaaaaaca ccaatcgctg taacaatatt ttcaccaatc
     8461 cccccgccgc gacttccatt tcgagaccaa taaaattcaa ctccattttt ggtcaggtta
     8521 accaagtcgt tataaggaac tggggataac ttaggattac ttccttttat ccctccatca
     8581 ctaactgcca gtcgccatat tatatctact gttttttgtt ttctgtctac ctttacatcg
     8641 acccattttt gttcctctgg tgaattatga agtggataat caacttttat catcatatca
     8701 ccacataaag caatcagtct tacaagtagt gatttgcttt ttagcttagt tgtatccagt
     8761 atgccttgat tactgtagcc atcccattgc caggcatgct ctcccttatc acaatattta
     8821 ccggtctgcc gttcactata aatcacttca ttaccatcaa gcacttccag agaaagcgct
     8881 tcagcctcat cgcctttatt tgtgatggta aaatctattt tttctgtatt cttcgtacta
     8941 ccaccgctaa tcatttcttt ataatcaggt atattcagtg gcgtcagtat cttattacct
     9001 gataatttaa ttctgtaatc cagttttaca tcatcagagg atgaatcaga accagaagtc
     9061 agatcctttt tcgccgactg cgcatgttgt tccgcttcac tttccccgtc atccgtctca
     9121 tacgtatcat ccatcattga cgcaataaac cgtgctttac acgggcaact gctctgactg
     9181 tccagcgtcc ccgccatcag cctgccgtga atatctgttt caggaacgcc gcccacaata
     9241 ataaacattc ccggatatct gccgcaggtg acccggtcca tatcccgcgc ctgcgggata
     9301 cccatgatcg tatggtcgtc cgccccttca ataattttgc cgccgcaggt ggtcttatcg
     9361 cccctgacca gaaaataacc tcttcccatc gtcatcactc cttatgctgc gtatcacaca
     9421 gtaccgccgc ccgcaccgga ttgattgcca ccagtgtacc cagcagggca tccatttttc
     9481 ggttcagcag ggctttatcc gcatagcggt gcgcccgcag acgcagcagt ttcagttgtc
     9541 gggcttttac ctcaaacagc agttccggct cccactgcgt cagcgttatt ccctgtgcgg
     9601 cagtatccag ttcttccagc agcaggagcg ccatctcgtt tttaccgtac tgttccgcca
     9661 cccgtgccat cagcagccgc aataacaggc gctgtcgtgc cgtggtgatg cctggacggg
     9721 tctgtatcca gcccagtgcc gcttccacgc cctgactgtc tgccatctcc agcgcttccg
     9781 gctccagcgc cagtacgtcg tttgtggcgt cgtctgtcgg cacagcagct ttccccgcaa
     9841 gccatgcgcc gtcttcccgc atcatgacct gctgcgctat ccagttgcgc gtcacctcgt
     9901 cggcgaacgg tgtgccgtcg ttccatgcca gattttccag ccctggcagg cgttccagca
     9961 gcagcgccag atcctgacgg ataaccgcag tccatctgtc ccacggagca cccgccttcg
    10021 ccagcgcctg atgcagatac cactggatat ccagccagaa atggctgaca ccggtgctga
    10081 acatcaggtc ggcctgctcc agcagttccg tccagttctg ctgggcatac agccgcttaa
    10141 gctggttgcg tgattcggtg cgcggcgggg ccagccgggt tttgccatcc acatccggcg
    10201 gcagttcatg cacagtgtcc cagcgcaggg ttttcatcag ccggtgtgct gaaagccagc
    10261 cctgcggctg ttcgttcaga taacgggcca gcactttcgc ctggtcgagc aggtcgcgcc
    10321 cggaagtgat ggtctgtacc tgtggcgcat ccaccgggga agacggtgca ggaacgctgc
    10381 tgctgttttg tggcaccacc gcgttcatcc cacccgactg cgccagacgt gattccagcg
    10441 cgttaatcag cggcatcagg gagggggaat gctggtcttc gggccatgca gtgaaggaaa
    10501 ccgtcagttg attaagagcg gcaacaatgt tcgcaaaatc ctcttttgcc acttccgggt
    10561 agcgggcaag gctgtcgagc attttttcac ctgccagcca ctccagcgcc atttttcggc
    10621 ttgccggacg cgagggcagc agttgcgtac cgaagcgttc caccagcccg gctaacagcg
    10681 ccagcccctc agccagtccg cgctcaccgt cgcggtgcag tcgcgcccag atgtagtaag
    10741 tggcgatgcg cacgtctttt gcctgcgtca gcagcaggga ttcggcaagc tggcagatga
    10801 ggccggtatc cgcgccggaa agtttgttca tttcgtcgcg gatacgcagg aaatcgtcgt
    10861 ggtagccggg gtcttccccg accggcgtat ctgccgtcac cggcagcagc cacgtctgcc
    10921 acagtgccgc ctgtgttcgc gccacatcca gtgcgtcccg gttggcaaaa cagctacggg
    10981 tcagcttttg caacagttca ctcatcggcg tcttccccat attcttccgc atccgtcata
    11041 ctcctgccgg tcagaaatac ctgccccggc aagcggaagt ttttcagttc cagcagcgcc
    11101 agcggccctt tgccctgttc aacccgcagc aggtaattca gcggcaggcc gtcctgcgct
    11161 ttccacacca ccttaaaggt gctgctgtcc accggcgtca cctgcgcctg ttccagcagg
    11221 cgaataaggc tccagcttcc ccggtaatcc gccagtatgc gttccatcgc ctgtgtgctg
    11281 gtccagctca gcgatacgcc gggttgccac tgttcgtccg gccagttaaa acgctgccag
    11341 cgttctttct ggttgaaata ttccagttcc tgcccgtcaa tcaccagatg tgttttcatc
    11401 acgtcacggg ccggttttgc ccgcagttca aaatgtattc cggcatcgcc gctggcaaag
    11461 gcggtatcgg ccacgtcgcc caggcggttc agggcgcgga taaagtccgg gttaaccgtc
    11521 agcccctgcg tgttcatggc gtcagccacc cagtagcggc cttcacgttt cagtacgccg
    11581 gaaaggttgt tggcaataaa ctggttgatg cgcccgtcat cgcgcaggta ctgcgccagc
    11641 agggggaggg atgcatcgtt ttgtgaggct ttgaacgggt agcgaccggc gaagtcctga
    11701 tcccagagtc tgacaatcgc ccgctgccac tggcggttca ggctgtccgc cgcaggcgtc
    11761 agcacctgcc gccacgactg ctctaccggg cgaacgaaca gcgcctgacc gaagccactc
    11821 cattcctcgc ccagactggc ggcgattaac cgtccgtagt cgcgggtgtc tgtgaggtca
    11881 acggttttac cctgtaagac cgtctgcgcc agctgttggg tcatctcctg cgggtcgggg
    11941 gcgttggtca cctgttgcag tttgagacgc acgcgggtga cgcgggtgag gtaggtctgt
    12001 agactcagct ggctgtcgcc gcctccggcc ttatcgccca gcaaccgcag cagcggcgcg
    12061 aaggttttat ccagcggccc gacaggcacg tcgtcacctt cacgggattg ctgcggcgtt
    12121 ttctccttgc cgttaaacag ttcctgtgcc gatttcgcca gcgagtctga cagaccacgg
    12181 ttttccctgc ctgccgccgc ctgccacgcg agggtgtccg tcagcgcaat cagcggcgac
    12241 tgacgggcat cggccatcag tgtcagctgg tcgagaatgc cggagagtga gtcttccttt
    12301 ttccagcgaa tgctgttgag aaacgccagc cagcttccgg cgaagtcggt aaagtagcga
    12361 gaggtgagac ggttacgcag cgtatccggc gagatatccg cagaggtatc ctgctggcgg
    12421 tcgctgagta cccagtcgat ttcctcgcgc cgcgccgtca ccacctgctc gatggcttcc
    12481 ctgacctgtc cttcccacgc ctgacgggtg aacatccccg gcaccgtctg ttctgtactg
    12541 aaaagagatt cggtgagggt atccccggtc atgtccgcca gcgtcatatc ggcgtagttg
    12601 cgggacacct gttgcagcac gttctggtag agggtgtttt cggcattacg cacaccaatc
    12661 tggcgcagca ggattttacg taccgcgccg gtcagttccg gcggcggcgc ggttttccac
    12721 tgcgggtgtt ccggcaggtt cgccatccag aaggtcagca gcgacggcaa cacgctctgc
    12781 cgcacgcctt ccgggatatt ctcgtagcgc aggccgtctg ccatcagcgt ggtactgaaa
    12841 aaggcagcgt cggccttttc cgggcgggaa gtcatcagca atgccttaag ctgcttatag
    12901 cgttgttcac cggtggcggt acgctgagga ctgttgggcg gcagcgcgac aaaggcgttc
    12961 agttgctgtt gcagatggtc aacggccacg tcgcgcacca gccggtttgc cgcctgcgca
    13021 tagccgggaa aggcggcagc gagcagttgc tggttacgtt caaggccaaa acgctgatac
    13081 cacggcgcac cctcacggat acggtattgc aggcgctcca gttcgccctg cagggtatgc
    13141 agtgccacca gttgttctgc cagcggcagg cgggtatcga gcgcacgcgc cgtctggatg
    13201 ctggtttcct gaacaagcgc gcggttggcg aagaacgagg tcgtcattcc ggcccccata
    13261 tcaccagcac gcagacagcg gacatcagtg cggtacgcag ccacggaaaa cccaccgtgc
    13321 gcccgcgcgc gttatcaccc gtcacgcccg cccagaccgg tgacggcagc cacgcatgag
    13381 gtgcggcctc cggcacggcg gcaagcggcg ggctgaacat caggccacgc aggcgcaggg
    13441 aagaaaatgc gccgcctgcc agcagcggtt tcagaacgct ttgccagtga gcgatacccc
    13501 gctctgcaag ggtatgggcc agacgcagca ggaaatcatg gcggttattg gtcagcagtt
    13561 gcgacatgcc ctgttccgtc agcggcaggg tttgtgcttc cagcattaca gccagttgtt
    13621 ccggggtaca gcgttccggc agcaggcagc cgacgctttg cagggggcgt ccggtctgat
    13681 aatcaccgtc gtcacagacc tgccacaaat agagtggagc ctgaaaaccg agtcgcttac
    13741 cgccgtttat cagtccgcgc cagcctttgt cgagttgtgc ggtctggcgg ctctgttctt
    13801 ctgtcagcgg ccagatgatg ccgtccagcg gacggctgcg gcgcagtttt tttaaggcgg
    13861 tcagtagcgc gacatcaggc tccgctgatg gcctgccgcc gtatatcagc accgtgtggt
    13921 cgccctccag ccagtgttgc ccggtcagcc ccggcgcgat ggcctctgcc tcttcaggct
    13981 cgccggtcac cagcagcagg cggactttac gccgccagaa tgggccgtag cggtcgcgca
    14041 ggaaggcggc gagttccacc attgccggat gacggctgtc ggctttcttt gtcgtcaccg
    14101 cacgatagtc atctccggcg gcaagctgtc tgcggagttc aaagttctgc acggcagcgt
    14161 ggcggttcag cagatgataa acccccatga taatcagaaa gaccacgcct gcggcaatca
    14221 cgccagccat ccagtacatt tgctgtgggc tacccggttc aacgcccagc aatgccggtt
    14281 tacggtgaat aaacgtgcaa agcgcaacca caacagccac ggtcatgacg atggcaggcc
    14341 ctgaaaccga acggggccgg agacggttta atcccggaac cactatgatg cctcctgagg
    14401 ggaaacgaat ggcgacacca cggtcgccca tgaataatgc ccgccctgaa cgccgctgag
    14461 cgtcagttgg gggtgttgtg attgtattgc cgccagcgtt gccacagcga cggacagcca
    14521 cggcgcagcc ggtccggagc gccccagaaa ggcatccagc tcgtacatca gggcttcctg
    14581 agcaaacggc tggttcaggc ttaacaacgc ggcacgctgt tccctgtcca gttcggcagt
    14641 ccacataccg gatatggcat ctggctgcac cggcatccag tccagcgcct gtgcaatgcc
    14701 tgacgccatc atctctgtat ctgtgatacg ttcaggacga tgcagcaggg ccagcggcgt
    14761 cagcgcggtt tgcgtcagtc ggtttgccag caacagcgcg gtggcggatt ctgccgtccg
    14821 ttccgggttc ttcggttcaa gtcgtacgga aatcaccagc agtacggcct tttcctgaac
    14881 aaagttgtcc agccagtcgt ccacggcggc cagccccggt tccggggcag aaaacagacg
    14941 ggcttccggg agtccggctt tttgccattc gtcctgccag atagttctca cggaggcccc
    15001 tgaaagcggc gcatcaattt ccagtagtac gttgatatga aaatcagggg gcagcagaaa
    15061 caattgcggt ctgatatcaa taaaaagacg ggaaataatt tcggataaac gcccggtaac
    15121 cggtttattg gttaatacag gaatgacact gcgacgcacg gtatcttctc cgcgcaaatc
    15181 agcgtaagta ttcaacacct gctgacgcgc cagaaatgct gttgtagttt ctgcaacgga
    15241 atcaccattc agcgcggttt gtacggaaaa actaagtatc tgcaacgccc gtcgtccccg
    15301 tcgcgtttca tgcaaaatca cctcttcccg gcgcttattc caggcatcag cccgaatgta
    15361 ctgcgtgata aaaaacagcc agcgaactga agcaaccagc gaccagataa aggcaggcaa
    15421 accgcaggcc agcccccaga aatgcgggcc gttttttgcc agttcatcgc tggcgaataa
    15481 tgaagtcagg agcacgccca cagacagcat cagtaataag gctgacaacc atttaatgtg
    15541 ggctggcggt gcgggatagg gttcagcatc aggaatagtg ttaagtcgga caggcattgt
    15601 aataatccat tattctgaaa tataaatgta atgaatatca ggatgtttaa actatgtaag
    15661 taattcacta
//
LOCUS       DAZFRE010000105        15561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFRE010000105 DAZFRE010000000
VERSION     DAZFRE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.105"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            54..620
                     /gene="yceJ"
                     /locus_tag="ACHKUI_004029"
     CDS             54..620
                     /gene="yceJ"
                     /locus_tag="ACHKUI_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6600120.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGTQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKYDTLKRMLGKSSSDYGV"
     gene            624..1199
                     /gene="yceI"
                     /locus_tag="ACHKUI_004030"
     CDS             624..1199
                     /gene="yceI"
                     /locus_tag="ACHKUI_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6600121.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1241..2293)
                     /gene="trhO"
                     /locus_tag="ACHKUI_004031"
     CDS             complement(1241..2293)
                     /gene="trhO"
                     /locus_tag="ACHKUI_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6600122.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPDEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2518..3438
                     /gene="lpxL"
                     /locus_tag="ACHKUI_004032"
     CDS             2518..3438
                     /gene="lpxL"
                     /locus_tag="ACHKUI_004032"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6600123.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3610..4836
                     /gene="mdtG"
                     /locus_tag="ACHKUI_004033"
     CDS             3610..4836
                     /gene="mdtG"
                     /locus_tag="ACHKUI_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO6600124.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            4919..5293
                     /gene="msyB"
                     /locus_tag="ACHKUI_004034"
     CDS             4919..5293
                     /gene="msyB"
                     /locus_tag="ACHKUI_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFO6600125.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5294..5521)
                     /gene="yceK"
                     /locus_tag="ACHKUI_004035"
     CDS             complement(5294..5521)
                     /gene="yceK"
                     /locus_tag="ACHKUI_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6600126.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDVHHGPYE"
     gene            complement(5693..8236)
                     /gene="mdoH"
                     /locus_tag="ACHKUI_004036"
     CDS             complement(5693..8236)
                     /gene="mdoH"
                     /locus_tag="ACHKUI_004036"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6600127.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEVKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8229..9764)
                     /gene="mdoG"
                     /locus_tag="ACHKUI_004037"
     CDS             complement(8229..9764)
                     /gene="mdoG"
                     /locus_tag="ACHKUI_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6600128.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10158..11315
                     /gene="mdoC"
                     /locus_tag="ACHKUI_004038"
     CDS             10158..11315
                     /gene="mdoC"
                     /locus_tag="ACHKUI_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6600129.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11323..12804)
                     /gene="clsC"
                     /locus_tag="ACHKUI_004039"
     CDS             complement(11323..12804)
                     /gene="clsC"
                     /locus_tag="ACHKUI_004039"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6600130.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPQLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFIHHLVDGTLSLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(12746..13279)
                     /gene="ymdB"
                     /locus_tag="ACHKUI_004040"
     CDS             complement(12746..13279)
                     /gene="ymdB"
                     /locus_tag="ACHKUI_004040"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6600131.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13374..13685)
                     /gene="ymdA"
                     /locus_tag="ACHKUI_004041"
     CDS             complement(13374..13685)
                     /gene="ymdA"
                     /locus_tag="ACHKUI_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6600132.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSAVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13806..14138)
                     /gene="csgC"
                     /locus_tag="ACHKUI_004042"
     CDS             complement(13806..14138)
                     /gene="csgC"
                     /locus_tag="ACHKUI_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6600133.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNIPPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14197..14655)
                     /gene="csgA"
                     /locus_tag="ACHKUI_004043"
     CDS             complement(14197..14655)
                     /gene="csgA"
                     /locus_tag="ACHKUI_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6600134.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14696..15151)
                     /gene="csgB"
                     /locus_tag="ACHKUI_004044"
     CDS             complement(14696..15151)
                     /gene="csgB"
                     /locus_tag="ACHKUI_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6600135.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3884 a         3989 c         3667 g         4021 t
ORIGIN      
        1 cattctccga cacgccattt tctttctctc gcaaattcgt tttggatgca cttatgtcat
       61 tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa
      121 ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg
      181 gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc
      241 tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt
      301 cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg
      361 ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg
      421 gctggtttga cgtccccgcg accctcgccg acgctggcac acaggctgac tttgccggtg
      481 ccctgcattt ttggctcgcg tggagcgtcg tcgtactgtc cgttatgcac ggatttatgg
      541 ctctgaagca tcatttcatc gataaatacg acactctgaa gcgcatgctg ggaaagtcgt
      601 catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcttcct
      661 tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc
      721 acgcctttgt taacttccgc atccagcatc ttggctatag ctggttatat ggcaccttta
      781 aagatttcga cggtactttt acctttgatg aaaaaaatcc agctgccgat aaagtgaatg
      841 tgacaattaa caccaccagc gtcgatacta atcacgccga acgcgataaa catcttcgca
      901 gtgcggattt cctcaatacc gcaaaatatc cacaggcaac attcacctcc accagcgtga
      961 agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggt gtaaccaagc
     1021 ccgtcacact ggaagcgaaa ttaattggtc agggtgacga cccgtggggc ggtaaacgtg
     1081 caggctttga ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag
     1141 gtccagcgtc tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag
     1201 agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa
     1261 tgcccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac
     1321 gtcctgcccg acggcgtcgc tgttcatctg gcggtaacgc gctttcttcg cagcaaatct
     1381 cactacaaca acctttgtat ttttccgcac atactggaca ctgaataaac agcagatggc
     1441 agccatcatt tttacagttg gtatggctgt cgcacggcgc accgcactgg tggcagtgcg
     1501 cgataatctc atcagatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa
     1561 taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct
     1621 cgatatgcca gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac
     1681 gaatgccgcc ggtgcagtac atgacaattt ttttatcttt atgtgcctgc atcatctcaa
     1741 ctgcttttgg cagctgctca cggaaggtat ctgccggtat ttccagcgcg ttttcaaagt
     1801 gccccacttc atactcatag tggttacgca tgtcgataaa caatgcatcg ggatcgtcaa
     1861 gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag
     1921 gatcgtcaat accgtcggca acaatgcgat cgcgtacctt catacgcagt acccagaagg
     1981 atttcccgtc atcatccaac gcgatgttca ggcgtaaacc ctctaaagcc gggtcgaagg
     2041 catagagctg cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa
     2101 tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac agttgatata
     2161 aagcgtcacg ggtcacctta ggatcggcaa tgtggaaata cttataaaac gaaatggtgg
     2221 ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt
     2281 gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat
     2341 atagcaatta tcgataatta acatccacac attttacgct acattttagc attaaaaatt
     2401 atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gtcacaaatt ttggttgcgg
     2461 acgaaaaaat gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg
     2521 acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg
     2581 ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc cggttatcta ccgcctcggt
     2641 tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc
     2701 aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag
     2761 aatttcgaat ccgttggcat gggcctgatg gaaaccggca tggcgtggtt ctggccggac
     2821 cgccgaatcg cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg
     2881 caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct gggtgcgcgg
     2941 cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt
     3001 gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat
     3061 ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accggatcat
     3121 gattacggcc cgcgctcaag cgttttcgtc ccgttgtttg ccgttgagca ggctgcgacc
     3181 acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca
     3241 cgccgtaagc cagatggcaa agggtatcag ttgattatac tgccgccaga gtgttctccg
     3301 ccgctggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc
     3361 atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc
     3421 gttccttcac gctattaaat cttccatgct ggatgcttca gaatggcatc cggcattacc
     3481 acagaaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg
     3541 gcgcataatt agtgtgctta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag
     3601 cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc
     3661 gtcgcctggc taggctgttt tcttaccggt gccgccttca gcctggtaat gcccttctta
     3721 cccctctacg ttgagcagct tggcgttacc ggccactccg ccctgaatat gtggtccggt
     3781 attgtcttca gcattacatt tttattttcg gccatcgcct caccgttttg gggtggactc
     3841 gccgaccgta aaggccgaaa actcatgcta ttacgctctg ctctcggcat gggcatcgtg
     3901 atggtgttga tgggactggc acaaaatatc tggcagtttt tgatcctgcg ggcgcttctt
     3961 ggtttacttg gcggatttgt ccccaacgct aatgctctta tcgccacaca agtaccgcgt
     4021 aataaaagcg gctgggcgct gggtacgctc tccacaggcg gcgttagcgg cgcgttgctc
     4081 ggcccaatgg ctggcggttt gctcgccgat agctacggct tacgtccggt attctttatt
     4141 accgccagtg tgctcatact ctgctttttc gtcaccctgt tttgcatcag agaaaaattc
     4201 cagccggtca gcaaaaaaga gatgctgcat atgcgggaag tggtgacatc acttaaaaac
     4261 ccgaaactgg tactcagcct gtttgtcacc acgttaatca tccaggtggc gacgggctca
     4321 attgccccca ttctgacgct gtatgtccgc gaactggcgg gtaacgtcag taacgtcgct
     4381 tttatcagtg gcatgatcgc ctcggtgcca ggcgtggcgg ctctgctaag tgcaccacga
     4441 ctcggcaaac ttggcgatcg aatcggcccc gaaaagatcc tgattacggc gctgatcttt
     4501 tctgtactgc tgttgatccc aatgtcttac gttcagacgc cattacaact tgggatttta
     4561 cgttttttgc tcggtgccgc cgatggtgca ttactccccg ccgtacagac actgttggtt
     4621 tacaactcga gcaaccagat cgccgggcgt atcttcagct ataaccaatc gtttcgtgat
     4681 attggcaacg ttaccggacc attgatggga gcagcgattt cagcgaacta cggtttcagg
     4741 gcggtatttc tcgtcaccgc tggcgtagtg ttattcaacg cagtctattc atggaacagt
     4801 ctacgtcgtc gtcgaatccc ccagatatcg aactgatttt tcgcctttca tacttgcaaa
     4861 agcggagaat cagctatcct tttccctgaa acctcatcaa ctcaaaggga gaatcgtgat
     4921 gaccatgtac gcaacgcttg aagaagccat tgacgctgcc cgcgaagaat ttcttgcaga
     4981 caaccccggc atcgacgccg aaaacgcgaa tgtgcaacag ttcaatgccc aaaaatacgt
     5041 tttgcaggac ggcgacatca tgtggcaagt tgagtttttt gccgacgaag gggaagaagg
     5101 tgaatgttta cccatgctta gcggtgaagc tgcgcaaagt gtctttgacg gcgactatga
     5161 tgagatagag atccgccagg agtggcaaga tgagaataca ttacatgaat gggacgaggg
     5221 ggaatttcag cttgagccac cgctggatac cgaggaagga cgcactgcag ctgatgagtg
     5281 ggatgaacgt taatcactca tacgggccat gatgtacgtc gatcggcagc agtaaagtat
     5341 cgaaaaccag ggagaatggc agatcaagga tcgtgacgta acgccaggcg gagtcacgca
     5401 cgtcccattg cacaccggga taatattggt tgccatgccc ctgccccggt atagtgcgac
     5461 taatgatgct gccacagcca ctcagcaagg tcaccattat gctcatcaca attaaacgca
     5521 tttgatacct cttccgtaaa aaataccggc ataacgccgg tatttttatt ggctcgttgt
     5581 ttttgccgga tgcggcgtga acgctttacc cgacctacaa aatcttgcaa aatcaataaa
     5641 ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag gcaactacgt ttttattgcg
     5701 aagccgcatc cggtttacgc aatgccagtg gattgagctt tatcccttcg taataactca
     5761 cccatgaaga atatctctcc ggggaattcc agacgcggaa atgcagacgg gccatcgtca
     5821 ccggatcgct tagcagcacc aggcgacgat cgcgattcag cttctctggc gtctcgttca
     5881 gcgcctgttc aacgtggcgg tcacgggcga tttccagcac cttgctggcg cggtgacgcg
     5941 cggtcgccat tgcggttgcc agagcgttaa atgacgggtt aaacaccgcg tgcataaaac
     6001 catcatcaag ggagcgttga cgattcatct caaggaaccg atcggtatca accagcacct
     6061 gcggcggtga atactcttcc gggatcagga acagtttcca gcgtttggtt cgcagaccaa
     6121 cggtggcacg gctggaaata accgaaacaa acggtgacag gatcaacgag aagacaatcg
     6181 gtgccagcca gaacagaaaa cgcagatcca gccacgccat cccaacagcc cacactaacc
     6241 ctaacagcag ctgtgagccg tggcgtttga acgcttcccc ccaggaagtg gagtcatcat
     6301 cacgctgcgg cgaattccac actacttccc agccaaggaa cgcgctgacg acgaagaccg
     6361 tatggaacaa catgcgaacc ggagccagca gcacggaaaa cagcacttcc agcagcaacg
     6421 ataatgtaac gcgccagaag ccgccgtatt ctttcgttcc tttgcaccag ataagcaaaa
     6481 tgctcaatag cttcggcagg aacaacagca ccatggtcga agcaaaaagt gcaatcgcca
     6541 gctcaggacg ccactgcggc cataccggga acaactgccg tggttgcagg aagtattgcg
     6601 gttcggtcaa cgcatgtact acctgcaatg cagtagagag cgcgaggaac ataaaccaca
     6661 gcggagcgga gagataagac atcacgcccg tcaggaacac cgcacggtga accgggtgca
     6721 tacccttcac caggaacaga cggaagttca tcaggttacc gtggcaccag cggcggtcac
     6781 gttttagctc atcaagcaag ttaggcggta attcttcata agaacccggg agatcgtaag
     6841 caatccagac cccccaacct gcacggcgca tcaacgccgc ttccacgaag tcatgtgaca
     6901 ggattgaacc ggcaaaagaa ccttcgcccg gcagcggagc cagtgcacag tgctcgataa
     6961 acggtttcac gcggataatc gcgttatgcc cccagtagtg cgactcgcca agttgccaga
     7021 agtgcaaacc ggctgtaaac agtggcccat acacgcgggt cgcgaactgc tgacagcgcg
     7081 catacagcgt atccatgccg gacgctttcg gcgacgactg aatgatcccg gcgttcgggt
     7141 tggcttccat caggcgcacc aggccgcaca aacaatcacc ggtcattacc gagtcagcat
     7201 ccagcaccac catgtagctg tactggctgc cccagcgacg gcagaagtca tcgatattac
     7261 cgcttttacg cttcacgcga cggcggcggc ggcgatagaa aatctgacct tcgccaccga
     7321 cttcagcgat aagctccatc caggcttttt gctctgcgac gcagatatcc gggttataac
     7381 tgtcactaag aatgtagaca tcaaaatgtt tggcattccc ggtggctttt actgattccc
     7441 acgttgcacg caagccagca aaaacacggt tcacgtcttc gttacagata ggcatgatca
     7501 acgccgtgcg atgctccggg tttaatggtt catcgccaac tgttgacgca gatatactgt
     7561 atttatcgcg accaataagc agttgcagga agcccatcaa cgccgtccag aatccggcgg
     7621 acacccaaca gaacagtacc gcaaagagga tcaggatacc ggtttgcagc atataaggca
     7681 gaagctgcat aaaggaaacc cacacatcct gaccaaccat atccatagga ttaatcagcg
     7741 cccacccctg ataaggaaga atggtcttca tataccaggt cgcgacaaca gtttgcgcga
     7801 gcgtcaggat caacagaatg taacggcgga tggtaccgac ggtacgccac ttttgctcac
     7861 tctcctgctc ttctttggtc aaacgagcca gatagcgcgg cgtcacatcg cgtccacgca
     7921 ggcgatccca gaaacggcct accgggttgg tacgccacgg gtcgggaaac atcgaggagc
     7981 gttttacttc tggcatcgcc tttagctgat cgcgcccttc gtcgtcttta attaactgtc
     8041 catcagcaag tgaatctggc caggcttgtt ccagacgcgc ctttaccgag ccttgcgggg
     8101 agtcatcctc ccgcgcccag gtgcggtgtt cggcatccag cgcctgatga acggcgcgga
     8161 tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat gggcattgcg tcaatgtact
     8221 cagttgtctt attcattggc aggtaactgg tagctccagg tttcactcaa cgtctgatcg
     8281 gcattcacca gcgcagcacg catttcagtg gttttcttgg catctttcac tttcacacgc
     8341 atcaccagac gccagccttt ggtaaccggg ttataacgca ccgtgctttc aactatctca
     8401 ccattatcac caatgctggt ttgcgctgtg accggggtat cctctggcag ttttttcatc
     8461 tcagcgccgg taaaatcgac cacaaaggcg atagtaccgt caggctggcg aatcaggttc
     8521 gactgcttca catcccccgt tgaacgacgc gtttgttgca cccatgcgtt atctggcgca
     8581 tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt taaagttcat ctctttaccc
     8641 ggctccggca gctgatccgg cgtccagtaa gcgacgatgt tatcgttggt ttcatcgttg
     8701 gttggaattt ccaccagctc aacgctgcct ttgccccact cccctttcgg agtcacccat
     8761 gcgcttggac gaagatcgta acgatcatcg agatcttcaa agcgggagaa atcacgacca
     8821 cgctgcaata gaccgaagcc ttgcgggttt tccatcgaga agctgctgac cgctaaatgt
     8881 ttcgggttat tcaacggacg ccagatccat tcgccattac cagcatggat agacagaccg
     8941 ttagagtcgt gcaactccgg acgatagtta tttgcaggcg acggttggtt cggcccaaac
     9001 aggaacatac tggttaacgg tgcaaccccc agtttgccga ctttatcgcg cagatagatt
     9061 ttcgactgca catccacaac cgtgtcacgt cctggcataa ctacgaattt gtaagcacct
     9121 gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac gtttatcagt cggttttgga
     9181 cgctcgatcc agaactcttt gaagcgtgga aattcttcac ccgatggcaa ggcggtatca
     9241 attgccaggc cgcgtgcaga aaggccataa acctgacctg caccaatcac gcggaaatag
     9301 ctggccccga gcatgctgac gatttcatcg tttttatctt tgctgttgat cgggtaaagc
     9361 actttgaaac cggcaaaacc aaggtctttt accgtgtctt tgtcatgctg aacatcgccg
     9421 aaagtgaaat aatccgggct gtatttgatt cgtttgactg cggtggcagt cacttcattt
     9481 attttgaccg gggtatcgaa gtacataccc tgatggtaga actcgagttt aaatggggtc
     9541 ttcagattgt tccagtacgc tttgtcatga ttaaactgga tctgctgata gtccgcgtat
     9601 ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg cctcatagcc tttcccggct
     9661 aaggattgag cttgctttgc gacatcatca atactgaaag cccagcttga agatgtatac
     9721 agggttaaca ttactgcagc actcaaccaa cgcattttca tcatttgtag tttatgtttc
     9781 ataattagta agcacttccc cctttgtgtg cttatatcga tccgatctat tttaatggat
     9841 aacctgccat tccgacaaca gaatcccggt atcgttcagc acactggctc atggattaag
     9901 cacctgattg caaccgtttt cactttccgt gcttctccta aagacagaag catcgacttc
     9961 gtgtagggtt tgccagcaaa tttatttatc aaccgtctta cagacgcttc cattctggtt
    10021 agtggtagcg atgatgctat gcataaaaac cttgaagcct gcgcctggcg cggtcaggtc
    10081 ggtgtcgcta tggcaacaag cctaaaggca taacccgaca gggataagac gaaaaatccg
    10141 agattagtta accatatatg aatccagtac ccgcgcaacg tgaatatttc ctcgactcca
    10201 tccgcgcctg gctgatgttg ttagggatcc cttttcatat ttctttaatc tattcgagcc
    10261 atacatggca tgtgaatagc gccgaaccgt cattgtggct gacccttttt aatgacttca
    10321 tccactcgtt ccgcatgcag gtgtttttcg ttatatccgg gtacttttcc tacatgcttt
    10381 ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt cgaacgtgta gggatcccga
    10441 tgttaacagc catcccctta ctgacattgc cgcaatttat tatgctgcaa tacgtcaaag
    10501 ggaaagcgga aagttggcct aggctgtcat tgtatgacaa atataatacg ttggcctggg
    10561 aattaatatc acacctgtgg tttttactgg tgttagtggt catgacgacg ctgtgcgtat
    10621 ggatatttaa acgcatcaga aataatttag aaaattctga taaaacgaat aaaaaattct
    10681 cgatggtaaa attatcggtg atttttttgt gcctcggcat cggttatgcg gtaataagaa
    10741 gaacgatttt tattgtgtat ccacccattc tgagtaacgg catgttcaat tttattgtca
    10801 tgcaaacgtt attttatttg ccgttcttta tcctcggcgc actggctttc attttccctc
    10861 atcttaaagc cttgtttacc acgccgtctc gtggctgtac ccttgcagca gcattggcgt
    10921 ttgtcgctta tttactcaac cagcgctatg gcagtggcga tgcctggatg tatgaaaccg
    10981 agtcggtgat cactatggtc ctcggtctgt ggatggtgaa tgtggtcttc tcctttggcc
    11041 accgtttgct taacttccag tcagcgcggg tgacttattt tgttaacgca tcgctgttta
    11101 tctatctggt tcaccacccg ttaacgctgt ttttcggcgc atacattaca ccacacatca
    11161 cctccaactg gcttggtttt ctctgtggcc tgatattcgt agtagggatt gcgataattc
    11221 tgtatgaaat tcatttacgc atcccattat tgaagttttt gttctctggt aaaccggttg
    11281 ttaagcgtga gaacgataaa gcaccagccc gttaagtcgc gtttacaata accattccac
    11341 gggcaatatc gacgccagtt tgaccataac ccgcttccag aaactggtgg cgggttcttt
    11401 tttgagaata atctctttct ttgcatgacg atcaacccag ttgatccgtc cccacctgtc
    11461 cagacggagc tgccaggccg catcatactg gctctgaata aagcgtttat caattaactg
    11521 tgccagcgtt tcgctctcta tcacgaagcc catttcagta ttgagcaacg ttgaacgcgg
    11581 atcgaaattg aaagagccga taaacaccgt tttaccatcg atactaaagg ttttagcatg
    11641 caggctggcc cctgaattac cggttatgcc gcgatcgtgt aacgtactac tttgttcacg
    11701 cgtcggcttg agttcatata attccacccc atagcggagc aattttttgc gccagcgcgc
    11761 gtatcctgca tggacgacag caacatcgtt agcggcaaga gaattggtta ggatcgcaat
    11821 ctttacccct tttctcacca tccgtaagag ttgcgccaca cctgcacgtg tcggtacaaa
    11881 ataggaagag ataatatcga tgcgttcact gggtgagccc atgatatcga acaggcgctg
    11941 cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc gccggatcat cacttaataa
    12001 acgtgttttc gcccagataa gcgacaacgt tccatcaacc agatgatgta taaacggact
    12061 ggattccatt ttgcgtaaat aacgatgcgt catggcatcg tcatgccagg atgccggtaa
    12121 ctcgatgcga tccgccattt caccttccgg gacatccagc acctgctgta agggtgaaac
    12181 cgatttgcaa taccaatagc gggcgaaatc atcggcaacg tcctctacca cgggtcctat
    12241 tgccatgaca tctaaatccg aaaaaagtgg ctcttcccct gcaccaaaat aggcatcacc
    12301 aatatttcgt cctcccacca gcgtcaccac gccatcgaca gtgaaacttt tattgtgcat
    12361 acggcgattg agacgggaaa agtcggtgat ataaccaagc ggacgtagca agcgaaacga
    12421 gaaaggatta aaaagccgga cttcaatgcg tggatggctg tcaagcaagc gtaaaatgtt
    12481 atcaagtccg ggcgtattgt tgtcgtccag caacaaacgt acgcgaacgc cacgctttgc
    12541 tgcggctaac agggcggaaa acagtaaccg acccgacata tcgtcctgcc agatgtaata
    12601 ctgaacatcc agtgtatgct ctgccatttc agccagacga taccgggcgg caaacgcatc
    12661 cagacttttc tccagaggaa aaagtccaca ctgaccggga tgttgcgaac acagtggcag
    12721 caccgcgctc gccagctgag gcaaatcatt catctccttg ttgggtaagg agtctttcgt
    12781 agaggtgggc gttttcttca tcataacaga caaagtatac ctgttcgggt aaagcgtgac
    12841 gggtaataaa ttctgaaacg gtttttactg cgatttcagc cgctgccgca cgggggtaac
    12901 cataaacccc agtactgatt gcaggaaaag ccactgacgt atagctgttt gctgccacca
    12961 gtcgcaggct attgagatag gcatcctgca aaagctggtc ttcgttttgt tcaccaccac
    13021 gccagactgg cccgacggtg tgcactacgg ctttcgcggg aagatcgcct gcaagcgtaa
    13081 taacggcatg ccccgtaggg caatcgccct gctgctgcct gacttttaaa caggcatcca
    13141 gcagggccgg acccgctgcg cgatgaatgg ccccatcgac gccgccgcct cccattaatg
    13201 acggattagc cgcattcaca atcacatcaa cggccagttt ggtaatatca ccctgcacaa
    13261 catgaatacg cgttttcata acgcctcctt acaccttctt gtttcttaag tgtacagcag
    13321 gctttttggt taaatcgcat ttttctgctc agcaacgtga gttgttcaga aacttagtta
    13381 tattcgatgt tcattacagc cagctttttc tgtttatcaa gatactgcac ttttactgag
    13441 gctatttgcg cgtttttaac attgccgctc attaacgctt tgctggaata ggttttacgc
    13501 ggaacagaac cattctgtgg gcagttcatt tctacggctg atgactgggt gctgacgtca
    13561 catgccggtt ccacaatttg gccataaaaa tggatcacgc ccccttgcgc gctgcttcct
    13621 gcaatggcaa taaaaggaaa aaacaaactg ccgagcataa gagagtttaa aaaaggtctg
    13681 aacatttatt ctatttcctc aatgattaat catccttgag ggttgtgtta tccatacttt
    13741 caggttggct gattcgcagc agaccattct ctccaggttc atcttatgct cgatatttca
    13801 acaaattaag acttttctga agagggcggc cattgttgtg ataaatgaag tgactgtcca
    13861 tcagaaacag taacaactat tttcacccga tcgtccgggg gaatatttaa actcaacttc
    13921 gtcaaagcaa tgagttgatt agcaggcaat gaaagggtct tttcttgctt cgtctgactt
    13981 tgccctgaac tgccttcgcg cagggacaat atttgtactc tgcacagaca agattgagta
    14041 agagtgactt caggaataat ggtgtacata tccccttgct gggtcgtatt aaaggttatc
    14101 tgactggaaa gtgccgcaag gagtaataac gcattcatat tcttctcccg aaaaaaaaca
    14161 gggcttgcgc cctgtttctt tcatactgat gatgtattag tactgatgag cggtcgcgtt
    14221 gttaccaaag ccaacctgag tgacgttaac gctggagtta gatgcagtct ggtcaacagc
    14281 agcaccgtta ccgccaccga actgtttaac agtcatagta gaatctttac cattccactg
    14341 atcaagagta gcgctgttgc cgaaaccacg ctgagtcaga tcgattgagc tgtcatcaga
    14401 accttggccc acatctgcgc cattaccgcc gccgtgctgg gtaatggtca gatcagagtt
    14461 acgggcgtca gcttgcagag caagagcaga gttaccgcca ccgtactggt aaatattcag
    14521 ctctgaattc gggccgctgt tattaccgcc accaccgtgg ttgccaccgc cgccgccgta
    14581 ctgaggaaca acacctgcca gagcgctacc agagaatacg attgctgcaa ttgctgctac
    14641 ttttaaaagt ttcatgtaaa acccccatcg gattgattta aaagtcgaat ggaaattaac
    14701 gttgtgtcac gcgaatagcc atttgcgact gtctctgcac tacaattgcc gttttttgag
    14761 taccatactg tgtaatattt gctttattac cagaaccttt ctggataatc atcgcagtat
    14821 taccataagc accttgcgaa atactggcat cgttggcact gcccgcctga tcaatatatg
    14881 caaggttata atctcctgtc tgatcaatct ttgcccggtt gctactacct tcttgcgcaa
    14941 caaccgccaa aagttttgag cctccctgcc gtaactgagc actattatta gtcccagctt
    15001 gaccaattat ggctgcctga ttaaatgaag acttactcaa ttcatttacc gcaaagttat
    15061 attctgaatt agctaaatca taacctgctg cggctgcaat cccaggcgca cccagtattg
    15121 ttaacatcat aaataacaat ttgtttttca tgttgtcacc ctggacctgg tcgtacattt
    15181 aagaaattaa atcatttcaa cttggttgtt aacgcaacct gtattttgtt aacgctgcgt
    15241 tacgatggaa agtatgtctg cggaaatatt tttaataact cacccgcacg ttgtattttt
    15301 ctttttttgt tcataccaaa gtattaaaac ccacgtaaat acgctgttat ctacgcaaaa
    15361 atattttgtt ttcttttaaa tctccgtttt ccgctatcaa aaatcaccag acagtcattc
    15421 ttcttgcccg tcgctgattg ctgcgatatg tcttgcgcac aagccgtgac aaacctcgtc
    15481 tttttgcagt aaaaaattgt ccacggtggt atggagaaaa aacaagaacg tttttcatga
    15541 cgaaaggact acagcgaaat a
//
LOCUS       DAZFRE010000106        15504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFRE010000106 DAZFRE010000000
VERSION     DAZFRE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.106"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..402
                     /gene="istB"
                     /locus_tag="ACHKUI_004045"
     CDS             <1..402
                     /gene="istB"
                     /locus_tag="ACHKUI_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO6600136.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTEQRQGRYKTTLQRGVMAPRLLII
                     DEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRI
                     LHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(469..597)
                     /locus_tag="ACHKUI_004046"
                     /pseudo
     CDS             complement(469..597)
                     /locus_tag="ACHKUI_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(739..954)
                     /locus_tag="ACHKUI_004047"
     CDS             complement(739..954)
                     /locus_tag="ACHKUI_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600137.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            complement(1428..2030)
                     /locus_tag="ACHKUI_004048"
     CDS             complement(1428..2030)
                     /locus_tag="ACHKUI_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600138.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLIHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(2124..2330)
                     /locus_tag="ACHKUI_004049"
     CDS             complement(2124..2330)
                     /locus_tag="ACHKUI_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6600139.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(2526..3722)
                     /locus_tag="ACHKUI_004050"
     CDS             complement(2526..3722)
                     /locus_tag="ACHKUI_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFO6600140.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            3954..4187
                     /locus_tag="ACHKUI_004051"
     CDS             3954..4187
                     /locus_tag="ACHKUI_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600141.1"
                     /translation="MRKDASARTRDKEGIVFVRTGREAGVLIIAELFSEVVTVSQPVP
                     GEQETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            4519..4662
                     /locus_tag="ACHKUI_004052"
                     /pseudo
     CDS             4519..4662
                     /locus_tag="ACHKUI_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(4902..5102)
                     /locus_tag="ACHKUI_004053"
     CDS             complement(4902..5102)
                     /locus_tag="ACHKUI_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600142.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            5302..5871
                     /locus_tag="ACHKUI_004054"
     CDS             5302..5871
                     /locus_tag="ACHKUI_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6600143.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            6037..6438
                     /locus_tag="ACHKUI_004055"
     CDS             6037..6438
                     /locus_tag="ACHKUI_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6600144.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            6426..6836
                     /locus_tag="ACHKUI_004056"
     CDS             6426..6836
                     /locus_tag="ACHKUI_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO6600145.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            7120..8739
                     /locus_tag="ACHKUI_004057"
                     /pseudo
     CDS             7120..8739
                     /locus_tag="ACHKUI_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8819..9111
                     /locus_tag="ACHKUI_004058"
                     /pseudo
     CDS             8819..9111
                     /locus_tag="ACHKUI_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(9151..10185)
                     /locus_tag="ACHKUI_004059"
     CDS             complement(9151..10185)
                     /locus_tag="ACHKUI_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO6600146.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(10188..11153)
                     /locus_tag="ACHKUI_004060"
     CDS             complement(10188..11153)
                     /locus_tag="ACHKUI_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6600147.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(11207..11965)
                     /locus_tag="ACHKUI_004061"
     CDS             complement(11207..11965)
                     /locus_tag="ACHKUI_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO6600148.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(11979..13193)
                     /locus_tag="ACHKUI_004062"
     CDS             complement(11979..13193)
                     /locus_tag="ACHKUI_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO6600149.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(13389..13508)
                     /locus_tag="ACHKUI_004063"
                     /pseudo
     CDS             complement(13389..13508)
                     /locus_tag="ACHKUI_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            13569..14702
                     /locus_tag="ACHKUI_004064"
     CDS             13569..14702
                     /locus_tag="ACHKUI_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO6600150.1"
                     /translation="MHSENIAAYVGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KHYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(14956..15174)
                     /locus_tag="ACHKUI_004065"
                     /pseudo
     CDS             complement(14956..15174)
                     /locus_tag="ACHKUI_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4008 a         3655 c         3939 g         3902 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatct
       61 acggaacaac gtcagggccg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaat aggctatctg ccgttcagtc aggaagaagc aaaactgttc
      181 ttccaggtca ttgctaaacg ttacgaaaag agcgcaatga tcctgacatc caatctgccg
      241 ttcgggcagt gggatcaaac gttcgccggt gatgcagcac tgacctcagc gatgctggac
      301 cgtatcttac accactcaca tgtcgttcaa atcaaaggag aaagctatcg actcagacag
      361 aaacgaaaag ccggggttat agcagaagct aatcctgagt aaaacggtgg atcaatattg
      421 ggccgttggt ggagatataa gtggatcact tttcatccgt cgttgacaca ttgccttaat
      481 ctccgcaaag ataacctcgt cattccggac attctctgcc aggcatatgt cgatgtaatc
      541 cataaacgat ttcagcttaa cccttttgtg gccggtcttt ctggctggcg gttcagggga
      601 tgttttctgg ttatcgtgta ccacaaaact gggatattgc cccgatagtc acatttcaga
      661 ttgatcgcgg tctgaagatg tggcactgag gtaacgtgct ggtgtcactg gtattgctga
      721 agttatggtt tttcggggtc aggtgagcct gttgtgtgta tgctttttgt tcagatcaga
      781 ggggggctgt gcggacagcg gcttttgtct ccgggcattc acgcctgaac gatgaatgcg
      841 attttcagca atatttgtct ttgtgacact ctctggttga gaagctaatg cgcgcccctg
      901 tttccacaga gaatgaggct tgccatcagc cgaaacatca gtattccacg ccatacagac
      961 ggcgcctgac catgtgctga tgcaggtaaa atgtttcaac tacaactgtt cgggttgcta
     1021 tgcaacgaaa cagcgattct cacgatcttt aactgcaacg tttttccgcc tgaaagctga
     1081 ctgcctcctg ctgaggtggg aggctatgat tcagtgcgtt tatagtctcc ggggtatgca
     1141 tgtaccggag cataccattt ctggtggata acaagccggc acattcagcc tgcagtgaag
     1201 atggaggcac ctggttgtga agaacgtttc tgcccgtggc tgcttcgcat catcaatggt
     1261 attcataccc gctcagaaat gaccgggtgc tgaagccctc cgttatcgcg cagtataatg
     1321 acgtatatga tctgtggacg agtgagcggc gtgcctgatg tacgccgcta ttactgtatt
     1381 ttaccacagg gctggttttc tgctgccagc cctgtggtta tcagacatca actgtgagcg
     1441 gcatcaaccg atgtacggta tgccataagc acgtactgat ccatcagatt tgtcagtggt
     1501 tcactctgac cctgacgtgc aatgatccat tgctccagat ctttttctga tttgtgtttc
     1561 aggtttttcg tccagtcgtc aaggctgttt ccggtcatta agtcaagcca gcagagccag
     1621 accagtttga gacgtaactc cgtgccgcca aactgacgga agaggctgat cagtttgttt
     1681 gccagagaat gttgcttcag gcgctcacat tcagacagca ggatcccttc cagcatcgga
     1741 tgatgaatca gcaggtgttc gctggtgcat aaattcacca ccagatcatc atccagggac
     1801 ggggagctcc gtttgtccgg accataaatc agccggtgca gactctgggg aacgatggca
     1861 atctccggtt tttcgccatt gaatgacacc cacccgggcg tcagattatg gaaatagtag
     1921 atattcggat gacacacgcc ccatgatacg gcgtgccggg tgaagccggt cagccatttc
     1981 tggtagtttt catggagcag ctcgtaacgg gtctgatact gttgtttcat tttcatattc
     2041 ctcacggggg ataacaggca gcagagcgcc gccggcaggc cggagattgc gtctgctttg
     2101 ggcgaagggt tagtggttaa aaattacgga cgggactgtg caatacgcgc ctgcagccag
     2161 gcttccactt cacttttcag ccagcgggag ctgcgcccca ttttgatggg ggcaggaaag
     2221 cccccaactt tgatgagttt gtaaaaccac ttatcggtta agccggtcag ttgagtgata
     2281 aacgccatgt cgaccatctg gtcatccatc agcgaaactg gggtagccat gagaatgttc
     2341 ctccggatgt tgttaaacca gccggtgtgg tcatgaaaac agcgcgccac cttcaccggc
     2401 ataacaaccg gggagaaacg taaaaaataa tcgggaatct ggctgtccgg agataaacgg
     2461 ggggattgcg atggcagagc cgtagcggaa tcccgggggg tgcccctatt aagttgtcaa
     2521 gcatgttatg acgcctgcgg ggtataaaaa gtcccgtcgc gcatcatggc gaacagaacg
     2581 tcgcagcgtc gtctcgccag ggcgataagc gcctgattgt gtcgttttcc ctgactcatt
     2641 ttgcgggtgt agtaagccct ggagagcgga tccctgagcg cggcgaaggc cgacaggaac
     2701 aacgcccgtt tgagagcttt attaccccgt cgcgagggat gctcaccgcg tattgacgag
     2761 ccggatcgcc gagttaccgg cgcaaggcca gcataagcag cgagatgtgc gacagaggcg
     2821 aaggcgcggc aggcgacctc ggtgaggagt ctggctgcgg tcctgacacc gactccgggc
     2881 atactggtca ggaccgggta aagagggtga gcaagaactc gctgttctac cgcaagcgcc
     2941 acctcgtctc tttgcttacg cagcgtgatg agctggagtg ccagacgtgg cagtactacg
     3001 gcagcggcat tcgtgccggg aacgacgacg gtttgttcgg ccagtgcctg agctatgtct
     3061 gctgcaaggc gtttacccag acgaggcgca agtttgcaga gctgggctgc cagcttcttc
     3121 tcacccagcg aagcgagttt ttctggtgag ggatatcgct ggagaagatc gagtaccgcc
     3181 gggtgatcaa gtctcggacc gagaacgcgc tccagtgccg gatgtatctg ggtcagaagg
     3241 ccgcggatac ggttgctggc ctgcgttgtc tgtgcggcaa gatcatcatc gaagccgcag
     3301 agcatggaga gttcggcgat ttgctcgtca gccagtttca gcgtgcgtag cgcgtgaggc
     3361 agggtacggg cagcttcggc aatgatggca gcgtcacgag catcagtttt agcttcaccg
     3421 gcgtgtaagt cggctatgcg gcgcatggcc agtccaggga ggtatccgac aaggactcct
     3481 tctgagcggg caacggcgac aggtaacgca ccgatggtag ctggctgatc aacaaccagc
     3541 agtatctgac catgttgttt caggtcagat attagcgacc tgagtttgtt ttcgtcgttg
     3601 ggtaatgctt tatcgaacag gcgtttacct gaacgattaa tggctacagc gtgatgcgta
     3661 tctttaccga catcaacgcc gataaagacc tggacggatt cgtaatcgct ggattcggtc
     3721 attctgtctc ccttgtatat gggttaacca gataaccacg gggagcaggt accggcatcc
     3781 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
     3841 cccaccagac ccggtgacat caccccccgg atcatggacg actgggggca gtaatcatgc
     3901 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg ggggtgcgaa
     3961 aagatgcttc cgcacgcacg cgcgataaag aggggatagt tttcgtgagg acagggagag
     4021 aagcaggcgt tctgataatt gcagagttgt tttcggaggt ggttacagtg agtcagcccg
     4081 ttcctggcga gcaggagact gatatcaccg atgggataag gttgtctgat tggtattata
     4141 aatataagaa gtcatcagag agtaacgtaa aggatttgcc agaataacct aagagagtaa
     4201 atctctccgg taacagagcg ctgtgaagca ttctgtatca gagtgcaaat acatgaacgc
     4261 tgatgcgtaa aatcagcagt gacacgccag ctccccctat gcccattcct gtataagaca
     4321 tgaactgagc gtattaatgc tcctgccctg agactgagcc ggtgagtgag gggctgagaa
     4381 atccctcttg acactgctct aaccagaaat acttccccgg ttgttgcggt gcgtccggat
     4441 ccccgataac ggaacactaa ggctgtattg cgggacacca ggtaagtggc agcccgtaat
     4501 acgtcaggag acgcgggaaa agtgaattcc cacacgcgtt ataaacttac ccttgcggag
     4561 ctggaagcct ttaactctgc cgttgacaac cggctggcag aactgaccgc aaattttacg
     4621 agtgcgtgct ggcttcagtc ttgtcacctg agcgttcggt aacggaggcg cgggcggtaa
     4681 tagactgatt ttcaggtatt accggcttac ggcgtgtggt tgatattgag gggctgtgag
     4741 tttcgcgtat ttgtcagaaa cccagacgag ttttcctgta tgggacgaaa taaggcagcg
     4801 gcaatacctg gaaaaattta atatggtatg aaggagaacc tgtcggttac gcttaatata
     4861 cccggtacac tgtcagctca ggggatacat gaaccgttat ctcaccagct acacagtata
     4921 tacacactga cgctgacagt agtcagcagg accacaataa tcagtgccag cagtatggta
     4981 acgatacggt ctgtattccg gtaatagccg aaggggtcga cagaaccaca atacgggcaa
     5041 cacactgtat tatgagtaat cgcgtttcca cagtccctgc acttcttttt gcgtatcacc
     5101 atcactccct cctcacagcc ttacccgtaa ccggtttttt acattaaaaa aacaacgctg
     5161 aaaattcaac cgtcagtccg gagacgaccg ttcgggttat cacagagtgc ctgagacagt
     5221 gccctgccgg aggtcacttc agggactctg cgcatttttt acgacgtggc tattccgtat
     5281 gaaccatacg gagatttaac catgacctac aaatacaacc ccttctggca gcaacgtatt
     5341 cgtgagacag tgcggcacgc actgaatgtt catccccgcc tgacggcatt gcgggttgac
     5401 ctgcgtttcc cggatgtacc ggcagcaacg gacgcagctg tgatatcccg cttcatcaat
     5461 gccctgaaag cccgaatcga cgcttaccag aaacgtaagc atcgggaagg taaacgcgtg
     5521 catcccacca ctctgcatta cgtctgggcc cgggagtttg gggagtgcaa aggtaaaaaa
     5581 cactatcatc tgatgctgct ggtcaaccgg gatacctggt gtcgtgccgg tgattaccgc
     5641 gctccgggat cactggccgg aatgattgaa caggcctggt gcagtgccct gggggtggat
     5701 gccggatgcc atgccacgct ggtgcatttt ccggcctggc cggcggtgtg gctggcgcgt
     5761 aatgatgaca ccggctttca gcaagtgctg gaacgtgctg actatctggc gaaggagcat
     5821 accaaagctc actgcaccgg tgagcgcaac tttggctgca gtcgtggctg agccagacag
     5881 acgagttatc tgacttcagt gtgatgacgt gttcatggcc accacactga ttttccctta
     5941 tgagactgaa ctgaaaccgc cattcacacc tgaccacgct gcccgtaccg ggacggctgt
     6001 tgcctgcgtg tcgtctgacg gtaaggaaca tatactatgt acgcaaaatc ctttctcgct
     6061 cttgatagca acggacgtct gacgggcgcc cgtactgtac aggccgcacc ttatgctcac
     6121 tacacctgcc acttgtgtgg cagtgcactc agataccatc cgcaatacga cactgaactt
     6181 ccctggtttg aacacactga cgacaggctg acagagcacg gtcaacagtg cccttatgtc
     6241 aggccggagc gcagagaaat acagttgatt aaacgtctgc agcaattcgt accggatgcc
     6301 ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat gtcaccacga ttattatggg
     6361 gagcggtact gcacacactg ccagaccgga ggattcagta ttccccggac aactcaggag
     6421 gaaatatgcg aattctgaac tgttatatgg cgaatgacag caaaggccat tttgttacgg
     6481 cgaaagaagc agcgaagcac aaccgccagg acgttttgtg ctgtgtgtcc tgtggatgcc
     6541 cgttaacact tcagcggggc aatgacggac aaccaccgtg gtttgaacat gaccagatga
     6601 ctgtcgctga gaaaatcctg ctgcgatgca cctggcttga cccggcagag aaagaggccc
     6661 gtcgtttgca tctgcagggt atgacggttc cggattatac ggtgaaggtg agaaagtggt
     6721 tttgtgtgat gtgtgacgaa gattatgaag gagaaaagtg ctgcccgcgt tgtggtaccg
     6781 gggtatacag cagggagggg gggctgcagg aaggcaactg gaaggatagg aactgagcgg
     6841 ttcagtcgcg gaaatcagtt gtagagctgt aatgatatct ccgggagtgc tgcgttggta
     6901 ggggcatccc gggatagaga aattttagca ctttgccctg aatgattgta gacgtcccgg
     6961 gtactcgtac cggtctgagt tattacagtt agatattcac tttttctccc gcatacttca
     7021 catctgtcaa cgacggatga aaagtggttc ttccgcaacc tgggtggaag aaaatggatg
     7081 aaactttctg gcgaacaagg atctaagctg ttttaagtta tgggcaacgc aatgcactct
     7141 cttaagacac ttctacagtt accttgcgga tggcgatgca gtcgacaaat tattagctct
     7201 gacggtataa ccctccatct ccacggaaaa cgcaaaacag cacaatgtcc tgaatgcttt
     7261 aagcgtagcg actctgttca tagttgtcgt cggcgccgga tacagcatct accctgctca
     7321 gggcagacgc tatggcttgt attttccgtc cgccactggt actgccgtaa cccggcttgt
     7381 taacgtaaaa tttttgccga gtcgcttgct cccttcgccg gttcacacca gcagtcttca
     7441 caggcgttac aaaatttaca acgtcaactg ggattaatag ctggaggtga ggctggaaaa
     7501 cgggctgcaa cggcagcggg tctccgttgc agtgctgata ctcttcttcg cagggttatc
     7561 aataccccgg agacgaaaca gtcaggcgcg cctcatgtcg gtattgatga gtgggcgtgg
     7621 catcggggcc actgctgcgg tatgttaata gtcaatcttg atactcaccg tcccctcgtc
     7681 ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt tcagaaaata tccggaaata
     7741 caggttgtct cgcgtgatcg cagtggagtc tatgcgacag cagcacgtga aggtgcacct
     7801 caggccagac aggtggccga tcgatggcac ctgctaaaaa gtattggtga tgagcctgaa
     7861 cgaatgatgt acagacatat gcctctgata cgtcttgttg tcagagagtt atcactgaat
     7921 aaatcacctg agccagaaat atctgtgcct gtagcatcgc tccgtcgtcc ggaacgcctt
     7981 aaacagcaaa cccgcaaaaa acggcatcag cattggacag aggttatggc cctgcataac
     8041 aagggatgta gtttcaggga aatatcccgt attacaggcc tgtcgcgagt gacagtcagt
     8101 cgctgggtgc gttcaggaac attccctgaa atgtcaaccc ggcctccaaa gcgagggctt
     8161 ctggacccat ggagggagtg gttaaaagag caacgagaaa gtggtaatta taatgccagc
     8221 cggatatggc gggaaatggt ggcccagggg gggacaggca gtgaaaccat cgtcagggat
     8281 actgttgcca aatggcgtaa aggctggaac ccaccggtta ctactgccgc aagacttcct
     8341 tcagtgtccc gggtaagccg ctggttgatg ccctggagaa taatcagggg cgaagaaaat
     8401 tatgcatccc gatttattag tctgatgtgt gaaaaagaac cggagttgaa aatagcacag
     8461 caactggtac tcgagttcta ccgtattctg aaaacctaaa ataaatcaca gctaagcagc
     8521 tggttcactc gagttcacga aagcggctca gcagaacttc ggtgcgtagc tgcggggatg
     8581 gaagctgatg ctgcggctat atgtgaggca atcagcagtc gctggagtaa tggtgttgtc
     8641 gaaggtcatg taaatcgcct gaaggtgctg atacgccaga tgtatggtcg agccggattt
     8701 gaactactta ggcggagggt aatgagtcca ctggcatgaa aatcttcacc taggttgcgg
     8761 aagagccact tttgcactgt tcgtgacagt atggccttta tgtactcact ttgttaccgg
     8821 tagctgaatc atcactaccg tgctgcaaat ggtggtgaac agcatcaatt gtggaatgaa
     8881 tgttgccgaa gcgaccaatg cgcctcgttt ccaccatcag tggttgccgg actagctgcg
     8941 tgttgaaaag ggtttagtcc ggatacgctc aagctgctgg aagcaaaagg tcagaaagtg
     9001 gcgctgaatg aggcgatggg cagcacgcaa agcattatgg ttgggtcgga tggtgagttg
     9061 tacggcgcat ccgactcgcg cttggtggat gatttaacgg cggggtatta attgacatcc
     9121 cccggcgcag tgtgccgggg gataatggtt ttatgcggtc aataatcgtc cgggattatc
     9181 gatacagagt tttttgagga tcgttttatc gacgccgcgc tccgccagat aggccagaaa
     9241 aacatctgga acaaatcccc agccattacc gccattttta gcccacattt gtttaaggaa
     9301 gacatcgtgg ctgagaacaa gctgatcggc gtatcccaac tcaatgagat gagcgacggc
     9361 atcggcagtc tcctgcaccc ctggcgctat tccctctttc gggaaggtaa tgtctaggcc
     9421 aatcatgtcg aattccagcc agacaccctt atcaagcatt ttccgctgat acgccacgtc
     9481 ttttcctgac ggatccgagt gtgcgagaga gactttattt ggcgagacgc ccatttcacc
     9541 taacacaatg tcgagtactt catcaccgcg acgaagccag cccggcatgt gaatattcat
     9601 cgccacatga ggattgttaa tctgtgccag cgaggcagcc cgcaagctgt tatgctcggc
     9661 ttcggtaaat gtcggtgaga cacctatttc accgatcatt ccggcacgaa tatccgtatc
     9721 gccaatccct tgattcaatt ctttatcgat cgttgccgcc agttcatcaa ccgttttatg
     9781 aattctctgg ctttcaaatt tttccaggta gggaccggag gaagcaacaa tattcagacc
     9841 tgtttttaat gcgacttctc gtaatgcctg cgcatctcgt ccgatagact cagaccctgt
     9901 ggcatcaaca atggtgcgac caccgagcga gataaagtta ttaatttcga agatcacatc
     9961 ttcaataggt ttgcggtcca tattatcagc gcagcaataa ggatcgtgtt ttaatgccca
    10021 ctggatttct gccgtgactt ttttatcaac aagccgttgt gaaaaaggat agcaaggagc
    10081 atccacaaca gaggaaagat cgttgaacag atgttcatga ggaagtgtta gccccatatc
    10141 ttcacgggcg acggggcccg tcacagtttg tagataatct ttcatgatta tacctccatt
    10201 ttcttcattg ttttcgaggt tctgtattcc ggaatagtgg taacaattgc accgaccagt
    10261 gcaaacagtg ttccgatgat cgtgaccaga taaacggtat tgcccagcga tggaattaat
    10321 acgtcaatca atactgatcc cagtaactgg cccgccgttg aagcaacccc cagcatcagt
    10381 aaccccagac ctctgaccaa aatggccatt aaaccgatag acattagtcc aagcgggccg
    10441 ccaagataca tccaccagac atctggtaat tgaacagtga cgtgccctaa tgcgacacga
    10501 atagctagcg ctgtgcctaa aacacaaaat ccaacgatga aattccaggt gattgaaacc
    10561 agcatggatc ccgtagcttc agctactttt gcatttccag cgggctgcca tcctgcaagc
    10621 aaaccagcta agaacggaag aatggcaagt aagatgaaag aggtagaatg ccactgcggt
    10681 gacacaacaa aaatcgtagc aataaccgca aataatgcac cgatgatgcg ataaggggta
    10741 aagtattttt tctcgtcgac accgatacca aagcgatcgc aaagtaaacc agacagcaat
    10801 agcgcggaga tcagtgcggt ttgaaacgtt gcgaccccga gtgcgctggc tgatgcgcct
    10861 tcagaaaata ccaccatcgc tccacagaga ccggcaaacc agttccagag aggaactttt
    10921 cggtttttaa tcagagaagg aattgccgca aattgttgcc gggtttcttt tcgcgccata
    10981 ataataaaaa acatcacgac taaaccactg gcaaaggaga taactgcgca tgcattacca
    11041 tcctgtaacc agtgacctaa ttgaccattc accgctgatt gcatagggga gagcataccc
    11101 gcaagaatcg tggcaagcat ccataaggga gttgagtact tattcttttt catacatcaa
    11161 tccttctatt atagtaaatg gggtaactgg agttacccca ttattattat cttgcaatat
    11221 gagtcagtat atttacccac aacgcggtat agaacggcca ggacataaac tgctgtgtac
    11281 cccagtgcgg tgaacagtca ctcataaagc aggctatttt cccgttgtga tagttaccaa
    11341 agaccagtaa tggatcgttg tttattgtta ataccacctc agcattttct ttagcgatcg
    11401 cccggttata acctaggaaa aaaggatatt cagagaaccc tttaataact ggatgttcag
    11461 gttgtgatgg ctgtgcaata acaccctctg gtttttcgac acgatcgtca ccatctaaca
    11521 tcgtaaccgg gagcacatca gccagaactg tatttttata attggctttt gcttcaattc
    11581 ccatgaatga gagatagccg ccaatcatca ataacccacc accgttattc acatattctt
    11641 taatcagttc gagagcgttt ggttttatgc gaagttgata aaaagtatca ttttgtagca
    11701 ggaaagtatt actaccaata tcactaataa cgatggcatc atattgttct aactgtgcca
    11761 catcttcagg gaaggcaatt tgtaccgtat gtgctggcat ataggtgaca tctacctgac
    11821 tatttttcag acattggagt aaccaggtcg caccttcttc atatttactt gaggtaaaac
    11881 tgtcatatcc ttttgaatga atcatatgaa tatgccagga ttccccaata aagagcactt
    11941 ttagtgattt tttggtgtta ttcattctct gtcccttctt aatgcgtaaa aatagtttgc
    12001 tgataatgta tactctgaat tcgatgtatc acagattcat gttcaggcat gtcggaagca
    12061 ttgcttgtct ccaccgctag ggatgcaaag gcgctggcat aacacagtgc tgattccagt
    12121 gattttcctt tcgcaagtcc tgatgccaga gcgccgttaa aagcatcccc ggctcctgcc
    12181 gtatttttaa cgaccgctgg aaacgctggc gaatgaatga attttttgcc atcaaatgcg
    12241 agcgagccct tgctaccgag cgtgataacc gtgtttttta cccctttatt gtggatagct
    12301 tctgcagctc gtttagctga ttcaagatcg tgtacgtcta tgccggacag taaaccagct
    12361 tctgtttcat tgggggtgat ataatcaaca tcctgaataa gttcgttgac gatatcatta
    12421 tagggtgccg ggttaataat gaccgggata ctgttttttt gcgccaatgt gatcgcttgt
    12481 tgcagagcgg tataatttgt ttcgagctgc agaagtatga catcagaatt aataatcgct
    12541 tctttctgaa tagttatttc atcggacgac attgtcatgt tggcaccggg atagatcgca
    12601 ataacattat cgccagtacc ttcattaaca aggatcgtgg cagttccggt ttgcgtttct
    12661 ttcgtttgat aaattatact tttataaatc ttagacgaat tcataaagtt gatcgcataa
    12721 tcactaaagt gatctgtacc tacttttgtt atgaaataaa catcagtatc ggcaaagctt
    12781 gctgccagcg cttggttaca gccttttccc cccggtgaaa atataaaatt acttgccaga
    12841 agcgattctc cgatagtggg aagcctcggc aaataactga tgatatcaac attaaacgaa
    12901 ccaataacgc atacttgact tttcatagtt gagtggcctt tttttattcc ctgggctgta
    12961 tcataatttt caagcgcata agcgatttca tttttcgcaa tagtatcaag cgagtctagg
    13021 acgataaaag caccgccatg acatcgagtc agatagccaa gcctggcaag ttcattaaga
    13081 tctgatcgaa tagtttcttt cgttacattg aggctattgg acagatcgga tacttttaca
    13141 gaaccgaatt tactgagtcg tttcaatatt tcatgatggc gttcaaattt catgcttcac
    13201 ctcatttcag ttacgttaat atctatcgat gtatattgat gtgataatca ccataaagtg
    13261 gcggggaaaa gcaaaaaaaa gcaaaaatag cctgagaaaa ggtaagcgtt aatggagaat
    13321 catattgatg cttatttatt ggtgagaact acgttccatg gcaggagttc atcaatacgg
    13381 ttggaaggtt attccggcag tacgctcagg atatggcgca gatacgcttc cggatcaatg
    13441 tcgttcagaa ggcaagtgcc gataagccca tacaacagtg cactgcgctc gccgccgaag
    13501 aacataaaga tgatatgacc tttatatact cactatagca ttggtgggat attccagagg
    13561 aggtcatcat gcattcggaa aatattgcgg cttacgtcgg gctggatgtg cataaagaaa
    13621 cgttggcggt tgctattgct gcacctgaac ggcttggcga agttcgttat tatggtacga
    13681 ttaacaacga agcccaggct gtacgtcgat tatttcagaa attgcaggga ctttacggaa
    13741 acattctttc ctgctatgag gctggcccct gtggttttgg gctatatcat caactcacag
    13801 cgatgaatat aaaatgccag gttattgccc cttcccgcat accaaaatca ccgacagaca
    13861 gaatcaaaaa tgatcatcgg gacgcaattt ctctcgccag gttactgcgg gctggagaac
    13921 ttacccctgt ctggatccct gacctgactc atgaagctat gcgggatcta atcagagccc
    13981 gtgcagcagc taaacgagac agcagagttg cccgacagcg aattctgagc atgctattgc
    14041 gaacagacaa acattatgca ggaaagcact ggacaggaaa acatcgaacc tggttagcta
    14101 atcaatcatt tagtcagcca tctcaacaga ttgcttttca acactattgt caaagtctgg
    14161 agcagattga agatcgcata cttcaactcg accaggaaat atctcgcctg ttacctgagt
    14221 ggtctctgtg caatctggtc tgccagttgc aggctcttaa aggtgttggt cagctaattg
    14281 caataactct ggttgctgaa ctgggtgatt tttcgcgatt ttccaatccc aaacagctca
    14341 tggcttttct gggactggtg ccaggtgaat attccagtgg aaacagtatt cgtcccagag
    14401 gaataacaaa agttggaaac agcgaactga gacgtctgct ttacgaagcc gcctggtctt
    14461 atcgtacacc tgcaaaagtt ggggcatggc ttatatatta ccgaccggac tctgtaacac
    14521 aatattccaa agatattgca tggaaagctc aacaacgatt gtgttctcgt taccgaagtc
    14581 tgacagcaaa agggaaaaaa tcacaagtag ccattacggc ggtggctcgt gagttgactg
    14641 gatttatgtg ggatattgca cttgctgccc aatcatcatt cagtcagcag aagcaaaatt
    14701 aaaccctgaa gcagtcagac acggaatgag tgatcctcgt aaaagaattc agggaagcca
    14761 gaatgcgcta atacctgcac gtagaaagag gccagctcac tgcgtaaatc gtcatggggc
    14821 taaaatcccc gaataagagt ttgtaaaatc caagatacag tctgactgtt tcaggttctt
    14881 taatccgcga tataacgaag ctcccggagc tgcgtaaggt ctttttgggc ttaatatttg
    14941 acaaaggtca taagagtttt tatttccgag acagatggca caaagggctt tttccgcggc
    15001 attatgtctg cctccgcctg accgtcaaca ctgtaataac agagtgcgcc ccactgattc
    15061 agtacacagc tgaaccattc acccagtctg gatttttgga tagcgtggca tttttctcca
    15121 ccatgcatgc agtgagtcca gtaacagttt gctcttcttc tgtctgacca caagatgtgc
    15181 tggactggta aacgatgggg atacctgctt acgcgagagc aataagtggc tggccacaat
    15241 tctcggtaca aaactctgct gaatagcagc gattttccca gcgtggtgcg tcgtttgcat
    15301 tttactggtt ctaagttaac aattatctgt tcactaatga ctactctaaa aacttgtatc
    15361 aggactatca cttatttaag tgatattggt tgtctggaga ttcagggggc ctgtctagag
    15421 agatataaag ataccttcac cccggtgatg ataaaataaa agtatctcag ctattaaact
    15481 gatgtacgat tcgggcaata actt
//
LOCUS       DAZFRE010000107        14960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFRE010000107 DAZFRE010000000
VERSION     DAZFRE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.107"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(313..1962)
                     /gene="pgi"
                     /locus_tag="ACHKUI_004066"
     CDS             complement(313..1962)
                     /gene="pgi"
                     /locus_tag="ACHKUI_004066"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6600151.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2199..2395
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2487..3836
                     /gene="lysC"
                     /locus_tag="ACHKUI_004067"
     CDS             2487..3836
                     /gene="lysC"
                     /locus_tag="ACHKUI_004067"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6600152.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4089..4361
                     /locus_tag="ACHKUI_004068"
     CDS             4089..4361
                     /locus_tag="ACHKUI_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6600153.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4362..5234)
                     /gene="rluF"
                     /locus_tag="ACHKUI_004069"
     CDS             complement(4362..5234)
                     /gene="rluF"
                     /locus_tag="ACHKUI_004069"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6600154.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5582..6529
                     /locus_tag="ACHKUI_004070"
     CDS             5582..6529
                     /locus_tag="ACHKUI_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6600155.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDIQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6599..7405
                     /locus_tag="ACHKUI_004071"
     CDS             6599..7405
                     /locus_tag="ACHKUI_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6600156.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNSIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7415..7822
                     /locus_tag="ACHKUI_004072"
     CDS             7415..7822
                     /locus_tag="ACHKUI_004072"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO6600157.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7822..8316
                     /locus_tag="ACHKUI_004073"
     CDS             7822..8316
                     /locus_tag="ACHKUI_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6600158.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8382..9179
                     /locus_tag="ACHKUI_004074"
     CDS             8382..9179
                     /locus_tag="ACHKUI_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO6600159.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9190..10014
                     /locus_tag="ACHKUI_004075"
     CDS             9190..10014
                     /locus_tag="ACHKUI_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6600160.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10066..11295
                     /gene="sorE"
                     /locus_tag="ACHKUI_004076"
     CDS             10066..11295
                     /gene="sorE"
                     /locus_tag="ACHKUI_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO6600161.1"
                     /translation="MKTTALRLYGKRDLRLDTFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLIITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAVILAHHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11676..12365
                     /gene="pepE"
                     /locus_tag="ACHKUI_004077"
     CDS             11676..12365
                     /gene="pepE"
                     /locus_tag="ACHKUI_004077"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6600162.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12455..14086)
                     /locus_tag="ACHKUI_004078"
     CDS             complement(12455..14086)
                     /locus_tag="ACHKUI_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6600163.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14257..14706)
                     /locus_tag="ACHKUI_004079"
     CDS             complement(14257..14706)
                     /locus_tag="ACHKUI_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600164.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNDRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPLLLNYFYSQDLVLNISLFIFSEVTKVTTIIWCKRQGKV"
     gene            complement(14749..>14960)
                     /locus_tag="ACHKUI_004080"
     CDS             complement(14749..>14960)
                     /locus_tag="ACHKUI_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600165.1"
                     /translation="IKTGVEWVFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDV
                     LFSEGIADRGLKNTGGVLIQKRPQL"
BASE COUNT         3736 a         3733 c         3824 g         3667 t
ORIGIN      
        1 aaaataaaaa taaaagtaat taatctgcgt attttccgaa atcaatcacg attaaaaatc
       61 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
      121 gataaaaaat gccgacataa cgtcggcatt gtttttaagg ctatttaatc aatcgtacta
      181 tgtattaatt gccgaatacg atgtacatat cggcatcgac ctgtaggcct gataagacgc
      241 gaccgcgtcg catcaggcat cggttgccgg atgcggcgtg aacgccttat ccggcctaca
      301 tatcgacaat gattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct
      361 atcgtggctg ctgatttctt tgtcatcttt cagctctggc agaatacggt tcgccagctg
      421 tttacccagt tccacgcccc actggtcgaa ggtgaagatg ttcaggatca cgccctgagt
      481 aaagattttg tgctcataca gcgcaatcaa cgcacccagg ctgaacggag tgatttcacg
      541 cagcaggatg gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc
      601 aagcgttgcc ggatctttac cctgatcacg atattcctgc tcaaccactt cgcgggattt
      661 accaaacgcc agcgcttcgg tctgagcaaa gaagttagac agcagtttct ggtgatgatc
      721 agagagcggg ttatgggtga tagccggagc gatgaaatcg cacggtacca ttttggttcc
      781 ctggtggatc agctgataga acgcgtgctg accgttcgtg ccaggttcac cccagataat
      841 cgggccagtc tggtaatcca caaccttacc gttacggtca acatacttac cgttggattc
      901 catattgccc tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat
      961 cgcttcagtt tccgcgccaa agaaattgtt gtaccagatg ccaatcagcg ccagcagtac
     1021 aggcaggttt ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga
     1081 aagcagttca acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga
     1141 ccacaaagag taacgaccgc cgacccagtc ccagaattcg aacatgttgg cagtatcaat
     1201 accaaactcg ccaacggctt tggcgttggt tgagagcgcc gcaaagtgtt tcgcaacgtg
     1261 cttctcatca cctgccgctt tcaggaacca gtcacgcgcg ctgtgggcgt tggtcatggt
     1321 ttcctgagtg gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt
     1381 tttcagcact tccgcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg
     1441 gtttttgtat ggacgcagtg cttcagtcac catgtacggg ccgaggtcag aaccgccgat
     1501 cccgatgttc actacgtcag tgattgcctt gccggtatag cctttccact caccggaaat
     1561 aatcgcttcc gagaaggttt tcatcttctc cagcaccgcg ttgacttccg gcataacatc
     1621 tttgccatca accagaatcg gggtattgct acggttacgc agcgctacgt gcagtacggc
     1681 gcggttttca gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag
     1741 atcgcactct ttcgccagat cttgtaattt cgccagcgtc tcttcagtga tgcggttttt
     1801 ggagtaatcc accagcatct gatcgtcgaa ggttgcggag aacttagaaa aacgatcgcc
     1861 gtctttagca aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag
     1921 tgcctgccag gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat
     1981 tttgagaatt gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta
     2041 gtgccgttag cgtaatgttg agtgtaaacc cttagcgcag cgaagcattt attagctgaa
     2101 ctactgaccg ccaggagtgg atgaaaaatc cgcatgaccc catcgttgac aaccgccccg
     2161 ctcacccttt atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc
     2221 ccaagtaacg gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga
     2281 ggtgattgaa cggctggcta cgttcatcat cggctacagg ggctgaatcc cctgggttgt
     2341 caccagaagc gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata
     2401 gtagcgaagt atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa
     2461 aatggatccc ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta
     2521 ccagcgtagc tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca
     2581 acgtacgttt agttgtcctc tcggcttctg ctggtatcac taatctactg gtcgctttag
     2641 ctgaaggact ggaacctggc gagcgattcg aaaaactcga cgcaatccgc aatatccagt
     2701 ttgccattct ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc
     2761 tggagaatat tactgttctg gcagaagcgg cggcgctggc aacgtctccg gccctgacag
     2821 atgaactggt cagccacggc gagctgatgt cgaccctgct gtttgtcgag atcctgcgcg
     2881 aacgcgatgt tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgatcgat
     2941 ttggtcgtgc agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgcttc
     3001 cacgcctcaa tgaaggctta gtgatcaccc agggatttat cggcagcgaa aataaaggtc
     3061 gtacaacgac gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt
     3121 tacacgcatc tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac
     3181 gcgtggtttc cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg
     3241 caacttttgg tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata
     3301 tcccggtctt tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata
     3361 aaactgaaaa tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca
     3421 ctttgcacag cctgaatatg ctgcattctc gcggtttcct cgcggaagtt ttcggcatcc
     3481 tcgcgcggca taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa
     3541 cccttgatac caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga
     3601 tggagctgtc tgcactgtgc cgggtggaag tggaagaggg tctggcgctg gtcgcgttga
     3661 ttggcaatga cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac
     3721 cgttcaacat tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc
     3781 ccggcgaaga tgccgagcag gtggtgcaga agctgcattt taatttattt gagtaaacac
     3841 agtatggccc ggaagacatt atttcgggcc gtattgtttt tcttgtcatt atgcccataa
     3901 ataaacgagc ctgtactctg ttaaccagcg tctttatcgg agaataattg cctttaattt
     3961 tttatccgca tctctaacta attatcgaaa gagataaata gttaagagaa ggcaaaatga
     4021 atattatcag ttctgctcgc aaacgaattc ccgtacactg aactctctgg ctcactgagg
     4081 gcaccatcat ggcactcccc cgtattaccc aaaaagagat gactgaacgc gagcagcgtg
     4141 aacttaaaac actgctggat cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg
     4201 aaactaacag cattaagaaa gagtacatcg ataaattaat ggtcgagcgt gaggctgaag
     4261 cgaaaaaagc ccgccaattg aagaaaaagc aggcttataa accggatcct gaggcgtcat
     4321 ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg attaacgccc cttctttttg
     4381 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
     4441 ttttccatct taacgactgg acgtttgatg cctaccgttt tcggtttggc cttcgcttta
     4501 ggttttgcct cggaagagga attttcaatg agcttaaaga gatcgattaa ctcatcgtcg
     4561 gttaaatcgc gccactcacc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta
     4621 cgttccagct ttttcacttc gtaaccaaag tgctcgcaca tgcgacgaat ctgacggttc
     4681 agcccctgca ccagggtaat gcggaagaca aacggtgctt cttttttgac tttgcacttt
     4741 ttagttaccg tcccgaggat cggtactccc gcccccatac cacgaataaa ttcatcggta
     4801 atcggtttat caaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
     4861 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggaatc tttatccagg
     4921 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
     4981 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
     5041 tcggattccc gaggctcaat caactgacca tttactttca caacgtcgcc gggtttcacc
     5101 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
     5161 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
     5221 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
     5281 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
     5341 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taagaataat caacaaatta
     5401 cgcatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
     5461 gcgctatttc cccccatgca aagagtgaaa aatcgctttg aaaggccatc atgatggggt
     5521 tatatcagat caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc
     5581 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
     5641 ggatatgacg caggcgcaaa ttgcgcgcga actgggtatt tatcgcacta ccatcagccg
     5701 cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc gccatcaact atgactacaa
     5761 cgaaaatctc tggctggagc agcaagtgaa gcaaaagttt ggcctgaaag acgttgtggt
     5821 ggtgtcagga aatgatgagg atgaagatat tcaactggcg atgatggggt tacacggcgc
     5881 gcaactgctg gatcgcttgc tggaacctgg cgatattgtc ggtttttcct ggggccgcgc
     5941 ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatttgcgt
     6001 gccgattatt ggcggcccgt ccggtaaact cgaaagccgc tatcacgtaa acacattaac
     6061 ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggcgctgct
     6121 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
     6181 ctactgggat aatctggatg tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
     6241 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
     6301 tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat
     6361 gagcgaaaaa acactctcta tcgaaatgaa taaattaaag caggcacggt attccattgg
     6421 cattgccatg agcgaagaaa aatacagcgg aattgttggt gcactgcgtg gaaaatatat
     6481 taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
     6541 atgtttaccg taataaaaat tacggcgggt ccctactatc taaaatcagg agtctgttat
     6601 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggtggcg catccggtat
     6661 tggtctggcg attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat
     6721 tcacggtggc gatggtcaat atgaaagcca taaaggttat cagttctggc cgaccgatat
     6781 ttccagcgcc aaagagataa atcatacggt agcagaaatt atccagcgtt ttggtcgcat
     6841 cgacggtctg gttaacaacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
     6901 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
     6961 gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
     7021 cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggcc aaagctgtta
     7081 cgccgcgacc aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggcaa
     7141 gcacggtatc cgtgtggtcg gtatcgcgcc agggattctg gaaaaaacag gactgcgcac
     7201 gccggaatat gaagaagcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga
     7261 aggctatacc aaaaattcca ttcctattgg gcgcgccgga agattagcag aagtggctga
     7321 ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc accggagtaa ccactaacat
     7381 tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgcgca
     7441 tggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg atgccagggt
     7501 cgaggccgtt gaatttgtgc ccggcgagaa cgccggagac atcgtagcaa agctggaaaa
     7561 gttagtaagc attcacaatc aagatgagtg gctaatcgcc gtggatttac agtgcggcag
     7621 cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg
     7681 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atattgatga
     7741 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gtcgtctgga gacaggtggc
     7801 aacagcggag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgcctgatc
     7861 cacggccagg tcactaccgt ctggtctaaa gtggctaacg cccaacgcat tattatctgc
     7921 aatgacgaag tttataacga tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg
     7981 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa
     8041 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcaatggtt
     8101 cgccaggggg taaaaatagg taccctgaat attggcggta tggcctggcg tcctggtaag
     8161 aaacagttaa ccaaagcagt ttcattagat gacgatgata ttaacgcatt tcatgagtta
     8221 aataatcttg gcgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
     8281 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc
     8341 tttaacaggc agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac
     8401 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
     8461 ttcagactca tcgtccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
     8521 aaaccggaat tatgctcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg
     8581 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
     8641 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
     8701 aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
     8761 cagcggaaga agcacggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc
     8821 aggcactgcg cgttgctatt ccggcactga ttgtctcgct gttcgtcagc gctgatatgg
     8881 tgagcaatat gctgagcgcc attcccgaat ttgtgacccg tggactgcag attgctggcg
     8941 gttttatcgt ggtggtcggt tacgccatgg tgcttcgcat gatgggcgtg aaatatttga
     9001 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagt ctgctggcgt
     9061 tcggtggcgt cggcgtgatc atggccctgc tctacatcca gttaaatcca cagtggcgta
     9121 aagctgaacc acatccccag accaccacta tcaccgccct tgaccaactt gatgattaac
     9181 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
     9241 tccaacctgc aacaggcatc ctttaacttt gaacgtattc acgggctggg tttttgctac
     9301 gacatgatcc ccgccatcaa acgactttac ccattaaaag aggatcaggt tgcggcgctc
     9361 aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc
     9421 actgccgcca tggaagaggc gcgagccaac ggcgcggaaa ttgatgacgg taccattaac
     9481 ggcatcaaag taggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga
     9541 acgctgcgcc cgattaccgc cgcgctcggc gcatctctgg cactttcggg caacattctc
     9601 ggccccctgc tgttcttctt tattttcaac gcggtgcgtc tggcgatgaa gtggtatggc
     9661 ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg taatttgctg
     9721 caaaaactca ccgaaggcgc gtcgattctc gggctgtttg tgatgggcgt gctggtgacc
     9781 aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcctgccgc cgatggtgcc
     9841 accgtcacca taaccgtgca gaacatcctc gaccaacttt gccctggttt gctggcgctc
     9901 ggtctgacgc tgctgatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt
     9961 ttcgccctgt ttggtttagg gattatcggt aatgcgctgg gcttcctgtc ctgattcttc
    10021 gccccggcgc aaccgtcggg gccatcgttc aacacgaggt ggtttatgaa aacaacagct
    10081 ctgcgtcttt atggtaaacg tgatttacgc ctggatacct ttgaccttcc tgaaatgcag
    10141 gaggatgaaa tcctcgcaac ggtggtcact gacagcctgt gcctctcttc ctggaaagag
    10201 gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa ccccatcatc
    10261 atcggccacg aattttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc
    10321 cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggattgc
    10381 cccggctact ccttcccgtg ggtaggcggc gaagcgacgc atgtggttat tcccaacgag
    10441 gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga aggctcgctg
    10501 gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt
    10561 agttataacc acacgatggg gattcgcccg caagggcgca cgctgatcct cggcggcacc
    10621 ggaccaatgg gactgttggc gattgattat gcgctacatg gacccgttaa cccttcgctg
    10681 ctcatcatta ccgataccga caacgacaaa ttgagttatg cgcgcaagca ctatccatca
    10741 gaaccgcaaa cactgattca ttatctcaat gccgccgatg cagcatttga tacgttaatg
    10801 gcactgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg
    10861 gtgactctcg cctcttcctt gctggcgaca gatggttgtc tgaatttctt cgccggaccg
    10921 caggataagc acttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac
    10981 tacgtgggca catcaggcgg caataccgac gacatgcgcg cagcggtcaa attgattgaa
    11041 gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc
    11101 gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa
    11161 tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggt gattctggcg
    11221 catcatcagg ggatctggtc cggtgaggcg gagcaatacc tgctcgccca tgcagaggca
    11281 atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccgcagcaa
    11341 cttcgcggcc tgatgtgcgg ctgattgcag aagcggcaca atgtgcacga gaagaataaa
    11401 aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agtgcacatt gtttatgccg
    11461 gatacggaat gaagccttat tcgcctacat aatcttgaaa attcaatata ttgcagttat
    11521 ctggtaggcc aggtaagcgt agcgcatcag gcaatcctgt gtttgtcatc agtctcagcg
    11581 cctgtgcaat atgtgacatc gtgagccatg tcagtaagtg gttaaattcg cagtggtagc
    11641 attccttccg tgttttctta ctaaccggag aagtaatgga actgctttta ttgagtaact
    11701 cgacgctgcc gggtaaagcc tggctggaac acgcgctgcc gctgattgct gaacagttgc
    11761 agggtcgccg ttctgcggta tttattcctt tcgctggcgt aacgcagacc tgggatgatt
    11821 acacagcgaa aacggctgcg gttctcgctc cgctgggtgt ttctgtcacc ggtattcata
    11881 gcgttgtcga tcccgttgcc gcgattgaaa atgcggaaat tgtgattgtc ggcggcggta
    11941 atactttcca gttgctgaaa cagtgccgcg agcgcgggct gctggcaccc attactgacg
    12001 tggtgaaacg tggcgcgctg tatattggct ggagcgcagg cgctaacctt gcttgcccaa
    12061 ccattcgtac caccaacgat atgccgattg tcgatccgca aggtttcgat gcgctaaatc
    12121 tgttcccgct acaaatcaac ccgcacttca ccaacgcgct gccggaaggc cataaaggtg
    12181 aaacccgtga gcagcgtatt cgcgaactgc tggtcgtcgc gccagaactg acgattattg
    12241 gtctgccgga aggtaactgg atcacagtaa gtaaaggtca cgctacgctg ggtggcccga
    12301 acaccactta tgtgtttaag gctggtgaag aagcggtgcc gctggaagcg ggtcaccgtt
    12361 tttaagtgaa ttgccggatg catgatccgg catcttgttc atagtgcctg atgcgacgct
    12421 ttacgcgtct tatcaggcct tcagggtcac ggtattaata ctcgtcccgt ccttcatctt
    12481 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca
    12541 tatctcccag taagcctaaa tgtagcgaac tggtttcaat actctgcacg ttttgctggt
    12601 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc
    12661 tgcggcgcaa acgacgggcg ctggtaacat cgccagagaa gaaaaccgac attgccagtt
    12721 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa
    12781 atgctcgcct tgctgccagc gatttgtcgg caatttcact ccccatgcgc tcgacgatat
    12841 cggaggcctg ttcaaggttg agcgacattt cgatgatctc cgcccagcgg cgcgactctt
    12901 cttctgccag ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa
    12961 cgttgatgtc atcagccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
    13021 ctttattcaa cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcggg
    13081 cagcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
    13141 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
    13201 ggtcaacgaa tggcaacatg accaggcagc gtacaaggtt gtagaagacg tggaaataga
    13261 tcaccaattc cgcttttggc aatggcaact tccccattgt ctctgccagc aaatgaacaa
    13321 acggcaggat aatcaggcta cccaccagct taaacagcag gctacccagc gcgacacggc
    13381 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggtttg
    13441 ccccaatcac cagacaaagc gccactggga aagagataat gcctgcggcg gtcagagtcg
    13501 cggtcagcag taccgcggca aggctggagt agctgataat ggcgaacatc gcaccaatca
    13561 gcgcatccag caaaatgtcg ccagtcagcg aggcaaagat cacctgaacg ccgtttgcct
    13621 gagtgatcgg cgttacggcc tgcacaatca actccagcgc cagtaaaatc agcccaagac
    13681 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
    13741 cgccgataaa aataagtaac ggtgacagcc aggataaatc gaaggtgaga atacgcgcca
    13801 tcagcgccgt cccgacatct gcacccagca caatcaccag cgcaggcgcg agagcaacca
    13861 gatcctgagc gacgaacgag gtcaccagca tggtggtggc attactgctc tgcaccagtg
    13921 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
    13981 cagtacgcag acgcgcgccg aagacgcgca ttacgcctgt tcgaacaata tgagtccccc
    14041 agaccagcag ggcgacggca gaaagcaggt gaagcaaagt tagcacggaa tcaggttctc
    14101 ctttttgtta tgcattttcc tgttaagatt tttctaatca cgttgttttt atccaggcat
    14161 ttctcttgta tgtactttca gtataagaga tttatacaag aaagcattta aaaaggcatt
    14221 tatataaaaa tccacctgta tctgttgcta tttctgtcac actttcccct gtcgtttaca
    14281 ccagataatc gttgtcactt ttgtgacctc tgagaagata aacaacgaaa tatttaagac
    14341 cagatcctgt gaataaaaat aattaagtaa caacggtatt acggagattg aacagcttac
    14401 ggtagcaaaa cgcataaact cctgaatcgc caaaaaagag aaactaaata atttttgtct
    14461 taaaaaacat tgcgcagaaa aaaataaaga gaacaataat actatttgta gcgtaacagt
    14521 ggcgactgca taagcgtaaa aaccgccttc aacaccgata tccttaagta aagaaagtga
    14581 atgaacgata tcggaataaa aagggatacg atcattacca agcgagtaga acagatatgt
    14641 ggctatcgcg ataacatcca taacacccca aaaaagaaga atgtattttt tatattgacg
    14701 ttgcataaag taaatccttt ttacgaaaac aaatattacc aggaattatt ataattgcgg
    14761 gcgtttttgg attaacactc cacccgtatt ttttaaacct ctgtcagcta taccttcact
    14821 aaacaagaca tcgatacgcc ctttactttt atcgtgatac agaatactgc atgagataca
    14881 ccttataccc tctttggatt tacgaaaatc taaccaataa cgaccatcat aaaataccca
    14941 ctccacacct gtttttatat
//
LOCUS       DAZFRE010000108        14810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFRE010000108 DAZFRE010000000
VERSION     DAZFRE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.108"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            235..576
                     /gene="symE"
                     /locus_tag="ACHKUI_004081"
     CDS             235..576
                     /gene="symE"
                     /locus_tag="ACHKUI_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO6600166.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(623..1786)
                     /locus_tag="ACHKUI_004082"
     CDS             complement(623..1786)
                     /locus_tag="ACHKUI_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO6600167.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSQ
                     EDFLNIIRLIESYVFRRAVCAIQTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVYRAQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1840..2716)
                     /locus_tag="ACHKUI_004083"
                     /pseudo
     CDS             complement(1840..2716)
                     /locus_tag="ACHKUI_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2814..2894)
                     /locus_tag="ACHKUI_004084"
     CDS             complement(2814..2894)
                     /locus_tag="ACHKUI_004084"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600168.1"
                     /translation="MQTRCVLQQVRDERLKDIGLCREDVV"
     gene            complement(3122..4042)
                     /locus_tag="ACHKUI_004085"
     CDS             complement(3122..4042)
                     /locus_tag="ACHKUI_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6600169.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLAIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPDREHDSWLES"
     gene            4227..5507
                     /gene="yjiN"
                     /locus_tag="ACHKUI_004086"
     CDS             4227..5507
                     /gene="yjiN"
                     /locus_tag="ACHKUI_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6600170.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALILESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLNADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            5623..6774
                     /gene="yjiM"
                     /locus_tag="ACHKUI_004087"
     CDS             5623..6774
                     /gene="yjiM"
                     /locus_tag="ACHKUI_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6600171.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            6784..7551
                     /gene="yjiL"
                     /locus_tag="ACHKUI_004088"
     CDS             6784..7551
                     /gene="yjiL"
                     /locus_tag="ACHKUI_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6600172.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDNGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQKF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            7548..7805
                     /gene="ytiA"
                     /locus_tag="ACHKUI_004089"
     CDS             7548..7805
                     /gene="ytiA"
                     /locus_tag="ACHKUI_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6600173.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYGVSPR"
     gene            7870..8730
                     /gene="yjiK"
                     /locus_tag="ACHKUI_004090"
     CDS             7870..8730
                     /gene="yjiK"
                     /locus_tag="ACHKUI_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6600174.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            8798..9976
                     /gene="yjiJ"
                     /locus_tag="ACHKUI_004091"
     CDS             8798..9976
                     /gene="yjiJ"
                     /locus_tag="ACHKUI_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600175.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIVGSTLIMQHTRHPFVLAALFFGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLSNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(9989..10543)
                     /gene="kptA"
                     /locus_tag="ACHKUI_004092"
     CDS             complement(9989..10543)
                     /gene="kptA"
                     /locus_tag="ACHKUI_004092"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO6600176.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            10793..11476
                     /gene="yjiH"
                     /locus_tag="ACHKUI_004093"
     CDS             10793..11476
                     /gene="yjiH"
                     /locus_tag="ACHKUI_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6600177.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11473..11934
                     /gene="yjiG"
                     /locus_tag="ACHKUI_004094"
     CDS             11473..11934
                     /gene="yjiG"
                     /locus_tag="ACHKUI_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6600178.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11947..13119
                     /gene="iadA"
                     /locus_tag="ACHKUI_004095"
     CDS             11947..13119
                     /gene="iadA"
                     /locus_tag="ACHKUI_004095"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6600179.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13184..14095
                     /gene="hypT"
                     /locus_tag="ACHKUI_004096"
                     /gene_synonym="qseD"
     CDS             13184..14095
                     /gene="hypT"
                     /locus_tag="ACHKUI_004096"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO6600180.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(14088..14480)
                     /gene="iraD"
                     /locus_tag="ACHKUI_004097"
     CDS             complement(14088..14480)
                     /gene="iraD"
                     /locus_tag="ACHKUI_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6600181.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14477..14560)
                     /gene="idlP"
                     /locus_tag="ACHKUI_004098"
     CDS             complement(14477..14560)
                     /gene="idlP"
                     /locus_tag="ACHKUI_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6600182.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14676..>14810)
                     /gene="ytiD"
                     /locus_tag="ACHKUI_004099"
     CDS             complement(14676..>14810)
                     /gene="ytiD"
                     /locus_tag="ACHKUI_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO6600183.1"
                     /translation="YAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS
                     "
BASE COUNT         3582 a         3777 c         3626 g         3825 t
ORIGIN      
        1 cggcgataaa ctaattttcc cgccctcaaa aaagcaataa agcggctcaa tagccgcttt
       61 attcacatca gcaaaaatta tatttcacgc taattttcat cttattttca ttaattttta
      121 gctggacttt ccctgtattt actgatgata tgtccaggta tttagcgcgg tgcggatgtg
      181 cgccaacaca cccgcaccgc taatcacaat cactatttat agggaatagc agttatgact
      241 gacacgcatt ctattgcaca accgttcgaa gcagaagtct ccccggcaaa taaccgtcaa
      301 ttaaccgtga gttatgcgag tcgctacccg gattacagcc gtattcccgc catcaccctg
      361 aaaggtcagt ggctggaagc cgccggtttt gccaccggca ctgcggtaga tgtcaaagta
      421 atggaaggct gtattgtcct caccgcccaa ccacccgccg ccgccgagag cgaactgatg
      481 cagtcgctgc gccaggtgtg caagctgtcg gcgcgtaaac aaaggcaggt gcaggagttt
      541 attggggtga ttgcgggtaa acagaaagtc gcctgataca ttgacccggt agcgccagtg
      601 ctaccgggta ttattcgcca cgttatacca acgcgctctc catctgtttt tcaaatgcct
      661 gacgaattaa tcccataatg taaggaagtt gtgcgaggtc actgaaacca atttccacat
      721 caccattgcc ccatcgccca acatttgtca catctttggc aatacctttc ggatcgacca
      781 gttcatgaaa ctgcatatta agcgacaacc gcagtcggct tttttgcggt acaacatcaa
      841 caaaattcgt ttcagcttta aacgcaatat agagctttaa aacttcctgc gtgatccctg
      901 cgtccaggcg cataacttca tcgcgaagat gatcaaataa cacccggcta tgcgaaccat
      961 cagcaagaaa aggataatca ctcaggctgt aactggtttt gttctccggc tgcgcccgat
     1021 aaacagctaa aacctcttca ggaagagcag gcgcttgcca gacctgaacc gcgagatcgg
     1081 ccagacgatc tgcacgggcg cgaatggctg cttcatccca tttttcgcac tgtccaagac
     1141 cgatattcaa atagagcggg ctatgtttaa agccgtcttc aatatcgcgt ttttccgcga
     1201 aaggtctgtc actgtagcgg gagttatagc gcgttaatgt gagattcccc aacgtatgca
     1261 acagttcttt atgaatgcgt tgccagtcac ttcccagctc ttcacgccat ttagccgaca
     1321 aattttcgtt ctgcggcata atatgctcaa tcgtaaactc ttccacgcgc tctctgcgtt
     1381 tatcgttttc caggcgtcgt aaccagtagc tgcgactgcg gaagttatag agatcgcgaa
     1441 cttttaattc ccgtttaaat tcatcatcgt tggggaaacg acgatatgaa ggcaggttca
     1501 taaaatgtac ctgaatgctt tccagatatt tttctttatt aatgacctta taaaaagtgg
     1561 caaacgtctt gttcagagaa tttgtctgaa tcgcacacac tgcacggcgg aaaacataag
     1621 attcaattaa acgaattatg ttcaggaaat cttcttgaga caaaacgcca tttttatagt
     1681 catgataaag cgccagtaag aaaggatacg ccacatcgac ctttaactcg cgcaaatcct
     1741 gaaaagccgt ggcaagcgat ttgtcacttt ctttacccaa tgccattgca cagtaatact
     1801 ccgcgtagat gtgaatatct tcaaccagcc gatccacacc tttttcggca acactcgggc
     1861 tacgagcatg cagtttaaat gcctcataga cttcatctgt ccgagggatc tcccccgttt
     1921 ttaccgtcag ataatgacgc ataaaactgt caaaatattc gctgtaacct tgctgaccaa
     1981 aagcgacttc cattggacgc cagtgatctt catacaaccg ggtttgatgc tctggttcga
     2041 gacccatcag aataaaattg cgcaccagat cggcctgact taacgcctta ccagtagagt
     2101 tcatactttc aaaaatcagt tgcggattat cctgaccacg attaagcgcc acatcgacaa
     2161 ttaataactt tgctaaccca cgacataagg ggatcaagtc atcaccgagt ttggcaattt
     2221 gctcatcaaa gaaagtaaag ttttccatta ttcgatggga atagttttct ggcattggtc
     2281 tttgttttat taacgccagc aaactgtctt tatcagtctc agtcagcaat aatttatatc
     2341 ctttctcgcc ggactcataa ggattgagca aataataatt acgcaatttc attgccgaga
     2401 agccatcaaa aacttcttct tcgccaagat ggcgtgataa agcctcaatc agcaacattg
     2461 cggtcgtcag ccgctgttga ccatcaatga ccagtagcgg agagatacca gaaacctgat
     2521 acaacccctg ctcaatataa acaaccgaac cgataaaatg tgctgatata tcgtcacgct
     2581 ttccggcccg aatgatatcg tcccaaagtt gacgacattg ttgcccggtc catgaatagg
     2641 tgcgttgata aatgggaata acaaactgct gcgaacattt taaaaaatcg agtaggcggg
     2701 cttctgtagc tttcattctg acgtctttcc gtgaagcgat gatttcgcaa taataacccc
     2761 ttatctgacg aggtgacata taaatcccat cgtttcgtga aggactctct ccattacacc
     2821 acatcctccc tgcataaccc aatatccttt aaccgttcat ctctcacctg ctgtaacacg
     2881 caccgggtct gcatttgctg ccaccagcag cgttaatgta ccgttcacat aaatggcggc
     2941 ggcaacgttg gtgcaggaag gtcactatca tcgccacgta aagtgtgctt cgttcatgcc
     3001 ggatgcggcg tgaacgcctt atccggccta caaaccgcgc aaattcaata gattgcagag
     3061 ataatgtagg tctgataagc gtagcgcatc aggcagcttt gcgtttgtca tccgtctctg
     3121 attagctctc gagccatgaa tcgtgctcac ggtcaggcaa gggctgctct aatgcctgta
     3181 tttgctctgc cgaaagtccg gtaatctttt gtatccattc aggatctacc ccattctgca
     3241 aaaacaatcg aagaatgcgc tgttcccctt ttatatatcc atcattatga attcgctctg
     3301 caatcgtcat tattcgctcc ctttgttgtg gcagtcgacg ggtaatttca ctgataaact
     3361 cattaaaacg cgcttcatca ccagtcagta aaatgtaatt taacagcgca gttatctggc
     3421 tgtcattagc acactctgta accagtaata ctaccagttg gtcaataagc cccatcagat
     3481 cgcgctggcg aatatgcttt tggatcaact ccaacagggc gactctgcga tgctgcacaa
     3541 tctcgtcgtc tggcacgaca gtgatatcca ccagcgggaa cgcggcggta taaagcttcc
     3601 gtgcggtagt cgggtcggca aattcgtcca gccagcacaa agaccaggga taagggctac
     3661 ggctaccgtg ataaaacagc atcgggatgg ccagcggtag ctgccggcgt ttatcatgtt
     3721 cgatatggcg ctgcatcacc gccatggaat atcgcatcag acgaaaggcc atatgaatat
     3781 cctcacggct ctgatgttca atcactacat aaatatagcc atcgccttca cgggtcttta
     3841 ccgaccacag aatatcggaa tgtagcgccc gcaatttttc atcgacaaaa ctggcggatt
     3901 ccagttttaa gctatcgaga tcgcacagtt cacgtaaatc tttgggtaag tggatctcca
     3961 taaaatcccg cgcggtgtca gagtgtgtga gaaaggattt aaataatgcg tcatgcggcg
     4021 tgctggtcgt aaagtttgtc atggcgatcc gtcacctgta gataagatgg cccaacttta
     4081 gcgactaaca aagccattca cacctggcta tttcctattc cccgaggcgg tttccaggct
     4141 atttttattc ttctgcaaac gatacatttt tatggaagat atctcgcttt ttgcgttttc
     4201 ttaccgtcag gctctattct tacgatatga ataaactcat tgaactcaga cgcgccaaaa
     4261 tgttggcgct ctctttactg cttatcgccg ctgctacctt tgtcgttacg ctgtttttgc
     4321 cgcccaattt ttgggtgagc ggcgtgaagg cgattgctga agcggcgatg gtcggcgcac
     4381 tggcggactg gtttgcggtg gtggcgctgt ttcgccgcgt gccgattccg atcatttctc
     4441 gtcatacggc gattatcccg cgtaataaag accggattgg cgaaaatctc ggccagttcg
     4501 tgcaggaaaa atttctcgat acccaatcgc tggtggcatt gattcgacgt cacgaaccgg
     4561 cgttgttgat tggcaactgg tttagtcagc cggagaacgc ccgccgcgtt ggtcagcatc
     4621 tgttacagat catgagcggt tttctcgaac tgaccgatga tgcgcgtatt cagcgcctgc
     4681 ttaagcgcgc ggtccaccgg gcgatcgata aggtcgatct ttccggcacc agcgcactga
     4741 ttctggagag catgaccaga aacgatcgtc atcaggtgct gctcgatacg ctgatcgcac
     4801 agttgatcgc ccttctccag cgcgataaat cgcgcaagtt tatcgcccag caaattgttc
     4861 gctggctgga gagcgagcat ccactgaaag ccaaaatttt gcccactgaa tggctgggcg
     4921 aacatagcgc ggagttggtt tctgacgcgg tgaattcttt gcttgatgat atcagccgcg
     4981 atcgtgcgca tcagatccgc catgcgtttg atcgcgccac ttttgccctg atcgacaagc
     5041 tgaaaaacga tccggaaatg gcagcgcgag ccgatgccgt aaaaagctat ctgaaagaag
     5101 atgaagcttt taaccgctat ctcagtgaat tgtgggggga tttacgggaa tggctgaatg
     5161 cggatatcaa cagtgaagat tctcgtgtga aagaacgtat cgcgcgggcg ggtcaatggt
     5221 ttggcgaaac gttaattgcc gatgatgcct tgcgggcgtc gttaaatggt catctggaac
     5281 aagccgcaca ccgcattgcg cctgagtttt ccgcattcct gactcgccat atcagcgaca
     5341 cggtaaaaag ctgggacgca cgggatatgt cgcggcaaat cgagttaaat atcggcaaag
     5401 atctccagtt tatccgtgtc aacggtacgc tggttggcgg ttgtattgga cttattttgt
     5461 atttgctgtc gcagctcccg gccttgttcc ccctcggcaa tttatagaaa tccatgaaaa
     5521 actaagtggc taaccgcgag agagatcaaa taaaccacac taaaagaatg gtaataatgt
     5581 ggcctctttt cattatctcc cgtggtacgg ggaaggaaaa tcatgtcact tgtcaccgat
     5641 ctacccgcca ttttcgatca gttctctgaa gctcgccaga aaggctttct caccgtcatg
     5701 gatctcaagg agcgcggcat tccgctggtt ggcacctact gcacctttat gccgcaagag
     5761 atcccgatgg cagccggtgc ggttgtggtt tcgctatgtt ccacctctga tgaaaccatt
     5821 gaagaagccg agaaagatct gccgcgcaac ctctgcccgc tgattaaaag cagctacggc
     5881 ttcggcaaaa ccgataaatg cccctacttc tacttttctg atctggtggt cggtgaaacc
     5941 acctgcgacg gcaaaaagaa aatgtatgaa tacatggcag aatttaagcc cgttcatgtg
     6001 atgcagttgc caaacagcgt taaggacgat gcctcgcgtg cattatggaa agccgagatg
     6061 ctgcgcttgc aaaaaacggt agaagaacgt tttgggcacg agattagcga agatgctctg
     6121 cgcgatgcca ttgcgctgaa aaaccgcgaa cgtcgcgcac tggccaactt ttatcatctt
     6181 gggcagttaa atcctccggc gcttagcggc agcgacattc tgaaagtggt ttacggcgca
     6241 accttccggt tcgataaaga ggcgttgatc aatgaactgg atgcaatgac cgcccgcgtt
     6301 cgtcagcagt gggaagaagg ccagcgactg gacccgcgtc cgcgcatttt aatcaccggc
     6361 tgcccgattg gcggcgcagc agaaaaagtg gtgcgcgcga ttgaagagaa tggcggctgg
     6421 gttgtcggtt atgaaaactg caccggggcg aaagcgaccg agcaatgcgt ggcagaaacg
     6481 ggcgatgtct acgacgcgct ggcggataaa tatctggcga ttggctgctc ctgtgtttcg
     6541 ccgaacgatc agcgcctgaa aatgctcagc cagatggtgg aggaatatca ggtcgatggc
     6601 gtagttgatg tgattttgca ggcgtgccat acctacgcgg tggaatcgct ggcgattaaa
     6661 cgtcatgtgc gccagcagca caacattcct tatatcgcta ttgaaacaga ctactccacc
     6721 tcggatgtcg ggcagctcag tacccgtgtc gcggccttta ttgagatgct gtaaggagtg
     6781 gcagtggcat attcgattgg cattgattcc ggctcaaccg ccaccaaagg gatcttactg
     6841 gcagacggcg tgattacgcg ccgtttcctc gttccaaccc cctttcgccc ggcaacagca
     6901 attactgaag cctgggaaac tctgcgcgaa gggttagaga caacgccgtt tctgacgctc
     6961 accggctacg ggcggcaact ggtggatttt gccgataaac aggtaacgga aatctcctgt
     7021 cacgggctgg gcgcacggtt tcttgcgcca gcaacgcgcg cggtaatcga catcggtggt
     7081 caggacagca aagtgattca gcttgatgat aacggtaacc tgtgcgattt cctgatgaat
     7141 gacaaatgcg cggcgggcac cgggcgtttc ctggaggtga tctcgcgcac gcttggcacc
     7201 agcgtcgagc aactcgacag cattaccgaa aatgtcacgc cgcacgccat cacgagtatg
     7261 tgcacagtgt ttgctgaatc agaagcgatc agcctgcgct cagcgggcgt cgcgccagaa
     7321 gcgattctcg ccggagtgat taacgcgatg gcgcggagga gtgccaattt cattgctcgt
     7381 ctctcctgtg aagcgccgat tctgtttact ggtggcgtta gtcattgcca gaagtttgcc
     7441 cggatgctgg aatctcacct gcgaatgccg gtaaataccc atcctgatgc gcaatttgct
     7501 ggcgcaattg gcgcggcggt aattggtcaa cgagtgagga cacgccgatg aaagagtttt
     7561 tatttttatt tcactccacg gtcggcgtca tacaaacccg caaagcgttg caggcagcgg
     7621 gcatgacttt tcgcgtcagc gatattcccc gtgatttacg cggcggctgc gggttatgca
     7681 tttggctgac ctgtcccccc ggcgaggaaa tacaatgggt gatccctggg ctgactgagt
     7741 caatttattg ccagcaggat ggtgtctggc gctgcattgc acattacggg gtttctccgc
     7801 gttaaggatt cattaaggtc gctacgtata cttctcgata tcttattgat ttgccttaaa
     7861 gggattaccg tgacaaaaag tatttcgtta agtaaacgca tctccgttat tgtgattctt
     7921 tttgccattg tggcagtgtg tactttcttc gtgcaatctt gcgcccgtaa aagcaatcat
     7981 gcagcatcat ttcagaatta ccacgccacc attgacggca aagaaattgc aggcataacg
     8041 aataatatct cttcgctgac ctggtcggca caaagcaata ctctttttag cactatcaac
     8101 aaacctgccg ccattgttga aatgaccacc aatggtgatt ttatccgcac aattcctctg
     8161 gattttgtta aggatctcga gacgatcgaa tacattggcg ataatcagtt tgtcattagc
     8221 gatgagcgcg attacgccat ttatgtgatt tcactgaccc caaactcgga agtaaaaatc
     8281 ctcaaaaaaa taaaaatccc gctgcaagac tccccaacca attgcggttt tgaagggttg
     8341 gcttattctc gccaggatca cactttctgg ttttttaaag agaaaaatcc gattgaggtc
     8401 tacaaagtta acgggctgtt aagtagcaat gaattacaca tcagcaaaga cgaggcacta
     8461 caacggcaat ttactttaga tgatgtgtcc ggcgcagaat ttaaccagca aaaaaatacg
     8521 cttctggtgc tgtctcatga atctcgcgca ctccaggaag taaccctggt tggcgaggtt
     8581 atcggtgaaa tgtcactaac caaaggcagt cgaggtttgt cgcataacat caaacaggcc
     8641 gaaggggttg cgatggatgc ctctggtaat atttatatcg ttagcgaacc aaatcgtttt
     8701 tatcgcttta cccctcaatc atcgcattaa caggctggca tgcttcacct ttttgttatt
     8761 aacttgttaa caacaaaaat gacaaacaag gttaaacatg ccttcgtcca cgcatcccgt
     8821 agaacgcttt tctttcagca ccgcgctttt cgggatgctg gttctgacct taggtatggg
     8881 tttaggccgc ttcctttata cgcctatgtt gcccgtcatg atggcggaag gatcgttttc
     8941 atttagccag ctctcgtgga ttgccagcgg caactatgcg gggtatctgg ctggcagtct
     9001 gctattttct tttggcgcat ttcaccagcc atcgcgcctg cgcccatttc tgttggcttc
     9061 tgccctggcg agcgggttgt tgatcctcgc aatggcatgg ttgccgccat ttattctggt
     9121 gttattgatt cgcgtcctgg cgggtgtcgc cagcgccggt atgctgattg ttggttcgac
     9181 gctgattatg cagcacacgc gccatccttt tgtgctggca gctttgtttt ttggcgttgg
     9241 cattggcatc gcactgggca atgaatatgt tctggcaggc ctgcattttg acctctcctc
     9301 gcaaacgtta tggcaaggcg ccggggcgct ttctggcatg atgctgattg cacttacgct
     9361 tttaatgccc tcgaaaaaac acgccatcac accaatgcca ttggcaaaaa cggagcaaca
     9421 gataatgagc tggtggttac tggctattct gtacggcctg gcgggatttg gttatatcat
     9481 cgtcgcgact tacctgccgc tcatggcgaa agatgcaggc tcaccattgt taaccgccca
     9541 tctctggacg ttagtcggct tatcaattgt gcctggttgc tttggctggc tatgggcagc
     9601 aaaacgttgg ggggctttgc cctgcctgac ggcgaatttg ctggtgcagg ctatctgtgt
     9661 gctgcttact ctcgccagcg actcgcctct cttgcttatc atcagcagtc ttggttttgg
     9721 cggtaccttt atgggcacga catctctggt gatgactatc gcccgccaac tgagcgtacc
     9781 gggaaatctc aatcttttag gctttgtgac gctcatttac ggcattgggc aaatcctcgg
     9841 cccggcgctg accagtatgc tcagcaatgg aacatcggcg cttgccagcg ccactctctg
     9901 cggcgcggcg gcgttgttta ttgctgcgct tatcagcaca gtgcaattat ttaagctaca
     9961 agtggtcact tcatgaggga agtttccctt accactcaag aaactcaacc gcaaccgttg
    10021 atgtcagcca gaccccgttt tccgcttgcc agaaagggag gcctcgtttc gccatttcct
    10081 gtgctttgac ggttaaaata accggcgatc cgtgtcttgc tcccactttg cgtgccgttg
    10141 cttcatcagc agagagatgt acgtagtgac gctcacctgc aatcagcccc tgttttttta
    10201 tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg
    10261 caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg cacctaccat
    10321 cactggaata actaaaacgc tttttatcgc tggtggcaac cacagtatcc agcaatgcac
    10381 gggttaaccg cttgcccgct ttttgggcac aaagtatgag cttatcgata tccgcccatc
    10441 cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa
    10501 acttgctcgt atccgccagc tctttttcgt tgtactttgc catctcgttt tcgtccctgg
    10561 attttctgtt tttatttact gatgtcattg tcacttggtt tatgaatttt ctgcaactca
    10621 atcctgcgca gagccttttc cacacagcgt aatttttccg ctatgcaaaa aacgcatagc
    10681 cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt cagttccagg
    10741 aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg ttatgacaca
    10801 acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct
    10861 ggcctttttt ctcaccataa tctttttttc cggtgttttt tccggaacag acagctggtg
    10921 gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaacgg
    10981 agccaccacc tcatttcgcg gcgcgggtgg cgcgggggct aaagatggct ttctctttgc
    11041 gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct
    11101 tggtggatta cgcgccgcgc agcagttaat gacacctgtg ctaaaaccgt tgttgggcat
    11161 tccagggatc tgctcgctgg cgctgatcgc taacctgcaa aacaccgatg ccgccgccgg
    11221 tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag tgattttcgc
    11281 cgcatatcaa accagcggta gcgccatcat caccaactac ttctcttccg gcgtcgcggt
    11341 gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt
    11401 tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca acccaacgca
    11461 aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc
    11521 gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt
    11581 gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca tatctgtgaa
    11641 cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta
    11701 atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg caaccgctgg cgcattaacc
    11761 ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc ggtgcaaaat
    11821 gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg
    11881 gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga
    11941 gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc gcatttgtat
    12001 gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt
    12061 gccagcaata tcccttctga cattgtaccg aactgcacgg ttgtcgatct cagtgggcag
    12121 atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg cggcgaagca
    12181 ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc tgacggaagc gggcgtcacg
    12241 tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc cctgctcgcc
    12301 aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat
    12361 gtcccttccc gcaccattac gggttccgtg gaaaaagacg tggcgattat cgatcgtgtg
    12421 attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg
    12481 gccaatatgg cggcagaatc ccgcgttggc ggtttgctcg gcggtaaacc tggcgtcacc
    12541 gtgttccaca tgggcgacag taaaaaggcg ttacagccta tttatgacct gctggaaaac
    12601 tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta accgcaacgt accgttgttt
    12661 gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag cagcattgac
    12721 gaaccggtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat tccgctggca
    12781 cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga cgaagggaat
    12841 ttaacccata tcggtgttgc cggttttgaa acgttgctgg aaaccgtgca ggtgctggtc
    12901 aaagactatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt
    12961 ttccttaacc tgaccgggaa aggcgaaatt ctgccaggca atgatgctga cttgctggtc
    13021 atgacgccag aactgcgcat tgagcaggta tacgctcgcg gcaaactgat ggtcaaagac
    13081 ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa cggttgtcag ccgcccgaat
    13141 ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc
    13201 gattttgcat aatattgaaa ccaaatggct ttatgatttt ctgaccctgg aaaaatgccg
    13261 caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa ccggcattca gccgccgcat
    13321 ccgtgcgctg gaacaggcga ttggtgttga attgtttaac cgccaggtaa cgccgctgca
    13381 actctcagaa caagggaaaa tcttccattc gcagatccgc catctgttgc aacagttaga
    13441 gagcaatctg gcagagctgc gtggcggcag cgattacgcg caacgaaaaa tcaagatagc
    13501 cgctgcacac tctctttccc tcgggctgtt accgtccatt atcagccaga tgccaccgct
    13561 ctttacctgg gcaattgaag ctattgatgt cgatgaagcg gtcgataaac tgcgtgaagg
    13621 gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca
    13681 cattcgctta tttgaatctc aattgttccc tgtctgcgcc agtgacgaac acggagaagc
    13741 actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat
    13801 ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcaccttttt
    13861 tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg
    13921 gctgccggag tacgccatcc aacaagaaat tcgcagcggg aagctcgttg tactgaatcg
    13981 ggacgaactg gtgatcccga ttcaggccta tgcataccgg atgaataccc gcatgaatcc
    14041 cgttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc
    14101 tccagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt tagcggttca
    14161 tcgtcgtggt acagcattat ctcaaggctg atcgccagag tgtggcagta cgcttctttc
    14221 aaattaacct gcaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga
    14281 cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg
    14341 cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt gaggagatcg
    14401 ctgcataccg atttacgcag cgatgacgtt ttattgcctg aaatttcagg taaaacagcc
    14461 tgaagtgatt gtcgcatcat tttgcgcact cctgacgttt agcaacatat gcggcttgcc
    14521 ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat
    14581 tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt
    14641 gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa aatcatcaaa
    14701 ctcaaaaaaa aaccgctagt atattctgaa tattccgaat agttacgtag atgaaaatac
    14761 ttgtcaccgc tgctacttaa ttccggtggg aaattattta tttccgcata
//
LOCUS       DAZFRE010000109        14760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFRE010000109 DAZFRE010000000
VERSION     DAZFRE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.109"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            246..1007
                     /locus_tag="ACHKUI_004100"
     CDS             246..1007
                     /locus_tag="ACHKUI_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6600184.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            1027..2520
                     /locus_tag="ACHKUI_004101"
     CDS             1027..2520
                     /locus_tag="ACHKUI_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6600185.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLANTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            2649..3908
                     /locus_tag="ACHKUI_004102"
     CDS             2649..3908
                     /locus_tag="ACHKUI_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO6600186.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSSSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            4143..5309
                     /gene="nhaA"
                     /locus_tag="ACHKUI_004103"
     CDS             4143..5309
                     /gene="nhaA"
                     /locus_tag="ACHKUI_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6600187.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5369..6274
                     /gene="nhaR"
                     /locus_tag="ACHKUI_004104"
     CDS             5369..6274
                     /gene="nhaR"
                     /locus_tag="ACHKUI_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6600188.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(6370..6633)
                     /gene="rpsT"
                     /locus_tag="ACHKUI_004105"
     CDS             complement(6370..6633)
                     /gene="rpsT"
                     /locus_tag="ACHKUI_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6600189.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            6736..6954
                     /locus_tag="ACHKUI_004106"
     CDS             6736..6954
                     /locus_tag="ACHKUI_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6600190.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            6962..7903
                     /gene="ribF"
                     /locus_tag="ACHKUI_004107"
     CDS             6962..7903
                     /gene="ribF"
                     /locus_tag="ACHKUI_004107"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6600191.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            7946..10762
                     /gene="ileS"
                     /locus_tag="ACHKUI_004108"
     CDS             7946..10762
                     /gene="ileS"
                     /locus_tag="ACHKUI_004108"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6600192.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKNVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            10762..11256
                     /gene="lspA"
                     /locus_tag="ACHKUI_004109"
     CDS             10762..11256
                     /gene="lspA"
                     /locus_tag="ACHKUI_004109"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6600193.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            11364..11813
                     /gene="fkpB"
                     /locus_tag="ACHKUI_004110"
     CDS             11364..11813
                     /gene="fkpB"
                     /locus_tag="ACHKUI_004110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6600194.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            11815..12765
                     /gene="ispH"
                     /locus_tag="ACHKUI_004111"
                     /gene_synonym="lytB"
     CDS             11815..12765
                     /gene="ispH"
                     /locus_tag="ACHKUI_004111"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6600195.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            12831..13745
                     /gene="rihC"
                     /locus_tag="ACHKUI_004112"
     CDS             12831..13745
                     /gene="rihC"
                     /locus_tag="ACHKUI_004112"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6600196.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            13912..14733
                     /gene="dapB"
                     /locus_tag="ACHKUI_004113"
     CDS             13912..14733
                     /gene="dapB"
                     /locus_tag="ACHKUI_004113"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6600197.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         3623 a         3394 c         3907 g         3836 t
ORIGIN      
        1 acgttcagca gctaatgcta agttcatcat gaaaaatgcc tataaatcaa agggtgatgt
       61 ttaaaacaga cacattttct gaaacacgat atccctccgc ccgttcgcaa aagtaatctg
      121 cgatcttcat cacgcttcga tagtcatctt taaatatact ggcctggtca ttcttaataa
      181 aaatgaattg tctggtgtat taattcctat ggaatcataa aaacaatgaa tggagaacat
      241 attatatgtc tgccaaaaga cgacttctta ttgcgtgtac cttgataaca gctatctatc
      301 attttcctgc atattcttca ttagaatata aaggaacctt tggttcaata aatgcgggtt
      361 atgcagactg gaatagtgga tttgtaaaca ctcaccgtgg tgaagtatgg aaagtgactg
      421 cggattttgg ggtaaatttt aaagaagcag aattttactc attttatgaa agtaatgtac
      481 tcaatcatgc tgtagcaggg agaaatcata cggtttcagc aatgacgcat gtcagactct
      541 ttgactctga tatgacattc tttggcaaaa tttatggcca atgggataac tcatggggtg
      601 acgatctgga catgttttat ggattcggtt acctcggctg gaacggcgag tggggctttt
      661 ttaaaccgta tattggattg cataatcaat ctggtgacta cgtatcagct aaatatggtc
      721 aaacgaatgg ttggaatggt tatgttgttg gctggacagc agtattacca tttacgttat
      781 ttgacgaaaa atttgtttta tctaactgga atgaaataga actggacagg aacgatgctt
      841 acacggagca gcaatttggc cggaacgggt taaatggcgg tttaactatt gcctggaagt
      901 tctatcctcg ctggaaagca agtgtgacgt ggcgttattt cgataataag ctgggctacg
      961 atggctttgg cgatcaaatg atttatatgc ttggttatga tttctaattt ttatcaggga
     1021 ataattatgc agaaaacgtt aatggccagt ttgatcggcc ttgctgtttg cacagggaat
     1081 gcttttagtc ctgccttagc cgcagaggct aaacaaccta atttagtcat tattatggcg
     1141 gatgatttag gttatggcga tttagcaaca tatggtcatc agatcgttaa aacacctaat
     1201 atcgacaggc ttgcccagga aggggtcaaa tttactgact actatgcccc cgctccttta
     1261 agttcacctt cacgcgcagg gctattaacc ggccggatgc catttcgtac tggaattcgc
     1321 tcatggattc cttcaggcaa agatgttgcc ttagggcgta acgaactcac gattgctaat
     1381 ctactcaaag cgcaagggta cgacacggca atgatgggta agctgcatct gaatgcaggc
     1441 ggcgatcgca ccgatcagcc acaagcacaa gatatgggct ttgattactc actggctaat
     1501 acggcgggct ttgttaccga cgccacgctg gataacgcta aagaacgccc gcgttatggc
     1561 atggtttacc cgacaggctg gctacgtaat gggcaaccca ctccacgagc cgataaaatg
     1621 agcggtgagt atgtcagttc ggaagtcgtc aactggctgg ataacaaaaa ggacagcaag
     1681 cctttcttcc tctatgttgc ttttaccgaa gtgcatagcc ccctggcttc gcccaaaaaa
     1741 tacctcgaca tgtactcaca atatatgagc gcgtatcaga agcagcatcc tgatttattt
     1801 tatggcgact gggcagacaa accctggcgt ggtgtggggg aatattatgc caatatcagc
     1861 tatctggatg cacaggttgg aaaagtgctg gataaaatca aagcgatggg tgaagaagat
     1921 aacacaatcg ttatttttac cagtgataac ggtccggtaa cgcgtgaagc gcgcaaagtg
     1981 tatgagctga atttggcagg ggaaacggat ggattacgcg gtcgcaagga taacctttgg
     2041 gaaggcggaa ttcgtgttcc agccattatt aaatatggta aacatctacc acagggaatg
     2101 gtttcagata cacccgttta tggtctggac tggatgccta ctttagcgaa aatgatgaac
     2161 ttcaaattac ctacagaccg tactttcgat ggtgaatcgc tggttcctgt tcttgagcaa
     2221 aaagcattga aacgcgaaaa gccattaatt ttcgggattg atatgccatt ccaggatgat
     2281 ccaaccgatg aatgggcgat ccgtgatggt gactggaaga tgattatcga tcgcaataat
     2341 aaaccgaaat atctctacaa tctgaaatct gatcgttatg aaacacttaa tctgatcggt
     2401 aaaaaaccag atattgaaaa acagatgtat ggtaagtttt taaaatataa aactgatatt
     2461 gataatgatt ctctaatgaa agccagaggt gataaaccag aagcggtgac ctggggctaa
     2521 cctgataatc ataataaatg taatgtatgc catttagggc tatgcccgcc tgattcagtg
     2581 ctacgcattg aatcaggttc ttttcgcgtt gaacaatatc aagatacggc tattcgcagg
     2641 gagtggatat gtttaccaac gtaaacgttg attgttgcaa aacaccagga tgtaaaaacc
     2701 tggggttgct gaatagccag gattatgtcg cacagggtaa aaatatttta tgccgtgaat
     2761 gtggttactt gtttccagtg atatctgaac agtcgcttaa tatttatcgt aatattgtga
     2821 atcactcctg gagaggtttg atttgccaat gttcaacttg cggaggcacg tccctcaaaa
     2881 aatatggata ttctgcacaa ggccagagaa gaatgtattg ccatcattgt gagaaaacat
     2941 ttatcactct ggaacatgta attaccacac cacgaggagc ccagttagca ttgatgattg
     3001 agcaagggga ggcacttgcg gatatcagaa agtcattact tcttaacagc actggactta
     3061 gccgtgaact gttaaaatta gcgcgtgaag caaactataa agaaagtcga cagtgtttcc
     3121 cagcttctga tattaccctg agtacccgcg cttttcgcgt caagtataat ggtagcaata
     3181 actctcttta tgctcttgtt accgcagaag aacaaagcgg cagggtggtt gccatctcaa
     3241 ccaattactc ctcatctgcc gtagagcaac attatcaata cacatcgaac tatgaagagc
     3301 gtatgtctcc agggacgctg gcacatcatg tccagcgcaa agagttactt actatgcggc
     3361 gggatacctt gtttgatatt gattacggcc cggcagtttt acatcaaaac gatccgggaa
     3421 tgttggtaaa accggttctt ccggcatatc gtcattttga actggtcaga atactgaccg
     3481 atgagcattc caacaacgtt cagcattacc ttgatcacga atgctttata ttgggcggct
     3541 gcctgatggc taatttgcag catattcatc aaggtcgctg ccatatttcc tttgtcaaag
     3601 agcgcggtgt ggcacccgcc accattgatt ttccaccgcg attattcctt agtggtgggg
     3661 tacgaaataa tgtctggcgt gcattttcta accgcaatta ttcaatggct gtatgcaatc
     3721 tcactggcag taagaaagtc cgcgagatgc ggcatgcaac attgaacagt gcgacgcgtt
     3781 ttatccactt tgtggagaac catcctttcc ttatatcatt gaaccgaatg tctcctgcga
     3841 atgtcgtttc tacattagat atcctcaaac atctgtggaa taaaaaacta gagcatggaa
     3901 caatttaatt attacttttc cgaataattt cttcagaaat cttgacatta gactatgatt
     3961 aatggggttt aaggtcatca gccgccgctt tcgggcatca aaaactcatc tctcgctgat
     4021 ggcgcaattc ttttaataga tcgtaaaaag cgagctattc ctacactata atctgatttt
     4081 aacgatgatt cgtgcggggt aaaatcgtga aaacgatcta ttcacctgaa gagaaataaa
     4141 aagtgaaaca tctgcatcga ttctttagca gtgatgcctc gggaggcatt attctcatta
     4201 ttgccgctgt attagcgatg attatggcca acagcggtgc aaccagtgga tggtatcacg
     4261 actttcttga gacgccggtt cagctccggg ttgggacact tgagatcaac aagaacatgc
     4321 tgctatggat caatgacgct ctgatggcgg tatttttcct gttggttggt ctggaagtta
     4381 aacgcgagct gatgcaaggt tcgctggcca gtctgcgcca ggcggcattt cctgttattg
     4441 ccgcaatcgg cgggatgatt gtcccggcat tgctctatct ggcttttaac tatgccgatc
     4501 cgattacccg cgaaggctgg gcaatcccgg cggcgactga cattgccttt gcacttggtg
     4561 tgttggcgct gttgggaagt cgtgttccgt tagcgctgaa gatctttttg atggctctgg
     4621 ctattatcga cgatcttggg gccatcatta tcatcgcatt gttctacact aatgacttat
     4681 cgatggcctc tcttggcgtc gcggctgtag caattgcggt actcgcggta ttgaatctgt
     4741 gtggtgtacg ccgcacgggc gtttatattc tggttggcgt ggtgctgtgg acagcggtgt
     4801 tgaaatcggg ggttcacgca accctggctg gcgtcattgt cggcttcttt attcctttga
     4861 aagagaagca tgggcgctct ccggctaaac gtctggagca tgttttgcat ccatgggtgg
     4921 cgtatctgat tttgccgctg tttgcatttg ctaatgctgg cgtttcactg caaggtgtca
     4981 cgctggaagg tttgacctcc attctgccat tagggatcat cgctggtttg ctgattggca
     5041 agccactggg tattagtctg ttctgctggt tggcgctgcg tttgaaactg gcacatctgc
     5101 cagagggaac gacttaccag caaattatgg cggttggtat cctgtgcggt atcggtttta
     5161 ctatgtctat ctttattgcc agcctggcat ttggtagcgt agatccagaa ctgattaact
     5221 gggcaaaatt aggcatcctt gtcggttcaa tttcttcggc ggtaattgga tatagctggt
     5281 tacgcgttcg tttacgtcca tcagtttgac aggacgatgt actggggagc cataaacggc
     5341 tcccttttca ttgttatcag ggagagaaat gagcatgtct catatcaatt acaaccactt
     5401 gtattacttc tggcatgtct acaaagaagg ttctgtggtt ggcgcagcgg aggcgcttta
     5461 tttaacacca caaaccatta ccgggcagat ccgggcgctg gaagagcgcc tgcaagggaa
     5521 actatttaag cgtaaaggac gtggtctgga acccagcgaa ctgggagaac tggtctatcg
     5581 ctatgccgat aaaatgttca ccttaagcca ggaaatgctg gatatcgtca actatcgcaa
     5641 agagtccaac ttattgtttg atgttggtgt ggcagatgca ctttccaaac gtctggtcag
     5701 cagtgttctg gatgccgcag ttgtggaaga cgagcagatc catctacgct gtttcgaatc
     5761 gacgcacgag atgcttttag agcagttgag tcagcataaa ctggatatga tcatctctga
     5821 ctgtccgatc gattccactc agcaggaagg gctgttttcc atgaaaattg gcgaatgtgg
     5881 tgtcagtttc tggtgcacta acccactacc agaaaagccg tttcctgcct gtcttgaaga
     5941 gcgtcgttta cttattccgg ggcgtcgctc aatgttgggg cgtaaactat taaactggtt
     6001 taactcccag ggcttgaacg tcgaaatttt gggtgagttt gatgatgctg cgttgatgaa
     6061 agcctttggg gcgacgcata acgctatttt cgttgcacct tcgctttacg ctaatgattt
     6121 ctataacgat gactcggttg tggagatagg ccgtgttgag aacgtgatgg aagagtacca
     6181 cgcgattttt gccgaaagga tgattcagca ccctgcagta cagcgtatct gcaatacaga
     6241 ctattctgcg ctgtttactc cagcttcaaa ataaacattt gtactttgca ggccgaattt
     6301 tggcctgcag atggatataa aaaaacccgc cagagcgggt ttttttacaa agctgacaac
     6361 aacaggcaat taagccagtt tgttgatctg tgcagtcagg ttagccttat gacgtgcagc
     6421 tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgattggtt gcatttcgtt
     6481 aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata ctttcttgat
     6541 gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt cagactgaat
     6601 ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg tgttctatat
     6661 ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat ggatttgtgc
     6721 aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg attctaccag
     6781 cttgcggggt gtgaatacag cttttccgcg agaaaaattg cagcaggcgg tcagtttctt
     6841 cccatgattt gcgccatagc aatgaaaagc cacttctttc tgatttcggt actcaatcgc
     6901 cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt ttgagccaga
     6961 catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag ggtgtgtgct
     7021 gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca
     7081 ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac ctcaaccact
     7141 ggaactgttt gctactgata aagccccggc acggctcacc cggctgcggg aaaaactacg
     7201 ttatcttgca gagtgtggtg ttgattacgt gctgtgcgtg cgttttgaca ggcgttttgc
     7261 ggcgttaacc gcgcaaaact tcatcagtga tcttctggtg aagcacttgc gggtaaaatt
     7321 tcttgccgta ggtgacgatt tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt
     7381 acagaaagcg ggcatggaat acggcttcga tatcaccagc acgcaaactt tttgcgaagg
     7441 tggcgtgcgt atcagcagca ccgccgtgcg tcaggcgctt gcggatgaca atctggctct
     7501 ggctgaaagt ttactggggc acccgtttgc tatctccggg cgtgtagtcc acggtgatga
     7561 attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc
     7621 ggtgaaaggg gtttatgcgg tagaagtgtt gggccttggc gaaaagccgt tacccggcgt
     7681 tgcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcaactgg aagtgcattt
     7741 gttagatgtt gcaatggacc tttatggtcg ccatatacaa gtagtgctgc gtaaaaaaat
     7801 acgcaatgag cagcgatttg catcgctgga cgaactgaaa gcgcagattg cgcgtgatga
     7861 attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt atgtaatcaa
     7921 accgaaatac ggaaccgaga atctgatgag tgactataaa tcaaccctga atttgccgga
     7981 aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg
     8041 gactgatgat gatctgtacg gcatcattcg tgcagctaaa aaaggcaaaa aaaccttcat
     8101 tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggttaacaa
     8161 gattctgaaa gacattatca ttaagtccaa agggctttct ggatatgact cgccgtatgt
     8221 gcctggctgg gactgtcatg gtctgccaat cgagctgaaa gtagagcaag aatacggtaa
     8281 gccgggggag aaattcaccg ccgctgagtt ccgcgccaag tgccgcgaat acgctgcgac
     8341 ccaggttgac ggtcagcgca aagactttat ccgtctgggc gtgctgggcg actggtcgca
     8401 cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc tgggcaaaat
     8461 catcggcaac ggtcacctgc acaaaggcgc gaagccggtg cactggtgcg ttgactgccg
     8521 ttctgcactg gcagaagcgg aagttgagta ttacgacaaa acttctccgt ccatcgacgt
     8581 cgctttccag gcggtcgatc aggatgcgct taaaacgaaa tttggcgtaa gcaatgttaa
     8641 cggcccaatt tcgctggtta tctggaccac cacgccgtgg acgctgcctg ctaaccgcgc
     8701 aatctccatt gcacctgatt ttgattatgc gctggtgcaa atcgacggtc aggccgtgat
     8761 cctcgcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgttagcg attacaccat
     8821 tcttggcacg gtgaaaggtg ccgagctgga actgttgcgc tttacccatc cgtttatgga
     8881 cttcgatgtt ccggcaattc tcggcgacca cgttacgctg gatgccggta ccggtgccgt
     8941 tcataccgcg ccaggccacg gtccggacga ctatgtgatc ggtcaaaaat atggtctgga
     9001 aaccgctaac ccggttggcc cggacggcac ttatctgccg ggtacttacc cgactctgga
     9061 tggcgttaat gtcttcaaag cgaacgatat tgtcattgcg ttgttgcagg aaaaaggcgc
     9121 gctgttgcac gttgagaaaa tgcaacacag ctatccgtgc tgctggcgtc ataaaacgcc
     9181 gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc
     9241 gcagtcactg aaagagatca aaggcgtgca gtggatccct gactggggcc aggcgcgtat
     9301 cgagtcgatg gttgctaacc gtcctgactg gtgtatctct cgtcagcgta cctggggcgt
     9361 gccgatgtca ctgttcgtgc acaaagacac agaagaactg catccgcgta ctctcgaact
     9421 gatggaagaa gtggcaaaac gcgttgaagt tgacggcatt caggcgtggt gggatctcga
     9481 tgcgaaagag atcctcggcg acgaagctga ccagtatgtg aaagtaccgg atacgctgga
     9541 tgtatggttt gactccggat ctacccactc ttccgttgtt gatgtgcgtc cggaatttgc
     9601 cggtcacgca gcggacatgt atctggaagg ttctgaccaa caccgtggct ggttcatgtc
     9661 ttccctgatg atctctaccg cgatgaaggg caaagcgcca tatcgtcagg tactgactca
     9721 cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggta acaccgtttc
     9781 gccgcaggat gtgatgaata aactgggtgc ggatattctg cgtctgtggg tggcatcaac
     9841 cgactacact ggcgaaatgg ccgtttctga cgagatcctg aaacgtgctg ccgacagcta
     9901 tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt ttgatccggc
     9961 aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg taggttgtgc
    10021 gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc acgaagtggt
    10081 acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg acatcatcaa
    10141 agaccgtcag tataccgcca aagcggacag cgtggcgcgt cgtagctgcc agactgcgct
    10201 gtatcacatc gcagaagcgc tggttcgctg gatggcacca atcctctcct tcaccgctga
    10261 tgaagtgtgg ggttacctgc cgggcgaacg tgaaaaatac gtcttcaccg gcgagtggta
    10321 cgaaggcctg tttggtctgg cagacagtga agcaatgaac gatgcgttct gggacgagct
    10381 gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg ataagaacgt
    10441 gggcggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgg cggcgaaact
    10501 gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta ccgttgcaga
    10561 ctataacgac gcacctgctg atgcccagca gagcgaagtc ctcaaagggc tgaaagtcgc
    10621 gttgagtaaa gccgaaggtg agaagtgtcc tcgctgctgg cactacaccc aggatgtcgg
    10681 caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg
    10741 tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct acgctggctg
    10801 tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat cctccagaac
    10861 tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac
    10921 tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg gttctttgcc
    10981 ggtattgcga ttggtattag cgtgatcctg gtagtgatga tgtatcgctc gaaggccacg
    11041 cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcactggg caacctgttc
    11101 gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctatgtcgg cgactggcac
    11161 ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg gtgcggcact gattgtgctg
    11221 gaaggttttt taccttctaa agcgaaaaaa caataataaa ccctgccgga tgcgatgctg
    11281 acgcatctta tccggcctac agattgctgc gaaatcgtag gtcgcatccg gcaaaaatcc
    11341 tcaaaatata agagcaaacc tgcatgtctg aatctgtaca gagcaatagc gccgtcctgg
    11401 tgcacttcac gctaaaactc gacgatggca ccaccgctga gtctacccgc aacaacggta
    11461 aaccggcgct gttccgcctg ggtgatgctt ctctttctga agggctggag caacacctgc
    11521 tggggctgaa agtgggcgat aaaaccacct tctcgctgga gccagatgcg gcgtttggcg
    11581 tgccgtcacc ggacctgatt cagtacttct cccgccgtga atttatggat gcaggcgagc
    11641 cagaaattgg cgcaatcatg ctttttaccg caatggatgg cagtgagatg cctggcgtga
    11701 tccgcgaaat taacggcgac tccattaccg ttgatttcaa ccatccgctg gccgggcaga
    11761 ccgttcattt tgatattgaa gtgctggaaa tcgatccggc actggaggcg taacatgcag
    11821 atcctgttgg ccaacccgcg tggtttttgt gccggggtag accgcgctat cagcattgtt
    11881 gaaaacgcgc tggccattta cggcgcaccg atatatgtcc gtcacgaagt ggtgcataac
    11941 cgctacgtgg tcgatagcct gcgcgagcgt ggggctatct ttattgagca gattagcgaa
    12001 gtaccggacg gcgcgatcct gattttctcc gcacacggtg tttctcaggc ggtacgtaat
    12061 gaagcgaaaa gccgtgattt gacggtattt gacgccacct gtccgctggt gaccaaagtg
    12121 catatggaag tcgcccgcgc cagccgtcgt ggtgaagaat ctattctcat cggtcacgcc
    12181 gggcacccgg aagtggaagg gacgatgggc cagtacagca acccggaagg gggaatgtat
    12241 ctggtcgaat cgccagacga tgtgtggaaa ctgacggtca aaaacgaaga gaagctctcc
    12301 tttatgaccc aaaccacgct gtcggtggat gacacgtctg atgtgatcga cgcgctgcgt
    12361 aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca tctgctacgc cacgactaac
    12421 cgtcaggaag cggtacgcgc cctggcagag caggcggaag tagtactggt ggttggttcg
    12481 aaaaactctt ccaactccaa ccgtctggcg gaactggccc agcgtatggg caaacgcgcg
    12541 tttttgattg acgatgcgac agatatccag gaagagtggg tgaaagaggc gaaatgcgtc
    12601 ggcgtgactg ctggcgcatc agctccggat attctggtac agaacgtagt cgcacgttta
    12661 cagcagcttg gcggtggcga agccattccg ctggaaggcc gcgaagaaaa tatcgttttc
    12721 gaagtgccga aagagctgcg agtcgatatt cgtgaagtcg attaagtcat tagcagccta
    12781 agttatgcga aaatgccggt cttgttaccg gcatttttta tggagaaaac atgcgtttac
    12841 ctatcttcct cgatacagac cccggcattg acgatgccgt tgccattgcc gccgcgattt
    12901 ttacccccga actcgatctg caactgatga ccactgtcgc gggtaatgtt tcggtagaga
    12961 aaacgacccg caacgccctg caactgctgc atttctggaa tgtggatatt ccgcttgccc
    13021 agggcgctgc cgtaccactg gttcgtgcgc cgcgcgatgc ggcatctgta cacggtgaat
    13081 cgggaatggc tggctatgac tttgttgaac acaaccgtca gccactgggt ataccggcgt
    13141 ttctggcaat tcgtgatgcc ctgatgcgtg cgccagagcc cgttaccctg gtggccatcg
    13201 gcccgttaac caatattgcg ctgttacttt cacaatgccc ggaatgtaag ccgtatattc
    13261 gccgtctggt gatcatgggc ggttctgctg gtcgcggcaa ctgcacacca aacgccgagt
    13321 ttaatatcgc tgccgatcca gaagcggcag cctgtgtctt ccgtagcggc atcgaaatcg
    13381 tcatgtgtgg tctggatgtc accaatcagg caatattaac ccctgactat ctcgctactt
    13441 tgccggagtt gaatcgcacc ggaaaaatgc tacacgccct gtttagccac taccgtagcg
    13501 gcagtatgca aagtggacta cgtatgcacg atctttgcgc tatcgcctgg ctggtgcgcc
    13561 cggaattgtt cactctcaaa ccgtgttttg tcgcggtaga aactcaaggc gaatttacct
    13621 caggcacgac ggtagttgat atcgacggtt gcctgggcaa accagccaat gtacaggtgg
    13681 cattggatct gaatgtaaaa ggcttccagc agtgggtagc tgaggtgctg gctctggtgc
    13741 cgtaacctgt cacatgttat cggcatgcag tcattcatcg actcatgcct ttcactgata
    13801 tccctccctg tttatcatta atttctaatt atcagcgttt ttggctggcg gcgtagcgat
    13861 gcgctggtta ctctgaaaac ggtctatgca aattaacaaa agagaatagc tatgcatgat
    13921 gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta tgggccgcca gttgattcag
    13981 gcggcgctgg cattagaagg cgtgcagctg ggcgcggcgc tggagcgcga aggatcttct
    14041 ttactgggca gcgacgccgg tgagctggcg ggtgcaggaa aaacaggcgt tactgtgcaa
    14101 agcagccttg atgcgataaa agatgatttt gatgtgttta tcgattttac ccgtccggaa
    14161 ggtacgctga accatctcgc tttttgtcgc cagcatggca aagggatggt gatcggcact
    14221 acggggtttg acgaagccgg taaacaggcg attcgtgacg ccgccgccga tattgcgatt
    14281 gtctttgccg ccaattttag cgttggcgtt aacgtcatgc ttaaactgct ggagaaagca
    14341 gccaaagtga tgggggacta caccgatatc gaaattattg aagcacatca tagacataaa
    14401 gttgatgcgc cgtcaggcac cgcactggca atgggagagg cgatcgccca cgctctggat
    14461 aaagatctga aagattgcgc ggtctacagt cgtgaaggcc ataccggtga acgtgtgcct
    14521 ggcaccattg gttttgccac cgtgcgtgca ggtgacatcg ttggcgaaca taccgcgatg
    14581 tttgccgata ttggcgagcg tctggagatc acccataagg catccagccg catgacattt
    14641 gctaacggtg cggtaagatc ggctttgtgg ttgagtggta aggaaagtgg tctttttgat
    14701 atgcgagatg tgcttgatct caataatctg taaccacaag atatttatgg tgatgcaaaa
//
LOCUS       DAZFRE010000110        13898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFRE010000110 DAZFRE010000000
VERSION     DAZFRE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.110"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            172..1230
                     /gene="citC"
                     /locus_tag="ACHKUI_004114"
     CDS             172..1230
                     /gene="citC"
                     /locus_tag="ACHKUI_004114"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6600198.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1245..1541
                     /gene="citD"
                     /locus_tag="ACHKUI_004115"
     CDS             1245..1541
                     /gene="citD"
                     /locus_tag="ACHKUI_004115"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6600199.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1538..2446
                     /gene="citE"
                     /locus_tag="ACHKUI_004116"
     CDS             1538..2446
                     /gene="citE"
                     /locus_tag="ACHKUI_004116"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6600200.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2457..3989
                     /gene="citF"
                     /locus_tag="ACHKUI_004117"
     CDS             2457..3989
                     /gene="citF"
                     /locus_tag="ACHKUI_004117"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6600201.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3993..4544
                     /gene="citX"
                     /locus_tag="ACHKUI_004118"
     CDS             3993..4544
                     /gene="citX"
                     /locus_tag="ACHKUI_004118"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6600202.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLISFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4519..5397
                     /gene="citG"
                     /locus_tag="ACHKUI_004119"
     CDS             4519..5397
                     /gene="citG"
                     /locus_tag="ACHKUI_004119"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6600203.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNISP
                     GGSADLLILTWFLAQI"
     gene            5448..6911
                     /gene="citT"
                     /locus_tag="ACHKUI_004120"
     CDS             5448..6911
                     /gene="citT"
                     /locus_tag="ACHKUI_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6600204.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7025..7831
                     /gene="rna"
                     /locus_tag="ACHKUI_004121"
     CDS             7025..7831
                     /gene="rna"
                     /locus_tag="ACHKUI_004121"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6600205.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYDDFDRYVLALSWQ
                     TGFCQSQYDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8061..8471
                     /gene="rnk"
                     /locus_tag="ACHKUI_004122"
     CDS             8061..8471
                     /gene="rnk"
                     /locus_tag="ACHKUI_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6600206.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8614..8703
                     /gene="yldA"
                     /locus_tag="ACHKUI_004123"
     CDS             8614..8703
                     /gene="yldA"
                     /locus_tag="ACHKUI_004123"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6600207.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8827..9255
                     /gene="uspG"
                     /locus_tag="ACHKUI_004124"
     CDS             8827..9255
                     /gene="uspG"
                     /locus_tag="ACHKUI_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6600208.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9376..10941)
                     /gene="ahpF"
                     /locus_tag="ACHKUI_004125"
     CDS             complement(9376..10941)
                     /gene="ahpF"
                     /locus_tag="ACHKUI_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6600209.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVHGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11112..11675)
                     /gene="ahpC"
                     /locus_tag="ACHKUI_004126"
     CDS             complement(11112..11675)
                     /gene="ahpC"
                     /locus_tag="ACHKUI_004126"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6600210.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12047..12793
                     /gene="dsbG"
                     /locus_tag="ACHKUI_004127"
     CDS             12047..12793
                     /gene="dsbG"
                     /locus_tag="ACHKUI_004127"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6600211.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
     gene            12998..>13898
                     /gene="ybdO"
                     /locus_tag="ACHKUI_004128"
     CDS             12998..>13898
                     /gene="ybdO"
                     /locus_tag="ACHKUI_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6600212.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQSLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
BASE COUNT         3388 a         3591 c         3426 g         3493 t
ORIGIN      
        1 aatcaaaatt tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata
       61 cctcgatcgc cccctttctc ccaagtgaaa ataaaaggtt atcaatttgc aacattgaac
      121 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      181 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      241 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      301 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      361 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      421 ctcgcctatg agcggcatag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      481 ctgttccgcc agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg
      541 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      601 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      661 ctgattcaac aggctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      721 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      781 ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc
      841 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      901 ttccgtcagt acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc
      961 ttttgtcgcg ttactgccca gtacaaccag gatatgcgct actggctgga aacaccgact
     1021 atctccgcac cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1081 atatccgctt cccgggtacg tcaactgctg gcaaaaaacg atctcacggc tatcgcgccg
     1141 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1201 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1261 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1321 cgcaggatat cgacctgcaa atcaacagca gcgttgagaa acagtttggc gatgcaattc
     1381 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1441 atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1501 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1561 acgtaaaact cgcacccgcc gcagtatgtt gtttgtgcct ggtgctaatg ccgcgatggt
     1621 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgttgc
     1681 attgcgtgaa aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgcttta
     1741 tcgcgatatt gaaaccattg tgcgtgtaaa cgcgctggat tccgaatggg gtgttaacga
     1801 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1861 tcaggatgtt ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga
     1921 acccggcagt accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcggt
     1981 agaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     2041 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg ctcgctgctc
     2101 cattttgcag gccgcgcgtt ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2161 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2221 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2281 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2341 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2401 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2461 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg
     2521 aatgcgatct tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc
     2581 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg
     2641 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2701 tggacgtcat cgcgaagatg ggctttaaaa acctgactct ggcctccagc tccctgagtg
     2761 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2821 ctggcctgcg cggtccactg gcggaagaaa tctcccgtgg tctgctggcg gaaccggtgc
     2881 aaatccactc tcacggcgga cgtgtgcatc tggtacagag cggcgaactg aatatcgatg
     2941 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tactccggta
     3001 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg
     3061 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3121 aggttgatct gatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg
     3181 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3241 ttgttaactc tggctacttc aaagaaggtt tctccatgca gaccggcacc ggcggcgctt
     3301 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3361 tcgccctcgg cggtattacc gcaacgatgg ttgatctgca cgaaaaaggt ttgatccgca
     3421 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3481 atcacatcga aatcagcgcc aaccagtacg ctaactgggg gtcgaaaggc gcagcggttg
     3541 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg
     3601 tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga
     3661 ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgacgctgg
     3721 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3781 acggcatcgc tgttaacccg gcacgtccgg agctggcaga acgtctgcag gaagcgggga
     3841 tcaaagtggt ttctattgag tggctgcgtg aacgtgcgcg tctgctgacc ggtgaaccac
     3901 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3961 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4021 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4081 acgcctggct caagcgccat cctgttccac tgatctcctt taccgtggtt gcgcctgggc
     4141 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4201 ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4261 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4321 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4381 gcgacattct ctcccgccgc gattattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4441 aaagcgcagc tgtttgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4501 tggaggcgct gctaaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4561 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaac
     4621 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4681 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4741 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tcaacggatt acgcccaatc
     4801 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaagga
     4861 agcatttttt ctttagggct actgtgtgcg gcaattggcc gtttgcttca gctcaaccag
     4921 ccggtaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     4981 cgcgaactgc gtaccaataa ttcacgactg acggcaggtc aacggttgta ccagcagctt
     5041 ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc
     5101 ttgccgcatt acctcactct gctggatcag gggttagatc ctgagctggc attgctcgat
     5161 accttgctcc tactgatggc taccaacggc gataccaacg ttgcatcgcg cggtggcgag
     5221 gggggcctgc gctggctaca gcgcgaggcg caaacattat taaacaatgg gggcatccga
     5281 acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatatc
     5341 agtccaggcg gcagtgccga cctgctgatt cttacctggt ttttagcaca gatttaatta
     5401 tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa
     5461 aagataatat atggaaatta ttggcgcccc tggtggtgat gggtgtcatg tttcttatcc
     5521 ctgtacctga cggtatgccg ccgcaggcgt ggcattactt tgcagtgttt gtggcaatga
     5581 ttgtcggcat gatcctcgag ccaattccgg caacggcgat cagttttatt gcggttacta
     5641 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5701 ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat
     5761 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggccgtc
     5821 gtattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga
     5881 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5941 gtacggtttt tccggtcatt aaaaacctgc cgccgctgtt taaatcattc ccgaacgatc
     6001 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     6061 gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca
     6121 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6181 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6241 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac
     6301 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6361 gtgaagtcat taatgctact gcggtaggtc tgctggcagt ttcgctgatg ctggcgctgc
     6421 acgttgtgcc gtggaaagac attactcgct ataacagcgc atggaacacg ctggtcaacc
     6481 tggcaacgct ggttgttatg gcgaatggtc tgacccgttc tggctttatc gactggttcg
     6541 ccaacacgat gagtacgcac ctggaaggct tctcaccaaa cgcaacggtg attgtactgg
     6601 ttctggtgtt ctactttgcc cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6661 tgctgccggt tattctggcc gtcggtaaag ggattccggg cgtaccaatg gaacaactgt
     6721 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccttat gcaaccggtc
     6781 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6841 caatcttcgg ggtgatttac atctctatgc tgctgttggt tggctggccg attctcgcca
     6901 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca
     6961 cctggcgggc ttttttatga tttaatagat aatattcagt gtatgtatga gttccacacc
     7021 cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc
     7081 ttctgccaac gccgtggcgt tgcaggcaaa acagtatgac gattttgatc gctatgtcct
     7141 ggccctctca tggcaaaccg gattttgtca gagtcaatac gaccgaaatc gtaacgaacg
     7201 agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttttga ccgtacatgg
     7261 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7321 gcgcttcggt tgcgctactc gcccaatccc gaatctgcca gaagcacgtg ccagccggat
     7381 gtgttcatcg ccggaaaccg ggttatcact ggaaacggca gctaaactaa gtgaagtcat
     7441 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7501 ctttggtttt gatccagacg catacttcgg cacgatggta cgcctgaatc aagaaattaa
     7561 agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga
     7621 ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7681 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7741 caacgctccg ctttctgcaa actcattctt gccacaacca cacccaggta actgtggcaa
     7801 aacctttgtg attgataaag caggttatta atcctctgat ttacaaaaca cgctaattcg
     7861 tctcccataa accatgacga ttaagattta tacataatgc gctacattga ggaattccct
     7921 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
     7981 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt
     8041 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
     8101 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
     8161 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
     8221 tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca
     8281 cgctggtgta tccggcaaaa atgaccgaca gcaatactca gctttccgtt atggctccgg
     8341 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
     8401 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
     8461 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
     8521 tcctctttcc gctcaaatat caaccagatt tatcattttg cgcttaagcg cagcattcta
     8581 atctgaactg gttattcatc tgcggaacct catatgagcg aagctctcgg tatcactgca
     8641 ttttatatat tcatagcagc aattattgtt gctgcagtgc tataccttga acaacgctgg
     8701 tagcgcctgg ttttatttat tttccagctc aatagcaaat tcagatcaaa tcagcctcgc
     8761 aaattataga ttcataacca ataatgtgct gttatgtata catgcaaaaa caaggagaga
     8821 atgcttatgt acaaaacaat catcatgcca gtggatgttt tcgaaatgga attgagcgac
     8881 aaagcgattc gccacgctga attcctcgcc caggatgacg gagttattca tctacttcac
     8941 gtactaccgg ggtcagccag cctgagcctg caccgttttg ccgctgatgt acgtcgtttt
     9001 gaagagcatc tgcaacatga agcagaagaa cgtttgcaaa cgatggtcag ccacttcacc
     9061 atcgatcctt cccgcattaa gcaacatgtc cgttttggta gtgtgcggga tgaagttaac
     9121 gagttagcga aagaacttga tgcagatgta gtagtgattg gttcgcgtaa cccatcaatt
     9181 tcaactcatc tgttgggttc taacgcctcg agcgtaatcc gccacgccaa tttgccggtg
     9241 ctggttgtgc gttaattgcc agaataagtc tcccgccctg cccggagcca tccctgcagg
     9301 gctttttaat gcaataaaaa agccgccagg tttgaccccg gcggctaagc aattacaggt
     9361 gaatcttact tcttcttatg cagttttggt gcgaatcagg tagtcaaaag cactcagaga
     9421 ggctttggca ccttcgccag tggcgatgat gatctgcttg tacggaaccg tcgtacagtc
     9481 acccgctgcg aacacgcctt tgacgttggt ttcgcatttc gcatcaatga taatctcgcc
     9541 catgcgatta cgttcgactg cgccttccag ccagttggtg ttcggcagca gaccaatctg
     9601 gacgaaaata ccggccagtt cgatgttgtg aatatcgccg ctgacacgat cgcgatattc
     9661 cagacctacg actttgctac cgtcgccttt cacttccgtg gtttgcgcat tcagaataat
     9721 gtcgacgttt ttcaggctgc gtagtttgtc ctgcagaacc tggtcggctt tcatttctgg
     9781 cgcaaattcc agcagcgtta cgtgctcaac gatacccgcc aggtcaattg ccgcttccac
     9841 gccggagtta ccgccgccga taaccgctac gcgtttgcct ttaaacagcg ggccgtcgca
     9901 gtgcgggcaa taggtcacgc ctttggtgcg atactgatct tcgccatgaa cgttcatgtt
     9961 gcgccatttt gcaccagtcg ccacgataat gctgcgtgct ttcagtaccg cgccagaagc
    10021 tgtttcaatc tgatgcaggc cgccttcaac cgctgccggg atcagtttgc tcgcgctctg
    10081 gctgtcgatc acatcaacgt cgtattcatc aacatgaact ttcagcgcac ctgccagttt
    10141 ctggccttcg gtcttcggta cagaaatgta gttttcgata tcaacggtat cgaggatctg
    10201 accaccaaaa cgttcgccca tcagaccggt acggatgcct ttacgtgcgg agtaaattgc
    10261 tgccgctgca cccgccgggc cggaaccaac gattaatacg tcataagcat cacgcttatt
    10321 cagctcttct gccgcacgtt tttccgcacc agtatcaatt ttggcaacga tttcagtcaa
    10381 cgtcatgcgg ccctgaccaa actctttccc gtttacgaac actgccggaa cgcccatcac
    10441 gttgcgatcg gtgatttcgt tctggaaggt gccgccgtca attgcagtgt gcttgatgcg
    10501 cgggttcagt acgctcatca ggttcagcgc ctgcaccacg tccgggcagt tgtggcaaga
    10561 gagcgagtaa taggtttcga attcaaaatc accgtcaata tggcgaatct gctccagcag
    10621 agactgcgct tctttcgacg gatgaccacc ggtccacaac aacgccagta ccagcgaggt
    10681 gaactcgtgg cccagcgggg agcctgcaaa acgtggtccc tggttggaac ctgggttggt
    10741 gatcaggaaa gacggcttac gcaccggcaa gctgttatct tctttaaagg tgactttgtc
    10801 tgacagttct gcgatttcag ccaacagttc cttgatttct gccgatttag cgctgtcatc
    10861 cagcgtggca attaactcaa caggcttggt caatttctca aggtaagcct tgagttgagt
    10921 tttcatattt gtgtcgagca tgtttatctc ctgggcttta aacatcatca tgcaagcggc
    10981 ctctgcggct agctggatgc agcttgcatc atggtgcggg gaagaaaatc gggtgccgca
    11041 gcacccgaag aattaggtga atttccgaag tatctcgcgc cggagttagt agcgcgaaag
    11101 acaaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    11161 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg tgctgctttg
    11221 attttacgca gcaggtcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    11281 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    11341 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    11401 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg ggtatcggta
    11461 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtaatggtc agcaacgtca
    11521 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    11581 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    11641 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    11701 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    11761 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    11821 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    11881 cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    11941 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    12001 ttatgccttt tactcgactt ttgcactgac tgaaaaggac aaattaatgt taaaaaagat
    12061 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctg ccccagtaaa
    12121 agcgattgaa aaacagggta ttacaatcat caaaacgttc gatgcccctg gaggaatgaa
    12181 aggttatctc ggtaagtatc aggatatggg cgtcaccatc tacctgactc cagatggtaa
    12241 gcacgctatc tctggttaca tgtacaacga aaaaggtgaa aacctgagta acacgcttat
    12301 cgaaaaagaa atttacgcac ccgccggacg tgaaatgtgg caacggatgg aacaatccca
    12361 ctggctcctc gacggtaaaa aagacgcgcc ggtcattgtc tacgtcttcg ccgatccgtt
    12421 ctgcccatat tgtaaacagt tctggcagca ggctcgtccg tgggtagatt ctggcaaagt
    12481 gcaattaaga acattgttgg ttggggttat caaaccagaa agcccggcga cagcagcggc
    12541 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggtggcaa
    12601 gcttaagcta agcgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagcgacaa
    12661 cgagaaactc atggacgatc tgggggcaaa tgtcacacca gctatctatt acatgagtaa
    12721 ggaaaatacc ctgcaacaag ccgtcggatt acccgatcag aaaacgctta atatcattat
    12781 gaggaataaa taaaggataa ttcatggatg gttgaaatat atccttgctt tttaaccatc
    12841 ctaaaaacgc atgaattgct cgtaacccac ctgacagcta tgctatttta gaaaatctta
    12901 atccaaagtt aagcagaaaa acataacaca tataaataca acaacattat tgatcaagtg
    12961 aatgatttat ggaagagaca atacaacgga gtatgttatg gctaatcttt atgatctaaa
    13021 aaaatttgac ctcaatttac tggtcatatt tgaatgtatt tatcaacatc tcagcattag
    13081 taaagcggca gaatcattat atataacgcc ctctgctgtt agtcagtcgt tacaacgttt
    13141 acgtgcgcaa tttaatgacc cgttattcat tcgctcaggg aaaggtatcg cgccgactac
    13201 aacagggtta aacctgcatc accatcttga gaaaaatctc agaggccttg aacaaacgat
    13261 caatatcgtt aataagtctg aactaaagaa aaattttatt atctacggtc ctcagcttat
    13321 ttcttgtact aataacaaca tgctaatccg ctgtcttaga caagatactt caatcgatat
    13381 agaatgccat gacatcctgc tttcagctga aaatgccgaa gaactgttgg ttcaacgcaa
    13441 aactgacctg gcaattacgc tgcaaccagt aattagtcgc tctgtcatct gcatgccgtt
    13501 acacactatt cgcaattccc ttatatgtag caacaaacat cctcgtatta ctgataccag
    13561 taactatgaa caaattatcg ccgaagaatt caccctgttg atatctaaat cagcagggat
    13621 tgacgaaatt caaatggaca tagatgaacg ttttatgaac cgtaaagttt catttcgcag
    13681 ttcttctttg atgactataa ttaatagtat ttctgttact gacttattag gcattgtgcc
    13741 caccgaactg tacgatttac atcgtgactt tttgaagtta aaagaaataa aactggaaca
    13801 atctcttcct gcggttaaac tttatatttc ctataacaag gcttcgttga acaaccttgt
    13861 cttctcccga ttcatagatc gtttaaatga tagcttct
//
LOCUS       DAZFRE010000111        13780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFRE010000111 DAZFRE010000000
VERSION     DAZFRE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.111"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(344..769)
                     /gene="arsC"
                     /locus_tag="ACHKUI_004129"
     CDS             complement(344..769)
                     /gene="arsC"
                     /locus_tag="ACHKUI_004129"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6600213.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1104..1187
                     /gene="dinQ"
                     /locus_tag="ACHKUI_004130"
     CDS             1104..1187
                     /gene="dinQ"
                     /locus_tag="ACHKUI_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6600214.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1241..2593)
                     /gene="gorA"
                     /locus_tag="ACHKUI_004131"
     CDS             complement(1241..2593)
                     /gene="gorA"
                     /locus_tag="ACHKUI_004131"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6600215.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2665..3507)
                     /gene="rlmJ"
                     /locus_tag="ACHKUI_004132"
     CDS             complement(2665..3507)
                     /gene="rlmJ"
                     /locus_tag="ACHKUI_004132"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6600216.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3710..5752
                     /gene="prlC"
                     /locus_tag="ACHKUI_004133"
     CDS             3710..5752
                     /gene="prlC"
                     /locus_tag="ACHKUI_004133"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6600217.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5760..6512
                     /gene="rsmJ"
                     /locus_tag="ACHKUI_004134"
     CDS             5760..6512
                     /gene="rsmJ"
                     /locus_tag="ACHKUI_004134"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6600218.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLAIKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6561..8030)
                     /gene="dtpB"
                     /locus_tag="ACHKUI_004135"
     CDS             complement(6561..8030)
                     /gene="dtpB"
                     /locus_tag="ACHKUI_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6600219.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8196..8257
                     /locus_tag="ACHKUI_004136"
                     /pseudo
     CDS             8196..8257
                     /locus_tag="ACHKUI_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8347..8781)
                     /gene="uspA"
                     /locus_tag="ACHKUI_004137"
     CDS             complement(8347..8781)
                     /gene="uspA"
                     /locus_tag="ACHKUI_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6600220.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9172..9507
                     /gene="uspB"
                     /locus_tag="ACHKUI_004138"
     CDS             9172..9507
                     /gene="uspB"
                     /locus_tag="ACHKUI_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6600221.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9578..11077)
                     /gene="pitA"
                     /locus_tag="ACHKUI_004139"
     CDS             complement(9578..11077)
                     /gene="pitA"
                     /locus_tag="ACHKUI_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6600222.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11309..12502
                     /gene="yhiN"
                     /locus_tag="ACHKUI_004140"
     CDS             11309..12502
                     /gene="yhiN"
                     /locus_tag="ACHKUI_004140"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6600223.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDLSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12589..13641)
                     /gene="yhiM"
                     /locus_tag="ACHKUI_004141"
     CDS             complement(12589..13641)
                     /gene="yhiM"
                     /locus_tag="ACHKUI_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO6600224.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3540 a         3475 c         3598 g         3167 t
ORIGIN      
        1 gttttagata tgtaataata aaataggtta acgtatgttg aaattattta ttgtgtaaac
       61 cactgataaa tcataatcca gtacatcttt aaactgctgc actgtatcgc tgataactaa
      121 gcagctcact gatacaccat gctggatgcg ataattggaa tctcacgcag ggagtactgt
      181 gtaaatagca aaattgcctg atgcgctacg actgtcaggc ctacaaataa catacaattc
      241 atcgaattat cataacgttg taggtcggat aagacgcgcc agcgtcgcat ccgacataaa
      301 taaagcgcat ttttctaaca acctgtgggg ggatatagcc gctttatttc aggcgattac
      361 ccgcctcatc aaccactttc tcgccatctt ccttggtgaa cgcgcctttt tgcgcatctg
      421 gcagaatttc cagcacgact tctgaagggc ggcacaggcg agttcccagc ggcgtcacca
      481 caatcgggcg attaataaga atcgggtgct gaagcataaa gtcgattaac tgatcgtgag
      541 taaatttatc ttctgcaagg cccagtttct catacggctc gacgttttta cgcagcaacg
      601 cgcgtacggt aatccccata tcggcaatga gttttaccag ctcatcgcgc gtcggtggtg
      661 tttccagata atagataatg gtcggttctg taccgctgtt gcggatcatc tccagcgtat
      721 tacgcgacgt accgcaggcc gggttgtgat aaatggtaat gttgctcata tcagtatctc
      781 attacgatgt gaaagagagg tgtagcgcca gcgccaccag cgttacaaac caaagcggac
      841 aagtcaaaaa cacatatggt ttaacgaata tgtaagtgta gagaaaggtg taggtgtgaa
      901 ttaagcgttg ggcattcatt tgaaaggagg tctgaatcag gacgtaaaaa aaccgacttc
      961 gcgtcggttt ttttactgtt cagccctgag ttggtggctc tggctggagt gagatagccc
     1021 acatctaaca atgagacatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc
     1081 actccatcgc atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa
     1141 ttgcgctgct ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta
     1201 gctaagggct aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact
     1261 cttctgccgc tgtcgggtga atggcaaccg tgttgtcgaa gtcttttttg gttgcaccca
     1321 tcttcagcgc caccgcgaag ccctgcaaca tttcgtccat accaaaacca atgccgtgaa
     1381 taccgacaat cttctcttcc ggtccaacgc ataccagttt catgcggcac ggctggcggt
     1441 gagtggtgac ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt
     1501 cgccgtactg ctcgcgcgcc tgcggctccg ttaaaccaac agtaccaatc ggcggatggc
     1561 tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca
     1621 ggcgttcaga gagacggcga cctgctgcaa cagccaccgg tgtcagttcc actgcacccg
     1681 tgttatcgcc caccgcgtaa ataccttcaa cgttggtgtt ttgatattta tcgacgacga
     1741 tataaccttt ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag
     1801 gctcgcgacc aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct
     1861 ccagcgtcag gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca
     1921 actgcgggcc ttcggcgttc atcacttcaa ccagcgtttc agagatcatc gggtcgaagc
     1981 tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtctt cgcaccgagg ccgttaatca
     2041 cgcccgccag ttcaacggcg atgtaacccg cgccaacaac cgctacacgc tctggcaaag
     2101 ccggaagggc gaagaagcca tcagaatcaa taccgtattc cacgcctgga atatccgggt
     2161 ggctcggacg accaccggtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttga
     2221 cttcaatggt tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt
     2281 taccgagcac gttctcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga
     2341 tcaacgtttc ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca
     2401 tatggatcgc ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa
     2461 catttacgca ggtgccgccc agctctttgg cttcaatcag tgcgcatttc tggccgtaca
     2521 tagccgcgcg gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat
     2581 agtgtttagt catgacatat tgtccttatc gttgattacc gcgattgtag cgcgagacgt
     2641 aataggtgcc agcaatggct gcaattactc tggcacgatc cagcttacgg tggcgtgccc
     2701 ggtgcctgcc ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc
     2761 cagcttccac ggcgggttaa tcacaatcat gccagaagcg gtcatgccac ggcgatcgct
     2821 gtctggcagc accgccagtt caatttgcag aattttgcga atgccggtcg cttccaggtc
     2881 gtggatcatg cgcttaattt gctggcgcag caccactgga taccacaatg cgtaagtgcc
     2941 agtggcgaaa cgtttgtaac cttctgctat cccgctgacc accgcctgat agtcggtttt
     3001 catttcatac ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagcttggc
     3061 cttaagctgc tggaaaccgt cggctttttc gacgcgcgca cggctgtctt tctgaaattc
     3121 agaacgcagc aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcctgttc
     3181 acgcagtagc tggcgcgcaa tcaacggcga accggggtaa taacgcaact gcccgctacg
     3241 gttgaagtgt tttaccacat tgatgtacgc ctccagctcc gcaggcaaat cgtcctgctg
     3301 ccagatacgg gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa
     3361 ctgataacgc ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt
     3421 cagcgactca atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc
     3481 gtgaaagctg tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc
     3541 cgtattcggg gcgcacaaaa gcctatcagg acagtatacc gaaaagaggc cgccgccgcg
     3601 aaagcgtaac gtttctcatt gaaattcact acacttaacc ccatgctaca aacattatgt
     3661 aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg actgcgctaa tgacgaatcc
     3721 gttactgact ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc
     3781 agccgtgact aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg
     3841 ggcaccgtac acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg
     3901 gcgtatcttc tccccggtca gccatctgaa ctcggtgaaa aatagcccgg aactgcgtga
     3961 agcgtacgaa caaaccctgc cgctgctgtc agaatacagc acctgggtag ggcaacatga
     4021 agggctgtat aaagcatatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac
     4081 ggcgcagaaa aaagcggttg ataacgcact gcgcgatttc gaactctctg gcatcggtct
     4141 gccgaaagag aaacagcagc gttacggcga aattgctacc cgtctttctg aactgggcaa
     4201 tcagtacagc aacaacgtcc tcgatgcgac gatgggctgg accaaactcg ttaccgacga
     4261 agcggagctg gcgggaatgc ctgaaagcgc gctggctgcg gcaaaagccc aggccgaagc
     4321 gaaagagctg gaaggttatt tgctgacgct ggatatccca agctacctgc cggtaatgac
     4381 ctactgcgac aaccaggctc tgcgcgaaga gatgtatcgc gcttatagca ctcgtgcttc
     4441 cgatcaaggt ccgaacgccg gtaagtggga taacagcaag gtgatggaag agatcctcgc
     4501 gctgcgtcac gaactggcgc aactgctggg ctttgaaaac tacgccttca aatcacttgc
     4561 cactaaaatg gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg
     4621 cgcacgcccg caaggcgaaa aagagctggc gcaactgcgc gccttcgcca aagccgaatt
     4681 tggcgtcgat gagttgcaac cgtgggatat cgcttactac agcgaaaaac agaaacagca
     4741 cctctacagc atcagcgatg aacaactgcg tccgtacttc ccggaaaaca aagcggttaa
     4801 cggcctgttt gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt
     4861 tgatgtctgg catccggatg tacgtttctt cgagctgtat gacgagaacg acgaactgcg
     4921 cggcagcttc tacctcgacc tgtatgcccg tgaaaacaaa cgcggcgggg cgtggatgga
     4981 tgactgcgta ggccagatgc gtaaagccga cggttcgctg caaaaaccgg tcgcgtatct
     5041 gacctgcaac ttcaaccgcc cggtaaatgg taaaccggcg ctgtttaccc atgacgaagt
     5101 gatcaccctg ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcattgaaac
     5161 cgctggtgtt tccgggatca gtggggtgcc gtgggatgcg gtcgaactgc cgagccagtt
     5221 tatggaaaac tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac
     5281 cggcgagccg ctgccgaagg agctgctgga taaaatgctg gcagcgaaaa attaccaggc
     5341 ggcgctgttt attctgcgcc agctggagtt cggcctgttc gatttccgcc tccatgcaga
     5401 gttccgtccg gatcaggggg cgaaaatcct cgaaactctg gcagaaatca agaaactggt
     5461 tgccgtggta ccgtctccgt catggggccg tttcccgcat gctttcagcc acatttttgc
     5521 cggtggttat gcggcaggtt actacagcta cctgtgggcc gatgtgctgg cggcagatgc
     5581 cttctcgcgt tttgaggaag agggcatttt caaccgtgaa actgggcaat cgttcctcga
     5641 caacattctg agccgtggcg gttcagaaga gccgatggag ctgttcaaac gcttccgtgg
     5701 tcgtgaacca cagctggatg cgatgctgga gcattacggc attaagggct gaacattcag
     5761 tgaaaatctg cttaattgat gaaacaggcg ccggagacgg cgccttatct gttctggcgg
     5821 cccgctgggg gctggaacac gatgaagaca acctgatggc gctggtgtta acgccggagc
     5881 atctggaact gcgcaagcgt gatgagccga aacttggcgg catctttgtt gattttgttg
     5941 gcggagcgat ggcgcaccga cgcaaattcg gcggcggacg tggtgaagcg gtggcaaaag
     6001 cagtgggcat taaaggcgat tatttgccgg atgtggtgga cgccaccgca ggattagggc
     6061 gcgatgcctt tgtgctggct tcggtcggct gccgcgtgcg gatgctggag cgcaatccgg
     6121 tggttgccgc gttgctcgac gatggtctgg cgcgtggtta tgcggatgcg gaaatcggcg
     6181 gctggttaca ggaacgtttg cagttgattc acgcctccag tctgacggcg ctgagcgata
     6241 tcaccccgcg tccgcaggta gtttatcttg acccaatgtt cccgcataag cagaaaagcg
     6301 cgctggtgaa aaaagagatg cgcgtgtttc agtcgctggt ggggccggat cttgatgccg
     6361 atggattgtt ggagcctgcg cggttgctgg caatcaaacg cgtggtggtg aagcgtccgg
     6421 actatgcgcc gccgctggcg aatgtcgcca cgccaaacgc ggtagtcact aaagggcatc
     6481 gctttgatat ttatgccgga acgccggtgt aaaagtaaag cggggtagcg ataacgctac
     6541 cccgctttct gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac
     6601 ggcaccatca atagcatcac tactgcaacg cccagcgtaa ccaggccaat cttaccaaac
     6661 acgttggtat agacgggcaa cgtctcaagt ggatcggtaa tgttgtctgg tactgcggta
     6721 aatgttgcca catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg
     6781 agaataaagc ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg
     6841 ctaataaaca gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat
     6901 gtcagcccct gggcatccgc aaaccacatt ccagccgctg cagccgtcaa aaagcccagt
     6961 gagcacataa acatgccgag agtaaacttc atcggcatcg agagatcttt gcctttgtta
     7021 cccagatgcg tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta
     7081 agcgcctgga agctgaccgg gttgatggaa aagccgagaa tttcatgatg cacgttgttg
     7141 atggcaaaga agttcagcga cgttggcatc tgggcgtaga gaatgtaaaa caccaccgct
     7201 tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat
     7261 gcctgacgaa agaagatgat ggtgacgacg atggagagaa caatcagaac cagattggcg
     7321 acttctacgt tgtgcatcag ccaggcgcag acgaagatca tcaccacgct gccaagtaat
     7381 acgtacaaca gtttactgaa gctcatcggc cggaagtcgg gttcagaacc aatgtctttc
     7441 accattccac gacaggcgat gtaaaccagt aatgcgataa ttaaccctgc accgcacagg
     7501 ttgtaggtga ctgaatagcc gaatttatca gcgatcacag gggccagcga taacgctatc
     7561 aacgagccga tattgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct
     7621 ttcggcgggt agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg
     7681 acagcgatag tccccagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc
     7741 atgaagtagc caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggttcccagc
     7801 aggtggtcgc caacatagcc accaatggaa atgaggccat agaccagcgc agcaaaagca
     7861 ccaaaagtga caaaagcctg ctcttgcgag aacccaagct gtttaacgaa gaaaaccgcc
     7921 agtacgccct gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg
     7981 aaaaatgggc gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac
     8041 cttccaatac catcctgaaa agtattaacg cgtaagggat aacgcgcgaa aaagtttaca
     8101 tctgtcagag attcttaata ttgatgcgga gaatagatta atacactatt acaacagaaa
     8161 ataaccagct gatcacacta atgatagaaa atataatggt gttaaatgtt gtattggcag
     8221 gggttttgat tgcgtacgca gaccgcaggc cagataaggc gtttacgctg catcaggtaa
     8281 caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtggag
     8341 ggaagattat tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt
     8401 gatcagctga cgtgcggaag acatcagttt gctccagaag tcctggtggt gaccacaaac
     8461 caccagatcc atatcgtatt tcttgattgc atcgaccagt acctggccca gatcgccgct
     8521 gccgctcagg gtttcagtga ttgggtagcc tgcattggtt gaaagctcgg tcagtgcatg
     8581 atgtgtctct tcagagatgc gtttctgcat atcacccaga ttcacatcga taagcccggt
     8641 gtataggtca gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg
     8701 agccatagag actgctttct ctaccagaac tttgctttcc ggggagaggt cgaccgcgat
     8761 gagaatgtgt ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag
     8821 ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac
     8881 ccagccgtaa agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt
     8941 caatccgcct tgcttactcg ttagataaac aaattttcga catgaatata ttgatagtgg
     9001 ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg
     9061 ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg
     9121 gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc
     9181 accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc
     9241 tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat
     9301 gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt
     9361 tggtatatct atgcccaacg ttatcgcgat catcacgatg atgagtttat tcgccgctgt
     9421 gagcgggtgc gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc
     9481 ctgattgcat tgatgatttg gcattaagca cttcagcaat aatgacgcat aaaaaaagcg
     9541 ggccagtaaa ctgacccgct cgttttggtg cgtacgatta caggaactgc aaggagagcc
     9601 agtacagccc gccggaaagc agtaccgcag ccggaagggt aaatacccag gccatcagaa
     9661 tactggttac ggttttacgc tgtaagccgc cgccatctac caccatcgtc cccgcgacag
     9721 aagaggagag tacgtgagta gtggaaaccg gcatcccggt ataacttgcc aggccgatag
     9781 acactgccgc cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct ttcttaccga
     9841 ttttctcacc gatagtcgtt gccacacgac gccagccaat catcgtaccg atacctaacg
     9901 ccagcgcgac cgccatgatg atccacaccg gcgcatactc gatggtgcta agcatgtcgg
     9961 acttcagttt cttcaacagg cgctgatcgt cagcactcac gccaggcatc ttcaccactt
    10021 tatcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt tgatcaagcg
    10081 acagcttgtc gtagctttcc acatcggtag tcaacatgcc tttcaggcgg ttgagcgcgt
    10141 taatggtatt cgacggatgg caatggaact ccgcgggttg cgttgcgcca gcttccggag
    10201 ccggtactaa ctgatcagca ccggtcgctt gtttcagcag cgcaggatgc tgctcaaagt
    10261 aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca gtggcattca
    10321 tgttcaccac gaagcctgct ggtgcgacac caatcaatac caacataacc agaccaatgc
    10381 ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc gatagcggaa aggatcagcg
    10441 caatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc tccgctgggg
    10501 tcaggtggat acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc aagaaaatca
    10561 gaccgccagc aaacaccagg ccgacaatag gggaaacgat cagagaaccg aaaatactta
    10621 atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac gcattggtta
    10681 aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt aaaccaaagt
    10741 accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc atggcaaggc
    10801 catgagacga tcccatatta agcagcagat ccgtcggcag catatgcaca atggcatagg
    10861 caacactcag accacccagc aaaacaccca aaaagttgaa caccgccgcc ataaccacgg
    10921 cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg gctgtgtcat
    10981 ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat aataacagcc
    11041 cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc attttgagga
    11101 catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa tatagacttt
    11161 ttttgatttg cctcctgtat ggattccact caaaaaataa ttatcttata taattcaggc
    11221 aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct ttacaattca
    11281 cgcccgtttt ttcttagagg agcgcaacgt ggaaaggttt gatgccatta ttataggcgc
    11341 tggtgcggca ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc gggttctgct
    11401 gatcgataat ggtaaaaaac cagggcgcaa aatcctcatg tctggcggtg ggcgctgcaa
    11461 ctttaccaac ctttatgtcg aaccgggcgc ttatctgagc cagaatccgc atttttgtaa
    11521 gtctgcactc gcgcgtttta cccagtggga tttcattgat ctggtcaata aacacggcat
    11581 cgcctggcac gagaaaacgt tagggcagct cttctgcgat gactccgcgc agcagattgt
    11641 cgacatgctg gtggatgagt gcgagaaggg caatgtggcc ttcagattgc gtagcgaagt
    11701 gctgagcgtg gcgaaggatg atacaggctt cacgcttgaa ctgaacggca tgactgtcgg
    11761 ttgcgaaaag ctggtcattg cgaccggtgg actgtcaatg ccggggctgg gcgcatcgcc
    11821 gtttggttat aagattgccg aacaattcgg cctcaacgtg ctgccgaccc gtgcgggtct
    11881 ggtgccattc actctgcata aaccgttgct tgaagagtta caggtgctgg cgggcgtggc
    11941 ggtgccttcc gtgattaccg ctgaaaacgg taccgttttc cgtgagaact tactcttcac
    12001 ccaccgcggc ttatctggac cggcggtgtt gcagatttcc agctactggc aaccgggtga
    12061 atttgtcagc atcaatctgc taccggatgt ggacctcgaa accttcctga atgagcagcg
    12121 taatgcacat ccgaatcaaa gcctgaaaaa cacgctggcg gttcatctgc cgaagcggtt
    12181 ggttgaacgg ttacaacaac tcgggcaaat cccggatctc tcgctaaaac agctcaacgt
    12241 gcgtgaccaa cagacactga ttagcacatt gactgactgg cgcgtacaac ccaacggcac
    12301 tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg aactctcttc
    12361 acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag tgatggacgt
    12421 caccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcat gggcttgtgc
    12481 gcaggatttg gtggcagaat aagaagcaaa atgtgctctc gataccagag ggattaacct
    12541 gaataaaacc agataaggcg tccaacgcct tatctgaagc cttttttatt attttttcgc
    12601 tgaacccgct tctaatatag aaacgatgga aaacagtgta aaacaaactg cacctaagcc
    12661 aaccagaact cgcgaaggaa taaagtaagc catatcggtt tgcgcaattt cagccaggaa
    12721 cgcagcaagg aacagacaaa acagacaggt aaataccgga atcatgggta ttctgttggc
    12781 taacgaacag gtgcgtcgcc ataccagcgc taacaaccac actttggaga aaatactgta
    12841 gcagatcatc cccaggcaaa tgagaataat gccgggggcc aggcgggcgc ttgcatctga
    12901 actgattaat acgtagatac caaaaataat ggtaagtgag ccaagcaaaa taacccaata
    12961 gcaccacagc caatgttctt tcactgagaa cgtattccgc gtttgatgga caatagtggc
    13021 gacaaggcca ataagacaag cgcaaattgc cgttaacccc attagtacgt gacctgcaac
    13081 ataatgtggt gtgatatctg cactacgtaa cagtatgact gaccaaatga aaccaaagag
    13141 agtaagcaga acgggaacgg caattaagca attaccgatt gttgaagaat aggcctgcaa
    13201 tggcgtaccg tcactcttac tgcccgaggc gtttttgggg atcaacagaa aatgtccgga
    13261 agatgccgct acggttgata cacaagcggc aatcatgcca acgccgaaaa taacatgacc
    13321 ggcgacaaat tcatctgcca tcacattatt gcttgccaac agtgaccatc cccaaatcat
    13381 agttgtagca gaaccggcat aaccaataac gggaaataac ctgttgtaga atttattcat
    13441 gccgtgagtt aactgagata taataataaa tgcggtagtg aacaatgcca gacatatcgc
    13501 agaaagagaa atcagaacat gccctgcaac gaaatattca ctttgccctg aaccggttaa
    13561 tacaaactca cctaatgcga tgcatattat acccatcagt aaaggaatta atttaaacaa
    13621 ccatccgata tatatattca ttttaaaccc catatatatt ttctgctaca cttcgaaaaa
    13681 catatgattc gtcttttcag ttagttaaat atgtctataa gatgccatca attaaaaaaa
    13741 gtaacttcac tataatcgga tttagcgtta tggggatcaa
//
LOCUS       DAZFRE010000112        13772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFRE010000112 DAZFRE010000000
VERSION     DAZFRE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.112"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            9..1142
                     /gene="tssD"
                     /locus_tag="ACHKUI_004142"
     CDS             9..1142
                     /gene="tssD"
                     /locus_tag="ACHKUI_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6600225.1"
                     /translation="MSDEIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDNNGLKTLNSKAVGRLLAAGGIYNGNIEGFRKTANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFNPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1139..1396
                     /locus_tag="ACHKUI_004143"
     CDS             1139..1396
                     /locus_tag="ACHKUI_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6600226.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1471..1716
                     /locus_tag="ACHKUI_004144"
     CDS             1471..1716
                     /locus_tag="ACHKUI_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6600227.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1885..4146)
                     /gene="metE"
                     /locus_tag="ACHKUI_004145"
     CDS             complement(1885..4146)
                     /gene="metE"
                     /locus_tag="ACHKUI_004145"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6600228.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4264..5217
                     /gene="metR"
                     /locus_tag="ACHKUI_004146"
     CDS             4264..5217
                     /gene="metR"
                     /locus_tag="ACHKUI_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6600229.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5105..6004)
                     /gene="bioP"
                     /locus_tag="ACHKUI_004147"
     CDS             complement(5105..6004)
                     /gene="bioP"
                     /locus_tag="ACHKUI_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6600230.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6080..6880)
                     /gene="yigL"
                     /locus_tag="ACHKUI_004148"
     CDS             complement(6080..6880)
                     /gene="yigL"
                     /locus_tag="ACHKUI_004148"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6600231.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6888..7910)
                     /gene="pldB"
                     /locus_tag="ACHKUI_004149"
     CDS             complement(6888..7910)
                     /gene="pldB"
                     /locus_tag="ACHKUI_004149"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6600232.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8021..8641
                     /gene="rhtB"
                     /locus_tag="ACHKUI_004150"
     CDS             8021..8641
                     /gene="rhtB"
                     /locus_tag="ACHKUI_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6600233.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8702..9322)
                     /gene="rhtC"
                     /locus_tag="ACHKUI_004151"
     CDS             complement(8702..9322)
                     /gene="rhtC"
                     /locus_tag="ACHKUI_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6600234.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9386..11215)
                     /gene="recQ"
                     /locus_tag="ACHKUI_004152"
     CDS             complement(9386..11215)
                     /gene="recQ"
                     /locus_tag="ACHKUI_004152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6600235.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11340..12209)
                     /gene="pldA"
                     /locus_tag="ACHKUI_004153"
     CDS             complement(11340..12209)
                     /gene="pldA"
                     /locus_tag="ACHKUI_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6600236.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12374..12841
                     /gene="yigI"
                     /locus_tag="ACHKUI_004154"
     CDS             12374..12841
                     /gene="yigI"
                     /locus_tag="ACHKUI_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6600237.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNKPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12893..>13772
                     /gene="rarD"
                     /locus_tag="ACHKUI_004155"
     CDS             12893..>13772
                     /gene="rarD"
                     /locus_tag="ACHKUI_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6600238.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQHR"
BASE COUNT         3469 a         3595 c         3416 g         3292 t
ORIGIN      
        1 catcaaagat gagtgatgaa atttacctga ccataaccgg agaacaacaa ggatgtattt
       61 cctctcgttg cggaacgagc gcatcaatag gtaatcgctg gcagattggc catgaagatg
      121 aaatcttcgc gttctcgcta tctaatagta ttacgaatac aggcaaaggg tcacaattac
      181 atggccttag tttctgtaag ttgattgaca aaagctcccc gttacttatt aacgctatta
      241 acaataacga gcagttgttc atggagtttg acttctatcg gatcaataga tttggtcgat
      301 gggagaaata ttacaacatt caactccgcg gcgcgctctt atcagcaatc aatcacctgt
      361 ttactgagaa caatctggat acagaaacca tcactgtcag ttatgaatat atactctcca
      421 ggcatctgat tgctaatact gaatttagtc atttggcatt tcccgataac tacaaccgtc
      481 tgtttatacc acggcaaaaa actaaagata ataatggcct caaaacactc aatagtaaag
      541 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcagga
      601 aaacagcaaa caagttggga ggagatgcac ctgctggtta cgatcaagtt atggataaca
      661 aagggttact catcgctggt gcttcaatag ttgctggagt tgttctggga agaatgagat
      721 tccctgattt ggaatcattt gagcatttcg gagccagagg agcagtatca ggaagaccat
      781 ttaatcccga ccttgccggt ggtccaatag aaaacctgac gatagatggc gtaacaatca
      841 atagggaagg tatcgctatt gttgaaaagc atatctccag atttgatcat gatccagcta
      901 atgatgtaat gatttcaagg ctaaaaaaaa tagcaaataa agaattgctt cctgaaaaat
      961 acgatctgaa ctactatact catgaatgcc gcgagtatca gcgttattgt aatttaggat
     1021 gggaaacagg tgaaccgaat ggtttagatg gatatgaatt atggaataat gcacatactg
     1081 caactctaga agactttaaa attaaagata ctgacttatt tcatccggat gcaaaaaaat
     1141 gacgattacg atagttgaat ttaacgtgct gaaagttatg gctgagaaag atattgactg
     1201 gagctggatg gtgctggatc gtactttagc tatacgaaat attccaggat ttggcaacgt
     1261 agcgaatatc gtaacaaagc tggttaatca tgggttggtt gatatcgtaa atggtgaggg
     1321 caactctaaa ccacgctaca gagtttctca atatggattg aatcttatca aagaacaaca
     1381 agacaacctc ttctgatcca gccattacag gagcatgatt tacattgtga tctttcaata
     1441 agatactcat caattttggt acagaatatc gtgatcacta aaaatgaact taacgtgctg
     1501 aacgttatga ctgagaaaga tataaactgg aactggatga atctcgatcg cacactatcc
     1561 ataaggcaaa taccaggatt cggtaatgtt gttaatattg ttaacaaatt agctaacgaa
     1621 ggtttggtta ttattgagga tagcgggcat aagtctatgc ctcactacca cgttagtgaa
     1681 aagggataca atctcgtgca acgtgagaat aaataacgta gcgttgatta ctgaacggaa
     1741 tcactcaaaa ctgtgttttg cgaaaaaaac gattcggtta tttctactcc tgaagatctg
     1801 cttcaatctg gcactcatgc aaaatggctt ccagtactgg aagccattat cgtactgcaa
     1861 tttacaatga ctcgttaata ctcatcatcc ccgacgcaaa ttctgcgccg cctgcaccat
     1921 gttcgccagt gccgcgcggg tttctggcca gccgcgcgtt ttcaggccac agtccgggtt
     1981 gacccacaga cgctctgccg gaatgcgttt tgccgctttc ttcagcaagg cttcaatcca
     2041 ttccacgctc ggtacatttg gcgagtgaat gtcatagacg ccaggaccga tttcatttgg
     2101 ataatcaaac tcttcaaacg actccagcaa ctccatgtcg gaacgcgagg tttcgatggt
     2161 gatgacgtct gcgtccaacg ccgcaatcga atccatgatg tcgttgaact cgcagtaaca
     2221 catgtgagtg tggatttgtg tgtcatcttt cgccacggcg gcgttgatac ggaaagcctc
     2281 cacgccccac tggagatagg catcccagtc gctgcgacgc agtggtaaac cttcgcgcaa
     2341 tgccggttcg tcaatctgaa tgatgccaat tccagcggct tccaggtccg cgacttcatc
     2401 acgcagcgcc agcgcaattt gtttggcgat ggtttcacgg ctgacatctt cacgcgggaa
     2461 cgaccagcag agaatagtca ccgggccggt caacatccct ttcaccggtt tatcagtcag
     2521 ggattgcgca tattttgccc actccacggt aatcggcgcc ggacgactaa cgtcaccaat
     2581 aacaatcggt ggcttcacgc agcgggaacc gtagctctgt acccaaccgt tttgcgtaaa
     2641 gacaaacccg tcgaggtgct cgccaaagta ttccaccatg tcattacgct cggcctcgcc
     2701 atgtaccagc acatccagtc ccaaacgttc ctgctcaaca atggcctgct tgatatgttc
     2761 cgcaatgccc gtacggtagt tattggcgtc gagattaccc tttttgaaat ccagacgcag
     2821 ggtacgaatc tccgtggttt gcgggaagga accaatcgtg gtggtcggcc acgcgggcag
     2881 tttaaaacgc gcacgctggg cttcagcacg cacttcatag acattcgcac gctgactgtc
     2941 ctgagcggtg atcgccgcca gacgcttttc tactgccgga ttatgtacac gagtagagtg
     3001 acgacgcgcc tgaatcggag cgctccactc tgccagagct gccgtatcac cactgttcag
     3061 cgcatcgcgc agcaatgcca gttcatgaca tttttgcagg gcgaaggcaa accagctttt
     3121 cacttctgca tcaagacgcg tttccacgct caggtcgatg gggctgtgca gcaacgagca
     3181 ggaagatgcc acccacaaat cgcgtttgcc gacaatgtcc ttaatttgcg catatttctc
     3241 ggtaagatcg gcgcgccaga cgttacgacc attgataaga cccgcagaca gcagccagtc
     3301 agaaggcaga cgcttgtgca gttcagcaac gtcatcttta ccatgtacga gatcgacatg
     3361 cagaccctga acaggcagcg cagtaatcgt gtcgaggttt ggcgttacgc cttcaaaata
     3421 ggtagtcagc agcagtttca cctgcccctg cagagcatcg taagcgggtt tgtacgcatc
     3481 cagccacgcc tgcggcagtt ccagaaccag cgcgggttcg tcgatctgta cccactcaat
     3541 gccgcgtttc gccagttctg ccagcacttg ctgataaacc ggcagaatgt cgttcagcag
     3601 gctaaggcgg tcaaactgtt cacctttcac tttacccagc cacaggtagg taatcggccc
     3661 cagcagcaca ggtttcacct tgtggcccag cgccagcgcc tcgtccactt cctccagcaa
     3721 ctgcgtccag gtcagtttga actgttggcc tttaacgaac tccggcacca tgtagtgata
     3781 gttggtgtta aaccatttgg tcatttccgc tgccgccgca ggttcgccag tcggtgcacg
     3841 tccacgacca atacggaaca gggtgtcgat atctaccgaa ccatctttgt tctggtgacg
     3901 cgccggaacg ttacccagca gcaggctggt ggtcaggaca tgatcgtacc aggcaaaatc
     3961 gcccaccggc agcaggtcga tacccgcttg cttttgttga tcccagtgac gagcacgcaa
     4021 ttcacgccct accgccagca gttcttcacg cgtggagttc cccgcccaat aactctcttg
     4081 cgcttttttc agctcgcgac gcaggccaac gcgagggaaa ccgagggtgt gattaagaat
     4141 tgtcattata tgccccttat ggaattttta gtatttagcc gtccagatgt ttacacatcc
     4201 ataattggcg gttactgtat attcctcaag cgcaaattat tcatgccgaa gtgaaggact
     4261 ttcatgatcg aagtaaaaca cctgaaaacg ctacaagcgt tgcggaactg cggctcactc
     4321 gcagccgctg cggcgacgtt gcatcaaacg caatccgccc tgtctcacca gtttagcgat
     4381 ctggaacaac gccttggctt ccggctattt gtgcgtaaga gccagccgct acgctttaca
     4441 ccgcagggag aaatcctgtt gcaactggca aaccaggtac tgccgcaaat tagccaggca
     4501 ctgcaagcct gcaatgaacc gcagcagacg cgtctgcgca ttgccattga gtgccatagc
     4561 tgtattcagt ggctgacgcc cgcgttagaa aatttccata agaactggcc gcaggtggag
     4621 atggatttta aatcaggcgt aacgtttgac ccgcagcctg cattgcagca gggcgagctg
     4681 gatctggtga tgacctccga tattctgccg cgcagtggcc tgcattattc gccgatgttc
     4741 gattatgaag tgcgtctggt gttggcacct gaccatccac tggcggcgaa aacgcgtatt
     4801 acaccggaag atctcgccag cgaaacgctg ttaatttatc cggtgcagcg tagccgactg
     4861 gatgtctggc gccattttct tcagccagca ggcgttagcc cgtcgctgaa aagcgtcgat
     4921 aacaccttgt tgttgattca gatggttgcc gcgcggatgg gtattgccgc cctgccgcac
     4981 tgggtggtgg agagttttga gcgccagggt ctggtcgtaa ccaaaaccct gggcgaagga
     5041 ttgtggagcc gactgtacgc cgccgtgcgc gatggcgagc agcgtcagcc gattacagaa
     5101 gcgtttattc gctcagcgcg caatcacgcc tgcgatcatc tgccgtttgt gaagagcgcg
     5161 gagcgaccca cttacgatgc acccacagtg aggccaggat caccagcgcg cctgtaataa
     5221 acgttggcca gtgcggctgt tggtgccaga tagccaggtt aaccaacagc cctgccggaa
     5281 cgtgcatatt attcataatg cccagcgttc cggcgtccac ctgcgtcgcg ccgtagttcc
     5341 acataaagta gccaataccg gaagccacca cgccaagaaa caccagaatg ccccattgca
     5401 gcgtggtttg cggcattttc tgcgcatttc ccagcaagaa ccatgcaatc actgccacca
     5461 ggaacgcgcc aagatagaac cacgcaaagg cgttatgctg cggcatcggg cgagtttcca
     5521 tcagacgttt gtaacccacc atgccgatgg caaaagtgat attggagagc tgcaccagca
     5581 acaagccagt ccagaaatgg tcggtgacct gatcatagcg aataatcccg gcaccaatca
     5641 ccgccaacaa ggcgctaaaa gcatagcccc agcgcagacg gcgcttactc atgatgtcat
     5701 aaatcagcgt gatgtagagc ggcgtcagta cggtgaacag caacagttcg gagaccgtca
     5761 ggtagagata ggcgcggaaa ctcagcatat acatcacacc gagctgcatc gcgcccacca
     5821 gcatatacag gccaaccgtt ttcaggctat tgccacgggt gcgcagaaac ggcagaaaaa
     5881 cgagtgccgc caggccaacg cgcaccagca ccgcaaaata gctatcgacg tgccccgcaa
     5941 ggtactcgcc ataaaagcta aaggagaagg cccacagaat cgtggtgatg ataagtagcg
     6001 ccacaatgga aaactctttg attaacgagt atccattgta gcgaagtatc aggttgacaa
     6061 ctgaccaaat aaagaacgat tacgacaaat agagtttacg cagataatgc ggcactgcgt
     6121 cttcggcgtt agtaccaatc acttccagct ctggatgcag atctttcagc cgttgatgcg
     6181 cgctgcccat aatgcagcct ttccccgcca tcgacagcat ttcggcatcg ttcattccat
     6241 cgccaaaggc aatacaatct tgcaggctgt agcccagttt cttcgccacc gcttccagcg
     6301 catggccttt tgaaacgccg cccgccatca cttccaggca ggttaaggta gagaaactga
     6361 cattgacgcg atcgccccag cgagcgttaa tcgcctgctc cagtggcaga agtttttcat
     6421 gggaatcgca ggtgaagaac actttgctga cgccttccgg ctccagcaat cccggctcat
     6481 acagcgcata attgaacacc gcttctttga aaaagcgcat ctcttccggg cgatggcgat
     6541 tcataaacca ttcatcatcg cgataaacgt tagtaatgat ctccgggttg tcgttgacca
     6601 cgccaaacag gtcactggca atgtcgcgat ccaggttatg agcaaaaatc aggttgccat
     6661 ccagatcgtg aacgcgcgcg ccattggagg taatcatgta agacttaatc tccagattat
     6721 cgcgaatttg ccccacatca acgtggtgac gaccggtcgc aaacacaaag ttgatgccgc
     6781 gcgcggtgag cagcttcaga gtttctttgg cgtaagggga taacgtatgg tcgggagaaa
     6841 gtaacgtgcc atctaaatca gacgcaacaa cctggtacat aagaaattta aacctctgta
     6901 gagcggtttc cgctgggtga gttatgcctg ttgaaaaaat caacgatagc gtggagcgcg
     6961 actgagcgca ttgcgtcctt ttcaaaaagg atctcatggt acgcaccttt aattaccaac
     7021 ggccgtcctc cttcgacagg atggcccgca gcggtgcgta gttcacaaaa acggtcgtgc
     7081 atgcggttat ccaccacgcg ttcctcttca gcttgtaaga gaagcgttgg cgtggcgtca
     7141 tcacccgcac cggctaacac ctgttcgcca gccagaatac tttcgcgtac ccaatggtag
     7201 gtcggcccac cgacgcgaat tgttgggtca tcagcataga aacgtaagtt acgtcgatat
     7261 cgctgtctgc tgtgggtcag tacgttgata gcaaacggca acgcgcgcca gcgcccggtg
     7321 cctattgcat agccatcacg gaaacgtgga tgcgcttcgg cccaattgag gatctgccgt
     7381 gccataaatg acggcatacg aatcacgatc ccaaacattg gcgcagttag cgcaatggcg
     7441 tcacatacac ctggatggcg ttgcagaaat aatgtggaga tcgcaccgcc catcgaatgt
     7501 gccagtatat agcgtttacg ccacggaccg ggctgaacct cctgttgcca gaatgccgcc
     7561 agatcatcaa cataatcatt aaagcgatta acatgcccga gatgtggatc gcctaacagg
     7621 cgaccggaac gtccctgccc gcgatggtcg atgattaaaa catcaaaccc caaatggaac
     7681 aggtcgtagg ccagttccgc atattttacg tagctctcaa tacgccccgg acagatgact
     7741 accacccggt catggtgctg tgcgcgaaaa cggacaaagc gcaccggaat gtcatccaca
     7801 ccagtaaact ctgcttcatc acgctgacgc cagaaatcag tcagcggtcc catggtaaaa
     7861 gcagcaaacg cgttttctct tgtttcccag tctttttgct gctgaaacat cgggtaatct
     7921 gcctcttaaa ccacgtaaaa tcgttttttt aagcgtgcct gacacaacgc tgcgacagta
     7981 gcgtattgtg gcacaaaaat agacacaccg ggagttcatc atgaccttag aatggtggtt
     8041 tgcctacctg ctgacatcga tcattttaag cctgtcgcca ggctctggcg caatcaacac
     8101 catgaccacc tcgctcaacc acggttatcg cggcgcggtg gcgtctattg ctgggcttca
     8161 gaccggactg gcgattcata ttgtgctggt tggcgtgggg ttggggacgc tattttcccg
     8221 ctcagtgatt gcgtttgaag tgttgaagtg ggcaggcgca gcttacttga tttggctggg
     8281 aatacagcaa tggcgcgccg ctggcgcaat tgatcttaaa tcgctggcct ccactcaatc
     8341 gcgtcgacat ttgttccagc gcgcagtttt tgtcaatctc accaatccca aaagtattgt
     8401 gtttctggcg gcgctatttc cgcaattcat catgccgcaa cagccgcagc tgatgcagta
     8461 tatcgtgctc ggcgtcacca ctattgtggt cgatattatt gtgatgatcg gttacgccac
     8521 ccttgctcaa cggattgctc tatggattaa aggaccaaag cagatgaagg cgctgaataa
     8581 gattttcggc tcgttattta tgctggtggg agcgctgtta gcatcggcga ggcatgcgtg
     8641 aaaataatgt cggatgcggc gtaaacgcct tatccgactt actctgatga cgcgtctggc
     8701 attaacgcga aataatcaaa tgaatgccaa atccggcaaa taacgccccg gcaaaaccat
     8761 caatccactt cgccagacgt tgataaccac ggcgcatttg cggcagagca aacaggctgg
     8821 caacgacggt aaaccacgcc agtgtttcga caataatcaa cgcgaaaatg ccccagcgtt
     8881 cggtggtgcc gacattatca ccgacaaaca gcgagaacac cgaaccaaag tagataatcg
     8941 ctttagggtt cgcgagattg gtcagtaagc ccttaagaaa actgcgcccg cttttcgcca
     9001 gttcgacttg tggcgcaggg gcagaaacaa cctctttttt cagtgcacca cgtagcatct
     9061 ggtaacccat ccagcagaga tacaggccac cgcccaccat aatcagcgta tgcagccagg
     9121 ccattttttc gataatcaaa tgtaggccaa gcaacgcaat cccagcccaa accattacgc
     9181 cgcaggtaat gcccagcacg cccatcatcg cttctttacg ggaacgacta acagcggtct
     9241 gagagacaaa aaagaaatcg ggaccggggc tcataagcgc cacaatgtgc accatggcga
     9301 cggtgaggaa taacatcaac atacattaga ctcgcggggg aaacgtttac ggagtcttca
     9361 tcctggcact tttttatgct gctgactact cttcgtcgtc accatcgaca tgcgcacgaa
     9421 tcagcgccat aaacggtttg ccaaagcgtt ccagcttgcg cataccaacg ccgttaacgc
     9481 tgagcatttc gctggcggtg atcggcatct gctccgccat ctcaatcaag gttgcgtcgt
     9541 taaacaccac gtacggcggg acattgcttt catcggcaat cgatttacgc agtttgcgta
     9601 atttggcgaa cagtttgcga tcatagttgc cgccgaacga tttctgcatc gctttcggtt
     9661 tgagcgccac gatacgcggc acggcaagtt gcaaagagga ttcgccgcgc agcaccggac
     9721 gcgcagcctc tgtcagttgt agggcagaat gctgggcaat attttgcgtc accaggccga
     9781 ggtgaatcag ctggcggatc acgctcaccc aatgttcatg gcttttatca cggcccatgc
     9841 catagacttt cagtttgtca tgaccatagt cgcggatacg ctggttatta gcaccgcgaa
     9901 tcacttccac cacataaccc atcccaaacc gctgattcac acgaccaatg gtggaaaggg
     9961 caatctgcgc atcggttgaa ccatcgtact gttttggcgg atcgaggcag atatcgcagt
    10021 tcccgcacgg ctcctgacgc ccttcgccaa aatagttcag caataccaaa cgacggcaag
    10081 tttgcgcttc agcaaacgcg cccatcgcat tgagtttatg acgttcgata tcctgcaact
    10141 gcccctgcgg cttctcttcc agacaacggc gcagccacgc catatcagcc ggatcgtaaa
    10201 acagcatcgc ttccgcgggc aggccatcac gcccggcgcg tccagtttcc tgataatagg
    10261 attcgatatt gcgcggaata tcaaaatgca ccacgaagcg aacgttaggc ttgttgatgc
    10321 ccatgccgaa cgccaccgtc gccaccacaa tttgcaggtc atcgcgctgg aatttttcct
    10381 gcacatcagc gcgaacatta ttttccagcc cggcatgata ggccgccgcg ctaattccct
    10441 tgctttgcag gcgcgcagcg gtgtcttcta ctttcgcacg actattacag taaataatcc
    10501 ccgacttacc gcgttgttcc tgtacataac gcatcaactg atcgagcggt ttgaatttct
    10561 ccatcagcat gtagcgaata ttcggacggt caaaactgct gatttgaatc agcggatcgt
    10621 tcagccccag caggcgcacg atatcctggc gcgtggtgtc atcggctgtg gcagtcagcg
    10681 ccataaacgg gagcgtcgga aaacgctgac gcagctgacc gagggcagcg tattccgggc
    10741 ggaaatcgtg accccattgg gagatacagt gcgcttcatc aacggctaat aacaccggat
    10801 tccagtgcgc cagatgctca agaaagttat ccagcatcag gcgttccggc gcgatataaa
    10861 gcaggctgat ttgcccggtg cgacagcctg tcatcacttc aagctgctgc tcgcgggttt
    10921 gcgtcgagtt aaggcacgcc gccgccacgc cgttggcttg cagttgatct acctgatctt
    10981 tcatcaacga aatcagcggt gagacaacca cggtaaggcc gtttagcaat aaggcaggga
    11041 tttgatagca aagggatttt ccgccaccag tgggcatgac gaccaggcaa tcgcgaccgg
    11101 aaagtacggt gtcgataatc tcttcctggc cggggcgaaa ctgttggtag ccaaaggttt
    11161 cttgtaaaac ctgtttagct ccggactcca gattcaacac ttccgcctgc gccacattca
    11221 ccccgattgc ctgaaataaa aacaggcgct attttcagcg cctgcgcgag aaactgcaat
    11281 gcttttagtc tctgagaggg taatcctgga tgctggattc cagatttgcc aaccattcat
    11341 caaaacaggt cgtttagcat aactcccaca ccaacgcggg tctggttgaa gttatagtcg
    11401 atgagcgatt cgccgtaacc actgtaaacc tgagtataaa ggcgcacatg tttggtgatc
    11461 gggtagctta agcctaactc cgcgccgccg taaccggtgt tccagttgta ctgccctttc
    11521 gcgctcagta ccgcatcacc gaggtgatag ccgattttaa gctggtagta acccatatat
    11581 ttggtgatgt ccgggttatc gtccgtattc cccaccacat accacggctt cacctctacc
    11641 agccagttac cgttttctgc catcaggcgg gtataaaggc ggttccagct gcgggaagtc
    11701 gggtctgaac gcccgtttga gtcgtggttg tagcccattt ccacatcgcg cagcgtccag
    11761 ccagcaaagt tgtaatccgt ggcaaaaccg aggaacaatt gcggttcgta gttagtttca
    11821 cgaaacggtg atgactcatc gctattagaa agctgccacc aggatttctg tgtgtaggag
    11881 gcgcccagta cagagtttgg ccccaaaatc ccacgccaca gcggaaacgc caggctcaac
    11941 tggaacttta cttcatcctt acgcgcgttt tccgcccagt cgtaactggc aatcgcttct
    12001 ttattcagat cgctggtttg ggtgtagatg atgtagttgg tgtcataagg ataaagggtg
    12061 aacggattgt catgctcctg cagcatattg gcgataatac tgccacgcac cgctggcgcg
    12121 tcatgcacct ctttcaccgt tgcctcttgt gcatatactg ccataggcaa cataaacacc
    12181 ggcaacaacc agccctgcag agtccgcatc gctcgtgttc tccgtaacga attattgata
    12241 aggtgaatat ttttctgcgg gctattttac atacttaact ccatagccgt tagttatccc
    12301 ttcctaaagt agaaaccaac aataaataag cataaaatca acatttcatt aactaatgaa
    12361 gtgtaaggat gttatgtctg ccgtactgac cgctgaacaa gccctcaaat tagtgggtga
    12421 gatgtttgtt tatcacatgc catttaaccg cgcattgggg atggaactgg agcgttacga
    12481 aaaagagttc gcacagctgg cctttaaaaa taagccgatg atggtgggca actgggcgca
    12541 aagcatttta cacggcgggg tcattgcgtc ggcgctggat gtcgccgcag gtctggtgtg
    12601 cgtgggaagt accttaaccc gccacgaaac catcagtgaa gatgaactac gccagcggct
    12661 atcgcggatg gggaccattg atcttcgcgt cgattatctg cgcccaggca gaggcgagcg
    12721 ttttactgct accagtagcc tgttacgtgc aggcaataaa gtcgccgtcg cccgcgttga
    12781 attacacaat gaagaacagc tttatattgc cagtgccacc gccacctata tggtaggttg
    12841 aagacataaa agcaggtaaa ctgctgttac caattaaaaa tgagtattcc cgatggatgc
    12901 aaaacaaacg cggcagggcg tattactcgc tcttgccgct tattttattt ggggtattgc
    12961 gccagcgtac ttcaaactga tttactacgt gcccgccgat gagatcctga cgcatcgcgt
    13021 gatctggtcg tttttcttta tggtggtgct gatgagcatt tgccgccagt ggtcctattt
    13081 aaaaacgctg attcagacgc cacagaaaat ttttatgctg gcagtctctg ccgtgctgat
    13141 tggcggcaac tggctactgt ttatctgggc ggtgaacaat caccatatgc tggaagcgag
    13201 ccttggttac tttattaacc cgctggtgaa cattgtgctg gggatgattt tcctcggcga
    13261 gcgattccgc cggatgcaat ggctggcggt gattctggcg atatgtggag tgctggttca
    13321 gttgtggact tttggttcac tgccgatcat cgcgctgggg ctggcgttta gttttgcttt
    13381 ctacggcctg gtgcgcaaga agattgctgt tgaagcgcaa accggcatgt taatcgaaac
    13441 tatgtggttg ctgcccgtgg cggcgattta cctgtttgct attgccgaca gctcaaccag
    13501 tcatatgggg caaaacccga tgtcgctgaa tttactgctg atcgccgccg gtattgtcac
    13561 caccgtgcca ctgttgtgtt ttactgcagc tgcgacacgc ctgcgtctct caacgttagg
    13621 ctttttccag tacattggtc cgacgctgat gttcctgctg gctgtgacgt tttacggtga
    13681 aaaaccgggt gccgataaga tggtaacttt cgcctttatt tgggtggcgc tggcaatttt
    13741 tgtgatggat gcgatttata cgcagcatag aa
//
LOCUS       DAZFRE010000113        13646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFRE010000113 DAZFRE010000000
VERSION     DAZFRE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.113"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            262..810
                     /gene="fimA"
                     /locus_tag="ACHKUI_004156"
     CDS             262..810
                     /gene="fimA"
                     /locus_tag="ACHKUI_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6600239.1"
                     /translation="MKIKTLAIVVLSALSLSSTVALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            875..1414
                     /gene="fimI"
                     /locus_tag="ACHKUI_004157"
     CDS             875..1414
                     /gene="fimI"
                     /locus_tag="ACHKUI_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600240.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRSPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            1451..2176
                     /gene="fimC"
                     /locus_tag="ACHKUI_004158"
     CDS             1451..2176
                     /gene="fimC"
                     /locus_tag="ACHKUI_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6600241.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            2243..4879
                     /gene="fimD"
                     /locus_tag="ACHKUI_004159"
     CDS             2243..4879
                     /gene="fimD"
                     /locus_tag="ACHKUI_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO6600242.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            4889..5419
                     /gene="fimF"
                     /locus_tag="ACHKUI_004160"
     CDS             4889..5419
                     /gene="fimF"
                     /locus_tag="ACHKUI_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6600243.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            5492..5935
                     /locus_tag="ACHKUI_004161"
     CDS             5492..5935
                     /locus_tag="ACHKUI_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600244.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            5955..6857
                     /gene="fimH"
                     /locus_tag="ACHKUI_004162"
     CDS             5955..6857
                     /gene="fimH"
                     /locus_tag="ACHKUI_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6600245.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7100..8443)
                     /gene="gntP"
                     /locus_tag="ACHKUI_004163"
     CDS             complement(7100..8443)
                     /gene="gntP"
                     /locus_tag="ACHKUI_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO6600246.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            8783..9967
                     /gene="uxuA"
                     /locus_tag="ACHKUI_004164"
     CDS             8783..9967
                     /gene="uxuA"
                     /locus_tag="ACHKUI_004164"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6600247.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10048..11508
                     /gene="uxuB"
                     /locus_tag="ACHKUI_004165"
     CDS             10048..11508
                     /gene="uxuB"
                     /locus_tag="ACHKUI_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6600248.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            11723..12496
                     /gene="uxuR"
                     /locus_tag="ACHKUI_004166"
     CDS             11723..12496
                     /gene="uxuR"
                     /locus_tag="ACHKUI_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6600249.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(12637..13467)
                     /gene="yjiC"
                     /locus_tag="ACHKUI_004167"
     CDS             complement(12637..13467)
                     /gene="yjiC"
                     /locus_tag="ACHKUI_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFO6600250.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
BASE COUNT         3520 a         3283 c         3514 g         3329 t
ORIGIN      
        1 atcttcctca ttcttctctc caaaaaccac ctcatgcaat ataaacatct ataaataaag
       61 ataacaatag aatattaagc caacaaataa actgaaaaag tttgtccgcg atgctttcct
      121 ctatgagtca aaatggcccc aaatgtttca tcttttgggg gaaaactgtg cagtgttggc
      181 agtcaaactc gttgacaaaa caaagtgtac agaacgactg cccatgtcga tttagaaata
      241 gttttttgaa aggaaagcag catgaaaatt aaaactctgg caatcgttgt tctgtcggct
      301 ctgtccctca gttctacagt ggctctggcc gctgccacga cggttaatgg tgggaccgtt
      361 cactttaaag gggaagttgt taacgccgct tgcgcagttg atgcaggctc tgttgatcaa
      421 accgttcagt taggacaggt tcgtaccgca tcgctggcac aggaaggagc aaccagttct
      481 gctgtcggtt ttaacattca gctgaatgat tgcgatacca atgttgcatc taaagccgct
      541 gttgcctttt taggtacggc gattgatgcg ggtcatacca acgttctggc tctgcagagt
      601 tcagctgcgg gtagcgcaac aaacgttggt gtgcagatcc tggacagaac gggtgctgcg
      661 ctgacgctgg atggtgcgac atttagttca gaaacaaccc tgaataacgg aaccaatacc
      721 attccgttcc aggcgcgtta ttttgcaacc ggggccgcaa ccccgggtgc tgctaatgcg
      781 gatgcgacct tcaaggttca gtatcaataa cctacccagg ttcagggacg tcattacggg
      841 cagggatgcc cacccttgtg cgataaaaaa aacgatgaaa aggaagagat tatttctatt
      901 agcgtcgttg ctgccaatgt ttgctctggc cggaaataaa tggaatacca cgttgcccgg
      961 cggaaatatg caatttcagg gcgtcattat tgcggaaact tgccggattg aagccggtga
     1021 taaacaaatg acggtcaata tggggcaaat cagcagtaac cggtttcatg cggttgggga
     1081 agatagcgca ccggtgcctt ttgttattca tttacgggaa tgtagcacgg tggtgagtga
     1141 acgtgtaggt gtggcgtttc acggtgtcgc ggatggtaaa aatccggatg tgctttccgt
     1201 gggagagggg ccagggatag ccaccaatat tggcgtagcg ttgtttgatg atgaaggaaa
     1261 cctcgtaccg attaatcgtt ctccagcaaa ctggaaacgg ctttattcag gctctacttc
     1321 gctacatttc atcgccaaat atcgtgctac cgggcgtcgg gttactggcg gcatcgccaa
     1381 tgcccaggcc tggttctctt taacctatca gtaattgttc agcagataat gtgataacag
     1441 gaacaggaca gtgagtaata aaaacgtcaa tgtaaggaaa tcgcaggaaa taacattctg
     1501 cttgctggca ggtatcctga tgttcatggc aatgatggtt gccggacgcg ctgaagcggg
     1561 agtggcctta ggtgcgactc gcgtaattta tccggcaggg caaaaacaag tgcaacttgc
     1621 cgtgacaaat aatgatgaaa atagtaccta tttaattcaa tcatgggtgg aaaatgccga
     1681 tggtgtaaag gatggtcgtt ttatcgtgac gcctcctctg tttgcgatga agggaaaaaa
     1741 agagaatacc ttacgtattc ttgatgcaac aaataaccaa ttgccacagg accgggaaag
     1801 tttattctgg atgaacgtta aagcgattcc gtcaatggat aaatcaaaat tgactgagaa
     1861 tacgctacag ctcgcaatta tcagccgcat taaactgtac tatcgcccgg ctaaattagc
     1921 gttgccaccc gatcaggccg cagaaaaatt aagatttcgt cgtagcgcga attctctgac
     1981 gctgattaac ccgacaccct attacctgac ggtaacagag ttgaatgccg gaacccgggt
     2041 tcttgaaaat gcattggtgc ctccaatggg cgaaagcacg gttaaattgc cttctgatgc
     2101 aggaagcaat attacttacc gaacaataaa tgattatggc gcacttaccc ccaaaatgac
     2161 gggcgtaatg gaataacgca gggggaattt ttcgcctgaa taaaaagaat tgactgccgg
     2221 gtgattttaa gccggaggaa taatgtcata tctgaattta agactttacc agcgaaacac
     2281 acaatgcttg catattcgta agcatcgttt ggctggtttt tttgtccgac tcgttgtcgc
     2341 ctgtgctttt gccgcacagg cacctttgtc atctgccgac ctctatttta atccgcgctt
     2401 tttagcggat gatccccagg ctgtggccga tttatcgcgt tttgaaaatg ggcaagaatt
     2461 accgccaggg acgtatcgcg tcgatatcta tttgaataat ggttatatgg caacgcgtga
     2521 tgtcacattt aatacgggcg acagtgaaca agggattgtt ccctgcctga cacgcgcgca
     2581 actcgccagt atggggctga atacggcttc tgtcgccggt atgaatctgc tggcggatga
     2641 tgcctgtgtg ccattaacca caatggtcca ggacgctact gcgcatctgg atgttggtca
     2701 gcagcgactg aacctgacga tccctcaggc atttatgagt aatcgcgcgc gtggttatat
     2761 tcctcctgag ttatgggatc ccggtattaa tgccggattg ctcaattata atttcagcgg
     2821 aaatagtgta cagaatcgga ttgggggtaa cagccattat gcatatttaa acctacagag
     2881 tgggttaaat attggtgcgt ggcgtttacg cgacaatacc acctggagtt ataacagtag
     2941 cgacagatca tcaggtagca aaaataaatg gcagcatatc aatacctggc ttgagcgaga
     3001 cataataccg ttacgttccc ggctgacgct gggtgatggt tatactcagg gcgatatttt
     3061 cgatggtatt aactttcgcg gcgcacaatt ggcctcagat gacaatatgt tacccgatag
     3121 tcaaagagga tttgccccgg tgatccacgg tattgctcgt ggtactgcac aggtcactat
     3181 taaacaaaat gggtatgaca tttataatag tacggtgcca ccggggcctt ttaccatcaa
     3241 cgatatctat gccgcaggta atagtggtga cttgcaggta acgatcaaag aggctgacgg
     3301 cagcacgcag atttttaccg taccctattc gtcagtcccg cttttgcaac gtgaagggca
     3361 tactcgttat tccattacgg caggagaata ccgtagtgga aatgcgcagc aggaaaaacc
     3421 ccgctttttc cagagtacat tactccacgg ccttccggct ggctggacaa tatatggtgg
     3481 aacgcaactg gcggatcgtt atcgtgcttt taatttcggt atcgggaaaa acatgggggc
     3541 actgggcgct ctgtctgtgg atatgacgca ggctaattcc acacttcccg atgacagtca
     3601 gcatgacgga caatcggtgc gttttctcta taacaaatcg ctcaatgaat caggcacgaa
     3661 tattcagtta gtgggttacc gttattcgac cagcggatat tttaatttcg ctgatacaac
     3721 atacagtcga atgaatggct acaacatcga aacacaggac ggagttattc aggttaagcc
     3781 gaaattcacc gactattaca acctcgctta taacaaacgc gggaaattac aactcaccgt
     3841 tactcagcaa ctcgggcgca catcaacact gtatttgagt ggtagccatc aaacttattg
     3901 gggaacgagt aatgtcgatg agcaattcca ggctggatta aatactgcgt tcgaagatat
     3961 caactggacg ctcagctata gcctgacgaa aaacgcctgg caaaaaggac gggatcagat
     4021 gttagcgctt aacgtcaata ttcctttcag ccactggctg cgttctgaca gtaaatctca
     4081 gtggcgacat gccagtgcca gctacagcat gtcacacgat ctcaacggtc ggatgaccaa
     4141 tctggctggt gtatacggta cgttgctgga agacaacaac ctcagctata gcgtgcaaac
     4201 cggctatgcc gggggaggcg atggaaatag cggaagtaca ggctacgcca cgctgaatta
     4261 tcgcggtggt tacggcaatg ccaatatcgg ttacagccat agcgatgata ttaagcagct
     4321 ctattacgga gtcagcggtg gggtactggc tcatgccaat ggcgtaacgc tggggcagcc
     4381 gttaaacgat acggtggtgc ttgttaaagc gcctggcgca aaagatgcaa aagtcgaaaa
     4441 ccagacgggg gtgcgtaccg actggcgtgg ttatgccgtg ctgccttatg ccactgaata
     4501 tcgggaaaat agagtggcgc tggataccaa taccctggct gataacgtcg atttagataa
     4561 cgcggttgct aacgttgttc ccactcgtgg ggcgatcgtg cgagcagagt ttaaagcgcg
     4621 cgttgggata aaactgctca tgacgctgac ccacaataat aagccgctgc cgtttggggc
     4681 gatggtgaca tcagagagta gccagagtag cggcattgtt gcggataatg gtcaggttta
     4741 cctcagcgga atgcctttag cgggaaaagt tcaggtgaaa tggggagaag aggaaaatgc
     4801 tcactgtgtc gccaattatc aactgccacc agagagtcag cagcagttat taacccagct
     4861 atcagctgaa tgtcgttaag ggggcgtgat gagaaacaaa cctttttatc ttctgtgcgc
     4921 ttttttgtgg ctggcggtga gtcacgcttt ggctgcggat agcacgatta ctatccgcgg
     4981 ctatgtcagg gataacggct gtagtgtggc cgctgaatca accaatttta ctgttgatct
     5041 gatggaaaac gcggcgaagc aatttaacaa cattggcgcg acgactcctg ttgttccatt
     5101 tcgtattttg ctgtcaccct gtggtaatgc cgtttctgcc gtaaaggttg ggtttactgg
     5161 cgttgcagat agccacaatg ccaacctgct tgcacttgaa aatacggtgt cagcggcttc
     5221 gggactggga atacagcttc tgaatgagca gcaaaatcaa atacccctta atgctccatc
     5281 gtccgcgctt tcgtggacga ccctgacgcc gggtaaacca aatacgctga atttttacgc
     5341 ccggctaatg gcgacacagg tgcctgtcac tgcggggcat atcaatgcca cggctacctt
     5401 cactcttgaa tatcagtaac tggagatgct catgaaatgg tgcaaacgtg ggtatgtatt
     5461 ggcggcaata ttggcgctcg caagtgcgac gatacaggca gccgatgtca ccatcacggt
     5521 gaacggtaag gtcgtcgcca aaccgtgtac ggtttccacc accaatgcca cggttgatct
     5581 cggcgatctt tattctttca gtcttatgtc tgccggggcg gcatcggcct ggcatgatgt
     5641 tgcgcttgag ttgactaatt gtccggtggg aacgtcgagg gtcactgcca gcttcagcgg
     5701 ggcagccgac agtaccggat attataaaaa ccaggggacc gcgcaaaaca tccagttaga
     5761 gctacaggat gacagtggca acacattgaa tactggcgca accaaaacag ttcaggtgga
     5821 tgattcctca caatcagcgc acttcccgtt acaggtcaga gcattgacag taaatggcgg
     5881 agccactcag ggaaccattc aggcagtgat tagcatcacc tatacctaca gctgaacccg
     5941 aagagatgat tgtaatgaaa cgagttatta ccctgtttgc tgtactgctg atgggctggt
     6001 cggtaaatgc ctggtcattc gcctgtaaaa ccgccaatgg taccgctatc cctattggcg
     6061 gtggcagcgc caatgtttat gtaaaccttg cgcccgtcgt gaatgtgggg caaaacctgg
     6121 tcgtggatct ttcgacgcaa atcttttgcc ataacgatta tccggaaacc attacagact
     6181 atgtcacact gcaacgaggc tcggcttatg gcggcgtgtt atctaatttt tccgggaccg
     6241 taaaatatag tggcagtagc tatccatttc ctaccaccag cgaaacgccg cgcgttgttt
     6301 ataattcgag aacggataag ccgtggccgg tggcgcttta tttgacgcct gtgagcagtg
     6361 cgggcggggt ggcgattaaa gctggctcat taattgccgt gcttattttg cgacagacca
     6421 acaactataa cagcgatgat ttccagtttg tgtggaatat ttacgccaat aatgatgtgg
     6481 tggtgcctac tggcggctgc gatgtttctg ctcgtgatgt caccgttact ctgccggact
     6541 accctggttc agtgccaatt cctcttaccg tttattgtgc gaaaagccaa aacctggggt
     6601 attacctctc cggcacaacc gcagatgcgg gcaactcgat tttcaccaat accgcgtcgt
     6661 tttcacctgc acagggcgtc ggcgtacagt tgacgcgcaa cggtacgatt attccagcga
     6721 ataacacggt atcgttagga gcagtaggga cttcggcggt gagtctggga ttaacggcaa
     6781 attatgcacg taccggaggg caggtgactg cagggaatgt gcaatcgatt attggcgtga
     6841 cttttgttta tcaataaaga aatcacagga cattgctaat gctggtacgc aatattacct
     6901 gaagctaaaa acctgcacgt tagccctttg taggccagat aagacgcgtc agcgtcgcat
     6961 ctggcataaa caaagcgcac tttgctggtc tgttcccctc accctaaccc tctccccgga
     7021 ggggcgaggg gactgtccgg gcacattttt agactttgtc atcagtctga gcctgccatt
     7081 ggcaggctct ggtgtccttt tacgctacca tgctaataat cagcacaata atcagcccaa
     7141 ccacggagtt gaccagctcc agcagacccc aggttttcaa cgtgtctttt actgacaggt
     7201 caaagtaacc tttgaacaac cagaaagatg catcgttaat gtgggtgagg gtgttggaac
     7261 ccgcagccgt cgccagtacc agcagcgccg gattcacgcc aaccagctga ccagttgctg
     7321 gatctaggat tgcagcactg ataatcccgg cggcggtcat cgccgaaacg acaccctgac
     7381 ccgtcgccag acgaattagc acagtgatca gccatgccat gatgtagggc gagatattgc
     7441 cgtgggacat caacatgccg atggtgtcgc caatgccggt gtcgatgatg gtctgcttca
     7501 gcacgccacc cgcaccgatg atcagaatca ccattgcaat actcttcacc gcgctttcaa
     7561 aagcgttcat cacccactgc atgtcatgac cacgtgcggt gccaaagagt acgaatgcaa
     7621 ccaccatcgc aataaacatt gcaatcggcg aggaaccgat aaagttaacc acttcccagg
     7681 caggggtatc ttttaccagc cagatattgg cgatggtggt agagatcata atgatcgccg
     7741 ggatcagcgg caccagaatc gaaacgccga aagagggcag attattcata tctaccggtt
     7801 gatctgcttt caggaatgat ggcgttgggc gctcaagatt gccgaggaac ttcggcagga
     7861 tcagacctgc gcagattaca cttgggatcg tcaccagtac gccatagata taaaccatcc
     7921 ccatatccgc gccataagca ttcaccagcg ccaccggacc cggctgcggt gggaacagtg
     7981 aatgtgcggt agtggcagct gctactgccg ggatcgccag tttcaggaac ggaattttag
     8041 cttcggcggc aataacaata accagcggcg ctaacatgat aaaggccact tcatagaaca
     8101 tcgccagacc aaaaatcagg ccgatgataa tcaccgacag ctgtacatag cgcagaccga
     8161 gacgcgccag cagcgtatgc gctatctggt gagccgcgcc ggagtcgacc atcaatttac
     8221 cgatgaccgc accgaacacc acgatgatag ccagttcccc cagcgtgttg ccgaagcccg
     8281 ctttcatagt gtgcagcagc gacatcaaat ccatgcccgc cagcatcccg acggacagcg
     8341 ccgccaccaa caaagccacc attgaattga ttttgaactt caaattcagt accagcatca
     8401 gaccaatgcc gaataccacc cagagaatgt taagcacatg cataacgttt taccttacct
     8461 ggttgaaccg ttgttatttt gggcgatatg ttatgtaaat tggtcaacca ttgttgcgat
     8521 gaatgtcaca tcctctgatc aataaccatc gattaccctt tgctgcaatt tgcagcaaca
     8581 accatgagag tgaaattctt gtgatgtggt taaccaattt cagaattcgg gttgacatgt
     8641 cttaccaaaa ggtagaactt atacgccatc tcatccgatg caacgccacg gctgcggtct
     8701 ggttgttcat ccggatacct aaacaactcc agggttccgc gtctctttgc tgtggaaccc
     8761 actatgtgaa agaggaaaaa tcatggaaca gacctggcgc tggtacggcc caaacgatcc
     8821 ggtttcttta gctgatgtcc gtcaggcggg cgcaactggc gtggttaccg cgctgcacca
     8881 tatcccgaac ggcgaagtat ggtccgttga agagatcctc aaacgcaagg cgatcattga
     8941 agacgcaggc ctggtgtggt ctgtcgtaga aagcgtgcca attcacgaag atatcaaaac
     9001 ccacactggc aactatgagc agtggatcgc taactatcag cagactctgc gcaacctggc
     9061 gcagtgtggc attcgcaccg tgtgctacaa cttcatgccg gtgctcgact ggacccgtac
     9121 tgacctcgaa tacgtgctgc cagacggctc caaagctctg cgcttcgacc agatcgaatt
     9181 cgctgcattc gaaatgcata tcctgaaacg cccaggcgcg gaagcggatt acaccgaaga
     9241 agaaattgct caggcagctg aacgcttcgc cactatgagc gatgaagaca aagcgcgtct
     9301 gacccgtaac atcattgctg gtcttccggg cgcggaagaa ggctacaccc tcgaccagtt
     9361 ccgtaaacac ctggagctgt acaaagatat cgacaaagcg aagctgcgcg aaaactttgc
     9421 cgtcttcctg aaagcgatta ttccagttgc tgaagaagtc ggcgtgcgta tggctgttca
     9481 cccggacgat ccgccgcgcc cgatcctcgg cctgccgcgc attgtttcca ccattgaaga
     9541 tatgcagtgg atggttgata ccgtaaacag catggcaaac ggttttacca tgtgcaccgg
     9601 ttcctacggc gtgcgtgctg acaacgatct ggttgatatg atcaagcagt tcggtccgcg
     9661 tatttacttc acccatctgc gctccaccat gcgtgaagat aacccgaaaa ccttccacga
     9721 agcggcgcac ctgaacggtg acgttgatat gtacgaagtg gtgaaagcga ttgttgaaga
     9781 agaacaccgt cgtaaagcgg aaggcaaaga agacctgatc ccgatgcgtc cggaccacgg
     9841 tcatcagatg ctggacgacc tgaagaagaa aaccaaccca ggttactccg caattggtcg
     9901 tctgaaaggc ctggccgaag ttcgcggtgt cgaactggcg atccagcgcg ctttctttag
     9961 ccgttaatat ccaccggcat ggctgcgcgc cgtgccggtt ccttcttcct tgccgtcact
    10021 ctttgaagac ggattctgga gtttacgatg actactattg ttgacagcaa tctgccggtt
    10081 gcccgcccgt catgggatca ttctcgtctg gaatcacgca ttgtgcatct cggttgcggg
    10141 gcgtttcacc gcgcgcacca ggcgctgtat acccatcatc tgctggaaag caccgacagc
    10201 gactggggca tctgcgaagt taacctgatg ccaggcaacg accgcgtgct gatcgaaaac
    10261 ctgaaaaaac agcaactgct gtacaccgta gcggaaaaag gcgcagagag caccgagctg
    10321 aaaattatcg gttcgatgaa agaagcgctg catccggaaa tcgatggctg cgaaggtatt
    10381 ctcaacgcga tggcgcgtcc gcaaacggcg attgtctctc taacggtcac ggaaaaaggc
    10441 tactgcgctg atgcggcaag cggtcaactg gatctcaata acccgctgat caagcacgat
    10501 ctggaaaacc cgactgcgcc gaagtccgcg attggttaca tcgtcgaagc gttgcgtctg
    10561 cgtcgtgaaa aagggttgaa agcgtttacg gtgatgtcct gcgataacgt gcgtgaaaac
    10621 ggtcatgtgg cgaaggtcgc ggtactgggg ctggctcagg cgcgtgaccc gcagctggcg
    10681 gcatggattg aagaaaatgt caccttcccg tgcaccatgg ttgaccgcat cgttccggcg
    10741 gcgacgccag aaaccataca ggaaattgct gaccagctgg gtgtttacga cccgtgcgcc
    10801 attgcctgcg aaccgttccg tcagtgggtg attgaagata acttcgttaa tggtcgcccg
    10861 gattgggata aagtgggcgc acagttcgtt gcagacgttg tgccgttcga aatgatgaag
    10921 ctgcgtatgc tgaacggcag ccactctttt ctggcgtacc tcggttacct cggcggctat
    10981 gaaaccattg ccgacaccgt gactaacccg gcttatcgca aagcggcctt tgccctgatg
    11041 atgcaggaac aagcgccaac gctgtcgatg ccggaaggta cagacctgaa cgcctatgcg
    11101 acgctgctga tcgagcgttt cagcaacccg tctctgcgtc accgtacctg gcagattgcg
    11161 atggacggca gccagaagtt accgcagcgt ctgctggacc cggtgcgtct gcacctgcaa
    11221 aacggcggca gctggcgtca cctggcgctg ggcgtggctg gctggatgcg ttacacccag
    11281 ggcgtggatg agcagggtaa tgccattgac gtggtcgacc cgatgctggc ggagttccag
    11341 aagatcaacg cgcagtatca gggcgcagac cgcgtgaaag cgctgctggg cctgagcggt
    11401 atttttgccg atgatctgcc gcagaatgcc gactttgttg gcgcagtgac ggcggcatat
    11461 cagcagctgt gcgaacgcgg tgcgcgcgag tgtgtggctg cgctgtaact aactgattac
    11521 cctacagact tactggtcaa tcaaactgat atttggttga ccagttttcg tttttttgcc
    11581 cacctgtacg tgccaacttc cagtgctaat ggtatagttt gatattaacg ggggccgtaa
    11641 aattgcccgt tgtaggccgg ataaggcgtt cacgccgcat ccggcaaaaa tttgattaac
    11701 cgcacctaac ggacacaaca ccatgaaatc tgccacctct gcgcaaagac cttaccagga
    11761 agtcggggcg atgatccgcg atctgatcat aaagacgccg tacaatcctg gcgaacggct
    11821 gccgccggag cgtgaaattg cagaaatgct tgatgtcacg cggacggtgg tacgtgaagc
    11881 gctgatcatg ctggagatca aagggctggt ggaagtacgc cggggtgccg gtatctatgt
    11941 tcttgataac tcaggcagcc agaacacaga cagtccggat gccaacgtct gcaacgatgc
    12001 cggtcctttt gagctgttac aggcgcggca gttattggag agcaacatcg ccgagtttgc
    12061 cgctttgcag gctacccgcg aagatatcgt caaaatgcgt caggcattgc aactggaaga
    12121 gcgtgaactg gcttccagtg cgccgggcag cagcgaaagc ggtgacatgc agttccatct
    12181 cgctattgcc gaagcaacgc ataacagcat gctggtggag ctgttccgtc agtcctggca
    12241 gtggcgggaa aacaatccaa tgtggatcca gttgcacagc catctggatg acagcctgta
    12301 tcgcaaagag tggttgggcg atcacaaaca gatcctcgcc gcgttaatca aaaaagatgc
    12361 ccgagcggcg aagctggcaa tgtggcagca tctggaaaac gttaagcaac gtctgctgga
    12421 attctcgaac gttgacgata tttattttga tggctatctg tttgattcat ggccgctgga
    12481 taaagtcgac gcctgactta ttataataag cgcaagggta aacgttcctt gcgctttctt
    12541 aaattaagaa gtcgcaatga gtattacttt gtaaattgca gggtattgtt tagctatctg
    12601 tataacctga atgttagtac tcattcttcc tggtagttat ttaccaatat aattccattc
    12661 accatttttt aattcaaaca gttctggaag agatgacggt tgcagagtca tacgtttgag
    12721 tggtgcattt tcatcacccg ggataacttt atatcccatt tttgcatgga tctccgcggt
    12781 accgggatag gcttctatac caattattgg gtcattaccc aactcaatat tagcattaag
    12841 taatgcggca agggcgaatc cggaagaatt tgctacccct gttaccgtca ttatctgatc
    12901 aatatagata tcaatccttg gctgctcatt ggtccatctt tttgcctgag aagctggaag
    12961 ggttgaaata tatcctatcg tggttccggc ataaccagga acattgcaac cagggcctaa
    13021 tgttatttca tgaactgtat agtcctcaac aggatcgaaa gggctcttca taaataaatt
    13081 tagccgtaaa ccttctgcta actctgtggt agcatgcgcc tttttacata tgccaccata
    13141 tatttcgtct aaagctgagg tgattttcaa gttccgatag agcatacgtc ctgttgttgt
    13201 aataccatcg gattctctgc atgctcctgt cacataatta aatggccaag gctctttact
    13261 aatatcaagt tttctaattt cgacaacgct ttttccatcg taattatggt tattgccacg
    13321 agtaagaggc ggaagataga tgttttccga atccatgcgt gcatatttta atttacgtgt
    13381 aaactcactg tcgactattg ggtgatgaag gaagtgagca tctgtaataa tttgactctg
    13441 aagggcattg cttaatggca tagacataat agttaccttt tatgagtatt tcactgatgt
    13501 ttagaaaaat agataaattt ttctgtagta aaaagagaag taaacaaatg acatgcatgt
    13561 ttctgttgta gtgatatcaa ctctacacgg tgatattaaa gggtaggaaa cactctaaag
    13621 tatcaaaaaa acgctcattt aaaatt
//
LOCUS       DAZFRE010000114        13643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFRE010000114 DAZFRE010000000
VERSION     DAZFRE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.114"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..92
                     /locus_tag="ACHKUI_004168"
     CDS             <1..92
                     /locus_tag="ACHKUI_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600251.1"
                     /translation="AFAIKWVAVGVAVSPMLYGLAKLVIALKS"
     gene            107..238
                     /locus_tag="ACHKUI_004169"
     CDS             107..238
                     /locus_tag="ACHKUI_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600252.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFFSTAWRVLTLSGLVG"
     gene            246..713
                     /locus_tag="ACHKUI_004170"
     CDS             246..713
                     /locus_tag="ACHKUI_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6600253.1"
                     /translation="MSRVLCVVIIVLAGGCGALWLATDHYRDNAITYKVQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGMDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCLN"
     gene            890..1441
                     /locus_tag="ACHKUI_004171"
     CDS             890..1441
                     /locus_tag="ACHKUI_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6600254.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            1741..2547
                     /locus_tag="ACHKUI_004172"
     CDS             1741..2547
                     /locus_tag="ACHKUI_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6600255.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLEWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDAGSDDEKCAKNSEKNCANSAETKRNSRSGNP
                     HPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTGTLKKM
                     FADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2528..4234
                     /locus_tag="ACHKUI_004173"
     CDS             2528..4234
                     /locus_tag="ACHKUI_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6600256.1"
                     /translation="MTFRKNKPRCDESAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLALTPQDYKFHFYAWWQDLKYSARVPESGLKLSQEKTAYFSAVEKAMN
                     ITLTDEQKHWYICKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGTKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGLSGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4234..6378
                     /locus_tag="ACHKUI_004174"
     CDS             4234..6378
                     /locus_tag="ACHKUI_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6600257.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLLVISDDPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMHAVAVASGRVQVRVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSNASSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEAAAQQQALEQ
                     QQAELQMREMTGRVAKLEAEAARARAAAQRDNAGAQRDVAVAQGQRYVDALNQAHTAE
                     IITGIQNMEQEQEVLQQQMLHTLQQRMHEMPL"
     gene            6536..7543
                     /locus_tag="ACHKUI_004175"
     CDS             6536..7543
                     /locus_tag="ACHKUI_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600258.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDGHEQDGVTEAATENHAD
                     EAAQTQTGDNTAAPTPDASAEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRLVELLTSQIHQAGMKPAPLPENEKISDEKIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGHGGEDLSPVLDAMNAVPVLKTWQNSDPDRFSVA
                     VSIDGKLQNDPAWKDKPLTERFAEVARRTQLAFGEASEPPSDDKADNTDIRKTAEEKV
                     KAAEREMAVPASPSELGNTASVGTGDNFERLLGASHTEIEAAMRGMTDAEIDAILEKL
                     G"
     gene            7566..8780
                     /locus_tag="ACHKUI_004176"
     CDS             7566..8780
                     /locus_tag="ACHKUI_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO6600259.1"
                     /translation="MTTVTTAQANKLYQVALFTAANRNRSMVNILTEQQDAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTADHPEFKKIMINDVLPPTHDRHFFGGDATSFEGIDQSDLFTLALVDNLSL
                     FIDEMAHPLQPVRLSGDELYGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMTRAVNRS
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSKNDLTATTEEKQAATNIDR
                     AMLLGAQALANAYGQKGGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGRMQD
                     HGVIAVDTAVKL"
     gene            8835..9227
                     /locus_tag="ACHKUI_004177"
     CDS             8835..9227
                     /locus_tag="ACHKUI_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600260.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLFS
                     LPIGVRINALQLVSTKGLGGTATVSVKSGEHELIGDSGAVTASFAKYVPVEPYTTQRD
                     GELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            9278..9739
                     /locus_tag="ACHKUI_004178"
     CDS             9278..9739
                     /locus_tag="ACHKUI_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600261.1"
                     /translation="MSEKIAVVYIGPKPVKKDTLTGSRTLFPRLEPVHVDSALAWQLL
                     AFPDVWVRHEELDGVLKKQQQDEQLRQAQQAQEREQVALTEAENSFVVSVGGQDVDLS
                     KLTSARLATLCEAEELNIHKDPKETADAFRVRVREAFRRRVAETEQHGGTD"
     gene            9723..10286
                     /locus_tag="ACHKUI_004179"
     CDS             9723..10286
                     /locus_tag="ACHKUI_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600262.1"
                     /translation="MAELTDFLPYVRRHISGPLSIMMTDALSMSAVAFCRQSLLCRRE
                     ATLSPSAGEDCVLPYDAENEECVHIIRILADGRELFAGHDVEIRPGRVMHFACSPGEV
                     SVLYAVAPKAGSRQVQDELRAWSEEVAAGALERLFMQTGVSWSDPSRAQYFSVLFSEG
                     IRRAYRDTLATSPYSSYRNPVRRQRFY"
     gene            10286..10936
                     /locus_tag="ACHKUI_004180"
     CDS             10286..10936
                     /locus_tag="ACHKUI_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6600263.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLNCVPGARQTLPDGAIQLLDVICLSDGSAIKPQSREVLDAQYPDWHMLRG
                     KPECFISSDLAPRVFWLFPAPEEAVSVDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGM"
     gene            10933..12867
                     /locus_tag="ACHKUI_004181"
     CDS             10933..12867
                     /locus_tag="ACHKUI_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6600264.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     DGGYYGMTLEPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTAVKNNQASQSKESAAASAKTASESAKAATSKAT
                     ESAQKASEAAEAATRAGESEKAAAADAKEARRHAETARVAQEAAGDVLKRAEAATVSA
                     EEARRMAENARGPKGDTGPKGDAGPRGETGPAGPRGEKGEQGERGPQGIPGQKGDTGE
                     RGPQGVQGPAGATGPSGPRGATGPQGAMGPRGETGARGEKGDPGGPQGPKGDAGPRGE
                     PGPKGDTGPRGEAGPPGPQGPAGQTGPKGDKGEPGATGPAGPAGPRGETGPAGPAGPA
                     GSVASVPDASTSQKGVVQLSSDTNSDDETKAATPKAVKAVMAEVQAAKTKAEEAATRA
                     AVPGPKGDRGEPGAPGAVGPAGPRGPAGAAGPKGDAGPAGPRGERGPAGAQGVPGPVG
                     PAGPAGKTGPRGLQGETGRQGPTGPQGPTGEPGPQGPQGPTGRLVPGEIYSIGTYIIA
                     ALAPYITDIGRTYQPGETVSGSRLKRCALIKDENGNYMKANTDGIDGNLSSVPGSWMV
                     CNEITSTNDNESIGLFQRAY"
     gene            12884..13465
                     /locus_tag="ACHKUI_004182"
     CDS             12884..13465
                     /locus_tag="ACHKUI_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6600265.1"
                     /translation="MNVEKISNPQWADKEHTAVNCMVKFEHIEQAVPFTATASDTEAY
                     GRDIYAACLRGEAGEIAEYVQPSISPEKARELKTAGINAWRNAMEAANYTFEHNGRKW
                     DYGKSTQTRLEPSVAAAKAGKLPEAFFWTDADNNDVPMDAETLIALSAAAEQAMFTKG
                     MEIHVRQRTMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT         3491 a         3227 c         4021 g         2904 t
ORIGIN      
        1 tggcttttgc cattaagtgg gtggcggtcg gtgttgctgt gtccccgatg ctgtatgggc
       61 tggcaaaact ggtcattgcg ttgaaatcgt gaagggagga ttaagcatgt cagacaaact
      121 cataacgctg gcgaagatcc tctgtgtaat tgtcggcatt tcattttcac taatgctggt
      181 tgccattttc ttttccaccg cctggcgagt cctgacgtta tcgggactgg tggggtgaaa
      241 gagagatgag ccgtgttctg tgtgtggtga ttattgtcct ggcgggtggc tgtggtgcgc
      301 tgtggctggc aacagaccat taccgcgata acgccatcac ctacaaagtg cagcgcgata
      361 aaaaagccag agagctggag ctggcaaacg ccaccattac tgatatgcag gtgcgccagc
      421 gcgatgttgc tgcgctcgat gcaaaatact cgagggaatt agccgatgcg agagctgaaa
      481 atgaaactct gcgtgctgat gttgctgctg gtcgtaagcg cctgcggatc aacgccacct
      541 gctccggtac cgtgcgtgaa gccaccggca cctccggcat ggataatgca accggccccc
      601 gactggcaga caccgctgaa cgggattatt tcatcctcag agaacggttg atgacaatgc
      661 agaagcagct ggaaggggca caggaatata tccgcactca gtgcctgaat taacagagcc
      721 agcttaatcg ctggcttttt catatctgaa tttcatcgcg catctcacgc gcatatcaca
      781 tccccgagcc tttcagaaag ttgagcctga gaactgccgt atatggtggc gaccatctcg
      841 gggcggcttt tctgtgagac aggctcaatt ttctgaaagg taagacgcta tgaactatcc
      901 gacgattgtt aacggtattg atttccgcga tcttgttttt gtgacaggca acgagccggt
      961 gacggatacc cgaaaggttg ccgtagcatt tggcaaagaa cacaaagatg tattgcggaa
     1021 aacaagagcc gttgttcaac aatgctcaaa agaatttgca gagcgcaatt ttgcgctttg
     1081 ctatgaaaac aatgagttac agaacggtaa accccagccc atatatcgaa tgacaaaaga
     1141 cggttggacg atgttggtat ttggttttac ggggaaagct gctatgacct ttaaggaagc
     1201 gtacattcag gctttcaact ggatggctga gcttattcag caaggtctgg cgaatctgga
     1261 ggcggagcgc aacgctgtaa tgctggagta catgaaagag aaagatgtcg ccagtatgtc
     1321 aggtcgtttg ctcaatcgct ggggcagggt gaagaagcct cagttgctgg caaggctgga
     1381 caggctggag cagcaggggc agattgcgtt acccggattt gataaaggca tttcagcctg
     1441 acatagccat gcgccgtatc gtcgcagtat tcccgcatta acagagacct cagccagaca
     1501 ctggacctcc tgcgcgagtg tgtggggata atcaaaaacg atgcacaccg ggctttccac
     1561 gttttcaggt aacgtgagcg ttttcccgca tggccgcctg tccggtgcgg tggtggaaga
     1621 aaccggataa aacaaccata ttgtgtaaat atcaatcaaa caagacgctg ctgtgtgaat
     1681 tctgaaaaat cacagcggtc attatgcgtc agtttttaac agaggacgtc agaaagtgac
     1741 atggcaaagc tggactggaa aaaactggag caggcattcc gacgcgaaca tgccgaaacg
     1801 ggaataacat tactggagtg gtgccgtaag aaaaagatta attacaacac cgccagaacc
     1861 cgtataaaaa tgggcaaaat cgatcatgaa atcgatcata aaaccgatca tgaaatcgat
     1921 catgacatct cagatgaaga acccagcaat gacgcgggtt ccgacgatga aaaatgcgca
     1981 aaaaattctg aaaaaaactg cgcaaattcg gcagaaacga aacgcaacag cagatcgggc
     2041 aatcctcacc ccgttgcccg gtttagcgac cgtaacaccc acgccgtgag acaccgtgga
     2101 tatgcgaagt atcttgaggc agataacctc atggatgatg cgtccgatat ggtgctgttc
     2161 gatgaactgg tgttcacccg tgcccgcgca ctttcagtaa caggaacact gaaaaaaatg
     2221 ttcgccgacc tgaaagaggc ggctgacgtg gaaacccgtg ttgccctgta cgacaaaata
     2281 cttaaagccg aacaggctct tgaccggaat attgcccgta tcgagtcaat tgaacgctca
     2341 ttgctgacgc tggacgttct ggcggaaaca gcaccaaaac ttcgtgctga ccgggaaaga
     2401 atcaacgctg ccagagataa actcagagcg gaaaccgata ttctgaccag ccagcgtcgg
     2461 ggcgttgtta cgcctgtcag tgacatcgtg tcatcgctgc atgaaatgag taattcgggg
     2521 agactggatg acattccgga agaataaacc gcgatgtgat gaatctgcag aaatgactga
     2581 gaccgaacaa cgtctgttca ttatgacaaa actgagcaat ccctggtggc gcctcaatca
     2641 tctctacaag atacagaacg aaaaaggtga gctggtcacc ttcagaatgc gaccggcgca
     2701 gcgccagttg ttccggagca tgcacaataa aaatattatc ctgaaagcac gccagctggg
     2761 gttttccacg gccattgata tttatcttct cgaccaggca ttattcatac cgcatctcaa
     2821 atgcgggatt gtcgctcagg ataaacaggc tgccagtgaa attttccgca ccaaaattgc
     2881 tgtaccgttt gatcatcttc ctgactggct gagagcctca ttcaccatcg ttgaacgtcg
     2941 tagcggagcc agcggtggct atatcctgtt tggtcacggc tcgagtatcc aggtggcaac
     3001 ctcattccgt tcaggtacgg tgcagcgcct gcatatctca gagcacggca aaatttgcgc
     3061 aaaatatccg gctaaggcga aagaactgcg aaccggtaca cttaatgccg tctctgatga
     3121 gtgcattatt tttgatgaat ccactgctga aggtgtgggt ggcgattttt acgagatgag
     3181 taaccgggca caggaaatca ctgcatcagg gctggcgctg acgccacagg attataaatt
     3241 ccatttttac gcctggtggc aggatctcaa atacagcgcc agagtgccgg aaagcggact
     3301 taagctgtca caggaaaaaa cagcgtattt ttctgcggtt gaaaaagcaa tgaacatcac
     3361 gcttaccgat gagcagaaac actggtacat ctgcaaggaa actgaacagc gtgaggaaat
     3421 gaagcaggaa tttccctcaa cgccacagga ggcgtttctg acgtcaggac gacgggtgtt
     3481 cagtgcggaa agcacgctgc aggcagagtc attctgttcg ccaccgctga ttgtttatga
     3541 cattgaaccg gttacaggaa cgaagaccaa agcgcagtct ctgcgtgacg ggaataaagc
     3601 cgaacagcac cggacgctga tgaattatct gctggtctgg gagctgccgg atccggatga
     3661 agagtatgta tgcggagcgg ataccgctga ggggctggag cacggagacc gctcatcgct
     3721 ggatatcatc agatgcagta atggtgagca ggtggctcac tggtttggtc atctcgatgc
     3781 ggagcttttc gctcatctca ttgcgcaggt ctgccgtatg tacaacaatg cgtttgtggg
     3841 gccggagcgt aacaatcacg gacatgccgt tatcctgaaa ctcagggaac tctatccgac
     3901 gcgttatatc tacaacgaac agcaccttga ccaggcatat gacgacgata cgccccgtct
     3961 tggctggctg acaacccgcc agagtaaacc ggtcctgaca gagggaatga aaacgctgct
     4021 gaataacggc ctgtcgggga tccgctggtc gggcacatta tcagaaatga acacctacgt
     4081 ttatgacgcg aaaggctcca tgaatgcaca ggaaggctgt tttgatgatc agcttatgag
     4141 ctacatgatt gcccaggaga tgcgcgccag aatgccggtg agggtaaaac agaaaacgga
     4201 taaacgcaga accacacact ggatggctca ctgatgaaaa atgaaactaa taccatggcg
     4261 acgaaaaacg acaacggagc cacgccgcgt ttttctcagc gccagttaca ggcgctttgt
     4321 tctgatattg acagtcagcc taaatggcgc gatgccgcaa acaaggcctg tgcgtattac
     4381 gatggtgacc agttgccacc ggaagttctc caggtgttga aagatcgcgg tcagccgatg
     4441 accatccata acctcatcgc gcctaccgtt gatggtgttc tgggtatgga ggccaaaacg
     4501 cggactgatc tgttggtgat atcagatgat cctgatgatg aaacagaaaa actggctgaa
     4561 gctattaatg ctgaatttgc cgatgcttgc cgccttggaa atatgaataa agcccgttct
     4621 gatgcctatg cggaacaaat caaagcgggg ctcagctggg tggaggtcag acggaacagc
     4681 gatccgttcg ggcctgagtt taaggtgtct actgtcagcc ggaatgaagt gttctgggac
     4741 tggctcagcc gggaggctga tttaagtgac tgccgctggc tgatgcgccg ccgctggatg
     4801 gataccgatg aggcaaaagc cacatttccg ggaatggctc aggttatcga ttatgccatt
     4861 gatgactggc gtggttttgt cgataccacg gttactgaag gccagcccag tccgttgatg
     4921 agtgcatggg aagagtatca gtcatgggat cgacagcaga acgaatggct tcagcgtgaa
     4981 cgccgtcgtg tgctgcttca ggtggtttat taccgtacat tcgagcgtct tccggtgatt
     5041 gaactcagta atggacgggt ggtggccttt gataaaaata atctgatgca tgcggtggct
     5101 gtggcatccg ggcgggtgca ggtgagagtc gggcgggtaa gtcgtattcg tgaagcctgg
     5161 tttgtgggac cgcactttat tgtggatcgc ccctgtagtg cgccgcaggg gatgttcccg
     5221 ctggttccct tctggggata ccggaaggat aaaaccgggg agccttacgg gctgatttcc
     5281 cgggccattc cggcacagga tgaggtgaat tttcgtcgta taaaactgac gtggttgctt
     5341 caggccaaac gcgtgattat ggacgaggat gccacccagt tgtcagacaa cgatctgatg
     5401 gagcagatcg agcgtccgga tggcattatt aaactgaatc cggcccgaaa aaatcagaaa
     5461 agcgttgctg atgttttccg ggttgaacag gattttcagg ttgccagcca gcagtttcag
     5521 gtcatgcagg agtcggaaaa acttatccag gacacgatgg gggtttattc cgcatttctc
     5581 gggcaggatt caaatgcatc gtccggtgtg gcgatcagca acctggtgga gcagggagcc
     5641 acaacccttg ccgaaatcaa cgataactac cagtttgcct gccagcaggt gggaaggctg
     5701 ttgctcgctt atcttcttga tgacctgaaa aagcggcgta atcatgcggt ggtgattaat
     5761 cgcgatgatc gtcagcgtcg ccagaccatt gtcctcaatg ctgaaggtga taatggtgaa
     5821 ctgaccaatg atatttcaag gttaaataca catattgcgc tggctcctgt tcagcagaca
     5881 ccggcgttta aggcacagct tgcacagaga atgtcagagg ttattcaggg gctgccgcct
     5941 caggtgcagg ctgttgtgct cgatctgtgg gttaatcttc tggatgtgcc gcagaaacag
     6001 gaatttgtcg aacgtattcg tgcggcgctg gggacgccaa aatcaccgga tgagatgact
     6061 ccggaagagc aggaagcggc agcacaacag caggcgctgg agcaacagca ggcagaactt
     6121 cagatgcgcg aaatgacagg ccgggtggct aaactggaag cagaagctgc cagggcacgc
     6181 gcggctgcgc aacgggataa tgctggcgca caacgggatg tcgccgtggc acagggacag
     6241 cgttatgtgg atgcgcttaa ccaggcacat acggcagaaa tcattaccgg catacagaat
     6301 atggaacagg agcaggaagt tcttcagcaa cagatgctgc atacactaca acagcggatg
     6361 catgaaatgc cgctctgaaa atcctgaact taacggaacc ccgtcatcgt acggggtttt
     6421 ttgtttccgg agataagcgt tccgggagcg gtgcgcttat tcgcgggggc agcgataagc
     6481 cttatttact caaccattcg gatctgtccg ataaacagac catgcggagt tatttatgga
     6541 ttttgaattt acgggtgaag aaaccccgga acaactggaa aaaatgctgg aagggcttgg
     6601 ggatgtggat attgacggtc acgaacagga cggcgtgaca gaggccgcca cagaaaatca
     6661 tgcggatgag gcagcacaga cacagacggg cgataacact gcggcaccga cgccggatgc
     6721 cagtgctgag cagacgcagg acgtgaagga gccggaggcg aagggtgtgc tcacccgcga
     6781 tggtaaacac gtcattccct atgaagtcct tgaggctgaa cgttccggta agcagcgggc
     6841 cgaacaggaa gccgcacttc ttcggggaca gatagccgaa gaaaaacgcc tggttgaact
     6901 gctgacgtct cagatccacc aggccggtat gaaacccgca ccgttaccgg aaaacgaaaa
     6961 aatctctgat gagaagattg cccgtatcag ggagatgtat ccggaaattg gtgacgcggt
     7021 ggcttcgctc atccgtaaaa ataactatct ccagtcccgt gttcagcaat cagcacagca
     7081 ggcagaaggt catggtggtg aggatttatc accggttctt gatgcgatga atgccgtgcc
     7141 ggtgctgaaa acgtggcaga actccgatcc ggatcgcttc tcggttgccg tatccatcga
     7201 cgggaagctc cagaatgacc ccgcatggaa agacaaaccg ctcactgaac gtttcgccga
     7261 agtggcccgc cgtacgcagc tcgcttttgg tgaagccagt gagccgcctt ctgatgacaa
     7321 ggcagacaac acggatatcc ggaaaacggc ggaagaaaaa gtgaaagccg ctgaacggga
     7381 gatggcagtg cctgcttccc cgtcagagct tggcaacacg gcttccgtcg gaaccggtga
     7441 taattttgaa cggttacttg gtgcttccca taccgaaatt gaagccgcca tgcgaggcat
     7501 gacggacgct gaaattgacg cgattctgga aaaactcggg taaccactga aggagtactg
     7561 acgtaatgac cacagtaaca acagcccagg cgaataagct gtatcaggtg gcgcttttca
     7621 cggctgccaa ccgcaaccgc tcaatggtga atatactcac tgaacagcag gatgcaccaa
     7681 aagcagtttc gcctgacaag aaaagcacga agcagaccag tgccggtgca ccggttgtgc
     7741 gaatcacgga cctcaacaaa caggccggtg atgaagtgac attcagcatc atgcacaaac
     7801 tctctaaacg tccgacgatg ggggatgagc gcgttgaagg ccgtggtgaa gacctcagcc
     7861 atgctgattt ttccctgaaa attaatcagg gccgtcacct ggtggatgcg ggcggacgta
     7921 tgagccagca gcgtacgaag ttcaacctgg catcctctgc cagaacgctg ctgggtacgt
     7981 actttaacga cctgcaggac cagtgtgcga tagtgcatct tgccggtgcc cgcggtgatt
     8041 ttgttgctga tgacaccatt ctgccaacgg cagatcaccc tgaattcaaa aaaatcatga
     8101 tcaacgacgt actgccgccg acgcatgatc gtcatttttt cggtggggat gcgacgagtt
     8161 ttgaaggtat tgatcagtct gacctgttca ctcttgcgct ggtcgataat ctctcactgt
     8221 ttattgacga gatggctcat ccgttacaac cggtacgcct gtccggtgat gagctgtatg
     8281 gcgaagatcc gtattacgtc ctctacgtca cgccgcgtca gtggaatgac tggtacacct
     8341 ccacgtccgg caaggactgg aaccagatga tgacgcgtgc cgtaaaccgc tcaaaagggt
     8401 ttaaccatcc gctgttcaaa ggcgaatgtg cgatgtggcg caatatcctg gtccgcaagt
     8461 atgcggggat gccgatccgt ttctatcagg ggtcaaaggt tctggtatcg aaaaacgacc
     8521 tgacggcaac cacagaagag aagcaggccg caaccaacat tgaccgcgcc atgctgctgg
     8581 gtgctcaggc actggcgaat gcgtacggtc agaagggcgg cggtcacttc aacatggttg
     8641 agaagaaaac ggatatggat aaccgtactg agatagcaat cagctggatc aacggtctga
     8701 aaaaaatccg tttcccggag aagagcggca ggatgcagga tcacggtgtt attgccgttg
     8761 ataccgcagt gaagctctga tttttccttt ccccatgccg ggttatcgcc cggctttttc
     8821 aggagtcatt aattatggca aagaccatcc ttgccccgtc actgagtgaa cgggtctata
     8881 cgggtacgca cggtaatgag tcggtggcag aaggtgtgtt tactgtgaat gctgcggaag
     8941 cggacagtgt tattcatctt ttttcgttgc cgatcggtgt tcgtattaat gcgttacagc
     9001 tggtttctac caagggactt ggcggcacag caactgtcag cgttaaatct ggcgaacatg
     9061 aactcattgg tgacagtggt gcagtaactg ccagctttgc taaatatgtg ccagtggagc
     9121 cgtacaccac acagcgtgac ggagagctgg ttacggtcac cattaagaca gctgctgcga
     9181 ccggtaccct gaatgttctg ctgcgttata ccgtggtggg atactgatat aaaccttccg
     9241 gcccgcgtca tgcgggcttt tttatccggg gaattatatg agcgagaaaa ttgccgttgt
     9301 ctatatcggc cccaaacccg tgaaaaagga tacccttacc gggagtcgta cactgttccc
     9361 gcgtcttgag ccggtgcatg ttgacagcgc gcttgcctgg cagttgctgg cctttccgga
     9421 tgtctgggtt cgtcatgaag agcttgatgg tgtgctcaaa aagcaacaac aggatgagca
     9481 gttgcggcag gcacagcagg cgcaggaaag agaacaggtt gctcttacag aagcggagaa
     9541 cagttttgtg gttagcgttg gtggtcagga tgtggatttg agcaaactca cgtccgcacg
     9601 gctggcaacg ttgtgtgagg cagaagagct gaatattcac aaagacccga aagaaacggc
     9661 tgatgcattt cgtgtccgtg tgcgtgaggc atttcgccgt cgtgttgcgg agactgaaca
     9721 gcatggcgga actgactgat tttttaccgt atgtccgtcg ccatatcagc ggtccgctga
     9781 gcattatgat gacggatgca ttatccatgt cggcagtggc attctgccgt cagtccctgt
     9841 tgtgccgccg tgaagccacg ctgtcaccgt cagccggaga ggactgtgtg ctgccatatg
     9901 acgcggagaa tgaagagtgc gtacatatta ttcgtatcct cgctgacggg cgtgagcttt
     9961 ttgccggtca tgatgtggaa atccgcccgg ggcgggtaat gcattttgcc tgttcgcccg
    10021 gagaggtgag tgtgctttat gccgttgctc cgaaagcagg gagccgtcag gtgcaggatg
    10081 aactgcgggc atggtctgaa gaagtggctg caggggcgct tgaacggttg ttcatgcaga
    10141 ccggggtttc atggtcagac ccgtcgcgcg cacagtattt ctctgtgctg ttttctgagg
    10201 gtatccgtcg ggcatatcgc gacacactgg cgacaagtcc gtactcctca taccgcaatc
    10261 ctgtccgcag acaaaggttt tactgatgac gacgattact gaaatcatcg gacgagtgaa
    10321 cacgcaactg gttgacccga tgatggtgcg ctggcctctg gctgagctgt gtgattatta
    10381 caatgatgct gtgcgggcgg tgattctggc aaggccggat gcaggcgcaa gtctggagac
    10441 gctgaattgt gttcctggtg cacgtcagac tttgcctgat ggcgcaatac agcttcttga
    10501 cgtgatatgc ctcagcgatg gcagtgctat aaaaccgcaa tcccgtgagg tgcttgatgc
    10561 acagtatccc gactggcaca tgctgagggg gaaaccggaa tgctttatca gcagtgacct
    10621 tgccccgcgc gtgttctggc tgtttccggc accggaggag gctgtgagtg ttgatgctgt
    10681 ggtcagccgc attccggagg cggtgtatgt tctgacgcag gatgatgaca cgcctgtacc
    10741 gctggaagag gcttatgtta acccgctggt tgactggatg ttgtttcgtg cattcagcaa
    10801 ggatgcagcc ggtggtgcag agtcaggact ggcagcacaa cattatcaga gttttgttga
    10861 gcagctcggg attaaacagg gtgcagacag tgcattgtct gcccgcaaga aggtgtttaa
    10921 cggaggtgga atgtgagtgt tgttgtttcg gggacgctga aatctcctga tggtgaggcg
    10981 atatcaggcg caaatattac cctgacggcg ctgacagttt caccggatgc gctcagcggc
    11041 accagtgcgt cagcagtgac tcgtgacggc gggtattacg gaatgacgct ggagccgggg
    11101 gagtatgcgg tttctgtgac ggtgaaaggg aagactgctg tctacggacg tgtgcgtatt
    11161 gaggggactg aaagtacggt gacgctcaat atgctgttac gccgcagtct tgttgaggtg
    11221 agcatacccg gagaactgct gacagatttc cggcagatac agaacaatgt ggcggatgac
    11281 cttgccacga ttcgtcgtct gaacgaagac acggcggtga aaaataatca ggcttcacag
    11341 tcgaaggaaa gcgctgctgc cagtgcgaag actgcatcag aaagcgcgaa ggctgcgacc
    11401 agcaaagcga cggagtctgc acaaaaagcc agtgaggctg cagaggcggc gacccgggca
    11461 ggtgagtctg aaaaggcagc ggcagctgac gctaaagagg ccagacgaca tgctgaaacc
    11521 gccagagtgg ctcaggaggc cgccggagac gttcttaaac gtgctgaggc tgctacagtt
    11581 agtgctgaag aagccagacg tatggctgag aatgcacgag gacctaaagg cgatacaggg
    11641 ccgaaaggtg atgctggtcc tcgtggtgaa acaggtccgg cgggtccgcg tggggagaaa
    11701 ggtgagcagg gagagcgcgg acctcagggc ataccgggcc agaaaggtga tacaggagaa
    11761 cggggacctc agggagtgca ggggccagct ggcgcaacag ggccatctgg cccacgtggt
    11821 gcgaccgggc cacagggagc aatggggccg cgtggtgaaa ccggggccag aggcgaaaaa
    11881 ggagacccgg gggggcctca gggaccaaag ggggatgctg gtccgagagg tgagccgggg
    11941 ccgaaaggtg acaccggccc acgtggagag gccggaccac ccggaccaca gggtccggca
    12001 gggcaaaccg gcccgaaagg ggataaaggt gaacccggcg caacaggtcc ggcaggtccc
    12061 gcaggccctc ggggagaaac cggccccgca ggtcctgcgg gtcccgcagg cagtgtcgcc
    12121 agtgttccgg atgccagcac atcacagaaa ggtgttgtac agctgagcag cgataccaac
    12181 agcgatgatg aaacaaaagc ggcaacccca aaggctgtga aagcggtaat ggcagaagtg
    12241 caggcagcaa aaacgaaggc agaagaggca gcaacccggg cagcagtccc cggaccgaag
    12301 ggggacaggg gggaacctgg cgcacctggt gcagtggggc ccgcaggccc acggggaccg
    12361 gctggtgcag ctggtcccaa aggtgatgca ggcccggcag ggccacgagg tgaaaggggt
    12421 ccagcggggg cacagggtgt tccggggccg gttggtccgg caggtcctgc gggtaaaacg
    12481 gggccacgag gtttgcaggg agaaacagga agacagggcc cgacaggtcc acagggcccg
    12541 acaggtgaac ccggaccaca gggaccacaa gggccaaccg gaaggctggt acccggggaa
    12601 atttacagca tcggaacata cattattgcc gcactagccc cgtatataac cgacatcggc
    12661 agaacctatc agccaggaga aactgtatcg ggttcacgcc ttaagcgatg cgccctaatc
    12721 aaagacgaaa acggaaacta tatgaaagct aacaccgatg gcattgacgg caacctatcc
    12781 tcagttccgg gctcatggat ggtgtgtaat gaaattacgt cgacaaatga caatgaaagc
    12841 atcgggctgt tccagcgcgc atactgacag acaggagatg aacatgaatg tggaaaaaat
    12901 aagcaatccg caatgggcag ataaagagca cactgctgtc aattgcatgg tgaagtttga
    12961 gcacattgag caggcggttc ctttcactgc aacagccagc gataccgaag catacgggcg
    13021 ggatatttat gccgcttgtc tgagaggaga ggccggtgaa attgcagaat atgtacagcc
    13081 atccatcagc ccggaaaaag ccagagaact caaaaccgcc gggatcaacg cctggcgtaa
    13141 tgcgatggaa gcggcgaact acacgtttga gcacaatggg cgtaaatggg actacgggaa
    13201 gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg aaagcgggga aactgccgga
    13261 ggcgtttttc tggacggatg cggacaataa tgacgtaccg atggacgctg aaacactgat
    13321 tgctctgagt gctgctgcag agcaggcgat gtttaccaaa gggatggaaa tccacgtccg
    13381 gcagcgcacc atgaagaaag agctggaaaa gctgaccagt gcggatgaga tactggcata
    13441 cagggttggc tgggcacagg aataacagat aaaaatatgg gggaaacatt tctttccctc
    13501 atgaactatt cctcctgtaa atattttatg ttaactgaat agttaatttt ttctgatgtt
    13561 gtaattttgt tcacaaatta cagtgtaagc agatgtgaaa cagcaagtct gactggggta
    13621 agttttttct gatatctgtt ccg
//
LOCUS       DAZFRE010000115        13598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFRE010000115 DAZFRE010000000
VERSION     DAZFRE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.115"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(228..1082)
                     /gene="rclR"
                     /locus_tag="ACHKUI_004183"
     CDS             complement(228..1082)
                     /gene="rclR"
                     /locus_tag="ACHKUI_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6600266.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1309..2634
                     /gene="rclA"
                     /locus_tag="ACHKUI_004184"
     CDS             1309..2634
                     /gene="rclA"
                     /locus_tag="ACHKUI_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6600267.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGRNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            2743..2979
                     /gene="rclB"
                     /locus_tag="ACHKUI_004185"
     CDS             2743..2979
                     /gene="rclB"
                     /locus_tag="ACHKUI_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6600268.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2991..3584
                     /gene="rclC"
                     /locus_tag="ACHKUI_004186"
     CDS             2991..3584
                     /gene="rclC"
                     /locus_tag="ACHKUI_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6600269.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(3744..4613)
                     /locus_tag="ACHKUI_004187"
     CDS             complement(3744..4613)
                     /locus_tag="ACHKUI_004187"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6600270.1"
                     /translation="MEFSVLSNNLKMPMMGFGFFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            4861..5718
                     /locus_tag="ACHKUI_004188"
     CDS             4861..5718
                     /locus_tag="ACHKUI_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6600271.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(5843..10093)
                     /gene="fdeC"
                     /locus_tag="ACHKUI_004189"
     CDS             complement(5843..10093)
                     /gene="fdeC"
                     /locus_tag="ACHKUI_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO6600272.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKTSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGGYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTSLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADNTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRIFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            10659..11510
                     /locus_tag="ACHKUI_004190"
     CDS             10659..11510
                     /locus_tag="ACHKUI_004190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6600273.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLSAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            11537..12526
                     /locus_tag="ACHKUI_004191"
     CDS             11537..12526
                     /locus_tag="ACHKUI_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6600274.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGNDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(12557..13450)
                     /locus_tag="ACHKUI_004192"
     CDS             complement(12557..13450)
                     /locus_tag="ACHKUI_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6600275.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT         3541 a         3347 c         3079 g         3631 t
ORIGIN      
        1 acactgatgg tgcacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgggat
       61 tttatttaat tgaagcaatt tagtttgcat ttttatttaa aaactgagct agagtccatc
      121 aaaaaaaata tataaagccc aaaactaccg ttggaaatag aaatagttga aaggctcaca
      181 actcccggaa tgcggattat ctttgccctg atttctggcg gttttattca gggtgcaagc
      241 tgtctgaccc tttcccgata ttctcccgga gtacaaccaa actcgcggac aaacgccttg
      301 tgaaaagatg attcgctggc atagcctact gactcagcga tcaccacaac agggagcatt
      361 tcccgggaaa acatctgggc cgctatttgt agacgcaact ttgttaatac agccagcggc
      421 gtggttccgg aaacatcacg gaaaagctgg gcaaaacttg cccgggacat gtgggcgatg
      481 ctggccagcg attcgacggt ccaggcgtgt ccaggcattt ccatcatttg ctgtattacc
      541 gcaccaagac gtggatgcag aagcaaactg agaatgtttt tctctgtatt aacctgtgca
      601 atccactcac gcaccgcgag ggtaaagaat gtagcgcaga tctggctaca cagagcatcc
      661 actcccggca ttgccgatct ggattcctgt tgtaaaaacg ggatcgcctc cctcagccag
      721 ttatattcca cgctatggtt cactggtgct acaaacagcg tttccggcaa agacgttaaa
      781 aaataacgcg ccgaatgttg cagccgaaga gtgccacaga caatacaggt cgattcatta
      841 tcgacatgac tcaggcgatg agctgaattt tgtggtagca ggaccacatt tcccgggcgt
      901 aatgtaaaaa tctctcccgt cggcatttcc agcttcgccg ctccttgcgt taacgcatgc
      961 caacgaataa ccgacaattc cccggcacca tgtggaagtt gccagtcact tcctaacacg
     1021 caattcttat cgatcgttcc ttgtggagcg ttaagcatca acaaacggct aagagcatcc
     1081 atatcagact cctgagtggt tttttgatta caaacaccaa caatcttagt caataattaa
     1141 tctaatacca tcaaatagtt aatcatgatt tttgttttgt gattctgatt aagaaaaaga
     1201 aaataccaga cgataagaca aaatatcaag cgttgtgaag aaatgttatt tgctcttttg
     1261 cgtctatagt catgatgtca aatgaacgcg tttcgacagg aaatcatcat gaataaatat
     1321 caggcagtta ttattggttt tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa
     1381 gcaggttggc gtgtggctct catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt
     1441 aatatcggct gtatcccaac caaaacattg gttcatgacg cacagcagca cacagatttt
     1501 gtccgtgcca tacagcgtaa aaatgaagtg gttaattttt tacgtaataa gaattttcat
     1561 aatcttgcgg atatgcccaa tatcgacgtg atcgacggcc aggcagagtt tatcaataat
     1621 catagcctgc gtgttcatcg gcctgggaga aatctggaga ttcatggcga gaaaattttt
     1681 attaataccg gtgcacaagc cgtggttccg ccaattcctg gaattaccac cacgcctggt
     1741 gtatatgaca gcaccggatt acttaatcta aaagaattgc ctgggcattt aggtattttg
     1801 ggcggcggat atattggcgt tgagttcgcc tctatgttcg ctaattttgg cagcaaagta
     1861 accattttag aggcagcttc gctgtttttg cctcgggaag atcgggatat tgctgataat
     1921 atcgcgacga ttttacgcga tcagggtgtc gatataatcc tcaatgccca tgtggagcga
     1981 atcagtcacc atgaaaatca agtgcaagtg catagcgagc acgcccaact ggcggtggat
     2041 gcactgttaa tagcttccgg tcgtcaaccg gctaccgctt cgttacatcc agaaaatgca
     2101 ggtatcgcag taaacgagcg cggggcaatt gtcgttgaca agcaattaca taccaccgca
     2161 gacaatattt gggcgatggg agatgttacc ggcgggctgc aatttactta tatttcactg
     2221 gatgattacc gcattgtgcg tgatgagttg ctgggagaag gcagacgtag tactgatgat
     2281 cggaaaaatg tgccttattc cgtattcatg acaccgccac tgtccagggt tggtatgaca
     2341 gaagaacagg ccagagagag tggtgctgat attcaggtgg tgacattgcc tgtagctgca
     2401 attccgcgtg ccagagtgat gaatgatact cgtggggtat taaaagcgat tgttgataat
     2461 aaaacccaac gtatattagg ggcatcactg ctgtgtgttg actcccacga gatgatcaat
     2521 atagtgaaaa tggtgatgga tgccgggctg ccttatagca tattacgcga tcagatattt
     2581 actcatccgt cgatgagcga atcactcaat gatctatttt cattagtcaa ataaactcaa
     2641 aatcagacgc cagaacaaat attctggcgt ctcagagaaa agaatcttat taattccctg
     2701 ttactttata acttcttaat cacttcattg atggtatttt atatgtttaa aaaatctgtt
     2761 ttatttgcaa cactattatc tggcgttatg gcattttcca ccaatgcaga tgataaaata
     2821 attctgaaac atatcagcgt ctcatcagta tcagcatcac cgacagttct tgagaatgcc
     2881 attgctgata tggccagaaa atataatgct tcatcctgga aagttacatc gatgcgaatt
     2941 gataataatt caaccgcaac agcagtattg tataaataag gatgtttaca atggaaagat
     3001 acctgcacct gttaagtcgg ggagataaaa ttggcctgac attgattcgt ctgagtattg
     3061 cgattgtttt tatgtggatt gggttattaa agtttgtccc ttacgaggca gacagcatta
     3121 caccattcgt cgcaaacagt ccactaatgt cgttctttta tgaacacccg gaagactata
     3181 aacagcatct gactcacgaa ggcgaataca aaccagaagc aagagcatgg caaacggcta
     3241 ataataccta tggtttttcc aacggtcttg gcgtcgtgga ggtgattatt gctctgctgg
     3301 ttttggctaa tcctgtcaat cgctggttag gtttattggg tgggctgatg gcatttacca
     3361 cgccgctggt aacactctca tttttaatca ccaccccgga ggcatgggta cccgcattgg
     3421 gtgacgctca tcatggtttc ccttatttat ccggtgctgg tcgtctggta ttgaaagata
     3481 ctctgatgct ggcaggtgct gtcatgataa tggcagattc ggcgcgggat attcttaaac
     3541 aacgcagtaa tgaatccagt tcaacgttaa aaactgaata ttgataatcc gaacactgtc
     3601 tgttgttcac tttttcaggc gtcaggctcc ctttctgatg atgaagcctg acgccatatt
     3661 accggagata tgacagagtg aaggtcaata tcactcccct attaaaagat tcttgtcgtc
     3721 tcaggtaaaa taagtgatta atatcaatca tgtattttta cagcaagaca accacgaaca
     3781 aactcaggat taaaatgttt taccgattcc ccaacataac ccaaatcaag cgaactaatt
     3841 tgcgccattt ctttctctgt cagtgagaaa tcccaaatag caaaattttc ttcgatacgc
     3901 tgttgatgtg tcgatttcgg aataacgacc actccccgtt gaatattcca acgcagaatg
     3961 acctgagaaa tcgatttttg atgggcatct gcaatactct gaagcagatt attttcaaag
     4021 ggtttatgtc gtccaccacc taacggagcc catgcttcag gctgcacgtt ataatacttc
     4081 atggttgcca gcgcctctgg ttgggcaaaa tagggatgca actcgacctg gttgaccatc
     4141 ggtttaactc ttaccgtttc acaaaagttc gccagaacat gagggtagaa attggaaacc
     4201 ccaatcgctt ttaatttgcc ctcttcatag gcatcttcga gtgcacgcca cgcactaaaa
     4261 taatcgcgca tggcctggtg caataaatag agatcgaagt attctagccc cgattttttt
     4321 aatgatgctt caatacctgc tgctgcaata tcctgattca acatatcctg cacccacaat
     4381 ttagacgtta taaataactc ttctcgggta cataaacctt cagatatggc ttcacgaacg
     4441 gcttcaccga cggcatcttc attaccgtat accgctgcag tgtcgatgag tcgataaccc
     4501 gtgcggatag cattcaacac tgactgtttg cacacgtctt tatcggtaac ctgaaaaaaa
     4561 ccaaatccca tcatcggcat tttcagattg ttacttaata cggaaaattc cactgtcagt
     4621 ccctccatta ggtatgaaca atgagtctac gttaaaacgt aacctcaaat tagtatgtgg
     4681 attttgatat cacttatgca aaaaattcat taataatgta ggactgaaac ctctctattt
     4741 ttggggcaat gaagcagacg ctatcagtgc ttttgccttg ggacttgctg tttttgatac
     4801 acttagggcc caggacataa cgaaaatgtg cgatatgaca aagaattaac ggagtatgtg
     4861 atgatcaggc agaagattct acagcagctc ctggagtgga ttgagtgcaa tcttgagcac
     4921 cctatttcaa tcgaagatat cgcacagaaa tctggctaca gcagacgcaa catccagctt
     4981 ctgttccgaa atttcatgca tgtgcctttg ggagaataca ttcgcaaacg aaggctttgt
     5041 cgtgccgcca ttcttgtccg gctctccgcg aaatctatgc ttgatattgc actctctttg
     5101 cattttgatt cacagcagtc attcagccgc gaatttaaaa agttattcgg ctgctctccc
     5161 cgtgaatacc gccaccgtga ttattgggat ctcgcaaata tcttcccttc ttttttaata
     5221 cgtcaacagc aaaagacgga gtgtagatta gtcaactttc ctgagacacc tatttttggc
     5281 aattcattta aatatgatat tgaagtgtcg aataaattac cggatgaaga agtcaaactg
     5341 cgacgtcatc atttagtcag atgtatgaag aattttaaga cggatatcta tttcgtctcc
     5401 acgttcgaac catcaacaaa atcggtcgat ttgctcacag ttgaaacttt tgccggtacg
     5461 gtatgcaaac aaaccgattc agagatgccg aaagagtgga caattaaccg aggactgtat
     5521 gcctctttcc gctatgaagg tgagtgggag cactaccctg aatgggcgcg aaacctctat
     5581 ctgatggaat tacctgccag ggggttagcc agagtgaacg gcagtgatat tgagcgattt
     5641 tattacaatg aaaacttcgt cgaaattgat agcaataaca ttgtttgcga aatttttatc
     5701 cccgttcgcc cggtttagcc tgtaactcca tcacataaga tttaccctac cctgccaccg
     5761 tctaatcggt gccagggtag gtattcagta aataatggcg ctgcgcatgt caacgccatt
     5821 attatttatt acatcgtcgt ttttatttct cctcagcgcc ttcagtatct gcaggaacgg
     5881 cgttaattgt cacagtcact ggtttcattt cataccctcc atatgtcaaa gagttgaatg
     5941 tgaatgtgta tgtacctggc ttgttggtac taaatatacg cgtgattttc ccgctcgcga
     6001 aatggtcatt tttcgacggc aggaactgat aatccgcctc tgtcacccct tccggagcct
     6061 caatgtcgac ccacatggtt cctcctacgg ggttgacacc cgacatcaga gtaaccgtca
     6121 gtgttgcagt agtcttcccg tccgccacca aagaatctac acgctctgga gatgcagcga
     6181 gcaccgcgtt tttatcatcc gcgacgaatt tagattcggc agttttcgtc gattcctgac
     6241 cgtcggcctg ttccactttc gccgtgagtg tatatgtcgc tgcgacagtg gtcgtggcgg
     6301 tgaaaatagc ctgcccttgc tcattcgttg tcgccgtccc attgggagtc agaactaaat
     6361 tttccgggct ggcagtcagc gtcacttcgc tatctttcag caggttgtta ttggcatcag
     6421 tcaccgtaac tttatacgtc tgtttggttt tgccgtccgc cactgcacgg ttgccaatca
     6481 cctcaatact ggagatggtc gccgtagtgt tatcggccac aaacgttaca gactgcgcat
     6541 tcgaaggctg gtcatttcct gctgacgccg tgatctcata ggttcctgca gtcacggaaa
     6601 ccactttcag ttccgcttta ccctccttgt cggcgttcac catgatatta ccgtctgcaa
     6661 gcggtttgac gccccgaggc agattaaaga cgaccacagc gccaggaaga aggttgccgt
     6721 attgatcctt aaccgttgcc gtcagcgtaa aggcatcttt gccgtttgcc actttttgag
     6781 cagcggcgtc tacctccagg ctcgcctgac cggtactgaa atccgccttg aatttcacct
     6841 tgaccgtctt ctgagagttt ttcaccgagg cctcgatctc aagttcccct gcaaccgttg
     6901 acataagctc aatgtccact ttccctgccg cattggttgt tactgtattc cccgtcttgc
     6961 tggtcacacc ctgcggtaaa gtcagcgtaa cttcttgccc ctgcaacggg ttaccatagc
     7021 tgtccacgac ggtcagggtt atctggttag cagactgtcc attagccagt tggttattaa
     7081 ccttcactgt catatcacga atctcagcct tagatgcgtc acctgcaaca ttcagcacca
     7141 gtggctgagc aacggcattt tggccgttca ctcgcggcat cacagacaac tgacccgcgg
     7201 cagatcccag cgttaaggtc gacacgtaga ccccattacc tttctctgtc cagtttcctg
     7261 ctgaaggacg ctcactcccc gtgctggcgg caccactaaa cactggtgca tctggcttca
     7321 gcccggtgac cggattcccg tacgcatcct tcacggtgaa ggtgagttcc gtcgtcgttt
     7381 taaccgttgg agttttattg tccgcaacaa gcgtagagtt cgctgatgac atctctccgg
     7441 caataacagt cagctgcgcg gcttcggtgg ctgcaggctg gccgttgaag agcggcatga
     7501 cacgaagctc gcccgtcttt ccgcctgtag ttaacgtagc aacataggaa ccgtcacctt
     7561 tttcggtcca actggaaacg gtcgcccctt cagaggcggt ccccgtcaaa cttgccgaca
     7621 acgaaagacc actgatagcg ttgccatgcg catctttcgc caccagcgtt acggttgtgc
     7681 tttcaccggc ttttacgtga tcttctgcga cagagacttt cgactggttt gaagataatg
     7741 catcagccac cacggttact tttgccgcat ttgctgccgc gtcctgccca ttgagcttcg
     7801 gcataacgtc taattcaccc gccgtagtgc cgagagaaat ctgcgcagtc caggtcccgt
     7861 cgccattatc cgtccagcct gatgccgtag aaccagcagc agctgcgccc gataatgacg
     7921 gtgcatccgg attgaggctg gtcacagggt tgtcattagc atcttttgcc gtccagattg
     7981 ccgtaactgt accgccaacc accggtttat caggattcag tgtgatggac gaatgtgctg
     8041 catcaagcgg cccggcaaca aacttcagcg tttgttgtaa tgtgcccagc tgatactgct
     8101 cgctgaaggc acgcacaaca acgtttccgg cacgggtcga agaaaccgtg gcgctgtaac
     8161 cccctggttt tatttccgaa atggcaccaa cggttacacc attagtgtct tgcggaacaa
     8221 gtcgcaggcg gctggcttct cccgtcaccg gattaccctc ggagtccacc gctgtcagtg
     8281 tcagcgtgta ggcttgctga ccatcagcaa ccacatcacc tgacggctcg ttagcgctca
     8341 gggtggagtt tgccacatcc atcatcgtgg cccgcagttc tgcagtgaca gttttgctca
     8401 tgtcatcaac gctaacagta atcgttgcct cacctgactg cgttccggta gtaaagacag
     8461 actgatacac ccctgcttca gtttcggtga actcacccag tgttggcttt gcctgtgatt
     8521 tagtggcctt cagggtacga gtcacaatat ttccagccgg tttgaaggtt agttcagtct
     8581 tgatctgatc tttcatgccc gtgactggct ggccctcggc gtcgcgcaga gacagcacca
     8641 gcggcttttg ctcattaccg ttagcaagca tttgaatacg gctctgaccg tcaagcgtta
     8701 acgccgtacg atcggcgctc ataccagctc cgctaataac cacttctgtc tgcacacgtt
     8761 tcgaggcatt gcctttgtta tcgtaggcaa tcgctgaaat cgcataataa ttgtctttgc
     8821 ctgcctgata agccgggagc gtcacttgcc actgattgcc ctgccccgta atttttccgc
     8881 ctgcggccag caaagacggc gcttcccatt gcacattttt cagaccgtga gttgctttgc
     8941 tgaccacaag ccccagggaa accgtctggc cgcccttgcc ttcaatacgc tcaggcagag
     9001 caatacggat cacttcagat ttgcgatact caagaacgat attgttatta cgctcaacca
     9061 ggtcatagcg gctgcctgcc agcattcgac gctcgcgaat gctgtctgta tcgagttgtt
     9121 tttccagagg ttcgccaatc cgataattaa cttccaggcc aaagcgagtg tcattctcac
     9181 cactcttgcc ctgcttatgc ccggcactca gggtcagaag aggcactggc gtgtaattca
     9241 cttcagcggt aatcgcatgt ggatctttct ggcgtttatc tttaccaaac agcccgactt
     9301 catcgccata atactgttca tacatcaggc ttgcgccaag ctgcggccag gcaggtaaat
     9361 agccctcagc acgaatatcc cagccattcg ccgggcgttc ctgataatcc tcaacatccg
     9421 gcgatgtttt ccagccagaa gcccggatat aaccattggc gctcagtttc aaataatcac
     9481 gccagtattc cgcaccaaca ccaatgcggg tatgactacg ggataaatca tgatcgataa
     9541 aagtattcac ccccgccatc cagtcatttt ctgaaaaatg acgccagcca aaaccaatat
     9601 ttgactgagt acgatcgtcg gtacgatgta ttgccccctg agtgaacaac atatttgtcg
     9661 gtgtatcata aatcggataa agcatttcca gcgaagagtc cttcagcgag aaatttttat
     9721 cgacattcag tttgacgcgc gcagtaccgt atttcccgag ccactcctga atttcctggt
     9781 tagctttagc ggtggccatt ccggtaataa agttacgtgt cgcatcgcta tctggctgac
     9841 tgcttaaaaa tgtcccggca ttagcggcaa gcgacgcgac atttttctcc acgttattat
     9901 cagcagttac cgtagtattt tccatgctca accgtggctg aaccgcatgc tgcgcacgtg
     9961 ctgccattac tggcgtaaag gtgacagcga gtggaaaaag aacctgaaca gagatatttg
    10021 cccacgccac gcagcgggcc agaactgaat aacgaaatcg tggctgttta ttgtctgttt
    10081 tataacgtga cattgagata atttcctgat gaacgaatta acgaacatag gcagacacaa
    10141 ataatggcgt ctgcttgtca gaaaaaccgg ggatttcccg gtttcctttt ccacagaaaa
    10201 ggaaatacct ttatgtaaga aagtacaccg ccacctaata tatccagtgt ctgtagccac
    10261 tcatcaggtg gttgggcgcg ggattatatt tttatttatg ggtaaaaatt tagctttttg
    10321 tgcaagtcat taaatagata cttaagttat atttcttact ctgcgggcag tgaataattc
    10381 attattcaga gtgaataata atgacccgtc tttaattaac tgctattggg agtacgtcga
    10441 agtaagtata tacgccggga taaaccaata tattttttat ggacattcat ttttattcat
    10501 cggactgtaa attccgggga tatcgaactt gcacgactaa tctcaaccct caaacagatt
    10561 tatgaatttt gttcataacc cctgacaaat cctgccgtct aatcgagctc ttccctttac
    10621 gttatgcttc tcatgaacga taacggaagg ataacaccat gcaaaaagta aaactgaaca
    10681 acggtattga aatgcccctg ttgggctttg gtgtatttca gatgaccgat gccgccgaat
    10741 gcgaaagagc ggtcattgac gctattaaca gcggttatcg actgattgac accgccgcct
    10801 cttatcaaaa tgaaatccag gtcgggaacg cactgaaaca aagcggtatc gcccgtaacg
    10861 aactctttgt caccacgaaa ctctggttgc aggataccag ttacgaaggg gccaaagccc
    10921 agttcgaacg ctctctgaat cgactgcaac ttgattacgt tgacctgtac ttgattcacc
    10981 agccatatgg cgatgtccac ggagcctggc gtgccatgga agagctgcaa caggccggta
    11041 aaattcgcgc tattggcgtc agtaacttcc atcctgaccg actggcagac cttatcgctt
    11101 ttaataacgt cgttccggcg gtaaaccagg ttgaagttaa ccctttcaat cagcagctac
    11161 aggccgttcc ctggatgcaa agtcgtggca ttcagccaga agcctgggca ccgtttgcgg
    11221 aagggaaaaa tggtctgttc cagcatcctg ttctctcggc aattggcgag aagtatggca
    11281 aaagcgtggg ccaggtagtt ctgcgctgga tctaccaacg aggcattgtt tcactggcaa
    11341 aatcagtgcg aaaagaacgc atggaagaga acatcaacat tctcgatttt gaactcagcc
    11401 ctgaagatat gctgcaaatt accgccctgg ataccgcaac cagcgcgttc ttctctcacc
    11461 gcgatcccgc aatggtggaa tggctgactg gccgcaaact tgatgtttaa gccgcgataa
    11521 aagaggtatt cattcaatgc aaaaacgtta tctgggtaaa tccggactcg aagtctccgc
    11581 ccttgggctc ggttgcatgg gcttaagcca cggctacggc ccggcgaccg atacccgtca
    11641 ggctatcgaa cttattcgtg ctgcggttga acgtggcgtc accttcttcg ataccgccga
    11701 agtgtatggt ccgtatctga atgaagaagt ggtcggcgaa gccttaaaac cgtttcgtga
    11761 ccgcgtggta attgccacta aattcggttt taccttcggc aatgataata agcagcagat
    11821 tttaaacagc cgtccggaac atatccgtga agccgttgaa ggatcattac gtcgtctgaa
    11881 aacagatgtt attgacctgc tgtatcaaca ccgtgttgat ccagatgttc ctattgaaga
    11941 tgttgcagga acggtgaaag acctcatcgc agaaggtaag gtcaaacatt tcggcctgtc
    12001 tgaagcagga gcacaaacca ttcgtcgtgc acatgcggta caacctgtca ccgccctgca
    12061 aagcgaatat tccatgtggt ggcgtgagcc tgaacaggaa attctgccgt tgcttgagga
    12121 actgggaatt ggttttgtcc ccttcagccc actaggtaaa ggtttcctga caggggcgat
    12181 taagccggga accactttcg gcaaagatga ttaccgcagc accgtaccgc gtttcgcagc
    12241 acaagccatt gaagccaatg aaaaactggt caccttgtta ggtgaactgg ctgcggagaa
    12301 aggggtaacg tctgcacaaa tcgcactggc atggctatta gcacaaaagc cgtggattgt
    12361 tcccatccct ggcaccacta aactgcaccg gctggaagaa aacctggccg ctgctgacat
    12421 tgttctttcg cagaaagata cgcagcagat aagcgaggcg ctggaaacaa ttaaaatcgt
    12481 cggtgaacgt tactcgcctg aacatcaggc acgcgtaggc cgctaatacc caaacgggtc
    12541 caggataaag gacccgttat tcggtgtagc gaagcgcatc gatcaacaac gcaaaagctg
    12601 gcggatgctg cttgcggctg gggtagtaaa gataataccc aggaaaagtt ggacaccact
    12661 cctgtaacac ctgaataagc ttgcctgact ctatatactc ctgaaccatg tcttcaggga
    12721 cacaagcgat cccaaaccct gataacgcgg catcaattct ttctggcaat ggactacacg
    12781 tcagttgccc atcaacctta acgcgaagcg gtttcccttc tcgctcgaac tcccagtggt
    12841 aaagcccacc ggcagttggc aaacgcatgt tgatgcagcg atgattttgt agctcgtgag
    12901 gcgtttcagg cgcagggttt gcagcaaaat acgccggagc tcccaccaca gccatccgca
    12961 tatctggtcc aatcctgact gcaaccatat ctttatcaac attttcaccc agtcgtatcc
    13021 cggcatcaaa acggccttca acaatatcga caaaaccgtt atccaccacc agttcgacat
    13081 tgatttcggg atactctctg agaaaaggtt ttagcttcgg ccagaccaga cttcgcgcgg
    13141 catgttcccc ggcagataac cgaatattcc cggagggcgt gccgttcaat tgaataagtg
    13201 attccagttc ctgttcaagc tcgtcaatac gcggttcaag gcaggcaatg attctctccc
    13261 ccgcttctgt gggagccaca ctgcgggtag tccgtgtcag gaggcggata ttgagccttt
    13321 cctccagagc cttcattgca tggctaagtg cagactgaga gacgccgagt ttgcccgctg
    13381 ctttggtaaa acttcgctct cttgccacca caagaaagat ttgcagttcg ttgaagtttt
    13441 ctttgagcat tggcgatttc cttgtagaag catcctcttc acagacactc atgaaggggg
    13501 gcatagatgc tattatttta accacattat tagtaacaat gataatctgg ctgtatcgaa
    13561 aataattcta aactttcagg agatttatta tgaataaa
//
LOCUS       DAZFRE010000116        13566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFRE010000116 DAZFRE010000000
VERSION     DAZFRE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.116"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHKUI_004193"
     CDS             complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHKUI_004193"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6600276.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHKUI_004194"
     CDS             complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHKUI_004194"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6600277.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHKUI_004195"
     CDS             complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHKUI_004195"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6600278.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGITAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHKUI_004196"
     CDS             complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHKUI_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6600279.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHKUI_004197"
     CDS             complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHKUI_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6600280.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHKUI_004198"
                     /pseudo
     CDS             complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHKUI_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5173..6483)
                     /locus_tag="ACHKUI_004199"
     CDS             complement(5173..6483)
                     /locus_tag="ACHKUI_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO6600281.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTISIDL
                     QKIDSSTLGLNGFSVSGQSLNVSDSITQITGAAGTKPVGVDFTAVAKDLTTATGKTVD
                     VSSLTLHNTLDAKGAATSQFVVQSGNDFYSASINHTDGKVTLNKADVEYTDTDNGLTT
                     AATQKDQLIKVAADSDGSAAGYVTFQGKNYATTVSTALDDNTAAKATDNKVVVELSTA
                     KPTAQFSGASSADPLALLDKAIAQVDTFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6731..8146
                     /gene="fliD"
                     /locus_tag="ACHKUI_004200"
     CDS             6731..8146
                     /gene="fliD"
                     /locus_tag="ACHKUI_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6600282.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSDNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            8162..8572
                     /gene="fliS"
                     /locus_tag="ACHKUI_004201"
     CDS             8162..8572
                     /gene="fliS"
                     /locus_tag="ACHKUI_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6600283.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKASLLSPSLIQDPV"
     gene            8572..8937
                     /gene="fliT"
                     /locus_tag="ACHKUI_004202"
     CDS             8572..8937
                     /gene="fliT"
                     /locus_tag="ACHKUI_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6600284.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLLLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9015..10502
                     /gene="amyA"
                     /locus_tag="ACHKUI_004203"
     CDS             9015..10502
                     /gene="amyA"
                     /locus_tag="ACHKUI_004203"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6600285.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10536..10949)
                     /gene="yedD"
                     /locus_tag="ACHKUI_004204"
     CDS             complement(10536..10949)
                     /gene="yedD"
                     /locus_tag="ACHKUI_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6600286.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11136..12341
                     /gene="yedE"
                     /locus_tag="ACHKUI_004205"
     CDS             11136..12341
                     /gene="yedE"
                     /locus_tag="ACHKUI_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6600287.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            12338..12571
                     /gene="yedF"
                     /locus_tag="ACHKUI_004206"
     CDS             12338..12571
                     /gene="yedF"
                     /locus_tag="ACHKUI_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6600288.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12680..13348
                     /locus_tag="ACHKUI_004207"
     CDS             12680..13348
                     /locus_tag="ACHKUI_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6600289.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGGWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFIAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3360 a         3488 c         3357 g         3361 t
ORIGIN      
        1 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
       61 gttttatcac gtttgaaatg gcgtcccgcc ccgactggtg aatcgtcggg gctgattttt
      121 tcttattatt gcagcagaaa cttctccagg aactggcggg tgcgaggctg ctgaggatca
      181 gcaaataacg cttttgctac cccctgctcg actatccgcc cctggtccat aaaaatcgcc
      241 cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt
      301 tcctgcgcca gctgacgaat ggtgttcagg acttcgccca ccagctctgg atccagcgcc
      361 gacgttggct catcaaacaa aatcacttca ggacgcattg ccagagcacg cgcaatcgca
      421 acgcgctgct gttgaccgcc agacaaacga cgtggataac tggtttcttt accggccagc
      481 ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
      541 acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa cagattaaag
      601 ttctggaaga caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
      661 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tggttcccgc ttccggttgt
      721 tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataata
      781 gccactactt cgccaggctt cacctcaagg tcgataccat gcagcaccgt ctgaccgtgg
      841 aattttttca ccaggttctt aacttcaatg gcactcattt gggttccctc tcctggcgat
      901 taagttgatt ctcaaaatgg ttctgcaacg tcgataacac agtcgccata atccagtaga
      961 tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg
     1021 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc
     1081 tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
     1141 gacgcatggt ctgccatggc gtcataccaa tactggctgc agcttcccac tgacctttat
     1201 caatagaaga aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcagcgaca
     1261 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgtggc agtccgtaat
     1321 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
     1381 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca
     1441 gtaagccaaa aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca
     1501 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
     1561 aacgtgggga tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg
     1621 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc
     1681 ggtatacaca ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat
     1741 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
     1801 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tggaggttaa ccactttcgg
     1861 caattgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
     1921 gtgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgacg cgaccaccac
     1981 cgacgaaata ttaactgccc cttcacactg ttgcgcaatt tccagcgcac tctccacata
     2041 acccagcgcg cccaaagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
     2101 tgcttcgact cgcgtcgcca gctcttccag ttgggtattg ggatcggtca gtgcgtcgca
     2161 catttcaatc tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt
     2221 ttctgcggtt atgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc
     2281 cgcaactgcg gcagtctggc gcacatggtt agactggatc gccccagcag taatcagcgt
     2341 atcggcacct tcacgcagag catctgccgc gagaaattcc agcttacgta atttattgcc
     2401 gcccattgcc atgggagtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc
     2461 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca gccgtggaaa
     2521 acgggttaaa ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat
     2581 tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat
     2641 tatgctgatt atttggtcac atcagcacca aaccattttt cggaaagggc ttgcagagtg
     2701 ccgtcttttt gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct
     2761 ttacgcaacg ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta
     2821 tcgttggttt tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata
     2881 cggcctacgc gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgaca
     2941 ccctgaacat tctgacgcag ccactcttca tagttggtgc ccagaccgac acccactttt
     3001 ttgcctttca gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc
     3061 tgaataccag aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa
     3121 atggtgacct gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg
     3181 tcccatttag tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca
     3241 aattccactt caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa
     3301 gttccttcca gccctaccag cagcgtgccg cgctctttga ctttattaag cagaccttca
     3361 tctgcaaaac ttttaacgct catacccgca accagcgcca cggccatcac acccatcaat
     3421 gcctgacgtc ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc
     3481 agtgtagagc cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa
     3541 tatatatcag aagcaggttg gctggaggat ttctgcacaa gtccggtaca cgtttgacgc
     3601 tgatagtgtt gatacttccg taacgcgatg cggtaattgt tggtgctggt ggagggcagc
     3661 cacggggcga ggatttcatc aaggaaaccg tcctgcagct ggtcgaggga aatattttgc
     3721 tcgtgtagat gattccccaa acggcgcaga cgaacgacat attcacgcac cgtaccaggg
     3781 ctcatttcag tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc
     3841 tgcgttttgc aatgtaaatc accgcaaaat cggcacaatg ccgcccatga ttgttgttcc
     3901 acttgccagc cattttcatc aagcagcgta tccaggcggg aaatctcgat tttattcact
     3961 attttgccgt cgcgaactaa ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg
     4021 gtctggctgt gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc
     4081 accatcatta agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac
     4141 ttacccagtt tagtgcgtaa ccgtttaata gcctggctgt gtaactgact gacccgcgat
     4201 tccccgacct ccagcaccgc accaatctct ttgagattca gctcttcctg ataatagaga
     4261 gttaatacca gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctggcgc
     4321 agattactgt ccagcagtta ttgtagcggg ttttctcgct gatgatcatc agtaaccagt
     4381 tcgatgctat cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg
     4441 gtgtcgagca acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca
     4501 gtttccgtgg cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca
     4561 cgcgcgttgc gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata
     4621 gcgccacgta tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag
     4681 cgttcgacgg cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg
     4741 ctcgcgggca gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc
     4801 tgccacagcg agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac
     4861 agccctgcgt tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcggg
     4921 cgattagtgg gtgaaatgag gggttatttg ggggttaccg gtaaattgcg ggcagaaaaa
     4981 aaccccgccg ttggcgggga agcacgttgc tgacaaatta cgcttcatgt tgccggatgc
     5041 ggcgtaacgc cttatccggc ctacaaaaat ctgcaaatta aataaattgc aattcacctt
     5101 gtaggcctga taagcgcagc gcatcaggca atttggcgtt gccgccagtc tcagttaatc
     5161 aggttacgac gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt
     5221 tgccagtacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc
     5281 ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tggtggtgtt
     5341 gttcaggttg gttactgcgg aatccagacg gttttgcacc gcaccgaggg aggagcggaa
     5401 agtatcaacc tgtgcaatag ctttgtctaa aagtgccagt ggatcagcag aagaagcccc
     5461 tgagaactgt gcagtcggtt ttgctgttga taattcaaca acaactttat tatctgttgc
     5521 ttttgccgca gtattatcat caagtgccgt tgaaaccgtt gtagcgtagt ttttaccttg
     5581 gaatgttaca tatcccgcag ccgagccgtc agagtcagcg gcaactttaa tcagttgatc
     5641 tttctgagta gccgcagtcg ttagtccatt atcggtgtct gtgtattcga catcggcttt
     5701 attcaacgtg actttgccgt ctgtatgatt aatcgacgcg gagtagaaat cattgccgga
     5761 ttgaacgacg aactgtgatg tagcagcccc tttcgcatcc agagtgttgt gtaacgtcag
     5821 gctagaaaca tcgactgttt tacctgtcgc agtagtcaga tctttcgcaa cagcagtgaa
     5881 atcaacacca acaggttttg tcccggcggc accggtaatt tgagtaatgg aatcactaac
     5941 gttaagtgac tgacccgaaa cggagaaacc gttcagtcct aatgtagaag agtcaatctt
     6001 ctgcaaatca atgctaatag tctgcccatc attcgcgcca acctgaatct tcatcgaacc
     6061 atctttcgcc agcacattca cgccgttgaa ctgggtctga ccggaaacgc gatcaatttc
     6121 atccagacgg gatttgattt cgtcctgaat agaagacagg tcagaatcag agttagtccc
     6181 ggtcgtcgcc tgaacggtca actcacgcac acgctgcaag ttgttgttga tttcagacag
     6241 tgcgccttcc gtggtctgcg ccagagaaat accgtcgttg gcgttacgtg cggcctgagt
     6301 cagacctttg atgttagaag tgaagcggtt agcaatcgcc tggcccgcag cgtcatcttt
     6361 agcgctgtta atacgcagac cagaagagag gcgctcgata gaagtcgaca gcgcagactg
     6421 gtttttgttg atgttgttct gagtgatcag cgagaggctg ttggtattaa tgacttgtgc
     6481 cataattcat tttcctgttt tcaagtctac ggtttaagta ttgggcttcc acccttcggc
     6541 ttcgtcgccg tcaaccctgt tatcgtctgg tcactcacaa cctttagaat ttttttctag
     6601 cggtcgaaat actccccttc ctcgttgctg ttcgtgccat taaggcaaaa aataaatcgt
     6661 aaactttacc ccgcttacgc cgataacccc ggtattcgtt ctatacacgt cgcatgatta
     6721 aggaaactat atggctagta tttcaacgct tggggttggg tcggggctgg acttaagcag
     6781 catccttgac agtctggagg cggcagaaaa atctaccctg acgccgatat caaaacagca
     6841 aagctcttac accgcgaaac tgagcgccta cgggacgctg aaaagtgcac tagagagttt
     6901 ccagacagcc aacacagcac tgaataaagc cgatttattt accgccacct caaccaccag
     6961 cagttcatcg gctttcagcg ccaccaccac gggcagcgcg attgctggga aatacaccat
     7021 tagcgttagc caactggcgc aggcacagac gctgacaaca aaaaatagtc aaaaagacag
     7081 taaagccgcc atcgccacca gcgatagcgt actgaccatc caacaaggcg gcggtaaaga
     7141 tcctgtcacg attgatatca gcgcgggtaa ctcgtcactt tccggcattc gcgatgccat
     7201 taacaacgcc aaagctggtg tcagcgccag tatcatcaat gtgggaaacg gcgaatatcg
     7261 tctgtccatc accgctaacg atacgggcag cgataacgct atgaaactta gcgtcagcgg
     7321 cgacagcgcc ctggaaagtt ttatgggtta caacggaacg ccgggtgata gcagcaatgg
     7381 catgattgaa agcgtcaccg cgcaaaacgc caaactgaca gtcaataacg ttgagattga
     7441 aaacagcagt aacactatca gcgactcact ggaagatatc accctcaacc tgaatgacgt
     7501 caccaccggt aatcagacac tgaccatcag taaagacaca tccaaagctg aaaatgcggt
     7561 taaagcgtgg gtggatgcct ataacacatt gcaggataca ttcagcagct taaccaaata
     7621 caccgctgtc gatgccggag ctgaaagtca ggactccagt aatggcgcat tactcggtga
     7681 ctccacactg cgtaccatcc agactcagct taaaaccttg ctcgccaata ctcacagcag
     7741 ttcaaactat aaaacgctgg cacagattgg cattaccagc gatgccagta ccggcaagct
     7801 ggaaatcgcg actgacaaat tgcaaacggc gctaaaaaat gatgccgctg gtatcggtga
     7861 aatgtttata ggtgacggca aaagtactgg ggtaaccacc ggcattagta gcaacctgac
     7921 cagttggctc tcttccaccg ggataatcca ggcagcaaaa gatggcgtca gcaaaacgct
     7981 gaataatttg accgaccagt acaacgcagc cagtgaacgt atcgatacct taatgacgcg
     8041 ctataaagcc cagttcaccc aattagatgt gttaatgaac tcgctgaact ccactagtag
     8101 ctatctgaca cagcagttcg acacctcaaa tagcaactcg aaataatctt caggagagac
     8161 tatgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcgccgtaat
     8221 gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt
     8281 tagagcgcgc ctgtttatgc aggacaacaa tcagcaaggc aaaggtgtct ctttgtcaaa
     8341 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga
     8401 attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa
     8461 tttacgcaac gatgtctccg cagtcgaaga ggtggaagca ttaatgcgta atattgccga
     8521 tgcctggaaa gcgtctttac tctccccttc tttgattcag gacccagtct gatgaacaat
     8581 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
     8641 ctggcgacag aagaacaatg ggacgaactc atcgccagcg aaatggcgta tgtgaatgcg
     8701 gtgcaggaga ttgcacatct gactgaagag attgacccat ccaccacgat gcaggagcag
     8761 ctccgcccga tgctgctcct gattctcgac aacgaaagca aggtaaagca gttattacag
     8821 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
     8881 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
     8941 ttaatgagcc gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg
     9001 aatggagtcg aagaatgcgt aatcccactc tgttacaatg ttttcactgg tattacccgg
     9061 aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta
     9121 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct
     9181 acgactccta tgatttattt gatttaggtg agtttgatca gaaaggcagc atccctacta
     9241 aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg
     9301 cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
     9361 gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg
     9421 aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg
     9481 atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta
     9541 aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta
     9601 atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga
     9661 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg
     9721 cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta caggaagttg
     9781 cgccaaagcc gctgtttatt gtggctgaat actggtcgca tgaagtcgat aaactgcaaa
     9841 cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat
     9901 tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc
     9961 tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt
    10021 tgcaagccct cgaagccccc gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
    10081 tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattatg
    10141 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
    10201 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacgtta tttttcgacc
    10261 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
    10321 tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
    10381 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgaccgac gaaaacggcg
    10441 aagcaacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    10501 aaattcatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    10561 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    10621 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    10681 acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat cacccgctgc
    10741 cactgacggc aatcgagggt atcgccctct ttagtgacaa tgaggctgcc tatcgcttcc
    10801 ggactgacca acgcacgttg cggccctttg gtctgccagt aacccgtcag ccagtccggc
    10861 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    10921 agcgcacctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    10981 gtggcattaa agccctgatg tcgccagcct ttccaggctc agagagaaat ttgatctaac
    11041 cattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcgactc
    11101 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    11161 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    11221 cttactattt tggcatcact ggcacatttt gggcagtcac gggtgaattt acccgttggg
    11281 gcggtcagct tttgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    11341 tccatctgga aggatcgcca ttaacccgca tcgacggaat gatgatcctc ggtatgtttg
    11401 gcggctgttt tgccgcagcg ctgtgggcca acaatgtcaa actgcgcatg ccgcgcagcc
    11461 gtatccgcat tatgcaggcc atcattggcg gtattatcgc cggttttggc gcacgtctgg
    11521 caatgggctg taacctggcg gcgttcttta ccggaattcc acagttctcg ctgcacgcct
    11581 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
    11641 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc tgcctcaccg ttgacgcaaa
    11701 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
    11761 gctgggcgct gctgacggcg atgaaccagc ccaaactggg gctggcaatg ctgtttggcg
    11821 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    11881 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtaagtg
    11941 ccatcgggat cttcagttac gtacagttag gcgttgaacc caaaatcatg tgggcgggac
    12001 caaacgcggt aattggtggt ttactgtttg gttttggcat cgtgctggca ggcggctgcg
    12061 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    12121 gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
    12181 ccaccgactg ggacaaaatc aacctcctga aaacctttgg cccgatgggc ggcctgctgg
    12241 tgacatattt gctgttgttt gcagctctaa tgttgattat cggctgggaa aaacgcttct
    12301 tccgccgtgc ggcaccgcag attgctaagg agatcgcatg aaaaatatcg ttcctgatta
    12361 ccgtcttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
    12421 gccgcagtta aaaaaagggg agatcctgga agtggtgagc gactgtccgc agtcgatcaa
    12481 taatattcca ctggatgcgc gtaatcacgg ctatacggtg ctggatattc agcaagacgg
    12541 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    12601 gaacatcatt acctctctat cacatagatt ttacccgccc cctctcctat tcttttcagt
    12661 gtgacctcag gaggtttcta tgtgtggacg ctttgctcaa tcccaaacgc gtgaagatta
    12721 ccttgcgctt ctcgcggaag atattgaacg cgatattccc tacgatcccg aacctattgg
    12781 cagatacaac gtcgcgccgg gaaccaaagt ccttctgctc agtgaacgtg atgaacacct
    12841 tcatctggat ccggttttct ggggatatgc tcccggaggg tgggataaac cgccgctgat
    12901 taacgcccgc gtagaaactg ccgcaaccag tcgcatgttt aaaccgctct ggcaacatgg
    12961 tcgggcaatc tgctttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaca
    13021 gccttatttt atctatcgcg cagatggaca acctgttttt atagccgcga taggtagcac
    13081 accgtttgag cgcggtgacg aagccgaagg atttttaatt gtcactgcag cagcagacca
    13141 gggtctggta gatattcatg accgccgccc gctggtactg tcgccagaag ctgcacggga
    13201 atggatgcgg caagagatta gcggtaaaga agcctctgaa attgctgcca gtggctgtgt
    13261 tccagcaaac cagttcacct ggcacccggt atcgtgcgcg gtgggtaatg ttaaaaacca
    13321 gggcgcggag ttaattcaac ctgtttgata agcctggaga ttatctacac ctggatggac
    13381 atcattgtac tatgccccgt acatgcccca tcacatcctc aacatcaaag tattagagcc
    13441 ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa aaatagaact
    13501 aatatcaagc ggcgctatat tgcgtattaa ataaaaccac ctcaattaca ttaataaatc
    13561 taaaca
//
LOCUS       DAZFRE010000117        13296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFRE010000117 DAZFRE010000000
VERSION     DAZFRE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.117"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            106..1044
                     /gene="tdcA"
                     /locus_tag="ACHKUI_004208"
     CDS             106..1044
                     /gene="tdcA"
                     /locus_tag="ACHKUI_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6600290.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            1143..2132
                     /gene="tdcB"
                     /locus_tag="ACHKUI_004209"
     CDS             1143..2132
                     /gene="tdcB"
                     /locus_tag="ACHKUI_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6600291.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTICVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2154..3485
                     /gene="tdcC"
                     /locus_tag="ACHKUI_004210"
     CDS             2154..3485
                     /gene="tdcC"
                     /locus_tag="ACHKUI_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6600292.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3511..4719
                     /gene="tdcD"
                     /locus_tag="ACHKUI_004211"
     CDS             3511..4719
                     /gene="tdcD"
                     /locus_tag="ACHKUI_004211"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6600293.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVVLIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4753..7047
                     /gene="tdcE"
                     /locus_tag="ACHKUI_004212"
     CDS             4753..7047
                     /gene="tdcE"
                     /locus_tag="ACHKUI_004212"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6600294.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7061..7450
                     /locus_tag="ACHKUI_004213"
     CDS             7061..7450
                     /locus_tag="ACHKUI_004213"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6600295.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7522..8886
                     /gene="tdcG"
                     /locus_tag="ACHKUI_004214"
     CDS             7522..8886
                     /gene="tdcG"
                     /locus_tag="ACHKUI_004214"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6600296.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9164..10494
                     /locus_tag="ACHKUI_004215"
                     /pseudo
     CDS             9164..10494
                     /locus_tag="ACHKUI_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10522..11832
                     /gene="cyuA"
                     /locus_tag="ACHKUI_004216"
     CDS             10522..11832
                     /gene="cyuA"
                     /locus_tag="ACHKUI_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6600297.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11893..12057)
                     /gene="yhaL"
                     /locus_tag="ACHKUI_004217"
     CDS             complement(11893..12057)
                     /gene="yhaL"
                     /locus_tag="ACHKUI_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6600298.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12080..12781)
                     /locus_tag="ACHKUI_004218"
     CDS             complement(12080..12781)
                     /locus_tag="ACHKUI_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6600299.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKSESANFQLHGPRAYLQSIHGKFHALTHHKEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12886..>13296
                     /gene="yhaJ"
                     /locus_tag="ACHKUI_004219"
     CDS             12886..>13296
                     /gene="yhaJ"
                     /locus_tag="ACHKUI_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6600300.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
BASE COUNT         3166 a         3106 c         3556 g         3468 t
ORIGIN      
        1 gccatggaaa gtattgaaag ttaatttgtg agtggtcgca catatcctgt tcatttcatt
       61 ttgatacact tcatgccgtc aatgaggtaa ttaacgtagg tcgttatgag cactattctt
      121 cttccgaaaa cgcagcacct ggtagtcttt caggaagtta ttagaagtgg ttctatcggc
      181 tcggctgcaa aagagttagg gttaactcaa ccggccgtca gtaaaatcat taacgatatt
      241 gaggattatt ttggtgtgga gttagtggtg cggaaaaata ccggtgtaac attaacacct
      301 gccggtcaat tgttactctc ccgttccgaa tccattaccc gtgaaatgaa aaatatggtt
      361 aatgagataa gcggtatgtc ttctgaggcg gtggtggaag tctcatttgg ttttccttca
      421 ttgattggtt ttacttttat gtcagggatg atcaacaagt tcaaagaggt gttcccgaaa
      481 gcgcaggttt ctatgtatga agcgcaactg tcttcgttct taccggcaat ccgcgacggc
      541 cgactggatt ttgcgattgg tacgttaagc gcagaaatga agcttcagga tttacatgtt
      601 gagccgctgt tcgagtccga gtttgtgctg gtagccagta agtcccgaac atgcaccggc
      661 accaccacgc tggagtcgtt gaagaacgaa cagtgggtgt tgccacaaac caatatgggg
      721 tactacagcg aactacttac tacgttacaa agaaatggca tcagtattga aaacatcgtt
      781 aaaaccgact cagtcgtgac aatttataat cttgttctca atgctgattt cttaactgta
      841 attccttgtg atatgacatc accttttggt tctaatcaat ttattactat tccggttgaa
      901 gaaacattac ctgtggcaca atatgccgcg gtatggtcga aaaattatcg tattaaaaaa
      961 gcagcatcgg ttttggtgga attagccaaa gagtattcat cttataatgg gtgtagacga
     1021 aggcaattaa ttgaagttga ttagttattt gttttaattt aaacataaat aatccacctg
     1081 tctgttttgc aggtgtcggt tacggttacc tacatattta attcaggcta agaggtttta
     1141 taatgcatat tacatacgat ctcccggttg ctattgatga catcattgaa gcaaaacaac
     1201 gactggctgg gcgaatttat aaaacaggta tgcctcgctc caactatttt agtgaacgtt
     1261 gcaaaggtga aatattcctc aaattcgaaa acatgcagcg taccggttca tttaaaattc
     1321 gtggcgcatt taataaatta agttcactga ctgatgcgga aaaacgtaaa ggtgtagtgg
     1381 cctgctctgc gggcaaccat gcgcaagggg tttcgctctc ctgcgcgatg ctgggtattg
     1441 acggtaaagt ggtgatgcca aaaggtgcgc cgaaatccaa agtcgcggca acgtgcgact
     1501 actccgcaga agtcgttttg catggtgata acttcaacga cactattgct aaagtgagcg
     1561 aaattgtcga aatggaaggc cgtattttta tcccacctta cgatgatccg aaagtgattg
     1621 ctggccaggg aacgattggt ctggaaatta tggaagatct ctatgatgtc gataacgtga
     1681 ttgtgccaat tggcggcggc ggtttaattg caggtattgc ggtggcaatt aaatctatca
     1741 acccgaccat ctgtgttatt ggcgtacagt ctgaaaacgt tcacggcatg gcggcttctt
     1801 tccactccgg agaaataact acgcaccgaa ctaccggcac cctggcggat ggttgtgatg
     1861 tctcccgccc gggtaattta acttacgaaa tcgttcgtga attagtcgat gacatcgtgc
     1921 tggtcagcga agacgaaatc agaaacagta tgattgcctt aattcagcgc aataaagtcg
     1981 tcaccgaagg cgcaggcgct ctggcatgtg ctgcattatt aagcggtaaa ttagaccaat
     2041 atattcaaaa cagaaaaacc gtcagtatta tttccggcgg caatatcgat ctttctcgcg
     2101 tctctcaaat caccggtttc gttgacgctt aattaattcg ttgaggatag gatatgagta
     2161 cttcagatag cattgtatcc agccagacaa aacaatcgtc ctggcgtaaa tcagatacca
     2221 catggacgtt aggcttgttt ggtacggcaa tcggcgccgg ggtgctgttc ttccctatcc
     2281 gcgcaggttt tggcggactg atcccgattc ttctgatgtt ggtattagca taccccatcg
     2341 cgttttattg ccaccgggca ctggcgcgtc tgtgtctttc tggctctaac ccttccggca
     2401 acattacgga aacggtggaa gagcattttg gtaaaactgg cggcgtggtt atcacgttcc
     2461 tgtacttctt cgcgatttgc ccactgttgt ggatttatgg cgttactatt accaatacct
     2521 ttatgacgtt ctgggaaaac cagctcggct ttgcaccgct gaatcgcggc tttgtggcgc
     2581 tgttcctgtt gctgctgatg gctttcgtca tctggtttgg taaggatctg atggttaaag
     2641 tgatgagcta tctggtatgg ccgtttatcg ccagcctggt gctgatttct ctttcgctga
     2701 tcccttactg gaactctgca gttatcgacc aggttgacct cggttcgctg tcgttaactg
     2761 gtcatgacgg tatcctgatc accgtctggc tggggatttc catcatggtt ttctccttta
     2821 acttctcgcc aatcgtctct tccttcgtgg tttctaaacg tgaagagtac gagaaagatt
     2881 ttggtcgcga cttcaccgag cgtaaatgtt cacaaatcat ctctcgcgcc agtatgctga
     2941 tggttgcagt ggtgatgttc tttgccttta gctgcctgtt tacgctgtct ccggcgaata
     3001 tggctgaagc gaaagcgcag aatattccag tgctttctta tctggctaac cactttgcgt
     3061 ccatgaccgg taccaaaaca acgtttgcga ttacactgga atatgccgct tccatcatcg
     3121 cactcgtggc tattttcaaa tctttcttcg gtcactatct ggggacgctg gaagggctga
     3181 atggtttgat tctgaagttc ggttataaag gtgacaaaac caaagtgtcg ctgggtaaac
     3241 tgaatactat cagcatgatt ttcattatgg gctccacctg ggttgttgcc tacgccaacc
     3301 cgaacatcct cgacctgatt gaagccatgg gcgcaccgat tatcgcatca ctgctgtgcc
     3361 tgttgccgat gtatgccatc cgtaaagcgc cgtctctggc gaaataccgt ggtcgtctgg
     3421 ataacgtgtt tgttaccgtg attggtctgc tgaccatcct gaacatcgta tacaaactgt
     3481 tttaatccgt aactcaggat gagaaaagag atgaatgaat ttccggttgt tttggttatt
     3541 aactgtggtt cgtcttcgat taagttttcc gtactcaatg ccagcgactg tgaagtatta
     3601 atgtcaggta ttgccgacgg tattaactcg gaaaatgcat tcttatccgt aaatggggga
     3661 gagccagcac cgctggctca ccacagctac gaaggtgcat tgaaggcaat tgcatttgaa
     3721 ctggaaaaac ggaatttaaa tgacagtgtg gtcttaattg gccaccgcat cgctcacggc
     3781 ggcagtattt ttaccgagtc cgccattatt accgatgaag tcattgataa tatccgtcgc
     3841 gtttctccac tggcacccct gcataattac gccaatttaa gtggtattga atcggcgcag
     3901 caattattcc cgggcgtaac tcaggtggcg gtatttgata ccagtttcca ccagacgatg
     3961 gctccggaag cttatttata cggcctgccg tggaaatatt atgaagagtt aggtgtacgc
     4021 cgttatggtt tccacggcac gtcgcaccgc tatgtttccc agcgcgcgca ttctctgctg
     4081 aatctggcgg aagatgactc cggcctggtt gtggcgcatc ttggcaatgg cgcgtcaatc
     4141 tgcgcggttc gcaacggtca gagtgttgac acttccatgg gaatgacgcc gctggaaggc
     4201 ttgatgatgg gtacccgcag tggtgatgtc gacttcgggg cgatgtcctg ggtcgccagc
     4261 caaaccaacc agagcctggg ggacctggaa cgcgtagtga ataaagagtc gggattatta
     4321 ggtatttccg gcctttcttc ggatttacgt gttctggaaa aagcctggca tgaaggtcac
     4381 gaacgcgcgc aactggcaat taaaaccttt gttcaccgaa ttgcccgtca tattgccgga
     4441 cacgctgctt cattacatcg cctggatgga attatattca ccggcggaat aggagagaat
     4501 tcaagtttaa ttcgtcgtct ggtcatggaa catttagctg tattaggcgt agaaattgat
     4561 acagaaatga ataatcgctc taactctttt ggagagcgaa ttgtttccag tgaaaatgcg
     4621 cgtgtcattt gcgccgtaat tccgactaac gaagaaaaaa tgattgcttt ggatgccatt
     4681 catttaggca aagtgaacgc gcccgcagaa tttgcataat ttagttgaag tattgtagag
     4741 agattatttt tcatgaaggt agatattgat accagcgata agctgtacgc cgacgcatgg
     4801 cttggcttta aaggtacgga ctggaaaaac gaaattaatg tccgcgattt tattcaacat
     4861 aactatacac cttatgaagg cgatgaatct tttctcgccg aagcgacgcc tgccaccacg
     4921 gaattgtggg aaaaagtcat ggaaggcatc cgtatcgaaa atgcgactca cgcgccattg
     4981 gatttcgata ccaatattgc cactacaatt accgctcatg atgcaggata tattgaccag
     5041 ccgctggaaa aaatagtggg tctgcaaacg gatgcgccat taaagcgtgc gctgcatccg
     5101 ttcggcggca ttaatatgat tagaagttca ttccacgcgt atggtcgtga aatggatagc
     5161 gaatttgaat acatctttac cgatctgcgt aaaacccata accagggcgt atttgatgtt
     5221 tactcaccgg atatgctgcg ctgccgtaaa tctggtgtac tgaccggttt accagatggt
     5281 tatggtcgtg ggcgtatcat cggtgactac cgccgcgtgg cgctttacgg catcagttat
     5341 ctggtacgtg aacgcgaact gcagtttgcc gatctccagt cccgtctgga aaaaggcgaa
     5401 gatctcgaag ctacgatccg tctgcgtgag gagctggcgg aacatcgtcg tgcgctgttg
     5461 cagattcagg aaatggcggc gaaatacggt tttgacatct cccgtccggc gcagaatgca
     5521 caggaagcgg tgcagtggct ctacttcgct tacctggcgg cagtgaaatc gcaaaatggc
     5581 ggcgcgatgt cgctggggcg cacggcatcg ttcctcgata tctacattga gcgcgacttt
     5641 aaagcaggcg tactcaatga gcagcaggca caggaactga tcgaccactt catcatgaag
     5701 atccgtatgg tgcgcttctt gcgtacgccg gaatttgatt cgctgttctc cggcgacccg
     5761 atctgggcga cggaagtgat cggcgggatg gggctggacg gtcgaaccct ggtgaccaaa
     5821 aactccttcc gctatttgca caccctgcac actatggggc ctgcaccgga acctaacctg
     5881 actattctgt ggtcggaagc cttgccgatt gccttcaaaa aatatgccgc gcaggtgtct
     5941 atcgtcacat cctctttgca gtatgaaaac gacgatctga tgcgtactga cttcaatagc
     6001 gacgattacg cgattgcctg ttgtgtcagc ccaatggtga ttggtaagca aatgcagttc
     6061 tttggagcac gcgctaacct ggcgaaaacg ctactgtacg caatcaacgg cggaatggac
     6121 gagaagctga agattcaggt tggcccgaaa acggctccgt tgatggatga cgtgctggac
     6181 tacgacaaag tgatggatag cctcgaccac ttcatggact ggctggcggt acagtacatc
     6241 agcgcgctga atatcattca ctacatgcac gacaagtaca gctacgaagc ttcgctgatg
     6301 gcgctacatg atcgtgatgt ctatcgcacc atggcgtgcg gcatcgcggg cctgtcggtg
     6361 gcaacggact ccctgtctgc catcaaatat gctcgtgtga aaccaatccg tgatgaaaac
     6421 ggcctggcgg tggatttcga gattgacggc gaatatccgc agtatggcaa caacgacgag
     6481 cgcgtagaca gcattgcctg cgatctggtt gaacgcttta tgaagaaaat taaagctttg
     6541 ccaacctatc gcaatgctgt gccgacccaa tcgattctga ctatcacctc taacgtggtg
     6601 tacggtcaga aaaccgggaa cacgccggac ggtcgccgcg ccggaacacc gttcgcgccg
     6661 ggcgctaacc cgatgcacgg tcgtgatcgc aaaggcgccg tggcctcatt gacgtcggtg
     6721 gcgaaactgc cgttcaccta cgccaaagat gggatctcgt acaccttctc catcgtccct
     6781 gcggcgctgg gcaaagaaga tccagtacgt aaaaccaacc ttgtcggcct gctggatggg
     6841 tatttccacc acgaagcgga tgtcgaaggc ggtcaacacc tcaacgtcaa cgtaatgaat
     6901 cgggaaatgc tgctggatgc catcgagcac ccggaaaaat atcctaacct gacaatccgt
     6961 gtctctggct acgccgtgcg ctttaacgca ctgacccgtg aacagcaaca ggatgttatt
     7021 tcacgtacct ttacccaggc gctctgacgc cggaggatgt atgaaaaaga ttatcgaaac
     7081 gcaacgtgcc ccaggcgcaa tcggccctta tgttcagggc gttgatttag gcagcatggt
     7141 cttcacctcc gggcaaattc cggtttgccc acagaccggt gagatcccgg ctgatgtgca
     7201 agatcaggcg cgtttaagcc tcgaaaacgt caaagcgatc gtggttgctg ccgggctgag
     7261 tgtgggcgat atcatcaaga tgaccgtgtt catcaccgat ctgaatgatt ttgccaccat
     7321 caatgaagtc tataagcagt tcttcgatga acatcaggcg acctacccga cccggagctg
     7381 tgtgcaggtc gcgcgtttgc cgaaagatgt gaagttggaa attgaagcca tcgcagtacg
     7441 tagtgcgtaa ggcctcgtga gcgggacggt cgcaaggtcg ttccgctcca ctttactgaa
     7501 cggcaatccg agggtgtgga tatgattagt gcattcgata ttttcaaaat tgggattggt
     7561 ccctccagtt cgcatactgt ggggccaatg aatgccggaa aaagttttat tgatcggctg
     7621 gaaagtagcg gcttattaac cgcgacgagc catattgtgg tcgatctgta cgggtcgttg
     7681 tcactgacgg gcaaaggcca tgccacggat gtcgccatca tcatgggact ggcaggaaac
     7741 agtccgcagg atgttgtcat tgatgagatc cctgcattta tagagttagt gacgcgcagc
     7801 gggcggttgc cggtggcttc tggcgcgcat attgttgatt ttcctgtagc aaagaacatt
     7861 atcttccatc ccgaaatgtt gcctcgccat gagaacggaa tgcggatcac tgcctggaaa
     7921 ggacaggaag agctattaag caaaacctat tactctgtcg gcggcgggtt tattgtcgaa
     7981 gaagaacact tcggcctgtc gcacgatgtc gaaacacccg taccttacga tttccactca
     8041 gcaggtgaac tgctgaaaat gtgtgattac aacggcctgt ctatatctgg cctgatgatg
     8101 cacaacgagc tggcgctgcg cagtaaagcg gaaattgacg cagggtttgc ccgtatctgg
     8161 caagtgatgc atgacggtat tgaacgtgga atgaatactg aaggcgtgct gcctggtccg
     8221 ctcaatgtgc cgcgccgtgc cgtagcgctg cgtcgtcaac tggtttccag cgataacatc
     8281 tctaacgatc cgatgaatgt catcgactgg atcaacatgt acgcgctggc ggttagtgaa
     8341 gaaaacgcag ctggcgggcg cgtggtaacg gcaccgacta acggtgcgtg cggcattatt
     8401 ccggcagtgc tggcttatta cgataagttc cgtcgtccgg taaacgagcg gtcaattgcc
     8461 cgctattttc tggcagcggg ggcaattggc gcgctgtata aaatgaacgc ctccatctct
     8521 ggcgcggaag tcggctgtca gggggagatt ggtgtagcct gttcaatggc ggcggcaggg
     8581 ttaactgaac tactgggcgg cagtccggcg caggtatgca atgcggcgga aatcgcgatg
     8641 gagcataacc ttgggctgac ctgcgatccg gttgccggac aggtacaaat cccgtgcatt
     8701 gaacgtaatg ccattaatgc cgtgaaagca gtaaacgccg cgcggatggc aatgcgccgc
     8761 acctcggcac cgcgtgtttc actcgataaa gtgatcgaga cgatgtatga aaccggcaaa
     8821 gatatgaacg ataaataccg cgaaacatca cgcggaggac tggctattaa agtggtctgc
     8881 ggctgacatt gctgtcagct atcatccttg ggtgcacaaa atgtgcaccc ttttttattg
     8941 tgatctgccg caaatagaga ataattattt ctattaactc aatttaatag aaaattatta
     9001 cccacaattg ctatttaaaa gtcttgattg tgaagatttt ctctgatctc acgcgtatca
     9061 tttctttatt ctaataactc gcctgcaaaa acgtagaccg catatcgcct tgatgcggct
     9121 cggaaaaaaa taataagccc tacaaaatat tgagcgagaa attatggaaa ttgcatcgaa
     9181 taaaggcgtc attgcagacg cttcgacccc ggcgggtcgt gctggaatga gtgagagcga
     9241 gtggcgagaa gcgatcaaat tcgacagtac tgacaccggc tgggtgatta tgagtatcgg
     9301 gatggcgatt ggcgcgggga ttgtttttct cccggtgcag gtcggtttga tgggattgtg
     9361 ggtatttttg ctctcatcgg tgattggtta cccggcaatg tatctgtttc agcggttgtt
     9421 tattaatacg ctggcagaat caccggagtg taaagattac ccgagcgtca tcagcggtta
     9481 tttaggtaaa aactggggca tcctgttagg tgcactctat ttcgtaatgc tggtgatttg
     9541 gatgttcgtc tattccaccg ccatcaccaa cgatagtgct tcctacctgc ataccttcgg
     9601 cgtgacggaa gggttgctgt cagacagtcc cttttatggt ctggtactga tttgcattct
     9661 ggtggcgatc tcctcacgcg gcgagaaatt gttattcaaa atttcgaccg gcatggtgct
     9721 gaccaagctg ctggtggtcg cggcactggg cgtgtcgatg gtgggaatgt ggcatctgta
     9781 taacgtcggt tcgctaccgc cgctggggct gctggtgaaa aacgccatta ttacgctgcc
     9841 gtttaccctg acgtcgattc tgtttatcca gacgttaagc ccgatggtga tctcttatcg
     9901 ttcgcgggaa aaatcaattg aagtggcgcg gcataaagca ttgcgggcaa tgaatatcgc
     9961 gtttggcatt ttgtttgtca ccgtcttttt ctacgccgtg tcgttcacgc tggcgatggg
    10021 acatgacgaa gcggtaaaag cctacgagca gaatatttcc gcgctggcga ttgccgcaca
    10081 gtttattagc ggtgacggcg cagcgtgggt gaaagtggtc agcgtcattc tcaatatctt
    10141 tgctgtaatg acggcattct ttggcgtcta tttaggcttt cgcgaagcaa cgcaagggat
    10201 cgtaatgaac atcctgcgtc gcaagatacc tgccgagaag attaacgaaa atctcgttca
    10261 gcgcggcatc atgattttcg ccattttgct ggcctggagc gccatcgtac tgaacgcccc
    10321 ggtgttgagc ttcacctcta tctgtagccc gattttcggc atggtagggt gcctgatccc
    10381 gggtggctgg tttacaaagt accggcattg cacaaataca aagggatgtc tctgtacctg
    10441 attatcgtca ctggtttgtt gctttgtgtt tctccgttcc tggcattttc ttgatttacc
    10501 tgaaatttta aggtttttaa tatgtttgat tcgactttaa atccgttatg gcagcgttac
    10561 atcctcgccg ttcaggagga agtaaaaccg gcgctggggt gtactgaacc gatttcactg
    10621 gcgctggcgg cggcggttgc tgcggctgaa ctggaaggtc cggttgaacg tgtagaagcc
    10681 tgggtttcgc caaatctgat gaagaacggt ctgggtgtca ccgttcctgg tacaggaatg
    10741 gtggggctgc caattgcggc ggcgctggga gcgttaggtg gaaatgctaa cgccgggctg
    10801 gaagtgctga aagacgcaac tgcgcaggca atttccgatg ccaaggcact gctggcggcg
    10861 gggaaagtct cagttaagat ccaggaacct tgcgatgaaa tcctcttctc acgcgccaaa
    10921 gtctggaacg gtgagaagtg ggcgtgtgtc accatcgtcg gcgggcatac caacattgtg
    10981 catattgaga cgcacaatgg tgtggtgttt acccagcagg cgtgtgtgac agagggcgag
    11041 caagagtcgc cgctgacggt gctttccagg acgacgctgg ctgagatcct gaagttcgtc
    11101 aatgaagtcc cgtttgcggc gatccgcttt attctcgatt ctgcgaagtt aaattgtgcg
    11161 ttatcgcagg aaggtttgag cggtaactgg gggctgcata ttggtgcgac gctggaaaaa
    11221 cagtgcgcgc gcggtttgct ggcgaaagat ctctcttcat ccattgtgat tcgtaccagc
    11281 gcggcatccg atgcgcgtat gggcggcgct acgcttccgg caatgagtaa ctccggctcg
    11341 ggtaaccagg ggatcaccgc aacaatgccc gtggtggtgg tagcagaaca cttcggagcg
    11401 gatgatgaac ggctggcgcg tgcgctgatg ctttcgcatt tgagcgcgat ttacatccat
    11461 aaccagttac cgcgtttgtc tgcgctgtgt gccgcaacga ccgcagcaat gggggccgct
    11521 gccgggatgg catggctggt ggatgggcgt tatgaaacca tctcgatggc gatcagcagt
    11581 atgatcggcg atgtcagcgg catgatttgc gatggtgcat cgaacagctg cgcgatgaag
    11641 gtttcgacca gtgcttcggc tgcgtggaaa gcggtgttaa tggcgctgga tgataccgcc
    11701 gtaaccggta atgaagggat tgtggcgcat gatgttgagc agtcgattgc caacctgtgt
    11761 gcgttagcaa gccattcgat gcagcaaacg gatcggcaaa ttatcgagat tatggcgagc
    11821 aaggccagat aaacgccagg ttttgggtat tgtgccggat aaggcgttta cgccgcatcc
    11881 ggcaacttgt ctttacgcgg tagcttcatc ttcggctaaa cgcgtttcag catccgcgac
    11941 gatggcttcc agctcgctca acatctcttc atagccaaaa tttttggcgg tacgagcagg
    12001 ttctttcgcc accacgggct tcactggctg gcgctttttg gccgattttt tactcatcat
    12061 catctccctg taaaaacaac tagacaggca aatctatcag caaagcgcgc agtggggaat
    12121 cggcaaccag cgtaatgtta gcctcgtcac gaataaacgc cccatcaccg caggttagtg
    12181 ccgctttctc tttatgatgc gtaagcgcat gaaatttccc gtgaatcgat tgcaaatacg
    12241 cgcgtggccc atgcaactgg aaattcgcac tttcgctttt gtcgagcacg atatggtgca
    12301 gccacacttg ctggcgcaat tgcaggcttc ccatcccacc ttctggcgag gcgattaatt
    12361 gctgcttgct catattaagc gccagctttt gaatcagcgg attctctcgc tgcgggcagg
    12421 catccagcca aagctgcatt cgcgttaacg gtttgtcttt gctgagattg tgttcgctat
    12481 agctgacacc cggctgggta gagagcagca acacctcacc ggcgctggcc tgaacatgat
    12541 tgccttcgct gtcgcgatac tcagcttctc catccagaat cacatttaaa atatcgactt
    12601 tgggataggt tcgcggctga aaggcggcac ctggggccag cacttcctgg ttaagcacgc
    12661 gcagggaggc atagcctaac aatttcgggt cgaagtagtg tccaaaggaa aaagtatacc
    12721 gggcctgcaa ccatccgtag tctgcttgtc cacactgcct ggcagttcgg gtagtaatca
    12781 taattcttga cctctcttta cttcctttta tggtaagggg ctggacgcta cattgttagc
    12841 cagatattct gcccggtatg ttcaaatttc ctgaatgaga acgaaatggc caaagaaagg
    12901 gcattaacgc tggaagcact acgggttatg gatgcgatcg atcgccgggg cagttttgcg
    12961 gcggcggcgg atgagctggg acgcgtgcct tccgcactta gctacaccat gcaaaaactg
    13021 gaagaagagc tggatgtggt gctgtttgac cgctcgggcc atcgcaccaa attcaccaat
    13081 gtcgggagga tgttgctgga acgagggcgc gttttgctgg aagccgcaga taaactgact
    13141 accgatgcgg aagccctcgc gcgcggttgg gaaacgcatc tcaccattgt gaccgaggcg
    13201 ctggtaccga cacctgcctt tttcccgtta atcgacaaac tggcggcaaa agccaatacc
    13261 caactggcaa ttatcaccga agtgctggcg ggggcg
//
LOCUS       DAZFRE010000118        13231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFRE010000118 DAZFRE010000000
VERSION     DAZFRE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.118"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            73..1761
                     /locus_tag="ACHKUI_004220"
     CDS             73..1761
                     /locus_tag="ACHKUI_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO6600301.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGIPV
                     AERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            1758..2672
                     /locus_tag="ACHKUI_004221"
     CDS             1758..2672
                     /locus_tag="ACHKUI_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6600302.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2704..2952
                     /locus_tag="ACHKUI_004222"
     CDS             2704..2952
                     /locus_tag="ACHKUI_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6600303.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2952..4124
                     /locus_tag="ACHKUI_004223"
     CDS             2952..4124
                     /locus_tag="ACHKUI_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6600304.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="ACHKUI_004224"
     CDS             complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="ACHKUI_004224"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6600305.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="ACHKUI_004225"
     CDS             complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="ACHKUI_004225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6600306.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6336..6896)
                     /locus_tag="ACHKUI_004226"
     CDS             complement(6336..6896)
                     /locus_tag="ACHKUI_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6600307.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6908..7732)
                     /locus_tag="ACHKUI_004227"
     CDS             complement(6908..7732)
                     /locus_tag="ACHKUI_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600308.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(7732..9081)
                     /locus_tag="ACHKUI_004228"
     CDS             complement(7732..9081)
                     /locus_tag="ACHKUI_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO6600309.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9127..9900)
                     /locus_tag="ACHKUI_004229"
     CDS             complement(9127..9900)
                     /locus_tag="ACHKUI_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600310.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSSA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9932..10645)
                     /gene="yghS"
                     /locus_tag="ACHKUI_004230"
     CDS             complement(9932..10645)
                     /gene="yghS"
                     /locus_tag="ACHKUI_004230"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6600311.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
     gene            10819..11511
                     /gene="yghT"
                     /locus_tag="ACHKUI_004231"
     CDS             10819..11511
                     /gene="yghT"
                     /locus_tag="ACHKUI_004231"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6600312.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRSVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEK
                     AITDFMTARGYH"
     gene            complement(11560..13059)
                     /gene="pitB"
                     /locus_tag="ACHKUI_004232"
     CDS             complement(11560..13059)
                     /gene="pitB"
                     /locus_tag="ACHKUI_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO6600313.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            12996..>13231
                     /locus_tag="ACHKUI_004233"
     CDS             12996..>13231
                     /locus_tag="ACHKUI_004233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600314.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKSVLFLVYQTISIHSKPLEIIKTQVF"
BASE COUNT         3357 a         3424 c         3257 g         3193 t
ORIGIN      
        1 gtcattttat ttttgatatc caattttttc gtggtgtata tgtctaataa aatctttacg
       61 cattccctac ctatgcgcta tgccgatttt ccaacgctgg ttgatgcttt ggactatgcc
      121 gctctgagta gcgctggaat gaatttttat gacagacgtt gccaacttga agatcaactg
      181 gaatatcaga cgttaaaagc gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac
      241 ctgaaaaaag gcgatcgcgt ggcactgatt gccgaaacga gtagcgggtt tgtagaggct
      301 ttttttgcct gccagtatgc cggcttagtc gccgtcccgt tggcgattcc aatgggcgtc
      361 ggtcagcggg attcctggag cgccaaactg caggggttac tggcaagttg ccagcccgca
      421 gccattatca ctggtgatga gtggttgcca ctggtcaatg ccgcgacgca taacaatccc
      481 gaattacatg ttttaagcca cgcctggttt aaggcattac cggaagccga tgttgcgctt
      541 caacgtccag ttccaaacga tatcgcctac ctccagtaca cctccggcag cacccgtttt
      601 ccccgtggcg ttatcattac tcaccgcgaa gtgatggcta atctacgtgc tataagccac
      661 gatgggatta aattacgccc tggcgaccgc tgcgtctcct ggctgccttt ctaccatgat
      721 atgggactgg tcggctttct cctgaccccc gtcgccacgc agctttcagt agattatttg
      781 cgcactcagg attttgccat gcgtcctctg caatggctta aattgatcag taaaaatcgc
      841 ggcaccgttt ccgttgcgcc gccgtttggc tatgaattgt gccagcgccg cgtgaatgaa
      901 aaagatctcg ctgaactgga tctttcctgc tggcgcgtcg ctggtattgg tgcagaaccc
      961 atctccgcag aacaactcca tcaattcgct gaatgtttcc gtcaggtcaa ctttgacgat
     1021 aaaactttca tgccgtgcta cggactggca gaaaatgcgc tggctgtcag cttctctgat
     1081 gaagcttccg gggttgtggt taacgaagtg gatcgcgaca tcctcgaata ccagggtaaa
     1141 gccgtcgcgc cgggtgctga aacacgcgcc gtatcgactt tcgtcaactg cggtaaagcg
     1201 ttgccggaac atggcattga aatccgcaat gaagcaggta tacctgtcgc ggaacgtgtg
     1261 gtaggccata tttgcatctc cggccctagc ctgatgagcg gttactttgg cgaccagatt
     1321 tcacaagacg agattgccgc gacgggctgg ttagacaccg gcgacctcgg ttatctgctg
     1381 gacggttatc tgtatgtcac cggacgcatt aaagatctga ttattattcg tggccgtaat
     1441 atctggccgc aggatattga atatattgcg gaacaggaac cggaaattca ttctggcgat
     1501 gcgattgctt ttgttaccgc ccaggaaaaa atcattttgc agatccagtg tcggatcagc
     1561 gatgaagaac gtcgcgggca gcttatccac gcgctggcag cacggatcca aagcgaattt
     1621 ggcgttaccg cggatatcga tctgttgccg ccccacagta ttccccgaac atcctccggc
     1681 aagcctgccc gtgcggaagc gaaaaaacgt tatcagaagg cttatgctgc cagtcttcat
     1741 gtgcaggagt ccctggcatg aatcaaactg tcgcggtgac gggcgctacc gggtttatcg
     1801 gtaaatatat tattgataac ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta
     1861 ctgcccgcgc tcacgtcaat gataatctca cttgggtgcg cggttcgctg gaagatacac
     1921 attcgcttag cgaactggtt accggagcca gcgcggtggt ccattgcgcc gggcaagtgc
     1981 gcgggcacaa agaagagatt tttacccgct gcaacgttga cggcagcctg cgcctgatgc
     2041 aagcggcaaa agagagcggc ttttgccaac gatttctgtt tatctcttcg ctggcggcgc
     2101 gccatcccga actctcctgg tacgcaaatt ccaaacacgt cgccgaacaa cagctgactg
     2161 caatggctga cgcaattacg ttgggcgttt ttcgcccgac agccgtgtat ggtcccggcg
     2221 ataaagagtt aaaaccgctg tttgactgga tgctgcgcgg tctgctgcca cgacttggca
     2281 cgccagagac acagctctct ttcctgcacg tcaccgattt tgcgcaagca gtgggtcagt
     2341 ggttaagcgc cgaaactgta cagacgcaaa cctatgaatt atgcgatggc gtcgccggag
     2401 gctatgactg gcaacgtgtc caacagcttg ttgccgatgt ccgctgtggt tccgtgcgga
     2461 tggtcggtat tcctctgccg ctactcacct gccttgcgga tatcagtacc gcgttgagcc
     2521 gtctggcggg taaagaaccg atgctgactc gttcgaaaat ccgtgaatta acccatgccg
     2581 actggtcggc aagtaataac cgtatttctg aagatattaa ttggtttccc gggattagcc
     2641 tggaacacgc attacgcaac gggctatttt gattgtcttg tttaaaggtg tgttaacgtt
     2701 tctatggtaa atcgtgaaat agtaatggat tacatccttt cctgtttaca ggatttggtc
     2761 gaaaacggtg tggaaattaa gcccgacagc gatctcgtca acgatcttgg cctggagtcg
     2821 atcaaagtga tggatctctt aatgatgctg gaagacagat tcgatatttc cattcctatt
     2881 aatattctgc tggatgtcaa aacccctgcg caattaatgg aaactctact tccatggctg
     2941 gagaataaat aatggggcta tacgataaat atgcgcgcct tgctggcgag cgactgcaat
     3001 ttagtgacaa cgggttaaca ccgtttggca cctgtattga tgaagtgtat tccgccaccg
     3061 aaggtcggat cggcaataaa aaagtgattc tggccgggac caataattat ctcggtttaa
     3121 cctttaatca tgatgccatc gccgaaggtc aggcagcgtt agcggcccaa ggcaccggca
     3181 ccaccggttc tcgtatggca aacggcagtt atgcgccgca cctggcgtta gagaaagaga
     3241 ttgccgagtt tttcaatcgc cctaccgcta tcgttttttc aaccggttat accgctaatt
     3301 taggcgtcat cagcgcactt gccgaccata acgccgtggt gttactggat gccgacagcc
     3361 acgccagtat ttatgacgcc tgttctctcg gtggcgcaga aattattcgt ttccgccata
     3421 acgatgctaa agatctggaa cggcgcatgg ttcgcctcgg tgaacgggcg aaagaggcga
     3481 ttatcatcgt tgagggcatc tacagcatgc tcggtgacgt tgcgccgtta gcggaaattg
     3541 ttgatatcaa acgtcgtctc ggtggttatt tgattgtcga tgaagcgcat tcgtttggcg
     3601 tgttaggcgc aacggggcgc ggactggctg aagccgttgg cgttgaagat gatgtggata
     3661 ttatcgttgg tactttcagt aaaagtctgg cctcgattgg cggttttgct gtcggttcgg
     3721 aagcgatgga agtgctgcgc tacggcagcc gcccgtatat ttttaccgcc tctccttctc
     3781 cgtcctgcat tgcgaccgtg cgctcgtcgt taagaaccat tgccacacaa ccagagctgc
     3841 ggcaaaaact gatggataac gccaaccatc tgtatgacgg tctgcaaaaa ctgggttatg
     3901 agttaagttc acatattagc ccggtagtgc cagtaataat cggttcgaaa gaggaaggtt
     3961 tacgcatctg gcgcgagctt atttcgctgg gcgtttatgt gaatcttatc ctgccacctg
     4021 ccgcgcctgc aggcatcact ctgctgcgct gtagcgtaaa cgctgcccac agtcgtgagc
     4081 aaatcgatgc catcattcag gcatttgcta ctctgaagca gtaatgtgag cccgctgtgg
     4141 tgttgcgcca cggcgggttt aatgttgtct gtcataccaa tacaccaggc tcaacgccac
     4201 caacacaaac gctaccggta aaccgagcgc ggtaacaggt gcgctcaacg caaataacaa
     4261 tcccagattg accatgattt gatagctgat ccaggtgagt aagccaacga tgacgcctac
     4321 agcgagacgg ctacccatcc ctggcgagcg cggggcgcta aaggtgaacg gcacagccag
     4381 caaaatcatc gccagggtca ggatcggttg ccccagtttt tcccacagtg caaggcggta
     4441 ttcgctgctg ggttgtccgg tttcctgcaa ataatggatg taatgattaa gctgacggac
     4501 agaaaaagta ttccccggca tcgataactc ttcaagatcc atactggtga aggccgattg
     4561 ccaggcaaga ttatctgatg tttccagcgt ttctttgcca ttcaaccatt ttttatgatt
     4621 cacaccatgc aggatccacg ttttgtcgtc ttcaatggag gcactgcgtg caaaaatgta
     4681 ggattcgagc gagagatcgt cgcgataatg aaatatctcc acgcccacag gctggccctg
     4741 ctcgttcagc gatttcaccg tcacaaattc attaccgcgc ctggcccaaa gcatattgcc
     4801 ggtaatgtca tcgtcctccc ccaacgcggt ggcagtcgat ttgatttgca gcgcctgttg
     4861 ctgcaatggc gacgccaccc actcatctat cgcgcctaac gaaacagtcc acaatatccc
     4921 ggcaaccagc gccaccagtg caatacggaa gatagaaaac ccagtgctgc gaatggcggt
     4981 aagctcactg tttttcgata actgccccag gccaacaatc cctcccaata atgcgataaa
     5041 cggcgaaagt tcgaccagtg tacgcggtaa ggtcatcagc accaccagca ccgcctgagt
     5101 ccagcgataa ccgcccgggc tgacatcatc cagttcgtta atcaggttga aggtggtaaa
     5161 aagcggcagc aatagccctg cggcggcggc aaaaccgaga aagagatgac ggattaaata
     5221 gcgactgaaa acattcatcg ggaaaatttc tgcagcaaag agaagtcgcg tgcgaccagc
     5281 atcagcaggc ccattagcat gagtccgggt actaaccaca aaccaggaat gaggggaatc
     5341 gcaccgttag ccaccagcgt acggcagacg tcgccgccat aaaaaatggc aacaaacagc
     5401 aatgtcagtg gcaataacgt tgaaaatcgc ccttgccgcg gttttacccg acttaatgaa
     5461 atggccagca gcgccatcaa caatgctgtc aggccacggc tttgtcgcca ttgcagttcg
     5521 gcatgatcgg caggaaaggc tgagcgcgcc agctccgtga ccgattttgc tttgcgtttg
     5581 acgttagggc tttgatccag cggattcaga tgtaattgca gattacggta gatctgttcg
     5641 ttgtcgtcac gcccctgatg atcgagaaga taggcggtcc cgttatgcaa cataacggtc
     5701 ggtttctcag gtgatgggtc aaccacatca accgaacggg cgcggaaaat gcgggttcga
     5761 ttggcagtag aagtataaat cagcgcgtca gtcaggcgat tattatcctg atcaaccgtc
     5821 tgcgaaagga tcattcgtcc gttatcgtta gtgttaaatt tctttgcccg caactggcga
     5881 acatccagct ccgactgcga ctgttgctcc agttgataaa tctgcgcata agcccacggc
     5941 cgaccataca tcgacagaag ggtgacaaaa atacttagcg gtatcgccag ataaagaacg
     6001 gctttgtaca agcgtcccgg attgcccccc gccgcagaga tagcggtaat ttccgagtcg
     6061 gtatacatct gccctagcgt cacgccaact gacacataca gaccaacagg taacaacatc
     6121 tccagtgcaa tcagcacttt gtagaaaacg atatccagca caacatcaag agctaaggtc
     6181 ccatttgccg cttcggtcag ataacgctgt gcggagtagc tggcgaaaat aaagatcaga
     6241 aaaccgacga taatcagcac cagacggcgc gtaccacgca tgatgtagtg ttcaaccagc
     6301 ttcatctcac taacaccctc aatcctttga attttttatt cattgatcag tttctgcgcc
     6361 agcgcaatga catgatctac ctcttccggc gtcaggctgc cgtcctttgc ccactgaatt
     6421 tgcccgtctt tattcagcac cagaatggtt gaactctgct ccggcaaccg ccaggcaacg
     6481 cgtcccagtc cattgccgtc aataacgaat tgcgcccacg gatagcggcg tttatttttt
     6541 tcgattttgc caacgacaaa atagccagta ccaaagatgg catcatcggt gttcacaatg
     6601 gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt caaccgcttt aattaacagt
     6661 gagttctttt tcttcgccga cttacgcccg gcgatatact ggataatgcg gacttttccc
     6721 gccagctgtg tgctgttcca cttttggtag tgaatttcgt cgtcgctatt cagaagcagc
     6781 tcgccgcctt cctggatata aacgggggta accgttttgc cagtgacaaa attgtgtgcc
     6841 tgcacaccct gggctaagag catgataatc agcgcaataa tcaaacgaga tgacatcatt
     6901 gctccggtta tagcggaaaa cgcgaaatca gcagctctaa gcgctggcga atatcgctat
     6961 ccagcgccac cggtgcatca tacgactggc ggttttccat atcatccgcc accatgtcgt
     7021 cgtgtttatc ccccatcagg ccattaatgg cggcgaggaa gcgggaaatc tgtaacgcct
     7081 ctttattttc aggatcaagc gatcttaagg tgtcgtaata agaaacctgc tggctgatga
     7141 tttttcgcac atcatgaaat tccctgccgg tgagtcgcgg tttggcaatt atctctttca
     7201 gggtatgcat ttgcttttgc cgataggcaa taaaaccgct tacgctttct aactggctat
     7261 ggcgtaatga atagttcacc aacgaccaga ccggagagct taaataaacc cgtaacaagt
     7321 tgccgtaata cgacacaata tttttcttac cgttacgaaa accgtcctga aaacgcccca
     7381 aaaaaacggt cgttttgccg aataaaaatc cggcctgatg cttctttaaa tacagccgtt
     7441 gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt aaattgtttg cgctcatcag
     7501 cagtcagctc atcgcctttg gcctgtttgc atattaaacg cagtaactcc tggcgattga
     7561 cgccattgag ccagaattgc aggcataacg cgtaattatc atggatgatt tcggtggtac
     7621 aacttagtgc gatagtcggc ggcaaactga cctgtgcatc aacgacatca tcttcttcaa
     7681 ctgcaacaaa taaacgctcg ctcgcctcaa gcgggaaata ttttctcatc atcaggattt
     7741 ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa aatgccttta aggtcggaat
     7801 taacgagagc accacatcca gacattgccc accgaaataa gcaaacgctg ctcccagcac
     7861 gccaaacaga tgacagaaca tgaacagcaa tacgatgtag ccgattgagg cgatggtttg
     7921 cgccacgaga aaagcgcgct gttttcccgc catcaatagc aatgactcct gtgggaagcc
     7981 cagcatcgaa atcacaattg cgcccagcat cacctgaatc aggtcatacg cttcgagata
     8041 cttaacgcca aacaccagcg aaataagcgg cttgccgacg atcaacaccg cgagcgccac
     8101 cagaatacca attccgcccg ccagtaaacc tgacttcacg cctaacaacc acggtctggt
     8161 ggtgcgcgga tctaaacgca tcacctctgg gtaaaaactt ttgcccagta aacccgctgg
     8221 cgttccggcg gcgtcgaaga atgtcatggc gattttaaat aacccggcgg cagcgggtcc
     8281 taacacgatc cccaccagca ctgtgctgca cgagttacgc gccgaccaga tggagtgggc
     8341 aatgttggtt gaccagacaa aactccacgc gcctttaatg tgtcgggcag actcaaacag
     8401 attcaatttg aaggcgttat ggatatttct gcggcgtaat tcgcgcgcgg caaaccacca
     8461 gtacatggtg ccgccaacca gattcgacac gtaccaggca ataacaaaac ccgcaaaacc
     8521 aaagtcaaaa taccaggcta cgacgctccc cgctgcgcgc agaaaaggtt tcgtcgcttg
     8581 ctgtacagca attagatcga agcgatctac cgcacgcaga atgccggtcg gcgtggagga
     8641 agccattgaa ggaatgagcg tgcaatacag cgctgccagc caaaagcttt ggtcatccag
     8701 acctaatgaa tgggaaagaa aagggagtaa ggcaatgccg ccgacaatcg ccaccgcgcc
     8761 gctgacgata tccagcgaga aggaaaatga gacgacattg cggaattgct gcggattatt
     8821 gttggttaat gctggtgttc cgtactgaac caccagctgc catatctgaa acttaataaa
     8881 atcgctgatc gacttggcgt acgattgtac aatcaccagt acgccaaaca tggcgggcgt
     8941 catcccttta ccggcacacg agagcgccaa cagacccagt aaagcgctca cgacattact
     9001 ggagcctaac caggcgctat tgcgaataat ggtgcgaaac gcgccatctg caaaccaatg
     9061 tttgatgtta aaacccgcca attcagcctg accttatcgt tgatagtaaa aagtatcccg
     9121 ccagccttaa gttaaacttc ggcggtcaga aacgatggca accagagaaa ccgccttctg
     9181 tgcctgttcc agcacttcgc tgtagggcgc tctggaatca atctcaagaa tttttgtgcc
     9241 gttatagcca atcgtcgaca tgacgccgat tttgtcctgc agctcggcat agtcatggtc
     9301 aggcttgcgg gagatggcag tatcaatatc aatccccaaa cgaatgatta attcaggacg
     9361 gtattgcgcc attttttggt ataaacgccg ttcgcgctgc gccagaaaca tgctgatttt
     9421 cccggtcgca cgttcgacgc cgatccccgg tccgtcataa taaaagcccg aaatttcagc
     9481 ctgcgggaag cgatcgctga ccaccagaac gccactttgc gccagtcgct gaaccttgcg
     9541 tagattcgcc attcttcgca gcgagaagca gtacataatc accgccgccc atagcgctga
     9601 agatttggtt ttcatgcttt gggttttcat gctttgggtt ttcgatgatt tggccgccag
     9661 tcgcctttcg agccagacgc caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc
     9721 cgagagcagc cccagatagc gccgctcggt ttgccagtgt tgttgcagcg atttcacgag
     9781 gtcggtggtc agtgtggatt taccggtgcc atcacacccg accaccgcaa tcagccccgg
     9841 aatgtagttg ggctgcggcg cggttgtact attaacagtt tgtgtttgta gtgcgtccat
     9901 tcgttaaatc atcccctatc cattttggtg atcaggaaag cgcagcttga attgcgcgta
     9961 gtgacgcttg cagaatttca tcggctggtt gccgaccatc cagttcaagg atcctggcgc
    10021 cattaaatgt cagttgcggc gtaacggcga ttttttcctg taacgccgcc agttggtggt
    10081 caggtttacg cgcaaacgcg gtttgttcat caatgccaag acgaatcaac aatacgggca
    10141 aataagatgc catccattgg tacagcttca gctcgcgctg ccttaacatt tttatccaac
    10201 cgttaccgcc cgtggttttt gccaattgcg ggccatcaaa gcgaaaaccc ggcacttcaa
    10261 cctgcgggta gcggtcggtg atgagcagat agccttgctg gcttttacac aacattttgc
    10321 gaaacttata cgcccgccag caggaaagca gatagataac cagcgcagtc atattgccag
    10381 gcggtgtgga gggcttttcg tgcacatgtg ccgctttact tcgcagataa cgcccaaaag
    10441 gtgcgccaat aacagggagc tgtgaaatcc attcgccaat tcgcccggac gattgcccga
    10501 gataaatgtg ttctgttggc attgttgctg ccagttcatt taccaggctt gccgtgaggg
    10561 tcgatttacc tgaaccatca catcctacaa tggcaataac acgcactggc gtggaattaa
    10621 ttattgacat attacgttga ttcacgaaaa acccggcagc aaataatgta ttgaatatta
    10681 cgtggtccgt gctcagaata tccgttcaac cttattgtga caatggttaa ttgtgacatt
    10741 gacctgaatt acgctttcat aaaaacatat taaccaaata aatattttta atggatattt
    10801 aaattaaagg atatattcat gcagtcaata acacctccat taattgccgt tattggtagc
    10861 gatggttcag gcaagtcaac ggtgtgtgaa catctcatta ccgttgtcga aaaatatggt
    10921 gctgccgaaa gagttcattt aggaaaacag gccggaaatg tcggtcgttc agtgacaaaa
    10981 ttaccgttga tgggaaaatc cttacataaa acaattgaac gaaatcaggt gaaaacagca
    11041 aaaaaattgc caggaccagt tccggcgctg gtaattacag cgtttgtcgc ccgtcgctta
    11101 ctgcgctttc gtcatatgct tgcctgtcgt cgtcgcgggt taattgttct aaccgaccgt
    11161 tatcctcagg accaaattcc tggcgcttac gatggtacag tgttcccacc taacattgaa
    11221 ggtggtcgtt ttgtctcatg gctggcaagc caggaacgta aagcgtttca ctggatggcg
    11281 agccataaac ctgatctggt catcaaactc aatgttgacc ttgaagttgc ctgcgcgcgt
    11341 aaacccgacc ataaacggga atcgctggcg aggaagattg ccataacgcc acagttaacc
    11401 tttggcggtg cacaactggt tgatatcgat gctaatcagc cactggaaaa ggtgctgatt
    11461 gatgcagaaa aagcgattac ggattttatg accgcgcgtg gttatcacta gtcaaaaatg
    11521 gaaatgcccg atcgccagga ccgggcattt tcaggaaggt taaatcaact gcaatgctat
    11581 ccagtacaac ccaccagaaa gaaaaatcgc cgccggtaaa gtaaacaccc acgccatcag
    11641 gatgctggtt accgttttac gctgtaaccc accgccgtcc accaccatcg ttcctgctac
    11701 ggcagatgag agtacgtgtg ttgtggagac gggcatccca atataactgg caaggccgat
    11761 agacactgcc gccgtcattt gtgccgccat gccttgcgca tacgtcatgc cgcgcttacc
    11821 aatcttctca ccgatggtca tcgctacacg acgccagcca atcatggtgc caatgccgag
    11881 cgccagtgct actgccatga tgatccatac cggtgcgtac tcaatggtgc ttaacatatc
    11941 gctgcgcagt tttttcagca ggttctggtc ttctttactg acgcctggca gtttcgccag
    12001 cttcgcggaa gtatcagaga tgcacagcat aatgcggcgc agctggctgc gctgactcac
    12061 gcttaacggc tcgtagcttt ccatattgcc tggcagcatc gttttaacgc gcgcaatcgc
    12121 atcaaaggta tttgccggat gacagtgaaa ctccgtcact tgcgcgccat cagtcgatgc
    12181 cgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc agttcaggat gctgttgcag
    12241 gtagtgttcg aagttggtga cggcatcgcg ggtacgggta atttcatagc cggacgcgtt
    12301 catattgacg acgaagccag caggggcaat ccccaccagt accagcatta ccaggccgat
    12361 ccctttctgc ccgtcgttag cgccgtgcga aaacgccacg cccgcagcgg aaacaatcag
    12421 cgcaatacgc gtccagaatg gcggcttacg tttgcctttt ttctttttgc gatcttccgg
    12481 aatgcggtga atacggtcac gctttttcgt cccgctccag aaacggcgca gcaggaaaat
    12541 cagaccaccc gcaatgacca ggccgacgat aggggaaaca atcagcgagg agaaaatttt
    12601 ggtcacttca cgcaggttta acgcatccat caccgatgag ccggttaaca gcgcgttggt
    12661 taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa ctggaggccg gtaaaccaaa
    12721 gaaccacgtt cccaggttcc agataatcgc cgccagcagc atggaaaaga ccatcgccag
    12781 gccgtgagtt gaccccatat tcagcaacaa atcggttggt aacatatgga caatggcata
    12841 ggcaacgcta agtccgccca ataacacgcc aaaaaaatta aaaaatgccg ccatcaccac
    12901 agcaagttgt ggttgcatgg cacgagtata aataacggtt gccaccgcat tcgccgtgtc
    12961 atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc agagcaagta ataacaaaag
    13021 ccctgtgtat atatcaaggc caacaaataa atttagcata tattagatta cgccattttg
    13081 aatttacgaa cggacgcatt atcagtgact ttaacggcat gggcaaagtg aaatatcatt
    13141 ttttaacgat aaaaagcgtg ttatttcttg tgtatcagac aataagcatt cattcaaaac
    13201 ctttagaaat aatcaaaaca caggtattct g
//
LOCUS       DAZFRE010000119        13208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFRE010000119 DAZFRE010000000
VERSION     DAZFRE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.119"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1796)
                     /locus_tag="ACHKUI_004234"
     CDS             complement(<1..1796)
                     /locus_tag="ACHKUI_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6600315.1"
                     /translation="MSKVFICAAIPGEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPLDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAE"
     gene            complement(1890..2081)
                     /locus_tag="ACHKUI_004235"
     CDS             complement(1890..2081)
                     /locus_tag="ACHKUI_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6600316.1"
                     /translation="MNPAFALVLTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYPVDKVIHQDNIETPAGL"
     gene            complement(2078..2266)
                     /gene="dicB"
                     /locus_tag="ACHKUI_004236"
                     /gene_synonym="ftsT"
     CDS             complement(2078..2266)
                     /gene="dicB"
                     /locus_tag="ACHKUI_004236"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6600317.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEEAINKRKHERELLNKV
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(2834..3052)
                     /gene="ydfC"
                     /locus_tag="ACHKUI_004237"
     CDS             complement(2834..3052)
                     /gene="ydfC"
                     /locus_tag="ACHKUI_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6600318.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(3082..3210)
                     /gene="ydfB"
                     /locus_tag="ACHKUI_004238"
     CDS             complement(3082..3210)
                     /gene="ydfB"
                     /locus_tag="ACHKUI_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6600319.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(3212..3367)
                     /locus_tag="ACHKUI_004239"
     CDS             complement(3212..3367)
                     /locus_tag="ACHKUI_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6600320.1"
                     /translation="MDTIDLGNSEPLVCGVFPNQDGTFTVMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3660..3998)
                     /locus_tag="ACHKUI_004240"
     CDS             complement(3660..3998)
                     /locus_tag="ACHKUI_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6600321.1"
                     /translation="MKNSSITSCVQLVGEIPVNTFAVVLESDSMSTSGGGVSIPNGST
                     VFIDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            4389..4632
                     /locus_tag="ACHKUI_004241"
                     /pseudo
     CDS             4389..4632
                     /locus_tag="ACHKUI_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
     gene            4616..5041
                     /locus_tag="ACHKUI_004242"
     CDS             4616..5041
                     /locus_tag="ACHKUI_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6600322.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            5113..6183
                     /locus_tag="ACHKUI_004243"
     CDS             5113..6183
                     /locus_tag="ACHKUI_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO6600323.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTPRPEQSHQL
                     QQPEADFAIQREADRVVPENTGLSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            6224..6646
                     /locus_tag="ACHKUI_004244"
     CDS             6224..6646
                     /locus_tag="ACHKUI_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6600324.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEAMQRVLAFYQGNFQEAVL"
     gene            6704..7060
                     /locus_tag="ACHKUI_004245"
     CDS             6704..7060
                     /locus_tag="ACHKUI_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600325.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            7154..7336
                     /locus_tag="ACHKUI_004246"
     CDS             7154..7336
                     /locus_tag="ACHKUI_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600326.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            7329..7505
                     /locus_tag="ACHKUI_004247"
     CDS             7329..7505
                     /locus_tag="ACHKUI_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600327.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            7502..7868
                     /locus_tag="ACHKUI_004248"
                     /pseudo
     CDS             7502..7868
                     /locus_tag="ACHKUI_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8428..8583
                     /gene="hokD"
                     /locus_tag="ACHKUI_004249"
     CDS             8428..8583
                     /gene="hokD"
                     /locus_tag="ACHKUI_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6600328.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(8625..8804)
                     /locus_tag="ACHKUI_004250"
     CDS             complement(8625..8804)
                     /locus_tag="ACHKUI_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600329.1"
                     /translation="MPDGKVGIRIVKFGFVRHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            8751..9023
                     /locus_tag="ACHKUI_004251"
     CDS             8751..9023
                     /locus_tag="ACHKUI_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600330.1"
                     /translation="MAHKTKFHNSDTDLAIRHERRSHAFKTDWYQHPPCTEEQAEWLI
                     HSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            9025..10083
                     /locus_tag="ACHKUI_004252"
     CDS             9025..10083
                     /locus_tag="ACHKUI_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6600331.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMCG
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLCDQTSESLEQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPESVLCRSLGLPVEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPLHVHQQQPPLQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            10084..10446
                     /locus_tag="ACHKUI_004253"
     CDS             10084..10446
                     /locus_tag="ACHKUI_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6600332.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMH"
     gene            10461..11282
                     /locus_tag="ACHKUI_004254"
     CDS             10461..11282
                     /locus_tag="ACHKUI_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6600333.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYSSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHCEESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            11426..11501
                     /locus_tag="ACHKUI_004255"
     tRNA            11426..11501
                     /locus_tag="ACHKUI_004255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11459..11461,aa:Ile,seq:cat)
     gene            11602..11675
                     /locus_tag="ACHKUI_004256"
     tRNA            11602..11675
                     /locus_tag="ACHKUI_004256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11636..11638,aa:Arg,seq:tct)
     gene            12178..12309
                     /locus_tag="ACHKUI_004257"
     CDS             12178..12309
                     /locus_tag="ACHKUI_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6600334.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(12403..12519)
                     /locus_tag="ACHKUI_004258"
                     /pseudo
     CDS             complement(12403..12519)
                     /locus_tag="ACHKUI_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12590..12925)
                     /locus_tag="ACHKUI_004259"
     CDS             complement(12590..12925)
                     /locus_tag="ACHKUI_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6600335.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVAPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT         3426 a         2882 c         3381 g         3519 t
ORIGIN      
        1 tgttcagcgc cctccatttc ctccggatca tcttcctgaa cttcaacctg atactcttca
       61 tcgaatgttt cctggtatgt tgcgtcgccc atcaccgcgc cacaatcagg gcagttgccg
      121 ccgccggtct gaccgcaggc ggtgcagact ttttccactt cctgttgcgc cactggttca
      181 ggctgtttcg tttctggctc gttttgtaac gcatttgggc tgttttgttc cgctttttgg
      241 tagttccgtt ccgattcatg ctggttctgg tttacagaat cgcgggtctg gatcccctta
      301 acccatttcg gatcattcgg gtcgctaatc cctgcaacaa attctccgcg agaggcagca
      361 agcaacttat cggcgtcagg ctggctgata ttggctgcct gcataatttt gtttacttcg
      421 tcagcggtaa cttttaccgg ctctggttgt tctgaatctt cagcggtatc tacattttgc
      481 ggtaagcccg tgtatgtgcc attttttcgg gcaaaatatt cttcttttgt gatttcagtg
      541 gcgccagcag ccagtgcctt atccagacca gaaagtttgt ttgcgcgacc gtatttttct
      601 ccgtccttat ctgcgaagag gaaatagaac ggcccctcac gctctacaga tggttcagct
      661 tccggcgcgg tttcattttt tgggatatca gatacctcag tttccactgc atcagtttgt
      721 gtttctgatg actggagaac atcaacagtg cccaggtctg tttcttcatt ctcaaacacg
      781 ccctttgtcg tcaggtattc gcagatatat ttgttcagtg ctatgggatc tttgtgaatg
      841 tcgagcggac gctcacggac aaggccaaaa atagtttggc ggtcgtagcg aagggcatca
      901 ggctgtttgc gcattgatgc cgagatacgc ttccagtctt cgcggtcgtt gtcgataact
      961 tcattttttg cccagcgatg gatgctgcca tcaatgtttc cggcatccac atcaccaggc
     1021 cagagagcgt aggccagttc gtcatccagt gttttccatg tctgcttgta ttcgcgatgg
     1081 atggcagcaa tgaccgggct gatttttcct gttgaatttt cagtgtactg ttgattggct
     1141 ctggcgcggg cgagatcaac aacagacgtg tattttccgg tttccttgcg ttcaccttcg
     1201 cgacgttttt tccagatgcg catctctgcc tgaatttcgg gccatttagc accaggaata
     1261 catttatgct taacccaccc aatggcgtgc aacttaagct ccggatacat ggcgttaact
     1321 tctggcattt tcatcaacgc ttcaacgata tgtccgtcga atgttgccat gtcttcctgc
     1381 aacaattcct gtgcgctaat aaccatatca acggtgatgt tttcacatgt gtcgaactta
     1441 accatgacag cgttctgtac ttcaggggcc agcttgtcaa aagtgacgtt catcggatcg
     1501 gattcagtct caaccgggac aaaagaagca gactcctcat cccagcggtt ttcctgcata
     1561 tattcagcat cccatgaatc gagggcaggg cggggtatgc caggtttatc ctcgcagaca
     1621 ataaatttat aagcgcagtc ctgagcagcc ggataatgtt ccaggaattg ccagtgaaat
     1681 tttgcgcggg cgcgacgttc atcaccggct tcaatggcag tggctacagc gacggcacct
     1741 tcttccttta ttgcctgttc gcccggaatg gcggcgcaaa taaagacttt actcattttg
     1801 ttttaacctc atgacagatt taaggatgaa caaatccctg ccattgctgg catataagaa
     1861 tgaaaccgga tatttattac ggaactgttt taaagacctg ccggggtttc gatattatcc
     1921 tggtgaataa ctttatcgac cgggtaacag ttaccgggaa ttttctgttc ggttgctgct
     1981 gccatacatt cctgcattgt tctgtgaaca ctgactgcaa tatcaactgg ctctccggaa
     2041 acaagaaaaa ccgtcagaac aagcgcaaat gctggattca ttgtgcacat ccttttggca
     2101 tcagacgtaa acgggccagc attgaaacaa tgcatacttt atttaataac tcccgttcgt
     2161 gttttctttt gttaatggcc tcttcagtaa atacaggatt actgatagtg acaccaattt
     2221 caaaacaacc ttcagacgta ttaacgtttg gtaataacgt tttcattatc gcgccctcaa
     2281 caatgagttt tgtgatgcgg tgcctggtgc ctccaggtga cgttaaccag ttaacaatta
     2341 acgccggata cagagaatcc acccataaca ctgtttttgg ttttaactgt tccgcgtgcg
     2401 cttagccgca ttcaccgcat cacaaaattc actttaaaaa gggcggcaga gcagtcacgg
     2461 agtaaaactg ataccgccaa atgtcaccag aaaattgata acagagggcg ttgcagcggg
     2521 gttgtcactt aagcgtatgg tcaacctgac aacccggtgt cctcaacggg ggaaggaata
     2581 accccgccat acttaccgcc gcgccatttc gcggattgcc acaaccggaa gcgcacgatc
     2641 gaattaaatt taacgacgac ctatacagag agactaactt ctccgggcgc tttcgtgtta
     2701 tgccctgact tttcagggaa atatcctttc agtaaactgt cagtgccgga tgctcacccg
     2761 tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca accttagctt
     2821 cgttggttag ctattaacgc gggtatgtaa tcattctggc aatgcttaat gccgctgctt
     2881 tttccagcct ggtgatatcc tgctccagag cggacagatt ttcagcctgc ttagccctgg
     2941 cttcattggc ccatttcaga tcctgcgctg cattaatttt ctggcgcatc cactcataaa
     3001 gttcatcatc ggtatagtct ggcgcgatga tgacgggttc tcgtttctgc atactgattc
     3061 ctcgcggtgc tgcttcgctt atcagccgtt agattttgcc gagctggaaa gcgcctgttt
     3121 aaactcactg aagctgagag cttcttcgcc ttcggcaagg ccttcgaagt attcttcgta
     3181 agccttttcc atgattgtgt cgaaatccat atcactcacc tgagtttctt tccagccagc
     3241 gacgggcacc attttcggtt ttaaacgttt tgcttttggt atacgtcatc acggtgaacg
     3301 taccgtcctg gttggggaac acgccacata ccagaggttc gctgttgcca agatcgatag
     3361 tatccatgct gacctcattt ccccttaacg ccggggtagc ggaacaaaaa cctgctgcat
     3421 agttattaaa gttgaaccct gccgtcatgt tcttacgcct cgggctggct acttaacccc
     3481 tgaccactgc ctggtaactc gaagtattgc cctgcattct gtaggatggg gtgagggaat
     3541 gaatggagtt tagaaaaacg aacttttcag gtcaatgttt ttttatcaaa acattttaag
     3601 caggcagctg ttaagccatc accacgatgg catacagtta atcaaataga tgaggttggt
     3661 taaatatctt gttgaatttt aaagcatacg cccaatatgc aagatagatc atccagcata
     3721 attgaagggt agcgaggatt cgtggggact aaaagaatat ccggcccttc tatctccagt
     3781 ttacgaatga caggtgttgt ggtccctttg ggtaaggcaa ggacaatatt tcctggttgt
     3841 acggttcgat cgggatcaat aaaaactgtt gaaccatttg ggatggaaac tcccccacca
     3901 gatgttgaca tactgtcact ctctagaaca actgcaaagg tattgaccgg gatttctccg
     3961 acaagctgca cacaagaggt tattgaggaa tttttcatat aatcactcca gcttgctgcc
     4021 tgctgaagtg atagtagcgg aaccgttttt atcggcggta aagatagatc aagcgaatca
     4081 cctgtattta actctcctcc attaagaagc caattttcgt ttactttcaa tatttttgcc
     4141 agtgaactta tgtaacgcga ggacggcgct cctccaccgt tcatccattg acttacggag
     4201 ccttttgatg cgccagtgcc attgacaagg tctttgcctt tcaggtttag cgcatgcata
     4261 cgttgggtta tgcgttcaga tatagtttgc ttgctcatgt tttgatttta aaacacagat
     4321 ggttttgttt cttgactttt ttggttttga ttattaaact tttgacgttc agttttatgg
     4381 agcgactcat gaaaaaatca gaagtattag gctattttgg cggagttgtt aaaacagccg
     4441 cagctctagg aacgtcaaaa accacagtca gcatgtgggg ggaagaggtt ccgtggaaat
     4501 gggcgttgct aattcaggca gtcactgccg gggcgctcaa atatgagtta cacataccga
     4561 cggttgtcat tcccggttct gatcataatc cgccttctaa ccaagggggg gattcatgaa
     4621 aatcaagcat gaacacatcc gcatggcgat gaatgcctgg gcgcatccgg acggcgaaaa
     4681 agtaccggct gcgaaaatta ccaaagcgta tttcgagctg ggaatgacgt tcccggaact
     4741 gtatgacgac agccatccgg aagccctggc tcgcaatacc cagaaaattt tccgctgggt
     4801 agagaaagac acccctgatg cagttgaaaa aattcaggcg ttgttaccag cgatcgaaaa
     4861 ggcaatgcca cctttgctgg tggccagaat gcgcagccac agttcagctt attttcggga
     4921 gctggtggag acgcgggagc gactggtgag agacgctgat gattttgtcg cagtggcaat
     4981 cgccggtttc aatcagatga accgtggtgg cccggcagga aatgctgtgg cagtacattg
     5041 actgacaata gccatatcga atcgcttccg gcaactcgtg agtaaaaaga ttcggtatca
     5101 gaagaggtga gtatggctaa cgcttggctc agattatggc atgacatgcc aaatgaccct
     5161 aagtggcgaa caattgccag ggtgtcaggg cagccaattg caacagtgat ggcagtgtat
     5221 atccacctcc tggtgagcgc gtcacgaaat gtcacgcgag gtcacattga tgtcacgaca
     5281 gaagatttag caagtgcgct cgacgtgaca gaagaggtaa ttgattcaat tttgcagacg
     5341 atgcaggggc gggtacttga tggtgattta atcactggat gggaaaaacg ccaggtgctt
     5401 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc cggcagagcg caagagggcg
     5461 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcgc gtcacgaaat
     5521 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
     5581 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
     5641 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
     5701 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccc caaggccgga acaatcacac
     5761 caactgcagc agcctgaagc cgatttcgcc attcagcggg aagccgatcg ggtagtcccg
     5821 gaaaacaccg ggctgtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     5881 cgggagtacc cgttgcgtgc cggagcaaac ccgaagaaat ccgctttcag tgcatggaag
     5941 gccagattac gcgagggggt gccaccagag gccatgctgg atggcgtgag gcgttacgca
     6001 agatacttgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
     6061 tttggaccgg accggaattt tgagaacccc tggttgctcc cggtaagcgg cacgaacaac
     6121 cagcgttgtg tgaatcatat ttctgaaccg gataacgaaa ttccgccggg cttcaggggg
     6181 taagtgttaa tttctggtca tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac
     6241 acaagaagag cgggaaaaaa ttaaaggaca ggttgttgaa ctcgtacgcc agagtgggcg
     6301 cgagacgtta cgacaactgg aaactaaaac tggggcaaca agatatctga tgagcgttct
     6361 ggccagagag ctggttgcca gtggcgatgt atacaattct ggctacgggt tattcccgtc
     6421 tgaacaggct cgtaaagact ggcaaaatgc ccgcaaaaaa ttatcgaggg caaagctgaa
     6481 gaaaccggtt gtggttgatc cggaccttat ctggtcgtta cctgacggag aaatacgtcg
     6541 ctacgacagt cgcctaaaca taatctgtcg cgagtgccgg aagagtgaag ctatgcagcg
     6601 tgtactggct ttctatcagg gtaattttca ggaggcggta ctgtgagtga aattagctat
     6661 caggcttcaa ttaccgctgg cattcgcatc aaaggagagg agcatggaaa taaaaccaga
     6721 ggatgagtta agcaatatcg ttttatttcc ggtaaaagag gatgaccctc gtaatcaggt
     6781 taattttctt tatgagccat cggaaagacc atattgtcat cacgcctctg tccgggttga
     6841 cgaaaaagag cgtcaggtcc gctgtaaaat ctgcggtgca gttgtggagc catttgactg
     6901 gatgctctct gtggcgaaaa gagaaaccag actggcagat gatgtaaggc tcttgcgtca
     6961 ggaggagcgg gaaaggcgaa aaaatataga aaagctaatt cagattgagc gtaacgcgaa
     7021 agcgcggata cgcagggcga caaaatccag aactgaataa ttaaatttag ctctgttaaa
     7081 aatttaatcc ttaaccggag ggatttctgc accctcagaa catcaggagg ccgcccgaaa
     7141 gggcggtagt taaatgcgaa agtttaaaat aattattgaa acgggaatag ccggtggaga
     7201 tttcgaggat gaattcgaag tggatgatga tgcgacgcct gatgaaatac atgacgaagc
     7261 aaaagatatt ttctttaact actgcaatta ctcatatcac gaaataaaag acgaagagga
     7321 agaacaaaat ggctgatttt ggttcaacta aatacaacgt cagttttgaa gaatggcatg
     7381 aactgttaat ggactatgca gagttacgtg gtggcagtgc tgctgatgct gaagcatggc
     7441 gtgatgatta tgaagcagga aaaactccgg tcgaagcata ttgtgatgag tggggcgatg
     7501 aatgagcgag gttaattatc aggaagggca tgaaacggcg gggcaagcaa aaacagtggc
     7561 atggcgatat cgctacgtga aaaaaaaggc gttacggact ttcaggggaa gtagtggtct
     7621 ggtgactgga aatatgtacc gaaaaaagag gattgtaacg acaggccgaa ctatgaaatt
     7681 caggccttat tcactgcccc gccagtcccg gttacatcag aagaactggt taaagctgtg
     7741 cacttttatg aacaactaaa acgcgaaaat ccaccagcat ccggaaacct gattacagat
     7801 tcccagataa ggcaatgagc tacctggcgc agaacgggct gataagtatg gggaatgttt
     7861 tacgatgaat atttagacta aagagtttgt aacgctatgt aagtgatttt ttctggttta
     7921 gatatttata tgtccggcca aattgaggtg tgtttaaatg taattgcaca ttgattgtag
     7981 gaggaataat gaaaaacgca ttgcagtttt tgtttgttgc gttctggttg ttcgtatcat
     8041 gtatgcccat catcttcaca gcaaggtata tggaaaaagt tgatgttttg atattaatat
     8101 ttggatatat aaatgccctt tttttagggg tgttcatggc ggtcatgtgc attgaatact
     8161 ggcggtaaat acagcgaacg ccattggttt agttggatat ttactgtgcc ggacaaaaac
     8221 ggtttgcggg gaaatcttag ttaagtagaa tgactgcggg tgcttgaggc tatctgtctc
     8281 aggcatgaac accaaaaggc agatagagaa aagccccagt taacattacg cgtcctgcaa
     8341 gacgcttaac attaatctga ggctcaatct atgaacggca aatctaggtt agcctcttac
     8401 gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg
     8461 atcgtcatct gtttaaccgt catagtgacg gcactggtaa cgaggaaaga cctctgcgag
     8521 gtacgaatcc gaaccggcca gacggaggtc gctgtcttca cagcttacga acctgaggag
     8581 taagagacct ggcgggggag aaatccctcg ccacctctga tgtgtcaggc atcctcaacg
     8641 cacccgcact taacccgctt cggcgggttt tgttttttcc tggcattctg gtttacaatt
     8701 cgcacgtcag cctgaacacc tgacacctgc tgcgccagca gagaaaacag atggcgcaca
     8761 aaaccaaatt tcacaattct gataccgacc ttgccatccg gcatgagcgg cgttcacacg
     8821 catttaaaac cgactggtac caacacccac catgtactga agaacaggcc gaatggctga
     8881 ttcattctta ccgcaggcgc gggttcgagg ttaagaaagc tctcagtctc gactatcggc
     8941 actggataat ctctgtcagg ctgccttatt ccgaacgccc accacgtccg tcccgcactt
     9001 tccagcaacg gatctggagg taacgtgcgg gtattactta gacctgttct ggtgcctgag
     9061 cttgggctgg tggtccttaa gccgggccgt gaatccatac agatatttca taatcctcga
     9121 gtgctggtgg agccggaacc gaaaagcatg tgcggcctgc catccggagt cgtccctgcc
     9181 gttcgccagc cgctggcgga ggataaatca ttactgccat ttttcagcaa tgagcgtgtg
     9241 attcgtgctg ctggcggcgc tggtgcactg tctgactggc tgttgcgcca tattaaatcc
     9301 tgccagtggc cacacggtga ttatcaccac agtgaaaccg tcattcaccg ttatggtacc
     9361 ggcgcaatgg tgttgtgctg gcactgcgac aaccagttgt gtgaccagac ctcagaatca
     9421 cttgagcaac ttgctcacca aaacttgtca gcatggatga ttgacgtcat ccgtcacgca
     9481 atcagcggta cgcaggagag ggagttatcg ctggccgaat tatcctggtg ggcggtctgc
     9541 aatcaggtgg ctgatgcgct tccggagtct gtattgtgtc gttcactggg attaccggtg
     9601 gaaaaaatcc gctccgtata ccgtgagagt gacatcgtac cgggagaaca gactgccacc
     9661 agcatactga agcagcgcac aaaaaatatt gcgctgccac ttcacgtcca ccagcaacag
     9721 cccccactcc aggaaaagac gttagtaagc atcgccgttg atccggagtc tccggctcag
     9781 tatctccagc gccagaaacc acaacgggaa gagatgcctg tatacacgcg ctgggtaaaa
     9841 acgcagaaat gcatgacgtg cggtaatcag gcagatgatc cgcatcacat cattggtcat
     9901 ggcctgggag gtatgggaac aaaggctgat gatttgtttg ttattccgct gtgccgtaaa
     9961 tgtcataacg aactgcacgc cggggtaaaa gattttgaag aaaaacacgg cagccagctg
    10021 ttgttgctga ttcgtttttt aatgcacgcg agaaattcgg gtgtcctgaa gtggaaagca
    10081 tgaatgactg aacgcataga atttgttttg ccttacccgc cgacggtgaa tacctactgg
    10141 cgacgtcatg gcaatacgta tttcatctcg gaggccggaa agcgttatcg ccgtgatgtg
    10201 gcgctaattg ttcgccagca gcggctgaaa ttaaacctgt ccggaaggct ggcgataaag
    10261 gtgattgcag agccaccgga taagcgtcgt cgcgacctgg acaatatcct gaaagcaccg
    10321 ctggatgcgc tgacgcatgc gggagtgtta atggacgatg agcagtttga tgaaatcaat
    10381 atcgttcgtg gtcagccagt atctggtgga cgtctggggg tgaagattta ccccataatg
    10441 cattaagagc aggtcaaaaa atgaaactgg aagatttacc gaaatactac tccccaaaat
    10501 cccctggcct gaccgatgca tcggcctcaa cgtcaaaaga tgcgctgagt atcactgatg
    10561 tgatggccgc gcagggcatg acacagaatc gggctgagat gggtttttct gcgttcctgg
    10621 ggaaaatggg catcagtatg aatgacaggg cgcgggcaac agaattactg gcagattatg
    10681 cactcagtcg gtgcgatcgt gtggcggcgt tgagaaaact tccggcagaa ataaaaccgg
    10741 tagtgatgcg cattatggct tcgtacgctt ttgaggatta tgcccgcagc gcagcgagta
    10801 aaaagcagtg cccttgttgc tatggggaaa aatttattga aagcgtagtt tttacaaaca
    10861 aggtccagta tccggatggt aagccgccgg tatgggcaaa gtgtacgaaa ggtgtgtatt
    10921 cgtcttactg ggaagaatgg aaaaaagtca gggaggtggt aaaagttgcc tgtccggagt
    10981 gtggcggaaa gggtgaggtt tccaccgcct gtaaggattg ccgtgggcgt ggtgtcgcca
    11041 ttcattgtga agagtcggta aaacgtggta tgcctgttat cagagactgc cagcgttgtg
    11101 gtggtcgtgg ctatgaaaga ctaccatcaa cggaggcatt taatgctata tgcgaggtga
    11161 caaaccagat aacacgcgcg tcatgggaaa aaacagttaa gaaattctat gatgcgctgg
    11221 tgacccggtt tgatattgaa gaagcatggg ctgagcggca gttaaaaaag gtaactaggt
    11281 aacaaggttg atttttccgg aatctgtggt aaattcgtca taacaatggg ctttttatgc
    11341 ctgacgttag aagagtttct acaacccgcc gccgagcggg ttttttattg cggaattaat
    11401 tacggaccgt tattattctg ctcccggccc tttagctcag tggtgagagc gagcgactca
    11461 taatcgccag gtcgctggtt caaatccagc aagagccacc atcacaaacc gccattagct
    11521 tatcaggaag agcagacgac acgataacag ggttgttggt gcgggggcgg gtccccgatg
    11581 gcggtccatt atcggtattc agcgttgtta gctcagccgg acagagcaat tgccttctaa
    11641 gcaatcggtc actggttcga atccagtaca gcgcgctata ttcattcttc cagattcctt
    11701 ccggcagagc cttatactga aatatacctg gctcaggata ttgttgaaaa tattttatgt
    11761 ttgtcaaaaa taaaagttct gttaagtatt gattgagtgt ttgttatacg gtctaatggt
    11821 tttttcagca ttaaatattt atcattcata tggtgtgggt agagtgaata ttgatgaggc
    11881 gtcggggtgt ttcatcctta ggcagcgtat tgatatagtc aatgcaggac gagcaaaggc
    11941 cttcagccgt ttgacagttt tgttctgtac tcctgatcgt ctttcgggaa gagacgttat
    12001 tattctgaat agtgatgcta tacagagggt ttgcgatgag ttcatggttg ctaattcaga
    12061 attatttgct cttgttcagg agtacaacag aatagccagt acctgtggta tggatgaact
    12121 tcggattact catctggggt agatacatat ctggattatc acttgttacg gtaaaaagtg
    12181 attgcttact gtttttgtga gtggcattgc agcagccgga taatgtcagt gctggctgac
    12241 ggtgtgctgg tggcgggtgt ggtggttgtt gctttcccgt tgctgaaaaa gaaaacgcca
    12301 gactgttagc cgggtatcag ttagcgggag aaatttttaa atacttcaca attcaggcgg
    12361 ttgactgttg tctggtttgc ggggagtttg ttaaaagaaa ctggcatggt gaatccccct
    12421 gtgcggaggg gcaatcagcg agtaggtata tgggataatc gcggattcag gtgctggtac
    12481 tgaattcacc gggaggcacc cggcaccatg caatggcaca tagcgccact ctccagcccc
    12541 tctccggagg ggctgtttat attgattttg tcagatgtga gtaaactcct tatggacttt
    12601 gttgttttag tccataagga catatttgca gagtgcaacg gttattaaag cattcattca
    12661 atacgttatc tgtatttgta ggacattcct ggctgttttt gattaaattc cagaatgttt
    12721 tattgaatgg tgctacgttg taaatggtta caggtagcac tttgttattg agcatgatac
    12781 ctgtgtgagt cagtgtaaat atactttcag gaggtaagaa agcatccgat tgataccaga
    12841 ttattaattt tattttactc catatgactg aaaaagatat tccgcatgat ggctggataa
    12901 ctgtatcaat cacaatccac ttcatttagt ttccttgttt atgtcttgct ggtgatgttc
    12961 tgaaaagtat aaatgatatt tttgaatgta aaccatagag cagaattatt tttctgatgt
    13021 tgtttattgt ttatttaaat gcagggtggt ttatatctcg tcttgtagtt tatccatgca
    13081 tatctgcttg atgatgaggt ttttatttaa ggtatggttt tgtgtttttt ctgtattaca
    13141 tgtcaggtat tttaaagaat tatttttcag atggtggaaa gaaccatggc atttaaacac
    13201 tatgatgt
//
LOCUS       DAZFRE010000120        12927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFRE010000120 DAZFRE010000000
VERSION     DAZFRE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.120"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            254..1768
                     /gene="caiT"
                     /locus_tag="ACHKUI_004260"
     CDS             254..1768
                     /gene="caiT"
                     /locus_tag="ACHKUI_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6600336.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1799..2941
                     /gene="caiA"
                     /locus_tag="ACHKUI_004261"
     CDS             1799..2941
                     /gene="caiA"
                     /locus_tag="ACHKUI_004261"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6600337.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3059..4276
                     /gene="caiB"
                     /locus_tag="ACHKUI_004262"
     CDS             3059..4276
                     /gene="caiB"
                     /locus_tag="ACHKUI_004262"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6600338.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4349..5902
                     /gene="caiC"
                     /locus_tag="ACHKUI_004263"
     CDS             4349..5902
                     /gene="caiC"
                     /locus_tag="ACHKUI_004263"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6600339.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPLSANDQQHRLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6011..6796
                     /gene="caiD"
                     /locus_tag="ACHKUI_004264"
     CDS             6011..6796
                     /gene="caiD"
                     /locus_tag="ACHKUI_004264"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6600340.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6802..7392
                     /gene="caiE"
                     /locus_tag="ACHKUI_004265"
     CDS             6802..7392
                     /gene="caiE"
                     /locus_tag="ACHKUI_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6600341.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHAALHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7511..7906)
                     /gene="caiF"
                     /locus_tag="ACHKUI_004266"
     CDS             complement(7511..7906)
                     /gene="caiF"
                     /locus_tag="ACHKUI_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6600342.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8167..11385)
                     /gene="carB"
                     /locus_tag="ACHKUI_004267"
     CDS             complement(8167..11385)
                     /gene="carB"
                     /locus_tag="ACHKUI_004267"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6600343.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDRDKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11403..12551)
                     /gene="carA"
                     /locus_tag="ACHKUI_004268"
     CDS             complement(11403..12551)
                     /gene="carA"
                     /locus_tag="ACHKUI_004268"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6600344.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKCNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTIKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         3096 a         3338 c         3388 g         3105 t
ORIGIN      
        1 aaagtcagat gctatttttt tactcacaac agaacataac aaactgatta ataatcaaaa
       61 ttaacgaaaa aatgccctct attcacccga aacaaaaatg tgataacaat cacagaatgc
      121 agcttattga atacccatta tgagttagcc attaacgcgt ccacgaggtt aataataatt
      181 atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt cgttttcaca
      241 gtggaattaa ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt
      301 tccgccgtta ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcggc
      361 gaatgtcgtt attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga
      421 atggtatatg gtggtgatgc ttttcggctg gttctggctg gtgtttggcc cgtatgccaa
      481 aaagcgttta ggtaacgaac caccagaatt tagtaccgcc agttggatct ttatgatgtt
      541 cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat
      601 ctccaccccg ccgtttggct tagaaccgaa ctcaacaggc gcgaaagagt tggggctggc
      661 ttacagcttg ttccactggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc
      721 cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt cgccccagct ccaccctggt
      781 gccgctggta ggtgaaaaac acgccaaagg gttgttcggc actatcgtcg acaacttcta
      841 tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac
      901 cgagtgtatg caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat
      961 tacctgctgg attatcctca acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg
     1021 tatcgccagt gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcatcgt
     1081 cagcggtgcc agcttcatca tgaactactt caccgattcg gtggggatgt tactgatgta
     1141 tctgccgcgc atgttgttct ataccgatcc catcgctaaa ggcggctttc cgcagggctg
     1201 gaccgtgttc tactgggcat ggtgggtgat ttacgctatc cagatgagca tcttcctcgc
     1261 ccgcatctcc cgtggtcgta ccgtgcgtga actgtgcttc ggcatggtgc tggggctgac
     1321 agcgtcaacc tggatcctgt ggactgtact cggtagtaac actctgctgt tgatagataa
     1381 aaacatcatc aacattccaa atctgatcga acagtacggt gtggcgcgcg ccatcatcga
     1441 aacctgggcc gctctgccac tcagcaccgc caccatgtgg ggcttcttca tcctctgctt
     1501 tattgccacc gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt ccacttgccg
     1561 cgaagtgcgt gacggcgaag aacccccgct gctagtgcgc attggttggt cagttctggt
     1621 tggcattatc ggcattgttc tgctggcgct cggcggcctg aaaccgattc aaaccgccat
     1681 tatcgccgga ggatgcccgc tgttcttcgt caacatcatg gtgacgctct cctttattaa
     1741 agacgcgaaa cagaactgga aagattaatt aaccacccaa atatcaagag gttgaaagat
     1801 ggattttaat ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc
     1861 cagcgaaaac tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg
     1921 ttttgtcaaa gccctggcgg atatgggtat cgacagtctg ctgatcccgg aagaacacgg
     1981 tggtctggac gcggggtttg ttactctcgc cgccgtgtgg atggaactgg gtcgtctggg
     2041 agcgccaact tacgtgctgt atcagttacc gggcgggttt aacaccttcc tgcgtgaagg
     2101 tacgcaagag cagatcgaca agatcatggc tttccgcggc accggtaagc agatgtggaa
     2161 ctcagcgatt accgaaccgg gcgcaggctc cgacgtgggt agcctgaaaa cgacttatac
     2221 ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt tttattacca gtagcgccta
     2281 caccccgtac atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac
     2341 cgaatggttt gttgatatga gcaaaccggg catcaaagtg accaaacttg aaaagctcgg
     2401 tctgcgtatg gatagctgct gtgaaatcac ttttgacgac gtggaactgg acgagaaaga
     2461 catgttcggt cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg
     2521 tttcctggta gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg
     2581 ctacgccaac cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga
     2641 aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc
     2701 gtggaaagca gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg
     2761 cgccaatgcg gcatttgaag ttgtggatag cgcaatgcag gtgctgggcg gtgtcgggat
     2821 tgcgggcaac caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtctccgg
     2881 gggatctgac gaaatgcaga tcctgacgct gggtcgcgcg gtgctgaagc aataccgcta
     2941 agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc aggccgacgg taggccggat
     3001 aaggcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
     3061 ggatcatcta cccatgccga aattcggacc gctggccgga ttgcgcgttg tcttctccgg
     3121 tatcgaaatc gccggaccgt ttgccggaca aatgttcgca gaatggggcg cggaagttat
     3181 ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
     3241 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag ggcgcgaagc
     3301 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt
     3361 tgcccgtcgt ggcattaccg atgaagtcct gtggcagcac aacccaaaac tggttatcgc
     3421 tcatctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
     3481 cactatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
     3541 gcctgccttc ccgtataccg ccgattactt ttctggcctg actgccacca cggcggcgct
     3601 ggcagcactg cataaagtgc gtgaaaccgg caaaggcgaa agcatcgaca tcgccatgta
     3661 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
     3721 gtgcccgcgc atgaccaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg
     3781 cgccgacggc tacatcgtga tggaactggt gggcattact caaattgcag agtgctttaa
     3841 agatattggc ctcgcacacc tgctcggtac gccggaaatc ccggaaggca ctcagcttat
     3901 ccaccgtatc gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc
     3961 ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
     4021 agtactgacc gtgccggaac tggaaagcaa tccacagtat gtggctcgcg aatcaatcac
     4081 tcagtggcaa acgatggatg gtcgcacctg caaagggccg aatatcatgc cgaaattcaa
     4141 aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
     4201 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc
     4261 caaagttgag gactaaacgc gtggcgtcag tcaacttgcc tgactagcgc ccgcccaata
     4321 acaaacgaac aaaatggata gaggtgcaat ggatatcatt ggcggacaac atctacgtca
     4381 aatgtgggac gatctggcgg acgtttacgg tcataaaacg gcgctgattt gtgaatccag
     4441 cagcggagtc gttaaccggt atagttatct tgagttaaat caggagatta accgcacggc
     4501 aaacctgttt tatacgctgg ggattcgcaa aggcgacaag gttgctctac atctcgacaa
     4561 ctgcccggaa tttatctttt gctggttcgg gttggcaaaa attggcgcga ttatggtgcc
     4621 gattaacgcc cgcctgttgc gcgaagaaag cacgtggatc ctgcaaaata gccaggcgtg
     4681 cctgctggtg accagtgcgc aattttatcc tatgtatcaa cagattcagc aggaagatgc
     4741 ctctcagttg cggcacattt gcctgacaga tatggtactt cccgctgatg atggcgtgag
     4801 ttcgtttact caactgaaaa atcaacaacc tgccaccttg tgctatgcac cgccgctatc
     4861 gactgacgat acggcggaaa ttctcttcac ctccggcacc acctcccgac cgaaaggggt
     4921 ggtgataact cattacaacc tgcgcttcgc tggatattac tccgcctggc agtgtgcact
     4981 gcgtgacgat gacgtctacc tgacggtaat gcctgcgttt catatcgatt gccagtgtac
     5041 tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga aatacagcgc
     5101 ccgcgccttc tggggacagg tgcagaagta ccgcgccacc attaccgaat gtattccgat
     5161 gatgattcgt acgttgatgg tgcagccgct ttcagcgaac gatcagcaac accgcctgcg
     5221 ggaagtgatg ttttatctca acctgtcgga gcaggaaaaa gacgcgtttt gtgaacgctt
     5281 cagcgttcgc ttgctgacgt cttatgggat gacggaaacc attgtgggca ttatcggcga
     5341 tcgccctggc gataaacggc gctggccgtc gattggtcgg gcgggctttt gctacgaagc
     5401 ggagatccgc gacgatcaca atcgtcccct cccggctggt gagctcggtg aaatctgtat
     5461 taaaggcgta ccagggaaaa ccatcttcaa agagtatttt ctcaacccga aagccactgc
     5521 gaaagtgctg gaagccgatg gctggctaca taccggcgat accggatacc gcgacgaaga
     5581 gggctttttt tatttcgtcg atcgccgctg caatatgatc aaacgcggtg gcgagaatgt
     5641 ctcctgcgtg gagctcgaaa atatcatcgc cacccatcca aaaattcagg atatcgtggt
     5701 tgtgggtatt aaagattcga ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga
     5761 aggtgaaaca ttgagcgaag aggaattctt ctgcttctgt gaacagaata tggcgaaatt
     5821 taaagtgccc tcttatctgg agatcagaaa agatctgcca cgtaattgct cggggaaaat
     5881 aattagaaag aatctgaaat aaatgaaacg gcagggaact accctgtcag ctaataatca
     5941 cgctctaaaa caatatgcat tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg
     6001 gagaaaagga atgagtgaat cattacatct gacccgcaat ggatcaattc tggaaattac
     6061 ccttgatcgt ccaaaagcga atgctattga tgcaaaaacc agctttgaaa tgggcgaagt
     6121 atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc attattaccg gtgccggaga
     6181 gaaattcttt tccgcgggct gggatttaaa agcggcagca gaaggcgaag caccggatgc
     6241 tgactttggc ccgggaggtt ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt
     6301 catcgccgct gtgaacggct atgcctttgg cggcggcttt gaactggcgc tggcggcaga
     6361 ttttattgtt tgtgccgata acgccagctt cgccctaccg gaagccaaac tgggcatcgt
     6421 tcctgacagc ggcggcgtgc tgcgtctgcc gaagatcctg ccgcctgcca tcgtcaatga
     6481 aatggtgatg accggcagac gaatgggcgc agaagaggcg ctgcgttggg gggtagtcaa
     6541 ccgcgtggtt agccaggcgg aactgatgga taacgcccgc gaactggcgc agcagctggt
     6601 taacagcgct ccgctggcga ttgcggcgct gaaagagatc taccgcacca ccagtgaaat
     6661 gccggtagaa gaagcgtatc gctatattcg cagcggcgtg ttgaaacact atccgtcggt
     6721 tctgcattcg gaggatgcca ttgaagggcc gctggcgttt gccgagaagc gcgatccagt
     6781 gtggaaagga cgttaacaac cgtgagctat tacgcctttg aggggttaat tccggttgtt
     6841 cacccgacgg cgtttgtcca tcccagtgca gtattgattg gcgatgtgat tgtgggagcc
     6901 ggtgtctaca tcggcccact cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa
     6961 gcgggagcca atattcagga tggctgcatt atgcatggct actgcgacac tgacaccatc
     7021 gttggggaaa acggtcatat cgggcacgga gcgatcctgc atggttgtgt gattggtcgc
     7081 gatgcactgg tcgggatgaa cagcgtgatt atggatggcg cggtcattgg cgaagagagc
     7141 attgttgccg ctatgagctt tgtcaaagcg ggctttcgcg gcgagaaacg ccagttgttg
     7201 atgggtacgc cagcccgcgc tgtacgcagc gtgagtgacg acgagttaca ctggaaacgg
     7261 ttgaatacca aagagtatca ggatcttgtc gggcgctgtc atgcagcgtt acatgaaacg
     7321 cagccactga ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg
     7381 ccgaaacggt aacatcgtcg gatgcggcat aaacgcctta tccgacctgg ctgtaaagca
     7441 accgtaggcc ggataagatg cgccagcatc gcatccagcg accaggtcgc acggtatcaa
     7501 cgaaatgaca ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt
     7561 taagttccgt gagcggaacg gcaggaatac gcggcgtctt tcttacgccg actaatttat
     7621 cccggctgtt attgcgcagg cgcgcgtaaa tttgctcatc gatatcaacc acttttacca
     7681 gtcgctggca ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc
     7741 ttatttctgt gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa
     7801 tatcgaaatg aatagagatc tctcttgcta tcacccaccg gttttcagcc atcatccatt
     7861 cggcgattaa caaatagagt ggtttttcaa cacatccttc acacatacga actccagttt
     7921 cacaaattaa aagactggct atgactcgtt ttgcagcaat taaacaaggc catattaaaa
     7981 ttgtggatat atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt
     8041 atgtcatcaa aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaacggtcct
     8101 catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccatgac
     8161 acgctattat ttgatctgcg cgtgcatttc ctgcaccgaa attacttttt cggtcgcatc
     8221 ggcattcagc gccatcgcgg tggcaaagcc gccgttcagg gtggtgtcgt aatgcacttt
     8281 atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac gacctgaggt
     8341 ggtgttgatg atgtaggtat attcgccatt cttaatacgg tcctgaatgt gcggacggcc
     8401 ttcatgcacc ttgttcacca gacgcggatt gatacccgct tcgcccagca caatcgccgt
     8461 gccgtgagtc gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac
     8521 gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga
     8581 gttgctgccc agctgcgctt tggcaaacgc ttcagcgaag gtgcggccca cgcccatgac
     8641 ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcccggga atttattgaa
     8701 cggcagcacc acttctttca ccgagtagta cggcgggata acttctttgg ttacgccctg
     8761 ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt
     8821 ggctttggag acaaacggaa cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac
     8881 ttcgttgttt ttcaccgcaa actgcacgtt catcaggccg cgcacctgca attcgaaggc
     8941 cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtaggctgg
     9001 cagcgaacat gcggagtcac cggagtgcac gcccgcctgc tcgatgtgct ccatgatgcc
     9061 gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc
     9121 aaggaagtgg tccagcaaca ctggcgcatc gttagatacg ctgaccgctg tctggaagta
     9181 gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga
     9241 cggacgcacc accagcgggt agccaatctc tttcgctttc tcaaccgcca tttcgatggt
     9301 ggtaacggtg gcgttcgccg gttgtttcag tttcagacgc tcaaccgcat gctggaagcg
     9361 ttcacggtct tccgcacggt cgatggcatc cgggctggta ccgataaccg gtacgccagc
     9421 agcttccagc gcgcgcgcca gtttcagcgg ggtctgaccg ccgtactgga cgataacgcc
     9481 tttcggcttc tcaatacgca caatttccag cacgtcttcc agagttaccg gctcgaagta
     9541 gaggcggtcg gaagtgtcgt aatcagtgga gacggtttcc gggttacagt taaccataat
     9601 ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acacagcagt agtcgaattc
     9661 gataccctga ccgatacggt tcgggccgcc gccgaggacc atgattttgt cacggtcaga
     9721 tgggttggat tcgcactctt cttcataagt ggagtacatg taagcggtgt cggtggcgaa
     9781 ctctgccgca caagtatcca cgcgcttgta aaccgggtgc agatcatact ggtcgcgcaa
     9841 cttgcggatt tccgcttcgc gcacgcccgc cagtttagcc agacgcgcat cggcaaagcc
     9901 tttgcgtttc aactggcgca ggaattcagc gtgcaggcca gtgatgccca cttctgcgac
     9961 tttctcttcc agacgcacca gctcttcaat ctgtaccagg aaccagcggt caatgttggt
    10021 caaattaaat acaccgtcta cagacaggcc cgcgcggaag gcatcagcga tataccagat
    10081 acgctctgcg ccagcatctt tcagttcgcg gcggatttta gtcagcgctt ccgggtcgtc
    10141 gagactcact ttcggatcga agccagttgc gcccacttcc aggccgcgca gcgctttttg
    10201 tagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt tcatctgagt
    10261 ggtcagacgg tcgttagcac cggcgaattt ctcgaagttg aagcgaggaa ttttagtaac
    10321 cacatagtcg atggacggct cgaaggaggc cggagtacgc ccaccggtga tgtcgttcat
    10381 cagctcgtcg agggtgtaac ccaccgccag tttcgccgcc actttggcaa tcgggaaacc
    10441 agtggctttc gacgccagcg cagaggagcg agacacgcgt gggttcattt cgataacaat
    10501 caggcgaccg tttttcgggt tcaccgcaaa ctggacgttg gaaccaccgg tttcaacgcc
    10561 gatttcacgc agtaccgcca tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag
    10621 cgtttgggct ggcgcgacag tgatggagtc accggtgtgg atgcccatcg catcaaagtt
    10681 ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt ccatctcgta
    10741 ctctttccag ccgatcagcg actcatcaat cagcagctct ttagtcggag aaagatccag
    10801 accgcgggcg caaatttctt caaattcttc acggttataa gcgataccgc caccgctacc
    10861 gcccatggta aaggatgggc gaataatgca cgggaagcca acttcagcgg caaccgccag
    10921 cgcttcttcc attgaatgag cgataccgga acgcgcagtt tccagaccga ttttcttcat
    10981 cgctacgtcg aaacggcggc ggtcttcggc tttatcaatc gcatcggcag tggcaccaat
    11041 catggtgacg ccgaactctt ccagcacgcc ctgacgctca agctccagcg cacagttcag
    11101 cgccgtctgc ccccccatgg ttggcagcac cgcgtccggg cgctcttttt caataatctt
    11161 gcgtaccact tcccagtgaa tcggctcgat gtaggttgca tcagccattt ccgggtcggt
    11221 catgatagtc gccgggttgg agttcacgag aatgacgcgg taaccctctt cacgcagggc
    11281 tttacacgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc
    11341 cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc ttttactcct
    11401 gattacttag cggttttacg gtactgctca attaacgcga taaagtggtc aaacaacggc
    11461 gcggcgtcgt gtggaccagg gctggcttcc gggtgtccct ggaagctgaa cgccggttta
    11521 tcggtacgat gaatgccctg taacgtaccg tcgaacaggg atttatgtgt gacacgcagg
    11581 tttgcaggta atgttgcttc atccaccgca aaaccgtggt tctgggcggt gatcatcacc
    11641 acatttttct caacatcttt aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg
    11701 atggtcttcg caccgctcgc cagcgccagc agctgatgac cgaggcagat gccgaatacc
    11761 ggaatatcag tttcgaggaa tttctggata gcggtaatgg cgtaatcgca cggggccggg
    11821 tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac atcttccgca
    11881 gaagtttgcg ccggaacgat ggtcaggcga cagcctctat ccaccagcat acgcaggatg
    11941 ttgcacttgg caccgaaatc gtaggccacg acgtggtacg gcagctcgtc ttcttttttc
    12001 gcttctggca ggccgccggt cagtgtccag ctcccttgtg tccagctata gggttctgcg
    12061 gtggttactt cttttgccag atccatgccg ttcagacctg ggaacgcgcg ggctttttct
    12121 aacgccagcg ccgcatccgg gttgtcaccc gcaataatgc agccattctg agcacctttc
    12181 tcacgcagta aacgcgtcag cttacgggta tcgatatcgg caatcgccac gatgttatgg
    12241 cgcttcaggt aagaagagag gtcttcggta ttacggaagt tgctggcaat cagcggcagg
    12301 tcgcgaatca ccagaccttg ggcatgaacc tgagaagatt cttcatcggc gtcattagtg
    12361 ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata ggaaggatca
    12421 gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa
    12481 cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac caatagcgct
    12541 gacttaatca aaacaccctc cagagaatat tcactcactt tatttgcata ttaattcaca
    12601 atgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa cggcggcatt
    12661 ctggagatat gggagctaaa agtcaactta atggcgggat tttttatatt attttgtggc
    12721 tcatgcatgt tttacaggca ttaagcgaca aaaagatcat ataacatgtt ctggaaaacg
    12781 cttgcgcagc aagaagattg catgaaatca aaatgagcag aaaaaaatgg accaaatggt
    12841 caaaatttac attaaaacaa caaaaacaga atacaaaaca tgtaattttc agcatttgca
    12901 ttagatttaa aatcataaca ctaaaaa
//
LOCUS       DAZFRE010000121        12868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFRE010000121 DAZFRE010000000
VERSION     DAZFRE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.121"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..328)
                     /locus_tag="ACHKUI_004269"
     CDS             complement(<1..328)
                     /locus_tag="ACHKUI_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6600345.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWE"
     gene            complement(423..716)
                     /locus_tag="ACHKUI_004270"
     CDS             complement(423..716)
                     /locus_tag="ACHKUI_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600346.1"
                     /translation="MFLGFVWRITKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLASVIVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRAR"
     gene            864..1826
                     /gene="parM"
                     /locus_tag="ACHKUI_004271"
                     /gene_synonym="stbA"
     CDS             864..1826
                     /gene="parM"
                     /locus_tag="ACHKUI_004271"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6600347.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHSQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            1829..2179
                     /locus_tag="ACHKUI_004272"
     CDS             1829..2179
                     /locus_tag="ACHKUI_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6600348.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            complement(2344..2499)
                     /locus_tag="ACHKUI_004273"
     CDS             complement(2344..2499)
                     /locus_tag="ACHKUI_004273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600349.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KQFPFFF"
     gene            3320..4558
                     /locus_tag="ACHKUI_004274"
     CDS             3320..4558
                     /locus_tag="ACHKUI_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO6600350.1"
                     /translation="MEVFMKDVSILNEMIFSASTENELQEKFEDIYHRINNESEYDDS
                     ESVEFLPLPRLNSRSERSEVQDIISVVIKSGCFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHQIARKADIPVIVDAAQCFGIRSLISHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSRMDN
                     LQAATLSVKLQHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPVLLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFRDSEKIHSGLIHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            4542..5372
                     /locus_tag="ACHKUI_004275"
     CDS             4542..5372
                     /locus_tag="ACHKUI_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFO6600351.1"
                     /translation="MTILNKNWPVEFPFPTVIRTVVCSDLDETYIPSDSDKKKLGGVV
                     QLESYITSHAEKKGILAGWITGTNLDSAWRKSRGYISRSPHFICCSLGTEFYWVKNGR
                     LIPSETWAERISRSGYSRQNVNCLVKILMEKEIPLLKQPEDYQGPFKVSYYYPESPSI
                     ASDFATIQELADERRIRVVFTRCNPAAGDPPGCYDVEFIPLCCGKDQAVSFLMEETGL
                     PKEAIIAFGDSANDFAMFAQAGSGYLVGNADPFAVSQYGNRLDSAYCHGILSVLKELQ
                     "
     gene            5372..6388
                     /locus_tag="ACHKUI_004276"
     CDS             5372..6388
                     /locus_tag="ACHKUI_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6600352.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVSPDNVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            6390..6659
                     /locus_tag="ACHKUI_004277"
     CDS             6390..6659
                     /locus_tag="ACHKUI_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFO6600353.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            6656..8188
                     /locus_tag="ACHKUI_004278"
     CDS             6656..8188
                     /locus_tag="ACHKUI_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6600354.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESTRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPLAERFTGEGAIEFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(8220..8672)
                     /locus_tag="ACHKUI_004279"
     CDS             complement(8220..8672)
                     /locus_tag="ACHKUI_004279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6600355.1"
                     /translation="MKFMECAVRDVIYGTNVRLVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPIENKGIYAGNPARLLRIL"
     gene            9002..9250
                     /locus_tag="ACHKUI_004280"
     CDS             9002..9250
                     /locus_tag="ACHKUI_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600356.1"
                     /translation="MYEDMLFSLSYEQMTQMAEEEIKQCDFRRDGTHYVWEVNKAHDI
                     LRFWYLLALRGHTGLATTRVEADYKRLKTLISQRNEGQ"
     gene            9247..9843
                     /locus_tag="ACHKUI_004281"
     CDS             9247..9843
                     /locus_tag="ACHKUI_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600357.1"
                     /translation="MMREKARRPSPLQRRVLIVLAALDEKRPGPVLTRDIERGLERSG
                     EAPVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAE
                     QRAAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNK
                     EGRVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(9861..10208)
                     /locus_tag="ACHKUI_004282"
     CDS             complement(9861..10208)
                     /locus_tag="ACHKUI_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6600358.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFWFGLLMASFHWW
                     RRKVNRLPPWDNV"
     gene            complement(10509..10991)
                     /locus_tag="ACHKUI_004283"
     CDS             complement(10509..10991)
                     /locus_tag="ACHKUI_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600359.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG
                     "
     gene            complement(11108..11956)
                     /locus_tag="ACHKUI_004284"
     CDS             complement(11108..11956)
                     /locus_tag="ACHKUI_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO6600360.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMAAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(12002..12280)
                     /gene="yacB"
                     /locus_tag="ACHKUI_004285"
     CDS             complement(12002..12280)
                     /gene="yacB"
                     /locus_tag="ACHKUI_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO6600361.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(12280..12549)
                     /gene="yacA"
                     /locus_tag="ACHKUI_004286"
     CDS             complement(12280..12549)
                     /gene="yacA"
                     /locus_tag="ACHKUI_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO6600362.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRDEMSRKVADK"
BASE COUNT         3427 a         3222 c         3027 g         3192 t
ORIGIN      
        1 cttcccagcg acgctgtcca gtaccgctct gcagccagag ttcatgcagc atcaggcagt
       61 tttgctcatc cagcagtaca ccgttctgca ggtgtgtcag ccagcgggtg aacgcgatat
      121 tttccggcga tacagcacca cgtccggcag ccgtcactcc ggggcactgc tcagagcgga
      181 tattttcagt gatctgcaga agtccggcac ttccggccag aaacagctga ataccttcat
      241 ttgtggcgcg gcgataaagc ggtgtgactg caccgctggc cagtcgttta atctcagcga
      301 tctgtgccgg ttcaccagaa aaatacatac gattcgagca ccagtttggc atgggaatta
      361 tcctcacagg agcatcggga gggcgcccct gtggcgcccg ttccccgctg cctgtgggct
      421 gttcaccgcg cccggtaacg ggtcaggccg accgtggaat accgcagccg tcgtcttttt
      481 gacggcaagg ctatgccgcg aaagcggctt gaagggcgct catcagaaaa gggcgacggg
      541 ctggcgtaca ctatcaccga cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgg
      601 ccggacgcgg gacactgacc acaggcagcg ttcctgccgc gccgcgtcag ccggcgcagt
      661 aaaaagagga gcccggcgac atcttttgtt atccgccaaa caaaacccaa aaacaaccca
      721 taccaaaccc aacaaaacac caaaacaaga caaataatca ttgattgata gttaaaatgg
      781 ggtaaacttg acaaataaac ccatttaaag cccaaaacac acccaaacaa acaccaaaaa
      841 aaacaccata aggagtttta taaatgttgg tattcattga tgacggttca acaaacatca
      901 aactacagtg gcaggaaagc gacggaacaa ttaaacagca cattagcccg aacagcttca
      961 aacgcgagtg ggcagtttct tttggtgata aaaaggtctt taactacaca ctgaacggcg
     1021 aacagtattc atttgatcca atcagcccgg atgctgtagt cacaaccaat atcgcatggc
     1081 aatacagcga cgttaatgtc gttgcggtgc accacgcctt actgaccagt ggtctgccgg
     1141 taagcgaagt ggatattgtt tgcacacttc ctctgacaga gtattacgac aggaataacc
     1201 aacccaatac ggaaaatatt gagcgtaaga aagcaaactt ccggaaaaaa attacattaa
     1261 atggtgggga tacattcaca ataaaagatg taaaagtcat gcctgaatct ataccagcag
     1321 gttatgaagt tctacaagaa ctggatgagt tagattcttt attaattata gacctcgggg
     1381 gcaccacatt agatatttct caggtaatgg ggaaattatc agggatcagt aaaatatacg
     1441 gagactcatc tcttggtgta tctctggtca catctgcagt aaaagatgca ctttctcttg
     1501 cgagaacaaa aggaagtagc tatcttgctg acgatataat tattcacaga aaagacaaca
     1561 actatctgaa gcaacgaatt aatgatgaga acaaaatatc aatagtcacc gaagcaatga
     1621 atgaagcact tcgtaaactt gagcaacgtg tattaaatac gctcaatgaa ttttctggtt
     1681 atacgcatgt tatggttata ggcggtggcg cagaattaat atgcgatgca gtaaaaaaac
     1741 actcacagat tcgtgatgaa cgttttttca aaaccaataa ctctcaatat gatttagtta
     1801 acggtatgta tctcataggt aattaataat ggacaagcgc agaaccattg ccttcaaact
     1861 aaatccagat gtaaatcaaa cagataaaat tgtttgtgat acactggaca gtatcccgca
     1921 aggggaacgc agccgcctta accgggccgc actgacggca ggtctggcct tatacagaca
     1981 agaccccagg gcccctttcc ttttatgtga actgctgacg aaagaaacca cattttcaga
     2041 tatcgtgaat atattgagat cgctatttcc aaaagagatg gctgatttta attcttcaac
     2101 aatcactcaa ccctcttcac aacaggagca aagaagtgat gaagagacaa aaaaaaacgc
     2161 gatgaagtta ataaactgat taaattttta ttgatatctt tacccccata aacggggtaa
     2221 aggtatcaac aaacctattt tttagctatg tacagattac atctgacaag ttatttctac
     2281 gtaacaaatc acagtctaaa aatcagtata gctttaccct ttgcactgtt cagcttattc
     2341 ctttcagaaa aaaaacggga actgttttgc cgcatccggg acaagcagcc tggctgcgcc
     2401 ggcattccag ctgaggtaga catatccggc ctgcgcctgt acagactgcc agtaatccgt
     2461 tccggcgaca gcctgacctt cattctcaat aaaaaacatg ggtgactcct tttttctttt
     2521 ccgggcaggg acggtcatgc gataacgtca tccatcatca ctgcccttac cgtcacgccg
     2581 tctgataaca tcatgccgtt atcatcatca ccatgataac aagcatccat ataaccacgc
     2641 cgttaaatta ccggagagcc gtttccgtca gatccgactt tccggaatac cacaactttc
     2701 cggaagtgta ccacgctgag aaaatcatca tatcgctgtt ttccgcccct gcgcggcgca
     2761 tcacaggtca gggagatgac acaacatata gtgtcatcag cagctgaatt cagcactaca
     2821 tgttgtgctg acgggagatg gcgctgtgcg gagggagagg aagatccgta acaataagtt
     2881 ttcggatctc tggatcagaa aagaaaatta ttgccagata atcacaaaaa atggtgaatt
     2941 attgtgatga ggtgattatt gcaatcagaa caataactgg aattgaaaag gttatattca
     3001 tcaataaata gcagagtcac agacctgctg taaagccaga ccatgaagat actacccgaa
     3061 cctgcttcag gaagatccat ttcaaatatc cggatacaca ggcaactgtg acgacaattg
     3121 taatgacatt aaccatatcg gcttccctga agcattggtt atggcaacca cgttatcaat
     3181 acctttccat cgttttcagt aaatgtcact aacagaccga tattaccgga cctgtaagtt
     3241 gtctgtgacc aatgtcacca atttgtaact caaatgtgaa aatatggttt atctgtgctt
     3301 tatttttctc cgacataaaa tggaggtctt tatgaaagat gtcagtattt tgaatgaaat
     3361 gatcttcagt gcatcaacag aaaatgaact acaggaaaaa tttgaagata tatatcaccg
     3421 tatcaacaac gagagtgaat atgatgattc ggagtcagtc gaatttctgc cacttccaag
     3481 actgaattcc cgttctgaac gctcagaagt acaggacatc atttcagttg taattaaaag
     3541 cggatgtttt accagtggcc cctatattgg aaaagtagaa gagacattaa gattatttta
     3601 tggtgctcat acctgtattg ccaccagtag cggcaccgat gcgttaaaaa tagctcttaa
     3661 atctgttgga gtggaacccg gagatgaagt catcgttccg ctgaactcct ttgcagctac
     3721 ggagaatgcc gtgatggccg tgggagccgt tccggtcttc gctaatattg acagttcttt
     3781 caatatgctt ccggatgaag tggatcgcct cagaacttca aagacaaaag ccgtgttacc
     3841 ggtctgcctt tatggctcat gcatgtacat tgatgccatt caccagattg cccgcaaagc
     3901 cgacattcct gttatcgtgg atgcagcgca gtgctttggt atcaggtcac taatcagcca
     3961 ctgtgatctg ctggcgttga gttttaatcc atttaagaat attggttctc ttggtaaatc
     4021 cggtgcagtt ctgacccttt ctcctgaact ggcacgtctg gcaaggcaat attcatatca
     4081 cggatttgct gaaggtaaaa agaatattaa ggcccagaac tggggattaa acagtcgtat
     4141 ggataatctg caggctgcca cgcttagtgt caaacttcaa cattttgaaa ataatgcaaa
     4201 aaaacgctgc cttcttgcag cccgttacca tcttttactg tctgacctgt cacataacat
     4261 aatacttccg acagagaccc atcagaatac atggcacctt ttcccggtac tgctctgcaa
     4321 aggcgaacgt gattctctgt tcgcctttgc tcgtgaaaaa ggagtggagt tagatattta
     4381 ctatcccgtc ctttcacact gcggagagca tccattggca accggatatt cccgtcgaga
     4441 gcagttccgt gattctgaaa aaatacattc tggattaatt catctgccgc tgcataacca
     4501 tatgtcgctc caggagcaaa acatcgttat cgaggtactg catgactatt ttaaataaaa
     4561 actggcccgt cgaattcccg ttcccgacag taatccgcac tgtggtgtgc agcgatcttg
     4621 atgaaactta tattccttcg gacagtgata aaaaaaaact ggggggggtc gttcagctgg
     4681 agtcctatat tacatcccat gctgaaaaaa aggggattct cgctggctgg atcacgggaa
     4741 caaatctgga ttcagcctgg cgaaaatcca ggggatatat ctcgcgaagc cctcacttta
     4801 tatgttgcag tctcggcacc gaattttact gggtgaaaaa tggccgtctc atcccctctg
     4861 aaacctgggc agaaagaata agccgttcag gctacagccg tcagaatgtc aactgtctgg
     4921 ttaagattct gatggaaaaa gagatccccc ttctgaaaca accagaggat tatcagggcc
     4981 cctttaaagt gagctattac taccctgaga gcccatctat tgccagcgat tttgccacca
     5041 ttcaggaact ggctgacgaa cgacgaattc gtgttgtgtt tacccgctgt aacccggcgg
     5101 ctggcgatcc ccctggctgt tatgacgttg aatttatccc cctctgttgt ggcaaggatc
     5161 aggctgtgtc ttttctgatg gaagaaaccg gactgccgaa agaagccatt attgcctttg
     5221 gtgacagtgc gaatgatttt gccatgtttg cacaggccgg aagtggctat ctggtgggta
     5281 atgccgaccc gtttgcagtc agccagtacg gaaacagact ggacagtgct tactgccacg
     5341 ggatactgag cgttcttaag gagctacagt gatgattatc ggaattgtcg gctgtggggg
     5401 gattgcccgt gcccatgtca acgcactttg tctcaatccg ctagtaactg gtctggccct
     5461 gtacgatgtg agtccggaca acgtccgaca gctcagcgaa ataagctccc tgccggtaac
     5521 aaactgccag gatctggagc agcttgccgg agtcagtcaa gggttcatta tctgcacacc
     5581 aaaccatctc cacgttagcg tggcagaaga agtgcttcgc tacaaacagc tcccgtttgt
     5641 gtgtgaaaaa cccctctcaa cagatctcaa ctctgcacga tatctacagg agattgctcc
     5701 aaaaggtagt atcgtgtctt ttaactaccg atataacccc gtcatcgaca tgataatgcg
     5761 tctgagaaat gagaggaaat taggtgaact gcatttcttc tcagcagaat ttaataaaaa
     5821 ttctgcactg acccgccatc acctgacctg gcgagactcc gcacaacaga gcaaaagcag
     5881 cggtgctctg ggcgatctat cctgtcatct gcttgattta ttttgtttta taggggaaag
     5941 cccggtagtg gttcatggga tcaaaacggt aaaggggaca cgggtgggta caaaatcaga
     6001 tggtcaggtt gaagtggatg ataacggcta tgtaatgggc agttcagaaa agggagctta
     6061 tttcagagtt catgccagta aatcggaaac agatcagaat ttgggactgc atatacagct
     6121 tgtatttgaa aatggtgaaa tcagatattc aacccaccat gaaaatcgcc tgctcctgat
     6181 tctatttaat gatacgaata cggaaacaat tggcttcgac gctctgaaac gcttgcctga
     6241 tccaccacgg gagcttccat tctggtctga ttcgtttatt catcttcatg acgactggtg
     6301 tgctcttatt aaatatggtc actcgtctcc taagctggcg gacctgtcat ccggccttca
     6361 tatacaggaa ataattgagg cattctgata tggatacaat tcttttaacc gggctgtttg
     6421 ctgcattttt cacaacgttt gcgttcgctc cccagagcat aaaaactatc aggacacgca
     6481 ataccgaagg tatctccgtt gttatgtata tcatgttcct gacaggtgta atctcatgga
     6541 tagcgtatgg cattatgcga tctgattttg ccgtactgat cgccaatatt gtcacactgt
     6601 ttctggctgc accagtgctt gtaataacac ttatcaaccg caggaaaaaa cacgtatgaa
     6661 tattgcaatc atcggcgcgg gtcctgccgg catcatttct gcccgtaatg cgattaaagc
     6721 aggtcactcc gtggttctgt ttgagaagaa tacccgaatt ggggggatct ggaacccctg
     6781 gagtggtggc gcttaccgta atgcctgtat gcagaactcc cgttatacat ttcattatac
     6841 tggctttccc cctggcgata tcgatgaatt tccaggagtg gaacaggtat tcagatatct
     6901 ttcggccgtg gccggagagg atgccctccg tgagtcgacc cgactgaata ctgaagttgt
     6961 ttcactcaga aaagacgccg gacactgggt gatccgctgc gcttctgaag gaaaagacac
     7021 ggaagacatt tttgaccggg tcatcattgc aacaggtgaa ctctggcaac cccgccggcc
     7081 ccccctgcca ggtgaggaaa acttctccgg aacgttgatc acgtcgagag attatcagga
     7141 gccagaggca tttaaaggaa aaaatatcct catcattggc ggcggtgtca gcggtgcgga
     7201 tattgcctca gaccttgttc cctttgccag aagcgtaagt ctgtccgtca aaaagatggg
     7261 actttatctg ccgagacaat tcccgactgg cccgaatgac atgatgcact cctatctggg
     7321 caggtgtctg ctgagccaaa tgaattatga agactttatc ggttatctcg acaccatgat
     7381 gcctgactac atgcaggcct accgagcatc cggtctgttg cccgacatgg cgaacaataa
     7441 cgcggtacat gttaacgaaa aaatcatacc gaatgtggcc gccggtctga taaaagttaa
     7501 acccctggca gagcgtttca ccggagaagg cgctattgag ttcgtcgacg catctcagga
     7561 aaaatatgat gtaatcataa cctgtacagg ctatgaaatg cctgattact cgttcattca
     7621 gggatttgac agaacacagc tctatgagca ctttttctgg acagaggacc cttctctggc
     7681 cgtcattaat cccccggtgg ataccgccgg tttcggggct gcgtttccct attttgatat
     7741 catcagccag tgggtaatga acgtattctc cgggaagact tcccttccgg aaaaagaagc
     7801 tatgcgcaaa tggtgtgctg agcacatggc ttcacttcat gttaagcgat tttatgacag
     7861 ctggctggaa accatccgga ttgggttgct gtcaggtctt ctcccggacc cggcccgcga
     7921 cttttcccga tactggaaca tcatcagcag tatggtcaaa cctgcctatc tggccactcc
     7981 tcccgcattt ccggagcatg gtatgatgga cagcctgttt gatttccgga tcgccagaat
     8041 acgcatactg tcagggctgg gaaacgatgc tctcggttat ttactgaaaa aaggtgatat
     8101 cactgacgca gaataccggg cggccctgga aatcgatccc cggcagtcga tttctgtaca
     8161 cctgccgtat tcacaaacct acctctgata aaaagggcca gtaacggccc ttttcttttt
     8221 cataatattc tgagcagtct tgccgggttt cctgcataaa tgcctttgtt ctcaatcggt
     8281 tttactacaa ctgcccccgc accgataaca gctccgctgc atatatacgg agacagaata
     8341 atggctccac tgccaacagt aaccgaatcc cccaggataa tgctgatcca gttatccggt
     8401 gacggatctg gtgctcctga acgaaacaga tcgttggcga acgtcacgtt atgaccaatg
     8461 aagcagttct cccccagtgt tacgttctca caaataaacg tgtgcgactg tatccgactt
     8521 ccactaccaa tgacacagcc tttctgtatc tcaacgaagg ggccgacaaa cacattgtcc
     8581 ctgagttcac attcataaat gttgaccggt ttgacaagac ggacattggt cccataaatc
     8641 acatcccgga cagcacattc cataaatttc atactcacac gtcctttaaa tacagatgac
     8701 acttgccgga attatacagc ccagattcag ttgccatgtt tcagaatagc ttataagccg
     8761 cacttttacc gatactcagc aacaaatcag gtctggatcc acttcagcca gaaaccggat
     8821 ctgcgcggcg ggaaacgcgc aaaggatatg ataatcaaac cacacacata aaatcatact
     8881 gaatgtgata tttagcatta aacgcataat gatatctgat atgatatttg aattatgagt
     8941 aagaaatcat acgtgatatg atttttaacc taaccggaca tgttcagacg gagtgtgcag
     9001 gatgtatgaa gatatgttgt tttcgctcag ctatgagcag atgacgcaga tggcagaaga
     9061 ggaaataaag caatgcgatt ttcgcaggga tggcacccat tatgtctggg aagtgaacaa
     9121 ggctcatgac atcctccgct tctggtatct cctggcgctg cgtggacaca ctggcctggc
     9181 cacaacacgg gtcgaagcag actataaacg gcttaaaacg ctcatttccc agaggaatga
     9241 gggacaatga tgcgggaaaa agcccgacgt ccgtcgccgc tgcagcggcg ggtgctgatt
     9301 gtgctggctg cccttgatga aaaacggcca ggacccgtgc tgacgcggga catagaacgg
     9361 gggctggagc gaagcgggga agccccggtc tacgggccaa acctgcgcgc ctcctgccgt
     9421 cgtctggagg atgccggctg gctgcgtacc ctgcgcgctc cgaacctgca gctggcagtg
     9481 gagctgacgg acgctggccg ggccgtggca cagcctctgc tgctggcaga acaggaccgc
     9541 ctccgggcag aacagcgtgc ggcagaagtc gtcgtactgc cgctggtccc ggcagcaggt
     9601 ctgcccgcag acggcacttc tgccactgat ctggcggtgc agctgaacgg cataacgtat
     9661 caggcctgcc gtggggattt tgtggtgcgt ctcgacggca gcacctgtct gcagttatgg
     9721 aataaagaag gcagggtggt acgcctggag ggcgatccgc tggaagtggc gcagtggctg
     9781 caggcctgtc acgatgccgg aatggaggtt cgtgtacagg ttaatgaaag cgtcaccccg
     9841 taaaaacata ccagcacggt ttacacgtta tcccatggtg gcagccggtt aactttcctg
     9901 cgccaccagt ggaaagaggc catgagcagt ccgaaccaaa aaccccccgt gatacttata
     9961 ataatggcct cacctgccac cattcctgac ggcccccagt agataaacca cattgcacat
    10021 ccccaggaaa tgccccataa gccccccgtc agcaccgtga cctgccagaa cggcataaag
    10081 ggtaatggcg gaagccggat acccagccgc cagagaatac gcagaagagg aggggcataa
    10141 ttgctccgcc acatcttttt gctgtccatc agggcaatgg cccgggcttt tttctgctca
    10201 aaggtcacag gagcacctcc gttccatgat gtaaacgtga cacagtttat tgttattgat
    10261 tattattatc atgctgtgat tatgttgtca tgcaccggta cactgacaaa agaaagggag
    10321 aacaggaggc tggctgatga acaggaaata ttatcttaat ccgcgtaagc gggccgtgag
    10381 ggccgcttac aagcgtgtat gagatcagtt tggatattcg aaattattgt acgtttaacg
    10441 caccatcatt aatgctaatg tatgcataca ttagcattaa tgatgaagga aaagaataac
    10501 aatggcagtc aacccacttc ttttttggat ttcttaattt tcgtctgata aagccgcttt
    10561 acccgttcaa tttctgccag cgttgcggac cagtgtagtt cggcggcatg acgatatctg
    10621 gcagccattt tttccaccat ctccggattg ctgtccgcgc tgcggtgctg acgccccttc
    10681 tccccaaaaa cttcaacaac cagccactgg cttccggttc tgccgaacaa tgccttgcac
    10741 aaacgtctca cagtcagcgg ggttaaatct ggatcgggat cataggcaat ggtagcaaaa
    10801 ataaagcgca gcatttctga tgtcttgtaa cgctttacgc tggcagccag gcgtgcggca
    10861 cgttgcggtg cttcccagcg gtcgttactg taattagcca cttcagccag ataatccttt
    10921 tcaagttttt cagccgtgta gcacctgccg cctaaaaaaa gccgcatggc ctccctggtt
    10981 ttatttctca catatcatcc ctcattaatg ctaatgtacg catacattag cattaacatg
    11041 ctgacagtaa caagcggcgg atttacgtct ggataacgta aatgattgcg atatacaggc
    11101 caggcggtca gattttcagc cgtgcctgtt cctgcagcag ttccagatga tatgcagcaa
    11161 tagcgtttac cacccctgcc gccatccgtg cgtcggcgat cgctgagtgt gccagccctt
    11221 cccaggtcag tccggcctgg ctggcagcac tggccagtga aatcgtgcca tagcggttgg
    11281 tggccccata atatcctgca gccagctcca tcgcacaata taccgtcagt tcttccagcc
    11341 agtcagccgg atcgctatgt gcggccgcag tctgtttcag aatgcggatg tcgaaccggg
    11401 cattaaagat aattactggt cgatctccga ttgcatgccg cagttgccgc gccacatctg
    11461 tccatgatgg tgcgccgcaa agagcctgct cactgatacc atgcacggcg gcggcctgcg
    11521 ccccgatggc gaccgtgggc ttcagcctgg tttcaaatac cacctgaccg gccgcatcgg
    11581 tcaggccgat ttccagggct tccgcggtat tatccagtcc tgtcgtttca gtatccagaa
    11641 agagtggtgc cagactcagc cagtcatatg cctgccgtgc catccggcca ctggtgctgt
    11701 ttagtcggga taaaacggaa agccgctggc cagccagctg ttgtttactc gtcagagggc
    11761 gtttctcacg catcggaaca cagtctgcaa ttcggtatac agcgaacctg ccaccataag
    11821 tatttttata ccacttaacc ggctcagcac cgggggctgg cttcattctg aattcatcgc
    11881 gcagacgtcc tttcgagaaa cacctgtctt cggcaatcgc ctgcgcacgt ctggtgacca
    11941 gctcatcatt attcatggtt cttgcatctc cgtcagacgt ttttatatcc ggacagcccc
    12001 tctatggcca cttctgtgca gtatgcaata cacgcaggat atatactttt ccccactggc
    12061 tgtcgacctc ataaaccaga acaaaatgcg ggtgaataac cagttcccgg gtaccttcca
    12121 cacgaccgtt gcggccttta tacggctgcc cggccaggct gctggctgaa taaccaatcc
    12181 gttcgtccag ttcaatggcg gccatcaggt tctgctccgc aatgtactca cgaatgcgct
    12241 tcctgtcctg gctggccagc atggtccaga atatctccat catttatcgg caaccttcct
    12301 gctcatttca tcccgccatg cagcagcaga agattcgacc atgtcatggg gaagcacatc
    12361 gccgcgcttg agttgctgac gtcctgccgc aacctggtca cggaaccagg tattatgctg
    12421 ccgttcaacg gtctggcgca taaaatcccg gattaattga gagccattac ggtccatgct
    12481 ttttgcagcg gccataaaag catccttcag ttcagcgtcg attcttaaac tcatattaac
    12541 ctgtgccatt tatcacctcg tcattactac gtatatacac agtatataac gattcagata
    12601 tcgtcaccag gatatgccgc gtcagcggca tggaaggcgg cactctgttg ttgcatatga
    12661 cacaggagta aaaccgccga agcccggcgt aagccggtac ggactgatag atttcacctt
    12721 acccatcccc agccctgcta agccataccc gctttctgcc atgacagagc ttctgtgcgc
    12781 ggtcggagtg gtcccgacga gggtttaccc gaagtcgggg cgtatctccg cgttagcggg
    12841 ccgtcagggc cgcttacgag cgtgtttt
//
LOCUS       DAZFRE010000122        12797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFRE010000122 DAZFRE010000000
VERSION     DAZFRE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.122"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            142..1206
                     /gene="ulaG"
                     /locus_tag="ACHKUI_004287"
     CDS             142..1206
                     /gene="ulaG"
                     /locus_tag="ACHKUI_004287"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6600363.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHKUI_004288"
     CDS             1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHKUI_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6600364.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHKUI_004289"
     CDS             complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHKUI_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6600365.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHKUI_004290"
     CDS             2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHKUI_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6600366.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHKUI_004291"
     CDS             3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHKUI_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6600367.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3867..5492)
                     /locus_tag="ACHKUI_004292"
     CDS             complement(3867..5492)
                     /locus_tag="ACHKUI_004292"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6600368.1"
                     /translation="MHWQTHIVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     APQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGVRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFLEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5576..6739)
                     /locus_tag="ACHKUI_004293"
     CDS             complement(5576..6739)
                     /locus_tag="ACHKUI_004293"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6600369.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGHNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6742..7380)
                     /locus_tag="ACHKUI_004294"
     CDS             complement(6742..7380)
                     /locus_tag="ACHKUI_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6600370.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFTSR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7390..7788)
                     /locus_tag="ACHKUI_004295"
     CDS             complement(7390..7788)
                     /locus_tag="ACHKUI_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6600371.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7806..8465)
                     /locus_tag="ACHKUI_004296"
     CDS             complement(7806..8465)
                     /locus_tag="ACHKUI_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6600372.1"
                     /translation="MSGFFQRLFGKYNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8516..9214)
                     /locus_tag="ACHKUI_004297"
     CDS             complement(8516..9214)
                     /locus_tag="ACHKUI_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO6600373.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITTEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTATESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9233..9634)
                     /locus_tag="ACHKUI_004298"
     CDS             complement(9233..9634)
                     /locus_tag="ACHKUI_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO6600374.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9824..11419)
                     /locus_tag="ACHKUI_004299"
     CDS             complement(9824..11419)
                     /locus_tag="ACHKUI_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFO6600375.1"
                     /translation="MQKILRIDSNDNLIVALKDLHAGESFSWDDDNITLVTDVKAKHK
                     FATQDIPLDGIVSMYGTPVGKATRPIVKGEAITVDNIRHYAAPVTLEDVEPYHWQAPD
                     VSEWSTRTFKGYVRDDGRVGTASYWLVFPLVFCENRNVSKLTNALNDALGYTNNSLKK
                     FALNLTSGSDELIETARMFPHIEGVRCITVTSGCGGATSDCETMCDVLAAYADHPNVI
                     GMTVFSLGCEKAQQKMFKDALARRNPEFDKPALYFLQQEWDSEERMMQTALQQTFEAM
                     KAVKPTERVEVPLSCLKVGMKCGGSDGFSGISGNPAMGLVSDWLTTLGGASGLAEFPE
                     LCGAEGDMVKRCINLEDKKKFLNLMQGYEKTANFFDTTIADNPSFGNIADGLITDAIK
                     STGAAKKGGRAPIVSVCDYAEPMPDSGLSLVCTPGNDVDAVTGLVAAGCNVVIFSTGL
                     GTPTGNPIVPVLKISTNSTIARRLSDMIDFDCGPVIDGTPLEDVSRELFEKVIETASK
                     DYVVKADKLEQFDFMLWKRSLDL"
     gene            complement(11453..12754)
                     /locus_tag="ACHKUI_004300"
     CDS             complement(11453..12754)
                     /locus_tag="ACHKUI_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600376.1"
                     /translation="MANITQYDNPLLNSAIQKSWKRLVPLMFIIYFVAFIDRVNVGFA
                     KDAMKLDIGLSESAFALGAGIFFAAYALFGIPANLILNKIGAQKWLSITTAIWGLLSA
                     MTGFVTSETQFIILRFLLGLGEAGFYPGILLLASIYFPNKVRGSVVGIFVLGVPLALT
                     LGSPISGALLELHGWMGRPGWFWMFVIEGLPALVLGIFAWFYLDDNPTKARFLTQEEK
                     DALNKQLESERQKTETSSVISALKNAKVWHLALIYGTIQISVYGLMFFLPSQVASLMG
                     ESLGFKQSLVAAIPWACSAFGVYYIPRIADRNPSRRVAISVMCMLAAAVGLALSAFAG
                     PVVAIGALCLSAVGFLSVQPIFWTFPPQLLTGPALAAGIGFCTTMGAFCSFLAPLIRV
                     EVDRAMDSSIAGLMALSVITIGCALLIGLLQKSQQAAKAYS"
BASE COUNT         3435 a         3329 c         2922 g         3111 t
ORIGIN      
        1 ctttccacac tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc
       61 cttcctgaaa aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattaaa
      121 ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta tcacccgtga atcctggatc
      181 ctgagcactt tcccggagtg gggtagctgg ttgaatgaag aaattgaaca agaacaggtc
      241 gctcctggca catttgcgat gtggtggctt ggctgcaccg ggatctggtt aaaatcggaa
      301 ggtggcacca acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac
      361 ccgttaatga aacagggtca ccaaatgcag cgcatggctg gcgtgaaaaa actgcagcca
      421 aacctgcgca ccaccccgtt tgttcttgat ccgtttgcga ttcgccaggt cgacgcggta
      481 ctggcgactc acgatcacaa cgatcatatc gacgtcaacg tcgctgctgc tgtgatgcag
      541 aactgtgctg atgacgtacc gttcattgga ccgaaaacct gtgtggattt gtggattggc
      601 tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtgaaa
      661 gacattgaaa ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc
      721 gatcaaaaag cggctggcgt actgccggac ggcatggacg atcgcgcggt gaactatctg
      781 ttcaaaacgc ctggcggctc cctgtatcac agcggcgact cccactactc taactattat
      841 gcgaagcacg gtaacgaaca tcagatcgac gtggctttag gttcatacgg cgaaaacccg
      901 cgcggtatca ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat
      961 gcgaaagtgg ttatcccgtt ccaccacgat atctggtcaa acttccaggc cgatccgcaa
     1021 gagatccgcg tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt
     1081 atctggcagg ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac
     1141 tatccgcgcg gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc
     1201 ctgtaatctg tcgtttatac cggatagcgt ggagtagttc gcgctatccg gcgtagtatt
     1261 tcctggctat ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg
     1321 aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg
     1381 agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac
     1441 ttgacgaaag cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccaacagc
     1501 gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta
     1561 tcgctaaagc ggcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct
     1621 ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt
     1681 atctaccgct ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggcg
     1741 gacagtacaa caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct
     1801 atgccgggca ctggatgttt accagcggca aaggactgac agcagaaggg ttgtataaaa
     1861 ccgatatgct gacggcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg
     1921 tactggttga tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc
     1981 aaattgatat gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag
     2041 cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc actgcagcat
     2101 ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt
     2161 tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag tgattcatcg gcaggcacaa
     2221 cgtcatcgtc gaggccatgc cacagcagta gaggtctgtc accaagttgt tccaggtggt
     2281 ttgtcgcttc ccactctgcc agcggcgcga caatgttatt gaattcattc tgttgtgctg
     2341 ccgtttcagg tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcctgatc
     2401 ccatcatact ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca
     2461 tcgcgcccat cgacgcgcca ccgactgcca gacggtcatc acgcagccag ttttcttcgg
     2521 ctattgccgc acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt
     2581 gatttaaccg ccgcgcttcg tcgccactaa aacggctacc gtgatcgggc gcatccggca
     2641 tgatcacccg caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg
     2701 atgaagtaaa gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag
     2761 gataagcatg aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc
     2821 tctccttttt gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat
     2881 tagttcataa tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt
     2941 accaggtaac aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg
     3001 ttagcaggaa acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt
     3061 taagtgcctg tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc
     3121 cgcaagagat tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga
     3181 tggttcgggg ttctccggat gacgcattag cagaaatcaa agcgaaagct gtcgctgcaa
     3241 aagctgatta ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt
     3301 cacaagccat tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct
     3361 ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta
     3421 ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa
     3481 caattacttg cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag
     3541 tctgcagaat tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca
     3601 gttagcaata tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat
     3661 gaacaaggtg cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac
     3721 tggcgcgtac aggcaatact gtatgcatga gtgtcatcta cagcaaatga acaatgccgg
     3781 atgcggcgcg agcgccttgt ccggcctaca attttcacat atttcattga gttaatcgaa
     3841 accagcgtcg catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat
     3901 aaatcattct ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga
     3961 caccacgcct gcgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc
     4021 aggaatagct gatgagtaat ttctcgcccc agttcttcag ctggcttacg cagctgctgc
     4081 tgtaaacgac gaaccgcgcg atcaaaataa cgatcctgcc ctttcacttc caaaaatgct
     4141 tccgacaata agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac
     4201 ataatattgc cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc
     4261 agctcgctct cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac
     4321 ggcatacctc gtttgcagat cacaaatttt gccgcaggtg taaataaacg cgcccacaga
     4381 gcttctttgg catcggcgcg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt
     4441 tgcccttcaa gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc
     4501 aatggattac caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg
     4561 cgcatcatgg catggctacc tagggcgcaa tcaaaacgcg tcatcccccc cattttcagg
     4621 atcagacgaa ctccttcccc ttcctgcccc aacaaccagc caatggcatc ctgaaactcc
     4681 acttcgcagc tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc
     4741 gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta
     4801 gtctgcgcca gcaccagatg cgcatcgctt tgcggcgccg agaaaaacca tttatgcccc
     4861 accagccgat aagagccatc ttccaggcgc tctgcgcggg tggtgttgct catgacatcg
     4921 gaaccgccct gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct
     4981 ggcaataagt gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc
     5041 gtgggtaaca tctgtaacaa caatggcgtg gcggcaaagg tcatggttat cggacataac
     5101 gacccagcct caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg
     5161 gagcgagcgt cttcttccca ggcaagattg tgcacccgat tggtacatag tgcctgcatc
     5221 agcaggtgcc aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag
     5281 cgcaataatt caggcggatt cacattcgcc agccgcccca gttcaaggga ttcagccgtt
     5341 cctaactgct gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt
     5401 accgcttcgc agagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt
     5461 tgattaaaaa cgatgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac
     5521 cattagtatg gtcactgcca tggattcatg acagaaagag attgctaaaa cattcttatc
     5581 cagcaatgta atgcggaacg aaacgtgagg tgtctttggt gatcagcgtg ttatcttcac
     5641 ggatccccat tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat
     5701 cgccaaatcg cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac
     5761 catcagcgtg gtcaacgacg ttattgtgac cgtcgaaaat ggtgacattt ccgccttcgc
     5821 gcgagtagat cggtttacgc acatagcttt cgccagcggc aatctgcggt ttttcaccct
     5881 caaaccagga tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta
     5941 gccctttatt actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc
     6001 gcagcagcgg accattatcg tcacgcatca tccactccag cggatacagc ttaaatgcac
     6061 gctggatgac attatcatca agatcggtca gtacgccgcc gacgcccaaa ccgagatctt
     6121 caatgtagat aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca
     6181 cggtactcct gtcttcatcg gtgtcctgac agcagcaaaa ataaaacggt tcccgactgt
     6241 aaagctcgct aaagcgtgaa atcaggcgtt cctgaatagc attgtactga tcggcatcac
     6301 gcggaataat gccgctgcgc cgggcatcct ccagccacag ccactggaaa taagccgact
     6361 cgtacaatga agttggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac
     6421 accaggcaaa atccatccgt ccatacagcg aaggatcgcg tgcgcgccag ctttcagcga
     6481 tcacatccca gtacaacggc gggatcgcca gttgcgtcag gatctcttca tcttttaccg
     6541 cgcgatccac cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct
     6601 cttcaatctg gcgcagagta aaacggtaag cccgactctc atcccaatag atttcattgt
     6661 cgatgatatg aaagtcgaaa ccgttgttag cggcgatctg gtccagatcc cgtcgcacag
     6721 gaacgttgtg tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc
     6781 atagccgccg cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga
     6841 ctcttttttg ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg
     6901 gcgggaagta aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg
     6961 aataacccgg cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc
     7021 gtagtagcaa ttttcgtact tagactgaca attctgctga gtcatattct tcggaacatc
     7081 ggcataaaat gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc
     7141 gtcatcaata caatcctgca ctgtcgcgta aaacgttccg tcgccgtcgt tatcgacatc
     7201 gctactatcg ctacaacctt tcaatacgaa aaaagcggca ccgcccatta gcgccaacgt
     7261 taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat
     7321 gcgactaatc gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat
     7381 gatcgtccat taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt
     7441 tcccataaac attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat
     7501 ataaagtctg acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc
     7561 gaaataatct ggaatactca ccgcattgat tgccgcactg gataagggga taacgtaacc
     7621 caacaatgta ccgctgaacg ccagtgacgc cgcggtatta ttgtttttga ttaactgcca
     7681 ttcgttgtgc ggtgtaattt tggagtagat aaacagaaaa ataatcacca tcgccacgcc
     7741 aataaaaaaa taggcactaa aggcaagaag tgaatccagt atatgcattg taaacgtcct
     7801 tgtaattaac caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa
     7861 aataaccact ctggctcacc gagatcgtta aaagattcct caccatttag cagcaaatat
     7921 tcgtaagtat cttcggtcac ttgccgctgg tagcccatgg taaaattatg gacttcccat
     7981 ttagcatgat tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt
     8041 tcttcgctgt taaaaaatcg ctgccagcgt ttttcctgcc agtttaaggt cattgccccc
     8101 atcgttttgg cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct
     8161 tcatagacaa agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg
     8221 atttgcagaa actcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga
     8281 tcgatgtggc tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca
     8341 tcttccagca aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc
     8401 agcggaccgc gagcgatagc aggcttatta tacttgccaa acagacgctg gaaaaaacca
     8461 gacatatttt atccttaaaa tagttgccag ccttgcagtg aggctggcaa aaaaattact
     8521 cgccctgttg gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc
     8581 caattccggc ttccgccagc ttttcgtcaa ggtcgcgacc gtctgcgact ttctccaact
     8641 gtgcggcagc atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg
     8701 attctgtcgc tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt
     8761 gtgcacgctg catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga
     8821 tacgctgcgc tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg
     8881 acaacacttg ctcttcagtg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt
     8941 cgttaatcaa cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac
     9001 tggctttgcg ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca
     9061 gatcaacgcg agattttccg gctttatcga gttcagcttt agcgtcacgg atatgctgtt
     9121 ccagcatgcg cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt
     9181 tccccagcgt aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg
     9241 aatattcttc tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa
     9301 gcaggatatc ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt
     9361 cctgttgtac gctggagatc cctaccgatg acaacggcat catcttctga tttcttaata
     9421 agaaggtatt aaattcatct ggattgctga tactactcac cggacaaata aaggtttcga
     9481 taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt
     9541 taataattaa tgcgctctcg ctatttgtca catcaatatt ttgttcaggt acagtttgca
     9601 gcgccgtcgc cagcgccaac ggattccatg tcatatatta ttccatatag atttagttta
     9661 aatattaaca aaatgaatat ttgcagtaca taattatctt accagctata gacaaaaaaa
     9721 aaccatccaa atctggatgg ctattaagaa gaaccaaaaa tttaacgcat gcatacgata
     9781 aaaaatgcgc cagtctacat acactgacgc taatgattgg tcactacaag tctaacgacc
     9841 gcttccacaa cataaaatca aactgttcga gtttgtctgc cttcacgacg tagtccttac
     9901 tggcagtttc aataactttt tcaaataatt ctctactcac atcttccagc ggagtgccgt
     9961 caatcactgg gccacagtcg aaatcaatca tgtcagacaa tcgtctggca atcgtgctgt
    10021 tggtggatat ttttaacacc ggaacaatcg ggttaccggt gggcgtacca agacccgtcg
    10081 agaagataac cacattacat cctgctgcta ccaatccggt taccgcatcc acgtcattgc
    10141 ccggagtaca caccaacgat aatccactat cgggcatagg ttcagcgtaa tcgcatacac
    10201 tcacaattgg cgctcgcccg ccttttttcg ctgctccggt agattttatt gcgtcagtaa
    10261 tcaacccatc cgcgatgttg ccaaaacttg ggttatccgc aatggtggta tcgaaaaagt
    10321 tcgctgtctt ttcgtagcct tgcattagat tgaggaactt ttttttatcc tcaagattaa
    10381 tacaacgctt aaccatatca ccttctgccc cacataattc tggaaattct gctaacccgg
    10441 aagcgccacc aagcgttgtc aaccagtcag aaaccagccc cattgcaggg ttgccggaga
    10501 taccagaaaa accatcggaa ccaccacact tcatccccac tttgagacag gaaagaggga
    10561 cctctactct ttctgtcggc ttcaccgctt tcattgcctc gaaagtctgc tgtaaagcgg
    10621 tctgcatcat ccgctcttcg ctgtcccact cctgttggag aaagtagagc gcaggtttat
    10681 caaattcagg attacgtctc gccagcgcat ccttaaacat tttctgctgc gctttttcac
    10741 atcctaaact gaataccgtc ataccgatta cattcggatg gtcagcataa gcagctaata
    10801 catcgcacat agtttcacag tctgaagttg caccaccaca accactggtg accgtaatac
    10861 agcgaacacc ttcaatatgc ggaaacattc tcgcggtttc gatcagttca tcgctaccgg
    10921 acgtcagatt aagagcaaac tttttcagtg agttattggt ataacccaaa gcatcattca
    10981 aagcattggt taacttactg acattacggt tctcgcaaaa taccaatgga aagactaacc
    11041 agtaactggc agtacccact ctgccatcgt cacggacata acctttaaaa gtgcgggtgc
    11101 tccattcaga gacatccggt gcctgccagt ggtagggttc aacatcctcc agtgtgacag
    11161 gagctgcata gtggcgaata ttatcaacgg taatcgcttc tcccttaacg atgggacgcg
    11221 tggccttgcc aactggcgta ccgtacatcg aaacaatgcc atccagagga atatcttgcg
    11281 tcgcaaactt atgtttagcc ttaacatcag ttactaacgt tatgttgtcg tcatcccacg
    11341 agaacgattc tccagcgtgc aggtctttca acgcgacaat gagattatcg ttactgtcga
    11401 tacgtaatat tttttgcata atcatattct tccagtcaga tgttcaatct tattaactgt
    11461 atgcttttgc tgcctgttgg ctcttttgca gcaatccaat caataatgcg cagccgatag
    11521 taattacaga caacgccatc aatccggcga tgctgctatc cattgcccga tcaacctcaa
    11581 cacggataag aggagcaagg aacgagcaga aagcgcccat cgtggtgcaa aaaccgatcc
    11641 ccgcagccag tgccggacca gtaagaagtt gtggagggaa agtccagaat attggctgta
    11701 ctgagaggaa accaaccgca cttaaacaaa gtgcaccaat cgccacaaca ggccccgcaa
    11761 atgcggaaag ggcaaggccg acagcagcag ccaacataca cataaccgaa atggccacac
    11821 gccgggaagg attgcggtca gcaattcgcg gaatgtaata gacaccaaaa gcagagcacg
    11881 cccatggtat tgccgcgact aatgactgct taaagccaag gctttctccc atcagcgatg
    11941 caacctgtga cggaaggaaa aacattaaac catatactga tatctggatg gtgccgtaga
    12001 ttaatgccag atgccaaact ttggcatttt tcaatgctga aatcactgaa ctggtttctg
    12061 ttttctgtcg ttcagattcc agttgcttat tcaaagcgtc tttttcttcc tgggtaagaa
    12121 atcgcgcttt cgtcggatta tcatcaaggt agaaccaggc aaagatacca agcactaatg
    12181 ccggcaatcc ctcaatgaca aacatccaga accatcctgg acgtcccatc cagccatgta
    12241 gttcaagtaa agctcccgat attggcgaac cgagcgttaa cgccagcggc acccccaaca
    12301 caaatattcc cacgaccgac ccgcgaacct tgttaggaaa ataaatggat gcgagcagta
    12361 aaattccggg atagaacccc gcctcgccca gccctaacag aaagcgcaaa ataataaatt
    12421 gtgtttcact ggtgacaaat cccgtcattg ccgagagaag gccccaaatt gcagttgtaa
    12481 tacttaacca cttttgtgcg ccgattttat tgaggatcag gttagctgga atgccgaaaa
    12541 gtgcataggc agcgaaaaat attcctgcac cgagagcgaa tgccgactct gataatccaa
    12601 tatccagttt cattgcatct ttggcaaagc caacatttac ccgatcaata aaggcaacaa
    12661 aatatattat gaacattaag ggtactaaac gtttccacga tttttgaatt gcggaattca
    12721 acagcggatt atcgtattgg gttatattag ccataaataa atccttagtt ctgttggtaa
    12781 gatattctat ttatagg
//
LOCUS       DAZFRE010000123        12687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFRE010000123 DAZFRE010000000
VERSION     DAZFRE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.123"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            64..1287
                     /locus_tag="ACHKUI_004301"
     CDS             64..1287
                     /locus_tag="ACHKUI_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6600377.1"
                     /translation="MGTIQKIRTDVNVLHAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1272..1916
                     /locus_tag="ACHKUI_004302"
     CDS             1272..1916
                     /locus_tag="ACHKUI_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6600378.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
     gene            complement(2287..4434)
                     /locus_tag="ACHKUI_004303"
     CDS             complement(2287..4434)
                     /locus_tag="ACHKUI_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6600379.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
     gene            5615..5803
                     /locus_tag="ACHKUI_004304"
                     /pseudo
     CDS             5615..5803
                     /locus_tag="ACHKUI_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            5782..5955
                     /locus_tag="ACHKUI_004305"
                     /pseudo
     CDS             5782..5955
                     /locus_tag="ACHKUI_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            6107..6652
                     /gene="cobU"
                     /locus_tag="ACHKUI_004306"
     CDS             6107..6652
                     /gene="cobU"
                     /locus_tag="ACHKUI_004306"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6600380.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6649..7392
                     /gene="cobS"
                     /locus_tag="ACHKUI_004307"
     CDS             6649..7392
                     /gene="cobS"
                     /locus_tag="ACHKUI_004307"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6600381.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            7404..8483
                     /gene="cobT"
                     /locus_tag="ACHKUI_004308"
     CDS             7404..8483
                     /gene="cobT"
                     /locus_tag="ACHKUI_004308"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6600382.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNSIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            8545..9480
                     /gene="ldtA"
                     /locus_tag="ACHKUI_004309"
     CDS             8545..9480
                     /gene="ldtA"
                     /locus_tag="ACHKUI_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6600383.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(10287..10556)
                     /locus_tag="ACHKUI_004310"
     CDS             complement(10287..10556)
                     /locus_tag="ACHKUI_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6600384.1"
                     /translation="MPTAPLFGRDLKKCMELKWVYLHCCYDNACVESFFHSLKVECLH
                     GEHFISREIMRATVFNYIECDYSRWRRHSWCGGLTPEQFENQNLA"
     gene            complement(10505..11355)
                     /locus_tag="ACHKUI_004311"
                     /pseudo
     CDS             complement(10505..11355)
                     /locus_tag="ACHKUI_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11501..12235)
                     /gene="clbA"
                     /locus_tag="ACHKUI_004312"
     CDS             complement(11501..12235)
                     /gene="clbA"
                     /locus_tag="ACHKUI_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis phosphopantetheinyl
                     transferase ClbA"
                     /protein_id="HFO6600385.1"
                     /translation="MRIDILIGHTSFFHQTSRDNFLHYLNEEEIKRYDQFHFVSDKEL
                     YILSRILLKTALKRYQPDVSLQSWQFSTCKYGKPFIVFPQLAKKIFFNLSHTIDTVAV
                     AISSHCELGVDIEQIRDLDNSYLNISQHFFTPQEATNIVSLPRYEGQLLFWKMWTLKE
                     AYIKYRGKGLSLGLDCIEFHLTNKKLTSKYRGSPVYFSQWKICNSFLALASPLITPKI
                     TIELFPMQSQLYHHDYQLIHSSNGQN"
     gene            complement(12236..12448)
                     /gene="clbR"
                     /locus_tag="ACHKUI_004313"
     CDS             complement(12236..12448)
                     /gene="clbR"
                     /locus_tag="ACHKUI_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis LuxR family
                     transcriptional regulator ClbR"
                     /protein_id="HFO6600386.1"
                     /translation="MDKFKEKNPLSLRERQVLRMLAQGDEYSQISHNLNISINTVKFH
                     VKNIKHKIQARNTNHAIHIANRNEII"
BASE COUNT         3321 a         2657 c         3107 g         3602 t
ORIGIN      
        1 atgagaaggt ttaagtttgt agttcagaat attactgcca agatagttta tactaggata
       61 tgtatgggaa ccatacagaa gataagaaca gatgttaatg tattacatgc ggccattcac
      121 cgtattgagt ggttgtttga gacattttca tctgtttgtt tgtctttttc tggaggaaaa
      181 gactctactg tgctgctcca tcttacggcc gatgtggctc gcaggaagaa acgtcgtttc
      241 tctgtattat tcattgactg ggaagctcag taccagtgca caattgcaca cattctgaag
      301 atgagggaga tgtatcggga tgtgacggag actttttact gggtggcact ccccctgact
      361 acggtaaacg gtgtctctca gtttcagccg gaatggatat gctgggaacc aggtgtggag
      421 tgggttcgtc agccaccaga tgacgctatt acagatatgt cgtatttccc attttatcgg
      481 tatgccatga cgtttgaaga atttgttccg gcattttctt cctggtttgc cggtaaccgg
      541 tgtggagtgg caatactgac tggtgttcgt gctgatgaat cgctcaatcg ctttatggga
      601 ctggtgtctc agcgcaaact gagatatgca gatgataaac cctggaccac agcgtcacct
      661 gaagggtttt attacacctt gtatccgttg tatgactgga aagcccgtga tatatggata
      721 tataacgcca gaacccgagc tatctacaat cccctgtatg acctgatgta ccgtgccggc
      781 gtgccgttac gcaacatgcg ggtctgtgag ccttttggcc cggaacagcg taagggactg
      841 tggctttacc atgttctgga gccggaaact tgggccagga tgtgtgagcg ggtgtcgggt
      901 gctgccagcg gggcgcttta tgccaatgag agcggtgcct attttgccct gcgtaagcgt
      961 atcacgaagc cgcctcatca tacctggcgt agctatgcga tgttcctgct ggatgtgatg
     1021 ccggaaagaa cggcagaaca ttaccgtaat aaaattgctg tctacctgcg ctggtatcag
     1081 acgcggggct tcccggatga catcccggat gaacaggaga atgacctggg gagccgggat
     1141 atcccgtctt ggcgacgtat ctgtaagaca ctcataaaga atgatttctg gtgccggacc
     1201 ctctccttca gtccgaacaa accccggcac tatgaacgtt atctgcagcg tatgaaagaa
     1261 aggaggaagg aatgggggat tctgtgacac ctgaggtgga agtattgagc aacatgattc
     1321 gacagtactt cagtcaggaa cgatctgaag aagagaccat acgagccctt aatcatctgc
     1381 gcagggtgtt gcatgaggtc agcccttttg cacaggagcc tgtggactgt gtgttgtggg
     1441 tgaaagcgga tgaggtggtg gccaacgatt acaatcccaa tgtcatgtct tccagtgaaa
     1501 aaaaactgct taaacattca ctggagcagg atggattcac gcagcctgtg gtggtttcgg
     1561 aagagaagga gcattatctg gtcgtggatg gttttcatcg tcagttgctg ggcaggaaag
     1621 cggatacgag aaaacgtctg aagggctggc tgccagtgac ctgtattaat ccggaaagaa
     1681 agggacaggc atcacgtatt gccgcaacca tacggcataa ccgtgccagg ggaaaacacc
     1741 agataacgtc aatgtctgac attgtcaggg acctttcgcg tctgggctgg acagatgagc
     1801 gtattggcac agagttgggc atggaccagg atgaagtgct gaggctgaaa cagattagcg
     1861 ggctgacaga gctgtttcag gaagagaatt tcagcccttc atggacggtt cgttgatatt
     1921 gtaaaatatg atagttggag taaggggcaa tagaaggaga atatttatcc caccttcttc
     1981 cgagtcggga taaatcaaaa ggtattatcc attgattcag tatctgggtg agagtaaata
     2041 atctaaaaat agtattgcgt tgtgaactga tatcactttt gaattacaaa ggtataggct
     2101 cagtaaccag ctttataagt cattgtcgta gcaatgacta tagagtcatg ctggcgggaa
     2161 gaataaatat tcacccccag atggaaaaca acgtcgaaaa accttcaaca aacacaatac
     2221 gttcgttagc ctgcccgcaa agcgggcaga tcaggacaaa gaattccata acgattgttt
     2281 ttgtcatcag aaattccata caaaacctat agctgcatta aaacctgctg caggaatagt
     2341 ttcatgtgct gtacggtagc gtttgtctgt caggttattg agtgctaggt taatccggta
     2401 ctgatcctcg ttaccgaatt ctgtatttac tgcaaagttg agagtggccc agcccggaac
     2461 atttgtttcg gtaccgttac tgtcatcttt tgcactagag gcagcacgaa taaaaacatc
     2521 agagattatg ttggcctgac ccatcacaag agtatgtttc agccctatac gtccgtttat
     2581 cgctggttct cctgtattag ttgtttttaa tgttgaagtt tcatattgcc gacgaattaa
     2641 attgccactg atatatggcg agaaaaccca gccattatat tccgcgctta tttccagtcc
     2701 ccatgttttt gcccgatcaa tattgtcata ataatagtag ctactacggg aggagttggt
     2761 gttaccattg caaactatac tgccatcaca gatcagactt gcaatataat ctttagcttc
     2821 tgagtagtaa actgcgctgt caatcagcca ctgattacca ttatatcgtg cacctaattc
     2881 aaagttattg gagtgttcag ccttaagatc aggatttccg tatgtgacac tgccgcccgc
     2941 agatgtctgc ataaaaagct gggagagtgt gggaaatacg tagccttgcg cgaacgcagc
     3001 gcgtaactcc aagttatcga aacctgaata gcgcaggctt gtagatgtta ccatttcgtg
     3061 atcactcgca gactctctgg caagagaggt atcgcttata atgcctgcgg tatatgatac
     3121 tccgtcacca cgcgtcaact ttgaagaaag ccagtattgg cgaactccca ttgtccatgt
     3181 ccagtgatcg gcgaatcgcc agtcattttg tgcgaatagt gagactgtac tttgctctga
     3241 ctcataatag gagcgagttc gtgtttcctt attaatgaag ccggtcagag atttgcttga
     3301 ggtcatgcca ccggaccttt ggctgaccct gtcttgtttg tactgtgcac cggtaacaag
     3361 ttcattatta gcaggcagcg aaaagtgact ctgcaatgtg accgcctgag tgtattgctt
     3421 atcatgggtg tcagtcttgt tatgaacggt cagagcctga atcatcggac tgggaacagg
     3481 ctgtgtcgtt tttacttcgt tggcaaattg gcgctggatg gtctgctcat acgcgtcgaa
     3541 atgaattttt tttagatagt caccgtccac gtctgtgtca tagaataccc caactttctc
     3601 tcgttcaagt ttaggtattt tgacactaaa tgcctcatag cttccgtctg gatcctcata
     3661 gtaagtttgc gtcgcgagtc tgtagcgatc aagcgagagg ccaaaacgat ggtttccgga
     3721 gttataaccc aaccatacac cctgactatt gttacgatag ttggtattcg gcagacgtcc
     3781 atccggcgta tcacgattgc cctgatcaga ataactaccg ttgatgcgat aatcaaatcc
     3841 accgatgctc ccctggaccg cgattgattc ttcccagcct gctgttgcgg aattataaac
     3901 agctttcaca actccagatg caagtttgtc acctcccttt ttggttatga agttaacaat
     3961 accgccaatt gcctgtgaac cgtacagtac ggaatatgga cctttcacta cctcaacacg
     4021 ctccagcgca gactcatcta tcaacagtcc cacaccataa ttatctccgg cgcgctgata
     4081 agttacctcc tgaccatcaa tgagaattaa aacacgggag gatgcttcgc cacgaatgcg
     4141 gatttgttta cggcctgcca aggagttgtc tgttatctct actccgggga tgtcctgcaa
     4201 attatcggct atggaggcat tggtggagtt ctgcaatgtt tgttggtcaa taacctgtat
     4261 agtcgccggg ctttcccaaa ggttgcttgc cgaacgattc gtggtcacga cgagcgtctc
     4321 ttcgttgctg atttttgttt ctgaagaagt tgcggttgca gtagaaacta tcccagaaat
     4381 gcataatgac agagtggaaa ggatataaat tcgacaaggt atattatata acatttcatc
     4441 cctcattaat acaaagtaca aatgattgct gtaatgataa ttattatcat ttatgttctc
     4501 atgtatctgt gattattgtc atcttcataa attgtaaata tcaaacattt ttgaccttaa
     4561 tcatgttttt tgatagatat tttacgactg aacatatccg tcgtttcctc tgactcgtgt
     4621 cagtcggtct ctggttgtat ccacggaccg cactcttgac ataagagtgc ggtccgtgga
     4681 aaacggagtt gaaaaaatgg tgtctgaaag aaggtaccat cattgcgatg acaaacactt
     4741 tggcgtggat aatgtaatta attgctgaaa ttgaccgtat atgcggtaag actcatatca
     4801 gtatcaggtc cggttaattg ctgatactga cataaacttt ttacaaaagg atgaatgcag
     4861 aaggttactc taatggccag agtgatgaca atgatggtca gacgttgacc tgcagaaact
     4921 gcattttaat tggtctataa agtctgcaag aggaatcagg gtcagatcag tgagtcttat
     4981 cgcggcccga agctatgact gaaagaaggc tggatacaga attgcaacaa tttggcaatg
     5041 tcagaacaca gaagctttgc aagcaggtga aaggttatgt ctagaaaaag gagtatatca
     5101 gaatacatct actgatgtac aaaacacaac aatagcttca tgatatataa atacgtgatg
     5161 acgagtggta ctccgcatgg ttatttaact tctcagcacc actaatgatc ctgagcaact
     5221 tcgtatttgg gccattgcca tggagtagaa agctgtgtct gagaatgcag aatttgagaa
     5281 aagaattcgc tttcttggag agcttctgaa aatggcacgc taacaacgct ttgttaaaaa
     5341 atgtgaatca cttgccgagg ctgcattttc tattcgagga gaatgttgac gtagatatta
     5401 cagaaatctc agcgcatcag gataaactgc taccttaggt caaccaccaa aagaaaatac
     5461 aatacgtcca gtttgtatgc ctttaataac acatctttta aatgttgaag aattatcccg
     5521 cctgctgaag aacgttgctc tgagtgtgat aaaccttttt attcttgatg atgtcagcga
     5581 gaagatggaa tatatcccac gactgggtcg tggttacccg gttaaacatc tggtggcgct
     5641 aataaatctg gcaagtcaca ttctgttgaa tgctgtgaca gtgccttacc gagagagcga
     5701 aattgtggtg ttctagtgca tagtcgccac cattccggat aattttattg cgttgtttta
     5761 caacttcttt tacagcgcag atctgtcgct gacgttgaat caatagcctg gatttcagaa
     5821 cacaatgccc gtttgctggt tggtccggca gtagcattaa ttcattatct gaatgaaatg
     5881 tgtcaatcca cagaaaccgc tgtcaatgac gcattttgtc ggacgttaac acgagaaagt
     5941 ctggctattc cgtaatgcca tcttatttcg taatggattg agaatgctgg atttttaatt
     6001 atattacgcg cagtattaat cgcgtaaatg ttttgcggtc agcaacggtg ggggatccat
     6061 tgctctgttc aatgaatacc atcatacatt tgaatcagga gtcggtatga tgattttggt
     6121 gacgggaggc gcacggagcg ggaagagtcg ccacgcagag gtgcttattg gggactcttc
     6181 acaggttctg tatatcgcta cctcgcaaat ccttgatgat gagatggctg cacggataga
     6241 acatcatcgg caaagccgcc cggagcactg gcgcacagtg gagcgctggc aacatcttga
     6301 tgaattaatt catgcagaca ttaacccgaa tgaggctgtg ttgctggaat gcgttaccac
     6361 aatggtgact aatctgttgt ttgattatgg cggcgataaa gaccctgatg aatgggatta
     6421 tcaggcgatg gaacaggcga ttaatgctga gattcagtcg ttgattgctg cctgccaacg
     6481 ttgccccgca aaggttgtat tagtgactaa cgaagtggga atggggattg tgccggagag
     6541 tcgtctggca cgacattttc gtgatattgc cgggcgggta aatcagcagt tggccgctgc
     6601 ggcaaatgaa gtatggctgg tggtttcggg tattggagta aaaatcaaat gagtaaatta
     6661 ttttgggcga tgctctcatt tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga
     6721 ctggatttcg agcattattc tcgcggtatt attacttttc ctttgattgg attattgctt
     6781 ggcgcgatta gcgggctggt cttcatggtg ctgcaggcat ggtgtggcgt accactggcg
     6841 gcactgttta gcgtacttgt gctggcgctg atgaccgggg gattccatct ggacggcctc
     6901 gctgatacct gtgatggcgt attttctgca cgtagccgcg atcgcatgtt ggaaatcatg
     6961 cgtgatagcc gtttaggcac ccacggcggt ctggcattga tttttgtggt actggcaaag
     7021 attctggtgt taagcgagtt ggctctgcgt ggcgaaccga tccttgcatc gctggcggca
     7081 gcatgtgcgg tcagccgcgg gactgccgca ttattgatgt atcgtcatcg ttatgcgcgg
     7141 gaagaagggc ttggcaatgt gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca
     7201 ctcggcttag ccgctatttt tgctgccgta ttattgccgg gtatgcatgg tgtggctgct
     7261 atggtggtga cgatggtggc gattttcatc ctcggtcagc ttctcaaacg tacgctgggc
     7321 gggcaaacgg gcgatacgct gggagcagcc atagagtttg gtgaactggt tttcctgctg
     7381 gcactgctat aaagagacgt gacatgcaaa tacttgccga tttactgaat acgatcccag
     7441 ccatcaattc tgccgctatg tcgcgtgcac aacggcatgt tgacgggtta ctcaaacctg
     7501 ttggtagcct gggaaagctg gaggcgcttg ccatacaact ggcaggaatg ccggggttga
     7561 atagcatacc gcatgtgggc aaaaaagcag tactggttat gtgtgccgat cacggcgtct
     7621 gggaggaagg ggtcgctatt tctccaaaag aagtgacagc catacaggct gaaaatatga
     7681 cccgtggaac aaccggcgtg tgtgtgctgg cagcacaagc gggcgctaac gtccacgtaa
     7741 ttgatgttgg tattgatact gctgagccta tccccgggct tatcaacatg cgtgtcgcac
     7801 gaggtagcgg caatattgct tcagctccgg caatgagtcg ccgtcaggct gaaaagttgc
     7861 ttttggacgt catatgttat acgcgggagc tggcaaaaaa cggtgtcacg ctgtttggtg
     7921 taggtgaact ggggatggca aacacgacac cggcagcggc aatagtcagc acaatcactg
     7981 gccgggatcc tgaagaagtg gttgggattg gcgcaaacct gccgacagat aaactggcta
     8041 ataaaattga tgttgtgcgt cgggcgatta cgttgaatca accaaatcct caggatggtg
     8101 ttgatgtcct ggcaaaagtg ggtggatttg atttggtcgg aatagctgga gtgatgttag
     8161 gtgctgcttc ctgcggttta cccgtgttgc tggatggatt tctttcttat gctgctgcgc
     8221 tcgcagcctg ccagatgtct cctgcaatca aaccgtatct cattccttct cacttgtcgg
     8281 cagaaaaagg cgcgcgtata gcgctctcgc atttggggct ggagccttat ctcaatatgg
     8341 atatgcgttt aggtgagggg agtggtgcag ctctggcgat gtccatcatc gaagctgctt
     8401 gtgcgatata caacaacatg ggcgaacttg ctgccagtaa tattgttcta ccggggaata
     8461 cgacttctga tttgaacagc taaaaacgaa aagtctgata aaagctatac ttatcggata
     8521 gtattcgcga cttaggaaag ggacatgatg cgtcgtgtaa atattctttg ctcatttgct
     8581 ctgctttttg ccagccaaaa tagcctggcg gtaacttatc cattacctcc agagggtagc
     8641 cgtttagtgg ggcagtcgct tactgtaact gttcctgatc acaatactca gccgctggag
     8701 acttttgccg cacaatacgg gcaagggtta agtaatatgc tggaagcgaa tccgggcgct
     8761 gatgtttttt tgccgaagcc tggctcacaa ctcaccattc cgcagcaact gattttgccc
     8821 gccactgttc gtaaagggat tgtggttaac gtcgctgaaa tgcgtcttta ttactatccg
     8881 ccagacagta atacagtgga agtctttcct attggtatcg gccaggctgg gcgagaaacc
     8941 ccgcgtaact gggtgactac cgttgaacgt aaacaagaag caccaacctg gacgccaacg
     9001 ccgaacaccc ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg
     9061 gggcccgata accccatggg gctgtacgcg atttatattg gcaagttgta tgccatccac
     9121 ggtaccaatg ccaattttgg tattgggctc cgtgtaagtc agggctgtat tcgtctgcgt
     9181 aatgacgata tcaaatatct gtttgataat gttcctgtag gcactcgtgt acaaattatt
     9241 gatcagccag tgaaatacac aacggaaccg gatggttcaa agtggctgga agttcatgag
     9301 ccgctgtcgc gcaatcgtgc agaatatgag tctgaccgaa aagtgccatt gccggtaacc
     9361 ccatctttgc gggcgtttat caacgggcaa gaagttgatg taaatcgcgc aaatgctgcg
     9421 ttgcaacatc gatcgggaat gcctgtgcaa attagttctg gttcaagaca gatgttttaa
     9481 gagcgttggt aattagaaag taaaaagcct gctcgaaagc aggctttttt gaatttggct
     9541 cctctgactg ggatcgcctt tgccagtaag tggctaataa gtaagctaaa ccagagttat
     9601 cttcctgtca agaccaccag aatgaccacc aataggagct ggctacataa atttggtttg
     9661 agcttcccta ctgcatgtgg tgatagttac tatagtgtcc ctacgtggga agcgctgatc
     9721 ctctccccta gtggaactgt gtctaaagag cgataacagt ccgctcaggg cgtgaagcgg
     9781 aagttgctaa atcagtctat gttaatcaac gaggagctgg tagataagat tgacactaaa
     9841 aattatggtg atggttccaa taacgtgttt gttatctgga ctttatcatt aaaatcctct
     9901 acaaaatccg agtgttttaa atttctaggc ttcgtacatc tacaatctgt actcgttgaa
     9961 atttcttcat ctccagagaa gtactacaag ttacgtatcc atcaagaacg tgttatggaa
    10021 ggtatgatgc ctgaagaacc aaaagccata gttatagaag atactgagtg ggggtgtatc
    10081 cactcgcagt atcactacca agataaagat cctgcgataa taacatgtat cgtaaacgta
    10141 aataatactg acttaatagc tacagagctt gtgaactcag tgttgaatac atcctgaatt
    10201 tcttctctcg atgctggtac acagcgatca tataaagtta ccgcgaaaga aacgactgat
    10261 cctaccctcg taatatggac actgctctaa gcgaggttct ggttttcaaa ttgttccgga
    10321 gtgagaccgc cacaccaact gtgccgtcgc cagcgactgt aatcacattc gatataatta
    10381 aacaccgttg cccgcattat ttcccggctg ataaagtgtt ctccatggag acattccact
    10441 ttcagtgaat gaaagaagct ttccacgcag gcattatcgt agcagcagtg taaatagacc
    10501 cattttagtt ccatacactt tttgagatcc cggccaaata atggcgctgt cggtattcct
    10561 ccggtgtcat attattcagc gattcatgcg ggcgttcaca gttatatttt gataaccatt
    10621 tttcagtgat ttcccgtact tcattcagcg ttctgaacag ataaaaatcg agtatttctg
    10681 ttcggtatgt ccggttaaag cgctcaatga aagcgttctg cgtcggctta cccggctgga
    10741 taaactccag ttttattgca tgtttctctg cccattcagc cagtgccagg gagataaatt
    10801 ccgggccgtt atccagacga agcatggccg gatagccacg gtttgccgcg atcctgtcga
    10861 gtacacggac cactcgctga gctggcagat tcagatcgat ttcaatcgac aatgcctcac
    10921 ggttaaaatc atcaacgaca ttgaacatgc gaaaacgacg gccacagacc agggcatcat
    10981 gcataaaatc gacagaccag ctcaggttca gactccggca ggcctgacgg atactgagtt
    11041 cgaacgtcgt tatcagatga gtaaccagct cacgcttaaa ggctggtttt aaagcttttt
    11101 ttcgataacg tctttcagcg cccggttctc cagactcagg tcggcaaaca tttgttttag
    11161 acggcggttc tcgtcctcaa gatccttgat tttcttaata tcagaagcct ccatgccgcc
    11221 gtatttggac ttccagttgt aataggtggc ttcagagatt ccggcctcgc gacagacatc
    11281 tttaacagtt cgtccggctt caaccgactt aatcacggcg atgatctgat gctcagtaaa
    11341 acgggcttta cgcatagcgc tctccttcgt tggcagattg attatgccgg atgatctcta
    11401 aatgtgaatg gcacgattat gcgggatact tacaccaccg acggaatatg aaaatcaata
    11461 ttatcgacgg ctcagaagtg tctagattat ccgtggcgat tcaattctgc ccatttgacg
    11521 aatgaattag ctgatagtcg tggtgataaa gttgggactg cataggaaat agctcaatag
    11581 ttattttagg ggtgatgagt ggagaggcta atgcgagaaa tgagttacat attttccatt
    11641 gagagaaata aacaggtgaa cctctatatt ttgaagttag ttttttattt gttaaatgaa
    11701 attcaataca atccagtcct aaagataggc ctttacctcg atatttgatg taagcttctt
    11761 tgagcgtcca cattttccaa aaaagtaatt gaccttcata acgaggaagt gaaactatgt
    11821 tagtagcttc ctgtggagta aaaaaatgct gactgatatt cagataagag ttgtctaaat
    11881 ctcttatttg ttcaatatcg acaccaagct cgcagtgaga actaatagca acggctactg
    11941 tatctatagt atgggaaagg ttaaaaaaaa tcttttttgc caactgagga aaaactataa
    12001 atggtttgcc atatttgcac gtactaaatt gccatgattg taatgagaca tcaggttgat
    12061 atctttttag tgctgttttg agcaggatac ggcttaaaat atagagttct ttatcactca
    12121 caaaatgaaa ctgatcatag cgttttattt cttcctcatt gagatagtga aggaagttat
    12181 ctctactggt ttgatgaaaa aaactagtat gtccaattaa tatatcaatc ctcatttaga
    12241 taatctcatt cctgttagca atgtgtatag cgtgattcgt attccgagct tgtattttat
    12301 gtttgatgtt tttcacatga aactttactg tgtttattga tatgttaaga ttatgtgata
    12361 tttgagagta ctcatcacct tgtgccagca tgcgcaatac ttgtctttca cgcagagata
    12421 acgggttttt ttctttgaac ttatccatgt ttccccccat cctgaatggt atctgtgtat
    12481 ctgtgtatct gtgtatctgt gtatctgtgt atctgtgtat ctgtgtatct gttgttttgg
    12541 cagtatttaa gaggaattta cgatacaacg gtttttatgt aaatgggaat tacgcattat
    12601 tttctatgtg gtggctgtat caattcatac ccgctacatc atgtatgtac tgcatgactt
    12661 acatgttttt gcttacagac agaatct
//
LOCUS       DAZFRE010000124        12645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFRE010000124 DAZFRE010000000
VERSION     DAZFRE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.124"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..2794
                     /locus_tag="ACHKUI_004314"
     CDS             <1..2794
                     /locus_tag="ACHKUI_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6600387.1"
                     /translation="WMVNGLAGDALHKTGKGTLVVAGSGENPGTLNTGDGTVILAQKA
                     DAAGRVRAFSEVRIVSGRPVVVLQDSHQIEGERIRWGYRGGTLDINGNDMTFHRLAAA
                     DEGAVLTSRAGSATVRLDFSPSGQKAVMWHGHFTGNLSVQNNTSSAVDFIMDGGADMS
                     GSFTQQGGGLYIQGHPVVHAVSSEAVAAALRKQGDNSVLTRPVSFTQKDWESRTFSIG
                     QLKLKEASFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAAGG
                     ASGTSTFRGNVNMRHSSLTVRDHFTGSITASDSRIVVNSENVRLEGDSRLTSSALTVS
                     DGGRLHVKGGLETDGGVTLDGGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDFM
                     TGAAGLLRGYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTAG
                     SMDISDSVLVMRTDLHHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPAG
                     TGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVVDEVSRPSLPV
                     VMRQDAKTPNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDETRWLLTG
                     YRSTVNASAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSA
                     GGGFSDNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYAS
                     ALFDSGAYIDLIGKYVHHDNEYTATFAGLGTKDYGSHSWYAGAEVGYRYHVTEDAWIE
                     PQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAG
                     LGYQFDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFG
                     KYNVDNAVNAGFRYSF"
     gene            complement(3360..6032)
                     /gene="rcsD"
                     /locus_tag="ACHKUI_004315"
     CDS             complement(3360..6032)
                     /gene="rcsD"
                     /locus_tag="ACHKUI_004315"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6600388.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            6771..7898
                     /gene="ompC"
                     /locus_tag="ACHKUI_004316"
     CDS             6771..7898
                     /gene="ompC"
                     /locus_tag="ACHKUI_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6600389.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVVDNVTHNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8010..9065
                     /gene="apbE"
                     /locus_tag="ACHKUI_004317"
     CDS             8010..9065
                     /gene="apbE"
                     /locus_tag="ACHKUI_004317"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6600390.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            9139..10203
                     /gene="ada"
                     /locus_tag="ACHKUI_004318"
     CDS             9139..10203
                     /gene="ada"
                     /locus_tag="ACHKUI_004318"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6600391.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10203..10853
                     /gene="alkB"
                     /locus_tag="ACHKUI_004319"
     CDS             10203..10853
                     /gene="alkB"
                     /locus_tag="ACHKUI_004319"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6600392.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLAGDCRYNLTFRQAGKKE"
     gene            10929..12572
                     /gene="yojI"
                     /locus_tag="ACHKUI_004320"
     CDS             10929..12572
                     /gene="yojI"
                     /locus_tag="ACHKUI_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6600393.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMVLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         3026 a         3022 c         3476 g         3121 t
ORIGIN      
        1 ctggatggtg aacggacttg cgggagacgc acttcataaa actggtaagg gaacactggt
       61 ggtggccggc agtggtgaaa atcctggcac cctgaatacg ggggacggaa cggttattct
      121 ggcacagaag gctgatgctg caggcagggt ccgggcattc agtgaagtcc gtattgtcag
      181 cggacggccg gttgtggtgc tgcaggacag tcatcagatt gagggggagc gcatccgctg
      241 gggataccgg ggaggcacgc ttgatatcaa cggcaatgat atgaccttcc accggctggc
      301 tgcggctgat gagggggcag tactcacaag ccgggcgggt tctgccacag ttcgtctgga
      361 tttcagcccg tcagggcaga aggccgtgat gtggcatggt cattttaccg gcaacctcag
      421 tgtccagaac aacacgtcat cagcggtgga tttcatcatg gatggcggag cggatatgtc
      481 cggcagtttt acacagcagg gcgggggact gtatatccag gggcatccgg ttgttcatgc
      541 cgtgagcagt gaggcggttg cggctgcatt gcgaaagcag ggtgataatt ctgttctgac
      601 gcggccggta tcatttaccc agaaggactg ggagagcagg acgttcagca tcgggcaact
      661 gaaactgaag gaagcgtcat tcagcctttc ccgcaatgcg acactgacgg gagatattga
      721 tgctgataat gccaccatgg tgctgggaag tgactcgctg tatctggata tgaaggacgg
      781 cacggggagc agcagtgcgc cggttaaggg gacgtctgct gcgggcggtg catccggtac
      841 cagtacattc cgcgggaatg tgaatatgcg tcattcttcc ctgacggtcc gcgaccattt
      901 taccggcagt ataacggcat cggacagccg gatagtcgtg aactcagaga atgtgcgtct
      961 ggaaggggat tcccgcctga cgtcttctgc acttactgtc agtgacggcg ggcgtttaca
     1021 tgtgaagggg ggactggaga cagatggtgg tgtgacactg gatggaggaa cccttctggt
     1081 tgacggggga agtgtcagga atgatgtgta tgaacgtctg ctggcctggt cagaggagcg
     1141 tggcgggctg aacggaagcg gtgagtatga ttttatgacg ggggcagctg gtctcctgcg
     1201 gggttatgtc agagggagtg caggtaatgt gaatctgcag aatgcggcct ggatgatgac
     1261 gggaaactca tccgtgaagc atcttgagtc atcgggaagt gctctgtatt tcagcaggcc
     1321 gggcggggag ttccataccc tgacggcagg cagtatggat atcagcgaca gtgttctggt
     1381 gatgcggacg gaccttcacc actcagacca gcttcgggtg acagaatcgc tgcggggtaa
     1441 gaataatctg ttgttggttg attttactga gcgttctgac gggcagaagg cgctgaatat
     1501 ccctctggtg acagcgccgg ccggtaccgg cgcggatgtg ttcagtgtga aaacgcgtga
     1561 taccggcttc agtcatatca cacctgttgt cagggctgaa caggggacgg gcggcacggc
     1621 ctggcagctg aatgttgtgc agccggaaac tgccgcagag cctgtggtgg atgaggtgag
     1681 caggccgtcg ttgccggtgg tgatgcggca ggatgcaaag acgccgaatc ctgtggaagc
     1741 cgtatcaccg ctcccttcac cggtttctgc tccgtcaccg gctccggagg cgtcagtgac
     1801 ggatgtactg accggagaga atgcggggga atcaggtgag taccgtgatg agacccggtg
     1861 gctgctgaca ggataccgga gtacggttaa tgcctctgcg gtcagggatg ccgggatgct
     1921 gatgtcaatg gggcaccgta acttcattaa tgaggtcaac aacctgaaca aacgtatggg
     1981 tgacctgcgg gacatcaacg gcgaggccgg tgcatgggcc cgtatcatga gcggaaccgg
     2041 ctctgccggt ggtggcttca gtgacaacta cacacacgtt caggtgggtg cggataaaaa
     2101 acacgaactg gacggtctgg acctcttcac cggggtgacc atgacatata ccgacagcaa
     2161 cgccggcagc gacaccttca gtggtaaaac gaagtcagtg ggtgcgggtc tgtatgcttc
     2221 agccctgttt gattccgggg cctatatcga cctgattggt aagtatgttc accatgataa
     2281 tgagtatacg gcgacctttg ccggactcgg cacaaaagac tacggctctc attcctggta
     2341 tgccggtgct gaagtgggtt accgctatca tgtcacagag gatgcctgga ttgagccaca
     2401 ggcggaactt gtttacggtg ctgtctccgg gaaacagttc agctggaagg accaggggat
     2461 gagcctgtcc atgaaggaca aggactacaa tccgctgatt ggccgtaccg gtgtggatgt
     2521 gggtaaatcg ttctccggta aggactggaa agtgacggca cgagccggtc ttggctacca
     2581 gtttgacctg ctggcgaacg gagaaacggt actgcgtgat gcatccggag agaagcgtat
     2641 caagggcgag aaagacggcc gtatgctgat gtctgtgggt ctgaacgcag aggtccgtga
     2701 caacatccgt tttggtctgg agtttgagaa atcagccttt ggtaagtaca acgttgataa
     2761 tgctgtcaac gccgggttcc gttattcatt ctgacggttg ttactgaagg ggattatggc
     2821 ctgacggctc cctgtaaata aagacaggca cagaagattg tgagttactc agtgttccac
     2881 caaaaagtaa ggtcgcaact ctgtgcctgc ttcgtaagtg tacagtgagg gccatatggt
     2941 cataactgaa gcatccgacg acgtggtgtc taccagagtg tcagatatgc aggaaaatat
     3001 gagttccgac agaaatgcag taaaaaagga gtctcgcact cctctccaga aaactccagg
     3061 gctgtaagtt gtattaaaaa gctgattttt ctttagcgat acagacctca ataatataac
     3121 acccttcagt tactgtggtt atgcacaggt gttattttta acagtgtgga taaacagtgt
     3181 cgattattca gattggttat ggggcatgta tgggacattt ttaatcggcg aaatacgtcg
     3241 aagttcgtcg atatggtaaa cgaatcatct atccaaccct tgaaaaacgg cgctcctgga
     3301 cgatcttcgt cgatttttaa aatgcaacaa gtacgttgtt catgtaatag gctaccttgc
     3361 tacagcaagc tcttgacata actgtcaatg tcgctgatgt atttttctat tcctggaaca
     3421 tccttctcac gaatcagatg ttccagcgtt tcacataact gcttgccggg taccagattt
     3481 agcatggcaa atacgccttt aagacgatga gccgtttggg ctaacgcagc aaagtcactg
     3541 gttgctgctt cagtatatag cctcttaaca tcatccggta ctgtgtctac aaagagcgca
     3601 taatagccgc tggcatggag ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc
     3661 tcttcctgcg ccagttgcac ttcaattaat tgtaagaccg cttcctgcat agcgttgctc
     3721 atattgaagt tgacgcacaa ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg
     3781 cttaaaagca agccagaggc agtaagatta gacggattat ccgttaaaaa gatatcataa
     3841 tcttgactaa ttaatctttc atcgggtgtg atacaggttg ccccccaatt ttctaactgg
     3901 cgagtgacaa tattccgaat ttctgccgaa gtaacatcca ccattacgca gacatcatcc
     3961 agtaatcgct cttcttcctc ttcaacttcc gggtcagctg cgagcatttt gatatgcaca
     4021 gagtagcgtg taccaagccc atcccgcgtt ttgatgttta aatgaccgcc cagtttacgc
     4081 gccagttgat cgctcagcca gaatgccagc gggtccgcct tgccatagcg atcgttttgg
     4141 gtctggttga taaacgggaa gtgcaaatta tccatttcat gaatgcttac gccttctcca
     4201 gtgtccagaa tgcggaacgt caggcggtct tcggaggact catcctgatc aacctcaagg
     4261 gtgatttttc ccaattgcgt tgaggtcacg gcatattgca tcagtagtag caaaatacgt
     4321 cgtaaggcat cgcgatcgcc gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc
     4381 agttgcagac ctttacgctt gatggcaggc aacactgaag gcactacttc atcaattaaa
     4441 tcctgcacgg agaacagctc cgtctcactt ttccagctat cgtccgcaag catgttcgct
     4501 aactgtattt catcgaccag ccgcaccagc acatctgcct gattcgccag ttgtttgcct
     4561 tccggggcgt tgagtttagc cgcgctctcc gccagggact gtgcgggttc tttcagcgca
     4621 tcgccaatgt ttttcataaa ggtcatccgc ccctgctggt ttttctcata cagacgctgc
     4681 gcctgcttga gtttcttgtt taccagcact tcgcgatcct gatcgcgaat aatgaaaatt
     4741 tgtgtgcgcg gcgcaacctg gctgcggaac atgcggatct catacagctc gttattgatc
     4801 gtcgcctgaa taatcccctg atgctgctcc gccatggtag tgatgttttg cagattcaaa
     4861 tgcggcagca aatgatcggc aattttgtta cttatgactg tgcggttcga ttcctgatcg
     4921 tgaaccagca ggccgagcgg cagcagtgag actatctctt cattaattgc ccgtaaaatg
     4981 cgcaattcgt tattgaccgc cgtgctgggg acgctttctg tactgcggct ggagaaatgg
     5041 cggaatgtgg tatagccaaa taatgccagc gccagcaaac cgatgttcag cagcagtggc
     5101 agcagaatgt tttgcaacgt atccagcagt aaggtgccgt aaggaacctg ccagaccagg
     5161 cgcatatcgg tagagttgag tgccgaggag atttcaatct tcgtactgtt aaagtggata
     5221 ctgacgctat ccgtcccttc tttctcattt tcattgtttc ccgttgccgt cgcgtctggc
     5281 tcaaggcgga aactgtccag cggcataccc ggtgggatca aatcattaat cggcagatca
     5341 aaagccacga ccgttgccag atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa
     5401 tgaccgttct gccaggccag gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg
     5461 ttggcctgtt gcaacatctc tgcacgacgt gaatcaacaa tgtcgctgac ggtcgattct
     5521 ttaaatccgg aggtgagatc tttgaggggc agggttgaga tcagcaccag actgttatcc
     5581 tgaccattca ggtaatacat cgaccacggt acattttctg cgccccacaa tgtgtccaga
     5641 taggtggaca tccgttgagt catctcaagc gttgagttgt catgagagcc aaagattaac
     5701 gcttccgttt tgcggcgtgg tttctccaga tagtagacat cctgtttcag gcgcgtctct
     5761 tgtaaacctt cgccggagga gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag
     5821 gtcacgtaac gccaggtatc gacgcgcttt tggattgcgt gggtaatgtc gacaatctgg
     5881 taacttttat ctttcagcca ggcgttaacg gcgctttgta ccattacacc catcgtcacc
     5941 agtaacacaa tgatcaacag taaaaagaag cgggtaatgc tccccggtag gagtgaaaag
     6001 cgggtcgtgg ccgttgtctc tttctgacgc attcgtgttt atgacctgtt aaaacttcgc
     6061 gaagtgatag ggcagtataa agggtacagt gtgaaattcc agactcttac ctgattgatg
     6121 attctctatt ctccagtgat atttatcatc ctgcattggg ggaaatagat gtacaagcgc
     6181 cattttgctg tacaaataac ctacaaaaag cctaacgaaa ttaattgcaa ataaacgaag
     6241 attgctggaa attatgctga tgttaattat ttgtgaaata gttaacgagc gttatagttt
     6301 ttctgtggta gcacagaata atgaaaagtg tgtaaagaag ggtaaaaaaa accggatgcg
     6361 aggcatccgg ttgaaatagg ggtaaacaga cattcagaaa tgaatgacgg taataaataa
     6421 agttaatgat gatagcggaa gatattctag ttgcgagtga aggttttgtt ttgacattca
     6481 gtgctgtcaa ataattaaga ataagttatt gattttaacc ttgaattaat attgcttgat
     6541 gttaggtgct tatttggcca ttccgcaata atcttaaaaa gttcccttgc atttacattc
     6601 tgaaacatct atagcgataa atgaaacatc ttaaaagttt tagtatcata ttcgtgttgg
     6661 attattctgc atttttgggg agaatggact tgccgactga ttaatgaggg ttaatcagta
     6721 tgcagtggca taaaaaagca aataaaggca tataacagag ggttaataac atgaaagtta
     6781 aagtactgtc cctcctggtc ccagctctgc tggtagcagg cgcagcaaac gctgctgaag
     6841 tttacaacaa agacggcaac aaattagatc tgtacggtaa agtagacggc ctgcactatt
     6901 tctctgacga caagtctgta gatggcgacc agacctacat gcgtcttggc ttcaaaggtg
     6961 aaactcaggt tactgaccaa ctgaccggtt acggccagtg ggaatatcag atccagggca
     7021 acagcgctga aaacgaaaac aactcctgga cccgtgtggc attcgcaggt ctgaaattcc
     7081 aggatgtggg ttctttcgac tacggtcgta actacggcgt tgtttatgac gtaacttcct
     7141 ggaccgacgt cctgccagaa ttcggtggcg acacttacgg ttctgacaac ttcatgcagc
     7201 agcgtggtaa cggcttcgcg acctaccgta acaccgactt cttcggtctg gttgacggcc
     7261 tgaactttgc tgttcagtac cagggtaaaa acggtagcgt agatggcgaa ggcatgacca
     7321 acaacggtcg tggtgctctg cgtcagaacg gcgacggcgt tggcggttct atcacttatg
     7381 attacgaagg cttcggtatc ggtgctgcag tgtccagctc caaacgtact gacgctcaga
     7441 atggtactta cgtagttgat aatgtaactc ataactacat cggtactggc gaccgtgctg
     7501 aaacctacac tggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
     7561 cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
     7621 tcgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtccctg gcttacctgc
     7681 agtctaaagg taaaaacctg ggtgtcatca atggccgtaa ctacgacgac gaagatatcc
     7741 tgaaatatgt tgatgttggt gcgacctact acttcaacaa aaacatgtcc acctacgttg
     7801 actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg
     7861 ataacatcgt agctctgggc ctggtttacc agttctaatc tcgattgata tcgaacaaag
     7921 ggcctgcggg cccttttttc attgttttca gcgtacaaac acagtttttt ggtgtactct
     7981 tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc
     8041 tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc aaaacccacg
     8101 ctactgaagt taccgttctt gaaggcaaaa ccatgggtac cttctggcgt gccagcattc
     8161 cgggcattga cgccaaacgc agtgccgaac ttaaagaaaa gattcagacc caactggacg
     8221 ctgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgact
     8281 cgcaaagttt gtcgccgtgg ccggtaagtg aagcgatggc cgatatcgtc actacctcgc
     8341 tgcgcattgg cgcaaggacc gatggtgcga tggatattac cgtcgggccg ctggtgaatc
     8401 tgtggggctt tggtccggaa caacaaccgg ttcaaattcc gagccaggaa cagatcgatg
     8461 cgatgaaagc caaaaccggc ttacagcacc tgacggtcat taatcagtcg catcagcaat
     8521 atctgcaaaa agacctgccg gatttatatg tcgatctctc caccgtcggc gaaggttatg
     8581 cggcggatca cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg
     8641 tgggcggcgc gctgaacagc cgtggtatga acggtgctgg ccagccgtgg cgggtagcga
     8701 ttcaaaaacc aaccgataaa gaaaacgcgg ttcaggccgt ggtggatatc aacggtcatg
     8761 gtattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttctc
     8821 atgttatcga tccgcaaacc gggcgtccta tcgaacacaa tctggtatcc gtgacggtga
     8881 ttgccccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta ctggggccgg
     8941 agaaagccaa agaggttgtt cgtcgggaag ggctggcggt ttatatgatc accaaagagg
     9001 gtgataattt taaaacctgg atgtcgccgc agtttaaaag cttccttatc agcgaaaaaa
     9061 attaaagcgc aagattgttg gtttttgtgc gatggtgacc aggcagacta aaggctatcc
     9121 ttaaccaggg agctgattat gaaatacgcc acatgcttaa ctgacgatca acgctggcaa
     9181 tctgtcttag cccgcgactc gaatgccgac ggcgaattcg ttttcgccgt gcgcaccacg
     9241 ggtatctttt gccgtccgtc ttgccgagcc agacatgcct tgcgggaaaa tgtctccttc
     9301 tacgcaaatg ccagcgaggc gcttgccgct ggctttcgtc cctgcaaacg ttgtcagcca
     9361 gacaaagcca atccccggca acatcgcctt gataaaatta cccacgcctg ccgacttctg
     9421 gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc aggtggcgat gagtccattt
     9481 catttgcatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc
     9541 tggcgcgctc gccgtttgcg cgaattgctg gcgaaagggg agagcgtgac tacatctatt
     9601 attaacgccg gattccccga cagcagcagt tactaccgca aagccgacga aacgctgggc
     9661 atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct
     9721 gattgtgagc tgggccgttg cctggtggca gaaagtgagc gggggatttg cgcgatatta
     9781 ctgggcgatg atgacgcgac actaatcagc gagttacagc agatgtttcc cgctgccgac
     9841 agcgcgcctg ccgatctgac gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat
     9901 caacgcgata cgccgctgac gttaccgctg gacattcgcg gcactgcttt tcagcaacaa
     9961 gtctggcagg cactgcgcac gataccttgc ggtgaaaccg tcagttatca gcaactggct
    10021 aatgccatcg gcaaaccgaa agcggtacgg gccgttgcca gtgcctgtgc cgccaacaag
    10081 ctggctatcg ttataccttg tcatcgggtg gtccgtggtg atggcacact ttccggttac
    10141 cgctggggcg tgtcgcgcaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggaaagg
    10201 taatgttgga tctgtttgct gatgctgaac cgtggcaaga accactggcg gctggagcgg
    10261 tgattttgca ccgttttgct tttaacgctg cggagcaact gatccgcgat attaatgacg
    10321 ttgccagcca gtcgccgttt cgccagatgg tcactccagg cggatatacc atgtcggtgg
    10381 cgatgaccaa ctgtgggcat ctgggctgga cgacccatcg gcaaggttat ctctattcgc
    10441 ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc gcagagtttt catcatttat
    10501 gtcaacgtgc ggctacggcg gcgggctatc cagatttcca gccagatgcc tgtttgatca
    10561 accgctacgt tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc
    10621 gcgcgccaat tgtttctgtt tctctgggct tacccgcgat ttttcaattt ggcggcctga
    10681 aacgaaatga tccgctaaaa agattgttgt tggaacatgg cgatgtggtg gtatggggcg
    10741 gtgaatcgcg gctgttttat catggcattc aaccgttgaa agcggggttt catccactcg
    10801 ccggcgactg ccgctacaac ctgacattcc gtcaggcagg taaaaaagaa taaaaataag
    10861 aattattatt gctgtgcgca cgaagatgtt taaactgcgg gctgtaattc attgtccggg
    10921 ttttctgcat ggaacttctt gtacttgtct ggcggcagta tcgctggcca tttatcagtg
    10981 tgatggcgct aagcctcgcc agtgcggcat taggcattgg cttaattgct tttatcaatc
    11041 agcgccttat cgaaacggcg gataccagtc tgctggtatt gccggagttt ctggggttat
    11101 tgctgctgtt gatggccgtc actctcggat cgcaactggc gctcaccact ttggggcatc
    11161 acttcgttta ccgactgcgt agtgaattta tcaagcggat tctcgatact cacgtcgagc
    11221 gcattgaaca actcggtagc gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata
    11281 tcaccattgc ttttgtgcgt ctgccggaac tggtgcaggg gatcattctc actatcggtt
    11341 cagcggcgta tttgtggatg ctgtcgggca aaatgttgct ggtaacggct atctggatgg
    11401 cagtcaccat ctggggcggt tttgtgctgg tggcgcgggt gtacaaacat atggcgaccc
    11461 tgcgtgaaac cgaagataag ctgtacacgg attttcaaac cgtactcgaa gggcgcaaag
    11521 agctgactct gaaccgtgaa cgcgccgagt atgtgtttaa caacctctac attcctgatg
    11581 cgcaagagta tcgccatcat attattcgcg cagacacctt ccatcttagt gccgtgaact
    11641 ggtcaaacat catggtgctg ggcgctatcg gcctggtgtt ctggatggcg aacagtctcg
    11701 gttgggctga taccaacgtt gccgcgacct attcgttgac gctgttattc ctgcgtacgc
    11761 cgctgctttc ggcggttggc gcattaccga cgctgctgac ggcgcaggtg gcgtttaaca
    11821 agctgaacaa attcgcgctc gcgcctttca aagcagagtt tccgcgcccg caggcttttc
    11881 ccaactggca aacgctggag ctgcgtaacg tgacgtttgc ttatcaggat aacgcgtttt
    11941 ccgttgggcc gattaacctc accatcaaac gtggtgaact gctgtttctg attggtggca
    12001 acggtagtgg aaaatcgacg ctggcgatgt tgctgacggg cttgtaccaa ccacaaagcg
    12061 gcgaaatctt gttggatggc aaacctgtca gcggcgaaca accggaagat tatcgcaaac
    12121 tgttttcggc agtgtttacc gatgtctggc tgtttgatca actgctgggg ccggaggggc
    12181 aacccgctaa cccgcaactg gttgagaagt ggctggcgca gctgaaaatg gctcataagc
    12241 ttgagttaag caacgggcgt attgttaacc tgaagttatc aaaagggcag aaaaaacgcg
    12301 tggcgctgtt gctggcgctg gcagaagaac gcgatattat cctgctggat gaatgggcgg
    12361 cggatcagga tccacacttc cgtcgtgagt tttatcaggt gctgctgccg ctgatgcagg
    12421 agatgggtaa aactattttc gccatcagtc atgatgatca ttactttatc cacgccgacc
    12481 gcctgctgga aatgcgcaac gggcaactta gcgagctgac aggcgaagag cgcgatgctg
    12541 cttcgcgtga tgccgttgcc cggacggcat aatattacga cgctgacctg gcggactgct
    12601 gccggcaggt caatattcta aaaatacctt cgtttctaat aaccc
//
LOCUS       DAZFRE010000125        12604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFRE010000125 DAZFRE010000000
VERSION     DAZFRE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.125"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            267..767
                     /gene="tssB"
                     /locus_tag="ACHKUI_004321"
     CDS             267..767
                     /gene="tssB"
                     /locus_tag="ACHKUI_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6600394.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            826..2364
                     /gene="tssC"
                     /locus_tag="ACHKUI_004322"
     CDS             826..2364
                     /gene="tssC"
                     /locus_tag="ACHKUI_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6600395.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2382..3719
                     /gene="tssK"
                     /locus_tag="ACHKUI_004323"
     CDS             2382..3719
                     /gene="tssK"
                     /locus_tag="ACHKUI_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6600396.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTPGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3716..4381
                     /gene="tssL"
                     /locus_tag="ACHKUI_004324"
     CDS             3716..4381
                     /gene="tssL"
                     /locus_tag="ACHKUI_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO6600397.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4394..6046
                     /locus_tag="ACHKUI_004325"
     CDS             4394..6046
                     /locus_tag="ACHKUI_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6600398.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDAAVLAGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGDEVQVATSTENAMPAQWIAQSGVQE
                     RSQRLCYLLKAESLMQWLNLNVLTALNGPEAKCPPLAMAVGLVTSLPAVDNNLWQVWI
                     TARTGLTTDIADTGTDATLPFPDALLRQLPRQSGFTPLRRACVTMLGITTAAGITMLC
                     LSATANRQLLRHIGDDLHQFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6104..6595
                     /locus_tag="ACHKUI_004326"
     CDS             6104..6595
                     /locus_tag="ACHKUI_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6600399.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6787..9420
                     /gene="tssH"
                     /locus_tag="ACHKUI_004327"
                     /gene_synonym="clpV"
     CDS             6787..9420
                     /gene="tssH"
                     /locus_tag="ACHKUI_004327"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6600400.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAASLCQTRAHAEITIEHWLLKL
                     LEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLIA
                     SLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPAR
                     QPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVFG
                     RDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRTL
                     DLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAANL
                     LKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKAR
                     YAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLTQ
                     LHARLAALDIEREAIEQDCVFCPEASPERLAELTDLRDELQAEAGHLEAQYQQEKALA
                     QQIMTLRQEGTDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKDE
                     QSDLLSMEKSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKTE
                     TALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRPY
                     SVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQLL
                     EEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVARR
                     LRQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHHK
                     PARLRLDWDDEDGIVLEFDEK"
     gene            9432..11942
                     /locus_tag="ACHKUI_004328"
     CDS             9432..11942
                     /locus_tag="ACHKUI_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6600401.1"
                     /translation="MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMAD
                     IISELNGLSDKIQKLSDDATTANADPADMAAQIALITSRINDLTAPVILMHAPKGVAV
                     ASGEHLQLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIESGEYNIQFNFKDDDGIPYANTRYIAFMADGTRKE
                     GITDENGKTDNFNCDKEQEIEVRLLHQSIDMVEGGVNE"
     gene            11935..>12604
                     /locus_tag="ACHKUI_004329"
     CDS             11935..>12604
                     /locus_tag="ACHKUI_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600402.1"
                     /translation="MSDDRGSSTGTAEKKEECVKEFIVSDKFKKMMDDAFNATKSVLK
                     KRAKNLKDWTENDKQEFSQIFGVSGDVIITSTYFAKRVADKLSENVDARTFMIDGVNR
                     MIMICDSISVESRSCQNGVNLYGNFINNTHIFPGSARVNNGITIGLSPDQYKETLRIE
                     ILQNFKKKPFSGRESHVSTLCHELSHFCRYFIDGKHCGGMGTDDVPTEEFDPNFRYTG
                     YARDL"
BASE COUNT         2990 a         3349 c         3628 g         2637 t
ORIGIN      
        1 taaaagtgca aatttgtgaa gcttatcctg ttattatttt aagtttcatg ccaaattctc
       61 tcaccagata atgccgccct cttccgaaaa ataatcaaga ggccaagcaa tatctaaaat
      121 gatacaactg tatctattcc cctgaaaaat acattattta tttgtatatt ttcctcatca
      181 ttgcttttta tttaaatcat ccgataatcc cctgaatata attatgtcaa taaccatcag
      241 aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa gttcccgccg
      301 ctcgtgtaaa tatcaagctt gatctgcata caggcaatgc aaaaaagaaa gttgaactcc
      361 ccctcaagct tcttgccgta ggcgattaca gtaacggaaa agagcagcgt ccgctgtccg
      421 aacgggacaa gattgatatc aataaaaaca acttcaacag cgtcatggct gagttttcgc
      481 ctgcggttaa tttaacagta gaagatacgc taagcggaaa cggtaatgaa caaaatatcg
      541 cgcttgagtt taaaagcctg aaggattttg aacccgaaca ggtagcaaaa aatattccgc
      601 agcttcgtgt attactcgct atgcgcaatt tactacgtga cctgaaatcc aatctgctgg
      661 ataacgcaac gtttcgccgt gaactggaaa atatcctgaa agacccgacg ctcagttcag
      721 aactgcgtga tgaactggcg aaaattgcgc cgcaggagaa cgtttaaccc gaacgcgtat
      781 tcaggttaaa ccggagaaga aaggatcact gaaaaggaaa tgctgatgtc tgtacaacaa
      841 gaacatgcca cctctgaaac tgcaacactc accaccactg agtccggcgg cgtttatcag
      901 tccctgttcg ataaaattaa tttaaccccg gtctcttcca ttcaggaaat cgatttatgg
      961 caaaacagcg aaacgctggc cgatgcctca cccgatgagc gcgtgacggc ggcgattcac
     1021 gttctgcttt cctgtctggc gaaatcaggc gaggacgtgg ttaagctcga caagagcctg
     1081 ctggattttc atatcgacga tctggatcag aaaatcagta aacagcttga tgcggtcatg
     1141 caccaccctg aattccagaa agtcgagtcg ctgtggcgtg gtacatggtt cgtcgtacag
     1201 cgcactgatt ttcgcaaaaa tgtcagaatt gaattgctgg atatcagtaa agaacatctg
     1261 cggcaggact ttgatgattc tccggaaatc attcagagtg gtttatatcg ccatacatac
     1321 attcaggaat atgatacgcc gggtggcgaa cctgtagcct cattaatatc cagctatgaa
     1381 tttgataaca gcccgcagga tattgccctg ctgcgtaata tttccagagt gtctgccgct
     1441 tcccatatgc cttttatcgg ttctgtcgga ccgaaattct tccttaaaaa ttcgatggaa
     1501 gaagtcgccg cgattaaaga tatcggcaac tactttgacc gcgcagaata tattaaatgg
     1561 aagtcgttcc gcgatacgga tgacagccgc tatgtgggat tagtgatgcc gcgcgtgctg
     1621 ggccgtctgc cctatgggcc ggacacggtg ccggtacgca gctttaacta tgtggaagaa
     1681 gtcaaaggcc cggatcacga aaagtacctg tggacaaacg cctcgttcgc ctttgccgcc
     1741 aatatggtga agagctttgt gaataatggc tggtgtgtgc agatccgtgg tccacaggca
     1801 ggtggcgcag tggccgatct gcccatccat ctttacgatc tcggcaccgg caatcaggtc
     1861 aaaattccgt ccgaagtgat gatcccggaa acccgcgaat ttgaatttgc caaccttggc
     1921 tttattccgc tctcttatta taagaatcgc gattacgcct gcttcttctc ggcaaactcc
     1981 gcccagaaac cggcgttgta tgataccgct gacgccaccg ccaacagccg tattaatgcc
     2041 cgtttgccct atattttcct gctgtcccgc attgcgcatt acctgaaaat catccagcgc
     2101 gagaatatcg gtaccaccaa agaccgccgc gtactggaac tggaactgaa cacctggatc
     2161 cgcacactgg tgacggagat gaccgatccg ggtgatgaac tgcaggcgtc tcacccgctg
     2221 cgcgacggca aggttatcgt ggaagatatt gaggacaatc cgggcttctt ccgcgtcaga
     2281 ctctttgccg tgccgcattt ccagattgaa gggatggata tcaacctgtc actggtttcc
     2341 cagatgccga aagcgaaagc ctgaccgggc ggtgatgtga tatgaaaatt catcgaccac
     2401 tgtgggctga ggggacattc ctgtcctctc agcagtttca gcagcaggcc cgctgggaag
     2461 cgttcagcaa tgacagcatt gcgcaacttt gtattcgtca tccgtggggg atcgcaaacg
     2521 tgctctttga ccgggatgcc ctgacgcccg gaaagctgaa aacgcaggca gtacgcctgc
     2581 gttttgctga tggtacgctg atcgattcag acgtggcgga tattctgcct cctgcctgtg
     2641 acctgcgcac gctggagggc ggcagcgcga ttgttttgct ggcgctgccg cttgcgcatg
     2701 gtaacggcgg taatctcggt cagggcgagc aggctgaaag gccgctgcgc tatcgtcagg
     2761 aatggcaaaa ggtgcaggat atctacggca gtgacagtga agatatggcc gttgagcgcc
     2821 atgcactgtc cctgcgcttt gcccatgata acaatcagga ttacatcacc tgtccgctgg
     2881 cgcgtctggt gcgggatgtt cagggcaact ggacgcagga tgagggctat attccgccgt
     2941 tactggcgtt taacgcccat gacgggctgg tacagcgtct ggacacactg ctgttgcagc
     3001 tacgggcgaa gtgccagcgt ctgatggcga tgcgccggga aagcaaccag cgtatggcgg
     3061 actttgccgt cgccgatgtc tccctgttct ggctgcttaa cgcgctgaac tccgccgagc
     3121 cggtactgag tgatttcctg cgctacccgg cggttcatcc cgaactggtc tggcgggagc
     3181 tggcgaggct tgccggtgcg ctgctgacgt tttcccttga acataacgtc agcgccgttc
     3241 cgccctatgt ccacgaatcg ccgtccatcg tctttccgcc acttttctcc ctgctcagcg
     3301 aactgctgga agccagcctg ccgtcacggg tgatcgcgct ggatctggcg tccctgccgg
     3361 gcaaccgctg gaaagcagac ctgcacgatc cgcgcctgcg ggaagaggcc gacttttacc
     3421 tgtccgttcg ttcctctctg cctgcgcatc aggtactgca ccagctaccg ctggtctgca
     3481 aaatcggtgc gcctgatgac gtcacgctgc ttatcaatgt ggcgctgaat ggtgttccac
     3541 tggtgccgct tacctcagtt ccggcagcgc tgccactgcg gctggagaac cagtattttg
     3601 cgctggatat gcacagtgac gcggctaaat cgatgctgga gtccgggtgc tgcatgattt
     3661 atgcgccggg aaccatgggc gacctgaaac ctgaactgtt tgcggtgctg cgcacatgaa
     3721 aaaagagacg gatatcaata tcgacgcgct gctgcgcgat acgtttctga cggtggtgga
     3781 actgcgtcag ggaaccaccg tgcgtcacgg tatggagctg tatcgccact gccagcggca
     3841 ggtggaactg gtccgcgaac gcctgaaaga tgccgggttc agccgggaga gcgtggagca
     3901 cattacttac gcgcagtgcg ccctgctgga cgaaacggtc ctgagccgtg gcggcatgga
     3961 cgacggtcag gccatctgga tgaaggatcc actccagtcg catttcttca acactctgca
     4021 ggcaggcgag ctgctgtacg aacggatgaa gcaggtgttg caggagcccg cccccgcgca
     4081 ggcagtgctg acctgctttc accgtgtcct gctgctggga tttcgcgggc gttatcagga
     4141 tccggtggcc ccggagcgtg accagcttat ttcgacgctc aacgggctgg tcgcgccatt
     4201 tggcgtgctg ccggaaacgg cggtactgaa cgtgccgtta tccccccgtc agcatccttt
     4261 gctgcattcg ccgtttttct ggctggtaac gctgacgctg ctactggccg gtgtctggtg
     4321 gggactgcac cactggctga acgtgctggt ggatgaactg ctgccgcaaa gcctgcgtta
     4381 aagggagccg tgagtgagga acacgctgaa acaggccatc gtgctgtggg gaatggtgtt
     4441 actgctggtg ctgtggtggg tgtttgtcag tccgtctggc gtactgagat gggccggtgc
     4501 agcagttatc gttctggcgg ttgccgcatt gttgatttac cggcgcaggc agacgtggac
     4561 ggagatgacc ggcgatgccg ggttgttatc gctgccgccg gaaacgtacc gacaaccggt
     4621 agtgctggtc tgtggcgata tgtcggcgca tctgttcact gacagcccgg tccggcaggt
     4681 ttcagaaggg ctgtatctgc atgtttctga tgaagaacag cttgtggcgc aggtggagcg
     4741 attactgacc cttcgcccgg cgtgggcatc gcagcttgcc gtggcgtata ccgtcatgcc
     4801 cggcatgtac cgggatgcgg cggttctggc cggacaactg cgccggttcg cccacagtat
     4861 ggcgacagtg cgtcgccggg cgggcgtaaa cgtcccctgg cttctctgga gcgggctgtc
     4921 cggctcgccc ttgccggaga gagcgaattc accgtggttt atctgtaccg gagacgaagt
     4981 tcaggtagca acatccacag agaacgcgat gcccgcgcag tggattgcac aatccggcgt
     5041 acaggagcgc agtcagcgac tctgttacct gctgaaagct gaaagcctga tgcagtggct
     5101 gaatcttaat gtgctgacgg cactgaacgg cccggaggcg aaatgcccgc cactggcgat
     5161 ggccgtgggg ctggtcacct cgttgcctgc ggtggataac aacctgtggc aagtgtggat
     5221 caccgccaga accggcctga cgacggatat cgcggacacc ggcacagacg ccacgctgcc
     5281 gtttccggat gccctgttac ggcagttgcc gcgtcagtcg ggctttaccc cgctgcggcg
     5341 ggcctgcgtg accatgctgg gcattaccac cgcggcgggt atcaccatgt tgtgcctgtc
     5401 agccacggca aatcgccagt tattacggca tatcggtgac gatctgcacc agttttatgc
     5461 cgttccggcg gaggaattta tcaccaaagc ccgtcacctg tcggtgctga aagacgatgc
     5521 ggtcatgctc gatgggtatt accgggaagg agaaccgctg cgtctcggtc tggggttata
     5581 ccccggtgaa caaatccgcc agccggtatt acgcgccatt cgcgactggc gtccgcctga
     5641 gcaaaaaatg gaagtgacag cctcgcttca ggcgcagacc gtgcgtcttg acagtatgtc
     5701 gctgtttgac gtcggacagg cccgcctgaa agacggctcg acaaaagtgc tggtggacgc
     5761 actggtgaac atccgggcaa aaccgggctg gctgatcctc gtggccggat ataccgacgc
     5821 caccggcgac gaaaaaagca atcagcagtt atcgctgcga cgtgccgaag cggtgcgtaa
     5881 ctggatgctg cagaccagcg acatcccggc cacctgtttt gccgtacagg gactgggcga
     5941 gagccagcct gcggcgacca acgacacgcc acagggccgg gcagtcaacc ggcgtgtcga
     6001 aatcagtctt gttccgcgtt ctgacgcctg tcaggacgtg aaataaaaca taccgccgga
     6061 agaaggcggt gcttcaatca cactaacaag gagagtaatt ctcatggcta ttcctgctta
     6121 tctctggctg aaagatgacg gcggcgcgga tatcaaaggt tccgtggacg ttcaggggcg
     6181 cgaaggtagc atcgaagtgg tggcgctgga tcacgatgtg tacatcccga ccgacaacaa
     6241 caccggcaaa ctgaccggta cccgtactca caagcccttt acgtttacca aagaaatcga
     6301 tgcctccagc ccgtatctct acaaagctgt gaccaccgga cagaccctga aaacggcaga
     6361 atttaagttt taccgcatca acgatgccgg tcaggaagtg gagtacttca acatcacgct
     6421 tgataacgtc aagctggtca gagtcgctcc gcttatgcac gacatcaagg atccttccag
     6481 agagaagcat aaccacctgg aacgtattga gttccgctac gagaaaatca cctggactta
     6541 caaagacggc aacatcattc attccgactc gtggaatgag cgtccttccg cctgataact
     6601 gcggcagaca ggaaactgtc tgcctttctg ttgtgtgctt tgtccagtgc cgcaggcacc
     6661 ggtactgagc aaagcatacc cacaatctgg tgaaacctga tgggcctgag tgaggtaacg
     6721 gctatgggcg gtagcgtgcc tgaacgcgga tcgcaggaat acagaacacg ataaggaaac
     6781 ccttctatga caggaaatca ccccgccgcg ctgctgcgtc gccttaaccc atactgtgca
     6841 cgggcgctgg acgccgcctc gctgtgtcag acccgcgccc atgcggaaat aaccattgaa
     6901 cactggctgc tgaaactgct ggagcaggga gaaggcgata tcacggtgat tgcccgccgc
     6961 tatgaatggg atatcgacac gctctggcag tctctgctgg cacatctgga caccttaccc
     7021 cgcccggtcc gcgaacgtcc tcaactttct gaaccgctgg cagcgcttat ccgacaggcg
     7081 tggctgatag cgtcactgga aggcgacgat ccacaaatcc gcagccagca tctgctgatg
     7141 gcgctgacag aaaaaccgat gctgcccgcc tgtaatgacc tgtgggtatt gctgagtctg
     7201 agccgcgtgc agcttgagcg gctgcgtccc ctgctggatg cgcagtcgga tgaatgtccg
     7261 gcacgtcagc cacaggtcac cgaaccgctg acctctgcac tgccggagac ggcaacggcg
     7321 gacgcaccgg caaaaacgct gacggagaaa caggatgacg ccctgctggc ggtgcttaac
     7381 cgctttaccg aagacgtgac ggaaaaagcc cgcagcgggc gaatcgaccc ggtattcggg
     7441 cgcgacacgg aaattcgcca gatggtcgat atcctctccc gtcgccgcaa aaacaacccg
     7501 attctggtgg gagaaccggg tgttggcaaa accgcgctgg tggaagggct ggcgctgcgt
     7561 atcgccgaag gcaacgtgcc ggacagcctg aaaacggtgc atatccgcac gctggatttg
     7621 gggctgttgc aggccggggc gggtgttaaa ggtgaatttg aacagcggct gaaaaacgtg
     7681 attgacgcgg tgcagaaatc gccggagccg gtgctgctgt ttattgatga agcccatacc
     7741 attatcggcg cggggaatca ggcaggcggc gcggatgccg ccaacctgct gaaaccggcg
     7801 ctggcaaggg gcgaactgcg caccatcgcc gccacaacgt ggagcgaata caaacagtat
     7861 tttgagcgcg acgccgcgct ggagcgccgc ttccagatgg tgaaagtgga cgagccggat
     7921 gatgacaccg cctgcctgat gctcaggggg ctgaaagccc gctatgcgca gcaccacggc
     7981 gtacatatgc tggacagcgc cattcagacc gccgtgcgcc tgtcgcgccg ctatctgacc
     8041 ggacgccagt tgccggacaa ggcggttgat ttgctggata ccgccggagc aagagtccgc
     8101 atgagcctcg acaccctgcc ggaaccgttg acgcagcttc atgcgcgact ggcggctctg
     8161 gatattgagc gggaagcgat tgagcaggac tgcgtgtttt gtcccgaagc cagcccggag
     8221 cggctggcgg aactgaccga tttgcgtgat gagctacagg cagaagccgg gcatctggaa
     8281 gcgcagtatc agcaggaaaa ggcactggcg cagcagatta tgacgttgcg tcaggaaggg
     8341 acagacagca ctgaactgca acagcaactg cgaacgcatc agggctttgc accgctgctg
     8401 gcgctggatg tggacgcccg cgccgtcgcc acggtggtgg cggactggac cggcatcccg
     8461 ctctcttccc tgctcaaaga cgagcagagc gacctgctca gtatggaaaa gagccttgaa
     8521 aaccgcgtgg tcgggcaaag cccggcgctc tgcgccatcg cacagcggct gcgggcggct
     8581 aaaaccggcc tcacgccgga gaacggccca cagggggtat tcctgctgac cggccccagc
     8641 ggcaccggta aaaccgaaac tgcgctcaca ctggccgaca ccctgtttgg cggtgaaaaa
     8701 tcccttatca ccattaatct ttcggaatat caggaaccgc ataccgtttc ccagttgaaa
     8761 ggttcgcctc cgggctatgt cggctacggt cagggcggcg tgctgaccga agcggtacgc
     8821 aaacgccctt acagcgtggt gctgctcgac gaagtggaaa aggcgcatcg cgacgtgatg
     8881 aacctgttct atcaggtgtt cgaccggggc tttatgcgcg acggcgaagg gcgggaaatc
     8941 gacttccgta acaccgtgat tctgatgacc gccaatctgg gcagcgacca catcatgcag
     9001 ctgctggagg aaaaaccgga cgccacggac gcagacctgc atgaactgct gtaccccctg
     9061 ctgcgagacc attttcagcc tgcactgatg gcgcgctttc agacggtgat ttaccgcccg
     9121 ctgggacagg aggcgatgcg cgccattgtg gaaatgaaac tggcgcaggt ggcccgccgt
     9181 cttcgccagc actatgggct ggaaacggaa atcagtaaca gcctgtacga cgccctgacc
     9241 gccgcctgcc tgctgccgga caccggtgcg cgtaatatcg acagcctgct gaaccagcaa
     9301 atcctgccgg tcttaagcca gcagttgctg gcgcagcagg ccgtgcatca taagcctgcc
     9361 cgactgcggc ttgactggga tgatgaagac gggattgtgc tggaatttga tgagaaataa
     9421 tggaggctct tatgaacgta cagttcttcg accacgcaca ccacaagctc aaaattcgcg
     9481 gcctgcaaag tccggtggat gtgctgacct ttgaaggccg cgagcaactg agtaccccgt
     9541 tccgctacga cattcagttt accagcagcg ataaagccat cgcgccggag tcggtgctga
     9601 tgcaggatgg cgcattcagc ctgacggcac cgccggtaca gggaatgccg gtacagactg
     9661 ccctgcgcac cctgcacggc gtcattaccg gctttaaaca tctctcatcg tcacaggacg
     9721 aagcccgtta cgaagtgcgc cttgaacccc gtatggcgct gcttacccgc agccgccaga
     9781 acgccatcta ccagaaccag acggtgccgc aaatcgtcga aaaaatcctg cgcgagcggc
     9841 accagatgcg cggtcaggac ttcgtgttta acctcaaaag cgaatacccg gcccgcgaac
     9901 aggtgatgca gtacggtgag gacgacctga cgtttgtttc acgcctgctg tcagaggtgg
     9961 gtatctggtt ccgctttgcc accgacgccc ggttgaaaat cgaagttatc gaattttacg
    10021 acgaccagag cggctacgag cgcgggctga cgctgccgct tcgccatcct tctgggctgt
    10081 ttgatggtga aacggaagcc gtatgggggc tgaacaccgc gtacagcgtg gtggaaaaaa
    10141 gtgtctccac gcgggactac aactaccgtg aagccacggc ggaaatgacc acaggccagc
    10201 atgacgccac cgggggcgat aacaccactt acggcgaagc ctaccactat gccgacaact
    10261 tcctgcaaca aggcgataaa gaggcagcgg aaagcggcgc gttttatgcc cgtatccgtc
    10321 acgaacgcta cctgaatgaa caggccattc tcaaaggcca gagcaccagt tcactgctga
    10381 tgccggggct ggaaatcagg gttcagggcg acgacgcgcc agcggtgttc cgcaagggcg
    10441 tgctgattac cggcgtgacg accagcgcgg cgcgtgaccg cagctatgag ctgacgttca
    10501 cggcgatacc gtactcagag cgctacggct accgtccggc gcttatcccc cgccccgtaa
    10561 tggccggaac gctcccggcg cgggtcacca gtacggtgaa gaacgacatc tacgcccata
    10621 tcgacaagga cgggcgctac cgggtcaacc tcgactttga ccgcgatacg tggaaaccgg
    10681 gctatgagag cctgtgggtg cgccagtcgc gcccgtatgc gggagacacc tacggcctgc
    10741 acctgccgct gctggcagga acggaagtca gtatcgcctt tgaggaaggc aacccggacc
    10801 gaccttatat tgcaggcgta aaacacgact cggcacacac tgaccatgtg accattcaga
    10861 actacaagcg caacgtactg cgtaccccgg cgaacaacaa aatccgcctc gacgatgagc
    10921 gcgggaaaga gcatatcaag gtcagcaccg aatatggcgg caagagccag ttaaacctcg
    10981 gccatctggt ggatgccggg aaacagcagc gcggggaagg ttttgaactg cgtaccgatc
    11041 tgtggggcgc ggtgcgggcg aagaaaggca tttttatcag cgccgatgcg caggacaagg
    11101 cgcagggtca ggtcagagag atggccgaca ttatcagcga actgaacggt ctgagcgata
    11161 aaatccagaa actgtccgat gacgcgacga cggcgaacgc cgacccggcg gatatggccg
    11221 cgcagatagc attgataacc agccggataa atgacctgac cgccccggtg attttgatgc
    11281 acgcaccgaa aggcgtggcg gtcgccagcg gcgagcacct gcaactggcc gcagtgaaaa
    11341 acctccagat aaatgcggga aacaacgccg atatcggcgt ggtgaagaat atgtttattg
    11401 gcgtggggcg ggcgctgagc gtgtttgtgc gcaaggcggg tatcaaactg tttgccaata
    11461 aaggggctgt ctcggtacag gcacagaacg atctgatgga actgttggca cagaaatcga
    11521 tagagatcac cagtacggaa gatgagatca agatcaccgc gaagaagaaa atcaccctta
    11581 acggcggtgg cagctacatc cggctggatg cctgcgggat agaagcggga acgccgggag
    11641 aatataacgt taaggccgga tattacgggc gtaaaccaaa ggcgaagctg acgccggagc
    11701 tgatggcgtt tccggtgatt gagagcgggg aatataatat tcagttcaat ttcaaggatg
    11761 atgatgggat accttatgca aatacgagat atattgcgtt tatggccgat ggaacaagga
    11821 aagaaggtat taccgatgag aatggaaaga ctgataattt taactgtgat aaagagcagg
    11881 aaatagaggt caggttacta catcagagca ttgatatggt tgagggtggt gtaaatgagt
    11941 gatgatcgtg gtagttcaac aggtacggca gaaaagaaag aagaatgtgt taaagagttt
    12001 attgtttccg ataagtttaa aaaaatgatg gacgatgcct ttaatgcaac taaaagtgtg
    12061 ttaaagaaaa gggctaagaa tctgaaggat tggactgaaa acgataaaca ggaatttagt
    12121 caaatatttg gtgtttctgg tgatgtaatt attaccagta catattttgc gaagagagta
    12181 gcagataaat tatcagaaaa cgttgatgct cgaaccttta tgatagacgg tgttaatcga
    12241 atgattatga tatgtgatag tatctctgta gaatcacgct catgccaaaa tggggtgaac
    12301 ttatatggta actttatcaa caatacacat atttttcctg gttcggctcg cgttaataat
    12361 gggataacta tagggctgtc tcctgatcaa tataaagaga cgctacgaat tgagatactt
    12421 caaaatttta agaaaaagcc tttttctggt cgtgaatctc atgtgtctac tctatgccat
    12481 gagttatctc atttttgtag atattttatc gatggtaaac attgtggcgg aatgggaact
    12541 gatgatgttc caacagaaga atttgatcca aacttcagat atacaggata cgctcgagat
    12601 ttag
//
LOCUS       DAZFRE010000126        12335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFRE010000126 DAZFRE010000000
VERSION     DAZFRE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.126"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            430..1134
                     /locus_tag="ACHKUI_004330"
     CDS             430..1134
                     /locus_tag="ACHKUI_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO6600403.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYLFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMGALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            1221..1391
                     /locus_tag="ACHKUI_004331"
                     /pseudo
     CDS             1221..1391
                     /locus_tag="ACHKUI_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            1381..1887
                     /locus_tag="ACHKUI_004332"
     CDS             1381..1887
                     /locus_tag="ACHKUI_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFO6600404.1"
                     /translation="MLSKLRRWLREGAILLVLLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            2070..2885
                     /locus_tag="ACHKUI_004333"
     CDS             2070..2885
                     /locus_tag="ACHKUI_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFO6600405.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            3232..5118
                     /locus_tag="ACHKUI_004334"
     CDS             3232..5118
                     /locus_tag="ACHKUI_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HFO6600406.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALAQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            5159..5686
                     /locus_tag="ACHKUI_004335"
     CDS             5159..5686
                     /locus_tag="ACHKUI_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HFO6600407.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            5790..7169
                     /locus_tag="ACHKUI_004336"
     CDS             5790..7169
                     /locus_tag="ACHKUI_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HFO6600408.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            7172..8455
                     /locus_tag="ACHKUI_004337"
     CDS             7172..8455
                     /locus_tag="ACHKUI_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6600409.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            8445..9575
                     /locus_tag="ACHKUI_004338"
     CDS             8445..9575
                     /locus_tag="ACHKUI_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6600410.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALFAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKLVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            9580..10275
                     /locus_tag="ACHKUI_004339"
     CDS             9580..10275
                     /locus_tag="ACHKUI_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6600411.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            10262..10747
                     /locus_tag="ACHKUI_004340"
     CDS             10262..10747
                     /locus_tag="ACHKUI_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFO6600412.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAAFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPSELEATFARLAA
                     R"
     gene            10772..11257
                     /locus_tag="ACHKUI_004341"
     CDS             10772..11257
                     /locus_tag="ACHKUI_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600413.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            11379..12012
                     /locus_tag="ACHKUI_004342"
                     /pseudo
     CDS             11379..12012
                     /locus_tag="ACHKUI_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
     gene            complement(11956..12180)
                     /locus_tag="ACHKUI_004343"
     CDS             complement(11956..12180)
                     /locus_tag="ACHKUI_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600414.1"
                     /translation="MGEISVKTGNNTRNGGKNSLNRLIRCVCGTKIGPDPRNFNQRVS
                     LGRHDLLIRTFPKGVFGFGQRTLLFFFAGR"
     gene            complement(12162..>12335)
                     /locus_tag="ACHKUI_004344"
     CDS             complement(12162..>12335)
                     /locus_tag="ACHKUI_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6600415.1"
                     /translation="SIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLF
                     RVDQMIRQWERSQ"
BASE COUNT         2737 a         3085 c         3658 g         2855 t
ORIGIN      
        1 gctaacgcgg agatacgccc cgacttcggg taaaccctcg tcgggaccac tccgaccgcg
       61 cacagaagct ctctcatggc tgaaagcggg tatggcttag cagggtggga atgggatagg
      121 cgaaatctat caatcagtac cggcttacgc cgggcttcgg cggttttact ccggtatcat
      181 atgaaacaac agagcgccgc cttccatgcc gctggcgcgg catatcctgg acgtatagga
      241 aattgaaaaa acaatcgata ccattgtcca cgccttatgc cacgtgaacc tttctgcttt
      301 tcccaccact ccggtggttt ctctctctgt atggtcgatt tttataccga aacaggctgt
      361 tttttaggcg ctgttgcaaa tagtcggtgg tgataaactt atcatccctt tttgctgatg
      421 gagctgcaca tgaacccatt caaaggccgg cattttcagc gtgacatcat tctgtgggcc
      481 gtacgctggt actgcaaata cggcatcagt taccgtgagc tgcaggagat gctggctgaa
      541 cgcggagtga atgtcgatca ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa
      601 atggaaaaac ggctgcgctg gtactggcgt aacccttccg atctttgccc gtggcacatg
      661 gatgaaacct acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc
      721 cggggccgca ctgtcgattt ttatctctcc tcccgtcgta acagcaaagc tgcatacctg
      781 tttctgggta aaatcctcaa caacgtgaag aagtggcaga tcccgcgatt catcaacacg
      841 gataaagcgc ccgcctatgg tcgcgcgctt gctctgctca aacgcgaagg ccggtgcccg
      901 tctgacgttg aacaccgaca gattaagtac cggaacaacg tgattgaatg cgatcatggc
      961 aaactgaaac ggataatcgg cgccacgctg ggatttaaat ccatgaagac ggcttacgcc
     1021 accatcaaag gtattgaggt gatgggtgca ctacgcaaag gccaggcctc agcattttat
     1081 tatggtgatc ccctgggcga aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt
     1141 tgaataagac aaaaggctgc ctcatcgcta actttgcaac agtgcctagt acaataattc
     1201 tctatatcca aactgacccc gatgagaaaa gcatggagac gttgagcacc aatctgcaac
     1261 tcgcccggct ggttggcgtt caggggacgc cggcaaccat cattggcgac gagatgatcc
     1321 ccggcgcggt atcgtgggag acccttgagg cggtggtgaa agaaaaactg gcggtggccc
     1381 atgctcagta aactgcgccg ctggctgcgt gaaggcgcga tcttactggt tctgctggct
     1441 ggcgtgataa tcctgctgga tgtctggcgt tctccgcaga tgccggccat gtttgacagc
     1501 acgccgctgc atacgctgga cggggaaacc gtaacgctgg cgtcgatcag tgaagaacgc
     1561 ccggtgctgc tctacttctg ggcgagctgg tgcggtatct gccgtttcac cacgccggat
     1621 gtcgcccgtt tgcagtcgga aggggagagc gtcatgacca tcgcgctgcg ttccggtaat
     1681 gacggggagg tttcccgctg gttatcgcgt aaaagggtga cgttccctgt cgtgaatgat
     1741 agcggcggtg agatatcacg aaactgggaa atcagcgtca cgccaacgct ggtggtggtg
     1801 tcgaaggggc aggtggtaac tacgaccagc ggctggacca gctactgggg catgaagtta
     1861 cgtctgtggc gggcagccat gttttgatag cgtgccgggg aaacccggca ttttttaacc
     1921 cattgtaagg atgtgtaaaa cgagcggaga gcgtgttttc tgttattttt gctgcttttt
     1981 taccgacgcc agttattatt ttttaccgct gcctgacgtt gcaaacctga attgataatg
     2041 gcatatctgt tttaattgaa tgaaagtctg tgatacgttc aggcgatttt ttcgatttcc
     2101 attttgacag ggataacaat tcaatgcgca agggtaaaat tattgttgca tcaatgatgg
     2161 tgctttgtgt ccttattttt gttgcggcgg cggcatggtt tatgctgttc cagggagaaa
     2221 tgacggagcc gacagaggag gaaaaacagg cggcaatttt acacgctgcg ggcctgatga
     2281 aatcagagac ccaggataaa aaatcagtcg aaacgctcta tcatcgttat attattcaac
     2341 gccatgttaa tttagattcc ggcgaactgg tggctggcag cagcgcggac accgcgcggc
     2401 agaagtgtga aaaacttgcc cctgaacgcg acccagcgca ggttcgtcag cgctgtacgg
     2461 tggcggatgt gttctttgtg aaagataaaa ataatgaaat tcagcaggtt atcattccag
     2521 ttaccggtaa gggcgcgaaa tccatgatgc atgcgtttct tgctctgggg ctggacggtc
     2581 gtacggtgag aaatctgtat tattatcagc aacgggaaac gccgtttctg ggggctaggg
     2641 tggaagacgc caactggcgc aaacagtggc cagggaaacg attattagat aacagcggac
     2701 atcctgcatt gaaaatagta caggataaac ctgaacatgc cgatgaatat accgttgacg
     2761 gtatttccgg tgcgacgttg acatcaaccg gcgttgagaa aagtattaac tactggatgg
     2821 ggccgcaggg atatggtcaa ttcttacagc gtcttgccag cgatcgaaat aaccttaatc
     2881 tttgaatatt gctgtacgca ataacgcgtt ttatttctgc ctgccgataa tgaataatat
     2941 cactgtcggc agggggattt attcctctgt taccgcgcct gccttgccgt taattccatg
     3001 agaaaaacag ggagataacg tcccttttat caacgcataa tgtgtgtgtt catcacgatt
     3061 tgatgaaata aatttacata acttgtcgtc tgctgacatt tttatgatcc aggtcatgta
     3121 caacgcgaca aaaagatatg ataataatta tcatttgtat tctttggcgg tgtgcacgtg
     3181 cgtaacttcc gtaactcttt gttggttact ctctttggct tgtttataag catgagtgtc
     3241 tgggcgtcga cgaattatgc gcctttgatt gaagatatcg agcaacgtct tgataaaaca
     3301 gcggaacttt atcagcaaca gcatgccgac gaggcgcgcc gcacggtaca aatggcctat
     3361 tttgaggtgt tcgagaacct ggaagggccg atccgcatca acatttccgc gcgcaaaagt
     3421 tacgagatgg agagcgcatt cggcgagatc cgtcgaatga ttggcgagaa aaaaccgctc
     3481 gctgacgtcc aggcgcgcat tgactggctg aaagccgcac tgcgcgaagt ggaaccggtg
     3541 ctggatggcg gacatcgtct ggtggccgaa gagcagcaca acgcgctgtc cagggacgac
     3601 attgccgttc actggcagga gagtttcagg acgatcgacg atctgctggc gcaggcggtc
     3661 actgaatatc aggccgggaa ttacagcgtt gccagtcagc acgttcaaca ggcgcactat
     3721 cagggcttta aaaactctga gatggagatg tccctcagac aaaaccgttc tgcaaaagat
     3781 gccgcctcca ttaaccaaca attctcatcc ctgattgcgc tgaccgcgca acctgaccat
     3841 ctcaacgatg tctcctatca ggtcactacg ctgctacagg atatcgagga tatcttaccc
     3901 ggcttgccaa ccacccgtga cgatcagcag gttgccgcag cgcaaagcgc agacaacatt
     3961 ccggcggcgg acgagggaaa taaccgcacc gactgggctg aggtgacgac gggaattaac
     4021 cagagcattc aggacgcgat agcgcgctat cagggcggcg atgcgaagaa tgcgattctg
     4081 gatatccagg atacctactt cgaccgcttc gaagccagcg gcatggaaaa caaaatcggc
     4141 tctcgcgatt cggcatttaa aaccacgctg gaggcgtatt ttacccgcct ggtcagtctg
     4201 atgaaggccg gtcagccggt tgagcgcctg aatgcagagg ccagcgcgct ggcgcaggat
     4261 ctgcaaaaag ccgtgacgat gctgggcgaa ggcgaagaaa cccagtggag tctgctgctc
     4321 tatagtctga tgatcatcgt gcgcgagggg ctggaagcgc tgctgattgt tgcggcaatc
     4381 gtggcctaca tggtgaaaaa caaccatcag gataagctgc cgcttattcg tcagtcggta
     4441 attgtggcgc tgatcgccag cgtcatcacc gccgctatct tccagatgct gtttactaac
     4501 tccggcgcca gccgtgaact gctggaaggc ataacgatgc tgattgccgt cgtgatgctg
     4561 ttctttatga gttactggct gctgtccaaa gtggaagcgc gccactggaa agcctggctg
     4621 gaaggcaaac tgtctcattc cctgtcgcga ggctccctgg tcggactatg gctgaccagc
     4681 ttcctggcgg tgtaccgcga aggggcggaa acggtgctgt tctattacgc tctgattggc
     4741 gatgcaaaca cggtatccgg ccatatggcg attggcgccg gttttgtgat tggctgcgtg
     4801 gtgctgctgc tcgcctggct ggtgatgcgt tactcagtgg tgcgcctgcc gctgaaaccc
     4861 ttctttatgt ttaccggcag tttcatgtat ctgatggcct tcgtctttgc gggcaaaggc
     4921 gttctggaac tggtggaagg caaactgttc cagcctacgt tgattaacgg cttccctgaa
     4981 atcagctggc tggggattta tccctacgtg gaaacgttgt taccgcaggt cgtattgctg
     5041 ctcgcggcgc tggtggcgct gtgggtgatg cggcgcaaaa gcgcggttcc cggggagacg
     5101 ataaaaaata acccataatc gttgtatagc cgttgtttta gacgagagga tacgtttgat
     5161 gaccatgaag aaaaccctga ttgccagcgc agtgatggcc agcattttca ttgcgccagc
     5221 cgcgtttgct tttaaagagt acccggcagg cgagcctgtc accatgaatg aaatggagct
     5281 ggcggccgtt tacctgcaac cgattgatat ggaaccgcgc ggcatgggct taccggcagc
     5341 aaaagccgat gttcaccttg aagcggatat ccacgctgta gaaggcaata aaaacggctt
     5401 tggcgcaggg gaatggatcc cgtacctgac catcagctac accctggtca acaacgacac
     5461 gggcgaaaaa caggaaggca ccttcatgcc gatggttgcc agcgatggcc cgcactatgg
     5521 cgcgaacatc aaaatgatgg gcgtgggcaa ctataaagtg acctaccaca ttgaaccgcc
     5581 gtcaaaagcc gggatgcatc gccatactga cagcgaaaca ggcgtaggcc gctggtggaa
     5641 accgtttgat gtgagctacg agtttaaata tgtcggtctg aactaaaacc gactaaccaa
     5701 cgcgccagga ggattccccc ttctggcgcg ttggtcagac cgatcatatg cccgtcgctt
     5761 aattcaaccc gccgtacaga gtcgaagtta tgagttactt tttcgtcacg acgctacagg
     5821 tctttttctg cattgcgctt ctttccggtg ttctctggag tcgaaacgat cctccctctt
     5881 tacgtccgct gacctggacg ctgctgaccg ggctgattgc cggcgtgctg gcgggactgt
     5941 ttattcacgg cagccaaccg gtacaactgc tgctggttgg ggcggaggtc atggtatcgc
     6001 tgctgtttgt cctcagtttc tggtgggctt caacgcgtat ccgctatctg tggcagggga
     6061 ttctgatttt cggcgctgcc cgccattggg cgctggaccc taatctgggc gggctgacca
     6121 gtacgcatgt gctgaatact gacctgctgt taaacctgac ggccgtcgtt ctcgcttttg
     6181 cgatcctctg tctggcgggc gtactctgcg cgatgctgct gcggcgtatt cgcggcctgt
     6241 actggccgtt aacgctgatt ctgctggtga tgatctggct gccgctgagc ggcaacctgc
     6301 tgttgttgct gatgaaatta caggttgttc cgctgggaaa atcactgctg agcttcgtgg
     6361 cgaaagtcac aaataacacg gcgctgtata actgggcggg agccgcgctg ctgctggccc
     6421 tggcgctgtg ctggcttccg gcgctgttgc gggcttttcg acagacgcgg gaaaccgaag
     6481 aaccgattgc tcaccgtctg gcgctggcgc agcgtcgcaa cgctttgcgg ctgtggttgg
     6541 tcacaatcgg ctgcgcggtg gtcgttatcg caggccaact gtggtgggat aaagtggcct
     6601 cgcagccgcc gcaactctct gaagccgtgc ccgtaacgct ggggagcgat ggcatggtgc
     6661 gcctgccggt tgaacagctt cgtgacggta aactgcatcg tttcgtctgg gtggcggacg
     6721 atggcaaagc ggtgcgcttc ttcgtgatta accgctatcc cgacaagctg cgttttggcg
     6781 tggtgttcga cgcctgcctg ctgtgcggcg atcagggtta tgtcatggag ggcaatcagg
     6841 ttatctgcgt cgcctgcgga gtgcatattt ttattccgtc tatcggcaag gccggtggct
     6901 gtaacccggt gccgattgaa aactggcaca atgatgaaaa agagctggtg atccccggta
     6961 aagaactggc gacaggggtg aactacttct cgacggtcat gactattaaa gtgaccgatc
     7021 cggtcgatgg ttcaacgttg accaatacct cagccgatta caaatacagc tatggcggca
     7081 agacctggtt cttctcgtca gaggctaact atgaacgctt ccgtgaaacg ccggagcagt
     7141 ttgttcctgc cgacatgagg gaggaataat catgctgtgg cggatgttac gacaatcctg
     7201 ggggcgcaat ttacgccgca aagtgctggc gataatcacc gtttttctgg cctccagcct
     7261 gatctcggcg ctgctggcgg tgtccattga tatcggcgac aaaatgtcca gagagctgaa
     7321 gtcttatggt gcgaacattt tgattgaacc cgccggacag gctgcgctgc ccgcgctgtt
     7381 tagtgagagc agtaatccgc tttccggtca ggattttctt gatgaagccg agctgccgaa
     7441 catcaaagat atcttctggc gaaacaacat tgtcgggttt gcgccgatgc tcggcggcga
     7501 agccagcgtg gagggagaac cagtgcggat tctgggcact ttcttcagtc agccagtgga
     7561 tattcctgat gaagagggat atgagacggg gcagaaaacg gtcagcccat actggcaggt
     7621 gaccggcgac tggccgcagg aacccgcagg cgcagagccg cagaccttag tcgggcacgc
     7681 gctggcgcgg caaatgggct ggaaacccgg tgataagctg acattacgta ccgaaggcga
     7741 agcagtgcag gtcaccgtca gcggtatcct ctccagcggc ggcgatgaag ataaccaact
     7801 ggtgatgccg ctaagcacgg tacagcacct gctggggctc cccggaaaag tgcaggcgat
     7861 tcgcgtctcg gcgctgacgg tgccggaaaa cgaactgtcc cgccgcgcgc gggaaaacct
     7921 ggacgcgctc aatgccgaag agtacgacct ctggtattgc acggcctatg tgtcgtcgat
     7981 cgcgcatcag ctggaagagg cgatttccgg tgcggaagtg cgcccggtat ggcaggtcgc
     8041 ggcgtccgaa ggggtggtga tcgataaaat tcagttgctg atggcggtcg ttaccgtcgc
     8101 ggcgctggtg gcttccgcaa tggggatcgc ctcgctgatg accagcacca ttatggaacg
     8161 cgccaaagag atcggtttga tgaaagcgct gggtgcgcga cagtggcaaa ttatgctgct
     8221 gttctacctg gaagcggcgt ccagcgggct ggctggcggt gcgctgggat gtattgccgg
     8281 gtgggggctg gcgaaagcga ttggcgtcat gctcttcgac gcgccgctta attttgcctg
     8341 gattgtggtg ccgtgcgtac tggtgattgc ggtactgatt gcgttgatcg gcacctggtt
     8401 cccggcccgg cggattgccc ggctgtatcc tgtggaggtg ctgtatggcc gctaaacgta
     8461 ccatgctctg gctgctggtg tggcgggcgc tgcgtctgcg ttttcagcgg gtcagcgtgg
     8521 tgtttgccgc gctgatggtg ggggcgacca tcgtgacggc gctttttgcc gtctggttcg
     8581 acattaacac caaaatgagc gaagaactgc gcaccttcgg cgcgaatttt tacatcgggc
     8641 cggggcacgg ttccagtatg ccgcagcagg agttgcagag cattctcgat caggcgccgc
     8701 aggggctggt gcatggcgcc agtccgtggt tgtacggcat ggcgcgtacc gagctggaaa
     8761 aagtggtgat ggttggcgtg tggttcgagt cgctgcaaaa actggtgccg tactggcagg
     8821 tcaccggtag ctggattggc gttagttttg acgaccgtaa tgcgatgatc ggcgtcaaac
     8881 tggcggaacg cctcaacgtg cagccgggcg acagcatcac gctggtggat cacaatcagc
     8941 gtaagaattt gcagattaaa ggcattgtgg agtctggcga tgcgacggac aacatgctga
     9001 tcgtcagcct cgacgtggcg caggcctggc tgcatcagcc gggcaaaatc agccacggct
     9061 tgctgagcgt cagtaacgat gtcggccagg tggagaacta tgccagccgg ttgcaggcgc
     9121 agtatcctga cctggagatt cgtccggtgc gtaaggtctc tgcttccgaa gggcaggtgc
     9181 tggataaaat taaaggtctg atgggactgg tctcgatcgt gattctggcg ctctcttcgc
     9241 tgtgcgtgaa taccacgctg atggcgattg tcggcgagcg ggcgcgcgag tttgcgctgc
     9301 aaaaagcgct tggttccagc aacggcgata tcgtgcggca aattctgctg gaaaccagca
     9361 tcatcgcgct ggcggcggtg gcgtgcggct gggtgctggg atatttgctg gcgcaactgt
     9421 tgggattaac ggtgtttaat gccgccattt cactgcgtct gccggtgttg cccattactc
     9481 tggtgttgtc tttactggtc gccattctgg cggcgattgt tccggtgcga cgcgcggtaa
     9541 gcgtcgagcc tgctaaagtg ctgaaaggag agtagcgcca tgtccgttac gcatatccct
     9601 caggtggcga tcgaaacccg ccacttatac aaacggttcg gtgacgttac cgccctggat
     9661 gacattaatc tgcgcattgc gcagggtgaa tttgtcgcca ttatgggcgc ttctggttct
     9721 ggtaaaacca cgctgatgaa tattctgacg tgccttgata cggcgacgga agggcaggtg
     9781 tttcttgatg gcaccgatgc cgccgcgctg gatgaagagg ggcgccgtcg ctttcgcgcc
     9841 gagaaaatcg gcctggtatt tcagcagttc catttgatcc cgttcttaac tgcgctggaa
     9901 aacatcatgc tcgcccagca ttaccacagc gtggtggatg aagcggcggc gaggaaggtg
     9961 ctggaacagg tagggcttgg gcatcgtgtg acgcacctgc cgagccagct ttccggtggt
    10021 gagcagcagc gtgtgtgtat cgcccgggcg ctggtcaacg aaccgccggt tattttcgcg
    10081 gatgaaccga cgggcaacct ggatgaagag aatgagcagc gggtgctgga tctgctcacc
    10141 gatctgcatc gtcaggggcg cacgattgtg atggtgacgc acaaccctgc gcttgggcag
    10201 tttgccgacc gtattctgcg gctgcaacac ggtaaatatc tgggtgagga ggccaatcaa
    10261 catgcgctgg cataatgtgc tgttactcgc ggcggcgatc ctgataagcg ggtgtaaaga
    10321 agaaaagctg gcggtcgggg caccggcccc ggcgctggcg gcgtttgatc tgcaaggcca
    10381 ggagtcaggc cttgaacgct ggcaaggaaa ggcggtgtac ctgaattttt ggtctgcggg
    10441 atgcgggggg tgcctagcgg aaatggacac gctggaagcg ctgagcaaaa aatggggcga
    10501 taaagtggtg gttgtggcgg tcaataccga ccctgaaacg gtcaggatca acgatctgct
    10561 ggcaaaacat cagataacct atccggtggt gcgcgatcag ctcaacatta cccaggaacg
    10621 ttatcaggtg atcggcacgc cgacctccgt actgattgac tccgaaggac gcgttctgga
    10681 gctacaccag ggtatgcgta agccgtctga gttagaggcc acgtttgcgc gcctggcggc
    10741 gcgatgaacg acgcgtacgg agagataccc aatggcattt atctggaacg acgaaagcct
    10801 tgcgattctg cgggaaaacg cgggcatcct cacgacagag cagatagcgc aactgcttca
    10861 cactaatatt accgccgtgc ggaatatggc ctatcggctg aaactgagcc ttcgggtgac
    10921 ggcgtataat caccgacgca tagcgcaggt tcaggcgctt tatgcctctg agacgttgag
    10981 tctcaaagag atcgcggcga aaacggggct gacggccagt accgtgcaat atatcgtgta
    11041 cgtgaaatca aaaaataagc cctacgccac cacggaatat gtctcttttg aaacggagaa
    11101 cgccgttcat taccgcgtgc aaaaagaatt tgtcgatacg gaacgttccc tgctggacaa
    11161 catctctgat aacacgcgat ttcgtgaact gtatctgacg gacggcactt tttattgcgc
    11221 ccgcaatatt aagtatgagg tttttatctc ggagtaaccg gaacacgctg acctgccggt
    11281 tatcttctgg aatctcccgt tttgcgaaac cgttcccgga aaaatgtcat atttctgtca
    11341 cactctatga ggattgataa caacaagagg ttgtagggat gaaaaaattg atactcgccg
    11401 ccgctgtggc aggggctgtt ttattgtcgt ccgccgcgca agcgcagaca acgccggaag
    11461 gttatcaact gcaacaagtg ttgatgatga gccgacacaa cttgcgcgcg ccgttggcca
    11521 ataatggcag cgtgctggag cagtccacgc cgaatcagtg gccggagtgg gatgtgcccg
    11581 gcgggcagct caccacgaaa ggcggtgtgc tggaaattta tatgggccac tacatgcgag
    11641 aatggctggc ggagctgggg atggtcacgt caggcgaatg cccgacgccg gacacggtat
    11701 atacctatgc caacagtctg caacgcaccg ttgcgacggc acaattcttt atcaccggcg
    11761 ctttccctgg gtgcgacatt ccggtacatc atcaggaaaa aaatgggcac gatggaccca
    11821 acgtttaatc cggtgatcac cgatgactct gcggcgttta gccagaaagc ggtgcaggcg
    11881 atggaaaaag agcgcagcca gatgcagctt gacgacagct atcagctgct ggcgcagatg
    11941 acggactaca aagattcacc gtcctgcaaa gaaaaacagc agtgttcgct gaccgaagcc
    12001 aaagacgcct ttagggaagg tgcgaataag caggtcatgt cttcccaagc tgactcgttg
    12061 attaaaattt cgcggatctg ggccgatttt tgtcccgcaa acacatcgaa tcagcctatt
    12121 taggctattt tttccaccat ttctggtgtt atttccggtt tttactgaga tctctcccac
    12181 tgacgtatca tttggtccac ccgaaacagg ttggccaggg tgaataacat cgccagttgg
    12241 ttatcgtttt tcagcagccc cttgtatctg gctttcacga agccgaactg ccgcttgatg
    12301 atgcgaaacg ggtgctccac cttggcacgg atgct
//
LOCUS       DAZFRE010000127        12292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFRE010000127 DAZFRE010000000
VERSION     DAZFRE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.127"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..632
                     /locus_tag="ACHKUI_004345"
     CDS             <1..632
                     /locus_tag="ACHKUI_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6600416.1"
                     /translation="YRIMRQNALLLERKTAVPPSKRAHTGRVAVKESNQRWCSDGFEF
                     RCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIIPKPD
                     GLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
     gene            complement(745..1938)
                     /locus_tag="ACHKUI_004346"
     CDS             complement(745..1938)
                     /locus_tag="ACHKUI_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600417.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2074..3797
                     /gene="iucA"
                     /locus_tag="ACHKUI_004347"
                     /pseudo
     CDS             2074..3797
                     /gene="iucA"
                     /locus_tag="ACHKUI_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHKUI_004348"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHKUI_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO6600418.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHKUI_004349"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHKUI_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO6600419.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6484..7817
                     /gene="iucD"
                     /locus_tag="ACHKUI_004350"
                     /pseudo
     CDS             6484..7817
                     /gene="iucD"
                     /locus_tag="ACHKUI_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            7843..10044
                     /gene="iutA"
                     /locus_tag="ACHKUI_004351"
     CDS             7843..10044
                     /gene="iutA"
                     /locus_tag="ACHKUI_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO6600420.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10595..10738)
                     /locus_tag="ACHKUI_004352"
     CDS             complement(10595..10738)
                     /locus_tag="ACHKUI_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600421.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
     gene            10988..>12292
                     /gene="sat"
                     /locus_tag="ACHKUI_004353"
     CDS             10988..>12292
                     /gene="sat"
                     /locus_tag="ACHKUI_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HFO6600422.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVIL"
BASE COUNT         3120 a         3074 c         3186 g         2912 t
ORIGIN      
        1 tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaaaccgct gtaccgccat
       61 cgaaacgggc acataccggc agagtggctg tgaaagaaag taatcagcga tggtgctctg
      121 acgggttcga gttccgctgt gataacggag aaaaactgcg ggtcacgttc gcgctggact
      181 gctgtgaccg tgaggcactg cactgggcgg tcactacggg cggcttcaac agtgaaacag
      241 tacaggacgt catgctggga gcggtggaac gccgcttcgg caacgagctt ccggcgtctc
      301 cagtagagtg gctgacggat aatggttcat gctaccgggc taatgaaaca cggcagtttg
      361 cccggatgtt ggggcttgaa ccgaaaaaca cggcggtgcg gagtccggag agtaacggca
      421 tagcagagag cttcgtgaaa acgataaagc gtgactacat aagtatcata cccaaaccag
      481 acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc
      541 cgcatagtgc gttgggttat cgctcgccac gggaatatct gcggcagcgg gcctgtaatg
      601 ggttaagtga taacagatgt ctggaaatat aggggcaaat ccaggcgggg gcagcaagcc
      661 agaacactgc ctgacatgtt ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtggcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccagca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata acatttatta ttgataatga gaatcattat tgacataatt
     2041 gttattattt tgctgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc
     2101 acagatgtgg ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa
     2161 ctgacagaat acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat
     2221 ttcagagtgg cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg
     2281 gtcacggcat caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat
     2341 aagctacgcc atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg
     2401 ctggaaagcc atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg
     2461 cgtgaaaaag cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc
     2521 caccctgcgc ctaagtctca tgaaccgttt aaccggcggg aggctgaacg ctacctgcct
     2581 gatatggcac cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt
     2641 gaaagtttgc atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct
     2701 cagttactca acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg
     2761 gaatatcttt tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac
     2821 ttaggtgagg ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc
     2881 accagccgcg atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact
     2941 ctgtccgtga aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc
     3001 tggcagatgc tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc
     3061 gggctgcgcg atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat
     3121 ctgctggtgg atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca
     3181 cctgatggcg gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt
     3241 atcactgtgc aacaggccgc ccatgcatgg gtcgatgcat actgtcagca agtgctaaag
     3301 ccgctgttta cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt
     3361 cttgtccaga tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc
     3421 gcttttatgc ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat
     3481 attttcaccc atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact
     3541 tttgctgtga ccgctgcgct ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgctccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 tgcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cggactggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgctaccgac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagtc gccggggcgg gctggatatc aagctgccgc tgaccatcta
     5581 caacacctca tgctaccggg ggatccctgg cagatacatc gctgccggac cactggcttc
     5641 acgctggcta caacaggttt ttgcgaccga cgccacccta gtgcaaagcg gcgcagtgat
     5701 acttggtgaa ccggctgcag gctatgtctc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtccgg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tagaccgctc cgggctggac gctgaaacct ggcttacgca
     5941 actgttccgg gtggtcgtgg ttcctctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacat ggacaaaata taactctcgc catgaaagag ggggttccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcgact ggtgaaagaa gagttccccg aaatggactc
     6121 tttgcctcag gaggttcgtg atgttacatc ccgcctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg taacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 aaaacatcca caaatgtcag agcgttttgc gcttttctca cttttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggcctgat ctggatggcg gcagccgcat
     6421 gctgccgaat taccttgagg atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtagggacag ggccatttaa tctcagcatt
     6541 gctgcgttgt cacatcagat cgaagaactg gactgtctct tctttgatga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgctgttgc acctacaaat ccctacagtt ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc agactacgta cagtatcccg tgaagagttt
     6781 tctgactacc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaacattg atttcgataa aaaacgtcga ttgtttctgg tgcaaaccag ccagggagaa
     6901 tattttgccc gcaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatatga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctt
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcccg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactggagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gccgctcggt aacaactctg
     7381 gaaagtagtg gtcctggctg gaagttgctg atggagcatc atctggatca gggcagggag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcgtt gccacaaata
     7501 cttccctcac tgatgcccct gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gagaacaaca tcttcgtggt caacgccagt
     7621 atgcaaaccc atggcatcgc cgaaccccag ctcagcctga tggcatggag atctgcacgt
     7681 attcttaatc gcgtaatggg acgtgattta ttcgatctca gtatgccgcc cgccctgatt
     7741 cagtggcgca gcggcaccta gggaaaacgc agccggaggc tgcttcttta actcgctaca
     7801 cagcatcttt gggctgattt tttccgcccg tatggaggaa taatgatgat aagcaaaaag
     7861 tatacgcttt gggctctcaa cccactgctt cttaccatga tggcgccagc agtcgctcaa
     7921 caaaccgatg atgaaacgtt cgtggtgtct gccaaccgca gcaatcgcac cgtagcggag
     7981 atggcgcaaa ccacctgggt tatcgaaaac gccgaactgg aacagcagat tcagggcggc
     8041 aaagagctta aagacgcact ggctcagctg atccctggcc ttgacgtcag cagccggagc
     8101 cgcaccaact acggtatgaa tgtgcgtggc cgcccgctgg tcgtgctggt tgacggcgtg
     8161 cgtctcaact cttcacgtac cgacagccga caactggact ctatagatcc ttttaatatc
     8221 gaccatattg aagtgatctc cggtgcgacg tccctgtacg gcggcggcag taccggtggc
     8281 ctgatcaaca tcgtgaccaa aaaaggccag ccggaaacca tgatggagtt tgaggctggc
     8341 accaaaagtg gctttagcag cagtaaagat cacgatgaac gcattgccgg agctgtctcc
     8401 ggcggaaatg agcatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg
     8461 tttgacggta acggcgatgc caccttgctt gataacaccc agaccggcct gcagtactcc
     8521 gatcggctgg acatcatggg aactggtacg ctgaacatcg atgaatcccg gcagcttcag
     8581 ttgatcacac agtactataa aagccagggc gacgacgatt acgggcttaa tctcgggaaa
     8641 ggcttctctg ccatcagagg gaccagcacg ccattcgtca gtaacgggct gaattccgac
     8701 cgtattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag cgcttttctg
     8761 ggacaggagc tggtcggtca ggtttactac cgcgatgagt cgttgcgatt ctacccgttc
     8821 ccgacggtaa atgcgaacaa acaggtgacg gctttctctt cgtcacagca ggacaccgac
     8881 cagtacggca tgaaactgac tctgaacagc aaaccgatgg acggctggca aatcacctgg
     8941 gggctggatg ctgatcatga gcgctttacc tccaaccaga tgttcttcga cctggctcag
     9001 gcaagcgctt ccggagggct gaacaacaag aagatttaca ccaccgggcg ctatccgtcg
     9061 tatgacatca ccaacctggc ggccttcctg caatcagact atgacatcaa taatctcttt
     9121 accctcaacg gtggcgtacg ctatcagtac actgaaaaca agattgatga tttcatcggc
     9181 tacgcgcagc aacggcagat tgccgccggg aaggctacat ccgccgacgc cattcctggc
     9241 ggctcagtcg attacgacaa cttcctgttc aacgccggtc tgctgatgca catcaccgaa
     9301 cgccagcagg catggctcaa cttctcccag ggcgtggagc tgccggaccc gggtaaatac
     9361 tatggtcgcg gcatctatgg tgctgcagtg aacggccatc ttcctctaac aaagagtgtg
     9421 aacgtcagcg acagcaagct ggaaggcgtg aaagtcgatt cttatgagct gggctggcgc
     9481 tttactggca ataatctgcg cacccaaatc gcggcctact attcgatttc tgataagagc
     9541 gtggtggcga ataaagatct gaccatcagc gtggtggacg acaaacgccg tatttacggc
     9601 gtggaaggtg cggtggacta cctgattcct gatactgact ggagtaccgg agtgaacttc
     9661 aacgtgctga aaactgagtc gaaagtgaac ggtacctggc agaaatacga tgtgaagaca
     9721 gcaagcccat caaaagcgac agcctacatt ggctgggcac cggacccgtg gagtctgcgc
     9781 gtgcagagca ccacctcctt tgacgtgagc gacgcgcagg gctacaaggt cgatggctat
     9841 accaccgtgg atctgctcgg cagttatcag cttccggtgg gtacactcag cttcagcatt
     9901 gaaaacctct tcgaccgtga ctacaccact gtctgggggc agcgtgcacc actgtactac
     9961 agcccgggtt acggcccagc gtcactgtac gactacaaag gcaggggccg aacctttggt
    10021 ctgaactact ctgtgctgtt ctgacgggga tgcttttgca acaagggtat gctgtaaaca
    10081 acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct taacaagata
    10141 ttccgtctcc ggaatggact gttcaccact gaataacatt atgtctgttt tatggatctt
    10201 tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa ccttacaagt
    10261 aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg atctgcgccc
    10321 aatatcctga gccggaactt tgctgcaatg aaggccaacg aaaaatgggg gagtatgtta
    10381 tagagtactt gctacagggt aaaaagaggt acctgtcgct ggttctccgt tcagtttaac
    10441 cgggagattt tctcctgcgc ctgtcataaa gtccggtaat ggagattgtt aatacgatgg
    10501 tgcacgatgc attgtcagtg cccggcccgg aggatgcccc gtagctacat acggatcagg
    10561 gttggcaata tcgaatggca ggctataggc aaagttaaag ccccatggag tagcacaaaa
    10621 tatgccgcac aaaggaaacg gtctgaataa cgcagcgatg gagaacttct tcggcacact
    10681 gcaaaatcgc atcgtggtcg atcgctaatt cgtcgtgaat cactgaacga tgttccatgc
    10741 gaacagatat tcgatcatat cgaaatatta aacattaccc ctccccacat tgggcaaaaa
    10801 aaaacaaaaa acgatcaatt aacgatcaat tagtattgat tcacataatc tctcatcatt
    10861 aatgagaaca ttgtcacata tttacaatat agaactgtta cttttagcaa gctgttagta
    10921 accaaccgtt actaataatt ttaattctta atactttgtt cattcaataa atgagttgag
    10981 agaatatatg aataaaatat actcccttaa atatagtgct gccactggcg gactcattgc
    11041 tgtttctgaa ttagcgaaaa gagtttctgg taaaacaaac cgaaaacttg tagcaacaat
    11101 gttgtctctg gctgttgccg gtacagtaaa tgcagcaaat attgatatat caaatgtatg
    11161 ggcgagagac tatcttgatc ttgcacaaaa taaaggtatt ttccagcccg gagcaacaga
    11221 cgtaacaatc actttaaaaa acggagataa attctctttc cataatctct caattccgga
    11281 tttttctggt gcagcagcga gtggcgcagc taccgcaata ggaggttctt atagtgttac
    11341 tgttgcacat aacaaaaaga accctcaggc cgcagaaact caggtttacg ctcagtcttc
    11401 ttacaaggtt gttgacagaa gaaattccaa tgattttgag attcagaggt taaataaatt
    11461 tgttgtggaa acagtaggtg ccaccccggc agagaccaac cctacaacat attctgatgc
    11521 attagaacgc tacggtatag tcacttctga cggttcaaaa aaaatcatag gttttcgtgc
    11581 tggctctgga ggaacatcat ttattaatgg tgaatccaaa atctcaacaa attcagcata
    11641 tagccatgat ctgttaagtg ctagtctatt tgaggtcacc caatgggact catacggcat
    11701 gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag acagcggctc
    11761 tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa caacccatgg
    11821 tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg ataaactggt
    11881 tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata atgtaaccat
    11941 aaaaaacaca gatataacat tacaccaaaa caatgcagat accactggta ctcaagaaaa
    12001 aataactaaa gacaaagata ttgtgttcac aaatggggga aatgtcctgt ttaaggataa
    12061 tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata acataaacgg
    12121 tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca ttgtaaactg
    12181 gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta ctctgaatgt
    12241 tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc tt
//
LOCUS       DAZFRE010000128        12207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFRE010000128 DAZFRE010000000
VERSION     DAZFRE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.128"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(488..1060)
                     /gene="hdeD"
                     /locus_tag="ACHKUI_004354"
     CDS             complement(488..1060)
                     /gene="hdeD"
                     /locus_tag="ACHKUI_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6600423.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            1315..1647
                     /gene="hdeA"
                     /locus_tag="ACHKUI_004355"
     CDS             1315..1647
                     /gene="hdeA"
                     /locus_tag="ACHKUI_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6600424.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            1763..2089
                     /gene="hdeB"
                     /locus_tag="ACHKUI_004356"
     CDS             1763..2089
                     /gene="hdeB"
                     /locus_tag="ACHKUI_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6600425.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2153..2800
                     /locus_tag="ACHKUI_004357"
     CDS             2153..2800
                     /locus_tag="ACHKUI_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6600426.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2852..3622)
                     /locus_tag="ACHKUI_004358"
     CDS             complement(2852..3622)
                     /locus_tag="ACHKUI_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO6600427.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3619..4611)
                     /gene="shuU"
                     /locus_tag="ACHKUI_004359"
                     /gene_synonym="chuU"
     CDS             complement(3619..4611)
                     /gene="shuU"
                     /locus_tag="ACHKUI_004359"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO6600428.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4660..5283)
                     /gene="chuY"
                     /locus_tag="ACHKUI_004360"
     CDS             complement(4660..5283)
                     /gene="chuY"
                     /locus_tag="ACHKUI_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO6600429.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5283..5777)
                     /gene="hutX"
                     /locus_tag="ACHKUI_004361"
     CDS             complement(5283..5777)
                     /gene="hutX"
                     /locus_tag="ACHKUI_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO6600430.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5790..7127)
                     /gene="hutW"
                     /locus_tag="ACHKUI_004362"
     CDS             complement(5790..7127)
                     /gene="hutW"
                     /locus_tag="ACHKUI_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO6600431.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEREAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(7147..8061)
                     /gene="chuT"
                     /locus_tag="ACHKUI_004363"
     CDS             complement(7147..8061)
                     /gene="chuT"
                     /locus_tag="ACHKUI_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HFO6600432.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8745..10727
                     /gene="chuA"
                     /locus_tag="ACHKUI_004364"
                     /gene_synonym="shuA"
     CDS             8745..10727
                     /gene="chuA"
                     /locus_tag="ACHKUI_004364"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO6600433.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWLSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10776..11804
                     /gene="chuS"
                     /locus_tag="ACHKUI_004365"
     CDS             10776..11804
                     /gene="chuS"
                     /locus_tag="ACHKUI_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO6600434.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(11861..>12207)
                     /locus_tag="ACHKUI_004366"
     CDS             complement(11861..>12207)
                     /locus_tag="ACHKUI_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6600435.1"
                     /translation="RLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALINGSY
                     CSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRIN
                     ELVRHQHINYLV"
BASE COUNT         3240 a         2875 c         2998 g         3094 t
ORIGIN      
        1 gtaatccgat ttaaatatcg agtctccttg tttcgactta ggctggcaat tggattgcca
       61 gctttctttt aacgcattga aattacctga ttaaataact cattcatgtc ttcaggtttt
      121 tatatagcgt ctggagacac ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa
      181 tgaacccgga ttacttattt aaccgcccat agcatcaacg cttacggaac tctttgttca
      241 ctttcccaca gcacttttaa aacattaaac ctgaccacgg aaaatatcag ccatgataat
      301 gtttgaaatg gctaatttgc catggaacga aaaaaatcag attaaattca gtaggttgaa
      361 ataatcaaca gcaggcaatt gtttcaatga tagtgcgtaa ttgaactgaa acactgtata
      421 gctaagagag tattaactgg atcatagtca cttaatgtga ctatgatcca ggtggaaata
      481 gggataatta ttgcttttta acgaacaaac tggcgaagct gaacagactg gcggcgctaa
      541 atatcagttc aattcccacc agtgtggaaa ccagcgttac cgacaccatc ggcgtagcac
      601 caaggaatat ccaggcaatg acgatatcca gcacaccaat aacgagctgt agccaactgc
      661 ctttcattga acgctgacga taccaactca tcaggcgaat aacccctgcc acacagaaca
      721 aaccggcaat aaatgccgca atggcaaaaa tgcccagctc tggtgcgcgg atgaagaaat
      781 agccgatcaa taaataggcg actgcgacga ggaaaccgga taataccggc cagaaattat
      841 gactgcggtt gctgaatagc ccgacaataa gcgcaatgcc cgagcagatt aataatgcac
      901 ccactactgt gcttaaaatg tcgccagaga cgaacggaaa actgatacac agcaacccga
      961 cgataaacag cagcacggca ataaactgga ttgctctgcg atgtttttta agcatctcca
     1021 gatcaaactt caaaattgtt gccttatcta tatataacat agaaccaccc tataaaatta
     1081 agaagaaaat cccctgctat caatctatgc caaaaacgcg tgtaagaatg cagtcgattt
     1141 aataaaaatt tcctaattac agtatctgat gcgtctgtaa ctcattgtat tgaaataaaa
     1201 atatctgatt ttgatatttt ccatcaacat gacatataca gaaaaccagg ttataacctc
     1261 agtgtcgaaa ttgattcgtg acggctcttt cactttatag ttgaggatat tacgatgaaa
     1321 aaagtattag gcgttattct tggtggtctg cttcttctgc cagtagtgag caatgcagcg
     1381 gatgcacaaa aagcagctga taacaaaaaa ccggtcaact cctggacctg tgaagatttc
     1441 ctggctgtgg acgaatcctt ccagccaact gcagttggtt ttgctgaagc actgaacaac
     1501 aaagataaac cagaagatgc ggttttagat gttcagggta ttgcaaccgt aaccccagct
     1561 atcgttcagg cttgtactca ggataaaaaa gccagcttta aagataaagt taaaggcgaa
     1621 tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg ttacatcgta tttccttgca
     1681 tattgaacag gccggaatat cttctttaaa agcagctatt cctcctgttt gtatataatc
     1741 tctatattga atgggttaca aaatgaacat ttcatctctc cgtaaagcgt ttatttttat
     1801 gggcgctgta gcggctttgt cactggtgaa cgcacaatct gcgctggcag ccaatgaatc
     1861 cgctaaagat atgacctgcc aggaatttat tgatctgaat ccaaaagcaa tgacaccagt
     1921 tgcgtggtgg atgctgcatg aagaaacagt atataaaggt ggcgataccg ttactttaaa
     1981 tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac tgcaagaaaa acccgcagaa
     2041 aaatttgtat accttcaaaa atcaagcatc taatgacttg ccgaattaat aaggtacaag
     2101 taaaaaggag tagcaagttg agccatcttg ctgctccttt ttgcattttt atatgacagc
     2161 agaatttatt atacgactta tacttgcggt aattgcctgt ggcgctattg gcatggaaag
     2221 gcaaatgcgc ggcaaaggag cagggttacg cacacatgta ttaattggca tgggaagcgc
     2281 cctgtttatg attgtttcga aatatggttt tgctgacgtg ctgtctttag gtcacgtcgg
     2341 actggatccc agccgtattg ctgctcaggt tgtgacgggc gtcgggttta tcggtgcagg
     2401 taacattctc gttcgtaacc aaaatattgt aggtctgacg acggcagcgg atatctgggt
     2461 gaccgccgcc attggtatgg ttattggcag cggtatgtac gaactgggta tttatggttc
     2521 agtgatgacc ttgctggtgc tggaagtctt ccatcaatta accttccgcc tgatgaacaa
     2581 aaattaccat ttacagctga cattagtgaa cggtaatacg gtatcaatgc tcgactggtt
     2641 taagcagcaa aaaataaaaa cggatttagt ttcgttgcag gaaaatgaag atcatgaagt
     2701 ggtcgccatt gatatccagc ttcatgccac gacgtcgata gaagatctgt tgcgattatt
     2761 aaaaggtatg gcaggggtga aaggcgtttc aattagctaa tctgtatagt tatttattcg
     2821 caggagtacc agtgcggtac tcctgttggt tttaatgatc gaggacgatc agtggtgaat
     2881 ggttggcagg atctttaagt acggtaatat ccgcgccgta gagcattgtc agcgcctgct
     2941 gtgtcaatac gtcctgcggc ttaccatttg cgataacctt tcctttttgc attaatacaa
     3001 cacggtctgc ataacgcgcg gcaagattta gatcgtgcaa tacgcagcaa acattgaact
     3061 gccgttcgtg aactaactgg cgtaataaac gaaacaagtg ctgctgatgg tgaatatcca
     3121 gcgccgaagt tggttcatca agaaataacc atttcggtga gggagtaggt tcccagagtt
     3181 gcactagcag gcgcgctaat tgcacgcgct gttgttcacc accggataat tgccgatagt
     3241 cgcggtttgc taacgcctga cagtcacata acgccattat ttgtgctgtt tcattatcct
     3301 gattaccagt acgatgcgga tgccgcccca tctggataac ttcttgtacg ctgaatggaa
     3361 acgccatgtg gctattttga cgcatcacgg cccggtgttt cgccagttca gtaatcgacc
     3421 attcatttaa tggcttatta aacaaccgac attcgccgct atcaggttga agatagccgg
     3481 ttagctggcg aagcagcgta gattttcccg caccattagg cccgaggatg gcaacaattt
     3541 ccccaccggg aaaagtcagc gagacattat cagtcagacg ccgcccctga aggctataaa
     3601 ccagattctg ggcgctgatc atgtgatcct ccggttgcgc agaatcaacc acataaaata
     3661 agggccacca agcaggctgg ttaataatcc cacgggcatt tctgctggct gtaccagcgt
     3721 gcgggcaagc gtgtctgcca tcagcaataa acaggctccg gcgagggcgg aacaagggat
     3781 tagccagcgg tgatttgccc cggtagtcat gcgaatcaga tgcgggatca ccaggccaat
     3841 aaagccgata atgccgctta ccgatacggc agcaccaacg agcagtgagc tcactaacag
     3901 taattgctgg cgtttacgct tcacgttcac gccgaggtag tgggcttctt catcaccgag
     3961 ctgcagtaaa ttcagcgttc cggcgagaca agttgttgcg ataatggccg gtaggatgaa
     4021 tgaactggca accaataacg tcggccattg cgcctgtcca agattgccca tcatccacaa
     4081 tgtgagttgt cgtagctgct gctcatcgcc gatatagctc aggatgccga tcgctgcgcc
     4141 gcacagggca ttgatggcga taccggcgag taataattgc atcatgctgc catcgcgatg
     4201 gcgctgcgtg atgagaaaaa tgatggtgca gaccactaaa cttccggcaa tggcaaacac
     4261 catttgctca taaagcacca gcaccactgg gaaggagaag ggcagcacaa tagcaacgcc
     4321 aaccattaat gcagaaccgc tactgacgcc aagtaatcca gggtcagcca ttgggttgcg
     4381 aaaaagccct tgcataatta cccctgcggt tgccaacgct gcacctacca gcaccgccag
     4441 caaaaccctt ggtagacgaa tattgagcca gatatcgcgg tattcctcat caaaaagggc
     4501 atcaaaactg atctttaacg ctccctgact ggcaccaaac agcaccagag ctagcaataa
     4561 tagtgatagc cccataaaga ccctggcgga agagagtgaa tccttgagca cgcaaaactc
     4621 cgttacgaca ttacggcgcg gacacgccgc gccgggggat cacgccggtt tcaggtccgg
     4681 ttcaatcaga ctgtagacct gctggttaag tgctggcgca tttgccagtt catggatatg
     4741 tgcggcgaca tcagcacggt taacaaaacc atgacactcc tgattttgta tccgttgtgc
     4801 tttgcccgtt gccgcaccat caagcagacc gccggggcgg agaatggcat aatcaagctg
     4861 gctggtttgc aaccagcttt ccgccagcgt tttttcccgt accgcctgac caaacgcagc
     4921 ctttgcccgc tctgataaaa acggccagct atcgccgcat cccaaagagg tcaccagaat
     4981 catgcgactg atgccagcct tctcggcctc atcaatcact gttctgtgcg ccagataatc
     5041 ctgagctccg cccattgttg agatgataag cgcatccggg ccagctgcgc ggcaagcagc
     5101 cgcgactacg ctggcatcac aggcgtcgcc tgtaaaaacc tgtacgcctt gttgcgccag
     5161 tttcgtggcg gcatcggcat gacgaatgac agcaacgacc ggacgttgtt ccgccagcgc
     5221 cagttcaagc gttctggcac ccacaccttt tccgcctgcg ccaaagagta gccacggtgt
     5281 catcaggcgt gttcctttaa cgatgctgcc agagcatgga aggcgctgac ttgttcgctc
     5341 aggagctgac gatgatcatc cctaccgagg aagattttca gcattgcgct gccttcctta
     5401 ttgaaaaaga gaatagaggc ggtgtccatg cccataaatt tacgttcgat caaggcaata
     5461 tgcgtgcagt tttctgcttt gatatgcccg gacatgccgt gctttccgcg taggttgaaa
     5521 tagccgtggc gatggaaacc cgaaggcagt tcgccgctaa attcgagtat tacatcggta
     5581 gtatgtacta acgtggtgac gttgccccat tcgcagacgg tatcccagac agtatcgaat
     5641 ttatcacctg acacaacagt cggagagggc agatttttca ctacctccag cagcgtggtg
     5701 ttgtattgtt cggcgatcgc ctccagcgta ccgtctggct ccgttttcaa aaattcctgt
     5761 aacgagacat ggctcataaa tcactcctgt tatggttttt caagcgcaat tgctggcgca
     5821 ttaagtacct gaatcagttc attaagagac tgcaaaatgt tgcttgccca gaaacgacct
     5881 tcattggtca accgcagaca cgttgacgca tcgcggctta agcctttttg gtgccactgg
     5941 gccagtaacg gcgcgagttt ttcggcatgt ggcgtaagtt cgtccagcgg tacacgcgcc
     6001 gtttcaatgc ccgactgcaa ggtatgacgc cactgcgcgt tacgttcggc gttacgcatg
     6061 atcatcatca gcggtttttt gcctgctgcg acggattcat gccaggtctg aagattgcgt
     6121 tcgttcatcc aggagtaccc attaatcgaa ccgccggctc cggaaccaaa ggccagacaa
     6181 tcggcacctt gtttaatcag caggttatag agattgcgtt cgcgtgtggt acggccccag
     6241 tggctgttac tgatgcagcg ccaaccggca tcgtccataa aatcacaccc ttgcaggtaa
     6301 agatcgcgac gttctgccgg agagggcacg gtagtacgcc cattttccac ggctttgccc
     6361 agcggtgtat tgggcaggac attgagcgca tagagatcga cgccgtcgag accgatatcg
     6421 cgggcaatag ccagatcttc tccccaggtt tgcgcatcct gacccggcag accaaacagc
     6481 aggtcacaga ccaccgcggc acggtcgcgt ttgaccaggc tttccataaa cgcaatagcg
     6541 gttgggccat ccgaggtgcg ggccattttc ttgcggattt tgctgttaaa cgactgaata
     6601 ccaattgaga agcggtttgc tccagcatca aggcaagcat cgattcgctc agcgtcaaaa
     6661 ttcagtaccc ggccttcgat agtgatttca caatcagggg ccagtggcag cttttctcgt
     6721 aatgtggtga tgattctggc caaatcgtgt gccgaaagcg cggaaggcgt accgccaccg
     6781 aaatagaccg cgtggatggg ggccgactga tgcaatacgc tgtctgcttc ccgttctatt
     6841 tcacgaatca gggcgtcggt ataatgagca cacgcatctt cattaaaacg gttctgataa
     6901 aaaccacaaa acgtgcagtg tgtcgcacaa aaaggaatgt gtaggtaaac cagtcgctta
     6961 cgtggtgata ccgtttgatt gatcatctct tgccaggtct gtgccagttg ctctttggca
     7021 accggaatgg ccccacgaaa cggcatcatg gcgcgcctgt ctttaaaggg ctgatcaccg
     7081 tctaaagcaa aatgtggcgt gagatccagg gtgttatttg tgttcattcg tctgatttaa
     7141 atccatttat tgcggccaaa gctgctggtg taaagactca acgacatcag caatacgcgg
     7201 acccattccc agaatcaggt tttgatcaac ggtgataatg cgctggtttt tccatgcggc
     7261 agtgtgggta attccggcaa tagaacgtag ccgattaata tcgccatcga ccatttgtga
     7321 ggtgacgaca atcacttcag ggttagccgc aatcagcgat tccgcgctgt agcttttgta
     7381 ctgctggtgg gtagcgacgt tttctgctcc ggcaagcgaa agaatggcgt ccgcgacgct
     7441 gcctttaccg gcaacctgtg gcgcacttcc gcctgcggag agaataaaca tggctttaac
     7501 cggggctttt ttggccgcca cgttttgctg tactcgctcc aggcgttggt tgatctgtgt
     7561 caccagcgcc tcgccttgtt caggaacctg taacgttttt gccagttggc gaatgttggc
     7621 gtacatctgc tcaagggtgg ctggtacacg cggcagagtg acgacgttga ctttttgcgc
     7681 ccgcagttgg tcgagcacaa tttgcggtcc tgcatcctgc caggtaatta cgctatccgg
     7741 gcgaagtgac aaaatgcctt cgctgctgag ttgtttccag taaccaatat gcggcagttt
     7801 ggcggtttct ggtggatagg atgtcgtttc atcgacacca accacgcgtt tgccagcgcc
     7861 catcgcgtag attagctccg tcagcgatcc tcctgcgacc acgatacgtt cggcagccgt
     7921 tacgctaaaa gtccagctaa gagccagtaa tgccgtaaac aatttttttc gttttaccat
     7981 ggtcttcgcg gcagagacca cggctgaaat acataacgtt agtggggaaa aaagaaacgg
     8041 cctggtgatg atccttggca taatgtgaga actcttatca aattgagaat gatcatgatt
     8101 atcatttgag ttatatatga aatacaaccg tcgattcaat acgcacggcg ttacaagcgt
     8161 atttattttt gatttttatc aagatgcttc gtcagctaat gttcttgtta ccgattaaat
     8221 ttcagcgcgg gaaaaagctg agaacccgga gatatcgcgg cttgcagagc aaagagaaga
     8281 gaaaattatc actgttaacg acaacgtttt gtgatgaggt tgtgggggga tagccataaa
     8341 cacaggatgg ttttctggat gctggataga tttcataacc ccctgtttat tgtgttaatg
     8401 gcggttaaaa aaagcttgat cataaaaaat tgatcgataa taattctcat tatcacgttt
     8461 gagtttaaca aaatatgaca tcatggataa caaaatcata tcttttgtaa gggaaaacgc
     8521 acagcactct tcacttcaga cgtggcgtac tactttgcct gacaaagagg gcgtgctttt
     8581 gctgaattat cagaaatatt cggcaatttt acgggatata tacgctaata gcttcccgtg
     8641 gtgatatcta atcatttgaa ttgtaaataa atttattttt cgccagccac cccggtatta
     8701 acccaggcag ccagcatttt ttattttcct catggagaat cgctatgtca cgtccgcaat
     8761 ttacctcgtt gcgtttgagt ttgttggctt tggctgtttc tgccaccttg ccaacgtttg
     8821 cttttgctac tgaaaccatg accgttacgg caacggggaa tgcacgtagt tccttcgaag
     8881 cgcctatgat ggtcagcgtt atcgacactt ccgctcctga aaatcaaact gctacttcag
     8941 ccactgattt gctgcgtcat gttcctggaa ttactcttga tggtaccgga cgaaccaacg
     9001 gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt gctggttctt gtcgatggtg
     9061 ttcgccaggg aacggatacc ggacacctga atggcacttt tctcgatccg gcgctgatca
     9121 agcgtgttga gattgttcgc ggaccttcag cattactgta tggcagtggc gcgctgggtg
     9181 gagtgatctc ctacgatacg gtcgatgcaa aagatttatt gcaggaagga caaagcagtg
     9241 gttttcgtgt ctttggtact ggcggcacgg gggaccatag cctgggatta ggcgcgagcg
     9301 cgtttgggcg aactgaaaat ctggatggta ttgtggcctg gtccagtcgc gatcggggtg
     9361 atttacgcca gagcaatggt gaaaccgcgc cgaatgacga atccattaat aacatgctgg
     9421 cgaaagggac ctggcaaatt gattcagccc agtctctgag cggtttagtg cgttactaca
     9481 acaacgacgc gcgtgaacca aaaaatccgc agaccgttga agcttctgat agcagcaacc
     9541 cgatggtcga tcgttcaaca attcaacgcg atgcgcagct ttcttataaa ctcgccccgc
     9601 agggtaacga ctggttaaat gcagatgcaa aaatttactg gtcggaagtc cgtattaatg
     9661 cgcaaaacac ggggagttcc ggcgagtatc gtgaacagat aacaaaagga gcaaggctgg
     9721 agaaccgttc cactctattt gccgacagtt tcgcttctca cttactgaca tatggcggtg
     9781 agtattatcg tcaggaacaa catccgggtg gcgcgacgac gggcttcccg caagcaaaaa
     9841 tcgattttag ctctggctgg ctacaagatg agatcacctt acgcgatctg ccgattaccc
     9901 tgcttggcgg aacccgctat gacagttatc gcggtagcag cgacggctac aaagatgttg
     9961 atgccgacaa atggttatct cgtgcgggga tgactatcaa cccgaccaac tggctgatgt
    10021 tatttggctc atatgctcag gcattccgcg ccccgacgat gggcgaaatg tataacgatt
    10081 ctaaacactt ctcgattggt cgcttctata ccaactattg ggtgccaaac ccgaacttac
    10141 gtccggaaac taacgaaact caggagtacg gttttgggct gcgttttgat gacctgatgt
    10201 tgtccaatga tgctctggaa tttaaagcca gctactttga taccaaagcg aaagattata
    10261 tctccacgac cgtcgatttc gcggcggcga caactatgtc gtataacgtc ccgaacgcca
    10321 aaatctgggg ctgggatgtg atgacgaaat ataccactga tctgtttagc cttgatgtgg
    10381 cctataaccg tacccgcggc aaagacaccg ataccgggga atatatctcc agcattaacc
    10441 cggataccgt taccagtacc ctgaatattc cgatcgctca cagtggcttc tctgttggtt
    10501 gggtcggtac gtttgccgat cgctcaacac atatcagcag cagctacagc aaacaacctg
    10561 gctatggtgt gaatgatttc tacgtcagtt atcaaggaca acaggcgctc aaaggcatga
    10621 ccactactct ggtattgggc aacgccttcg ataaagagta ctggtcgccg caaggcatcc
    10681 cacaggatgg tcgtaacgga aaaattttcg tgagttatca atggtaatca tctgccccga
    10741 tatttcgggg catttatctg gaaggaagag agacaatgaa ccactacaca cgctggcttg
    10801 agttaaaaga acaaaatcct ggaaagtacg cgcgtgacat cgcaggttta atgaatatca
    10861 gcgaagcaga actggcattt gcgcgcgtca cgcacgacgc gtggcggatg cgcggcgata
    10921 ttcgtgaaat tctggcggcg ctcgaaagtg ttggcgaaac caaatgtatt tgccgtaatg
    10981 aatatgcagt ccatgagcaa gttggtgcgt tcacaaacca gcatttgaac ggacatgccg
    11041 gattgatcct caatccgcgc gcgctggatt tacgtctgtt tctcaatcaa tgggccagtg
    11101 ttttccacat caaagaaaac acggctcgtg gcgaacgcca gagtattcag ttctttgatc
    11161 atcagggcga tgcattacta aaagtttatg ccaccgacaa taccgatatg gcggcatgga
    11221 gtgagcttct ggcacggttt atcaccgatg agaatacgcc gcttgagtta aaagccgttg
    11281 atgcgccagt tgttcaaacg cgagccgatg ccagtgtggt cgagcaagag tggcgggcga
    11341 tgaccgacgt tcatcagttt tttacgttgc tcaagcgcca caacctgacg cgccaacagg
    11401 cgttcaatct ggtggcagac gatttggcct gcaaagtatc caacagtgcg ttggcgcaaa
    11461 ttcttgaatc tgcacagcag gatggcaatg aaatcatggt gtttgttggc aaccgtggct
    11521 gcgtacagat tttcaccggt gtggtagaaa aagtggtgcc aatgaaaggt tggctgaata
    11581 ttttcaaccc gacgtttacc cttcatctat tagaagagag cattgctgaa gcctgggtta
    11641 cccgtaaacc gaccagcgat ggccacgtaa ccagcctgga attgtttgcc catgatggta
    11701 cgcagatagc gcaactttat ggtcaacgta cagaaggtga acaggagcaa gcgcaatggc
    11761 gtaagcaaat tgcttcgttg ataccggaag gcgttactgc ataacgtata attaacggga
    11821 ggtctggctg ttgctgggcc tctttatatt gttcaatcgt tcacaccaga taattaatat
    11881 gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt
    11941 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca
    12001 tttgattcgc aatacagctc agaacctgat gctgcttgcg cgagagggac acatccgctt
    12061 caggcacaga gcagtaactg ccattgatga gtgcgacaaa gtcgatgatg gcgatagttc
    12121 gcggaacaaa ggtcaccggt actttcccca ggcagcgttt aatattaaag ggggagaaaa
    12181 taattacatg gacaggtttt aatcgtt
//
LOCUS       DAZFRE010000129        12113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFRE010000129 DAZFRE010000000
VERSION     DAZFRE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.129"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            139..561
                     /locus_tag="ACHKUI_004367"
     CDS             139..561
                     /locus_tag="ACHKUI_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600436.1"
                     /translation="MSKYIYILLSFLVLFFIFFYAYISLMSKEHHYTQHELSPFFLYT
                     PESLRNLPNISNVAEYSYYYNVDDMQTRVIVTWRNIDNIFLQKAKLIDFLKRMGPSLQ
                     NDCIWFFHDKSDYANNFQRYCIIEHRDSLQVEYFETIE"
     gene            716..1480
                     /gene="yabI"
                     /locus_tag="ACHKUI_004368"
     CDS             716..1480
                     /gene="yabI"
                     /locus_tag="ACHKUI_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6600437.1"
                     /translation="MQALLEHFITQSTVYSLMAVLLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1594..2292)
                     /gene="thiQ"
                     /locus_tag="ACHKUI_004369"
     CDS             complement(1594..2292)
                     /gene="thiQ"
                     /locus_tag="ACHKUI_004369"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6600438.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(2276..3886)
                     /gene="thiP"
                     /locus_tag="ACHKUI_004370"
     CDS             complement(2276..3886)
                     /gene="thiP"
                     /locus_tag="ACHKUI_004370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6600439.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIENIPGRNVKTD"
     gene            complement(3862..4845)
                     /gene="thiB"
                     /locus_tag="ACHKUI_004371"
     CDS             complement(3862..4845)
                     /gene="thiB"
                     /locus_tag="ACHKUI_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6600440.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESVQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYATANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4876..4975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5009..6664)
                     /gene="sgrR"
                     /locus_tag="ACHKUI_004372"
     CDS             complement(5009..6664)
                     /gene="sgrR"
                     /locus_tag="ACHKUI_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6600441.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     STMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTMKIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6753..6884
                     /gene="sgrT"
                     /locus_tag="ACHKUI_004373"
     CDS             6753..6884
                     /gene="sgrT"
                     /locus_tag="ACHKUI_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6600442.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6992..7597)
                     /gene="leuD"
                     /locus_tag="ACHKUI_004374"
     CDS             complement(6992..7597)
                     /gene="leuD"
                     /locus_tag="ACHKUI_004374"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6600443.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7608..9008)
                     /gene="leuC"
                     /locus_tag="ACHKUI_004375"
     CDS             complement(7608..9008)
                     /gene="leuC"
                     /locus_tag="ACHKUI_004375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6600444.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRSVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(9011..10102)
                     /gene="leuB"
                     /locus_tag="ACHKUI_004376"
     CDS             complement(9011..10102)
                     /gene="leuB"
                     /locus_tag="ACHKUI_004376"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6600445.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(10102..11673)
                     /gene="leuA"
                     /locus_tag="ACHKUI_004377"
     CDS             complement(10102..11673)
                     /gene="leuA"
                     /locus_tag="ACHKUI_004377"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6600446.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11765..11851)
                     /gene="leuL"
                     /locus_tag="ACHKUI_004378"
     CDS             complement(11765..11851)
                     /gene="leuL"
                     /locus_tag="ACHKUI_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6600447.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         2742 a         3210 c         3217 g         2944 t
ORIGIN      
        1 aaagataaaa acagaataaa tcatgagttg tatagttcat tatcaagttc gggagctata
       61 aagttttcag aatctccctt tgaaagaccg ctttttcttt tcttaaaccc tcattatccg
      121 cacaggtcgt gagtagagat gtcaaaatat atttatattt tattatcatt tttagtttta
      181 ttctttatat ttttctatgc ttatatctca ttgatgtcaa aggaacatca ctatacacag
      241 catgaacttt ccccattttt cctttatacg ccagagagtt tgcgaaattt gccaaacata
      301 tcaaacgtag ccgagtatag ctattactat aatgttgatg atatgcaaac aagagtgatt
      361 gttacatggc gaaatataga taacattttt cttcagaaag caaagctaat agattttttg
      421 aaaagaatgg gaccgagtct acaaaacgat tgtatatggt tttttcatga taaaagtgat
      481 tatgcaaata atttccagcg ttattgcatc attgagcacc gtgattctct gcaggtggaa
      541 tattttgaga ctatcgaatg ataggcagag ggtgtctttt gtgccggttg tgaagaaaaa
      601 gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga atcagataat tgacggcttg
      661 acggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc acattatgca
      721 agcattgctg gaacacttta ttacccaatc taccgtgtat tcattgatgg cggtgttatt
      781 ggtggccttt ctggagtcgc tggcgctggt cggtttgatt ctacccggta cggtgctgat
      841 ggcggggctg ggagcgctga ttggtagcgg tgagttaagt ttctggtatg cctggctggc
      901 ggggattatt ggctgcctgc tgggcgactg gatctccttc tggctgggct ggcgttttaa
      961 aaagccgttg catcgctggt catttctgaa gaagaacaaa gcattactcg ataaaaccga
     1021 acatgcgctg catcagcaca gcatgttcac cattctggtc ggtcgttttg tcggcccgac
     1081 gcgtccgctg gtgccaatgg tggcgggaat gctggatctc ccggtggcaa aatttattac
     1141 gccgaatatt atcggctgct tgctgtggcc accgttttac ttcctgccgg ggattctggc
     1201 gggtgcggcg atcgatattc ctgccgggat gcagagcggt gagtttaaat ggttgctgct
     1261 ggcaacagcg gtgtttttgt gggttggagg ctggctgtgc tggcggttat ggcgcagcgg
     1321 taaagcgact gaccgtttga gtcattattt gtcccgcggt cgtttgttgt ggctgacgcc
     1381 gttgatttct gccatcggcg tggtggcgct ggtggtgtta attcgccacc cgttgatgcc
     1441 ggtgtatatc gatattttgc gtaaagtggt tggggtttag gggatagcct tgtgcgggtt
     1501 gcctgatgcg acgcttgccg cgtcttatca ggcctgcaaa acgcactacc cgtaggtcgg
     1561 ataaggcgtt cacgccgcat ccgacagtgc atattaacct ttaattccca acagcgccga
     1621 agcactcgcc ttaccgctca acaactcatc ggtcttaccc tgccaggcga tacgcccgtc
     1681 ggcgaccacc accgagcgcg tggcgatccg cgccgcatct tccacactgt gcgacaccat
     1741 caagagcgtc attttttgct gttggcagct cgtgctcacc agcgtcagca tctcctgacg
     1801 tagcgccgga tcgagcgcgg aaaacggttc atcgagcaat aaaatgggct gttcgcgtac
     1861 cagacaacgc gctaacgcca cgcgctgtcg ttgaccgccg gaaagctcgc caggtaaccg
     1921 cgccattaaa ttatcaatcc ccatctggtg ggcaatcgcg tgcattttct tctgctgcgc
     1981 cgcatttagt ttcaaccccg gatttagccc cagcccgatg ttctgcgcga ccgtcaggtg
     2041 gctgaacagg ttgttctcct gaaacagcat cgacaccgga cggcgtgacg gcggcgtcgt
     2101 ggtgtgatca acgccgtcga tagtcagcaa accgctggct ggcgtcagaa aaccggcgat
     2161 caaattaagc agggtacttt tacctgcgcc gcttggcccg aggatcgcca cctgctcgcc
     2221 gcgttccacc gttaagctaa aacgcatcgg caaatggtgg taaagccagg tgatatcagt
     2281 cagttttaac atttcgcccc ggtatatttt caatcacggt aaacagcaga aaacagagca
     2341 gcagcagaat taacgcggtg accgcgccgt cctggctgcg ataggagcca atttgctggt
     2401 agagataaaa cggcagggta cggaaatcat cgttaccaaa caatgccacc acgccaaaat
     2461 caccaatcga cagcacacaa gcaaaggcca gcgcctgcgc cagtggacgt ttcagggcgc
     2521 gcagctccac cacttttaag cgtgaccagc cttcaatgcc cagcgactga cacaacatgc
     2581 tgtagcgggc ggtgatatcg cgcatcgggt tttccagtac tttcagcgca taagggattg
     2641 ccattaacgc attggtgaaa atcacaatgc cgtcagcaga ttgtggcagg ccgatagtgt
     2701 tgttgagcag taaaaagaag cccgtagcca gcacaatccc cggcatggcg aggatcaaca
     2761 tgccgctcat ctccagcgcc tgacccgcca gcattttctg ccgcgcccga agttcgcgac
     2821 tgctccatag cagcatcatg gtcagcacta cgcacaatac gcctgccgcc agcgcaatac
     2881 gcaacgaggt ccacagcgcc tgccacagca ccggttgtgc cagcacttcc ggcaactggc
     2941 gatttacccc atcgacgatc accgccagta acggtggcag caacagcagc agcgccagca
     3001 caattaacgc cgtgtcgcaa atgcgactat gcagacgatc gtccgggtcg cgccagcctt
     3061 gcagcagtgt ggtaccaggc gcaatggcct tactcaatcg ctgactcaac agcaccagcc
     3121 cgaggcagca caccatctgg atcagcgcca gcattgccgc gcgggcagga tcgtagtcgt
     3181 aactcagtgc ctgatagatt gccagctcga tagtggtcgc ctgcggaccg ccgcccagcg
     3241 atagcacggt ggcgaagctg gcgaaacaga gcataaagat tagtgcggca accggcggga
     3301 tttgtcgccg taaccacggc cattcgacga aacggaaaaa atgccagccg cgcatcccaa
     3361 gttgggcggc aagctgacgt tgttcgccgg gaatgttttc cagtgcctgg agtaataaac
     3421 ggctagccat cggcagatta aaaaacacat gggccagcaa aatgccttgc aggccgtagg
     3481 gcgaaaaggt ccactccaga ccaagtgatt gccagagcga ggccagccag ccctggcgac
     3541 catagacgct aagaatgcca aaaacagcaa ccagcaccgg gaggatcaag gtcattgcgc
     3601 acagacgcaa cagcatctgt cgcccaggaa agcgcctgcg atagagggcg cgggcgagga
     3661 atattgcggg tacgacagag agcagcgccg agagaaacgc ctgccagaag gagaagcgca
     3721 ccacatgcca caggtagctg tcctgccaga ctgccaccca gtcaccctgc ggcgcgttcc
     3781 accacagggc gagaaacgcc gccagtgcaa ccgccaccac cagcgtggcg gcgcttacgc
     3841 ctggaattaa ccagccggga attaacggct gacggcgcgt tgccattcgc taatccatgc
     3901 ctgacgttgt gccgccactt cggcgggtgt gaactctagc gtggtggctg gtttggtcaa
     3961 ttgttcaaat cctgcaggca gagtgacgtt tgctaccgga tacatccagt tgccggttgg
     4021 gatcgcattc tgaaaagccg gagaaaccat aaactggagg aatttttgcg ccagctctgg
     4081 ctgcttgctg gcagcggtgc gggcggcgac ttccacttgc aaatagtgac cttcgctgaa
     4141 gttcgcggtg gcgtagttat ctttcttctc ttcgagaatg tgataagccg gagaggtggt
     4201 gtaactcagg accagatcgc tttcaccttt taaaaacagg ccgtaggctt cgctccagcc
     4261 tttggtgacc gtgaccgttt tcttcgccag tttctgccag gcttgcgggg cgttatcgcc
     4321 atagactttt tgcatccata gcaacagacc cagccccggt gtactggtac gtggatcttc
     4381 ataaatcacc cgccagtttt gaacgctctc aaccaattct ttcaggcttt gcggcggatt
     4441 tttcagtttg ttcttgtcat agacgaaggc gaaatagccg taatcaaacg gtacgaaagt
     4501 gtcattattc cagccgccag gaacgttaac ggcttccgct gccacaccgc ttttagcaaa
     4561 cagcccggtt ttgctggcgg cgtctaacag attgttatcc agccccagca ccacatcggc
     4621 tttgctgttt ttgccttcca tccgtagacg gttgagcagc gaaacgccat cttccagcgc
     4681 caccagtttc agttcgcaat tacagtctgc ttcaaaggct tttttgacta ccggaccagg
     4741 tccccagtca gcggcaaagg aatcgtaggt ataaacagtc agaacaggtt tagcgaaaac
     4801 aggcgctgtg cacagcaaca gcaggggaag acattttttt aacactttgc acctcacaaa
     4861 agagtggcaa aggacttgag aaggagcctc aaatcccttc gccggcgtta tccggatcag
     4921 gttcgacggg tattttctca gcgcacgcgg ctgcgtggca ccccgttgag aacggcgtta
     4981 gtgtagtgat tttgttatca accagcaatc atggatccgg tggtgcaaac cacgctgatt
     5041 taaaatcgaa ccagccgagg gtgttcatgc gcaggccgcg catactacgt tgcccctgaa
     5101 tgatcagcca gtggtggata agcggcacca ttgctttgct ggcgaccagt tgctggcacc
     5161 agttcgccag gttcatttcg ccattgcgcc agcgggcggc gtcggcttgc cagtcgatcg
     5221 gaatacagtg ttggagcagc ggcacctcgc acaagtgcgc gaacagcgaa aaatccagcg
     5281 gcagcgtaaa gttggcgctg ttaagccaga tatcgctctc gatctctcct tcatgccact
     5341 gatcgtagct gatctctttg attttcatcg tgacctggtg acttgccaga atctgctgca
     5401 tgatcccggc aatcacccga tgctcgctgt gatcctgata aaaggtcagg gtgaggcttt
     5461 ccagaccagc cggtttttcg ctctttatgg tgcgggcatg gtgccaacgg gggagcagtc
     5521 cataagccgg gaaccacagt tgctggtatt gttcctcggc gaaatagacc agattcgtcg
     5581 gagaaagcac atagcttacc cagtctctga cttgctgatt cgcccctcga tgggtgcggc
     5641 tgtcgaacag taaatagtag caaccttcct ccaggcggct ttcaatctct ttttcctcgc
     5701 cctgtggacc ttttagcatc agccctccgg ctggctcgtc ggcaatttcc ggcagaaccc
     5761 agacgttaac ttcgtcgatt aatgcccggt aaccgaagaa gtcatcgaat gcctgaattt
     5821 tcagttgatt ggtgctgttg cggatcaccg catacggacc ggtgccgatg ggatggctgg
     5881 caaagttact gagggtttcc cattcgcgcg gcaggatcat tgccggaact tgccccagca
     5941 gtaacggcaa ccagcggtcc ggttgcgtga gatggatatc cagtgtccag ggcgtcggcg
     6001 acacaatgtc agcaatatgc gaatagagcg gaagcgtatt gattcgtttt aaagaggcga
     6061 tcacatcgtc catttccagt tcacgaccat ggtgaaaatg gactcctgga cgcaaaaaga
     6121 aacgccagtg aagcggtgat atttgctgcc agtggtgggc gatgtctgct tccagttccc
     6181 cattttcctc atttatgcgc gttagcgaac tgaagatttg ccgggcgata tgggtttcag
     6241 aacggcgcaa tgcgctgcca ggtagcagat tacgcaacgg acgatagtag agcacgcgca
     6301 ggatgtgccg cccctggcgg aagctgcggc ccagatgaga aaccagcatt tgccgcacag
     6361 tcgctttgtc gccaaccaac tgcactagtt ggtcgatacg atcctgctcc agcaggtctt
     6421 ccgcccgctg ttgctgaagc gccagcccgg tatagaggaa tgtcagacgc gagcgtttac
     6481 cgcgcccgac ttccgcttcc cacgtcagcc agccgcgatc ctgcatggtg ctgagcaggg
     6541 tgcgcatatg acgacgcgag caactcagta acgctgccag ttcgttgagc gtggtctcct
     6601 gagatttacc ctcgcagcat tgccacaggc ggatgaactg ttgttgcaga cgagcagatg
     6661 gcataaaagg ggaactcctg cgcaaaagac agcaatttta ttttccctat attaagtcaa
     6721 taattcctaa cgatgaagca aggaggtgcc ccatgcgtca gttttatcag cactatttta
     6781 ccgcgacagc gaagttgtgc tggttgcgtt ggttaagcgt cccacaacga ttaaccatgc
     6841 ttgaagaact gatgcaatgg gaggggagtc attctgacta ctgatttgcg gacatcatgt
     6901 gtgactgagt attggtgtta atcgccacgc cagcagtgat tatctgctgg cttttttctt
     6961 tttggcgatt gagtgccgga cggtgtacaa actaacgcat aaacgccggt tgcttctctt
     7021 cataagacgc aattgcgtcg tcgtgctgca gagtcaggcc gatgctgtcc agaccgttca
     7081 tcatgcagtg gcggcggaag gcatcgatgg taaagcgata agttttctct cccgctttca
     7141 cctcttgcgc ttccaggtct acatcaaaat gaatccccgg attggctttc accagcgcaa
     7201 acagctcatc cacttctgca tcgcttaatt tcaccggcag cagctggttg ttaaagctat
     7261 tgccgtagaa gatgtcagca aaactcggcg caatcaccac cttaaaaccg taatcggtca
     7321 atgcccacgg cgcgtgctca cgcgaggaac cgcagccgaa gttttctcgt gccagcaaaa
     7381 tggaagcgcc ctgatactgc gggaagttca gcacgaagtc cgggtttggc tgttggcctt
     7441 tttcatccag aaaacgccag tcgttaaaca gatgcgcgcc aaaacccgta cgggtcactt
     7501 tctgcaaaaa ctgtttcggg atgattgcat cggtatcgac attggcggca tccaacggaa
     7561 ccaccaggcc tgtgtgtttg ataaatttct ctgccatggt gtgctcctta tttaatgttg
     7621 cgaatgtcgg caaaatgacc ggtcacagcc gcggcggcag ccattgccgg gctgaccaga
     7681 tgcgtgcgcc cgccgcgtcc ctggcggcct tcaaagttac ggttgctggt ggaggcgcaa
     7741 cgttcgcccg gattcaggcg gtcgttgttc atcgccagac acattgaaca acctggcaag
     7801 cgccattcaa aaccagcttc gataaaaatt ttgtccagac cttccgcttc cgcctgggct
     7861 ttaaccggac cggaaccggg aaccaccagc gcttgtacgc ctggcgcgac tttccggcct
     7921 ttggcgattt ccgctgctgc acgtaaatct tcaatacgcg agttagtaca ggaaccaata
     7981 aacactttgt cgatagccac ttctgtcagc ggaatacccg gtttcagccc catataggcc
     8041 agcgcttttt ctgccgacgc gcgttcaacc ggatcggcaa acgaagccgg atcgggaata
     8101 ttgtcgttca cagaaatcac ctggcccgga ttggtgcccc aggtgacctg cggcgaaatc
     8161 tcttctgctt gcagagtgac aacggtatcg aaagttgctc cttcgtcggt ttgcagggtt
     8221 ttccagtagg caacggcgtc gtcgaaatct ttgcctttcg gcgcatgcag acggccttta
     8281 acatagttaa aggtggtttc gtccggtgca accagaccgg cttttgcgcc catttcgatt
     8341 gccatattgc acagggtcat acgaccttcc atgcttaaat cacggattgc ttcgccgcaa
     8401 aattccacca catgcccggt accgcctgcg ctgccggttt taccaataat tgccagcacg
     8461 atatcttttg cggtaatgcc cggcgcggct ttgccctgga cttcaatttt catggtcttc
     8521 gcccggccct gtttcagggt ttgcgttgcc agtacgtgtt caacttcgga agtgccgata
     8581 ccaaaggcca gtgcgccaaa cgcgccgtgg gtggcggtat gtgagtcgcc gcagacaatg
     8641 gtcatccccg gcaaggtcac gccctgttcc ggccccatta cgtggacgat cccctgatac
     8701 gggtgattca ggtcatacag ctcgacgcca aattctttgc agtttttgat cagctcctgc
     8761 atctggatgc gcgccatttc accgcaggca ttaatgtctt tcgtctgggt agagacgttg
     8821 tgatccatgg tggcgaaggt tttgcccggc tgacgtaccg agcgaccgtg ggcgcgcaga
     8881 ccatcgaacg cctgcggtga ggtcacttca tgcaccaggt ggcggtcgat atataacagc
     8941 ggggtttcgt tttcggcttc gtacactacg tgagcgtcga ataatttttc gtataacgtc
     9001 ttagccatga ttacacccct tctgccacat agcgggcaat gatatcgccc atttcatcgg
     9061 tactaacggc ggcagcgcca cgggctaaat ccccggtgcg aatgccttct tctaatgcgc
     9121 ggttaatggc gcgttcaatg gcggaagccg catcatcggc atccaggctg taacgcagca
     9181 gcagcgccag cgacagaatt tgtgcaatcg ggttggcgat atttttacct gcgatatcgg
     9241 gtgccgagcc gcccgccggt tcatacagac caaaaccttg ctcgttcagg ctggcggaag
     9301 gcaacatccc cattgagcca gtgatcattg cgcactcatc agacagaatg tcgccaaaca
     9361 ggttggagca cagcaagacg tcaaactgtg atggatcttt aatcagctgc atggttgcgt
     9421 tgtcgatgta catatgcgcc agttcgacgt ccgggtattc cgtggcgatc tcgttaacga
     9481 tctcccgcca taaaatagag gattgcagca cgttggcttt gtcgattgag gtgactttgt
     9541 ggcgacgctt gcgggcagat tcaaatgcga tgcgggcaat acgctcgatc tcaaaacggt
     9601 ggtacacctc ggtatcaaac gctttttcat attgtccgct accttcgcgg ccttttggct
     9661 gaccgaaata gatgccgccg gtcagttcgc gcacacacag gatgtcaaag ccgtttgcgg
     9721 caatgtcagc acgcagcgga cagaatgctt ccagcccctg atacagtttt gccggacgca
     9781 gattgctgaa taatttgaag tgcttacgca aaggcagcag tgcgccgcgt tctggttgct
     9841 ggtctggcgg caaatgttcc cacttcggac cacctaccga gccaaacaac acggcatcgg
     9901 cttgctcgca accttcaact gtcgcaggcg gcagtggctg cccgtggtta tcaatggcgg
     9961 cgccgcctac atcgtaatgg ctggtggtga tacgcatcgc aaatcggttg cgcacggcat
    10021 ccagcacttt cagcgcctgg gtcatcactt ccggaccaat accgtccccc ggcaatacgg
    10081 caatatggta attcttcgac atcacacggt ttccttgttg ttttcgttgt gttgagcttt
    10141 gcgttgcaac tctttttcga cttctgcggc acgccagata ttgttcagta cgtgcaccat
    10201 ggctttggcg gaggactcga caatatcggt ggccaggccg acgccgtgga agcggcgacc
    10261 gttgtagttg gcgacaatat ccacctgacc cagcgcatct ttaccgtggc ctttggcggt
    10321 caggctgtat ttcaccagtt cgacgttata gtcagtgatg cggtttatcg cctggtagac
    10381 ggcatcgacc ggaccgttac cgttggcggc ttctgctttg acttcttcgc cacaggccag
    10441 tttgacggcg gcagtggcaa tatcgttaga acctgactgc acgctgaagt aatccagacg
    10501 gaaatgctcc ggctcttctt gctgcttacc gatgaaggcc agcgcctcca gatcgtaatc
    10561 aaacacctgg ccttttttgt ccgccagctt caggaaagcg tcgtacaggt tgtctaaatt
    10621 atattcactt tctttatatc ccatctcatc catgcgatgt ttcaccgccg cacgccccga
    10681 acgagaggtc agattcagct ggatttggtt cagaccaata gattctggtg tcatgatttc
    10741 gtagttttcg cggtttttca gtacaccatc ctggtggata ccggaggagt gtgcgaatgc
    10801 gccgctgcca acaatggctt tgtttgccgg gatcggcata ttacaaatct ggctaactaa
    10861 ctggctggtg cgccatatct cctggtgatt gatggcggtg tggacgttga gaatatcctt
    10921 acgaactttg atcgccatga tgacttcttc aagggaacag ttaccggcac gctcgccgat
    10981 cccgttcatt gcgccttcta cctgccgtgc tccggcatgt accgccgcca gtgagttgcc
    11041 gaccgccagg cccaaatcgt cgtgggtatg tacggaaata atggctttgt cgatgttggg
    11101 tacgcgttca tacaggccgc tgatgattcc ggcgaactca aatggcatgg tgtagcccac
    11161 ggtgtccgga atgttgatgg tggtggcacc ggcattaatc gccgcttcga ccactcgcgc
    11221 cagatcggca attggggtgc gtccggcatc ttcgcaagaa aattcaacat catcggtgta
    11281 attacgggcg cgtttcacca tatagatagc gcgttcgatc acttcgtcca gcgtgctgcg
    11341 cagcttggtg gcgatgtgca ttggcgaagt ggcaataaaa gtatggatac ggaaggcttc
    11401 ggcgactttc agggattcgg ctgccacgtc aatatctttt tccacgcagc gagctaacgc
    11461 gcatacacgg ctgtttttaa cctggcgagc gatggtctgc actgattcaa aatcacccgg
    11521 cgaagagacg gggaaaccga cttccatcac gtcaacaccc atacgctcaa gggccagcgc
    11581 aatttgcagt ttttctttca cactcaagct tgcctgtaac gcctgttcac cgtcgcgcaa
    11641 tgtggtatcg aaaataatga cttgctggct catggtttgg gtccttgtct ctttttgagc
    11701 gcctcgcttc gggcataaaa aaacccgcgc aatggcgcgg gtttttgttt gactgcgtgc
    11761 tggcttaatg ctggatgccg ctcactcgtc taccgcgcaa agaagatgcg tttagtagta
    11821 gtagaccgat aaagcgaacg atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc
    11881 ttttagagtt actggataca aaaacggatg tcaaccctga agcaataaaa acgtcacgcc
    11941 attgtgagtt cggcatccgt aaaattagct aattgtgctg cggtggttaa aacaagcgat
    12001 attaatttct gcttaaccgc cgatgctttt catcggttga catatttcag aataattttt
    12061 tgcatctaat caacgaggga aagggggata aaatgcacgc gttacaaaac cta
//
LOCUS       DAZFRE010000130        11868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFRE010000130 DAZFRE010000000
VERSION     DAZFRE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.130"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            67..450
                     /locus_tag="ACHKUI_004379"
     CDS             67..450
                     /locus_tag="ACHKUI_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFO6600448.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="ACHKUI_004380"
     CDS             complement(514..957)
                     /locus_tag="ACHKUI_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6600449.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ACHKUI_004381"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ACHKUI_004381"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6600450.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="ACHKUI_004382"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="ACHKUI_004382"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6600451.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCAGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="ACHKUI_004383"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="ACHKUI_004383"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6600452.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5296..7020
                     /gene="aceK"
                     /locus_tag="ACHKUI_004384"
     CDS             5296..7020
                     /gene="aceK"
                     /locus_tag="ACHKUI_004384"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6600453.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7037..7861)
                     /gene="iclR"
                     /locus_tag="ACHKUI_004385"
     CDS             complement(7037..7861)
                     /gene="iclR"
                     /locus_tag="ACHKUI_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6600454.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8061..11744
                     /gene="metH"
                     /locus_tag="ACHKUI_004386"
     CDS             8061..11744
                     /gene="metH"
                     /locus_tag="ACHKUI_004386"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6600455.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVVHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2881 a         3036 c         3186 g         2765 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct atggtattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag taagtgaata aattttattt atattgttat
     2161 caataagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttt acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaacgttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcggt taacggcacc atcagctata gcaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgagaca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccgagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtgc
     3541 tggtgaacgc accgaagaag gtatgcgcgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccatgggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggtggtcag gcgctgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagcgg tggtggagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cttcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggcga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcatc agcaggaagt gggtacaggt
     5161 tacttcgata aagtgacgac cattattcag ggcggcacgt cgtcggttac cgcactgact
     5221 ggctccactg aagaatcgca gttctaacaa ccaacgcccc tttccgtaag gcgaggggcg
     5281 aattaaggag aggcgatgcc gcgtggcctg gaattactga ttgcacaaac cattttgcaa
     5341 ggtttcgacg ctcagtatgg tcgattcctc gaagtgactt ccggggcgca gcagcgtttc
     5401 gagcaagccg actggcatgc tgtccagcag gcgatgaaaa accgtattca tctttacgat
     5461 catcacgtgg gtctggtcgt ggagcaactg cgctgcatta ctaacggcca aagcacggac
     5521 gcggcatttt tactgcgcgt taaagagcat tacacccggc tgttgccaga ttacccgcgc
     5581 ttcgagattg cggagagctt tttcaactcc gtgtactgtc ggttatttga ccaccgctcg
     5641 cttactcccg agcggctttt tatctttagc tcccagccag agcgccgctt tcgtaccatt
     5701 ccacgcccac tggcgaaaga ctttcacccc gatcacggct gggaatctct gctgatgcgc
     5761 gttatcagcg atctgccgct gcgtctgcgt tggcagaata aaagccgtga cattcattac
     5821 atcgttcgcc atctgacgga aacgctgggg acagacaacc tcgcggaaag tcatttacag
     5881 gtggcgaacg aactgtttta ccgcaataag gcagcctggc tggtgggcaa actaataacg
     5941 ccttccggca cgttgccatt tttattgccg atccaccaga cggacgacgg cgagttattt
     6001 attgatacct gcctgacgac gaccgccgaa gcgagcattg tttttggctt tgcgcgttct
     6061 tattttatgg tttatgcacc gctgcccgca gcgctggtcg agtggctacg ggaaattctg
     6121 ccaggtaaaa ccaccgctga attgtatatg gctatcggct gccagaagca cgccaaaacc
     6181 gaaagctacc gcgaatatct cgtttatcta cagggctgta atgagcagtt cattgaagcg
     6241 ccgggtattc gtggaatggt gatgttggtg tttacgttgc cgggttttga tcgggtattt
     6301 aaagtcatca aagacaggtt cgcgccgcag aaagagatgt ctgccgctca cgttcgtgcc
     6361 tgctatcaac tggtgaaaga gcacgatcgc gtgggccgaa tggcggacac ccaggagttt
     6421 gaaaactttg tgctggagaa gcggcatatt tccccggcat taatggaatt actgctccag
     6481 gaagcagcgg aaaaaatcac cgatctcggc gaacagattg tgattcgcca tctttatatt
     6541 gagcggcgga tggtgccgct caatatctgg ctggaacaag tggaaggtca gcagctgcgc
     6601 gacgctattg aagagtacgg caacgccatt cgccagcttg ccgctgccaa catcttccct
     6661 ggggatatgt tatttaaaaa ctttggcgtc acccgtcacg ggcgcgtggt gttttatgat
     6721 tacgacgaaa tttgctacat gacggaagtg aatttccgcg acatcccgct gccgcgctac
     6781 ccggaagacg aacttgccag cgaaccgtgg tacagcgtct cgccgggtga tgttttcccg
     6841 gaagagttcc gtcactggtt atgtgccgac ccgcgtattg gtccgctatt tgaagagatg
     6901 cacgccgact tgttccgcgc tgattactgg cgcgcactac aaaaccgcat acgtgaaggg
     6961 catgtggaag atgtttatgc gtatcggcgc aggcagcggt ttagcgtccg attcgtataa
     7021 atcagaggcg aaaaagtcag cgtattccac cgtacgccag cgtcacttcc ttcgccgctt
     7081 taatcaccat cgcgccaaac tcggtcacgc ggtcatcggt aatacgtgaa atcggtccgg
     7141 aaattgaaat tgcggcaaac ggttcgcggt gctcatcgaa aatacacgct gcaaggcaac
     7201 gcagccccag cgcatgttcc tcatcgtcaa atgaataacc ccgtttgcgc gtttgggcga
     7261 gatcttcttt taaatgcaca ggagacacca gcgtcgcgtg ggtataggca tgtaaccctt
     7321 tgcggtgcag cagcttcgtc acctgttctt cgctcagttg ggccagaaag gctttacccg
     7381 caccggaagc gtgcatcggc aatttaccgc cgataggcgc ggacattcgc atcaggtgag
     7441 tacactgtac ctggtcgata ataatcgctt cgtgattgct ttgatcaagc accgccatat
     7501 tgaccgtttc gccagactct tccattagat tgcgcagaat aggatggaca atcgctaaca
     7561 aattacggct ctggagaaag ctgctaccga ccataaaggc gtgtgcgccg attgcccagt
     7621 gacccagttc gccgacctga cggacaaagc cctgttgctg catcgtggtt agcaggcggt
     7681 gggtcgtgga attgggtaac ccggcttgct gtgccagttc cgtgagtgcc acactgccat
     7741 tggattcggc aatccactcc agtaatttca ggccacgcgt taaagactga acctgtccag
     7801 tcgctggtgc ggtggcaacg gcgggttttc tgccgcgttt tgcgggaatg ggtgcgacca
     7861 tgacagtctc ctttttctgt atcgtggaaa tcattttcat ttttattatc agcgaatgca
     7921 atagttgctg aacagatccg atgagttaat gttgaacaaa tctcatgttg cgtggtggtc
     7981 gcttttacca cagatgcgtt tatgccagta tggtttgttg aatttttatt aaatctgggt
     8041 tgagcgtgtc gggagcaagt gtgagcagca aagtggaaca actgcgtgcg cagttaaatg
     8101 aacgtattct ggtgctggac ggcggtatgg gcaccatgat ccagagctat cgactgaacg
     8161 aagccgattt tcgtggtgaa cgctttgccg actggccatg cgacctcaaa ggcaataacg
     8221 atctgctggt gctcagtaaa ccggaagtga tcgccgctat ccacaacgcc tactttgaag
     8281 cgggcgcgga tatcatcgaa accaacacct tcaactccac gaccattgcg atggcggatt
     8341 accagatgga atccctgtcg gcggaaatca actttgcagc ggcgaaactg gcgcgcgcct
     8401 gtgccgacga gtggactgcc cgcacgccgg agaaaccgcg ctacgttgcc ggtgttctcg
     8461 gcccgaccaa ccgcacggcg tctatttctc cggacgtcaa cgatccggca ttccgcaaca
     8521 tcacttttga ccagctggtg gcggcttatc gagaatccac caaagcgctg gtggaaggtg
     8581 gcgcggacct gatcctgatt gaaaccgttt tcgacaccct taacgccaaa gcggcggtct
     8641 ttgcggtgaa aacggagttt gaagcgctgg gcgttgaact gccgattatg atctccggca
     8701 ccatcaccga cgcctccggg cgcacgctct ccgggcagac cactgaagcg ttttacaact
     8761 ccctgcgcca cgccgaagcg ctgacctttg gcctgaactg tgcgctgggg ccagacgaac
     8821 tgcgccagta cgtacaggaa ctgtcgcgta ttgcggaatg ctatgtcacc gcccacccga
     8881 acgccgggct gcccaacgcc tttggtgaat acgatctcga cgccgacacg atggcaaaac
     8941 agatacgtga atgggcagaa gcgggctttc tcaatatcgt cggcggctgc tgcggcacca
     9001 cgccacaaca tattgcagcg atgagtcgtg cagtagaagg attagcgccg cgcaaactac
     9061 cggaaattcc cgtagcctgc cgtttgtccg gcctggagcc gctgaacatt ggcgaagata
     9121 gcctgtttgt gaacgtgggt gaacgcacca acgtcaccgg ttccgctaag ttcaagcgac
     9181 tgatcaaaga agagaaatac agcgaggcgc tggatgtggc gcgtcagcag gtggaaaacg
     9241 gtgcgcagat tatcgatatc aacatggatg aagggatgct cgacgccgaa gcggcgatgg
     9301 tgcgttttct caatctgatt gccggtgaac cggatatcgc tcgcgtgccg attatgatcg
     9361 actcctcaaa atgggacgtt attgaaaaag gtctgaagtg tatccagggc aaaggcattg
     9421 ttaactctat ctcgatgaaa gagggtgtcg acgcctttat ccatcacgcg aaactgttgc
     9481 gtcgctacgg tgcggcagtc gtggtaatgg cttttgacga acagggccag gccgataccc
     9541 gcgcacggaa aatcgagatt tgccgtcggg cgtacaaaat cctcaccgaa gaggttggct
     9601 tcccggctga agatatcatc ttcgacccga acatcttcgc ggtcgcaact ggcattgaag
     9661 agcacaacaa ttacgcgcag gactttatcg gcgcgtgtga agacatcaaa cgtgaactgc
     9721 cgcacgcact gatttccggc ggcgtttcta acgtttcttt ctcattccgt ggcaacgatc
     9781 cggtgcgcga agccattcac gcggtgttcc tctactacgc tattcgcaat ggcatggata
     9841 tggggatcgt caacgccggg caactggcga tttacgacga cctgcctacc gagctacgcg
     9901 acgcggtgga ggatgtgatc ctcaaccgtc gcgacgatgg caccgagcgt ttactcgagc
     9961 ttgccgagaa atatcgcggc agcaaaaccg acgacaccgc taacacccag caggcggagt
    10021 ggcgctcgtg ggaagtgaat aaacgtctgg aatactcgct ggtcaaaggc attaccgagt
    10081 ttatcgagca ggataccgaa gaagcccgcc agcaggctac gcgtccgatt gaagtgattg
    10141 aaggcccgtt gatggacggc atgaacgtgg tcggcgacct gtttggcgag ggcaaaatgt
    10201 tcctgccgca ggtggtgaaa tcggcgcgcg taatgaagca ggcggtggcg tatctcgaac
    10261 cgtttattga agccagcaaa gagcagggta aaaccaacgg caagatggtg atcgccaccg
    10321 tgaaaggcga tgtccacgac atcggtaaaa atatcgttgg tgtggtgctg caatgtaaca
    10381 actacgaaat tgtcgatctc ggcgtgatgg tgcctgcgga aaaaattctc cgtaccgcta
    10441 aagaagtgaa tgctgatttg attggccttt cggggctgat cacgccgtcg ctggacgaga
    10501 tggttaacgt ggcgaaagag atggagcgtc agggcttcac cattccgcta ttgattggcg
    10561 gcgcgaccac ctcaaaagcg cacacggcgg tgaaaattga gcagaactac agcggcccga
    10621 cggtgtatgt gcagaacgct tcacgcaccg ttggtgtggt ggcggcgttg ctttccgaca
    10681 ctcagcgtga tgatttcgtt gctcgtaccc gcaaggagta cgaaaccgtg cgtattcagc
    10741 acggacgaaa gaaaccacgc acaccaccgg tcacgctgga agcggcgcgt gataacgact
    10801 tcgcttttga ctggcaggct tacacgccgc cggtggtgca ccgtctcggt gtgcaggaag
    10861 tcgaagccag catcgaaacg ctgcgtaatt acatcgactg gacgccgttc tttatgacct
    10921 ggtcgctggc cgggaagtat ccgcgcattc tggaagatga agtggtaggt gttgaggcgc
    10981 agcggctgtt taaagacgcc aacgacatgc tggataaatt aagcgccgaa aaaatgctga
    11041 acccgcgtgg tgtggtgggc ctgttcccgg caaaccgtgt gggcgatgac attgaaatct
    11101 accgtgacga aacgcgtacc catgtgatta atgtcagcca ccatctacgc caacagaccg
    11161 aaaaaaccgg cttcgctaac tactgtctcg ctgacttcgt tgcgccgaag gtttccggca
    11221 aagcggatta catcggcgca tttgccgtga ctggcgggct ggaagaggac gcactggctg
    11281 atgcctttga agcacagcat gatgattaca acaaaatcat ggtgaaagcg cttgccgacc
    11341 gtctggcgga agcctttgcg gagtatctcc atgagcgtgt gcgtaaagtc tactggggct
    11401 atgcgccgaa cgagaacctc agcaacgaag agctgatccg cgaaaactac cagggcatcc
    11461 gtccggcacc gggctaccct gcctgcccgg aacatacgga aaaagccaca atctgggagc
    11521 tgctggaagt ggaaaaacat accggtatga aactcacgga atccttcgcc atgtggccgg
    11581 gtgcatcggt ttcgggttgg tacttcagtc atccagacag caagtattac gcggtggcgc
    11641 aaattcagcg cgatcaggtt gaagactatg cccgccgtaa aggaatgagc gttaccgaag
    11701 ttgagcgttg gttggcaccg aatctggggt atgatgccga gtaagtgata acaccggggt
    11761 atttgccccg gtttttaatg tcataaataa tggattattg aacatgataa aaattgatga
    11821 aatagatttt tttgctttcg aaggatgttc agagtccgaa gttactga
//
LOCUS       DAZFRE010000131        11850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFRE010000131 DAZFRE010000000
VERSION     DAZFRE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.131"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            537..725
                     /locus_tag="ACHKUI_004387"
     CDS             537..725
                     /locus_tag="ACHKUI_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO6600456.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
     gene            846..1586
                     /locus_tag="ACHKUI_004388"
     CDS             846..1586
                     /locus_tag="ACHKUI_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6600457.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWPVTDETMRNWLKQAV
                     KRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(1829..2806)
                     /locus_tag="ACHKUI_004389"
     CDS             complement(1829..2806)
                     /locus_tag="ACHKUI_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6600458.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3981..4622)
                     /locus_tag="ACHKUI_004390"
     CDS             complement(3981..4622)
                     /locus_tag="ACHKUI_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600459.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELAS
                     QTLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIADPARDYIDLTLDQFPGYHNRIVA
                     EPVESGGQLAADLNREPAISTADGIVASPSDKVNYITEQKNRQPMVIITECYKLIRMT
                     ARFIPMCQSSQLFQHQVFPRLFIISDVGSCSLFRNTLDRLATDWKVTLSILSL"
     gene            6944..7162
                     /gene="ccdA"
                     /locus_tag="ACHKUI_004391"
     CDS             6944..7162
                     /gene="ccdA"
                     /locus_tag="ACHKUI_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO6600460.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            7164..7469
                     /gene="ccdB"
                     /locus_tag="ACHKUI_004392"
     CDS             7164..7469
                     /gene="ccdB"
                     /locus_tag="ACHKUI_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO6600461.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            7470..8279
                     /locus_tag="ACHKUI_004393"
     CDS             7470..8279
                     /locus_tag="ACHKUI_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6600462.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
     gene            complement(8417..8692)
                     /locus_tag="ACHKUI_004394"
     CDS             complement(8417..8692)
                     /locus_tag="ACHKUI_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6600463.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(8686..9330)
                     /locus_tag="ACHKUI_004395"
     CDS             complement(8686..9330)
                     /locus_tag="ACHKUI_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6600464.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            9559..10530
                     /gene="parM"
                     /locus_tag="ACHKUI_004396"
     CDS             9559..10530
                     /gene="parM"
                     /locus_tag="ACHKUI_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO6600465.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            10535..10927
                     /locus_tag="ACHKUI_004397"
     CDS             10535..10927
                     /locus_tag="ACHKUI_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6600466.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRTVLTELGASQ
                     SVEKMPPSATDSVQEAMNDVRLKMKKLF"
     gene            complement(10932..11850)
                     /gene="umuC"
                     /locus_tag="ACHKUI_004398"
                     /pseudo
     CDS             complement(10932..11850)
                     /gene="umuC"
                     /locus_tag="ACHKUI_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
BASE COUNT         3020 a         2663 c         2858 g         3309 t
ORIGIN      
        1 gttgtacaac aaaccaatac caagatgctt ttaatattca tcacatcaaa gaaaatatca
       61 agtcgatcca gacgactgaa atatccccag agaactatca acgcaatccc tgatataata
      121 cttgatggtg ttaatactga aactattagc gtcttccact tctgattcat tttaacttcc
      181 tggttttgct ataaagagag cataaaaaag agtgaataag ttattacact tcattgtttg
      241 actcctcgca gtaacctttc aggaaggttg tcggatattt ttgataaatt atgtcataaa
      301 catattattg taagtaatga tttcactaaa tttatttgat cgtcgtcata atatttttaa
      361 tctgaatata gaagatgatt gtgtcatatg cagtatgata taaaacgtta aaagtgactg
      421 aatagataac agttcatgca tgaacaagac cataacataa cataataaaa tatgcttatt
      481 atgcataacc ttatgttgtg actaagccgc cttaaaactg agaagtagct aagaatatgg
      541 cgtcagacac cttcagttgt cgtagaaggt tatctgcagg atttttctcc gtatctgcct
      601 gttcctggca ttaccccaat tctcccaaaa acggtaaacc agctctcagc ccgcaccatg
      661 actggcttaa gacgggatcc ctgcggcaaa atgcgccttt acgcatttgg ttttacccgc
      721 aataatgcgt gataatcaaa caaaaggatc cgcgcgctgc gatttattgt cgtggaagga
      781 tccgggcgtt caggtcagaa tacctgtatc cataaacagt gcgtgtgcta cgtgaaaaat
      841 aactcatgaa caatgtcatt cccctgcaga attcaccaga acgcgtctcc ctgttaccca
      901 ttgcgccggg ggtggatttt gcaacagcgc tctccctgag aagaatggcc acttccacgg
      961 gggccacacc ggcctacctg ctggccccgg aagtgagtgc ccttcttttc tatatgccgg
     1021 atcagcgtca ccatatgctg ttcgccaccc tctggaatac cggaatgcgt attggcgaag
     1081 cccggatgct gacgccggaa tcatttgacc tggatggagt aagaccgttt gtgcggatcc
     1141 tgtccgaaaa agtgcgtgcg cgacgcggac gcccgccaaa agatgaagtg cgcctggttc
     1201 cgctgacaga tataagctat gtcaggcaga tggaaagctg gatgatcacc acccggcccc
     1261 gccggcggga gccgttatgg ccggtcacgg atgagacaat gcgcaactgg ctgaaacagg
     1321 cggtgaagcg ggctgaagct gacggcgtgc atttttcgat tccggtcaca ccacatacct
     1381 tccggcacag ctatatcatg cacatgctct atcaccgcca gccccggaaa gtcatccagg
     1441 cactggctgg tcacagggat ccacgttcga tggaggttta taccagagtt tttgcgctgg
     1501 atatggctgc cacgctggca gtgcctttca caggtgacgg gcgcgatgct gcagagatcc
     1561 tgcgtacact gcctcccctg aagtgacagc atacgctctt ccttatataa gcggaggtaa
     1621 gcagaacagg cataacataa gctatagcag gtagataaac tgtagtgagt aagccgtgtt
     1681 ttatgtaagg aacaggcaga tggcagctca gccacagaca gtgcagcagg aaactgaata
     1741 taaactacag tgagcacatc actcactgca cctgttaata taaactgtag tcagtaaaga
     1801 gtctctctta tctgactaca gtttatgttc agcgggattt gaagagtttt tccaggtcat
     1861 ccagcgtgat gcccagtttt tcaagcaggg caagcttctc cgccatctcc ggactgattt
     1921 tttccgcagg tgaaggtggc aactgatttt ccttactctc atcattcggc gcttttaacc
     1981 ggggacgccg gtagtgaata cagaagaatt ttgtccgccc ccgctggatc tccgtgtaat
     2041 caagatatcc aatctcgcgc aactgctcca ttgcccgtct gaccgtctgg ttctgggaaa
     2101 atacaggaga cttcagattg aggcgtgcac gcagccgcgc cagcgatatc ggtgccggat
     2161 cccggggcag gctctctata aaggtgtaaa gtgcctgggc ggactcccgt cgcttcaggg
     2221 cattaatcgc cttaagctga agaaggacct ttctgtcaaa ctggtacagt tcaaaaaggc
     2281 ggggatcagc ctgtaactga acaatatccc gttcagtatc gtagtaggct gactgtacca
     2341 gatgggtgat gtattcccgg gtgtgcttct catcggtgcg ggaaaaggag atcacggtac
     2401 cggcaatgcg tttcagggaa gggctgatgc gctcacgcag cctgcgtgat gactggcttg
     2461 agggtatacc acacagtttt gcaaactcga caaaaggcag ttcaactttg tcaccaatca
     2521 cgttatggcg ggcaaaggaa tgaatgatcc ccacccaggt cttgaaatcg ttatccatat
     2581 ccagccgggg gccggtgatc tcaaccttat cgaatccttc tgcacgggcc agagaaagac
     2641 gcgtcagttc ttccgtggca tcggtgcgtg acagtgtatt ttttttactg ttcttcagtg
     2701 acttaagggt aggtacaaag acgcccagac gcatcagcgc cacaggttgt acggtgttgt
     2761 tattgtttgg tgtcagtgtc accagctcac cggacgactt atccacctcg ccgaacagtt
     2821 ttttgatgtc tgaattttcg ttttccagaa agcctcctgc ctgtggacag taaaagaata
     2881 aacagaaaat cgtgtgtcag aaccccgtca actgactgaa gaaaaacctg cagtctgtat
     2941 ttaacaactg agtctggcgt actgaccata gtttatgtca ttaacttact tcagtatatg
     3001 cattactgaa tacagcttat catgaacctg ttacagttta tccataacat gccacagctt
     3061 atgtgaacgt ggtcccggcc catgagtgac aaggtttgca gcgatatggg gatctttaat
     3121 ggatcctttg ttgatcattc atggatctgt ttactggatc gggcctctgg atatgtggat
     3181 aaatcattcc ggaaaaacaa catacactac cgattatgaa ggcttcaggg cgtacctgct
     3241 cactgtaact acagatatat tgcgcttcct cgccggttcg cgggcttcgc ccggtcaccg
     3301 gtctgcggcg agcactgttt tttctgaagt ctgtctttac aggtaatttt tgacggaatc
     3361 tgccgtactt cctgagcgta cagtcatatc cgaaatacac ttcatatgtt tgtctcaacg
     3421 ccagaggcgc atgcagactg gctccatgaa tatcctgaca actggcatca cttaatttac
     3481 cgacagtctt aagactaatc gtcagaatcc tgataattga cgtttcaggt ccagagaagt
     3541 gcagacgttg caaccagtca gctgtgcctg tgtcggagct gatatccgtg atgaagcaaa
     3601 ccaaagagct gtagcatcag ttgggcaaaa cgagctactg taaaaagccg ttggaccgac
     3661 aaaataagtg aatatcgtat atgcctttgc tgccggagga tgacagaata aaccctgtca
     3721 gttattgact ctggatctta cgagcccaac tgtatgccat aatctgtcgg tcgaaagagt
     3781 gacaggatcc tgatgacagg cactgctgcg cagacaccgg tcatggatgt aatggaaata
     3841 ccagctgaac cgcaggtctt ccagtcgggg acacagagta cagggctgga gcttgtggat
     3901 acgtatctct ggatcttcaa acactttatt gaaggaaagg agttaaccag atcccttgcc
     3961 tgccaggttt atatcaagcc ctatagcgac aggatggaca gtgtcacctt ccagtcagta
     4021 gctaaacgat ctaaggtgtt cctgaacaaa ctgcaggagc caacgtctga aatgataaat
     4081 aagcgaggga atacctggtg ctggaagagt tgagatgact ggcacattgg tataaatctt
     4141 gccgtcattc tgatcagctt gtaacattct gtaatgatca ccattggctg gcgatttttc
     4201 tgttcagtaa tgtaattaac cttatctgat gggctggcca ctattccatc agctgtactg
     4261 atggcaggct ccctgttgag gtctgccgcg agctgtccgc cggactcaac gggctctgcc
     4321 acaatccggt tatgatagcc tggaaactga tcaagggtta ggtcgatata gtccctggca
     4381 gggtcggcaa tgacgtggcc gtatctgcca ggaccattat cccactgcat accgacaata
     4441 caggagtaca ggttgctgcc gtcttccgtc agataatcag tcagcgtctg gctggccagc
     4501 tcgctgcaac cggccgggaa atccttcagg tgatcatacc cggcggtgag agaagcctca
     4561 caggaagtca gctccagagc atgtcggaag gcctgcaggc gggtccaggt gtccgcattc
     4621 actttttcgc cttcccgtca tgacgggcca gggactgaag tttacggacg tcctcccggg
     4681 atcctttcag ctcagctttc gtatccagca ggtgcgccgc cagcggtctt tttcccggga
     4741 gccgactttc ctggcacata ttcttttcgc agttatcccc agaatctgtg gataactgtt
     4801 gctcctcttt tgagagagaa ggctttactc accgcctgaa tgcggcagat atgtctggac
     4861 gatatatctg catacgccgg tactgcttcg tcagattgca cggcaggtga attgccgggc
     4921 tggtaccata ccatatttgc gccgaacccg tccggggcgg tgccgataac agatgttaaa
     4981 tgattggtcg tttttacatc tgtaaaagca acagggcccc atgattatat catggggccc
     5041 tgttactctg attcttcgca atagattgct ctgaattaat tggtgtttat ctgtattttt
     5101 tgttttcgga acgtttttgt aagaattatc ttttatattt gaggtgatat cttattgtgg
     5161 ttgttgtaat agtttagagt cgtaataaat atgattgatc gccactttaa atgaagttaa
     5221 tctactggag atgaagtaac ttcattaatg cgtcataaaa ataaaaattc ctgttttggg
     5281 aaatatgaat gtgtctgtcc cctccttcgg gagggtgttt tttatctgga aactggtttc
     5341 tggttctgat ttttcttttt gtattatagc gagttacgca tatttaccaa tcgatgtctg
     5401 gcaaacagag tatggcgatg gtgtgatttg tgtttattgg taaaaaaaac acccccgtag
     5461 tgggggtgtt gagacttcaa cctatgaact cattatcctg ggatggatga gtagcgtgcg
     5521 atattgaatc atattcagca ggaataataa tattcgcaaa aaaatggatg tgcaaggaaa
     5581 ttttttatat gcttttgtgt gtggtggtta ggtaatcaag aagataaatg atgaagtgtg
     5641 ccagttcagg atcaatgttt ccatgggaaa gccatttatt aataatatca attaagttcg
     5701 aaagctcagg gaagatttgt tcaatgtttt cctctgtctc ccgcacagtc atatagcgac
     5761 gtctccttgg ggattgaaaa caaaaacaat aatacaacaa gtatgtaaca gaaggtaggt
     5821 ggcaggggcg gaacaggaaa acgcatgagg gcgcttcctg ggggagatgc ctggtatctt
     5881 tagggttcag ttttgagatc gggaattttt gtacattaag tgcattattt aatcaattga
     5941 aacgcgtaat ggattgattt tatcagcaac tatcgcgttt aagttgtcga tcatatttga
     6001 gttgtctcat taaggtcgaa tcccctgaaa gcggcgtata acgtaggagt tcccctctat
     6061 agcaataggt aaggctgttc aaaaaaggct taccgtacaa taattcgcta ggcccccctt
     6121 gtcatacaag ctcgtaagcg gccctgacgg cctgtcaatg cggtactgcc ctgtcgggac
     6181 tgctctaccc acataagttt ctacatagct tgaagctgga tggggaggta ttacggcttt
     6241 agccgtaata ctttcttcgt cctcgcagcg ctgacagttt tcagggatca aatttaggtc
     6301 cgccatacac tccgtaaaga ctctgtcagt tcacaggtat tacctagtga agacgtatca
     6361 taaaatgaaa actgtagttg ggtagttgct ccccctcacg gcatgcaggg gctgaggggg
     6421 aaaatttcct tatcttgttg ttgctatttc tggcttagtc agttttgaat tttgcttcct
     6481 aagcaataat cccctcaccg caacaatcgt tcttctacac taataagatc cgaaaaaagg
     6541 tagtcacgga tcttcctctc ccgccgcaca gcgccatctc ccgtcagcac aacatgtagt
     6601 gctgaattta gctgttaatg acactatatg ttgtgccatc tccctgacct gtgatgcgcc
     6661 gcgcaggggc ggaaaacagt gatatgatga ttttctcagc gtggtacact tccggaaagt
     6721 tgtgatatta atgaaagtcg gatctgacgg aaacgggaat gcgataatta cggtttgttt
     6781 ttataagcca gattttgatt tttattggtt aacataacgc aaggtaaaat aaataaggct
     6841 tactaaaagt aagataacag tatgtgtatt tgcgcgctga tttttgcatc ataataatat
     6901 atacttatat gcatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     6961 agtgacagtt gacagcgaca gctatcaatt gctcaaggca tatgatgtca atatctccgg
     7021 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     7081 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     7141 cgatgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     7201 gttatcgtct gtttgtggat gttcagagtg acattattga cacgcccggg cgacggatgg
     7261 tgatccccct ggccagtgcc cgcctgctgt cagacaaagt ctcccgtgag ctttacccgg
     7321 tggtgcatgt cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     7381 tctccgttat cggggaagaa gtggctgatc tcagtcaccg cgaaaatgac atcaaaaacg
     7441 ccattaacct gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg
     7501 cagccagggt accggcagtt tattctgccg gacagtctcc acaacttcct gttgtcattg
     7561 attatccggc agctctggca cttcgccaga tgtcgatggt tcatgatgaa ctgccgaaat
     7621 atctgctggc tccggaagtg agtgccctgc tccattacgt cccggatctg cgccgcaaga
     7681 tgctgctggc cacactgtgg aacaccggtg cgcgcattaa tgaagcactg gcgctgacgc
     7741 ggggggattt ttcgctcgcg cctccgtatc cgtttgtgca gctggccact ctgaagcagc
     7801 ggaccgaaaa agccgccagg acggcaggaa gaacgcctgc cggacagcag actcaccggc
     7861 tggttccgct ctccgactcc tggtacgtca gtcagctgca gacgatggtg gcaacactga
     7921 aaattcctct ggaacggcgt aataaacgaa caggcaggac agagaaagcg cggatctggg
     7981 aagtgacgga cagaacggtc aggacctgga ttggggaggc ggttgccgcc gctgctactg
     8041 acggtgtgac gttctctgtc ccggtcacgc cacatacgtt ccgccattcc tatgcgatgc
     8101 acatgctgta tgccggtata ccactgaagg tcctgcagag cctgatgggg cataagtcca
     8161 tcagctcaac ggaggtctac acgaaggtgt ttgcgctgga tgtggctgca cggcaccggg
     8221 tgcagttttc gatgcctgag tccgatgcgg tcacaatgct gaaaaacaga cacgcataat
     8281 tcataaatca taattatgaa ttgtgattta ttctgtaaaa aaagagacca ctgcaatatg
     8341 tgatctcttg tatgcaaggg tgcttaaaca gtatgaattc acaagtgtga tttcataagt
     8401 aataacttat tgattattac gttagtgttt ttaagtattg ctcaagggct actctgacga
     8461 tggcacttac attttttgga tctttaccat tgcgtttatt cctcaatgcc agatcttcta
     8521 ttgtgacaag catgctttca ccaagagaga ttgtcgtccg acattgtttt tctggttcgg
     8581 gtttttccgg cgcgccgtat ggtttatccg caaggcgctg agccagagcc tctgcttgtt
     8641 ctaccgtaac tgcagctctg tttaatgctt gctggctggg tttcttcacc atgggccaaa
     8701 tacctcattc attaaatgtt caatttctgc tttggctgct gtattgttcg tttctactac
     8761 accggtgccg ttgctcatgc aatcgcgata gacttttctg aaacaaataa ctgaatcaag
     8821 gacttgaatt gttgggaatt cttcaagata ttcaaggaat tcttttctct catttcccct
     8881 caaaaccgga ttggttgttg ccatactttg taaacaataa accttaagtt ccgggttaag
     8941 gttacgcatt gcgtctatct gttgttctag ttctgtaaga gtatccagat ctggttgaga
     9001 acactgtagt ggcgcaatta tttgatgtgc gacaactcca ctcgttatga attcctttga
     9061 gtttcttcct gcgacatcca ctattacatc atcgaacttt tcattcagag cctgtagtgt
     9121 ttgtgtcagg ttatcaaatt tctcaacaag ggttattgct ggtaacagtc ctgcagcttc
     9181 tcgttccgca tgatgttttg cggctgttcg ttgtagatct ccgtttaaca ggcagacttc
     9241 ccttccttta tttgcgagag caatagccag gtttgatgca atggtagttt tccctgaacc
     9301 acctttgttc ccaccaacta cagtaatcat tgcaacctca taaatgtgat gtgtgaagta
     9361 tgatgatatt ttgacacggt aacctgaggt gtcaatatgt gttcatattt atgaagtatg
     9421 aaatgtgaag tgtgccatgt gatgtaataa ataatacttg tgttttgtga tttcataact
     9481 atgatggtgc tcaaattgag tattaccttg caaccagaaa caatgattta ctcagattga
     9541 gtatttaagg ggctatagat gaacgtatac tgcgatgatg gttcaacaac aatcaagttg
     9601 gcatggaatg ataatgggaa aatctgtaag tcgttgtcgc aaaactcatt tcgtcatgga
     9661 tggaaggttg acgggttagg aattcgccag acatttaact atgaactgga tggtaaaaaa
     9721 tacacgtatg atgaagttag caaccaatct attcttacga ctcatattga gtatcaatat
     9781 acggatgtta acctgttagc tgtgcatcac gctttactga atagtggatt agcgcctcag
     9841 cctgtatcat tgactgtgac tttgcctatc agtgagttct atacgaaaga gtgtcaaaaa
     9901 aatgaactca acattcagcg taaaattgag aatttaatga ggccgattcg tcttaataaa
     9961 ggcgatgttt tcaccattga acacgttgat gtgatgcctg aatcattgcc agctgtgttt
    10021 tctcgtctgg tcgtggataa agttggtcag ttcgaaaaat cgctggtagt tgatattggc
    10081 ggcactacgc ttgatgttgg tgttatcgtt ggtcaatttg actctgtaag tgccattcat
    10141 ggtaattctg gcattggtgt gtcttcagtt acgaaagctg caatgagtgc attgcgcatg
    10201 gcatccagtg ataccagttt cctcgttgca gatgaattga ttaaacgtcg taatgacccg
    10261 gactttgttc ggcaggttat aaatgacgaa acaaaaactg atctggtcct taataccatt
    10321 gagggggcta ttgccagttt gggggagcaa gtagtcaatg agctcggtga tttccatcat
    10381 gtgaatcgtg tttatgttgt cggtggtggg gccccgttga tctatgattc gataaaaacg
    10441 gcatggcacc atcttgggca aaaagtagtg atgatggagt cgccacagac agcactggtt
    10501 gaagctatcg cggctttcaa agaggagtaa tattgtggac gatgaacgga aaaggaaaaa
    10561 atacacactt tatctgcatc cggaaaaagc tgcagacttt caaactttgg aagcgataga
    10621 gtccgttccg cgttctgagc gtggtgagct gttccggaat gcatttattt ctggaatggc
    10681 gttacaccaa cttgatcctc gcctgcctgt attgctgaca gccattttga gcgaggagtt
    10741 ttcagcagat caggttgtaa ccttactcag tcagacgact ggatggaagc cttctcaggc
    10801 cgatatcaga actgtgttga ctgaactcgg tgcttcgcaa tctgttgaaa aaatgcctcc
    10861 ttctgctact gattcggtgc aggaggcaat gaatgatgtg cgtcttaaaa tgaaaaaatt
    10921 attctgatat atcagaatga agcaatgggg atctcagtcc attgtgttgt atacgaaggc
    10981 gatagcattt cccgtttcat ttgccattca ggggcggttc ctcgccccgc aaaccacact
    11041 ttcccttttc ccgactggtt cagttcatcg agtgttttca ttaacttttc actgttttta
    11101 cggggctgga tttcatcaaa taatcccggc tgcgctatac ccgatggtgt gaaatcagcc
    11161 agcatgactc ctgccttcat atagcggtac ccttcacgcc agacatggtt taagactctg
    11221 catgcggcgg caataatgtc ccggctgtcc tgtgtggcaa tgaaagcttt tccacagcgg
    11281 cattgctgta acagggttct tttactgcaa agggggatgt ccgtacaaat gtcgtcacct
    11341 gccgacaata ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca tactgaacaa
    11401 cagcctggtg catggcatct ttgtctgtga ttcgttcacc aaaactgcga ctacagacaa
    11461 tctgctgttt tgccggtggt acttcttcca gggatatgct ggactcgccg ttgagttcgc
    11521 gtaccgtacg ctcaagaatg acgctgaagt ttttccggat gaatgccgtg ttagcctgcg
    11581 ccagttgcag tgctgtgtta atacccagcg cattcagctt ttccgtcagt ctgcgtccta
    11641 ctccccagac ctcaccaact ggctgcagcc ccagtagctt caagatccga ttacggtttt
    11701 ctgccgtcag cgcgaccaca ccggaaaact gtggccattg ctttgttgcc cactgtgcac
    11761 ttttagccag cgtttttgta ggcgcaatgc ccacccccat ggtgagtcct gtccagctct
    11821 ttacctgttc cctgagctga tgaccaaaaa
//
LOCUS       DAZFRE010000132        11841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFRE010000132 DAZFRE010000000
VERSION     DAZFRE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.132"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            87..1865
                     /locus_tag="ACHKUI_004399"
     CDS             87..1865
                     /locus_tag="ACHKUI_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6600467.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1905..2384)
                     /gene="xdhC"
                     /locus_tag="ACHKUI_004400"
     CDS             complement(1905..2384)
                     /gene="xdhC"
                     /locus_tag="ACHKUI_004400"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6600468.1"
                     /translation="MNHSETITIECVINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2381..3259)
                     /gene="xdhB"
                     /locus_tag="ACHKUI_004401"
     CDS             complement(2381..3259)
                     /gene="xdhB"
                     /locus_tag="ACHKUI_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6600469.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGSDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3270..5567)
                     /gene="xdhA"
                     /locus_tag="ACHKUI_004402"
     CDS             complement(3270..5567)
                     /gene="xdhA"
                     /locus_tag="ACHKUI_004402"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6600470.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5981..6736
                     /gene="actS"
                     /locus_tag="ACHKUI_004403"
     CDS             5981..6736
                     /gene="actS"
                     /locus_tag="ACHKUI_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6600471.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6815..6888
                     /locus_tag="ACHKUI_004404"
     tRNA            6815..6888
                     /locus_tag="ACHKUI_004404"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6847..6849,aa:Gly,seq:ccc)
     gene            complement(7204..8433)
                     /locus_tag="ACHKUI_004405"
     CDS             complement(7204..8433)
                     /locus_tag="ACHKUI_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6600472.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            8688..9869
                     /locus_tag="ACHKUI_004406"
     CDS             8688..9869
                     /locus_tag="ACHKUI_004406"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6600473.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            10156..10992
                     /gene="kduI"
                     /locus_tag="ACHKUI_004407"
     CDS             10156..10992
                     /gene="kduI"
                     /locus_tag="ACHKUI_004407"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6600474.1"
                     /translation="MDVRQSIHSTHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            11022..11783
                     /gene="kduD"
                     /locus_tag="ACHKUI_004408"
     CDS             11022..11783
                     /gene="kduD"
                     /locus_tag="ACHKUI_004408"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6600475.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         3029 a         2739 c         3002 g         3071 t
ORIGIN      
        1 atttcgcctg agttaatatc atcatgatct gtgaaccatc aacgtcttca atgatcgact
       61 tttaagtaag tctggaaggt aaagctatgg agcttgcgac tacgcagtca gtattgatgc
      121 aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg cagcttgagg
      181 ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac gggcgcgtat gggaagtttc
      241 ttggccgcca gttgagcggc aactcacgcc tgctccgcca cgtcctggaa acgaaaactg
      301 aaaaagttgt gacacagtct cgcttcgatc ccctttgtga aggttgcgat agtaaagaaa
      361 attgccgcga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt tgtgttgggg
      421 tgataagttt gattgccgtt acccacgagc aacaagaaca tatcagtgat aatttacgcg
      481 aattttccga ttacgttcgc catatatcca ccatttttgt ttctaaactt ctggaggatc
      541 agggaccagg agataacatc agtaaaatat tcgcgaccat gatcgataat atggatcagg
      601 gcgtgttagt ggttgatgct gataatcgtg ttcagtttgt taatcagaca gctttaaaaa
      661 cactcggtgt tgtacaaaat aatattattg gaaaacctgt tcgtttcaga ccattaacat
      721 ttgagagtaa ttttacccat ggtcatatgc aacatattgt ttcgtgggat gataaaagtg
      781 aattaatcat tggtcagttg cataacatcc agggccgaca actattttta atggcgtttc
      841 accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccgcat attgaacaat
      901 tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt attgcaccca
      961 gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtc gtcgctcgtg
     1021 caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac tgtgccgcga
     1081 ttccggaaca gcttctggag agcgaactgt tcggttatgt taaaggcgca tttactggcg
     1141 cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg ctgtttctcg
     1201 atgaaattgg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct attgaggcgc
     1261 gtgaaattct gccgattggt gccagtagcc caatacaagt cgacatccgc atcatttctg
     1321 caactaatca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa gatctcttct
     1381 accgacttaa tgttatcccg ataactcttc caccgctgcg tgaacgtcag gaagatattg
     1441 aattactggt gcattacttt ttacatctgc atacccgtcg tctgggatcg gtttatcctg
     1501 gcattgctcc tgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga aacctgcgcg
     1561 agttaagcaa tttgatggaa tatctggtta acgttgttcc ttcaggtgaa gttatcgaca
     1621 gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt gatgtaacag
     1681 aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg gaggagatgg
     1741 aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaaggaa gttgctgatg
     1801 aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag ttgttaaaca
     1861 cataacctct gagaatccgg cctgaattca ggccggatat ccttttactt cgttttctcg
     1921 caatccagaa ctgtatttac aatcatctga taccccgtgc agcgacaaag atttcccgcc
     1981 agtccacgac gaatttccgt aatggttaat ggtttttcgc gtggttttgc cagcatcgcc
     2041 gtggtagcca taatcaggcc cggcgtacaa aacccgcatt gcactgcgcc ggatttcgca
     2101 taagccagtt ggacatgaga aagtttaccg cctttcgctt caccttccag cgtgcggatc
     2161 tcttttcctt cagcccaggc cgcaaggaat aagcaactgt ctatcgcagt gccgtcgacc
     2221 agcaccgtac aggcaccgca ttcgcctacg cagcaacctt gtttgacact aagaagccct
     2281 tgttctcgga gtagttccga aagcggcatt cctggcgcgg cgtgaagctg aaaaggcatc
     2341 ccgttaatga cgcattcgat ggtaattgtt tcgctgtgat tcattgcaat tttccccccg
     2401 ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa
     2461 actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggact gattcgctga
     2521 tggcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc
     2581 agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt
     2641 tatccagtcg gcaatgtgcg gcgcagccaa ttgttgaaat atccattgcg tcgcgcatgg
     2701 catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga
     2761 caaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg
     2821 ggacgaaacg aacaccgcgc ggggagtgga tctcaagctt cgcgtcataa attagcgttg
     2881 gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt
     2941 tacggatctg cggtccggca atggatgagg ccgcagcaca taacgccggg agatgacgtt
     3001 gagttacagg atcttcaatg agctgagtaa atgttgtcgc ggagccgatt cgcagcgcgc
     3061 catcttccgc ctgtgttatt ccctgaagct ctgccagatt gtggatatca acaatatggc
     3121 gatagcggtc attgtgatgg tgaagctgta tcagtacgtc agagccaccg gcaagcaatt
     3181 tggcctgcgg gttgtcagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt
     3241 aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg gaattcttca
     3301 tataatcgtt ttggcgttag cggcagtgta ttgattgcaa caccggtagc catcttcacc
     3361 gcgttacgaa tagcagcggc tacaggaatt atgggtggct cacccagtga cttatgtccg
     3421 tatgcggatt gcggttcatt gatttcgaca aacgcgcttt ccagttgtgg cagatccggc
     3481 atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct tttcgcatcg
     3541 atgatcatct cttcaaatag cgcccagcca atgcccatcc ccattccgcc gtgtacctga
     3601 ccttctgcta gcagcggatt aagaatatgg cccgaatcat gaacgttgag gatgcggttg
     3661 atggtgactt tacacagtgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc
     3721 ggtgggttag tggtggtttt gatggagctt tcggcagaga gctgcccgcc gcgttcaggg
     3781 tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca
     3841 accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg tagcatgact
     3901 gcggcgtgag cgatgatttt ctcttttaat aacagtgccg cactgcgcag cgcaggcgcg
     3961 gcaacatagc tctgacgtga ggcaaatgcg ccggggtcga atggtgtaac gtcggtgtct
     4021 tgtgtcgaaa ttacgcggac atcgctgacc ggaactccca cggtttctgc caccatttgc
     4081 gagaacacgg tgtcggcacc ctggccgatt tccgtcgcgc cgctttgcac gttgatggtt
     4141 ccatcctgat tcatcaacag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta
     4201 gaggtgtagc taaaacaggc gacgccaacg ccacgacgta aattgccttg ctggttctgg
     4261 cactctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc
     4321 aaccctgcgc tgtaaatacg ttttcccgtg agcggattag catctccttc gcgggcggcg
     4381 ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcatc atcaagcata
     4441 gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg
     4501 aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg
     4561 actttattcc ccccagcaga agcaatggag tgcccgtgag atgcataagc gccggtgtta
     4621 gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc catttgcccg
     4681 tcaatagtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa
     4741 actttcaccg gaatgccgcc aagtttgctg gtcaggaatg ccgccattgg ctcttccagt
     4801 acatcctgtt tattaccaaa accgccaccg ataaacggtt tgatgactcg tacgcatgac
     4861 cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg
     4921 gaaacgatgg taattcgcga gtcatcctcc atccatgcca gcgatgtcac gctttccata
     4981 tgacaatgtt gaataacggg agtctgatag tgcccctgta cctggtagtc ggcggcatcg
     5041 attgtttgtt ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt gccaccgtta
     5101 tggattggtg cagcgtcttc tgccagcgcc gcttctggcg aggtgataac gggtaattct
     5161 tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg
     5221 acgatggcaa cggcgtcgcc atgatgacga acatggcgag ttagcagtgc gcgatcggcg
     5281 gtatcgcgct tgttttcgtc aagtgtccag gcatgccctg ccgtggcgaa tgggatttct
     5341 ggcacatctt cccaggtaaa aatcgccagg acgcccggca aactcctggc ttgttcatca
     5401 ttaatactta cggcataacc atgtgcgata ggactacgta catatttcgc gtaacacatg
     5461 cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg
     5521 acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga aatcccctcg
     5581 gtttgaacat ttggttctta tggcaaagat ttagtcttag caggcaagaa ttacgccaga
     5641 aacgactata tgcgtgaggg gaggaaggaa gtgtgatgac gttaaaaaaa cacagatatg
     5701 atacgaaaca ggactctgaa tagggacagt gatacgtatc aatatgagtg gcgataaaat
     5761 caatgtgata aattaggatt attacgcatc aaggtgggaa atagggtaaa agtcaggaac
     5821 ttcgatacca ctacagcaaa tattttgctg atgatacttc cctttgccaa atgaaattaa
     5881 cctctttctc tattacatcg cttttccaga agcaagagat tgggtatcca gaagccggct
     5941 gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg gacgcctgaa
     6001 taaaaaatct ctgggtatcg tgatgttgtt atcggttgga ctgcttttgg cgggctgttc
     6061 gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg tgaaacgggg
     6121 ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagaac tggcgcgact
     6181 gaacggcatt tccccccctt acaccattga agtaggtcag aaactaaaac tgggtggggc
     6241 gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgaccaaaa ccgcatcggt
     6301 tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac gttgttggtt
     6361 atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg gcaataaagg
     6421 gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag gaaaggtggt
     6481 gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac acagtgaaga
     6541 ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc aaagcgtgaa
     6601 ggctgggcaa aaaatcgcca ctatggggag cactgatgcg gcatctgttc gcctgcattt
     6661 ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc ctcagggcag
     6721 caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgtggtact tgctaaggag
     6781 agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat ggtagaacga
     6841 gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccaat ttatcttcac
     6901 atcatctacc tgccattttt gttgtatctg attttctata ttatcttaat tcaaggcttc
     6961 agacttttcg caatcgccgt aatacttccc catttttttg taaataaaaa gcgtttacgc
     7021 aatgcaatcg tcatgtatct taaatagtct tatttaagat gttcagaact ctttgtgtgt
     7081 ttaactatct gataattata taatattacg agagaagtga tagagcggaa taataacaaa
     7141 tgaacattat gaaaactggg atggttcaaa attgacgcaa ttttgaacca tccacaacgg
     7201 atattagcta aagaacatta ccgatacgca cagaataccc acgatcagcg taatcagatt
     7261 tccgatggaa cgccagggtt taagcgccgg gatgagatac gttgacagcg taggcatgat
     7321 gaaaagtatc atggcaatga gcgggccgct gatcgcgtaa atcatcgaaa tcgcgttcgg
     7381 gttaatgcaa caaacaatga aggtaatcag cgataccaac ataattgata gtgcgcggtt
     7441 aaatgcacga cttttcttta caccaacctg ctgtaatgtt gttttgacga cctctgtggc
     7501 accttcaata acgccaaagt acgtacccaa gaatgatttc gacatcgcaa ctactgcgac
     7561 aataatcccg gaaattgaca accatgctgg ggcgttcggc agcatagaaa gcgccgataa
     7621 aatggtaacc ccttcttctt tagccgcttc aatatacgaa ggtggaatag aaagcaggca
     7681 gctaaagaca aagaacaata cacttatgca gatgatgaga taagcgactt tcataatttt
     7741 tttgcattta tccatagcat gttcgccata tttttcacgt ctgtctatgg caaacgtaga
     7801 aataatgggc gtatggctaa aagcgaaaac catcacggga atcgatatcc atatctggtg
     7861 aagggtattc tgattgaact ctacctgggt tgttaataga tcaggttgcc aactaccgac
     7921 caggtaaata gaaagaaata agaaataggc aatcaatggg aataccaaaa atcccattac
     7981 ccgaatggtt gcatggcgtc ccatcagaaa aatgagattc aggattaata caacccccag
     8041 actcaccaac atacggatgc gaagatcaat aaccatatgc tttgccagtt gttccgtaag
     8101 tgagttggta attgccactg catatatcaa cacgacgaca aaaaaggcga tgaagtacag
     8161 cgtggtaatc agattaccaa tcttcttgcc atagtagtgt gttaccgcgc ccgttatccc
     8221 ttcacctgct gatgttttcg atgagaggat gaactggcat aaggctttat gtggccaata
     8281 tgttaaaggc caggcgacca gagcagtaat aaacaggacc acagcccccg ccgaacctaa
     8341 ctgaatagga aggaagagcg tgcctgcacc aacggctgtg ccgtagagcg cgaaactcca
     8401 cagagtttct tcttttgacc aaatattaga cattatctga acgtatcatc tacaaattaa
     8461 acaaaatgga gggcaattta ccataaacca ggttaagtat gggagattaa cgccttatct
     8521 acctctctat tggtgggtta gtggttgcaa accttacgtg ccagttatgt ctctgactac
     8581 tactcaggcg tatttccttt gtgataatag tcactttttt taaacaatta agataactgt
     8641 aattaacaat catttactat tgcactgtta attggtgcga ggttgtgatg aaagacgttg
     8701 tgattgtcgg ggcgttacgg acacctatcg gctgctttcg tggtgcgtta gcgggtcatt
     8761 ccgccgtgga acttggcagc ctggtcgtca aagcgttaat agaacgtacc ggcgttcctg
     8821 catatgcggt ggatgaagtg attcttggtc aggtgttgac tgcaggggca gggcagaatc
     8881 cggcaagaca atcggctatt aaaggtggtc ttcctaatag cgtttctgca atcactatta
     8941 atgacgtttg tggttccggg cttaaagcac tgcatctggc tactcaggcg atacagtgtg
     9001 gcgaggctga tattgtcatc gccggtggcc aggaaaacat gagccgcgca ccacatgttc
     9061 tgactgatag ccgcaccggc gcacagcttg gcaatagcca gttggttgat agtcttgtgc
     9121 atgatgggtt gtgggatgcc ttcaatgatt atcatattgg tgtcaccgcc gaaaatctgg
     9181 ctcgcgaata tggcatcagc cgtcagttgc aggatgctta cgcacttagc tcgcaacaaa
     9241 aagcgcgagc ggcgattgac gccggacgat ttaaagatga gatcgtcccg gtaataaccc
     9301 aaagtaacgg tcagacgttg gttgttgata ccgatgaaca gccacgcact gacaccagcg
     9361 cagaagcctt agcccgttta aatccttcat ttgatagtct cggttctgtg acagcgggta
     9421 atgcatcatc cataaacgat ggggcagctg cagtaatgat gatgagcgaa gccaaagcac
     9481 gagcgttgaa tttacccgtg ctggcccgca tccgcgcatt tgccagcgtt ggtgtggatc
     9541 cggcattgat gggaattgcg ccggtgtatg cgacccgccg ttgcctggag cgtgttggct
     9601 ggcagttggc tgatgtcgat cttatcgagg ctaatgaagc gtttgctgca caggcgcttt
     9661 cggttggcaa gatgcttgaa tgggatgagc gtcgggtcaa tgtcaatggt ggtgcgatcg
     9721 cactcggtca cccgataggc gcttccggtt gccgaatcct ggtttctctt gttcatgaaa
     9781 tggtgaaacg taatgcccgc aaaggactgg caacgctttg tatcggcgga ggccagggtg
     9841 tggcattgac cattgaacgt gacgagtagc tctgcatttc ctccttaccg ataaatatct
     9901 gctgtcatta atctgttgaa taatgatggc tggtgagtcg acataccttt tcagggctcc
     9961 ctgatttttc cattctcatg acatattcgt gatcgacact gcactttttt taatccttaa
    10021 cttgaaaatg aaacattgtt ttatttttaa ttgaaaaccc tatcccatgg gcatatcatc
    10081 ccctcataga gaaaacgaaa cagtgtttca ttgttgtttt ctattttcac gcttcactga
    10141 ttatcggagg ttgatgtgga cgtaagacag agcatccaca gtacgcacgc aaaaacgctg
    10201 gatacccagg ggctgcgcaa tgaatttttg gttgaaaagg tatttgtcgc cgatgagtac
    10261 accatggttt acagccacat tgaccgtatt attgttggcg gcattatgcc ggtaactaaa
    10321 acggtttccg ttggcgggga agttggtaaa caactcggcg taagctattt ccttgaacgt
    10381 cgcgagttag gtgttatcaa tattggcggt gccggtacga ttactgtcga tggtcaatgc
    10441 tatgaaatcg gtcaccgcga cgccctgtat gttggtaaag gtgcaaaaga agttgtcttt
    10501 gccagtattg ataccgccac tccggcgaag ttttattaca attgcgcacc cgcgcatacg
    10561 acgtatccca ccaaaaaagt cacaccggac gaagtatctc cagtcacgtt aggcgataac
    10621 ctcaccagta accgtcgcac gattaacaaa tattttgtcc cggatgtgct ggaaacctgc
    10681 caattgagta tggggctgac ggagctggct ccaggtaact tgtggaacac catgccgtgt
    10741 cacacccacg agcgccggat ggaagtttat ttctatttca atatggatga tgacgcctgc
    10801 gttttccaca tgatggggca accgcaagaa acgcgtcaca ttgtgatgca taacgagcag
    10861 gcggtgatct ccccgagctg gtcgatccat tccggcgtcg gaaccaaagc ttataccttt
    10921 atctggggca tggtcggtga aaaccaggtt tttgatgata tggaccatgt ggccgttaaa
    10981 gatttgcgct agttgtgggc ataaacgaat aaggtattgt tatgatttta agtgcatttt
    11041 ctctcgaagg taaagttgcg gtcgtcactg gttgtgatac tgggctgggc caggggatgg
    11101 cgttggggct ggcgcaagcg ggctgtgaca ttgttggcat taacatcgtt gaaccgactg
    11161 aaaccatcaa gcaggtcacg gcgctggggc gtcgtttttt aagcctgacc gccgatctgc
    11221 gaaagattga tggcattcca gcactgctgg atcgcgcggt agctgagttt ggtcatattg
    11281 atatcctggt gaataacgcc ggattgattc gccgcgaaga cgctctcgaa ttcagcgaaa
    11341 aagactggga cgatgtcatg aacctgaata tcaagagcgt attcttcatg tctcaggcag
    11401 cggcgaaaca ctttatcgcg caaggcaatg gcggcaagat tatcaatatc gcgtcaatgc
    11461 tctccttcca gggcgggatc cgtgtgcctt cttataccgc atcaaaaagc ggtgtgatgg
    11521 gcgtgacgcg attgatggcg aacgaatggg ctaaacacaa tattaatgtt aatgcgatag
    11581 ctccgggtta catggcgacc aacaatactc aacaactgcg ggcagatgaa caacgtagcg
    11641 cggaaattct cgaccgcatt ccagctggcc gttggggact gccgagtgac ctgatggggc
    11701 cggtagtgtt tcttgcctcc agcgcttcag attatgtaaa tggttatacc attgctgtgg
    11761 atggcggttg gctggcgcgt taattcattc ctcttacttt tatgaccctg ccgcatggca
    11821 gggtttttta tatctgtaga t
//
LOCUS       DAZFRE010000133        11403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFRE010000133 DAZFRE010000000
VERSION     DAZFRE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.133"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            252..551
                     /gene="yoaC"
                     /locus_tag="ACHKUI_004409"
     CDS             252..551
                     /gene="yoaC"
                     /locus_tag="ACHKUI_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6600476.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHKUI_004410"
     CDS             complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHKUI_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6600477.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            924..2285
                     /gene="pabB"
                     /locus_tag="ACHKUI_004411"
     CDS             924..2285
                     /gene="pabB"
                     /locus_tag="ACHKUI_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6600478.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2289..2867
                     /gene="nudL"
                     /locus_tag="ACHKUI_004412"
     CDS             2289..2867
                     /gene="nudL"
                     /locus_tag="ACHKUI_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6600479.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3051..4415
                     /gene="sdaA"
                     /locus_tag="ACHKUI_004413"
     CDS             3051..4415
                     /gene="sdaA"
                     /locus_tag="ACHKUI_004413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6600480.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4546..6144
                     /gene="pdeD"
                     /locus_tag="ACHKUI_004414"
     CDS             4546..6144
                     /gene="pdeD"
                     /locus_tag="ACHKUI_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6600481.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6148..7704)
                     /gene="yoaE"
                     /locus_tag="ACHKUI_004415"
     CDS             complement(6148..7704)
                     /gene="yoaE"
                     /locus_tag="ACHKUI_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6600482.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7692..7850)
                     /gene="yoaL"
                     /locus_tag="ACHKUI_004416"
     CDS             complement(7692..7850)
                     /gene="yoaL"
                     /locus_tag="ACHKUI_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6600483.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8167..9138
                     /gene="manX"
                     /locus_tag="ACHKUI_004417"
     CDS             8167..9138
                     /gene="manX"
                     /locus_tag="ACHKUI_004417"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6600484.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9201..10001
                     /gene="manY"
                     /locus_tag="ACHKUI_004418"
     CDS             9201..10001
                     /gene="manY"
                     /locus_tag="ACHKUI_004418"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6600485.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10014..10865
                     /gene="manZ"
                     /locus_tag="ACHKUI_004419"
     CDS             10014..10865
                     /gene="manZ"
                     /locus_tag="ACHKUI_004419"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6600486.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10920..11378
                     /gene="yobD"
                     /locus_tag="ACHKUI_004420"
     CDS             10920..11378
                     /gene="yobD"
                     /locus_tag="ACHKUI_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6600487.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMHRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2787 a         2883 c         2867 g         2866 t
ORIGIN      
        1 tgccgttatg ttcgcccttc gattagcatc tctggttttc tttcttacct gaacaatctg
       61 gttatagcgt gaggactgac cattaatttt ctccatctta ctggataaca cactatgagc
      121 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc
      181 tttgatatta gatacaaatt aaggtcatat atagccttat cgataacatg gttaatttta
      241 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
      301 tatcagcgcc aacaccaagt tcgatgaatg aaagtacggc gaagggaatc tttaaatatt
      361 taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag
      421 gctggaaccc tggtttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg
      481 aacgctctgt gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
      541 cactggtttg agttaaattt acatcagcat aaatgggtca ggacgctttt aatcacatat
      601 aaaaagcgtc ctttttctca caatcaaata atcatcggct tctataacga aagcgatacg
      661 gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggaatggt
      721 tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca
      781 tcagttcctg gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac
      841 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
      901 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
      961 ggcgtcagga cgccgctgaa ttttatttct cacgcttaag ccacctgccg tgggcgatgc
     1021 ttttacactc cggctatgcc gatcatccat atagccgctt tgatattgtg gtcgccgagc
     1081 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca
     1141 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
     1201 gcccagcgca taacgaagat ttgccatttc agggcggcgc gctggggttg tttggctacg
     1261 atctgggccg ccgttttgag tcactgccag aaattgcgca gcaagatatc gttctgccgg
     1321 atatggcagt gggtatctac gactgggcgc tggttgtcga ccaccagcgt caaacagttt
     1381 ctttgctgag tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct
     1441 cgccgcagga agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt
     1501 acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga
     1561 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
     1621 ttaatcaggc caaccgcgcg ccgtttagcg cttttttacg ccttgagcag ggcgcgattt
     1681 taagcctttc accagagcgg tttatccttt gtgataatag tgaaatccag acccgcccga
     1741 ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagagaaac
     1801 tggcgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
     1861 atatcggtcg tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac
     1921 ccttccctgc cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac
     1981 acgccagcga tctgctgcgc gcggcttttc ctggtggctc aataaccggg gctccgaaag
     2041 tacgggctat ggaaattatc gacgaactgg aaccgcagcg acgcaatgcc tggtgcggca
     2101 gcattggcta tttgagcttt tgcggcaaca tggacaccag cattactatc cgcacgctga
     2161 ctaccattaa cggacaaatt tactgctctg cgggcggtgg aattgttgcc gatagccagg
     2221 aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc caactggaga
     2281 agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt
     2341 tgcgcccgca aattaaccgg gaaaccttaa atcatcgtca ggctgccgtg ttaatcccca
     2401 tcgtccgccg accgcaaccg gggttgttgc tgactcagcg ttcgattcat ctgcgtaaac
     2461 acgctggaca agtggcattc cctggaggcg cagtcgatga cacggacgca tcagttatcg
     2521 ccgctgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaggtta
     2581 tcggcgtgct gccgcccgtc gatagcgtca ctggctatca ggtaacccca gtggtcggca
     2641 ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctccgcc gtctttgaaa
     2701 tgccgctcgc ccaggcatta catctgggtc gttatcatcc tttagatatc taccgccgtg
     2761 gcgattcaca tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg
     2821 caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt
     2881 tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg
     2941 attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc
     3001 tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc
     3061 tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga
     3121 aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc
     3181 gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata
     3241 tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc
     3301 ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg
     3361 aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg
     3421 aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt
     3481 attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat gccgccaacg
     3541 aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa
     3601 ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag
     3661 agatcgacga gtatttcgct catgtctggc aaaccatgca ggcatgtatc gatcgcggga
     3721 tgaacaccga aggcgtactg ccaggcccgc tgcgcgtgcc gcgtcgtgcg tctgccctgc
     3781 gccggatgct ggtttccagc gataaactgt ctaacgatcc aatgaatgtc attgactggg
     3841 taaacatgtt tgcgctggca gttaacgaag agaacgccgc cggtggtcgc gtggtaactg
     3901 cgccaaccaa cggtgcttgt ggcattgttc cggcagtgct ggcttactat gaccacttta
     3961 ttgagtccgt cagcccggac atctataccc gctactttat ggcagcgggt gcgattggtg
     4021 cactgtataa aatgaacgcc tctatttccg gtgcggaagt cggttgccag ggcgaagtgg
     4081 gtgttgcctg ttcaatggct gctgcgggcc ttgccgagct gttgggcggt agcccggaac
     4141 aggtttgtgt ggcggcggaa attggcatgg agcacaacct cggtctgacc tgcgacccgg
     4201 ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc tattgcctct gtgaaagcga
     4261 tcaatgctgc acgcatggcc ttacgccgca ccagtgcacc gcgcgtctcg ctggataagg
     4321 tcatcgaaac gatgtacgaa accggtaagg acatgaacgc taaataccgc gaaacctcac
     4381 gcggtggtct ggcaatcaaa gtccagtgtg actaatactt cttactcgct catctgcaac
     4441 ggatgggcga atttatcccc gctttctcgt ctgctgtaat tttccccact acacttccac
     4501 tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa
     4561 cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt
     4621 acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg
     4681 gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag
     4741 gcaggtagcg aagtcttact tccgctaatt ggtctgccct gctctgtcgc ccatttgcca
     4801 ttacgtaaac aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc
     4861 acactttatt gctccagcat ttttggctat cgcaatgtgc ccgtcgtgga cattctggct
     4921 gaacttcctg caccgcaacc acttttacga ctgacgaccg accgtgccct gataaaaggc
     4981 agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag
     5041 atgattaaca tcgacttact gacggcaatg ctccttgaac cacagctgcc gcaaatcagt
     5101 agcgccagcc tgacggtgga cagtcggcat ttgctctatg ggaatgggct ggtagattcc
     5161 cttccgcaac ctgaaaacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc
     5221 attaacgtta atggcccggg ggctacggcg ctggcatggc actatcttcc aacacaatta
     5281 ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac
     5341 cgaatgagct tttcccgcga gatcaatctg ggcctggcgc aacatgagtt cgaattgttc
     5401 tgtcagcctt tgcttaatgc acgcagccag caatgtattg gtgtagagat tttgctgcgc
     5461 tggaacaatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa
     5521 catcatttaa ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat
     5581 gttttcccaa tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc
     5641 cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa
     5701 ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattaccg gattgcccgc
     5761 gagctgcatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca
     5821 ttttcctggc ttgaaacatt acgtcctgac gttctgaaaa ttgataagtc atttaccgca
     5881 gctataggtt ctgacgcggt taactcgacg gtgaccgata tcatcatcgc actggggcaa
     5941 agactgaata ttgaactggt ggcggagggc gtagaaacgc aagaacaggc gaagtatttg
     6001 cgcagtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt
     6061 gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc
     6121 acacccatta tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt
     6181 aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg
     6241 ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc
     6301 cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa
     6361 ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg
     6421 cgtttcgtca gcatccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac
     6481 caagccttgt accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg
     6541 cagcacgccc agcaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc
     6601 cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg
     6661 tacgatacca atgatttcat ccagttcacc gcgacatacc gggaacagac tgtgcggtga
     6721 agagagcagt tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc
     6781 accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat
     6841 catgtaacgt tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc
     6901 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacggagaa tcgcatcggc
     6961 agtacgcgct cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg
     7021 gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
     7081 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
     7141 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggttttg atgccagcag
     7201 cataaccgcc atcgcaatca ctaccgccgc catcatcact ggcagatggt taaccatccc
     7261 tactgccgta attaccgcat ccaacgagaa gacggcgtca aggatgacga tctgtgtgac
     7321 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
     7381 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat
     7441 caggtcgcgt ccggagaagg aaaaatccat aacggtaaat agcggtttgg tcagcgtgac
     7501 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataatcccag
     7561 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg caatgaagac
     7621 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
     7681 tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
     7741 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa
     7801 aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
     7861 actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt
     7921 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
     7981 ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
     8041 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
     8101 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
     8161 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
     8221 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
     8281 ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc
     8341 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc
     8401 cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc
     8461 gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt ggcgctggca
     8521 gtagaaacag gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg
     8581 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatgggacca
     8641 aacgactaca tggttattgg ccttgctcgt atcgacgacc gtctgattca cggtcaggtc
     8701 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
     8761 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
     8821 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
     8881 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
     8941 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
     9001 aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt
     9061 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
     9121 agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt
     9181 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
     9241 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
     9301 tcgcgtgtac cctggtgggt atcgttcttg gcgatatgaa aaccggtatt attatcggtg
     9361 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg
     9421 ccgctctggc ttccatcatt tctactattc tggttatcgc aggtcatcag agcattggtg
     9481 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc
     9541 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
     9601 cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
     9661 cggccgtcat cgttgcgttg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
     9721 ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt
     9781 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
     9841 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
     9901 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
     9961 aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    10021 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc
    10081 tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    10141 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    10201 aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc
    10261 tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    10321 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    10381 tctggggaac ggtacgtccg gtatttgcag cgctgggggc tggtatcgcg atgagcggca
    10441 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    10501 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    10561 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    10621 tggttaataa gtggacacat gtcaacatcc cgctggtggt ctctcgcatt actgaccaga
    10681 cgggcaaaga acacgttacc actgtccaga ctattctgga ccagttaatg ccaggccttg
    10741 tgccactgct gctgaccttt gcttgtatgt ggctgttgcg caaaaaagtt aacccgctgt
    10801 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    10861 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    10921 tgacaatcac ggacctggta ctgattcttt tcatcgccgc acttctggcc ttcgcgatct
    10981 acgatcagtt catcatgcac cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    11041 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    11101 acgtcacgaa tcatggcgcg cttataacaa catggttatt aagtgcactg gctctgatgg
    11161 gtttttatat attctggatt cgcgttccga agatcatctt taaacaaaaa ggttttttct
    11221 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    11281 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatt
    11341 tggaaaaaat ttataagctt ctcgtttcaa ctcaataagt tatgaattta gccaaagcta
    11401 tgt
//
LOCUS       DAZFRE010000134        11312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFRE010000134 DAZFRE010000000
VERSION     DAZFRE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.134"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..911
                     /locus_tag="ACHKUI_004421"
     CDS             <1..911
                     /locus_tag="ACHKUI_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6600488.1"
                     /translation="KVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGF
                     IGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSAVLVDLNDQGERSFTFMVRPSA
                     DLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDL
                     WQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDREICALAKEYEIAMLLVTKG
                     AEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRQIIDL
                     AQRCGALAVTAKGAMTALPCRQELEW"
     gene            980..2227
                     /locus_tag="ACHKUI_004422"
     CDS             980..2227
                     /locus_tag="ACHKUI_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6600489.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDQEHQCVAADVGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2307..2459)
                     /locus_tag="ACHKUI_004423"
     CDS             complement(2307..2459)
                     /locus_tag="ACHKUI_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600490.1"
                     /translation="MLSLMNSQARDTRDRSLNYDNALEISEPCIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(2667..2741)
                     /locus_tag="ACHKUI_004424"
     tRNA            complement(2667..2741)
                     /locus_tag="ACHKUI_004424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2706..2708),aa:Arg,seq:cct)
     gene            complement(2817..3749)
                     /gene="yfdC"
                     /locus_tag="ACHKUI_004425"
     CDS             complement(2817..3749)
                     /gene="yfdC"
                     /locus_tag="ACHKUI_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6600491.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSVLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNIIGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKVEARQKAERAENIKKNDKNPA"
     gene            4043..4798
                     /gene="mlaA"
                     /locus_tag="ACHKUI_004426"
     CDS             4043..4798
                     /gene="mlaA"
                     /locus_tag="ACHKUI_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6600492.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4860..6200)
                     /gene="fadL"
                     /locus_tag="ACHKUI_004427"
     CDS             complement(4860..6200)
                     /gene="fadL"
                     /locus_tag="ACHKUI_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6600493.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVAPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6572..6856
                     /gene="yfcZ"
                     /locus_tag="ACHKUI_004428"
     CDS             6572..6856
                     /gene="yfcZ"
                     /locus_tag="ACHKUI_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6600494.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            7050..8360
                     /gene="fadI"
                     /locus_tag="ACHKUI_004429"
     CDS             7050..8360
                     /gene="fadI"
                     /locus_tag="ACHKUI_004429"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6600495.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            8360..10504
                     /gene="fadJ"
                     /locus_tag="ACHKUI_004430"
     CDS             8360..10504
                     /gene="fadJ"
                     /locus_tag="ACHKUI_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6600496.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKVEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADIKMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPTTERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCNRLVEMSERGESFWKTTATDLQ"
     gene            10707..11192
                     /gene="sixA"
                     /locus_tag="ACHKUI_004431"
     CDS             10707..11192
                     /gene="sixA"
                     /locus_tag="ACHKUI_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6600497.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2710 a         2633 c         2966 g         3003 t
ORIGIN      
        1 ccaaagtatg ggttttaggg gatgcggtcg tagatctctt gccagaatca gacgggcggc
       61 tactgccttg tcctggcggt gcgccagcta acgttgcggt gggaatcgcc agattaggcg
      121 gaataagtgg gtttataggt cgggtggggg atgatccttt tggtgcgtta atgcaaagaa
      181 cgctgctaac tgagggagtc gatatcacgt atctgaagca agatgaatgg caccggacat
      241 ccgcggtgct tgtcgatctg aacgatcaag gggaacgttc atttacgttt atggtccgcc
      301 ccagtgccga tcttttttta gagacgacag acttgccctg ctggcgacat ggcgaatggt
      361 tacatctctg ttcaattgcg ttgtctgctg agccttcgcg taccagcgca tttacagcga
      421 tgacggcgat ccggcatgcc ggaggttttg tcagcttcga tcccaatatt cgtgaagatc
      481 tatggcaaga cgagcatttg ctccgcttgt gtttgcggca ggcgctacaa ctggcggatg
      541 tcgtcaagct ctcggaagaa gaatggcgac ttatcagtgg aaaaacacag aacgatcggg
      601 agatatgcgc gctggcaaaa gaatatgaga tcgccatgct gttggtgact aaaggtgcag
      661 aaggggtggt ggtctgttat cgaggacaag ttcaccattt tgctggaatg tctgtgaatt
      721 gtgtcgatag cacgggggcg ggagatgcgt tcgttgccgg gttactcaca ggtctgtcct
      781 ctacgggatt atctacagat gaaagagaaa tgcgacaaat tatcgatctc gctcaacgtt
      841 gcggagcgct tgcagtaacg gcgaaagggg cgatgacagc gctgccatgt cgacaagaac
      901 tggaatggtg agaagtaaac ggcgaagtcg ctcttatctc taaataggac gtgaattttt
      961 taacgacagg caggtaatta tggcactgaa tattccattc agaaatgcgt actatcgttt
     1021 tgcatccagt tactcatttc tcttttttat ttcctggtcg ctgtggtggt cattatacgc
     1081 tatttggctg aaaggacatc tagggttgac agggacggaa ttaggtacac tttattcggt
     1141 caaccagttt accagcattc tatttatgat gttctacggc atcgttcagg ataaactcgg
     1201 tctgaagaaa ccgctcatct ggtgtatgag tttcatcttg gtcttgaccg gaccgtttat
     1261 gatttacgtt tatgaaccgt tactgcaaag caatttttct gtaggtttaa ttctgggtgc
     1321 gctctttttt ggtctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa
     1381 aatggcgcga aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta
     1441 tgctattggc gcattctttg ctggcatatt ttttagtatc agtccccata tcaacttctg
     1501 gttggtctct ctatttggcg ctgtatttat gatgatcaac atgcgtttta aagatcagga
     1561 acaccagtgc gtagcggcgg atgtgggagg ggtaaaaaaa gaggatttta tcgcagtttt
     1621 caaggatcga aacttctggg ttttcgtcat atttattgtg gggacgtggt ctttctataa
     1681 catttttgat caacaactct ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt
     1741 aggaacgcgc ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctgtgcat
     1801 ggcgattatt cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt
     1861 tgtgattatg gcgttgcgta tcctttcctg cgcgctattc gttaacccct ggattatttc
     1921 attagtgaag ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata
     1981 cagcgtggca aattttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat
     2041 tgccagttcg ttggggattg tgctgctttc aacgccgact gggatactct ttgaccacgc
     2101 aggctaccag acggtttttt tcgcaatttc gggtattgtc tgcctgatgt tgctatttgg
     2161 cattttcttc ctgagtaaaa aacgtgagca aatagtgatg gaaacgccag taccttcagc
     2221 aatataaacg taaacttttt ccggttgttg tcgatagctc tatatccctc aaccggaaaa
     2281 taataatacc aaaatgctta gccctgctaa taatcgccta atccaaacgc ctcattcatg
     2341 ttctggtaca gtcgctcaaa tgtactccgg atacacggct cgctgatttc cagggcattg
     2401 tcgtaattca gcgacctgtc ccgtgtatca cgggcctgcg aattcatcaa ggaaagcatt
     2461 gtggagtgaa gtatcaagtc acgccatatt tcgcgatcag gattgtgtgt gatggttaca
     2521 tcgcccagcc tagggctgtt tagtcatcag cgttttctga ccgtgttgag atttcaacct
     2581 gttgcaggaa aaatgagtag atagggcaaa tgtgctgcca aacttatctt ttacggggtg
     2641 aaggtagata tcgtttgaag ggtatctggt gtcccctgca ggaatcgaac ctgcaattag
     2701 cccttaggag gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc
     2761 cgctaatgga ctacggggta agtacacagc cgctcgattg cttaaaccct cgccatttat
     2821 gccgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg acgtgcttct
     2881 actttgcgct tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata
     2941 aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg
     3001 ctccagtgca gcgtaccgtt aaacaccaga tagaggattt ctacagaacc gacaacgatg
     3061 tgggtggtgt cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc
     3121 gcacccgctg caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg
     3181 ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca
     3241 tcgcgagttt cttcattgaa gataggcata tattcaaatg cccacgcagc aatacctgtc
     3301 ccgataatat tacccagcag cacgatgccc cataaccgca taagtaagcc gacgttgctc
     3361 attgtcggtt tttgcatgac gggtagtacc gcagttacgg tgttttcggt aaataattgc
     3421 tggcgggcca ttatgacgat aataaaacca aaggtataac cgagattctc cagcaagacg
     3481 ctgcccggca cgccttccag ttcgacatga aatatccctt ttgccagtag cgaagcgccc
     3541 atcgacaggc ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct
     3601 ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct
     3661 tcatctattt ctattttttt accgcgctct ttttcttcgc tctcaacttc aatttcgtcg
     3721 ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct
     3781 aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg
     3841 ccgcgtttgc ctttttctgt ttctattgaa tcaagtagcc tacagggcgg cgattaccag
     3901 gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg
     3961 cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc
     4021 ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac
     4081 gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga
     4141 agggtttaac cgcactatgt acaacttcaa cttcaatgta ttagacccgt atattgttcg
     4201 accggtcgct gtcgcctggc gtgattatgt tccgcaacct gcgcgtaacg gtttgagcaa
     4261 ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc
     4321 ttatcagggg atggtccact ttacccgctt tttcctgaat accattttgg gaatgggcgg
     4381 ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt
     4441 cggtagtacg cttggtcatt atggtgtggg ttatgggccg tacgttcagt taccgttcta
     4501 cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatagtcttt acccggttct
     4561 ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga tagaaactcg
     4621 tgcgcagttg ttggattccg acggtctgct gcgtcagtcg tccgatcctt atattatggt
     4681 gcgtgaagcg tacttccagc gtcatgattt catcgctaat ggcggtgaac tcaaaccgca
     4741 ggaaaatccg aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga
     4801 aacaaacaaa aaaggtgagt cgcaaaactc accttttttc ttattaagct aacgcgttat
     4861 cagaacgcgt agttaaagtt agtaccgaac agccaggctt taccttcaga ctcgaactga
     4921 tatgggcctt cgttaatttt cacgctctga ccgtgcatat aagaaacacc aacgtcgact
     4981 gaagcatctt tattaaatgc gtaagtcgta cctgcactca gccagaaacg gtcctggtcc
     5041 ggaatggaga tagaacgatt ctgtgccgga actgggctgt catcaaaggc gataccggta
     5101 cggaaggtcc agttatcatc gtagtaataa gtggtaccca acgcgatgcg gtaagcatct
     5161 ttaaagcctt catgtttctg gaacagcgtg tcgccactcg ttgaggtcgc tttcagctgc
     5221 tggaactgac tccagctggt gtaagccagg ctatagtgaa ttgcccactg cggagcaaca
     5281 cggttataac ccgacacttc ccacatttca ggcaggttca gcgtcagata acctgactgc
     5341 gttgcgccac ctgtcgcggt aggaattggc aaaccgtagt tattaaatgc acgattaaga
     5401 tcgctgctgt agttaccttt gaagtcaatt ttcacttcag aacggtaggt cagtgcatag
     5461 cggttatttt tatccagttc atacaggatt ccggcgttcc agccaaaacc ccactggtta
     5521 ccgttcagat gagcgatttt ggtattactg tcgataccgt tggcggtagc tgccaatgct
     5581 tgcccttgct gagtttgtcc agcaggagat tgcataatct gaccggcaac cagctgcccc
     5641 agatcgcctg cgaaacgttc aattttcgcg cgagcgtaga cggcgttgaa accaagacca
     5701 aagctccatg cattatttaa gcgatacgca ccgcttaagt tcaggttcat ggtttcaagg
     5761 tcggttgtac caccgacaga accgcctgca taagtatcgt taaactctgt tgccagacca
     5821 tagttagagg taatagaagc gccccaacca aattggtcgt taatcggtgc aacaaagtgc
     5881 atgttcggaa cccatgccgt aggcgcgatg ttatcggctt tcaggctacg accagacgga
     5941 gacgttccgc tgatatttac atccggatca atataaaccg cacccgcaga aaatgtcggg
     6001 cggtcaaaca tggtaatcaa tgcggggtta cggctaacgt tacctgcatc atcggcaatt
     6061 gcaccttccc ctgaataagc ccggcccagg ccagaggaag aaaattcgtt taactgaaag
     6121 cctgccgacc aggcctgggt ggagataagt gccactgcga ctgcgagagc agactttgta
     6181 aacagggttt tctggctcat gaccataacc tcaatgattt atttttatac aaactatgtt
     6241 acgtgctgta acaggagcgc gaagtgtagg gtctgaggta catcttagaa atcagaccag
     6301 tggcgagagt ataggtcgga ccagctggaa tgttgcaagt atgtttctat cttttttcag
     6361 atatgatcta agaaacgcga tccagctggc aaaattggga ggtggcgcac tcatggattc
     6421 gggtgaattt tgttttagat catttttaag tgtgatttcg gtcacttatc cgattttgca
     6481 gaattaacaa ctggagcagc actttccgga agcgtaaaat atccacaacg tttatctggc
     6541 accttgggtg cacatacaga ggaaatcaac gatgagtaaa tgtagtgctg atgaaacccc
     6601 ggtttgctgc tgtatggatg ttggcaccat tatggacaac tccgactgca ccgcgtctta
     6661 cagccgtgtg tttgcaaacc gcgcagaagc agaacaaacg ctggcggcgc tgactgaaaa
     6721 agcccgtagc gtggaatccg aaccgtgcaa aatcaccccg actttcactg aagagtctga
     6781 cggtgtgcgc ctggatatcg atttcacttt tgcctgcgaa gctgaaatgc tgatcttcca
     6841 gctgggtctg cgttaattcc tctcgggtac atacgcccct gtcagttgct ggcaggggcg
     6901 ttttcttttc tcttcttctt ttctcttctt cttttctctc tgtgtcataa ctcccacttt
     6961 cccttttctc cacttggcta aatgtaaaaa aatggttaag actatgatca ggtcagacta
     7021 ctttatttat ttttttacag gggagtgtta tgggtcaggt tttaccgctg gttacccgcc
     7081 agggcgatcg tatcgccatt gttagcggtt tacgtacgcc ttttgcccga caggcgacgg
     7141 cttttcatgg cattcccgcg gttgatttag ggaagatggt ggtaggcgaa ctgctggcac
     7201 gcaccgagat ccccgctgaa gtgattgaac aactggtctt cggtcaggtt gtgcaaatgc
     7261 cggaagcgcc caacattgcc cgtgaaattg ttcttggcac tggaatgaat gtgcataccg
     7321 atgcttacag cgttagccgc gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag
     7381 aaagcctgat ggcgggaact attcgagcgg ggattgccgg tggggcagat tcctcttccg
     7441 tattgccaat tggcgtcagt aaaaaactgg cgcgcgtact ggttgatgtc aacaaagccc
     7501 gcacaatgag ccagcgcctg aaactttttt ctcgcttacg tttgcgtgac ttaatgccgg
     7561 tgcctccggc agtggcggaa tactccactg gattacggat gggggacacc gcagagcaaa
     7621 tggcgaaaac ctacggcatc acccgagaac agcaagatgc actggcgcac cgttcgcatc
     7681 agcgtgccgc tcaggcgtgg tcagaaggta aactcaaaga agaggtgatg actgccttta
     7741 tcccccctta taaacaaccg cttgtcgaag acaacaatat tcgcggtaat tcctctcttg
     7801 ctgattacgc aaagctgcgt ccggcgtttg atcgtaaaca cgggacggta acggcggcga
     7861 acagtacgcc gctgaccgat ggcgcggcag cggtgatcct gatgactgaa tcccgggcga
     7921 aagaattagg gctggtaccg ctggggtatc tgcgcagcta cgcatttact gcgatagatg
     7981 tctggcagga catgttgctc ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg
     8041 gattgacgat gagcgatctg acattgatcg atatgcacga agcctttgcc gcccagacgc
     8101 tggccaatat tcagttgctt ggcagcgaac gttttgctcg cgacgtactg gggcgtgcac
     8161 atgccactgg cgaagtggac gatagcaaat ttaacgtgct tggcggttcg attgcttatg
     8221 gacatccctt cgcagcgacc ggcgcgcgga tgattaccca gacattgcat gaacttcgcc
     8281 gtcgtggcgg tggatttggt ttagtgaccg cctgtgctgc gggtgggctt ggcgcagcaa
     8341 tggttctgga ggcggaataa tggaaatggc atcagcgttt acccttaatg ttcgtctgga
     8401 caacattgct atcatcacca tcgacgtacc gggtgagaaa atgaataccc tgaaggtgga
     8461 atttgcctcg caggtacgcg ccattattaa gcaactccgt gaaaacaaag agttgcgtgg
     8521 cgtggtgttt gtctccgcga aaccagacaa cttcattgct ggcgcagaca tcaagatgat
     8581 cggcaactgc aaaacggcgc aagaagcgga agtactggcg cggcaggggc aacagttaat
     8641 ggcggagatt catgctttgc ccattccggt tatcgcggct attcatggcg cttgcctggg
     8701 cggcgggctg gagttggcgc tggcgtgcca cggtcgtgtt tgtactgacg atcctaaaac
     8761 ggtgctcggt ttgcctgaag tacaacttgg attgttaccc ggttcaggcg gtacccagcg
     8821 tttaccgcgc ctgataggtg tcagcacagc attagagatg atcctcaccg gaaaacaact
     8881 tcgggcgaaa caggcggtaa agctggggtt ggtggatgac gtcgttccgc actccatatt
     8941 gctggaagcc gctgttgagc tggcaaagca ggatcgccca tcttcacgcc ctctacctgt
     9001 acgcgagcgt attctggcgg ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggtaa
     9061 gaaaacagaa cacaaaacgc aaggtaacta tccgacgaca gaacgcatcc tggaggttgt
     9121 cgaaacggga ttagcgcagg gcaccagcag cggctatgac gctgaagcac gggcgtttgg
     9181 cgaactggcg atgacgccgc aatcgcaggc gctgcgcaat atcttttttg ccagtacgga
     9241 agtgaaaaaa gatcccggca gtgatgcgcc gcctgcgcca ttaaacagcg tggggattct
     9301 aggtggtggc ttgatgggcg gcggtattgc ttatgtcact gcttgtaaag cggggcttcc
     9361 ggtcagaatt aaagatatca acccgcaggg cataaatcat gcgctgaagt acagttggga
     9421 tcagcttgag ggcaaagttc gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct
     9481 ggcattaatc tccggaacga cggactattg cggctttgcc catcgcgatc tgattattga
     9541 agcggtgttt gaaaatctcg aattaaaaca acagatggtg gcggaagtgg agcaaaattg
     9601 cgctactcat accatctttg cttcgaacac gtcatcttta ccgattggcg atatcgccgc
     9661 tcacgccgcg cgacctgagc aagttattgg cctgcatttc ttcagtccgg tggaaaaaat
     9721 gccgctggtg gagattattc ctcatgcgag tacatcggcg caaaccatcg ccaccacggt
     9781 aaaactggcg aaaaaacagg gcaaaacgcc aattgtcgtg cgtgacaaag cggggtttta
     9841 cgtcaatcgc atcttagcgc cttacatcaa tgaagctatc cgcatgttga ccgaaggcga
     9901 gcgtattgag catatcgatg ccgcgctggt gaaatttggt tttccggtag gcccaatcca
     9961 acttttggat gaggtaggaa tcgacaccgg gactaaaatt atgcctgtac tggaagccgc
    10021 ttatggagaa cgttttagcg cgcctgcaaa tgttgtttct tcaattttga acgacgatcg
    10081 caaaggcaga aaaaatggcc ggggtttcta tctttatggt cagaaagggc gtaaaagcaa
    10141 aaaacaggtc gatcccgcca tttacccgct cattggcgca caagggcagg ggcgactctc
    10201 cgcaccgcag gttgctgaac ggtgtgtgat gttgatgctg aatgaagcag tacgttgtct
    10261 ggacgagcag gttatccgta gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg
    10321 ttttccgcca tttctcggtg gaccgttccg ctatatcgat tctctcggcg caggcgaagt
    10381 ggttgcaata atgcaacgac tggccacgca gtatggttcc cgttttaccc cttgcaatcg
    10441 tttagttgag atgagcgagc gtggggaaag tttttggaaa acaactgcaa ctgacctgca
    10501 ataagaaggt caaagttata tgaatccgcg ctgaatggcg gagtgttgct caaaatgtaa
    10561 acgcatattg actatactta cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg
    10621 ataaggcaca atgccggcca ccgttttctt tctctggttt cagatgaaag aaaacgggcg
    10681 aatctggtta acaaaagcgg tgcaatatgc aagtttttat catgcgtcac ggcgacgcag
    10741 ccctcgatgc cgccagtgat tcggttcgtc ctctgactac taacggttgt gacgaatctc
    10801 gcctgatggc gaactggctg aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc
    10861 cgttcctgcg agccgagcaa acactggaag aagtagggga ttgtctgaac ctgccttcca
    10921 gtgcagaagt tctgccggag ttaacgccct gcggcgatgt tggtctggtc ggcgcctatt
    10981 tgcaggcgtt gaccaatgaa ggtgttgctt cggtactggt gatctcccac ttaccgttag
    11041 tcgggtatct ggttgccgag ttatgtccgg gcgaaacgcc gcctatgttt accacttcag
    11101 cgattgccag cgtaacgctt gatgaaagcg gaaaaggcca attcaactgg caaatgagcc
    11161 cttgtaattt gaagatggca aaagcaattt aagcaatcaa tgcccggtta cgtgcgcgta
    11221 caccgggcag tttctccgtc atacaatcat tctttttctt gtctaaatgt tgttaatatt
    11281 taacaactct cgatagttat tatattttcc ca
//
LOCUS       DAZFRE010000135        11210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFRE010000135 DAZFRE010000000
VERSION     DAZFRE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.135"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            402..1016
                     /gene="evgA"
                     /locus_tag="ACHKUI_004432"
     CDS             402..1016
                     /gene="evgA"
                     /locus_tag="ACHKUI_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6600498.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1021..4614
                     /gene="evgS"
                     /locus_tag="ACHKUI_004433"
     CDS             1021..4614
                     /gene="evgS"
                     /locus_tag="ACHKUI_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO6600499.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4670..5815)
                     /gene="yfdE"
                     /locus_tag="ACHKUI_004434"
     CDS             complement(4670..5815)
                     /gene="yfdE"
                     /locus_tag="ACHKUI_004434"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO6600500.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5889..6833)
                     /gene="yfdV"
                     /locus_tag="ACHKUI_004435"
     CDS             complement(5889..6833)
                     /gene="yfdV"
                     /locus_tag="ACHKUI_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6600501.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6903..8597)
                     /gene="oxc"
                     /locus_tag="ACHKUI_004436"
     CDS             complement(6903..8597)
                     /gene="oxc"
                     /locus_tag="ACHKUI_004436"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6600502.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALGAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8651..9901)
                     /gene="frc"
                     /locus_tag="ACHKUI_004437"
     CDS             complement(8651..9901)
                     /gene="frc"
                     /locus_tag="ACHKUI_004437"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6600503.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10413..11045)
                     /locus_tag="ACHKUI_004438"
     CDS             complement(10413..11045)
                     /locus_tag="ACHKUI_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6600504.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3292 a         2519 c         2291 g         3108 t
ORIGIN      
        1 tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcctcat atcaaacaca
       61 gtgcatgttt tgcacattat cccgggaacg ttggagggct aatttaaatg aagtgcaaaa
      121 ataacaacag aggactaaac cgcggctttt gcaatacaat tcttacgcct gtaggattag
      181 tgagaaaact tatagtgctc atttgaaact ataaatcatc ggtatcatcc ctgattttat
      241 tgttgacatt ttatttatgc cgactattta tatggtatac ttgtcgaatt atcttaaagg
      301 aagctcaaat tttcttattt ttattgagaa aatgagatga taccttatgt ctgtattact
      361 acaaggagaa gggaaatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
      421 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
      481 tcttagcaga gttgactgaa ggcggaagcg ccgttcagcg ggtggaaaca cttaagcctg
      541 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
      601 tgagaaagcg ccaatatagc ggaattatta ttattgtctc cgctaaaaat gaccattttt
      661 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
      721 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
      781 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
      841 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa
      901 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcttgatg gaaaaattag
      961 aatgcaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac
     1021 atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagcttc
     1081 gcagacggag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
     1141 ccactacgtc tgagcaacaa ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt
     1201 gcagtacaca agtcccaaac agctacgttg ttgcataccg attcgcagca acggattcgt
     1261 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
     1321 cgggaatacg cagatcatca aaaagcaatg gacgcgctgg aagacggtga agttgatata
     1381 gtgttatcac atttagttgc ttcgccgcct cttaatgatg acattgctgc aactaatcct
     1441 ctgataatta cctttccggc gctggtcact acccttcacg attcaatgcg accgcttacc
     1501 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc ccccagacga ggtaattcat
     1561 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     1621 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     1681 tcccgttatt ttactcactc cttaaatgta gtgaagtatt ataactcacc gcgtcaatat
     1741 aactttttat taaccagaaa agattcaatc gttcttaatg aagtactcaa tcgatttgtt
     1801 gatgctttaa caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac
     1861 ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcag
     1921 catcccgatt taaaggtgct ggaaaatcct tactcgccac cctattctat gacagatgaa
     1981 actggctcag ttcggggcgt gatgggtgat attcttaata ttattacctt gcaaacaggt
     2041 ttaaattttt ctccgatcac cgtttcacat aatattcatg ctgggacaca gcttaacccc
     2101 ggtggatggg atatattgcc cgccgctatt tatagtgaag atcgagaaaa taatgtttca
     2161 tttgctgaag tcttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt
     2221 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gcttcattcg
     2281 caattaaaag agatgtatcc ggaggtagag tggataaaag tcgataacgc cagcgctgca
     2341 tttcacaagg tcaaggaagg tgaacttgat gctctggtcg cgacacagtt aaattcgcgt
     2401 tacatgatcg accattacta tcctaatgaa ctttatcatt ttcttattcc cggcgttcag
     2461 aatgcatcac tttcgtttgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
     2521 aaagcactga atgcaattcc cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa
     2581 atgcccaatg tgaccattga cacatgggac ctctatagcg agcaatttta tattgttacg
     2641 acattatccg ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca
     2701 gttcgtcgtc gtaaagttat tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg
     2761 ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     2821 cacaatagtg catttgaaca ttatttcact gctgattact ataaaaatgc aatgttacca
     2881 ttagaaaata gtgaatcccc ctttaaagat gttttttcta atacgcatga agtcacagca
     2941 gaaacgaaag aaaaccgaac aatatacaca caggtatttg aaattgataa tggcatcgag
     3001 aaaagatgca ttaatcactg gcatacctta tgtaatctgc cagcaagcga acatgctgtt
     3061 tatatttgtg gttggcaaga tattaccgag acgcgtgatt taattcatgc actcgaagta
     3121 gagagaaata aagcgatcaa tgcaactgtc gcaaaaagcc agtttctggc aacaatgagt
     3181 cacgaaataa gaacaccaat aagctccatt atgggcttcc tggaactact gtcgggttct
     3241 ggtcttagca aggaacaacg ggtggaggcg atttcacttg cctacgccac cggacaatca
     3301 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
     3361 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcgct
     3421 gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacgcttcc tgaacgctac
     3481 ctggttaaga tcgaccctca ggcatttaag caggtcttat ctaatttact gagtaatgct
     3541 ctcaaattta ctaccgaggg ggcagtaaaa attacgacct ccctgggtca cattgatgac
     3601 aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa
     3661 caacaacaac tgtttaaacg ctatagccaa acaagtgcag gtcgtcagca aacaggttct
     3721 ggtttaggct taatgatctg caaagaatta attaaaaaca tgcagggcga tttgtcatta
     3781 gaaagtcatc caggcatagg aacgatattt acgatcacaa tcccggtaga aattacccaa
     3841 caagtggcgg ctgtcgaggc aaaagcagaa caacctataa cactacctga aaagttgagc
     3901 atattaatcg cggatgatca tccgaccaat aggctattac tcaaacgcca gctaaatcta
     3961 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
     4021 caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg
     4081 actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca
     4141 caggctaacg aacgtgaaaa agggttaaat tgcggcatga acttatgttt gttcaaaccg
     4201 ttgactctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca
     4261 cctcagtatc gccaccttga tatcgaggcc ctgaagaata atacggcgaa tgatctacaa
     4321 ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg
     4381 tttcatgcac tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac
     4441 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccacca gttagaaata
     4501 acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa
     4561 cacattgcag agctggacca ggagattact gttttctgtc agcaaaatga ctaaatagcg
     4621 gctcccacaa tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
     4681 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
     4741 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa
     4801 tcaacatatt tctcgcctga gtttgtggta aattaatggc ctcagccaca cttaataacg
     4861 gcgcgacggg cacaccaact tcatgtattc tggctaacca aacttcagct acctgcgttt
     4921 ttaacgtccg ctcaatatat tctttaagaa tagcctggtt atgtacgcgt aaaatattgc
     4981 tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa
     5041 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa
     5101 aaggtgccat gtagggatgg cgatttccca ggcgttgtgg tgacttccca gtcgcgatat
     5161 atgccatcag tccatgctcc agaaaactca gagtggcatc aaacatcgct atatcgacat
     5221 gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattcca ctgaataaat
     5281 aaacaccgcc acagagatcc gcaagagagg taccaacgcg cactggcgga gcatcagggt
     5341 atcctgtttc catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat
     5401 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatataagg cgcgggttga
     5461 tttcttgtaa cctttcccat gaaaacccga gtttttccat tgtacctggg cgaaaattct
     5521 cagctaatac atcagcttgt ttaagcatat ttataaatat acttttatcg tgatcattct
     5581 ttaaatcaag aaccacactc tcttttccat gattaataaa actgtaatag agcgactgtc
     5641 catccacata gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa
     5701 ttacccttgc acccatatta caaagaagtt gagttccgaa aggcccatta aggacatgcg
     5761 tcatatcgat aactaataag ccttcaaacg gccctttgca ttcattattt gtcatttgct
     5821 tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac
     5881 atgattaatt atgaaaccag tcgactgaca taaatccaca aaggagccgt gacgacaaaa
     5941 cccagtacgc tcacagccaa tgacgctgta ccagtgcggg tgtagacatt aaaccggctg
     6001 gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaaa
     6061 tgttcgctat tcaaatgaca tgccatacct acgagaagca gcgccagtgg catcagaatc
     6121 agcttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga
     6181 gtcaaccccg cagcgaatac cgccacccct gagttagcct tcgcaatcag attaaaggtt
     6241 gggtcccatg ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt
     6301 gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta
     6361 ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta
     6421 acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga
     6481 tcgagaactg caaaccccag gaatccaatg gtaggtgaac ctgcaattaa tgcacatact
     6541 gctgcttctg catgggtacg tttaaaaaat ttgtagcaac cgaaccagga gaaaaagaaa
     6601 catccgacaa taaccacaag tgatacaagg gtcaggcgag tgtccgcaaa aatcatttcc
     6661 ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt
     6721 ttattaaatg cccgagcttg atcctctgaa aaagtttctc gtctgccgct aaaataaccc
     6781 aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta
     6841 tttccgataa gggtcccctt accatcgcat aataagttgg caagaggaga cgatgtcagt
     6901 atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct
     6961 gctgctgggt cgatgaccac attaataatg gtcggtttgc gcgactgaat tccggtggtt
     7021 aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc
     7081 attaatttgt cataccttgc atggtgcaac agatccgttg gcgatggtgc accagcgccg
     7141 ctgaggtcaa caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc
     7201 ggcaggttat atcgacaaat cgtttcaatc tccatcccac tgaaaccaaa agcactatca
     7261 ccttcaatgg cgacaaccgg agaaccagag gtcacgctag caccgatggc atagcccata
     7321 ccgatgccca tgacacccca ggtgccacaa tccagacgac gacgtggttt atacatatca
     7381 ataatatttc gtgcattatc cagggtattt gccccttcat taactaaata aatatcctgg
     7441 ttctcgcgca aaacatcgcg cacggcaccc aatgcattaa agtaatttaa tggttgggta
     7501 tctgtactta acttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata
     7561 tcgcgccaca ccagtggagt cgtgaatgtg ttttgtttca attctgccag cataccttgc
     7621 atactggatg caatatcgcc aacgactggc acagcaatgg ggcggttgct gtcaatttcc
     7681 tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt
     7741 gccagtaacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca
     7801 aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt cgaggatccc tttcgccata
     7861 gacattggca ggaatggaat ctgggcactt tcaataaatt cacgaagctg ttcatcagat
     7921 tgtgaatacg ccgcgccttt gccaagaata attaatggtc gttcagcttt tgctaaaagc
     7981 gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact
     8041 ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc
     8101 aaatcaagat aaacaccgcc aggacgaccc gatactgaga cccggatagc gcgtgccaat
     8161 gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc
     8221 gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg
     8281 ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcattggc caatgcggtc
     8341 aaaccgttga ggaatcctgg cgcagacact gtcaggcaga tccctggttt ttgggtaaga
     8401 aaaccgcttg ctgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga
     8461 atgccttccg cctgggcatg gcgcgccata tccgtcacag gaatacctac aacaccataa
     8521 atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt
     8581 tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc
     8641 cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac
     8701 ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg
     8761 gcgtaaaggc agagaatttc attggacaac caactgtcag atattttcca cgcaacggtt
     8821 gttccacttc gacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca
     8881 tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt
     8941 tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc
     9001 gggcatgggc tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac
     9061 aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt
     9121 cccagccttt acatttcagg atccagccag gctgaccgcc accacccgca ttaccaccgc
     9181 ggggaactgc atcaccaaat gtgccattcg gatactgcgg gtattcttcc agatacccca
     9241 acttatcgag acgctgctgg tcgcgtaatt tcacgcggca aaggttcaac acggcatcct
     9301 gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa
     9361 gtaaaccgat cagcaaatgc attcctgtgt tgctgtcacc caacgctgca gcgcttacca
     9421 gcggcggacc gtcccaaaaa cctgtagtag atgccgcgcc acccgctgcc tgagcaacgt
     9481 tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa
     9541 aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccata tgatcaatgg
     9601 cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct
     9661 ctttgccttc cgctgttttg gtatttaatt caatagaacg tttgttactg ttaagcatgg
     9721 tgaagtaaag cgcatcgata tcaggaatat ctcgcagctg gtggcgcgtt acgtcaccaa
     9781 cgccgggacg ttcaatttta atgacgtcag cgccaaacca ggccagcatt tgagtacaag
     9841 atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca
     9901 tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta aggcataacg
     9961 cctctataaa taatctgcaa aatatcgtcg tagctgcact cgatattttt aaattcctgt
    10021 atcaagtgct gaaaatcatt tcatcgctac ttccaattcc tttctgcatg gtaagtagtg
    10081 aggacgaaaa gaagaatata ataaatattg gttcgcggta atcagagaga gccgcgacat
    10141 ttgtgatgtt attcttacaa ctgtaagaag tgtcagtgat gtacaagtaa gggtatgatt
    10201 acactattta aagcgtacca taaaatataa cataataaat gatttgtgat aatatgggat
    10261 tattgcggtt tactttgagg aagataaaat gcgagtttat agaaccgcga gcgccaaata
    10321 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcgactg gagggagtta
    10381 aatcctttac ctcaatatat tgcctgtcag gtttagttca caaacagtgc ttcgctatca
    10441 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
    10501 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcaaatat
    10561 tggttttcca ttacaccaac accggccagg cgaagctctt ccattgcacc ctttttatcc
    10621 cctttcgcca ttttttcgtt ggcaattttt atcgcggctt ctttttcggg tgtcgctaca
    10681 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgacgttg
    10741 gtctttttac ctggtttagc gaactttgcc cattcggtac tatcatctga cagcaaagcg
    10801 gaagcttcat tggtcagttc ttttgccttt tccggatcgc catgaaacag agccaggcgc
    10861 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc
    10921 gttttctggg tttgcttaac ttgctgagtt tgctgttgtg ccgctaccgg tgcattatca
    10981 gcagcccata cagttgaaga tgccagaatt gctgtgacca tcgtagccat aattaaacgt
    11041 ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat tgggtgagaa
    11101 tgaaatgaga aatcagagag caacggacat tttgaaaggg ttatcttaca actgtagtta
    11161 ttatcgcaaa gccgctttta gtcttacagt tgtaggaatc tgcttcctgt
//
LOCUS       DAZFRE010000136        11173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFRE010000136 DAZFRE010000000
VERSION     DAZFRE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.136"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            2..100
                     /locus_tag="ACHKUI_004439"
     CDS             2..100
                     /locus_tag="ACHKUI_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6600505.1"
                     /translation="MFFTDYSHFMDRITLPSTDTEKGKGKWKIITA"
     gene            79..183
                     /gene="speFL"
                     /locus_tag="ACHKUI_004440"
     CDS             79..183
                     /gene="speFL"
                     /locus_tag="ACHKUI_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6600506.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            493..2691
                     /gene="speF"
                     /locus_tag="ACHKUI_004441"
     CDS             493..2691
                     /gene="speF"
                     /locus_tag="ACHKUI_004441"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6600507.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2688..4007
                     /gene="potE"
                     /locus_tag="ACHKUI_004442"
     CDS             2688..4007
                     /gene="potE"
                     /locus_tag="ACHKUI_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6600508.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4064..5704)
                     /gene="pgm"
                     /locus_tag="ACHKUI_004443"
     CDS             complement(4064..5704)
                     /gene="pgm"
                     /locus_tag="ACHKUI_004443"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6600509.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5730..6275)
                     /gene="seqA"
                     /locus_tag="ACHKUI_004444"
     CDS             complement(5730..6275)
                     /gene="seqA"
                     /locus_tag="ACHKUI_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6600510.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6460..7224
                     /gene="ybfF"
                     /locus_tag="ACHKUI_004445"
     CDS             6460..7224
                     /gene="ybfF"
                     /locus_tag="ACHKUI_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6600511.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7364..7657
                     /gene="ybfE"
                     /locus_tag="ACHKUI_004446"
     CDS             7364..7657
                     /gene="ybfE"
                     /locus_tag="ACHKUI_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6600512.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7797..8327
                     /gene="fldA"
                     /locus_tag="ACHKUI_004447"
     CDS             7797..8327
                     /gene="fldA"
                     /locus_tag="ACHKUI_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6600513.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8537..8623
                     /gene="uof"
                     /locus_tag="ACHKUI_004448"
     CDS             8537..8623
                     /gene="uof"
                     /locus_tag="ACHKUI_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6600514.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8616..9062
                     /gene="fur"
                     /locus_tag="ACHKUI_004449"
     CDS             8616..9062
                     /gene="fur"
                     /locus_tag="ACHKUI_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6600515.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9146..9472)
                     /gene="chiQ"
                     /locus_tag="ACHKUI_004450"
     CDS             complement(9146..9472)
                     /gene="chiQ"
                     /locus_tag="ACHKUI_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6600516.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9522..10928)
                     /gene="chiP"
                     /locus_tag="ACHKUI_004451"
     CDS             complement(9522..10928)
                     /gene="chiP"
                     /locus_tag="ACHKUI_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO6600517.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAIFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2732 a         2786 c         2765 g         2890 t
ORIGIN      
        1 gatcttcttc acagattata gccatttcat ggatagaata accctacctt caactgacac
       61 agaaaaaggt aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat aaggcggaca
      121 acccatatca tgaagtttgc ccatcgtaat agttttgact ttcatttctt taatgcccgc
      181 tagtcttctg actaaagggc accccagcgt acaggtctcc ctgactttaa gcattacagg
      241 ttaatatctg tatccctcgg tgctcatata ctgctaaccc tcttaaactc taaataattc
      301 gagtcgcagc acatgcaact tgaagtatga cgagtatagc cagttaccgg gctggtctgg
      361 gttattgcat ctgcaaaaag caaactactg atttatttat cagcggtggg gttttgcttt
      421 tttccggcgt gatcaatttc tcctttgaga aattgaggac ctgctattac ctgaaataaa
      481 gagatgaaca aaatgtcaga attaaaaatt gccgttagcc gttcttgccc ggattgtttt
      541 tccactcatc gtgaatgcgt gaatattgac aaaagtaatt atattgacgt tgccgccatt
      601 attttatcag tcaatgatgt tgaacgtgga aaactcgatg aaatagacgc tactggctat
      661 ggcattcctg tttttattgc aacggaaaat gaagaacgtg taccagcaga atatctgcca
      721 cgtatttctg gtgtcttcga gcattgcgaa tcacggaaag agttttatgg acgtcagtta
      781 gaaaccgctg ccagccatta tgaaacgcaa ctgcgcccgc ccttcttccg cgcactggtc
      841 gattatgtca atcagggcaa tagcgctttt gactgcccgg gtcatcaggg aggtgagttt
      901 ttccgtcgcc atccggcagg caatcagttc gtggaatatt ttggcgaaat gctcttccgc
      961 tctgacttgt gtaatgccga cgttgcaatg ggtgatcttc tgattcatga aggcgcgcca
     1021 tgcattgcac agcaacatgc ggcaaaagtg tttaatgccg ataaaacgta ctttgtttta
     1081 aacggcacct cctcctccaa caaagtggtt ttaaacgccc tgctgacacc aggtgatctg
     1141 gtgctgtttg accgcaataa ccataagtct aaccatcacg gggccttgct acaggctggt
     1201 gcaacaccgg tttatctgga aacagcacgt aacccgtatg gttttatcgg tggcattgat
     1261 gcgcactgct tcgaagaaag ttatctgcgt gaactgatta cagaagttgc accgcaacgg
     1321 gcaaaagagg cgcgtccgtt ccgcctcgct gtgattcagt taggcaccta cgacggtact
     1381 atttataacg cccgccaggt ggtggataaa atcggtcatc tgtgtgacta catcctgttt
     1441 gactcagcat gggtcggcta tgaacagttt attccgatga tggcggactg ttcgccactg
     1501 ctgctggagc ttaatgagaa cgatccgggt attctggtta cgcaatctgt gcataaacaa
     1561 caggctggtt tttctcagac ttcacaaatt cataaaaaag acagccatat caaagggcaa
     1621 cagcgttatg taccgcacaa acgcatgaac aacgcattta tgatgcacgc ctccaccagc
     1681 ccgttctatc cgctgtttgc cgcactggat atcaacgcca aaatgcatga aggtgtcagc
     1741 ggtcgtaata tgtggatgga ttgtgtggtt aacggtatta atgcccgcaa attgatcctc
     1801 gataactgcc agcacattcg cccgttcgtt cctgaactgg tggatggtaa accctggcag
     1861 tcgtatgaaa cagcgcaaat tgcggttgat ctgcgcttct ttaaatttgt acccggtgag
     1921 cactggcatt cttttgaagg ctacgcagag aaccaatact ttgtcgatcc gtgcaaactg
     1981 ttgctgacaa ctccaggtat tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc
     2041 gcaacgattc ttgctaactt cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt
     2101 aactccatcc tcttcctgct gactccggca gaagatatgg ccaaactgca gcaacttgtt
     2161 gccctgctgg tacgcttcga aaaactactg gaagctgatg cgcctctggc ggaagtactg
     2221 ccttccatct acaaacagca tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc
     2281 caggaaatgc atgatttgta tgcccgccac aacgtgaaac aactgcaaaa agagatgttc
     2341 cgtaaggaac acttcccacg cgtcagcatg aatccgcaag aagccaacta cgcctactta
     2401 cgcggtgaag tggagctggt tcgtctgccg gatgcagaag gccgtatcgc tgccgaaggt
     2461 gcgcttcctt atcctccggg tgtgctgtgt gttgttcctg gtgaaatctg gggtggtgct
     2521 gttctgcgtt acttcagcgc tctggaagaa ggcatcaacc tgctgccagg ttttgcaccg
     2581 gagctgcagg gtgtctatat cgaagaacat gatggtcgta agcaagtttg gtgctatgtc
     2641 atcaagcctc gtgatgcgca aagcgccctg ttgaaagggg aaaaattatg agtcaggcta
     2701 aatcgaacaa aatgggtgtc gttcagttaa ccatactgac gatggtcaac atgatgggct
     2761 ccggtatcat catgctgccg acaaagcttg ccgaagtcgg gacaatctca attatctcct
     2821 ggctggtgac agccgtaggc tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt
     2881 tcagccgtaa atcaggaggt atgggcggtt atgccgagta tgctttcggt aaatccggta
     2941 actttatggc gaactatacc tacggcgtct cgctgctgat tgctaacgtc gcgattgcta
     3001 tttcggcggt tggttacggc accgaactgc tcggcgcgag tttgtcgcca gtgcagattg
     3061 gtcttgcgac catcggggtg ctgtggattt gtaccgtggc taactttggt ggtgcgcgca
     3121 ttaccgggca aatcagtagc attaccgtgt ggggggtcat tattccggtg gtaggcctgt
     3181 gcattatcgg ctggttctgg tttagcccga cactttacgt tgattcctgg aatccgcatc
     3241 atgcaccgtt cttcagtgcg gtaggttctt ccatcgctat gacgctgtgg gcttttcttg
     3301 gtctggagtc tgcgtgtgcg aatactgatg tagtggaaaa cccggaacgt aatgtgccaa
     3361 tcgcggtact cggcggtacg ttaggtgcgg cggtgattta tatcgtctcc accaacgtga
     3421 ttgccgggat tgtgccaaat atggagctgg caaattcaac ggcaccattt ggtctggcct
     3481 tcgcgcagat gttcacgccg gaagtgggta aagtcattat ggcgctgatg gtgatgtcct
     3541 gctgcggttc gctgcttggc tggcagttca ccattgccca ggtgtttaaa tcttcatctg
     3601 atgaaggcta cttccctaaa attttctccc gtgtaaccaa agtggatgca ccggtgcagg
     3661 gaatgttgac cattgtgatt atccagagcg ggttggcgct gatgacgatt agcccgtcgc
     3721 taaacagtca gttcaacgtg ctggttaacc tggccgtggt gaccaatatc attccgtata
     3781 ttctgtcaat ggcggcatta gtcattattc agaaggtcgc taatgtgccc ccctcaaaag
     3841 cgaaagttgc aaactttgtt gcttttgttg gcgcgatgta cagcttttat gcgctgtact
     3901 catccgggga agaagccatg ctatacggtt ccatcgtgac cttcctcggt tggacactgt
     3961 atggtctggt ctcaccacgc tttgaactga aaaataaaca cggttaatta agcaaagcaa
     4021 aaaagggcga tcttccgacc gccctttttc ttatttgata tgtttacgcg tttttcagaa
     4081 cttcgctaac aatctcaacc gcttctttct caatctgctt gcgatgttct tcaccaagga
     4141 agctttcgca gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc
     4201 cgttgtcagt catcactttc agaccgccga tagaagcgcc attacccggt gccgccgtca
     4261 gacgcgcggt gatcgggtca cctgccaggg tgctggcgct caccatttcc ggagacagct
     4321 tagacaacgc cgctttttgt gcggaagtcg ctgctgcctg caaacggtta tagctcggcg
     4381 caccaaagcg ttttgccagt tcgttatagt gttcctgcgg gttcttaccg gtgacagcgg
     4441 tgatttccgc cgccagcaga cacataatga tgccgtcttt atcggtggac cacggcgtgc
     4501 cgtcgaaacg caggaaggaa gcgcctgcac tctcttcgcc gccaaagccg aagctgccat
     4561 cgaacaaacc atcaacaaac catttgaaac ctaccggtac ttctaccagc ttacggccca
     4621 aatcattgac cacacggtcg atcatcgcag atgaaaccag cgttttaccg acagcaacat
     4681 ctttgcccca ctgcggacga tgctggaaca ggtagttgat cgccaccgcc aggtagtggt
     4741 tcggattcat caaacctgcc ggagtgacga taccgtgacg gtcataatcc gggtcgttag
     4801 caaacgccag atcgaactta tcacgcagtg ccagcaggcc cgccatcgca cactcggagg
     4861 agcagtccat acggatcgca ccgtctttat cgaggtgcat aaagcggaag gtttgatcga
     4921 cctgatcgtt aacgatggtc aggttgaggt tgtaatactc gccaatgcgc ttccagtatt
     4981 cgataccgga accacccagc ggatcaacgc ccagcgtcag gcccgctttc tgaatcgccg
     5041 ccatatcaac gatatcggcc agcccttcca caaacggctg caccagatcc tgctctttca
     5101 catgaccgga tgccatcgct tcgtcgaggg agatacgctt cacgcctttc aggccatcag
     5161 ccagcagtgc gtttgctctg tcttccacca ctttagtgac gttggtatca gccgggccac
     5221 catttggcgg attgtacttg ataccaccat cttccggcgg gttatgggac ggtgtaatca
     5281 cgataccgtc tgccagcggg ccaccttttt tattgtgaac cagaatggca ttggaaatgg
     5341 caggtgtcgg agtgaagcca ttgttttcct gcacaatgac atcaacgccg ttcgctgcca
     5401 gcacttccag tacggaaatg aacgcaggct cggacagggc gtgagtatct ttacccacat
     5461 agcaagggcc agtgatgccg tttttcgcac gttcttcagc aattgcctga gcgatcgcca
     5521 gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga
     5581 atttcaccgc gtgctccgca ttccctgctt ctggtttcag tacataatat tgcgccgtca
     5641 gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga ttgtggattg
     5701 ccattgcttt gtcctttgtc tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat
     5761 tccgccggga attgcatcga ctgcatgatg tgttcgatca tgctgcattt acggccggtg
     5821 ttggtgttgg tgatcaccca atacggcgta ccaggcacat gtttcggctt ggtctgatta
     5881 ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac
     5941 gattccgttg cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga caacagcagc
     6001 ataaagcgat tgaccgctcg cttttgctct gcgtattcgt ccgaaagcag aagttcacgc
     6061 attgcgcgaa ccttgtcttt aatcgttttg accggcttcg cttcgacgat agcaggtgac
     6121 gcaacgcgaa cctctttcgt caccggagca gcaggctgtg atgcggcgga aaatttcaac
     6181 atacgccgta aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct ggcaatatag
     6241 ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct
     6301 gaatacaagt cgccaggagt atatgaataa atcccggcag cggatagcgt caagcctggc
     6361 ttgcagaaaa actcggaata aaccttattt gttgcagccc tgtggcacaa tggtcaacat
     6421 aataccctaa cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg
     6481 caaactgcac aaaaccagca caataattct cccatcgttc ttgtccatgg tctgtttggc
     6541 agcctcgaca accttggcgt actggctcgc gatctggtaa acgatcacaa tatcatccag
     6601 gttgatatgc gtaaccacgg tctttcaccg agagatccgg taatgaatta cccggcgatg
     6661 gcgcaggatc ttgttgatac cctggatgca cagcagatcg acaaagcaac atttatcggt
     6721 cactccatgg gcggtaaagc ggtaatggct cttactgcac tagcccccga tcgcattgac
     6781 aagctggtgg cgattgatat tgcgccggtc gattatcacg ttcgccgtca tgatgagatt
     6841 ttcgcggcca tcaacgcagt cagtgaatca gacgcacaaa cacgccagca agcagcggca
     6901 ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt
     6961 gacggggagt ggcgctttaa cgtgccagta ttgtgggatc agtatccgca tattgtgggt
     7021 tgggagaaaa tcccggcatg ggatcaccct gccctgttta tccctggcgg caattctccg
     7081 tatgttagcg agcagtaccg cgatgattta ctggcacaat ttccacaggc acgggcgcat
     7141 gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt attacgcgct
     7201 atccgtcgct atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt
     7261 ccgcgcgtgg acgttggcgt gactcatcgc gctgatgtat tatggcgcgc tatccatccg
     7321 ggcagaggcc tggctgattg tttcccccga agtcaccaag atcatggcca aagaacaaac
     7381 ggaccgtacg acattagatc tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa
     7441 tccgctttcg cgcgatgaac agctgcgtat taataaacgc aaccagctaa aacgcgacaa
     7501 agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa
     7561 cgagctggcg gaatcgcgca atatgagccg tagcgaactg atcgaagaga tgttaatgca
     7621 gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt tccactttca tgtagcacag
     7681 tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct gttctgctat gattgccttt
     7741 atccgtgggc aattttccac ccccatttca ataagtttca agaggttatt tcactcatgg
     7801 ctatcactgg catctttttc ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga
     7861 ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga cattgcaaaa agcagcaaag
     7921 aagatctgga agcttatgac attctgctgc tgggcatccc aacctggtat tacggcgaag
     7981 cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga gattgatttc aatggcaaac
     8041 tggttgcgct gtttggttgt ggtgaccagg aagattacgc cgaatatttc tgcgacgcat
     8101 tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac catcgttggt cactggccaa
     8161 ctgcgggcta ccatttcgaa gcgtcaaaag gtctggcaga tgacgaccat tttgtcggtc
     8221 tggctatcga cgaagaccgt cagccggaac tgaccgctga acgtgtagaa aaatgggtta
     8281 aacagatttc tgaagagctg catctcgacg aaattctcaa tgcctgatgt gatgcggcgt
     8341 agactcatgt ctacgccgta ttaatagata atgccaatca aaataattgc tacaaatttg
     8401 taacttttgc tgttgtacct gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa
     8461 tgtaagctgt gccacgtttt tattaacaat atttgccagg gacttgtggt tttcatttag
     8521 gcgtggcaat tctataatga tacgcattat ctcaagagca aattctgtca cttcttctaa
     8581 tgaagtgaac cgcttagtaa caggacagat tccgcatgac tgataacaat accgccctaa
     8641 agaaagctgg cctgaaagta acgcttcctc gcttgaaaat cctggaagtt cttcaggagc
     8701 cggacaacca tcacgtcagt gcggaagatt tatacaaacg tctgatcgat atgggtgaag
     8761 aaattggtct ggctacggta tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca
     8821 cccgccacaa ttttgaaggc ggtaaatccg tatttgaact aacacagcaa catcaccacg
     8881 atcacctgat ctgcctcgac tgcggtaagg ttatcgaatt tagtgatgat tccatcgaag
     8941 cgcgtcagcg tgaaatcgcc gcaaaacatg gcattcgcct gactaaccac agtctctatc
     9001 tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat
     9061 aagccagcct gaacgagaaa agccaacctg caggttggct tttttataca agggaaataa
     9121 gaaaagcgag agttacagct ctcacttatt tgttattact gcgaatttcc tgccagacct
     9181 tatcacaatc ggctttcacc gcctgatcat ttccggtttg cgttgcctga atacactgct
     9241 gataatccag tacgcggaca ctttcctgct cggcaaactg ctgatgttgt ttctctttct
     9301 tcagcacgtt taacacgctc tggcaggctt caattttttc cggcgaacct tgcgcggtgt
     9361 tgatacaggc gctgtaagcc tctttcagac gactgtcttc ctgcggtgcg gttgattgcg
     9421 cacaagctac cagccccgat gccatcatgg cgatgagaat cagttttttc atagcaaatt
     9481 cctcaaacgt gcggcaggca caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt
     9541 gcgattacca tgaatttcac gtcgcgctca tcctggaaaa tgttgccgta accaccgccc
     9601 cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt gcagtttgaa catcgtgccc
     9661 ttggcacgac cgtcctgaat ggtatacacc gcatccaggc tgtaggcaga ctcttcaata
     9721 gtccggtttt tatcgtagta cgcatccgga ttgctctgcc aggtcgcagg tttagcatcc
     9781 catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa gattccagtt tttcaggtca
     9841 tacatcgcac cgaagaagat cgctttttcg ccgttggcgt tgaagtcaga acggttatcc
     9901 caccagatat ccaggcgacc gttcgaggaa gcgtaggttg gagtcatacg ttgcaggaag
     9961 tatccttgct gaccgtcagc cttaacccag gtaccttcga ggcgcaaatc cactacgtca
    10021 gccgcccggt aaccaaaggt caacgcctgc agccaggcgg tgccgtcata aagatcgttg
    10081 acgctgcgat cgtcaacttt atcgcgcgta ccatagaact ggtagctggt ggttaacggg
    10141 ctaccggcga tatcaaattt gtagctggct ttggcaaaat attgatcgat atacccttcg
    10201 gcctgaccaa acgccgcttc cagtacgaag ttatttttga agtcgtactt cgccccaagg
    10261 gagtgtagat aatcaacttt ggtggtttta tcgttctgat aaaactcatc catttccaga
    10321 tgccacggcg ctttgtattc gttggtccac atgtaggaga aactcaacgc accagcatcg
    10381 ccgtaatcaa aattcgcccc ggcctccgcc ccctgataag tacccggcat aaagctccag
    10441 tgtggcgcta acagcgtctg accggttggc tgaatataac ctgccctcgc ccaaaccgga
    10501 ccgtatttaa atttggctgc tgctttatac aggcttatac cgcttttatc gccggaccag
    10561 tcttcgtcat aggctttatt actttttgaa aacgcgattt cgttcgggtg agagctgtcg
    10621 ccgttttccg ccatttcaat cgccgtaaac gcggcaatat caaggccgaa catatcagca
    10681 gcataaccag actgaaaatc gaggttggcg ttccaggtgg agtgagaaag gttggttttg
    10741 tatttatcgc cgtcggtaac atctttacgg tcgcgttcac gctgccagta atagataccg
    10801 ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag cctccggaat tgccataaag
    10861 cccgacatag ctgtaacacc ggcgatagcc agcgccagcg tactacgttt gccactaaac
    10921 gcacgcatgg gttaatcctc tttgacgtat aaattgctgc accaaaggtg aacagcgaaa
    10981 aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat
    11041 ttttcgcgac gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc
    11101 gcacatattt tgatgatttt tgttacaagg cttaagtttg cagttgaaag gcttgcagaa
    11161 gaataaaaac gtg
//
LOCUS       DAZFRE010000137        11108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFRE010000137 DAZFRE010000000
VERSION     DAZFRE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.137"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            259..474
                     /gene="essD"
                     /locus_tag="ACHKUI_004452"
     CDS             259..474
                     /gene="essD"
                     /locus_tag="ACHKUI_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6600518.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            474..671
                     /locus_tag="ACHKUI_004453"
                     /pseudo
     CDS             474..671
                     /locus_tag="ACHKUI_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            654..4064
                     /locus_tag="ACHKUI_004454"
     CDS             654..4064
                     /locus_tag="ACHKUI_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6600519.1"
                     /translation="MLGKTYKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVD
                     GLKSVLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDT
                     PITRTITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIK
                     GKTTSQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDMKQCYPN
                     TALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNM
                     AWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLT
                     TQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYS
                     FSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHR
                     AGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRT
                     LTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLK
                     LPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPA
                     VQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTY
                     RFTQLALGRYTLTVRAVNAWGQQGDPASVSFRIAALAAPSRIDLTPGYFQITATPHLA
                     VYDPTVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQASDDASGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEW
                     KDANDKWNAMWGVKIEQTEDGRHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANG
                     NETPMFVAQGNQIFMNEVFLKYLTAPTITSGGNPPVFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRNGSYASGTITVTVYDDQAF
                     DRQIVIPPVLFRGGKHENFNSNNQQSYWYSTCKLQVLKNGQEIFQQPATDVSRVFSSV
                     IDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4123..6183
                     /locus_tag="ACHKUI_004455"
     CDS             4123..6183
                     /locus_tag="ACHKUI_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6600520.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEVMVEDVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            6180..6458
                     /locus_tag="ACHKUI_004456"
     CDS             6180..6458
                     /locus_tag="ACHKUI_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600521.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            6471..6761
                     /locus_tag="ACHKUI_004457"
                     /pseudo
     CDS             6471..6761
                     /locus_tag="ACHKUI_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6856..7713)
                     /gene="sitD"
                     /locus_tag="ACHKUI_004458"
     CDS             complement(6856..7713)
                     /gene="sitD"
                     /locus_tag="ACHKUI_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO6600522.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(7710..8567)
                     /gene="sitC"
                     /locus_tag="ACHKUI_004459"
     CDS             complement(7710..8567)
                     /gene="sitC"
                     /locus_tag="ACHKUI_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO6600523.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(8564..9391)
                     /gene="sitB"
                     /locus_tag="ACHKUI_004460"
     CDS             complement(8564..9391)
                     /gene="sitB"
                     /locus_tag="ACHKUI_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO6600524.1"
                     /translation="MMQSAGIVVNDVAVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(9391..10305)
                     /gene="sitA"
                     /locus_tag="ACHKUI_004461"
     CDS             complement(9391..10305)
                     /gene="sitA"
                     /locus_tag="ACHKUI_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO6600525.1"
                     /translation="MRSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         2979 a         2606 c         2953 g         2570 t
ORIGIN      
        1 gaacactcgg tggcattctc acaaatatca tttagtagtt tacgtacgta aaaaattggt
       61 tatgctgtta agagtggtta cttcgtcaca cagcttaaac ccgccgtcga gcgggttttt
      121 ccattttttg agtctcgata ttagctgata acccaatacc tgagttattc actgactccg
      181 aatctgttac gtttctgcct ttattgcgat acgtagtatc cccttaattt acacccgctt
      241 tgtctgcgag gtggggttat gaaatccatg gataagttaa caacgggtgt cgcctatggc
      301 acctcagcag gtagtgccgg gtactggttt ttacagttgc tcgataaagt cacgccctca
      361 cagtgggcgg caataggtgt gctgggtagt ctggtatttg gcttgctgac gtatctgaca
      421 aacctttatt tcaagattaa agaagataag cgtaaggctg cgagaggtga ataatgtcgc
      481 catcattacg caaggctatt gctgctgcta ttggtggtgg ggctgttgcc atagcgtctg
      541 tgctcatcac tggtccgagt ggtgacgatg gcctggaagg tgtcagctac ataccataca
      601 aagatattgt tggtgtatgg actgtatgtc acgggcatac aggaaaagac atcatgctcg
      661 gtaaaacgta taaaggcagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga
      721 aatccacgca gttactgagt gtgatcgatg ccatcagcga agggccgatt gaaggtccgg
      781 tggatgggtt aaaaagcgtg ctactgaaca gtacgccggt gctggacact gaggggaata
      841 ccaatatctc cggtgtcacg gtggtgttcc gggcaggtga gcaggagcag actccgccgg
      901 aggggtttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tacgacacgc
      961 cgatcacccg caccatcacg tcggcaaaca tcgaccgtct gcgttttacc ttcggtgtgc
     1021 aggcactggt ggaaaccact tcaaagggtg accggaatcc gtcggaagtt cgcctgctgg
     1081 ttcagataca gcgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa
     1141 ccacctcgca gtatctggcc tcggtggtgg tggataacct gcctccgcgc ccgttcaata
     1201 tccggatgcg caggatgaca ccggacagca ccacagacca gctgcagaac aaaacgctct
     1261 ggtcgtcata caccgaaatc atcgatatga aacagtgcta cccgaacacg gcactggtcg
     1321 gtgtgcaggt ggactcggag cagttcggca gtcagcaggt gagtcgtaat tatcatctgc
     1381 gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca gacgcgacaa tacagcggta
     1441 tctgggacgg aacgttaaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata
     1501 tgctgaccca tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggacaaat
     1561 gggcgctgta tgtcatcggc cagaattgcg accagtcggt gccggatggt tttggtggca
     1621 cggagccgcg catcacctgt aatgcctggc tgaccacaca gcgtaaggcg tgggatgttc
     1681 tcagtgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt
     1741 tcgtgcagga ccggccgtcg gataaggtgt ggacctataa ccgcagtaat gtggtgatgc
     1801 cggatgatgg cgcgccgttc cgctacagtt tcagcgccct gaaggaccgc cataatgccg
     1861 ttgaggtgaa ctggattgac ccggataacg gctgggagac ggcgacagag cttgtggagg
     1921 acacgcaggc cattgcccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta
     1981 ccagtcgggg gcaggcacac cgagccgggc tgtggctgat taaaacggaa ctgctggaaa
     2041 cgcagaccgt ggatttcagc gtgggcgcag aagggcttcg ccatgtgccg ggcgatgtta
     2101 ttgaaatctg cgatgatgac tatgcgggta tcagcatcgg cgggcgcgtg ctggcggtga
     2161 acagccagac ccggacgctg acgctcgacc gtgaaatcac tctgccatcc tccggtacca
     2221 cgctgataag cctggttgac ggaagcggca atccggtcag cgtggaggtc cagtccgtca
     2281 ccgacggcgt gaaggtaaaa gtgagccggg ttcctgacgg cgttgccgga tacagcgtgt
     2341 gggggctgaa gttgccgacg ttgcgccagc gcctgttccg ctgcgtgagt atccgtgaga
     2401 acgatgacgg cacgtatgcc atcactgccg tgcagcatgt accggaaaaa gaggccatcg
     2461 tggataacgg ggcgcacttt gacggcgacc agagcggcac ggtgaatggt gtcacgccgc
     2521 cagcggtgca gcacctgact gccgaagtca ccgcagacag cggggaatat caggtgctgg
     2581 cgcgctggga cacgccgaag gtggtgaagg gggtgagctt tatgcttcgc ctgaccgtgg
     2641 cagcggatga cggcagtgag cggctggtca gcacggcccg gacgacggaa accacatacc
     2701 gcttcacgca actggcgctg gggcggtaca cgctgacagt ccgggcggta aatgcgtggg
     2761 ggcagcaggg cgatccggcg tcggtatcgt tccggattgc cgcactggca gcaccgtcga
     2821 ggattgatct gacgccgggc tattttcaga taaccgccac gccgcatctt gccgtttatg
     2881 acccgacggt acagtttgaa ttctggttct cggaaaagcg gattaccgat atcaggcagg
     2941 ttgaaaccac agcccgctat cttggcacgg cgctgtactg gatagctgcc agtatcaata
     3001 ttaagccggg ccatgattat tatttttata tccgcagtgt gaatactgtt ggcaaatcgg
     3061 cattcgtgga ggctgtcggt caggcgagcg atgatgcttc cggctatctg gattttttca
     3121 aaggccagat aactgaatcc catctcggca aggagctgct ggaaaaagtc gagctgacgg
     3181 aggataacgc cagcaaactg gaggagtttt cgaaagagtg gaaagacgcc aacgataaat
     3241 ggaatgccat gtggggcgtc aaaattgagc agaccgaaga cggcaggcat tatgtcgcgg
     3301 gtattggcct cagcatggag gatacggagg aaggcaaact gagccagttc ctggttgccg
     3361 ctaaccgtat cgcgtttatt gacccggcaa acgggaatga aacgccgatg tttgtggcgc
     3421 agggcaacca gatattcatg aacgaagtgt tcctgaagta tctgacggct cccaccatta
     3481 ccagcggcgg caatccgccg gtattttccc tgacaccgga cgggcggttg acggcgaaaa
     3541 atgccgatat cagcggtaac gtgaatgcga actcagggac gctcaataat gtcacgatta
     3601 accagaactg tcggattctg ggaaaactgt ctgccaacca gattgaaggt gatattgtca
     3661 aaacggtggg aaaagccttt ccgagaaatg gcagttatgc cagcggtaca ataacggtca
     3721 ctgtgtacga tgaccaggct tttgaccgtc agatagtaat cccacccgtt ctgtttcgcg
     3781 gtggtaagca tgaaaacttc aacagcaaca accaacagtc atactggtat tcaacctgta
     3841 agctgcaggt actgaagaac ggacaggaaa tctttcagca acccgcgacg gatgtcagca
     3901 gggtattttc atccgtcatt gatatgccag cgggacacgg ccatgtcacc ctgactttca
     3961 atgtttcttc atatggtgca aataactgga cgccaacgac cagtatcagc gaccttcttg
     4021 ttgtcgtgat gaagaaatca acagccggta tcagtatcag ttgaatttta taacccaaat
     4081 acgggcgcca gaaatggcgc cttttttatt tgtggagtga atatggcagt acagatttca
     4141 ggcgtgctga aagacggtgc aggaaaacca atacagaact gtactattca gctcaaagca
     4201 aagcgtaaca gcaccacggt actggtgaac acggtggcct ctgaaaatcc ggatgaagcc
     4261 gggcgttaca gcatggatgt tgagtatggc cagtacagcg tcaccctgct ggttgaaggt
     4321 tttccgcctt cacatgccgg gaccattacc gtctatgaag gttccagacc aggtacgctg
     4381 aatgattttc tcggtgccat gacggaagat gatgtcatgc cggaggcatt gcgtcgtttt
     4441 gaggtaatgg tggaggatgt ggcacgcaac gctgaagccg cctctcagag cgcagcggca
     4501 gcaaagaaat cagaaacagc agcggcatcg tccaggaacg cggcgaaaac atcagagacg
     4561 aatgcaggta acagcgcgaa agcggcagct tcttcaaaaa cagccgcaca aaacgcagca
     4621 acagcggcag aacgttcaga gacaaatgcc cgtgcgtcag aagaagcctc cgcagacagt
     4681 gaagaggctt cccgccgtaa tgcagagtca gccgctgaaa atgccggagt cgccaccaca
     4741 aaagcgcggg aggctgcagc agacgcaaca aaggccgggc agaaaaagga tgaggctctg
     4801 tcggcagcga cacgagctga aaaggcggca gaccgcgcag aagccgcagc ggaagtgact
     4861 gcagagccct atgcgaatat agtgccgccg ctgcctgatg tgtggatacc gtttaacgat
     4921 tcactggata tgattgcggg tttttctccg ggctataaaa aaatagctat tggtgacgat
     4981 gtggttcagg tcgccagtga taaacaggtt aatttcagtc gcgcatcaac ggcaacatat
     5041 atcaacaaat ctggcgaact gaaaacggcg gaaattaatg agccgcgatt tgagtgtgat
     5101 ggcctgctta ttgagggaca aagaacgaac tacatgctca attcggaaag tccagccagc
     5161 tgggggaagt catcaaacat ggatgtgccc gaaaccggga cggatagttt tggttttact
     5221 tatggaaagt ttgtctgcaa cgattctctg gttgggcaaa cttcggctat taatatggca
     5281 tcaattgctg caacaaagtc agttgatgtc tcaggcgata acaagtacgt gacaacctca
     5341 tgccgtttta aaacagaacg acaggtaagg ttacgtatac ggtttgataa gtatgatggt
     5401 agtgcaacaa cttttcttgg cgatgcgtac attgatacgc aaacgcttga aattaatatg
     5461 acaggtggtg ctgccggcag aattacggca cgagtcagga aggataagac cacgggctgg
     5521 atttttgcag aggcaacgat tcaggcaatt gatggtgagt taaaaatagg ctctcagata
     5581 cagtattctc ctgagcaggg tggggcaaca gtatctggtg actatattta tcttgccacc
     5641 ccacaagtag agaatgggcc gtgtgtatca tcatttatta tttcaggagg cagcgcaacg
     5701 acaagagcca gtgatttggt tagtatcccc accagaaata atctttataa gttaccattt
     5761 acttttttac ttgagattca taaaaactgg gatattgcac caaacgccgc accccgcgtg
     5821 tgggatatag cagcagccaa taccgggcaa tcagcaattg cagcaatcaa cagaggtagt
     5881 ggtaagttat atatgagtct gtcaaaccct tcaggctcgt atgttaatag cgcagcgaca
     5941 gatgtatttg cagagaaaac cacatttgga tgtattgcaa aagctgatgg tcactttcat
     6001 gtggtgacaa atggtaaagc ggttaatgaa gtttattgtg aatataatgg cgtgaccgct
     6061 gataaaaata tccgatttgg agggcagacg aatactggag aacgacatct gtttggccat
     6121 attcgcaatt tccgcatatg gcataaagaa ttaaatgaca ggcaattaaa agaggtcgta
     6181 tgaaagattt aactttgaag tttcatgaca aactgcagtt taaggccttc ctgtcatctc
     6241 ttggctgggc ggaagatgaa gacctccaga ataaactgtt agttgatgaa attggtttca
     6301 cctacacaga aacaggggta acagaagagg gagaacctgt ctgtatccgg aatgatggtt
     6361 attttgtcaa cattcgcatt cttgatgact tgtttgatgt ttctgtattc tctgattatg
     6421 tcgtggagct ggaaacaccg cttcgggaat ggagctgaaa ggaggaaata atggatataa
     6481 gccccttact tcatgcactt tgtgctgtgg ctgcgcagat actggttggt ctttttaccg
     6541 gaaactgggc ttacggagcg atagccggtt gtacgttctt cattgcgcgt gaacataccc
     6601 aggcagaata tcgctggatt gaaatgttcg ggcatggcaa gcgaatgaat atgccgtggt
     6661 ggggcggttt tgatccgcgt gcttgggatg tggcaagcct gatggatttt gctgtgccgg
     6721 tggtggcgtg tctgctggtc tggctgttgg ttaatcgtgg gcgaaaaaag gtgagctgta
     6781 tatgcaacgg aggaagaaac cccgttgctg gaagccagga aaaagtatcg ggtgttgctg
     6841 tcctcggatg ttgtgctaat tgggtgataa cagatcctgt gtatgggagt ttcccttttt
     6901 gcgtgcgttg atgctggtaa cggcaaacgt catgatgaac aaaaccgcga ataagacgac
     6961 aatcgtcgga gctggtgcgc tgtcgataaa aaaggacaac cagacaccag tcattgacac
     7021 tattaccgat atgccagtcg ccagtaggag cgcaatatga aagcgttgtg tcaggagtac
     7081 cgcaatggcg ccaggcgcaa tgagcagaga aatggagaga atgataccta ccgctttcag
     7141 cgtcgccaca atggtcagag agaccatgca cagcagccca taatgcagcc atctcgtatg
     7201 caaaccgctt acctgcgcct gttgataatc aaagctgaac agcaaaaaat cccgccattt
     7261 tacgctaata acaagtgtaa ccagcccggc aataatcatc gtctggatta tatcgccgat
     7321 ggttatcccg agcatgtcgc cgaaaagaat atggtcaaga tgtacgtctg gtttgactgc
     7381 aatatacaaa atcagacctg cggcaaacat gccagaaaaa acaatcccca tcactgtatc
     7441 ttgcttgatt cggctgttgt ctttcaggta tcccgtcgcc accgcgcaga atactccggc
     7501 gacgaatgcc cctgtagcca aaggtaaccc cagaatccag gcaagcacga tgccaggaaa
     7561 aaccgcatga ctcatcgcgt cacccatcag tgcccagcct ttcaggacca gaaagaccga
     7621 cagcagcgca cagggtatgg aaactaccag tgaaatcagc agtgcgtgac tcataaaggt
     7681 gaactgcagt ggctccagta ataatgccat catgagcgat cctccagcgc cttatgcgca
     7741 cgccgacgat ttgccaaaag cccatgggtg ggcgcaaaga caaatgctaa aagaaacagc
     7801 agtgtctgcg ccaccacgat gattccacct gtcgcgccgt ccagaaagta actgacccac
     7861 gcgccgagga aactggttac actgccaata gttacagcaa tcatgagcag gcggggaaaa
     7921 cgatcggtta acaaccatgc tgttgctccg ggagtcacca ccagacagat caccagaaag
     7981 gcaccaaccg tttgcagcgc cgcaacggtc gaaaccgaca gcagggtgaa gaagaggagt
     8041 tttaatctgc ccggatgcaa tccaatagca cgggcgtgat tctcgtcgaa aaaggtcacc
     8101 ataagatctt tccatttgaa aaaaagcacg atgatcgaga gaatgccaat gatagtgagt
     8161 tgcaggatat ccgccggatc gattgccaga atattgccca ggacgatggt ctgaatgttc
     8221 acagatgtcg ggttcaatga caccataaac agccccaggc caaaaaagga cgagaagatc
     8281 aggccgatga tggcatcttc ttttaagcgg gtgcgttggt taagaaacag catgctgccc
     8341 gcagccagtc cgccggaaaa gaaggctccc agagaaaacg gaagccccag catatatgcc
     8401 cccgcaacac ccgggacaat agagtgcgac aacgcatcgc caatcagcga ccagcctttt
     8461 agcatcagat agcatgacag gaaagcgcag agaccgccga ccatcgccga gacccacatc
     8521 gcattgagca tatattcata gctgaagggt tccagtagca cgttcatctt tcttccctct
     8581 gcaccgctga cggtcgatga gccacaaaag ggcgttcatc gtcagtaata atgctttctt
     8641 ccgagccgtt aagtgtgaca tggcgcagta cgccgctaaa agccagctcc aggttctcgg
     8701 ctgtaaaagt ggtgtctgtc ggcccgcttg ccaggacggt gcctttgacc atgaccgtat
     8761 aatcgcaaaa tgtcgtgacg gatccgagat tgtgcgttga gacaagcatc gttttgcctt
     8821 ccgcgcgtaa ctccctcaac aggctgatga ttttggcttc ggttttcaca tcaacgccag
     8881 taaaaggttc atccagcagg atcacatcac cctgctgtgc aatcgctctc gccagaaaga
     8941 cgcgtttctt ttgtccaccg gaaagctcgc cgatttgtcg atggcgaaaa ttcaccatat
     9001 cgacacgttc cagcgcatcg gtgacaattt gacgatccct ctttttggcg atacgcaaca
     9061 tacccatatg cccatagcgg cccatcatca ccacatcttc caccagtaca ggaaatgacc
     9121 agtcaacctc ttctgactga gggacgtagg caaccaggtt tttctgtaac gcctgtcgcg
     9181 tgggaatacc cagaacagat atctttccgc tggtcagacg cacaaatccc ataatcgctt
     9241 taaacaacgt cgattttccg gaaccgttta ccccaaccag agcggcaata gatccacttg
     9301 gtactgtgaa ggatgcatcg cgcagtgctg tgtgcccgtt acgccaggtg acagcaacat
     9361 cattaacgac aatgcctgca gattgcatca ttatttctcc cttttcccgg ctttaattcc
     9421 ctgtaccagc gtactggtag taaccttaag aaggtcgata tacgtcggta ccgggccgtt
     9481 ttctgtgctc agggaatcga catagagcac accgccgtag tgtgcgccgg tttcacgcgc
     9541 aacctgacgc gctggtttat cggaaatcgt actctcgcta aagactgccg ggatatgatt
     9601 tttcttaact atatcaacaa ccttacgtac ctgctgcggt gttccttgtt gatcggcatt
     9661 aatcggccac agataaagct cttttagccc caaatcgcgt gcgagataag aaaaagcccc
     9721 ttcactggtg accatccatc gctgattctc aggaagttcc gtaatctgct tacgcagggg
     9781 ggcaagggtt tgggtaatct tggctttata agtatcggca ttgcgttggt aggtttgtgc
     9841 atttgccgga tcgtatttta tcaacgcatc acgaatatta tcgacgtaaa tcagagcatt
     9901 atctggcgac atccatgcat gggggttagg tttgccctca tagggtcctt cggtgatccc
     9961 tacaggcgtc acacccgaag agacaattac ttctggaacc ccattgagat gctggtaaaa
    10021 gcgttggaac cacaattcca gattcatacc attggcgaga atcagttgtg ccccctgcgc
    10081 acgtttaata tcgccagggg taggctgata ctcatgaatt tctgcaccag gcttggttat
    10141 ggatgagact tctgcagcat ctcccgccac gtttttggcc atatccgcga tgatggtaaa
    10201 tgttgtaatg accttaaatt tttcagttgc acttgcctga aatgcgatcg agcaggtgag
    10261 tgcgaggccc cccaagagca tggttacttt ttttattgag cgcataattt agtacctata
    10321 gtgagcaaaa ttagaacaaa catagcaacg gctatgtttt atagcataac ctatacagaa
    10381 tgaaaatgat tatcattaac atctttgagg cccaaaacag ggattagttt gtttcctgct
    10441 ggatgaaagg actcttggga tagcacatgc cgcacgaaga gcagcattac atgatgtttt
    10501 gtcgaatgag ctacgaatcc tgatagaaat acgaaacaag ctagcacatg gacaatggat
    10561 ctatcccttc aataatgaag gaaatactgt ggaccctgat aaatataaat taatcaataa
    10621 agaaaacttg cagtcactcc aattcaaata ttcattagca cagcatttag ttgacgctgt
    10681 acatgatttg gttgtaagtc ctacgacatt cgaacgagat ttcgaaagcc atttttaaaa
    10741 attatatcaa gtacgaacaa acttagtgac aaaagactac gaaaaatata aaacaatttt
    10801 agtattaagt agagagaaat ttcgttctgc taggacgcag caataggttc atgagattct
    10861 ctgatacttg ttaccttagt aagccttaaa tttttaaaaa ttcttttacg tccactcctc
    10921 gctcataaca ggcattaact acatttatct tcattggcta tgcaagtcgt aataaaaggt
    10981 gggacaaaac tgagacacat aagctctcac aaaggtctgc aaggcctcac atgtattgag
    11041 atgttaataa aatgtagact tgtaattttg atataaatgg tagataaaac ctttccccaa
    11101 aataaaaa
//
LOCUS       DAZFRE010000138        10884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFRE010000138 DAZFRE010000000
VERSION     DAZFRE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.138"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1125
                     /gene="yghO"
                     /locus_tag="ACHKUI_004462"
     CDS             <1..1125
                     /gene="yghO"
                     /locus_tag="ACHKUI_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO6600526.1"
                     /translation="MIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSA
                     KNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDDPQVFAA
                     LFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQL
                     GYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNS
                     GWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADL
                     NGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFAR
                     RKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1132..1896)
                     /gene="glcC"
                     /locus_tag="ACHKUI_004463"
     CDS             complement(1132..1896)
                     /gene="glcC"
                     /locus_tag="ACHKUI_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO6600527.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2147..3646
                     /gene="glcD"
                     /locus_tag="ACHKUI_004464"
     CDS             2147..3646
                     /gene="glcD"
                     /locus_tag="ACHKUI_004464"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO6600528.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNQAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPMARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3646..4698
                     /gene="glcE"
                     /locus_tag="ACHKUI_004465"
     CDS             3646..4698
                     /gene="glcE"
                     /locus_tag="ACHKUI_004465"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO6600529.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4709..5932
                     /gene="glcF"
                     /locus_tag="ACHKUI_004466"
     CDS             4709..5932
                     /gene="glcF"
                     /locus_tag="ACHKUI_004466"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO6600530.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5937..6341
                     /locus_tag="ACHKUI_004467"
     CDS             5937..6341
                     /locus_tag="ACHKUI_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO6600531.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6363..8534
                     /gene="glcB"
                     /locus_tag="ACHKUI_004468"
     CDS             6363..8534
                     /gene="glcB"
                     /locus_tag="ACHKUI_004468"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO6600532.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHSILTKEQVQASLENMAKVVDQQNAGDPAYRPMSG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8889..10571
                     /gene="glcA"
                     /locus_tag="ACHKUI_004469"
     CDS             8889..10571
                     /gene="glcA"
                     /locus_tag="ACHKUI_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO6600533.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGMGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10705..>10884)
                     /locus_tag="ACHKUI_004470"
     CDS             complement(10705..>10884)
                     /locus_tag="ACHKUI_004470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600534.1"
                     /translation="RNFNKQKINNFSQRNYINLLVVLTIKIAKATDRTVFAHQANIIN
                     TDLFCVIKKQMFSRF"
BASE COUNT         2535 a         2720 c         3059 g         2570 t
ORIGIN      
        1 atgattaaaa ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg
       61 tcatcacttt atcccgacga tccaaactgg ataccccctt tattcattga acgcaacgag
      121 catttgtctg cgaaaaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa
      181 aaagcggggc agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgcgagcgt
      241 tacggcaaag ataccggtca tttcggcatg attgacgcca ttgatgatcc gcaggttttt
      301 gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag taagatcagc
      361 ggtcctttca gcctgaatat caatcaggaa agcggattac tgattgaagg tttcgataca
      421 ccaccctgtg cgatgatgcc gcacggtaaa ccgtggtatg ccgcgcatat tgaacaattg
      481 ggttatcaca aaggcattga tttactggcg tggtggatgc agcgaaccga tctcactttc
      541 tctccggcgc taaaaaaact gatggatcag gtgcgtaaga aggtgaccat tcgctgcatc
      601 aatcgccagc ggtttgccga agagatgcag atcctgcgtg agattttcaa ttctggctgg
      661 cagcacaact ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgaccaa
      721 cttaaatatc tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc
      781 gcgtttattg tcggcttgcc aaacatcaac gaggcgattg ccgatctgaa cggatcgctc
      841 tttcccttcg gctgggcaaa attgctgtgg cgtttaaaag tcagcggtgt gcgaactgcg
      901 cgagtaccgc tgatgggcgt gcgggacgag tatcagttca gccgcatcgg cccggtgatt
      961 gcgttgttat tgattgaagc cttacgcgat ccgtttgccc gccggaagat cgatgcgctg
     1021 gagatgtcat ggatcctcga aaccaataca ggcatgaata acatgctgga acgcattggg
     1081 gcggagccgt acaagcgtta ccgattgtat gaaaagcaga tttgatggca gtcacccctg
     1141 cttcatctcc agcggcaccg ccgagcgaat caaatggtgg ccttccagct cgatatcgtg
     1201 gagattcttt ttcacggtcc gcacatgatc gcgtgctgcg cgctgggcga cgtgcggcag
     1261 ccgctgcaac accgcgttgt agatccgcgc atgctggcga tcgatctgct ttttttgctg
     1321 tggtcgatgg tagagattat ttacgctggc aaacaccgaa ttaaacatca ggtcggtcaa
     1381 tgattgcagc gtaaactcca gcacctgatt gtgagaagcc tgacaaatgg cgagatggaa
     1441 agcgtgatcc aactgcgcat gttcgatcag cgaaatctct ttgttgttct cactggcggc
     1501 gagcattttt tcataacagc gggttatcac aacaaaatca gcctgggttc ccagcgttgc
     1561 cgccagcctt gccgattcgc cctccagtaa tgcgcgaacg tcgagcagat cgtacagcgt
     1621 tcgcggctgc gtactgaaca gatggatcag cgggctggtg tcctgcaccc gattaagccg
     1681 tgcgacacga gaatcgcgac cctgcgccgt ttcaataatc ccgcgcccgc gcagcacggt
     1741 cagcccttca cgcagtgcgg agcgtgagaa gccgagcttt tcacacagtc gacgttccga
     1801 gggaagcggc tgaccgacct tcagtacgcc gtcgataatt aaccgttcga tactctctgc
     1861 aaccacttcg caaatagggc gacgttcatc tttcattccc ggacctcgtg cacaggtagg
     1921 accaattttt ctgttcttaa ctcgcaaaac acgcacatca cgtaagtgat tatgttacat
     1981 caatttaaca ttaagttaac caagacaagg tcacagagct ggaaaaaaaa tggtctgacc
     2041 ggtagctaaa gagatagacg aaaacgaaaa gcccgcttaa taactgttca cagaagcagc
     2101 gcgaaaaaat cagctgccac acaacacaac aaagcgaagc ctactcatga gcatcttgta
     2161 cgaagagcgt cttgatggcg ctttacccga tgtcgaccgc acatcggtac tgatggcact
     2221 gcgtgagcat gtccctggac ttgagatcct tcataccgat gaggagatca ttccttacga
     2281 gtgtgacggg ttgagcgcgt atcgcacgcg tccattactg gttgttctgc ctaagcagat
     2341 ggaacaggtg acggcgattc tggctgtctg ccaccgcctg cgtgtaccgg tggtgacccg
     2401 tggtgcaggc accgggcttt ctggtggcgc gctgccgctg gaaaaaggtg tgttgttggt
     2461 gatggcgcgc tttaaagaga tcctcgacat taaccccgtt ggtcgccgtg cgcgcgtgca
     2521 gccgggcgtg cgtaaccagg cgatctccca ggccgttgcg ccgcataatc tctattacgc
     2581 gccggatccg tcctcacaaa tcgcctgttc gattggcggc aatgtggcgg aaaatgccgg
     2641 tggcgttcac tgcctgaaat atggtctaac cgtacataac ctgctgaaaa ttgaagtgca
     2701 aacgctggat ggcgaggcgc tgacgctggg atcggacgcg ctggattcgc ctggttttga
     2761 cctgctggcg ctgtttaccg gatcggaagg tatgctcggc gtgaccaccg aagtgacgtt
     2821 aaaactgctg ccgaagccgc ccatggcgcg ggtgctgtta gccagctttg actcggtaga
     2881 aaaagccgga cttgcggttg gtgacatcat cgccaatggc attatccccg gcgggctgga
     2941 gatgatggat aacctgtcga tccgcgctgc ggaagatttt atacatgccg gttatcccgt
     3001 cgatgccgaa gcgattttat tgtgcgagct ggacggcgtg gagtctgacg tacaggaaga
     3061 ctgcgagcgg gttaacgaca tcttgttgaa agcgggcgcg actgacgtcc gtctggcaca
     3121 ggacgaagca gagcgcgtac gtttctgggc cggccgcaag aacgcattcc cggcagtggg
     3181 gcgcatttcc ccggattact actgtatgga tggcaccatc ccacgccgcg cgctgcctgg
     3241 cgtactggaa ggcattgccc gtttatcgca gcaatatgat ttacgcgttg ccaacgtctt
     3301 tcatgccgga gacggcaaca tgcacccgtt aatccttttc gatgccaacg aacccggtga
     3361 atttgcccgc gcggaagagc tgggcgggaa gatcctcgaa ctctgcgttg aagttggcgg
     3421 cagcatcagt ggggaacatg gcattgggcg cgaaaaaatc aatcaaatgt gcgcccagtt
     3481 caacagcgat gaaatcacga ccttccatgc ggtcaaggcg gcgtttgacc ccgatggttt
     3541 gctgaaccct gggaaaaaca ttcccacgct acaccgctgt gctgaatttg gtgccatgca
     3601 tgtgcatcac ggtcatttac ctttccctga actggagcgt ttctgatgct acgcgagtgt
     3661 gattacagcc aggcgctgct ggagcaggtg aatcaggcga ttagcgataa aacgccgctg
     3721 gtgattcagg gcagcaatag caaagccttt ttaggtcgtc ctgtcaccgg gcaaacgctg
     3781 gatgtgcgtt gtcatcgcgg cattgttaat tacgacccga ccgagctggt gataaccgct
     3841 cgtgccggaa cgccgctggt ggcgattgaa gcggcgctgg agagcgccgg gcaaatgctc
     3901 ccctgcgagc cgccacatta tggtgaagaa gctacctggg gcgggatgat cgcctgcggg
     3961 ctggcggggc cgcgtcgccc gtggagtggt tcggtccgcg attttgtcct cggcacgcgc
     4021 atcattaccg gcactggaaa acatctgcgt tttggtggtg aagtgatgaa aaacgttgcc
     4081 ggatacgatc tctcacggtt aatggccgga agctacggtt gtctgggtgt gctcactgaa
     4141 atctcgatga aagtgttacc gcgaccacgc gccaccctga gtctgcgtcg ggaaatcagc
     4201 ctgcaagaag ccatgaatga aatcgcccag tggcaactcc agccattacc cattagtggc
     4261 ttatgttact tcgacaatgc gttgtggatc cgccttgagg gcggcgaagg atcggtaaaa
     4321 gcagcgcgtg aactgctggg gggcgaagag gttgcgggtc agttctggca gcaattgcgt
     4381 gaacaacaac tgccgttctt ctcgttacca ggtaccttat ggcgcatttc cttacccagc
     4441 gatgcgccga tgatggattt acccggcgag caactgatcg actggggcgg ggcgttacgc
     4501 tggctgaaat cgacagccga tgacaatcaa atccatcgca ttgcccgcaa cgctggcggt
     4561 catgcgaccc gctttagtgc cggagatggt ggttttgccc cgctaccggc tcctttattc
     4621 cgctatcacc agcagcttaa acagcagctc gacccttgcg gcgtgtttaa tcccggtcgc
     4681 atgtacgcgg agctttaagg agcaggctat gcaaacccaa ttaaccgaag agatgcggca
     4741 gaacgcgcgg gcgctggaag ccgacagcat cctgcgcgcc tgtgttcact gcggattttg
     4801 taccgcaacc tgcccaacct atcagctcct gggcgatgaa ctggacgggc cgcgcgggcg
     4861 catctatctg attaaacagg tgctggaagg caacgacgtc acgcttaaaa cacaggagca
     4921 tctcgatcgc tgtctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta
     4981 tcacaatttg ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc
     5041 ggagcgaatg ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc
     5101 gctgacgcag gtagggctgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact
     5161 gcctgctgaa acggtgaaag ctaaaccgcg tccaccgctg cgccataagc gtcgggtttt
     5221 aatgctggaa ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg
     5281 agtgctggat cgtctgggga tcagcgtcat gccagctaac gaagcaggct gttgtggcgc
     5341 ggtggactat catcttaatg cgcaagagaa agggttagcg cgggcgcgca ataatattga
     5401 tgcctggtgg cccgcgattg aagcaggtgc cgaggctatt ttgcagactg ccagcggctg
     5461 cggcgcgttt gtcaaagagt atgggcagat gctgaaaaac gatgcgttgt atgccgataa
     5521 agcgcgtcag gtcagtgagc tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct
     5581 ggaaaaactg gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca
     5641 acatgcgcaa aagctgaacg gcgaagtgga aaaagtgttg cttcgtcttg gatttacctt
     5701 aacggacgtt cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca
     5761 tcccgatctg gcgcgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc
     5821 ggaaatgatc gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccggtcgtac
     5881 ctctgtgcgt cactggattg aaattgtaga acaagccctt gaaaaggaat aacaaaatga
     5941 aaactaaagt cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag
     6001 aggcgcagaa aaacaactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc
     6061 tggcgttaag tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag
     6121 cgcgtaccgc cgcgctgggg cgtcgtgaaa ctaaggggta tgaagagatg gtgaacaacg
     6181 gacgtaccgc gttcgtgact gcgccgttat taacgtcgct agaaggtggc gtaccggttg
     6241 ttgtggatgg gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg
     6301 cgcaggtcgc gaaagcggca gcagcggtgt tggcgaaata agcgaaaacg aggagagaaa
     6361 caatgagtca aaccataacc cagggccgtt tacgcattaa cgccaatttt aaacgttttg
     6421 tggatgaaga agttttaccg ggaacagggt tggacgctgc ggcgttctgg cgcaattttg
     6481 atgagatcgt tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca
     6541 ttcaggcggc gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg
     6601 cctataaatc tttcctgcgt gaactgggct acctggtgcc gcaaccggag cgcgtaacgg
     6661 tggaaaccac gggcattgac agcgaaatca ccagtcaggc ggggccacag ctggtggttc
     6721 cggcaatgaa cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg
     6781 atgcgttata cggcagcgac atcatcccgc aggaaggggc gatggtcagc ggctacgatc
     6841 cgcagcgtgg tgagcaggtt atcgcctggg ttcggcgttt cctcgatgaa tcactaccgc
     6901 tggaaaacgg cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca
     6961 tccagttgaa aaatggtaaa gaaaccacgt tacgtacccc ggcgcagttt gtcggttacc
     7021 gtggcgatgc cgctgcgccg acctgcattt tgctgaaaaa taacggtctg catattgaac
     7081 tgcaaattga tgccaacggg cggattggca aagacgatcc ggcgcatatc aacgatgtta
     7141 tcgtcgaagc ggccatcagt accattctcg actgcgaaga ttcggtcgcg gcggttgatg
     7201 cggaagataa aatcctgctg taccgcaacc tgctgggcct gatgcagggg actctgcaag
     7261 agaaaatgga aaaaaacggt cggcaaatcg tgcgtaaact gaatgacgat cgtcattaca
     7321 ccgccgccga tggctctgaa atttctctgc acggacgctc gctgctgttt atccgcaacg
     7381 tgggtcattt gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag
     7441 gcattcttga tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa
     7501 actcgcgcac tggcagcgtc tatattgtga aaccgaaaat gcacggtccg caggaagtgg
     7561 cgttcgccaa caaactgttt acccgcattg agacaatgct cggtatggca ccgaataccc
     7621 tgaaaatggg cattatggat gaagaacgtc ggacctcgct gaacttgcgt agctgtatcg
     7681 ctcaggcgcg taaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg
     7741 aaatgcattc ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc
     7801 cgtggatcaa agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctgcgcg
     7861 gtaaagcgca aatcggtaaa gggatgtggg caatgccgga cctgatggca gacatgtaca
     7921 gccagaaggg cgaccaactg cgtgccgggg caaacacagc ctgggttccg tcaccaaccg
     7981 ctgctacgct ccatgcgctg cactaccacc aaaccaacgt acagagcgtg caggccaaca
     8041 ttgcccagag cgagttcaat gctgaatttg aaccgctgct ggacgatctg ctgactattc
     8101 cggtggctga aaacgctaac tggtcggttg aagagatcca acaagagctg gataacaacg
     8161 tgcaggggat tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag
     8221 tgccggatat tcacaacgtg gcgctgatgg aagaccgcgc aacgctgcgt atctccagcc
     8281 agcatatcgc caactggtta cgtcacagta ttctgaccaa agaacaggtg caggcgtcgc
     8341 tggagaatat ggcgaaagtg gttgatcagc aaaacgctgg cgatccggct tatcgtccga
     8401 tgtcggggaa tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta atcttcctcg
     8461 gcgtgaaaca gccaaacggc tataccgaac cgttattaca cgcctggcgt ttacgcgaaa
     8521 aagaaagtca ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg ggctatgata
     8581 gtaactgcgg tagtacattt atcccggaaa taaccggagc gattatcagg gtttaacaat
     8641 attaaatacc cgtagcgcat tactagcggg cctaaaacaa caatatggac atcttaacga
     8701 tattctgcgc ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt tatgccagag
     8761 gtagcaattg cgttagcagc gtactgttgc tgtaaagtat aacgcctata ttttacagtt
     8821 cagtcctctt ttgcttatta caaccttaag tatatcaagc atataaagat aataagagac
     8881 tgaaaaatat ggttacctgg acccaaatgt atatgccgat gggaggactg gggctatccg
     8941 ctctggtcgc cctgatcccg ataatattct tcttcgttgc actcgcggta ttacgtctga
     9001 aaggacatgt cgccggagca ataaccctta tattatctat tctgattgcg atattcgcct
     9061 ttaaaatgcc gattgatatg gcatttgctg ctgcgggcta tggctttatt tatggattat
     9121 ggcctattgc gtggattatt gttgcggcgg tgttcctcta taaattaacc gttgccagcg
     9181 gtcagttcga tattatccgc agctcggtta tctccatcac tgacgatcag cgtttgcagg
     9241 tgttactgat tggtttctcc tttggcgcgc tgctggaagg ggcggctggc tttggtgcgc
     9301 cggtggcgat taccggtgcg ctgctggtgg gcctgggctt caaaccgtta tacgcggcgg
     9361 ggctgtgtct gattgccaac actgcgccgg tggcgtttgg tgcgttgggc gtgccgattc
     9421 tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat tggcgcaatg gcgggacgtc
     9481 agttaccgtt cctgtcggtt cttgtgccgt tctggctggt agcaatgatg gacggctgga
     9541 aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg gggaagcttc gctgtcacgc
     9601 agttctttac ctctaactat attggtccgg aactgccgga tattacttcg gcgctggtga
     9661 gtatcgtctc actcgcttta ttccttaaag tctggcggcc gaaaaatacc gaaacggcaa
     9721 tcagcatggg acaatccgca ggtgcgatgg tggtgaataa accatcttct ggcggtcccg
     9781 tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg gtcaccgttt ttaatcttaa
     9841 cggtgctggt caccatctgg accatgaagc cgtttaaagc gttatttgct ccgggcggcg
     9901 cgttttattc actggtgatt aatttccaga tccctcattt gcatcaacaa gtgttgaaag
     9961 cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt gtttaaattc gaccctctct
    10021 cggctggcgg caccgctatt tttattgcgg cgattatctc tatcttcatc ctcggcatgg
    10081 gcatcaagaa agggattggc gtctttgccg aaacgctaat tagtctgaag tggccgatat
    10141 tgtcgattgg catggtgctg gcgttcgcct tcgtcaccaa ctattccggc atgtccacca
    10201 cgctggcgct ggtactggca ggtacaggcg tgatgttccc gtttttctca ccgtttctcg
    10261 gctggctggg cgtattcctt accggctcgg acacctcctc taacgccctg tttggttcac
    10321 tgcaatcgac cacggcgcag caaatcaacg tctctgacac tctgctggtg gcagcaaata
    10381 ccagcggcgg cgtaactggc aagatgattt ccccgcaatc tatcgccgtg gcctgcgccg
    10441 cgacgggcat ggtgggccga gaatctgaac tgttccgcta caccgtgaag cacagtctga
    10501 tctttgccag tgtgatcggc attatcacgc tgttgcaggc gtatgtgttt accgggatgt
    10561 tagtttcgta agttaataaa gccggatgat cgttaatatc atccggcttc gtttacatta
    10621 ctctgtctta ttaatggaaa attatttccc gctttattat ccatgtggca ataatgtttc
    10681 gcgttcagat aacctgctaa ataattaaaa gcgtgagaac atctgttttt ttatcacgca
    10741 aaacaagtca gtattaatta tgttcgcttg atgagcaaac accgttctat ctgttgcttt
    10801 tgcaatctta atcgttaaaa ctaccaacaa atttatatag ttacgttgtg aaaagttgtt
    10861 aattttttgt ttattgaaat ttcg
//
LOCUS       DAZFRE010000139        10694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFRE010000139 DAZFRE010000000
VERSION     DAZFRE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.139"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..377
                     /locus_tag="ACHKUI_004471"
                     /pseudo
     CDS             1..377
                     /locus_tag="ACHKUI_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615349.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            460..717
                     /locus_tag="ACHKUI_004472"
     CDS             460..717
                     /locus_tag="ACHKUI_004472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600535.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1274..2041)
                     /gene="fecE"
                     /locus_tag="ACHKUI_004473"
     CDS             complement(1274..2041)
                     /gene="fecE"
                     /locus_tag="ACHKUI_004473"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO6600536.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGSTVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2042..2998)
                     /gene="fecD"
                     /locus_tag="ACHKUI_004474"
     CDS             complement(2042..2998)
                     /gene="fecD"
                     /locus_tag="ACHKUI_004474"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO6600537.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2995..3993)
                     /gene="fecC"
                     /locus_tag="ACHKUI_004475"
     CDS             complement(2995..3993)
                     /gene="fecC"
                     /locus_tag="ACHKUI_004475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO6600538.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3990..4892)
                     /gene="fecB"
                     /locus_tag="ACHKUI_004476"
     CDS             complement(3990..4892)
                     /gene="fecB"
                     /locus_tag="ACHKUI_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO6600539.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4937..7261)
                     /gene="fecA"
                     /locus_tag="ACHKUI_004477"
     CDS             complement(4937..7261)
                     /gene="fecA"
                     /locus_tag="ACHKUI_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO6600540.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7348..8301)
                     /gene="fecR"
                     /locus_tag="ACHKUI_004478"
     CDS             complement(7348..8301)
                     /gene="fecR"
                     /locus_tag="ACHKUI_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO6600541.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8298..8819)
                     /gene="fecI"
                     /locus_tag="ACHKUI_004479"
     CDS             complement(8298..8819)
                     /gene="fecI"
                     /locus_tag="ACHKUI_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO6600542.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8956..9024
                     /gene="ykiE"
                     /locus_tag="ACHKUI_004480"
     CDS             8956..9024
                     /gene="ykiE"
                     /locus_tag="ACHKUI_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO6600543.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            9111..9808
                     /locus_tag="ACHKUI_004481"
                     /pseudo
     CDS             9111..9808
                     /locus_tag="ACHKUI_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(9819..9890)
                     /locus_tag="ACHKUI_004482"
                     /pseudo
     CDS             complement(9819..9890)
                     /locus_tag="ACHKUI_004482"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9950..10081
                     /locus_tag="ACHKUI_004483"
                     /pseudo
     CDS             9950..10081
                     /locus_tag="ACHKUI_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612152.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10126..10230
                     /locus_tag="ACHKUI_004484"
                     /pseudo
     CDS             10126..10230
                     /locus_tag="ACHKUI_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2472 a         2849 c         2963 g         2410 t
ORIGIN      
        1 accatgaaag cactggaaat ggcatgggaa acccgtggta agcccgtcgg ggtgatgttc
       61 caagcgatca aggcagtcat tatacgagca ggcagttccg gcagttactg tggcgatacc
      121 ggatcaggca gagtatgagt cggcgtggaa actgctggga taacagccca atggagcgct
      181 tcttcaggag tctgaagaac gaatgggtgc cagcgacggg ctatgtaagc ttcagcgatg
      241 cagctcacgc aataacggac tatatcgttg gatattacag cgcactaaga ccgcacgaat
      301 ataatggtgg gttaccacca aacgaatcag aaaaccgata ctggaaaaac tctaacgcgg
      361 aggccagttt tagttgacca caacagacta cctgaaggga gccgcggtcg cctggcagtt
      421 gcagtagcag gagatcatcc agccgcagta caggtcacga tgactctggt taatgatacc
      481 ggctttgacc ccgtattttc cggctctatc gctgaatcat ggcgtcagca gccgtgcaca
      541 ccatcctatt gttgtgactg ggaggctgcc accatgcttc gcgctttccc tctggcgaaa
      601 aagggagaag gacgggcccg tctgccttca ctttatgcca gcttcggtaa gctgggtgag
      661 acaccgactc atgaagatat cattgataac aatcgatcca tcaactggcc tgtataacgt
      721 ggctgccggt gattaagaaa gctgcaccta cctaagtagt agcaaacgca cactttttag
      781 aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt ttgatttacg
      841 ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat gaaccagctc
      901 cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccctga aatctaaact
      961 tagtcatgtc acgtttttgg gtttctaaaa ttttaacttc gcgtttttcg ttgccgtaag
     1021 ggttatacag aaatgtccgt taagcagagt tcaaaattga ttgccgtgat cacgactggt
     1081 ttgaaagccg cgcccaagcc tgtacagctc tggtttgcgt tgattatgaa cctgtcagcc
     1141 taaagcaagc ggatggacga tgagtattgg taatctttca gagtccggaa aagttcagcc
     1201 ccagtctgaa caggcttgct ggcgccagtc cagtttcatt cagtcgtggt ttggttctta
     1261 cggcctgtgc aatctacctc attaggcaca tcggcctgcc agataccggc tcggggtgta
     1321 tttccgcttc cacgctgaat actgttctca gcaatcctgg ggtcatcacc tcttctggtg
     1381 tgccttgcgc cataacatgt ccgtttgcca ttaccaccag ttgatcgcag taccggctag
     1441 cctgattaag gtcgtgcagc acagcgacca ccgttttccc ctgagtccgg agttcgccca
     1501 tcaaccgcat caggtccacc tggtgattga tatcaagata ggtggttggc tcatcaagta
     1561 atacaacggg cgtattctgg gccaggacca ttgccagaaa tgcgcgctgg cgctgaccgc
     1621 cggaaagctc ggttaaccga cgaacggcaa gatgattgat ccgggtctgg ttcatggcga
     1681 cattaactcg tgcattgtct tcagcggaga gacgccccca gagtgacagc cagggattac
     1741 gaccatacga aaccagctcc tggactgtgc tcccctctgg cgttaaatgg tgctgaggca
     1801 gcagcgaaag cctgcgggcc aactggcgcg atgagagcat atttatggga ttatcgccga
     1861 gaaatacggt gccagactgc ggcattaaaa gccgcgaaaa acagtttaac agcgtcgatt
     1921 tcccgcaacc gttaggaccg atcagggcgg tgatcttccc cgttggcagt gagagtgaaa
     1981 cgtcgttaag taccttgtct gtcccgtaac tgaccgtcag attttcagtt cgtaaagtca
     2041 tttatcgcat tctcacaagc aaccagacaa accacggcgc accgataatg gcggtcagca
     2101 cgccaaccgg gagctccagt gggggatgaa taattctcgc cagcagatcg gcaaccacca
     2161 acagcaacgc acctgtcagg gccgaaacag gcagcagtct gcggtgacgt ccaccggtga
     2221 tgctacgcat catatgcggc accacgagac caataaagct aatcgggccg caggcggcca
     2281 cgccggtaga tgtcatggcg acagctagta acaaagccca gaatcgggta tggggcaccg
     2341 acacaccgag cgtggtggcg cgcgcatcgc cgagtgcaag gaggtcgaga tcgcggcaaa
     2401 aactcaggct cagcggcaga aataaaatca tcagcgggat ggcaatcttc acaaagctcc
     2461 agtcacggcc ccataagctg ccggtcagcc acagcagggc gttgttcaca tcctgcgggc
     2521 gcgagagcat cagataatcc gtcaggctgg cccagcatgc agaaagcgcc acgccggtga
     2581 gcgccagctt catcggctgg tgggtctttg ccagcatctt cagcaatatc aaccccgcca
     2641 tgccgcccgc aaaggccagc agcggcagca ccatcacggg cagtgacggc ataagaagta
     2701 gagcccccac agaggccagg ctggcggcat ggttaacacc gagaatatcc ggtgatgcca
     2761 gagggttgcg cacaatcccc tgtatcagca cgcccgccac ggcgagggct gcaccgacaa
     2821 acagtgccag cagcaagcgc ggcagtcggt actccatcaa tacataataa tgctcgcgtc
     2881 cggcctgcca gtcggtcagc agcgcgcgcc acggcacggg gatcactccc atatggagtg
     2941 ataacagcgc acagcccgcc agggcaaggg tgatgaaaat aaccagcgca attttcatcc
     3001 tcgcctcctc acaagccaga caaagcaagg gctgccaatc agcgccagca ctgcgcctgc
     3061 gggcagatct ccggggaagg ccagtgcgcg tgcgagtaca tctgccagca gcatcagcgt
     3121 ggcccccagc agcatgctca ctggcagtac gttgcgctga tcgaagcctg cccagaagcg
     3181 cgccagatgt ggcaccagca gaccgataaa cgccaccgga cctgccacgc tgacgcacgc
     3241 accaaccaga agcagcacta acatattgat gaccaaacgt agcctcgtca ggttcactcc
     3301 cagcgtatgg gcggtgctgt cgctgaggtt gagcaggttc agttgattcg ccagcagcaa
     3361 cacgacaggg actgcagtga ccaccaccgg caagagctgc cagacatcct gccagcgggc
     3421 gtgggacact ccgcctgcca gccagtaaaa gatgccgtaa gcatgatctt cggccagcag
     3481 cagggtgatg cgggtaaggc ccatacaaaa ggccgacagc gcgatacccg cgaggatcag
     3541 tttgtttctg tcatgggtat gacgaaatcc gcctcctgcg gtcatgacca gcagccagct
     3601 cacgccgccc ccgcatgccg cgatgaacga cagagaatag cctgcaatcg gcgtcggact
     3661 cagcgcgctg gtaagcgcca tagccagcgc cgcgccgctg ttaatgccga gcagtgaagg
     3721 agaggccatt gggttgtggg tcagggtttg cagcagcgtg cccgcgagcg ccaggcttgc
     3781 gccgatcaga acggcgacca ggcttcgtgg caaacgaagg ttttgcacca gcgcttctgg
     3841 tagcgttggc gtgtgtccag gcagcagggc gcgggttgca tctgctccgg aaacaggaat
     3901 ggccgagtag caaaacagac tcagccagaa aataataata agtgctgcaa cgggaagccc
     3961 ccacagcagc accgggtgtt ttatcgcggt catttcacaa cggtaagcgg ctgatggtgg
     4021 aagattttta ccgtgtcagc ggcaatacgc tctgcagcaa aaataccgcg catccgcgcc
     4081 caggtgttac tgtcgaccga agcaacctgc tgcttctgcg cggcggttaa catctgccag
     4141 agcggatctt gttgccagcg tttaacaatg ctctcttcgc gatagtgggc aaccagcagc
     4201 caggcaggat tgaccgccag cagttgctcc aggccgatgg acggcatgga cgcacccgcc
     4261 atcgcagcgg gaacgttcag ccccagagag gccagcacgc tgccggtcca ggtctcctga
     4321 gtatgcaggt tgaattgctg ttcgcgtgat gtgccaaagg ccacgcgtgt ccctttggga
     4381 agctggctgg cccactgcgc catcctctct ttatgttgtt ccaggcgtgc ctgcatctct
     4441 cgctttttac ccaccatttc gccgatgata gccgcagatt gcaaattttc agcgtaggtt
     4501 tcgttgcggg acttaagcag cagtaccggc gcgatttgct gcaaggcgat gtaaaccccc
     4561 gcatggcgac tgctgtcggc aatgatcagg tctggtttca gagcggcaat ggcttccagg
     4621 ctcggctgcg cgcgcgttcc gacggactgc cacggtttca ggtgcgcacg cacttcgggc
     4681 aggatgcgtt ttgcatcgtt atcgtcggca ataccgatcg ggctgacgtc cacggcggcc
     4741 agcgcatcgg cgaacgagag ttccagcacc acaatccgtt gtggcgtttt ttcgagtgta
     4801 aacgtgccgt gttcgtcctg aaccgtggcg gcaaaggcgt ggctgatcac cagcagcagg
     4861 cctgcaaaaa caaaacggat aaatgccaac ataatcacat tccagctaaa agcccggcaa
     4921 gccgggcgtt aacacatcag aacttcaacg acccctgcat atacagcgtg cgcggctggc
     4981 ctgcatagat gcctttgttg ttgtcgtcat aagagcggat gaagtagtcc tggtcgaaga
     5041 tgtttttcac accgaatgcc aggttcagat ctgccatctg cgggccaaag tcatacgcca
     5101 cgcgtgcgcc ccagagcatg aagccgggaa tgcggccggt actgccgtcg gcgctctctt
     5161 tcaccgtatt ggcgttatcc gcaaactggc tggactggaa atcgctgttc agattgaacg
     5221 tccagtttcc tggcttgtag tccacgccca gcgtgccttt atgtttcggg gagaatggta
     5281 ccagattgcc gtaggtgtcg cctttctcgc ggatttccgc gttcacatac gcatagctgg
     5341 cgtagatgga aacgttatca agcgttggcg ttagcgtacc cagatcgtaa cgtgcctgcg
     5401 tttccagccc ggtatggcgc gttttgccac gtgcagtgac ggtgtcgttg gtctggttgg
     5461 agtcgtactg attgttaaag ttaatcagga acagccccat ttccgccgtc agcgcgccgt
     5521 cgtcgtagcg ggtaccgagt tcccaggttc gcgctttttc cggttccaca ttgccgcttt
     5581 gcacagcctt gccaatctgg ctgtactgta cggtgccgaa cgagccttca gtgtttgcat
     5641 aaagattcca gctgtcagtc aggtgataga gcacgttcaa cgccggaagc ggtgcgttat
     5701 agctcacttc ttcgtgcgtg cctgtgatgg cgttgttctg gtatgactcg atatgttcga
     5761 aacgcatacc cggcgtgatg gtccagttgc cgatgtcgat tttgtcatcc agataccagg
     5821 cgtgcgcctc ggtgccggaa cgcgtatcgc ggtcgtaagg gcttgagccg gacggcaact
     5881 gcccgctgct ggtggcggtg tagtaacgca tttcatgcgt tgattcattc acatagcgat
     5941 agcccacgcc cacttcgtgc gcggaagggc cgatcataaa gatctggctg tagcgtggct
     6001 caataccgcg cacccagtag ttacgcggcg agagggtgat gcgtttgcct tgctccaggt
     6061 agccgctgcg cagggtttgg gtgtagaacc cctgaatgtt gaatttatgc tggctgtctg
     6121 gctggaactg gtagcccagg ctcgccagct tgcgacgacc ccagaagcgg tcatacgggc
     6181 gggtggattg ccagcgatcg gcgtcgtaat ccgcgcgaga caggccaccg ggcatgtcgg
     6241 cttcaccgtc gtaatattgc agcaggctgt tgaaggtgtg cacctcatcc ggcgcatatt
     6301 tgcttttcag catcaggtcg tcgatgcggg tggcgctgtg ctcgcgccag tcactgccgc
     6361 gcgtgccgga gtagagcagc gcggtgccaa aaccgttgtc cgctgtgccg cccaccatca
     6421 ggttgtgcgt ctctttcggg ttgttttgtg aagaggttgg gctgagctga ccttccacgc
     6481 ccgcctcgat accaaagtcc tgcggaatgg cacgggtaac aaagttcacc acgccgccca
     6541 cgctctgcgg tccgtaacgc accgcaccac caccgcgtac cacgtcaatg gcatccatgt
     6601 tgccgagcga aacgggagcc agtgaaagct gcggctgacc gtaaggggcg aaggggacgg
     6661 ggatgccgtc catcaggacg gtcgagcggc tggtgaggcg cgggttcagg ccccggatgc
     6721 caaagttcat cgccaggtcg tggctgccgg tgccgttgtt ttccggcgcg ctgacgccag
     6781 ggatgcggtt aagtacctca cgcatggtgg ttgcgccggt tttggcgaaa tcctcacggc
     6841 ggatcacgtc acgcgcgcca gcatgttcaa atacgtcgtt ttcacgcgca tcacccagcc
     6901 agtcgccgac cacggtcagg gcatcttctt ttggtgcggg cgcgggctcc agcgtccagc
     6961 tattatttcc cagcggtttt acctgcagtc cgctgccgtc cagcagttgt tgcaggccgc
     7021 tctcgacgtc gtaatcgccg tgcaggccgt tgctctgctt gccgcgcgtc aggctggcgt
     7081 caaccgagag ggtaaatccg ctgtgtgcgg catactgatt gagcgcttta tcgagcgatc
     7141 ccggtgcgat attaacctgt gcagcaaaag cggaaaacga gagaccggcc agcggcagca
     7201 ggctcaggcg aatggcgtta accaaaggtg ttgttttacg aaaaacgcgt aacggcgtca
     7261 taccttcccc atcatcattt ttgttgtgtt cagctatgag tcgaacgaga agggtaaaaa
     7321 ggacaatcga aataagaatt attttcctta cagtggtgaa atgtttatcc agtaccgcgt
     7381 aatagactga attttaacgg gaagcgtttg cgcgataacg ttcaggatcg catcggtatt
     7441 tttcagcggg aacgtcccgc tcaggcgcag cccggcaacg gcgggatcgc agcgcagcac
     7501 gccgttgcgg taacgggtta gcgtggctat cacctcaccc agtggtttat cgctgaagct
     7561 caggatgtcc ttcgtccagc ttgtactctc gtcatccagc ggtttcactg cgccaaactc
     7621 agaggcgctg aactgcaggc tttcaccagc gttcacgatg cgtttttgcg cgggggcact
     7681 ggcgagaagc acttccacag cgtgctgctg aacgtcaagc tgcgtgaaat tatcctgctg
     7741 gcggacggta aattctgtcc ctaaagcagt gagctggccc tgacgggtca ggacacggaa
     7801 ggggcgttgc agggcatctt tcgcggtagt aatggcgatt tcaccgtacc agagccggac
     7861 ggtgcgctga tgcgcatcaa aacgcacatc cgccgcgctt tgggtattca gcgtgagcag
     7921 ggagccatct tccagttgct gacggctgac tgtgcctttg gcggtgcggt aatctgcccg
     7981 cagaccttcg ccggtttccg actgccagag ctgccagcct ccgccagcgc cgagcaacag
     8041 cagtaatcct ttcatcacgt gacggcgggt gaggcgggta tcgtgcaacg cccggctggc
     8101 aacgtcgcca ggcacaccgc caagctggtt gcgcaggttt tcaacctgct gccaggccca
     8161 ctggttatcc tgatcctgtt cataccactg ttgccagcgc gcttcctgtt gtgggctgac
     8221 gcgctcgccg cttagcacgg cataccagtg ggaagctgaa cgcagcgcct gacggcggga
     8281 atcggttaac aaaggattca taacccatac tccagacgga acagcaggca gtgctcgacg
     8341 gctttcgcca cgtatttttt cacggagctg atggaaacac cgagtttgtg cgcaatctcg
     8401 ctgtatgtca gaccatccag ttgcgaaagc agaaacgctt cacgtgtttt gccgtttagc
     8461 ccgtccagca tgctgtcgag gagttgtagg gtctcgagtt ggctttcgcg ttcctcaggt
     8521 gaaggcgctc ccccctccgg cataagcgcc agcatctcca gatacgcttt ttccagcgcg
     8581 tttcggcgaa acaggtccac catcacgcgt ttggcgatag tgcagaggaa ggagcgagga
     8641 tcgcggatcg tcgagagcgt ttcgctgacc attacccgca aaaaagtgtc ctgggcaatg
     8701 tcatctgcat caaaagcaga ctggagtttg cgcgtcagcc agcttttcaa ccagccgtga
     8761 tgtgtgccat aaagcgactc gaacgttaag gaagctgtgg tagtggcgcg gtcagacatg
     8821 cggagtgcat caaaagttaa ttatcatgta gtcatattaa tatgagaatg gttatcatta
     8881 caattggaaa taaaattgtt tccaatagac atttttaaca tgttgttttt ctaagtgtta
     8941 taaggtaggt ataaaatggg atggagcctc tgcttctggc atgtgtcggt cagaatgact
     9001 catgatgtgg tctgctatta ttgacatcct cactgcccta aaggatgggg atttcggtaa
     9061 tgctgccaac ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggcttcca
     9121 tttccatcag atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca
     9181 ctgccggaca tcagcgctat ctctgctctc attgccgtaa aacatggcaa ctacagttca
     9241 cttacaccgc ctctcagccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg
     9301 tcggatgtcg cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact
     9361 taaaaaactc aggccgtagt cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt
     9421 ctgcgctgaa atggacgaac agtggggcta cgtcggtgct aaatcacgtc agcgctggct
     9481 gttttacgcg tatgacagga tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac
     9541 tctggccaca ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatagat
     9601 gacggatggc tggccgctgt atgaatcacg cctgaaggga aagctgcacg ttatcagcaa
     9661 gcgttacact cagcgcattg agcgacataa tctgaatctg agacaacatc tggcaaggct
     9721 gggacggaag ttactgtcgt tctcaaaatc ggtggagttg catgacaagg tcatcgggca
     9781 ttatctgaac ataaaacact atcagtaagt tggagtcatt acctcctccc gaactcaaag
     9841 ctatcataca gataattact ggttaaccct atatagggca ttttaaagtg ttgtgatctt
     9901 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgactcacaa
     9961 gacaaaacag tttcaaccag taaaaaaacg taaacaccat tcgcatgaat ttcgtagtta
    10021 agatctgaag cttgcagaat gcatcggtgt tactgccgga gtccgtgaac ttagtctgca
    10081 ttaatcacca cagctttaca tctgtcgcag taaacagcaa aatcagcaaa cttacttaat
    10141 tatatcgagt gcgattacaa tcgctggcga tggtacagtt ggtgtggcgg tctcagtccg
    10201 gaacaatttg aaaatcagaa tctcacttaa aacggcatcc attttacaaa agatggccgt
    10261 ctgtgtattt ataatgaata ccttctacat gtttagagtg cataatccac gacaaggcga
    10321 tatcctaaaa attagctagc aggcaacttt gatggcatag ataatgtttt tagtaaaaaa
    10381 gagttgactg aatataatac agcctcttcc tacataagaa ggaatgtgct cacaaaaaaa
    10441 accattagtc tgcttcctgt tcttacaata atcagtatta tgtattttat ttatatagca
    10501 aaatatgctt gttacacctg tcataattag gcatggtaaa attattgact gaaccctggg
    10561 ggagaaagat gcacagtgat tattataata tggtttttgg tgaaaaattg gccaatattt
    10621 tatatgaagc taactctcaa tttttttacg agagaaatgt catagaagag gcagtcaacg
    10681 ctttattctg tgag
//
LOCUS       DAZFRE010000140        10651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFRE010000140 DAZFRE010000000
VERSION     DAZFRE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.141"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHKUI_004485"
     CDS             complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHKUI_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO6600544.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIA"
     gene            222..716
                     /gene="napF"
                     /locus_tag="ACHKUI_004486"
     CDS             222..716
                     /gene="napF"
                     /locus_tag="ACHKUI_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6600545.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            706..969
                     /gene="napD"
                     /locus_tag="ACHKUI_004487"
     CDS             706..969
                     /gene="napD"
                     /locus_tag="ACHKUI_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6600546.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            966..3452
                     /gene="napA"
                     /locus_tag="ACHKUI_004488"
     CDS             966..3452
                     /gene="napA"
                     /locus_tag="ACHKUI_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO6600547.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3459..4154
                     /gene="napG"
                     /locus_tag="ACHKUI_004489"
     CDS             3459..4154
                     /gene="napG"
                     /locus_tag="ACHKUI_004489"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6600548.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDVRMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4141..5004
                     /gene="napH"
                     /locus_tag="ACHKUI_004490"
     CDS             4141..5004
                     /gene="napH"
                     /locus_tag="ACHKUI_004490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6600549.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTFIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5001..5450
                     /gene="napB"
                     /locus_tag="ACHKUI_004491"
     CDS             5001..5450
                     /gene="napB"
                     /locus_tag="ACHKUI_004491"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6600550.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5460..6062
                     /gene="napC"
                     /locus_tag="ACHKUI_004492"
     CDS             5460..6062
                     /gene="napC"
                     /locus_tag="ACHKUI_004492"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6600551.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHKUI_004493"
     CDS             6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHKUI_004493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6600552.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHKUI_004494"
     CDS             6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHKUI_004494"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6600553.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHKUI_004495"
     CDS             7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHKUI_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6600554.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHKUI_004496"
     CDS             8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHKUI_004496"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6600555.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHKUI_004497"
     CDS             8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHKUI_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6600556.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8815..>10651
                     /gene="ccmF"
                     /locus_tag="ACHKUI_004498"
     CDS             8815..>10651
                     /gene="ccmF"
                     /locus_tag="ACHKUI_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6600557.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2192 a         2718 c         3181 g         2560 t
ORIGIN      
        1 gctatataaa tatgtttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
       61 tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg catctttgac
      121 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
      181 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
      241 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
      301 ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt attaatgctt
      361 gtgaaaacaa cattctgcaa cgcggcgcgg gtggttatcc gagcgttaat ttcaaaaata
      421 acgagtgcag cttctgctac gcctgcgcgc aggcttgccc cgaatcacta ttttctccgc
      481 gccacaccag ggcctgggat ttgcagttca ctatcgggga agcctgcctg gcgtatcagt
      541 cagttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
      601 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
      661 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
      721 caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
      781 ctgaacgcct ttaccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
      841 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
      901 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
      961 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
     1021 cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
     1081 ttaaatggga taaagcgccg tgccgtttct gcggtacggg ttgcggcgtt ctggtcggaa
     1141 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
     1201 tgaactgcat taagggctat ttcctgccca aaatcatgta cggtaaagac cgtttgacgc
     1261 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
     1321 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
     1381 ggccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
     1441 cgtccaagct gtttaaagcg ggcttccgtt cgaacaacat cgacccgaac gcgcgtcact
     1501 gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag ccgatgggct
     1561 gctacgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgcaaac atggcggaga
     1621 tgcacccgat cctctggtcg cgcatcacta accgtcgtct ttctaaccag aacgtcaccg
     1681 tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
     1741 ttacgccgca atctgacctg gtgatcctga actatatcgc caactatatc attcaaaaca
     1801 atgcgataaa tcaggacttc ttcagcaaac acgttaacct gcgcaaaggg gcgacggaca
     1861 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccgggttctg
     1921 acgcctccga accaatgagc tttgaagatt acaaagcctt cgttgctgaa tatacgctgg
     1981 aaaaaaccgc cgaaatgacc ggcgtgccga aagaccagtt agaacaactg gcgcagctgt
     2041 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcataccc
     2101 gtggtgtatg ggccaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
     2161 agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg
     2221 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgactaac gagaaacatc
     2281 gtgatatttg cgagaagaag tggaatatcc cgagtggcac cattccggcg aaaatcggtc
     2341 tgcacgcggt ggcacaagac cgtgcgctga aagacggcaa actgaatgtt tactggacca
     2401 tgtgtaccaa taacatgcag gccggaccga acattaatga agagcgtatg ccgggctggc
     2461 gcgatccgcg caactttatc atcgtttccg atccgtatcc gacggtcagt gcgctggcag
     2521 ccgacttgat cctgccgacc gcaatgtggg tagaaaaaga gggggcttac ggtaacgccg
     2581 aacgccgtac tcaattctgg cgtcagcagg tacaggcacc gggcgaagcg aaatcggatc
     2641 tctggcagtt agttcagttc tcccgccgct tcaaaactga agaagtgtgg ccggaagagt
     2701 tgctggcgaa gaaacctgaa ctgcgtggca aaacgctgta tgaggttctg tatgccactc
     2761 cggaagtgag caaattcccg gtatccgaac tggcggaaga tcagctgaat gacgaatccc
     2821 gcgagttggg cttctatctg caaaaagggt tgttcgaaga gtacgcatgg tttggtcgcg
     2881 gtcacggtca cgatctcgcg ccgttcgatg attaccacaa agcacgcggc ctgcgctggc
     2941 cggtggtgaa cggtaaagaa acgcagtggc gttacagcga aggtaacgac ccgtacgtga
     3001 aagcgggcga aggctacaag ttctacggta aaccggatgg caaagcggtg atcttcgcgc
     3061 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
     3121 gacgcgtcct ggagcactgg cataccggca gtatgactcg ccgtgtaccg gaactgcacc
     3181 gcgccttccc tgaagcggtc ctgtttattc acccgctgga tgcgaaagcg cgcgatctgc
     3241 gtcgtggcga caaagtgaaa gtggtttctc gccgtggcga agtgatctcg attgtcgaaa
     3301 cgcgcggtcg taaccgtccg ccgcagggcc tggtgtacat gccgttcttc gacgccgcac
     3361 agctggttaa caaactgacg ctggacgcga ccgatccgct ctcgaaagag acggacttca
     3421 agaagtgcgc ggtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
     3481 aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg
     3541 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
     3601 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
     3661 ttcaggcttg cccttacgac accttaaaac tggcgaccct ggcctctggt ctgtcggcgg
     3721 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
     3781 ccaaagtgtg cccgagcggt gcgctggatc gtgagatcga atcgatcgac gacgtgcgga
     3841 tggggctggc ggtactggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
     3901 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
     3961 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
     4021 gctgcggtaa gtgcgaaaaa gtgtgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
     4081 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggctggctg gaggggaaca
     4141 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc
     4201 agtcaccgtt ggttagtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
     4261 agcggtccgt ggtttggtgt gtggatcttg cacggcaact acagcagtag cctgttattc
     4321 gacaccgttc cactgaccga tccgctgatg acactgcaaa gtcttgccag cggtcatctg
     4381 cctgccacgg tggcgttgac cggtgcggtg attatcacag tgctctacgc cctggcagga
     4441 aagcgattat tttgcagctg ggtctgtccg ctgaacccga ttaccgactt agcaaattgg
     4501 ttacgcagac gatttgacct caatcagtct gcgacgatcc ctcgccatat tcgctacgtg
     4561 ctgttggtgg tcattctggt gggatcggcc ttaactggca cgttcatctg ggaatggatc
     4621 aaccccgttt ctctgatggg gcgtagcctg gtaatgggct tcggcagcgg cgcgctgctt
     4681 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggcacatt
     4741 tgtccggttg gtgcactgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
     4801 accgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
     4861 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
     4921 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
     4981 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
     5041 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
     5101 gccagtcgcc ggaagtatcc gggacgcagg aaggggccat tcggatgcca aaagagcagg
     5161 atcggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
     5221 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agttatcgca
     5281 ccactggcgc gccgcgcatt agtcctactc actttatgga cagcgacggc aaagtgggcg
     5341 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
     5401 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
     5461 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
     5521 ccagccgtct ggcgctaggg acgctgctgt tgatcggttt tgtcggcggc atcgtcttct
     5581 ggggcggctt taacaccggg atggaaaaag ccaataccga agagttctgc attagctgcc
     5641 acgaaatgcg taacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
     5701 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccaaagatga
     5761 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
     5821 cgcagaaatt tgaagctcat cgcctgacga tggcacagaa tgagtggcgg cgcatgaagg
     5881 acaacaactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
     5941 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
     6001 attgccataa agggatagcg cacaagctgc ccgacatgcg tgaagtcgag ccaggttttt
     6061 aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgcgag cgggatgaac
     6121 gaaccttatt tagtggttta tcatttacgc tgaacgcagg agagtgggta caaatcaccg
     6181 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
     6241 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
     6301 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
     6361 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
     6421 ccgggcttgc cggattcgaa gatattcctg taaatcaact ttctgccggg caacaacgcc
     6481 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatactc gacgagcctt
     6541 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg
     6601 agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct gaaagtaaaa
     6661 ttcgccgcat ttcactgacg caaacggggg ccgcatgatg ttctggcgca ttttccgtct
     6721 tgagctgcgc gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct
     6781 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcgcgtat
     6841 tgcgccgggc attatctggg ttgctgcgct gctttcatcc ttgctggcac tcgagcgact
     6901 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
     6961 gcccgccgct gtgctggcga aggtgatggc gcactggatg gtaaccggtc tgccgttact
     7021 catcctttcg cccctggtag caatgctact gggaatggat gtttatggct ggcaagtgat
     7081 ggcgttaacg ctactgctgg gaacccctac gcttggcttt cttggtgcgc cgggcgtggc
     7141 gctgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt taccgctgac
     7201 tatcccatta ctcatctttg ccaccgccgc gatggacgcg gcttctatgc atttgcccgt
     7261 tgatgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
     7321 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
     7381 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
     7441 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc
     7501 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
     7561 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
     7621 gcggtggcag cgtttattgg cctggtctgg cagatgaaaa tggccaacct ggcggtggcg
     7681 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
     7741 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
     7801 ctgctgtttc tgtatgttgg tgtcattgcc ctgtggcacg ccttcgacga ccgtcgtctg
     7861 gctggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
     7921 tactccgttg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
     7981 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc
     8041 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
     8101 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
     8161 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
     8221 ttccgctggt ggttttggtc gtgcactcgg tgatgcaaca tcgcgcaatt ctgcgtggcg
     8281 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
     8341 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgcc gtgctggcgg ggctggcgct
     8401 gactatcggt ctggtgctat atgcgctgcg ctcgaatatt gatctctttt atacgccggg
     8461 ggagattctc tacggcaagc gtgaaactca gcagatgccg gaagtcggtc agcgtctgcg
     8521 cgttggcggg atggtgatgc cggggagtgt gcagcgcgat cccaattcgc taaaagtaac
     8581 tttcaccatt tacgacgctg aaggttcagt agatgtctct tacgaaggca ttttgccgga
     8641 tctgttccgt gaagggcagg gcgttgtggt gcagggcgaa ctggaaaaag gcaatcatat
     8701 cctcgcgaaa gaagtgctgg cgaaacacga tgaaaactac acgccgccag aagttgagaa
     8761 agcgatggaa gctaaccacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
     8821 ccagaaattg gtaacgggct gctgtgtctg gcgctaggaa ttgcgctgct gctgtccgtg
     8881 tatccgctat ggggcgtggc gcgcggggac gcgcgcatga tggcgtcttc ccgcttgttt
     8941 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tgctggtcaa tgctttcgtg
     9001 gtcaacgact ttaccgtcac ctatgttgcc agcaactcca atacccagct tccggtgtgg
     9061 tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tcctgctgtg ggtgctactg
     9121 atgagcggct ggacctttgc ggtagcgatt tttagtcagc gtattccgct ggatattgtt
     9181 gcccgcgtac tggcgataat ggggatggtc agtgtcggct ttttgctgtt cattctcttt
     9241 acctctaacc cgttctcacg cacgctgccg aatttcccga ttgaagggcg cgatcttaac
     9301 ccactgttgc aggatccggg gctaattttc cacccgcctc tgctctatat ggggtacgtc
     9361 ggtttctcgg tggcgtttgc ttttgccatt gcatctttgt tgagtgggcg tctggacagc
     9421 acttatgcgc gttttactcg tccgtggacg ctggctgcgt ggatcttcct gacgctcggc
     9481 attgtgctcg gttccgcatg ggcctattac gaactcggct ggggcggctg gtggttctgg
     9541 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
     9601 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
     9661 gccttctcgt tgtgtctgct ggggactttc ctggtgcgtt ccggcgtgct ggtatcggta
     9721 cacgcgtttg cctctgatcc ggcacgcggt atgtttatcc tcgcctttat ggtactggtg
     9781 attggtggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
     9841 aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct ggtcgccgcg
     9901 atgctggtgg tattactggg gacgctgctg ccgttagtgc acaagcaact gggactgggc
     9961 agcatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    10021 gcgctgctgc ttggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    10081 cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtcgctgct gctgccgtgg
    10141 ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    10201 gcggtgctgg caattgcgga agctgcgctg cgtatttcac gcggtacgaa aaccaccttc
    10261 agttattggg gaatggtggc ggctcacctg gggctggcag tgacaattgt tggtattgcc
    10321 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagtgtcgat
    10381 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    10441 ggcggtgtgg cgactatcgg cgtaacgcgc gatggcaagc cggaaacggt gctgtatgcg
    10501 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcgat tgacggcggc
    10561 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggccgtg
    10621 cgtctttact acaaaccgtt tgttcgctgg a
//
LOCUS       DAZFRE010000141        10586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFRE010000141 DAZFRE010000000
VERSION     DAZFRE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.142"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(58..3960)
                     /gene="hrpA"
                     /locus_tag="ACHKUI_004499"
     CDS             complement(58..3960)
                     /gene="hrpA"
                     /locus_tag="ACHKUI_004499"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6600558.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4161..4766
                     /gene="azoR"
                     /locus_tag="ACHKUI_004500"
     CDS             4161..4766
                     /gene="azoR"
                     /locus_tag="ACHKUI_004500"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6600559.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(4820..6112)
                     /gene="ynbD"
                     /locus_tag="ACHKUI_004501"
     CDS             complement(4820..6112)
                     /gene="ynbD"
                     /locus_tag="ACHKUI_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6600560.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFICSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWIVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(6126..7883)
                     /gene="ynbC"
                     /locus_tag="ACHKUI_004502"
     CDS             complement(6126..7883)
                     /gene="ynbC"
                     /locus_tag="ACHKUI_004502"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6600561.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLLPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVAHLHAKGLAVRVVDIAAGHGRYVLDALANEPAISEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDTGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(7899..8795)
                     /gene="ynbB"
                     /locus_tag="ACHKUI_004503"
     CDS             complement(7899..8795)
                     /gene="ynbB"
                     /locus_tag="ACHKUI_004503"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6600562.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITTMI
                     ASLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8795..9400)
                     /gene="ynbA"
                     /locus_tag="ACHKUI_004504"
     CDS             complement(8795..9400)
                     /gene="ynbA"
                     /locus_tag="ACHKUI_004504"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6600563.1"
                     /translation="MTLYQIKPLFQPLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPVSNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(9699..10559)
                     /gene="pdxI"
                     /locus_tag="ACHKUI_004505"
     CDS             complement(9699..10559)
                     /gene="pdxI"
                     /locus_tag="ACHKUI_004505"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6600564.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPTEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLNATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
BASE COUNT         2752 a         2864 c         2483 g         2487 t
ORIGIN      
        1 aaaaaacccg gcgctaaatg cttaccgggt tttagattta tcaggcaaat agcagtgtta
       61 accgctaatc tgctccatcg cctgcaaaat acgcttatcc gaaatcggat aaggcgtacc
      121 aagttgttga gcgaagtaac taacgcgcag ctcttctatc atccagcgga cctctttcac
      181 gtcttcatcc tcacgacgtg caggcggcag tttgttgatc cattgctgcc atgcctgctg
      241 gacgttttcg actttcagca tctgagcacg gtcacgatgc ggatcaatcg ccagtttttc
      301 cagtcgtttt tcaatcgcct gcaaataccg taacgtgtcg cccaaccgtt taaaaccgtt
      361 gccagtgaca aaaccgcgat acaccagccc gcccatctgc gctttaatgt cagaaagccc
      421 cagcgccatg gtcatatcca cccgcccttt cagacgtttg ttgatattga acaccgcagt
      481 gaggatttgc tcgacctgct tcgcaatatc caccaccgtg tcgttcagtt cagcgcggac
      541 tttttcatgc agcgcagcaa agccttcttc cgtccagacc gggccaccat tggcgtcgat
      601 cagttgatcc acaccgcagg agatacagtc atcgatcagc tccagcactt tgccatacgg
      661 attaaagtac agccccagct tggctttgtt tggcagcttt tcgtgcaagt acttgatcgg
      721 cgatggaata tttagcagca gtaaacggcg aagaccgttc cacattgcct gcttctgttc
      781 cagcgggtta tcaaacagtt tgatcgctac gctgtcacgc tcatccacca gcgccggcca
      841 cgccttcact ttgtagttgc cgcgcttctg ctcgtaactt tccggaagtt gaccaaaact
      901 ccagatatgc agcccgctct gctcgatacc gtcatccgcc acggcagaca gcgtttcctg
      961 cactttgcct ttcagggcgt ctttcagatc ctgtaatgag cgcccttctt ttagcttctt
     1021 gtttttgtca tccaccacgc ggaaagtgat tttcaggtga tcgggcacct gatcccagtg
     1081 ccagtcttcg cggtcaacgg tgacgccagt catccgccgt aactctcgtt caaggctgtc
     1141 gagcaacggc aattccagcg gtgtgacgcg gcctaaaaac gcttcggcgt agtttggcgc
     1201 gggcacaaaa ttacggcgta ccggcttcgg cagcgattta atcagggcga tgatcagctc
     1261 acgacgcaga ccggggatct gccactcaaa cccactctcc tcaacctggt taagtagcgg
     1321 cagcgggata tgcacggtca caccgtcagc atccgcgccg ggctcaaact gatagctcaa
     1381 acgcagtttg agattgccct gatgccagaa gttcgggtaa tccagtttgc tgattttttc
     1441 cgccccttct ttgatcaaca tgctcttttc aaagttgagc aaatcaggcg tttcgcggct
     1501 gacttttttc caccaactgt cgaaatggcg tgcggaaatc acatcgtggc tgatgcgctg
     1561 gtcgtagaac tcaaacaacg tttcgtcatc caccagaata tcgcggcgac gagttttgtg
     1621 ttccagttct tccacttcgg cccgtagttt caggttttca cggaagaatg cgtgacgcgt
     1681 ctgccagtca ccttccacca gcgcgtggcg aataaagagt tcacgacata acgccggatc
     1741 gatctggctg tagttgacct tacgcgcagc aacaatcggc aacccgtaaa cggtgacttt
     1801 ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtgc ggttcgctgt aggtgcgttt
     1861 gattaaatgc tgagcgacag gttccaccca ttctggatcg atgcgtgccg caatgcgccc
     1921 ccacaggcgg ctggtttcta ccagttccgc caccattacc catttcggcg gctttttgaa
     1981 taaaccagaa ccggggaaga tggagaaacg cgcgttacgt gcgccggtat attcttgttt
     2041 atcggcatct ttcatgccga tatgcgaaag caaaccggtc agcaacgcaa tgtgaatttc
     2101 gcgataatcc gccggttcgc tgttaaccgg aatgccaagt tctttcacca cctggcgcaa
     2161 ctgggtgtag atatcctgcc attcgcgcac gcgcagatag ttgagataat cagtacgaca
     2221 caggcgacgg aaggcgttag aagaaagcgc cttttgctgc tcgccaagat aattccacag
     2281 atttacaaac gcgaggaagt cagactcttt gtcgtggaag cgacgatgtt tttcgtccga
     2341 tgcctgctgc ttgtccattg gccgctcacg cgggtcctga atagagagcg cggacgtgat
     2401 aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc agcaccatac gcgccagacg
     2461 tgggtcgaca ggcaactgcg agagctggcg accgagcggc gtcagtttat aggcgctggc
     2521 ctgttcatca gtggtgatcg cgcccagctc ttcgagcaga cgcacgccat cctggatatt
     2581 gcgtttatcc ggtgcttcga caaacgggaa tgcggcgata tcgcccaagc ccagtgcggt
     2641 catctgcaaa ataaccgagg ccaggttagt acgcagaatt tctgggtcgg taaactccgg
     2701 gcgcgagagg aaatcgtctt cggaatagag acgaatacag atcccttctg acacacggcc
     2761 gcagcggcct ttacgctgat tagctgaagc ctgcgaaatt ggctcaatcg gcaaacgctg
     2821 cactttggtg cgatagctgt agcggctgat acgcgccgta cctgggtcga taacgtattt
     2881 aatacccggt acggtcagcg aggtttccgc gacgttggtc gccagtacaa tgcgtcgccc
     2941 gctgtgcgac tggaacacgc ggttctgttc gctgttggaa agccgtgcat aaagcggcaa
     3001 gatttcggta tggcgtaagt tcagcttgtt cagcgcatcg gcggtatcgc gaatttctcg
     3061 ctcgccgctc ataaagatca gaatgtcgcc agggctttcc tggctcagtt catctacggc
     3121 gtcaaaaatc gcctgcaact ggtcgcgctc ggtgtcatcg gcttcttcaa caatcgggcg
     3181 atagcgcact tccaccggat aggtccgacc ggagacttcg ataatcggcg cattattaaa
     3241 gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg ataataattt ttaggtcagg
     3301 acgccgcggc agcaactctt tcaaatagcc gagaagaaaa tcgatattca ggctgcgttc
     3361 gtgcgcttcg tcaataatga tggtgtcgta ctgcatcagc aggcggtctt gctggatctc
     3421 tgccagcagg atgccgtcgg tcatcagctt gaccatcgtg ttatcactaa cgtgatcgct
     3481 gaaacgcact ttgtaaccga tgcaaccgcc cggctccgtt tttagctctt ccgcaatacg
     3541 gttcgccacc gttcgcgccg ccagccgacg cggctgggta tggccgatca gccctttaat
     3601 cccgcgaccc agctccatac agattttcgg tagctgagtc gttttaccgg aacccgtttc
     3661 cccggcgacg atcaccacct ggtggtcacg aatcgcttcg agaatgtcct gctttttctg
     3721 actaaccggc aaattgtccg ggtaagtaat ctccggtcgt gccgcttcac gcagcaggac
     3781 tttccctgcc gcatggtcaa tctctttcgc catctcctgg aaaatggcct gttgtgcatc
     3841 aggattttta accttcttca cgccgtgcag acggcgagaa aaacgcagtc tgtcacgcag
     3901 catcatcgaa tccagccgct gctgcaaggc agtaaaggtc aatttttgtt gttctgtcat
     3961 aacgttagtg ggcagtagtc tgcctgatta attttctttt aaatgataga tatagagtac
     4021 cacatcgcgg ctttctatgc gttgttcaat aaattcgaac ataggcttcg atttattgcg
     4081 ctatctctgt ggcagaattg tgaataaagt gtcaacaagc aacggggcat tgcccattca
     4141 aatatctata aggaaacacc atgagcaagg tattagttct taaatccagc atcctggcag
     4201 ggtactctca gtctaatcag ttgtccgatt attttgttga acaatggcgc gaaaagcact
     4261 ccgctgatga aatcaccgtg cgtgacctgg ctgcaaatcc gattccggtc ctggatggcg
     4321 aactggttgg tgcactgcgt ccgagcgatg cgccgctgac tccgcgtcag caggaagctc
     4381 tggcgctttc cgatgagctg attgccgagc tgaaagccca cgacgttatc gttattgcgg
     4441 caccgatgta taacttcaat atctcgaccc agttgaaaaa ctattttgac ctggttgcac
     4501 gtgcaggcgt tactttccgc tataccgaga agggtccgga aggcctggtc accggtaaaa
     4561 aagccatcgt tatcaccagc cgcggtggga tccataaaga tggcccaacg gatctggtga
     4621 cgccgtatct gtccacattc ctgggcttta tcggcattac cgatgtgaaa tttgtcttcg
     4681 ccgaagggat cgcgtacggc ccggaaatgg cagcgaaagc acagtctgac gcgaaagcag
     4741 ccatcgacag tattgttgct gaataaccgg acacgatctt gatgtggact gtagatattc
     4801 agtccacatc tcaatccgct tacctgtttt cccataattt cagcatcgct ttgtgctctt
     4861 ccttaagcac aatccgcgag cgtctggctc gaatataact aatcgcttca tcaacggttt
     4921 tacaatgtcc gtaacataac agccatgccg ccaccaccag cgcactgcgc gataatccca
     4981 acgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg
     5041 cctgtcggag ctccccctct tccggaacca ccagatccaa catcggtaca caaaaataga
     5101 gtcgatcttt tgtcgctcgt ccgcgaggga attcaaaggt gacgtccaga accgcattct
     5161 gtgccggaat atgtcgtgga aacgccccta aataaacacc agcagtaatt ttgctcaccg
     5221 gctccaggcg aagacaaaac caacgcatag acagccacat cccgatacgc cagggcaatg
     5281 tcagccagta aacggcgggc gggagtttcc cctgactatc tttccctgtt gttatcgcgc
     5341 caagcccacc gtacccacgg ccaatgatga atagcgataa tactggccaa cataaccaga
     5401 ctgaccacca taactgaagc attatcatta gctccatcaa cacaatgcac gagcacgcgc
     5461 ctacgacata tggcagtgct attttgattc gacgttgatc aggtttctga taattccaac
     5521 gacggtcgac gggcactatc cagtcaatta acatacctac cgccagcccc gtgatgacat
     5581 caataaaatg atgctgccag gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc
     5641 cgcagacttt acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc
     5701 agagaataat atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg
     5761 aaaatagcca tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcggat
     5821 agagaaaaaa accgcagcag gccattaccg ttgccagaat aagccggtga accagtcggc
     5881 gctgttcgaa tgtcgagcta caaatgaaca gcgaaaatcc atataaaaga tccagactcc
     5941 agtaaggaac aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac
     6001 tggggatatc atggttgtta agatcctgag tcgcggtgaa ctgattaaga aatccatatg
     6061 taaagaagaa aaacggggcc agcaacaata accagccagc tccttgtagc aatacgttcc
     6121 ggcgttcagt tatcacgacg caccgccatc gaaaccgtaa aaatgcccca ctcatcaatc
     6181 cgttgtgtgc atttatcaaa tccggtatca tgcacgagcg aatccatctc cccttgagaa
     6241 cgcacgcgca ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc
     6301 tccagttgtg ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg atcgatggca
     6361 ttggcaagac ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatacagg
     6421 ccagagacaa tcgccagcgt aggccgcgga gttaacgtgc tgagttctgc cggattaaac
     6481 gcatcgccct gttcaaaacg cgcccgccca gacattcccc gttgggcaat catctcttgc
     6541 ccttgtgcaa catttacctc actgtaatca cgtaacaaaa tttcgcttat ggcgggctcg
     6601 tttgccagcg catccagtac atagcgccca tgccctgcgg caatgtcaac cacgcggacg
     6661 gctaaacctt tggcgtggag atgggcaacg gcttgtttaa tcagcatttg taaatgggtt
     6721 ttgcgctggc gaataccgcg ccagccaaca ctgttcaggt agattttgtc gataaagcgc
     6781 ccgaatgcgt tactgccttg cggttgattt tgatagacat aatccagcga actgcctgaa
     6841 tcaaagccgg tactcattcc gagatgcagg cccgcagagt gcgccccgaa cagtttcatc
     6901 gcctttcgca taaagcgata cgccaaatca accggcgata acggcacggg tccaccagaa
     6961 aggagccgcc atcgatccgc tgatggtcca gtgcggtctt catgctgata atcaaatttt
     7021 tgtgacttgt tagcatataa acggctgata aagctttgca ttttttcaaa tgcctgcgcc
     7081 ctgttctctt cacccaacgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga
     7141 ggactacgta aacgctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatcg
     7201 cctgatatca gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca
     7261 gacgttttat agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca
     7321 ttattgaaac tcgccacccg ttgccgatcg tgggtcagat aacgcccttt cacataggaa
     7381 ttaataaaaa acagaccacg cagacgatgc cataacgcca gcccaggacg tgccagcggc
     7441 acatacaatt taaccttaaa ggccggagat gccagcacta gtccgcgaat agcaggcgca
     7501 taatcatgaa cccatgtggc aaccatcact gcgccgacgc tttgcgcgat cacaaccacc
     7561 tcttccagtc cgacctggct gtcgctggca gcaaaacgga caaattcatc aacatcctgc
     7621 actgaacgcg caagagacgg actataacca cgcggccccg aagtttgtcc atgccctcgg
     7681 gcatcccatg cataaaaagc agtatctggc atcgccagtt catcaacgat atgttgtaga
     7741 cgaccagaat gttcatgacc gcgatgaaat aagacgatga cctttttcgc ccctggcagt
     7801 aaagtgggcc agtgccgata aaacaacgct gtattatcac tggtggtaaa aaaatgctcc
     7861 ccagggatgc gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata
     7921 aaataaaaaa ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca
     7981 tgtcctggta atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc
     8041 actacatcgc cgcaaaaacc actaataccg attaacaaac ccgctaataa tgcctgtaat
     8101 gtatctagcg gtgtaagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg
     8161 ccgcccatca gaccttccaa tgtttttccc gggctgactt tagggaccac ttttcttctg
     8221 ccgcaggatt ttccccataa atactgtgca atatcgtttg actcggttaa tgccaaaaga
     8281 aataacacca gtaacgcccc gccctggata tttgtggttg gcaacattaa taaccaggcg
     8341 gcatgactca aggcgaaaac ggtcattatc cagccacaaa aaatggcgct cacggtatgc
     8401 agaaaaccgg aggggtcacc cactaaaact cgccaggtgg ctaatatcag aaacccagcc
     8461 aaaggtataa ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga
     8521 ggaatacccc aatataacca acgcggaaaa tgtacagata taagcgtaca atattctttc
     8581 aatgccagaa aacttatcag cgcaaaaaac gtcaacgtca tccagcgtgg acctgaaatc
     8641 accagtgaaa aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacctcaccc
     8701 ccttttctct ccggtaaccg ccataccaga aaacggttta tcagcgttgc taaaattaac
     8761 aacgcgaaaa gtgttgccag agatttttcc agcattatct ttcagcactc ataaaaagca
     8821 cactccgaca acgattaatc gcagtccaga gaagcagaat tgaagcaatg ctccataaaa
     8881 gattattcca ttgcatccac tgtggataaa tggcaacggc cagcccccac aatccaaata
     8941 ttaacgcgcg atcgcttttg ccaaatggac cggcataact gcgaataccg ttaatcgtct
     9001 gcgcgagtaa accgcaaaac tcagtcaata tggtgcaaaa taacatgagt attacgagtg
     9061 atgcgttact taccggtaaa aataaaaacg gtaaataaag cgcaatatcg gaaataacat
     9121 cgccagtttc attcaaaatc gccccaagac gtgtttgctg attgcactca cgcgccatca
     9181 tgccatccag cgcattgagt gccatacgga caaaaagcac gatgggcaat agcaaaaaga
     9241 ggatgggttg tgcgaccaac atcaacagca atcctgtaat aagagaaagc gccagtgcag
     9301 caagagtgat gtgattcgct gtaacatggt gcttataaag ccaaaacatc gtcggtctta
     9361 acagcggctg aaagagcggt tttatctggt agagcgtcat ttatcattcc gtgatattca
     9421 gtttgctcta tggtgcatca attccgacgg cggagatatc acaacgcgca gcatcaacgt
     9481 aaattcggaa tatatcaaga tgtggtggat gaatgcccta cacatctgtt tgattaccgt
     9541 ctggatatca gtgagatacc atctatcact gtacgaatca atgaatccat cgccttttcc
     9601 ctgattatcc agccgcaaat cctggcctta aacatatgca ctccatgcca ttaaaaaaga
     9661 aacaaaaggg aaatgcatct ttcccttttg tatattcgtt attctcgcga aataccatcc
     9721 aacgtagaca acactttctc agaaagatgc aatttttcag cagccatatt ctcccgtaaa
     9781 tgcgcaacag aagatgttcc aggaatcagc aaaatattcg gtgaacgctg taacagccac
     9841 gccagcgcca cctgcattgg tgtcgcatta aggctcgcag caacatcgga aagcgtggag
     9901 gattgcagcg gcgtaaagcc cccgagcggg aagaacggca cgtaggcaat gccatcacga
     9961 gccaaagcat caatcattgc gtcatcagca cggtgggcaa tgttgtattc gttctgcacg
    10021 cagacgattt cggcaatctt tcgcgcctct gcaacctgcg tcggtgtgac gttactcagg
    10081 ccaatatgtt ttaccaggcc ttgttgttgc atctccgcca gcactgtcag gctggcctca
    10141 atcgatcctt ccgtcgggcc atgaccatcc cccatcataa cgcgcaggtt aaccacatcc
    10201 agtacgtcca ggccgagatt acgtagatta tcgtgtaccg ctttttgcag ctctgccgga
    10261 gaaaatgcgg gtaaccagga agcgtcctct ccacgccgcg caccaatttt agtgacaatt
    10321 gtcaggtcgt cagagtacgg ataaagcgct tcgcggataa tctgattggt gacgtgcgga
    10381 ccataaaagt cgctggtatc aatatgattg acgcccaatg ccagcgcctc gcgcagcacg
    10441 gttatagcga cgtggcgatc tctgggcggg ccaaaaaccc caggaccagc cagttgcatc
    10501 gcgccataac caagacggtt aacggatttt gtaccgagag taaatgtgtt gctgctcatc
    10561 gtaccctgcc ctgtaataaa acggat
//
LOCUS       DAZFRE010000142        10170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFRE010000142 DAZFRE010000000
VERSION     DAZFRE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.143"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            206..709
                     /gene="ftnB"
                     /locus_tag="ACHKUI_004506"
     CDS             206..709
                     /gene="ftnB"
                     /locus_tag="ACHKUI_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6600565.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(788..1039)
                     /gene="yecJ"
                     /locus_tag="ACHKUI_004507"
     CDS             complement(788..1039)
                     /gene="yecJ"
                     /locus_tag="ACHKUI_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6600566.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1154..1240)
                     /gene="azuC"
                     /locus_tag="ACHKUI_004508"
     CDS             complement(1154..1240)
                     /gene="azuC"
                     /locus_tag="ACHKUI_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6600567.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1504..1827
                     /gene="yecR"
                     /locus_tag="ACHKUI_004509"
     CDS             1504..1827
                     /gene="yecR"
                     /locus_tag="ACHKUI_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6600568.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1999..2496
                     /gene="ftnA"
                     /locus_tag="ACHKUI_004510"
     CDS             1999..2496
                     /gene="ftnA"
                     /locus_tag="ACHKUI_004510"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6600569.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2534..2773)
                     /gene="yecH"
                     /locus_tag="ACHKUI_004511"
     CDS             complement(2534..2773)
                     /gene="yecH"
                     /locus_tag="ACHKUI_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6600570.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2964..4175
                     /gene="tyrP"
                     /locus_tag="ACHKUI_004512"
     CDS             2964..4175
                     /gene="tyrP"
                     /locus_tag="ACHKUI_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6600571.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4237..4902)
                     /gene="yecA"
                     /locus_tag="ACHKUI_004513"
     CDS             complement(4237..4902)
                     /gene="yecA"
                     /locus_tag="ACHKUI_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6600572.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5098..5184)
                     /locus_tag="ACHKUI_004514"
     tRNA            complement(5098..5184)
                     /locus_tag="ACHKUI_004514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5148..5150),aa:Leu,seq:taa)
     gene            complement(5197..5270)
                     /locus_tag="ACHKUI_004515"
     tRNA            complement(5197..5270)
                     /locus_tag="ACHKUI_004515"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5236..5238),aa:Cys,seq:gca)
     gene            complement(5324..5399)
                     /locus_tag="ACHKUI_004516"
     tRNA            complement(5324..5399)
                     /locus_tag="ACHKUI_004516"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5364..5366),aa:Gly,seq:gcc)
     gene            complement(5551..6099)
                     /gene="pgsA"
                     /locus_tag="ACHKUI_004517"
     CDS             complement(5551..6099)
                     /gene="pgsA"
                     /locus_tag="ACHKUI_004517"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6600573.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAL
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6156..7988)
                     /gene="uvrC"
                     /locus_tag="ACHKUI_004518"
     CDS             complement(6156..7988)
                     /gene="uvrC"
                     /locus_tag="ACHKUI_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6600574.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7985..8641)
                     /gene="uvrY"
                     /locus_tag="ACHKUI_004519"
     CDS             complement(7985..8641)
                     /gene="uvrY"
                     /locus_tag="ACHKUI_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6600575.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8937..9113
                     /gene="yecU"
                     /locus_tag="ACHKUI_004520"
     CDS             8937..9113
                     /gene="yecU"
                     /locus_tag="ACHKUI_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6600576.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9100..9324
                     /gene="yecF"
                     /locus_tag="ACHKUI_004521"
     CDS             9100..9324
                     /gene="yecF"
                     /locus_tag="ACHKUI_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6600577.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9392..10114)
                     /gene="sdiA"
                     /locus_tag="ACHKUI_004522"
     CDS             complement(9392..10114)
                     /gene="sdiA"
                     /locus_tag="ACHKUI_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6600578.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2584 a         2545 c         2318 g         2723 t
ORIGIN      
        1 atcaatgaaa tgtaaaaata tataaacttg atgatttaag cattttctta tacccgttca
       61 gacgttattc ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg
      121 cagaaaggaa ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc
      181 acttctggat ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc
      241 tcaaatgaac cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc
      301 tgaacaaagt ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac
      361 ccaaatgatg cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc
      421 cattgatgtt cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga
      481 agaatacgag caacgttcaa gcacgctggc gcagttagcc gatgaagcga aagaactgaa
      541 tgatgattca accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggcct
      601 gttactgcaa accattcttg atgaagtgcg cagtgcgaaa cttgctggta tgtgtcctgt
      661 gcagaccgac caacatgttc tgaatgtcgt gtcgcatcag ctgcattgat catacatcgg
      721 cgctaatgca ttgcgccgat gaagtttttg agaaaccgct gcctcatttt tttgaagcag
      781 cggtttttta atgggattcg ccctgtgggg taaacttgag ttcaatcaac gcgatggctt
      841 tttggattgc ccgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat
      901 tcttcagctg gctggacatt ttttcgcgaa cttcaacggg cgcgatcaca tcgagaacat
      961 ccagaatttg tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg
     1021 cattcagcgg ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg
     1081 gcttcacatc ttcagcgata gtcacatcca ccgtcatcag aacacaaaaa aacctgccgg
     1141 agcaggtttt ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat
     1201 agttattgaa catacttttc aggatcttgc gcagtttcat cgcggtactc cgaccatttg
     1261 ttatacaggt gttattgtct ttgcgtttat aatatgacaa ccatcacaaa aatcaatctt
     1321 tatgtgatgt aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaagca
     1381 gcccccccaa tttctgacac atatagatgg caagaaatag cgcctgccag gcgtcttttc
     1441 cggccattgt cgcagcgctg taacgcgtaa aatactgctt tcttttactc ttctggctgg
     1501 accatgagac ttctgattct gactctttcc ttaataacgc tgacaggatg tacggttact
     1561 cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgttcggtt ggtttatgat
     1621 caggcttttt tgcagcatgc ccgtaccgat cgttatgtca gtcgcgggat cgccgatcgc
     1681 gcatgccagc aggcaggcta tacccacgct atcccctttg gtcagccagt aggcaactgt
     1741 agcctctttg cgggttcctt atgtttgaat acggaattca ctttgtcata tcagtgccat
     1801 cattccgcct tccctgtctt tctctaaacg ttttaatcag cgagggggat cttcgctgat
     1861 taaataaata gcggtaatgc gttttattcc cattcgtatt tttaataaat gatgtttata
     1921 ttttaccttt tgcaaataat aaaataacaa attatagtga cgctacagca acaaatataa
     1981 cctttgtgga gcactatcat gctgaaacca gaaatgattg aaaaacttaa tgagcagatg
     2041 aacctggaac tgtactcttc actgctttat cagcaaatga gcgcctggtg cagctatcat
     2101 accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc aggaagagat gacgcatatg
     2161 cagcgtctgt ttgattacct gactgatacc ggcaacttac cgcgtattaa taccgttgaa
     2221 tctccgtttg ctgaatattc ctcacttgat gaattattcc aggaaaccta taaacacgaa
     2281 caattaatca cgcagaaaat taacgaactg gctcatgctg caatgaccaa tcaggactac
     2341 ccaacattta atttcctgca atggtatgtt tctgagcagc atgaagaaga gaaactgttc
     2401 aaatcgatta ttgataaatt aagcctggca ggcaaaagtg gcgaaggtct gtattttatc
     2461 gacaaagaac tctctaccct cgacacacaa aactaatgct ctccggcggc aggttttctg
     2521 ccgccgtttc aaatcagtga cggcaaatct tactctgatc ggttgaaaaa ccttcttctg
     2581 aaggtataaa tttgcctttt gctgccagaa acgccaccaa ctctcccgca gtcatccctt
     2641 ctgccgagca ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt gctgcttcca
     2701 gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact tcatgaccgt
     2761 gaatagagtc catcgttcct cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag
     2821 actttgcgcc agcgcagaac tgaacgcttt ttgttgtaca tttatattta catcatatgt
     2881 aacgtcggtt tgacgaatca gccgttatgc cttaacctgc gccgcagata tcactcataa
     2941 agatcgtcag gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg
     3001 cgggaaccac aattggcgca ggcatgctgg caatgccgct ggctgcggca ggtgttggtt
     3061 ttagcgtcac gttaatcttg ttgattgggc tttgggcgtt gatgtgctac acggcgctat
     3121 tactgctgga ggtgtaccag catgtcccgg cagataccgg tctgggcacg ctggcaaaac
     3181 gctatctggg acgctacggt cagtggctga cgggcttcag tatgatgttt ttaatgtatg
     3241 ctctgactgc ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact
     3301 ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg
     3361 gcgtggtttg tgtcggaact tcgctggtcg atttatttaa ccgttttctg ttcagcgcca
     3421 aaattatttt tctggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc
     3481 ttttaaccct gccgttgcaa cagggactgg cactgtctgc aatcccggtg atttttacgt
     3541 cgtttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta
     3601 agctacgctg ggtgtttata atcggtagtg cgatccccct ggtggcatat attttctggc
     3661 agctggcgac ccttggcagc attgattcaa caacctttat gggattgctg gctaatcatg
     3721 ccggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc
     3781 tggcagtgca tttatttgct gatttagccc tcgccacgtc cttcctcggc gtagcgttag
     3841 gcttatttga ttatctggca gatttgtttc agcgttcaaa taccgttggt ggacgattgc
     3901 aaactggtgc aattaccttt ctgccgccac tggcgtttgc actgttttat ccacgaggat
     3961 ttgtgatggc gctgggttac gccggtgtgg cactggcggt actggcattg attatccctt
     4021 cgctgttgac ctggcaaagc aggaagcaca atcctcaggc gggttaccgg gtgaaaggtg
     4081 gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt
     4141 tgattgcggc aggcttgtta ccagaagtgg ggtgattaga tagcctcaaa ttccttattg
     4201 ggtgccagaa ttaacgctga cacccaatgt gacctcttaa tgcaggcagc actgcttaaa
     4261 tttcttacca ctaccgcacg gacaaggatc gttacgcccc ggtttctctt ctgctttgat
     4321 aggttgctga acagcttttt cctgcggatg cgccatccag tatgcatgta aatcaagcgc
     4381 cgccagtcga atagcatcta cgctctcttc aaacgcttct ggcgacatct tctctacccg
     4441 ctcgaagttt tcctcagtac cgtgcagcgc aatcgcatcc agcgctggtt ttaacgaatc
     4501 gggcaacgtt gaccagtcag aaagtgccac gccccgcata tagccaaagc accactcctc
     4561 aacaatcgtc agctcgctgc catcgacttc tcgcaagccg aataacggct caaactgctc
     4621 cgggaattcg ttcagacgct ctgcggtatc agccatatgt tgaaaagcca ggttcataaa
     4681 gcgcgtcatc tctttctctg acgcccagcg cggcacatag tcagccccac cccacacggc
     4741 aaccagccac tgtgccggtt caatctcttg cggagaactc aacaccgccg tcaataaacc
     4801 gtccagctcc gccacatcaa gaatggcgtg gtcagtgttg tatttggtca gaatatcgtc
     4861 cagccattcc aactcacttt cgtttaacgg tcctgttttc atacgctttt ccttgtggat
     4921 ctcaactcgc cagcacctat cttacatgcc ggtccgtatc agagatactt tttgagtggc
     4981 tttgccggtg attaaaaatt aaggagggtg taacgacaag ttgcaggcac aaaaaaacca
     5041 cccgaaggtg gtttcacgac actgcttatt gctttgattt tattcttatc tttcccatgg
     5101 tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttta aatcccttgt
     5161 gtctaccgat tccaccatcc gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac
     5221 cggactagac ggatttgcaa tccgctacat aaccgctttg ttaacgcgcc aaattcttca
     5281 ggcctttcag ccagacatcc gctgccgccg atgtctttta aactggagcg ggaaacgaga
     5341 ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgca
     5401 ctcatcaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
     5461 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
     5521 gatcgtttgc tgaaaattgc gccgaaacga tcactgatca agcaaatctg cacgcgcagc
     5581 gctcaaatat tgcaacattg accacagagt cagtaccgca gccacaaaga aaagtgcaat
     5641 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggctaacg ccaccatctg
     5701 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
     5761 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgctgccgg
     5821 taaggtcacc caccagctgt ggtaatgctc ggttaccagc accatggcga tagccacgag
     5881 aactttatct gcgacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
     5941 tgccagaaaa ccatcgaacc agtcagtcag cgccgcgacg cagaaaatga gcgcggcggc
     6001 aaatggcgac caggtgacag gcaaataaaa gaccaataca aagaatggga taaggatgac
     6061 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
     6121 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
     6181 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
     6241 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
     6301 ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg
     6361 ccaatcgcgt gatcgtgtga ttcatcgcga atatgctgga taacatgcag cgcgggagaa
     6421 tctggcggca aactaaatcc ctcaccttcc ggctcaaaga acagcgtttc cagcccagcc
     6481 ttacgatctg ctcctttggc aacaccaagt agcagcggat gatttttatc ccatgagaca
     6541 tccagttcgg cgaaaacatt tttcgcctgc gcaagctggc ctttgccgcc gtcaataagg
     6601 atcacatccg ggatcttact gtcgtcgata gctttaccat aacgccgacg cagcacctga
     6661 ttcatcgccg cgtaatcatc gcccggcgtg atgccagtaa tgttatagcg ccgatactcc
     6721 gcacgcagcg ggccgttggc atcaaacacc acacaggaag cgacggtttg ttcacccatg
     6781 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
     6841 agcgcggtca gccgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
     6901 gccgcattgg tacgcgcgag tttcagataa cgcgctctat cgccacgagg tttggtttga
     6961 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
     7021 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
     7081 aactgaccta cgaaggtttc caccacctcg ctcagttccg taccaccagg cactttcggg
     7141 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
     7201 atacccgcat cgaacgccac tccaataacg tcaaggtcgt cgccggtatt ggaaacaaac
     7261 tgtttttcgg tgacacgtcg caccgcctga atttggtcgc gaatccgcgc cgcttcttca
     7321 aactccagat tctggctggc agtttccata cgactaatga gttgcgtaag cacctgatca
     7381 tctttgccag acaaaaacaa gcgcacatac tcgacctgct gagcgtattc ttcttcactc
     7441 accagtcctt caacgcacgg ccccagacag cgccctatct gatattgcag acacggacgc
     7501 gagcgattgc gataaacact gttttcgcac tggcgaatgg gaaaaatctt ttgcagtagc
     7561 gccagtgttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
     7621 tgcttcgctc cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa
     7681 ggatatgatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgataaag tttgatgtag
     7741 ttgtgttcca gtaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctgg
     7801 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
     7861 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
     7921 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
     7981 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
     8041 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
     8101 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
     8161 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
     8221 ctggttcgat ctggcttaac gccatttgtt gagcgatatc agaagcaatg taacgctgcc
     8281 ctgaatagac agaacgaatc gcactaacga cttcctgcgg agccgcgcct ttgctgaggt
     8341 agcccgcagc tccggcctgc atgactttcg ctggtaaagg gttttctgta tggacggtaa
     8401 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
     8461 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgcc cggcaccatt
     8521 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
     8581 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
     8641 aaggaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
     8701 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
     8761 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttcc gggatcgttt
     8821 cacagtaagc ccggcgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
     8881 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa acattcatga
     8941 taaaaatatt tattggtcat tatattaacg tattttatag tactgttgat atcacgctca
     9001 aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc ttaacatttt
     9061 tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc tgatttttct
     9121 actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc gatgctgacg
     9181 ctgatgctgc aggcgatggg acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa
     9241 cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac ggttaagcaa
     9301 attaaacaag cctaccgtca gtaatataaa accggctgag agcgtgcctt tcagccggtt
     9361 tttgcatctg gcacgcagaa cagaaaagag atcaaattaa gccagtagcg gccgcgtaac
     9421 aagcaacctg cgtcttattc ggtgcattga tttttttctg catgtttttc tgatggaaat
     9481 tgaccgtatt ctcagagatt gacaaaatca tcgctatctc tgctgatgtc ttaccttccg
     9541 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
     9601 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtagc tgcattttta
     9661 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
     9721 agcccaacgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
     9781 cgcgcgcagc ttcccataac ggctgtgctt cgctgaataa gtcatcattc cacattaaat
     9841 ggccctgact aaagttttca gggttcagca ccggatcaat tgcgagaaaa ttttttgcct
     9901 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
     9961 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    10021 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacaacatcg
    10081 tgcgaagcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    10141 agtatcattt ataatgataa ttcaaactat
//
LOCUS       DAZFRE010000143        10104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFRE010000143 DAZFRE010000000
VERSION     DAZFRE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.144"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            370..1140
                     /locus_tag="ACHKUI_004523"
     CDS             370..1140
                     /locus_tag="ACHKUI_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6600579.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1131..1604
                     /locus_tag="ACHKUI_004524"
     CDS             1131..1604
                     /locus_tag="ACHKUI_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6600580.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1712..2251
                     /gene="dnaT"
                     /locus_tag="ACHKUI_004525"
     CDS             1712..2251
                     /gene="dnaT"
                     /locus_tag="ACHKUI_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6600581.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2254..2991
                     /gene="dnaC"
                     /locus_tag="ACHKUI_004526"
     CDS             2254..2991
                     /gene="dnaC"
                     /locus_tag="ACHKUI_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6600582.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3040..3534
                     /gene="yjjA"
                     /locus_tag="ACHKUI_004527"
     CDS             3040..3534
                     /gene="yjjA"
                     /locus_tag="ACHKUI_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6600583.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3788..6079
                     /gene="opgB"
                     /locus_tag="ACHKUI_004528"
     CDS             3788..6079
                     /gene="opgB"
                     /locus_tag="ACHKUI_004528"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6600584.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6219..7241)
                     /locus_tag="ACHKUI_004529"
     CDS             complement(6219..7241)
                     /locus_tag="ACHKUI_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO6600585.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7380..8285
                     /locus_tag="ACHKUI_004530"
     CDS             7380..8285
                     /locus_tag="ACHKUI_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6600586.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8456..10102)
                     /locus_tag="ACHKUI_004531"
     CDS             complement(8456..10102)
                     /locus_tag="ACHKUI_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6600587.1"
                     /translation="MLLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQPIF
                     GLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGYVI
                     HLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGLVA
                     LKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEPSI
                     QIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWSML
                     EYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGWLW
                     LMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFPGR
                     YLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSVQK
                     TEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNLAA
                     LNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2657 a         2590 c         2387 g         2470 t
ORIGIN      
        1 acagaaatct cattcattta atttataatt aaataatatc tcttcccgca gagtgcggga
       61 atcacacaat caatatttat tcaataaaat ctgatggttt taacttgtta acataaaaaa
      121 acgcgcccag ctatctatta tcctgactcg taaaggccaa ataacaaatt cattatagtg
      181 ataatattca tgcgatagtt tgtttacggc gagagataat tcgcttttta tcaccgagca
      241 tctcccgata accaggatta atcagcgcaa ttttgatctt aacctaccgc aaaacaaccg
      301 tgcttcatga tacaaatgga ttctcattat ctgaacaata cgcagcacgt ctatgacaaa
      361 gggcgagtta tgcaaactga gcaacagcga gccgtaacac ggctttgtat ccagtgtgga
      421 ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg atgagctttc ctcacgactg
      481 ggtcgggcgt tgggaatgga cagcgtcgag agttctatct cttcgaacgc catagtgctg
      541 acaaccatta aagatgggca atgcctgaca tcgacacgta aaaatcacga tcgcggcatt
      601 aatatgcatg tggtgactga agtccagcac attgtaattc tggcggagca tcatctgctg
      661 gattacaaag gcgtagagaa acgatttagc caaattcagc cattacgtta cccaagatgg
      721 ctggtagcct taatggttgg cctttcctgc gcctgtttct gtaaactcaa taaaggtggc
      781 tgggatggtg ccgtcatcac cttcctggcc agtacggccg cgatgtatat ccgccagctg
      841 ctggcacaac gtcatcttca tccacagatc aacttttgcc tcaccgcttt cgccgccacc
      901 accatttccg gattgctttt gcaacttccc actttcagca atacccccac cattgcgatg
      961 gccgccagcg ttctgctgct ggtgccgggc tttccgttga taaatgccgt cgccgatatg
     1021 tttaaaggcc acatcaatac cggactggca cgctgggcaa tcgccagtct gctgacactg
     1081 gctacctgtg tcggcgtagt aatggcactg acgatttggg ggctacgcgg atgggtgtga
     1141 tcgaatttct gttagcgttg gcgcaggata tgatcctcgc cgccattcct gcggtcggct
     1201 ttgcgatggt gttcaacgtt cccgttcgag cgttacgctg gtgtgcgctg cttggtgcga
     1261 taggtcatgg ttcccgaatg atcttaatga ccagcgggct gaatattgaa tggtcaacct
     1321 ttatggcctc tatgctggtc ggcaccattg gtattcaatg gtcgcgctgg tatctggcgc
     1381 atccgaaagt gtttaccgtg gcggctgtta tccctatgtt ccctggcata tcagcttata
     1441 ccgcaatgat ttcggcggta aaaatcagcc agctaggtta cagcgaaccg ttgatgatta
     1501 ccctgttaac caactttctt acagcttcat cgattgttgg tgcgttatcc atcggtcttt
     1561 ccattcctgg attatggttg taccgcaagc gccctcgcgt ataaaattgc ctcacttaaa
     1621 gcacgcggat gagagacagc ctcctctcct ccgtgtgtta ctataaaagt tatctccctt
     1681 ctcgttcatc gttccatatt tgagaaacag tatgtcttcc agagttttga ccccggacgt
     1741 cgttggtatt gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg
     1801 tgtggttgcc gtatttgcta acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct
     1861 ggctgaactg ctggcgctgg aagaaaaact ggcgcgtccg ggaagcgatg tcgctctgga
     1921 cgatcaactc tatcaggaac cgcaagccgc tcccgttgct gtaccaatgg ggaaatttgc
     1981 catgtatccg gactggcaac ccgatgccga ctttatccgc ctggcggcgc tatggggcgt
     2041 ggcactaaga gagccggtga ccgccgaaga actggcgtca ttcattgcct actggcaggc
     2101 ggaaggcaaa gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat
     2161 cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg aatacggtca gtgaacctga
     2221 cagccaaatt ccaccaggat tcagagggta acgatgaaaa acgttggcga cctgatgcaa
     2281 cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcgt tcaaaacggg tgaagaatta
     2341 ctggcctggc agaaagaaca aggggcgatc cgctccgccg ctctcgaacg tgaaaatcgg
     2401 gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc gtccactgca tcagaactgc
     2461 tcctttgaga actatcgcgt tgagtgtgaa ggacaaatga atgcgttaag caaagcgcgc
     2521 cagtatgtcg aggagtttga cggcaacatc gccagcttca tcttttctgg taagccagga
     2581 accggcaaaa accatctggc ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc
     2641 gtattgatca tcaccgtggc cgatattatg tcggcgatga aagatacctt caggaatagc
     2701 ggtaccagcg aagaacaact gcttaacgat ctgagtaacg ttgatctgct ggtgatcgat
     2761 gagatcggcg tgcagaccga atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat
     2821 cgccgctctt cttccaaacg cccaaccggg atgctgacca acagtaatat ggaagagatg
     2881 accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg
     2941 atcttcaact gggatagcta ccgtagccgt gtaacaggta aagagtatta atccccgttt
     3001 ctggcggtat actgcgacac aattcaacct tgagtaatga tgatgaaaac tattaaacat
     3061 cttctgtgct gtgccattgc cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc
     3121 tggaaagatg cgctctccag cgccgccagc gaacttggca accaaaacaa cacgacacag
     3181 gaaggcggtt ggtcgctcgc gtcattaact aacttgctta gcagcgggaa ccaggcctta
     3241 agcgcagata acatgaacaa cgccgcaggc attctgcaat actgcgcgaa gcaaaagctg
     3301 gcttcggtaa ccgatgccga aaacatcaag aatcaggtac tggaaaaact gggcctgaac
     3361 agtgaagagc aaaaagaaga caccaactat ctggatggca ttcagggttt gctgaaaacc
     3421 aaagatggtc agcaactcaa tctggataat attggaacga ctccgctggc agaaaaggtg
     3481 aaaaccaaag cctgcgatct ggtgttaaag caggggctga acttcatttc ctgaaccgcg
     3541 aaacgccgcg ttttagctgt tacccggctg acgcggctct ctcctgccct tttccctgct
     3601 cctggacggt tttacccctg ttacacggga agcgaccgcg atcaaaaaag tctgtttata
     3661 aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat atggcgacgc
     3721 cattacattg tgcttctcaa aaaacttaaa cgttggctaa acggcccggt ttagtgagga
     3781 tcgcccgttg tcagaactac tctctttcgc cctttttctc gcctctgtgc tgatttacgc
     3841 atggaaagcg ggacgtaaca cctggtggtt tgcggccaca ttaacggtac tggggctatt
     3901 tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgatg gtattaacga
     3961 cgcggttctc tataccttaa ccaacagcct gaccggtgct ggtgtcagca aatacatcct
     4021 gcctggtatc ggcattgtgc tggggctggc ggcggtgttc ggtgcgctgg gctggatcct
     4081 gcgccgtcgt cgtcatcatc cgcaccattt tggttacagc ctgctggcac tcttactggc
     4141 gctgggttca gtggatgcca gcccggcatt tcgtcagata acggaactgg tgaaatccca
     4201 gtcacgcgac ggcgacccgg actttgcggc ttattataaa gagccgtcga aaactatccc
     4261 tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga cctattttga
     4321 taacgaggct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga
     4381 tttcagccac acgcagcagc tgccaggaac ggattacacg attgcgggca tggtggcttc
     4441 tcagtgcggc ataccgctgt ttgccccatt tgaaggcaac gcctccgcct ctgtctccag
     4501 cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt atcagaacta
     4561 tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat gtgttcctga agtcgcatgg
     4621 cttcgaccac ttatacggct cagaagagct gaaaagtgtg gtggccgacc cgcactatcg
     4681 taacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa aatttgaaga
     4741 gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata cccatcaccc
     4801 ggatggtttt atctcgcgca cctgtaaccg caaaaaatat gattttgacg gtaagccgaa
     4861 tcagtcattc agcgcggtaa gctgcagcca ggagaatatt gcgacgttta tcaacaaaat
     4921 caaagcgtca ccgtggttta aagataccgt catcgtcgtc tcttctgacc atttagcgat
     4981 gaacaatacg gcgtggaaat accttaataa gcaggatcgc aataacctgt tttttgtcat
     5041 ccgtggcgac aagccgcagc aagagacgct ggcagtgaag cgtaatacga tggataacgg
     5101 cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggcc gtagcagttt
     5161 atccgggcag tcgatgtcgg aaatcttcct caatatcaaa gagaaaacat tggcgtggaa
     5221 gccggatatc atccgcctgt ggaaattccc taaagagatg aaagagttca ccatcgacca
     5281 gcagaaaaac atgattgcct tctcaggtag ccatttccgt ttgccgctgc tgttgcgggt
     5341 ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac tcagcaccgc tgcgtttcca
     5401 gctggcagat ttcgctccac gcgacaattt cgtctgggtt gaccgttgct acaagatggc
     5461 acaactctgg gctccggaac tggcactctc caccgactgg tgtgtctcgc aagggcagct
     5521 tggcggtcag caaattgttc agcatgttga caaagcaata tggaagggca aaacggcatt
     5581 taaagatacg gtcatcgaca tggcgcgtta caaaggcaat gtcgatacgc taaagattgt
     5641 tgataacgat attcgctaca aagccgacag tttcatcttt aacgtcgctg gtgcgccaga
     5701 agaggtgaaa cagtttagcg ggatttctcg tccagagtcg tggggtcgct ggtccaacgc
     5761 acagctgggt gatgaagtaa aaatcgagta caagcatccg ctgccgaaga aatttgacct
     5821 ggtgattacc gccaaagcat acggcaataa cgccagccgt cctattccgg tacgcgtagg
     5881 caatgaagaa caaacccttg tgctgggcaa tgaagtgacc accaccacac tgcatttcga
     5941 taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct caaccaacga
     6001 agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt
     6061 ggtggaacgt gaagggtaat tcctgactga tgtgtaggtc gcataaggtg cgacctacac
     6121 accttctccg gcctacaaaa cattattgag aaacagaaaa aaaacagcgc ccaacacttc
     6181 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacgccctta
     6241 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga
     6301 tagcggtggg ttaacatcat gtcggcagtg attttccctt ccgccatcaa tcgaccgact
     6361 ttagcgaaat cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc ttttttatgg
     6421 aattccggat cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg
     6481 ccgtgacgaa tcagatttac ggtgttattc atcgcatgtt gattacctgt cgcgtcaatc
     6541 actttctgcg ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa atcttcggct
     6601 gacgggtcca gaacaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
     6661 gtatccgcca ccaccacctg cgcgccatcg gctttagcga ttgccgccgc gcccagaccg
     6721 attggccccg ccccgaccac cagcacctgc tcgctgggtg caatagctgc acgacgcacc
     6781 gcatgagcgc taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct
     6841 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
     6901 acgccaatga ctgaaatttt ttcacagcaa ttggtacgac cgcttttaca cgccgggcat
     6961 tgctgacaag aaatatacgg aattaccgca acttgctgtc cgatattaaa attgtgaatg
     7021 ttttttcctg attcaataat ctccccacat atttcatggc ctaaaacgcg tggataacta
     7081 aaaaatggct gattgcccgc ccaggcatga atatctgtac cgcaaatccc gacagtttta
     7141 attttaatta atgcttcatt atcgcctggc accggtcttt cccggacttc ccatactaaa
     7201 ttctttattt cctgacaaat taatactttc attgtggaca tgattcaccg ctccttttat
     7261 tttatgactt tcagatatcg tcgaatttat aagagcgcct gaatttaccc ataaaatatg
     7321 cggatgatcg cgcataaaaa tgttttaaat cggcttttaa tgccggactg gagggaagaa
     7381 tgagtcgttc gcagaattta cgtcatagcg tcataaatca ggtgattgaa gatatggcga
     7441 ggggcaatat tccttctccg ctgccttcac aaagcggact ggcagaaatg tataacatca
     7501 gtcgcactac ggtacgccac atactccagc atttaagcgc atgtggcgtg ctcaccctgg
     7561 tggggaaaaa ttatgttatc gcccgcaaac ccgaacagaa cgatggattt gattgcatca
     7621 cggcctcgct gaaagagcaa aaccgtcttt tcgagcaggc atttttcacc atgattaacc
     7681 tgcgccagtt acgcgccgga gaaagctttt ccgaacttca gctggcgcga gcggcaagcg
     7741 tcagtccggt agtggtacga gaatatcttt tgaaattcgg tcgttataat cttattcaaa
     7801 gcgaaaagcg cggccaatgg agcatgaagc agttcgatca atcctacgcc gagcagctgt
     7861 ttgagctacg cgaaatgctc gaaacacatt ccctgcaaca ctttctcaac cttcccgatg
     7921 atgaccctcg ctggctgcaa gcaaaaacca tgctcgaacg ccaccggacg ctgcgcgaca
     7981 gcattggcag caactttcga atgttttctc aattagaccg tgattttcat gccatgctgt
     8041 tatccgctgc tgacaacatc ttttttaatc aatcacttga aattatatcg gtgatctttc
     8101 attttcacta tcaatgggat gaaagcgatc tcaaacagcg caatatcatt gctgttgatg
     8161 aacatatgac cattctcagc gccctgattt gccgtagcga tttagatgca ataacggcgt
     8221 tgcgaaacca tctggatacc gccaaacaat caatgattcg ttccatcagg caacatcacc
     8281 gataaattaa gtacagatta aaaacattga ttcttggttt atatcactat gttgtattaa
     8341 tgcagtatta atccaggcga ttgttctgcc cataaggaaa tacataggga ataaacgatg
     8401 ctggaagtat tactctcagc catcatttaa ttccctatta ctggcagcga taaaattatc
     8461 ggctatccat gattaacgtt tccagcacat ctaattcact gactattttt gccagtgttt
     8521 catcactgac ttcttgcctt gcgcgcatct ggtaaatagc cgcgcgtttg gcatttaacg
     8581 ccgccagatt aaatgttctt tccagcttcg cacgctgcat atgcagggaa ttatcattag
     8641 gcgacatatc ctcttcacta tgtcgcatat aaccgataac tctggccccc gtttccataa
     8701 tcaactcgtt atcgaggttt tctgttctgg cgcgcagcaa acgttgctcg gtcttttgca
     8761 cactcagtat ggccatttta gccataaaca gatcggcagc ttcctcttct ttttgtcggt
     8821 tatcgctgaa aatccccaca tttttcccca gtaataacgg cagaataata atgccgcaaa
     8881 ccagcgaaaa gaggatcaca cctgtggcga gaaataccag caagtaacgc ccgggaaaag
     8941 gtgtgccatc cggcagcaat aacgggatag aaagcacccc cgcaagcgtg atagctccgc
     9001 gcacccccgc aaaggtcgcc agcagcatat gacgcgtttt atatccagca aacagcatcg
     9061 gatgtttgcc gggtcgttta cggctataca gacgcatcag ccaaagccag cccaggcgca
     9121 caaccattaa cgccaggtag atccaaatga catcaaggag cagcgatccg gtgtccacca
     9181 gtggatcgtt ttctgcacgg atcagtgatt ccgaaataat gcccggcagt tgtaagccta
     9241 aaatcagaaa caccatgccg ttaaagacat attcaagcat cgaccagcta cttttagctt
     9301 taattctcag gcgaataggc gcgtggtgga ctacgccaat gcgactggtt gttaaccccg
     9361 cggcgacagc ggcaagaatg ccagataagc caaaatgttc cgcaaccaga taaacaataa
     9421 acggcaacag aagcatcaat acaatctgaa tggaaggttc atcgtgagca tatcggctga
     9481 tcaacagcag ccctttacca tataaccagg taacagcgat ccccacggca ataccgccca
     9541 acgcaacgac aaaaaacgca atggttgcgc catgaacgct gaattccatc gttcccatcg
     9601 tcactgcaac agcaaatttc aacgccacca ggccggaagc atcattcatc aacgcttcgc
     9661 cttcgagcac ttcctggatg tttttgctga tacgcccttt cccgacaatc cccagcaacg
     9721 ccacggcatc ggtaggcgac aacacggcgg ctaatgcgaa agcagcgatg agcggcacat
     9781 tcggtaagag taaatgaatc acatagccga caccaaaaat agtgattagt accaacacaa
     9841 gtgccaggct gataatttcc cggctgtgct tgatcaattc tttaggttgt atttttgaac
     9901 tttcagcaaa caacaggggg ggtataaaca ataagagaaa aagttcagga ttgaattcca
     9961 catgcagacc aaatataggc tgggccagta ctgcgccagc ggcaatctgg attaaaggaa
    10021 gtggtatctg gacaggagac agacgtgtaa gcacgctggt taatgatact accaacgtaa
    10081 gaattaaaat tgttaaaagt attt
//
LOCUS       DAZFRE010000144        10061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFRE010000144 DAZFRE010000000
VERSION     DAZFRE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.145"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(48..167)
                     /locus_tag="ACHKUI_004532"
                     /pseudo
     CDS             complement(48..167)
                     /locus_tag="ACHKUI_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            245..1339
                     /gene="ddlA"
                     /locus_tag="ACHKUI_004533"
     CDS             245..1339
                     /gene="ddlA"
                     /locus_tag="ACHKUI_004533"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6600588.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1363..1575)
                     /gene="yaiZ"
                     /locus_tag="ACHKUI_004534"
     CDS             complement(1363..1575)
                     /gene="yaiZ"
                     /locus_tag="ACHKUI_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6600589.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1835..2143
                     /gene="yaiY"
                     /locus_tag="ACHKUI_004535"
     CDS             1835..2143
                     /gene="yaiY"
                     /locus_tag="ACHKUI_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6600590.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2202..3296)
                     /gene="yaiW"
                     /locus_tag="ACHKUI_004536"
     CDS             complement(2202..3296)
                     /gene="yaiW"
                     /locus_tag="ACHKUI_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6600591.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3309..4529)
                     /gene="sbmA"
                     /locus_tag="ACHKUI_004537"
     CDS             complement(3309..4529)
                     /gene="sbmA"
                     /locus_tag="ACHKUI_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO6600592.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDKVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4881..6038
                     /gene="ampH"
                     /locus_tag="ACHKUI_004538"
     CDS             4881..6038
                     /gene="ampH"
                     /locus_tag="ACHKUI_004538"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6600593.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGTRVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6039..6662)
                     /gene="iprA"
                     /locus_tag="ACHKUI_004539"
     CDS             complement(6039..6662)
                     /gene="iprA"
                     /locus_tag="ACHKUI_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6600594.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRNGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLCREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6748..9735)
                     /locus_tag="ACHKUI_004540"
     CDS             complement(6748..9735)
                     /locus_tag="ACHKUI_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6600595.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGAITSELPFGYYSITPAEVNAKGEVVTPQTYNGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTAQANYGYGVVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2643 a         2492 c         2335 g         2591 t
ORIGIN      
        1 ctcttcgtta tcttataatt aagagaaaaa aactcaccac atggtcattt aagccaggaa
       61 taaattagct tcccgttcat catttttgtg atcttgcaca catctttgag ataacgcact
      121 ttcttcacca gtacgttgct cgctttgtca gcagaagatt gcaacattct gtcagaaggc
      181 cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg tgttagacgg gataggtttt
      241 taagatggaa aaactgcggg taggaatcgt ttttggtggt aaatcagcgg aacatgaagt
      301 gtctctgcaa tcggcaaaaa acattgtcga tgccattgat aaaagtcgct tcgatgttgt
      361 gctgctgggc attgataaac aagggcaatg gcacgtcagc gatgccagca attatctgct
      421 aaatgcagac gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt
      481 gccaggtaaa catgagcatc agcttattga cgcacaaaac ggtcagccgt tgccgacggt
      541 ggatgtcatt ttcccgattg tccacggtac gctgggcgaa gatggttcct tgcagggaat
      601 gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt ctggcttcag cggcctgtat
      661 ggataaagat gtcaccaaac gtctgctgcg cgatgccggg ctgaacattg cgccatttat
      721 taccctgacg cgcgctaatc gtcacaacat cagttttgcc gaagtggagt ctaaactggg
      781 gttaccgctg tttgtaaaac cggctaatca gggctcttct gttggtgtca gcaaagtaac
      841 cagtgaagaa cagtacacaa ttgccgtcga tctggcgttc gagttcgatc ataaagtgat
      901 cgttgagcaa gggatcaaag gtcgtgaaat cgaatgcgca gttcttggca acgataaccc
      961 gcaagcaagc acctgtggcg agatcgtact caccagcgat ttctatgcct acgacaccaa
     1021 gtacattgac gaagatggcg cgaaagtggt agttccggca gccattgcgc cagaaatcaa
     1081 cgataagatc cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg caggcatggc
     1141 gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga tcaacaccct
     1201 gcctggcttc accaacatca gtatgtatcc gaagctgtgg caagccagtg gtctgggtta
     1261 caccgacctg atcacgcgtt tgattgagct ggcgctggag cgtcacgctg cggataacgc
     1321 actgaaaacc acaatgtaat attcaaacgc ccggtaatat tcttattggg cgttttcagt
     1381 ttcttcatcg cgacgacgaa taatcaaccc cgccagccag aaactaatca cccatgtcac
     1441 cagaccgacg gcataggtct gccaaccttt tgcttcaaat cccagtaacc ccaccacgcc
     1501 attaagaatg aatataaggc caatggcgaa cgcatagtag tgccagtcac ggcggatttt
     1561 tacaggcaga ttcatggcag ctccgttaac gcaaaacaac atccttgcat attctgtaac
     1621 aactgtctgt ggtgtagaca acactttcgc aattgttaat aaatttaact taaggttgct
     1681 attttgtgac tcacaacgaa gattcacgct tcgggaatat tcccaacatc aatttaccat
     1741 gattctgata ttgacaatct aaatgacctg tcagacttgc cataagccgc tggtatcaca
     1801 ccagaagaga agcagatgac ttccggaggt ttttatggct gatttcaccc tgtcaaaatc
     1861 gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct ggcaatattg cctatgcgtt
     1921 gtttgtgctg ttttgctttt gggctggggc acaattgctg aacctgctag tgcatgcgcc
     1981 cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg
     2041 tttaggtgtc ggcaccattt ttgggctgat cccgttttta gtaggctgcc tcatttttgc
     2101 agtagtggcg ctatggctgc actggcgaca tcgccgccag taagatttac gttgccagat
     2161 gcggcctaaa acgccttatc tggcgtgaca ttcctgtttg atcactgttt catacaacgc
     2221 gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag tcagattgcg ggtgattttc
     2281 gggctttcca gttgaatgcc aggcaacatt tcgcggggta gtgatttacc ggttttcgct
     2341 tcggcaagtt gataaacttt cttgtacagc gccgtctcct caaaagagaa gctgtcccct
     2401 ttctctaact gacggcgaat ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc
     2461 gttgccagtt cggttttccc gggctcttta ctgtcgtaac gaattaaatc accgtccagt
     2521 gccagcttca cgccgctggc cttactgacc gcgttctgaa acgcggcatt acggctggcg
     2581 taccagccag cgttaaaatc agcaaaacgg tatatcggcg cgctatagct agcgggataa
     2641 ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc gactgaagac ttcctgacgg
     2701 actgtaccgt ccattttcca cggataccct ttggtatgct gttcggcaaa agcaatactg
     2761 acctgcatcg gcccaccggt gcgcaccgga ttgagcgaac caaacagtgt ctgccccatt
     2821 ggcaccatgc tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa
     2881 tctaaccgtt cgctatagct tttgccgttt ggcgatttga tttttagcgc ggtatgcacc
     2941 agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg
     3001 cttaagcccg gaacggccgg atctgcctga taatttgact cctgctgcgc caccgccagc
     3061 accgagcaga cgttttccag cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga
     3121 tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg cacgacgctc
     3181 gccacatcta tcgctttttc gccctttttc aacggttgcg gagcctgact actacacccc
     3241 gctaaaacca gaaccgccaa caatgacagc gaagatagag gattgacgcg cgacattact
     3301 tctccttttt agctcaaggt atgggttact tcctgaattt tgtcaccatc cagctcatgt
     3361 tcaaagctgc gcagacgttt gtagatagac atcaactcaa ccagtgtggt ccatgagtta
     3421 atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt tagtaatctg cgtcatcagg
     3481 ccgagtgtaa tcgtaccggc aacaattgac ggaaacagca agaacaaacc gaaaacctta
     3541 tcgacctgca aatagagaat gcgggcgata ttgaaataca tatagtgaaa atagaggcgg
     3601 aaatagtttt tccgtacggc gctaaaaagc tcgcgtaccg taggcggcgt cgcgcgcgtg
     3661 gcatcgtctt caccataaac cagctcttta cggtaggcag cctctacacg ctggttttta
     3721 aactccagcc ccggcagttt gatccctacc actgccagta atccggtccc catcagcgac
     3781 cagacgatag cggcaatcac cagaccatac ggaatgtgcc cgacaatcgg cagctccggc
     3841 acatgtgcgg agagcgttac cagcaccggc aggaaggcga tcaacgtcat gatagcgttg
     3901 ataaagctga cgcccatatt ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca
     3961 cgctgcgcgg ccccttcgat atgacgcagt tgttgccagt tcgccatgta atattcgttc
     4021 atcgccgtac gccagcggaa cacgtagtga ctgacaaaga agttgttcag cacactgatc
     4081 accaccgcga tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa ttgttcgatg
     4141 gtgactttat gcggcgaact tagcgccgtt tgaatcagat catagaacgg cgcataccag
     4201 gcgttgacgg caaccccgac ttccaccaaa aaccaggtga cgaagataat cagcgcagta
     4261 ccgagtattg accagtattg ccaacggtgc gggctataga taaaccagaa caatgcaaaa
     4321 agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc
     4381 gcggcgctaa tcgggatctg cccggaagcg ccggtgatac gcgccaccca atcaccccca
     4441 ccggcttgcc agaagataac ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt
     4501 cccggctttg ggaaaaaaga cttaaacata tgcactcctg ctaacttctt atcgttttaa
     4561 caactgctgt gtattaaccg catgaccgct acaaagcgtc agccaaatga aaccgcccat
     4621 tgtgacccat agcaaggcgc ttagttcgcc agcctgacca taagaattgt ttcgacgggt
     4681 ttcacaaacc aaagattgtt tacataagcg gattaacacg cgcaggatca caaatgcagg
     4741 cattggtgag caggaaaaag atcgtgttta cgcgccgtta cgtttttaac actattattg
     4801 cactaattcg ccctttgcaa tccatcaatg agtagtataa atacgctcag ttaccttcat
     4861 tcaatctatg gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg ctgtatgcgg
     4921 cgtcattgac ctccgtccat gcggcgcagc cgatcactga accggagttt gcctctgata
     4981 ttgtcgatcg ttatgccgat catatttttt acggcagcgg tgccacgggg atggcgctgg
     5041 ttgttatcga cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga cctggtaata
     5101 acgttcgccc gcagctggat tccgtcatac gtattgcttc cctcaccaag ttaatgacca
     5161 gtgagatgct ggtgaaattg ctcgaccagg ggaccgtgaa gttaaacgat ccgctaagta
     5221 aatatgctcc accaggcaca cgtgtaccga cctacaatgg gacgcccatt acgctggtga
     5281 atctggcaac ccataccagc gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc
     5341 cggtctttgt ctggccaacg cgcgagcaac gctggaaata cctttccact gccaaactaa
     5401 aagccgcgcc gggcagccag gccgcgtact ccaacctcgc attcgatctg ctggcagatg
     5461 cactggcgaa tgcgtccggc aagccttata cccagttgtt tgaagagcag attacccgtc
     5521 cgttggggat gaaagacacc acctacaccc cctcaccgga tcagtgccgc cgtttaatgg
     5581 ttgctgagcg cggtgccagt ccgtgcaata acacgctggc ggcaattggc agcggcggcg
     5641 tctattccac gccaggcgat atgatgcgct ggatgcagca gtatttgtca tctgattttt
     5701 atcagcgcag taaccaggct gatcgcatgc agacgttgat ttatcagcgc gcgcagttta
     5761 caaaagtgat tggcatggat gtccccggca aagcggatgc gcttggttta ggctgggtat
     5821 atatggcccc gaaagagggt cgtccgggga ttattcagaa gacaggtggt ggtggcggtt
     5881 tcattaccta tatggcgatg atcccgcaga agaatattgg cgcgtttgtg gtggtcactc
     5941 gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat caatgacctg gtgaccgagc
     6001 taagcgggaa taaaccgttg gttatccccg catcctgatt aatactctga aggcaaacgg
     6061 ttgatagcga ccagtttgcc tttattcatt tcgatatagc cgcctttacg caaagcagcg
     6121 agaacttccg caacgacaga acgcgatata cgtgtacgtt gatggatata attcatcacg
     6181 ccaatacgtg atcgcaattc ttcattccag tcaatcattg ataataacgt cgcgcggatt
     6241 tgttcgtagg aattatgccc aatgagctga acgtcgcgta attccagaat tctattttgc
     6301 caggctaacc agtaaaaagc gtctcgccag agttgatttt gttcaataag agtaatggtt
     6361 tgtttggctg gcaaatgata tcccgtacag tttccttctg atattaattt gtatggtata
     6421 tcgtttttca ttaaaccatc agccagcccc ataatataag gagcctgtgt aataccgata
     6481 agtacgtttt cttctctaca caaagagata acgccctcta aaataacgaa agtatcttcg
     6541 ctattgacat cactggaaaa tataacttgc ttttcattat taaactcgaa gcgcgtaccg
     6601 tttctggaca aacatttatc gagcttacca aattcctgaa gaggtttaac tacagataac
     6661 atttgcgcgt cctttgcagt aatgcccgtc aaatccttga cgggcattat ttagattaaa
     6721 ttacctattt cttcggagtg aagaatatta ccaggtatat ttaacaccca cattcgcgga
     6781 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
     6841 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagaccctt cagtaccgtt
     6901 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
     6961 tttgaagtac ggagtcagag cctggtcttc gctgtaggtg aaggtataac ctgcatctac
     7021 acccagttca taacgcatgc tgtcgtaaga ctgaccatca actttcatgt cgttgctcag
     7081 ctggtaatca tcaccagact ggaacagacc agaaacgctg ccgtaaggag tcacgtaacc
     7141 tgcatcaccc agtttgaagt cgtaaccggc tttcaaacca aagccccaag cgtcggagtt
     7201 ggtgctgccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
     7261 agagtagctc aagctaccgt caacaaagac gttgttcgcg aagtgagcag aagagtagat
     7321 gtaggcagtc tggctgtctt gatccacctg accagaacgg tcattcatat cacctttagc
     7381 gaagcctgca gccgcaccga cgatccactt agcgttgtta ccgtcaattt tggtatcaac
     7441 accgaccatg atgccgttaa catcctgatc atagttgatg gtgccgttgt cgccgttgaa
     7501 gttaccaccg aagtagctta cccatgcgcc gccgttatca gccagaccat gacgagagtt
     7561 ggtcagacga gtaccaacgg tgtcttgttc caggttccag atattggtgt tcgcagacgg
     7621 gatgctcagc gccatgttag cgtagtcggt cagctccatc tgttgcagaa caacggtgtt
     7681 accacgctgc tcagcctgat aggtgtatgc acccaggtca gctttgttag cagcagagaa
     7741 ggtcgcgttg ctgttgacgt cgtttacata gataatttct ttatctttgt aatcagcgat
     7801 tgcgccagcg ccagttgcgt tgtcgatacg aactttgtag ttaccggttg ccgcaacaac
     7861 gttgtctaca gaactgttat ccagttcagt accgttgtct acgtcgccgt taccgttgat
     7921 agtcagatga ccatcagagt tcatagcaac aacaccgtag ccgtagttag cctgagcggt
     7981 atcccaagta cggtcgttca ccaggtcagc gttcagaaca taatcgctgc tgttgatatc
     8041 gaacacacca gaaactacat tgccgttgtg gtcagcgatg ttgccgctag tcagttccag
     8101 tttgtcagta cggaaagctt cagtatcaaa ctgatcaacg ttcacatcca gtacaccacc
     8161 gttagttacg gtgatggtat tggcatacag gttagaatag ttattccagc cagtagattc
     8221 ggttaaatta acggtactgt agctatcaac agtcaggtgg tctgtagcaa ccaaaccatc
     8281 ttcaccaata ttcagtgagg agccgttggt taaaccaatg gtatctgaga ccagagagga
     8341 atcagtaaca tttacctgag agccattatt tacagctaat gtatcaatta ctgaactatt
     8401 aacggtattc cattctgaac cattgttcag tgctacgtta aatacgccgc tctggaatac
     8461 cttgttgtca ataatacgac cccaatcgtt agcttcgata gtggcaccag gggtcaggct
     8521 gttcggcgca acatcataca tatctgccgg agcaattact tcagcactca tgtttgctga
     8581 gccaacccac ttactaccgt tgttcagagt aatgttcagt tcatcaacgt tctgagcatc
     8641 atctacccaa ccaccgttgg tatcaaggac accgtcacca ttgctgtcat ggccggttga
     8701 atagaagaat acgccatcgt tattccagtg gcttgtgaac tcgacatcac ccaacagggt
     8761 tgaatggtca aagtttacat tgtttttcat ggaataattt gagccagcat tgtcagagaa
     8821 tgacagagct acgtcatttt cgccaccatt accagcgtaa tcacttggtt cagcagattt
     8881 accgaagaag ccattgcttt cttccagcgg agtctgtgac cctgaagtta cagtggagtt
     8941 agacacatta atgttgttgt cccactgatg atattcgcta gtataagtcg tgttgcttgt
     9001 attgccttca gacagcgcaa taccagctac acgggagttg ttagtaatat tgatattgct
     9061 ttcaacatca agatttactg caacacccag agcggtatag aacgtttcat tggttgactt
     9121 agatttatca ccatcaagat atgtgtcagt gaagtaaaga gcttcatagt catcatcaat
     9181 ggtggaattg gatacgttca gagtgaatac gtcaccatcg taccaattgt agtcaaggtc
     9241 agtagtgatt ccgttataga aattatagcc attataggtc tgtggagtta caacttcacc
     9301 tttagcatta acttctgcag gagtaatgct gtaataacca aatggcagtt cagaggtgat
     9361 tgccccatga attactgaat tgctgatatc tacctggtta acagaaccgc tagcgtaatc
     9421 atgattcaag taataagtag aaatggtacc gttaacggta tcgcctttaa agacggtatc
     9481 gaaatcgcca ttggcattgt tatagtaaac aaaaccattg taagttacgt agttaccgtc
     9541 tgaacccatc gggtccatgc cgccagcatc ggcaattcca tcattgtcag tgtcatacgg
     9601 ccaaactaaa tcggtcgtgg tgttgtcatg atacccccag gtatcatagt ttgctgcatt
     9661 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
     9721 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
     9781 caatgcatag aaaacatatt atgtttcgcg tttacgatta tgcggcgtta tagaggaatg
     9841 gttcaattat tctttcccct aagtccgatt gcggacatat ataaagcacg tgaacacaca
     9901 caaagaaaga aataacaaaa acacaatatt tcaatacgtt aatgaaagcg ttaattttaa
     9961 aacatagacc atgaaaatat aaaacaacaa aaacagacaa ataatgtatc aataagcaat
    10021 gaactttaag ctattcactt tttggaaacg tcaccaaatc c
//
LOCUS       DAZFRE010000145        10047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFRE010000145 DAZFRE010000000
VERSION     DAZFRE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.146"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            204..356
                     /gene="hokA"
                     /locus_tag="ACHKUI_004541"
     CDS             204..356
                     /gene="hokA"
                     /locus_tag="ACHKUI_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6600596.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(333..404)
                     /locus_tag="ACHKUI_004542"
     CDS             complement(333..404)
                     /locus_tag="ACHKUI_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600597.1"
                     /translation="MRNTVRKAGIISRRRRLLFQFAG"
     gene            complement(544..756)
                     /gene="cspA"
                     /locus_tag="ACHKUI_004543"
     CDS             complement(544..756)
                     /gene="cspA"
                     /locus_tag="ACHKUI_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6600598.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1037..1327)
                     /gene="yiaG"
                     /locus_tag="ACHKUI_004544"
     CDS             complement(1037..1327)
                     /gene="yiaG"
                     /locus_tag="ACHKUI_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6600599.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1761..2471
                     /gene="yiaF"
                     /locus_tag="ACHKUI_004545"
     CDS             1761..2471
                     /gene="yiaF"
                     /locus_tag="ACHKUI_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6600600.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="ACHKUI_004546"
     CDS             complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="ACHKUI_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6600601.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLRPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTAHRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3599..4258)
                     /gene="yiaD"
                     /locus_tag="ACHKUI_004547"
     CDS             complement(3599..4258)
                     /gene="yiaD"
                     /locus_tag="ACHKUI_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6600602.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTSEGKAQNRRVEITLSP
                     L"
     gene            4411..6744
                     /gene="bisC"
                     /locus_tag="ACHKUI_004548"
     CDS             4411..6744
                     /gene="bisC"
                     /locus_tag="ACHKUI_004548"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6600603.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6713..7153)
                     /gene="yiaC"
                     /locus_tag="ACHKUI_004549"
     CDS             complement(6713..7153)
                     /gene="yiaC"
                     /locus_tag="ACHKUI_004549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6600604.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7150..7713)
                     /gene="tag"
                     /locus_tag="ACHKUI_004550"
     CDS             complement(7150..7713)
                     /gene="tag"
                     /locus_tag="ACHKUI_004550"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6600605.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7871..8569
                     /gene="yhjY"
                     /locus_tag="ACHKUI_004551"
     CDS             7871..8569
                     /gene="yhjY"
                     /locus_tag="ACHKUI_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6600606.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8798..10000
                     /gene="yhjX"
                     /locus_tag="ACHKUI_004552"
     CDS             8798..10000
                     /gene="yhjX"
                     /locus_tag="ACHKUI_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600607.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2398 a         2407 c         2591 g         2651 t
ORIGIN      
        1 tttttaggat tatatcaagg agaagaaacg aacttattaa gctagaatag ccacgggtgc
       61 ttgagactgt ttgtctcagg tattcaccga aaggcagaca gagaaaagcc ccacctgact
      121 ataaatcaaa gtgaggcccc tctatgcata gacaccataa ggttagcctc ttactcgttg
      181 gaaatcaaca cagggggctg ggaatgccgc agaaatatag attactttct ttaatagtga
      241 tttgtttcac gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata
      301 ttaaacagga gagttatgag ctggcggcgt ttttagcctg caaattgaaa gagtaagcgt
      361 cttcggcggg aaattattcc cgcctttctt acggtgttgc gcattctcat tgcacccaaa
      421 tttattcttc acaaaaataa taatagattt tattacgtga tcgattattt atttcctgaa
      481 aaacaaataa aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag
      541 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      601 ggacactttc tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg
      661 tacgaacacg tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      721 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      781 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      841 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      901 tgtacgtcaa accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt
      961 tttcatattt atgattaatc ggttggaaaa cggtcctgtt atcaggaccg taaacagcaa
     1021 taaagtggat aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca
     1081 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1141 ccatggcgac tgatacgccc aaaacccggg caaaatcatc gatttttaat cctgtccctt
     1201 ttcgtaactg ctcaatttcg gtacaggatg ttgttctgtg cgtcagggta attttctgcg
     1261 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1321 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1381 agaatagtcg ggaaaaacag acacagcggg gtgttgtcag ggcatactct gactatgatt
     1441 cgattcagcc agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1501 tatctcttac cactcccggt ttgtaaagat ttcgaaaatc taacgttctg cgaaggcgta
     1561 aatctctggc tacaaaaaaa gcaacggcaa tatcattttt taacaatgaa acaacaaatt
     1621 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1681 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1741 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1801 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1861 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1921 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1981 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gttgtagaca
     2041 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2101 tgaacggttc gctgggtgtg ctggcgcaac agctgcaaaa tgcgaagttg caggccgatg
     2161 ctgcacactc tgcgttaaaa cagagtgatg acctgaaacc ggtttttgat caggcattca
     2221 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2281 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2341 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2401 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2461 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttactccacg cagtcgcggc
     2521 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2581 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2641 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg aaagtggctc
     2701 ttgttcgaag acatccagcc cggcggcgtg aatttcccct ttctgcaatg cagcaatcag
     2761 tgcattttca tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2821 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tccgttaacg gcaggatcag
     2881 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2941 ttcttctgct tctttatggt ggcgacgtgc gttatagagg atgggcatgt tgaagccaaa
     3001 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3061 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3121 tacccgttct gctacctcca caacccgatg agcggtagac aacaccagcg ccatcagcgt
     3181 atcggcgacg gtttctgtta atacggttgg tgtgtgcatc agcagaattt ttcgggcggt
     3241 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3301 cggcattttt tccagcaatg cggcatcaac attctcgttt gaacccagca agccttcagc
     3361 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg cggaggtttg ccacctggtg
     3421 aacggtgaaa tgttcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3481 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3541 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctgacat gaaagggatt
     3601 acagtgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gaggtgctgt
     3661 tgctggcgat tgggttagcc gggccaaggc cttgagtacg gatgcggctg gcgtccacgc
     3721 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3781 ggtcgtgact ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3841 ctttcagtac cattgccacg ccggtcaggg tgttagcgcc cgccggtttc agggttgcgc
     3901 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3961 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     4021 aataaccaac gccgccgccc agagctgcgc ctgctgctgc gccaatgagc gcgcctttac
     4081 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gccaacgaga gagcccagac
     4141 ctgcgccgat agcagattta cctgcttcgc gttcgccggt gtaagggtta gttgtacagc
     4201 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4261 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4321 gtgagcatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4381 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4441 ctgactgccg cccactgggg gcctatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4501 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4561 gttcacagca atacgcgggt gcgatttcca atggtgcgca aaggctttct tgcgtcaccg
     4621 gaaaatccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4681 ctggagctta ttcaccatca acataaacgc attcgtgagg cttatggccc ggcatcgatt
     4741 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4801 caacgctata tggcgctggc aggcggttat actgggcatc tgggggatta ttcgaccggc
     4861 gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc
     4921 agttggccgc tggtgctgga acatagcgat gtcgtggtgc tgtggagtgc taacccactc
     4981 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     5041 ctgcgtgaca gcgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccatcgat
     5101 ttctttggcg ataaaatgga gtgggtggca ccacacatgg gcaccgatgt tgcgttgatg
     5161 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5221 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggtatagcg
     5281 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5341 gcggctattt tccaccaaaa tacaaccatg ctgatggcag gctggggaat gcagcgccaa
     5401 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     5461 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5521 cgccgcgctg cggtgctctc ttccatgcag ggtagcttgc cgggtggcac cgatgcggta
     5581 gataaaatcc ctgtcgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5641 cataacggta tggaccgaca tttcccggat attcgtttta tctggtgggc gggtggtgcc
     5701 aactttactc atcatcagga taccaatcgc ctgatccgtg cctggcaaaa accggagctg
     5761 gtggtgatct ccgaatgctt ctggacggct gcggcgaaac acgcggatat cgttctgcct
     5821 gcgacaacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5881 ctggtgccga tgaagcaagt ggtgccgcca cgcgatgaag cgcgtaatga ttttgatgtt
     5941 tttgccgact tgagtgaacg ttgggagaag ggcggttatg cacgttttac ggaaggaaaa
     6001 agtgagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6061 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6121 gaaaacccgg acagtgagcg gtttattcgc ttcgccgatt ttcgccgcga tccgcaggcg
     6181 catccattaa aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac
     6241 gcttatccgg attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6301 gccgaaccgg aacagttgca ggtactttct gctcatccgg cgcatcgtct gcacagccag
     6361 ctgaattaca gttctctgcg cgaattgtac gcggtggcaa atcgtgagcc tgtcaccatt
     6421 catcctgacg atgcccagac gcgcggcata caagatggcg atactgttcg gctgtggaac
     6481 tcacgcgggc aaattcttgc cggcgcggtc attagcgagg gaataaaacc tggcgtgatt
     6541 tgtattcacg aaggcgcatg gccagatctg gatttaaccg ctggcggcat ttgtaaaaac
     6601 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6661 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6721 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6781 aatcgacaat gtgaaaaccc tgcgcatggt agaaatctat cgccggttga tttttttgat
     6841 aaacctccag catcagatgg ggatggcgct gctgcacata ctgcatcagc gccttaccaa
     6901 taccgcgcct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6961 tgctgacaaa accgagaagc ttaccgtctt cttcccagac ccagttttgc gcgttggcaa
     7021 gatacgcatc ccgcaccagc ggaatgcagt cacgccagta actcgatttt ataaagggat
     7081 gcccccaggt tgtactttcc agccacagtt ggaggatcgc ggggagttct gaacgttgca
     7141 cttcccgaat catggtttat ttcccggatg gcaacagcag ccaaccacat gatcattcac
     7201 cagcccacat gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg
     7261 tttttttagt gccttagata gggcgtcgga ggcgggtgta gatgtgggga tttcgctcaa
     7321 cgttgtggct tgtgtcacct gtggttgatg attgacaaac gaccagacaa agtcggcaaa
     7381 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7441 tttcccacga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7501 cattgcggca actttcaccg gatcgaactg atgaaagcat gcgcgatagt tttcgcgttt
     7561 tttgaggacg gtgatccacg ataatccagc ctgctgccct tccaggcaga tcatttcgaa
     7621 cagtttttta ctgtcagttt caggcacgcc ccattcatta tcatggtagg caatataaag
     7681 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt gcccgatcgt
     7741 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7801 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7861 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgcttgcgag
     7921 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7981 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagcgatc atcaacctga
     8041 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8101 caatctggcg gaagaaaccc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8161 tcctttatat gagcaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8221 tacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8281 tgccagcgtg agtaccttag gctggcgtgt tgactcccgg cttggcgatc ttcgaccctg
     8341 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8401 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8461 tatgttgctc aatcaaaata ttgctgccta tgctgcgcta tctcaggcag aaaataccac
     8521 taacaatagc gactatctgt atacgatggg ggttagcgcc agattttaac gttatagata
     8581 caattgaaac ccataaataa caactaatgt ggcgatagtg gggactaact tactaaataa
     8641 taaatttggc gaataattgt cgagtcattc attcctgaac tagtgcattt cattccgttc
     8701 tgatggcatt tcatgccgtt tttccccagg cataaagcgc acttcgttat ggttgtcagc
     8761 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8821 tactcgctgg ctgacactca tcggtactat cattacccag tttgctctgg ggtcggttta
     8881 tacctggagt ctgtttaacg gtgcgctttc tgccaagctg ggggctcctg taagccaggt
     8941 tgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     9001 attgcaggaa cgttttggtg ttaaacgcgt cactatggct tccggcattt tgctgggatt
     9061 aggtttcttc ctgacagcac attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9121 gttggttggt ctggcggatg gtgcgggtta cctgttgacg ctctctaact gcgtgaagtg
     9181 gtttccggag cgtaagggac ttatctctgc ttttgctatc ggttcttatg gtctgggcag
     9241 cctgggtttt aaatttatcg acacgcacct gctcgaaacg gtgggtctgg aaaaaacctt
     9301 tgttatttgg ggcgcgattg tactggtgat gattgtcttt ggcgcaacgt taatgaaaga
     9361 tgcgccgaag caggaagtga aaaccagcaa tggtgtggtg gagaaggact acaccctggc
     9421 agagtcgatg cgtaaaccgc agtactggat gttagcggtt atgttcctga ctgcgtgcat
     9481 gagtggtctg tatgtgattg gtgtagcgaa agatatcgct caaagtctgg cgcatcttga
     9541 tgcaatttcc gcagccaatg ctgtgacggt tatttccatc gccaaccttt ctggtcgtct
     9601 ggtgctgggc attctgtctg acaaaatcgc ccgtatccgt gttatcacca ttggtcaggt
     9661 gattgcgctg gtggggatgg cggccctgct gtttgcacca ttgaatgcag tgacgttctt
     9721 tgcagcgatt gcctgtgtgg catttaactt tggtggcacg attactgtgt tcccgtcgct
     9781 ggttagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggcgtca tttacctcgg
     9841 tttcggtatc ggcagcattt gtggttcgat tatcgcttca ctgtttggcg gattctatgt
     9901 gactttctac gtgattttcg ccctgctgat tctgtcactg gcgctttcta cgaccattcg
     9961 ccagccagag cagaaaatgt tgcgtgaggc acatatttag aaatatgttg ctgctgaaat
    10021 aaatacgatg agggagactt cgaagtc
//
LOCUS       DAZFRE010000146         9997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFRE010000146 DAZFRE010000000
VERSION     DAZFRE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.147"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1638
                     /locus_tag="ACHKUI_004553"
     CDS             <1..1638
                     /locus_tag="ACHKUI_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6600608.1"
                     /translation="QMQGKAQSIQDVLAGLEERRVALIRQQAAEQNKVYQSLLVMNGQ
                     HTEFNRLLGLGNELLQQRQGLVNVPLRLPEAPLDDKQQGALNNSERELTLSRLKGEAR
                     ERARLGYAADDLGFVGDAYQTARQTYINNALEAWRNNQANKSKVRGGKSEAEKTEDVY
                     NRLISQQKELIALSGQNAELAKIKYRISQGDLSAISQIRKESLLRNAAILDQQSATEK
                     LKALNEELLTPEEALLSKTKERLRLLKEASPASAEYRDVMKKISRAAVENAPEYSGVS
                     PETGGAASELFRVADAEKALKKWHKQQLDMQKQLLDEKLINEQIYADRVAEINQKNSE
                     KLQDIQNGYTTASLSMFSDLTGQSAELLKGLGQEGSAAYKALFVASKAAAVAQAIINT
                     ELAATKAMAEGGMILGIPAATAMRAVGYASVALIAGQTLAGMAHDGIERVPETGTWLL
                     QKGERVVTASTSAKLDATLEKVQQVQRERQVVSGGSIHIQNSFTGKPDDATLAAIDSR
                     NRQLVVAIRKEMAAQVISPTNEFGRALKGYYGRTRKE"
     gene            1642..2109
                     /locus_tag="ACHKUI_004554"
     CDS             1642..2109
                     /locus_tag="ACHKUI_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600609.1"
                     /translation="MTENSYPHDYLPLPLMENYSFQAASPLLRTEMTSGRARQRRRYT
                     STPTKATVNWTFTTHPQAMLFEAWYRDVLKDGANWFMMQLQTPLGDLQQFKCRFTDIY
                     QGPTLVAPRYWRYSATLELWSRAVLEGEYGEYPDFIHNSDIIDLAMNREWPET"
     gene            2106..2588
                     /locus_tag="ACHKUI_004555"
     CDS             2106..2588
                     /locus_tag="ACHKUI_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HFO6600610.1"
                     /translation="MTILNRLYASSGQEVIIETLQINMGDQQHFLCTGYDDITAITES
                     GDRVTFVACAMDIALPKRNADGTQDLQFAISNIDGVVSTAVRNALDDISIASIIYRHY
                     ISSDLSAPASPPYTLSIKSGHWTAMQVQITAGYLNVLDLAWPRYRYTLNNFPALRYMR
                     "
     gene            2599..2979
                     /locus_tag="ACHKUI_004556"
     CDS             2599..2979
                     /locus_tag="ACHKUI_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter"
                     /protein_id="HFO6600611.1"
                     /translation="MFSPDKYRSVTWLKGGRVYPELDCFGIVNEIRRDLGLPAWPDFA
                     GITKDDNGLDREARRMMQTLQRCAPCEGAGVACYTGSTVTHVGIVVCMGGLLYVAECN
                     PKSHVSIIPLARFMRRFVKVEFWQ"
     gene            2976..5678
                     /locus_tag="ACHKUI_004557"
     CDS             2976..5678
                     /locus_tag="ACHKUI_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HFO6600612.1"
                     /translation="MTIRIYPSRLPGEPLETHEHGATTLHHWMKENVRGYRSDMKHPV
                     AVEVDGESIPPQAWFDYALRPDSDVRIYPVPFGLEAATIAWIGVGISVAVAAYSLIMM
                     SNMDKGGYSSASGNGLDLNPAKANTARLGDPIREVFGRYRIYPDYVVQPVTRFDKDDP
                     TRMTVEMFLCLGTGRFSFAEGDIRIGATPVASLGKGFSYTVYRPGAVVSGDSRSENWF
                     NSTEVGGTSSGTGLDMAQTAPTSADILAESITVSGAGITFNGLENADKLPWWENKTVQ
                     LVVPASYVVTSDGDYSRITGDILEEIAPYVGMPVTLNYSGTYHTLVIASYTPHSEAVD
                     GSGGVTASITLAYDTATGVAFTGLPEGWLRLSVAHAGNRYRILSLDGSTVSVRRVLSS
                     GATDTKWPGFTARTVLDFEADGVNDNEAWMGPFLACPENETVDMFEVNFSFPNGICGF
                     NKKGKKRSHTVEWEIQYRTYGSGDGWVSCHGHYSLSNVNGLGFTERVTLPAPGLVEVR
                     CRRRNEQGRDNARDSMYWQALRGRLLNRPVSYPGVTTLGITVETGGKLAAQSDRRVNV
                     VATRIYDFGKPRSISGALHHIGKSAGLRMDATAINEMDRLYWRPRGEYFDYATTDSDS
                     VLNMLQKITNAGHAYFLFADGMASVGYEGVKPWTGIISPQEMTEDLQTAFTAPSVDDY
                     DGVDVTYINGTTWTEETVQCRTADNPAPVKLESYSLDGVTDRDRAYRIGMRRLMKYRH
                     RRLSFTTTTEMDALCYNTGDRIILTDDIPGNLTLSCLITGMKTDNGFTTFTLSEAPDW
                     TYPSPRVLIRYQDGTVSGLLEPVKVSRFRLSVPYQSAFDEILADTSVTEPPRLIFCDS
                     SRVGYDAVIEEIALQSDGTCTVTAREYRDSFYDYDNATYPGDVS"
     gene            5722..7632
                     /locus_tag="ACHKUI_004558"
     CDS             5722..7632
                     /locus_tag="ACHKUI_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6600613.1"
                     /translation="MSGRTTPEPLGAASAKVLYTNAGNLDRASNDREYEWWTDRFGVL
                     RRTWYGFEQEQHRSLVRLGLNPVGTFQAGCELTAANDIIQDDVTKLWYRWDDLMTLPK
                     VVSADSTPESSGGIQQGAWQVVDVTDVLRRDLAKSAGAGLVGTTSGVSVQSVLDDYHL
                     RLTTCERNSRVENFHTLAETCTAELLSLNAPEAYDKSITLTVNEELTTDYTGPVTGHC
                     SIGNPQNYTIAMCTSTTLEYQVSSVVLESDGTFSFARSWPGAKSFKLYRTSNNGLVTV
                     REDPLCIRSYRMPSDAGEETVRVMKDRTYTYDQAVSAIALMAQGHSQTERFVRGLCAI
                     VGSGGSEGSVPFFVNRMSAQTSSQYYRTGNAAWVAYALAYYLLKYPDGEMAVVARDKL
                     TQCAEWIEMFRVTDGSDVRSGLYTSGSGRYLDGVFYPDFKADWCTSEHQFDLWFLFDL
                     MGRVGFTGYAEKAKALADAIMEKLWVEGEGRFYAGMRTTGVDKASPLDCASWGGLFVA
                     NIDMEKARRCFTYLDRFWYATHDATGYTPYHPKYGYPNKQRGVWVEGSAGVALLARRL
                     GDDATAMDILARLAPLRTRYGYIDSSDYPDNDDMPPWPSSCNTAWMILACDPQGFWNV
                     DSPATDVGYYLY"
     gene            complement(7703..8029)
                     /locus_tag="ACHKUI_004559"
                     /pseudo
     CDS             complement(7703..8029)
                     /locus_tag="ACHKUI_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            8069..8158
                     /locus_tag="ACHKUI_004560"
                     /pseudo
     CDS             8069..8158
                     /locus_tag="ACHKUI_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8367..9659)
                     /locus_tag="ACHKUI_004561"
                     /pseudo
     CDS             complement(8367..9659)
                     /locus_tag="ACHKUI_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
BASE COUNT         2387 a         2325 c         2882 g         2403 t
ORIGIN      
        1 caaatgcagg gcaaagcgca atccattcag gatgtgcttg ccgggctgga ggagcgacgg
       61 gtggcgttga ttcgtcaaca ggcggcggaa caaaacaaag tgtatcagtc gttactggtg
      121 atgaatggcc agcataccga gtttaaccgt ctgcttgggc tgggtaatga attacttcag
      181 cagcgacagg ggctggtgaa tgtaccgtta cgattaccag aggcacccct ggatgataag
      241 cagcagggtg cactgaataa cagcgagcgt gaactgactc tgtcccgcct gaagggggaa
      301 gcgcgtgagc gtgcccggtt gggttatgcc gcggatgacc tcggttttgt gggggatgct
      361 tatcagacgg caaggcagac ttatatcaac aatgcgctgg aagcatggcg taacaaccag
      421 gcgaataagt caaaggtacg cggtggaaag tcggaagcag aaaagacaga ggatgtttat
      481 aacaggctga tcagtcagca gaaagagctg atagccttgt cagggcagaa tgctgaactg
      541 gcgaaaataa aataccgtat tagccagggt gatctttctg ccatttcgca gatcagaaag
      601 gagtcgcttt tgcgtaacgc cgccattctc gatcagcagt ctgcgacgga aaagttaaaa
      661 gccctgaatg aagaattgct gacaccggaa gaggcattac tcagtaaaac aaaggaacgt
      721 ctgcgtctgc tgaaagaggc ttctccggcc agtgctgaat acagggacgt aatgaaaaaa
      781 atatcccgtg ctgctgttga aaatgcgcct gaatattccg gtgtctcgcc ggaaactggc
      841 ggtgcggcca gtgagttgtt cagggttgct gatgcagaaa aggcgctgaa aaaatggcat
      901 aaacagcagc tcgacatgca gaagcaactt cttgatgaaa aactgattaa tgagcagata
      961 tatgcggatc gggttgctga aattaaccag aagaattcgg aaaaacttca ggatatccag
     1021 aatggatata caacggccag cctgtccatg ttttctgatt taacggggca gtccgcagag
     1081 ttactgaaag gactgggaca ggaggggagt gccgcatata aggctttgtt cgttgccagt
     1141 aaggctgccg ctgttgcaca ggcaatcatt aacacagagc ttgccgccac taaggctatg
     1201 gctgagggcg ggatgattct tggtattccg gcagcgacag ccatgcgtgc agtcggttat
     1261 gcctcagttg cgctgattgc agggcaaacg ctggctggta tggcgcatga tggtattgag
     1321 cgcgtaccgg aaacgggtac gtggttactt cagaaagggg agcgggttgt cacagccagc
     1381 acttctgcga agctggatgc gacactggaa aaagttcaac aggtgcagcg ggagcgacag
     1441 gttgtttcgg gtggaagcat tcacattcag aactcgttta ctggtaaacc ggatgacgca
     1501 acactggcag cgattgacag tcgtaaccgt cagcttgtgg tggctatcag aaaggaaatg
     1561 gcagcccagg tcatctctcc caccaatgaa tttggcaggg cactgaaagg ctattatggc
     1621 agaacgcgta aggagtaaga cgtgactgaa aacagctatc cacacgatta cctgccgctc
     1681 cccctgatgg aaaattacag ttttcaggcg gcatcacctt tgctgagaac agaaatgaca
     1741 tcgggcagag cccggcagcg gcgcagatat acgtccacgc cgacgaaggc aacggtgaac
     1801 tggacgttca ctacccaccc tcaggcaatg ctgtttgaag cgtggtaccg ggatgttctg
     1861 aaggacggtg caaactggtt tatgatgcag ttgcaaaccc cgctaggtga tctgcaacag
     1921 tttaaatgcc ggtttactga tatttatcag ggacccacgc tggtagctcc ccgctactgg
     1981 cgctattcgg ccacgctgga gttgtggtca agagcggttc tcgaggggga gtatggtgaa
     2041 tacccggact ttatccacaa cagcgatatt attgatctgg cgatgaacag ggagtggcct
     2101 gaaacatgac gatcctgaac cgtctttatg cctcatccgg tcaggaggtg attattgaaa
     2161 cgctgcagat taacatgggt gatcagcagc attttctttg taccggttat gacgatatca
     2221 cggccataac ggagtcgggg gacagggtga cttttgttgc ctgtgcgatg gatattgccc
     2281 tgccgaaacg taacgccgat ggtactcagg acttacagtt tgcgatcagc aacattgacg
     2341 gggtggtgtc aacggcggta cgcaatgcac tggatgatat cagcatcgct tcgataatat
     2401 accggcacta catttcctct gatctttccg caccggcatc accaccctac accctcagta
     2461 taaaatcagg tcactggacg gccatgcagg tgcagatcac cgccggatat ctgaatgtgc
     2521 tggatcttgc ctggccccgg taccgttata ccctgaataa ttttcctgcg ctcagataca
     2581 tgcgttaaga ggtttatcat gttcagtcct gacaaatacc gttcagtcac ctggctgaag
     2641 ggcggtcgcg tttaccctga acttgactgt ttcggtattg tgaatgagat ccgccgtgat
     2701 cttggtttac ctgcctggcc cgattttgcg ggaataacga aagatgataa cgggctggat
     2761 cgtgaagccc gcaggatgat gcagacgttg cagcgttgtg cgccctgtga gggggccggg
     2821 gtggcctgtt acacggggag taccgtcact catgtaggga ttgtggtctg catggggggg
     2881 ctgctttatg tggcggaatg taacccgaaa agtcatgttt ccattattcc gctggcgcga
     2941 ttcatgcgtc gttttgtgaa agtggagttc tggcagtgac tatccgaatc tatccatccc
     3001 gcctgccggg ggagccgctg gagacacatg agcatggtgc cacaactctg caccactgga
     3061 tgaaggagaa tgtcagggga taccggtctg atatgaaaca cccggtggca gtggaggttg
     3121 atggagaatc cattccccct caggcatggt ttgactatgc cctccgtccg gacagcgatg
     3181 tccgtattta tccggtgccg tttggtctgg aggccgccac catcgcctgg atcggcgtgg
     3241 gcatttccgt cgctgtggca gcgtattcac tgattatgat gtcgaatatg gataagggag
     3301 gatattcctc agcttccggt aatggtcttg atctcaatcc ggcaaaagcc aacaccgcca
     3361 gactgggtga ccccatccgc gaagtgttcg gtcggtaccg gatttatccg gactatgtgg
     3421 tacaaccggt aacccggttc gacaaagacg atccgaccag aatgacggtt gagatgtttc
     3481 tttgtctggg gacggggcgt ttttcttttg cagaggggga tatcagaatt ggcgccacgc
     3541 cagtggcttc tctgggaaaa ggcttcagtt atacggtata tcgtccggga gcagtggtct
     3601 ccggcgacag ccggagcgag aactggttca actccaccga ggtggggggg acatcatccg
     3661 gtaccggcct ggatatggca cagaccgctc cgacgtcggc agacattctg gcggagtcca
     3721 ttacagtatc cggtgcaggt attaccttta acgggctgga aaatgccgac aaacttccct
     3781 ggtgggaaaa taagacggtg caactggtgg taccggcgtc gtatgtggta accagcgatg
     3841 gtgactacag tcgtatcacg ggcgatattc tggaagaaat cgcgccttat gtggggatgc
     3901 cggtaaccct gaattacagc gggacatacc acacgctggt gatagcctca tacacaccgc
     3961 attcagaggc agtggacggc agcggcgggg tcactgccag tattacgctt gcctacgaca
     4021 cggcaacggg tgtggccttt acaggcttac cggagggctg gctgcgactt tccgtggcgc
     4081 atgccgggaa caggtatcgt attctcagcc tggacggaag tacggtctcg gtcagacggg
     4141 tgctgagcag cggcgcaaca gacacaaaat ggccggggtt tacggcgaga accgtgctgg
     4201 attttgaggc tgacggtgtt aatgacaatg aagcctggat gggaccgttt ctggcctgcc
     4261 cggaaaatga aacggttgat atgttcgagg tgaatttttc gttcccgaac gggatttgtg
     4321 gatttaataa aaaaggtaag aaacgcagcc atacagtgga gtgggaaatc cagtaccgga
     4381 catatggcag tggtgatggc tgggtgagtt gccacggaca ttacagcctc agcaatgtga
     4441 acgggctggg atttacggag cgggtgacgc tgcccgcgcc gggactggtg gaggtacgct
     4501 gtcgccgccg gaatgaacag gggcgtgata atgcgcgtga cagcatgtac tggcaggcgc
     4561 tgcggggcag gctgctgaac cgtccggttt cttatccggg cgtcacgaca ctgggtatca
     4621 cggtggaaac aggcgggaaa ctggctgcgc agtcagaccg ccgggtgaat gtggtggcca
     4681 cacgcattta tgattttggt aaaccacgca gtatctccgg tgcgctgcac catatcggga
     4741 agtcagccgg actacgtatg gatgcgacgg cgattaatga aatggaccgg ctgtactggc
     4801 gcccgcgcgg cgaatatttt gattatgcca ccacggacag cgattcggtg ctgaacatgc
     4861 tgcagaaaat cacaaatgcg gggcatgcat attttctgtt tgctgatggt atggcatcag
     4921 tggggtatga aggggtgaaa ccgtggacag gcatcatcag cccgcaggaa atgacggaag
     4981 atctgcagac agcgtttacg gcaccttctg tcgatgatta tgatggcgtg gacgtgacgt
     5041 atatcaatgg caccacatgg acagaagaga cggtacagtg ccggacggcg gataatccgg
     5101 ccccggtgaa actggaaagt tattcactgg acggcgttac ggatcgggac agggcttacc
     5161 gcatcggtat gcgtcgcctg atgaaatacc gtcatcgtcg cctttccttt acgaccacca
     5221 ccgaaatgga cgcactgtgt tacaacaccg gagaccgtat cattctgacg gatgacatcc
     5281 ccggcaacct tacgctgtcg tgtctgatta ccgggatgaa aaccgacaac ggtttcacga
     5341 cgtttacgct cagtgaagca cctgactgga cgtatccgtc accccgtgtg ctgatacgct
     5401 atcaggacgg aacggtgtcg gggttactgg agccggtaaa agtgagccgg ttcaggttgt
     5461 ctgtcccgta ccagagcgcg ttcgatgaga tacttgcgga tacgtccgtc actgaacccc
     5521 cacggctgat tttctgtgat tcatcacgtg tggggtatga cgcagtgata gaagaaattg
     5581 cgctacagtc tgacggcacc tgtacggtga cagcccgtga ataccgggac agtttttacg
     5641 actatgacaa cgccacgtat cccggcgatg tgagctgatt ttgtacccgc ttcggcgggt
     5701 tttacttttt atgaggtaac tatgtctggc agaaccacgc cggagccgct gggggcggcg
     5761 tctgcaaaag tcctgtatac caacgccggt aatcttgacc gggcatcgaa tgaccgggaa
     5821 tacgaatggt ggacagaccg ttttggcgtt ctgcgccgga cctggtacgg ttttgagcag
     5881 gagcagcacc gttcgcttgt cagactggga ctgaatcctg tcggaacgtt tcaggccggt
     5941 tgtgaactga ccgcagcgaa tgacatcatt caggatgacg tcacaaaact ttggtaccgc
     6001 tgggatgacc tgatgaccct gccgaaagtg gtctcagcag actcaacgcc ggaatcgtcg
     6061 ggagggatcc agcaaggtgc ctggcaggtg gtggatgtga cggatgtgct tcgccgtgac
     6121 ctggcaaagt ctgccggtgc cggtctggtg ggtaccacat caggtgtttc tgttcagtct
     6181 gttctggatg actatcattt gaggctcacc acctgcgaac gcaacagccg ggtggaaaat
     6241 tttcacacgc tggcggaaac ctgcacagcg gaacttttat cgctgaatgc cccggaggca
     6301 tatgacaaat ccattacgct gacggtcaat gaggaactga ccacggacta taccggtccg
     6361 gtcacaggtc attgcagcat cggtaatccg cagaactaca ccattgcgat gtgtacgagc
     6421 acgactctgg aatatcaggt ttcctcggtg gtactggaga gtgacggcac gttcagtttc
     6481 gccagaagct ggccgggggc caagtctttt aaactgtatc gcacatcaaa taacggtctg
     6541 gtaaccgtcc gggaagaccc actgtgtatc cgctcttacc ggatgccatc ggatgccgga
     6601 gaggaaacgg taagggtgat gaaagaccgt acgtacacct acgaccaggc tgtgtcggcg
     6661 attgccctga tggcgcaggg gcacagtcag actgagcgtt ttgttcgcgg gttgtgtgcg
     6721 attgtgggca gcggaggcag tgagggaagc gtgccttttt ttgtgaaccg catgtcagca
     6781 caaacatcat cgcagtatta ccggacggga aacgcagcat gggtggcgta tgcgctggcg
     6841 tattatctgc tgaaatatcc ggacggtgaa atggccgttg ttgcccggga taaactgacg
     6901 cagtgcgcag aatggattga aatgttcagg gtcacagatg gcagtgatgt tcgctctggt
     6961 ttgtatacat ccggtagcgg acggtatctg gatggcgtct tttatcctga ttttaaagca
     7021 gactggtgca cgtcagagca tcagttcgac ctgtggtttt tgtttgatct gatgggacgg
     7081 gtgggcttca ccggctatgc tgaaaaggcg aaagccctag cggatgccat tatggaaaaa
     7141 ctgtgggtgg aaggcgaagg gcgattttat gccgggatgc ggaccaccgg ggtggacaag
     7201 gcttcaccgc tggactgcgc gagctgggga gggctgtttg tggccaatat agatatggaa
     7261 aaagcccggc ggtgttttac gtacctcgac agattctggt atgccacgca tgatgcgact
     7321 gggtatacgc cgtatcaccc gaaatatggt tacccgaata aacagcgggg agtctgggtg
     7381 gagggcagtg caggcgtggc attgctggcc cgcagactgg gagatgatgc cacagcgatg
     7441 gatattctgg cgaggctggc accactgcgc acacgttacg gttatattga ttcaagcgat
     7501 tatccggata atgatgacat gccgccgtgg ccttcttcct gtaacacggc ctggatgata
     7561 ctggcatgtg atccgcaggg gttctggaat gtggattcac cggctactga tgtcggttat
     7621 tacctttatt aatcgcatca tcccccacga cctgaacgac ggggtctgat ggcacagcgg
     7681 ataagacccc ggtatccggg taaatcagtg tcagaccgta tttatccctt gcacgattgc
     7741 acgcctcatt gatttcgccg ttctcaccag acatggcgaa ttctctgcac gtggatgagc
     7801 gattttcata tattgtgcag caggcatttt tgccgagtgt tcccgcaaga gcaatacagc
     7861 gggggttttt ctggttagtg ccgctcatgc atcggtgaaa aggggagatt tgttctgtga
     7921 gtctggcggg gatgctgccg ccagcatcat cggcttcggc ccagtaaaaa gagacgcgaa
     7981 aaaatgcaca acaggcacca cacctcatgc atggattcag attgctcata gatataaatc
     8041 gtaaagagaa cagcggtacg tcgtatgcaa gaacgtaccg cggctggctg gataactttc
     8101 aatagtgcga gtattgaatg atttccagcc gttattgatt ttacgtgtaa atttatgaat
     8161 aaactacttg tttattgtgg aatttgtttg gggcatgtat gggacagaaa tgtctgtttg
     8221 gggacatttt ggggcatggt tgggacacta taacacatac gaacttaacc gaatttcgta
     8281 tggtgttaat ttttaacgtg ttgaataaac tagagaaagc gaatgctctt gggcgttctt
     8341 tagtgatttt taaaatagtc ctgtcatgca gaggatatgg agccgtacat catcagccag
     8401 tctgtcggtg agactgaaat cgccgccctt ggggacaggc gttcggcttt cagttctccg
     8461 gcacggggat tgaagctctt gtaccggtca tagcgggaac cgacgataat gttaaccggg
     8521 tagcttttca ttgtcatttc atcctggaaa tacaacgatg taaagtcaat gtttccttcc
     8581 ggatatagtg tggcagagcc ttctggcttc tgctgctgac ggtaatattc agcgccataa
     8641 cttaactgat gggctcccgg aaaaatcaga gtatgactcc tgttgacaac attaacaccg
     8701 gaggttctgt tgtttctcca ttctgccgtt ttgttgtgac cgtttgtctt gatagtgata
     8761 tcactgagat ataaagttga ctttacattg atgaggctgt tatccacagg agcgtactgg
     8821 taccagaact gtacgtcatt ttgtactgtt tttctgtcac gcaggccgct gtcaccatgt
     8881 gtctgagtgg gattgcccgg ttcagtggtg ttattccggt agagacgcaa gctggcacct
     8941 gctttgttac tgtctgttat accgacagag cctttcgcaa acagggctgc aggtttttcc
     9001 ttgttaggtg cgcgctcacc atcactttga tagatattac ctcttttacg cataatgaca
     9061 gaaaggagcg catctgtttt tccggtttta ccgaaccagg tgagccccga gcctgtgctg
     9121 tggtcaccac tggcgatatt tccccacaga cttaaaccgt ttgtctctcc ggggggaagg
     9181 aaatctgctg catcggcagt tctgaagtcc acaacacctc ccagcccgcc actgccgtac
     9241 agactggagt ttggtccgcg gacaacttcg atacgtttga cgagtgccgg atccagataa
     9301 gtgccactgc tttttgccat gtcactgagt tgacggatgc cgtcaacaag aacaagtacc
     9361 ccgcttttgt catagccgcg tagattaaag gtctgcccat tggtccgtcc tgcaccagtc
     9421 tggctgagac cggcaacacc tttcagcatg ccggctgatg tgaccgcatt ctgactccat
     9481 ggcgtgtcat tcgtgacgac actgaccatt gacggcgttt caaataccgt gttctgattt
     9541 cctgtcgcga caacggtgat tgtatcttta gccatagaag aatgactggt ctctgcctgt
     9601 aaccctatcg ggagtatgat taatgtacaa atgacgtttc tgattacgtt catatacatg
     9661 cgaaaatata tgttaatatt gatatgttat aacatatcat tttattaatg caaccctgat
     9721 cacgcagcgt tcagtgataa ccagtgaata gcgactctaa tacccggtcg ctctgcaatg
     9781 tatcagcgcc tgcgaccgat gctatggata ttgatttttc taccgataaa gaaccagcaa
     9841 tacggaattt agactggccc cctgaatctc cagacaacca atatcactta aataagtgat
     9901 agtcttaata ctagttttta gactagtcat tggagaacag atgattgatg tcttaggacc
     9961 ggagaaacgc agacggcgta ctacacagga aaagatc
//
LOCUS       DAZFRE010000147         9917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFRE010000147 DAZFRE010000000
VERSION     DAZFRE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.148"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            370..1587
                     /gene="alaA"
                     /locus_tag="ACHKUI_004562"
     CDS             370..1587
                     /gene="alaA"
                     /locus_tag="ACHKUI_004562"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6600614.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHKUI_004563"
     CDS             1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHKUI_004563"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6600615.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2329..4161)
                     /locus_tag="ACHKUI_004564"
     CDS             complement(2329..4161)
                     /locus_tag="ACHKUI_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6600616.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHKUI_004565"
     CDS             complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHKUI_004565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6600617.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4909..5403)
                     /locus_tag="ACHKUI_004566"
     CDS             complement(4909..5403)
                     /locus_tag="ACHKUI_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6600618.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHKUI_004567"
     CDS             complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHKUI_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6600619.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYDVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6279..7481
                     /gene="ackA"
                     /locus_tag="ACHKUI_004568"
     CDS             6279..7481
                     /gene="ackA"
                     /locus_tag="ACHKUI_004568"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6600620.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7556..9700
                     /gene="pta"
                     /locus_tag="ACHKUI_004569"
     CDS             7556..9700
                     /gene="pta"
                     /locus_tag="ACHKUI_004569"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6600621.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
BASE COUNT         2590 a         2686 c         2386 g         2255 t
ORIGIN      
        1 gtttcacgcc ttgcccccat tgtgacacct cggaaatcat cacgctgtta aactctccgt
       61 aatgcggaaa tatcatcatc gcgattcagt agtgaattct taaacgaggc attttcacac
      121 aattatctta cagataaaaa aaccacactt acaattaaga atcagaataa ttcactacat
      181 aatattactt attaagcata tgcacctcat tattttgcca ttattaagtt attaacagca
      241 caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
      301 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
      361 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
      421 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
      481 ctgaacatcg gcaaccctgc cccgttcggt tttgacgcgc cagatgaaat tctcgttgac
      541 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaaggtct ttactccgcg
      601 cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat
      661 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
      721 agcggggacg aaatgttggt tcctgcacct gattacccac tatggaccgc agcggtttcg
      781 ctctccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
      841 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat tatcaaccca
      901 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
      961 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgac
     1021 gctgagcatc actccattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
     1081 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
     1141 aaaaaacacg ccaaaggtta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
     1201 gctaacgttc ctgcgcaaca cgccattcag accgcactgg gtggttatca gagcatcagt
     1261 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaattgatc
     1321 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa
     1381 atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga tttcctgttg
     1441 caggaaaaag tcctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
     1501 cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt
     1561 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
     1621 gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
     1681 gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg ctaatgcgca
     1741 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
     1801 tggcagctat caaaaatcgt aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
     1861 tggcgatgta ccacgatgct tcagaagtgc tcaccggaga tctccctact ccggtgaaat
     1921 acttcaattc gcaaatcgct caggaataca aggctattga aaaaatcgct cagcaaaaac
     1981 tggtcgatat ggttccggaa gagctgcagg atatctttgc gccgttaatt gacgagcatg
     2041 catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga
     2101 aatgtctgga agagctcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
     2161 aagcgacgct tgaagcgcgc cgcagccagg agatggacta cttcatggaa gtatttgttc
     2221 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag
     2281 tctgcgtcgc atcaggcaat aagcaccgga tgcgacatca ggctcttgtc aaaacggaaa
     2341 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa
     2401 cttaacaaag tcactgaagc tgtaattccc cggacctaaa accagtgtgt taaccggtga
     2461 agaaaccggc gtcataaagg cggcggatgc tgccatcgcc acgaccatcg caaatggata
     2521 aggcgacaca cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
     2581 cgcggtatta gaaataaata gcccaataac cgccgacaag acaaacaaac aacccagcat
     2641 catatgtggc ccataaccgc cgccaatgtc catcagccct ttcaccgcca gcgcgacacc
     2701 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
     2761 ccagtgaatg gatttatagg cactttcggc atcgatacag cggaatttcc ccatcagcag
     2821 gcaggcgatg atagcggcaa ctggattagg aatttcatcg gtcagcatta acgccaccat
     2881 tagcaccaga cagaaaatcg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
     2941 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag
     3001 tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
     3061 accttccagc gccacgccat tgcgctttag ccccactaca ttcaatccgt agcgggtacg
     3121 aaaaccaatt tcgcgcaccg atttaccaat aagttctgac tcaggaatta atgaaatctc
     3181 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
     3241 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
     3301 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
     3361 acgccgccag cgttcaacac cgatgacgtt agcaccataa cgttcacgta atttgagatc
     3421 gtccagtcgt tgaccaatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
     3481 ggtcagtcga tattcacgga taagatcgcg aaaggttcgg cgcgtccagc cttcgcgttg
     3541 tggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
     3601 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
     3661 acgcagcaat tcactgttga ctaccaggtt cggcggtgtc gccaccagcg tcatcatgcc
     3721 gcttatgagc ccggcaaaac ttaacggcat catcagacgc gacggcgacg tttgcatacg
     3781 catggcaacg cttaacacca cgggaataaa gatagcgaca acgccggttg aactcataaa
     3841 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
     3901 cgccactttg accagccatg tccccattac agtggcaaca ccggtacgga ccaaaccatc
     3961 gccaataata aacaaggcgg caatcaggac aacgttagga tcggaaaagc cggaaaatac
     4021 ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
     4081 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
     4141 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggataa cttgattctg
     4201 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
     4261 acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag gactaaatcg
     4321 acttcattca ggcgcggggt atctgccggg gcattaaccg caataacatg gcaacccgcc
     4381 gccagacctg aaagcacgcc agcgggagca tcttccacca caacacactc ctgcggcgca
     4441 agccccagca gctgcgcgcc taacagatac gcatcaggtt ctggttttcc gcgcttcacc
     4501 cgctcagcgg ttacaaacac ctctggcgcg ggaagccccg ctattttatg acgcgctcgc
     4561 gctaccggca tggagccaga agtcacaata gcccacggaa tacctgcttt attcaaatga
     4621 ttgagtaagg cgattgcccc cggaagcgcg gtaatacctt cggtttccgt ggcctcgatg
     4681 tgctccagac gcgtaaactc ggcggcaatg tctgcctcgg atttgcccgc cataaaatgg
     4741 cgcagagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgataac
     4801 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
     4861 gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttaat
     4921 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccagacatt
     4981 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccagc
     5041 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
     5101 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
     5161 gtcctgcaaa ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat
     5221 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccagttcac gcatctgtaa
     5281 tccataacca cgctcaatga ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
     5341 gagcatagtc atcatcttgt actggttaga caaaataaga cgttgcgcgt tggtcatttc
     5401 cattattgac tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc
     5461 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
     5521 tgaaggcacg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
     5581 ccaacacctg tccagactcc tgcaatttac cgcgaacgtc ataaaaccag ttgaggattg
     5641 caggaggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
     5701 ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccaga gcaatctgcc
     5761 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
     5821 taacgcgatt ttcgacaaag actggggcaa ggcgtttttc cagcggccac gtctttgagt
     5881 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
     5941 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
     6001 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
     6061 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
     6121 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
     6181 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
     6241 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
     6301 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
     6361 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
     6421 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
     6481 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
     6541 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
     6601 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
     6661 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
     6721 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
     6781 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
     6841 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
     6901 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
     6961 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
     7021 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
     7081 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
     7141 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
     7201 accgcctggc caaatacatc ggcgcctaca ctgcgctgat ggatggtcgt ctggacgctg
     7261 ttgtattcac cggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
     7321 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
     7381 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
     7441 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
     7501 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
     7561 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
     7621 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
     7681 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
     7741 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
     7801 tcagaaagat gtgctgatgg aagagatcat cgcgaactac cacgctaaca ccaaagacgc
     7861 tgaagtcgtt ctggtggaag gtctggtccc gacacgtaag caccagtttg cccagtctct
     7921 gaactacgaa atcgccaaaa cgctgaacgc agaaatcgtc ttcgttatgt ctcagggaac
     7981 tgatactccg gaacagttga aagagcgtat cgaactgact cgcaacagct tcggcggtgc
     8041 aaaaaacacc aatattaccg gcgttatcgt taacaaactg aacgctccgg ttgatgagca
     8101 gggtcgtacc cgtccggatc tgtccgagat ttttgacgac tccaccaaag caaaagtgaa
     8161 caacgttgat ccggcgaagc tgcaagaatc cagcccgctg ccagttctcg gcgctgtgcc
     8221 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
     8281 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
     8341 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
     8401 ccgccctgac gtgctggttg ccgcttgcct ggctgccatg aacggcgtag aaatcggtgc
     8461 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
     8521 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cttctcttag
     8581 cctgcagagc ttcaacctgg aagttccggt tgacgatcat gagcgtatcg aaaaagttca
     8641 ggaatacgtt gctaactaca tcaacgctga ctggatcgat tctctgactg ccacttctga
     8701 gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac ttgcgcgcaa
     8761 agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
     8821 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccag cagagatcaa
     8881 ccgtgttgca gcctctcagg gtgtagaact gggtgcaggc attgaaatcg ttgatccaga
     8941 agtggttcgc gaaaactatg ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac
     9001 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtt ctcggtacgc tgatgctgga
     9061 acaagatgaa gttgatggtc tggtttccgg tgctgttcac accaccgcaa acaccatccg
     9121 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
     9181 catgctgttg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
     9241 cgcagaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
     9301 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgctg gtagcgacgt
     9361 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgatc tgatgatcga
     9421 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcaccgaa
     9481 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
     9541 cacctacaaa gcggtacagc gttctgctga cctgatctct atcggaccga tgctgcaggg
     9601 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgatgata tcgtctacac
     9661 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
     9721 ttgcgctgcg gatatctgaa ccggaaatag tcactatttc cggttttttg tgcacagaaa
     9781 acccccagct aggctggggg ttccggaaag ctttcagctt tgagccagtt attaaaaccc
     9841 cttttgattt gttaaaacac cttgcggtct ggcaactgca agtgtcaaac aagaaatcaa
     9901 aagggggtcc caatggg
//
LOCUS       DAZFRE010000148         9834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFRE010000148 DAZFRE010000000
VERSION     DAZFRE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.149"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(53..1213)
                     /locus_tag="ACHKUI_004570"
     CDS             complement(53..1213)
                     /locus_tag="ACHKUI_004570"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6600622.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPVGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1219..1890)
                     /locus_tag="ACHKUI_004571"
     CDS             complement(1219..1890)
                     /locus_tag="ACHKUI_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6600623.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2038..3519)
                     /gene="tolC"
                     /locus_tag="ACHKUI_004572"
     CDS             complement(2038..3519)
                     /gene="tolC"
                     /locus_tag="ACHKUI_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6600624.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3724..4353
                     /gene="nudF"
                     /locus_tag="ACHKUI_004573"
     CDS             3724..4353
                     /gene="nudF"
                     /locus_tag="ACHKUI_004573"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6600625.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4354..4776
                     /locus_tag="ACHKUI_004574"
     CDS             4354..4776
                     /locus_tag="ACHKUI_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6600626.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4801..5628
                     /gene="cpdA"
                     /locus_tag="ACHKUI_004575"
     CDS             4801..5628
                     /gene="cpdA"
                     /locus_tag="ACHKUI_004575"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6600627.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            5628..6209
                     /gene="yqiA"
                     /locus_tag="ACHKUI_004576"
     CDS             5628..6209
                     /gene="yqiA"
                     /locus_tag="ACHKUI_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6600628.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6238..8130
                     /gene="parE"
                     /locus_tag="ACHKUI_004577"
     CDS             6238..8130
                     /gene="parE"
                     /locus_tag="ACHKUI_004577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6600629.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8175..9044)
                     /locus_tag="ACHKUI_004578"
     CDS             complement(8175..9044)
                     /locus_tag="ACHKUI_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO6600630.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGGFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9058..>9834)
                     /locus_tag="ACHKUI_004579"
     CDS             complement(9058..>9834)
                     /locus_tag="ACHKUI_004579"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO6600631.1"
                     /translation="IRSPKIYFILKGNIMNSYLKISLAGITMLSCLSPTLVCAEQPHG
                     FIDYRHEYLDDTRTHADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTG
                     LSVVYRFKPLDDKKFWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDK
                     ATDKSKGYGNDDRNNRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWHDGHKNYTASF
                     KVGYKMGSWIPYMSIADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2347 a         2397 c         2560 g         2530 t
ORIGIN      
        1 aatggtggat tataagagat tgccccgctt atcggggcaa tattaagctg tattactcaa
       61 caaaaatatg cggataaaac cgagacatat cctgggtgat caatgcacgg tcttcacgaa
      121 tgccaattcc gacaggttga tcgttcacca gccagctacc aatcagcata tagctgtcgc
      181 cgaatttcgg taacgggtgg aattgctgaa caatcatccc ttcttcgcca tacggacctt
      241 ccgctgctgc aatggttttg ccgttctcaa tgatcgacac gtttgcgcct tcacgggaga
      301 agatcggttt aaccacatat ttttccattt gcggatgatc atcttccgca aaataagcgg
      361 gcagcaggtt cgggtgattc gggaacatct cccacagtag cggtagaagc gctttgttgg
      421 agataatgct cttccacgcc ggttccagcc agcgtacgcc tgcatcctcc agcttggtgg
      481 agaacatctc acgcaacata aattcccacg gatacagctt gaacaggttg gaaatcacct
      541 gatcctgtaa atccgtgaac tgaccttttt cacctaaccc gatatcgtcg atgtagagga
      601 actcggtagc gatttcagct tccgtcgcgc agtcctgcaa atactgaatg gttccgcgat
      661 cttccaccgt gtcgcgacag caggtgagat gcagcaactg gaagccatac tgttcacgca
      721 gttcaacgaa gcgatcgatc agcttttctt gcaaactgtt aaactggtcg ctgccctccg
      781 gcaagttacc ggcgttaagc tgatcttcca gccagatcca ctgaaagaac gccgcctcgt
      841 atagtgacgt tggcgtatcg gcgttatttt ccagaagttt aggttcacca gtgccatccc
      901 atgccagatc aaggcgcgaa taaagcgatg gctggtgcgt cagccatgac tggcgcacaa
      961 aactccaggt gtgttttggt atgcggaatt tggtcatcag ctcatcgctg gcgatcactt
     1021 tttccaccac tttcaggcac atctggtgca gttcggcggt gacttcttcc agcttttcaa
     1081 cctgagagag ggtcaacttg tagtaagcat cttcacacca gtacggctcg ccgtacatgg
     1141 tgtgaaaatt gaaaccgtat tcgtgggctt tttcgcgcca gtccgggcgc tcggtaatac
     1201 tgactctttc catcggtatc agccacccat tgaacgagaa gaggtgccgg ttgcgctacg
     1261 ctgcatagtg ctttgtttgg caacagattc accaaaaccg ccacgggtaa cagtagttgt
     1321 ggtcgccggt tttggtgcca ttgccgtctt cggtacggtc atggtgcggc ctggctgggc
     1381 tgcgccatag tttttaccgg tcgcgtcggt atatttaccg taagccgggc tggccgggtt
     1441 tttcgaggag aacagcggct gctgtgcaaa tcccgcgccg ccgcccatca gacgccccat
     1501 catgtaaccg gccatcagcg gcatccagaa actcccgctg gattgctggg cctgcgcctg
     1561 gttttctggt gccatgccag cctgggctgg tgcctgctgg cactgacctt caccaaattc
     1621 agcaacacag tcttcacggg tggcgtattt cggcgcagta cgttcggctt ctttcagcgc
     1681 attgttgtac gcggtggtac attcggcgct tttgcctggg tttgcagctg aacagtcgtc
     1741 agcattttga tagagagaca ctgtttcatc actcttttca cagccagcca gcataaaaac
     1801 agtggcaacc gcgagagcga ctggtgtcag atggcgtgcg ctccagtttt tgcggaacga
     1861 tgcgtggcgt atggattttg tccgtttcat ttttgtcttc cgggaccagt ggtaaatacc
     1921 catcagaata gaggatggct ggtcgaaatt gaagcgagaa gggggaagaa tgcggcagat
     1981 aacccgtatc tttacgttgc cttacgttca gacggggccg aagccccgtc gtcgtcatca
     2041 gttacggaaa gggttatgac cgttactggt ggtagtgcgt gcggatgttt gctgaacgac
     2101 tggtgccggg ctatcaggcg cataaccatc agcaatagca ttctgttccg gcgtttgcgg
     2161 ggcaacgttt tccggattag tggaaaccgg tttgctcagc gcattgttca gtgccagcag
     2221 atcctgctcg ttcaacgtac ccagagctga cttaatattc aactggttaa tcaggtagtt
     2281 ataacgcgca tttgccagct cttgcttagc gttgtacagc gtggtggtcg catccaacac
     2341 atcaacaatg gtacgcgtac cgaccgagta acccgcttcc atcgcgtcta atgagctttg
     2401 agcggaaact acggcttgtt tgtaggcgtt aatgctactg atagaagcat taatgttgtt
     2461 gaaggaggaa cgtacggttt gcacgacgct acgatgcgcg ctttccagtt gctcgctggc
     2521 accaacaaag ttgtactggg cctgtttcac ctgcgagtta accattccgc cctgataaat
     2581 cggcagcgag aagctcaggc caactttgtt ctggcccata ttgctatcgt catactgggt
     2641 accagcggca ccacgggttt tcgaaccgct ataagaggtg tcagaaatcc cggtagaagc
     2701 cgttaaatcc agagtcggta agtgaccatc ctgcgcctgg cgaatttgct cgcgcgccag
     2761 gtcctggctc aagcgcgcct gtaacaacga caggttgcgt ttttcggctt ctttcagcag
     2821 cgtgttaacc ggctgtggtt tgtcggtttt aaagttttcg acattcagcg ccgccagttc
     2881 cggatagtag ttaccggtga tctggcgcag ctgctctacc gcgttatcaa ggttattacg
     2941 tgcggtcact tcgttcgcca gcacggtatc gtactgtgcg cgggcgttct gcacgtcggt
     3001 gatcgctacc aggcccacgt taaaacgttg ggtggtttga tctaattgac ggtagatcgc
     3061 ttctttttgc gcctgtgtat aggaaagaac gtcaatcgca ttcaacacgt tgaaataagc
     3121 ggtcgcggtg ttgaggatca aggtttgttg atcggtctga tacgtgacgt cctgaatccc
     3181 agctgctttt tcctgcagcg ttaacgcacg ccatttcgac atatcaaaaa tggattgagt
     3241 taactgcagg gacgcactgg tcgcgttaga gttgatgccg ttcgcgtcgc ggtagccgtt
     3301 gctataggtg taatctgcac ctaaacctag ctgtggcagt aatggactgc gcgcttcatt
     3361 aattttttca aaggcagcat cacgatcggc ggcagactta cgcaattccg ggttactaag
     3421 gcgtgcttgc tgataaactt gcatcaggtt ctcggcctgg ctcaacgaac tgaacccaga
     3481 gaggctcagg ccgataagaa tggggagcaa tttcttcatt tgcattcctt gtggtgaagc
     3541 agtatttagc gcgatcaaac tgtaaaatta ttgcggattc tagcagaagc cgctaccgca
     3601 aaaggttggc gttacgtgcc atagggcgtt aatgtgcctc aatttaacac atgactgctc
     3661 aaacggcagt caaacgtcgc tgaaattcac atttaattca ctattagtgc caggacatta
     3721 acaatgctta agccagacaa cctgcccgtt acatttggca aaaacgatgt agaaattatt
     3781 gcacgagaaa cactttatcg cggctttttt tcattagatc tttatagatt tcgtcatcgt
     3841 ctattcaacg ggcaaatgag tcatgaggta cggcgggaaa tttttgagcg cggtcacgcc
     3901 gcagtcttgc taccctttga cccagtacgt gatgaagttg tgctgattga gcagattcgg
     3961 attgccgcgt acgacaccag cgaaaccccc tggctactgg agatggttgc cgggatgatt
     4021 gaagagggtg aaagtgtgga agatgttgcc cgtcgcgaag cgattgaaga ggcgggactg
     4081 atagtcaaac ggaccaaacc ggtgttaagt ttcctggcaa gcccgggggg caccagtgag
     4141 cgttcgtcaa ttatggtggg cgaagtggac gccacgaccg caagcggtat tcacggtctg
     4201 gctgatgaaa acgaagatat tcgcgttcat gtggtaagcc gggaacaggc ataccagtgg
     4261 gtagaagagg ggaaaatcga caacgcagcg tcggtcatcg ctttgcaatg gctgcagctg
     4321 catcatcaag cgttaaaaaa tgagtgggca taaatgaagc gttacacacc tgactttcct
     4381 gaaatgatgc gcctgtgcga gatgaacttt tcacaattgc gccgtttgtt accgcgcaat
     4441 gacgcacccg gcgaaactgt aagctatcag gtggcaaacg cacaatatcg gctgacgatt
     4501 gtggaatcga cccgatacac taccctggtg acaatagaac agactgcgcc cgcgatcagt
     4561 tactggagcc ttccgtcaat gacggtgcgt ctgtatcatg acgcgatggt ggctgaagtg
     4621 tgttcaagcc agcagatttt tcgcttcaaa gcgcggtatg attatcctaa taaaaagttg
     4681 catcaacgcg acgaaaagca tcaaattaat cagtttttag cggactggtt gcgatactgt
     4741 ttagcacatg gagcgatggc gattccggtt tattagcgtc gtgaaaccta aggacaccat
     4801 ttggaaagcc tgttaaccct tcctctggct ggtgaggcca gagtcaggat tttacaaatt
     4861 accgacactc acctgtttgc acaaaagcac gaagccctgt taggggtaaa cacctgggaa
     4921 agctaccagg cggtgctgga ggcgattcgg ccacaccagc acgaattcga cctgattgtc
     4981 gcgacaggtg atttagcgca ggatcaatcc tctgcggcct atcagcattt cgctgaaggc
     5041 atcgcaagtt ttcgtgcgcc ctgcgtctgg ctgccgggca accacgattt ccagcccgcg
     5101 atgtacagtg cgttacagga tgcgggtatc tccccggcga agcgcgtgtt tattggtgag
     5161 caatggcaaa tcctgttgct ggatagccag gtgtttggcg tgccgcacgg tgagctgagc
     5221 gagtttcagc ttgagtggct ggaacgtaaa ctggccgatg cgccaggacg ccatacgttg
     5281 ctgctgctgc atcatcatcc gctacctgca ggttgtagtt ggcttgatca acacagcctg
     5341 cgtaacgcgg gcgaactgga taccgtgctg gcgaagtttc cgcacgtcaa atacttgctg
     5401 tgcggtcaca ttcatcagga gctggatctc gactggaacg gtcgccgctt gctggcaacg
     5461 ccgtcgacct gtgtgcagtt taagccgcac tgttctaact ttacgctgga caccatcgcg
     5521 cccggctggc gtactctcga gttacatgct gatggcacgc tgaccaccga ggtgcatcgc
     5581 ctggcggacg cacgcttcca acctgatacc gcttcagaag gctactgatg tctacgcttc
     5641 tttatttaca cggtttcaac agctcgccgc gctctgcaaa agcgagcttg ttaaaaaact
     5701 ggctggcgga acatcaccct gacgttgaga tgatcattcc gcagttgccc ccgtatcctt
     5761 ctgacgcggc agaactgctg gaatccattg tcctggaaca tggcggcgat tcgctgggta
     5821 ttgtcggttc gtcactgggg ggatattacg ccacctggtt gtcgcaatgt tttatgctgc
     5881 ccgcagtggt ggtcaacccg gcggtgcgcc cgtttgaact gctgacggac tatctcggtc
     5941 agaacgagaa cccctacacc gggcagcaat atgtgctaga gtcacgccat atttacgatc
     6001 tcaaagtcat gcagattgac ccgctggaag cgccggattt gatctggctg ctgcaacaga
     6061 cgggagatga agtgctggat taccgccagg cggtggcgta ctacgcttcc tgccgccaga
     6121 ctgtcataga aggcggcaac cacgcattca cgggcttcga agattatttc aacccgatcg
     6181 tcgattttct tggtctgcac catctctgac gatcaactcg aattgctaac ttaaaccatg
     6241 acgcaaactt ataacgctga tgccattgag gtactcaccg ggcttgagcc ggttcgccgc
     6301 cgtccgggga tgtataccga taccactcgc cctaaccatt tggggcaaga agttattgat
     6361 aacagtgtcg atgaagcact ggcgggccac gcaaaacgcg tggatgtaat cttacatgcc
     6421 gaccagtcgt tagaagtgat tgacgatggg cgcgggatgc cggtagatat tcacccggaa
     6481 gagggggtac cggcggttga actgattctt tgccgtctgc acgcgggcgg taaattctct
     6541 aacaaaaatt accagttctc tggcggcctg catggcgtgg ggatttcggt agttaacgcc
     6601 ctgtcgaagc gcgtagaagt taacgtacgc cgcgatggtc agatctataa catcgccttt
     6661 gaaaatggcg aaaaggtgca agatttacag gttgtcggca cttgcggtaa acgcaatacc
     6721 ggtaccagcg tgcacttctg gccggatgaa accttctttg acagcccgcg tttttctgtt
     6781 tcacgcctga cgcatgtgct gaaagccaaa gcggtactgt gcccgggcgt tgagatcact
     6841 tttaaagatg agatcaacaa caccgaacag cgctggtgct atcaggacgg tctgaatgat
     6901 tacctggcgg aagcggtaaa cggtttaccg acgctgccag aaaaaccgtt tatcggtaat
     6961 ttcgctggcg atactgaagc ggtggactgg gcgctactgt ggctgccgga aggcggtgaa
     7021 ctgctgaccg aaagctacgt caacctgatc ccaacgatgc agggcggtac ccatgttaat
     7081 ggcctgcgtc agggcctgct ggacgcgatg cgtgagttct gtgaataccg caacattctg
     7141 ccgcgtggtg taaagctgtc ggcggaagat atctgggatc gctgcgccta tgtgctgtca
     7201 gtaaaaatgc aggatccgca gtttgccggg cagaccaaag aacgtctctc ttcgcgtcag
     7261 tgtgcggcat tcgtttcggg cgtggtgaaa gatgccttca tcctgtggct gaaccagaac
     7321 gttcaggcgg cggagctgtt ggcagagatg gcgatttcca gcgcccagcg tcgtatgcgt
     7381 gcggctaaaa aagtggtgcg taaaaagcta accagcggcc cggcgctgcc tggtaaattg
     7441 gctgactgta ccgcgcagga tcttaaccgt accgaactat tccttgtgga aggtgactcc
     7501 gcaggcggat ctgccaagca ggcgcgcgat cgcgaatatc aggcgatcat gccactgaaa
     7561 ggtaagatcc ttaacacctg ggaagtctct tccgacgaag tgctggcttc gcaggaagtg
     7621 cacgatattt cggtagctat cggtatcgat cctgacagcg acgatttgag ccagcttcgt
     7681 tacggcaaga tctgtatcct ggcggatgct gactccgatg gtctgcacat tgccacgctg
     7741 ctctgcgctt tgtttgtaaa acacttccgc gcattggtga aacacggtca cgtttacgtt
     7801 gcactgccac cgctctaccg tattgacctc gggaaagagg tttattacgc gctgacggaa
     7861 gaagagaaag agggcgtact tgagcaatta aaacgcaaga aaggcaagcc aaacgtccag
     7921 cgttttaaag gtctcgggga aatgaacccg atgcaattgc gcgaaaccac gcttgatccg
     7981 aacactcgcc gtctggtgca gttgactatc gatgatgaag acgatcagcg tactgacgcg
     8041 atgatggata tgctgctggc gaagaaacgc tcggaagatc gccgcaactg gttgcaagaa
     8101 aaaggcgaca tggcggagat tgaggtctga taatcttaac ccggcaaaca tgtgttttgc
     8161 cgggttgtcg ttgattattt tattgggcac agtttattac gatctggaaa tagtgttgcc
     8221 agcgaaccag ccagagtttt ccagtccgta gccatttttt ctgcttgtgc agttgttatt
     8281 actgcacctt gttgtttaat aagctcatcc tccactctgg tatagtgaat aatggtaaaa
     8341 tccgtagagg accatttatc cttagagaac atcttcgtca tcgcatgaat gtttgcttgc
     8401 ccgtcatcga taaaaacaat cgcgtcatat ttgttcccca ttttatccag catataatcg
     8461 agaattacgc ctttatcttg cccggaagaa aaaaatcccc cctggctgta cagccatgac
     8521 cgtttgagtt tccctttttc aaagggtggc aaagtatcct ctttcttcct gagtgcggaa
     8581 gacgaaaaat caatgccgtt tcgctttaat tcccgcaatg tcggaaagag agtatccggt
     8641 gcacgggatg tcagagcaaa agcggtatgc ttttgttgcc actgttttaa cattgtcggt
     8701 aactgtggct cggtaagctg catagggcta agttcgtaaa gcatactgat tgtattttca
     8761 tacaggcaag gaactttttg cgagtcatcc ggctttaaag gcaatttatc ggtttgccat
     8821 tgataccaga tatcaccgcc aatgcgagtg ccactcgtta ataacgtgtt atcaatgtca
     8881 aaaaccaata atatcttatc ggatggatag agagtttcct tttctgtgat aattttactg
     8941 accgcactat attcgccagt aactatattt tttacctgtg cgtaagataa agcaggcaga
     9001 gcagagagca tcatccagag aatgatttta aatttattat tcatttttta ttccagatta
     9061 gaaagtataa gtcagacccc agcgccagcg gatttggcga ttacttgatg ttttatccac
     9121 acctttataa tccgcaatac tcatataggg tatccacgat cccattttgt accctacttt
     9181 aaatgatgcg gtatagtttt tatggccatc atgccaggtt aaataaccat tattgtaata
     9241 gaccaggttt aactgatatt ggaagttggt tttttgtggt cgatagtcga tccacacatc
     9301 gtaacggcgg ttattacgat catcgttgcc ataacctttg gatttatcgg tggctttatc
     9361 catgtcataa cgaaaccgac ccgaaacttt ccattctttg ctgaagtcat aacccgcaga
     9421 aagaccatat tcgtaagttg accaatattg cgtggtatca agccagaaca tgggttctaa
     9481 ccagaacttt ttgtcatcca gcggcttaaa gcggtaaaca acagataatc ctgttccgtt
     9541 attgccaaat tgtgaggggt cccacttatc tttatcacct tcatcggtgt tatagcgtaa
     9601 ttctcccatc aaaccgatac cattactaaa aaatgtacca aattcaacgc ggtccgcgtg
     9661 ggtgcgagta tcatcaagat actcatgacg ataatcaata aatccatgcg gctgttctgc
     9721 acagactagt gtaggtgaca ggcaggataa cattgttatt cctgcaagtg atattttaag
     9781 ataagaattc ataatattcc ctttcagaat aaaatatatt tttggtgatc gaat
//
LOCUS       DAZFRE010000149         9834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFRE010000149 DAZFRE010000000
VERSION     DAZFRE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.150"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            209..1366
                     /gene="acrE"
                     /locus_tag="ACHKUI_004580"
     CDS             209..1366
                     /gene="acrE"
                     /locus_tag="ACHKUI_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6600632.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSTKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1378..4482
                     /gene="acrF"
                     /locus_tag="ACHKUI_004581"
     CDS             1378..4482
                     /gene="acrF"
                     /locus_tag="ACHKUI_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO6600633.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4735..4956
                     /locus_tag="ACHKUI_004582"
     CDS             4735..4956
                     /locus_tag="ACHKUI_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600634.1"
                     /translation="MKRLIPVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5387..6412
                     /locus_tag="ACHKUI_004583"
     CDS             5387..6412
                     /locus_tag="ACHKUI_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6600635.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6480..7661
                     /gene="yhdX"
                     /locus_tag="ACHKUI_004584"
     CDS             6480..7661
                     /gene="yhdX"
                     /locus_tag="ACHKUI_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6600636.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALLWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7671..8774
                     /gene="yhdY"
                     /locus_tag="ACHKUI_004585"
     CDS             7671..8774
                     /gene="yhdY"
                     /locus_tag="ACHKUI_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6600637.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWVFLQANWVGSTRADCTKSGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRSRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8782..9540
                     /gene="yhdZ"
                     /locus_tag="ACHKUI_004586"
     CDS             8782..9540
                     /gene="yhdZ"
                     /locus_tag="ACHKUI_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6600638.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDDFFAHPKSERTRAFLSQVIH"
     gene            complement(9603..9718)
                     /gene="rrf"
                     /locus_tag="ACHKUI_004587"
     rRNA            complement(9603..9718)
                     /gene="rrf"
                     /locus_tag="ACHKUI_004587"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9758..9833)
                     /locus_tag="ACHKUI_004588"
     tRNA            complement(9758..9833)
                     /locus_tag="ACHKUI_004588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9798..9800),aa:Thr,seq:ggt)
BASE COUNT         2335 a         2357 c         2523 g         2619 t
ORIGIN      
        1 ttctatttct ttcattccgc gtcaaaacaa aacagtagaa tattaatctt tttttgtgtt
       61 tatgtgcctt gagttgccag tattcataac tattccttac atcgacgaat gataatttgt
      121 aggatagcga actgtatttt tctttctgcg agttaacgca ttgccttttt gggcaaataa
      181 cgcgcttttg gttttttgag gaatagtaat gacgaaacat gccaggtttt tcctcctgcc
      241 ctcctttatt ctgatctccg cggctttaat cgccggttgt aacgataagg gagaagagaa
      301 agctcacgtc ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc cgttagaagt
      361 taagactgaa ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc gcccacaggt
      421 tagcgggatc gtactgaatc gcaatttcac tgaaggcagc gatgtgcaag caggccagtc
      481 cctgtaccag atcgatcccg cgacctatca ggcaagttat gacagcacga aaggcgaact
      541 ggcgaaaagt gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt acgttccgct
      601 cgtgggtacg aaatacatca gtcagcagga gtacgaccag gccattgctg atgctcgcca
      661 ggccgatgcc gccgtgattg ccgcaaaagc cacagtcgaa agcgctcgca tcaatctcgc
      721 ttataccaaa gtgactgcgc caattagcgg acgtatcggc aaatcgaccg tgaccgaagg
      781 cgctcttgtc actaatgggc aaacgactga actggcgact gtccagcagc tcgatcctat
      841 ctacgttgat gtgacccaat ccagcaacga ttttatgagg ctgaagcaat ccgtagagca
      901 aggaaatttg cataaggaaa acgccaccag caacgtagag ttggtcatgg agaacggtca
      961 aacctatccc ctgaaaggta cgctgcaatt ctccgatgtg accgttgatg aaagcaccgg
     1021 ctccataacc ctacgtgctg tcttccctaa cccgcaacat acgcttttgc cgggtatgtt
     1081 tgtgcgcgca cggattgatg aaggcgtcca acctgacgcc attcttatcc cgcaacaagg
     1141 cgttagccgc acaccacgtg gtgatgcaac cgtgctgatt gttaacaata aaagtcaggt
     1201 tgaagcgcgc cctgtcgttg ccagtcaggc gattggcgat aaatggttga ttagcgaagg
     1261 tctgaaatct ggcgatcaag tcattgtcag cggcctgcaa aaagcgcgtc cgggagagca
     1321 ggttaaagcc actaccgata cccccgcaga tactgcatcg aagtaaggca atctgacatg
     1381 gcaaactttt ttattcgacg accgatattt gcatgggtgc tggccattat tctgatgatg
     1441 gcgggcgcac tggcgatcct acaattgccc gtcgcccagt atccaaccat tgcgccgcct
     1501 gcggtttctg tttcggcaaa ctatccgggt gctgatgcgc agaccgtgca ggatacagtg
     1561 acgcaggtta tcgaacagaa tatgaacggt atcgataacc tgatgtatat gtcctccacc
     1621 agcgattccg ccggtagcgt gacaattacc cttaccttcc agtccgggac cgatcctgat
     1681 atcgcgcaag tgcaggtgca gaacaaactc cagctcgcca cgccgttgct gccgcaggag
     1741 gttcagcagc aggggatcag tgttgaaaag tccagtagca gctatttgat ggtggcgggc
     1801 tttgtctctg ataacccagg caccacacag gacgatatct cggactatgt ggcctctaac
     1861 gttaaagata cgcttagccg tctgaatggc gtcggtgacg tacagctttt cggcgcacag
     1921 tatgcgatgc gtatctggct ggacgccgat ctgctaaaca aatataaact gacaccggtt
     1981 gatgtgatta accagttgaa ggtacagaac gatcagatcg ctgccggaca gttgggcgga
     2041 acgccagcgt taccagggca acagttgaac gcctcgatta ttgctcagac gcggttaaaa
     2101 aatccggaag aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc ggtggtacgc
     2161 ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact ataacgttat cgctcgtatc
     2221 aacggaaaac cggcggcggg cctggggatt aagctggcaa ccggcgcgaa tgctctcgat
     2281 accgcgaaag ccattaaggc aaaactggcg gaattacagc cattcttccc gcagggaatg
     2341 aaggttctct acccttatga caccacgcct ttcgtccagc tttctatcca ggaagtggtg
     2401 aaaacgctgt tcgaagccat tatcctggta ttcctggtga tgtatctgtt cttgcagaac
     2461 atgcgggcaa cattgatacc caccattgct gtacctgtcg tgttgttagg gacatttgcc
     2521 atactcgccg cttttggtta ctccatcaat acgctaacga tgtttgggat ggtacttgcc
     2581 atcggacttc ttgttgatga tgcgatagtc gttgtggaga acgtcgagcg cgtaatgatg
     2641 gaggataaac tcccccccag agaagcgacg gaaaaatcga tgtcgcaaat ccagggcgcg
     2701 ctggtaggta tcgcgatggt actgtcggcg gtatttattc cgatggcatt cttcggtggc
     2761 tcaacagggg caatttatcg acagttctct atcactattg tttcagcaat ggcgctttca
     2821 gtactggttg cgctgattct cacccccgcg ttatgtgcaa cattgctgaa accagtctct
     2881 gccgagcatc acgaaaataa gggaggattc ttcggctggt ttaacaccac tttcgatcat
     2941 agcgttaatc actacaccaa tagcgttggc aaaatcctcg gttccacagg gcgatattta
     3001 ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt ttttacgtct tccgtcttcc
     3061 ttcttacctg aagaggatca gggtgtcttt ctgaccatga ttcagttacc cgctggcgcg
     3121 acgcaagagc ggacgcaaaa agtgttggat caagtgacgg attactatct gaagaacgag
     3181 aaagcgaacg ttgaaagtgt ctttacagtt aacggcttta gcttcagcgg ccaggcacaa
     3241 aacgccggta tggccttcgt cagtctgaaa ccgtgggaag agcgtagtgg tgacgaaaac
     3301 agtgcggaag cagtaatcca tcgtgcaaaa atggaattgg gcaagatccg cgacggtttt
     3361 gtcattccat ttaatatgcc agccattgtt gaactaggca cggcaacggg tttcgacttt
     3421 gagttaattg atcaggctgg gctgggtcac gatgccctga cccaggcccg taaccagttg
     3481 cttggcatgg cggcgcaaca tcctgccagc ttagtcagcg tgcgccctaa tggcctggaa
     3541 gacaccgcgc agtttaaact ggaagttgac caggaaaagg cgcaggcatt aggtgtttca
     3601 ctttctgaca tcaatcagac catttcaacg gcgttgggtg ggacttacgt taacgacttc
     3661 atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg atgccaaatt ccgtatgctg
     3721 ccagaagatg tcgataaact ttatgtccgc agcgccaacg gcgaaatggt gccattctct
     3781 gcctttacca cttcacattg ggtgtatggc tctccgcgac tggaacgcta caacggtctg
     3841 ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca gttccggcga tgctatggcg
     3901 ttgatggaaa accttgcgtc aaaattaccg gcgggcattg gttatgactg gacgggtatg
     3961 tcatatcagg aacgtttatc gggaaaccag gctcccgctc tggtagcaat ttcctttgtg
     4021 gttgttttcc tgtgccttgc tgcactctat gaaagctggt caattcctgt ctcggttatg
     4081 ttggtcgtgc cgttagggat tgtcggcgtg ctactggcgg cgacactctt taatcaaaaa
     4141 aatgacgtct actttatggt gggcttgcta acgacaattg gcttgtcggc taaaaacgct
     4201 attttgattg ttgagttcgc taaagatctc atggagaaag agggtaaagg tgttgttgaa
     4261 gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc tgatgacctc tctcgccttt
     4321 attctcggcg tattaccgct ggctatcagt aacggggccg gcagtggcgc gcaaaacgca
     4381 gtcggtatcg gggtaatggg cggaatggtc tctgcaacgt tgctggcaat cttcttcgtg
     4441 ccagtgttct ttgtggtgat ccgtcgatgc tttaaaggat aaatcagaaa cataaaggcg
     4501 ctttcaggcg ccttttttat tttcagtgaa ccccataaaa attaataaga tattcttctt
     4561 ctcactcttt aaaaactttc tatagttccc gctcccttca ctatttttac aattcacata
     4621 aattgtcatt attcattccg caagatttat aacctccagt gataaaatgg cattcagctc
     4681 gttaataaga gagttaactt attaagcgtt agcgtttatt actgaggtaa caccatgaaa
     4741 agattaattc ctgtgacatt gctcaccgca ttgctggcgg gctgtgctca cgattcgccc
     4801 tgcgttccgg tttatgacga tcagggtcgt ctggttcaca ccaatacctg tatgaaaggc
     4861 acgactcagg ataactggga aacggctggg gctattgccg gcggggcagc agctgttgct
     4921 ggcctgacga tggggatcat cgctctgtcg aaataactaa acagggcgcg gagctccgcg
     4981 ctctttttaa cgatacagta tctttccata agataaaata ttgctatatt ctgctgggta
     5041 attccctgct gatttagcat gttatatcac gttatttcgc tgctttcgaa ataacgcatc
     5101 aatttaactt tcacacatct caccagaaat atactcacgc taacaatcat tattctgctt
     5161 agtaaaaaac gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt ttcgcaccat
     5221 tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac gcgaaatcgt
     5281 gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg taaaagacgt
     5341 ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga aaaagatgat
     5401 gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg cgcatgctgg
     5461 cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca gtgatggatt
     5521 acctgggttc tcttatgccg atgctgacgg taagttttca ggtattgatg tagatgtttg
     5581 tcgtggtgtt gccgctgctg tattcggtga cgatacgaaa gtgaaatata ccccgctcac
     5641 tgcaaaagaa cgcttcaccg ctttacagtc aggggaggtg gatttgctct cccgtaatac
     5701 gacctggact tcatctcgcg atgccgggat gggaatggca tttactggcg tcacttatta
     5761 cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga aagaactgga
     5821 tggcgctacc gtctgtattc aggcggggac tgataccgaa ctcaacgtcg ccgattactt
     5881 caaggcaaat aatatgaagt acacgccagt aaccttcgat cgctctgacg aatcagcgaa
     5941 ggcactggaa tctggtcgct gcgatacgct ggcctcggat caatcacaac tgtatgccct
     6001 gcgcatcaaa ttaagcaacc cggctgaatg gattgtctta ccggaagtta tctccaaaga
     6061 accgcttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg tacgctggac
     6121 gcttttcgcc atgctgaatg ctgaagagat gggcatcaat tcccagaacg tcgatgaaaa
     6181 agcggctaat ccggcaacgc ctgatatggc acatctgctg ggtaaagaag gcgattacgg
     6241 caaggatctg aagctggata ataaatgggc ttacaacatc atcaaacagg tgggtaacta
     6301 ctcggaaatt tttgagcgta acgtaggttc agaaagcccg ctgaaaatta aacgtgggca
     6361 aaataatctc tggaataacg gcggaattca gtacgcgccg cccgtgcgtt aagtcggtgt
     6421 gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc gaggtttcta
     6481 tgtctcatcg ccgctcaacc gttaaaggct cactctcctt tgccaaccct acggttcgcg
     6541 cctggttatt ccagatcctt gccgttgttg ctgttgttgg cattgttggt tggctatttc
     6601 acaacactgt gacgaatctc agtaatcgtg gcattacttc aggttttgcc tttctggatc
     6661 gcggcgctgg cttcggtatt gtccagcatt tgatcgatta ccagcagggt gatacctacg
     6721 gacgcgtttt cattgttggc ttactcaata cgctactggt ttctgcattg tgtattgtat
     6781 tcgcttctgt tctgggcttc tttatcggtc tggcgcgact ttcggataac tggctgctac
     6841 gaaagctttc cacaatttat attgagatct tccgtaatat tccgccattg ctgcaaatct
     6901 tcttctggta ctttgccgta ttgcgcaatt tgcccggacc acgacaggca gtgagcgcgt
     6961 ttgatctcgc ctttttgagc aatcgtgggc tttatattcc gtcgccgcag ctgggagacg
     7021 gatttattgc gtttatcctc gctgttgtta tggctatagt cctttcagtt gggctattcc
     7081 gctttaataa aacataccag ataaagaccg gacaactgcg ccgcacctgg ccattcgccg
     7141 ctgtgttgat cattggtttg cctttactgg cgcaatggct ttttggcgca gcactactct
     7201 gggatgtccc agcccttcga ggctttaatt tccgcggcgg gatggtttta attcctgaac
     7261 tggcagcctt aacgctggcg ctttcggttt atacttctgc atttatcgcc gaaattatcc
     7321 gcgcagggat ccaggcagtg ccttatggtc aacatgaagc agctcggtca ctgggattac
     7381 ccaacccggt cacgctacgc caggtcatta ttccccaggc attgcgggtg attattccac
     7441 cgttaaccag ccagtatctc aacatcgtca agaactcctc tcttgccgcc gctattggct
     7501 atcccgatat ggtttcgctg tttgctggca ccgtgctgaa ccaaacgggg caagccatcg
     7561 agacgatagc catgaccatg tcggtctatc tgattatcag cctgactatc tcgctgctga
     7621 tgaatatcta taaccgccgc atcgcgatcg ttgaacgcta aggagtcatg atgacaaaag
     7681 tattgctgtc tcatcccccg cgcccggcga gccataactc aagccgcgcg atggtgtggg
     7741 tgcgaaaaaa tctgttctcc agctggagca atagcctgct gactattggc tgcatatggt
     7801 tgatgtggga actgatccca ccgttgctga actgggtatt tttgcaggct aactgggttg
     7861 gctcaacgcg tgccgactgc acaaaatccg gtgcctgttg ggtcttcatc cacgaacgat
     7921 ttggtcagtt tatgtatgga ctttacccac acgaccaacg ctggcgaatt aacctagcat
     7981 tactgattgg ccttgtttct atcgcaccaa tgttctggaa aatacttccg catcgcagtc
     8041 gctatattgc ggtctgggcg gtgatttacc cactggttgt ctggtggctg atgtatggtg
     8101 ggtttcttgg tcttgatcgg gttgaaaccc ggcaatgggg cgggttgacg ctaactttaa
     8161 ttattgcatc agttgggatt gcgggggcgc taccgtgggg gatcttactg gcgttaggtc
     8221 gccgctccca tatgccgatt gtgcgtatct tatcggtcat ttttatcgag ttctggcgcg
     8281 gcgtaccgct gattaccgta ttgtttatgt cttcggtcat gctgccgttg tttatggcag
     8341 aaggcaccag tatcgacaaa ctgatccgcg cgctggttgg cgtgatcctg tttcagtcag
     8401 catatgttgc ggaagtcgtg cgaggcggat tgcaggcgct gcctaaaggg caatatgaag
     8461 cggcagagtc gctggcattg ggttactgga aaactcaggg gctggttatt ctgccacagg
     8521 cgttgaagct ggtaattcct gggctggtaa ataccatcat cgcactcttc aaagatacca
     8581 gtctggtgat catcatcggg ttgttcgatc ttttcagtag cgttcagcag gcaaccgttg
     8641 atcccgcctg gttgggtatg tcgacggaag ggtatgtttt cgccgcgctg atctactgga
     8701 tcttctgttt cagcatgtca cgttatagcc aacatctgga aaaacgtttt aacaccgggc
     8761 gtacaccgca ttgaggacac tatgagccaa attttactgc aacctgctaa cgcgatgatt
     8821 acgctggaaa acgtcaataa atggtatgga caattccatg ttttgaaaaa tataaattta
     8881 accgtgcaac cgggcgaacg gatcgttcta tgtggccctt caggttccgg taaatcgaca
     8941 accattcgtt gtattaatca tctggaagaa catcaacagg gacggatcgt ggtagatggc
     9001 atcgaactta atgaagatat ccgcaatatt gagcgcgtca ggcaggaagt gggaatggtc
     9061 tttcagcatt tcaatctctt ccctcatctg accgtgttac agaactgtac cctggcaccg
     9121 atttgggtac gcaagatgcc aaagaaagag gctgaagctc tggcgatgca ttaccttgag
     9181 cgggtgagaa ttgccgaaca tgcccagaag tttcccggac agatttctgg tggtcagcag
     9241 caacgtgttg ccatagcacg ttcgctgtgt atgaagccga aaattatgtt gtttgatgag
     9301 ccaacgtcgg cgctcgatcc tgagatggtg aaagaggtgc tggatacgat gattgggctg
     9361 gcgcagtcgg gtatgacaat gttgtgtgta acacatgaga tggggtttgc acgaaccgtc
     9421 gctgaccggg tgatttttat ggatcgtggg gaaatagtgg agcaagctgc acctgatgac
     9481 ttttttgcgc atcctaaatc agagcgtacg agggcatttt tatcgcaggt aatccattaa
     9541 ttgaatgtta gttcgaaaag caaaaaggcc atcctttcgg atggcctttt gcttgatttg
     9601 atgtctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc
     9661 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccagaca
     9721 aattcttttc taatctgccg aactttaacc taaaaagtgg tgctgatacc cagagtcgaa
     9781 ctggggacct cacccttacc aagggtgcgc tctaccaact gagccatatc agca
//
LOCUS       DAZFRE010000150         9631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFRE010000150 DAZFRE010000000
VERSION     DAZFRE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.151"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..228)
                     /locus_tag="ACHKUI_004589"
     CDS             complement(<1..228)
                     /locus_tag="ACHKUI_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFO6600639.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDASRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISG"
     gene            complement(225..563)
                     /locus_tag="ACHKUI_004590"
     CDS             complement(225..563)
                     /locus_tag="ACHKUI_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6600640.1"
                     /translation="MQAGRLRDRVTILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     RERISGGAETAQATVRVWMRFRRDVTAASRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            complement(572..889)
                     /locus_tag="ACHKUI_004591"
     CDS             complement(572..889)
                     /locus_tag="ACHKUI_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6600641.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(934..2160)
                     /locus_tag="ACHKUI_004592"
     CDS             complement(934..2160)
                     /locus_tag="ACHKUI_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6600642.1"
                     /translation="MAVDIKDVEQVAQDLQQKFDDFKAKNDKRIDAIEQEKGKLAGEV
                     ENLNEKLTELENLKSDLEAELAEVKRPAGGTQNKVASEHKEAFIGFMRKGREDGLREL
                     ERKALQVGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGT
                     TSGWVGETDARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALE
                     FAEQEEIAFTSGDGSKKPKGFLVYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKL
                     IYTLRKAHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLMKIGAATRQKAAA"
     gene            complement(2174..2824)
                     /locus_tag="ACHKUI_004593"
     CDS             complement(2174..2824)
                     /locus_tag="ACHKUI_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6600643.1"
                     /translation="MQTKQRLDIPLNLKSVSDSGEFEGYGSVFGVKDSHDDVVVPGAF
                     TTTLQNWSEKKALPALLWQHRIDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYILKDWEYDREKGVFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPDQKIIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVDAALNALKNLKF"
     gene            complement(2802..4043)
                     /locus_tag="ACHKUI_004594"
     CDS             complement(2802..4043)
                     /locus_tag="ACHKUI_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6600644.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELAEAIGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVDPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGRFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTS
                     PSAGDDNGKKKESGDADKTAS"
     gene            complement(4043..4225)
                     /locus_tag="ACHKUI_004595"
     CDS             complement(4043..4225)
                     /locus_tag="ACHKUI_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600645.1"
                     /translation="MFLIILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     AARYLDRTRQSVGGGK"
     gene            complement(4237..5994)
                     /locus_tag="ACHKUI_004596"
     CDS             complement(4237..5994)
                     /locus_tag="ACHKUI_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO6600646.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(5994..6476)
                     /locus_tag="ACHKUI_004597"
     CDS             complement(5994..6476)
                     /locus_tag="ACHKUI_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6600647.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDSKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVMIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(6623..6973)
                     /locus_tag="ACHKUI_004598"
     CDS             complement(6623..6973)
                     /locus_tag="ACHKUI_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6600648.1"
                     /translation="MPSQIPRACRKRGCAGTTTDSSGYCDKHRSEGWVQHQRGLSRHQ
                     RGYGLKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDTDSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(7016..7171)
                     /locus_tag="ACHKUI_004599"
     CDS             complement(7016..7171)
                     /locus_tag="ACHKUI_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600649.1"
                     /translation="MPRTTAKWALVAILVPFLLAGCVSLDKARQLFDTASQVCEIVDS
                     VRQCMQN"
     gene            complement(7213..7590)
                     /locus_tag="ACHKUI_004600"
     CDS             complement(7213..7590)
                     /locus_tag="ACHKUI_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO6600650.1"
                     /translation="MSGKFRFSRRSEKNLVGVKPQLVAVVRRALELTEVDFGITEGLR
                     TKERQKQLVAEGKSQTMNSRHLTGDAVDVVAWVGSQVSWDWPLYEKIAQAFKQAAAEL
                     GTAIEWGGDWKTLKDGPHFQLKR"
     gene            complement(7593..7868)
                     /locus_tag="ACHKUI_004601"
     CDS             complement(7593..7868)
                     /locus_tag="ACHKUI_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6600651.1"
                     /translation="MLSNLPGLLNVALCTVIVLTLFFYRRRDSRHKPLMSWLAWLLML
                     LYAFAPLSYLCGRPLAANWLEVFFNLLFCVLVVRARGNVTKIFPLLR"
     gene            complement(7858..8250)
                     /locus_tag="ACHKUI_004602"
     CDS             complement(7858..8250)
                     /locus_tag="ACHKUI_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO6600652.1"
                     /translation="MSEPLSGSGTAAALGGATVFGLFTGTDFGIVFGAFAGALFVATM
                     PQALSAWRVAAHFLVSFIVGVLGAHVLSAWIASKTGYDGTSADALCAVLVAVVSVKIL
                     SFIHQQDIASLVSGLFSRLRGGGGGNVK"
     gene            complement(8338..8490)
                     /locus_tag="ACHKUI_004603"
     CDS             complement(8338..8490)
                     /locus_tag="ACHKUI_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6600653.1"
                     /translation="MKSARLVLAAILLFLVVAVDFTGRLMSVLADGVLVAMALAVLWP
                     LLRKSE"
     gene            complement(8487..8912)
                     /locus_tag="ACHKUI_004604"
     CDS             complement(8487..8912)
                     /locus_tag="ACHKUI_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6600654.1"
                     /translation="MFGIFKKKTRKAITEVKKMENRDAVEATVWGAYSIAFADDTCDA
                     KEIAVLEKTIAALPAFAPFSGEIAQMSANIRARYEASPRSANAQALRELADVAGTAEA
                     VDVLCLCLDIADQDGIGPDEEAQLKKIAQALQLPLEQYL"
     gene            complement(9486..9560)
                     /locus_tag="ACHKUI_004605"
     tRNA            complement(9486..9560)
                     /locus_tag="ACHKUI_004605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9525..9527),aa:Gly,seq:tcc)
     gene            complement(9564..9631)
                     /locus_tag="ACHKUI_004606"
     tRNA            complement(9564..9631)
                     /locus_tag="ACHKUI_004606"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9604..9606),aa:Thr,seq:tgt)
BASE COUNT         2251 a         2874 c         2225 g         2281 t
ORIGIN      
        1 acccgagata atttcccccc gacgctttga acgctgcgtg agaacaacca catttttctt
       61 cagtttcccg gttcgctccg gcgcacgttc aacaactgca tcccgcataa cttcagcacc
      121 ggcacggctg gcatcgcgca gaaccttatt gttttctgcc ctgctgagcg tctccagatc
      181 ccgtgcaata tccgccagac cggaaaaatc aagactgaaa tccatcacac attccccttc
      241 aggctgcaga gtatttcaag ccgggtggcg cgtgcatccg gtattggtgg cccttctata
      301 ccaagaatgg ctcctttgaa tgcaccggtc agcactttca gacgtgaagc tgccgtcaca
      361 tcgcgtcgga atctcatcca gactctgacc gtagcctgag cggtttctgc acctcctgat
      421 attctctccc tcccgctgat acccttaact tctgcccata cggtagctcc ctccgtcacc
      481 gtctccaccg gatgccctga cggagagcgg gcggtggtga cattcagaat agttacgcga
      541 tcacgtaatc tgcccgcctg catggttcct cctacaaagg aataaaacga taaggctcca
      601 gcagagaaga aaaaccaaac gggactggtg ccttgctgac atctgaggaa ttttcccggt
      661 tttcgtacca gtgtccgacc agcaacatga gcgccaacaa aacatcatca gctataagca
      721 ccccttcagg atcaccttcc ggcaccgtct cctcataaag cttacggttg ataaaatttt
      781 ctgccttgcg gcaggcagcc cggaaataca gcatcagtaa ctcatcatca gttgcatcat
      841 ctgtatcaat acggcactgc gccctgagtt tttccactat tgctgccatc agaaactcct
      901 gcccacaaca ctgtgtgggc ataaaaaact gcattacgca gcggctttct ggcgggttgc
      961 agccccaatt ttcatcagct taatcgcctg agaatccacc agcataccgc cggttcgctt
     1021 ggtggtataa aaacccacaa acggtttatt ggtgtacgga tcgcgcagga tacgggtacc
     1081 gatgcgatca acgatggtat agccacgttt gaagttacca aacgcaatgg cttttgcatc
     1141 ggcagcaata tccggcatct gttcattctc aacgatgcca taccctgcca gagaagaagg
     1201 ctgacccagc tcaatacccg gacgccacag ataatttccg tcgttatcct tcagcagacg
     1261 aatggcaaac aggctgctgt tgttcatcat gaacttcgcg ccgctgcgat gcgccttgcg
     1321 cagggtgtaa atcagtttaa tgatcgcatc ggcagtcacg ccggaagccg caccggaagc
     1381 aatgtgctga agtttgccaa acgcacgggt cttgtcatct tcatcggtgg actcataaac
     1441 cagaaaacct tttggttttt tgctgccgtc gccactggta aaggcaattt cttcctgttc
     1501 ggcaaattcc agcgccagct cactgttgat ccagtcttcc acattgaaga aagcatcatc
     1561 gagcattttc tgggtggcct gcgggttgcc gtagatttcc cccataaagg gttcaatcag
     1621 ccccagtttt gaggtggcgg tttccggacg tgcatccgtt tcccccaccc atccggacgt
     1681 tgtgccgccc agattcacca gttttttata atccgagcca ccgagggtga tcacggtggc
     1741 ttcctggcgc atcaccacct catctttcag cagcgtcaga atggtgcgat ccagttcttc
     1801 cggaatggca taaccaccat cttcatcatt gcccacctgc agcgccttac gctcaagctc
     1861 acgcagaccg tcttcacgcc ccttgcgcat aaatccgata aacgcttctt tgtgttcact
     1921 ggcaacttta ttttgcgtgc cgcctgccgg acgcttaact tcagccagct cagcctcaag
     1981 atcacttttc aggttttcca gttcagtcag tttttcattg aggttttcca cttctccggc
     2041 aagttttccc ttttcctgct cgatcgcgtc gatacgtttg tcgttttttg ccttaaaatc
     2101 gtcaaacttc tgctgcaaat cctgcgcgac ctgttccacg tctttaatat caacagccat
     2161 tatttactcc tgattaaaat ttaagatttt tcagtgcatt cagtgcggca tccacatcct
     2221 cagcatcacg cagggataaa gcgccatatc ccccggccat gaatgctttg gcctgggttc
     2281 gcgagagtcc gacatcgcgc aggacccgct caataatttt ctgatcgggg atctccccac
     2341 gcgccagcgc atttttcaca tcgctgatgc gtgcttcatc attggaagga aacgtcacca
     2401 gactgacctc ccacaggtcg atctctttca gcaggaatac ccctttttcc cggtcgtact
     2461 cccagtcttt caggatgtag ccaatagaaa ggccggttaa agaaccggcc ttcatatggg
     2521 catgtgcacg ttttgccagg ggatcatcat caatgagtaa tcgtcccctg acataaagcc
     2581 cgacatcatc ttctttcatt tcggtgtaca caccgatggg ctcatctatg cggtgctgcc
     2641 agagcaacgc aggcagcgcc tttttttcgc tccaattctg gagtgttgtg gtaaaggctc
     2701 cggggaccac cacatcatcg tggctgtcct taacaccaaa aacagaaccg taaccttcaa
     2761 attccccgga atcactgacg gatttcaggt tcagcggtat atcaagacgc tgttttgtct
     2821 gcatctccac tctccttttt cttaccgttg tcatcgccag cagagggact ggtggtcatg
     2881 ttcatcggtg tcagatacac atcaccaccc ggtcggggat tcatatcctc cagatcacgg
     2941 cagtcattag gggaataaat cccccagttg atccccgtgg catacgcttc aaagcgggat
     3001 ttcatgtcgc cacgcaacaa tgctccggca ttaaatttgg cgtaaaacct cccctgcttg
     3061 ctctccctga ccagccctgt attgatccgc tgctcaatac gggtcagata cggcacaagg
     3121 gaatagttaa tgaaaccaag ccccagctct tcaatattgt tgaaggtggc gcgatcggtg
     3181 ttctgcacca tatgcagcgg cacgcggaac aggcgacaga tttcttccag ctgaaacttg
     3241 cgggtttcca ggaactgact gtcctcggcg ttcagcgcca tcgacttcca gtccagcccc
     3301 atttccagaa tcatcgggcg gtgagcattg ccaagccccg tgtgacgctc ctcaaaatct
     3361 ttcttcagac gctcgtaagc atccggcgtg agcttttgtt ccgtacgcaa cacaccggat
     3421 gtcaccgcac cattaccaaa caacctggca ccgtgctcct cggttgccgc tgccagtgaa
     3481 atggcctcgc gcgcatatgc aatgggattc agaccgacaa gtccatccag cgtcagggtg
     3541 cgcacatgcc agatttcatc ctgggtcagc acatccacgg aaccatccgg aaacgtcacc
     3601 tgataaaccg gctgccactg gctgttcagc ttcgggtcca cacagcccgg atctatcggg
     3661 agaagctcca ccacttcccc cagtgccttt accttgtagg cgtaaaaatt cccccgcaga
     3721 cacaggcaga caatgaccag ttcccagaat tcctgcggcg tcatgtagcc attgggtttt
     3781 gccgaaatca gcttatgcag ccgttcatcc accgcccgtg ttttaagggt gccgctgatt
     3841 ttgtagagac tgcagggcag cataccaaca gactcagcca gcaccctgac gcaggaatac
     3901 accgccgtca gccgcatggc ccgctggctg ctgatccgct ttccggtata ggtgtcgtat
     3961 gacagcccga tagcctccgc cagctctgct ggcgtggtca ctggtgcgtc actttttcgt
     4021 tgaaataacc ccgaaaagaa cactatttac ctccgccgac agactgccgt gtacggtcga
     4081 gatatcgtgc cgccagccac gaccagaaca ggcacagcgc cccggcaaca acaaaacccg
     4141 ccgggggata aatcagccag gcaccatacg ccagcaaaag cgcacccagc acgcccacca
     4201 gtggcgcgag aattatcaga aacataatga cctcggttaa agcgagcgga tgcccacgct
     4261 gaccagatgt tcagacagat ccggctccgg ttcaccacca ttgaccagca tccggctcat
     4321 tgctgtaaac atcgcaacag ggccgtcgat tttggcttcc ggcgtggatt tattcgggaa
     4381 gatattgtcg tttttgtccg gttttaccgt aacgttagac atcatccagt tcatgaccgg
     4441 atgattgctg tgatggaaac gcccggcata gaccagtgat tccgtttcct tcatggcctc
     4501 tgacagattg cgaaccgtct gcggaacctc caccagcggt atcccttctt cagccagtgc
     4561 caggctgaac tgcatcgcgc tccacgggtc aaatcccagt tccctgaggt tttcaccacc
     4621 aatccattcc agtaagtcac tttttatctg agcatgatcg ataacatcac catccgtcag
     4681 gatgagctta tccatctccg cccatttccg gtaaagttct gcctgctgcc gcgagcaccg
     4741 ctccagccgt ccttccggga gccagaattt aaaatcagca tgaacatgtc cgttatcggt
     4801 tcgccagagt tttgccgccg cacagatatc aatcttatga gcaaggtcga cgccgaccca
     4861 catgggatac gttttcagct catgtcgtgg ggcaatatat tcgcatttct cccacttaat
     4921 catgtccatc caggcagact cggcagtgac ccacacattc atatgtttcg tgaaaaaatt
     4981 cacccgcgct gaaacctgtt ccttcgcttt tttcgccaga cgacgcagat catcccagcg
     5041 tttacagatg cccaggcccg gattcgcttt ctgccagacc gtttcatcaa acggatcatc
     5101 tccctcatcg agcgtgtaaa taatcgcaaa gtaggagtcg tcttttaccg cgccctccac
     5161 gtcgctgtta tagcctcgca ataccttgat ggcgtaatcg cgttgctcgt aacaaatccc
     5221 ttccttgtta aagcccgccg tggtgatacc aaataacagg gactgcagac gggcaccggt
     5281 tgccgtttcc agaacgtccc acacgtcgcg ggttttatgt gcatgcagct catcaataat
     5341 ggcgcagtgg atgttcagac cgtccaggtt gtttgcatcc gaggaaagcg gttcaaattt
     5401 tgatgcgctc tgctcctggt aaatcgccag cttgttgaaa tcaaacaacc gcccgagtgt
     5461 cgaacgggct tttctgacca tatttttggc gtcttcaaac acgattctgg cctggtcacg
     5521 cgtggttgcg gctgaataca cctcagcccc gccttcacca tcagccccgg tcatatacag
     5581 cccgataccc gatgacaggg ttgattttgc gtttttacgg gcgacttcgt tgtatgccgt
     5641 ccggaaccgg cgcaccatca ccgggcgtcc gctgccatcg ctgcgcatga caacttcccc
     5701 ggtctcttca ttgaccagcg gaatgacaaa accaaaaata ttaatgagga taaatacatg
     5761 ccagtccatc aactcaatgg gctggcctgc cagcgcccct ttcacatggg gcacaaattt
     5821 gtagaaattc aggatgtgct gcgcacgggg ttcactgaaa taaatccccc gctcttcgcc
     5881 gtacttcaga tcatcaagaa aacgctggca ggccagacgg acaaattcgc cagcgacaat
     5941 ttctcctgca acaacacgtt cggcgtagcg gatcccgtca gccacttttg ccatcagtct
     6001 ctcgctttta aaagctctgc caacggatca acatcatccg gtccggcgat atttactttc
     6061 gcccggcttg ccggtgacat accaaactct gcaagcatcg cccggatccg cttccaggca
     6121 tctgccttca tcgccgcagc cggatgtgcc ttgatcatca catcgccatt ctgcgtttcc
     6181 gtgcggtagg tataaccctc aacatcgagt gtttcgcagt gatgccggta ttcggtgtag
     6241 gcttccacca gtaactcgag tgcacgcgca tcaagctgag aaatgatccc ttccgcattc
     6301 agctcttccg ccattcgcct gaaccagtac ttcccctgcg cccctaaatg ctgcggaatt
     6361 ttagggagac ctttttcgtc ctttttagcg gtttttttgg agtctttaac ggggcgcttt
     6421 gaggggttgc ctcgtatcaa atgcaggcgt ggcggggttt tcgggggtcc tgacataatc
     6481 ggttttacct atcaatcgtt tgatcgcatt cccaaaaaaa gttttcgaac ctgcggcgat
     6541 gcgaagaagg gttggcgggc ggtcccggac agccagggtt gcagggattt gacctgcccc
     6601 tccccacaag tgagaataat tatcacctga ttcgttcgcg cgctgttttc gctttgtggc
     6661 aaggccagca cagactctgc aggttgctgt ctgtgtctgt tccgccatgc gctttcggga
     6721 tgatgtggtc gacagttttc gcctcgctca ccacaccgac acgcagacac aactgacaca
     6781 gacctttatc gcgcttcaga atacgggcac gaatcaccgt ccattttaag ccatagccac
     6841 gctggtggcg gctcagtccg cgctggtgct gcacccagcc ttcactccga tgtttatcgc
     6901 agtagccaga gctgtctgtt gttgtgcctg cgcagcctcg cttacgacat gcgcggggga
     6961 tttgtgatgg catagaattt cgctcctcta aaaaatattc tgctctcacc gtcggtcagt
     7021 tctgcataca ctgccggaca ctatcaacaa tttcgcagac ctgagaagct gtatcgaaaa
     7081 gctgacgcgc cttatccagg ctgacgcatc ccgccaataa aaaaggcacc agtatcgcta
     7141 ccagtgccca tttcgccgtc gttcgcggca ttctgtgtgt ccagtgtttt ctgctcataa
     7201 cacaccaggt tatcagcgtt tcaactgaaa gtgaggtccg tctttcagtg ttttccagtc
     7261 cccgccccat tcgatggcag ttcccagctc tgcggcagcc tgcttaaatg cctgcgcgat
     7321 tttctcgtac agaggccagt cccatgacac ctggctgcca acccaggcaa caacatccac
     7381 cgcatcaccg gtcaggtggc ggctgttcat ggtctggctt tttccttccg caaccagttg
     7441 tttctgacgc tctttcgtgc gcagaccttc agtgatacca aaatcaacct ctgtcagttc
     7501 cagagcccgg cgaacgacag caaccagctg tggcttgacg cccaccagat tcttttcgct
     7561 gcgacgactg aatctgaatt tacccgacat attcacctca ataatggaaa gatttttgtg
     7621 acgttcccgc gagcgcgtac caccagcacg cagaacaaca gattaaaaaa cacctccagc
     7681 cagttcgccg ctaacgggcg accgcacaga tagctgagtg gtgcaaaggc atacagcagc
     7741 atcagcagcc aggccagcca tgacatcagc ggtttatgtc tggaatcacg gcgacgataa
     7801 aaaaagagcg tcagcacgat aaccgtgcat aacgccacat tcagtaatcc gggaaggtta
     7861 cttaacattg ccgcctcctc caccccgcag gcgggagaac aggccggaca ccagcgatgc
     7921 aatatcctgc tggtggatga acgagagaat cttcaccgac accaccgcca ccagcaccgc
     7981 acacagtgca tccgccgatg taccgtcata acctgttttt gatgcaatcc aggctgacag
     8041 cacatgcgca cccagcacac caacgataaa cgacaccaga aaatgcgccg ccacacgcca
     8101 ggctgaaagc gcctgcggca tcgttgccac aaataacgcc ccggcgaacg ccccaaacac
     8161 aatcccgaaa tccgttccgg taaacagccc gaataccgtc gccccgccga gcgccgcagc
     8221 cgtaccggaa ccggacaagg gttcagacat actttttctc ctgtaaataa aaaagggcca
     8281 ccagcggccc gtaaaaaaca cccgtcaaag gcacccacag ataccttttg tgtggtgtta
     8341 ttcagattta cgcagtaaag gccagagtac ggccagcgcc atcgccacca gcacaccatc
     8401 tgccagcacc gacatcagcc gtccggtgaa atccaccgcc actaccagaa acagcaggat
     8461 ggcagccagc acaaggcgcg cacttttcac aggtactgct ccagcggcaa ctgcagcgcc
     8521 tgagcaattt tcttgagctg cgcttcttca tccggaccaa tgccgtcctg gtcagcaata
     8581 tcgagacaca ggcacagcac atcaaccgct tcggcggttc ctgccacatc agccagttca
     8641 cgcaaagcct gagcattcgc actacgaggt gacgcttcat aacgggcgcg gatatttgca
     8701 ctcatttgtg caatctcacc ggagaacggc gcaaaggcag gaagtgctgc aatggttttt
     8761 tccagtacag cgatttcttt cgcatcacag gtgtcgtcgg cgaatgctat ggaatacgcg
     8821 ccccagacgg tcgcctccac tgcgtcgcgg ttctccatct tcttaacttc ggtaatggcc
     8881 ttgcgggttt tctttttgaa aataccaaac atcgtgactt ttccttttag tgggtgagcc
     8941 tcgccccggg atgggcagcc cacagagaaa gtcacactga ccatcccgta agctcacccc
     9001 tgaaaggctc tgtggttttt ttgatgtgcg ccgggcgtgg cgcggaaata aaaaaggccc
     9061 gccgtagcga gccgcataaa tactgaattc tgaataaaaa aaaaccgcct ggcgtggcgg
     9121 tctatggcat aaaatggcat gaagagaacg cgatgatttt attatgacca gagacgtcgg
     9181 gtgcctcccg aaacatccag tcctgcatgg atgctgtggt ttcccgcaaa accgactctt
     9241 tcaaccaccc tcgcactgag gaacgtctct ggggtgttat ttacaacacc ggaatgatac
     9301 attaccagcc ctgccaggaa atacaaatct ccaccagtaa tgcaccattc cactattgta
     9361 aaaaacagca ctggtaccgt aataaataat attcattaca gaacaggatg tggtaaggag
     9421 ttagggtaaa acatcccaca catataatgt gtcagtgcct gaaacagcct agcatcgtcc
     9481 tctgctggag cgggtagcgg gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat
     9541 agccattata cgatacccgc atatggtgcc gactaccgga atcgaactgg tgacctactg
     9601 attacaagtc agttgctcta cctactgagc t
//
LOCUS       DAZFRE010000151         9515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFRE010000151 DAZFRE010000000
VERSION     DAZFRE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.152"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(62..847)
                     /locus_tag="ACHKUI_004607"
     CDS             complement(62..847)
                     /locus_tag="ACHKUI_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600655.1"
                     /translation="MNLKKVCCICVLFSLLAGCASESPIDEKKKKTQTTQISINKNVP
                     QQLTDKDLYGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRKKTVTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
     gene            complement(1176..1252)
                     /locus_tag="ACHKUI_004608"
     tRNA            complement(1176..1252)
                     /locus_tag="ACHKUI_004608"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Asp,seq:gtc)
     gene            complement(1385..2116)
                     /gene="dnaQ"
                     /locus_tag="ACHKUI_004609"
     CDS             complement(1385..2116)
                     /gene="dnaQ"
                     /locus_tag="ACHKUI_004609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6600656.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2181..2648
                     /gene="rnhA"
                     /locus_tag="ACHKUI_004610"
     CDS             2181..2648
                     /gene="rnhA"
                     /locus_tag="ACHKUI_004610"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6600657.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2645..3367)
                     /gene="yafS"
                     /locus_tag="ACHKUI_004611"
     CDS             complement(2645..3367)
                     /gene="yafS"
                     /locus_tag="ACHKUI_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6600658.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3401..4156
                     /gene="gloB"
                     /locus_tag="ACHKUI_004612"
     CDS             3401..4156
                     /gene="gloB"
                     /locus_tag="ACHKUI_004612"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6600659.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4228..5586
                     /gene="mltD"
                     /locus_tag="ACHKUI_004613"
     CDS             4228..5586
                     /gene="mltD"
                     /locus_tag="ACHKUI_004613"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6600660.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5633..6403)
                     /locus_tag="ACHKUI_004614"
     CDS             complement(5633..6403)
                     /locus_tag="ACHKUI_004614"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6600661.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(6481..7281)
                     /gene="yafD"
                     /locus_tag="ACHKUI_004615"
     CDS             complement(6481..7281)
                     /gene="yafD"
                     /locus_tag="ACHKUI_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6600662.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7522..8436
                     /gene="yafC"
                     /locus_tag="ACHKUI_004616"
     CDS             7522..8436
                     /gene="yafC"
                     /locus_tag="ACHKUI_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6600663.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIQAFIDFLSGHVKTAPGGAVREA"
     gene            complement(8433..9236)
                     /gene="dkgB"
                     /locus_tag="ACHKUI_004617"
     CDS             complement(8433..9236)
                     /gene="dkgB"
                     /locus_tag="ACHKUI_004617"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6600664.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(9399..9475)
                     /locus_tag="ACHKUI_004618"
     tRNA            complement(9399..9475)
                     /locus_tag="ACHKUI_004618"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9439..9441),aa:Asp,seq:gtc)
BASE COUNT         2570 a         2251 c         2233 g         2461 t
ORIGIN      
        1 atagaaaaga gaaatgctct ttcttgctaa gtaccactaa aacgtctggt ataaaacctc
       61 actactgata tcgattcacc aggtctttaa ccactgctgc atacgttttc tgcttaagtt
      121 gtgaaatctg ctgttctgcc acatcaaggc tgtcagaatt tttattccct atctgcggaa
      181 aaatcacttt cgattcataa ttcacaggcg accaggtagc ccactcacct gtagccacat
      241 ccaccaacgt gaatcgaacc aaataacgca gtgacatcgt gtcagtcaat ttttgatttt
      301 tataatctgt ccaaactatt gatttcaatc cagagtcata ttttcctgtc tgcaacgtat
      361 cctggaaaac aataatcgct ttctgatggc cttttgctgc aacaaaacgc aatgcctgca
      421 tccagttcat attttcggca tctacaatat ccaggctttc atctttgttt ttgtctttat
      481 tacaggttac agtttttttc ctgtcaggaa tgccagaaaa tgtggcaact gtataatatt
      541 ttctcatctc ctcctgcata atggtttccg gtgcgcgact gccagactga acaagtatta
      601 caggagaatt aagaggcaca cgaaactcat ctccttcaag cgcagcttga atatcctcct
      661 cggatacagc cagtgtggtt tcattaccat ataaatcttt gtctgttagt tgctggggaa
      721 cgtttttatt aatagaaatt tgtgtggttt gagttttttt ctttttttca tcaataggag
      781 attcagaggc acatcctgca agcagcgaga ataatacaca tatacaacaa acctttttta
      841 aattcataat cggacactcc ctcgcctgtc ataatcatgg taatgtttag ctaacgattt
      901 ttaattatat actccacttg aatcagaaca aacaaaaaat aacagcaaat ataaattcat
      961 taaatcaata gaaaaatgaa aatcataacg tgcaaatata tttaaattga atgattcgat
     1021 agcatgatta aatattaatg acatggtcta tcatatgtga ttaacacatt tacagcataa
     1081 caaaatcata gaccgaagaa atagcgggat ttagatgaaa aaaccgcaaa attaaaaatc
     1141 ttgcggctct ctgaactcat tttcatgagt gaatttggcg gaacggacgg gactcgaacc
     1201 cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct ccgcgttgtg
     1261 ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac ggtttttctc
     1321 atcttttgaa tcgtttgctg caaaaatcgc ccaagtcgct atttttagcg ccttttacag
     1381 gtatttatgc ccgccagagg cagcttccgc ctttcttctg caccagatcg agacgggctt
     1441 catgtgctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt gcctgacgca
     1501 caatgcgctg aattgttgtt tcaccttgtt gctgttgtgt ctctccttcc atcgcaaaag
     1561 ccatcgacgt ttgcccacca gtcatcgcca gataaacttc cgcaaggatc tgggcatcga
     1621 gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc gtagcgggca cataacgcat
     1681 cgaggctgtt gcgcttaccg ggaaacattt tcctcgccac cgcaaggcta tcggtgacct
     1741 tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg tagtccataa
     1801 agccgatatc gaacgctgcg ttatggatca ccagctccgc gccgcggata tagtccatga
     1861 actcatcggc tacctcggca aacgtgggct tatcgagcaa aaattcatcg gcaataccat
     1921 gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca tggaagttat
     1981 tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc ttgtggcctt
     2041 catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg atctggcgtg
     2101 taattgcagt gctcatagcg gtcatttatg tcagacttat cgttttacag ttcgattcaa
     2161 ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc
     2221 tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga cgcgagaaaa
     2281 cctttagcgc tggctacacc cgcaccacca acaaccgtat ggagttgatg gccgctattg
     2341 tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac agccagtatg
     2401 tccgccaggg tatcacccag tggatccata actggaaaaa acgtggttgg aaaacagcag
     2461 acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgct ctggggcagc
     2521 atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac gaacgctgtg
     2581 atgaactggc tcgtgctgcg gcaatgaatc ccacactgga agatacaggc tatcaagtcg
     2641 aagtttaagc ctgtggttta cgacattgcc gggtggcacc aaccgcctgg cgaattcgcg
     2701 gtttgttctt gctctgtttc atcggattta gcgttaaagg aatagtccgt ttccgggcaa
     2761 caataagttg taagcaacca agcgcaggaa tatgcgcatt caatagtttt cctccgtgtt
     2821 tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac ttcaaaattc aacaaagaga
     2881 gccagtccag ctgccgcatc agagtgaaca tccggctgtt atagggcgat gttttgcgca
     2941 ataccggcac aagtttgcgt aatcccatta aactgatggg attgaagcca ctaatcacca
     3001 gccagccatc atcaatcagt acccgatcgg cttcacgcaa taaacgatgc ggatcagtgc
     3061 accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca aaaggaagat
     3121 gaagtgggtc cgcctgcacc tggacgggca ttccttgcgc agaaacattc acttgatgag
     3181 aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta agcagatgaa
     3241 aaccatacat tttagtgaac cacgggttga gctgacgctc cagcgccttg cgataaagct
     3301 ctccccaggg caaatcgccc cagcaatcag gagccacgac agtttgaggg actcttgccg
     3361 gtttcatcac aaccttccgt ttcacactga gaggtaatct atgaatctta acagtattcc
     3421 cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct gtctgattgt
     3481 cgatcccggg gacgcagagc cagtattaaa cgccatttcc gccaataact ggcaaccgga
     3541 ggccatattt ctcacccacc atcaccacga tcacgttgga ggcgtaaaag aactggtgga
     3601 aaagtttccg caaattgtgg tgtatggacc acaagagaca caagataagg gaacaacaca
     3661 ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg taattgccac
     3721 accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat tttgcggcga
     3781 cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg acaccatcac aaatgtatca
     3841 atcacttaaa aagttaagtg cgttacctga cgatacattg gtatgttgtg ctcatgaata
     3901 taccttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt ccataaatga
     3961 ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac ccgtaattct
     4021 gaaaaatgag cggcaaatta atgttttttt aagaacggaa gatattgatt taattaatgt
     4081 aattaatgaa gaaacattat tgcaacaacc tgaagaacgt tttgcatggt taaggtcaaa
     4141 gaaagatagg ttctgataaa actttcttgt catcggctcc gttcgccgtt atgatcggtc
     4201 gtcttttaag caactattga cacacacatg aaggcaaaag cgatattact cgcctctgtc
     4261 ctgctcgtgg gttgccagag caccggcaac gttcaacagc acgcacagag cctttctgca
     4321 gctggtcaag gggaagcagc aaagtttaca agtcaggcac gatggatgga cgatgggacg
     4381 tctatcgcgc cagatggtga cttgtgggct ttcattggcg acgagctaaa gatgggaatt
     4441 ccggaaaacg accggattcg cgaacagaaa cagaaatatt tacgcaataa gagctatctc
     4501 cacgatgtaa ctttacgggc agagccgtat atgtactgga tagccgggca agttaaaaaa
     4561 cgtaacatgc ctatggaact ggtactacta cccatagtgg agagcgcttt tgatcctcac
     4621 gcaacgtctg gcgccaatgc cgcgggcatc tggcagatca ttccgagcac ggggcgcaat
     4681 tatggtttga aacagacacg caattatgac gcgcgtcgcg atgttgttgc ttcaacaact
     4741 gccgcgctga acatgatgca gcgtctgaac aagatgtttg acggcgactg gcttctgacc
     4801 gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg caattaaaac gaacaaagcg
     4861 cgtgggaaat ccacggactt ctggtcgtta ccgttgccgc aggaaacgaa gcagtacgtg
     4921 cctaaaatgc tggcattgag tgatattctc aaaaacagca agcgttatgg cgtacgtctg
     4981 ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc tgagcagccc ggttgaaatg
     5041 gcgaaggttg cagatatggc ggggatttcc gtcagcaagc tgaagacatt caacgctggc
     5101 gtgaaaggct ccacgctggg cgcaagtggt ccgcagtacg tgatggtgcc aaagaagcat
     5161 gcagatcaac tgcgtgaatc tctggcttcg ggcgaaattg ctgctgtaca gtcgacgctg
     5221 gttgccgaca atacgccgct taacagccgt gtttacaccg tacgctctgg cgacacgctt
     5281 tcaagtatcg cttcacgtct cggcgttagc accaaagatt tgcagcagtg gaacaaactg
     5341 cgcggatcta agctgaagcc aggccaaagc ttgacgattg gcgcaggtag tagcgcacag
     5401 cggttggcaa acaacagcga tagcattacg tatcgtgtgc gcaaaggcga ttcgctttca
     5461 agcattgcta aacgccacgg cgtgaacatc aaagatgtga tgcgctggaa cagcgatact
     5521 gcgaatctgc aaccaggcga taagctgacg ttgtttgtga aaaacaacaa catgccagat
     5581 tcctgacaaa ccagataatg aaaggcaccg attcccccgg tgcctttttt atttatgctg
     5641 ctttatgtgc ttctaccatg atgatatcac tggtgaaaga gccatcattc tgcaaggcaa
     5701 aatacgtttt cacctctgtc gatgcgctct gttggtaaat gcgaatagcg tctactaacg
     5761 cttctggcgt acgcattctc gcgacccatg aagaaaattc cagcggtaac ttatctgtaa
     5821 ttaaattatc aactatcaga ttggcttcat tgattaacct caaccactca ccgctggcgt
     5881 agtttcgtac gtgagaggta tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca
     5941 ctgggtggcc cggagacatt acgtccatca caatcagcct accgccaggt ttcaatatcc
     6001 ggttcacttc acgcagtgct gcaccaacat catgccagtg atgggcagaa taacggctga
     6061 taacaatatc aaatgcgtta tcggcaaatg gcagactttc ggcataaccc tggcgggtgg
     6121 tgatattttt cagttgccgg gcttcggcag cttgtgccac gacatccagc atttgggcag
     6181 ataagtcata cgctaccacc gcgctcacgt tttgcgcagc aacaaagctg gcatgccctg
     6241 ctccgcagcc catatcaagt acacttgcat caggataatc agccagacgc accgccaggc
     6301 gctgtaaatc tcgtccggat gcatgtacgg tactggtcag atattcgctg gcttgagagc
     6361 taaactgctt ttctacgtgg tcatggtggg attgtgttgt cattgcgctg tccttttgtt
     6421 gtttgagtat aaaagggcag cgccaaacgt tggtctgccc tgtggcagac ctgacatacc
     6481 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     6541 cgtaaccagt acagaagctt cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     6601 gcgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgta gcgacatttc
     6661 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     6721 cataatgacc gggccactgt ggtgagctat ctgatcgcca ataggaagta actgcttact
     6781 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     6841 ggtgtcaggt aagggataga ccgtcaccag tgccgatttc gccagacgca aaatgggttc
     6901 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     6961 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcagtcgc
     7021 aaactgtact aactctggcg ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt
     7081 gccgtagttc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     7141 caggatccga atacgctctt cggtacttaa cggttctcca ggtggtaatg cctggccgat
     7201 gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt tgtccggcaa catagcgcat
     7261 ggcataggtg ttttttcgca cttttgactt tgaaacctct atcacgtcaa cccttccgca
     7321 ctcgggaggg ttctactgtt atagggattt tagctcactc tttcaacgag caattgtata
     7381 ttgttatgta agcaagtgct tacaccggtc aggattttgc tcattattga gaacattaaa
     7441 tcggatggca tatctcgcat cctgatggtt taatgacaaa taaattcaaa atatctcttc
     7501 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttca
     7561 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     7621 gtaagccggg cggtgaaaaa gctggagatg aaacttggcg ttagcctgct taatcggacc
     7681 acgcggcaac ttagcctgac ggaagaaggc gagcgttatt ttcatcgcgt acagtcaatt
     7741 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     7801 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttgattaag
     7861 ccttttcgtg aacgctatcc ggaagtcact ttatcgctag tctcctccga aacgattatt
     7921 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgccg gtacgttaac ggattccagc
     7981 ttacgtgcca ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     8041 tcctgctacg ggaagccaga aacgatcgac gatttaaagc aacatatttg cctgagattc
     8101 actgaacccg cttccctcaa tacctggccg atagcctgta gcgatggaca attacatgag
     8161 gtgaagtacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     8221 aacgggattg cgtgtttgtc cgactacatg atcgacaaag aaatcgctcg cggagaattg
     8281 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc agtctattac
     8341 agcgaccgtg cggtaagtac gcgcatccag gcttttatcg atttccttag cggacatgta
     8401 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc
     8461 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     8521 agctgtaaat tttgtgcctt aagattactc tccaggtttt cacgtttagt agaagaagga
     8581 attactgagt aaccttcccc catagcccac gccagaatca cttgagccgg agtcgcattg
     8641 tgtttagctg caatacgagc aataacctta tctttcagtg cattgccata cgccagagtc
     8701 atataggaag taatatggat accgtgctgt ttagcccaag cgaccacttt acggttttgc
     8761 agataaggag agagttcaat ctggttagta gcgatgtttt cagcgccaac agcagcaatc
     8821 gctttttcca tcaatgggat cgtgaagttg gaaataccga tctcacgcgt cagcccttgt
     8881 tttttggctt ccagcagcgc ctgcataaac tcttcagcag agacttcatc gcttggtgac
     8941 ggccagtgga ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttc
     9001 agactcggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     9061 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     9121 tagatttgtg cggtatcaat tgcgcgataa tcaagttcaa gcgccgtttt cacagatgaa
     9181 ataacaacgt cgtctttcag acggaaagtg cctaaaccaa atgcagggat agccataata
     9241 tgcctctttt aatgctattt tcgttaactg aggtgattat gggcttcaga cagttgtgga
     9301 aaaatggcgt aaaacgcaga ggatttttgc gattttggca ataatagaaa tacaaacgaa
     9361 aaaaaacctc gagcgttaac tcgaggcttc ttaataagtg gcggaacgga cgggactcga
     9421 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga
     9481 attcttttac aaccaccggt ttataaccgg cttac
//
LOCUS       DAZFRE010000152         9343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFRE010000152 DAZFRE010000000
VERSION     DAZFRE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.153"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(37..891)
                     /locus_tag="ACHKUI_004619"
     CDS             complement(37..891)
                     /locus_tag="ACHKUI_004619"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO6600665.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1233..2063
                     /locus_tag="ACHKUI_004620"
     CDS             1233..2063
                     /locus_tag="ACHKUI_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6600666.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2095..3030
                     /locus_tag="ACHKUI_004621"
     CDS             2095..3030
                     /locus_tag="ACHKUI_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6600667.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3123..3365
                     /gene="yhdT"
                     /locus_tag="ACHKUI_004622"
     CDS             3123..3365
                     /gene="yhdT"
                     /locus_tag="ACHKUI_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6600668.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVSAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3355..4806
                     /gene="panF"
                     /locus_tag="ACHKUI_004623"
     CDS             3355..4806
                     /gene="panF"
                     /locus_tag="ACHKUI_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6600669.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            4818..5699
                     /gene="prmA"
                     /locus_tag="ACHKUI_004624"
     CDS             4818..5699
                     /gene="prmA"
                     /locus_tag="ACHKUI_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6600670.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6028..6993
                     /gene="dusB"
                     /locus_tag="ACHKUI_004625"
     CDS             6028..6993
                     /gene="dusB"
                     /locus_tag="ACHKUI_004625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6600671.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7019..7315
                     /gene="fis"
                     /locus_tag="ACHKUI_004626"
     CDS             7019..7315
                     /gene="fis"
                     /locus_tag="ACHKUI_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6600672.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7401..8285
                     /gene="yhdJ"
                     /locus_tag="ACHKUI_004627"
     CDS             7401..8285
                     /gene="yhdJ"
                     /locus_tag="ACHKUI_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6600673.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVAPDLIAK"
     gene            8369..8548
                     /gene="yhdU"
                     /locus_tag="ACHKUI_004628"
     CDS             8369..8548
                     /gene="yhdU"
                     /locus_tag="ACHKUI_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6600674.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8551..9213)
                     /gene="acrS"
                     /locus_tag="ACHKUI_004629"
     CDS             complement(8551..9213)
                     /gene="acrS"
                     /locus_tag="ACHKUI_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6600675.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         2358 a         2246 c         2289 g         2450 t
ORIGIN      
        1 taaacaatcc tcattatttt tgtagggtac aacgtcttaa atttttcctt cactaccaca
       61 gacgccaata atacgagtta cgacttcttt catggcggcc tttgcgggct gcatatagtg
      121 acgtggatca tttgccgcag gattgttgta gaaatatcct tttaacgcat cggcaaacgc
      181 aattttcaat tctgtcgcga cattcacttt acaaataccg cgacgaatac agtgctgaac
      241 gtcctgttcc gataagcccg aagcaccatg caacaccagc ggaatctcca ctcgctgacg
      301 aatctgttcc agccgcacaa aatccagtcg tggttcatgc gcatataaac catgtgctgt
      361 gccgattgct accgccaggg agtcaatttg ggtacgttga acaaactctg cagctgcggc
      421 tggtgaggta taagcggaat ctttttcatc cacccgcaga tcgtcctcct ggccgccaag
      481 acgccctagc tcggcctcaa cgctaacatc aaagcgatgg caaaaggtgg taacttcttg
      541 cactcgcgca atgttgtctt caaatggcaa atgggagcca tcaatcatga cagaacggat
      601 ccctgcgcga accttgttaa aaatgtcatc actctcttca tgatggtcga gatgcaggac
      661 taacggcagg ttgtactcgc gagccagcga ttgtactaac gaaattaaac ttcccacgcc
      721 accataacga tatgtaccgg gtgttcccgc cagcataacg ggagagcgaa gttgtgcagc
      781 gctctcaaca actacctgta aagtttcaag gttatgaatg ttgaaagcag ggacggcata
      841 tccctctgac tgggcttttt tcagcatgtt tttgcttgaa ataatataca tcgcagctcc
      901 tgttatttgt cgttcgcctt acaaaatgaa gaaagagaat cttcactttc attttgaaag
      961 caaactagca cagcaaaagc agcaaaaagc gtgatgcatg tcgaacatca tcaaaataac
     1021 ttacaaaaaa taacttaatt tatttttcac tttgcaagtg aaaccaatga aaaccctgca
     1081 taaatatgaa tatattcatt tatttttcaa tgtattaagc tattttcgtt gcaatttttg
     1141 acccatgatt ttcttttcga attgtcgata tgattgaagg ataactttca aagaaaaata
     1201 caaggggcta tagtgttacg cattaaaggc caatgaagag tgttaacgtg ccgagaaaat
     1261 cgtctaacct actgaaacgc agattaaaaa ttgcagaaat tgttgccgcc cagggtgaaa
     1321 tcaaagttga tgacttatct gcgcaacttg gtgtctctgg cgtgacgatc cgcggcgatc
     1381 tcagttatct ggagcaacag ggatatttaa agcgatcatt tggcggtgct attgcgacac
     1441 cactctcctc tgaaccagaa gcgatacaac cgatcgcgcc attgtctctg gcacaacaaa
     1501 tggaaatagc acgacattgc gcacgattaa ttcgcgatcg tgacacgcta tttcttggac
     1561 atgggacaat ctgccgtaag atcattccat tgctcagcga agtgaaaaaa ctgcgtctga
     1621 tcaccaacga tcccgaacat gcgctgctgg caaatcagtt tattgatggc gaaataatac
     1681 tggctggcag tgaaatgttg cgccccgaca ccgcactggc cggaaaacag cttgaacagg
     1741 cacttcagca tttcaccatt acacatagca ttctggaaat cagccatctg gatgctgatg
     1801 gtactttaaa tatcaacgtt cccagactcg ccgctgcctg gcagctctgt ttcgatcatg
     1861 cccagaataa gacggttatc gttgccgccg atcctgcgcc atcaacggct gctatcgggc
     1921 atttgcatca ggcagaaaat gtgatcgtca gccgcagcat taacgaacaa tatcagcaac
     1981 aattgcagtc ggctgatttc gttatccttt ataccagtaa tgagtgcttt acctggtcca
     2041 atcgcgaaag gcttcaggaa tgagaggggt tttgtgacaa caactatctg cgctatgggc
     2101 gaattgctgg ccgagttttt gtcccgcaac ccacatcaaa aattcactca gcctggggag
     2161 tttatcgggc catttcccag cggtgcgcca gcaatttttg ctgctcaggt ggcaaaactg
     2221 tcccatcggg ccatcttctt tggatgtgtt ggtaatgatg attttgcccg actcattata
     2281 gagcgtctcc gtcatgaagg tgtcattacc gatgggatcc atgttatgaa caatgccgtc
     2341 acaggtacgg cgttcgtgag ttatcaaaat ccccagcagc gggatttcgt ctttaatatc
     2401 cctaacagcg cctgcggttt gtttactgcc gagcacattg ataaggatct gcttaaacag
     2461 tgtaaccatc tgcatattgt gggctcatcg ttgttctcat ttcgcatgat cgatgtcatg
     2521 cgtaaagcaa taacgacgat caaatcggct ggcggcaccg tttctttcga tcccaatatt
     2581 cgcaaagaga tgctgagcat tcctgaaatg gcgcaggctc tcgattattt gattgaatat
     2641 acggatattt ttatccccag cgaaagcgaa ctccctttct tcgcgcgtca caaaaatctg
     2701 tcagaggaac agattgttag cgatcttctc cacggcggcg taaaacatgt ggcgataaaa
     2761 cgcgcccagc gtggggccag ctattacaag cttaaaaacg gtacattaca cgcccagcat
     2821 gttgcaggtc acgatatcga aattatcgat ccaacgggtg caggcgactg ctttggcgca
     2881 acgtttatca ctcttttctt atccggtttc ccggcacaca aggcgctgca atatgcaaat
     2941 gccagcggcg cgctcgccgt aatgcggcaa ggtccgatgg aagggatatc ctcactggca
     3001 gacattgaag actttttgca gcagcactga acggcaattt tctgtcagat cattttccct
     3061 gcctgaagac ttgccgtaga attgcccgtt tctcgtatcc ccccatgagg aggacccctt
     3121 ccgtggacac tcgttttgtt caggcccata aagaggcgcg ctgggcgctg gggctgaccc
     3181 ttttgtatct ggcggtatgg ttagtatccg cttacttacc tggcgttgcc cccggtttta
     3241 ccggctttcc gcgctggttt gagatggcct gcatcctgac gccgctgctg tttattggac
     3301 tgtgctgggc gatggtgaaa tttatctatc gcgatatccc actggaggat gacgatgcag
     3361 cttgaagtaa ttctaccgct ggtcgcctat ctggtggtgg tgttcggtat ctctgtttat
     3421 gcgatgcgta aacggagcac tggcaccttc cttaatgagt atttcctcgg cagccgctct
     3481 atgggcggta ttgtgctggc gatgacgctc accgcgacct atatcagtgc cagttcgttt
     3541 atcggcgggc ctggagctgc ttataaatac ggcctgggct gggtattgct agcgatgatt
     3601 cagcttcctg cagtctggct ttcgctcggt attctcggca agaagttcgc aattcttgcg
     3661 cgccgctata atgcggtgac gctgaacgat atgctgtttg cccgctacca gagtcgtctt
     3721 ctggtatggc tggcgagttt gagtttgctg gttgcgttcg ttggtgcgat gaccgtgcag
     3781 tttatcggcg gtgcgcgcct gctggaaacc gcggcgggta ttccttatga aaccgggctg
     3841 ctgattttcg gtatcagcat tgcgttgtat accgcctttg gcggctttcg cgccagcgtg
     3901 ctgaacgaca ccatgcaagg gctggtgatg ctgattggca ccgttgtgct gcttattggc
     3961 gtggtacatg ccgcaggtgg tttaagtaat gcggtagaca cgctgcaaac catcgatccg
     4021 caactggtta cgccacaagg cgctgacgat attctgtcgc ctgcctttat gacgtcgttc
     4081 tgggtactgg tgtgctttgg cgttataggc ctgccgcata ctgcggtgcg ctgtatctct
     4141 tataaagaca gcaaagccgt acatcggggg atcattatcg gtacgattgt agtcgcaatc
     4201 ctgatgttcg gtatgcatct ggccggagcg ttaggtcggg cggtgatccc cgatctcacc
     4261 gtaccggatc tggtgatccc aacgttaatg gtaaaagtgc tgccaccgtt tgctgccggg
     4321 atcttcctgg ctgcgcctat ggctgcgatc atgtcgacaa ttaacgccca actgctgcaa
     4381 agttccgcta cgatcattaa agatctctat ctgaatatcc gtccggatca aatgcaaaac
     4441 gagacgcgtc tgaagcggat gtcggcggtg atcacgttgg ttctcggtgc gttgctgctg
     4501 cttgccgcct ggaagccgcc agaaatgatc atctggctga atttgctggc cttcggtggg
     4561 ctggaagccg ttttcctgtg gccgctggtg ctgggtcttt actgggaacg cgccaacgcc
     4621 aaaggcgcgc taagtgcgat gatcgttggc ggcgtgctgt atgccgtact cgcgacgctg
     4681 aatattcagt acctgggctt ccaccctatc gtgccctcgt tactactaag tttgctggct
     4741 ttcctggtcg gaaaccgttt cggtacatcc gtcccgcaag ctaccgtttt gactactgat
     4801 aaataaagag ttttgccatg ccttggatcc aactgaaact gaacactacc ggcgcgaacg
     4861 cggaagatct tagcgatgcg ctgatggaag cgggtgccgt ttctatcact tttcaggata
     4921 cccacgatac gccagtattt gagccgctgc cgggcgaaac gcgcctgtgg ggcgacaccg
     4981 atgtgattgg tctgttcgac gctgaaaccg atatgaacga cgtggtggcg attctggaaa
     5041 accatccgct gctcggcgcg ggcttcgcgc ataaaatcga acaactggaa gataaagact
     5101 gggagcgcga atggatggat aatttccacc cgatgcgctt tggtgaacga ctgtggatct
     5161 gcccaagctg gcgtgatgtg ccggacgaaa acgccgtcaa cgtgatgtta gacccagggc
     5221 tggcgtttgg tacgggtacc catccaacta cctctctgtg cctgcaatgg ctcgacagcc
     5281 tcgatttaac cggtaaaaca gtcatcgact ttggctgcgg ttccggcatt ctggctattg
     5341 cggcgctgaa actgggtgca gcaaaagcca ttggtattga tatcgatccg caggcgatcc
     5401 aggccagccg cgataacgcc gaacgtaatg gcgtttctga tcgtctggaa ctctacttac
     5461 cgaaagatca gccagaagaa atgaaagccg acgtggtggt cgctaacatc cttgcaggcc
     5521 cattacgtga actggcaccg ttaatcagcg tcctgccagt ttcaggcggt ttgctgggcc
     5581 tttccggtat tctggcaagc caggcagaga gcgtttgcga agcttatgcc gacagcttcg
     5641 ctctggaccc ggtcgtggaa aaagaagagt ggtgccgcat taccggtcgt aagaattaac
     5701 cttcgcatcg ccgtatggcg acgcagagcg aagtgcgagc gatgtcacaa aaggcacgtt
     5761 aatttgccga ttatttacgc aaatttgcgt gccaaaattt tcattcataa agaaaaattg
     5821 agaacttact caaatttctt tgagtgtaaa ttttagtcac tattttctaa tatgatgatt
     5881 tttatgagta attatcgcgc tacgctcatt ttaaatgcaa ttctttgatc catctcagag
     5941 gattggtcaa agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc
     6001 agtcacagta tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc
     6061 ctgatcgcag cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag
     6121 atgggagccg gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc
     6181 gacaaatctc gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt
     6241 gctggtagcg atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc
     6301 cagattattg atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt
     6361 tcagccctct tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca
     6421 gtggacgttc ctgttaccct gaaaattcgc actggctggg caccggaaca ccgtaactgc
     6481 gaagagattg cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt
     6541 acacgcgcct gtttgttcaa tggagaagct gagtacgaca gtattcgtgc agttaagcag
     6601 aaagtttcca ttccggttat cgcgaatggt gacattactg acccgcttaa agccagagct
     6661 gtgctcgact atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc
     6721 tggatctttc gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct
     6781 ttagcagagg ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt
     6841 ccggcaaaag ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct
     6901 ccaaatgacc agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag
     6961 gcgttggagg catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat
     7021 gttcgaacaa cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca
     7081 ggtaacccaa aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca
     7141 actgaatggt caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc
     7201 cctgttggac atggtgatgc aatacacccg tggtaaccag acccgtgctg ctctgatgat
     7261 gggcatcaac cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag
     7321 gttagctaaa tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag
     7381 gtgtcacaaa gggagtgacc atgagaacag gatgtgaacc gacccgattt ggtaatgaag
     7441 caaagaccat catccagggt gatgcattaa ccgaacttaa aaagctcccc gccgaaagtg
     7501 tcgatctgat ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg
     7561 aagcctggaa agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccatc
     7621 gcgttctgaa aaagcagggc agcatgtaca tcatgaatag cacggaaaac atgcccttta
     7681 tcgatctcca gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca
     7741 gttctggagt gcaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg
     7801 tgaaagacgc aaagaactac acgttcaacg gtgatgctat tctggtagaa gccaaaaccg
     7861 gatcgcagcg cgcgttgatc aattatcgca aaaatcctcc acagccttac aatcatcaaa
     7921 aagtaccggg taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaaa
     7981 accacccgac gcaaaaaccg gaagccttgc tgaaacgcat tattctcgcc tcttccaacc
     8041 caggcgatat cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccgtcg
     8101 ccagcgggcg aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc
     8161 gacggctgga tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa
     8221 agaccggcaa cctgtcaaaa cgaagccggt taagcgaagt tgcccccgat ctcattgcaa
     8281 agtaaaggaa gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga
     8341 cttggttaaa catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt
     8401 cgctgtcttc gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac
     8461 agaaacagac aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga
     8521 cgaactgaat tttcaggaca gaatgtgaat ttatatgaca cttaattcat tcgttttatg
     8581 aattaatttc gttatgtttt catctggcat gaacattctt aatacgttat cgaccagagc
     8641 gggggcttgt ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc
     8701 gctgaaggca ccatcaataa taatcatcac aacatctaaa tcgagattat ttgctacaca
     8761 accttgttgc tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt
     8821 ttcgcgtatc actccctcgg ccagcatctc atcattaaat tcacatttgt gatataagat
     8881 tttcagcaac gcctgctggc ggggaatttt ggcaatatat tgcaaaccga caatcaattt
     8941 ttcacgcaat tgctgaaacg ggtcatgctc taacccagcg gtcaagtgat cctggattaa
     9001 ctcccgcaat gaaggctgtt gcaaccacat ctcattaaac agttgagtct tgttttcgaa
     9061 gtgccagtag atagcgccac gcgtaacgtt agcagcgtcg gcaatgtcgt tgagcgtcgt
     9121 cttgcttacg ccatgctgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt
     9181 cttcagggct tcagctttgg ttctttttgc catgattaat tattcaggaa ataaatatat
     9241 tcgacacagg atgagaaaat agcgaaggtt aatctatcac ctaatgtgta tttatacgag
     9301 aggctaatat tgcgttgcta taaatcgtca aataaatgat atg
//
LOCUS       DAZFRE010000153         9331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFRE010000153 DAZFRE010000000
VERSION     DAZFRE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.154"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            618..1589
                     /locus_tag="ACHKUI_004630"
     CDS             618..1589
                     /locus_tag="ACHKUI_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO6600676.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFGTLSPEQLEMLHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            1653..2126
                     /locus_tag="ACHKUI_004631"
     CDS             1653..2126
                     /locus_tag="ACHKUI_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6600677.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            2123..3394
                     /locus_tag="ACHKUI_004632"
     CDS             2123..3394
                     /locus_tag="ACHKUI_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6600678.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3437..5101)
                     /gene="tsr"
                     /locus_tag="ACHKUI_004633"
     CDS             complement(3437..5101)
                     /gene="tsr"
                     /locus_tag="ACHKUI_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6600679.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            5478..7628
                     /gene="btsT"
                     /locus_tag="ACHKUI_004634"
     CDS             5478..7628
                     /gene="btsT"
                     /locus_tag="ACHKUI_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6600680.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            7746..7949
                     /gene="yjiX"
                     /locus_tag="ACHKUI_004635"
     CDS             7746..7949
                     /gene="yjiX"
                     /locus_tag="ACHKUI_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6600681.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            7960..8916
                     /gene="yjiA"
                     /locus_tag="ACHKUI_004636"
     CDS             7960..8916
                     /gene="yjiA"
                     /locus_tag="ACHKUI_004636"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6600682.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(9002..9193)
                     /locus_tag="ACHKUI_004637"
     CDS             complement(9002..9193)
                     /locus_tag="ACHKUI_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO6600683.1"
                     /translation="MSMVKHKRGTLYTLDVHQEAELKALVNKSDEHIDYSDIPASGSE
                     AWADATRGNFLPDPGIHAQ"
     gene            complement(9234..>9331)
                     /locus_tag="ACHKUI_004638"
     CDS             complement(9234..>9331)
                     /locus_tag="ACHKUI_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HFO6600684.1"
                     /translation="FDELKCKKGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         2224 a         2427 c         2274 g         2406 t
ORIGIN      
        1 cacttaacgt taatcattaa tcactgcgtt cactcacatg atcagaagga acccagtaaa
       61 ataagttttc aagttgataa caaattcacc atgtgaatat ttactacctt tccccgttgt
      121 aacgaatcat ccgttactct ctgatttcct tgccgaacaa gttatttagt gaatgacggc
      181 tttcttttaa catcagcatg aattatacgt ttagcaaatg tattaaatca gcactaaata
      241 aatttaatgg ataccaccat tctgcgtcaa cacttagttt ttatctgtat tgacaattaa
      301 aaataaccgc agccaaatta atgattttat ttttaataaa tcatcaaccg aataagagat
      361 cttatcagcg ataactaaac tcatcagagg gtgttccttg taacgtcatg cattacataa
      421 ctttaagata tacaatcaaa aaatgcatta aaaaccgatt aaaagcagca aagtattacc
      481 cggtgcacga atttatgata catctgcagc cattctgcta ttcgctgact acttttcagt
      541 aatgactgct ttttcataag gtttcactcc cgacaacaat gttggagttt tatttcctta
      601 gcatggagta agtaaagatg agcaaaataa tagcgatgct aattactgtc agtacccttt
      661 ttttaccgtc aataatccag gcaaaaccta tttcaataaa agttgcctat gaaaataacc
      721 ctggagaacc cctggatgtg gtcatgcgtt actgggcaga cctattaaat aaaaaaagta
      781 atggcgaaat aacacttgcg ctctatccca gctcccaatt gggatcaaaa caggatgtca
      841 ccgagcaagc catgatgggc atgaatgtca taaccttgag tgatgtggct tttcttgccg
      901 attatgaacc cgatttgggg attttgtttg gaccgtatct gactgacgat ccgcaaaaac
      961 tgttcaaaat atatgaaagc gactggttta aacaaaaaaa tgaaagtctg aagaaaaagg
     1021 gaattcatgt ggtaatgaat aattatctct atggcactcg acagattatt tcgaaaaaac
     1081 ccattcgcac cgttgacgat ctcgcaggtt taaaaattcg cgttcctaat aacgtcatgc
     1141 aaataaaagc cattcaggcg atgggggcaa caccaactcc catgccatta ggcgaagttt
     1201 atcccgcatt aacccagggc gttattgacg gcgtagaaaa cccaatttca gtgctgcaag
     1261 ggcaaaaact gtatgagcag gcaagatatc tgacaatggt taactacctg acaaatacct
     1321 cagtgtggat aggtggtgaa gccttctttg gcaccctttc accagaacaa ctggagatgc
     1381 tccaccagac cggctatgaa gcgggtgtct atagccagaa actcactctt gagcgtgatg
     1441 ccgaaatgtt gaaaaccatg caggcagccg gcgtcgaagt gatctatccc gatactggac
     1501 cattccagaa gaaagcacgc gaagtttact ctcaattccc ggaatggaca ccaggtctgt
     1561 acgaaaccat tcaacagcaa ctgcaataag cattaatgct cagcgtcctg cctcatctct
     1621 cttaggcagg acacgcacta acaggaggtt tcatgcgtct ttttggcaaa gtggcagaat
     1681 ttagtgctgc ctttgcgcta ttcgttctgg ttgtggtgac aattggagcg gtatttatgc
     1741 gttatttcat cggccaaccg cttcaatgga cagaagaaat gtcgggaatg ctgatgattt
     1801 gggtggtgat gttaggtgga gtggtggcgg aacgtgacag ggcgcacctg accattccct
     1861 tcctgatgga gatgcttcca ggcaaactca ggcgtgttat tgcagtactg gtcgcccttc
     1921 tctccatcgc actgctgctt tatatggcct ggcttggcta ccgactagcc gaaatggcgc
     1981 agtttaaagt gacacaaatt ctgaaggttt cctggttctg gatcgatctg gcggtccctg
     2041 tcggcgcact ggcaacggct atttataccc tctactggtt aataaacgat acaaaacaat
     2101 ccgatagcgg agacaaaatg caatgagttg gttattgatt attccgctaa tgatcgtctg
     2161 tttgtttctc aacattcggg tctatctggc gatgtttatt ggcatcatcg cttacttttt
     2221 attcttctcc agcgtaccga ttgaaattgc cgtgcaaaga ctaatcgcgc caacgcaaag
     2281 cccatcattg ctggcaattc cgttttttat cttgctcggc acactaatga gctacaccgg
     2341 cattgccgaa cgcatcctgc acattgccaa cgttctggtg gggaaaatgc gcggcggact
     2401 gggcgtggca aacattctcg tcagcaccat gatgggcggc gtcagcgcct ctaaccttgc
     2461 tgacgcggcg atgctgtcgc gcatgatggt gccggagatg gagcgtaaag gctacaatcg
     2521 cgcgtttgcc gccgccatta ccgctggtgg ctcgttagtc accccgatca taccgcctgg
     2581 aatagccctg attatttacg gtctggttgc cgacgtctct atcggaaaaa tgtttatcgc
     2641 cggactggta cctggtttgc tatgcgcagt cgtactgatg tttaccgtct accttgtcgc
     2701 ccgcaaaact aacgctaaac cctcgcgtga aagctggcct acgggcaaag aggtggtgtt
     2761 ctcgctggga caggcctggc ctgcgctgtt cctcattttt gctgtggtgg gtggtattcg
     2821 cgctaacatt ttcaccccaa cagaagcagg tgcagcagcg gtgctaatcg tgctggcgat
     2881 tggctttttt atctatcgtg aaatgcggat ttctcatgtc gtcaaagcat tgggcgaaac
     2941 ggcacgcgca accgcatcgg tgatgctggt aatcatggcc agcgccgcat tgggatggat
     3001 tttctcaatg gaacatgctg gtgtcgcagt ggcgaatttt gtgacttcgc tgaccgaaaa
     3061 caaatacatg ttcctgttga ttatcaacat attgctactg acgctgggga tgtttctgga
     3121 aggaaacgcc attttgctga tcctggtacc actattaaaa ccggtggtcg aacactttgg
     3181 catcgatccg gtgcattttg gcatcatcat gatcttcaac ctgtcgattg gcgcattcac
     3241 gccaccggta ggtaccgtga tgctgctggt atgtaatatc acgcaagttt cggtgggaaa
     3301 tttctttaaa caatccctgc ccctactggc ggctctactg ttaatgctaa tgctggtgac
     3361 atatatcccg gcgatttcac tgttcctggt ttaataataa aaacgccgga tgaaatactc
     3421 atccggcatc attacgttaa aacgtttccc agttctcctc gctatctgcc acggccattt
     3481 tgcgcggcgt agctggcgtc acggttttta ccacagccga tgcttcacgc tgctgttgct
     3541 gctgttgctg aatccggaac actgccactg cttcggtcag gcgactggcc tgctcttcca
     3601 gcgcggcggc ggcggcggca gattcttcca ccagcgcggc gttctgttga gttacccggt
     3661 ccatctcagc aaccgctaag ccaacctgat cgataccacg gctctgctca tcagaagcag
     3721 aagcaatttc gcccataatg tccgtcacgc gggtcacggc actgacaatc tccgccattg
     3781 tttccccggc gctttcgacc agcgtagagc caacatccac tttccccacc gagtcttcaa
     3841 tcaggctttt aatttcacgc gcagcctgag cgctacgctg ggccagatta cgcacttctc
     3901 ccgcgaccac cgcaaaacca cgtccttgct cacccgcacg cgcagcctca accgccgcgt
     3961 tcaaagccag aatattggtc tggaaggcaa taccgtcaat tacgctgata atatcggcga
     4021 ttttctgcga actggtggag atatcgcgca tggtctgcac cacgttatcc accactttgc
     4081 caccgcgttg cgccgtttcg gaagcactta acgccagatg gctggcctgg cgcgcatttt
     4141 cggcattctg tttcaccgtt gcggtcagtt gctccatgct ggctgccgtc tcttccagcg
     4201 aagcggcctg ttgctcggtg cgcgaagaga gatcgttatt gccggtggcg atttcgctgg
     4261 caccgctata gatggcattg gccccgttgc gcacatcacc gacggtacgc atcagctctc
     4321 cctgcatatg gcgcaaactc tctgccagtt gccccatctc attagagcca tccacctcaa
     4381 tcggtttcac cagatcgccg cctgcaatat gacgaatgct gtcaatcagg cgattcattg
     4441 gcgcgaccag cgaggcttta ataccgaacc agacggcgag gatgaccgcc agtacgacga
     4501 tcatcacgcc taccagaatc cacatcgcct ggctgtagga ggcattgtta tcgctgacgg
     4561 cgatatcata gagccgatcg ttttgctcca tgtaagccac atactgcttc tcgaaaccgt
     4621 cctgatatcc ctgggtcggc tgatcaaaga actcgttgat tttgcctgca cctaacagtt
     4681 gtatcagctc cgccagcgca ttgtgataaa tatcgtaatt acgtttgatc tctgccgctg
     4741 cggcggtgct ctgacgcggg tcacgcggca acgcttcgta atccgcccag tttttttccg
     4801 cctgtttcag cgaaatactg gcactctgca tcaactcagc aacggttgaa ccgctaccaa
     4861 tattgttctg atccatcatg tagcggatac ccgcgcggtt gagggtgtta cgcgtctgca
     4921 acaacgcgac ccagctgcca ttcagcgtgg attgctgctg gcgaatggtt tgtaaaacag
     4981 tgaaattttc tttgtcgttc tttaaggcat taaagaacag accgcctgaa gtcagttgta
     5041 aaaggccaaa aacggccaaa accagcagta agctggtcac aattttgata cgttttaaca
     5101 tggtttctct ttcctgtgga ccgatgcaag attttcggcc tggaaaggaa aaactttatg
     5161 aaaatcccgc ggagatgaca tcgagtaatt ttattcaccc cctgaattta aaaagccatt
     5221 atttacataa attattcgca atataaatat gtgatataaa tcacatattt atggttactt
     5281 aaacgacgcc tttgccgctc accgccattc ctgaccgctt acgtccctaa aataaccact
     5341 cagttattta ccttacttta cgcgcgcgta actctggcaa catcactgca ggatagcggt
     5401 caatttacct cctcaaacgc aacgcaaacc tagaacggct tcggccaact attaatcaat
     5461 acatgccagg ttttactatg gatactaaaa agatattcaa gcacataccc tgggtgattc
     5521 tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca
     5581 gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca
     5641 gtctgtacat cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta
     5701 ttaacaacga cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact
     5761 tcgccgctat cgccggtgct ggtccgctgg tgggtccggt tctcgccgcg cagatgggct
     5821 acctgcctgg cacgctgtgg ctgctggcgg gggtcgtgct ggccggtgcg gttcaggact
     5881 ttatggtgct gtttatctcc tctcgccgta acggcgcatc tctcggtgag atgatcaaag
     5941 aagagatggg accagtaccg gggactatcg cgctgtttgg ctgtttctta atcatgatca
     6001 tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct ggccgaaagt ccgtgggggg
     6061 tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat gggtatctac atgcgcttta
     6121 ttcgtccggg gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg gttgcctcta
     6181 tctacttcgg tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag
     6241 ataccaccat tactttcgcg ctgattggct atgcgtttgt ctccgcactg ctaccagtgt
     6301 ggctgatcct cgcaccgcgc gactatctgg caaccttcct gaaaatcggc gttatcgtcg
     6361 gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcc atgacccagt
     6421 acattgacgg tactggcccg ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca
     6481 tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc ttccggtacg acgccgaaac
     6541 tgctggctaa tgaaaccgac gcgcgtttca tcggctacgg cgcaatgctg atggagtcct
     6601 tcgtggcgat tatggcgctg gttgctgcgt ccatcatcga accgggtctt tacttcgcga
     6661 tgaacacccc gcctgctggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg
     6721 agaacgcgcc gatcatcatg gcacagctga aagacgttac cgcacacgcg gcagcgaccg
     6781 tcagctcctg gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg
     6841 gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccaac gctggcggta ggtatcgctc
     6901 acgtgttcca caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc
     6961 tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg tacccgttct ggccgcttta
     7021 tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tctctggttg
     7081 ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg
     7141 tggtcgatcc gctgggcggc gttaagagcc tgtggccgct gttcggtatc tccaaccaga
     7201 tgctggcagc cgtggcgctg gtactgggca ccgttgtact gattaagatg aagcgcaccc
     7261 aatacatctg ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc
     7321 tgggcctgaa actgttcagc accaacccgc agatggaagg cttcttctac atggcaagcc
     7381 agtacaaaga gaagattgct aacggtactg acctgacggc gcagcagatt gccaacatga
     7441 accacatcgt tgtgaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg
     7501 tgtacagcat catcttctac ggtttcaaaa cctggctgaa ggtgcgtaac agcgacaaac
     7561 gtactgacaa agaaacaccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc
     7621 accactaacc ggttgcctta tccggcttac aaaatgtcta tcgtaggccg gataaggcgt
     7681 ttacgccgca tccggcaaat ccccagcccg gaccttccgg gctttacgca tatctggaag
     7741 gcaaaatgtt tggtaactta ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt
     7801 tgattggcat tccagactac gacaactacg tcgagcatat gaagaccaac catcccgaca
     7861 agccgtacat gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg
     7921 atggtaaagg cggtatgcgc tgttgttaat ggagagagca tgaacccgat tgcagttacc
     7981 ctactcaccg gttttttagg cgcaggcaaa accaccctgc tgcgccatat tcttaatgaa
     8041 caacacggct acaagattgc cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat
     8101 caactgattg gcgatcgcgc cacgcagatc aaaacgctga ccaacggctg catctgctgt
     8161 tcgcgctcca acgagctgga agacgcccta ctcgacctgc tggataatct cgacaagggc
     8221 aatattcagt tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc cggcccgatt
     8281 attcagacct ttttctccca tgagatttta tgccagcgtt acctgctgga tggcgtgatt
     8341 gcgctggtgg atgcggtaca tgccgatgag cagatgaacc agttcaccat cgcccagtca
     8401 caggttggct acgccgaccg tattctgctg actaaaaccg acgtcgcagg cgaagcagaa
     8461 aaactgcgtg aacgtctggc gcgcatcaac gcccgtgcac cggtctacac cgtcacccac
     8521 ggcgacatcg acctgggcct gctgttcaac accaacggtt ttatgctgga agagaacgtc
     8581 gtcagcacca agccacgttt tcactttatc gcggacaaac aaaacgatat ttcgtcgatt
     8641 gtggtggaac tggattaccc ggtagatatc agcgaagttt cccgcgtgat ggaaaacctg
     8701 ctgctggagt cggcggataa actactgcgt tacaaaggga tgctatggat tgacggcgaa
     8761 cctaaccgcc tgctgttcca gggcgtccaa cgcctctaca gcgccgactg ggaccgcccg
     8821 tggggcgatg aaaaaccgca tagtacgatg gtgtttatcg ggattcagct gccagaggaa
     8881 gagattcggg cagcgtttgc ggggttgagg aagtaatcgt gaaaggttga gtgtaaagca
     8941 acccaatgcc ccacttagca gtgaggggga aacagaacga cgtgctcccc tccgctaagt
     9001 tttattgcgc gtgaatacct ggatctggca aaaaattgcc gcgtgtggcg tcagcccatg
     9061 cttcgctacc agacgcagga atgtcgctgt aatcaatatg ttcgtcggat ttattaacca
     9121 aagctttaag ttcagcttct tgatgcacat caagtgtgta gagtgtgccc cgtttatgtt
     9181 taaccatgct catttatgac cagaaccttt actatataat ttataaagat gaattatttc
     9241 agccatccag gcacgttata ttctgagaca ttctcaatgg aatatgcatc tctcctgtca
     9301 gaacctttct tacatttcaa ctcatcaaaa c
//
LOCUS       DAZFRE010000154         9238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFRE010000154 DAZFRE010000000
VERSION     DAZFRE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.155"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(119..445)
                     /gene="ydbL"
                     /locus_tag="ACHKUI_004639"
     CDS             complement(119..445)
                     /gene="ydbL"
                     /locus_tag="ACHKUI_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6600685.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWMRKF"
     gene            complement(453..638)
                     /gene="ynbE"
                     /locus_tag="ACHKUI_004640"
     CDS             complement(453..638)
                     /gene="ynbE"
                     /locus_tag="ACHKUI_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6600686.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(635..3274)
                     /gene="ydbH"
                     /locus_tag="ACHKUI_004641"
     CDS             complement(635..3274)
                     /gene="ydbH"
                     /locus_tag="ACHKUI_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6600687.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLSVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLCDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            3482..4471
                     /gene="ldhA"
                     /locus_tag="ACHKUI_004642"
     CDS             3482..4471
                     /gene="ldhA"
                     /locus_tag="ACHKUI_004642"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6600688.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4582..5004
                     /gene="hslJ"
                     /locus_tag="ACHKUI_004643"
     CDS             4582..5004
                     /gene="hslJ"
                     /locus_tag="ACHKUI_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6600689.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFNGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5001..5267)
                     /gene="ydbJ"
                     /locus_tag="ACHKUI_004644"
     CDS             complement(5001..5267)
                     /gene="ydbJ"
                     /locus_tag="ACHKUI_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6600690.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5541..9065
                     /gene="nifJ"
                     /locus_tag="ACHKUI_004645"
     CDS             5541..9065
                     /gene="nifJ"
                     /locus_tag="ACHKUI_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6600691.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKHYDFLAQMAGKAEKSNTD"
BASE COUNT         2315 a         2417 c         2262 g         2244 t
ORIGIN      
        1 aataaataaa gcagagccga aacataactc agccttataa ttaaaagagc aaaatgtttc
       61 cggaaaatag attaatcgcc ttctcctgta gcgttatccc gatattgcgg ttttgctatt
      121 aaaattttcg catccattta ccgtttatcc cttgtacata ttccccgggt ttggcccgtt
      181 ctacgagctt ttgcccggcg agtttcgcga tatcatccac cgacacatta ttctgctttg
      241 ccagttgttg atagctcgct ttacgttcgg cattgatgtc ggttactaat ttctcagttt
      301 cagcatctgt tttcagcgca accagataac cataaaatgt ttcccctacc cgcccctgag
      361 ttctggcttc atccagagtc aatgccatta cattgctgct taccaagcca acaagaaatg
      421 cacaaagaag taatgttctt ttcatcatca cctcaaaaaa gatcgctacg ggtctcaagc
      481 agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt aacgttcata
      541 ttgatagtga tcggttcctt aggtgcagcg acttcaatgc gaggcgtaca gccaaccagc
      601 ataaaagatg acgtcaacgc agccagtaaa attttcattg tttatcctca cactcttttc
      661 cttgcggaca gtcatttccc ggcagacgcg cgttctgctc cagccatgcc tggagattat
      721 cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg taattaagat
      781 ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatattggcc tgcatggtta
      841 ataaaccgag attatctaaa ttaatttttg tcgatgaacg gctaatttcc atatagcgca
      901 accagttaat tgctgaaccc gcggtcatat tgtctttcac caccgcatct gcggtgtctt
      961 tatccagccg caaggtcatc ggcccgctat ttgccagcca gccgtctttc actatccatt
     1021 tttcgttgtt tagccataac ggcaatgcac cactaaatgc cccggacatg gcgaattgtt
     1081 tcggattgac ggcgctaacc agttcgctgg aggaaagatt attcagtcgc aacagtgccg
     1141 gatcatgttg cggcatacgt aattgtttca tcagcacgtt accgcctaac acatcgacgc
     1201 taacatcagt caacagcaat ggttcttctt cagtccacgg ataacgacct tgcaaatcag
     1261 ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac gtaacgggac
     1321 cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca aaatcgacac
     1381 cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg tgccctcccg
     1441 cgcggaatcc ttgttcaggc gcagctgaaa atgcaacctg agcatacagt tcaccatcgc
     1501 ataagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgtttag
     1561 gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc
     1621 caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg cgcccatcga
     1681 cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg
     1741 cgtccagtga cagagcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg
     1801 gcttgtcgag aattaatctc ggcttttcta ccgtcatcgt accgtattgt aactggtcga
     1861 aaccggtgga gagatcggtc agcgtaatcg tgctgtcatg ccactcacct tttcctgcga
     1921 catcccaggt ggcattcatc ggtgtaaaac tcccttttcc ccagtagcgc cactgccagc
     1981 ggccagcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca
     2041 gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca cgttgggtga
     2101 cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatca atgactcttc
     2161 cctttgaacg aagtaacgcg cctggctcaa aggtcagcgt tgggtcagac agacttccac
     2221 ttagctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca
     2281 gctgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa tttaacaccg
     2341 cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgcccgctg accagagcgt
     2401 tctcgagccc tgcctgccag ttgtcgactt tgacacccag tcggccactt aaaggaaaac
     2461 ctgcatacgg ccagctccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc
     2521 acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacaatcaat tgcccgctat
     2581 tttcctgcca gtccagctcg gcatccacca gtgaaggctc ctgcggtaag tttaacgtcg
     2641 cagtagcatg accacttacc ggaagtccat ccggcacgag cggcatagta aattccccca
     2701 ccagttttac cggcggctga ttttcaaacg cggcgacatc cagttcgctg actgtaagtt
     2761 gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga
     2821 tatcggaggt taatgcgaga gagagttttc cctgccattc ctgccaggga gaaaaaatca
     2881 gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg agggtttttg
     2941 gagcggctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa tcaagttcta
     3001 ccgtgccgac gctcaataac cagcggctgg gatgtgaaag gctggcgttg gtgatatgcg
     3061 caagctgaca atctcccacc agataacgga gatcggggat gattaaacct ttacgcgtaa
     3121 tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg cccgccagcg
     3181 tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata atcagtaata
     3241 acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct gatttagcga
     3301 aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg aaggttgcgc
     3361 ctacactaag catagttgtt gatgaatttt tcaatatcgc catatctttc aattatattt
     3421 gaaattttgt aaaatatttt tagtagctta aatgtgattc aacatcactg gagaaagtct
     3481 tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa
     3541 cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa
     3601 aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccgcccggt
     3661 gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ttgcgctgtg ctggtttcaa
     3721 taacgtcgac cttgacgcgg caaaagaact ggggctcaaa gtagtccgtg ttccagccta
     3781 tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat
     3841 tcaccgcgca tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggctt
     3901 tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct
     3961 gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcggc
     4021 ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt
     4081 tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg aagccgcctt
     4141 cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc
     4201 tcaggcggca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtgta
     4261 tgagaacgaa cgcgatctgt tctttgaaga taaatccaac gacgtgatcc aggatgacgt
     4321 attccgtcgc ttgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac
     4381 agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa
     4441 aggcgaaacc tgcccgaacg aactggttta atcttcccgc tcccctgcat tcctggggag
     4501 cttattcaga taatccccaa agacctttca tcctctattc ttaaaatagt cctgaatcag
     4561 aaacagtaat tgagaaccac aatgaagaaa gtagccgcgc ttgttgcgct aagcctgctg
     4621 atggcgggat gtgtaagtag tgacaaaatt gctgtaacgc cagaacagct acagcatcat
     4681 cgctttgtgc tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa tccgccagaa
     4741 atcagctttg gtgaaaagat gatgatttcc ggcagcatgt gtaaccgctt taacggtgaa
     4801 ggcaaactgt ctaatggtga actgaccgcc aaagggctgg caatgacccg tatgatgtgc
     4861 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     4921 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     4981 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     5041 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttggcgg
     5101 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     5161 gcttttaaac ctggcgctac gtgcgccgca gtcgcctgct gaacaggttc actactacac
     5221 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctt ttcttcctct
     5281 gatcttcaag ccaaacaaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     5341 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5401 tgatcaaaac attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5461 catttgctaa aattgaacga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5521 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     5581 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     5641 aacaggctga tgcatgggcc ggaaacggct tgaaaaacgt ttggggagac acaccacgcg
     5701 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggtgct ttgcaaactg
     5761 gtgccctctc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccaacgctgt
     5821 acaaactggc aggcgaactc acaccgtttg tcctgcatgt tgcggcacgt accgttgcca
     5881 cacatgcact ctctattttt ggcgatcatt ccgacgttat ggcggtgcgc cagacgggtt
     5941 gcgcgatgtt gtgtgcagca aacgtccagg aggcgcaaga ctttgcactc atttcgcaca
     6001 tcgcgacgct gaaaagccgc gtgccattta ttcatttctt tgatggtttc cgcacatccc
     6061 acgaaatcaa taaaattgtc ccgctggccg atgacacgat tcttgaactg atgccgcagg
     6121 cagaaattga tgctcatcgc gcccgggcac tcaacccgga acatccggtg atccgcggta
     6181 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaaccca tggtacaacg
     6241 cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca ggtcgtcagt
     6301 atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     6361 ctgccattgg caccagtgaa gaagtggttg atgaattgct gacccgtggc gaaaaagtcg
     6421 gcgtgctgaa agttcgcctg taccgcccct tctccgccaa acacttgctg caagctctgc
     6481 cgggatccgt gcgcaacgtg gcggtactgg acagaaccaa agagccaggt gcgcaagcgg
     6541 aacccctgta cctggatgtg atgaccgcac tggcagaagc ctttaataat ggcgagcgcg
     6601 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     6661 actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg cgctttacgg
     6721 ttggtattta cgatgatgta accaatctgt cactgccgtt gccggaaaac accctaccaa
     6781 actcggcgaa actggaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcga
     6841 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     6901 tttacgattc caaaaaggcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     6961 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     7021 ttatcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc ctgctcaaca
     7081 cgccgtacag cgcagctgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgctaa
     7141 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     7201 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg cagattctgc
     7261 ctggcgatag tgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     7321 gccaggatct ggtggaacgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7381 aagttccact gcagccagtt aatccgcata gcgccaatcg accgccagtg gtttccgatg
     7441 ccgctcctga tttcgtgaaa accgtaaccg ctgcgatgct cgccgggctt ggcgacgccc
     7501 tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccacg cgctgggaaa
     7561 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     7621 actgtgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg cctgaagcga
     7681 tggaaaacgc ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat atgcgcgggc
     7741 agaaatatgt cttgcaggtg gctccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     7801 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtcgcgcc
     7861 tggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     7921 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     7981 aatattccgg tgcttgctcc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     8041 tctatggcga ccggatgttg atcgctaatg ccactggctg ttcttcaata tacggcggta
     8101 acctgccttc aacaccatat accaccgatg ccaatggtcg tggaccggca tgggcgaact
     8161 cgctgtttga agataacgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     8221 gtgtccgcgt gctgcgtctg ctcgatcaat ttgccgataa aatcccggca gaattactga
     8281 ctgcgttgaa atcagacgcc acgccagagg ttcgtcgtga acaggttgca gctttacgcc
     8341 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     8401 aaaaatcaat ctggctgatt ggtggcgatg gctgggctta cgatatcggc tttggcggtc
     8461 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8521 attccaacac cggtggtcag gcatcgaaag cgacaccgct gggtgcagta actaagtttg
     8581 gcgagcatgg taaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     8641 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     8701 aggaagcgga agcgtatccg gggccatcgc tgatcatcgc ttatagccca tgtgaagagc
     8761 atggttacga tctggcactc agccacgatc agatgcgcca actcactgct accggcttct
     8821 ggccgttata tcgcttcgat cctcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     8881 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcag cgtttccgtc
     8941 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     9001 tgcaaaagca ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa agcaacaccg
     9061 attaaatgct ctggataagg atcatccaat tctaaaaaaa agcccggacg actgttcggg
     9121 cttgtcttct tttgtgttgg aaaatcagta gcaatgcaat ggcccaacag aaaataaaat
     9181 ctgtgacaat aaaggcatat aacccgcgca gaataacgta tagaaaatta aaagtata
//
LOCUS       DAZFRE010000155         9164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFRE010000155 DAZFRE010000000
VERSION     DAZFRE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.156"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..189
                     /locus_tag="ACHKUI_004646"
     CDS             <1..189
                     /locus_tag="ACHKUI_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO6600692.1"
                     /translation="PSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLTDYVP
                     MMPHSHQSDTRGLLKSVS"
     gene            171..599
                     /locus_tag="ACHKUI_004647"
                     /pseudo
     CDS             171..599
                     /locus_tag="ACHKUI_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            668..2191
                     /locus_tag="ACHKUI_004648"
     CDS             668..2191
                     /locus_tag="ACHKUI_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6600693.1"
                     /translation="MKRAISYIRFSSEKQSRGDSLRRQSKMVNDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDVDQASELLR
                     NILRSGVDVVTLSDGEHYTKESLKDPLALIKSILIMQRSHEESLRKSERIQAAWDAKK
                     EAAKAGIKIYKRCPAWLKLNDDRKTFSVIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQTLNQYKAGKFGQTSVQDLLKNRSVIGYFTPKGCEEIAGYFPPIIPETDFYKVQQ
                     LSYSRYGRKPASDKPLSVNLFKGVIRCSECGHALIITGFSDTRNGIYRCPLRNENRCS
                     AKGLSRKQTDGTLLALLSRIDRFDSDMTNTIDTLKLHREHISSNIEKLVQLSLEIEDL
                     SAITSKLKQLKGELSQVDADIEKETQRLKSIDVGCLSDCDLTTKAGRIEAQLIIKSAV
                     KEVILNTAKRRCKVTFHNSKSITLPITETPSEDVTEAIQSLSEVTERGLIDVDEVII"
     gene            complement(2206..2979)
                     /locus_tag="ACHKUI_004649"
     CDS             complement(2206..2979)
                     /locus_tag="ACHKUI_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO6600694.1"
                     /translation="MIYSNQIKYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGNGMLLCQELYNRSTSELTNEQLQARINCGKSVKV
                     ENGKSTFSDAANAQASKVLNNAGTGSTLKKFSVNPNKRF"
     gene            complement(3137..5185)
                     /locus_tag="ACHKUI_004650"
     CDS             complement(3137..5185)
                     /locus_tag="ACHKUI_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6600695.1"
                     /translation="MAQFKVDSFIVELGFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQ
                     PRISGNRAITAAYSANMSKLKGLNPIVEKYIKSQFYALSSKSKTLGNEAFNRELAKLN
                     QNLRETLSKFNKTTSKNNHSENASNGLDVLASSAIKAGTAIYTFQSALAAYQRVMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFRSMIGYSRLMGRSEEEIKRALTALSQMAGKGQVMAEELKGQLAEAVPGMVQVF
                     AKATGKTEQELFDAMKKGALKSADTLQKVTQELNRQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSESGLQDFTRSLTTLLNALGGSGKSLGESLGSLMTDMSHGVDSLA
                     DISYKVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSI
                     YGLMTAITRLGQVADTTADNVNRKNGKPSRLNLGNMLLAGSALYAGYERLASTPERME
                     AQAKLPELAKESGLKPVFTMDDVKGWFNSAAMNLANFQHPELITMRQDAALTNTDIQS
                     LRDEISALSKRIQEPVKVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD
                     "
     gene            complement(5369..5578)
                     /locus_tag="ACHKUI_004651"
     CDS             complement(5369..5578)
                     /locus_tag="ACHKUI_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600696.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKTKHQIITSRKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(5602..6309)
                     /locus_tag="ACHKUI_004652"
     CDS             complement(5602..6309)
                     /locus_tag="ACHKUI_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6600697.1"
                     /translation="MENLYINGKKIDSFGDWSPQTEHPAIAIQRKEHDERIALEQELK
                     MSPKEIIFVTPEPQEMPDVCKTESLLELEREYYPLLKAQRIKLDIAYDAVKNFEETSK
                     PSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSGQ
                     FILTTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDSMKAIISDYETQKKVAMQ
                     ADIEQLTKISQKYSKAL"
     gene            complement(6882..7187)
                     /locus_tag="ACHKUI_004653"
     CDS             complement(6882..7187)
                     /locus_tag="ACHKUI_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600698.1"
                     /translation="MNYSMQVMVDQETMNWLNDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDFVHKLEVTDPDYKRFITSECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(7334..7549)
                     /locus_tag="ACHKUI_004654"
     CDS             complement(7334..7549)
                     /locus_tag="ACHKUI_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600699.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKAPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(7770..8060)
                     /locus_tag="ACHKUI_004655"
     CDS             complement(7770..8060)
                     /locus_tag="ACHKUI_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600700.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGELLKESVSLTP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLEV"
     gene            8197..8382
                     /locus_tag="ACHKUI_004656"
     CDS             8197..8382
                     /locus_tag="ACHKUI_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600701.1"
                     /translation="MGFLETIAESHRFASLGCFVFLVLLVYQLLKMIIAKSTRFNFSI
                     KLFKYEITITIDDGKTN"
     gene            complement(8379..8669)
                     /locus_tag="ACHKUI_004657"
     CDS             complement(8379..8669)
                     /locus_tag="ACHKUI_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600702.1"
                     /translation="MKEFHGEKRYKDYLLKRYSISREGHLMKDTYGEVYRIRPKKEGR
                     DYYFVDGVTDLKIDALRFAVLYHYDIWDSVHQLRLKDGDPGNLRATNIITKR"
     gene            complement(8669..>9164)
                     /locus_tag="ACHKUI_004658"
     CDS             complement(8669..>9164)
                     /locus_tag="ACHKUI_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6600703.1"
                     /translation="INNNKEDDFEEESLKKGASLCDSHECDSLDIEEAIKETVKTSDL
                     DDLDSEATKEAVKKEDKISDVNEVSDQQDTPCRLESTDDELPDVDLDDEEDPEEELKR
                     EQRKAEIKERFRKAKLKNRIDELDDFDDSDDDDFSFTPKKKTHTEPKKEKVKVRRIYA
                     GGMR"
BASE COUNT         2568 a         1929 c         1723 g         2944 t
ORIGIN      
        1 ccaagcttca ttgccagcca gatgggccat gcaagtgccc agatggtatt caatgtatac
       61 ggagcatgga tgactgacag caatgcagaa cagatcgcaa tgctgaatca gaagctgaca
      121 gattatgtcc caatgatgcc ccatagtcac caaagtgaca ccagaggctt attaaaatca
      181 gtaagttaat ccttaatacc cgtcatgtta actgcgtgga gggtaacacc acgctttatg
      241 ccctgccgaa acccgaggtt gtcctgcgct ggcgtgagca gaccacagat gacttccgct
      301 tctgttttaa gtttccggcg accatttcgc atcaggcagc attacggcat tgcgatgatt
      361 tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattgga caatactggc
      421 tgcaactgcc tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgatt
      481 ctcttcccgg tgaatttaat tatggggtgg aagtccgcca tccacagttt ttcgccaaag
      541 gggaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat cgggtgattt
      601 ttattagaca cgtttaaata caccatctaa aacgatacaa tctagggcat cagttttgag
      661 gtgaacaatg aaacgtgcca tttcttacat caggttttca tctgagaagc aatcccgagg
      721 tgattcatta cgccgacagt ctaagatggt taatgactgg cttaaggctc atcctgagta
      781 ctaccttgac caagatttat cttttcgtga tttgggggtg agtgggttca ctggtaagca
      841 cttgaaaggt ggcttaggtg actttttaac cgctatcgag aaagggcttg ttaaagctgg
      901 tgatacgctc ttgatagaat ctcttgaccg tctgtcacgt caagatgtag accaagcaag
      961 tgagctactc agaaacattc ttcgctctgg tgtagatgtg gtcaccttgt ccgatggtga
     1021 acattacaca aaggaaagct taaaagaccc gttagctctg attaaaagca ttttgataat
     1081 gcagcggtcg catgaggaga gtttgcgtaa atctgaacgt atccaagctg catgggatgc
     1141 aaagaaagaa gctgctaaag ccggtatcaa gatttataag cgttgtcctg catggcttaa
     1201 gcttaatgat gaccgtaaga ccttctctgt gatacctgag aaggtagaaa ctgtgaagag
     1261 gtgctttcaa ttacggttag agggtaagtc tatttgggag attgtcagga ctcttaatga
     1321 tgaaggtcac cagacactta accagtacaa agcaggtaag tttggtcaaa cctcagtgca
     1381 agaccttctg aaaaatcgct ctgtaattgg ttacttcaca cctaaaggct gtgaggagat
     1441 agctggttat ttccccccta tcatccctga gactgatttc tacaaagtcc agcaactatc
     1501 atattctcgt tatggaagaa agcctgcttc agataaacca ttgagtgtga atcttttcaa
     1561 aggtgttatc cgttgttctg aatgtggtca tgctttgatt atcacaggct ttagcgatac
     1621 aagaaacggg atttatcgtt gtcctcttcg taatgaaaac cgttgtagtg ctaaaggact
     1681 ttcacgtaag caaaccgatg gtacattgct tgccctgcta tcccgtatag accgatttga
     1741 tagcgatatg acgaatacca tagacacact gaagctacac cgtgaacaca tctcaagcaa
     1801 tattgaaaag ctggtacagc tatcacttga aattgaagac ttgtccgcta ttaccagcaa
     1861 actaaaacag ttaaaaggtg agctatcaca ggttgatgcg gatatcgaga aggagacaca
     1921 acgccttaaa tctattgatg ttggttgttt gtctgattgt gacttgacta caaaggcggg
     1981 aaggattgaa gcacaactga taatcaaaag tgctgttaaa gaggtcattt taaatactgc
     2041 taaacgtcgc tgtaaagtaa cttttcacaa tagtaagtca atcaccctac caataacaga
     2101 aaccccctca gaggacgtta cagaggctat acagtcgctt tctgaagtca ctgagagggg
     2161 gttgatagat gttgatgagg ttatcatata gttgagattc tatcgtcaga atcgcttgtt
     2221 aggattcaca gaaaatttct tcaaagtact acctgtacca gcattattaa gcacctttga
     2281 agcttgcgca ttagcggcat ctgagaaggt gctttttcca ttctcaactt tgacactttt
     2341 accacaattg atacgggctt gaagttgctc attagtgagt tcactcgtag agcgattata
     2401 tagctcctga cacagcaaca taccattacc tagacttgtc gtagtgttac cagtgttctt
     2461 cttagaagtt gcaccaccat cattcgtggt agcagtcttt ccagttgcag cagaaatatc
     2521 agtctgacca acaatagctt tgaagttaat ttcctgaaaa gttagctgga ttcttagacc
     2581 gtttgaaatc cccacatctt tagaggcttt aaaactcgta ataatggaat cgtcaatttt
     2641 aattccgtct ttacagatga ccgaaataat ctgtttctgg tcacgccagc tttcaagagt
     2701 gtcgatgaag ttttctacta attgaccctg acgagttaat aataagcttc cttcatagcc
     2761 aactacaacg acaccactaa tagtgattgt tctgggtgct ctttgcacat tatctgtaac
     2821 ggtttgtcct gattgcatgt tctgtgtagt cacctgcata ggactatcaa attccatgtt
     2881 ttcagttgct gataaggtta agaaggcatc tacattatct cttaagtgga aatagatgcc
     2941 gtctttgcca ctatatttga tttggttaga gtaaatcatt aaattcccct ttgatttcaa
     3001 aggcttagtg tattttaatc atttttaacc aagcctttga tatataagtg ttttctctgt
     3061 taagcgtgtt gtacgtaaat tctatttttc agtgtgggta ttagctttac agatttcacg
     3121 tagcattcga ctattatcaa tcatcttctg ggaatgatga acttaatagc gtagcttctg
     3181 cgtattggtc gtagatatta ctgctaaagg tcataaagct ggtttcatct ggtttaatgg
     3241 caacttcacc accaagtgaa actttcacag gctcctgaat acgtttgctc aaagcactaa
     3301 tttcatctct caggctttgg atatccgtat tcgttaatgc cgcatcttgt ctcatggtga
     3361 ttaattctgg atgctggaag ttagcaagat tcatagctgc actattaaac caacctttca
     3421 catcgtccat agtaaatact ggcttaagtc cagattcttt agctaactct ggtaatttag
     3481 cttgtgcttc cattcgttca ggtgtagagg ctagtctttc ataacctgca tagagagcgc
     3541 taccagctaa tagcatattc ccaagattca gtcttgatgg tttcccattc tttctattca
     3601 cgttatccgc tgtagtatca gcaacctgac ctaatcttgt gatagcagtc ataagcccgt
     3661 aaatacttct ggttacacca acaactttat ttacagcaat aacaccagcc aagactttta
     3721 aactattaat caagccagaa cctaaagcat caacaacttt cttttgttca tcggttaaac
     3781 cacgataagc taatgtcacc tcatcaaaga acgctcgcac cttgtaacta atatcggcca
     3841 gactatcgac accatgtgac atatcagtca tcaagctacc aagagattca ccaagacttt
     3901 taccacttcc accaagagcg tttaaaagcg ttgttaagct tcttgtaaaa tcttgcagac
     3961 cactttcaga acctctgaag atactatcaa gcgtggtatt ccaagagttt ttcaggttcc
     4021 ctaattgtgc ctgtgtactt tctgaaatag ctttccagcc acctttagcg gtgatttgtc
     4081 tattcagttc ctgagtgacc ttctgaagag tatcggcaga ttttaaagcc cccttcttca
     4141 tggcatcaaa taattcctgt tcagtctttc cggttgcttt agcgaatacc tgaaccatac
     4201 caggaacagc ttcagctagt tgccccttaa gctcttcagc cataacctga cccttaccag
     4261 ccatctgtga taatgctgtc agtgctcgct taatctcttc ttcacttcga cccatcagac
     4321 gagaatagcc aatcatactt ctaaaaagtt cttcagaacc ttgaataccc atagactctt
     4381 tagctgaagc accgaaacca gcaaactgtg attgcagttc agcgatatct aaacccgttg
     4441 tttgagctaa tgactttgtg aactctttta cctgacctgc atctttatca aatacgaaat
     4501 caattgaacg ttcagcactt gcacgctgta aaccaatttc cattactcgc tgataagctg
     4561 ccaaagcact ttgaaaagtg taaatagccg tacccgcttt aattgcactt gaagctagta
     4621 catctaaacc atttgaagca ttttcactgt gattattttt gctggtggtc ttgttaaact
     4681 tagaaagtgt ttcacgtaga ttttgattta actttgctaa ctcacgatta aaagcttcgt
     4741 ttccaagtgt cttggattta ctacttaatg cgtaaaactg actcttaatg tatttctcga
     4801 caattgggtt aagacctttc agcttagaca tattcgctga ataagccgct gtgattgctc
     4861 tgttgccgct aattcgtggc tgtcttggct gtctaagtgg ctttaaagta tcctcaattt
     4921 ccgttttaat cttaaacgct ctagtgtttt taagacgctg ctcaatcttg tcaaaggtct
     4981 tattaaactt cctttcaagt tgccctaaag aacttgtcag attgctgtca agttgcttgg
     5041 tatcgacctt aaacgctcta ttcaaattac gttcaattct ttgtgctgac tgcaaagctg
     5101 ccttttcaac tctttgcaag cccttaataa cgttctcgct gaagcctagc tcaacgataa
     5161 aactatcaac tttaaattgt gccattgttc atatttcacc tctgttaagc gtgagctacg
     5221 taaaaaataa tttttggtgg gtatatctgt tcagaagatt tcacgtagca cacatacaaa
     5281 aaagggagaa actaccgaat aatatcgata atctctcccc gtgttataga ttcgatagaa
     5341 cctatctcat acacccaacc aatctttttt aatgcctgac aagataatgt agttccgttt
     5401 cacctataat ttgagcatcg gaagggatac ggtcgataca ccactttgga atcttgcgtg
     5461 tttttggttt gcgtgaggtg ataatttgat gtttggtttt aattcttaag gctggcttag
     5521 tttgtaactt taagttttca caaacagacc gaatagtctc taaacgttct tcaaacatta
     5581 atcacctctt ttaattcata atcaaagtgc tttgctgtat ttctgagaaa tcttcgttaa
     5641 ctgctcaata tcagcctgca tagcaacctt cttctgcgtt tcatagtcac taataatcgc
     5701 tttcatgcta tcaagttctt ttttaagctt ttgctttaac ttatccagtt taccggaata
     5761 atgcttagtc aaattagctt cagctaactg aagaatctct tcatctgttt tatcagtcgt
     5821 caaaataaat tgaccagaac cccttaatgc ttgtctgaca tcaattaacc ggaacccttc
     5881 agggatactt tgaagaactt taggaacgtc ttcaatcgct gtaccaaaac catcattaaa
     5941 gtttccttca taatagataa acgggtttga cttaatctcg taagcaatat catattctga
     6001 aggtttcgag gtttcttcga agttcttaac cgcatcgtaa gcaatatcaa gtttaattct
     6061 ttgtgctttc agtagtgggt aatattcacg ctcaagctca agtaatgatt cagttttgca
     6121 tacatcaggc atctcttgag gttcaggagt cacaaagatg atttcctttg gtgacatctt
     6181 taactcttgc tcaagtgcaa ttctttcatc gtgctctttg cgctgaatag cgatagctgg
     6241 atgttcagtc tgtggtgacc agtcaccgaa gctatcaatc tttttaccgt taatatatag
     6301 attttccatt tcattttcct cttctaagta atattcagtt tttaaaaagc acacctgaca
     6361 gcctatctaa acttagaaga gacttaaaag gcggtttgcc aagtatgctt tttaaaagct
     6421 gaaaaagccc taaacatagg tgattcggat attcatggaa tcatccttat ctgtgaaggg
     6481 ctttggaggt ttgctttata acgttaacga agtttcagga aaaactttct ttaaaagttc
     6541 tgtttaaaaa ctcttaaaga aagccctaat catgattagg gcaaagctgt aaagggttca
     6601 atgaatgaat gattaagcac ttttcgagat gacctctcag gttatataag aggccatgta
     6661 gcgaaaagca ttgcaccttt cagaataact atccctagat gtagatggaa ttgttgaaag
     6721 aaataagaga gcaattctct ctataattaa aattatgtca tgcttaaggt tatttgtcaa
     6781 cagcttttta acagattggt aacattaatt cttatttacc gtattaccta cccagcaacc
     6841 aacaaatagc cctaaaaccg tccagagaat aacgagtgtc attacatcac ctcactgtaa
     6901 gggtttaatt cattctgata ggtcaagaat acattcagct tcaataattg ctccgggctt
     6961 aaatcaagtc ggttgcattc gcttgtgata aatctcttgt agtctgggtc tgttacttcc
     7021 agtttatgaa caaagtcacc aagtttaatt tcgttgatta tatgcttgtc aatatacgtt
     7081 gacgcttcaa aacaatgcat gaaatagata ttaaagcccg acctgttcag gtcattaagc
     7141 caattcatag tttcttggtc aaccataact tgcattgaat aattcatttt taaaatcctt
     7201 cctgtatcgc attctaagac gttttaaccc cttttttaga ccacttaagg cgattcccta
     7261 agttaccctg attaggggct taaaatcgct tagaattcgt ctggtaaagc ctgagagata
     7321 tgttttactg tttttagctc aagtctgaga ctaatagccc catctggtga ccgtagtctt
     7381 ttaaccagct ttgcttaacc ggagccttca gagaatagtt aggggctgac aagtgaagat
     7441 agatatcatc tttgccgtgg atgcttcccc atccttcgta taactcaagc aagttttcaa
     7501 aataacgaaa tgctttatta gtctgaagtt gtactgtgat agttttcatc aagcacccca
     7561 ttcagaaata agcttaattc cctgctcaag ctgttcaggg tcgttatagt cgagtattac
     7621 cagtggcttt aattcgtgtt taagctggtg gatttgagag ggttttaatc gtttgtttgc
     7681 tttgagggtc acatcttcaa attcagcatc tgctaaattc tgtctgatta ggtggctgcc
     7741 aagtgtggaa tattggaacc tgtaacacat taaacctcca atttatccgc cagttgatgg
     7801 accttagcac tcaacacctg aatcattgag caacctgcta ctgcggactg tgctttgagt
     7861 agttccagtt ggcccgttgt gattctgatg gccttagaac cctctgcaac acccttaacg
     7921 ttatcgaaag gggtcaggga taccgactct tttaaaagct ctccaacaag cgctgtgtgc
     7981 ttcatatcgg gatatttagc tttgattgct gccagtcgtt caaatgcctc tggcaaaagg
     8041 cgtgtaactg tgtgagtcat tgtaaaatct ccttaaatct cttactcata ctacaatctt
     8101 aacatatatt taacatattg tcaacaactc atttacatgt taatatggtt ataggggctg
     8161 atacaggtca ttccctttga taaataaggg gagttaatgg gttttcttga aacaattgct
     8221 gaatctcaca gattcgcaag cttaggttgc tttgtgtttc tggtgcttct ggtgtatcag
     8281 cttttaaaaa tgattatcgc caaaagtaca cgattcaatt tcagcattaa gcttttcaaa
     8341 tatgaaatca caattacgat tgatgatggt aaaacaaact aacgttttgt gatgatattg
     8401 gtggccctca gattaccggg gtcaccgtct tttaatcgta gctggtggac tgaatcccag
     8461 atatcataat gatacagcac tgcaaacctc aaagcgtcaa tctttaaatc tgtcacacca
     8521 tcgacgaaat aataatccct cccttctttc ttcggtcgaa tccggtaaac ctccccataa
     8581 gtatccttca taaggtgacc ctcacgagaa atgctgtaac gctttaacag gtaatctttg
     8641 tatcgttttt caccgtgaaa ttctttcatt agcgcatacc tccagcataa atacgtctta
     8701 ctttcacttt ttctttcttc ggttcagtgt gggttttctt ttttggtgtg aaagaaaagt
     8761 catcatcgtc actatcatca aagtcatcaa gttcatcaat tctgttcttt agcttagcct
     8821 ttctgaatct ttctttaatt tctgctttac gctgttcacg tttcaattcc tcttcagggt
     8881 cttcttcatc atcaaggtca acatccggta attcatcatc agttgattcg aggcggcaag
     8941 gtgtatcttg ctggtcacta acttcattca catcagagat tttatcttcc tttttaacag
     9001 cctctttagt agcttcacta tcaaggtcat ctaagtcaga tgttttaaca gtctctttaa
     9061 tagcctcttc aatatcaaga gaatcacatt catgtgaatc acagagcgaa gctcccttct
     9121 taagagattc ttcttcaaag tcatcttctt tattgttatt aata
//
LOCUS       DAZFRE010000156         9052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFRE010000156 DAZFRE010000000
VERSION     DAZFRE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.157"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            114..656
                     /gene="ecpR"
                     /locus_tag="ACHKUI_004659"
                     /gene_synonym="matA"
     CDS             114..656
                     /gene="ecpR"
                     /locus_tag="ACHKUI_004659"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6600704.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            731..1318
                     /gene="ecpA"
                     /locus_tag="ACHKUI_004660"
                     /gene_synonym="matB"
     CDS             731..1318
                     /gene="ecpA"
                     /locus_tag="ACHKUI_004660"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6600705.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1375..2043
                     /gene="ecpB"
                     /locus_tag="ACHKUI_004661"
                     /gene_synonym="yagY"
     CDS             1375..2043
                     /gene="ecpB"
                     /locus_tag="ACHKUI_004661"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6600706.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2069..4594
                     /gene="ecpC"
                     /locus_tag="ACHKUI_004662"
                     /gene_synonym="yagX"
     CDS             2069..4594
                     /gene="ecpC"
                     /locus_tag="ACHKUI_004662"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6600707.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGEAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSSTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4584..6227
                     /gene="ecpD"
                     /locus_tag="ACHKUI_004663"
     CDS             4584..6227
                     /gene="ecpD"
                     /locus_tag="ACHKUI_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6600708.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6196..6906
                     /gene="ecpE"
                     /locus_tag="ACHKUI_004664"
     CDS             6196..6906
                     /gene="ecpE"
                     /locus_tag="ACHKUI_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6600709.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7219..7548
                     /gene="ykgJ"
                     /locus_tag="ACHKUI_004665"
     CDS             7219..7548
                     /gene="ykgJ"
                     /locus_tag="ACHKUI_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6600710.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7560..7726)
                     /locus_tag="ACHKUI_004666"
                     /pseudo
     CDS             complement(7560..7726)
                     /locus_tag="ACHKUI_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7797..8411)
                     /gene="yagU"
                     /locus_tag="ACHKUI_004667"
     CDS             complement(7797..8411)
                     /gene="yagU"
                     /locus_tag="ACHKUI_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6600711.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8626..8811)
                     /locus_tag="ACHKUI_004668"
     CDS             complement(8626..8811)
                     /locus_tag="ACHKUI_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600712.1"
                     /translation="MASGLICHGGFEPHAWGVANLMDVAVLVVAYLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8860..9018
                     /locus_tag="ACHKUI_004669"
                     /pseudo
     CDS             8860..9018
                     /locus_tag="ACHKUI_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2435 a         2295 c         2239 g         2083 t
ORIGIN      
        1 cttatttact tccgatttac taaagaaact gaatgtacct gtaaaaatta caggtttgga
       61 aagtagtgac atggcaaaat gattacagca gggactatga ggttaaaaac catatggaat
      121 gtcaaaaccg ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac
      181 tatctgaact gattgtggat atcgacagat taatttatct atcgctggag aaaatcagaa
      241 aagatttcgt gtttatcaat ctcaatacgg attctttaac tgagtttata aaccgtgata
      301 atgagtggtt atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag
      361 aagccttagc aaattattgg tattacaaca gcaatattag gggcgtggta tacgctggac
      421 tgagtcgtga tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag
      481 atattaagaa agataaaatc actgaccggg aaatggaaat tatccgcatg acggctcagg
      541 gaatgctgcc taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc
      601 atcggcggaa tgcagaggcc aagctgtact caaaattata taagttggtt cagtaaactc
      661 caggcaagtt agttttaaaa aatgactcac cgggacttca tgtcctcaat tcaactcggg
      721 aagaaaagca atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggtac
      781 aggtgtagcg caggctgctg acgtaacagc tcaggctgta gcgacctggt cagcaacagc
      841 caaaaaagac accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta
      901 tgccgaaggc attaaaggtt ttaactcaca aaaaggtcta tttgacgtgg ctatcgaggg
      961 tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca ccttaaccca
     1021 gttggatacc tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga
     1081 aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag
     1141 cccactggct aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacttt
     1201 ctccatcatc agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc
     1261 ggaaggcatc tggagcggcg acgttagcgt acagttcgac gctacctgga ccagttaatc
     1321 tctctgatgt accagcaggg gtacccccct gctttattcc tggacggaat tgttatgaaa
     1381 aagcaccttc tgcctctcgc tctgctgttt tccggaatat caccggccca ggcgctggat
     1441 gtcggcgata tatcatcgtt tatgaacagt gacagcagca cgctgagcaa aacgatccaa
     1501 aacagtaccg acagtggccg cctcatcaat atccgtctcg aacggctctc ttcaccgctt
     1561 gacgacgggc aggttatcgc aatggacaag ccggatgagt tgctactcac tcccgccagc
     1621 ttgctgctac ccgcccaagc cagcgaagtg atccgcttct tctataaggg accagcagat
     1681 gaaaaagagc gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc
     1741 gataatgcca accgtagcgc tgtggccact gcttccgccc gcatcggcac cattctggtc
     1801 gtcgcacccc gccaggcgaa ctaccacttt cagtacgcca acggctccct gacaaataca
     1861 ggaaatgcga cgctgcggat cctcgcctac ggcccctgcc tgaaagccgc caatggtaag
     1921 gagtgtaaag agaattacta cctgatgccg ggcaagtcgc gtcgttttac ccgcgtggac
     1981 acggcggata acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa
     2041 tagatagctg tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa
     2101 agcccagttt gccttcggca tggtcttttt gttcgttcag cccgatgcca gcgctgctga
     2161 cataagtgcg cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgcttca
     2221 ggacggcatg agcgtcccgc tctatattca tctcgccggt agccagggtc gccaggacga
     2281 tcagcgaatc ggcagcgctt ttatctggtt ggatgatgga cagttacgca tccggaaaat
     2341 acagctggaa gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcaactgat
     2401 gactctggcc aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca
     2461 gttggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg
     2521 caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaata ccctcagcag
     2581 taatctgagc tataacttcg gcgtctataa caaccagttg cgtaacggcg ggagcaacac
     2641 ctccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcacg tggtgctcga
     2701 cggctcactg tacgggattg gtagcggtca acaggacagt gaattatata aagcgatgta
     2761 tgaacgcgat tttgctggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca
     2821 gtccttaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa
     2881 ccaggccagc tccaccatct tcgacagcag ccagtcggcc acgccagtga tcgccttttt
     2941 accggcggcg ggcgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt
     3001 caccatgggc aatcatgaag tggatacccg gggtctaccg tacggtattt acgatgtgga
     3061 agttgaggta atcgttaacg gtcgcgtgat cagcaaacgc acccagcggg tcaataagct
     3121 gtttagccgg ggacgtggcg tcggtgcacc gctggcgtgg cagatatggg gcggtagctt
     3181 tcatatggat cgctggtcgg aaaacgggaa aaagacgcga ccagccaaag agagttggct
     3241 ggcaggtgcc tcgacctccg gctcactgag tacgtttagc tgggcggcaa cgggatatgg
     3301 atacgataat caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg aggcgatcaa
     3361 cgttaacctg caaaacatgc tggccagtga cagctcatgg agcaacatcg ccagcatcag
     3421 cgccactcta cctggaggct tcagttcgct gtgggttaac caggaaaaaa cccgcattgg
     3481 caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa
     3541 ctcactgtgg tcgaaactgg gtacgttcag catcagctac aatgatgacc gccgctacaa
     3601 cagccattat tacacggcag attactatca aagtgtctac agcagtacct ttggttcgct
     3661 tggcctgcgg gccggtattc agcgctataa caacggcgac agcagcgcca atacagggaa
     3721 atatatcgct ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgaccca
     3781 tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aaggaaccat
     3841 tcgcactgtt ggtgccaatc tgtcacgagc catctccggc gataccggtg atgacaaaac
     3901 cctcagcggt ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt
     3961 caatagcgcg gcggacggct acatcaatac caatttgacc gccaacggca gcgtcggctg
     4021 gcagggtaaa aacatcgctg ccagcgggcg gaccgatggc aacgctgggg tgatattcga
     4081 cactggcctg gagaacgatg gtcagatcag cgccaaaatc aacgggcgga ttttcccgct
     4141 taacggcaag cgtaactatc tcccgctctc tccctatgga agatatgagg tggagttgca
     4201 gaacagcaaa aactcactcg acagttacga tatcgtcagc ggtcgcaaaa gtcatctgac
     4261 tctctatcca ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc
     4321 cggtcgtatc cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat
     4381 cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg gacgtggata agaaataccc
     4441 cactatcgat tttcgctaca gtggcaataa aacctgcgaa gtggcactgg aactcaacca
     4501 ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcggc
     4561 ggtgacgcag acaggagaag agaatgagag ttaacctact gatagcgatg ataatctttg
     4621 cgctaatctg gcctgcaacc gcgctcagag cggcagtgag caaaacaacc tgggcggatg
     4681 ctccggcacg cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc
     4741 ctggcggggc gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca
     4801 atggttcagg aaccatctat cagcaaagcc tcggctacat tgataacggt tacaacaccg
     4861 gcctttatac caactggaag tttgatatgt ggctggaaaa ttcaccagtt tcatctcctt
     4921 taactggctt gcgctgcatc aactggtacg cagggtgtaa tatgaccacc agtcttatcc
     4981 tgccgcaaac caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga
     5041 aatggatgca cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg
     5101 gcagcagctt tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagca
     5161 gcggcgcacg ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata
     5221 cgaaagcagc aaatctgcgg ttgataaata cccactcgct ggcggaagta tttatcaaca
     5281 gcgacggagt accgactctg ggcgaaggga acgccgactg ccggacgcaa accatcggca
     5341 gccgttcagg attaagttgt aagatggtta actataccct gcaaacaaac ggactcagca
     5401 acacctcaat ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg
     5461 cgtacgatat gcagttcagt ctgaatggca gttcatggaa accggtgagc aatactgcct
     5521 attactacac cttcaacgag atgaagagcg cagactcgat ctatgttttc ttctcgagca
     5581 acttctttaa acagatggtg aacctcggga tcagcgatat caacaccaaa gatctattca
     5641 actttcgctt tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca
     5701 acacgctgat tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc
     5761 agacaccgtc gcgggaggga tatgtcggca gcggcgagtc ggcactcgat ttcggctata
     5821 tcgtaaccac cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg
     5881 cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggctaaag
     5941 taccgttccc ggcgacgctt tcctttatta cccgcaacgg agctacaaaa acctacgatg
     6001 ccgggtgcga tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga
     6061 ctgatatctc tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa
     6121 tggataacgc catttctctg cgtacggtag atgataacgg ctggtttggc gaagtcagcg
     6181 cttcaggaga gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca
     6241 gtggtctgta tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgactttt
     6301 tcactgccgt ccgagactga ctttgtcagc aaacgtgtcg tgaataacaa caaaagcgcg
     6361 cggatatacc gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc
     6421 cgaccggtgg atggtgaact acttttcgcc ccccgccagc tggcgttgca ggctggtgag
     6481 agcgagtatt ttaaatttta ctatcatggt ccacgggata accgcgagcg ctactaccgg
     6541 gtctcatttc gcgaggtccc cactcgtaac ctgacaaaac gcagcccaac cggcggtgag
     6601 gtcagcacgg agccggtggt ggtgatggat accattctgg tagtacgacc acgtcaggtt
     6661 cagtttaaat ggtccttcga tcaggtgacc ggaacggtaa gtaacaccgg caacacgtgg
     6721 tttaagctac tgattaaacc aggatgtgat tcgaccgaag aggaaggcga tgcctggtat
     6781 ctacgtccgg gagacgtggt tcatcagcct gagttacgtc agccggggaa tcattatctg
     6841 gtctataacg acaaattcat taagattagc gattcctgtc cggctaagcc cccttcggcg
     6901 gactaagact tatccatcag acgagggaag aattccacct cagagactca aaaatcgttt
     6961 aaatgatgtg gaagcgatcg tcaatgtcac ctgtaacgat gagctttctc atatgcacac
     7021 cacagtgttc aggagacacg gaaaggtacc aggcggcctg ctaacgagtg gtttcttcac
     7081 taattaggac gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt
     7141 acaccagcca accgcgatac gtccctgcat acgacgtacc gcagttctct ttacgattta
     7201 tagctgtact ggtgaattat gagcaatctg aatccatgca tgacgtgtgg tgcctgttgt
     7261 gcatttttcc gcgtctcttt ttactgggcc gaagccgacg atgctggcgg taccattccc
     7321 gccaggctta ctgaacaaat atcccctttt caccgatgca tgagcggtac caatcagaaa
     7381 aacccccgat gtattgccct tgcaggaaca ccgggcaaaa atgcctgttg cacgatatat
     7441 aaaaatagat cgtccacatg cagagaattc gccatgtctg gtgagaacgg agaagtcaat
     7501 gaggcttgca atcgtgcaag agctaaatac gggctaacac cgttataaat atacaataat
     7561 taattgcatt gccccgccag ctgtaatagc ggggcttcgt ttttcaggga caacaaaacc
     7621 tgaaacttct tccattttcc agcaacaatg cctcttcttc tgtagcgttc tccggatcta
     7681 catcatcctg aagtagcgcg atatgatcac tgactgtccg catcagaata tgaaaagcaa
     7741 cgtttttaac ctataaaaat ggcgctatat ttgcgccatt tttatcattc aaagcattat
     7801 ctatttgagc ctaaagggat ctcaggatct ggctcatggg taattctgtt tcgtaaatct
     7861 ctgcgaataa tttcaataga ccagaaccag actaaatgtc caaaaatttc agagacattc
     7921 tcataccacg gaagatcaaa gagaggtggc gtaagtccca tgagagggaa tgaaatcata
     7981 tgaacaaaaa gttgcgctaa agcacctgcc agtaagccct gccaaagttt aattttcgga
     8041 aatacttcgg cgaccacaca gtaaccaacg gcaaaaacta tcgaaaagat tatgtgcgtt
     8101 acaccaaccc agttaaagac atgcccggcg aaggtataaa cagccgaatt tggatctgtc
     8161 agtcccaacc aatcccgaag aaaaatgtac ggaggattga gaaagttacg ggagcaatca
     8221 atttggcctg cagccctgat taatgattcc ggaccacacg ctgcattaaa catatcaacc
     8281 ggactacgtg gtggcaatgg aacttctgcc ccccacttaa caaatgcgga aacaacgcca
     8341 gcaatcagcc caatgaatgc agcaagacca taacgtctgc ggtttggtgg agtttgttca
     8401 aatatattca tatctaccct gcttgtacca ttatgttata cacctcttta ggagtattca
     8461 taaaacaagg caaatgtaaa gaactgtatt gttttgtata accacatggt tacctaatcg
     8521 ccaattaata taagctccat tatttctcca tatttttatg ttagaagtga caaggagaaa
     8581 tgtaatgttt ctctcagaga atataaatca ttccttttgc ccaccttaat ggtgggcttt
     8641 tttatgcaaa acctgaacgt cctgtggtat tcctctggca taatgatttt cgcgattaac
     8701 caacaaccag accagcaaat acgccaccac cagcacagca acatccatca ggtttgccac
     8761 accccatgcg tgcggctcaa agccgccatg gcatattaat ccgcttgcca ttttcgaaca
     8821 gctgataggt ttagtccggt gtttccctgc ttgcacttaa aaatgagcgg ataggaaact
     8881 ctcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga acacgcacgg
     8941 taaatagatt cgatcctgaa cccatcggtc ccaaatctgt cccaatcaaa aaataaggaa
     9001 ggtattaatg atgtgtaact tattgattta aaatggataa tataaacaga tt
//
LOCUS       DAZFRE010000157         8912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFRE010000157 DAZFRE010000000
VERSION     DAZFRE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.158"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..868
                     /locus_tag="ACHKUI_004670"
     CDS             <1..868
                     /locus_tag="ACHKUI_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO6600713.1"
                     /translation="KYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDE
                     RLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRAT
                     TNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYA
                     MGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRK
                     GNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISP
                     AAFREKYHQMAA"
     gene            886..1335
                     /locus_tag="ACHKUI_004671"
     CDS             886..1335
                     /locus_tag="ACHKUI_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600714.1"
                     /translation="MSAIASTPQTEFSWFRYGQYNETGIYISIEKTIIITITVSGGCF
                     KKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRL
                     RLQDQQEETSVTLFQGKMSTDGNNYSIIKGKVSSVLLLLKMLQINHA"
     gene            1751..3283
                     /locus_tag="ACHKUI_004672"
                     /pseudo
     CDS             1751..3283
                     /locus_tag="ACHKUI_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3317..3826)
                     /locus_tag="ACHKUI_004673"
                     /pseudo
     CDS             complement(3317..3826)
                     /locus_tag="ACHKUI_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            3846..4168
                     /locus_tag="ACHKUI_004674"
                     /pseudo
     CDS             3846..4168
                     /locus_tag="ACHKUI_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4746..4961)
                     /locus_tag="ACHKUI_004675"
     CDS             complement(4746..4961)
                     /locus_tag="ACHKUI_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600715.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(4965..5333)
                     /locus_tag="ACHKUI_004676"
     CDS             complement(4965..5333)
                     /locus_tag="ACHKUI_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600716.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            complement(5624..5866)
                     /locus_tag="ACHKUI_004677"
     CDS             complement(5624..5866)
                     /locus_tag="ACHKUI_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600717.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            6360..6510
                     /locus_tag="ACHKUI_004678"
                     /pseudo
     CDS             6360..6510
                     /locus_tag="ACHKUI_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(6728..8818)
                     /gene="iha"
                     /locus_tag="ACHKUI_004679"
     CDS             complement(6728..8818)
                     /gene="iha"
                     /locus_tag="ACHKUI_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6600718.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT         2342 a         2204 c         2143 g         2223 t
ORIGIN      
        1 aaaatacgcg ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt
       61 atttggtgta tccaggagcg gttattacaa ctgggtacag catgaaccct cagacagaaa
      121 acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac
      181 atatggaacc cggcggctcc agacggagct ggcagagaat ggcatcatcg ttggtcgtga
      241 ccgactggca cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag
      301 agcgactacg aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt
      361 cgctcctaca gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga
      421 gggatggttg tacctcgctg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc
      481 catgggagag cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag
      541 ccagcgccca cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata
      601 cgattaccgg gtcatacagg agcagtttgg tctgaaaaca tcaatgtcgc gtaaaggtaa
      661 ctgttacgac aacgctccga tggaaagctt ctggggaacg ctgaaaaatg agagcctgag
      721 ccactatcgt tttaataacc gggatgaagc catctcagta atacgggaat acattgagat
      781 tttctacaat cgtcagcgtc gtcactctcg tctggggaat atctccccgg cagccttcag
      841 ggaaaaatat catcagatgg ctgcttaaaa aaagaacaaa tggttgtgtc cgctattgcc
      901 agtacacctc aaactgagtt ctcatggttc cgatacggac agtataacga aacgggtatt
      961 tacatctcga tcgagaagac cattatcatt acaatcaccg tttctggtgg gtgctttaaa
     1021 aaagaataca gcattgtctc ccacatgctg gtcactgaca ccataacaga ggccaccctg
     1081 tacgaaaacg ggctgtatac gcgccacatc agactcagtc gcccagtatc tgacagcaaa
     1141 taccccttgc cacccggatc tcagctcatt aaaaatatga ccttacgact gcgcctgcag
     1201 gatcagcagg aagaaacatc cgtgacatta tttcagggaa aaatgagtac cgacggaaat
     1261 aactattcca tcatcaaagg taaagtaagc tcagtacttc tgttattaaa aatgctgcag
     1321 attaatcatg cctgatctct gtcccggtat tttagcccga ggtatattct gaaatatcat
     1381 tcatcatgac cggcgtttat acaaacgcca gtcattcgct aacagcttca ccgttccttc
     1441 ccccatccag gttccgcatg agcgtgttgt aatgttcata agcgtattat caggattgtc
     1501 tggaaaattc ccgatacttt ggactattac tcctgaacaa ctgtaagcgt acagcctgaa
     1561 ccgtctggtc agaatctgac gaattagaca aagtggtgtc caccaaataa gtagtgggaa
     1621 ccaaagtgtc agatatgcag aaaaatgtga ctcccggcag gcgaaaaggc tgccctaatt
     1681 atcctcccga atttaaacag cagctcgttg ggggatgcgg acgatttcct ggactctcag
     1741 gagataaaac atgtttactg aagaagaaaa aatccgcgcc attgagcttt atttcaaata
     1801 tggtaagaag ctggctcccg tcgtacgcga actgggctac ccttcgaaaa gaaatctgcg
     1861 gcgttggatc cgctcatggg aagcaggtgg tggcgcaaaa gaatccattc gtcataagca
     1921 ccgttactcc gatgagcaaa aacaggttgc cgttgaacat tatcttaacc acggctgctg
     1981 cctggcgttt accagcagag ccctgggtta tccctgcact gatgtactcg ctcgctgggt
     2041 taatgaactt tatcccgaca gacgacgtat atttaccagt aaggccaacc cggttgcgcc
     2101 ctttgaacca gaagtcaaac gtcaggctgt catggcactc tgtacacgac aagtatcagc
     2161 cagtgagatc gccaggcgta ttggtgtcag tcgtgcggta ttgtataaat agaaagatga
     2221 aattatcggc aacagtgctt accagactat gcgtaaacat aacgaacctt ccctggaggc
     2281 agaacgcgat gcgttgcggg aggaagtcgc ccgactgaat caggaaatac gccgccggca
     2341 gatggagctg gatattctga aaaaggcgga ggaaatcata aaaaaagacc cgggcatcag
     2401 tatcagtcac ctgaacaaca gagaaaaaac aaagatcgct gatgccctga ggcaaacata
     2461 tcccctgaca gaattacttc atgttctggg ccttacccgt agcagttatt tttatcaccg
     2521 ggctgcactg aaagccggtg ataaatacgc cacgatacgt acgatgctga cagatatatt
     2581 taacagtaat taccagtgtt atggctatcg tcgcctgcat gcgatgctca ggcatgaggg
     2641 ggggcggcta tcagaaaagg ttgtacgcag acttatggtg gaggaacagc ttgtcgtcag
     2701 ccgtaaccgt cgtcgtcgct acagctcata ttgcggagaa atcggaccgg ctccggataa
     2761 ccttatcgcc agagatttta aggcgtagca acctaatcag aaatggctga cagatatcac
     2821 ggagttccaa ctccctgcag gtaaagtctg gctgtcaccg gtggtggact gcttcgatgg
     2881 aaaagttatt agctggtctc tcagtacacg ccccgatgct gagctggtca acactatgct
     2941 ggatagcgct gtcgaaacgt taaatgctgg cgaacgaccg gtgatacaca gtgacagagg
     3001 tgggcattat cgctggccag gctggctgga aagagtgaat gcagcaggtc ttattcgctc
     3061 aatgtcccgt aaaggatgtt cacctgataa tgccgcatgc gaaggttttt tcggcagact
     3121 gaaaacggaa atgtattatg ggcgtaaatg gtcgggcatc acgccagaaa agttcatgca
     3181 gcatgtagat gcttacatca gatggtataa cgagcggcgt ataaaattat cgctgggtgc
     3241 agtcagccct gaaatgtacc gccaacaatg tgggctggca taataaagca gtctaggaaa
     3301 tcgtccgcat ccccaattca ggggcggttt tttctttacg gtttttcgcc ataaagacta
     3361 ttactatcag actgacaaga ctacccgcga aagccgggag ttccgcgcca agattaacag
     3421 ccaccagata ctggggaatt aatgtactga caccacacat cagggtaatt agtccaacgc
     3481 ctcgtatagt cttcatacca ccaccgataa tcgcgacgat gacgaaagga agcagaatgt
     3541 tgaacagtgc cagttgaaga ataattgtcc cgctgagtgt ggttacaggt aaattaactt
     3601 gttcagcaag tacagagaca ggtataccta ctgccccaaa cgctgtaggg actgtattag
     3661 caaccagtga tgcaatcgct gcttttagtg ggttaaagcc cagagcaatc agtataccaa
     3721 tggggattgc taccgctgta ccgtatccgg ctgccgcctc aagaaatcca ccaaaacacc
     3781 atgagataag aagtacctgg atacgtttgt catcactaac actcgctcaa tcatttggtc
     3841 tgctatctca acagattgct ttcaacatta ttgtcagaat ctggagcaga ttgaagatcg
     3901 catacttcaa ctcgatcagg aaatatctcg cctgttacct gagtgatctc tgtgcaatct
     3961 ggtctgccag ttgcaggctc ttaaaggtgt tggtcaacta atcgcaataa ccctggttgc
     4021 tgaactgggt gatttttcgc gattttccaa tcctgaacag ctcatggctt ttctgggact
     4081 ggtgccaggt gaatattcca gcggaaacag cattcgcccc agaggaataa caaaagttgg
     4141 aaacagcgaa ctgagacatc tgctttacgg atatcaaata aattcagatt atttaacagt
     4201 aaaaccccac ttatgtgatg aatctcataa aaaagcaaag tgaagtaata atatccaatt
     4261 tatccctcat tccttaagta agcgtacact ggccgcgtac accatggcat aatgatatgt
     4321 tgttttactg atatttccgg ctggtaaaat ccccgacggc ccggctgaag ctggtgcagg
     4381 aaagcgggtt aattgtcact acgcgggata acctgtctgg ctggatacaa cgtattgcca
     4441 cagatatgca gcaactgcgt cagtccggaa tgtcagtcat ctccggggat gttggggtct
     4501 caggcacact gaccggcccc ccgtgttgtt tccgggtact gtttttgatt tatgcaacaa
     4561 agtattgcag cgtcgaatat gagctatggc cagatttttg ttaatcgtgg ttgttgcaac
     4621 atgtaaggcg aatgaccaca ctattctggc acgctcgtaa tcacgctgaa attccgcttc
     4681 ctgtgctgcc gtgaagagtt ccgaccagga catacggcga agtgtatgtc tgtcagtaat
     4741 atccgtcatg agctctcagc ttcctccttc gtatttccgt cagaaacaca aacgccccgc
     4801 agagaaaatt cgccaacatt ctccttttta atggcgtgca atgcatcgcg acaggcccgt
     4861 tcggtatgaa atgcctggct gtgcaccgcc agtgtgggct gccgggcatc accggaagag
     4921 acattaaagg gagatgaaag ccagaaaatc agtagccaca tgcgtcacac ctcgtccttt
     4981 ttatgcgggt atctggccag aatattgagc gcctgtaatg gagcttcatg catttcacgg
     5041 atacaacggg catcggtgat gttttccctg taatgcgtgg tttccagcat catatgatgc
     5101 atatgcagca tccgggccag ggctgcacgg agttcttcaa tatgttgttc atgggaatgc
     5161 tgacggggtg gtatccggca gagagggata acctccacat catccgggtg cgcgcgaagc
     5221 cacgcgtcag tgactgtaat gtcgtcagtg taaaaccatg tgccggatgt cccgttggcc
     5281 agaaaacgca ttttccaggc ttctggggtg gcggttgtgc tgttatcttt catggtgcca
     5341 ccactcattc aacaggctta atgctgtcca ggcgcagtct gctcagactg tcgtggctgc
     5401 cacagcggcg tcctgtactt ttgctgtagc actcctgccc cctgatacac catgccgttg
     5461 gcgtttcgta gaggctgaca cgtcgggttt tatctttact ggctagaagc ccggtgtgag
     5521 tttttgtctt tttcatcggt ggggtacaaa cgcagaaatt ttgttgtcat cagtttttgc
     5581 ctgcagaggc ctgaaaagac agcgttttca gaccctgttc aggttagtga atattcttac
     5641 ctggcctttc ggcaatatca atcccctgaa gttgcatgga tatcccatgc ccattgagca
     5701 tctggctgtc catggaatgg ataccgcatg tcaccatcag gttgtaacaa agcgtactca
     5761 gcatggcgag cgcctcttct gaggatttac aatgtgtgtt cataagggcc agaaactgct
     5821 caacaacatc cgggttgatg ctggtactgt gtatttcatc cttcattatg tgtccttgtt
     5881 ttttattatt ttgcatttca gaaaataaca cgatgcatgt aacccagaaa accgatatgt
     5941 gcacgcgtag tcactttctg gcagattccg gtctctgaaa caaaatacga ggtggcatga
     6001 agcggattgt ggcggacagg gaaagtgatt tgattccggg gaaggcgctg tgcagcagtc
     6061 ttatccggca gtttaaaggg gtggacggag accaatgagg cactggagca gattcagtgc
     6121 agaggtgaca tgatttatca aacttaattg tgacaaaatg gcgattgaga aggtgcaatt
     6181 gacttatccg gattcggtcg tggcgaggtg gtgaccctgc attctgctaa ccgggttatg
     6241 agccaggagt cagcgataga caaagggact tatctgaccg ggcccccgga aaaatggcgg
     6301 gagagaatga taccttcctg ctgacgagac cggactttga gatgacgagc gatagtgcgg
     6361 cagtgatgta cagcctgatc ggtagctgca aaacttaacg gaatcgagcc ggaaacgtgg
     6421 ctacgccacg tgatcagtgt aatcaacacc tggcctgcca accgcatgaa agagttgttg
     6481 ccctggaacg tcactctctc tgtacactaa tttttacccc acgtccttca cggtgcgctt
     6541 acactctgga caagtatgta aatgataagt taaaaataat ccgtgcattt cctgtgacga
     6601 tattagttgt tggtaacgaa atctcattag cggatcggaa atgcacttaa cctaatacgt
     6661 tgccactgtt ccgccaggag ttacagtggc aacgtattct accgtcagtg atagcgtttt
     6721 gttattatca gaactgatag ttcagcgaca tccagtaatt tcgctcaggt atcacatatc
     6781 ctgttgttga tgatcccgtc tggaagtaat caccggcata cagcgtactc ttaccggcac
     6841 tgtacaggct cacgtcactg taatccttgt tgagcaggtt attcaccgca gcattcagcg
     6901 tcagggcatc cgtcatcttc cacgacagac ctgcatccac caccgtccag gctttcaggt
     6961 attctccttt ctcatcatac actttcttct gtacagcgct cagtgacgaa taattctggg
     7021 tgaaacgtgg tgttttcccg cgataacggg cacccagcca tgatgccacc tcttcggtga
     7081 tctgccagtt cagtttcgca ttcaccatgt gttcaggggt ataactcagc ggcgcacctt
     7141 tgttatcacc atcacgttgt tcacttcggg tccaggtgta attcagtgac agcgtgacat
     7201 cctctgacca cagcggcaat gtgccggcaa attccacacc atgcaaccgg gcctttccgc
     7261 tgtttacata gctattggtg ttatcattta tggaataaga gacaatcttg ttggagaagt
     7321 cagtcataaa acctgtgaca ttggcattca gaccggcggg gttatcgtaa tacaccccag
     7381 cctcataact gacgctctct tccggcttca ggtcggggtt accaagtaga tttgtttttc
     7441 cctgcccgga tacaccacta atccctttat gtagctgccc cattctgggt gccttatatc
     7501 ccgtggtcac accgcctttc agcgtccagg catctgccac atcccagacc agatatgcac
     7561 gcggactgaa gtgtcccccg aattgctcat gatgttcata gcggctgccc acagtcagcg
     7621 caagtgcatc cgtgagatgc cactcatcct cagcaaatac cgaccagctt ttctgccgga
     7681 aagtttcacc tgtgctggca aggacaactc cgtctttcat ggacgagctc tgaaactcgc
     7741 cccccaccgt aaccagatga gattctccca gaggggtaag cagtaatgaa ttcaggataa
     7801 ggttcgattc cttaagctcc cgcggctgac cggcaagccc ccatttgtcg cgcttcagta
     7861 cactgcgtac gagctcacga cctttatttt ctgtctcgtt ccagttcaga tacgatttcc
     7921 atgttccgaa ggtgaaagta tgatcatagc cagctgaaat tttgtttcgc tcatagcgca
     7981 gggtccggtc atatcccccc gtcagactcc ccagttgccc atcccggtta tcataacgct
     8041 gccgggtggt atccatatca aaccagagca catcctgcgc cgacgccttc cagtcaagac
     8101 gtgcaccaag attataattc tgtgactccg tgggataagg aatacgcgtg gctgctgtat
     8161 cgctcagtga tgtgaccgat gagccctgac gctgttgtgt gctaccgcgt acctgcaggc
     8221 tgacagaatc atccacaagg ggaccactgc tccagaaatt aaactggctg ctgttacccc
     8281 atttgttgct ttcctgcaga ttcagccctg cattgacgga agagagccat ttgtctgcat
     8341 tctttctggt aatgatattc accacaccgc ccatcgcatc agagccatac agtgtggaca
     8401 tcggccccct gataacctca atacgctcaa tggcggccag agggggcatg aacccggtat
     8461 tcatggcaga aaaaccgttg ggagtcacgt cactgcttcc gccctgacga acaccatcaa
     8521 tcagtatcag cgtgtaactg gctggcattc ctcggatgct gatttccagc ccaccggttt
     8581 tacccgtacc actttcaaca tccacaccct ctactgatct cagagcctcc gccagatcgt
     8641 ggtactggct ggactgcaat tcctcctggc taatcacaga aacactggcg gctgcgttag
     8701 tcagcttttt ctcatagccg gatgcagaga caatcatcac atcctctgca gcagctatgc
     8761 tgctggctga aaatccgaga cagggaatga ctacggaagc cagagtggtt attcgcatat
     8821 tactaatctc cgatcatgtt aaccggcaag acaatacata aatacaaaca ataatgagaa
     8881 taatttatat ttgcctttta caaaaaataa ag
//
LOCUS       DAZFRE010000158         8832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFRE010000158 DAZFRE010000000
VERSION     DAZFRE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.159"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..137)
                     /locus_tag="ACHKUI_004680"
     CDS             complement(<1..137)
                     /locus_tag="ACHKUI_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6600719.1"
                     /translation="MQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAG
                     D"
     gene            complement(101..844)
                     /locus_tag="ACHKUI_004681"
     CDS             complement(101..844)
                     /locus_tag="ACHKUI_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6600720.1"
                     /translation="MTQTESAILAHARRCVPAESCGLVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            complement(850..1548)
                     /locus_tag="ACHKUI_004682"
     CDS             complement(850..1548)
                     /locus_tag="ACHKUI_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6600721.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1548..1877)
                     /locus_tag="ACHKUI_004683"
     CDS             complement(1548..1877)
                     /locus_tag="ACHKUI_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6600722.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1874..4435)
                     /locus_tag="ACHKUI_004684"
     CDS             complement(1874..4435)
                     /locus_tag="ACHKUI_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6600723.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMAKQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGNWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRGFTR
                     SVLSMLTEIFLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGSQLMKAVYDMARKGAQDELRLQLRDGGLLSGSGR"
     gene            complement(4416..4829)
                     /locus_tag="ACHKUI_004685"
     CDS             complement(4416..4829)
                     /locus_tag="ACHKUI_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6600724.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSVDVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(4856..5287)
                     /locus_tag="ACHKUI_004686"
     CDS             complement(4856..5287)
                     /locus_tag="ACHKUI_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6600725.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(5306..6052)
                     /locus_tag="ACHKUI_004687"
     CDS             complement(5306..6052)
                     /locus_tag="ACHKUI_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6600726.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDGWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTGTVEKGKTTTLTVTVEPENATDKTFRAISADPSKATISVKDMTITVAGV
                     KDGKVSIPVISGNGQFAAVAEITVNNVPGG"
     gene            complement(6060..6455)
                     /locus_tag="ACHKUI_004688"
     CDS             complement(6060..6455)
                     /locus_tag="ACHKUI_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6600727.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(6452..6985)
                     /locus_tag="ACHKUI_004689"
     CDS             complement(6452..6985)
                     /locus_tag="ACHKUI_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6600728.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELASALKQQLRIAIKR"
     gene            complement(7001..7354)
                     /locus_tag="ACHKUI_004690"
     CDS             complement(7001..7354)
                     /locus_tag="ACHKUI_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6600729.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(7347..7730)
                     /gene="gpFI"
                     /locus_tag="ACHKUI_004691"
     CDS             complement(7347..7730)
                     /gene="gpFI"
                     /locus_tag="ACHKUI_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6600730.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVMVPSPLATAMIQHGTAEHA"
     gene            complement(7782..8786)
                     /locus_tag="ACHKUI_004692"
     CDS             complement(7782..8786)
                     /locus_tag="ACHKUI_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6600731.1"
                     /translation="MLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKITGKTPVAA
                     YVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAAEGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT         1846 a         2871 c         2094 g         2021 t
ORIGIN      
        1 tcatcaccgg caatacgtat ctggtaccag ccttcgttca tctgacggcg aaagcccggc
       61 acctgtaacg acagggcacg gatggcttcc gctgccgtgt tcacatacag gctgaggcgg
      121 cggccaaatc gttgcaaatc cccgtgaagg cagatgcgtg ccagtggcgg tgacgccaga
      181 cagaatgcgt tcgtcgttgc catttttcgg aatacctctc ccgtttactc agttgttcag
      241 gcagatggtg aagcagctca ccgttgccgc agtaaatggc ggcatggttc ggtaccgaag
      301 caccaaagca gcacagcaga atatcgcccg cctgtgcaga ggacaggggc acccggtaaa
      361 agccggtgac cgccatattg tccaggtaaa ggttctgacc gttgcgccac cagtcatcct
      421 cgcgatgaaa atccggcatt tcagtccccg ccagatggta tgcatcccgg aacagggtgt
      481 aacagtccgt caccccgtgc tcaaagcgcc gtcccgtcag gtgcggcacg cagcggaatt
      541 tgtgaatgtc accccggcag accagccacc agggcagtgc gctttttatc tgcagccgcc
      601 ggtcagcctc gctcagccag ggcagcccac cgggatgact gtggaccagt gccacaatct
      661 ccccctgcat ctctgcccgc agccagtctt ccggtgcaat acgaaaatac gcctccggct
      721 ctgcagagat attcacacaa gggatatacc gctccccctc cggcgttctc accactaagc
      781 cgcacgactc cgcaggcaca caccgccggg catgcgccag aatcgctgat tctgtctgtg
      841 tcattggatt tactgcgaaa gtttgttaat ggaaaggaaa ccgccaaaat tagccaccat
      901 gccgcgcatc tcacacccgc gcatgcactt gctgcatctg tccttacgga tatccgtggt
      961 ggggttgtcg aactcatccg ccaccgcagg accgttatac ccgcattcat ctccccggta
     1021 atcccacata caggtgttcg ccagcatgat gcgaccggga aacagcgccc cgtccgtctc
     1081 ggtcggtgta gccagcacaa acgaggccgt catggctgtc agctgcgaca tctgctccac
     1141 cacccagcgg tcactcagct cctgctccgg gtccgcctcc ggattgcccg caacgaaatt
     1201 caccgcatcc agaaaacggg catacacccg gcggcggacc accgtggccc ccaccagact
     1261 ctgcaggtct tccgccatcc cggtgaccag accgaacaga ttggacaccg tcagcgacgg
     1321 tctggcactg ctgccccggc cgttcatctc aaagccgctg ccgtcaatcg ggtatgcctc
     1381 atacttacgc ccctgccagg tgaccggctc ccctttttca ttcagctcat tacagaaaaa
     1441 ataccgctca ccaccctgta ccgtcaggtc gatttcccag agtaccaccc gcggtgactg
     1501 ctctgactta accgactcgt tcagactttc ttcgcgaata tcctgcatca gttcaccacc
     1561 tgcttaaact ccgcgctgaa ctcaacgcgc aacatcccga cccgcgcaga ccacccggca
     1621 caggtcacct ttatctgccg gtatgcatag ggtggcttcc acaaaaatgc cttccagcca
     1681 ccgtgctctg ccaggaacgc ttccagatgc cgggcctcct cccgggtcac ggaaagcgtc
     1741 accctgtatg ttttcaggtc agcattcagc cctgccgcca tacgctgtga gtacccgtca
     1801 ccaaaacgca cttcacgcac cgatggctgc gagttcacct ccatatccgg cttcactttc
     1861 cagcgaaagg ttttcatcga ccgctccctg ataacagacc gccatcacgc aactgcagcc
     1921 ggagttcatc ctgtgccccc ttgcgggcca tgtcatacac cgccttcatc agctgcgacc
     1981 ccgcctgtcc gttgataccg tcgttctgaa tcaccacgtg attgttctga ttaaaattaa
     2041 taccttcggc ccgccgcatc tgcgccggac ttccggcacc gccgacataa ccaccttccg
     2101 catacccgcg catcagacga tacaggttgc cgacaccaat ccggctggtt gcctccttcg
     2161 tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc atatttgccg ccggttcccg
     2221 taaatcctcc ggttgcaaaa tggaatttcg ccgcagcggc ctgaatggct gtaccgcctg
     2281 acgcggatgc gccgccacca acagccccgc caatagcgct gccgatactc ccgacaatcc
     2341 ccaccatcgc ctgcttcaga aaaatctctg tcagcatgga cagcacagaa cgggtgaaac
     2401 cacgccagtt ctgttcgctg ccggtcagca tcgctgccat attctgtgca ataccgtcaa
     2461 aggtctgcgt ggctgcactt tttacctgcg acatactgtc cgtggcactc tcttcccact
     2521 cactccagcc ggacctgagg cctgccatcc agttcccgcg aagcaggtct tcagccgccc
     2581 aggtcttttt ctgctctgac atgacgttat tcagcgccag cggattatcg ccatactgtt
     2641 ccttcaggcg ctgttccgtg gcttcccgtt ctgcctgccg gtcagtcagc ccccggcttt
     2701 tcgcatcaat ggcggcccgt tttgcccgtt gctgctgtgc gaatttatcc gcctgctgcg
     2761 ccagcgcgtt caggcgctcc tgatacgtaa ccttgtcgcc aagtgcagcc agctggcgtt
     2821 tgtactccag cgtctcatct ttatgcgcca gcagggattt ctcctgtgca gacagctggc
     2881 gacgttgcgc cgcctcctcc agtaccgcga actgactctc cgccttccac aaatcccggc
     2941 gctgctggct gattttctca tttgctccgg catgcttctc cagcgtccgg agttctgcct
     3001 gaagcgtcag cagggcagca tgagcactgt cttcctgacg atcgcccgca gacaccttca
     3061 cgccggactg tttcggcttt ttcagcgtcg cttcataatc ctttttcgcc gccgccatca
     3121 gcgtgttgta atccgcctgc aggattttcc cgtctttcag tgccttgttc agttcttcct
     3181 gacgggcggt atatttctcc agcggcgtct gcagccgttc gtaagccttc tgcgcctctt
     3241 cggtatattt cagccgtgac gcttcggtat cgctctgctg ctgcgcattt ttgtcctgtt
     3301 gactctgctg ctcagccttc tttcgggcgg cttcaagcgc aagacgggcc ttttcacgat
     3361 catcccagta acgcgcccgc gcttcatcgt taacaaaata atcatccttg cgcagattcc
     3421 agatatcgtc cgctttctta aacgcggcct ctgccttaat cagcatctcc tgcgcggtat
     3481 caggacgacc aatatccagc accgcatccc acatggattt gaatgcccgt gctgtcctgt
     3541 ctgcccaggt ttccagcgtg cccatgttct ctttcaagcg tcgggtctgg tcatcaaacc
     3601 ctttcgttgc ggcctcgttc gccgcctgca atgccccggc ttcatcgccg gaacgctgca
     3661 actgagcaac atacgcaatc tgctccgccg tcacgttatg gaactgcttc gccatcgcaa
     3721 tcagccccga cgtcgggtca gtggtcagtt ttccgaaagc ctctgcaacc ttgtccacct
     3781 ccacaccgga tgcagaagca aaacgcgcga cactctggtt gatggcatca aactgttcac
     3841 caccacgcac accggcattc accagggctg ccagtgactc actcgcctgg ttaaacgtca
     3901 gccctgcggc ctgtccggct ctggagagcg tcagcatgcg atcggcagtc agtccggact
     3961 gattaccgga aagaaccagg gttttattaa acgctgaaag cgtggaatct ccctggtacc
     4021 aggcgtacac cagcgcacct gtcgccaccg ccagcgaggt gaccccgacc atcggcaggg
     4081 tgatcgcacc ggcaagcccc ctgaacatgg ggatcatccc gccgaaggag tccttcacct
     4141 gaccgccctg ttgcagcagg atcagccagg gattctgacc accggcaagc tgcgtggcga
     4201 tatccgtaaa ctgtgcgggc agggttcgca tggccgcttt atactgcccg acggaaatcc
     4261 cggctttttg tgcagccagc gcctggcggc tcaggccctg ttcaacagca ctggcggttt
     4321 ttctggcgtc ggtatccaga cctgaaaaat gacgccttac ccggctcatc tgctcatcga
     4381 aacggacagc atccagactc aggtcaataa caagatcacc aaccggctgg gacatatctc
     4441 acacctcccg gaatccccgc tgaagccatc attaatgcgg catcatccac catgacatcc
     4501 gccacatcca cagacgataa aatatcgcgc cctccgtccc caccgaaccg gacgcctccg
     4561 gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccggcctctc cacctgctct
     4621 tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg atcgcaaaaa
     4681 aacaggctga gtacagcgta cgtcagcccg gaaaaatgca tatccagctg ggtatcctga
     4741 aaataatgcg tgcggtaaaa acggtgccag tcggcatatt cggtggatgt catcccggca
     4801 agcatggcgc gccagtcggg tctccccatc tcacgcgcca gtctgagggc aaagttcagc
     4861 tcgccgtcga agactttccc gcagaaaaat catcatcagt cagcgtgtta tttttcgcca
     4921 cttcagtaat atcagtatcc ggacgaacag cttcgatcat cccggacagg cacaacacca
     4981 cgtcttccgc ccgggcaatg gcatcggcag gccaggtggt gagcacttcc tgctctatct
     5041 tcatcacggc ctcattcatt gacggtgact gcgttttctg tggatggtta tgccacaggg
     5101 acatcgccac cagaaacgcg ccggttctga caagatcttc cacactcacc tgcaggttgc
     5161 cgctggcttc agcctcttct gcccgccgtt tcaggagggc aagatgctca atacgctgca
     5221 gcgcagacag ctcagaaagc gtgacggata caccgttata ttcaaattgt tctgttttca
     5281 ggaacatcgc ttatctctca gctctttagc cacccggcac attattaacg gtaatttcag
     5341 ccaccgcagc aaactgacca ttaccggaaa tcacagggat gctgactttt ccatccttaa
     5401 cccccgccac agtaatcgtc atatctttca cgctaatggt ggcttttgat ggatcggcgg
     5461 aaatcgccct gaatgtctta tccgttgcat tttccggttc cacagtaacg gtcagggtgg
     5521 ttgttttccc tttttcaacc gtacctgtcg gcgtcacctt aatcgcagtg accggcgtaa
     5581 ttttgctgcg ttcttccgct acagaaggtt tacccacgtt agtgactttc accgtgcggg
     5641 tgatcacttc tttcgccgtc acggccttac cgatactgct gacccagcca cgaaacacat
     5701 ccaccgtgcc attcggaaaa cggattttat aggcccggac atcgccgctt tcaaaccagc
     5761 ctataagccc tttctggcct tcctctcccg gtttccaggc cagcgtaaaa ctggtatcac
     5821 ctgcagattt ctgtccctgc ccggtcgcgg tccagtccgc gtcttcatca tccaggtagt
     5881 tatcatcgta gggttcagcc gtcatctcgc ccggcgtcag atccttcacc ttagccagtc
     5941 gctgccagcc atcgtctgac aacgggtttg cataagcatc agccttgccg ttgtaaaccc
     6001 acagagtggt accggcacct tttaccggct caaggggatt tggtgttgcc atatcgtcct
     6061 cacatctcgt atgtaatgga ataagtcaga tctgcagaac tccataacgc catatcgtca
     6121 tcacgacgat actcatagcc ctgcgtaacc atcgtggtaa tcagtcctgc cagtgccggg
     6181 atcgcggtca tcgccgggta aatccggctt tccatccact gatcaagctc tgaatccggt
     6241 acctgtgccg gtaaaaacac ctcaatatgc agcgtggccc gccaggtatc tgcatccagc
     6301 tcttcaccgg tatactctgc atccgtcaga taaaccgcga tcgcaggaaa atcctcttcg
     6361 tcaaaaacaa cggggcgacc atcaaacagc gtcgccccgt gttcatgctg ctcgagtgca
     6421 tccagcactg cggcacgaat gtcagtgtgt ttcatcgttt tatcgcaatc ctcagttgtt
     6481 gtttcagcgc ggatgccagt tctccgggca ggcgttcacg ccggatacgg tcaacattct
     6541 catcaaacgc ctgtttcagt ggggccgcca tcgggatttt caccacatca atagggtaac
     6601 ggtttttccc ggccacacgc tgcatgacat gccagcgacc gttttttaat cgctgaatga
     6661 atgcccgctg ataccgatgc tgaccggctt taagtatgct gttcggacga cggcccagca
     6721 tcctgatccc cagcttaatc actggaagat caccgcggtt aacgataatt tttgcgttcg
     6781 gatttctgac cgtcgcccgt ttcagtctgg accgttcctt taccagtttc cggcgtacct
     6841 ttgtctcccg ggcaacctgt gatgaagact gattaatcgc cgttgtggcc acgcggttaa
     6901 tggccattgc tgaagccgcc ggaatggcgt ttttacgaac ccggctcaga ttatcaatcg
     6961 cctgatcaag cccttttatc gccataattt caccctgcgt ttatcgtcgc cggttaacag
     7021 cgggtggttg cccacggttg agccagagat aacagcttcc cccgtcatcc ggagaaacac
     7081 gatccaccca gaacatctcg ccgttaatgg tcagcgtgtc accacgccgc acggcacgca
     7141 ccgtatccgt ccgcacaaat aatgacgggc tgcttccttc aatacggacc ccgccaccgg
     7201 caaaacccag cgactccgga tcgtcaaaaa ccccctgaac ttcgccgcca cgttgtgctc
     7261 cggaggtgaa ctgcgcacgg atccccatca cttcaacaat cgtgctgtcc accccggcaa
     7321 gggcagcatc aaaggcattc tgaaaatcac gcatgctcag ccgttccgtg ctgtatcatg
     7381 gctgtcgcca gtggtgatgg caccataaca cgcatgcccc ggtacgtcag ttcaacggga
     7441 cggcctgtct ccgggcaata ccccatcaca tgcaggcatt tccgcacacg gaccgcttta
     7501 acatcatccg tagcatcagt gttgtgcaac tgctcaccat cgtctgtgtg attttgatca
     7561 gccccgctct catcagagtg cataatgccc tccggggaaa cagcaagctc ctcttcccac
     7621 tcagacacac gttgagcaat atccgcagca ctccccgaca tatccgcctc gcgccccagc
     7681 aggccagcca gttgacgaag acgattcaga ttttcttctt ttgttgccat ctcagcctcc
     7741 tgtgaaaaaa gacacggggg catttcgccc ccgctcacgg attatttcac ctgtaccacc
     7801 acaaactcat ccggatccgg cagcaccatc agcggtgcgg actgcgtcat ggtgaattca
     7861 cgggccggat cgcccacggt cagccagtgt ttcggataac gggaagaggc caccacacct
     7921 tcggacaacg cctgcgcatc ctgaatggca ccatagcaac gaatgccctc tgctgccgta
     7981 ttccccagga ccagtgtgcc ctccggcaga taacgttttt cggtaccgtc ctctgccaca
     8041 taagacgttt tcgccaccac aatggccaga tcgccgtaat accccttgaa ggacaccacc
     8101 gcccccaggt ctttcactgc cgtttcgagt tgtgaatttg agccgcgacg ggtatccagt
     8161 ttttcgcgga acagcttaaa gccattcagc agacgccaga ccgtaccgtc cataatggcg
     8221 atattcacaa ggccgctggc ctgatcgcag tagaggtcaa tatcatgcgt cggatcaaac
     8281 gtatcacggt cctgctcaga ccatttttta ccgtcagcct gctcaatgtt atttccttca
     8341 gagcgcccga aatccacctc gacagtatca aactgatccc cttccatggt gtatttgcca
     8401 tacagcacgg cattcaccgc ctgcatttct tccacctgga caatggcgtg ctcttcctgt
     8461 ttgaggttat cggtaatgat acgcagacga cggtaggccg ggtcgttcag ctgagccgga
     8521 tcttcaccag gaaggcgctc aaccgcctgc tggtaattaa attcgtgttt gggcttgacg
     8581 tagcccggac gtaacacgcg ggtttcacca ccgcgatggc gaagcacttt tccttcaacg
     8641 atcggggaga cataggccgc caccggcgtt tttccggtaa ttttgtccag catcacctct
     8701 tcggtgtgga aattcaccgt acggcggaaa aacagctcca gaaacagcgc acggaatttc
     8761 actttttgtt cggtataacc gagtattaga cgtcaactag ttttgccgac tcgcgccaac
     8821 tatactggcc gc
//
LOCUS       DAZFRE010000159         8521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFRE010000159 DAZFRE010000000
VERSION     DAZFRE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.160"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..203
                     /locus_tag="ACHKUI_004693"
     CDS             <1..203
                     /locus_tag="ACHKUI_004693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600732.1"
                     /translation="FFPKNSCIICIIHLSCGSLWEVDLSIVIFRKYLSRIGESEKDQT
                     FAKFAKDFAHFANFTVPLCDVL"
     gene            235..816
                     /gene="mdaB"
                     /locus_tag="ACHKUI_004694"
     CDS             235..816
                     /gene="mdaB"
                     /locus_tag="ACHKUI_004694"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6600733.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            847..1161
                     /gene="ygiN"
                     /locus_tag="ACHKUI_004695"
     CDS             847..1161
                     /gene="ygiN"
                     /locus_tag="ACHKUI_004695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6600734.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1206..2093)
                     /locus_tag="ACHKUI_004696"
     CDS             complement(1206..2093)
                     /locus_tag="ACHKUI_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6600735.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2090..3037)
                     /locus_tag="ACHKUI_004697"
     CDS             complement(2090..3037)
                     /locus_tag="ACHKUI_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6600736.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3048..4085)
                     /locus_tag="ACHKUI_004698"
     CDS             complement(3048..4085)
                     /locus_tag="ACHKUI_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6600737.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4094..5077)
                     /locus_tag="ACHKUI_004699"
     CDS             complement(4094..5077)
                     /locus_tag="ACHKUI_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6600738.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5074..5883)
                     /locus_tag="ACHKUI_004700"
     CDS             complement(5074..5883)
                     /locus_tag="ACHKUI_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6600739.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            6257..8398
                     /locus_tag="ACHKUI_004701"
     CDS             6257..8398
                     /locus_tag="ACHKUI_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO6600740.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT         2430 a         2199 c         1945 g         1947 t
ORIGIN      
        1 cattttttcc gaaaaactcc tgtatcattt gcataataca tctctcctgt gggagcttat
       61 gggaagttga tttaagtatt gtcattttca gaaaatattt atcccgcatc ggtgaatcag
      121 agaaagatca gacttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg
      181 taccgctctg tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaaatgagc
      241 aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac
      301 accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt ccgcattgtt
      361 cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg
      421 gtgatctggc agatgcctgg ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt
      481 gatgatgtat tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa
      541 gatccatcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata tatgctttct
      601 ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcagttctt ccacggcgtt
      661 ggtgttgacg gtgtgtacct gccgttccat aaagcaaacc aattccttgg catggaaccg
      721 ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa
      781 gaatatcgca agcatcttgt ggaaattttt ggttaactag agctctggct ttagaaggag
      841 ttaaccatga ttacagtaat cgcagaaatt cgtactcgtc ctggtcaaca tcaccgtcag
      901 gcagtgttgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac
      961 ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat
     1021 tctatcgtga tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc
     1081 ccgcacatga aagcatatag cgaagccgta aaaggtgacg tgctggagat gaatattcgt
     1141 atcctgcagc cggggctttg acatttctaa aaagaggccg gtgctgctac cggtctcagc
     1201 ccagattagc cattctttct tcccgttttc agtcctcgat ggaaatcgtt aattcgctta
     1261 atggttttaa ccgcacgctg cggcactgcg gaagctaccg accagacaat catttccaga
     1321 cacaacaata ccgtaccatg tagcggcgtt ttcccttttt catccccgcg cggaacatga
     1381 atcacgatac tggcgtcttt gctaaaacgt gaatccagcg cgtttgtcag caaaataaca
     1441 gggatcccca gccgcctcgc ctcacgaagc gttgtcagcc cttcccggtg cgcagatttt
     1501 tgcgccatca tgatcagaac atcgccacgt tgaagcgcga tcagttgctc ggcaaggcca
     1561 atacccgtac ggtttagtgc cgtggcaggc aaacctatgc ggttgaatag tcgtgcggta
     1621 tagtccgcca gaatacccga agcgccaatg ccaaaaattg cgacttgccg tgcctgcacc
     1681 aacaacgcta cagcttgcgc aagcgcgtgg cggttttcag gctctgacag gacattacag
     1741 gtatacaaat gcccttcaag aacaaagtcg attgcggtgt taacatcgct cgtcaagtta
     1801 ctcaccgtcg tagacatctt ttcgctggaa ctaagggctg gaccaagcca ctgttccaga
     1861 gtgcgtttca gatcacgcag cccggcaaac cccagggcct gtatagccct gataactgtg
     1921 gcatcggaag tgttcaacgt ggccgcaatt tccatcgccg tttgctcgag taccacttca
     1981 cgatgctcat taatgtagct ggcgacaact cttaacccag gcgttaactg actcgcgcga
     2041 gtgcgaaaac gctcaccaaa aatatcaacc cgcactttat ctttctggat catttagcct
     2101 ccggtagagg tcgccctgca atttgtgatt gtatctgcgc agccatcagc cccagagatg
     2161 caaaggagtt agaaattgcc tcttcacgag aaatcagcac ataacgcccg gaacgacagg
     2221 cagtcagaaa ctgacaccat cccggcatga ctttttccat tgctgccagc tcatcctgag
     2281 gtttaccacc ggtgtcaccg cgccaggttg caaaaacaaa atcggcatcc agttcaggta
     2341 aacgctccgc gctcacatcc attcgcccac cttcaggaat gctctcaatc agcggcggaa
     2401 aacgaaaacc agcatcgcgt aatacccgtc ccagcgaatg atagctatgc atcacattaa
     2461 tttttccctg gtttgcctga atcaccgata ccgttatttt ttggctgtcc agcgtcgcct
     2521 tcagcgcatt gatttgtgcc tgatagcggc gttcaagtat cgccagctgc gactgcgttc
     2581 ctgtaagttc tgccagcttg cgatagattt ctggcgcgcc ccctttgaga tgatcaatgc
     2641 tcaccgtggg cgcaattttt tcaagttgtt caatgggcgt attacgggtc ggttctgtga
     2701 tgatcaagtc aggctttgcc gccacaatcg cttcaatatc aatatcagcg gtaccaataa
     2761 aggcgatcga tgaattatca aagtcgacac ctgtcaataa cgcgccagat cgaatgaaat
     2821 ggctaccgtc aggccgagtt cgcccgtggc tggcgacagg aggtacaccg agctcaatca
     2881 gaggaatagt gatatcaaga tcgtgtaaag agacaattct ctttggatga gcaggaacag
     2941 tgactttgcg ccctaaatca tcagtaaata cctgaacagg ttctgttgca cgggaaaaaa
     3001 atgacaacag tatcagcaga gaaaaaaata aacgcatata actcccgtta aaaactgttc
     3061 ctgcgctgcc agagcagtag taaaaaaaat ggtccaccaa tcagtgagat aatgattccg
     3121 gcaggcagtt gcaaaggcaa aaaagccaaa cgaccaatgc tatccgccag aataactaac
     3181 aacgcccccg ataccgcgct ccccagcagt aatgtcgtct gcccgccacg tagaataaaa
     3241 cgtgatatat ggggtgctat caatccgaca aaaccgatgt taccaacaca cgaaacgcag
     3301 acggctgtca ggattatggg tgcggcgaca cgtaacaacg ccagacgaga actattcacc
     3361 cctaacccgg ttgcaacctg atgaccaagc agagcgatat ccgcagtcct ggcggtgaac
     3421 agcaataaaa gagcggctgg caacatccaa caggctgaaa taccgactaa catccagtta
     3481 gctgcatgca ggcttcccga taaccacatc aatgcagttt gtacatctcg aacatctgca
     3541 gtggtcataa atacaccgat accggcggcg aaaaaccagg aaacaccaat accaatcaga
     3601 acaaatcgcg ggcgggaaat atcgcgcgca caaaaaatca cgattaaagc caccaacagg
     3661 ccaccagcaa gaccagccac tggacgccag aacattccca gcattggaaa ctggaaaatc
     3721 agccacagca cagccacgct acatccttct ttgacgccga ttaaaccagg atcggcaagg
     3781 ccgttacgtg caatggattg cattgcggcc cctgccatac ctaacatggc cccgcatagc
     3841 aatgccatga tgactctcgg caagcgaata tcctgcacaa tataacgtgc gtctgtgctt
     3901 acgttttctg gagcaaacaa cgctcgtcct acttccgatg ccggaacaga gaaagaaccc
     3961 tgcatcagac cgaatatcag tattccaacc gcaagcagga acagcccacc aagcaacagc
     4021 agatttttag ggcgcaccag tgtcgaaaac ttaccaacac gtagtgggcg tagcccgacg
     4081 ttcattatcc gactcatttg aacatcctcg tcgccataat gacaaaaaca ggcgcaccaa
     4141 ctaacgccgt catgacaccg gtcgctaact catggggcgt gaaaagcgtc cgggcaataa
     4201 tatctgccag taataacaaa agcgcgccga cacaagctga aagagggagc agaacgcgca
     4261 gatccgctga taccagacgc cggacaatct gcggtactag caggccgaca aaaccaatgg
     4321 ggccagcaat cgatacggct gcgccacaga gcaacgcgat cgcaagcagg gtgaatgttc
     4381 gcgtgcggag tacagagacg cccagcccct gcgccatgcg atcgcccagt gccaacatgt
     4441 taagggaggg tgcaaggtaa agggctaaca caaatccccc cagagaaaac caggcggacg
     4501 tacccagcgt cgcccaatcc tgccccgcta aatcacctgc tagccatgtc cgcatcgcca
     4561 gcagtgtttg ttcgtcaaga atcaggattg cagccgttat tgaggacacg aacgccgata
     4621 gcgccacgcc gcataaagta actttcattg gcgttaaccc tgaacgccct gcggaggaaa
     4681 gcagcaatat caacaggaac aataacgcgc cccccgtaga tgccagcagt gggcgtccaa
     4741 ccggaaaggc cagtccaagc gcactggcgg caacgacagc aagagcggct ccggcattta
     4801 agcctaaaat atgtggttcc cccaatgggt tacgaatgac agcctgcaac aaagcaccag
     4861 caacaccaag agaagcaccg gtaagcaatg ctgccgctaa acgcggcaaa cggagtctta
     4921 caattacgtt gtgatcgaag ttttgcggat caaaatgaag aaatgcctgc aacaccgttt
     4981 gtggtgcaat aaatcgggca ccaatccctg gatataccac gcatcccacc agcagtaagg
     5041 cgaggaaaat tacagtaacc agccctgtgc gcatcatact ttttctacac cacgaaacgg
     5101 catgaagaac ggctttcccg tcagaggatt tattgatgcg tgaacatcaa cgtcaaagac
     5161 agcttttacc agttcaggcg tgcaatgctc cccctcattc aacacacgca ccactttccc
     5221 ctggcgtaaa aatatcaacg tatcgccata attgacggca aaattcagat catgcaacac
     5281 cacgacgact gtacgcccgt gatgacgcgt taaatcatgt aataactcaa gaatttcaac
     5341 ctgataacgt aaatcaagcc aggttgtggg ttcatccagc aggatataag gcgtcttttg
     5401 cgccaacacc atcgcaatcc agcaacgctg gcgctgccca ccagagagtt tttcaacagg
     5461 taaatgtgca aactcctgcg taccggttaa tttcagggcc tcttccaccg cctgctcatc
     5521 ggcgtcgctc cattgacgta tgaaattttg ccagggaaaa cgcccacgcg acaccagttc
     5581 ataaacggtt aagccctccg gcaataatgg cgactgcggc aagatcccaa gtctacggga
     5641 taacgctttc gttggttgct cgtgaatcgc tttgccatca agcaatatcg aaccgcccat
     5701 aggttgcaag atacgtgcaa tggtgctgag caacgtcgac tttccacagc cgtttgcccc
     5761 aaccagaacg gtcatttttt ctgtgggaac ggagaaagaa acaccatcaa caatgatctt
     5821 cttgtgatac ccggcagaaa cattgtcgag gatcagcccc tgcccttttg cgttatgtgc
     5881 cacgtgtacg ccaacttaat caaagacaaa caaaaataaa actcattctc ctttgagagt
     5941 ttgacgccac aaaatgtagt agtcaagcag ttaaaagcca cacaaaaaca aggtaaataa
     6001 caggtaaaat atttttatac ataaaaataa aaacattaaa tatcaataaa ttaaaagcaa
     6061 tcaaattgta taaacctttc ataaaaacac atgtagcaat tttttgttat ttctcattta
     6121 ctactacatt actgcgtgat tgaatgattc tcaattacat atccaatggc gaaataacaa
     6181 acggatagtg cttagtgagc aataagaaac aattttcact cgcgacaatc cgcgagtttt
     6241 tgggataaca gactaaatgg ctatgttcac accttcattc tcaggactca aaggtcgggc
     6301 gctcttttca ctgctttttg cggcaccgat gattcatgca acagactctg taacgaccaa
     6361 agatggcgaa acaatcactg ttacagcaga tgcaaatacc gcaactgagg caaccgatgg
     6421 ttatcaacct ctgagcacct ctacggcgac attaaccgat atgccgatgc tggatatccc
     6481 gcaggtggtc aatacggtta gcgatcaggt tctggaaaac cagaatgcga caacgctgga
     6541 tgaggcgctt tataacgtca gtaacgtggt acagaccaat acattaggcg ggactcagga
     6601 tgcttttgta cgccgtggat ttggcgcaaa ccgggatggc tccatcatga ccaacggtct
     6661 gcgaaccgta cttcctcgta gtttcaacgc cgcaacagag cgtgtggaag tgctaaaagg
     6721 cccggcctcc acgctgtatg gcattctcga tcctggcgga ctgattaacg tcgtgaccaa
     6781 gcgcccggaa aaaacattcc atggttcggt ttcagccacc tcctccagtt ttggtggcgg
     6841 cactgggcaa cttgatatca caggtcccat tgaaggcact cagctggcgt atcgtcttac
     6901 cggggaagtg caggatgaag attactggcg aaacttcggt aaagagcgca gtacatttat
     6961 tgccccgtca ctcacctggt ttggtgataa tgcaacagta accatgctct attcccatcg
     7021 ggactataaa actccgttcg atcgtggaac gattttcgac cttacgacga aacagcccgt
     7081 aaacgttgat cgaaaaatac gttttgacga accgtttaat attacagatg gtcagtccga
     7141 tctggcgcaa ctcaacgcag aatatcatct caatagccag tggacagcgc gctttgatta
     7201 cagctacagc caagataaat acagcgataa tcaggcgcgt gttaccgcgt atgatgcaac
     7261 gacaggaaca ctgacacggc gtgttgatgc aactcaggga tctacccagc gtatgcatgc
     7321 tactcgtgcg gatctgcaag ggaatgttga tattgccgga ttctataatg agattctggg
     7381 tggggtgtca tatgaatatt atgatcttct gcgtacagat atgattcgct gtaaaaaagc
     7441 taaagatttc aatatataca accctgttta tggtaatacc agcaaatgta caacggtttc
     7501 ggcgtcggac agtgatcaga cgatcaaaca ggagagctac tcagcgtatg cacaggacgc
     7561 actctatctg accgataact ggattgccgt cgccgggatc cgctatcagt attacacgca
     7621 gtatgcgggt aaaggccgtc cttttaatgt caatactgac agccgcgatg aacaatggac
     7681 tcccaaactg gggttggtct acaaactgac gccatcggta tccttatttg ccaattattc
     7741 gcaaacattt atgccacagt cgtcaattgc cagctacatt ggcgatcttc caccagaatc
     7801 atctaatgct tacgaagtag gggcaaaatt cgagctattc gatggtatca ccgcagatat
     7861 tgcgctgttt gatatccata aacgtaatgt gttgtatacc gaaagtgttg gtgatgaaac
     7921 cattgccaaa acggcaggcc gtgttcgttc aagaggggta gaagtcgacc ttgcgggagc
     7981 attaactgaa aacattaata tcattgccag ctacggctat accgatgcta aggttctgga
     8041 agatcctgat tatgcaggta aaccattgcc gaatgttcct cgtcataccg gttcgctatt
     8101 cctgacctat gacattcata acatgcctgg caataacaca ctgacgtttg gcggtggtgg
     8161 acatggcgta agccgtcgtt cggcaaccaa tggggctgac tattatctgc caggctattt
     8221 tgttgccgat gccttcgccg catacaaaat gaaattgcag tatccggtca cactgcaatt
     8281 aaacgtcaaa aacctgtttg ataaaacgta ttacacctct tccatcgcca caaataatct
     8341 gggcaaccag attggcgatc cgcgtgaagt gcaattcacg gtgaaaatgg aattttgatc
     8401 aaaaaagccc ggtatgttta ccgggctgag actgatgaca aactccaaat tgtctgatgc
     8461 gctacgccca tcagatctac catagaaatt caatatatta aattttcata tatttttagg
     8521 c
//
LOCUS       DAZFRE010000160         8194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFRE010000160 DAZFRE010000000
VERSION     DAZFRE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.161"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            233..1216
                     /locus_tag="ACHKUI_004702"
     CDS             233..1216
                     /locus_tag="ACHKUI_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO6600741.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1288..2436
                     /locus_tag="ACHKUI_004703"
     CDS             1288..2436
                     /locus_tag="ACHKUI_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HFO6600742.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2460..4136
                     /locus_tag="ACHKUI_004704"
     CDS             2460..4136
                     /locus_tag="ACHKUI_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO6600743.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4146..4886
                     /gene="kdsB"
                     /locus_tag="ACHKUI_004705"
     CDS             4146..4886
                     /gene="kdsB"
                     /locus_tag="ACHKUI_004705"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6600744.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAAEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4883..6910
                     /locus_tag="ACHKUI_004706"
     CDS             4883..6910
                     /locus_tag="ACHKUI_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO6600745.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFAALRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERTPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTIADLIYQALIVYPTYI
                     HPTLLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6945..8114
                     /locus_tag="ACHKUI_004707"
     CDS             6945..8114
                     /locus_tag="ACHKUI_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO6600746.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSSYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERILYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYY"
BASE COUNT         2115 a         1977 c         2111 g         1991 t
ORIGIN      
        1 aggtgggcaa aatttggttc cctttctcgc ctgtaatatt gcagcgtctt cgcacctcca
       61 tgagacattg cgacttaata agaaggtgat aagtcctgca tataagcatg gactgaccat
      121 ggttttatat tcattaaaaa ttttgttaca acccattgat ttagcataaa taaattatag
      181 tgggttcggg tttgttgtga ctgtggcatt atttccgtgc aaaggagctg atatgtctga
      241 aagacattta cctgatgacc agagcagtac tatcgatcca tatctaatta cctctgttcg
      301 ccagactctg gcagaacaaa gcgcagcatt acaaaacttg tctaaacaac tggattccgg
      361 gcagtaccag cgtgtcctta atttgataat gaactgtaaa gggcacgtta ttctttcggg
      421 aatgggtaaa tcagggcatg tcggtcgtaa aatgtcagcg acgctggcct ctacgggtac
      481 gcctagtttc tttattcatc ctgcagaagc tttccatggc gatctgggca tgattacgcc
      541 ttacgatctt ctgatcctta tttctgccag cggtgaaacg gatgaaatcc tcaagctagt
      601 tccttcactg aaaaatttcg gcaaccgaat tatcgccatt accaataatg gaaattccac
      661 gctggcgaaa aatgctgatg ccgtgctgga actccacatg gcgaatgaaa cctgcccgaa
      721 taatcttgca ccaacaacgt ctaccacgct gacgatggcg atcggcgatg cgctggcgat
      781 tgccatgatc caccaacgca aatttatgcc gaatgatttt gcgcgctatc acccgggcgg
      841 ttcattaggt cgtcgcctgc tgacccgcgt tgctgatgtc atgcagcatg atgttcctgc
      901 ggtacagctg gatgcgtcat ttaaaaccgt gattcaacgt atcaccagcg gatgccaggg
      961 aatggtgatg gtagaagacg cagaaggtgg gctagcgggc attatcaccg acggtgacct
     1021 gcgtcgcttt atggaaaaag aggattctct gacatccgcc acggctgcgc agatgatgac
     1081 acgtgaaccg ctgacgctac cggaagacac catgatcatt gaagcggaag aaaaaatgca
     1141 aaagcaccgc gtctcaacgt tattggtgac caacaaggca aataaagtca ctggccttgt
     1201 gcgcattttc gactaattaa gcaacggggt gcgagaggtt agtctctcat tgagtttgcg
     1261 ccccactgaa actttgataa tcgttacatg ttgataaaag tgaagtctgc cgtatcctgg
     1321 atgcgtgctc gtctgtctgc catctcactg gcagatatcc aaaaacacct ggcgaaaatc
     1381 atcattctgg caccgatggc ggtgctgctg atctatctgg ctatcttcag ccagcctcgc
     1441 tatatgagcg agtcgaaagt cgccattaaa cgctcggatg atttaaacag cggcagcctg
     1501 aattttggtc tgcttctggg tgcctctaac cccagttccg cagaagatgc gttgtatctg
     1561 aaagagtaca tcaactcgcc ggatatgctg gcggcgctgg ataagcaact aaattttcgt
     1621 gaagcgttta gccacagcgg gctcgatttt cttaatcatc ttagcaagga tgaaaccgca
     1681 gaaggcttcc tgaagtacta caaggaccgt atcaacgtct cgtatgacga taaaaccgga
     1741 ttactgaata ttcagacgca gggctttagc ccggagtttg cgcttaagtt taaccaaacc
     1801 gtgctgaaag agtcagagcg ctttatcaat gagatgtcac atcgcatcgc gcgtgaccag
     1861 cttgcctttg cagaaacgga gatggaaaag gcacgccagc gtctggacgc cagcaaagcg
     1921 gaattgctct cttatcagga caacaacaac gttctggatc cacaggcaca ggcacaggcg
     1981 gcgagcacgt tagtgaatac gctgatgggc cagaagatcc agatggaagc ggacctgcgg
     2041 aacttgctga cgtatctgcg tgaggacgcc ccgcaagttg tgagtgcgcg taatgcgatt
     2101 cagtcattgc aggcacaaat tgacgaagaa aaaagcaaaa tcactgcgcc acagggtgac
     2161 aagctaaacc gtatggcagt ggattttgaa gaaatcaaat caaaagtaga gttcaacacc
     2221 gagctgtaca aactgaccct gacctccatt gaaaagaccc gtgtagaagc ggctcgtaag
     2281 ctcaaggtgc tgtcagtgat cagttcgcca cagttgccgc aggaatcgtc ttttccaaat
     2341 atcccttatt tgatcgcctg ctggttactg gtgtgctgcc tgctgttcgg caccctgaaa
     2401 ctgttgctgg ctgttattga agatcaccga gactaacgct gtcgctgaat gagtttgtga
     2461 tgaaattatt taaatcaatt ttactgattg ccgcctgtca cgcggcgcag gccagcgcga
     2521 ccattgatat taacgctgac ccaaacctga caggagccgc gccgcttacc ggtattctga
     2581 acggacagaa gtcggatacg caaaacatga gcggcttcga caataccccg ccgcccgcac
     2641 cgccggtggt aatgagccgt atgtttggtg ctcaactttt caacggcacc agcgcggata
     2701 gcggtgcgac ggtaggattc aaccctgact atattctgaa tccgggtgat agcattcagg
     2761 ttcgcttgtg gggtgcgttc acctttgatg gtgcgttaca ggttgatccc aaaggtaata
     2821 ttttcctgcc gaacgttggt ccggtgaaag ttgctggcgt cagtaatagt cagctaaatg
     2881 ccttggtcac atccaaagtg aaggaagtat accagtccaa cgtcaacgtc tacgcctcct
     2941 tattacaggc gcagccagta aaagtgtacg tgaccggatt tgtgcgtaat cctggtctgt
     3001 atggcggtgt gacgtctgat tcgttactca attatctgat caaggctggc ggcgttgatc
     3061 cagagcgcgg aagttacgtt gatattgtgg tcaagcgcgg taaccgcgtg cgctccaacg
     3121 tcaacctgta cgacttcctg ctgaacggca aactgggact ttcgcagttc gccgatggtg
     3181 acaccatcat cgtcgggcca cgtcagcata ctttcagcgt tcagggcgat gtctttaaca
     3241 gctacgactt tgagttccgc gaaagcagca ttcccgtaac ggaagcgttg agctgggcgc
     3301 gccctaagcc tggcgcgact cacattacga ttatgcgtaa acaggggctg caaaaacgca
     3361 gcgaatacta tccgatcagt tctgcgccag gccgtatgtt gcaaaatggc gataccttaa
     3421 tcgtgagcac tgaccgctat gccggcacca ttcaggtgcg ggttgaaggc gcacactccg
     3481 gtgaacatgc catggtattg ccttatggtt ccactatgcg tgcggttctg gaaaaagtcc
     3541 gcccgaacag catgtcgcag atgaacgcag ttcagcttta tcgcccatca gtggctcagc
     3601 gtcagaaaga gatgctgaat ctctcgctgc aaaagctgga ggaagcctca ctttctgccc
     3661 agtcatccac caaagaagaa gccagcctgc gaatgcagga agcacaactg atcagccgct
     3721 ttgtggcgaa agcacgcacc gtggttccga aaggtgaagt gatcctcaac gaatccaata
     3781 ttgattctgt tctgcttgaa gatggcgacg tcatcaatat tccggagaaa acatcgctgg
     3841 ttatggttca tggcgaggtg ctgttcccga acgcggtgag ctggcagaaa gggatgacca
     3901 ccgaggatta catcgagaaa tgcggtggcc tgacgcaaaa atcgggtaac gccagaatta
     3961 tcgtcattcg tcagaacggt gcggcagtca acgctgaaga tgtagattca ctcaagcctg
     4021 gcgatgagat tatggttctg ccgaaatatg aatcgaaaaa cattgaagtt acccgtggta
     4081 tttccaccat cctctatcag ctggcggtgg gtgcaaaagt gattctgtct ttgtaagggg
     4141 ttgaaatgag caaagcagtt attgtcattc cggctcgcta tggctcctcg cgcctgccgg
     4201 gtaagccact gctcgatatt gttggtaaac cgatgatcca gcatgtttac gagcgtgcgt
     4261 tacaggtggc gggcgttgcg gaagtttggg tggcaacaga cgatccgcgt gttgaacagg
     4321 ccgtacaggc gtttggcggg aaagccatca tgacgcgcaa cgatcatgaa tccggcaccg
     4381 atcggctggt cgaggtgatg cataaagtcg aggcagatat ctacattaac ctgcagggcg
     4441 acgaaccaat gattcgcccg cgggatgtag aaacgctgct acaaggaatg cgtgacgatc
     4501 ccgcgttgcc ggtggcaacg ctatgccacg cgatttctgc cgcagaagcg gctgagccaa
     4561 gcacggtaaa agtggttgtg aatacccgcc aggatgcgct ttatttcagc cgctcaccga
     4621 ttccgtatcc gcgtaatgct gaaaaagcgc gctacctgaa acacgtcggt atttacgctt
     4681 atcgtcgcga tgtgctgcaa aactacagcc agttaccgga gtccatgccg gagcaggcgg
     4741 aatcactgga gcagctaagg ttgatgaacg cggggatcaa catccgcaca tttgaggttg
     4801 ccgcaaccgg tccgggcgtc gacaccccag catgcctgga aaaagtgcgc gccctgatgg
     4861 cacaggaact ggctgaaaac gcatgattgg catttactcg cctggcatct ggcgtattcc
     4921 gcatctggag aaatttctgg cgcaaccgtg ccagaaactt tctctgctgc gccctgttcc
     4981 acaagaagtt gatgctatcg ccgtgtgggg acatcgtccc agcgcggcga aaccagtcgc
     5041 catcgccaaa gcagcgggaa aacccgtcat tcgtctggaa gatggatttg tgcgttcgct
     5101 ggatcttggc gtcaatggcg agccgccgct ttctctggtg gtggatgatt gttgcattta
     5161 ctacgatgcc agcaagcctt cagcactgga gaaactggta caagataaag ccggaaatac
     5221 agctctgata agccaggcca gagaagcgat gcacaccatc gtgaccgggg atttgtcgaa
     5281 atataacctg gcacctgcgt ttgtggctga tgagtcagaa cgttcagaca tcgttctggt
     5341 tgtcgatcag acatttaatg atatgtcagt gacgtatggc aatgctggcc cgcatgagtt
     5401 tgctgccatg ctggaagccg cgatggcgga aaatcctcaa gccgaaattt gggtgaaggt
     5461 gcatccggat gtcctggaag gaaagaaaac aggttatttc gccgctctgc gcgccacgca
     5521 acgagtacgt ttaattgccg agaatgtcag cccgcagtcg ctgttgcgac acgtttcccg
     5581 ggtttacgtc gtgacctccc agtacggctt tgaagccttg ctggcaggaa aaccggtgac
     5641 atgcttcggc cagccctggt atgcaggctg gggcttaacc gacgatcgcc atccgcagtc
     5701 cgctttgtta tctgcccgac gcggttctgc cacgctggag gaactttttg ccgctgcata
     5761 cctgcgttac tgtcgctata tcgatccgca aacgggagaa gtaagcaatc tatttaccgt
     5821 gctgcaatgg ctgcaattac aacgtcgaca tctgcaacag cgtaatggtt atttatgggc
     5881 gccaggctta acgctgtgga agtcagcgat cctgaaacct ttcttgcaaa cggcaacaaa
     5941 ccggctgagt ttttcacgtc gctgtactgc ggcgagcgcc tgcgtggtat ggggtgtaaa
     6001 gggagaacag caatggcgag ccgaagcgca gcgaaaatca ctgccgttat ggcgaatgga
     6061 agatggtttt ctacgttcat ccggacttgg ctctgacctt ttgccgccgc tatcgttggt
     6121 cctggataaa cgcgggatct actatgacgc cacgcgcccc agcgacctgg aagtgctgct
     6181 taatcacagc cagttaacac tggcgcagca gatgcgagct gaaaaattac gccagcgact
     6241 ggttgaaagc aaactgagca aatacaacct gggggccgat ttctctctgc ctgccgaggc
     6301 caaagataaa aaaatcatcc tggtgccggg tcaggtagaa gacgatgcct caattaaaac
     6361 aggcactgtg tcgattaaga gcaaccttga gttattacgc acagtacgcg agcgcactcc
     6421 gcacgcttac attatttata aaccgcaccc ggatgtactg gtggggaatc gcaagggcaa
     6481 tattccgaca gaactaattg ctgaactcgc tgattatcag gcactggacg cagatattat
     6541 tcaatgcatt cagcgcgcag atgaagtgca caccatgaca tcattgtccg ggtttgaagc
     6601 gttattacat ggcaagcaag ttcattgtta cggcctgccc ttctatgccg gttggggttt
     6661 aaccgttgat gaacatcact gcccgcgtcg cgagcaaaaa ttaacgatag cagatttgat
     6721 ctatcaggcg ttgattgttt atccaaccta tatccaccca acactgctac aacctattac
     6781 ggttgaagag gcggcggaat atttgatcca gacgccgcgc aagccgatgt ttattacccg
     6841 aaaaaaagcg ggacgagtaa tacgctatta ccgcaaatta attatgttct gcaaggtcag
     6901 atttggctaa acaatttcac agttgactat tacacaaatt atttatgcaa ggtaatgcac
     6961 taaccgtttt attatccggt aaaaaatatc tgctattgca ggggccaatg ggaccctttt
     7021 tcagtgatgt tgccgagtgg ctagagtcat taggtcgtaa cgctgtgaat gttgtattca
     7081 acggtgggga tcgtttttac tgccgccatc gacaatacct agcttactac cagacaccga
     7141 aagagtttcc cggatggtta cgggatctcc accggcaata tgactttgac acaatcctct
     7201 gctttggcga ctgccgccca ttgcataaag aagcaaaacg ctgggcaaag tcgaaaggga
     7261 tccgcttcct ggcatttgaa gaaggatatt tacgcccgca atttattacc gttgaagaag
     7321 gcggagtgaa cgcatattca tcgctaccgc gcgatccgga tttttatcgt aagttaccag
     7381 atatgcctac gccgcacgtt gagaacttaa aaccttcaac gatgaaacgt ataggccatg
     7441 ctatgtggta ttacctgatg ggctggcatt accgtcatga gtttcctcgc taccgccacc
     7501 acaaatcatt ttccccctgg tatgaagcac gttgctgggt tcgtgcatac tggcgcaagc
     7561 aactttacaa ggtaacacag cgtaaggtat taccgaggtt aatgaacgaa ctggaccagc
     7621 gttattatct tgctgttttg caggtgtata acgatagcca gattcgtaac cacagcagtt
     7681 ataacgatgt gcgtgactat attaatgaag tcatgtactc attttcgcgt aaagcgccga
     7741 aagaaagtta tttggtgatc aaacatcatc cgatggatcg tggtcacaga ctctatcgac
     7801 cattaattaa acggttgagt aaggaatatg gcttaggtga gcgaatcctt tatgtgcacg
     7861 atctcccgat gccggaatta ttacgccatg caaaagcggt ggtgacgatt aacagtacgg
     7921 cggggatctc tgcgctgatt cataacaaac cactcaaagt gatgggcaat gccctgtacg
     7981 acatcaaggg cttgacgtat caagggcatt tgcaccagtt ctggcaggct gattttaaac
     8041 cagatatgaa actgtttaag aagtttcgtg ggtatttatt ggtgaagacg caggttaatg
     8101 cggtttatta ttaacatctt atcaacaatc gacatctcca ttatatcaat cataacagat
     8161 ggatgatacc tttattagat gtaaaaagat aaag
//
LOCUS       DAZFRE010000161         8192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFRE010000161 DAZFRE010000000
VERSION     DAZFRE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.162"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..570
                     /locus_tag="ACHKUI_004708"
     CDS             <1..570
                     /locus_tag="ACHKUI_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600747.1"
                     /translation="NNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGT
                     SFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATR
                     DTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVT
                     FLALPVSTTGKVPAAGRYNALAILRVEYQ"
     gene            744..1454
                     /locus_tag="ACHKUI_004709"
     CDS             744..1454
                     /locus_tag="ACHKUI_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600748.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQV
                     LDEGEKFVGAAGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAKLKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            1457..2224
                     /locus_tag="ACHKUI_004710"
     CDS             1457..2224
                     /locus_tag="ACHKUI_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600749.1"
                     /translation="MQPSPAMQALIEQVYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVVQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYMLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQPEQGTQYQIWEDDWMIDECLCAMYDASSESPGK"
     gene            2227..2832
                     /locus_tag="ACHKUI_004711"
     CDS             2227..2832
                     /locus_tag="ACHKUI_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600750.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRMPPELKNRLHCWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWCSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(2886..4391)
                     /gene="glpD"
                     /locus_tag="ACHKUI_004712"
     CDS             complement(2886..4391)
                     /gene="glpD"
                     /locus_tag="ACHKUI_004712"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6600751.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARREKGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKTPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSQAS"
     gene            4581..4907
                     /gene="glpE"
                     /locus_tag="ACHKUI_004713"
     CDS             4581..4907
                     /gene="glpE"
                     /locus_tag="ACHKUI_004713"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6600752.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAAQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            4952..5782
                     /gene="glpG"
                     /locus_tag="ACHKUI_004714"
     CDS             4952..5782
                     /gene="glpG"
                     /locus_tag="ACHKUI_004714"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6600753.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            5799..6557
                     /locus_tag="ACHKUI_004715"
     CDS             5799..6557
                     /locus_tag="ACHKUI_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6600754.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(6539..8137)
                     /gene="rtcR"
                     /locus_tag="ACHKUI_004716"
     CDS             complement(6539..8137)
                     /gene="rtcR"
                     /locus_tag="ACHKUI_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6600755.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQES
                     RPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKASVNDA
                     DRLRKYLARFGLTWEAMQDQHSSS"
BASE COUNT         1949 a         2006 c         2120 g         2117 t
ORIGIN      
        1 aataatgtac ttttagtttc attgtatgcc agtcagcggc aaacggaaca tgggccagta
       61 ccattattta atgcctggat cagtaacctg gatattcagg ttccgcaagg ctgcacaatt
      121 aatgagggta cgagttttac tgttaatatg ccggatgtgt gggccagtga attgagccgg
      181 gctggtgctg gagcgaagcc tgctggtgtt actcctgtag caacaactat tccgattaat
      241 tgtacgaata aagatacaga tgcggtaatg acgttggtat tcgacggtaa catttccgcc
      301 acacgtgata ccaatgggaa acaaagcatt attcaggcac aggataatcc tgatgttggt
      361 attatgatta tggatagcca gcaaaactcc gtagatttaa atgctctggc aacatcagta
      421 ggcgttccgt tcagattggt ggaaaaccag gctgcttctg cgcaaactgc tgatgttact
      481 ttcctggctt tacctgttag tacaactgga aaagtgcctg cggcaggtcg ttataatgca
      541 ctggctatat tgcgagtgga atatcagtaa tttttgagct agtacagaat cggtttagcc
      601 atcaccgcaa aataaaatgc aataataggg atgaacagaa tgtaaaacaa aaaaatattt
      661 ttaattctac aggggaaaaa cctggtggaa aatgtaggtt atgtgccgat gtataaaacg
      721 ttgcattaag gcgagtaata aaaatggaat tagttaatac attatttgca agcctcgctg
      781 gtactgatcc tttcacaggc gtggatatta cgattgccaa ctgcaaatcg gcatactggg
      841 atgaaggcat tgtccagcaa ctcattaatc aggtcctgga tgaaggggaa aaattcgtcg
      901 gtgctgccgg tcttgaggga ttgcttcgat ataatgtcac attaaatatt ggcttaacct
      961 ccagcaatgt atggccggga ttttccctcg ataccgccac gatcggtcgt ctttgtgcct
     1021 gtggtgctga ttttggattt gacccctata tcagtgatgt accagatgtt caatgtgacc
     1081 tgaatacgac aaatgacttt accgttcatt ttacggcaat gctcaatccg gatgagcggg
     1141 tgatcatagc aaagcgcccg cttaaaaaat gtgactcttg gattgaggat gtttatatct
     1201 ttcaggtgtt taaagaagca tggaaattcc ataccgataa cagcttacgc ggatttcgcg
     1261 ataagcaggc taaactgaaa ctctacgcgc gtcattactc agtggaaaat tgcacagagg
     1321 aaagctgtcg ggattgtaat tattgcattc ggcccagctt ttccctctct cgtagttcca
     1381 ttattcgcct gaatgcggcg aatgctcgtt ttgtttatca accctttaca cgcgaccagc
     1441 ggaagagggg gtaattatgc aacccagccc cgccatgcag gcattaattg agcaggttta
     1501 tcatattttt cgtcgttatc cggtgccgca gaaatttgtg gtttgttgtg aatattgcct
     1561 gagtcagcag gaacaaaagg cattacggaa tacatcgtta cgagcgatcc cgtattcctt
     1621 aatcaatgcc tggaatagta gtccaggacc tgatccgcaa aacagcgatg aggttcgtta
     1681 ttttctgcct cgtttactgg agtttgttgt gcaggggcaa tttgacaata ttcacgaagt
     1741 attttctcta cggcgtataa accttgccag taaagaaaac tggcgtgagg atgaacggga
     1801 aatattgcag caatttgctt gccagtatat gactgattgg gtatccgggg atgaggctgt
     1861 cgaattacag tacatgctgg aaatgttttt ccgtgccgat atcgctcttt cgccattgct
     1921 tgatgccatt atttcagttc ccggattttg gtcggcagcg tctcttgcct gtttattgaa
     1981 tacgtatcgt gacggttata tcagagataa ccaggacgat atcgataaag ccattaccac
     2041 tcaaactaat acttgggcta gtaataatca atccatcctg aaagaacgcg cgcggcaggc
     2101 gattgagaat ccgcttaagc agcccgaaca gggcacacag tatcagatct gggaagacga
     2161 ctggatgatt gatgagtgtt tatgcgcgat gtatgacgca tcatccgaat caccaggaaa
     2221 ataaaaatga ttaaattccg gctttatatt ccccctgtaa ttctcggttt tgttatcgta
     2281 ccattattgg tatggccgac ggttattgcc ttagccgtac ttatattcac gttaactttt
     2341 ctggcggaaa taatattctc ctttccgctc ctggttgtgc gtatttctct tcaggaatta
     2401 caacttgagt taatggttga atatgcactt tttttcagtg taatgggtgg catcggttgg
     2461 caattctccc gcagaatgcc tcctgaatta aaaaacagac tgcattgctg gctggtcttt
     2521 tctccggcct atttctggtt aattctctcg aatttcattc tttatattac tccagagaaa
     2581 tcagcgttgc tggaaaatat ccgaaacttc tttctgacgt ttgtctggct tcccctgaat
     2641 ttttcccctt tttggccgca gccgtggact gattttgtcg gcccgattag tgcacagctt
     2701 ggttttgcgt tgggatatta ctgccagtgg tgtagcaaaa atagaagcca ggggcagaag
     2761 tgggttgagt ggggaacgtg cttaagtttg gcgattttag ctcaggggcc gttattcaat
     2821 tatttacaat gaatagtcta aggaattcaa atagcccggt tgcacttcac aaccgggcat
     2881 catccttacg acgcctgcga caacctctgc tgcgtatatt ccaccagcca ctgactcaca
     2941 cgagattgtt gatccgcatt tagccacatg ccttgtttcg tgcgacgcca cagggcatcg
     3001 tcggcgcggc ggacccattc gtgatccacc agatatttca gctccgcttc gtagaactca
     3061 tgaccgaaat cttcaccgag gtcgcttatc gccccggcat tgccgagcag cagttcgctg
     3121 ttgctgccgt aagtgcgcgc gtagtgacgt gccagcgatt cggtcaggaa cgggtaacgg
     3181 cggcgcaggc gggcggcgta atcgtcacgg tcgccttcaa tggcaccacc cggcagcacg
     3241 ctctctttcg tccacgccgg accaatgccc tgataatatg gcgtcagttt ttccagcgcg
     3301 tgctccgcca gtttgcggta ggtggtcagc ttaccgccga ataccgacag cagcggggtt
     3361 ttgccgtttt catcatggat atcaagggtg taatcacggg taatggcctg cggcgagtcg
     3421 gactcatcat cacatagcgg acgcacgccg gagtaggtcc agacgatatc gtcccggctt
     3481 aactgctttt taaagtgcgt gttatacact ttcaacaggt aattgatttc actctcttca
     3541 atcttcactg cttttggatc gcctttgtat tccacatcgg tggtgccgat gatggaaaac
     3601 tcgtccatcc acgggatcac gaagacaata cgtttatctt cgttttgcag aatgtaggct
     3661 tgcttctggg tatgcacacg cggcaccaca atatggctgc ctttgatcag gcgaatgcca
     3721 taaggtgaag gcagatgcat cccgtcgtcg aaaaattgtt tcacccacgg gccggtagca
     3781 ttaaccaggc cgcgcgcctg ccatgtatat tttttgccgg tatcgatatc ttccgcttcc
     3841 acaatccaca ggcctttttc gcggcgagca gacgtggcac gagtacgagt aagcacttca
     3901 ccgcctttac gcaccaccat ctgggcgttg gcgagtacca gacgggcgtc gtctacccaa
     3961 cagtcagaat attcgaatcc gcgcttaatt tccggtttta acactgagtt tgcgccaaaa
     4021 cgcaaaccgg ttgatcccgg caggctggtg cgcttaccca gatgatcgta cataaacaga
     4081 ccaatgcgaa tcatccacgc cgggcgcaga tgcggacgat gcggcaggcg aaaacgcatc
     4141 gggaaggcga tatgcggggc cattttcagt agcacttcac gttcagccag cgcctcgctg
     4201 accaggcgga attcatagtg ctcaaggtag cgcaggccac cgtgaatgag ttttgaactg
     4261 gcggaagagg tcgcgcaagc gagatcctgc gcctccagca tcagcacgga taaaccgcgt
     4321 ccagcggcgt ctgccgcgat accagcacca ttgatgccgc cacctatcac aatcagatct
     4381 ttggtttcca tgctgccctc attcactttc gttaaagttc ataaatgttc gttatcgaac
     4441 atattagcaa agaatcgcgc tttgggtaac attgaaaaaa cattttagag tgatatgtgt
     4501 aacattatgg cgtttatctg ccgcttcgac gtaaactgtg cggtaaattt gcccacttgt
     4561 ttgtaaagaa agagagacgc atggatcagt tcgaatgtat taacgttgcc gacgcgcacc
     4621 agaagttgca ggaaaaagag gcggtgctgg tcgatattcg cgatccacag agtttcgcaa
     4681 tggggcatgc ggcgcaggct ttccatttaa ccaacgacac attgggcgcg tttatgcgtg
     4741 ataacgactt cgatacgcca gtcatggtga tgtgttacca cggtaatagc agcaaaggcg
     4801 cggcgcagta tctgctgcaa cagggctacg atgtggtcta cagcattgat ggcggctttg
     4861 aagcctggca gcgccagttt cccgcagagg tggcgtacgg cgcgtaacgc tttatactgt
     4921 ccccttttgt gtggaataag cgacagcaac gatgttgatg attacctctt ttgctaaccc
     4981 ccgagtggcg caggcgtttg ttgattacat ggcgacgcag ggtgttatcc tcacgattca
     5041 acaacataac caaagcgatg tctggctggc ggatgagtcc caggccgagc gcgtgcgggc
     5101 ggagctggcg cgttttctcg aaaacccggc agatccgcgt tatctggcgg ccagctggca
     5161 ggcgggccat accggcagtg gcctgcatta tcgccgttat cctttctttg ccgccttgcg
     5221 tgaacgcgca ggtccggtaa cctgggtgat gatgatcgcc tgcgtggtgg tgtttatcgc
     5281 catgcaaatt ctcggcgatc aagaagtgat gttatggctg gcctggccat tcgatccgac
     5341 gctgaaattt gagttctggc gttacttcac ccacgcgtta atgcacttct cgctgatgca
     5401 tatcctcttt aacctgctct ggtggtggta tctcggcggt gcggtggaaa aacgcctcgg
     5461 tagcggtaag ctaattgtca ttacgcttat cagcgccctg ttaagcggct atgtgcagca
     5521 aaaattcagc gggccgtggt ttggcgggct ttctggcgtg gtgtatgcgc tgatgggcta
     5581 cgtctggcta cgtggcgaac gcgatccgca aagtggcatt tacctgcaac gtgggttaat
     5641 tatctttgcg ctgatctgga ttgtcgccgg atggtttgat ttgtttggga tgtcaatggc
     5701 gaacggagca cacatcgccg ggttagccgt gggtttagcg atggcttttg ttgattcgct
     5761 caatgcgcga aaacgaaaat aattccaggg atttagaaat gaaacaaaca caacgtcaca
     5821 acggtattat cgaactggtt aaacagcagg gttatgtcag taccgaggag ctggtggagc
     5881 acttctccgt cagcccgcag actattcgcc gcgacctcaa cgaactggca gagcaaaacc
     5941 tgatcctgcg ccatcatggc ggtgcggcgc tgccttccag ttcggttaac acgccatggc
     6001 acgatcgtaa agccacccag accgaagaaa aagagcgcat cgcccgcaaa gtggcggagc
     6061 aaattcctaa tggctcgacg ctgtttatcg atatcggcac caccccggaa gcggtggcgc
     6121 acgcgctgct caatcacagc aatttgcgca ttgtcaccaa caatcttaac gtcgctaaca
     6181 cgctgatggt aaaagaggat ttccgtatta ttctcgccgg tggcgaatta cgcagccgcg
     6241 atggcgggat cattggcgaa gcgacactcg attttatctc ccagttccgc cttgatttcg
     6301 gcattctggg gatcagcggc attgacagtg acggctcgct gctggagttc gattaccacg
     6361 aagttcgcac caaacgcgcc attattgaga actcgcgcca cgttatgctg gttgtcgatc
     6421 actcgaaatt tggccgtaac gcgatggtca atatgggcag tatcagcatg gtagatgccg
     6481 tctacaccga cgccccgccg ccagtaagcg tgatgcaggt gctgacggac caccatattc
     6541 aactggagct gtgctgatcc tgcattgctt cccatgtcag accaaaacgc gccaggtatt
     6601 tgcgtagccg atccgcgtca ttgacgctgg ctttaccctg gcgcgaaatg tcaaacagtt
     6661 cacgtccggc agcagaaagc gactttgcct ggcggcagat agcgataacg tgttccagtt
     6721 gcatacggtc gaagagatcg atgttttctg cctctgcgcc cagcaacgcc gtaagcgtgg
     6781 aggggcgact ctcctgccag ttatagcgca gacggttgat ctcatcttcc accgtatcca
     6841 gtgtgatgcg tccgctagtg gcaaaggtgg ccatccgcgt gacgctggca gaaagttcgc
     6901 gaaagttacc gcgccatgtt gcctggggag aggtcgcaaa ggccagccag gcacgccgcg
     6961 cttcggtgtt aaaacgcacg ctgtcgccag tgagtgaggc gtggcgctcc acttcataat
     7021 ccaggttcgg ttcaatatct tcctggcgtt ggcgtaatcc tggcaaggtg aaggtccaga
     7081 gatttattcg cgcgtacagg tcttcacgaa atttgccttc ggcaaccaac tggcgcaaat
     7141 cacgcaccgt tccggcgata agctgaaaat cactgctcac ctggcgatcg ctgccaaacg
     7201 ggtaaaaggt tttctcttca atggctttca gcagcattgc ctgttcgtct gcgcccagtt
     7261 cgccaatctc atcaagaaac aacattccgc cgttggcgct gcgtaataat ccttcacgcg
     7321 attcccgcgc cccggtaaac gcgcctttca catgaccaaa cagcgtcgac atggcggtat
     7381 cgccgcgcag ggtggcgcag ttcacttcca caaacgcgcc gctaaactga tgccgcgcct
     7441 gttttagctc gaagatacgt cgtgccagaa atgacttacc tgcaccggtc gggccgttaa
     7501 gcaggatcgg tgcgcgggat tttatcgcca ctttttcaat ctgctcaatc atgcggttga
     7561 agtgggagtt acgcgtggca ataccggact taagaaaatc aagcgtttgc tggcgttcct
     7621 cggcaaagcg gctggcgatg gcgttataac ggcttaaatc gagatcgata atcgtcactg
     7681 cgccagggct atcgggccgc tcttttttgc gcggtggtga agattgtatc aaccgggtgg
     7741 gcaggtaacg cgcttctgcc agcagaaacc agcaaatctg cgcgacgtgg gtgccggtgg
     7801 tgatgtgaat taaatactct tctttatcag gttggaacgc gtaaccacgg gcgaaatcat
     7861 gcaggcaggc gtaaacttct tcgaaatccc acgggttatg cagttcaatc tcaacgccca
     7921 ccacttttgt ttctggcgaa acgctggcga tatcgcgttt aagcgtttcg aacagcgagc
     7981 gcgagcgggc gtcgtgcaat aattccagcc gatcgataac taacgattct tgctggcata
     8041 gacagagtgt cggacgccat tttgaccagc gctgactgcc gcgcccggca taatccagca
     8101 ccgttccgac aaagccaaaa gccactgttt tacgcatctt agatatcctt ataaaagacg
     8161 agatttcatt atcggtaaat cggctttgcg gg
//
LOCUS       DAZFRE010000162         8128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFRE010000162 DAZFRE010000000
VERSION     DAZFRE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.163"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..585)
                     /locus_tag="ACHKUI_004717"
     CDS             complement(<1..585)
                     /locus_tag="ACHKUI_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO6600756.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGE"
     gene            complement(604..888)
                     /locus_tag="ACHKUI_004718"
     CDS             complement(604..888)
                     /locus_tag="ACHKUI_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO6600757.1"
                     /translation="MPLAARLNDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
     gene            complement(1257..1964)
                     /locus_tag="ACHKUI_004719"
                     /pseudo
     CDS             complement(1257..1964)
                     /locus_tag="ACHKUI_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            2082..3118
                     /locus_tag="ACHKUI_004720"
                     /pseudo
     CDS             2082..3118
                     /locus_tag="ACHKUI_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc40 family transposase"
     gene            complement(3194..3673)
                     /locus_tag="ACHKUI_004721"
     CDS             complement(3194..3673)
                     /locus_tag="ACHKUI_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HFO6600758.1"
                     /translation="MIKNIQSRLTPISHEYPSCDECYASLLIYTGNTHPNEISIHLEL
                     EPTSINIKGDEIITLSGKKRYAKNSYWMLSSENLVNSKDIREHLDWLLHKLGPKSSNI
                     AKLQALENISMGIDCTWRSLNWHGGPTLWPEQMKIMANLGLECSFDIYFVGNEDGNL"
     gene            complement(3670..4803)
                     /locus_tag="ACHKUI_004722"
     CDS             complement(3670..4803)
                     /locus_tag="ACHKUI_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFO6600759.1"
                     /translation="MAENHIGCRESKYVAICIAPDVCKVGDAIVPFDSFQDLTHEKIY
                     ISNVRARGNPILTVDCIIAGTRSNAGKGVSSGTSLGSGDCRILTGVDHIKCKGKPVAR
                     NLSLVAMNNDNTVGKLYTQTNPPNQIIPVKESSLWARYLELQRQQQEFELESKKQEAD
                     AMIGLGKAFINGWYMLGALIAKGQMQNDIAQMESNIALARGLGMDTQIMERANETNRE
                     VMETIDPREPLLKPANETQAMAMEVEPWLELMTGVGALVKGLVKFSAKFAIKSVGRMG
                     RSGAKIVLDANGLPIPVPPSVGAAGRVPRVLQTGGNTLEARTARELNEFFGKNINRRE
                     WGKALEKLKKDLRLRGDHHGRILDNGDYIDDSGNILGNIEDYL"
     gene            complement(4796..5737)
                     /locus_tag="ACHKUI_004723"
     CDS             complement(4796..5737)
                     /locus_tag="ACHKUI_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO6600760.1"
                     /translation="MNIENETGFPHFQFEKVGYYGELFTVVVVNQTFDFSYSGGLCLI
                     ADEQRLPLMTDSWFGEPESSSLKTATDLVCRKVRADVLLNGHAWHATGETTRWQASLQ
                     VGNLQRQFDICGPCQWQYISNRWQLSEPELTDRVLLRHELASASEFNPVGIVIPSLIT
                     PGDKSPCPAPQLSASPGAICRSWPSRLRYARGFTEQWQKTVQPFYPDEFDFAFMNYSP
                     PEQQYDGYLIGNEKIILNGLLPSKMEQTCFLPDIRLLAYLFYRSDKNVELRPLLADTL
                     TIHTDDEQLTLLWRLTLPVDSLPQRIVLTTEQRGPHG"
     gene            complement(5753..7699)
                     /gene="tssI"
                     /locus_tag="ACHKUI_004724"
     CDS             complement(5753..7699)
                     /gene="tssI"
                     /locus_tag="ACHKUI_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6600761.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVSFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTILQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRRNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGELPFNPDTSAGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGENLNGQLEQYEIFDYPGRYKDEQHGKDFTLY
                     QMDSLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGSQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRLQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVQ
                     FHWDRYNPATEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPVVMGRT
                     YHEDNRSPGDLPGTKTQMTIRSKTYKGSGFNELRFEDATSNEQVYIHAQKNMDTEVLN
                     DRTTDVKHDHTETIGNDQKITVGLGQTVNVGSKKEGGHDQKVTVANDQTITVQNDQRL
                     DVTHDRHKDGKIIGKDTEEVVKSQDIKVGEDYSLTVTNSLTIKVGECLLKMNKDGTII
                     LNGKSIQIEGKDKINIFGADIDLD"
     gene            complement(7718..8002)
                     /locus_tag="ACHKUI_004725"
     CDS             complement(7718..8002)
                     /locus_tag="ACHKUI_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO6600762.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
BASE COUNT         2076 a         2152 c         1667 g         2233 t
ORIGIN      
        1 ctctcccgcc tgtgacagcc ccgccacatc gtcgcacagc gtcagcttct gctccggact
       61 gtccgccgca aaccgctcaa agaagaaaat cccctcctcc gcccacagcc gcgccagaaa
      121 cgccaggtcc gactccccgt actgcacaca gaactcccgc gccggatggt cctcataaaa
      181 cagcggcgtc cactccgtga cgccgttctc gttcagtaac gtggccgata tcgtccggat
      241 atcctgctgc tggaagatgc ggaagttctg ccgcagcccg caccgccata acggcggctc
      301 aatacgcagg tgataacgca tctgccagcc attgttctcc tgcatgccaa agcccgccac
      361 cacgccggtg acataacgca gcgggatgac accctgccag atagtcagcg ttgcgttctt
      421 ctccagcagc atctccgccg tctgcataaa agaatcgctg gcgacatcca cactcatcac
      481 aaacggataa gactggttct ggattaaccg gaaattcacc actgcaaagg tgtccggctc
      541 ctggccgtca acctccagcg taaagcgaag accttttaag gacataaata cctctcagac
      601 atatcagcct atatttaccg tgccgccacc aataaccacg ccgccgcaat caatagcatc
      661 accggttctg gcggcgggca ggccgtcaat aaagacggtt gacgagcctc tggcgatttt
      721 tcggggatga gggggatgct tcggtttatc atgcggcgtc agcggatctc cctgacgggc
      781 tgcggggaga ccatcgataa agacagtgga tgacccggcg gtgatgacgg tctcgtaata
      841 accgtcgtgc tgggtgcctt tgtcgtttaa tctggcagct aaaggaataa ttcctcctgt
      901 tcagaatttc tacagcagaa ggtttcaaaa atccggagaa atactcaacg aaatattaat
      961 cacttcaccg gatattctga tgcgtacctt cactggtcta tttaagtaat acaaattgtt
     1021 ggcgtgtaat accccattct tctttcatta tctttgcctt taaaacagaa agttcttgaa
     1081 atatatattc aagggaaaaa ctacgataac taccacaagg gtaatataaa ttacaaaatt
     1141 atcgaaaact taaaaaaata atgcgtcaga tctgataaaa aaataagcta tcacgtgtaa
     1201 ctcctgggcg atctcgcaaa aattaaatta taaaaaatac atgacgcagg gcaagattac
     1261 gaaagcccgc tccccgcaag gactgacgcc aggtagtttc tgtccatggc tgcttttcgt
     1321 atcttacgtc ttaaccctgc cctgaatagc gatatgccgt atccctaaaa ataattctgc
     1381 tgagtttcct cttcttattt tgctgtcgtc ttcattcatt accacgtcca gacgccagta
     1441 cagcttattc tccacgtacc agtggtttcg gattgctgtg gcgaacttct ctgcggttaa
     1501 atcagcagaa ctgatataat atctgaccgt catttctggc tctttctttt gttctgttat
     1561 tattgaacga aaggagactg ccatgcataa tttcttcagc cctttccatt caaacgtgaa
     1621 atcaataagt tcatcaggga tatcgcagac aatgtgaaga cgaatttctt ctctgccatg
     1681 acgcttttca ctcattgcat aactgtcatg ctctggatta tttaattctt tcagtggaaa
     1741 tttttcctca aaggttttat taagccgccc ctgatttcat tttcagcgaa taaataatca
     1801 cctccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc atcagttgtg
     1861 atgatttttc ctttaatgtc caacatgtaa agaagttcag ggatagctgt aatctcatta
     1921 gatttctcat ccgtcctgat ctgtccgatg accagactgt gcaataaaat gcgaattatt
     1981 tagtacaaaa agtgtacttc tgatgatctt tccttgccct tgtttattag gtatataccc
     2041 gtgatcattg aagatgcttg atttttcagc aacagaagat catgctgtcc aacatgaaaa
     2101 tattcattac tgagcaacaa aaagctgaac ttgaacgcct tcatgattcc agtcgtgacg
     2161 ggcgagtcag agatcgtatt aaagccattc ttctggcttc cgaaggctgg agttctgcca
     2221 tgattgctca ggctttacgc ctgcatcaga caacgattta tcaccatatc agcgaattcc
     2281 tgaataaagg aaaactgaag cccgaaaacg gcggctcaga cagcaaactc agtgctgaac
     2341 aaacggcctt ttcatcagcc agttatccga taatttattt caccataccc gcgatgttat
     2401 tgcgtttgtc acccggacat ggaacatcat tttcagcatt ccggggatga ataagtggct
     2461 gcatcgtaat ggtttcacct acaaaaaacc gtcaggcgtc ccccataaat tcagtgaaga
     2521 gaaacaaagg caattcattg aatattataa ggaactgaaa acaactgtgg gtgatgagcc
     2581 gattcttttt attgatggag tccatccgac tcaggccact aaaatcagct atggctggat
     2641 acgcaaaggc cagaaaaaag cagtaaaaac aacaggcagc cgcacacgcc tgaatatcat
     2701 gggggcgctg aatctgaaag cactgacgtc accgctaatc tgcgaatata aaaccattaa
     2761 tgaatataat gtatcccgtt ttttcaatga gatacgaaag gtttatcctg attataacca
     2821 gaaaatccac gttattctgg atggagcagg ttaccaccgt tcacagttag tgaaagactg
     2881 ggcggaagtg gtaaatatca ggctgcatta ccttccccct tacagcccaa acctgaatcc
     2941 gatagaaaga atgtggaaat taatgaacga acatgcccgg aataatcgct acttcagcag
     3001 tacgcgagag ttcagggaag caatatctgt cttttttaac cagacactgc cggatatagc
     3061 ggactcactg acatcccgaa ttaacgatca ctttcaggtg ctcacacccg catcttgagg
     3121 tttattgagt atataccata aataccgagt attttattgt gctatttgtt tattaaatat
     3181 ttgcgagcaa taattaaaga ttgccatctt cattgccgac gaaataaata tcaaatgaac
     3241 actccaatcc aagattagcc attattttca tctgctcagg ccataaagtt ggcccaccgt
     3301 gccaattcaa tgatctccag gtacagtcaa tacccattga aatattttct aatgcttgca
     3361 attttgcaat atttgatgat ttaggtccaa gtttgtgaag tagccaatcc agatgttctc
     3421 gaatatcttt cgagttcact agattttctg acgagagcat ccaatatgaa ttttttgcat
     3481 atcttttctt tcccgataga gtaataattt catctccttt tatattgatt gatgttggct
     3541 caagttctaa gtgaatactt atttcattag ggtgagtatt acctgtgtaa atcaagaggc
     3601 tagcatagca ctcatcacaa cttggatatt catgagatat cggggtaagc cttgattgta
     3661 tattcttaat cataaataat cctctatgtt tcctaaaata tttcctgagt catcaatata
     3721 atcaccatta tcaagtattc gaccatggtg atcacctcgt aagcgtaaat cttttttcaa
     3781 cttttctaaa gctttccccc attctctcct gttaatattt ttaccaaaaa attcatttaa
     3841 ctccctggca gttcttgcct caagagtatt acctccagtt tgtaatactc tcggaactct
     3901 tcccgcagcg ccaacgcttg gaggaacagg aataggcaat ccatttgcat ccagcacaat
     3961 tttagcaccg ctcctcccca ttctgccaac cgattttatt gcaaacttcg ctgaaaactt
     4021 gaccaaacct ttcaccagtg ctccaacccc ggtcataagt tccagccatg gctcaacctc
     4081 catcgccatt gcttgggttt cattggcagg tttcaacaat ggctctctag gatcaatagt
     4141 ttccattacc tccctgttgg tctcattcgc ccgctccata atttgggtat ccattcccaa
     4201 ccctctcgcc agagcaatat tactttccat ttgggcaatg tcattttgca tctgaccttt
     4261 ggcaatcaat gcacccaaca tataccagcc attaataaat gccttgccta atcctatcat
     4321 tgcatctgct tcttgctttt tggactcaag ctcaaactct tgctgttgtc tttgcagttc
     4381 cagatacctg gcccagagcg atgattcttt aacaggtatt atctgatttg gcgggtttgt
     4441 ctgggtatac agtttaccaa ctgtattatc attattcatg gcaaccagcg aaagattcct
     4501 ggctaccggc tttcccttgc actttatatg atccacgcct gtcaatatcc ggcagtcacc
     4561 gctgcccaga ctggtgccag aactgacacc tttcccggca ttacttcgtg tacctgcaat
     4621 aatacagtcc acggtcagta ttggattgcc ccgtgccctg acattgctga tataaatttt
     4681 ctcatgtgtc aggtcctgaa agctgtcaaa cggcactatc gcatcaccaa ccttgcagac
     4741 atcaggcgca atacatatcg caacgtactt agattcacga caaccaatat gattttcagc
     4801 catgaggtcc cctctgctcc gtcgtcaaaa caatacgttg tggaagtgaa tccacgggga
     4861 gcgttaagcg ccagagtagt gttagttgct catcgtctgt gtggatggtc agtgtgtcag
     4921 ccaacaatgg gcgcagttct acgtttttat cagagcggta aaacaaatac gccaatagtc
     4981 taatatctgg aaggaaacaa gtttgttcca ttttagaagg taacaggccg ttcaatataa
     5041 ttttttcatt gccaattaaa taaccatcgt attgttgttc tggtggagaa taattcatga
     5101 atgcaaaatc gaattcatct ggataaaaag gctgaacagt cttttgccac tgttctgtaa
     5161 aacctctggc atagcgcaat cgtgaaggcc agctacggca aatagctcct ggtgaggcag
     5221 ataactgtgg agctggacag ggcgatttat cgccaggcgt tatcagggag ggaataacaa
     5281 tgcctacagg attaaattca ctggctgacg ccagttcatg gcgtaacagt acccgatcag
     5341 ttaattcagg ttcgctaagt tgccagcggt tagatatata ttgccattgg caagggccac
     5401 atatatcaaa ttggcgctga agattaccca cctgtaaact ggcctgccat cgagtggttt
     5461 ccccggtggc atgccatgcg tgtccattta ataaaacatc tgccctgact tttctgcaaa
     5521 ccaaatcggt tgctgtttta agactgctgc tttccggttc gccgaaccag gagtcggtca
     5581 tcagtggcag tcgctgctca tccgcaatca gacaaagacc gccgctatat gaaaaatcaa
     5641 atgtctgatt aactaccact actgtgaata actcaccata atatcccact ttttcgaact
     5701 gaaagtgagg aaaaccagtt tcattttcaa tattcatgtt aatttctcca gattaatcca
     5761 gatcaatatc tgcgccgaaa atatttattt tatcttttcc ttcaatctgg atacttttac
     5821 cattgagaat gatagtaccg tccttattca ttttcagtaa gcattcaccg actttaatgg
     5881 tgaggctgtt ggtaactgtc aggctgtaat cttctccaac tttaatatcc tgggatttca
     5941 cgacttcttc ggtatctttt cctattatct taccatcctt gtgacgatcg tgggtaacat
     6001 ccagccgctg atcgttttgt accgtaatgg tctggtcatt cgccactgtc actttctggt
     6061 catgaccacc ttcctttttg ctccccacat tcaccgtctg ccccaacccc accgttatct
     6121 tctggtcgtt accgatggtt tccgtatggt catgtttcac atccgttgtc cggtcgttga
     6181 gcacctccgt atccatgttc ttctgcgcat ggatatacac ctgctcatta ctggtggcgt
     6241 cttcaaaccg cagctcgtta aatccgcttc ctttataggt ttttgaccgg atggtcatct
     6301 gcgtcttcgt tcccggcagg tcacccgggg agcggttatc ttcatggtac gtccgcccca
     6361 tgaccaccgg ctggtccggg tctccgttca ggaaatcaac aatcacctcc tggcccaccc
     6421 gcggtatcgc cagattgccg aaacccggcc ccgcccacgc ctgtgacacc ctcacccagc
     6481 acgaactcgc ctccgttgcc gggttatacc tgtcccagtg aaactgcact cgcacacgtc
     6541 cgtgttcgtc gcagaagatt tcctctcccg caggaccggt gacaatggcg ctctgtggcc
     6601 cgtccacctt cggtttgctt tgcagccgtg gacgccacgt ccggtccgcc ggtatcacct
     6661 ctaactgatt gcccagcgtg gttcctctcc cctggctgcc gtgcagcgcc tgcggctggc
     6721 ttcctgacag tatgctctgt accacctgcc attcacggtt cagcatcttc tgcggatgtc
     6781 ccgtcagcgt aaaccgcgtt cccggccaca gcttcggtga attactctgc cctgtggcct
     6841 tttccgcatc actgcgcagg ctgtccatct ggtacagcgt gaaatccttg ccgtgctgtt
     6901 catccttgta ccttcccgga taatcaaata tctcatactg ttcaagctgt ccgttcaggt
     6961 tctcaccctg ctgctcatac atccccggcc agtccggcac cttaaacgtg taatcctggc
     7021 tctgcaccga cgacgggcgg acatgcgcct cataacggaa catactgaca cactccgtct
     7081 ccgctcctgc cgatgtgtcc gggttaaacg gaagctctcc cgcctgtgac agccccgcca
     7141 catcgtcgca cagcgtcagc ttctgctccg gactgtccgc cgcaaaccgc tcaaagaaga
     7201 aaataccctc ctccgcccac agccgcgcca gaaacgccag gtcgctttcc ccgtactgga
     7261 cacagaattc ccgcgccgga tggtcctcat aaaacagcgg cgtccactcc gtgacgccgt
     7321 tctcgttcag taatgtggcc gatatcgtcc ggatatcctg ctgctggaag atacggaagt
     7381 tccgccgcag cccgcaccgc cataacggcg gctcaatacg caggtgataa cgcatctgcc
     7441 agccgttgtt ctcctgcata ccaaaccctg ccaccacgcc tgtgacataa cgcagcggga
     7501 tgacaccctg caagatagtc agcgttgcgt tcttctccag cagcatctcc gccgtctgca
     7561 taaaagaatc gctggcgaca tccacactca tcacaaacgg ataagactgg ttctggatta
     7621 accggaagct caccactgca aaggtgtccg gctcctggcc gtcaacctcc agcgtaaagc
     7681 gaagaccttt taaggacata aatacctctc agacatatca gcctatattt accgtgccgc
     7741 caccaataac cacgccgccg cagtcaatag catcaccggt tctggcggcg ggcaggccgt
     7801 caataaagac ggttgacgag cctctggcga tttttcgggg atgaggtgga tgcttcggtt
     7861 tagcatgcgg cgtcagcgga tctccctgac gggctgcggg gagaccatcg ataaaaacgg
     7921 tggatgaccc ggcggtgatg acggtctcgt aataaccgtc gtgctgggtg cctttgtcgg
     7981 taagtttggc tgctaaaggc ataattcctc ctgttcagaa tttctacagc agcaggtttc
     8041 aaaaatccgg agaaatattc aacgaaatat taatcacttc accggatatt ctgatacgta
     8101 ccttcactgg tctatttaag taatacaa
//
LOCUS       DAZFRE010000163         8122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFRE010000163 DAZFRE010000000
VERSION     DAZFRE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.164"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            45..1211
                     /locus_tag="ACHKUI_004726"
     CDS             45..1211
                     /locus_tag="ACHKUI_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO6600763.1"
                     /translation="MIEHYIRGFEEELREIRHQIHGNPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1254..2570
                     /locus_tag="ACHKUI_004727"
     CDS             1254..2570
                     /locus_tag="ACHKUI_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO6600764.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2674..3324
                     /gene="fabR"
                     /locus_tag="ACHKUI_004728"
     CDS             2674..3324
                     /gene="fabR"
                     /locus_tag="ACHKUI_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6600765.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            3324..3683
                     /gene="yijD"
                     /locus_tag="ACHKUI_004729"
     CDS             3324..3683
                     /gene="yijD"
                     /locus_tag="ACHKUI_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6600766.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3723..4823)
                     /gene="trmA"
                     /locus_tag="ACHKUI_004730"
     CDS             complement(3723..4823)
                     /gene="trmA"
                     /locus_tag="ACHKUI_004730"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6600767.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4937..5127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5192..7036
                     /gene="btuB"
                     /locus_tag="ACHKUI_004731"
     CDS             5192..7036
                     /gene="btuB"
                     /locus_tag="ACHKUI_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6600768.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLISSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGTYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6981..7838
                     /gene="murI"
                     /locus_tag="ACHKUI_004732"
     CDS             6981..7838
                     /gene="murI"
                     /locus_tag="ACHKUI_004732"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6600769.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1858 a         1920 c         2207 g         2137 t
ORIGIN      
        1 aatccgttaa ttagcctgtt tataagtgaa taaaggaata gccgatgatc gaacactata
       61 ttcgtgggtt tgaggaagaa cttcgcgaga tccgccatca gatccacgga aacccggagc
      121 tgggattaca agaatttaaa accagtgctt tggttgcgga aaagctgcgc cagtggggct
      181 atgaggttga gcaagggctg gcgacaaccg gcgtggtagc cacgttaaaa gtgggcgacg
      241 gagagaaaag tattggcctg cgggcggata tggatgcgtt gccgatttat gaaaatagtg
      301 gcaagccctg ggccagtaaa catcctggtt taatgcacgc ctgcgggcat gacgggcata
      361 ccaccatact gctgggggct gcgcgttatt ttgccgaaac ccgccgtttt aacggcacgt
      421 tacgccttat cttccagcct gcggaagaga tgattaacgg cggtgagatc atggttaaag
      481 aggggctttt tgatcgtttc ccctgcgatg tcattttcgg gatgcacaac atgccgggtt
      541 tgccggtcgg caagtttttc ttccagcctg gggcgctgat ggcgtcaatg gatcagttcc
      601 atattacggt tcgtggttgt ggcggacacg gtgcgatccc gcacaaagcc atcgatccag
      661 tgctggttgc tgcacatatc accaccgcat tacaaagcat tgtgtcgcgc aatgtcgatc
      721 cgctggaagc ggcggtaatt accgtcggca gcattgttgc gggtgaggcg gctaacgtca
      781 ttcctgacag tgctgagatg aaaatcagtg tccgctctct tagccgcgat acccgacaac
      841 ttcttctgac ccgtattcct gctcttgcac aagcccaggc tgccagcttt ggcgctacgg
      901 ctgaggttac gcatgttaac ggtacaccgg tattagtaaa tgacgaggag atggcgcgct
      961 tcgcctggca ggtggcgtgc aaaacatttg gtgaagatcg ggctgagttt ggcatcaaac
     1021 ccctgatggg cagtgaagat ttctctttca tgctggaagc ccaacccaaa ggcggtttcc
     1081 tgttattcgg taatggcgat gtcggggaag gttcctgcat ggtgcataac cccggttacg
     1141 actttaacga tgcaagcctg gtcccggcaa gcagctactg gggcgcactg gttgaagcct
     1201 ggctgcaata aataaaacaa cacacaacaa cactttttgg caaggaacat ggaatgacgg
     1261 cgatcgtaaa tgaaaccgtg agtgtgaaga aaaagcccag cggcaggcgg gtgatctttg
     1321 cctcggcgtt tggcaatgcg ctcgaatttt ttgattttgg cgtttacaac ttttttgtgg
     1381 tctatatcag tacgcttttc ttcccaccca gtgccgatca taacgtcgcg ctgttactgg
     1441 catttgcaac gtttggtgtg agctttttta tgcgtccgct cggtgggatt atcgttggtg
     1501 cctgggccga tcgcttcggg cgtaaaccgg cgatggtgtt cactattgct ctgatgagcc
     1561 tcggtacgct gatgatcggc atcgcaccga cgtatgaaac ggcgggctat tggggaactg
     1621 caacgctggt gctggcgcgt ctaattcagg gcgttgcggc aggtggtgaa gtcggggctt
     1681 caatgtcgct actggtggag tcagctccgg caaatcgtcg cggcttttac agcagttggt
     1741 cactggcgac ccaagggctg gcaacgacct tcggtggggt tgttgcttta ggtctgagcg
     1801 catggctacc gttcgccaca ggttcagaaa ccgtaatggc agaatggggc tggcgtgtgc
     1861 cgttttttat tggcgtgtta ttagcaccta tcggttgctg gttgcgtctg agcctggaaa
     1921 atgacgtacc tgagccagtg cgcaataaaa aagctgctac cagcgaaagc gcgttttctc
     1981 tgctcctgca acataaggcg actatcgtta atggcgtttt actcgctatt ggtagcacgg
     2041 tcgcaaccta tatctccctc ttttattacg gcacctgggc ggcgaagtat ctggcgatgc
     2101 ctcaacatta ttctcatgca gcgatgttac tggcgggggt aattacgttt gtgggtgcgc
     2161 tgttggtggg gatgctatgt gattcggttg ggcgtaaaaa gctgatttta atctcccgtg
     2221 tgatggtgct tctctgtagc tggccgtcat tctggctgtt ggtgaattac ccaagccctg
     2281 gcatgttgct gacggtggtt ttcgtgatgg tcagctttac cacgcttggc ggtgtgccag
     2341 tgatgttgtt gatctctgaa ctgttgccga aacggattcg ggcattaggt ttcgcgctgg
     2401 tttatagcat tggcgtagca atctttggcg gttttgcgca atatttcgcc acgcaatcca
     2461 tcgtgttgct ggatagtctg acggctccgg cgtggtatct ggggggaggc acgttgctgt
     2521 cgatgctggc gttgttgtac gtaaaagaac cggccaaaga attgcagtaa atatgtttta
     2581 ttgcgttacc gttcattcac aacactggag caatccagta tgttcattct ctggtatagt
     2641 gccagcagta cttttggcaa ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa
     2701 gaaaaaaccc gccgttcgct ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc
     2761 ttcgccagcc tgagtttgcg tgaagtggca cgagaagcag gcattgctcc cacctctttt
     2821 tatcggcatt tccgcgacgt agacgaactg ggtctgacca tggttgatga gagcggttta
     2881 atgctacgcc aactcatgcg tcaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc
     2941 cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc cttccggtta
     3001 ttattgcggg aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt
     3061 cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat gccgcgtgcg
     3121 tttactgaag cgcaagccga agcaatggtg acgattgtct tcagtgcggg ggccgaggcg
     3181 ttggatgtcg gcgtcgagca acgccggcaa ttagaagagc gactggtact gcagctgcga
     3241 atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac cacaattatt
     3301 ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt acgctgctgc
     3361 tagcgttggt tgctggctta tcgatcaatg gtacttttgc agcgctgttt agctccattg
     3421 taccattttc tgtattcccg attatttccc tggtgttgac agtttactgc ctgcatcaac
     3481 gttatcttaa tcgcactatg ccggtaggct tgccgggtct ggcagctgct tgttttattc
     3541 tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc ggctctaact
     3601 tcttcccggc agtactgtcc gtcattatgg tgttctggat tggcgcgaag atgcgtaacc
     3661 gtaagcagga agttgctgag taatcggtat catgccggat gcaagcgcat ccggcatggg
     3721 ttttacttcg cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg gaactgatca
     3781 aatagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt cttgcataac
     3841 gtttccggat tgcaggagat atacagaata cgtggatacg cctgcaccat tttctcggtt
     3901 tcactgtcca gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg ataactcttt
     3961 aagtcgattc cttgcaggcg gttaaactcg cgcacaccat tcatcgcctg agtaaattct
     4021 tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagcggcaat gttgtattgc
     4081 gcggcggcga ctgacggctt ggcgatttcg gtggctaata cccgatcaaa attgcgtgcc
     4141 agcgctaatg aaaagttacc gttgccgcag tatagctcca gtaaatcgcc ttttgagcct
     4201 ttggttacgt ccagcgccca ttccagcatc tgaatattca tcgccgcgtt cggttgggta
     4261 aagctgtttt ctacctgacg gtagatcatc tctttccccg cgatcggcag acgttcgtcg
     4321 atgtaatcct gatccagcgc gattttggtt ttcgttgccc gaccaatcag atgcacattc
     4381 agattctgcg cgcgcagtgc atcgcgcagg gcttccgcct gctgacgcca ctcatcatcc
     4441 agctttttat ggtacagcaa ggaaacaacc gcttgattgc ttagcgtagt gaggtaatca
     4501 atctggaaca acttgtggcg cagaatggga ttattacgca cacccgcaat catcgccgtc
     4561 atcaactggt tgataagttc actggcggcg gggaagctat ccacacggat acgacttttg
     4621 gtttgttgat cgaaaatgat gtggtacagg tcatcgccat cgtgccagat gcggaactcc
     4681 gcacgcatcc gataatgact gaccggcgag cgaaacactt ccggaaccag gtcagaaaac
     4741 ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc atactgttct
     4801 gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc ggcgattgta
     4861 gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct acgtctggac
     4921 agtaacttgt tacaacctgt agcatccact tgccggtcct gtgagttaat agggaatcca
     4981 gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc gttatgcgga
     5041 cactgccatc cggtgggaag tcatcatctc ttagtatctt agatacccct ccaagcccga
     5101 agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga tgaaacctgc
     5161 ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc tgctgacggc
     5221 gtgttccgtc acggcatttt ccgcttgggc acaggatacc agcccggata ctctcgtcgt
     5281 tactgctaac cgttttgaac aaccgcgcag cactgtgctt gcgccaacta ctgttgtgac
     5341 gcgtcaggat atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc
     5401 gggcgtggat atcacccaaa acggcggttc aggtcagctc tcatctattt ttattcgcgg
     5461 tacaaatgcc agtcatgtgt tggtgttaat tgatggcgtc cgcctgaatt tggcgggggt
     5521 gagtggttct gctgatctta gtcagttccc tattgcgctt gtccagcgtg ttgaatatat
     5581 ccgtgggccg cgctccgctg tttatggttc agatgcaata ggcggggtcg tgaatatcat
     5641 cacgacgcgc gatcaccccg gaacggaaat ttcagcaggg tggggaagca atagttatca
     5701 gaactatgat gtctctacac agcaacaact gggggataag acacgggtaa cgctgttggg
     5761 cgattatgcc catactcatg gttatgatgt tgtcgcctat ggtaataccg gaacgcaagc
     5821 gcagccagat aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg agcataattt
     5881 tactgatgtc tggagcggct ttgtgcgcgg ctatggctat gataatcgta ccaattatga
     5941 cgcatattat tctcccggct tgccactggt cgatacccgt aaactctata gccaaagttg
     6001 ggatgccgga ctgcgctata acggcgaact gattaaatca caactgattt ccagctacag
     6061 ccatagcaaa gattacaact acgatccgca ttatggtcgt tatgattcgt ccgcgacgct
     6121 cgatgagatg aaacagtaca ccgtccagtg ggcaaacaac atcatcattg gccacggtaa
     6181 tattggtgcg ggtgttgact ggcagaagca gagcacggca ccgggcacag cttatgttga
     6241 ggatggatat gatcaacgta ataccggcat ctatctgact gggctgcaac aagtcggcga
     6301 ttttactttt gaaggcgcag gacgcagcga cgataattct cagtttggtc gtcatggtac
     6361 ctggcaaacc agcgccggtt gggagttcat cgaaggttat cgctttattg cttcctacgg
     6421 aacatcttat aaggcaccaa atcttgggca actgtatggc acctacggta atccgaatct
     6481 gaatcctgag aaaagcaaac aatgggaagg cgcgtttgaa ggcttaactg ctggggttaa
     6541 ctggcgtatt tccggatatc gtaacgatgt cagtgacttg atcgattatg atgatcacac
     6601 cctgaaatat tacaacgaag ggaaagcgcg gattaagggt gtcgaggcga ccgctaattt
     6661 tgataccgga ccactgacgc atactgtgag ttatgattat gttgatgcgc gcaatgcaat
     6721 tactgacacg ccgttgttac gccgtgctaa acagcaggtg aaataccagc tcgactggca
     6781 gttgtatgac ttcgactggg ggattactta tcagtattta ggcactcgct atgataagga
     6841 ttactcatct tatccttatc aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc
     6901 ggttgcgtat ccggtcacct ctcacctgac agttcgtggt aaaatagcca acctgttcga
     6961 caaagattat gagacagtct atggctacca aactgcagga cgggaataca ccttgtctgg
     7021 cagctacacc ttctgaacca cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt
     7081 tgtcggtcta tgacgagatc cggcatctct taccggatct ccattacatt tatgctttcg
     7141 ataacgtcgc tttcccgtat ggcgaaaaaa gcgaagtgtt tattgttgag cgagtggtgg
     7201 aaattgtcac cgcagtgcaa gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca
     7261 ccgccagtac cgtttcactt cctgcattac gcgaaaagtt cgacttcccg gttgttggtg
     7321 tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa tggcattgtc ggattactgg
     7381 caacccgcgg aactgttaaa cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg
     7441 aatgccagat agaaatgctg ggctcggcag agatggttga gttggctgaa gcgaagctgc
     7501 atggcgaaga tgtttctctg gatgcgctaa aacgtatcct acgcccgtgg ttaagaatga
     7561 aagagccgcc agataccgtt gtactggggt gtacccattt ccctctacta caagaagaac
     7621 tgttacaggt gctgccagag ggaacccggc tggtggattc tggcgcagcg attgctcgcc
     7681 gaacggcctg gctgttagaa catgaagccc cggatgcaaa atctgccgat gcgaatattg
     7741 ccttttgtat ggcaatgacg ccagaagctg aacaattatt gcccgtttta cagcgttacg
     7801 gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt tggttgaatg ttgcgcggtc
     7861 agaaaattat tttaaatttc ctcttgtcag gccggaataa ctccctataa tgcgccacca
     7921 ctgacacgga acaacggcaa acacgccgcc gggtcagcgg ggttctcctg agaatctcaa
     7981 cagagaaaag caaaaaaatg cttgactctg tagcgggaaa gcgtattatg cacacccctc
     8041 gccgctgaga aaaagcgaag cggcactgct ctttaacaat ttatcagaca atctgtgtgg
     8101 gcactcgaag atacggattc tt
//
LOCUS       DAZFRE010000164         8084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFRE010000164 DAZFRE010000000
VERSION     DAZFRE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.165"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            787..1473
                     /locus_tag="ACHKUI_004733"
                     /pseudo
     CDS             787..1473
                     /locus_tag="ACHKUI_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1805..2680
                     /locus_tag="ACHKUI_004734"
     CDS             1805..2680
                     /locus_tag="ACHKUI_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO6600770.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2688..3464
                     /locus_tag="ACHKUI_004735"
     CDS             2688..3464
                     /locus_tag="ACHKUI_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO6600771.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            3633..5894
                     /locus_tag="ACHKUI_004736"
     CDS             3633..5894
                     /locus_tag="ACHKUI_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6600772.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTH
                     FGAGDITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5963..7138
                     /gene="senB"
                     /locus_tag="ACHKUI_004737"
     CDS             5963..7138
                     /gene="senB"
                     /locus_tag="ACHKUI_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO6600773.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7661..7966)
                     /locus_tag="ACHKUI_004738"
                     /pseudo
     CDS             complement(7661..7966)
                     /locus_tag="ACHKUI_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         2269 a         1871 c         1894 g         2050 t
ORIGIN      
        1 gtaattattt gatatatcaa aattgttttc ctctattttt gtgtagatag atgttaatgg
       61 cactgggtgc atttattcgt aatttctttt tccatgctgg gccagcagga gcatttgaac
      121 agaaacttga ttaatcatga aaacacatca cttatcgaag aaaaaaatat aaactaacag
      181 aataactata gatgcaggga aagctaagaa taagcttata gctggggctg tttttttgtt
      241 gaatttccag tttgtgactc tgattattac agctatagtc atccagacta taattaaaat
      301 agaactgcca ttgataaaat catatttcaa aataaattct cctgtatttg tcactcaatc
      361 ggtggtttat gtgactatat tcgcaaaaac tggtcgcaga aaagtcctgt tttcagcttg
      421 gaaacccggt aatcagaata atggataggg gaacaaaaat ctctgtatag aacgctccat
      481 tgagggagag aaaacgtgtc attttgaccc gttcggtatt cagaacacac aaaaccgatc
      541 gcgggtcgct ttcgtgcata gttatgcagc catctaaaaa cgattctggc gcgtttttct
      601 ggtttggcct ggtgttttct tgtctttttg cgttttttgc gccagaaagc ggctgagggc
      661 gttttaaggg gtacgtacaa cgggagttat ggtaaatgga tcggtttttc gggaaggatc
      721 gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc ctgaaatcat
      781 gtccagatcc ggcagaacca gcctcatgcg cagttctggc tggtttttga cgttgatcgc
      841 gagggggcag cgattgactg gagcgaccgg aacgctcccg cgccgaacat caccgtaaaa
      901 aatcctgtga acggtcatgc tcacctgctc tacgcgctca atatcgccgt gagaaccgcg
      961 ccggacagct cggttaaggc gctgaaatac gccgctgcga ttgaacgttc gctgtgtgag
     1021 aaactttgtg ctgatgtgaa ctacagcgga ctgatttgta aaaatccgtt ccacctggaa
     1081 tggcaggtga tggagtggcg agaggaagcc tataccctcg atgaactggc tgattatctc
     1141 gatttgagcg cctcagcgcg ccgtagcatc gataaacatt acgggatggg gcgaaactgc
     1201 cacctgttcg aaatgacgcg caaatgggct tacagggcga ttcgtcaggg ctggcccgaa
     1261 ttctcacagt ggcttgatgc cgtgattcag cgtgtcgaaa tgtacaacgc atcgcttccc
     1321 gttccgcttt caccgcctga atgtcgggct attggcaaga gcattgcgaa atacacgcac
     1381 aggaacttca cgccggaaac tttcgcacag tatgtggctg atacgcacac attaacgtac
     1441 gttttcgttc cattggccct caccccccga taacgaccca ggtagcgcgc cgtattcccc
     1501 ccgccggccg ttttcttcga catgtacatc aggcgcggaa ctcatcgggg cgcaggaatg
     1561 aaatcccatt cagactccgg atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt
     1621 gcaaaaacgg gagtgaccat agatccgttt tccaagcgga cccataatat cggctggtaa
     1681 acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat tcattctttg
     1741 atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg tttcagatgg
     1801 gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta gtctgggagc
     1861 ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt tcctgactga
     1921 cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc ttttggcgga
     1981 tcttgctacc gctcaacatc ttattattgg tgaaaaacac gataatgccg agcatcatca
     2041 gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga gtgtgttgct
     2101 tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt ggctgaaaga
     2161 taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa aagggtggtc
     2221 ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc cgctgctcaa
     2281 tgccaacatt ggccgggagc agatattggc actgtacaaa aaaaacgaat ttcctaaagg
     2341 aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca tatctatgca
     2401 tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc agcagcggga
     2461 tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga ttgcaggtgg
     2521 ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg ccaccggcac
     2581 ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag atcatgctga
     2641 ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg atgaatattc
     2701 tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta ctgcttcatg
     2761 gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct atgtctcctg
     2821 caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct ccactttcgc
     2881 ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa caaactgagg
     2941 acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata actaggaaga
     3001 aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata aaggctcaaa
     3061 ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct ctggtcgaat
     3121 cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat tctgaggcat
     3181 cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct ggcgatgcca
     3241 gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg gaaggcacta
     3301 ttgtcagtag tttcttggaa atctcttcag gaaccttttc tcttgaccgc gaagctattg
     3361 ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg gagggaggat
     3421 tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat aattggccgt
     3481 tcaagttcat ttaactgaaa tttaattttc atttttatgc tgaacatttt atcaaacttt
     3541 tccactgtca aaaaaatcaa aaagacagta gaacataatt aataaacaaa tagttatact
     3601 tatctttatt aatattatac ccaaggaaat ttatgaacgt tataaaactg gctatcggct
     3661 caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa aaaactaatt
     3721 ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg aagacgacca
     3781 gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg gacaaaaacc
     3841 tgcaatcact tgacgcaaca gtgcgtagta tgcctggtac ttatactcaa atagatcctg
     3901 gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt gtaaacacta
     3961 tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca acgacgcatg
     4021 gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta gcagttgatg
     4081 ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt agtgcaaata
     4141 tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc cttcgttcac
     4201 gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt ggtggaaaaa
     4261 gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc ggcagttctg
     4321 tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt tatgataaat
     4381 atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca gacgaattta
     4441 acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt gatataacca
     4501 gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt gactttaacg
     4561 taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg tatactttct
     4621 acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc agccggttca
     4681 ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt acccgctatg
     4741 accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg atcgagaaca
     4801 atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg ctgaaggtta
     4861 cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg atcacagggt
     4921 acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac gatattgacg
     4981 ataaagaggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca ccatggcttc
     5041 agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag atgttcttct
     5101 ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca gaaacctggc
     5161 aggcgggttt taacattgat accagagatt tactggtcga acaggatgcc ctgcgcttta
     5221 aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct tatctggttt
     5281 gttctggagg tcgtaaatgc agtctgcctg aggtgattgg caatggctgg gagggcatta
     5341 gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac gttatcgcaa
     5401 agggcttcga actggagatg gattatgatg caggttttgc ttttggccga ctctctttca
     5461 gccagcagca aacagaccag ccaacctcca tcgccagcac ccactttggc gcaggggata
     5521 taaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc ttcgataacg
     5581 cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg tcgcctgatt
     5641 ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag atcccaacga
     5701 ttatcgatct ctatggtact tacgagtaca accgcaacct gacactgaaa ctttcggtac
     5761 aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg atgccaggtc
     5821 ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata tttggcgggg
     5881 acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct ctcttatgtc
     5941 aattttattg caggaacgaa aaatgaatat tttcacttta tccaaagcac cgctatacct
     6001 gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg aacgcgaact
     6061 ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg tctatgaact
     6121 ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg taaatcgtga
     6181 agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg aaaataaata
     6241 cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc agctctatat
     6301 cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa ccggcaaact
     6361 ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt cgcgttttga
     6421 gcattttcgc catatggttt acagccagga ttccgatacc ctgtttgtga gttatagcaa
     6481 tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc tcgacgggac
     6541 gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt atggtctgac
     6601 gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca acgaaattga
     6661 tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac cacaaatcac
     6721 aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg tggtattcga
     6781 ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac gcgacggtaa
     6841 acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca atccgaaata
     6901 taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg atgccaccaa
     6961 atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg tgttgtcgga
     7021 cgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt gggatcctga
     7081 tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa agtcgtgatc
     7141 ggggggaaaa aacatgggat aaaaactccg ttattccact aaactggccg gactaggaaa
     7201 agcaaacatc tctgtcctgc cggtacgcct gttacgccgc tccatgaggc tgaggaatct
     7261 ccagaaaacg agacaggcac agcattgtac gatcaactga tttgtgccaa taattaaatg
     7321 aaatcacagc tatgcctgtt catggggttc taggccggaa ccccagattt ttccgattgt
     7381 ttgttttcta actgattatt cagtagtttt agagggctga cgctcagtgg aacgaaaact
     7441 cacgttaaga ggttttgact atgataaaag tcaaagtagg ggtgagtttg gatatggaaa
     7501 ttattgtact ataaggagag catgaaaaac cctggggcag tcactgaatg agggaccagc
     7561 agcggtaaaa taaccagcat aatttctgca tggtcgattt ttataccgtg acaggctgtt
     7621 ttttatactt gcaccgattc ccgggcacac ttagcctgtg tcaggccggc atatacctca
     7681 gcagactgat atcccgcgcg tttttcttca gccctgatac attcagcccc gcaatggccc
     7741 ggcggtatac catccggccg ccgcacagca cgcattcgaa cgggtcacga ctcaggaact
     7801 gtttcaccat ttgtgcatag cacactttcg ccactgcttc cggtttatcc atccccagag
     7861 cacggtacac ctgcggcagc ttctctccac acacccggtt ggcaaggaac ccgaagtacc
     7921 tcaccatctt aaaaaactga accgccccgg gaatcctgga gactaaactt cctgagaaag
     7981 aggtaaacag gatgactaaa aatactcgtt tttcccccga agtccgtcaa cgggcagtcc
     8041 gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcg
//
LOCUS       DAZFRE010000165         8081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFRE010000165 DAZFRE010000000
VERSION     DAZFRE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.166"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            363..1187
                     /gene="gadX"
                     /locus_tag="ACHKUI_004739"
     CDS             363..1187
                     /gene="gadX"
                     /locus_tag="ACHKUI_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6600774.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1556..2284
                     /gene="gadW"
                     /locus_tag="ACHKUI_004740"
     CDS             1556..2284
                     /gene="gadW"
                     /locus_tag="ACHKUI_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6600775.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2647..5760)
                     /gene="mdtF"
                     /locus_tag="ACHKUI_004741"
     CDS             complement(2647..5760)
                     /gene="mdtF"
                     /locus_tag="ACHKUI_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6600776.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNQVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     TLFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5785..6894)
                     /gene="mdtE"
                     /locus_tag="ACHKUI_004742"
     CDS             complement(5785..6894)
                     /gene="mdtE"
                     /locus_tag="ACHKUI_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6600777.1"
                     /translation="MLTACDDKSAENAAAMMPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7002..7331
                     /locus_tag="ACHKUI_004743"
     CDS             7002..7331
                     /locus_tag="ACHKUI_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600778.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASM
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7281..7808)
                     /gene="gadE"
                     /locus_tag="ACHKUI_004744"
     CDS             complement(7281..7808)
                     /gene="gadE"
                     /locus_tag="ACHKUI_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6600779.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2090 a         1876 c         1899 g         2216 t
ORIGIN      
        1 tcacgttgta aaaaccgaat gcccaacctt taaaaacagg tgggtattcg gctacttatt
       61 aattgcaata atatattacc tgtctattca tcgtgttgat aagatatatg ttatatgaat
      121 gttatggagc atagcgatac tgcttaatat tacgatgatg aattatttgc ttactattaa
      181 ttcctctgcg ttcttaagtc ccctttttat ataataaatc cagtcatcca gcccgagtat
      241 catcgctcat tatcatcctg ttttcccgct tcgtttaaat ttatttatca atcaatttga
      301 cttaagaggg cggcgtgcta cattaataaa cagtaatatg tttatgtaat attaagtcaa
      361 ctatgcaacc actacatggt aattgtctaa ttgcgtacgc aagacataaa tatattctca
      421 ccatggttaa tggtgaatat cgctatttta atggcggtga tctggttttt gcggatgcaa
      481 gccaaattca agtggataag tgtgttgaga atttcgtatt agtttcaaga gacacgcttt
      541 cattatttct cccgatgctc aaggaggagg cattaaaact tcatgtacat aaaaaagttc
      601 cttcattact cgttcatcac tgtaccagag atattcctgt ttttcaggaa gttgcgcaac
      661 tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagggca ttaatctttg
      721 cgttgttatc tgtttttctt gaggatacgc agtttatacc gttacttctg aacgtgttgc
      781 aaccgaatat gcgaacacga gtttgtacgg tcatcaataa taatatcgcc catgagtgga
      841 cattagcccg aattgctagc gaattgttga tgagtccaag cctgttaaag aaaaaattgc
      901 gcgaagaagg tacgtcatat tcacagttgc ttactgagtg taggatgcag cgtgctttac
      961 aacttattgt tatatatggg gtttcaatta agcgagttgc agtatcctgt ggatatcaca
     1021 gcgtgtcgta tttcatttac gtttttcgaa attattatgg gatgacgccc acagagtatc
     1081 aggagcgatc ggcgcaggaa ttaccgaact gtggctcggc ggcaagtatg gctgcgcaag
     1141 ggatttttta cggcactgac cgttctgcgg aaggaataag attatagagt tttactcaga
     1201 cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct ccagactaat
     1261 aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta agttaaatat
     1321 aacttttact ggaaataaga tcagccattt ttttataaac ataagctata cgctgtgtga
     1381 aaatataatc gtgttgcatt tacttattat caattaactg ttatgcaaaa ctactttgtg
     1441 gataaaattt ggtcctacca aatatggcag tttttgcaca cagaaacagt ctggcgtcat
     1501 ttcattagta tactgacatt gaaataatcg cagtaatgaa atataaggga tagtcatggc
     1561 tcatgtctgc tcggtgatcc tcgttcgtcg ttcattcgat attcatcatg aacagcaaaa
     1621 aatatcgttg cataacgaga gtatcctgct gctggataaa aatttggcag acgattttgc
     1681 gttttgttca ctggatacgc gacggctgga tatcgaagag ctgacagttt gccattactt
     1741 acaaaatatt cgtcagttgc cacgcaattt aggattgcat agcaaagacc gtttgttaat
     1801 taaccagtca ccccccatac agctggtgac ggcgattttt gatagtttca atgacccccg
     1861 ggtcaattcg ccgatactga gcaaaatgct ctacctttcc tgtttatcaa tgttttctca
     1921 taagaaagaa ctgatcccct tacttttcaa tagtatcagt actgtttcag gaaaagttga
     1981 acgccttatt agctttgata tcgctaaacg ttggtatcta cgcgatatcg cagaaagaat
     2041 gtataccagc gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
     2101 aatattactc gcctccagga tgtcgatggc cagacgatta ctcgagttac gtcaaatacc
     2161 tctgcatact attgcggaaa aatgtggcta tagcagtacg tcatacttta taaatacatt
     2221 tagacaatat tatggtgtaa cgccacatca gttttcgcaa cattcgccgg gtaccttttc
     2281 ctgacatatt tcgcatttga atattggtca ggatctcacg tctgcttcat gtgaaactct
     2341 ttcctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat
     2401 aggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctaa caattcatac
     2461 gattacggga atgtgggtca gtattgtgct gttcaaattg atgacgaatg gctggagtgg
     2521 tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag
     2581 tggtgaatgg ttagcaggaa agagtaaggc tgaaccctca cgttcaacct tactctcatt
     2641 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     2701 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccattacgc cggtacctac
     2761 cgcgttttgc gcgccagagc cggcaccatg actgataacc agcggcagca cgccgagaat
     2821 aaaggccaga gaagtcatca ggattgggcg taaacgcata cgcgctgctt cgatgattgc
     2881 ctctaccggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     2941 ggcgttcttg gcggaaagcc cgatggtggt cagcaaacca acctggaagt aaacgtcatt
     3001 acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa
     3061 catcaccgag aacggaattg accagctttc ataaagtgcg gcgagggcga ggaacaccac
     3121 gaccagtgaa atggcatata gcgcgggagc ctggtttgag gataacgctt cctgatacga
     3181 cagcccagtc catgagtagc cgacgcctgc cggaagtttt gcgaccaggt ctgccataaa
     3241 tttcatggcg tcaccggtac ttttcccggc agccgcttca cctaaaatct ccattgacgg
     3301 gatgccgttg tagcgttcca gtcgcggtga accataggtc cattcggtag acgaataggc
     3361 agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     3421 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     3481 gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     3541 cgccacaccc atagcctcgg ctttcgcagc gttgacgttc actttgaaca tcggcgtatc
     3601 ttccaggccg ttcgggcgta ccccgatgac ctgattcggt gattgcgctg ccagtgataa
     3661 cagctcgttt cgcgcctggg tcagcttttc gtgccccaga ttgccgttgt ccagcagttc
     3721 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     3781 gacggcttta ttgatactgc ttagcgcaat cattgcccgc tgaataatcg cggtaaccga
     3841 gttttcctcg ccgacacgtt cagaccacgg cttgaggctg ataaacgcca gaccgttgtt
     3901 ttgcccctga ccactgaagc caaagccacc aacggtaaac accgactgaa cattattttt
     3961 ctcttttgtc agataataat ccgtcacctg ttgtagcact ttcgtggtgt taaccatggt
     4021 tgcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     4081 agaggtcggc gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     4141 catgtagcga ccggtacaac gcaacagcga gcgggtgctg tcggtatagt gctgagttga
     4201 tttttcaaac agcgtgttga agcgtgcgaa cagggtgtta ggtttgtgac cgccctccgg
     4261 cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcta caaatactga
     4321 aagcagcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggtcgcacc
     4381 gctcataaag gccatcggca taaacactgc ggaaagaaca acggcaatcc cgaccagcgc
     4441 acgttggatc tgccccatcg atttatgcgt cgcttccttc ggcggtagct tatcttccgc
     4501 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     4561 cagcaccatc ccgaacatag ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     4621 aaacgtcccg agaataacca ccggtacggc aatcgtcggg atgattgtgg cgcggaaatt
     4681 ctgcaaaaac agatacatga ccaggaagac gaggatgata gcctcaacca gcgttttgaa
     4741 aacttcctga atagaaattt tgataaacgg tgtggtgtcg taaggataaa ccgtcttcag
     4801 gcttgcaggg aaataagccg ataaacggtt cagctcctct ttcaccgctc gcgaggtatc
     4861 cagggcgttt gctccggtag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata
     4921 gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgggcgacat cacgcagcag
     4981 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     5041 cagacgcgtc tgcacaatga tcgaggcgtt cagttgctgg tctgccgcct gtggcatgcc
     5101 acccagttga ccaccggaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga
     5161 aggtacctgg ttatatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc
     5221 ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt
     5281 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaatgc
     5341 cgctaccatc aggatattac tgctcgactt atcgacgcta attccctgct gctgcaccgc
     5401 ttcaggtaat gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc
     5461 aggtgatgtc cccgtctcga aggtcagagt gatggaggca ttacctgccg catcgctggt
     5521 tgaagacatg tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac
     5581 cgagtcttct accgtttgcg catcggcacc cggataggta gcgctgatgg taatggtcgg
     5641 tggcgcaatc tgcggatact gcgcaaccgg taagttcatg atcgccagac cgcctgcaag
     5701 catcataata atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat
     5761 gaaagtcccc ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct
     5821 ggaggaaatt gcacgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac
     5881 ccgatcgcca gcctgcaagc cagaggtgac aacccactgg tcgccgatgg ctttgctggc
     5941 ttcgatttcg cgtagctgca cgacatcgtc tttatccaga atgagtgccg ttgctttacc
     6001 ctgggcgttg tgggtgacgc cttcctgcgg caccagtaat acattctggc ggctaccttc
     6061 atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt tggggaaaat
     6121 cgcccgtaac gtcacggagc ccgtggtttc atccaccgtc gggtcggaga atttcagcgt
     6181 gccggtctgg ccgtaacgtt taccattttc cagattgagc tgtactggcg tactgccctg
     6241 aacctgtttg atttgcccac tggcgacctc ttctttcatg cgcaagaaat cctgcaccga
     6301 ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg
     6361 attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg
     6421 cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg ctttggcgac
     6481 ggtgacattg gcttctgctt cattcaactg ggtgcgtgcg gtgtcgtaat cctgacggct
     6541 aacatagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc
     6601 ggtagagagc gctttcgcca gcgaaccttt ggcggagttc agctctgcct gtaaaggcgc
     6661 aggatcaatc tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg
     6721 tttaatgata ataccgccca cctggggacg tatctcggca acttcataag gaacggttct
     6781 accgggcaat tcgctcaaca cattgaccga accgggggag agtgtgacga caccgacctc
     6841 aggcatcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc
     6901 gcagaataac aacggtatta acagttttct tcttctgttc attttagtcc ctgaaaattc
     6961 ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca
     7021 gcggcatagt ggggttgtca gccagtaaga tgggatgcaa gcacagtgat cgacatggtg
     7081 aggtcaacga caaaaaaaat gttgccgttc tgccaacagt ttctgccagc atgcgggcaa
     7141 cgaagggttc tactggtgga tacacatacc aggggaataa agtcgttttc gtctctcctt
     7201 caataatcac cgggggtatt gaaatcagca tctgcatccc tcgtcatgcc agccatcaat
     7261 ttcagttgct tatgtcctta ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc
     7321 atacgtctta atttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc
     7381 gaaatgttga gcgttgacgt gatattgctt tcatttttgt ttttcagaat cagttgcata
     7441 attttgaact gattatgtga aaaacatagt tgtgaattct tatggggcaa gtgtttacca
     7501 taagtaatct caagaagatt tttgatactt tctttacggc tgacaaaata aataggcgct
     7561 ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat
     7621 ttctccagga atttaatcgc ttcttcatca aggatatggt tgtgataggt gtcaatgatc
     7681 accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt
     7741 tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga
     7801 aaaatcataa cttgctcctt agccgttatc gtttcgaaca tgtcatcctt ttcattattt
     7861 acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tgcccttcgt
     7921 gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac gcactccata
     7981 tattgatcct aagtaagatt cctggttgtt atcagtttgt aaccagtaaa caaaaggtta
     8041 acttttgcgg attactgccc aagaataagt tatcaataac c
//
LOCUS       DAZFRE010000166         7812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFRE010000166 DAZFRE010000000
VERSION     DAZFRE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.167"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..3681
                     /locus_tag="ACHKUI_004745"
     CDS             1..3681
                     /locus_tag="ACHKUI_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6600780.1"
                     /translation="MNNNRKLKVLTILVSAAISNLSGISVVSASVVNNLLPYQIYRDF
                     AENKGDFIPGTESLELYNNSGLLVTTLNKAPMPDFSSVDRLLGVATLVNPQYVVSVKH
                     NGGYSRVRFGTSNYTLVDRNNYSARDFHTPRLNKLVTDVVPVEVTSAGTAQGTYKNVT
                     KFPVFYRIGTGTQIYKNEQGYYTLNGAYSYLTGGIVPTPSISDWSFVTNAGNGALASY
                     GTPGDSGSPLLGWDTTLNKWALVGVLRAYAGTTGSTNWYVVIPTVQITANINADTDKP
                     VTSEAGSGDILWTFNSVTGTGQLVQGDNSWDMHGQKDLDLNYGKNLTFSGGGTIVLEN
                     SVTQGAGALTFNDDYVVKPLDDQTWIGGGVIVNDGHVVDWQVNGVSGDALHKLGTGTL
                     KIDGSGINGGSLNTGDGAVILAQRPDGEGKVQAFSRVSLVSGRSIVILSDENQINPDN
                     IYWGYRGGTLDINGNDLTFHKLNAFDDGAIITSNGRLARLTLSLNEKTATIYHGNFKN
                     DLSVTNNTTNNSGDFIVDGGININDNFEQNGGKLFFQGHPVPHAVTSQSVANTLKKLG
                     DDSVLTQPVSFTQDDWETRQFSLKSLVLKDTAFYLGRNAQLTTDISADNSSVTLGSED
                     VYIDLNDSNEKKVQVIPQQGQSKATKDSDKSTFYGNVSLTNNSTLNINEFFTGGINST
                     DSTINISSPGATISEYSTLTRSALTVTDNAQLTSKAGLLSDDNVVLGSGATLSLLNEQ
                     STMPAFYSAAAWDLQGSGSTLNVGSGTMMSGDITAGSKATINIGVSTNQGSPLGIYYS
                     GDIKAPGANVSIDQTLWKLNSSSQMQNLALSNSQLVMTTDGKTSSNITVLDKLIAENN
                     ILYVKPTRPLNEMSVGDIPLITAKNGTNNTQAFKTVPQQMGFHSMAPDVEVVNTDGAT
                     QWRLKGFDVQRNNAALNESQRFMDIRYRNFLSEVNNLNRRMGDLRDTQGEAGAWARFM
                     TTSGSGEGGFTDRHMHLQIGADKKHHFDGGDLFTGIMMTVTDSKASGESYQGKTRSVG
                     GGLYASTLFNSGLYIDVVGKYVHHRDDYTMQTLGLGKADDTAHSWYLGAETGWRYQWK
                     PDVFIEPQAELVYGTLSGNTFNWQYNGMDVSMERKKAKPLIGRTGVEFGKTLDGRDWQ
                     VTAKAGLSYQFDLRNTGATTYRDFAGESTVYNGKDGRMLANIGIDTRIKDNTRIGLTI
                     EKSAFGRYNVDNAINANIRYTF"
     gene            3838..4446
                     /locus_tag="ACHKUI_004746"
     CDS             3838..4446
                     /locus_tag="ACHKUI_004746"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO6600781.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            4544..5935
                     /gene="selA"
                     /locus_tag="ACHKUI_004747"
     CDS             4544..5935
                     /gene="selA"
                     /locus_tag="ACHKUI_004747"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6600782.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LENEQRFLEMLLK"
     gene            5932..7776
                     /gene="selB"
                     /locus_tag="ACHKUI_004748"
     CDS             5932..7776
                     /gene="selB"
                     /locus_tag="ACHKUI_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6600783.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQIHRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         2000 a         1722 c         2106 g         1984 t
ORIGIN      
        1 atgaataata acagaaaatt aaaagtattg actatccttg tatccgccgc aatatctaat
       61 ttgtctggta tctctgtcgt atcagcatct gttgttaata acttgcttcc atatcagata
      121 tatcgagact ttgccgaaaa taagggcgac tttattcctg gaacggaaag tcttgagttg
      181 tataataact caggattatt agtgactacg ttaaataaag ctcccatgcc tgatttttca
      241 tctgtggata ggctattggg cgttgccaca cttgttaacc cacaatatgt cgtcagtgtt
      301 aagcataatg gtggctatag tagagtacgg tttggtacga gtaattatac tttagtagac
      361 agaaataact actcagcaag agatttccat acgcctaggt taaataaact ggtaacagat
      421 gtggttcctg tagaggttac cagtgcagga acagcgcagg gaacatataa aaatgtaacc
      481 aagtttcctg ttttctatag gataggtaca gggacacaaa tatataaaaa tgaacaagga
      541 tattatactc tgaacggtgc ttacagttat cttactgggg ggattgttcc tacgccatca
      601 atttcagatt ggtcatttgt gacaaatgct ggcaacggtg ctcttgcgtc gtatggaact
      661 cccggtgaca gtggttcacc gctacttgga tgggatacaa ctctaaataa atgggctctt
      721 gtgggggtac tcagagcata tgcaggaacc acagggagta ccaactggta tgttgttata
      781 ccaacagtgc aaatcaccgc aaatataaat gctgatactg ataaacctgt taccagtgag
      841 gccgggagcg gtgatattct ctggactttc aacagtgtga caggaaccgg tcagttagtt
      901 cagggagata attcctggga catgcatggt cagaaggact tagatctcaa ttatgggaaa
      961 aatctgacat tttctggagg cggtacaatt gttttagaaa attcggtgac tcagggggct
     1021 ggggcgctga cttttaatga tgactatgtg gtgaaacctc tggatgatca gacctggatt
     1081 ggggggggtg tcattgtcaa tgatgggcac gttgttgact ggcaggtcaa tggtgtgtca
     1141 ggagatgcgc tgcataaatt aggcacgggt acgttgaaaa ttgatggctc cgggattaat
     1201 ggtggaagcc tgaatactgg tgatggcgca gttattctgg ctcagagacc cgatggcgag
     1261 gggaaagttc aggcttttag tagggtatct cttgttagtg gtcgctctat tgtaattctt
     1321 tctgatgaaa atcagattaa cccagataat atttattggg gatatcgtgg aggtacgctt
     1381 gatattaatg ggaatgatct gactttccac aaattaaatg cttttgatga cggtgcaatt
     1441 attacaagta atggcagatt ggccagactg acactgtcct taaatgaaaa aacagcaact
     1501 atttatcatg ggaattttaa gaatgatttg tctgtgacaa ataacaccac caataattca
     1561 ggtgatttta ttgttgatgg tggtataaat ataaatgata attttgagca aaatggtggg
     1621 aaattgtttt ttcaggggca tcctgttcct catgcggtga ctagtcagtc agtagcaaat
     1681 actctcaaaa aactaggaga tgactcagta ttaacacaac cagtgtcatt tacacaggat
     1741 gattgggaga ctcgccaatt cagtttgaag tctcttgttc tgaaagacac cgcgttttat
     1801 cttggacgaa atgcgcagct cactactgat attagtgcag ataactcatc tgtcaccctt
     1861 ggcagtgaag atgtctatat tgatctgaat gacagtaatg aaaagaaagt acaggttatt
     1921 ccgcaacaag gtcaatcaaa agcgacaaag gactcagata aaagtacatt ttatggaaat
     1981 gtatctctga ccaataactc tactttaaat attaatgaat tttttacggg tggaataaat
     2041 agtacagata gtactataaa tatatcttcc cctggtgcaa ctatcagtga gtacagtacg
     2101 ttgacccgtt cggcactgac agtcaccgat aatgcacagc ttacaagcaa ggctggttta
     2161 ctatcggatg acaatgttgt gctcggttca ggggctaccc tctctttgtt gaatgagcaa
     2221 tcgacgatgc cagcgtttta ttcagcggca gcctgggatt tgcaaggttc tggatcaacg
     2281 ttgaatgttg gttccggtac gatgatgagt ggtgatatta ctgccggctc aaaagcgaca
     2341 attaatattg gtgttagtac gaaccaaggt tctccattag gcatctacta ttccggagat
     2401 atcaaagcac ctggggccaa tgttagtatt gaccagacat tgtggaagct taactcgtct
     2461 tctcaaatgc aaaatctggc actatctaac agccagcttg tgatgaccac cgatggtaaa
     2521 accagcagca atattacggt tctcgataag ttaatcgcag agaacaatat tctgtatgta
     2581 aaaccgacca gaccacttaa cgaaatgtct gtcggtgata ttccgttgat cacggcaaaa
     2641 aatggaacca acaatactca ggcgtttaaa actgtgcctc aacagatggg ttttcactca
     2701 atggctcctg atgttgaggt ggtaaatact gacggtgcca cacagtggcg tctgaaagga
     2761 ttcgatgttc agcgtaataa tgctgcactc aatgaaagtc aacgttttat ggatatccgt
     2821 tacagaaact tcttatctga ggtgaataac cttaaccgac gtatgggtga tctacgcgat
     2881 acacaaggag aagccggtgc ctgggctcgg tttatgacca cttctggttc gggtgagggg
     2941 ggattcactg atcgccatat gcatctgcaa attggtgccg acaaaaaaca tcactttgat
     3001 ggaggcgatc tttttacagg tatcatgatg actgttactg acagcaaagc cagtggtgaa
     3061 agttaccagg ggaaaacacg ctctgtaggc ggcggtttat atgcatccac cctgtttaac
     3121 tcaggattgt atattgatgt ggttggaaaa tatgtccatc atcgtgacga ttacacaatg
     3181 caaacattgg gacttggtaa ggcagacgat actgctcact catggtattt aggcgcagaa
     3241 acgggttggc gctatcagtg gaagcctgat gtctttattg aaccgcaggc cgaactggtt
     3301 tacgggacgt tgtcaggcaa tacctttaac tggcaataca acggtatgga tgtcagcatg
     3361 gagcgtaaaa aggcaaaacc cttgattggc cgtactggtg tggagtttgg aaaaacactg
     3421 gatggccgcg actggcaggt tacagcgaag gctggtttaa gttatcaatt tgacctgcgt
     3481 aatactggtg ctaccacgta ccgtgatttt gctggtgagt ccaccgtgta caacggtaaa
     3541 gatggtcgta tgttagccaa tattgggatt gatacacgca ttaaagacaa tacccgtatc
     3601 ggcctgacca ttgagaaatc agcatttggt aggtacaacg tcgataatgc gatcaatgct
     3661 aatatccgct atacattcta atatttagaa tctcacacga aaggtcgttt tatctacaag
     3721 gcccgatatg cgtacgtatc gggccatctt gcgtttgtca tcacactcat atcaatatca
     3781 cctgcttttt tgtaaccttg ggtcatactc atattttgtt aacaaaaagg aaacataatg
     3841 aaactcgtcg gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa
     3901 aagggcataa ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg
     3961 caatttaacc cgttagggaa agtgccggtg ctggtgaccg aagagggcga atgttggttt
     4021 gattcgccga tcatcgctga atatattgaa ttaatgaatg tcgctccggc gatgttgccg
     4081 cgcgatccgc tggagtcgtt gcgagtgcgc aaaattgagg cgctggcgga tggcattatg
     4141 gatgccgggc tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat
     4201 gaattgttac gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg
     4261 gtcgatggca cgctcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgcc
     4321 gtcggatatc tcaatttccg ccgcgttgcg ccaggctggt gtgtcgaccg cccacactta
     4381 gtcaaactgg tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag
     4441 gcttgatgcg cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa
     4501 cgcccttctc cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg
     4561 ttccctctat agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc
     4621 tttgcgtgat acttatggtc acacccgcgt ggtagaattg ctgcgtcaga tgcttgacga
     4681 agcgcgagaa gtgattcgtg atagccagac gctgcccgca tggtgtgaaa actgggcgca
     4741 agaagtcgat gcccgtctga cgaaggaggc gcagagcgcg ctgcgtccgg tgatcaacct
     4801 gacgggaacc gtgctgcata ccaatcttgg gcgagcttta caggcggaag ctgcggtgga
     4861 agccgttgcg caggctatgc gttcgcctgt gaccctcgag tacgatctgg acgacgccgg
     4921 acgcggacat cgcgatcggg cgctggcgca gctgctgtgc cgcattacgg gagcggaaga
     4981 tgcctgtatc gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag
     5041 cgggaaagag gtggtggtat ctcgcggcga actggtggag attggcggcg cgtttcgtat
     5101 tcctgatgtc atgcgtcagg caggctgcac cctacacgaa gtggggacca ccaaccgcac
     5161 gcacgctaat gattatcgtc aggcggtgaa tgaaaatacc gcgctgttga tgaaagtaca
     5221 tactagtaac tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc
     5281 gctcggcaaa gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggttga
     5341 tcttagccag tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt
     5401 cagtctggtg agcttctccg gcgacaagtt gttaggcggg ccgcaggctg gaattattgt
     5461 tggtaaaaaa gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc
     5521 ggataaaatg accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc
     5581 actgagtgaa aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat
     5641 ccaggcacaa cgtttacagg ccccccttgc cgcacattac ggcgcggagt ttgcggtaca
     5701 ggttatgcca tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag
     5761 cgcggcatta acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc
     5821 ccgctggcgt gaattgccag tgccggtgat tggtcgtatt tatgacggac gattgtggct
     5881 ggatttacgc tgccttgaaa atgagcaacg gtttttggag atgttgttga aatgattatt
     5941 gcgactgccg gacacgttga ccacggcaaa acaaccttat tgcaggcgat tactggcgta
     6001 aatgccgacc gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc
     6061 tactggccgc agccggatgg ccgtgtgcct ggttttatcg acgtacccgg tcatgaaaag
     6121 tttctttcca acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg
     6181 tgcgatgacg gcgtgatggc acagacccgt gagcatctgg cgattttgca gctgaccggt
     6241 aacccgatgc tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttaat
     6301 gaggttgaac gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg
     6361 tttatcaccg cagcaaccga aggtcgggga attgatgccc tgcgcgagca tttgcttcag
     6421 ttaccagaac gtgaacacgc cagccaacat agtttccgcc tcgcgatcga ccgtgcattt
     6481 accgtaaaag gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta
     6541 ggcgattcac tctggctgac tggtgtaaat aaaccgatgc gcgtgcgtgc gctacatgcg
     6601 caaaatcagc caacagaaac tgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt
     6661 gatgcggaaa aagagcagat tcaccgtggt gactggctgc ttgccgatgc gccgccggag
     6721 ccgttcacac gggtgattgt cgagcttcaa acccatacac cgctgaccca gtggcagccg
     6781 ctgcatattc accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac
     6841 cttgccgagc tggtgttcga cacgccgcta tggctggcgg ataacgatcg cctggtgctg
     6901 cgcgatatct cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg
     6961 cgtcgcggta agcgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag
     7021 agcgatgccg atgcgttatc tgttcatctg gaacgcggag cggttaacct tgcagatttc
     7081 gcctgggcac ggcagctcaa cggcgaaggg atgcgcgaac tgctgcaaca gcctggctat
     7141 attcaggctg gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt
     7201 ctcgacacat tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgagaa
     7261 cgtctgcgac gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa
     7321 aagatgcgcg aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac
     7381 aaagcgggct tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt
     7441 gacgaaccgt ggtgggtgcg tgatctggca aaagagacgg gaaccgacga gcaggcaatg
     7501 cgcctgacgc tacgccaggc ggcgcagcaa ggaattatta ccgcgatcgt taaagatcgt
     7561 tattaccgta acgatcggat tgtcgagttt gccaatatga tccgcgatct cgatcaggag
     7621 tgtggttcaa cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg caagctggca
     7681 attcagattc tggaatattt tgaccgtatt ggctttacgc gtcgtcgtgg aaatgatcat
     7741 ttattgcgcg acgcattatt atttccggaa aaataaggaa atgattaatt cataataaat
     7801 atgaattaat ac
//
LOCUS       DAZFRE010000167         7698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFRE010000167 DAZFRE010000000
VERSION     DAZFRE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.168"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            282..1031
                     /locus_tag="ACHKUI_004749"
     CDS             282..1031
                     /locus_tag="ACHKUI_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO6600784.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            958..3080
                     /locus_tag="ACHKUI_004750"
                     /pseudo
     CDS             958..3080
                     /locus_tag="ACHKUI_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3250..4302)
                     /locus_tag="ACHKUI_004751"
     CDS             complement(3250..4302)
                     /locus_tag="ACHKUI_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6600785.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4617..5933
                     /gene="shiA"
                     /locus_tag="ACHKUI_004752"
     CDS             4617..5933
                     /gene="shiA"
                     /locus_tag="ACHKUI_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6600786.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            6035..7489
                     /gene="amn"
                     /locus_tag="ACHKUI_004753"
     CDS             6035..7489
                     /gene="amn"
                     /locus_tag="ACHKUI_004753"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6600787.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGRGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2075 a         1798 c         1745 g         2080 t
ORIGIN      
        1 aactatattt attctgctgc aatcaatgca tacataacac aaatatcact caggtacact
       61 actcaaacca cgctgggatt tttcctcaag ttataatcat caccccgaaa atcattcggc
      121 atttactcat taaatagtca ccccataggc ctgtacatgt ttactcagaa atatacatcc
      181 ttttctctgt cataaaccct ctgattaatc atagataaat acttgtgata ccaatctttt
      241 tccttaaagg aacgaattgt tgtgtagaag gagataatat tatgtcaaaa aaatatcagc
      301 ctttgcttat aactcattat atgtcaacat gggtcactat aacggaagca gttgagatca
      361 ctaccaaagc cataaaacaa aaaattactc ctagcgatat ttatcgtcat gccttgagtg
      421 gcaacatcct actatcggtc tatttccagt ctcctgtgat acttaagaag atacaaactt
      481 ttaatggaaa aataaaattc aggcagtttg tgggagacct acttgataaa ctatgcatgc
      541 ttgacaggga cggttttatt tatggtcaaa atttaagact ctgtacagaa gccagatata
      601 tttgtcctgt gcaacaaatt atagataccc ccctgttacg caaactcaac cagtttcgta
      661 cgtttgttcg taatgtccgc ccgggtgatg aactggatgt cccggcacaa gttagtgaaa
      721 aaaatttaac cccgccgccg ggtaacagca gtggcaacct tgagcaacag atagccagta
      781 cttcacagct aattggatct ctgctcgccg aagatatgaa cagcgagcaa gcggcaaaca
      841 tagcacgtgg atgggcctct tctcaggctt caggggtaat gacagactgg ttaagccgct
      901 tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt cagcctgaag aactcccgtg
      961 acggcaaccc tcgaaaatgg cgattcgatg caacaaacag tgaactatgt gccgaacgtc
     1021 acgaatgctg aaatcacgct ggcagcctcg aaggatccgg tgattgccga caataacgat
     1081 ctcacgacac taacagcacc gtcgctgata cagagggcaa tgcgatagcc aacactgagg
     1141 taacatttac tctaccggaa gatgtgaagg cgaacttcac gctgagcgat ggcgacaaag
     1201 cggttactga tgccgatggc aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc
     1261 ataccgtgac agcatcgatg gttggcggta agagtgagca gttggtggtg aactttactg
     1321 cggatacgct cactgcgcag gttaatctta acgttaccgg ggacaatttc atcgccaata
     1381 acattggaat gaccaggcta caggcaacag tgactgatgg aaacggcaac ccagtagaag
     1441 gtataaaagt taatttccgc ggaacctccg tcacgctaag cagcaccagc gttgaaacgg
     1501 atgatcaggt tttcgctgaa attcttgtga caagcaccga ggtcggacta aaaacagttt
     1561 cagcctctct ggcagataaa cctactgaag tcatctcgcg attactgaat gccaaggtag
     1621 atgttaattc tgcgacgatt accagtcagg agatacctga aggtcaggtc atggtcgcac
     1681 aagacatagc agttaaagct cacgttaacg accagtttgg caacccggtg acgcatcaac
     1741 ccgcgacatt cagtgcagcg ccatcctcgc aaatgatcat cagccagaat acggtctcta
     1801 ctaatacgca gggagtagcc gaggtcacca tgacgcctga aagaaacggc tcgtatactg
     1861 taaaagcatc cctagcgaat ggagcttcac ttgagaaaca actggaggct attgatgaaa
     1921 aactgacact cacgtccagt ccgcttatcg gtgtcaatgc tcctaaaggc gctactctga
     1981 cggcaacgct aacctctgca aatggcactc cagtggaggg tcaggtcatc aactttagcg
     2041 taacgctaga aggtgcgacg ttaagtggcg gaaaagtgag aaccaactct tcaggtcagg
     2101 ctccagtcgt tctgaccagc aataaagtcg gtacatatac ggtgactgca tcgttccata
     2161 acggcgtcac aatacagaca cagacaaccg tgaaggttac tggcaaccca agcaccgcac
     2221 atgttgctag ctttatcgct gatccgtcga ctatcgccgc caccaacagt gatttaagta
     2281 ccttaaaggc aacggttgag gatggcagtg gtaacctgat cgaaggtctc actgtgtact
     2341 tcgccttaaa aagcggctcc accacattaa cgtcattaac agcggtgacc gatcaaaacg
     2401 gaatcgcgac aacaagcgtg aaaggtgaga taacggggag cgtcactgta agcgcagtca
     2461 caagcgcagg tggaatgcaa acagtagata tatcgctagt ggcaggccca gcagacgcct
     2521 cgcagtccat ccttaagaac aaccagtcat cactgaaagg ggactttacc gatagtgctg
     2581 agctacatct tgttctgcac gatatatcag gcaatccgat caaagtttct taagggatag
     2641 aatttgtgca atcaggtaca aacgtgccct atatgaaaat tagcgcaatt gattacagtc
     2701 aaaatatcaa cggtgattac aaagctactg ttacaggagg cggagagggt atcgcaacgc
     2761 tgatccccgt attgaatggt gttcatcagg cgggtctgag taccacgata gaattcatta
     2821 gcgcggaaac cagaccaatg acgggtacag tgtcagtgaa tagtgcaaac ctaccgacag
     2881 cttcattccc ttcgcaggga tttaccgggg cgtattatca gttgaataat gacaactttg
     2941 caccaggaaa aacggcggct gattattcgt tttcaagctc ggcctcctgg gtaggcgttg
     3001 atgctactgg taaagtgaca ttcaaaaatg acggtgatag caatactgta ataatcacag
     3061 cgcccccaag atccggtggt gctatttacc aaacagttcc tcctgaatcg cgttcggtgt
     3121 gactatgatc attttcggtg agtattgctt tgatccaaag gctggttttc tgtgatgatt
     3181 gaccagcctc agagtattga tgacaccacc gttcataaca gatcggcatt actatgcata
     3241 agtactgcct caaattctcg ccaaaacact ttttaatgcc tttgcgaatt catccagact
     3301 tatattgtca attttttgcc caacgacgac tgactccgaa acatcggcca ttgcgggcca
     3361 tcgcgttttg taatcagcat aaattgcaac aacaggggta ttataggcac ttgccatatg
     3421 taatattgag gtgtcaggag ttattgcaat ataagcatct tttatgattg ctgctgaacg
     3481 tttaatcgag ggtgataagg ataaacggta gacattatta taacaatcca ctaatgccct
     3541 ggctttatct tcccctttgg gtccataaac gatcactatt ggaatatctg tttcactttg
     3601 aatttttgct attagatttt ctgcaatcga taatgaaaat gtacgttcct gcgagctacc
     3661 gtctaaattg agcgcaatgt aagatcctaa cgatcgcatt tcctcgcgta cctcatcgag
     3721 tacatcatcg cttaatggca attcatatat tgacctgact gccggaaaac ccgcctcacg
     3781 catcataaat gcccatgtca tgggtacagg agtccggaga ttctgatcca tccgggacgc
     3841 gttcttacac aaaggggaat agcacttcat ggttaaaccg acgacctgga aattcgtttt
     3901 tgctttcaac tgactgataa atatcatcgt tttcaggttc tttttacgca tcgcctctat
     3961 acaaagatcc ggcgtaccgt attgcagtgt tatgttcttc gctaaatctt ttatttcttt
     4021 taatgttgcg tgatcctgca tagtcattgt gactaatgtt aatttagtct gttcaagttt
     4081 gagcgcatta aagacttcta aattaattgt cgacgtaaca attaatagat gcttaatttt
     4141 atgcaattca agcgcccgaa taacaggaaa gatggccata gcatcgccaa tctgatcggg
     4201 aatatgtatg acaacaaagt ctgttttttc aatattaaaa ttataagctt tataatcgta
     4261 gtaactaaat gcaatacgtc tcaacaatga tactaaaaac ataactaacc tcgcctccct
     4321 actggttata atgcaatgca gtctatcaga atcatcaggg tgcaattttg tgcatatgcg
     4381 gacttttatg tttcatatct cgaacctgtg ggccctctgc ttaatcctta aacaacacca
     4441 gcaactcctg cgctttcatc ttccatcgaa tttttcatgt tgccgctaat cagccataaa
     4501 atcatttgca gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc
     4561 aatgtgagtt accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg
     4621 actccacgct catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac
     4681 gagctgcgtt aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg
     4741 gcattaccgc cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg
     4801 gaacgctcgc cgcatttgcc acctttggtg tcggcttcct tttccgaccg ctcggcggtg
     4861 tcattttcgg tcactttggt gaccggctgg gacgtaagcg catgttaatg ctgaccgtct
     4921 ggatgatggg catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt
     4981 ggtgggcacc tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg
     5041 aatggggagg cgcagcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt
     5101 acagtagcgg tgtacaagtt ggctacggag taggtttact gctttcaacc ggactggttt
     5161 cattgatcag tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt
     5221 tcctgtttag catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt
     5281 ccgcggaatt tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta
     5341 tcgaagctct gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg
     5401 agttgctgac gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg
     5461 ggttaccgcg cgaacttttc cttaatattg gtttgctggt cggtggatta agctgcctga
     5521 caattccctg ttttgcctgg cttgccgatc gttttggtcg gcgcagggtt tatatcacag
     5581 gcgcgttgat cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta
     5641 ttttctggat agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt
     5701 gtgtgcaaca accgatgttt accgaaatgt ttggtgccag ttatcgctac agtggtgctg
     5761 gagtcggtta tcaggttgcc agtgtggttg gcggtggatt tacgcctttt attgccgctg
     5821 cactcatcac ttactttgcc gggaactggc atagcgtcgc tatttatttg ctggctggat
     5881 gcctgatttc cgcaatgacc gctttgttga tgaaagacaa tcaacgcgct taatagcctg
     5941 gcgaagatca tccgatcttc gccttacact tttgtttcac attcctgtga catactatcg
     6001 gatgtgcggt aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac
     6061 cccagctcag gcactggata aactcgacgc gctgtatgag caatctgtag ttgctttacg
     6121 caacgccatt ggcaaatata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca
     6181 aggtcttttt gtctatccat cactgaccgt aacctgggac ggtagcacaa ctaatccccc
     6241 caaaacgcgc gcatttggtc gttttactca cgcaggcagt tacaccacca cgattactcg
     6301 ccctactctc tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg
     6361 tgcgcatatc tctgtgcaac cctcgcagca tgaaatccct tatccttatg tcatcgatgg
     6421 ctctgaatta acacttgatc gctcaatgag cgctggttta actcgctact tcccgacaac
     6481 agaactggcg caaatcggcg atgaaactgc agacggcatt tatcatccaa cggaattctc
     6541 cccgttatcg cattttgatg ctcgccgggt tgatttttcc ctcgcacggt tgcgccatta
     6601 taccggtacg ccagtagaac attttcaacc gttcgtcttg tttaccaact acacacgtta
     6661 tgtggatgaa ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat
     6721 tgccctttct tgtgctggcg ggaactggat cactgccgaa accgaagcgc cagaagaagc
     6781 catttccgac cttgcatgga aaaaacacca gatgccagca tggcatttaa ttaccgctga
     6841 tggtcggggt attactctgg tgaatattgg cgtgggaccg tccaatgcta aaaccatctg
     6901 cgatcatctg gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt
     6961 acgtgaaagc caggccattg gagattatgt ccttgcacac gcttatttac gcgatgacca
     7021 cgttcttgat gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg
     7081 tgcgctttat gacgctacca agttagtcag cggcaggcct ggtgaggaag tcaaacagcg
     7141 gctgcgtaca ggtactgtgg taaccacaga tgacaggaac tgggagttac gttactcagc
     7201 ttctgcactt cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat
     7261 tgccgcgcaa ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa
     7321 accgttacat ggtgagatta agcttcccgg ccaggctaac cgtttttatg aaggcgctat
     7381 ttccgaacac ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg
     7441 actgcattca cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttatgaa
     7501 ccttcttatc agaaatagat gtgaaggatt aagtaagaca tctggcaaat agcctgcaat
     7561 aaacgcgggc tgtttgcttc cttgggcaga tgcgagtttt attatcgtct taatgatttc
     7621 cacatattaa aagcaagtat gctttcaaaa cacaattata aaaaatcccg ccaacaacat
     7681 tagtttttat aaaattaa
//
LOCUS       DAZFRE010000168         7688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFRE010000168 DAZFRE010000000
VERSION     DAZFRE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.169"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(1..541)
                     /gene="papA"
                     /locus_tag="ACHKUI_004754"
                     /pseudo
     CDS             complement(1..541)
                     /gene="papA"
                     /locus_tag="ACHKUI_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
     gene            complement(749..1062)
                     /locus_tag="ACHKUI_004755"
                     /pseudo
     CDS             complement(749..1062)
                     /locus_tag="ACHKUI_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
     gene            1467..1700
                     /gene="papI"
                     /locus_tag="ACHKUI_004756"
     CDS             1467..1700
                     /gene="papI"
                     /locus_tag="ACHKUI_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HFO6600788.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
     gene            complement(2354..3477)
                     /locus_tag="ACHKUI_004757"
                     /pseudo
     CDS             complement(2354..3477)
                     /locus_tag="ACHKUI_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(3080..3196)
                     /locus_tag="ACHKUI_004757"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3595..3696)
                     /locus_tag="ACHKUI_004758"
                     /pseudo
     CDS             complement(3595..3696)
                     /locus_tag="ACHKUI_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            complement(4217..4966)
                     /locus_tag="ACHKUI_004759"
                     /pseudo
     CDS             complement(4217..4966)
                     /locus_tag="ACHKUI_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            5515..5775
                     /locus_tag="ACHKUI_004760"
     CDS             5515..5775
                     /locus_tag="ACHKUI_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO6600789.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            5817..6377
                     /locus_tag="ACHKUI_004761"
     CDS             5817..6377
                     /locus_tag="ACHKUI_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6600790.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKMLDTRT"
     gene            6417..6845
                     /locus_tag="ACHKUI_004762"
     CDS             6417..6845
                     /locus_tag="ACHKUI_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HFO6600791.1"
                     /translation="MKKFINVYIKSDYDIVILRLSVIIIFILFGTYKWFDFEVMALKP
                     LISPTWLNILYIMFGVHGGSYFLGIVETITFLALSIGFFRPAAGILGDIIVIVTGLVT
                     LSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKNYLNIKN"
     gene            complement(6991..7201)
                     /locus_tag="ACHKUI_004763"
                     /pseudo
     CDS             complement(6991..7201)
                     /locus_tag="ACHKUI_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(7344..7457)
                     /locus_tag="ACHKUI_004764"
                     /pseudo
     CDS             complement(7344..7457)
                     /locus_tag="ACHKUI_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7554..>7688
                     /locus_tag="ACHKUI_004765"
     CDS             7554..>7688
                     /locus_tag="ACHKUI_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6600792.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     Q"
BASE COUNT         2120 a         1879 c         1566 g         2123 t
ORIGIN      
        1 gttgcgactg cagaaaaagc accctcagtg acttgggcat tattggctga cgacttctta
       61 acaatcgcag tataatgcag cacattatca ccatctttca gggtattagc atcaccttct
      121 gtgccatcga aagaaacgtt tttacctgca gcctgaacta caatcgcagt acctgtcccc
      181 ccgctggtat ctaactcctc attatgacca gaaacccttg gaccactaaa agccagcttc
      241 acagtccctt tttttgctgc cgagactcct cccccccctt aaatgaagtg atatcacaat
      301 taaccaattc aatatctaaa ttcattggtt tggatacgcc tcctgcttga aggaagcttt
      361 ttgaaagctg tccaaaatca atagactgat cagctgattt ctgagaaatg ctgcatggag
      421 catcaacaac agttccgtta aaggttactc taccctgccc ctgtggagta gttagagcag
      481 catttacacc aaaagacacc actgccatag ctaccgcacc ggcaataacc gacttaatca
      541 taaataacaa cctctttttc attactgaac acaccagaga tatctggcaa tttaaaatga
      601 cccgattacc tttaataacc agatcaatac aaataatatt ctcgtatttt cagggcaggt
      661 tatttatcga cagaatactg atataacctg tctgctttct gacaagggct tcttttttta
      721 tcttccagaa actccggaat gccataattt agtcaaatgc cgatgactca tctgtataat
      781 aaggtgcaag ccttgctgca agagcattca gccgtataag tctccccagt gttgtactga
      841 aatacccatt attcatctgg tatttctcgc agacctcctt acgggagtgc ccacctacca
      901 gatagtcctt catagccaga atgaccctgt cactgtgaat agaagaaata cctatcagta
      961 aaaaaaatgc atttcagaca tagagccgag caacagcacg ctctcgcgta tattcagcaa
     1021 aaacgcattt cctgaccgac tgatgacttc atgatgcgcc atgtttcccc cttctgtcgg
     1081 gcccctggat atatgcttcc ggcccggaaa tactcttcac gcaataagtt aaatttagtt
     1141 ttttatgttg taaatattga tttaaacgat cttttagccc acaaaacaaa attaaaccag
     1201 aataaaattc taacaacaag aatacactca gataaaaaca tcatggtgaa ttgaatttac
     1261 agcataaaag atcgtctaaa tgcattttta cggactttct tttcacagaa aaataacaca
     1321 caaaatgtga tacacatcaa ataaagccaa caaaaagcaa attcactaaa ttgcacaaat
     1381 aaagcaaaca cagagaataa aaataccttt ccgttgataa cagagagcat gatttttatt
     1441 attcgaagaa actttgttac agtgagatga gtgaatatat gaagaatgaa atactggaat
     1501 ttctgaacag gcataatgga gggaaaaccg cagaaattgc ggaggcgctg gcggtaacgg
     1561 attatcaggc acgctattac ctgttattac tggaaaaaga aggtgtggtt cagcgctctc
     1621 cattaaggcg aggcatggca acatactggc ttctgaaagg agagaagcag gcggggcaga
     1681 actgttcttc cacaacttaa taactcaatc tgtgggcatg cttcactgaa acagataaaa
     1741 gttacatcag agtgcagatt caattttgca gcattcatca caaaatagaa atttagtatt
     1801 ttatgtcaga atacttcaca tgataataaa aacctgtctg gatttactgc atggccagac
     1861 attacaaaat acaaacgcca taaacttcat tcagccttct tgctgtccgg taacggaggc
     1921 tgctttttcg ttacaccttt ccccggcagc atcagtagca gtaatttata gattctacga
     1981 actgagactg tctgtccggg gagaacgtac acagcccgac acaatgtacc agtcaataat
     2041 atccggtttc tgattccctg cctgacagac cactctctgg caactttgta ttcagttcat
     2101 ctactactgg agacagattt ataaaatcca actccacgat ctttgaccgt ccgcatcccc
     2161 tactctctgt gctgcggtca tcggttaatt ccagcgtccg tccgttacga tcagatatta
     2221 ctgaaccgcc ccgggtttcc tggagagtgt tttatctgtg aactcaggct gccagatcat
     2281 tgtttccgat ggaagcataa taagcttttt ctgcttctgc cggaggagta tggcccagcc
     2341 ttcccagcaa tcatcgattg ttataccagt ccacccacgt gagtgtggcc agttccactt
     2401 ctgcacggtt tttccagctc ttacggtgta ttacctccgc tttgtaaaga ccattgatgc
     2461 tctccgccat cgcgttgtca tacgagtcgc ctgtactccc tgttgatgcc agcagttttg
     2521 cttcttttaa tcgctccata taggccagtg acacatactg agagccttta tcgctgtgat
     2581 ggatggtgcc ggacggacgg cgggcccaca acgcctgctc cagcgcatcc agcacgaatg
     2641 tcgtttccat agacgatgag acccaccacc ccacgatgta tccggcaaac acatcaatga
     2701 tgaacgccac atagacgaag ccctgccatg tgctgacgta agtaaaatca gccacccaca
     2761 gctggtcagg acgttctgcc acgaactgac ggtttacgcg gtcacctgca gcaacggttt
     2821 tccggctgac ggtagtgcgg acctttttac cccggagaac accggcaagt cccataaccg
     2881 ccatgaggcg cgccactgta catctggcca ccctgattcc ttcccgtaac aactgacgcc
     2941 agactttacg cacaccgtac acctgatgat tttcatcgta tacgcgctgt atctctctct
     3001 tcagccagtc atcgcgctga caacgggcac tgcgtttatc aggatgatgc cgctgctgct
     3061 gacaatggta atacgttgac ggggcaatat gctgttcact gcataccggt ccgaccccgt
     3121 actgctcacg cagcttatcc agcagtggca ttattttttc cagaggcggt cgaactccgc
     3181 cttcgcaaaa taagcggaag cctggcgaag gatatcgtta ctgcggcgca gttcacgatt
     3241 ttcacgttcc agctctttca gacgctgacg ttcagcggtg gtgagcccac catcaccgcc
     3301 cccggtatcc cgctcatgct ggcgaaccca gacacgcaga gtctctggcg tacagccaat
     3361 ctttggagct atggaacaaa ttgccgccca ttgtgagtca tattcgtcct gactttccag
     3421 aaccatacga actgcccgtt gacggacttc gggggaaaaa cgtgtatttt tagtcatcct
     3481 gtttacctct ttctcaggga atttagtctc caggattccc ggggcggttc acagtaatca
     3541 tgccgggtct ggctggctaa caccccatta taaggggtac gaataaagta acggggcttc
     3601 acgcagggat ggacagattg ctctgatata cagccgacga cgtcccgctg caacatcatc
     3661 acgcagatgc ttcattttca gatttgggac ctccttttga tttctcgttt aacttttgct
     3721 gttaccagac tttaatctat tttaacaatt caagaatttt ttataaaatg ctcaaagatg
     3781 aaagcgttac tgaaccaacc agcttggctg atgttttcta tagataaaaa gatcatcagc
     3841 aaaaactgat agcctgaatc acctttacta ccaatcatgt atgaagaaaa cacgcacaaa
     3901 caattgacaa tgattatcat ttgcattaaa aggattatgt agtattttta tttcatgagg
     3961 aactataccc gccagcaatc tgagtagcga aagctgttca tccccaatat ttttgctggc
     4021 gggttctttt ttctactgcc cttctcctca gagggagcat tccattcttt ttttcattaa
     4081 tggcgagggg gcgggacaaa aagccggttc tttatcccta aataacagca tgatttcagt
     4141 ccatgcaaaa tctgatttac cagctcacat aataacctga tgatacatgg gttttccctg
     4201 atcatggtgg gaaggctcag aacgccatat tcagtttccc gtagtggaac atcacgccca
     4261 gcttaaagcg ctcccgattt cggtatcccc tggcttttat cctcagtagc ctgatcttac
     4321 tgttaagcgc ctccgcgttt ccgtttgaga cacggtgtcg catggcattc aggatcccgt
     4381 aaagcctttt tcctatcgtt ttcgcggcgt ttttcatcat aggaacgtca ctgttggccg
     4441 ccagagccag ccatctctcc cagtcattac gtctttcctc actccatggc ctgttccaga
     4501 tatcctttgc cagctctttc agcgcccagc actggcttgt cagcttcatc tgtgcacgca
     4561 gccatatcag cttttcctgc ctggatttgg tcatccattt atcgctgtac tgccacagga
     4621 agcgggtccc ctttgcctga cgccaacttt caacagggag gtgcggatgt tcattctgac
     4681 gggttttatc aaccacttcg ccaagttgct ttgctacatg gaagcggtcg aaggcgattt
     4741 tctcaaccgc acagggtaag tggatacgcg ctgctcttat atagcctgca ttcatgtcca
     4801 ttgagagcgt tttgatagcc agcaactgcc cgtcagtaag cgtgcgaaga tagccggcga
     4861 ggctctctgt gccacgatcg tccgttaagg ccagcgcacg accatcgcga tcggagatca
     4921 ccgttatata acgatgtcct tttttaaagg cgacctcatc cacattgtca gcaccagtga
     4981 tataagacgg atagcaaagc gcacgcgctg tttgtcaccg tgaaaaacga cggtgcgacg
     5041 gcaaaagcca ataacaacga gagcgtcgtg cgcattcctc tgcactgaca gttgattctt
     5101 agaacgtaga ggcctggatt gctgccaggc ttttattgca ctaccagcta cggctaaaag
     5161 ataagcgtcc cgggaagtac atagcgctta caaaatatcc gacatgaagc atactggaaa
     5221 cgaggcgtat tgttattgtt gttcatattg gcttccgtgg cggaaatatc agggggtagc
     5281 tacatatccc ccatgcagcc aaggcaacga tgttaactct cgtcacctgt tcctttcgac
     5341 agagccaacc tcgcagaggg ccagatctta caacttcacc tcagtgatac aaaacacaaa
     5401 aatacaaaac aatttgatac aaatcatccc tcttatttta aataaatcca tcttgcaagc
     5461 ctaccaatca gaaggtagca tgtgtagtat cgcaaacata aagagaaatt gctcatgaat
     5521 tatgttatct gcactaccat tgtcgccctt ggtctcattc tgacaggcta cgcaactgct
     5581 gcagatgaaa ttaatcgtca ggacgttgtt ggtttgatgc cgtcagacta tatttctgtt
     5641 ggtaatctat atacaccaga agaggtggca actgcgctta aagacaaagc ggagcaggca
     5701 ggaggttctc atttccgaat cacttcattg ggaggaaaaa ataaacttga tggaactgcc
     5761 attatctatc gctaaaatat gtataatagt ccctttaata cataacaggt aaagccgtgt
     5821 ctacttatga gaatatactt cgcataacgg atactctgat ccagcaacgc ggatttttgg
     5881 ggttcagtta cgcagatctg gaaaaagaaa ttggtatcag aaaagccagt attcatcacc
     5941 atttcccgag gaaaactgat cttggtatag cctactgcca atacaaaaca gaagtatttg
     6001 atagacttaa tataactctc cataatgttt cttcaggtgt acaacgtctc aggacatatc
     6061 ttgatgcatt tgctggttgt gctgaaagag gggaaatgtg cggtatttat gccatgctat
     6121 ccgacagcca tcagttttct cctgaattac aggaggctgt aagtcaactg gcacatcagg
     6181 agatacagat gattaaagat attatcacct ccgggcagaa tagtggagag ttcaaaacag
     6241 ttcttcttcc ggatgagctc gccgttatag tctgttctac actaaagggg gcactgatgt
     6301 taaatcgact tccaccacat gatatctatt caggaaccgt taacgcttta attaaaatgc
     6361 ttgatacccg tacttaatcc tttttagtgc atataatttt ccacccggag aatgaaatga
     6421 aaaaatttat taatgtttac attaaatcag attatgacat agttattctt cgcctgtctg
     6481 ttattataat atttatactc ttcgggacct ataaatggtt tgattttgaa gtgatggccc
     6541 tcaagcccct gatttctccc acgtggctga atatcctgta cattatgttt ggtgttcatg
     6601 gggggagcta tttcctcggc atagtagaaa ctatcacttt tctagcactg agtatcggct
     6661 tcttcaggcc tgctgctggc atactgggag atattatcgt tattgttaca ggtttagtca
     6721 cactaagtct gttaccacaa ctgggaaaag ttgacagttt tgtcatcaaa gatgtgctgc
     6781 tgttaggtgc tggtagtgtc ctgctgaaat atgatttgaa aaattattta aatatcaaaa
     6841 actgattttt cacttcggtg atgaacgttt gcgtattttc atggaaagag attacctaat
     6901 ccccctcgtt ctccttcatg ttaatacgca acgttatgga aaacaaaaat accctgagta
     6961 aaaaggtccg taggcaggcg agtactttta ttttccgcta gttttacttt cccatcgaag
     7021 gccccataca tcactacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     7081 ggataatacg gaaacgcact gcacaggact ctgcggttta cgtcgccatt caatcccata
     7141 agcttcaagt gatgtcttcc ctcagtgctg ctaattcatc actgtcagat ttatgaacca
     7201 tattcgttac ctgtatccga accactgaag caccacagca gaaattcggg tcccctacac
     7261 tgattacagc gccccctctg ctgcctatta actcaagcac ctcccaacaa cttacggaaa
     7321 cttaattcta atacagctgt aattcagttg tgtcgtggtc ggccgcggtt acttttttcg
     7381 ggaagtctgc tacgcatcag aataataccg tgttctttct gatcctgttt agccaggtag
     7441 ctaagggcgt aacgtaaacc gttagactgg ccccctgaat ctccagacaa ccaatatcac
     7501 ttaaataagt gatagtctta atactagttt ttagactagt cattggagaa cagatgattg
     7561 atgtcttagg gccggagaaa cgcagacggc gtactacaca ggaaaagatc gctatcgttc
     7621 agcagagctt tgaaccggga atgacggtct cccttgttgc ccggcaacat ggtgtggcag
     7681 ccagccag
//
LOCUS       DAZFRE010000169         7578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFRE010000169 DAZFRE010000000
VERSION     DAZFRE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.170"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            417..935
                     /locus_tag="ACHKUI_004766"
     CDS             417..935
                     /locus_tag="ACHKUI_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6600793.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPLEA"
     gene            1156..3114
                     /locus_tag="ACHKUI_004767"
     CDS             1156..3114
                     /locus_tag="ACHKUI_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6600794.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIPQRYVTGVVAGFGMQENNGWQMRYHLCIEPPLWRC
                     GLRQNFRIFQQQDIRTISAMLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDTSAGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGESLNGQLEQYEIFDYPGRFKDEQHGKDFTLY
                     QMESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGDQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRQQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYHGMTEESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRT
                     YHEDNRSPGSLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEEIYLHAQKNMQVVVLN
                     SKDKRVNYDRAVSIGHDEFLVVANDRKVTVEGKQDHKTTKDHVSLTEGNQGLEVKGDL
                     AQKISGALGISVQGDIVLQSDSKISLRVGGSFVVIHSGGVDIKGAKINLNGGGSPGDV
                     ILPMRPVILKAAAGSGSMFVAHCPKEDK"
     gene            3114..3953
                     /locus_tag="ACHKUI_004768"
     CDS             3114..3953
                     /locus_tag="ACHKUI_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO6600795.1"
                     /translation="METAPVQYAIVDGAVEEGLLDFLNEVNPPHCCLYAEPIQPDLVA
                     LAPYLVEVVPEVEAWLRDKASPWGIYLTSESSMRELQQHFRRYLWVRIPEQEKPVLMR
                     FYDPRNIWVLAEVLTPRQLLFFINPVRQLSTRYGEECREDNFSSVRPAETMNIRAERP
                     SQLMLSYRQYSQLERKARDNYLDTLSVFIEENAEKEGWDDSSKAESSRILAEDYFSFC
                     QSLNIADDRSVRTMTLILLKKNIIDLRYIPDDWYELFSNQSYPGHIRVHELAQQELGF
                     IPQ"
     gene            3975..5525
                     /locus_tag="ACHKUI_004769"
     CDS             3975..5525
                     /locus_tag="ACHKUI_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600796.1"
                     /translation="MTSPSSTAGKPCVECGIPDPCIFDVISDFPEAGEKHFWSKEGMA
                     RFKLLDDGFGCDGIITITYQCDNSGTHEAWLEEESENSRKILQPFGKPNNVTLYYEGF
                     KGGMNIIDAFHSPWEYLSSITMPHDVLYEPAHYTVRIDSCYEHNSYVEIKVYPSLEIR
                     FTTGLSYEISSSQQTRAVKDRRDERRKAREAMEDTKPKDKNKLRSGWTNTTAEFELFH
                     KTSLTVELGLKVANVEYTHKYVEEIKKLRQIKQLEQMSRIDALIKSINKYFAPDPDNK
                     DGTRKYALFSAKIEPVTLGISYAYQYTDIKDGPCHFYGLYGAPFFKASFRFDIISFIC
                     AYCKMEDLVNRCREYLRKHGTSVECYIEISPEVNLDLGAVYSQKDKAWEFNTKESNIK
                     LGLKGEVSATFETNVFVVKLKSEVGASIEAKAGFGFDKRDDGMDLVLFHDGVTGAFKV
                     KVDISRGDKDRKENSEKPEKENELKWQLCKPLEVDDSPLRINLYGKENTVEKKVTPVT
                     QWQIGTDY"
     gene            5614..6579
                     /locus_tag="ACHKUI_004770"
     CDS             5614..6579
                     /locus_tag="ACHKUI_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600797.1"
                     /translation="MGTGLYSSDNHQKIRSRFNIQESYCAIKTNGVLGFSNRKDVLRE
                     NGDSTGTTSSSTNAMMLMENGENEISLEIGALRWFSDKPASTEERGHFSQKAGCSLDL
                     VRFVKQEETILSSIKVTINQQGIPEAQPDSMHPVIRKEILAEQAEPGFIDPDYFNETY
                     FPKGMKVYQFTQKVSVAGLPEWAWTRATPFTGSDEQLQKLKAAYIEMAAIISSGDRAR
                     LKASHKEALKAWSVTTGDSEDEILLSLYPKEEIEGRKARMQPIRWGDYAIRVMNNGRM
                     VQLYNKSKPTYSPLTYRFIDEDGDEVMGYYAPVFSLIDGQFIPVT"
     gene            6691..6975
                     /locus_tag="ACHKUI_004771"
     CDS             6691..6975
                     /locus_tag="ACHKUI_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO6600798.1"
                     /translation="MPLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLT
                     PHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
     gene            6994..>7578
                     /locus_tag="ACHKUI_004772"
     CDS             6994..>7578
                     /locus_tag="ACHKUI_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO6600799.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRRNFRIFQQQDIRTISAMLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGE"
BASE COUNT         2079 a         1545 c         2108 g         1846 t
ORIGIN      
        1 taataagttt tatgtatggt tttataaata aattaattaa ttgctagaaa tacatcataa
       61 gaaattcctt taaggaatat cttaattgcg tgccggaacg attgtgaagt aagtgttgta
      121 atgattgatt tttgaagaaa ttgtaggttt ttatgggggt aaggatacgt tatggataat
      181 tctactgttg atgttgatat atttttagct tatgtgatcg tgttctgaaa ttgactaaaa
      241 atatatttaa acaccataaa agagtttttc ggtaggtgct aattacgtgt gcctgtaact
      301 cgaataaaga aaagggtgtg gttcctgatg tgagccgcat cacactctaa cctgtcgggg
      361 attaacgacg ggttgttcgt aaaacagcag ttgataattt cacaaggagt tcataaatgc
      421 caaccccatg ttacatttca attacaggtc agacccaggg gaacatcact gccggtgctt
      481 tcaccgcaga ttctgtcggc aatatctacg tgcagggaca cgaagatgaa atgctggtgc
      541 aggagtttct gcataacgtt actgttccaa ccgatccgca gtctggtcag ccttccgggc
      601 agcgtgctca caaaccgttc attttcaccg tggcgctgaa caaagccgtt ccgctgctgt
      661 acaacgcgct ggcctccggt gaaatgctgc cgaaagtgga gctgcactgg tggcgtacct
      721 ctgttgaagg caagcaggag cactacttca ccacacgtct tactgacgcg acgattgtgg
      781 acatgaacct gcatatgccg cactgccagg accctgcaca gcgtgagttt acgcaattgc
      841 tcgcagtatc actggcctac cgtaaggttg agtgggagca catcaaatcc ggtacttctg
      901 gtgccgatga ctggcgtgca ccgctggaag cataaatctg agtcaacaac atcctgcctc
      961 cgtgcaggat gttgtttttg tacgtgtaaa aaatgagtgt cagtaaggag ttacgctttc
     1021 atgagggaga tggtggatgc cggaaacccg ggcggtagcg tacctgaaaa tgtaaagatg
     1081 agtgtaacgc tgcactggta ccctttgctg catggtggga atcatttggt ccgtgaatga
     1141 atcagggagg tcattatgtc cttaaaaggt cttcgcttta cgctggaggt tgacggccag
     1201 gagccggaca cctttgcagt ggtgaatttc aggttaatcc agaaccagtc ttatccgttt
     1261 gtgatgagtg tggatgtcgc cagcgattct tttatgcaga cggcggagat gctgctggag
     1321 aagaaggcaa cgctaaccat ctggcagggc gtcatcccgc agcgttatgt cacaggcgtg
     1381 gtggcgggct ttggcatgca ggagaacaat ggctggcaga tgcgttacca cctgtgtatt
     1441 gagccgccgt tatggcggtg cgggctgcgg cagaacttcc gcatcttcca gcagcaggat
     1501 atccggacga tatcggccat gctgctgaac gagaacggcg tcacagagtg gacgccgctg
     1561 ttttatgagg accatccggc gcgggagttc tgtgtccagt acggggaaag cgacctggcg
     1621 tttctggcgc ggctgtgggc ggaggagggg attttcttct ttgagcggtt tgcggcggac
     1681 agtccggagc agaagctgac gctgtgcgac gatgtggcgg ggctgtcaca ggcgggggag
     1741 tttccgttta acccggacac atcggcagga gcggagacgg agtgtgtcag tatgttccgt
     1801 tatgaggcgc atgtccgccc gtcgtcggtg cagagccagg attacacgtt taaggtgccg
     1861 gactggccgg ggatgtatga gcagcagggt gagagcctga acggacagct tgaacagtat
     1921 gaaatatttg attatccggg aaggttcaag gatgagcagc acggcaagga tttcacgctg
     1981 taccagatgg agagcctgcg cagtgatgcg gaaaaggcca cagggcagag taattcgccg
     2041 aagctgtggc cggggacgcg gtttacgctg acggggcatc cgcagaagat gctgaaccgt
     2101 gaatggcagg tggtacagag cattctgtca ggagaccagc cgcaggcgct tcatggcagc
     2161 caggggagag gaaccacgct gggaaatcag ttagaggtga taccggcgga ccggacgtgg
     2221 cgtccacggc agcaaagcaa accgaaggtg gacgggccac agagcgccat tgtcaccggt
     2281 cccgcaggag aggaaatctt ctgcgacgaa cacgggcggg tacgggtgaa gtttcactgg
     2341 gaccgttatc acgggatgac ggaggagagt tcgtgctggg tgagggtgtc acaggcgtgg
     2401 gcggggccgg ggttcggtaa cctggcgata ccgcgtgtgg gtcaggaagt gatagtggac
     2461 ttcctgaacg gggacccgga ccagccgctg gtcatggggc ggacgtacca tgaggacaac
     2521 cgttcgccgg ggagtctgcc ggggacgaag acgcagatga cgatacggtc gaagacgtat
     2581 aaaggaagcg gttttaacga gctgcgcttt gaggacgcca cggacaagga agaaatctac
     2641 ctgcacgccc agaaaaatat gcaggtcgtg gtgctgaaca gcaaggataa gcgggtcaac
     2701 tatgaccggg cggtcagcat cgggcatgat gaattcctgg tggtggcaaa tgaccggaaa
     2761 gtgacggttg aaggtaaaca ggaccataaa accaccaaag accacgtcag cctgacagag
     2821 ggcaatcagg gactggaggt gaaaggcgat ctggcacaga aaataagcgg cgcgctgggt
     2881 atcagcgtac agggcgatat tgttttacag agcgacagca aaatcagtct gcgggtggga
     2941 ggttcatttg tggtgatcca ttccggcggc gtggatatta agggcgcgaa aatcaatctt
     3001 aacggcgggg gcagcccggg agacgtgatc ctgccgatgc gcccggtgat cctgaaagcc
     3061 gcagccggga gtggttcgat gtttgtggcg cactgcccga aggaggataa gtgatggaaa
     3121 cggcacccgt acagtacgct attgtggatg gtgctgtgga agaaggattg ctggattttc
     3181 tgaacgaagt gaatccccca cactgctgtc tgtatgcaga acccatacag ccggatctgg
     3241 tcgcgctggc cccctatctc gtggaagttg ttccggaggt tgaagcatgg ctgagagata
     3301 aggcgtcgcc atggggaata tatctgactt ctgagtcatc catgcgtgag ctacagcaac
     3361 acttcagacg ttatctgtgg gttaggatcc ccgaacagga gaaacctgtt ctcatgcgat
     3421 tttatgatcc ccggaatata tgggtattag cggaagttct taccccccgc cagcttcttt
     3481 tttttatcaa ccccgtcaga cagctaagta cccgctatgg tgaagaatgt cgtgaggata
     3541 atttttcttc cgtccgtccc gcagaaacta tgaatataag ggcagaacgg ccatcacaac
     3601 tgatgctgag ttacagacaa tatagccaac tggaacgaaa ggcacgggat aattatcttg
     3661 atacattatc tgtatttatc gaagaaaatg cagaaaaaga agggtgggat gattcatcaa
     3721 aagctgaatc atcccgaatt ctggcagaag attatttctc tttttgccag tcattaaata
     3781 tagcagatga tcgttccgtc agaacaatga cgcttatctt actgaaaaaa aacataatcg
     3841 atctccgata tattccggat gactggtatg aactattcag caatcagtca tatccgggac
     3901 atattcgggt acatgaactt gcacaacagg aactgggatt tatcccgcaa taaaaagaca
     3961 aacggataga ttatatgaca tctccatctt cgacagccgg aaagccttgt gtggaatgcg
     4021 gcatacctga tccatgtatt tttgatgtta ttagtgattt tccagaggcc ggcgagaaac
     4081 atttctggtc aaaagaagga atggcgcgtt ttaagctact ggacgatggt tttggatgtg
     4141 atggtataat taccattacc taccagtgcg acaacagtgg gacacatgag gcatggttag
     4201 aggaagaatc agaaaacagt cgtaagatac tgcaaccttt tggaaaaccc aataatgtta
     4261 cattatatta tgaaggtttc aagggtggaa tgaatatcat tgatgcgttt cattctccgt
     4321 gggagtatct gagttcaatt accatgccac atgatgttct ttatgaacca gcacattaca
     4381 cagtcagaat tgatagctgt tacgagcaca actcctatgt cgagattaag gtttatcctt
     4441 cattagaaat tcggtttacc acagggctta gttatgagat ttcgtcatca caacagacac
     4501 gagcggtaaa agatcgacgt gatgaacgga gaaaagctcg ggaggcgatg gaagatacaa
     4561 aacctaaaga taaaaataaa ctacgaagcg gatggacaaa tacaaccgct gagtttgagt
     4621 tgtttcacaa gacatctctt accgttgaac tgggattaaa agtcgctaat gttgaatata
     4681 cacacaagta tgtagaggaa ataaaaaaac tcagacaaat aaaacaactg gagcagatga
     4741 gtagaataga tgcgctgatt aaaagcatta acaaatactt tgcgccagat cctgacaaca
     4801 aagatggtac gcgtaaatat gcgttattca gtgcaaaaat agaacctgtc acactgggga
     4861 tttcttatgc ttatcaatac actgatatta aagatggtcc gtgccatttt tatggtctat
     4921 atggtgctcc atttttcaaa gcatcattcc gatttgatat catctcattt atatgtgcat
     4981 actgcaaaat ggaagatctg gtaaaccgat gccgtgaata tctccgcaag catggaacca
     5041 gtgttgaatg ctatatcgaa atttcaccgg aagttaatct cgatcttggg gctgtatatt
     5101 ctcaaaaaga taaagcatgg gaatttaaca ctaaagaaag caatattaaa cttggtttga
     5161 aaggtgaggt tagtgcaaca tttgagacga atgtttttgt cgtgaaattg aaatctgagg
     5221 ttggtgcttc tatagaagca aaagctgggt ttggatttga taaacgagat gatggaatgg
     5281 atcttgttct atttcatgat ggagtgacgg gcgcgtttaa agttaaggtt gacatatctc
     5341 gcggtgataa agatagaaaa gagaatagtg agaaacccga aaaagaaaat gaacttaagt
     5401 ggcaactatg taaacctttg gaagtagatg actcaccatt acggattaat ttgtatggta
     5461 aggaaaatac tgtggaaaaa aaagtaacgc cagtgacgca gtggcagata ggcactgatt
     5521 attgaacggc gaagaatgaa tcaaattaaa gataataagg taattatgaa aataaaaaat
     5581 ttaatatcag tcattttact atcaggaggt attatgggga ctggattgta ctcgagcgat
     5641 aaccatcaaa aaatccgcag caggtttaat atacaggaat catattgtgc cattaagact
     5701 aatggtgtcc ttggattcag caaccgaaag gatgtattgc gagaaaatgg tgattcaacc
     5761 ggaaccacca gttccagcac taatgccatg atgctgatgg aaaatggtga aaatgaaatc
     5821 agtctggaga ttggggcgtt aaggtggttt tctgataaac ctgccagtac cgaagaacga
     5881 gggcatttct cccaaaaagc agggtgcagt ctggatttgg ttcgttttgt taagcaggaa
     5941 gaaaccatac tttcttcgat aaaggtgacc atcaaccagc agggaatacc tgaagcgcag
     6001 ccagacagca tgcatcctgt tatccgaaaa gagattctgg ctgagcaggc agaacccgga
     6061 tttattgatc cagactattt taatgaaact tatttcccga aagggatgaa ggtgtatcaa
     6121 tttacacaaa aggtctcggt ggcggggctt cctgaatggg cctggacgcg agctacgccc
     6181 tttaccggaa gcgatgaaca gttacagaag ctaaaagcag cctatatcga aatggctgca
     6241 attattagca gcggggacag ggcacgtctg aaagcttctc acaaagaggc actgaaagcc
     6301 tggtcagtca caaccggtga cagtgaggat gaaatattgc tctcgctcta tccaaaagaa
     6361 gaaatagaag gcaggaaagc cagaatgcag cccatcagat ggggcgatta cgctatccgg
     6421 gttatgaata atggacgtat ggtgcagcta tataataaat cgaaaccaac atattctccg
     6481 ctgacttatc gtttcataga tgaagatggt gatgaagtta tggggtatta cgctccggta
     6541 ttttcgctga ttgatggtca gtttatccct gtcacttgat taacctgttg ggctacaaat
     6601 caggatgtcc ggtaaggtga ttattgctga gttaagcacc ttaccatttt tttaattttt
     6661 tgtcgctgat attccagaca ggaaaacaat atgcctttag ctgccagatt aaacgacaaa
     6721 ggtacccagc atgatggcta ccgtgagaca gtcatcattg cggcttcatc gacggtattt
     6781 attgatggat tacctgccgc ccgccaggga gatccgctga cgccgcatga taaaccgaag
     6841 catccccctc atccccgaaa aatcgccaga ggctcgtcaa ccgtctttat tgacggcctg
     6901 cccgccgcca gaaccggtga tgctattgac tgcggcggcg tggttattgg tggcggtacg
     6961 gtaaatatag gctgatatgt ctgagaggta tttatgtcct taaaaggtct tcgctttacg
     7021 ctggaggttg acggccagga gccggacacc tttgcagtgg tgaatttcag gttaatccag
     7081 aaccagtctt atccgtttgt gatgagtgtg gatgtcgcca gcgattcttt tatgcagacg
     7141 gcggagatgc tgctggagaa gaacgcaacg ctgactatct ggcagggtgt catcccgctg
     7201 cgttatgtca caggcgtggt ggcggggttt ggtatgcagg agaacaacgg ctggcagatg
     7261 cgttatcacc tgcgtattga gccgccgtta tggcggtgcg ggctgcggcg gaacttccgt
     7321 atcttccagc agcaggatat ccggacgata tcggccatgc tgctgaacga gaacggcgtc
     7381 acagagtgga cgccgctgtt ttatgaggac catccggcgc gggagttctg tgtccagtac
     7441 ggggaaagcg acctggcgtt tctggcgcgg ctgtgggcgg aggaggggat tttcttcttt
     7501 gagcggtttg cggcggacag tccggagcag aagctgacgc tgtgcgacga tgtggcgggg
     7561 ctgtcacagg cgggggag
//
LOCUS       DAZFRE010000170         7493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFRE010000170 DAZFRE010000000
VERSION     DAZFRE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.171"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            252..1199
                     /gene="cyoA"
                     /locus_tag="ACHKUI_004773"
     CDS             252..1199
                     /gene="cyoA"
                     /locus_tag="ACHKUI_004773"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6600800.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1221..3212
                     /gene="cyoB"
                     /locus_tag="ACHKUI_004774"
     CDS             1221..3212
                     /gene="cyoB"
                     /locus_tag="ACHKUI_004774"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6600801.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3202..3816
                     /locus_tag="ACHKUI_004775"
     CDS             3202..3816
                     /locus_tag="ACHKUI_004775"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6600802.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3816..4145
                     /locus_tag="ACHKUI_004776"
     CDS             3816..4145
                     /locus_tag="ACHKUI_004776"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6600803.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4157..5047
                     /gene="cyoE"
                     /locus_tag="ACHKUI_004777"
     CDS             4157..5047
                     /gene="cyoE"
                     /locus_tag="ACHKUI_004777"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6600804.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5196..6566
                     /locus_tag="ACHKUI_004778"
     CDS             5196..6566
                     /locus_tag="ACHKUI_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600805.1"
                     /translation="MNDYKMTSGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPDNVMADEKLEKSLLTTPGVKDVLLAKLEHSVYVKIDSKITNRFEIERV
                     INEVSY"
     gene            6739..>7493
                     /locus_tag="ACHKUI_004779"
     CDS             6739..>7493
                     /locus_tag="ACHKUI_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFO6600806.1"
                     /translation="MVKPLLTIITLLYCASSSAKSGSPPASTITFIAQIEHVELNKTA
                     LSHDMKLILADRFYFKTSNPCETNSISARPESMGITDKAYADKLRNLETPFLDDRYFF
                     MTINQCDGNGSPMLTDIKVCTTELCGAEYTKKIPTVWLDETLKPTAKRLAIYFIQSPL
                     PYDDKKKQWKVTGWYISSTDNPSSTKQIAFEGYTDTDNFSNSKFISSFQSWFENGKIK
                     SIVNYNPQGERDGKYESYYNSNGNVYESLSFSNG"
BASE COUNT         1730 a         1807 c         1887 g         2069 t
ORIGIN      
        1 tgttaaataa ttgttttatt ttatattgat tataccaatt gcccacccaa actccttctg
       61 cactcccctt tcgttataac gcccttttgc aacagcttct taaaatcaac ttgatatgtt
      121 ttgcaacata tgtgacctga cagccaaatc caagtaacag gaatttaatc atgtttacag
      181 taatgtaacc ttcccgtaaa atgcccacac actttaaacg ccaccagatc ccgtggaatt
      241 gaggtcgtta aatgagactc aggaaataca ataaaagttt gggatggttg tcattatttg
      301 caggcactgt attgctcagt ggctgtaatt ctgcgctgtt agatcccaaa ggacagattg
      361 gtctggagca acgttcactg atactgacgg catttggcct gatgttgatt gtcgttattc
      421 ccgcaatctt gatggctgtt ggtttcgcct ggaagtaccg tgcgagcaat aaagatgcta
      481 agtacagccc gaactggtca cactccaata aagtggaagc tgtggtctgg acggtaccta
      541 tcttaatcat catcttcctt gcggtactga cctggaaaac cactcacgct cttgagccta
      601 gcaagccgct ggcacacgac gagaagccca ttaccatcga agtggtttcc atggactgga
      661 aatggttctt catctacccg gaacagggca ttgcaaccgt gaatgaaatc gctttcccgg
      721 cgaacactcc ggtgtacttc aaagtgacct ccaactccgt gatgaactcc ttcttcattc
      781 cgcgtctggg tagccagatt tatgccatgg ccggtatgca gactcgcctg catctgatcg
      841 ccaacgaacc cggtacttat gacggtatct ccgccagcta cagcggcccg ggcttctcag
      901 gcatgaagtt caaagctatt gcaacaccgg atcgcgccgc attcgaccaa tgggtcgcaa
      961 aagcgaaaca gtcgccgaac agcatgtctg acatggcagc gttcgaaaaa ctggccgcgc
     1021 ctagcgaata caaccaggtg gaatatttct ccaacgtgaa accagacttg tttgccgatg
     1081 tgattaacaa gtttatggct cacggtaaga gcatggacat gacccagcca gaaggtgagc
     1141 acagcgcaca tgaaggtatg gaaggcatgg acatgagcca cgcggaatcc gcccattaaa
     1201 ggggttgagg aagaataaag atgttcggaa aattatcact tgatgcagtc ccgttccatg
     1261 aacctatcgt catggttacg atcgctggca ttattttggg aggtctggcg ctcgttggcc
     1321 tgatcactta cttcggtaag tggacctacc tgtggaaaga gtggctgacc tccgttgacc
     1381 ataaacgtct cggtatcatg tatatcatcg tggcgattgt gatgttgctg cgtggttttg
     1441 ctgacgccat tatgatgcgt agccagcagg ctcttgcctc ggcgggcgaa gcgggcttcc
     1501 tgccacctca ccactacgat cagatcttca ccgcgcacgg cgtgattatg atcttcttcg
     1561 tggcgatgcc tttcgttatc ggtctgatga acctggtggt tccgctgcag atcggcgcgc
     1621 gtgacgttgc gttcccgttc ctcaacaact taagcttctg gtttaccgtt gttggtgtga
     1681 ttctggttaa cgtttctctc ggcgtgggcg aatttgcgca gaccggctgg ctggcctatc
     1741 caccgctatc gggaatagag tacagtccgg gagtcggtgt cgattactgg atatggagtc
     1801 tccagctatc cggtataggt acgacgctta ccggtatcaa cttcttcgtt accattctaa
     1861 agatgcgcgc accgggcatg accatgttca agatgccagt atttacctgg gcatcactgt
     1921 gcgcaaacgt actgattatt gcttccttcc caattctgac ggttaccgtc gcgttgttga
     1981 ccctggatcg ctatctgggc acccatttct ttaccaacga tatgggtggc aacatgatga
     2041 tgtatatcaa cctgatttgg gcctggggcc acccggaagt ttacatcttg atcctgcctg
     2101 tattcggtgt gttctccgaa attgcggcaa ccttctcgcg taaacgtctg tttggttata
     2161 cctcgctggt atgggcaacc gtctgtatca ccgtgctgtc gttcatcgtc tggctgcacc
     2221 acttctttac gatgggtgcg ggcgcgaacg taaacgcctt ctttggtatc accacgatga
     2281 ttatcgccat ccctaccggg gtgaagatct tcaactggct gttcaccatg tatcagggcc
     2341 gcatcgtgtt ccattctgcg atgctgtgga ccatcggttt tatcgtcacc ttctcggtag
     2401 gcggtatgac tggcgtgctg ctggcagtac cgggcgcgga cttcgttctg cataacagcc
     2461 tgttcctgat tgcgcacttc cataacgtga tcatcggcgg cgtggtcttc ggctgcttcg
     2521 cagggatgac ctactggtgg cctaaagcgt tcggtttcaa actgaacgaa acctggggta
     2581 aacgcgcgtt ctggttctgg atcatcggct tcttcgttgc ctttatgcca ctgtatgcgc
     2641 tgggcttcat gggcatgacc cgtcgtttga gccagcagat tgacccgcag ttccacacca
     2701 tgctgatgat tgcagccagc ggtgcggtac tgattgcgct gggtattctc tgcctcgtta
     2761 ttcagatgta cgtttctatt cgtgaccgcg accagaaccg tgacctgact ggcgacccgt
     2821 ggggtggccg tacgctggag tgggcaacct cttccccgcc tccgttctat aactttgccg
     2881 ttgtgccgca cgttcacgaa cgtgatgcat tctgggaaat gaaagagaaa ggcgaagcgt
     2941 acaaaaagcc tgaccactat gaagaaattc atatgccaaa aaacagcggt gccggtatcg
     3001 tcattgcggc tttctccacc atcttcggtt tcgccatgat ctggcatatc tggtggctgg
     3061 cgattgttgg cttcgcaggc atgatcatca cctggatcgt gaaaagcttc gacgaggacg
     3121 tggattacta cgtgccggtg gcagaaatcg aaaaactgga aaaccagcat ttcgatgaga
     3181 ttactaaggc agggctgaaa aatggcaact gatactttga cgcacgcgac tgcccacgcg
     3241 cacgaacacg ggcaccacga tgcaggcgga accaaaatct tcggattttg gatctacctg
     3301 atgagcgact gcattctgtt ctctatcttg tttgctacct atgccgttct ggtgaacggc
     3361 accgcaggtg ggccgacagg taaggacatt ttcgaactgc cgttcgttct ggttgaaact
     3421 ttcttgctgt tgttcagctc catcacctac ggcatggcgg ctatcgccat gtacaaaaac
     3481 aacaaaagcc aggtgatctc ctggctggcg ttgacatggt tgtttggtgc cggatttatc
     3541 gggatggaaa tctatgaatt ccatcacctg attgttaacg gcatgggtcc ggatcgcagc
     3601 ggcttcctgt cagcattctt tgcgctggtc ggcacgcacg gtctgcacgt cacttccggt
     3661 cttatctgga tggcggtgct gatggtgcaa atcgcccgtc gcggcctgac cagcactaac
     3721 cgtacccgca tcatgtgcct gagcctgttc tggcacttcc tggatgtggt ttggatctgt
     3781 gtgttcactg ttgtttatct gatgggggcg atgtaatgag tcattctaac gcaagcagcg
     3841 gcgcatccca tggcagcgta aagacctaca tgacaggctt tatcctgtcg ataatcctga
     3901 cggtaattcc gttctggatg gtgatgacag gggctgcctc tccggccgta attctgggaa
     3961 caatcctggc aatggcagtg gtacagattc tggtgcatct ggtgtgcttc ctgcacatga
     4021 ataccaaatc agatgaaggc tggaatatga cggcgtttgt cttcaccgtg ctaatcatcg
     4081 ctatcctggt tgtaggctcc atctggatta tgtggaacct caactacaac atgatgatgc
     4141 actaagagcg gcggttatga tgtttaagca atacctgcaa gtaacgaaac caggcatcat
     4201 ctttggcaac ctgatctcgg tgattggggg attcctgttg gcctcaaagg gcagcattga
     4261 ttatcccctg tttatctaca cgctggttgg ggtgtcactg gttgtggcgt cgggttgtgt
     4321 gtttaacaac tacatcgaca gggatatcga cagaaagatg gaaaggacga agaatcgggt
     4381 gctggttaaa ggcctgatct ctcctgctgt ctcgctggtg tacgccacct tgctgggtat
     4441 tgctggcttt atgctgctgt ggtttggcgc gaatccgctg gcctgctggc tgggggtgat
     4501 gggctttgtg gtttatgtcg gcgtttatag cctgtacatg aaacgtcatt ctgtctacgg
     4561 caccttgatt ggttcgctct ccggcgctgc gcctccggtg atcggttact gtgcggtaac
     4621 cggcgagttc gatagcggcg cagcgatcct gctggctatc ttcagcctgt ggcagatgcc
     4681 tcactcttat gccatagcca ttttccgctt taaggattat caggcggcaa acattccggt
     4741 actgccggtg gtgaaaggca tttcggtggc gaagaatcac atcacgctgt atatcatcgc
     4801 ctttgccgtt gccacgctga tgctctctct tggcggctac gctgggtata aatatctggt
     4861 ggtcgccgct gcggtgagcg tctggtggtt aggcatggct ctgcgcggct ataaagttgc
     4921 tgatgacaga atctgggcac gcaagctgtt cggtttctct atcatcgcca tcactgccct
     4981 ctcggtgatg atgtccgttg attttatggt gccggactcg catacgctgc tggctgctgt
     5041 gtggtaacac aacctctcag ttaaaaaggt gctacggcac cttttttctt agcattagaa
     5101 tcatatccct ctcgaaatat ttactaaaaa atccgcatgt ttaccccatt cgtttgccgc
     5161 tttacactag tcgcgaattt aaaacagagg tggtaatgaa cgattataaa atgacgtcag
     5221 gtgagcggcg cgcgacctgg ggtttaggca ccgttttctc gttgcgcatg ctgggcatgt
     5281 tcatggttct gccggttctg accacgtacg gcatggctct gcaaggtgcc agcgaagcat
     5341 taatcggtat tgccattggt atttatggtc tgactcaggc cgtttttcag attccgtttg
     5401 gcctgctttc agaccgcatt ggtcgcaaac cattaattgt cggtgggctg gcggtgtttg
     5461 ccgccggtag cgttatcgct gcgctctctg actccatctg gggaattatt ctgggccggg
     5521 cgctacaagg ctccggtgcg attgccgctg ccgttatggc gctgctttcc gatctcacgc
     5581 gcgaacaaaa ccgcaccaaa gcgatggcgt ttatcggcgt gagctttggc attacctttg
     5641 ccattgcgat ggtgcttggc ccgatcatca ctcacaaact tgggctgcac gcgctgttct
     5701 ggatgatcgc tattctggca acaaccggca ttgcgttgac catttgggtt gtgcccaaca
     5761 gtagcactca cgtacttaat cgtgagtccg gaatggtgaa aggcagtttc agtaaagtgc
     5821 tggcggaacc gcggctgctg aaactcaact ttggcattat gtgtctgcat attttgctga
     5881 tgtcgacctt tgttgccctg cccggacaga tggctgatgc ggggttcccg gcggctgaac
     5941 actggaaggt ctacctggca acgatgttaa tcgcctttgg ctcggtcgtg cctttcatta
     6001 tctacgctga agttaagcgc aaaatgaagc aagtctttgt cttctgcgtc gggttgatcg
     6061 tggttgcgga aattgtgttg tggaacgcac aaacgcagtt ctggcaactg gtggtcggcg
     6121 tgcagctttt ctttgtggcg tttaatttga tggaagccct tctgccttca cttatcagta
     6181 aagagtcgcc agcaggttac aaaggtacgg cgatgggtgt ttactccacc agccagtttc
     6241 ttggcgtggc gattggcggt tcgctgggtg gctggattga cggcatgttt gacggtcagg
     6301 gcgtatttct cgctggcgca atgctggccg cagtgtggct ggcagtcgcc agtaccatga
     6361 aagaaccgcc gtatgtcagc agcttgcgca ttgaaatccc ggacaacgtg atggcagatg
     6421 aaaaactgga aaaaagctta ctgacaacgc ctggtgtcaa agatgtactg ctggcgaagt
     6481 tagagcatag cgtctatgtg aaaatagaca gtaaaatcac caatcgcttc gaaatagagc
     6541 gagttatcaa cgaagtatct tattaaatat taagcacggt gctattcatg aacagcaccg
     6601 tgcttcgtca cgtcattatt ctttaaatcc cacattatta aaacttcatg taattccata
     6661 aaactggacg attcgatcag aagtaaatta tactttttcc gaaaatcagg gataaattat
     6721 tcatatggag aatatattat ggtaaaacca ttacttacta ttatcactct attgtattgc
     6781 gccagttcat cagcaaaaag tggatcccct cccgcaagca ctataacttt catcgcacaa
     6841 atcgagcatg ttgagctaaa taaaacagca ttatctcatg atatgaaact cattcttgcc
     6901 gatcgttttt atttcaagac cagtaatcct tgtgaaacaa attcaatttc cgctcgacca
     6961 gaatcaatgg gcattactga caaagcctat gccgacaaac taagaaattt agaaactcct
     7021 tttcttgatg atcgctattt ctttatgacc atcaatcagt gtgacggtaa tggtagcccc
     7081 atgctgacag atataaaagt atgtacaaca gaattatgtg gtgctgaata tacaaagaaa
     7141 atacctactg tatggcttga tgaaacatta aaaccaactg ctaaacgcct agcaatttat
     7201 tttattcaat ctcctttacc ctatgacgat aagaaaaaac aatggaaagt taccggatgg
     7261 tatatctcat caactgataa tccgtcttca actaaacaga ttgcatttga aggatataca
     7321 gacacagata acttctccaa ttctaaattt atttcttcat ttcaatcctg gtttgaaaat
     7381 ggaaagataa aaagcattgt taactataat ccacaaggag aaagagatgg aaaatacgaa
     7441 agttattaca atagcaatgg taatgtctat gaatctctaa gtttttctaa tgg
//
LOCUS       DAZFRE010000171         7394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFRE010000171 DAZFRE010000000
VERSION     DAZFRE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.172"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            196..1563
                     /gene="dgcT"
                     /locus_tag="ACHKUI_004780"
     CDS             196..1563
                     /gene="dgcT"
                     /locus_tag="ACHKUI_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6600807.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIVLFFIDTVSFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVTLCITLFFVLG
                     GPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            complement(1668..1755)
                     /locus_tag="ACHKUI_004781"
     tRNA            complement(1668..1755)
                     /locus_tag="ACHKUI_004781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1719..1721),aa:Ser,seq:gga)
     gene            1989..2927
                     /gene="ghrA"
                     /locus_tag="ACHKUI_004782"
     CDS             1989..2927
                     /gene="ghrA"
                     /locus_tag="ACHKUI_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6600808.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2982..3719
                     /gene="ycdX"
                     /locus_tag="ACHKUI_004783"
     CDS             2982..3719
                     /gene="ycdX"
                     /locus_tag="ACHKUI_004783"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6600809.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3743..4297
                     /gene="ycdY"
                     /locus_tag="ACHKUI_004784"
     CDS             3743..4297
                     /gene="ycdY"
                     /locus_tag="ACHKUI_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6600810.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4399..4890
                     /gene="ycdZ"
                     /locus_tag="ACHKUI_004785"
     CDS             4399..4890
                     /gene="ycdZ"
                     /locus_tag="ACHKUI_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6600811.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(4954..5787)
                     /gene="csgG"
                     /locus_tag="ACHKUI_004786"
     CDS             complement(4954..5787)
                     /gene="csgG"
                     /locus_tag="ACHKUI_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6600812.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5814..6230)
                     /gene="csgF"
                     /locus_tag="ACHKUI_004787"
     CDS             complement(5814..6230)
                     /gene="csgF"
                     /locus_tag="ACHKUI_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6600813.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     EIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6255..6644)
                     /gene="csgE"
                     /locus_tag="ACHKUI_004788"
     CDS             complement(6255..6644)
                     /gene="csgE"
                     /locus_tag="ACHKUI_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6600814.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6649..7299)
                     /gene="csgD"
                     /locus_tag="ACHKUI_004789"
     CDS             complement(6649..7299)
                     /gene="csgD"
                     /locus_tag="ACHKUI_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6600815.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECCFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1906 a         1601 c         1781 g         2106 t
ORIGIN      
        1 cttataacca tttatatgta tttgcaagcc tgtcttgtcg cgaagaatgt ataaagcact
       61 aaggtaataa taattgcggt ctgcaactat catcattggt attgtttgtg gtggattttt
      121 atattgacaa cagtacagtt cttatatcta ttaatgacag aaagggatct acaacctaca
      181 gattggtgta gctttatgga aaaagactat ttgggaatta gtagtactgt attagtgagt
      241 ttattgtttg ggcttgcgtt ggttttggtg aatagttggt tcaatcagcc aaccgttgag
      301 gaagttgttc cacgatcaac ctatctgatg gttatgattg ttttattttt tatcgatact
      361 gtttcattta tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt
      421 atacttagtt tggctttttt aagttgtttg atttacttta ttaaaaccgt cattatcatt
      481 cagcaaatta ttgaggggcg tttgacaagc agtgttgttc aaaatgacat tgcaatttat
      541 tatttgtttc gtcagatgag tttgtgcgta ttaatatttc tggcattggt aaataaagtt
      601 agtgaaaaca caaaacagcg aaatttgttt tccaaaaaag tgactttgtg cataactttg
      661 ttttttgttc ttggggggcc gatagttgct cacatatttt ctagccatta tgagagctac
      721 gatttacaca ttgccgagtt aaccaatgaa aatggtcagg tggtttggaa agcctcatat
      781 gttaccataa tgattttcat gtggttaacc ttgctttcgg ttaatctata ctttaatggt
      841 ttacgctgtg atatctggaa cggtgtgtcg gtgattgctt tttgtgccgt gctgtacaat
      901 atctctctgt tatttatgag caggtatagc gtgtccattt ggtatattag tcgcactatt
      961 gaagtagtca gtaagttaac cgttttggta atttttatgt gccatatatt cagtgcgcta
     1021 cgagtaacaa aggacattgc acatcgcgat cccttaacca atatatttaa cagaaattat
     1081 ttttttaatg aactgacggt tcaatcagca tcggccaaaa aaacacctta ttgcgtcatg
     1141 attatggata ttgaccactt caaaaaagtc aacgacacct ggggacatcc ggttggcgat
     1201 caagtgataa agacagtggt gaatatcatt gggaaaagca tacgaccaga tgacctttta
     1261 gcgcgcgttg gcggcgaaga gtttggcgtc ttgctgacgg agatcgatac tgaatgtgcg
     1321 aaagctttag cggaaaggat tcgggaaaat gttgaacgtt taactggcga taatcctgaa
     1381 tacgccatac cacaaaaagt gacgattagt attggcgctg ttgtaacgca gaaaaatgag
     1441 ttaaacccta aggagatcta tcaactggct gataacgcac tttacgaggc gaaagagacc
     1501 gggcgtaata aggtggtagt aaaagaggca gaaattctta tcaaccgcaa ggatgatgaa
     1561 tgagtctgtc gccagttgtc gaaccccgat cggggcttct catccccccg gtgtgtgcaa
     1621 tatacgaaaa aaaagcccgt actttcgtac gagctcttct ttaaatatgg cggtgagggg
     1681 gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca
     1741 ctcggacacc tcaccaaatt gtcgttcctg tcttgctgga acgggcgcta atttagggaa
     1801 atcatgacct gaggtcaaca aactttttta aaaaatcgct cgattattca aacttcaatc
     1861 aatatgcagt tttaataagc gaaatttgct ttttttgcca ccgaccacgg atttgttatg
     1921 ctggtggctt ttgtagatca taacgataag tgcgaataaa tttcgcgcaa cgcttttcgg
     1981 gagtcagtat ggatatcatc ttttatcacc caacgttcga tacccaatgg tggattgagg
     2041 cactgcgcaa agctattcct caggcaagag tcagagcatg gaaaagcgga gataatgact
     2101 ctgctgatta tgctttagtc tggcatcctc cagttgaaat gctggctggg cgcgatctta
     2161 aagcggtgtt tgcacttggg gcaggtgttg attctatttt gagcaagcta caggctcacc
     2221 ctgaaatgct gaagccttct gttccacttt ttcgcctgga agataccggt atgggcgagc
     2281 aaatgcagga atatgctgtc agtcaggtgc tgcattggtt tcgacgtttt gacgattacc
     2341 gcatccagca aaatagttcg cattggcaac cgctgcctga atatcatcgg gaagatttta
     2401 ccatcggcat tttgggtgca ggcgtactgg gcagtaaagt tgcacagagt ctgcaaacct
     2461 ggcgcttccc gctgcgttgc tggagtcgaa cccgtaaatc gtggcctggc gtgcaaagct
     2521 ttgccggacg ggaagaactc tctgcatttc tgagccaatg tcgggtattg attaatttgt
     2581 taccgaatac acctgaaacc gtcggcatta ttaatcaaca attactcgaa aaattaccgg
     2641 atggcgcgta tctcctcaac ctggcgcgtg gtgttcatgt cgtggaagat gacctgctcg
     2701 cggcgctgga tagcggcaaa gttaaaggcg caatgctgga tgtttttaat cgtgaaccct
     2761 taccgccgga aagtccgctc tggcaacatc cacgcgtgac gataacacca catgtcgccg
     2821 cgattacccg tcccgctgaa gctgtggatt acatttcgcg caccattgcc cagctcgaaa
     2881 aaggggagag ggtctgcggg caagtcgacc gcgcacgcgg ctactaataa agcatctggt
     2941 ttcctgctat ccttggcggg aattgaatac aggagagagt tatgtatccc gtcgaccttc
     3001 atatgcatac cgttgccagc acgcatgcat atagcacatt gagtgattac attgcccagg
     3061 ccaaacaaaa gggcattaaa ctttttgcga tcaccgatca tggcccggat atggaagatg
     3121 cgccgcatca ctggcacttc attaacatgc gtatctggcc gcgagtggtt gatggggtag
     3181 ggatcctgcg cggcatcgaa gctaacatta aaaatgttga tggtgaaatt gattgcagcg
     3241 gcaaaatgtt tgattcgctg gatctgatta ttgccggttt tcatgagccg gtttttgcgc
     3301 cacatgacaa agcgaccaat acacaagcga tgatcgccac tatcgccagc ggcaatgtgc
     3361 atataataag ccatcccgga aacccgaaat atgaaataga tgttaaagcc gttgctgaag
     3421 cagccgcgaa acatcaggtg gcgctggaaa taaataattc ctctttttta cactcacgta
     3481 agggaagtga agacaattgt cgtgcggtag ccgcagcggt acgtgacgct ggaggttggg
     3541 tggcattagg ctcggattct cacaccgcgt ttaccatggg tgaatttgaa gagtgtctta
     3601 aaatcctcga cgcggtagat tttccgccag agcgcatttt gaatgtttct ccgcgccgct
     3661 tacttaactt ccttgaatct cgcggtatgg caccgattgc ggaatttgca gacctttaat
     3721 tactagtaat ggaaatatat taatgaacga gttttctatc ctctgtcgtg tgctgggttc
     3781 gctctattac cgccaaccac aagatccttt actggtgccg ctgtttaccc tgattcgtga
     3841 ggggaaactg gctgcgaact ggccactgga gcaggatgag ttgctgacac gtttgcagaa
     3901 aagctgtgat atgacgcaag tctctgccga ttacaatgcg ttgtttatcg gcgatgaatg
     3961 tgctgtgccg ccatatcgta gcgcatgggt tgagggcgca acggaagcgg aagtgcgcgc
     4021 ttttctttcc gaacgaggga tgccactagc ggatacgcca gccgatcaca tcggcacatt
     4081 gctgcttgcg gcttcctggc tggaagatca gtcaacggaa gatgagagcg aagcactgga
     4141 aacactgttc agtgagtatc tgttaccctg gtgtggtgcg ttccttggca aagtggaggc
     4201 ccatgcaact acgcctttct ggcgcaccat ggcaccgcta acccgcgatg ctattagtgc
     4261 aatgtgggac gagctggaag aagattctga agagtaaatg tgatctgtat cactcataac
     4321 tgcaacgagt aatttgttgt tgcataaaat gtgtgctcga tctcattcat ggccgcgttt
     4381 tctgctatca tgcgcggcat gaacatactt ctctctattg caatcacaac aggcattctc
     4441 tccggtatct ggggatgggt ggctgtttcc ctgggcttac taagctgggc gggcttcctg
     4501 ggctgtacgg cctactttgc ctgcccgcaa ggtgggctga aagggctggc gatctccgct
     4561 gcaaccctgc ttagtggcgt ggtgtgggcg atggtcatta tttacggtag tgcattggca
     4621 ccacatctgg aaatactcgg ttatgtaata accggtattg tcgcgtttct gatgtgtatc
     4681 caggcgaaac agctgctgct ttcatttgtt ccggggacgt ttataggcgc atgcgcgacg
     4741 tttgccgggc agggtgactg gaaactggtg ttaccttcgc tggcgcttgg actggtattt
     4801 ggttacgcaa tgaaaaacag cggcctgtgg ttggcggcgc gtagtgcaaa gacagctcac
     4861 cgtgagcagc aaatcaaaaa taaagcgtga ggggcactca cgctttcgct taaaccgtaa
     4921 aatgccggat gataattccg gcttttttat ctatcaggat tccggtggaa ccgacatatg
     4981 gcggtatttc accagaatgt cattctgccg ttctgcttta ttttgcaaat cccacagacc
     5041 acggtcgata ccatcattaa tcaggaaaat gacccctgtt tcgatagccg acatcaggca
     5101 cagcataaca ggttcgttcg aggtgtaacc cacttcccct tccagcaagc gctggtagtc
     5161 aataaagcgg aaaaccccgg cctgaacttc ataagaaagt atcgtcttac tggtgttcac
     5221 cgaggagagg atctcaccgg tactcacatt gacgacgcgc aggttcacgg caatctgatc
     5281 gagctggtat tgcgtgtcgg caccgattcc aaaatatctt gccccaaccc cgcccgattt
     5341 gacgttgctt tcataaccga taatcgaacc ttcaaccatg atatttgccg ccgttaaaga
     5401 ttgcagcggg attcggttat taatggcaac tgtgccgttt tcttgtgccg cacgaataat
     5461 cttgcgctcg ttaagcagat tttgtaagcc ctggcgctcc agcggtataa accagcgaga
     5521 atctttcaat gccgtgacca gcattgccgt ggcgctttgc ggaacagcag tggagaagtt
     5581 gcttgccggg tagggtttaa attgcccggt ttcgtcctga atgttgtata ccgaaacaaa
     5641 gatcttaccc gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat
     5701 taatgtcggt ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac
     5761 ggcaaccaaa agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa
     5821 tcggttgagt tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga
     5881 tctgtcacgt tcaactgcaa ttgaccatcg cggttagcaa tctcaacaat ataatcgttg
     5941 gtcaccatgc ggcccggttt accggtatta atattcgaca gtagcccacc caaaatttgt
     6001 gactggatgg cctgagtaaa gttatctaac gccgagggcg tttcaatacc aaagtcatcg
     6061 ttatagctcg gatctttata agagttttgg gcctgagcgc tatttaacaa aaaagcgcca
     6121 ttatttgggt taccaccaaa gtttggatta cggaactgga aagtcatggt tccagcccaa
     6181 cttaatggcg aaataagcat gagtagaact actgcatgtt tgacacgcat tgcagcctcc
     6241 gaacaattta ttatttaaaa ttcatcatgc gccaaatcgc ccgtacttaa taacgcctgg
     6301 tttatctggc gacgatttag tgcttcttca gtttgaatca gtgcaaagac gacagttttc
     6361 tcgaagtctc ttttcaacgg aaataaaaaa gtctggaaaa taacgtcctg attcaccgtt
     6421 attgtgatcc agcttcccca tcgtgcactg ggcctttcat taatcgttaa gttacccgta
     6481 tagtcacttt cccatttatc actaaaggct cggtaaaaat catggccaat agatgaaaca
     6541 gtatggtcag ttaacaatcc cgggacttct acctcaacgg cgtgaagatt ccctgcggcg
     6601 aacagaaatt ctgccgccac aatccagcgt aaataacgtt tcatggcttt atcgcctgag
     6661 gttatcgttt gcccaggaaa ccgcttgtgt ccggtttttt acggctatct tcttgaaaag
     6721 attataaaga tgcgttttaa ccgtattttc gctgatgaac aacgaacgag cgatctcgtt
     6781 attagacgcg ccgatacgca gcttattcag gatctctttt tcccgatgag taaggagggc
     6841 tgattccgtg ctgttataac gatagttacc tgaatgcgta atcaggtaac tggcaagctt
     6901 ttgcgtaaag cagcattcgc cgcgcaggac gccttgcaac ccattgacaa cacgttcttg
     6961 atcctccatg gcataaaaaa cgccgttgat atgaggccag ttttcaatgt cgcggtacgg
     7021 gtaatcttca ggcgtattta gcaacaatat tttgatattg ttgtttttcc tgctcaaagt
     7081 atcctgccaa taatggataa gctttttatc cgcttccatc atatccagaa gaataataga
     7141 gcctgaagat atatcgtcca gagaacgttg aatattatgt aattttcctg taattgccag
     7201 cgattgttta aggtgctgca agagagctgt cgcctgcaaa gaaggtttag tgatcaacaa
     7261 taatgtatga ccatgaatac tatggacttc attaaacatg atgaaacccc gcttttttta
     7321 ttgatcgcac acctgacagc tgcctctaaa atagaagcac cagaagtact gacagatgtt
     7381 gcactgctgt gtgt
//
LOCUS       DAZFRE010000172         7382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFRE010000172 DAZFRE010000000
VERSION     DAZFRE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.173"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            572..799
                     /gene="daaF"
                     /locus_tag="ACHKUI_004790"
     CDS             572..799
                     /gene="daaF"
                     /locus_tag="ACHKUI_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaF"
                     /protein_id="HFO6600816.1"
                     /translation="MKINKLTLNERKNDILSYFGEINAPCRTSEVAEHLGVSAYQARH
                     YLQCLEKEGKIKRSPVRRGASTLWEISSIPP"
     gene            complement(1062..1991)
                     /locus_tag="ACHKUI_004791"
     CDS             complement(1062..1991)
                     /locus_tag="ACHKUI_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6600817.1"
                     /translation="MSRKKDKNTSTPHDAAFRAFMASPEVARDFVSLHLPEEYRQLCD
                     LSTLRLEPCTFVDQDLKQYACDALFSMKTVSGEDGYVYCLVEHQSTENAYMAFRMLRY
                     AVAAMQRHVEAHKVLPLVIPVLFYHGERSPYPYSMNWLDGFSDPDIVAKIYTRAFHLV
                     DVTVMDDDEIMHHRRLGALTLLMKHSRSRDLMLQMDRLVQLIQDSLNEELAQVLFHYL
                     LNGSEHVTVTFLQTLAQRLPQHEDNIMTLAERIEQKGIAKGIERGLAQGIEQGKLEGK
                     LETACNMLNAGMDRQTVLQMTGLSNDQLNMLKH"
     gene            2123..2647
                     /locus_tag="ACHKUI_004792"
     CDS             2123..2647
                     /locus_tag="ACHKUI_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6600818.1"
                     /translation="MGTLSIDRKPEGIYNRSHKTVQTTPQPGKTATEYKVKPAYLTRQ
                     NDNQKAADKTPWQNMTKRQRKNRKRINRLVELWPELFSREKPKPLKVGIFDELIQDIA
                     IRELAFGAGTLRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     ALNDRRKRPRSATE"
     gene            complement(2758..3291)
                     /locus_tag="ACHKUI_004793"
     CDS             complement(2758..3291)
                     /locus_tag="ACHKUI_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600819.1"
                     /translation="MQITEALLAEPGDIRRFIQQAVDHWPRLLAVHFTLHSAEGINGQ
                     QIHAFCTFFYERITERNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSICHPRASA
                     TVDEQCSQVVDLLQQTWGEISAGGQCRVERCYQVARDDTSGQYVALKTAALSFALPVV
                     SGITKHPVQSAALVSSR"
     gene            complement(3569..3802)
                     /locus_tag="ACHKUI_004794"
     CDS             complement(3569..3802)
                     /locus_tag="ACHKUI_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6600820.1"
                     /translation="MTSITGHNSVLLRADDPLIDMNYITSFTGMTDKWFYRLISEGHF
                     PEPIKPGRSSRGYRSEVEQWMQQRTEESRGGVA"
     gene            complement(3857..4059)
                     /locus_tag="ACHKUI_004795"
                     /pseudo
     CDS             complement(3857..4059)
                     /locus_tag="ACHKUI_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4253..4663)
                     /locus_tag="ACHKUI_004796"
     CDS             complement(4253..4663)
                     /locus_tag="ACHKUI_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6600821.1"
                     /translation="MNGIKTGLGITPGEHIISADAALSRKIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCDKFF
                     PGKKFCEACGTGIYCRAVCTKYVYRYRSDLLLDP"
     gene            complement(4660..5043)
                     /locus_tag="ACHKUI_004797"
     CDS             complement(4660..5043)
                     /locus_tag="ACHKUI_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6600822.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAYYTCHLCGSVLRYHPQH
                     DTERPWFEHTDDGLTEHGHECPYVQPEHREVQLIKRLQQFVPDALPVVRKACWYCRQC
                     RHAYYGEQYCTHCQTGGFSEEAVAG"
     gene            complement(5211..5777)
                     /locus_tag="ACHKUI_004798"
     CDS             complement(5211..5777)
                     /locus_tag="ACHKUI_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6600823.1"
                     /translation="MTYKYNPFWQQRIREMVLCALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLVNR
                     DTWCRAGDYRDPGSLAGMIKQAWCSALGVDTGDHTTLVHFPVRPAVWLARNDDTGFQQ
                     VLERADYLAKESTKAYGTGERNFGCSRG"
     gene            5973..6167
                     /locus_tag="ACHKUI_004799"
     CDS             5973..6167
                     /locus_tag="ACHKUI_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600824.1"
                     /translation="MVIRKKKCRECDNAITHNTVCCPYCGSVDPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            complement(6447..6570)
                     /locus_tag="ACHKUI_004800"
                     /pseudo
     CDS             complement(6447..6570)
                     /locus_tag="ACHKUI_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT         1852 a         2012 c         1831 g         1687 t
ORIGIN      
        1 cgtcacgcca tttcctgttg gatcatgtaa atgacactca ggttacaaaa ccggccagaa
       61 taatcggcgc caggcggact gagttattgt cgtctcatag gacgtaaata aaacacttgc
      121 aggatatgac atatcccgat gagtcattaa aaataataag atgaacacag cacaaatcat
      181 taaatcttct gaatatacgg gacacagaaa gggaaatccg gtacatcacc tgttttcgta
      241 ttgtatgaat acccgtggcg cgacagcgga ctttattgct gcaaaccgtc atgcttcccg
      301 gactcagagg agagtcacat cagcatgagc gaccaggacc acaccggtga gtgtttttaa
      361 gatgaaaggt atggtggcag tcacagttgc gagaaccggc atacacccgt gacgataaac
      421 aactgtgcag aatgacaaca ggagaaatga acagtaatga ggggcatcat tcgcagaacg
      481 ccggatatca tacatcatcc ccggtcagct ggacgcatca ccgccagagg aactataatc
      541 atccggaggc tgaccatctg ttaataacgg catgaaaata aacaagttaa cactgaatga
      601 acgtaagaat gacattcttt cttatttcgg agaaatcaat gccccctgca gaacctcaga
      661 agttgctgaa catttagggg taagtgctta tcaggccaga cattacctgc agtgtctgga
      721 gaaggaaggg aaaattaaac gctcaccggt acgacgggga gccagtacgc tgtgggaaat
      781 cagcagtatt ccgccataac ccgtcaggac agatgtcgtt acggacaaaa agcggttccg
      841 gaaaacattt taccactgtg aatgaccatc atccctgttc agacagcggc tgtttccgtg
      901 aaaaaaatca actggttgct ggcgatataa tgataatacc ttcacggagt gggtgaggga
      961 cagggctcac ccgtccagtg caataaaata tgtaagaatg acacaatata aataaccgcc
     1021 tccgggtgcg aatgtcccgg aagcgctccg gactaatatt gtcagtgttt cagcatattc
     1081 agctggtcat ttgaaagccc ggtcatctgc agcaccgtct gacggtccat tccggcattc
     1141 agcatattgc aggcagtttc cagcttccct tccagtttac cctgctcaat cccctgtgcc
     1201 agtcctcgct caatgccctt ggcaatacct ttctgctcaa tgcgttcagc cagtgtcata
     1261 atgttgtcct cgtgctgggg cagacgctgc gccagcgtct gcaaaaacgt caccgtcaca
     1321 tgttcactgc cattcagcag atagtgaaac agtacctgtg ccagttcttc attcagggag
     1381 tcctgtatca gctgaaccag cctgtccatc tgtaacataa ggtcacggct gcggctgtgc
     1441 ttcatcagca gcgtcagggc ccccagacgg cggtgatgca taatttcatc gtcatccatc
     1501 accgtcacat ccaccagatg gaacgctctg gtgtagattt tagccacaat atccgggtca
     1561 gaaaagccat ccagccagtt catgctgtac ggatacggac tgcgctcacc gtggtagaac
     1621 agcaccggta tcaccagcgg aagtacctta tgcgcctcaa cgtgtcgctg catcgcagcc
     1681 accgcatacc tgagcattct gaacgccata tacgcgtttt cggtactctg gtgctccaca
     1741 aggcagtaga cataaccatc ctcaccgctg acggtcttca tactgaacag tgcatcacac
     1801 gcatactgct tcaggtcctg gtccacaaac gtacagggct ccagccgcag cgtcgataaa
     1861 tcacacaact gacggtattc ctccggaaga tgcagcgaga caaaatcccg ggccacctcc
     1921 ggcgacgcca taaacgccct gaaggcggca tcatgcggcg tggacgtgtt tttatccttt
     1981 ttccggctca cggtaacctg cctgactctc ccgggttatt tttcaccgga attattttac
     2041 tgatatcatg ccccggcaca acagcacaaa atcacatgcc ggaacaggat accgtctccg
     2101 gtgagcatga gggacaatac cagtgggaac actgagcatt gaccgtaaac cagagggaat
     2161 ttacaacagg tcacataaaa ccgtgcagac aacaccacag ccgggtaaaa cagccacgga
     2221 atataaagtt aaacccgcat acctcacccg gcagaatgat aatcagaagg ccgctgataa
     2281 aacaccgtgg cagaatatga caaaacgcca gcgtaaaaac cgcaagcgta taaatcgtct
     2341 tgtggaactc tggcctgagt tattcagccg ggaaaaacca aaaccactta aagtggggat
     2401 attcgacgag ctgatacagg atatcgccat cagagaactg gcgtttggcg ctggtacatt
     2461 acgcgcggca gtggcgtctt atgtgcagtc tccgcgctat tatcgtgcgt taatggcagg
     2521 tggcgcccgt tacgacctga aggggcagcc gtgcggggag gtgacgccac aggagcagaa
     2581 agaggctgaa acccggctga tggcgctgaa tgaccggcgt aaacgtccgc gtagtgcaac
     2641 agaatgattc acaactacag aatcaggtta acaacctctg atgtaacaaa aaagcaacag
     2701 caccatcaga agatacgttt tctggtggta cgataatact ccggataatg cgcggattca
     2761 ccgggaggaa accagagcag cactctgcac cgggtgcttc gtgattccgc tcacaaccgg
     2821 cagcgcaaat gataacgcag ctgtttttaa cgcaacgtac tggccggatg tatcatcccg
     2881 ggcaacctga taacaccttt ccacccggca ttgtccgcct gcacttatct ccccccaggt
     2941 ctgttgcagt aaatccacca cttgcgaaca ttgttcatca actgtggcac tggcccgcgg
     3001 atggcaaatg ctcgtctggc tgagcagcaa caggcatcgt attgttgctc ctccatgttg
     3061 ttcccgcaac cagcgtaaca ccaccggtga ggatggactg gccgtgtgat tacgctcagt
     3121 aatacgttca taaaagaaag tacagaatgc atgaatctgt tgcccgttga ttccttctgc
     3181 cgaatggagt gtgaagtgga ctgccagcag acgcggccag tggtccaccg cctgctgaat
     3241 aaaacgccgg atatctcccg gctctgcaag taaggcttcg gttatctgca cgaaatgttc
     3301 cttttcaaac cgttgcattt tgttaagccg gtgcagacac acgggaaagc acacatcaac
     3361 accggcacag tacgatacaa acacccgcta ctggtgctat ggatggaatg acgaatcagt
     3421 caggaaagcg ctacaacctg ctggctgacc ggagaatgtc tgcaggaagg ttcacggtaa
     3481 tgtcgccagc accagggaca gggccgggct ccacaatacc agcgccggca ttttgcacac
     3541 tgccagcgca ccggcatcac aacacgtttc atgcaactcc ccctcgtgat tcctctgttc
     3601 gctgctgcat ccactgctcc acttcgcttc tgtacccgcg gctgctacga cccggtttta
     3661 tgggttcagg aaaatggcct tcactgatca gcctgtaaaa ccatttatcg gtcataccgg
     3721 tgaagctggt gatgtagttc atgtcgatca ggggatcgtc ggcacgcagc aatacgctgt
     3781 tgtggcctgt tattgaggtc agaatatcct ctgtgagatt gatgaaggta tacgggggaa
     3841 ctgctgaacg gtgctttcag agctggtctg ctacgggaga ccagggatta ccggcaaggt
     3901 aacgggcctc cggaacgcgc acaccacgac gatcgcaggc gaaatcactg aacacccata
     3961 tcacttttga gtctctcttc gcactccggt gaacgactgt acggcaccac atcccggggg
     4021 gagcggcttc tggtacggtg ctgcttcacc cggtgcggat tcaacgattc acggtgcgct
     4081 ttcgcatccc ggcacactga atcctccggg attctggtaa gcagaactgc agatatccgc
     4141 tgtcgcgtcg ttgcacggtg tgggtgcgaa acaccggata atatgcaggc tggtgatacc
     4201 tctgaacgtc ctgagcagcg cggactttgt cggatgacgt ggaaacacca cgtcaggggt
     4261 ccagcagcaa atctgaccgg tacctgtaaa catactttgt acataccgct ctgcaataaa
     4321 tccccgtacc acaggcctca cagaacttct tcccgggaaa aaacttgtcg cacatcacac
     4381 aatgccactc cagcacgact ggtaatggcg ataaaaacat cgccatatcc tcaatgtaag
     4441 ggtgggactt ttccggattc aggaccacac agtccgcctt ctgttgctcg ctcagggcat
     4501 gtaaatcgtg ctcaaaccac gccccctgag catctgtctg caaaatcaag cggccacgac
     4561 aggaaaggca gaaacaatgc cggatctttc tgctgagcgc agcgtcggca ctgataatgt
     4621 gctcacccgg cgtgatcccc agccccgttt ttataccgtt catcctgcca ctgcctcctc
     4681 actgaatcct ccggtctggc agtgtgtaca gtactgctcc ccataataag cgtggcgaca
     4741 ttgtctgcag taccagcagg ctttacgcac cacgggtaag gcatccggta cgaattgctg
     4801 cagacgttta atcagctgta cttccctgtg ttccggttgt acgtaagggc actcatgacc
     4861 gtgttccgtc agcccgtcgt cagtgtgttc aaaccaggga cgttccgtgt cgtgctgcgg
     4921 gtggtatctg agtacactgc cgcaaaggtg gcaggtgtag taagcataag gtgcggtctg
     4981 tgcggtacgg gcaccggtca gacgtccgtt gccatcaagt gcgagaaaag attttgcgta
     5041 catcgtcaaa ctcctcagcc gccagacgac acgcagacaa cagccgtccc ggtacgggca
     5101 gcgtggtcag ggtgtgaatg gcggttttca gttcagtctc agatgggaaa atcagtgtgg
     5161 tcgctgtgaa cgcgtcatca cactgaggtc agataagtcg tctgtcgggc tcagccacga
     5221 ctgcagccaa agttgcgctc accggtgccg taagctttgg tactctcctt cgccagatag
     5281 tcagcacgct ccagcacctg ctgaaagccg gtgtcatcat tacgcgccag ccacaccgct
     5341 ggcctgaccg gaaaatgcac cagcgtggta tgatctccgg tatccacacc cagggcactg
     5401 caccaggcct gtttaatcat cccggccagt gaccccggat cgcggtaatc acctgcacga
     5461 caccaggtat cccggttgac cagcagcagg tgatagtgtt ttttgccttt tagctccccg
     5521 aactcccggg cccaggcgta atgcagggtc gtgggatgca cgcgtttacc ttcacggcgc
     5581 ttacgcttct ggtaagcgtt gattcgggct ttcagggcat tgataaagcg ggatatcacc
     5641 gccgcgtccg ttgctgccgg tacatccggg agccgcaggt caacccgaag tgccgtcagg
     5701 cggggatgaa catccagtgc gcacagcacc atctcacgga tgcgttgttg ccagaagggg
     5761 ttgtatttgt aggtcatggt taaatctccg tatggttcat acggaatatc cacgccgtaa
     5821 aaaatgcgca gagtccctga cgtgacctcc ggcaggacaa tgcctcaggc actctgtgat
     5881 aacccgaacg gttgtctccg gactgacggt tgaattttca gggggtttta atgtaaaaaa
     5941 ccggttacgg gtaaggctgt gaggagggag tgatggtgat acgcaaaaag aagtgcaggg
     6001 agtgtgataa cgcgattact cataatacgg tgtgttgtcc gtactgtggt tctgtcgacc
     6061 ccttcggcta ttaccggaat acggacagga tcttcacggc cctgctggcg ctgctggttg
     6121 tggttttgct ggtgacatct gctgtcagca tgtatgtgct gtggtagcgg taacggggct
     6181 tatcagatat ccgtgagggt tgccagagtt cgtcccctgc aacgcgccgt caacccacac
     6241 gctgtaagcc gttaatcccc gaagcagacc accctgcccc ttttaatttc aaattattcg
     6301 ttgtcacgat accgcttaac gttcgcggta ttatccaggt taccaacaac cggagaagac
     6361 ctaaaaagaa tcacacagag cagataacac cagaccgttg tgattaccgg atactcaggt
     6421 gaccagactg gatccacacg cgtaaaattt gtcgtcagtt ctgccagccg atcgaccgca
     6481 gaaggcttcc ggctccgcag gggtaaggtt ataacgtgtg cgggaaatca ctttttccag
     6541 cgtctcccgg gatgaacaac gacggaactg ctctggtttt aaccgctatt agcctctcgt
     6601 tactttatgc tgtaatcaac gtgatactgc cagcataccg caccagactg acattctggt
     6661 accctcccca aactcattac tcccgtgcaa ccacttaacc ggtctctcag caatatcgcc
     6721 ttaacgctac gtttagttat ccgacaccgg atgtccgatg ccattgcgac aacctgaagg
     6781 taaccagcca gttagctgag atgttaagct gctggctgca tacaccttgc caaatctaat
     6841 cgctcttaac caatttccag gatacaaggg ggcgattact gagaatgctc tcagactgtc
     6901 actcaccaga tcggatacgt ttaccgctca ggcggatgct taatgcactc tgttcaggtg
     6961 cattgcaggt acgttttatg tacgcaggtt atacgccagt tatcagggat aaccctgtga
     7021 gtacaacgca atctgttgct actgggtaga ctggtctgct gatcacactc acagatttat
     7081 ttcacatata tgaactcatg tgtttgatct catgagtttg agctcatgta tttgatctct
     7141 cttatatatt tataatacca atcagacagt cttatcccac cagtgatatc tgtcgcctgc
     7201 ccacaggtca caggctgact caccatcacc gccttcaaaa acaactctgc aattatcaga
     7261 acgccagttt tccccttgtc tcacgaaagt cctacctcct ttatcgcgtg cggaagcatc
     7321 gtttcgcaac aatcacccga cataccggta cggctcagcc accgcaatcc ccccgtttat
     7381 ct
//
LOCUS       DAZFRE010000173         7238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFRE010000173 DAZFRE010000000
VERSION     DAZFRE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.174"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            239..1672
                     /gene="cscA"
                     /locus_tag="ACHKUI_004801"
     CDS             239..1672
                     /gene="cscA"
                     /locus_tag="ACHKUI_004801"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6600825.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWRDADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQKNAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHEHLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEKRELSLYASHGVAVLQHGALWQLG"
     gene            complement(1680..2675)
                     /locus_tag="ACHKUI_004802"
     CDS             complement(1680..2675)
                     /locus_tag="ACHKUI_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6600826.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTLHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFYM
                     ATGDDHYTDLESLLNAHFQSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRETRIPCPLLIRCST"
     gene            2899..3123
                     /locus_tag="ACHKUI_004803"
                     /pseudo
     CDS             2899..3123
                     /locus_tag="ACHKUI_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3141..4469
                     /gene="dsdA"
                     /locus_tag="ACHKUI_004804"
     CDS             3141..4469
                     /gene="dsdA"
                     /locus_tag="ACHKUI_004804"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO6600827.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAAAGGIIESELVAIPAMQKRLEKEYHQPIAGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GSQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4577..6115)
                     /gene="emrY"
                     /locus_tag="ACHKUI_004805"
     CDS             complement(4577..6115)
                     /gene="emrY"
                     /locus_tag="ACHKUI_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6600828.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVAFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6115..>7238)
                     /gene="emrK"
                     /locus_tag="ACHKUI_004806"
     CDS             complement(6115..>7238)
                     /gene="emrK"
                     /locus_tag="ACHKUI_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6600829.1"
                     /translation="KYFALLAVVLFIAFSGAYAYWSMELKDMISTDDAYVTGNADPIS
                     AQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDK
                     QYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAY
                     KANKALVMNTPLNRQPQVIEAADATKEVWLALKRTDIKSPVTGYIAQRSVQVGETVSP
                     GQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGKNVVFHGRVTGINMGTG
                     NAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEM
                     PDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1984 a         1708 c         1681 g         1865 t
ORIGIN      
        1 ggcattaata ctttggctgg catttttttc cctcaaattc atctgactta cgcatagtga
       61 taaacctctt tttcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa
      121 atgtcatact taacgttaac atttgtgata ttcatcgcat ttatgaaatt aagggacttt
      181 atttttataa aagttaacgt taacaattta ccaaatttgc ttaaccagga tgattaaaat
      241 gacgcaatct cgattgcatg cggcgcaaaa cgcactagca aaacttcatg agcaccgggg
      301 taacactttc tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa
      361 cggcctgatc tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagtga
      421 acactggggg ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca
      481 tgagcctatt gcgctagcgc ctggagacga gaatgacaaa gacgggtgtt tttcaggtag
      541 tgctgtcgat gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg
      601 tgcaggtaat gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat
      661 tcatttcgag aaacagggtg tgatcctcac tccaccagaa ggaatcatgc acttccgcga
      721 tcctaaagtg tggcgtgacg ccgacacatg gtggatggta gtcggggcga aagacccagg
      781 taacacgggg cagatactgc tttatcgcgg cagttcattg cgtgaatgga ctttcgatcg
      841 cgtactggcc cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag
      901 ccttggcgat cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag
      961 ttatcgaaat cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt
     1021 tgcacaatcc gggcatttta ctgaacttga taacggacat gacttttatg caccacaaag
     1081 ctttttagcg aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc
     1141 aatgccctca aaacgtgaag gctgggcagg ctgcatgacg ctggcgcgcg agctatcaga
     1201 gagcaatggc aaactcctac aacgcccggt acatgaagct gagtcgttac gccagcagca
     1261 tcaatctatc tctccccgca caatcagcaa taaatatgtt ttgcagaaaa acgcgcaagc
     1321 agttgagatt cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca
     1381 actcggcaca ggaatgcggc tgtatattga taaccaatct gagcggcttg ttttgtggcg
     1441 gtattaccca cacgagcatt tagacggcta ccgtagtatt cccctcccgc agggtgacat
     1501 gctcgcccta aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga
     1561 ggcggtaatg agtagccgaa tatatccgca gccagaaaaa cgggaactgt cgctctatgc
     1621 ctcccacgga gtggctgtgc tgcaacatgg agcactctgg caactgggtt aacataatat
     1681 caggtggaac aacggatcaa cagcgggcaa gggatccgcg tctctcttcc cccttcacga
     1741 ccttcaataa tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg
     1801 gtcagcggcg gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg
     1861 acatcctgcg ggatacgtac ccccttcgcc agaagaacct gataagccac aaaggctgcg
     1921 cgatcgttac cacatatcag aacatcaaaa tctggtttgc cagattggaa gtgggcattg
     1981 agtaaacttt cgagatcggt gtagtgatca tcacctgttg ccatgtaaaa ttgtttcacc
     2041 tcagccagat ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct
     2101 gttgccaacg cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc
     2161 aaatgcagtg ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct
     2221 gggtcatccg ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc
     2281 gtgatatgtc gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc
     2341 tgacgtgccg cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag
     2401 ccgaactcgc tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta
     2461 gcagtgtcct gtgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatttta
     2521 cgggcggaaa gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc
     2581 gttgcaggac gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact
     2641 cccgccaggc gtgcgacatc ctttaatgaa gccataccca agccgtttgc cgtaaaacgg
     2701 gcactgtagc agaaacagac gtcactggcg agatccaacg atattctcga cgccctatca
     2761 cccgacacgg caatacaata aaaagtaaca ataattcccg gacaattgta cccaatttcg
     2821 cctctgttct cgccaacgag tcaccatgta ggcaagtagg taaaacatgc caacctggag
     2881 aagaagatat aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt
     2941 attgcgattg ctatcggttc cggtgcgatt ggctgcacga tcgttacgga ctcgcttttc
     3001 tggctggtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
     3061 gcgacattta tcgcttcagt catcgctctg gcgggcacat tcctgctgtc atttatcatc
     3121 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ttcgctcatc gcccagtatc
     3181 cgttggtaga ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct
     3241 cattggcaga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
     3301 cgcgcctgtc tcgttttgcg ccgtatctgg caaaagcatt tcctgaaacg gctgccgcgg
     3361 gggggattat tgaatcagaa ctggttgcta ttcctgctat gcaaaaacgg ctggaaaagg
     3421 aatatcacca gccgatcgcc gggcaattgc tactaaaaaa agacagccat ttgccgattt
     3481 ccggctccat aaaagcacgt ggcgggattt atgaagtcct ggcccatgct gaaaaactgg
     3541 ctctggaagc gggtttgctg acacttgagg atgattacag caaactgctt tccccggagt
     3601 ttaaacagtt ctttagccaa tacagtattg ctgtgggatc aaccggaaat ctggggttat
     3661 caattggtat tatgagcgcc cgcatcggct ttaaagtaac ggtgcatatg tctgctgatg
     3721 cccgcgcctg gaaaaaagcg aaactgcgca gtcatggcgt tactgtcgtg gaatacgagc
     3781 aagattatgg tgttgctgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
     3841 tctttattga tgacgagaat tcccgcacgt tgttccttgg atattccgtc gcaggccagc
     3901 gtcttaaggc gcaatttgct cagcaaggtc gcatcgtcga tgctgataac cctctgtttg
     3961 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg
     4021 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac acactccccg tgtatgttat
     4081 taggcgtcca taccggatta cacgatcaga tttctgttca ggatattggc atcgacaacc
     4141 ttaccgctgc cgatggcctt gcagttggtc gcgcatcggg ctttgtcggg cgggcaatgg
     4201 agcgtctgct ggatggtttc tataccctta gcgatcaaac catgtatgac atgcttggct
     4261 ggctggcgca ggaagaaggt attcgtcttg aaccatcggc actggcgggt atggccgggt
     4321 ctcagcgcgt gtgcgcatca gtaagttacc aacagatgca cggtttcagc gccgaacaac
     4381 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
     4441 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg ccatcctttc
     4501 cctgggtgag cgatgctgcc gatgacgcag acttaagatt ctgccgtttt acctgctata
     4561 gcctcctctt tttccatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
     4621 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
     4681 tagaaagtga ctgctgagtt atttcattat taatttcatt aagaactcct gaaagcgaac
     4741 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
     4801 ttgctgtcaa ctgactatgg tgtaacgatt cgcggcgtcc ccacagtgtc attgtcaacg
     4861 acgtaccaac tgatcctgac aaggtgcgaa aaaaattact catactcgag gcattggcaa
     4921 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
     4981 cggcgaatcc ctgaaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
     5041 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactaaat gccaccaaca
     5101 accgcatatc aattttgttg ccataacgtc ctatcaaagg tgatattaat agcggcatga
     5161 taccgatggg cgcataggca agtccggccc atatcgcatt ataccccatc gtttcctgga
     5221 gtaactgcgg cataagaact atcgctccag agtaaaataa atacgcgcat gtgatactca
     5281 caataccaat ggtgaaatta cgggacttaa acaaactgag gtcaagaatc ggattctctg
     5341 aggtcgactc ccaaatgact aaagagatca gagaaataac tgatactact gttagtagta
     5401 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
     5461 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagtct
     5521 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
     5581 cattgattaa aaatatccaa ccccagctaa agttatcgca aatatagccg cccaatatcg
     5641 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
     5701 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
     5761 ttaacccctg aacgactcta aaaaatatca gcacatctag attggtcgat aaactacaca
     5821 ttaatgaaga cagcgaaaaa aaggttactg aaagtaaaaa taaccttaat tcgcctattc
     5881 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
     5941 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
     6001 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtga
     6061 cgcaccataa cgtcccaccg gttaatggtg caggagttga tttagtgatt gccattaaag
     6121 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
     6181 taaagcctta ctggtataag caggcatgga cgtcacggtt gaagccagat cgggcatctc
     6241 ggcaatgtct tcattcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgcaaagg
     6301 gtgttccatg agttcttttg gatccagaga aacttcaacc ggtacacgct gaacgatttt
     6361 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
     6421 attgatccct gtcacccgac catgaaacac aacattttta ccataaagat cgctgataat
     6481 attgaccgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
     6541 ccacatttga cgcgccggta cgacagccat taatgattgt ccgggactca ctgtttcgcc
     6601 gacctgaaca cttctctggg caatataacc ggtaaccgga ctcttaatat ccgtacgttt
     6661 aagcgccaac cagacttctt tggttgcatc tgcagcttca atgacttgtg gctgacggtt
     6721 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cagcattcaa
     6781 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgcttctt ttgaaataac
     6841 cccttgcttc gctaaaggca cacgacggtt ataatcttct aaagattgtt gatactgaat
     6901 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtatagtt tattcgtttg
     6961 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
     7021 atccagtgaa actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac
     7081 agtgacgctg cctgagactt gtgcagaaat tggatctgca tttcccgtga cataggcgtc
     7141 atctgtacta atcatgtctt ttaattccat tgaccaatag gcataggcac ctgaaaacgc
     7201 aataaataaa actaccgcta acaaagcaaa atattttc
//
LOCUS       DAZFRE010000174         7141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFRE010000174 DAZFRE010000000
VERSION     DAZFRE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.175"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..401
                     /locus_tag="ACHKUI_004807"
     CDS             <1..401
                     /locus_tag="ACHKUI_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6600830.1"
                     /translation="RGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            456..941
                     /locus_tag="ACHKUI_004808"
     CDS             456..941
                     /locus_tag="ACHKUI_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6600831.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            957..1433
                     /locus_tag="ACHKUI_004809"
     CDS             957..1433
                     /locus_tag="ACHKUI_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6600832.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1496..1717
                     /locus_tag="ACHKUI_004810"
     CDS             1496..1717
                     /locus_tag="ACHKUI_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6600833.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1736..2380
                     /locus_tag="ACHKUI_004811"
     CDS             1736..2380
                     /locus_tag="ACHKUI_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO6600834.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            2430..2798
                     /locus_tag="ACHKUI_004812"
     CDS             2430..2798
                     /locus_tag="ACHKUI_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6600835.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCDYVYLAVYPTPEMKN"
     gene            2887..3264
                     /locus_tag="ACHKUI_004813"
     CDS             2887..3264
                     /locus_tag="ACHKUI_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6600836.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            3261..3749
                     /locus_tag="ACHKUI_004814"
     CDS             3261..3749
                     /locus_tag="ACHKUI_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6600837.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASTECLPDLPTF
                     DW"
     gene            3766..3963
                     /locus_tag="ACHKUI_004815"
     CDS             3766..3963
                     /locus_tag="ACHKUI_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6600838.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            4048..4893
                     /locus_tag="ACHKUI_004816"
     CDS             4048..4893
                     /locus_tag="ACHKUI_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6600839.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            4962..5357
                     /locus_tag="ACHKUI_004817"
     CDS             4962..5357
                     /locus_tag="ACHKUI_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HFO6600840.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKGQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
     gene            5350..6283
                     /locus_tag="ACHKUI_004818"
                     /pseudo
     CDS             5350..6283
                     /locus_tag="ACHKUI_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            6700..6870
                     /locus_tag="ACHKUI_004819"
                     /pseudo
     CDS             6700..6870
                     /locus_tag="ACHKUI_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1910 a         1830 c         1735 g         1666 t
ORIGIN      
        1 accggggaaa tgtggtggac agagtcattg aaggggctta cgaggtagtg ggcgtgtttg
       61 accggattga ggaaaagcgt gatgccatgc agtcgctgat tctgccgcca ccggcacgcc
      121 aggcgctggc acaggcggca ctgacttacc gttatggtga cgaacatcag cccgtcacca
      181 ccgccgacat tctgacgcca cgacgccggg aggattacgg taaggacctg tggagtgctt
      241 atcagaccat tcaggagaat atgctgaaag gcggaatttc aggtcgcagc gctaaaggaa
      301 aacgtatcca tacccgtgcc attcacagca ttgacactga cattaagctc aaccgcgcat
      361 tgtgggtgat ggcggaaacc ctgctggaga gcctgcgctg attcagccat taccctgaaa
      421 accacgcatt tatttgtcct ttaaggagtc atttcatgaa aaccatttca cataattcca
      481 ccacatcttc tgtttccgta accgctgcat cagggaatga ccagcctcag ttggttgcca
      541 ccatagtccc tgatgaacag cgcatcagct tctggccgca gcattttggc ctcattccac
      601 agtgggtggc cctggagccc cgtgttttcg gctggatgga ccgtctgtgc gaagactact
      661 gcggtggtat ctggaatctg tacaccctga acaacggcgg ggcatttatg gcccccgaac
      721 cggatgacga tgatgacgaa acatgggtac tgttcaatgc catgaacggc aaccgcgctg
      781 aaatgagccc ggaagccgct ggcattgctg cctgtctgat gacgtacagt catcacgcct
      841 gtcgtacgga gtgttatgcc atgacggtcc attattaccg gctgcgggat tacgccctgc
      901 agcatccgga atgcagcgcc attatgcgca tcatcgactg aatggaggca gggacaatgc
      961 agcagctttc ctttctgccc ggagaaatga cgcccggcga gcgcagtctc attctgcggg
     1021 ctctgaaaac cctggaccgc catcttcatg aacccggtgt ggccttcacc tccacccgtg
     1081 cggcacggga atggctgatt ctgaacatgg cgggactgga gcgtgaagag ttccgggtgc
     1141 tgtatctgaa taaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca
     1201 accgcacgga agtccatccc cgggaagtga ttaaacgcgc cctgtaccac aatgccgctg
     1261 ccgtggtact ggcgcacaat cacccgtccg gtgaagtcac acccagtaag gcagaccggc
     1321 ttatcaccga acgtctggta caggcactgg gcctggtgga tatccgggtg ccggaccatc
     1381 tgatagtcgg tggcaaccag gttttctcct ttgccgaaca tggtctgctt taacccgtca
     1441 caaccacatc acacctgttt tcacttttat cttctgtctt cagaggtatc ccattatgaa
     1501 aattatcact cgtggtgaag ccatgcgtat tcaccaacaa catccgacat cccgtctttt
     1561 cccgttctgt accggcaaat accgctggca cggcagcact gaagcgtata ccggtcgtga
     1621 agtgcaggat attcccggtg tgttggccgt gtttgctgaa cgtcgtaagg acagttttgg
     1681 cccgtatgtc cggctgatga gcgtcaccct gaactgaatc aggacgggca ttcagatgag
     1741 cagcattatc accatcacag tgtatacgct ggatgaactt tcctgcccgg cacggggaaa
     1801 agcccgcgac tggtaccgtc agcatcatgc tgacagcaac tggtatgaaa acgtgtatga
     1861 agacttcagg gaggtctgtg gcattttcgg gattgacctc cgccagcgcg ttttccggct
     1921 cagcaatggc aggtttatgg aagagccgtg catctggttc agcggattct gcagccaggg
     1981 ggacggagcc tgttttgaag ggcgctggca ctggcagcca gcgacacccc ggaaaatcag
     2041 ggaatatgcc cctcaggacc gtgagcttca tcgtatcgcg gataccctgc aggcggtgca
     2101 gaaacgtaac ttctggcagc ttcaggctga aatcagtcac cggggacgtt actgccatcc
     2161 gtacagcatg gatatcaccg tcacccggaa cagtccgact ggtcaggcaa tgacaaccga
     2221 tgcggaagcc gctgtcagcg aagccctgcg cgacctggcc ttctggctgt accgccagct
     2281 ggaaaacaag tacgactggc tgacctcaga caccgctgtg gatgaggccc ttctcatcaa
     2341 cgaatatacc ttcaccgaag ccggtctgcg cgccggctga cccgtcatcg ttaaactatc
     2401 attcaccgat tttctgtgag gattttatca tgtcggacac actccccgga acaacgcttc
     2461 ccgacgacaa caaagacctc ccctggtggg ggctgccctg caccgtgacg ccctgtttcg
     2521 gggcctgtct ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc gccggtatca
     2581 gaggccggtt cagcgatgcg gatgcgtacc atccggacca ggcctttccg ctgctgatga
     2641 aacaaccgga actcatgctc accagcggtg aactgaatcc ccgccatcag cataccgtca
     2701 cactgtatgc gaaaggactg acctgcgaag ccgataccct gggctcctgt gattacgttt
     2761 atctggctgt ttatccgacg cccgaaatga aaaattaact ctccagaata gccttctgct
     2821 acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat caggaaaatc
     2881 ttcagtatga aaacattacc cgacacacac gtacgggcgg catcgcgctg cccgtctccc
     2941 gtcaccatct ggcagacact gctcacccga ctgctggacc agcactatgg cctcacgctg
     3001 aatgacacac cgttcgctga tgaacgtgtg attgagcagc atattgaggc agggatttca
     3061 ctgtgtgacg cggtgaactt tctcgttgaa aaatacgcgc aggtacgtac cgaccagccg
     3121 ggattcagcg caggagcccc gtcgcagtta atcaacagca ttgatattct ccgggctcgc
     3181 agggcgaccg gcctgatgac ccggaacaat tacagaatgg taaataacat tacccagggc
     3241 aagcatccgg aggcaaaacg atgaaactgg ccctgacgct ggaagctgac agcgttaacg
     3301 tacaggcact gaacatgggg cgaattgtcg ttgacgtcga tggtgttgag cttgccgaac
     3361 tgattaacgt ggtctgcgat aatggctact cccttcgtgt tgttgatgaa tctgaccgga
     3421 cctcaacaga cagcataccc ccctctgctg ccctgagcgg tatacgctgt agtaccgcgc
     3481 atatcacgga aacagacaac gcctggctgt actcgctgtc gcatcagacg aatgacaccg
     3541 gcgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc gatgcgtggt
     3601 catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgc cgtctggttg
     3661 tcacacttac ccgacgttat ggcgtcagtc tcattcatct ggatgccagc actgaatgcc
     3721 tgccggattt acccactttc gactggtaac ccggaaattc acaacatgaa atcattaacc
     3781 acggaaacag cgctgtatat tctgattgcg tggctgcagg acaatattga ctgcgaatca
     3841 gggattatct tcgacaacga tgaggataaa acggattcag cagcactgct gccctgcatt
     3901 aaacaggcca gggaagatat ccgtaccctg cgccaactgc agcttctgca acagaaccgg
     3961 tgagtcttac tcaccagact tcattccact gacgccagcc tgaatacggc tggcgttttc
     4021 gtttataaac caaaaggaaa aacgatgatg tcagatatca caatctcccg cccggaactg
     4081 gtcaacggac atacggacgt tatctgctca accagtatcc gccacattct ggctgtacga
     4141 aagagtgcgc tgctgcaaat cgacacactt atccggcaac tggcagaaat ctcagtactg
     4201 acagaaagta ttggaggtaa aaccgccctg gactgggcga tgaaacagga ttttcgctgc
     4261 ggctgctggc tcatggagaa accggaaacc gcaatgaaag ccatcacccg caatctcgat
     4321 cgcgaaatct ggcgtgacct gatgcaacat tccgggatgc tgtcattaat ggatgcgcag
     4381 gcccatgata catggtaccg ctcactggag tacgataact ttccggaaat cagcgaggcg
     4441 aatatcctga gtacgtttga acaactgcac cagaataagg atgacgtgtt tgagcgaggt
     4501 gtgatcaacc tcttcagagg gctgagctgg aattacaaaa ccaattgccc ctgtaaattt
     4561 ggcaataaaa ttatcgtcaa caatctggtg agatgggacc gatggggatt gcatcttatc
     4621 accgggcaac aggcagatcg actcgctgac ctggaaagaa tgctgcatct gttcagcggc
     4681 aaaccgatcc ccgacagccg ggaaaatatc atcattcggc tggatgatca catccgctcc
     4741 gttcagggca aagcgtgcta tgaagatgag atgttcagca tcagatactt taagaagggc
     4801 tccgcacaca tcacgttcag gaaaccagaa ctggttgaca ggctaaatga cataattgcc
     4861 aggcactatc caaatgtatt agcagacagg taatgtggcc ttgccccgct attaaacatg
     4921 tcatattttt gctatcattg tgaaaatttt tcggaggaaa catgactaca aaagaccgta
     4981 ttcagtcccg actgaaccgt tcaaaacgtt atgtgttcac ccgggatgat ttcagggata
     5041 tcgctggcta tgatcaggtt ggcagagctc ttcgtacact ggtaaacgaa ggtaagttga
     5101 tgaaagtggg ctatggtgtt tacaccaaag cccgtcgtaa catcatcact gggaaaatca
     5161 tgcccgcagc gcctgggggc tctgctgcag taattacaga ggccctggag cgcctgaata
     5221 tcagataccg tctggcagga gcaacggaat gctacaataa aggtcagtcc acacaaattc
     5281 ctgtatctct ggggataaaa acaccttctc gatttaaaag aatgttaata gtagggaaaa
     5341 gtaaaatcaa tgcctgaatt aacatcatta tttcctgacg ttgccgatgc actggatatt
     5401 gaatctgtcg ctatcgtcga aaaagattat tttgtggttg atcttttaag attattaaaa
     5461 gaaataaaac cagaaacaca tactctcgtg tttgcaggag gtaccgcatt atcaaaagca
     5521 ggtatctcgc taaacagaat gtcagaagat attgatataa agctcgtgcc aacagagaac
     5581 tttatgcaaa atggcagaga caagagaaga aaaattcgca aggaaatcgt gcaaataatc
     5641 acggatgtta ttcataattc tgacattttc agcttggata atgaaaacgc ccgtataaca
     5701 cgtgatgagt atcgttataa cgaaatctcg gtacgttatc cccagacatt tgcacaagtc
     5761 ccctgtcttc gtccatttat taagctggag ttgatggaaa gcactctgct ggaacatccg
     5821 gaaagcagag atatttattc ccttgtcacg gagttaacgg gtaaaggtac acctgtaaca
     5881 gcattcccct gtgccaccat tttatccact caggctgaaa agctcatctc catgatgcga
     5941 agaacagcag cacatcttcg taatcccgaa caacaggatg atgagtttct ggtacgtcac
     6001 atttacgaca actactgcat tgtcagggaa aaaggtgtga atgttcctgt attgaaaaat
     6061 tttgttcaga tatgtattca gctatggtaa ccagtaccca caattttgta gctcaccagt
     6121 tgatgaactt aagaaagggc tggatgcact ccgggattat cctgtacaca aattgcgctt
     6181 tcaacgattt gttaaaccga tggtatttgg taatacacag attaactggg aagaagccta
     6241 ttccagtttc agacaaacag cgctaagtgt tctgaccagc taacaaacgc gttaagtacg
     6301 attccacaat tcagcatatc gaaccagacc aaatgattac agcaaagcac aaaaaagagg
     6361 gattatagcg tcaaaagaat tcgaattgca catgggggcc tcattggtgg cctcaaaaac
     6421 aacaaataca aaataatcta tataaaacaa tttgttatac aatcagtttc attccgattc
     6481 cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt tggatcttta
     6541 tataaaataa acttgggtgc ataacgccat caggtcttaa acactcccga atcatcaata
     6601 aacatccccc gctaacaaaa cacagttcag ttcacataac atccctgtta tcccacacat
     6661 atctgctggc cgaacgacgc cagtgcgcgt acggaggcaa gagcagccct gcacatggca
     6721 gcgcttgtgg taacctggtt taatccggtg aaagaggcgt tttacatgca tctgccagca
     6781 gccggaaaaa ccaaaaaagt ggcgctggtt gactgtatgc gtaaattgct gaccatcctg
     6841 aacgcaatgc tcagaaagaa cgaagagtga aatgaatcgt agcatcatgt tgctccataa
     6901 tttttacgtt caagacagtt gattatcggc gcatatgact caaaaagagc ctgatgattg
     6961 cctcttttcc ataggctctt tctctgaagt gaaggaaggc ccggaagaaa gcactcgctg
     7021 gcatacgtga ctccagctca accggagata atcaccattg ttatttttct atttaataat
     7081 aatctcctga aaaatagata tagcaaaaat acaatccggc cagatttaat tccgcattca
     7141 t
//
LOCUS       DAZFRE010000175         6812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFRE010000175 DAZFRE010000000
VERSION     DAZFRE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.176"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            359..1843
                     /locus_tag="ACHKUI_004820"
     CDS             359..1843
                     /locus_tag="ACHKUI_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6600841.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2165..2767)
                     /locus_tag="ACHKUI_004821"
     CDS             complement(2165..2767)
                     /locus_tag="ACHKUI_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600842.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVFWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(2862..3068)
                     /locus_tag="ACHKUI_004822"
     CDS             complement(2862..3068)
                     /locus_tag="ACHKUI_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6600843.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3387..4376
                     /locus_tag="ACHKUI_004823"
                     /pseudo
     CDS             3387..4376
                     /locus_tag="ACHKUI_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4615..5973)
                     /locus_tag="ACHKUI_004824"
     CDS             complement(4615..5973)
                     /locus_tag="ACHKUI_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6600844.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            6255..6386
                     /locus_tag="ACHKUI_004825"
                     /pseudo
     CDS             6255..6386
                     /locus_tag="ACHKUI_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1671 a         1639 c         1777 g         1725 t
ORIGIN      
        1 tgcaggaact gtagtcttct ttattttatt cttcttcagg agggggggtg attcctgaag
       61 gcgttattcc cggtgttctg tttatgattc ttcccggcag gaaaccggta tctgcaatac
      121 ccgggatgat gctttttgtt cgtacgtggt tgtgtaaaat ttgaatagtg gatgtgctcc
      181 gaagggaagg aggggacaca cagattcatt ttgtctttct tcagcagcaa aatacactgt
      241 ggttattatt taaccattaa atcaacaaaa aattttacct catattgatt tgactgctat
      301 ttttgctatt aatagccaga attgcagtgc ttttatatct gtgtatagtg aggttactat
      361 gtcattcggt cagccttgtg atgagtttcc cctgagttcg ctgcctccac tcattcgtga
      421 tgccgttatt gaagctcagc aaattacgca ggctcccctt gggcttgtgg cagcgtctgc
      481 cctgggcgct gtttcacttg tctgccagaa tctgattgat gtctgcagac tgaatacact
      541 gcgggggccg gtctctcttt ttttcctgac gcttgccgag tccggagaac gaaaaaccgc
      601 agtggacaaa ttattaatga agcccctgta ccagcaggag atgcagttat attccagata
      661 caaaagtgaa ctggctgtct ggaaaaacaa ggaagagctt ttaaaggctc agaaaaaagc
      721 attgttgtca aaactgaata aagaactccg caaaggcgcg gatgagtcag aaaccctcag
      781 acaactggaa gtacttcaga aaaacagtgc ggaagaacct gtgaggtata aattcatttt
      841 taatgatgcc acgactgcgg caattaagaa tcagctttgt ggtaaatggc gttcagttgg
      901 tattatgtca gatgaagccg gtattatatt tgatggatat accctgtctg agttgccttt
      961 tattaacaaa atgtgggatg gctctgtatt gtctgtggac agaaagaatg aacccgaaca
     1021 gatgattgaa aatgccagaa tgacgttatc cctcatggtg caacccggtc tttttgaccg
     1081 atatatggag cgaaaaggtt ctgttgccag ggacagtggt tttcttgccc gttgtcttat
     1141 cagtaaacct gcaaccacgc aggggaaacg ctttattaac ggagctgtga ttccgggagg
     1201 atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta ttgaaaaatc
     1261 cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa tatttattga
     1321 gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc cgtttcgggg
     1381 acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc agtatttcag
     1441 ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa taagttcgtg
     1501 gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac tgcagcagaa
     1561 ggacgccgag gagctgttcg actggctcat tgaggagtgc cgcggagaat gcccgccccg
     1621 tgtcaggaaa aattatattc tccagtgtgg cccgggccgg ttcagaaaca ggaaaaaact
     1681 gaacgcattg ctgaacattc ttgaatctca gttcaggctt tctgtcgtgc cggaaggaaa
     1741 aaccatgtat gttctgcttc cccagatagc cagtttaaag ttgtctgatg tctctggtat
     1801 ttttacctcg ggttatcact acaataagct gagagcgaag taaccggtga gcagtttttc
     1861 tccattatgc ttcagactta gatgattgat tattatgggg atttcatcac cgatattcag
     1921 atgtatgaat atcggtgatg aactaaagct caggagctgc gtggtggtga tgaatcaaca
     1981 ggcatataac tgatacccgc cggtcagaag ttccagcctg cgactttcct gtctgcgtgc
     2041 ccgcagtccg gcgttcaggt gcagcgtgtg acagaacgca cggagagcga aacggcaggc
     2101 agacaggccg ttcagtcagc cctggcaccc tgcccggata ccagcttccc tgaaacaact
     2161 ggcatcaggt gcgcttatcg tcaaccgttg tgcggtatga caggagcacg tactgatcca
     2221 tcagatccgt cagtgctgcg ctctgcttct gacggttgat gatccattcc tccagctctt
     2281 tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag gcagtttccg gtcattaaat
     2341 caagccagca aagccagacc agtttgaggc gcaactccgt gccaccaaac tgacggaaga
     2401 ggctgatcag tttatttgcc agagaacgct gcctcagacg ttcacattca gacaggagaa
     2461 tgccttccag catcggatga tgaaccagca gatgttcact ggtgcataaa ttcactatca
     2521 gatcatcatc caggggcggt gtggcccgtt tgtcagggcc ataaatcagc cggtgcaggc
     2581 tctggggtac gatggcaatc tccggttttt caccattgaa tgacacccaa ccgggcgtca
     2641 gattatggaa gtagtagata ttcggatgac acacgcccca gaatacggcg tgccgggtga
     2701 agccggtcag ccatttctgg tagctttcgt ggagccattc gtagcgggtc tgatactgtt
     2761 gtttcatttt catattcctc acggggatat aacaggcagc agaatgccgc cggcaggccg
     2821 gagattacgt ctgctgcggg tgaacggata acggtcagaa attacggacg ggactgtgca
     2881 atacgcgcct gcagccaggc ttccacttca cttttcagcc agcgggagct gcgccccatt
     2941 ttgatggggg caggaaagcc cccatctttg atgagtttgt aaaaccactt atcggttaag
     3001 ccggtaagtt gagtgataaa cgccatgtcg accatctggt catccatcag cgaaacaggg
     3061 gtagccatga gaatgttcct ccggatgttg ttaaaccagc cggtgtggtc atgaaaacag
     3121 cgcgccacct tcactggcat aacaaccggg gagaaacgta aaaaataatc gggaatgtgg
     3181 ctgtccggag ataaacgggg ggattgcgat ggcagagccg tagcggaatc ccggggggtt
     3241 gttgcgaaaa gatgcttccg cacgcacgcg cgataaagga gggaggactt tcgtgagaca
     3301 aggggaaaac tagcgttctg ataattgcag agttgttttc ggaggtggtt acagtgagtc
     3361 agcccgttcc tggcgaggag gagactgata ccgccgaaca gctggagttg atgcgtagcg
     3421 ccttccgggt tatccggacg gtacgggaaa aacatgcctg tactcagtgc gatgccatcg
     3481 tgcaggcacc tgcaccttcg cggcccatcg agcggggtat cgccggaccg gggctgctgg
     3541 cccgcgtgct gacctcgaag tatgcagagc acaccccgct gtattgccag tcagaaatat
     3601 acggccggca aggtgtggag ctgagccgtt cactgctgtc gggctgggtg gatgcatgct
     3661 gccggctgct gtctccgctg gaagaggcgc ttcatggcta tgtcatgact gacggcaaac
     3721 tccatgccga tgataccccg gtccaggtac tgctgccggg taataagaag acgaagaccg
     3781 ggcggttgtg ggcgtatgtt cgtgatgacc gcaatgccgg gtcagcgttg gcacctgcag
     3841 tgtggttcgc ttacagcccg gacagaaaag gcatccatcc gcagactcat cttgcttgct
     3901 tcagcggtgt gctgcaagcg gatgcgtacg ccgggttcaa cgagctgtat cgcaatggtg
     3961 ggataacgga agctgcctgc tgggctcatg cccgccgaaa gatccacgat gtgcacgtcc
     4021 gcatcccgtc agcactgacg gaagaagccc tagagcagat cggtcagttg tacgccatag
     4081 aggcggatat aaggggaatg ccggcagagc agcggcttgc tgaacgtcag cgaaaaacga
     4141 aaccgctgtt gaaatccctg gaaagctggt tgcgtgaaaa gatgaagacc ctgttcttcg
     4201 gctctggcca tggtggtgag cggggagcgc tactgtacag cctgatcggg acgtgcaaac
     4261 tgaatgacgt ggatccagaa agctaccttc gccatgtgcc tggcgtcata gcagactggc
     4321 cggtcaaccg ggtcagcgaa ctgcttccgt ggcgcatagc actgccagct gaataacaca
     4381 tccccgtcaa tacggccctc gctgtacgct tacttttgtt cggactgctt atggcctttt
     4441 tccactggtg tagcaggaaa gttaaccggc tgccaccatg agatgacgtg taaaccgtgc
     4501 aggcatgttt tcacgtggtg acgctttcat tgggggtcta cgccggaacc ccaatattgt
     4561 tcacatacat gaatttgaaa aactaagcga tactgctgtc cgtaccttat aaacttattt
     4621 caacgcttcc ggcagcgtca caatccacag ttcactgtca gactccggtt tctccagtgg
     4681 cttcactctg agggtcgttt caacctgctt gtcgtcaagc gtcagctttc cttccgcgtt
     4741 cacccgataa tccttgaatg tcttgccctc gaccggatgt tgcatcgcca ccttcacgcc
     4801 aaagtcgtta tcgttcgcca ccgccagcgt tttgctgtcg atcagcgcca acccttcagc
     4861 cttctcctgc tgccagccca aggagcgcag atcaacaacc tgcgttttct gcgcaagggt
     4921 aatgccgcgc tgtgacaggg ttttctcatc atcaaactcc ggatactcac ccggcttgtc
     4981 gaagcctgac aggtcgctcg ccttattgag atcgacctcg taaatcaggt tacgcattct
     5041 gttgttttta tctcttccct gttcgatcag caggatgtgc tggttatcga gcgccacgat
     5101 gtctccgatt ttagcgtcgc tgtttttgct gtaggccgcg ctgtcgatag gataaccgta
     5161 catcgcggtt tttccgcttg ccggatcgaa gctcaccaga cgcgtaaaca gcgccttttt
     5221 cttgctctta gcgtcgatat ccagcgtact ttgcacggcg acaataatac gcccgtccgg
     5281 catacgggtg aggccttcaa agccgcggtt cgcctgacgc catttgagga tatttggcaa
     5341 accgcccgca atcgcttttt ccccttccgc cgcctgcgga ccgtggattg ccaggatttt
     5401 ccctttactg tcgatgttaa tcaggaacgg gccatactca tcgcacagcc agtaacctcc
     5461 tttaccatcc ggagtgatcc cttccgtatc cagcccgcga ttatccccct tcagtctatg
     5521 taacgtgtcg ctgaaggcca cttcattggt ggagccaatc acgtcgcttg ccagcggcag
     5581 accgttgatc gcgcctttat cgtcatgcag aggacgggga tcgatggcct ccgctttgcc
     5641 gttttgtaca cgaatcgtca tcagcagcgg cgcgaaatct ggcgtaacga aaattttagt
     5701 ttcgtttttc ccctctttcg gtgaatccgc gttcggaccg cgatcggtaa tggttgcgaa
     5761 tgtcagggca tcgccctgtt taccggtgaa cagcaggcca gaacctatcc caaccggaag
     5821 gccgttcggg aatgcactgg caaatgcgcc ggcgtagttc acgtgcgtgc cttctggaaa
     5881 actgacgaca tagcgctcag ccgtaggttg tgccgccagg gcagagaaag agagcgtaca
     5941 accgattagc acgggaataa tttttctttt catattgtta gcatccataa agtgaagatc
     6001 acactacacc ataaagaaga aacgagtcag gaaaatgaca tcgaatggca acatgcgcgg
     6061 gcctgaggcc aaagaaatgt aaaacactgt tgttatttgt aacaacggaa acatttacgg
     6121 aaattatttg tctttcttcg tgatcaaaaa tgctgtgtct tgttggattg gagtgatgag
     6181 ggctgcgttt aggaaacgcc ctatattgta cggttgcatt atcgcggtag gggtaatgca
     6241 cgtgtcagta tgatgagctg accacactca cgtgggtgga ctggtataac aatcgacgat
     6301 tgctggaaag gcagggccat atcccaccgg cagaagcaga aaaagcttat tatgcttcca
     6361 tcggaaacga tgatctggca gcctgagttc acagataaaa cactctccag gaaacccggg
     6421 gcggttcact tagccgcgtg tccattattg ctggataaga tcataacgat acaccgggtt
     6481 aaacaggtgg tcactgcttc actgaagctg cgtgattatg atggtcaggt agcagaagct
     6541 atgcccatga taccaaaatg acgaaagccg aaatgtctga aagcgtgtgt attacctgaa
     6601 aatatgaccg gctccggggt acgtaaccga ttcaacaaag ccaatcgata ttacaaaacg
     6661 agagggacta tatatttcgt tttcagataa gactccttga gagattatct tttatgtaaa
     6721 atctcaaata tcacctgatg taatgtcaat gataatatta agtgcgctct gaacaacatc
     6781 tttcttggtg tgattaacat ctgacaaaaa ga
//
LOCUS       DAZFRE010000176         6748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFRE010000176 DAZFRE010000000
VERSION     DAZFRE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.178"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..360)
                     /locus_tag="ACHKUI_004826"
     CDS             complement(<1..360)
                     /locus_tag="ACHKUI_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600845.1"
                     /translation="MITYAGYKVKIIFIIFLFLVCHVVAYAEMGRDREFCYPGTPENN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGADIFIPVT"
     gene            complement(375..1154)
                     /locus_tag="ACHKUI_004827"
     CDS             complement(375..1154)
                     /locus_tag="ACHKUI_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6600846.1"
                     /translation="MRQYRLWLAMIKVTIVLFLLIFPTAQAGLVIGGTRFAYPENKSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWVRKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     SQGVCEITWQTLDIYNNVLPAWKVNMNPLQMDVLGSQVKTD"
     gene            complement(1120..1692)
                     /locus_tag="ACHKUI_004828"
     CDS             complement(1120..1692)
                     /locus_tag="ACHKUI_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600847.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTSSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTANMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEEGSVEGLALTIFDKNKGSISNDVKSIGFSLTPSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFMVSYD"
     gene            complement(1643..2206)
                     /locus_tag="ACHKUI_004829"
     CDS             complement(1643..2206)
                     /locus_tag="ACHKUI_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600848.1"
                     /translation="MNLIFSKYSVFFIFFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRISAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(2206..4800)
                     /locus_tag="ACHKUI_004830"
                     /pseudo
     CDS             complement(2206..4800)
                     /locus_tag="ACHKUI_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(4837..5589)
                     /locus_tag="ACHKUI_004831"
     CDS             complement(4837..5589)
                     /locus_tag="ACHKUI_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6600849.1"
                     /translation="MKCIIGNKKWVCGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQQGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDI
                     TVSSPVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(5690..6394)
                     /locus_tag="ACHKUI_004832"
     CDS             complement(5690..6394)
                     /locus_tag="ACHKUI_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6600850.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         2058 a         1468 c         1214 g         2008 t
ORIGIN      
        1 cgtaacagga ataaatatat ccgctccctg tggattacct gaagcacctg aaggatctat
       61 tcctgaaata cctgaaaaat aaacttcata atcatcaaga gaattacagt atgctttata
      121 tcgacctatt ttccagtttt gagatggtaa aacagtacca gctgaattgt tgttaacatc
      181 tgaaataata actgtaccaa aatcataaaa aaaggtcgct ggggtagtat tgttttctgg
      241 agtcccagga tagcaaaatt cacgatcccg gcccatttca gcataggcta cgacatggca
      301 aactaagaat aaaaaaatta taaaaataat tttcacttta tatccggcat aagtaatcat
      361 atgatttcct tttactaatc cgttttaact tgtgagccta acacatccat ctgcaacgga
      421 ttcatattta ctttccatgc aggcaataca ttattataaa tgtccaatgt ctgccatgtt
      481 atttcacaaa ccccttgcga tttacaccat gattgttggc ggtggctaaa tggtgcgatc
      541 ataccaccat ccataacttt accatcaaca tgaaggttat agagtgtcac atacctgggg
      601 cctggattat aaacggtaac aatttcattt tttcgtaccc agcgcaattt ttgatatgac
      661 atattggggt cttcagacaa atttgatggc cgatagaata ttttcatacg attgcggacc
      721 gctatttgca attgattatc agttttggtt ggatgactat tttccaacga tggtaatgca
      781 gcaacattaa gtataaataa actttcccgg tcggcaggta aactaccacc gacacgatta
      841 attcttattt ttgtattttt gtcaggcttc aaaacaaaca gtggcggagt tgcgataaat
      901 accgttccag aaaatgaggg caaagattca accgttcccg tcaactgacg agcatcatca
      961 ggggagactg caaccttgac taacatatct ctgtctgaag tgtttttgag ttcaacagat
     1021 attgaggact tattttccgg atacgcaaaa cgcgttccac cgattactaa tcctgcttgt
     1081 gcagtaggaa atatcaggag gaataatact atggtaacct taatcatagc taaccataaa
     1141 cgatactgac gcatttgcat cccctggttg tacattattt tttaatgctt tgtaagccgc
     1201 aaaaaaatgc aatatattat caaccgatgg tgtaagtgaa aaaccgatgc ttttaacatc
     1261 attactaata ctacctttat tcttatcaaa aatcgtgagt gcaagcccct ctaccgagcc
     1321 ttcttccagc ttatataaac tggcatcttc tgaaactgta ttaccggtaa actttatata
     1381 cgcgttgcca acatcactac cacagcgggt taacgttatg gaaaaataaa ccttttcagt
     1441 aacgtctcct gcactcctta ccgaactact gggtacgtct ggcatattag ctgtaaaatt
     1501 ttttgactca tcagaaataa tgcaggtttt ttcggtaatt aacccttcaa tacgaaccac
     1561 tccggcatga gtgatatcat cagcaaaaga acttgttgat attataaaca aaaataaact
     1621 aatgaatatc ttcatatctc cctcactgat aatcgatatc aaaatacaac agagcatttg
     1681 cttgcccggc atgtacattt tcctgagttg atttatagcg aagtttaaac gtcaaattag
     1741 catcaccgtt ttcatttaac actaaatcac gaaaacctga ttcaccatta atggcaatcg
     1801 ccacatcatt gttatccaat atttctattc ccagtccagt cgcactatct gcaccggcgg
     1861 agatacgcag taatgcgcta ttaatagcat ctgcatcacc agtaaaccgc acattggcgc
     1921 tcacaccaga actataaaca gtactttttg cagtcgggca tccttgtaca ttaatcgtaa
     1981 acgtttgact cggagaaaca tcacctacat ttaaaaattc attagcatca taagtgccca
     2041 tttttaccga ctgatcttta cttccggtag taatgtcaca gctttttgcc aacaaaatcc
     2101 cagtaaatgt aacaacaact gaatcattgg cctgccctga gagcggaaaa gcaaaaaaag
     2161 cgaaaaaaat aaaaaataca gaatatttag agaatattaa attcattagc ggcactccag
     2221 agttgcatag ctaacaggat tatccagttc tgtttcaggg agcttgtaat tagcctgaca
     2281 tttttgctga acaccgtctc cccactggat attaataaca ccttctaaag gcaatcctga
     2341 gagataaagt tgtccattat cgccgacaat cccggtaatt tgactactgt ttttaatctg
     2401 agcgctggct gtagcaccga atgggactga tttattacga taacgaactg tgaacaaacc
     2461 acgaataccg atatgagcag caaactctgc acgaactaac gcaccttttc ccggaataac
     2521 ttcagtggaa gtgatatcca gttcagtatg catattcatt gacataggat ctaatgaaat
     2581 attattacgt ctatattctg ttgcataagg aacaactgca tatccacgcc agtcagtgga
     2641 tattcctgtc ccccgctgaa catccacatt attcgctccg ggcgctttta tcaaaacatt
     2701 agtttcacct aatggttgac tcaacgtaag accattttca tgtacaacaa gagcaccact
     2761 gatgccatag tttatttgtt gattaccttc attatagtaa ctgtaggcgg aattcaagga
     2821 accatatgcc ccacgataat taacaccaac agaaccacta ctgctattat cctgattagc
     2881 aaaaccatgt tgtatgttat aactgagatt atgctgctcc aacagagaac cagatacccc
     2941 cgtatattgg tttacctgtc cgtggttatc ctgactataa ccatacgttg cagtcatggc
     3001 attcgatgat gagcgcccat cattcgtgga agataaccaa ttacttatag gtaatgagac
     3061 atttagcgag acaattttat ccgcatttgt ccctggtgat ttactgtaat tccacacaac
     3121 atttagatag atacccttaa tactggtgtt ataaccaact tggtataaag aatctttttt
     3181 atcagtattc cagtatgttt gctgactagc cgataaatac atagacccgt agttaccgaa
     3241 aggttgcgat acactagcct gaaattttcc acgtttgcta taacgcaagt tataaaaatc
     3301 attccagtta taaacgtaat ctttatcatc taacattttt ggatcaacaa cagtccctga
     3361 catttgttgg tatgtcgtat cacttaaagt gtaaaagccc taggttgaat aacgatagcc
     3421 aataatatgg aatgctgttc ctgtctgaac taacgatttg gaataaagaa aacgataaga
     3481 ttgcccttga tgtttgctcc catccacaag cgaactgtta gcctgagtaa catcaaacga
     3541 tgttgcacca aatactccca gattcaggcc aaggccgagc gccaacgccc ggtaatcctc
     3601 agagaattgt gccccaccat aagccgtaat atcccagggt aaaccccaaa ttaattcccc
     3661 ctgaaatata taaggacttt tctggttata gctattgctt cgatattggc caaccgtagt
     3721 agaatatttt gaatgacctt ctcgttgcag gatggggaca gcggcataag gtacggtata
     3781 gacagtttct gaattatcag actctttgat ggtaacttcc agatctcccg cagatgatgt
     3841 cgggttaaga tcgctaatct caaacggtcc cggcggcata taggtttgat aaatgacata
     3901 accattctgt ttgattgtta cctgagcgcg acttttcgca attccgcgca ccgtgggagc
     3961 gaaaccactt tgactatcgg gcaacatatt ttcgtcagat tccagtttta tgccccgaat
     4021 actaacactg tcgaaaacat ctgatgatga ccatgtatct cctagcgtta attcacttct
     4081 gaaaggaata ataacgcgct gcaacgtact actgatatgc tccagtttac ctgctgaatt
     4141 actcccacga ctccaggttg aataatcgcg aaaacgccat gcaccaatat tgatcccagc
     4201 gcgaagattt aaataatagt tatttgaaga gtaatcacca cgatcgttat taccagaaaa
     4261 gtcataattc agcaaaaaag ctgtaatacc gttatcccac tgttctggtg gaatatatcc
     4321 gcgggcgtca cgttttatta tcgcctgcgg aaaggtcagt ttaagtctct gtgcatccag
     4381 ctccggcatg taatgcacat caggaaacca gatacgcaaa tcaaggcaac tattttcaga
     4441 tgaagccaaa gcctgctctg gcagcgcatt tttgttaatt ccaaggctta ataagtcagc
     4501 aagtgaaagg cagggaatca gttgattcgc atcgtctgct ttaaaagtta cagtacgaga
     4561 gagtaaaggg gaatcattga cccaaacttc gacttcatag ctaccaggaa gataagtcat
     4621 ccctttctcg aagcgggata agtcagcaac agcttcagta tcacccgaca gaaaagcagg
     4681 gttaaagcgc attcccgcga ttgaacatgc aggcataata accagaagaa gcggcacaac
     4741 ccgtttcatc tgcttttgtg ccgccatcca atattcgcat cttttgagct ttaggttcat
     4801 atccctgacc tgtgttgcat ctgaactatt ccgttgttac tgcattattc ctttctgccg
     4861 ctccgtcgta gcgccaaagt cattaattgt ttgataagta atttcaccag ccaacgtcac
     4921 tggcgacgag accgtaatat cacttttcgg tggtacaaac atattgggca atttactccc
     4981 ttgtacttta attcctgtca gtgtcaggta ataaggcgaa ggattgataa cttttagttg
     5041 ttttccctca tacttaaacc gcaacatatt tcgtgctttt ccttgttgca caggtagatt
     5101 ttccgggcga taaaatagct tgattttgtt ttgtaatgcc aactgtaagg tattcttctg
     5161 tgtatttttc tcatctgttg gcggaattgc tttaacattc atccaataca aagtttcacg
     5221 atcggtcggc aaattagtct taccaagaaa catgatacgt aatgaagctt ctttacgcgt
     5281 tggcaacata aagaggggag gagtgataac aaaatcctgg ctcttttttt ccgttttact
     5341 gtcatcaacc catgattgaa ttaaataact ggtgttgtca ccgctatttt gcacactgag
     5401 tgttatttgc ttactttctg ccggataaat caatcgcgtt acgccaaggc caaacccgcc
     5461 cgcggacgca ttattaataa ataatatcga tagtaaaaaa agaatccctg agcgtataat
     5521 attttgtgat acttttaatt catccctgaa tccacaaacc cattttttat ttccaataat
     5581 acatttcata cagcatcctt gttgatgaaa taacatctgt tattcatact ctattaaata
     5641 atttcaatct caggttacaa ctttttgtaa cctgaaatcg tatttatatt tactcgtaca
     5701 gcacaacaaa acttgctgtt gacttaacta cacctggtgc aacagtgcta ccgttttgag
     5761 cataacctgc agtaaaggcc gcgctgaaat catgggtcgt agcatttcct ggaattgtat
     5821 aagcggcggt ggaggcgcta ccctctgccg cagtggtatt caggttaatc agaccattat
     5881 tcgtcgtacc tttggagtaa attcgaatcc ctacaccagt cgcccccgca ttgccgctgt
     5941 tgtcagcatc cgttgatcct tctacttcgt tagcgagcgt tttattagcg ctatctgcag
     6001 taccggtaaa acggaactca attttctgat catctgtatt gctacattcg gtcagattaa
     6061 tattgaaatc ggtctggttg ctcgccccct gtgcttctac gtcgatacca gtgaaaaggt
     6121 tggaaggcac aaccggcagg acaacttcaa tctgttttga atcggttgcg accttacagg
     6181 tagctttcac tacacggcca gtaaagcgga ttttcccccc cattgaatca acgttgtcat
     6241 ccatatcagt gagcctggga taatcaacgg tgctccagtc agtatccgcc ccaccaaatt
     6301 gtgttgcatc gccgggtgct gcggttaccg cagtagacat cagcatacct gatgccagta
     6361 atactgcgga taaattagat aaattgaatt tcatttatac ctttcctttt gttaaaaaat
     6421 ccaagcaaca ttggtgtttt tacattctgc gttaataagt tatatctcac aaaaaaaata
     6481 agctgacgtt ggctaattcc agaaggttct ttagaagagt aattgagtga ataatattat
     6541 atatgctgct tagtaatgta cctaaaataa tccaaagccg caataagtga gtttatactc
     6601 acatataaca cactgataat gataaaattt atttttacgt ggagttgtaa gaggggcaat
     6661 aaaacataaa tcacatgaat aatattaatg cattcataat aaattaatat tatttgaaat
     6721 aacaaaatac caaaaacatt atcatcaa
//
LOCUS       DAZFRE010000177         6718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFRE010000177 DAZFRE010000000
VERSION     DAZFRE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.179"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(307..2340)
                     /gene="betT"
                     /locus_tag="ACHKUI_004833"
     CDS             complement(307..2340)
                     /gene="betT"
                     /locus_tag="ACHKUI_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6600851.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDS"
     gene            2469..3056
                     /gene="betI"
                     /locus_tag="ACHKUI_004834"
     CDS             2469..3056
                     /gene="betI"
                     /locus_tag="ACHKUI_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6600852.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            3070..4542
                     /gene="betB"
                     /locus_tag="ACHKUI_004835"
     CDS             3070..4542
                     /gene="betB"
                     /locus_tag="ACHKUI_004835"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6600853.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPHTNFGPLVSFPHR
                     DNVLRYIAKGIEEGARVLCGGDVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4556..6244
                     /gene="betA"
                     /locus_tag="ACHKUI_004836"
     CDS             4556..6244
                     /gene="betA"
                     /locus_tag="ACHKUI_004836"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6600854.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIFFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1668 a         1828 c         1791 g         1431 t
ORIGIN      
        1 gacaattaaa ccttaaacaa caaacggtta cattgatttt agttgatttg cgctacgcgt
       61 tccccatgaa taaagcgtca tttcaaatta gcaccaatat ttacattaaa taagactaag
      121 cttattgaac attcacaaca gtccaaacgt aggttttggg cttcaatagt tcttttcttt
      181 tttattatct gttgcctggc aatcatcaat cgatactaag ggcttaactg tacacagata
      241 taacgtatca agaggtaaga gaaaccgcgc catatgcaga catggcgcgg ttttatgcaa
      301 taacaatcac gagtccggga acatcacgct atggcccggc gcttcacgat ggagatggat
      361 aaagttaagg tgccgctcgt actggtcgag aatatcggtg atcacctgct ctttgctgta
      421 gtccatcagg tcgttgccct ggctgccttc taacaggaag gtttccagcc ggtagtaggt
      481 cgatttcccg ctacgtgcgc ggtaggtaaa gcccggcact gaatattgct gcggccaaat
      541 ctgatagaca aagttctgct cttcgcccat atgcaccaac aaatccagat gccccaactg
      601 ttgtccctct tccggcggca ggttttttag ctccacgtac gcgccgcgca accgcagctc
      661 ttgcgccact tcttccattg ccgggtaaca gaccgtctcc atcatctgtt tagtgtaacg
      721 cgtgcccgga taattcatca ggcgcgagag acgttttttc cagctcaggc gatcctgaag
      781 ccccagcggt cgcggcgcgg tatcacggtt ggcactttca cggcggtaat cttctacctt
      841 cagagattta tacaaccccg ccatcacgaa gaaaatcaca aagctgaacg gcagccccat
      901 gattaccgtg gtgttttgca gcgcggatat cccgttggtc atcagcatgc caagcgtcag
      961 cagaccaatc gccaccgacc agaagacgcg cagccagccg ggggcgtcgc tgttgatatc
     1021 tttaagctgc gaggtgaaat tccccagcac cagcgccccg gagtccgccg aggtcacata
     1081 aaacagcagg ccagtaatgg tggcgacgga ggcgctaaag gtaaacgccg gatactgcgc
     1141 cagcaggctg tagaagccgc gctccggatg gaccatcgct tcctcggcaa atgccgcgcc
     1201 gccgtggatg atttcataca gcgcgctatt gccgaacacc gagagccata acagcgtgaa
     1261 ggtaaacgga ataatcaacg tgcccagcac gaactggcga atggtacgcc cacgcgagat
     1321 acgcgccagg aacaagccga caaacggcga ccatgccacc caccatgccc agaagaagag
     1381 cgtccagtta ttcatccact caaccggacg gtcgaaggca aaactgttga gcgtcatgcc
     1441 cataaagcga ttcacatagt cgccaacatt cagcaccagt gcattaagca ggaacgaggt
     1501 gtcgcccata aacaacacga acagaatcaa tcccagcgcc agcgcaacgt tgagttccga
     1561 taacacgcga atgcccttat caacaccgga tgtcaccgag atagtggcga tgatcaccga
     1621 caaggcgatc agtgccgctt tcgccgccat cgaatcggga atatcaaaca gcacgctcaa
     1681 gccatagtta agctgtacca caccgatacc gagcgtagtg gcaataccga agatggtgcc
     1741 gatcaccgca gcaatatcca ctgagtgacc tatcggcccg ttaatccgtt taccgaagat
     1801 cgggtacagc gccgagcgga tagtgagcgg caaattataa cgatagctaa agtatccgag
     1861 cgccatgccc atcagcgcat acatcgacca gccggttaag ccgtagtgaa acagcgtcca
     1921 gaccatcgcc tgacgcgcgg cctcaatcgt ctgtcccgcg ccttccggcg gctgcatata
     1981 ctgcgttacc ggttcggcta cggagaagaa catcaggtcg ataccgatcc cggcagcaaa
     2041 cagcatcgcc gcccaactca gcagactgaa ttccggtttt gattgttctg gcccgagctt
     2101 caccgaacca aaacgcgaac aagcgataca gaccacaaag acaatataga gcgttgccgc
     2161 cagcagatag taccaaccga aggttttaga aacccagtcc agcgtgcggc caatccacag
     2221 ggccgagaag tcgcgaaaca ggatcgttgt cagggaaaac aacaaaatca gtccggcgga
     2281 agtgtaaaac accaccggat tgattttgtc cttttccctg ctgtgtgaaa ggtctgtcat
     2341 ccagtatccc cactgttatt gttaccattt aaaatcaaat tagtaacaat taagacacat
     2401 tttatattga acgtccaatc aataaccgct ttaatagata aacaacgctg atgaatggag
     2461 tggcgaaaat gcccaaattg ggaatgcagt cgatccggcg cagacaactg atcgacgcca
     2521 cgctggaagc aataaatgaa gtgggcatgc acgatgcaac gatcgcgcag atcgcccgcc
     2581 gtgcaggcgt ttctacgggg atcatcagcc actatttcag ggacaaaaat ggtctgctgg
     2641 aagcaaccat gcgcgatatc accagtcagc tgcgtgacgc ggttttgaat cgattacatg
     2701 cacttccgca gggcagtgca gagcggcgat tacaggcgat tgttggcgga aacttcgatg
     2761 aaacccaggt gagcagtgcg gcgatgaaag cctggctggc cttctgggcc agcagtatgc
     2821 atcagccgat gctctatcgc ttgcagcagg tcagtagccg ccgcttattg tcgaatctgg
     2881 tgagcgaatt tcgccgcgag ttaccgcgcc aacaggcaca ggaagcgggc tacggccttg
     2941 ccgcgctaat tgacggatta tggctgcgcg cggcactgag cggcaaagcg ctggataaac
     3001 ctctcgcaca ctcgctgacc cgtcacttta ttactcagca tttacccacc gattaaccga
     3061 ggaaacgtga tgtcccgaat ggcagaacag cagctttata tacatggtgg ttatacctcc
     3121 gccaccagcg gtcgcacctt cgagaccatt aacccggcca acggtaacgt gctggcgacc
     3181 gtgcaggccg ccgggcgtga ggatgtcgat cgcgccgtga agagtgccca acaagggcaa
     3241 aaaatctggg cggcgatgac cgctatggaa cgctcgcgta ttctgcgtcg ggccgttgat
     3301 attctgcgtg aacgcaatga cgaactcgca aaactggaaa ccctcgacac cggaaaagca
     3361 tattcggaaa cctcaaccgt cgatatcgtt accggtgcgg acgtgctgga gtactatgcc
     3421 gggctgatcc cggcgctgga aggctgccag atcccgttgc gtgagacgtc atttgtttat
     3481 acccgccgcg aaccgctggg tgtggtggcg gggattggcg catggaacta cccgatccag
     3541 attgctttgt ggaaatccgc ccccgcgctg gcggcgggta acgcgatgat cttcaaaccg
     3601 agcgaagtca ccccgcttac cgcgttaaag ctggctgaaa tttacagcga agcgggcctg
     3661 ccggacggtg tatttaacgt gttgccgggc gtgggcgcgg agaccgggca atatctgacc
     3721 gatcatccgg gcattgccaa agtgtcattt accggcggcg tcgccagcgg caaaaaagtg
     3781 atggctaact cggcagcttc ctccctgaaa gaagtgacca tggagctggg cggtaaatcg
     3841 ccgctgatcg tttttgacga tgcggatctc gatctcgccg ccgatatcgc catgatggcg
     3901 aatttcttca gctccggtca ggtgtgtacc aacggcactc gcgtcttcgt tccgacgaaa
     3961 tgcaaagccg catttgaaca aaaggttctg gcgcgcgttg agcgcattcg cgcgggcgac
     4021 gttttcgatc cgcacactaa cttcggcccg ctggtcagct tcccgcatcg cgataacgtg
     4081 ctgcgctata tcgccaaagg catagaggaa ggcgcgcgcg tactgtgcgg cggcgatgta
     4141 ctgaaggggg atagcttcga taacggcgca tgggttgcac cgaccgtgtt caccgattgc
     4201 agcgacgata tgaccattgt gcgcgaagag attttcgggc cggtgatgtc cattctgacc
     4261 tacgagtcgg aagacgaagt cattcgccgc gctaacgata ccgactacgg tctggcggcg
     4321 ggtatcgtga cggcggacct gaacctcgcg catcgcgtca ttcatcagct ggaagcgggt
     4381 atttgctgga tcaacacctg gggcgaatcc ccggcagaga tgcccgttgg cggctacaaa
     4441 cactccggca ttggtcgcga gaacggcgtg atgacgctcc agagttacac ccaggtgaag
     4501 tccatccagg ttgagatggc taaattccag tccatattct aaccaggagg tttatttgca
     4561 atttgactac atcattattg gtgccggctc agccggcaac gttctcgcta cccgtctgac
     4621 tgaagatccg aacacaaccg tgctgctgct tgaagcaggc ggcccggact atcgctttga
     4681 cttccgcacc cagatgcccg ccgcgctggc gttcccgcta cagggcaaac gctacaactg
     4741 ggcctatgag acggaacccg aaccgtttat gaacaaccgc cgcatggagt gcggacgcgg
     4801 caaaggtctg ggcggttcat cgctgatcaa cggtatgtgc tacatccgtg gcaatgcgct
     4861 ggatctcgat aactgggcac aagaacctgg tctggagaac tggagttatc tcgactgcct
     4921 gccctactat cgcaaggccg agactcgcga tgtgggcgag aacgactacc acggcggtga
     4981 tggcccggtg agcgtcacca cctccaaacc cggcgtcaat ccgctgtttg aagcgatgat
     5041 tgaagcgggc gtgcaggcgg gctacccgcg cacagacgat ctcaacggct atcagcagga
     5101 aggttttggc ccgatggatc gcaccgtcac gccgcatggc cgtcgcgcca gcaccgcgcg
     5161 tggctatctc gatcaggcca aatcgcgccc taacctgacc attcgtactc acgccatgac
     5221 cgatcacatc ttttttgacg gcaaacgcgc ggtgggcgtc gagtggctgg aaggcgacag
     5281 caccattccg acccgcgcgg cggcgaataa agaagtgctg ttatgtgcag gcgcgattgc
     5341 ttcaccgcag atcctgcaac gctccggcgt cggcaacgct gaactgctgg cggagtttga
     5401 tattccgctg gtgcatgaat tacctggcgt cggcgaaaat cttcaggatc acctggagat
     5461 gtatctgcaa tatgagtgca aagaaccggt ttccctctac cctgccctgc agtggtggaa
     5521 tcagccgaaa atcggtgcgg agtggctgtt tggcggcacc ggcgttggtg ccagcaacca
     5581 ctttgaagca ggcggattta ttcgcagccg tgaggaattt gcgtggccga atattcagta
     5641 ccatttcctg ccagtggcga ttaactataa cggctcgaac gcagtgaaag agcacggctt
     5701 ccagtgccac gtcggatcga tgcgctcgcc aagccgtggg catgtgcgga ttaaatcccg
     5761 cgacccgcac cagcatccgg cgattctgtt taactacatg tcgcacgagc aggactggca
     5821 ggagttccgc gacgcaattc gcatcacccg ggagatcatg catcaaccgg cgctggatca
     5881 gtatcgtggc cgcgaaatca gccccggtgt cgaatgccag acggatgagc agctcgatga
     5941 gttcgtgcgt aaccacgccg aaaccgcctt ccacccgtgc ggtacctgca aaatgggtta
     6001 tgacgagatg gcagtggttg acgccgaagg ccgcgtacac gggctggaag gcctgcgcgt
     6061 ggtggatgcg tcaattatgc cgcagattat caccggcaat ttgaacgcca cgacgattat
     6121 gattggcgag aaaatggcgg atatgattcg cgggaaggat gcgttgccga ggagcacggc
     6181 aagatatttt gtggcaaatg ggatgccggt gagagcgaaa aaaatgagtc gtgatgtgaa
     6241 ctgactcagg aacctatcag attgagagtt accgttctac tatgaggacc gaatccttat
     6301 ataaaacact gaggtaactc tcatgcttca tcgtgacaat ctcaccagca aattctaagc
     6361 tgacttgctc aatctggctg atgaaggtgc tatgcataca cagataaact acagatgaaa
     6421 taccactcag cttaagaatt acgccgctaa tgttcctggc tatggttacc tgttcattcc
     6481 cgctcacggt tagcatgtac gaaaagggtt cttgtaacga gtacaaaaat aagtcagacc
     6541 aaaaccacat ggagctttaa acacggttcg tataatttat tagcagcgat ttatttttac
     6601 tcttctttaa ttaatcatca tgcttaaata ttttttagca caagattatc gcttcgccaa
     6661 cattttattt tacattcatc atcaattgat aataaaaaat tgttaataat tttttgca
//
LOCUS       DAZFRE010000178         6562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFRE010000178 DAZFRE010000000
VERSION     DAZFRE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.180"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            62..838
                     /gene="exuR"
                     /locus_tag="ACHKUI_004837"
     CDS             62..838
                     /gene="exuR"
                     /locus_tag="ACHKUI_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6600855.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1078..1161
                     /locus_tag="ACHKUI_004838"
     CDS             1078..1161
                     /locus_tag="ACHKUI_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600856.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHKUI_004839"
     CDS             1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHKUI_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6600857.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHKUI_004840"
     CDS             1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHKUI_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6600858.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHKUI_004841"
     CDS             2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHKUI_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6600859.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            2785..3090
                     /gene="yqjD"
                     /locus_tag="ACHKUI_004842"
     CDS             2785..3090
                     /gene="yqjD"
                     /locus_tag="ACHKUI_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6600860.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /gene="yqjE"
                     /locus_tag="ACHKUI_004843"
     CDS             3093..3497
                     /gene="yqjE"
                     /locus_tag="ACHKUI_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6600861.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /gene="yqjK"
                     /locus_tag="ACHKUI_004844"
     CDS             3487..3786
                     /gene="yqjK"
                     /locus_tag="ACHKUI_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6600862.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4364
                     /gene="yqjF"
                     /locus_tag="ACHKUI_004845"
     CDS             3972..4364
                     /gene="yqjF"
                     /locus_tag="ACHKUI_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6600863.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            4434..5420
                     /gene="yqjG"
                     /locus_tag="ACHKUI_004846"
     CDS             4434..5420
                     /gene="yqjG"
                     /locus_tag="ACHKUI_004846"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6600864.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5703..6071
                     /gene="yhaH"
                     /locus_tag="ACHKUI_004847"
     CDS             5703..6071
                     /gene="yhaH"
                     /locus_tag="ACHKUI_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6600865.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6105..>6562)
                     /gene="yhaJ"
                     /locus_tag="ACHKUI_004848"
     CDS             complement(6105..>6562)
                     /gene="yhaJ"
                     /locus_tag="ACHKUI_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6600866.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT         1637 a         1639 c         1658 g         1628 t
ORIGIN      
        1 ggcatacgca tgcataaagt ggtatgataa ctttgtccgc attccactcc tggagacgca
       61 tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc tgaaagagcg
      121 catcgaacag ggcgtctatc tggtgggtga taaactgcct gccgaacgct ttattgccga
      181 tgaaaaaaac gtcagccgca ccgtggttcg tgaagccatc atcatgctgg aagttgaagg
      241 ctatgtcgaa gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc cgcgccatca
      301 gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt tgctccaggc
      361 tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtca caaaacagga
      421 catcatgaaa ttgatggcca ttcaggagca ggcacgcggt gaacaatgct tccgtgattc
      481 cgagtgggat ttgcagttcc atattcaggt agccctggcg acgcagaact ccgccctggc
      541 ggctatcgtt gaaaaaatgt ggacccagcg tagtcataac ccgtactgga aaaaactgca
      601 cgaacacatt gatgcccgta ccgtcgataa ctggtgtgat gaccacgatc aaatcctcaa
      661 ggcgctgatt cgcaaagatc ctcatgctgc taagctggca atgtggcagc acctggaaaa
      721 caccaagatc atgttattta acgaaaccag cgacgatttc gagttcaatg ccgaccgcta
      781 tctgttcgcc gaaaacccgg tggtgcatct cgatacagcc accagcggca gtaaatgaaa
      841 ttcctgtccg acaggcgcta cagtgcgcct gtttgtctcg tcggtaagcg aaaagtataa
      901 agtgtcagcc tgtgtaaatc ctctcgcctc cctcccctgc attcagcaaa atcagactcc
      961 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
     1021 aatttatgct tttgaatgct ttcttatctc acgatttaac agggaatagt tcaggctgtg
     1081 ttgatgtatc aaacccgcag aacataccaa gacagcaata acattgcggt agtgcatcat
     1141 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
     1201 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccgtc gatgattggc
     1261 atgttgtatt ttgtcttgtt tgtcattttg ttccttgaaa acggcttgct tccggcggcc
     1321 tttttaccgg gcgacagttt actggtactg gtcggcgtat tgattgcgaa aggcgcgatg
     1381 ggctatccgc aaacgattct gctgctgacc gttgccgcca gcctcggttg ctgggtcagc
     1441 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1501 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1561 ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc cgggttatca
     1621 gggctgaata acgcgcgctt tcagttcttc aactggatga gtggtctgct gtgggtgttg
     1681 atcctgacaa ctctgggtta catgctcggc aaaacgccgg tatttttaaa gtacgaggac
     1741 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1801 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1861 cgcatgtcac ttcgccagct agcctggtcc ggcgctgttt tacttctggt cagcacacta
     1921 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     1981 caagggacaa cgatgccaga cggtttttca atctggcatc accttgacgc tcacggcatt
     2041 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacctttga ctccagcgac
     2101 cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta catcattgcg
     2161 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttgcggga taactctcat
     2221 cgcttcggat aacttcctgt tatccgaaac agatcactca ctttggtgat ttcaccgtaa
     2281 ctgtctatga ttaatgaggc ggtggttaac acgaccgctt cgttgtctgg acaatcgttc
     2341 ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc atcgctttag
     2401 ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt caggaaaagg
     2461 agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag aatcgtattg
     2521 acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc caactgcgtg
     2581 ccgatcatca gaagaaaatc gcaaagcaga aagatgaggt ggcggaacgc cagcaagatt
     2641 tagccgaggc gaagcaaaaa ggcgatgcag ataagattgc caaacgcgaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctga aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtca
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccattgcc aaacaaaccc gtgtcgcggc ggcgcgtgcc
     3001 gatgagtatg tgcgtgaaaa tccgtggacg ggcgtgggca ttggcgcagc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 agacacgtct gcggctggcg gtggtggagc tggaagagga aaaagcgaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctagttat ctgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gcatctggac gttacgtaaa tcgcgtaagt
     3421 ctacgttgct gcgccacacg cgccatgagc tggcaaacga tcggcagctg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatct ttccgccact cgtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgttgagt ctgcgctcct gggcgctggt tggcagtagc
     3661 gtgatggcta tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggtgtat ggagcgcctg gcgtctggtg aaaacaaccc tcaagcaaca acagcttcgc
     3781 ggttaacttc ctctctggcc ggagaaattc cggccttatc cctcaaattt tttgaaaata
     3841 tttgacagtt ttccttgcta acaatcacgc ttcgtcacgt ttatgattct ctccatcgac
     3901 agcaacgacg ctaatagcgc gcccttgcac aaaaaacaat cagcagcctg agcggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgt attttaatgc
     4021 cgattctgtt tattacggca ggctggggaa aaattactgg ctacgcgggt acccaacaat
     4081 atatggaagc aatgggcgtc cccggcttta tgctgccgct ggtgattctg cttgagtttg
     4141 gtggtggtct ggcaatcctg ttcggtttcc tgacacgtac cacagccctg tttactgcgg
     4201 gctttacgct gctgacggca tttttatttc acagcaactt tgcagaaggc atgaactcgc
     4261 tgatgtttat gaaaaacctg actatttctg gcggattcct gctgctggca attaccggtc
     4321 caggcgcatt cagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta
     4381 acgataaaaa acgaggggga agctcctcgt ttttgctatt ggaggataga aaaatgggtc
     4441 aactgattga cggcgtctgg catgacacct ggtacgatac caaatccacc ggcggtaaat
     4501 ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgcg ccaggcccca
     4561 ctggcaaagg cggttttgcc gcagagaaag atcgttatca tctctatgtt tcactcgcct
     4621 gcccgtgggc acaccgcacg ctgatcatgc gtaagctcaa aggactggaa ccgtttattt
     4681 ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc
     4741 cgggagcaac cggcgacacg ctctatcaac atgaatttct gtatcagctt tatctccacg
     4801 ccgatccaca ctacagcgga cgagtgactg ttcccgtgct gtgggacaaa aaaaaccaca
     4861 ccatcgtcag caacgaatca gcagaaatca tacgcatgtt caataccgcg tttgatgcgc
     4921 tgggtgcgaa agctggagat tactacccac cagccctgca accgaaaatt gacgaactta
     4981 acggctggat ttacgacacc gttaacaatg gcgtgtataa agccggtttt gccaccagcc
     5041 agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga ctggaacaga
     5101 ttttaggtca gcaccgttac ctgaccggca accagctaac cgaagccgat attcgcctgt
     5161 ggaccacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc
     5221 gcatcagcga ttacctgaat ctgtatggtt tcctgcgcga tatctaccag atgccgggaa
     5281 ttgccgaaac agtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca
     5341 tcaaccctac gggaattatt tcaattggtc cgtggcagga tctcgatgaa ccgcatggac
     5401 gagatgttcg cttcggttaa aaaaaggcac cttacgaggt gcctctgata attagctaag
     5461 tacgtgttgt tcatatcgga tgcggcgtaa acgccttatc aaacctacaa aacatttata
     5521 ttcaatatgt tgcaatgacc gcgcaggcca gataagcgta gcgcatctgg caattttgcg
     5581 cttgccctta tccttcacag cgccttatta attcatcgcg ataatattta ctgccatttc
     5641 attcacgtct attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa
     5701 aaatggactg gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc
     5761 gcaaagagta ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctac
     5821 tggataaaat gttaggctgg caacgtgccg gtggcgaagg catcctgacg acaatctacg
     5881 gtattctggt gtttttacca tggtgggcag ttcagttccg ccgcctgcac gacaccgacc
     5941 gctcggcgtg gtgggcactg ctattcttaa tcccgttcat cggctggctt attatcatcg
     6001 tctttaactg ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccgaaactgg
     6061 aacaagaata ataattaagc accttgtaaa aggtgcttaa tcgattattt tccgctaaaa
     6121 agtttgggaa tttcccgcag acaccaggat ttcgcctccc ccatactgtc acgacgccag
     6181 gccataataa tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc
     6241 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
     6301 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcaactgt
     6361 acggtcaaca ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcacg
     6421 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatccgg cgcggcaaca
     6481 tagacgttca ttaaggtata gagtttgcgc gagttgatct ccgacgagga acgaaaatgc
     6541 atatccggcg cgataacgat at
//
LOCUS       DAZFRE010000179         6427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFRE010000179 DAZFRE010000000
VERSION     DAZFRE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.181"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..300
                     /locus_tag="ACHKUI_004849"
     CDS             <1..300
                     /locus_tag="ACHKUI_004849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600867.1"
                     /translation="KVVYGCMQGTGVIFRDTVTVSGCLLLRPLRLPAAWRHPVVRPDS
                     DGQLLAHPPVSAEPAGAVSGCRYGPMRENPGLQRPFRSRRPCRAVTCAPCTPDGV"
     gene            653..1180
                     /locus_tag="ACHKUI_004850"
     CDS             653..1180
                     /locus_tag="ACHKUI_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6600868.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            1238..1471
                     /locus_tag="ACHKUI_004851"
     CDS             1238..1471
                     /locus_tag="ACHKUI_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6600869.1"
                     /translation="MSEYFRILQGLPDGAFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMKHVIRNFGILK"
     gene            1530..3488
                     /locus_tag="ACHKUI_004852"
     CDS             1530..3488
                     /locus_tag="ACHKUI_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6600870.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYCVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDARAYRNLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            3543..3977
                     /gene="psiB"
                     /locus_tag="ACHKUI_004853"
     CDS             3543..3977
                     /gene="psiB"
                     /locus_tag="ACHKUI_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6600871.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWVMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3974..4693
                     /locus_tag="ACHKUI_004854"
     CDS             3974..4693
                     /locus_tag="ACHKUI_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6600872.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            4690..5286
                     /locus_tag="ACHKUI_004855"
     CDS             4690..5286
                     /locus_tag="ACHKUI_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600873.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAVRDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            5748..6248
                     /locus_tag="ACHKUI_004856"
     CDS             5748..6248
                     /locus_tag="ACHKUI_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6600874.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         1319 a         1749 c         2016 g         1343 t
ORIGIN      
        1 aaggtagtgt atggctgtat gcagggaaca ggtgtgatat tccgtgacac tgtcaccgtt
       61 tctggctgtc ttttattgcg cccgcttcgg ctcccggctg cctggcggca tccggtcgtc
      121 aggcccgact ccgacgggca gctgctggcg cacccgccag tctccgccga acccgctggc
      181 gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc cgggcttgca gcgaccgttc
      241 cggtcgcgcc gcccctgtcg cgccgtgacc tgcgctccct gcacacctga cggtgtgtga
      301 ggaaactgcg cctgatggcg cagcagtcct ccctcctgcc gccccccctg cggcctgttt
      361 ccccgctgtg gactttccgg ggggcagacg taaccccctc ccgttccatc tgtccagggt
      421 gagatgcacc ggccacaaaa tttttagcca tgaacccggc aaaaaatttt gcgtccgccc
      481 ccggacagct gccccgtgag gcggtaaacg ggcccccatc ggaaaggtcc actgcgggga
      541 gaaacccctg tgaagtggac ggcaggagga gctttcgctc ctctgaaggg gcacatgaac
      601 gtcccgtcgc cgctatccgg tttatctcac atctttcagt caggaggttc ttatgagtgc
      661 acgtggtatc aacaaggtca tcctcgtcgg gcgtctgggc aatgatccgg aagtccgtta
      721 catccccaac gggggcgcag tggcaaacct gcaggtggct acgtcagaaa gctggcgcga
      781 caaacagacg ggggagatgc gggagcagac ggaatggcac cgcgtggtgc tgttcggcaa
      841 gctcgcggaa gtggctggtg aatatctgcg caagggcgcg caggtctaca tcgaggggca
      901 actccgtacc cgtagctggg aagataacgg tatcacccgt tacgtcactg aaattcttgt
      961 taagaccacg ggcaccatgc agatgctggg gagtgcacca cagcagaacg ctcaggcgca
     1021 accgaagcct cagcagaatg gtcagccaca gagtgctgac gcgacgaaaa aaggtggcgc
     1081 gaaaacgaaa ggccgtggac gtaaggccgc gcagccagag cctcagccgc aaacgccgga
     1141 gggggaggat tacgggtttt cagacgatat cccgttctga ctggcctgac tgtgataacc
     1201 gccccgccct gcgcggggca tcaccggaga gatgaggatg agcgaatatt tcagaatact
     1261 tcaggggctg ccggacggcg cctttacccg cgaacaggcg gaagccgttg ccgcacagta
     1321 ccggaacgtc tttatcgagg atgaccacgg ggaacatttt cgcctggttg tccgcaataa
     1381 cagtggcctg gtctggcgta cctggaattt tgaggacggt gccgggtact ggatgaaaca
     1441 tgtcatccgg aatttcggca ttcttaagta agagaggtgc cggacgcgca aacgtccggc
     1501 aggacataag caattataag gggatgatta tgcctgtaac gaagtgtgaa ccagaaacca
     1561 cccgcaaagc aagccgtaaa tatgcaaaaa cgcaggaaac agtcctgtct gccctgctgg
     1621 cgcagacgga ggaagtgagc gtgccgctgg cctcactgat taaatcaccg ctgaatgtgc
     1681 gcacggtgcc gtattctgcg gagtccgtca gcgaactggc tgattccatt aagggagtcg
     1741 gcctgctgca gaatctggtt gttcatgccc tgccaggtga ccgctactgt gtcgccgcag
     1801 gcggtcgcag actggcagca ctcaacatgc tggcagagcg tggcatcatt ccggctgact
     1861 ggcctgtacg cgtgaaggtt attccgcagg agctggcgac tgccgcatca atgactgaaa
     1921 acggtcatcg tcgggatatg caccctgccg aacagattgc cggattccgt gcgatggcgc
     1981 aggaaggcaa aactcctgca caaatcggtg atttgctggg ttattcgccc cgccacgttc
     2041 agcgaatgct gaaactggca gaccttgcgc ctgtcatcct cgatgcgctg gcagaagacc
     2101 gcatcaccac cgagcactgt caggcgctgg cgctggagaa cgacaccgcg cgtcaggtgc
     2161 aggtgtttga agccgcctgc cagtcgggat ggggcggtaa accggaagta cagaccattc
     2221 gtcgtctggt gaccgaaagt gaagtggcgg tggcggggaa cagtaaattc cgcttcgtgg
     2281 gggctgatgc cttctcgcca gacgaactgc gcaccgattt gttcagcgat gacggggacg
     2341 gttatgtcga ccgcgtggcg ctcgatgccg ccctgctgga aaaactccag gctgtcgcag
     2401 agttccttcg ggaagccgaa ggctgggaat ggtgcgccgg acgcatggag cctgtcggtg
     2461 agtgccgtga agatgcccgg gcataccgca acctgccgga gccggaagcg gtgctgacgg
     2521 aggcggaaga cgaacgtctg aacgaactga tggctcgtta cgactcgctg gaaaaccagt
     2581 gtgaggaatc tgacctgctg gcagcagaaa tgaagctgat tgactgcatg gcgaaggtca
     2641 gggcgtggac gccggagatg cgtgccggaa gtggcgtggt ggtgtcctgg cgttacggta
     2701 atgtgtgtat ccagcgtggt gtgcagttgc gcagtgagga tgatgtggct gacgacgctg
     2761 accgcacgga acaggggctg gagagcgcgt cagtggagga aattagtctg ccgttgctga
     2821 ctaaattgac ctcagagcgc acgctggcag tccaggcagc actgatgcag caaccggaca
     2881 aatccctggc actgctggca tggacgctct gcctgaatgt gtttgacagc aaagcgcata
     2941 gtagtcccgc cagaattcgc ctggaatgtg agcattatgc gctgaccagc gatgcaccgt
     3001 cagggaagga aggtgccgca ttcatggcgc tgatggcaga acactctcgt tttgctgccc
     3061 tgctgccgga aggatgggaa cgggatatga cgacgttcct gtcacttagc caggaggtgc
     3121 tgttatccct gctcagtttc tgtaccgcgt gcagtcttaa cggtgtccag acccgcgagt
     3181 gtggtcacac gtcacgcagt ccgctggact cgctggaaac cgccatcggc tttcacatgc
     3241 gcgactggtg gcagccgaca aaagcaaact tcttcggaca cctgaaaaag ccgcagatta
     3301 tcgcagccct gaatgaggcc ggactgtccg gtgccgcacg ggacgcggag aaaatgaaga
     3361 aaggcgatgc ggctgaacat gcagagttcc atatgaaaga caaccgctgg gtgcctggct
     3421 ggatgtgtgc accacgtcca cagacggatg ccactgaaca caccgctaac ctggctgatg
     3481 ccgcctgatg aacaaccaca ccgccccgcc ggagacgggg cggcagcaag ggagataccg
     3541 tgatgaaaac tgaactgacc ctgaatgccc tgcagtccat gaacgcacag gaatatgaag
     3601 atatccgtgc tgcgggaagc gatatgcgcc gtaatctcac tcacgaggtg atgcgtgaag
     3661 tggacgcacc ggctaactgg gtgatgaacg gagagtatgg cagtgagttc gggggctttt
     3721 tccccgtcca ggttcgtttc acgccagccc acgaacgttt ccacctggca ttatgttcgc
     3781 cgggagacgt ctctcagctc tggatgctgg ttctggtgaa tggtggtgga cagcctttcg
     3841 ccgtcgttca ggtgcaacat atcttcacgc ctgtcgcgat cagtcacacg ctggcgcttg
     3901 ccgcgacact ggatgcgcag ggatacagtg ttaacgacat catccatatc ctgatggcag
     3961 aaggaggtca ggcatgagcg cacgttcacg ggcactgatc cccctcagcg cagaacaaca
     4021 ggccgccatg caggcggtgg ctgtcacaga acaacgtcgt cgtcagggac gcacactttc
     4081 agcatggcct tatgccaccg ctttctttcg ctgcctgaat ggcagtcgcc ggatttcgct
     4141 gaccgatctc cgcttttttg cccctgcgct gacgaaggag gaatttcatg gcaaccgtct
     4201 cctgtggctg gctgccgtgg ataaactgat tgaaagtttt ggggaagtct gtgttcttcc
     4261 cctgccatcc gatgcggggc accgtctgtt cccgtccgtg ccttttcgtg aaggtgagcg
     4321 tcgtcgtcag aaaaccacgc tgacagagca gaaatacagc cgccagcggg aacgtgaggc
     4381 agaacgacgg gaactggaat accagacatg ttttgctcag gcgcagattg accttgcgtt
     4441 tcatactccc gccacggtcg gaagctggtt gtcccgctgg tctggtgttg ttgaggagca
     4501 tgatctggaa acgattttct gggggtggtg cgggcgtttt ccatcactgt catcatttga
     4561 ccggtttttc tggcaggagg aaccactctg gcggctgatt tttgaagccg gtgaggccgg
     4621 tcgtggtgca ccggtacagg tacgtgcact tgagcagtgg atgatcccga acaagctgga
     4681 gaacgtaata tgatgaaatc agacgaaaaa taccaggttc ccgcgtggat gcgacctctg
     4741 ttgccgttgc tctgcaacac cggggggaac gatccggaag aactgctgaa tgatacagaa
     4801 accactgcca gtgcgaatgt tgtccgttat gtactgatag ttgctgtgcg gtcgcaggtt
     4861 gatctgctgc agcttctgta caggaaaggg ctgttgcgca cagagatacc aggtggcttt
     4921 tcaccggaag aagcgcaggc actcctggat aatctggtgc gcagccatat cagcaaggcg
     4981 ctgtcgggcg agcgaatggc agtccgtgac agaaatgccg atctggcctg gattggacag
     5041 caactggtcg atgccgcctg gtttgtccgt gccacactgg aagcgcatgg gatgagcgtc
     5101 ggaaatgaga gtccccctgc tccgccggag acaatgccgg acatacagac acgggaactg
     5161 gtgatgttga tcaagcgact ggcatcatcg ctgaaagcgg tgaaaccgga cagttgtgtg
     5221 gtgcgtgaag cgcaggactg gctgcgcgac agaaaacttg tggatatcac agatattctc
     5281 cggtgaacca ggcatgtata ttttacgcag ggacatgccg gtcatgatcg tttcgttcca
     5341 ggggtggtct ggagatcatg cggtgtgctc cctgctctcg ccggaataag gacgtcgccg
     5401 ccggctccgc ttttacccgg ccatgcgggg catggccttg tgggttttca gttatgtggc
     5461 cgccgtgtcg tgtgcgggct gtgccgtgcc tccatcgtag ccgggcttct gcgggcggga
     5521 tgcaaggtaa gatgaaccgc tgcggtcacc cggtccctcc ttcccgtctg ccgtgatttt
     5581 ccggtcgttc acccgctcag atttcgcggt ctcaccctcc aactcccgcc agccgtgtgc
     5641 gtcgggctgc ggcttccctt gcgcccttgc atccctgcag gcccggcaaa gatggaggca
     5701 cggcaccgcc cggtgtcgca gaacatgtga ctatggagga ttcgggaatg tctgttgttg
     5761 cacctgctgt atacgttgga acctggcaca aatacaactg tggaagcatc gctggccgct
     5821 ggtttgacct gacaacgttt gatgatgaac gcgacttttt cgccgcctgc cgtgcccttc
     5881 accaggatga aaccgacccg gaactgatgt ttcaggatta tgagggattc ccggggaata
     5941 tggcctctga atgccatatc aactgggcct gggttgaagg cttccgccag gcgcgggatg
     6001 aaggctgcga agaggcttat cgtctctggg tggatgatac cggtgagacg gattttgaca
     6061 gcttccgcga tgcctggtgg ggcgaggctg acagtgagga ggcttttgcg gttgagttcg
     6121 tcagtgatac cggcctgctg gctgacgtgc cggagacggt ggcgctctat tttgactatg
     6181 aggcgtatgc gcgggattta ttcctggact ccttcacctt tattgacggt catgtgttcc
     6241 gtcggtgatt tactccccgc tccggcgggg ggatggcctg ccaggccccc ggaaaaccct
     6301 ggcaggctta cgcctgcctg aaatacagga gggccgcgcc tgcggcgcta cccttcctgc
     6361 ctgtcatagt gtgaggcatt tttctgtttt gtcgtttctg ttgttctgac tcccgggggc
     6421 ggggaca
//
LOCUS       DAZFRE010000180         6248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFRE010000180 DAZFRE010000000
VERSION     DAZFRE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.182"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            53..601
                     /locus_tag="ACHKUI_004857"
     CDS             53..601
                     /locus_tag="ACHKUI_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6600875.1"
                     /translation="MNVNKKKLADIFGVDVRTITAWQSQGLPLVSGGGKGTESVFDTT
                     AAIQWYAQREADIKNEKLRKEIEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPDISPVHLDAVKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            573..2501
                     /locus_tag="ACHKUI_004858"
     CDS             573..2501
                     /locus_tag="ACHKUI_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6600876.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYC
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2485..2691
                     /locus_tag="ACHKUI_004859"
     CDS             2485..2691
                     /locus_tag="ACHKUI_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO6600877.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            2688..4280
                     /locus_tag="ACHKUI_004860"
     CDS             2688..4280
                     /locus_tag="ACHKUI_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6600878.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETATK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4270..5775
                     /locus_tag="ACHKUI_004861"
     CDS             4270..5775
                     /locus_tag="ACHKUI_004861"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6600879.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPE
                     RTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVQGGVSQASEDGDADVKMLMAMP"
     gene            5812..6159
                     /locus_tag="ACHKUI_004862"
     CDS             5812..6159
                     /locus_tag="ACHKUI_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6600880.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     GATGKLVVWDGQKAGSAVGILVLPLEGTEAVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFTGSALSHAALP"
BASE COUNT         1430 a         1521 c         1947 g         1350 t
ORIGIN      
        1 tttttgttag aaaacgccat tttcagatcc tttctggttc ccgggggagt gtatgaacgt
       61 caataagaaa aaactggccg atatttttgg cgttgatgtc aggaccatca ccgcctggca
      121 gagtcagggg ttaccactag tttctggtgg agggaaaggg actgaatcag tttttgatac
      181 aactgctgcc attcagtggt atgcgcagag ggaagctgat attaaaaacg aaaaactccg
      241 taaagagatc gaggatttga gggctgccag cgaatcagac cttcagcccg gcaccattga
      301 ttacgaacgt taccgactga cgaaggcaca ggccgatgca caggagctga aaaatgctcg
      361 tgaggaaggc cttgtcctcg agacggagtt atttacctac atctttcagc gagtggcaca
      421 gaatatatca gggatccttg tccgtgtccc tcagacactg cagcgtaaat accctgatat
      481 atcacccgta catcttgatg ctgtgaaaac tgaaatcgcg aaagcatccg atgtggcttc
      541 tgaagccggt gagaatgtgc gcaggtggat tgatgatttc agacgaactg agggcggcta
      601 attctgcagg agcgatagca accggcctcc ttgcgctaaa aattcctgtc cctctgacga
      661 cagttcagtg ggcagatcga cattattacc ttccgaaaga gtcatcttac accccggggc
      721 ggtgggaaac actgccgttt caggttgcca tcatgaacag catggggaat gaccggatcc
      781 gcactgttaa tctgattaaa tctgcccgtg ttggttatac aaagatgttg ctgggagtgg
      841 aggcttattt tattgagcat aaatcacgca acagccttct tttccagccc acggattctg
      901 ctgctgaaga ttttatgaaa tctcatgtgg aacccacgat cagggatgtg ccggttttac
      961 tcgatcttgc accgtggttt gggcgtaaac atcgtgataa taccctcacg ctgaaacgtt
     1021 tttcatcggg cgtgggcttc tggtgcctgg gcggggctgc cgctaaaaac taccgtgaaa
     1081 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgaaccggat gtcgaaaaag
     1141 agggttcgcc aaccctgctt ggggataaac gtattgaggg ctcggtatgg ccaaaatcca
     1201 ttcgcggctc gacgcctaaa ataaaaggct cctgccagat cgaaaaagcg gccaacgagt
     1261 cggcgcattt catgcgtttt tatgtgccct gcccacactg tggggaggag cagtatctga
     1321 aatttggcga tgaatccacg ccttttggcc ttaaatggga gaaagacagc cccgaaagcg
     1381 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgagctt gaccagagca
     1441 acgggcggtg gatctgtgaa aacacgggga tgtggacccg tgacggtctg acgtttttca
     1501 gcgccgcgga taatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
     1561 acagtccgtt caccacctgg gtacagatag tctatgactg gctggatgca ctgaaagatc
     1621 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
     1681 gcgaaaaact cgatcaccag gtactgatgg ataaggtcgt gcattacacg gcggcggtgc
     1741 cagcccgggt ggtttatctg acggcgggca ttgactcgca gcgaaaccgt tttgagatgt
     1801 atgtctgggg atgggcaccg ggagaggaag cttttctggt ggataaaatc atcattatgg
     1861 gccgtcccga tgaggaagag acgctgttac gtgtggatgc ggcgatcaac aaaaaatact
     1921 gccatgcaga cggaaccgaa atgaccattt cccgtgtctg ctgggacacc ggggggatcg
     1981 atggtgaaat tgtctatcag aggtcaaaaa aacacggtgt tttccgggtg ctgccggtaa
     2041 aaggtgcatc tgtttatggc aagccggtga tcaccatgcc aaaaacccgc aatcagcggg
     2101 gcgtgtatct gtgcgaagtg gggacggaca ccgcaaaaga aattctctat gcccgtatga
     2161 aagccgatcc cacgcctgcg gatgaagcca cgtcgtatgc catccgtttt cctgatgatc
     2221 cggagatttt ttcgcagaca gaggcgcagc aactggtggc ggaagagctt gtggagaagt
     2281 gggaaaaagg aaagatgcgt ctgctgtggg ataacaaaaa gcggcgtaac gaagcgctgg
     2341 actgcctggt gtatgcctat gcggcattac gtgtgtccgt gcaacgctgg cagcttgatc
     2401 tggctgtact ggcaaaatcc cgggaagaag agacgacccg gccaaccctt aaagaactgg
     2461 cagcgaagct gtccggagga gtgaatggtt acagtcgctg aactacaggc gctgcgtcag
     2521 gcgcgccttg atttattaac cggtaaacgg gtggtgtctg tccagaaaga tggtcgcaga
     2581 attgaatata cggcggcctc tctggatgag cttaaccgtg cgatcaatga tgctgagttg
     2641 gtactgggga caacccgccg tcgccgtcgt ccgctgggag tgaggttatg aaacgaacgc
     2701 ctgtcctgat tgatgtgaac ggcgttccgc ttcgggagag cctcagctac aacgggggcg
     2761 gcgcaggatt tggcgggcaa atggcggagt ggttgccacc ggcgcagagt gccgatgcgg
     2821 ccctgctgcc cgcgttgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2881 ggatagcggc caatgcggtg gccctgcata aggatcacat tgtcgggcat atgtttctta
     2941 tcagctaccg cccgaactgg cgctggctgg ggatgcggga gaccgcgaca aaaagttttg
     3001 tcgatgaggt ggaggcggcc tggtcagaat acgcagaagg gatgtttggt gagatcgacg
     3061 tggaagggaa acgcacgttt acggaattta tccgtgaagg tgtgggcgtt catgcgttta
     3121 acggcgaaat ctttgtgcag ccggtctggg atacggagag cacgcaactg tttcgtacgc
     3181 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatc gggaaccgtt
     3241 ttctgcgggc cggtgtggag gttgatcgat atggccgtgc cgttgcgtac catatctgtg
     3301 aggatgattt tcctcgctcc gggagtggac gatgggaacg gatcccgcgt gaacttccca
     3361 ccgggcgtcc ggccatgctg catattttcg agccggtgga ggacgggcag acccgtgggg
     3421 ccaaccagtt ttacagcgtc atggaacggc tgaagatgct cgattccctg caggcaacac
     3481 agcttcagtc ggccattgtc aaggcgatgt atgcagcgac aattgaaagt gaactggata
     3541 ccgaaaaggc ctttgaatat atcgccggtg cgccgcaggg gcagaaggat aatccgctta
     3601 ttaatattct ggagaagttc tccagctggt atgacacgaa taacgtgacg ctgggcggtg
     3661 tcaaaattcc gcaccttttc cctggtgatg atctgaaact acagacagcg caggattcag
     3721 acaatggatt ttctgcgctt gaacaggcgc tgctgcggta tatcgccgcc ggtcttggcg
     3781 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3841 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg tcccggctgg
     3901 ccacgcagat gttttcctgc tggctggaag aggcacttct gcgggggatt atccgtccgc
     3961 cacgggcacg ttttgatttt tatcaggcgc gttcagcctg gtcacgggct gagtggattg
     4021 gtgccggaag aatggccatt gacgggctca aggaggttca ggaatcggtg atgcgtattg
     4081 attccggact gagcacgtat gagaaagagc tggcgctgat gggcgaggat tatcaggaca
     4141 ttttccgcca gcaggtcagg gaatctgcag agcgggaaaa agccggactc tcacgtccgg
     4201 tgtggatagc gcaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     4261 caacaccacg tgagacgtaa tctttcacac attattgcag cagcattcaa tgaaccgctg
     4321 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgcga gatgggggca
     4381 gcaagtcttt cggtaccgca acagcaggta cagcttgatg ctcccgggat gctggctgaa
     4441 acggacgagt acatggccgg aggtaaacga ccggcccgtg tttaccgggt ggtgaacggt
     4501 attgcggtac tgccggtgac cggcacgctg gtgcaccggc tgggggggat gcggccattt
     4561 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcagatagc
     4621 caggtgcggg gcatactgct ggacattgac agtccgggcg ggcaggccgc cggcgcgttt
     4681 gactgcgctg acatgattta ccgcctccgg cagcagaagc cggtctgggc actgtgcaat
     4741 gacacggcct gttctgcagc catgctgctg gcgtcggcct gctcccgacg gctggttacc
     4801 cagacatccc gtatcggctc cattggcgtg atgatgagcc atgtcagcta tgccggtcat
     4861 ctggcgcagg ccggtgtgga tatcacgctg atttatgccg gggcgcacaa ggtggatggc
     4921 aatcagtttg aagcgttgcc ggcagaggtt cgccaggaca tgcagcagcg gattgatgcg
     4981 gcgcgccgga tgtttgccga aaaagtggcg atgtataccg gtctgtctgt tgatgccgtc
     5041 acgggaacag aggctgccgt ttttgaaggt cagtccggca ttgaggccgg gctggcggat
     5101 gaattaatca atgcgtcgga tgccatcagt gtgatggcca cggcgctgaa cagtaatgtc
     5161 agaggaggca ctatgccgca attaactgca acggaagccg ccgtgcagga gaaccagcga
     5221 gtgatgggga tcctgacatg ccaggaagcg aaaggacgtg aacagcttgc cacgatgctg
     5281 gcagggcaac agggcatgag cgttgaacag gcccgggcga ttctggccgc ggcggcaccg
     5341 cagcagccgg tggcatccgc acagagtgaa gccgatcgca ttatggcgtg tgaagaagcg
     5401 aacggtcgtg aacaactggc agcaacgctg gcggcgatgc cggagaggac ggtggaaaaa
     5461 gcccgcccga tcctggctgc ttcaccgcag gcgaatgccg ggccctcact tcgtgatcag
     5521 atcatggcac tggatgaggc aaaaggggct gaggcgcagg ctgaacagct ggctgcctgc
     5581 ccgggaatga ctgtggagag cgcccgggct gtgctggctg cgggatcagg taaggcagaa
     5641 ccggtctctg catccacaac cgccctgttt gaacatttca tggcgaacca ttcaccggct
     5701 gcggtccagg ggggcgtgtc acaggcgtca gaagacggtg atgcggacgt gaaaatgctc
     5761 atggccatgc catgaagtca gtgctgaaca tcaatacgag gttttaacaa tatggtgacg
     5821 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggctcac
     5881 accgccacag gcagcagcgg gatttcctcg gcaacaccgg cactgacacc cctgatgctg
     5941 gatggggcca ccgggaaact ggtggtctgg gacggacaga aagccggtag tgcggttggc
     6001 atactggtac tgccgcttga aggcacagag gcggtgctga cgtattacaa gtcggggacc
     6061 tttgcgacgg aggcaatccg ctggcctgaa agtgtggatg aacacaaaaa ggccaacgcc
     6121 tttaccggca gtgccctgag tcacgcggcg ctgccgtaac acgttatcag gccaccgcgt
     6181 tggcctgact gatttcttaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     6241 ttactcgg
//
LOCUS       DAZFRE010000181         6172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFRE010000181 DAZFRE010000000
VERSION     DAZFRE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.183"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(82..375)
                     /locus_tag="ACHKUI_004863"
     CDS             complement(82..375)
                     /locus_tag="ACHKUI_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600881.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            complement(394..603)
                     /locus_tag="ACHKUI_004864"
     CDS             complement(394..603)
                     /locus_tag="ACHKUI_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600882.1"
                     /translation="MTLYEILKAEFKTNAAIGRRFPKRGKPRGSQGVGKWKTRGVPED
                     VAILCHLDPKIPYQHPSLMNASHES"
     gene            714..1370
                     /locus_tag="ACHKUI_004865"
     CDS             714..1370
                     /locus_tag="ACHKUI_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6600883.1"
                     /translation="MRTLGERLSSARQRAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLLKVATALNCSPQWLQNGEEQSVNWENNVKSCPQKDAFHSYPVINWVQAGLFST
                     AGDDYSMYDHDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKAVHS
                     GCYVIARKKSTNEATFKKYVTEMGRSFLKPLNPQYPIIEIDDDCEIVGVIVDARWDIF
                     "
     gene            1455..1865
                     /locus_tag="ACHKUI_004866"
     CDS             1455..1865
                     /locus_tag="ACHKUI_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6600884.1"
                     /translation="MFQKTTLSCIEDLKNLGSLIALIAKAAPDTTLANDIESCAGMAW
                     DIINKVSRTLSLLIHKQNISDCPHEIRTQRESCGLSTAELATLLGVDEETIIQWENGK
                     AEPLASQVIPLANILDCDPMWLLTSFHSDTTKES"
     gene            1867..2058
                     /locus_tag="ACHKUI_004867"
     CDS             1867..2058
                     /locus_tag="ACHKUI_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600885.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            2055..2255
                     /locus_tag="ACHKUI_004868"
     CDS             2055..2255
                     /locus_tag="ACHKUI_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600886.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            2430..2792
                     /locus_tag="ACHKUI_004869"
     CDS             2430..2792
                     /locus_tag="ACHKUI_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600887.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            3296..3760
                     /locus_tag="ACHKUI_004870"
     CDS             3296..3760
                     /locus_tag="ACHKUI_004870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600888.1"
                     /translation="MEGYIVFPPFFSDAEYSAINNAGIDRAFHKYELDCGPVYRVCRH
                     GQVKKCFTRKTAIIHLAHFMTTKVFESSGFEKRLPDQRLMHPEYGEVFHPGEITPEYY
                     RAHGRCFYRLLRILARRKQFDAWMEKYNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            3747..4331
                     /locus_tag="ACHKUI_004871"
     CDS             3747..4331
                     /locus_tag="ACHKUI_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600889.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            4355..5221
                     /locus_tag="ACHKUI_004872"
     CDS             4355..5221
                     /locus_tag="ACHKUI_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO6600890.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            5214..5576
                     /locus_tag="ACHKUI_004873"
     CDS             5214..5576
                     /locus_tag="ACHKUI_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO6600891.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEELPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            5593..5817
                     /locus_tag="ACHKUI_004874"
     CDS             5593..5817
                     /locus_tag="ACHKUI_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600892.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            5814..>6172
                     /locus_tag="ACHKUI_004875"
     CDS             5814..>6172
                     /locus_tag="ACHKUI_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600893.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRV"
BASE COUNT         1911 a         1382 c         1417 g         1462 t
ORIGIN      
        1 tatgcactag cttggtagta cacaggtgca tcctgagaag tggggtctca gcccgtcggt
       61 cacctttaag accaacacca ttcaggtacg ctgttgcgtt cctgccaggg gcggctgagt
      121 cgtatacccc tgtaatgcac gcccgtgtgt ttcaacgaac ctgcgcagcc gtacattggc
      181 ttcatgcctc gctctgttct ccttacggaa tgaaacaggc tcggaatcaa aagtaacttc
      241 gtaaacctcc gcgtatttca gggcgacctt ctgtctcagt gaggaaggca ggctctgtaa
      301 ctgccgctta atccactctg aatctgcctg gctgtacctg gcaggcatct ccatctgaac
      361 gtaatcaggg gacatacagc ctccggcaga acatcacgat tcatgactgg cattcatgag
      421 cgacggatgc tgataaggaa ttttgggatc cagatgacaa aggatcgcca catcttccgg
      481 cacacctcgg gttttccact ttccaacgcc ctgactacct ctaggttttc ctctcttagg
      541 gaaacggcga ccaattgcag cattggtttt aaactcagct tttaaaattt cgtaaagagt
      601 catctctcac tcactgattg atactttagc atccataaat atacaacaaa tgaatctaaa
      661 gtatcaagaa tatgtgctac tttagtatcg accgaaaaaa caggagagat aaaatgagaa
      721 ctttgggaga gcgccttagc agcgccaggc aacgggcagg attgacacaa gatgcattag
      781 caaaaaaagc cggcataacc cgcgtggcta taagcaaagc agagcaagga cttactaaaa
      841 gcttcaacgg agatacttta ttaaaggtag ccacagccct aaactgctct ccacaatggc
      901 tacaaaatgg agaagaacaa tctgtaaact gggaaaacaa tgttaagagt tgtcctcaaa
      961 aagacgcatt tcattcctat cccgttatta actgggtcca ggccggacta ttctctactg
     1021 ctggagatga ttacagcatg tatgaccatg ataactggag acattctgtc aaatatgcag
     1081 gtgaaagagg tttttggctt gaagtacatg gtgattcaat gacctcgcct gttggaatca
     1141 cctttcctga gggtatgtct attctcgtaa acccagacaa agctgtacat tcaggctgtt
     1201 acgttatcgc aagaaaaaaa tcaaccaacg aagcaacatt caaaaaatat gtgactgaaa
     1261 tgggaagatc tttcctgaaa ccactcaatc ctcagtatcc catcattgaa atagatgatg
     1321 attgtgaaat cgtcggcgtg atcgtggatg cccgatggga tattttctaa gaaagatact
     1381 ttagtatcaa aaaacgcttg ctcatgaatg atactttagt taccatgcta aaaagtaact
     1441 gaggtatcat taatatgttc caaaaaacta cgctatcatg cattgaagat ttaaaaaatc
     1501 ttggctccct cattgctctt atcgcaaaag cagcgccaga tacaactctt gccaatgata
     1561 tcgagtcatg cgccggaatg gcgtgggata taataaacaa agtatctcgc acattatctt
     1621 tgttaattca taaacaaaac atttctgatt gcccccatga gatccgcacc caacgcgaaa
     1681 gctgcggtct atccaccgct gaactcgcca cgctgcttgg agttgatgaa gaaaccatca
     1741 tccagtggga gaacggcaag gctgaacctc tggcaagcca ggtgattcct ctggcaaata
     1801 tacttgactg cgatccgatg tggttactta caagtttcca ttctgacacg acaaaggaat
     1861 cgtaagatga aaatgttcaa aggcctcacc aacgaaccgg aaacagcttt tcatcacatt
     1921 gcagtactgc ttgaagcggg gttaatcatt tcggcttccg gtgatgaaga atgtgatgaa
     1981 ctttcggatg acatattttt actggcacaa caatacgcca gaagcgcatg cgatgcattt
     2041 aaggaacaaa gaacatgaaa actcccctga ttatgcttga ggaagtggca gcagaaataa
     2101 aagaaaacac gtcaatgctt gagtttatat ttaaaaactc tggtgataac ggagagacag
     2161 atgatttctt actttgtatg attcgttcca tgaataaaac ctgtgaaaag gcttacgagt
     2221 atgttgatgc tctgagaacg aataaaggaa attaaacaga caatcataca taaaaacatg
     2281 acggtctgcg gtcgggagtt ctcactattt taaaatgggg atgacacatg aaagacaaac
     2341 atgcctataa aaccgcttta ttaatggctg atgccggata ctggtcagtt gcagtattgt
     2401 ttctcaaaaa ggcatacggg aaataacaaa tgaaagatga atacatttct gcatatcgtc
     2461 gccgcataac ggttgcagca ctgcggcgga tgaaacgaaa aacaggaagt aacctcctca
     2521 ctgtaaagcg tcatgatgga gagttaacaa ctattgaaat aacagaacag ttcataagcc
     2581 agttgctatt acgatttgaa ggcgttaccc gtggtgagtt tgggcggaaa gatggtgaaa
     2641 cagcaatacg cactgcatac caggatgctg tcggtatcaa ccaacatggt gagtatctga
     2701 ctgaatcagg aaagttaatc attgatgaac ttttgaatga atgcatcgat tacattaaag
     2761 aggggcatat taccagatgc gttaatcatt aacagcatag ctcctctccc ggaataacgg
     2821 agataatgaa aaatgaataa ttgcgttgaa gtactcgata atgtccactg cgagataaca
     2881 gaaagcctgt ctttgttaca ggtgattatt gacagcggtg atattggttc accagatgcg
     2941 ctaaacatga tgaattgtct tttccgctca ttatcctgta cagcagacaa agcagaaaag
     3001 catctgtgcc ggactgaaaa cgaataacat tatttaagaa cagtaattaa tttacagctt
     3061 tattgtcggg gatttttaca acctgaaaag gccaggctgt atcacagata tatcgcaaca
     3121 aacgaattgc tgagccacat cttctggctg cgctcagagt gaataaaagt aattaacacc
     3181 ggatgaacac ggagtaacca tgaaagaaga acctgaaatc attgaaatta attctgaaga
     3241 attcacagaa aacgaatgac tgacagaaac cgatccgcgc gttctggcgc tgacaatgga
     3301 aggatacatc gtatttccac ctttcttcag tgatgcggaa tacagtgcaa taaataatgc
     3361 cggaatcgac cgtgcttttc ataaatacga actcgattgc ggccctgttt acagagtctg
     3421 ccgccacgga caagttaaga aatgcttcac gcgaaaaacg gccatcatac atctcgctca
     3481 ttttatgaca acgaaggtat tcgaaagttc aggttttgaa aaacgtctgc ctgaccagcg
     3541 cctgatgcat ccggaatatg gagaagtctt tcatccggga gaaattacgc ccgaatacta
     3601 cagagctcac gggcgttgtt tttatcgcct gttgcgcatc cttgcacgca gaaaacagtt
     3661 tgatgcctgg atggaaaaat acaacgcctg gattgatcag tattgccggc ttgtcgcgca
     3721 aagaccattt aagaagagag caaaccatga ctgcaactga acagttattt tttcttaccg
     3781 ctgaaggtat ccacacacct tacggactgc ttgatatcaa caaagcactg gaaatgctcg
     3841 atgaaggctg ttttgacgag ccgatctggc atggtctgga aatagtgtgt tgcatcagtg
     3901 aagcagaaga aaaaggcatg acaggtgagc tcgcgccgga aaagacaatc atgtactggc
     3961 gctggcttta taccgccatg ttcttctctg aacagctcac cagaaacggc tggaccgaag
     4021 tgcttaacga gaatggcggt acagacaagg gcaccatgta cagaggccac aacggtggca
     4081 tgacaatcta cctgaaacca ttacgtctcg ctatgcagaa ctatatggaa tgggcactca
     4141 tagaaactta cggagcggaa tcagggctgc tcaaaagcat tcttatgtac aaagcattca
     4201 ccggcgctct gggcgacggc gaaaaaaccg gcatcacaat ggaaggttac agagcgatgc
     4261 acatgattct ggatgggctt atcagtgaag ccactgacaa ctcagaaccg gaaaccagag
     4321 tgttccacta atcagctttt cgtgaggtgt aatgatgaaa aaatcaactg aacttgtgcc
     4381 ggtggcagcc cgtgatttgc aaattatcga atatcgcggt caacgcgttg tgaccactga
     4441 acaactggcg gcaggttatg gagcaacacc aatccgtatt cgccagaatc attcccgaaa
     4501 cgaagaacga ttcgttgttg gaaagcatta ttttttgctg gaaggcgatg aactacgtga
     4561 acttaagcac agagtatctc aaagctactc tgtgaaaatt gccagcaatg ttcgatccct
     4621 catcctctgg accgaacgcg gcgcagccaa ccacgcaaaa ttgctggaaa cggatcaggc
     4681 gtggaactat cacgaagacc ttgtggagtt ctacttcact cagcgggaaa acatcgatgt
     4741 tcagcgcgta ctcactcgca aggaactcgc gttgatggtt attgaggccg aagaacgggc
     4801 tgaagctatg gccctggaaa ataaaacgct ctctgcaacc gtagacagtc tggaaaaaca
     4861 tttctctaaa ggcatgactg ttccccagtt ctgccgtgcg ctgaacggcg taaataccag
     4921 ccagataatg tggtggctgt ctggccgaaa atgggtattc aacgaacaac gcgatccgga
     4981 gaaaacaccc cgctggcgca cggcctctta tgcccgtgac agatacctga ccgaagaagc
     5041 agtacagatc actccacacg ggaaagcaac attcacgaaa ttcacccccg tattgctgga
     5101 aaaaggatgc caccgtttat accagtttta catgaacggt gaactcccta tgaagaaaaa
     5161 ctggaacggt gaatttaaac acgacaaagc tatttacaca ccggaggcag aacatgaata
     5221 gcactactgc ggcaatcatg aactacgacc cgaacatgac attatgcggc cgcatggcaa
     5281 gacagacggt tcgtctgaca ctcgggcaat gggagtaccg cgaaacattt gaagtggacg
     5341 ttgtcggcaa tctgacgggg ctggatgtaa taacctgcgc aattgaaaac ctttatgaag
     5401 agctgccgta tgaagaagca caggatgaat ataccggtga aactaacatt atggccacaa
     5461 tacagatcgg tgatcttgca tgtacggatg aggacatgct cggcgaagca tggctggcag
     5521 gtatgcttat tgcagcagaa attatcagca ttgaaccagc aggaacactt tcataacgga
     5581 gatcgccatc agatgattat ttcattcacc gattacggcg aacaggcaaa actgattatc
     5641 accagttccc gcctgacatt gcgaaaacat aatcgtgtcg ttgatgcggt acttctgtgc
     5701 acgccggaca tcacagaaag ccggagcggc tttttcctgg ttaagtcgtc attatcagga
     5761 aaaacacgcg acatcctgcg cgcctacaaa acagcaacaa gggagtctga acgatgaaag
     5821 aagctgatct gaaagcactg cgtgacgccg cacaaaatac cgtatatgga gacaagggat
     5881 gtgatctgga agcatttcac tcccttgcca ctccgccagt tatccttgcg ctaatcagtc
     5941 acatagaaaa tctggagaaa aaactggatg aaatcgtaac gtcagagatt agcccgaagg
     6001 cctggctgaa taaccttacg ggggaagttt gcagtattga tgccatgcct gacgcccgta
     6061 atgacagaga aacatacacc ccgctttatc ctgcctggca gcgcattgcc gccgcagatg
     6121 ataacggcag agtggcatgg atgtatacgc acaaaaaagc aggttacagg gt
//
LOCUS       DAZFRE010000182         6149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFRE010000182 DAZFRE010000000
VERSION     DAZFRE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.184"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1602
                     /locus_tag="ACHKUI_004876"
     CDS             <1..1602
                     /locus_tag="ACHKUI_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6600894.1"
                     /translation="QMQGKAQSIQDVLAGLEERRVALIRQQAAEQNKAYQSLLIMNGQ
                     HTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQTALNNSERELALSRLKGEAR
                     ERARLGYAADDLGFVGEAYQTARLNYINNSLDAWRNNQANKPKAHKKTEAEKTEDIYK
                     RLIKQQKEQIALAGQNTELAKMKYQVSQGELSTLSEAQKKTLLQNAALIDQKKIREQL
                     AAYESSLADSNASVRASNEAQLLGYGEGSRMRERLQEMWSIRQEFEQKNNELLRQYQA
                     GEIEEALWKQEKELNKKYLEERLSDQQDYYVKADALRSNWNAGLKEGLTNWADSATDY
                     ASQAADAVVSTMDGLVSNISDALAGNVVDWRNWGSSILQEVSKILMNAAIVNGLKSLS
                     KSMSGAGGWLGTVGDWLSGAVANAKGGVYTSANLSAYSNTIVDTPTYFAFAKGAGLMG
                     EAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVYSPVYHISIQNKGSNGEIDTQSAKG
                     LVDLIDSRVVSIMQSSRRDGGLCSA"
     gene            1595..1936
                     /locus_tag="ACHKUI_004877"
     CDS             1595..1936
                     /locus_tag="ACHKUI_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6600895.1"
                     /translation="MPESEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGVRAFLWRPPKHNRTVRVVCREWSISDNAMYT
                     DFNCTFEEVTH"
     gene            1936..2634
                     /locus_tag="ACHKUI_004878"
     CDS             1936..2634
                     /locus_tag="ACHKUI_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6600896.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDVVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRTMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            2639..3382
                     /locus_tag="ACHKUI_004879"
     CDS             2639..3382
                     /locus_tag="ACHKUI_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6600897.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGATDCYTLFRDAYHLAGIEMPDFHREDDWWCNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            3280..3927
                     /locus_tag="ACHKUI_004880"
     CDS             3280..3927
                     /locus_tag="ACHKUI_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6600898.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     STQLPAFRQKLNDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTATSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            3988..>6149
                     /locus_tag="ACHKUI_004881"
     CDS             3988..>6149
                     /locus_tag="ACHKUI_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6600899.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REIMLSSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRIPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGAYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTAETTYRFRQ
                     LALGRYTLTVRAVNARGQQGDPASVSFRINAPAKPATI"
BASE COUNT         1448 a         1491 c         1948 g         1262 t
ORIGIN      
        1 caaatgcagg gcaaagcgca atccattcag gatgtgcttg ccgggctgga ggagcgacgg
       61 gtggcgttga tccgtcaaca ggcagcggaa caaaacaaag cgtatcagtc cctgttgatc
      121 atgaatgggc agcataccga gtttaatcgc cttctcgggc tcggtaatga attacttcag
      181 cagcgacagg ggctggtgaa tgtaccgtta cggctaccac aggcaaccct ggatgataaa
      241 cagcagaccg cactgaataa cagcgagcgc gaactggctc tgtcccgcct gaagggggaa
      301 gctcgtgagc gtgcccgcct gggttatgct gcggatgatc tcggatttgt gggagaggcg
      361 tatcagacag caagactgaa ttatataaat aactcactgg atgcatggcg aaataaccag
      421 gcaaataaac ccaaagcgca taaaaagacc gaagcggaaa aaacagagga tatttataaa
      481 cggctgatta aacagcaaaa agaacagata gcactggcag ggcagaatac tgaactggct
      541 aagatgaaat atcaggtcag tcagggcgaa ttatcaaccc tgtcagaagc gcagaaaaaa
      601 acgcttttgc agaatgcagc acttatcgac cagaaaaaga ttcgtgagca gcttgctgcg
      661 tatgagagca gtctggcgga cagtaatgcc agtgtcagag catcaaatga ggctcagtta
      721 ctgggatatg gtgaaggctc acggatgcgt gaacgacttc aggaaatgtg gagcatccgg
      781 caggagtttg agcagaaaaa taacgagcta ctgagacagt atcaggccgg agaaattgaa
      841 gaagccctgt ggaaacagga gaaagagctg aataaaaaat atctggaaga gcgtctcagc
      901 gatcagcagg attattatgt aaaggctgat gctttacgca gtaactggaa tgccgggctc
      961 aaagaggggc tgacgaactg ggcagacagt gccaccgatt atgcttcgca ggcggcagat
     1021 gctgtcgttt ccacgatgga cgggctggta tcaaatattt ccgatgcact ggccggaaat
     1081 gttgtggact ggagaaactg ggggagttca attctccagg aagtttcaaa aattctgatg
     1141 aacgctgcca tcgttaacgg gctgaagtca ctttccaaaa gcatgtccgg tgccggagga
     1201 tggcttggta cagtcggcga ctggctttcc ggtgcagtgg caaacgcaaa aggtggtgtt
     1261 tatacatcgg caaatctgag tgcttacagt aacaccattg tggatacccc gacgtatttt
     1321 gcttttgcga aaggtgccgg gctgatgggc gaggccgggc ctgaagctat catgccactg
     1381 actcgggcag cggacggctc tcttggagtc agagccattg gcaatgtgaa tggtggcggt
     1441 ggatttgttt attctcccgt atatcacatc agtattcaga ataaagggag caatggcgag
     1501 atagatacgc agtcagccaa gggactggtg gatctgatcg acagcagggt tgtgtcaatt
     1561 atgcagtcat cacgtcggga cggaggatta tgcagtgcct gagtctgaag tttttaactg
     1621 gatcccccgc gaggggatgg agacgacacg aaagccatct gttattacgg taaagttcgg
     1681 tgacggatat gaacagcgac gggctggtgg tctgaatgca gatctgaaaa cgtttaaacc
     1741 ggtatttcgt gtcacggatg aatattcccg tgccgcgctg gacagttttt tatcccgtca
     1801 tgccggggtt cgtgcttttt tgtggcgtcc gccaaaacac aacaggactg tccgggttgt
     1861 ctgcagggag tggagcattt cggataatgc catgtatacc gattttaact gtacctttga
     1921 agaggtcact cactgatgca ggatatacag caggaaacac tcaatgagtg cactaaaacg
     1981 gagcaatccg cgctggtcgt gctctgggaa attgatctga cagaggtcgg cggagatcgt
     2041 tatttcttct gtaatgagca gaacgaaaaa ggtgaaccag tcacctggca ggggcggcag
     2101 tatcaggcct atcccattca gggaagtgga tttgagatga acggcaaagg agccagtgca
     2161 aggccaacgc tgaaagtctc taatctgcac ggcatggtca ccgggatggc ggaagacctg
     2221 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgtg
     2281 gtgaacttcg tcaacggaaa cagcgaagcc gatccagagc aggaggtgat cagccgctgg
     2341 cgcatcgagc agtgcagcga actgagcgcg gtgagtgcct cttttgtact gtccacgccg
     2401 acggaaacgg atggtgctgt ttttccggga cgtaccatgc tggccaacac ctgcacctgg
     2461 acctatcgcg gtgatgagtg cggttatcac ggtccggcgg ttgcggatga atatgaccag
     2521 ccgacgtccg atatcacgaa ggataaatgc agcaaatgcc tgagtggttg taagttccgc
     2581 aataacgtcg gcaactttgg cggcttcctt tccattaaca aactttcgca gtaatcccat
     2641 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     2701 cggcttcgtg gtgagaacgc cggaagggga aagatatttt ccctgcgtga atatctccgg
     2761 tgagccggag gcgtatttcc ggatgtcgcc ggaggactgg ctgcgggcag agatgcaggg
     2821 tgagattgtg gcgctggtcc acagccaccc cggtggcctg ccctggctga gtgaggctga
     2881 caggcggctg caggtgcaga gcgatttgcc ttggtggctg gtctgccggg gtgagattca
     2941 taaattccgc tgtgtgccgc atcttaccgg gcggcgcttt gagcacgggg cgacggactg
     3001 ttacacgctg ttccgggatg cttatcatct ggcggggatt gagatgccgg actttcatcg
     3061 tgaggatgac tggtggtgta acggccagaa tctctatctg gataatctgg aggccacagg
     3121 gctgtatcag gtgccgttgt cagcggcgca gccgggcgat gtgctgctgt gctgttttgg
     3181 ttcatcggtg ccgaatcatg ccgccattta ctgcggcgac ggtgagctgt tgcaccatat
     3241 tcctgaacaa ctgagcaaac gagagaggta taccgacaaa tggcagcgac gcacacactc
     3301 cctctggcgt caccgggcat ggcacgcatc tgcctttacg gggatttaca acgatttggc
     3361 cgccgcatcg accttcgtgt aaaaacgggg gctgaagcca tccgggcgtt gtccacacag
     3421 ctcccggcgt ttcgtcagaa actgaatgac ggctggtatc aggtgcgcat tgccgggcgt
     3481 gatgcaggtg aaaccgaatt atctgcccgt cttaatgagc cgctggcaaa tggtgccgtg
     3541 atccacatcg tgccgcgtct ggcgggagct aaaagtggcg gtgtgtttca ggtggtgctg
     3601 ggggcggcgc tgattgcggt ggcatggtgg aaccctgtgg gctggctggg tgccgcggct
     3661 gtatcgggca tgtatgcggc aggggccagt atgatcctgg gtggtgtggc ccagatgctg
     3721 gcaccgaaag cccggacgcc cacagcgacc agcacggata acggtaagca gaacacctat
     3781 ttctcatcac tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa
     3841 atgcgcgtgg ggtctcgtgt ggtttctcag gagatcagca cggcagatga aggggacggt
     3901 ggtcaggttg tggtgattgg ccgctgatgc aaaatatttc atgtgaaacc gcctccgggc
     3961 ggttttgtcg tttatggagc atgacgaatg ggcaaaggaa gcagtaaggg gcataccccg
     4021 cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga tgccatcagc
     4081 gaagggccga ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
     4141 gtgctggaca gtgaggggaa taccaacatc tccggcgtca cggtggtgtt ccgggcaggt
     4201 gagcaggagc agacaccgcc ggaaggtttt gaatcctccg gctccgagac ggtgctcggt
     4261 acagaagtga aatatgacac gccgatcacc cgcaccatca cgtcggcaaa catcgaccgt
     4321 ctgcgcttta ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg tgaccggaat
     4381 ccgtcggaag tccgcctgct ggttcagata cagcgtaatg gtggctgggt gacggaaaaa
     4441 gacatcacca ttaaaggcaa aaccacctcg cagtatctgg cctcggtggt ggtggataac
     4501 ctgccgccgc gcccgtttaa tatccggatg cgcaggatga cgccggacag caccacagac
     4561 cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc
     4621 tacccgaaca cggcactggt cggcgtacag gtggactcgg aacagttcgg cagccagcag
     4681 gtgagccgta attatcatct gcgcgggcgt attctgcagg tgccgtcgaa ttataacccg
     4741 cagacgcggc aatacagcgg tatctgggac ggaacgttta aaccggcata cagcaacaac
     4801 atggcctggt gtctgtggga tatgctgacc catccgcgct acggcatggg gaaacgtctt
     4861 ggtgcagcgg atgtggataa atgggcgctg tatgtcatcg gccagtactg cgaccagtca
     4921 gtgccggacg gctttggcgg cacggagccg cgcatcacct gtaatgcgta cctgaccaca
     4981 cagcgcaagg cgtgggatgt gctcagtgat ttttgctcgg cgatgcgctg tatgccggta
     5041 tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaggt gtggacctat
     5101 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc
     5161 ctgaaggacc gccataatgc cgttgaggtg aactggattg acccggataa cggctgggag
     5221 acggcaacag agcttgtgga ggacacgcag gccattgccc gttacggtcg taacgtcacg
     5281 aagatggatg cctttggctg taccagccgg gggcaggcac accgcgccgg gctgtggctg
     5341 attaaaacag aactgctgga aacgcagacc gtggacttca gcgtcggcgc agaagggctt
     5401 cgccatgttc cgggcgatgt cattgaaatc tgtgatgatg actatgccgg tatcagcacc
     5461 ggcgggcgcg tgctggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc
     5521 atgctgtcat cctccggcac cacgctgata agcctggttg acggaagtgg gaatccggtc
     5581 agcgtggagg tccagtccgt caccgacggc gtgaaggtga aagtgagccg tattcctgac
     5641 ggcgttgccg aatacagcgt gtgggggctg aagctgccga cgctgcgcca gcgcctgttc
     5701 cgctgtgtga gtatccggga gaacgatgac ggtgcgtatg ccatcaccgc cgtgcagcat
     5761 gtaccggaaa aagaggccat cgtggataac ggagcgcact ttgacggcga ccagagcggc
     5821 acggtgaatg gtgtcacgcc gccagcggtg cagcacctga ccgccgaagt caccgcagac
     5881 agcggggaat atcaggtgct ggcgcgctgg gacacgccga aggtggtgaa gggcgtgagc
     5941 ttcctgctcc gtctgaccgt aacagcggat gacggcagtg agcggctggt cagcacggcc
     6001 cggacggcgg aaaccactta ccgcttcagg cagctggcgc tggggcgtta cacgctgacg
     6061 gtccgggcgg taaatgcccg gggacagcag ggcgatccgg cgtcggtatc gttccggatt
     6121 aacgcacctg caaaacccgc caccattga
//
LOCUS       DAZFRE010000183         6071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFRE010000183 DAZFRE010000000
VERSION     DAZFRE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.185"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..243)
                     /locus_tag="ACHKUI_004882"
     CDS             complement(<1..243)
                     /locus_tag="ACHKUI_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600900.1"
                     /translation="MGNVRFLQGVKKYFCTKALDVILLIYLLLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNS"
     gene            384..2816
                     /gene="hsdR"
                     /locus_tag="ACHKUI_004883"
     CDS             384..2816
                     /gene="hsdR"
                     /locus_tag="ACHKUI_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO6600901.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWRKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDEEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLNDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2883..4352
                     /locus_tag="ACHKUI_004884"
     CDS             2883..4352
                     /locus_tag="ACHKUI_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6600902.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4352..>6071
                     /locus_tag="ACHKUI_004885"
     CDS             4352..>6071
                     /locus_tag="ACHKUI_004885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6600903.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRLGDFTNIIRGITFPGNEKSQFQAPGKIACLRTANVQEQIDWDDLIYISDSF
                     VKRDDQYLQEHDIVMSMANSRELVGKVALASLPDNSKFTFGGFLSVLRPLVVNEIYLM
                     ALLRCETYKSQLIESASQTTNIANISLAKLNPLPVCIPPAKEQIHIVKKMNELMSLCD
                     QLEQQSLTSLDAHQQLVETLLGTLTHSQNAEELAENWARISEHFDTPFTTEASVDALK
                     QTILQLAVMGKLVLQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPF
                     ELPEGWEWCRLNDISSKITDGDHKTPPRIAEGYKLLSAKNIRDGYLDYNNCDYISAID
                     YEKSRERCLPEKGDLLIVSVGGTIGRSSLIKDCSDFALVRSVAIIKPLLIEPEYLKLA
                     MDSKLLQSMIHSHKRGGAQPCLYLGEISKFLFPTPPLAEQRNIVNKVSILMEKCRFLF
                     LGLQSAQ"
BASE COUNT         1837 a         1405 c         1406 g         1423 t
ORIGIN      
        1 actattcaac ctgtttttat tactgaactt aacaaaatac cccatatatt ctgtaatgcg
       61 attttcttcc actaatgcat aaagaagaat accaacaaag cacagagata aaataatatc
      121 tttccacaga tatttataag ggagaagtat aaaaccaagg agtagataaa ttagcagaat
      181 gacatctaat gccttagtac aaaaatattt tttcactcct tgcaagaacc gaacattccc
      241 catatgaatc cttatccagc tattgatgaa actcaagaat acctttaaaa cagtgaatta
      301 acgctgataa tactctttga acagatgaat tcatctattt ttctgtgtag aatttgcgca
      361 tcgcatttag atgagaacac cccatggcgg aactgaacct aagtaacctg acggaagcag
      421 acatcattac caaatgcgtt atgccagcca ttctcaatgc aggctgggac aacacaacgc
      481 aaatcagaca ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg
      541 cacgcagaac ggtaaaatct gctgacatcg tgctgtatca caaaccaggc attcccttag
      601 ccgtgattga agcaaaagcc aacaaacatg aaattggcaa agggatgcaa cagggcattg
      661 aatatgcgcg cctgctggac gttccttttg tttttgccac caacggggat ggctttatct
      721 tccgcgatgc caccgcagcc gaaggtgaat gccttgaaaa gcaaataacg ctggatgact
      781 tcccctcccc cgctgaactc tggcgaaaat tctgcctctg gaaaggatac acacaagctc
      841 agcttccggt gattactcag gactattacg acgatggcag cggtaaatcg ccacgttatt
      901 accagcttca ggcaatcaac aaaaccattg aagccgtctc caacgggcaa aaccgcgttc
      961 tgctggtcat ggcgaccgga acagggaaaa cctataccgc attccagatc atctggcgcc
     1021 tgtggaaatc aaaaaataaa aaacgcattt tgttccttgc cgatcgcaac attctggtcg
     1081 accaaaccaa aaataatgat ttccagccat ttggtacggc aatgaccaaa gtcagcggac
     1141 gcaccattga tcccgcttat gaaattcacc tcgcgctcta tcaggctata actggcccgg
     1201 aggaagacca aaaagcgttt aaacaagtcg caccagattt cttcgatctg atcgtgatcg
     1261 acgaatgcca tcgcggcagc gcatccgaag acagcgcctg gcgagagatc cttgattatt
     1321 tcagttccgc cacccaaatt ggcttaaccg ccacgccaaa agagacgcat gaagtctcca
     1381 gcacggatta cttcggcgat ccggtttacg tctactcact aaaagaaggg atcgaagacg
     1441 gcttcctcgc cccttataaa gttgtccgtg ttgatattga tgttgatctg caaggctggc
     1501 gcccaaccaa agggcaaact gacttaaacg gcgaagtgat cgacgatcgt atctataacc
     1561 agaaagattt cgatcgcacg atggtaatcg acgaacgcac agaactggtt gccagaacca
     1621 ttaccgacta cctcaagcgc accaacccga tggataaaac catcgtcttc tgtaacgaca
     1681 tcgatcatgc agaacgtatg cgccgcgccc tggtgaatct caacccggag caggtgaaaa
     1741 agaacgacaa atacgtcatg aaaatcaccg gcgatgatga aattggcaaa gctcagttgg
     1801 ataacttcat caacccaaaa aaaccgtacc cggttatcgc gaccacttca gagctgatga
     1861 ccaccggtgt ggatgctaaa acctgcaaac tggtagtact ggaccagaac atccagtcga
     1921 tgaccaaatt caagcaaatt atcggtcgtg gtacacgcat cgacgagcgt tacggcaaac
     1981 tctggtttac catcctcgac tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg
     2041 atggcattcc cgaaaaagtt atggatacca caccagagga tatcgccgat ccagaatctg
     2101 attttgaaga gaaactcgaa gaaatcagcg aacacgacga agagcaggtg acaggcgttg
     2161 atgaaccgcc tgcgccgcca taccaggtta ccgataccga tgatgttggc ccacttccgg
     2221 aagaagacga gaagaaaatc cgcaagtttc acgtcaatgg cgtggcggtg ggtgttattg
     2281 cgcagcgtgt tcagtattac gatgccgacg gtaaactggt taccgaatct tttaaagatt
     2341 acacccgcaa aacactgctc aaagaatatg cctcgctgaa tgactttacc cgcaagtggc
     2401 aggacgccga tcgcaaagaa gcgatcattc acgagctgga gcaacagggg atcatctggg
     2461 aagtactggc agaagaagtc ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag
     2521 tgtatggtca gccgccgtta acccgcaaag agcgcgccga gaacgtgcgc aagcggaact
     2581 acttcacaaa atactctgaa gcagcgcaag ccgtgctcga taatctgctg gataaatacg
     2641 ccgatgcggg cgtacaggag atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca
     2701 gcatgggcac cttaccggag attattaaaa ccggatttgg cgaccgtaac gggtataatc
     2761 aggcgctcag cgagctggaa aacgaaatct accaattacc gccccgctct gcttaacaac
     2821 tctcttttgc gccttctgca accatcgtgt tgcagaaggc cttgtttttc atggaataca
     2881 atatgtctat cagttcagta atcaaatcat tacaagatat tatgcgcaaa gatgccggtg
     2941 tggacggcga tgcgcagcgt ctcggtcagc tctcctggct actgtttttg aaaatcttcg
     3001 atgcccagga agaggcgctg gaactggagc aggataacta tcaatatccg atcccacagc
     3061 gttatttatg gcgcagttgg gccgcaaacg ctcagggcat taccggtgat tccctgctgg
     3121 aattcgttaa tgatgatctg ttcccggcgt taaaaaacct cactgcgcct atcgataaaa
     3181 acccacgcgg ctacgtggta aagcaggcgt tcagcgatgc ctataactac atgaaaaacg
     3241 gtacgctact gcgtcaggtg atcaacaagc tgaacgaaat tgactttacc agcgccagcg
     3301 aacggcatct gtttggtgat atttacgaac agatccttaa agatctgcaa tctgcgggca
     3361 atgcgggcga attctatact ccacgcgccg tcactcgctt tatggtggat cgcgttgatc
     3421 cgaaactcgg cgaatccatt atggacccgg cctgcggtac gggcggtttt cttgcctgcg
     3481 catttgatca tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag acgctgcaac
     3541 aacagatcca cggtgttgag aaaaaacagc ttccgcacct gctggcgacc accaatatgc
     3601 tgctacacgg cattgaagtg ccagtgcaaa ttcgtcacga caacactctg aacaaaccgc
     3661 tttcctcctg ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg
     3721 aagaagacgg tattgagaag aactttccag cagagatgca aacccgcgaa acggcggatt
     3781 tgttcctgca actgattgtg gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc
     3841 cggatggcac gctatttggc gaaggcgtta aaaccaaaat caaaaagctg cttaccgaag
     3901 agtgcaatct gcataccatc gtgcgtttac cgaatggtgt gtttaacccc tataccggca
     3961 ttaaaaccaa cctgttgttc tttaccaaag gtcagccaac caaagagatt tggttctatg
     4021 agcatccgta tccggcgggc gtgaaaaatt acagtaaaac caagccgatg aagtttgaag
     4081 agtttcaggc ggagatcgac tggtggggta acgaggccga tggttttgcc agccgcgtcg
     4141 agaatgagca ggcgtggaaa gtcagcattg atgatgtcat tgcgcgcaac ttcaatctgg
     4201 atattaaaaa cccacatcag gcggaaaccg ttagccatga tccggacgaa ctgttagcgc
     4261 agtatgcaaa acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc gatattcttg
     4321 gcgctgcgct gtctggcaag gaggttaact aatgagtgtg gaaaagctga tcgttgatca
     4381 tatggaaacc tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg gcagttccgg
     4441 aaaaattgat ttatatggca ttaagaaatt acgtgagctg attctggaac tggctgtgcg
     4501 cggtaaactg gtgccgcagg atccgaatga tgaaccggcg tcggagctgc tgaagcgtat
     4561 tgcggcggaa aaagcagagc tggtgaaaca ggggaaaatt aaaaagcaaa aaccactgcc
     4621 ggaaattagc gaggaagaga agccatttga attgccggag gggtgggagt gggtgcgatt
     4681 gggtgacttt accaatatca ttcgtggcat aacttttcca ggaaatgaga agtctcaatt
     4741 tcaagcccca ggtaaaatag cttgtttacg aacagcgaac gtgcaagaac aaatagattg
     4801 ggatgattta atatatatat ctgatagttt tgtaaagcga gatgatcaat acctccaaga
     4861 acacgatata gtcatgtcta tggcaaatag tcgtgaattg gttggtaaag tcgccctagc
     4921 ttctctacct gataatagca aatttacatt tggcggattc ttgagtgtat tgagaccatt
     4981 agttgttaat gaaatttatt taatggcctt actcagatgc gaaacttata aatcacagtt
     5041 aattgaatca gcaagtcaaa ctacaaatat tgctaatata tcgttagcaa aacttaaccc
     5101 attaccagta tgtattcctc cagctaaaga gcaaattcat attgttaaaa agatgaatga
     5161 actcatgtcc ctatgcgacc aactagaaca gcaatccctg accagtctgg acgcacatca
     5221 gcaactggtt gaaacactgt tgggaacgct tacacacagc caaaacgccg aggaactggc
     5281 tgaaaactgg gcgcgtatta gcgagcattt cgacacacca tttaccaccg aagccagcgt
     5341 ggatgcgtta aaacagacca ttctgcaact ggccgtaatg ggtaaacttg tgctgcagga
     5401 tccgaatgac gaaccagcct ctgaactgct caaacgaatt gcgcaggaaa aagctcaatt
     5461 ggtgaaagaa gggaaaataa aaaaacaaaa accgttgccc ccgattagcg atgaggaaaa
     5521 accgtttgaa cttccggaag ggtgggagtg gtgtcgttta aatgatattt catctaaaat
     5581 tactgatgga gatcataaaa caccacctcg tattgccgaa gggtataaat tactttcagc
     5641 aaaaaatatc cgtgacggtt atttagacta caataactgt gattatatct cagcgattga
     5701 ctatgaaaaa tcccgtgagc gatgcttacc tgaaaaaggt gacttattaa ttgtttcggt
     5761 tggtggaaca attggtagat cttctttaat taaggactgt tctgatttcg cactcgtaag
     5821 gagcgtagcg attataaaac cattattaat cgaacctgag tatttaaaac ttgcaatgga
     5881 ctcgaagtta ttacagtcaa tgattcactc tcataaacga ggcggagctc aaccatgcct
     5941 atatcttgga gaaattagta aatttctatt ccctacccct ccattggcgg aacaaaggaa
     6001 tatagttaat aaagttagta tccttatgga aaaatgtaga tttttatttt taggacttca
     6061 atccgcccaa c
//
LOCUS       DAZFRE010000184         5837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFRE010000184 DAZFRE010000000
VERSION     DAZFRE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.187"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1446
                     /gene="ydfI"
                     /locus_tag="ACHKUI_004886"
     CDS             <1..1446
                     /gene="ydfI"
                     /locus_tag="ACHKUI_004886"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6600904.1"
                     /translation="LSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHF
                     SDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLET
                     VLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATGMIVEA
                     LARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVD
                     RIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRKWVIEDNFVAGRPEWEKAGAELVSDV
                     LPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQ
                     GVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLAL
                     GVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLP
                     GNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1482..1685)
                     /gene="ydfZ"
                     /locus_tag="ACHKUI_004887"
     CDS             complement(1482..1685)
                     /gene="ydfZ"
                     /locus_tag="ACHKUI_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6600905.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1863..2549)
                     /gene="rspR"
                     /locus_tag="ACHKUI_004888"
                     /gene_synonym="ydfH"
     CDS             complement(1863..2549)
                     /gene="rspR"
                     /locus_tag="ACHKUI_004888"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6600906.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATIDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="ACHKUI_004889"
     CDS             complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="ACHKUI_004889"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6600907.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3521..5566
                     /gene="dcp"
                     /locus_tag="ACHKUI_004890"
     CDS             3521..5566
                     /gene="dcp"
                     /locus_tag="ACHKUI_004890"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6600908.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDVAQLAGMSEQEIAQAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWMRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSRAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(5611..5691)
                     /locus_tag="ACHKUI_004891"
                     /pseudo
     CDS             complement(5611..5691)
                     /locus_tag="ACHKUI_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5715..5819
                     /locus_tag="ACHKUI_004892"
                     /pseudo
     CDS             5715..5819
                     /locus_tag="ACHKUI_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT         1468 a         1392 c         1505 g         1472 t
ORIGIN      
        1 ttatcagcaa aagcgacgct ccctgtttat gatcgtaata acctggcccc aagaattgtt
       61 catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga tattcttgct
      121 acggaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg cgaacagcaa
      181 attgccgatt tacaacagca agataatctt tataccgttg cggaaatgtc ggctgatgcg
      241 tggacggctc gcgtcgttgg cgtcgttaaa aaagccttac acgtacaaat agatggctta
      301 gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct gacaatcacc
      361 gaaaaagggt atttccactc tccggcgacc ggacagttaa tgcttgatca cccgatggtc
      421 gttgccgacg tgcaaaatcc ccaccaaccg aaaactgcaa cagggatgat tgttgaggcg
      481 ctggctcgcc gtaaagcggc aggacttccc gcatttaccg tcatgtcatg tgacaacatg
      541 ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcct atgcgcaagc cattgatgta
      601 aaactggcac aatggatcga agataacgtg actttcccat caacaatggt ggaccgtatt
      661 gtgcccgcag tgacagagga tacgctggcg aaaatcgaac aacttaccgg tgtgcgcgat
      721 ccggcaggcg ttgcctgtga acctttccgc aagtgggtaa ttgaagataa ctttgttgcc
      781 ggacgtccgg aatgggaaaa agcgggagcc gaactggtta gtgatgtgct gccttatgaa
      841 gagatgaagt tgcgcatgct caatggcagt cattcattcc tggcgtatct ggggtatctt
      901 gccggatatc agcacattaa tgactgcatg gaagatgaac attatcgtca tgcagcgtat
      961 gccttaatgt tgcaggaaca agcgccgacg ctgaaagtgc agggcgttga tttgcaagat
     1021 tacgctaacc gattaattga acgctatagc aacccggcgt tacgtcatcg aacctggcag
     1081 attgcgatgg atggcagcca gaaattgcca cagcggatgt tggattctgt tcgctggcat
     1141 ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg gatgcgttat
     1201 gtcggtggtg ttgatgaaca gggaaatccg atagaaatca gtgatccact gttacctgtt
     1261 attcagaagg cagtacaaag tagtgctgaa gggacagcgc gcgtccagtc attgctggca
     1321 attaaggcga tctttggtga tgatttacca ggcaatagtt tgtttacgac aaaagtaacg
     1381 gaagcgtact tgtctttatt agcgcatggc gcgaaagcga ccgtggcgaa atattccgtg
     1441 aagtaaaaaa agcaatgccg ccatcggcgg cattcacacg cttagttcat gccgagacga
     1501 atcaggtcca gtggtgccag tttctcttca caaccttcaa caactacggt ttttccgcgg
     1561 cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc agtgtgaccg
     1621 gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg atcgtaggtc
     1681 gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg gcgcaatata
     1741 aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct tttttgatgc
     1801 atgcatgagc cataagtcgg ggggaaaaga ctctcacccc ggatgggatg agaggaaatg
     1861 aattactctt cgctaaacca gtcgctgttt tcctggcgga tctggcgcac ggattcgctg
     1921 atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg tttttgcagg
     1981 gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac gtggtcgaaa
     2041 ctcatatagc gcacgcgatc aatggtcgct ttcaggttct caatggtatc ccacgccagt
     2101 tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc aaaaaaatca
     2161 tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc cagttggtag
     2221 cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc aatggcctga
     2281 cggataaaac tgccgttgcg cacctgggcc atggaaattt tgttgacgta gctgccacgc
     2341 tgcggacgaa tttgaatcag accgttttcc gccagtttaa taaaggcttc acgaaccggc
     2401 tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt gcctggtgca
     2461 atcaggcaat ggacaatgtc gcggcgaaga atacgataaa tctgctgatt gacaggctgt
     2521 gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag ttaactgcat
     2581 gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt ataaaaatta
     2641 ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt ccagggtatt
     2701 gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga catcttctgg
     2761 tgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg
     2821 gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga cgcgcaccgc
     2881 cgtaccatgc aggtccgtac gcagattcag gctaaattga cgaacaaacg ctttcgtcgc
     2941 accgtaaacg ttaccaccgg catacggcca gctaccagcc gttgagccaa tgttaataat
     3001 atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat ataccaggcc
     3061 tttgttgttg gtatcaatca tcgtttccca gtcttcaatg ctggctttat gcgcaggctc
     3121 catgcccaac gccaggccgg cattgtttac caggatatca atattgctcc actcggcagg
     3181 gagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt gggcgatata
     3241 cagattatct cccagttcgt cttttaactc ctgtaaccgc tcctggcgac ggccagtggc
     3301 gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa aacctgccgt
     3361 tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt gtcaataaca
     3421 tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc cgttatcagt
     3481 aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt
     3541 ccttgtgcaa agcacactgc cgtatctggc tccccatttt gatcaaattg ccaaccatca
     3601 ctatcgcccg gcattcgatg agggaataca gcaaaagcgg gcagaaatag ctgccatcgc
     3661 gcttaacccg caaacacctg atttcaacaa tactattctg gcactggaac aaagcggaga
     3721 attacttacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata ccaatgatga
     3781 attacagcgt cttgatgaac agttttccgc tgaactggcg gaacttgcga atgatatcta
     3841 tctgaacggg gaattattcg cgcgggtaga tgctgtctgg caacgccgtg aatccctggg
     3901 gcttgatagt gaatccatcc gcctggtgga ggtgattcat caacgttttg tccttgccgg
     3961 agccaaactt gagcaagctg ataaagcaaa attaaaagta ctgaatacag aagctgcgac
     4021 cctgaccagc cagtttaacc agcggttact ggcagcaaat aaatccggcg gtctggttgt
     4081 gaacgatgtc gcgcagctgg caggaatgag tgaacaagag attgcgcagg cggcagaggc
     4141 ggctcgcgag aaaggtctgg ataacaactg gctgattccg ttgctgaata ccacccaaca
     4201 accagcgctt gctgaactgc gcgatcgcgc gacgcgtgaa aaactgttta ctgcgggctg
     4261 gatgcgagcg gaaaaaaatg atgccaatga tacccgcgct atcattcaac gtctggtgga
     4321 gatccgcgcg cagcaggcga aactgcttgg ttttcctcat tatgccgcat ggaaaatcgc
     4381 cgatcagatg gcaaaaacgc cagaagcagc gctcaacttt atgcgggaaa ttgttccagc
     4441 ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttatcg ataagcagca
     4501 gggcgggttt agcgcgcagc cgtgggactg ggcattttat gccgagcagg taagacgtga
     4561 gaaattcgat ctcgatgaat cgcagctcaa gccatatttt gaattaaaca cggtgttgaa
     4621 tgaaggcgta ttctggactg cgaatcagct cttcggtatt aagtttgtcg aacgttttga
     4681 tattcctgtc taccatcctg acgttcgtgt gtgggaaatt tttgatcata atggcgtggg
     4741 gctggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg gcgcatggat
     4801 gggcaatttt gttgagcaat caacgctgaa tgaaacgcac ccggtaattt ataacgtctg
     4861 caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg atgtaataac
     4921 cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc gttatgccac
     4981 gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca
     5041 ctgggcaaca catccgcagg tattcgctcg ctacgcccgg cattatcaga gcagagccgc
     5101 aatgcctgac gaacttcaac agaaaatgcg taatgccagc ctgttcaata aagggtatga
     5161 gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgtc tggaagaaaa
     5221 cgaagcaatg caggacgtcg atgatttcga attgcgggcg ctggtggcgg aaaatatgga
     5281 tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt ttggtggcgg
     5341 atatgccgca ggttattacg cttatctgtg gacgcaaatg ttggccgatg atggttacca
     5401 gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg cggcgttttc gcgaggcgat
     5461 tctttccaga ggtaacagcg aagatctgga acgcctgtat cgacaatggc gcggtaaggc
     5521 acctcagatt atgccgatgc tgcaacatcg tggcttgaac gtataaacac caccaatggc
     5581 tggcctggat ttatcgccag accagcttcg ttatagcagt aattgcagca actgggcgag
     5641 ggcaaacttc accgctaatg cgaatgccgt ttcgcagtcg ccagcaaaat tacttttttt
     5701 gtatactctt ccgttataat aattttcaaa ttcagccgga ccagatgatc aacattagcg
     5761 gcagtctgga taagaaaagc gcgccggccg tcgttcgggt cactcagttg cagaaataaa
     5821 aataaattag cctgatg
//
LOCUS       DAZFRE010000185         5765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFRE010000185 DAZFRE010000000
VERSION     DAZFRE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.188"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(138..386)
                     /locus_tag="ACHKUI_004893"
     CDS             complement(138..386)
                     /locus_tag="ACHKUI_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6600909.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(649..744)
                     /locus_tag="ACHKUI_004894"
                     /pseudo
     CDS             complement(649..744)
                     /locus_tag="ACHKUI_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(741..1307)
                     /gene="yecD"
                     /locus_tag="ACHKUI_004895"
     CDS             complement(741..1307)
                     /gene="yecD"
                     /locus_tag="ACHKUI_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6600910.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1617..3389
                     /gene="aspS"
                     /locus_tag="ACHKUI_004896"
     CDS             1617..3389
                     /gene="aspS"
                     /locus_tag="ACHKUI_004896"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6600911.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            3382..3834
                     /gene="nudB"
                     /locus_tag="ACHKUI_004897"
     CDS             3382..3834
                     /gene="nudB"
                     /locus_tag="ACHKUI_004897"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6600912.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            3863..4603
                     /gene="yebC"
                     /locus_tag="ACHKUI_004898"
     CDS             3863..4603
                     /gene="yebC"
                     /locus_tag="ACHKUI_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6600913.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4638..5159
                     /gene="ruvC"
                     /locus_tag="ACHKUI_004899"
     CDS             4638..5159
                     /gene="ruvC"
                     /locus_tag="ACHKUI_004899"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6600914.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5161..5763)
                     /gene="yebB"
                     /locus_tag="ACHKUI_004900"
     CDS             complement(5161..5763)
                     /gene="yebB"
                     /locus_tag="ACHKUI_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6600915.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
BASE COUNT         1439 a         1389 c         1536 g         1401 t
ORIGIN      
        1 tctggatgtt gatccgttaa atataaaaag taaaaagccg cagtaacttg tcatgacagg
       61 atactgcggc tggctggtta actttcgata gtgcgagtat tgaatgattt ccagctgtta
      121 ctgattttac gtgctaatta gtgaacaaac cactcgtcag cagactccca ggtatctttc
      181 agagtttcct gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg
      241 ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt
      301 cgggttaatt cttcctttaa cgcatccaca gcaccggttg gcattttagt cattttttct
      361 ttggctatgc agatttcaat acgcataaaa gtccctctat actgtgtttg tatacagtgt
      421 tatttttaac tgtatagata aacagtgtca agggtgaggg aaagtgattt tttggggcat
      481 gcatggggca aaaatatttg ttgtggggca ttttggggca tgattgggac actttgttac
      541 atatgaactt tgccgatttt catatgacat ggcaatccaa cgctttgatt attctaaaga
      601 aaacacatgc tcttgggcgt tctttagtga tttttaaaaa tcccgtatca cgcagttaaa
      661 gtggcgggca tactcttcaa ggctggtgat ccccaaccgc acccatttag gatgcgacca
      721 ttgcggcaga ccgatgtaaa tcataacgcg tggaggatct cttccacact gcgcacacgg
      781 gcgatgcgcg ggtagatatg attaatgctg ttattgtgct gctcggcgct agcggcgcta
      841 caggcatctt cggcaatcac cagattaaaa ccgagttccc aggcattgcg ggcggtggat
      901 tcaacaccga tattggtcga gatcccacat aacactattg tatcgatacc ccggcggcgt
      961 aattgcaact ccagatccgt accgtaaaac gcaccccatt gacgtttgat gatttcgata
     1021 tcgctgtcgg ttgcacctaa tgcagcagga tgttgccacc agttttcggg caacactttt
     1081 gcgggggagg gggcatcaac cggctgtttt aatgcttcgg cgtaatcggc agaccagcca
     1141 acgcgcacca gaaacacggg ctgaccgctg gcgcgaaatt tcgccgccag cttcccggcg
     1201 cgattaacca cctcatcggc agtatgtgga cctccggcaa aaggtaagat gccttcttgt
     1261 aaatcaatca ccaccagcgc ggtggtttta gcattaagtt caagcatgat gactcctgac
     1321 aaaacaaaat cgtgcctctc accttaatgc gattagccga tggcggaacg acaaattttg
     1381 ttaattttta tgagttcggg caacaccttt atagcaaact ggcgtagagt ctgatttcag
     1441 gcccgatata aagtggtaac gaggcgttcc agcgcgggcc aggctcttac ggtgcggcag
     1501 aatttccagt ataatagccg ccttttttca ttcagttgtg acatacagct aacgctgcga
     1561 cttggtcacc tgcgcagcag ggggcatccg ccggcagcta agttaaggga tatctcatgc
     1621 gtacagaata ttgtggacag ctccgtttgt cccacgtggg gcagcaggtg actctgtgtg
     1681 gttgggtcaa ccgtcgtcgt gatcttggta gcctgatctt catcgatatg cgcgaccgcg
     1741 aaggtatcgt gcaggtattt ttcgatccgg atcgtgcgga cgcgttaaag ctggcctctg
     1801 aactgcgtaa tgagttctgc attcaggtca cgggcaccgt acgtgcgcgt gacgaaaaaa
     1861 atattaaccg cgatatggcg actggcgaaa tcgaagtact ggcgtcctcg ctgactatca
     1921 tcaaccgtgc agatgttctg ccgcttgact ctaaccacgt caacaccgaa gaagcgcgtc
     1981 tgaaataccg ctacctcgac ctgcgtcgcc cggaaatggc tcagcgcctg aaaacccgcg
     2041 ctaaaatcac cagcctggtg cgccgtttta tggatgacca cggcttcctc gacatcgaaa
     2101 ctccgatgct gaccaaagcc acgcctgaag gcgcgcgtga ctacctggtg ccttctcgtg
     2161 tgcacaaagg taaattctac gcgctgccgc aatccccgca gttgttcaaa cagctactga
     2221 tgatgtccgg ctttgaccgc tactatcaga tcgttaaatg cttccgtgac gaagacctgc
     2281 gtgctgaccg tcagcctgaa tttactcaga tcgatgtgga aacttctttc atgaccgcgc
     2341 cgcaagtgcg tgaagtgatg gaagcgctgg tgcgtcatct gtggctggaa gtgaagggtg
     2401 tggatctggg cgatttcccg gtaatgacct ttgcagaagc agaacgccgt tatggttctg
     2461 ataaaccgga tctgcgtaac ccgatggaac tgaccgacgt tgccgatctg ctgaaatctg
     2521 ttgagtttgc agtatttgca ggtccggcga acgatccgaa aggccgtgtt gccgctctgc
     2581 gtgttccggg cggcgcatcg ttgacccgta agcagatcga cgaatacggt aacttcgtta
     2641 aaatctacgg cgcgaaaggt ctggcttaca tcaaagttaa cgaacgcgcg aaaggtctgg
     2701 aaggtatcaa cagcccggta gcgaagttcc tcaatgcaga aatcattgaa gccatcctgg
     2761 agcgtactgg cgcgcaagat ggcgatatga ttttcttcgg tgccgacaac aagaaaattg
     2821 ttgccgacgc gatgggggca ctgcgcctga aagtgggtaa agaccttggt ctgaccgacg
     2881 aaagcaaatg ggcaccgctg tgggttatcg acttcccgat gtttgaagac gacggtgaag
     2941 gcggcctgac ggcaatgcac catccgttca cctcaccgaa agacatgacg gcagcagaac
     3001 tgaaagctgc accggaaaat gcggtggcga acgcttacga tatggtcatc aatggttacg
     3061 aagtgggcgg tggttctgtg cgtatccata atggtgatat gcagcagacg gtgtttggta
     3121 ttctgggtat caacgaagag gaacagcgcg agaaattcgg cttcctgctc gacgccctga
     3181 aatacggtac tccgccgcac gcaggtctgg cattcggtct tgaccgtctg acgatgctgc
     3241 tgaccggcac cgacaatatc cgtgacgtta tcgccttccc gaaaaccacg gcggcggcgt
     3301 gtctgatgac tgaagcaccg agctttgcta acccgactgc actggctgag ctgagcattc
     3361 aggttgtgaa gaaggctgag aataactgat atggcttata agcgtcccgt ttcgatctta
     3421 gtggtcatct acgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc
     3481 gatttctggc agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc
     3541 atgcgcgaag taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt
     3601 gactgtcagc gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg
     3661 ggcgtgacgc gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcgccagatc
     3721 gttttcactg aacatctggc ttacaagtgg cttgatgcgt ctgctgcggc ggcgctcact
     3781 aagtcctgga gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc
     3841 agggctatct ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg
     3901 taaagctgcg caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt
     3961 aaccgcggct aagctgggcg gtggtgatcc ggacgctaac ccgcgtttgc gtgcagcaat
     4021 tgataaagca ctgtctaaca acatgacccg tgacactctg aaccgcgcaa ttgcacgagg
     4081 tgtgggcggt gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg
     4141 cggcacggca atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt
     4201 gcgtcatgca ttcagcaaat gtggcggtaa cctcggtact gatggttccg tagcttatct
     4261 gttcagcaaa aaaggcgtga tctccttcga gaaaggcgat gaagacacca tcatggaagc
     4321 agccctggaa gccggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta
     4381 cactgcatgg gaagaaatgg gcaaagtacg cgacgctctg gaagcggcag gtctgaaagc
     4441 agacagcgcg gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc
     4501 accgaaactg atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta
     4561 ccataacggt gaaatctctg atgaggttgc agcgactctc tgatgaggcc tgctaaacag
     4621 caaaacggag acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg
     4681 gctatggcgt catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac
     4741 gcaccaaagt ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa
     4801 tcatcaccca attccagcct gattatttcg ccattgaaca ggtctttatg gcgaagaacg
     4861 ctgactcagc cctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc
     4921 aggaattgcc ggtatttgaa tatgcggcac gtcaggtaaa gcaaacagtg gtaggtattg
     4981 gtagtgccga aaaaagccag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta
     5041 atccacaggc ggatgccgcc gatgcgctgg cgattgccat cacccactgc cacgttagtc
     5101 agaatgcgat gcaaatgagc gaatcgcgac tgaacctggc gagagggcga ctgcgttaag
     5161 ttataccgcc tcggtcagtt ccggctgagg cgtttcaact cccaccgcgt gaatcaacac
     5221 caaacccgga tgatgccaca aactggctgg cgtgacggtt ttacgctccc acggaataga
     5281 acctaagaac cagaatttcc agaaagtgag ttttgcattc ggattgctat ttaacaattc
     5341 tccaaacgtc tctatttcac ccaccggaat acatagcgcc tctttataaa tatcaaaaac
     5401 aaattttgaa cagaactggc gcgaagattc gtatttaaaa ccggtgtggt aaagtttgcg
     5461 tagccgggaa ggaacctgtt caacaattcg ttgtttttgt tgttctgtta gtccggcgtc
     5521 taatcgcttt atagcatagc gttgattagc agagcgttta ataaaacggg atagcgtagt
     5581 gatggttgag aggggaacac ggctttctgc aaccagaaag tcttcaccgt tatgaccgat
     5641 aatgatcccg acgtgattac tccagcaatt tgatgcagct gatatttgac caaataaggc
     5701 agcacctata catgtaaaga cgatatcacc aatttcatat tcagcaggat aattaatatt
     5761 cacgt
//
LOCUS       DAZFRE010000186         5553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFRE010000186 DAZFRE010000000
VERSION     DAZFRE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.189"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(141..2054)
                     /gene="mtlA"
                     /locus_tag="ACHKUI_004901"
     CDS             complement(141..2054)
                     /gene="mtlA"
                     /locus_tag="ACHKUI_004901"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6600916.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            2591..2953
                     /locus_tag="ACHKUI_004902"
     CDS             2591..2953
                     /locus_tag="ACHKUI_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6600917.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2956..4092
                     /locus_tag="ACHKUI_004903"
     CDS             2956..4092
                     /locus_tag="ACHKUI_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6600918.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDAVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(4236..5066)
                     /locus_tag="ACHKUI_004904"
     CDS             complement(4236..5066)
                     /locus_tag="ACHKUI_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6600919.1"
                     /translation="MNSRIACTTFDLPINTNLDNNHTLVFKKIRFYNSAVIFIRNAQL
                     VIKATDGDVIDISPGSLCYIEKNLVIDVTLKVLGSGVPYDIYTIDSDILSSVCKVMEP
                     ILSGQHLVTQVRKRIFFYKLEETDKEIFRRLISDDLPSYRQVYKIAYLLSRIHEIEPL
                     TYSLSVSTDITFTEKLKKIIEADLSRGWKLSDLRDILHMSEVSIRKKLERENNNFNAL
                     VLDIRMQHATKMITSTEKHINRISYDVGYTSTSYFIRNFKNYFGITPKQFSLKVKRKV
                     "
BASE COUNT         1485 a         1288 c         1278 g         1502 t
ORIGIN      
        1 attgtgtacc gactgccgga tgcggcgtaa acaccttatc cgaccgacaa aacaaattgt
       61 gctttatgtt gtaggcctga taagcgaagc gcatcaggca attagcccca ccttcttcat
      121 gcggagaggg tgggaatgga ttacttacga cctgccagca gttccagcac ttcatccacg
      181 ctggtggtgt gtgccagacg ctcgatgaca gactcatcat ccagtgcatt ggtcaggctg
      241 gtgataacct gaatgtgctc gttgttacgg gcagcaatac caatcaccag acgggcaatg
      301 tcatcttctt cttcaccgaa gcgcacgcct tccggatact ggcagaacac cacgcccgtt
      361 ttcagtacgc gatctttcgc ttcaaccgta ccgtgtggca ccgcgataga ctcacccaga
      421 taagtcgggg tcagtttttc acgatccagc atcgcctgaa cgtattccgg ctcaacgtaa
      481 ccgcctttca ctagctgctc gccagcaaaa cgaatcgctt cttctttggt ggccgctttg
      541 cgaccgagga agatgttctc cgcgcccagt ttgaacagat tagcactgga atcgtcaaag
      601 ctgtctttca gactgtcttt cactttcact tcgttttcag tatggcgctg ggcagcaacc
      661 agacgttcgg tcaggctggt gtacaggccg ctgtcgagga agttggtcag cgaaatatgc
      721 tgtgcctgcg gaacctggcg catagcgcgt tcggtcaggt cacggtgagt gatgaccagg
      781 tccacatctg gcggcaggtt gttgatcgcg ctgttagtga ccgaaatctg cgacagacct
      841 gcatcctgaa ttttcttacg cagcacacct gcgcccatcg cactggaacc cataccggcg
      901 tcacaggcaa cgatgatttt acgtacgtgg ctcaggtcgt tagtcacatc gcccgcagac
      961 agcggagacg cacctttaga ctcagctttc atgtcctgca tacgacgagt tgctgcttca
     1021 atatcatctt cttctttcac tttgctggtt ttcagcagaa tggcagagac aacgaaggag
     1081 acagccatcg ccgcacacac acccgcgatg ttagcgaagt atgcaccttt tggtgtcatc
     1141 gccagtacag caaggataga acccggagat gccggagaaa ccagcccacc gcccaggata
     1201 gtcagcgtga acacgccagt cataccaccg aggataacgg ccagaatcag acgcggattc
     1261 atcagcacat acgggaagta gatttcgtgg atacccccca ggaagtggat gattgccgca
     1321 ccgcccgcag actgtttagc gctaccacga ccaaagaaca tgtacgccag cagcacgccc
     1381 atacctggac ctgggttagc ttcaatcagg aagaagatgg atttacccag ttcatgggac
     1441 tgctgaatac ccagcggcga gaaaataccg tggttaatag cgttgttgag gaaaaggatt
     1501 ttcgccggtt caacaaagat agacgccagc ggcagcatgt catggacaac catgaagtta
     1561 acgcccgcag ccagcatttt agacagggct tcaacaaccg ggccaatgcc gagaaatgcc
     1621 agaatagcga ggatcatccc gatgatgcct gcggagaagt tattcaccag catttcaaaa
     1681 ccggatttga tcttaccgtc tacccagcgg tcgaagtgct taatgcacca gccgcccagc
     1741 ggacctgcaa tcatagagcc gaggaacatc ggcatatctg cgccgacgat aacgcccatg
     1801 gtggtgatgg caccgactac gccgccacgt tcgccgccta ccagcttacc accggtataa
     1861 ccgatcagca gcggcaggag ataagtgatc atcggcccga ccagcttcgc cagcgtttcg
     1921 ttcggtaacc accctgttgg aataaataac gcggtgatga taccccacgc gataaacgcg
     1981 ccgatatttg gcatcaccat gttgctgagg aaacgaccaa agctttgcac tttgatctta
     2041 atatcggatg acataaaaac accccttctt atgtttgctg tcgcgcaggc tggaagcccg
     2101 aggttttttg ttaatgtggc ggcagaggta gccggacctt gtagatactg cgaaatctgg
     2161 cactgaatcg gttaactgtc cagtcgacgg tcttttgtgt gatatcagtc acataaatat
     2221 agggggtaga gaggtaattg acgtgatgct catcacaaaa tgactgtgta aaaaagcaac
     2281 aagacggcga ttttcgctaa aaatggcatt taaatgtgag taatgtcaca tttttgtttt
     2341 gttagtttgt tgaatctttg tgatctgaat cacaagatat tttcttacgg cgttcacctg
     2401 atgtgatctg cagcatgtta tgccttcgtc ataaaatcgt tatgtaacta agcaactcat
     2461 cacctctaaa aattattctt tggtgttatg gcgagaatcg cgaacccgaa ttattgtcaa
     2521 aatatcagcc tgactccctt ctggctgcca gccaaaaaac aacaccgata aacgtgaatc
     2581 cgaggcagat atgtttctaa actatttcgc gttgggagtg ctgatcttcg tctttctggt
     2641 gattttttat ggaatcatcg cgatacatga catcccttat ctgattgcca aaaagcgcaa
     2701 ccatccccat gccgacgcta ttcatacggc gggctgggtg agcctgttta ctctgcatgt
     2761 tatctggccg tttctgtgga tctgggcgac gctctatcaa ccggagcgtg gctggggtat
     2821 gcagtcacat gttgcgtcgc aggagaaagc gactgacccg gaaatcgctg cactttctga
     2881 ccgaatttcc cggctggagc accaactcgc cgccgagaaa aagactgact attccacgtt
     2941 cccggagatc taaatatgga tctattgatt gttttaactt acgtggcgct ggcgtgggcg
     3001 gtctttaaaa tcttccgcat tccggtgaat cagtggacgc tggcgacggc ggcgctggga
     3061 ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact acaaccaccc ttacactttt
     3121 accgcgcaaa aggcagtgat agcgatcccc atcacgccac aggtgacggg aattgttact
     3181 gaagtcaccg acaaaaataa tcagcttatt caaaagggcg aggtgctttt taagctcgac
     3241 gcggttcgtt accaggcgcg agttgacaga cttcaggctg acctgatgac ggcgacgcat
     3301 aatataaaga cgctgcgcgc gcagctcact gaagcgcagg ccaacaccac ccaggtttca
     3361 gcggagcgcg accgtctgtt taaaaattat caacgttacc tgaaaggcag ccaggcggcg
     3421 gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc aaaatttcct cgcgcaggac
     3481 gcgctggtga aaggctcggt ggcggagcag gcgcagatcc agagccagct cgacagtatg
     3541 gttaacggcg agcagtcgca gattgtgagc ttaagagcgc aacttactga agcaaaatat
     3601 aaccttgagc agactgtcat tcgcgcgccg agcaatggct acgttactca ggtactgatc
     3661 cgcccaggta catacgcagc tgccttgccg ttgcgtccgg tgatggtttt catccccgag
     3721 caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct gaaacccggc
     3781 gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg tgttccacgg caaactgacc
     3841 agtattttac ctgtcgtacc aggcggttcg tatcaggcgc aaggggtatt gcaatcatta
     3901 acggtcgtgc ccggcacgga cggtgtgctg ggaaccattg aactggaccc caacgatgat
     3961 atcgatgctt tacccgacgg catctacgcc caggtggcag tttactccga ccatttcagc
     4021 catgtttcgg tgatgcgaaa agtgctgctg agaatgacca gttggatgca ttatttgtat
     4081 ttggatcatt gagggggaag gggcttggag gtacaggcgc tattggcaca gttctggctt
     4141 actggctatt gccagaactg gtagctaatc tcatgtcatt tgatgcactt actattaata
     4201 cacaggataa aaaaccattc tgtagaattt agatattaga cttttcgttt aaccttcaga
     4261 gaaaactgtt tcggtgtaat accaaagtaa tttttaaagt ttcgaatgaa ataagatgtg
     4321 cttgtatatc caacatcata cgaaattcta tttatatgtt tttcagtgga tgtaatcatt
     4381 ttcgtcgcat gctgcattct gatatctaaa actaacgcgt tgaagttatt attttctctt
     4441 tcgagttttt tcctaattga aacttctgac atatgtaaga tatctctcag atcgctcagt
     4501 ttccatcctc ttgataaatc tgcttcgatg atttttttga gtttctcagt aaaggtaata
     4561 tctgttgata cagacaagga atacgtaagt ggctcaattt catgaatcct tgaaagtaaa
     4621 taagcaatct tataaacttg cctgtatgat ggtaaatcat cactaataag tcgtctaaaa
     4681 atttctttat ctgtttcttc cagtttataa aaaaatattc tttttcttac ctgggtaact
     4741 aaatgttgcc ctgacaaaat cggttccatg accttacaaa cactacttaa tatatcgcta
     4801 tcaatagtat aaatatcata gggtacgcca cttccaagca cttttaatgt tacatcaatg
     4861 acaaggttct tttctatata acacaaagag ccgggagaga tatcaataac atctccatct
     4921 gttgccttaa tcactagttg tgcatttctg ataaaaatca ctgcactatt gtaaaaacgt
     4981 atttttttaa atacaagtgt gtgattgtta tccaagttag tattaatagg gaggtcaaac
     5041 gtggtacaag caattcgact attcataaag ctacctcatg ctttgtatat atgatttcat
     5101 gagtttttac gttgtgtaaa ctcgaaattt caacgtatga aaaaaaagtc taaacataaa
     5161 cattaataaa aaaacaatat gttatgctat gttaatcaag ctttattatg ataatagtaa
     5221 tattattgtt atgaaattat gcgtgattaa cgaagctata ttggttgcct atacgttgac
     5281 tgaaaatttt ctttaatgat gctggtaact ttctggtggt ttctattcct agttttgatt
     5341 taatacgatt gaatctatag taaattttac caattgaaag acatgttatt ttggatattt
     5401 cctttacact cttttgatgg aaaaaatata aaaaaaagat ctctctctca atgctattaa
     5461 atgagttaat tgatccaata gtagatgcat tattttcata aacgcatacg gttccattat
     5521 gtaatgtgat attctcaata caaatgcatt tta
//
LOCUS       DAZFRE010000187         5541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFRE010000187 DAZFRE010000000
VERSION     DAZFRE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.190"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(32..769)
                     /gene="bamD"
                     /locus_tag="ACHKUI_004905"
     CDS             complement(32..769)
                     /gene="bamD"
                     /locus_tag="ACHKUI_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6600920.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            904..1884
                     /gene="rluD"
                     /locus_tag="ACHKUI_004906"
     CDS             904..1884
                     /gene="rluD"
                     /locus_tag="ACHKUI_004906"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6600921.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1881..2612
                     /gene="yfiH"
                     /locus_tag="ACHKUI_004907"
     CDS             1881..2612
                     /gene="yfiH"
                     /locus_tag="ACHKUI_004907"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6600922.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMVVDAKSSTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2742..5315
                     /gene="clpB"
                     /locus_tag="ACHKUI_004908"
     CDS             2742..5315
                     /gene="clpB"
                     /locus_tag="ACHKUI_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6600923.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLDVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1373 a         1324 c         1499 g         1345 t
ORIGIN      
        1 caaggctgcc gttttgtgtt tcaggtttct gttatgtatt gctgctgttt gcggcgatga
       61 ttttcgctac tttttcagct tgcgcattca tctgcatctg acggtatgca ttttccatca
      121 gcggcagcgc atcacgcgta gcctgggtat ccggatagtc gcgcaacatg ccttctacgc
      181 ggttaacgac agcaacccat gcaccacgtt ctgtataata ctcggccacg gagtattcat
      241 atttcgccag acgatctttc aggaatacca gacgtttggt ggcatcggtg gtgtactgac
      301 tgttcggata gccgcgcacc agtttggaaa agtcactaaa cgcagctcgt gcatgttgag
      361 gatcgcgatc gctacggtca acgccaaaga acccttgcag cgcactgtca tccagcgcca
      421 tattggtcag gccacgcatg tacatgacat aatcgatatt cggatgggtc gggttaaggc
      481 gaataaaacg atcgatggca gcctgtgcta acggcaaatc ggcgttttta tagtaggcgt
      541 agatgagatc cagctgcacc tgctgcgaat acggaccaaa cggatagcga ttatctaacg
      601 cttccagttg cgttattgcc tgtctccagt taccgtcctg cagcttttgt tgtgcagtcg
      661 cgtaaatttc atttggcgga ttatcaggta cttcttcctt tgaccccgag caacccgcca
      721 aaaacaggct tagtgtggcg gctgccacca gatatttcat gcgcgtcatg acgttttgac
      781 tttcctcaaa atgtaatacg ggagattctc tgttcctgct cccggttaag accagctaca
      841 atagcacact atattaaacg gcaaagccgt aaaaccccaa cgataaacga agaagcagta
      901 tatatggcac aacgagtaca gctcactgca acggtgtccg aaaaccaact cggtcaacgc
      961 ttagatcagg ctttggccga aatgttcccg gattattcac gttcgcggat aaaagaatgg
     1021 atcctcgacc agcgtgtgct ggttaacggc aaagtgtgtg ataagccaaa agaaaaagta
     1081 ttgggtggcg agcaggttgc catcaacgct gagattgaag aagaagcgcg ttttgaaccg
     1141 caggatatcc cgctggatat cgtctatgaa gatgaagata tcatcgtcat taataaaccg
     1201 cgcgacctgg tggtacatcc tggcgcgggt aacccggatg gtacggtact gaatgcgttg
     1261 cttcattact atccgcccat tgccgatgta ccgcgtgcgg gcatcgtcca tcgtctggat
     1321 aaagacacca ccggcctgat ggttgtggca aaaaccgttc cggctcagac gcgtttagtc
     1381 gaatctttgc aacggcgtga aattactcgt gagtatgaag cggtggcaat tggtcatatg
     1441 accgctggcg gtacagtaga tgaaccaatc agccgccacc cgaccaaacg tactcacatg
     1501 gcggtgcatc cgatgggtaa accggcggtc actcactatc gcatcatgga acacttccgt
     1561 gtgcacacgc gtctgcggtt gcgtctggaa actggacgta cgcaccagat ccgcgtgcat
     1621 atggcccata tcactcatcc gctggtgggc gatccggttt atggtggccg tccgcgtccg
     1681 ccaaaaggtg cttcggaagc gtttatctcc acgctgcgta agtttgaccg ccaggcgctt
     1741 catgcaacca tgctgcgtct ttatcacccg atctccggta tcgaaatgga atggcatgcg
     1801 cctattccac aagatatggt ggagctgatt gaggtgatgc gcgccgattt cgaagagcat
     1861 aaggatgaag tggactggtt atgagtaagc tgattgtccc gcagtggccg ctgccaaaag
     1921 gtgttgcggc ctgtagctct acccgtatcg gcggcgtgag cttgccgcct tacgactctc
     1981 tcaaccttgg cgctcattgt ggtgataacc cggatcacgt tgaggagaat cgcaagcgac
     2041 tttttgctgc gggcaatttg ccttctaaac cggtctggct tgagcaggta cacggcaaag
     2101 atgtgcttaa gcttactggt gagccttatg cctcaaaacg agcagatgcc tcttatagca
     2161 atacgcccgg cacggtttgc gcagtgatga ctgccgattg cctccctgtg ctgttttgca
     2221 atcgtgcggg aacggaggtc gccgccgctc atgctggctg gcgtggactg tgcgcaggcg
     2281 tgttggaaga gacggtttcc tgttttgctg ataatccgga aaatattctc gcctggttag
     2341 ggccggcaat tgggccacgt gcgttcgaag tgggggcgga ggttcgcgag gcgtttatgg
     2401 tagtagacgc caaatcgagt acagctttca ttcagcatgg tgataagtat ctggcggata
     2461 tttatcagct tgcccggcag cgtctggcga gcgttggtgt tgaacaaatt tttggcggtg
     2521 accgttgtac atatacggaa aatgagactt tcttctctta tcgtcgcgac aagaccaccg
     2581 gtcgtatggc aagtttcatt tggctgatat aacctaaaga atcaagacga tccggtacgc
     2641 gtgattttct tttcacatta atctggtcaa taaccttgaa taattgaggg atgacctcat
     2701 ttaatctcca gtagcaactt tgatccgtta tgggaggagt tatgcgtctg gatcgtctta
     2761 ctaataaatt ccagcttgct cttgccgatg cccaatcact tgcactcggg cacgacaacc
     2821 aattcatcga accacttcat ttaatgagcg ccctgctgaa tcaggaaggg ggttcggtta
     2881 gtcctttatt aacatccgct ggcataaatg ctggccagtt gcgcacagat atcaatcagg
     2941 cattaaatcg tttaccgcag gttgaaggta ctggtggtga tgtccagcca tcacaggatc
     3001 tggtgcgcgt tcttaatctt tgcgacaagc tggcgcaaaa acgtggtgat aactttatct
     3061 cgtcagaact gttcgttctg gcggcacttg agtctcgcgg cacgctggcc gacatcctga
     3121 aagcagcagg ggcgaccacc gccaacatta ctcaagcgat tgaacaaatg cgtggaggtg
     3181 aaagcgtgaa cgatcaaggt gctgaagacc aacgtcaggc tttgaaaaaa tataccatcg
     3241 accttaccga acgagccgaa caaggcaaac tcgatccggt gattggtcgt gatgaagaaa
     3301 ttcgccgtac cattcaggtg ctgcaacgtc gtactaaaaa taacccggta ttgattggtg
     3361 aacccggcgt cggtaaaact gccatcgttg aaggtctggc gcagcgtatt atcaacggcg
     3421 aagtgcccga agggttgaaa ggccgccgcg tactggcgct ggatatgggc gcgctggtgg
     3481 ctggggcgaa atatcgcggt gagtttgaag aacgtttaaa aggcgtactt aacgatcttg
     3541 ctaaacagga aggcaacgtc atcctgttta tcgacgaatt acataccatg gtcggcgcgg
     3601 gtaaagccga tggcgcaatg gacgccggaa acatgctgaa acctgcactg gcgcgtggtg
     3661 aattgcactg cgtaggtgcc acgacgcttg acgaatatcg ccagtacatc gaaaaagatg
     3721 ctgcgctgga acgtcgtttc cagaaagtgt ttgttgccga gccttctgtt gaagatacca
     3781 ttgcgattct gcgtggcctg aaagaacgtt acgagctgca ccaccatgtg caaattactg
     3841 acccggcaat tgttgcagcg gcgacgttgt ctcatcgcta cattgctgac cgtcagctgc
     3901 cagataaagc catcgacctg atcgatgagg cagcatccag cattcgtatg cagattgact
     3961 caaaaccaga agaactcgac cgactcgatc gccgtatcat ccagctcaaa ctggaacaac
     4021 aggcgttaat gaaagagtct gatgaagcca gtaaaaaacg cctggatatg ctcaacgaag
     4081 aactgagcga caaagaacgt cagtactccg agttagaaga agagtggaaa gcagagaaag
     4141 cctcgctttc tggtacgcag accattaaag cggaactgga acaggcgaaa atcgctattg
     4201 aacaggctcg ccgtgtgggt gacctggcgc ggatgtctga actgcaatac ggcaaaatcc
     4261 cggaactgga aaagcaactg gaagccgcaa cgcagctcga aggcaaaact atgcgtctgt
     4321 tgcgtaataa agtgaccgat gccgaaattg ctgaagtgct ggcgcgttgg acgggaattc
     4381 cggtttctcg catgatggaa agcgagcgcg aaaaactgct gcgtatggag caagaactgc
     4441 accatcgcgt tattggtcag aacgaagcgg ttgatgcggt atctaacgct attcgtcgta
     4501 gccgtgcggg gctggcggat ccaaatcgcc cgattggttc attcctgttc ctcggcccaa
     4561 cgggtgtggg gaaaacagag ctttgtaagg cgctggcgaa ctttatgttt gatagcgacg
     4621 aggcgatggt ccgtattgat atgtccgagt ttatggagaa acactcggtg tctcgtctgg
     4681 ttggtgcgcc tccgggatat gttggttatg aagaaggtgg ctatctgacc gaagcggtgc
     4741 gtcgtcgtcc gtattccgtc atcctgctgg atgaagtgga aaaagcgcat ccggatgtct
     4801 tcaacattct gttgcaggta ctggatgatg ggcgtctgac tgacgggcag gggagaacgg
     4861 tcgacttccg taatacggtc gtcattatga cctctaacct cggttccgat ctgattcagg
     4921 aacgttttgg tgaactggat tatgcgcaca tgaaagagct ggtgctcgat gtggtaagcc
     4981 ataacttccg tccggaattc attaaccgta tcgatgaagt cgtcgtcttc catccgctgg
     5041 gtgaacaaca cattgcgtcg attgcgcaga ttcagttgaa acgtctgtac aaacgtctgg
     5101 aagaacgtgg ttatgaaatc cacatttctg acgaggcgct gaaactgctg agcgagaacg
     5161 gttacgatcc ggtctatggt gcacgtcctc tgaagcgtgc tatccagcag cagatcgaaa
     5221 acccgctggc acagcaaata ctgtctggtg aattggttcc gggtaaagtt attcgcctgg
     5281 aagttaatga agaccggatc gtcgccgtcc agtaatgata aaacgagcct tcggggctcg
     5341 tttttgtcta taagttagac ggaaaagact atattaaaga tgttttgcct gaaaaatgag
     5401 cgaacaataa agtttttata ttttttgctt gtcaggccgg aataactccc tataatgcgc
     5461 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
     5521 ctcaacagag gaaagcaaaa a
//
LOCUS       DAZFRE010000188         5529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFRE010000188 DAZFRE010000000
VERSION     DAZFRE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.191"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            209..1423
                     /gene="manD"
                     /locus_tag="ACHKUI_004909"
     CDS             209..1423
                     /gene="manD"
                     /locus_tag="ACHKUI_004909"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO6600924.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1490..2773
                     /locus_tag="ACHKUI_004910"
     CDS             1490..2773
                     /locus_tag="ACHKUI_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600925.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3025..3942
                     /gene="oxyR"
                     /locus_tag="ACHKUI_004911"
     CDS             3025..3942
                     /gene="oxyR"
                     /locus_tag="ACHKUI_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6600926.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3925..5325)
                     /gene="sthA"
                     /locus_tag="ACHKUI_004912"
     CDS             complement(3925..5325)
                     /gene="sthA"
                     /locus_tag="ACHKUI_004912"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6600927.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1237 a         1267 c         1504 g         1521 t
ORIGIN      
        1 taatggagtt tgttatgtga ttatttaacg tgttgtttta acgatgatgg ctaattattc
       61 aggtggtttt tgagggtgtt ttttctcctc ggtagaaatt tttattttta atgtgatcac
      121 ggttgaataa tcacttttgc tctcttgtat ggtagtaggc gtaaaattaa gtttgaagtg
      181 tctatttaca atacaggaga ttgcaataat gaaaattatt gctgtggatg tttttgttac
      241 ttgtcccggg cgtaatttcg ttacgcttaa aataaccact gaaagcggac tttgtggatt
      301 aggagatgcc acgttaaatg gtcgcgaatt gtcggtcgct tcttatctga aagatcatct
      361 ttgtcctcaa ttaattggcc gcgatgccag ccgtatcgaa gatatctggc aattctttta
      421 taaaggagct tactggcgac gtggtcccgt cacgatgtcg gccatttctg ccattgatat
      481 ggcgttgtgg gatatcaaag ctaaagcggc aaatatgcca ttgtatcaac tgcttggtgg
      541 cgcttctcgt gaaggcgtga tggtttattg ccacaccacc gggcggacga ttgatgaagt
      601 ccttgaagac tatgcaaagc accagcagat gggctttaag gcgattcgtg tgcagtgtgg
      661 tgtaccgggt atgcaaacca cctatggtct ggcgaagggc aaaggactgg cgtatgaacc
      721 agcaaccaaa ggcctgtggc cagaagagca gttgtggtct tcagaaaagt atctcgattt
      781 tacgccgaag ttatttgaag cggtgcgtaa taagttcggt tttaacgaac atctgttgca
      841 tgacatgcac catcgcctga cccccatcga agcggcacgc tttgggagga gcattgagga
      901 ttaccgcctg ttctggatgg aagatccgac gcctgccgag aaccaggaat gttttcgtct
      961 gattcgccag cataccgtga cgccgattgc ggtcggtgaa gtgtttaaca gtatctggga
     1021 ctgcaagcag ttaatcgaag agcagctgat tgactatatt cgcacgacga ttacccatgc
     1081 aggcggcatt accgggatgc gacggattgc cgattttgca tcgctttatc aggtacgtac
     1141 cggttcgcat ggcccttcag atttatcgcc tgtttgtcat gcggcggcgc tgcattttga
     1201 cctctgggtg ccaaactttg gcgttcagga gtacatgggc tattccgaac aaatgcttga
     1261 agtcttcccg cacagctggc gcttcgatga aggttatatg cacccaggcg atgaaccggg
     1321 gctgggtatt tctttcgatg agaaactggc ggcgaaatat ccgtatgacc cggcgtatct
     1381 ccccgtcgca cgactggaag atggcacatt gtggaactgg taatagcggt tcgcttaatt
     1441 attttctgaa tattcatcaa atcgattaat tccgctcaag gagctaaaaa tggacgttga
     1501 tgtgtcaacc agtgttgcgg gtaataaacc gcaacggatt cgtcgtattc agacagtgac
     1561 cctggtttta ttatttatgg cggggatcgt taattttctc gaccgttcgt cattgagcgt
     1621 ggcaggtgaa gcaattcgtg gtgagttagg attatccgct accgaatttg gtgttttgct
     1681 ttctgcattt tctctgtctt atggtttttc acaacttcct tccggtattt tgctagaccg
     1741 ctttggccca cgaatagtgt taggcgcagg cttaatattc tggtcattaa tgcaggcatt
     1801 aacaggaatg gttaattctt ttagccactt tattttaatg cgtatcggtc tgggtattgg
     1861 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cgcaaaaaga
     1921 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
     1981 tcctgctctt gtattgatgc aactggcatg gggctggcgg acgatgtttg tcatcatcgg
     2041 cgtagcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
     2101 tgtcctgacc gaggaagaac ggacatatct ctccgcgccg gttaaaccgc gtccacagtt
     2161 acaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
     2221 tttctctggc gtcaattata ccggttggct ctacatcgcg tggttacccg gttatttgca
     2281 ggccgaacaa ggtttcagtc tggcgaaaac cggttgggtg gcggcaattc ctttcctcgc
     2341 ggcggcggtc gggatgtggg ttaacggtat tgttgtcgat cggctggcga aaaaaggcta
     2401 tgacctggcg aagacgcgta aaacggcgat tgtctgcggt ttgatgatgt cggcattagg
     2461 cacgttgctg gtcgtgcaat cttcctcgcc agcccaggcg gtagcgttta tctcaatggc
     2521 gctgttctgc gtgcatttcg cgggaacatc tgcatggggg ctggtgcagg tgatggtgtc
     2581 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
     2641 ctttgctccg atcgtaaccg gttgggtcgt ggataccaca cactcgttta atctggcgct
     2701 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
     2761 tcggattgag taaccgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa
     2821 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
     2881 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt
     2941 atcagtcaga atgcttgata gggataatcg ttcgttgcta tgctacctat cgccgcgaac
     3001 tatcgtggca atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg
     3061 gccgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt
     3121 agcgggcaaa ttcgtaagct ggaagatgag ttgggcgtga tgttgctgga gcggaccagc
     3181 cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtactg
     3241 cgtgaggtga aagtccttaa agagatggca agccagcagg gcgagacgat gtccgggccg
     3301 ctgcatattg gtttgattcc cacggttgga ccgtacctgc taccgcatat tatcccgatg
     3361 ctgcaccaga cctttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
     3421 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc
     3481 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagac
     3541 cacccgtggg cgaaccgcga atgcgtaccg atggccgatc tggcagggga aaaactgctg
     3601 atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgttt tgaagccggg
     3661 gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg
     3721 gcaggtagcg ggatcacttt actgcctgcg ctggctgtgc cgccggaacg caaacgcgat
     3781 ggggttgttt atctgccgtg cattaagccg gaaccacgcc gcactattgg cctggtttat
     3841 cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga
     3901 atggatggcc atttcgataa agtgttaaaa caggcggttt aagccattta gcgcagctac
     3961 ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt
     4021 gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg
     4081 ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag
     4141 cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttaaact gggcgcggcc
     4201 cacttcatat ggcactttca tcgcggtcag ctgctgttcg gttttgccta cagagctgat
     4261 ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgt tggcttcacc
     4321 tttcaccagc gcctgcgcgg caatgcgccc ctggtcatag gctgccgacg ccaggctcgg
     4381 ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt
     4441 gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc
     4501 tgtattaccg gtgcgaccgt tggcatagag cagacagtca gctttcagtt ttttacccga
     4561 cttcagatgc atgatcacac cgtcgtcaca gccttcgatc ttctcgtact cttcgttgtg
     4621 acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca tctcttgatc
     4681 gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg
     4741 gaagatcgac gcatattcac agccgatcac tccagcaccg taaataagta catggcgcgg
     4801 ttcgtggtgc atgctaagaa ttgagtcgct gtcataaatg cgtggatggg tgaaatcaac
     4861 atctgttgga tgatatggac gagaaccgca ggcaataaca aatttttcag cagttagtgt
     4921 ttcgacgctg ccgtccgggc aatccagcgc caacgtatgc tcatcaacaa agcgagcgtt
     4981 cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg
     5041 attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcggga
     5101 atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg cgtgacggag
     5161 agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc
     5221 gataactgcg acgcgcgcac cttgcttaac caggcccatc gcagcgcctt cgccgccggg
     5281 gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttaccta
     5341 ttcttataca taaaagcaac agaatggtaa cattttatcg cgggtaagcc aattgatccc
     5401 cgtcatttat ctggctatat cgtgagcggc ctttgctttg tcttcctcct tcttatttta
     5461 tttattcact attaatcgct ttttcttatg gctgatagtt ttatcttgcc gaagcgtgct
     5521 tttttgtta
//
LOCUS       DAZFRE010000189         5511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFRE010000189 DAZFRE010000000
VERSION     DAZFRE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.192"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            604..829
                     /gene="ykgL"
                     /locus_tag="ACHKUI_004913"
                     /pseudo
     CDS             604..829
                     /gene="ykgL"
                     /locus_tag="ACHKUI_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(864..1004)
                     /gene="ykgO"
                     /locus_tag="ACHKUI_004914"
     CDS             complement(864..1004)
                     /gene="ykgO"
                     /locus_tag="ACHKUI_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6600928.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1004..1267)
                     /gene="ykgM"
                     /locus_tag="ACHKUI_004915"
     CDS             complement(1004..1267)
                     /gene="ykgM"
                     /locus_tag="ACHKUI_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6600929.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1632..1733)
                     /gene="ykgR"
                     /locus_tag="ACHKUI_004916"
     CDS             complement(1632..1733)
                     /gene="ykgR"
                     /locus_tag="ACHKUI_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO6600930.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2206..3348)
                     /locus_tag="ACHKUI_004917"
     CDS             complement(2206..3348)
                     /locus_tag="ACHKUI_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO6600931.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            complement(3521..4441)
                     /locus_tag="ACHKUI_004918"
     CDS             complement(3521..4441)
                     /locus_tag="ACHKUI_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6600932.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            4598..>5511
                     /locus_tag="ACHKUI_004919"
     CDS             4598..>5511
                     /locus_tag="ACHKUI_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6600933.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRV"
BASE COUNT         1552 a         1123 c         1190 g         1646 t
ORIGIN      
        1 atgaattcca tggttaagtc aatatttaca aaaaatgaaa atattgcttt tttgataaga
       61 agaaaaagac attcagtttc ataaggttat tggcaatata acgttcaatt cttacttgca
      121 ttttatatta gccaggaact ttgccctgag gtgttaggaa tagtcttaat gtgttgtgta
      181 atggactagt aaaaaaacaa gtttaaatga taagaaatag aatataaaac tatcataagt
      241 cataattaaa gatgatttgc caatcagtaa ggtgttttaa tggattaaca tgccgcacaa
      301 aatggttatg ggtgttcatc ccaataagtg atgaaaaatt aaacaggtgt aagtacgtca
      361 tctgtccggt aagcccccca agagccaaat taagtgaagt taaacgaaga ctgccagttg
      421 tcacaggaac gatggcctct ttgccagggc actgcattca gtcatctgta cgcgttactc
      481 taactggcac tcgaacgtcg gcgatggggt taccgatgga tctggccgtt cctgtggcgt
      541 aaacgttttt atgtcagtac caacagattt accacattta tcatcttaac agtttgagtg
      601 taaatgcagg ctgcgtcgca aagggaggaa actgagtggc gtgttcagtc taaaatagag
      661 ttgatgccag catacagggc gcagctggtc aacaagtcaa cattaatata atggaatatt
      721 cggagagaaa cgtcaggcag ttgaccttaa atgaacagga agatacatcc ccaggaaaat
      781 taatgctggt gtaataaata caccaacatt aatccggtct gattattaat cagacaaata
      841 tcgttaattc gtcgaatttt aaatcaacgt tttttcttac gaccctgaac ggccttaaaa
      901 cgtggattag atttacaaat cacatacagc cgtccttttc gcttcacaat ctgacagtct
      961 ggatggcgtt cttttgcggt acgcagagag ttaaggactt tcatcatgcc ccctttttcg
     1021 tgctaacaaa acgaccaaaa cgttgggtga accgtgcaac atttccttct gatgccactg
     1081 ttctcagctt ccctgtatag aacgggtgcg atttcgaaga gacatcaatt gttacgtacg
     1141 gatacgttac gccatccagc tcaatctcac ggtctgtttt gatagtcgag ccgattttaa
     1201 agtactcatc aacgctggtg tcgtggaaca ccacagtacg atactcagga tggatattgg
     1261 gtttcatcat ttttacctgt tatgttataa cataaccgta aagatacatg ctcatccgga
     1321 tcatttcaac acccaatcac tgtgactatt tgtgggatgt ggaatttccg caacggggaa
     1381 gtctggcatt gttagcagcc attggcgacg ctcccataaa cgcctggatg agtgattgat
     1441 gatggtttcg ctgagagtat ggatttttcg agaatggagg ctatctccac tttactggtc
     1501 gatgatttat tgcagcatga ttgattacct gacaatgtgt attcctctcc cacggatata
     1561 ataattactt acaaaaaagg gagaggatgc atattttaaa tatcactgaa gtgaacagtt
     1621 cattgccgtt attaatagaa atggagaaat aaataggcgt attctacaat tgcgacaaaa
     1681 acaacgatat taatcagttt atgactgatt tgctgtactt tattctcttt cattggtact
     1741 tcctcgcttt aaaaaagagt gcacttcgta agtgccctta tataaataac gagtttggtc
     1801 aaccaatttt ttgacatgta tcacaaattt gaatagatgt attacatcgg ctatctttta
     1861 ttgccccaac gtcattgata tatgtcgcct gaaatcagtt acgggaatga gtctgatctc
     1921 aagactggcc cagtcctggc gttgattggc gctgaggagc atatcgcatc tcatcgtaac
     1981 gtcgtatctc ctggggtgtt atacaagata ttgttgtagg taaccaggga gaggaattga
     2041 gtctattaaa ccgtcaacta tgccggatac atactggatt acactgcagg cacgccttgc
     2101 gagagaacgt gccgcagtga cgggttaatt atctgaaaga atttgcgagg ctgtatcggt
     2161 tactcattga tttgatagtt ttactctcgg gagagtaatc aatatttaat ccattaacgg
     2221 aaaccagcca gttcctttcg atgcctgaat ttgatcccat agtttatccg gaatggttaa
     2281 ttcaggaacg cgttgtggat taagggctgt ttgaatttca tgacttctac ctgattcagc
     2341 taaagtcacc cattctggat ttatcaccag ccctttaccg atcgcaacaa gtggcaggcc
     2401 agcggaaatt gcttcttgag cctgagacgg tgtgcgtatt ttacctgcgg cgattaccgg
     2461 cactctgcct gcaatatgat ttaaaatcaa ttcaatagtt cgggggccat ttggcgactc
     2521 aacagggtag ctatcgttaa tactgaccag cgaggtatgt atatacgaaa taccggatga
     2581 aatgagtcga tcaaggagtt tataagtatc ctctatccgc agcccccctg ttacagattc
     2641 ttcaggagat attctgtaac cgatagcaaa gggctttgtc gcatattcat agactacatt
     2701 ttttacttcc tgtaatacgg ccagcgggaa gcgcatacgt ccttcaagat ctcctcccca
     2761 tcggtcatta cgctggttaa atagagggga gaagaaattc tgcagaagga atccgtgcgc
     2821 accatgaagt tcaacgccat caaaacctgc tttaatcgcc ctttttgtga catctccgaa
     2881 tgctctaatg gtttcctgaa tttcgttttc agtcatttcc ctgctttgta ccactctctt
     2941 cataaagtca ccagatttta cgcttgatgc gctggcacta ataacatcgt tatttggcac
     3001 aagctccggg atggctttat taccggcgtg gaaaatctgc aaaatagccg gagcaccgcc
     3061 acttttggct gcagctgcta acttttcaag gctgttaatg aaacggtcgt catacgcggc
     3121 aaattcatga gtaaacccaa tgccgcttgg cgtgacatag gtacatcctg ttattactaa
     3181 accaacattt tgtgagcgac gtttataaaa ttcgagttct tgctcagaga tggttccatc
     3241 aggattagct gaccaggttg tcataggagc cataacgata cgattacgta gctttatttt
     3301 ttctgtcagc ataaaaggag aaaacaatga tgggtgttta tttgtcataa caatacctca
     3361 aatgtttatc tgattttaat gcgtataatc ttaataagtt gagttgctac tttttgatgc
     3421 ttttttcctt taatagaatt ctttttgagt aattttatta ttgtcgtgta gtcaataaag
     3481 gataaatgtc agggagataa tgaatctccc tgcaaggtta ttacaaatgt gttttgtaaa
     3541 aaggaattaa tttatcgagc gcctgtttca caggttctgg cttatcatac aggtcgtaat
     3601 gtgaataacc ttcaaccacg accagttctt tatgttttga ggctgcacga ccaataattt
     3661 cgcagccgtc acgataggca ccaaaagcgc ccactctatc gcccactaca accattagtg
     3721 gttgagttaa caggacttct gcaagatgaa aagcatccca gcctacggca acagtctgat
     3781 gtgaaaatag cgaacgattt accccattag gcgcacagcc tcgtggcgaa cgataatatt
     3841 ctgttgcttc aaaaaggtct atttctgtta atcccgcttc aagagctgct tccggagaag
     3901 aagggagcaa atcatctacg cgaagttttg cgccgttagc ttcgtctgtt ctttgttgcg
     3961 ccattgcttc gagtgctccg ataggattaa acgctgtaaa tccctcacgc ataagacgcc
     4021 cgtagtttgc acccgtgaca gtaccaatgg ctttaatccg acgttctgtc attgcggcat
     4081 taatggcata tccacctcca ccacaaatac ccagtacacc aatacgcccg gcatcaacgt
     4141 aaggtaaggt ggtaaggtaa tcaacgacaa tgctgaagtc ttttactcgc attgttggat
     4201 cttccagata acgcggttct ccaccgctgc ttccctgaaa actggcatca aaagcaatga
     4261 cgataaagcc agcttttgcc agggcggcac catacacact acccgatgtc tgttctttgc
     4321 agcttccaat tggatgggca ctgataatgg caggatactt tttttcacta ttaaaatcgt
     4381 cagggaaata gatatcagca gctatatccc agtaagtgtg ttttatcgaa acagttttca
     4441 tttcaagctc cagggatttg cctaaacaga tttaaatatc atttctatta tccgcaactg
     4501 gcattcgtgt tcatatcgcg aacggcgtaa tagtatgact tgtattggtt atcatcaatg
     4561 gtctaatatc tatcacagtg ttaagttaag gtttacaatg atgaaaatag agccttcaat
     4621 tttgccttct cttgcctggt ttgcgctgat tgttcgtgcc ggcagttttt ccagagcagc
     4681 ctctgaaatg ggtattacgc gggccgcatt gtcccagaat ttaaaatctc ttgaggaacg
     4741 tcttaacacc aagctgatat accgtacgac ccgcaatatg tcgcttactg aggaaggaca
     4801 gcatctgtat gaagtgctgg tgtcggcttt ggggcaaatt gatgatgcgt tgaaagatgt
     4861 cggtgatacc caacttgaac ctacaggact gttgagaata aattcctccc gtgttgcagc
     4921 aaggatgctt gttgagcctc atattggcga gtttcttact cgttacccta aaacaaagat
     4981 agaactcatt atggatgacg gtttatctaa cattattgcg gacggctgtg atgtgggcat
     5041 acgtcttgag caaggactgg atgagcatat gactgccgtt cctgtttctc cgcttattaa
     5101 actggttact gtggcatcgc cagattattt aaaagaacac ggtattcctg aaactcctca
     5161 ggaattgagt aaccataatt gtcttcggtt gcggcataaa agtagcggtg cactctcagc
     5221 atgggagttt tcgaatgttg tggggggtaa tgaagagttc gaaatagagg tgtcaggtaa
     5281 gtatatttct aatgatgacg aaagcatgat acgaatggcg ctgaatggaa cgggtatcat
     5341 tcaacatctg gattttgcaa tcgctgaaca tatcaatgcg ggcaagttac agccgattct
     5401 tgaggattgg gccgtttcat ttcctggatt ttatatatat gtttcatcac gggtgagaat
     5461 gccctctaaa gttcgtgctt ttattgattt tatggtggag aaaagagtaa a
//
LOCUS       DAZFRE010000190         5404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFRE010000190 DAZFRE010000000
VERSION     DAZFRE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.193"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            4..765
                     /gene="ydeO"
                     /locus_tag="ACHKUI_004920"
     CDS             4..765
                     /gene="ydeO"
                     /locus_tag="ACHKUI_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6600934.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMNALAI"
     gene            1167..2849
                     /gene="ydeN"
                     /locus_tag="ACHKUI_004921"
     CDS             1167..2849
                     /gene="ydeN"
                     /locus_tag="ACHKUI_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6600935.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2901..4058
                     /gene="ydeM"
                     /locus_tag="ACHKUI_004922"
     CDS             2901..4058
                     /gene="ydeM"
                     /locus_tag="ACHKUI_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6600936.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4024..4149)
                     /locus_tag="ACHKUI_004923"
     CDS             complement(4024..4149)
                     /locus_tag="ACHKUI_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600937.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            4169..5326
                     /locus_tag="ACHKUI_004924"
                     /pseudo
     CDS             4169..5326
                     /locus_tag="ACHKUI_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
BASE COUNT         1634 a         1154 c         1109 g         1507 t
ORIGIN      
        1 aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc taacatttta
       61 gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg
      121 cattttgaac cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa taccattaaa
      181 aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta
      241 cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa
      301 gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc
      361 gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc cgggaaagtg
      421 agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc
      481 ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca
      541 cagattcttt tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca
      601 gtcaataaaa ttgccgaaca atgtggttat gcgagtacat cttattttat ttatgcgttc
      661 cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaaggcac
      721 acgggtatga acacggacaa cacgatgaat gctttagcta tttgattatt tgctaacgag
      781 tagtcaacca cacacgctgc gtaagaatta aatggggcag ccattccctg ccccgcgttg
      841 tttttaggcg atatatttct tgaaataaat aagtgacagt catcacatat ttatgcattt
      901 gcataacctg ttgcatgatt atttatgatc tcagttcggc attttggcag taaaatacaa
      961 taatttatta aatatcaata gattagttgt ttatcggcga gaaattactt agtagaacag
     1021 aaagtaatgt caatgcgtta tggactgtta tttccctttt tttagctaaa tttgctatct
     1081 atttatgtga ctaacttcac ttacattaac ttatttctct tcgtaaaatt actttggaat
     1141 taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt gtcgtaagta
     1201 cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat gcggcagatg
     1261 atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agactttacg ccgactgaat
     1321 acagtaccaa aggaaagcca aatattatcg tactgaccat ggatgatctt ggttatggac
     1381 aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg
     1441 atacctacaa aatagggata gataaagcca ttgaagctgc gcaaaaatca acgccgacgc
     1501 tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg ctatgtggcg cacggtgttt
     1561 ccggcccgtc ccgcgccgca ataatgaccg gtcgagcgcc cgcccgcttt ggtgtctatt
     1621 ccaatactga tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc
     1681 agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg
     1741 tgccagtgcc ggaagataaa caaacgcgtg actatcatga caacttcact accttttctg
     1801 cggaagaatg gcaaccgcaa aaccgtggct ttgattactt tatgggattc catgctgcgg
     1861 gaacggcata ttacaactcc ccttcactgt tcaaaaaccg tgaacgcgtc cccgcaaaag
     1921 gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac
     1981 ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcatctgcca aatgataatc
     2041 ctgctccgga gcaatatcag aagcaattta ataccggcag tcaaacagca gataattact
     2101 acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa
     2161 acggacagta tgacaataca attattctct ttacctccga taatggtgca gttatcgatg
     2221 gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta
     2281 ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat tatgacaagc
     2341 tgatttccgc aatggatttc tacccgacag ctcttgatgc agccgatatc agcattccaa
     2401 aagaccttaa gctggatggc gtttccttgc tgccctggtt gcaaaataag aaacaaggcg
     2461 agccacataa aaatctgacc tggataacat cttattctca ctggtttgat gaggaaaaca
     2521 ttccattctg ggataattac cacaaatttg tccgccatca gtcagacgat tacccgcata
     2581 accccaacac cgaggactta agccaattct cttatacggt gagaaataac gattattcgc
     2641 tcgtctatac agtagaaaat aatcagttag gtctatacaa actgacggat ctacagcaaa
     2701 aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag
     2761 agtttatcga taacagccag ccaccgctta gcgaggtaaa tcaggagaag tttaacaata
     2821 tcaagaaagc attaagcgaa gcgaaataac taaaccttca tgcggcggat ttttccgccg
     2881 ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt caatgtaatc
     2941 tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat gaaaaatgga
     3001 tggatgacag cactctgaaa gagttcatca aacaatatat cgcagcgtct ggcaatcagg
     3061 tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt ttccgtaaag
     3121 ttattcacta tcaacaacgc tatgcaggcc aaaaacgaat ttttaatgca ttacaaacga
     3181 atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgag tttctggttg
     3241 gtatctcgat cgatggaccc caagaactac atgaccgcta cagacgcagt aattcaggta
     3301 acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat caaatagagt
     3361 ttaatacgtt aaccgtcatt aataacatta atgtccatta ccctcttgag gtttatcatt
     3421 ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctcgaa accgggacgc
     3481 cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat ttttctgtgc
     3541 ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg
     3601 tgggtgaaat tttcatccgt cagtttgaaa gctttgtcag ccgttttttg gggaatgggc
     3661 ataccagttg tattttccag gagtcctgca aggataatct ggttgttgaa agtaatggag
     3721 acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac attaataaat
     3781 cggaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg atttcagcga
     3841 aatgtcagca atgtgtatat aaacctatct gcaatggtgg ttgccctaag catcgtatta
     3901 ctaaagtaaa caatgagact gtttcctatt tttgcgaagg ttataaaatc cttttttcaa
     3961 ccatggtacc ttatatgaac gccatggtag agttagctaa gaacagagta ccgctttacc
     4021 acattatgga tgttgcaaaa caaatggaga ataattaata acgctccccc ttagctccat
     4081 tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg tcgggtgttt
     4141 cgtcttcatc atcttccttt gcgaaaatat tgccgtatct aataaaggcg atttaagttt
     4201 ccctgcgcag caaaaatcac ttatcgcgct ggctaataaa gcagtaagcg gaagcggcgt
     4261 tggtgaactc tcctcttcca gcctgaaacg ctggagtgcg gaaaactcgg taaccatgag
     4321 cagtaaagtc agtggcatga atacgttgct ctccgttagc gcgcggacta ataaccctga
     4381 acctggtttc cagttgatta accagcgaat cacccacagc acgattaacg acaatatttg
     4441 ggcatcgctg caaaatgctc aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga
     4501 gaaattcgcc cagcagatgt atgagacgcg ctatgccgat gaccgcacga aattaccgca
     4561 agaaaatcag attgcacagt ttactgccgc agatgcgctg gctgccgatc gccagttgtt
     4621 ttcatctcca gcggatatca cgtttgtcat tgtcggtaat gtcgcagaag acaaactcgt
     4681 ggcgttaatt acgcgttact taggatctat caaacactct gattcgccat tagccgcagg
     4741 taaaccatta actcgcgcga cggacaacgc atcggttact gtaaaagaac aaaatgaacc
     4801 agtggcacag gtttcacagt ggaagcgtta taattcccgg acacctgtta atctggcgac
     4861 gcgtatggcg ctcgatgctt ttaacgtcgc actggcaaaa gatctacgca ttaatattcg
     4921 tgaacaggca tctggggcat acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa
     4981 agatatcagt catttgctgg cttttacttg tcaaccagaa cgacatgatg aactgttaac
     5041 gttagcgaat gaagtgatgg ttaagcgtct ggccaaaggg atcagtgagc aagaactgaa
     5101 cgaataccag caaaacgttc agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt
     5161 agcgaacacc attgtaaata gtcttattca atatgacgat cctgcagcat ggattgagca
     5221 ggagcaattg ttgaaacaaa tgacggtaga gaatgttaac actgctgtta aacaatatct
     5281 ttctcatccg gtgaatacgt ataccggagt attattgcca aaataatcac agccccgtca
     5341 acacatcgtt ggcggggatt ttatcaatat tcgctatttt tatgtaataa ttttataaat
     5401 gtgt
//
LOCUS       DAZFRE010000191         5348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFRE010000191 DAZFRE010000000
VERSION     DAZFRE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.194"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            145..1941
                     /locus_tag="ACHKUI_004925"
     CDS             145..1941
                     /locus_tag="ACHKUI_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO6600938.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFTNKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1959..2627
                     /locus_tag="ACHKUI_004926"
     CDS             1959..2627
                     /locus_tag="ACHKUI_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO6600939.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2642..3313
                     /gene="dsbI"
                     /locus_tag="ACHKUI_004927"
     CDS             2642..3313
                     /gene="dsbI"
                     /locus_tag="ACHKUI_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO6600940.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3359..4147)
                     /gene="ygiD"
                     /locus_tag="ACHKUI_004928"
     CDS             complement(3359..4147)
                     /gene="ygiD"
                     /locus_tag="ACHKUI_004928"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6600941.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEDGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4290..5063
                     /gene="zupT"
                     /locus_tag="ACHKUI_004929"
     CDS             4290..5063
                     /gene="zupT"
                     /locus_tag="ACHKUI_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6600942.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(5121..5291)
                     /gene="yqiD"
                     /locus_tag="ACHKUI_004930"
     CDS             complement(5121..5291)
                     /gene="yqiD"
                     /locus_tag="ACHKUI_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6600943.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         1390 a         1317 c         1283 g         1358 t
ORIGIN      
        1 aacaacatat ttaaaagaaa cacaaaaaca ttctcactct ttattgaatt aatatttata
       61 ttcacgtaac gaatttatta gcaccctctc aacagtcgat attcatcatt attatatatt
      121 tgcgttatat aaggagatat atccatgttt gataaatata gaaaaacact cgtagccgga
      181 actgtggcga taaccctggg tttgtcagca tcgggggtga tggctgcggg ttttaaacct
      241 gcgccgcctg ccgggcaact aggtgcggtc attgtcgatc cctacggcaa tgcaccactg
      301 accgctttgg ttgacttaga tagccatgtt atttctgacg tcaaagttac cgtccatggg
      361 aagggcgaaa aaggcgtaga aatcagctat cccgtgggtc aggaatcact aaaaacttac
      421 gatggtgtac cgatttttgg tctttatcag aaatttacta acaaagtgac cgttgagtgg
      481 aaagaaaacg gcaaggtcat gaaagatgat tatgtggtgc acacttcggc catcgtcaat
      541 aattacatgg ataaccgctc tatctccgat ttacaacaga ccaaagttat taaagtcgca
      601 ccgggttttg aagatcgcct ctatctggtt aatacccaca cctttaccgc ccaaggttcc
      661 gatctccact ggcatggtga gaaagataaa aatgccggta tccttgatgc gggtccggca
      721 accggcgcac tcccttttga tatcgcgcca ttcaccttta tcgtcgatac cgaaggtgaa
      781 taccgctggt ggctggatca agacactttc tacgatggtc gtgaccgcga tatcaacaag
      841 cgtggttatc tgatgggtat ccgcgaaacg ccacgcggca cctttaccgc tgtacaaggt
      901 cagcactggt acgagttcga catgatgggg caggtgctcg aagatcacaa actgccgcgc
      961 ggatttgctg acgccactca tgagtccatt gagacgccaa atggcacggt actgttgcgc
     1021 gtaggtaaga gtaactatcg tcgcgatgac ggcgtacacg tcaccaccat tcgtgaccat
     1081 atcctcgaag tcgataaatc tggtcgcgtt gtcgatgtat gggatctgac caagatcctc
     1141 gatccgaaac gcgatgcact gctcggcgcg ctggatgcag gtgcagtttg cgttaacgtt
     1201 gaccttgccc atgcaggaca acaggcaaaa ctggaaccag atacaccgtt cggcgacgcg
     1261 ctgggtgtag ggccaggccg caactgggcg cacgtgaact ctatcgccta tgacgcaaaa
     1321 gatgactcta tcatcctctc ctctcgccac cagggtgttg tgaaaattgg tcgtgataag
     1381 caagtgaaat ggatccttgc gccctctaaa ggttgggaaa aaccgctggc cagcaagctg
     1441 ctgaaaccgg ttgatgctaa cggtaagcca attacctgta acgaaaatgg cctgtgcgaa
     1501 aactcagact tcgactttac ctacacccag cataccgcct ggatttccag caaaggaacg
     1561 ctcaccattt ttgataatgg cgatgggcgt catctggaac aacctgcctt accaaccatg
     1621 aaatactctc gctttgtgga atataagatt gatgagaaga aaggcaccgt tcagcaagtg
     1681 tgggaatacg gtaaagaacg tggctacgat ttctatagcc cgattacctc catcattgaa
     1741 tatcaagccg accgtaacac catgtttggc ttcggtggtt ctattcattt gttcgatgtc
     1801 gggcagccaa ccgtcggtaa gttgaacgaa atcgattaca aaaccaaaga agtgaaagtg
     1861 gaaatcgacg tgctgtcaga taaacccaat cagactcact accgtgcgct gttagtccgt
     1921 ccacaacaga tgttcaaata attaagaagg gaataaaaat gtcgaaatta gggatctcat
     1981 cactatttaa aaccatactg cttaccgcag ctttggctgt ttcatttacc gcttctgcat
     2041 ttaccgaagg cacggattac atggttctgg aaaaacctat tccgaatgcc gacaaaacgc
     2101 tgattaaggt atttagctac gcctgcccgt tctgttacaa gtacgacaaa gcggttaccg
     2161 gtccggtatc ggaaaaagtg aaagatatcg ttgctttcac gccgttccat ctggaaacca
     2221 aaggtgaata cggcaaacaa gcaagcgaag tctttgcggt cttgatcaat aaagataaag
     2281 cagcaggcat ttccttattt gatgctaact ctcagtttaa gaaagccaag tttgcctact
     2341 acgcggctta tcacgacaaa aaagaacgct ggtctgacgg taaagatccg gctgctttta
     2401 tcaaaaccgg tctggatgcc gcaggtatga gtcaggcgga ttttgaagcg gcactgaaag
     2461 aaccagcagt tcaagaaaca ctggaaaaat ggaaagcctc ttatgatgtc gccaaaatcc
     2521 agggggttcc agcctatgtc gttaatggta agtacttgat ctacaccaag agtatcaaat
     2581 ccatcgacgc gatggcagac cttatccgtg agttagcaag caaataacag ggaaacaggc
     2641 tatgggtatt aagggaatgt ggaaagatct tcgcacatca ccagttgaca cactggtcag
     2701 atggcaggag caacgcttgt tgtggttgtt aatggccgtt gcgatgggcg cgttaattat
     2761 cctggcgcac tctttcttcc agatctatct ctacatggca ccctgtgagc aatgtgtata
     2821 cattcgctac gccatgtttg tgatggttat tggaggattg gttgcagcga tcaacccaaa
     2881 gaacatcatc ctaaagctaa ttggctgcgt gatggcattt tacggcagca ttttgggact
     2941 gaagttttcg ctcaaactga acgatatcca ccatgcagta cataatccgg atcccgattc
     3001 actgtttggc gtacagggtt gctctaccga tcccactttt ccctttaacc tgccgctggc
     3061 acagtgggca ccaaattggt tcaagcctac cggggattgt ggctatgacg caccaatcgt
     3121 tcctgatggc gtcacactga gcagcacgca acagtggttt gtggaaatgt atcagcaatc
     3181 tgaaggctgg tatctgctac caccatggca ttttatgaat atggctcagg cttgtatgct
     3241 ggcttttggt atgtgtctgg tgctgctggt cattatgagt ggcgcatggg cgcttaaaat
     3301 catcagaggc taactcagta acacccgaag ccagtcgttc tggcttcggg ttcttttatt
     3361 aacctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
     3421 taattggctc ctgcccatcc cacgccccta agacatacaa caacggcaga tagtgctctg
     3481 gcgttgggtt cgataacgcg ccatcttcat ggtcgagata attcaccaga ggatgttgtt
     3541 ccaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg
     3601 gatacggtga actatcaccg tgccacttca ctgtacgcag gttatgtacc acgttgccac
     3661 tggcgaccaa cattatccct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat
     3721 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata tcggcgtcag
     3781 ggtacatctt aatcagtacg ccccacgagc cgtggtcgaa gccccaggct tctttatcca
     3841 gcgtcacagg gaccggcgct aacagctcaa ccagatgctg tgccagcgca ggtgaacccg
     3901 gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
     3961 gcggcatctc catcgcggtc actcctgttc cacgggtaaa ccaatgagcc gaaaccacca
     4021 ctattgcttg cgggcgtggc aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat
     4081 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
     4141 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt
     4201 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
     4261 tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
     4321 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc
     4381 gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc tcattaatgg
     4441 aaatgcttcc tgccgcatta gccgccgaag gaatgtcgcc tgtgttgggt tatggaatgt
     4501 ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccacat gcgcatccgc
     4561 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
     4621 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
     4681 cgagcagcaa cctggagctg ggatttggca tcgccctggc cgtagcgttg cacaatattc
     4741 ctgaaggtct ggcggttgca ggcccggttt acgcggcaac gggatctaaa cgcaccgcga
     4801 ttctgtgggc gggcatttcc gggctggcag aaattcttgg tggtgtgctg gcatggttaa
     4861 tcctcggtag catgatttct ccggtggtca tggcggcaat catggcggcg gttgcaggaa
     4921 ttatggtggc gctctccgtt gacgaattaa tgccgctcgc caaagaaatt gaccccaata
     4981 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc
     5041 tacaaacggt gggaattggt taaaagcaac atcagaagtt gtccagcgca tggacaactt
     5101 ctgtcagctt cactaaagtc ttattctacc ttttcggctg tctcttctct cgtactgttt
     5161 aagtatttgt tccgcgcttc aagcagggcg tctctgtcct ggcgaaacgc ccgacaatat
     5221 cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag
     5281 gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt
     5341 cacatatc
//
LOCUS       DAZFRE010000192         5075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFRE010000192 DAZFRE010000000
VERSION     DAZFRE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.196"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..423)
                     /locus_tag="ACHKUI_004931"
     CDS             complement(<1..423)
                     /locus_tag="ACHKUI_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6600944.1"
                     /translation="MTTVSHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYY"
     gene            complement(515..1333)
                     /locus_tag="ACHKUI_004932"
     CDS             complement(515..1333)
                     /locus_tag="ACHKUI_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6600945.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMC"
     gene            complement(1423..1656)
                     /locus_tag="ACHKUI_004933"
     CDS             complement(1423..1656)
                     /locus_tag="ACHKUI_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6600946.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(1662..2339)
                     /locus_tag="ACHKUI_004934"
     CDS             complement(1662..2339)
                     /locus_tag="ACHKUI_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600947.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(2487..3167)
                     /locus_tag="ACHKUI_004935"
     CDS             complement(2487..3167)
                     /locus_tag="ACHKUI_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6600948.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(3370..4254)
                     /locus_tag="ACHKUI_004936"
     CDS             complement(3370..4254)
                     /locus_tag="ACHKUI_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6600949.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPELDLIIWILRSDERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     MFPAEEWNATEKCPSRHQELSLATVIARVATLFPSSFPVLPVAAPAGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDDAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            4355..>5075
                     /locus_tag="ACHKUI_004937"
     CDS             4355..>5075
                     /locus_tag="ACHKUI_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600950.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISKYDKSLDREIFYGVRDAAKNKTGKRIIEDAKILHQCRASKQVRKSFHKG
                     VLRSREWLNTQELLSRKHISVTGELFKFYIKDIIRLYCYKFLYSREYLNENAYKKRYQ
                     SDIHPRKVNVPILQLEVAWINNNCRSSDSSTDIIILPRMIRRMVVFNYTKINKQLEKL
                     LGGIARKQTSERNKGGLYTRIK"
BASE COUNT         1350 a         1166 c         1320 g         1239 t
ORIGIN      
        1 gtaataatgg accgtcatgg cataacactc cgtacgacag gcatgatgac tgtacgtcat
       61 cagacaggcg gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatgac
      121 gttaaacagc acccatgttt cgtcatcatc gtcatccggt tcgggtgcca taaatgcgcc
      181 accgttgttc agggtgtaca gattccagat accaccgcag tagtcttcac acagacggtc
      241 catccagccg aagacacggg gctccagggt cacccactgt ggaatgaggc caaaatgctg
      301 cggccagaag ctgatgcgct gttcatcagg gactatggtg gcaaccagct gaggctggtc
      361 attccctgat gcagcggtta cggaaacaga aggagtggtg gaattatgcg agacggttgt
      421 catgagatta ttccttataa aaagtaaatg aatggaagaa accccggggg aagggacaga
      481 cgtgagtcag aactgcgctt tcagggaaaa ggcatcagca catgctctcc agcagcgttt
      541 cagccatcac ccacaatgcg cgattgagct taatgtcggt gtcgatgctg tgaatggcac
      601 gggtatggat acgtttccct ctggcactgc gaccggaaat tccgcctttc agcatattct
      661 cctgaatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggtg
      721 tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagcgccg
      781 cctgtgccag cgcctggcgt gccggtggcg gcagaatcag cgactgcatg gcatcacgct
      841 tttcctcaat ccggtcaaaa acccccacca cctcgtaagc cccttcaatg actctgtcca
      901 ccacatttcc ccggtgcgga acacgcactt ctcccagcga ctgaccgcag accaggccat
      961 tggtgcagat ggcacgaaaa tatccgggta acatctggta gctggaggta ccgtcatgag
     1021 agttgagcag aataatttca gggacatgtt gtccgtttat ctccccgtca cggcgcagac
     1081 gcagcatgtg tttggtgtat ccccggcggc ccgggtcgcg cacgcgggtc tggcaggcga
     1141 agaatggctg aaaaccttcc cgctgcaggc tttccagtac ggtgatggtg ggaatgtacg
     1201 catagcgttc actgcgggag gtatgccgat cttccccaaa aatactgggt acatagtgca
     1261 tcagttcttc atgtgtcagc ggacggtcac ggcgtatctg gtttacatga ccaaaacgac
     1321 tggctaatcg cattatttgc tccttatgaa tgagttagcg gtataaacga aaaggccgcg
     1381 tctcccggag aagacgcggc ctgatggatg aaatgaagac gtttattgcc tgagaattcc
     1441 ctcactggcg agatacgaat ttagctgttt tccggcatca ggttcaaaat tccagcaccg
     1501 ccagcgtagc tgcccttcgg cattgcggat aaccagccgg aaatgcgttc cctggtcatc
     1561 ttcaataagc acattgcggt aagctgttgt gacagccatc gcctgttcgc gtgagaaagg
     1621 gccttcgggc aataaaacag ggtggcagtc cgacataccg gctattttcc gataaattca
     1681 acgctggcgc cggtggcatt accgtcagca ccaaccgtga cggtcacttt gtcgtacgca
     1741 cggtcttcag gattgaatac ttcaaactca accttattgc cttttacttc gtaagagcca
     1801 aaatcagcgg tttctgttgt ttcctcgtca tattcccggg cgtatgcctg ccgggcagcc
     1861 tgcaggacgg aatctttatt aatagtggtc gtctgtttcg gggaaaaacc ggcaagtgat
     1921 gtgacaaatt tcggacagac cttcagcata tcaccgcggg tgaattccat tcgtacacca
     1981 aatgcctgta aaacggacac atcacgctct gctatggagt gaacggcttt tttgaccatt
     2041 tccggcgcct gtccgatttc cacctcaaca gccctgaatc ccgtctgctg attacacatt
     2101 tgtgatgtgg ggatcaggtt accgttaata ctgatacggc tgcccaggag cgtctgtaca
     2161 ccattcgggc atccctgcat caggccggta ataccaatcg tgccattttt cagcattacc
     2221 agcgcctgac catcttccgt catggtctga caccagccgt ttttgacagc ccagttgccg
     2281 gcggaggcgg agaatgatgc cacagtgaac agcaggggaa aaagagctct ggtattcatg
     2341 actgacctct gaaatgaacg tacggtgtta ttgataatga tggaaggtat gcagtgactg
     2401 ttcagggaat gacgtagccg tatatttagt tttctgttca acatattgtc tccatacctg
     2461 tcgggacggc tttggcaggc cggcgatcac aggttaaagg tgttgataag tttatggatg
     2521 aaagagaaat gtggcagggc ggagataaaa cgctcttcag cgaccagact gtggatgtgg
     2581 cctctccgtt caaaccgttc tgatgtcagg ctgactgatt tgatatctgc cagaggaaag
     2641 acctgcagtt ttcccttctg taacgcgatc agataccagc taccgctgta ataaatgagc
     2701 cggcagggct ccagtgacgg ataccatcgc tcagtcatca gagagatgtg gatacactgt
     2761 cttattgcca ggatgagttg cgagaaacac tcggggaacg ttgcagtgat atccggcgcc
     2821 ggtagccaga taaggcaggg agagggctcc tggttgtcgg taagacaggt tatcagtcgt
     2881 ccgttccgga gcggaagtat ccgtgcgatc ccggtattct gtataaaaga aagcatttca
     2941 gggatcgcac ctgggctgct ctgtcgtctg aggctgtacc tgccatttct gtactctaaa
     3001 tccaggtgaa ccagacgctg atgaaaatcg cgctgtaaag tacgttctgt aacgccaaat
     3061 tcatctgaca gtgtttttag tgacagagat tctccggcca tcagtcggct gataatgagt
     3121 gataagcgaa ccgccaggcg atcatgccgg gaacgattat cagacataga tgtgcactcc
     3181 gtaagaaaac atcttttatt tcatgatgtt attatgcatc atgatgttga aaggatgtgt
     3241 cgcatccgga taatcaggaa taaaatgcag cagaaaaaat attcttttaa ttatctgatt
     3301 tttaagcatg atttcataaa aagaagaatg ctcgttaatc cagcgacaca tcctgtcggc
     3361 gtgagtgtgt cagaacagac gctcccacag tgtgatcagc aggtggataa tgcggtcccg
     3421 ggctttacgc agaagctgta acatccaggc cggaaaactg aaccgggcaa cggcgtcgtc
     3481 aaaacttttc ccgatggcat caccaaaagt ctgttgtgcg tgtttccggg cctgttcaga
     3541 gcggttttcc cccctgatat gtgaataaac tgcgctggtt gcctgtggtg gcagcgcgtg
     3601 gatcatcagt gacaccagcg ctggaaggtt ccagcctgca ggtgcggcta cagggagtac
     3661 cggaaatgat gaagggaaca gggtggccac ccgggctatt actgtcgcca gtgagagttc
     3721 ctggtgacgg gacgggcatt tttctgtggc attccattct tcagcaggaa acatgcgatc
     3781 ggcatggctg agaacaaaca gaaagcgcga gggatctgcg ccctcattca gtaaaaactg
     3841 atgcatggca atatcggcag catacgcacg ttcatcactc cgcaggatcc agataatcag
     3901 atccagttcc ggcagtaact gacgataaag cgctcggtat tcctgatcat gctgcggtgt
     3961 ttcaccaatg ccgggcagat cgaccagcgt cattctgcgt tcaccgagct gcagggtaag
     4021 acgatgagcc tggcgggtgc agccgttcag gggatgcgtg gcgcagatac gggactgaaa
     4081 aatggcatta cacaggctgc tcttgccggt gccggattta cccataatgc caatcacggg
     4141 ctcgtaatga ataacaccgt ggagatgttc aaggattttc tggcgtattt cttcaggaag
     4201 aaacgtcaga tatgatttca cttgcgcgga gagggaagaa ttgctgaatg gcataccggt
     4261 atcctgttga tttcactgaa ccggatcaca ctaatccggt ttatcattca ggtaatatgg
     4321 gtgtgaaaat ttttccattg acgggaagaa gagtatggct aaaaacaaag gtatttgtcg
     4381 gaataaaaaa tgcagtaatc ctgtttcaga atttgatgag gcaaatgcct taaggttatg
     4441 cgatgatcat tataagctat ggaagcgaaa taaagagaat cgtcgggtca ggaatatttg
     4501 tcgtataatc agtaaatatg acaaaagtct ggaccgggag atattttatg gagtaagaga
     4561 tgctgcaaaa aataaaacgg gaaagagaat tattgaggac gcaaaaatat tacatcaatg
     4621 ccgtgcctca aagcaagtaa gaaaaagttt tcacaagggt gtgcttcggt cacgggaatg
     4681 gcttaataca caggagttac tgagcagaaa acatatatct gttacaggtg aactatttaa
     4741 attttacata aaagatatta tcagattata ttgctataag tttctctaca gcagagaata
     4801 tcttaatgaa aacgcataca aaaaacgata tcagagtgat attcacccca gaaaagttaa
     4861 tgttccgata ctgcaacttg aagttgcatg gataaataat aattgccgtt caagtgattc
     4921 aagcactgat attattatcc ttccgaggat gataagaaga atggttgtat ttaattatac
     4981 gaagataaat aaacaattag agaaattgtt aggggggata gcacgaaaac aaacctcaga
     5041 aaggaataaa ggcggtctct ataccagaat aaaaa
//
LOCUS       DAZFRE010000193         5058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFRE010000193 DAZFRE010000000
VERSION     DAZFRE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.197"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(185..339)
                     /locus_tag="ACHKUI_004938"
                     /pseudo
     CDS             complement(185..339)
                     /locus_tag="ACHKUI_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(539..964)
                     /locus_tag="ACHKUI_004939"
     CDS             complement(539..964)
                     /locus_tag="ACHKUI_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6600951.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1043..1906)
                     /locus_tag="ACHKUI_004940"
     CDS             complement(1043..1906)
                     /locus_tag="ACHKUI_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6600952.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINIDPFQIAFATIGMD
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1906..2874)
                     /locus_tag="ACHKUI_004941"
     CDS             complement(1906..2874)
                     /locus_tag="ACHKUI_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO6600953.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2876..4534)
                     /locus_tag="ACHKUI_004942"
     CDS             complement(2876..4534)
                     /locus_tag="ACHKUI_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO6600954.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRAHPNNDPSRLIQISS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSGFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4568..4936)
                     /locus_tag="ACHKUI_004943"
     CDS             complement(4568..4936)
                     /locus_tag="ACHKUI_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600955.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1200 a         1402 c         1107 g         1349 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcat gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattaccccc ccccgccagg
      181 tctcttactt ctcagattcg tagtctacga agacagcgac ctccgtctgg ccggttcgga
      241 tccgtacctc gcgaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
      301 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      361 cggcacgtaa gaggctaacc tgcatgtgtt cagcatgaaa ttggcctcag attaatgtta
      421 agcgtcttgc aggacacgta atgttaactg gggcttttct ccgtctgcct tacggcagca
      481 tgcccgaggc agaccccctc aagcacccgc agtcattcta cagtaaaacc ccgcgctttc
      541 aatttcccgt atgtccggaa aactgactga cctgtgcccc tgatcgttca cgctcaacgt
      601 atctgtccat ttccagtttt attccctgca taatcaacat cccctcaata acaccttcag
      661 ctttttgcag tacccgcccg gcccagcaat cagagcgctc atgcttacgg cccagtccca
      721 taagcgtcat cccgaacaca taataatcat acagaaaatc atgcaaatcg ctgttgttga
      781 cgttcagttg cgccatgcaa ttgctgataa tcagcgcatc atcatcggta cactgaggcc
      841 gtgatttaac ctttgatggg atcacgccct taaatccggc agcgacgccg gaccatgcca
      901 cactctcatg attatttacc acccatgcac cccatcggga cagaacctgt tggatatcac
      961 gcattattct gtactccgct gtaaccatac tttttctgat ttgaatgctc agggggaaaa
     1021 tgtctactgg atatctccgg cttcattgct cacactcccc aaccagattg agaatgaccg
     1081 ctgaaccatt gtcttccatg tactcatctt ttccgcttgc cagaaaccag cgacacactt
     1141 caaccgcttc atcacgcgtc acaggcttta tttttcccag caaattttcg agccagaact
     1201 gccgatcata tactgactca tgatgttcac ctgtgaaacc gtatttgtcc cctgtttctc
     1261 tccccgctgt ctggcgcatc tcataaagcc agtcccagta aataaactca cgaacaacgt
     1321 ctgacaatgt atgaggctct ggcagtacat cacgatagcc atcaacatat gcacgacgct
     1381 gatcatcaat ttcatttgag cgaacaccgt caatacttcc ggcttttttc tctgccgcag
     1441 tccagcccca gtagtaatcg tcgataaatt tcggggaggt gcgaattatc cgctcagcct
     1501 ccacatcgtc gagtgctgcc tcatagctgc caaatgtggc cctgactgat gcggcttttt
     1561 tgatattctc acgggcattc ctgatcgcct gcgcggggtt atccatgcca atggttgcga
     1621 aagcaatctg gaaaggatca atattaatct cctgcaaata acgtccatac cgttcactcg
     1681 cttcttccgg cgttattttg agtttctcca gtgctgcttc ggctgcgtcc agatgtacgg
     1741 cctcgttcag gtgaataacc tccagaatcc agagataagc atcagtctgt ttatgtccgg
     1801 tgatcctcct ttgttctggc agaggtttga tatttgccgg aacggcgctg tacgctgccg
     1861 tcgggatagt gaataaagct ttatgtacct gattgtccgt acgcatcacg caaccgcctt
     1921 ttttctgtaa aaaaccagtt cccgaacctg atcgccgttc atgagcatat tgttgaaatc
     1981 atcatgatca ggccaccgga cacttacccg ctgaatgtca tttcgggcct ggagatttgc
     2041 atgtgcgcat tcacaggccg cagccattcc ggtagcactg tattcatcgc gatcggcaaa
     2101 aataatcaga tgggtgactc ccgccggtac gcggaacttt ttcataaaac cactgttaat
     2161 ggttgcccag gtatttactt tgtatatctg atgtgcggac aacgcggttt cgataccttc
     2221 agctataccc agtgtactgg ctaccggaaa catcctgata gcaactgacc gggcatggtc
     2281 cagatagtta tcgtcctgta aagatttgag ccttctggcg ctgataccaa tgtctgcttt
     2341 tttagctcca tcaagcaatg tctgatgcag gtaacacagt tctcccttgt catcggtcgc
     2401 cagggcataa agagactgga acatgcgccc gttataattt tgctggctgt tgaagcgaac
     2461 tccctcaacc ggaagactga atatgccacg ggaacgaaga tattcagcac cagatgttcc
     2521 ctgtagcgac gtgagcgctg aaaatcgacg ctgaacgcgc gatctcaggc tttccgacgt
     2581 acagatcaca ggcggtttat ctctccggta ttcattcccc gtgatgcggt ctatttcagc
     2641 gcaaatttca tgaaacgctc ttttttgtgt cagagcgatc agtttcatgc cgtcaccgct
     2701 accacaaaca cagatccacg ttccggtgcc atcacggtca tcaatacgga acttacctct
     2761 ggcaccacaa accggacatt cccctttgaa gtgcttttta cccgtgactg gcggcagtcc
     2821 gtaatgtttt aaaatttcag gccagtggcc tttcgctgct tctgctgttt tcatcttact
     2881 gactcagact gtttctgttt ttctgaagtt gttgctttgc ctgcatgatc tgccacgact
     2941 cactgccttc cggcacccca gaccggacac catgaagcag ggacagagaa agcgcttcgg
     3001 gggatttttc cgtcatcttc tctgcccggc tacgccctct ggcaaaccgg atgaatttgt
     3061 gtctgatgta attactgacc tccggggtga tttccatcgg aaaaccgctc agcccgtcag
     3121 gccattcgcc gaacttctcc cggaaagtgt gagcgcacca tccatcgctg accggtcgcc
     3181 ccagagaggc acgctgacgc tgatagaatt tgatctgact ccaccaggcc tgtttgtcgc
     3241 ttttggtgta aattttttcg tccttgctga gtttcctgat attgcggcgg gtatctgtgt
     3301 tcacatcctc accagccagc ggtttaaatc cgcatttcgg acagacataa acacctgccg
     3361 gtttcatgaa atgacacccc ggacattctt tcggcagttt ttcttcccga atttcgttac
     3421 cacgtgatga tgacacctgc atcccgtcgt ttttgccggg cagttcgtca tactcaatat
     3481 tttcaggaaa tcccaggcga tgaaccgtac cggaatggtc gaaaatcagg catgactcct
     3541 tgcctggcgc tgtacgcaat cctctcccaa tcgcctgaag ccagcgaatc tctgattttg
     3601 tcgggcgtgc gtaaatgatg caccggacat cactgtcaaa tcccgccacc agaacgccaa
     3661 cgctgacaat gattttcgtc gctcccgttt caaaacgact gatcattaac tgacgctcat
     3721 catgtggcgt ttcagcaacc atcacctctg cgttaattcc ggctttgttg aactgcatgg
     3781 tcacgaagct ggcatgcgtt ttattgacgc agaacgccac tgtcggtaaa tcccttccgt
     3841 tacacagcca gttatcgaca atatcgccaa ccagatccgc gccgcacata atctctgcca
     3901 gttgtgattc gtcgtaatcc ctgccgtact ccattgagac tttcgttttt acaccttcca
     3961 gatccggttt cgtgggcgca taaaattcgt aattgctcag ttcgccacgc tggatcagtt
     4021 cccctatagt ggtcggcttg agaagacgct ggtaataacg ccccaggaac gatgaaaagg
     4081 gtgtaccgga caacccaatc accttcacat ttccctctgc aaccagtcgt tcgatttccg
     4141 tgaggatgcg gcgcttacgg agatgcgctt catcaataat cagcaggtcg atattctgag
     4201 gaaaatcacg gcgaatcagc gtatccgcac tggaaatctg aatcagtcgt gacgggtcgt
     4261 tattgggatg agcacgccag ataaagctga tttcatcccc ggaaagcccg tactgaacaa
     4321 agcgctgtgc tgtctggttg atcaaaatgg tataaggaac gacaaacaga acgcgcatgc
     4381 cacggcttac cagaccagca gtgatgaacg ccgccaggcc ggttttacca ctccctgtag
     4441 gcatgtacat catgaaggac tgatatgttt tccagtcatg acgcaacata ttcagcgcac
     4501 gttcctgagc aaaattcggc ttgatattca gcatggtatt ctcccgatga caaggctgat
     4561 acagtgatta cttaaaccgg gtcgcccgga tgacatccac accatcggcg atataacgct
     4621 gtggatgttt gccatcacgg agaaaaacaa atcccgaaat gccttcagga agcgctcgaa
     4681 ttttcatcag tgaacgctga cggggtgatc ggttatccac ctcaactgcg caacgctgac
     4741 catcgggaga aataaccaga agatcgataa atcccctgcg atcacctttc agcctgacag
     4801 tgaaacagcg ttgcaactca cagcccagag atttgatttt cttttgcaga acgtagtcga
     4861 atgtgacctt gtccattcca tgtccgaatt cctcttccag aaggttgatt agtaccagtt
     4921 tcagttcagc gctcatattt taaccatgat gtactagttt gctgatacgt ccgttttttc
     4981 agggatgccc ctttagatcg agatctacct aacctctgta cccgtctgtt ggaagatccc
     5041 gttccagtgc ttcgcact
//
LOCUS       DAZFRE010000194         4961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFRE010000194 DAZFRE010000000
VERSION     DAZFRE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.198"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            66..818
                     /locus_tag="ACHKUI_004944"
     CDS             66..818
                     /locus_tag="ACHKUI_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6600956.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVCDVIRKNLDLDIHIQTVFCIKRVRYMGSIPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            832..1908
                     /locus_tag="ACHKUI_004945"
     CDS             832..1908
                     /locus_tag="ACHKUI_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6600957.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            1908..2906
                     /locus_tag="ACHKUI_004946"
     CDS             1908..2906
                     /locus_tag="ACHKUI_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6600958.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAATWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2911..3825
                     /locus_tag="ACHKUI_004947"
     CDS             2911..3825
                     /locus_tag="ACHKUI_004947"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6600959.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGRDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            3853..>4961
                     /locus_tag="ACHKUI_004948"
     CDS             3853..>4961
                     /locus_tag="ACHKUI_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6600960.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHTSLIFVSDNNFQPHQQTKFYLFIDKE"
BASE COUNT         1455 a         1051 c         1124 g         1331 t
ORIGIN      
        1 caccatgcgt aataaaagtt aaagaatgta gagttggtat caaccacttg aaatgaggct
       61 ttgtcatgat aaaaatttat aaagccagta aaggtcagct aaagcatcgt gaaatctttg
      121 atgaaataaa gaaaaaagta gataacggtg aatggaaaga aggcaaagcc ataccgacag
      181 aaagggaatt gtcagaatat tatttaagta gcagaacaac tattcgaaag gcaatagaaa
      241 gtcttaaaca acgcggctac ctgcatagtg tccacgggca aggaacattt gtattgcctt
      301 ctcgtagtag agaaaatcat ttattgcata gttttaccga cgatattaaa gctcgaggtg
      361 gtataccagc acagaatatt cttgagatag gctatgttcc tgtctgtgat gttatcagaa
      421 aaaatctcga cctggatatc catatccaaa ccgtattctg tataaaacgc gttcgctata
      481 tgggaagtat ccccctgggt attcagacat catggcttgc attaaatgac aggcaagaaa
      541 taacccaaga agatctgctg gcgacgggtt cgctctatat tttactggaa gaaaaactcg
      601 ctattaaacc tttagaagca acagaaatta tctctgcccg gcttccctcg ccccttgagc
      661 gtcaattact ggaactgact gccgatgatg tcgtattaag ttgtacccgc gtcacgttat
      721 ccgtggaacg caaaccaatg gagtatgtag agatggtgta tcccgcaagc cggtactcct
      781 acaaagcacg aattacaaaa gacagtttta gcgtgtaaca gagagaatat tgtgaacaaa
      841 atagaacaag ctgtaattta taacaaaata ttaggttctt tagcctgcgc aggtatgggc
      901 gatgcattag gcgcagcgac tgaactctac agcattgatg aaataaaagc tcaatgggga
      961 ggttttttaa atgcctttgt ttctcctcct gccgatacat tcgctggctc attaaatggt
     1021 attgcgggac ttatcaccga cgattccagc caaatgtatg tgttttcaga agggttaatt
     1081 gaggctggat ttgataactt caccaataat gactggttag cttgtcttct gcgttgggcc
     1141 gatatgcagc cctacgcaaa ttataaaggc cccacaacag aacaaatcgt caaggcacta
     1201 aaagaaggta gaccaacaaa caccattggg cgtattggaa catcttctcg tcaggcaccc
     1261 aatgtcggca ccacgaatgg cgcaggaatg cgtgtagccc ctgcaggatt gatctggcca
     1321 ggcaagaaag aaaaagcgtg tcacctggcg ctaataactt gcctaccttc gcatgacacc
     1381 aatattgcga ttgccagcgc ttgcgccatc gctgcggcga catcacaagc tatgttacca
     1441 gaagcttcgc tcacatcatt actggacgcc gcaatctggg gagcgaatta cggcgaacgc
     1501 ctggccaaac aatatgcacg atgtgtcgca gggccatcta tcgccatgcg catccaactt
     1561 gcagcggaca tcgccagacg agccaatgac ctggagtctt gtctgcgaga aatggaaggt
     1621 ttggttggta actctgttgc cgcccatgaa tccattcctg cggcaatcgg tctattgctg
     1681 tactgcaaag gagaaccctg ggaaaccatt catgcctgcg ccaatattgg caatgacacc
     1741 gatagcatcg ctacgatggc aggcgcaatt gcaggggcat ggcgcggttt tgatgcgctc
     1801 cctgaagata aatatgcctt tttccgcgcc gtaaataata aagatttcga tattgaagcg
     1861 atcgcatcgg gtctgacatt gcttgcatta gaggcacagg agaaataatg aacatgccag
     1921 cccgtactcc tgttatgcca ttagcgcaaa ctcgtgagga aataaatgct acggcggcat
     1981 cgttacgcca tttggcagct atctggggtg aatgtttttc tacttttcag ctcgataaac
     2041 gtgctgttga acgcgtctgt atagtgggat cgggcgactc ctggacggtg gcactatgtg
     2101 ccgccacctg gctggggaaa tacacacatc tgttttgtta cgcattgcaa acgtgggatt
     2161 ttcttcagac tgatttaacc cgttatcaga aagaaacgtt aatcattatt ctcagtgcaa
     2221 gtggcagacc atcaatgacc gttgacgccc tctgccacgc agtatgctca aacgcgcaag
     2281 tgctgggtgt gacgaattgc cctggaacac cattttgcgc aataacagaa aacatgctct
     2341 atacatgggc gaataagcag ggtattccaa cgcaaagtag tagcgtcaca ctttatgccc
     2401 tcctgcgctt agcgcaaaaa ttatgtccag gcttaacacc actgcagatt gaagatgatt
     2461 tggaagggaa atttagccag attaatcagc actggcagca aaagaagcgc catttttacc
     2521 aacaaaaaga gataacgttt cttgggagtg gcttaagttg gggactggca atatcgggga
     2581 gcaatttact ttcttgcggc ccacagattc gtgcgacagc ccttccactg gaagaatttt
     2641 atcattcatt acgcttacat caggctggtg tcggtcagca ttatatttta ctaccagcca
     2701 catcctgcga gtccccattt taccttgcaa cccaaaaagc aattgttgaa cagggagcga
     2761 cagcagaatt aatcagtttt atacctgacg ccagtgagtc caacaattta tttctggtca
     2821 tgcagtggtt atatgaaatg tgctggcatt taagttgtga ttatgtcgac gcaggcgggc
     2881 aacgcgttag ccatagggag aaataagact atgaagaaat ttgatgtcgc cgctttaggc
     2941 agtggaaata tagatatttt tttatctatc ccgtcactcc ccactcgtgg cggtaaagtt
     3001 gtcgggacac gtctgggtga acaagctggc ggcacagtcg ccaacagtgc ctgtgcgatg
     3061 ggacaactgg gtctcaatgt ggtatctgtt tcatgtgtgg gcaatgatca ttcagcctcg
     3121 atcattcttg atggatttaa aaaatatcat gtaaattgtg atttcgttca ggtaattccg
     3181 gaattgattg ccaataccgc gattatcttt attgatgaat taggtgaaaa aacgttggtt
     3241 tactcgcctg gtagggatca tgagtgggat aaagaaaaag ccctgcaggc aattgcgcaa
     3301 agccgttatt tttatacgat gccagcagat atcgagaaat ttcgtatgct ggccgaatat
     3361 tcccgtagcc agatgacaaa agttgtcgtt gatattgagc ctcatatcat tgcaacacct
     3421 gaacaccttg ccagaatttt acaccttgct gatattgcga tatttaatta tgacggtttt
     3481 attcgtggct atgcaacaga gccagatttt acacttctgc atgatataca agacgagtat
     3541 caacttgacg ctgtcgtggt tacgctggat gttaggggag taattgcagt caaaggcaat
     3601 gagcaagtaa aaataggtag ttatacaatt cccgttattg ataccacagg cgctggagat
     3661 acttttaatg gtgcatttgt ttatagctta ataaaaaata tgcccttaat tgaagcacta
     3721 aaatttgctt ctgccacagc agcaataaat attactgcat taggtgccag aggtcatctg
     3781 ccgacaccat cggaggttaa tctttttctt tcacaacacg actaaaatca tatatataca
     3841 aaggtaaata tcatgaaaat aagatatgcc gctatttttc tggcattacc gctgttatgc
     3901 acaacagcgc aggcaaacag ttttcttcat tatgctgggg agtttaactt ccggacggga
     3961 gaaaagcaga acaatgtcac aatcgcgggg ctatcagcca tcacatttga tgcaaaaaaa
     4021 aatattttct atgctattaa cgatagcaga aacaataata aagaaggaga tgccagtctt
     4081 tatacactaa aaatacaggt atcatccagg ggcattgagc aagtgaattt tctcaaccag
     4141 cgtccactgc ttgatgcaaa acagcaacct ttcgtgacca atacggtgga tgccgaaggt
     4201 ctggccctga cacacaatgg taaatcatta ctctggagtt cggagttagg tgctccactg
     4261 cgtctgagca ctctcgatgg cgtaatggag aaagatttta cgtctttatt tcctgctcgc
     4321 tttaatatta gttctgacaa agagagcagc aatggtattc gcagcggcaa cgcctgggag
     4381 ggcctgacag ttacaccaga tggaaaatca ctttttatcg ccgttgaaag cagccttaaa
     4441 caggatgggc ctattgcaag tccaataaat tcaggcacat cacgcttact gcaattttct
     4501 atagatgcag acggtcgacc ttcaaagcaa ttgcatgaat atctctatat tactgatcct
     4561 gttccgcaag tgagtaagtt cggtattaat gataatggcg tatccgaggt catggcgctg
     4621 aatgatcacc aattgttggt catcgaacgt tccggcagaa acgtcagtgc tgggttcaat
     4681 gactgggatt actctgtcag agtttatatg gtcgatttaa ctgctgccag cgatatcaag
     4741 aatattgaca gtctgcaaga ttggtccaat aaatccacgt tacaacccgt cagtaaaaaa
     4801 ttattaattg attttgctga ttacacttcc tctgcggatt gtattgaagg ggtaaccttc
     4861 ggtcctctga ttgatgggca tacatcgtta atatttgtat ctgataataa tttccagccc
     4921 catcaacaga cgaaatttta tctattcatt gataaggaaa a
//
LOCUS       DAZFRE010000195         4814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFRE010000195 DAZFRE010000000
VERSION     DAZFRE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.199"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            122..1096
                     /gene="hemB"
                     /locus_tag="ACHKUI_004949"
     CDS             122..1096
                     /gene="hemB"
                     /locus_tag="ACHKUI_004949"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6600961.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1136..1987)
                     /gene="tauD"
                     /locus_tag="ACHKUI_004950"
     CDS             complement(1136..1987)
                     /gene="tauD"
                     /locus_tag="ACHKUI_004950"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6600962.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAHRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1984..2811)
                     /gene="tauC"
                     /locus_tag="ACHKUI_004951"
     CDS             complement(1984..2811)
                     /gene="tauC"
                     /locus_tag="ACHKUI_004951"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6600963.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWVVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2808..3575)
                     /gene="tauB"
                     /locus_tag="ACHKUI_004952"
     CDS             complement(2808..3575)
                     /gene="tauB"
                     /locus_tag="ACHKUI_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6600964.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3588..4550)
                     /gene="tauA"
                     /locus_tag="ACHKUI_004953"
     CDS             complement(3588..4550)
                     /gene="tauA"
                     /locus_tag="ACHKUI_004953"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6600965.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDIPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1120 a         1383 c         1223 g         1088 t
ORIGIN      
        1 caaagatgtc ctgacctctt tttcattctc tttttgtcaa catcgcgaca actttcgtaa
       61 aacacaccca gcctgcttca ggtatactat gctcctcgat tccacaaaca tcaggcagac
      121 catgacagat ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat
      181 gtttgaagag acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga
      241 aattgacgac tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca
      301 tctggcacgc gaaattgaac gcatcgccaa cgccggtatt cgttccgtga tgactttcgg
      361 catctctcac cataccgatg aaaccggcag cgatgcctgg cgggaagatg gactggtggc
      421 gcgtatgtcg cgcatctgca agcagaccgt gccagaaatg atcgttatgt cagacacctg
      481 cttctgtgaa tacacatctc acggtcactg cggtgttctg tgcgagcatg gcgtcgacaa
      541 cgacgcgact ctggaaaatt taggcaagca agctgtggtt gcagctgctg caggcgcaga
      601 cttcatcgcc ccttctgccg cgatggatgg tcaggtacag gcgattcgcc aggcgctgga
      661 cgctgcgggc tttaaagata cagcgattat gtcgtattcg accaagttcg cctcctcctt
      721 ttatggcccg ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca
      781 gatgaaccca atgaaccgtc gtgaggcgat tcgtgagtca ctgctggatg aagcccaggg
      841 cgcagactgc ctgatggtta aacctgccgg agcatacctc gacatcgtgc gtgagctgcg
      901 tgaacgtact gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa
      961 gttcgccgcg ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc
     1021 gattaagcgt gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa
     1081 gaagattctg cgttaatgat cattatgccg ggttatccaa cccggcattt cccccttacc
     1141 ccgcccgata aaacggaata tccccaagaa tcgtcgcccg atgcataatc cgccgctgtg
     1201 gcaggtaatc cgcattggca tagtgctgag tcacgcggtt atcccaaatc gcaatatcat
     1261 tcggttgcca gcgccagcgc acctgaaact ccggtttagt gatatgggca aacaaaaaac
     1321 ctaacaaggc ttcgctctct ttctcgctga catcaacaat tcgtgtggta aaaccttcgt
     1381 tcacaaacag cgcctgttta ccggtcaccg gatgcgttcg caccaccgga tgtagcaacg
     1441 gtggattttt cgcgaccgcc tcacgccagc gttgatgctc agcctcggtt ttgcggtact
     1501 tgtattccgg gaacgattta cggaaatcat gctccgcacg caacccgctc aacagctggc
     1561 ggaagggaac agagagcgcc tcatatgcgg caataccact ggcccagagc gtatcgccgc
     1621 cggtcgaggg taactctttc gcagccagaa tcgcccctgc gggtggcgtt tgaataaatg
     1681 tcacatcggt gtgccagttg tcgttatccg gcggattatc gttatgggta tccaggacga
     1741 tgatctcgtc aaccccttca gcatgagggt aaacagggtg aatatgcagc tcgccaaaac
     1801 gatgggccag cgcgcgttgc tgctgcggcg taatagcttg atcgcgcaga aacaccacct
     1861 gatggcgcag caccgcatgg taaagctgtt caaactgatt atcgcttaac gggcgcgtta
     1921 agtcggcccc cgtaatttgt gcgccaatat acggccccag cggggtaatg ctcagacgtt
     1981 cactcattgt acttctccat gccagggcgt caggcggcgc tgtaacgcgc gcagacccag
     2041 ttctaaaaga aaggcgataa tcgcaatcac cgcgatcccc gccagcacca cgtcagtcgc
     2101 gagaaattca cccgctgact gaaccataaa tcctaaaccg cgcgttgcgg caatcagctc
     2161 cgccgccacc agcgtagacc agcccacacc cagaccaatg cgtaatccgg tgaggatttc
     2221 cggcagcgcg ccgggcaaaa tgacaaacca cagcacctgc gcacggctgg cacccagcga
     2281 ctgggcggca cgaatgcgaa cctgctgcgc gcttttcacc cccgccagcg ccgacatcgc
     2341 caccggcgca aaaatcgcta gatagatcag taagatcttc gatgtttcgc caataccaaa
     2401 ccaaatcacc atcagcggca aataagccag cggcggcacc ggacgataaa gctcgattat
     2461 cggatccaga atgccgcgca ccgtcgggct tagccccatg gcgatcccga ccggaatacc
     2521 gatcaacacc gccgcgagta gcgccagcaa aatgcgcgtc aggctggctg ccagatgctg
     2581 ccacagcgtg gcgtccataa atccctgcgg cccggcaatg gtgagcagtt ttgccagtac
     2641 ctgttgcggt ggcggcaaaa acagcgggct aatcagttgc agcgccgcga ctacccacca
     2701 tacagtgagt aaaatcgcta acgtgccaat gcttaaggtc acctgacgcg agagcggcca
     2761 gcgccatttc agccgatgcg aatgcagttt ttcattaatg agcacactca tgagaacgcc
     2821 tcccgttgct caaatacgcg gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg
     2881 gacttgatgc tgcggctcga ctctcccgca acaaagcggc gggcgaagtt gagcggcagc
     2941 cgctccagca cgcggcctgg gccgggtgaa agcagaacca gttcagtcgc cataaacacc
     3001 gcttcttcta tatcgtgggt aatcaacagc acctgctttc ccgtctcctg ccaaagtttc
     3061 agcagcaggg tttgcatctg gtcgcgggtg aaggcgtcca gcgcaccaaa cggttcgtcg
     3121 agtaataaca gctggggatt tgccgccaac gcgcgggcaa tccccacccg ctgacgttga
     3181 ccaccggaaa gctgccagat gtagcgtttt tctgcgcctt ccagcccgac ttttttcacc
     3241 atctggtgtg cgatttccag tcgctgcatt ttctctacac ctgccagttg caggccgaac
     3301 gccacgttgt cctgtacatt gcgccacggt aacagccctt cattctgaaa aactacgcca
     3361 cgctctgctc ccggtccctg aataccctta cccgccagtt gaatgctgcc atgctgataa
     3421 ggcacaaaac cagcaatcaa attcagcaac gtagttttgc cgcagccaga cggccccaga
     3481 actaccagaa gctcgccgct ttccagcgtc aggttgatat cttccagtgc cggtttgccg
     3541 ccataatcgg cgtaaagatg agaaatttgc agcatctgcg cctcctttta ttgcacgaag
     3601 cgcgaggtaa cgtactggct gtaatcattc gctacagccg ggactttgcc ctgctctttc
     3661 aaaaactgcg cggtgtcgat gatcgctttg ttcaccggtc cggtcagttc tgccgtttgc
     3721 tgctgcggcg tcagataggt attccccttc accagccccg gaatgtcacc ttccggcacg
     3781 ccgcttaacc gcgccagttt gctgatgttt tccggctgtt tcagccacgc gtctgggtta
     3841 gcaatgtacg gttgctgagc atcgatggcg cttttagcga acgctttcac gacctcagga
     3901 tgtttctcgg caaaatcttt ccgcacgacc cagacgtcca gtgtcggcgc gccccactgc
     3961 ccgacctgtt cagaatcggt cagcaccttg ccatcttttt ccagggcgtt aaccgccggt
     4021 gcccagacat aagcaccatc aatatctccc cgctgccatg cggcgataat cgcgggcggc
     4081 tgcaggttca caatctcaac ttgcccaggt ttaatacccc agtgtttcag cgccgccagc
     4141 aggctgtagt gggtggtgga gataaacggt acggcgatgc gtttaccaat caagtcttcc
     4201 ggtttactga tagttttctt taccaccagc gcttcggagt tacccagttt tgacgccagc
     4261 aagaagactt caatcggcac ctgttggctg gttgcaaccg ctaacgggct ggaaccgagg
     4321 ttgccgattt gcacgtcgcc cgaagccagc gcccgcacga tgctggctcc gctgtcaaac
     4381 ttacgccagt ccacggttgc tccgctttct ttagcaaagg tgttgtcggc ctgagccact
     4441 ttcgccggtt cggcggaggt ttgatacgcc acggtgacgt tcaccgcctg cgcctgaaaa
     4501 gcgatgaatg ccagtgcggc aagaagtgtg ttacgcgatg aaattgccat gattgtctgc
     4561 tcccccgtct tgttatggga gcagtattca ggaataaaaa cattcattaa aagaattagt
     4621 cgttatcgta cagatgattt tattcttagc aaaaaagcgg caaaggaaag attaaatatg
     4681 caattacgcc cactctgata actcagtaaa ttactgcact tcattttatt aatgcataaa
     4741 caagcaggtt ctaatgtctg ttattttatt aacagcaaca catataatga aaccattata
     4801 ttcacattgc atat
//
LOCUS       DAZFRE010000196         4783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFRE010000196 DAZFRE010000000
VERSION     DAZFRE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.200"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(148..>4783)
                     /locus_tag="ACHKUI_004954"
     CDS             complement(148..>4783)
                     /locus_tag="ACHKUI_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HFO6600966.1"
                     /translation="NSGNTTVDGQGSTGTEIAGNNAVVNQDGELDVSGGGHGIDITGD
                     SATVDNKGGMTVADADSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNNGN
                     TTVDGKDSTGTEIAGNNATVTQEGELTVSSGGRGIDITGNNAKVDTKGKMTITGTDSV
                     GVSINGDSATLTNTGDIDVSNSATGFSLVTNEGIISLAGSMKVGDFSTGMALSGDNNS
                     VTLAAKDINVTGQKATGVNISGESNTVDITGNILVDKDQTADNAVDYFYDPSVGVNIS
                     GNSNTVSLDGKLTVIADSELTSRKYMEFDGSQENISGLTVSGDGNTVNLNGGIQFVGE
                     KNALADGSTIADKRSYFGKTPLVSVDGQSKVYLNGDSTISGSLPLGYANILQLSNKAA
                     LEIGSDATFSMQDISVYEHYFAQTPQIIKVDTGSQVVNNGDVDIWNISFAGIWGENST
                     GINNGNITLSQYDYSSPETSFSEPDHMAFLSSSGGSAVNNGTITAKVMEQHSVLNLGS
                     TAGVADPRVFNNSVSSMMGMEAYGKGTVLNSESGVIDMYGRGNIGMLAVDDSAADNAG
                     KITLDTLWVDQNDTTTLRTDLPSSTAIDYGVGMATGTNSGGGARSNGVATNQQGGVIT
                     VYNAGAAMAAYGASNMVINQGIINLEKNGNYDGSLGANMLVGMAVYNRGTAINDKTGV
                     ININVDTGQAFYNDGTGTILNYGEINLLGSPMDSADSHMGAIPENLDLLTALTGSGET
                     DMRTASSGGFVTTKALANYGNETLNSNVAAKAWLYNQDKANLTINGELSIGQGLENSG
                     LLNSDTISAAANVYNRASGSIITDQLSLTGSNSFFNEGNFSGSVAGSSYKQNVVNTGT
                     MAVMADGKSLISGSFLLYNEAGATLSNSSSAVSGGENAIVNVTRTGDSLAQVNRGTIT
                     AVNGYSAIKTASTGSNSNGKWIWNTDTGVISGVNPNAPLIDLGRGYNFANAGTINVQG
                     DGAVAISGGTTSYTVQLVNSGTINVGTAQGQADGTNGTGLIGIKGNGSDTTINNAQSG
                     VINVYADNSWAFGGKTKAIINNGEINLLCDTGCDIYAPGTTGTLNDHNSTTDIIVPAA
                     TSTPTQGSVPTVPADSSAQQKLTNYTIGTNSDGTSGMLKANNLVISDNVKVNTGFSAG
                     TADTTVVINDVFKGENISGAENISSSTVMWNAQGSTDASGNVDVTMTKNAYTDVVTDS
                     SVNNVAQVLDSGYTNNDLYTSLNVGTTAELNSALKQISGSQATTVFNEARVLSNRFSM
                     LSDAAPEVANGLAFNVVAKGDPRAELGNNTQYDMMALRKSMTLTEYQNLSLEYGIARL
                     EGNGSDTVGDNGVTGGYSQFFGLKHQMAFDNGMSWNNALRYDVHNLDSSRSIAYGDVN
                     KTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLEGGYQERNAGDFNLSM
                     NSGSETAVDSIVGLKLDYAGKEGWSANATLEGGPNLSYVKSQRTASISGAGSQRFNID
                     DGQSGGGFNSLATMGVKYSSQESALQLDAFHWKEDGISDKGVMLNFKKTF"
BASE COUNT         1078 a         1322 c         1065 g         1318 t
ORIGIN      
        1 ataatgcgaa ggcgtaaaca agattactgg atgagtagct ctaatatcct gaaagacacc
       61 aaaacaaaac gcgtcaagca ccgcatccgg caataaaaaa accgcaacct gtatcagcgt
      121 tgcggtttat gtattacatg ctaaaaatta gaatgttttc ttgaagttta gcatcacacc
      181 tttatcactg atgccgtctt ctttccagtg gaaggcatct aactgcaatg cactctcctg
      241 gctactgtat ttcacaccca tcgtcgccag gctgttgaaa ccaccgccac tctgaccatc
      301 gtcaatgttg aaacgttggc ttccagcgcc cgaaatactc gcagtacgtt gacttttcac
      361 atagctcaga ttcggcccac cttccagcgt ggcgttcgca ctccagcctt ctttccctgc
      421 atagtccagt ttcagcccaa cgatgctgtc caccgccgtt tcgctgccgc tgttcatgct
      481 caggttaaag tcacccgcat tgcgttcctg atagccacct tccagcgtat gacgcagttt
      541 cacacccgca taaggcgtca cattcagacc ttcacgcagt tcaaaggttt ttgccccttc
      601 actacggaat tccagatact gctgtttcac attagcatca gcggttttgt tgacgtcgcc
      661 gtaagctatc gagcgactgc tatcgagatt atgcacatca taacgcaggg cgttattcca
      721 gctcataccg ttgtcaaacg ccatctggtg tttcagaccg aagaactggc tgtaaccgcc
      781 agtcacacca ttatcgccta cggtatctga accattaccc tccagacgcg caataccgta
      841 ttccagactc agattctgat attcagtcag tgtcatggat ttacgcagcg ccatcatgtc
      901 gtactgtgta ttatttccca gttcagcacg cgggtcgcct ttcgctacca cgttgaaggc
      961 cagaccgttt gcaacttccg gtgctgcatc ggacagcatg ctgaagcggt tacttaacac
     1021 tcgtgcttca ttaaataccg ttgttgcctg gctgccgcta atttgtttca gcgcactgtt
     1081 cagttcagca gtcgttccta cgttcagact agtgtacagg tcgttattgg tgtaacccga
     1141 atccagtacc tgagcgacat tattaactga agaatccgtc acaacatccg tataggcgtt
     1201 tttggtcatc gtgacgtcta cgttgccgct ggcatcagtg ctgccctgag cattccacat
     1261 taccgtggaa gaagaaatat tctcagcccc ggaaatattt tcacctttga aaacatcatt
     1321 aatcacaaca gtcgtatcgg cagtacctgc actgaacccg gtatttacct tgacgttgtc
     1381 gctgatgaca aggttattgg ctttcaacat accagaggtg ccatcgctat tggtaccaat
     1441 agtgtagtta gtcagttttt gctgtgcact ggagtccgca ggtactgtgg gtactgaccc
     1501 ctgcgtaggt gtgctcgttg ctgcaggaac gataatatca gtagtactgt tatgatcgtt
     1561 cagcgtaccg gtggtacccg gtgcatagat atcacatccc gtatcacaca gcaggttaat
     1621 ttcaccattg ttaatgatcg ctttggtctt tccaccgaag gcccaggaat tatctgcgta
     1681 aacgttaatt acaccgcttt gtgcgttatt aattgtcgta tcactgccat tccccttgat
     1741 accaattaac cctgtaccgt tagtgccgtc cgcttgacct tgcgccgtac cgacattgat
     1801 ggtcccacta ttcaccagtt gaaccgtata gctggtggtt cccccgctga tagcgactgc
     1861 accatcaccc tgcacattaa tagtgccggc gttggcaaag ttatagccgc gaccaagatc
     1921 aattaacggt gcatttggat tcacgccgct aattacaccc gtgtcggtat tccagatcca
     1981 ttttccgttg ctgttagaac ccgtacttgc cgttttaatt gcactgtagc cgttgaccgc
     2041 agtaatggtg ccacggttaa cttgcgcgag tgaatcgccg gtgcgggtta cgttaacaat
     2101 ggcattttct ccacccgaaa cggcgctgct gctgttgctg agtgttgcgc ctgcttcgtt
     2161 ataaagcagg aagctgccac tgataagcga cttaccgtca gccattacag ccatcgttcc
     2221 cgtgttaacc acattttgct tatagcttga gccagcaacc gaaccgctga agttgccctc
     2281 attgaaaaac gagttgctac ccgttagcga taactgatca gtgataatgc tacccgatgc
     2341 tcggttatag acattcgcgg ctgcgctaat ggtgtcagaa ttgagtaagc cgctgttttc
     2401 cagcccttgc ccgatggata gctctccgtt aattgtcagg ttggccttat cctggttata
     2461 aagccacgct ttggcagcca cattgctatt cagcgtttca ttgccgtagt ttgccagcgc
     2521 tttggtcgtc acaaacccgc cacttgaagc agttcgcata tccgtttcgc cgctgcctgt
     2581 cagtgctgtc agcagatcca gattctccgg aatagccccc atgtgagagt ctgcactgtc
     2641 cattggcgag ccgagaaggt ttatctcacc atagttcaga attgtccctg taccgtcgtt
     2701 ataaaacgcc tgccccgtat cgacgttaat attgatcacg cctgttttat cgttgatagc
     2761 ggtgcctctg ttatagaccg ccattcccac gagcatattc gcgcccaagc tgccatcgta
     2821 atttccgttt ttctccagat tgataatgcc ctggttaata accatgttgc tggcaccgta
     2881 agcagccata gcagcaccgg cattataaac agtgattaca ccgccttgtt ggttagtcgc
     2941 aacaccgttg cttcttgcgc caccaccact attcgttccg gtggccatac caacaccgta
     3001 atctatagcc gtgctggaag ggaggtcagt acgcagcgtc gtggtgtcgt tttgatcgac
     3061 ccaaagcgta tccagcgtga ttttcccggc gttatccgct gcggagtcat caactgccaa
     3121 cataccaatg ttgccgcgcc cgtacatgtc aataacgccg ctttcgctgt taagaacagt
     3181 gcctttaccg taggcctcca ttcccatcat actcgacacg ctattattga ataccctcgg
     3241 gtctgcaaca ccggctgtag agcccagatt gagaacactg tgctgttcca tcacttttgc
     3301 agtgatcgtt ccgttgttca cagcactacc gccactggag cttaaaaagg ccatgtggtc
     3361 aggttctgag aacgatgtct ccggagaaga atagtcatat tgagaaagcg taatatttcc
     3421 gttgttaata ccggtagagt tttcgcccca aatgcctgca aaactgatat tccagatatc
     3481 cacatcacca ttgttgacga cctgtgaacc cgtatcaacc ttgataattt gcggagtttg
     3541 cgcgaaatag tgctcgtaaa cactaatgtc ctgcatactg aacgtagcgt cactaccaat
     3601 ttcaagcgcc gccttattgc ttagctgaag aatgttggca tagcctaacg gcaaagagcc
     3661 gctgatagta gaatcaccgt tcagataaac tttggattgt ccatcgacac taacaagagg
     3721 tgtcttacca aagtagctgc gcttatcggc aatagttgaa ccgtctgcca gcgcgttttt
     3781 ctcgcccaca aactgaatgc cgccattcaa attgacggtg ttaccatcgc cagacacggt
     3841 aagcccagaa atattctcct ggctaccatc aaactccatg tatttgcgtg aggttaactc
     3901 actatctgca ataacggtta gcttaccgtc cagtgatacc gtgttactgt tgccgctgat
     3961 attcacacca acggatgggt cgtagaaata atctaccgcg ttatctgcgg tttgatcttt
     4021 atccaccaga atattaccgg tgatatcaac ggtattactc tcgcctgaga tgttaacgcc
     4081 tgtcgctttc tgcccggtga cattgatatc tttcgccgcc agcgtcacgc tgttgttgtc
     4141 gccgctaagc gccatgcccg ttgaaaaatc gcccactttc atgctgccag ccagcgagat
     4201 aatcccctca ttggtaacga ggctgaatcc ggtagccgaa ttgctgacgt caatatcgcc
     4261 cgtattggtc aacgtcgcac tgtcgccatt gatggacaca ccaaccgagt cagtacctgt
     4321 gatggtcatt ttgcctttag tgtcgacctt ggcattgttg cctgtaatgt cgataccgcg
     4381 gccgccgctg ctaaccgtga gttcaccttc ctgagtaacg gtggcgttat tacctgcgat
     4441 ctcggtgccg gtggaatctt tgccgtcaac ggtggtgttg ccgttattgt tcacggtggc
     4501 ttcgtcgccg ttaacctgcg tgccggtgcc gccgttgctg atggcattgt caccgtcgtt
     4561 gttcacgacc gccttgtcgc cgtctatctg aatgccaatc gaatccgcat ccgcaacggt
     4621 catgccgcct ttgttgtcca ccgttgcgct gtcgccggtg atatcaatcc cgtgaccgcc
     4681 accgctcaca tccagctcgc cgtcctggtt caccaccgcg ttattacccg cgatttcagt
     4741 tccggtcgaa ccctgaccgt ctacggtggt attgccactg ttg
//
LOCUS       DAZFRE010000197         4600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFRE010000197 DAZFRE010000000
VERSION     DAZFRE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.202"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..593)
                     /locus_tag="ACHKUI_004955"
     CDS             complement(<1..593)
                     /locus_tag="ACHKUI_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6600967.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHKUI_004956"
     tRNA            complement(708..783)
                     /locus_tag="ACHKUI_004956"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHKUI_004957"
     tRNA            complement(790..864)
                     /locus_tag="ACHKUI_004957"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHKUI_004958"
     tRNA            complement(981..1065)
                     /locus_tag="ACHKUI_004958"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHKUI_004959"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHKUI_004959"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKUI_004960"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKUI_004960"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6600968.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKUI_004961"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKUI_004961"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6600969.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKUI_004962"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKUI_004962"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6600970.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNTGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1156 c         1046 g         1217 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gtattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac tgttgtagga tccagacgag
     3901 ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtcgcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 cacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tcctccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacgggttg tccttctgcg gttgcatgct
     4381 gccaggcatt gagtaattgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgatg acaggaagag
     4561 tttatagaca cgcaaaaagg cccagtcttc cgactgggcc
//
LOCUS       DAZFRE010000198         4433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFRE010000198 DAZFRE010000000
VERSION     DAZFRE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.203"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..623
                     /locus_tag="ACHKUI_004963"
     CDS             <1..623
                     /locus_tag="ACHKUI_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO6600971.1"
                     /translation="KSHLEQLDSVGSAAVVCHQGQLYAFITAAENLHPDDTDALLARV
                     RAQLAVQLPYYLLPQHFFLLKVLPMTGNGKIDQAAMVQEVIQRMSQSTSQKSRALAHA
                     SPYEQQVAALWCEVLQREQIGLNDNFFEAGGGSIQIVLLHRRIEEIFKVTVPIAELFR
                     LTTVKRIAGYLQAMQDNARAVNQTQQRDASRSRAQQRLVRRHQRQR"
     gene            634..>4433
                     /gene="clbK"
                     /locus_tag="ACHKUI_004964"
     CDS             634..>4433
                     /gene="clbK"
                     /locus_tag="ACHKUI_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin hybrid non-ribosomal peptide
                     synthetase/type I polyketide synthase ClbK"
                     /protein_id="HFO6600972.1"
                     /translation="MTYSESDIAIVGMNCRYPGVHSVAAFETVLRTGCNILDPKVTPS
                     NGHNHITLNNVYEHMAEFDANFFGYSRAEAEIMDPQQRVFLTCAWEMFEQSGYNPKQH
                     DARVGLYAGVSTSFYLLTHLMNNPDKLAQLGGLQIMVGNDKDHLTSQLAYRLNITGPC
                     VTVQASCATSLVAVHLACEGLLSGQCDMALAGGVTFRMEEQRSYESHGDGLQAEDGLI
                     HTFDAQASGTVYSSGLGMVLLKRATDAQVQGDNILAVIKGSAINNDGGARSGYTVPGV
                     DGQEAVMIEAHSLAEVTPQQIQYLELHGSGTPLGDAIEFAAIKRVFGTPAPNATPWRL
                     GAVKPNVGHVEMASGITSLIKTVLSLTNRVFYPTLNFQRANPQLGLEDSPFEVVSRLT
                     PWPEGTTPRTAGVSAFGLGGTNAHLVVQAPLSTPQARAQQMGPCVVVLSAKNHNALEQ
                     MQNALLAKLAAHPEIRLQDVAYTLRHGRFSAPVRKCVIAENCTQLARQLRDAPMVEAT
                     TGCTIYWRLGHRFVVALETLSDWLACSEVLSQAVGQLLEHFPLEPACLQDLSPAQRTF
                     ISQYALIALIDERETLNVVLCGDGDGGYAAAVLRGDCTLEQAWHRLNAGQPFDDVPTN
                     PLLQPDVCSLMLDDAASDANRTALEALGQLWLAGVSLDWRWVDAAERMLGSQRIALPG
                     TVFTPQRYWVEAVRPATFSHESSNNLLSRATKSDIIAVVTEIWERTLGVSIDDHHASF
                     FELGGHSLLASTILYDIQQRYGITCTLSAFFADPTIEGLSCYLLEQGGSETAVSALPD
                     TVFAPDQQHLPFPLTDVQQAYWVGRRKSLGLGNISTHIYVEYELQGLDETAFNRALNA
                     VIARHSMLRAIVNDDGMQQILPNVPEYHVAFYTTQCEDAFQQRCRELRDTLSHQMIDC
                     SRWPLFQMEVVVDPQQKARLHVSIDLLIADAWSLELFIRELAYHYRHPQAALPTLTYS
                     FRDYVLTLKSYEKTPQFERARDYWRARIETLPPGPRLPLRTDPTKLENPTFVRRSYCL
                     SRAIWQRLKTQAGQMSITPTTLLLTGFAQVLARFSSSPHFSLNLTLFNRLPLHADINH
                     LIGDFTALTLLEIDMSQGETLQARANVIHSQLWRDLDNRLFGGIQVSRLLVQTHRDPA
                     KSVIPIVFTSLLNQYEASWETDDTLFNQPQDDLYSISQTPQVWLDHQVMERNGELHFN
                     WDVVEQLFEPALMDQMFQCYCQLLHALAQRPQLWHETQDVLALPTVSAPVTQAPAPTA
                     LLHHG"
BASE COUNT          979 a         1183 c         1259 g         1012 t
ORIGIN      
        1 tcaaaagcca ccttgaacag ctcgacagcg tagggagtgc cgccgtggtg tgccaccagg
       61 gacagctgta tgcctttatc actgccgcag aaaacctgca tcctgacgat actgacgccc
      121 tgttagcgcg tgttcgtgct cagttagccg tgcagttgcc gtattacctg ctgccccaac
      181 atttctttct tctcaaggtg ctgccgatga caggcaacgg caaaattgat caggcggcaa
      241 tggttcaaga ggttatccaa cgtatgtcgc aatctacatc acagaagtcg agggccctcg
      301 cgcacgcctc gccctatgaa cagcaggtgg ccgctctctg gtgcgaggta ctacaacgag
      361 aacagatcgg actgaatgac aacttttttg aagcgggggg cggctcaatc cagatcgtgc
      421 tgttgcatcg ccgtattgag gagattttta aggttacggt acctattgct gagctatttc
      481 gcttaaccac cgtgaaaaga attgccggtt atttgcaggc tatgcaggac aatgcacggg
      541 cggtgaatca aacacagcag cgtgatgctt cccgatcccg cgcccagcaa cgacttgtac
      601 gtcgtcacca gcgtcagcgt taaggaaaaa ctaatgactt acagtgaaag cgatattgcc
      661 attgttggca tgaactgccg gtaccccggc gtgcacagcg ttgcggcatt cgaaacggtg
      721 cttcgcaccg gatgcaacat tttggacccc aaagtgaccc cgagcaacgg ccacaatcac
      781 attacgctga acaacgtcta tgagcacatg gcggagttcg atgccaactt ttttggctac
      841 agccgtgcag aagcagaaat catggatcca cagcagcgcg tatttctgac ctgtgcttgg
      901 gagatgtttg aacaaagcgg ttacaacccg aagcagcacg atgcgcgcgt cggcctatat
      961 gcgggcgtga gtaccagctt ctatttgctt actcacctga tgaacaaccc agacaagctt
     1021 gcacaactcg gtggtctcca aatcatggtc ggcaatgaca aagatcattt gacgtcccaa
     1081 ctggcttacc gcctgaatat taccggccct tgcgtgacgg ttcaggccag ttgtgccacc
     1141 tcgctggtgg cagtgcatct ggcctgcgaa gggctgctga gcggtcagtg cgatatggcg
     1201 ctagcgggtg gcgtcacgtt tcgcatggag gagcagcgca gctatgaatc gcacggtgat
     1261 gggctgcaag cagaggatgg cctgattcat acttttgatg cgcaggcgag cggtacggtc
     1321 tacagcagtg gtttgggcat ggtgctgctc aaacgggcta cagacgcgca ggtgcaaggg
     1381 gataacatcc ttgccgtcat caagggaagc gccatcaaca atgatggcgg tgcgcgcagt
     1441 ggttataccg tgccgggtgt tgatggtcag gaagctgtga tgattgaggc gcatagcttg
     1501 gcggaggtga cgccgcagca aattcagtat ctagaactgc atggcagcgg cacgccgttg
     1561 ggtgacgcta tcgaattcgc ggccatcaaa cgtgtgtttg ggacgccagc gccgaatgcg
     1621 acaccgtggc gtctgggggc tgtgaaacct aacgtgggtc acgtagaaat ggcatctggc
     1681 atcaccagtc tcataaaaac ggtgctgagt cttactaacc gggtgtttta ccctacgctg
     1741 aattttcaac gggctaatcc gcaactgggg ttggaggaca gcccgtttga agtggtgtcc
     1801 cgtttaacgc cttggccgga gggcaccacg ccacgcaccg ctggtgtgag tgcgttcgga
     1861 ttgggcggca ccaacgcaca tctggtggta caagcgccgt tatcgacgcc gcaggcgagg
     1921 gcgcagcaga tggggccttg tgtcgttgta ctctcggcaa aaaaccacaa tgccctggaa
     1981 cagatgcaaa acgccctgct ggcgaaactg gccgcacatc cagagatacg cttacaggac
     2041 gtggcttaca ccctgcgtca cgggcgtttc tccgccccgg tacgtaagtg tgtcatcgca
     2101 gagaattgca cccagctagc ccgacaactc cgcgacgcac cgatggtgga ggcaaccacg
     2161 ggttgcacga tctactggag attggggcac cgtttcgttg ttgcactcga gacgcttagt
     2221 gactggctgg cgtgctctga ggtgttgtca caggcggtgg gacaactgct tgagcatttt
     2281 ccgctcgaac cagcctgtct gcaagatctc tcccctgcgc aacggacgtt tatcagccag
     2341 tatgcgctga tcgcgttgat tgacgaacgg gaaacgttga atgtggtgct atgcggcgac
     2401 ggtgatggtg gctatgctgc cgccgtacta cgaggagact gcacactaga gcaagcatgg
     2461 cacaggttga atgcgggcca accgtttgat gatgtgccga cgaatcccct cttgcagccc
     2521 gatgtctgct ctttgatgct ggacgacgcg gcgagtgatg ccaatcgcac cgcgctagag
     2581 gcgttagggc agttatggtt ggctggcgtc agcctagatt ggcgctgggt ggatgcggcg
     2641 gagcgtatgt tgggcagtca acgcatcgcc ctgccgggga ccgtttttac accgcagcgc
     2701 tattgggtcg aggccgtacg gcctgccacg ttctcccacg aatcgtcgaa taatctctta
     2761 tcccgcgcaa caaaatccga tattattgca gtggtgacgg agatatggga acgcacgctt
     2821 ggtgtcagca ttgatgatca ccacgccagc ttcttcgaac tgggtgggca ttcgctgtta
     2881 gcgtcaacca ttttatatga tattcagcaa cgttacggca tcacctgtac gctaagcgca
     2941 ttctttgccg atcccaccat cgagggatta agttgctatc tgctcgaaca aggcggcagt
     3001 gagacagcgg tttcagcatt gcctgatacg gtctttgccc ctgaccagca gcacctacct
     3061 tttccgctga ctgacgtgca acaggcctat tgggttgggc ggcgaaaatc cttgggattg
     3121 ggcaatattt ccacccatat ctatgtggaa tatgaactac aggggctaga tgaaacagcg
     3181 ttcaaccgcg cgctcaatgc ggtgattgcg cggcacagca tgttgcgtgc aattgtcaac
     3241 gatgacggta tgcagcagat attgccaaac gtgccggaat accacgttgc cttctatacc
     3301 acccagtgcg aggatgcgtt tcaacagcgt tgccgtgagc tgcgtgacac cctctcacat
     3361 cagatgattg attgtagtcg ctggcctctg tttcagatgg aggtcgtggt tgatccgcag
     3421 caaaaagccc ggctacatgt atctatcgac ctgctgatag ccgatgcatg gagtctggag
     3481 ttgttcattc gggaactagc ttatcactac cgccatccgc aggcggcatt gcctacattg
     3541 acgtacagtt tccgcgacta tgtgctgacc ttaaaatcct acgagaaaac gccgcagttt
     3601 gaacgggcac gtgactactg gcgcgcccgc atcgaaactc tgccgccggg gccacgattg
     3661 ccgctgcgta ccgaccccac gaagctggaa aaccccacat tcgtgcgccg tagctattgt
     3721 ctatctcgtg ctatctggca gcgcttgaaa acgcaggcgg gtcagatgag tatcacgcct
     3781 actacactgc tgctcacggg gtttgctcag gtgttggcgc gctttagttc ctcgccgcac
     3841 tttagcctta acctgacgct gtttaatcgt ctgccgttgc acgcagatat caatcactta
     3901 attggcgatt ttactgcact gacgttgctg gagattgata tgtcgcaggg agaaaccttg
     3961 caggcgcggg caaacgtgat ccactctcaa ctgtggcgcg atttggataa ccgcttgttt
     4021 ggcggtatcc aggtctctcg actgctggta caaactcacc gcgatccggc gaaatcggtg
     4081 attcctatcg tgttcaccag cttgctcaat caatatgagg cgagctggga aacggatgat
     4141 acgctgttca accaaccgca ggatgatctg tacagtattt cacagacacc gcaggtgtgg
     4201 ctcgatcatc aggtaatgga gcgtaacggg gaattgcact ttaattggga cgtggtggaa
     4261 caattgtttg aaccggcgct aatggatcag atgttccagt gttattgcca actcctgcac
     4321 gccctagccc agcgaccaca gctctggcat gagactcaag atgtgctggc gctgcccacc
     4381 gttagtgcac cggtcacgca ggctcctgca cctacggcct tgttgcacca tgg
//
LOCUS       DAZFRE010000199         4430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFRE010000199 DAZFRE010000000
VERSION     DAZFRE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.204"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1172)
                     /locus_tag="ACHKUI_004965"
     CDS             complement(<1..1172)
                     /locus_tag="ACHKUI_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6600973.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSYPSLASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGRNGAVGGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAG
                     AYAWKTGGTVGSPLISDWSLVSNPGYLYQSVNGPLASYGTPGDSGSPLFAWDAVKKQW
                     VLVAVLNGYAGEKGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYNKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRP"
     gene            complement(1544..2017)
                     /locus_tag="ACHKUI_004966"
     CDS             complement(1544..2017)
                     /locus_tag="ACHKUI_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600974.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYLGLGKNGLKYRSVKHLVGRA"
     gene            complement(2104..2628)
                     /locus_tag="ACHKUI_004967"
                     /pseudo
     CDS             complement(2104..2628)
                     /locus_tag="ACHKUI_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
     gene            2774..3864
                     /locus_tag="ACHKUI_004968"
     CDS             join(2774..3314,3314..3864)
                     /locus_tag="ACHKUI_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="HFO6600975.1"
                     /translation="MTHLNELYLILNKYLKWNKSHLKCFALIMLVIILKQTCNLSSAS
                     KALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRN
                     INILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREF
                     IGEQWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVG
                     RVKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLT
                     DPRRVKKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQ
                     RLIGFGKKEEFKEILAILRRQNPDRIRVL"
     gene            complement(3806..4291)
                     /locus_tag="ACHKUI_004969"
     CDS             complement(3806..4291)
                     /locus_tag="ACHKUI_004969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6600976.1"
                     /translation="MSKKFTKTLLSSSLVGLLVVSAEGFAQASVYPKDSQYSVLVDDA
                     KQDVFGSKFKVYYSKSSHRGVVRYVQNGKDNVLFHFGDDGNILVLSKDGQIGYTVHEP
                     VLKDFARMAEGLKVGKGHKLELDEAEVQRIYNKVSLYTTNFTEPLSYQGSAFLKLPKF
                     P"
BASE COUNT         1207 a         1157 c          868 g         1198 t
ORIGIN      
        1 ggtctgaccg tataatcacc attaaacgtg agcgaacccg ccccctggac aacatcatcc
       61 ttcagaacaa ccgtgccctt acccccaaag gtgatatctt tcccgtcatt cagactccct
      121 cccctgaagc cgttcatctt ccataccttc ccgtcctgag aaagggttcc ctctccgctt
      181 cccttattgt aattccacac aatgtcacca ccggcaactg ccggcacgac agtgcctgaa
      241 ctgtcctgct ttatcgtgtt attcacatcc cctgcgggaa tcaccgtaaa ccagttcgtc
      301 ttccctttct caccggcata accattaagt acagcaacaa ggacccactg cttcttcacc
      361 gcatcccagg caaaaagcgg tgagccactg tctcccggtg tcccgtaaga tgccagcgga
      421 ccattgacac tctggtacag ataccccggg tttgacacca gactccagtc agaaatgagc
      481 ggacttccca ccgtcccgcc cgtcttccag gcgtaagcac ccgctattct ggtcagcgca
      541 ccggaagcag ctccggtgta ctgcgttcca ctccccatac gataaaacac cggataacga
      601 ctgcggttct ggtatacccc cttgccggtt ccggcatccg tgacgctcac cggggcaact
      661 tctgtcacca gtttgttcag ccggggagta tgaaaatcac gccagtgctc attacggtca
      721 acaaggcgat atgtcgtatc atccgcataa ccaaaacgca ccgtgttgta gctgccgtta
      781 tgcttaaccc ccgccacata ctgcggagaa atcagtgttg ccacccccag tatctggtcc
      841 acaacactga aatccatcat cggcgccttg tccagccgcc caccgacagc cccgttcctg
      901 ccataaagcg gaatatttgt ggcaccggca tgaaacgcgc ccttgttctc agcaaaatcc
      961 ctgtaggtct gccaggggag ggtattcccg acaacggatg ccagcgacgg ataagaaaac
     1021 agaacaccgg caacggcacc agaggccaga agaagacggg aaagcatccc cgcaccggga
     1081 atgccggacg actttttcct gccccccacc gtttccggta cagcaaccag ttcttttctg
     1141 tcattaactc tgagtgagta tattttgttc attccctggg attccctgta ttttctcccg
     1201 gacaacaata aacagcgggc acaatcatca tactaaaacg aactttggct gataaagatg
     1261 aaattaacat taacaaattg atttaaatat ataaacccac gagcatttaa caagttctca
     1321 gttatcagaa agttcgaatt aaagaagtaa actctcttcc gttttcacct cattatcaga
     1381 gcggccagca cacagaatgt caccacaccc cccagccata caaacagcat gaagcacatc
     1441 gcacccgctg tgtatttccg ctttccgcat ctgatttttt tctgttccat tctcactgcc
     1501 accgttcaac tctcattacc cgactcacga atattcattc cggctacgca cgtccaacca
     1561 ggtgcttcac actcctgtac ttaagaccgt ttttaccaag ccccagatac agaaaggtgt
     1621 tcaccgtctt cgtgtttatc cccatttcac gggcaagatg gcagggcttt gactcccggt
     1681 acacgtatcc aaacatcacc accatctcgg acatcgtcag aggtcgcgca tacgtattat
     1741 gtacccactc accattccgg attttcctgt acgtctccag gcgccccgta acatcttccg
     1801 catcaatctg aagataacgg cgtccctgaa acatgcagca catcagcctg ccaaggaggg
     1861 catcacagat tatcacccat gactcatcat gtgccggtac gctcttcctg ttctctggtg
     1921 taaaaaactc cctcgccagt attatcctgt acccctcgag catctgctgc aggccatact
     1981 ccagataacg gttgcgggtc gccagaatca ctttcatcgc tgtcacctgt ttcatgctca
     2041 catgagaaaa aacggcaggc aaaactccac gcctgccgtc atgcttcatg tcactgtcag
     2101 caattaaaac tcgaagttaa cgcccatgtt gtaggacaca tcgccatcac tcgctgcaat
     2161 acccgctttc gctgcggtct gctcgttgaa acggtaacca acacctacag caatcgcctg
     2221 tttatcgctg taaccaccca gggctgccgt ggcgttgaat ttccccacac tgtacggctg
     2281 gaaaagaccc gccagcgctg cactctgtgc tgctgcacgc ttcatctctt cgtggttttc
     2341 acgaatctgg cgctgctggc tgttcaggcg tgcattatgc tcctgcaact ggcgagtatt
     2401 ggactgaata gcctgtgtat ttttagtaac ccggcggtca acgctgtctg ttctgccgga
     2461 cagagcaacg agaccgtcat taacagcaat cgtttttgta gcgccatcag cgctggtcag
     2521 ggttccgtcg gacttaacac caactgtcgt tctggagtct gtatattcac gagacttttt
     2581 cgcttcaacc acaaggcggg tattcgtatc ctgagccagc tggtcaacgc gaccagaaag
     2641 ctcaccaact tgatttttca atgcaggatt tacaacagtg ttagacatgt tataaacttt
     2701 atctctgtac acgacaaaaa tagataactc attgaaataa tgtcataata attgttttct
     2761 aacgacgaat actatgacac atctcaatga gttatatctt atcttaaaca aatatctaaa
     2821 atggaacaag tcacatttaa agtgctttgc gctcatcatg cttgtgatta ttttaaagca
     2881 aacatgtaat ctttcttctg catctaaagc cttgcccatc aagtgcttac cacaatcatt
     2941 ttatcgacgt atgcagcgct tctttgcagg tcagtatttt gattatcgtc aaatttctca
     3001 gttgattttc aatatgtttt cattcgacca agtgcaactg actttagata gaaccaattg
     3061 gaaatgggga aaacgaaata ttaatatcct gatgctcgca atcgtttatc gtggaatagc
     3121 gatacctatc ctttggacat tgcttaataa acgtggaaat tcagatacga aagagcgtat
     3181 tgctttgatt caacgcttta tagccatttt tggtaaagac cgtattgtga atgtgttcgc
     3241 agacagagag tttatcggtg agcagtggtt tacatggtta attgaacaag acatcaactt
     3301 ctgcattcgt gttaaaaaaa cttcattgtc accaatcatt taggaaagaa tcataaaatt
     3361 agtgatttat ttcgccatct taaagttggt caaattgaat gtcgtaaacg acggattttg
     3421 gttggtcggg tgaaactata tataagtgca ctacagttag aaaatggaga gcttttactc
     3481 gtcgtttctc ctcagtttaa tgccaatgct attcaggatt atgcattacg ctgggaaatt
     3541 gaaaccttat tcagttgtct caaaggacgc gggtttaatc ttgaaaatac gcgcttgaca
     3601 gaccctagac gagtgaaaaa attgattgcg gtgttagcta taagcttctg ttggtgttac
     3661 ttaacgggtg aatggcaaca tgatcaaaaa aaagcgataa aaataaagaa gcatggacga
     3721 ctctcaatga gtttatttcg ctatggttta gactatgttc aaatggcgat tcagcgttta
     3781 attggttttg ggaaaaaaga agagtttaag gaaattttgg caattttaag aaggcagaac
     3841 cctgatagga taagggttct gtgaaatttg tcgtgtacag agaaacttta ttgtaaatac
     3901 gctgtacttc agcctcatcc agctccagct tatggccttt accaacttta agaccttcag
     3961 ccatacgggc aaagtccttc aacacaggct catggacggt atatcctatt tgcccatcct
     4021 tgctcagcac caaaatattg ccgtcatcac cgaagtgaaa tagaacatta tcttttccat
     4081 tttgcacata tcgcacaact ccccggtgag atgacttact gtaataaact ttgaatttgc
     4141 taccgaatac atcctgtttc gcgtcatcca ctaaaacgct atactgcgag tctttcggat
     4201 atactgatgc ctgtgcgaaa ccctcagcac tcacaaccag aagccctacc agagaagatg
     4261 acagaagtgt ctttgtgaat tttttactca ttatggatat cctgttaaat tatctgtata
     4321 caaatcagcc gcaacacctc acactcataa agaaatatga ttaattacga cctcattcag
     4381 gatattccga aaataaattt aaaaattaat ttaataactt taggatttgt
//
LOCUS       DAZFRE010000200         4313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFRE010000200 DAZFRE010000000
VERSION     DAZFRE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.205"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            44..4173
                     /gene="vat"
                     /locus_tag="ACHKUI_004970"
                     /pseudo
     CDS             44..4173
                     /gene="vat"
                     /locus_tag="ACHKUI_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
BASE COUNT         1196 a          981 c         1086 g         1050 t
ORIGIN      
        1 ttattaatgt aactttggaa tatacgttcc ggaatcattt actatgaata aaatatacgc
       61 tctaaaatat tgttatatta ctaacacagt aaaggttgtc tctgaactag cccgaagggt
      121 atgtaaaggg agtacccgca gaggaaaaag actttcagta cttacctctc tggcactatc
      181 tgcattactc ccaaccgttg ctggtgcatc aacggttggt ggcaacaatc cttaccagac
      241 ataccgcgac tttgcagaaa acaaagggca gtttcaggct ggcgcaacaa acattcctat
      301 ttttaataat aaaggggaat tagtaggaca tcttgataaa gcgcccatgg ttgattttag
      361 cagtgtgaat gtaagctcaa atcccggcgt tgcaacatta attaacccgc aatatatagc
      421 cagtgtaaaa cataataaag gatatcagag cgtcagcttc ggtgatggtc agaacagtta
      481 ccatattgtg gatcgtaatg aacacagttc atctgatctc cacacaccaa gacttgataa
      541 gctcgtaact gaggttgctc cggctaccgt aaccagctca tcaacagctg atatattgaa
      601 cccttcaaaa tactcggcat tctacagggc tggttcggga agtcagtata ttcaggatag
      661 tcagggtaag cgacattggg taacaggtgg gtatggttat ctgacaggag gaatactccc
      721 gacatcattc ttttatcacg gctcagacgg cattcagctg tatatggggg gcaacataca
      781 tgatcatagc atcctgccct cttttggaga ggccggcgac agtggttctc cattatttgg
      841 ctggaatacg gccaaagggc agtgggaact ggtcggtgtt tactcgggag taggaggggg
      901 gaccaatttg atatattctc ttattcctca gagttttctc tcacagatct attcagagga
      961 taatgacgct cccgtctttt ttaatgcctc atccggcgcc cccctgcaat ggaaatttga
     1021 cagcagcacc ggcactggct ctctgaaaca gggttccgat gaatatgcca tgcacgggca
     1081 aaaaggttct gacctgaacg caggtaaaaa tctgacattc ctgggacata atggtcagat
     1141 tgacctggaa aactctgtca cgcagggtgc cggttcactg acatttactg atgactacac
     1201 tgtcaccact tcaaacggaa gtacctggac cggggccggt attattgtgg acaaggatgc
     1261 ctccgtaaac tggcaggtta atggtgtgaa aggtgacaac ctgcataaaa tcggcgaagg
     1321 aaccctggtt gtacagggaa ccggtgttaa tgagggcggc ctgaaagtcg gggatgggac
     1381 cgttgtcctc aatcagcagg ctgacagttc aggacacgtt caggcattca gtagcgtgaa
     1441 tattgccagc ggccgcccga cagtcgtgct ggcagacaac cagcaggtta atccggacaa
     1501 tatatcctgg ggctaccggg gggggttctg gatgttaacg ggaatgacct gacatttcat
     1561 aagctgaatg ccgccgatta tggcgcaact ctcggtaaca gcagtgataa aacggctaat
     1621 atcactctgg attatcagac gcgtccggca gacgtaaaag ttaatgaatg gtcatcatca
     1681 aacaggggaa cagtaggttc attatatatt tataataatc cctatactca taccgtcgat
     1741 tattttatcc tgaaaacaag tagttatggc tggttcccta ccggtcaggt cagtaacgag
     1801 cactgggaat atgtcggaca tgaccagaac agtgcacagg cactgcttgc aaacagaatt
     1861 aataataaag ggtatctgta tcatggcaag ttgctgggaa atattaattt ctcaaataaa
     1921 gcaaccccgg gtacaaccgg cgcattggtt atggacggct cagcgaatat gtccggtaca
     1981 tttactcagg aaaacggtcg tctgaccatt cagggccacc cggttatcca tgcttcaacg
     2041 tctcagagta ttgcaaatac agtctcgtct ctgggcgaca attccgttct gacacagccc
     2101 acctcattta cacaggatga ctgggagaac aggacgttca gctttggttc gctcgtgtta
     2161 aaagatacag actttggtct gggccgcaat gccacactga acacaaccat ccaggcagat
     2221 aactccagcg tcacgctggg cgacagtcgg atatttatcg acaaaaaaga tggccaggga
     2281 acagccttta cccttgaaga aggcacatct gttgcaacta aagatgcaga taaaagtgtc
     2341 ttcaacggca ccgtcaacct ggataatcag tcagtgctga atatcaatga tatattcaat
     2401 ggcggaatac aggcgaacaa cagtaccgtg aatatctcct cagacagtgc cattctgggg
     2461 aactcaacgc tgaccagtac cgccctgaat ctgaacaagg gagcaaatgc tctggccagt
     2521 cagagttttg tttctgacgg tccagtgaat atttctgatg ccaccctgag tctgaacagc
     2581 cgtcctgatg aggtatctca cacactttta cctgtatacg attatgccgg ttcatggaac
     2641 ctgaagggag acgatgcccg cctgaacgtg gggccgtaca gtatgttgtc aggtaatatc
     2701 aatgttcagg ataaagggac tgtcaccctc ggaggggaag gggaactgag tcctgacctg
     2761 actcttcaga atcagatgtt gtacagcctg tttaacgggt accgcaatac ctggagcggg
     2821 agcctgaatg caccggatgc caccgtcagc atgacagaca cccagtggtc gatgaacgga
     2881 aactccacgg caggaaatat gaaacttaac cggacaatag tcggttttaa cgggggaaca
     2941 tcaccgttca cgacactgac aacagataat ctggacgcgg ttcagtcagc atttgtcatg
     3001 cgtacagacc ttaacaaggc agacaaactg gtgataaaca agtcggcaac aggtcatgac
     3061 aacagcatct gggttaactt cctgaaaaaa ccctctgaca aggacacgct tgatattcca
     3121 ctggtcagcg cacctgaaac gacagctgat aatctgttca gggcatcaac acgggttgtg
     3181 ggattcagtg atgtcacccc cacccttagt gtcagaaaag aggacgggaa aaaagagtgg
     3241 gtcctcgatg gttaccaggt tgcacgtaac gacggccagg gtaaggctgc cgccacattc
     3301 atgcacatca gctataacaa cttcatcact gaagttaaca acctgaacaa acgcatgggc
     3361 gatttgaggg atattaacgg cgaagccggt acgtgggtgc gtctgctgaa cggttccggc
     3421 tctgctgatg gcggtttcac tgaccactat accctgctgc agatgggggc tgaccgtaag
     3481 cacgaactgg gaagtatgga cctgtttacc ggcgtgatgg ccacctacac tgacacagat
     3541 gcgtcagcag gcctgtacag cggtaaaaca aaatcatggg gtggtggttt ctatgccagt
     3601 ggtctgttcc ggtccggcgc ttactttgat ttgattgcca aatatattca caatgaaaac
     3661 aaatatgacc tgaactttgc cggagctggt aaacagaact tccgcagcca ttcactgtat
     3721 gcaggtgcag aagtcggata ccgttatcat ctgacagata cgacgtttgt tgaacctcag
     3781 gcggaactgg tctggggaag actgcagggc caaacattta actggaacga cagtggaatg
     3841 gatgtctcaa tgcgtcgtaa cagcgttaat cctctggtag gcagaaccgg cgttgtttcc
     3901 ggtaaaacct tcagtggtaa ggactggagt ctgacagccc gtgccggcct gcattatgag
     3961 ttcgatctga cggacagtgc tgacgttcac ctgaaggatg cagcgggaga acatcagatt
     4021 aatggcagaa aagacggtcg tatgctttac ggtgtggggt taaatgcccg gtttggcgac
     4081 aatacgcgtc tggggctgga agttgaacgc tctgcattcg gtaaatacaa cacagatgat
     4141 gcgataaacg ctaatattcg ttattcattc tgatgctgtg cagcaaaggg actattgctt
     4201 tgtccctttg ctgcgattat ctgattacgg tacaggctgg aacttagatt ttccggcgtt
     4261 cacgatactt catgtaacac tcaggttgag taatcttcat gagttttctt ttg
//
LOCUS       DAZFRE010000201         4143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFRE010000201 DAZFRE010000000
VERSION     DAZFRE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.206"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            129..1787
                     /gene="dpiB"
                     /locus_tag="ACHKUI_004971"
     CDS             129..1787
                     /gene="dpiB"
                     /locus_tag="ACHKUI_004971"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6600977.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1756..2436
                     /gene="dpiA"
                     /locus_tag="ACHKUI_004972"
                     /gene_synonym="citB"
     CDS             1756..2436
                     /gene="dpiA"
                     /locus_tag="ACHKUI_004972"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6600978.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2477..3862)
                     /gene="dcuC"
                     /locus_tag="ACHKUI_004973"
     CDS             complement(2477..3862)
                     /gene="dcuC"
                     /locus_tag="ACHKUI_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6600979.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1141 a          960 c         1056 g          986 t
ORIGIN      
        1 caggtttaag tgatttaatt gatttaatga ataaaatttg ccacgatcat atttaatatc
       61 tatgtatttt gattcaacat tttaattaca tccgtcaaag aggctcggga caacccgcaa
      121 ggaaaacaat gttgcagctt aaagagaata aacagtttgc atttttccaa agactggcat
      181 ttccgctgcg tatctttttg ctgattctgg tgttctcaat atttgtcatt gcagccctgg
      241 cgcaatattt tacagccagt tttgaggact atttaacgct tcatgtgcgc gacatggcaa
      301 tgaatcaggc aaaaattatt gcttctaatg acagtatcat cagtgcagtg aaaacgcgtg
      361 actacaagag gctggcgacc atcgctgaca aattacaaag agatactgat ttcgattatg
      421 tggtgattgg cgatcggcac tcgattcgtc tttaccatcc taatccggag aaaattggtt
      481 atcctatgca gttcaccaaa ccgggcgcgc tggagaaagg ggagagctac ttcatcaccg
      541 ggaaagggtc aattggcatg gcgatgcgtg ccaaaacgcc aatctttgat gacgatggaa
      601 aagtcatcgg cgtggtgtcg attggctacc tggtgagtaa aatcgatagc tggcgggctg
      661 agtttttatt accgatggca ggcgtgtttg tcgtgctgtt agggattttg atgttgctgt
      721 cgtggttcct ggccgcgcat atccgtcggc agatgatggg catggagcca aagcaaatcg
      781 ctcgcgtagt ccgtcagcaa gaggcgctgt ttagttcggt ttatgaaggg ctgattgcgg
      841 tggatccgca cgggtacatt accgccatca atcgtaacgc aagaaagatg ctggggctga
      901 gctcccccgg acggcaatgg ttgggtaaac ccattgctga agtggtcagg cccgccgatt
      961 tctttaccga acagattgat gaaaaacgtc aggatgtggt ggcgaacttt aacggtctga
     1021 gcgttattgc caaccgggaa gctattcgtt ctggcgatga tttactgggg gccattatta
     1081 gctttcgtag taaagacgaa atttccaccc tcaatgcgca actgacgcaa attaaacaat
     1141 acgtcgagag ccttcgtaca ttgcgacacg agcatctcaa ttggatgtcg acgctcaatg
     1201 gtctgttgca gatgaaagag tatgatcgcg tgctggcgat ggtgcagggg gagtctcagg
     1261 cccagcaaca gcttattgac agcctgcgtg aggcgtttgc cgatcgccag gtggcggggc
     1321 tgctttttgg taaagtgcag cgcgcccgcg aactggggct aaaaatggtc atcgtcccag
     1381 gtagccagct ttcgcaactg ccgccaggac tggacagcac cgagtttgca gccattgttg
     1441 gcaatttact tgataacgcc ttcgaagcaa gcctgcgtag cgatgaagga aacaagatcg
     1501 ttgaattatt cctcagcgat gaaggcgatg atgtggtgat tgaagtcgcc gatcagggct
     1561 gcggcgttcc agagtctcta cgagacaaaa tatttgagca gggtgtcagt acgcgtgctg
     1621 acgagcccgg tgaacatggc attgggttgt acttgattgc cagctacgta acgcgctgcg
     1681 gtggtgttat cactctcgaa gataatgatc cctgcggtac cttattttca atctatattc
     1741 cgaaagtgaa acctaatgac agctccatta accctattga tcgttgagga cgaaacgccg
     1801 ctggcagaga tgcatgcgga atatattcgt cacattcccg gattcagcca gatattactg
     1861 gcgggaaacc tggcgcaggc ccgaatgatg atcgagcgtt ttaagccggg gctaatcttg
     1921 cttgataact atcttcctga cggtagaggg attaatttac tgcatgaact ggtgcaggcg
     1981 cattatcccg gcgacgtggt gtttaccact gcagccagcg atatggaaac ggtgtctgaa
     2041 gccgtacgtt gtggtgtatt tgattatctc attaagccca ttgcctatga acggttgggg
     2101 caaacgctaa cccgtttccg ccagcgtaaa catatgctgg aaagtattga tagcgccagc
     2161 cagaagcaaa ttgatgagat gtttaatgcc tatgctcgcg gtgaacccaa agacgaactg
     2221 ccgaccggca ttgacccctt gacgctaaac gcggtgcgaa aactgttcaa agagcctggt
     2281 gtgcaacata cggcagaaac ggtggcgcag gcactgacca tcagccgcac cactgccagg
     2341 cgttatcttg aatattgcgc cagccgccat ctgattattg ctgaaattgt tcacggcaaa
     2401 gttggcagac cgcaacgcat ataccacagt gggtgacatg ataaagccgg aggaaacttg
     2461 cctccggcat tactatttac ttgcctgtga ccgctgctgc cgttcctggc accatcagct
     2521 ctgtagcaac gataacaatc accaaaccaa caagcaccgg caccgaggtg cgttttacga
     2581 cttcaaacgg cgagatcttc gccatcccgg caaccgcaac gactacgcca gaaacgggcg
     2641 aaagggtacg gcccaggttt gacgcctgca gcatcgggat agtcaaatac gccgggttaa
     2701 tgccggaaga gtgcgccagt ttcgggatca tctcaacaaa cgcataaaac ggcgcattgc
     2761 ctgaaccggt cgtgactgcc gccagcattg tcaggatcac caataccagc atcaggatga
     2821 tactcgccga accaaacgag gtagcgatag aaatcagact ttgaataaag ccgatggtgc
     2881 taagcccctg agcgaatacc ccagcggcaa ccagcagcat caccacgtta gcaaatgcat
     2941 ctgccatacc gcgataagcc acttccagac cagagaaaac tttctgggta ttaaagctgc
     3001 ggatgaactc cagaatggag gcaattagca tacaaatcac cagaatagtg atgatgtgta
     3061 attgcggacc ccatttgccg tcaaaaatca gcactccgat gatcggcgtg aacggcaaaa
     3121 tggcataaaa cgcaggggca gtggtggtga tttcactgac atctaacatt tcatgagaga
     3181 tgtgctcttt tttatccaga taacgttgcc agaagaagtg ggcgattgcc atgccgataa
     3241 ttgcagcaat tgagataggc agcgttgttt tgaaggcgaa gtcaatcagc gacatttcgg
     3301 aagcctgcgc cgccagcacc acatctcctg aagtcggtgc gagaataatc gccgccgggg
     3361 aggcacaaat ggcagcagct gcgccacgac tgataccaac gtttaccatc accggaaaca
     3421 gggttgccat cagcaaaaca cccagaccgg ttgcggaaga gacagccagt gacatcagac
     3481 aggcaacaaa ataggcggca atcatcagaa ggtaagggga gttaatatac tgcaatggtt
     3541 ttgacgccag cttgaccacc atatcattcg cgccgatatg ggtcatgtaa gcggcaaagc
     3601 cacacagcat cataatcatc atgccgaggt cgccgccgcg gctcattagc aatattttaa
     3661 cgtattcaac gatatccgtg gcgctgtagc ctgttgaagc ctggctggac ggtaacactt
     3721 tgtgccccat aatggcactg ataatcagca ataacaggcc accgacaaat aacacgccag
     3781 tggccgaata ccctttaatg atgtagcgag ctacacccac aataaccaca accccaataa
     3841 ggagttcaat gaatgtcagc ataatttttc ctgtctccag gccccaaagt aaataataaa
     3901 aaattcctaa aagttaagga aaaaatatgc ccaataaatt ggcggtgaat gctgattaaa
     3961 atcaagaaaa actgccatta agacattgaa gttgctgttt ttatgcgtag ataacaaaac
     4021 gcatatttag cggttggcaa atatcacata ataatgtgtg taaattgatg gtggattgat
     4081 gcaaatttgt taattgtaag gtgttagctg cggttgtgtg ctcaaaaaat aatcttccat
     4141 gag
//
LOCUS       DAZFRE010000202         4127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFRE010000202 DAZFRE010000000
VERSION     DAZFRE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.207"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            53..847
                     /gene="hyxR"
                     /locus_tag="ACHKUI_004974"
     CDS             53..847
                     /gene="hyxR"
                     /locus_tag="ACHKUI_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO6600980.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1001..1762
                     /gene="hyxA"
                     /locus_tag="ACHKUI_004975"
     CDS             1001..1762
                     /gene="hyxA"
                     /locus_tag="ACHKUI_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO6600981.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHNWEYSAQFLKTGIRQIWLTEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..4101
                     /gene="hyxB"
                     /locus_tag="ACHKUI_004976"
     CDS             1804..4101
                     /gene="hyxB"
                     /locus_tag="ACHKUI_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO6600982.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTDSVTLNKDKTT
                     LDQDVVINNTADKQITAVTINAANEDLNVTFAGHDITAESTADKKFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVISSTGDVVVNGGNFVAKNEKGSATGISLEATTGNN
                     LTLNGTTINAQGNKSYSNGSTAIFAQKGNLLQGFDGDATDNITLADSNIINGGIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGAGSATQNLNLSVADSTIYSD
                     VLALSESENSASTTTNVNMNVARSYWEGNAYTFNSGDKAGSDLDINLSDSSVWKGKVS
                     GAGDASVSLQNGSVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWKDGNGLFIQPYAKLSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
BASE COUNT         1132 a          893 c         1047 g         1055 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggttttgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt cactaggttg gcactggagt ctctcatccc
      121 aacggacagg tatatcgtgc gggtgtacag caacgtcact acagaggttg aattcgtttt
      181 aaagacctcc tgtggctacc tgttagctga tatcccttcg cttccttcag gggagttggt
      241 tcttctggct tgtctcgccc gtttgcggag tgatgccggg caatggcagg tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgctgcaagt ctgtataacg gcttcccacg
      361 catcggcacc atgctgacag cgagtcgtta tcagaatcaa attatccgct ggttactgcg
      421 tcctttgccc ggcgatatga gcgatttctg gctcctgact gcacgggagt tgagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagaccctg catgcgattg ccacacgggc attaatgaaa cttggactgg gaactctgag
      601 cgatttcagg ctgctctata cgggatgcgg tgacagcaga gtgaaacgga atatacggct
      661 acatgcaaga tatgtcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtatccgga attctttatc cagagaaagt tgccgttggt gaatcaataa attctgacgc
      781 catatccgga aaagtacttt ccggacaaag aaaaaatatg tctaaaaaaa gaccaggatt
      841 gttatgacac agcagcgttg ttactacccc ccactgcggc gcatacggac tcgctacagt
      901 gtaggagcca aaatatttct actgctctgg gctgcaggat tgggaggcac gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga attgtaccag cgcagttcgc agctctatca accggttctg tcggcaatgt
     1081 tacctgcatc agaacagaag aggagttacg caataaattg cggcataggc cacagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatcggga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccaccgg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga atgtcagtag agcgtgtcat
     1321 cagtattgca aatgcctctg gtgggttttc agggctgaaa catgcagaaa ttttgccagt
     1381 tatggacagt tactctgtct ttatgaaaga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg ttacttcttg ccggacacaa
     1501 ctgggaatac agtgcgcaat ttctcaaaac ggggataaga cagatatggc ttaccgagca
     1561 gtcgctaaaa aagcgttggg gaataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aaatttcacg gtgatggaaa cgcattggaa acaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtggaaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaagc attttaccgc agcgcattag caacagctat tgttatggct
     1861 ctttctgcac cagcattcgc tgctgataac gcggtatcaa ctgattcggt tacgctgaat
     1921 aaagataaaa cgactctgga tcaagatgtt gttattaaca atacagcaga caaacagatt
     1981 acagccgtaa caattaatgc ggcaaatgaa gacctgaatg ttacttttgc tggtcatgat
     2041 attactgctg aatcaacggc agacaaaaaa ttcgttgaag gtgtaaaagt tagtggtaac
     2101 aaaaatgttg tgattaatgc tacagactcc accatcacag ctcaaggtga aggcacctat
     2161 gtccggactg caatggtcat ttcttcaact ggcgatgttg ttgttaatgg cggtaatttc
     2221 gttgcaaaaa atgaaaaagg tagtgcgaca gggatatctc tggaagcgac cacgggaaat
     2281 aatttaacgc tcaatggtac aaccataaat gctcaaggta ataagagtta cagcaacggc
     2341 tctacggcaa tttttgctca aaagggtaat ttgttgcagg gttttgacgg tgatgcaacc
     2401 gacaacatca cccttgctga ctcaaatatt attaatggcg ggattgaaac aatagttact
     2461 gccgggaata agacgggaat tcatacagtc aacctgaata ttaaggatgg ctcagtaatt
     2521 ggggcggcta ataataaaca aacaatttat gcctctgctt cggcacaagg cgcaggttca
     2581 gcaacgcaaa atttaaattt gtctgttgct gattcaacca tctactctga tgtcctggcc
     2641 ctttctgaaa gcgagaattc agccagtacc acaacaaatg taaatatgaa cgttgcccgc
     2701 tcttactggg aaggtaatgc ttataccttc aatagcggcg ataaagcggg tagtgatctg
     2761 gatataaatc tttccgatag ttcagtctgg aaaggcaaag tttcaggggc aggagatgcc
     2821 agtgtatctc tgcaaaacgg gtctgtctgg aatgttacgg gttcctcaac tgttgatgct
     2881 ctggcagtaa aagacagtac ggttaatatc acgaaggcta cagtcaatac tggcacgttt
     2941 gcttctcaaa acggcactct gattgttgat gcctcttctg aaaacactct ggatatcagc
     3001 ggtaaagcga gcggtgactt gcgtgtttac agtgcgggtt cattggatct tatcaatgaa
     3061 caaacggcat ttatttctac cggcaaagac agcactctaa aagccacagg cacaacggaa
     3121 ggtggtctgt atcaatatga cctgacacag ggagctgatg gtaactttta tttcgtaaaa
     3181 aacacgcata aagcatccaa cgccagctcc gtgattcagg caatggcagc tgctccggct
     3241 aacgtcgcta atctgcaggc tgacacgctc tccgcccgtc aggatgctgt ccgtctgagc
     3301 gaaaatgaca agggtggcgt atggattcag tactttggcg gtaaacagaa acataccacc
     3361 gcgggaaatg catcctatga cctggatgta aatggtgtaa tgctgggtgg tgatacccgc
     3421 ttcatgactg aagatggtag ctggctggcc ggtgtggcga tgtcttctgc gaaaggtgac
     3481 atgactacca tgcagagcaa aggtgacact gaaggttaca gcttccacgc ttacctgagc
     3541 cgccagtata acaacggtat cttcattgat actgctgcac agtttggtca ctacagcaac
     3601 acggcagatg ttcgcctgat gaatggtggc ggtaccatca aagctgactt taacaccaat
     3661 ggttttggtg cgatggttaa aggcggttac acatggaaag acggtaatgg cctgtttatt
     3721 cagccatatg ccaaactgtc tgctctgact ctggaaggtg tggattatca actcaacggc
     3781 gtggacgttc attctgacag ctataactct gtgctgggtg aggccggtac gcgcgtgggt
     3841 tatgacttcg ctgtgggcaa cgcgaccgtt aaaccttatc tgaatctggc cgcactgaac
     3901 gaattctctg atggcaacaa agtccgtctg ggtgatgagt ctgtcaatgc cagcattgac
     3961 ggtgcagcat tccgcgtggg tgcaggtgta caggctgata tcaccaaaaa catgggagca
     4021 tatgcaagcc ttgactacac caaaggtgac gacattgaga acccgctaca gggtgtagtt
     4081 ggtatcaatg tgacctggta agaataaagc agtcggttcc cctgtcg
//
LOCUS       DAZFRE010000203         4091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFRE010000203 DAZFRE010000000
VERSION     DAZFRE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.208"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(46..747)
                     /locus_tag="ACHKUI_004977"
     CDS             complement(46..747)
                     /locus_tag="ACHKUI_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6600983.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTE
                     LQKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1570)
                     /locus_tag="ACHKUI_004978"
     CDS             complement(1280..1570)
                     /locus_tag="ACHKUI_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFO6600984.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PGETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
     gene            1741..3888
                     /gene="mobQ"
                     /locus_tag="ACHKUI_004979"
     CDS             1741..3888
                     /gene="mobQ"
                     /locus_tag="ACHKUI_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO6600985.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTR
                     KNGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAAR
                     ETVLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTH
                     AETWRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETE
                     RGEQNRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEAL
                     PTDSNAAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWVAPEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGL
                     ADLREQLRKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQH
                     VYSSATFEQLLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVI
                     NALTDKGLRFAGYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLF
                     RSLGFERTWDEWKRDEYARTHATERRHDRGWSQ"
BASE COUNT         1083 a         1026 c         1001 g          981 t
ORIGIN      
        1 aaaaaaaacg cgtcagagag cgttttagag gccttaaaat cgattctaga tcgtcatctt
       61 gatctgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc ccttcttgat
      121 cgtctcaagc gttacagcca agtcgctttt ttgattcagt tcgtcaacgc aaggtcttaa
      181 taattgctgg ttcagagact tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agggcgataa tacgttcccc tgtgctgcgg aattgctgga gaagttcgta
      301 tattcttatg ctgtaacttc ttgataaatt ggagatgttc ttaattacaa ctctagtaaa
      361 ttgccccttc aactgtgtca gataaggcat gaccgcatct gaaaaagtga tttgggcttt
      421 gccctcacct ttagcgtact gtgcccgata ctggatccag cggaactcct cgcgcttctc
      481 atcgtcctta aggatgatcg acctgtccca caaacgatca attgcttcct gtagctgaac
      541 ctcggcattt ttccgcccca tatcagggaa tgagtcgaaa tactcttcgg cggtaacagt
      601 catggtcttt tgcaattcag tggattctgc atcagaaccc gacttcaatc gaccaatgca
      661 aagtaacaag aatcgctgtt cctgaagagt caatttatag ctggcctcaa ccagtgcatt
      721 ggatttgtag gcaatcagat ccgacattgt aaatccctca acctaaggta atgagtaaaa
      781 tataaacatt cctttaggca aaggcaatat gatttaattt taatatccct tttcctgtga
      841 atattacctt aaatcatatt gcctttacct taaatcatat tgcctattca ccttaaatca
      901 tattgccttt accttaaatt atattaccta tagtcttcta aactcttgaa tttcataaga
      961 aaattcgacc ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acggcgagcc tgtggaaaac ttggggtgtt caaaacaaaa aatcatgaaa aacaacaggt
     1081 tacaaaaaag caggttcgca agctttagca aaaccccgct caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctgt cgaaaaaccg tgcgtatgtg ctcaatctgg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctggggctg
     1261 cggcgagcgg acggtgcatt cacgcctgtt ggtcgttttc tgacacttct ttggggactg
     1321 gttttggtaa cgcgttcagt tcgtcaatca acggctgtat cctgctcaca tcagcctgcc
     1381 gtgttacctc tgtcttcagg agatgaagca accaggctcg tagttgtgtt tccccaggct
     1441 tcctgacctt tgccagcaat gccgctccca caatgacctt ctgcgctgtg tccaggctcc
     1501 tgctcttcgc cttcagacgc tgcaatctgg cctcagcatc ggcaatcttc tgttcgagtg
     1561 ttctactcat tccgtaactc cgtgcgcggt gaaaaatcgc attttagcgc gtcactggta
     1621 gtttaaaaac tgaactggca taatgcacag caaatcacga agtgcgcact tatacaaact
     1681 ctccttcgtc gagtttgtgt gcgccctgcg gggctaaaag aaaacggcaa caacgacacg
     1741 atggcaattt atcatctcag catgaagatt ctttccagaa gcaagggcta cagcgccgtt
     1801 gcttctgctg cctatcgtgc cggagaaaaa atactggatg agcgcaccgg agtgattcac
     1861 gactacaccc gcaaaaacgg tgtagccagc gcagtgattc tgacgccagc aaacgcccca
     1921 gcatggtgtg cgaaccgcgc cgaactctgg aatgctgttg aaaaagccga gcggcggaaa
     1981 aactcacaac ttgcgcggga gttcgaactc gccatccccc gtgaactggc gcaggatgcc
     2041 gcacgggaaa cagttctcaa cttcgtaagg gaaaactttg tcagtcgcgg catgatcgcc
     2101 gacgtggcct ttcacaatct gggcgggtca aacccacacg cgcacatcat gctatccatg
     2161 agggcaatca cgcctgacgg cttcggggag aaagtccgtg agtggaatga ctggactcac
     2221 gccgaaacat ggcggggatc atgggcagac catgcgaaca gggcgctggc gaacgccggt
     2281 tatcaggaag agatagacca tcgttcatac gagcgacagg gggttgagaa aacgcctggc
     2341 attcacctcg gaaagtcggc ctgtgcgatg gaaaagtggg gcatcgaaac agaacgcggc
     2401 gaacagaatc gcctgattaa caggcttaac ctggaaatac aggtttcccg cgacgaactc
     2461 aggaacagtg agctgacaga gcccgcacgc cgtatggctg accttctgaa tattgagctt
     2521 cctgacaacg caacttcgta catactgcat gacatcatcg aagcgttgcc gacggacagt
     2581 aacgcagcat ggaaactcac cagtaaagcc atgtcgatga tggcagatat gcaggcgacg
     2641 cgcagacgct gggaagatct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
     2701 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggtt
     2761 gcaccggaat acggccgcga acaggaaaaa atccagtcgt tgagccagcg catggaaaaa
     2821 ttgcggcagc atcactggaa ggttgagaaa caggacattc ccgcgagtca aaaagcattt
     2881 gaaacacagt ggcagcagtg gggtgcatct ggccttgcag atctccgaga acagttgcgt
     2941 aaacgtgaag aggaattgcg ccggaaggag caggaggaaa cagaacgtcg tcgggcggag
     3001 cagctcagga gaaatgacaa tgccgttatg gattcagggg tactgaaagc agtcgttacg
     3061 gggtatggcg aggcccccat gccaggtaac ggggaaatga cctgttacct tctgcttcac
     3121 aaccggaacg gagaatatac gctctggggg aacgaactgg agaaataccg cgccagaatt
     3181 tttgagagtg ttgatttaat gcgtgacagg agtggttata tctgtgagcg aagcgaaatc
     3241 ggtcaacgtc caccactgca acatgtttat tcctctgcga cctttgagca actgctgacg
     3301 caggtttgcc agacatggcc gcaacatacc cgcgatctcc gccagccgaa gacatggccg
     3361 gagtctttct gtcttggaga agaccgccag ccagccatgc catcactggc ggcaaggaag
     3421 gtggatttta cgcagggacg tctgccaccg actctgatgc cggtaatgag ttctgtcgac
     3481 cgtgaaaccc gccagcttca gcttttgctg gtcatgggtg tggatgactc tatgggcggt
     3541 gttgtccggc ttaatggtac gctgtatccc gcgtttgccg tccccacagc cgacaacagc
     3601 cagctggtaa tcaatgcact gaccgacaaa ggtttacgct ttgcaggcta tggtgaagcg
     3661 gtaaaccacg atgctgacag tccggcacgt cctgcgccgg aacttatgca gtttcatctg
     3721 aaaacctgga gcgaaccgct ttttgctatc gtcaacacgc cagaaaaaca gccggatcat
     3781 ctgttccgct cgctgggctt tgagcgcacc tgggatgaat ggaaacgtga tgaatatgcc
     3841 cgaacacacg ccaccgagag gagacatgat cgcggttggt cacagtgaat gtgaccaata
     3901 ttttgttttg tttaacatcg tgttatgttc gtcgatgttc atcgacaggg atgaatgcaa
     3961 gaagcacaca cataacacaa atcaacttta tagttttccc gcccccgaaa aattgggggt
     4021 tcttttttca ctgtgccaga gcgtttatga cgcaattttt tacttcacca gtaccttcgc
     4081 atgccaaaca c
//
LOCUS       DAZFRE010000204         4068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFRE010000204 DAZFRE010000000
VERSION     DAZFRE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.209"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(131..721)
                     /locus_tag="ACHKUI_004980"
     CDS             complement(131..721)
                     /locus_tag="ACHKUI_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO6600986.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAVQQSPEHRS"
     gene            complement(759..968)
                     /gene="hha"
                     /locus_tag="ACHKUI_004981"
     CDS             complement(759..968)
                     /gene="hha"
                     /locus_tag="ACHKUI_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6600987.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1014..1475)
                     /locus_tag="ACHKUI_004982"
     CDS             complement(1014..1475)
                     /locus_tag="ACHKUI_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6600988.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1720..1932)
                     /locus_tag="ACHKUI_004983"
     CDS             complement(1720..1932)
                     /locus_tag="ACHKUI_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6600989.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(2061..2621)
                     /locus_tag="ACHKUI_004984"
     CDS             complement(2061..2621)
                     /locus_tag="ACHKUI_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6600990.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(2724..3584)
                     /locus_tag="ACHKUI_004985"
     CDS             complement(2724..3584)
                     /locus_tag="ACHKUI_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6600991.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(3643..>4068)
                     /gene="traX"
                     /locus_tag="ACHKUI_004986"
     CDS             complement(3643..>4068)
                     /gene="traX"
                     /locus_tag="ACHKUI_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO6600992.1"
                     /translation="GFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPL
                     SGTSYGIAGLLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMT
                     VLTVGLVSCAGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
BASE COUNT          996 a         1121 c          848 g         1103 t
ORIGIN      
        1 cggggattac tgtcgtttaa tgtgatttaa actgtgaaat agtatggttt tcagttatgg
       61 aaacgccggg agcggggaaa acttgctttt tcccgtttcc ggggttggac aactgagcaa
      121 cgcgaaggcg tcagctacga tgttccggtg actgctgaac agcccctgtt gtattcagcc
      181 attctccggt catctgcagc agttttctgg catcgtggct tctgagcaac ggaataccag
      241 cctgcctcag aacctcttcc agaagaatat cccggcgtct gcgttccggt cgtaaatggc
      301 tggcatcatc cagctctact gctgcaacaa tactgaacga acgccgctca acgataacca
      361 catcaacatg ccactgagac accatcctga ataactgcca ccactggcgt gatcgtggcc
      421 ggatattccc gttcagctgg acgatatccg cgacccgtac ctgcggacac agataccacc
      481 gcttcatatc cacaattctg aacagtcctt tcataaaact gacttccctt tctgtcagca
      541 gcgtcttctg acgttgccag tattcaccgc cggacagtat tcgctctgca tcaccggtcc
      601 gtatacctgc cgcatgcaat tgcatcagca aaggagaatg ctgatttaca cttctgagga
      661 taatccggat aagtaacgct gcaataatca gaacaataag aataaaaata atcagcttca
      721 ttgtttatct gctcctggta attaattatg ctgaattatt accttacata tttccatatc
      781 tcttttggta tcctgtcata taactttccg gtgattaatt ccgctaaccg gtggtctgct
      841 gctgaattaa atgattcacg ttcactgttt aataaactgt ctctgttttt atgaataatt
      901 ttttccagcg tattgactga tgaacatcgc cgtaactgat acagccattc ctgttttgtt
      961 ttcgccattt ttattcccgc tgagaacaga tgaatgataa gattgccatc gctttattta
     1021 cggtgtctcc atatccaggg gggaaccgga tcagcatctg accagagccc acgtttttgt
     1081 tgtctggctt cgttctgaag gacaggcagc acgggatctg tgttgtactg ctcatatacc
     1141 caggcagccc cggcccggac cataaactga ttaacattca ttccgtctgg tgcataaacc
     1201 tgcccgagaa tacgaccata acgatctcgc tgaaaatatg tgacggtaac cgtttttcct
     1261 gcaaccagtg atttcatcat gtccgttgac caccggccat aatcctgttt cttttccggt
     1321 gcatcaatat ttatcagcct gatccgcact gccttccgtg agtccatgac ctcgatggta
     1381 tcaccgtcca gtacacgaac aacccgtcca tgaatatctg cacacaatac cggccatgaa
     1441 aagataaata ataccagtgg aatatatttc ctcatgttat tctccatatc agataaatgg
     1501 cacctgtatt tcaggtaatc ctgaatatcg cagcacacac tgaataacac cttattttgt
     1561 gaaattaatc acaaaatata aaatcacgac ttaatatgga aaaaactctg tgctactcag
     1621 aactgtccgg tcatactttc cgaccggata ttaaaaaaag caaatctcat gcattattca
     1681 gccttctgaa tccctggagt aatgaaggct gctttttcgt tacacccttc cccggcagcg
     1741 ccggtggcag tgttcagccc tttttcgggc aaactgctgc cagtctgtcc ggggagcaga
     1801 ctgcgcagcc cgtagccatg cctcagcggc tcccggccag tcccccctga cttccagccg
     1861 gacagcccgg cgtccgacct gccggtagcg ccataccccg gagttttgcg aatcacgccc
     1921 tgttccgttc attttccttc cccctttaat ccaggccggt gaatgtgtta taaattttta
     1981 taaccttctt ctgccgggta tggaagaagg tttcagagaa gccttatttt tccgcctcct
     2041 ttagtgtgaa ggaggctttt ttattgttca tcaagcacgg cctgaagttc tgcctttatc
     2101 cggttctggc ggcggatttt atccagacgc tctgccgcat acacttcttc ctcctgagaa
     2161 atatgctccg tcacataccc ttccgtgtca taccggcagg caccggcttt catggcacac
     2221 agatagcttt ctgaacgggt gatggccttc agcgccctgc gcagtttttt atgcgagagc
     2281 gggatattcc gctgcgccac gtcttccagt aacacgtccc ggataccgca ggccagaagt
     2341 cggggtgtgt caccgtcaaa taatcccggc caccagggtt tcagggtgtt cacggcctca
     2401 tccagcgagg gcaggttcag ataaatggac agcgcctgtc tggcctgcgc tttttttgct
     2461 gtcagctctg cttcccgggc agccttctcc gccagtttct gctttttcac cttccatttt
     2521 ggtggcgtgg tgacattgat gatggttttc cggctccggg tcggtgtttc cccttccgtt
     2581 ttccgtttca gtgtcagtac cggtcgtttc tgctctgtca tgattgccct gacctttgtg
     2641 aaaatatgtc ctccaggtcg ttgaaactac tcactatgat attaagctga cctcaacgac
     2701 ctctgacata ttaataccga caattaaaga tgttttttaa accattgcgt ctgtactgcc
     2761 gccacacgtt caaaaaacgc gccttcataa atatcataat ggcatgcgtc agcttcctcg
     2821 tataattcct tagtaccaga agcaacagca tcatataagg cttttccctg ttctggtgga
     2881 ttaacactat cctggccggc aataacaatc aaaactgggc actgtacttt ggctgcgctc
     2941 tctgccggtt tatattgcaa cgtctccata accgtaagga aaggtatttt tatatccatt
     3001 tcaggatact gaccttttac cttctcgaag aatacctttg attcattatc actcagtacc
     3061 cgggtaaccc ccacaaacat ctctttcccg gtgtttttct tcttctctgc cattttattc
     3121 agcgtggaca gaaaagacgc tctctccgat tcattcatct ctccggtaac cagaacatca
     3181 ccgtcagcaa aagccagctg gctgacaata cattttacgc gttggtcctg cgcagctgca
     3241 ctgaagacat gcccccctcc cagcgatgtt ccccataacc caatacgctg attatcaata
     3301 cattcctgct tttctgccca attgataact gaaataatat cttcggtctg cattgcagga
     3361 accagacgcc ctcgttctcc atcactctca ccgaatcctc gataatcaaa agtaatcgtt
     3421 gcgaatcctg cttctgtgaa ggcattcgca aagcaaggga gcagaacatt cctgataccg
     3481 caaaacccat ggcataagat aatcaaagga tgttttatat ttccttcagg aacacgaaat
     3541 gtcagggcaa taccttcaga gagtttgtga tccgtaattt tcataaatat cctgtgttat
     3601 gttgtttatt aataatcaga atcagcgtca gtatcatacc agtcacaacg ccagaacgcc
     3661 cagcacagcc agatgacagg cgtagaatac cgggaaaaaa tccccgggcc agaaacgggg
     3721 taatgatttt cctgcacacg acaccagacc aacggtcagc accgtcatta caagaccggc
     3781 caccgccgct gcatcactgg aggcaaggtt aagtgccgga ataacggcca gcaggcatgc
     3841 aagcagcgcc agacgttccg ctctgtcttc cgcccggtac agtcggtaac tgaccgccag
     3901 catcagcagc ccggcaatgc cgtaactggt gccggacaaa ggcccccaca gggccatcag
     3961 aagaatggcc gctgctgtac gccacccact gcgcgtttca caccacgtca gcacctgtgc
     4021 cgccactgca aaggcaaaca ggatattccc ctcataccag ggaaagcc
//
LOCUS       DAZFRE010000205         4059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFRE010000205 DAZFRE010000000
VERSION     DAZFRE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.210"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            542..1759
                     /locus_tag="ACHKUI_004987"
     CDS             542..1759
                     /locus_tag="ACHKUI_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600993.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFIFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            1771..2889
                     /gene="nanY"
                     /locus_tag="ACHKUI_004988"
                     /gene_synonym="yjhC"
     CDS             1771..2889
                     /gene="nanY"
                     /locus_tag="ACHKUI_004988"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFO6600994.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            2932..3057
                     /gene="ythA"
                     /locus_tag="ACHKUI_004989"
     CDS             2932..3057
                     /gene="ythA"
                     /locus_tag="ACHKUI_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFO6600995.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(3110..3367)
                     /locus_tag="ACHKUI_004990"
     CDS             complement(3110..3367)
                     /locus_tag="ACHKUI_004990"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFO6600996.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            3390..3593
                     /locus_tag="ACHKUI_004991"
     CDS             3390..3593
                     /locus_tag="ACHKUI_004991"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6600997.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            3681..4059
                     /locus_tag="ACHKUI_004992"
                     /pseudo
     CDS             3681..4059
                     /locus_tag="ACHKUI_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1217 a          710 c          884 g         1248 t
ORIGIN      
        1 tcttggttct ggtgtttgta acaaactatc agctacaaaa atatgctcaa tttgtgacat
       61 cagtaacaaa acgcgttttg ttatgtggat tgcttgtttt ttgatctggt ataacaggta
      121 taaaggtata cagaaaagca aagaaatact gcaaaggaaa acagctataa cgtaagctaa
      181 agtaataacc tctcagtctt tcctcatttg acgaagggag ttttattcaa cctgaacgga
      241 ctacgaaaat gagcacaatg aataagtcaa tttttgaagc agggttgaac tgtgccagtg
      301 gtggaatatc tggcgaacat tgttttttgt tggtgaccca aaactgtagg tgggtatcag
      361 ttttatcttt catagagtga aatatgttaa gaagaaatgg aggaaaagat tgtactgatt
      421 aggtattgat aacaatcaat agtactggcg tatttgaaga caatataatt atttctggat
      481 attgttgagg ctccctaata tttactttaa gggctatatt agaataacac aggaaacaaa
      541 tatggcaaca gcatggtata aacaagttaa tccaccacaa cggaaagctc ttttttccgc
      601 atggcttgga tatgtatttg atggctttga ttttatgatg atattttaca ttcttcatat
      661 tataaaagca gatcttggca ttacggatat tcaggctact ttaataggga cagtggcctt
      721 catagccaga cctattggag gtggtttttt tggtgccatg gctgataaat atggtcgtaa
      781 gccaatgatg atgtgggcaa ttttcattta ctcagtcgga acaggcctta gcggtattgc
      841 tacaaactta tatatgctcg cagtttgccg ttttattgtt ggcttaggga tgtctggtga
      901 atatgcatgt gcttcaactt atgcggtaga aagttggcct aaaaatcttc aatctaaagc
      961 tagtgctttt ttggtaagtg gtttttctgt tggaaatatt attgcggcac aaataatccc
     1021 tcagtttgct gaagtatatg gatggagaaa ctcttttttt ataggcctgt taccagtttt
     1081 actagttctt tggatcagaa aaagtgctcc agaaagtcag gagtggattg aagataaata
     1141 taaggataaa tcaacatttt tgtctgtctt cagaaaacca catctttcaa tctctatgat
     1201 cgttttcctc gtctgttttt gtctatttgg tgcaaactgg ccgataaacg gactacttcc
     1261 ttcctacctg gcagataatg gagttaatac agtggtcatt tcaactctga tgacaatagc
     1321 aggtttagga acactgacag gtacaatatt ttttggtttt gttggtgata agattggtgt
     1381 aaaaaaagcc tttgtagtcg gtctaataac ttcatttatt ttcctttgtc ctcttttttt
     1441 tatttctgtg aaaaactctt ctcttatagg attatgtctc tttggattaa tgtttacaaa
     1501 tttaggtatt gcagggttgg ttccaaaatt tatatatgat tactttccaa caaaattaag
     1561 aggattaggg accggtctta tttataactt aggggcaact ggaggaatgg ccgcacctgt
     1621 attagctaca tacatttcag gatattatgg cttaggtgtt tcattattca ttgttacggt
     1681 tgcattctct gccttattaa ttttgttagt tggttttgat attccaggta aaatttataa
     1741 actatccgtg gctaaatgat tggaggcttt atgattaatt atggcgttgt tggtgttgga
     1801 tactttggcg ctgaattagc tcgttttatg aatatgcatg ataatgcaaa aattacatgt
     1861 gtatacgatc ctgaaaatgg agaaaatatt gcccgtgaat tgcagtgtat caatatgtca
     1921 agcttggatg ctttagtctc aagtaaatta gtcgattgcg tgatcgtagc caccccaaat
     1981 tatctgcata aagaaccagt aattaaagca gcaaagaata agaagcatgt tttttgtgaa
     2041 aaaccaattg cattaagtta tgaagattgt gtggatatgg tcaaagcgtg taaagaagct
     2101 ggtgtgacct ttatggccgg gcatattatg aattttttca atggggttca atatgcacgg
     2161 aagttaatta aagaaggtgt tatcggcgaa atattatcat gtcatactaa gagaaatggc
     2221 tgggaaaaca aacaagagag actttcctgg aaaaagatga aagaacaatc tggtggacat
     2281 ctatatcatc atatacatga gttagattgt gttcagcatt tacttggaga aataccagag
     2341 acggttacta tgattggtgg aaatttggcc cattctggtc caggatttgg caatgaagat
     2401 gatatgttat ttatgacctt ggaattcccg tcaggaaaac tagcaacctt agagtggggg
     2461 agtgcattta actggccgga acattatgtc atcatcaatg gaactaaagg ctctattaaa
     2521 attgatatgc aagaaacagc agggtcactt aggattggcg gtcagacaaa gcattttttg
     2581 gtccatgaaa cacaagaaga agatgatgat cgtcggaaag gcaatatgac ctcagaaatg
     2641 gatggcgcta tagcatatgg tcatccagga aaaaaaacac cattatggct tgccagttta
     2701 ataagaaagg agacgttatt cctccataat atcctctgtg gtgcaaaacc tgaagaagat
     2761 tatattgacc ttctcaatgg tgaggcggcc atgtcggcga ttgctactgc tgatgctgcc
     2821 actctttcaa gatcgcagga caggaaagtg aaaatcagtg agatcattaa acatacatca
     2881 gtaatgtaaa aactatacgc ctcttgattt aattcaggag gccttttatg tatgatcaag
     2941 aattttatct tcgataatct cataatttta gcagtgccat ttatgataaa aacatccctt
     3001 aaaaccaatc taatcttttt cttcctctgt gtatttgttc cccatatggc gtcataattt
     3061 acgctacgta atacgggata gttacgatac gcagcgatag cgctaagttt tagttaaaat
     3121 cccccatcag cgggatatgg ggatctctgg gaggttcagg ttttagcatc gcgactttaa
     3181 tacgacagac gctggtgacg atgatgccaa gtagtgactc ttccgaataa ggttcccact
     3241 gaccaattgg cgcgcgcttc gcaataaaat ctcccttcgc ccagtattgg gaaaagtaga
     3301 tacattcaaa ctgtgtacgc tgtttcgtct cacagttcac gacattgctg gccgatgagc
     3361 tgaccattgg gcctatccgg gctgtcccga tggatattac gccgaagtat gtgggaattg
     3421 ccagcggatt gatgaacgcc ggttccgctg tcgccgacat tatttcacct atcgcctttg
     3481 gcattattat cgacaaaaca ggcaactgga gtttaccatt ctacggttcc gtcgcattgc
     3541 ttgttattgg tatattcctg acgttcttca tgcgtccaga taagtcattg taaaagtcga
     3601 atcagggctg aagtggcaca ctgaatttgg ccacctgaac agaggtgata tgctcacctc
     3661 agaacaacac aggtgctcca atgaaaaaaa gaaatttcag cgcagagttt aaacgcgaat
     3721 ccgctcaact ggttgttgac cagaaataca cggtggcaga tgccgcgaaa gctatggatg
     3781 ttggcctttc cacaatgaca agatgggtca aacaactgcg tgatgagcgt cagggcaaaa
     3841 caccaaaagc ctctccgata acaccagaac aaatcgaaat acgtaagctg aggaaaaagc
     3901 tacaacgcat tgaaatggag aatgaaatat taaaaaggct accgcgctct tgatgtcaga
     3961 ctccctgaac agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc
     4021 tgccatgtgt tcggggttca tcgcagcagc tacagatac
//
LOCUS       DAZFRE010000206         4057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFRE010000206 DAZFRE010000000
VERSION     DAZFRE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.211"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1616)
                     /locus_tag="ACHKUI_004993"
     CDS             complement(<1..1616)
                     /locus_tag="ACHKUI_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6600998.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQVSQRELMAISKRTGTAFSDNASLFARSAASMREYGYSSEDVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNAVNESGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVS
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQALLTRNIAART
                     AAQTTLNNVTSVGSRLLSGALGLVGGVPGLVMLGATAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALKEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLA"
     gene            complement(1665..2006)
                     /locus_tag="ACHKUI_004994"
     CDS             complement(1665..2006)
                     /locus_tag="ACHKUI_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6600999.1"
                     /translation="MEFLLISIVLGLIPAIIAKSKGRSFLGWWVYGAMIFIVALVHSL
                     VIQRDEKAHEQQMVSNGMKKCPYCAELIKEEAIKCKHCGSDLICNADSSVSQKTDDEY
                     LQEARRKAGLL"
     gene            complement(2064..2342)
                     /locus_tag="ACHKUI_004995"
     CDS             complement(2064..2342)
                     /locus_tag="ACHKUI_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6601000.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRISPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQAEGVSDPFAEIEKALFVAAR"
     gene            complement(2366..2737)
                     /locus_tag="ACHKUI_004996"
     CDS             complement(2366..2737)
                     /locus_tag="ACHKUI_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6601001.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVSEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(2752..3456)
                     /locus_tag="ACHKUI_004997"
     CDS             complement(2752..3456)
                     /locus_tag="ACHKUI_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601002.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTEPLKVKVDLNSTLQVA
                     SGSKLEMVVEAAGGVPPYSYVWKKGSSPVSGQTAATFSKVSAASGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(3517..3861)
                     /locus_tag="ACHKUI_004998"
     CDS             complement(3517..3861)
                     /locus_tag="ACHKUI_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6601003.1"
                     /translation="MREATLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADDIREQALDAVRQLVPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(3858..>4057)
                     /locus_tag="ACHKUI_004999"
     CDS             complement(3858..>4057)
                     /locus_tag="ACHKUI_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6601004.1"
                     /translation="RTGNSDNSMKASDPRNAFYWRFVELGTINMPAHPFIRPAFDTTE
                     ELAAQVAIQRMNQAIDEVLSK"
BASE COUNT         1002 a         1164 c          889 g         1002 t
ORIGIN      
        1 gccagttgat ttgttgcttc tgcaagccct tctgtgacag tttttaccga cgtcatgtgg
       61 ttaatcataa aaccgttatc ggttgtccag cccgggtttg ccagcacata ctgatagcca
      121 gcaatttttt cctgtaagga tttaatctta cttttctgct cgtcaattaa cctgttctgc
      181 tccttcagtg cctgtcgcgt cttttcctca ttatctgacg cttcaggaag cgacattgcc
      241 gacgttttct ggcgaatttc gtcgattgtt gcggcatact ggcgtgcaga ttctctggcc
      301 tgctcctgat tctgatacat cgtgtaccag gccgtcgccc ccagcataac gagtcctggc
      361 acaccaccaa ccagccccag cgcaccactt aacagacggc tccccactga cgtaacatta
      421 ttcagcgttg tctgtgccgc tgttctggcc gcaatattac gggtaagtaa cgcctgggca
      481 gctgtcagct tcgcttctgc tgcggcctgc ctttcggtac cgcgagcagc aacaaccgcc
      541 tgttgcgcac gataaaccgc cgcacgcgcc ctggcggttg ctatctgtgt cccccgaagt
      601 tgcgcttcag caagagacac ttcgtttctg gccgcggtga ttagcccggc agttgcagat
      661 ccagcagacg acgccatatt gccaaaatat cgggctaccc cgacggcaac cagagcaccg
      721 gcagcggttg ccacggtatc aatattgcct gcaataccat tcagcacacc agaaagcgtc
      781 ttcgtcactc cgcttgcctc gttcgcacca ccaacccagg ccataaaggc gttttcaact
      841 ttggttgcag aggatgaaac cgtatcaggc attgccgcat attcatcacg taacgcccca
      901 agctgactaa tcagtgcagg aacaacctta tcggcggtca actttccgtt atccgccatg
      961 gccttcagat ccttacgggc aacacccatt cccgcagcca gcgcacgaat aacacgatca
     1021 ccgctctcat tcacggcgtt aaactcttca ccgcgcagca ctccctgcgc cagtgcctga
     1081 ctgaactgcg tgatcaccga actggcttct gctgtactgg caccggataa cttcaggccc
     1141 gtggagatcg cctcggtgac tttcagtaca tcctcagaac tgtagccata ctcccgcatg
     1201 gaagctgcag agcgggcaaa aaggctggcg ttatcagaaa aagccgtccc cgttctcttg
     1261 ctgattgcca ttaattcgcg ttgtgatacc tgaaaatcat cactggactg tgaggcctgc
     1321 ttcaggcggg catttacgga attccactca tcggcgagag aaataagatg accagtagca
     1381 aaagccccgg caaatgcccc cgccatattc agtgccgaag atttagctgt atttatctga
     1441 tccgtcactt ctgccagtgc acgccgcatt tcacgggatg cagcagcgga ctgtcggcct
     1501 ccgttctgca tggtgcggta gtaatcctgc cccatacgcg aagcccggga gatctctgac
     1561 tggaacgacc gggagtttgc cgagatttta ataatcagtt cacgtaatgt tgccactctc
     1621 attctccgga cgaaaaaaaa ccgccgaagc ggtttttgta ttctttaaag aagtccggcc
     1681 ttacgccttg cttcctgaag atattcatca tccgtttttt gtgaaacgga actatctgca
     1741 ttgcaaatta aatcactacc gcaatgctta catttaatag cctcttcttt gatcaactcc
     1801 gcacagtaag ggcatttctt catgccattg cttaccattt gttgttcatg tgccttctca
     1861 tctctttgga ttacaagcga atgaaccaga gcaacaataa agatcatcgc tccatatacc
     1921 caccacccta aaaatgagcg acctttgctt ttcgcaataa tagctggaat tagtcccaaa
     1981 actattgaaa taagtaaaaa ttccatcagt gtttccttac tttctcaata aaatgggaat
     2041 aataatccag tcaagtgaaa agatcatcga gctgcaacaa ataatgcctt ctcaatctca
     2101 gcaaatggat ctgagacgcc ttctgcctgc tctttttccc actgaagaag cgcatcattc
     2161 agtggtactt tgacaccctg cgcaccgtaa acagctgaaa ctatctgggc agcccggata
     2221 tcagcccgct cgtcacccag cgggctgatt ctgtcaaatt ctgcccacat catgatttct
     2281 gatgcggaca tttcccggcg taactctgac aatgtgcgcc ccatcctgag cgccagcatc
     2341 atcagaaaac gcatccccgg aagcgctact tttttttaac ctcgccggca tcactgatca
     2401 gttccagaga ctgccgaaga agccgcgcat gcaccgggcc atacacggca atcacctgtt
     2461 cacgatcatc ctctgaaaat acaggttgca gtccggtatc acacagaaca tcaatgaaca
     2521 gttcaacatc tgcctccaga ttccggcggg cgcgctccgc aacagataac ggtgtctcat
     2581 catcttttgc tttaacgatc tcctgccagc gcaaccaggc ttctgcagaa ggttcccgta
     2641 atacaaccgt tgccccttcc cattcagaca catcaacagt tttatggcga aaccccgaca
     2701 tcgttgccag tgccagatta cggatatttt tagtcatcac atctatcctc attaactgac
     2761 ggtaacagtg caggaagtgg aggtcacttt gttaaccggg cttgctgaat cagaaatctc
     2821 gcaggtatac gcaccggcat caccggatgc tgctgatacc ttactgaacg ttgccgccgt
     2881 ctgtccggaa acaggagaac tacctttctt ccagacataa gaataaggcg gcacaccacc
     2941 cgcagcctca accaccattt caagtttcga accggaagca acctgcaacg tgctgtttaa
     3001 atcgaccttc actttcagcg gctctgtcgt caacaccggt ttacctttca ggcgcaggga
     3061 aaacgttgca gccacaacac cattggttcc tgcagaccag gtatgctgac gcacctctgc
     3121 cataaaggta aatccgtttc ctgacggaaa aataacttta aagccatacg tggtgtcatt
     3181 gtcataggca ctgcgcaacg cgttctgggc agcattgagg taaaagttgc ctgacatgga
     3241 aatctctgac gcggcaccaa gaccgttaat attttcctgc tcaacagaac acagcgtggt
     3301 gacatcaata tcctgctttt gtcctgcggt aaactgcacc tctttgattg tacagctcag
     3361 gccaagatag ctggcagaat ccagggtttc tgctgttacc ggtgcagacg aaatcataat
     3421 tttcgtcagt tgcgaacgct caaaattaga ggacatactc gtctcctgaa aataaaaaaa
     3481 accgccagcg gcgggtgggt aaaatcatta acgacctcag gctattacct gaaattcaag
     3541 cgtggctctg ctcagacggg aatcaggatc ataaccctga gttttagaaa taacggaggg
     3601 tacaagttgc cttaccgcat caagcgcctg ttctcggata tcatctgcgt catcaggcac
     3661 cgttgcccag acatcgatct gcacggtaat tctggattca gcctgaccat caagcacatc
     3721 agacgccgtg tcagacacca cagaaaacac cagccacggc ggagataccg caggctttcc
     3781 ctccgtcagc gggaccacat agggataaac ctgtcctccg gccagttgag acagcaggga
     3841 atacagtgtg gcttccctca tttacttaag acctcatcaa tagcctgatt cattcgctgt
     3901 atggcaacct gcgccgccag ctcctctgtc gtatcgaaag ccgggcgaat gaatggatgc
     3961 gcgggcatgt ttatcgttcc cagctccaca aagcgccagt aaaacgcatt tcggggatcg
     4021 ctggctttca tgctgttatc actgtttccg gttcgca
//
LOCUS       DAZFRE010000207         4052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFRE010000207 DAZFRE010000000
VERSION     DAZFRE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.212"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1079
                     /locus_tag="ACHKUI_005000"
     CDS             <1..1079
                     /locus_tag="ACHKUI_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6601005.1"
                     /translation="QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDR
                     ILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLL
                     RTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWAD
                     IVAQGTRHSMKASSDNNDFRARGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLD
                     DGQDNAGYVKFGHGSTQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHRVSELPVNW
                     WVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQA
                     GYAHSVSGSSAEGYNGQATLNVTF"
     gene            1200..3716
                     /locus_tag="ACHKUI_005001"
     CDS             1200..3716
                     /locus_tag="ACHKUI_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601006.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISLMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWQTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNRPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGVLVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELPA"
     gene            3793..>4052
                     /locus_tag="ACHKUI_005002"
     CDS             3793..>4052
                     /locus_tag="ACHKUI_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6601007.1"
                     /translation="MIHLFKTCIITGVILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEA"
BASE COUNT          954 a         1063 c         1115 g          920 t
ORIGIN      
        1 tgcaggccgg ggcctttaac tacaccctca accgggacag tgatgagagc tggtatctgc
       61 gcagtgaaga acgttatcgt gctgaagtcc ccctgtacgc ctccatgctg acacaggcaa
      121 tggactatga ccggattctg gcaggctcac gcagccatca gaccggtgta aacggtgaaa
      181 ataacagcgt ccgtctcagc attcagggcg gccatctcgg tcacgataac aacggcggta
      241 ttgcccgtgg ggccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg
      301 acctgctcag aacagaggtc gccggtatgt cactgacgac aggagtgtat ggtgctgcag
      361 gccattcttc cgttgatgtt aaggatgatg acggctcccg cgccggcacg gtccgggatg
      421 atgccggcag cctgggcgga tatatgaacc tgacacacac ctcctccggc ctgtgggctg
      481 acattgtggc acagggaacc cgccacagca tgaaagcgtc atcggacaat aacgacttcc
      541 gcgcccgggg ccggggctgg ctgggctcac tggaaaccgg tctgcccttc agtatcactg
      601 acaacctgat gctggagcca caactgcagt acacctggca ggggctctcc ctggatgacg
      661 gccaggataa cgccggttat gtgaagttcg ggcatggcag tacacaacat gtgcgtgccg
      721 gcttccgtct gggcagccac aacgatatga cctttggcga aggcacctca tcccgtgaca
      781 ctctgcgcga cagtacaaaa caccgtgtga gtgaactgcc ggtgaactgg tgggtacagc
      841 cttctgttat ccgcactttc agctcccggg gtgacatgag catgggtaca gccgccgccg
      901 gcagtaacat gacgttctca ccgtcccgga atggcacgtc actggacctg caggccggac
      961 tggaagcccg tgtccgggaa aatatcaccc tgggcgttca ggccggttat gcccacagcg
     1021 tcagcggcag cagcgctgaa ggctataacg gtcaggccac gctgaatgtg accttctgac
     1081 agaaccatcg cctctttgtg gtcccggtca tcatgatcgg gacccggacc ggcgcaacgg
     1141 atattcaacg ccacattcgc tggcattaac aataacatga tattcatcac ggagtgacta
     1201 tgttacagat agtcggcgcg ctgatcctgc tgatagcagg atttgccatt cttcgccttt
     1261 tgttcagagc attaatcagc acagcttctg cgctggcagg gcttatattg ctgtgtctgt
     1321 tcggcccggc cttactggct ggctatatca ccgaacgcat aacccggttg ttccatattc
     1381 gctggctggc aggcgtattt ctgacgattg ccggaatgat catcagcctc atgtggggac
     1441 ttgatggtaa acatatcgcg ctggaggctc acacctttga ctctgtgaaa tttattctga
     1501 ccaccgctct cgccggtggt ctgctggctg ttcccctgca gatcaaaaac attcagcaga
     1561 acgggatcac accagaagat atcagcaagg aaattaacgg gtattactgc tgtttttata
     1621 ctgccttttt ccttatggcg tgttctgcat gcgcaccatt gatcgcgtta cagtacgata
     1681 tttcaccgtc actgatgtgg tggggcgggt tgttgtactg gctggctgca ttagtgacgc
     1741 tgctatgggc ggccagccaa atccaggcgc tgaaaaaact gacctgtgcc atcagccaga
     1801 cactggaaga acaaccggtg ctcaacagta aatcgtggca gaccagtttg caaaacgatt
     1861 acagccttcc tgactcactg acggagcgca tctggctgac gctcatttct caacggattt
     1921 cccggggaga gctgagggaa tttgaactgg cagacggaaa ctggttactg aacaatgcct
     1981 ggtatgaaag aaacatggca gggtttaacg aacagttgaa agagaacctg tcattcacac
     2041 ctgatgaact gaaaacgctc ttccggaacc gcctgaattt atcaccggaa gcgaatgacg
     2101 attttctcga tcgttgcctg gacggcggtg actggtaccc cttttcagaa ggccgccgtt
     2161 ttgtatcatt ccaccacgtg gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag
     2221 tacatcatgc cccggaaaat cataagccgg ctccggaatg gtactgctcc tctctttgtc
     2281 acgaaacaga aacactgtgt caggacattt atgaacgttc ttacaccggt tttatttccg
     2341 atgcaacggc gaatggtctg attctcatga aactgccgga aacctggagt acaaatgaga
     2401 aaatgtttgc ttccggaggg cagggacatg ggtttgccgc tgaacgggga aaccatattg
     2461 tcgacagagt ccgtctgaaa aacgcacgga tcctcggtga taataatgcc agaaatggag
     2521 cagacagact ggtcagcgga acagaaatcc agacgaaata ttgttcaact gcagcccgta
     2581 gcgtcggtgc ggcattcgac ggacaaaacg gacagtatcg ttacatggga aataatcgcc
     2641 ccatgcaact ggaagtcccc cgtgatcagt atgccggcgc tgttgaaacc atgaggaata
     2701 agatccgcga aggtaaagta cccggtgtaa ccgatcccgc agaagcgtca cggctgattc
     2761 gtcggggaca tctgacttat actcaggccc gtaatatcac ccggttcggg accatcgaat
     2821 cagtcactta tgatattgcc gagggttcgg ttgtcagtct ggcggccgga gggatcagtt
     2881 ttgccctgac ggcatcggtc ttctggctca gcaccggcga tcgcgatgct gccctgcaga
     2941 cagctgctgt ccaggcagga aaaaccttca cccgcacact ggctgtctac gtcacaaccc
     3001 agcaactcca ccggctcagt gttgttcagg gtatgctgaa gcacattgat ttttcgactg
     3061 ccagcccgac tgtccgactg gctcttcaga aggggaccgg tgcaggaaat atcagtgccc
     3121 tgaacaaagt gatgaagggt acgctggtga catctctggc actggtagct gtcacaaccg
     3181 gccctgacat gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc atcaggaatc
     3241 ttgccgtggc atcttccggt gtggcaggtg gagctgtcgg gtcagtggcg ggcgggatac
     3301 tgttcagtcc actaggacca tttggtgcac tgacagggcg tgtggttggc ggtgttctgg
     3361 ggggaatgat tgcctccgct gtatcaggaa aaattgccgg agtgctggtt gaagaagatc
     3421 gcgtcaaaat tctggcaatg attcaggagc aggtgacatg gcttgccggc agtttcctgc
     3481 tgaccggaca tgagattgaa aatctgaacg agaatctggc ccgtgttatc gatcagaatg
     3541 ctctggagat cattttcgcc gccggtatac aacaacgggc cgcgaccaat atgttaatca
     3601 aaccactggt ggtcagtatc atcaggcaac gccccgtcat ggaatatgat gcatcccatc
     3661 tcggcaatat ggttaaccga ctggaagaag cattcccccc ggagctcccg gcataacgtg
     3721 tgttctgctc cttttaggtt ttatcacctg atttgccagt gtcatcgtca tgactcatga
     3781 gaggtaaata ccatgattca cctgttcaaa acctgcatta ttactggcgt cattctgggg
     3841 ttaacgtggt ctgccccact ccgggcacag gatcaacgtt acatcagtat acgcaataca
     3901 gatacgatat ggctcccggg aaatatttgt gcttaccagt tccggctgga taatggcgga
     3961 aacggtgaag gatttggccc cctcaccatc actctgcaac tcaaagacaa atatggtcag
     4021 acgctggtga ccagaaaaat ggaaacggaa gc
//
LOCUS       DAZFRE010000208         4021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFRE010000208 DAZFRE010000000
VERSION     DAZFRE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.213"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..312
                     /locus_tag="ACHKUI_005003"
     CDS             <1..312
                     /locus_tag="ACHKUI_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFO6601008.1"
                     /translation="SSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYPQTVLDS
                     VLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGGST
                     L"
     gene            309..3131
                     /gene="traG"
                     /locus_tag="ACHKUI_005004"
     CDS             309..3131
                     /gene="traG"
                     /locus_tag="ACHKUI_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO6601009.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTRGSAGMY
                     GDAHAEWGVKPGILGVGGGVGVRGGGRASIDWSDEDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKTEALSQNNKYNEEKSG
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            3146..3649
                     /locus_tag="ACHKUI_005005"
     CDS             3146..3649
                     /locus_tag="ACHKUI_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO6601010.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            3681..>4021
                     /gene="traT"
                     /locus_tag="ACHKUI_005006"
     CDS             3681..>4021
                     /gene="traT"
                     /locus_tag="ACHKUI_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO6601011.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWL"
BASE COUNT         1053 a          884 c         1118 g          966 t
ORIGIN      
        1 tccagcagcg tggtttacca gctgacggac tatatcggct acgacatcat gctccagtac
       61 attcaggaac tgcttcagca ggcccgggcc atgattgcca ccgggaacta tccacagacc
      121 gtgctggaca gtgtgctgga gaatctgaat caggcacagc aacagattgc ggttttccag
      181 gggcaggtaa aggtccagca ggatgcattg ctggttgttg accgtcagat gagctatatg
      241 cgccagcagc tttccgcccg catgctcagc cgttaccaga acaactatca cttcggaggg
      301 agcacgctgt gaatgaagtc tatgtgattg ccggtggtga gtggttacgc aataacctga
      361 acgccattgc cgcctttatg gggacccgga cgtgggattc catcgaaaaa attgcgctca
      421 cattgtctgt tctcgcggtg gccgtgatgt gggtacagcg gcacaacgtg atggatttgc
      481 tgggctgggt ggccgtgttt gtgcttatca gcctgctggt taatgtccgt acatcggtgc
      541 agattattga taacagtgac ctggtccagg ttcaccgggt ggataatgtg cctgtcggac
      601 tggcgatgcc actttcactg acgacccgta tcgggcatgc aatggtggcc agttacgaga
      661 tgatattcac ccagcctgac agcgccacct acagcaagac aggcatgctg ttcggggcga
      721 acctgattgt gaaaagcacc gattttctgt cccggaatcc ggaaatcatc aatctgtttc
      781 aggactacgt ccagaactgc gtgctggggg atatttacct gaaccacaaa tacacgctgg
      841 aagacctgat ggcctccgct gacccctaca cgctgatttt ttcccggccc agcccgctac
      901 ggggcgttta tgacagcaat aataatttca taacctgtaa agatgcgtcg gtcacgctga
      961 aagacaggct gaatctcgat acaaagacgg gaggcaagac ctggcattat tatgtgcagc
     1021 agatatttgg cggcagaccg gacccggacc tgttattcag gcaactggtg agtgacagtt
     1081 acagttattt ctacggctcc agccagtctg ccagtcatat tatgcgccag aacgtcacca
     1141 tgaatgccct gaaagagggt atcaccagta atgcagcccg taacggtgat accgccagcc
     1201 tggtcagtct ggccacgact tcgtcgatgg agaaacagcg tctggcacat gtctccattg
     1261 gtcatgtgct gatgcgaaac ctgccgatgg tgcagaccat tctggtgggc attaccattg
     1321 gtatcttccc gctcctggta ctggcggctg tcttcaacag actgacgctg tctgttctga
     1381 gaggctatgt gtttgctctg atgtggttcc agacctggcc gttgctgtat gccattctga
     1441 acagtgccat gacattctat gcaaagcaga atggtgcacc ggtcgtgctc tctgaactct
     1501 ctcagataca gctgaaatac tccgacctgg cgtccactgc cggatatata tctgtcatga
     1561 tacccccgtt gtcatggatg atggtaaaag gccttggcgc aggtttttcc agcgtgtaca
     1621 gccatttcgc ttcctctgct atcagcccga ctgccagtgc agcaggcagt gtggttgacg
     1681 gtaattactc ctacggcaac atgcagacgg aaaacgtgaa cggcttcagc tggagcacca
     1741 acagcaccac gtcgtttggt cagatgatgt accagaccgg cagtggcgca actgccacac
     1801 agacccgtga cggtaatatg gtgatggatg caagcggagc gatgtcccgg ttaccggttg
     1861 gtatcaatgc aacgcgtcag attgcagcgg cacaacagga aatggcccgg gaggcgtcga
     1921 acagagcaga aagtgccctg catggtttca gcagcagtat tgccagtgcc tggaactcgc
     1981 tcagtcagtt tggttctaac cgggggagca gtgattctgt caccggtggt gctgacagta
     2041 cgatgagcgc acaggactcc atgatggcca gccgtatgcg cagtgcagtg gaaagctatg
     2101 cgaaggcgca taatatcagt aatgagcagg cgacaagaga actggcatca agaagtacga
     2161 gaggttctgc ggggatgtat ggcgatgctc atgctgaatg gggggttaaa cctggaattc
     2221 ttggtgttgg tggtggcgta ggtgttagag gtggtggtcg ggcaagtatt gactggagtg
     2281 atgaggatgc gcatcaggcc agcagtggtt cccgggccag ccatgatgct cgccatgata
     2341 tcgatgccag ggctacacaa gactttaaag aggccagcga ttactttacc agtcgcaagg
     2401 tcagtgaatc cggcagccat acggacaata atgccgattc ccgtgtggac cagctgtctg
     2461 cagccctgaa ctcagcaaaa cagagttacg accagtacac gacgaatatg actcgcagcc
     2521 atgaatatgc tgaaatggca tcccgtactg aaagcatgag tgggcagatg agtgaagacc
     2581 tgtcgcaaca gtttgcacag tatgtgatga aacacgcccc gcaggatgcc gaagctattc
     2641 tgacaaacac cagttcaccg gagattgcag aacgccgtcg ggctatggcg tggtcttttg
     2701 ttcaggaaca ggtgcagcct ggtgttgata atgcctggcg tgaatcccgt ggggacatcg
     2761 gtaaaggaat ggagagcgta ccttcgggtg gtggcagcca ggatattatt gctgatcatc
     2821 aggggcatca ggccattatt gagcaaagaa cgcaggacag taatatccgt aatgatgtaa
     2881 aacatcaggt tgataatatg gtcacagaat accgaggcaa tatcggagat actcagagca
     2941 gcatacgtgg cgaagaaaat attgtaggaa gacagtattc tgaactacaa aatcaccata
     3001 aaacagaggc attatctcag aataataaat ataatgaaga gaaatcgggt caggaaagaa
     3061 tgcccggggc tgacagtcca caggaactga tgaaaagagc aaaggagtat caggataagc
     3121 acaagcaata atattgtatg gggagatgat aatgaaaaat ttagctcata ttgctctggt
     3181 gacggtgata cagttcattg cctgttatct ggctgattgg gggagtgccg aaacagtttt
     3241 tatcctgtgc tttattgtac tatggcaggg attgtttata tggttatttt ctcaaatacg
     3301 aaaaaaacgg catgtgtcgg atgagtttaa attcagtaaa ggcgtttggt atatactgat
     3361 gccagtatct tccttactct ctccgttatt aagcctgatg gtttttatca ttggtacact
     3421 atacgaatta aggcgaatta gtggttgtgt aagtgtaaga gaatggatgc aatctcaggt
     3481 aaatgaacaa actaatgaag atttgcatct ggattttgat gatgtgaatt ttgattatgt
     3541 ggatttttat cgtactaatc cagcaacagg attagctatg catggaggat ttgattcagc
     3601 aggaaatact tttgggactt gttggcagga ctatgatgac aggcgataag aaaactgtgg
     3661 tttgtaaaaa aggtaatttg atgaaaacaa aaaaattgat gatggttgca ctggtcagtt
     3721 ccactctggc cctttcaggg tgtggtgcga tgagcacagc aatcaagaag cgtaaccttg
     3781 aggtgaagac tcagatgagt gagaccatct ggcttgaacc cgccagcgaa cgcacggtat
     3841 ttctgcagat caaaaacacg tctgataaag acatgagtgg gctgcagggc aaaattgctg
     3901 atgctgtgaa agcaaaagga tatcaggtgg tgacttctcc ggataaagcc tactactgga
     3961 ttcaggcgaa tgtgctgaag gccgataaga tggatctgcg ggagtctcag ggatggctga
     4021 a
//
LOCUS       DAZFRE010000209         4014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFRE010000209 DAZFRE010000000
VERSION     DAZFRE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.214"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..751)
                     /locus_tag="ACHKUI_005007"
     CDS             complement(<1..751)
                     /locus_tag="ACHKUI_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601012.1"
                     /translation="MSNDRGSSSGENQKTEQCIQEFQVTNQFKKMMKESLEEQKKVID
                     KRVKTLTHWSDEAEDEFRRIFGVPSEKIITIKFKLNGEVTKETKSARAVIQEAVDRMK
                     FICDKLSADKGECKEVTFIDKYLDSNDNYYDKDVTKWKCGNFVNSTDNDAYTANVTPD
                     HIIGVSPDKYVDVVTIRIGQRFVCKPMTGKDSKVSSLCHELTHLVRYGPKGMYGGMQS
                     EDMPVDKELQNAKEYDIFADKLIKNKDMTLFE"
     gene            complement(744..3260)
                     /locus_tag="ACHKUI_005008"
     CDS             complement(744..3260)
                     /locus_tag="ACHKUI_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6601013.1"
                     /translation="MNIPALNFDHSHHKLKIRGLQSPVDVLTFTGHEQLSSPFRYDIQ
                     FTSRDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARY
                     EVRLEPRMVLLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFFDDQSGYERGLTLPLRHPSGL
                     HDSATEAVWGLNTAYSVVEKSVTTRDYNYREATAEMTTGQHDATGGDKTTYGEAYHYA
                     DNFLQKGDKEVAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADTQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGV
                     AIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAQKSIVITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIESGEFNAKFLFTDDDGLPYANTKYIACFSDGTQ
                     KEGITDENGYTENFNTDSKQTIDVRLLNQNIDMILGGVHE"
     gene            complement(3517..>4014)
                     /locus_tag="ACHKUI_005009"
     CDS             complement(3517..>4014)
                     /locus_tag="ACHKUI_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601014.1"
                     /translation="SSMCGKTFAITTNNVLLDYLSSKCEFDEYNFDHDWIEIPARQSR
                     VFSFILNDAYVFLPGEHRYDIGSLEYYFTDVRWIRKKKIYRSLFDIMKFRVGICSSRL
                     GGDYLFQKDNGYCASEDEDSINIEYFLWRFGLNGGIEKMYFNIRTNQVVIDIDGSDVH
                     SLYDN"
BASE COUNT          909 a         1065 c          881 g         1159 t
ORIGIN      
        1 tttcaaagag tgtcatatcc ttatttttta ttaatttatc agcaaagatg tcatactctt
       61 tcgcattctg gagctcttta tcaacaggca tatcctctga ttgcataccg ccatacatac
      121 ctttggggcc atatctgact aaatgtgtta attcatggca aagtgatgaa actttagaat
      181 cctttcctgt cattggttta catacaaacc gctggcctat tcttattgtc actacatcta
      241 catatttgtc tggagaaaca cctataatat gatcaggggt gacgttagca gtataagcat
      301 cattgtccgt agaatttaca aaattgccac acttccactt agtaacatct ttatcatagt
      361 aattatcatt gctatcaaga tatttatcta taaaagtaac ttctttgcac tcccctttat
      421 cagcagataa tttatcacat ataaatttca ttctgtccac ggcttcttga ataactgctc
      481 gcgcactttt tgtttctttt gttacttctc cgttcagttt aaattttatg gttattattt
      541 tttcagatgg cacgccaaat attctcctga attcatcttc tgcttcatca ctccagtgcg
      601 ttaatgtttt taccctttta tcaattacct ttttttgttc ctctagagac tccttcatca
      661 ttttcttgaa ttgattcgta acttgaaatt cctgtatgca ttgttcagtt ttctgatttt
      721 caccacttga actacctcta tcattactca tgcacgcctc ccaatatcat gtctatattt
      781 tgattcaata atctgacatc aattgtttgt ttggaatcag tattaaaatt ttctgtatac
      841 ccattctcat cagtaatacc ttctttttga gtgccatcag aaaaacatgc aatatatttt
      901 gtgttggcat aaggtagacc atcatcatca gtaaaaagga acttagcatt aaattccccg
      961 ctctcaatca ctggaaacgc catcaactcc ggtgtcagct tcgccttcgg cttacgccca
     1021 taatatcccg ccttaacgtt atattctccc ggcgttcccg cttctattcc gcaggcatcc
     1081 agccggatgt agctgccacc gccgttaagg gtgattttct tcttcgcggt gatcttgatc
     1141 tcatcttccg tactggtgat cactatcgat ttctgtgcca acagttccat caggtcgttc
     1201 tgtgcctgta ccgagatcgc ccccttgttg gcaaacagtt tgatgcccgc tttacgcaca
     1261 aacacgctca gcgcctgacc cacaccgata aacatattct tcacaacacc gatatcagcg
     1321 tgatttcctg cgttcgcgat caggtttttc gatgcggcca gttgcaggtg ctctccgctg
     1381 gcgatcgcca cgcctttggg tgcgcttagc aggatcgccg cctgcgtcag atcttttaca
     1441 ctctgctgca acagcgctat ttgtgatgac agatctgccg gatcggcatt cgccgtctgc
     1501 gcatcagtgg acagcgactt catctgtgac tgtgcgccat ccagtattgc catcgccgga
     1561 gccatttcgc gcacctgacc ctgcgccttg tcctgcgtat cggcgctgat aaaaatccct
     1621 ttcttcgccc gtactgcccc ccacatatcg gtacgcagtt caaagccttc gccgcgctgc
     1681 tgtttcccgg catccaccag atgaccgagg ttgagctggc tcttgccgcc gtactcggta
     1741 ctgaccttga tatgctcttt gccgcgctca tcgtcgaggc ggattttgtt gttcgccggg
     1801 gttcgcagca cgttgcgctt gtagttctgg atggtcacat ggtcggtgtg cgccgagtcg
     1861 tgctttaccc cggcgatata cggcctgtcc gggttgcctt cctcaaaggc gatgctgact
     1921 tccgttcctg ccagcagcgg caggtgcagg ccgtaggtat ccccggcata gggcctcgac
     1981 tggcgtaccc acaggctctc atagcccggt ttccacgtat cgcggtcaaa gtcgaggttg
     2041 acccggtagc gcccgtcctt gtcgatatgg gcgtagatgt cgttcttcac cgtgctggtg
     2101 acccgcgccg ggagcgtgcc ggccatcacc gggcggggga taagcgccgg gcggtagccg
     2161 tagcgctctg agtacggtat cgccgtgaac gtcagttcat agctgcggtc acgcgccgcg
     2221 ctggccgtca cgccggtaat cagcacgccc ttgcggaaca ccgcaggcgc gtcgtcgccc
     2281 tgaaccctga tttccagccc cggcatcagc agtgaactgg tgctctggcc tttaagaatg
     2341 gcctgttcat tcaggtaacg ttcgtgacgg atacgggcgt aaaacgcgcc gctttccgct
     2401 acctctttat cgcctttttg caggaagttg tcggcatagt gataggcttc gccgtaggtg
     2461 gttttatcgc ccccggtggc gtcatgctgg cctgtggtca tttccgccgt ggcttcacgg
     2521 tagttgtagt cccgcgtggt gacgcttttt tccaccacgc tgtatgcggt gttcagcccc
     2581 catacggctt ccgtggcgct gtcgtgcagc cctgaaggat ggcgaagcgg cagcgtcagt
     2641 ccgcgctcat agccgctctg gtcgtcgaaa aactcgataa cctcaatttt cagccgcgcg
     2701 tcagtggcga agcggaacca gatacccact tccgacagca ggcgtgaaat aaatgtcagg
     2761 tcgtcctcgc catactgcat cacctgttcg cgggccgggt attcgctttt gaggttaaac
     2821 acgaagtcct gaccgcgcat ctggtgccgc tcgcgcagga ttttttcgac gatttgcggc
     2881 accgtcagat tctggtaaat ggcgttctga cggctgcggg ccagcagcac catacggggt
     2941 tcaaggcgca cttcgtaacg ggcttcgtcc tgtgacgatg agagatgttt aaagccggta
     3001 atgacgccgt gcagggtgcg cagggcagtc tgtaccggca ttccctgtac cggcggtgcc
     3061 gtcaggctga atgcgccgtc ctgcatcagc accgactccg gcgttattgc tttatcgcgg
     3121 ctggtaaact gaatgtcgta gcggaagggg gagctgagct gttcgtggcc tgtaaaagtc
     3181 agcacgtcca ccggactttg caggccacgg attttgagtt tgtggtgtga gtggtcaaag
     3241 tttagggcag gaatattcat cgttattttc ctgtatagat agaaatactc tcatcattca
     3301 aaataaatca acaatgaaac tcaatgaact ctcgccattg tggtgtcaca ttatttctca
     3361 ctaagaaatc gtttctttca tcagacgata gactgtgcca gaacggagac cagatatcgc
     3421 cgtgaacgtc agttcatagc tgcggtcacg cgccgcgctg gccgtcacgc cggtaatcag
     3481 tacgcccttg cggaacaccg ctggtgcgtt gttgatttag ttatcgtaca atgagtgaac
     3541 atcactccca tcaatatcaa tcacaacttg attagttcta atgttaaaat acattttctc
     3601 aatccctcca ttcaaaccaa atcgccacaa aaaatattca atattaattg aatcctcatc
     3661 ttcagacgca caataaccat tatctttttg aaaaagataa tctccgccta gtcgactact
     3721 gcatatgcca acccgaaact tcattatatc aaacaacgat ctatatattt ttttcttcct
     3781 aatccatctg acatcagtaa aataatactc caaagaacca atatcataac gatgctctcc
     3841 tggtaaaaaa acatacgcat cattaagaat gaaactaaag acgcgtgatt gtcttgccgg
     3901 aatttcaatc caatcatgat cgaaattata ttcatcgaac tcacatttac tggacagata
     3961 atcaagaagt acattatttg tagtaatagc aaatgttttt ccacacatcg aaga
//
LOCUS       DAZFRE010000210         3965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFRE010000210 DAZFRE010000000
VERSION     DAZFRE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.215"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..63
                     /locus_tag="ACHKUI_005010"
     CDS             <1..63
                     /locus_tag="ACHKUI_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO6601015.1"
                     /translation="YLTNLYFKIKEDRRKAARGE"
     gene            68..403
                     /locus_tag="ACHKUI_005011"
                     /pseudo
     CDS             68..403
                     /locus_tag="ACHKUI_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            995..1528
                     /locus_tag="ACHKUI_005012"
     CDS             995..1528
                     /locus_tag="ACHKUI_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6601016.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1685..1867
                     /locus_tag="ACHKUI_005013"
     CDS             1685..1867
                     /locus_tag="ACHKUI_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601017.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1882..2013
                     /locus_tag="ACHKUI_005014"
     CDS             1882..2013
                     /locus_tag="ACHKUI_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601018.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVALFLSMAWMMLSSSGLLG"
     gene            2016..2483
                     /locus_tag="ACHKUI_005015"
     CDS             2016..2483
                     /locus_tag="ACHKUI_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6601019.1"
                     /translation="MNRMLSAFIVILLVACGALWMATDHYRDNAITYKAQRDNKASEL
                     KLANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
     gene            2794..3120
                     /locus_tag="ACHKUI_005016"
     CDS             2794..3120
                     /locus_tag="ACHKUI_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO6601020.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            complement(3243..3422)
                     /locus_tag="ACHKUI_005017"
     CDS             complement(3243..3422)
                     /locus_tag="ACHKUI_005017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601021.1"
                     /translation="MVSKSKAHCRRMLQALQQTRAGIFDQLENCQHTLPEYIAISSET
                     SATLIHRVPPEKKKK"
BASE COUNT         1021 a          821 c         1106 g         1017 t
ORIGIN      
        1 tacctgacta acctgtattt caaaatcaaa gaggaccggc gtaaggcggc gcggggagag
       61 taaagtgatg aataaaaaat atgaactggt tgttaagggg ataaataatt acggggataa
      121 ggttactgtt actgtgaagc cggaaggtga cgggcaagcg tcgctgttgt tgccagatgt
      181 ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag ctgaggcgaa
      241 aaagcaggcg aagcagtttt tcatggatgt tgctgccggg ttatgtgaat ggaacgaacc
      301 gttgccggaa aagcgccccg taattttaga ggcgcaggat gtgttgataa cctacaaagg
      361 aaagctaccg ggaagaatta cttgttctct gaagatgccg ccgtcaacac tgcggtcaga
      421 aaaagatgat gttgaatcac gtattgaaaa actggagagc tacgtcgttg agctgaataa
      481 gaaatggtcg atattggtgc cttctggcga tgaaaagcag tttgctgcgt ttgacgatta
      541 ttgtcggaaa gtgatgagca gaaatctcgc agagtgtttc agtattcata atgataattt
      601 cagtgagctg gaatgggagt gtaaccggcc atcctttgtt gtatccggtg atgctgggaa
      661 aataaccatc tcagaaaatg ggaaagtaac acctccatcg caccagcaca gtgaggagct
      721 cattgaattt gccattgatt acctgaagaa caataaaaag caggggctga tgaagcgcgt
      781 tggccgttgc atgggatatc ttcaggtagc cgctgagatt gaagcgctgg ccagtggtgc
      841 tgataaggat gcaattgtgc gggaggctct tcttcgtgat tttaatactc caccctttaa
      901 aaaagtgccg gcttactggc ttcatccggg gctgacttat cttaaagtgc gtatttagtg
      961 ggccagggac agcggctgaa tatttaatat atccatgaac accaaaatca aatacggcct
     1021 gtcggctgcc gttctggcgc tgattgccgc tggtgcgcct gcgcctgaca ttctcgacca
     1081 gtttctggat gaaaaggaag gcaaccacac cacagcatac cgtgatggtg caggtatctg
     1141 gaccatctgc cgtggtgcca tcatggtgga tggcaaacct gtcgttccgg gcatgaagtt
     1201 gtcgaaggaa aaatgcgacc gggttaacgc cattgagcgt gataaggcgc tggcatgggt
     1261 ggagaaaaac atcagagtgc cattgagtga accccagaaa gcggggatcg cgtcattctg
     1321 tccgtacaac attggccccg gtaagtgttt tccgtcgacg ttttataaac gaattaatgc
     1381 aggtgatcgc aggggagcgt gtgaggcgat tcgctggtgg attaaggacg gtggcagaga
     1441 ctgccgtatt cgttcaaaca actgctacgg tcaggtatcc cgtcgtgacc aggagagcgc
     1501 gctggcgtgc tggggtatcg acagataagc agaatatttt gctgaaaaat aaggtatggc
     1561 tacgcgggcg gataacacga aatcctgcga actggcgaaa cgtaagtgaa taaaagtaaa
     1621 aaccccgttt gttggcacca agcggggttt tgtgtttctg accttgagta aggcaaggga
     1681 gaacatggcg aagtataaac gaattctgtt gaggttgact atgaaaaatg gccttgaact
     1741 gaaagcgcct gtaactgatg acatcagcag agcactggct tttgccatta agtgggtggc
     1801 ggtcggtgtt gctgtgtccc cgatgctgta tgggctggca aaactggtca ttgcgttgaa
     1861 atcgtgaagg gaggattaag catgtcagac aaactcataa cgctggcgaa gatcctctgt
     1921 gtaattgtcg gcatttcatt ttcactaatg ctggttgctc tttttctttc catggcctgg
     1981 atgatgttgt cttcgtcggg gttgctgggg tgaacataaa ccgaatgctt tccgcgttta
     2041 tcgttattct gctggtggcc tgtggtgcgc tgtggatggc aacagaccat taccgtgata
     2101 acgcgattac ctacaaagcg cagcgcgata acaaagccag tgaactgaag ctggcgaacg
     2161 caaccattac tgatatgcag gtgcgccagc gcgatgttgc tgcgctcgat gcaaaatact
     2221 cgagggaatt agccgatgcg agagctgaaa atgaaactct tcgcgctgac gttgccgctg
     2281 gtcgtaagcg cctgcggatc aacgccacct gtccaggctc cgtgcgtgaa gcccccacca
     2341 cctccggcgt ggataatgca accggccccc aactggcaga caccgttaca cgggattatt
     2401 tcaccctcag agagcggctg atgacgatgc acaagcaact ggaaggggca caggactata
     2461 tccgcactca gtgcctgaaa taagttttgt tgatgcgccg tatcgtcgct atattccctc
     2521 attaacagag accgcagccc gacagggaga ctcctctgcg cgagtgtgcg gggataatta
     2581 aaaacgatgc acaccgggtt tttaccgcgt taatgattcg cgggtttatc ccggtgcgat
     2641 ggtggaagaa acaggaagct gtattacaga aagtgctact actgtatccc gatgcgatgt
     2701 atgtaatgtg agtcagataa tggcacagga tgtggtgatg tggcagtctg gaacacagga
     2761 tatattgtca gaataagacc cgtaggaata aaaatgaaaa gacgcctttt actacttttt
     2821 ctgttatctg tcctggcagt gggatgctcg cagcaaaaag ctgatgagcc ccggcaatta
     2881 gtgacggtgt atccacgata tccggaatat gctgcagcaa attatatcaa ggggctggtt
     2941 gaggttaagt tcgatattgg tgctgatggg actgtgacac ggatcgtttt tctccgctca
     3001 gagcctcata atttgtttcg tgatgaagtg gtgaaggcca tggcgaaatg gcgatttgaa
     3061 aagaatcgcc cctgtcaggg agtgaagaga caatttatct ttacgccgtc acgtccttga
     3121 tgcttccagg tagagagggg ctggacgcag gagaaaaatg aaagagccag cggttatatt
     3181 tttgtcatgg ctgacgagga atgatggaag aaggcgttgt atgccacaca acgcctcact
     3241 gttcatttct tctttttctc tggtggaacc cgatgaataa gagttgcact ggtttccgat
     3301 gagatggcga tatactcggg caaagtatgc tggcagtttt ccaactggtc aaaaatacct
     3361 gctctcgtct gttgcaatgc ctgcagcatg cggcggcaat gcgccttgct tttactaacc
     3421 atctttcctt cctctatcag tcgctgcgtg aactcatcat gtaataccag gtaaatgcgg
     3481 atgttatcgg ttttggctac gcagcatagt acaaaacgga caggtgcatc ccgggacggg
     3541 ggaggcgtca catgtccctg tgatggttgt tccgggtaat gcactgtgtg gggcataaaa
     3601 atgtccgata attttacttt ctaccgcagt tagttgattc gttggtcctg gtagcacatt
     3661 gggcgaggat ttaaatgcca ggcaactgaa ggatgatgtt gcaagggaga tagcgagaat
     3721 atttctgatt ttcatttgat gatgcctctg tgtgaaatga cggtaaacga cgcacttgtg
     3781 ccggcacata atagcaagca ccataataga tcagattcga ttcttgctgt aagtgataat
     3841 tattctcgtt ttcgggtcct ttccgtcgat ccaacaggtt acggggcggc gacctcgcgg
     3901 gttttcgcta tttatgaaaa tttttaggga aaaatcagat ccgtttttct tctttttaac
     3961 tgatt
//
LOCUS       DAZFRE010000211         3957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFRE010000211 DAZFRE010000000
VERSION     DAZFRE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.216"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..626
                     /gene="ykgH"
                     /locus_tag="ACHKUI_005018"
     CDS             <1..626
                     /gene="ykgH"
                     /locus_tag="ACHKUI_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO6601022.1"
                     /translation="FYLKKKIRVILFIIAICMVMVLLFLYINKDNIKVTYSLKINQTT
                     PGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIKLEWSGNKRDLPTAEA
                     EISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCV
                     TISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(869..1564)
                     /locus_tag="ACHKUI_005019"
     CDS             complement(869..1564)
                     /locus_tag="ACHKUI_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6601023.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1557..2984)
                     /locus_tag="ACHKUI_005020"
     CDS             complement(1557..2984)
                     /locus_tag="ACHKUI_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6601024.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYCHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2995..3714)
                     /locus_tag="ACHKUI_005021"
     CDS             complement(2995..3714)
                     /locus_tag="ACHKUI_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6601025.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYTKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1037 a         1040 c          827 g         1053 t
ORIGIN      
        1 tattttatct gaagaaaaag attcgtgtta tcctttttat tatagctatc tgtatggtaa
       61 tggtgctgtt gtttctgtat atcaataaag ataatataaa agtgacttac agtctaaaaa
      121 taaaccagac aacaccaggc atacttgtta gctgtgatag caataataac tttgcctgtc
      181 agaccacaat gactgaagat gttattcagc gaattactac attttttcag accagcccag
      241 atgtcaagaa cagagaaata aagctggaat ggtcaggaaa taagagagat ttaccaactg
      301 ctgaagcgga aatatctcgc gtgcaggcgt ctatcatcaa atggtatgcg tcagaatacc
      361 ataatggcag gcaagttctc gatgagatac aaacgccttc agcaattaac agtgagcttt
      421 atacaaaaat gatatacctg accaggaact ggtcactgta tccgaacggt gatggctgtg
      481 tcactataag ctcaccagaa ataaaaaata aataccctgc tgccatttgc ctggctctgg
      541 gattttttct aagtattgtg atttctgtga tgttttgcct tgtcaaaaaa atggtagatg
      601 aataccaaca aaactctggg cagtaataaa cagcattctg acagagagtg cctgtataaa
      661 actatagtga cagtgaccgg gaagggagcc cgtcttcagg aatggattcc tgtcttttta
      721 actgtaaata tcctggcatt attaatgtca ggatatttac agaagagctg cataaccatg
      781 acggaacggg gttaagtcag gttgtcctta acgctattca caacctcgta taccgccttc
      841 aggtttatgt ccagactaca tatctctctc agcaatcctc aataatcaga tacaccgctt
      901 tcaccggacc atgaaccccg acgactttga taagctcaat gtccgccgtt gaactgggac
      961 cactgatgat gttaatacat gacggcattc gttcaccggc ctgcgctttc tgatgcaatt
     1021 tttctgcgag ttgcgctaca cgcggcagga ttgtgctttt acgcaggata aaaagagaag
     1081 attccgggag caggctcaat gaacgcccgc gcccggcggc ggaaaaaaga accacgcctc
     1141 ccgattcggt taaaccatat tcagcatata cgacgcccac tttagcctgc tctgcttgtg
     1201 atatgttctc aatacctttc gccggatccc aaacaacggc attgcattcc tgctgcaaac
     1261 gttcgctaat ccccaattcc tccagcctcg tgtcaccact aatcacgacc gactgatctc
     1321 ccagctcttt acacagacgt attgcagctt ctgccgcctt cgcctcgctg gtcagttcac
     1381 agcgcgtcaa cataacatcg ctggcaaact ggataaacgc gtcacagcgc tgctgttggg
     1441 taagttgggt aagccgctcg ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt
     1501 caagtcgcag cgggcgcccc agtgcctgag caacgttatt caaaaattcg ctccgattat
     1561 ccattctttt tctcctgcgc ctgatgtttc ttaaaccaac tacggaaact ctctccgtca
     1621 gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg cgccaaattt gagtggtgtt
     1681 ttgccgccat tgataaacca gcttgccgca tgagcaccag ccatcatccc aactttccac
     1741 aatcctggat gactattggc ataagcgaac atttttatcg cccgttgctc tgcttttgcg
     1801 gtgatccctt tttcagccat cacccgacga tgacgcaaaa tcagttttga cagcggaata
     1861 cgcaccggac acacgctgtc acaagcggtg cataaagagc aggcgtaggg taaatcttta
     1921 aaatctttat agccgccaag taacggggaa atcaccgcac caattggccc tggataaata
     1981 gagccatatc catgaccgcc aatatggcaa tatgccggac aagtattcat acaagccccg
     2041 caacgaatac agcgcagcac atcccgaaat tcagaggcca gcacctcaga acgcccgtta
     2101 tcgacaataa ccagatgaaa ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct
     2161 gtcagccagg tgttgtatcc cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg
     2221 atcaatacat ctacctctgc aaacgtggga gcaatacgct ccattcccat cactgcaata
     2281 tgcgttttag gcagcgtggt acacattcgc gcattacctt cattggtcac caggcatacc
     2341 gaaccggtct ctgccaccgc gaaattacag ccggtaatac ctatttcagc actgaggaaa
     2401 tcttcgcgga ttttttgccg aatgaataag gtcatcgctt caggcgtttc cggcccctca
     2461 tagcccagac gttcgtgtag cactcgacgg atctgatggc gatctttatg aattgccggg
     2521 accacaacat gagagggcgg atcttgatcc agctggagaa tgtattcacc cagatcggtt
     2581 tcaatcacct gaatcccggc atcctgcaac acatgattga caccaatctc ttcggtcacc
     2641 atcgatttag atttcaccac cttccgggca tttttgcgtt gggcaacctg taaaatgtag
     2701 cgggtggcgt cttctttggt ttttgcaaaa tagacgtgac ctccgttttg cgtcactttt
     2761 tctgagagct ggtacagata agcgtcgaga ttactcagaa catgatcacg tatctgggcg
     2821 gcccgatcgc gccactcctc ccagtgcccc aattcatcga ccattttttg ccgatttgcc
     2881 ccaatacgct gctgcgcgtt tgccaccgct ttacgcatga tcggatcttc aatttgctga
     2941 cggatgcgtg tcttaaaatc tgtattactg gttttgatcg acatctttat atcctcagcg
     3001 gctcatcagc acttcagcaa tatgcatcac tttgactttc tgcccttccc gttgtaatcg
     3061 tccactgatg ttcagcaggc aactcacgtc agcaccaatt aaatactcag ggcggacttc
     3121 catcaggtgc gcaacctttt ctttcaccat ctcgccggat atttcggcca ttttgaccga
     3181 gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca aaggtcaaca gctccagtcc
     3241 acgcacattt ttcagcagcg taagaggttc gtccttcacc cccagcttac gggccaggct
     3301 acaagatggg tgatacaccg ctctcccttg caaactggca cctacatcga ctacccctaa
     3361 tgtattaaca ataaaagagg tgagatcctg catacgcgcg gcaacctttt cggcacgtga
     3421 tgcccattca ggttcatccg ccagatacgt cgggtaactt tttacggcat aggtgcaaga
     3481 gccagccggt gaaataatgg gatcgtcgtt atcctccagt gcggcgatca gatttttcat
     3541 ccctggaatc gcttctttgg tataaccact attgatcgca ggctgaccgc agcatccctg
     3601 tttctccggg aaatttacgc gacagccgag tttttccagt agcagcacgg agtctcgtgc
     3661 cattcttgat ttcagggcgt caccaataca ggtgacaaag aaattgacat tcacaactaa
     3721 tactccatta cttcatgccc atttatgccc gatggttccc cccttccggg ggatcaatca
     3781 tcaataaata tggcgttctt ttttaattgg ctagcaacat accacccctc atttgtatga
     3841 caaatgataa tctttcatct aaacttcaaa tgcaataact aattttattt ctttcggcat
     3901 cgctcacatt ttatagggaa atcgacgatc atttttggca taagaaatct atttaaa
//
LOCUS       DAZFRE010000212         3883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFRE010000212 DAZFRE010000000
VERSION     DAZFRE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.217"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..82
                     /locus_tag="ACHKUI_005022"
     CDS             <1..82
                     /locus_tag="ACHKUI_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO6601026.1"
                     /translation="LVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            169..390
                     /locus_tag="ACHKUI_005023"
     CDS             169..390
                     /locus_tag="ACHKUI_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6601027.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPNVRLMSVTLN"
     gene            470..838
                     /locus_tag="ACHKUI_005024"
     CDS             470..838
                     /locus_tag="ACHKUI_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6601028.1"
                     /translation="MSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSNELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            928..1305
                     /locus_tag="ACHKUI_005025"
     CDS             928..1305
                     /locus_tag="ACHKUI_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6601029.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            1302..1790
                     /locus_tag="ACHKUI_005026"
     CDS             1302..1790
                     /locus_tag="ACHKUI_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6601030.1"
                     /translation="MKLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYS
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            1802..1999
                     /locus_tag="ACHKUI_005027"
     CDS             1802..1999
                     /locus_tag="ACHKUI_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6601031.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQARQDIRTLRHLQLQHPNR"
     gene            2092..2958
                     /locus_tag="ACHKUI_005028"
     CDS             2092..2958
                     /locus_tag="ACHKUI_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6601032.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTGFRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGVHLITGQQADRVADLERMLHLFSGRPIPDNR
                     ENIIIRLDDHIQSVQGQECYEDEMFSIRYFKKGSAHITFRKPQLVDRLNDIIARHYPE
                     ALASTVNKSRKT"
     gene            3030..3293
                     /locus_tag="ACHKUI_005029"
     CDS             3030..3293
                     /locus_tag="ACHKUI_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6601033.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            3290..3616
                     /locus_tag="ACHKUI_005030"
     CDS             3290..3616
                     /locus_tag="ACHKUI_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6601034.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQL"
BASE COUNT         1039 a          997 c          948 g          899 t
ORIGIN      
        1 cctggtggat atccgggtac cggaccatct gatagtcggt ggcagccagg ttttctcctt
       61 tgcggaacac ggtctgcttt aacccgtcac cgtcacaatc accttcatat cacttcagtt
      121 tctctttctc agctgtttct tactttcaca ttcaggagga ctattctcat gaaaatcatc
      181 acccgtggtg aagccatgcg tattcaccgt cagcatcctg catcccgtct ttttccgttc
      241 tgtaccggta aataccgctg gcacggtagc acggatacat ataccggccg tgaagtacag
      301 gatattcccg gtgtgctggc tgtgtttgca gaacgccgta aggacagttt tggtccgaat
      361 gtccggctga tgagcgtcac cctgaactga gtctggaagg gccttcagaa gagcagaatt
      421 atcgccacca ccggaccatt cttaaccgat tttctgtgag gattttatcg tgtcagacac
      481 actccccggg acaacgcatc ccgacgataa caacgaccgc ccctggtggg ggctaccctg
      541 caccgtgacg ccctgttttg gggcatgtct ggtgcaggag ggtaaccggt tgcattacct
      601 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
      661 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcaatg aactgaatcc
      721 ccgccatcag cataccgtga cactgtatgc aaaagggctg acctgcgaag ccgacaccct
      781 cggcagttgt ggctacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaact
      841 ctccagaata accttctgct ccggcctggt gttttcacca cgccactttt ccatttttca
      901 tctctgcata tcaggaaaat cttcagtatg aaaacattac ccgacacgca tgtacgggag
      961 gcatcgcgct gcccgtctcc cgtcaccatc tggcagatac tgctgtcccg actgctggac
     1021 cagcactacg gcctcacact gaatgacaca ccgtttgccg atgaacgtgt gattgagcag
     1081 catattgagg caggcatttc actgtgcgat gcggtgaact ttctcgtgga aaaatacgca
     1141 ctggtacgaa ccgaccagcc gggattcagc gcaggaactt cgtcgcagtt aatcaacagc
     1201 attgatattc tccgggctcg cagggcgacc ggcctgatga cccgcgacaa ttacagaacg
     1261 gtaaataaca ttacccgggg gaagcatccg gaggcgaaac aatgaaactt tccctgacgc
     1321 tggaagcaga cagcgttaac gtgcaggcgc tgaacatggg acgaattgtc gttgacgtcg
     1381 atggtgttaa tctcgctgaa ctgattaacg tggcctgcga caatggctac tcccttcgta
     1441 ttgttgatga atctgaccgg acatcagcag agcgcacgcc accatttgct gccctgaccg
     1501 gcatacgctg cagtaccgct catatcacgg caaaggacaa cgcctggttg tattcgctgt
     1561 cacatcagac aaatgacacc ggcgaatcag aatggattca tttcacaggt agcggatatc
     1621 tgttacgtac cgatgcgtgg tcatacccgg ttttgcggct taagcgccta ggcctgtcaa
     1681 aaacgttccg ccgtctggtt gtcacactta cccgacgtta tggcgtcagt ctcattcatc
     1741 tggatgccag cgctgaatgt ctgccggacc tgcccacttt cgactggtaa ccaggaacaa
     1801 catgaaatca ttaacaacgg aaaccgcact ggatattctg attgcgtggc tgcaggacaa
     1861 tattgactgc gaatcggaaa ttatctttga caacgatgag gataaaacgg attcggcagc
     1921 actgctgccc tgtatcgaac aggcgcgaca ggatatccgt accctgcgtc atctgcagct
     1981 tcagcacccg aaccggtgag tctgactcaa tcatctcacc acacttcatt cccccaacgt
     2041 cagcctgaac agggctggcg ttttcattta tctgcaaaag ggatttcgat tatgtctgaa
     2101 atcacagcct ctcgtccgga agtggtcaac gggcatacgg acgttatctg ctcaaccagt
     2161 atccgccaca ttctggctgt acgaaagagt gcgctgctgc aaatcgacac acttatccgg
     2221 caactggcag aaatctcagc aatgacagaa agcatcggag gaaaaaccgc actggactgg
     2281 gcgatgaaac aggattttcg ctgcggctgc tggctcatgg agaaaccgga aaccgcaatg
     2341 aaagccatca cccgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     2401 atgctgtcat taatggatgc gcaggcccgt gatacagggt tcaggtcact ggagtacgat
     2461 aactttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccagaat
     2521 aaggatgagg tgtttgagcg aggggtgatc aacgtcttca ggggactgag ctggaattac
     2581 aaaaccaatt gcccctgcaa atttggcagt aaaattatcg tcaacaacct ggtgaggtgg
     2641 gaccggtggg gggttcatct tatcaccggg caacaggctg accgggttgc tgacctggaa
     2701 aggatgctgc acctgttcag cggcaggccc atccccgaca accgggaaaa tatcatcatc
     2761 cgtctggatg accacattca gtccgttcag ggtcaggagt gctatgaaga tgagatgttc
     2821 agcatcagat actttaagaa gggctccgca cacatcacgt tcaggaagcc acaactggtt
     2881 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     2941 aaaagccgca agacataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     3001 gatagtaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     3061 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     3121 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     3181 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     3241 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     3301 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     3361 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     3421 atcgccagta ctctacagag aaagacccga gttaggggct gggatcagag gttgttcgta
     3481 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     3541 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     3601 tttctggcag ctataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     3661 ttttataaat ctctgtattt tgttgttact tcgattccgc gattcagcat atcgaaccag
     3721 acaaaatgat tacaacaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     3781 gcacatgggg gcctcattgg tggccccaaa aagaacaaag acaaaacagt cgatacaaaa
     3841 caattggtta tataaccagt ttcattccga gtccgggcac caa
//
LOCUS       DAZFRE010000213         3825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFRE010000213 DAZFRE010000000
VERSION     DAZFRE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.218"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..865)
                     /locus_tag="ACHKUI_005031"
     CDS             complement(<1..865)
                     /locus_tag="ACHKUI_005031"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6601035.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKL"
     gene            983..1380
                     /locus_tag="ACHKUI_005032"
                     /pseudo
     CDS             983..1380
                     /locus_tag="ACHKUI_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1526..1951
                     /locus_tag="ACHKUI_005033"
     CDS             1526..1951
                     /locus_tag="ACHKUI_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6601036.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1948..2298
                     /gene="tnpB"
                     /locus_tag="ACHKUI_005034"
     CDS             1948..2298
                     /gene="tnpB"
                     /locus_tag="ACHKUI_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6601037.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            2329..3426
                     /locus_tag="ACHKUI_005035"
                     /pseudo
     CDS             2329..3426
                     /locus_tag="ACHKUI_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3426..>3825
                     /locus_tag="ACHKUI_005036"
     CDS             3426..>3825
                     /locus_tag="ACHKUI_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6601038.1"
                     /translation="MNDISSDDIFLLKQLLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGED"
BASE COUNT         1014 a          987 c          915 g          909 t
ORIGIN      
        1 caagttttga taatacatcc agattctttt cctttaccgg agaaaaagga cttctgcata
       61 caggagtatt tataatcccc atatagtaaa gaagagtttt tatacctgcc aggacaccac
      121 tctgaataag agtgtcaata acctggttac aggctacttg catttccttc gccttaatca
      181 catcattgtt tttgactgct tcaaatattt caagataacg ccaccccatt atattatatg
      241 tactaccgat tcccccatct gcacctgcaa ttaaccctga tgcaaatatt tcatcgtaac
      301 cattatataa cacaagctct ggatgattcc ttttgatttg ttccatctgg aataaatcac
      361 cggatgtctg ttttaatgca caaaccctgg gtattgtgac aagctcattt atctgatcta
      421 aagaaaaacg cactccgcta agagccggta tattataaac taccatcgga agtccatctg
      481 cagaatcaat aatcttcctg taatggatac aatgctcttc aaatgaaaaa ggataataaa
      541 atggagtaac agcagatata gcatgatatc cgtatttttt tgcagctttt gcaagtacct
      601 cgctttctgc cgtactgatt ccgcccacat gcgctataag tgtcagtctt ccatctgatt
      661 catcggctac tgtttcaaga attttttctc gctctgcaac attctgaaga aatgcttctc
      721 cagtagagcc tccaacatac aatcccttaa tattttgttc gatattaaaa cggatcagtt
      781 ttcgaagact ctccatatcg atttgctggg attggtcata aggagtgggt aatgctgata
      841 tgaccccttt aaattcacat tgcattttta atcaccttca cttaactgca taatgggata
      901 cctagtacac catcaagtac aaagattatc caacagaaaa gaagcacaat gtgaagatta
      961 tcaaaaaaaa accatcagaa agctgtcatg ccgctcagtt aaggatcggt tgatcgatcc
     1021 agtgtccagt tactattctc tgcgtaaccc tctgcgacta tcttgccccg gaactgatta
     1081 cccacagccc tgaagagtcc gacactgtca cgctacgcaa acggcgtctt cctctggaaa
     1141 tgatggcctg gtgaattatc agtatggccc atgagcgtaa ggaaccttca ccagattgtg
     1201 aaccggctgg acacatgctg ccaggtagtc gcccctttgt cgcgccgtga tccatgccca
     1261 gcagggccag gaagcggggc cgtacgccac gtgttcacgt aaacagcgca gccctgacat
     1321 aacaccacgc tgtatccgct ctggtgcaat atgaacctgc tgaccatcga tggcgtgttc
     1381 taactaacac aggacacgcc agaacacgac ataaagcttc actcgccaga tacatggtaa
     1441 gcgtaaactg accgccgtat gtagccatta agccagtatt ggtaacgtag gtgcccacct
     1501 tttcatcaac tagtggacac ctgttatgga acagaaaata ttatccgcag aaccccgcag
     1561 aacattttca aatgagttta aacttcaaat ggttaaactg gcttcacaac taggagcctc
     1621 tgttgcccgt attgccaggg aacacgatat caatgataac ctcctgttca aatggctcag
     1681 gctctggcag aacgaagggc gtatatcgcg gcgtcttccg gtaacaacct cttctgacgc
     1741 tggcgttgaa ttattacctg ttgagataac gcctgatgag cagaaagagc ctatggcggc
     1801 tcttactccg cttttatcca ctccttcaca aagcacagtc agtgccagtt cctgtaaagt
     1861 tgagttccgc cacggtaaca tgacgctgga aaatccttca ccagagctgc tcacggtgtt
     1921 gatccgtgaa ctgaccggga gggggcgatg atctcgctcc catcagacac tcgaatctcg
     1981 ctggttgccg gcgttactga tatgcgtaaa tccttcaacg gtctgggtga acaggtacaa
     2041 catgtgctgg atgagaaccc cttctccggt cacctgttca tcttccgtgg ccgacggagt
     2101 gacatgatta aaatcctctg ggctgatgct gatggtctgt gcctgttcac caaacgcctg
     2161 gaggaaggcc tgtttatctg gcctgcggtg cgtgacggta aggtatccat tacccgctcg
     2221 cagctggcaa tgctcctcga taagctggac tggcgtcagc caaaaacatc ccgtcttaac
     2281 gcactgacaa tgttgtaaaa atgtcatggc cggattataa aaacggtcat gagtcggaaa
     2341 tacctcattc gtatcactga gctggaaagg ctgctctctg agcaggctga agccctccgt
     2401 cagagagatc tgcaactgag tctggttgaa gagacggagg ccttcctgcg ctctgcactg
     2461 gcacgtgccg aagaaaagat cgaagaggag gagcgggaaa tagaatatct gagggcccag
     2521 atagaaaaac tgcgccggat gctgttcggt acccgttctg aaaaactgca gcgtgaggtt
     2581 gaacaggctg aggcccaact gaaacaacgc gaacaggaaa gcgatcgtta cagtgggcgt
     2641 gaggatgacc cgcaggttcc ccgccagttg cgacagtcac gtcatcgtcg tccgttacca
     2701 gcacatcttc cccgtgaaat acaccgcctg gagcctgaag aaagttgttg cccggagtgt
     2761 ggcagtgagc tggattatct gggtgaagtc agtgctgagc agctggaact ggtgagtagc
     2821 gccctgaaag tgatccgcac tgttcgggta aaaaaagcct gtacaaaatg tgactgtata
     2881 gttgaagcgc cagcgccgtc ccgcccgata gcgcgcggca tcgcgggctc cggattactt
     2941 gcccgcgtgt taacgggaaa atactgcgaa cacctgccac tgtatcgtca gagtgaaatc
     3001 tttgcccgac agggtgccga actgagccgt gcattaatct ccaactgggt tgacgcgtgc
     3061 tgccagttaa tgactccgct aaatgatgcc ctgtaccgtt acgtgatgaa cacccgcaag
     3121 gttcacactg acgacacacc ggtaaaagtg ctgacaccgg gcagaaaaaa ggcaaaaaca
     3181 ggacgcatct ggacgtatgt ccgggatgac cggaatgcgg gctcatcaga gccaccggcg
     3241 gtctggttcg cctactcacc agacaggcag ggaaaacatc cggtacaaca ccttcgtccc
     3301 ttccggggta tcctgcaggc ggatgcattc agcggttacg atcggctgtt cagtgccaaa
     3361 cgtgaaggtg gtgcacagac agaagttgcg tgctgggtcc atgcccggcg aaaaatacac
     3421 gatgtatgaa cgacatctct tctgacgaca ttttcctgct gaaacagctc ctggccgaac
     3481 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
     3541 atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     3601 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa gagagcgata
     3661 cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     3721 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt
     3781 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggata
//
LOCUS       DAZFRE010000214         3688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFRE010000214 DAZFRE010000000
VERSION     DAZFRE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.219"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..312
                     /locus_tag="ACHKUI_005037"
     CDS             <1..312
                     /locus_tag="ACHKUI_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFO6601039.1"
                     /translation="SSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYPQTVLDS
                     VLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGGST
                     L"
     gene            309..3131
                     /gene="traG"
                     /locus_tag="ACHKUI_005038"
     CDS             309..3131
                     /gene="traG"
                     /locus_tag="ACHKUI_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO6601040.1"
                     /translation="MNEVYVIAGGEWLSNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRTSAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            3147..3632
                     /locus_tag="ACHKUI_005039"
     CDS             3147..3632
                     /locus_tag="ACHKUI_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO6601041.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLAGWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITIPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
BASE COUNT          968 a          805 c         1008 g          907 t
ORIGIN      
        1 tccagcagcg tggtttacca gctgacggac tatatcggct acgacatcat gctccagtac
       61 attcaggaac tgcttcagca ggcccgggcc atgattgcca ccgggaacta tccacagacc
      121 gtgctggaca gtgtgctgga gaatctgaat caggcacagc aacagattgc ggttttccag
      181 gggcaggtaa aggtccagca ggatgcattg ctggttgttg accgtcagat gagctacatg
      241 cgccagcagc tttccgcccg catgctcagc cgttaccaga acaactatca cttcggaggg
      301 agcacgctgt gaatgaagtc tatgtgattg ccggtggtga gtggttaagc aataacctga
      361 acgccattgc cgcctttatg gggacccgga cgtgggattc catcgaaaaa attgcgctca
      421 cattgtctgt tctcgcggtg gccgtgatgt gggtacagcg gcacaacgtg atggatttgc
      481 tgggctgggt ggccgtgttt gtgcttatca gcctgctggt taatgtccgt acatcggtgc
      541 agattattga taacagtgac ctggtcaaag ttcaccgggt ggataatgtg ccggtcggac
      601 tggcgatgcc actttcactg acgacccgta tcgggcatgc aatggtggcc agttacgaga
      661 tgatattcac ccagccggac agtgtcacct acagcaaaac ggggatgctg ttcggcgcag
      721 aactggtatc aaaaagcacc gactttttgt cccgcaatcc ggaaatcgcc aatctttttc
      781 aggattatgt gcagaactgt gtgatggggg atatttacct gaaccacaaa tacacgctgg
      841 aggagctgat ggcctccgct gatccctaca cgctgatttt ttcccgcccc agtccgctgc
      901 ggggcgttta cgacagcaat aataatttcg tgacctgtaa ggacgcgtcg gtttcgctga
      961 aagacaagct gaatctggac acacagagtg gcggaaaaac ctggcattat tatgcccagc
     1021 agttatttgg tggtcgccct gatccgaacc tgttgttcag tacgctgatt ggcgacagtt
     1081 acagttactt ctatggctca agtaaatcag ccagccagat catccgccag aatgtcacca
     1141 tcaatgcgct gaaggagggg atcaccagtt atgccgcacg taatggtgat tcggccagcc
     1201 tggtgaatct ggccaccaca tcatcgatgg agaagcaacg tctggcacat gtctccatcg
     1261 gtcatgtggc gatgcggaca ctgcccatga cgcagaccat cctgacgggg atcgccatcg
     1321 gtattttccc gctcctggta ctggcggctg tcttcaacaa actgacgctg tccgtcctga
     1381 aaggctatgt gtttgccctg atgtggttgc agtcatggcc aatgttgtac gccattctga
     1441 acagtgccat gacattttat gcgaagcaga acggtgcacc ggtggtgctc tcagaaattt
     1501 cacagataca gctgaaatac tccgatctgg cttccactgc cgggtatctt tccatgatga
     1561 tcccgccatt atcctggatg atggtcaggg gactcggggc cggattttcc agtgtgtaca
     1621 gccatttcgc ttcctctgct atcagcccga ccgccagtgc ggcaggcagt gtggttgacg
     1681 gtaattactc ctacggcaac atgcagacgg aaaacgtgaa cggcttcagc tggagcacca
     1741 acagcaccac gtcgtttggt cagatgatgt accagaccgg cagtggcgca actgccacac
     1801 agacccgtga cggtaatatg gtgatggatg cgagcggggc gatgtcccgg ttaccggttg
     1861 gtatcaatgc aacgcgtcag attgcagcgg cacaacagga aatggcccgg gaggcgtcga
     1921 acagagcaga aagcgccctg catgggttca gcagcagtat tgccagtgcc tggaactcgc
     1981 tcagccagtt tggttctaac cgggggagca gtgattctgt cacaggtggt gctgacagca
     2041 cgatgagcgc acaggactcc atgatggcca gccgtatgcg cagtgcagtg gaaagctatg
     2101 cgaaggcgca taatatcagt aatgagcagg cgactcagga actggcatcc agaagtacga
     2161 gaacttctgc cgggatgtat ggagatgctc atgctgaatg gggggttaaa cctaaaattc
     2221 ttggtgttgg tggaggatta ggcgttagag gtggtggtcg ggctggtatt gattgggaag
     2281 ataatgatgc gcatacggca agtagtaata ctcagtcgag ccataatgct cgtcatgata
     2341 tcgatgccag agctacacaa gactttaaag aggccagcga ttactttacc agtcgcaagg
     2401 tcagtgagtc cggcagccat actgacaata atgctgattc ccgtgtggat cagttgtctg
     2461 cagccctgaa ttcagcgaag cagagttacg accagtacac gacgaatatg acccgcagcc
     2521 atgaatatgc tgaaatggca tcccgtactg aaagcatgag tgggcagatg agtgaagacc
     2581 tgtcgcaaca gtttgcacag tatgtaatga aacacgcgcc acaggatgca gaagctattc
     2641 tgacaaacac cagttcaccg gagattgcag aacgtcgtcg ggcgatggcc tggtcttttg
     2701 tgcaggaaca ggtacagcct ggtgttgata atgcctggcg ggaatcccgt ggtgatattg
     2761 gtaaaggaat ggagagcgta ccttcgggtg gtggcagtca ggatattatt gctgaccatc
     2821 agggacatca ggccattatt gagcaaagaa cgcaggacag taatatccgt aatgatgtaa
     2881 aacatcaggt tgataatatg gtcacagaat ataaagggaa tataggtgat actcagaaca
     2941 gcgtacgtgg cgaggaaaat attgtaagag gacagtattc tgaactgcag aatcaccata
     3001 aaacagaggc attatctcag aataataaat ataatgaaga aaagtcggct caggaaagaa
     3061 tgcccggggc tgacagtcca caggaactta tgaaaagggc aaaggaatat caggataagt
     3121 ataagcaata atgctgtttt tgggagatga taatgagatg tttaacccat attactctgg
     3181 taacggtgat acagtttatc gcctgttatc tggctggttg ggggaatgcc gaaacaattt
     3241 ttatgctgtt cttcatagta ctatggcagg gactgtttat atggttgttc tctcaaatac
     3301 gaaaaaaacg gaatgtgtcg gatgagttta agtttagtaa aggcgtttgg tatataacga
     3361 taccagtatc ttccttactc tctccattat taagcctgat ggtttttatc attggtacac
     3421 tatacgaatt aaggcgagtt agtggttgtg taagcgtaag agaatggatg caatctcagg
     3481 taaatgaaca aactaatgaa gatttgcatc tggattttga taatatggat tttcatcgta
     3541 ctaatcctgc aacagggtta cctatgtcag gtattggtgt ggatatcggt ggtaattcat
     3601 atggttatag ttcaaaacga tatgatgagt gaatcttaat ttgtatatta tgacgtttta
     3661 ttcaatatga aggaacattg atgaaaat
//
LOCUS       DAZFRE010000215         3601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFRE010000215 DAZFRE010000000
VERSION     DAZFRE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.220"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            240..2174
                     /gene="yeaG"
                     /locus_tag="ACHKUI_005040"
     CDS             240..2174
                     /gene="yeaG"
                     /locus_tag="ACHKUI_005040"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6601042.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2287..3570
                     /locus_tag="ACHKUI_005041"
     CDS             2287..3570
                     /locus_tag="ACHKUI_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6601043.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQDGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT          972 a          868 c          908 g          853 t
ORIGIN      
        1 ggatgagttg ttgatatgcc attgaaatta agaaagccgt gcaggcaagt tttccatttg
       61 ccatctacgc ttaattttga aggtgtatca ccgggcacgt tgttctcatc gtcaataaaa
      121 tggcatgaga gttgctgtgt tttagcaaga gacgtcgttc agtttacctc ttccgggagc
      181 ctcaactatt catatgaacg gctcttaacc tgtgctaaaa aacgaaagga cggcatacca
      241 tgaatatatt cgatcactat cgccagcgat atgaagctgc caaggacgaa gagttcacac
      301 tgcaggagtt tcttaccact tgtcggcaag atcgcagtgc ttatgccaac gcggctgagc
      361 ggctattgat ggctattggt gagcctgtca tggtcgatac agcccaggaa cccagacttt
      421 ctcgactctt ttctaaccgg gtcattgcac gttatccggc gtttgaagag ttttacggca
      481 tggaagacgc gattgaacag attgtatctt atctgaaaca cgcggctcag gggctggaag
      541 agaagaaaca aatcctgtat ctgctggggc ctgtgggtgg gggtaaatca tcgcttgctg
      601 agcgactgaa atcattaatg cagctcgtac cgatttatgt attgagcgcc aacggtgagc
      661 gtagcccggt caacgatcat ccgttctgtc ttttcaatcc gcaggaagat gcgcagattc
      721 tggaaaaaga gtatggcatt cctcgccgtt atctcggcac catcatgtcg ccgtgggcgg
      781 caaaacgcct gcatgaattt ggtggcgata tcactaagtt ccgggtagtg aaggtctggc
      841 cgtcaattct gcaacaaatt gctatcgcca aaacagaacc cggcgatgag aacaaccagg
      901 acatctccgc cctggtaggg aaagtcgata ttcgtaaact cgaacactac gcgcagaatg
      961 acccggacgc ctacggctat tccggtgcgc tgtgccgcgc caatcagggg atcatggaat
     1021 ttgtcgagat gtttaaagca ccgataaaag tgctgcatcc cttgttaacc gccacccagg
     1081 aaggtaacta caacgggacg gaaggtatct ccgccctgcc gttcaacggg attattctcg
     1141 cccactcgaa cgaatccgaa tgggtcactt tccgtaataa caaaaacaac gaagccttcc
     1201 tcgaccgtgt ttacatcgtg aaggtgccgt attgcttgcg catttccgaa gagatcaaaa
     1261 tctacgagaa attgcttaat cacagtgaat tgactcacgc cccctgcgcc cctggcacgc
     1321 tggaaacgct gtcacgtttt tctattcttt cgcgtctgaa agagccagaa aactccagca
     1381 tttattcaaa gatgcgggtt tatgatggtg aaagtttgaa agacactgat cccaaagcca
     1441 agtcgtatca ggaatatcgt gactacgctg gtgtcgatga agggatgaac ggtctgtcga
     1501 cgcgttttgc gtttaagatc ctctcccgcg tgttcaactt cgatcatgta gaagtggcgg
     1561 caaacccggt ccatctgttc tacgtcctgg aacagcagat cgagcgcgag cagttcccgc
     1621 aagagcaggc agaacgctat ctggagttcc tgaaaggtta tctgatcccg aaatatgccg
     1681 agtttatcgg caaagagatc cagacggcct accttgaatc ctattcagaa tatgggcaga
     1741 acatttttga ccgttatgtt acctacgcgg atttctggat tcaggatcag gagtatcgcg
     1801 atccggatac cgggcagttg tttgaccgcg agtctcttaa cgccgagctg gagaaaatcg
     1861 agaaaccggc ggggatcagc aatccaaaag atttccgcaa cgagattgtt aacttcgtac
     1921 tgcgagccag agccaataac agcggacgta atccgaactg gaccagctat gaaaaactgc
     1981 gcacggttat tgagaagaaa atgttctcta ataccgagga gctgttgccg gttatttcgt
     2041 ttaacgccaa aacgtcaacc gacgagcaga agaaacacga cgactttgtc gaccgtatga
     2101 tggaaaaagg ctacacccgt aaacaggtgc gtttactgtg cgaatggtat ttgcgcgtac
     2161 gtaaatcgtc ttaacaaccc tggcccggta cacatgctac cgggcctaca acgacagcga
     2221 accgtgggcc agagaagcgg caacacaggc gtagcatacc gttggcaaat gtagtacggg
     2281 gggcatatga cctggtttat tgaccggcgt ctgaacggca aaaataaaag catggtgaat
     2341 cgccagcgtt tcttacgccg ttataaagcg caaattaaac agtcgatctc cgaggccata
     2401 aataagcgtt cggtgactga cgtcgacagc ggcgagtccg tatccattcc cacggaagat
     2461 attagcgaac cgatgtttca tcaggggcgt ggtggcctgc gccaccgcgt acatccgggc
     2521 aatgaccatt tcgtccagaa cgaccgaatt gaacgtcctc aggatggtgg cggaggttcc
     2581 ggcagtggtc agggccaggc cagccaggat ggtgaaggtc aggatgaatt tgtctttcag
     2641 atttcgaaag atgagtatct tgatctgctc tttgaagatt tggccttacc aaatctgaaa
     2701 caaaaccaac agcgccagtt aaccgaatat aaaacgcatc gggcaggtta taccgctaac
     2761 ggcgttccag ccaatatcag cgttgtacgt tcattgcaga actcactggc gcggcgcaca
     2821 gccatgacgg cgggcaagcg gcgggaactt catgcactgg aagagaattt ggccatcatc
     2881 agcaacagtg aacctgcgca actgctggaa gaggaacggc tgcgcaaaga gattgctgaa
     2941 ttgcgcgcca aaattgaacg cgttcctttc attgacactt tcgatttacg ttacaagaac
     3001 tacgaaaaac ggcccgatcc ctccagccag gcagtgatgt tttgcctgat ggacgtttcc
     3061 ggttcaatgg atcagtccac taaagatatg gctaaacgtt tttatattct gctgtatctg
     3121 ttcctcagca gaacgtataa gaacgtggaa gtcgtataca tccgccatca tacccaggcg
     3181 aaagaagtcg atgaacatga gtttttctac tcgcaggaaa ctggcggcac cattgtttcc
     3241 agcgccctaa aactgatgga tgaggtagtg aaagagcgtt ataacccggc acagtggaat
     3301 atttacgcag cccaagcatc agacggcgat aactgggccg atgactctcc gctttgccat
     3361 gaaattctgg cgaaaaaaat attacccgtt gttcgttatt acagctatat cgaaattaca
     3421 cgtcgcgcgc atcagacact gtggcgagaa tatgagcatc tgcaatctac tttcgacaac
     3481 tttgcgatgc agcatatccg cgaccaggat gatatttatc cggtgttccg tgaactgttt
     3541 cataaacaaa atgcaacagc taaagactaa aactgtcagc cgggtcatta tcgcctggct
     3601 g
//
LOCUS       DAZFRE010000216         3404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFRE010000216 DAZFRE010000000
VERSION     DAZFRE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.221"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..453)
                     /locus_tag="ACHKUI_005042"
     CDS             complement(<1..453)
                     /locus_tag="ACHKUI_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6601044.1"
                     /translation="MYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVLSPENITFTRWLKHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVL"
     gene            complement(872..2092)
                     /locus_tag="ACHKUI_005043"
     CDS             complement(872..2092)
                     /locus_tag="ACHKUI_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HFO6601045.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISRAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
     gene            complement(2111..2629)
                     /locus_tag="ACHKUI_005044"
     CDS             complement(2111..2629)
                     /locus_tag="ACHKUI_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFO6601046.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKG
                     TEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSEL
                     SYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRL
                     RKWAKNNNISLK"
     gene            2764..3012
                     /locus_tag="ACHKUI_005045"
     CDS             2764..3012
                     /locus_tag="ACHKUI_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6601047.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKGKERIQSLLEEIWQDDSWLPAA"
     gene            3009..>3404
                     /gene="umuD"
                     /locus_tag="ACHKUI_005046"
     CDS             3009..>3404
                     /gene="umuD"
                     /locus_tag="ACHKUI_005046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6601048.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRTSGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFG"
BASE COUNT          856 a          897 c          795 g          856 t
ORIGIN      
        1 cagtacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccagatgtc
       61 acaccagtca cctctttttg cggtgaaatg cacagtgata gtttccctga catcatccgg
      121 taacccttcc cagcgacgct gtccagtacc gctctgcagc cagagttcat gcagcatcag
      181 gcagttttgc tcatccagca gtacaccgtc ctgcaggtgc ttcagccagc gggtgaacgt
      241 gatattctcc ggcgataaaa caccacgtcc ggcagccgtc agtccggggc atggttcaaa
      301 ccgcacatct tcagtggtct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
      361 ttcatttgtg gcgcggcgat aaaacggtgt gactgcaccg ctggccagcc gtttaatctc
      421 agcaatcttt gccggttcac cagaaaaata catacgattc gagcaccagt ttggcatggg
      481 aattctcctc tcacatacgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
      541 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaacga cgaaacgtga
      601 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg attaacgtgc
      661 cggcacggcc tgggtaacca agtattttgt ccacagaacc gtgcacaaaa tgctgtggat
      721 aagctggaca cagcagtcca cagcaggcat acagccacac cctcagagaa ttgagcgttt
      781 tttagtcaca ataatgacat ttgactgtgt gtggattcag ttattagaat actgtatata
      841 catgggtctt ccccgatcat ggtgggaaga ctcagtacgc catattcagt ttcccatagt
      901 ggaacataac tcccagttta aagcgttccc ggtttcggta tcccctggcc tttatcctca
      961 gcagtctgat cttgctgttc agcgcctccg catttccatt cgagacgcga tgacgcattg
     1021 cattcagtat tccgtataac cgttttttaa tggttttcgc tgcgtttttc atcatcggca
     1081 catcacattc agacgcccgc gatatccact gcaaccagtc attcctgcgt tctgtgctcc
     1141 aggggcgatc ccagatattt tttgccagct ctttcagtgt ccagcactgg cttgtctgtt
     1201 gcatctgttc ccgcaaccac atcagctttt cctgccggga ctcagtcatc catttgtcgc
     1261 tgtactgcca caggaagcgg gtacctttgg cctgacgacg actttcaaca gggaggtgcg
     1321 gatgttcatt ctggcgggtt ttatcaacca cttcgcccag ttgcttcgcc acatggaagc
     1381 gatcgaaggc gattttctcg accgcattgg gtaaatggat acgcgctgct cttatatagc
     1441 ccgcattcat gtccatcgac agtgttttga tggccaacaa ctgactgtca gtaagcgaac
     1501 ggagatagct ggcaagactc tctgtgccac gatcatcggt taatgccagt gcccgcccgt
     1561 cgcgatcaga taccacggtt atatacctat gccctttttt aaaggcgact tcgtctacat
     1621 tcatatgacg cactgataaa ggctttttaa tccgcgacag gcctcgctta accgcgcggg
     1681 tcatgatgcc gtcaacggca ttccagctaa gtttaagttg ttttctgacc gcatcaacgg
     1741 tgctgatttt aagccatgag agcacgaacg attcgaacag cagagtgtac cgactgccgg
     1801 aacctgccca cggtacaggc agagtctggc agccatgctc cgggcacata acgcggggaa
     1861 catcggcttc tactaatgtc atgaactggc aggtatcaag gtggcgccat ttacgatgtc
     1921 ggtgatcgtg aacagaacag gatttcctgc aggtcggaca ggttaactga gtattttcag
     1981 caattccgac tgtaacagta acggaacctg cattttcatc gagggtaagg gatttaacct
     2041 gccatggtgc agtcaggtta aggatatggg catagaggga tttttcgtcc atataatttc
     2101 attctgaagc ttactttaaa ctgatattat tattttttgc ccactttctt aatcttccat
     2161 tagcgttggc gtaaggcgaa gatgtgttaa atgagatcat tctccccatc gtccattttg
     2221 tgtaccatgg tcttccatac aaaatatcat cggtacgatc attaataagg ttcacaatct
     2281 cattttttac agtctgaagt tcagctacta acaactcata acttaactca ctgtaatctg
     2341 agtaaaattt ttgagcaaga aggccaagct gattccattt atagccagtt tccggaaaat
     2401 cgacaggcag acctttagca tcagaagcga tccactttac aacaagagca ttccatccaa
     2461 gcagatacga aacgagatca cgaacactca tctccgttcc tttggcgtgt ccgtccattg
     2521 atttatctga agtaatttct ggtgggattg tgttgaggta actaattaat ttactaaaat
     2581 ttttatcaat agctaaaagc agttcagctt ttgtttgcgg cacactcata agaattccct
     2641 ctcatgtaaa aagagacacc ataaaggtac tgtgtggtgt ttgaaaatca ctcaattatg
     2701 taccacccac cacaacaggg gaagacccat atacatacag caaaagaaaa gggagatgag
     2761 aacatgatcc gcattgaaat tcttttcgac cgccagagca caaaaaacct caaatcaggc
     2821 acacttcagg cgctacagaa tgaaatcgaa caacgtctga aaccacacta cccggaaatc
     2881 tggctgcgta tagaccaggg cagcgccccg tctgtatctg tcactggagc ccgcaacgac
     2941 aagggtaaag aacgtatcca gtctctgctg gaagaaatct ggcaggacga cagctggctg
     3001 cccgcagcat gagtaccgtt tatcaccgtc cggctgaccc ttcaggcgat gacagttacg
     3061 tgcgaccgtt gtttgccgat cgttgccagg ccggttttcc ttcgcctgcc actgattatg
     3121 ctgagcagga actggatctg aacagctatt gcatcagcag acctgcagcc accttctttc
     3181 tgcgcaccag cggtgaatcg atgaaccagg ctggcgtgca gaatggtgat ctgctggtag
     3241 tggacagggc cgaaaaacca caacacgggg acatcgttat cgctgagatc gacggtgagt
     3301 tcaccgtcaa acgactgctg ttgcgcccac gcccggcact ggagccggtt tcagacagcc
     3361 cggagttccg cacactgtat ccggaaaaca tctgtatttt tggt
//
LOCUS       DAZFRE010000217         3235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFRE010000217 DAZFRE010000000
VERSION     DAZFRE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.222"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            21..896
                     /locus_tag="ACHKUI_005047"
     CDS             21..896
                     /locus_tag="ACHKUI_005047"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6601049.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            945..2417
                     /locus_tag="ACHKUI_005048"
     CDS             945..2417
                     /locus_tag="ACHKUI_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6601050.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            2421..>3235
                     /locus_tag="ACHKUI_005049"
     CDS             2421..>3235
                     /locus_tag="ACHKUI_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6601051.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLA"
BASE COUNT          846 a          515 c          817 g         1057 t
ORIGIN      
        1 tgttatatag gtgaaaatga atgataacac tggctgttga tataggagga acgaaaattt
       61 cagcggcgtt gatatctgat gacggttcat ttttgctgaa aaaacagata tcaacacctc
      121 atgaaagatg tcctgacgaa atgacagggg ctttacgtct actggtttct gaaatgaagg
      181 gaacagcgga gcgatttgca gtagcatcaa cagggattat taataatgga gtattgacag
      241 ctctgaatcc agacaatctt ggagggctga aagagtatcc tctgaaaaat ataatggaag
      301 atataactgg tttaaatgga agtgtgataa atgatgccca ggcagccgca tgggcagaat
      361 atacagtttt gccgaaagaa atttgtgata tggtttttat aaccgtttct acgggggttg
      421 gagggggaat tgtagttaac agaaaattat taacaggtgt ttctggatta gccggacatg
      481 ttgggcatat attgtccgga gtaacggata cagaatgtgg atgtggtcgt cggggatgcg
      541 ttgaagcggt ttcatctggt cgggccatca tgggggcagc aaaaaataaa cttgcaggat
      601 atagcaccaa atatattttt gaattagcga gacagggata taaggaagca gaatttttga
      661 cagagcgttc tgccagtact attgctgaat taatagtaag tttaaaatta ctgcttgatt
      721 gtcaggttgt tgttgtcgga ggaagtgttg gacttgctga tgggtatgtt cagaaagtgt
      781 cgaaacatct ttccatatat tctgaaattt gtaatgtaat gctgtttccg gcatatttca
      841 gaagtgactc tggtcttatt ggtgcaactc tttgggaccg ggattgtatt acttaaagta
      901 atactccagt gtattataaa tccgctatta ttgtgaggtt taatatggac tcctgtattc
      961 agaaaaagca ggtttggtat aagcatcttt cacccagaca gtggaagata ttcggtgcgg
     1021 catggacagg ttatttactt gatggttttg atttcgttct gatatctctg gttcttacag
     1081 aggttcagca tgaattcggc ttgacgacaa tagaagcggc aagccttatt tccgctgctt
     1141 ttatttctcg atggtttggg gggcttgcta ttggcgcttt atcggacaag atggggcgca
     1201 ggatggcaat ggttttaagt atagtgctat tctctctcgg aacccttgcc tgtggtctgg
     1261 ctcctggata tgcagtgatg tttattgctc gcattgttat tgggcttggt atggcaggtg
     1321 aatatggctc aagtgtaaca tatgttatcg aaagctggcc tgttcatctc agaaacaagg
     1381 caagtggttt tttaatttct ggattttcta ttgggggagg tttagctgca caggtttata
     1441 gcattgtagt accactatgg ggctggcgtt ctcttttctt tgtcggcatg cttccaatat
     1501 tatttgcttt ttatcttcgc aagaatcttc cggagtctga tgactggcaa aaaaggcaac
     1561 aagagaataa acccgtcaga acaatggtgg atattctgta cagagaaaag aataaataca
     1621 taaatattct tctgtcttgt attgcatttg catgtctata tgtttgtttt tcaggcgtca
     1681 ctgcaaacgc tgctttaatc acagttatgg cattgtgctg tgcggctgtt tttatcagtt
     1741 ttatatatca gggtatgggg aaaagatggc caacaggcat tatgttgatg cttgtggtta
     1801 tgttctgttt cctctacgga tggccactgc aggcttttct tcctacatgg cttaaggttg
     1861 atatgcagta ttcaccagaa actgttgctc tcatatttat gctggcaggt tttggctctg
     1921 cagctggaag ttgtataggt ggttttatgg gggactggct tggaacccga aaagcatatg
     1981 ttatatcact tcttatcggt cagcttgtta ttatccccgt ttttcttgtg gaccgggact
     2041 atgtatggtt acttggcctt ctaatcttta ctcagcaggt ttttggtcag gggattggtg
     2101 ctctggtgcc gaaaataatc agtggttatt ttaatgtgga gcagcgtgcg gcaggactgg
     2161 ggtttattta taatgtaggt tcacttggtg gagcatgtgc gcctattctt ggtgcagttg
     2221 ttgcgtctca tacaagtctt ggtacagcaa tgtgttctct tgcttttatc ttaacatttg
     2281 ttgttcttgt ccttattggt tttgatatgc catcacgtgt acagagatgg atccatccag
     2341 aagctgcgct tgaatatgat actgtcgatg gaaaaccttt ttatggagca agaaaaaaga
     2401 atgttgcaga agaataacgg atgctatcag ggaataaaaa aatgattcat tacagggaga
     2461 atatatattc tcttgataat tatgagcgtt acataactga cagttcgcta tttgagaata
     2521 ctggcgatgg cataatatat acaggtattg tgccccttat tacagttgtt gagcctgttg
     2581 tatctaatca acatagtgtg ctgattatac ctggaggtgg atacaggagg attgtttatg
     2641 aaagagaggg acttgatgtt ggcagatgga tagcaggtca gggatatacg gcatttattc
     2701 tttcttacag aatgccctcc gttaatggtt attattcctt tgcagatgcg aagaatgccc
     2761 tgaactttat ttcttactgg gccggaatgt cggagagaac atttaagagt gttggatgta
     2821 tggggttttc ggcaggtggt catcttgctg gatatattac tcatctccat tatgaggaag
     2881 agacttctgt gcgtcctgac tggatggctc tgatatatcc agttatatct cttgacagga
     2941 gtattgctca tcagggatgc cggaaacgtc ttctggatgc aggtgaaagt gaatattcag
     3001 ccagtcttga aaatagtgtt acagagtata tgccaccagt ctttattgtg catgcaacag
     3061 atgataagga tgttccagtt attcattctc tgagatattt taactctatt cgggataagg
     3121 gacatcatgc agaagctcat ttttttgata agggaggtca tggatttggt gtatccggaa
     3181 aaaatggata tccgatagaa aaatggcaaa gcctgcttct ggaatggctt gcgga
//
LOCUS       DAZFRE010000218         3226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFRE010000218 DAZFRE010000000
VERSION     DAZFRE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.223"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(60..857)
                     /gene="istB"
                     /locus_tag="ACHKUI_005050"
     CDS             complement(60..857)
                     /gene="istB"
                     /locus_tag="ACHKUI_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFO6601052.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
     gene            complement(857..2029)
                     /gene="istA"
                     /locus_tag="ACHKUI_005051"
     CDS             complement(857..2029)
                     /gene="istA"
                     /locus_tag="ACHKUI_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFO6601053.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSRDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            2133..2273
                     /locus_tag="ACHKUI_005052"
                     /pseudo
     CDS             2133..2273
                     /locus_tag="ACHKUI_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2270..2581
                     /locus_tag="ACHKUI_005053"
     CDS             2270..2581
                     /locus_tag="ACHKUI_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601054.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            2657..>3226
                     /locus_tag="ACHKUI_005054"
     CDS             2657..>3226
                     /locus_tag="ACHKUI_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6601055.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRVDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIG"
BASE COUNT          659 a          926 c          840 g          801 t
ORIGIN      
        1 gtcaacgcca cgatgtttga ccgttatttg ccattttcat gcccgctaag atactttcgt
       61 tactgatgct gtccgctttt caccatttca tcatcactga ttggcgttgt gtttttggtc
      121 agcactccag ctttacgttt ctcttttaac cggtaactct ctcctttgat attcagcgtg
      181 gttgagtgat gtagcaaccg atccagtatc gctgttgcca gcacgtgatc tccgaacatt
      241 tctccccagt ctgcgaaccc tttgtttgac gtcagtatga tgctcgcttt ttcatatcga
      301 cggttcagta gccggaagaa caggctggct tcctctctgt tcatcggcag atagcctatt
      361 tcatccagga tcaacacccg ggcataactc agttgctgca gctgacgctc cagccggttt
      421 tcctgtttcg ctttcatcag tgtcgcgatc agtctgtcca gtggcataaa cagtacccga
      481 tgtcccgcat ccaccgcttt cacgccaaga gctatggcca gatgagtttt tccgacacca
      541 ggaggtccca gcaggatcac gttttcgctg cgctccacga acgccagacc agccagttcc
      601 cggacaacct tacggtcgat gcccggctgg aaggtaaagt cgaactgctc cagcgttttg
      661 acccacggca gacgagcctg cttcagcctg gactccatac cgcgctgatg cctgccgttc
      721 cattcctgct gtagcgccat gcacaggaac tcccggtagt tcagctcttt tttagctgcc
      781 tgttccagca ggctttcaac gtgataactc agatgctcca ttttcaggcg actcagcagg
      841 acttccagct catgcatcac aacagctcct cataggcact cagtggtcga tgttccacct
      901 gactgacctg ctgccagagc ggggcgtgat gctccggcac tgtctgccag ccagacgatg
      961 ccgaacagag gcgatgtgag gccaccagtt tctcattact gtagatccgc aactcgtcat
     1021 ccagcgatat tcgtatcgat accggctgac cacacagcgc ttcgggaacg ctgtaacgat
     1081 taccaccaac ctcgatatag ctgtcccggg acacatggcg gatgtcgaag taactggtat
     1141 cgaagtccgt atccggtaac ggctgcagat gttcctgctc cagcgcgaag cgctgttccg
     1201 gcgtttcttt gaactggcga agttcccgtt tgtcagccac atcggctatc cattgctcca
     1261 gttgttgatt aacatgagtg aagctgtcga acctgcggta ccgaacgaag aagttctcct
     1321 tgaggtattt caccatccgc tcaaccttac cttttgttct ggccctgcgt ggacggcatg
     1381 cccgtggcag gaagttatag tggtcggcca gcaacaggaa tccggagttg aacacgactt
     1441 tcccgttgtt attcttcagc accgcagcct tctggttatc aaccagcacc gttttcacac
     1501 aaccaccgaa gtagcggaag gcgcgaacca gtgattcgta ggtatgctca gcatcctgtt
     1561 ttggtgcggc gaagacatgg aagcggcggg agaaccccag cgtattaacc gcaaagttaa
     1621 ctttgcaccg ttgcccggca acctccacct caacttcgcc ccagtcgtgc tggagctggt
     1681 atccaggctg agtttcgaag cgaactgttc tttttgacgg acgcatttta cgtttgggct
     1741 ggatgtagta acgcagcatg gaacgtccgc ccgtataacc cattgcctta atctccgcaa
     1801 agataacctc actattccag acattctctg ccaggcgcat gtcgatgtaa tccataaacg
     1861 gtttcagctt aaccattttg tggcgggtct ttctggctgg cggttcaggg tatttgaggt
     1921 agcgtctgac cgtccgttca gagcaaccaa tctgagtcgc aatatcgaca atgtacgcgc
     1981 cctgctggcg catttgcttt atcatgtaaa agtcctctct gctcagcatg ttgatgtcct
     2041 ttctggtgtg agaaccttaa ggaaacaaca tgttgggtgg agcggacaat ccaaatggtg
     2101 aattaccgtc ttatatcact ggcgctgaca gctggcagtt gtatgagcgc ggagagcttg
     2161 ttgatgtgct gtggggggaa ctggaatggt cttccccgac agatgacgat gagctgccgg
     2221 aagtcaccgg acctgcgtgg atagtcgaca atgtggcgca ttatggcgga tgaagtatga
     2281 cggagacggg cgggcaaccg cccgtttctt ttccggtaaa ggatgtcgcc gtgctcctct
     2341 ttttactgcg ccggctgacg cggcgcggca taaacgctgc ctgtggtcag tgtcccgcgt
     2401 ccggtgggca cgggacgggc gcttttaccg ctcccggctg gcgtcggtga cagtgtacgc
     2461 cagcctgtcg cccttttctg atgaacgccc ttcaagccgc tttcgcggca tagccttgcc
     2521 gtcagaaaga cgacggctgc ggtattccac ggtcggcctg acccgttacc ggacgcggtg
     2581 aacagcccac aggcagcggg gaacgggcac cacaggggtg ccctcccggt gccctctgta
     2641 agagaaggag ttttgtatgt cccgttttgt cctcggtaac tgcatcgatg ttatggcccg
     2701 tattcctgat aacgccattg atttcatact gactgacccg ccgtacctcg tcggtttccg
     2761 tgaccgttcc gggcgcacca ttgccggtga tgttaatgat gactggctgc aacctgccag
     2821 caatgagatg taccgcgtac tcaaaaaaga cgcgctgatg gtcagcttct acggctggaa
     2881 ccgtgtcgat cgctttatgg ccgcctggaa acgcgccgga ttcagcgtgg cagggcacct
     2941 cgtgttcacc aaaaactaca catccaaatc tgcgtatgtc gcgtaccgtc acgaatgcgc
     3001 ttacatcctg gcaaagggca gtccggcccg gccccgtaac cctctgccgg atgtgctggg
     3061 ctggaaatat tcaggcaatc gtcatcaccc gacggaaaag cccgttacca gcctgcaacc
     3121 actgattgag agcttcacac atccgaacgc gattgtgctg gacccgtttg caggcagcgg
     3181 ctcaacctgc gtcgctgccc tccagtccgg acgccggtat atcggt
//
LOCUS       DAZFRE010000219         3224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFRE010000219 DAZFRE010000000
VERSION     DAZFRE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.224"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            77..298
                     /locus_tag="ACHKUI_005055"
     CDS             77..298
                     /locus_tag="ACHKUI_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6601056.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            461..829
                     /locus_tag="ACHKUI_005056"
     CDS             461..829
                     /locus_tag="ACHKUI_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6601057.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            919..1293
                     /locus_tag="ACHKUI_005057"
     CDS             919..1293
                     /locus_tag="ACHKUI_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6601058.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            1290..1778
                     /locus_tag="ACHKUI_005058"
     CDS             1290..1778
                     /locus_tag="ACHKUI_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6601059.1"
                     /translation="MKRSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            1790..1987
                     /locus_tag="ACHKUI_005059"
     CDS             1790..1987
                     /locus_tag="ACHKUI_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6601060.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            2084..2653
                     /locus_tag="ACHKUI_005060"
     CDS             2084..2653
                     /locus_tag="ACHKUI_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6601061.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRYRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            3031..3142
                     /locus_tag="ACHKUI_005061"
                     /pseudo
     CDS             3031..3142
                     /locus_tag="ACHKUI_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          859 a          850 c          740 g          775 t
ORIGIN      
        1 atggtctgct ttaacccgtc acaaccacat cacgcttgtt ttcactttta tcttctgtct
       61 tcagaggtat cacactatga aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca
      121 acatccggca tcccgtcttt ttcctttctg taccggcaaa taccgctggc acggcagcac
      181 tgaagcgtat accggtcgtg aagtgcagga tattcccggt gtgctggccg tgtttgctga
      241 acgccataag gacagttttg gcccgtatgt ccggctgatg agcgtcaccc tgaactgatg
      301 ttccgtcacc cggaaaacga gtacgaccag atggactcag acgccgctgt ggatgaggcc
      361 attctcgtca atgcatacat cttcaccgaa accggtcagc gcgccggctg acctgtcatc
      421 gttaaaccat tcttaaccac ttttcagaga ggattttatc gtgtcagaca aactctccgg
      481 gataactcat cccgacgaca atcacgaccg cccctggtgg gggcttccct gcacagtgag
      541 gccctgtttt ggtgcccgtc tggtgcagga gggtaaccgc ctgcattacc ttgccgaccg
      601 cgccggtatc agaggccgct tcagcgacgc agatgcatac catctggacc aggcctttcc
      661 gctgctgatg aaacaactgg agctcatgct caccagcggt gaactgaatc cccgccatca
      721 gcataccgtc acgctgtatg cgaaaggtct gacctgcaaa gctgacaccc tcggtagcgg
      781 tggttacgtt tatctggctg tttatccgac gcccgaaacg aaaaagtaaa ccaccggaat
      841 aaccttctgc tccggcctgg tgctttcacc acgccacttt tccatttttc atctctgcat
      901 atcaggaaaa ccttcagtat gaaaacatta cccgatacac acgtacggga ggcatcgagc
      961 tgcccgtctc ccgtcaccat ctggcagact ctgctctccc gactactggg ccagcattac
     1021 ggccttacac tgaatgacac accgttcgcc gatgaacgtg tgattgagca gcatattgaa
     1081 gctggcattt cactgtgtga tgcggtgaat tttctcgtgg aaaaatacgc gctggtgcgt
     1141 accgaccagc cgggattcag cacttgtccc cgctctcagt taataaacag tattgatatc
     1201 ctccgggctc gccgggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac
     1261 attaccctgg gtaagcatcc ggagaaacga tgaaacgttc cctgatgctg gaagccgaca
     1321 gaattaatgt gcaggcactg aacatggggc gaattgtcgt tgacgtcgat ggtgttaatc
     1381 tcactgaact gattaacaag gtcgctgaaa acggttattc actccgcgtg gtggaggaat
     1441 ccgaccaaca gtcaacctgc acactaccac cgtttgcaac ccttgccggc atacgctgca
     1501 gtaccgcaca tatcacggaa aaggataacg cctggctgta ctcgctgtca caccagacca
     1561 gtgacttcgg tgaatcagaa tggattcatt tcacaggtag cggatatctg ttacgtaccg
     1621 atgcgtggtc atatccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc
     1681 gtctggttat cacacttacc cgacgttatg gcgtcagtct cattcatctg gatgccagcg
     1741 ctgaatgcct gccgggttta cccactttca actggtaacc aggaacaaca tgaaatcatt
     1801 aaccacggaa accgcactgg atattctgat tgcgtggctg caggacaata tcgactgcga
     1861 atcgggaatt atctttgaca acaatgagga taaaacggat tcagcagcac tgttgccctg
     1921 tatcgaacag gccagagagg atatccgtac cctgcgccaa ctgcagcttc agcaccagaa
     1981 ccggtgagtc tcactcatca tctcactcac cagacttcat tccactgacg ccagcctgaa
     2041 cacggctggc gttttcattt atctgcaaaa aggaatatcg attatgtctg aaatcacagt
     2101 ctcccgtccg gaagtggtca acgagaatac ggacgttatc tgctccacct cagtcaggta
     2161 caggtcactg gaatatgata attttccgga aatcagcgaa gcgaacattc tgagcacatt
     2221 tgaacaactg caccagaaca aagatgaagt gtttgaacgg ggagtgatca acgtcttcaa
     2281 agggctgagc tgggattaca aaaccaactc accctgtaaa tttggcagta aaattatcgt
     2341 caacaatctg gtgagatggg accagtgggg atttcatctt atcagtggaa tgcaggcaga
     2401 tcgcctggct gacctggaaa gaatgttgca tctgctcagc ggtaaaccga tccccgacaa
     2461 ccgagggaat atcaccatta atctggatga ccacatacag tccgttcagg gtaaaggacg
     2521 ctatgaagat gagatgttca tcattaaata ctttaagaag ggatctgcac acatcacttt
     2581 caaaaggctg gagctgattg acagaattaa cgatataata gccaggcact ttccttctgt
     2641 gctctcagcc tgaccccgag tttgattccc tttcgatatc aaaagggact gcgggtacaa
     2701 aagagggtac atctttcacc aaaccaaaca aaataaacta atatcaacat gatagaagca
     2761 ttcttcgatt ccgagtccgg caccaaattc atataaacgg acctccacgg aggtccgttt
     2821 ttcgtttcag gacgccacga tttaagcgtc ctgccgccaa atcaattcta ccgaactcaa
     2881 ccagattctc cccacatcac cagcaatttg cgggcatatc ccaattcggg aaaatttgtt
     2941 tctgagctat agcgctgact gacgtgaaat gtcgtgcggc cccgtgatgc tgttgaacgt
     3001 caaatgacgt catcaggagc gtaacgcacc cataaagcac aacatcgggc agaacgccaa
     3061 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgtttg ctcatgcaaa
     3121 gactaacaat ccattaaaat agtaagcgct ccggacaatt tttcatggat tattttctga
     3181 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaaa
//
LOCUS       DAZFRE010000220         3182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFRE010000220 DAZFRE010000000
VERSION     DAZFRE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.225"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..595)
                     /gene="yeeO"
                     /locus_tag="ACHKUI_005062"
     CDS             complement(<1..595)
                     /gene="yeeO"
                     /locus_tag="ACHKUI_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6601062.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLING"
     gene            756..831
                     /locus_tag="ACHKUI_005063"
     tRNA            756..831
                     /locus_tag="ACHKUI_005063"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Asn,seq:gtt)
     gene            complement(869..1819)
                     /gene="cbl"
                     /locus_tag="ACHKUI_005064"
     CDS             complement(869..1819)
                     /gene="cbl"
                     /locus_tag="ACHKUI_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO6601063.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1921..2838)
                     /gene="nac"
                     /locus_tag="ACHKUI_005065"
     CDS             complement(1921..2838)
                     /gene="nac"
                     /locus_tag="ACHKUI_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6601064.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPRTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLREDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT          876 a          814 c          700 g          792 t
ORIGIN      
        1 taccgttaat cagtagcggt attttcgtat tccctgcacc acgaagtgcc ccgctaccaa
       61 taagagtgat ggcagctgct ggataactga gtacggtcag ctccagataa gtcaacgcca
      121 gtgctttaac gtctgtcgtg gcatcacccg cgacgaaatc aataatttgt tcgccaaaat
      181 gatgaattag cgttgccaac agtacggcaa acaacgtcat gatcaccaat gactgccgcg
      241 tcgccaccct cgctcgtcgt cgatctcgct taccgagact aaatgctaca acgacagtag
      301 taccaagatc gatagctgca aaaaaagcca taatgaccat attgaagctg tccgccaacc
      361 ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc aggaccccca
      421 tcaacaggac acaggcgttc tccatgaaga taggaacagc aagcggggtt atctcgcgcc
      481 agaacaacac tttgtagctc ttgcgtttag agtgccagcg agtgccgtga acaacctggc
      541 gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac cacatttcaa
      601 atgatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc
      661 aacaaactgt tgatagaaac ggcaaacagt tgggaaattt aaaaatcggg tttgacaaaa
      721 tatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag tcggtagaac
      781 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaatttctga
      841 aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc tcttcactgt
      901 tttccatcac ctggcgcttg atatcctcaa ctgacagtcc tgcattacaa agttccagaa
      961 agcgccagac atagttacgc tgaagttgcc ctcgcttcag tcccaaccag acagtattag
     1021 catcgaaaag atgccgcgta tccaggcgga ttaaattctt ttcctcttgt tcgccgctgg
     1081 attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta atgacatcag
     1141 aatcctgtgc acttaataca atatctgccg gcaaaccttt gcgggcaaat gcgtcatcaa
     1201 tgcgtgagcg ccccgtaatc ccctgtcggt aagtaattaa cggccacttc gctattgatt
     1261 ccagcgtcaa cggtgtaatt tgcgtcaagg gatgatcgag tggaacaagc aaactatggt
     1321 gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc tcgctggcga
     1381 taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggt gtaccctgga
     1441 ttagctccag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc tcaggcaagc
     1501 tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta tcgttggtaa
     1561 acaggtctgc gagccgacga acattactgg cttcattcag aatgcgttct gcaatgacca
     1621 gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga acaaatattt
     1681 cgatgccaag ttcatcctcc agttcccgaa tatgacggct aacgcctgac tgtgaggtaa
     1741 aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg cggattatct
     1801 ttagttgttg gaaattcacg gtaaactccg ggcagttcag atatcccgtt attgttaaag
     1861 tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc gatataacgc
     1921 ttagctcacc aattgccact gccttttttc catcactgga gaactaatca ctgacattaa
     1981 caactctttc actgcctgtg cctgtggtga caagtttgct ctggcgggta aatttaagga
     2041 caaagagaga ctcattgaag gagtggtaat gcgtgacatc cacccattta ctgcgccaca
     2101 taacgaacgc gcagccgatt caggtaatac tgcaacgccc atgccgctgg caatcgctgc
     2161 ggtaagcgtg gcaatagact caatttcacc aatgactttt gccgtgagcc gccgtaggga
     2221 aaaagcctca tcaacacgaa gtctaacagc gctgtaatca ctggggagaa aaaggttcat
     2281 ttgcgcaata gcattaacat caacgctttg ccccgggcaa tcttgagttc ctaccaggaa
     2341 aagatcttct ctcagcaaag cctgactgga taccccagcc acaggggaat gctcataaat
     2401 caccgccata tcgagttggt gatttatcag tttttcgtta agcactgcac cactattttc
     2461 atgaagatag ataacgacct ccgggaattc agcgcgaacc gcctgtaata agggcatggt
     2521 gatggatgac gcagcggttc ttggcgcaaa gccaatcgag acttgccccg ataatgactg
     2581 accaacgtta tgcaccgcca gttgggcctg ctcacactga cgtaaaatgg cccgtgcatg
     2641 ggtatagaga atttttccgg cgcctgttgg cgtaacgccc cgttttgtgc gaatcaaaag
     2701 ctgttgattt aactcacctt ccagtgtggc aacctgctgg ctgagcgctg gttgtgcaat
     2761 atgcaatact tcagcagcct gggtcaggct accaatatct acaattttta cgaagtattt
     2821 caggcgtctg aagttcatgt tgcctccggt ttttaagaat cggcccaagt gccgccatta
     2881 cttacaacca gaatgcaaga tgcttgccag ttctatttta gcgttgatgc gcaagctaac
     2941 caactgtcaa ataagagatt atgatagatt cgtcattggc cctttttatc agcagtcgca
     3001 ttcacctgcc ctataaaagg ggggtatgca ccacgatggt tcattaccca ataagattga
     3061 aagctcacca ctttgttgaa attgtcagca aacaaacaaa aaaatgcatt tcaccctttg
     3121 acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct gtagttcagt
     3181 cg
//
LOCUS       DAZFRE010000221         3113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFRE010000221 DAZFRE010000000
VERSION     DAZFRE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.226"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            143..1354
                     /gene="bluF"
                     /locus_tag="ACHKUI_005066"
     CDS             143..1354
                     /gene="bluF"
                     /locus_tag="ACHKUI_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO6601065.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            1559..2290
                     /gene="bluR"
                     /locus_tag="ACHKUI_005067"
     CDS             1559..2290
                     /gene="bluR"
                     /locus_tag="ACHKUI_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO6601066.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2511..2915
                     /gene="ycgX"
                     /locus_tag="ACHKUI_005068"
     CDS             2511..2915
                     /gene="ycgX"
                     /locus_tag="ACHKUI_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO6601067.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2968..3093
                     /locus_tag="ACHKUI_005069"
     CDS             2968..3093
                     /locus_tag="ACHKUI_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601068.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
BASE COUNT          910 a          645 c          664 g          894 t
ORIGIN      
        1 gttttttcgc caaaccgtct tcaacctttt ccacttttta tcaatgtaaa gaaacggtta
       61 tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct
      121 cgcccattac cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga
      181 cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgcg
      241 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
      301 tccggaagaa caggttcaaa tgatatatcg ggccatatgc caggatccac ggcactataa
      361 tattgttgaa ctgttgtgcg attacgcgcc tgctcgccgt tttggcaaag caggaatgga
      421 attatttgat ttacgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
      481 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
      541 ccttgcaact gaacaatcaa cctatttcga gattcctgct gaagactcct ggctttttat
      601 cgctgacgga tctgataaag aacttgattc ttgtaccctt tcatcaaata taaacgacca
      661 ctttgccttt catcctattg tcgacccttt atcgcggcgg ataatcgctt ttgaagccat
      721 tgtgcaaaaa aatgaagaca gcccgtcagc tatagcggtt gggcagcgta aagacgagga
      781 aatctacaca gcggatctca aaagtaaggc gcttgcattt gcgatggcac acgcacttaa
      841 gctcggtgat aaaatgattt caatcaatct attacctatg accctggtta acgagcctga
      901 cgcagtctct tttttacttg atgaaataaa ggccaatgct ctggtgcctg aacaaatcat
      961 cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcggttaa
     1021 atcgctaaag gctgccggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg
     1081 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     1141 caatgttcat aaaagcggac cacggcaggc aattattcag gcgatcatga aatgctgtac
     1201 atcacttgaa attcaagtct ctgctatggg tgtggcaaca ccagaagagt ggatgtggct
     1261 tgaatctgca ggaattgaga tgtttcaggg agatctgttt tccaaagcta aattgaatgg
     1321 tatcccttca gttgcgtggc cggagaaaaa ataattttca gcacattatt tcgcatgatt
     1381 tctggaaatc atcgcgaaaa tgaatgcgtt atgttttaat ttaatccaca ttaaaaataa
     1441 tgtttcatcc aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt
     1501 acaaagtatt attgggtcgt gtagaggcga cggagattta tgaccgcaag gaggaattgt
     1561 ggcttattac agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg
     1621 ggcctggcaa cgccgctacg gtttgttaaa accacagcgc agtgaaggcg gacaccgact
     1681 ctttgatgaa gaagacatac aacgcatcga agagattaag cgttggataa gtaatggcgt
     1741 tcctgtaggc aaagttaaag cattactgga aactaccagc caggatacgc aagatgactg
     1801 gagccgcttg caagaagaga tgatgtcaat tcttcgtatg gctaatcctg ccaaactacg
     1861 cgcgagaatt atttcactgg gtcgagagta cccagtagat caattgatta atcatgttta
     1921 ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta tgagcagtat
     1981 gtttgacggc gcattaattg aatacgcagc ggcctcactt ttcgaaatgc gccgtaagcc
     2041 cggtaaagag gccattctga tggcgtggaa tgttgaagag agagcacgat tgtggctgga
     2101 agcatggcgt ttatcattgt caggatggca catttctgtt cttgctgatc ccattgaatc
     2161 gccgcgcccg gaactgttcc caacgcaaac attgattgtc tggacaggca tggcaccaac
     2221 gagaaggcaa aacgaacttt tacaacactg gggtgagcaa ggttataaag tcatctttca
     2281 tgccccctaa attcaccttt atcccgcccc ttagacagaa tgttataatc tgaacccaca
     2341 acagcgtttg tagcggagct ggtccgttgc aaaatgccca ccgggagaat tcaggtaatc
     2401 cccctttcac actttgtaca tatcacagcc aaaggacaga ccagattgaa gtggtaaaac
     2461 tgatcgtgca cactacgcag tctcacacct aacaagaggt tattatcctg atggatcagg
     2521 tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga gcaaaacttc tcctggtttt
     2581 attcagaatt aaaacatcac cgtattgcac attacattta ttatctggct acggataaca
     2641 tcagaattat tactcacgat gacacggttt tgttattaag aggaaccagg aacctgttaa
     2701 aagttagtac gaccaagaac ccagctaaaa taaaagaggc cgcattgctt catattcgcg
     2761 gaaaatctac atttcgggaa tactgttcaa cactggcagg cgctggcgtt ttccggtggg
     2821 ttactgatgt taatcataac aaacgcagtt actacgccat tgataatacg cttttatata
     2881 ttgaagatgt agaaaataat aaaccattaa tctagctaaa gttgggagct taaaaaatgc
     2941 ttcataattc agtaaggcat tagcataata gaaataaaag tgcagagact atccctatgg
     3001 atgattaata ctgtcttttt attgctcccc ataaataatc accagaccaa tacgatcaaa
     3061 ttgatatttg aaatgtgttc acttgacttt tgatacgtta ttttataacg gtt
//
LOCUS       DAZFRE010000222         3032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFRE010000222 DAZFRE010000000
VERSION     DAZFRE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.227"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..426
                     /gene="traX"
                     /locus_tag="ACHKUI_005070"
     CDS             <1..426
                     /gene="traX"
                     /locus_tag="ACHKUI_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO6601069.1"
                     /translation="GFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILMMALWGPL
                     SGTSYGIAGLLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMT
                     VLTVGLVSCAGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            481..1038
                     /locus_tag="ACHKUI_005071"
     CDS             481..1038
                     /locus_tag="ACHKUI_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6601070.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            1190..1393
                     /locus_tag="ACHKUI_005072"
     CDS             1190..1393
                     /locus_tag="ACHKUI_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601071.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            2135..2596
                     /locus_tag="ACHKUI_005073"
     CDS             2135..2596
                     /locus_tag="ACHKUI_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6601072.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRILDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     HFMVRAGAAWVYEQYNTDPVLPVLQGEARQQKRGLWADSAPVPPWVWRSRK"
     gene            2699..>3032
                     /locus_tag="ACHKUI_005074"
     CDS             2699..>3032
                     /locus_tag="ACHKUI_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601073.1"
                     /translation="MNYQTVSELITSSNHNVLIVWDSASEVDGFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFYQGWPDLNNESAVMQFARVARKSGKQLIVAVREQDMKKME
                     AESGRIIKI"
BASE COUNT          802 a          644 c          831 g          755 t
ORIGIN      
        1 ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc acaggtgctg
       61 acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttat gatggccctg
      121 tgggggcctt tgtccggcac cagttacggc attgccgggc tgctgatgct ggcagtcagc
      181 caccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg
      241 gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc tggtcttgtg
      301 atgacggtgc tgaccgttgg tctggtgtcc tgtgcaggaa aatcattacc ccgtttctgg
      361 cccggggatt ttttcccggt attctacgcc tgtcatctgg ctgtgctggg cgttctggcg
      421 ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt cgtcagaggc agaacacagc
      481 atgacagagc agaagcgacc ggtactgaca ctgaagcgga aaacggaagg ggagacgcct
      541 gtccgcaggc ggaaaaccat catcaatgtc accacgccac caaagtggaa ggtgaaaaag
      601 cagaagctgg ccgagaaagc agcccgggaa gcggagctgg cggcaaaaaa agcgcaggcc
      661 agacaggcgc tgtccattta tctgacgctg ccctcgctgg atgaggcggt gaacactctg
      721 aaaccctggt ggccgggatt gtttgacggt aacacgccca ggcttcttgc ctgtggtatc
      781 cgggaggttt tactggatga ggtgtttcag cggaatatcc cgctctctca taaaaaactc
      841 agccgggcgc tgaaagctat cacccgttcg gaaagttatc tgtgtgcaat gaaagccggc
      901 gcctgccgct atgatacgga aggctatgtg acagaacata tcacacagga agaagaacag
      961 tatgcgcagg cgcgcctgga aaaggtgcgc agacagaacc ggatcaagga tgagctgcgg
     1021 gcgatcctgg cggaataaaa gagccctctg tatgacagag ggcaacagcg agaaaaaatc
     1081 gggaaaacag aaatccagag ttttgatgga gaaaacaccc aaccgttcag ggatgtttca
     1141 gaatgtttct gacataatcc gggagccggg tcaagagggg ggataaaaaa tggctgtatc
     1201 cagtggatta tctgagcaca gcggtattta tcgctatcag cgcgtggggc gtcatgccgt
     1261 ccgtcaggag attcgtggtt ttttcaggga agccgctgag gcctggcaga atgcggcccg
     1321 gctggcaccc tgtgaagcat ggcgggattt tgccctgaag agggcatctg catgccggtg
     1381 taagtgccga taaaaataac ccccgtcgcg gatctccggg ggatcaggct tatatggcag
     1441 atcgttatta ttatagtggt gtaagaagga gttgccctgt ctttcaaacg gacatcacag
     1501 agttaacata tttcacatcc cgggtaagcc agcagggata aaaaatgtga tgaatttcaa
     1561 gaagcagaag gtgacagtag catgcataac agtacggtta tgtccggtga atctgacaga
     1621 cagccgggct gtattcgcgg caggataaag aaaaaccggg agatgactct cccggtccgt
     1681 cttatcgcta atcccctgag cagggcatgt atgacttatc tatgaccgtt atattcagcg
     1741 ccacatccgg tagcctgcga tataacacag ttatcctatc agtaagagca ggtatgatga
     1801 atacacggag attaaaaata taatctccgt cacatgttac ccccggaaac cggtaacgcc
     1861 gtcacgggaa atacgtgaca gaaaaagcag cctccgttac tccaggtatc agaaggctga
     1921 ataatgcatg agactttgtt tcttatgtgt ctggtcaggc tggctgacca gcgtttttag
     1981 taacacatag ccatatgtgt attaagccac ctcatcttat tttgtcgaat tgatcacaat
     2041 tcaacggttt atttttggcg tgatacgtga gccgttattc atcccggcat ggaattcatg
     2101 caatcacgaa aagtaatcag tgacggagag taaaatgaag aaatatatac cagtggtatt
     2161 atttgttttt tcatgtcagg tattttctgc tgatattcat ggacgggttg tgcgtattct
     2221 ggatggtgat accattgacg tcatgttatc tcagcatccg gtgcgtgtcc gtcttgttaa
     2281 tattgatgca cctgaaaaga aacaggacta tggccggtgg tctgaaaaaa taatgaaatc
     2341 tctggttgcg ggaaaaacgg ttactgtcac atattttcag cgagatcgtt atggtcgtat
     2401 tctcgggcag gtttatgcac cagacggaat gaatgttaat cattttatgg tccgtgcagg
     2461 cgctgcatgg gtttatgagc agtacaacac cgatcctgtt cttcccgtcc tgcagggaga
     2521 agcacgacag cagaaacgtg gtctgtgggc agacagcgct cctgttccgc cctgggtatg
     2581 gcgctcacgg aaataatgat atttctgtgg ctattgagtt ctcattgaaa tgcgatattt
     2641 aaaagcatga agcatattac attattaact tatttctctg ttaatgaaag gtgaacaaat
     2701 gaactatcag acagtatcag aattaatcac atcgtctaat cataatgttc ttatagtgtg
     2761 ggactcagct tctgaagttg atggtttttt aaataaactg aatatcactg attataagta
     2821 ttatgatttc agccagattt acagttgttc tgacagaaca ttaaatgatt atgcggttat
     2881 tttttatcag ggatggcctg atttgaataa tgaatctgcc gtcatgcagt ttgccagagt
     2941 ggcacgaaaa tcaggaaaac aactgatagt cgcagtaaga gaacaggata tgaaaaaaat
     3001 ggaagcagag tctggaagga tcataaaaat cc
//
LOCUS       DAZFRE010000223         3005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFRE010000223 DAZFRE010000000
VERSION     DAZFRE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.228"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            796..975
                     /gene="hha"
                     /locus_tag="ACHKUI_005075"
                     /pseudo
     CDS             796..975
                     /gene="hha"
                     /locus_tag="ACHKUI_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
     gene            complement(1308..1505)
                     /locus_tag="ACHKUI_005076"
     CDS             complement(1308..1505)
                     /locus_tag="ACHKUI_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601074.1"
                     /translation="MVICKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            1704..2273
                     /locus_tag="ACHKUI_005077"
     CDS             1704..2273
                     /locus_tag="ACHKUI_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6601075.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHSRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            2440..2823
                     /locus_tag="ACHKUI_005078"
     CDS             2440..2823
                     /locus_tag="ACHKUI_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6601076.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            2820..>3005
                     /locus_tag="ACHKUI_005079"
     CDS             2820..>3005
                     /locus_tag="ACHKUI_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6601077.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQK"
BASE COUNT          748 a          744 c          823 g          690 t
ORIGIN      
        1 agataaacgg ggggattgcg gtggctgagc cgtaccggta tgtcgggtga ttgttgcgaa
       61 acgatgcttc cgcacgcgca cgtgataaag gagggaggac tttcgtgaga caaggggaaa
      121 actggcgttc tgataattgc agagttgttt ttgaaggcgg ttgtggtgca tcagcccgtg
      181 acctgtgggc aggcgacagg tatcactggt gggataagac tgtctgattg gtattataaa
      241 tatataagag agctcaaaca catgagttca tatatgtgaa ataaatctgt gagtgtgatc
      301 agcagaccag tctacccagt agcaacagat tgcgttgtac tcacagggtt atccctgata
      361 actggcgtat aacctgcgta cataaaacgt acctgcaatg cacctgaaca gagtgcatta
      421 agcatccgcc tgagcggtaa acgtatccga tctggtgagt gacagtctga gagcattctc
      481 agtaatcgcc cccttgtatc ctggaaattg gttaagataa cagcgattag atttggcaag
      541 gtgtatgcag ccagcagctt aacatctcag ctaactggct ggttaccttc aggttgtcgc
      601 aatggcatcg gacatccggt gtcggataac taaacgtagc gttaaggcga tattgctgag
      661 agaccggtta agtggttgca cgggagtaat gagtttgggg agggtaccag aatgtcagtc
      721 tggtgcggta tgctggcagt atcacgttga ttacagcata aagtaacgag agacgaatgg
      781 aagttaaaac cagagcagtt ccgtcgttgt tcatcccggg agacgctgga aaaagtgatt
      841 tcccacacac gttataaact tacccctgcg gagctggaag cctttaactc tgcggtcgat
      901 caccggctgg cagaactgac aatgaacaaa ctttacgatc gcgtgccggc ttccgtctgg
      961 aaatatgtca cctgaatctt gaatccgcgc tacgatgact ccggtgttat ctgtgcaccg
     1021 gaagatcggg aacattaagc agtataacgg caacaaagaa ttcgaaatca aaagggaatg
     1081 gtgtacagag gggcaggcgt tcaggcatta ccggcctgta tcttctgaag gggactcgta
     1141 tttgtcgggg ctgagtattc cgctcgattc gatcacggat tggcggaata cctgactgtg
     1201 attgattcag gtaccatttt gcagggagcc ggtgcgttac tgtctggcac aacaaacgca
     1261 gcgggtcgct ctgccccgcc gattcctgat aatccctcct ctgtaaccta ctgcaacagt
     1321 acaaatacac tgacgcctga aacggtaacc agaatcagca ccagcagtaa cgtcagtaaa
     1381 cagagcagac gatcggtctt ccggtaataa ccgaaggggt cgacagcgcc acaatacggg
     1441 caacatactg tattatgagt aattgcgtta ccacagtccc tgcacttctt tttgcatatc
     1501 acgatcgctc cctctccacg ccttatccgt aacctgtttt ttacattaaa aaaacaacgc
     1561 cgggaattca atcgtcagtc cggcgatgac cgttcgggtt atcacagagt gcctgagaca
     1621 gtgtcctgcc ggaggtcacc tcaggggctc tgcgcatttt ttacgacgtg gttattccgt
     1681 atgaaccata cggagattta accatgacct acaaatacaa ccccttctgg cagcaacgta
     1741 ttcgtgagac ggtgcggcac gcactgaatg ttcattcccg cctgacggca ttgcgggttg
     1801 acctgcgttt cccggatgta ccggcagcaa cggacgcagc tgtgatatcc cgcttcatca
     1861 atgccctgaa agcccgaatc gacgcttacc agaaacgtaa gcatcgggaa ggtaaacgcg
     1921 tgcatcccac aaccctgcat tacgtctggg cccgggagtt tggggagtgc aaaggtaaaa
     1981 aacactatca cctgatgctg ctggtcaacc gggatacctg gtgtcgtgcc ggcgattacc
     2041 gcgctccggg atcactggcc gggatgatta aacaggcgtg gtgcagtgcc ctgggagtgg
     2101 atgccggacg ttatgacacg ctggcacatt ttccggtcag gccggcggtg tggctggaac
     2161 gcgatgatga caccggcttt cagcaggtgc tggagcgtgc tgactatctg gcgaaggaga
     2221 gtaccaaagc ttacggcacc ggtgagcgca actttggctg cagtcgtggc tgagcccgac
     2281 agacgactta tctgacctta gtgtgatgac gtgttcacag cgaccacact gattttccct
     2341 tctgagactg aactgaaaac cgccattcac acctgaccac gctgcccgta ccgggacggc
     2401 tgttgcctgt gtgtcgtctg gcgcagagga acatatacta tgtacgcaaa atcttttctc
     2461 gcacttgatg gcaacggacg tctgaccggt gcccgtaccg cacaaactgc tccttatgcc
     2521 cactacacct gccacctttg cggcagtgca ctcagatacc acccgcaata cgacaccgaa
     2581 cggccctggt ttgaacacac tgacgacggg ctgacggagc acgcgcagca atgtccttat
     2641 gtcaggccgg aacgcagaga aatacggttg attaaacgtc tgcagcaatt cgtcccggat
     2701 gccttacccg tggtacgtaa agccagctgg tactgcaggc aatgtcacca cgattattat
     2761 ggggagcagt actgcacaca ctgccagacc gggcgtttca gtgaggaggg ggtagcagaa
     2821 tgaacggtat aaaaacgggg ctggggatca cgccgggtga gcacattatc agtgccgact
     2881 ctgcgctcag cagaaatatc cggcattgtt tctgcctttc ctgtcatggc cggttgattc
     2941 tgcagacaga tgctcagggg gcgtggtttg agcatgattt acatgccctg agtgagcaac
     3001 agaag
//
LOCUS       DAZFRE010000224         2981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFRE010000224 DAZFRE010000000
VERSION     DAZFRE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.229"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>2981
                     /gene="traI"
                     /locus_tag="ACHKUI_005080"
     CDS             <1..>2981
                     /gene="traI"
                     /locus_tag="ACHKUI_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO6601078.1"
                     /translation="KIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQAI
                     REAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRA
                     EIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERA
                     RAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPR
                     TAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQ
                     MNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQ
                     VLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYDRLAGDFAA
                     SVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLR
                     DMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLF
                     RPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADS
                     PFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDET
                     RTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKN
                     LAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASY
                     EAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAG
                     VGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQ
                     QRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQP
                     FRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGA
                     WAPEHSVTEFSHSQEAKLAEA"
BASE COUNT          645 a          792 c         1014 g          530 t
ORIGIN      
        1 aaaatatatc agagtgagtt acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg
       61 gtcggtaagc acggtatgtg ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt
      121 tcacaggcta tcagggaggc cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg
      181 gcactggata cgcgaaaatc caaacagcac gtcgatccgg aaatcagaat ggctgaatgg
      241 atgcagacgc tgaaggaaac cgggttcgac atccgggcat atcgtgacgc ggcggatcag
      301 cgtgcggaga tccgcacgca ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag
      361 caggcggtga cacaggcgat tgccggatta agtgaacgaa aagtgcagtt cacgtatacg
      421 gacgtgctgg ccaggacggt cggcatactg ccgccggaaa atggtgtgat tgaacgggca
      481 cgcgccggta tcgatgaggc catcagccgt gagcagctta tccccctcga ccgtgagaag
      541 gggctgttca catcaggaat tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt
      601 gacatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggct
      661 ggttacagcg atgcggtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
      721 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
      781 gagcaggggc gggaggtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
      841 caggatgaac ggctgtccgg tgagctgata accggacgtc gtcagctgca ggagggcatg
      901 gtcttcacgc cgggcagtac tgttatcgtc gaccagggcg aaaaactctc cctgaaagag
      961 acgttaaccc tgctggacgg tgccgcacgt cataacgtac aggtcctgat aaccgacagc
     1021 gggcagcgaa ccggtacagg cagtgcgctg atggccatga aggatgccgg ggtgaacacg
     1081 tatcgctggc agggcggaga acagcgaccg gcaaccatca tcagtgaacc ggaccggaat
     1141 gtccgctatg accggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
     1201 gtggcacagg tcagcggggt acgggaacag gccatactga cacaggccat tcgcagtgag
     1261 ctgaaaacac agggcgtgct cggacacccg gaggtgacca tgaccgccct gtcaccggtc
     1321 tggctggaca gccggagccg ttatctgcgg gatatgtacc gaccggggat ggtgatggag
     1381 cagtggaacc cggagacacg cagtcatgac cgctatgtta tcgaccgggt gacggcgcag
     1441 agtcacagcc tgaccctgcg ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc
     1501 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggcgagcga
     1561 ctgagggtga cagggaaaat tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg
     1621 gcatccgtca gtgaagatgc gatgacggtt gttgtgccgg ggcgggctga accggccacc
     1681 ctgcctgtgg ctgattcacc gttcacggca ctgaagctgg agaacggctg ggtggaaacg
     1741 cccgggcatt ccgtcagtga cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg
     1801 gacaacgcca ccctgaacgg tctggcccgc agcggtcgtg atgtccggct gtattcctca
     1861 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
     1921 gagcagataa aggcgcgggc cggtgagaca tcgctggaaa ccgctatcag tctgcagaaa
     1981 accgggctgc acacgccggc acagcaggcc attcatctgg cccttcctgt gctggaaagt
     2041 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa
     2101 ggaaccggtt ttactgaact gggaggggaa atcaatgcgc agataaaacg cggtgactta
     2161 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
     2221 gaggcagaaa aaagtattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
     2281 atggagagag tacccggcga actcatggag acgttaacgt cgggacagcg tgccgccacc
     2341 cgtatgatac tggaaacgtc cgaccgtttc acggtggtac agggttatgc cggtgtgggt
     2401 aagaccacac agttccgggc ggtgatgtca gccgtgaaca tgctgccggc gagtgagcgt
     2461 ccccgagttg tggggctggg gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc
     2521 gtggatgcac agacgctggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
     2581 gaaacgccgg atttcagcaa cacgctgttc ctgctcgatg agagctcaat ggtgggcaat
     2641 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgtgc cgtggccagt
     2701 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct ccagcagacg
     2761 cgcagtgctg ccgatgtggt catcatgaag gagattgtgc gtcagacgcc ggaactgcgg
     2821 gaggcggtat acagcctgat taaccgggat gtggaaaggg cactgtccgg gcttgagagt
     2881 gtgaaaccgt ctcaggttcc ccgtcaggag ggcgcatggg caccggagca ctccgtgacg
     2941 gagttcagtc acagccagga agcgaaactg gcagaagcgc a
//
LOCUS       DAZFRE010000225         2975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFRE010000225 DAZFRE010000000
VERSION     DAZFRE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.230"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(183..308)
                     /locus_tag="ACHKUI_005081"
     CDS             complement(183..308)
                     /locus_tag="ACHKUI_005081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601079.1"
                     /translation="MKRVAIPAPALRKSALSPMLATLPLIFLLTPLVCQLFVIIS"
     gene            complement(305..538)
                     /locus_tag="ACHKUI_005082"
     CDS             complement(305..538)
                     /locus_tag="ACHKUI_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6601080.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(607..1182)
                     /locus_tag="ACHKUI_005083"
     CDS             complement(607..1182)
                     /locus_tag="ACHKUI_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601081.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVVGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRDVVLDSRSPDCENILKELIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
     gene            complement(1529..2260)
                     /locus_tag="ACHKUI_005084"
     CDS             complement(1529..2260)
                     /locus_tag="ACHKUI_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6601082.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSETRKASDKKSGGSAVLWVKRGDVEAMQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGVGRLLDGR"
BASE COUNT          852 a          754 c          696 g          673 t
ORIGIN      
        1 aacaaaacgc cggatgtctc ccggctctga aattaaggct tcggttattt gcacgaaatg
       61 ttccttttca aaccctgttg aattttgtta agccggtgca gacacacgga aaacgcacat
      121 caacaccgga cggtacgata caaacaccag tgactggcgg gacgaaggga atgacgaatc
      181 agtcaggaaa tgatgacaaa cagttggcag accagaggag tcagcagaaa gatgagcggg
      241 agtgtcgcca gcatcgggga cagggctgat ttccgcaatg ccggcgctgg tattgcaaca
      301 cgtttcatgc tgctgctcct cgtgattcct caattcgctg ctgcatccac tgctccactt
      361 cacttttgta ccagcggctg ctgcgcccca gtttgatggg tttagggaaa tgaccttcac
      421 tgatcagctt gtaaaaccat ttatcggtca taccggtgaa actggtgatg tagttcatgt
      481 cgatcagggg atcgtcggca cgcagtaaca cgctgtcgtg gcttgttgtg gtcaacatgg
      541 tcagaatgtc ctttgtgaga tttatgaagt ttgtgcgaat gcagggaaag atgctgaacc
      601 gagctttcag aacggtcctg cagatccggg atagccggga ttaccggcga ggcaacgggc
      661 ttccggatcg cacacaccac gacgatcgcg ggcgaaatca ctgaacgccc atatcaactc
      721 tttgaggatg ttttcacagt ccggtgaacg actgtccagc accacatccc gggtgatctg
      781 cttctgatac tgctttgctt cacccggtga cgcgaagccc agtacgttac tccaggcatc
      841 cagtatcaac cggtccaatt cagggccatc gaaatactcc tgtctgacgc acagcaccac
      901 gtggtattcc ggtttttcag gatgacgggt caggagccag cagagatcac gtccgggggt
      961 tgcattcatc agcgcactga cggtttcgat aaaatccata ccaaaccagg tcgttctgct
     1021 gttgaaggta ttgtcatatt cacacggcag acgaagcagg atccgccgta tccgtgagcc
     1081 ggactgctca cgcagcgcac gctcaaggac gccgacgact ttgttctgat aagatggcca
     1141 gaagggggcc aggctatagt cgaaataagg gatagcattc ataagatatg ctccgttatg
     1201 cgcagcccac aatgcgtcct gcccgtgaat aagctgacgt gttatggttt gcggatgggg
     1261 ttgatggaat aagtgaagaa ccgaagcaga acgggaagat aagggaaaga acactggatg
     1321 caggttcagg gggaatgatc tccgttgact tcggttcaca tcattaatca tcccggtaag
     1381 tttttaccga cggagtgttt ccaggagcgg aagcagataa aaataccgtt tcatgacttc
     1441 agggctatta gcagaaaacg atctaagggc gttggctgac gcccttaccg tgacatacac
     1501 tctggtaccg gatgccgttt tacagggctc acctgccatc cagtagacgc cctaccccac
     1561 gactgcaacc ataattacgt tgcccggaac cgtcatgctg cttcgtctca tatttcacga
     1621 gataactggc tctgttccgg gctttctgca tcgcttccac atcaccacgc ttgacccaga
     1681 gcacagcgga tccaccagac tttttgtctg aagccttcct ggtttcagaa catccacccg
     1741 acaaaggcgt atcatcggaa gaagggcgag catcagatga tgtcggttta cggaaaggcg
     1801 tccgcctgga aaaatgaacc agtccattac cctgccaggg ctcgagatgc agagcactgc
     1861 accatgccag ttggatcagc gtcgccagcg aagaaggaac ggtgaaatcc cctggcgagc
     1921 accaggtatc tttgttgagc agtaatatca cgtgataatg tttcctgcct ttttccttgc
     1981 caaactcccg acaccagaaa taacgcagag tggttgcacg cacacgcttg ttggcacgtc
     2041 gtttatgtcg ctggtaagcc tgaatttttg ctttcaggga tgcaaaaaaa cgagaaatca
     2101 ccgctgaatc taaatcggta tccatgatgg tggcaggcat atactcaggg aaacgtaaat
     2161 ccacccggat taacgagacg gcacggtgtt gcgccagggc actgttgaga atactttcaa
     2221 aacgggataa ccagtaagca ttgtgtattg gttgattcat gatgagagat ctccgttgat
     2281 ttggattgac ttcacatcat taatcactgc ggtaaaactt tacggcatcc cgccattgag
     2341 caacgccaat ggctcctgat taaaacagaa tgaatccccc acgaagaaaa aataaaccta
     2401 cacgcagagt aaaacacaca acaacggcat aaatgttttt taaaaactac aaagcctgaa
     2461 gggaaacgga tcaacagaac gcgcaaacaa aacgtcagaa acgcaatcag aacgctttct
     2521 ggcgctgtaa atcaacagca acaccacacc tacagcttaa ccacacaact caatcacaga
     2581 gccgaacagc gcgattaaaa cctgtttaca gacacccccg ccccaagaac aaaaaccacc
     2641 aacatcagaa tcgattttct tatactgaaa acgttacttt taactacgct ccataaaaac
     2701 ggttaaccat cgataacgta aacctcatct gaatcaaaac accagaacca atcatcacgc
     2761 ttttcctgaa aagagtcttg cgctgagaaa aaacaccagg gccagttaat catctcatca
     2821 atctcatcgt tacaaaatca tccataaaac catttaacaa aatcatcgta agcacacagt
     2881 aaatcacgcc aggttatctc agccggattt gcgtttttac ataaagacac ttgtctcagg
     2941 ttaacgacta aaaacacaaa aactaagaaa aggtt
//
LOCUS       DAZFRE010000226         2966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFRE010000226 DAZFRE010000000
VERSION     DAZFRE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.231"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            509..1999
                     /locus_tag="ACHKUI_005085"
     CDS             509..1999
                     /locus_tag="ACHKUI_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6601083.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(2120..2692)
                     /locus_tag="ACHKUI_005086"
     CDS             complement(2120..2692)
                     /locus_tag="ACHKUI_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601084.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2778..>2966)
                     /locus_tag="ACHKUI_005087"
     CDS             complement(2778..>2966)
                     /locus_tag="ACHKUI_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6601085.1"
                     /translation="LMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKLGRSSRW
                     LKSEVEAWLQARITQSRP"
BASE COUNT          778 a          643 c          735 g          810 t
ORIGIN      
        1 gctggcgatt atacagaagt atttgaagaa aaaatgttgc attttatctg tgtcagcagg
       61 gaggaagtat tttattgatg tatcagtatt aaatggtttt tgttatttat aaaaatgtca
      121 tcctgtccat agaatattgt actcagcatt cagtgcagga actgtcattt tctttatttt
      181 attcttcttc aggagggcag ttttctgaaa gcgttactcc ggggaaactg tttatgttta
      241 ttcccggcag gaaacccgta cctgcaatac cctggatgat gctttttgct ggtacgttgt
      301 tgtgtgaaat ttaatcaacg tgtgtgttcc ggagagcgcc cgtagacgca cagattcatt
      361 ctgacattct ttagcgacaa aataccgaat ggttattatt taatctgaaa atcgacagaa
      421 atttttatcc tgtattgatt ctgctgctat ttttgctatt agtagcaata gttgcggtac
      481 tttatgttct gtgtgttgtg aggtcattat gccatacttt cagtatcctg atgagtttcc
      541 cctgagctcg ctgcctccac tcattcgtga tgccgttatt gaagctcagc aaatcacgca
      601 ggctcccctg gggcttgtgg cagcatctgc cctgggcgct gtttcacttg tctgccagaa
      661 tctgattgat gtctgcagac tgaatacact acgggggccg gtttctcttt tcttactgac
      721 gcttgctgag tccggagaac gaaaaaccgc agtggacaaa ttattaatgg agcccctgta
      781 ccagcaggag atgctgttat attccagaca caaaaatgaa ctgacgacat ggaaaaataa
      841 ggaagagctt ttaaaggcgc agaaaaaagc attgttgtca aaactgaata aagaactccg
      901 caaaggcgcg gatgagtcag aaacgctcag acaactggaa gcgcttcaga aaaacagagg
      961 ggaaaaacct gtaagatata aattcatttt taatgatgcc acgactgcgg caattaagaa
     1021 tcagctttgt ggtaaatggc gttcagtggg tattatgtca gatgaagccg gtataatatt
     1081 tgatgggtat accctgtctg agctgccttt tattaacaaa atgtgggatg gctccgtatt
     1141 gtcggtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa tgacattatc
     1201 cctcatggta caacccggtc tttttgaccg atatatggag cgaaaaggtt ctgttgccag
     1261 ggacagtggt tttcttgccc gttgtctaat cagtaaacct gccaccacgc aggggaaacg
     1321 ctttattaac ggagctgtga ccccgggagg atcattaacg gcatttcatg aacgattaat
     1381 ggagctggcc agaggcagta ttgaaaaatc cagtaaggat gaacgttatt gtctgcattt
     1441 ttctcctgaa gcgcaaaaaa tatttattga tcactataat gttctggagc aggatttgtc
     1501 tccttccgga cccttaagcc agtttcgggg acatgtttca aaaaaaacag aaaacattgc
     1561 gagaattgct gcgttattgc agtatttcag tcagggtgaa gggaaaatct ctgccgatat
     1621 catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa aattatttgc
     1681 gttacctgat gaatctgaac tgcagctgca ggacgcccgg gagctgctcg actggctcat
     1741 tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc tccagtgtgg
     1801 cccgggccgg ttccggaaca ggaaaaagct gaacgtcctg ctgaacattc ttgcatctca
     1861 actcaggctc tctgtcgtgc aggaaggaaa aaccacctat gtcctgcttt ccgaaataaa
     1921 caatatcacg ctgcctgaac tgaatgcgtg ctacagccag aaactgataa gatggcatga
     1981 gcaatccttt ggcttataat gttccctgtt gctgagacgg attattcagt ggtggtatcg
     2041 cagtatgccc gtcacccccg cttctgcatg agggtgacgg aggaaaagta agtgtgcgga
     2101 ggcccgggag cgttatgaat caacaggcgt ataacaggta ctcgtcaatc atcagttcca
     2161 gctcgcgctt ttcccgtttg cgcgccacag cccagttttc aagctctgca tgtgatttat
     2221 gctttaactc ttcagtccag ggctgcatgg agtgggcaca catcagatca taccagcaca
     2281 gccatatcag cgtgtggtag agatcacgat cgtcgaaacg ccggagcagg ctgttcagcc
     2341 tgtttgccag ccggttttca cccagacgat gtagctcact gagtaaaacg ccgcccagat
     2401 taaaatgtgt aagcagcatg atttcatggg tctgaatgct ggtcattaac ccttcctgaa
     2461 tggcaggaat gaacggtggt gttcccgggt tgacgacctg atagagtccc tgtggtgcca
     2521 ctgagatgat ggtgcctttt tctggcagaa aaaagtgggc cagggtcagg cggtgataac
     2581 catgaagatg cggagccccc attccatgag cgacgataag cctcgtcacg tttgtcatcc
     2641 atttctgatg ctggatgagc agcagctcac accggttttc aagtttttgc ataatattct
     2701 ctcctttagc gcgcagcaaa atgcctccgg gggccggatc atgcctttgc tgcggttgaa
     2761 cggataacgg tcaggaatta cggacgggac tgtgtaatac gtgcctgcaa ccaggcttcc
     2821 acttcacttt tcagccatcg ggagctacgg cccagtttga tgggggcagg aaaggcacca
     2881 tccttgatga gcttgtaaaa ccatttatcg gtcaggccgg tcagctgagt gataaacgac
     2941 atgtcgacca tctggtcatc catcag
//
LOCUS       DAZFRE010000227         2921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFRE010000227 DAZFRE010000000
VERSION     DAZFRE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.233"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            137..2134
                     /locus_tag="ACHKUI_005088"
     CDS             137..2134
                     /locus_tag="ACHKUI_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO6601086.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2197..2535
                     /locus_tag="ACHKUI_005089"
                     /pseudo
     CDS             2197..2535
                     /locus_tag="ACHKUI_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
     gene            complement(2545..2921)
                     /locus_tag="ACHKUI_005090"
                     /pseudo
     CDS             complement(2545..2921)
                     /locus_tag="ACHKUI_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          647 a          714 c          735 g          825 t
ORIGIN      
        1 gtgtccgcta ttgccagtac acctcaacat tccaccatgc attccgatta acgccgcata
       61 gccagttgaa ctttgctact ttgtgagagg tagtaccttc tatccagtgc gaatttaatt
      121 aatggaataa atgattatga gtgaaaatga tacaatccca aagaagtcta caagtcagat
      181 taacaaagcg gtattcttta catctgcttt gctaattttc cttcttgtcg cctttgccgc
      241 cgtattcccg gatgtcgccg acaaaaattt taaactactt cagcaacaaa tcttcacgaa
      301 tgccagctgg ttctacatcc ttgctgtggc cctgatttta ctgagtgtca cgttccttgg
      361 actctcacgc tacggtgata tcaagctggg cccggaccat gcgcagcctg atttcagcta
      421 ccactcctgg tttgcgatgc ttttttcggc agggatgggg atcggcctga tgttctttgg
      481 cgttgccgaa cctgtaatgc attatctttc gccacccgtt ggcactccag aaaccgttgc
      541 ggcagccaag gaagcaatgc gtctgacctt tttccactgg ggactgcacg catgggcaat
      601 ttatgccatt gtggcgctga ttctggcgtt cttcagttac cgtcacggtc tgcctttaac
      661 tctgcgctcc gcactctatc ccattattgg cgatcgcata tacggacctg taggacatgc
      721 ggttgatatt ttcgctgtta taggcacggt ctttggcgtt gcgacatcac tgggttacgg
      781 tgttttgcag gtgaatgccg gtttgaacca tcttttcggg gtgcccatca atgaaacggt
      841 gcaggtaatt ctgatcgtgg tcatcacggg gttagcgacg atttcagtgg tgtccggtct
      901 ggataaggga atacgtatcc tgtctgaact caatctgggt ctggctttgt tgctcctggc
      961 gctggtcctg tgtctgggac caaccgtgct tctgctgaag tcatttgtgg aaaatacggg
     1021 cggttatctt tcggaactgg tgagtaaaac gttcaacctt tacgcgtatg agcccaagtc
     1081 gagcaactgg ctggggggct ggacattact gtactgggga tggtggcttt catggtcgcc
     1141 gtttgtgggg atgttcatcg cacgggtctc ccgcgggcga accattcgcg agtttgtcac
     1201 cggcgtgctg tttgttcccg cgggttttac gctaatgtgg atgacggtgt ttggtaacag
     1261 cgcgatctat ctcattatga accagggggc cacagacctc gccaatactg ttcagcagga
     1321 tgtgtcgctg gccctgttta atttcctgga gcatttcccg ttctcttctg tgctgtcatt
     1381 cattgcaatg gcgatggtca tcgtcttctt tgtaacgtct gctgattcgg gggcaatggt
     1441 tgtggatact ctggcatcag gtggagtggc aaacacaccc gtctggcagc gaatattctg
     1501 ggcctcgctc atgggcattg ttgcaattgc gcttctcctt gccggagggc taagtgcgct
     1561 gcaaacggtg acaatagcga gtgcattgcc cttctcagtg atcttactaa tatccatata
     1621 cggactttta aaagctttgc gccgggattt gaccaagcgt gaaagcctga gcatggcgac
     1681 aattgctcct acggctgcac gtaacccaat tccttggcag agaaggttac gcaatatcgc
     1741 gtatctgccg aagcgatctc ttgtgaaacg ttttatggac gacgttatcc agcccgccat
     1801 gacgctggtt caggaggaac tgaacaagca ggggacgata agccacatta gtgatgcagt
     1861 cgacgatcgt attcgtcttg aagtcgattt gggcaacgag ctgaatttta tatatgaagt
     1921 gaggcttcgc gggtatatct caccgacctt cgcgctcgcc gcaatggata atgatgagca
     1981 gcagactgaa caacatcgat attatcgcgc tgaggtttat ctcaaagaag gcggtcaaaa
     2041 ttatgatgtg atgggctgga accaggaaca gctgattaat gacatactgg accagtacga
     2101 aaaacacctg cacttcctgc acctggttcg ttaatagcaa catgccgtcc tgggggcggc
     2161 aattattatc tcggccgcaa tatgagggaa tgcagaatga tttcacgctg gaaatggatg
     2221 ctgaagcaga caattaaaaa actatggttc agggcaacgt tattcgcaat tgtcgcgata
     2281 ataacggccc ttttatcaat tctttttaaa tcaatgatac ctgagtcggt ttccgtgaag
     2341 gttggtgcgg aagcagtcga taacattctg aacatactgg catcgagtat gctggcagtg
     2401 accacatttt cgctgagtat catggtcaca gcctacggtt cagccactac taatgtgact
     2461 cccagagcta cgcgtttagt tgttgaagac gtcaccacac aaaatgtact ggccaccttc
     2521 atcggttctt ttctcttgaa gtggtcaaca aaaactggcc accgcgttag agtttttcca
     2581 gtatcgattt tccgattcgt ttggggtaac ccaccgttat attcgtgcgg tcttagtgcg
     2641 ctgtaatatc caacgatata gtccgttatg gcgtgagctg cctcgctgaa gcttacgtaa
     2701 cccaccaccg gcatccattc gttcttcaga ctcctgaaga agcgttccat tgggctgtta
     2761 tccctgcagt ttccgcgccg gctcatactc tgtctgatct ggtatcgcca caataactgc
     2821 cggaactgcc tgctcgtata atgactgccc tgatcgctgt ggaacatcac cccgccgggc
     2881 ttaccacggg tttcccatgc catttccagc gctttcatgg t
//
LOCUS       DAZFRE010000228         2847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFRE010000228 DAZFRE010000000
VERSION     DAZFRE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.234"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..2546
                     /gene="sat"
                     /locus_tag="ACHKUI_005091"
     CDS             <1..2546
                     /gene="sat"
                     /locus_tag="ACHKUI_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HFO6601087.1"
                     /translation="GNGKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAA
                     TDDGTTITNSDTTKEAVLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGS
                     VNTKNDVEVSNASLTMQGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKF
                     NSDHKSNNQQTSFDQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGD
                     KNAYIDNLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNG
                     ATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADG
                     FRLSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFD
                     TAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTI
                     DDSAFVLRANLAQADQLVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVD
                     VFKATTRSIGFSDVTPVIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFL
                     AEVNNLNKRMGDLRDINGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLD
                     LFTGVTMTYTDSHAGSDAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTAT
                     FAGLGTRDYSSHSWYAGAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLT
                     MKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIK
                     GEKDGRMLMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT          886 a          611 c          633 g          717 t
ORIGIN      
        1 gcggaaatgg taaggtaata ctaaataaag ataattccct tggcaatgat caatatgcgg
       61 ggatattttt tactaaacgt ggtggcacgc tagatttaaa tggacacaat cagactttta
      121 ctagaattgc cgccactgac gatggaacaa caataactaa ctcagataca acgaaagaag
      181 ccgttctggc aatcaataac gaagactcct acatatatca tgggaacata aatggcaata
      241 taaaactaac gcacaatatt aattctcagg ataagaaaac taatgcaaaa ttaattctgg
      301 atggtagtgt caacacaaaa aatgatgttg aagtcagtaa tgccagtctt accatgcaag
      361 gccatgcaac agagcatgca atattcagaa gcacagcgaa tcattgctcc ctggtatttc
      421 tttgtggaac ggactgggtc accgttttga aagaaacaga gagttcatat aataaaaagt
      481 tcaattctga tcacaaaagt aataatcagc agacctcatt tgatcagcca gactggaaaa
      541 ccggggtgtt taaatttgat acattacacc tgaacaatgc tgacttttca atatcacgca
      601 atgccaatgt tgaaggaaat atatcagcaa ataaatcagc tatcacaatc ggcgataaaa
      661 atgcttacat tgataatctt gcagggaaaa atattactaa taatggtttt gacttcaaac
      721 aaactatcag tactaatcta tccataggag aaactaaatt tacaggtggc atcactgcac
      781 ataacagcca aatagccata ggtgatcaag ctgtagttac acttaatggt gcaacctttc
      841 tgaataatac tcctataagt atagataaag gagcaaaagt tatagcacaa aattccatgt
      901 tcacaacaaa aggtattgat atctccggtg agctgactat gatgggaatc cctgaacaga
      961 atagtaaaac tgtaacgccg ggtctccact acgctgctga tggattcagg ctgagtggtg
     1021 gaaatgcaaa tttcattgcc agaaatatgg catctgtcac cggaaatatt tatgctgatg
     1081 atgcagcaac cattactctg ggacagcctg aaactgaaac accgactata tcgtctgctt
     1141 atcaggcatg ggcagagact cttttgtatg gctttgatac cgcctatcga ggcgcaataa
     1201 cagcccccaa agctacagtt agcatgaata atgcgatctg gcatctaaat agccagtcat
     1261 caattaatcg tctagaaaca aaagacagta tggtgcgttt tactggtgat aatgggaagt
     1321 ttacaaccct tacagtggac aaccttacta tagatgacag tgcatttgtg ctgcgtgcaa
     1381 atctggccca agcagatcag cttgttgtca ataaatcgtt gtctggtaaa aacaaccttc
     1441 tgttagtcga cttcattgag aaaaatggaa acagcaacgg actgaatatc gatctggtca
     1501 gcgcaccaaa aggaactgca gtagatgtct ttaaagctac gactcggagt attggcttca
     1561 gtgatgtaac accggttatc gagcaaaaga acgatacaga caaagcaaca tggactctga
     1621 tcggctataa atctgtggcc aacgccgatg cggctaaaaa ggcaacatta ctgatgtcag
     1681 gcggctataa agccttcctt gctgaggtca acaaccttaa caaacgtatg ggtgatctgc
     1741 gtgacattaa cggtgagtcc ggtgcatggg cccgaatcat gagcggaacc gggtctgccg
     1801 gcggtggatt cagtgacaac tacacccacg ttcaggtcgg tgcggataac aaacatgaac
     1861 tcgatggcct tgacctcttc accggggtga ccatgaccta taccgacagc catgcaggca
     1921 gtgatgcctt cagtggtgaa acgaagtctg tgggtgccgg tctctatgcc tctgccatgt
     1981 ttgagtccgg agcatatatc gacctcatcg gtaagtacgt tcaccatgac aacgagtata
     2041 ccgcaacttt cgccggcctt ggcaccagag actacagctc ccactcctgg tatgccggtg
     2101 cggaagtcgg ttaccgttac catgtaactg actctgcatg gattgagccg caggcggaac
     2161 ttgtttacgg tgctgtatcc gggaaacagt tctcctggaa ggaccaggga atgaacctca
     2221 ccatgaagga taaggacttt aatccgctga ttgggcgtac cggtgttgat gtgggtaaat
     2281 ccttctcggg taaggactgg aaagtcacag cccgcgccgg ccttggctac cagtttgacc
     2341 tgtttgccaa cggtgaaacc gtactgcgtg atgcgtccgg tgagaaacgt atcaaaggtg
     2401 aaaaagacgg tcgtatgctc atgaatgttg gtctcaacgc tgaaattcgc gataatcttc
     2461 gcttcggtct tgagtttgag aaatcggcat ttggtaaata caacgtggat aacgcgatca
     2521 acgccaactt ccgttactct ttctgataac agcccgggcc gcgtttgcgg cccttcttct
     2581 accggagaga atatgtatta ccctgtgaca gactatatcg ctcttgctct cattattagc
     2641 tttctttttc tgacattatt tatctgcctg ttatgcctta aacatgagcg aataaaaaaa
     2701 gaaactatca ggcaaaaaaa tgcacatatt ctggatcatg gctggaatgc aactgaggta
     2761 gcctgagttt aacggacact ccttcctgaa atagaatggc atcagaagga gctaataatg
     2821 agcagaaaaa cccaacgtta ctctaaa
//
LOCUS       DAZFRE010000229         2760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFRE010000229 DAZFRE010000000
VERSION     DAZFRE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.235"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1172)
                     /locus_tag="ACHKUI_005092"
     CDS             complement(<1..1172)
                     /locus_tag="ACHKUI_005092"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO6601088.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNSFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMMLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTRLVSVIKNINQG"
     gene            complement(1172..2752)
                     /locus_tag="ACHKUI_005093"
     CDS             complement(1172..2752)
                     /locus_tag="ACHKUI_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO6601089.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
BASE COUNT          853 a          633 c          511 g          763 t
ORIGIN      
        1 tctccctgat taatattttt aattacactc acaagacgcg taagccccat atataatttt
       61 tctcgaccgc aggccaggtt cagacgaagg tagccctccc cctccgttcc atagacatgc
      121 cccgccataa tgcccacttt gccttcatga attaatcgac tgtagaattc atcaaaagga
      181 taaccaatag cagaacaatc tatccaggaa aaatagcaac catcagctgg ccttacgatc
      241 aattctggaa tattttcatt cagatactgc tgggtgaatt gaatgttaga ggctatatgt
      301 gcctttaatg actttaacca tccctcacat tcgttgtagg caataatagt tgcaattata
      361 ttcagtactg atggggagtt tacaaaatcg cgatacctcg atatcgttct gaacttatga
      421 ttaaaattat cgtcagttga aatcatatac gatcctgtta gtgcaggaac attaaatgtt
      481 ttcgatatcg agttaaatac tactactttt tcttttttta ttttaagcgc ggatgtaaag
      541 ctacgattaa aaacaaaatc actatggatt tcatctgaac ataaccagac attatgcctt
      601 gtacagcttg cagcgatttt atgcagttct tcttccgacc agactgttcc tgttgggtta
      661 tgaggattac ataataacat catctttgcg cttttaatct taagatcaaa atcttgccag
      721 ttgatagcgt agcccccctc attttttata atcaaagagg ctgtcagaat atttcgctcg
      781 ctatgtttaa ttacatcaaa aaatgaatta tagacaggtg taaaaacgag aatattatca
      841 ccatgcgatg tcaataactc tattgctttt gcaatagagt acatcacact ggggctatag
      901 gttatccatt caggtttaat atctgtttga tattgttgat aataccagtt tactatcgca
      961 ttcagatatt caccatgttg ccaacgactg taccctaaaa tcgggtgttc caggcgtttt
     1021 tttaatgctc tggtgatagc gtctggtatc ggaagatcca tatctgaaat agagaatggt
     1081 agtacatctt ttacaccaaa tctgtcctcg caaaagtccc attgggtaca atatgttcct
     1141 ttgcgggaaa caggagtatt aaaaacatcc atcagtatat ccccatatag ttatccatat
     1201 cccgacgcag ggcgatgact ttagtgccga taatgacttg tagggtattt tgatcaagtt
     1261 taacaaccgc aatcccgcca gctcttttta ttgcagcctc gtcaatcaat cccatatctg
     1321 cgattgttaa acgtaaacga gttacgcaat tatccagtga cttaatattc tctttaccac
     1381 caagtgcctt aaccatctgc tccgccgctt gcagctctga tttatcagga gttgggatat
     1441 ccgctatagc cgtatcttcg tgagcgttca gcgtgacagg actggactct tcacggccta
     1501 cggtcatcag gttaaattta agaatggtcc aacggaacac aaagtaataa atgaagaaat
     1561 aggccacttc gacacatacc gcgatcggcc aacctgttct tgtgccacgc aatacgccaa
     1621 agctaataaa ttgaatcaga tcaccagtaa atcccatctt cacgcccata tatggcagaa
     1681 ccatgttagc gaatccattc ataaaggcat ggaagacaaa cagcagtgga ctgacaaata
     1741 agaacagaaa ctcaatcggt tcgctgacgc cgccaataat caccgtaagc accccggaaa
     1801 taagcaatgc tttgatagcc tttctgtttt gagcatgagc agtgcgatac atcgccagcg
     1861 cggcaccagc cagaccatac tgaatcatca gtttgccctg ctccattttc cccgccaaat
     1921 ccagcggaat gaccgcattc tctttgacgt atgccataaa catgttcagg gtgccgtaat
     1981 attcttcacc gccaatcaca gccgaaccgc cgattggcgt aaagcggaac actgacgtca
     2041 ccagatggtt taagccaaaa ggaatcgtca cacgttcggc aactgcatag aagaaataac
     2101 caacaggacc ggaagttgat atccagtggc caatgagcat gaagagattg aaaaatggcg
     2161 gccagataaa aggaatgatc agaccaaatc cactcatcac aatcagtgta atgataggca
     2221 ccagacgtgg gccgctataa aaacctaatg attctggaat gcgtaaatta acgatctttt
     2281 tatacatgct ggcgactaat aacccagcaa caattccccc caacacgctg gtattgtagg
     2341 actggatccc cagaatgatg gtttgcccat gtgtcgacat ttggtcagca acgaccaata
     2401 agtcgtgctg tttaagataa aagttcgttc ccagatgcat cgccataaaa ccaattaagc
     2461 cagaaaaagc accataggct ttatcctctt tatcttttaa taatcctaag ggaatcgcta
     2521 tcgcaaacaa tacaggtaaa ttaacaaagg caaacaaacc aagactaaca atgaaatcaa
     2581 gtatggtttt aattattgga atagccagaa atggaattaa ctttgccata tcatcactgg
     2641 ctaaaccact tcccagccct agcatcatgc cacatacact tagcagagca atgggataca
     2701 taaatgcctt ccccaggctc tgaaaaaaac tccaggcttt cttttgtttc atgtgggtta
//
LOCUS       DAZFRE010000230         2741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFRE010000230 DAZFRE010000000
VERSION     DAZFRE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.236"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHKUI_005094"
     CDS             complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHKUI_005094"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO6601090.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIANGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPF"
BASE COUNT          649 a          724 c          643 g          725 t
ORIGIN      
        1 aaacggcgcg aagatttcgc taaatgcgta cttcggagcc tgttctttag tcagaccgtg
       61 gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc agataccaac
      121 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
      181 cacctcaccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tggcgtcaat
      241 ttctgcatca ctcttattgc catagaggcc tttcagctgc gcatgcaggt tggcaatcgc
      301 agacgttgca cacttaccgt ggatgttctg gccttcggtg tttgcagact gacaaatagc
      361 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
      421 atcgctcagg acattcatcc cttccggcgt caggcctgtc ggcagaattg gcaaggtata
      481 ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct tcacagcaag
      541 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
      601 gactctgagg ttcaggttat caacgatgtc ataataatgc tgcattagat cgtcaacgtt
      661 cttacaggaa gctgccagcg gggcattatt agcaccaccg atagacagca tcacatcacc
      721 gccagcctca cgcagagctt tgattttgct gtactgagcg taattctgca taccgtaagc
      781 ggtaccccat gtcggtagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
      841 gtggttgacg ttatgattct tagccagcgc agccaaatcc gggatcgtat tcagcgtaaa
      901 gtcgacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc
      961 tttggttccg gtccagtcaa caaaaacacg ccacgggttg ctgtcagaag gagaaacttt
     1021 ctgcacttta ctctgagaaa tgtagttctc accgttaaag cgaatcagcg tatcactggc
     1081 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc tatagctctg
     1141 agtcttacct aacggcttcc acgggcgact attgctaccg gtggcgtttt ggttcgcaac
     1201 cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
     1261 aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg gttcgtattt
     1321 cttccacggg ttatcgccgc cggggacaaa gccccatgcg tcttgatcga cagaggtttt
     1381 ataatgtgca tcgttataat ctacgatgct gttcgcgctg tacgctttgc tggcctggaa
     1441 accattcgca ataatgacgc cgccattatc aatttcacag gtagtcgggt taccgtactg
     1501 actcatttcc gcagcggtag ctgtacgcac gaggcgccat gggttattgg agttttctac
     1561 actggtgcca cgcgggcaat cttcagaacc aacccaccag gcattctgat aaacattacc
     1621 attgaagata acatggtaaa tttcgctgcc ttcctggcca ctccacgcct gcagtgagta
     1681 atcgttactg ctatccgctg gtgttgatgg ctcgttgtta tccggagttg gagtaactgg
     1741 agtttccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
     1801 cttaacggaa caggagcctg ggttaccata ctgagtgatt tctgttgccg ttgccgcgcg
     1861 aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1921 gcccacccac caggcattct tataaatgcc gccgttgtag ataacgtacc atgtagaaga
     1981 accttcctga cctttccagg ctacgacagc atagttgtcg tttgacttat cagctggggc
     2041 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gtgcaggctg
     2101 tggtgctgaa gtacaagact gtggattact ggtttctgag atttccgttg ccgtagcggc
     2161 acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg ggcagttaga
     2221 agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat accaggtttg
     2281 accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag tgccctgtgc
     2341 tggtgtagcc gaaccgccat tagcaggcgt attagatgct ggcgtatttg aatttgaaga
     2401 agatgagctg ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
     2461 tgttgcggta cgctttaaac gccacgggtt agttgcatca tttgctttcg cttttcccgg
     2521 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
     2581 atatttatta ccaccttgtt ggttattcca tgcttccatt gccaatgctg gcagagcgga
     2641 acacaccagc cccataccaa tcatagattt agtaaatata tttaatttca ttataatccc
     2701 ttgtaacgta aaaactgcaa aacaaaagtc ctacgcagga a
//
LOCUS       DAZFRE010000231         2739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFRE010000231 DAZFRE010000000
VERSION     DAZFRE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.237"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(186..476)
                     /locus_tag="ACHKUI_005095"
     CDS             complement(186..476)
                     /locus_tag="ACHKUI_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601091.1"
                     /translation="MGIVFTNHNIDLLSVEFDEITKNCNYTFSVDGETAIFTARISII
                     RNIKGIKYSEELDKFIMSIMPLQPKVSKILGGVTWDCICGKEVGFPVRLIGK"
     gene            complement(451..1890)
                     /locus_tag="ACHKUI_005096"
     CDS             complement(451..1890)
                     /locus_tag="ACHKUI_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFO6601092.1"
                     /translation="MGNYHIGDGECRFRVVCSSPDVCEVGGYKVPFDSYQTLDSERQY
                     SSTVWARGCRALNVGSVIAGTQSNAGKGVISGTSQGTGDCVILTGSPTVTIEGKPVAY
                     HGSVVGINNHNCLGKLYTKIKSPMISVIDRTFNYERTAEVIHDLLLLKDLLSVGNIFD
                     GDISPEVKNNLFQIKDPDQSWGEFFSIKNIRESLRNGIEGDKSQIREWFGENTLTQMG
                     NGAITTLHGVADLALVTFDALLDTATATVACPIGEDGLCEQANINLNEKEQALFNISN
                     SLINGQAWDALKKMIMDTNNGDQIALEHFASFLWGFMIPAKIPEENISGKVFVEPVVL
                     EGGAGGNWTVFDEVLDSNVIKQLTLTGCGAACGEMLLRDRYIFVTQNVIGTELTSMTS
                     LANKLNKFDVGWEGNAVSESSLYALSNTGSWGAMMWDSGSKVGHWVLVKGVDDAGNVI
                     IYDPYQGSRYLMTEQEFKEVWNGHSVYKP"
     gene            complement(1883..>2739)
                     /locus_tag="ACHKUI_005097"
     CDS             complement(1883..>2739)
                     /locus_tag="ACHKUI_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO6601093.1"
                     /translation="SLKSETDLVLCKRNTDIHVTGSAHAPSGDKSQWKACVRVNSFSK
                     ELSLSGVRYLQYERNRWQMSLPDKIINVPLRYELAYGGIWQPDGMEKLVFSANPVGCG
                     YYPDISQLNTSCQYKLPQITSSALSENATIFNGESDFFQGVGPVSRWWKSRLQYAGTY
                     NEVWREKRYPYLPDDFDERFYNSAHPDMIYTGFLSGDENISLEGFFKFEQVVKTKLPG
                     IRPVLILKTKNNTSHMFLPVADTMVIDLSRQEIYLTWRLTIPDFFGMKEGVLSCIIPE
                     CIGKKYYG"
BASE COUNT          862 a          640 c          382 g          855 t
ORIGIN      
        1 aacacctcaa ttattgaaca taaaatgcga attatttagt acaaaaaagc agcattactt
       61 gacctttccc tgcataacaa tatttataaa ataatacgtt ttatgatctc accttgggct
      121 ccaaacaaga taatttcagt tttaaagact ttcacgaaaa aaatatagaa atacctcacc
      181 acccttcact taccaattaa tctcacagga aatccaactt ccttaccaca aatacaatcc
      241 caagtaacac ctcccaaaat ttttgatact ttaggctgca atggcattat tgacattata
      301 aatttatcaa gttcttcgct atatttaatt ccctttatat ttcttattat tgatattcta
      361 gcagtaaaga tagccgtctc tccatctaca ctaaaagtgt aattacaatt ttttgtaatc
      421 tcatcaaatt caaccgataa caaatcaata ttatggtttg taaacactat gcccattcca
      481 tacctcctta aattcctgtt cagtcatcag atatcgactc ccttgatatg gatcatatat
      541 tatcacattc ccagcatcat caacaccctt aacaagtacc caatgcccta ctttacttcc
      601 agaatcccac atcattgctc cccatgaacc agtattactg agggcatata aactagattc
      661 gctcactgca ttcccttccc agccgacatc aaatttatta agcttgtttg ccagcgacgt
      721 catagatgtt aattctgttc caattacatt ttgcgtgaca aaaatatacc tatccctaag
      781 caacatttcc ccacaggccg ctccacagcc tgttaaagtc aattgcttta ttacatttga
      841 atctaaaacc tcatcaaata ctgtccaatt gccaccagca ccaccttcta aaactactgg
      901 ctctacaaat actttcccag agatattttc ttctggtatt tttgcgggta tcataaagcc
      961 ccataaaaag ctggcaaaat gctccagagc aatctgatca ccattatttg tatccattat
     1021 cattttttta agtgcatccc atgcttgccc atttatcaat gaattactaa tattaaataa
     1081 agcctgttct ttttcattta aatttatatt tgcctgttca cataacccat cttcgcctat
     1141 cgggcaagca accgttgccg ttgccgtatc taacagagca tcgaatgtaa ctaaggctaa
     1201 atccgccaca ccatgtaaag tggtgattgc tccattcccc atttgagtaa gtgtgttctc
     1261 tccaaaccat tctctgatct gagatttatc tccttcaata ccattcctga gagattctct
     1321 gatattcttt attgaaaaaa actcccccca tgattggtca ggatctttta tttgaaataa
     1381 attattttta acctcaggtg aaatatctcc gtcaaaaata ttaccaacag agaggagatc
     1441 cttcagaagc agtaaatcgt gtattacttc agcagtcctt tcataattaa aagttctatc
     1501 aatgacagaa atcattggtg attttatttt ggtatacaat ttaccaaggc agttgtggtt
     1561 attaatccca acaactgaac catgataagc aacaggtttc ccttctattg ttacagtcgg
     1621 acttcccgtt aaaataacac agtcacccgt tccttgagat gtaccggata tgacaccttt
     1681 accggcatta ctttgagttc cagcaatcac tgaaccaaca tttaaagctc tgcatccacg
     1741 cgcccatacc gttgaactgt actgtctttc actatctaaa gtctggtaac tgtcaaaagg
     1801 aaccttgtaa cctcctactt cacatacatc tggtgaagaa cacacgactc taaatctaca
     1861 ttcaccatcc ccgatatgat agttacccat aatatttttt ccctatacat tcaggaataa
     1921 tacaactcag cactccttct ttcattccaa aaaaatcagg tattgttaat ctccaggtaa
     1981 ggtatatttc ctgtcgactt aaatcgataa ccattgtatc cgcaacaggt aagaacatat
     2041 gtgatgtatt attcttggtt tttaatataa gtacaggtcg tatacctggc aattttgtct
     2101 taacaacctg ttcaaattta aaaaaacctt caagagagat gttttcatcg ccagagagaa
     2161 accccgtgta tatcatatcc ggatgagcac tattgtagaa ccgctcatca aaatcatccg
     2221 gcaaataggg atagcgcttt tcacgccata cttcattgta ggttcctgca tactggaggc
     2281 gacttttcca ccatcgagat acagggccaa caccttgaaa aaagtcagat tccccattaa
     2341 aaatggtagc gttttctgac aaagcagagc tggttatttg tgggagttta tattgacaac
     2401 ttgtattaag ttgagaaata tcaggataat atccacaacc tacagggttt gctgaaaaaa
     2461 ccaatttctc cataccatct ggttgccaaa taccaccgta agcaagctca tagcgtagag
     2521 gaacattaat aattttatct ggtaagctca tctgccaacg atttcgctca tattgtaagt
     2581 agcggacacc agataaactc agttctttag aaaaactatt aaccctcaca caagccttcc
     2641 actgagactt atcccctgaa ggggcatgag cactacctgt cacgtgaata tctgtatttc
     2701 ttttacaaag aactaagtct gtttcagatt ttagactac
//
LOCUS       DAZFRE010000232         2725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFRE010000232 DAZFRE010000000
VERSION     DAZFRE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.238"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..809
                     /locus_tag="ACHKUI_005098"
     CDS             <1..809
                     /locus_tag="ACHKUI_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6601094.1"
                     /translation="LAIVLPAIGLVGCDRVEPGNVGIKVNKLGDDKGVGEVVGVGRYW
                     TGWNTEVYIFPTFKQMKTYDDPFSFQMSDGTTIGYHIGVAYKVDPSKVTTVFQTYRKG
                     VDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTPIGIQVIS
                     LSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQADAIRTK
                     AQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            851..1222
                     /locus_tag="ACHKUI_005099"
     CDS             851..1222
                     /locus_tag="ACHKUI_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO6601095.1"
                     /translation="MNTHNTQPQIMNYDPNLTSCGRMAKQTVRLTFGQWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGETDIMATINIGELTCQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            1254..1496
                     /locus_tag="ACHKUI_005100"
     CDS             1254..1496
                     /locus_tag="ACHKUI_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6601096.1"
                     /translation="MFALINQGQLYTDSAGYPVIIIRCINNTVLYRRIDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            1500..1646
                     /locus_tag="ACHKUI_005101"
     CDS             1500..1646
                     /locus_tag="ACHKUI_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601097.1"
                     /translation="MNKAFELWVRQRYGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            1655..1891
                     /locus_tag="ACHKUI_005102"
     CDS             1655..1891
                     /locus_tag="ACHKUI_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6601098.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRARKECWMVGREYI
                     HVSPDGNPKPSSECMYNRKAIDAWVASMKNKQPR"
     gene            1947..>2725
                     /locus_tag="ACHKUI_005103"
     CDS             1947..>2725
                     /locus_tag="ACHKUI_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6601099.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGSFDYAAQFPDSPNLQAFGVSKKEITVKELEEKWLDLKRMEISAN
                     AFNRYESVARTMVPKIGGSKLVSSVTKEELLYIRKDLLTGYQNSTKNKAAAKGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQ
                     TKNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKL"
BASE COUNT          823 a          605 c          687 g          610 t
ORIGIN      
        1 ctttagccat tgttctgcct gccattggcc ttgtcggttg tgatcgtgtt gagcctggta
       61 atgtgggcat caaagtcaac aaacttggcg acgacaaagg cgtcggtgaa gtggtcggtg
      121 ttggtcgcta ctggacaggc tggaacactg aagtttatat cttcccgacc tttaagcaga
      181 tgaagaccta cgatgatcca ttcagtttcc agatgagcga tggaacaacc attggttacc
      241 acatcggagt agcctacaag gttgatccat ccaaagttac cacagtcttt cagacctacc
      301 gcaaaggcgt ggacgacatt accgacaccg acctgcgcca gaagatagcc gacgcactca
      361 accgactggc cagcaaaatg accaccgaca aatttatcga cggcggcaag tctgagctgc
      421 tggatgcagc tcttaaagac attcaggaag aaatgacgcc catcggtatt caggtaataa
      481 gcctctcata tgtgggtaag ccggagtacc cgcctactgt tatcgacagc attaatgcca
      541 aagtcacggc gaaccagaaa actctgcaac gcgagcagga agtaaaacag cgcgaagcgg
      601 aagccaacat gttgcgcgcg gaagctgccg gacaggctga tgcgattcgc acaaaagccc
      661 aggctgaagc cgatgctatt cgtttacgcg gtgaagctct gcgtcagaac cccggtgtta
      721 tggagctgga agccatcaat aaatggaacg gcacgctgcc gcaatacatg accagcggtg
      781 ccaatacacc atttatccag gttaaataac ttaatatgcc cggcaggccg tcgggctaag
      841 ggaaaagcag ataaacactc ataatactca accgcaaata atgaactatg acccaaatct
      901 gacgtcatgc ggacgcatgg cgaaacaaac cgttcgatta actttcgggc aatgggaata
      961 ccgcgaaaca ttcgaagtca ctgtcggtgg caacctgacc ggactcgatg ttatcagttg
     1021 cgctattgaa agcctgtacg caacgctgcc ttatgaagaa gtcgaggatg agcgtacagg
     1081 ggaaacagat atcatggcca ccattaatat tggcgaactg acatgccagg atgaagacct
     1141 gtccggagag ctctggcttg ccgggatgct tatctcagca gaaattatca gtattgaacc
     1201 cgctacaaac atacggctct gaagttctca ctattcagag agcaggagaa aaaatgttcg
     1261 ctttgattaa tcagggacaa ctgtataccg acagtgccgg ttacccggta ataattattc
     1321 gctgcataaa caacactgtg ttgtacagaa gaattgatgg gcgaacacag tcagtaaaaa
     1381 taaacgattt caatgaactg tttgaacgga ttgatcacca ggaataccga caaattctgg
     1441 cagaaacaga gcaggaggct catctgaaaa aattacgggc catgaaaagg aagtaaagaa
     1501 tgaataaagc gtttgagcta tgggtgcgcc agcgttacgg caatcgctat gacctgacgc
     1561 gagatgttga cggcttctac tgtcgtgaaa ttgtgaaacg aatgtttgaa gtgtggtgcc
     1621 actgccgtgg gctgagtgtt gtgtgaggta atacatgggc aatgtgattc aactggctcc
     1681 caatgaatgg gtttgtgaaa gcgttcttat cgcaattacc gggctcaaac caggcacaat
     1741 tcttcgggcc cggaaagaat gctggatggt tggaagagag tatattcacg tatcaccaga
     1801 cggtaatcca aagccttcca gtgaatgtat gtataacaga aaagcaatag atgcctgggt
     1861 cgcctcaatg aaaaacaaac aacccaggtg atttaatatc atgaaatatg taagctcgta
     1921 tcgctcttgg gcgtctggag gtatcgatgg ataaagtcaa atatccaaca ggcgtcgaaa
     1981 accacggcgg cacattacgc atctggttta attttaaagg taaacgtgtc agggaaaatc
     2041 ttggtgtccc tgacactgcc aagaacagga agatcgccgg ggaactgcgg acatcagtat
     2101 gttttgccat ccgcacagga agctttgatt atgctgcaca gttccctgac tcccccaacc
     2161 ttcaggcttt tggggtaagt aaaaaagaaa ttacggtgaa ggaacttgaa gaaaagtggc
     2221 tggatctgaa acgaatggaa atctctgcaa atgcattcaa tcgctatgaa tccgttgcaa
     2281 gaacgatggt tccgaaaatt ggaggcagta aactggtgtc atcggtaacc aaagaggaat
     2341 tgctgtatat caggaaagat ttgctgaccg ggtatcagaa ttcaacgaaa aacaaagcag
     2401 cagcaaaagg acggagcgtc gttactgtaa attattacat gacgacaatc gctggaatgt
     2461 ttcagtttgc tgcagatcac ggttacttag aagcaaatcc cttccaggga attaagcctc
     2521 ttaaaagagc cagggcagag ccagatccgc taactcgtga cgaatttatt cgcctgatag
     2581 atgcttgccg acatcagcag acgaaaaacc tgtggtcatt ggctgtgtac acaggaatgc
     2641 gtcacggtga actggtctcc ctggcctggg aagatatcga tctgaaggca ggaacaatta
     2701 ccatcaggcg caattatacg aaact
//
LOCUS       DAZFRE010000233         2711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFRE010000233 DAZFRE010000000
VERSION     DAZFRE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.239"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            447..590
                     /locus_tag="ACHKUI_005104"
     CDS             447..590
                     /locus_tag="ACHKUI_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6601100.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            603..812
                     /gene="cspE"
                     /locus_tag="ACHKUI_005105"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             603..812
                     /gene="cspE"
                     /locus_tag="ACHKUI_005105"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6601101.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            978..1787
                     /gene="rlmA"
                     /locus_tag="ACHKUI_005106"
     CDS             978..1787
                     /gene="rlmA"
                     /locus_tag="ACHKUI_005106"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6601102.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPLRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1784..2350)
                     /gene="mntP"
                     /locus_tag="ACHKUI_005107"
     CDS             complement(1784..2350)
                     /gene="mntP"
                     /locus_tag="ACHKUI_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6601103.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          735 a          612 c          633 g          731 t
ORIGIN      
        1 agcgaaacgt ttctctcttg atttttttgc tcattcatca atttttctta ttttaaattt
       61 acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa ctgactgttt
      121 tatgagaaaa agtgatataa ctttttattc attgcatagt agaaaatgtg atattgcacg
      181 cactatgtaa taacttctcc cactggcctg gaacaactga acttattgaa ctatgttaga
      241 aaatacgtca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg atatatcggc
      301 gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct
      361 gatcgagaca tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat aacagatttc
      421 gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta
      481 aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc tcatgcagta
      541 atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc
      601 aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt tttggcttca
      661 ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc cagggtaatg
      721 gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac ggtcagaaag
      781 gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga agtgtgaata
      841 cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactttg ttttatcttc
      901 gctccagtcg ttcggatatt tgcccgccgc atgattcgcg ttacacttgc ggcctttagt
      961 atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct ctttcgcgtg
     1021 aaaaaaacag ctatatctgt cccctgcgac atcagtttga tatggcgaaa gaagggtatg
     1081 tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc gcggaaatga
     1141 tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt gatgcaattg
     1201 tcggccaact gcgggaacgg cttgatgcaa aggccgcggc ggtgctggat attggctgtg
     1261 gtgaagggta ttacacacac gcatttgctg atgcgttgcc cgaaatcacc acgtttggtc
     1321 tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag gtcacttttt
     1381 gtgttgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc ataatacgta
     1441 tttacgcgcc gtgtaaagca gaagaactag cgcgagtagt gaagcccggc ggttgggtca
     1501 ttactgccac gccgggaccg agacacttaa tggagctgaa ggggctgatt tacaatgaag
     1561 tacatcttca tgcacctcat gcagaacaac tgaaaggttt tacattacag cagagtgatg
     1621 agttgtgtta tctgatgcgt cttcgcggtg atgaagccgt cgcattattg cagatgacgc
     1681 cgtttgcctg gcgtgcgaag cctgaagtct ggcaagcact ggcagcaaaa gaagtgttcg
     1741 actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg tggaagtgcg
     1801 tccagaggat ctggacgcca ataccgatca gtaccagccc gccgagaatt tccgcttttt
     1861 tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac ataatcaagg
     1921 ttgcacaacc aatggccaat gcggtcgcga taatgttaac ctgcaggaaa gcaagaccaa
     1981 cacccacagc catggcatcc aggctggtgg caatcgcagt ggttaccagt aaccagaaac
     2041 cgtgtcgacg gcgcggttct tcatcttcat catctgcgcc acgaaaaccc tcaataatca
     2101 ttcgcccgcc gagaaatatc agcagcacaa acgcaatcca gtggttccat tcaaggacaa
     2161 accgactggc taacatgccc attccccagc cgatcagcgg cgtcagggtt tcgacggcac
     2221 caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg gtggcacctt
     2281 taccgattga tgcagcaaag gcatccatcg acataccaaa cgcaagaaga acagtcgcag
     2341 tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg cccccagcaa
     2401 acatgcatac atgaacaaca agccgcgatg gttttcgcgt tcatgcaggc atagcgattg
     2461 atgtgcatat ggtctcgcct gactgaaagt gttcagtccg tacgtgccac gttttgcaac
     2521 gagtatgttg acacgtacat ttccggaatc tggaaatcag ctactcccca aatgagggcg
     2581 caaccttaac atattttgag aatataagac aactgttgga atatattatt ttattgtttt
     2641 attgataacg attttcattt atatttatat atcaatgaaa cgttaaaata aaaaacaaat
     2701 aagggaagag g
//
LOCUS       DAZFRE010000234         2701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFRE010000234 DAZFRE010000000
VERSION     DAZFRE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.240"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            313..1185
                     /locus_tag="ACHKUI_005108"
     CDS             313..1185
                     /locus_tag="ACHKUI_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6601104.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1557..>2701
                     /locus_tag="ACHKUI_005109"
     CDS             1557..>2701
                     /locus_tag="ACHKUI_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6601105.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMG"
BASE COUNT          652 a          712 c          755 g          582 t
ORIGIN      
        1 ttaatatctc ccactgacat ctcccccaca atacactaaa aaatactcac agcagtaaac
       61 acagatacac ctgataatca tacagccgat aaatattttc ccctttacgg agaaatcagc
      121 ctgctgttta acaataacgg cgtattttca tggaaggaga tcacccagta acggataagt
      181 cctgatctcc ttcatgttaa tacgcaacaa tcccatcgat tcaaaacaat ttcagacaga
      241 tattatctcc gtgaatcccc cgccaccttt ccggtgcgcg gggttttgtc ttttttcacc
      301 gggaatacat gtatgaatcc gtctgatgcc attgaggcaa ttgaaaaacc gctctcctcc
      361 ctgccttact cactttcccg tcacatcctg gaacatctgc gcaaactcac cagtcacgaa
      421 cccgtgattg gcattatggg taaaagcggg gccggtaaat cctcactctg taatgcactg
      481 tttcaggggg aggtcacccc ggtcagtgat gttcacgccg gcacccggga agtacggcgc
      541 ttccgtctga gtggccacgg tcacagcatg gttatcactg acctgcccgg ggtgggcgag
      601 agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg acattctgcc cgaactggac
      661 ctggtgctgt ggctgattaa agccgatgac cgcgccctgt ctgtggatga gtatttctgg
      721 cgacacatcc tgcaccgggg acatcagcag gtgctgtttg tggtgacgca ggccgacaaa
      781 acggagccct gccatgaatg ggatatggcc ggcattcagc cttctcccgc acaggcacag
      841 aacattcggg agaaaacgga ggcggtattc cgtctgttcc ggcccgtaca tccggttgtt
      901 gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg tcagtgcgct aatgacagca
      961 cttcccgacc atgccgccag tcccctgatg acccgcctgc aggacgagct gcgcacggaa
     1021 tctgtccgcg ctcaggcccg tgaacagttt accggtgcgg tggaccggat atttgacacg
     1081 gcggagagcg tctgtgttgc ctctgttgcc cgtacggttc tgcgtgccgt ccgtgacacg
     1141 gtggtctctg ttgcccgcgc tgtatggaac tggattttct tctgaacagt ccgccaggga
     1201 aatgcctgat aacattacgt taaccaactg aaaacatgac acaaacccct catactcctc
     1261 ctgtttctct tgcgtgaccg ccttgtcgtt aatagaataa aacgatcgat aaaacaggta
     1321 tcgatcgtta ctatcgatcg tttatagtgg ttaatggtct gcgaaaaggc agctgaatct
     1381 cttcatcatg cagaacggaa ttgcacacaa cggactgata cttctctggc ggatgagaga
     1441 ggggaagcat ttatgtccgg gaaaaaccac tttatctgag catgctgttt tgtacctgcc
     1501 ggtatccaca tttgtgggta ccggcttttt tattcaccct ctgtaaggaa aagctgatga
     1561 aacgacatct gaacaccagc tacaggctgg tatggaatca cattacgggc accctggtgg
     1621 tggcctccga actggcccgc tcacggggaa aacgcgctgg tgtggcggtt gcgctgtctc
     1681 ttgctgctgt cacatcagtc ccggcactgg ctgctgacaa ggttgtacag gcgggagaaa
     1741 ccgtgaacga cggaacactg acaaatcatg acaaccagat tgtcctcggt acggccaacg
     1801 gaatgaccat cagtaccggt ctggagtatg ggccggataa cgaggccaat accggcggac
     1861 aatggataca aaatggcggt atcgccaaca acactactgt caccggtggt ggtcttcaga
     1921 gagtgaatgc cggaggaagc gtttcagaca cggttatcag tgccggaggc ggacagagcc
     1981 ttcaggggca ggcagtgaac accactctga acggcggtga gcagtgggta catgaaggcg
     2041 ggattgcaac gggtaccgtc attaatgaga agggctggca ggccatcaaa tccggcgcag
     2101 tggcaaccga cacggttgtg aataccggcg cggaaggggg accggatgcg gaaaatggtg
     2161 ataccgggca gaccgtctac ggagatgccg tacgcaccac catcaataaa aatggtcgtc
     2221 agattgtggc tgctgaagga acggcaaata ccactgtggt ttatgccggc ggcgaccaga
     2281 ctgtacatgg tcacgcactg gataccacgc tgaatggggg gtaccagtat gtgcacaacg
     2341 gcggtacggc gtctggcact gttgtaaaca gtgacggctg gcagattgtc aaaaacgggg
     2401 gtgtggccgg gaataccacc gttaatcaga agggcagact gcaggtggac gccggtggta
     2461 cagccacgaa tgtcaccctg aagcagggcg gcgcactggt taccagtacg gctgcaaccg
     2521 ttaccggcat aaaccgcctg ggagcattct ctgttgtgga gggtaaagct gataatgtcg
     2581 tactggaaaa tggcggacgc ctggatgtgc tgaccggaca cacagccact aatacccgcg
     2641 tggatgatgg cggaacgctg gatgtccgca acggtggcac cgccaccacc gtatccatgg
     2701 g
//
LOCUS       DAZFRE010000235         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFRE010000235 DAZFRE010000000
VERSION     DAZFRE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.241"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(160..1340)
                     /locus_tag="ACHKUI_005110"
                     /pseudo
     CDS             complement(160..1340)
                     /locus_tag="ACHKUI_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            1927..2156
                     /locus_tag="ACHKUI_005111"
                     /pseudo
     CDS             1927..2156
                     /locus_tag="ACHKUI_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          650 a          653 c          666 g          683 t
ORIGIN      
        1 taacctcata aaaaatcctg tactcacagg gtgatgcggg ataactgacg taaggagtgc
       61 tgcagggatt tgatggtccc ttgtgctgat atgaatacgg atattttata cagaatactt
      121 ttcagtacaa gcgactgtat tacagtctgc catatatgat tacctgtgat caggaagaat
      181 aagtggctga ggtttcaaac tgtctgccgg tatattcctc tctcccttaa aaaccataac
      241 aggttagtta tcttcgtctg tcagcggatt gaatggcggt atattttcag tacaaatacc
      301 ggtcatccac tgaaaaatac ctgcgaaatg acgggcactg atttttctac tgacgggctg
      361 atgagacgtt atgtcactgg cggtaataat cagtggcaca ctgtagcctc cctgcacatg
      421 accataatga tgaacaggat tggcactgtc gctgaccgac agcccaaggc cagaaaagta
      481 aagcatggcg aaatgacgtg agtaccggcg aaggatacca tcaagctggc caagaaagtt
      541 atccagttta ctgatgctgg cgaggtaaca ggcaattttt cggggatact gctccaggta
      601 attattcggc taggagttaa ggctgtcaca cggatttgga tgagaaccca tcatgtgcag
      661 gaaaattatc ttcggagagg atgtatccgc cagcgcacgt tctgtttcct gtaacaacag
      721 tttgtcatct gttttacggg aagcgaagct gcctttcttg aggaaagtgg catgctccgc
      781 atcagaagca ataacagaga tgcgtgtgtc atgctctccc agttttcctt gattggatat
      841 ccaccatgtg ctgtatcctg cttttgctgc cagcgccacc acattgttgc cggaatcagg
      901 gttctgctca tagtcataaa tcagtgtccg gctcagggaa gacactgtac tggaggctgt
      961 cgaggtataa tcgtcaataa ataaaccggg tgccgtattc agccacggtg tggttggtac
     1021 gggatagtca tacactgaca tataatccct gcgcacactc tcaccagtga caataacaat
     1081 cgtatcatat aacggtgctc ccggctggat tttccagttg tcagccccgt gctactcagt
     1141 tgttcataac gctgcatttc acgtaatgtg tcagttgtcc ccacaactgt tcctttaacc
     1201 atccgcaatg gccaacggtt tacagagcat aatacgaata gccgtagagc cagccagtta
     1261 cggtggtcgc gatggtgtgt ccgccagaaa atcaccacga ataccagaat cgcggcactg
     1321 accagaaaat gataaacagg aaacacgggg cggttcactt tgctttctcc tgtagtgttt
     1381 tctgtaggtt ctgaaccggt tctagcgggg ttgtgaggct gatggcaatc gccgggctct
     1441 tcctgcatgt tacactggta ctgcaagagg gggccttcgc tcctctgaca ttactcagta
     1501 ttttcactac tacaccccat ctgccatcca taacaaccga ttcaccccct cgcgaggcgt
     1561 tgttgcccgt cacccggaca gacaccgtta tggatttctc ttgttgcacg atcgatcaat
     1621 tgttatgcat gctgacacca caaccccggt gggtatgacc gatgcctttc tcactctctt
     1681 cccggtcatc gtcggccttc cccgttattg tggcgtgtca gcaccctcga cggcatttcg
     1741 atggctgctc agtgttcact cacgttacgg cccgcataac ccgttgactt cctgaagaag
     1801 tcgtttcagg gagagattca gcccttttgt tacctcctgg accgcttctt gtccttccag
     1861 tcggagcgag agttagccgg ggcggacttt caccgacgga aagatcgtgc atttcaaggc
     1921 acacacaaca atatctgcga gcgtgctatc cgtccggtcg ttatgggacg aaaggcctgg
     1981 ttgttcgctg gttcactggt ggccgggaac cgcgcggcac agataatgag ccttctggga
     2041 accgcagtct ggagccacat gcttggctga cggacgtcct gacgcgtctg ccggagtggc
     2101 cggaggagag gttggctgag ttactgcctc ttgagggctt taccttcttc gggtaagtga
     2161 tacctgccgt caggtgtttg tgcaccgggc cataacctgc agttgggaat tgaacgcctg
     2221 acaacaggaa atgagctaga gcagtaaaca cgcgggctga caatgggtca gaatatgagt
     2281 tagcccggcg ttattcctgg ggtcagtact tcagatctag cctccggtac cagactagct
     2341 agcccctacg caaccaaata aatcaaccat cgtcagagtg caatcgttca tatacactgt
     2401 cggtgatgcg ctaaaaaatg cgttaatgtt gagttcctcg aacccttacc aattaaggga
     2461 catagccaga tacgcaactg gctgatgcaa gcagtctaat gtctttagat ccgctggcct
     2521 gcgggcatga caatgtcaaa cagtcaaaga ttgtggcgtt gacggtcagt cttggggaca
     2581 aaaaagctca gtattctcgt tggcaaccag ataatttaac agttgatgct gaaacgaaat
     2641 aggcatcttg gt
//
LOCUS       DAZFRE010000236         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFRE010000236 DAZFRE010000000
VERSION     DAZFRE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.242"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..561
                     /locus_tag="ACHKUI_005112"
     CDS             <1..561
                     /locus_tag="ACHKUI_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6601106.1"
                     /translation="SSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPV
                     NWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGV
                     QAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            632..827
                     /locus_tag="ACHKUI_005113"
                     /pseudo
     CDS             632..827
                     /locus_tag="ACHKUI_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            945..1763
                     /locus_tag="ACHKUI_005114"
     CDS             945..1763
                     /locus_tag="ACHKUI_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601107.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRFIEGAYEVVGVFD
                     RIEEKHDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILTPRRREGYGQDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            2105..2578
                     /locus_tag="ACHKUI_005115"
     CDS             2105..2578
                     /locus_tag="ACHKUI_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6601108.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          600 a          743 c          723 g          586 t
ORIGIN      
        1 tcatcggaca ataacgactt ccgcgcccgg ggctggggct ggctgggctc actggaaacc
       61 ggtctgccct tcagtatcac tgacaatctg atgctggagc cacaactgca gtacacctgg
      121 cagggactct ccctggatga cggccaggat aacgccggtt atgtgaagtt cgggcatggc
      181 agtgcacaac atgtgcgtgc cggcttccgt ctgggcagcc acaacgatat gaactttggt
      241 aaaggcacct catcccgtga caccctgcgc gacagtgcca aacacagtgt gcgtgaattg
      301 ccggtgaact ggtgggtaca gccttctgtt atccgcactt tcagctcccg gggtgacatg
      361 agcatgggta cagccgccgc cggcagtaac atgacgttct caccgtcccg gaatggcacg
      421 tcactggacc tgcaggccgg actggaagcc cgtgtccggg aaaatatcac cctgggcgtt
      481 caggccggtt atgcccacag cgtcagcggc agcagcgctg aaggttataa cggtcaggcc
      541 acgctgaatg tgactttttg ataatcaggt cactctcctt caggccccgg tcatcatgac
      601 cggggctttt tactttcagg gagcaacaga tatgccgggt tgtacatctc gtttattgcc
      661 cgaaggccct ttctcacgcg aacaggctgt tgccgtcaaa acagcttacc gcaatgtgtt
      721 tactgaagat gaccagggaa catattcccg gctggttatc cgtaatgctg aaggtcagtt
      781 gcgctggtga tgccggaaca cagcttaata ctcagctcgc cagttaaggg cttctcagac
      841 aataaacgtc attcatttca tctgtcaggc cgcgtcttct ccgggagacg tggctttttc
      901 atttattaca ccagtaaatc gtaaccaccg ataaggagca aattatgcga ctagccagtc
      961 gttttggtta tgcgaaccag atacgccgtg accgtccgct gacacacgaa gaactgatgc
     1021 actatgttcc cagtattttc ggtgaagacc ggcatacttc ccgcagtaaa cgatatgcgt
     1081 acattcccac catcaccgta ctggaaagcc tgcagcggga aggctttcag ccattcttcg
     1141 cctgccagac ccgtgtgcgc gacccgggcc gccggggata cacaaaacac atgctgcgtc
     1201 tgcggcgggc cggagagata aacggagaac atgtccctga aatcattctg ctcaactccc
     1261 atgacggtac ctccagctac cagatgctgc cgggttactt caggttcgtc tgtcagaacg
     1321 gatgcgtctg tggtcagtct ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg
     1381 acagattcat tgaaggggct tacgaggtgg tgggcgtgtt tgaccggata gaggagaagc
     1441 atgatgccat gcagtcgctg gtcctgtcgc caccggcacg ccaggcgctg gcacaggcgg
     1501 cactgactta ccgttatggt gacgaacatc agccagtcac caccgctgac attctgacac
     1561 cacgacgcag ggagggttac ggtcaggacc tgtggagcgc atatcagacc atccaggaga
     1621 atatgctgaa aggcggaatt tccggccgca gtgcaaaagg aaaacgtatc cacacccgtg
     1681 ccattcacaa catcgacacc gatattaagc tcaaccgcgc attgtgggta atggcagaaa
     1741 cgctgctgga gagcctgcgc tgatgcagca tgtccctgaa agcgtgattc aggcaccaga
     1801 atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg atgacctgtg
     1861 cgcctggtgt acccgtctgc tttaccgtcc cggagaaatc agtctgtgca gactgagtat
     1921 acaggcaatg gcatctggcc atccgtctgt gaccggaacg gttacgcgta cggctgtccg
     1981 gaatttcagc cgaatatcat tcatccctga tattaccggg tcatcacggt cagtgatgaa
     2041 cgtgatgttg ccagctcccg acctttactc cctctcattt attcacatta caaggatttc
     2101 atacatgaaa acattgtctc agaacaccac ctcttcggcc tgtgcaccgg agactgacct
     2161 gcaaaaactg gtagccactc ccgtccctga tgaacggcgc atcagcttct ggccgcagca
     2221 ctttggcctc attccacagt gggtgaccct ggagccccgt atcttcggct ggatggaccg
     2281 tctgtgtgaa gactactgcg gtggtatctg gaatctgtac accctgaaca acggcggggc
     2341 atttatggca cccgaaccgg atgacgatga tgacgaaaca tgggtactgt tcaatgccat
     2401 gaacggtaac cgcgctgaaa tgagcccgga agccgccggc attgctgcct gtctgatgac
     2461 gtacagtcat catgcctgtc gtacggagtg ttatgccatg acggtccatt attaccggct
     2521 gcgggattac gccctgcagc atccggaatg cagcgccatt atgcgcatca tcgactgaat
     2581 ggagagaagt acaatgcaac agctttcctt tctgcccgga gagatgacgc ccggcgagcg
     2641 cagcctcatt ca
//
LOCUS       DAZFRE010000237         2596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFRE010000237 DAZFRE010000000
VERSION     DAZFRE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.243"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..260)
                     /locus_tag="ACHKUI_005116"
     CDS             complement(<1..260)
                     /locus_tag="ACHKUI_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6601109.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQI"
     gene            complement(271..969)
                     /locus_tag="ACHKUI_005117"
     CDS             complement(271..969)
                     /locus_tag="ACHKUI_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6601110.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMSGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(969..1310)
                     /locus_tag="ACHKUI_005118"
     CDS             complement(969..1310)
                     /locus_tag="ACHKUI_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601111.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1303..>2596)
                     /locus_tag="ACHKUI_005119"
     CDS             complement(1303..>2596)
                     /locus_tag="ACHKUI_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6601112.1"
                     /translation="ARLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKPKSRG
                     GKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRN
                     AALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSF
                     RQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAESDAQRADWQKGLQ
                     EGLSNWVDNASDYASQAAQLATEGISGLVNNITEMLNGNKVEWRNWASSVLQEISKVL
                     MNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTP
                     TYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQND
                     GANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          506 a          894 c          599 g          597 t
ORIGIN      
        1 tttatctgca gccgccggtc cgcctcgctc agccagggca gaccaccggg atgactgtgg
       61 accagcgcca caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcgatacga
      121 aaatacgcct ccggctctgc ggaaatattc acacaaggga tataccgctc cccctccggc
      181 gtgcttatca cgaagccgca cgactccgca ggcgcacacc gccgggcatg cgccagaatc
      241 gctgattcag tctgtgtcat aaaccgggat ttactgcgaa agtttattaa tggaaaggaa
      301 accgccaaaa ttgccgacat tcctgcgcag ttcacacccg cgcatgcact tgctgcatct
      361 gtccttacgg atatccgtgg tgggtttatc gaactcatcc gccactgccc cgcccgtgta
      421 accacactca tcagagcggt aggtccacat acaggtgttc gccagcatga tacgaccggg
      481 aaacagcgcc ccgtccgtct cggtcggtgt ggccagcaca aacgaggccg tcatggctgt
      541 cagctgcgac atctgctcca ccacccagcg gtcactcagc tcctgctccg ggtccgcctc
      601 cggattgccc gcaacgaaat tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac
      661 caccgtggcc cccaccagac tctgcaggtc ttccgccatc ccggtgacca gaccgaacag
      721 attggacacc gtcagcgacg gtctggcact gctgccccgg ccgctcatct caaaaccgct
      781 gccgtcaatc gggtatgcct catacttacg cccctgccag gtgaccggct cccctttttc
      841 attcagctca ttacagaaaa aataccgctc accgccctgt accgtcaggt cgatttccca
      901 gagtaccacc cgcggtgact gctctgattt aaccgactcg ttcagacttt cttcgtgaat
      961 atcctgcatc agttcaccac ctgctcaatc gtacaactga aatcactgta cctggcgtta
     1021 tctgtgacgc tccactcccg gcataccacc ctcaccgtcc ggttatgttt cggcggtcgc
     1081 cacaaaaagg cacggtaacc accatgccag gataaaaatt catccagcca gcgccgggtt
     1141 ggttcatccg tcacccggaa caccgcctga aacgtcttca gtctggcatt aagtcccgtc
     1201 ggtcggcgct gttcataacc gtcaccaaac cgaaccctca ccaccgacgg tttctcactc
     1261 acctgcatcc cttcacgcgg gaccagatgc agcgttttta tctcagccac tcagcattcc
     1321 tccgtcacgt cgcatggaca gcatcaccgc ctgcacccgc tggtcaatca gctgcacaag
     1381 actgcctgcc gcctccggcc ctatctgtcc gttagccccg tcattctgaa tggcgatgtg
     1441 gtagaccggg gaatacacca gaccggcact gccgttcata ctgcccaccg cgcgtacgcc
     1501 cagcgagcca tccgccgccc gggtcagggg cataatggct tcaggtccgg cttcccccat
     1561 cagcccggcc ccttttgcaa acgcaaagta cgtgggcgtg tccacaatgc tgttgctgta
     1621 cgcactcagg tttgccgagg tatacacgcc gccttttgca ttggccaccg caccgcccag
     1681 ccagtcacca atgctgccaa taaatcctcc cgcaccggac ataccgtttg ccgccgtctt
     1741 aattccgttg acaatcgcgg cattcataag aacttttgag atttcctgca gtacggatga
     1801 ggcccagttg cgccattcca ctttgtttcc gttcagcatc tccgtgatgt tattcaccag
     1861 tcctgaaatc ccctccgttg ccagctgtgc tgcctgagag gcgtaatcgg atgcattgtc
     1921 cacccagtta ctgagcccct cctgcaagcc tttctgccag tccgcacgct gcgcatccga
     1981 ttcggcataa aaggctgcct ggtccttaag gcgttcgctc agatactgcg cgttctgtgc
     2041 ccgtgcctgt ctgtaaaaat cctcactgat atccccggtc tgatactgag actgaaggtc
     2101 cgcatccttc tggcggaagc tgtcgcggat ctgctgcaac tcccgcatgc gttccctggc
     2161 tcgttctccc tgcccgtacc ccagcagttc ggcttcattt gatgcacgcg cagccacatt
     2221 atcattcttc agggtctctt cccgggatcg caactgttcc cggatttttt gctggtcaat
     2281 cagggccgcg ttacgcagca gttcctgctt ctgcatctcc gtcagggttt tcagttctcc
     2341 ctgcgcagtc tggtacttca gcttcgccag ttctgtattc tgacccgcca gtgccagttg
     2401 ctctttctgc tgtttcagta gccgggaaaa actgtcttcc gctttttccg tctctgattt
     2461 tccaccccgg gatttaggtt tgttcgcctc gttattacgc caggcttcca gagcattact
     2521 gatataacgc tgtctcgcct cctgatacga atcccccaca aaaccgaggt catcggccgc
     2581 ataccccagc cgggca
//
LOCUS       DAZFRE010000238         2547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFRE010000238 DAZFRE010000000
VERSION     DAZFRE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.244"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..185
                     /locus_tag="ACHKUI_005120"
     CDS             <1..185
                     /locus_tag="ACHKUI_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601113.1"
                     /translation="SGRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKR
                     RRLRYSTVGLTRYRTR"
     gene            261..944
                     /locus_tag="ACHKUI_005121"
     CDS             261..944
                     /locus_tag="ACHKUI_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6601114.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            945..1166
                     /locus_tag="ACHKUI_005122"
     CDS             945..1166
                     /locus_tag="ACHKUI_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601115.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            1096..1614
                     /locus_tag="ACHKUI_005123"
     CDS             1096..1614
                     /locus_tag="ACHKUI_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6601116.1"
                     /translation="MAPIACSWKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTREND"
     gene            1659..2429
                     /locus_tag="ACHKUI_005124"
     CDS             1659..2429
                     /locus_tag="ACHKUI_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601117.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAEVVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
BASE COUNT          536 a          726 c          771 g          514 t
ORIGIN      
        1 cgtccggccg ggcacgggac gggcgctttt accgctcccg gctggcgtcg gtgacagtgt
       61 acgccagccc gtcgcccttt tctgatgaac gcccttcaag ccgctttcgc ggcatagcct
      121 tgccgtcaaa aagacgacgg ctgcggtatt ccacggtcgg cctgacccgt taccggacgc
      181 ggtgaacagc ccacaggcag cggggaacgg gcaccacagg ggtgccctcc cggtgccctc
      241 tgtaagagaa ggagtttggt atgtcccgtt ttgtcctcgg taactgcatc gatgttatgg
      301 cccgtatccc tgataacgcc attgatttca tcctcaccga cccgccgtac ctcgtcggtt
      361 ttcgtgaccg tcaggggcgt accattgccg gcgataaaac cgatgaatgg ctgcaaccgg
      421 cctgtaatga gatgtaccgc gtactcaaaa aagacgcgtt aatggtgagc ttctatggct
      481 ggaatcgcgt cgatcgtttt atgtctgcct ggaaaaatgc gggattcagc gttgttggtc
      541 acctggtctt caccaaaaac tacacatcga aggccgcata tgtgggctat cgccacgaat
      601 gtgcctacat cctggcaaaa ggccgtccac gtctgccaca aaacccgctg ccggacgtgc
      661 tgggctggaa atattcgggc aatcgccatc acccgacgga aaagcccgtt accagcctgc
      721 aaccactgat tgagagcttc acacatccga acgcgattgt gctggacccg tttgcaggca
      781 gcggatcaac ctgcgtggcc gctcttcagt ccggacgccg gtatatcggt atcgagctgc
      841 ttgaacagta tcaccgtgcc ggacagcaac gactgaccgc cgtgcaacgg gccatgcagc
      901 agggggccgc gaatgataac tggtttgaac cggaggctgc gtaaatgaac tatgccggac
      961 acgaaaaact tcgcgccgac gtggcggagg tggccaacac catgtgtgac cttcgggcca
     1021 ggctgaacga tatggagcat cgctgtcgct ttgattccga tgtgcttgtt gaacgcctga
     1081 cacgccagac gctgtatcgc gccaatcgcc tgttcatgga agcgtatacc gaaattctgg
     1141 aactggattc ctgcttcaaa gattgagaag gagacgagaa tgtacggaac atgcgaaacg
     1201 ctctgccggg cgctggcagc aaagtatccg ggagatacgc cgctgatgct ggttatctgg
     1261 tccccggaag aaattcaggc cctcgctgac ggaatggata tttccctgtc cgatcatgaa
     1321 atcaggactg tcctggcgcg cctggaggac atcccggaag accagcggac tgaatccggt
     1381 atttcttccg gtgcggcgat ggagatcatc aataacgtga gagaaaaccg ccaggtgacc
     1441 gtccctgctg aactgctggc gtccctgatt cagaccgccg aacaggcact gtggaaacgt
     1501 gagtgggccg cccgtgataa cggcctcgcc gtcccggaat gcgtcacccg ccgacaggcg
     1561 gtggttaatc aggcccgcgc cctgctgaaa aacaacacac gcgaaaacga ctgatgttat
     1621 cgccgcctgc gggcggcgat tcagggagac catgaatcat gaacgaaacg ctgaatgcac
     1681 tgatttgtcg ccacgcccgt aacctgctgc tggcgcaggg ctggccggaa gagacggatg
     1741 ttgaccagcg taacccgaag tatccgggct ggatcagcat ttatgttctg ctggatgcgc
     1801 cccggctggc gacgttactc gttaaccgcc acgatggcgt gctgccgccg cacctggcct
     1861 ccgccattca gaaactgacc ggaaccgggg cggaagttgt actgtccggc agtcagtggc
     1921 agtcgctgcc ggtacttccg gcagacggaa cgcaggtgtc tttcccgtat gccggagaat
     1981 ggctgaccga agacgaaatc agggcagttc ttgatgcggt gcacgatgcg gtacgcagtg
     2041 tcagttgccg ggtggcagaa gatgcgcggc gtatcagggc ggcgctgacc acaaccggtc
     2101 agacgttgct gacccgccag acgcgccgct ttcgcctggt tgtgaaggaa agcgatcacc
     2161 cctgctggct cgatgaagac gacgaaaacc tgcccgtggt gctcgatgcc atcctgaacc
     2221 ggggcgctcg tttttcgtcg gtggaaatgt atctggtcag tgagtgtgtg gagcatatcc
     2281 tgtccagtgg gctggcctgc gatgtgctgc gtatacctga tgaaccgtcc cgtcgctggt
     2341 ttgaccggga tattctgcgg gaggtggtcc tggaagcccg gactgaaatc cgcagcatgg
     2401 cggatgcact ggcgaaaatc cggaaatgat gacgctggcg gggaaaaatc tccgcctttt
     2461 ccagccggtc cccggaaaga gcaaaaggat gtcgccgcag gctccgcttt acccggccat
     2521 gcggggcatg gccttgtggg ttttcag
//
LOCUS       DAZFRE010000239         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFRE010000239 DAZFRE010000000
VERSION     DAZFRE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.245"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..163
                     /locus_tag="ACHKUI_005125"
     CDS             <1..163
                     /locus_tag="ACHKUI_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO6601118.1"
                     /translation="PGKMKCIRVVTADEHLKEVYTEDASLPGKIRKCPVYLPDDRTNR
                     NNGDKNEHA"
     gene            150..470
                     /locus_tag="ACHKUI_005126"
     CDS             150..470
                     /locus_tag="ACHKUI_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO6601119.1"
                     /translation="MNMRNINAITALSVPGKTMSDDFMHAVLSNCTTRIVLPAPKEFS
                     SESLPHNFNMAAVGVMKEGMSMRLFSSPGHIVVAGQFGGGKSASVKELIDRIIQKYKE
                     PFDE"
     gene            463..715
                     /gene="trbG"
                     /locus_tag="ACHKUI_005127"
                     /pseudo
     CDS             463..715
                     /gene="trbG"
                     /locus_tag="ACHKUI_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
     gene            712..1227
                     /gene="traV"
                     /locus_tag="ACHKUI_005128"
     CDS             712..1227
                     /gene="traV"
                     /locus_tag="ACHKUI_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO6601120.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQPADAKPAAASLPRLAEGNFRTTTVRAVTAASPSGSGPVVTALPEQKLLAPR
                     PLFTAAREVKTIIPVSSVASVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            1362..1583
                     /gene="traR"
                     /locus_tag="ACHKUI_005129"
     CDS             1362..1583
                     /gene="traR"
                     /locus_tag="ACHKUI_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO6601121.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            1576..2049
                     /locus_tag="ACHKUI_005130"
     CDS             1576..2049
                     /locus_tag="ACHKUI_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601122.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            2129..2347
                     /locus_tag="ACHKUI_005131"
     CDS             2129..2347
                     /locus_tag="ACHKUI_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601123.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIESHGGRG
                     FGNTGECIEVKTNKPLNNVMDTLNGGGQ"
BASE COUNT          743 a          501 c          646 g          656 t
ORIGIN      
        1 acccggaaaa atgaaatgta tccgggtggt aaccgcagat gaacatctga aggaagttta
       61 tacggaagac gcttccctgc cgggaaaaat caggaaatgc cctgtttatc ttcctgatga
      121 cagaacaaac agaaacaacg gagataaaaa tgaacatgcg taatattaat gctattacag
      181 ccctttctgt accgggtaaa actatgtcag atgattttat gcatgctgtt ctcagtaact
      241 gcaccacaag aatcgtcctt cctgcaccga aggaattcag ttctgaatca ttgccgcaca
      301 attttaatat ggctgctgtc ggtgtgatga aggagggaat gtcgatgcgt ttattttctt
      361 ctcctgggca tattgtggtt gccggacaat tcggtggcgg aaaatctgct tcggtgaaag
      421 aactaatcga cagaattatt cagaaatata aggaaccctt cgatgaataa gctggttgct
      481 gatggcaacg tcaaaaaaaa taaattatcc ggtgctgtat gagtccggaa taacacctcc
      541 gttatgtgaa gtcagtgcac cagaacctga tgcgggtggg aaacgcattg tggcatatgt
      601 ttataaatca tcacggaata cggtttttga aaatcctgac attgtaaaaa cctgtacggt
      661 cagggatttg aagaatgatt ttgtgaactg cgatgagaaa ggtgagggac aatgaaacag
      721 atttctttat ttattcctct gctggggacc ttattacttt ccggctgtgc cggaaccagt
      781 acggaatttg agtgtaacgc caccacatcc gatacctgta tgacgatgga gcaggccaat
      841 gagaaggcca aaaagctgga gcagcctgca gatgcaaagc cggctgcggc atcactgccg
      901 cgcctggctg aagggaactt ccggacaacc acggtgcggg ccgtcaccgc ggccagccca
      961 tctggcagcg ggcctgtagt gactgcactt ccggagcaga aactgctggc accacgtccg
     1021 ttgtttacgg ctgcccggga agtgaaaacg attattccgg tcagttcagt tgcgtcggta
     1081 acacctcccc gcccgctaag aacgggtgag cagacggcag cattatggat agcgccttat
     1141 attgataatc aggatgttta tcatcaacca tccagtgtgt tttttgttat taaaccgtct
     1201 gcgtggggaa aaccacgtat taattaactg gccctgaagt tgtatatcag ccggcaatgc
     1261 cggcgggctt tctgtattca aaatctgcct gtaaaacgag tgccctgtgc gtgaaaaggg
     1321 atgggcggta gcgtgtcaga acatgtcggg aggaatattc cgtgagtgat gaagccgatg
     1381 aagcatattc agtgacagaa caactgacca tgacaggaat aaaccgtata cgccagaaaa
     1441 taaatgttca tggtattcct gtttatctct gtgaggcatg cggaaatcct attccggaag
     1501 cccggcggaa aatatttccc ggtgtgacgt tatgtgttga atgtcaggcg tatcaggaaa
     1561 aacagagaaa acactatgca taaatcagaa gctgaaaaaa tagattctat tctggatgaa
     1621 catcaacaga tatatcgcag acataaccaa ataaataaca tcctggcgta ttgtgcgtgt
     1681 attgcatgga ttccctgtat tattgctgcg ttcggcgttg atggtatata tctgaaaatc
     1741 ctgtttgcgg tccttgcctg ctgtggtgcg gtatgctttt ttattttctt cttcactctc
     1801 ctgccggaat cactcatggt cctcagccgt cagagcctgc ttcagcttat gcgtctgact
     1861 gaagacgtac cggatgcgag acaggaactg cttaaccgtt tgttgtccgg taaaaaactg
     1921 actggtcggg atgaacagga tatccggcgt ctctggcagg agaaagtgga tgcaatgcag
     1981 gagtctgcca cccgtcagcg tgaacaggat actattcgga agtttactga agggaataaa
     2041 tccgaatgag aatctgcagt gttttatgac ctcataagtt aactgaaggg atattcagtg
     2101 attaatatac aagcggagat aataaagaat gcataccttt atttttcttg tgctgataaa
     2161 aaatattaac ggtggcggaa gctggaatat tacacccatg ccaaatacgg acgtatgcgt
     2221 gcaggttctt cggacaatcg agtcacacgg aggcagggga tttggaaata caggggaatg
     2281 tattgaagtt aaaacaaata aacctttgaa caacgtgatg gatacactga atggtggtgg
     2341 gcaatgagtg cttactgctt caaaggaaaa tgaaatgaag tcgatatatt tagaatctgt
     2401 tctggctttt atatttgttg gcgttatggc tatgtttaag tcttggtaag gcaaaagagc
     2461 ttgcatcagc gtgtagagaa agaaacgaaa tggcagaggt gaaacgggta agagaaaatg
     2521 agcgaaataa gatacgtgaa aaacaa
//
LOCUS       DAZFRE010000240         2472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFRE010000240 DAZFRE010000000
VERSION     DAZFRE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.246"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..2051
                     /locus_tag="ACHKUI_005132"
     CDS             <1..2051
                     /locus_tag="ACHKUI_005132"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFO6601124.1"
                     /translation="KSRKSVKSVEPEITELANSWLKSYRLDYKLQNASLNGEIDNALD
                     AYFSKNGGAGGNRPDVKLLVKDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLEDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2120..>2472
                     /locus_tag="ACHKUI_005133"
     CDS             2120..>2472
                     /locus_tag="ACHKUI_005133"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6601125.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEY"
BASE COUNT          763 a          453 c          601 g          655 t
ORIGIN      
        1 gtaaatcaag aaagtctgta aaatctgttg agccggaaat taccgaactg gcgaactcat
       61 ggcttaaatc ttacaggctt gattacaagt tgcagaatgc atcacttaac ggagagatcg
      121 ataacgcact ggacgcttat ttttcgaaaa atggcggcgc aggcggtaat cgtcccgacg
      181 taaaactcct tgttaaggac aaatacggca agcagtatcc cgttctcatc gagtataaag
      241 gctacaagga caggcttatc agacttggca gcaacggtat tattgagaac aaagatgcca
      301 gaaaagaatg gaattttaaa aatattaatg gatacgcggt gaatggcgca gtccattatg
      361 ccaacgcact tcttcagttc accaattacc ctgatattat tgctattggt atgaccggct
      421 ggcgcgatga tgatacaggc gaactgcatc atgaaatcgg cgtgtggtac gtatcaaaga
      481 ataatttagg agccggacag aaggttggtg aattcaccga cttatcgttc cttgctgaca
      541 aaaatgttga tggatttctc aacaagataa agctgcttaa tcttcctccc gaagaactgg
      601 aaaaaatcaa ggccagcaaa gaggaagaga tagacacgcg actttcccgt ctgaataacg
      661 atatttacca gaatgaaaag gggcttggtg aaagcgatcg tgtttacctt gttgtggcta
      721 ccgttattgc aacgttgggg attccgggaa agttagcacc gctggataaa aaagaactga
      781 cctcttcaac ggaggaggac ttgcgtgatg gtgatattat tttcaggaaa ataaggaatt
      841 ttttaaggct gaaagctgtt cctgaaacaa aaagagaaat gattttgcgc tcgttgcaaa
      901 atacgctgtg gactgaaaac attaacaaac cagtcaacgg tgaaagccag ttaaagcgcg
      961 tgtttgttaa agttgttgat gatcttgggg agtattacaa aattggactg accaccgatt
     1021 ttacgggcaa actgtttaat gagatgtatc gctggcttgg atttacgcag gacaagctca
     1081 atgacgtcgt tctgacacca ccttatgttg ccacattgct ggcacgactc gccagagtaa
     1141 acaaagattc ctatgtgtgg gattttgcta caggttctgc gggattgctt gtggctgcaa
     1201 tgaatgaaat gctcattgac gccagagaaa atattcactc accgaacgaa ctacaactta
     1261 aggaagcgca aatcaaggct gaacagcttc ttggccttga ggtgttatcc agtatttata
     1321 tgctggccat cctgaatatg attttgatgg gggatggtag ctcgaatatc ctcaataaag
     1381 actcgctggc cgattttgac ggcaagtatg gatttgggaa aacaggagaa aaattccctg
     1441 cggacgcatt tattctcaat cctccgtatt cagctaaagg taacggcatg attttcgtgc
     1501 agaaggcgct gtcgatgatg gataaaggct atgctgcagt gattatccag agttcagctg
     1561 gcacaggaaa ggctacggag tacaacaaaa aaatactcaa ggaaaatacc ttgctggcaa
     1621 gcatcaaaat gcctgcggat ctgtttattg gtaaatcaag cgttcagacg tacatttacg
     1681 tttttcaggt aaaaattccg cataacgcga aacaggctgt taagtttatc gacttttcca
     1741 atgacggcta cgctcgctct aatcgcaaaa aggcaagaaa caatcttgtt gatgctgatc
     1801 gtgcaaaaga gcgttatcag gaggttgtgg atctggttca cttcgggaaa ggttgcctta
     1861 atatctttac ggaggatgaa tattttgagg gaacaatcga tcctgatagt ggtgaggact
     1921 ggaatcagac caggccagta gatgctaggc caacgctgga ggatttcaaa aagacggtag
     1981 gtgattacct tgcatgggag gtctcgcaat tactgaaaaa acaaggtgaa aacaattttg
     2041 cgggaaagta aattccccgc tcagcgataa attaagaagc gttgagtggg gagagttcag
     2101 gattggcgat ttgttctatg tgagcacagg ctcgcttatc tcctcaggaa gattaaaacc
     2161 aggcaaaatc aaacgtgttt cagcaaagag cgatcaaaac ggtatcatcg gagagtttga
     2221 taccgagttt atggatgatg caaggcatta tgaaaatttt ataagcgtta atttttttgg
     2281 agatgctttt tatcatcctt attgtgccag cgtagaaatg aaggtgcatg ttcttacatt
     2341 aaaatcaagt aagtccgact ttaatgcaaa gcgcggactg tatgttgcca gtatgattaa
     2401 taaagcttta aaggggcgtt ttaattatgg tcaacagtta tcgagttcca aactcagaga
     2461 tggagaatat tt
//
LOCUS       DAZFRE010000241         2442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFRE010000241 DAZFRE010000000
VERSION     DAZFRE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.247"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            182..685
                     /locus_tag="ACHKUI_005134"
     CDS             182..685
                     /locus_tag="ACHKUI_005134"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6601126.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(731..1147)
                     /gene="rraB"
                     /locus_tag="ACHKUI_005135"
     CDS             complement(731..1147)
                     /gene="rraB"
                     /locus_tag="ACHKUI_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6601127.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1309..2313
                     /gene="argF"
                     /locus_tag="ACHKUI_005136"
     CDS             1309..2313
                     /gene="argF"
                     /locus_tag="ACHKUI_005136"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6601128.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          633 a          563 c          600 g          646 t
ORIGIN      
        1 gaattaaaaa atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt
       61 acaatactct tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc
      121 acgtaagaag agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac
      181 tatgaataac attgcgccgc aatcacctgt aatgcgtcgc ctgacgttgc aggataatcc
      241 tgctatcgcc cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg
      301 ctacaccgtc gccgatccga atcttgacga gctgtatcaa gtgtatagtc agccgggtca
      361 tgcatattgg gtggttgagt acgagggtga agtggtcggc ggcggtggga ttgcgccatt
      421 agcaggtagt gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg
      481 cggtaaaggg ctggcgaaaa aactggcctt aatggcgatg gagcaggcac gagaaatggg
      541 cttcaaacgc tgctatctgg aaacgaccgc ttttttaaag gaagccattg cgctttatga
      601 gcatttgggc tttgagcata tcgactatgc gcttggctgc acgggccatg tcgattgtga
      661 agtgcggatg ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt
      721 aatcaattaa ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc
      781 cgtcttcgcc gttgggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt
      841 tctccgccag cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc
      901 actcgctgag gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg
      961 gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca
     1021 gatcgtctgc ggaaagatgg tgttcgatgg tgtacagtgc gtccggatcg ctgccatctt
     1081 ccagtaattc ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt
     1141 ttgccatggc tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc
     1201 attgcctcca cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca
     1261 tataaattga attttaattc attgaggcgt tagccacagg agggatctat gtccgggttt
     1321 tatcataagc atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg
     1381 cagttagccg cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact
     1441 ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa
     1501 gttgccgcat atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt
     1561 ggtcataaag agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt
     1621 cagtatcgcg gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg
     1681 gtatggaacg gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc
     1741 atgcaggagc atttacccgg taaagcattc aacgaaatga cgctggtcta tgcaggtgac
     1801 gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg
     1861 cgtctggtcg cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgct
     1921 ctggcacagc aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggagttgaa
     1981 ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agagaaatgg
     2041 gctgaacgca ttgcattgct gcgtgattat caagtgaaca gcaagatgat gcagttgacc
     2101 ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga ccaaacgacg
     2161 cttggcaaga aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag
     2221 gtcttcgaat ctgccgccag cattgtattt gatcaggcgg aaaaccgcat gcataccatc
     2281 aaagcggtga tggtcgcgac gctcagtaaa taattgatat tgttataaag atgccgggaa
     2341 atacaaacca tcccggcatt acattatttt atgcagtgat aggtttatta ttccacccaa
     2401 atttttctat tgctatacgt aaattttccg gtggatttag tt
//
LOCUS       DAZFRE010000242         2440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFRE010000242 DAZFRE010000000
VERSION     DAZFRE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.248"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            37..546
                     /gene="eco"
                     /locus_tag="ACHKUI_005137"
     CDS             37..546
                     /gene="eco"
                     /locus_tag="ACHKUI_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6601129.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(686..2332)
                     /gene="mqo"
                     /locus_tag="ACHKUI_005138"
     CDS             complement(686..2332)
                     /gene="mqo"
                     /locus_tag="ACHKUI_005138"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6601130.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMIHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAVDSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          585 a          663 c          602 g          590 t
ORIGIN      
        1 ttaaccttca gcgacatcat cggtgaaaac ctataaatga agaaggaaag caaaaaaatg
       61 aagaccattc tacctgcagt attgtttgcc gctttcgcca ccacttccgc ctgggcggca
      121 gaaagcgtcc agccactgga aaaaatcgca ccttatccac aagctgaaaa aggcatgaag
      181 cgtcaggtga ttcagttaac tccgcaagaa gatgaatcta ccctgaaagt agaactgtta
      241 atcggtcaga cgctggaagt cgactgcaat ttgcatcgtc tcggcgggaa gctggaaagc
      301 aaaacgctgg aaggctgggg ctatgattat tatgtctttg ataaagttag ctcgccggtt
      361 tccacgatga tggcctgccc ggatggcaag aaagagaaga aatttgtcac cgcgtatctg
      421 ggcgatgctg gaatgctgcg ttacaacagc aagctgccga tcgtggtgta tacgccagac
      481 aatgtggatg tgaagtaccg tgtctggaag gcggaagaga aaattgacaa cgcggttgtt
      541 cgctaaactg ctgtgaagtg ctgcaacctg taggccgaat aaggcggtta cgctgcatcc
      601 gacatctaac gcccgagccg gttgcctgat ccgacgctga aagcatacgc cacatccggc
      661 aaaccatgcc ggatgtggca catcattaca acgcaatatc cgccacttct ttttgaacgg
      721 gttgcggttt caactgcggt ttcggcgtac tatctactgc ctgcggcttg tcgtatttca
      781 gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca tcaccgttca
      841 gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc gggctggaaa
      901 cgcgatcgcc aaatactttt tccagcagat tcaacataat cggcgcggcg gttgacgccc
      961 ctggcgatgc ccccaggagc gcggcaatgg tcccttgctg gtcactgacg acttcagtac
     1021 ccagacgcag tacgccacct ttatcggcat cacgcttgat aatctgcacg cgctgccccg
     1081 cttgccacaa acgccagtcc tcttttttcg cctgcggata gtactctttc aacgcttcaa
     1141 aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga tcgaaattat
     1201 ccagcccaac gtgtatcatc ggcatcacgt tagaggtggt ggtggaactc atcagatccc
     1261 acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca aacagcacta
     1321 cgcgtttacc gtcaagaaca cgggtatcga tatgcggaac cgacatcggt ggtgcgccaa
     1381 cggatgcttt accgtaaacc ttcgccagat ggtgattaac cacgtccggg ttttccgaaa
     1441 caaggaactg tccacccacc gggaaaccgg cgtagtcttt cgcctccgga atccccgatt
     1501 cctgtaacag tttcagcgcc gcaccgcccg cgccgataaa gacaaatttg gcgcggatgt
     1561 tctgtgcagt gccgtttttc agatcggcaa cggtgacggt ccagctgtta tcgtcattac
     1621 gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc ttctgtaagg
     1681 aggcgatcaa ctggcgggtg atctcgccat agttcacatc agtgccaatt tctgtgcgtg
     1741 ttgccgctac tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
     1801 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
     1861 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
     1921 cggtattgat aaatgaacgc ggagtacgca gcacaccgcg ctcgacctgg tgcgcccaga
     1981 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
     2041 cgtcggcatt ttgcggggtg taattgagtt ccatcagtgc agaatgcccg gttccggcgt
     2101 tgttccagcc gttcgaactc tcctgcgcga caccttccag gcgctccacc atggtcatcg
     2161 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
     2221 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
     2281 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
     2341 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
     2401 aagtaacaac atttaaataa tgtttataaa ttatattcta
//
LOCUS       DAZFRE010000243         2415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFRE010000243 DAZFRE010000000
VERSION     DAZFRE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.249"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(34..492)
                     /locus_tag="ACHKUI_005139"
     CDS             complement(34..492)
                     /locus_tag="ACHKUI_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601131.1"
                     /translation="MAPDTTTGRDPPQRREARFAKRRCALHPGGLLPGCQELRPRRDR
                     GGATEAARPERSLQAGGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDS
                     NQSSRKKKNSSSSSSSSSSRNNNIISNRQMTGLKRQKPPSERPRNERGGA"
     gene            794..1321
                     /locus_tag="ACHKUI_005140"
     CDS             794..1321
                     /locus_tag="ACHKUI_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6601132.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQPQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYWFSDDIPF"
     gene            1378..1611
                     /locus_tag="ACHKUI_005141"
     CDS             1378..1611
                     /locus_tag="ACHKUI_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6601133.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1670..>2415
                     /locus_tag="ACHKUI_005142"
     CDS             1670..>2415
                     /locus_tag="ACHKUI_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6601134.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFR"
BASE COUNT          533 a          663 c          723 g          496 t
ORIGIN      
        1 accgcgcgtc ggtggcgctg aaaggggccg cgctcacgcg ccgccccttt cattacgcgg
       61 acgttctgat gggggcttct gtcttttcag acctgtcatt tgtctgttac tgatgatgtt
      121 gttgttcctg ctactgctac tgctactgct actgctactg ttcttctttt ttctactgct
      181 ctgattactg tctgttttgt tgttctcctg cttatgcctt ttccgggcgt ccccctggcg
      241 gggcgggctg tcgtgaacgg agccagcgga gctgtctccg ccctggcggg cttccatccc
      301 tgacgcaaaa cccccggctt gcagcgaccg ttccggtcgc gccgcctctg tcgcgccgcc
      361 tctgtcgcgc cgtggcctga gctcctggca tcccggcaaa aggccgccgg gatgtaaggc
      421 gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc gggtccctgc ccgttgttgt
      481 gtccggtgcc attcacctgt ttaagcccct gtcgccgggg gctgatacag ccacaacctg
      541 ccgcactgac tgtccagggt aaatgaacag cccacaaaat tttttaccca aaacccggta
      601 aaaaattttg cgttctgccc aagacaggtg cgtcaggccg tggcagtgat gccccttgcg
      661 gcgaaggttc tcaaacaggt gatggtgaat accggaggga acaaaaggca ggtccccgca
      721 aaacgtcaaa accgcggctc ccgaaactca acagatgatg ggggctgaaa acatcaacag
      781 aaggagacac atcatggcag ttcgtggaat taacaaggtc attcttgtcg gtcgtctggg
      841 aaaagatccg gaagtccgtt acatccccaa cgggggcgca gtggcaaacc tgcaggtcgc
      901 cacgtcagaa acctggcgcg acaaacagac gggggaaatg aaggaacaga cggaatggca
      961 ccgcgtggtg ctgttcggca agctcgcgga agtggcaggt gaatatctgc gtaagggcgc
     1021 gcaggtctac atcgaggggc aactccgtac ccgtagctgg gaagataacg gtattacccg
     1081 ttacgtcact gaaatccttg tgaaaaccac gggcaccatg cagatgctgg ggagtgcacc
     1141 acagcagaac gctcaggtgc aaccgcagcc tcagcagaac ggccagccac agagtgctga
     1201 cgcaacgaaa aaagggagcg cgaaaacgaa aggccgtgga cgtaaggctg cgcagccgga
     1261 gcctcagccg caaccgccgg agggtgagga ttactggttt tcagacgata tcccgttctg
     1321 aatgactgac tgtgaaaacc gccccgtcca gtgcggggca tcaccggaga gatgaggatg
     1381 agcgaatatt tcagaatact tcaggggctg cctgacggct cctttacccg cgaacaggct
     1441 gaagccgttg ccgcacagta ccggaatgtc tttatcgagg atgatcaggg aacgcatttt
     1501 cgcctggttg tccgcaataa cagtggcctg gtctggcgca cctggaattt tgaggacggt
     1561 gccgggtact ggatgaacca tgtcatccgg aatttcggca ttcttaagta agagaggtgc
     1621 cggacgcgca aacgtccggc aggacataag caattataag gggatgatta tgcctgtaac
     1681 gaagtgtgaa ccagaaacca cccgcaaagc aagccgtaaa tatgcaaaaa cgcaggaaac
     1741 agtcctgtct gccctgctgg cgcagacgga ggaagtgagt gtgccgctgg cctcactgat
     1801 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgcg gagtccgtca gcgaactggc
     1861 tgagtccatt aagggcgttg gcctgttgca gaatctggtc gtgcatactc tgcctggtga
     1921 ccgttacggt gtcgccgcag gtggtcgccg actggctgca ctcaacatgc tggcagagcg
     1981 tggcatcatt ccggctgact ggcctgtccg tgtgaaggtc attccgcagg agctggcgac
     2041 ggcggcatca atgactgaaa acggtcatcg tcgggatatg caccctgctg aacagattgc
     2101 cggattccgc gcaatggcgc aggaaggcaa aacacctgca caaatcggtg atttgctggg
     2161 ctattcaccc cgccacgttc agcgaatgct gaaactggct gaccttgcgc ctgtcatcct
     2221 cgatgcgctg gcagaagacc gcatcaccac cgaacactgt caggcgctgg cgctggagaa
     2281 cgacaccgcg cgtcaggtgc aggtgtttga agctgcctgt cagtcgggat ggggcggaaa
     2341 accggatgtg cgggttatcc gcaatctgat tacggaaagt gaagtggcgg tggcggggaa
     2401 cagtaaattc cgctt
//
LOCUS       DAZFRE010000244         2412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFRE010000244 DAZFRE010000000
VERSION     DAZFRE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.250"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..456
                     /locus_tag="ACHKUI_005143"
     CDS             <1..456
                     /locus_tag="ACHKUI_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6601135.1"
                     /translation="ARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAG
                     ASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRVTDNGRQNTYF
                     SSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            794..>2412
                     /locus_tag="ACHKUI_005144"
     CDS             794..>2412
                     /locus_tag="ACHKUI_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6601136.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPASVRLLIQLQRNGRWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLD
                     REVTLPETGTATVNLINGSGKPASVAITAHPA"
BASE COUNT          556 a          620 c          771 g          465 t
ORIGIN      
        1 gcccgtcttc acgaacagct gggtgaggga acggtcatcc acattgtgcc gcgactggcc
       61 ggagccggaa agggcggact gcagattgtg ctgggggcgg cagccatcgt gggctctttc
      121 ttcacggccg gtgcctcgat ggcgttatgg ggttcagccc tggcagccgg tggtttttct
      181 gccaccacga tgctgttttc actgggggcc agcatgatac tgggcggtgt ggcacagatg
      241 ctggccccga aggcaaaaac accggattac cgcgtaacgg ataacggcag acagaacacg
      301 tacttttcct cgctggataa catgattgcc caggggaacc cgatgccggt gccttacggg
      361 gaaatgctgg ttggctcccg ccgtatatcc caggacatca gcacccgtga tgaaggcggg
      421 ggcggaaagg tcgtggttat cgggcggcag gggtaaaaag aataaaaaaa tcccgcagag
      481 ttgcggagct gcgggagcgt tacgaagatc aactatagat aattattctt atgtcacgac
      541 aaaaaaatta acgcagagaa attatacgcg ccacagtcag tttgtgaaaa tgtgaagata
      601 ttcagaattt ttatgccatt accggtttta accaacagga ttatcggtgg gcatgaaaga
      661 aaaccccggt atctgctgat accggggttt ctctttagca tggcagaaat gtgtttcatg
      721 cttttcgggc gaaggatatc cgacttctgt acggaatggc aagtggcggt taatttattc
      781 aggggaaggc tgtatgggaa aaggtggcgg taaggcacac acgcctcgtg aggcgaagga
      841 taatctcaaa tccacgcaga tgatgagtgt gattgatgcg attggtgagg gaccgataga
      901 aggtccggtg aagggactgc agagtattct ggtgaacaaa accccactga cggacacgga
      961 cggcaatccc gtgatacacg gtgtgacggc ggtctggcgt gccggggagc aggagcagac
     1021 accaccggaa ggctttgagt cctccggagc tgaaaccgga ctgggtgtgg aagtgacgaa
     1081 ggcaaagccg gtgacgcgca ccattacgtc cgcgaacatt gaccgcctgc gggttacctt
     1141 cggggtgcag tcactggtgc agaccacgtc aaagggcgac cgtaacccgg catccgtccg
     1201 cctgctgatt cagttacagc gtaatggccg ctgggtgacg gaaaaggatg tcaccattaa
     1261 cggcaagacc acctcgcagt tcctggcctc ggtgattctg gataatctgc cgccccggcc
     1321 ctttaacatc cggatggtcc gggagacggc ggacagcacc acggaccagt tgcagaacag
     1381 aacgctctgg tcatcgtaca ctgaaatcat cgatgtgaaa cagtgctacc cgaacacggc
     1441 cattgtgggg ctgcaggtgg atgcggagca gttcggcggc cagcagatga cggtgaacta
     1501 ccatatccgc ggtcgcatca ttcaggtgcc gtcaaactat gacccggaaa aacgcacgta
     1561 cagtggtatc tgggacggca gtctgaaacc ggcatacagc aacaatccgg cctggtgtct
     1621 gtgggacatg ctgactcacc cgcgctacgg catgggaaaa cgtctggggg cggcggatgt
     1681 ggacaagtgg gcgctgtatg ccatcgggca gtactgcgac cagacggtcc cggatggttt
     1741 cggggggacc gagccgcgga tgacctttaa tgcgtacctg gcacaacagc gtaaggcgtg
     1801 ggacgttctc agtgatttct gctctgcgat gcgctgtatg ccggtatgga acggtcagac
     1861 gctgacgttc gttcaggacc gcccgtcgga tgtggtgtgg ccgtacacca acagcgatgt
     1921 ggtggtggat gataacggcg tgggattccg ctacagcttc agtgccctga aggaccggca
     1981 cacggcggtg gaggtgaatt acaccgaccc gcagaacggc tggcagacat ccacggaact
     2041 ggtggaagac ccggaagcca tactgcgcta cggacgcaac ctgctgaaga tggacgcgtt
     2101 cggctgtacc agccgggggc aggcgcaccg tgccgggctg tgggtgataa agaccgaact
     2161 gctggaaacg cagacggtgg atttcacgct cgggtctcag gggctgcggc acacacccgg
     2221 tgacatcatt gaaatctgtg ataacgacta tgccgggacc atgaccggcg gacgtgtcct
     2281 gtccatcgat gccgccagcc gtaccctgac gctggaccgg gaggtgacac tgccggagac
     2341 cggcacggcc actgttaatc tgattaacgg cagcggtaag ccggcgagcg tggccatcac
     2401 cgcacacccc gc
//
LOCUS       DAZFRE010000245         2291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFRE010000245 DAZFRE010000000
VERSION     DAZFRE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.251"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..164
                     /locus_tag="ACHKUI_005145"
                     /pseudo
     CDS             1..164
                     /locus_tag="ACHKUI_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            701..973
                     /locus_tag="ACHKUI_005146"
     CDS             701..973
                     /locus_tag="ACHKUI_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFO6601137.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
     gene            1419..1594
                     /locus_tag="ACHKUI_005147"
                     /pseudo
     CDS             1419..1594
                     /locus_tag="ACHKUI_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1820..>2291)
                     /locus_tag="ACHKUI_005148"
     CDS             complement(1820..>2291)
                     /locus_tag="ACHKUI_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601138.1"
                     /translation="NPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLSG
                     ISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKDI
                     IRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
BASE COUNT          701 a          436 c          439 g          715 t
ORIGIN      
        1 ggcaaaaaaa cgatggaaaa tgaactcctt aaagaagccg ttgaatatgg gcgtgcaaaa
       61 aagtggatag cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt
      121 gtctccgggt gtcgcgtgcg cagttgcacg tcattctcag acgaaccgat gactggaaat
      181 ataggggcaa atccacatcc ttacttttcc gtaaggattt tagcgggtca aaacagcgtt
      241 ccatgccgga ctgatgatct acatacttag agtcacatac caaagtaaaa ctatggatgt
      301 atgcaagcct gcatcgtcag gaggttaaat ttcagacctg ccagttaccg cgtaaatggc
      361 ttgatatatg actaatttat ttgcacagga tttttacgac aacgctctga accagcagtt
      421 gacgggatat atagttgagc tcattacaga taaagctttt ctgtatctct ggcggtggtc
      481 atagaccagt aatcgttatt ggttgttcta tattatcccg tatgacggta caacgttttt
      541 aacaactggc gctgtcacaa cataagccac cccaaaatat tatatgagta acaatagtct
      601 atataggagc gtaaattacc tactatcaaa tacttactcc aatagcattt ctgtctattc
      661 tatttcaatt ctttaataga aaaataaggt tagataatat atgactccct ccccctatcc
      721 aagaaataac tttaatacag agctaattca atcatgtatg aatggagaac acttttcact
      781 tttaattgaa atatcaccaa tcagaagtaa aaaaaacatc atggctctta aggagtattt
      841 ggttgatggt tattcaaagc aagaaagttg tgaaaggaac aatgtttcca ttagttattt
      901 ttgcctatgc ctaaaaaaac taaactatac acacagtatt gttagtgctc ttaaatattt
      961 ctaccgtgag taaactgtaa ctgaagtctt gattcttacg cttagacact gctaagccgt
     1021 tatggcatag ctgataattt atattgcact atgtcagcta ctgtaagata atataagcta
     1081 ttttaaagaa aactctgtca ttcatcattt tgtttaatga taggttttgt tattaatata
     1141 aaagaacatg ctcattcatt aaaataaaaa caagatgttc ttttattatt ttcattccct
     1201 acggggttag tccttgattc attttcagta tcttctaact cattcatttg ccgattactt
     1261 taattcacag tcttgttttt tatcctgcct attggagata aaagtggtat cgatggatgc
     1321 cgagtgtgtt gcacatgcag tattctcagg aataagaact gtcacttcca aaacacgcga
     1381 tatatgattg aatctctgtg gggggataaa aacttgcctt tttccgcagt ctgaaaacag
     1441 gatgggggcg acggatggtt acgcaggcaa gggcgaggtt cggaaataaa tcagagatta
     1501 tattctgaat tattacaata gcaccagacc tcaccattac aacggtagtt tgacgctgga
     1561 gggatcagaa aacagatacc gtttttactg taaacggtgg tcatatatgg actctcctgg
     1621 tgatataagt actggttcga aacggtttac gactatatat ccggcgtcat cagtaggttc
     1681 aatgaaccgc gcaataatgt tatccgagcc ctgtttccta atcactacgc cggtatttga
     1741 ttagccaagt caagttcatc ttgccatcac tatttttcca taccgcatta ctatctaata
     1801 cagtcgcacg agcggccttc taatttgagg gtttcgagcc ctgaagaggc atatcccatc
     1861 gttttacaga tattgcagaa attggtacgc aatatgctgc ttcttcctgg agtttcttcc
     1921 tggtatttgt atcgaagtct acattaagca ggtcgaactt ccagctttcc cgataaaacc
     1981 ttataatatc cttgggacaa aggagaataa actggtttgt cgtaaacata gaaacagcct
     2041 gcacagagta atattccccc ggttttatat tttttttacc ccatactgca ttggaaaaaa
     2101 actcctccgg atattcactt gttttaataa tgtagctatg tattttgaag tcacttatac
     2161 ctgagagctt aataactgaa tgggtaaaaa ttactggtaa tacggttatt tgagtcgata
     2221 taactaataa tatcatcaag gatgcgacaa atgcagaact aattctttta gaaagtttat
     2281 gctgtggatt c
//
LOCUS       DAZFRE010000246         2218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFRE010000246 DAZFRE010000000
VERSION     DAZFRE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.252"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1346
                     /locus_tag="ACHKUI_005149"
     CDS             <1..1346
                     /locus_tag="ACHKUI_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601139.1"
                     /translation="RYFNNGKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDG
                     KLMQENFYQQSELIGKDTSYYQSGKINSIHNRNSRGQYDGINERYSEEGKLLSKSVYK
                     DGKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGDSEE
                     WYENGIPESLYPYKNGKTDGVAKSWNKYGKLTYSIEYKNGVENGVYRNWSKNTGKLTK
                     ETQYVNGIRQGVEKEFNDRTGKLLTATQYVNNKRNGTEETYDQNGIKYITCYQNDEEL
                     SSLYTPTQIKDNATKGNSSAQFTLGKYEFTCANIDEGIKWLTKSAEQKNTDAIYFLAT
                     AYKGNGIPANNEKYITYLQQAAMLGNSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAA
                     AQGNFVAHFYLGRMYQNGDGVEKNMEKARFHLSNAAEGGIKPALKALNELEHQTK"
     gene            1399..>2218
                     /locus_tag="ACHKUI_005150"
     CDS             1399..>2218
                     /locus_tag="ACHKUI_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6601140.1"
                     /translation="MKVKSTLLLIILSVFYSHQVLSSAPTQPDVYSVVEQKLNSALPL
                     KENTTFRSQAEWFNLQYKLLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEGA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPDKAMQLLSLDDNALHYYLPTVLV
                     AYQSDPDKAISIYNNVYGDNVIIPYQQATMLLTVAEGYQTKGDIKNAIHYADNALNMF
                     GSRESHYGDEYLKLMNIYATNGNKEKAVVLSQRLQKAITESQSNYLSTLEGILLF"
BASE COUNT          818 a          389 c          431 g          580 t
ORIGIN      
        1 aacgttattt taataacgga aaaattaaag atggtgaatg ccctcatttt tatgaagatg
       61 gaaaaatcaa acagcaacat agctatctga atagtaaact cgatggtccg gcatatgaat
      121 attatcctga tggcaaactc atgcaggaaa atttctatca gcaaagtgag ttgattggaa
      181 aagataccag ttactaccaa tcaggaaaaa taaactctat acacaataga aattcccgtg
      241 gtcagtatga tggaattaac gaacgttata gtgaggaggg taagttactg tctaaatccg
      301 tctataaaga cggaaaacaa atttctgttc aaacctggta tgaaaatggg cagaaagaag
      361 aggagaaaca ttttgatgag caaggccaat taaatggttt ggttaagcaa tggtataaaa
      421 atggaaatct tgcaaaatca caaaactata aacatgacat actcgatggt gatagtgaag
      481 agtggtacga aaatggcatc cctgagagtc tgtatcctta taagaatgga aaaaccgacg
      541 gcgttgccaa atcatggaat aagtatggta aattaaccta ttcaatcgaa tacaaaaatg
      601 gcgtagagaa tggggtatat cgtaactgga gcaaaaacac aggtaaacta accaaagaaa
      661 cccagtacgt caatggtatc aggcagggcg tggaaaaaga gtttaacgat cgcacgggta
      721 aactattaac cgccacgcaa tacgttaata ataaacgaaa tggcacggag gaaacttatg
      781 atcaaaatgg aataaaatat attacgtgct atcaaaatga tgaggaatta tcgtctttat
      841 acactccaac acaaataaaa gataatgcaa caaaaggcaa ctccagcgct caatttacat
      901 taggaaaata cgaattcacc tgtgcaaata tagatgaagg gattaaatgg ttaaccaaat
      961 cggcagagca aaaaaacact gatgcgatat actttttagc tacagcctac aaaggcaatg
     1021 gcatacctgc aaataatgaa aaatacataa catatctgca acaagcagca atgctgggca
     1081 atagtaatgc acaggcggaa attggctatt tatatctcat cggaaaagaa ctaccacaga
     1141 atctgcctga tgctggtgtc tggtttaaaa aagcagctgc tcagggtaat tttgttgcac
     1201 atttctacct gggaagaatg tatcaaaatg gtgatggcgt tgagaaaaat atggaaaaag
     1261 ccaggtttca tctatctaat gcagcagaag gtggaataaa acctgcgctt aaagcattaa
     1321 atgaacttga acatcaaaca aaataaagcg ttcagggacc atgtaaggtt cattaaacag
     1381 aaaaggatat ggattgcgat gaaagtgaaa tctactttgt tattaataat tttatctgtg
     1441 ttttattctc atcaggtttt atccagcgcc cccactcagc cggatgttta ttctgtcgtc
     1501 gagcaaaaac ttaactctgc tttacctctg aaagaaaata ctaccttcag atcgcaggct
     1561 gaatggttca atttgcaata taaattatta gtatctggct accctgaacg tgcttacaat
     1621 ttactgagca aacttgaaac agaatcgaaa aacgctaaat attatctaga tttaactgcc
     1681 tccagagtcg ctgaagaagg agcaccagag actgccttaa cttttttatc aaaaattggg
     1741 ttaaataaac cggcaactct tacatccttt gagagctata tcaatagctg gataaaaagc
     1801 aaacaacccg ataaggctat gcaacttctc agtctcgatg acaatgccct gcattattat
     1861 ttacctactg tgttagtcgc atatcagagc gatcctgaca aggcaataag tatttataat
     1921 aatgtatatg gtgacaatgt tattataccc tatcagcaag ccaccatgtt acttacagtt
     1981 gcggagggtt atcaaacaaa aggcgatata aaaaacgcta ttcattatgc cgataatgcg
     2041 ctgaatatgt ttggcagtcg tgaatcccat tatggtgatg agtatttgaa actcatgaat
     2101 atctacgcta ccaatggcaa caaagaaaaa gcggtagttt tatctcaacg tctacaaaaa
     2161 gccatcaccg aatctcaatc gaattactta agtactctgg aaggaatttt actgtttt
//
LOCUS       DAZFRE010000247         2210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFRE010000247 DAZFRE010000000
VERSION     DAZFRE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.253"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(1..474)
                     /locus_tag="ACHKUI_005151"
     CDS             complement(1..474)
                     /locus_tag="ACHKUI_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601141.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKSE"
     gene            complement(467..880)
                     /locus_tag="ACHKUI_005152"
     CDS             complement(467..880)
                     /locus_tag="ACHKUI_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601142.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(873..1094)
                     /gene="traR"
                     /locus_tag="ACHKUI_005153"
     CDS             complement(873..1094)
                     /gene="traR"
                     /locus_tag="ACHKUI_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO6601143.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(1229..1744)
                     /gene="traV"
                     /locus_tag="ACHKUI_005154"
     CDS             complement(1229..1744)
                     /gene="traV"
                     /locus_tag="ACHKUI_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO6601144.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(1741..2061)
                     /locus_tag="ACHKUI_005155"
     CDS             complement(1741..2061)
                     /locus_tag="ACHKUI_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO6601145.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(2048..>2210)
                     /locus_tag="ACHKUI_005156"
     CDS             complement(2048..>2210)
                     /locus_tag="ACHKUI_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO6601146.1"
                     /translation="PGKMKCIRVVTADEYLKDVYTEDASLPGKIRKCHVYFPDDRTNR
                     SNGDKNEHA"
BASE COUNT          565 a          571 c          474 g          600 t
ORIGIN      
        1 tcattcagat ttactctctt cagcaaactt ccggatggta tcctgttcac gctgacgggt
       61 ggcagactcc tgcattgcat ccactttctc ctgccagaga cgccggatat ccttttcatc
      121 ccgaccagtc agttttttac cggacagcag acggttaagc agttcctgtc tcgcatccgg
      181 tacgtcttca gtcagacgca taagctgaag caggctctga cggctgagaa ccatgagtga
      241 ttccggcagg agggtgaaga agaaaataaa aaagcatacc gcaccacagc aggcaaggac
      301 cgcaaacagg attttcagat atataccatc aacgccgaac gcagcaataa tacagggaat
      361 ccatgcaata cacgcacaat acgccaggat gttatctatt cggttatgtc tgcgatatat
      421 ctgttgatgt tcatccagaa tagaatctat tttttcagct tctgatttat gcatagtgtt
      481 tcctctgcgt ttcctgccag agacgccgga tatcctgttc atcccgacca gtcagttttt
      541 taccggacaa caaacggtta agcagttcct gtctcacatc tggtacgtct tcagtcagac
      601 gcataagccg gagtaattcc tgacgactga gtaccatgcg tgattcctcc ggtacgctgt
      661 aataattaaa aatacgactg aataccccac aacagcaggc aacggacagg cacagggatt
      721 tcagatacag gccagaaaca cagaatgtgg caacaccaca aagaatagct ataactaaaa
      781 atcccttacc cagtgccata cacaaagaac cctgttttct ggatatccgc tcatgctcat
      841 ccagaataaa atctattttt tcattttctg atttatgcat agtgtttcct ctgcctttcc
      901 tgatacgcct gacattcaac gcacaacgtc acaccgggaa atattttccg ccgggcttcc
      961 ggaataggat ttccgcatgc ttcacagaga taaacaggaa taccatgaac atttatttcc
     1021 tggcgtatcc ggtttattcc tgtcatggtc agttgttctg tcactgaata tgcttcatcg
     1081 gcttcatcac tcacggaata ttcctcccga catgttctga cacgctaccg cccatccctt
     1141 ttcacgcaca gggcactcgt tttacaggcg gattttgaat acagaaagcc cgccggcatt
     1201 gccggctgat atacaacttc agggccagtt aattaatacg tggttttccc cacgcagacg
     1261 gtttaataac aaaaaacacg ctggatggct gatgataaac atcctggtta tcaatataag
     1321 gcgctatcca taatgctgcc gtctgctcac ccgttcttag cggacgagga ggtgttaccg
     1381 gcgtaactga actgaccgga acaaccgttt tcacttcccg ggcagcggta aacagcgggc
     1441 gtggtgccag cagtttctgc tccggatgtg ctgtcaccgc aggtctgctg ccggacggag
     1501 tggtcgcggt gacggtctgc accggcattg tccggaagtt cccttcagcc aggcgcggca
     1561 gtgatgccgc aaccggcttt gcttctgagg actgctccag ttttttggcc ttctcattgg
     1621 cctgctccat cgtcatacag gtatcggatg tggtggcgtt acactcaaat tccgtatttg
     1681 ttcctgcgca gccggaaagt aataaggttc ccagcagagg aataaataaa gaaatctgtt
     1741 tcattgccct tctccttttt tattactccc cacaacagca gaaattaact ctgctgtcag
     1801 atttgttttc cctgcacagg gatgtccgga gaccactgta tttcccggag aagaaaataa
     1861 gctcatcgac attcccttct tcatcacacc gacagcagcc atattaaatt tgtgcggcaa
     1921 tgattcagaa ctgaacacct tcggagcagg aagaactatt tttgtggtgc agttactgag
     1981 aacagcatgc ataaaatcat ctgatacgtt tttatccggc acaacgcggg ctgtaataac
     2041 attaatatta cgcatgttca tttttatctc cgttgcttct gtttgttcgg tcatcaggga
     2101 aataaacatg gcatttcctg atttttcccg gcagggaagc gtcttccgta taaacgtcct
     2161 tcagatattc atctgcggtt accacccgga tacatttcat ttttccgggc
//
LOCUS       DAZFRE010000248         2136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFRE010000248 DAZFRE010000000
VERSION     DAZFRE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.254"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            2..448
                     /locus_tag="ACHKUI_005157"
     CDS             2..448
                     /locus_tag="ACHKUI_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO6601147.1"
                     /translation="MYNLWTLRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKH
                     QDEIKSQNPLPEDSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNY
                     LMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
     gene            complement(484..1434)
                     /gene="corA"
                     /locus_tag="ACHKUI_005158"
     CDS             complement(484..1434)
                     /gene="corA"
                     /locus_tag="ACHKUI_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6601148.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1586..1642)
                     /gene="ysgD"
                     /locus_tag="ACHKUI_005159"
     CDS             complement(1586..1642)
                     /gene="ysgD"
                     /locus_tag="ACHKUI_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6601149.1"
                     /translation="MDTPSRYWLIILSSRINS"
     gene            1804..>2136
                     /locus_tag="ACHKUI_005160"
     CDS             1804..>2136
                     /locus_tag="ACHKUI_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601150.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQIERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
BASE COUNT          586 a          512 c          506 g          532 t
ORIGIN      
        1 catctataat ttatggacat tgcgtcctgt acagattttg tatacatact ctgatgctgg
       61 caattcagtc tttctggtcg ttgatcatct gccatggact gatagcgata aaataaattg
      121 gtatctgaag catcaggatg aaattaaaag tcaaaatccc ctaccagaag atagttggca
      181 tacctggtat gttattgata tcggtaatgg cttcactgac tataaaaaat atatcgaagg
      241 gccgtatgaa gatctctact gtttcccgac gattaagagc aatgataatt gcatcgtaaa
      301 aaactattta atggttatta atgaatatcc ttaccgtaat actcattttt ttattgctga
      361 taacaatgag tatcaactaa cccaggaaaa taagatagag cgagtgttca acccgcatga
      421 ctttgaaaaa gacaattttc agcaataagc cggataaaga acaatcttta cccggcattt
      481 atattacaac cagttcttcc gcttaaagta cagatacggt gccaggcccg cgaggatcat
      541 aaagataatc gcgcctgggt agccgaagct ccacttcagt tccggcataa actcaaagtt
      601 catcccgtag ctggaagcga ccagcgtcgg tggcaggaac accacggata ccaccgagaa
      661 gattttgatg atgcggttct gctcgatatt gataaagccc atcgccgcct gcatcaggaa
      721 gttcaccttc tggaacaggg attcgttatg cggcagcagg gattcgatat ctcgcaggat
      781 ttcacgcgcc tgctccagtt gcccacccgg taagcgtgct ttacgtacca ggaagttgag
      841 cgcgcgctgg gtatccatca gacacaggcg aactttccag ccgatatctt ccagttccgc
      901 cagcgtggag agcgcctcgt cgtactcatc gccctgatgc ccttccataa ttacccggct
      961 caactgctcc aggtcgctgt agatattttc aatttcatct gccaactgtt cgattttggt
     1021 ttcgaacaga tccagcagca gctcgtaggc gttaccgtcc accattgact ggctacgggc
     1081 acgcatacga tacagacgaa aagcgggcag ttcacgctca cgcagagtaa acagacgacc
     1141 atcacggatg gtaaatgcca cagtggagtt accggcgtga tcttccgcat cttcaaagaa
     1201 gaagaaggag tgaatatgca ggccgtcatc gtcttcaaag aaacgtgccg atgcttcgat
     1261 gtcttccagt tccgggcggg ttgccaggct ctggccaagt tcagattgta cgcgcagtcg
     1321 ctcgtcgtcg tccggttcga caagatcaat ccatactgca tttacaaggg gttgtgactc
     1381 ttcgacttcc agccgggtca gtcggttatt ttccagttga aatgcgctca gcatgaccgg
     1441 gactcccaat gcttaaaata tcggacagtt cggtgggcac acagaaacaa attgggtttc
     1501 agaccattaa acagcctgac tcagcgcgac gggaaaaatt gaggtcgctg acaaccgcta
     1561 aggctatcag caaaaaggga tagccttagg agttgatcct ggatgacagg ataatgagcc
     1621 agtatctact gggtgtgtcc aaggcgaatg tcctcttaga gtgatcgtgc gcgcatgtta
     1681 cgccagcaaa attttgccgt caacacgcaa cagaacacca cagaacaata gttgaacttc
     1741 gcgctttttg gtactcgtca aggtcgctaa caggataaaa aaatcccaca ttcagggcct
     1801 ataatggcgc atcggctatt cattggtaaa ggaatgatca ccttgaatct caaacggatt
     1861 tttctcaccc tcaccttact tcccttgttt gctgttgccg ctgatgattg cgcaccctca
     1921 gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc aaatagagcg ggtgaaaatg
     1981 tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac atgctctgct ggcggatatt
     2041 aatagtcagg gtcaggtgca gatggcgatg aacggcggca tctatgatga aagctatgcg
     2101 ccgctcggtt tgtacatcga aaacggtcag cagaag
//
LOCUS       DAZFRE010000249         2118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFRE010000249 DAZFRE010000000
VERSION     DAZFRE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.255"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..561
                     /locus_tag="ACHKUI_005161"
     CDS             <1..561
                     /locus_tag="ACHKUI_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6601151.1"
                     /translation="SSDNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPV
                     NWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGV
                     QAGYAHSVSGNSAEGYNGQATLNVTF"
     gene            656..1783
                     /locus_tag="ACHKUI_005162"
                     /pseudo
     CDS             656..1783
                     /locus_tag="ACHKUI_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1859..>2118
                     /locus_tag="ACHKUI_005163"
     CDS             1859..>2118
                     /locus_tag="ACHKUI_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6601152.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEA"
BASE COUNT          493 a          566 c          594 g          465 t
ORIGIN      
        1 tcatcggaca ataacgactt ccgcgcccgg ggccggggct ggcagggctc actggaaacc
       61 ggtctgccat tcagtatcac tgacaacctg atgctggagc cacaactgca gtatacctgg
      121 cagggacttt ccctggatga cggccaggat aacgccggtt atgtgaagtt cgggcatggc
      181 agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggt
      241 gaaggcacct catcccgtga caccctgcgc gacagtacaa aacacggtgt gagtgaactg
      301 cctgtgaact ggtgggtaca gccttctgtt atccgcacct tcagctcccg gggagacatg
      361 agcatgggta cagccgcagc cggcagtaac atgacgttct caccgtcccg gaatggcacg
      421 tcactggacc tgcaggccgg actggaagcc cgtgtccggg aaaatatcac cctgggcgtt
      481 caggccggtt atgcccacag tgtcagcggc aacagcgctg aaggctataa cggtcaggcc
      541 acgctgaatg tgaccttctg acagaaccat cgcctctttg tggtcccggt catcatgatc
      601 gggacccgga ccggcgcaac ggatattcaa cgccacattc gctggcatta acagtgcggc
      661 attcgacgga cagaacggac agtatcgtta catgggaaat catggtccca tgcaactgga
      721 agtcccccgt gatcagtatg ccggcgctgt ggaaaccatg aagaataaga tccgcgaagg
      781 taaagtaccc ggtgtaaccg atcccgcaga agcgtcccgg ctgattcgtc ggggacatct
      841 gacttatacc caggcccgta atatcacccg gttcgggacc atcgaatcgg tcacttatga
      901 tattgccgag gggtcggttg tcagtctggc ggccggaggg atcagttttg ccctgacggc
      961 atcggtcttc tggctcagca ccggcgatcg cgatgctgcc ctgcagacag ctgctgtcca
     1021 ggcaggaaaa accttcaccc gcacactggc tgtctacgtc acaacccagc aacttcaccg
     1081 gctcagtgtt gttcagggta tgctgaagca tattgatttt tcgacggcca gcccgactgt
     1141 ccggcaggcg cttcagaagg ggaccggtgc aggaaatatc agtgccctga acaaagtgat
     1201 gaagggttcg ctggtgacat ctctggcact ggtagctgtc acaaccggcc ctgacatgat
     1261 caaaatgttg cggggacgga tctccggtgc gcagttcatc aggaatcttg ccgtggcatc
     1321 ttcctgtgtg gcaggtggtg ctgtcgggtc agtggcgggc gggatattgt tcagtccact
     1381 gggaccattt ggtgcactga cagggcgtgt ggttggcggt gttctggggg gaatgattgc
     1441 ctccgctgta tcaggaaaaa ttgccggagc gctggttgaa gaagatcgcg tcaaaattct
     1501 ggcaatgatt caggagcaga tgacatggct tgccggcagt ttcctgctga ccggacatga
     1561 gattgaaaat ctgaacgcga atctggcccg tgttatcgat cagaatgctc tggagatcat
     1621 tttcgccgcc ggtatacaac aacgggccgc gaccaacatg ttaatcaaac cactggtggt
     1681 cagtatcatc aggcaacgcc ccgtcatgga atatgatgca tcccatctcg gcaatatggt
     1741 taaccgactg gaagaaacat tacccccgga gttaccggca taacggctat tctgcgcctt
     1801 ttacgtttta tcacctgatt tgtcagtgtc atcgtcatga ctcagagagg taaataccat
     1861 gattcacctg ttcaaaacct gcatgattac cgccttcatt ctggggttaa cgtggtctgc
     1921 cccactccgg gcacaggatc aacgttacat cagtatacgc aatacagata cgatatggct
     1981 cccgggaaat atttgtgctt accagttccg gctggataat ggcggaaacg atgaaggatt
     2041 tggccccctc accatcactc tgcaactcaa agacaaatat ggtcagacgc tggtgaccag
     2101 aaaaatggaa acggaagc
//
LOCUS       DAZFRE010000250         2113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFRE010000250 DAZFRE010000000
VERSION     DAZFRE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.256"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..397)
                     /locus_tag="ACHKUI_005164"
     CDS             complement(<1..397)
                     /locus_tag="ACHKUI_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601153.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSSVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVT"
     gene            complement(479..1507)
                     /locus_tag="ACHKUI_005165"
     CDS             complement(479..1507)
                     /locus_tag="ACHKUI_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFO6601154.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFSHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQVSCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFGQ"
     gene            complement(1533..>2113)
                     /gene="rfaY"
                     /locus_tag="ACHKUI_005166"
     CDS             complement(1533..>2113)
                     /gene="rfaY"
                     /locus_tag="ACHKUI_005166"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO6601155.1"
                     /translation="LRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYY
                     QNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDV
                     KAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERH
                     LGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          655 a          431 c          345 g          682 t
ORIGIN      
        1 aagtaacacc attttttata tgatggccat aaagcccaca ataaatcgca tcccctcccg
       61 tctcatgttg tctggaatac atcttcagca gatagttagg atctatctca tcatcatcat
      121 ccataaaaca tataaactgt ccttgcgcct tgcggatacc tgtattcctg gcagacgaaa
      181 caccgccatt ttctttattg ataataatta atcgagagtg ggttaaccca tattctctca
      241 aaacacttga tgtgttatct ttagaaccat cattaacaat aacaatttca aaatttgggt
      301 aagtttgttt ttctaatttc gttagagttt cagtaatgta atcagatgaa ttatacgtcg
      361 gtattattat ggatactaaa gggtaatcat tgctcatcgt gttctcttgc ctgaactaat
      421 aaaatattat agtactcatc cttaattatt attgtaactc agacatccat gatttttatt
      481 attgcccaaa tagttttctt ttatacttat ttaatttgaa tttaatgaag taaataaaag
      541 cgttaaacgt atcaccattt ttctttgcat gttggtattt cacttgatac tgcttttcat
      601 tcgtggctgg aataaacgcc acatcattcc agcaagaaac ctgataagcc tcattaaaat
      661 acttctggca gggataattc gtccagtcgt tccagggctt cgttacgcca acataatgaa
      721 tcagcttagt atcatcggta atggtctttt tataatcttc atcgctttta ttttttaact
      781 catagtcaat gccataaaag caatcataat cgccgctaat aaaaatacaa tgactaaaga
      841 acagaatatt taaaatatcc tgatcaaggc atttatattt cgtgtgctgc gggtcacttg
      901 ccagcatagc aatggcttta ttcataaaat catttttcgc ccatgcttca agctgcagat
      961 atactacacc ggcgttgaaa taattccctg gaagtccctc tatagccaga cgcttcgctg
     1021 gtttttcctg cataaaaggc acatctttaa taacagccgc gaacttatcg ccatcaaaat
     1081 taatatcaag caatccagct aatgagcctt tacagatgac atcagcatcg atatataaca
     1141 gattttcaat agtttcactc agatactgga acgcaactaa acggaagaat gttgcataag
     1201 accactgttt caggctgggg aagatatcaa taaaggcact ggatacttta taaattctta
     1261 cttcaacgtt agcatttttg ctggtaaaag cattaacacg ttgtagataa tcttcatcaa
     1321 tgtagtcagt aaagagatga aacttaaccg caaggtccgg gttatgcata acaactgatt
     1381 gcatagatac tgccgcacca aataaaaagt tgcggtcaat accgtaagcg atatttaaca
     1441 cacgatcggt gttagcaatt tccccagaga cagcgacttc agtaatactc tcagctaata
     1501 aatccatgtt ggttccgttt tgactgtgtg gtttacgctt tgccttttaa ttttttaata
     1561 aatttgcgta gtttggttct gtagattact gaatagtagc cataatcttt tatttcgttg
     1621 gcaataccca ggtgacgttc catggccaga cgatcacggg ctttccgctc tgccgtacag
     1681 cttttaccgg ataggtcaat tatcctgaca ccatctttac taacgataaa gttaccccgg
     1741 tgtgggtcgc ccgatagcat atttaaggca tgcaattttt ccattgaagc cttaatttct
     1801 gctttaacat cttctggaat aatcggcata tcattgagct ctacgccctc gacatactcg
     1861 ataagcatga taaaaacact ggcatagttg aaaattttac gctcagccag aaaataaaaa
     1921 tcattaggaa aagtaagacc tgcacttctg acacgatcgg tttcaacgat caaattctga
     1981 tagtagtcac cttttacaaa agattttaaa aaacgttcat tccttttcgt tttcggggca
     2041 aagactttaa aaacatagcg accatatttc gtatcaatca acgatacttt ggtatcatca
     2101 atattacgta ata
//
LOCUS       DAZFRE010000251         2104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFRE010000251 DAZFRE010000000
VERSION     DAZFRE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.257"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1506
                     /locus_tag="ACHKUI_005167"
     CDS             <1..1506
                     /locus_tag="ACHKUI_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6601156.1"
                     /translation="TRFTLTEHPQKMLNREWQVVQSILSGDQPQALHGSQGRGTTLGN
                     QLEVIPADRTWRPRVQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVKFHWDRYHGMT
                     EESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPVVMGRTYHEDNRSPGS
                     LPGTKTQMTIRSKTYKGSGFNELRFEDATGGEQVYIHAQKNMDTEVLNNRTTDVKADH
                     TETIGNDQKITVVKGQTVQVGTRKEGGHDQSITVANDRCITVRNDQTLQVTNDRTVSV
                     SNDDGLYVRNDRKVTVEGKQEHKTTGNHVSLVEGKHSLVVKGDLARKVSGALGIKVDG
                     DIVLESSSRISLKVGGSFVVIHSGGVDIVGPKISLNSGGSPGTPVPALQPAVLKTLGD
                     EKSGDGSDSGEENEDSGGNCVTGSGGDDRGDDEDEPEKYTLQFHFTDDDGIPYSETRY
                     IAFFEDGTQTRGETDEEGYTERFFVSSKHEIKVKLLFANDDFLSMEGHYGR"
     gene            1496..>2104
                     /locus_tag="ACHKUI_005168"
     CDS             1496..>2104
                     /locus_tag="ACHKUI_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M35"
                     /protein_id="HFO6601157.1"
                     /translation="MDDNNQTSGQPKPEPEECVKEQKITDHFKIMIDKARKAQKLVLI
                     KRADDLLRWGAQEEYDFSKIFGVKGNKEVNIRKYGHNTGRRMNARFLMMDGVRRLMII
                     ANDLTMSSFINYTGCNEFAAFVSPSKDMPYIINIGAKFEYRDGKKNPVTGKDSHVATL
                     CHEMSHIQWYYGDNKKGGMWSQDYTTTDKYSTCKEDEVSYDEH"
BASE COUNT          617 a          376 c          682 g          429 t
ORIGIN      
        1 acgcggttta cgctgacgga gcatccgcag aagatgctga atcgtgaatg gcaggtggta
       61 cagagcattc tgtcaggaga ccagccgcag gcgcttcatg gcagccaggg gagaggaacc
      121 acgctgggaa atcagttaga ggtgataccg gcggaccgga cctggcgtcc acgggtgcag
      181 agcaaaccga aggtggacgg gccgcagagt gcgatagtga cggggccagc gggagaggag
      241 attttctgcg acgaacatgg tcgggtacgg gtgaagtttc actgggaccg ttatcacggg
      301 atgacggagg agagttcgtg ctgggtgagg gtgtcacagg cgtgggcggg gccggggttc
      361 ggtaacctgg cgataccgcg tgtgggtcag gaagtgatag tggacttcct gaacggggac
      421 ccggaccagc cggtggtcat gggcaggacg taccatgagg acaaccgttc gccggggagc
      481 ctgccgggga cgaagacgca gatgacgata cggtcgaaga cctataaagg aagcggcttt
      541 aatgaactgc ggtttgaaga cgccacgggc ggtgaacagg tctatatcca cgcgcagaag
      601 aacatggata cggaggtgct gaataatcgg acgacggatg tgaaggctga ccacacggag
      661 accattggta acgaccagaa gataacggtg gtgaaggggc agacggttca ggtcgggacc
      721 agaaaagaag ggggccacga ccagagtatc acggtggcga acgaccggtg catcacggta
      781 cgtaatgacc agacgctgca ggtgacgaat gaccggacgg tgagcgtgag taatgatgac
      841 ggcctgtacg tcagaaatga ccgtaaagtg acggtggaag ggaagcagga gcataagacg
      901 acggggaacc atgtcagcct ggtggaggga aagcacagcc tggtggtgaa gggggacctg
      961 gcgaggaagg tgagtggcgc gctggggata aaggtcgacg gggatatcgt gctggagagc
     1021 agcagccgga taagcctgaa ggtgggtggc tcgtttgtgg tcatccattc agggggcgtg
     1081 gacatcgtcg ggccgaaaat cagtctcaat agcggcggta gtccggggac gccggtgccg
     1141 gcgctgcaac cggcggtgct gaagacactg ggggatgaga agagtggtga tggcagtgat
     1201 tccggagaag aaaatgaaga ctctggaggg aactgcgtga ccggaagtgg tggtgatgat
     1261 cgaggtgacg atgaggatga acctgaaaaa tatactctcc agttccattt tacagacgat
     1321 gacgggatac cgtattcaga aacccgctac attgccttct ttgaggatgg cacgcagacc
     1381 cgcggagaaa cggatgaaga ggggtataca gagcgttttt ttgtatctag caaacatgaa
     1441 attaaagtga agttgctatt tgcgaatgat gattttctct caatggaagg acactatgga
     1501 cgataataat caaacttctg gacaaccaaa gccagagcca gaagaatgtg tgaaagagca
     1561 aaaaataaca gatcatttta aaataatgat tgataaggct cgtaaggcac aaaagttggt
     1621 tttgataaaa agagcagatg atcttttgag atggggagca caggaggaat atgatttcag
     1681 taagatcttt ggtgttaaag gaaataagga ggttaatata agaaaatatg gacataatac
     1741 aggtaggcgc atgaatgcta gatttcttat gatggatggt gtgagaagat taatgattat
     1801 agctaatgat ttaacaatga gcagttttat aaattataca ggttgtaatg aatttgcagc
     1861 attcgtcagc ccaagtaagg atatgcccta tataattaac ataggcgcaa aatttgagta
     1921 tagagatggc aaaaaaaatc cagtaacggg aaaggattct catgttgcta ctttatgtca
     1981 tgaaatgtct catattcagt ggtattatgg ggataataaa aaaggtggta tgtggtcaca
     2041 ggattataca actactgata aatattcaac gtgcaaagag gatgaagttt catatgacga
     2101 acat
//
LOCUS       DAZFRE010000252         2102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFRE010000252 DAZFRE010000000
VERSION     DAZFRE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.258"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..330
                     /locus_tag="ACHKUI_005169"
     CDS             <1..330
                     /locus_tag="ACHKUI_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6601158.1"
                     /translation="DPRKPISENTVNKALRVMGYDTTKEVCGHGFRAMACSALIESGL
                     WSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREECISPFEYAK
                     VNNPLKR"
     gene            586..1629
                     /locus_tag="ACHKUI_005170"
     CDS             586..1629
                     /locus_tag="ACHKUI_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6601159.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWILPF
                     SAAGMKHTGIQYQ"
     gene            1772..2002
                     /locus_tag="ACHKUI_005171"
     CDS             1772..2002
                     /locus_tag="ACHKUI_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601160.1"
                     /translation="MNYPMDSLTPFHDALLSNTLWLTSKKTGFPLPAGNHLPGVFGWS
                     GYSCLVISKIIAINSGKLIVHNLVMNITLIFP"
BASE COUNT          594 a          461 c          473 g          574 t
ORIGIN      
        1 gacccacgta aaccaatcag tgaaaatacc gtaaataagg ccctcagagt gatggggtat
       61 gacacaacca aggaagtctg tggccatgga ttccgggcga tggcgtgcag tgcattgatt
      121 gaatccggct tatggtcaag agatgcggtt gaacgtcaga tgagtcatca ggagcgcaac
      181 ggagtacgtg ccgcgtatat tcataaagca gaacatctgg aagaacgtcg gcttatgctg
      241 caatggtggg cagattttct ggatgcaaac agggaggagt gtatcagtcc gtttgaatat
      301 gcaaaagtta acaatccact gaaaaggtga ctgaacgcca tttacacaca tgctcttcct
      361 cttaagggga agagcacccg cctcaatcaa tttctttttt atgccggcgg aaccatcagc
      421 caggctgagg ttctcattca ataactcaac gtcttaccac agaacagaat tcactgtatt
      481 atcatgggca acgttaatcc cggacaataa aaggacaccg atgccaggca tcacaatttt
      541 tatagcctgc aggttatggt ccatcctttt ttacaggttc agaatatgac tggcaaatta
      601 cgctttgaag tgaacgataa ccagggctgt ttcatatttc cggaaacctg gtttggctcg
      661 ttgctggatg agtttgaaga actgattgat gcatacgatg cggatgaaat cagcgaaaca
      721 agttacatca acaaactcag gcgccttgcc cgacaggaga atgactttat tgatgtacac
      781 gctcacttgg catacgtatt tctggagcag aatgccccgc gtaaggcgct gaatgctgcc
      841 cttaaagggc tggcaattgg caatcgtctt atccctgaag gtttcagtgg cagaatcatc
      901 tggatacatc ctgacaaccg accttttctt cgtgccctct atgcggcaat cctggcaaat
      961 gcacatttac agcgtcatca ggatgccatc atgctcattg aaaaaatact ggattacaat
     1021 cccgaagata atcatggtgc acgctggcta ctgggaccgg aactgttacg cactggcgct
     1081 catgaacagg cacgccatat actgcaggaa catgcagatg aattttcacc ttactggtat
     1141 gaactcggcc ttctgcattt tcttaatggg gaactggtaa aagctgcaac tgcatttcgt
     1201 cgcggctttg cggcaaatac ttacatcgca gaaatactgt gcggaaatct gcatccattt
     1261 ccacttgctg tatggcataa cttttcaggc ggtccggata ctgccgaaga ttattatgcc
     1321 acataccacc cgttatgggg gcaatatccg gaagcattgc tgtttgtaaa ctggctctat
     1381 aatcattcct ctgtgctgca tgaacgggca gaaatcatta aatgcgccga aatgctgatg
     1441 caggaagatg atttcgaaat atgcgaaagt attttaagac agcaggagaa gttgcgtgaa
     1501 agaattgatg agacgctttc tgagaaaatt gtacagaaat gcagaaatat gaatggtgaa
     1561 tatatctggc cctggatatt gccgttttca gcggcaggca tgaaacatac tggcatacag
     1621 tatcagtaga tattgcatta gtgtatcctg cacacaagta ataatttatt caccaataat
     1681 aacactgtta atgccccctt cccctggttg tcagccaggg gttatcttct gaatatttct
     1741 tttgaaaagg ataacacagt aaattatttt tatgaattat cccatggact cattaacacc
     1801 ctttcatgat gctttattgt caaacacgtt atggctgaca tcaaaaaaaa ccggatttcc
     1861 tctgccagcg ggtaatcacc ttcccggtgt tttcggttgg tctggttact cctgtctggt
     1921 tattagcaag ataattgcta taaacagtgg aaaactcatc gtacataatc tggtgatgaa
     1981 cattacgctt attttccctt gaccggaaga atcagaggct gtggtttcag actgtctgcc
     2041 agtacattcc tctctccgtt aaaaaccata acgggttcat tatcttcgtc tgtcagcaga
     2101 tt
//
LOCUS       DAZFRE010000253         1987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFRE010000253 DAZFRE010000000
VERSION     DAZFRE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.259"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            17..714
                     /locus_tag="ACHKUI_005172"
     CDS             join(17..266,266..714)
                     /locus_tag="ACHKUI_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFO6601161.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMGEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            986..1537
                     /locus_tag="ACHKUI_005173"
     CDS             986..1537
                     /locus_tag="ACHKUI_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6601162.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMILTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          584 a          425 c          468 g          510 t
ORIGIN      
        1 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
       61 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
      121 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
      181 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
      241 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
      301 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg gcgaacagtg gggctatgtc
      361 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acaggctccg gaagacggtt
      421 gttgcgcacg tattcggtga acgcacgatg gcgacgctgg ggcgtcttat gagcctgctg
      481 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
      541 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
      601 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
      661 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
      721 gtcattacct acaacgttta tcgacctcac acgaacaacg atggtttacc gccttgctta
      781 catgaagaaa agtggaaaca ggttattccg gtgtcctgat tttgtgaacc actacaataa
      841 tcctttttca cagacttgtc agcagccagc atttatgttc ttttccctgc gggaatttat
      901 ccgtctgcct gtcgggacta ctctgtcata cagaaatcag gtaggaataa attgtagtgg
      961 aaagtcgatg tttaccggat gactgatgcg cgcttgtaca cagacagtgt gtttcagtaa
     1021 tatggagaat aatgaaatga ataacacaga cacattagaa aaaataatca gacaccaaaa
     1081 aaacaaagac cccgcatatc cctttcggga acatttgttg atgcagctct gtattcgcac
     1141 aaataaaaga atgcaggata atatatctga atttctgggg gtgtatggaa taaatcactc
     1201 agcatatatg attctcacca cattattcgc agcggagaac cattgtctgt caccttcaga
     1261 gataagccag aaacttcagt ttaccagaac taatattacc cgcattacag attttttaga
     1321 aaaagccgga tatgtaaaaa ggacggatag cagggaggat cgccgtgcta aaaaaatcag
     1381 tctgacatct gaaggtatgt tttttattca gaggctcact cttgcacaaa gcatgtatct
     1441 gaaagaaatc tgggattatc tgacccatga tgaacaggaa ctgtttgaag tcattaataa
     1501 aaagttactg gcacattttt ctgatgccag ctcataaagt gcgaaatatc tgaggatgct
     1561 ggatagcttc aggcaagaga ataatgattc ttgcagatgt gtttttccgg atacaaaaac
     1621 aaaagtaaaa aattgcagcg ccaggccacc tttcaaagca gggaggcctg taccgcgtcg
     1681 aaaatttcag ccagttaatg taattgtctg aaccaggcac ttcgcccggg caggagaagg
     1741 agcgtggttg cggtcatcct gtatactgaa gtcgttatga aggaataata caaataattg
     1801 cccgttttct ctgtacgata gaggcaggtg tgtatgattg cattactatg tttttgtgat
     1861 ataaaaaact aaatggtgag atatgcattt taaaatataa caaaaatcac catgaaataa
     1921 gaataattat ccacggagaa cataaactgc agactgttac aatgcatgct gttcatcttg
     1981 acaatgt
//
LOCUS       DAZFRE010000254         1985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFRE010000254 DAZFRE010000000
VERSION     DAZFRE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.260"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..667
                     /locus_tag="ACHKUI_005174"
                     /pseudo
     CDS             1..667
                     /locus_tag="ACHKUI_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(636..824)
                     /locus_tag="ACHKUI_005175"
     CDS             complement(636..824)
                     /locus_tag="ACHKUI_005175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601163.1"
                     /translation="MKINLLRGYLLSATQHDNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            846..995
                     /gene="mok"
                     /locus_tag="ACHKUI_005176"
                     /pseudo
     CDS             846..995
                     /gene="mok"
                     /locus_tag="ACHKUI_005176"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            937..1062
                     /locus_tag="ACHKUI_005177"
     CDS             937..1062
                     /locus_tag="ACHKUI_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO6601164.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(1516..1689)
                     /locus_tag="ACHKUI_005178"
                     /pseudo
     CDS             complement(1516..1689)
                     /locus_tag="ACHKUI_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          402 a          549 c          598 g          436 t
ORIGIN      
        1 aacacgctgg cggaatatcc gtatgcgggg gcgtttttcc gctgtctgaa cggaagccgc
       61 aggatatcgc tgtctgacct gcgatttttc atgccgtccc tgaccgcaga agaactgcac
      121 ggcaaccgtc ttcagtggct gtatgccatc gatgtgctga ttgaaacaca gggcgaggtc
      181 tgcctccttc cccttccagg cgatgcagca gaacgcttgt tcccgtcggt gcggtttcgt
      241 gtcagggaac gcagtcgcca taaatccgcg ctggtcatgc agaagtacag ccgccagcag
      301 gcgagggagg cagagcagaa agcccgtgcg taccaggcgc tggtcgccca ggctgaaatc
      361 gaactggcgt tccattcacc ggaaacagtc ggaagctggc atgcacgctg gagtgaccgg
      421 gtggcagagc atgacctgga aaccctgttc tggcagtggg gcgagcgttt tccgtcactg
      481 gccggaatgg aacgctggca gtggcaggat atgccgttct ggcaggttat tgcggaagcc
      541 agtcttgcag caagagaggc cggtcacgct gtccgtgaga tggagcggtg gatggtgccg
      601 aacaaactcc gggaggcagc gtgatgcggc aacaatcaca cggatttccc gtgaacggtc
      661 tgaatgagcg gattattttc agggaaagtg agtgtggtca gcgtgcaggt atatgggcta
      721 tgatgtgccc ggcgcttgag gctttctgcc tcatgacgtg aaggtggttt gttgtcgtgt
      781 tgtgtggcag aaagaagata gccccgtagt aagttaattt tcattaacca ccacgaggca
      841 tccctatgtc tagtccacat caggatagcc tcttaccgcg ctttgcgcaa ggagaagaag
      901 gccatgaaac taccacgaag ttcccttgtc tggtgtgtgt tgatcgtgtg tctcacactg
      961 ttgatattca cttatctgac acgaaaatcg ctgtgcgaga ttcgttacag agacggacac
     1021 agggaggtgg cggctttcat ggcttacgaa tccggtaagt agcaacctgg aggcgggcgc
     1081 aggcccgcct tttcagggat gatgctggtc tgactgcact gaagcgcctt tataaagggg
     1141 ctgctggttc gccggtggcc cctttctcct tgctggcatt gtgcgagcat gaacaaccag
     1201 actgccagga aggccgtttc cctccgggaa cggggaagaa cgcgcgcctg cggcgcccgt
     1261 tcttttcccc cgccctctct gtttatggcc agttgtcaga aatgacatta cctttcccgt
     1321 gattattctc tttttcttcc tgctctgatt ctgactactg cagtgttgtt ctgtctgccg
     1381 ggcgcacctt cggctcccgg ctgcctggcg gcatccggtc gtcaggcccg actccgacag
     1441 gcagctgctg gcgtacctgc cagtctccgc cgaacccgct ggcgcggttt cgggctgtcg
     1501 ctacggtccg atacgtgaaa acccccggct tgcagcgacc gttccggtcg cgccgcctct
     1561 gtcgcgccgt ggcctgcgct cctggcatcc cggcaaaagg ccgacgggat gtaaggcgca
     1621 acgccgtttc gcaaaccgcg cttcacgtcg ttgcggcggg tccctgcccg ttgttgtgtc
     1681 cggtgccatt cacctgttta agccctgtcg ccgggggctg atacagccac aacctgccgc
     1741 actgactgtc cagggtaaat gaacagccca caaaattttt tacccaaaac ccggtaaaaa
     1801 attttgcgtt ctgcccagga caggtgcgtc aggccgtggc agtgatgccc catgcggcga
     1861 aggttctcaa acaggtgatg gtgaataccg gagggaacaa agagcaggtc cccgcaaaac
     1921 gtcaaaaccg cggctcccga aactcaacag atgatggggg ctgaaaacca gagagaggag
     1981 aaagc
//
LOCUS       DAZFRE010000255         1937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFRE010000255 DAZFRE010000000
VERSION     DAZFRE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.261"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..657)
                     /locus_tag="ACHKUI_005179"
     CDS             complement(<1..657)
                     /locus_tag="ACHKUI_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6601165.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVAIVFKNSYIIGYYENVQSGIDEYKMY
                     Q"
     gene            complement(654..1430)
                     /locus_tag="ACHKUI_005180"
     CDS             complement(654..1430)
                     /locus_tag="ACHKUI_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6601166.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGIGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
BASE COUNT          585 a          439 c          347 g          566 t
ORIGIN      
        1 ttgatacatt ttatactcat ctataccact ttgaacattc tcataataac caataatata
       61 gctattttta aaaacaatcg ccacatcaca aaactctttc aatgaattca aactatgtga
      121 aaccatcaaa aagctagact ctttatgcct ttctttaaac aattgagcgc acttctcttt
      181 aaacctggca tcaccgacag ctgttacttc atcgacgata taataatcaa acttaaatgc
      241 catgcttaaa ccaaagccta aacgcgatcg catacctgag gagtaagttt tgatcggcat
      301 atcaaagtac ttaccgagtt cggcaaattc ttcgacaaac tcaattttct ctttcagctc
      361 ttcttgcttc gcgtataacc gcgcaacaaa ttttacgttt tcgcgtccgg ttaaacttcc
      421 ctggaatcca ccggcgaggc cgactggcca tgatatcgtt ttattggtga tgatctttcc
      481 gctatcgggg cggtcaatgc caccaatcat tctcagtaac gttgatttac ccgcaccatt
      541 acgaccaata aaggcaacgc tttttcctga agggatttcg atgtttaaat ccttaaacac
      601 atagtgtcgc cccgttggcg tacgatatga cttcgtcaaa ttctcaatct taatcatgat
      661 gtcagcattg cctcctcacg agttcggtat aacgccagac cgatgaacag ggtgaccaga
      721 gtgaacattg caaggtagtt cagactcacg ccttcgctga tatagccagg cataactgcc
      781 tcgcggctta actctacgac atgcactaat gggttccaga gtagatatga ccagtattgc
      841 ttcggaatcg aatgtagagg gaacatgatg caggagatga aatacagcgg cttaagcagt
      901 atcggcagga ccttttgcat ttcagggaag gttttaccaa cgaccatgaa tattaagccg
      961 atgccacatg aaagaatgat taacaaactc caggtgagca ctagttgtaa aaagtttgtt
     1021 atctcgaaat attcacctgc catccagacg ataagcatga gcaatatata aacagcaacg
     1081 taaatcagcg tctcaagcag tgcgcgtgca atgatcgtat cgatgggttt tactggtcga
     1141 taattaaaca acccctggtt cgcttcaata gcgcctacag aacgattgct gatactgcta
     1201 aagataaaaa agggaatcag gccattaagt aaaaacaccg ggaatgagat gtctggcatc
     1261 gtgcggtgca taatgtaacc aaaaatgccc aacagtatca gcaaatgcgc agagggttcg
     1321 agaatcgccc acaaatatcc cagacggaac ttaccaaagc gtgttcgtat ttctcgtaga
     1381 aataatgcct ctacggtgac tttctggact tcaaatccac ttcttgccat ttgatgatgt
     1441 gatcctaatc tcttcaggta cgctaccgcc cttggcttaa cagctaccaa tacactggag
     1501 gtaatgctta tttgtaactg gataaaattc actcagacgg tttgaccttt ttaatagtcg
     1561 caaaaaaaat ttcagcagaa attatttccg cagggataaa tagatctatt tccgtctcgt
     1621 ttcttctaat ccattcttca ggcatgaatg gattcggtct tctggtaatt tcagaaaact
     1681 ggccgtttta gaatgcagat ttaatttctc aggaaattat tttttattat tactgttaac
     1741 tacttgatta taataccccc tatggatttt atgcgtaaca atacctgcaa tccgtttcaa
     1801 accagaacgc acgcgattga ttaccaataa attgttcacc aagaaactat ttccctattt
     1861 aaaattcact cgtgtacttc ttatttatat ctacagcccc ctctttacag ccatatttgt
     1921 gatttatatc acattta
//
LOCUS       DAZFRE010000256         1922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFRE010000256 DAZFRE010000000
VERSION     DAZFRE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.262"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1194
                     /locus_tag="ACHKUI_005181"
     CDS             <1..1194
                     /locus_tag="ACHKUI_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO6601167.1"
                     /translation="VQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDC
                     GETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRA
                     ASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVREN
                     WQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVND
                     GFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNR
                     KLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQS
                     KTWVLFGKTNHGKLSGEILYWLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAM
                     AFASI"
     gene            1278..1616
                     /locus_tag="ACHKUI_005182"
                     /pseudo
     CDS             1278..1616
                     /locus_tag="ACHKUI_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1696..>1922
                     /locus_tag="ACHKUI_005183"
     CDS             1696..>1922
                     /locus_tag="ACHKUI_005183"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6601168.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPIT"
BASE COUNT          501 a          463 c          543 g          415 t
ORIGIN      
        1 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
       61 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      121 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      181 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      241 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      301 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      361 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      421 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      481 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      541 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      601 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      661 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      721 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      781 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      841 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      901 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      961 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1021 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt actggttggt ggggcaaggc
     1081 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1141 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1201 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1261 actacgccct gcctcttact ctactcagca catggcaggg ggtcgtctat gtggcgttca
     1321 tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga
     1381 cattcgtgct ggatgctctg gagcaggcgt tatgggcccg tcgaccgtcc ggcacagtcc
     1441 atcacagtga taaaggttct cagtatgtat cgctggccta cacacagcgg cttaaggaag
     1501 ccggattact ggcatcaaca ggaagtactg gtgactcgta tgacaacgcg atggcggaga
     1561 gcatcaatgg cctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtaagc
     1621 gtcgtctcag caccgtctgg cagatcctga aattcctgag agaatagtgg acaccaaata
     1681 tggtggacgc tatccatgaa atcattaacc gcagtgcgta aaaaaagccc taattatccc
     1741 gttgagttca aaatcaaaat ggttgaactc tcgcatcgac cagagatctc cgtagcgcaa
     1801 ctcgctcgtg agcatgggat caacgataat ttgctgttca agtggcgcca gtactggcgc
     1861 gaaggaaaac tacgtcctcc ttcaacaaca gaaaacaacg tgcctgagct gctcccgata
     1921 ac
//
LOCUS       DAZFRE010000257         1909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFRE010000257 DAZFRE010000000
VERSION     DAZFRE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.263"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1163
                     /gene="istA"
                     /locus_tag="ACHKUI_005184"
     CDS             <1..1163
                     /gene="istA"
                     /locus_tag="ACHKUI_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISEc10 family transposase"
                     /protein_id="HFO6601169.1"
                     /translation="AEQEVMFRQRHQPGLRGLSDFTELKGVVVTIAGKLLAHKLYHFR
                     LEWSHWSWMRVVLGGESFSALAEGLQEALGQLGGVPVEHKTDSLRAAWKQQGEDGRRE
                     LTERYAALCQHYGMQGVHNNAGRGHENGSVESAHGHLKRRICQALILRGSNDFSTIEE
                     YQAFITQQVMRHNRNNQDLVKEERLHLKPLPLRRSADYDELTVRVSRSSTINVKHVVY
                     SVPSRLVGQLLRVRLWDDRLSCYVGSSEVMSCPRVRPEKGKTRARRIDFRHVIDSLAK
                     KPGAFCHATLRNDILPDDEWRRLWRRLCNHLEPDMAGRLMVHALKLAAGYDDISVVAK
                     GMEQMLNTPGNVDLHRLMRFLGIKEKALPVVNVKQHNLSSYEQLLRGKGGSQ"
     gene            1160..>1909
                     /gene="istB"
                     /locus_tag="ACHKUI_005185"
     CDS             1160..>1909
                     /gene="istB"
                     /locus_tag="ACHKUI_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
                     /protein_id="HFO6601170.1"
                     /translation="MSNIHHLERSLRKLRLTRVGAEWHALEKRALAEGWTPSRYLLTL
                     CNEELLWRESEKLRRYKKEARLPVAKTLSEYDFSQVPELNGAQFRQLCETTDWVDAGE
                     NVLLFGASGLGKSHLAAAIVDGVVGQGYRARFYSAGELLQELRKARAQLKLNELLLKL
                     DRYRVIVVDDLGYVKRDSAETGVLFELIAHRYERGSLVITSNHPFSMWGSIFVDETMA
                     VAAADRLIHHGYMFELKGESYRKKTAKAVTSA"
BASE COUNT          485 a          447 c          592 g          385 t
ORIGIN      
        1 gtgcagagca ggaggtcatg ttccgccagc gacatcagcc cggtctgcga ggtctgtcgg
       61 actttactga actgaaaggt gtagttgtca ccatcgccgg taagttgttg gcgcataagt
      121 tgtatcactt ccgtctggaa tggagccact ggagctggat gcgggttgtg ctgggtggtg
      181 agagcttctc tgctctggct gaaggtctgc aggaagccct cggacaactg ggcggagtgc
      241 cggtagaaca taaaacggac agcctgaggg cagcatggaa acaacagggc gaagatggac
      301 gccgcgagct gactgagcgt tatgctgctc tctgtcagca ctacggaatg cagggcgtac
      361 acaataatgc cggtcggggc cacgaaaatg gctcggttga aagtgcccac ggacatctga
      421 aaaggcgtat ctgtcaggcg ctgatactgc ggggcagtaa cgacttcagc accatagaag
      481 aatatcaggc cttcatcact cagcaggtta tgcggcacaa ccgtaacaat caggatctgg
      541 tcaaggaaga acgtcttcat ctgaaaccgc tgccgcttcg tcgcagtgct gactatgatg
      601 agctgactgt gagggttagc cgcagcagta ccatcaatgt gaagcacgtc gtctacagcg
      661 taccttcccg gcttgtaggt caactgttac gggtccggtt atgggacgat cgtctgagct
      721 gttacgttgg cagcagcgag gtcatgagct gcccacgtgt cagaccagaa aaagggaaga
      781 cgcgggcccg tcgtatcgac ttccgacatg tgatcgacag tctggcaaaa aagcccggtg
      841 cgttctgcca tgcaacgctg agaaatgaca tcctgccaga cgatgaatgg cggaggctgt
      901 ggcgtcgctt atgtaatcat ctggaacccg acatggcagg caggctgatg gtacatgctc
      961 tgaaactggc tgcaggatac gacgatatct cagtcgtggc aaaaggtatg gagcagatgc
     1021 tgaatacccc gggaaacgtg gatctgcacc ggctgatgcg cttcctgggt ataaaggaaa
     1081 aggcgttgcc ggtagtcaat gtgaaacagc ataacctgag cagttatgag caactactgc
     1141 gtggcaaggg aggttcgcag tgagcaatat ccatcacctt gaacgcagcc tgcgtaaact
     1201 acgcctgaca cgagttggag ctgaatggca cgctctggaa aaacgagcac tggcagaagg
     1261 ctggacacca tcgcgctatc ttctgacgct atgcaatgaa gaactcctgt ggcgcgagag
     1321 tgaaaaactg cgtcgttata aaaaggaggc ccggttgcca gttgccaaaa cgctaagcga
     1381 atacgacttc agtcaggtgc cggaactgaa tggagctcag ttccggcaac tctgtgaaac
     1441 gacagactgg gttgatgcag gagaaaacgt tctgctgttc ggagccagcg ggttggggaa
     1501 aagccatctg gcggcagcga tcgtggatgg cgtagtaggc cagggctacc gggcccggtt
     1561 ctacagcgca ggagagttgt tgcaggaact acgtaaagcc agagcgcagt tgaaactgaa
     1621 tgagctgcta ctgaaactgg atcgctaccg ggtgatagtg gtggatgatc ttggctatgt
     1681 caaacgcgac agcgccgaaa cgggagtact gttcgagtta atagcgcatc gctatgaacg
     1741 tgggagcctg gtgataacca gtaaccatcc gttcagcatg tggggcagca tcttcgtgga
     1801 tgagactatg gcggtggcgg cggcagaccg gctgatccat cacggatata tgttcgaact
     1861 gaaaggtgaa agctacagga aaaagacagc gaaggcagta acaagcgcg
//
LOCUS       DAZFRE010000258         1864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFRE010000258 DAZFRE010000000
VERSION     DAZFRE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.264"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..330
                     /locus_tag="ACHKUI_005186"
     CDS             <1..330
                     /locus_tag="ACHKUI_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6601171.1"
                     /translation="ASVYTLAKKMMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGE
                     NWFDRTVSADAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECN
                     AIYAALD"
     gene            377..799
                     /locus_tag="ACHKUI_005187"
     CDS             377..799
                     /locus_tag="ACHKUI_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6601172.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            796..987
                     /locus_tag="ACHKUI_005188"
     CDS             796..987
                     /locus_tag="ACHKUI_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601173.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(1183..1437)
                     /locus_tag="ACHKUI_005189"
                     /pseudo
     CDS             complement(1183..1437)
                     /locus_tag="ACHKUI_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          410 a          495 c          560 g          399 t
ORIGIN      
        1 gcatctgtgt acacgctggc gaaaaaaatg atgccggaat atgaaggcgg attctggcac
       61 ttcatccgcc tgccggacgg tggcggttac atgatgccgg atggcgaccg ttttcatctg
      121 gttaacggtg aaaactggtt tgaccgtacc gtgagtgctg atgccgcagg catcatcctt
      181 acctcccttg tgattaaccg ccagttgtgg ctgtaccacg acagcgggga tgcagggttg
      241 acacacctgt acaggatgcg cgatgcgcag ttgtggagcc acatcgaatt tcaccccgaa
      301 tgcaacgcga tttacgcggc acttgactga ttaacggacg gggcggcaat gccgcccctg
      361 taaaggagac gagaacatgt actgtactgt taaagaaatc atccgcgatg tactggatac
      421 agacgtgccg gacagtgaat gcgtttttgc cgtggtgctg acccgtgggg atgtgcgtca
      481 catagcccag gactggagtc tgacagacga tgagctggaa accgtcatgc agcggctgga
      541 cgatgctttt gtatatggcg catgtgacag agttgtttcc gatattgtaa atgaactgat
      601 ggaagaaaag cgcgccagcc gtcatgtgac agtcccggcg gtgatgctgg aaaaagtgat
      661 ggcgctggca ggcagtgaaa tgaagcgcct gtatgccgtc gggagtgaga acgggggcga
      721 cggtgacgca ttcgtcaggg aggaacgcga agcaatggac gttgtgttac aggcgctgga
      781 cggggagcac atgtcatgaa tatcagcaca gaaacccgcg aaattctgcg taattacagg
      841 gccgtgatta atgcgcggcg tcgtgaaatg gggcagaaac cgctcaccac cgcgcagatt
      901 gttgatgaaa tctgcgattt tgtggcgaat cagcaggcgg ttttcctggg cggtcactac
      961 atcttacagg gcagcagaaa caggtgatac aggggcaggc ggtggaggcc gcctgtcagc
     1021 ccgcgcgaac ccccctggca ggctggcgcc tgccggaagg gtaacgggcc acgctggcgc
     1081 gtcgcccgtt acccttcccg tttgtgatgt gtccggaatg ttttttcctg atgttatgtt
     1141 tctgtggtta ctgttcttct tcttcctctt ctttccgttc ctctcctgtt tatggctttc
     1201 ccgggcgtcc cccaggcggg gcgggctgtc gtgaacggag ccagcggagc tgtctccgcc
     1261 ctggcgggct tccatccctg acgcaaaacc cccggcttgc agcgaccgtt ccggtcgcgc
     1321 cgcccctgtc gcgccgtggc ctgcgctcct ggcatcccgg caaaacgacg ccgggatgta
     1381 aggcgcaacg ccgtttcgcg cacgcgctct ccgtcgttgc ggcgggtccc tgctcattgc
     1441 ctgccgggtg ccacacaccc gtatgagtcc ctgtcgccgg gggcatagac agccacaacc
     1501 tgccgcactg actgtccagg gtaaatgaac agcccacaaa attttttacc caaaacccgg
     1561 taaaaaattt tgcgttctgc ccaggacagg tgcgtcaggc cgtggcagtg atgccccttg
     1621 cggcgaaggt tctcaaacgg gtgaagttca taccacaggg aatacagcag caggttcccg
     1681 cgaaacgtca aaaccgcggc tcccaaaact caacgggtga tgttctttaa aaatcaggat
     1741 ttatttcagg ctccggcaat acgcatttat gtgttctgtc agtcatccgg ctggcaggta
     1801 atgagaacgc aggaacagaa ggaggaaaac accggaaaac gtcagatgca gatatgaaaa
     1861 aagc
//
LOCUS       DAZFRE010000259         1849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFRE010000259 DAZFRE010000000
VERSION     DAZFRE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.265"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            211..405
                     /locus_tag="ACHKUI_005190"
     CDS             211..405
                     /locus_tag="ACHKUI_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601174.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            413..1825
                     /gene="pykF"
                     /locus_tag="ACHKUI_005191"
     CDS             413..1825
                     /gene="pykF"
                     /locus_tag="ACHKUI_005191"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6601175.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          500 a          461 c          430 g          458 t
ORIGIN      
        1 tttcacttat cctcacactg acaaccccgg caccagacgt tgcgcaaaca acgaagtttt
       61 tgcgtaacct tttccctgga acgctgaatc tttgataaca atttattgtc ttacaagttg
      121 tatatttttt gaaacgctgt ttttgttttc cttttggatt aatttcagcg tataatgcgc
      181 gccaattgac tcttgaatgg tttcagcact ttggactgta gaactcaacg actcaaaaac
      241 aggcactcac gttgggctga gacacaagca cacattcctc tgcacgcttt ttcgatgtca
      301 cctatcctta gagcgaggca caaccacttt cgtaataccg gattcgcttt ccggcagtgc
      361 gcccagaaag caagtttctc ccatccttct caacttaaag actaagactg tcatgaaaaa
      421 gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct gaagagatgt tagctaaaat
      481 gctggacgct ggcatgaacg ttatgcgtct gaacttctct catggtgact atgcagaaca
      541 cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa actggtaaaa ccgccgctat
      601 cctgcttgat accaaaggtc cagaaatccg caccatgaaa ctggaaggcg gtaacgacgt
      661 ttctctgaaa gcgggtcaga cctttacttt cactactgat aaatctgtta tcggcaacag
      721 cgaaatggtt gcggtaacgt atgaaggttt cactactgac ctgtctgttg gcaacaccgt
      781 actggttgac gacggtctga tcggtatgga agttaccgcc attgaaggta acaaagttat
      841 ctgtaaagtg ctgaacaacg gcgacctggg cgaaaacaaa ggtgtgaacc tgcctggcgt
      901 ttccattgcc ctgccagcac tggctgaaaa agacaaacag gacctgatct ttggttgcga
      961 acaaggcgta gactttgttg ctgcttcctt tattcgtaag cgttctgacg ttatcgaaat
     1021 ccgtgagcac ctgaaagcgc acggcggcga aaacatccac atcatctcca aaatcgaaaa
     1081 ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc tctgacggca tcatggttgc
     1141 gcgtggcgac ctgggtgtag aaatcccggt agaagaagtt atcttcgccc agaagatgat
     1201 gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact gcgacccaga tgctggattc
     1261 catgatcaaa aacccacgcc cgactcgcgc agaagccggt gacgttgcaa acgccatcct
     1321 cgacggtact gacgcagtga tgctgtctgg tgaatccgca aaaggtaaat acccgctgga
     1381 agcggtttct atcatggcga ccatctgcga acgtaccgac cgcgtgatga acagccgtct
     1441 cgagttcaac aatgacaacc gtaaactgcg tattaccgaa gcggtatgcc gtggtgccgt
     1501 tgaaactgct gaaaaactgg atgctccact gatcgtggtt gctactcagg gcggtaaatc
     1561 tgctcgcgca gtacgtaaat acttcccgga tgccaccatc ctggcactga ccaccaacga
     1621 aaaaacggct caccagttag tactgagcaa aggtgttgtg ccgcagctgg ttaaagagat
     1681 cacatctact gatgatttct accgtctggg taaagaactg gctctgcaga gcggtctggc
     1741 acacaaaggt gacgtcgtag ttatggtttc tggtgcactg gtaccgagcg gcactactaa
     1801 caccgcatct gttcacgtcc tgtaatattg cttttgtgaa ttaatttgt
//
LOCUS       DAZFRE010000260         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFRE010000260 DAZFRE010000000
VERSION     DAZFRE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.266"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            303..647
                     /locus_tag="ACHKUI_005192"
                     /pseudo
     CDS             303..647
                     /locus_tag="ACHKUI_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            647..1123
                     /locus_tag="ACHKUI_005193"
                     /pseudo
     CDS             647..1123
                     /locus_tag="ACHKUI_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            1362..>1835
                     /locus_tag="ACHKUI_005194"
     CDS             1362..>1835
                     /locus_tag="ACHKUI_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601176.1"
                     /translation="MTIARDSVSGEIVHGHDLREMDDLYIRTTTFECPYDKCRIPATP
                     CSFKEINLNQSYFRYKETHRPGCKIHDPKSKNKCNSKNNKKHNSPPALAISLLKLDVS
                     ERNYDSSGRRIIKQTRNDEKDLGGKVHPVSSSSIKPVVDYYINGDNLYEPLSIPPY"
BASE COUNT          549 a          369 c          447 g          470 t
ORIGIN      
        1 cgataaatct gccatattgc tgtagttcag atcattattc tataatgatc aaaattaagt
       61 cagctaaaaa ttggcagatt tattactcag cattctggta ccggttgatg tgggtaaata
      121 ccggaaaggc ggtagcgtgc cattgcaaaa aatggtggga ggtatcgtca ggaggctggt
      181 aattgttggt atggcatgaa taaaaggttt ttcgtttgac tggaaaatta tgttgcagga
      241 acgtgagggc tgaggaacaa agcgtattcc ggcgctgtcc gtccttgctg tcactgacac
      301 tgatgtctgc tttattatcg cctttatctc ttcaggcagc ggatgtccgg cgtagcggag
      361 atgaagcatt tatcattcag cagcagcgtc aggaagccct tgagcaacaa ctgatgcctt
      421 cagcccctga tgttcgcctt tctgcacctg gctcttttgc acgtaagatt aattttcctg
      481 ttgaaacgcc ctgttttcag attaaacaga cggaactgga gggggctgat gcgttaccac
      541 actggctgcc tttacaaaaa atcgccaacg gggcggtcgg gcactgtctg ggggcgaaag
      601 gaattaatct gctgatgagt acattgcaga accgtctggt cgatcatggt taaccctttc
      661 cggtcagcag ataacgcgac tgacagaatt acccccggca tataacctgc agcgcagtgc
      721 gcagttgctg cagcaactga tggtactttt ccccgacaat ccccatgttc aggaaatggt
      781 ggataactgg cagaagagcg taaggagtcg tgcgctcccg gaagaggcga tgacgggctg
      841 gaatgaaggc atgacccgct tacagcagct ggctgaacgc ctgaaccgtc tggatgaaca
      901 gcggggaaaa tacatgacgg tcagtgaact gagaacggag gtgtttggca tcatgcaggc
      961 ttttaaccgg catatcccgg cggaagagca gttacgtcgc tatgatgaag cccgtaacca
     1021 gaatggcagt gaacagcagc aaaaacaggc tgaaatggca ctgaatcagc tgattaatcg
     1081 ttatcaggtg gaacacgctg gcaaaccgga gaggcagcca tgatggctga taaaacatgg
     1141 taatggaaaa taaacccaaa agaatgcgca caccatgggc aatcgaagat attcgctacc
     1201 ttgaaacgca ttatggcgtg aaaaaaaacg cgaaatagcg gcatattgcc atgggagggg
     1261 atatgtctgt caggctgact tgtttttctc ttaaatagcc tttatttaaa ttagccttgg
     1321 tatgaaatta gaatctatta atttttatca aaggagattg tgtgacaata gcaagagatt
     1381 ctgttagtgg tgaaattgtt catggtcatg atcttcggga aatggatgat ctttatatac
     1441 gaacaaccac ttttgaatgc ccgtatgaca aatgtaggat acccgctact ccatgtagct
     1501 tcaaagaaat aaatttaaat caatcatact ttcgatataa agagactcat aggccgggat
     1561 gtaagattca tgatcctaag agtaaaaata agtgtaactc aaagaataat aaaaagcata
     1621 attcaccacc ggcattagct atatctcttc tgaaacttga tgtgtctgaa agaaattacg
     1681 actcttctgg cagaagaatt atcaaacaga cgcgaaatga tgaaaaagat ttgggaggta
     1741 aagtacaccc tgtgtcatct tcaagcatca aaccggtagt agactactac attaatggag
     1801 ataatctcta tgaaccatta tcaattcccc cttat
//
LOCUS       DAZFRE010000261         1820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFRE010000261 DAZFRE010000000
VERSION     DAZFRE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.267"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1771)
                     /gene="tssD"
                     /locus_tag="ACHKUI_005195"
     CDS             complement(<1..1771)
                     /gene="tssD"
                     /locus_tag="ACHKUI_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6601177.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHSK
                     PQVYGVHTGEGTPYENVRLANMLWNEERQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFKIVTPRLHDEIH"
BASE COUNT          480 a          457 c          406 g          477 t
ORIGIN      
        1 agtgaatttc atcatgtagt cggggcgtaa caatcttaaa attatcaata tcatacactc
       61 caccaccatg ttctatagca actacatgat gtatctggaa tttcttaact atctcatttg
      121 gtccgtaata atacccctcc tcagggacat agggggaata tccttgacta acgttattct
      181 gattactttt tataaactgc gccaataaca ctgagtcttt actcacctcc agccacagcg
      241 cctcgcgaaa ctcatcaaag gttttaaact cccgtccgcg caatttatca gcaatatgcg
      301 ccgggattgg tgcgccgttg ccgttattgg ataccgacat atccagccag cgagttcctg
      361 gtgaaagcgg gacgccgtgc cctgttacaa caccaggcag ctttcgcgga tcctccttca
      421 ggtaaacata gataggctta ataccggatc ccgccggaaa aaccagaata taatcgttga
      481 attctttcgg atcaggaacc gggaatggcg gcgtggtata ggtatctgta ccgtccggaa
      541 tcggcgtcac cagaatggtg ggctgatcca gcggcggcct gtcgttaccg gtatggcccg
      601 gaacattccc atgttccgga ttttccggcg tccaggtaat cagggggcca tcgacatcgt
      661 gagcgggggt aaattcataa cgctgcctct cctcgttcca cagcatgttc gccaagcgga
      721 cgttttcata cggcgtacct tcacccgtat gaacaccata tacctggggc ttgctgtgaa
      781 tgtcagttcc ccagaaaaaa cgcacccggg tagtggcggt actgcctgca agcgctttct
      841 ggcgcagttc ttcctcatac cagaactcat cacgtccagg cacacgagca ctgccctcac
      901 ccgcagaggg aatatacaga gcagatgcca acgcaccgat gtaagagccg cgggttgcac
      961 tgagtacgcc actgagcgac cattttcccc atatttgagt cgccaacaca ctactcggga
     1021 acagtagcat ggtttgagct tctgcgggtt ccccgataac gttagcctcg ggatggtggt
     1081 atccacagca gtcatcaacc actggctgag cgaaaatcgc tacttgaccg aagttttcag
     1141 cgggttcttc tgccgttccg gcatctgtac atcccttcac cttcagacag gatttggcat
     1201 agaccgggtt aaccgctggt gaagagagtg gaaggttaac aactggtagc ggttcaggct
     1261 taagattact ggcaggtgga cttttaggcg gaataaataa gctattatag ccagcaggaa
     1321 aggccaacca gtcaaattct gtattggcta tgagatgttt acataatata tagtcataat
     1381 cgacagttat acattcggta ggaagattat tgtcagaaat gtttacatga atagcagtta
     1441 atgaagcatt tcttagctga atataataat accgttccca gcgcccgctt ttatttatac
     1501 gatacaaatc gatttcaata aataaccgct cattctgatt gatggcatta gacagtagcg
     1561 gtgagctttt atcaatgagt ttgcaaaatt gtagcccctg tagattaacg cctttaccag
     1621 tactggtaat ggcgttggta agtgcaaagg caaaaatttc atcttcatgg ccaacctgcc
     1681 agcgattacc aaccgacgca tacgtcccac agcctgaaga aatatcgccc tgcttctcac
     1741 cgattattct caggtaaaca atatcgccca ctttatttca ttcccgttta tccttaactt
     1801 ttttgcatta aatagaaaac
//
LOCUS       DAZFRE010000262         1806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFRE010000262 DAZFRE010000000
VERSION     DAZFRE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.268"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..176)
                     /locus_tag="ACHKUI_005196"
     CDS             complement(<1..176)
                     /locus_tag="ACHKUI_005196"
                     /inference="COORDINATES: protein motif:HMM:NF017333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HFO6601178.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPD"
     gene            complement(284..931)
                     /locus_tag="ACHKUI_005197"
     CDS             complement(284..931)
                     /locus_tag="ACHKUI_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFO6601179.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(1125..1508)
                     /gene="traM"
                     /locus_tag="ACHKUI_005198"
     CDS             complement(1125..1508)
                     /gene="traM"
                     /locus_tag="ACHKUI_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO6601180.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVHEAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIRDKVSSEMERFFPENDEE"
BASE COUNT          594 a          382 c          254 g          576 t
ORIGIN      
        1 aaatctggat aacgttgcaa atgatcctgc aacctcacca atgcttcgtt tgttttcgtt
       61 cttccactac gctctctggc tccaagtaat aatttattcg tttcctcatc aagggtaagt
      121 aaaacttttc tacttggcgc agatcttaaa gctcctttaa tcaccctccc tcccataaat
      181 aaaagagatt tagtcaaaat attcacataa gacgcacaca aaaataaaca acaaaatagt
      241 aaattacgtt aacaaaaacc ggcactttta gtgccgggaa tgcttacatt tctttacagt
      301 gaaggctgtt aatattacta gcgaagtatt gatatagata aaataacaca tcagagatta
      361 tagcatatcg caaccactca tccctgtctg agaaaccata gtgtttttta aaattcataa
      421 atattttttt agattggtca acagagatat ccattctctg agaagaatac tcatgactat
      481 aacccaaaag atacggtatt atcttattaa agcgatattt gtcaggttca caactaaact
      541 ccttgccctt tataaaaaag ccactaacta cgtatctttc aaagaatctc cagacaaaca
      601 aattcaagtt attcacttcc gtactttcaa ccaccacatc ccatgataca tcattcaata
      661 ttacaccatt aataatactc actccattcg aggacgagaa agcatcaact tcagcagtat
      721 gaaataaaat ttgcgtatca atatcaatac cagaaaacca tatttcagaa tcactattct
      781 gacactttat taagtgtaaa aaaacagagt ttgagtctat gaattttcct gactctgtgc
      841 ggacacagac tggatatttt gcggataaaa taatttccgg caggcctgat tgacgtatga
      901 gtgctctttc tctagtatcc atcgcacaca taggaacctc ctcaaaggat tctatggaca
      961 gtcgatgcag ggagggacaa gctccctgca tcggcgattt tcaagttcaa attttaacgt
     1021 ggcattaacc acgtaattaa tataaccttt atgcggttaa ttgtcatcaa attgaaccag
     1081 atcaaaatcc tgttatttga agcaaatcaa cttttaattc ctttttattc ctcatcattt
     1141 tctggaaaga acctctccat ctcagatgac accttatccc tgatatcttc gaccatattc
     1201 gcatattcaa actttggatt cccggaaaca tgagggctta gtgactcaat acctaaaatc
     1261 ttagcaactg ttgactgtgt ttttaccgca cattcaagaa gtaccttatt aaactcagcc
     1321 tgattgaacg ctgactcttt acgctccatc tgagcctcat gtacacgaag ccctaattcg
     1381 agaagcatag tggatatgct tgagaaactt acatctgtac ttttagctcc ttctgcacgt
     1441 cttctttcca caattttatt tattttatag acaatttcgt cactgacata agcctgcact
     1501 ttcgccatat gtatacctct ttattttctt atcgcatcaa atttatttca catataaaag
     1561 aatgacaatt catcgatgaa tcgaatcgtg acgcaagtta cgaaaattac gattcatgca
     1621 gcaaatcaca tcacgaattg aatctagagt caattcgctt taactcgttg ttttattatt
     1681 actgattcat ctatgagttg cgttaaattc attggtgaat catatgcgat tcaccaatga
     1741 atccttttta taatgtaaaa taaattaaaa tacattattt aaaacataag ttaatgattc
     1801 aaatag
//
LOCUS       DAZFRE010000263         1789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFRE010000263 DAZFRE010000000
VERSION     DAZFRE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.269"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..189
                     /locus_tag="ACHKUI_005199"
     CDS             <1..189
                     /locus_tag="ACHKUI_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO6601181.1"
                     /translation="PSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     MMSHSHQSDTRDLLKSVG"
     gene            211..894
                     /locus_tag="ACHKUI_005200"
                     /pseudo
     CDS             211..894
                     /locus_tag="ACHKUI_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            901..1692
                     /locus_tag="ACHKUI_005201"
     CDS             901..1692
                     /locus_tag="ACHKUI_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO6601182.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
BASE COUNT          514 a          392 c          428 g          455 t
ORIGIN      
        1 ccaagcttca ttgccagcca gatgggccat gcaagtgccc agatggtttt caatgtatac
       61 ggagcatgga tgactgacag caatgcagaa cagatcgcaa tgctgaatca gaagctggca
      121 gattatgtcc caatgatgtc ccatagtcac caaagtgaca caagagacct attaaaatca
      181 gtgggttagt ctttaatacc cgtcatgtta actgcattcc gtgcccccac aatggcagaa
      241 atgtacaggg atgatgtaca tttttaccgc aagggtaaac ccaattactg ggtacctaac
      301 cttaatctga aaccagaaaa taacattacc cgtgagattg gcgcaggtat tcaactggac
      361 ggcctgctta caggcaatga tcggctgcag ttgaaaggcg gatatttcgg aacggatgcc
      421 agaaactata ttgccacgcg cgtggatatg aaacggatgc gttcttattc ttataatgta
      481 tcccgggccc gtatctgggg atgggatatg cagggaaatt accagtctga ttattttgac
      541 tggatgcttt cttataaccg cacggaaagt atggatgcca gcagcaggga atggctgggc
      601 tccggcaatc ctgacacact catcagtgac ataagtatac ctgttggtca tggaggagtt
      661 tctgccggat ggcgtgctga actttcggca gcagccacgc atgtgaaaaa aggcgatccc
      721 catcaggctg gctatgccgt acattccttt tcactgtctt ataagcctgt aagtgttaaa
      781 ggctttgagg catcagtgac tctggataat gccttcaaca agcttgccat gaatggcaaa
      841 ggagtgccgc tttcaggcag aactgtcagt ctttataccc gttatcagtg gtaacaaatc
      901 atgatgaaaa atacaggtta tatcttagct ctttgtctga cagcatcggg gcatgtcctg
      961 gcccacgatg tctggattac aggtaaacag gcagagaaca acattaccgc agagattggt
     1021 tatggtcata atttcccctc aaagggcaca attcctgaca gaaggaattt ctttgaaaat
     1081 ccccggattt ataacgggaa agagacaata acactgaagc cagcgtccac ggattatgtc
     1141 tataaaactg agtctgcaag caaagataat ggctacgttt tgtcaacata tatgaaaccg
     1201 ggatactggt cgagaacctc ttcaggatgg aaaccggtca gccgggaggg cagaaatgat
     1261 gtggcttact gtgagtttgt cactaaatat gcgaaatctt ttattcctgg tgaacagcag
     1321 atgccagcac aactctatca gtctccaacg gggcatgagc ttgaaatcat tccgttatcc
     1381 gatataagtc gtttcagtga agatgtgaag ctgaaagtcc tgtataaaac gtccccgctc
     1441 gccggagcta tcatggagct tgactcggtc agttatctga aatcatcccg tcatactcat
     1501 gcagttgagc acaaacatcc tgttcataaa gcagaactca cctttgtaac taatgaggat
     1561 ggtatcgtca cagtaccttc tcttcatatt ggacagtggc tggcgaaagt ccaaaataag
     1621 aaaagttttc aggacaaaag cctgtgtgat gaaactgtcg atgtggcaac tttaagtttc
     1681 tcccgaaatt aacaatacta ttccctgccc tgaaattgta agcgtgcagc aagaaccgta
     1741 ttgacgggga tgtgttattc agtcggcagt gctacgcgcc aggggagca
//
LOCUS       DAZFRE010000264         1784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFRE010000264 DAZFRE010000000
VERSION     DAZFRE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.270"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..503)
                     /locus_tag="ACHKUI_005202"
     CDS             complement(<1..503)
                     /locus_tag="ACHKUI_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6601183.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKAKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQE"
     gene            complement(567..917)
                     /locus_tag="ACHKUI_005203"
     CDS             complement(567..917)
                     /locus_tag="ACHKUI_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6601184.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEVGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGNEPLPEKRPVILEAQNVWITYKGNLPG
                     RITGSLKTPPESQP"
     gene            complement(922..1137)
                     /locus_tag="ACHKUI_005204"
     CDS             complement(922..1137)
                     /locus_tag="ACHKUI_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6601185.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1214..1459)
                     /locus_tag="ACHKUI_005205"
     CDS             complement(1214..1459)
                     /locus_tag="ACHKUI_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6601186.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            complement(1497..1679)
                     /locus_tag="ACHKUI_005206"
     CDS             complement(1497..1679)
                     /locus_tag="ACHKUI_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6601187.1"
                     /translation="MTFIHQVMLYFCTAVCVMYLLSGGYRAVRDFWRRQIDKRAAERI
                     SASQSAGSKPEDPLIP"
BASE COUNT          410 a          523 c          389 g          462 t
ORIGIN      
        1 ctctcctggt cgcgacggga tacctgaccg taacagttgt ttgaacgaat acggcagtct
       61 ctgccaccgt ccttaatcca ccagcgaatc gcttcgcagg caccttttcg atcacctgca
      121 ttaattcgtc tgtaaaacgt cgacgggaaa cacttaccgg gaccaatgtt gtacggacag
      181 aatgacgcga tccccgcttt ctggggttcg gtcagtggca ctctgatgtt tttctccacc
      241 catgccagcg ccttatcacg ctcaatggcg ttaacccggt cgcattttgc cttcgacaac
      301 ttcatgcctg gaacaacagg tttaccatcc accatgatgg caccacggca gatggtccag
      361 atccccgcac catcacggta tgccgtggtg tggttacctt ccttttcatc cagaaactgg
      421 tcgagaatgt caggcgcagg cgcaccagcg gcaatcagcg ccagaacggc agccgacagg
      481 ccgtatctga tttttgcgtt catggatatt tatcaggatt tatcggtttc tgcccacgga
      541 caggtttatc tgttccggtc agtgaattaa ggttgtgatt ccggtggagt cttcagagaa
      601 ccagtaattc ttcccggtag atttcctttg taggttatcc atacattctg cgcctctaaa
      661 atcacggggc gcttttccgg caacggttcg ttcccttcac ataacccggc agcaacatcc
      721 atgaaaaact gcttcgcctg ctttttcgcc tcagcttcgt aaaactccag cgtggcacct
      781 tcagtacggt caagactaat cgccacatct ggcaacaaca gtgacggata cccaccaact
      841 tccagtgcca cagtaacagt aatcttatcc gggtaattat ttatcccttt aacaaccagt
      901 tcgtattttt tcttcatcgc tttactctcc ccgcgccgcc ttacgccggt cctctctgat
      961 tttgaaatac aggttagtca gatatgtcag cagcccaaac agcagactcc ccagcacgcc
     1021 tattgccgcc cactgagacg gggaaaccct gtccagcaac tgcaggaacc agtagcccgt
     1081 tcccaccgct gacgtggtgt atgacacacc tgttgtgatt ttttccatct ggtacatacc
     1141 ccgtctcccg ttatccggaa gctcacaaca atataaagac caccggcaca caccgatggt
     1201 cccttgcgca ggcttacatc atcatgtcgc tgtcaggtgt gggttcaccg ccatctgaag
     1261 cactcccgtc acccgcgata ccttccggct ccggaaccgc tggtacgccc agcagctcat
     1321 ccagaatggc atccacttct gcatcaagac gcgcctcaag gttctggcgg agtttctgtt
     1381 tcagtgcact ccggacttct tcagagcgca ggacttcctt cactgcctca gcagtgacca
     1441 gggatgtgat ttctgacatg ggattttctc gttgaaaggt gttgtcaaga aagtgactac
     1501 ggaatgagcg gatcttcggg tttgcttccg gctgactgac tggcgctgat tctctcagcg
     1561 gcccttttat caatctgcct gcgccagaaa tcgcgcactg ccctgtaccc acccgaaaga
     1621 agatacataa cacagactgc cgtacagaag tacagcatca cctgatgaat aaatgtcata
     1681 atttcttacc gttatggttg acaatgagaa ctgttttcat ttaaaaaacc gatatacgaa
     1741 agcatctttt ctttacattc tccattggaa ttacctccgc cagc
//
LOCUS       DAZFRE010000265         1783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFRE010000265 DAZFRE010000000
VERSION     DAZFRE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.271"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..458)
                     /gene="mobF"
                     /locus_tag="ACHKUI_005207"
     CDS             complement(<1..458)
                     /gene="mobF"
                     /locus_tag="ACHKUI_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFO6601188.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQ"
     gene            complement(458..>1783)
                     /locus_tag="ACHKUI_005208"
     CDS             complement(458..>1783)
                     /locus_tag="ACHKUI_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6601189.1"
                     /translation="EDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLR
                     TFLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVRE
                     DQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKL
                     PDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAE
                     FAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTL
                     PGPYPAVKLSLKYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEV
                     ASGEDVTQAEQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISE
                     SGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          394 a          534 c          435 g          420 t
ORIGIN      
        1 tcctgatcgc gactggtgtc atggttaaac agtgccatca ccagattacc ggtcagcacc
       61 gtttctgact gtccgtccgt catcacccgt gtggaagcca gcgcctccac ctgacggaca
      121 gcaaaatcca cggcctggtt atgtgcatca ataagacgtt tatccccccc gagcatggcc
      181 atcatggaga cacttttggg ggcggagaag gtcaggtcgt agccgggacg atgcttatta
      241 ctgccatcct gcatgcggct taaatccgcc ccgtccggca gcctgccctc cagaagacgg
      301 gtaaaaacat ccttatcgac gctgccctgc agcccaagct gttcagcccc ctgaccagcc
      361 cagcgttctc ccatgctgcc cagcacatag taattatcct tgtcggtgta atagtttccg
      421 gcactgcctg ccgatttaac aacgctgaaa ctcaacatca gaaatcatct cccggctcaa
      481 catcctcccc gcgctcccgg tgcacattaa tgttcacctc ttcacgacgc tgcatatgct
      541 gctggatgtc cggatgattt tcctgttgcc atgcctcata agcggccata tccaccactt
      601 caccggattc actgatcccg ggtggcagtt gctgttccat ctcttcttcc ggtttcattt
      661 tcagctcctg ctcgatcccc cctgccggaa cactcacacc tgcatctgac ttcttatcat
      721 tgatgacaga agacacaggc tgttgtggct gttcagcctg agtcacatct tctccggagg
      781 caacctccgg ttcaaagagg ctggccatct gacgaccttc tgcttccctt gcggcaagta
      841 cggcactcag acggttctcc atttccgggt tgatgtcacg cggaataaac tccggagcga
      901 ccttcggtcg tgcctgatat ttcagagaga gtttgactgc cggatacggt ccgggcagag
      961 tgacatagca ggtcagatcc ggcagagact gaatgtcgga ataactgacc agcgtctgac
     1021 gctccatatc cttaccggtc gataccccgt cacgtaccgg gtcagcaccg taggaatact
     1081 gctcgctggc tttcaggtgc tctttctcac caatctcacc ggcagcgaac tctgcaatct
     1141 tatggctcgg ggaacggaaa aaagcacggg tgttcatgac gtcaaacagc gtggccgccg
     1201 ctttctcacc gtagatatct tccagctggg cataggactg gataccaaac acataacagc
     1261 caccgaactt acgggcttcc ggcaggatct ccaccagatc cggcagtttg tgtaacgtgg
     1321 gtaactcatc acagaaaaac cacacacgac ggttacggtt ttctcccatt gccagcagac
     1381 cacgaatggc aatggacagc cacatggaga tcacaggctt cagggaggca tgggtgtcgg
     1441 cgttcgacga aataaacagc cagccgtttt tctgatcttc ccggacacca cgcatccagt
     1501 cacggatggt gaagggatca ccgttatgct caatcccctg caggtaacgg atggctttca
     1561 cgtagttggt cagcacagca cggatggaaa tcgccgtttt ctcaattttc tcttccacca
     1621 ggttggccgc cggtgaatta cgcaggaagg tacgcagttt ttcaattttg atggaaagca
     1681 gtgtgtccac cagtttgctg tagctgcggt tggggtcatt acgcatcagg tacgccgctt
     1741 ccgcaaaaat ggtacgtcct gaaccctgcc agaacgggtc ctc
//
LOCUS       DAZFRE010000266         1781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFRE010000266 DAZFRE010000000
VERSION     DAZFRE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.272"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            241..912
                     /locus_tag="ACHKUI_005209"
     CDS             241..912
                     /locus_tag="ACHKUI_005209"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6601190.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETLSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            922..>1781
                     /locus_tag="ACHKUI_005210"
     CDS             922..>1781
                     /locus_tag="ACHKUI_005210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601191.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVG"
BASE COUNT          474 a          400 c          416 g          491 t
ORIGIN      
        1 taaatcacat ttaaatacaa tatataaaac aaaataacca tacaaaccaa acacacaaac
       61 acttaacata tgaactacat aaaagattac acactcttat ccccgtacct gacagcccct
      121 tgtttttcag ctatcagcta tttttaacac ttaaaaatta gcatctgtct gcgtcagagg
      181 ttacgtctct gggggcgtcg ttgtgcttgt agtataataa ataaaatgga gtgtcataaa
      241 gtggataagg ttttagattc agcgatcctt tcttcggcaa ataaaagaaa gggtatcctt
      301 gccattggcg cgcatcctga cgatatagaa ttaggctgtg gcgcatcgct agctcgcctt
      361 gcgcaaaaag gaatttatat tgctaccgtg gtgatgacta ccggtaattc tggcgtagat
      421 ggaataatcg atcgccacga agaatcacgc aacgctctaa agatattagg gtgccaccaa
      481 actattcatc ttaattttgc tgacacccgc gctcatttac agctcaatga tatgatttct
      541 gccctggaaa acatcataaa aaatcaaatt ccctctgatg ttgaaattat acgtgtatat
      601 accatgcatg atgctgaccg ccatcaggat catcttgcag tctatcaagc ctcaatggtt
      661 gcctgtcgcg ctattccgca aattctcggc tatgaaacac taagtacctg gctttcattt
      721 atgcctcagg tttttgaatc cgtgaaagaa gaatattttt ctcttaaact gactgcgtta
      781 aaaaaacata aaagtcagag ccagcgagat tatatgcgcc cggagcgtct gcgtgcagtt
      841 gctcaatttc gcgggcaaca ggtcaatagc gatctgggtg aaggttttgt tattcataaa
      901 atgattcttt gagcaaattg catgaaaacc tggatcttta tatgtatggc cgtagcaata
      961 ttgctatggt ttctgagtac gttaaggcgt aagcccagcc aaaagaaagg ctgtattgac
     1021 gccattatac ctgcttataa cgaaggcccg tgtctggcgc agtcactgga taatctgctg
     1081 cgtaaccctt atttttgccg ggtaatttgc gttaacgacg gctccacgga caataccgaa
     1141 gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct ttgttgccgt cacgcaaaaa
     1201 aataccggta aaggtggtgc gctgatgaat ggcctcaatt acgccacctg cgatcaggtt
     1261 tttttaagtg atgccgacac ctatgttccg cccgatcaag acggaatggg ctatatgctg
     1321 gcggaaattg agcgtggcgc tgatgccgta ggcggcattc cctctactgc gttgaaaggc
     1381 gcgggtctgt taccacacat ccgcgcgacc gtaaagttgc cgatgattgt tatgaagcgc
     1441 acgctacagc agcttctggg cggcgcaccg tttatcatca gcggtgcctg cgggatgttc
     1501 cgtactgatg tattgcgtaa gttcggtttc tcggatcgta ctaaagtcga agaccttgat
     1561 ctcacctgga cattggtggc aaacggctat cgtattcggc aggcgaatcg ctgcatcgta
     1621 tacccacagg aatgcaacag cccgcgtgag gaatggcgtc gctggcggcg ttggattgtg
     1681 ggctacgcgg tctgtatgcg cctgcataaa agacttttat ttagccgctt cggtatcttc
     1741 agtatatttc ctatgctgtt ggttgtgctt tatggcgttg g
//
LOCUS       DAZFRE010000267         1770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFRE010000267 DAZFRE010000000
VERSION     DAZFRE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.273"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(166..384)
                     /gene="ymgF"
                     /locus_tag="ACHKUI_005211"
     CDS             complement(166..384)
                     /gene="ymgF"
                     /locus_tag="ACHKUI_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO6601192.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(516..1307)
                     /locus_tag="ACHKUI_005212"
                     /pseudo
     CDS             complement(516..1307)
                     /locus_tag="ACHKUI_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            complement(1488..1736)
                     /gene="ymgC"
                     /locus_tag="ACHKUI_005213"
     CDS             complement(1488..1736)
                     /gene="ymgC"
                     /locus_tag="ACHKUI_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO6601193.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDREFVLS"
BASE COUNT          561 a          389 c          341 g          479 t
ORIGIN      
        1 aaattatcaa cagttgccat ggaaattatt tcactgatat ataaaacaac tgtgataaca
       61 ccagggttga caactatttg tgcaataatt cctttccttc aatgatccag gggttcactg
      121 ccagcaaccc attcagccgc atactggcag tgagaaactg aaagattagt gctgatccct
      181 gtggctgacg agtctttccc ttaatcgatt aaccagattg atcgccagcc aaaccagggc
      241 attaagcaaa tttatcgtca caagcacttc tcccagcccg gtgctgggtt ttgcaataag
      301 gatgaccagg agagtgatca gcataaatgc aatggaaaag atgatataga gagtgaaata
      361 atccagatta ttactattgt tcatgctact aatggcaatc cagtcaaaac tttcacacaa
      421 ttatagcaaa caaaagaatg aacaatagta acaagaggca acgagctaat tagctgtcta
      481 taattacacc gcaccatggc cttgctgatc aattttcact caaccacaac cttcaccttc
      541 ggtttagaaa ggataatttt gaccatgtcg atatatgtaa caggtttata gaaataataa
      601 ccctgctgaa atgtgatatt attttggttc agatagtcaa attgttcttt cgtttcgaca
      661 ccttcagcga cgatactgat cgaaagttta cgcgccagat ccaatacaca atccagaatt
      721 cgggttgatg ctgggtctgc gttgacgcgg ccaacgaaac tatgatcaat tttgatataa
      781 tcaatatgca ggtcatgcag ataagaaagc cctgagtaac cagtaccgaa atcatccagc
      841 gcgatgacaa aaccattttc atgcagaatg ttcaaccgct gaaccagact ttcatcaaca
      901 tttaatggct cacgctcggt gacttccaga acaaggttta aatcgcggcg ggtaaaactg
      961 tcacggtaat ttaagcactc gtcgacaaac gtcggcgaaa taatatgcga ggcgctaaaa
     1021 ttaatcccaa tatgaaaacc ttccggcagt ttactcgcga tagcgttcat ctgtctggca
     1081 acctgattca tcaggctttg cgtaagcgga acgattaatc cgcatttttc agcaagtgga
     1141 ataaatgccg cgggtgatat atatccaccg tgaggttgtt tccagcgggc taacacctca
     1201 actccccgca atgtcccttc ccgaccattt accacaggtt ggtaaaaagg gacgatctcc
     1261 ccattattta tagcccgtcg caggatctct tcaggcgttg cgcttttata ggcagaatta
     1321 aacaaattag gcgggatatc aggcgtcaag aatggaaaac gagcgctctc cattcctgtt
     1381 tgccctctga tattgcggat atattaagaa agcataacgc ctgaaatgct cactttgcat
     1441 cagcatagtg atacagctga tgtttattca aaaaccttac tcaagtttta agagagcaca
     1501 aattccctgt cattaataat gatatccgtt agtgtagtct tatgcatttc aatgtactgc
     1561 aagatttgca gatagagatc agaaagaata acactggtct catcttttaa acgcaagcgt
     1621 aaagcctgag caataatatg actatcaaca tatccatgtg tcatgaacac ctccctctcg
     1681 agatttttaa acaaagcaca ttgaaaaata aatctctctg ggattgaatt attcatgtca
     1741 gcctcgcagg aatagtgatc caataaaatt
//
LOCUS       DAZFRE010000268         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFRE010000268 DAZFRE010000000
VERSION     DAZFRE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.274"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(75..848)
                     /locus_tag="ACHKUI_005214"
     CDS             complement(75..848)
                     /locus_tag="ACHKUI_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6601194.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(850..1443)
                     /locus_tag="ACHKUI_005215"
     CDS             complement(850..1443)
                     /locus_tag="ACHKUI_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6601195.1"
                     /translation="MSQELKNYLKLNAKEKNVQIADQVIIDESAGEVVIGANTRICHG
                     AVIQGPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIAD
                     SVVANQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIIL
                     PGRIISPNTQLGPRVIVERNLPSGTYSLRQELIRTGD"
     gene            complement(1409..>1723)
                     /locus_tag="ACHKUI_005216"
     CDS             complement(1409..>1723)
                     /locus_tag="ACHKUI_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601196.1"
                     /translation="NEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLLVPLA
                     PLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGTEKLS
                     EA"
BASE COUNT          480 a          410 c          391 g          442 t
ORIGIN      
        1 tattgtttat tatcaaaccg agttatagct tttctctgaa aactaccgaa taaaatatac
       61 tgatagactg ctgcctagaa tttccagatc aggtgggctg caagttgcag accgttataa
      121 tcatcggttg gggtgttgaa ccacacttta cctgccgtca acccgagatt aagttgctca
      181 gtcagcggtt gaattcgttg caaactgatg ctcttcatgc tgccgtagcg tgtttctggc
      241 gtccagtcgt aggtgtaagc acctgttccg cggcttagcc atagttgcgt ataaccagca
      301 ccgcgcgggt ccttcagacg cacggaaatc atattgctat aactactacc tgcatcgctg
      361 gagtcataat gggtatagcg gtaactggtg atcaccgggc cagtatagag tgatacgccg
      421 ccttgccagg cattgacttc gacatcatcg taatatttag tgtagcgata accggtcgtg
      481 aaaagcgttt ttggcaacag ggctaggtta atatcctgtc gaaaatcctg gcgggcaaag
      541 accggcgtat tatccgcaaa ggcaataccc gttcgggttg tcagccagtt attccatttg
      601 taccagactg caccctggcc gcgagtggca ttgaaatgtt cagtttcata atcacgacgc
      661 ccctgggaaa gattaaaaag cagcgttgcg ttttcaactt ttgctaccag ttcagcatac
      721 gctaaattac ggttgccatg ttcgccagaa taatcggtaa aatcgtagcc cgcggtaatg
      781 gaagttaacc cggcataagc gggcaatgca ctaaaaaaaa acggcactaa taacgtatgc
      841 tttatcattt taatctcctg tacggataag ttcttgtcgg agtgagtaag ttccactagg
      901 taaattacgt tctacaatca cgcgcgggcc aagttgtgtg ttcggagaaa taattcgccc
      961 aggcaaaata ataacttgta caccaaggcg tgaacgcttc ccgatataac aacctaattt
     1021 atcgcatccg gtagcgataa ttccctctgg agttcgaaca gacacgggtt gttcatccag
     1081 acgatgatta ctggtacgta cttgtgcgcc caaatatgcc tggtttgcaa ctaccgagtc
     1141 ggcaataaag cattgcggac caatcgttgc ttccgcttca ataaccgcat ttttaatttc
     1201 ggtggcaaaa ccaattttta cgccattgct gattattgtg ccaggacgaa taaacgcata
     1261 attacctatc aggcagtttg cgccaatcac taccggcccc tgaataacgg caccgtggca
     1321 aatacgggta ttcgcgccga taacaacctc accggcactt tcatcaataa tcacctggtc
     1381 tgcaatctga acatttttct ctttagcatt aagcttcaga tagtttttca gttcctgtga
     1441 cagaaggttg ggaataagcg gttgacgctt ccaccagtgc ggaatagcgg gtgggtttgt
     1501 cgcgctgagg ttcgcgtcca gtaaaaaagg caataagtcc ataaataatc cagatggcat
     1561 aagctaataa tacatacaca acggaaagcg gtgctaaagg caccaacaac cagcaacgat
     1621 gaaaccaggc gctaaatgca ccaataacac aaactacgcc gacccagata agcgggaaca
     1681 ttgccaacac cacaccatgt ggcccggtgg tgatgaattc att
//
LOCUS       DAZFRE010000269         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFRE010000269 DAZFRE010000000
VERSION     DAZFRE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.275"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..269
                     /locus_tag="ACHKUI_005217"
     CDS             <1..269
                     /locus_tag="ACHKUI_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601197.1"
                     /translation="VNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRKSHPEKMA
                     FSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            388..1209
                     /locus_tag="ACHKUI_005218"
     CDS             388..1209
                     /locus_tag="ACHKUI_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601198.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1504..>1692)
                     /locus_tag="ACHKUI_005219"
     CDS             complement(1504..>1692)
                     /locus_tag="ACHKUI_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFO6601199.1"
                     /translation="GVSWEAVGAYNAGFRKTERQNQRRLAYASEVYRIYTGIKSSKGI
                     RIPVTKKSLPEINSVQNN"
BASE COUNT          446 a          352 c          426 g          468 t
ORIGIN      
        1 acgttaacac cgcgtcgtac acgattcttg ttgccgggag ggaaaaaaac accggggaag
       61 agtgggacgt cctggaattc agcagcctga ctgagctgaa aaaatatcgc aaaagccacc
      121 cggaaaagat ggccttcagc tatagctacg cgctcagccg gggtgtggat acgcagttcc
      181 gccatatcaa cattgcggaa gccgatcact tcaaacagtt cctgcgtcag attaagcgtg
      241 ccggcctcga tatccgggcg atctgctgac cggatgtgat gaatatccct cctgattgtt
      301 gcaatcttgt taatggagat cgggagggat gatattaagc ggaaaaattt tttattaaat
      361 ttattctttc ataaaggagt agctgttatg cgattagctt cccgttttgg tcggtataat
      421 tccatccgtc gtgaacgtcc tttaacggat gatgaattaa tgcagttcgt gccttcggta
      481 ttttccggtg ataaacatga gtcccggagt gaacgttata cgtatattcc aacaatcaat
      541 atcatcaata agttacgtga tgaaggtttc cagccattct ttgcctgtca gagtcgggtt
      601 cgtgatttgg gacgccgcga atacagtaaa catatgttac gtcttcgcag ggaagggcat
      661 attaacgggc aggaagttcc tgaaattatc ctgcttaatt cacatgatgg ttcatccagt
      721 tatcagatga tccccggaat ttttcgtttt gtctgcacaa atggcctggt gtgcggaaat
      781 aattttggcg aaatccgtgt tccacataaa ggtgatattg tcgggcaggt tatcgaggga
      841 gcgtatgaag tgctcggtgt ctttgataag gtcactgata atatggaggc gatgaaagaa
      901 attcatctta acagtgacga gcaacattta tttggcagag ctgcactgat ggtcaggtat
      961 gaagatgaaa ataaaacgcc agtgacgcct gaacaaataa ttactccccg tcgtcgggaa
     1021 gataaacaga acgatctctg gacaacctgg cagcgggttc aggagaatat gataaaaggt
     1081 ggattatcgg ggcgaagtgc ctccgggaaa aataccagga caagagccat tacaggtatt
     1141 gatggtgata taagaatcaa caaggcgtta tgggtgattg ccgaacagtt cagaaagtgg
     1201 aagtcatgat aatataagta aagccccgaa aaattttcgg ggctttactt atattatcat
     1261 tcttcggcat cgctgtgtcg tctgatatac ctcaattcca tcaaccggct ttccggttgt
     1321 ctgtaaatat tgtgcccgcg aaaaatggtc tggcaggagg ctgcgcagtt accgggtata
     1381 tataagatgg attcccgctt cgcggggctt cggccagtca gcattgtcac tcatatgata
     1441 tttttgtgtg tggccttcat gccgcttttg cggcataacc ggtatctgac aatgtctgta
     1501 agattaattg ttctgcacgc tgttaatttc cggaagtgat ttcttcgtga ccggaatccg
     1561 gataccttta ctgcttttta tcccggtata aatccggtaa acctctgatg cgtaggcaag
     1621 acgtctctgg ttctggcgtt cggtcttcct gaatccggca ttgtatgcac caacggcctc
     1681 ccaggagacg cc
//
LOCUS       DAZFRE010000270         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFRE010000270 DAZFRE010000000
VERSION     DAZFRE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.276"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1677
                     /locus_tag="ACHKUI_005220"
     CDS             <1..1677
                     /locus_tag="ACHKUI_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO6601200.1"
                     /translation="ETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNS
                     MIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGI
                     SKDDGLITLEDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVA
                     NSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIAL
                     EGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKP
                     DREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAF
                     DRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQD
                     GVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVL
                     AQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERET
                     ALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
BASE COUNT          388 a          430 c          592 g          282 t
ORIGIN      
        1 gagacttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga ctataccggc
       61 aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga ggaccggcgc
      121 gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gtgagctggg gaaagagcag
      181 gtcatggtgc ctgtcctgaa cacagcgaat atacgtgacg gggagctgcg tcgtctctcc
      241 acctgggaga ataacccgga tgcccttgcc ctggtggata gtgtgtatca ccggattgcc
      301 ggtatcagca aggatgacgg gctgataacc ctggaggatg cggaaggtaa cacgcggctg
      361 atttcgcccc gggaggcggt ggctgaaggt gtcacactgt acaccccgga caaaatccgg
      421 gtggggaccg gcgaccggat gcgcttcacg aagagtgacc gggagcgcgg ttatgtggcc
      481 aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacgctgtc agacggacag
      541 cagacccggg tgattcgccc cggccaggag cgggcagagc aacatattga cctggcttat
      601 gccatcaccg cccacggtgc gcagggggcg agtgaaacct ttgccatcgc gcttgaaggc
      661 acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc cctgtcgcgt
      721 atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga tgccattaac
      781 aatgccgtac agaaaggaac tgcccacgat gtgctggagc cgaaaccgga ccgggaggtc
      841 atgaatgcgc agcggctgtt cagtacggca cgggaactgc gggacgtggc ggcaggccgt
      901 gccgttctcc gtcaggcagg gctggccggg ggagacagcc ctgcacggtt tattgcaccg
      961 ggacgtaaat atccgcagcc gtatgtggca ctgccggcgt ttgaccgtaa cggcaagtca
     1021 gctggtatct ggctgaaccc actgaccacg gatgacggaa acgggctgcg gggattcagt
     1081 ggtgaaggac gggtgaaagg cagcggggat gcgcagttcg tggccctgca gggcagccgt
     1141 aacggagaga gcctgctggc tgataatatg caggatggcg tccggattgc ccgtgataat
     1201 cctgacagcg gtgtggtggt gagaattgcc ggtgaaggtc gtccgtggaa tcccggtgcc
     1261 atcaccggtg gccgcgtgtg gggggatatc ccggataaca gcgtccagcc gggggccgga
     1321 aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga ggccatccgc
     1381 cgtgaaacgg aacgccgcgc agatgaaatt gtcaggaaaa tggcagagaa caaacctgac
     1441 ctgccggatg gcaaaacaga gctggctgtc agggatattg ccggacagga gcgtgaccgg
     1501 tctgccatat ctgaacggga aaccgcactg ccggagagtg tgctgcgtga atcacaacgg
     1561 gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg tctgcagcag
     1621 atggagcggg atatggttcg tgacctgcag aaagagaaaa ccctgggcgg agactgatac
     1681 aggaagacaa ac
//
LOCUS       DAZFRE010000271         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFRE010000271 DAZFRE010000000
VERSION     DAZFRE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.277"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1677
                     /locus_tag="ACHKUI_005221"
     CDS             <1..1677
                     /locus_tag="ACHKUI_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO6601201.1"
                     /translation="EAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNS
                     MIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGI
                     SKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVA
                     NSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIAL
                     EGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKP
                     DREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAF
                     DRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQD
                     GVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVL
                     AQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREA
                     ALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKEKTLGGD"
BASE COUNT          389 a          434 c          590 g          279 t
ORIGIN      
        1 gaggctttcc cggatatccc catgacactg tatgaggcca ttgtccgcga ctataccggc
       61 aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga ggaccggcgc
      121 gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gtgagctggg caaagagcag
      181 gtcatggtgc ctgtcctgaa cacagcgaat atacgtgacg gagaactgcg tcgtctctcc
      241 acctgggaga aaaacccgga tgcccttgcc ctggtggata gtgtgtatca ccggattgcc
      301 ggtatcagca aggatgacgg gctgataacc ctgcaggatg cggaaggtaa cacgcggctg
      361 atttcgcccc gggaggcggt ggctgaaggt gtcacactgt acacaccaga caaaatccgg
      421 gtggggaccg gcgaccggat gcgcttcacg aagagtgacc gggagcgcgg ttatgtggcc
      481 aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacgctatc ggacggacag
      541 cagacccggg tgattcgccc cggccaggag cgggcagagc aacatattga cctggcctat
      601 gccatcactg cccacggtgc gcagggggcg agtgaaacct ttgccatcgc gcttgaaggc
      661 acggaaggta accggaaaca gatggccggc tttgagtcag cctacgtggc cctgtcgcgt
      721 atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga tgccattaac
      781 aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga ccgggaggtc
      841 atgaatgcag agcggctgtt cagtacggca cgggaactgc gggacgtggc ggctggccgt
      901 gctgttctcc gccaggcggg actggccggg ggcgacagtc cggcacggtt tattgcaccg
      961 gggcgcaaat atccgcagcc gtatgtggca ctgcctgctt ttgaccgtaa cggcaagtcc
     1021 gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg gggattcagc
     1081 ggtgaaggac gggtgaaagg cagcggggat gcgcagtttg tggccctgca gggcagccgt
     1141 aacggcgaga gcctgctggc tgataatatg caggatggtg tccggattgc ccgtgataac
     1201 cccgacagcg gtgtggtggt gagaatcgcc ggtgaaggtc gtccgtggaa tcccggtgcc
     1261 atcaccggtg gtcgcgtgtg gggggatatc ccggacagca gcgtccagcc gggagccgga
     1321 aatggcgaac cggtcacggc agaggtgctg gcacagcggc aggctgaaga ggccatccgc
     1381 cgtgaaacgg aacgccgtgc agatgaaatt gtccgtaaaa tggcagagaa caaacctgac
     1441 ctgccggatg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga gcgtgaccgg
     1501 gctgacataa ctgaacggga agccgcgctg ccggagagtg tgctgcgtga atcacaacgg
     1561 gagcaggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg tctgcagcag
     1621 atagagcggg atatggttcg tgacctgcag aaagagaaaa ccctgggcgg agactgatac
     1681 aggaagataa ac
//
LOCUS       DAZFRE010000272         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFRE010000272 DAZFRE010000000
VERSION     DAZFRE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.278"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            4..301
                     /locus_tag="ACHKUI_005222"
                     /pseudo
     CDS             4..301
                     /locus_tag="ACHKUI_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(619..744)
                     /locus_tag="ACHKUI_005223"
     CDS             complement(619..744)
                     /locus_tag="ACHKUI_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO6601202.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(686..835)
                     /locus_tag="ACHKUI_005224"
                     /pseudo
     CDS             complement(686..835)
                     /locus_tag="ACHKUI_005224"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(1009..1676)
                     /locus_tag="ACHKUI_005225"
                     /pseudo
     CDS             complement(1009..1676)
                     /locus_tag="ACHKUI_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
BASE COUNT          404 a          501 c          458 g          313 t
ORIGIN      
        1 tgaatggcac cggacacaac aacgggcagg gacccgccgc aacgacgtga agcacggttt
       61 gcgaaacggc gttgcgcctt acatcccggc ggccttttgc cgggatgcca ggagcgcagg
      121 ccacggcgcg acagaggcgg cgcgaccgga acggtcgctg caagcccggg gtttcgcgtc
      181 agggatggaa gcccgccagg gcggagacag cttcgctggc tccgttcacg acagcccgcc
      241 ccgccagggg gacgcccgga aaaggcataa gcaggagaac aacaaaacag acaataatca
      301 gggcagcagt aatcagagtc agagcagtaa tcagagtcag agcaggagga aaaagagaat
      361 aatcaaaggg aaggtgatgt gatttctggc aagtcataaa cagagaaggc gggggaaaag
      421 aacgggcgcc gcaggcgcac gttcatcccc gttcccggag ggaaacggcc ttcctggcag
      481 tctggttgtt cagcccgtac aatgccagca aggagaaagg gaccaccggc gaaccagcag
      541 cccctttata aaggcgcttc agtgcagtca gaccagcatc agccctgaaa aggcgggcct
      601 gcgcccgcct ccaggttgct acttaccgga ttcgtaagcc atgaaagccg ccacctccct
      661 gtatccgtct ctgtaacgaa tctcgcacag cgattttcgt gtcagataag tgaatatcaa
      721 cagtgtgaga cacacgatca acacacacca gacaagagag ctgcgtggta gtttcatggc
      781 cttcttctcc ttgcgcaaag cgcggtaaga ggctatcctg atgttgtcta agcatgcagg
      841 ggcctcgtgg gttaatgaaa aattaactac ggggcttttg tccttctgcc acacaacacg
      901 gtaacaaacc accttcacgt catgaggcag aaaacctcaa gcgccgggca catcatagcc
      961 catataccag cacgctgacc acattcactt ttcctgaaaa taactcgctc attcaggctg
     1021 tttacgggca atccgttgtg attgttgccg catcacgcca cctcccggag tttgttcggc
     1081 accatccacc gctccatctc acggacagca tgaccggcct cctttgctgc cagactggct
     1141 tccgcgataa cctgccagaa tggcatatcc tgccactgcc agcgttccat tccggccagt
     1201 gacggaaaac gctcgcccca ctgccagaac agggtttcca ggtcatgctc tgccacccga
     1261 tcactccagc gtgcatgcca gcttccgact gtttccggtg aatggaacgc cagttcgatt
     1321 tcagcctggg cgaccagcgc ctggtacgca cgggctttct gctctgcctc cctcgcctgc
     1381 tggcggctgt acttctgcat gaccagcgcg gatttatggc gactgcgttc cctgacacaa
     1441 aaccgcaccg acgggaacaa gcgttctgct gcatcaccgg gaaggggaag gaggcagacc
     1501 tcgccctgtg tttcaatcag cacatcgatg gcatacaacc actgaagacg gtttccgtgc
     1561 agttcttctg cggtcaggga tggcatgaaa aatcgcaggt cagacagcga tatcctgcgg
     1621 cttccgttca gacagcggaa aaacgccccc gcatacggat attccgccag cgtgtt
//
LOCUS       DAZFRE010000273         1671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFRE010000273 DAZFRE010000000
VERSION     DAZFRE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.279"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            4..738
                     /gene="nanR"
                     /locus_tag="ACHKUI_005226"
     CDS             4..738
                     /gene="nanR"
                     /locus_tag="ACHKUI_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6601203.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(774..1670)
                     /locus_tag="ACHKUI_005227"
     CDS             complement(774..1670)
                     /locus_tag="ACHKUI_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6601204.1"
                     /translation="MMLSLFNKSTIVDLSSETISESKSLSGRIYQDPEHNGEFELDRV
                     LTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPEFLSQGYTLFVLSY
                     RLPSPVEGENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHIASTFVNQI
                     CDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTSENIIQYSA
                     DLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSIQWA
                     TDLSVGGWIKLFMDWMYSHGFK"
BASE COUNT          579 a          347 c          291 g          454 t
ORIGIN      
        1 tatatgtccg acaacaaaaa aaccgtgtta cccgggctaa caacgaccag aaagcccatt
       61 gttacaaaaa aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag
      121 gagataaggg aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt
      181 ggaagaccat ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat
      241 aacaagggag aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta
      301 tcaggcattg ttaaagattt tttatctaca gctgatggca tcaaggattt tgaaagacta
      361 cgcatctttt ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat
      421 attcacaggc ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg
      481 tatgctcaat atgatattga atttcatagt atacttgctg atatacctga caataaagta
      541 ttaaaaacaa tacactcagc attttctgaa tggctgataa tggccaaacc tctggaaaag
      601 aataaaatga catatgaaaa taacaaaaaa acccatcagc agcatattga aatacttacg
      661 gccataaaag agcatgattg tgataaagct gaacagctaa tgtataatca cctttataaa
      721 cgaattgaag catcatagtt actcgacgct tcaccaggta gtcaccataa atattattta
      781 aatccatgag aatacatcca gtccataaac agttttatcc atcctccaac agaaagatct
      841 gtggcccact gaatactaaa accatgccct cctttctcat agagatgcat ttcagcagaa
      901 acgcctttct ctattaggcc acggtacatg agcagactgt tttcaattga cacaagatca
      961 tcatcacaac aatggagtaa aaacacagga ggaaaacatg atgtgacatt aagatccgca
     1021 ctatattgta ttatattttc tgaggtacta ttgtccccca gtaattgctt cctggagcct
     1081 ggatgtgtca catcatcttt cattgatatt acaggataaa ttaacccgac aaaatcggga
     1141 atagcgaaaa tctcttgttc tttactgtca gaagaacgtt tgaaatcaca aatctgattc
     1201 acaaacgttg aggcgatatg ccccccagca gaaaatccta atacaccaat acgatgacgc
     1261 tgtagatgcc atttatcagc attcttacga atataaagca ttgcagatat tgcatcaata
     1321 aaagttttgt ttatattttc tccctcaact ggcgatggta atctgtaact aagaacaaat
     1381 aatgtatagc cctgagataa aaactctgga gcaagagctg taccttcctt atctaacata
     1441 acagcctggt agcccccccc gggaataatc aatattcctg ttccatttgg tttatctggt
     1501 aaaaaaataa ttaactgagg agcataaata tctgtcaata cacgatcaag ttcaaattcc
     1561 ccgttatgtt caggatcctg ataaatacgg cctgaaagag atttactctc agatattgtc
     1621 tcacttgaca agtcaacaat agttgattta ttaaacaagc ttaacatcat t
//
LOCUS       DAZFRE010000274         1665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFRE010000274 DAZFRE010000000
VERSION     DAZFRE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.280"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            21..560
                     /locus_tag="ACHKUI_005228"
     CDS             21..560
                     /locus_tag="ACHKUI_005228"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6601205.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            622..855
                     /locus_tag="ACHKUI_005229"
     CDS             622..855
                     /locus_tag="ACHKUI_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6601206.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAVQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            920..>1665
                     /locus_tag="ACHKUI_005230"
     CDS             920..>1665
                     /locus_tag="ACHKUI_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6601207.1"
                     /translation="MSVTESKVKTERKSSRKPAKTQETVLSALLAETAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIQGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVVGNTKFR"
BASE COUNT          393 a          441 c          521 g          310 t
ORIGIN      
        1 tcaacagaag gagatacatc atggcagttc gtggcattaa caaggtcatt cttgtcggtc
       61 gtctgggaaa agatccggaa gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc
      121 aggtggccac gtcggaaacc tggcgtgaca aacagacggg ggagatgcgg gagcagacag
      181 aatggcatcg cgtggtgctg ttcggcaagc tcgcggaagt ggctggcgaa tatctgcgca
      241 agggcgtgca ggtctacatc gaaggtcagc ttcgcacccg tagctgggaa gataacggta
      301 tcacccgtta cgtcaccgaa attcttgtta agaccacggg caccatgcag atgctgggac
      361 gtgccgcagg tgctcagact cagccggaag aggggcaaca gttcagcggt cagcctcagc
      421 cggaaccaca ggcggaggcc ggtacgaaaa aaggtggcgc aaaaacgaaa ggccgtggac
      481 gaaaggccgc gcagccggag cctcagccgc aaccgccgga gggtgacgat tacgggtttt
      541 cagacgatat cccgttctga acgactgact gtgacaacgg ccccgccccg tcctgtacgg
      601 ggcatcaccg gagagatgag gatgagcgaa tatttcagaa tacttcaggg gctgcctgac
      661 ggctccttta cccgcgaaca ggccgaagcc gttgccgtac agtaccggaa cgtctttatc
      721 gaggatgatc acggcgaaca gtttcgcctg gttgtccgta ataacggtgc aatggtctgg
      781 cgtacctgga attttgagga cggtgccggg tactggatga accatgtcat ccgtgatttc
      841 gggattctta agtaagagag gtgccggacg gggatcacgt ccggcagaca tacaaccacc
      901 ccggtcaaag gagaaatata tgtcagttac agagtctaag gtaaaaacgg agcgtaaatc
      961 cagccgtaaa cctgcaaaaa cgcaggaaac agtcctgtcg gccctgctgg cggagacggc
     1021 tgaagtgagc gtgccgctgg cctcgctgat taagtcaccg ctgaatgtgc gcacggtgcc
     1081 gtattctgcg gagtccgtca gcgaactggc agagtccatt cagggcgtcg gcctgctgca
     1141 gaatctggtt gttcatgccc tgccaggtga ccgttacggt gtcgccgcag gcggtcgccg
     1201 actggcagca ctcaacatgc tggcagagcg caacatcctt ccggctgact ggcctgtccg
     1261 cgtgaaggtt attccgcagg aactggcgac tgccgcatca atgactgaaa acggtcatcg
     1321 tcgggatatg caccctgccg aacagattgc cggattccgt gcaatggcgc aggaaggcaa
     1381 aacgcctgca caaatcggtg atttgctggg ctattcaccc cgccacgttc agcgaatgct
     1441 gaaactggca gaccttgcgc ctgtcatcct cgatgcgctg gcagaagacc gcatcaccac
     1501 agagcactgt caggcgctgg cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga
     1561 agctgcctgt cagtcgggat ggggcggaaa accggaagta cagaccattc gtcgtctggt
     1621 gaccgaaagt gaagtggcgg tggtggggaa cactaaattc cgctt
//
LOCUS       DAZFRE010000275         1663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFRE010000275 DAZFRE010000000
VERSION     DAZFRE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.281"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1040)
                     /gene="traH"
                     /locus_tag="ACHKUI_005231"
     CDS             complement(<1..1040)
                     /gene="traH"
                     /locus_tag="ACHKUI_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO6601208.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYL"
     gene            complement(1027..1419)
                     /gene="trbF"
                     /locus_tag="ACHKUI_005232"
     CDS             complement(1027..1419)
                     /gene="trbF"
                     /locus_tag="ACHKUI_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO6601209.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRENKLREGGNNASH"
     gene            complement(1400..>1663)
                     /gene="trbJ"
                     /locus_tag="ACHKUI_005233"
     CDS             complement(1400..>1663)
                     /gene="trbJ"
                     /locus_tag="ACHKUI_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO6601210.1"
                     /translation="LIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVVSLCLTFSV
                     SVVFFVGAIVGWISVSEIYYSRMNGLNESSEISEETYNERKKK"
BASE COUNT          444 a          428 c          359 g          432 t
ORIGIN      
        1 ataagatatt taaagacggg gacggtggtg ctggaaatga agcctttttc cctgtcatcc
       61 agtggagtat cactcaccgc tttattctga atactggtga gcagttttgt aatctgtgat
      121 ttcagggctt tatcccgggc gatggtcaca tttgcatccg ccaccacctt cagacacttg
      181 tccgaatcgt tgcagtgata aacttttgcc gttcccccct ccataatcgc ccggaggata
      241 tcgcggtcag tggtacgcgc cggcaggggg gtaatctcgc tgtcttcccc aaaaatcagc
      301 gtaccggtga gcgtcataat aaattctttc agttctttgt taccatcaag catcctgttt
      361 ttggacagcg agtcccagat gatattgatg ttcttcagca cccgttcctt atctttgtca
      421 ctggccttgt cacgaacaga cgatgactgt cctccgaccg tgcagccctg acgggacgcg
      481 gcccagtcag caaaaatatt gctctcaccg gcaatgtcct gacagacctt ctgctgtgac
      541 acctgagtct gaggaaataa tcctccgaca atcccctgcg ccacctgaca ggagctgagg
      601 ttcatactgt taatgtcgct tgccattttc tgcaggaagt cttttgcggt tttgatttcc
      661 ggcaccgttg cctgcagggc aaggtcaaaa aagtaaccgg cagcgttgct cataatctgc
      721 ttaacaaaac gctgcaactg atcgccatta ataaaactga acgagccgag ataggcgtcg
      781 atgccgccac atccggcatt gatatccggc agcgtcatgg aaatcagctg gatatttttg
      841 acctgggtgc gggcatacag ggagccacca taggcatacc cggcggcctg cccctgccag
      901 acgcccggtt gtgtggtatt ggaagcaaat cccagcttat taaagaactg attcatgtcg
      961 ctgttcacat ccgctgacgc tgcgggtgtg accgtcagta aagcggcaca aagaaccaga
     1021 agaggtttaa tgcgaagcat tattgccacc ctcccttaat ttgttctccc ggcgcatacg
     1081 gcgtttcagc cggaatattc tgatcccccc gtttattaat tcatttaaag ggttcctgac
     1141 gtatacgata agaaatccac ctgcaaacgc ccataacgta tacggaatga catacatcag
     1201 gatgttccac atataacgaa aattatttaa ttccgggtca gctttccacg aatcaataca
     1261 ggcatcaaaa atactatgca taatcaccca taaaaaagtt ccggcagcaa cgaacacaaa
     1321 aaacagatca aaatatatat cagaaaccct gcgttcataa tcataatccc gtttagtttt
     1381 tctgtttttt agttccgcat catttctttt ttctttcatt ataagtttcc tctgatattt
     1441 ccgatgattc attcagaccg ttcatgcggc tgtaatatat ttcagacacc gaaatccatc
     1501 ccacaattgc ccccacaaaa aacacaacag aaacagaaaa ggtaagacac agcgaaacga
     1561 caatttccac agggctggcg ggataaaaaa gcccctgacg gataaagggg atattcaggc
     1621 tgacgattat tcccgtcgcg atgccggata ttccggcaat gag
//
LOCUS       DAZFRE010000276         1654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFRE010000276 DAZFRE010000000
VERSION     DAZFRE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.282"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..477
                     /locus_tag="ACHKUI_005234"
     CDS             <1..477
                     /locus_tag="ACHKUI_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFO6601211.1"
                     /translation="SEKESKLTQAISEKEQILKQLHIVQEQLEHYFIENQEIKKKLPP
                     VLYGAAEQIKQELGYRLGYIIVSYSKSLKGIITMPFALIRECVFEKKRKKSYGVDVPL
                     YLYADADKAERVKKHLSYQLGQAIISSANSIFGFITLPFKLIVVVYKYRRAKIKGC"
     gene            complement(574..1608)
                     /locus_tag="ACHKUI_005235"
                     /pseudo
     CDS             complement(574..1608)
                     /locus_tag="ACHKUI_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323773.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          454 a          308 c          350 g          542 t
ORIGIN      
        1 agcgaaaaag aatcaaaact cactcaggca atatcagaaa aagaacagat tttaaaacaa
       61 ttgcatatag tgcaagagca gttggaacac tattttatag aaaatcagga aattaaaaag
      121 aaacttccac ctgtgctata tggagcagct gagcagataa aacaagagtt aggttatcga
      181 cttggttata ttatagtctc gtattctaaa tccctcaagg ggattattac catgccattt
      241 gcacttatcc gtgagtgtgt ttttgaaaaa aaacgtaaga agagttatgg cgttgatgtg
      301 ccactctatt tatatgctga tgctgataag gctgaaagag ttaagaaaca tttatcttat
      361 caattagggc aggctattat ctccagtgct aattcgatat ttggattcat tacccttcca
      421 tttaagttaa ttgttgttgt ttataaatat aggagagcta aaatcaaggg ctgttaaaaa
      481 tgtgaaccct aatgagatat attgcaaatt ttatttttct ctttgtggtg ttttgctttc
      541 gttaaaatag ttagttattt tatttatata atatcacgca ttataatacc aatttatact
      601 tttgcaagtg accgtataga ttcgccacat attgcaaatt ttgttctctc gtaaaatatt
      661 ttcttctggt gtcagtaatt cgagcacttc atgctgtctt ttactgcagt atagtactag
      721 gttttcagct agtttctgat taaatatgtt tagctcttta aagagtctat ttatttaaat
      781 tcaatgaact gttctttcgg cgttcgctta aaacgttcag aggtgcgttc taggcgtaaa
      841 ggggtaggtc catagggctt gctagcggat tcctgcggtg ctttgtcgaa gtttcctggg
      901 aactctttcc cgttatctgc gatgccctgg ttgatatcga tagggaatag ccggtctgca
      961 cggcaaaaga agagtttgat gatatcacag ttaagtgacg gcacggcctg tgccagagcg
     1021 tagttgctgt gttcgtcgat catggtaatg acataacagc gcagctcgcc cattctgagc
     1081 tcaatagcat ctataccaac gagctcactg ctctttaccg ggcgatagcg ttttgatctg
     1141 cgggttggag gattgtattt tttatgaaca gtgtttttcc ttggggaggc agtcacatcg
     1201 gtatcagtcg cattttatcg tgtgcggccg tgaacatcct gccgatggtt gaaatgctcg
     1261 gacagaccag gcggtgctgt tcactccacg gcttcaagca aacaaaaatc tgttcttcta
     1321 ggttgggaag ctctattctc agtcgacgta tttcttacag aattacgaga tgccattgct
     1381 ttgtgcgatg tactagcgga gctttgctac gcgaaatagg tgcctctggg ccataataca
     1441 gcgttcgtgt ggatacagca aaaacttccg caactgtatt gatctcatgt ttctcccaga
     1501 agtatatttt ttcatcctta attttgtaat ctcaggtata acaaagtgtt tcatcacata
     1561 gatgttggca tggtaatgcc tcaaatatcc gccgcagata cgttgcatca acttagcatt
     1621 tccctcgctt gtccggagat aattgcaata tctc
//
LOCUS       DAZFRE010000277         1654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFRE010000277 DAZFRE010000000
VERSION     DAZFRE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.283"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..140)
                     /locus_tag="ACHKUI_005236"
     CDS             complement(<1..140)
                     /locus_tag="ACHKUI_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6601212.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDT"
     gene            complement(293..859)
                     /gene="slp"
                     /locus_tag="ACHKUI_005237"
     CDS             complement(293..859)
                     /gene="slp"
                     /locus_tag="ACHKUI_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6601213.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(1049..1426)
                     /locus_tag="ACHKUI_005238"
                     /pseudo
     CDS             complement(1049..1426)
                     /locus_tag="ACHKUI_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          459 a          307 c          329 g          559 t
ORIGIN      
        1 gtatcaataa tgacgaaggc attctgatgc aacattacgt cgatctcttc ttcgttctgt
       61 atatgaacga cattaccttt actcagaatg tttttcatcg cggtgaaaaa catcgtatcc
      121 ctggtaatta taagaaacat aaccgactcc tggtacggac attaacttta taatcagttt
      181 gccaaaggta attaacagga cagggcttat aataatagtt agcttactgt gtattaattt
      241 gatgtaatgc atgatgcaga tgctgagaaa gcatctgcat ctttcggtgg tgttatttga
      301 ccagctcagg tgttacctga ctcaccgcat tggtgtagta aggagccccc cagcccggtt
      361 ccggccagaa tgcaccatag ccgtaatccc acaggttata ggtggtatta accacttctc
      421 tcaaatgcca cacctggatg ccctgcatat tcacttccag gaagttatac gggactttat
      481 tgataaagcc aggttgttca ccctgaatgg taccgaggat ggtaacaaag tgattacgat
      541 agttcactgg atcaaggaag ccgctttgtc tggcgagcag tcggccctga tagttggctt
      601 caatatcagg cttcgcatag ctatccaacg gtaatacagc gatttctaac aacgtatccg
      661 ttttgccatt gataacgttg ataaccttcc cgccaaagcg cgcttgttga ccaacatata
      721 accccggctg gttatgaaca gcaacaaaac ttttttgaat atcaggttgg ttattgcctt
      781 tgatattttg cggaattgaa ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg
      841 caccttttgt catgttcatg ttactatcct tatcaacaaa tattaaaacc ataatagata
      901 tttctttata gttttcatct gattctgagg cgatatgcct gatagtaacg taaacctgtc
      961 tgatgtttat atctcttctg tttagttgag ctcagttaat ttttgggcgt ggcatgagta
     1021 tccattgttc cataatctat attcagaggt ttttaatctt ttcgtgtcta atgttattta
     1081 tctgattaag tattgattgt gtcttttgcg gaagtggtgt cgagcttatc gtttcaatgt
     1141 tattaataag acttacaagt tgagcctcaa ctttgagtag tctgcatttg tatatgtggt
     1201 gttcgtatgg cacgcattaa tttgttggcg ttgttgcgct aaatcaaatt gttcagaggt
     1261 gtatccattc tccagagaat ggggaattaa tggggtgttg ttcttcccgg ttatcacaag
     1321 gccacttcgg tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat
     1381 caccgttata tgtatttatt acacctgggg taaagtcaat tgacataaca ttccttattc
     1441 atcatttgtt ttatgctaag atttttaaat tggataatta gaacaggaat ggattttctt
     1501 ctacatacag taaatataat gcgtcatatc ttcctgatat aattaccttt gacttctctt
     1561 ttatttccat ctggtaatat ttgagtgcaa aaaaaggcaa taatcatatc gcaaaagaaa
     1621 acgttaacaa ctatcgaaaa tgctcgtttt aaaa
//
LOCUS       DAZFRE010000278         1617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFRE010000278 DAZFRE010000000
VERSION     DAZFRE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.284"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..827)
                     /locus_tag="ACHKUI_005239"
     CDS             complement(<1..827)
                     /locus_tag="ACHKUI_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6601214.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     "
BASE COUNT          391 a          369 c          432 g          425 t
ORIGIN      
        1 gtgtcacgga cggcacgcag aaccgtgcgt gcaacagagg caacacagac gctctccgct
       61 gtgtcaaata tccggtccac cgcaccggta aactgctcac gggcctgagc gcggacagat
      121 tccgtgcgca gctcgtcctg caggcgggtc atcaggggac tggcggcatg gtcgggaagc
      181 gctgtcatga gcgcactgac cagcgtatcc acttcccagc cggtgcgggc cgatacggcc
      241 acaaccggat gtacaggccg gaacagacgg aataccgcct ccgttttttc gcgaatgttc
      301 tgtgcctgtg cgggagaggg ctgaatgccg gccatatccc attcatggca gggctccgtt
      361 ttgtcggcct gtgtcaccac aaacagcacc tgctgatgtc cgcactgcag gatgtgtcgc
      421 cagaaatact catccacaga cagggcccgg tcatcggctt taatcagcca cagcaccagg
      481 tccagttcag gcagaatgtc acggtacagg gcttcatact ctgcatccct gtcccggctc
      541 tcgcccaccc cgggcaggtc agtgataacc atgctgtaac cgtgaccact cagacggaag
      601 cgctgcactt cccgggtgcc ggcgtgaaca tcactgaccg gtgtgacctc cccctgaaac
      661 agggcattac agagtgaaga tttaccggcc ccgcttttac ccataatgcc aatcacgggc
      721 tcgtgacggg tgagtttgcg cagatgttcc aggatgtgac gggaaagcga gtaaggcagg
      781 gaggagagcg gtttttcaat tgcctcaatg gcatcagacg gattcataca tgtattcccg
      841 gtgaaaaaag acaaaacccc gcacaccgga aaggtggcgg gggatccacg gagataatat
      901 ctgtctgaaa ttgtttggaa tcgatgggga tgttgcgtat taacatgaag gagggcagga
      961 gttatccgtt attgggtgat atccttccat gaaaatacgc ggttattgtt aaacagcagg
     1021 ctgattactc cgtaaaggag aaaaatattc atcttgcagc ctgattatca ggtgtgttta
     1081 tgtttactga ggtgagtatt tttcagtata ttgtcggggc agtcagtggg agatattaaa
     1141 tttacgttta gagttatctt gcttctgctg tgtattttta taagagtgcg cacgtaatga
     1201 tatctgttga taccatgttg ttaagtgttt cccttccggg tcatgcgtac ttgcaaaatg
     1261 gcatctgaat cgatcacggt ttggccaaac cgtgatcgaa tagataggaa tacgcacgta
     1321 atgattaaat gcttttgtta caccattctt ccggcatgaa tatttatcca gttccctttt
     1381 ttatttgtgt agtgatactg tcggaatagc gttcagtaat gtgaagtgtc ctgtaaaaaa
     1441 actttaataa atgtgtcatc ctggtcagga aatatctgct gagatattat tagttggtgg
     1501 tgtttttact gagtaatagc tggcttttaa ttcctgaact gaaatactgt cgctcgtcag
     1561 ggtttaatta aaactggatg atgatgtgaa aatgaaggct ggtttttgtg tttgtcg
//
LOCUS       DAZFRE010000279         1541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFRE010000279 DAZFRE010000000
VERSION     DAZFRE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.285"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(153..1523)
                     /gene="cpsG"
                     /locus_tag="ACHKUI_005240"
                     /gene_synonym="manB"
     CDS             complement(153..1523)
                     /gene="cpsG"
                     /locus_tag="ACHKUI_005240"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6601215.1"
                     /translation="MSSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINRKLAHPVEAINR
                     VEQHFSREVLAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVQVMVIHTQEI
                     LSILTS"
BASE COUNT          370 a          420 c          365 g          386 t
ORIGIN      
        1 taaagttgtg taatgctttt ttcgttctca ttattatttt ccagtcaaca ttaattcaac
       61 cacattaaat aacatattaa aaatcttacc acaaatcccc aattcaaaaa aaatatatat
      121 taattaagcc ctaacttgtc agggcttatt ctttatgacg tcaaaattga taatatttct
      181 tgagtatgga ttaccataac ctgaacatca ccgcgagatt ccacattcag gcgcaccacc
      241 ggttcggtgt tggaagagcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca
      301 tcggtgcgat ccaccgccag cacctcacgg ctaaaatgct gttccacgcg gttaatcgcc
      361 tcaacagggt gcgccagttt tctgttgatc tcaccgcttg ccggaaacgc cgccatccgg
      421 tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc
      481 cacgggatca tgccgctgtc acagtaagcg aaatcgcgga agtaatggtg agcgctcatt
      541 tcgccaccgt agatggcgtc ttccttgcgc atacgttctt taataaaggc gtgtcctgtt
      601 ttcgacatca ccggcgtgcc gcccgcggcc gtcaccacat caatggtgtt ccaggagaga
      661 cgtggatcgt ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg
      721 ccgacaatgt agtagccctc aataaactgc cctttttcgt caaacaggaa acagcggtca
      781 aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg
      841 tcgtcgcgac attccggcag caacgggtta ggaataccgt tggggaaatt gccgtccggc
      901 gtgttatgca ctttgattaa ctccaccggt gcgccgaggg ctttaaagcg ggcttcgata
      961 gcgtccacca ccggacccgc tgcgccgttc ccggagttga tcaccagctt gagcggcgta
     1021 aggtttttga cattgatata accgaacagg tgatcaacgt aagcgtcacg cagattgatt
     1081 tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa agtcgttagc ttctgccaga
     1141 cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc gcgcaccagc
     1201 ttcatgccgt tgtaatccat cggattatgg ctggcggtaa cttcaatgcc gccatccacg
     1261 ccgagatgga acgtggcgaa ataaatctct tcggtgccgg acatgccaat atccagcaca
     1321 tcgacgcccg catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga
     1381 cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca
     1441 atgcgccagg cgatatcttc attcagttct tcacctaatt tcccgcgaat gtcgtaagct
     1501 ttaaaacaag ttaaagaact catattttcc aacccatact a
//
LOCUS       DAZFRE010000280         1540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFRE010000280 DAZFRE010000000
VERSION     DAZFRE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.286"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            5..667
                     /locus_tag="ACHKUI_005241"
     CDS             5..667
                     /locus_tag="ACHKUI_005241"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6601216.1"
                     /translation="MDYMLNSTTSSGKRTGILAIGAHPDDIELGCGASLSYYSNEKVY
                     IISVVMTPGCCGAEYIDRHKESQEALNMLGCNKLIPLNFEDTRLYLQIKEMISSLEYI
                     IENETPQDVEITRVYTMNNTDRHQDHIAVYQASMVACRYIPQILGYETPSTCLSFLPQ
                     VFESVDEVHFNKKILALQKHKSQIHRRYIQPEYIIAQAKFRGQQVSHSMCEGFVVHKM
                     VL"
     gene            681..>1540
                     /locus_tag="ACHKUI_005242"
     CDS             681..>1540
                     /locus_tag="ACHKUI_005242"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601217.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVG"
BASE COUNT          438 a          307 c          358 g          437 t
ORIGIN      
        1 aaggatagat tacatgttga attctacaac atcttcggga aaaagaaccg gcattttagc
       61 aattggtgcc catcctgatg atatagaatt aggatgtggt gcatctcttt catattattc
      121 taatgaaaaa gtatatatta tatctgttgt catgacacct ggttgctgtg gcgcagagta
      181 tattgatcgt cacaaggaat cacaagaagc attaaatatg ttaggatgca ataaactaat
      241 ccctctaaat tttgaagata ccagattata tttgcaaata aaagagatga tatcatctct
      301 ggaatacata attgagaatg aaacgcccca ggatgtggaa attactcgag tttacaccat
      361 gaataatact gacaggcatc aggatcacat tgctgtttat caggcatcaa tggttgcctg
      421 tcgttatatt cctcaaatac taggatacga aacaccaagt acatgtctgt catttttacc
      481 tcaagttttt gaatctgtag atgaggtcca ttttaataaa aaaatattag cattacaaaa
      541 gcataaaagc cagatccatc gaagatacat acagccagaa tacataattg ctcaagccaa
      601 attccgagga caacaggtta gtcattctat gtgtgaaggt tttgttgtcc ataagatggt
      661 gttgtaacgg agtaatagaa atgaaaactt tgttatttat cttcatgacc atagcaatgt
      721 tgctatggtt tttaagtacg ctaagacgta aaccctgtca aaagaaaggc tgtattgacg
      781 ccattatacc agcttataac gaaggcccgt gtctggcgca gtcactggat aatctgctgc
      841 gtaaccctta tttttgccgg gtaatttgcg ttaacgacgg atccacggac aataccgaag
      901 cggtcatggc ggaagtcaaa cgcaaatggg gcgaccgctt tgttgccgtc acgcaaaaaa
      961 acaccggtaa aggtggcgca ctaatgcatg gactaaaata cgccacgtgt gatcaagtat
     1021 ttttaagtga tgcagatact ttcgttccac ctgataatga tggaatgggc tatatgctgg
     1081 cagaaattga gcgcggcgct gatgccgttg gcggaattcc ctccaccgcg ttaaaagggg
     1141 caggtctgct gccacatatc cgcgccaccg taaagttgcc gatgattgtt atgaagcgca
     1201 cgctacagca gtttctgggc ggtgcaccgt ttattatcag cggtgcctgc gggatgttcc
     1261 gtactgatgt attgcgtaag ttcggtttct ctgatcgtac taaagtcgaa gaccttgatc
     1321 tcacctggac gttggtggca aacggctacc gtattcggca ggcgaatcgc tgcatcgtat
     1381 acccacagga atgtaacagt ccgcgtgagg agtggcgccg ctggcggcgt tggattgtgg
     1441 gctacgcggt ctgtatgcgc ctgcataaaa gacttttatt tagccgcttc ggtatcttca
     1501 gtatatttcc tatgctgttg gttgtgcttt atggcgttgg
//
LOCUS       DAZFRE010000281         1534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFRE010000281 DAZFRE010000000
VERSION     DAZFRE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.287"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1534
                     /locus_tag="ACHKUI_005243"
     CDS             <1..>1534
                     /locus_tag="ACHKUI_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6601218.1"
                     /translation="TVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHA
                     IGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARTNQQHSENST
                     GKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDRED
                     WKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEG
                     RNQSATDTLSSPVPETDAVETAMPDNEKTECEMEDEPSVEREGPFYFLFTDKDGEKYG
                     RANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTIAQPEPVKVTADEV
                     NKIMQAANISQPDANKLLAVSRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNG
                     QKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQET
                     FDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITS
                     TS"
BASE COUNT          500 a          363 c          396 g          275 t
ORIGIN      
        1 tcaccgttga tatggttatt agcgcacagg aattgttgca ggaagacatg gcaacattcg
       61 acggacatat cgttgaagcg ttgatgaaaa tgccagaagt taacgccatg tatccggagc
      121 ttaagctgca tgccatcggg tgggttaagc ataaatgtaa gcctggtgcc aaatggcccg
      181 aaattcaggc agagatgcgc atctggaaaa aacgtcgcga aggtgaacgc aaggaaaccg
      241 gaaaatacac gtctgttgtt gatctcgccc gcgccagaac caatcaacag cacagtgaaa
      301 attcaacagg aaaaatcagc ccggtcattg ctgccattca tcgcgaatac aagcagacat
      361 ggaaaacact ggatgacgaa ctggcctacg ctctctggcc tggtgatgtg gatgccggaa
      421 acattgacgg cagcatccat cgctgggcaa aaaatgaagt tatcgacaac gaccgcgaag
      481 actggaagcg tatctcggca tcaatgcgca aacagcctga tgcccttcgc tacgaccgcc
      541 agactatttt tggccttgtc cgtgaacgtc cgatcgacat tcacaaagac cctgtggcac
      601 tgaacaaata cattactgaa tacctgacta caaagggcgt gtttgaagat gaaggaagaa
      661 atcagagcgc aactgatact ctctcgtcgc cagtaccaga aactgatgca gtggaaacgg
      721 caatgccgga caacgaaaaa accgaatgcg aaatggaaga cgaaccatct gtagagcgtg
      781 agggaccgtt ctacttcctt ttcaccgata aggacggcga aaaatacggt cgcgcaaaca
      841 aactttctgg tctggaaaaa gcactggccc tgggagctac ggaaatcaca aaagaggaat
      901 acttcgcacg taaaaacggc acgtactctg gttcacaaca aaatactggt gcatctgaca
      961 cgatcgcaca accagagccg gtaaaagtta ccgctgacga agtaaacaaa attatgcagg
     1021 cagccaatat cagccagcct gacgccaata agttgcttgc tgtatcacgt ggtgaatttg
     1081 ttgcagggat tagcgacccg aatgatccga aatgggtgaa ggggattgaa acccgcgatt
     1141 cagtgaatca gaaccagcaa gaaacggaac agaacggcca gaaagcggaa caaaacagcc
     1201 caaatgcgtt acaaaacgag ccagaaacga aacagcctga accagtggcg caacaggaag
     1261 tggaaaaagt ctgcaccgcc tgcggtcaga ccggcggcgg caactgccct gattgtggcg
     1321 cggtgatggg cgacgcaaca taccaggaaa cattcgatga agagtatcag gttgaagttc
     1381 aggaagatga tccggaggaa atggaaggcg ctgaacatcc acacaaggag aacactgacg
     1441 gcaatcagca tcacgatagc gataatgaaa ctggcgagac ggcagatcac tcaattaagg
     1501 tgaacggtca tcaagaaatc acatccacca gcag
//
LOCUS       DAZFRE010000282         1527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFRE010000282 DAZFRE010000000
VERSION     DAZFRE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.288"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..426)
                     /locus_tag="ACHKUI_005244"
     CDS             complement(<1..426)
                     /locus_tag="ACHKUI_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6601219.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKRLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTELKFFTQSVK"
     gene            complement(809..1471)
                     /locus_tag="ACHKUI_005245"
     CDS             complement(809..1471)
                     /locus_tag="ACHKUI_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFO6601220.1"
                     /translation="MSIEHILIGKHHGGSDDWRTPYRLFRNLHREFGFSMDGAATKHD
                     ALLPRFTDDIHNQSWVGEKVFCNPPYSDIPSFLLKASESDLAVFLIPYRPHTTYWLKH
                     IFTNPLCHEIRILHRAVKYLPPAGHNGLTTRSPFAAAVVVFKKESRRHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LG"
BASE COUNT          455 a          301 c          312 g          459 t
ORIGIN      
        1 tttgactgac tgagtaaaaa attttaactc agtgggtaaa gaattttgac tcagtgggtc
       61 atctttcttg actgactgag taactttaga ctccagcttt gcctttctgg cttccttagc
      121 tgtaagctct gttaaaactt ccggtacttc atcaagtgaa taaactttaa taacacactg
      181 attgcttgca acgttagttt tcttgattaa accatgtgcc tcaagtcgtt tcatacatgc
      241 aatcacggat gttcttgtga ttccagcata ctgaccaaga tgagcatatg aaggggtata
      301 atattgcccc ttatcgtgat aaaacttttg ttcattcaac atgacagtgt aaataatacg
      361 gtcggcagct ttaacgccat tagtcatctg ataaatacgg tcatcaaaca tgtgaaattt
      421 ttccattata ttttcctttt taaaaataaa acaaagaaag ggctgaccac gtatagtgac
      481 caaccccaga gggagtgaat taattagaag atggactgat aaaggaaccc agaaatctaa
      541 gtgattacgc gagtattaac agcaaaagga aaagccttga ttgaataaga ggccgtgaaa
      601 atccgtcaat accctgacgc ctcccaacgt tgtaacctct taatgttctg agttctttta
      661 tcagcccact atgaaactat accatgtgtg attataaatt gcaacaatta tttcacagaa
      721 taattactga ttggtcattt attgttcaca ggaatacttc ttaatatctg aatcgcctgt
      781 cttgtaaatg gccatattta tgagaatttt agcccaacct ttgagcaatc ctgtatgacg
      841 tgctcagagg cattctgaga gcatctgcaa tgcacttaat gggttttcct tgcctgtata
      901 gctttatgaa gctgtcgagc gtctcaggtg ggtaaatggt gggtcttcct cttagtccac
      961 cacggttaat gatggttaac ggtaatagcg tgtcagcaca gcaaaccatc tgcgtaattt
     1021 catgcctgcg tgattctttt ttaaaaacaa cgactgcagc agcaaacggt gacctagtag
     1081 taagcccgtt atgccctgct ggtggaaggt atttaactgc gcgatgaaga atacggattt
     1141 catggcaaag cgggtttgtg aagatgtgct taagccagta tgtggtgtgg ggtcggtacg
     1201 gtatcaggaa taccgctaaa tctgattcag acgcttttaa gaggaacgac ggaatatcgc
     1261 tgtatggtgg gttacagaat accttttcac caacccatga ctgattgtgg atatcatcgg
     1321 tgaaccgtgg tagcaatgcg tcatgcttag ttgcagcacc atccatcgaa aaaccaaatt
     1381 cacgatgcag gttacgaaat aaacgatacg gtgtacgcca gtcatcagaa ccaccatgat
     1441 gcttcccgat taaaatatgc tcaattgaca taagaaataa aattttttat tgcaatcact
     1501 cttgacaagg tttactctct tttaaga
//
LOCUS       DAZFRE010000283         1505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFRE010000283 DAZFRE010000000
VERSION     DAZFRE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.289"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(103..363)
                     /gene="repA"
                     /locus_tag="ACHKUI_005246"
     CDS             complement(103..363)
                     /gene="repA"
                     /locus_tag="ACHKUI_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO6601221.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLTQLCKDSGLTQKEMIEHWILKEKAAMTDANRK"
     gene            complement(648..797)
                     /locus_tag="ACHKUI_005247"
     CDS             complement(648..797)
                     /locus_tag="ACHKUI_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6601222.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(996..1433)
                     /locus_tag="ACHKUI_005248"
     CDS             complement(996..1433)
                     /locus_tag="ACHKUI_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601223.1"
                     /translation="MKYDDVSELMIKCEHNILIVGNTYSRVDNFFKGYSVPDSKYYDF
                     TQINSDSDAERNDHAVSFIRDAMNSEEHIIIFNCVGWPDLGGGSAVSQFAMMARKFGK
                     QLIVAVSEKDAIKLKDNFNIIGTLSYGKENFIAMSHTKSEFCS"
BASE COUNT          404 a          372 c          279 g          450 t
ORIGIN      
        1 agaaccagtt ccctgccacc ttacggcgtg gccagccaca aaattcctta aacgatcagt
       61 aatctagcat ccgcaattca aatgttcaag aaggtaactt agttactttc tattcgcatc
      121 agtcatcgca gctttttctt tcaaaatcca gtgctctatc atttcttttt gagttaagcc
      181 cgaatcctta caaagctgtg tcaaatcatc tttgagttca ctctggatga aaacattaat
      241 cgctttatgt gtcccctttt tccgggaaac agatatgcgt tgtttctcgg ctccggttaa
      301 cggattacct tttctgtaaa tgcgtttcgg tgaggaagtt actgcatttt cgatctgcga
      361 catctcgcct cctcaatact caaacaggga tcgtttcgca gaggatacta cagttttttg
      421 aaatcagcga cttgagaatt gtgacgaaga tctgtgttgg cgcacaaatc atgtctgatt
      481 actgttgttt aatgtgattt ttgttgcgaa atggctgaaa aaacaggaaa tgccttaacc
      541 tgtgtgccgg ccccgggcgg gaccggcggt ttctggcatg tcgggtgtct gcccctgaaa
      601 taccccacac ttccggaaag cggggacttc cgcccccgcc cggcagctta cttccgtgct
      661 tcgtaggcca gagttacctg caccactgta tttcccctgt gaatattcag ctcacataac
      721 cgttccctga atatcagtga aaaacacaac accgtggcgc acacggcgag caacccgata
      781 agggtatatt tcgtcataaa ctttgacctc tctgcaagga agaggctaca atcagtagtg
      841 ctacgtactt catgcaggcc tcacgagtta atggattaac aagtggggtc ttcgcatttc
      901 tggtacctgc gtcttgcctg aaccagaaag gctcagacac ccgccgcgat aataccacat
      961 tccctgttgc tgaccattac ctccatttcc cgcttttaac tacagaactc gcttttcgta
     1021 tggctcatgg caataaaatt ttctttacca tatgacaacg ttccaataat attaaagttg
     1081 tcttttaatt ttatggcatc cttttcactt acagcgacaa taagttgttt accaaatttt
     1141 ctggccatca ttgcaaactg cgatactgct gatccaccac ctaaatcagg ccacccaaca
     1201 caattaaaaa taattatatg ctcttcacta ttcattgcat ccctgataaa ggagacggca
     1261 tgatcatttc tctctgcatc tgaatcactg ttaatctgag tgaaatcata atatttagaa
     1321 tctggtacag aatacccctt aaaaaaatta tcaactcggg agtaagtatt accaacaata
     1381 agaatgttgt gttcacattt aatcatcaat tctgacacat catcatattt catatattca
     1441 ccattatgag ataaaatctg gatacaggtg gttccggctt tccccctgtg ccaccatttc
     1501 ccggt
//
LOCUS       DAZFRE010000284         1468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFRE010000284 DAZFRE010000000
VERSION     DAZFRE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.290"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..723
                     /locus_tag="ACHKUI_005249"
     CDS             <1..723
                     /locus_tag="ACHKUI_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="similar to VirB4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6601224.1"
                     /translation="GQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIE
                     NRMYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDF
                     DSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQW
                     FSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKK
                     SGPEMASLEAWLEEHEKYRSVA"
     gene            720..1106
                     /gene="trbI"
                     /locus_tag="ACHKUI_005250"
     CDS             720..1106
                     /gene="trbI"
                     /locus_tag="ACHKUI_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO6601225.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1103..>1468
                     /locus_tag="ACHKUI_005251"
     CDS             1103..>1468
                     /locus_tag="ACHKUI_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6601226.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQ"
BASE COUNT          335 a          380 c          450 g          303 t
ORIGIN      
        1 ggccagtatt ttaactccga tgagccgtcc ctgcgggatg atgcgaaaat ggtggtgctg
       61 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt ctccctgatt
      121 atctacatcg agaacaggat gtaccgcacg ccgcgtactc tcaagaaact gaacgtcatt
      181 gatgaaggct ggcgtctgct ggacttcaaa aatcgtaaag tcggtgagtt tattcaaaaa
      241 ggttaccgca cctgtcgccg ccataccggt gcctatatca ccatcacaca gaacatcgtc
      301 gactttgact ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac
      361 aaaattatcc tcaaacagag tgcgaaggag ttcgcgaaat ataaccagtt attcccggat
      421 cagttccagc cactgcagcg cgacatgatt ggcaagtttg gtgcggccaa agaccagtgg
      481 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgtct gtttgtggac
      541 ccgttaagcc gcgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
      601 cgcagggagg gcctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg
      661 ccggagatgg cttcgctgga agcctggctg gaagaacatg agaaatacag gagtgttgca
      721 tgaccacaac gcaaaaaacg actgatgtta cagccccccg acgcagccac tggtggtgga
      781 cggtgccggg atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca
      841 gactgaacgc accggtgact gccgccttca acatgaaaca gactgtggat gcattttttg
      901 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
      961 acacggcact ggaagccagc ctgcaggcgt ggcagcagaa acaccacgcg gtcattctgg
     1021 tgtcgcctgc cgtggtacag ggggctccgg atatcacccg ggaaatccag caggatattg
     1081 cccggcgaat gagagcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     1141 gcagagtgtg gccgccgccg atcttggcac ctggggcgat ctgtggccgg taaaggagcc
     1201 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     1261 ccggaaaatg gatgccttta aggagcgggt gatccgcaac agtctgcggc cccctgccgt
     1321 gcccggtatc ggacgcacgg agaaatacgg cagccggttg tttgacccgt ccgtcagact
     1381 ggctgcggat atccgggata acgaagggcg ggtgtttgcc cgtcagggtg aagtgatgaa
     1441 tccgctgcag tatgtgcctt ttaaccag
//
LOCUS       DAZFRE010000285         1468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFRE010000285 DAZFRE010000000
VERSION     DAZFRE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.291"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..723
                     /locus_tag="ACHKUI_005252"
     CDS             <1..723
                     /locus_tag="ACHKUI_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="similar to VirB4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6601227.1"
                     /translation="GRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIE
                     NRMYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDF
                     DSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQW
                     FSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKK
                     SGPEMASLEAWLEEHEKYRSVA"
     gene            720..1106
                     /gene="trbI"
                     /locus_tag="ACHKUI_005253"
     CDS             720..1106
                     /gene="trbI"
                     /locus_tag="ACHKUI_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO6601228.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1103..>1468
                     /locus_tag="ACHKUI_005254"
     CDS             1103..>1468
                     /locus_tag="ACHKUI_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6601229.1"
                     /translation="MRCRGLIALLIWGQSVVAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQ"
BASE COUNT          336 a          373 c          450 g          309 t
ORIGIN      
        1 ggccggtatt ttaactctga tgagccgtcc ctgcgggatg atgccagaat ggtggtgctg
       61 gagctgggtg ggctggaaga ccgtccgtca ctgctggttg cggtgatgtt cagccttatc
      121 atttacatcg agaaccggat gtaccgcacg ccgcgtaccc tcaagaaact gaacgtcatt
      181 gatgaaggct ggcgtctgct ggacttcaaa aatcgtaaag ttggtgagtt tattcaaaaa
      241 ggttaccgca cctgtcgccg tcataccggt gcctatatca ccatcacaca gaacatcgtc
      301 gactttgact ctgacaaggc atccagcgca gcccgtgcgg catggggtaa ctcctcctac
      361 aaaattatcc tcaaacagag tgcgaaggag ttcgcgaaat ataaccagtt attcccggat
      421 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
      481 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgtct gtttgtggac
      541 ccgttaagcc gtgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
      601 cgcagagagg gtatgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaaatcgggg
      661 ccggagatgg cttcgctgga ggcctggctg gaagaacatg agaaatacag gagtgttgca
      721 tgaccacaac gcaaaaaacg actgacgtta cagccccccg acgcagccac tggtggtgga
      781 ccgtgccggg atgcctggca atggtgttac tgaacgcagc catcagttat ggcattgtca
      841 gactgaacgc accggtgact gccgccttca acatgaaaca gaccgtggat gcgttttttg
      901 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
      961 acacggcact ggaagccagc ctgcaggcat ggcagcagaa acaccacgcg gtcattctgg
     1021 tgtcgcctgc cgtggtacag ggggcaccgg atatcacccg ggaaatccag caggatattg
     1081 cccggcgaat gagggcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     1141 gcagagtgtg gtcgccgccg atcttggcac ctggggcgat ctgtggccgg taaaggagcc
     1201 ggacatgctg acggtgatca tgcagcgcct gacggcgctg gagcagtccg gtgagatggg
     1261 ccggaaaatg gatgccttta aggagcgggt gatccgtaac agtctgcggc cccctgccgt
     1321 gcccggtatc ggacgcacgg agaaatacag tagccggttg tttgacccgt ccgtcagact
     1381 ggctgcggat atccgggata acgaagggcg ggtgtttgcc cgtcagggtg aagtgatgaa
     1441 tccgctgcag tatgtgcctt ttaaccag
//
LOCUS       DAZFRE010000286         1459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFRE010000286 DAZFRE010000000
VERSION     DAZFRE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.292"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..430)
                     /locus_tag="ACHKUI_005255"
     CDS             complement(<1..430)
                     /locus_tag="ACHKUI_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601230.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKRYELSLFNTTNFGEDP
                     FPDVNLYNNMKSSFFVRFGGTSHPEAWAIYNASTKEVKYIE"
     gene            complement(659..1423)
                     /locus_tag="ACHKUI_005256"
                     /pseudo
     CDS             complement(659..1423)
                     /locus_tag="ACHKUI_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          424 a          326 c          237 g          472 t
ORIGIN      
        1 tttctatata tttaacctct ttcgtacttg cattataaat agcccaagcc tcgggatggg
       61 atgttccacc aaaccttaca aaaaaacttg atttcatatt attatataaa ttaacatcag
      121 gaaaaggatc ttctccaaaa ttagtagtat tgaataatga aagttcgtat cgcttagaaa
      181 attctgttgg taggatagct tcactgccat caatagcatt catccagagt ttgttaccat
      241 tatattccaa caataatctc tgttttgcat gtgtcttatc tcgttcttta acgacagcga
      301 ataccttttt aacaacttgc tgtgcggatg gtcctattgt attaccaaaa gctggcaacc
      361 cgattttgcc ataaacactc tcctgaatga aataaatatc cgttttatcg gcctcgacaa
      421 tatctaccat ttatccctcc aaacaagacc tgacgccagg acaagagcat agtgtgtagt
      481 aaacacttca tgctcttgcc ctgaccattg aaataacaac tttcatttgt ttactgtacg
      541 ctgtctaaat agctgcgtgg aataaagttg cactttttat catcccttgc agagcaagat
      601 tacgaaagcc cgcccccgca aggactgacg cgaggtagtc tctgtccaca gctgcttttc
      661 acatcttaca tcttgcccct gccttgagta tcttatcttt cgttaaaata ttaatagcga
      721 tcttccgtat ccctgaaaat aattatgctg catttcctct tcttattttg aagtcgtctt
      781 tattcattac cacgtccaga tgccagtgca gcttattctc cacgtaccag tgatttcggt
      841 tcgctgtggc gaacttctct gcggttaaat cagcagaaat gctataatat ctgtccgtca
      901 tttctggctc tttcttttgt tctgttatta ttgaacgaaa ggagactgcc atgcataatt
      961 tcttcagctc tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaataa
     1021 tatgaagact gattttaatg acataaatcg ctcccttgcg gcgactcttg tcataagagt
     1081 gccgaagcgt ttttccatca attgcaatga catctttatc atctgaggaa tggcagtcac
     1141 gcatccagtt aataaagcac tcatgaaatt ttgcaggact gatacaggat acaactctgg
     1201 caatgatatc gtgaacagga ataccatttt caaaatcacc atatcacttc aaaaaatcga
     1261 gatgtgtttc cccaaatcct ctatatcttc ccagcattat gcaccagaaa taacggcgca
     1321 aatagttaac agatagaata tctggcaatt tatgttccac tttccaggct tgcctgtaat
     1381 cgggaataat agaaatatgt tccatcaatt ttttaagttc cattttgtcc cccttaatta
     1441 ggcaatgagt atttgatca
//
LOCUS       DAZFRE010000287         1443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFRE010000287 DAZFRE010000000
VERSION     DAZFRE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.293"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            26..>1443
                     /gene="cpsB"
                     /locus_tag="ACHKUI_005257"
     CDS             26..>1443
                     /gene="cpsB"
                     /locus_tag="ACHKUI_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6601231.1"
                     /translation="MIPVILAGGSGSRLWPLSREKFPKQFLKLTGSLTMLQSTLSRLN
                     NLNADDSIVICNEEHRFIVAEQLRELGKLSNNIILEPKGRNTAPAITLAALAAKRKFA
                     DEDPLILILAADHNIQDEHVFCEAINKASSLASYGKLVTFGIVPFKPETGYGYIRRGD
                     EVPVDEQHAVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRP
                     DILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMECTADAVVVPMDAGWSDV
                     GSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVL
                     IADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGE
                     GLSVQMHYHRAEHWVVVAGTAKVTINGDIKLLGENESIYIPLGAMHCLENPGKIDLEL
                     IEVRSGAYLEEDDVIRCYDRYGR"
BASE COUNT          386 a          288 c          394 g          375 t
ORIGIN      
        1 gctaatattc ttttaagagg tgagaatgat acctgttatt ttggctggtg gttcgggaag
       61 tcgcttgtgg ccactttcac gagaaaagtt ccccaagcag tttttaaagt tgactggcag
      121 tttgacaatg ttgcagtcaa cattgtcacg tcttaataat ttaaatgctg atgattcaat
      181 agttatatgc aacgaagagc atagatttat tgttgcagaa caattaagag agttaggcaa
      241 actttcaaat aacattattc ttgaacccaa aggtcgtaat acagcccctg ctataacact
      301 cgcagcatta gcagcaaaaa gaaaattcgc tgatgaagat ccattgattc ttattttagc
      361 tgcagatcac aacatccaag acgaacatgt tttctgtgag gcaattaata aggcgtcatc
      421 tttagctagt tatggaaaac tagtgacttt tggtatcgtt ccattcaaac ctgaaactgg
      481 gtatggctat attcgtcgcg gtgatgaagt gcctgtagat gagcagcatg cggtggcctt
      541 tgaagtggcg cagtttgtcg aaaaaccgaa tctggaaacc gcgcaggcct atgtggcaag
      601 cggcgaatat tactggaaca gcggtatgtt cctgttccgt gccggacgct atctcgaaga
      661 actgaaaaag tatcgtccgg atattctcga tgcctgtgaa aaagcgatga gcgccgtcga
      721 tccggatctc gattttattc gtgtggatga agaggcgttt ctcgcttgtc cggaagagtc
      781 ggtggattac gcggtcatgg aatgcacggc agatgccgtt gtggtgccga tggatgcggg
      841 ctggagcgat gtcggttcct ggtcttcatt atgggagatc agcgcccaca ccgccgaggg
      901 caacgtttgc cacggcgatg tgattaatca caaaactgaa aacagctatg tgtacgccga
      961 atctggcctg gtcaccaccg tcggggtgaa agatttggtg gtagtgcaga ccaaagatgc
     1021 agtgctgatt gccgaccgta atgcggtgca ggatgtgaag aaagtggtcg agcagatcaa
     1081 agctgatggt cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata
     1141 tgactctatc gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga
     1201 aggtttgtcg gtacagatgc attatcatcg cgcggaacac tgggtggttg tcgcgggaac
     1261 ggcaaaagtc actatcaacg gtgatatcaa actgcttggt gaaaacgagt ccatttatat
     1321 tccgctgggg gcgatgcact gcctggaaaa cccggggaaa atagatttag aattaattga
     1381 agttcgctct ggtgcatatc ttgaagaaga tgatgttatt agatgttatg atcgctatgg
     1441 acg
//
LOCUS       DAZFRE010000288         1443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFRE010000288 DAZFRE010000000
VERSION     DAZFRE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.294"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..176
                     /locus_tag="ACHKUI_005258"
     CDS             <1..176
                     /locus_tag="ACHKUI_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFO6601232.1"
                     /translation="DNTLVYSGVIDMPAGRGHMTLEFSVSAWWVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            244..843
                     /locus_tag="ACHKUI_005259"
     CDS             244..843
                     /locus_tag="ACHKUI_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6601233.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSTGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            908..>1443
                     /locus_tag="ACHKUI_005260"
     CDS             908..>1443
                     /locus_tag="ACHKUI_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6601234.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDARPE
                     ALRRFELMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAATSQ
                     TASANSATAAKKSETNAK"
BASE COUNT          365 a          342 c          417 g          319 t
ORIGIN      
        1 tggataatac gctggtttac agtggtgtta ttgatatgcc tgctggtcgc ggccacatga
       61 cgctggagtt ttctgtatca gcatggtggg taaatggctg gtatcccaca gcaagtatca
      121 gcgatttgct ggttgttgtg atgaagaaag ccactgcagg cattacgatt agctgaattt
      181 tataacccca atacgggcgc cagaaatggt gcctttttta ttgcagaaaa gcgagaggta
      241 attatgcgta aagtttgtgc agccattttg tccgcagcca tctgtctgtc cgtatccggt
      301 gcgcctgcat gggcgtctga acatcagtcc acactgagca cagggtatct tcatgcccgt
      361 acgaacgctc ccggcagcga taatctgaac gggattaacg tgaaataccg ttatgagttt
      421 acggacacgc tggggctggt gacgtcattc agttatgcca atgccaaaga tgagcaaaaa
      481 acgcattaca gcgatacccg ctggcatgaa gattccgtgc gtaaccgctg gttcagcgtg
      541 atggcggggc cgtctgtacg cgtgaatgaa tggttcagcg cgtatgcaat ggcgggtatg
      601 gcttacagcc gagtgtcgac cttctccggg gattatctcc gcgtaactga caacaagggg
      661 aaaacgcacg atgtgctgac cggaagtgat gacggtcgcc acagcaatac gtctctggcg
      721 tggggggctg gcgtgcagtt taacccgacc gaatccgtgg ccattgatat tgcttatgaa
      781 ggctccggca gtggtgactg gcgcactgac ggtttcatcg tgggggtcgg ttataaattc
      841 tgattagcca ggtaacacag tgttatgaca gcccgccggt tcaggcgggc ttttttgtgg
      901 agtggatatg gcagcagtaa aaatctcagg tgtgctgaaa gatggtgcgg gaaaaccaat
      961 acagaactgc actattcagc tgaaggcaaa gcgtaacagc accacggtac tggtgaacac
     1021 ggtggcctct gaaaatccgg atgaagccgg gcgttacagc atggatgttg agcatggcca
     1081 gtacagcgtc accctgctgg ttgaaggttt tccgccttca catgccggga ccattaccgt
     1141 ctatgaaggt tccagaccag gtacgctgaa tgattttctt ggtgccatga cggaggatga
     1201 tgcccgtccg gaggcactgc gccgttttga actgatggtg gaagaagcag cacgcaacgc
     1261 tgaagccgcc tctcagagcg cagcggcggc aaagaaatcc gaaactgcag cggcatcatc
     1321 gaagaacgcg gcgaaaacct cagaaacgaa tgcagctaat agtgcacagg cggcagcgac
     1381 ctcacagact gcatcagcaa actccgcgac agcagccaaa aaatcagaaa ccaacgcgaa
     1441 aaa
//
LOCUS       DAZFRE010000289         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFRE010000289 DAZFRE010000000
VERSION     DAZFRE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.295"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1266)
                     /locus_tag="ACHKUI_005261"
     CDS             complement(<1..1266)
                     /locus_tag="ACHKUI_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6601235.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMR"
BASE COUNT          484 a          325 c          256 g          349 t
ORIGIN      
        1 tctcatgatg agtccggtaa tcaggttaag tatcgcggga atgatcgtca ttaacaggca
       61 gatcccccac aacgcctgtg gtgtttgttc tacgccaggt tgatagtcca taaacgctaa
      121 agcgattccg ataaaccctc ccgccagtcc cataccaaac ttttgaaaaa agagaatccc
      181 gccaaatgcc agtccagaaa cacgttgccc cgttttcatt tcaccataat ccactgactc
      241 agcaatggat gcccaataaa tgggaagctg aatatcagtc agaaaggtca ccacaatata
      301 aaatgtaaaa gctaacacta tagaatttgg ttgcacgaaa tacatcgcac cacccaccac
      361 gaacgccagt aattgggtat aacgaaatat ttttattttg tcgtagagtc gagtaagcca
      421 ggtgcaagcg atcatagcca gaacagaggc gatcacgcct gaagttaaaa acggtgaaat
      481 taaagcatcc ccaccattca ggtaatattt tgcataatat gcagcgacag agttacgaat
      541 aatcccgcca aacataatga tcgcaattac agcgcccaaa atgagccatt gatcattttt
      601 gatcagtaac cgaaactgcg cggccatacc gagactgggt tttggatggt agatgcgctc
      661 tctcacgcta aaaaaacaat acaggcataa acacgttgcc agaattccca ttgcccccat
      721 tgctatttga taacccagtt gtacgttatt cttcccaaga tacattgcag ataaaggaac
      781 aataattgtt acggcgaaca tggcaatttt tgtcataaca aaacgataac cattggcgct
      841 caatcgctct tgtggatcat ccgtgataac gctgattaat gaaacataag gaatagcgat
      901 taacgtatag gtcaaggtaa gtaacacata ggtagcccag gcccataaaa gcttaactga
      961 ataatcggta ttaggtgtgg tgaacatcaa ccaaatgctt atcccaaacg gtattgacat
     1021 aaaaagcaac cacggccgat attttcccca acgtgtattc gttctgtctg tcattgttcc
     1081 cacaactgga tcaatcacag catcaaggat acgtacagaa aataacagaa tacctaagtc
     1141 gacgggtttc agaccaaata catccgtgta aaaataagtg atgatcatgg ccaaagacat
     1201 cattactatc gcgatagcca tatcaccagc accaaaacct atcttttcat ttattgtaag
     1261 tttcatacac agcactcatt gattacgtga acttatatta aaaacaaata tatctgaact
     1321 cattcagcac ataactttac gagtgagcat aaatgcattg gttgatacca caacaaacca
     1381 ttgagtcaat attgagcaac atcacataaa aacc
//
LOCUS       DAZFRE010000290         1388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFRE010000290 DAZFRE010000000
VERSION     DAZFRE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.296"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..189
                     /locus_tag="ACHKUI_005262"
     CDS             <1..189
                     /locus_tag="ACHKUI_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6601236.1"
                     /translation="LMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKLGRSSRW
                     LKSEVEAWLQARIAQSRP"
     gene            275..847
                     /locus_tag="ACHKUI_005263"
     CDS             275..847
                     /locus_tag="ACHKUI_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601237.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            1056..1163
                     /locus_tag="ACHKUI_005264"
                     /pseudo
     CDS             1056..1163
                     /locus_tag="ACHKUI_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1173..>1388)
                     /locus_tag="ACHKUI_005265"
     CDS             complement(1173..>1388)
                     /locus_tag="ACHKUI_005265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601238.1"
                     /translation="INVLVKIARMTVFADSVQWKRYYIIYLSNKFNTNFRRCVGELMT
                     NFQRGMEKCMESHKEKIVIVQLTFISG"
BASE COUNT          372 a          347 c          305 g          364 t
ORIGIN      
        1 ctgatggatg accagatggt cgacatggcg tttatcactc aactgaccgg cttaaccgat
       61 aagtggtttt acaagctcat caaggatggt gcctttccgg cccccatcaa actcggccgc
      121 agctcccgat ggctgaaaag tgaagtggaa gcctggttgc aggcgcgtat tgcacaatcc
      181 cgtccgtaat tcctgaccat tacccgttca accgcagcga atacactatc cggcctccgg
      241 aggcattttg ctgcgtgcta aaggagagaa tattatgcaa aaacttgaaa accggtgtga
      301 tctgctgctc acccagcatc agaaatggat ggcaagtgta acccggttta ttgtcgctca
      361 cggaatgggc tctccgcatc ttcatggtta tcatcgcctg accctggccc acttttttct
      421 gccggaaaaa ggcaccatca tctcggtggc accacaggga ctctatcagg tagtcaaccc
      481 ggcaacgcca ccgttcattc ctgccattca ggaagggtta atgaccagca ttcagactca
      541 tgaaatcatg ttacttacac attttaacct gggtggtgtt ttactcagtg aactacatcg
      601 tctgggagaa agccgactgg caaacaggct gaacagtctg ctccggcgtt ttgacgatcg
      661 cgatctctac cacaccctga tatggctgtg ctggtatgat ctgatgtgcg cccactccat
      721 gcagccctgg actgaagagt taaagcataa atcacacgca gaacttgaaa gctgggctgt
      781 ggcgcgcaaa cgggaaaagc gcgagcttga actgatgatt gacgagtacc tgttatacgc
      841 ctgttgattc ataatcactc ccgggcggta cgtagtcagt gataccggaa tttccggaga
      901 gggtcgcagg tatattgttt ctgtgcaatt catggttatt cacgttggat gtagtgattg
      961 ctgcatccaa tgctatcgga aagagattaa ccagcaaatg gttcctcaaa tcgtattgat
     1021 atttacccgg cgcattgagc accaccatct gcccccgttc ccacatcaaa cggctggacc
     1081 ggaaaacgat ttgcttttca tgctccgtgg aaattcacga aaaagttatt gggtcattca
     1141 tcgaaaaaaa catattctgc tgactggagt catcacccag aaataaacgt caactgaaca
     1201 ataacgattt tttctttgtg ggattccata cacttttcca tccccctctg aaaattagtc
     1261 atcaactcac caacacatct tctgaaattt gtattaaact tattgcttaa atagatgata
     1321 taatatctct tccattgcac ggaatcagca aaaactgtca ttctggcaat cttaacaaga
     1381 acatttat
//
LOCUS       DAZFRE010000291         1387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFRE010000291 DAZFRE010000000
VERSION     DAZFRE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.297"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..328)
                     /locus_tag="ACHKUI_005266"
     CDS             complement(<1..328)
                     /locus_tag="ACHKUI_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6601239.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWE"
     gene            747..995
                     /locus_tag="ACHKUI_005267"
     CDS             747..995
                     /locus_tag="ACHKUI_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6601240.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            992..>1387
                     /gene="umuD"
                     /locus_tag="ACHKUI_005268"
     CDS             992..>1387
                     /gene="umuD"
                     /locus_tag="ACHKUI_005268"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6601241.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFG"
BASE COUNT          344 a          386 c          371 g          286 t
ORIGIN      
        1 cttcccagcg acgctgtcca gtaccgctct gcagccagag ttcatgcagc atcaggcagt
       61 tttgctcatc cagcagtaca ccgttctgca ggtgtgtcag ccagcgggtg aacgcgatat
      121 tttccggtga tacagcacca cgtccggcag ccgtcactcc ggggcactgc tcagagcgga
      181 tattttcagt gatctgcaga agtccggcac ttccggccag aaacagctga ataccttcat
      241 ttgtggcgcg gcgataaagc ggtgtaactg caccgctggc cagtcgttta atctcagcga
      301 tctgtgccgg ttcaccagaa aaatacatac gattcgagca ccagtttggc atgggaattc
      361 tcctctcaca tgcggcctcc atcaggccgt gccgcacctg ttaccgggcg accgcatacg
      421 aatgcaggtg cggccccgaa gccgcagcgc gtggaggagg aacgacgaaa cgtgaccggg
      481 tgaggaggcc ggcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca
      541 cggcctgggt aaccaggtat tttgtccaca gaaccgtgca caaaatgctg tggataagct
      601 ggacacagca gcccacagca ggcatacagc cacacactca gagaattgag cgttttttag
      661 tcacaacaat gacatttgac tgtgtgcgga ttcagttatt agaatactgt ataaacatac
      721 agcataaaga aaagggagat gagaacatga tccgcattga aattcttttc gaccgccaga
      781 gcacaaaaaa cctcaaatca ggcacacttc aggcgctaca gaatgaaatc gaacaacgtc
      841 tgaaaccaca ctacccggaa atctggctgc gtatagacca gggcagcgcc ccgtctgtat
      901 ctgtcacagg agcccgcaac gacaaggata aagaacgtat cctgtctctg ctggaagaaa
      961 tctggcagga cgacagctgg ctgcctgcag catgagtacc gtttatcacc gtccggctga
     1021 cccttcaggc gatgacagtt acgtgcgacc gttgtttgcc gatcgttgcc aggccggttt
     1081 tccttcgcct gccactgatt atgctgagca ggaactggat ctgaacagct attgcatcag
     1141 cagacctgca gccaccttct ttctgcgcgc cagcggtgaa tcgatgaacc aggctggcgt
     1201 gcagaatggt gatctgctgg tagtggacag ggccgaaaaa ccacaacacg gggacatcgt
     1261 tatcgctgag atcgacggtg agttcaccgt caaacgactg ctgttgcgcc cacgcccggc
     1321 actggagccg gtttcagaca gcccggagtt ccgcacactg tatccggaaa acatctgtat
     1381 ttttggt
//
LOCUS       DAZFRE010000292         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFRE010000292 DAZFRE010000000
VERSION     DAZFRE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.298"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1332
                     /locus_tag="ACHKUI_005269"
     rRNA            <1..1332
                     /locus_tag="ACHKUI_005269"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          336 a          315 c          426 g          278 t
ORIGIN      
        1 gggcctcttg ccatcggatg tgcccagatg ggattagcta gtaggtgggg taacggctca
       61 cctaggcgac gatccctagc tggtctgaga ggatgaccag ccacactgga actgagacac
      121 ggtccagact cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat
      181 gcagccatgc cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg
      241 aagggagtaa agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa
      301 ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg
      361 taaagcgcac gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc aacctgggaa
      421 ctgcatctga tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg
      481 gtgaaatgcg tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac
      541 tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc
      601 cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga gctaacgcgt
      661 taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat tgacgggggc
      721 ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc ttacctggtc
      781 ttgacatcca cggaagtttt cagagatgag aatgtgcctt cgggaaccgt gagacaggtg
      841 ctgcatggct gtcgtcagct cgtgttgtga aatgttgggt taagtcccgc aacgagcgca
      901 acccttatcc tttgttgcca gcggtccggc cgggaactca aaggagactg ccagtgataa
      961 actggaggaa ggtggggatg acgtcaagtc atcatggccc ttacgaccag ggctacacac
     1021 gtgctacaat ggcgcataca aagagaagcg acctcgcgag agcaagcgga cctcataaag
     1081 tgcgtcgtag tccggattgg agtctgcaac tcgactccat gaagtcggaa tcgctagtaa
     1141 tcgtggatca gaatgccacg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca
     1201 ccatgggagt gggttgcaaa agaagtaggt agcttaacct tcgggagggc gcttaccact
     1261 ttgtgattca tgactggggt gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg
     1321 atcacctcct taccttaaag aagcgtactt tgaag
//
LOCUS       DAZFRE010000293         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFRE010000293 DAZFRE010000000
VERSION     DAZFRE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.299"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..797)
                     /locus_tag="ACHKUI_005270"
     CDS             complement(<1..797)
                     /locus_tag="ACHKUI_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /protein_id="HFO6601242.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANT"
     gene            complement(848..>1341)
                     /locus_tag="ACHKUI_005271"
     CDS             complement(848..>1341)
                     /locus_tag="ACHKUI_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601243.1"
                     /translation="KISFPEKSGSNLLKPEAVSCNLLSLCVLAPVFCTPRKLAEVSRE
                     IIVASAQEIAWTPGSSRIDGHQAENITWAISSQSPADKYDLINKTSCVSPDSGRFYEP
                     DTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKVSSGRTAGGHHHRHTGYTWSV
                     SVN"
BASE COUNT          360 a          356 c          317 g          308 t
ORIGIN      
        1 gtgttcgcgg tattatcaaa atccggctgt gtcaggcact ccttccacaa atcccatgcg
       61 gcacagcggg catccagcgg attcaggatt ttatcgatgg agggatcata gtaactttta
      121 acaaattccc ctgaacggtc ataaatcacc accatatcac cgcgctgacg ggcatagttg
      181 gccagacgac ggataacctc cgacttaccg gccccgaccg tgccgtgcag acagaagttc
      241 tggatttcgg aatcccggat aatcggcagg tcgccaatcc ggatatcgga gtccttgccg
      301 tcttttttca gcatccgggc aacgtctttc ggattgtctg tcagctgacg accacctgtg
      361 acttcatttt cgctctgttg tttaccctga cgccccagaa tccaggagac aacaaagaag
      421 gtgatcaggc aaataaccag ggccacgaca gacgccagaa cgaatgcaga ccatagctgc
      481 tctccgcacc agatcatata tttatcatgc agtacctggg cagcgttcat ccggaaggtt
      541 ttgccgtaat actggatctc atagaccggc tgggatttta ttaaatcccg catgccttcc
      601 agtgacgtac accaccagta aatacagccg ttgataaacg tctgccagct tatttttacc
      661 cataagacca gaccaatgag gatccagaaa aaaataaaca ggcagtaaag catgatattg
      721 gcgatctggc tgaacatgcg gatacgcatg gacgcaatct gaccgccctg ggtcatatcc
      781 tttgcgttaa aactcatgat gaaactccgg gaaaatctgg aacagggcag aaaaggatga
      841 tgtggggtta attcacagaa acagaccagg tgtaccctgt atgccggtgg tgatgcccgc
      901 ctgcagtcct gccggaagag accttctctg taacaatgtc agtcaggcgc aatacttcag
      961 accagtcact cccatgcgga aactggatct gaataaaaag gcgtacccgt tcacggtgat
     1021 ccagaactgt gtccggttca taaaaacggc cactgtcagg agaaacacag gacgttttat
     1081 tgatgagatc atacttatcg gcaggagact gcgatgaaat cgcccaggtg atattctccg
     1141 cctggtggcc atcaatacga ctgctccccg gcgtccatgc aatttcctgc gcactcgcca
     1201 cgatgatctc cctggaaact tcagccagct ttctgggtgt acagaatacc ggtgccagca
     1261 cacacaggga caacaaatta cagctgaccg cctccggttt aagcaaattg ctcccggatt
     1321 tttcaggaaa agatattttt g
//
LOCUS       DAZFRE010000294         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFRE010000294 DAZFRE010000000
VERSION     DAZFRE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.300"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..288)
                     /gene="traA"
                     /locus_tag="ACHKUI_005272"
     CDS             complement(<1..288)
                     /gene="traA"
                     /locus_tag="ACHKUI_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO6601244.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTCLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGQETVKATFGKNSSIVKWIVLAEVLVGAVMYMMTKN"
     gene            complement(322..549)
                     /gene="traY"
                     /locus_tag="ACHKUI_005273"
     CDS             complement(322..549)
                     /gene="traY"
                     /locus_tag="ACHKUI_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO6601245.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(643..1329)
                     /locus_tag="ACHKUI_005274"
     CDS             complement(643..1329)
                     /locus_tag="ACHKUI_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFO6601246.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
BASE COUNT          431 a          308 c          218 g          384 t
ORIGIN      
        1 gtttttggtc atcatgtaca tgaccgcacc gaccagcacc tcagccagaa caatccattt
       61 aacgatactg gagttcttac cgaaggtcgc cttaaccgtt tcctgacctt tagccatcag
      121 gtcgccttta ccagcagcca tagctaactc agggaacaac atcatcagaa cagcaaccgg
      181 gatcactgca cgaaccaggc gaagcatatt cagacaagtg aatttagaaa aaaacgattt
      241 cttttcaacg ggcgcagaag caccctgaac acttaaaaca gtattcatat gattcctctg
      301 attaatattg acagatgaaa actaaagttc tttaaatgtt gactcgttct cttcgatcac
      361 ctccctgaat atttcttcat tatagaaatc aggaaaccgt ttcagatgat ctctgagacg
      421 gatctgaacc tctatagttt tacttctacc gctacgatct ttggcccgaa taagtcggtt
      481 atttgtatct tcatcgagat acactgaaac tgttctgctt atcccgcctc tcgcgttacg
      541 cctcctcact caattcacct ctgtttcccg actaataaaa tcatgattgc acagaaaccg
      601 acgaaaaaaa acacgccaaa attggcgcac cttatttaac atttacttaa caccataaaa
      661 ttcacgaatt atacttaata gcgaatctat ttctccttta tagatacatg acactctgaa
      721 taaatcaaag tgttcaaaaa attcttttat tttccgttta actggtttaa ttcgatcctc
      781 agatgctctg tctgtaatac ccaactctct ggcaattgtt gcgtgaccaa cacccagcat
      841 ataaaaagag aaaacaacca atgtccctgg agaaagatca ttccatatcg ttaatttatc
      901 agaagtattt gtattacttc cactctgatt taacgcaaaa aatggataat caggaaacgg
      961 attaatttgc cataaaacaa cagattcgtc attataaaaa gaaacattct ccatccttat
     1021 ctgatagatt tcgccatgaa aattaaaacg acgacaaaat gcagagccat gtccaagagc
     1081 ctggcattcc agttcaagtg atgaaagaaa aatttcaacc tgcagacgta tataactctc
     1141 tccggataag ggtttatctt ctctggaaaa gagttcaata aaagccccat tggcataaag
     1201 gattttccgg tttttgttac ggacacaaac cggaacggga gaatttctgt agatattctg
     1261 aacgttaagg atgtatttat ttacagattg actgttaagt ggcctttctc tacgatccag
     1321 cgcacacata ggaacctccg t
//
LOCUS       DAZFRE010000295         1334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFRE010000295 DAZFRE010000000
VERSION     DAZFRE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.301"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..797)
                     /locus_tag="ACHKUI_005275"
     CDS             complement(<1..797)
                     /locus_tag="ACHKUI_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /protein_id="HFO6601247.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANT"
     gene            complement(848..>1334)
                     /locus_tag="ACHKUI_005276"
     CDS             complement(848..>1334)
                     /locus_tag="ACHKUI_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601248.1"
                     /translation="SFPEKSGSNLLKPEAVSCNLLSLCVLAPVLCTPRKLAEVSREII
                     VASAQEIAWTPGSSRIDGHQGENITWSLSSQSPADKYDLINKTSRVSPDSGRFYEPDT
                     VLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKVSSGRAAGGYHHRHTGYTWSVSV
                     N"
BASE COUNT          360 a          353 c          313 g          308 t
ORIGIN      
        1 gtatttgcgg tattatcaaa atccggctgt gtcaggcact ccttccacaa atcccatgcg
       61 gcacagcggg catccagcgg attcaggatt ttatcgatgg agggatcata gtaactttta
      121 acaaattccc ctgaacggtc ataaatcacc accatatcac cgcgctgacg ggcatagttg
      181 gccagacgac gaataacctc cgacttaccg gccccgaccg tgccgtgcag acagaagttc
      241 tggatttcgg aatcccggat aatcggcagg tcgccaatcc ggatatcgga gtccttgccg
      301 tcttttttca gcatccgggc aacgtctttc ggattgtctg tcagctgacg accacctgtg
      361 acttcatttt cgctctgttg tttaccctga cgccccagaa tccaggagac aacaaagaag
      421 gtgatcaggc aaataaccag tgccacaact gtggccagaa cgaatgcgga ccatagctgc
      481 tctccgcacc agatcatata tttatcatgc agtacctggg cagcgttcat ccggaaggtt
      541 ttgccgtaat actggatctc atatactggc tgggacttga ttaaatcccg catgccttcc
      601 agcgtggtac accaccagta aatacagccg ttcacaaacg tctgccagct tatttttacc
      661 cataaaatca gaccaacgag tatccagaaa aaaataaaca ggcagtaaag catgatattg
      721 gcgatctggc tgaacatgcg gatacgcatg gacgcaatct gaccgccctg ggtcatatcc
      781 tttgcgttaa aactcatgat gaaactcagg gaaaatctgg aacagggcag aaaaggatga
      841 tgtggggtta attcacagaa acagaccagg tgtaccctgt atgccggtgg tgatacccgc
      901 ctgcagccct gccggaagag accttctctg taacaatgtc agtcagtcgc aatacttcag
      961 accagtcact cccgtgcgga aactggatct gaataaaaag gcgtacccgt tcacggtgat
     1021 ccagaactgt gtccggttca taaaaacggc cactgtcagg agaaacacgg gaggttttat
     1081 tgatgagatc atacttatcg gcaggagact gcgatgacag agaccaggta atattttcgc
     1141 cctgatgacc atcaatacga ctgctccccg gcgtccatgc aatttcctgc gcactcgcca
     1201 cgatgatctc cctggagact tctgccagct ttctgggtgt acagagtacc ggtgccagca
     1261 cacacaggga caacaaatta cagctgaccg cctccggttt aagcaaattg ctcccggatt
     1321 tttcaggaaa agat
//
LOCUS       DAZFRE010000296         1332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFRE010000296 DAZFRE010000000
VERSION     DAZFRE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.302"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1083
                     /gene="tssI"
                     /locus_tag="ACHKUI_005277"
     CDS             <1..1083
                     /gene="tssI"
                     /locus_tag="ACHKUI_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6601249.1"
                     /translation="TRFTLTGHPQKMLNREWQVVQSILSGDQPQALHGSQGRGTTLGN
                     QLEVIPADRTWRPRQQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVKFHWDRYNPAT
                     EESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRTYHEDNRSPGD
                     LPGTKTQMTIRSKTYKGSGFNELRFEDATGGEQVYIHAQKNMDTEVLNNRTTDVKADH
                     TETIGNDQKITVGLGQTVNVGSKKEGGHDQKVTVANDQHLTIKNDRHKVVNNNQTSKV
                     TGTDTEEVVKKQSIKIGDNYELKVEHGTNIISGDSIELICGQGESGTCSIKLEKTGKI
                     IIRGTEFLFEATGPVDIKGKDIHLNG"
BASE COUNT          394 a          250 c          406 g          282 t
ORIGIN      
        1 acgcggttta cgctgacggg gcatccgcag aagatgctga accgtgaatg gcaggtggta
       61 cagagcattc tgtcaggaga ccagccgcag gcgcttcacg gcagccaggg gagaggaacc
      121 acgctgggca atcagttaga ggtgataccg gcggaccgga cgtggcgtcc acggcagcaa
      181 agcaaaccga aggtggacgg gccacagagc gccattgtca ccggtcccgc aggagaggaa
      241 atcttctgcg acgaacacgg gcgggtacgg gtgaagtttc actgggacag gtataacccg
      301 gcaacggagg agagttcgtg ctgggtgagg gtgtcacagg cgtgggcggg gccgggattt
      361 ggtaatctgg cgataccgcg ggtgggccag gaggtgattg ttgatttcct gaacggggac
      421 ccggaccagc cgctggtcat gggcaggacg taccatgagg acaaccgttc accaggggac
      481 ctgccgggga cgaagacgca gatgacgata cggtcgaaga cctataaagg aagcggcttt
      541 aatgaactgc ggtttgaaga cgccacgggc ggtgaacagg tctatatcca cgcgcagaag
      601 aacatggata cggaggtgct gaataatcgg acgacggatg tgaaggctga ccacacggag
      661 accattggta acgaccagaa gataacggtg gggttgggcc agacggtgaa tgtggggagc
      721 aaaaaggaag gtggtcatga ccagaaagtg acggtagcga acgatcagca tctcacgata
      781 aaaaatgacc ggcacaaggt tgtaaataac aaccagacaa gcaaggtgac gggaactgat
      841 accgaagaag tcgtcaaaaa acagagtatt aaaataggtg ataattacga actgaaagtt
      901 gagcatggta caaatatcat atctggggac agtattgagc taatttgtgg gcagggagag
      961 agcgggactt gttccataaa actggagaaa acaggaaaga taataatacg ggggacggag
     1021 tttctatttg aggctaccgg tcctgtagat attaaaggaa aagatattca tctgaacgga
     1081 tagtgcagga tataaaacat ggtgattgta tttgctttat acgcctgtga atcaccattt
     1141 gtgattgttt aaattaatca tagcatgact tattcaggaa tcaataagca tgatagaaat
     1201 gaaaaataac acgccatttc ctttgttgtc atttgaaaaa tatggtcgtt atggtctttt
     1261 gtttgatgtt attgctatta aaatgtcatt gcgaattaaa aatggatttt atgctgatct
     1321 tgctgagttt ca
//
LOCUS       DAZFRE010000297         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFRE010000297 DAZFRE010000000
VERSION     DAZFRE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.303"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            161..763
                     /gene="ais"
                     /locus_tag="ACHKUI_005278"
     CDS             161..763
                     /gene="ais"
                     /locus_tag="ACHKUI_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6601250.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(802..1227)
                     /gene="nudI"
                     /locus_tag="ACHKUI_005279"
     CDS             complement(802..1227)
                     /gene="nudI"
                     /locus_tag="ACHKUI_005279"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6601251.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          367 a          290 c          277 g          392 t
ORIGIN      
        1 tatatttttt cttatttaga ctttgcattt ggcaaaattt tgaggcattt tgccggcatc
       61 ataggatttt taatattaca ccaactgcta attatcgcca gaaaagtcgc tcaatttcac
      121 ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt tttgccgatc
      181 ttcgttgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcga ttgccggact
      241 gggtactcat gccgcctgga gtagcaatgg tttgccacgc atcgacaata aaacactggc
      301 cagactggca cagcagcacc cggttgtcgt tttgtttcgc catgctgaac gttgcgatcg
      361 ttcaaccaat caatgcttgt cagataaaac aggtattacg gtcaaaggta cccaggatgc
      421 ccgtgaactg ggcaacgctt ttagtgctga tatccctgat tttgatctct attccagtaa
      481 taccgtccgg actattcagt cggcgacctg gttttcggcg ggtaaaaagc tgacggtaga
      541 taaacgattt cttcaatgcg gtaatgagat ttatagtgca attaaagact tacaacgcaa
      601 agcacctgat aaaaatatcg ttatttttac ccataatcat tgcctgacat atattgctaa
      661 agataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca tgcatgtgga
      721 aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca atctgacagc
      781 cagtgatggc tgtcatcatt gttaaagaag acctttcaaa cgtaacgttt ttcgggtggc
      841 aacattcaaa tcataatgca ccagatcttc aggttttacc cacgcgtagt cctgaaactc
      901 ttcgtttatt ttcacatctc ggttggcaga aacgcagtca aaaatcaggt aaatcatata
      961 aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc tgaaggtcca
     1021 cggcgtgatt tctgtcaaaa gcagctgttc tcccagttct tcgcgaattt cgcggcgtag
     1081 tgcctcttca attcgttcgc caggctccac gccgccaccc gaaagcgccc attgaccggg
     1141 gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa gcaccatcat tttgaatcaa
     1201 agggcataca atagtccgtt gtcgcacagg ggtattcctt ataaataaaa agaaaccatg
     1261 agaatacggt aactcagaaa tatgtgaaag tgacagtaat ctcttttgcg atgataacta
     1321 cattta
//
LOCUS       DAZFRE010000298         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFRE010000298 DAZFRE010000000
VERSION     DAZFRE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.304"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            66..152
                     /locus_tag="ACHKUI_005280"
                     /pseudo
     CDS             66..152
                     /locus_tag="ACHKUI_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            149..1300
                     /locus_tag="ACHKUI_005281"
     CDS             149..1300
                     /locus_tag="ACHKUI_005281"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO6601252.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          424 a          286 c          310 g          293 t
ORIGIN      
        1 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
       61 gagcgagata tattaaaaaa agcaactgta gattcaattg gtcaacgcaa cagttatgtg
      121 aaaacatggg gttgcggagg ttttttgaat gagacgaaca tttacagcag aggaaaaagc
      181 ctctgttttt gaactatgga agaacggaac aggcttcagt gaaatagcga atatcctggg
      241 ttcaaaaccc ggaacgatct tcactatgtt aagggatact ggcggcataa aaccccatga
      301 gcgtaagcgg gctgtagctc acctgacact gtctgagcgc gaggagatac gagctggttt
      361 gtcagccaaa atgagcattc gtgcgatagc tactgcgctg aatcgcagtc cttcgacgat
      421 ctcacgtgaa gttcagcgta atcggggcag acgctattac aaagctgttg atgctaataa
      481 ccgagccaac agaatggcga aaaggccaaa accgtgctta ctggatcaaa atttaccatt
      541 gcgaaagctt gttctggaaa agctggagat gaaatggtct ccagagcaaa tatcaggatg
      601 gttaaggcga acaaaaccac gtcaaaaaac gctgcgaata tcacctgaga caatttataa
      661 aacgctgtac tttcgtagcc gtgaagcgct acaccacctg aatatacagc atctgcgacg
      721 gtcgcatagc cttcgccatg gcaggcgtca tacccgcaaa ggcgaaagag gtacgattaa
      781 catagtgaac ggaacaccaa ttcacgaacg ttcccgaaat atcgataaca gacgctctct
      841 gggacattgg gagggcgatt tagtctcagg tacaaaaaac tctcatatag ccacacttgt
      901 agaccgaaaa tcacgttata cgatcatcct tagactcagg ggcaaagatt ctgtctcagt
      961 aaatcaggct cttaccgaca aattcctgag tttaccgtca gaactcagaa aatcactgac
     1021 atgggacaga ggaatggaac tggccagaca tctagaattt actgtcagca ccggcgttaa
     1081 agtttacttc tgcgatcctc agagtccttg gcagcgggga acaaatgaga acacaaatgg
     1141 gctaattcgg cagtactttc ctaaaaagac atgtcttgcc caatatactc aacatgaact
     1201 agatctggtt gctgctcagc taaacaacag accgagaaag acactgaagt tcaaaacacc
     1261 gaaagagata attgaaaggg gtgttgcatt gacagattga atctacaacc gcg
//
LOCUS       DAZFRE010000299         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFRE010000299 DAZFRE010000000
VERSION     DAZFRE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.305"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..740
                     /locus_tag="ACHKUI_005282"
     CDS             <1..740
                     /locus_tag="ACHKUI_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601253.1"
                     /translation="DDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDK
                     SLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARLA
                     ALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTREYGHTSRSPLDTLESAIG
                     FHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKDN
                     RWVPGWMCAPRPQTDATERTDNLADAA"
     gene            795..1229
                     /gene="psiB"
                     /locus_tag="ACHKUI_005283"
     CDS             795..1229
                     /gene="psiB"
                     /locus_tag="ACHKUI_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6601254.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            1226..>1299
                     /locus_tag="ACHKUI_005284"
     CDS             1226..>1299
                     /locus_tag="ACHKUI_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="PsiA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6601255.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAET"
BASE COUNT          310 a          354 c          396 g          239 t
ORIGIN      
        1 ctgacgacgc tgaccgcacg gaacaggtgc aggaaaaagc gtcagtggag gaaatcagtc
       61 tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggca gcactgatgc
      121 agcaaccgga caaatccctg acactgctgg catggacgct ctgcctgaat gtgtttggca
      181 gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat tcgctgacca
      241 gcgatgcgcc atcggggaag gaaggtgccg cattcatggc gctgatggca gaaaaagccc
      301 gtcttgctgc cctgttaccg gagggatggt cacgggacat gacgacattc ctgtcactca
      361 gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt aacggtgtcc
      421 agacccgtga gtatggtcac acgtcacgca gtccgctgga cacgctggag agcgccatcg
      481 gctttcacat gcgcgactgg tggcagccga cgaaagcaaa ctttttcgga cacctgaaaa
      541 agccgcagat tatcgcagcc ctgaatgagg caggactatc cggtgccgca cgggacgcgg
      601 agaagatgaa gaaaggcgat gcggctgaac atgcagagca ccatatgaaa gacaaccgct
      661 gggtgcctgg ctggatgtgt gcaccacgcc cacagacgga tgccactgaa cgcaccgata
      721 acctggctga tgccgcctga tgaacaacca catcgccccg ccggagacgg ggcggcagca
      781 agggagatac cgtgatgaaa actgaactga ctctgaatgt attacatacc atgaacgcac
      841 aggaatatga agatatccgg gctgccggaa gtgatgaacg tcgtgagctg acacacgccg
      901 tgatgcggga actggatgcg ccggacaact ggacgatgaa cggcgagtac ggcagcgagt
      961 ttggcggctt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt tttcacctgg
     1021 cgttatgttc accgggcgat gtctcccagg tctgggtgct ggttctggtg aatgccggtg
     1081 gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagcc gtcagccatt
     1141 cactggcact ggcggcgtca ctggatacgc agggatacag cgttaacgac atcatccata
     1201 tcctgatggc ggaaggaggt caggtatgag cgcacgttca caggcactgg ttcccctcag
     1261 cacagagcaa caggccgcat ggcgggcagt ggcagagac
//
LOCUS       DAZFRE010000300         1279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFRE010000300 DAZFRE010000000
VERSION     DAZFRE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.306"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..314
                     /locus_tag="ACHKUI_005285"
                     /pseudo
     CDS             1..314
                     /locus_tag="ACHKUI_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            987..>1279
                     /locus_tag="ACHKUI_005286"
     CDS             987..>1279
                     /locus_tag="ACHKUI_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601256.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLG"
BASE COUNT          317 a          319 c          390 g          253 t
ORIGIN      
        1 gacatcatcc tgataaacgc agtgcccgtg cgcagcgcga tgactggctg aagaaagaga
       61 tactgcgcgt atacgatggg aatcatcagg tatacggtgt gcgtaaagtc tggcgtcagt
      121 tgttacggga aggaatcagg gtagccagat gtacagtggc gcgcctcatg gcggttatgg
      181 gacttgccgg tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaagccgttg
      241 ccgcaggcga ccgcgtaaac cgtcagtttg tggcagaacg tcctgaccag ttgtgggtgg
      301 ctgattttac ttacggtgtg cccggagttc agggcgggca tggatgctta aatgaaccgc
      361 gagtctgtct ggaatattga accggtaact cacgatgaga aacccaacaa tcccaccggg
      421 tgtgacggtg gagaacctga gcggcagtga cctgcggcat gcccgcaggg tgatgtaacc
      481 cgctgacaac ggggattgag gcgagatcac taagccgaga tgatcctcaa ggttaagtac
      541 tgaaaggctg aagaacatga acccgttaat ccgcctctgt gggttgaaaa cgtcaccacg
      601 gcctacgtga tctgacaggc cgtgcaggag gaactggcag tgatacgtaa gcactaccgg
      661 tcgaaggtgt tttgacatgt atgcgaaaca ccggggcagc agcgtctatc acgcttgcgt
      721 tgctgacttc tgccaacttg cggcaagcaa ggataaagag tgcgacgggc agcctcctca
      781 gtatgcctga gtccaggcag gtaaaccggg gaaggtcagc gacggatgtt aagggggcat
      841 ggctccgatg acgcgctggc tggcggagct tccgtagtag tccgcgatgg ggaaagccca
      901 ttacatggcg aagggaagca gtttgaatgt gtttgcgacg tgaattaact gacctaacga
      961 ggtgaagacc tttgataatc agcgaaatgc aacgcaagct tgccacatgg gcagccaccg
     1021 atccgtccct acggattcaa cggctgctgc gtctgataac acaaccagaa tggctggctg
     1081 aagcggcgcg gatcacgctt tcatcaaagg gggcccatac ccccggcgtt gatggcgtga
     1141 acaaaacaat gctacaggcc agactggctg ttgagctgca aatcctcagg gatgaattac
     1201 tctcaggcca ctaccagccc ttgcccgcca gacgggttta catccctaaa agcaacggca
     1261 aactgcgacc actgggtat
//
LOCUS       DAZFRE010000301         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFRE010000301 DAZFRE010000000
VERSION     DAZFRE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.307"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..330
                     /locus_tag="ACHKUI_005287"
     CDS             <1..330
                     /locus_tag="ACHKUI_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6601257.1"
                     /translation="ASVYALAKKMMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGA
                     NWFDRTVSADAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWRHIEFHPECN
                     AIYAALD"
     gene            377..799
                     /locus_tag="ACHKUI_005288"
     CDS             377..799
                     /locus_tag="ACHKUI_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6601258.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            796..987
                     /locus_tag="ACHKUI_005289"
     CDS             796..987
                     /locus_tag="ACHKUI_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601259.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT          269 a          315 c          403 g          285 t
ORIGIN      
        1 gcgtctgtat acgcgctggc gaaaaaaatg atgccggaat atgaaggcgg attctggcac
       61 ttcatccgcc tgccggacgg tggcggttac atgatgccgg atggcgaccg tttccacatg
      121 gtgaacggtg caaactggtt tgaccgtacg gtcagcgccg atgccgcagg catcattctg
      181 acttcccttg tgattaaccg ccagttgtgg ctgtaccacg acagcgggga tgcaggactg
      241 acccatcttt accggatgcg cgatgcgcag ttgtggcgtc acatcgaatt tcaccccgaa
      301 tgcaacgcga tttacgcggc actggactga ttaacggacg gggcggcaat gccgcccctg
      361 taaaggagac gagaacatgt actgtactgt taaagaaatc atccgcgatg tactggatac
      421 agacgtgccg gacagtgaat gcgtttttgc cgtggtgctg acccgtgggg atgtgcgcta
      481 catagcccag gactggaacc tgtcagacga tgagctggaa accgtcatgc agcggctgga
      541 cgatgccttt gaatatggtg cggatgtcag cattgttcac gacgttgttc gtgagctgat
      601 ggaagaaaag cgagccagcc gtcaggtgac agttccggcg gtgatgctgg aaaaagtgat
      661 ggcgctggca ggcagtgaaa tgaagcgcct gtatgccgtc gggagtgaga acgggggcga
      721 cggtgacgca ttcgtcaggg aagaacgcga agcaatggac gttgtgttac aggcgctgga
      781 cggggagcac atgtcatgaa tatcagcaca gaaactcgcg aaattctgcg taattacagg
      841 gccgtgatta atgcgcggcg tcgtgaaatg gggcagaaac cgctcaccac cgcgcagatt
      901 gttgatgaaa tctgcgattt tgtggcgaat cagcaggcgg ttttcctggg cggtcactac
      961 atcttacagg gcagcagaaa caggtgatac aggggcaggc ggtggaggcc gcctgtcagc
     1021 ccgcgcgaaa acccctggca ggctgacgcc tgccggaagg gaagcgggcc acgctggcgc
     1081 gtcgcccgtt acccttcccg tctgtgatgt gtctgactca ggagtgtttc tttcttatgt
     1141 tatggaactg ttcctgatgt tgttgttgtt gttcctttat ctgctgctct gattctgact
     1201 actgcagtgt tgttctgtct gccggacgca ccttcggctc ccggctgcct ggcggcatcc
     1261 ggtcgtcagg cc
//
LOCUS       DAZFRE010000302         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFRE010000302 DAZFRE010000000
VERSION     DAZFRE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.308"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1247
                     /locus_tag="ACHKUI_005290"
     CDS             <1..>1247
                     /locus_tag="ACHKUI_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO6601260.1"
                     /translation="YGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTL
                     RHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMI
                     NHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHL
                     ARNPEIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKK
                     SKTSYAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDI
                     LNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPL
                     VADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSV
                     LDSGGFAVVFDMGDGYKSLCENMG"
BASE COUNT          293 a          330 c          349 g          275 t
ORIGIN      
        1 tacgggctgc gtgagttctc ctggtcaggt gaacaggctg aacggtttaa cgccattacc
       61 cgggcctatt atatgaaagc ggcagcgaca cagtttccgc tgccggaggg gatgaatctg
      121 cccctgaccc tgcgccatta ccgggtattt atctcgtact gttctccctc gaagaaaaaa
      181 agccgggctg acattctgga aatggaaaac ctggtgaaaa tcatccgggc gtcgttacag
      241 ggggccagta tcgccacaca gacggtggat gcacaggcct ttatcgatat tgtcggggag
      301 atgattaacc ataacccgga ctccctgtac ccgaaaagac gtcagctgga cccgtattct
      361 gatctgaatt atcagtgtgt ggaggacagt tttgacctga atgtccgggc tgattacctg
      421 acgctgggcc tgcgtgagaa cggcaggaac agcacggccc gtatcctgaa tttccatctg
      481 gcccgtaacc cggaaatcgc cttcctgtgg aatatggccg acaactacag caacctgctg
      541 aacccggaac tgtccatctc ctgtccgttc atcctgacgc tgactctggt ggtggaagac
      601 caggtgaaaa cccacagcga agccaacctg aagtacatgg acctggagaa aaagtcgaag
      661 acctcctatg ccaaatggtt tccgtccgtg gagaaagagg cgaaggagtg gggggaactg
      721 cgtcagcgtc tgggctccgg tcagtcttct gtcgtgtcct acttcctcaa catcacagca
      781 ttctgcaagg acaacaatga aacggcgctg gaagtggagc aggacatcct gaacagcttc
      841 cgtaaaaacg gttttgagct gatttcaccg cgctttaacc acatgcgcaa tttcctgacc
      901 tgtctgccct ttatggccgg gaaagggttg tttaaacagc tgaaagaggc cggagtggtt
      961 cagcgcgcag agagctttaa tgttgccaac ctgatgccgt tagtggcgga taaccccctg
     1021 acaccggcag gtctgctggc acccacctac cgtaaccagc tggcgtttat cgatattttc
     1081 ttccggggga tgaataacac caactacaac atggcggtct gtggcacctc cggggccggt
     1141 aaaaccgggc tgatacagcc acttatccgc agcgtgctgg actccggggg ctttgccgtg
     1201 gtgttcgaca tgggggatgg ctacaagtcc ctgtgtgaga acatggg
//
LOCUS       DAZFRE010000303         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFRE010000303 DAZFRE010000000
VERSION     DAZFRE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.309"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1247
                     /locus_tag="ACHKUI_005291"
     CDS             <1..>1247
                     /locus_tag="ACHKUI_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO6601261.1"
                     /translation="YGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTL
                     RHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMI
                     NHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHL
                     ARNPEIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKK
                     SKTSYAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDI
                     LNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPL
                     VADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSV
                     LDSGGFAVVFDMGDGYKSLCENMG"
BASE COUNT          297 a          333 c          343 g          274 t
ORIGIN      
        1 tacgggctgc gtgagttctc ctggtcaggt gaacaggctg aacggtttaa cgccattacc
       61 cgtgcctatt atatgaaagc ggcagcgaca cagttcccgc tgccggaggg gatgaatctg
      121 cccctgaccc tgcgccatta ccgggtgttt atctcgtact gttctccctc gaagaaaaaa
      181 agccgggctg acattctgga aatggaaaat ctggtgaaaa tcatccgtgc ctcttttcac
      241 ggcgcaaaaa tcaccacaca gacagtggat gcacaggctt tcatcgagat tgtcggggag
      301 atgattaacc ataatccgga ctctctgtac ccgaaaagac gtcagctgga cccgtattct
      361 gatctgaatt atcagtgtgt ggaggacagt tttgacctga atgtccgggc tgattacctg
      421 acgctgggcc tgcgtgagaa cggcaggaac agcacggccc gcatcctgaa tttccatctg
      481 gcccgtaacc cggaaatcgc cttcctgtgg aacatggccg acaactacag caatctgctg
      541 aacccggaaa tgtccatttc ctgcccgttc atcctgacgc tgactctggt ggtggaagac
      601 caggtgaaaa cccacagcga agccaacctg aagtacatgg acctggagaa gaagtcgaag
      661 acctcctatg ccaaatggtt tccgtccgtg gagaaagagg cgaaggagtg gggggaactg
      721 cgccagcggc tgggctccgg ccagtcttct gtcgtgtcct acttcctcaa catcacagcc
      781 ttctgcaagg acaataatga aacggcactg gaagtggagc aggacatcct gaacagcttc
      841 cgtaaaaacg gttttgagct gatttcaccg cgctttaacc acatgcgcaa tttcctgacc
      901 tgcctgccct ttatggccgg gaaagggctg tttaaacagc tgaaagaggc cggtgtggtt
      961 cagcgcgcag agagctttaa tgtggccaac ctgatgccgt tagtggcgga taaccccctg
     1021 acaccggcag gtctgctggc gcccacctac cgtaaccagc tggcgtttat cgatatcttc
     1081 tttaagggga tgaataacac caactacaac atggcggtct gtggcacctc cggggccggt
     1141 aaaaccggac tgatacagcc acttatccgc agcgtgctgg actccggggg ctttgccgtg
     1201 gtgttcgaca tgggggatgg ctacaagtcc ctgtgtgaga acatggg
//
LOCUS       DAZFRE010000304         1237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFRE010000304 DAZFRE010000000
VERSION     DAZFRE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.310"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..867
                     /locus_tag="ACHKUI_005292"
     CDS             <1..867
                     /locus_tag="ACHKUI_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6601262.1"
                     /translation="ERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYL
                     TMWRAESLDLNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTE
                     QAREIAIRCHERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRG
                     EWLTIIRVNKSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAK
                     RPPVVNYPGEGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVY
                     ITDMKITEIPQK"
     gene            914..>1237
                     /locus_tag="ACHKUI_005293"
     CDS             914..>1237
                     /locus_tag="ACHKUI_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6601263.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQ"
BASE COUNT          308 a          323 c          377 g          229 t
ORIGIN      
        1 gaacgggcgc ggtcggcact gcttcacgcg aagtataaag aacgcccgga cgttcgctgg
       61 cgtcgtatca aaaaaatcga agctgatttg cgcaaggctg aaaagaccat cgcacagtcg
      121 cagaaatatc tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc
      181 agcagtcatg accatatcag cgcctgtttc ccgctggata cgtatccgcg cccggcagaa
      241 aaaagccagt atgaagggag ccggtcgtta tggtcggccc tggatgatga catcatcacc
      301 acggagcagg cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcagcaa
      361 cgctgggtta accactatca gaaccgcctg atctatgagc gtgccatgct ggacgaaagc
      421 ggcggcgtgg ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc
      481 gagtggctga ctatcatccg cgtgaacaaa agcaacgggg cggtgagttc agtcacaacg
      541 ccaaattaca gttttctcgg gtacagcggc acgatgaaag tgacgcccga tcgcatcacg
      601 gactacaaag caccatcggc agaagaggct gccgtcgcca gtcaggccgc gaagcgtccg
      661 ccggtagtca actatccggg ggaaggtttc cgggaaatga caaaggcgca gtgggccgcc
      721 ctgccccggg actgtaaggc cgtgcgcagt gtggaagaag cagaagacca cggggcatac
      781 cgctaccgcc gcacaatgga caataatttc cgtctggtga atgtgtatat caccgacatg
      841 aaaatcacgg aaatcccaca gaaataaggt atatcccccc gggaaatccc cgggggatga
      901 cggagataat gacatgcaca gtcagttaag agaacgtatc cggctgatgc gcgcaaggct
      961 ggataacgcc gcgccggttg ctgaaatccg ggctgaatct cagctttttg tgagtcctgc
     1021 cccgatctgt gatcgcctgg tgacgctggc ggaaatcagc aaccgtgatc acattctgga
     1081 accctctgcc ggcaccgggg ccatactgcg ggcgattcgg gataccgcgc cggaggccat
     1141 gtgtgatgcg gtggagatta acagcgggct ggtccggtat ctgcgggaaa attttaacgg
     1201 tgtcagggta cagtgtggcg acttcatgga atggcag
//
LOCUS       DAZFRE010000305         1237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFRE010000305 DAZFRE010000000
VERSION     DAZFRE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.311"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..867
                     /locus_tag="ACHKUI_005294"
     CDS             <1..867
                     /locus_tag="ACHKUI_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6601264.1"
                     /translation="ERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYL
                     TMWRAESLDLNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTE
                     QAREIAIRCHERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRG
                     EWLTIIRVNKSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAK
                     RPPVVNYPGEGFREMTKTQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVY
                     ITDIKITEIPQK"
     gene            914..>1237
                     /locus_tag="ACHKUI_005295"
     CDS             914..>1237
                     /locus_tag="ACHKUI_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6601265.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQ"
BASE COUNT          306 a          325 c          376 g          230 t
ORIGIN      
        1 gagcgggcgc ggtcggcact gcttcacgcg aagtataaag aacgcccgga cgttcgctgg
       61 cgtcgtatca aaaaaatcga agcggatttg cgcaaggctg aaaagaccat cgcgcagtcg
      121 cagaaatatc tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc
      181 agcagtcatg accatatcag cgcctgtttc ccgctggata cgtatccgcg cccggcagaa
      241 aaaagccagt atgaaggaag tcggtcgtta tggtcggccc tggatgatga catcatcacc
      301 acggagcagg cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcagcaa
      361 cgctgggtta accactatca gaaccgcctg atctatgagc gtgccatgct ggacgaaagc
      421 ggcggcgtgg ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc
      481 gagtggctga ccatcatccg cgtgaacaaa agcaacgggg cggtgagttc agtcacaacg
      541 ccgaattaca gttttctcgg gtacagcggc acgatgaaag tgacacccga tcgcatcacg
      601 gactacaaag caccatcggc agaagaggct gccgtcgcca gtcaggccgc gaagcgtccg
      661 ccggtagtca actatccggg ggaaggtttc cgggaaatga caaagacgca gtgggccgcc
      721 ctgccccggg actgtaaggc cgtgcgcagt gtggcagaga cagaagacca cggggcatac
      781 cgctaccgcc gcacaatgga caataatttc cgcctggtga atgtgtatat taccgacata
      841 aaaatcacgg aaatcccaca gaaataaggt atatcccccc gggaaatccc cgggggatga
      901 cggagataat gacatgcaca gtcagttaag agaacgtatc cggctgatgc gcgcaaggct
      961 ggataacgcc gcgccggttg ctgaaatccg ggctgaatct cagctttttg tgactcctgc
     1021 cccggtctgt gatcgcctgg tgacgctggc ggaaatcagc aaccgtgatc acattctgga
     1081 accctctgcc ggcaccgggg ccatactgcg ggctattcgg gataccgcgc cggaggccat
     1141 gtgtgatgcg gtggagatta acagcgggct ggtccggtat ctgcgggaaa attttaacgg
     1201 tgtcagggtt cagtgtggcg acttcatgga atggcag
//
LOCUS       DAZFRE010000306         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFRE010000306 DAZFRE010000000
VERSION     DAZFRE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.312"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..482
                     /locus_tag="ACHKUI_005296"
     CDS             <1..482
                     /locus_tag="ACHKUI_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601266.1"
                     /translation="EDNNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYREN
                     SLMNSCRTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSKNRTYHKTRYLWNVL
                     LNGDFELLNQLVESNEKELKDCNLLGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            528..>1235
                     /locus_tag="ACHKUI_005297"
     CDS             528..>1235
                     /locus_tag="ACHKUI_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFO6601267.1"
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRIN"
BASE COUNT          400 a          197 c          209 g          429 t
ORIGIN      
        1 tggaagataa taatcttttt tttgatgaag atttacgact aggagaagat ctggatttta
       61 tctatcgact gctaattaca tgcgatatgt atgcggttcc atattatatg tataagcata
      121 actatagaga aaattcctta atgaactcat gtagaaccat cactcattat cgacatgagt
      181 catttgcgca cgaaagaatc tactcttctg tgatgcagtt atacaaaggt aaccggaaag
      241 aagaaattca tacattattg agtaaaaata gaacttatca taaaactcgt tatttatgga
      301 atgttctact taatggcgat tttgaattat taaatcaatt agttgaaagc aatgaaaaag
      361 aattgaaaga ttgtaatctt cttggcaaga gagataagag gcgagcaaag atattagcat
      421 caaaaaacta tattctctgg aggatggtaa gactggtaaa tagaaaaaag aataaacgtt
      481 aggtcttgca ttaaataacc catcctcgta gcataggttg aaattatatg tcgttttgtt
      541 ggaatgaaat taactctggt gtcaagtctt taattctcat attatgtatg ttgtctttaa
      601 tgactttgtc tttgtgggat gatgttgcaa caaagtttct tcatgcagct ggaattatat
      661 ctgcattata ttttctagcg acaccaaaaa aaacaataac taataatcct actttgttaa
      721 ttttcatctc attatgtctt ttgggtatcg ttaatatcat ctggtattca cattataaag
      781 tttcaggctc ggtttatacc aatgcatatc gtggcccaat ggaaactgga aaaattgcct
      841 tgtgtagtgc ttttattttc ttagttcttt ttgctaaaga tgagatgaga accaaaataa
      901 aatttgggaa actaattctg ttcgcatcct tggcaacgca gttacttttt tttgcgcatg
      961 ccatgtggca acatttctat ttaaacgtcg accgtgttgc attatcagct tcccacgcta
     1021 caacagcagg ctacatcatc cttttccctt ctttactggc atcaattctc attttaaaat
     1081 ccgactttag acataaaaca acattatata caattaactt catgcttagc ttatgtgctg
     1141 ttatagtaac tgaaacgcgt gcagccatat tagtgtttcc attctttgcg ttactattaa
     1201 tcgtaatgga tagttatatt aataagcgaa ttaat
//
LOCUS       DAZFRE010000307         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFRE010000307 DAZFRE010000000
VERSION     DAZFRE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.313"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(139..832)
                     /locus_tag="ACHKUI_005298"
                     /pseudo
     CDS             complement(139..832)
                     /locus_tag="ACHKUI_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          371 a          272 c          253 g          336 t
ORIGIN      
        1 aatcattaga tacccccaga tttagttgta aagcaagcac actttgcatt tcgagacaat
       61 ccaggtaaag actcttacca cccattttgt gatttttata cttatgatga taagttgaac
      121 gttgggtagt gcttccaatc agtagaacat gtgcttttca atgaaggaac cgatgacttt
      181 ttcgtggatt tcaacggaac gcgagaagca gattgtttta cttaccagcc gcttgatgcg
      241 ggtgcggagg gtcaggttgt tacgttcaat acgttgcgtg aagattttgc cggtcaggta
      301 tttcaccttc ggcacttctc tggcgtagct gccccagtca tcgcggccac catgccgata
      361 cagaacggag tcaacagggc taacaattcc cgacaggcct catcattccg tggcccgaaa
      421 gtgtaagcca acacaccgcc agttttagtg ttctacgcat accagagcca gtgttgttgc
      481 accttgttgc caacgaagct ccacagctca tcaagttcac agataagggc aacatcagcg
      541 tgagctgact gacgcagatg ttatccgctg gggcgagagc tttttaatgt ccgtatgacg
      601 gtattgatgc tgattttcag tgttctggcg atatcacggc ccctgtgcca ttatgcgcca
      661 tttcaacgat cagctcctta aagccgggtt ttctggcttc ataagagtaa gtgagctgga
      721 aaacacgttt acatgaacga caacggaaac gctcatgctg ggaacaacta cgaccatgtc
      781 gataaacttt atcggaatta cagcgcggac aatgaacagt aacggtagcc atggaaaacc
      841 ttaaatacca gtagattacc agaaacttct atcaattgaa gacattaccg tttttgatta
      901 cagcaaatgt tttattaatt accccaccat cgactaaatc cgttagaacg tcgaatgatg
      961 ccattcgtaa atacattaaa aagactgttg taatcaacga agatggaaaa cagtgtgtta
     1021 aaactaatct gaacttcaga aaaacagatg atggtatggt gatatatata tccgctctga
     1081 accgccacgc aatttaaaat aaaaatcaac aaatatcgtc gatatccata tataccattt
     1141 ttctgtagtt gtattagcgt cgcatatttt atgactttaa gtcataagtc aaaacacctg
     1201 agaaaaagca aacaacattt tcattaccac ca
//
LOCUS       DAZFRE010000308         1228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFRE010000308 DAZFRE010000000
VERSION     DAZFRE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.314"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(210..>1228)
                     /gene="kfiD"
                     /locus_tag="ACHKUI_005299"
     CDS             complement(210..>1228)
                     /gene="kfiD"
                     /locus_tag="ACHKUI_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis UDP-glucose
                     dehydrogenase KfiD"
                     /protein_id="HFO6601268.1"
                     /translation="ENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDT
                     SSVEAVIRDVTEINPNAIMVVKSTVPVGFTKTIKEHLGINNIIFSPEFLREGRALYDN
                     LHPSRIIIGECSERAERLAVLFQEGAIKQNIPVLFTDSTEAEAIKLFSNTYLAMRVAF
                     FNELDSYAESFGLNTRQIIDGVCLDPRIGNYYNNPSFGYGGYCLPKDTKQLLANYQSV
                     PNKLISAIVDANRTRKDFITNVILKHRPQVVGVYRLIMKSGSDNFRDSSILGIIKRIK
                     KKGVKVIIYEPLISGDTFFNSPLERELAIFKGKADIIITNRMSEELNDVVDKVYSRDL
                     FKCD"
BASE COUNT          421 a          230 c          193 g          384 t
ORIGIN      
        1 aatatacatt tccaacgcac aagccaagta ttctaattaa cattagagtt atgaaaaatt
       61 cgatataaac acaacagcag ggcaagaaat ttaaccaaca tcttatttta cgatcgagga
      121 ttaataaaga acataccaac gaggcatatt tttaacggat taaataatgc atttccttct
      181 cttaatagtt aatagtatat aacaatacat tagtcacatt taaacaaatc gcgactatag
      241 actttgtcga ccacatcgtt caactcctct gacattcggt tagtgataat aatatcagct
      301 ttccctttaa agatcgccag ctcccgttcc aaaggtgagt taaagaatgt atctccagaa
      361 ataagcggct cataaataat tactttcacg ccttttttct tgatacgctt tataatacca
      421 agaatagaag aatctctaaa attatctgaa ccacttttca taatcaaacg ataaaccccc
      481 acaacttgtg gtctatgttt caaaataaca ttagtgataa agtccttacg tgtacggtta
      541 gcatcaacaa ttgcagatat aagtttattc ggaacagact gatagttggc taataattgc
      601 ttggtatctt ttggcaaaca gtagccacca taaccaaaag aaggattatt gtagtaatta
      661 ccaatgcgcg gatccaaaca aacaccgtca ataatctgac gcgtattcag accaaaactt
      721 tctgcgtaac tatccaattc attaaaaaat gcaactcgca tagccaaata agtatttgaa
      781 aataacttaa tcgcttccgc ttccgtagaa tctgtaaata aaacgggtat attttgttta
      841 atcgctcctt cctgaaataa cactgccaaa cgttctgccc gttcagaaca ttcaccgata
      901 ataatgcgag atggatggag attatcgtat agggctcttc cttctcgtaa aaattctgga
      961 gagaagataa tattattaat acctaaatgt tctttaattg tttttgtgaa acctactggg
     1021 accgtagatt taaccaccat aattgcgttt gggttgattt ccgttacgtc acgaatgaca
     1081 gcttcaacgc ttgatgtatc aaagtaattt gaacctgggt cataattcgt tggtgtagca
     1141 ataataacgt aattggcatt tttataggct tcatatttgt tagtagttgc gcgaaaatta
     1201 agtattttag ttgaaagata attttcaa
//
LOCUS       DAZFRE010000309         1227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFRE010000309 DAZFRE010000000
VERSION     DAZFRE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.315"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..273
                     /locus_tag="ACHKUI_005300"
     CDS             <1..273
                     /locus_tag="ACHKUI_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601269.1"
                     /translation="DNFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCG
                     WQLYERGELVDVLWGELEWSSPTDDDELPEVTGPAWIVDNVTHYGG"
     gene            270..581
                     /locus_tag="ACHKUI_005301"
     CDS             270..581
                     /locus_tag="ACHKUI_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601270.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARGGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            658..>1227
                     /locus_tag="ACHKUI_005302"
     CDS             658..>1227
                     /locus_tag="ACHKUI_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6601271.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIG"
BASE COUNT          236 a          356 c          373 g          262 t
ORIGIN      
        1 gacaacttca ttcaggtgga tttcgacacg ccgtggtgtc agccggaaag cgacgttgtt
       61 gcagaattaa gccgccgttt cggctgcacg ctggagcact ggtacgccga acagggctgc
      121 aatttctgtg gctggcagtt gtatgagcgc ggagagcttg ttgatgtgct gtggggggaa
      181 ctggaatggt cttccccgac agatgacgat gagctgccgg aagtcaccgg acctgcgtgg
      241 atagtcgaca atgtgacgca ttatggcgga tgaagtatga cggagacggg cgggcaaccg
      301 cccgtttctt ttccgacaaa ggatgtcgcc gtgctcctct ttttactgcg ccggctgacg
      361 cggcgcggca taaacgctgc ctgtggtcag tgtcccgcgt ccggcgggca cggggcgggc
      421 gcttttaccg ctcccggctg gcgtcggtga cagtgtacgc cagcccgtcg cccttttctg
      481 atgaacgccc ttcaagccgc tttcgcggca taaccttgcc gtcaaaaaga cgacggctgc
      541 ggtattccac ggtcggcctg acccgttacc ggacgcggtg aacagcccac aggcagcggg
      601 gaacgggcac cacaggggtg ccctcccggt gtcctctgta agagaaggag tgctgttatg
      661 tcccgtttta tcctgggtga ctgcgtgcgt gttatggcca cgtttccggg caatgctgtc
      721 gatttcatcc tcaccgaccc gccgtatctc gtcggtttcc gtgaccgttc cgggcgcacc
      781 atcgccggcg ataaaaccga tgaatggctg caaccggcct gtaatgaaat gtaccgcgtg
      841 ctgaaaaaag atgcgctgat ggtcagcttc tacggctgga accgcgtcga tcgctttatg
      901 gccgcctgga aaaatgcggg attcagcgtg gttggccacc tggtgtttac caaaacctac
      961 acatcgaagg ccgcatatgt gggctatcgc cacgaatgcg cctacatcct ggcaaaagga
     1021 cgtccggcgc tgcctcagaa gccattgccc gacgtgctgg gctggaaata ttcaggcaat
     1081 cgtcatcacc cgactgaaaa gcccgttacc agcctgcaac cgctcattga gagcttcaca
     1141 cacccgaacg cgattgtgct ggacccgttt gcaggcagcg gctcaacctg tgtggccgcc
     1201 ctccagtccg gacgccggta tatcggt
//
LOCUS       DAZFRE010000310         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFRE010000310 DAZFRE010000000
VERSION     DAZFRE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.316"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..1181)
                     /locus_tag="ACHKUI_005303"
     CDS             complement(<1..1181)
                     /locus_tag="ACHKUI_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO6601272.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     E"
BASE COUNT          264 a          364 c          286 g          307 t
ORIGIN      
        1 tactcatgca gacaggttcc ctcatcggaa taaaacgcgc actgacgtga ggccagcgta
       61 caggccggat tatcgacata tttctgacag gtgccgttat ctgcggactg agtgacatat
      121 ttatcccggt atgcccagca tgcctgtgtg atgttccagg gtttaccttc catcactccg
      181 gttttcgtac cacccggctc tgaacactca gtgcctgtca gcacgccatc cgctttatta
      241 aacggacaac tctccaccca ctccacccgg ggaacccatt ctttatcacg gaccttcatc
      301 ctgagtttca gcgtaaaggt ggaagcacca ctgacaagcg attcatagac tttcctgtca
      361 ccattcccgt gcgggaggca attaccgtta gcagtacagc cactaccggt cagaacctgc
      421 ccctgtgtca cctgcaaacc tgtcgccccg gacagggggt aagtatccga ctgcccgttt
      481 gccaggccga cattaaaggt tgtattcatg aaggtataac gggagttcag gaagaaaaaa
      541 gcggcgtata cagacaatga tgcactttcc actgtaccgg ttaccggagc cgtgacactg
      601 aacaccagtt tcagaccgtt catactgaac cggaactgtg agtgcggtat cgtcacctca
      661 cgatattcat ccgtgtattt ccagtccccg gtgatactgg cagtccgtgt gcagtactcc
      721 tccaccatcg tgtcccgctc acaggtatag tttgtgaact cactgcggtt tatctgctgc
      781 gcactgcact gctgcccggt gtttcccaca atgctgtcag cccggttcac cacatccctg
      841 ccggtctgaa tgaacggtgc atccggtgac agaatgtctt tcggcttgtt catgaaggac
      901 tctgtgatgg tttttccggt ttcaccggtc gcccattccg tggtgccgtc atttttcagg
      961 ccgccatccc cgccggcagt cacgccaccg taatattttg tctcgtccgg attcgcgtta
     1021 tagctgggga tactctcctg tggcttaaag ccctgaatac tgcctgtacc ctgcccctga
     1081 atctgacggg caaaatcaga accggcccgg taatcactgt tgctgtcagc ctgtgccatg
     1141 ccggcaacca gagccagtat cagaggtaaa atacgtttca tttcccggaa tctcctttcc
     1201 ctgccagtaa atcatgagcc a
//
LOCUS       DAZFRE010000311         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFRE010000311 DAZFRE010000000
VERSION     DAZFRE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.317"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            66..>1213
                     /locus_tag="ACHKUI_005304"
     rRNA            66..>1213
                     /locus_tag="ACHKUI_005304"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          343 a          261 c          369 g          240 t
ORIGIN      
        1 gttcgtgagt ctctcaaatt ttcgcaacac gatgatggat cgcaagaaac atcttcgggt
       61 tgtgaggtta agcgactaag cgtacacggt ggatgccctg gcagtcagag gcgatgaagg
      121 acgtgctaat ctgcgataag cgtcggtgag gtgatatgaa ccgttataac cggcgatttc
      181 cgaatgggga aacccagtgt gtttcgacac actatcatta actgaatcca taggttaatg
      241 aggcgaaccg ggggaactga aacatctaag taccccgagg aaaagaaatc aaccgagatt
      301 cccccagtag cggcgagcga acggggagga gcccagagcc tgaatcagtg tgtgtgttag
      361 tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa aaatgcacat
      421 attgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat ggggggacca
      481 tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg agggaaaggc
      541 gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt acaagcagtg
      601 ggagcctctt aatggggtga ctgcgtacct tttgtataat gggtcagcga cttatattct
      661 gtagcaaggt taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag
      721 ttgcagggta tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa
      781 cactaactgg aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg
      841 ggtgaaaggc caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg
      901 cctcgtgaat tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta
      961 ccaacccgat gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg
     1021 ctaacgtccg tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg
     1081 gttaagtggg aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc
     1141 atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg
     1201 gggctaaacc atg
//
LOCUS       DAZFRE010000312         1199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFRE010000312 DAZFRE010000000
VERSION     DAZFRE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.318"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..855
                     /locus_tag="ACHKUI_005305"
     CDS             <1..855
                     /locus_tag="ACHKUI_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6601273.1"
                     /translation="AFLSAIHHQIFENQLDTETITVSYEFILCQHLITNTEFSYLALP
                     ENYNRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKG
                     YDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRCQQKLLDSIDIIEIIYI
                     KRPSKDLANLRKEFNKNVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHK
                     LSLDDGGTNDLENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPM
                     KNIMEPTK"
     gene            852..>1199
                     /gene="yrhA"
                     /locus_tag="ACHKUI_005306"
     CDS             852..>1199
                     /gene="yrhA"
                     /locus_tag="ACHKUI_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFO6601274.1"
                     /translation="MMTKTQISKLKKMMNDLDYPFEAPLKESFIESIIQIKFNSNSTN
                     CLEKLCNEVSILFNNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRD
                     NDDFINPDLQERLV"
BASE COUNT          454 a          203 c          195 g          347 t
ORIGIN      
        1 gcttttttat cggctattca tcaccagatc tttgaaaacc aactggatac agaaacaata
       61 actgttagtt atgaatttat cctctgccaa catcttatca ctaacaccga gttcagctat
      121 ttggcactcc ctgaaaatta taaccgcttg tttttaccaa attcaaaaaa caaaacaaat
      181 aatggtttaa aaacgttaaa cagcgaagct gttggcaggc tacttgctgc tggtggcgta
      241 tacaatggga acattgaagg attcagagat actgcggaaa aactgggtgg agatgcaatc
      301 aaaggctatg atcaaatact aaatgaaaaa acagcgggca tagcgatagc aacagcatct
      361 attcttttaa caaagcgttc taatgttgat acatatacag aaataaatag ttacttaggc
      421 aaacttagat gccaacaaaa acttcttgat agtatagaca taatagaaat aatatacatt
      481 aagagacctt caaaagactt agctaactta cgaaaggagt ttaataaaaa tgtaagaaaa
      541 aattttctta tcaaacttgc aaaaacctcc gaagcatctg gaagattcaa cgccgaagac
      601 cttttaagaa tgagaaaggg caatgttcct ctaaattata atgtacacca taaactatct
      661 ctagatgatg gtggtactaa tgatttggaa aatttagtat taatcgaaaa cgaaccatat
      721 cataaggttt ttactaacat gcaatcacga atagctaagg gaatattagt gggtgaaagc
      781 aaaatcactc cctgggccat tccatctggc tcaatttatc ctcccatgaa aaatattatg
      841 gaacccacaa aatgatgact aaaacccaaa taagtaaatt aaaaaaaatg atgaatgatt
      901 tagactatcc atttgaagca ccgctcaagg aatcatttat tgaaagtata atccaaataa
      961 aatttaattc taactcaact aattgcctgg agaagttatg taatgaagtt agtattctct
     1021 ttaataatca acctgattat cttacttttt taagagcaat ggatggattc gaagttaatg
     1081 gattacgatt atttagcctc tcgattccag aaccttcagt taaaaacctt tttgccgtaa
     1141 atgaatttta tagagataat gatgatttca taaatcctga cttacaagaa cggttagtg
//
LOCUS       DAZFRE010000313         1194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFRE010000313 DAZFRE010000000
VERSION     DAZFRE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.319"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..386
                     /locus_tag="ACHKUI_005307"
     CDS             <1..386
                     /locus_tag="ACHKUI_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6601275.1"
                     /translation="KESRYRVNAIGINPVTGYGSGLMQVDSQHFNELDRYGIKPEHLT
                     TDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLAYASEVYRIYTG
                     IKSSKGIRIPVMKKSLPEINSVQNN"
     gene            complement(682..>1194)
                     /locus_tag="ACHKUI_005308"
     CDS             complement(682..>1194)
                     /locus_tag="ACHKUI_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601276.1"
                     /translation="NSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQ
                     VIEGAYEVLGVFDKVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQII
                     TPRRREDKQNDLWTTCQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWV
                     IAEQFRKWKS"
BASE COUNT          341 a          293 c          253 g          307 t
ORIGIN      
        1 ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa tccggtaacg ggatatggca
       61 gcggactgat gcaggtagat tcccagcatt ttaacgaact ggaccgctat ggaattaaac
      121 cggaacatct gacaacagat ccctgcatga acatttatac cggtgcttat tatctggcaa
      181 tagcctttaa aaaatggggc gtctcctggg aggccgttgg tgcatacaat gccggattca
      241 ggaagaccga acgccagaac cagagacgtc ttgcctacgc atcagaggtt taccggattt
      301 ataccgggat aaaaagcagt aaaggtatcc ggattccggt catgaagaaa tcacttccgg
      361 aaattaacag cgtgcagaac aattaatctt acagacattg tcagataccg gttatgccgc
      421 aaaagcggca tggaaggcca cacacaaaaa tatcatatga gtgacaatgc tgactggccg
      481 aagccccgcg aagcgggaac ccatcttata tatacccggt aactgcgcag cctcctgcca
      541 gaccattttt cgcgggcaca atatttacag acaaccggaa agccgattga tggaattgag
      601 gtatatcaga cgacacagcg atgccgaaga atgataatat aagtaaagcc ccgaaaaatt
      661 ttcggggctt tacttatatt atcatgactt ccactttctg aactgttcgg caatcaccca
      721 taacgccttg ttgattcgga tatcaccatc aatacctgta atggctcttg tcctggtatt
      781 tttaccggag gcacttcgcc ccgacaatcc accttttatc atattctcct gaacccgctg
      841 acaggttgtc cagagatcgt tctgtttatc ttcccgacga cggggagtaa ttatttgttc
      901 aggcgtcact ggcgttttat tttcatcttc atacctgacc atcagtgcag ctctgccaaa
      961 taaatgttgc tcgtcactgt taagatgaat ttctttcatc gcctccatat tatcagtgac
     1021 cttatcaaag acaccgagca cttcatacgc tccctcgata acctgcccga caatatcacc
     1081 tttatgtgga acgcggattt cgccaaaatt atttccgcac accaggccat ttgtgcagac
     1141 aaaacgaaaa attccgggga tcatctgata actggatgaa ccatcatgtg aatt
//
LOCUS       DAZFRE010000314         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFRE010000314 DAZFRE010000000
VERSION     DAZFRE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.320"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1176
                     /locus_tag="ACHKUI_005309"
     CDS             <1..>1176
                     /locus_tag="ACHKUI_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFO6601277.1"
                     /translation="TPQETALISPIRELTYRQLSTAADHVARALLALGVQHGDRVAVV
                     MEKGWQQIAAVHGILRLGAVYLPVDPVLPPQRRQLLLTVGEVRVQVTQPGLTQLEPSL
                     PVLIIDDGMLDTPAAPLPEVAGDVTDLAYIIFTSGSTGTPKGVMIDHRAAMNTLEDIN
                     ERFGLNAQDRVFGLSSLSFDLSVYDAFAPFMVGAALVLPEAGREKDPRHWQTVMAHGH
                     VSVWNAVPALMQMLCEYHSGDRMSYPTLRLALLSGDWIPLTLPEQMRERLNETMDIIS
                     LGGATECAIWSVYYPIGEVESTWTSIPYGRGLRNQPVYVLNAQLEECPVGVEGEICIG
                     GMGLAQGYLNDAEKTAASFVWREASGERIYRTGDRGRYFADGQVAFLGRNDTQVKVNG
                     "
BASE COUNT          240 a          303 c          391 g          242 t
ORIGIN      
        1 acgccacagg aaactgcgct gatcagtcct atccgtgaat tgacctatcg ccaactgtcg
       61 acggcggcgg atcatgtggc ccgcgccctg ttagcgctgg gcgtgcagca tggcgaccgc
      121 gtggcggtgg tgatggaaaa aggctggcag cagattgccg ccgtacacgg cattttacga
      181 ctgggtgcgg tctatctgcc agtggatccg gtgctaccgc cacagcgtcg ccagcttttg
      241 ctgacggtgg gcgaggtgcg ggtacaagta acgcagccgg gtctcacgca attggagccg
      301 tcgctgcccg tgctgatcat cgacgacgga atgctggaca cgcctgctgc gccgttgcct
      361 gaagtggctg gggatgtcac ggatctggcc tatatcattt tcacttccgg ctccaccggt
      421 accccgaaag gagtgatgat cgaccaccgt gcggccatga acacgctgga agacatcaac
      481 gaacgctttg gcctcaatgc gcaggatagg gtgttcgggc tgtcatcatt gagctttgac
      541 ctgtcggttt acgatgcctt tgcgcctttt atggtgggtg cagcgctggt actgccggaa
      601 gcaggacggg aaaaagatcc gcgtcattgg cagacagtta tggcacacgg tcatgtaagc
      661 gtctggaatg cagtgcccgc actgatgcag atgctgtgcg aataccacag cggcgatcgg
      721 atgagttatc cgacgttgcg tctggcactg ttgagcggcg actggatccc gctaacgtta
      781 ccggagcaga tgcgcgagcg gctcaatgaa acgatggaca tcatcagtct gggtggagcg
      841 accgagtgcg ccatctggtc ggtctactac ccgataggtg aggtggaatc gacgtggacc
      901 agtattccct acggtcgggg cctgcgcaac cagccagtat acgtgctaaa tgcgcaactg
      961 gaggaatgtc cggtcggggt ggaaggagag atttgcattg gcgggatggg gctggcacaa
     1021 ggctacctga acgacgcaga gaaaacggcg gcgagctttg tctggcgcga agcgagtggt
     1081 gagcgaattt accgcactgg ggatcgcggg cgctactttg ctgacgggca agtcgccttt
     1141 ttggggcgca acgataccca agtgaaggtg aatggt
//
LOCUS       DAZFRE010000315         1167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFRE010000315 DAZFRE010000000
VERSION     DAZFRE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.321"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1155
                     /locus_tag="ACHKUI_005310"
     rRNA            <1..1155
                     /locus_tag="ACHKUI_005310"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          284 a          264 c          363 g          256 t
ORIGIN      
        1 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
       61 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggttagcggt
      121 aacgcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag
      181 gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg
      241 ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg
      301 caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat
      361 gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga
      421 aataccaccc tttaatgttt gatgttctaa cgttggcccg taatccgggt tgcggacagt
      481 gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag
      541 gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg
      601 cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg
      661 gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa
      721 gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg
      781 aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga
      841 acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc
      901 tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg
      961 cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt
     1021 gccccgagat gagttctccc tgagacttag agtctcctga aggaacgttg aagacgacga
     1081 cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc
     1141 gtgaggctta accttacaac gccgaag
//
LOCUS       DAZFRE010000316         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFRE010000316 DAZFRE010000000
VERSION     DAZFRE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.322"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            2..1039
                     /gene="ompC"
                     /locus_tag="ACHKUI_005311"
     CDS             2..1039
                     /gene="ompC"
                     /locus_tag="ACHKUI_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6601278.1"
                     /translation="MMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYA
                     RLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGR
                     NYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQY
                     QGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQVAYGKSKFNASGKNA
                     EVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEAVAQYQFDFGLRPSVA
                     YLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINLIDDSKFTKTAGIDTD
                     DIVAVGLVYQF"
BASE COUNT          322 a          246 c          256 g          313 t
ORIGIN      
        1 actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta acaagctgga
       61 tctatacggg aaagttaatg ccaagcacta cttctcctct aatgatgcag atgatggtga
      121 tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc aactgactgg
      181 tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa
      241 agacaaaacc cgtcttgcat ttgcaggcct gaaatttggt gattacggct caatcgatta
      301 cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc tgccagaatt
      361 cggtggtgat acctggaccc aaacagatgt gttcatgact ggtcgcacta ctggtgttgc
      421 aacttatcgt aacaacgact tctttggtct ggtcgatggc ctgaactttg ctgctcagta
      481 tcagggtaaa aatgaccgca ctgacgtaac tgaagccaat ggtgatggtt tcggtttctc
      541 cactacttat gagtatgaag gattcggcgt gggtgcaacc tatgctaaat cagatcgcac
      601 tgacggtcag gtcgcctatg gtaagagcaa attcaatgcc tccggcaaaa atgcggaagt
      661 atgggctgca ggcctgaaat atgatgcgaa caatatctat ctggctacca catattctga
      721 aactcagaat atgaccgttt ttggtaataa ccatattgca aacaaagcac aaaactttga
      781 agcagtagca caatatcagt ttgacttcgg tctgcgacca tctgttgctt accttcagtc
      841 aaaaggtaaa gaccttggtg ttcatggtga ccgagactta gtcaagtatg tcgatgtcgg
      901 tgctacttac tactttaata aaaacatgtc cacttttgtt gattacaaaa tcaacttaat
      961 tgacgatagt aagtttacca aaacagctgg tattgatacc gacgacatcg tcgctgtagg
     1021 tctggtttat cagttctaat ctgacttacg aaaaagatat gttgcgggag gctttgcctc
     1081 cgcaacatat aagtggagcc ctcaagccac ttcctttaga agcactacct tgcttct
//
LOCUS       DAZFRE010000317         1136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFRE010000317 DAZFRE010000000
VERSION     DAZFRE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.323"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            90..1109
                     /locus_tag="ACHKUI_005312"
     CDS             90..1109
                     /locus_tag="ACHKUI_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6601279.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT          429 a          204 c          206 g          297 t
ORIGIN      
        1 aaatcacaac aaaaacattt gataatcact tagtgacaaa ataaaattga gacgattttg
       61 ttataagaga ggcgacatgg agtgagataa tgaataacga acagatgctt atcaaatgca
      121 aagatccccg taaatgtcgg ttttatgccg aatatctgaa tcacaaatac cccaatgaat
      181 tccctcagtt tgcgcctgac aacccacaaa actgtatcag aaatgttaat tcgtttttaa
      241 aagaaaagta ttataaagct tcaaacaatt cttttggtgt ttttggttcg actgcagaag
      301 aagctctcag caaagaaatt aatgaacttg aaattattgc agggcaggca atactgcctg
      361 aagagagttt tctctggttg aaaactgacg aacttgcgac tttctttacc tggactacta
      421 tttatctgtc gaaggaagac catacaaaat tcccgaaaat aaaccataat tcatcaggaa
      481 taaaaaacgg aattgaagtc aggaaaatac gaacagttac cgatgaaacg ttaaatactt
      541 acaaaaaaag taaccttccg gataatccat catcccataa cgaacgaata aaagtaatca
      601 tttcatattt tgactcctgc cattctttaa ttaataaaaa tgcaaaaagc atatacctcg
      661 aggaactcag acaggcgtgg cttaaaaaga aaagcgcttg tcaaaagttt aagtggctcc
      721 ttcctgacga tgatgaaatg tgtcagtggt tctggacaag actgaaacaa aaacaggatg
      781 aaaaacatcc agtacataac ccagtgaccc ggtggttcat accatcttca acgaaagaat
      841 attatcttgc ctctcttgtc gcctttgctt tatggaaaga agcgcctgat accattgaac
      901 tgttcagaac acgaataaat catgcctggc atcagaaaaa acaaaaggac aaatatcgga
      961 gtgatggtaa aaaggccatt aatatacata taagaagcaa tgtgaaagaa atgcttgatg
     1021 agctcagaac tgtttacggt ctgagaacag gagagttttt tgaaatgctg attacagaga
     1081 aatatcttga acacaagcga aggaagtaat tcattacctg ttacattcta aataat
//
LOCUS       DAZFRE010000318         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFRE010000318 DAZFRE010000000
VERSION     DAZFRE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.324"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1132
                     /locus_tag="ACHKUI_005313"
     CDS             <1..>1132
                     /locus_tag="ACHKUI_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HFO6601280.1"
                     /translation="DSTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEIAGNNAV
                     VNQDGELDVSGGGHGIDITGDSATVDNKGGMTVADADSIGIQIDGDKAVVNNDGDSAI
                     SNGGTGTQVNGDEATVNNNGNTTVDGKDSTGTEINGDKAIVNNDGDSTILDGGTGTRI
                     TGDDATANNSGNTTVDGQGSTGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATVDN
                     KGGMTVADADSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNNGNTTVDGK
                     DSTGTEINGDKAIVNNDGDSTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEIAG
                     NNSVVNQDGELDVSGGGHGIDITGDSATVDNKGGMTVADADSI"
BASE COUNT          305 a          293 c          367 g          167 t
ORIGIN      
        1 gacagcacga tccttgatgg cggcaccgga acccgcatta cgggtgatga tgcgacggcc
       61 aacaacagcg gcaataccac cgtagacggt cagggttcga ccggaactga aatcgcgggt
      121 aacaacgcgg tggtgaacca ggacggcgag ctggatgtga gcggtggcgg tcacgggatt
      181 gatatcaccg gcgacagcgc aacggtggac aacaaaggcg gcatgaccgt tgcggatgcg
      241 gactccatcg gtattcagat agacggcgac aaggcggtcg tgaacaacga cggtgacagt
      301 gccatcagca acggcggcac cggcacgcag gttaatggcg acgaagccac cgtgaacaat
      361 aacggcaaca ccaccgttga cggtaaggat tccacgggca ccgagataaa cggtgacaaa
      421 gctatcgtca acaacgacgg tgacagcacg atccttgatg gcggcaccgg aacccgcatt
      481 acgggtgatg atgcgacggc caacaacagt ggcaatacca ccgtagacgg tcagggttcg
      541 accggaactg aaatcgcggg taataacgcg gtggtgaatc aggacggcga gctggatgtg
      601 agcggtggcg gtcacggaat tgatatcacc ggcgacagcg caacggtgga caacaaaggc
      661 ggcatgaccg ttgcggatgc ggactctatc ggtattcaga tcgacggcga caaggcggtt
      721 gtcaataacg acggtgacaa cgccatcagc aacggcggca ccggcacgca ggttaacggt
      781 gatgaagcca ccgtaaacaa taacggcaac accaccgttg acggcaagga ttccaccggc
      841 accgagataa acggtgacaa agctatcgtc aacaacgacg gcgacagcac gatccttgat
      901 ggcggcaccg gaacccgcat tacgggtgat gatgcgacgg ccaacaacag cggcaatacc
      961 accgttgatg gtcagggttc gaccggaact gaaatcgcgg gtaataactc ggtggtgaat
     1021 caggacggcg agctggatgt gagcggcggc ggtcacggta ttgatatcac cggcgacagc
     1081 gcaacggtgg ataacaaagg cggtatgacc gtcgcggatg cggattcgat tg
//
LOCUS       DAZFRE010000319         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFRE010000319 DAZFRE010000000
VERSION     DAZFRE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.325"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            378..941
                     /gene="ipbA"
                     /locus_tag="ACHKUI_005314"
                     /gene_synonym="fimX"
     CDS             378..941
                     /gene="ipbA"
                     /locus_tag="ACHKUI_005314"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO6601281.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          374 a          214 c          188 g          331 t
ORIGIN      
        1 cctccaaatt tatgcatcaa aaaaaacaaa agtaaaatca attagataca caacaaggca
       61 atctcacagg aaatgatatt aaatatctca aaactaataa aaaattacta aaacattaga
      121 cgtaacacac atcccacacg acctgtcact catagtttta atatttattt tttcacatta
      181 tagtagataa aaacacgcat taaaaatgac ttgtagaaaa gctgctcaat aaatagttca
      241 tatttaatct gttgcgtaga actactattc aaaaaatctt attaatgaga taatgagatt
      301 acatcgataa cgttctgatt gcaggcatac ttatctgggt gtgcctctct tttttttaca
      361 actgtacacg gtggcgtatg acgagaaaat atctcacaca ggatgaagtc tacaggctga
      421 tggatgcagc ccagagcatg tcctttcctg aaagaaatcg ctgtctaatt atgatggctt
      481 tcattcacgg ttttagggcc agtgaacttc ttgatttacg tttatccgat attgatgcat
      541 caggaaaaca acttaatatt cggcgtatta aaaatggttt ctcgacgaca catcctcttc
      601 ttcctgatga atataatttg atcaagttgt ggcttaagca gaggaagtta attgaaaacg
      661 gagttgaagg agactggctt tttctctccc ggaaacgccg ccctatcagc agacaacatt
      721 ttttttctat cattcgtgag gctggaaaac gtgcaggatt agctgtaaaa gcacatcctc
      781 atatgttacg tcatgcctgt ggttttgctc tggctgacaa tggtgtcgat acccgactac
      841 ttcaggatta tctgggtcat cgaaatattc agcatactgt cagatacaca gccagtaatg
      901 ctgctcgttt taaaggggtg tggaaaaaaa agcctcgtta aaacagtacc ttctgattca
      961 aaatgtcaga ttcctacgtt ttcctcaatt cttccataaa aaaacagccc attcaattaa
     1021 ataaaacaca taacatgttg atatttctag ctaaagtaaa ataaccccat gatttgaaat
     1081 gattaatcac aaaggtcata ccagtga
//
LOCUS       DAZFRE010000320         1100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFRE010000320 DAZFRE010000000
VERSION     DAZFRE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.326"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            117..>1100
                     /locus_tag="ACHKUI_005315"
     CDS             117..>1100
                     /locus_tag="ACHKUI_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601282.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGTTGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATV
                     DNKGGMTVTDPDSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNN"
BASE COUNT          330 a          260 c          265 g          245 t
ORIGIN      
        1 atatgtttga ttaggtaaca atctactggc aatattggac gtcctctatg ttttaaataa
       61 tcaattggtc gggtcagggt atccgacctt ctttatattc gccagaagga tttattatgc
      121 aaaggaaaac tctattgtcg gcctgtattg cattagctct gagtggtcag ggttgggcgg
      181 cagatattac agaaatagaa accaccacag gtgaaaagaa aaataccaat gtgacttgtc
      241 cagcagatcc tggaaaactc agtccagaag agcttaaacg cttaccctct gaatgctcct
      301 ctgtagtcga acagaacctg atgccatggc ttgtcacagg tgctgctact gcgttaatca
      361 caaccttagc catagtggaa ctaaacgacg atgatgatca tcatcgcaat aattctccac
      421 ttccaccaac tccacctgat gatgattcag acgacactcc agttcctcca actcctggcg
      481 gagatgagat aataccagac gatggtccgg atgatacccc gacgcctccc aaaccgattt
      541 cgtttaataa tgacgttatc ctcgataaaa cagaaaaaac gttaactatt cgcgattcag
      601 tttttactta taccgagaat gctgacggga ctatttctct gcaagatagc aatgggcgta
      661 aggcgactat taatctttgg cagattgatg aaacgaataa cacggttgcc cttgaaggga
      721 tgagcgcaga tggtgcaacg aagtggcaat ataatcacaa cggtgagctc gttattacgg
      781 gcgacaatac cacagtaaac aacactggaa aaaccatcgt cgacggcaaa ggcaccaccg
      841 gaactgaaat cgcgggtaac aacgcggtgg tgaatcagga cggcgagctg gatgtgagcg
      901 gcggcggtca cggtattgat atcaccggcg acagcgcaac ggtggacaac aaaggcggca
      961 tgactgtcac cgatcctgac tccatcggta ttcagataga cggcgacaag gcggtcgtga
     1021 acaacgacgg tgacaatgcc atcagcaacg gcggcaccgg cacgcaggtt aacggcgacg
     1081 aagccaccgt gaacaataac
//
LOCUS       DAZFRE010000321         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFRE010000321 DAZFRE010000000
VERSION     DAZFRE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.327"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>1097
                     /locus_tag="ACHKUI_005316"
     CDS             <1..>1097
                     /locus_tag="ACHKUI_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601283.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAKPATIELTPGYFQITAVPRLAVYDPTIQFEFWFSEKRITNTAQVEKSARYLG
                     SGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEASGQPSNDGEGYLEIFRGLIDET
                     LLGQALKERIDASALRTEVTQLEEDIRQRMDTDIAEVTRKIGEAENSLTQLVAKKNED
                     QTLAIAQMSQKVDRVSSEISQTVSQGQSENAR"
BASE COUNT          258 a          294 c          350 g          195 t
ORIGIN      
        1 accctgccgg atggtgtgga gacatacggt gtgtggggac tctccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggct ccctgaacag cgtcatccca ccggcagtac agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gctaaatggg acacgccgcg ggtggtgaag
      301 ggcgtgcgct tcagtctgcg cctgaccagt ggtaagggaa cggatgccag actggtgacc
      361 accgccatca ccgcagacac ggagcaccgt ttcagtggcc tgccactggg ggaatacacc
      421 ctgacggtgc gggcgataaa cagttatggc cagcagggtg aacctgccac caccaccttc
      481 cggattaacg cacctgcaaa acccgccacc attgagctga cgccggggta ttttcagata
      541 acggcggtcc cgcgtcttgc ggtgtatgat ccgacgatac agtttgaatt ctggttctca
      601 gaaaaacgca tcacgaacac ggcacaggtg gaaaaatctg cccgttatct ggggtccggc
      661 agtcagtgga ctgtccaggg gagccggatt aagccgggga cggatttctg gttttacgtg
      721 cgaagcgtca acctggtggg aaaatctgct tttgtggaag ccagcgggca gcccagcaat
      781 gatggtgaag ggtatctgga aattttccgg gggctgatag atgagacgct tctgggtcag
      841 gcactgaaag agcgcattga tgcttcagcg ctgcgtacgg aggtcacgca actggaagaa
      901 gacatccgtc agcggatgga cacggatatc gcagaagtga cccggaaaat cggggaggcg
      961 gaaaacagcc tcacgcagct ggttgcgaaa aagaatgagg accagacact ggccatcgcg
     1021 cagatgagcc agaaagtgga ccgggtgagc agtgaaatct cacagactgt cagccagggg
     1081 cagtcagaaa acgcccg
//
LOCUS       DAZFRE010000322         1061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFRE010000322 DAZFRE010000000
VERSION     DAZFRE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.328"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(23..406)
                     /gene="traM"
                     /locus_tag="ACHKUI_005317"
     CDS             complement(23..406)
                     /gene="traM"
                     /locus_tag="ACHKUI_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO6601284.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            727..>1061
                     /locus_tag="ACHKUI_005318"
     CDS             727..>1061
                     /locus_tag="ACHKUI_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6601285.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGI"
BASE COUNT          327 a          228 c          195 g          311 t
ORIGIN      
        1 caaagtcaaa atttatttcc ctttattcat catcattttt tggaaagaac ctttccatct
       61 cagccgacac cttctctctg atatcttcaa ccatattggc atattcaaac tttggatttc
      121 ctgagacatg aggactgaga gactcaatac ccaaaatttt cgctactgat gactgtgttt
      181 ttacaacgca ttcaagaagt agtttattaa actcggcctg attaaatgca gactctttgc
      241 gctccatctg agcctcatat acacgaagac ccagttcaag aagcattgaa gccgttgctg
      301 aaaaactgac atctttttct ctcgccccct cctgtcgacg tttttcaata atcgcgttta
      361 ttttttcata agcatcattg ctgatataca gattcacctt agccattaga tattaccttt
      421 tttaaacata aaacaaagtt aatttcgaaa cactagaatt catagtgcaa acagggacgc
      481 accgctagca gcgcccctga cggtatccta taaaaaaaca caccgcgccg ctagcagcac
      541 ccctaatata aaataatgtt ttttataaaa atagtcagta ccacccctac aaagcggtgt
      601 cggcgcgttg ctgtagctgc gttaacgacg ctgctttaaa aaaatcagat ttaaacaata
      661 taaatccaca aatacaactc aatgatatta aagataaatc agcaaaaact tgtttttgcg
      721 tagtgtgtgg tgcttttggt ggtgagaacc accaacctgt tgagcctttt tgtggagtgg
      781 gttaaattat ttacggataa agtcaccaga ggtggaaaaa tgaaaaaatg gatgttagcc
      841 atctgcctga tgtttataaa tgagatctgc catgccactg attgctttga tcttgcaggc
      901 cgggattaca aaatagatcc tgatttactg agagcaatat catggaaaga atcccgttac
      961 cgggttaatg ccatcggtat taatccggta acgggatatg gcagcggact gatgcaggta
     1021 gattcccagc attttaacga actggcccgc tatggaatta a
//
LOCUS       DAZFRE010000323         1061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFRE010000323 DAZFRE010000000
VERSION     DAZFRE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.329"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            195..455
                     /locus_tag="ACHKUI_005319"
     CDS             195..455
                     /locus_tag="ACHKUI_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601286.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            452..961
                     /locus_tag="ACHKUI_005320"
     CDS             452..961
                     /locus_tag="ACHKUI_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601287.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLPAGGTSATDLAV
                     ELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVR
                     VQINESAAP"
BASE COUNT          243 a          286 c          319 g          213 t
ORIGIN      
        1 cgcccactgt gagtcatatt cgccctgact ttccagaacc atacgaatcg ccctctgacg
       61 gacttcgggg gaaaaacgag tatttttagt catcctgttt acctctttct cagggagttt
      121 agtctccagg attcccgggg cggttcagga tatgattttt acctgtcaga acattatcag
      181 atggaacgta caggatggat caggaaatga cgttttcact cagctatgaa caactgaccc
      241 ggtttgcgga aaaacgcatc cgggaatgca atctggacag ccacggcgta acttatctct
      301 gcgagtcagc caaagccgga gccgtgctga tcttctggca tgaactggcc ataaatggct
      361 atacctcaat gaatgccata aagagacagg agatcattga cgccgaccat cagcgcctca
      421 gaaagctcat ctggccggag gatgactgga aatgactcag agccgccgac cgtcgccact
      481 gcagcggaga gtgctgattg tgctggctgc ccttgatgaa aaacggccag gacccgtgct
      541 gacgcgggac atagaacggg tgctggagca aagcggggaa gccccggtct acgggccaaa
      601 cctgcgcgcc tcctgccgtc gtctggagga tgccggctgg ctgcgtaccc tgcgcgctcc
      661 gaacctgcag ctggcagtgg agctgacgga cgccggccgg gccgtggcac agcctctgct
      721 gcccgcaggc ggcacttctg ccactgatct ggcggtggag ctgaacggca taacgtatca
      781 ggcctgccgt ggggattttg tggtgcgtct cgacggcagc acctgtctgc agttatggaa
      841 taaagaaggc agggtggtac gccgggaggg cgatccgctg gaagtggcgc agtggctgca
      901 ggcctgtcac gatgcaggaa tggaggttcg cgtacagata aatgaaagtg ccgctccgta
      961 agaacacgcc tgtgcacggt ttacacatca tcccatggtg gcagccggtt aactttcctg
     1021 cgccaccagt ggaaagaggc catgagcagt ccggacaaaa a
//
LOCUS       DAZFRE010000324         1042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFRE010000324 DAZFRE010000000
VERSION     DAZFRE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.330"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..985
                     /locus_tag="ACHKUI_005321"
     CDS             <1..985
                     /locus_tag="ACHKUI_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6601288.1"
                     /translation="YHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVK
                     QLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIA
                     KKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVA
                     RVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVG
                     NSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPE
                     EDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
BASE COUNT          317 a          183 c          259 g          283 t
ORIGIN      
        1 gtatcatcat gattttaatg aaaaaatagg tttctggtat gtgatagccc tcgcaggaca
       61 gtctaatggt atggcctacg gtgaaggtat tcccctgcca gatacactcg acaaacccga
      121 gtcccgtgta aaacaactgg ctcgtcgaaa aacaatcaca ccaggaggaa aagagtgtaa
      181 attcaatgag attattccgg cagatcactg cctgcatgat gttcaggata tgagtggata
      241 tcaccatccg gctgctgatt tgcataaagg ggaatatggt tgtgtcggac agggattgca
      301 tatagctaaa aagttactgc catacatacc tgagcaagca gggatattac tggttccttg
      361 ttgcagagga ggtgccgcct ttactgttgg agctgaaggt atgtatgtac ctgatacagg
      421 ggcaacagct gatgcaatgc ggtggggcac cggaacagca ctctatgagg acttagtagc
      481 ccgggttaag gtagctctgg aatataacag aaagaataaa ctgctatctg tctgctggat
      541 gcagggggag tttgatctta tgtcaccaga ttatgaaaag catcctgact tgttttatca
      601 gatggtaact tctttcaggt ctgagttatc tgaatactca tctcagtgtg ttggtaattc
      661 gtcagaaaga gttccatggt tgtgtggtga tacaacctgg tactggaagg aatcttatca
      721 aaaagaatat gattttatat atggacatta cagacaacgg actgatgatg aaatacattt
      781 tctcagcttt caggatagta accgacatga attaactaat gagccagaag aagatgccga
      841 tgatttatct gtcgggtatc tggggtcatc atggaggaca gaattaagct ggacaacgtc
      901 acagagaagt acacatttta attcaatggc cagaagggga gttattgctg aatgttatgc
      961 gcaaaaaata agaaattatt tgtagacaag ttcaggatat atctacagct ctgacatgat
     1021 tctctgagag acatgacgga gt
//
LOCUS       DAZFRE010000325         1011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFRE010000325 DAZFRE010000000
VERSION     DAZFRE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.331"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(662..>1011)
                     /locus_tag="ACHKUI_005322"
     CDS             complement(662..>1011)
                     /locus_tag="ACHKUI_005322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601289.1"
                     /translation="KINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNFLVAI
                     IMLILRSLAIIPADSSAYAMRLLHSFLSWRYGGTQEMIFSSTFVGKYSFGHYDLNLPQ
                     SQPFAGRYGKQTK"
BASE COUNT          307 a          181 c          199 g          324 t
ORIGIN      
        1 aggtctcctg tgaaaaatct cttttcacat tatttaaata aacagagaac cagattaaat
       61 acctgagtat aaaatctctt ctgatgttta attgatttga atgttcgtaa gctatatcac
      121 ttactcaatc cattttaccc agagtcataa aatagaatgt ctgttgatct cattttcata
      181 ttcctttcag actgaagtgc gttttcatca cccgatgaga aagaactgaa gtgatgtttg
      241 ccatagattg cgagatgccc tgtctcattg actatcttgg tgcaccttta tacctgttat
      301 accagatcaa aaatcacgca atccatacaa caaaaccaga tttgcaattc gtgtcacaaa
      361 atatgtcgat ctttttctaa gaggaagatg ccatgtgaag ccagacgaac acttgcggtg
      421 gtcttcaaaa actaaagatc ttagtttaac tatttgtttt ataaataatt tgtttagagt
      481 ctaaacaagg ggagctttgc aagctaactc agtgagcttg gtgaaaatca gtgtttaccc
      541 gccatcaggc tgagcataat tctcatcatg aaatatgttt cctggtttgt ggcttgtaac
      601 tggtcacttc tgaagtcgat ctggagaggc ttgttgatgt tggtgttttc aggatgatgt
      661 ttcacttagt ttgtttgccg tatcgcccgg cgaatggctg tgattgagga aggtttaagt
      721 cgtagtgacc aaagctatat ttaccaacga atgtagatga aaaaatcatc tcctgcgttc
      781 ccccatatct ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaatcag
      841 caggaataat cgctagggac ctaagaatta gcatgataat agccactaag aaattactgc
      901 gctccatgaa atagccattt tgtggcaaat ggagttgact aataatgtca tatgtgagac
      961 ggctagttga acgaatatta aattttgctg aattttttat gttgatttta c
//
LOCUS       DAZFRE010000326         1001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFRE010000326 DAZFRE010000000
VERSION     DAZFRE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.332"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..606
                     /locus_tag="ACHKUI_005323"
     CDS             <1..606
                     /locus_tag="ACHKUI_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO6601290.1"
                     /translation="SSERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMR
                     NGKILGWAWGAGFVDGIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTL
                     SDYYIKRAEQYHPVIPIGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVP
                     VPPSAESHLNGFNTDQMLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            596..>1001
                     /gene="traP"
                     /locus_tag="ACHKUI_005324"
     CDS             596..>1001
                     /gene="traP"
                     /locus_tag="ACHKUI_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO6601291.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAA"
BASE COUNT          235 a          249 c          302 g          215 t
ORIGIN      
        1 tccagtgagc gtgccattgt acgcacccgc aatatcagtt gcctgaagga cggcaaaact
       61 attgatatgc cgattaaggg gcatgtcagc ttccggggta aaaacggtat caagggcgaa
      121 gtggtgatgc gtaacggcaa aatcctcggc tgggcatggg gcgcgggatt tgttgacggt
      181 atcggtcagg gaatggagcg cgcctcccag ccggctgtcg ggctgggtgc cacagccgct
      241 tacggggctg gtgatgtcct gaaaatgggt atcggtggcg gcgcatcgaa agccgcacag
      301 acgctcagtg actactacat caaacgtgcc gaacagtatc acccggtgat accgattggt
      361 gcgggcaacg aagtgaccgt ggtgttccag gacggcttcc agctgaaaac cgtggaagag
      421 atggcgctgg agcgcacgca gaacagagcg gaagaagata atccggaaag tccggttcct
      481 gttccgccat cagctgaaag tcatcttaac ggctttaata ctgaccagat gctgaagcag
      541 ctgggcaacc tgaatccgca gcagtttatg tccggcagcc agggaggagg caacgatggc
      601 aaataatatg tcgtcccgtc aggcgtgcca tgccgcccgg tatgttgtcg cccgggttct
      661 gcgaggactg ttctggtgtc tgaagtacac cgtcattctg ccactggcca caatggccct
      721 gatggctctg tttgttttat ggaaggacaa caccacgccg gggaaattac tggttaagga
      781 gattaacttc gtaagacaaa cagcacctgc cggacagttc ccggtcagtg aatgctggtt
      841 ttcttcctct gattcttcag gcagatcaga aatacaggat atctgccatt accgcgcggc
      901 tgatgctgca gattatgtca gggagactga ccggtcactg atgcaactgg tcaccgcatt
      961 atgggcaaca ctggcactga tgtatgtctc gctggcagcg a
//
LOCUS       DAZFRE010000327         1000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFRE010000327 DAZFRE010000000
VERSION     DAZFRE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.333"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(369..>1000)
                     /locus_tag="ACHKUI_005325"
     CDS             complement(369..>1000)
                     /locus_tag="ACHKUI_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6601292.1"
                     /translation="YRTMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEF
                     RCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAVERRFGSELPASPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPD
                     GLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQWASDGLSDNRCLEI"
BASE COUNT          230 a          278 c          229 g          263 t
ORIGIN      
        1 ggcatgccag ttaacagcta tccgtatggc gtgttcggtt ttgcgaataa aaaatggtta
       61 ctgtagaagc ggatttccac cacatgtcaa ccatgaatgg tcagtggtgc gagacgactg
      121 ggatgatttg gtcgatgact tcttccggcg aatactgata gaaacattga aatattgtaa
      181 gagaaagcgt tcaaaattct gtggcacgtg aaaaattcta cagcgtcatc accttattca
      241 gtcgaccttc aaaataaacg tttaacagag acaatcacca tttggattgt ccgctgagga
      301 ggcgaacgat ttcctagaca gctttatcat gtcagcccac attgttggcg ctgacaatgg
      361 atttgcccct atatttccag acacctgtta tcacttaacc catcactggc ccactgccgc
      421 agatattccc gtggcgagcg ataacccagc gcactatgcg gatgccattc gttataatgc
      481 tcgaacgcct ctgcaaggtt ctttgctgcc gttaacccgt ctggtttggg catgatactt
      541 atgtagtcac gctttatcgt tttcacgaag ctctctgcta ttccgttact ctccggactc
      601 cgcactgccg tgttcttcgg ttcaagtccc aacatcctgg cgaactgacg tgtttcattc
      661 gcccggtagc atgaaccatt atccgtcagc cactccactg gagacgccgg aagctcgctg
      721 ccaaagcggc gttccactgc tcccagcatg acgtcctgta ctgtttcact gtcgaagcca
      781 cccgttgtga ccgcccagtg cagtgcctca cggtcacagc agtccagcgc gaacgtgacc
      841 cgcagttttt ctccgttatc acagcggaac tcaaacccgt cagagcacca tcgctgatta
      901 ctttctttca cagccactct gccggtatgt gcccgtttcg atggcggtac agcgggtttt
      961 cgctcaagca acagcgcatt ctggcgcatg gtccggtaaa
//
LOCUS       DAZFRE010000328          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFRE010000328 DAZFRE010000000
VERSION     DAZFRE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.334"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..377)
                     /gene="trbB"
                     /locus_tag="ACHKUI_005326"
     CDS             complement(<1..377)
                     /gene="trbB"
                     /locus_tag="ACHKUI_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO6601293.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDV"
     gene            complement(364..648)
                     /gene="traQ"
                     /locus_tag="ACHKUI_005327"
     CDS             complement(364..648)
                     /gene="traQ"
                     /locus_tag="ACHKUI_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO6601294.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
BASE COUNT          272 a          265 c          220 g          239 t
ORIGIN      
        1 atcacgtccg gtggcaccgg taatgcttcc ggaaaggcgg tatccccctg accatccagg
       61 gtgtagggaa aaacggaaaa gccgtactgc tgtgccagct gtttcagtac cgggtcaaac
      121 tggtgacagt aagggcaatg cccctgcata aacagcacca ccttccagtc ggccaggtta
      181 acctgcctgc cgttgctgag gcggaaccag cggggagccg gcttttctgt tctggctgat
      241 gtgtctgctg ccggttgtgc aggctgcacg gccataccct gcggattcca gagtcgttca
      301 atttcatccc gggtggaggc ctgcacacct gtggccatca tcaacaaaat gaccggcagt
      361 aatttagtga gagacatgtc cgccctcctg ctgcccttcc atcatggcct gttgcctggc
      421 ttccagtgcc tcacgctctt ccaggctgac acgtgcaatc catgcatcca gcacctggta
      481 tgcgccgaac agcacaagga tggccgcaat caactccgcc agaaaaaaag ccatccaggg
      541 tttgctgtaa acaagacggg cgacgatatg aaaccagaca cccagggaaa atacccacat
      601 tcccgtaata tcaagccggg gtaaagagaa tctgtattta cttatcatgt taaactccgg
      661 ttattattta catgagagta ttcatgtctg taatattcgc gtttgtgtga tattaatcag
      721 atcaacctca ttattttgta tggtttttat taccgtttta tatgacttag cctgtcataa
      781 gcccccttat aaaaaaccac atcactccgg accatgctga tgacaacata atacaaacaa
      841 acatccttct gaaactgtca aacttcgtga taaccggtat ggtactgact gccattgaaa
      901 caaccggtac ggcacactga ttcataaccg caccacaaag aaacaagaaa ttatctggca
      961 gcatacatac acttatgaca tttactacaa agaaga
//
LOCUS       DAZFRE010000329          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFRE010000329 DAZFRE010000000
VERSION     DAZFRE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.335"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            101..337
                     /gene="ycgZ"
                     /locus_tag="ACHKUI_005328"
     CDS             101..337
                     /gene="ycgZ"
                     /locus_tag="ACHKUI_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO6601295.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            380..652
                     /locus_tag="ACHKUI_005329"
     CDS             380..652
                     /locus_tag="ACHKUI_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO6601296.1"
                     /translation="MKTSDNESIKYEITDQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            681..947
                     /gene="ariR"
                     /locus_tag="ACHKUI_005330"
                     /gene_synonym="ymgB"
     CDS             681..947
                     /gene="ariR"
                     /locus_tag="ACHKUI_005330"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO6601297.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          327 a          154 c          191 g          278 t
ORIGIN      
        1 attgcgaaaa attaatttat cattctgtac acatatttcg tacaagtttg ctattgttac
       61 ctcacttaac attgattaac atttttaaca gaggcgtagc atgcatcaaa attcagtgac
      121 ttcagattct gcaggagcaa tcactcgtta ctttgcaaaa gctaacttgc ctactcagca
      181 ggaaactctc ggggaaattg tgactgagat tttgaaagat ggacgtaatc tgagtcgaaa
      241 gtcgctttgt gccaaacttc tttgccgact ggaacaagcg acaggggaag aggaacagaa
      301 acattataat gcactaattg ggttgctttt tgaataatgc aacagcctga ttatttaaca
      361 ggttagctat gaagtcgtta tgaagacatc tgataatgaa agtataaaat atgaaattac
      421 tgaccaggcg gtgctccaga tactgcgcat gaagataaat ttttcgttgc agactcttat
      481 taagcaatta ctcgtaatga aatcggctga ggaaaatact ttccgacgag atttaattga
      541 cagcataatt cgtgatttta gcaacagtga tacaggaggg ccaaaccgaa gaacagcgat
      601 agctgacaat aaaagtatgt tcactggtaa gaaaataaac agaatacatt aaaatttcat
      661 aagtaagatg agaggttacc atgcttgaag atactactat tcataatgca atatctgata
      721 aagcgttatc aagttacttt cgtagttcag gtaatttgtt agaagaagag tctgctgtgt
      781 tagggcaggc tgtgaccaat ttaatgcttt caggcgataa tgttaataat aaaaatatta
      841 tcttaagtct gatacactcc ctggaaacaa caagtgatat tctcaaagct gatgtgatta
      901 gaaaaacact ggaaatcgtg ttgcgataca cagctgatga tatgtaactt
//
LOCUS       DAZFRE010000330          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFRE010000330 DAZFRE010000000
VERSION     DAZFRE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.336"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            257..859
                     /gene="fimB"
                     /locus_tag="ACHKUI_005331"
     CDS             257..859
                     /gene="fimB"
                     /locus_tag="ACHKUI_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6601298.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          278 a          168 c          203 g          278 t
ORIGIN      
        1 gatgagggtt attattagat tcgtatccga ttgataaata tataaaggta catagcatgc
       61 aagagcatgg cgtttgtatg gcaacgttat tataattaac agttgctact ccatttaagt
      121 tcactcagaa gaactggtcc acttacgtta gttattaagc aaacgttcgc ttttataaac
      181 ataatcagga taaaaatgtt ggattatttc taacccagca cagctagtgc gcgtctgtaa
      241 ttataaggga aaaacgatga agaataaggc tgataacaaa aaaaggaact tcctgaccca
      301 tagtgaaatc gaatcactcc ttaaagcagc aaataccggg cctcatgcag cacgtaatta
      361 ttgtctgact ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag
      421 gatttcggat attgatctta aggcaaagtg tatatatatc catcgattaa aaaaaggctt
      481 ttcaacaacg cacccgctat tgaataaaga agttcaggct ttaaaaaact ggttgagtat
      541 ccgtacttcg tacccgcatg ctgagagcga gtgggtattt ttatcacgta aggggaatcc
      601 gctttctcgg caacagtttt accatattat ctcgacttcc ggtggtaatg ccgggttgtc
      661 actggagatt catccgcaca tgttacgcca ttcgtgtggt tttgctttgg cgaatatggg
      721 aatagatacg cgacttatcc aggattatct tgggcatcgc aatattcgtc atactgtctg
      781 gtataccgcc agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca
      841 gcgtcacgct gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta
      901 ttcttacgat atatcaaaaa tgattaa
//
LOCUS       DAZFRE010000331          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFRE010000331 DAZFRE010000000
VERSION     DAZFRE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.337"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            495..725
                     /locus_tag="ACHKUI_005332"
     CDS             495..725
                     /locus_tag="ACHKUI_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601299.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            777..>924
                     /locus_tag="ACHKUI_005333"
     CDS             777..>924
                     /locus_tag="ACHKUI_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601300.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHD"
BASE COUNT          244 a          259 c          241 g          180 t
ORIGIN      
        1 ccgtatgagt ccctgtcgcc gggggctgat acagccacaa cctgccgcac tgactgtcca
       61 gggtaaatga acagcccaca aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct
      121 gcccaggaca ggtgcgtcag gccgtggcag tgatgcccct tgcggcgaag gttctcaaac
      181 gggtgaagtt cataccacag ggaatacagc agcaggttcc cgcgaaacgt caaaaccgcg
      241 gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg atttatttca gactccggca
      301 atacgcattt atgtgttctg tcagcatcct gctggcaggt aatgagaacg caggaacaga
      361 aggaggaata caccggagaa cgtcagacgc agatatgaaa aaagcaggcc ggggaggacc
      421 tgcttaatcc acaccgcaaa aactgttcga gcagtttctg cggttccatt caacagtcat
      481 aaggagtttc atttatgacg gtaagtattg tttcaccttc cgcagcggct gtaaagcccc
      541 gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac cccgtgctga
      601 aagccttcgg gcgacacata gcccggagtt ttcaccgggg gcgtggtgta cacatccccg
      661 ccatgaaaaa cacggccttc gggcaggtgc tccgcacact cgaactgaaa cgcgctttta
      721 actgatgagg tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa cattttatga
      781 cattagcagc aatcaccatg accgccccgg aagccgccag ccctgtgcag atgtaccgcg
      841 cgacctactc accggatgac aacaaactgc gcctgtatgc cgcgtcacgt cttgacccgg
      901 agacgtataa aaaagtgcat gatg
//
LOCUS       DAZFRE010000332          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFRE010000332 DAZFRE010000000
VERSION     DAZFRE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.338"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..610
                     /locus_tag="ACHKUI_005334"
     CDS             <1..610
                     /locus_tag="ACHKUI_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6601301.1"
                     /translation="RVIYKSTDEMSVVKATNFSEKNYWLLRSWVSNYFDDQKNECFIV
                     TPPLHRIEPNDEIQLQIKAIREEKIPDDRESLFRLNVLAIPPKTFLSTDGKEIKGHLQ
                     FAINSRIKLIYRPARIGEPYDITKKLYVHKFNDLVAIKNPTPFFVTLVNISIDGKTIH
                     RDIDEVIKPYSEINIDARNPKWIEFSTVNDKGGTTPPIKINL"
     gene            607..>923
                     /locus_tag="ACHKUI_005335"
     CDS             607..>923
                     /locus_tag="ACHKUI_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFO6601302.1"
                     /translation="MTKMKKQNLLGCAFIVVTYSSYAASSDNATFDPNFLKMSDGEEA
                     RHLDLKYFMRPGGMSPGTYTVAIFLNGVKVSTEESVEFISDKKQEGVTFPFFNQFILA
                     SWGV"
BASE COUNT          331 a          129 c          173 g          290 t
ORIGIN      
        1 tcgagttata tataaatcaa ctgatgaaat gtcagttgtt aaggcaacca atttttcaga
       61 aaaaaattac tggctgttgc ggtcatgggt gtcaaattat tttgatgatc aaaaaaatga
      121 atgttttatt gtgacaccac cacttcatag aattgagcct aatgatgaga ttcaattaca
      181 gataaaagca attagagagg aaaaaatacc ggatgacaga gagtcattat ttagacttaa
      241 tgttttggct attccaccaa aaacattttt gtcaacagat ggaaaagaga taaaaggtca
      301 tctgcaattt gcaataaata gtaggataaa attgatatac cggccagcaa gaataggaga
      361 accttatgat attaccaaaa aattatatgt ccataaattt aatgatttgg ttgcaatcaa
      421 gaatccaacg cctttttttg tgacattggt caatatttct attgatggta agaccataca
      481 tagagatatt gatgaggtta taaaaccata ttctgaaatt aatattgatg cacgaaatcc
      541 aaaatggatc gagttttcaa ctgttaatga taaaggtgga acaacaccac ccataaaaat
      601 aaatttatga caaaaatgaa aaaacaaaac ttgttaggtt gtgcttttat tgttgttaca
      661 tattcctctt atgccgcatc aagtgataac gcaacctttg atcctaattt tttaaagatg
      721 tctgacggtg aagaagcaag acatttagat ttaaagtatt ttatgcgtcc gggcggaatg
      781 agccctggta catacactgt ggcaatattt cttaatgggg taaaagttag tactgaggaa
      841 tctgttgagt ttatttcaga taaaaaacag gagggggtta catttccatt ctttaatcag
      901 ttcattttag ctagttgggg agt
//
LOCUS       DAZFRE010000333          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFRE010000333 DAZFRE010000000
VERSION     DAZFRE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.339"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..423)
                     /locus_tag="ACHKUI_005336"
     CDS             complement(<1..423)
                     /locus_tag="ACHKUI_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6601303.1"
                     /translation="MKTISHNSTTSSVSVTTASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYY"
     gene            complement(478..>918)
                     /locus_tag="ACHKUI_005337"
     CDS             complement(478..>918)
                     /locus_tag="ACHKUI_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601304.1"
                     /translation="CVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFDRIEEKRDAMQ
                     SLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENML
                     KGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          212 a          257 c          237 g          212 t
ORIGIN      
        1 gtaataatgg accgtcatgg cataacactc cgtacgacag gcatgatgac tgtacgtcat
       61 cagacaggca gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc
      121 attgaacagt acccatgttt cgtcatcatc gtcatccggt tcgggtgcca taaatgcccc
      181 gccgttgttc agggtgtaca gattccagat accaccgcag tagtcttcac acagacggtc
      241 catccagccg aagacacggg gctccagggc cacccactgt ggaatgaggc caaaatgctg
      301 cggccagaag ctgatgcgct gttcatcagg gactatggtg gcaaccaact gaggctggtc
      361 attccctgat gcagtggtta cggaaacaga agatgtggtg gaattatgtg aaatggtttt
      421 catgaaatga ctccttaaag gacaaataaa tgcgtggttt tcagggtaat ggctgaatca
      481 gcgcaggctc tccagcagcg tttccgccat cacccacaat gcgcggttga gcttaatgtc
      541 agtgtcaatg ctgtgaatgg cacgggtatg gatacgtttt cctttagcgc tgcgacctga
      601 aattccgcct ttcagcatat tctcctgaat ggtctgataa gcactccaca ggtccttacc
      661 gtaatcctcc cggcgtcgtg gtgtcagaat gtcggcggtg gtgacgggct gatgttcgtc
      721 accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg gcggcagaat
      781 cagcgactgc atggcatcac gcttttcctc aatccggtca aaaaccccca ccacctcgta
      841 agccccttca ataactttct ccaccacatt tccccggtgc ggaacacgca cttcccccag
      901 agactgacca cagacgca
//
LOCUS       DAZFRE010000334          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFRE010000334 DAZFRE010000000
VERSION     DAZFRE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.340"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..482)
                     /gene="traK"
                     /locus_tag="ACHKUI_005338"
     CDS             complement(<1..482)
                     /gene="traK"
                     /locus_tag="ACHKUI_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6601305.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     P"
     gene            complement(469..>885)
                     /gene="traE"
                     /locus_tag="ACHKUI_005339"
     CDS             complement(469..>885)
                     /gene="traE"
                     /locus_tag="ACHKUI_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO6601306.1"
                     /translation="PMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQ
                     ALLQYIRPGAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWI
                     GNSEPFTDIKHYILILKRENGVTWLDNFGETDDEKK"
BASE COUNT          214 a          235 c          224 g          212 t
ORIGIN      
        1 ggaacctgat accagcctgc aggtaatttt ccgccacgga cagcctggct gatggtcaca
       61 agcagggatt cgtaaggcgt ggacgtttcc cacgctccgg cttcttctcc ggttccgcgc
      121 aggtcactga ccagctgaat ggtacgcccc gcgccttcac ggggaacagc ctgaatggaa
      181 aggttcagac cacgctctgt ttccagaatg aacgtaaagg gctttttgtt gacggtggcc
      241 accaccacac cgccggaggc ggtctgctca ttattggtca gggcaccatc cagactgttc
      301 acggcgataa tccggtcacc gggaacggta aacagattcg ggctggtatt gctgatggca
      361 atactggcct gaccaccgtt caccattggc accacagtgg gggccagcgt accgtttgcg
      421 gccatcacgc tgcaggaaaa aaacaggccg ccgaatatta ttgccgtatt attttttctc
      481 atcgtctgtt tccccgaaat tatccagcca ggtcaccccg ttttcccgct tcagaataag
      541 gatgtaatgt ttgatatcag taaaaggttc ggagttacca atccatgttt tccgaattcc
      601 acggatttcc acacgaccat actgaggcca gacacggaca ctggtctgga aaaaagcaga
      661 attcacgtta tcttttttaa tgagtttcgc ttcttcagcc agaataactt tcatctggtt
      721 ctgtgcaccc gggcggatat attgcagaag cgcctgatgt gaagcatcga cgatttccgg
      781 tgagacattc agacggaggg caataaatga cagcgccatc tgctgcaaat aagaggcatc
      841 ggcactgttc tgtgacacgg caaagggcac attaaatccc attgg
//
LOCUS       DAZFRE010000335          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFRE010000335 DAZFRE010000000
VERSION     DAZFRE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.341"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..482)
                     /gene="traK"
                     /locus_tag="ACHKUI_005340"
     CDS             complement(<1..482)
                     /gene="traK"
                     /locus_tag="ACHKUI_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6601307.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     P"
     gene            complement(469..>885)
                     /gene="traE"
                     /locus_tag="ACHKUI_005341"
     CDS             complement(469..>885)
                     /gene="traE"
                     /locus_tag="ACHKUI_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO6601308.1"
                     /translation="PMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQ
                     ALLQYIRPGAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWI
                     GDSKPFTDIKHYILILKRENGVTWLDNFGETDDEKK"
BASE COUNT          206 a          232 c          234 g          213 t
ORIGIN      
        1 gggacctgat accagcctgc gggtaatttt ccgccacgga cggcctggct gatggtcaca
       61 agcagggatt cgtaaggcgt ggacgtttcc cacgctccgg cttcttctcc ggttccgcgc
      121 aggtcactga ccagctgaat ggtacgcccc gcgccttcac ggggaacggc ctgaatggaa
      181 aggttcagac cacgctctgt ttccagaatg aacgtaaagg gctttttgtt gacggtggcc
      241 accaccacac cgccggaggc ggtctgctca ttattggtca gggcaccatc cagactgttc
      301 acggcgataa tccggtcacc gggaacggta aacagattcg ggctggtatt gctgatggca
      361 atactggcct gaccaccgtt caccattggc accacggtgg gggccagcgt accgtttgcg
      421 gccatcacgc tgcaggaaaa aaacaggctg ccaaatatta ttgccgtatt attttttctc
      481 atcgtctgtt tccccgaaat tatccagcca ggtcaccccg ttttcccgct tcagaataag
      541 gatgtaatgt ttgatatcag tgaaaggttt tgaatcacct atccaggttt taagcacacc
      601 acgaatttcc acacggccat actgaggcca gacacgaaca ctggtctgga aaaaggctga
      661 gttcacgtta tcgtttttaa tacgcttcgc ttcttcagcc agaataactt tcatctggtt
      721 ctgtgcgccc gggcggatat attgcagaag cgcctgatgt gaggcatcga cggtttctga
      781 tgaaacattc agacggaggg caataaatga cagcgccatc tgctgtaaat aagaggcgtc
      841 ggcactgttc tgtgacacgg caaagggggc attaaatgcc attgg
//
LOCUS       DAZFRE010000336          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFRE010000336 DAZFRE010000000
VERSION     DAZFRE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.342"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..659)
                     /locus_tag="ACHKUI_005342"
     CDS             complement(<1..659)
                     /locus_tag="ACHKUI_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6601309.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FP"
BASE COUNT          235 a          196 c          199 g          254 t
ORIGIN      
        1 gggaactcat agttaccggt gtaggtcacg ccgaaagtcg gcaccacgcc gatggtgttt
       61 tcgtcgcagg cttcaatcat gcgtttcggg tccataaaca actgaccggg gcgcataggg
      121 atctcacgca actccacatc ccagtagcgg gcgaatttat gccagcagat ttgtaccgga
      181 ccgcacacca ggttcggttt gttagtcggc ttacctgcag cttccatacg cttgcgccaa
      241 cgccatttca tcgccatccc gccgagcata caggcctcgg aagaaccaat ggtgttggtg
      301 ccaacggcct gaccattttt cggtgcaggc gcatgccaca gatcggcaac catgtttacg
      361 caacgcagat cgattgctgc agattgcgga tattcttctt tgtcgatcca gtttttgtta
      421 atggataaat ccatcaactt atggacatta tcgtcgtccc aggtctggca gaaagtggcc
      481 aggttctgac gagcgttgcc atcaagatat aattcatcat tgataatctg gaatgcgaca
      541 tcatcacgca tttcgtgcag cggaaaacgt tttgattctg cgatagtgga aatagacttc
      601 gcaccaaaac gtgaatcgag tagttccgac cttaaatccg ttacttgctt cttatccatt
      661 ttaaactcct taaaatgatt ggatcgcatt aaaaaagtag gatttatcga taaagtaagc
      721 aagtgcaaag gactcggttt taaataacaa aatcctaatg ttatttatcg cgagatattg
      781 cgtgaataat gtatttttat taaaaaacaa cataatagag aattctgtgt ggaggtaagg
      841 tgttttatgt tgttatttta tgtgttttct atcgtctcct gaat
//
LOCUS       DAZFRE010000337          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFRE010000337 DAZFRE010000000
VERSION     DAZFRE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.343"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(33..629)
                     /gene="fimE"
                     /locus_tag="ACHKUI_005343"
     CDS             complement(33..629)
                     /gene="fimE"
                     /locus_tag="ACHKUI_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6601310.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          281 a          200 c          161 g          242 t
ORIGIN      
        1 gtttttttta actttattat caataagtta aatcaaacct cttctctttt taatttttcg
       61 tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact ggcggtataa
      121 cgcacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg agtatctgca
      181 ccacgctccg ccaattcata accgcaagca tgccttaaca tatgaggatg agtctgcgtt
      241 acggttccag cttcaatacc ggcatcgcga ataatgcgat aggcctgctg gcgagaaagc
      301 cgactcccgc ggcgagaaat aaatatagcg tcagtccggt cagcgccttt ccagttagca
      361 cgttcctggg tccagcgttc cacggcttca cgctcatcaa aacgtaacgg gtgaacggta
      421 gaaaatccgt tcttcagtcg gcgaatattt attctacctt cattaaggtc aaggtcctga
      481 taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa cagaataaga
      541 caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc ctgaacttct
      601 ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga gatatatttg
      661 aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata aaaagataac
      721 cacacacgga gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa aaataaaata
      781 tcactgatgt gacaacatct attttcattg atttgattca atataacaat agattgcagt
      841 ggattaactt ccagttgctt aaaaaataaa gaaaaaagtg cgga
//
LOCUS       DAZFRE010000338          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFRE010000338 DAZFRE010000000
VERSION     DAZFRE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.344"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>872
                     /locus_tag="ACHKUI_005344"
     CDS             <1..>872
                     /locus_tag="ACHKUI_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO6601311.1"
                     /translation="QLDKLRRMNFGSCSEKVSRRIAQMEADLKALQKESDTLTGRVDD
                     PAVQRPLRQTRTRKPFPESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSA
                     FRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSE
                     IYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKK
                     TKTGRLWTYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNE
                     LYRDDRITEAACWA"
BASE COUNT          175 a          259 c          276 g          162 t
ORIGIN      
        1 cagctggata agctgcgccg gatgaacttc ggcagctgct ccgaaaaagt ctcccgtcgt
       61 atcgcacaga tggaagctga cctgaaggca cttcagaaag aaagtgatac ccttaccggt
      121 cgggttgacg acccggccgt gcagcgcccg ctgcgtcaaa cccgcacccg caaaccgttc
      181 cccgaatcac tcccccgcga tgaaaaacgg ctgctgccgg cagcgtcatg ctgcccggaa
      241 tgtggaggct cgctgagcta tctgggtgag gatgccgccg aacagctgga gctgatgcgc
      301 agcgccttcc gggttatccg gactgtacgt gaaaagcatg cctgtactca gtgcgatgcc
      361 atcgtgcagg cccccgcgcc ttcacggccc atcgagcggg gtatcgccgg accggggctg
      421 ctggcccgcg tgctgacctc gaagtatgca gagcacaccc cgctgtatcg ccagtcagaa
      481 atatacggtc ggcaaggtgt ggagctgagc cgttcactgc tgtcgggctg ggtggatgca
      541 tgctgccggc tgctgtctcc gctggaagag gcgcttcagg actatgtgct gactgacggt
      601 aagctccatg ctgatgacac gcctgtcccg gtgctgttgc caggcaataa gaaaacaaag
      661 accgggcggt tatggaccta cgttcgtgat gaccgtaacg ccgggtcaac gctggcgccg
      721 gcggtgtggt tcgcttacag cccggacaga aaaggcatcc atccgcagac ccatcttgcg
      781 gggttcagtg gtgtactgca ggcggatgca tacgccgggt tcaacgagct gtaccgggat
      841 gaccggataa cggaagccgc ctgttgggct ca
//
LOCUS       DAZFRE010000339          868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFRE010000339 DAZFRE010000000
VERSION     DAZFRE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.345"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            70..366
                     /locus_tag="ACHKUI_005345"
     CDS             70..366
                     /locus_tag="ACHKUI_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601312.1"
                     /translation="MDITPILHAICAVAVQGLVGCITGDWAYGAAIACTFFVAREYTQ
                     AEYRWIKRFGDGHRHKMPWWGGFDPHAWDVASLMDFVVPVVACAGLYGCMLIFS"
     gene            complement(484..816)
                     /locus_tag="ACHKUI_005346"
     CDS             complement(484..816)
                     /locus_tag="ACHKUI_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6601313.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWVETDDAGGTIPVTMTEQISPY
                     HQCMQGTNQKNPRCVALIGQPGVNAYCSIYEHRSSTCREFPMSGEHGIVNEACNRARA
                     KYGLPPLY"
BASE COUNT          199 a          176 c          252 g          241 t
ORIGIN      
        1 gattccgtgt tccgtgagtg ggtggttaca ccagagcgcc cgctcaggga gtggttttaa
       61 ggatagcaga tggatatcac gccgatactt catgcaattt gtgctgtggc ggtacagggg
      121 ctggttgggt gcattaccgg tgactgggct tatggtgcag ccattgcctg tacgtttttt
      181 gttgcccgcg aatataccca ggcagaatat cgctggatta agcgttttgg tgatggtcac
      241 cgacataaga tgccgtggtg gggcggtttt gatccgcacg cgtgggatgt ggcaagcctg
      301 atggattttg tggtgccggt ggtggcctgt gcgggattgt atggctgtat gctgattttt
      361 agctgaaaaa aagggagcgc tcaggctccc caatcggaag aaatcgcttg aaacgacaag
      421 cttgttgtta cgccctctaa gaatgttata taaaacatgc cattgccgtg attatgatcg
      481 cgtttaataa agagggggta gcccatattt tgcacgagcg cggttgcatg cttcattcac
      541 gatgccgtgt tctccggaca tcggaaattc tctgcaggtg gatgagcgat gttcataaat
      601 gctgcagtag gcattgactc ccggttggcc gatcagggcc acacagcgag gatttttctg
      661 gttagtgccc tgcatgcatt gatggtaggg agagatttgt tctgtcatcg ttacggggat
      721 ggtgccgcca gcatcatctg tttcaaccca gtaaaaagag acgcggaaaa atgcacaaca
      781 ggcaccacac gtcatacatg gattaggatt gttcatactt ttattgcttt agcatcaatg
      841 agttattatt acagatgtaa ctgcagaa
//
LOCUS       DAZFRE010000340          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFRE010000340 DAZFRE010000000
VERSION     DAZFRE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.346"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..861)
                     /locus_tag="ACHKUI_005347"
     CDS             complement(<1..861)
                     /locus_tag="ACHKUI_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6601314.1"
                     /translation="MKSRYITPVVTIFDEQGRIDPEQNTQIYDFIKNHVSGFVVMGST
                     GEFFSLNMENARQMIKTCSEFSRGDMKVYAGASRMDVNESISLANYAWEQGLDGVMII
                     SPYYFPLDDEAIFDFYSTIASQTPAHIFIYNFPERTGYSVSPEICLRLADKYPNIIGM
                     KDTVPDAFHTAQVINTVKKNIPHFEVYAGYDNNFAHNILSGGDGCIGGLSNIVPEFFA
                     SWMQAFTNDDLLAVAQHQQIVDQLMAVYSVNSPFIPTFKKALQLKGVIQSERCSSPFN
                     ILDDIQSRRLQ"
BASE COUNT          264 a          182 c          177 g          241 t
ORIGIN      
        1 ctgcaatctc cgggattgaa tatcatcaag tatattaaat ggtgaagaac accgctctga
       61 ttgaataacc ccctttaact gaagggcttt tttgaaagtg ggaataaaag gagaattcac
      121 gctgtaaacg gccattagtt gatcgactat ctgctgatgc tgcgcgactg caagtaaatc
      181 gtcattcgtg aatgcttgca tccaggaggc aaaaaattca gggacaatat tggataaacc
      241 accaatacag ccatctccac cagatagtat gttgtgggca aaattattgt catatcctgc
      301 atacacttca aaatgaggga tatttttctt tacggtattg ataacctgcg cagtatggaa
      361 agcatcaggg acggtatctt tcataccaat aatattggga tatttatctg ccagtcgtaa
      421 acaaatctct ggtgatacgc tgtacccggt tctctccggg aagttataaa taaaaatatg
      481 agccggggtt tggcttgcaa tcgtactgta gaaatcaaaa atagcttcat catccagagg
      541 gaaataataa ggactgatga tcatcacacc atccaatcct tgctcccagg cgtaattggc
      601 cagtgatatt gattcattga cgtccatgcg actggctccg gcataaacct tcatatcgcc
      661 tcgggaaaac tcactacagg ttttaatcat ttggcgggcg ttttccatat taagagaaaa
      721 aaactcacca gtactgccca tcacaacaaa cccggacacg tgatttttaa taaaatcata
      781 aatctgagtg ttttgctcag gatcgattcg cccttgttca tcaaatatgg tgacaacggg
      841 ggttatgtaa cgacttttca tgcc
//
LOCUS       DAZFRE010000341          859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFRE010000341 DAZFRE010000000
VERSION     DAZFRE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.347"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..214)
                     /locus_tag="ACHKUI_005348"
     CDS             complement(<1..214)
                     /locus_tag="ACHKUI_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601315.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRV"
     gene            complement(314..547)
                     /locus_tag="ACHKUI_005349"
     CDS             complement(314..547)
                     /locus_tag="ACHKUI_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6601316.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRIIR
                     NTEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            633..>859
                     /locus_tag="ACHKUI_005350"
     CDS             633..>859
                     /locus_tag="ACHKUI_005350"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6601317.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWHEGKLRPPSTTENNVPELLPIT"
BASE COUNT          226 a          218 c          219 g          196 t
ORIGIN      
        1 gcacacgggt ctggcaggcg aagaacggct gaaagccttc ccgctgcaga ctttccagca
       61 cggtgatggt ggggatatac gtatagcgtt cactgcggga ggtatgccgg tcttccccaa
      121 aaataccggg tacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct
      181 ggttcgcata accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga
      241 tttactggtg taataaatga aaaagccacg tctcccggag aagacgcggc ctgatgggtg
      301 aaatgaatga cgtttattgt ctgagaagac cgtcactggc gagccgcgta ttaagcacct
      361 ttccggcatc gggttcagaa ttccggcagc gccagcgtcg ctgaccttcc gtgttgcgga
      421 taatccgacg gaaatgcatt cccgggtcat cctggataaa cacattgcgg taagctgtta
      481 tgacagcaac cgcctgcccg tatgagaaag atccttcagc caggacatac tctgtgtaac
      541 ccggcatatc tgtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctgagaga
      601 atagtggaca ccaaatatgg tggacgctat ccatgaaatc attaaccgca gtgcgtaaaa
      661 aaagccctaa ttatcccgtt gagttcaaaa tcaaaatggt tgaactctcg catcgaccag
      721 agatctccgt agcgcaactc gctcgtgagc atgggatcaa cgataatttg ctgttcaagt
      781 ggcgccagta ctggcacgaa ggaaaactac gtcctccttc aacaacagaa aacaacgtgc
      841 ctgagctgct cccgataac
//
LOCUS       DAZFRE010000342          850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFRE010000342 DAZFRE010000000
VERSION     DAZFRE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.348"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..658)
                     /locus_tag="ACHKUI_005351"
     CDS             complement(<1..658)
                     /locus_tag="ACHKUI_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601318.1"
                     /translation="MKSDLTIKNRYCTIPQAKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKYAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     LLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFQIAATYL
                     RDLVLFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFICLINNLPARDTDDYKFIS
                     Y"
     gene            complement(661..>850)
                     /gene="tssD"
                     /locus_tag="ACHKUI_005352"
     CDS             complement(661..>850)
                     /gene="tssD"
                     /locus_tag="ACHKUI_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6601319.1"
                     /translation="ERFYTIELQGAHLSNILTCYPHSITHAGNQPEEVIVLNYKNITW
                     KHHIASTESYSCWEERIF"
BASE COUNT          265 a          173 c          128 g          284 t
ORIGIN      
        1 cataagagat gaatttataa tcatctgtat cacgcgcagg caaattatta attaaacaaa
       61 taaaaaaacc caaatctcgt tcaacaccac gattatatct aataccagcc ataatatact
      121 gctcattcgt catatacagt gttgccgaag agggataatc aaaaagaaca agatctctta
      181 aataagtagc agcaatttga aaggtaaaat catcctccat taggcatgtc gctaactcta
      241 actgatttct ggtagttaat tctgaagggt ctagacctag agtaatcgct gcggttcgta
      301 tttgtatacc cattgagcca aatgatgttt tattttcagg catatctaac aatccgatca
      361 ttctttttgt ttcatatatc tgaaacttat aattttcagg catacctcct gactcaatcc
      421 aggccacgcc gcccagtaac tctggacgaa ttccagcggc gtatgcatat ttaattattt
      481 tgtctctatt atattgtaca tatgcatctt tataagcatt taaataatat actcctgaac
      541 ggcgacgcga atcatttttt cctaacttcc agagtaaaac gtccatttca tcccatttac
      601 ggaacttcgc ttgtggaatt gtacaatatc tattttttat agtgagatcg cttttcatat
      661 ttaaaaaatc ctttcttccc aacaactata tgattccgta ctcgcgatat ggtgcttcca
      721 tgttatattt ttataattaa gaacaatgac ctcttccggc tgattacccg catgtgtaat
      781 tgagtgtgga taacatgtca gaatattcga taaatgtgct ccttgcaatt caattgtgta
      841 aaacctttca
//
LOCUS       DAZFRE010000343          840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFRE010000343 DAZFRE010000000
VERSION     DAZFRE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.349"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..832
                     /locus_tag="ACHKUI_005353"
     CDS             <1..832
                     /locus_tag="ACHKUI_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6601320.1"
                     /translation="RRGIISDRLATAILVHAGRNAEFITGKQPDTVKPTGPSGEGTER
                     EPEAPVSNRTLMSLLASGEDLASQGWRYYHKPASGDNVNKNIAEAVVSDAGATGGKAL
                     QLNKPENHIWFLEHDAAGQGVELLKKGGRVSVRFKLPGSLVPNQFALGIYWQLSSLPE
                     GVTLAEEGNDMLMSFFLQTDATNLNAMHHKKPNAKLDTFGVFDNGWHTLAFEFAGNNS
                     IQVTPVLDEKRGTPFTLVKSPASGAADKLQLTGISKAATYTLLIDSVKVEVNNADIAA
                     "
BASE COUNT          217 a          195 c          269 g          159 t
ORIGIN      
        1 acgtcgtggg attatttccg accgtctggc aacggcgatt cttgtgcatg ccgggagaaa
       61 cgctgaattc attaccggaa aacagcctga tacggtgaag cccaccggac cttccggtga
      121 aggtacggag agagagccgg aagccccggt cagtaaccga accctgatga gtctgctggc
      181 gtccggcgaa gacctggcat cacagggctg gcgctattat cacaaaccgg cgagcggaga
      241 caatgttaac aaaaacattg ctgaagcggt ggtcagtgat gcgggggcta cgggaggtaa
      301 ggccctgcaa ctgaataaac cggaaaacca catctggttt ctggagcatg atgcagccgg
      361 acagggagtg gagttactga agaagggggg acgtgtgagc gtacggttta agttgccggg
      421 ttcactggtg ccgaatcagt ttgccctggg catttactgg cagttgtcgt ccctgccgga
      481 gggagtgacg ctggcagagg aaggcaacga catgctgatg tccttctttc tgcagacgga
      541 tgcgacgaac ctgaacgcga tgcaccacaa gaagccgaat gcgaagctgg atacgttcgg
      601 ggtctttgat aacggatggc acacactggc ttttgagttt gccggaaaca acagcattca
      661 ggtgacgccg gtactggatg agaaacgggg gacgccgttc acactggtga aatctccggc
      721 atcaggggcg gcggacaaac tgcaactgac aggcatatca aaggcggcga catatacgct
      781 gctgattgac agtgtgaagg tggaagtgaa caacgcggac atcgcggcat gataaaaaaa
//
LOCUS       DAZFRE010000344          832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFRE010000344 DAZFRE010000000
VERSION     DAZFRE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.350"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>832
                     /locus_tag="ACHKUI_005354"
     CDS             <1..>832
                     /locus_tag="ACHKUI_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6601321.1"
                     /translation="PLDRLSNSLRNNYLNTSVSGSKHSDTQRVGFSGNGLNNQLNYNI
                     YQSFDSRNNSHSGNVDASYVGTYGRVSGGYFYQDNLERFSYGINGGVAIHDEGITLSQ
                     PLSLNGANALLKVERANGIKVQNGIGIYTDFRGYTVVPNLTSYSRNKVSIDTSSLDRN
                     IEVSRSGSTVVPTRGALVKTIFETNIGMKVLAEIKDEKGVHIPFGSVVSIDGTEKKSG
                     FIVDDNGVVYLTGMPNKGTLNVQWGSDNSRKCKSEFNLGEVNSTYLKKIYLTCNYNYS
                     G"
BASE COUNT          264 a          105 c          182 g          281 t
ORIGIN      
        1 tcccactaga taggttatcg aattcattgc gtaataatta tttaaatacc agtgtttcag
       61 gttctaaaca tagtgataca cagagggttg gcttcagtgg taatgggtta aataatcaat
      121 tgaattataa tatctatcag tcctttgatt ccagaaacaa tagtcattct gggaatgttg
      181 atgcatctta tgtaggtact tacggtaggg tgtctggagg atatttttat caagataatt
      241 tagagcgttt ttcttatggt attaacggtg gggttgctat tcatgatgag ggtattacat
      301 tgtcccagcc gttgtcattg aatggtgcca atgctttgct caaagtagaa agagctaatg
      361 gtataaaagt tcaaaacggt attggaatct atactgattt tcgtggttat accgtcgttc
      421 ctaacttaac atcatattcc agaaataaag tgtcaattga tactagttca cttgatagaa
      481 atatagaagt atcaagatct ggcagtacgg ttgttcctac tcgtggggca ttagttaaaa
      541 ctatttttga aacgaatata ggaatgaaag tattggctga gataaaagat gaaaaaggag
      601 ttcatattcc ttttggtagt gttgtttcaa tcgatggaac ggagaaaaaa tcaggattta
      661 tcgttgacga taatggggtt gtttacctca ctggtatgcc taataaaggg actttaaatg
      721 tacagtgggg aagtgataat agtagaaaat gcaaatctga atttaacttg ggtgaggtga
      781 atagtactta tcttaagaaa atatatttga cttgtaatta taattattct gg
//
LOCUS       DAZFRE010000345          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFRE010000345 DAZFRE010000000
VERSION     DAZFRE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.351"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..603)
                     /locus_tag="ACHKUI_005355"
     CDS             complement(<1..603)
                     /locus_tag="ACHKUI_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO6601322.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFT"
     gene            complement(603..>827)
                     /locus_tag="ACHKUI_005356"
     CDS             complement(603..>827)
                     /locus_tag="ACHKUI_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6601323.1"
                     /translation="APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPG
                     TRAVMFSQPASQLLAGARMDVYVIRDGEGN"
BASE COUNT          162 a          267 c          220 g          178 t
ORIGIN      
        1 ggtaaacacc ttacgctgta tccggttagg caccggcaca gactggtacg tcacctgtgg
       61 tggtggcgtg acaccattcc cgggataaaa cgccgtcggc ggtggaacag caacgctgcc
      121 ctgcggcggg aaggatacgg gggtgtttcc tggctgtggt tcgccttccg gcccgggcgg
      181 tgaggcaggc atctgaggta caggctcacc ggtcgccgtg acgggattag cacccagggc
      241 ttttacctgc tctgccaggg cggcattgtc ctgtcccagc ttttcaatac gacgctggtc
      301 atcaccgcgc tgtttgttca ggacatccag ttcacgacgg atttcctcat actgcttctg
      361 catctgcgct gccgtcacct gcatctctgt ggtggcatgc tgacgcacct tgtcatcaaa
      421 ggtcgtatcc accacacctg tcatatccgg cacaggctcc tgttcagcca ccgcctcacc
      481 gttaccggac atgtccacat cagacagata cagtacccca ccaatcgcgg aggctgcacc
      541 gacaaccaca atccccagcc acaggtactg cttgcgtttc acaatggtat tgatactggc
      601 catcagttgc cctccccgtc acggatgaca tacacatcca tgcgcgcacc tgccagtaac
      661 tggctggcag gctggctgaa catcacggca cgggttcccg gctgccagaa gtcactttcc
      721 cggatattca gggcagacag cgttttattt tccacggcaa agcggaccat cttcaggtga
      781 ttccccgtcc atacggcatc tgccactgaa gacagccccg ccggggc
//
LOCUS       DAZFRE010000346          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFRE010000346 DAZFRE010000000
VERSION     DAZFRE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.352"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>827
                     /locus_tag="ACHKUI_005357"
     CDS             <1..>827
                     /locus_tag="ACHKUI_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6601324.1"
                     /translation="DENTGGQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGG
                     QSLNGLAVNTTLDNRGEQWVHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGP
                     ESENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEG
                     GNQYVHNGGTATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGG
                     ALVTSTAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLD"
BASE COUNT          226 a          196 c          269 g          136 t
ORIGIN      
        1 gtgacgaaaa caccggcggg caatggataa aagcgggtgg cacaggcaga aacaccactg
       61 tcaccgcaaa tggtcgtcag attgtgcagg caggaggaac tgccagtgat acggttattc
      121 gtgatggcgg agggcagagc cttaacggac tggcggtgaa caccacgctg gataacagag
      181 gtgagcagtg ggtacacggg ggagggaaag ctgccggtac aattattaac caggatggtt
      241 accagaccat aaaacatggc ggactggcaa ccggaaccat cgtcaacacc ggtgcagaag
      301 gtggtccgga gtctgaaaat gtgtccagcg gtcagatggt cggagggacg gctgaatcca
      361 ccaccatcaa taaaaatggc cggcaggtta tctggtcttc ggggatggca cgggacaccc
      421 tcatttacgc cggtggtgac cagacggtac acggagaggc acataacacc cgactggagg
      481 ggggtaacca gtatgtacac aacggtggca cggcaacaga gacgctgata aaccgtgatg
      541 gctggcaggt gattaaggaa ggaggaactg ccgcgcatac caccatcaac cagaaaggaa
      601 agctgcaggt gaatgccggc ggtaaagcgt ctgatgtcac ccagaacacg ggcggtgcac
      661 tggttaccag tacagctgca accgtcaccg gcacaaaccg cctgggagca ttctccgttg
      721 tggcgggtaa ggctgataat gtcgtactgg aaaatggcgg acgtctggat gtgctgagcg
      781 gacacacagc cactaatacc cgtgtggatg atggcggaac gctggat
//
LOCUS       DAZFRE010000347          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFRE010000347 DAZFRE010000000
VERSION     DAZFRE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.353"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>824
                     /locus_tag="ACHKUI_005358"
     CDS             <1..>824
                     /locus_tag="ACHKUI_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6601325.1"
                     /translation="DDNTGGQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGG
                     QNLRGQAYGTVLNGGEQWIHAGGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPE
                     SENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGG
                     NQYVHKYGLALNTVINEGGWQVIKEGGTTAHTTINQKGKLQVNAGGKASDVTQNTGGA
                     LVTSTAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLD"
BASE COUNT          214 a          208 c          265 g          137 t
ORIGIN      
        1 ctgatgacaa caccggcggt cagcagatag cccggggtgg cactgccaga aacaccacgg
       61 tcaccgcaaa cggtctgcag gatgtcatgg caggtggcag tgccacggat acggttatca
      121 gtgccggtgg aggacagaat ctgcgcgggc aggcttacgg tactgtcctt aacggtggtg
      181 aacaatggat acatgcaggc ggcagtgcat ccggaacggt gattaaccag tctggctacc
      241 agaccattaa acatggcggg caggcaaccg gaaccatcgt caacaccggt gcagaaggtg
      301 gtccggagtc tgaaaatgtg tccagcggtc agatggtcgg agggacggct gaatccacca
      361 ccatcaacaa aaatggccgg caggttatct ggtcttcggg gatggcacgg gacaccctca
      421 tttacgccgg cggtgaccag acggtacacg gcgaggcaca taacacccgg ctggagggag
      481 gaaaccagta tgtgcacaaa tacggacttg cgctgaacac ggtaattaac gaggggggct
      541 ggcaggtgat taaggaagga ggaactaccg cgcataccac catcaaccag aaaggaaagc
      601 tgcaggtgaa tgccggcggt aaagcgtctg atgtcaccca gaacacggga ggtgcactgg
      661 ttaccagcac agctgcaacc gtcaccggca caaaccgcct gggagcattc tccgttgtgg
      721 cgggtaaggc tgataatgtc gtactggaaa atggcggacg tctggatgtg ctgagcggac
      781 acacagccac taatacccgt gtggatgatg gcggaacgct ggat
//
LOCUS       DAZFRE010000348          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFRE010000348 DAZFRE010000000
VERSION     DAZFRE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.354"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>818
                     /gene="traU"
                     /locus_tag="ACHKUI_005359"
     CDS             <1..>818
                     /gene="traU"
                     /locus_tag="ACHKUI_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO6601326.1"
                     /translation="ASADSACEGRFVNPITDICWSCIFPLSLGSIKVSQGKVPDTANP
                     SMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCMVNLGFSLPAFGKTAQGTAK
                     KDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAYLSEIDPTWTDSSLTTI
                     LNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGSQGSMYPFNGWVSNESSPLQ
                     SSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWRYQMVNMYPDSG"
BASE COUNT          175 a          218 c          233 g          192 t
ORIGIN      
        1 gcatctgcgg attctgcctg tgaggggcgt tttgtaaacc cgatcacgga tatctgctgg
       61 agctgtattt tcccgctctc gctgggcagt atcaaagtca gtcagggtaa ggtccccgac
      121 acggcgaacc cgtcgatgcc cattcagatt tgtccggcac cgccgccgct gttcaggcgt
      181 atcgggctgg ccattggtta ctgggagccg atggcgctga cggacgtcac ccggtcaccg
      241 ggatgcatgg tgaacctggg cttcagcctg ccggcttttg gtaaaacggc acagggaacg
      301 gcgaaaaagg atgagaagca ggtgaacggg gcgttctatc acgttcactg gtacaaatac
      361 ccgctgacgt actggcttaa catcatcaca tcgctgggct gtctggaagg tggtgacctg
      421 gatatcgctt atctttctga aattgacccc acctggacgg acagcagcct gaccaccatt
      481 ctcaatccgg aagctgtcat ctttgccaat ccgatagcac agggggcctg cgcagcagat
      541 gccattgcca gtgcctttaa tatgcctctc gatgttctgt tctggtgtgc cggttcgcag
      601 ggcagtatgt acccgttcaa tggctgggtg agtaatgagt ccagtccgtt gcagtcctcc
      661 ctgctggtca gtgaacgcat ggcattcaag ctgcaccgtc agggcatgat tatggaaacc
      721 atcgggaaaa ataacgccgt ctgtaatgaa tatccgtccc caatcctgcc taaagaacgc
      781 tggcgttatc agatggtgaa tatgtatccg gacagcgg
//
LOCUS       DAZFRE010000349          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFRE010000349 DAZFRE010000000
VERSION     DAZFRE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.355"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>818
                     /gene="traU"
                     /locus_tag="ACHKUI_005360"
     CDS             <1..>818
                     /gene="traU"
                     /locus_tag="ACHKUI_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO6601327.1"
                     /translation="ASADSACEGRFVNPITDICWSCIFPLSLGSVKVSQGKVPDTANP
                     SMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCMVNLGFSLPAFGKTAQGTAK
                     KDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAYLSEIDPTWTDSSLTTI
                     LNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGSQGSMYPFNGWVSNESSPLQ
                     SSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWRYQMVNMYPDSG"
BASE COUNT          175 a          218 c          234 g          191 t
ORIGIN      
        1 gcgtctgcgg attctgcctg tgaggggcgt tttgtaaacc cgatcacgga tatctgctgg
       61 agctgtattt tcccgctctc gctgggcagt gtcaaagtca gtcagggtaa ggtccccgac
      121 acggcgaacc cgtcgatgcc cattcagatt tgtccggcac cgccgccgct gttcaggcgt
      181 atcgggctgg ccattggtta ctgggagccg atggcgctga cggacgtcac ccggtcaccg
      241 ggatgcatgg ttaacctggg cttcagcctg ccggcttttg gtaaaacggc acagggaacg
      301 gcgaaaaagg atgagaagca ggtgaacggg gcgttctatc acgttcactg gtacaaatac
      361 ccgctgacgt actggctgaa catcatcaca tcgctgggct gtctggaagg tggtgacctg
      421 gatatcgctt atctttctga aattgacccc acctggacgg acagcagcct gaccaccatt
      481 ctcaatccgg aagctgtcat ctttgccaat ccgatagcac agggggcctg tgcagcagat
      541 gccattgcca gcgcctttaa tatgcctctc gatgttctgt tctggtgtgc cggttcgcag
      601 ggaagtatgt acccgttcaa tggctgggtg agtaatgagt ccagtccgtt gcagtcctcc
      661 ctgctggtca gtgaacgcat ggcgttcaaa ctacaccgtc agggcatgat tatggaaacc
      721 atcgggaaaa ataacgccgt ctgtaatgaa tatccgtccc caatcctgcc caaagaacgc
      781 tggcgttatc agatggtgaa tatgtatccg gacagcgg
//
LOCUS       DAZFRE010000350          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFRE010000350 DAZFRE010000000
VERSION     DAZFRE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.356"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            213..803
                     /locus_tag="ACHKUI_005361"
     CDS             213..803
                     /locus_tag="ACHKUI_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6601328.1"
                     /translation="MSKKLGFVLSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIDGSAIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
BASE COUNT          247 a          163 c          161 g          247 t
ORIGIN      
        1 taattttact aatttttatg tttagtaaaa ttaacttcca tatgtcatga tattatgcag
       61 aatgtctgtc caatgatgaa tgaatgttat acttggtaaa aaacaaacag acatcattaa
      121 caaagtaacg ttcagttaca tggcattgca tcaaaatctc atcatcatgc aatgctgtta
      181 tctgctttat ggaatattga taaaggattt ttatgtctaa aaaattaggt tttgtgctgt
      241 ccggtattat gttggcaatg gctgctggta ctgcatttgc tgctaacgat atggacggtg
      301 gtcagttaaa tattagcggc ctggtcgttg ataacacctg tgaagctcgt gttgatggcg
      361 gtaacaaaga cggtctgatc ctgctgcaaa ctgctaccgt tgctgaaatc accgatggtg
      421 tactggatac caccgtaggt gctaaagcta aaccattcag cattactatt gactgtagca
      481 aagctaaccc ggctccgggt actactgcaa aaatgacctt cggttctgtt ttctttggta
      541 acagcaaagg aacactgaac aacgacatgt ctattaacac cccgtctgac ggcgtaaata
      601 tcgctctgca caatattgat ggttctgcta ttaaacaggt tcaggtcaat aatccgggtg
      661 atgtttacag caaaactctg gattcaacgt ctaaatctgc aacctacgat tttaaagcat
      721 cctatgttcg tgcggatgca agcaaagcag ctactgccgg ttatgtaaaa accaactcag
      781 catataccat tacttaccag taaatatcaa ccgcttaa
//
LOCUS       DAZFRE010000351          805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFRE010000351 DAZFRE010000000
VERSION     DAZFRE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.357"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>805
                     /locus_tag="ACHKUI_005362"
     CDS             <1..>805
                     /locus_tag="ACHKUI_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6601329.1"
                     /translation="AIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGKSGAGKSSL
                     CNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRD
                     ILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGI
                     QPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLM
                     TRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT"
BASE COUNT          160 a          231 c          239 g          175 t
ORIGIN      
        1 aggcaattga aaaaccgctc tcctccctgc cttactcact ttcccgtcac atcctggaac
       61 atctgcgcaa actcaccagt cacgaacccg tgattggcat tatgggtaaa agcggggccg
      121 gtaaatcttc actctgtaat gcactgtttc agggggaggt cacaccggtc agtgatgttc
      181 acgccggcac ccgggaagtg cagcgcttcc gtctgagtgg tcacggtcac agcatggtta
      241 tcactgacct gcccggggtg ggcgagagcc gggacaggga tgcagagtat gaagccctgt
      301 accgtgacat tctgcctgaa ctggacctgg tgctgtggct gattaaagcc gatgaccggg
      361 ccctgtctgt ggatgagtat ttctggcgac acatcctgca gtgcggacat cagcaggtgc
      421 tgtttgtggt gacgcaggct gacaaaacgg aaccctgcca tgaatgggat atggcaggta
      481 ttcagccttc tcccgcacag gaacagaaca ttcgtgagaa aacggaggcg gtattccgtc
      541 tgttccggcc cgtacatccg gttgttgccg tatcggcccg caccggctgg gaactggata
      601 cgctggtcag tgcgctcatg acagcgcttc ccgaccatgc cgccagcccc ctgatgaccc
      661 gactgcagga cgagctgcgc acggagtctg tccgctctca ggcccgtgaa cagtttaccg
      721 gtgcggtgga ccggatattt gacacagcgg agagcgtctg tgttgcctct gttgtccgta
      781 cggccctgcg cgctgttcgt gacac
//
LOCUS       DAZFRE010000352          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFRE010000352 DAZFRE010000000
VERSION     DAZFRE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.358"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            110..184
                     /gene="tap"
                     /locus_tag="ACHKUI_005363"
     CDS             110..184
                     /gene="tap"
                     /locus_tag="ACHKUI_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6601330.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            177..>791
                     /gene="repA"
                     /locus_tag="ACHKUI_005364"
     CDS             177..>791
                     /gene="repA"
                     /locus_tag="ACHKUI_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6601331.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERF"
BASE COUNT          181 a          215 c          219 g          176 t
ORIGIN      
        1 aaaagtgaaa accccgataa tcttcgacaa gtttggcgac tgagaagatt accggggcca
       61 tctaaaaacc gtatagccaa caattcagct atgcggggag tatagttata tgcccggaaa
      121 agttcaagac ttctttctgt gctcactcct tctgcgcatt gtaagtgcag gatggtgtga
      181 ctgatcttca acaaacgtat taccgccagg taaagaaccc gaatccggtg tttacacccc
      241 gtaaaggtgc cggaacgctg aagttctgcg aaaaactgat ggaaaaggcg gtgggcttca
      301 cctcccgttt tgatttcgcc attcatgtgg cgcatgcccg ttcgcgtggc ctgcgtcggc
      361 gcatgccacc ggtgctgcgt cgacgggcta ttgatgcgct gctacagggg ctgtgtttcc
      421 actatgaccc gctggccaac cgcgtccagt gttccatcac cacactggcc attgagtgcg
      481 gactggcgac agagtccggt gcaggaaaac tctccatcac ccgagccacc cgggccctga
      541 cgttcctgtc agagctggga ctgattacct accagacgga atatgacccg cttatcgggt
      601 gctacattcc gaccgatatc acgttcacac cggcgctgtt tgccgctctc gatatctcag
      661 aagaggcggt ggcttccgca cgccgcagcc gtgtggaatg ggaaaacaga cagcgcaaaa
      721 agcaggggct ggataccctg ggcatggatg agctgatagc gaaagcctgg cgttttgtgc
      781 gtgagcgttt c
//
LOCUS       DAZFRE010000353          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFRE010000353 DAZFRE010000000
VERSION     DAZFRE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.359"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            109..183
                     /gene="tap"
                     /locus_tag="ACHKUI_005365"
     CDS             109..183
                     /gene="tap"
                     /locus_tag="ACHKUI_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6601332.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            176..>790
                     /gene="repA"
                     /locus_tag="ACHKUI_005366"
     CDS             176..>790
                     /gene="repA"
                     /locus_tag="ACHKUI_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6601333.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERF"
BASE COUNT          178 a          209 c          218 g          185 t
ORIGIN      
        1 aaaagcaaaa accccgataa tctttctatg tttggcgacg aagaagatta ccggggctaa
       61 caagaaaccg tatagccaac aattcagcta tgcggggagt atagtcatat gcccggaaaa
      121 gttcaagact tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac
      181 tgatcttcaa caaacgtatt accgccaggt aaagaacccg aatccggtgt ttacaccccg
      241 taaaggtgcc ggaacgttga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac
      301 ctcccgtttt gatttcgcca ttcatgtggc gcatgcccgt tcccgtggtt tgcgtcggcg
      361 catgccaccg gtgctgcgtc gacgggctat tgatgcgctg ctgcagggac tctgttttca
      421 ctatgatccg ctggccaacc gcgtccagtg ctccatcacc acgctggcca ttgagtgcgg
      481 actggcgaca gagtccggtg caggaaaact ctccatcacc cgtgccaccc gggccctgac
      541 gttcctgtca gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg
      601 ctacattccg accgacatca cgttcacacc ggctctgttt gctgcccttg atgtgtctga
      661 ggatgcagtg gcagctgcgc gccgcagtcg tgttgaatgg gaaaacaaac agcgcaaaaa
      721 gcaggggctg gataccctgg gtatggatga gctgatagcg aaagcctggc gttttgtgcg
      781 tgagcgtttc
//
LOCUS       DAZFRE010000354          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFRE010000354 DAZFRE010000000
VERSION     DAZFRE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.360"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          287 a          164 c          103 g          201 t
ORIGIN      
        1 gtaagatgat gaaattcaca tgaaaaatgc tgaagattat ttatttcatg cagataaacg
       61 ataaaaaaac aagagagtat atttttcagt gtaagagtca actgttcatc tccactgacg
      121 atcacggtca gcatacaaac aaagtgagtc ctcctgctta gtcattgcgt atcagaaact
      181 aaaaaacaaa agattctgca aacaacgtga cattttttat gacatttccc gaatacatgt
      241 atatcaataa acggtagtca tcgttgaatt aataagtctt acagagctat acgtagctta
      301 taaaaccact caacgaccat catcacctga ctatttagcg ggagtatcgt ctgtataaat
      361 ccataaataa taacacctgt tattaaaata agaatgctgt cagccgtaaa ctattaacac
      421 aacacctgtc cactctatct gttcttttaa ctcaaccggc gtcgaaaata tttcctgatt
      481 agtgcatcat agtcttcttt tttctttctc aaccatcaaa ccaaacacaa cattcacaac
      541 cacacaataa aacaaaacca aaacataaga ttaacaatgc cagtcaacag gaaaacactg
      601 aatattgagg tgcgctgtaa cctggacagc gcatacggcg catgtgatca acacagccca
      661 ttcttttctg tcatccccca aacgcagaac aaacaatcat aaccaaatga atataaaaga
      721 aaatatgatt tttcaccatc ataaaaacat caatg
//
LOCUS       DAZFRE010000355          753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFRE010000355 DAZFRE010000000
VERSION     DAZFRE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.361"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            65..>753
                     /locus_tag="ACHKUI_005367"
     CDS             65..>753
                     /locus_tag="ACHKUI_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6601334.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          252 a          127 c          166 g          208 t
ORIGIN      
        1 gctgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
       61 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
      121 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
      181 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
      241 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggtggag atgaaacgtt
      301 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
      361 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
      421 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
      481 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
      541 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctactg accttcataa
      601 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
      661 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
      721 tgatgccctt gaagatgacg acgatgtaca aaa
//
LOCUS       DAZFRE010000356          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFRE010000356 DAZFRE010000000
VERSION     DAZFRE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.362"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..738
                     /locus_tag="ACHKUI_005368"
     CDS             <1..738
                     /locus_tag="ACHKUI_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO6601335.1"
                     /translation="DPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVC
                     KGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHE
                     ANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSH
                     RKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEM
                     QKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          239 a          139 c          141 g          219 t
ORIGIN      
        1 gacccaaaat actttgctga tctgccgacg tcacagtttt ggtcgtacgc gacatacttc
       61 agggtattgt cctttgaata tcttagtgaa agtatttcca cactgctgta tctggatgcc
      121 gatgtcgttt gtaaaggaag tctgaaacct ctcacaaaaa ttatatttaa agatgagttt
      181 gctgcggtta ttcctgacaa tgatagtact caggcggcat gtgcaaaacg tcttaacatt
      241 cccgaaatga atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg
      301 catgaagcaa atttgacacc gtatttactc acgcttttac gaggggaaac taaatatggc
      361 tctcttaaat atttagatca ggatgcgttg aatatcgcat ttaatatgaa taatatctac
      421 ctcgcgaagg attttgatac tatttatacc ctgaaaaacg aacttcatga tcgtagtcat
      481 cgaaagtatc agcaaaccat taccgataaa acagtattga ttcactatac agggataact
      541 aaaccatggc atagctgggc tggatatccg tctgcatcat actttaatat cgcgcgtgaa
      601 caatctccct ggaagaaata tcctcttaaa gaggcgcgga ctgttgcaga aatgcagaaa
      661 caatataagc atctgtttgc ccatggtgag tatattaaag gtataacttc attaattaag
      721 tacaagctta agaaataa
//
LOCUS       DAZFRE010000357          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFRE010000357 DAZFRE010000000
VERSION     DAZFRE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.363"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(8..562)
                     /locus_tag="ACHKUI_005369"
     CDS             complement(8..562)
                     /locus_tag="ACHKUI_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6601336.1"
                     /translation="MKKIAMIVAMLTVSASGFAASGDVIGTGNQNVTGSLNSSTCAVS
                     FPSDVTLPNITTADYASASQYGLIGNASVSAGNISFNGCTGQNVKVVINSPNNKSSNG
                     IMAFPTLNGSNQSVVGIVLKEGISNKYVRVDGQESSNLMFTVDQDSFAIPVQVEALRA
                     SGSSMNSATSGTLSAQFTYTATYQ"
BASE COUNT          251 a          152 c          116 g          213 t
ORIGIN      
        1 aaacatttta ttgatatgtc gcagtgtatg tgaactgagc cgaaagtgta cctgatgtag
       61 cagaattcat ggaactacca ctggcacgta atgcttctac ctgtacagga atagcaaaac
      121 tatcttgatc gaccgtgaac attaagttcg aactttcctg tccatcaaca cgaacatatt
      181 tattactaat accttctttt aaaacaatac caactacaga ttggttcgaa ccattcagag
      241 ttggaaaagc cattataccg ttagaagatt tattgtttgg gctattaata acaaccttta
      301 cattttgtcc ggtgcaacca ttaaaagaaa tattacccgc tgaaacagaa gcatttccaa
      361 ttaaaccata ctgacttgct gaagcataat ccgctgtagt aatgtttggc agcgttacat
      421 cacttggaaa agaaacagca caggtagagc tattaagaga gccagtgaca ttctgattcc
      481 cagtaccaat aacatctcct gatgctgcaa aacctgatgc agagaccgtc agcattgcaa
      541 caatcattgc gatcttcttc atatgatata atccttatta acaaaaacaa aaaacaaact
      601 ttctttccaa aggcatttca ttgccttgcc aaataataat cagaagaaat gtcgggcgta
      661 aacacaaaga atatttaatt atcgaaaact tatatttatt ttatagtttt acaactgcca
      721 tggtctcccc tc
//
LOCUS       DAZFRE010000358          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFRE010000358 DAZFRE010000000
VERSION     DAZFRE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.364"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            21..>725
                     /locus_tag="ACHKUI_005370"
     CDS             21..>725
                     /locus_tag="ACHKUI_005370"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6601337.1"
                     /translation="MYIKIPQASMRNEVWFREPSATWDDGITSFLVNYNFSGTSQSYD
                     GNKTGNTYGSFESQINYGAWRLKNFSNYVRSSYQKKFKSVSSYLERGFDFFQGGRLTI
                     GQTTTDGTILDSIPLEGITISSDDGMLKPMLAQYSPTIRGIANSPARVIVKQNGNIIY
                     EKNVPAGEFEFNDVTQVYNGDLKIEIVESNGTVREFTQSAAELPVLQRKGRFRYNIAL
                     GEYRSDYKEFTKEPRFI"
BASE COUNT          259 a          101 c          157 g          208 t
ORIGIN      
        1 tttctgctaa agataacgca atttatataa aaatccctca ggcgtcgatg agaaatgaag
       61 tttggtttcg ggaaccatca gcaacatggg atgatggaat aacttctttt cttgttaact
      121 ataacttttc agggacatca caaagttacg atgggaataa aacaggaaat acttatggta
      181 gctttgagag ccaaataaac tatggcgcat ggcggttaaa gaattttagt aattatgtga
      241 gaagtagtta tcaaaaaaaa tttaaatctg ttagctctta tttagaaaga ggttttgatt
      301 tctttcaggg aggtcggttg acgataggtc aaacgacaac agacggtacc atactggata
      361 gcattcctct tgaggggata acaatatcct cagatgatgg gatgttaaaa ccaatgcttg
      421 cgcagtacag tccgactatt cgtggaatag caaattcacc agcaagagta atagtgaaac
      481 agaatggcaa tataatatat gaaaaaaatg tgcctgcagg tgaatttgaa tttaatgatg
      541 ttactcaggt ctataatggt gatttaaaaa ttgaaattgt tgagtcaaac ggaacagtta
      601 gagaatttac tcagtcagct gcagaattac cagtcttaca aagaaaagga aggtttcgtt
      661 ataatatagc attgggtgaa tataggagtg attataagga attcacgaaa gaacctagat
      721 tcata
//
LOCUS       DAZFRE010000359          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFRE010000359 DAZFRE010000000
VERSION     DAZFRE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.365"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..249
                     /locus_tag="ACHKUI_005371"
     CDS             <1..249
                     /locus_tag="ACHKUI_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO6601338.1"
                     /translation="IFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGILAAA
                     FVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            242..>717
                     /gene="traF"
                     /locus_tag="ACHKUI_005372"
     CDS             242..>717
                     /gene="traF"
                     /locus_tag="ACHKUI_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6601339.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDG"
BASE COUNT          191 a          172 c          178 g          176 t
ORIGIN      
        1 atattcacgt cgaaccgttt tattgatttt cttatccggc ttttgattac agcgattgtc
       61 atttccccgg taattatctg gtcgtgggat acggtaaaag aaactacggc agacgggata
      121 cttgctgctg ctttcgttat cctttactcc ggtgtgcttc tgtttattct gtatttttgt
      181 ttttctgccc tgactgacct gcagaaacct gatgaaagaa aaagtgatga aaggaatgaa
      241 gatgaataaa gcattactgc cactgttact ctgctgcttt atttttccgg cgtcaggaaa
      301 agatgcaggc tggcagtggt ataacgagaa aataaatccg aaggaaaaag aaaataaacc
      361 agtacctgca gccccccgtc aggagccgga cattatgcag aaactgaccg cgctgcagac
      421 ggcaacaaag cgggcgctgt acgaagctat tctgtatccc ggcgtggata attttgtgaa
      481 atatttccgg ctgcagaatt actggactca gcaggccggg cttttcacca tgagcgccaa
      541 aaaagccatg ctggcgcatc ccgaactgga ctataacctg cagtacagcc attacaacgg
      601 cacggtccgg aaccagctgg cggcagacca ggcgcagcag cgacaggcca ttgcgaaact
      661 ggctgaacac tacggcatca tgttctttta ccgggggcag gaccccatcg acgggca
//
LOCUS       DAZFRE010000360          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFRE010000360 DAZFRE010000000
VERSION     DAZFRE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.366"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..249
                     /locus_tag="ACHKUI_005373"
     CDS             <1..249
                     /locus_tag="ACHKUI_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO6601340.1"
                     /translation="IFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGMLAAA
                     FVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            242..>717
                     /gene="traF"
                     /locus_tag="ACHKUI_005374"
     CDS             242..>717
                     /gene="traF"
                     /locus_tag="ACHKUI_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6601341.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDG"
BASE COUNT          188 a          171 c          180 g          178 t
ORIGIN      
        1 atattcactt ctaatcgtct tgttgatttt cttatccggc tttcgattac agcgattgtc
       61 atttccccgg taattatctg gtcgtgggat acggtaaaag aaaccacggc agacgggatg
      121 cttgccgctg ctttcgttat cctttactcc ggtgtgctcc tgtttattct gtatttttgt
      181 ttttctgccc tgactggcct gcagaaagat gatgaaagaa aaaatgatga aaggaatgaa
      241 gatgaataaa gcattactgc cactgttact ctgctgcttt atttttccgg cgtcagggaa
      301 agatgcaggc tggcagtggt ataacgagaa aataaatccg aaggaaaaag aaaataaacc
      361 tgtacctgca gccccccgtc aggagccgga tattatgcag aaactggccg cgctgcagac
      421 ggcaacaaag cgggcgctgt acgaagccat tctgtatccc ggcgtggata attttgttaa
      481 atatttccgg ctgcagaatt actggactca gcaggccggg cttttcacca tgagtgctaa
      541 aaaggccatg ctggcacatc ccgaactgga ctataacctg cagtacagcc attacaacgg
      601 cacggtacgg aaccagctgg cggcagacca ggcgcagcag cgacaggcca ttgcgaaact
      661 ggctgaacac tacggcatca tgttctttta ccgggggcag gaccccatcg acgggca
//
LOCUS       DAZFRE010000361          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFRE010000361 DAZFRE010000000
VERSION     DAZFRE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.367"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>716)
                     /gene="istA"
                     /locus_tag="ACHKUI_005375"
     CDS             complement(<1..>716)
                     /gene="istA"
                     /locus_tag="ACHKUI_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO6601342.1"
                     /translation="KSEPPKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIARE
                     IRDQGYRGGMTILRGFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFV
                     AVLGYSRMLYIEFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTG
                     QHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGIT
                     VDVETANRHGLRWLHDVANQR"
BASE COUNT          171 a          188 c          197 g          160 t
ORIGIN      
        1 cgttggttag cgacatcgtg cagccagcgc agaccgtggc ggttggctgt ttcaacatcg
       61 acagtgatcc ccatcgggcg caggcgagtc attagtggga tgtaaaaact gttacgggtg
      121 tactgcacca tccgttccac cttaccttta gtctgtgccc tgaaggggcg acacagtcgg
      181 ggagagaagc ccatctcctt gccgaactgc cacagcgaag gatggaaccg gtgctgaccg
      241 gtctgatatg cgtcacgttg cagaaccaca gttttcatat tgtcatacaa cacttcgcgc
      301 ggcacaccac caaagaagcg gaacgcatta cgatggcagg tttccagcgt gtcataacgc
      361 atattgtcag tgaattcgat gtacaacatt cggctgtatc cgagaacagc aacgaacacg
      421 tgaagcggtg agcggccatt acgcatagtg ccccagtcaa cctgcatctg tcgtccgggt
      481 tcagtttcga accgaacggc aggctcctgc tcctgaggaa ccgagagaga acgaatgaat
      541 cccctgagaa tggtcattcc gccacgatat ccctggtctc tgatctcgcg agcgattacc
      601 gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt gacgaatata atcccggtat
      661 tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt ttggcggctc agattt
//
LOCUS       DAZFRE010000362          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFRE010000362 DAZFRE010000000
VERSION     DAZFRE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.368"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..640)
                     /locus_tag="ACHKUI_005376"
     CDS             complement(<1..640)
                     /locus_tag="ACHKUI_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFO6601343.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTL"
BASE COUNT          151 a          174 c          207 g          145 t
ORIGIN      
        1 ttaacgtcag agtgttatcg ccggtttgtt ctataagttg ctcagcaagc tcattttcgc
       61 tgttcttcat cgtgccgaag gctgcagccg tcagcagatg gctggtcagt tccatctggc
      121 agaccatttt gacctgcggg tagagcgccg ggttcccggc atgtgtctgg cgggggaagg
      181 ctgcatcgtt ctctggtgta tccggtgtgc gccagaacac accatcgatg gccagcaggg
      241 tcaggccgca ccagtgcgga tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga
      301 acacgcggcg gacagcctca cttcccaggc gctggcgggc ctgaataacg gcactggggg
      361 caacgaaggg gcgattgccc ggcagcatga tgtccaggcg attcacaatc tggtgaagag
      421 gttctttacg ctcaagcgcc atgccaacaa tacaccagac catcatttcg aggggaagac
      481 ggcgcttgcg tagcgttaca gtacctgatt cggcaaggca acgagagatg agttcggggt
      541 cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
      601 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      661 acacagccac tggatcg
//
LOCUS       DAZFRE010000363          674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFRE010000363 DAZFRE010000000
VERSION     DAZFRE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.369"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>674
                     /gene="kfiC"
                     /locus_tag="ACHKUI_005377"
     CDS             <1..>674
                     /gene="kfiC"
                     /locus_tag="ACHKUI_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267195202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis
                     glycosyltransferase KfiC"
                     /protein_id="HFO6601344.1"
                     /translation="NQTYENIEILVCDDCSSDKSLEIIKSIAYSDSRVKVYSSRKNQG
                     PYNIRNELIKKAHGNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGK
                     IQFFYDDKATRMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLL
                     QPLILGLWGDSGLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLL
                     SRYGLF"
BASE COUNT          237 a           98 c          147 g          192 t
ORIGIN      
        1 gaatcaaaca tatgaaaata ttgaaattct cgtgtgcgat gattgttcat cggacaaaag
       61 ccttgaaata attaagagca tagcttattc tgattcaaga gtgaaagtat atagctcacg
      121 aaaaaaccaa ggcccttata atataagaaa tgagctaata aaaaaagcac acggtaattt
      181 catcaccttt caagatgcag atgatctttc tcatccggag agaatacaaa gacaagttga
      241 ggttcttcgc aataataagg ctgtaatctg tatggctaac tggatccgtg ttgcgtcaaa
      301 tggaaaaatt caattcttct atgatgataa agccacaaga atgtctgttg tatcgtcaat
      361 gataaaaaaa gatatttttg cgacagttgg tggctataga caatctttaa ttggtgcaga
      421 tacggagttt tatgaaacag taataatgcg ttatgggcga gaaagtattg taagattact
      481 gcagccattg atattggggt tatggggaga ctccggactt accaggaata aaggaacaga
      541 agctctacct gatggatata tatcacaatc tcgaagagaa tatagtgata tcgcggcaag
      601 acaacgagtg ttagggaaaa gtatcgtaag tgataaagat gtacgtggtt tattatctcg
      661 ctatggtttg ttta
//
LOCUS       DAZFRE010000364          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFRE010000364 DAZFRE010000000
VERSION     DAZFRE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.370"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            20..>671
                     /locus_tag="ACHKUI_005378"
     CDS             20..>671
                     /locus_tag="ACHKUI_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601345.1"
                     /translation="MSKRNDITDGIFATTKKYGLVYTEELGWIDLGHAQGQDARILKR
                     KLEQEHFSTYYDEFHDWYFPVDYHQEMGIRKKILGVDLTFHTGVYTKVMVRSCLSPTL
                     KARVALTLMYGTAKRFEAWQNSFIFNWYTDSGFSAEDLVSDLIGFYRVFGTGPDPLLL
                     AKPLSYTKALQIWDTYGAPGNFKNTEFTPFLFTTHPPFKKNQLIKKKLPEWLNYIKP"
BASE COUNT          225 a          101 c          134 g          211 t
ORIGIN      
        1 tttttgaggg gagattgtga tgagcaaacg taacgatatt actgatggaa tatttgcaac
       61 aacgaaaaag tatggattag tttataccga agagttaggt tggatcgatt tggggcatgc
      121 tcagggacaa gatgccagaa tattaaaaag aaaattggag caagaacatt tttcaacata
      181 ttacgatgaa tttcatgatt ggtattttcc tgttgattat catcaggaaa tgggaattcg
      241 gaaaaaaata ttaggagtag atcttacatt tcataccggt gtttatacga aggttatggt
      301 tcgttcatgt ctttcaccca ctcttaaagc aagagttgcc ttaacgttaa tgtatggaac
      361 tgcgaagagg tttgaagcat ggcaaaactc atttattttt aactggtata ctgatagcgg
      421 atttagtgca gaagatttag tctcagactt gattggtttt tatcgtgtat ttggaacagg
      481 ccccgatcca cttttattgg caaaaccttt atcttacaca aaagccttac aaatatggga
      541 tacatatgga gcgccaggga attttaaaaa tactgagttt acgccattct tatttacaac
      601 ccatcctcct tttaaaaaaa atcaactaat aaaaaagaag cttccagaat ggttaaatta
      661 tataaagcca t
//
LOCUS       DAZFRE010000365          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFRE010000365 DAZFRE010000000
VERSION     DAZFRE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.371"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..402
                     /gene="istB"
                     /locus_tag="ACHKUI_005379"
     CDS             <1..402
                     /gene="istB"
                     /locus_tag="ACHKUI_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO6601346.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLII
                     DEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRI
                     LHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            470..660
                     /locus_tag="ACHKUI_005380"
                     /pseudo
     CDS             470..660
                     /locus_tag="ACHKUI_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          176 a          168 c          181 g          135 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatct
       61 acggcacaac gtcagggccg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaat aggctatctg ccgttcagtc aggaagaagc aaagctgttc
      181 ttccaggtca tcgctaaacg ttacgaaaag agcgcaatga tcctgacatc caatctgccg
      241 ttcgggcagt gggatcaaac gttcgccggt gatgcagcac tgacctcagc gatgctggac
      301 cgtatcttac accactcaca tgtcgttcaa atcaaaggag aaagctatcg actcagacag
      361 aaacgaaagg ccggggttat agcagaagct aatcctgagt aaaacggtgg atcaatattg
      421 ggccgttggt ggagatataa gtggatcact tttcatccgt cgttgacagg atgcatacgc
      481 cgggttcaac gagctgtacc gggatggccg gataacggaa gccgcctgtt gggctcacgc
      541 ccgccgtaaa atccacgatg tgcacgttcg caccccgtca gccctgacgg aggaagcgct
      601 gaaacggatc ggcgaactgt acgccatcga ggcagagata aggggaatga cggcggagca
//
LOCUS       DAZFRE010000366          652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFRE010000366 DAZFRE010000000
VERSION     DAZFRE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.372"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            110..445
                     /locus_tag="ACHKUI_005381"
     CDS             110..445
                     /locus_tag="ACHKUI_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO6601347.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
BASE COUNT          212 a          120 c          111 g          209 t
ORIGIN      
        1 ggtaaagaga atctgcgttt acttatcatg ttaaactccg gttattattt acatgagagt
       61 attcatgtct gtaatattcg cgtttgtgtg atattaatca gaggtacaaa tgaaaaagtt
      121 atcttttaaa gaaaaactgg aaatagtgag aaacatcata agagagtcat tatcaggtaa
      181 tgcggctttt gctttactgg tgtattcatt attgcactcc ctccccatta aaacctttgc
      241 tgatttattt gtgacgatac tgttatttat agcgattatc gctgttgtga tatggctatt
      301 tctaatcatc tatgtttatt tttgtgagtt attcagaagt cactggatag cagtctggtt
      361 tatcctctgg tctgtggtaa tcaacctcac tattggattt ggcatttatg acaaattagc
      421 accagtcatt tcactaatga aataataaaa aaccacatca ctccagacca tatggctgac
      481 aacataatac aaacaaacat ccttctgaaa ctgtcaaact tcgtgataac cggtatggta
      541 ctgactgcca ttgaaacaac cggtacggca cactgattca taaccgcacc acaaagaaac
      601 aagaaattat ctggcagcat acatacactt atgacattta ctacaaagaa ga
//
LOCUS       DAZFRE010000367          652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFRE010000367 DAZFRE010000000
VERSION     DAZFRE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.373"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(184..519)
                     /locus_tag="ACHKUI_005382"
     CDS             complement(184..519)
                     /locus_tag="ACHKUI_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO6601348.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFTDTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
BASE COUNT          229 a          138 c           99 g          186 t
ORIGIN      
        1 tatcattaca atatgtgcat tatcatataa tcttacagcg caagcttccc cgtcatacag
       61 acctcgtgat cgagttaata agactatgat agaactacaa actaacggag tagaacggca
      121 acttttagat aattcttgtg gcataagttc tggggtattt aaataaaata taagacaggc
      181 tgtctatctt acagacagcc tttttataat aacagaaaga atatatatca tacttaacgg
      241 aagagataat atgaaaacag tacccgtata aacagcaagc aatcccattt ttccaggtgt
      301 atcagtaaaa aaaccgctgt tccagaaatc aggccgggta aatttcagag ctgtatcttc
      361 aatataccat tttgcaaccg gatacaaaac cattccgcag agagaaatac accagaacag
      421 taatctgtat ttaaaatcat aatcccatga catatacagc atatatcccc ccgtcaccca
      481 tccccaccac atattattaa aataatattt tctgttcatc tgccagtttt cctttctttt
      541 gcacttttgt cagtgtactg atgcatgaca acataaccac agcatgataa taataatcaa
      601 taacaataag ctgtgtcacg tttacatcat ggaacggagg tgctcctgtg ac
//
LOCUS       DAZFRE010000368          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFRE010000368 DAZFRE010000000
VERSION     DAZFRE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.374"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(104..>642)
                     /locus_tag="ACHKUI_005383"
     CDS             complement(104..>642)
                     /locus_tag="ACHKUI_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601349.1"
                     /translation="PANIHESPYSPLCSGSFLIVTNNIRLDYIRKITCRFDLWMKKSD
                     WIEISPGEKLSYTVNLNDYYAFLPAKHQYDIGTVEFTLVNSNWFLEQHIYDLFFAIVD
                     ERHYKQCKNTSGIVRKYIYKSWQLCETYRGRWSPLQELMNRFHYEGGGENIFQIRTNQ
                     VNININGDKIKSLYSSMQ"
BASE COUNT          205 a          113 c           94 g          230 t
ORIGIN      
        1 gaattcttaa attctttcag tattgttatt aatgtttttg agaggaatgt gttttatatt
       61 ccgtgcatac gtgtttaata aatataccca caaaaaatta agctcactgc atcgatgaat
      121 aaagagattt aattttatct ccattaatgt ttatatttac ttgatttgtt cttatttgaa
      181 atatattttc accccctcct tcataatgaa atcgattcat gagttcctgt aaagggctcc
      241 acctcccacg ataagtctca cataactgcc acgacttata gatatacttc ctaacaatgc
      301 cagaagtgtt tttacactgc ttatagtgtc tttcatccac aatagcaaaa aaaagatcat
      361 aaatatgctg ctctaagaac caattactat ttaccaatgt gaactctaca gttcctatat
      421 catattgatg cttcgcaggg agaaaagcat aatagtcatt taaatttact gtgtatgaga
      481 gtttttctcc gggtgatatt tctatccagt cagatttttt catccacaaa tcaaatcggc
      541 atgtaatttt tcttatataa tctaaccgta tgttatttgt tacgattaaa aaagaaccac
      601 tgcacaaagg agagtaaggt gattcatgga tgtttgctgg ta
//
LOCUS       DAZFRE010000369          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFRE010000369 DAZFRE010000000
VERSION     DAZFRE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.375"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..630)
                     /locus_tag="ACHKUI_005384"
     CDS             complement(<1..630)
                     /locus_tag="ACHKUI_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO6601350.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS
                     FFGQYKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTIGYRPLLLQKIKTELLSSRLNKFNDFKDNFNGLGISIHDISAQKIS"
BASE COUNT          206 a           98 c          115 g          221 t
ORIGIN      
        1 agatattttt tgagctgaga tatcatgaat gctgattccc aggccattaa aattatcttt
       61 aaaatcattg aatttattta gtcttgatga aagtaattcg gtcttaatct tttgtaggag
      121 aagaggccgg tatcctatcg taggtgttga agaaatatca ttttcgatac attctttgat
      181 tacacgtatt gggctattat aatcatattt gtttattcgt tcatgaaaag ataagttcaa
      241 ttcctggcta tggaaactgt tgccatttcc atatctaaaa tgatcaatca gatcctggat
      301 gagtgttttg tattgtccaa aaaaggaaaa catttttgcc agatctttca tttctgcaaa
      361 taaaatatca atacattcac tttgtgatat ttttgttcca gaagttggaa cactaaaagc
      421 accataagta gaattcggcc caggaaaatg gtatttaatg aggcggtaag gatcaacttt
      481 tgcagagatg tcgcttaagc cgagtgagag aaaatcttgt tcttccatat ccccatactg
      541 catatcatca gcagaaaaat catcgaataa agtaatagtt tcgaaaagtg tacagggcat
      601 attaattgat gagcctaata tgttgttcat ttatattctc
//
LOCUS       DAZFRE010000370          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFRE010000370 DAZFRE010000000
VERSION     DAZFRE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.376"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          205 a          112 c          103 g          207 t
ORIGIN      
        1 tagtgatcgt aaacatggtt ctccagatta ctaaatttct ggcatcttac aatatcatca
       61 tcttcaaata gttaaattaa gattattgca ctgccaaaac agataaattg aagcagtctg
      121 gtagatattg cagaacaacc ttgattccaa cggcgaaatc gtatttatcc tccaggttgc
      181 cgtccatatg gagctgaata tcattcagtc cacgcttgat gagtcgatat cgctatatcc
      241 atagacaggg tagcttttta cacgagagtg tccggtgttt tctaacggct ggcaatcatt
      301 ccgttttgtt gtttcgtatc actgctataa caatttctct tgattttcaa tctttgagcg
      361 aatcattgaa tacagatcgg gaaatattaa taccatatat aatttatttt gaaaatcctt
      421 gttccatctg actataccta aggtatctat ttctttttgt gattattacc agtgaaacaa
      481 taagtgacgg atttacagta cgtcgctcat aaagacaaca atcaattaac gttgataaaa
      541 atatgttacg taaaatggca acaaagtagc tactctaact aattagtatt ataaaaagtc
      601 gataaagcgg ctctgttaaa taaaata
//
LOCUS       DAZFRE010000371          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFRE010000371 DAZFRE010000000
VERSION     DAZFRE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.377"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(176..484)
                     /locus_tag="ACHKUI_005385"
     CDS             complement(176..484)
                     /locus_tag="ACHKUI_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601351.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLYLSSDKFIPVKRRK
                     "
     gene            complement(511..>617)
                     /locus_tag="ACHKUI_005386"
     CDS             complement(511..>617)
                     /locus_tag="ACHKUI_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFO6601352.1"
                     /translation="FGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
BASE COUNT          200 a          137 c           92 g          188 t
ORIGIN      
        1 ggccattaca tcaaaagcac tcaccctttc cagcagggag aaaaaacgct cctcactgat
       61 aaaaatttct ttttctttat tacttatctt tcgtaaaaat gataaaaact cataacacac
      121 ctttatttat attatacata tacacacacg gttatattta tgagaaccga cagactcatt
      181 tcctgcgttt aacaggtata aacttatctg acgaaagata caaaacaaca acgcttacca
      241 ctgtcagaat aagaaccgtc ttcagataca gtttgacaag cagtattttt tccagtgtat
      301 ctgcatcctg ccagtcgtta acaatgtctg agataatatg ttcagggcaa agataaaacc
      361 acaccacagg gcatatcacc atagcaaaag gaaacagttt taatacattg cctgtcaaac
      421 gaatgaacac tcgggatata ctcatataca tatgaatgta tttactgctt aatttctttt
      481 ccatttaaaa tattcctctt tttgtttaag ttacaggaat acacagttac gtttacgcca
      541 catcaggtag ccgaagtttt tctttgtgtt gggcggattt tttcccgtct cccagcgcat
      601 cacactgcgc ccgaacg
//
LOCUS       DAZFRE010000372          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFRE010000372 DAZFRE010000000
VERSION     DAZFRE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.378"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..261)
                     /locus_tag="ACHKUI_005387"
     CDS             complement(<1..261)
                     /locus_tag="ACHKUI_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO6601353.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTADGVTVST"
BASE COUNT          157 a          141 c          158 g          147 t
ORIGIN      
        1 ggtactgaca gtcacgccat cagctgttcc ggatacaaac tggttgtcat ggtttaccag
       61 tgttccgcca ttcactgttt cacacgggtg cacaacgatg tcagcagcca gcaccgggag
      121 tgacgtgacc gcggcaagag acagtgccac cgccacaccg ccacgtttac cccgtgcgcg
      181 ggccagttcg gaggcaacca cgaaagcgcc cgtaatgtga ttccatacca gcctgtagca
      241 ggtattcaga tgtcgtttca tcagcatttc cttaatcaga gtgagggtga ataaaaaagc
      301 cggtatccac agatgtggat accggcaggt aaagggttac agtgccggag gtccggctga
      361 cggaagtttc tgacgtgtgg ctctgttgtt ccctgaatct catgtctgtg tgtatctgcg
      421 cacgttgcta acaggagtta ttattagcat aacgattgat attaacgatc ggtaaaattg
      481 gtattgatcg ttttattctt ttatcaagct attgcacgga caagagagac agtaacgaaa
      541 acagcgcttt tgtgagcata cttagaggca gggaggacat catccacaac aggttcagaa
      601 gaa
//
LOCUS       DAZFRE010000373          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFRE010000373 DAZFRE010000000
VERSION     DAZFRE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.379"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>603
                     /locus_tag="ACHKUI_005388"
     CDS             <1..>603
                     /locus_tag="ACHKUI_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO6601354.1"
                     /translation="YPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVSQGWN
                     NGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMSAGSQ
                     YNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKINDSWL
                     FAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPY"
BASE COUNT          201 a          107 c          123 g          172 t
ORIGIN      
        1 tatccactat taactgtaat tcttcttggt ttacctttgt gctctcaggc attaactctt
       61 aatctacgtg ggggatacag aagcgccagt cacgcttatg aaacgttgat tcaggttagt
      121 caggggtgga ataatggttg gtgggcaagt tttgagtcaa acacctggaa tacaattcac
      181 aacaatgaaa atgaattgtt tgcactcaat tataataaaa tagatataaa taaaacatat
      241 aaaatagatg acaaatgggc tctaatccca gggattcaga cacaatggat gtcagcagga
      301 agtcagtata acccttatct gaaaatttcg tatcaggcaa cgccagatct taatctggcc
      361 ttacgctatc gctatgactg gaaagctttc cgacagacgg atttagatgg caacagggcg
      421 cgtaatgatc agcatcagtt tgatggttat gtgacataca aaattaatga ttcatggttg
      481 tttgcatggc agaccacggt atatacgaag gttaataatt tcaaatatgg gaatcataaa
      541 aaaacagcaa ctgaaaatgc atttgttctg caatataaaa tgtcaccggt gttcacccca
      601 tat
//
LOCUS       DAZFRE010000374          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFRE010000374 DAZFRE010000000
VERSION     DAZFRE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.380"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            56..>586
                     /locus_tag="ACHKUI_005389"
     CDS             56..>586
                     /locus_tag="ACHKUI_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFO6601355.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYF"
BASE COUNT          195 a           85 c          132 g          174 t
ORIGIN      
        1 acatgaaaat agttaccgtg tcggtgttct gattaatttt taaaaggtga ttacaatgaa
       61 agtgaaaaac tttatatatc ttcccttctg cttgtttatt ggaacgtcgg tagccggagc
      121 tttaccggaa attcctgaac catttaaata tggtgtagga gggatcgaga atggtaaaat
      181 atatatagga ctaggaagtc tgggaaataa ctggtatatg atagatacaa accagagtga
      241 aaaaaaatgg actaagatag cgcaatggcc gacagttccc agagagcagg caacagctac
      301 tattattgat ggtaagatct atgtatttgg aggaattgga aaagataaaa gtggagtcat
      361 aaccttacaa aaagatgtct attcctatga tattgctaaa gataaatggg aaaaattgat
      421 gacccgtccc cctgtatctc ttgccggtca tgtttctttt atccataatg gtcatgcggt
      481 atctactggt ggtgtaaatg agaatatttt taatggatac ttttcagatg tcgagttatc
      541 aaaagggaat tccgcgctga cagagaaagt caacagggat tatttt
//
LOCUS       DAZFRE010000375          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFRE010000375 DAZFRE010000000
VERSION     DAZFRE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.381"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>582
                     /locus_tag="ACHKUI_005390"
     CDS             <1..>582
                     /locus_tag="ACHKUI_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFO6601356.1"
                     /translation="GLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSP
                     QARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIELGY
                     REIKQTMQRSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFS
                     ESCGMVMRMLMTLQGASPGRIPELMRDLASMGQL"
BASE COUNT          169 a          120 c          184 g          109 t
ORIGIN      
        1 gggactgtta aatgcctgga gcctggcggg agaacaccgc cactggatga tacctctcag
       61 aaagggagcg caatatgaag agatcagaaa actgggtaaa ggcgatcatc tggtgaagct
      121 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgactgcccg
      181 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      241 catgcgcttc cccggaggag aaatggggga tctgtacagt catcgctggg aaatcgaact
      301 gggatacagg gagataaaac agacgatgca acggagcagg ctgacgctga gaagtaaaaa
      361 gccggagctt gtggagcaag agctgtgggg tgtcttactg gcttataatc tggtgagata
      421 tcagatgatt aaaatggcgg aacatctgaa aggttactgg ccgaatcaac tgagtttctc
      481 agaatcatgc ggaatggtga tgagaatgct gatgacattg cagggcgctt caccgggacg
      541 tataccggag ctgatgcgcg atcttgcaag tatgggacaa ct
//
LOCUS       DAZFRE010000376          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFRE010000376 DAZFRE010000000
VERSION     DAZFRE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.382"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(65..370)
                     /gene="afaA"
                     /locus_tag="ACHKUI_005391"
     CDS             complement(65..370)
                     /gene="afaA"
                     /locus_tag="ACHKUI_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFA-III adhesin operon transcriptional regulator
                     AfaA"
                     /protein_id="HFO6601357.1"
                     /translation="MRERYLYLADTPQGILMSGQVPEYQFWLLAEISPVHSEKVINAL
                     RDYLVMGYNRMEACGRHGVSPGYFSGALKRFQRVSQTVYRLVPFYFPEAGHEVHRGE"
     gene            complement(367..>577)
                     /locus_tag="ACHKUI_005392"
     CDS             complement(367..>577)
                     /locus_tag="ACHKUI_005392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601358.1"
                     /translation="SDCILFLCYMILVFCDFVDKKIVQCIFLSKKHHDSICAVDAGGD
                     RYPVESEVKRFHSLHVIRVYGVNQR"
BASE COUNT          184 a          170 c           88 g          135 t
ORIGIN      
        1 agaaatagct ttaattagcg taataatatc aaccgcactt attaaacccg ctcacgccct
       61 ttcatcactc tcccctgtga acttcatgac ccgcctccgg gaaataaaaa ggcaccagcc
      121 tgtataccgt ctgactgacc cgctgaaacc gcttcagtgc accagaaaaa tatcccggcg
      181 acacaccatg acgcccgcag gcctccatac ggttatatcc cattaccaga taatccctca
      241 gcgcattaat aaccttctca ctgtgcaccg gcgatatctc agccagtaac cagaactgat
      301 attccggcac ctgaccggac atcagtatcc cctgaggggt gtcagcaaga tacagatatc
      361 gctccctcat ctctgattca ctccatacac acgaataaca tgcagggaat gaaacctttt
      421 cacttcagat tcaacaggat acctgtctcc ccctgcgtca acggcacaaa tactatcatg
      481 gtgtttctta cttaaaaata tacattgcac gatcttttta tctacaaaat cacaaaatac
      541 cagaatcata taacacaaaa aaagaataca atcagaa
//
LOCUS       DAZFRE010000377          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFRE010000377 DAZFRE010000000
VERSION     DAZFRE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.383"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>576
                     /locus_tag="ACHKUI_005393"
     CDS             <1..>576
                     /locus_tag="ACHKUI_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601359.1"
                     /translation="IKEILEIFINKNGGITKAKVKEVSLFSHGGPIHGPTTSDSVNTP
                     SVPKYPQQMDIIGGWDSIDFNWSNNAMFVMYGCRTSYASDDSGQGFASKLSLLDNFKD
                     VNVWGQTESTYPSYFPDIRTTSIMRSINIGWSFSPTYMVASSEGQGWDALFPDDKNPL
                     KSLPMQCYNNGKLILTCDQSSFNDHRKNKSND"
BASE COUNT          197 a           98 c          110 g          171 t
ORIGIN      
        1 atcaaagaaa tattagaaat ctttataaac aagaatggag gtataaccaa agcaaaagtt
       61 aaagaagtca gtttattctc acatgggggg ccaattcatg gtcctacaac ctcagactct
      121 gtaaatacac cttccgtgcc taaataccca caacaaatgg acataatcgg aggatgggat
      181 tcaattgatt ttaactggtc aaataacgct atgtttgtta tgtatggttg tcgcactagt
      241 tatgcaagtg atgattcagg acaaggtttt gccagtaaat tatccctatt agataatttt
      301 aaggacgtta atgtctgggg acaaacagaa agtacttatc catcttattt cccagatatt
      361 aggacaactt cgataatgag gtcaataaat ataggttggt ctttttcacc aacatacatg
      421 gttgccagtt ctgaaggtca aggatgggat gccttatttc cggatgataa aaatccacta
      481 aagtctctcc ctatgcagtg ttataataat gggaaattga ttttaacctg cgatcaaagc
      541 agttttaatg atcaccggaa gaataaatca aatgat
//
LOCUS       DAZFRE010000378          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFRE010000378 DAZFRE010000000
VERSION     DAZFRE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.384"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(141..343)
                     /locus_tag="ACHKUI_005394"
                     /pseudo
     CDS             complement(141..343)
                     /locus_tag="ACHKUI_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          174 a          116 c           97 g          189 t
ORIGIN      
        1 ttcataattg cctagttcaa agtatactgt ccatcctcct catacactaa atacaataga
       61 gtttatataa ttatatagag tgtgattaca atctccggca atgacactgt tagtgcaaca
      121 gtaatcaggc ctccctctta gtattgctta cgctgaaaga ataactggca gcctgccaca
      181 ccatgttgtc tggcaacgag gaagaccgcc attttcggtt caaggctctg ctgaaaaata
      241 gcgattttcc tgtgtggcac gctgtctgcg tttctctgct tctaagacac caattatcta
      301 ttctccaact actagtctaa aagactagta tcactactat cagttattta aatgatattg
      361 attgcctaga gattcaggga gctagtctag ttattcagag aacggtatct taacaatttt
      421 gttatgattt taggagatct ggatttctat tcatgatgct tggcgacaaa aataaatgaa
      481 caaaaagaac ttatatcagc cacagcaaca taatttctta tgtcacttgt attctacctg
      541 tgtcattaag tttatcgttt caatttcata agatac
//
LOCUS       DAZFRE010000379          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFRE010000379 DAZFRE010000000
VERSION     DAZFRE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.386"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          152 a          113 c          131 g          148 t
ORIGIN      
        1 tctgcccttt aataatctac ggacagatta ctttctgtac ttccagagcc tgtttctgtg
       61 agtcaaacat gagctggttt ttaccgacta cacccaagta aatatattcg atatctccca
      121 tttgccgtat tttcgtaaaa ttcaacagga aatattcaac gggagtgaga gaactgctgt
      181 ccggggagga ggtacagacg caaaaaaacc gactggtgag gccggttttt ttgcgattcc
      241 ggatcgaagt tggtagccct gaccggaaga taactgccta taactgacta tgaaataata
      301 cggctgaaat aatatttctg caagcacagt ataagtatat actcggtgtg tcagctttga
      361 ggcacgttct ttccggcata tcgttcaccg ggatgaacag gttgcaagtc ctggccaaaa
      421 catcaggtgg tgctggtttt ggtgctctgg ccggagaaga actgtctata actgacacta
      481 tagaaaacgt ggcgacaggc aatgtaaggg caaaccacgg tgttctttct ccattgatgg
      541 agaa
//
LOCUS       DAZFRE010000380          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFRE010000380 DAZFRE010000000
VERSION     DAZFRE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.387"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..214)
                     /locus_tag="ACHKUI_005395"
     CDS             complement(<1..214)
                     /locus_tag="ACHKUI_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6601360.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRV"
     gene            complement(315..524)
                     /locus_tag="ACHKUI_005396"
     CDS             complement(315..524)
                     /locus_tag="ACHKUI_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6601361.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRRW
                     RCRNSEADAGKQLNAWLASGGLLRQ"
BASE COUNT          125 a          140 c          148 g          130 t
ORIGIN      
        1 gcacacgggt ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta
       61 cggtgatggt ggggatgtac gtatagttct ggcttcggga cgtgtgttta tcttccccga
      121 aaatccccgg cacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct
      181 ggttcgcata accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga
      241 tttactggtg taataaatga aaaaagccgc gtctcccgga gaagacgcgg cctgatggat
      301 gaaatcaatg aggtttattg tctgagaagc cctccactgg cgagccacgc attaagctgt
      361 tttccggcat cagcttcaga gttccggcag cgccagcgtc gctgaccttc cgcgttgcgg
      421 ataacccgac ggaaatgcat tcccgggtca tcctggataa acacattgcg gtaagcggtt
      481 atgacagcaa ccgcctgccc gtatgagaaa gatccttcag ccaggacata ctctgtgtaa
      541 ccc
//
LOCUS       DAZFRE010000381          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFRE010000381 DAZFRE010000000
VERSION     DAZFRE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.388"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            81..329
                     /locus_tag="ACHKUI_005397"
     CDS             81..329
                     /locus_tag="ACHKUI_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6601362.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..>535
                     /locus_tag="ACHKUI_005398"
     CDS             307..>535
                     /locus_tag="ACHKUI_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6601363.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIIS"
BASE COUNT          162 a          125 c          133 g          115 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtg
//
LOCUS       DAZFRE010000382          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFRE010000382 DAZFRE010000000
VERSION     DAZFRE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.389"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..456
                     /locus_tag="ACHKUI_005399"
     CDS             <1..456
                     /locus_tag="ACHKUI_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6601364.1"
                     /translation="ARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAG
                     GSMALWGTALSAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYF
                     SSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          119 a          129 c          179 g          101 t
ORIGIN      
        1 gcccgtcttc acgaacagct gggtgaggga acggtcatcc acattgtgcc gcgactggcc
       61 ggggccggaa agggtggact gcagattgtg ctgggggcgg cagccatcgt gggctctttc
      121 ttcacggccg gaggctcgat ggcgttatgg ggtacagccc tgagtgccgg tggtttttct
      181 gccaccacga tgctgttttc actgggtgcc agcatgatac tgggcggagt ggcccagatg
      241 ctggccccga aggcaaaaac accggattac cgcgcaacgg ataacggcag acagaacacg
      301 tacttttcct cgctggataa catgattgcc caggggaacc cgatgccggt gccttacggg
      361 gaaatgctgg ttggctcccg ccgtatatcc caggacatca gtacccgtga tgaaggcggg
      421 ggcggaaagg tcgtggttat cgggcggcag gggtaaaaag aataaaaaaa tcccgcagtg
      481 atcgcggaca ggaactgcgg gagagttacg aagattaagt gtaaggaa
//
LOCUS       DAZFRE010000383          525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFRE010000383 DAZFRE010000000
VERSION     DAZFRE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.390"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            65..376
                     /gene="traL"
                     /locus_tag="ACHKUI_005400"
     CDS             65..376
                     /gene="traL"
                     /locus_tag="ACHKUI_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO6601365.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            398..>525
                     /locus_tag="ACHKUI_005401"
     CDS             398..>525
                     /locus_tag="ACHKUI_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO6601366.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQ"
BASE COUNT          132 a          110 c          126 g          157 t
ORIGIN      
        1 ccatactctc tgtatttatt gcagtaggta tgtctgttgc cggattctga caggaaataa
       61 aacgatgtcg ggagacgaga ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa
      121 ccagagccgc tggtttggcc tgccactgga tgaactgatc cccgcagcaa tctgtattgg
      181 ctggggtatc ataacatcga aatatctgtt cggtattggt gcagcggttc tggtttattt
      241 cgggattaaa aaactgaaaa aagggcgggg cagttcctgg ttacgtgacc tgatttactg
      301 gtatatgcca acagccctgc tgcgcggtat ttttcataat gttcccgatt cgtgtttccg
      361 gcagtggatt aaatagaact gataccagga ttgttatatg gaacacggtg cccgtttaag
      421 taccagtcgt gtaatggcca tcgcctttat atttatgtca gtgcttattg ttctcagcct
      481 ctctgttaac ctcattcagg gggtgaataa ctaccgtctt cagaa
//
LOCUS       DAZFRE010000384          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFRE010000384 DAZFRE010000000
VERSION     DAZFRE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.391"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(145..336)
                     /locus_tag="ACHKUI_005402"
     CDS             complement(145..336)
                     /locus_tag="ACHKUI_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6601367.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            354..506
                     /locus_tag="ACHKUI_005403"
     CDS             354..506
                     /locus_tag="ACHKUI_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601368.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMKVNLIIYND
                     DVIMMS"
BASE COUNT          168 a           91 c          126 g          137 t
ORIGIN      
        1 cgcagccgaa caggatgaac ttcagtcaga acaaagacgt aaaatttaag atgaaaaaat
       61 atctgccgct ttgataatcc caaagagcaa aagcatccga cagcgaggtg ccgttatgtt
      121 ctgtgagagt ggagaaggta accgctaacg ttgtaccggt gattcgacgg agagtgtgga
      181 ttgctgagct tcttttttgc tctgatggtg ctgccaggca ccgacggacg agtagataaa
      241 acggccaaag aagaagataa agctgatgag cagtacgata cgggtcatgc gactgttaaa
      301 tcggtgtcgt tttcgcatac tggttgcctg actcacaaaa ggttccttga agtatgtccc
      361 acgccgtgga cggtacttac attaaggcac aacaggacaa aatggtcaat tcttcgttat
      421 gtaaaaaagc gtcagttgat acatatttta atgttatgaa agttaattta attatttaca
      481 atgatgatgt aataatgatg agttaacatg ataaacgtaa ta
//
LOCUS       DAZFRE010000385          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFRE010000385 DAZFRE010000000
VERSION     DAZFRE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.392"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          180 a          115 c           88 g          138 t
ORIGIN      
        1 agttaaagct aagattcact agtataatga gtctgaacct ctgcatcaga gcaatcgcgg
       61 cacacacaag tgagtaaact agcgacacga taaaatccgt atcgactatc ataaccttcc
      121 gtacaccgct ggagttgaaa attatcagga aattcttaac gtacagaccc taccgaaagg
      181 actgtctgta taaaccagaa ttctatgctt atagaaaaag ctatgacata ctttgcaaaa
      241 gtcttaaata aattatggcc tagcatgaag gtgccaaggg aaccataaaa cttatcagtg
      301 atacgtaccg atcccctccg catagcctcg tccatacagc cccagacttt tagcctgact
      361 aagaaaatta tccgcattta tctgaataat gacatttaca tagtagtatg tatgtgagcc
      421 gctgttgtta cgcgaccatc tatgcgcaaa tgttttccat acaccgaatt tactacatga
      481 aaaaataaca ttcatcacaa ctatttagat tgataaagtg c
//
LOCUS       DAZFRE010000386          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFRE010000386 DAZFRE010000000
VERSION     DAZFRE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.393"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>515
                     /locus_tag="ACHKUI_005404"
     CDS             <1..>515
                     /locus_tag="ACHKUI_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601369.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGADARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAKPATI"
BASE COUNT          105 a          159 c          164 g           87 t
ORIGIN      
        1 accctgcctg atggtgtgga gacatacggg gtgtggggac tctccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggtt ccctgaacag cgtcatccca ccggcagtgc agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gcgaaatggg acacgccgcg ggtggtgaag
      301 ggtgtgcgct tcagtctgcg cctgaccagt ggtaagggag cggatgccag actggtgacc
      361 accgccatca ctgccgatac ggagcaccgt ttcagtggcc tgccactggg ggaatacacc
      421 ctgacggtca gggcgattaa cagttatggc cagcagggcg aaccggccac caccaccttc
      481 cggattaacg cgccagcaaa acccgccacc attga
//
LOCUS       DAZFRE010000387          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFRE010000387 DAZFRE010000000
VERSION     DAZFRE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.394"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..240
                     /locus_tag="ACHKUI_005405"
     CDS             <1..240
                     /locus_tag="ACHKUI_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601370.1"
                     /translation="VSQYPELIAQFSSGNQTRIKQGVIAKAPLEGWHYGTKEIVKKFH
                     MYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            241..>509
                     /locus_tag="ACHKUI_005406"
     CDS             241..>509
                     /locus_tag="ACHKUI_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO6601371.1"
                     /translation="MYNFKDEIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLP"
BASE COUNT          181 a           82 c          116 g          130 t
ORIGIN      
        1 gtgagtcagt atccggagtt gatagcgcag ttttcaagcg gtaatcaaac acgtataaaa
       61 caaggagtaa tcgcaaaagc accacttgaa ggttggcatt atggcactaa agaaatcgtt
      121 aaaaaattcc acatgtatca tcgtgttgcg attgaatata gtggcggaat ttacgatatt
      181 gataatttaa ggagggcaca cctcatttgc atgatgaaat ttattacagg agatagttaa
      241 atgtataatt ttaaagatga aatagaagat tacacagagc gggaattcat tgagttgctt
      301 ggcgagttta caaatccaac cggggataac gctcaattaa aaggggaaga gcttgacaaa
      361 tattgggatg atctagaaga acatcttacc agaattacac aacatccgtt gatgagcgat
      421 cttatctact atcctgcaaa gaagggagat gatgaacctg aaaacatcct gaaaattgtc
      481 aaagaatggc ggcgttcaca ggggctacc
//
LOCUS       DAZFRE010000388          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFRE010000388 DAZFRE010000000
VERSION     DAZFRE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.395"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>505
                     /gene="kfiA"
                     /locus_tag="ACHKUI_005407"
     CDS             <1..>505
                     /gene="kfiA"
                     /locus_tag="ACHKUI_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis alpha-UDP-GlcNAc
                     glycosyltransferase KfiA"
                     /protein_id="HFO6601372.1"
                     /translation="NDMIVLTDDDIIYPPDYVEKMLNFYNSFAIFNCIVGIHGCIYID
                     AFDGDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYMDGSQRFVDVRFSR
                     YMLENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAIAGYSKLNLE
                     LVYNVEG"
BASE COUNT          174 a           73 c           98 g          160 t
ORIGIN      
        1 aaaatgatat gatcgtactt acagatgatg atattattta ccctcccgat tatgtagaaa
       61 aaatgctcaa tttttataat tcctttgcaa tattcaattg cattgttggg attcatggct
      121 gtatatacat agatgcattt gatggagatc agtctaaaag aaaagtattt tcatttactc
      181 aagggctatt gcgaccgaga gttgtaaatc aattaggtac agggactgtt tttcttaagg
      241 cagatcaatt accatcttta aaatatatgg atggttctca acgattcgtc gatgttagat
      301 tttctcgcta tatgttagag aatgaaattg gtatgatatg tgttcccaga gaaaaaaact
      361 ggctaagaga ggtctcatca ggttcaatgg aaggactttg gaacacattt acaaaaaaat
      421 ggcctttaga catcataaaa gaaacacaag caatcgcagg atattcaaaa cttaacctcg
      481 aattagtgta taatgtggaa gggta
//
LOCUS       DAZFRE010000389          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFRE010000389 DAZFRE010000000
VERSION     DAZFRE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.396"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>503
                     /locus_tag="ACHKUI_005408"
     CDS             <1..>503
                     /locus_tag="ACHKUI_005408"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6601373.1"
                     /translation="IPDGTWFSKILFVRSFLKEHPHNILHSHGITADMFSYFLNGVKI
                     STIHNRLDEDYIPLFGAVKGNAIYYLHRFILRRFNHIVACSAAVQSKLKQSKVKTKIT
                     TIQNGIDITRFKTLESDKKKLLREKHGFDSEKRIFIYCGSLSLRKNIAYLLEHLAIEE
                     NDIFLIL"
BASE COUNT          181 a           75 c           85 g          162 t
ORIGIN      
        1 taataccaga tggtacttgg ttcagcaaaa ttttatttgt gagaagtttt ttaaaggaac
       61 atccacataa tatcttacat tcacatggga tcacggccga tatgttttct tactttctga
      121 atggcgtgaa aatatctact attcacaata gactagatga ggattatatc ccattatttg
      181 gcgcggttaa agggaatgct atatattatc ttcatcgttt tatattacga agatttaatc
      241 atatcgttgc ttgctcagca gcggtccaat caaaactgaa acaatcgaaa gtaaaaacta
      301 aaataaccac catccagaat gggattgata taactaggtt taagacactt gagtctgata
      361 aaaaaaaatt attgagggaa aaacacggat ttgatagtga aaaaagaata tttatatatt
      421 gtggctcgtt atcattaagg aaaaatattg cttacctctt ggaacactta gccatcgaag
      481 aaaatgatat atttttaatt cta
//
LOCUS       DAZFRE010000390          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFRE010000390 DAZFRE010000000
VERSION     DAZFRE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.397"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          162 a           96 c           80 g          152 t
ORIGIN      
        1 cagctttaaa ctgattttag ctaacgtaaa gatttactaa actggatgtg tatcagatca
       61 aacaatgtgt acagcaatgc ttaatgaaaa cacttgtaga attgtttatg aaaatgcgtg
      121 tataacactt agtgaagata tgtctgtata atacgtaata tagtaccatt cccgacattc
      181 tcaatatctt cagacatcca tgcagactga gtgttgattt cagtttttca cggccagcat
      241 ggatctttac tggtcaaaaa acagacatga ttactctgga attatttttc tgattccgtc
      301 ttcagaacaa caaaaactcg tgataactct acaacaaact atcgtaattg gcttctgtaa
      361 ccccgtcact tttcctaaga aacgtgcccc catatgatgc gggagagata agtcatcgta
      421 tacaggaaac actgccaatc ataatatggc gtcattcagt aatttttatc cataatcaga
      481 ttagcatatc
//
LOCUS       DAZFRE010000391          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFRE010000391 DAZFRE010000000
VERSION     DAZFRE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.398"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          152 a          156 c           84 g           95 t
ORIGIN      
        1 ttccgcacgt ctgaagcccg acagcgcaca aaaaatcagc accacataca aaaaacaacc
       61 tcatcatcca gcttctggtg catccggccc cccctgtttt cgatacaaaa cacgcctcac
      121 agacggggaa ttttgcttat tcacattaaa ctgcaaggga cttccccata aggttacaac
      181 cgttcatgtc ataaagcgcc atccgccagt cttacagggt gcaatgtatc ttttaaacac
      241 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctattcact
      301 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgga
      361 gccactttag ttacaacaca caaaaacaac ctccagaaaa accccggacc agcgcagaac
      421 cgaaaccaca aagccctgcc tcataactga aaagcggccc cgccccggcc caaagggccg
      481 gaacaga
//
LOCUS       DAZFRE010000392          486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFRE010000392 DAZFRE010000000
VERSION     DAZFRE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.399"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..126)
                     /locus_tag="ACHKUI_005409"
     CDS             complement(<1..126)
                     /locus_tag="ACHKUI_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO6601374.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLE"
     gene            complement(99..218)
                     /locus_tag="ACHKUI_005410"
                     /pseudo
     CDS             complement(99..218)
                     /locus_tag="ACHKUI_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(237..>486)
                     /locus_tag="ACHKUI_005411"
     CDS             complement(237..>486)
                     /locus_tag="ACHKUI_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601375.1"
                     /translation="QVLAGLFTGNWAYGAIAGCTFFIAREHTQAEYRWIEMFGHGKRI
                     NMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
BASE COUNT          127 a          154 c           96 g          109 t
ORIGIN      
        1 ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca accgcagcac
       61 gttcttgctt aagacgtgct gcggcataat cccaatgatt actccctgac agggttcgta
      121 ggccactcaa tatcaggtgc agttgatgta tcaacacggt tcagcaacac cctgcatgcc
      181 ttccatgccg ccagcaatga tgtttcctcc ttcgttgcat atactgctca cctttttcac
      241 ccacgattaa ccaacagcca gaccagcaga cacgccacca ccggcacagc aaaatccatc
      301 aggcttgcca catcccatgc acgtggatca aaaccgcccc accacggcat gttaatccgc
      361 ttgccatgcc cgaacatttc aatccagcga tattctgcct gggtgtgttc acgcgcaatg
      421 aagaacgtac aaccggctat cgccccgtaa gcccagtttc cggtaaaaag accagccagt
      481 acctgc
//
LOCUS       DAZFRE010000393          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFRE010000393 DAZFRE010000000
VERSION     DAZFRE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.400"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..217
                     /locus_tag="ACHKUI_005412"
     CDS             <1..217
                     /locus_tag="ACHKUI_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601376.1"
                     /translation="GLRNGVVPYIPAAFCRDAGSAGHGATEAARPERSLQAGGFHASD
                     RSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            complement(157..381)
                     /locus_tag="ACHKUI_005413"
     CDS             complement(157..381)
                     /locus_tag="ACHKUI_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601377.1"
                     /translation="MSVTDDVVVPATATATATATATALLFPAALILTTAVLSCLPGAP
                     SAPGCLAASGRQARLRQAAAGAPASLRRTR"
     gene            complement(291..>480)
                     /locus_tag="ACHKUI_005414"
     CDS             complement(291..>480)
                     /locus_tag="ACHKUI_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601378.1"
                     /translation="PRAGGAERGCAHAPPLSLRGRSDGGFCLFRPVICLLLMMLLFLL
                     LLLLLLLLLLLLFFFLPL"
BASE COUNT          115 a          138 c          168 g           59 t
ORIGIN      
        1 cggtttgcga aacggcgttg tgccttacat cccggcggcc ttttgccggg atgccgggag
       61 cgcaggccac ggcgcgacag aggcggcgcg accggaacgg tcgctgcaag ccgggggttt
      121 tcacgcatcg gaccgtagcg acagcccgaa accgcgtcag cgggttcggc ggagactggc
      181 gggtgcgcca gcagctgcct gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc
      241 gggagccgaa ggtgcgcccg gcagacagga caacactgca gtagtcagaa tcagagcggc
      301 aggaaaaaga agagcagtag cagtagcagt agcagtagca gtagcagtag caggaacaac
      361 aacatcatca gtaacagaca aatgacaggt ctgaaaagac agaagccccc atcagaacgt
      421 ccgcgtaatg aaaggggcgg cgcgtgagcg cagccccttt cagcgccacc ggcgcgcggt
//
LOCUS       DAZFRE010000394          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFRE010000394 DAZFRE010000000
VERSION     DAZFRE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.401"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..314)
                     /locus_tag="ACHKUI_005415"
     CDS             complement(<1..314)
                     /locus_tag="ACHKUI_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO6601379.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SS"
BASE COUNT          121 a          104 c          120 g          114 t
ORIGIN      
        1 tgactggaca tcagatggat acacaacgga ataccgcgcg gcagtttggt gcgcagcatg
       61 tgatcgagtg cctccacaat ggacttattc gccccattga tgggaatggc ttccagcata
      121 aagcccatcg tggtgtcatt catgaacaga ccggattcct ggttgtaatc acggtacggc
      181 agcagacggc tgaactgcgg gaagctcatt tcgcccagaa cttcattggc ctttgcggat
      241 tcgtcaggca gtttcagtgc tgtgacgagg gagttaaccg cctgagtgac ggcctcaagt
      301 gggttattac tcatatgagt attccttagt gcgtttaaaa aaggaaaata tcagtctgtg
      361 gccgtggata tatcacgcag tgccattcct gaaaccacag cacaaataag agatggaggc
      421 ttttaacagg aaaccaggat taacgttcct ttactgaat
//
LOCUS       DAZFRE010000395          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFRE010000395 DAZFRE010000000
VERSION     DAZFRE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.402"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..140)
                     /locus_tag="ACHKUI_005416"
     CDS             complement(<1..140)
                     /locus_tag="ACHKUI_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO6601380.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     RE"
     gene            complement(149..454)
                     /locus_tag="ACHKUI_005417"
     CDS             complement(149..454)
                     /locus_tag="ACHKUI_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601381.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVLVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
BASE COUNT          142 a           94 c           78 g          145 t
ORIGIN      
        1 ttctctcgca gttgtctgct taaattttcc tgctgcttca ggaactggcg gttttcaggt
       61 gtgttcacat tttctgaagc atgaacagca agatgcatcc ctgctgtcag agcaaaaagt
      121 gcaaatattt tccggttcat gatatttctc actttctgct atttacaggc atgaactcat
      181 cagaaaaaag ataattgaga aaaacagcca taaacgcaca tgtaaatgtc accttcagat
      241 ataatctgac aagtgtcact tgttctgata tatctgcctt cttccattca tgaataattt
      301 cagcgagaaa actgtcaggt gcaaaataaa ataatataac gagcaccatt acaagaccaa
      361 aaggaaataa ttttaatgtt ctgcttatca gcctgagcag cacagcactt attttcctga
      421 ggtgctgaat atatttactg tccattgttg ccattttca
//
LOCUS       DAZFRE010000396          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFRE010000396 DAZFRE010000000
VERSION     DAZFRE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.403"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>457
                     /locus_tag="ACHKUI_005418"
     rRNA            <1..>457
                     /locus_tag="ACHKUI_005418"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          127 a          101 c          147 g           82 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaaccaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgc
//
LOCUS       DAZFRE010000397          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFRE010000397 DAZFRE010000000
VERSION     DAZFRE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.404"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..268
                     /locus_tag="ACHKUI_005419"
                     /pseudo
     CDS             1..268
                     /locus_tag="ACHKUI_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            354..>451
                     /locus_tag="ACHKUI_005420"
     CDS             354..>451
                     /locus_tag="ACHKUI_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFO6601382.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
BASE COUNT          103 a          118 c          135 g           95 t
ORIGIN      
        1 ctgccggctg ctgtctccgc tggaagaggc gcttcatggc tatgtgctga ctgacggtaa
       61 gctccatgct gatgacacgc ctgtcccggt gctgttgcca ggcaataaga aaacgaagac
      121 cgggcggtta tggacctacg ttcgtgacga ccgtaacgcc gggtcaacgc tggcgccggc
      181 ggtgtggttc gcttacagcc cggacagaaa aggcatccat ccgcagaccc atcttgcggg
      241 gttcagtggt gtactgcagg cggatgctgt cacgaacggt gcaatagtgc tccacaccca
      301 acgcctgaaa tcagatccag ggggtaatct gctctcctga ttcaggagag tttatggtca
      361 cttttgagac agttatggaa attaaaatcc tgcacaagca gggaatgagt agccgggcga
      421 ttgccagaga actggggatc tcccgcaata c
//
LOCUS       DAZFRE010000398          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFRE010000398 DAZFRE010000000
VERSION     DAZFRE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.405"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            212..>433
                     /locus_tag="ACHKUI_005421"
     CDS             212..>433
                     /locus_tag="ACHKUI_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601383.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRH"
BASE COUNT          116 a           94 c           94 g          129 t
ORIGIN      
        1 attctctgaa cctcgtcagg aggctgatct gaccggcctc cttgctgacg cctcgcgttg
       61 ctcgttgtcc aaccccggga tgtaacaaaa taaattttgt cacctcccgg cgtttccgaa
      121 ataaatatga aaacactgag cggttgtgca aattctttgt gcgaccgtga agtgttgccg
      181 gcgaagccgg gatcttccgg agaaagtcat tatgacatca gacaatatcg ctgaatatgt
      241 tggtgagaat aatcaattta ctgagaagcc tgtaactgaa taccccccgg tgatacagga
      301 taatacagtg accactaaag actatctgta cagcaataat attgatactg accttttagt
      361 ggttatcgga gggcttattg ttatattgtt tctcatgatg cttatcatgt actttattaa
      421 ttctctcagg cat
//
LOCUS       DAZFRE010000399          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFRE010000399 DAZFRE010000000
VERSION     DAZFRE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.406"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..99)
                     /locus_tag="ACHKUI_005422"
     CDS             complement(<1..99)
                     /locus_tag="ACHKUI_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6601384.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTV"
     gene            complement(251..>424)
                     /locus_tag="ACHKUI_005423"
     CDS             complement(251..>424)
                     /locus_tag="ACHKUI_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6601385.1"
                     /translation="DVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDW
                     RTDAFIVGIGYRF"
BASE COUNT          107 a          121 c           93 g          103 t
ORIGIN      
        1 caccgtgctg ctggtccgtc tggccttcag cacaatggtg cagttctgta ctggttttcc
       61 tgtgccatct ttaagcacgc cagaaatttt tactgtcata cttttccacc aataaaaaaa
      121 gcccgcagca gtgacgccac gggcttcagg acagtgtaac tttacgtttc ctcaaacgca
      181 gttcacccca taaggcggat gaacctgcgt atcataacaa tatttacaga agataaatcg
      241 gcgtctgttg tcagaaacgg tatccgatac caacaataaa tgcatccgtt cgccagtcgc
      301 cactgccgga gccttcataa gcaatatcaa tggccacgga ttcggtcggg ttaaactgca
      361 cgccagctcc ccacgccaga gaggtattgc tgtggcgacc gtcatcactt ccggtcagca
      421 cgtc
//
LOCUS       DAZFRE010000400          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFRE010000400 DAZFRE010000000
VERSION     DAZFRE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.407"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          131 a           64 c           81 g          146 t
ORIGIN      
        1 tcttcatttc tgttaaaaaa acagagttta tatgctatta cggatttcag tcgtagcaag
       61 ttgaactgtg atcgcattaa gactaatctt ctggcttgta aaggccttag ttccgtcatg
      121 actctcccat caggccatag ttgagcaaaa tcctgaaaga attatcacct attgtatctt
      181 cagatacggg agtttttgtc cagtattgtt ggaataatca tgtgcctata ttgttcttgt
      241 agttggaacg tcttataaat aaaacgaaaa ggcagtaagt ttgttgtatt tattgatatt
      301 tttcattcag tcagttggca ataatcagtt atggttgtat tcaaaagtac agctagaacg
      361 aaattaaaat aacgggagca tcgtacatga cttgatattt cagagggcta agtattaata
      421 ta
//
LOCUS       DAZFRE010000401          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFRE010000401 DAZFRE010000000
VERSION     DAZFRE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.408"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>414
                     /locus_tag="ACHKUI_005424"
     CDS             <1..>414
                     /locus_tag="ACHKUI_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO6601386.1"
                     /translation="QAGAFNYTLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDR
                     ILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLL
                     RTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTV"
BASE COUNT           88 a          113 c          125 g           88 t
ORIGIN      
        1 tgcaggccgg ggcctttaac tacaccctca accgggacag tgatgagagc tggtatctgc
       61 gcagtgaaaa tgcttatcgt gctgaagtcc ccctgtatgc ctccatgctg acacaggcaa
      121 tggactatga ccggattctg gcaggctccc gcagccatca gaccggtgta aacggtgaaa
      181 ataacagcgt ccgtctcagc attcagggcg gccatctcgg tcacgataac aacggcggta
      241 ttgtccgtgg tgccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg
      301 acctgctcag aacagaggtt gccggtatgt cactgacgac aggagtgtat ggtgctgcag
      361 gccattcttc cgttgatgtt aaggatgatg acggctcccg tgccggcacg gtcc
//
LOCUS       DAZFRE010000402          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFRE010000402 DAZFRE010000000
VERSION     DAZFRE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.409"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>407)
                     /locus_tag="ACHKUI_005425"
     CDS             complement(<1..>407)
                     /locus_tag="ACHKUI_005425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO6601387.1"
                     /translation="FSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDAL
                     LQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELG
                     KAIDFDARTAIPFEGERHNALDDARYQAKYVSV"
BASE COUNT           99 a          104 c           94 g          110 t
ORIGIN      
        1 taactgaaac gtattttgcc tggtaacggg cgtcatcaag tgcattatgg cgctcacctt
       61 cgaatggaat agccgttctg gcatcgaagt ctatggcttt ccccagctca acgattgtgc
      121 gtacatcgcg atcgttgtag taacgccacg ggcaggggat cccctgccgt tcgtatgaac
      181 ggcgcaaaat cgtgttgtcg aagttggctc catttcccca gacctgaaca aaaaattcac
      241 cggagttttc gtcgataaat tcccgcaatt gtaacagtgc atcatctaac gggatttcat
      301 cggtcataat ggcagattgc gcttcgcgtg attgcttaag ccaccattta atggtgtccc
      361 gatcaatgac cccgccagca gtttccagat cgatagtctt actaaat
//
LOCUS       DAZFRE010000403          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFRE010000403 DAZFRE010000000
VERSION     DAZFRE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.410"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            3..404
                     /locus_tag="ACHKUI_005426"
     CDS             3..404
                     /locus_tag="ACHKUI_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6601388.1"
                     /translation="MAAKYSGDTPLMLVIWSPEEIQALSDGMDIALTDHEIRTVLARL
                     EDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQALWKREWAAWD
                     HGLAVPECVTRRQAVVNQVRILLKNNTHEND"
BASE COUNT           97 a          113 c          117 g           79 t
ORIGIN      
        1 agctggcagc aaagtattcg ggagacacgc cactgatgct ggttatctgg tccccggaag
       61 aaattcaggc cctctctgac ggaatggata ttgccctgac cgatcatgaa atcaggactg
      121 tcctggcgcg cctggaggac atcccggaag accagcggac tgaatccggt atttcttccg
      181 gcgtggcgat ggagatcatc aataacgtga gcgaaaaccg ccaggtgact gtccctgctg
      241 aactgctggc gtccctgatt cagaccgctg aacaggcatt gtggaaacgt gaatgggccg
      301 cctgggatca tggcctcgcc gtcccggaat gcgtcacccg ccgtcaggcg gtggttaatc
      361 aggtccgcat cctgctgaaa aacaacacac acgaaaacga ctgatg
//
LOCUS       DAZFRE010000404          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFRE010000404 DAZFRE010000000
VERSION     DAZFRE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.411"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>404
                     /locus_tag="ACHKUI_005427"
     CDS             <1..>404
                     /locus_tag="ACHKUI_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO6601389.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTG"
BASE COUNT           98 a           96 c          110 g          100 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctgg
//
LOCUS       DAZFRE010000405          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFRE010000405 DAZFRE010000000
VERSION     DAZFRE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.412"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            97..>402
                     /locus_tag="ACHKUI_005428"
     CDS             97..>402
                     /locus_tag="ACHKUI_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601390.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     "
BASE COUNT          144 a           66 c           87 g          105 t
ORIGIN      
        1 tgtattgaag ttaaaacaaa tgaacctttg aacagcgtga tggaaacact aaatggtggt
       61 gggcaatgag tgcttactgc ttcgaaggaa aatgaaatga agtcgatata tttaaaatct
      121 gttctggctt ttatatttgt tggcgttatg gctatgattg tatgtattcc tttttacatc
      181 gtttaccttg cgcaacaacc tgcaacacca gaacaactga cagagatttt acaggaaact
      241 ccctgtgcag cagaagcatt tcaggagaca cttaattatc agtctgaacc tttaacactt
      301 ggtaaggcaa ataaaatcgc atcagaatgc agaaaaagaa acgaaatggc agaggtgaaa
      361 cgggtaagag aaaatgagcg caataagatt cgcgaaaaac aa
//
LOCUS       DAZFRE010000406          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFRE010000406 DAZFRE010000000
VERSION     DAZFRE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.413"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>389
                     /gene="traN"
                     /locus_tag="ACHKUI_005429"
     CDS             <1..>389
                     /gene="traN"
                     /locus_tag="ACHKUI_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO6601391.1"
                     /translation="KTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAA
                     GKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAK
                     SNKLTVSVGEFCSKKVLGVCLQKKRSY"
BASE COUNT          100 a           80 c          129 g           80 t
ORIGIN      
        1 aaaacatccg ggaaagtgat ggtctgtggg ggagacgtct tctgtctcga tggcgaatgt
       61 gataaggctc agagcgggca gagtaatgat tttgccgaag ccgtttctca gctggcggca
      121 ctggccgcag caggtaagga tgttgcggcg ctgaacgggg tggatgtccg tgctttcacc
      181 ggtcaggcga aattttgtaa gaaggctgca gccggataca gcaactgctg taaggacagc
      241 ggctggggac aggatatcgg gctggccaaa tgcagcagtg atgaaaaggc cctggctaaa
      301 gcaaaatcaa acaaactgac cgtcagtgtc ggagagttct gttcgaagaa agtgctgggt
      361 gtctgtctgc agaaaaaacg cagctactg
//
LOCUS       DAZFRE010000407          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFRE010000407 DAZFRE010000000
VERSION     DAZFRE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.414"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>389
                     /gene="traN"
                     /locus_tag="ACHKUI_005430"
     CDS             <1..>389
                     /gene="traN"
                     /locus_tag="ACHKUI_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO6601392.1"
                     /translation="RTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAA
                     GKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAK
                     DNKLTVSVGEFCSRKVLGVCLQKKRSY"
BASE COUNT           95 a           75 c          139 g           80 t
ORIGIN      
        1 agaacatcag gaaaagtgat ggtctgtggt ggtgatgtct tctgccttga cggtgaatgc
       61 gataaggcac agagcggaaa aaacagtgat tttggtgaag cagtatcaca gctggctgcg
      121 cttgccgccg caggtaagga tgttgccgcc ctgaatggtg tggatgtcag ggctttcacg
      181 ggtgaggcga aattctgccg taaggccgca gccggcttca gtaactgctg taaggatggc
      241 ggctggggtc aggatgtggg gctggcgaag tgcaacagtg aagaaaaggc gctgggaaag
      301 gcaaaggaca acaaactgac ggtgagtgtg ggcgagttct gctcccggaa agtgctgggt
      361 gtctgtctgc agaaaaagcg cagctactg
//
LOCUS       DAZFRE010000408          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFRE010000408 DAZFRE010000000
VERSION     DAZFRE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.415"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>389
                     /locus_tag="ACHKUI_005431"
     CDS             <1..>389
                     /locus_tag="ACHKUI_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO6601393.1"
                     /translation="VLNGAIDPTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTS
                     ARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAG
                     NSGTGLATTGKGIQVVEAINGATTEEG"
BASE COUNT           95 a          110 c          116 g           68 t
ORIGIN      
        1 cagtgctgaa cggtgccatt gaccccacga atgtcaccct gacatccggt gccacctgga
       61 atatccccga taacgccacg gttcagtcgg tggtggatga cctcagccat gccggacaga
      121 ttcatttcac ctccgcccgc acagggaagt tcgttccgac aactctgcag gtgaaaaacc
      181 tgaacgggca gaatggcacc atcagcctgc gtgtacgccc ggatatggcg cagaacaatg
      241 ctgacagact ggtcattgac ggtggcaggg caaccggaaa aaccatcctg aatctggtga
      301 acgccggcaa cagtggtacg gggctggcga ccaccggtaa gggtattcag gtggttgagg
      361 ccattaacgg tgccaccacg gaggaaggg
//
LOCUS       DAZFRE010000409          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFRE010000409 DAZFRE010000000
VERSION     DAZFRE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.416"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>386
                     /locus_tag="ACHKUI_005432"
     CDS             <1..>386
                     /locus_tag="ACHKUI_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFO6601394.1"
                     /translation="QWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWTG
                     ELSHDRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKG
                     LDKAYSKEIYQLIKNDWKKVGSLPEG"
BASE COUNT          110 a           70 c          108 g           98 t
ORIGIN      
        1 accagtggaa gaacctggga acgactccat ttccgggaac tgctggttca tcggtgattt
       61 ttgcagaaca gcaaatttat atactgggag gggagagaaa accggggctt cgttctgtta
      121 gatcctggac gggggagctt tcgcatgatc gaattaaatg gtctgaactt ccaccagtag
      181 cttcacccga gggggtgtca ggagcatatg cctctgttat tgatggaaat atctttctgg
      241 ccggtggtgc atatttcccg ggggcggcag aaaagtatag caatggtgaa tactggtctc
      301 ataaaggact ggataaagcc tattcaaaag agatatatca gctaataaaa aatgactgga
      361 aaaaagttgg ttctttgccg gaagga
//
LOCUS       DAZFRE010000410          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFRE010000410 DAZFRE010000000
VERSION     DAZFRE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.417"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            192..>381
                     /locus_tag="ACHKUI_005433"
     CDS             192..>381
                     /locus_tag="ACHKUI_005433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601395.1"
                     /translation="MKYRTKIKRKMPYAGCSCRVFIGKLYVIATLSSVIRKRRPAILI
                     NIISLGTGIFYKQYDELNI"
BASE COUNT          119 a           70 c           74 g          118 t
ORIGIN      
        1 agttttgaga catctctatg agtctcagga cagttaaaaa ataaaaatta tttttacccg
       61 gtcactttgt aaaaaagtgg tcgggttttt gatttctgtg tttcgtgcgc ttaatcgagt
      121 ctcacgcttc agtgagcaaa ttgcccctcc ccaacagaca tcacgattca caaggtcaaa
      181 aattctgttc catgaagtac cgtacaaaga taaagagaaa aatgccttat gcgggatgta
      241 gctgtcgcgt ctttatcggg aaactatacg taattgccac tttgtcttcg gtaatcagaa
      301 aaagaagacc tgccatttta ataaatatta tttctctggg aacggggatt ttttataagc
      361 aatatgacga gttaaacatt a
//
LOCUS       DAZFRE010000411          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFRE010000411 DAZFRE010000000
VERSION     DAZFRE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.418"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..332)
                     /locus_tag="ACHKUI_005434"
     CDS             complement(<1..332)
                     /locus_tag="ACHKUI_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFO6601396.1"
                     /translation="MNKLVLVGHPGSKYQIVEHFLKEIGMNSPNYSTSNKISPEYITA
                     SLCQFYQTPEVNDVVDEREFSAVQVSTMWDSMVLELMMNNLNNKLWGWADPSIIFFLD
                     FWKNIDKS"
BASE COUNT          117 a           66 c           52 g          140 t
ORIGIN      
        1 atgcttttat ctatattttt ccaaaaatca agaaaaaata ttatagatgg atctgcccac
       61 ccccaaagtt tgttatttag attgttcatc attagttcaa gaaccatgct atcccacatg
      121 gttgagactt gaacagctga gaattctctc tcatctacta catcattaac ttctggtgtt
      181 tgataaaatt gacataatga agcggtgata tattctgggg aaattttatt acttgtagaa
      241 taatttggtg agttcatgcc aatttctttc aaaaaatgtt caactatctg atactttgag
      301 ccaggatgtc cgactagcac taatttattc atcatatttc ctctttatta tctacaagat
      361 tactttattt taaac
//
LOCUS       DAZFRE010000412          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFRE010000412 DAZFRE010000000
VERSION     DAZFRE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.419"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>364
                     /locus_tag="ACHKUI_005435"
     CDS             <1..>364
                     /locus_tag="ACHKUI_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601397.1"
                     /translation="PIVIPYDIKNLPAYNKIQGNFLSVMMLSGLFPMAFIYVLYCAFD
                     FDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKNNRMKLLRRVQIYLIIPL
                     SIALLVHLQVIPLEMVFRN"
BASE COUNT          104 a           56 c           64 g          140 t
ORIGIN      
        1 accaatagtt attccttatg atataaaaaa tcttcctgct tacaataaga ttcagggtaa
       61 ttttttatct gtaatgatgt tatctggcct cttccccatg gcctttatat acgtgttata
      121 ctgtgcattt gattttgacc agagtgttaa agataactct ggctggttat ctattttaag
      181 tataggagta ctaatagttg ttatttcagc cataataaac acgaggtatc ttgaatatga
      241 cttatcgttt aaaaataata gaatgaaact gttgagacgt gttcagatct atcttatcat
      301 tccgttgtct attgctttac tggtgcatct tcaggtaatt ccactggaga tggtttttag
      361 gaat
//
LOCUS       DAZFRE010000413          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFRE010000413 DAZFRE010000000
VERSION     DAZFRE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.420"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..258
                     /locus_tag="ACHKUI_005436"
     CDS             <1..258
                     /locus_tag="ACHKUI_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601398.1"
                     /translation="RINENAGLEKMLIGMRVKSERSTCVVSNGKLSSETKTKWEYIRS
                     TATIRIGGQTTAGLRHLFGHVRNFRLWHKELTDAQLGEVVE"
BASE COUNT          111 a           61 c          102 g           87 t
ORIGIN      
        1 cgaataaatg aaaatgccgg gctggaaaaa atgcttattg gtatgcgggt aaagtccgaa
       61 cggtccacat gtgtagtcag taacggtaag ttaagcagcg aaactaaaac caaatgggaa
      121 tatatccgga gtacagcaac cattcgcatt ggtggacaaa ctacagcagg attacgccat
      181 ttatttgggc atgtgaggaa ttttcgtctc tggcataaag agctaacaga tgcgcagctt
      241 ggggaggttg tggagtgaga gatttcacgt tgcgtttcag tgataaagca gatttcaggg
      301 catttctcag gaaacttaac tgggaagagg acgaagagct gcagaatgcc gttctggttg
      361 a
//
LOCUS       DAZFRE010000414          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFRE010000414 DAZFRE010000000
VERSION     DAZFRE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.421"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..243
                     /locus_tag="ACHKUI_005437"
     CDS             <1..243
                     /locus_tag="ACHKUI_005437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601399.1"
                     /translation="EWIKITLLRQTQKQPSFYIIIQFQLTPDERQCFSTGIKSQLLLS
                     KTHSTNNISADIILTRTPHITEFSSDSITAQIKRTG"
BASE COUNT          134 a           82 c           55 g           88 t
ORIGIN      
        1 gaatggatta aaataacact tcttcgacaa acacaaaaac agccttcatt ttacatcatc
       61 atccagtttc agttaacccc ggacgagcga cagtgtttca gtacaggaat taaaagccag
      121 cttttgctca gtaaaacaca ctcaactaat aatatctcag cagatattat cctgaccagg
      181 acacctcaca ttactgaatt ctcttccgac agtatcactg cacaaataaa aagaacggga
      241 taaatattca gaccggaaga atggtgtcac aaaagcattt aatcattacg catgaccagg
      301 aagggaaaca cttaaccaca tggtatcaac agatatcatt acgtgtgcac tcttataaa
//
LOCUS       DAZFRE010000415          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFRE010000415 DAZFRE010000000
VERSION     DAZFRE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.422"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(107..>352)
                     /gene="traF"
                     /locus_tag="ACHKUI_005438"
     CDS             complement(107..>352)
                     /gene="traF"
                     /locus_tag="ACHKUI_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6601400.1"
                     /translation="FRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMM
                     LVDPKQGSVRPLSYGFISQDDLAKQFLNVSEDFKPNF"
BASE COUNT          107 a           92 c           73 g           80 t
ORIGIN      
        1 tcctttatta tttggtcctg taaagaaata accaacaacc cccacgataa acaggctggc
       61 acctgtaaaa aaacacaaat cccctgtatg catatataac ctttggttaa aaattcggtt
      121 taaaatcttc agaaacgttc aggaactgtt ttgccaggtc gtcctgtgaa ataaagccgt
      181 atgataacgg gcgaacactg ccctgtttcg ggtcaaccag catcatggcc gggaaatatt
      241 tcacaccgag gcgctgcgcc tgcccctggt cagtccggga atccggcaac agcggattaa
      301 tcacgccatc cacggaaacg ggaataacac tcagaccata ggtatccctg aa
//
LOCUS       DAZFRE010000416          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFRE010000416 DAZFRE010000000
VERSION     DAZFRE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.423"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            20..>349
                     /locus_tag="ACHKUI_005439"
     CDS             20..>349
                     /locus_tag="ACHKUI_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImpB"
                     /protein_id="HFO6601401.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGI"
BASE COUNT           84 a           84 c           95 g           86 t
ORIGIN      
        1 ggacgcggga gttacgctga tgtttgcact ggctgatatc aacagtttct acgcctcatg
       61 tgaaaaagtt ttccgcccgg accttcgcaa cgaaccggtc atcgtactca gcaataacga
      121 tggctgtgtg atcgcgcgca gcccggaggc aaaagccctt ggcatcagaa tggggcagcc
      181 ctggtttcag gtgagacaaa tgcgcctgga gaagaaaata catgtatttt ccagcaatta
      241 tgcgctgtac cacagcatga gccaacgggt tatggctgtt ctggagtcgc tttctcccgc
      301 agttgagccc tactcaattg atgaaatgtt cattgatttg cgggggata
//
LOCUS       DAZFRE010000417          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFRE010000417 DAZFRE010000000
VERSION     DAZFRE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.424"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..196)
                     /gene="tnpB"
                     /locus_tag="ACHKUI_005440"
     CDS             complement(<1..196)
                     /gene="tnpB"
                     /locus_tag="ACHKUI_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6601402.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLC"
BASE COUNT           76 a           90 c           98 g           84 t
ORIGIN      
        1 gacacagtcc atcgccggta gaccagagga gctttacctg actgccatta cgcccacgga
       61 agataaaaac gtgacctgac atcggatcgt ctttcagcgt cgtctgcacc tttgccgcca
      121 ggccgttgaa gccgtttctc atatcggtga tgccagcgac cagccagatc tttgtccctg
      181 atggtaatgg gatcaacgtt tgagctcccg tatcagcagg ttcaggatat tttcgctgat
      241 ggcaccattc agacggagtg atccgtgccg gaacgtcact tcacagctga tattgagtga
      301 ttcaggtgtc gcagcagcta caggttgtga ccggggggag gttgtagg
//
LOCUS       DAZFRE010000418          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFRE010000418 DAZFRE010000000
VERSION     DAZFRE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.425"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>347
                     /locus_tag="ACHKUI_005441"
     CDS             <1..>347
                     /locus_tag="ACHKUI_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6601403.1"
                     /translation="DKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKV"
BASE COUNT           66 a          102 c           99 g           80 t
ORIGIN      
        1 gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc cgccagcggt
       61 ggcttcctgg caccgttcgt cgccccggat atcgtctggg acttccgcct gccgcgtgtg
      121 aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg cggctgggtt
      181 atctggcgtg atgaagaagc gctgccgcag gaactggtgt tcaacgttga ctacctcggc
      241 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
      301 tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtaca
//
LOCUS       DAZFRE010000419          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFRE010000419 DAZFRE010000000
VERSION     DAZFRE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.426"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>345)
                     /locus_tag="ACHKUI_005442"
     CDS             complement(<1..>345)
                     /locus_tag="ACHKUI_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601404.1"
                     /translation="IVGTSKAHTIIGLYSSTGGVDTSGDEKHVTISCRVKSEVDNIAV
                     RILFEHYDGEVRTSIGAANLNLTTRIISKTGQTSRVTARSVKDDATGWIFFEATLKAD
                     TTENTVGGFVQYS"
BASE COUNT           93 a           86 c           58 g          108 t
ORIGIN      
        1 agaatactgg acaaaaccac caaccgtatt ttctgttgta tctgctttta atgtagcctc
       61 aaaaaatatc cagccagttg catcatcctt aacagacctt gctgtaacac ggcttgtctg
      121 acctgtcttg ctaattatgc gggtggtaag gttcaggttt gctgctccta ttgatgtcct
      181 tacctcccca tcataatgtt caaataaaat acgaacggcg atattatcaa cttcactttt
      241 tacccgacag gatatagtta catgcttttc gtcccctgaa gtatcaaccc ctccggtact
      301 cgaatacagt ccgataatgg tatgcgcttt acttgtacca acaat
//
LOCUS       DAZFRE010000420          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFRE010000420 DAZFRE010000000
VERSION     DAZFRE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.427"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            1..344
                     /locus_tag="ACHKUI_005443"
                     /pseudo
     CDS             1..344
                     /locus_tag="ACHKUI_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           82 a           89 c          100 g           73 t
ORIGIN      
        1 tcctgcctct gaacttgctg ccgccatgaa gcagattaaa gaactccagc gtctgctcgg
       61 caaaaaaacg atggaaaatg aactccttaa agaagccgtt gaatatgggc gagcaaaaaa
      121 gtggatagcg cacgcgccct tattgcccgg ggatggggag taagcttcgt cagccgttgt
      181 ctccgggtgt cgcgtgcgca gttgcacgtc attctcagac gagccgatga ctggaaggac
      241 ggccgccgca gccgtcacac ggatgatacg gatgtgcttc gccgtataca ccatgttatc
      301 ggagagctac ccacatatgg ttatcgtcgg gtatgggcgc tgct
//
LOCUS       DAZFRE010000421          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFRE010000421 DAZFRE010000000
VERSION     DAZFRE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.428"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>342
                     /locus_tag="ACHKUI_005444"
     CDS             <1..>342
                     /locus_tag="ACHKUI_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6601405.1"
                     /translation="DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATD
                     TTVNGGLFTARGGTLAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGS
                     VEKSGSGTLTVS"
BASE COUNT           82 a           98 c          107 g           55 t
ORIGIN      
        1 gattccggtg ccgctgtcag tggtacccgg agcgacggaa aggcattcag tatcggaggc
       61 ggtcaggcgg atgccctgat gctggaaaaa ggcagttcat tcacgctgaa cgccggtgat
      121 acggccacgg ataccacggt aaatggcgga ctgttcaccg ccaggggcgg cacactggcg
      181 ggcaccacca cgctgaataa cggcgccaca cttacccttt ccgggaagac ggtgaacaac
      241 gataccctga ccatccgtga aggcgatgca ctcctgcagg gaggcgctct taccggtaac
      301 ggcagcgtgg aaaaatcagg aagtggcaca ctcactgtca gc
//
LOCUS       DAZFRE010000422          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFRE010000422 DAZFRE010000000
VERSION     DAZFRE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.429"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>341
                     /locus_tag="ACHKUI_005445"
     CDS             <1..>341
                     /locus_tag="ACHKUI_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601406.1"
                     /translation="QDLNNSQVSCKGSVDSTIADLLEKPLNNALLAIRNEHLLLMPHV
                     CSESISYLLGENGILEEIDKLYESNDHGIDNHKKGNNEINDIMINLSHILIDSLDDAK
                     VNLTPVIDSML"
BASE COUNT          119 a           55 c           64 g          103 t
ORIGIN      
        1 cgcaggactt aaataattcg caggtgagtt gtaaaggttc agttgattct acgattgcgg
       61 atttattaga aaaaccattg aataatgcct tattagcaat aaggaacgaa catctgctat
      121 taatgcctca tgtatgtagt gaatcgattt catacttact gggcgaaaat ggtatacttg
      181 aagaaataga taagctctac gaatcaaatg atcacggaat tgataatcac aaaaaaggta
      241 acaatgaaat taatgacatc atgattaacc tgtctcatat tcttattgat tccttagatg
      301 atgcaaaggt taaccttaca ccggtcatcg attcgatgct g
//
LOCUS       DAZFRE010000423          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFRE010000423 DAZFRE010000000
VERSION     DAZFRE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.430"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>340)
                     /locus_tag="ACHKUI_005446"
     CDS             complement(<1..>340)
                     /locus_tag="ACHKUI_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6601407.1"
                     /translation="IFSEDYKQSTLGLGKYFENFGAFSFDSSFSSAELDNNTVKDGQA
                     YRVMYSKIFSPTDTAFNLAGYKYQSSGYYDFNDFQYVNNGLDFYNQKSRLSVSLSQPL
                     GDELGAFYLSA"
BASE COUNT          125 a           65 c           51 g           99 t
ORIGIN      
        1 gccgacaaat aaaaagcccc taattcatct ccgagcggct gagataatga aacacttaat
       61 cttgatttct ggttataaaa atcaagccca ttattcacat attgaaaatc attaaaatca
      121 taatatccac tactttgata cttatatcct gcaagattaa atgccgtatc tgtaggagag
      181 aaaattttgg agtacataac ccgataagct tgaccatctt ttactgtatt gttatccaat
      241 tcagcagaag aaaaagatga atcaaaggag aatgcaccaa agttttcaaa atattttccc
      301 aaacctaagg ttgattgctt gtaatcctcg gagaaaatcc
//
LOCUS       DAZFRE010000424          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFRE010000424 DAZFRE010000000
VERSION     DAZFRE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.431"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>338
                     /gene="traT"
                     /locus_tag="ACHKUI_005447"
     CDS             <1..>338
                     /gene="traT"
                     /locus_tag="ACHKUI_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO6601408.1"
                     /translation="VGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVNYT
                     MITDVQIAERTKSTVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNLK
                     FEEAKPVLED"
BASE COUNT           89 a           76 c           98 g           75 t
ORIGIN      
        1 gttggtgcag cgttaggtgc cggtattacc ggatataact caaattctgc cggtgccaca
       61 ctcggtgtgg gccttgctgc cggtctggtg ggtatggctg cagatgcgat ggtggaagat
      121 gtgaactata ccatgatcac ggatgtgcag attgcagagc gtactaagtc aacggtgaca
      181 acggataatg ttgccgccct gcgtcagggc acatccggtg cgaaaattca gaccagtact
      241 gaaacaggta accagcataa ataccagacc cgtgtggtct caaatgcgaa caaggttaac
      301 ctgaaatttg aagaggcgaa gcctgttctc gaagacca
//
LOCUS       DAZFRE010000425          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFRE010000425 DAZFRE010000000
VERSION     DAZFRE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.432"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>338
                     /gene="traT"
                     /locus_tag="ACHKUI_005448"
     CDS             <1..>338
                     /gene="traT"
                     /locus_tag="ACHKUI_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO6601409.1"
                     /translation="VGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVNYT
                     MITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNLK
                     FEEAKPVLED"
BASE COUNT           91 a           74 c           97 g           76 t
ORIGIN      
        1 gttggtgcag cgttaggtgc cggtattacc ggctataact caaattctgc cggtgccaca
       61 ctcggtgtag gccttgctgc tggtctggtg ggtatggctg cagatgcgat ggtggaagat
      121 gtgaactata ccatgatcac ggatgtacag attgcagagc gtactaaggc aacggtgaca
      181 acggataatg ttgccgccct gcgtcagggc acatcaggtg cgaaaattca gaccagtact
      241 gaaacaggta accagcataa ataccagacc cgtgtggttt caaatgcgaa caaggttaac
      301 ctgaaatttg aagaggcgaa gcctgttctc gaagacca
//
LOCUS       DAZFRE010000426          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFRE010000426 DAZFRE010000000
VERSION     DAZFRE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.433"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>331)
                     /locus_tag="ACHKUI_005449"
     CDS             complement(<1..>331)
                     /locus_tag="ACHKUI_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267195202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6601410.1"
                     /translation="DFLINLIFERYIEYINHLKLSPKQKDFYFCTKFSKFHDYTKNGY
                     KYLAFDNQADAGYGLTLLLNANDDMQDSYNLLPEQELFICNAVIDNMNIYRSQFNKCL
                     RKYDLSEI"
BASE COUNT          118 a           45 c           42 g          126 t
ORIGIN      
        1 ttatttctga taaatcgtat tttcgtagac atttgttaaa ttgactccta taaatattca
       61 tattatctat tacagcatta caaataaaaa gttcttgctc agggagtaga ttataactat
      121 cttgcatatc atcgtttgca tttaataata aagtcaggcc ataccctgca tcggcttgat
      181 tatcaaatgc taaatattta tatccatttt tagtataatc atgaaatttt gaaaacttcg
      241 tacaaaaata aaaatctttt tgtttgggcg acaattttag atggtttata tattcgatat
      301 atcgttcaaa aataagatta atgagaaaat c
//
LOCUS       DAZFRE010000427          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFRE010000427 DAZFRE010000000
VERSION     DAZFRE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.434"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
BASE COUNT          119 a           63 c           29 g          113 t
ORIGIN      
        1 tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt atattataaa
       61 tataacataa tacatctata tggggtgtac atatccccac ataaaaacac atactctcat
      121 taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc attcttgtac
      181 tatggtttag tattctcaag tacttacaga gaaagaatag catattacat cctttatttt
      241 cactaatcct gacaccacta accattttaa tcattagact tatgatagat ttaacataca
      301 accaataaat tataaacaaa ataa
//
LOCUS       DAZFRE010000428          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFRE010000428 DAZFRE010000000
VERSION     DAZFRE010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.435"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..301
                     /locus_tag="ACHKUI_005450"
     CDS             <1..301
                     /locus_tag="ACHKUI_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6601411.1"
                     /translation="LSDDELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVT
                     VPAVMLEKVMALAGSEMKRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
BASE COUNT           80 a           65 c          111 g           64 t
ORIGIN      
        1 cctgtcagac gatgagctgg aaaccgtcat gcagcggctg gacgatgctt ttgtatatgg
       61 cgcatgtgac agagttgttt ccgatattgt caatgaactg atggaagaaa agcgcgccag
      121 ccgtcatgtg acagtcccgg cggtgatgct ggaaaaagtg atggcgctgg caggtagtga
      181 aatgaagcgc ctgtatgccg tcgggagtga gaacgggggc gacggtgacg cattcgtcag
      241 ggaggaactc gaagcaatgg acgttgtgtt gcaggcgctg gacggggaga cgatgtcatg
      301 aatatcagca cagaaacccg
//
LOCUS       DAZFRE010000429          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFRE010000429 DAZFRE010000000
VERSION     DAZFRE010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.436"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>317
                     /locus_tag="ACHKUI_005451"
     CDS             <1..>317
                     /locus_tag="ACHKUI_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6601412.1"
                     /translation="HGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQP
                     THRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKP
                     VGW"
BASE COUNT           71 a           82 c          100 g           64 t
ORIGIN      
        1 tacatggcat cagccacggt tcggccggag caagaagcat cgccacaatg gcaacccgga
       61 gaggctacca gatgggacgc tggcttgctg gcaggctcat gaaagagctg gggctggtca
      121 gctgtcagca gccgactcac cggtataaac gtggtggtca tgaacatgtt gctatcccta
      181 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
      241 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      301 gaaaaccagt gggctgg
//
LOCUS       DAZFRE010000430          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFRE010000430 DAZFRE010000000
VERSION     DAZFRE010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.437"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>313
                     /locus_tag="ACHKUI_005452"
     CDS             <1..>313
                     /locus_tag="ACHKUI_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6601413.1"
                     /translation="PLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDG
                     WAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRY
                     VL"
BASE COUNT           77 a           79 c           97 g           60 t
ORIGIN      
        1 accgctgctg aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc tgtcgcgaca
       61 ctcagaactg gcgaaagcgt tcgcatacgc cctgaaccag tggccggcgc tgacgtacta
      121 tgcagatgat ggctgggctg aggcggacaa taacatcgct gaaaatgcgt tgcggatggt
      181 cagtctgggc cgcaaaaact acctgttctt cggttcggat catggaggag agcggggagc
      241 gctgctgtac agcctgatcg ggacgtgcaa actgaacgga gtggagccag aaagctacct
      301 ccgctatgtc ctt
//
LOCUS       DAZFRE010000431          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFRE010000431 DAZFRE010000000
VERSION     DAZFRE010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.438"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>303
                     /locus_tag="ACHKUI_005453"
     CDS             <1..>303
                     /locus_tag="ACHKUI_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6601414.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLA"
BASE COUNT           88 a           77 c           77 g           61 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggc
//
LOCUS       DAZFRE010000432          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFRE010000432 DAZFRE010000000
VERSION     DAZFRE010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.439"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>302
                     /locus_tag="ACHKUI_005454"
     CDS             <1..>302
                     /locus_tag="ACHKUI_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO6601415.1"
                     /translation="TEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKVQLREYGRS
                     VNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQSRHTYAC"
BASE COUNT           97 a           67 c           70 g           68 t
ORIGIN      
        1 actgaagcaa gtacaaacag ggttgtgcat cttatccagc ccgccatcag tgtcctgaaa
       61 aatcaggctg aaatgacaag actgggtaag cagcaccaca tcaaggttca actacgtgaa
      121 tatggacgtt cagtgaatca tgaatgtact ttcgtattta acccccaggt agttagaaaa
      181 agcaaacagg taggttttat ctacaaggta gattccattg gcgactcatg ggaaacagcc
      241 attaagcgtg caggcatcag gcatcgaaag gcataccagt cacgacacac ttatgcgtgc
      301 tg
//
LOCUS       DAZFRE010000433          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFRE010000433 DAZFRE010000000
VERSION     DAZFRE010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.440"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>300
                     /locus_tag="ACHKUI_005455"
     CDS             <1..>300
                     /locus_tag="ACHKUI_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6601416.1"
                     /translation="NSETATKASEKNAKSSQTAAKTSETNAKDSEANAKVSETAAANS
                     AKASAASQTAAKASEDAAREYANQTAEPYRYVLQPLPDVWIPFNDSLDMITGYSPG"
BASE COUNT          104 a           73 c           84 g           39 t
ORIGIN      
        1 aaatagcgag acagccacaa aggccagcga aaaaaacgca aaatccagcc agacggcagc
       61 gaaaaccagt gagacgaatg ccaaagacag tgaagccaac gcaaaggtga gcgaaacagc
      121 ggcggcgaac tcggcgaaag catcggcagc aagccagacg gcagcaaaag caagtgaaga
      181 tgctgccaga gaatacgcaa accagacagc agagccgtac agatatgttt tacagccgct
      241 gccggatgtg tggataccct ttaatgattc gctggatatg attacgggct attctccggg
//
LOCUS       DAZFRE010000434          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFRE010000434 DAZFRE010000000
VERSION     DAZFRE010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.441"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>294
                     /locus_tag="ACHKUI_005456"
     CDS             <1..>294
                     /locus_tag="ACHKUI_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6601417.1"
                     /translation="SHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQ
                     ALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEH"
BASE COUNT           85 a           69 c           57 g           83 t
ORIGIN      
        1 agccatgatg tttttgatcc ttatcccgcg gcgttacatg gtcaatttga ttccatttcc
       61 atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc
      121 aatgcggcgc aggccttaac tgacgatgga actctatacg gagccacaat tcttggtgac
      181 ggcgttgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc
      241 ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga gcat
//
LOCUS       DAZFRE010000435          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFRE010000435 DAZFRE010000000
VERSION     DAZFRE010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.442"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>291
                     /locus_tag="ACHKUI_005457"
     CDS             <1..>291
                     /locus_tag="ACHKUI_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601418.1"
                     /translation="QWQSLPVLPADGTQVSFPYAGEWLAEDEIRAVLGAVRDAVCSVS
                     YQVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVV"
BASE COUNT           54 a           82 c          100 g           55 t
ORIGIN      
        1 gtcagtggca gtcgctgccg gtacttccgg cagacggaac gcaggtgtct ttcccgtatg
       61 ccggtgagtg gctggcagag gacgaaatca gggccgttct tggtgcggta cgcgatgcag
      121 tatgcagtgt cagttaccag gtggcagaag atgcgcggcg tatccgtgcg gcgctgacca
      181 ccaccggtca gacgttgctg acccgccaga cgcgccgctt tcgcctggtg gtgaaggaaa
      241 gcgatcaccc ctgctggctc gatgaagacg acgaaaacct gcccgtggtg c
//
LOCUS       DAZFRE010000436          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFRE010000436 DAZFRE010000000
VERSION     DAZFRE010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.443"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            complement(<1..>284)
                     /locus_tag="ACHKUI_005458"
     CDS             complement(<1..>284)
                     /locus_tag="ACHKUI_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6601419.1"
                     /translation="ELLSRIDGYKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDN
                     AMWTIPAEREPLPGVKFSYRGSKMRTPHLVPLSQQAVAILAELQTWAGENG"
BASE COUNT           72 a           68 c           71 g           73 t
ORIGIN      
        1 ccattttcac ctgcccatgt ctgaagttct gccagtatgg ctacagcctg ttggctgagt
       61 ggcacaagat gtggtgttcg catcttggag ccccggtatg aaaattttac gcctggcaga
      121 ggttcgcgtt cggctggaat agtccacatg gcattgtcaa tatcaatttc agaccagcgg
      181 gcatatctga gttcactgga acgaataaaa accagtaaat tcagcataac tgccagtcgg
      241 gtgacaggct ggcctttata accgtcaata cgcgacagaa gttc
//
LOCUS       DAZFRE010000437          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFRE010000437 DAZFRE010000000
VERSION     DAZFRE010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.444"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>282
                     /locus_tag="ACHKUI_005459"
     CDS             <1..>282
                     /locus_tag="ACHKUI_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO6601420.1"
                     /translation="AWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGYSGKRAS
                     ESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVML"
BASE COUNT           65 a           78 c           93 g           46 t
ORIGIN      
        1 agcctggaca ccgggccggg aagacgtgct cctctcactt gccggagaga ttgaggatga
       61 agacagcacg ctcgctgaac gtcaggaagc acgggcggag cggtttaccg gatacagcgg
      121 aaagcgggcc agtgaatccg cacaggcact tgatgaagtg gaaagactgg ccgcgatgat
      181 cccgcccggt cagcccattc ttgtggggca tcacagcgaa cgccgcgccc gtcgtgatgc
      241 gcagcgtatt gaaaacggca tgaaacgtgc cgtgatgctc tt
//
LOCUS       DAZFRE010000438          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFRE010000438 DAZFRE010000000
VERSION     DAZFRE010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.445"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>281
                     /locus_tag="ACHKUI_005460"
     CDS             <1..>281
                     /locus_tag="ACHKUI_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFO6601421.1"
                     /translation="NTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLMVLDHIN
                     LIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIA"
BASE COUNT           56 a           71 c           87 g           67 t
ORIGIN      
        1 aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca gtcgtttctg
       61 gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cgctggtgct gatggtactg
      121 gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga
      181 gcctttccgc tgtttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg
      241 caaccggcca ttaaccggct gtggggatgg ggaattattg c
//
LOCUS       DAZFRE010000439          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFRE010000439 DAZFRE010000000
VERSION     DAZFRE010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.446"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..>281
                     /locus_tag="ACHKUI_005461"
     CDS             <1..>281
                     /locus_tag="ACHKUI_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="similar to VirB4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6601422.1"
                     /translation="TLRFNPFANITDIDQSAERVRDQLSVMASPNGNLDEVHEGLLLQ
                     AVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLD"
BASE COUNT           64 a           74 c           84 g           59 t
ORIGIN      
        1 accctgcgtt ttaacccgtt tgcgaacatc accgatattg accagtcagc ggagcgtgtc
       61 cgtgaccagt tgtcagtgat ggccagcccc aacggtaacc tggatgaagt gcatgaaggt
      121 ctgctgctgc aggcggtcag ggcctcctgg ctggccaaag agaacagggc acgtattgat
      181 gacgtggtgg atttcctgaa aaacgccagt gacagcgagc agtatgccgg gtcaccgacc
      241 atccgcagcc gtctggatga aatgattgtg ctgcttgacc a
//
LOCUS       DAZFRE010000440          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFRE010000440 DAZFRE010000000
VERSION     DAZFRE010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.denovo.447"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..245
                     /locus_tag="ACHKUI_005462"
     CDS             <1..245
                     /locus_tag="ACHKUI_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6601423.1"
                     /translation="DPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNG
                     VLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
BASE COUNT           59 a           73 c           87 g           57 t
ORIGIN      
        1 atgatccggc gcaggtggcc tggatgaaac gccagacgcc gcccacactg gagagcaaaa
       61 ttatcctcgt gcagggcagt atcccggaga tgcagaagtc tctggacagc cgtgtttact
      121 ttgaccagaa cggtgtgctc tgccagcgcc tcggcattga tcaggtcccg gcacgggtga
      181 gcgccgttcc cggcgatcgc ttcctgaagg tggaatttat tccggcagag gagggcagaa
      241 aatgaagcga agcctgtggc tgctgatgtt attcct
//
LOCUS       DAZFRE010000441        10655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.140, whole genome shotgun sequence.
ACCESSION   DAZFRE010000441 DAZFRE010000000
VERSION     DAZFRE010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.140"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            28..501
                     /gene="dfrA1"
                     /locus_tag="ACHKUI_005463"
     CDS             28..501
                     /gene="dfrA1"
                     /locus_tag="ACHKUI_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO6601424.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            596..1120
                     /gene="sat2"
                     /locus_tag="ACHKUI_005464"
     CDS             596..1120
                     /gene="sat2"
                     /locus_tag="ACHKUI_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFO6601425.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1178..2013
                     /gene="aadA1"
                     /locus_tag="ACHKUI_005465"
                     /pseudo
     CDS             1178..2013
                     /gene="aadA1"
                     /locus_tag="ACHKUI_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            2042..2539
                     /locus_tag="ACHKUI_005466"
     CDS             2042..2539
                     /locus_tag="ACHKUI_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601426.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2600..2971
                     /locus_tag="ACHKUI_005467"
     CDS             2600..2971
                     /locus_tag="ACHKUI_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601427.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3381..3758
                     /locus_tag="ACHKUI_005468"
     CDS             3381..3758
                     /locus_tag="ACHKUI_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601428.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3808..3933
                     /locus_tag="ACHKUI_005469"
     CDS             3808..3933
                     /locus_tag="ACHKUI_005469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6601429.1"
                     /translation="MKVVYLAIQAATKKWTMPIRNWKPAMNRFMIEFGDRLNGHL"
     gene            complement(3989..5605)
                     /locus_tag="ACHKUI_005470"
     CDS             complement(3989..5605)
                     /locus_tag="ACHKUI_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFO6601430.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(5606..7132)
                     /locus_tag="ACHKUI_005471"
     CDS             complement(5606..7132)
                     /locus_tag="ACHKUI_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFO6601431.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(7135..8802)
                     /locus_tag="ACHKUI_005472"
     CDS             complement(7135..8802)
                     /locus_tag="ACHKUI_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6601432.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8799..>10655)
                     /locus_tag="ACHKUI_005473"
     CDS             complement(8799..>10655)
                     /locus_tag="ACHKUI_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6601433.1"
                     /translation="KILPIINSKDRFDPKVRSELVEHVVQEHKVTKATVYKLLRRYWQ
                     RGQTPNALIPDYKNSGAPGERRSATGTAKIGRAREYGKGEGTKVTPEIERLFRLTIEK
                     HLLNQKGTKTTVAYRRFVDLFAQYFPRIPQEDYPTLRQFRYFYDREYPKAQRLKSRVK
                     AGVYKKDVRPLSSTATSQALGPGSRYEIDATIADIYLVDHHDRQKIIGRPTLYIVIDV
                     FSRMITGFYIGFENPSYVVAMQAFVNACSDKTAICAQHDIEISSSDWPCVGLPDVLLA
                     DRGELMSHQVEALVSSFNVRVESAPPRRGDAKGIVESTFRTLQAEFKSFAPGIVEGSR
                     IKSHGETDYRLDASLSVFEFTQIILRTILFRNNHLVMDKYDRDADFPTDLPSIPVQLW
                     QWGMQHRTGSLRAVEQEQLRVALLPRRKVSISSFGVNLWGLYYSGSEILREGWLQRST
                     DIARPQHLEAAYDPVLVDTIYLFPQVGSRVFWRCNLTERSRQFKGLSFWEVWDIQAQE
                     KHNKANAKQDELTKRRELEAFIQQTIQKANKLTPSTTEPKSTRIKQIKTNKKEAVTSE
                     RKKRAEHLKPSSSGDEAKVIPFNAVEADDQEDYSLPTYVPELFQDPPEKDES"
BASE COUNT         2896 a         2600 c         2262 g         2897 t
ORIGIN      
        1 taaaatgtta acctctgagg aagaattgtg aaactatcac taatggtagc tatatcgaag
       61 aatggagtta tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga acagctcctg
      121 tttaaagcta ttacctataa ccaatggctg ttggttggac gcaagacttt tgaatcaatg
      181 ggagcattac ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat
      241 gagaacgtat tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg
      301 gatcatgtca ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat
      361 acactacata tatctacaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc
      421 cccagcaatt ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac
      481 caaatctggc aaaagggtta acaagtggca gcaacggatt cgcaaacctg tcacgccttt
      541 tgtaccaaaa gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc gtcatatgaa
      601 gatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc atttcattgt
      661 tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta ccagaagtgt
      721 gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact ctgcttgcta
      781 tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa catggaacga
      841 tctagcctct atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca
      901 cagtctcatc gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt
      961 agagacacaa acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct
     1021 cggcggcatt gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat
     1081 gtactggtac tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac
     1141 cgcttcgcgg cgcggcttaa ttcaggagtt aaacatcatg agggaagcgg tgatcgccga
     1201 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1261 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1321 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1381 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1441 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1501 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1561 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1621 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1681 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     1741 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1801 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1861 gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     1921 tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     1981 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagagagc tggggaagac
     2041 tatgcgcgat ctgttgaagg tggttctaag cctcgtactt gcgatggcat cggggcaggc
     2101 acttgctgac ctgccaattg ttttagtgga tgaagctcgt cttccctatg actactcccc
     2161 atccaactac gacatttctc caagcaacta cgacaactcc ataagcaatt acgacaatag
     2221 tccatcaaat tacgacaact ctgagagcaa ctacgataat agttcatcca attacgacaa
     2281 tagtcgcaac ggaaatcgta ggcttatata tagcgcaaat gggtctcgca ctttcgccgg
     2341 ctactacgtc attgccaaca atgggacaac gaacttcttt tccacatctg gcaaaaggat
     2401 gttctacacc ccaaaagggg ggcgcggcgt ctatggcggc aaagatggga gcttctgcgg
     2461 ggcattggtc gtcataaatg gccaattttc gcttgccctg acagataacg gcctgaagat
     2521 catgtatcta agcaactagc ctgctctcta ataaaatgtt aggcctcaac atctagtcgc
     2581 aagctgaggg gaaccactag tgtcatacga acctccaaga gacggttaca caaacgggta
     2641 cattgttgat gtcatgtatg acaatcgccc aagtaagtat ccagctgtgt tcagaacgta
     2701 cgtccgaatt ccagactatg tcgaactaga taacgcggag gagctggccg acgcaatcat
     2761 tcgagaggtt gagttaaagg aggcatggct cacaagggtc ttggggaact cagccgatcc
     2821 tgacgcttct gtgggcaagt gggaccggcg cctagcgcag tgcggtggta ttgatgagat
     2881 gtcaagtggg gtgcaggcaa catacttcag tccaaacggt cagctggtcg gcttagtggc
     2941 atacattgat gccgaatgga tggatgaata gctctgacgt tacgattcag gtgagacaac
     3001 cagatgggca ttgcgtacaa gctcagttgc caccgatgtg gctacgagtc tgatcttctt
     3061 tacctaggcc aaggtatggc gatgcttcct gagcaaatag tcggaacgtg cacatgctgc
     3121 gacaatctaa cgacgatttc tataaatgaa agctacggag tatgctcact ttgtggggct
     3181 aaggatcgtg tgatatttac aagctatcag catgaggcaa ggccaagaaa tccctgctat
     3241 ctagagagaa gtttcaaata tcaatgccca agatgccatg ccttcagtat ggagcctccg
     3301 gatctaccgg agatgctttg ggactaatgc tccgctttgc ctcgggtatc cgttcgacct
     3361 atgtttaaga ggaaacgcac gtgtctgccg ccaaagctct tgcctatgca ctttgtgaca
     3421 agttggaaag caaaaaagtt gatgaagtcg tacgaaatct tgtttctagc ggctggaata
     3481 ttagggaagt ggcctgggaa gcttcgtcct ttgatcatga aatgccctac tttgacacgg
     3541 ggcgggactt cgatattgga tttcctgaca cgctgtcatc caagattgag tttttgaccg
     3601 cgaatggatc aatcgtccaa gcacgtgctc gcctgcactt caagcaggct ctaatttttg
     3661 ccaaggcgtc caaacatgtc gccgcgctca agaactcatt ggattactac tatggatcgt
     3721 ctgtaatccc ccctcaaaac cacggaggct atttgtagaa aattccatta atatgaactc
     3781 aaggtgttcc ctacggacga atcagcgatg aaggtggtgt acctggctat ccaggcggcg
     3841 accaagaaat ggacgatgcc gatccgcaac tggaagccgg ccatgaaccg ttttatgatc
     3901 gaatttggtg accgtttaaa tggccacctt taactgaccg gcaaaagcac ttacacaaaa
     3961 gggtttacag gctctttcag tcattccttt aatgcgtaaa ttgctctcta accactctct
     4021 cagcccaccc ttccatatcc tctctcgata cttgcagctc ccccaacccc tttggatgat
     4081 tcacccctct atgcccgtct tgtccatata attgatcaaa caaactattt ggccaattca
     4141 atgagctctt caccactcca cgccgtatct tttcaaaatc attccgccat gtttcgctat
     4201 caacgccact caacactttg gtagaaagca ttttcacccc atccgatgca tccacctcta
     4261 gcaatacaaa ttcactgcca ttacgttgca ccccaacggc tttaatcact cttggtgagc
     4321 catctttaca cagatgtaat cggctacgcc caacttttgg caaaaccaag gtttcttcaa
     4381 aaagcacccg acatgcaaat ttagttttta gaattgaaag tagctcatca aaagcagcaa
     4441 atcgattagc aaaaatagag ttgtaattgg ttgcatcctg cttcccaccc acatctgccg
     4501 ctgctaggac accacctaag tgtggctcat cagtactaac caaattacta ttttcacttg
     4561 ttgttgcttc ttctttttct aaaatagttt ggctactttt tcttgccctg cgagattttt
     4621 caattcgact aggtctacta aaacttatcc atgtcggctc agcctctatg actcgtgtgt
     4681 ctgtgtctat attggctgtt tcctcatcgt ccaactgtag atgctcatca tcaacaactg
     4741 actctgttga aaccgctatg tgctgcgtac taccatcacc tgcctccttt tcctgaaaag
     4801 aggcatggct aatagctgtt gtgctaggaa gcatagcgtt gatttctaaa cctactattt
     4861 cctcaactaa ataatcctta tcctcgttag aagaacgtcc ttttacatgt aatttccaac
     4921 cctgcatggg cggagggtca aagctaaagc accagctttc aacgccgttc ttaatttctc
     4981 tattttgttg atagtgccta aaaatacttt catacgaatc cataacatct tgatccgaaa
     5041 acaaccaaac caaaagctgc actacggccg actgctctaa cgccccttta ggaaacgatg
     5101 aactgggtaa gatccttatc tctaaatgat ctcgctcaac ctcatactga acgtcgaact
     5161 cttgctgtaa agcggtactg ctcaaacagc ttcgacagaa ataggagtta attaaaaata
     5221 atgaccgagc tagctctaac tgtgggatgt gataacgagt cttgctatat ggtgtttcag
     5281 cttcgtaaat aaatgaactc tgcgctccat ccttgttgcg tacggaagca aaggacgaga
     5341 gattaggaaa atcgattatt ttggctcgtt gaagatccga tgcttcaaac tcaatcacac
     5401 gatccgcctt atttatttgc tttgttgtac tgttaaccac cttagctcga cttaataaag
     5461 gcaaatgagt aaatcgagtc catttccgtt cttgcattgg attaaaccaa acaaaaatac
     5521 gccactcctt atgacccgag ttacggaata aatgaccaat atgtacaacc tgcacattgt
     5581 cattaaatgt agctagccta accactcact cttccccata aaccatttcc aagaatcttt
     5641 gtgcttcctc agttatccgc tctttagata acgttgcact tcttaataat cgccagcgcc
     5701 taagctcaac atcttcctgt ttaagcttaa taaaagcttg gctaatccgt ctaatttgat
     5761 aatcttccac actctctgag taacgcttta agcaaagcgc taccaaaggc agtttctgta
     5821 gattttttgc aagagaggtt ccgttaggag tttgctttaa cagccagctc gatgtcgctc
     5881 ttgggtgatc aaggctacta tctagacgct ttatgattct taatagttgt cgtacagcaa
     5941 ttcgatctct ttggttccaa tcaactctag gggcgggtgc cagacgctct tgttgatgct
     6001 gttgattcca gtgaactagc caatccctgt catgtcggta aagccaagcg tatagcaccc
     6061 caccctctaa agactgtctt gccgccttaa ttccttggta tttatgcact agttgctgcc
     6121 agtctttatg cttaacagat aaatcttcag agttaggttg cacagactgg ctaacaggtc
     6181 ttgtcgttat agagtgctca gtaagagcac ttgcctgctg tagcgcttct ataaccgtta
     6241 gttttggcaa taaggcttgc cacacaatac tatgctgtaa ataactaaag gcttttctat
     6301 gcttacggaa tatacttttc agccaacacg tgtccttgtt ctctgccaac tttaaatcca
     6361 gtttctctag tgcctcatca ctaaaagtct gcctcactct ctccgccacc aagtcatgac
     6421 gaatgtgctt gcttttggtt agccctagat cctgcgctaa gcgctgataa aacagcgtcc
     6481 actgctcaag gcttggggaa agctcttgcg ctcgtggagc atctaacaga ggggctatat
     6541 aagcagctag tgctgttaat tgagataggg agtctttggg gtagtctgaa agcagctcag
     6601 tatgacccaa agcccaaaat tgatgtcggt gatcatctac agctctatca aagaagacta
     6661 aagcaccgtg ttttggacaa tatggcaaag cgggcaaata ccaatctcgt tgccaaaagg
     6721 cttccccata cctatttagc tgaagagcaa cgcaatcagg gcagtatcta aagcggttat
     6781 cgctcttaac tctagaagca gcgactccta gcattaaatg caccgcacct tgcgcttggt
     6841 actccattaa ccgaatagct tcgtctcggc gctccttgcc tacaaacgga gcatataaag
     6901 ggaataaggt atgctcataa ataagctgct gaacagcgta acgtcctgtt tgatgtagat
     6961 gtcttgctat cacacctaaa tgcgagggca gacctaaggt agcgaccacc ttgcggttgc
     7021 catacacctc atccaacagc tgcttaggac taacaatccc ttgataaacg cctgcccgtg
     7081 caatagtgct ataaatcagc tcattcgagt acggaacagg aaagtttctc atggctaacc
     7141 tgcttgttta aataagctcg ccatatccac tattacccct ttccctttta accgttcatg
     7201 catggttttt tcaggttggc gttgtgaata gatataacgt aaatccgtat caggcaagct
     7261 atcccagtcg ctgggcttga ctacctttat agccgaaacc tttttactct tggagggctt
     7321 ttctagttct gacactaccg tttcgccttc catcaaccac tgcaaaacaa gaggcagtaa
     7381 cttttgtctt gtcatcgttg gattctggct aaacgctttt ttaatagtgg gaattaacag
     7441 gcttgaatcg taatcctctt tcagcatcag atataaatga cgctgatctt cggtatctaa
     7501 ctcttgaagg gctttttctt ctggtgtttg ttcttgtatc gctgcgatat ctagctgaag
     7561 ttggattaac cgtttatcaa tctcgggaac gactagatca gaataacgag caatgcgttc
     7621 tgggataccc gagcgtaatg cctctagcat ggggtgcaca ggctttaact catcttgata
     7681 cacttgccgc aataaaccag cggtaatacg ctcattgcct aaagctagcg cacggagctg
     7741 agcgagtaca aaaagtttta ctacaatgtc catcactcct tggcttagct cataccacac
     7801 atcacggacc tcatccgata acagcgcatc tttgcgttgt aaaagctgta attgccaaag
     7861 attatccgta aaagcgatcc actcttgatt gggctttcca cgttgcgttt gttgtatagg
     7921 atcccagaat atagctccaa accctgcccc tctacgtgca gaccgcaaat cagcctcaaa
     7981 aatctctcgt gctttagggg taccaatcaa catcactggt acgccaataa tattcaccat
     8041 cgtcacaaaa aagttcagca tctcttgaga tccacccgaa cgagagcggc ttaaatgctg
     8101 aatttcatca ataaccaaca accctaaagc atgtgcattg gctatttgcg acatcaaagc
     8161 caacatggtt tctataccat gacgttttaa gccataacga cgctcatagt tcgagcccaa
     8221 ggctcgatcc aacgctctga aaaaattcaa gcagatttct tttagcgaac cattatgcga
     8281 gcagtctatt ttcaaataca ccacctgctc tacattgagt tcacgatggt aaatcacctg
     8341 aggatacgtg gctagaatac gatgaagaga ggtcgtcttc ccactaccag aacaaccaat
     8401 taacaataag ctttgtgccg tagatcgtgc ctcctcaaag cgaaatgtct ccaactctcc
     8461 cgtttgaaca cgctcataac cattttgtaa atgcttttgt aaatctcctg ttttaggatt
     8521 tctgcctacg tagccacctc gaatcatgac cgaaatacgc tcacttagta gcaaatgcgt
     8581 acctaatggc tgaaaatagt catctggaat acgacaaatg gtatgagctc tgataacacg
     8641 ggacttttgc aagtcagagg aagtaagctg taaagaggat ttcagtgatg cagcactatt
     8701 cactgactct tgtaatggtg gtaaggcctc gataaaagga ttatcacgat aagcctctac
     8761 ccccgtatca cgataaactg cttgaatccg ggtagcactc atgactcatc cttttctggt
     8821 ggatcctgaa ataattcagg cacgtatgtg ggtaggctgt aatcttcttg atcatccgct
     8881 tccactgcgt tgaaaggaat aactttagcc tcatcacctg aagagcttgg cttcaaatgc
     8941 tccgcacgtt ttttacgctc cgaggtcacg gcttcttttt tattagtttt aatctgctta
     9001 atgcgtgttg atttgggctc agtagtactg ggcgttaact tattcgcttt ctgaatggtt
     9061 tgctgaataa acgcctcaag ctccctgcgt ttagttaact catcctgctt cgcattggct
     9121 ttattgtgtt tttcttgtgc ttgtatatcc caaacctccc aaaatgagag acctttaaac
     9181 tgccgactac gttccgtcag attacagcgc caaaatacac ggctgccaac ttgcggaaac
     9241 aaataaatcg tatcaaccag cactgggtca taagccgctt ctaaatgttg aggtctagct
     9301 atatcagtgc tccgctgcaa ccaaccctca cgcagaatct ctgaccccga gtaatacaaa
     9361 ccccacaaat taacgccaaa tgaagaaata gagacctttc ggcgaggcag taacgctact
     9421 cgcaactgct cttgctccac agcccttaaa ctacctgtac gatgctgcat accccattgc
     9481 catagctgga caggaataga cggtaaatct gtaggaaaat cagcatctcg atcgtattta
     9541 tccatcacca gatggttatt tctgaataag atcgtacgca aaataatttg tgtgaactca
     9601 aataccgaca gagatgcatc taacctatag tctgtttcac catggctttt gatccgactg
     9661 ccctctacaa tgccaggtgc aaaggactta aactcggctt gtagtgttct aaaagtgctt
     9721 tccactatgc ctttagcatc gccacgtcta ggtggagcac tttccactcg cacattaaaa
     9781 ctagaaacta aggcttcgac ctgatgactc attaattcgc cacggtccgc tagcaacaca
     9841 tctggcaaac ctacacacgg ccagtctgag ctactaatct caatatcatg ctgggcacaa
     9901 atggccgttt tgtcagagca agcatttaca aaagcctgca tcgccaccac ataagacgga
     9961 ttttcaaagc cgatataaaa gcccgtgatc atccgactaa acacatcaat cacaatgtaa
    10021 agcgttggtc ttcctatgat tttttggcga tcatgatgat ccactaaata aatatcagca
    10081 atcgtggcat caatctcata acgactccca gggcctaacg cctgagaagt ggctgtacta
    10141 cttaagggtc gtacgtcttt tttatatacc cctgctttaa ctctagactt taagcgctga
    10201 gctttagggt attctcgatc ataaaaataa cgaaactgac gtagtgttgg gtaatcctct
    10261 tggggaatgc gaggaaaata ctgagcaaac aagtccacaa atcgtctata ggcaacggtg
    10321 gtctttgtac ctttttgatt taacaggtgc ttttctatgg tcaacctaaa aaggcgttca
    10381 atctcgggcg ttaccttggt tccttcaccc ttaccatatt ctctggctcg gccaatcttt
    10441 gctgttcctg tcgctgaacg tctttcccct ggtgcaccgc tgtttttgta gtcaggaatt
    10501 aatgcattag gcgtttgacc acgctgccag taacggcgta acaacttata aactgtagcc
    10561 ttagtaacct tatgttcttg gaccacatgc tcaacgagtt cgcttctgac tttagggtcg
    10621 aaacgatcct tactattaat aataggaaga atttt
//
LOCUS       DAZFRE010000442         6810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.177, whole genome shotgun sequence.
ACCESSION   DAZFRE010000442 DAZFRE010000000
VERSION     DAZFRE010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.177"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            18..545
                     /locus_tag="ACHKUI_005474"
                     /pseudo
     CDS             18..545
                     /locus_tag="ACHKUI_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            566..1039
                     /gene="dfrA14"
                     /locus_tag="ACHKUI_005475"
     CDS             566..1039
                     /gene="dfrA14"
                     /locus_tag="ACHKUI_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFO6601434.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            1114..1389
                     /locus_tag="ACHKUI_005476"
                     /pseudo
     CDS             1114..1389
                     /locus_tag="ACHKUI_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            1389..2225
                     /locus_tag="ACHKUI_005477"
     CDS             1389..2225
                     /locus_tag="ACHKUI_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO6601435.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2332..2736)
                     /locus_tag="ACHKUI_005478"
                     /pseudo
     CDS             complement(2332..2736)
                     /locus_tag="ACHKUI_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3086..3517)
                     /locus_tag="ACHKUI_005479"
     CDS             complement(3086..3517)
                     /locus_tag="ACHKUI_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO6601436.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(3585..6296)
                     /locus_tag="ACHKUI_005480"
     CDS             complement(3585..6296)
                     /locus_tag="ACHKUI_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO6601437.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAELQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(6308..6640)
                     /gene="mobA"
                     /locus_tag="ACHKUI_005481"
     CDS             complement(6308..6640)
                     /gene="mobA"
                     /locus_tag="ACHKUI_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO6601438.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
BASE COUNT         1591 a         1905 c         1601 g         1713 t
ORIGIN      
        1 aactgaagga acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact
       61 ggttgcctgt cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg
      121 ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc
      181 tcatttggct caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac
      241 aggagggtgc atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg
      301 gagcggattt gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc
      361 tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg
      421 atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc
      481 cgcagctcga tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc
      541 gcaccgttaa cccaggatga gaaccttgaa agtatcattg atagctgcga aagcgaaaaa
      601 cggcgtgatt ggttgcggtc cagacatacc ctggtccgcg aaaggggagc agctactttt
      661 taaagcattg acctacaatc agtggcttct ggtgggtcgc aagacgtttg aatctatggg
      721 cgcactcccc aataggaaat acgcggtcgt tacccgctca ggttggacat caaatgatga
      781 caatgtagtt gtatttcagt caatcgaaga ggccatggac aggctagctg aattcaccgg
      841 tcacgttata gtgtctggtg gcggagaaat ttaccgagaa acattaccca tggcctctac
      901 gctccactta tcgacgatcg acatcgagcc agagggggat gttttcttcc cgagtattcc
      961 aaataccttc gaagttgttt ttgagcaaca ctttacttca aacattaact attgctatca
     1021 aatttggaaa aagggttaac aaagctatgc aatcgacggc aaaaagcttc gttcgcttcg
     1081 cgcactacgc ctttttccgc gattgatagc gacgatatgg ttgtttgcca tggtgatccc
     1141 tgcatgccga acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt
     1201 gggcggctcg gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa
     1261 gagaactggg cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg
     1321 gggatcgaag cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact
     1381 tggggttgat gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc
     1441 tcattgcgga caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg
     1501 caatcgtcaa gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact
     1561 atctggtatg gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga
     1621 tgttgctcga atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact
     1681 accaggcgac cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac
     1741 ccctgccttc tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc
     1801 gcgatgatca aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc
     1861 aaatgatgag caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca
     1921 tcatgttctc cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg
     1981 gctttggcgc cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc
     2041 ctagacgcat tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc
     2101 gcctgctcga ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag
     2161 aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat
     2221 actagatatc aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat
     2281 cgctggccaa agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa
     2341 acgcagctga aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc
     2401 atcaaccagc aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc
     2461 gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg
     2521 tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata
     2581 gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc
     2641 aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg
     2701 atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg
     2761 tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg
     2821 ttgctagaca ttgccagcca gtgcccgtca ctcctgccag acatgctgat taattttgcc
     2881 cttattaaca tcacagacag aaagaacgag ggtaccaata ccattgatgg taactggcag
     2941 gccgatgaaa gacgccgtta ccgggataat gtaagaatct atttttgagt atgtcaacat
     3001 aacctgaggc aaagtacctc aggttccagt gtagtaatca gccaacctga cagtcttctg
     3061 ttagccctac agatatgtcg tggagctaac cgcgcatgtc aaagccggat ttttgaaaat
     3121 ctctataaac ttccggattg ttgaaggccg ggaatttagc tttatgggct gctgacttta
     3181 tcgcttcgca ataggcttta tcgccattac tggttgatat ttttaacgcc gtgccatccc
     3241 gggagaattc catatgcaac ctgcattttt tccttttcca tttttgcgga tcatcaagtt
     3301 tagcattaat tgcatccctg attccccgtg cctgctcccc ccattcatcc tgatcatccc
     3361 aacgtcctga actgcaacta cccagggctg tagttttatg gcaatttgaa ggatgtaatg
     3421 gcgcacatcc cgccacaaag ctgactaaaa tagccagcat aacgattttc tttaccccca
     3481 tatattgctc ctcaatccat atagatctca gtaccatagc tctgtcagag caaagaaaat
     3541 cttaactaat acaagttaag atttttaata tccactcctc atccctacat tccaggtgat
     3601 ggtggcgtcc actcattatc ctgaactgga gcataagatg cgccatgccc cccggatatg
     3661 cttttctgtt gcctgatata ttcgtggtag tactgttcac tacgctgacg atgtaaatcc
     3721 acatccgggc ggctgattac aaagtgtcca tttccggtta ttccagctgc attatgccag
     3781 gccaccattt ccgcaaatac atcattaaac tgttgatatg agccttccgg ttcaaaaccc
     3841 attcctggct cacgatcaaa caatacagga gcaatcagat tcagcttatc caccagttca
     3901 tttctgtcct gatgatggta gaacaccaca cggtccccat aatccacaca gactaactcg
     3961 ccattcctcc gatcacgaaa acgcacactg ccatctttct gcagacgggc ttcgagaacg
     4021 ccagtgtctt cataaagtgg agttcctccc ggttcacaga gaatcacgca gcggtcggca
     4081 ttcgttgttc gtcgcttgtc tttgcgacga tcacgataat cccagccacg taactgtgat
     4141 aatgcagctc tgtctccctg taccgcctgt tgctccaccc actgccggta ggacaaagga
     4201 taccacttac cctccgcaat cagtgacaac cgctcttcct tcacactctc cttcagcctg
     4261 atcagcgcct gcatacgctg cacttccgca atatggtaat gcagcttacg caattgtgga
     4321 tcccggaact gcacacggat atacgccttg cgccgacggc aggcagcatg aatttcccgt
     4381 aaccgttctc cgtaacgtaa gtcgggttta cgccagtgct ccttccatgc aagataacgc
     4441 gcgcgcaaat cttcccgcgc agctgcacgg gcttcacggc gttcacgacg cagtacagga
     4501 tcgcgccgga agccgggttc cacttcatcg ctggcagcaa gttccggatt gtagcgacat
     4561 tcaggcttca cccgttcaaa tatatctgca cctgcaatct caaatggccc tgcctgcagc
     4621 tcagccctgg ataacgtcaa atccgggtgg atactgctgg ccttcactgg tgtgagctca
     4681 tgattaaacg catccacaat cacaagacca tgatgctgtt tctgcaacat aagcccttta
     4741 cgcgcaaaca tctcatggca cgcctgccag ctgaaattcc cgcccgcgat catatcttca
     4801 atcaacgact cacactgaac agtcgtgtac ccccacagac tttccttatc agaaaacagt
     4861 tcgcgcttta ttgctccctg tggacctgtc cccatccgca tacgatacca ttcgtaatca
     4921 ctgacagcct gagtaccaag attacgtccc cggaatcgtt ctccgggtat aacctgagta
     4981 aaaatatcct tcggtacagg ctgccatcca ccatccagat cctgcatata atccagcgtc
     5041 attaacggcc acacactgtc tgcacgtact ggtgtctgtt gtttatcaaa tccatcatgc
     5101 agtaccagat gcccgtgctg gatccccatc cataatccag cctttgccag agttctgtgc
     5161 acatccaggc aactttccag ctcaccattt ctcgccattt ccggtaagcg ttcggcaaaa
     5221 taacggcagg cgtactgttt cagactctcc gtttctgcca ctttttccgg ccggatatca
     5281 acgtttatgg cttccggatc ataacgaccg ggtgtcccca cctgactgaa aatatctgtc
     5341 ggtacaggct gatattcccc cagtttccgc cccagttttt cagctgtcca tgacggtccg
     5401 aacgaactga gtgcaacacc ttcccgcgct ctgtcccagc catccttcac caccagttcc
     5461 ccttcctgca tcgtgatata cagaccatca ctggccagcc gtttatgcaa agacaaccag
     5521 tcttgtgcgg gctcttcacg cagcccggca atcgacatct gcgcaatata ttccctgaac
     5581 gtctgctttt tcccgcgtcg ccaggcatta cggcgctcac gtaccagcgc tgttttgcgc
     5641 acaatacggt tgccgggtgc atgcacaaag cagccattat caggcgcgaa tccgtgctta
     5701 agttcaagtt cacgacatgc acggctgagt ttttcctggc tccaggaaag ccagttaata
     5761 taaccagttt caggatgtac acgattcacg gcaacatgca catgcagatt atccgtgtcc
     5821 gtatgaacgg cagccacgta ctgatgtttt gagagttcaa gagatttcag tgtgtgacgc
     5881 acacagtcaa aaagctgctc aggacgcggg ctttcatgcg caggccagga aagaatgtag
     5941 tggaaaacgg ggtctgtatc attacgggaa aaaacagctt tgtccgcctt gtactgcatc
     6001 tcttcagcag ctgtttccag tgaattgcag ttatggaaac aggttacgcc atagaaattt
     6061 acccactgac tgccgtcttt catcacatca atcagggaga caaatttttc gttacgcagg
     6121 cgtgttgcat aatcaacaag ccgtctgaaa cggttgcggt gagggacatc actcttgatt
     6181 tcgctgtctt ctgtgagctc attttcctgc acatcatccc gcacagacgt gtacgcaatc
     6241 aggtcttcaa aggaagacct gccgtcacgt cttttgtgcg gaataatggc attcatcagc
     6301 agttatctca gcgtcccatt aagtctcttc gcagagcatt gacatattca gtaatggcaa
     6361 caagaacctc tgcatactcc ttatcaccag tgcgttttcc ctccacaaac agatgcttct
     6421 gaaggcggcc aagacgcata atctcatcaa tcatacggtc gtcagtacgg cagttaacca
     6481 ccttatccag tgaagagctt cgtataaatg ttgaaaccgt ctgtccactt gactccgctt
     6541 tttctcttac gatttcagcc tcatcctccg tgaatctcgc cgtgaatttt acaattctct
     6601 tcctgttctc actgcccgtt cgggttttct tctcactcat ggtctgctcc ttaccggggt
     6661 gtcggggcaa agccctgacc aggtggggaa tgtctgagtg cgcgtgcgcg gtccgacatt
     6721 cccacatcct gtcccgtttt tcaggccatt atagcctgtt atttttgtgc atcatagtct
     6781 tatgcagacg gcagaggcag ggcgcagccc
//
LOCUS       DAZFRE010000443         5961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.186, whole genome shotgun sequence.
ACCESSION   DAZFRE010000443 DAZFRE010000000
VERSION     DAZFRE010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.186"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..135
                     /locus_tag="ACHKUI_005482"
     CDS             <1..135
                     /locus_tag="ACHKUI_005482"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6601439.1"
                     /translation="LTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH
                     "
     gene            325..1464
                     /locus_tag="ACHKUI_005483"
     CDS             325..1464
                     /locus_tag="ACHKUI_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6601440.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIEQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1461..2075
                     /gene="prfH"
                     /locus_tag="ACHKUI_005484"
     CDS             1461..2075
                     /gene="prfH"
                     /locus_tag="ACHKUI_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6601441.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLSSGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2132..3589)
                     /gene="pepD"
                     /locus_tag="ACHKUI_005485"
     CDS             complement(2132..3589)
                     /gene="pepD"
                     /locus_tag="ACHKUI_005485"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6601442.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3850..4308
                     /gene="gpt"
                     /locus_tag="ACHKUI_005486"
     CDS             3850..4308
                     /gene="gpt"
                     /locus_tag="ACHKUI_005486"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6601443.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            4400..5644
                     /gene="frsA"
                     /locus_tag="ACHKUI_005487"
     CDS             4400..5644
                     /gene="frsA"
                     /locus_tag="ACHKUI_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6601444.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5702..>5961
                     /gene="crl"
                     /locus_tag="ACHKUI_005488"
     CDS             5702..>5961
                     /gene="crl"
                     /locus_tag="ACHKUI_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020236109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6601445.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQ"
BASE COUNT         1435 a         1466 c         1575 g         1485 t
ORIGIN      
        1 cttacggtgg acgccagcat aaccgcaaaa cccttttttg aacgttatgg ttttcagaca
       61 gttaagcagc agcgcgttga atgccgggga acgtggttta ctaattttta tatgcgatat
      121 aaaccgcaac attaaatcca gcttgcaatg aaaataacgc ccgtctagta tgtgcgcgtt
      181 ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca atgactcttt
      241 gcagaccttt ccaggattaa ttcttttttc ttgccctgga ttcgtctgcc atttcctgat
      301 ttttattcat aaatggaatg gtacatgggc aaatatattc gtcccttatc cgatgcggta
      361 tttaccatcg catctgatga cctgtggatc gagagtttag cgatccaaca attacacacc
      421 acggcaaatt tacccaacat gcagcgcgtt gtggggatgc cagatttaca tcccggacgc
      481 ggctacccga ttggcgcagc gttcttctct gttggtcgtt tttacccggc actggtcggc
      541 aatgatatcg gctgcggcat ggcgctatgg caaacagata ttctcgctcg caaatacaac
      601 gccgataagt ttgaaaagcg attatcagat ctggatgacg ttgctgaaga aagctggctg
      661 gaggaaaacc tgccgtcagc atttgcacag catccgtggc gcaactcgct tggctccatc
      721 ggtggcggta accacttcgc agaactgcaa cagattgagc aaattatcga cgcggaactg
      781 tttgcactgg ctggtctgga tgcgcagcat ctgcaactgc tggttcatag cggctcgagg
      841 ggattggggc agtctatttt acagcggcat attgcctcgt tttcgcatca tggtttgcct
      901 gaaggcagtg acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg
      961 cgtattaatc gccagctgat cgctttgcgc ataatgcaac aagttaaggc caccggaagt
     1021 ccggttctgg atgtggcgca taactttgtt agcgcgtgcc aaatcggcga tcaacagggc
     1081 tggttgcatc gtaaaggcgc cacgccggat gacaacggtc tggtgattat tcccggttca
     1141 cgcggcgatt actcctggct ggttaaacct gttgcgaacg aaaaaacgct ccactcgctg
     1201 gcgcatgggg cggggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa
     1261 tacacggcga cgcaactctc gcggactgaa cttggcagtc gggtaatttg tcgcgataaa
     1321 caactcatct ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt
     1381 ctggtgctgg ctgggttaat tattcctgtc gcgcgactgc gcccggtgct gacgctcaaa
     1441 aacagtggag ggaaaaaagg atgatcttgc tacaactctc ctctgctcag gggccggaag
     1501 aatgttgtct cgcagtgaaa aaagcactgg acaggctgat taaagaagct acccgacagg
     1561 acgtcgcggt aacggtgctg gaaacagaaa cgggtcgcta ctctgacaca ctgcgttcgg
     1621 cgctgatttc tctggatggc gataacgcat gggctttaag cgaaagctgg tgcggcacta
     1681 ttcagtggat ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg
     1741 gcattgggcg ttttaccgct gatgagcagg aacaatcgga tgcaatccgt tatgagacgc
     1801 tgcgttcgtc agggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     1861 cgcatctgtc atccggtatt agcgtgaagg ttcagtcaga gcgcagtcag catgctaaca
     1921 aacggctggc gcgattactg attgcctgga agctggaaca acagcaacag gaaaatagcg
     1981 cggcgctgaa atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     2041 gaacgttcac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     2101 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     2161 cagtcagcag tgtccagtaa tggccaacgc tttcgatatg aacttgctca tccggagagt
     2221 gtggaccggt gatggttggc ccgatagaca ccatgtccat ttccggatac ggttttttga
     2281 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgttttg ttgaacagac
     2341 gctgataggt ttcacgtacc agatgcatta ccggagagtt agcgtccggc tgccagccag
     2401 gatatgcgcc ttttgcttct gttttcgcac ctgccagttt acccagtgaa tccagcatgc
     2461 tcactacgta gtctttaccg ctgtcgatca gagaacggat caggcagtga atttctacgt
     2521 tattgtcggt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     2581 cgtcggagtt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     2641 gagatttcgc aatcagggca gctttatcgt tcgcaacaga gtccagcagc aatgccagat
     2701 ttttctcttt ctctgccagt tcgtttttca ggatctcctg ataggtatcc accagagatt
     2761 tcagggcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     2821 cgttacgcag tgtgccgccg ttgaaatcaa taagacgcag gtccagttct tctgcatgac
     2881 ccgccaggaa gcgcaccaac agtttgttgg cattacccag gccaacgtgg atttcaccgc
     2941 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     3001 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat acctcccgca caacccatgt
     3061 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     3121 tctgtaagcc aaaagcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     3181 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     3241 cgttatccgc acccagcgtg gtgccgcgtg ctttaaccca ttcgccatca atataaggct
     3301 ggataggatc tttagtgaag tcatgcacgg tgtcgttgtt tttttgcggc accatatcga
     3361 ggtgggcctg caagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     3421 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     3481 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     3541 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     3601 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     3661 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     3721 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     3781 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3841 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3901 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3961 ggcggtctgg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     4021 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     4081 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tagataccgg tggtactgcg
     4141 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggcc
     4201 ggtcgtccgc tggttgatga ttatgttgtt gatatcccgc aaaatacctg gatcgaacag
     4261 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4321 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4381 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4441 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt agtcacggcg cacaaccgcc
     4501 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4561 gatgtggatc tggcgcggca ttgacccccg tgaaatcctc gatgtccagg cgcgtattgt
     4621 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgcgg
     4681 cggcaactgg atttatgagt gggctaccca ggcgatggtg tggcaacaaa aagcctgtgc
     4741 ggaagaagat ccgcaactca gcggtcgaca ctggctgcat gcggctacgt tgtacaacat
     4801 tgctgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttgtcaaa
     4861 ccgcgcctat gaagaggccg ctcagcgtct gccgggcacg atgcgacaga tggagtttac
     4921 cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt
     4981 cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta
     5041 tgaacgttat tttgcgccgc gcggcattgc tatgctgact attgatatgc cgtcggtggg
     5101 cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgttttaaa
     5161 ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt
     5221 cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga aagtggttgc
     5281 ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc
     5341 ggaaatgtat cttgacgttc tggccagtcg tttagggatg catgatgctt ccgatgaagc
     5401 gttgcgagtg gagttgaatc gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg
     5461 cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc
     5521 acgcttaatc acgtcgtcat ctgctgacgg taaattatta gagatcccat ttaacccggt
     5581 gtatcggaat tttgacaaag gccttcagga aatcacagac tggatcgaaa aacgtttgtg
     5641 ttaaaaattt gctaaatttt accaatttgg taaaacagtt gcatcacaac aggagattgc
     5701 aatgacgtta ccgagtggac acccgaagag cagattagtc aaaaaattta ccgcactagg
     5761 cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt
     5821 atgcgtcaac gtgaaaccgg caccggaagt gcgtgagttc tggggctggt ggatggaact
     5881 ggaagcgcag gaatcccgtt ttacctacag ttaccagttt ggtctgttcg ataaagcagg
     5941 cgactggacg agtgttcaga t
//
LOCUS       DAZFRE010000444         5242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.195, whole genome shotgun sequence.
ACCESSION   DAZFRE010000444 DAZFRE010000000
VERSION     DAZFRE010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.195"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            72..1196
                     /locus_tag="ACHKUI_005489"
     CDS             72..1196
                     /locus_tag="ACHKUI_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO6601446.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1177..3480
                     /gene="trbC"
                     /locus_tag="ACHKUI_005490"
     CDS             1177..3480
                     /gene="trbC"
                     /locus_tag="ACHKUI_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO6601447.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            4344..5159
                     /gene="sul2"
                     /locus_tag="ACHKUI_005491"
     CDS             4344..5159
                     /gene="sul2"
                     /locus_tag="ACHKUI_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO6601448.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         1310 a         1478 c         1306 g         1148 t
ORIGIN      
        1 gaaaccaccg aaaccaccga aaccaccgaa accaccgaaa aacagcatcc cgccgacaag
       61 gaaaaagaac aatgaccatc cactacactg caacactgaa taataaacac ctgtccctga
      121 tcgccctgta ccctgacggt aatcgggagg tgattgccat cctgccagtc acagaactgg
      181 gcgcactgat taaccgtaat aacaggctga tgcacctgtc cttcagttcc ccttttcgcg
      241 aaataaccga aacggtgctt gttgataccg ttgatgcaga acgtcccgga catcacatcg
      301 gcagaatgct ctcagcgatt gatgcctgcc tggatgccga atatgccgga aaaggaaaac
      361 atccctcccg gaaaaatggc ttctggcgga cactgatgtg tggctttatc gccggaattg
      421 ctctgccggc gacagttgca ggttaccact ggatgaaggc agagccccct tccacgctgg
      481 cttaccctac atctcaggaa tggagagctg aaccagtaac cgacacagag acaccacgcc
      541 cacatacgga acatcatgtt aacagcatcc cggcccgccc ggctcccaca cagcaggaac
      601 cttcttctgc ccccgccccg acggcagtga cggaagaaca acacgttcag gccgctgagg
      661 cccgccgtaa tctggccaca gtacttaaac gcaacgcaga ccgggggatg ttcaccatta
      721 acctgtccac tggtcatgaa cgcacgctgt acgccttcct tgaccccgca tgcatcaact
      781 gccgtctgct ggaacccgcg cttaagcaac tggcccggga attcagcgtg gtgatttatc
      841 cggtctccgt gattggcggg gagtccagta caaagcggac tgctccgctg ctgtgtgaac
      901 cggactcaca aaaacgggcg accggatggc atcagctgta ttccgctgac agtggtatgg
      961 cccccaccgg cgatgcaccg gccgcacagg atggacagtg tctgaccgcc gcacgtgctg
     1021 ccatcgatgt caataaccag gctttccgcc agttcggttt tggcggaacc ccctgggtgt
     1081 tatcagatac cggctggcac ctgccgtccg gcatcctgaa agacgccact tccgttcgcc
     1141 tcttcctgaa agcaacagat agcgaggagt atcacaatga gccagcacca cgttaatcct
     1201 gacctcatac accgcacggc ctggggaaac ccgttgtgga atgcactgca taacctgaac
     1261 atcactggtc tgtgtctggc cggcagtatc attacggcgc tgatatggcc actggccctt
     1321 cctgtctgcc tgctgttcac cctggtcacc tccgtgatat tcatgctgca acgctggcga
     1381 tgccccctgc gtatgcccat gacactcagc cttgatgacc cctcccagga cagaaaggtc
     1441 aggagaagcc tcttcagctt ctggcccacc ctgtttcagt atgaagcgga tgaaacattc
     1501 cccgcccggg ggatttttta tgtcggttac cgccgtatca acgatatcgg tcgtgagctc
     1561 tggctgagca tggatgacct cacccggcat gtcatgtttt ttgccaccac cggtggcggt
     1621 aaaacagaaa ccacctttgc ctggctgctc aacccgctct gctggggaag aggctttacc
     1681 tttgttgacg gaaaggcaca gaacgatacc acgaggacaa tctggtatct ctcccgacgt
     1741 tttggcaggg aggatgatat cgaagtcatc aacttcatga acggcggaaa atcccgcagt
     1801 gaaatcattc agagcggcga aaaatcccgc ccacagtcaa acacctggaa cccgttcgca
     1861 ttcagcaccg aagccttcac cgccgaaacc atgcagtcca tgctgcccca gaacgttcag
     1921 ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg ccctggtctt tggcacaaaa
     1981 ttctggtgcg tccgggaagg taaaaccatg tccctgcaga tgttacggga acatatgacg
     2041 ctggaaggga tggcaaaact ctattgccgt ggccttgatg accagtggcc ggaagaagct
     2101 atcgcaccac tgcgaaacta cctgcaggat gtcccgggat tcgatatgtc actggtgaga
     2161 acgccgtcag cctggactga ggagccccgt aaacagcatg cttatctctc cggtcagttc
     2221 tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg tctttgcggc agatgccggc
     2281 gacatcgata ttcgcgacag tattcacagc gaccgcattc tgattgtgat gatccctgca
     2341 ctggacacat cggctcatac cacgtcggca ctcggtcgta tgttcgtcac ccagcagagc
     2401 atgctcctcg cccgtgacct gggctaccgg ctggaaggca cagatgcaca gaccctggaa
     2461 gtcaaaaaat acaaaggcag cttcccctat atctgtatcc ttgacgaagt cggtgcctac
     2521 tacacagacc gcattgctgt ggaggccacc caggtacgtt cactggagtt ttccctgata
     2581 atgacggcgc aggatcagga gcgaattgaa gggcagacct ccgccaccaa caccgccaca
     2641 ctgatgcaga acgccggcac caaattcgcc ggacgcattg tcagtgacga caaaacggca
     2701 agaacggtga aaaatgccgc cggtgaagag gcacgggcca gaatgggcag cctgcagcgc
     2761 cacgatggtg tgatgggtga gtcctgggtg gacggtaacc agatcaccat ccagatggaa
     2821 agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg gtgagttctt taccgttttc
     2881 cagggggatg tggtgccgtc agcctctgtg tatattccgg acagcgagaa aagttgcgac
     2941 agtgatccgg tggtcatcaa ccgctatatt tccgttgaag ccccccggct ggagcgtctc
     3001 cgccgtctcg tccccaggac agtgcagcgc aggctcccca caccggagca tgtcagcagc
     3061 atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc gtaaaaaccg tacagagcct
     3121 taccgtatca ttgatacatt ccagcgccag ttggcaacag cgcagacatc actggatctg
     3181 cttccgcaat atgatacaga tatcgaatcc cgggcaaacg aactgtggaa aaaggctgtg
     3241 cacactatca ataacacaac cagggaagaa cgccgggtat gttatatcac actgaatcgt
     3301 ccggaagagg aacactccgg tcctgaagat ataccttcag tgaaggcgat tctgaatact
     3361 ctgctccccc tggagatgct gttacctgta ccggatctaa ctgcatcccc gccccataaa
     3421 aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga gtcgtaaaaa aagattctga
     3481 taacacctgt atggctgatt ttaataacaa cctttacgcg attattaatc tcctccgctc
     3541 accatagtaa gcggaggatt caggatttca atccatcacc agaggtacaa taaatgtgta
     3601 ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc ggagaagata
     3661 tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggaca ctgatggtgc
     3721 ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga tgggacagag
     3781 aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc gaaatactgg
     3841 gtaagctccc cataataaat aaagtcacaa aactgataaa atcataatct acggatttta
     3901 atcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga aaacaaatat
     3961 gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt aatagattca cttctatgga
     4021 tattggtatg aattcccaga tcccactcta ttgtaaacaa gacattttta tcttttatat
     4081 tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg
     4141 cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt
     4201 tcctggcttt gcttccagat gtatgctatt ctgctcctgc agctaatgga tcaccgcaaa
     4261 caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac tcatgctcga
     4321 ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     4381 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     4441 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     4501 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     4561 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     4621 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     4681 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     4741 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     4801 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     4861 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     4921 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     4981 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     5041 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     5101 ccttgcgcga cgggctggcg gtattggcgg cgctaaaaga aaccgcaaga attcgttaac
     5161 tgcacattcg ggatatttct ctatattcgc gcttcatcag aaaactgaag gaacctccat
     5221 tgaatcgaac taatattttt tt
//
LOCUS       DAZFRE010000445         4615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.201, whole genome shotgun sequence.
ACCESSION   DAZFRE010000445 DAZFRE010000000
VERSION     DAZFRE010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.201"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            <1..1213
                     /locus_tag="ACHKUI_005492"
     CDS             <1..1213
                     /locus_tag="ACHKUI_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO6601449.1"
                     /translation="YRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGIL
                     MTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRK
                     GNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTM
                     GGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAAT
                     AGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGMAMEEGCGIYRTPELMQK
                     TIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAH
                     QRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKA
                     EAANKKEKANG"
     gene            1206..1940
                     /gene="frdB"
                     /locus_tag="ACHKUI_005493"
     CDS             1206..1940
                     /gene="frdB"
                     /locus_tag="ACHKUI_005493"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6601450.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1951..2346
                     /gene="frdC"
                     /locus_tag="ACHKUI_005494"
     CDS             1951..2346
                     /gene="frdC"
                     /locus_tag="ACHKUI_005494"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6601451.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2357..2716
                     /gene="frdD"
                     /locus_tag="ACHKUI_005495"
     CDS             2357..2716
                     /gene="frdD"
                     /locus_tag="ACHKUI_005495"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6601452.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2779..3912
                     /locus_tag="ACHKUI_005496"
     CDS             2779..3912
                     /locus_tag="ACHKUI_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HFO6601453.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4001..4534
                     /gene="blc"
                     /locus_tag="ACHKUI_005497"
     CDS             4001..4534
                     /gene="blc"
                     /locus_tag="ACHKUI_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6601454.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4531..>4615)
                     /locus_tag="ACHKUI_005498"
     CDS             complement(4531..>4615)
                     /locus_tag="ACHKUI_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HFO6601455.1"
                     /translation="GESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT         1132 a         1213 c         1232 g         1038 t
ORIGIN      
        1 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
       61 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctaccagg
      121 ttccggtatc ctgatgaccg aaggctgccg cggtgaaggt ggtattctgg tcaacaaaaa
      181 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
      241 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
      301 gcgtaaaggc aacaccatct ccacgccacg tggcgatgtg gtttacctcg acctgcgtca
      361 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
      421 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
      481 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
      541 tgaatgttcc tctgttggtc tgcacggtgc gaaccgtctg ggctccaact cgctggcgga
      601 actggtggtc ttcggtcgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
      661 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
      721 tctggttaac caggatggcg gcgaaaactg ggctaagatc cgcgacgaaa tgggcatggc
      781 catggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
      841 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatt accgacactt ccagcgtgtt
      901 caacaccgac ctgctctaca ccattgagct gggccacggt ctgaacgttg ctgaatgtat
      961 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgcg caccagcgtc tggacgaagg
     1021 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
     1081 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     1141 tgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     1201 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     1261 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     1321 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc tggtcctgcc
     1381 gtatggcgat ttgtggctcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     1441 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     1501 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     1561 cgtacatcat cggcaacgcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     1621 agatggcgaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     1681 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     1741 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     1801 agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc ccgaaacacg
     1861 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     1921 cgactctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     1981 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     2041 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcggcct gtttgccctg
     2101 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     2161 atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg gtttgagctg
     2221 gcaccaaaag cagccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     2281 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     2341 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     2401 gggcctcttc ggggccggtg gtatgtggag cgccatcatt gcgccggtga tgatcctgct
     2461 ggttggtatt ctgctgccgc tagggctgtt tccgggtgat gcgctgagct acgagcgcgt
     2521 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     2581 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtacc
     2641 tgcgggtaaa tgggttttct acggtctggc tgctatcctg acagttgtca cactgattgg
     2701 tgtcgttaca atctaacgca tcgccaatgt aaatctggcc cgcctatggc gggccgtttt
     2761 gtatggaaac cagaccctat gttcaaaacg acgctctgca ccttattaat taccgcctct
     2821 tgctccacat ttgccgcacc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     2881 cttatagagc aacaaaagat ccccggtatg gcggtagcgg taatttatca gggtaaacct
     2941 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     3001 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     3061 gctcgagggg aaatcaagtt aagcgatccc acaacaaaat actggcctga acttaccgct
     3121 aaacagtgga atgggatcac actattacat ctcgcaacct acaccgctgg cggcctgcca
     3181 ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     3241 cagcctgcat gggctcctgg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     3301 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa aactcgtgtc
     3361 ttccagccac tcaaactcaa tcatacgtgg attaatgtac cgtccgcaga agaaaagaac
     3421 tacgcctggg gatatcgcga aggtaaggca gtacatgttt cgccaggggc gttagatgct
     3481 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     3541 aaaccccttg atatcaatga gaaaacactt caacaaggga tacaactggc acaatctcgc
     3601 tactggcaaa ccggcgatat gtatcagggt ctgggctggg aaatgctgga ctggccggta
     3661 aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc acgccccgta
     3721 aaaccgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     3781 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     3841 ctggctaaca aaaactaccc caatccagcg agagtcgccg ccgcctggca gattctcaac
     3901 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctccgc ttttccttgc
     3961 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     4021 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tcccctacgc cgccgcgtgg
     4081 cgtgaccgta gtgaataatt tcgacgctaa acgttatctc ggtacctggt atgagattgc
     4141 ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
     4201 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag aaatgtggca
     4261 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
     4321 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
     4381 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     4441 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     4501 taagtttatt tgggtacagc agcccggtag ttaatgagtg ctgagtttca gaccaataat
     4561 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccg
//
LOCUS       DAZFRE010000446         2936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303T44177
            SAMN43283683-rid23073753.guided.232, whole genome shotgun sequence.
ACCESSION   DAZFRE010000446 DAZFRE010000000
VERSION     DAZFRE010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283683
            Sequence Read Archive: SRR30325481
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351743.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351743.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:57:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,166
            CDSs (with protein)               :: 5,166
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 101 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 47 of 250
            Pseudo Genes (multiple problems)  :: 57 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283683-rid23073753.guided.232"
                     /serotype="O 75:H 5"
                     /isolate="2303T44177"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2k"
     gene            132..1091
                     /locus_tag="ACHKUI_005499"
     CDS             132..1091
                     /locus_tag="ACHKUI_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2k subunit A"
                     /protein_id="HFO6601456.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSQEFTIDFSTQQSYVSSLNSIRT
                     AISTPLEHISQGATSVSVINHTPPGSYISVGIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISLPGVTTISMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTREASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPEDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE"
     gene            1103..1372
                     /gene="stxB2"
                     /locus_tag="ACHKUI_005500"
     CDS             1103..1372
                     /gene="stxB2"
                     /locus_tag="ACHKUI_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit B"
                     /protein_id="HFO6601457.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCASGSGFAEVQFNND"
     gene            complement(1475..1606)
                     /locus_tag="ACHKUI_005501"
                     /pseudo
     CDS             complement(1475..1606)
                     /locus_tag="ACHKUI_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1937..2016
                     /locus_tag="ACHKUI_005502"
                     /pseudo
     CDS             1937..2016
                     /locus_tag="ACHKUI_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067459.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            2002..2878
                     /locus_tag="ACHKUI_005503"
                     /pseudo
     CDS             2002..2878
                     /locus_tag="ACHKUI_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799662.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          813 a          593 c          737 g          793 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca cacttatttt ctcgggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aacacctgta tatgaagtgt atattattta aatgggtact gtgcctgtta ctgggttttt
      181 cttcggtatc ctattcccag gagtttacga tagacttttc gactcaacaa agttatgtat
      241 cttcgttaaa tagtatacgg acagcgatat cgacccctct tgaacatata tctcagggag
      301 ctacatcggt atccgttatt aatcatacac caccaggaag ttatatttcc gtaggtatac
      361 gagggcttga tgtttatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccgggttc gttaatacgg caacaaatac tttctacaga ttttcagatt
      481 ttgcacatat atcattgccc ggtgtgacaa ctatttccat gacaacggac agcagttata
      541 ccactctgca acgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt tcagtggtaa tacaatgacc agagaggctt
      661 ctagggctgt cctgcgtttt gtaaccgtca cagccgaagc cttacggttc aggcagatac
      721 agagagaatt tcgtcaggca ctgtctgaaa cagctcctgt ttatacgatg acgccggaag
      781 acgtggacct cactctgaac tgggggcgaa tcagcaatgt gcttccggag tatcggggag
      841 aggatggtgt cagagtgggg agaatatcct ttaataacat atcagcgata cttggtactg
      901 tggccgttat actgaattgc catcatcagg gcgcgcgttc tgttcgcgct gtgaatgaag
      961 agagtcaacc agaatgtcag ataactggcg acaggcccgt tataaaaata aacaatacat
     1021 tatgggaaag taatacagca gcagcgtttc tgaacagaaa gtcacagtct ttatatacaa
     1081 cgggtgaata aaggagttaa gtatgaagaa gatgtttatg gcggttttat ttgcattggt
     1141 ttctgttaat gcaatggcgg cggattgtgc taaaggtaaa attgagtttt ccaagtataa
     1201 tgaggatgac acatttacag tgaaggttga cgggaaagaa tactggacca gtcgctggaa
     1261 tctgcaaccg ttactgcaaa gtgctcagtt gactggaatg actgtcacaa tcaaatccag
     1321 tacctgtgca tcaggctccg gatttgctga agtgcagttt aataatgact gaggtataac
     1381 ctgattcgtg gtatgtgggt aacaagtgta atctgtatca caattcagtc agttgactgt
     1441 tgcctgacag gctgagcgtt tgttaaaaaa tttacgtatg gtgaatcccc ctgtgcggag
     1501 gggcaatcag caagtagata tatgggataa ttgcggattc aggtgctgat actgaattca
     1561 ccgggaggca cccggaacca tgcaggtaag cagtgtaaat gttcacataa tcaaatcccc
     1621 tcaccggagg ggatttttac gccaaaaaat gccgcgtatg gcgttgagat tgacagcctg
     1681 gtgctggaga caaatgtacc gacatcgtaa taaaaaaaag ccagcataga agagaacggg
     1741 aagactagtc tgacgctggc gtggggatat tccccgtgga taaatgatat gttacacata
     1801 tcgggagtct ttttatataa aagtgataac cattgtcaat cataacggtc aggaatgatg
     1861 acgtttatgc atcagggcca tcagtaatta acgggtggct tttttttgtt gctgtcagct
     1921 tccggagttc ggagatatgt acctgatgga aaaaatcaca actgacgttg cttacggcac
     1981 atcagcaggt agtgcaggtt tctgcagtgg ctggaccact gtctggcccg gttatggcgg
     2041 gtgctgaaac ctgccggaag cttttacctg ttctgtggag atcgctaggc atctgatatc
     2101 gagataataa tgcgtgagcg tttcaacgtg cttaaccata tcatctgggc gaaaccgtcc
     2161 gggcgctgga acgggccagc aaggagagtc tacggtgtat ttcccggcta cagcgttcat
     2221 tctgcttgtt gaacattatc agggaccata ttggccctaa aatgatggct atgcggcaaa
     2281 ggggcgcgag cttaaacagc acgtcatggc cccgctgatt tcttactttc gtgatgcgcg
     2341 tgattcactg ggggtaacat cgaaacagat agcggaagcc accggaaaga aaaacatggc
     2401 gtcacactgg tttggtacca gtcagtggca gttgccgaac gagggtgatt acagaaaact
     2461 gcagttgtta tttgcacgtg tagcagcaga aaagcatcag cgcggagaac tggaaaaacc
     2521 acatcaccag ctggtcagcg catacagtga actgaaccgg caatatgcca gcctgctggc
     2581 agagtacaaa tctttacggc gttatttttc cgtatcggct gctgttcctt atacggatgt
     2641 ctggacgtac aagccagtgc agtattatcc gggcaaacat ccctgtgaaa aaccagcgga
     2701 tatgttacgt cagataatta ccgccagcag tcgtccggga gacttggttg tcgacttttt
     2761 tatggggtca ggctccacaa ttaaagcagc aatgacgctg gggcgtcggg caattggtgt
     2821 ggaactggaa aaagagagat ttaatcaaac tgtaactgta atagaaaata atctttaaac
     2881 acgctttctg ttatttatta attattgaaa taataaaaac atataagtat atttac
//
